Sulfoacetaldehyde is excreted quantitatively by Acinetobacter calcoaceticus SW1 during growth with taurine as sole source of nitrogen

Sonja Weinitschke, Katharina Styp von Rekowski, Karin Denger and Alasdair M. Cook

Department of Biology, The University, D-78457 Konstanz, Germany

Correspondence
Alasdair Cook
alasdair.cook{at}uni-konstanz.de


   ABSTRACT
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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
 
Eighteen enrichment cultures with taurine (2-aminoethanesulfonate) as the sole source of combined nitrogen under aerobic conditions were all successful, and 24 pure cultures were obtained. Only three of the cultures yielded an inorganic product, sulfate, from the sulfonate moiety of taurine, and the others were presumed to yield organosulfonates. Sulfoacetate, known from Rhodopseudomonas palustris CGA009 under these conditions, was not detected in any culture, but sulfoacetaldehyde (as a hydrazone derivative) was tentatively detected in the outgrown medium of nine isolates. The compound was stable under these conditions and the identification was confirmed by MALDI-TOF-MS. Most sulfoacetaldehyde-releasing isolates were determined to be strains of Acinetobacter calcoaceticus, and a representative organism, strain SW1, was chosen for further work. A quantitative enzymic determination of sulfoacetaldehyde and its bisulfite addition complex was developed: it involved the NAD-coupled sulfoacetaldehyde dehydrogenase from R. palustris. A. calcoaceticus SW1 utilized taurine quantitatively and concomitantly with growth in, for example, an adipate-salts medium, and the release of sulfoacetaldehyde was stoichiometric. The deamination reaction involved a taurine dehydrogenase. Enrichment cultures to explore the possible release of organophosphonates from the analogous substrate, 2-aminoethanephosphonate, led to 33 isolates, all of which released inorganic phosphate quantitatively.


The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of A. calcoaceticus SW1 and Klebsiella oxytoca TauN1 are respectively AY823621 and AY823620.


   INTRODUCTION
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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
 
Taurine (2-aminoethanesulfonate) (Fig. 1) is widely if disjunctively distributed in the biosphere (e.g. in mammals but not in plants), but degradation of the stable C–sulfonate (C–{mic1511285E001}) bond is limited to microbes (Huxtable, 1992). Correspondingly, many bacteria can utilize the compound in diverse metabolic situations (e.g. Cook & Denger, 2002). Some researchers have used the compound as simultaneous sources of carbon (and energy), nitrogen and sulfur for aerobes (e.g. Chien et al., 1999), which did not expose the different phenomena involved in utilization of carbon and nitrogen via sulfoacetaldehyde and inducible sulfoacetaldehyde acetyltransferase (Xsc) on the one hand (Cook & Denger, 2002; Denger et al., 2004a) and of sulfur via globally regulated taurine dioxygenase on the other (Eichhorn et al., 1997; Kertesz, 2000, 2001). The dissimilation of taurine in anaerobic respirations and fermentations emphasized the importance of Xsc in the degradative pathway(s) (Cook & Denger, 2002), and the two reactions which generate the sulfoacetaldehyde have been examined. Taurine : pyruvate aminotransferase (EC 2.6.1.77) was initially thought to be the more common reaction (Cook & Denger, 2002), but its catabolic role has been confirmed in only three organisms with sequenced genomes, Rhodococcus sp. strain RHA1 (Denger et al., 2004a), Silicibacter pomeroyi DSS-3 (Moran et al., 2004) and Rhodobacter sphaeroides 2.4.1 (K. Denger, unpublished; see also Novak et al., 2004). Taurine dehydrogenase was discovered first (Kondo et al., 1973), but only recently have its biochemical identity [taurine : ferricytochrome-c oxidoreductase (deaminating) (EC 1.4.2.-); TauXY] and its widespread distribution been established (Brüggemann et al., 2004).



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Fig. 1. Pathway that generates sulfoacetaldehyde from taurine in A. calcoaceticus SW1.

 
The widespread distribution of taurine dissimilation allowed a common transcriptional regulator, TauR, to be attributed to the degradative pathway, independent of the deamination used by the organism (Brüggemann et al., 2004; Denger et al., 2004a; Moran et al., 2004). In addition, a homeostatic mechanism to maintain the osmotic pressure in the cell following cleavage of the sulfonate moiety as sulfite was attributed to TauZ, a putative sulfate exporter (Rein et al., 2005).

