Division of Environmental Microbiology, Institute for Animal Health, Compton Laboratory, Compton, Newbury RG20 7NN, UK1
Author for correspondence: Mark P. Stevens. Tel: +44 1635 578411. Fax: +44 1635 577243. e-mail: mark-p.stevens{at}bbsrc.ac.uk
Keywords: Escherichia coli, Shiga toxin, colonization, bovine, infection
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Overview |
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Epidemiology of EHEC |
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The O-serotype predominantly associated with human infections in Great Britain is O157 and isolations have almost doubled in the last 6 years (Anon, 2000 ). Other EHEC serotypes (particularly O26, O111, O128 and O103) are emerging as a common cause of human disease in many countries and indeed may be more prominent than O157 (Bettelheim, 2000
). It is presently unclear if the incidence of human infections caused by these serotypes correlates with the prevalence of the bacteria in farm animal hosts. In a recent survey of non-O157 Shiga-toxin-producing E. coli isolates from Scottish cattle, 8 of the 11 different O-serogroups represented have also been associated with bloody diarrhoea in humans and HUS (Jenkins et al., 2002
). Interestingly, O26 and O111 EHEC were not isolated in this study, although they have been isolated from healthy cattle in other studies (Kobayashi et al., 2001
). It remains unclear if all EHEC present in animal reservoirs present a risk to the human population. Further, it is not known why some EHEC serotypes (e.g. O157) can cause disease in humans but not in cattle. EHEC isolated from healthy cattle often possess the same combinations of putative virulence genes that are found in human isolates (Wieler et al., 1996
; Sandhu et al., 1996
; Kobayashi et al., 2001
; Jenkins et al., 2002
). However, differences in expression of these genes have been detected among human and bovine isolates in vitro and this may affect the potential for zoonotic transmission (McNally et al., 2001
).
In a recent 12-month survey of cattle and sheep presented for slaughter at abattoirs in Great Britain, 4·7% of cattle and 1·7% of sheep contained E. coli O157 in the faeces (Paiba et al., 2002 ). Further, it was reported that up to 44% of cattle herds in Great Britain have at least one animal shedding E. coli O157 (Paiba et al., 2002
). The E. coli O157 phage types most frequently isolated from cattle (2, 8 and 21/28) and sheep (4 and 32) were the same five types predominantly associated with illness in humans in Great Britain during the same period (Paiba et al., 2002
). The incidence of EHEC serotypes other than O157 that may pose a risk to humans was not determined in this study. In the United States, the prevalence data are even higher, with an estimated 28% of beef cattle shedding E. coli O157:H7 in the summer months (Gansheroff & OBrien, 2000
; Elder et al., 2000
).
The prevalence of EHEC is higher in younger animals and in animals subject to transit, feed changes and antimicrobial therapy, possibly due to disturbance in the resident intestinal microflora (Hancock et al., 1998 ). The shedding patterns are characterized by sharp bursts of EHEC excretion in a high percentage of animals separated by longer periods of very low prevalence. E. coli O157:H7 is capable of efficient transmission between animals, even at very low doses (Besser et al., 2001
). To add to the problem, animals colonized by EHEC are hard to identify since E. coli O157:H7 infections in weaned calves and adult cattle and sheep are asymptomatic (Cray & Moon, 1995
; Brown et al., 1997
; Dean-Nystrom et al., 1998a
, 1999
; Cornick et al., 2000
; Wray et al., 2000
). The infectious dose for E. coli O157:H7 in humans has been estimated to be less than 100 c.f.u. (Griffin, 1995
). Calves and adult cattle experimentally infected with EHEC can excrete large numbers of EHEC for up to several weeks depending on the inoculation dose (Cray & Moon, 1995
; Brown et al., 1997
; Sanderson et al., 1999
; Cornick et al., 2000
; Wray et al., 2000
). The magnitude and duration of excretion of EHEC by naturally infected animals is less well studied, therefore the risk to human health posed by ruminants colonized by EHEC is difficult to quantify. Some EHEC serotypes (e.g. O5, O26 and O118) are associated with enteric disease in farm animals and cause significant morbidity and mortality (Mainil, 1999
; Bettelheim, 2000
).
