Laboratoire dImmunogénétique Moléculaire, Université Paul Sabatier, Pavillon Charles Lefebvre, Hôpital Purpan, Toulouse, France
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Abstract |
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Introduction |
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Nonhuman primates express counterparts of the human Rh antigens (Masouredis, Dupuy, and Elliot 1967
; Moor-Jankowski and Wiener 1972
). The use of monoclonal antibodies confirmed that chimpanzees and gorillas express polymorphic antigens, namely chimpanzee Rc and gorilla Dgor, which share epitopes with the human D antigen (Socha and Ruffié 1990
; Blancher, Calvas, and Ruffié 1992
). The expression of antigens Rc and Dgor was shown to depend on RH-like genes of chimpanzees and gorillas, respectively (Salvignol et al. 1993, 1994
; Blancher and Socha 1997
). Gibbons and orangutans also express D-like antigens, but the small number of animals studied did not allow the description of a polymorphism in these species (Blancher and Socha 1997
). It has to be noted that if the expression of Rh-like antigens seems to be restricted to apes, the presence of Rh-like polypeptides at the surfaces of RBCs of Old World monkeys, New World monkeys, lemurs, and many other mammalians (cats, dogs, bovines, rats, mice) was evidenced by biochemical techniques (Saboori, Denker, and Agre 1989
) or by immunoblotting (Mouro et al. 1994
; Salvignol et al. 1995
; Apoil and Blancher 1999
). Despite their conservation throughout evolution, the function of Rh polypeptides remains elusive.
Southern blot studies demonstrated that only humans and African apes (chimpanzees and gorillas) possess more than one gene per haploid genome (Salvignol et al. 1993
; Westhoff and Wylie 1994
; Blancher and Socha 1997
). Chimpanzees and gorillas possess at least three and two RH-like genes, respectively (Blancher and Socha 1997
). Thus, it was deduced that the duplication event which led to the appearance of the RHCE and RHD genes arose most probably in the common ancestor species of humans, chimpanzees, and gorillas.
The two human RH genes differ in their coding sequences, but also in their noncoding elements, including length polymorphisms of intron 3 (Matassi et al. 1997
) and intron 4 (Arce et al. 1993
). Intron 4 of the RHCE gene differs from intron 4 of the RHD gene in the presence of a 654-bp fragment which encompasses an Alu-Sx element (Westhoff and Wylie 1996
; Huang 1997b;
Okuda et al. 1997
). Like humans, gorilla possesses introns 3 and 4 length polymorphisms which are counterparts of the RHD/RHCE intronic polymorphisms observed in humans (Westhoff and Wylie 1996
; Apoil, Roubinet, and Blancher 1999
). For example, some gorillas RH-like genes exhibit RHCE-like introns 4 which harbor an Alu-Sx element orthologous to the Alu in human RHCE intron 4 (Apoil, Roubinet, and Blancher 1999
). It was not possible from previous results to determine whether the Alu insertion in the RHCE gene arose after the duplication of a common ancestor gene or whether the RHD ancestor gene lost a 654-bp fragment. Results presented here are in good agreement with the deletion hypothesis.
Although chimpanzees and gorillas possess more than one gene per haploid genome (Blancher, Calvas, and Ruffié 1992
), comparative studies of chimpanzee and gorilla RH-like cDNAs did not bring a definitive demonstration that genetic exchanges have occurred between RH-like genes in these species (Salvignol et al. 1995
; Apoil and Blancher 1999
). With the aim of demonstrating such intergenic exchanges, we decided to study noncoding parts of chimpanzee and gorilla RH-like genes.
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Materials and Methods |
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Amplification and Sequencing of Introns 4 ofRH-like Genes
Primers complementary to exons 4 or 5 of RH-like genes were deduced from the Rh cDNA nucleotide sequences previously characterized (Mouro et al. 1994
; Salvignol et al. 1995
; Apoil and Blancher 1999
). The sequences of primers were Ex.4-dir1 (CGATACCCAGTTTGTCTGCCATGC), Ex.5-rev (TTGGGGTGAGCCAAGGATGAC(C/A)C), Ex.4-dir2 (AGCCTATTTTGGCTGACTG), Ex.4.cons-dir (GCCTATTTTGGGCTGACTGTGG), and Ex.5.cons-rev (GGCCAGAATATCCACAAGAAGAG).
