Nephrology Department, Urology and Nephrology Center, Mansoura University, Mansoura, Egypt
Correspondence and offprint requests to: Professor Dr Mohamed A. Sobh, MD, FACP, Nephrology Department, Urology and Nephrology Center, Mansoura University, Mansoura, Egypt. Email: sobh10{at}yahoo.com
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Abstract |
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Methods. The subjects of this work included 70 live-donor stable kidney transplant recipients receiving tacrolimus. Their age ranged from 16 to 45 years. Among them, 54 were males and 16 were females. All of them were 6 months or more post-transplantation. Patients were randomly divided into two equal groups. Group I patients initially received ketoconazole 100 mg/day in addition to their usual treatment, while group II patients were considered a control. Patients were followed-up for 6 months.
Results. Concomitant ketoconazoletacrolimus resulted in marked reduction of tacrolimus dose (by 58.7%) and cost (by 56.9%). It also resulted in significant improvement in graft function and fungal skin infection, in addition to a decrease of gastrointestinal episodes and hospitalization.
Conclusion. We conclude that ketoconazoletacrolimus combination in kidney transplant recipients is safe, has outstanding impact on treatment costs and improves patient and graft outcome.
Keywords: ketoconazole; kidney transplantation; tacrolimus
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Introduction |
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Both tacrolimus and cyclosporine are metabolized in the liver through the hepatic cytochrome P450 system. In man, cytochrome P450 is also present in the upper gastrointestinal tract [4].
Drugs that inhibit the cytochrome P450 system will increase tacrolimus and cyclosporine levels. This may be of therapeutic benefit. For example, diltiazem makes a logical cyclosporine and tacrolimus-sparing agent because of the potential financial savings and therapeutic benefits [5].
Ketoconazole, an imidazole antifungal drug, was reported to interact with tacrolimus and cyclosporine through inhibition of cytochrome P450 microsomal enzymes in the liver, leading to inhibition of tacrolimus and cyclosporine metabolism and subsequently increasing their blood levels [4,6]. Additionally, ketoconazole increases tacrolimus and cyclosporine bioavailability through inhibition of their gut metabolism [7,8].
Long-term safety and financial benefits of the ketoconazolecyclosporine combination were recently reported by Sobh et al. [9]. They stated that this combination resulted in a marked reduction of cyclosporine dose with a cost saving of 70% and excellent long-term safety.
However, similar clinical trials concerning safety and financial benefits of the tacrolimusketoconazole combination after kidney transplantation are scarce. Thus, our work was conducted in order to study this interesting subject.
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Subjects and methods |
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Study design
After baseline evaluation, patients were randomly divided into two equal groups. Group I (ketoconazole group) patients received ketoconazole 100 mg/day in addition to their usual treatment while group II served as a control. In group I patients, information about ketoconazole was provided prior to its prescription and concomitant to its first administration, tacrolimus dose was reduced by 50%.
Patients were prospectively followed up for 6 months in the outpatient clinic once weekly for 1 month, every other week for 2 months and every month thereafter.
At each visit, clinical, laboratory and radiological assessment was performed. Thorough clinical evaluation was done with emphasis on blood pressure, fungal skin infection and drug tolerance. Hypertension was generally defined as blood pressure >140/90 mmHg or maintenance on anti-hypertensive medications. Laboratory evaluation included monitoring of graft function by serum creatinine, creatinine clearance, urine analysis and 24 h urinary proteins, liver function tests, serum electrolytes, cholesterol, uric acid, prothrombin concentration and tacrolimus whole blood trough level (MEIA II; Abbott Imx analyser). In patients who experienced unexplained graft dysfunction, graft biopsy was performed. The target tacrolimus whole blood trough level was 37 ng/ml. The tacrolimus dose was adjusted according to the drug level. The ketoconazole dose in the ketoconazole group was reduced to 50 mg/day in patients who experienced high levels of tacrolimus despite reduction of the tacrolimus dose to 1 mg/12 h.
Statistical analysis
Continuous values were expressed as means±standard deviation and compared by t-test (paired for comparison within the same group and unpaired for comparison between the two groups). Non-homogeneous data were expressed as median/range and compared by a MannWhitney test. Numeric data were compared using a chi-square test between the two groups.
