The Small Heterodimer Partner Interacts with the Pregnane X Receptor and Represses Its Transcriptional Activity
Jean Claude Ourlin,
Fredéric Lasserre,
Thierry Pineau,
Jean Michel Fabre,
Antonio Sa-Cunha,
Patrick Maurel,
Marie-José Vilarem and
Jean Marc Pascussi
Institut National de la Santé et de la Recherche Médicale, Unité 128, Institut Fédératif de Recherche 24 (J.C.O., P.M., M.-J.V., J.M.P.), Centre National de la Recherche Scientifique, 34293 Montpellier Cedex 05, France; Laboratoire de Pharmacologie et Toxicologie (F.L., T.P.), Institut National de la Recherche Agronomique, 31931 Toulouse, Cedex 9, France; Service de Chirurgie (J.M.F.), Hôpital Saint Eloi, 34295 Montpellier Cedex 05, France; and Service de Chirurgie Digestive (A.S.-C.), Hôpital Haut Levèque, 33600 Pessac, France
Address all correspondence and requests for reprints to: Jean Marc Pascussi, Institut National de la Santé et de la Recherche Médicale, Unité 128, Institut Fédératif de Recherche 24, Centre National de la Recherche Scientifique, 1919 Route de Mend, 34293 Montpellier Cedex 05, France. E-mail: pascussi{at}montp.inserm.fr.
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ABSTRACT
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SHP (small heterodimer partner, NR1I0) is an atypical orphan member of the nuclear receptor subfamily in that it lacks a DNA-binding domain. It is mostly expressed in the liver, where it binds to and inhibits the function of nuclear receptors. SHP is up-regulated by primary bile acids, through the activation of their receptor farnesoid X receptor, leading to the repression of cholesterol 7
-hydroxylase (CYP7
) expression, the rate-limiting enzyme in bile acid production from cholesterol. PXR (pregnane X receptor, NR1I2) is a broad-specificity sensor that recognizes a wide variety of synthetic drugs as well as endogenous compounds such as bile acid precursors. Upon activation, PXR induces CYP3A and inhibits CYP7
, suggesting that PXR can act on both bile acid synthesis and elimination. Indeed, CYP7
and CYP3A are involved in biochemical pathways leading to cholesterol conversion into primary bile acids, whereas CYP3A is also involved in the detoxification of toxic secondary bile acid derivatives. Here, we show that PXR is a target for SHP. Using pull-down assays, we show that SHP interacts with both murine and human PXR in a ligand-dependent manner. From transient transfection assays, SHP is shown to be a potent repressor of PXR transactivation. Furthermore, we report that chenodeoxycholic acid and cholic acid, two farnesoid X receptor ligands, induce up-regulation of SHP and provoke a repression of PXR-mediated CYP3A induction in human hepatocytes as well as in vivo in mice. These results reveal an elaborate regulatory cascade, tightly controlled by SHP, for both the maintenance of bile acid production and detoxification in the liver.
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INTRODUCTION
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NUCLEAR RECEPTORS CONSTITUTE a superfamily of ligand-modulated transcription factors that mediate cellular response to small lipophilic endogenous and exogenous ligands. They consist of a variable N-terminal domain, often exhibiting a constitutive transcription activation function (AF-1), a highly conserved zinc finger type DNA-binding domain (DBD), a variable linker region, and a multifunctional C-terminal domain that is responsible for ligand binding (LBD), dimerization, and ligand-induced transcriptional AF-2 (1). Most nuclear receptors heterodimerize with the retinoid X receptor (RXR).
SHP (short heterodimer partner) is an orphan nuclear receptor that lacks a conventional DBD and is mostly expressed in the liver (2). In spite of the lack of a DBD or of a conventional nuclear localization signal, it appears to be located in the nucleus of mammalian transfected cells (3). A series of reports have shown that SHP is able to interact with and inhibit the transcriptional activity of several members of the nuclear receptor superfamily including the constitutive androstane receptor (CAR), thyroid hormone receptor (TR), retinoid X receptor (RXR), retinoid acid receptor (RAR), androgen receptor, estrogen receptor (ER), the liver receptor homolog-1 (LRH-1), liver X receptor
and ß, and hepatocyte nuclear factor-4 (HNF4) (3, 4, 5, 6, 7, 8, 9, 10, 11). SHP has been shown to inhibit RAR:RXR heterodimer DNA binding and to inhibit the transcriptional activity of RAR, mCAR, and ER. Moreover, as SHP contains a strong transcriptional repression domain in its C terminus (3), it has been suspected to act as a direct transcriptional repressor by recruiting conventional corepressors such as nuclear receptor corepressor, as reported with DAX-1 (DSS-AHC critical region on X chromosome, gene 1) (12). However, further analysis failed to demonstrate a direct interaction between SHP and nuclear receptor corepressor (3). Alternatively, SHP has recently been proposed to represent a new category of nuclear receptor coregulator, interfering directly with AF-1 and AF-2 coactivator factors such as coactivator four-and-a-half-LIM-only protein FHL2, steroid receptor coactivator-1 (SRC-1), transcription intermediary factor 2, or receptor interacting protein with a molecular mass of 140 kDa, and two possibilities have been considered in this respect: 1) SHP and AF-1/2 coactivators may compete for a common site; or 2) binding of SHP to the receptor may induce conformational changes leading to the dissociation of AF-1/2 coactivators from the receptors (3, 5, 13). Taken together, these studies suggest that SHP inhibits the transcriptional activity of nuclear receptors by several mechanisms.
Recently, SHP has been shown to be involved in the control of bile acid biosynthesis, the first and rate-limiting step of which is catalyzed by cytochrome P450 7
(CYP7
), a liver-specific enzyme (14). Two different laboratories (4, 9) have reported that upon activation by primary bile acids such as chenodeoxycholic acid (CDCA), farnesoid X receptor (FXR) induces the expression of SHP, which then binds to and inhibits LRH-1, an orphan receptor that regulates CYP7
expression (15).