Analysis of the sequence of the genome of Rhodopseudomonas palustris CGA009 revealed candidate genes to encode TauR and TauXY but not Xsc, and the organism was found to utilize taurine as a sole source of combined nitrogen under oxic and anoxic conditions (Denger et al., 2004b). R. palustris generated sulfoacetate quantitatively, and a sulfoacetaldehyde dehydrogenase was observed. It was concluded that taurine could be a major source of environmental sulfoacetate and that an exporter of sulfoacetate must be present in R. palustris (Denger et al., 2004b).

We thus proceeded to test whether taurine was commonly used by bacteria as a sole source of combined nitrogen concomitant with release of sulfoacetate. The results showed that release of an organosulfonate was common under these conditions, but that sulfoacetate was never observed and that sulfoacetaldehyde was often the product which was released.


   METHODS
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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
 
Materials.
The bisulfite addition complex of 2-sulfoacetaldehyde dihydrate (53 % in sodium sulfate) was synthesized from 2-bromoacetaldehyde dimethylacetal and sodium metabisulfite and identified by NMR and IR (Denger et al., 2001): we were unable to convert this material to free sulfoacetaldehyde by published methods (White, 1988; Cunningham et al., 1998), as reported elsewhere (Gritzer et al., 2003). Phosphonoacetaldehyde, which had been synthesized by H. B. F. Dixon (Cambridge, UK), was kindly made available by J. P. Quinn (Belfast, Northern Ireland, UK). Other chemicals were from standard catalogues.

Organisms, growth media, growth conditions, cell disruption and enrichment cultures.
R. palustris CGA009 was grown in 1 l cultures in 5 l Erlenmeyer flasks which were shaken at 30 °C in the dark. The growth medium was a 50 mM potassium phosphate buffer, pH 7·2, which contained 0·25 mM MgSO4, 10 mM DL-malate, 2 mM taurine and trace elements (Thurnheer et al., 1986), as well as a seven-vitamin mixture (Pfennig, 1978), which was added after autoclaving. Cultures were inoculated (1 %) from a growing culture in the same medium and incubated until the turbidity indicated a protein concentration of 10 mg protein l–1 (7–8 days), when taurine was added to a final concentration of 2 mM. The culture was harvested 24 h later (30 000 g, 15 min, 4 °C), washed in 50 mM potassium phosphate buffer, pH 7·2, containing 5 mM MgCl2 and used immediately or stored frozen (–20 °C). Cells at about 250 mg wet weight (ml wash buffer)–1 were disrupted by eight passages through a chilled French pressure cell at 140 MPa. Debris was removed by centrifugation (20 000 g, 3 min, 4 °C). The specific activity of the sulfoacetaldehyde dehydrogenase in the resulting crude extract was about 1·3 mkat (kg protein)–1.

The growth medium described above was used with minor modifications for enrichment cultures (5 ml in 50 ml tubes) with 2 mM taurine or 2 mM 2-aminoethanephosphonate (ciliatine) as sole source of nitrogen. The carbon source in different sets of enrichments was (i) 5 mM glucose plus 10 mM glycerol plus 10 mM DL-malate, (ii) 5 mM glucose plus 10 mM glycerol plus 10 mM succinate, (iii) 10 mM succinate, (iv) 5 mM adipate, (v) 5 mM benzoate, (vi) 20 mM acetate or (vii) 10 mM DL-lactate. Each set of enrichments was accompanied by controls containing either no added combined nitrogen or 2 mM NH4Cl instead of taurine or 2-aminoethanephosphonate. The inocula were derived from the aeration tank of the wastewater treatment plant in Konstanz, from forest soil or from agricultural soil; in each case, the material was shaken with several batches of 50 mM potassium phosphate buffer, pH 7·2, and decanted or centrifuged to remove any soluble nitrogenous material and inorganic solids. Inoculation left the cultures visibly turbid. Incubation for 1–3 days showed negligible growth in the negative controls and similar growth in both experiments and positive controls. After three passages in fresh medium, all cultures were plated on complex medium (Luria–Bertani medium; Gerhardt et al., 1994) and common colony morphologies were picked to fresh selective medium. A culture was considered pure when three cycles of homogeneous plates and growth in selective medium were attained. Organisms isolated to utilize 2-aminoethanephosphonate were transferred to medium suitable to detect the possible release of phosphate from the source of nitrogen. The two alterations were to buffer with 50 mM MOPS/NaOH, pH 7·2 (instead of phosphate), and to add 0·5 mM potassium phosphate as a source of phosphorus. Outgrown cultures were analysed for the presence and concentrations of 2-aminoethanephosphonate and inorganic phosphate.

