* INSERM E0339, Faculté de Médecine Xavier Bichat, Paris, France
UMR CNRS-IRD 9926, Centre IRD, Montpellier, France
Centre d'Etude du Polymorphisme Humain, Hôpital Saint Louis, Paris, France
Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, Paris, France
Correspondence: E-mail: denamur{at}bichat.inserm.fr.
![]() |
Abstract |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Key Words: Escherichia coli bacterial evolution virulence phylogeny
![]() |
Introduction |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
In an effort to investigate the relationship between the genetic background and the virulence genes, we established the phylogenetic relationship of 98 E. coli/Shigella strains representing the pathogenic diversity of the species and determined the presence of different pathogenic determinants. In addition, the evolutionary histories of some of these pathogenic determinants were compared with the strain phylogeny to assess the single or multiple arrivals of such determinants.
![]() |
Materials and Methods |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
A total of 98 E. coli/Shigella strains selected from previously reported collections of prototypes of the diversity of the different pathovars and commensal strains were considered in this study. Strains isolated in pathogenic conditions include 10 EAEC, 16 DAEC, 11 STEC (with nine EHEC), six EPEC, eight ETEC, nine ExPEC, five Shigella/EIEC, and two nonclassified diarrheagenic E. coli. Thirty-one commensal strains were also analyzed. The 30 strains of the ECOR collection (Ochman and Selander 1984) included in this study have been previously used in a phylogenetic study based on the sequence analysis of 11 genes (Escobar-Páramo et al., 2004). The 15 DEC strains are representatives of different intestinal pathogenic groups (ETEC, EPEC 1 and 2, EAEC, and EHEC 1 and 2) previously described by Whittam et al. (1993), Reid, Betting and Whittam (1999), and Reid et al. (2000). Strains EIEC85b, SB01-97, SS92a, SB11-56, and SD01-77 have been selected from a study on the evolution of Shigella/EIEC (Escobar-Páramo et al. 2003), and each one represents a monophyletic group showing the diversity of Shigella. Strains C1845 (isolated from a patient with diarrhea), IH11128 and EC7372 (isolated from urine of patients with UTI) are archetypes of DAEC strains producing Afa/Dr adhesins. The other DAEC strains, isolated from patients with diarrhea from Brazil (seven strains) and France (eight strains), represent the genetic diversity of this pathovar based on the hybridization with 10 probes derived from strain C1845 (Blanc-Potard et al. 2002). In addition, six DAEC strains isolated from asymptomatic patients in Brazil and France (DAECT2, T437, T179, T192, T14, and T19) were included. Strains EAEC 042, JM221, 17-2, and 55989 are reference strains that have been used to elucidate the pathogenicity of enteroaggregative E. coli. Additional strains were selected from different collections: EAEC strains 56390, 384P, 381A, 11097, and 11074; ETEC strains E2539-C1, EDL1493, TX-1, 469, 440, and H10407; EHEC/STEC strains EDL931, 255/1-1, 248/1-2, and H-19; and EPEC strain 2348/69. Strains EDL933, RIMD0509952, CFT073, and RS218 correspond to strains from which the complete genome sequence have been determined. EDL933 and RIMD0509952 are EHEC O157:H7 strains, whereas CFT073 and RS218 are ExPEC strains. A strain of E. fergusonii (ATCC35469T), which is the closest species to E. coli (Lawrence, Ochman and Hartl 1991), was used as the outgroup in the phylogenetic analysis. Specific information on each strain is available on table S1 in Supplementary Material online.