CYP3A enzymes are known to be involved in the oxidative metabolism of many xenobiotics as well as of endogenous compounds such as steroids (16). More recently, these enzymes have been shown to play a role in bile acid catabolism and biosynthesis (17, 18). Fuster and Wikvall (18) demonstrated that microsomal 25-hydroxylation of 5ß-cholestane-3
,7
,12
-triol, a known bile acid precursor, is catalyzed mainly by CYP3A4. In addition, Honda et al. (19) demonstrated that microsomal 25- and 26-hydroxylation of the cholesterol side chain are catalyzed by cyp3a11 in CYP27-/- mice, and they provided strong arguments in favor of the implication of human CYP3A4 in a similar function. Notably, using human liver microsomes, they reported that the rates of 5ß-cholestane-3
,7
,12
-triol 25- and 26-hydroxylation and 5ß-cholestane-3
,7
,12
, 25-tetrol 23R-,24R-, 24S- and 27-hydroxylation are strongly correlated with the rate of 6ß-hydroxylation of testosterone, a marker of CYP3A4. Finally, Cheng et al. (20) reported in 1977 that phenobarbital, a well-known CYP3A inducer, produces an increase in the rate of hydroxylation of 5ß-cholestane-3
,7
,12
-triol and 5ß-cholestane-3
,7
,12
,25-tetrol in rats. These metabolite products can be either excreted from the body in the bile or urine or converted to cholic acid (CA).
PXR (pregnane X receptor) was first reported to control CYP3A gene induction upon activation by xenobiotic inducers such as phenobarbital and rifampicin (RIF) (21). More recently, it has been shown that PXR is a functional receptor of the bile acid precursors (5ß-cholestan-3
-7
-12
-triol; and 5ß-cholestan-3
-7
-12
-25-tetrol) (22, 23), which represent CYP3A substrate and product, respectively. PXR is also activated by secondary bile acid derivatives such as lithocholic acid (24). Upon activation, PXR controls elimination of these compounds by inducing CYP3A4 (21) and Oatp2 (24, 25) expression. However, the concentration of lithocholic acid required to activate PXR appears to be higher than those that occurs in vivo.
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A Series of Recent Reports Suggest that CYP3A Expression Is Negatively Regulated by FXR Ligands and/or FXR
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1) Taurochenodeoxycholic acid, a FXR ligand (26), reduced CYP3A-associated monooxygenase activities in vivo in rats (27). 2) Cyp3a11 up-regulation observed in CYP27 -/- mice is reversed if mice are fed with diets containing CA or CDCA (22). 3) Handschin et al. (28) reported that cotreatments with CA or CDCA and phenobarbital or clotrimazole (activators of CAR and PXR, respectively) reduces CYP3A induction in a chicken hepatoma cell line LMH. 4) Finally, Schuetz et al. (29) observed a strong increase in the expression of cyp3a11 and cyp2b10 in FXR -/- mice with respect to wild-type animals. However, the molecular mechanisms involved remain largely unknown. As FXR has been shown not to bind to PXR- or CAR-responsive elements (28), we suspected that these negative effects on CYP3A expression could be mediated through FXR-induced SHP up-regulation. Experiments reported here, using both in vitro and in vivo experiments, show that the transcriptional activity of the ligand-activated form of PXR is repressed by SHP.
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RESULTS
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PXR Interacts with SHP in a Ligand-Dependent Manner
Interaction between SHP and PXR was investigated biochemically in vitro, using the glutathione-S-transferase (GST) pull-down assay. As indicated in Fig. 1A
, 35S-methionine-labeled murine PXR.1 interacted with the GST-murine SHP fusion protein (mSHP) in the presence of pregnenolone 16-carbonitrile (PCN) in a dose-dependent manner (lanes 5 and 6), whereas a very faint interaction was observed in the absence of PCN (lane 4). No interaction was observed with the control GST protein (lane 3). As expected, a ligand-independent interaction was observed between GST-mSHP and mCAR (lane 2), but no interaction was observed with radiolabeled luciferase protein (lane 1). A similar interaction was observed with an opposite approach between the GST-human (h) PXR fusion protein and 35S-methionine-labeled mSHP in the presence of RIF (Fig. 1B
, lanes 2 and 3). As expected, luciferase protein failed to interact with the GST-hPXR fusion protein (lane 5), whereas no interaction was observed with the control GST protein (lane 4). Overall, these in vitro assays demonstrate that SHP interacts with PXR in a ligand-enhanced manner and suggest that PXR could be a target for SHP-mediated repression.

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Fig. 1. In Vitro Interaction between Ligand-Activated PXR and SHP in GST-Fusion Protein Based Assays
A, In vitro interaction between GST-mSHP fusion protein and mPXR.1. Murine PXR.1, CAR, or luciferase proteins were labeled with 35S-methionine by in vitro translation. GST or GST-murine SHP fusion protein were expressed in E. coli BL21 strain and tested for interaction with mPXR.1 in the absence and presence of increasing amount of PCN. B, In vitro interaction between GST-human PXR fusion protein and SHP. Murine SHP or luciferase proteins were labeled with 35S-methionine by in vitro translation. GST or GST-human PXR fusion protein were expressed in E. coli BL21 strain and tested for interaction with SHP in the absence and presence of RIF. About 20% of mSHP was used in each lane are shown as INPUT.
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SHP Inhibits the Transcriptional Activity of PXR
SHP has previously been shown to act as a negative regulator of several receptor signaling pathways in mammalian cells (2). The effect of SHP on the transcriptional activity of mouse (m) PXR and hPXR was investigated by transient cotransfections using a reporter plasmid containing only three copies of the PXR response element of the mouse CYP3A promoter in front of the minimal thymidine kinase promoter (30), i.e. (CYP3A1 DR3)3-tkCAT, or the previously described reporter construct containing the proximal CYP3A4 promoter (-1100/+43) linked to the distal xenobiotic-responsive element module (XREM) of the human CYP3A4 gene (31, 32) i.e. p(CYP3A4 XREM[-7800/+7200]/-1100/+43)-pGL3-LUC. These reporter plasmids were cotransfected with mPXR or hPXR expression plasmids and increasing amounts of mSHP or hSHP expression plasmids, respectively. The results obtained in CV1 cells are shown in Fig. 2
. In these experiments, PCN and RIF were used as specific ligands of mPXR and hPXR, respectively. It clearly appears that cotransfection of increasing amounts of mSHP or hSHP expression plasmids resulted in a dose-dependent decrease in the ligand-induced transactivation of both reporter constructs by mPXR (Fig. 2A
) or hPXR (Fig. 2B
), with only approximately 20% of residual activity in the presence of the higher amount of SHP expression plasmids. This level of inhibition is close to that previously reported with mCAR and RXR, and stronger than that observed with RAR (2). Similar results were obtained in HepG2 cells (data not shown) so that SHP is able to interact with hPXR in two different cell lines. Consistent with the relatively weak ligand-independent interaction of SHP with PXR observed in vitro (Fig. 1
), SHP coexpression resulted in a moderate decreased of PXR transcriptional activity in the absence of ligand (2040% of inhibition). This inhibition may result from different mechanisms including: 1) SHP vs. RXR competitive binding to PXR leading to impairment of PXR:RXR heterodimer binding to DNA as suggested for RAR:RXR (10); or 2) SHP-PXR interaction involving the repression domain and/or the LBD/AF-2 domain of PXR as reported for the ER (3).

