*Department of Biological Sciences, University of South Carolina;
Department of Cell and Molecular Biology, Roswell Park Cancer Institute
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Abstract |
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Introduction |
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1-Proteinase inhibitor (
1-PI; also known as
1-antitrypsin) is a 50-to 55-kDa glycosylated plasma protein that is synthesized primarily in the liver and represents one of the more abundant serpins in the mammalian bloodstream. The central function of
1-PI in humans is the inhibition of neutrophil elastase (Potempa, Korzus, and Travis 1991; Wright 1996
). The importance of this inhibitory function is underscored by the existence of the Z allele of the
1-PI gene, which encodes a mutant protein that undergoes polymerization within the endoplasmic reticulum of liver cells and is therefore not secreted. This results in a severe deficiency of the inhibitor in the bloodstream, excessive degradation of elastin fibers in the lung, and eventually, pulmonary emphysema (Crystal 1989
; Le et al. 1992
; Lomas et al. 1992, 1993
).
The number of 1-PI genes differs among mammalian species. Primates, sheep, bovine, rat, and some mouse species contain a single gene, whereas guinea pigs, rabbits, gerbils, and other mouse species contain as many as five (Borriello and Krauter 1991
; Rheaume et al. 1994
; Goodwin, Baumann, and Berger 1996
; Goodwin, Barbour, and Berger 1997
). Phylogenetic considerations suggest that the small gene families in the latter species were formed by amplification events that took place at separate times during evolution. The
1-PIs in mice probably arose just before the rat-mouse split (i.e., about 1525 MYA) and underwent changes in number during subsequent evolution of the Mus genus. This led to the emergence of variation in the size of the
1-PI family within and among murine species (Borriello and Krauter 1991
; Rheaume et al. 1994
; Goodwin, Baumann, and Berger 1996
; Goodwin, Barbour, and Berger 1997
).
The amino acid sequence of the RCL of 1-PIs, like that of other serpins, is highly divergent within and among species, particularly in the region between P6 and P1'. A number of studies have indicated that RCL hypervariability has been driven by positive Darwinian selection, whereby the high rates of amino acid substitution have resulted in adaptive functional alterations (Carrell, Pemberton, and Boswell 1987
; Hill and Hastie 1987
; Laskowski et al. 1987
; Borriello and Krauter 1991
; Inglis and Hill 1991
; Rheaume et al. 1994
; Goodwin, Baumann, and Berger 1996
; Goodwin, Barbour, and Berger 1997
). This predicts that the biochemical function, i.e., the target specificities, of the various
1-PIs are distinct. Recent evidence indicates that only two of the five
1-PIs in mice bind and inhibit elastase (Paterson and Moore 1996
). However, the role of the RCL in such a isoform-specific function remains to be addressed.
In the current study, we demonstrate that individual mouse 1-PI isoforms in two mouse species differ with regard to their ability to bind serine proteinases, indicating that they have distinct biochemical functions. Furthermore, we show that the RCL is a primary determinant of such differences, which are likely to be a consequence of positive selection acting upon this region of the
1-PI polypeptide. We discuss several possible physiological roles of
1-PI diversity.
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Materials and Methods |
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Constructs for Expression of 1-PI Isoforms
Complementary DNAs corresponding to 1-PIs in the C57BL/6J and BALB/cJ strains of Mus musculus domesticus and in the wild-derived species Mus saxicola were isolated from appropriate liver cDNA libraries (Rheaume et al. 1994
). All cDNAs, which were approximately 1.3 kb in length, spanned the full length of the
1-PI mRNA molecule, from the 5'-untranslated region to the poly(A) tail. Each was isolated as an EcoRI fragment and was subcloned into expression vector pSVSportI (GIBCO BRL) or pcDNAI (Invitrogen).
