* Department of Biochemistry and Molecular Biology, National Food Safety and Toxicology Center, Center for Integrative Toxicology, and Department of Pharmacology and Toxicology Michigan State University, East Lansing, Michigan, 48824
1 To whom correspondence should be addressed at Michigan State University, Department of Biochemistry and Molecular Biology, 224 Biochemistry Building, Wilson Road, East Lansing, MI 488241319. Fax: (517) 353-9334. E-mail: tzachare{at}msu.edu.
Received March 17, 2005; accepted May 2, 2005
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ABSTRACT |
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Key Words: benzo[a]pyrene; aryl hydrocarbon receptor; Hepa-1c1c7.
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INTRODUCTION |
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Hepa-1c1c7 mutants were selected and isolated based on their resistance to benzo[a]pyrene (B[a]P) toxicity and compromised AHH induction (Bartholomew et al., 1975; Hankinson, 1979
; Thompson et al., 1974
). Three isolated complementation groups (A, B, and C) were identified and used to investigate the mechanism of action of the aryl hydrocarbon receptor (AhR) (Van Gurp and Hankinson, 1984
). C1 mutants (Group A) are characterized by a deficiency in Cyp1a1 activity as a result of a premature stop codon located after Asn 414, resulting in a loss of a C-terminal heme-binding site necessary for enzyme activity (Kimura et al., 1987
). C12 mutants (Group B) express only 3% of the AHH activity of wild-type cells (Van Gurp and Hankinson, 1984
) due to compromised AhR expression, possibly from a defective factor involved in chromatin remodeling (Hankinson et al., 1985
; Zhang et al., 1996
). Hepa-1c1c7 c4 (Group C) mutant cells are devoid of any AHH activity, due to a G326D point mutation in the ARNT (aryl hydrocarbon receptor nuclear translocator) coding region, which causes a marked reduction in DRE sequence binding by the AhR/ARNT complex (Numayama-Tsuruta et al., 1997
).
Studies using Hepa-1c1c7 wild-type and mutant cells have significantly contributed to the elucidation of the mechanism of AhR-mediated gene expression (Boverhof et al., 2005; Hoffman et al., 1991
; Probst et al., 1993
; Reyes et al., 1992
), including the identification of responsive detoxification genes (Liu et al., 1994
). However, isogenicity of loci beyond Cyp1a1 is not expected, given the selection method and criteria used for mutant cell line isolation. Despite their use in numerous studies (Jeong and Lee, 1998
; Merchant et al., 1992
; Miranda et al., 2000
; Pollenz, 1996
; Seubert et al., 2002
; Zacharewski et al., 1994
), only one has examined differences in constitutive gene expression between Hepa-1c1c7 wild-type and mutant cells using differential display PCR in wild-type and the related but independently derived ARNT-deficient Hepa-1 cell line derivative, BPRc1 (Seidel and Denison, 1999
). Our study uses cDNA microarrays to further examine differences in basal gene expression in mutant and wild-type cells with regards to variant morphology and growth rates. Differences in basal gene expression were also linked to cellular physiological observations to identify mechanistic networks that may be affected in the mutant hepatoma cell lines. These data indicate that differences between wild-type and mutant cells may not be attributed solely to differences in AhR/ARNT functionality and Cyp1a1 inducibility.
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MATERIALS AND METHODS |
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Images were captured with Nikon Eclipse TE300 microscope and GelExpert software (San Carlos, CA). Measurements were conducted using ImageJ v132j (NIH, USA) and significance determined using SAS version 8.0. One-way ANOVA analysis followed by Dunnett's t-tests were performed on measurements where = 0.05.
Cell growth rate assessments.
For MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assays, 10,000 cells were seeded in a 96-well tissue-culture plate (Corning, MI) in 5% FBS and antibiotic supplemented media. Thaizylol blue (Sigma, St. Louis, MO) was added to cells, incubated for 3 h at 37.5°C and 5% CO2, and absorbance at 595 nm was determined using Softmax software (Molecular Devices, Sunnyvale, CA) at 3, 6, 12, 24, 36, and 48 h on an Emax 96-well microplate reader (Molecular Devices, Sunnyvale, CA). Experiments were completed in quadruplicate. Relative viability was standardized to the blank wells containing media only.
