Departments of 1Biological Chemistry and 4Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel and 2Department of Medical Biochemistry and Genetics, Reynolds Building, Texas A & M University, College Station, TX 77843, USA 3These authors contributed equally to this work
5 To whom correspondence should be addressed. e-mail: joel.sussman{at}weizmann.ac.il
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Abstract |
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Keywords: carbonic anhydrase activity/Dunaliella salina/halophilic proteins/protein molecular adaptation/salt-tolerant protein
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Introduction |
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In addition to proteins from halophilic archaea, several enzymes of eubacterial (Deutch, 2002; Chantawannakul et al., 2003
) and eukaryotic origins (Ahmad et al., 2001
; Su and Lee, 2001
) were reported to exhibit considerable salt tolerance, i.e. they remain active over relatively broad salinity ranges, without showing obligatory salt dependence. Despite the great interest in the structural and mechanistic aspects that underlie protein salt tolerance, these proteins have not been subjected yet to rigorous analyses.
A potential source of novel salt-tolerant proteins is the unicellular green alga Dunaliella salina that is capable of proliferating in salinities as varied as those prevailing in mesophilic all the way to halophilic habitats. Unlike extreme halophiles, the alga maintains a low intracellular ionic level over practically the entire range of salinities and attains osmotic balance by intracellular accumulation of glycerol (Avron, 1986). Consequently, intracellular Dunaliella proteins are not exposed to the environmental salinity, in contrast to surface proteins, which are expected to remain active over the broadly varying salinities sustaining algal growth.
A protein fulfilling this expectation is dCA I (Dunaliella carbonic anhydrase I, formerly designated Dca, accession number P54212), a salt-inducible 60 kDa protein consisting of two, 52% identical, tandemly arranged
-type carbonic anhydrase domains (Fisher et al., 1996
; Gokhman et al., 1999
). Carbonic anhydrases catalyze the reversible hydration of CO2 and perform essential functions in animals, plants, eukaryotic algae and eubacteria (Burnell, 2000
; Fukuzawa et al., 2000
; Kozliak et al., 2000
; Parkkila, 2000
). Its internally duplicated structure distinguishes dCA I from other, single-domain
-type carbonic anhydrases (EC 4.2.1.1) that are
30 kDa in size and it is the first internally duplicated
-type carbonic anhydrase to be analyzed enzymatically. The plasma membrane (PM) association of dCA I (Fisher et al., 1994
, 1996
) is attributed to a still unspecified post-translationally added anchor. Based on its function, salt inducibility and extracellular orientation, dCA I was proposed to play a role in enhancing the supply of CO2 to the cells, especially under CO2 limiting conditions such as high salinities (Fisher et al., 1994
). The expected salt tolerance of dCA I, as compared with the salt sensitivity typically exhibited by
-type carbonic anhydrases, was first supported by assays of a crude protein fraction from D.salina including dCA I (Fisher et al., 1996
).
That dCA I may belong to a larger set of salt-tolerant carbonic anhydrases in D.salina has recently been indicated by the identification of another extracellular protein consisting of a single -type carbonic anhydrase domain (Premkumar et al., 2003b
). Designated dCA II (Dunaliella carbonic anhydrase II), this enzyme is
50% identical in sequence with each of the dCA I domains, similarly enriched in acidic amino acid residues and exhibits comparable activity to dCA I (Premkumar et al., 2002
). Recently, two other cDNA sequences for carbonic anhydrases from Dunaliella differing from dCA I and dCA II were reported (AAP33144, AAO83593), but still need to be characterized on the protein level.
Aside from implying global stability in the presence of low to multimolar salt concentrations, the expected salt tolerance of dCA I is of special interest in view of the fact that, like other -type carbonic anhydrases, this enzyme is a Zn metalloprotein. The active-site Zn is tetrahedrally coordinated to three conserved His residues and a hydroxyl or water moiety (Liljas et al., 1972
; Stams and Christianson, 2000
). In the CO2 hydration reaction, the Zn-bound hydroxide reacts with CO2 to form bicarbonate, a step followed by the regeneration of the Zn-bound hydroxide by intermolecular proton transfer from Zn-bound water, that replaces the released bicarbonate, to a buffer molecule in the solution (Lindskog and Silverman, 2000
). Various anions, as well as sulfonamides and other inhibitors, interfere directly with the catalytic process by coordinating with the active-site Zn, thus replacing the catalytically essential, Zn-bound water/hydroxyl as well as the deep water (hydrogen bonded to Zn-bound water/hydroxyl and peptide nitrogen of Thr199) (Liljas et al., 1994
). For this reason,
-type carbonic anhydrases are usually inhibited by relatively low concentrations of halides and other salts.