Gene Sequencing
Sequences of six essential chromosomal genes encoding the tryptophan synthase subunits A and B (trpA and trpB) of the tryptophan operon, the p-aminobenzoate synthase (pabB), the proline permease (putP), the isocitrate dehydrogenase (icd), and the polymerase PolII (polB) were obtained for phylogenetic reconstruction of the 99 strains described above. These genes have been shown to exhibit low levels of horizontal gene transfer in E. coli (Lecointre et al. 1998; Denamur et al. 2000) and, thus, are useful to assess strain phylogeny. ECOR sequences, as well as the sequences of the Shigella/EIEC strains and of E. fergusonii, were determined elsewhere (Escobar-Páramo et al. 2003; 2004). Sequences of EDL933, RIMD0509952, CFT073, and RS218 were from the E. coli genome sequence projects. Gene sequences of the remaining 58 strains were obtained by direct sequencing of PCR products as in Bjedov et al. (2003).
In addition, sequences of three VFs representing each category defined below (see Results and Discussion) were performed. hlyCA (505 bp) from hly operon, afaD (550 bp) from the afa operon, coding for an invasin and LT-I (614 bp) sequences were obtained by direct sequencing of PCR products. Sequences of all the used primers are available on table S2 in Supplementary Material online.
Virulence Factors
The presence of 17 VFs, characteristic of the different E. coli pathovars was determined in the entire collection of strains by standard hybridization protocols using digoxigenin (DIG) or radioactive 32P-labeled probes. Determinants highly common in ExPEC strains include the outer membrane usher protein gene papC of the pyelonephritis-associated pilus system, sfa/focDE encoding S fimbrial adhesins, hlyCA genes encoding synthesis of active intracellular -hemolysin, and the cytotoxin necrotizing factorencoding gene cnf1. Two genes involved in iron uptake, chuA and iucC, were also analyzed. Probes afaBC/daaC and M030, specific to the subclass of DAEC strains encoding Afa/Dr adhesins, were also used. The presence of aggregative adhesion-encoding plasmid in EAEC was detected using the classical CVD432 probe (AA probe). The presence of the pathogenicity island containing the locus of enterocyte effacement (LEE) of EHEC and EPEC was evidenced with a probe specific for the eae gene coding for the outer membrane protein intimin. The gene coding for the pilin protein (bfpA) in the bundle-forming pilus (BFP) system of EPEC was used to determine the presence of the EPEC adherence factor (EAF) plasmid (pB171). We also looked at the presence of the heat stable toxin (STa) and the heat labile toxin (LT-I) defining typical ETEC pathovars as well as the Shigalike toxins (stx1 and stx2) of STEC/EHEC. In addition, we determined the presence of the 60-MDa plasmid defining typical EHEC by means of the gene ehxA coding for the enterohemolysin (of the RTX family). Finally, the ipaB gene, a gene of the type III secretion system of Shigella and EIEC encoded by the virulence plasmid, was determined. The sequences of the primers used for generating the PCR probes for hybridations were taken from the literature.
To assign the ExPEC VFs to specific PAIs, additional analyses were performed. The presence of iroN and hra genes coding for an enterobactin siderophore receptor protein and a heat-resistant agglutinin, respectively, was determined by PCR on strains exhibiting at least one ExPEC VF in the first step screening. papG alleles and S fimbrial adhesin types (sfa or foc) were determined by PCR in papC or sfa/foc positive strains, respectively. Sequences of the primers are available on table S2 in Supplementary Material online. The type of intimin allele was taken from the literature (Reid et al. 1999) or determined in silico for the RIMD0509952 strain.
Phylogenetic Analyses
Sequences were aligned using the Clustal program (Higgins, Bleasby, and Fuchs 1992) from the Sequence NavigatorTM package. Neighbor-joining analyses were performed using the BioNJ method of PAUP* version 4.0 (Swofford 2002). The semistrict consensus trees, as well as the bootstrap trees, were obtained using maximum parsimony as the optimality criteria, with the heuristic search of PAUP* 4.0 with 1,000 iterations. The starting tree for the analyses was constructed via stepwise addition with the TBR branch-swapping algorithm. Maximum-likelihood and Bayesian analyses were performed using the PHYML (Guindon and Gascuel 2003) and MrBayes version 2.01 (Huelsenbeck and Ronquist 2001) programs, respectively.