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Host responses to EHEC infection |
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Natural and experimental infection of susceptible calves with bovine virulent EHEC serotypes frequently produces diarrhoea, villous atrophy, epithelial cell damage, infiltration of neutrophils into the lamina propria and intestinal lumen and the formation of a pseudomembrane containing blood, fibrin, cellular debris and neutrophils (Hall et al., 1985 ; Chanter et al., 1986
; Pearson et al., 1999
; Mainil, 1999
). Similar histopathological lesions occur in some cases of EHEC-associated haemorrhagic colitis in humans (Slutsker et al., 1997
).
Distal sites in the intestine such as the caecum, colon and rectum are believed to be the principal sites of E. coli O157:H7 colonization in ruminants (Cray & Moon, 1995 ; Brown et al., 1997
; Dean-Nystrom et al., 1998a
, 1999
; Wales et al., 2001a
, b
; Grauke et al., 2002
). We and others have observed little, if any, direct interaction between E. coli O157:H7 and the epithelium of the lower gastrointestinal tract in experimentally infected conventional calves (M. Stevens, unpublished observations; Wray et al., 2000
). In contrast, extensive bacterial adherence to the colonic epithelium is seen in calves infected with EHEC serotype O5 (Hall et al., 1985
; Chanter et al., 1986
), O26 (Pearson et al., 1999
) and O111 (Stevens et al., 2002b
). Similar observations have been made in bovine ileal and colonic loops infected with O157 and non-O157 strains (Sandhu & Gyles, 2002
). Thus E. coli O157 and non-O157 serotypes may use distinct mechanisms to colonize the ruminant host. Differences in tissue tropism may have implications for the control of EHEC colonization in cattle and raise important questions about the likely efficacy of mucosal immune responses against bacteria existing in different intestinal niches.
Serum antibody responses against the O157 lipopolysaccharide and Shiga toxin 1 have been detected in some but not all experimentally infected calves (Johnson et al., 1996 ; Wray et al., 2000
) and sheep (Cornick et al., 2000
). Neutralizing antibody responses against Stx2 were not detected following E. coli O157:H7 infection of calves (Johnson et al., 1996
). Anti-LPS and Stx1 responses in calves could not be correlated with the elimination of E. coli O157:H7 and failed to reduce faecal shedding of the bacteria following re-inoculation with the same strain (Johnson et al., 1996
; Wray et al., 2000
). In contrast, Sanderson et al. (1999)
reported that calves re-infected with E. coli O157:H7 shed the organism for a shorter duration following the second or third inoculation. Diarrhoeic calves experimentally infected with an O5 calf dysentery EHEC strain did not present with dysentery following a second challenge (Chanter et al., 1986
). However, susceptibility of calves to this strain is age-related (Chanter et al., 1986
), therefore the lack of symptoms following re-inoculation may not necessarily be the result of acquired immunity. Further studies are required to measure the induction and efficacy of humoral and cell-mediated immune responses to EHEC following natural and experimental infection of ruminants.
The induction of intestinal secretory and inflammatory responses to different EHEC serotypes and defined bacterial mutants has been quantified in bovine ligated intestinal loop models (Stevens et al., 2002a ; Sandhu & Gyles, 2002
). Whilst O157 strains were not strongly enteropathogenic, fluid accumulation and a pronounced infiltration of neutrophils was seen in response to EHEC serotypes O103 (Stevens et al., 2002a
) and O5, O26 and O111 (Sandhu & Gyles, 2002
). This provides a valuable model to dissect the role of bacterial factors in EHEC-induced enteritis and intestinal colonization.
