This book contributes a comprehensive collection of experimental protocols optimized for the investigation of the articular cartilage.
The adult articular cartilage is characterized by a very low ratio between cells and the proteoglycan-rich extracellular matrix. Because of this peculiar composition, the investigator is confronted with technological hurdles that require thorough adaptations of standard procedures. Putting together optimized published protocols that are scattered through the literature, often in a succinct form, is a frustrating and often disappointing endeavour. This book responds to the need for a comprehensive technological reference for the study of the articular cartilage.
The first volume covers the cellular, molecular and biochemistry tools for in vitro studies. Optimized protocols are described for RNA extraction, proteomics, transfection and transduction methods, specialized culture systems and classical assays, such as those for the proteoglycan content of cartilage explants. The second volume describes techniques for in vivo studies, including the generation of transgenic mice, histochemistry, immunohistochemistry, in situ hybridization, biochemical tests, joint surface imaging, histomorphometry and the biomechanical evaluation of normal and pathological cartilage.
The book is complete and detailed. Eliminating several redundancies would improve this book in further editions. In addition, some of the techniques described do not require special adaptations for cartilage. For instance, methods for the construction of adeno- or retroviruses and the generation of transgenic mice for studies on cartilage do not differ significantly from the standard procedures, and the protocols described are probably insufficient to enable the novice to perform these experiments.
This book represents a useful reference for methods in cartilage biology/tissue engineering laboratories as well as in clinical departments engaged in osteoarthritis research. It will be invaluable for the young student, but also very useful to the experienced investigator.
The author has declared no conflicts of interest.
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