Growth of Acinetobacter calcoaceticus SW1 was followed in 50 ml cultures in 300 ml Erlenmeyer flasks shaken in a water bath at 30 °C. The growth medium was as described for R. palustris (above), except that the carbon source was 5 mM adipate and no vitamins were needed. Samples (2 ml) were taken at intervals to measure turbidity and to determine the concentrations of protein, taurine and other organosulfonates and the ammonium and sulfate ions. When larger amounts of cells were required for enzyme assays, 1 l cultures in 5 l Erlenmeyer flasks were used. Rupture of harvested cells of A. calcoaceticus was essentially as described above, but only three passages through the French pressure cell were required.

Rhodococcus opacus ISO-5 was grown with taurine as the sole source of carbon and energy for growth, as described elsewhere (Denger et al., 2004a). Crude extract was generated (Denger et al., 2004a) to serve as positive controls for some enzyme assays.

Enzyme assays.
Taurine : pyruvate aminotransferase was assayed discontinuously in 1 ml reaction mixtures maintained at 37 °C in a water bath. The reaction mixture contained 50 µmol Tris/HCl, pH 9·0, 5 µmol MgCl2, 5 µmol taurine, 10 µmol pyruvate, 100 nmol pyridoxal 5'-phosphate and 0·3 mg protein, with which the reaction was started. Samples were taken at intervals and taurine and alanine were determined. Crude extract from Rhodococcus opacus ISO-5 was used as a positive control. The reaction mixture was adapted to assay any 2-oxoglutarate-dependent transamination (EC 2.6.1.55) by replacing pyruvate with 2-oxoglutarate. Alanine dehydrogenase was assayed by a standard method (Laue & Cook, 2000). Taurine dehydrogenase was assayed with either dichlorophenol indophenol (DCPIP) or beef-heart cytochrome c as the electron acceptor (Brüggemann et al., 2004). Sulfoacetaldehyde dehydrogenase was assayed photometrically as the reduction of NAD+ at 365 nm. The reaction mixture (1 ml) at about 22 °C contained 50 µmol Tris/HCl, pH 9·0, 5 µmol MgCl2, 4 µmol NAD+, 400 nmol sulfoacetaldehyde (as the bisulfite addition complex) and 0·1–0·3 mg protein, with which the reaction was started.

Analytical methods.
Growth was followed as turbidity (an OD580 of 1·0 was taken as equivalent to 250 mg protein l–1) and quantified as protein in a Lowry-type reaction (Cook & Hütter, 1981). Reversed-phase chromatography was used to quantify taurine, 2-aminoethanephosphonate, alanine or glutamate after derivatization with 2,4-dinitrofluorobenzene (Laue et al., 1997) or free sulfoacetaldehyde (presumably in equilibrium with the added bisulfite addition complex) after derivatization with 2-(diphenylacetyl)indane-1,3-dione-1-hydrazone (Cunningham et al., 1998). Sulfate was determined turbidimetrically as a suspension of BaSO4 (Sörbo, 1987). Ammonium ion was assayed colorimetrically by the Berthelot reaction (Gesellschaft Deutscher Chemiker, 1996). Sulfoacetate or inorganic phosphate was quantified by ion chromatography with suppression (Denger et al., 2004b). The identity of sulfoacetaldehyde was confirmed by matrix-assisted, laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in the negative ion mode with a matrix of 4-hydroxy-{alpha}-cyanocinnamic acid; these determinations were done under contract by K. Hollemeyer (Technical Biochemistry, Saarland University, Saarbrücken, Germany). Values of apparent Km (Kmapp) were derived by hyperbolic curve-fitting as cited elsewhere (Ruff et al., 2003). SDS-PAGE and staining were done by standard methods (Laemmli, 1970). Standard methods were used to establish Gram reaction, catalase and cytochrome c oxidase activity (Gerhardt et al., 1994). A 1·4 kbp fragment of the 16S rRNA gene of several organisms under study was amplified by PCR, sequenced and analysed essentially as described elsewhere (Brüggemann et al., 2004); primers 16S-27f and 16S-1492r (Weisburg et al., 1991) were used.

