1 Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA
2 United States Department of Agriculture, Agricultural Research Service, Horticultural Crops Research Laboratory, 3420 NW Orchard Avenue, Corvallis, OR 97330, USA
Correspondence
Joyce Loper
loperj{at}science.oregonstate.edu
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ABSTRACT |
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INTRODUCTION |
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In Escherichia coli, the sigma factor S (or
38), encoded by rpoS, is a central regulator of gene expression by cells in stationary phase (Hengge-Aronis, 2002a
, b
; Kolter et al., 1993
). Under conditions of cellular starvation,
S accumulates, binds and directs the RNA polymerase holoenzyme to over 100 genes constituting the RpoS regulon (Ishihama, 2000
). Members of the regulon encode proteins with diverse functions, including stress response. Consequently, a mutation in rpoS has pleiotropic effects on cells in stationary phase, including diminished survival when subjected to environmental stresses (Hengge-Aronis, 2002a
, b
; Kolter et al., 1993
). Many of the temporal changes in cell morphology and physiology that occur during the transition from exponential growth to stationary phase are similar in E. coli and Pseudomonas spp. For example, members of both genera exhibit reductions in cell size, DNA and ribosome content, and the rate of protein synthesis upon entry to stationary phase (Venturi, 2003
). In Pseudomonas spp., as in E. coli,
S is required for optimal survival of stationary-phase cells exposed to various environmental stresses (Heeb et al., 2005
; Jørgensen et al., 1999
; Miller et al., 2001a
, b
; Miura et al., 1998
; Ramos-González & Molin, 1998
; Sarniguet et al., 1995
; Suh et al., 1999
; Whistler et al., 2000
). Nevertheless, recent studies imply that fundamental differences in the molecular events characterizing the transition between exponential growth and stationary phase may exist between the two genera (Venturi, 2003
).
The rhizosphere inhabitant Pseudomonas fluorescens strain Pf-5 is a biological control organism that suppresses plant diseases caused by soil-borne fungi and oomycetes (Howell & Stipanovic, 1979). Pf-5 produces an array of secondary metabolites, including the antibiotics pyoluteorin (Howell & Stipanovic, 1980
), 2,4-diacetylphloroglucinol (Nowak-Thompson et al., 1994
) and pyrrolnitrin (Howell & Stipanovic, 1979
), that are toxic to soilborne plant pathogens. Previous studies have demonstrated that an rpoS mutant of Pf-5 is altered in secondary metabolite production, overproducing pyoluteorin and 2,4-diacetylphloroglucinol, and lacking production of pyrrolnitrin (Pfender et al., 1993
; Sarniguet et al., 1995
). In addition to producing antibiotics, effective biological control organisms must colonize plant surfaces, where they encounter a myriad of environmental stresses. RpoS clearly has an important role in stress response of the biological control agent Pf-5, as an rpoS mutant is more sensitive than the parental strain to osmotic and oxidative stresses (Sarniguet et al., 1995
). Therefore, it is not surprising that rpoS is required for optimal survival of Pf-5 on surfaces of seeds (Sarniguet et al., 1995
) and wheat straw maintained under controlled experimental conditions (Pfender et al., 1993
).
The current study was initiated to expand our understanding of the role of rpoS in the stress response of P. fluorescens Pf-5. We demonstrated that rpoS has a role in the survival of Pf-5 exposed to starvation, freezing, desiccation and UV irradiation, all of which are likely to be encountered by bacteria inhabiting plant surfaces. The role of rpoS in establishment of Pf-5 on seed surfaces was demonstrated previously in experiments done in an environmentally controlled growth chamber (Sarniguet et al., 1995), but results from such experiments are not always predictive of those done in a field environment. Bacteria inhabiting plant surfaces in the field are influenced by many factors that are not reproduced in growth chambers, including differences in the physiology and growth of plants, environmental constraints imposed by variable weather patterns, and the presence of indigenous organisms. Therefore, in this study, we demonstrate that rpoS also has a role in the establishment and survival of Pf-5 in the rhizosphere under field conditions.