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Fig. 2. SHP Inhibits PXR-Dependent Transactivation in Transient Transfection Assays
A, CV1 cells were cotransfected with 50 ng of mPXR.1 expression plasmid and increasing amounts of mSHP expression plasmid in combination with 250 ng of the (CYP3A DR3)3 tk-CAT reporter and pSV-ß-galactosidase (25 ng) as internal control. Cells were then incubated 24 h with solvent (NT) or 5 µM PCN, and assayed for CAT and ß-galactosidase activities. B, CV1 cells were cotransfected with 100 ng of pSG5- ATG-hPXR expression plasmid and increasing amount of hSHP expression plasmid in combination with 250 ng of p(CYP3A4/XREM/-1100/+43)-tk-LUC reporter and pSV-ß-galactosidase (25 ng) as internal control, as indicated. Cells were then incubated for 24 h with solvent (NT) or 5 µM RIF, and assayed for luciferase and ß-galactosidase activities. All values represent the mean of duplicate samples, and are derived from a representative experiment among three independent ones.
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SHP Inhibits PXR:RXR Heterodimer DNA Binding
Although its closest relative receptor, DAX-1, is able to bind to the retinoid acid response element of the RAR element ß2 promoter (33), SHP is not (3, 10). Using gel mobility shift assays and in vitro synthesized proteins, we confirmed that SHP alone or in the presence of either RXR or PXR does not bind to the CYP3A4 ER6 oligonucleotide (not shown), whereas, as expected, the PXR:RXR
heterodimer does (Fig. 3
, lane 4). We then verified that this complex is displaced by an excess of ER6 unlabeled oligonucleotide (lane 5). Interestingly, bacteria-expressed GST-SHP inhibited the binding of PXR:RXR heterodimer to the ER6 in a dose-dependent manner (lanes 89), whereas comparable amounts of GST did not (lanes 67). It is tempting to propose from these results that the inhibitory effect of SHP on PXR transcriptional activity results from the formation of a SHP:PXR heterodimer that is unable to bind the PXR-responsive element. However, as SHP is able to interact with RXR in the absence of ligand, this inhibitory effect could merely result from a depletion of RXR through the formation of SHP:RXR heterodimers.

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Fig. 3. SHP Inhibits PXR:RXR Heterodimer Binding to CYP3A4 ER6 Oligonucleotide
EMSAs were performed with a 32P-labeled oligonucleotide containing the ER6 PXR binding site from the human CYP3A4 promoter and in vitro synthetized ATG-hPXR and RXR in combination with increasing amounts of purified GST-mSHP or GST. The positions of the shifted PXR: RXR: ER6 complex (PXR:RXR), nonspecific band (N.S.) and free probes are indicated.
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SHP Inhibits the Transcriptional Activity of a Positive Regulator of Galactose Inducible Genes in Yeast (GAL4)-hPXR Ligand Binding Domain Fusion Protein
In an attempt to determine whether the inhibitory effect of SHP involves the LBD of PXR, we used a fusion protein consisting of the GAL4-DNA binding domain linked to the LBD/AF-2 domain of hPXR (encoded by plasmid pM-LBDhPXR). pM-LBDhPXR was cotransfected in HepG2 cells in the presence of a GAL4-responsive luciferase reporter gene (plasmid 17mx5-Glob-LUC) with or without increasing amounts of mSHP or hSHP expression plasmids. Reporter expression assay showed that RIF-enhanced luciferase expression only in the presence of pM-LBDhPXR (Fig. 4
). The cotransfection of both mSHP or hSHP expression vectors resulted 1) in a moderate but dose-dependent reduction of pM-LBDhPXR basal transactivation of the reporter, and 2) a stronger decrease of the RIF-induced enhancement of luciferase expression. In addition, inhibitions of LBD-hPXR by hSHP appeared somewhat stronger that those observed with mPXR. These results show that the inhibitory effect of SHP on PXR might be mediated through the LBD/AF-2 domain of PXR.

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Fig. 4. mSHP and hSHP Inhibit GAL4-LBDhPXR-Dependent Transactivation in Transient Transfection Assays
HepG2 cells were cotransfected with 50 ng pM-LBD hPXR with or without increasing amount of mSHP or hSHP expression plasmids (50250 ng) in combination with 200 ng of the 17mx5-ßGlob-LUC containing five GAL4 binding sites upstream of the luciferase reporter gene. pSV-ß-galactosidase (25 ng) was added as internal control, and pM empty vector was added to keep constant the total amount of DNA transfected. Cells were then incubated for 24 h with solvent (NT) or 5 µM RIF, and assayed for LUC and ß-galactosidase activities.
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The AF-2 Coactivator SRC-1 Antagonizes the Inhibitory Effect of SHP on PXR in Mammalian Cells
Recently, it has been proposed that SHP inhibits the ER transcriptional activity by acting as a negative AF-2 coregulator (3, 6, 13). In fact, whereas SHP binds to the ligand activated ER, it does not inhibit ER homodimer DNA binding. GST pull-down and mammalian cell cotransfection experiments revealed that SHP competes with AF-2 coactivators (transcription intermediary factor 2 and receptor-interacting protein with a molecular mass of 140 kDa) for binding to the ligand-activated ER. We therefore decided to test whether such interference between SHP and coactivators could explain our observations on PXR. SRC-1 is a member of the P160/SRC-1 family of positive AF-2 coactivators that bind to the LBD/AF-2 region of ligand-activated nuclear receptors and enhance their transcriptional activity (34). Notably, SRC-1 interacts with PXR (21). Using cotransfection assays in CV1 cells, we first verified that SRC-1 significantly enhances PXR transcriptional activity (Fig. 5
). More interestingly, whereas SHP inhibits PXR-mediated reporter gene expression, the cotransfection of increasing amounts of SRC-1 expression plasmid partially abrogates the inhibitory effect of SHP on the transcriptional activity of PXR and suggest that SHP may act as a negative AF-2 coregulator of PXR.

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Fig. 5. SHP-Mediated Inhibition of Transcriptional Activity of PXR Is Reversed by SRC-1
HepG2 cells were cotransfected with the p(CYP3A4/XREM/-1100/+43)-tk-LUC reporter plasmid (250 ng) together with different combinations of expression plasmids for pSG5- ATG-hPXR, hSHP, and mSRC-1 as indicated with pSV-ß-galactosidase (25 ng) as internal control. Cells were then incubated for 24 h with solvent (NT) or 5 µM RIF, and assayed for luciferase and ß-galactosidase activities.