![]() |
Results |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
|
|
The distribution pattern of the pathovars does not always coincide with the distribution of the VFs (fig. 2). For example, in contrast to the localized distribution of ExPEC strains in groups B2 and D, the ExPEC VFs, although more frequent in B2 and D, can be found all over the phylogeny, in both commensal and diarrheic strains. This is the same case for Afa/Dr and M030, the VFs typical of DAEC strains, which are found in other pathovars (STEC, EHEC, and ExPEC) and in commensal strains. However, the presence of toxins (ST, LT, stx1, and stx2) and other VFs (ehxA and ipaB) of strains causing severe diarrhea, as well as the AA gene of EAEC strains, is exclusive to the particular pathovars that harbor these determinants (except strain H10407, an ETEC carrying the AA gene).
The two iron-uptake genes, iucC and chuA, are not associated to a particular pathovar. Nevertheless, whereas iucC is found in all genetic backgrounds, the distribution of chuA is restricted to phylogenetic groups B2, D, E, and SD01, regardless of the pathogenic nature of the strains, which support its value as a phylogenetic marker (Clermont, Bonacorsi, and Bingen 2000).
The Virulence Factor Evolutionary Histories
To test whether the correlation between genetic background and the presence of VFs is due to a single arrival of the VF, we study the evolutionary history of some of the VFs.
The phylogenetic histories of the ExPEC VFs were assessed in two ways. First, we determined their localizations on known PAIs. It has been shown that the simultaneous presence of certain ExPEC VFs and papG alleles could be characteristic of PAI type (Bingen-Bidois et al. 2002; Bonacorsi et al. 2003). Thus, based on the specific combination of pap (including the papG allele), sfa/foc, hly, cnf1, aer, iroN, and hra, we were able to assign the ExPEC VFs to three known PAIs (ICFT073, IIJ96, and III536) in the 22 strains exhibiting such genes (table S3 in Supplementary Material online). As multiple insertion sites have been described for the PAI IIJ96 (Bingen-Bidois et al. 2002), we also analyzed them in some representative strains exhibiting this PAI. Second, because the hly operon can be present within several PAIs or alone (or within an uncharacterized PAI), we chose this gene as a representative of ExPEC VFs to reconstruct its evolutionary history by sequencing hlyCA. Sequencing of hlyCA revealed that some strains exhibit two genes, as previously reported (Swenson et al. 1996; Dobrindt et al. 2002a). Indeed, the presence of two genes was suspected by the observation of two peaks in the same position on the electrophoregram. The individual full sequences were reconstructed (haplotypes) according to the known unique sequences. It is clear that the tree obtained with the hly sequences is clearly incongruent (i.e., in disagreement) with the strain phylogeny tree (fig. 3A). The arrival of the hly operon within the E. coli species corresponds to at least three different events, as hlyCA can be located on the PAI ICFT073 or IIJ96 or alone. The PAIs are not specific to phylogenetic groups: (1) the same PAIs harboring unique hlyCA sequences can be found in diverged strains (PAI IIJ96 in ECOR51 B2 and DAECT179 C strains, PAI ICFT073 in CFT073 and EC7372 B2 and 17-2 A strains) and (2) within the B2 group strains, hly gene can be found alone, in PAI ICFT073 or in PAI IIJ96 at two insertion sites, thus, corresponding at least to four arrival events (fig. 3A). All these data indicate multiple horizontal gene transfers for the acquisition of ExPEC VFs and demonstrate that the evolutionary histories of the VFs are distinct from the history of the strain.
|
The evolutionary history of a gene highly restricted to specific phylogenetic groups was analyzed by sequencing the LT-I gene. The analyzed 614 bp of this gene are highly conserved, as only four mutations (three being nonsynonymous) were observed, one being informative for parsimony (data not shown). This indicates several recent arrival of this gene within the A, B1, and C phylogenetic group strains. The multiple independent arrivals of the LEE PAI harboring eae gene in EPEC have been previously established (Reid et al. 2000).