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METHODS |
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In field experiments, a spontaneous mutant of Pf-5 resistant to rifampicin (Pf-5R, JL3871) was used to facilitate estimates of rhizosphere population size. Pf-5R is similar to the parental strain in growth rate and production of secondary metabolites (data not shown). An rpoS mutant of Pf-5R (Pf-5R rpoS, JL4739) was generated for this study by marker-exchange mutagenesis. Briefly, an EcoRI genomic fragment containing rpoS : : Tn5 of JL3985 was cloned into pBR322 to generate pJEL1995 (Pfender et al., 1993). Marker-exchange mutagenesis of Pf-5R with DNA of pJEL1995 was performed as described previously (Whistler et al., 2000
). Southern analysis was used to determine that the resultant rifampicin-resistant, kanamycin-resistant strain (Pf-5R rpoS, JL4719) contains a single genomic Tn5 insertion, which is in rpoS. Like the rpoS mutant of Pf-5 (Sarniguet et al., 1995
), Pf-5R rpoS was found to be prototrophic, lacking detectable tryptophan-sidechain oxidase activity, lacking pyrrolnitrin production, and overproducing pyoluteorin and 2,4-diacetylphloroglucinol (data not shown). The response of Pf-5R rpoS to oxidative and freezing stress was evaluated prior to the field experiments, and its stress response was found to be similar to that of the rpoS mutant of Pf-5 (data not shown). Bacterial strains were maintained in nutrient broth containing 15 % (v/v) glycerol at 80 °C.
Culture conditions.
Pf-5 and derivatives were grown in LuriaBertani broth (LB) at 27 °C on a rotary shaker (200 r.p.m.). Exponential-phase cells were harvested from cultures after 6 h incubation, whereas stationary-phase cells were collected from cultures 4 h after the OD600 of cultures stopped increasing (i.e. they entered stationary phase), as described previously (Sarniguet et al., 1995). Bacterial cells were collected by centrifugation (5000 g, 2 min), washed in 10 mM phosphate buffer (pH 7·0), and suspended to a final density of
1x108 c.f.u. ml1 in buffer, unless stated otherwise.
For field experiments, bacterial strains were cultured for 3 days at 20 °C on Pseudomonas agar F (PAF; Difco) containing 50 µg rifampicin ml1, or 50 µg rifampicin ml1 and 50 µg kanamycin ml1, for culture of Pf-5R or Pf-5R rpoS, respectively. Cells were scraped from the surface of the agar, and suspended in phosphate buffer. Each suspension was adjusted to 1x1010 c.f.u. ml1 prior to treatment of plant materials.
Stress tolerance assays.
Bacterial cells were cultured in broth as described above. After exposure to stresses imposed in the laboratory, dilutions of cells were spread on PAF, or PAF with 50 µg kanamycin ml1, for enumeration of Pf-5 or Pf-5 rpoS, respectively. Each assay contained at least three replicates of each strain, and all experiments were repeated at least three times.
Tolerance to UV irradiation was measured by methods described by Whistler et al. (2000). Briefly, dilutions of cell suspensions were spread onto PAF, and immediately exposed to UV irradiation (
254 nm) at doses up to 160 J m2. After UV irradiation, plates were incubated in darkness for 3 days; afterwards, colonies were counted.
Survival of starvation stress was assessed by incubating stationary-phase cells in 1 mM sterile potassium phosphate buffer (pH 7) containing 0·8 % (w/v) NaCl, with agitation (200 r.p.m.) at 27 °C for up to 36 days.
Two methods were used to determine the sensitivity of Pf-5 and derivatives to desiccation stress. In the first method (Stockwell et al., 1998), 10 µl samples of bacterial cells suspended in sterile distilled water (108 c.f.u. ml1) were placed on 1 cm2 sterilized Whatman no. 1 filter paper. The filters were suspended over a saturated solution of NaCl in sealed containers held at 25 °C to maintain a relative humidity of 75 % (Lindow et al., 1993
). Control sets of filters were suspended in chambers containing water and 100 % relative humidity. Periodically, filters were removed and placed in tubes containing 10 mM phosphate buffer (pH 7·0), and tubes were placed in an ultrasonic bath for 3 min prior to spreading dilutions on PAF. In a second desiccation stress assay, 10 µl samples of bacterial suspensions in water or 100 mM trehalose were spotted on autoclaved 1 cm2 glass squares, and placed in a sterile transfer hood, with the blower generating an air flow of 2 km h1. Glass squares were removed periodically, and culturable bacterial populations were enumerated as described for the desiccation assay with filter paper.