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Bile Acid-Induced SHP Expression Correlates with the Inhibition of PXR Function in Human Hepatocytes and in Mice
The data presented above collectively show that SHP acts as a repressor of PXR function. As SHP gene expression is regulated by bile acid-activated FXR (4, 9), we next decided to examine the effect of FXR ligands such as CA and CDCA (26), on the PXR-mediated induction of CYP3A in vitro in human hepatocytes (Fig. 6
) and in vivo in mice (Fig. 7
). For this purpose, human hepatocytes were first pretreated for 72 h with increasing concentrations of CDCA (1050 µM) and then treated for 16 h with 5 µM RIF. The level of CYP3A4 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA was examined at the same time by Northern blotting analysis. As expected, RIF provoked an induction of CYP3A4, whereas CDCA had no effect (Fig. 6
, A and B). However, pretreatment of cells with CDCA resulted in a concentration-dependent inhibition of RIF-mediated induction of CYP3A4 mRNA, so that, in the presence of 50 µM CDCA, CYP3A4 mRNA expression was reduced by approximately 70% (n = 3, FT178, FT180, and FT215). As expected, CDCA treatment alone provoked an increase in SHP mRNA expression (5-fold induction) and a total inhibition of CYP7
mRNA expression as shown in Fig. 6
, B and C. RIF-mediated CYP2B6 and CYP2C9 expressions were also decreased by CDCA pretreatment as shown in Fig. 6C
, whereas PXR and CAR expression was not decrease by CDCA treatment. These results, obtained in a fully functional and highly differentiated cellular system, confirm that activation of SHP expression by a bile acid coincides with a strong reduction of PXR-mediated CYP expression. In addition, in CV1 cells cotransfected with the pM-LBDhPXR and a GAL4-responsive luciferase reporter gene, we observed no effect of CDCA nor CA on RIF-mediated luciferase expression (data not shown). These results rule out a possible antagonistic effect of these compounds on PXR.

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Fig. 6. The FXR Ligand CDCA Represses RIF-Mediated PXR Target Genes Expression in Human Hepatocytes
A, Primary human hepatocytes (FT 178 and FT 180) were pretreated 72 h with increasing concentrations of CDCA (10, 25, or 50 µM) and then cultured for 16 h with or without 5 µM RIF. Northern blot analysis was performed onto 20 µg of total RNA using specific CYP3A4 and GAPDH probes. B, Human hepatocytes (FT 215) were pretreated 72 h with increasing concentrations of CDCA (20 or 50 µM) and then cultured for 16 h with or without 5 µM RIF. Northern blot analysis was performed on 20 µg of total RNA using specific CYP3A4, GAPDH, SHP, and CYP7 probes. C, Primary human hepatocytes (FT 178, FT 180, and FT 215) were pretreated 72 h with 50 µM CDCA and then cultured for 16 h with or without 5 µM RIF. cDNAs were prepared from 1 µg of total RNA using reverse transcriptase and a tenth of this was used to determined the levels of CYP2B6, CYP2C9, PXR, CAR, CYP7 , and GAPDH mRNAs by real time RT-PCR analysis using the Light Cycler apparatus (Roche). Data presented are means of the ratio of mRNA levels compared with untreated cells, normalized with respect to GAPDH mRNA levels. ND, Undetectable.
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Fig. 7. CA Represses PXR-Mediated cyp3a11 Expression in Mice
Mice (n = 3) were fed normal chow or chow diets containing 1% CA (wt/wt) for 5 d and PCN (40 mg/kg) was injected in corn oil the last 2 d. Total RNA was isolated from individual livers. Northern blotting analysis were performed onto 30 µg of total RNA using CYP3A, GAPDH, and mSHP probes, quantified by PhosphorImager, and standardized against GAPDH and the results are shown as histogram. Statistically significant relative expressions were determined by Students t test and P values are indicated.
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To determine whether this process occurs in vivo, mice (n = 3) were fed a normal diet or a 1% CA-supplemented diet for 3 d and were then treated for 2 d with PCN (40 mg/kg) or corn oil [untreated (UT)]. Total liver RNA was prepared and analyzed by Northern blotting with the indicated probes (CYP3A and GAPDH were analyzed at the same time, and then the membrane first stripped and then probed with radiolabeled mSHP cDNA). As shown in Fig. 7
, cyp3a11 expression was strongly increased (
14-fold) by PCN. More interestingly, CA pretreatment significantly decreased PCN-mediated cyp3a11 expression (50% inhibition compared with control mice treated with PCN), whereas it moderately induced cyp3a11 expression (
2-fold). As expected, SHP expression was strongly increased by CA pretreatment. Thus, although there is a 14-fold induction of cyp3a11 gene expression by PCN in mice fed a normal diet, this value falls to approximately 3- to 4-fold induction in mice fed a 1% CA-supplemented diet.
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DISCUSSION
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Originally, SHP had been reported to bind to and inhibit CAR (2), a nuclear receptor involved in the induction of the CYP2 and CYP3 genes involved in the metabolism of xenobiotics in response to the prototypical inducer phenobarbital (35). Like CAR, PXR is a nuclear receptor that activates the CYP2 and CYP3A families in response to diverse chemicals and endogenous molecules. In addition, PXR regulates the expression of genes involved in the biosynthesis, transport, and metabolism of bile acids including CYP7
(36), Oatp2 (25), and CYP3A (21). Thus, it has been proposed that PXR serves as a physiological sensor not only for xenobiotics but also for bile acid precursors (22, 23). Our results suggest that SHP is a negative regulator of PXR transcriptional activity. This conclusion derives from in vitro, cell culture, and in vivo experiments.
GST pull-down assays demonstrated a direct interaction between SHP and both murine and hPXR which is enhanced by the presence of PXR ligands such as PCN and RIF, respectively. This interaction is specific as no binding was observed with our internal negative controls (GST alone or luciferase). Transient transfections in different cell lines and with different reporter constructs (i.e. native CYP3A4 promoter or repetitions of the minimal PXR responsive element), demonstrated that expression of SHP produced a dose-dependent inhibition of both murine and hPXR transcriptional activity and that this inhibition was reversed by the expression of SRC-1. This reversibility argues in favor of a functional and specific effect of SHP on PXR, and improves the understanding of the mechanism by which inhibition proceeds. Two complementary mechanisms can thus be proposed: 1) SHP interacts directly with PXR and weakens its binding to DNA as proposed previously for RAR; and 2) SHP blocks the AF-2 activation domain as proposed previously for ER (6).