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Factors mediating colonization of the bovine intestine by EHEC |
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Intimin has been shown to play a key role in intestinal colonization in colostrum-deprived neonatal calves and piglets by E. coli O157:H7 and in the induction of colonic oedema and diarrhoea (Donnenberg et al., 1993 ; McKee et al., 1995
; Tzipori et al., 1995
; Dean-Nystrom et al., 1998b
). Intimin also influences colonization of adult cattle and sheep (Cornick et al., 2002
). However, the eae gene is not present in all Shiga-toxin-producing E. coli strains isolated from diarrhoeic calves and healthy cattle (Wieler et al., 1996
; Sandhu et al., 1996
; Jenkins et al., 2002
), indicating that other factors influencing persistence of the bacteria in the bovine intestine may be important. The finding that eae null mutants still colonize some compartments of the intestinal tract of calves and sheep (Dean-Nystrom et al., 1998b
; Cornick et al., 2002
) supports the notion that accessory adhesins and/or growth in the lumenal contents may influence carriage. A number of loci have been implicated in the attachment of EHEC to epithelial cells in vitro, including LEE-encoded EspA filaments (Ebel et al., 1998
), Iha (Tarr et al., 2000
) and several other genes identified by transposon mutagenesis of E. coli O157:H7 (Tatsuno et al., 2000
). To our knowledge, the role of these genes in colonization of the ruminant intestine has not yet been tested.
LEE-negative Shiga-toxin-producing E. coli strains have also been implicated in outbreaks of human disease and HUS (Paton et al., 1999 ). Recently, two factors encoded on the large plasmid of an O113:H21 LEE-negative isolate implicated in an outbreak of HUS were found to mediate attachment of the bacteria to cultured epithelial cells. One is an autoagglutinating adhesin (Saa) (Paton et al., 2001
) and the other is a novel type IV pilus (Srimanote et al., 2002
). The distribution of the saa and pil loci among LEE-negative EHEC strains isolated from ruminants and their role in intestinal colonization remains to be studied.
Recently, we have identified a gene (efa1) that mediates colonization of the bovine intestine by non-O157 EHEC (Stevens et al., 2002b ). Efa1 (EHEC factor for adherence) was recently implicated in adherence of a clinical O111:H- EHEC strain to cultured Chinese Hamster Ovary cells (Nicholls et al., 2000
). Mutation of the efa1 gene of EHEC serotypes O5 and O111 significantly reduced faecal shedding and bacterial adherence to the colonic epithelium in experimentally infected calves (Stevens et al., 2002b
). EHEC strains harbouring the efa1 mutation were found to express and secrete slightly less of the LEE-encoded effector proteins (Stevens et al., 2002b
).
The efa1 gene is present in all non-O157 strains so far tested (Nicholls et al., 2000 ; Klapproth et al., 2000
), and is identical in size and 99·9% identical in nucleotide sequence to a gene in EPEC (lifA) encoding a lymphocyte inhibitory factor (lymphostatin). Lymphostatin confers the ability to inhibit the proliferation of human peripheral blood lymphocytes and the mitogen-stimulated synthesis of interleukin-2, -4, -5, and interferon-
(Klapproth et al., 2000
). The predicted product of lifA also inhibits the proliferation of human and murine gastrointestinal lymphocytes, indicating that it may modulate mucosal immunity in the gut (Malstrom & James, 1995
; Klapproth et al., 1996
). EHEC strains can inhibit the proliferation of bovine peripheral blood monocytes, however it is not yet clear if this activity is attributed to the efa1 gene (Stevens et al., 2002b
). Thus Efa1 may influence colonization of the bovine intestine in several ways. It may act as an adhesin per se, it may promote EHEC survival in the bovine gut by modulating mucosal immunity, or it may act indirectly by influencing the expression and secretion of LEE-encoded proteins or other membrane-associated proteins that influence colonization.