Tolerance of mild heat shock was measured by exposure of 100 µl bacterial suspensions to 42 °C for 5 or 20 min. After heating, 900 µl phosphate buffer (20 °C) was added, and the suspension was mixed using a vortex for 30 s prior to dilution plating.
To determine the relative tolerance of Pf-5 and derivatives to freezing, 100 µl samples of bacterial suspensions in water or 100 mM trehalose (Sigma) were incubated at 20 or 80 °C. After 1218 h, 900 µl phosphate buffer (20 °C) was added to rapidly thaw the frozen suspension. Samples were mixed using a vortex for 30 s, prior to dilution plating.
Colonization of bean seeds and emerging roots in growth chambers.
Bean (Phaseolus vulgaris cv. Bush Blue Lake 274) seeds were surface disinfected by soaking in 70 % ethanol for 1 min, and a 1 % hypochlorite solution for 15 min, followed by three rinses in sterile distilled water. Seeds were placed on sterile damp filter paper for 36 h at room temperature. Pre-germinated seeds were soaked in 2x108 c.f.u. ml1 suspensions of Pf-5 or Pf-5 rpoS for 10 min, prior to planting in individual pots containing 20 g pasteurized soil (Newberg fine sandy loam that passed through a 2 mm screen). Water was added to approximate a soil matric potential of 0·02 MPa (moist soil) or 1·0 MPa (dry soil), as determined from a soil moisture release curve. Pots were arranged in a randomized complete block, and maintained in a growth chamber at 20 °C without light. Ten seeds or seedlings for each soil moisture and bacterial treatment were harvested 1, 2, 3 and 5 days after planting. Plant material and adhering soil were placed in 5 ml 10 mM phosphate buffer, and subjected to vibrations in an ultrasonic cleaner for 5 min. Culturable populations of bacteria from the surface of bean seedlings were estimated by spreading dilutions of seed or seedling washes on PAF for recovery of Pf-5, or PAF containing 50 µg kanamycin ml1 for enumeration of Pf-5 rpoS.
Survival in the rhizosphere in field plots.
Field plots were established at the Oregon State University Botany and Plant Pathology Experimental Farm in Corvallis on 6 July 1997, 11 September 1997, and 26 July 1998. The soils in this area are characterized as Newberg fine sandy loam. Plots were split according to water application. Dry soil blocks were hoed daily for 3 days prior to planting. Moist soil blocks also were hoed, but water was added to saturation daily for 3 days prior to planting. Soil moisture content was estimated at each sample time by gravimetric methods. The soil moisture content of dry soil at the time of planting was below 1·5 MPa, which corresponded to 3·6 % (w/w) in July 1997, 5·7 % in September 1997, and 4·3 % in 1998. The soil moisture content for the moist blocks at the time of planting was 0·04 to 0·02 MPa (15·9 %, w/w, in July 1997; 18 % in September 1997; and 21 % in 1998). Weather data were obtained from an AgriMet weather station (Northwest Cooperative Agricultural Weather Network, Bonneville Power Administration, and the US Bureau of Reclamation, Boise, Idaho) located near the plot. The mean daily air temperature was 18 °C in July 1997, 15 °C in September 1997, and 21 °C during the 1998 experiment. In July 1997, rain was measured on days 35; the total rainfall was
5 mm. Rain was frequent during the experiment in September 1997. Measurable rain fell daily from 3 days after planting, to 8 days after planting; the total rainfall during that period was
75 mm. July 1998 was dry, with only one light rain event (1 mm) during the experimental period.
Bean seeds (cv. Bush Blue Lake 274) were surface disinfected, and incubated at room temperature on sterile moist paper towels overnight. The three bacterial treatments were: (1) Pf-5R (1x1010 c.f.u. ml1), (2) Pf-5R rpoS (1x1010 c.f.u. ml1), and (3) a 1 : 1 mixture of Pf-5R and Pf-5R rpoS (each strain at 5x109 c.f.u. ml1). Bacteria were applied by soaking bean seeds for 10 min in cell suspensions. Immediately after treatment, bean seeds were planted in moist and dry soil plots that were arranged in a randomized complete block design with five (1997) or six (1998) replications per bacterial strain per soil treatment. Each replicate plot contained 100 treated bean seeds in a 2 mx0·25 m area, surrounded by a 1 m zone of fallow soil. After planting, moist-soil plots were watered every 2 days. In July 1997, dry-soil plots were watered 6 days after planting. In 1998, dry-soil plots were watered 7 days after planting.