The primer pairs Ex.4-dir1/Ex.5-rev, Ex.4-dir2/Ex.5-rev, and Ex.4.cons-dir/Ex.5.cons-rev were used for the amplification of RH-like introns 4 in humans and African apes, Old World monkeys and New World monkeys, and mice and lemurs, respectively. Thirty amplification cycles were carried out using an enzyme mix of Taq and Pwo DNA polymerases (Expand High Fidelity, Boerhinger-Mannheim, Indianapolis, Ind.). Purified PCR products were sequenced by using the fluorescent dye terminator cycle sequencing method (PE Applied Biosystems, Foster City, Calif.). When required, amplified fragments were cloned into pCR 2.1.TOPO plasmid vector (TOPO TA Cloning kit, Invitrogen, Leek, the Netherlands) and sequenced.
Length Polymorphism of Primate RHD-like Introns 3 and 4
Primers Int.3-dir ((A/G)GGATTACAAGCAAGC-ATCACC) and Int.3-rev (CACGCAC(C/T)TCACT-GATTCCTACTTC) were deduced from the intron 3 sequences of human RH genes (Matassi et al. 1997
). This pair of primers led to the amplification of 580-bp (RHCE intron 3) or 290-bp (RHD intron 3) fragments with human genomic DNA. This set of primers was used to test for the presence of length polymorphism in the 3' part of RH-like gene introns 3 in chimpanzees and gorillas. The pair of primers Int.4.cons-dir (CTCCCTCCT-TTACCAA(C/G)TTC) and Int.4.cons-rev (AATCTGCATACCCCAGGC) allowed the amplification of a short segment (140 bp) of RHD-like introns 4 under the following conditions: 25 cycles of PCR (denaturation for 15 s, annealing for 20 s at 55°C, and extension for 10 s) were carried out using 1.25 U of Taq DNA polymerase (QIAGEN, Hilden, Germany) in a reaction medium supplemented with 20% of Q-solution additive (QIAGEN).
Association of Introns 3 and 4 in Chimpanzee and Gorilla RH-like Genes
Primers RB46-dir (TGGCAAGAACCTGGACCTTGACTTT) (Matassi et al. 1997
) and Ex.5-rev were used to amplify DNA fragments encompassing intron 3 to the 5' part of exon 5 from genomic DNA samples of one chimpanzee and two gorillas. These three animals were selected because they possessed introns 3 of the RHCE and RHD-like types and introns 4 of RHD-like type 1 and type 2. Amplicons recovered from agarose gel after electrophoresis were partially sequenced. Sequences of segments surrounding the intron 3 RHD-specific deletion were characterized, as were regions encompassing exon 4, intron 4, and a part of exon 5 (see fig. 1
for details).
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Phylogenetic Analysis
Intron 4 nucleotide sequences were aligned using CLUSTAL W, version 1.7 (Thompson, Higgins, and Gibson 1994
). Phylogenetic analysis was carried out with the MEGA software package (Kumar, Tamura, and Nei 1993
). Rates of substitutions (K values) were calculated according to Kimuras (1980)
two-parameter method, and trees were reconstructed by the neighbor-joining method using the pairwise deletion option. One thousand resampled versions of the original data set were generated by bootstrap, and the 1,000 corresponding trees were deduced by the neighbor-joining method.
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Result |
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Like human RHD intron 4, other primate intron 4 sequences exhibited a deletion of a region encompassing the Alu element and 354 bp 5' of the Alu element. For that reason, these primate introns 4 were called RHD-like. In the chimpanzee and the gorilla, two types of RHD-like intron 4 sequence were defined by the absence (RHD-like type 1) or the presence (RHD-like type 2) of a 12mer repeat 3' of the deleted region (fig. 3 ).
The brown lemur and mouse RH introns 4 are also deprived of an Alu element and are thus presented aligned with the human RHCE intron 4 sequence with its Alu-Sx element deleted (fig. 4 ). This alignment confirms the insertion point of the Alu-Sx element in intron 4 of the RH gene ancestor common to Anthropoidea. The mouse Rh intron 4 is 356 bp long, and its sequence was too divergent from all primate sequences to be inserted in figure 3 , where only variable positions are given.
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Polymorphism of Intron 3 in Chimpanzees and Gorillas
As human RHD and RHCE genes differ in the length of intron 3, we searched for a similar polymorphism in chimpanzees and gorillas wuth a PCR assay (see Materials and Methods). From all chimpanzee DNA samples (N = 105), products of lengths equivalent to those of human RHCE and RHD, respectively, were obtained. RHD-like and RHCE-like intron 3 fragments were obtained with 7 of 15 gorilla DNA samples. With the remaining eight gorilla samples, only RHCE-like fragments were obtained (table 1
). Partial sequences of the chimpanzee and gorilla PCR products confirmed that they were amplified from RH-like genes (data not shown).
