The Skaggs Institute for Chemical Biology and the Department of Chemistry, The Scripps Research Institute, La Jolla, California 92037
The Skaggs Institute for Chemical Biology and the Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037
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ABSTRACT |
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Recently strategies have emerged to profile the activity of enzyme superfamilies in complex proteomes using affinity-tagged chemical probes (6). These active site-directed probes profile proteins on the basis of function rather than abundance and are therefore capable of distinguishing, for example, active proteases from their inactive zymogens and/or inhibitor-bound forms (7, 8). To date, most efforts to create activity-based proteomic probes have exploited well known affinity labels as reactive groups, resulting in the generation of distinct sets of reagents that profile serine hydrolases (7, 9) and subclasses of cysteine proteases (8, 10). Recently serine hydrolase-directed probes were used to generate enzyme activity profiles that classified human breast and melanoma cancer cell lines into subtypes based on tissue of origin and state of invasiveness (11), indicating that the information content achievable in activity-based proteomic experiments is of sufficient quantity and quality to depict higher order cellular properties.
To accelerate the discovery of activity-based proteomic probes for enzyme classes lacking cognate affinity labeling reagents, we have introduced a non-directed or combinatorial strategy in which libraries of candidate probes are screened against complex proteomes for activity-dependent protein reactivity (12, 13). Through a two-tiered strategy utilizing rhodamine-conjugated probes for rapid and sensitive target detection and biotin-conjugated probes for target isolation and molecular identification, members of a probe library bearing a sulfonate ester reactive group were found to label in an activity-based manner enzymes from at least six mechanistically distinct classes (13). During these studies, however, we noted that certain sulfonate targets evaded molecular characterization. These proteins tended to exhibit "difficult" properties, such as membrane association, context-dependent labeling, and/or co-migration with endogenous biotinylated proteins, that frustrated efforts to proceed from the stage of target detection to target identification. Accordingly we hypothesized that a method in which the target detection and target purification steps of activity-based proteomic experiments were consolidated might facilitate the characterization of such recalcitrant protein targets. Here we report the synthesis of a class of trifunctional chemical proteomic probes in which both rhodamine and biotin tags are coupled to a sulfonate ester reactive group, thereby permitting the simultaneous visualization and affinity isolation of activity-based protein targets by in-gel fluorescence scanning and avidin chromatography, respectively. Using these trifunctional probes, we report the molecular characterization of several protein targets previously resistant to characterization by the two-tiered strategy described above. These targets include the integral membrane enzyme 3ß-hydroxysteroid dehydrogenase/5-isomerase and two cofactor-dependent enzymes, platelet phosphofructokinase and type II tissue transglutaminase. Notably, the latter two enzymes were significantly up-regulated in the invasive estrogen receptor-negative (ER(-)) human breast cancer cell line MDA-MB-231 relative to non-invasive ER(+) cell lines MCF7 and T-47D.
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EXPERIMENTAL PROCEDURES |
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To a solution of carboxylic acid (2) (Bachem, Torrance, CA; 0.06 g, 0.120 mmol, 1.0 equivalents (eq)) in N,N-dimethylformamide (3 ml) was added 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (0.032 g, 0.170 mmol, 1.4 eq) and N-hydroxysuccinimide (NHS) (0.032 g, 0.280 mmol, 2.3 eq). After stirring for 12 h at 25 °C, the reaction mixture was poured into saturated aqueous NaHCO3 solution (5 ml), and the product was extracted with ethyl acetate (3 x 5 ml). The organic layer was washed with water (15 ml) and saturated aqueous NaCl (15 ml), dried (MgSO4), and concentrated under reduced pressure. The crude NHS ester (0.070 g, 0.120 mmol, 3.5 eq) was dissolved in methanol (2 ml) followed by the addition of 5-(biotinamido)-pentylamine (Pierce; 0.015 g, 0.034 mmol, 1.0 eq). After stirring for 2 h at 25 °C, the solvent was evaporated under reduced pressure, and the remaining residue was washed with ethyl acetate (2 x 4 ml), solubilized in a minimal volume of chloroform, and transferred to a clean glass vial, and the solvent was evaporated. The process was repeated to rid the desired biotinylated intermediate of excess reagents and byproducts, affording 3 as a white film (50%): MALDI-FTMS (DHB) m/z 801.4007 (C41H58N6O7S + Na+ requires 801.3980).
