Syngenta Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, SK10 4TJ, U.K.
Received July 28, 2004; accepted September 20, 2004
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ABSTRACT |
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Key Words: bisphenol A; uterotrophic; gene expression; progesterone receptor.
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INTRODUCTION |
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MATERIALS AND METHODS |
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Animals. Immature Alpk:APfSD (Wistar derived) rats were obtained from the AstraZeneca breeding unit (Alderley Park). Rats were 1819 days old (body weights 3745 g), on arrival and were acclimatized for one day before dosing. Animal studies were performed in accordance with the U.K. "Animals (Scientific Procedures) Act." Rats were housed (up to five per cage) in polypropylene cages. Rat and Mouse No. 1 diet (Special Diet Services Ltd., Witham, Essex, U.K.) and water were available ad libitum. The phytoestrogen levels in the diet have been described earlier (Odum et al., 2001). Animal care and procedures were conducted according to in-house standards as described previously (Odum et al., 2001
).
Uterotrophic assays. Uterine growth and gene expression following BPA treatment was investigated in three immature rat uterotrophic assays, conducted according to current OECD protocols (Kanno et al., 2003a,b
). E2 was used as a reference estrogen in all studies. All compounds were given daily by gavage in AO (5 ml/kg) beginning on PND 1920. First, a time course was conducted where BPA (800 mg/kg/day) and E2 (0.4 mg/kg/day) were administered at doses previously shown to give a uterotrophic response after three days (Ashby and Tinwell, 1998
; Odum et al., 1997
). Controls received AO only. Rats (five per group) were killed 4, 8, and 24 h after a single dose of compound and 24 h after the last of three daily doses (72 h after the first dose). Second, two dose-responses to BPA (0.002800 mg/kg/day) were conducted, E2 (0.4 mg/kg/day) was used at a single dose level, controls received AO. Rats were killed 4 h after a single dose of compound and 24 h after the last of three daily doses (72 h after the first dose) in both experiments. The first experiment contained five rats per group and the second experiment 10 rats per group, but they were otherwise identical. Animals were killed using overexposure to halothane (Concord Pharmaceuticals, Dunmow, Essex, U.K.) followed by cervical dislocation. Uteri were removed, blotted and weighed individually, frozen in liquid N2 and stored at 70°C.
Real-time RT-PCR. The frozen uteri were pooled according to group and ground to a powder in liquid N2 using a dismembrator (Brownes, Reading, U.K.). Gene expression was determined by the quantitative real-time reverse transcriptase polymerase chain reaction assay (RT-PCR) on uterine RNA. Three genes regulated by estrogen were measured: PR, complement component 3 (C3) and lipocalin 2 (lipocalin). The first two contain estrogen response elements (Klinge, 2001) and are known to be estrogen-responsive in the rat uterus (Kraus and Katzenellenbogen, 1993
; Sundstrom et al., 1989
). The third gene, lipocalin, is a secreted iron trafficking protein that mediates iron delivery to differentiating epithelial cells (reviewed in Kaplan, 2002
). It was identified as being an estrogen-responsive gene by microarray analysis of E2-induced uterine growth in the immature mouse uterus (Moggs et al., 2004
), and was upregulated at similar times to PR. Two genes were used for confirmation that RNA concentrations were equal in control and test samples: 18S rRNA (using commercial primers and probes supplied by Applied Biosystems, Foster City, CA) and RNA polymerase II large subunit (RPB1), which was also identified by the same microarray analysis (Moggs et al., 2004
) as a gene which was unchanged after estrogen treatment. The nucleotide sequences for the primers and probes (supplied by MWG Biotech, Milton Keynes, U.K.) used in the real-time RT-PCR assays were generated by "Primer Express" software (Applied Biosystems) and are shown in Table 2.
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Reactions were carried out in triplicate in each run, "no-template" controls were used to check for contamination. Each run contained a standard curve consisting of six points prepared from dilutions of a positive control sample for which all of the genes studied were known to be expressed. A standard curve was plotted of Ct (threshold cycle) against log RNA concentration (in arbitrary units). The control and test sample RNA concentrations were then interpolated from their Ct values and relative gene expression calculated (test divided by control). Real-time RT-PCR was carried out on 25 separate occasions for each gene. Gene expression was reproducible within experiments, as illustrated for PR expression (Table 3).