Chimeric 1-PIs containing the RCL region of one isoform in the place of the homologous region of another were generated using the M. saxicola cDNAs. The 1.3-kb cDNAs were subcloned as EcoRI fragments into a derivative of pBS-SK(-) from which the BamHI and SmaI restriction sites were deleted by mutagenesis. The 242-bp BamHI-SmaI fragment of the donor cDNA, which corresponds to the RCL region between residues 328 and 408, was cloned into the recipient cDNA from which the homologous BamHI-SmaI fragment had been removed. The resulting chimeric cDNA was cut out of pBS-SK(-) with EcoRI and subcloned into pSVSportI or pcDNAI.
Transfection of COS-1 Cells
The 1-PIexpressing plasmids were introduced into COS-1 cells by transient transfection. Cells cultured in 15 cm dishes in DMEM containing 10% heat-inactivated fetal calf serum were transfected by the DEAE-dextran method (Lopata, Cleveland, and Sollner-Webb 1984
). After a 24-h recovery period, the cells were washed three times with phosphate-buffered saline and incubated for an additional 24 h in serum-free DMEM containing 4.5 mg/ml glucose; the high glucose concentrations were required to maintain glycosylation of
1-PI polypeptide chains (Baumann and Jahreis 1983
). Conditioned medium containing the secreted
1-PI was collected, and the cell debris was removed by brief centrifugation.
Purification of Recombinant 1-PIs
The conditioned medium was diluted in two volumes H2O, adjusted to a pH of 7.8, and applied to a 1-ml DEAE-Sepharose column (Pharmacia) equilibrated in 50 mM Tris-HCl, pH 7.5. The column was washed thoroughly with 50 mM Tris-HCl, pH 7.5, followed by a brief wash with 10 ml of buffer containing 100 mM NaCl; bound 1-PI was eluted with buffer containing 150 mM NaCl. Fractions containing the
1-PI were pooled and loaded onto a 0.5 x 30 cm Superose 200 column (Pharmacia) equilibrated with 50 mM Tris-HCl, pH 7.8, 150 mM NaCl. The inhibitor eluted in a single peak at the expected molecular weight range of approximately 50 kDa. Accurate quantitation of
1-PI concentrations in pooled peak fractions was done by one- and two-dimensional polyacrylamide gel electrophoresis, together with known amounts of the purified plasma-derived inhibitor; Coomassie bluestained proteins were quantitated by densitometry, and the results were confirmed by Western blotting. We estimate that the
1-PI concentrations in the unprocessed conditioned medium of transfected cells range between 1 and 10 µg/ml.
The relative abundance of recombinant 1-PIs, along with their degrees of size and charge microheterogeneity, were assessed by incubating cells in serum-free medium containing 100 µCi/ml [35S]methionine (>1,000 Ci/mmol; Amersham) and analyzing 10 µl aliquots of the medium by two-dimensional PAGE (1.5 x 120 x 160 mm; Hoeffer); radiolabeled
1-PIs were visualized by fluorography.
Immunodetection of free and proteinase-bound 1-PI was carried out by Western blotting, using a polyclonal sheep antiserum raised against purified mouse
1-PI as probe; the antiserum was provided by Dr. J. Gauldie (McMaster University, Hamilton, Ontario, Canada). Antigen was detected by enhanced chemiluminescence (Amersham).
Determination of 1-PI Binding to Serine Proteinases
Recombinant 1-PI isoforms were assayed for their ability to covalently bind each of several serine proteinases. Elastase binding was assayed in reaction mixtures containing the inhibitor (110 ng), 8 µl binding buffer (0.1 M Tris-HCl, pH 8.0, 0.5 M NaCl, 20 mM CaCl2), and 1 µl human neutrophil elastase (180 ng; Elastin Products, Orensville, Mo.). Mixtures were incubated at 37°C for 5 min and stopped by the addition of 2 µl 15 mM Na2EDTA and 100 ng N-MeOSuc-Ala-Ala-Pro-Val-chloromethylketone (Sigma). An equal volume of SDS sample buffer was added, and the mixtures were loaded onto 7.5% SDS PAGE gels. Free- and proteinase-bound
1-PIs were visualized by Western blotting, as described previously.