Periodically, five seedlings per plot were harvested by placing the seeds and individual root systems with adhering soil in 5 or 10 ml 10 mM phosphate buffer, and subjecting samples to vibrations in an ultrasonic cleaner for 5 min. Culturable populations of bacteria from the surface of bean seedlings were estimated by spreading dilutions on PAF containing 100 µg rifampicin ml1, or PAF 100 µg containing rifampicin ml1 and 50 µg kanamycin ml1, for recovery of Pf-5R or Pf-5R rpoS, respectively. Cycloheximide (50 µg ml1) was added to each medium to inhibit fungal growth.
Data analysis.
Mean population size of bacterial strains and standard error were calculated by averaging the log10 c.f.u. obtained from each sample. For samples with a non-detectable bacterial population size, a value of the detection limit minus 1 c.f.u. was assigned to the sample. The relative area under the population curve (RAUPC) was calculated for bacterial populations recovered from plant surfaces to compare differences in growth of bacterial strains (Stockwell et al., 2002). The area under the population curve (AUPC) was calculated for each trial with the following formula:
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For analysis of data from laboratory experiments assessing stress tolerance, survival ratios were calculated by dividing the observed population at a sample point by the initial population. Survival ratios were log10 transformed prior to statistical analyses. All population data, including RAUPC, were subjected to analysis of variance and mean separation by Fisher's protected least significant difference test at P=0·05 using Statistical Analysis Systems (SAS Institute).
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RESULTS |
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Desiccation stress was imposed in two ways: by placing cell suspensions on filter paper in sealed chambers with controlled humidity, and on glass surfaces dried more rapidly in a transfer hood. Pf-5 and the rpoS mutant maintained high populations for 6 h on filter papers maintained in sealed chambers at 100 % relative humidity (data not shown). At 75 % relative humidity, however, culturable populations decreased as the filters dried (Fig. 1c). Exponential-phase cells of Pf-5, and stationary-phase cells of the rpoS mutant, were significantly more sensitive to desiccation at 75 % relative humidity than were stationary phase cells of Pf-5 (Fig. 1c
). When dried more rapidly on glass surfaces, populations of stationary-phase cells of Pf-5 or the complemented mutant Pf-5 rpoS+ decreased by 3 log units during the 65 min drying period, whereas populations of exponential-phase cells of Pf-5, and cells of the rpoS mutant, decreased by
5 log units (Fig. 1d
). If cells of Pf-5 and the rpoS mutant were suspended in trehalose before drying on glass squares, they maintained stable culturable population sizes (data not shown). After 2 h exposure in a transfer hood, culturable populations of Pf-5 and Pf-5 rpoS suspended in trehalose decreased a mean log survival ratio of 1·1; whereas culturable cells of either strain suspended in water before drying were not detected (log survival ratio <6·5, data not shown).
Cells of Pf-5 and Pf-5 rpoS were tolerant of mild heat shock. After exposure to 42 °C for 5 or 20 min, culturable population sizes of Pf-5 and Pf-5 rpoS did not differ significantly from each other, or from the initial population size before treatment (data not shown).
When stationary-phase cells of Pf-5 were exposed to temperatures of 20 or 80 °C, culturable populations decreased from 2x108 to 1x107 c.f.u. ml1 (Fig. 2). Stationary-phase cells of the rpoS mutant were more sensitive than Pf-5 to low temperatures (Fig. 2
); among experiments, culturable populations of Pf-5 rpoS ranged from 2·5x104 to 6·3x106 c.f.u. ml1 after treatment at 80 °C, and from 3·2x102 to 1·3x105 c.f.u. ml1 at 20 °C. Exponential-phase cells of Pf-5 were more sensitive than stationary-phase cells to exposure at 20 or 80 °C; mean population sizes of survivors were
2x104 c.f.u. ml1. Similar to exponential-phase cells of Pf-5, exponential-phase cells of the rpoS mutant also were sensitive to freezing; mean populations of survivors ranged between 2x102 and 2x103 c.f.u. ml1 (Fig. 2
). In all experiments, survival of the complemented mutant Pf-5 rpoS+ did not differ significantly from survival of the wild-type strain. For stationary-phase or exponential-phase cells of all strains, treatment with trehalose prior to freezing resulted in no significant loss in culturability (Fig. 2
), presumably because of the cryoprotectant properties of trehalose (Zavaglia et al., 2003
).