![]() |
Discussion |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
The link between phylogeny and ExPEC strains has been evidenced in several occasions by different authors (Picard et al. 1993; Boyd and Hartl 1998a; Bingen et al. 1998; Johnson et al. 2001; Bingen-Bidois et al. 2002; Bonacorsi et al. 2003). These studies have shown that the majority of strains isolated from urine or newborn cerebrospinal fluid belong to phylogenetic groups B2 and D and that these strains harbor a greater number of ExPEC VFs as compared with strains from other phylogenetic groups isolated in UTI or NBM. Additionally, it has been shown that the number of ExPEC VFs on a strain is proportional to its pathogenic potential (Picard et al. 1999). Based on our results, there is no evidence that gain of one VF increases the likelihood of retention of another VF (data not shown), as it has been suggested for Salmonella (Ochman and Groisman 1996). Commensal strains from group B2 also seems to harbor more ExPEC VFs than their counterparts from other phylogenetic groups (Duriez et al. 2001; Zhang, Foxman, and Marrs 2002). Two hypotheses have been proposed to explain the concentration of ExPEC VFs within group B2: (1) the existence of preexisting features of the B2 genome that increase compatibility between the B2 genome and ExPEC VFs and, (2) chance and timing with the acquisition of ExPEC VFs by a B2 group ancestor with subsequent vertical inheritance or loss of the VFs (Johnson et al. 2000). Our data (fig. 3A), as well as the sequencing data of papA (Boyd and Hartl 1998b; Johnson et al. 2001) and sfaA (Boyd and Hartl 1998b) in the ECOR strains, demonstrate that the arrival of ExPEC VFs within the phylogenetic B2 group strains correspond in fact to numerous horizontal gene transfer events. Such repeatedly observed recombination events into the same genetic background argue most strongly for B2 genetic background being a critical player in the acquisition of ExPEC VFs. It has been shown, for example, that the efficient expression of the -hemolysin determinant located on a PAI depends on a complex mechanism where several core chromosomal gene products as Hha, H-NS, RfaH, and tRNA5Leu are required (Dobrindt et al. 2002b). One could easily imagine that polymorphisms in sequence or expression of these regulatory proteins will influence the expression of the VF.
The link between phylogeny and virulence is also observed among intestinal pathogens. As mentioned above, toxin-producing and/or enteroinvasive pathovars, such as EHEC, ETEC and Shigella/EIEC, as well as their specific VFs, are only found in groups A, B1, C, or E. The distribution of these VFs is not because of a lack of genetic exchange among these intestinal pathogens and strains from groups D and B2. In fact, analysis of the number of transferred fragments on the sequences of the six essential genes used in the phylogenetic analysis (Denamur et al. 2000) indicates that genetic exchange occurs between strains of different phylogenetic groups, commensal and pathogenic strains, and strains of different pathovars (O. Tenaillon, personal communication). In contrast, it is indicative of the lack of compatibility between these VFs and the D and B2 genetic background.