Binding of 1-PI to other serine proteinases (chymotrypsin, trypsin, and cathepsin G) was assayed in a similar fashion, except that the binding buffer was 50 mM Tris-HCl, pH 7.8, 150 mM NaCl, and 20 mM CaCl2. Reaction mixtures contained 12.5200 ng bovine pancreatic
-chymotrypsin (Type VII, TLCK treated; Sigma), 25400 ng bovine pancreatic trypsin (Type XIII, TPCK treated; Sigma), or 15240 ng human leukocyte cathepsin G (Sigma). Mixtures were incubated for 5 min at 37°C, SDS sample buffer was added, and samples were subjected to SDS PAGE and Western blotting to detect free- and proteinase-bound
1-PIs.
1-PI Binding to Snake Venom Proteinases
Lyophilized, unfractionated venoms were obtained from several snake species. Venoms from the Russell's viper (Vipera russelli) and Gabon viper (Bitis gabonica) were purchased from Sigma, whereas those from the Malayan pit viper (Agkistrodon rhodostoma), Rhombic night adder (Causus rhombeatus), sand viper (Vipera ammodytes), and Barba amarilla (Bothrops atrox) were obtained from the Miami Serpentarium Laboratories and were provided to us by Dr. L. Kress (Roswell Park Cancer Institute, Buffalo, N.Y.). To detect covalent complexes between 1-PIs and proteinases in the venoms, the venom samples were dissolved in 150 mM NaCl, 0.001 N HCl to a protein concentration of 50 mg/ml and immediately diluted 10-fold in 50 mM Tris-HCl, pH 7.8, 150 mM NaCl, 20 mM CaCl2. Aliquots containing 0.180 µg venom protein were used to measure covalent
1-PI binding to proteinases.
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Results |
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Northern blot analysis of mouse liver RNA has indicated that the levels of most 1-PI transcripts are relatively constant during postnatal development and adult life; however, two mRNAs, i.e., those encoding DOM-5 and DOM-7 in M. m. domesticus, are sexually dimorphic. Figure 1
shows that the concentrations of the DOM-5specific and DOM-7specific mRNAs are higher in males than in females. Expression of mRNAs encoding the other isoforms is not significantly different between the sexes (fig. 1
and data not shown). Both the DOM-5 and DOM-7 mRNAs are induced in females after treatment with testosterone (data not shown), indicating that
1-PI expression is modulated by androgens.
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Two-dimensional gel electrophoresis indicated that 1-PI is a major secreted protein of transfected COS-1 cells. Figure 2
shows representative gels containing proteins secreted from cells expressing SAX-1, -3, or -4. Each
1-PI exists as a characteristic series of proteins that exhibit microheterogeneity with regard to molecular weight and isoelectric point. This microheterogeneity spans the ranges expected for native murine
1-PI. These proteins were not observed in control, mock-transfected cells (fig. 2
). Structural studies involving glycosidase digestion (data not shown) have indicated that the secreted
1-PIs contain, like the native inhibitor, as many as four N-linked oligosaccharide units. Similar results were obtained using
1-PI cDNAs encoding DOM-1, -2, -3, -4, and -6 (data not shown).
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Recognition of Serine Proteinases in Snake Venoms
The experiments described in the preceding section made use of commercially available serine proteinases derived from a variety of sources. These may or may not represent the physiologically relevant targets in the healthy, intact mouse. It is of interest to consider proteinases from a more natural context, such as those in snake venom. It has been postulated that RCL hypervariability within serpin molecules broadens the spectrum of proteinases recognized and inhibited by 1-PIs, providing protection against exogenous proteinases introduced by predators or pathogens (or both; Hill and Hastie 1987
). Accordingly, we predicted that various
1-PI isoforms should differ in recognition of proteinases in snake venom, which represents a complex mixture of serine proteinases, as well as other classes of proteolytic enzymes. Specific
1-PI isoforms may bind and inhibit some venom proteinases, while being degraded by others.