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In experiments in 1997, Pf-5R and its rpoS mutant also were co-inoculated onto the same bean seeds. The patterns of population increase or decrease of co-inoculated strains were similar to those shown in Fig. 4 for single-strain inoculants (data not shown). The RAUPC of Pf-5R on beans in moist soil was significantly greater in each trial compared with Pf-5R or Pf-5R rpoS on seed in dry soil (Table 3
). Similar to results with single-strain inoculants, the RAUPC of Pf-5R rpoS on seed co-treated with Pf-5R was significantly lower than its co-inoculant in repeated experiments in moist soil conditions, and also on seed in dry soil in one of the two trials (Table 3
).
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DISCUSSION |
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The capacity to survive periods of desiccation is an important fitness factor in plant-associated bacteria, and the results of this study indicate that rpoS plays a key role in that capacity. This finding is consistent with previous observations that stationary-phase cells of Gram-negative bacteria are more desiccation resistant than are exponential-phase cells (Potts, 1994). In Pseudomonas putida, 14 of 35 genes identified on the basis of their induction under matric stress are preferentially expressed by stationary-phase cells rather than exponential-phase cells (Van de Mortel & Halverson, 2004
). Many of these water-deprivation-controlled genes are required for maximum survival of stationary-phase cells exposed to desiccation stress, an observation that prompted Van de Mortel & Halverson (2004)
to speculate that the genes may be under the control of rpoS. In response to desiccation, microbial cells undergo physiological changes that are likely to optimize their survival under these conditions. The physiological changes include the accumulation of disaccharides, such as trehalose, which serve as protectants of macromolecules within the cell; the accumulation of water-stress proteins; alterations in lipid composition of membranes to maintain membrane fluidity; enhanced production of extracellular polysaccharides; and morphological changes that maximize volume-to-surface ratios of bacterial colonies (Potts, 1994
). The mechanisms by which RpoS influences desiccation stress response of P. fluorescens are unknown, and could include the control of one or more of these physiological processes. Sigma factors other than RpoS also are known to play a role in the adaptation of P. fluorescens to desiccation stress. The sigma factor AlgU (also called AlgT, RpoE and
22), a key determinant of extracellular polysaccharide production, is required for optimal survival of P. fluorescens CHA0 when exposed to desiccation and osmotic stress (Schnider-Keel et al., 2001
). An rpoN mutant of CHA0 is also more sensitive than the parental strain to salt stress, indicating a role for
54 in osmotic stress (Péchy-Tarr et al., 2005
). It is likely that the desiccation stress response is a complex physiological process, components of which are subject to control by different regulatory circuits in Pseudomonas spp.
The results of this study also establish a significant role for rpoS in the capacity of Pf-5 to survive freezing and thawing. RpoS had a positive influence on the ability of Pf-5 to survive freezing at both 80 and 20 °C, temperatures that differentially influenced the survival of the cells. Trehalose served as a protectant for cells of Pf-5 exposed to freezing and to desiccation, a phenomenon that has been attributed to a dual mechanism of trehalose in other micro-organisms: stabilization of membranes and proteins by replacing water, and preservation of the intracellular water structure (Tanghe et al., 2003).