We speculated that an overexpression of SHP would lead to a decrease in PXR activity and eventually to a decrease in CYP3A inducibility. SHP expression is under the control of FXR, which is activated by primary bile acids such as CA and CDCA (26). We have developed primary cultures of human hepatocytes in which many of the regulatory proteins (including aryl hydrocarbon receptor, CAR, PXR, glucocorticoid receptor, FXR, SHP) are expressed, whereas xenobiotic metabolism and hormonal regulation are fully maintained. As a consequence, the CYPs and others drug metabolizing enzymes are expressed and inducible in these cultures to an extent that is close to the in vivo situation (37, 38). The pretreatment of human hepatocytes with these compounds resulted in a clear overexpression of SHP. This overexpression was confirmed by the concomitant down-regulation of CYP7
, the rate-limiting enzyme in bile acid biosynthesis. Indeed, CYP7
is known to be subject to primary bile acid feedback regulation via SHP (4). In agreement with our hypothesis, PXR activation of CYP3A4, CYP2B6 and CYP2C9 genes was inhibited by FXR activators in a dose-dependent manner. These effects cannot result from a toxicity due to CDCA treatment because both GAPDH and PXR mRNA expression were unaffected, and SHP mRNA level was up-regulated. Moreover, no detectable toxicity on cell culture has been noticed macroscopically (by examination under a microscope) or in terms of total RNA recovery. We finally used mice in an attempt to validate in vivo the observations made in vitro and in cellular models. Indeed, mice fed a CA-enriched diet exhibited a drastic decrease in PCN-induced cyp3a11 expression, whereas SHP was up-regulated.
The conversion of cholesterol to bile acids occurs exclusively in the liver. This process involves the translocation of cholesterol and intermediates through various compartments of the cell, where they encounter a wide variety of enzymes (CYP27A, CYP7
, CYP8B, CYP3A, etc.) necessary for their ultimate conversion to the primary C24 bile acids, CDCA and CA. Many nuclear receptors (liver X receptor, FXR, PXR) control this process through a complex network in which substrates and end-products modulate the up- and down-regulation of specific enzymes. According to our results, PXR function is inhibited in the presence of CDCA or CA through the up-regulation of SHP. This is reminiscent of the SHP-mediated repression of LRH-1 induced CYP7
expression. CYP7
, and to a lower extent CYP3A, promote cholesterol conversion into bile acids. The reason why primary bile acids repress PXR activity and, by consequence, the expression of those genes (including CYP3A) involved in the clearance of bile acids is still unclear. As CA and CDCA are nontoxic bile acid derivatives, in contrast to some bile acids precursors or secondary bile acids products, one explanation is that this process could prevent the over conversion of cholesterol into such toxic compounds through a regulatory feedback loop similar to that observed with CYP7
(Fig. 8
).

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Fig. 8. Model Describing Feedback Regulations of Cholesterol Conversion into Bile Acids by Nuclear Receptors
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The current findings are in agreement with previous observations. Handschin et al. (28) reported that cotreatment with CA or CDCA and phenobarbital or clotrimazole (activators of CAR and PXR, respectively) reduced CYP3A induction in a chicken hepatoma cell line LMH. In addition, Paolini et al. (27) reported that taurochenodeoxycholic acid (a FXR ligand) reduced CYP3A-associated monooxygenase activities in vivo in rats. Moreover, Schuetz et al. (29) observed a strong induction of cyp3a11 and cyp2b10 in FXR -/- mice. Furthermore, in CYP27 -/- mice, where CA and CDCA biosynthesis are abolished, there is an overaccumulation of cyp3a11 and CYP7
compared with wild-type mice (19, 22), whereas feeding of these mice diets containing CA or CDCA lead to up-regulation of SHP and down-regulation of Cyp3a11 or CYP7
(22).
In conclusion, we have shown that SHP interacts with and inhibits the transcriptional activity of PXR. As PXR controls the inducible expression of CYPs and other genes involved not only in the metabolism and elimination of xenobiotics but also in the biosynthesis and catabolism of bile acids, the current results reveal a functional interaction between bile acid homeostasis and the xenobiotic-mediated CYP induction in which SHP appears to play a major role.
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MATERIALS AND METHODS
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Drugs and Materials
DMEM culture medium, CA, CDCA, dimethylsulfoxide (DMSO), pregnenolone 16
-carbonitrile (PCN), RIF, and culture medium additives were from Sigma (Saint Louis, MO).
-(32P)deoxy (d)-ATP and
-(32P)d-CTP were from Amersham International (Amersham, Buckinghamshire, UK).
Plasmids
The following plasmids have been described previously: pCR3-mCAR (39), pSG5-
ATG-hPXR, p(CYP3A4 XREM[-7800/+7200] -1100/+43)-pGL3-LUC (32), pSG5-mPXR.1, pSG5-hPXR and p(CYP3A1 DR3)3-tkCAT (21), CDM8-mSHP (2). pSG5-SRC-1 and GST-SRC-1 (amino acids 580750) are from V. Cavailles, (Institut National de la Santé et de la Recherche Médicale, Montpellier, France). The pCMV-hSHP plasmid was given by Jun Takeda (40). The 17mx5-ßGlob-LUC containing five GAL4 binding sites upstream of the luciferase reporter gene is from P. Chambon (Institut de Génétique Moléculaire et de Biologie Cellulaire, Strasbourg, France). The pM-LBDhPXR expression vector was generated by inserting a PCR fragment corresponding to the +107/STOP amino acids of the hPXR ligand binding domain (LBD) in the pM vector (CLONTECH). GST-mSHP fusion construct was generated by cloning the SmaI/XhoI fragment of the CDM8-mSHP into the EcoIRC1/XhoI sites of the pGEX-4T (Amersham). GST-hPXR fusion construct was generated by inserting the PCR-generated hPXR (amino acids 1431) using oligonucleotides 5'-CCTCAGCTACCTGTGATGCCG and 5'-GGGTGTGGGGAATTCACCACCATGGAGGTGAGACCCAAAGAAAGC primers into pGEX-4T digested by EcoIRC1.
Cell Culture and Transfections
CV1 cells (Monkey kidney) or HepG2 cells (human hepatocarcinoma) were obtained from the American Type Culture Collection (Manassas, VA) and maintained in DMEM supplemented with 10% fetal calf serum, 100 mg/liter penicillin, and 100 mg/liter streptomycin (Life Technologies, Inc., Gaithersburg, MD). Transfection of plasmid DNA was performed in single batches with Fugene-6 (Roche Applied Science, Indianapolis, IN) as instructed by the manufacturer. Transfection was performed using 80,000 cells, and cell extracts were prepared and analyzed for luciferase or CAT and ß-galactosidase activities as described (32).