To test this prediction, we measured the ability of individual 1-PIs to covalently bind proteinases in crude venoms from each of several snake species. Aliquots of the venoms were incubated with SAX-1, -3, or -4, and the reaction mixtures were subjected to Western blot analysis. As seen in figure 6
, the SAX-1 isoform entered into a covalent complex with one or more proteinase(s) in each of the two Vipera venoms and formed a distinct complex with B. gabonica venom. SAX-1 formed no detectable complex with C. rhombeatus venom and was degraded without appreciable complex formation by venoms from A. rhodostoma and B. atrox (fig. 6
). SAX-3 formed a complex with proteinase(s) in the venom of V. ammodytes but not V. russelli or B. gabonica (fig. 6
). Finally, SAX-4 bound proteinase(s) in venom from V. russelli but not V. ammodytes or B. gabonica (fig. 6
). We conclude that there are striking differences among the three isoforms with regard to recognition of snake venom proteinases. The identities of the venom proteinases that interact with the various isoforms have not been determined. That
1-PIs can be an inhibitor for some venom proteinases and a substrate for others indicates that the total level of proteinase activity at a given site of venom introduction is determined by the balance between substrate and inhibitory activities of the serpin.
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A similar set of experiments was done to assess the role of the RCL in the functional differences between SAX-1 and SAX-3. The 242-bp RCL-containing fragment of SAX-1 was inserted into SAX-3, generating SAX-3-1; conversely, the RCL region of SAX-3 was inserted into SAX-1, generating SAX-1-3. As shown in figure 7 , SAX-3-1, like SAX-1, bound elastase, whereas SAX-1-3, like SAX-3, was inactive. Thus, similar to what was observed in comparing SAX-1 and SAX-4, differences in elastase binding between SAX-1 and SAX-3 are caused by the RCL region.
The differential abilities of SAX-1 and SAX-4 to bind either trypsin or chymotrypsin were also analyzed. SAX-1-4, like SAX-4, formed a complex with trypsin, yet failed to react with chymotrypsin (fig. 7
); conversely, SAX-4-1, like SAX-1, complexed with chymotrypsin, yet exhibited no reaction with trypsin (fig. 7
). These results show that, as with elastase binding, the differential abilities of SAX-1 and SAX-4 to bind trypsin and chymotrypsin are caused by the RCL-containing regions of the inhibitors. One or more amino acid substitutions in this region of the 1-PI polypeptide confers functional differences to the various
1-PI isoforms.
We observed that SAX-3-1 retained the ability to form a complex with chymotrypsin, whereas SAX-1-3 did not (fig. 7 ). This was rather unexpected because both SAX-1 and SAX-3 are able to bind this proteinase (fig. 5 ). The inability of SAX-1-3 to bind chymotrypsin indicates that an intramolecular incompatibility exists between the RCL of SAX-3 and one or more other regions within SAX-1, suggesting that sequences outside the RCL-containing region between residues 368 and 391 influence the ability of the inhibitor to recognize chymotrypsin. Further studies will be necessary in order to understand the mechanism(s) underlying this observation.
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Discussion |
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Functional diversification of serpins has been observed in organisms other than mice. Five serpins in wheat grain, classified into two subfamilies, have been shown to have distinct RCL sequences and proteinases specificities (Østergaard et al. 2000
). In the tobacco hornworm (Manduca sexta), the serpin-1 gene encodes a family of serpin isoforms with distinct RCL regions (Jiang et al. 1996
). These isoforms, which arise as a consequence of alternative splicing of a single gene, exhibit diverse inhibitory profiles toward mammalian, fungal, and microbial proteinases (Jiang and Kanost 1997
). It is likely that the presence of functionally diverse serpins, generated by a variety of mechanisms, is important in a wide range of organisms.
It is to be emphasized that the analytical techniques used in the present report provide a qualitative, rather than a quantitative, comparison of the various 1-PI isoforms. Extensive kinetic studies of serpin binding to proteinases, which are beyond the scope of the present work, will be required to obtain a more quantitative perspective. Regardless, the current results clearly indicate that murine
1-PIs exhibit functional differences that derive from variation within the RCL, providing strong support for the role of positive Darwinian selection in
1-PI evolution.