In addition to the role of rpoS in desiccation and freezing survival, which has not been reported in other Gram-negative bacteria, rpoS was required for optimal survival of UV irradiation by P. fluorescens Pf-5. This finding is consistent with the significant role of rpoS in survival of the plant pathogen Pseudomonas syringae when exposed to UV irradiation (Miller et al., 2001b). rpoS also was required for optimal survival of Pf-5 when exposed to starvation stress, and a similar role for rpoS was reported for P. putida KT2440 (Ramos-González & Molin, 1998
). In Pseudomonas aeruginosa, the role of rpoS in starvation stress response appears to be more subtle; it was found to influence the rate of die-off but not the final level of survival in one study (Jørgensen et al., 1999
), and to be affected by media in a second study (Suh et al., 1999
). An apparent discrepancy in the role of rpoS in stress response of different Pseudomonas spp. could be inferred by comparing studies evaluating survival of heat stress, with rpoS playing a significant role in survival of heat shock in P. aeruginosa (Jørgensen et al., 1999
; Suh et al., 1999
) that was not observed in the current study evaluating P. fluorescens Pf-5. This difference may be explained by different experimental conditions: P. aeruginosa was exposed to 50 or 53 °C in previous studies, whereas P. fluorescens was exposed to 42 °C in the current study, a temperature selected to approximate the maximum that Pf-5 might experience in a soil environment. rpoS is known to play a critical role in the oxidative stress response of P. aeruginosa (Jørgensen et al., 1999
; Suh et al., 1999
), P. fluorescens (Heeb et al., 2005
; Sarniguet et al., 1995
) and P. putida (Miller et al., 2001a
; Miura et al., 1998
), and the osmotic stress response of P. aeruginosa (Jørgensen et al., 1999
; Suh et al., 1999
) and P. fluorescens (Sarniguet et al., 1995
). While strain differences are likely to be revealed in future studies, the current body of literature suggests a key role for rpoS in stress response across the diverse genus Pseudomonas.
In repeated growth-chamber and field trials, we found that an rpoS mutant of Pf-5 did not survive as well as the wild-type strain in the rhizosphere of beans planted in dry soil, whereas differences in survival of the strains were sometimes less pronounced in moist soils. Therefore, the role of rpoS in rhizosphere fitness of Pf-5 could be attributed in part to the central role of rpoS in stress response, in this case the survival of desiccation stress. Similar findings have been reported for other Pseudomonas spp., where the role of rpoS in fitness has been tested in microcosms. rpoS influenced root colonization by a strain of P. putida under competitive conditions from other microbes, whereas rpoS did not have a detectable influence on colonization of roots planted in a sterile soil and maintained under gnotobiotic conditions (Miller et al., 2001a). An rpoS mutant of P. putida KT2440 was more sensitive than the wild-type strain to stress imposed by the toxic compound m-methylbenzoate in soil (Ramos-González & Molin, 1998
). The population size of the rpoS mutant declined more than that of the wild-type strain in soil treated with this compound. In contrast, soil populations of KT2440 derivatives containing the TOL plasmid, which confers m-methylbenzoate degradation, were stable in the soil irrespective of rpoS. From these results, we suggest that the role of rpoS as a fitness factor of Pseudomonas spp. in the soil or rhizosphere is related in part to its role in surviving stresses that the bacteria encounter in these habitats.
The findings of this study contribute to an evolving view of rpoS as an essential component of stress response in Pseudomonas spp., a role commonly attributed to its stature as a central regulator influencing stationary-phase gene expression. rpoS is known to influence the expression of at least 100 genes in E. coli upon conditions of cellular starvation or stress (Ishihama, 2000). Similarly, in P. putida KT2440, rpoS controls the expression of more than 50 genes encoding peptides that are synthesized in cells after a short period of carbon starvation (Ramos-González & Molin, 1998
). The span of its control must certainly be one factor in the cross-protection that rpoS exerts in the stress response of stationary-phase cells. It must also be remembered, however, that certain protective mechanisms can enhance a cell's capacity to survive a number of individual stresses. For example, freeze-induced cellular injury is both specific (damage due to intracellular ice crystal formation) and shared with other types of stresses, such as desiccation, osmotic and oxidative stresses (Tanghe et al., 2003
). Because of the destructive effects of oxygen during desiccation and freezing, oxygen-scavenging mechanisms of the cell are likely to be critical to the survival of cells encountering dry environments or subzero temperatures (Potts, 1994
; Tanghe et al., 2003
). With oxidative damage heralded as the Achilles heel of stationary-phase bacterial cells (Nyström, 2004
), oxygen management is likely to play a central role in a cell's capacity to survive in the presence of diverse environmental stresses, such as those imposed by starvation, freezing and desiccation. In that regard, rpoS is known to play a critical role in the oxidative stress response of Pseudomonas spp. (Jørgensen et al., 1999
; Miller et al., 2001a
; Sarniguet et al., 1995
; Suh et al., 1999
). Future studies are needed to identify mechanisms involved in the cellular response to the diverse environmental stresses that bacteria encounter in natural environments, and the role of rpoS and other global regulators in the capacity of the bacteria to survive these stresses.
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ACKNOWLEDGEMENTS |
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Received 29 March 2005;
revised 16 June 2005;
accepted 25 June 2005.
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