The results of the analyses presented here establish the unique salt/anion tolerance of the enzyme, point to structural elements underlying these responses and provide an intriguing example for the type of enzyme structural modifications integral to the evolution of salt-tolerant organisms.
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Materials and methods |
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The Dunaliella salina strain and growth conditions were essentially as described elsewhere (Ben-Amotz and Avron, 1983; Sadka et al., 1989
; Fisher et al., 1994
). In addition to the standard components, the medium used for induction of dCA I contained 3.5 M NaCl, 50 mM Tricine-HCl, pH 8.5 and 25 mM Na bicarbonate. Cultures, usually started by inoculating cells from single algal colonies into liquid media, were grown to a density of
2x106 cells/ml and further propagated by diluting to (12)x105 cells/ml in fresh media.
Affinity chromatographic purification of dCA I from D.salina
Cells from a 30 l culture were harvested by centrifugation at 2000 g and lysed by re-suspension in 100 ml of 25 mM Tris-SO4, pH 7.5, 50 mM Na2SO4, 100 mM NaClO4, 5 mM -caproic acid, 1 mM benzamidine, 0.1% Triton X-100 (Equilibration Buffer 1). The suspension was centrifuged at 186 000 g for 60 min at 4°C and the supernatant, containing
500 mg of protein, was loaded on to a column (45x2.2 cm i.d.) of agarose-coupled p-aminomethylbenzenesulfonamide (PAMBS) (Sigma) (Whitney, 1974
) in Equilibration Buffer 1. After washing the column with 400 ml each of Equilibration Buffer 1, 0.2 M NaClO4 in the same buffer and a similar solution but without Triton X-100, dCA I was eluted with 0.5 M NaClO4, 0.1 M Na acetate, pH 5.6, 5 mM
-caproic acid, 1 mM benzamidine (Whitney, 1974
). Fractions with
95% pure dCA I were equilibrated with 25 mM Tris-SO4, pH 7.5, 50 mM Na2SO4, 5 mM
-caproic acid, 1 mM benzamidine and concentrated to
1.5 ml by using an Amicon spin column (30 kDa cut-off size). The total recovery of pure dCA I was 13 mg.
Preparation of a plasma membrane fraction and isolation of a 40 kDa proteolytic product of dCA I (p40)
A 25 l D.salina culture was grown and harvested as described above. The cells were re-suspended in 40 ml of iso-osmotic buffer containing 4.5 M glycerol, 5 mM -caproic acid, 1 mM benzamidine and 10 µM ZnSO4 and lysed by adding 160 ml of 10 mM Tris-MOPS [3-(N-morpholino)propanesulfonic acid], pH 7.5. The lysate was centrifuged at 8000 g for 10 min at 4°C and the supernatant was centrifuged at 200 000 g for 2 h at 4°C. The plasma membrane-enriched pellets (Azachi et al., 2002
) were resuspended in 10 ml of 10 mM Tris-MOPS, pH 7.5, 5 mM
-caproic acid, 1 mM benzamidine, 10 µM ZnSO4, 0.2% Triton X-100 (Suspension Buffer) and the suspensions were incubated on ice for 10 min and centrifuged at 200 000 g for 2 h at 4°C. The supernatant, containing 1525 mg of protein, was loaded on to a DEAE-Sephadex (A-25) column (30x1.8 cm i.d.), pre-equilibrated with Suspension Buffer and the column was washed with 250 ml of the same buffer and eluted with 200 ml of a 00.6 M NaCl linear gradient in the same buffer. Aliquots were assayed for carbonic anhydrase activity as described below.
