* Sezione di Bioinformatica e Genomica, Istituto Tecnologie Biomediche C.N.R. Bari, Italy
Dipartimento di Scienze Biomolecolari e Biotecnologie. Università di Milano, Milano, Italy
Laboratoire Paleontologie, Universite Sciences and Techniques, Montpellier, France
University of Haifa, Haifa, Israel
|| Dipartimento di Biochimica e Biologia Molecolare, Università di Bari, Bari, Italy
Correspondence: E-mail: graziano.pesole{at}unimi.it.
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Abstract |
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Key Words: mtDNA phylogeny Glires rodent monophyly
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Introduction |
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Genes from the nuclear (Murphy et al. 2001b) and mitochondrial genomes (Arnason and Janke 2002) may produce distinct phylogenies as a result of different inheritance pathways, divergent selection pressures, and differential responses to processes such as lineage sorting, gene duplication/deletion, and hybrid speciation. Conversely, congruent phylogenies among these two genomes could strongly suggest that the gene trees are also congruent with the single underlying phylogeny, the species phylogeny. Therefore, comparison of gene phylogenies of the two genomes will provide an opportunity for robust reconstruction of the complex mammalian phylogeny.
In the light of this background, we have sequenced the complete mitochondrial genome of two rodent species, the blind mole rat (Spalax judaei) (Nevo, Ivanitskaya, and Beiles 2001) and the lesser Egyptian jerboa (Jaculus jaculus), along with a new lagomorph species, the American pika (Ochotona princeps).
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Materials and Methods |
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A total of 70 mammalian species, covering 17 orders of placental mammals, plus Marsupialia and Monotremata as outgroups, were analyzed (table 1). When more mtDNA sequences were available for the same genus, only one species for genus was selected. However, the two available Cynocephalus mtDNA sequences were both included in the analyses data set, because of their remarkable differences at sequence level. The ungapped first and second codon positions of mitochondrial H-stranded protein-coding genes, with the exclusion of leucine synonymous sites in first codon position (Leu-SynP1), were retained for phylogenetic analysis (6,025 nucleotides).
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Maximum-likelihood analysis was carried out using PAUP* using GTR++I model with given model parameters estimated on a neighbor-joining tree. Alternative tree topologies were investigated using the approximately unbiased test, as implemented in the program CONSEL (Shimodaira and Hasegawa 2001).
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Results and Discussion |
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Figure 1 shows a phylogenetic tree with Bayesian posterior probabilities for individual branches. The phylogeny of placental mammals is well resolved and, with the exception of eight nodes, posterior probabilities are higher than 0.85. The results affirm the monophyly of traditional placental orders (except for Primates, Artiodactyla, and Insectivora) and support the supraordinal clades of Afrotheria, Laurasiatheria, and Euarchontoglires, previously proposed based on nuclear gene analyses (Madsen et al. 2001; Murphy et al. 2001a; Murphy et al. 2001b). Apart some minor topological differences (within Cetartiodactyla and Eulipotyphla), the phylogenetic recontruction presented here is substantially congruent with that previously obtained with nuclear genes. A general agreement between mitochondrial and nuclear trees has been previously observed during analyses of mitochondrial rRNA (Jow et al. 2002) and protein genes but only with unrooted trees (Lin et al. 2002; Lin, Waddell, and Penny 2002). Indeed, the mitochondrial placental tree was different from the nuclear one when marsupial and/or monotreme outgroups were used (Lin et al. 2002; Lin, Waddell, and Penny 2002).
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Within Euarchontoglires, our results support the position of Scandentia as outgroup and Dermoptera as sister group of the Anthropoidea, which makes Primates polyphyletic in agreement with previous results obtained on mitochondrial data (Arnason et al. 2002). Nuclear data have not resolved this issue (Murphy et al. 2001a) and does not support the monophyly of Primates and a sister relationship between Dermoptera and Scandentia using a limited taxon sampling (Murphy et al. 2001b). The polyphyletic Primates are highly supported (PP = 100) by both nucleotide and protein trees. Indeed, Primates monophyly was significantly rejected for (Primates [Dermoptera, Scandentia]) and (Dermoptera [Primates, Scandentia]) topologies (P < 0.05 in AU test). Only a (Scandentia [Primates, Dermoptera]) topology could not be significantly rejected by the AU test (P = 0.056).
