Corrigendum

Online Release 11.2, October 2002

Print Volume 11, January 2003

Pages 99107: Teng PI, Dichiara MR, Kömüves LG, Abe K, Quertermous T, and Topper JN. "Inducible and selective transgene expression in murine vascular endothelium" (http://physiolgenomics.physiology.org/cgi/content/full/11/2/99; doi:10.1152/physiolgenomics.00059.2002). In the METHODS section, in the paragraph "Generation of transgenic mice", there is a typographical error in the sequence of the PCR reverse primer for the Tie2-rTA transgene: there is a T nucleotide missing in the fifth triplet. This should read "The following primers were used for amplification of the Tie2-rTA sequence: forward, 5'-GCT CGC ATG GTC CAC TCG-3', and reverse, 5'-GCA AAA GTG AGT ATG GTG CC-3', which resulted in a product size of 260 bp." Also, Teng et al. have since identified another Tie2 forward primer that is more reliable than the forward primer used previously (from the 2002 article). This new primer is based on the Tie2 promoter sequence, and it is available from Integrated DNA Technologies (ref. 6268503): 5'-CGA GTG GAC CAT GCG AGC -3'. In order to make our transgenic mice available to those seeking to elucidate the genetic interplay involving the endothelium, we have licensed our mice to the Jackson Laboratory, which is currently working on making the animals available for general distribution. For further information, please contact Peter I. Teng (E-mail: piteng{at}yahoo.com).





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