For direct cell counts, 3 x 105 cells were seeded in T25 culture flasks (Corning, MI) in 5% FBS and antibiotic supplemented media. Cells were incubated at 37.5°C and 5% CO2 for 6, 12, 24, and 48 h. Cells were then trypsinized and subjected to manual counting, in duplicate, using a hemocytometer (Hausser Scientific Co., Horsham, PA). Experiments were completed in quadruplicate.
Significance was determined using SAS v.8.0. Two-way ANOVA analysis followed by Tukey's HSD post hoc test was performed on MTT assay data where = 0.05. For the direct cell counts, a repeated measures ANOVA was performed followed by a Tukey's HSD post hoc test where
= 0.05. Comparisons were made between each mutant and wild-type cell line at each time point.
Microarray processing.
cDNA arrays containing 6376 features (representing 4858 unique genes based on mouse Unigene build 138), were spotted in duplicate, on epoxy-coated glass slides (SCHOTT Nexterion, Germany) using an Omnigrid arrayer (GeneMachines, San Carlos, CA) equipped with 16 (4 x 4) Chipmaker 2 pins (Telechem International Inc., Sunnyvale, CA) at the Genomics Technology Support Facility (http://genomics.msu.edu). Three biological replicates for each cell line were examined using a 6-slide independent reference design that incorporated dye-swap hybridizations (Fig. 1) for a total of 18 microarray hybridizations analyzed. Clones for the array were initially selected for the examination of testis-specific genes, and the library has since been expanded to include genes known to be responsive to estrogens, androgens, and dioxin. Selected clones were obtained from EPAMAC sequence verified mouse clones (Rockett and Dix, 1999), Research Genetics IMAGE clones, and the National Institute of Aging 15K mouse clone set.
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Quantitative RT-PCR.
A sample of total RNA isolated from each replicate was set aside for verification using SYBR® Green-based quantitative real-time PCR (QRT-PCR). Briefly, 1 µg of RNA was primed by random hexamers (Roche, Switzerland) and reverse transcribed using Superscript II in a 20-µl reaction as described. From a 5-fold dilution of this cDNA solution, 3 µl was used in a 30-µl PCR reaction containing 1x SYBR Green PCR buffer, 3 mM MgCl2, 0.33 mM dNTPs, 0.5 IU AmpliTaq Gold (Applied Biosystems, Foster City, CA) and 0.15 mM forward and reverse primer. All primers were designed by submitting RefSeq sequences to Primer3 (http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi) to identify an approximately 125bp amplicon (Table 1). PCR amplification was conducted in 96-well MicroAmp Optical plates (Applied Biosystems) on an Applied Biosystems PRISM 7000 Sequence Detection System under the following conditions: 10 min denaturation and enzyme activation at 95°C, followed by 40 cycles of 95°C for 15 s and 60°C for 1 min. A 30-min dissociation protocol, after amplification, was conducted to assess primer specificity and product uniformity. Each plate contained duplicate standards of purified PCR product of known template concentration over the range of eight orders of magnitude to generate a log template concentration standard curve. No template controls (NTC) were included on each plate where samples with a Ct value within 2 SD of the mean Ct values for the NTCs were considered below the limits of detection. Plots were visualized and thresholds determined using ABI Prism 7000 SDS Software (Applied Biosystems, Foster City, CA). Results were normalized to beta-actin to control for differences in RNA loading, quality and cDNA synthesis. Statistical significance of expression differences between wild-type line and mutant lines were assessed using a standard one-way ANOVA followed by Tukey's HSD post hoc analysis using SAS version 8.0 (Cary, NC).
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RESULTS |
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Microarray Quality Assessment and Statistical Analysis
All arrays within this study were compared to a historical data set of established high-quality arrays. Background signal intensity, feature signal intensity, feature versus background signal intensity ratios, number of features with background intensities greater than the feature intensity for each array, and relationships between feature and background signal intensities were examined. Comparisons indicate that both background and signal intensities were higher than the historical data set; however, all arrays that were used for further analysis met the quality standard requirements (Burgoon et al., submitted).