Heterologous expression and purification of dCA I, N-ter and C-ter
Mature dCA I (Fisher et al., 1996) was expressed in the Escherichia coli Origami B strain (Novagen) and affinity purified as described previously (Premkumar et al., 2003a
,b). The cDNA sequence encoding N-ter (the recombinant N-terminal repeat of dCA I) without the leader peptide (amino acid residues 55317) was amplified from the full-length cDNA clone by PCR (Fisher et al., 1996
). The forward primer (5'-GATTGGAATTCGTGAGTGAGCCTCACG-3') contained an EcoRI site and the reverse primer (5'-TGGAAAAGCTTTTAACCCTTGTACTCGT-3') contained a stop-codon and a HindIII site. The PCR product was digested with EcoRI and HindIII and cloned into likewise-digested pET21a vector (Novagen), to create pN21a. In this construct, translation of N-ter was initiated from the T7-tag in the pET21a vector. This construct was further modified by fusion of N-ter to a His6-tag by replacing the stop codon (TAA) in pN21a by a Gly codon (GGA) using a site-directed mutagenesis procedure (QuikChange, Stratagene, La Jolla, CA) with the entire pN21a as template. The primers used for mutagenesis were 5'-CGAGTACAAGGGTGGAAAGCTTGCGGCCGC-3' and 5'-GCGGCCGCAAGCTTTCCACCCTTGTACTCG-3' and the resulting vector was designated pHtN21a. Expression of N-ter from pN21a or pHtN21a in the E.coli Origami B strain was as described previously (Premkumar et al., 2003a
). For His6-tag-based affinity purification of N-ter, a 100 ml culture of transformed E.coli was lysed in 25 mM Tris-SO4, pH 8.0, 15 mM MgSO4, 0.1% Trition X-100, 0.5 mM ZnSO4, 1 mM imidazole, 20 µg/ml DNase I and 200 mM NaCl by repeated freezing and thawing as described previously (Premkumar et al., 2003a
,b). The lysate was cleared by centrifugation and mixed with Ni-NTA resin (Qiagen), pre-equilibrated with 25 mM Tris-SO4, pH 8.0, 1 mM imidazole, 300 mM NaCl. After transfer to a column, the resin was washed with the same buffer with added 20 mM imidazole and N-ter was eluted by raising the imidazole concentration to 100 mM. N-ter containing fractions were concentrated and the buffer exchanged with 20 mM bis-Tris-Cl, pH 6.5. Further purification of N-ter was achieved by anion-exchange chromatography on HiTrap Q (Amersham-Pharmacia) by elution with a 0600 mM NaCl linear gradient. The coding sequence for C-ter [the recombinant C-terminal repeat of dCA I including residues 318589 (Fisher et al., 1996
)] was amplified by PCR using 5'-GATTGGAATTCGTGAGTGAACCCAACGA-3' and 5'-GGAAAAGCTTTTAAGCAGCAGCACCGTTATATCCG-3' as forward and reverse primers, respectively. In the forward primer, the coding sequence was 5'-extended by Val and Ser codons, to mimic the N-terminal sequence of the mature dCA I (Fisher et al., 1996
). The amplified fragment, digested with EcoRI and HindIII, was cloned into the likewise-restricted pET-28a or pET21a to form pC28a and pC21a, respectively. Expression of C-ter from pC21a in the E.coli Origami B strain or from pC28a in the E.coli BL21(DE3)pLysS strain were as described previously (Premkumar et al., 2003a
,b). C-ter was purified from E.coli lysates by DEAE-Sephadex chromatography essentially as described for the purification of p40 from D.salina extracts described above. Purification by affinity chromatography on agarose-coupled PAMBS (Sigma) was essentially as described previously (Premkumar et al., 2003b
).
CO2 hydration assay
Assays were performed essentially as described elsewhere (Booth and Beardall, 1991; Fisher et al., 1996
), with some modifications. The assays were performed in a double-walled chamber kept at 04°C by circulating ice-cold water and the reaction mixtures, in 2.75 ml, contained 6.5 mM veronal buffer, pH 8.3, 10 µM ZnSO4 and the indicated concentrations of salts or inhibitors. The reaction, started by adding 250 µl of ice-cold saturated CO2 in water, was electrochemically monitored by a temperature-compensated pH meter. Activity unit is defined by [(t0 t)/t]x10, where t is the time for the pH to change from 8.3 to 7.3 in the presence of enzyme and t0 is the corresponding time for non-catalyzed pH change (Booth and Beardall, 1991
).