Whilst E. coli O157:H7 lacks the full-length efa1 gene, analysis of the genome sequence has revealed that a truncated version of the efa1 gene encoding the N-terminal 433 amino acids of the protein exists in the chromosome (Stevens et al., 2002b ). A transposon insertion upstream of the truncated efa1 gene was recently shown to reduce bacterial adherence to human colon carcinoma cells (Tatsuno et al., 2000
). E. coli O157:H7 strains also encode a homologue of efa1 (toxB/l7095) on the pO157 virulence plasmid (Nicholls et al., 2000
). E. coli O157:H7 strains containing derivatives of pO157 that lack toxB exhibit reduced adherence to cultured epithelial cells, possibly owing to a reduction in the production and secretion of LEE-encoded type III secreted proteins (Tatsuno et al., 2001
). The toxB gene has also been suggested to be a functional homologue of lymphostatin, since an E. coli O157:H7 strain cured of pO157 lacked the ability to inhibit IL-2 and IL-4 synthesis in mitogen-activated human PBMCs (Klapproth et al., 2000
). Thus the E. coli O157:H7 truncated efa1 and toxB genes may influence intestinal colonization in ruminants in a similar manner to efa1 in non-O157 EHEC.
The role of Shiga toxins in EHEC intestinal colonization and enteropathogenesis in ruminants is unclear. Nearly all E. coli O157 strains isolated from animals at slaughter in Great Britain contain the stx2 gene (97·8% of cattle isolates and 100% of sheep isolates), and 19·9 and 1·4%, respectively, contained both stx1 and stx2 genes (Paiba et al., 2002 ). This suggests a strong correlation between possession of stx genes and colonization of the ruminant host. Receptors for Stx have been detected on bovine intestinal crypt cells (Hoey et al., 2002
), and it has been reported that purified Stx1 can inhibit the activation and proliferation of bovine lymphocytes (Menge et al., 1999
; Ferens & Hovde, 2000
). Thus Stx may facilitate intestinal colonization by modulating the mucosal immune response. Dean-Nystrom et al. (1998a
) reported that an Stx-producing E. coli O157:H7 strain colonized weaned calves better than a non-producing strain; however, the strains used were not isogenic and differences in colonization may have been due to traits other than Stx production. Stx1 does not appear to be involved in the induction of intestinal secretory and inflammatory responses to EHEC serotype O103:H2 in bovine ligated ileal loops (Stevens et al., 2002a
).
The role of flagella, fimbriae and other surface appendages in colonization of the ruminant intestine by EHEC remains to be tested. Flagella and/or motility are important for colonization, invasion and persistence in several enteric pathogens (La Ragione et al., 2000 ; Allen-Vercoe et al., 1999
). The EPEC H6 flagellin was recently shown to mediate adherence to epithelial cells (Girón et al., 2002
); however, H7 flagella expressed by many E. coli O157 isolates lacked this activity. E. coli O157:H7 encodes 14 different fimbrial operons, however it is presently unclear which if any of these contribute to adherence. Indeed, E. coli O157 contains intact genes for the expression of type I fimbriae, however these are not expressed owing to a deletion in the fimA promoter (Roe et al., 2001
).
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Insights from comparative and functional genomics in E. coli O157:H7 |
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Given the size of the E. coli O157:H7 genome (approx. 5·5 Mb) and the large number of O157-specific genes, we have adopted a random mutagenesis strategy to identify EHEC genes required for colonization and survival in cattle using signature-tagged transposon mutagenesis (STM). STM makes it possible to track the fate of individually tagged bacterial transposon mutants in a complex pool during their interaction with the animal host (Hensel et al., 1995 ). The composition of a pool of random mutants in the inoculum can be compared with the composition of a pool of mutants recovered from the faeces at time-points after oral inoculation of calves. This involves the use of PCR and DNA hybridization to detect oligonucleotide sequence tags unique to each mutant in the pool (Hensel et al., 1995
). In this way it is possible to identify EHEC transposon mutants deficient in intestinal colonization and/or survival in the bovine host. Factors required for colonization can then be identified by cloning and sequencing of the disrupted genes. Recently we have shown that mini-Tn5Km2 insertions in genes encoding structural components of the LEE-encoded type III protein secretion system (escC, escV and escN) result in reduced faecal excretion of E. coli O157:H7 (P. van Diemen & F. Dziva, unpublished observations). This finding indicates that LEE-mediated adherence of E. coli O157:H7 to intestinal epithelia and AE-lesion formation is important for colonization and suggests that the bacteria are not merely transient members of the lumenal microflora requiring continuous re-inoculation. The site(s) of specific adherence of E. coli O157:H7 to the intestinal epithelium in calves remain to be determined. LEE-encoded genes are also crucial for colonization of the rabbit intestine by rabbit EPEC (Abe et al., 1998
; Marchés et al., 2000
). Given that EHEC O157 and non-O157 serotypes potentially use different strategies to colonize the ruminant host we are also employing STM in an O26 calf dysentery isolate with recovery of bacterial mutants direct from the colonic mucosa. This may shed light on why some EHEC serotypes extensively interact with the intestinal epithelium whereas E. coli O157 apparently exists largely in the intestinal lumen. The information derived from screening tagged EHEC O157 and O26 transposon mutants in cattle will greatly facilitate the development of strategies to reduce the prevalence of EHEC in farm animal hosts. E. coli O157:H7 has been reported to undergo rapid genetic diversification following oral inoculation of calves with a single clonal isolate (Akiba et al., 2000
). It is presently unclear what effect this will have on the long-term efficacy of strategies to control EHEC.