Basal Gene Expression Differences between Hepa-1c1c7 and Wild-Type and Mutant Cell Lines
Statistical analysis using a model-based t-statistic test (t-value > |2.97|) identified a total of 946 active features (spots on an array) across c1, c4, and c12 mutant cell lines when compared to wild-type cells, 723 of which are unique and represent 499 unique LocusLink annotated genes. The purpose of the t-statistic is to rank and prioritize clones to be initially examined and a cut-off selected to generate a manageable clone list for additional functional investigation.
C1 mutants exhibited the largest number of differentially expressed basal level of genes (312) when compared to wild-type cells, followed by c4 (220) and c12 (134) cells, respectively (Fig. 4A). A total of 30 genes exhibited differential basal levels of expression in all mutant lines versus the wild type, while 63, 35, and 9 genes demonstrated differential basal levels in sets of two cell lines, c1 and c4 lines, c1 and c12 lines, and c4 and c12 lines, respectively, relative to wild-type (Fig. 4B).
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Agglomerative hierarchical clustering revealed two main clusters: (1) WT with c12 and (2) c1 with c4 (data not shown). This result correlates with the number of basal gene expression differences observed where the c12 line exhibited the least number of differences, while both c1 and c4 lines each exhibited approximately twice the number of differences of the c12 line (Fig. 4).
Functional roles for constitutively expressed genes identified through Gene Ontology annotations (www.geneontology.org) and complemented with literature reports suggest that Hepa-1c1c7 mutant cell lines likely carry multiple mutations affecting a number of divergent pathways. Differentially regulated genes were organized into biological processes including DNA replication, adhesion, and carbohydrate metabolism (data not shown). Genes involved in cytoskeletal organization, bioenergetics, and cell cycle were associated with the morphological, mitochondrial activity, and proliferative rate differences observed between the wild-type and mutant lines (Tables 35).
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DISCUSSION |
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To further assess differences in constitutive gene expression between wild-type and mutant cells, functional annotation for differentially expressed genes was extracted from Gene Ontology, LocusLink, and the published literature. Gene expression differences were classified into mechanistic pathways and associated with cellular characteristics such as morphology and proliferation. Overall, the differences in constitutive gene expression were consistent with cellular characteristics that defined a mutant cell line.
Cellular Morphology
Differences in morphology observed between wild-type and mutant lines were reflected in differentially expressed genes involved in cytoskeletal organization. Although c4 and c12 mutants exhibited fewer changes in basal expression of cytoskeletal organization genes, they are morphologically distinct from the wild-type cells with a narrower morphology and longer processes. All differentially regulated cytoskeletal organization genes in c4 cells had higher basal expression compared to wild-type, including vimentin (Vim), which is involved in lymphocyte dendrite extension (Sumoza-Toledo and Santos-Argumedo, 2004). Intermediate filament and microtubule genes, represented by integrin beta 4 binding protein (Itgb4bp), microtubule-actin crosslinking factor 1 (Macf1) and alpha 4 tubulin (Tuba4), may also be involved, as orientation of these components facilitates process extension (Sumoza-Toledo and Santos-Argumedo, 2004
) and suggests a coordinated effort of these genes to contribute to c4 elongation.
In c12 cells, increased basal expression of select genes may also lead to increased actin filament formation. Higher basal expression of phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a) generates the secondary messenger phosphoinositol 4-phosphate, PI4P, which is essential for normal actin organization through Cdc42 GTPases (Wild et al., 2004) and is representative of phosphoinositide regulated pathways (reviewed in Lemmon, 2003
). However, this activity may be counteracted by the lower basal expression of erythrocyte protein band 4.1-like 5 (Epb4.1l5). Epb4.1l5 belongs to a family of proteins which interact with the actin filament (Takeuchi et al., 1994
) through a phosphoinositide binding regulatory domain for phosphoinositol 4,5-bisphosphate (PI4,5-P2), a product of PI4P phosphorylation (reviewed in Lemmon, 2003
). Also increased basally in c12 cells is vinculin (Vcl), a focal adhesion protein which has been shown to be up-regulated upon actin disruption (Quinlan, 2004
). Although there are fewer cytoskeletal gene expression changes in c4 and c12 cells, overall they are consistent with the apparent stretched morphology compared to the wild-type population.