Within Laurasiatheria, the only striking difference with molecular phylogenies based on nuclear data is the different positions of alpaca and pig. Pig emerges basal to other Cetartiodactyla in both nucleotide (fig. 1) and protein tree (not shown), whereas the alpaca is more closely related to the clade that includes hippo and Cetacea in the nucleotide tree (fig. 1) and basal with respect to other Cetartiodactyla (excluding pig) in the protein tree (not shown). However, the nuclear topology with a basal alpaca cannot be rejected by the AU test (P = 0.103).
One of the most debated issues among morphologists, paleontologists, and molecular biologists is the position of Rodentia within the mammalian tree and the relationship among the major lineages of Rodentia. The monophyly of Rodentia and Glires as well as the position of the latter as a sister group of Primates is significantly supported for the first time by mitochondrial genes (fig. 1) in both the nucleotide (PP = 100 [see fig. 1]) and the protein tree (PP = 86 [data not shown]). The observed rodent monophyly and the support for the Glires clade is in clear contrast with previous mitochondrial surveys, which showed rodent paraphyly (Reyes, Pesole, and Saccone 1998; Reyes et al. 2000; Reyes, Pesole, and Saccone 2000; Arnason et al. 2002 [but see Philippe 1997; Sullivan and Swofford 1997]). This is most likely caused by the inclusion of new rodent mitochondrial genomes that would break the long branches leading to Muridae (Reyes, Pesole, and Saccone 2000) rather than to the exclusion of leucine synonymous sites from the data set and the use of the Bayesian phylogenetic method. Indeed, rodent monophyly is maintained if leucine synonymous sites are included or the phylogenetic tree is reconstructed using the maximum-likelihood method (data not shown). Such results (fig. 1) are in perfect agreement with those obtained from nuclear gene trees (Madsen et al. 2001; Murphy et al. 2001a; Murphy et al. 2001b) and with morphological and paleontological data (Carroll 1988; Luckett and Hartenberger 1993).
Regarding the relationships among rodent families, our data support the clustering of Gliridae with Sciuridae and a Caviomorpha plus Phiomorpha clade. Muridae, Spalacidae, and Dipodidae are placed in a different clade (fig. 1). The separation of rodents into these clades is in agreement with previous results based on complete mitochondrial genomes (Reyes, Pesole, and Saccone 1998; Reyes et al. 2000; Reyes, Pesole, and Saccone 2000; Arnason et al. 2002). Nuclear genes show either a polytomy for the major lineages of rodents or Muridae clustering with Caviomorpha and Phiomorpha, leaving Sciuridae and Gliridae as the most divergent groups (Madsen et al. 2001; Murphy et al. 2001a; Murphy et al. 2001b; Huchon et al. 2002). Traditional morphological classifications also provide a different view of relationships among rodent families: Gliridae is the closest relative to the clade containing Dipodidae plus Spalacidae and Muridae, then Sciuridae, and finally Caviomorpha together with Phiomorpha (McKenna and Bell 1997). However, other morphological and paleontological surveys provide evidence for the close relationship between Gliridae and Sciuridae (Bugge 1985; Lavocat and Parent 1985; Hartenberger 1996), giving further support to our data. None of these alternative tree topologies based on nuclear, morphological, or paleontological data can be rejected by our data (P > 0.05 in AU tests). This suggests that taxon sampling among the numerous families of rodents might not be extensive enough to discriminate between the best tree and the alternative topologies, thus highlighting the need for more comprehensive sampling.