EHEC strains are mostly concentrated within group E, where strains of serotype O157:H7 are found (EHEC 1 complex [Reid et al. 2000]). There is evidence in support of a model in which O157:H7 evolved sequentially from an O55:H7 (DEC5d) ancestor (Whittam et al. 1993; Feng et al. 1998; Monday, Whittam, and Feng 2001). Our phylogeny does not contradict this scenario, as the position of the DEC5d strain within the E group is not supported by a significant bootstrap value (fig. 1), and individual gene phylogenies showed that DEC5d strain is basal according to the EHEC O157:H7 strains in three of five genes (pabB, icd, and putP; considering trpA and B linked) (data not shown). Interestingly, the ECOR37 strain, which has been isolated from a marmoset, seems to have secondarily lost stx genes. No particular phylogenetic clustering of ETEC strains is observed on the tree (fig. 1), but the distribution of the ST and LT genes (fig. 2) associated with these pathovars is limited to the groups A, B1, and C. This may indicate a predominantly horizontal mode of transmission of these VFs by the multiple independent arrivals of the plasmids carrying these genes from another species or may indicate within-species dissemination. The almost complete nucleotide conservation of the LT-I genes within diverged strains of A, B1, and C phylogenetic groups argues for this scenario of several recent arrivals. However, it seems that an A, B1, or C genetic background is necessary for the arrival and/or maintenance of these genes. The importance of the genetic background on the evolution of Shigella/EIEC has been suggested by the correlation between the lack of some phenotypic characters (such as motility, lysine decarboxylation, and lactose utilization) and the presence of the virulence plasmid responsible for the pathogenic nature of the bacteria (Maurelli et al. 1998; Pupo, Lan, and Reeves 2000; Escobar-Páramo et al. 2003). It has been demonstrated that cadaverine, the product of the activity of the lysine decarboxylase, blocks the action of Shigella enterotoxin (Maurelli et al. 1998) and prevents the escape of S. flexneri from the phagolysosome (Fernandez et al. 2001).
The overall distribution of pathovars associated to mild and chronic diarrhea may be explained by the plasticity of these factors to adapt to different genetic backgrounds or by the interaction of these VF to an "ancestral background" of the bacterial genome. An ancient unique arrival of the VFs associated with these pathovars can be ruled out as the afa sequence data clearly suggest multiple horizontal gene transfer events (fig. 3B).
![]() |
Conclusion |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
This classification of the VFs genetic background interaction allows us to postulate the existence of two types of genomic backgrounds inside the E. coli genome. On one hand, there is the "ancestral background," which is present in all strains and allows the expression of VFs associated with mild and chronic diarrhea. On the other hand, a more "derived background" allows the expression of VFs associated with more severe pathologies. Interestingly, strains associated with severe and acute diarrheas all belong to groups originated after the differentiation of group D, suggesting that an important change in the E. coli genome took place at this point in the evolution of the species. Further modifications occurred after the split of group E, when the virulence plasmid of Shigella/EIEC arrived in the population, giving raise to a radiation from which groups A, B1, and C originated (Escobar-Páramo et al. 2003). Those modifications in the genomic background allow new VFs to arrive in the population, giving origin to the pathovars associated with severe acute diarrheas.
Comparative genomics among strains from different phylogenetic groups may help identify the changes that occurred in the genome after the split of group D (the "derived background"), as well as what may constitute the "ancestral background" of the E. coli genome. These comparisons are necessary for further understanding the implications of the genetic background in the evolution of pathogenicity in bacteria.
![]() |
Supplementary Material |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
![]() |
Acknowledgements |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
![]() |
Footnotes |
---|
![]() |
Literature Cited |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Bingen, E., B. Picard, N. Brahimi, S. Mathy, P. Desjardins, J. Elion, and E. Denamur. 1998. Phylogenetic analysis of Escherichia coli strains causing neonatal meningitis suggests horizontal gene transfer from a predominant pool of highly virulent B2 group strains. J. Infect. Dis. 177:642-650.[ISI][Medline]
Bingen-Bidois, M., O. Clermont, S. Bonacorsi, M. Terki, N. Brahimi, C. Loukil, D. Barraud, and E. Bingen. 2002. Phylogenetic analysis and prevalence of urosepsis strains of Escherichia coli strains bearing pathogenicity islands-like domains. Infect. Immun. 70:3216-3226.
Bjedov, I., G. Lecointre, O. Tenaillon, C. Vaury, M. Radman, F. Taddei, E. Denamur, and I. Matic. 2003. Polymorphism of gene encoding SOS polymerases in natural populations of Escherichia coli. DNA Repair 2:417-426.[CrossRef][ISI][Medline]
Blanc-Potard, A.-B., C. Tinsley, I. Scaletsky, C. Le Bouguenec, J. Guignot, A. L. Servin, X. Nassif, and M.-F. Bernet-Camard. 2002. Representational difference analysis between Afa/Dr diffusely adhering Escherichia coli and non-pathogenic E.coli. K-12. Infect. Immun. 70:5503-5511.