Determination of sulfoacetaldehyde with sulfoacetaldehyde dehydrogenase from R. palustris.
This enzyme was determined to have Kmapp values of 26 µM for sulfoacetaldehyde and 92 µM for NAD+, as well as 12 mM for NADP+. NAD+ was obviously the physiological electron acceptor, and the high affinity for sulfoacetaldehyde made the catalyst a candidate for a specific (see Results) and sensitive enzymic determination of sulfoacetaldehyde (Bergmeyer, 1983). The enzyme could be enriched to about 28 mkat (kg protein)–1 by two separations at different pH values on an anion-exchange column, but this preparation still contained about 10 proteins in similar amounts (SDS-PAGE). We found that the soluble fraction was adequate to measure sulfoacetaldehyde in cell-free culture medium of A. calcoaceticus, provided the medium did not contain malate, the carbon source for growth of R. palustris. It was also apparent that both the free sulfoacetaldehyde and the bisulfite addition complex were substrates for the enzyme. The reaction mixture (in 1·0 ml) contained 50 µmol Tris/HCl, pH 9·0, 5 µmol MgCl2, 4 µmol NAD+ and <=60 nmol sulfoacetaldehyde, and the value of A365 was confirmed to be stable. The enzyme preparation (0·3 mg protein in 10 µl) was then added and the increase in the value of A365 was followed. The reaction was complete in about 5 min. It was possible to do the reaction with about 1 mM sulfoacetaldehyde in the test and a long reaction time. Under these conditions, 1 mM sulfoacetate was detected by ion chromatography after the enzyme reaction had ceased. We thus had confirmation that the required reaction was being catalysed.


   RESULTS
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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
 
Sets of enrichment cultures
A total of 18 enrichment cultures was set up in four series to obtain micro-organisms which could utilize taurine as the sole source of combined nitrogen for growth. The cultures differed in inoculum and source of carbon added. Every enrichment culture was positive, and 24 prokaryotic isolates were obtained (Table 1) as judged by light microscopy. None was found to produce sulfoacetate (compare Denger et al., 2004b), and only three produced sulfate (compare Denger et al., 2004a), so 21 isolates apparently released organosulfonates from taurine, which was quantitatively utilized in all strains except SW3.


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Table 1. Isolates obtained to utilize taurine as sole source of combined nitrogen for aerobic growth

Inocula were obtained from agricultural soil (A), forest soil (F) or activated sludge (S). Mixtures (i) and (ii) are detailed in Methods. ND, Not done.

 
Sulfoacetaldehyde was readily assayable by derivatization, and nine isolates were tentatively shown to generate this compound (Table 1). Isolate SW2 seemed to generate the compound only in the presence of glucose as carbon source, and this behaviour was deemed too complex for the present study. Strain SW3 was also not examined further, because the nitrogen source was not utilized quantitatively. One bacterium, strain SW1, which was representative of seven morphologically indistinguishable isolates, was chosen for further work. The 16S rRNA gene of strain SW1 (and two similar organisms) was sequenced. The gene shared >98·9 % identity of position with all three sequences of the corresponding gene in the type strain of A. calcoaceticus. Simple taxonomic and physiological tests supported this identification (Table 1), so we presume the group of organisms, which were similar to strain SW1, was composed solely of strains of A. calcoaceticus. A. calcoaceticus SW1 was deposited with the DSMZ (Braunschweig, Germany) as DSM 16962. Strain SW1 apparently generated about 2 mol sulfoacetaldehyde (mol taurine)–1 (this apparent paradox is resolved below).