Bicarbonate dehydration assay
Initial rates of bicarbonate dehydration were determined at 25°C at pH from 5.6 to 7.4 and Na bicarbonate concentrations ranging from 2 to 120 mM in an Applied Photophysics stopped-flow apparatus using the changing pH indicator method (Khalifah, 1971). The bufferindicator pairs used were MES [2-(N-morpholino)ethanesulfonic acid] and chlorophenol red for pH 5.66.2 and MOPS and p-nitrophenol for pH 6.87.4 and the reactions were monitored at 574 and 400 nm, respectively. Before adding NaCl, mixtures with different buffers and pHs were adjusted to 50 mM ionic strength with Na2SO4. Each data point used to derive kinetic constants was an average of seven independent measurements. Rates of uncatalyzed reactions under the conditions of the assay were subtracted from the rates of the enzyme-catalyzed reaction. Kinetic constants were determined by fitting the data to the MichaelisMenten equation.
Esterase assay
Steady-state initial rate measurements were carried out at 25°C in a Spectronic Genesys spectrophotometer. The rate of p-nitrophenyl acetate hydrolysis was monitored by following for 3 min the change in absorbance at 348 nm, the isosbestic point for p-nitrophenol acetate and p-nitrophenolate ion (Verpoorte et al., 1967). Mixtures with 13 mM substrate were prepared by diluting a 107 mM acetone solution of p-nitrophenyl acetate (Sigma) with water or NaCl solutions to reach the desired final concentrations of substrate and salt in 3% acetone. To 975 µl of this mixture, in a 1 ml cuvette, were added 25 µl of 1 M Tris-SO4, pH 8.2 and the reaction was immediately initiated by adding 510 µl of enzyme solution. Blank runs in the absence of enzyme provided values for non-enzymatic hydrolysis rates that were subtracted from the rates measured in the presence of enzyme.
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Results |
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CO2 hydration activity
The effect of salt on dCA I activity was first determined in assays of CO2 hydration activity in the presence of Li+, Na+, K+ and Mg2+ chlorides or the potassium salts of Br and I. A comparison of various cations at up to 1.5 M (Figure 1A) revealed little effect or even enhancement of activity at the lower range of salt concentrations. At salt concentrations >1.0 M, the activity gradually declined to levels close to those measured in the absence of salt. Quantitatively, the Li+ salt exerted the least activation and highest inhibition whereas salts of Na+, K+ and Mg2+, albeit at different concentrations, enhanced the activity by nearly 2-fold. A comparison of different potassium halides (Figure 1B) indicated an enhancement of activity at relatively low concentrations of Cl and Br but not I salts. At higher concentrations, Br was more inhibitory than Cl and I was inhibitory already at 0.1 mM, but the inhibition was not meaningfully increased up to 100 mM. These patterns of response can be understood as reflecting activation by cations such as Na+, K+ or Mg2+ superimposed on moderate inhibitory effects of anions such as Cl or Br. Thus, the interplay between stimulatory and inhibitory effects resulted in the maintenance of the enzymatic activity within relatively narrow limits over widely varied concentrations of naturally abundant salts.
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The unusual responses of dCA I to salts, as compared with hCA I, prompted us to examine the effects of several potent carbonic anhydrase inhibitors, i.e. N3, ClO4, PAMBS and acetazolamide, on the activity of both dCA I and hCA I (Figure 1C). The results indicated that dCA I resembled hCA I in sensitivity to sulfonamides and ClO4 but dCA I was distinctly more resistant to N3 than hCA I. To evaluate these data in a broader context, the inhibitory effects of salts and inhibitors on the CO2 hydration activity of dCA I and hCA I were expressed as apparent I50 values (Carlier et al., 1999) and compared with corresponding values (reported as such or converted from Ki) for human carbonic anhydrase II (hCA II) and the periplasmic carbonic anhydrase(s) (cCA) from the unicellular fresh water alga Chlamydomonas reinhardtii (Table I). The compiled data indicate that despite the large variation in inhibitor sensitivity shown by the different
-type carbonic anhydrases, dCA I stands out with its apparently low affinity for Cl and Br and its relatively low sensitivity to I and N3. On the other hand, the sensitivity of dCA I to the sulfonamides and ClO4 stays within the range exhibited by the other carbonic anhydrases included in the comparison.
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To address the possible contribution of the tandemly repeated structure to the outstanding salt tolerance of dCA I, as reflected in CO2 hydration activity, the full-length protein was compared with truncated variants including only a single complete -type carbonic anhydrase domain (cf. Figure 2).