Bonacorsi, S., O. Clermont, V. Houdoin, C. Cordevant, N. Brahimi, A. Marecat, C. Tinsley, X. Nassif, M. Lange, and E. Bingen. 2003. Molecular analysis and experimental virulence of French and North American Escherichia coli neonatal meningitis isolates: identification of a new virulent clone. J. Infect. Dis. 187:1895-1906.[CrossRef][ISI][Medline]
Boyd, E. F., and D. L. Hartl. 1998a. Chromosomal regions specific to pathogenic isolates of Escherichia coli have a clustered distribution. J. Bacteriol. 180:1159-1165.
Boyd, E. F., and D. L. Hartl. 1998b. Diversifying selection governs sequence polymorphism in the major adhesin proteins FimA, PapA, and SfaA of Escherichia coli. J. Mol. Evol. 47:258-267.[ISI][Medline]
Brando, S. Y., G. R. F. do Valle, M. B. Martinez, L. R. Trabulsi, and C. A. Moreira-Filho. 1998. Characterization of enteroinvasive Escherichia coli and Shigella strains by RAPD analysis. FEMS Microbiol. Lett. 165:159-165.[CrossRef][ISI][Medline]
Clermont, O., S. Bonacorsi, and E. Bingen. 2000. Rapid and simple determination of the Escherichia coli phylogenetic groups. Appl. Environ. Microbiol. 66:4555-4558.
Czeczulin, J. R., T. S. Whittam, I. R. Henderson, F. Navarro-Garcia, and J. P. Nataro. 1999. Phylogeneic analysis of enteroaggregative and diffusely adherent Escherichia coli. Infect. Immun. 67:2692-2699.
Denamur, E., G. Lecointre, and P. Darlu, et al. (12 co-authors). 2000. Evolutionary implications of the frequent horizontal transfer of mistmatch repair genes. Cell 103:711-721.[ISI][Medline]
Dobrindt, U., G. Blum-Oehler, N. Gabor, G. Schneider, A. Johann, G. Gottschalk, and J. Hacker. 2002a. Genetic structure and distribution of four pathogenicity islands (PAI I536 to PAI IV536) of uropathogenic Escherichia coli strain 536. Infect. Immun. 70:6365-6372.
Dobrindt, U, L. Emody, I. Gentschev, W. Goebel, and J. Hacker. 2002b. Efficient expression of the alpha-haemolysin determinant in the uropathogenic Escherichia coli strain 536 requires the leuX-encoded tRNA5Leu. Mol. Genet. Genomics 267:370-379.[CrossRef][ISI][Medline]
Donnenberg, M. S. 2002. Escherichia coli: virulence mechanisms of a versatile pathogen. Elsevier Science Edition, Academic Press, San Diego, Calif.
Duriez, P., O. Clermont, S. Bonacorsi, E. Bingen, A. Chaventré, J. Elion, B. Picard, and E. Denamur. 2001. Commensal Escherichia coli isolates are phylogenetically distributed among geographical distinct human populations. Microbiology 147:1671-1676.
Escobar-Páramo, P., C. Giudicelli, C. Parsot, and E. Denamur. 2003. The evolutionary history of Shigella and enteroinvasive Escherichia coli revised. J. Mol. Evol. 57:140-148.[CrossRef][ISI][Medline]
Escobar-Páramo, P., A. Sabbagh, P. Darlu, O. Pradillon, C. Vaury, G. Lecointre, and E. Denamur. 2004. Decreasing the effects of horizontal gene transfer on bacterial phylogeny: the Escherichia coli case study. Mol. Phylogenet. Evol. 30:243-250.