The 9-fluorenylmethoxycarbonyl protecting group was removed by the addition of morpholine (0.135 ml, 1.40 mmol, 100 eq) to a solution of 3 (0.012 g, 0.014 mmol, 1.0 eq) in N,N-dimethylformamide (1 ml). After stirring the reaction for 1 h at 25 °C, the solvent was removed under reduced pressure, and the product washed with ethyl acetate (2 x 3 ml). To a solution of the deprotected -amino intermediate (0.010 g, 0.015 mmol, 1.1 eq) in N,N-dimethylformamide (1 ml) was added 5-(and-6)-carboxytetramethylrhodamine, succinimidyl ester (Molecular Probes, Eugene, OR; 0.007 g, 0.014 mmol, 1.0 eq) and triethylamine (0.013 g, 0.130 mmol, 10.0 eq). After stirring at 25 °C for 2 h, the volatiles were removed by rotary evaporation, and high performance liquid chromatography (HPLC) purification afforded 4 (55%): MALDI-FTMS (DHB) 969.4870 (C51H68N8O9S + H+ requires 969.4902).
The -amino tert-butoxycarbonyl protecting group was then removed by stirring 4 (0.006 g, 0.006 mmol, 1.0 eq) in 4 N HCl/dioxane (1 ml) for 1 h at 25 °C followed by evaporation of the volatiles under a stream of nitrogen. To a solution of the deprotected intermediate (0.005 g, 0.006 mmol, 1.0 eq) in methanol (1 ml) was added 5 (12) (0.008 g, 0.017 mmol, 3.0 eq) and NaHCO3 (0.001 g, 0.012 mmol, 2.0 eq). After stirring for 4 h at 25 °C, the reaction was filtered, and the solvent was removed under reduced pressure. HPLC purification afforded the final product, the trifunctional phenyl sulfonate probe 1, or TriPS (40%): MALDI-FTMS (DHB) m/z 1179.5623 (C62H83N8O11S2+ requires 1179.5617).
Tissue Sample Preparation, Labeling, and Detection
Mouse tissues were Dounce-homogenized in 50 mM Tris-HCl buffer, pH 8, 0.32 M sucrose, and the membrane and soluble fractions were separated by high speed centrifugation (sequential spins of 22,000 x g (30 min, pellet = membrane fraction) and 100,000 x g (60 min, supernatant = soluble fraction). The membrane fraction was washed twice and resuspended in Tris buffer without sucrose. Protein samples (2 mg/ml) were treated with 5 µM rhodamine-tagged or trifunctional sulfonate probe (250 µM stock in dimethyl sulfoxide), and the reactions were incubated for 1 h at 25 °C before quenching with 1 volume of standard 2x SDS-PAGE loading buffer (reducing). Quenched reactions were separated by SDS-PAGE (30 µg of protein/gel lane) and visualized in-gel using a Hitachi FMBio IIe flatbed laser-induced fluorescence scanner (MiraiBio, Alameda, CA). Labeled proteins were quantified by measuring integrated band intensities (normalized for volume).
Cancer Cell Line Preparation
Breast cancer cell lines were grown to 80% confluency in RPMI 1640 medium (Invitrogen) containing 10% fetal calf serum and harvested, sonicated, and Dounce homogenized in 50 mM Tris-HCl, pH 8.0 (Tris buffer). After centrifugation at 100,000 x g (40 min), the supernatant was collected as the soluble fraction, adjusted to 2 mg of protein/ml with Tris buffer, and labeled as described above.