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Enzymatic activity and salt responses were also studied with N-ter and C-ter, separately expressed in E.coli (Figures 2 and 4A). The recombinant C-ter was first purified by ion-exchange chromatography and at later times by affinity chromatography on immobilized PAMBS. As N-ter failed to bind to the carbonic anhydrase-specific affinity resin, it was fused to a His6-tag, which permitted its purification on an Ni-NTA column. The recombinant C-ter exhibited a specific activity of 450 units/mg protein, closely resembling the value determined for p40, whereas the homogeneously purified N-ter showed no detectable CO2 hydration activity (data not shown).
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Bicarbonate dehydration activity of dCA I and C-ter
Initial rates of bicarbonate dehydration by dCA I were determined in a stopped-flow spectrometer in assay mixtures containing 2120 mM Na bicarbonate as substrate. The data fitted the MichaelisMenten equation for one but not two types of substrate-binding site, suggesting that dCA I harbored two highly similar catalytic sites or that only one of the two carbonic anhydrase domains in dCA I was responsible for the observed activity.
Apparent kcat values were determined at pHs from 6.2 to 7.4 in reaction mixtures with 0 or 0.1 M NaCl adjusted with Na2SO4 to 0.05 or 0.15 M ionic strength as described in Materials and methods (Figure 5). The pH dependence curves at the two ionic strengths were characterized by a rise below pH 6.5 and two inflection points corresponding to pKas of 6.5 and 6.87.0. A marked increase in kcat at 0.15 M compared with 0.05 M ionic strength was most pronounced at acidic pHs but persisted up to neutral pH. The increase in kcat with ionic strength differentiated dCA I from enzymes such as hCA II and human carbonic anhydrase IV (hCA IV) where similar increases in ionic strength were reported to have no effect on kcat in a similar pH range (Pocker and Miao, 1987
; Baird et al., 1997
).
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For the same reasons as specified above, additional assays of dCA I and C-ter at variable pHs and salinities were conducted with a single substrate concentration (50 mM Na bicarbonate). The results (Table III; Figure 6), expressed as V0/[E]0, indicated a parallel decline in the activities of dCA I and C-ter with a rise in pH from 5.6 to 7.4. Within this pH range, C-ter displayed 58% of the activity of dCA I. Similar analyses of N-ter (data not shown) showed that it exhibited at most 2% of dCA I activity and it was not analyzed further. The activity of dCA I was practically unaffected at
0.25 M NaCl and at most
40% reduced at 0.5 M NaCl. In comparison, the responses of C-ter were markedly more sensitive to salt and pH variations. The bar diagram in Figure 6 shows the effects of salt at each pH normalized to the corresponding activity in the absence of salt. The moderate effects of pH changes on the salt responses of dCA I contrast with the large fluctuations exhibited by C-ter, including inhibition at pH 5.6, salt concentration-dependent inhibitory or stimulatory responses at pH 6.2 and 6.8 and stimulatory effects at pH 7.4.
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The esterase activity of -type carbonic anhydrases shares several basic characteristics with reversible CO2 hydration activity, although some disparities have also been documented (Pocker and Stone, 1967
; Verpoorte et al., 1967
; Baird et al., 1997
; Elleby et al., 1999
). Preliminary experiments demonstrated that dCA I possessed esterase activity and indicated that, similarly to hCA I, it preferred p-nitrophenyl acetate over o-nitrophenyl acetate as a substrate (data not shown). Assays under the same conditions for N-ter and C-ter at up to
3 µM protein did not reveal any esterase activity.
The pH dependence curve for the catalysis of p-nitrophenyl acetate hydrolysis by dCA I corresponded to a pKa of 7.3 (data not shown), similar to the value reported for hCA I, suggesting that the same ionizable group(s) may be involved in catalysis by the two enzymes (Pocker and Stone, 1967).
As pointed out in earlier studies (Verpoorte et al., 1967), the low water solubility of the substrate made it difficult to reach concentrations high enough to approach Km values. However, kinetic data with 13 mM substrate at 02.0 M NaCl, obtained from slopes of LineweaverBurk plots, permitted the determination of kenz values for dCA I under these conditions (Table IV). The results indicated that kenz for dCA I increased with salt up to
2-fold at 2.0 M NaCl. In comparison, the kenz for hCA I was
80% reduced at 0.1 M NaCl, in line with the reported inhibitory effects of anions on esterase activity of several
-type carbonic anhydrases (Pocker and Stone, 1967
; Thorslund and Lindskog, 1967
; Maren and Couto, 1979
).