Feng, P., K. A. Lampel, H. Karch, and T. S. Whittam. 1998. Genotypic and phenotypic changes in the emergence of Escherichia coli O157:H7. J. Infect. Dis. 177:1750-1753.[CrossRef][ISI][Medline]
Fernandez, I. M., M. Silva, R. Schuch, W. A. Welker, A. M. Siber, A. T. Maurelli, and B. A. McCormick. 2001. Cadaverine prevents the escape of Shigella flexneri from the phagolysosome: a connection between bacterial dissemination and neutrophil transepithelial signaling. J. Infect. Dis. 184:743-753.[CrossRef][ISI][Medline]
Garcia, M. I., M. Jouve, J. P. Nataro, P. Gounon, and C. Le Bouguénec. 2000. Characterization of the AfaD-like family of invasins encoded by pathogenic Escherichia coli associated with intestinal and extra-intestinal infections. FEBS Lett. 479:111-117.[CrossRef][ISI][Medline]
Gordon D. M., and A. Cowling. 2003. The distribution and genetic structure of Escherichi coli in Australian vertebrates: host and geographic effects. Microbiology 149:3575-3586.
Guignot, J., J. Breard, M. F. Bernet-Camard, I. Peiffer, B. J. Nowicki, A. L. Servin, and A. B. Blanc-Potard. 2000. Pyelonephritogenic diffusely adhering Escherichia coli EC7372 harboring Dr-II adhesin carries classical uropathogenic virulence genes and promotes cell lysis and apoptosis in polarized epithelial caco-2/TC7 cells. Infect. Immun. 68:7018-7027.
Guindon, S., and O. Gascuel. 2003. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst. Biol. 52:696-704.[CrossRef][ISI][Medline]
Hacker, J., and J. B. Kaper. 2000. Pathogenicity island and the evolution of microbes. Ann. Rev. Microbiol. 54:641-679.[CrossRef][ISI][Medline]
Herzer, P. J., S. Inouye, M. Inouye, and T. S. Whittman. 1990. Phylogenetic distribution of branched RNA-linked multicopy single-stranded DNA among natural isolates of Escherichia coli. J. Bacteriol. 172:6175-6181.[ISI][Medline]
Higgins, D. G., A. J. Bleasby, and R. Fuchs. 1992. CLUSTALV: improved software for multiple sequence alignment. CABIOS 8:189-191.[Medline]
Houdouin, V., S. Bonacorsi, N. Brahimi, O. Clermont, X. Nassif, and E. Bingen. 2002. A uropathogenicity island contributes to the pathogenicity of Escherichia coli strains that cause neonatal meningitis. Infect. Immun. 70:5865-5869.
Huelsenbeck, J. P., and F. Ronquist. 2001. MrBayes: Bayesian inference of phylogeny. Bioinformatics 17:754-755.
Johnson, J. R., P. Delavari, M. Kuskowsi, and A. L. Stell. 2001. Phylogenetic distribution of extraintestinal virulence-associated traits in Escherichia coli. J. Infect. Dis. 183:78-88.[CrossRef][ISI][Medline]
Johnson, J. R., M. Kuskowski, E. Denamur, J. Elion, and B. Picard. 2000. Clonal origin, virulence factors, and virulence. Infect. Immun. 68:424-425.
Labigne-Roussel, A., and S. Falkow. 1988. Distribution and degree of heterogeneity of the afimbrial-adhesin-encoding operon (afa) among uropathogenic Escherichia coli isolates. Infect Immun. 56:640-648.[ISI][Medline]
Lawrence, J. G., H. Ochman, and D. L. Hartl. 1991. Molecular and evolutionary relationships among enteric bacteria. J. Gen. Microbiol. 137:1911-1921.[ISI][Medline]
Lecointre, G., L. Rachdi, P. Darlu, and E. Denamur. 1998. Escherichia coli molecular phylogeny using the incongruence length difference test. Mol. Biol. Evol. 15:1685-1695.