Enrichment and Molecular Characterization of Sulfonate-reactive Proteins
For affinity isolation of protein targets directly from tissue or cell line fractions, 8 mg of total protein was used as starting material (equivalent to
8 x 107 cells). Samples diluted to 2.5 ml with Tris buffer were labeled with the TriPS probe (5 µM) for 2.5 h at 25 °C and then applied to a PD-10 size exclusion column and eluted with 3.5 ml of Tris buffer. For unsolubilized membrane samples, Triton X-100 was added to a final concentration of 1.0%, and the samples were rotated for 1 h prior to passage over a PD-10 column and elution with Tris buffer with 0.1% Triton X-100. Desalted samples were fractionated by Q-Sepharose chromatography, and fractions containing the desired targets were affinity-isolated using avidin-agarose beads (Sigma) as described previously (7, 12). Affinity-isolated proteins were separated by SDS-PAGE, excised from the gel, and digested with trypsin. The resulting peptides were analyzed by matrix-assisted laser desorption mass spectrometry (Kratos Axima CFR MALDI-TOF instrument, Kratos Analytical, Chestnut Ridge, NY). The MS data were used to search public data bases to identify the sulfonate-labeled proteins.
Recombinant Expression of Enzymes in Eukaryotic Cells
cDNAs corresponding to each sulfonate target were purchased as expressed sequence tags (Invitrogen), sequenced, and transiently transfected into COS-7 cells following methods described previously (9). Transfected cells were harvested by trypsinization, resuspended in Tris buffer, sonicated, and Dounce-homogenized. The soluble fraction was separated by centrifugation at 100,000 x g (45 min), adjusted to 1 mg of protein/ml with Tris buffer, and labeled as described above.
Type II Tissue Transglutaminase (tTG) Activity Assay
Soluble fractions of breast cancer cell lines (1 mg of protein/ml) were pretreated with 4 mM CaCl2 and/or GTP as indicated for 20 min at 25 °C followed by treatment with 250 µM 5-(and-6)-carboxytetramethylrhodamine-cadaverine (Molecular Probes). After incubation for 1 h at 25 °C, the reactions were quenched with 1 volume of standard 2x SDS-PAGE loading buffer (reducing) and separated by SDS-PAGE (15 µg of protein/gel lane). Samples were then visualized by in-gel fluorescence. For determination of the IC50 value for GTP inhibition of tTG activity, inhibition curves were generated for three distinct protein bands cross-linked to the rhodamine reporter group, and the estimated IC50 values from these curves were averaged to provide the reported value.
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RESULTS |
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In the course of screening cell and tissue proteomes with rhodamine-tagged sulfonate probes, we detected several labeled protein activities for which molecular identities were sought. Although many of these sulfonate-reactive proteins could be affinity purified with biotinylated probes, permitting their identification by mass spectrometry methods (13), some sulfonate targets proved resistant to molecular characterization by these methods. These sulfonate-reactive proteins generally represented lower abundance targets that displayed one or more of the additional challenging properties: 1) membrane association, 2) context-dependent labeling, and/or 3) migration on SDS-PAGE in the vicinity of endogenous biotinylated proteins. For example, an analysis of a panel of human breast carcinoma cell lines uncovered two 7580-kDa phenyl sulfonate-reactive proteins enriched in the ER(-) invasive line MDA-MB-231: an 80-kDa protein that exhibited ATP-sensitive labeling (Fig. 1, single arrowhead) and a 75-kDa protein that displayed calcium-dependent labeling (Fig. 1, double arrowhead). Initial attempts to label and affinity-isolate these proteins with biotinylated probes, either directly from the crude cytosolic preparation or following prefractionation by Q anion exchange chromatography, were unsuccessful. One challenge facing these analyses was that the 7580-kDa sulfonate targets migrated in the vicinity of endogenous biotinylated proteins (7, 14), complicating target detection by avidin blotting methods. Additionally we noted that both the 75- and 80-kDa proteins were unreactive with the phenyl sulfonate probe following desalting of the crude proteomic preparation (Fig. 2A), indicating that these proteins required additional cytosolic factors to maintain activity. This context-dependent reactivity displayed by the 7580-kDa proteins precluded their enrichment by chromatography methods prior to probe labeling. Attempts to label cytosolic preparations with biotinylated sulfonate probes prior to Q chromatography and then analyze the resulting column fractions by avidin blotting were hindered by the limited sensitivity, dynamic range, and throughput of this screening method (data not shown). To circumvent these shortcomings, a trifunctional probe was synthesized in which both rhodamine and biotin substituents were coupled to the phenyl sulfonate ester reactive group (TriPS, 1, see Scheme 1). We anticipated that this TriPS probe would allow us to track probe-labeled proteins through fractionation and purification protocols using in-gel fluorescence scanning, a method that offers greater sensitivity, dynamic range, and throughput relative to avidin blotting methods (1315). Additionally, fluorescence detection was expected to assist in identifying sulfonate targets in regions of the SDS-PAGE-fractionated proteome, like the 7580-kDa range, that are complicated by the presence of endogenous biotinylated proteins.