Despite c1 cells exhibiting the most gene expression changes associated with cell morphometry, this was not reflected in a significant difference in its size, shape, or number of processes compared to wild type. These changes in basal expression may be offset by other genes resulting, in an overall absence of net change to cellular morphology. For example, actin is a key player in determining cellular morphology and undergoes constant polymerization and redistribution. Microarray analysis shows lower basal expression of annexins (Anxa3 and Anxa4) in c1 mutants compared to wild-type. Annexins are regulators of actin dynamics (Hayes et al., 2004), and their decreased basal expression indicates depolymerization of actin filaments. Dystroglycan 1 (Dag1), a scaffold for MEK/ERK signaling to maintain actin levels (Natalicchio et al., 2004
; Spence et al., 2004
) and Septin 2 (Sept2), a cytoskeletal component whose function is associated with actin-based structures during cytokinesis (Kinoshita et al., 1997
), are also found to have lower basal expression in c1 cells. The activity of these genes may be compensated by the greater basal expression of annexin 7 (Anxa7) (Hayes et al., 2004
) and cortactin (Cttn), which are involved in actin-mediated motility (Patel et al., 1998
), and Rac GTPase-activating protein 1 (Racgap1), responsible for actin-based spreading (Wells et al., 2004
), in an effort to maintain actin levels. As a result, the cumulative balance may not be shifted and, thus, may have little influence on overall morphology. This is consistent with the observation that the c1 cells are morphologically similar to wild-type cells. However, regulation of actin is also largely dependent on ATP hydrolysis and PIP2 signaling (reviewed in Gungabissoon and Bamburg, 2003
) that cannot be detected by microarrays.
Mitochondrial Activity
The MTT assay evaluates mitochondrial activity through dehydrogenase conversion of soluble thaizylol blue to an insoluble dye for colorimetric analysis. Both c4 and c12 cells exhibited higher mitochondrial activity when compared to the wild type. Examination of the differentially regulated genes indicates that c4 cells exhibit the largest number of gene expression changes associated with mitochondrial activity. ATP synthase subunits (Atp5g1 and Atp5o) (Chen et al., 1995; Li et al., 2001
), cytochrome c oxidase subunit (Cox6b) (Ohtsu et al., 2001
), and NADH dehydrogenase Fe-S subunits (Ndufs2 and Ndufs4) (Loeffen et al., 1998
; Papa et al., 2002
) are all components of the mitochondrial electron transport chain and show higher basal expression in the c4 cells, consistent with increased basal mitochondrial activity. However, only Cox8a, a subunit of cytochrome c oxidase (Huttemann et al., 2003
), exhibited a significant increase in basal expression that could be associated with the increased mitochondrial activity of the c12 cell line. This indicates the augmented activity may involve other genes not represented on the array or by other nontranscriptionally mediated factors, such as changes in enzymatic activity due to posttranslational signaling events. In addition, it could also reflect the incomplete status of the available gene annotation.
In the c1 mutants, Cox6b exhibited lower basal expression, in agreement with the lower MTT activity. However, the higher basal expression of NADH dehydrogenase subunits (Ndufa1 and Ndufv1) (Au et al., 1999; Schuelke et al., 2002
) suggests higher mitochondrial activity should have been observed.
Expression differences in mitochondrial transcripts may address some of the differences in mitochondrial activity detected by the MTT assay, but the activity itself also correlated with the observed proliferative rates. Thus, changes in mitochondrial gene expression in mutant cells may be attributed to different energy needs related to their proliferative rate. Additional mechanisms may also be involved with the differences in basal mitochondrial activity, as transcript levels do not reflect changes in protein levels or enzymatic activity.