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SDS-PAGE and Western blotting were performed using a "Novex" gel system with 412% tris-glycine gels (supplied by Invitrogen) according to the method described by Williams et al. (2001). A uterine sample prepared from a mature female rat was used as a positive control for PR as it is constitutively expressed in adult females (Cotroneo et al., 2001
). Gels were loaded with 60 µg protein/lane. Loading consistency was checked by staining the gel with a general protein stain ("SimplyBlue" supplied by Invitrogen) after blotting and was acceptable in all cases. A rabbit polyclonal primary antibody to PR (sc-7208) was purchased from Santa Cruz (CA) and used at a 500x dilution. Donkey anti-rabbit IgG-HRP linked secondary antibody was purchased from Amersham Biosciences (Amersham, U.K.) and used at a 2500x dilution. Proteins were detected by chemiluminescence using the "ECL Western Blotting Analysis System" from Amersham Biosciences. PR was quantified by densitometry.
Statistical analysis. In the time course experiment, uterine weights were analyzed by variance and by covariance with the terminal body weights (Shirley, 1996). A representative run was selected for the expression of each gene, and that was then analyzed by variance following logarithmic transformation. Differences from control values were assessed statistically using a two-sided Student's t-test based on the error mean square from the analysis of variance. Statistical significance for these gene expression data should be treated with caution because samples were pooled according to group; consequently, the statistical analyses reflect variability between repeat measurements of a sample, rather than representing interanimal variability.
In the two dose-response experiments, uterine weights from the two experiments were analyzed by variance and by covariance with the terminal body weights (Shirley, 1996). The data from both studies were then combined using a mixed model analysis of variance. Differences from control values were assessed statistically using a two-sided Student's t-test based on the group experiment interaction mean square. Analyses were carried out as described in SAS (1999)
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RESULTS |
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Western analysis of PR protein expression confirmed that the increased transcription of PR RNA was followed by translation (Fig. 2). Although a single gene encodes PR, two isoforms of the receptor (A and B) are produced under the control of distinct promoters (Graham and Clarke, 1997). Both forms of the PR receptor were increased from 4 h onwards (the point at which PR RNA expression was greatest), with maximal protein expression at 72 h (Table 4, Fig. 2). Ilenchuk and Walters (1987)
reported a ratio of A/B in mature female rats of
3. In the present study the ratio in control immature female rats was
56. Cotroneo et al. (2001)
also showed that PR A and B were increased in rat uterus after genistein and estradiol benzoate treatment.
Dose-Response Studies
Administration of BPA over the dose range 0.002800 mg/kg gave an increase in uterine weight 4 h after a single dose of between 200800 mg/kg. Three daily administrations of the same dose-range of BPA gave increased uterine weight over the same dose range, with statistical significance observed for the doses 400800 mg/kg (Table 5, Fig. 3). The lowest positive uterotrophic dose of BPA was 200 mg/kg (Table 5). E2 (0.4 mg/kg) increased uterine weight at the same time points and the magnitude of the uterotrophic response was similar to that in the previous experiment (Tables 4 and 5). There were no effects on body weight after any treatment (Table 5).
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Statistically significant changes in the expression of PR, C3, and lipocalin RNA were generally seen at doses of BPA that were also uterotrophic. PR expression was increased 3-fold 4 h after a single dose of 200800 mg/kg BPA. Lipocalin expression was increased 34-fold 4 h after a single dose of 200800 mg/kg BPA. No statistically significant changes in PR or lipocalin RNA levels were noted at doses below 200 mg/kg BPA at either time point. Changes in C3 expression are particularly interesting. At the 4 h time point an increase of 2.5-fold was seen after a single dose of 20 mg/kg BPA, rising to 6-fold for both the 800 mg/kg dose of BPA and the E2 positive control at 4 h. However, at the 72 h time point, where C3 expression was increased >500-fold by E2, statistically significant increases in C3 expression were only observed for doses of BPA that were also statistically significant in the uterotrophic assay (400 mg/kg and 800 mg/kg BPA; 4-fold and 83-fold increases in C3 expression, respectively; Table 5, Fig. 3).