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The TriPS probe showed an overall proteome reactivity profile similar to the original rhodamine-tagged phenyl sulfonate probe (PS-rhodamine), labeling both the 75- and 80-kDa targets of interest (Fig. 2A). The labeling intensity of these proteins by the TriPS probe was moderately reduced compared with the reactivity observed with the parent PS-rhodamine probe, possibly due to the increased steric bulk of the trifunctional agent. Treatment of MDA-MB-231 cytosol (2.5 ml at 3.5 mg/ml) with the TriPS probe, followed by Q chromatography and SDS-PAGE analysis of the resulting fractions, provided a straightforward method by which to visualize fractions that were enriched for the labeled 75- and 80-kDa targets (Fig. 2B). These fractions were combined and treated with avidin-agarose beads as described previously (7, 12). Elution of bound proteins by heating in 1 volume of standard SDS-PAGE loading buffer provided a greatly enriched sample of TriPS-labeled targets (Fig. 2B). Protein bands corresponding to the 75- and 80-kDa targets were excised from the gel, digested with trypsin, and analyzed by matrix-assisted laser desorption ionization (MALDI) mass spectrometry, resulting in their identification as tTG and platelet-type phosphofructokinase (pPFK), respectively. A more detailed characterization of these sulfonate targets is described below. Collectively these results highlight the value of trifunctional chemical probes as tools that simplify the transition from target detection to target identification in activity-based proteomic experiments.
Purification and Characterization of Membrane Proteins with Activity-based Chemical Proteomic Probes
To test whether the devised methods would apply to the isolation and characterization of membrane-associated as well as soluble proteins, we pursued the identification of a 40-kDa phenyl sulfonate target selectively expressed in mouse testis membranes (13). Following treatment with the TriPS probe, testis membrane proteins were solubilized with Triton X-100 and separated by Q chromatography (Fig. 3A). Fractions enriched in the 40-kDa sulfonate target were subjected to avidin-based affinity purification procedures, and the enriched protein was identified by MALDI peptide mapping as 3ß-hydroxysteroid dehydrogenase/5-isomerase-1 (3HSD1), an NAD+-dependent integral membrane protein found predominantly in the gonads and adrenal gland (16). To confirm that 3HSD1 represented the target of sulfonate labeling, the cDNA for this enzyme was transiently transfected into COS-7 cells. In comparison to mock-transfected cells, a 40-kDa protein was labeled exclusively in the membrane fraction of 3HSD1-transfected cells (Fig. 3B). Importantly, the sulfonate reactivity of both native and recombinantly expressed 3HSD1 was significantly reduced in the presence of 1 mM NAD+, indicating that probe labeling occurred in the active site of the enzyme (Fig. 3, B and C). A more detailed comparison of the sulfonate labeling profile of 3HSD1 with a panel of probes revealed a preferential reactivity with monoaromatic sulfonate probes (e.g. phenyl (either PS-rhodamine or TriPS), thiophene, or p-nitrophenyl) relative to those containing polyaromatic (e.g. naphthyl or quinoline) or aliphatic (e.g. methyl) binding groups (Fig. 3D). These data demonstrate that activity-based chemical proteomic methods are compatible with the isolation and characterization of membrane-associated protein targets.
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DISCUSSION |
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Two of the sulfonate targets identified in this study, pPFK and tTG, were significantly up-regulated in the invasive ER(-) human breast cancer cell line MDA-MB-231 relative to the noninvasive ER(+) lines MCF7 and T-47D. To our knowledge, neither of these proteins has been previously identified as a constituent of the MDA-MB-231 proteome. pPFK, also referred to as PFK type C, catalyzes the first committed step in the glycolytic pathway (25). Many tumor types demonstrate high levels of PFK activity, which appears to support their characteristically enhanced levels of aerobic glycolysis (26). Notably, pPFK has been shown to correlate with malignancy in ascitic tumor cells in mice (27), suggesting that this enzyme may represent a general marker of advanced stages of cancers. pPFK activity is regulated in a posttranslational manner by several cytosolic factors, including ATP, which acts as an allosteric inhibitor (17). Importantly, we observed that the sulfonate labeling of pPFK was also inhibited by ATP, indicating that this probe-enzyme reaction occurs in an activity-dependent manner.