DNA Binding Assays
A P32-labeled oligonucleotide containing the human CYP3A4 PXRE (ER6, 5'-ATATGAACTCAAAGGAGGTCAGTG) motif was incubated for 20 min at room temperature with 1.5 µl of in vitro synthesized
ATGhPXR, mRXR
(coupled transcription-translation (TNT) reticulocyte lysate system, Promega, Madison, WI), with or without purified GST or GST-mSHP, or 250-fold molar excess of competitor unlabeled ER6 oligonucleotide as previously reported (41). GST and GST-mSHP was purified from bacteria-expressed fusion protein and dialysis against 1x EMSA buffer [10 mM Tris (pH 8.0), 100 mM KCl]. mSHP and GST were stored at 1 mg/ml in 1x EMSA buffer complemented with 10% glycerol. DNA-protein complexes were resolved on a 4% polyacrylamide gel (30:1 acrylamide:bis-acrylamide) in 0.5x TBE (1x TBE = 89 mM Tris, 89 mM boric acid, and 2 mM EDTA). Gels were dried and subjected to autoradiography.
In Vitro Interaction
35S-Methionine-labeled proteins were prepared by in vitro translation using the TNT-coupled transcriptional translation system according to the manufacturers instructions (Promega). GST fusion proteins were expressed in the Escherichia coli BL21 strain and purified using glutathione-sepharose-4B bead affinity chromatography as suggested by the vendor (Pharmacia, Uppsala, Sweden). The beads were subsequently washed and resuspended in 20 mM Tris (pH 8.0), 100 mM NaCl, 0.1% Nonidet P-40 buffer (NETN). GST proteins bound to glutathione-sepharose were incubated with 5 µl of 35S-methionine-labeled proteins in the presence of NETN buffer and 50 µM of indicated compound or 1% DMSO. After overnight incubation at 4 C with gentle agitation, agarose beads were extensively washed with NETN buffer and bound proteins were eluted in sample buffer and analyzed by SDS-PAGE. Gels were then stained with coomassie blue, incubated in an autoradiography enhancer (Dupont NEN, Boston, MA), dried and subjected to autoradiography at -70 C.
Primary Culture of Human Hepatocytes
Hepatocytes were prepared from lobectomy segments resected from adult patients for medically required purposes unrelated to our research program. The use of these human hepatic specimens for scientific purposes has been approved by the French National Ethics Committee. Hepatocytes were prepared and cultured according to the previously published procedure (42). The cells were plated into 60-mm plastic dishes precoated with collagen at 4 x 106 cells per plate in a total volume of 3 ml of a hormonally and chemically defined medium elaborated from a mixture of Williams E and Ham F12 (1:1 in volume). Forty-eight hours after plating, cells were cultured in the presence or absence of CDCA (1050 µM) for 72 h. Cells were then treated with DMSO or 5 µM RIF for 16 h.
Animals and Treatments
Male mice (C57/BL6 from Charles River Laboratories, LArbresle, France, BL3EV05919) were housed in a pathogen-free animal facility under a standard 12-h light, 12-h dark cycle. After 1 wk of acclimatization, mice were fed ad libitum for 3 d with standard rodent chow (diet-1820 (4.5% lipids) Harlan, Ganat, France) or supplemented in house with 1% CA. PCN was injected for two successive daily administrations of 40 mg/kg body wet, in corn oil. The mice were killed 24 h following the last PCN administration.
Total RNA Purification and Northern Blot
Total RNA was extracted from frozen mice liver tissues or human hepatocytes using Trizol reagent (GIBCO BRL, Cergy-Pontoise, France) according to the manufacturers instructions and its purity was confirmed by spectrophotometry. For Northern blot experiments, 30 µg of total RNA were analyzed using
(32P)-dCTP-labeled CYP3A4 and rat GAPDH cDNA probes as previously described (32). Probe for SHP was obtained after SmaI/XhoI digestion and purification of the CDM8-mSHP plasmid. Probe for CYP7
(716 nucleotides) was generated by RT-PCR using the following primers 5'-TCCAGCGACTTTCTGGAGTT and 5'-AAAGGGACTGTGTGGTGAGG (NM_000780). After PCR, the cDNA was cloned into pCR2-TOPO (Invitrogen, Cergy-Pontoise, France) and verified by sequencing. The signals were analyzed by quantifying the radioactivity with a PhosphoImager apparatus and ImageQuant software (both from Molecular Dynamics, Sunnyvale, CA).
Quantitative PCR
Quantification of GAPDH, CYP2B6, CYP2C9, PXR, and SHP mRNA was performed using the Roche Light Cycler apparatus. cDNA were synthesized from 1 µg of total RNA using the Superscript II first-strand synthesis system for PCR (Invitrogen) at 42 C for 60 min, in the presence of random hexamers. One tenth was used for PCR amplification. The following program was used: denaturation step 95 C, 8 min; 45 cycles of PCR (denaturation 95 C, 15 sec; annealing 65 C, 7 sec; elongation 72 C, 19 sec). In all cases, the quality of the PCR-product was assessed by monitoring a fusion step. Sense and reverse primers were as follows, respectively: GAPDH: 5'-GGTCGGAGTCAACGGATTTGGTCG and 5'-CAAAGTTGTCATGGATGACC, CYP2B6: 5'-GGCCATACGGGAGGCCCTTG and 5'-AGGGCCCCTTGGATTTCCG, CYP2C9: 5'-TCCTATCATTGATTACTTCCCG and 5'-AACTGCAGTGTTTTCCAAGC, PXR: 5'-TCCGGAAAGATCTGTGCTCT and 5'-AGGGAGATCTGGTCCTCGAT, SHP: 5'-CCAATGATAGGGCGAAAGAA and 5'-GCTGTCTGGAGTCCTTCTGG, CAR: 5'-CCGTGTGGGGTTCCAGGTAG, and 5'-CAGCCAGCAGGCCTAGCAAC, CYP7
: 5'-CACCTTGAGGACGGTTCCTA and 5'-CGATCCAAAGGGCATGTAGT.
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ACKNOWLEDGMENTS
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We are grateful to Dr. S. Kliewer (GlaxoWellcome, Research Triangle Park, NC) and Dr. M. Negishi (National Institute of Environmental Health Sciences, Research Triangle Park, NC) for providing expression vectors for h- and mPXR, and mCAR, respectively. We are also grateful to Dr. V. Cavailles (U148, Montpellier, France) for providing GST-SRC-1 plasmid and Dr. Jun Takeda (Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan) for providing pCMV-hSHP expression plasmid. We thank Dr. L. Pichard-Garcia for the preparation of human hepatocytes and Colette Bétoulières for assistance in execution of animal studies. We would like to thank Dr. S. Jalaguier (U409, Montpellier France) for critical discussions of this work.