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DISCUSSION |
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Immediate (4 h) increases in uterine weight are associated with imbibition of fluid. The increases seen for BPA were statistically significant between 200800 mg/kg BPA, with an increase of similar magnitude seen for E2 (Table 5). Expression of lipocalin and PR was increased at the same time as this imbibition (Table 5 and Fig. 3), although a causal relationship is not implied. In contrast, the expression of C3 at 4 h is rather perplexing. The time course for this gene (Fig. 1) indicates major expression between 2472 h, with expression levels at 72 h as high as 1700-fold control levels. Small increases in C3 expression (26 fold) at 4 h were seen for doses of BPA between 20800 mg/kg, and for E2 (Fig. 3). However, the early expression of C3 may not be functionally related to the expression occurring at 72 h. For example, the fold increase at 4 h in C3 expression for 200 mg/kg BPA and for E2 was similar (5.3 and 6.1, respectively). However, by 72 h, these responses had divergedthe 200 mg/kg BPA CC3 expression had returned to control level, while the response to E2 had amplified to >500-fold above control. As is evident from Figure 3, the C3 responses at 4 h occurred at the same dose levels (200800 mg/kg BPA) as the responses seen for PR and lipocalinwith the exception of the 2.5-fold increase in C3 only seen for 20 mg/kg BPA. In conclusion, the early imbibition response of the uterus to 200800 mg/kg BPA at 4 h is followed by the trophic response seen at 72 h (400800 mg/kg). Increases in the expression of uterine PR, lipocalin, and C3 also occur at 4 and 72 h after the same doses of BPA producing the physiological changes. The only exception to this is a small increase in C3 expression for 20 mg/kg BPA at 4 hthe biological significance of which is uncertain.
The changes in uterine PR protein level and peroxidase activity reported by Gould et al. (1998) for non-uterotrophic doses of BPA were all less than 2-fold (Table 1)the marginally greater activities seen for 100 and 150 mg/kg BPA are probably associated with the start of the uterotrophic effect. Consequently, there is a level of consistency between the data of Gould et al. (1998)
and the present datai.e., minor (<2-fold) fluctuations in gene expressions were observed in both studies.
The effects of low doses of endocrine disrupting compounds are highly controversial (Brucker-Davis et al., 2001; Melick et al., 2002
), particularly with respect to BPA where changes have proved difficult to replicate, for example prostate weight increases observed at 2 and 20 µg/kg BPA (Ashby et al., 1999
, 2004
; Cagen et al., 1999
; Nagel et al., 1997
). The use of microarray technology to identify novel genes differentially expressed by endocrine disrupting compounds, and real-time RT-PCR to quantify gene expression, has given rise to an increasing number of markers for endocrine disrupting activity (e.g., Naciff et al., 2002
; Rockett et al., 2002
; Watanabe et al., 2002
; Waters et al., 2001
). This technology has not been used extensively so far to identify genes which may be expressed at low dose levels, although Naciff et al. (2002)
showed that BPA below 50 mg/kg had no effect on estrogen-sensitive genes in the developing female reproductive system of the rat. Lobenhoffer et al. (2004)
have recently suggested that identification of a NOTEL (no transcriptional effect level) for compounds would be useful to identify doses below which transcriptional (and therefore toxicological or pharmacological) effects do not occur. They used this approach to demonstrate that only doses of E2 which are physiologically relevant, and above, were capable of inducing a transcriptional response.
In the present study we have attempted to determine whether changes in estrogen responsive genes occur at levels of BPA below those capable of inducing a uterotrophic response. We extended the dose levels from 800 mg to 2 µg/kg BPA in order to provide sufficient groups to detect low dose effects. Hunt and Bowman (2004) described a model for detecting low dose effects and stress the necessity for including sufficient groups. We also replicated the study to demonstrate reproducibility of the response. With the exception of changes in C3 at 20 mg/kg BPA, uterine growth and gene expression were observed at the same dose levels. Gene expression changes were of greater magnitude than the uterotrophic response, but there was no evidence of gene expression changes at low doses (below 2 mg/kg) in this study. Therefore, in the present study, the NOEL for uterotrophic activity for BPA coincided with the NOTEL for uterine transcriptional activity.
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NOTES |
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1 To whom correspondence should be addressed. Fax: (44) (0) 1625 590249. E-mail: Jenny.Odum{at}Syngenta.com
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