Like pPFK, tTG is up-regulated in certain types of cancers, including drug-resistant cell lines like NCI/ADR (28) and PC-14 (29). Curiously, however, tTG is also associated with apoptosis where increased transglutaminase activity has been proposed to contribute to cell death (30, 31). Thus, it remains unclear why certain cancer cells would overexpress an enzyme that promotes apoptosis. One possible explanation, as has been suggested for the NCI/ADR line, is that these cells possess reduced intracellular calcium stores and therefore fail to activate tTG (29). Alternatively, however, recent evidence suggests that the activation of tTG in response to apoptotic stimuli may protect cells from death through the transamidation of the retinoblastoma gene product, which inhibits its degradation by caspases (32). Thus, the up-regulation of tTG in invasive cancer cells, like MDA-MB-231 cells, may contribute to an apoptosis-resistant phenotype. Regardless, we anticipate that the sulfonate probes described herein should serve as important new tools for profiling tTG activity in complex proteomes. In support of this notion, the sulfonate reactivity of tTG was purely activity-based with labeling being dependent on the presence of the physiological activator calcium and blocked by the natural allosteric inhibitor GTP.
In summary, trifunctional chemical probes offer significant advantages for the emerging field of activity-based proteomics as these reagents greatly facilitate the molecular characterization of probe-labeled targets. Previous methods have benefited from the sensitivity of fluorescent probes for target detection and the specificity of biotin-avidin interactions for target isolation, but the uncoupled nature of the visualization and identification steps has frustrated efforts to identify certain difficult protein targets, including low abundance, membrane-associated, and/or cofactor-dependent enzymes. Through linking both rhodamine and biotin tags to an active site-directed reactive group, trifunctional probes overcome this shortcoming by consolidating the fluorescence detection of labeled proteins with avidin-based affinity chromatography procedures. Using trifunctional probes, we identified several challenging protein targets, including the integral membrane enzyme 3HSD1 and two cofactor-dependent enzymes, pPFK and tTG, that were up-regulated in invasive breast cancer cells. Notably, in the case of both pPFK and tTG, natural allosteric regulators of enzyme activity were found to exhibit commensurate effects on probe labeling, highlighting the ability of chemical probes to provide an accurate readout of the functional state of enzymes in complex proteomes. As more activity-based chemical probes are developed through both directed (710, 14, 15) and combinatorial methods (12, 13), their trifunctional variants will likely play important roles in accelerating the discovery of new protein targets associated with discrete physiological and/or pathological states.
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ACKNOWLEDGMENTS |
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FOOTNOTES |
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Published, MCP Papers in Press, September 21, 2002, DOI 10.1074/mcp.T200007-MCP200
1 The abbreviations used are: MS, mass spectrometry; 3HSD1, 3ß-hydroxysteroid dehydrogenase/5-isomerase-1; TriPS, trifunctional phenyl sulfonate probe; ER, estrogen receptor; HPLC, high performance liquid chromatography; MALDI, matrix-assisted laser desorption ionization; NHS, N-hydroxysuccinimide; pPFK, platelet-type phosphofructokinase; PS-rhodamine, rhodamine-conjugated phenyl sulfonate probe; tTG, type II tissue transglutaminase; FTMS, Fourier transform MS; DHB, 2,5-dihydroxybenzoic acid.
* This work was supported by NCI, National Institutes of Health Grant CA87660 and by the California Breast Cancer Research Program, Activx Biosciences, and the Skaggs Institute for Chemical Biology.
S The on-line version of this article (available at http://www.mcponline.org) contains Supplemental Figs. 13.
¶ To whom correspondence should be addressed: Dept. of Cell Biology, The Scripps Research Inst., 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Tel.: 858-784-8633; Fax: 858-784-2798; E-mail: cravatt{at}scripps.edu
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REFERENCES |
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