Institute of Environmental Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Received January 19, 2004; accepted March 26, 2004
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ABSTRACT |
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Key Words: Ah receptor; 2,3,7,8- TCDD; exonuclease III; S1 nuclase; real-time PCR.
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INTRODUCTION |
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Numerous analytical techniques have been developed to detect and quantify the AhR agonists in environmental, biological, and food samples. High-resolution gas chromatography/mass spectrometry (HR GC/MS) is the gold standard for HAH analysis with accurate and specific measurement (Clement, 1991; Stephens et al., 1992
). But the procedures are extremely costly and time consuming and require sophisticated equipment, which will not adapt for large numbers of samples to be screened. Additionally, this approach is unable to assess chemicals interactions and will not allow for the identification of other novel AhR agonists except HAHs (Safe, 1990
; Van den Berg et al., 1998
).
Currently, numerous AhR-based bioassays for identification and detection of AhR ligands have been developed in vitro (El-fouly et al., 1994; Garrison et al., 1996; Murk et al., 1996
; Postlind et al., 1993
; Wheelock et al., 1996
). Whole cell systems, such as the chemical-activated luciferase gene expression (CALUX) (Garrison et al., 1996
; Zhang et al., 2002
) and ethoxyresolufin-O-deethylase (EROD) activity (Giesy et al., 1994; Kennedy et al., 1993; Safe, 1990
) are sometimes represented as the next best system when compared with whole-body or in vivo systems. However, cell systems can be affected by the toxic chemical itself during the assay, thus causing confusing problems in the assay. Incorporation of metabolism in cell systems with uncertain consequences prolongs assay complexity and time. Thus, these drawbacks limit the utility of cell systems for screening purposes. Most cell-free bioassays require radioactivity, such as the gel retardation of AhR binding (GRAB) assay (Denison and Yao, 1991
; Heath-Pagliuso et al., 1998
;), or antibody of AhR or ligand (Santostefano et al., 1992
; Wheelock, 1996
), which are unfeasible for some laboratories.
Here a cell-free bioanalysis method, Exonuclease Protection Mediated PCR (EPM-PCR) bioassay, was established for detection of AhR ligands, based on the binding of the ligand:AhR complex to the specific DNA. EPM-PCR can provide indirect detection of ligands by quantification of the specific AhR-binding DNA, with no need for any DNA labeling and sophisticated equipments. This new bioassay not only has the higher sensitivity and specificity, but it is rapid and easy to perform.
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MATERIALS AND METHODS |
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Preparation of cytosol. Male Sprague-Dawley rats (200250 g) were exposed to 12 h of light and 12 h of dark daily and were allowed food and water. Treatment of animals was a standard operating procedure referring to the humane handling program of the lab of our university. Hepatic cytosol was prepared at 4°C in HEDGK buffer (25 mM Hepes, pH 7.5, 1 mM EDTA, 1 mM DTT, 10% (v/v) glycerol, and 80 mM KCl) according to Denison et al. (1986) and stored at 80°C until use. Protein concentrations were measured by the method of Bradford (1976)
using bovine serum albumin as the standard and adjusted to 1015 mg/ml diluted in HEDGK buffer.
Preparation of dioxin probe. The plasmid pAlterMax (Sigma Co.) was amplified with primers F1 (5'-CTCTTCTCACGCAACTCCGTGTAACAAGGGTGAACAC-3') and R1 (5'-CGGAGTTGCGTGAGAAGAGCGTTGATATATCCCAATG-3') to generate 285 bp-length PCR products containing two AhR binding sites (dioxin-responsive elements, DREs) on both ends. The underlined sequences corresponded to the AhR binding site of mouse DRE3 (Denison et al., 1988). PCR products were isolated using 2% agarose gel electrophoresis and purified to be referred to as dioxin probe. Probe sequence was identified by sequencing, quantified at OD260nm, and serially diluted every 10-fold at a range of 1 x 102 to 1 x 108 copies to be used in real-time PCR to supply the standard.
Preparation of TCDD:AhR:DRE complex. SD rat hepatic cytosol was incubated with DMSO (20 µl/ml), different concentrations of TCDD (0.01 pM10 nM) or other AhR ligands (each of 10 µM), for 2 h at 20°, then mixed with poly(dI/dC) (1.0 µg) and incubated for 15 min at 20°, followed by the addition of
0.5 µg DREs probe and further incubation for 15 min. Every indicated chemical was diluted in DMSO solution.