Types of Associations Between Introns 3 and Introns 4 and Estimated Frequencies of RH Haplotypes in Chimpanzees
In order to investigate the various combinations of introns 3 and 4 in chimpanzee and gorilla RH-like genes, amplifications of long genomic fragments were performed. Fragments were separated on the basis of their lengths and were checked by sequencing for the presence of RHD-like specific deletions in introns 3 and 4 and for the presence of the RHD-like type 2 addition in intron 4 (see Materials and Methods and fig. 1
). From one chimpanzee and one gorilla, DNA fragments of 2 kb containing RHCE-like introns 3 and 4 were amplified by means of a primer in intron 3 (RB46-dir) and a primer specific to the RHCE intron 4 (RHCE.Int.4-rev). The corresponding RH-like genes were named "Chimp CE/CE" and "Gor CE/CE" (fig. 6
). Partial sequencing of chimpanzee and gorilla amplicons confirmed the presence of RHCE-specific elements in intron 3 and intron 4.
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All attempts to amplify genomic fragments associating RHCE-like introns 3 with RHD-like type 1 introns 4 in chimpanzees and gorillas failed. Therefore, the existence of a gorilla RH-like gene associating RHCE-like introns 3 with type 1 RHD-like introns 4 is questionable. It is possible, therefore, that these two introns do not belong to the same gene (the h1 haplotypes of gorillas and chimpanzees in fig. 6 ).
As 105 randomly selected chimpanzees (table 1
) were studied for the presence of introns 3 and 4, one can propose the existence of at least three RH haplotypes and estimate their frequencies. The presence of three RH-like genes per chimpanzee haploid genome was previously demonstrated (Blancher, Calvas, and Ruffié 1992
); therefore, only three-gene haplotypes are proposed in this paragraph. Three chimpanzees possessed a phenotype suggesting that they were homozygotes for haplotype h1 (frequency = p) which associates RH-like genes [D/D1CE/??/?], defined by intron 3/4 combinations (question marks indicate the impossibility of assessing the type of intron). According to the Hardy-Weinberg binomial formula, the frequency of haplotype 1 is p2. In the same way, 85 animals are expected to be either homozygotes of haplotype h2 (frequency = q) [D/D1CE/D2?/?] or heterozygotes of haplotypes h1 and h2, and the calculated frequency is q2 + 2pq. Finally, 17 chimpanzees are considered either homozygotes of haplotype h3 (frequency = r) [D/D1?/D2CE/CE] or heterozygotes of haplotypes h2 and h3, with a frequency of r2 + 2pr + 2qr. The approximate estimations for p, q, and r are 0.17, 0.75, and 0.08, respectively, and are reported in figure 6
. Obviously, many other haplotype combinations can be proposed, and only the complete sequencing of the chimpanzee RH loci of numerous animals could lead to a definite description of chimpanzee haplotypes. In addition, the respective positions of RH-like genes in chimpanzee and gorilla genomes, as shown in figure 6
, are arbitrary.
The 15 gorillas studied here displayed four types of combination between the three types of intron 4 and the two types of intron 3 (table 1 ). One of these combinations consisted in the presence of RHCE-like intron 3 and RHD-like intron 4 of type 1 (six gorilla samples). From the existence of this combination, one can infer that in some gorilla genes, RHCE-like intron 3 is associated with type 1 RHD-like intron 4. However, the existence of such a gorilla RH-like gene is questionable, because, as mentioned above, all attempts to amplify genomic fragments associating RHCE-like introns 3 with type 1 RHD-like introns 4 in gorillas failed.
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Discussion |
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Some chimpanzee and gorilla RH-like genes were shown to possess RHCE-like introns 4 encompassing an Alu-Sx element in a position orthologous to that observed in the human RHCE gene. However, whereas all humans, apart from exceptional variants (Huang 1997a
), possess the RHCE gene, and thus introns 4 of the RHCE type, only 16% of the chimpanzees (17/105) and 33% of the gorillas (5/15) studied here possessed an RHCE-like intron 4.
Like humans, chimpanzees and gorillas also possess RHD type introns 4. Both human RHD and ape RHD-like introns 4 are deprived of a 654-bp segment which is present in RHCE and RHCE-like introns 4. In addition, some chimpanzee and gorilla RHD-like introns 4 exhibit an additional 12mer repetitive DNA segment 3' of the deleted region. Introns without the repeat were referred to as type 1 RHD-like, while RHD-like introns 4 with the 12mer segment were designated type 2 RHD-like. The chimpanzee type 2 RHD-like intron 4 is homologous to the chimpanzee sequence previously reported by Westhoff and Wylie (1996)
.
In humans, the absence of RHD is frequent (15% of Caucasians are D-negative and do not possess RHD introns 4 in their genomes). In African apes, absence of RHD-like introns 4, if possible, must be infrequent, as all chimpanzees and all gorillas tested so far have possessed type 2 and/or type 1 RHD-like introns 4. However, it has to be noted that in the series reported by Westhoff and Wylie (1996)
, one gorilla out of five and no chimpanzees out of seven were negative for the amplification of RHD-like introns 4.