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Results |
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Tacrolimus trough levels were comparable in both groups throughout the study (Figure 1). During the first month of the study, ketoconazole-treated patients were monitored weekly for their tacrolimus trough level. All levels were within the therapeutic window, except for three patients whose tacrolimus level at the first week ranged from 10 to 11 ng/ml.
At the end of the study, percentage reduction of tacrolimus cost in the ketoconazole group (after addition of ketoconazole cost) was 56.9%, while it was only 8.4% in the control group. The difference between the two groups was highly significant (P<0.001). Calculated from the cost at the end of the study, the yearly tacrolimus cost saving per patient in the ketoconazole group was $3173.66 compared with $370.29 in the control group.
Total days of hospitalization were significantly lower in the ketoconazole group throughout the study period (16 vs 38 days; P = 0.01). The commonest cause of hospitalization in both groups during the study was gastrointestinal episodes (vomiting and/or diarrhoea) followed by graft impairment episodes. Both aetiologies were significantly lower in the ketoconazole group (P = 0.02 and 0.005, respectively).
Initial serum creatinine at the start of the study was insignificantly higher in the ketoconazole group (mean = 1.74 mg/dl) compared with the control group (mean = 1.52 mg/dl). Subsequently, serum creatinine values remained comparable in both groups throughout the study (Figure 2). However, within the ketoconazole group, serum creatinine values on all follow-up occasions became lower than their initial serum creatinine value. This difference was highly significant (P<0.001) at the first, fourth and sixth months, significant (P = 0.01) at the second month, but did not rank to significance at the third and fifth months (P = 0.36 and 0.46, respectively). In contrast, serial serum creatinine values in the control group were higher than initial serum creatinine and this difference was highly significant (P<0.001) at the first month and significant thereafter (P<0.05) except for the fifth month (P = 0.08).
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Fasting blood sugar was comparable in both groups throughout the whole follow-up period. The number of patients with post-transplant diabetes mellitus remained the same throughout the study. Additionally, these patients remained comparable regarding type and dose of anti-diabetic agents.
Serum bilirubin, ALT and AST were comparable in both groups throughout the study. Serum albumin levels were comparable in both groups at the start and during the first 4 months of the study. However, their levels (g/dl) became significantly higher in the ketoconazole group compared with the control group at the fifth month (4.06±0.33 vs 3.83±0.34, P = 0.01) and at the sixth month (4.02±0.33 vs 3.76±0.36, P = 0.002).
Twenty-four hour urinary protein, serum cholesterol, haemoglobin, haematocrit and prothrombin concentration values were comparable in both groups throughout the study.
The incidence of hypertension in the ketoconazole group decreased from 65.7% before to 57.14% at the end of the study and remained constant (60%) in the control group. However, this apparent difference between the two groups did not rank to statistical significance.
At the start of the study, the prevalence of fungal skin infection in the form of tinea pedis, tinea versicolor and tinea circinata was significantly higher in the ketoconazole group (nine patients; 25.7%) than in the control group (two patients; 5.7%) (P = 0.02). Fungal skin infection in the control group patients was treated with topical anti-fungal creams and terbinafine (systemic anti-fungal agent that has no interaction with tacrolimus). At the third month of follow-up, the number of patients with fungal skin infection in the ketoconazole group dramatically decreased to only two patients (2.8%), similar to that in the control group. At the end of the study, there was complete recovery in the ketoconazole group, while one case in the control group was still resistant to therapy.
In group I, the dose of ketoconazole was reduced to 50 mg/day in four patients; one at the second month of the study and three at the last follow-up.
In general, ketoconazole-treated patients showed good tolerance to the drug. None of ketoconazole's side effects was noticed and none of the patients stopped the drug at any time during the study.
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Discussion |
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The financial benefit of tacrolimus dose reduction in the ketoconazole-treated group was outstanding. The yearly tacrolimus cost saving for the whole ketoconazole group (35 patients) was $11 1077.98. Expanding the kidney transplantation programme in our developing country is hindered by financial problems, especially due to costly newer immunosuppressive medications such as tacrolimus. Concomitant use of ketoconazole in such patients will result in a marked reduction of tacrolimus dose and cost by 60%. Furthermore, it may help the use of this medication in non-transplant indications such as in patients with idiopathic nephrotic syndrome [10] in whom cost stands as a major barrier.