EMP-PCR bioassay. The 10 µl aliquots of binding reaction mixture described above were mixed with 40 µl of 1.25x ExoIII buffer, and preincubated for 5 min at 30°C, followed by addition of 100 U ExoIII and further incubation for 10 min. Next, 5 µl of ExoIII-treated aliquots was added to 15 µl S1 mix (200 µl mix containing 40 mM kAc (potassium acetate), pH 4.6, 340 mM NaCl, 1.35 mM ZnSO4, 0.8% glycerol, and 60 U S1 nuclease) to incubate for 30 min at room temperature. After the termination of digestion by addition of 2 µl S1 stop buffer (0.3 M tris-base, 0.05 M EDTA) and incubation for 10 min at 70°C to inactivate the enzyme, the sample of 10 nM TCDD was subjected to PCR with primers (F1, R1) or (F2, R2). Primer F2 (5'-CATTTCAGTCAGTTGCTC-3') and R2 (5'-CGTCTTTCATTGCCATAC-3') were designed to amplify the residual DNA with less primer dimers and nonspecific products. The conditions of PCR were an initial denaturation at 94°C for 5 min, followed by 30 cycles at 94°C for 45 s, 51°C for 45 s, and 72°C for 45 s, at last extended for 8 min. The target DNA products were identified by comparison to 100-bp DNA ladder on 2% agarose electrophoresis. For primers (F1, R1), the annealing temperature of PCR was 60°C.
Real-time quantitative PCR. Real-time PCR amplification was carried out in a 20-µl volume with 2-µl LightCycler DNA Mastermix (Roche Molecular Biochemicals, Mannheim, Germany) containing Taq-polymerase, dNTPs (with dUTP instead of dTTP), PCR buffer, and the Sybr green I. MgCl2 and primer concentrations were optimized for highest signal intensity and lower background. Here the concentration of MgCl2 was 3 mM, and primer concentration was 0.75 µM. Different concentrations of 2-µl TCDD-treated samples were used as the templates. PCR were run in the LightCycler amplification and detection system (Roche Diagnostics). Protocol was done by 0 s denaturation at 95°C, 5 s annealing at 51°C,and 10 s elongation at 72°C for 40 cycles. Fluorescence was detected at the end of every 72°C extensive phase. Melting curve analysis was applied to all end PCR products after cycling protocol. The melting step consisted of denaturation at 95°C, cooling to 65°C for 10 s, then ramping to 90°C at 0.2°C /s, monitoring fluorescence continuously.
To determine the absolute copy numbers of the bound DNA in samples, ten-fold dilution series of dioxin probe (1 x 102 to 1 x 108 copies ) were used in the real-time PCR. Threshold cycle (CT) values are defined as the cycle at which Sybr green I fluorescence increases significantly above the background as a result of amplicon formation and calculated by the LightcyclerTM software program version 3.5 (Roche Diagnostics). From a dilution series of a standard with known concentration of target sequences, a calibration curve is created (CT vs. logarithm of the starting concentration). Each standard sample was run in triplicates, and the average amplification efficiency was calculated from the slope of the calibration curves (PCR efficiency = 101/slope).
CALUX assay. The CALUX assay utilized recombinant human hepatoma (HepG2) cells that we previously generated, and the protocol was described by Zhang (2002). In brief, HepG2 cells were engineered to contain a stably integrated DRE-driven luciferase reporter gene plasmid based on the vector pGL3. The recombinant HepG2 cells were incubated with DMSO (0.5%) or TCDD (0.1 pM10 nM)) for 24 h at 37°C. The cells were lysed by luciferase lysis reagent (Promega), and luciferase activity was measured using a Lumate LB 9570 luminometer with luciferase assay reagent (Promega). The concentration of protein was detected by Bio-Rad method. Luciferase activity was expressed as relative light unit (RLU) per milligram protein.
Every concentration of ligands was determined using EPM-PCR and CALUX assay at least three times, and each value represents the mean ± standard deviations (SD). Coefficients of variation (CV) were calculated by (SD/mean x 100%).