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Control by vaccination |
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Immune responses against Int280 peptides are subtype-specific and do not protect against challenge with C. rodentium strains expressing heterologous intimin subtypes in the murine model (Ghaem-Maghami et al., 2001 ). Immunization of mice with a conserved domain of intimin (Int388667) was not effective. Thus intimin-based vaccines may have to incorporate several different Int280 subtypes in order to protect against the predominant EHEC serotypes in cattle (ß,
and
). It is possible that vaccines based on the Efa1 colonization factor may avoid complications of subtype-specificity. The Efa1 protein is expressed at the bacterial cell surface (Nicholls et al., 2000
; M. Stevens, unpublished observations) and is present in all non-O157 EHEC strains tested to date (Nicholls et al., 2000
; Klapproth et al., 2000
). E. coli O157:H7 strains encode a truncated version of the efa1 gene encoding the N-terminal domain of the protein. Thus subunit vaccines based on the N-terminus of Efa1 have the potential to elicit broadly reactive immunity against all EHEC serotypes.
Conjugate and toxoid vaccines based on the O157 lipopolysaccharide elicit specific antibody responses in mice and human volunteers (Konadu et al., 1998 , 1999
; Conlan et al., 1999a
, 2000
). However, oral and parenteral immunization of mice with an O157-glycoconjugate vaccine failed to protect against intestinal colonization with E. coli O157:H7 (Conlan et al., 1999a
, 2000
). Calves make O-antigen-specific antibodies in response to E. coli O157 infection (Johnson et al., 1996
; Wray et al., 2000
); however, the induction of the antibody response could not be correlated with clearance of the bacteria.
Butterton et al. (1997) investigated the feasibility of using a live attenuated Vibrio cholerae El Tor vaccine strain (Peru2) expressing the E. coli O157:H7 intimin gene for protection against EHEC in a rabbit model. A plasmid construct for the expression of eae in Peru2 proved to be unstable and failed to elicit intimin-specific antibody responses following oro-gastric inoculation of rabbits (Butterton et al., 1997
). However, a Peru2 derivative with a chromosomal insertion of the eae gene induced a marked anti-Eae response in one of two rabbits. With development it should be possible to improve the expression of heterologous antigens in an appropriate bacterial carrier for cattle.
Previous studies have shown that oral vaccination of calves with a Salmonella typhimurium aro vaccine strain expressing fragment C of tetanus toxoid (TetC) induces potent mucosal IgA and serum IgG responses against TetC (Villareal-Ramos et al., 2000 ). Thus delivery of EHEC antigens by live attenuated Salmonella strains is a viable option to induce relevant EHEC-specific mucosal immunity. Salmonella strains expressing lipopolysaccharide O-antigens of the same serogroup as prevalent EHEC isolates (O157 and O111) have been developed and offer some protection in murine models (Conlan et al., 1999b
; Wang et al., 1999
). Salmonella carrier vaccines have the added advantage of inducing solid protection against virulent Salmonella strains in calves (Jones et al., 1991
). Live attenuated vaccines offer the advantage of in vivo amplification of the antigen, prolonged exposure of the mucosal immune system to the antigen and contact immunization via excreted organisms. However, such vaccines may be inhibited by competing organisms in the intestinal microflora and the presence of antibiotics or antibodies in the feed or milk. Further, live bacterial vaccines are unlikely to be used in food-producing animals until fears over safety and the potential for zoonotic transmission to humans are addressed. Kaper et al. (1998)
suggested that edible vaccines based on transgenic plants engineered to express intimin may offer another mode of delivery. Transgenic plant vaccines are capable of inducing substantial mucosal immune responses in murine models (Haq et al., 1995
).