The organosulfonate generated by the other 12 isolates was at first unknown (Table 1); it was later shown to be isethionate (2-hydroxyethanesulfonate) (K. Styp von Rekowski, unpublished data). Three groups of organisms seemed to generate the compound. The largest group (eight organisms) was represented by strain TauN1, whose 16S rRNA gene was sequenced. This showed 99·8 % identity of position with the corresponding gene in the type strain of Klebsiella oxytoca, and its taxonomic and physiological properties supported this conclusion (Table 1). K. oxytoca TauN1 was deposited with the DSMZ as DSM 16963. A similar train of argument identified two strains as Pseudomonas putida, represented by strain TauN2 (Table 1). Two organisms were not identified.

A series of enrichment cultures yielded 33 isolates, all bacteria, able to utilize 2-aminoethanephosphonate as the sole source of combined nitrogen for growth. Each isolate utilized 2-aminoethanephosphonate quantitatively, but, in all cases, the phosphonate moiety was recovered quantitatively after growth as phosphate. There was no need to postulate an organophosphonate as an excreted product of metabolism of 2-aminoethanephosphonate.

Confirmation of the identity of sulfoacetaldehyde, its stability and reproducible quantification
We presumed that sulfoacetaldehyde would be labile, especially with the electron-withdrawing sulfonate moiety on the carbon atom adjacent to the aldehyde group. However, boiling the putative sulfoacetaldehyde in spent growth medium of A. calcoaceticus did not generate detectable sulfoacetate or alter the concentration of sulfoacetaldehyde, and the addition of several microlitres of fuming nitric acid also failed to generate sulfoacetate. The putative sulfoacetaldehyde was stable in growth medium.

Analysis of portions of growth medium by MALDI-TOF-MS in the negative-ion mode showed that neither negative controls nor taurine ([M–1]=124) contained sulfoacetaldehyde, whereas spent taurine medium contained no detectable taurine but a product ([M–1]=123) which confirmed the presence of sulfoacetaldehyde (M=124). The identification, however, offered no quantification of the product.

We generated a third, enzymic, determination of the product using sulfoacetaldehyde dehydrogenase (EC 1.2.1.-) from R. palustris (Fig. 2). The quantification was thus provided by the molar absorption coefficient of NADH, which has been in use for over 60 years, and we confirmed that the purity of our salt-contaminated synthetic material is about 53 % (see Denger et al., 2001). When tested at higher substrate concentrations, the enzymic formation of the expected concentration of sulfoacetate could be determined by ion chromatography. We found no compound which interfered with the reaction (acetaldehyde, phosphonoacetaldehyde, glyoxylate, glycolaldehyde, propionaldehyde and succinate semialdehyde were tested). Whereas the standard curve of the hydrazone derivative suggested that 2 mol sulfoacetaldehyde (mol taurine)–1 were formed (see above), the enzymic determination indicated unit stoichiometry (see below). We presume that our attempts to convert the bisulfite addition complex of sulfoacetaldehyde to the free aldehyde were inadequate (as observed elsewhere; Gritzer et al., 2003), because Cunningham et al. (1998) specify that the method only derivatizes the free aldehyde.



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Fig. 2. Standard curve for the enzymic determination of sulfoacetaldehyde.

 
Growth physiology of A. calcoaceticus SW1
Strain SW1 grew with a specific rate (µ) of 0·71 h–1 and a growth yield of 47 g protein (mol taurine-nitrogen)–1 in adipate-salts medium (Fig. 3a); the same growth yield was obtained with the ammonium ion as sole source of nitrogen (not shown) and confirms the quantitative incorporation of nitrogen into cell material (e.g. Cook, 1987). Utilization of the nitrogen source was concomitant with growth, as was the release of sulfoacetaldehyde (Fig. 3b). Within experimental error, 1 mol sulfoacetaldehyde (mol taurine)–1 was formed (Fig. 3b). The specific degradation rate of taurine was calculated from the specific growth rate and the molar growth yield as 4·2 mkat (kg protein)–1.



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Fig. 3. Growth of A. calcoaceticus SW1 in 10 mM adipate-salts medium with 2·5 mM taurine as the sole source of combined nitrogen. In (a), the protein content is shown against time. In (b), the levels of taurine ({blacktriangleup}), sulfoacetaldehyde ({blacksquare}) and the ammonium ion ({square}) are shown against protein content.