Marked reduction in frequency of gastrointestinal and graft impairment episodes necessitating hospitalization among the ketoconazole group, with possible added cost saving and better patient quality of life, may be due to a marked reduction of tacrolimus dose. The tendency of transplant patients towards stability after the early post-transplant period is a possible contributing factor as there was a shorter, despite statistically insignificant, mean post-transplant period in the ketoconazole group. Additionally, other immunosuppressive agents may have a role in increased incidence of gastrointestinal episodes. More than half of patients in both groups were maintained on MMF. As diarrhoea is a possible, however not a very typical, adverse event in patients treated with tacrolimus, many of the gastrointestinal problems might be due to MMF treatment.
The higher incidence of post-transplant diabetes mellitus among the ketoconazole group patients, though statistically insignificant, may be due to a significantly higher initial dose of tacrolimus in that group. However, it is known that the trough level rather than the dose of tacrolimus is linked to higher incidence of diabetes mellitus [3], which may explain our subsequent observation that, despite a marked reduction of tacrolimus dose in the ketoconazole group throughout the study, the prevalence of diabetes mellitus remained the same. On the other hand, another finding in the ketoconazole group was the decreased prevalence of hypertension among its patients throughout the study; a finding that may be explained by the significant reduction in the serum creatinine and/or tacrolimus dose at the end of the study.
The interesting observation of improved graft function in the ketoconazole group may be explained by a marked reduction of tacrolimus dose in this group. Tacrolimus nephrotoxicity may be merely functional with deterioration of graft function, despite the normal range of tacrolimus levels. Improvement in graft function may occur with reduction of tacrolimus dose [11]. On the other hand, lowering of graft function in the control group may be attributed to more than one factor, including drug nephrotoxicity or more gastrointestinal and acute rejection episodes.
Although statistically insignificant, the lower incidence of acute rejection episodes in the ketoconazole group may be explained by its inhibitory effect on glucocorticoid metabolism [12]. It may also be explained by the possible effect of ketoconazole on phagocytic, lymphocytic and natural-killer cell response [13]. Long-term follow-up may support this observation.
Although ketoconazole is known to be hepatotoxic [14], this side effect was not observed in any of our ketoconazole group patients. Ketoconazole-associated hepatotoxicity may be idiosyncratic in nature [15]. This observation may also be attributed to the small dose of ketoconazole used (50100 mg/day) and/or the marked reduction in tacrolimus dose which has a potential hepatotoxicity [16].
Our study documents the efficacy of ketoconazole in preventing and treating fungal skin infection in kidney transplant recipients. These results provide an added benefit for ketoconazole use in kidney transplant recipients in whom fungal skin infections are not uncommon [17], especially in communities with lowered socio-economic and hygiene standards.
In general, ketoconazole-treated patients showed good tolerance to the drug. None of ketoconazole's side effects was noticed and none of the patients stopped the drug at any time during the study. Similarly, Buttman et al. [18] did not report side effects of ketoconazole when concomitantly used with cyclosporine in heart transplant recipients. Conversely, First et al. [19] reported discontinuation of ketoconazole therapy in four out of 43 kidney transplant recipients when used concomitantly with cyclosporine, mainly due to gastrointestinal intolerance and sexual dysfunction. This difference in ketoconazole tolerance may be due to different dose regimens, number of patients and/or genetic factors. All patients in our study were compliant to treatment with ketoconazole. It seems that our previous experience with ketoconazole in the field of kidney transplantation [9] allowed for better physician experience and patient compliance.
In conclusion, concomitant ketoconazoletacrolimus administration in kidney transplant recipients is safe and results in outstanding dose reduction. Moreover, it results in significant improvement of graft function, fungal skin infection as well as significant reduction in the frequency of gastrointestinal episodes and patient hospitalization. The great financial benefit of this combination will allow wider use of tacrolimus in transplantation as well as in other immune-mediated diseases. However, long-term follow-up is recommended to firmly document the safety and the possible beneficial effects of this combination.
Conflict of interest statement. None declared.
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References |
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