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Passive protection |
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Probiotics and other inhibitors |
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Effect of diet and fasting |
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Reports on the effect of diet and diet shifts on shedding of E. coli O157:H7 in ruminants are often conflicting. Grain feeding has been proposed as a risk factor for increased shedding of EHEC in cattle (Diez-Gonzalez et al., 1998 ; Dargatz et al., 1997
). However, Hancock et al. (1994)
and Magnuson et al. (2000)
found no difference in the amount or duration of E. coli O157:H7 shedding between animals fed grain and forage diets. Indeed, grain-fed sheep shed E. coli O157:H7 for a significantly shorter duration under some conditions than sheep fed hay (Kudva et al., 1997
). Experimentally inoculated calves fed on a primarily grain-based diet develop a lower rumen pH and shed elevated numbers of acid-tolerant E. coli O157:H7 than calves fed on a high-roughage (hay-based) diet (Diez-Gonzalez et al., 1998
; Tkalcic et al., 2000
). Acid-tolerant EHEC may be expected to colonize other animals, and possibly humans, more efficiently since they may survive better in the acidic compartments of the intestine. Thus grain diets may actually facilitate transmission of the bacteria in the farm environment. A brief period of feeding hay to grain-fed calves has been reported to decrease the number of acid-resistant E. coli O157:H7 shed substantially (Diez-Gonzalez et al., 1998
) and may help to reduce entry of acid-resistant EHEC into the food chain. However, Hovde et al. (1999)
found that hay-fed animals shed E. coli O157:H7 for longer than grain-fed animals, and that irrespective of diet, equal numbers of acid-resistant EHEC were shed. Further studies are required to determine the influence of different diets on the shedding and infectivity of EHEC in ruminants.
Other foodstuffs including cottonseed, clover or lucerne hay and soymeal have been reported to reduce the shedding of E. coli in cattle (Brownlie & Grau, 1967 ; Dargatz et al., 1997
; Garber et al., 1995
), while barley (Dargatz et al., 1997
), corn silage (Herriott et al., 1998
) and beetpulp (Schouten et al., 2001
) appear to increase carriage rates. The relative abundance in plants of secondary metabolites inhibitory to EHEC may play a role in this (Duncan et al., 2000
).
Fasting is common before and during transportation of animals to abattoirs and is intended to reduce soiling of the hide. This practice may inadvertently increase the proliferation of EHEC in the intestine by causing the rumen pH to rise and the concentration of volatile fatty acids to drop (Duncan et al., 2000 ). Cray et al. (1998)
reported that withholding food for 48 h prior to inoculation rendered calves more susceptible to E. coli O157:H7 infection and that significantly more bacteria were shed compared with animals fed without interruption. Similar observations have been made in rams deprived of feed and water (Kudva et al., 1995
). Withdrawal of food from calves carrying EHEC has been shown to increase faecal shedding in some previously low-shedding calves (Brown et al., 1997
), and was variable in calves shedding larger populations (Brown et al., 1997
). However, Cray et al. (1998)
found no effect on shedding levels of withdrawing feed after E. coli O157:H7 infection of calves. An important consideration when comparing the results of such studies may be the accuracy of quantification of EHEC excretion rates and the possible influence of water content on the count of bacteria per unit weight of sample.
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Impact of farm management practices |
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Perspective |
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ACKNOWLEDGEMENTS |
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