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Discussion |
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As manifested in CO2 hydration assays, dCA I differed drastically from hCA I and other mesophilic counterparts in remaining active in the presence of molar concentrations of Cl and Br and in showing a lowered sensitivity to I and N3. However, the fact that dCA I was inhibited similarly to hCA I by ClO4 and sulfonamides suggests a similarity in the affinity of these inhibitors to the two enzymes. The differential responses of dCA I to the two classes of inhibitor may be related to their different modes of interaction with the protein as suggested by structure determinations of proteininhibitor complexes. Thus, Br or N3 complexed with hCA II and I complexed with hCA I were coordinated to the Zn in a distorted tetrahedral geometry (Jonsson et al., 1993; Nair and Christianson, 1993
; Liljas et al., 1994
), whereas sulfonamides coordinated to the Zn in a regular tetrahedral configuration stabilized by H-bonding to the O
1 of Thr199 (Liljas et al., 1994
). Still, these differences cannot account for the apparently exceptional low affinity of the enzyme to halide and azide anions.
Aside from its apparent low affinity for anions, dCA I is up to 2-fold stimulated in CO2 hydration activity by cations such as Na+, K+ or Mg2+. Although activation of various -type carbonic anhydrases by a host of organic compounds, including amines and amino acids, has been widely documented (Supuran and Scozzafava, 2000
), cation activation as observed for dCA I appears to have no parallel in hCA I and other, homologous enzymes. Other possible manifestations of cation activation of dCA I include the rise in kenz for esterase activity with increasing NaCl concentrations [for effects of salt on esterase activity of other carbonic anhydrases, see elsewhere (Pocker and Stone, 1967
; Thorslund and Lindskog, 1967
; Steiner and Lindskog, 1972
; Maren and Couto, 1979
)] and the increase in turnover number for bicarbonate dehydration with ionic strength.
The pH dependence curve of kcat is consistent with pKas of 6.5 and 6.87, originating in still unidentified ionizable groups. In other -type carbonic anhydrases, pKas were attributed to Zn-bound water and the proton-shuttling residue His64, present in hCA II, hCA IV and murine carbonic anhydrase VII (mCA VII) (Earnhardt et al., 1998
). Another proposal, perhaps relevant to dCA I, ascribes one of two pKas observed for the kcat for CO2 hydration activity of mCA VII to perturbed ionization of Zn-bound water by electrostatic interactions with nearby groups (Earnhardt et al., 1998
). However, according to sequence alignments, dCA I does not include His64 or other residues so far implicated in proton shuttling in other enzymes, e.g. His200 in hCA I and Tyr131 in human carbonic anhydrase V (hCA V) (Baird et al., 1997
; Qian et al., 1999
).
The degree and mode of anion inhibition of various -type carbonic anhydrases have been shown to be pH dependent (Pocker and Stone, 1967
; Baird et al., 1997
). This dependence has been linked to the ionization of the Zn-bound water and taken to reflect a higher affinity of anions to water-bound compared with hydroxyl-bound Zn (Baird et al., 1997
). Thus, the Ki for sulfate inhibition of bicarbonate dehydration activity of hCA IV gradually rose to reach a 5-fold increase as the pH was shifted from 5.5 to 7.0 (Baird et al., 1997
). In comparison, the bicarbonate dehydration activity of dCA I showed only moderate or no response to salt at pH 5.67.4 (cf. Table III). This behavior suggests that ionizable groups unique for dCA I may modulate long-range interactions so as to generate a buffering capacity that maintains the salt responses of dCA I within relatively narrow limits over a broad pH range.
The unique properties of dCA I can be partly attributed to its two-domain structure. Multiple sequence alignments indicated the conservation in both N- and C-terminal domains of the Zn-coordinating residues His94, His96 and His119, the hydrophobic residues in the substrate-association pocket Val121, Leu141, Val143, Leu198, Val207 and Trp209 and the residues involved in the active-site hydrogen-bonded network involving Glu106 and Thr199 (cf. Figure 2). The kinetic analysis was compatible with the notion that dCA I harbored only a single type of substrate-binding site. Alternatively, the results may suggest that despite their sequence similarity, only one of the two domains is catalytically active. That this site might be localized to the C-terminal domain is supported by analyses of truncated derivatives of dCA I showing that C-ter, but not N-ter, bound to the PAMBS affinity column and, similarly to p40, was enzymatically active. Still, the reduced activity of the single-domain derivatives relative to dCA I points to the large contribution of inter-domain interactions to the enzymatic efficiency.