 
Taurine dehydrogenase
Two paths of deamination of taurine are known (see Introduction). No taurine : pyruvate aminotransferase, with the associated alanine dehydrogenase, was detected. The possibility was considered that {beta}-alanine : 2-oxoglutarate aminotransferase [nominally a taurine transaminase (EC 2.6.1.55)] was present, but no activity was detected. In contrast, inducible taurine dehydrogenase could be detected with DCPIP as the electron acceptor; the specific activity was about 0·4 mkat (kg protein)–1. No activity was detected with beef-heart cytochrome c as the electron acceptor, so we presume that a native cytochrome c is needed.


   DISCUSSION
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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
 
The genome-enabled prediction, that R. palustris could utilize taurine and release a sulfonate, was confirmed when sulfoacetate was formed quantitatively (Denger et al., 2004b). The present paper, designed to test the generality of this phenomenon in new isolates, has confirmed that most organisms release an organosulfonate under these conditions, but that sulfoacetate was not the sulfonate released (Table 1). We wondered whether the apparently analogous 2-aminoethanephosphonate would be subject to similar transformations. The 33 isolates, all bacteria, which utilized this compound as a sole source of nitrogen all converted the phosphonate quantitatively to phosphate. Clearly, just as the biochemistry and regulation of cleavage of sulfoacetaldehyde (EC 2.2.3.15) and phosphonoacetaldehyde (EC 3.11.1.1) differ (Ternan & Quinn, 1998; Ruff et al., 2003), so do the patterns of their degradation. Analogous experiments were done with cysteate, whose known initial reaction is deamination (Rein et al., 2005); only sulfate was recovered, never sulfopyruvate or sulfolactate (R. Gueta and A. M. Cook, unpublished data). The release of organosulfonates thus seems to be something characteristic of taurine metabolism.

The release of sulfoacetate by R. palustris was rationalized as both the NADH-yielding oxidation of the aldehyde to the acid and the irreversible conversion of the reactive aldehyde to the non-toxic acid (Denger et al., 2004b). The observation that sulfoacetaldehyde is neither labile nor especially reactive weakens the latter argument. The fact that many organisms need not carry out the oxidation at all, or even carry out a reduction to isethionate (K. Styp von Rekowski, unpublished data), also weakens the former argument. We suspect that the release of sulfoacetaldehyde into the environment is widespread, because bacteria able to dissimilate the compound have been observed (Lie et al., 1996).

The pathway by which strain SW1 obtains the ammonium ion is short, with only three steps, two of which are transporters (Fig. 1). The specific activity of the metabolic enzyme, also presumably membrane-bound (Brüggemann et al., 2004), was only 10 % of that required for growth, but the natural electron acceptor was unavailable. Both ATP-binding-cassette transporters [TC 3.A.1.17.1] and tripartite, ATP-independent transporters [TC 2.A.56.4.1] are presumed to be involved with the uptake of taurine in different organisms (Brüggemann et al., 2004), but the nature of the sulfoacetaldehyde exporter is unknown, as is that for sulfoacetate in R. palustris (Denger et al., 2004b). The putative sulfate exporter in e.g. Paracoccus pantotrophus is apparently novel (Rein et al., 2005), so we hope to identify the genes involved in transport and oxidation of taurine and export of sulfoacetaldehyde, as well as the regulation, by characterizing taurine dehydrogenase, the corresponding gene(s) and its surroundings and developing testable, sequence-derived predictions.


   ACKNOWLEDGEMENTS
 
We are grateful to Anja Dahler, Sonja Fraas and Anke Schwarzenberger for isolates and data generated in a practical course for advanced students. J. P. Quinn (Queen's University, Belfast) kindly shared his understanding of the metabolism of sulfonates and phosphonates with us, and supplied a sample of phosphonoacetaldehyde synthesized by H. B. F. Dixon (University of Cambridge, UK). The project was supported by the University of Konstanz and the LBS-Stiftung Wohnen und Umwelt.


   REFERENCES
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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
 
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Received 17 December 2004; revised 20 January 2005; accepted 21 January 2005.



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