The other controversial point is the position of lagomorphs within the mammalian tree and, in particular, their association with rodents in the cohort Glires. Our results highly support the clustering of Lagomorpha and Rodentia in Glires (fig. 1). Based on morphology, Glires has always been considered as a monophyletic group with well-defined characters (Hartenberger 1996; Archibald, Averianov, and Ekdale 2001), but this view has been frequently challenged by molecular studies, based on both nuclear and mitochondrial genes. In these studies, lagomorphs appeared as a clade branching off before the split between Primates and Ferungulata or as a sister clade of Euarchonta (Graur, Duret, and Gouy 1996; Reyes, Pesole, and Saccone 1998; Reyes et al. 2000; Reyes, Pesole, and Saccone 2000; Arnason et al. 2002). Both hypotheses were rejected by our data based on mitochondrial genomes (P < 0.01 in AU tests). Recent surveys based on complete mitochondrial genomes had suggested a close relationship between Lagomorpha and certain families of Rodentia (Reyes et al. 2000; Reyes, Pesole, and Saccone 2000), but it has been mainly by means of nuclear genes that the existence of Glires has been highly supported (Madsen et al. 2001; Murphy et al. 2001a; Murphy et al. 2001b; Huchon et al. 2002). Our study strongly supports the existence of Glires, probably as consequence of a more comprehensive sampling of both Lagomorpha and Rodentia.
The fourth superordinal lineage, Laurasiatheria, includes the remaining placental orders: Carnivora, Perissodactyla, Cetartiodactyla, Chiroptera, and core Insectivora (fig. 1). The clustering of these orders has received support from previous surveys based on both complete mitochondrial genomes (Reyes, Pesole, and Saccone 1998; Reyes et al. 2000; Reyes, Pesole, and Saccone 2000; Arnason et al. 2002) and nuclear genes (Madsen et al. 2001; Murphy et al. 2001a; Murphy et al. 2001b). The monophyly of Chiroptera, which has been a controversial issue over the last decade (Pettigrew 1986), is well supported by our data. The polyphyly of Eulipotyphla, with Soricomorpha and Erinaceomorpha forming a paraphyletic group, is also well supported by our data (fig. 1), but a misplacement because of the presence of some residual compositional bias in Erinaceomorpha cannot be excluded.
Bayesian phylogenetic reconstruction has provided remarkable resolving power, with only 12% of the nodes in the tree (8/68) showing PP < 0.85. Although it is likely that Bayesian approach results in overcredibility of some nodes (Suzuki, Glazko, and Nei 2002; Alfaro, Zoller, and Lutzoni 2003; Douady et al. 2003)and this also emerges from some AU tests presented herewe are confident of the general reliability of the obtained topology. This derives from the observation that ML reconstruction, under the GTR++I model, carried out on the same data (not shown), was fully topologically congruent with Bayesian reconstruction. The utility of mitochondrial DNA for phylogenetic reconstruction is based on the fact that the mitochondrial genes are inherited as a single nonrecombining linkage unit and thus do not provide independent estimates of the species tree. In contrast, because nuclear genes are usually selected randomly from different chromosomes, each gene tree may provide an independent estimate of the species tree. In addition, the effective population size of mitochondrial genes is smaller than that of nuclear genes as a consequence of their different mode of inheritance. Because it is known that effective population size has a great impact in the accuracy of phylogenetic reconstruction, specially between successive bifurcations, mitochondrial genes tree have a better chance of tracking the species tree than nuclear gene (Moore 1995). In this sense, contrasting perspectives have been obtained in different surveys regarding the merits of nuclear and mitochondrial genes for recovering phylogenetic information. One of them states that mitochondrial genes would be more efficient in recovering resolved mammalian phylogenies and that only with a much higher number of nuclear genes similar results could be obtained (Arnason, Gullberg, and Janke 1999). By contrast, the other perspective claims that if sequence length is comparable, nuclear genes have a greater resolving power than mitochondrial genes (Springer et al. 2001). However, our study shows that mitochondrial and nuclear genes are equally reliable for recovering order-level and family-level relationships among eutherian mammals when comprehensive taxon sampling is used, compositional bias is taken into account, and robust methods are used for tree reconstruction.
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Acknowledgements |
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Footnotes |
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