The salt responses of CO2 hydration activity catalyzed by C-ter and p40 largely recapitulated the responses of dCA I, albeit to a lower extent, indicating that essential elements required for salt tolerance are localized, although not fully expressed, in the C-terminal domain. The apparent enhancement of substrate affinity (lowering of Km for bicarbonate) by salt observed in bicarbonate dehydration assays of C-ter, but not of dCA I, most likely reflected the stabilization by salt of C-ter, perhaps mimicking interactions of the C-terminal with the N-terminal domain within dCA I. Similar interactions may also be invoked to explain the differential effects of pH on the salt responses of C-ter and dCA I as expressed in bicarbonate dehydration activity. Overall, these results indicate that inter-domain interactions play a decisive role in the salt responses of dCA I.
Attempts to elucidate these interactions further via the reconstitution of a native-like enzyme by mixing N-ter and C-ter have so far been unsuccessful (data not shown). However, stable inter-domain interactions were indicated by observations with proteolytically cleaved dCA I. The p20 and p40 sub-fragments of dCA I (Figure 2) partly co-fractionated on both affinity and ion-exchange columns (cf. Figure 3 and unpublished data). Furthermore, purified recombinant dCA I, with six extra amino acids inserted between the N- and C-terminal repeats, was spontaneously cleaved into sub-fragments similar in size to p20 and p40 and exhibited the same activity and salt tolerance as the uncleaved dCA I (data not shown). These results strongly support the existence of stable interactions between the N- and C-terminal domains within the structure of dCA I.
Another structural distinction of dCA I, potentially linked to salt tolerance, lies in its biased amino acid composition (Fisher et al., 1996), reminiscent of proteins from extreme halophilic archaea (Mevarech et al., 2000
; Fukuchi et al., 2003
) but atypical for most other
-type carbonic anhydrases (Gokhman et al., 1999
). Like halophilic proteins, the amino acid composition of dCA I is characterized by a decreased proportion of basic amino acid residues, particularly Lys, while the contents of Asp and Glu remain largely unchanged.
The most outstanding characteristic of halophilic enzymes is their obligatory requirement for multimolar concentrations of KCl or NaCl for proper folding and conformational stability (Lanyi, 1974; Eisenberg et al., 1992
; Madern et al., 2000
; Mevarech et al., 2000
). The effects of various salts on the conformational stability of the best-characterized halophilic enzyme, malate dehydrogenase from Haloarcula marismortui, was interpreted as reflecting the superposition of independent contributions of cations and anions, partly in accordance with the Hofmeister series, which classifies ionprotein interactions according to their effect on solubility and stability (Ebel et al., 1999b
). Proposed mechanisms to account for the obligatory salt requirement of halophilic proteins include, among others, the formation of hydrated cation networks on clusters of negative charges and ion binding to specific sites on the folded structure (Dym et al., 1994
; Frolow et al., 1996
; Madern et al., 2000
).
Electrostatic potential calculations, based on homology modeling of the C-terminal repeat of dCA I, indicated a highly negative surface potential (data not shown) contrasting with other -type carbonic anhydrases. Similarly to halophilic proteins, a hydrated cation network may promote the global stability of the protein at high salinities. However, dCA I does not exhibit an obligatory salt requirement for proper folding and activity. In further support of the latter conclusion, we observed that dCA I, unfolded in 8.0 M urea, could be refolded to a fully active form in the absence of salt. Circular dichroism spectra of dCA I taken in the presence of 03.0 M NaCl did not indicate any conformational changes in dCA I throughout this salinity range (unpublished data).
In conclusion, the present analyses provide clear evidence that dCA I is a uniquely salt-tolerant protein, i.e. retains an active conformation over a broad range of salinities and, as a Zn metalloenzyme, largely avoids the specific inhibitory effects of anions. A detailed elucidation of the structural features underlying the outstanding salt responses of dCA I as well as of dCA II, currently under way, will contribute to the fundamental understanding of halotolerance and its implied applications.
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Acknowledgements |
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Received December 30, 2003; accepted January 27, 2004 Edited by Dan Tawfik