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RESULTS |
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Calibration curves of three runs were obtained using different copies ranging from 102 to 108 copies of the dioxin probe as the templates in real-time PCR (Fig. A was shown in Supplementary Data). Primers (F2, R2) were chosen here. They all showed a linear relation (r = 0.99) between the log of template copy number ([log copy number]) and CT over the range of DNA copies examined. The PCR efficiencies ranged from 1.7 to 1.8. The samples of ExoIII/S1 digestion were quantified by real-time PCR (Figs. B and C were shown in the Supplementary Data). The results demonstrated CT values decreased with the increasing concentration of TCDD. Increasing the 10-fold TCDD reduced the CT value by 4. Melting-curve analysis revealed that PCR amplified a single product. On the agarose gel electrophoresis, the end product of PCR was a single band of the predicted size (182 bp). Corresponding to the data above, the linear relationship of TCDD concentrations to bound dioxin probe copies was obtained (Fig. 3). It showed the ability of a chemical to stimulate AhR transformation and DNA binding in vitro was dose dependent. The minimal detection limit (MDL), whose fluorescent signal is 10-fold higher than SD of the mean baseline emission, is 1 x 1014 M TCDD. We also tested the high concentration (20 nM, 50 nM, and100 nM) of TCDD with this system. The CT values had no significant difference (p < 0.05). Thus we concluded that the maximal amount of bound DNA was produced at 10 nM TCDD, and the concentration of half-maximal respond (EC50) was estimated to be 3 x 1012 M.
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DISCUSSION |
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The standard protocol of the exonuclease protection assay was described by Wu (1985). It detected protected DNA (containing the nonspecific nucleic acid) on the agarose electrophoresis directly after ExoIII digestion. This assay is unfeasible for detecting the trace protein-binding DNA, due to low sensitivity, and is incapable of distinguishing the specific and nonspecific DNA. PCR is a good technique for amplifying the trace DNA, but it is not suitable for classic exonuclease protection assay. The high frequency of false-positive results is achieved because the end-blunt DNA, digested to 7090% of completion by ExoIII, remains a short double strand even after extensive degradation (Richardson et al., 1964
). The single-stranded oligonucleotides produced by ExoIII can be amplified by PCR. With ExoIII digestion alone, the results had no significant difference between the negative control and indicated chemicals when PCR was performed. Even if the digestion time was extended to 60 min, the results were similar (data not shown). In this case, the addition of S1 nuclease resolved the problem of high background value. The free DNA treated with ExoIII was deleted quickly by S1 nuclease and was not be amplified by PCR. The utilization of PCR improved the sensitivity of EPM-PCR and made it feasible to detect the trace dioxins.
To clarify the specificity of EPM-PCR bioassay, the mutated DNA probe (without DRE) replaced the indicated dioxin probe in EPM-PCR, and no signal was observed. Poly(dI/dC) was preincubated with the samples to eliminate nonspecific protein-binding DNA in the subsequent reaction steps. In fact, no differences on the result of PCR with and without the addition of poly(dI/dC) in the reaction mixture were detected. It may be because, even if the nonspecific protected DNA exists, it cannot be detected in the present system by the primers used. In fact, EPM-PCR is applicable to a variety of cell lines or species. The crude protein extract also contains other proteins that may preferentially bind to the DNA probe input. If there was such binding, specific protected DNA was confirmed by melting curve analysis of PCR product. Taqman and molecular beacon quantitative PCR with higher specificity are good alternatives to measure the AhR-binding dioxin probe. EPM-PCR assay avoids the loss of TCDD:AhR-binding DNA and interference of the free or nonspecific DNA by eliminating, not separating, the free DNA from bound DNA, which improved the specificity.
Besides TCDD, EPM-PCR assay was also used to examine the ability of other three ligands to directly stimulate AhR transformation and DNA binding in vitro (data not shown). Dose-response curves demonstrated the rank order in potency was TCDD > TCDF > PCB > BA, which was consistent with their toxic equivalency factors (TEFs).
By applying of ExoIII and S1 nuclease digestion in the AhR-binding DNA bioassay, EPM-PCR provides sensitive and specific detection and quantification of AhR ligands. Compared with other existing methods for dioxin detection, EPM-PCR bioassay has advantages, such as nonradioactivity, sensitivity and simplicity. It is useful to screen the positive from large-scale of samples or measure novel AhR agonists. However, it doesn't provide structural information of the agonists like GC/MS analysis. So addition of the structure-based approach is necessary for identification of an unknown AhR agonist.
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SUPPLEMENTARY DATA |
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ACKNOWLEDGMENTS |
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NOTES |
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1 To whom correspondence should be addressed at Institute of Environmental Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province 430030, China. Fax: +862783657705. E-mail: shunqing{at}public.wh.hb.cn.
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