Proliferation
Despite exhibiting a slower proliferative rate, numerous proliferative genes exhibited higher basal expression in c1 cells, including insulin-like growth factor 1 (Igf1) and thymopoietin (Tmpo). Igf1 is a well-known mediator of cell proliferation through receptor signaling (reviewed in Furstenberger and Senn, 2002), while Tmpo may have a role in cell cycle control, as its transcripts are more abundant in cancerous cell lines compared to tissues in a slower proliferative state (Weber et al., 1999
). Ornithine decarboxylase (Odc), involved in the synthesis of polyamines which support cell proliferation (reviewed in Luk and Casero, 1987
), exhibited increased basal expression, while the lower transcript levels of glypican 1 (Gpc1), a membrane-anchored polyamine receptor, restrict polyamine uptake, consistent with the ligand-mediated feedback mechanism associated with this receptor (Belting et al., 2003
). Collectively, this suggests that the c1 mutants would have a higher proliferative rate in comparison to the wild-type line. Furthermore, lower basal expression of cyclin-dependent kinase inhibitor 2d (Cdkn2i), an inhibitor of cell cycle kinases (Hirai et al., 1995
), should also facilitate proliferation. However, the decrease in basal transcripts of syndecan 1 (Sdc1), a growth hormone receptor involved with adhesion, is consistent with the decrease proliferation observed with the c1 mutants (Maeda et al., 2004
) in addition to its role in tumor invasion (Langford et al., 2005
). The lower proliferative rate of c1 cells suggests the lower basal expression of Sdc1 may dominate, while changes in gene expression supportive of proliferation serve a compensatory role in an attempt to reestablish wild-type proliferation rates. These inconsistencies between changes in gene expression and functional assays illustrate the importance of phenotypic anchoring in the interpretation of gene expression data.
Numerous basal expression differences in c4 cells were found to be associated with proliferation. Although c4 cell proliferation was not found to be significantly different from WT, the data trend indicates that significance may be detected beyond 48 h. Studies beyond 48 h were not conducted, as cell cultures approached confluency which may confound the interpretation due to different proliferation kinetics. Higher transcript levels of cyclin D2 (Ccnd2) and cyclin-dependent kinase 4 (Cdk4) are required for the G1 S transition (Ando et al., 1993
; Ando and Griffin, 1995
), suggesting that the cells are positively regulated for growth. RanGTPase hydrolytic activity is essential for DNA synthesis, and its activity is mediated by RAN GTPase activating protein 1 (Rangap1), which is subsequently promoted through RAN-binding protein 1 (Ranbp1) interaction (Guarguaglini et al., 2000
). Higher basal expression of Rangap1 and Ranbp1 may be factors regulating the elevated proliferation observed in the c4 mutants.
Other increases in basal transcript levels appear to limit proliferation, such as ornithine decarboxylase antizyme (Oaz1), which represses polyamine synthesis by promoting Odc degradation (Schipper et al., 2004), growth arrest specific 1 (Gas1), an inducible proliferation inhibitor (Mellstrom et al., 2002
), and ubiquitin-conjugating enzyme E2C (Ube2c), which degrades cyclin B1 in plants (Criqui et al., 2002
). Although these anti-proliferative signals are present, the overall pro-proliferative effects prevail.
General Conclusion
Our study demonstrates that numerous genes in the B[a]P-resistant mutant cell lines derived from the Hepa-1c1c7 line are differentially regulated at the basal level in comparison to the wild-type parent. We have observed changes in gene expression that may contribute to differences in morphology, mitochondrial activity, and cell proliferation rates, suggesting that B[a]P selection introduced mutations in addition to the well-characterized affects on Cyp1a1, AhR, and ARNT. However, the results are limited by the lack of an array with cDNA representative of all mouse genes, the lack of confirmation of increases or decreases in basal protein expression and/or enzymatic activity, and the realization that the available functional annotation is limited and likely biased. Nevertheless, these results indicate that conclusions regarding reported differences between wild-type and mutant cell lines may be partially attributed to factors other than mutations to Cyp1a1, AhR, and ARNT alone.
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SUPPLEMENTARY DATA |
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ACKNOWLEDGMENTS |
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Conflict of interest: none declared.
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