Maurelli, A. T., R. E. Fernandez, C. A. Bloch, and C. K. Rode. 1998. "Black holes" and bacterial pathogenicity: a large genomic deletion that enhances the virulence of Shigella spp. and enteroinvasive Escherichia coli. Proc. Natl. Acad. Sci. USA 95:3943-3948.
Monday, S. R., T. S. Whittam, and P. C. H. Feng. 2001. Genetic and evolutionary analysis of mutations in the gusA gene that causes the absence of beta-glucoronidase activity in Escherichia coli O157:H7. J. Infect. Dis. 184:918-921.[CrossRef][ISI][Medline]
Nataro, J. P., and J. B. Kaper. 1998. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11:142-201.
Ochman, H., and E. A. Groisman. 1996. Distribution of pathogenicity islands in Salmonella spp. Infect. Immun. 64:5410-5412.[Abstract]
Ochman, H., J. G. Lawrence, and E. A. Groisman. 2000. Lateral gene transfer and the nature of bacterial innovation. Nature 405:299-304.[CrossRef][ISI][Medline]
Ochman, H., and R. K. Selander. 1984. Standard reference strains of Escherichia coli from natural populations. J. Bacteriol. 157:690-692.[ISI][Medline]
Picard, B., J. S. Garcia, S. Gouriou, P. Duriez, N. Brahimi, E. Bingen, J. Elion, and E. Denamur. 1999. The link between phylogeny and virulence in Escherichia coli extraintestinal infection. Infect. Immun. 67:546-553.
Picard, B., C. Journet-Mancy, N. Picard-Pasquier, and P. Goullet. 1993. Genetic structures of the B2 and B1 Escherichia coli strains responsible for extra-intestinal infections. J. Gen. Microbiol. 139:3079-3088.[ISI][Medline]
Pupo, G. M., D. K. R. Karaolis, R. Lan, and P. R. Reeves. 1997. Evolutionary relationship among pathogenic and nonpathogenic Escherichia coli strains inferred from multilocus enzyme electrophoresis and mdh studies. Infect. Immun. 65:2985-2692.
Pupo, G. M., R. Lan, and P. R. Reeves. 2000. Multiple independent origins of Shigella clones of Escherichia coli and convergent evolution of many of their characters. Proc. Natl. Acad. Sci. USA 97:10567-10572.
Reid, S. D., D. J. Betting, and T. S. Whittam. 1999. Molecular detection and identification of intimin alleles in pathogenic Escherichia coli by multiplex PCR. J. Clin. Microbiol. 37:2719-2722.
Reid, S. D., C. J. Herbelin, A. C. Bumbaugh, R. K. Selander, and T. S. Whittam. 2000. Parallel evolution of virulence in pathogenic Escherichia coli. Nature 406:64-67.[CrossRef][ISI][Medline]
Russo, T. A., and J. R. Johnson. 2000. Proposal for a new inclusive designation for extraintestinal pathogenic isolates of Escherichia coli: ExPEC. J. Infect. Dis. 181:1753-1754.[CrossRef][ISI][Medline]
Swenson, D. L., N. O. Bukanov, D. E. Berg, and R. A. Welch. 1996. Two pathogenicity islands in pathogenic Escherichia coli J96: cosmid cloning and sample sequencing. Infect. Immun. 64:3736-3743.[Abstract]
Swofford, D. L. 2002. PAUP*: phylogenetic analysis using parsimony (*and other methods). Version 4.0. Sinauer Associates, Sunderland, Mass.
Whittam, T. S., M. L. Wolfe, I. K. Wachsmuth, F. Orskov, I. Orskov, and R. A. Wilson. 1993. Clonal relationship among Escherichia coli strains that cause hemorrhagic colitis and infantile diarrhea. Infect. Immun. 61:1619-1629.[Abstract]
Zhang, L., B. Foxman, and C. Marrs. 2002. Both urinary and rectal Escherichia coli isolates are dominated by strains of phylogenetic group B2. J. Clin. Microbiol. 40:3951-3955.