The physiological relevance of altered target specificities brought on by evolutionarily derived amino acid substitutions within the RCL is not known. The primary in vivo function of 1-PI, i.e., protection of the extracellular matrix against excessive proteolytic degradation, particularly in the lung, derives from its ability to inhibit neutrophil elastase (Potempa, Korzus, and Travis 1994
; Wright 1996
). However, there is evidence to suggest that the serpin plays one or more roles that are independent of elastase inhibition (Nagai et al. 1992
). Furthermore, several
1-PI isoforms lack a methionine residue at the P1 site and do not inhibit elastase (Suzuki et al. 1991
; see figs. 4 and 5 ). It seems clear, therefore, that altered proteinase specificities conferred by the rapidly evolving RCL must have unrecognized functions.
We suggest three possible physiological advantages provided by RCL divergence.
(1) Defense against exogenous parasites, predators, or pathogens: As has been discussed by others (Hill and Hastie 1987
; Borriello and Krauter 1991
; Goodwin, Baumann, and Berger 1996
), RCL hypervariability may confer more efficient defense against exogenous proteinases introduced by parasites, predators, or pathogens. This resistance could occur through changes in the spectrum of target proteinases recognized by the inhibitor, a notion consistent with the experiments presented in the current report. Alternatively, reductions in the sensitivity of the RCL to cleavage and inactivation by exogenous proteinases that are not targets for the inhibitor may arise. An interesting example of this is the opossum, which is resistant to rattlesnake venom as a consequence of production of a novel protein called oprin, which is a potent inhibitor of venom metalloproteinases. Furthermore, although the RCLs of mouse and human
1-PIs are particularly susceptible to cleavage by several snake venom metalloproteinases, the RCL of opossum
1-PI is resistant to such cleavage (Cantanese and Kress 1993
). Production of oprin and resistance of
1-PI to cleavage together probably contribute to the animal's rather remarkable ability to tolerate snake venom (Cantanese and Kress 1992
).
(2) Regulation of inflammation: Several studies indicate that 1-PI, like a number of hepatic plasma proteins that are induced during the acute phase response, acts as an anti-inflammatory agent (Tilg et al. 1993
; Tilg, Dinarello, and Mier 1997
). In vitro,
1-PI has been shown to inhibit superoxide production by human neutrophils (Bucurenci et al. 1992
) and protects cultured lung epithelial cells from the effects of endotoxins (Tumen et al. 1988
). Administration of
1-PI in vivo decreases bleomycin-induced fibrosis in the hamster lung (Nagai et al. 1992
). It is possible that the anti-inflammatory activities of
1-PI may be enhanced or broadened (or both) through diversity in proteinase recognition generated by RCL hypervariability, thereby providing a physiological benefit to the organism.
(3) Protection against carcinogenesis: Proteolytic enzymes are well-known mediators of tumorigenesis because of their ability to degrade and modify components of the extracellular matrix with which tumor cells interact (DeClerck et al. 1997
; Werb 1997
). Maspin, a serpin produced in normal mammary gland, suppresses the growth and metastasis of breast carcinoma cells (Zou et al. 1994
; Sager et al. 1997
). Deficiencies in matrilysin, a matrix metalloproteinase that efficiently cleaves the RCL of
1-PI in vitro (Sires et al. 1994
; Zhang et al. 1994
), results in significant reductions of the tumor burden in mice predisposed to intestinal cancer (Wilson et al. 1997
). It is possible that
1-PI is an antitumorigenic agent whose function is to protect the organism against the multitude of carcinogenic substances (e.g., plant secondary metabolites) encountered within its environment. Variation within the RCL may broaden the spectrum of tumor growth-promoting proteinases that are recognized by the inhibitor, thereby increasing the breadth or efficiency of its anticarcinogenic activity. Our unpublished observations indicating that cleavage and inactivation of
1-PI accompanies tumor development in mice (K. Barbour, unpublished data) is consistent with the notion that the serpin inhibits tumorigenesis.
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Acknowledgements |
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Footnotes |
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1 Present address: Department of Developmental Biology and Anatomy, School of Medicine, University of South Carolina
Address for correspondence and reprints: Franklin G. Berger, Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208. berger{at}sc.edu
.
2 Nomenclature for amino acids within the RCL is according to Schechter and Berger (1967).
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References |
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