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FOOTNOTES
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This work was supported by grants from the Institut National de la Santé et de la Recherche Médicale, and Pfizer (to J.C.O.).
Abbreviations: AF, Activation function; CA, cholic acid; CAR, constitutive androstane receptor; CDCA, chenodeoxycholic acid; CYP7
, cholesterol 7
-hydroxylase; DBD, DNA-binding domain; DMSO, dimtheylsulfoxide; FXR, farnesoid X receptor; GAL4, positive regulator of galactose inducible genes in yeast; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GST, glutathione-S-transferase; HNF4, hepatocyte nuclear factor-4; h, human; LRH-1, liver receptor homolog-1; m, mouse; mSHP, murine SHP fusion protein; PCN, pregnenolone 16-carbonitrile; PXR, pregnane X receptor; RAR, retinoic acid receptor; RIF, rifampicin; RXR, retinoid X receptor; SHP, small heterodimer partner; SRC-1, steroid receptor coactivator-1; TR, thyroid hormone receptor; UT, untreated; XREM, xenobiotic-responsive element module.
Received for publication November 19, 2002.
Accepted for publication June 4, 2003.
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REFERENCES
|
---|
- Gronemeyer H, Moras D 1995 Nuclear receptors. How to finger DNA. Nature 375:190191[CrossRef][Medline]
- Seol W, Chung M, Moore DD 1997 Novel receptor interaction and repression domains in the orphan receptor SHP. Mol Cell Biol 17:71267131[Abstract]
- Seol W, Hanstein B, Brown M, Moore DD 1998 Inhibition of estrogen receptor action by the orphan receptor SHP (short heterodimer partner). Mol Endocrinol 12:15511557[Abstract/Free Full Text]
- Goodwin B, Jones SA, Price RR, Watson MA, McKee DD, Moore LB, Galardi C, Wilson JG, Lewis MC, Roth ME, Maloney PR, Willson TM, Kliewer SA 2000 A regulatory cascade of the nuclear receptors FXR, SHP-1, and LRH-1 represses bile acid biosynthesis. Mol Cell 6:517526[Medline]
- Gobinet J, Auzou G, Nicolas JC, Sultan C, Jalaguier S 2001 Characterization of the interaction between androgen receptor and a new transcriptional inhibitor, SHP. Biochemistry 40:1536915377[CrossRef][Medline]
- Johansson L, Thomsen JS, Damdimopoulos AE, Spyrou G, Gustafsson JA, Treuter E 1999 The orphan nuclear receptor SHP inhibits agonist-dependent transcriptional activity of estrogen receptors ER
and ERß. J Biol Chem 274:345353[Abstract/Free Full Text]
- Klinge CM, Jernigan SC, Risinger KE, Lee JE, Tyulmenkov VV, Falkner KC, Prough RA 2001 Short heterodimer partner (SHP) orphan nuclear receptor inhibits the transcriptional activity of aryl hydrocarbon receptor (AHR)/AHR nuclear translocator (ARNT). Arch Biochem Biophys 390:6470[CrossRef][Medline]
- Lee YK, Dell H, Dowhan DH, Hadzopoulou-Cladaras M, Moore DD 2000 The orphan nuclear receptor SHP inhibits hepatocyte nuclear factor 4 and retinoid X receptor transactivation: two mechanisms for repression. Mol Cell Biol 20:187195[Abstract/Free Full Text]
- Lu TT, Makishima M, Repa JJ, Schoonjans K, Kerr TA, Auwerx J, Mangelsdorf DJ 2000 Molecular basis for feedback regulation of bile acid synthesis by nuclear receptors. Mol Cell 6:507515[Medline]
- Seol W, Choi HS, Moore DD 1996 An orphan nuclear hormone receptor that lacks a DNA binding domain and heterodimerizes with other receptors. Science 272:13361339[Abstract]
- Brendel C, Schoonjans K, Botrugno OA, Treuter E, Auwerx J 2002 The small heterodimer partner interacts with the liver X receptor
and represses its transcriptional activity. Mol Endocrinol 16:20652076[Abstract/Free Full Text]
- Crawford PA, Dorn C, Sadovsky Y, Milbrandt J 1998 Nuclear receptor DAX-1 recruits nuclear receptor corepressor N-CoR to steroidogenic factor 1. Mol Cell Biol 18:29492956[Abstract/Free Full Text]
- Johansson L, Bavner A, Thomsen JS, Farnegardh M, Gustafsson JA, Treuter E 2000 The orphan nuclear receptor SHP utilizes conserved LXXLL-related motifs for interactions with ligand-activated estrogen receptors. Mol Cell Biol 20:11241133[Abstract/Free Full Text]
- Chiang JY, Kimmel R, Weinberger C, Stroup D 2000 Farnesoid X receptor responds to bile acids and represses cholesterol 7
-hydroxylase gene (CYP7A1) transcription. J Biol Chem 275:1091810924[Abstract/Free Full Text]
- Wang H, Chen J, Hollister K, Sowers LC, Forman BM 1999 Endogenous bile acids are ligands for the nuclear receptor FXR/BAR. Mol Cell 3:543553[Medline]
- Maurel P 1996 The CYP3A family. Boca Raton, FL: CRC Press
- Araya Z, Wikvall K 1999 6
-Hydroxylation of taurochenodeoxycholic acid and lithocholic acid by CYP3A4 in human liver microsomes. Biochim Biophys Acta 1438:4754[Medline]
- Furster C, Wikvall K Identification of CYP3A4 as the major enzyme responsible for 25-hydroxylation of 5ß-cholestane-3
,7
,12
-triol in human liver microsomes. Biochim Biophys Acta 1437:4652
- Honda A, Salen G, Matsuzaki Y, Batta AK, Xu G, Leitersdorf E, Tint GS, Erickson SK, Tanaka N, Shefer S 2001 Side chain hydroxylations in bile acid biosynthesis catalyzed by CYP3A are markedly up-regulated in Cyp27-/- mice but not in cerebrotendinous xanthomatosis. J Biol Chem 276:3457934585[Abstract/Free Full Text]
- Cheng FW, Shefer S, Dayal B, Tint GS, Setoguchi T, Salen G, Mosbach EH 1977 Cholic acid biosynthesis: conversion of 5ß-cholestane-3
,7
,12
,25-tetrol into 5ß-cholestane-3
,7
, 12
,24ß,25-pentol by human and rat liver microsomes. J Lipid Res 18:613[Abstract]