The great homology between human RHD intron 4 and RHD-like (type 1 and type 2) introns 4 of gorillas and chimpanzees suggests a common ancestry of the corresponding genes. One can hypothesize that the common ancestor gene of RHD and RHD-like genes already displayed the deletion encompassing the Alu-Sx, together with 354 bp 5' of the Alu. Although excision of Alu elements is a rare event, examples have previously been described by others (Meagher, Jorgensen, and Deeb 1996
; Satta, Mayer, and Klein 1996
). It is noteworthy that only chimpanzees and gorillas possess type 2 RHD-like intron 4, which differs from type 1 RHD-like intron 4 by the presence of a 12mer repeat (fig. 3
). Indeed, using a pair of primers adapted to the detection of RHD-like intron 4 polymorphisms (presence or absence of the 12mer repeat), we demonstrated by PCR the absence of a counterpart of the apes type 2 RHD intron 4 in 72 human genomic samples. Taken together, the results suggest that the common ancestor of chimpanzees and gorillas, which is supposed to also be the ancestor of humans, possessed the type 2 RHD intron 4. This led us to conclude that humans, unlike chimpanzees and gorillas, most probably lost the gene(s) harboring type 2 RHD intron 4. To verify this hypothesis, it remains to identify the relicts of this deletion in the human genome and to study a larger number of individuals, because the RHD type 2 intron could be present in humans at very low frequency and perhaps only in some peculiar populations.
As reported elsewhere, gorillas express a D-like polymorphic antigen called Dgor (Roubinet et al. 1993
). The expression of the Dgor antigen is associated with restriction length polymorphisms revealed by probing Southern blots with an exon-4-specific probe and with a PCR length polymorphism of the RHD-like intron 3 (Apoil, Roubinet, and Blancher 1999
). In the present study, we demonstrate that the expression of Dgor is also associated with the presence in the gorilla genome of the type 2 RHD-like intron 4 and, therefore, with the presence of a haplotype associating the "Gor D/D1" and "Gor CE/D2" genes (fig. 6
and table 1
). However, it is not possible to ascribe one of these two genes to the expression of Dgor. Gorilla RH-like genes associated with type 1 RHD intron 4 are also functional because Dgor-negative animals are agglutinated by some anti-D reagents and express Rh-like proteins at the surfaces of their red blood cells (RBCs) (Blancher and Socha 1997
; Apoil, Roubinet, and Blancher 1999
). Although it is not possible to assess whether or not the gorilla Gor CE/CE gene is functional, partial exon sequences of this gene established its homology with the human RhcE cDNA (Apoil, Roubinet, and Blancher 1999
). In chimpanzees, the absence of association between the intron 3 and 4 PCR patterns and the R-C-E-F types or the restriction patterns (data not shown) did not allow us to specify the functionality of the RH-like genes described here. However, all of the animals studied here were agglutinated by some anti-D monoclonal reagents and express Rh-like proteins at the surfaces of their RBCs.
Chimpanzees and gorillas displayed a great variety of combinations between the various types of RH-like introns 3 and 4. This variety is indirect evidence that intergenic exchanges between RH-like genes in these two species were not infrequent (Blancher and Socha 1997
). However, if these intergenic exchanges were frequent, they would have homogenized RH sequences in each species. This would result in a clustering of sequences by species. In fact, this is not observed in the phylogenetic tree presented in figure 5 .
In conclusion, the numerical chromosomal polymorphism of RH genes (the human locus displays either one or two genes, the chimpanzee possesses three, and the gorilla possesses two) suggests that unequal crossing over most probably arose after the original duplication of the RH ancestor gene. Intergenic exchanges by various mechanisms (double crossing over, homologous recombination, or gene conversion) between RH genes have occurred in humans and the two other species possessing more than one RH gene (i.e., chimpanzees and gorillas). However, despite these intergenic exchanges, the coding regions of human RHD and RHCE genes (417 codons) differ by 41 nucleotide substitutions, with 35 being nonsynonymous. This suggests that the differentiation of the two human RH genes (RHD and RHCE) was maintained because it represented a selective advantage.
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Acknowledgements |
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Footnotes |
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1 Keywords: RH genes,
evolution,
intronic polymorphism,
Alu elements.
2 Address for correspondence and reprints: Antoine Blancher, Laboratoire dImmunologie, Centre Hospitalier Universitaire Purpan, 31059 Toulouse cedex, France. E-mail: blancher{at}mail.easynet.fr
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