- Lehmann JM, McKee DD, Watson MA, Willson TM, Moore JT, Kliewer SA 1998 The human orphan nuclear receptor PXR is activated by compounds that regulate CYP3A4 gene expression and cause drug interactions. J Clin Invest 102:10161023[Abstract/Free Full Text]
- Goodwin B, Gauthier KC, Umetani M, Watson MA, Lochansky MI, Collins JL, Leitersdorf E, Mangelsdorf DJ, Kliewer SA, Repa JJ 2003 Identification of bile acid precursors as endogenous ligands for the nuclear xenobiotic pregnane X receptor. Proc Natl Acad Sci USA 100:223228[Abstract/Free Full Text]
- Dussault I, Yoo HD, Lin M, Wang E, Fan M, Batta AK, Salen G, Erickson SK, Forman BM 2003 Identification of an endogenous ligand that activates pregnane X receptor-mediated sterol clearance. Proc Natl Acad Sci USA 100:833838[Abstract/Free Full Text]
- Staudinger JL, Goodwin B, Jones SA, Hawkins-Brown D, MacKenzie KI, LaTour A, Liu Y, Klaassen CD, Brown KK, Reinhard J, Willson TM, Koller BH, Kliewer SA 2001 The nuclear receptor PXR is a lithocholic acid sensor that protects against liver toxicity. Proc Natl Acad Sci USA 98:33693374[Abstract/Free Full Text]
- Staudinger J, Liu Y, Madan A, Habeebu S, Klaassen CD 2001 Coordinate regulation of xenobiotic and bile acid homeostasis by pregnane X receptor. Drug Metab Dispos 29:14671472[Abstract/Free Full Text]
- Parks DJ, Blanchard SG, Bledsoe RK, Chandra G, Consler TG, Kliewer SA, Stimmel JB, Willson TM, Zavacki AM, Moore DD, Lehmann JM 1999 Bile acids: natural ligands for an orphan nuclear receptor. Science 284:13651368[Abstract/Free Full Text]
- Paolini M, Pozzetti L, Piazza F, Guerra MC, Speroni E, Cantelli-Forti G, Roda A 2000 Mechanism for the prevention of cholestasis involving cytochrome P4503A overexpression. J Investig Med 48:4959[Medline]
- Handschin C, Podvinec M, Amherd R, Looser R, Ourlin JC, Meyer UA 2002 Cholesterol and bile acids regulate xenosensor signaling in drug-mediated induction of cytochromes P450. J Biol Chem 277:2956129567[Abstract/Free Full Text]
- Schuetz EG, Strom S, Yasuda K, Lecureur V, Assem M, Brimer C, Lamba J, Kim RB, Ramachandran V, Komoroski BJ, Venkataramanan R, Cai H, Sinal CJ, Gonzalez FJ, Schuetz JD 2001 Disrupted bile acid homeostasis reveals an unexpected interaction among nuclear hormone receptors, transporters, and cytochrome P450. J Biol Chem 276:3941139418[Abstract/Free Full Text]
- Kliewer SA, Moore JT, Wade L, Staudinger JL, Watson MA, Jones SA, McKee DD, Oliver BB, Willson TM, Zetterstrom RH, Perlmann T, Lehmann JM 1998 An orphan nuclear receptor activated by pregnanes defines a novel steroid signaling pathway. Cell 92:7382[Medline]
- Goodwin B, Hodgson E, Liddle C 1999 The orphan human pregnane X receptor mediates the transcriptional activation of CYP3A4 by rifampicin through a distal enhancer module. Mol Pharmacol 56:13291339[Abstract/Free Full Text]
- Pascussi JM, Drocourt L, Gerbal-Chaloin S, Fabre JM, Maurel P, Vilarem MJ 2001 Dual effect of dexamethasone on CYP3A4 gene expression in human hepatocytes. Sequential role of glucocorticoid receptor and pregnane X receptor. Eur J Biochem 268:63466358[Abstract/Free Full Text]
- Zanaria E, Muscatelli F, Bardoni B, Strom TM, Guioli S, Guo W, Lalli E, Moser C, Walker AP, McCabe ER, Meitinger T, Monaco AP, Sassone-Corsi P, Camerino G 1994 An unusual member of the nuclear hormone receptor superfamily responsible for X-linked adrenal hypoplasia congenita. Nature 372:635641[CrossRef][Medline]
- Feng W, Ribeiro RC, Wagner RL, Nguyen H, Apriletti JW, Fletterick RJ, Baxter JD, Kushner PJ, West BL 1998 Hormone-dependent coactivator binding to a hydrophobic cleft on nuclear receptors. Sciences 280:17471749[Abstract/Free Full Text]
- Waxman DJ 1999 P450 gene induction by structurally diverse xenochemicals: central role of nuclear receptors CAR, PXR, and PPAR. Arch Biochem Biophys 369:1123[CrossRef][Medline]
- Bock HH, Lammert F 2002 Nuclear xeno-sensors as receptors for cholestatic bile acids: the second line of defense. Hepatology 35:232234[CrossRef][Medline]
- Ferrini JB, PL, Domergue J, Maurel P 1997 Long-term primary cultures of adult human hepatocytes. Chem Biol Interact 107:3145[CrossRef][Medline]
- Ferrini JB, Rodrigues E, Dulic V, Pichard-Garcia L, Fabr JM, Blanc P, Maurel P 2001 Expression and DNA-binding activity of C/EBP
and C/EBPß in human liver and differentiated primary hepatocytes. J Hepatol 35:170177[CrossRef][Medline]
- Honkakoski P, Zelko I, Sueyoshi T, Negishi M 1998 The nuclear orphan receptor CAR-retinoid X receptor heterodimer activates the phenobarbital-responsive enhancer module of the CYP2B gene. Mol Cell Biol 18:56525658[Abstract/Free Full Text]
- Nishizawa H, Yamagata K, Shimomura I, Takahashi M, Kuriyama H, Kishida K, Hotta K, Nagaretani H, Maeda N, Matsuda M, Kihara S, Nakamura T, Nishigori H, Tomura H, Moore DD, Takeda J, Funahashi T, Matsuzawa Y 2002 Small heterodimer partner, an orphan nuclear receptor, augments peroxisome proliferator-activated receptor gamma transactivation. J Biol Chem 277:15861592[Abstract/Free Full Text]
- Drocourt L, Ourlin JC, Pascussi JM, Maurel P, Vilarem MJ 2002 Expression of CYP3A4, CYP2B6, and CYP2C9 is regulated by the vitamin D receptor pathway in primary human hepatocytes. J Biol Chem 277:2512525132[Abstract/Free Full Text]
- Pichard L, Fabre I, Daujat M, Domergue J, Joyeux H, Maurel P 1992 Effect of corticosteroids on the expression of cytochromes P450 and on cyclosporin A oxidase activity in primary cultures of human hepatocytes. Mol Pharmacol 41:104710455[Abstract]