From the Nijmegen Center for Mitochondrial and Metabolic Disorders, Radboud University Nijmegen Medical Center, Geert Grooteplein 10, 6500 HB Nijmegen, ¶ Department of Microbiology, Faculty of Science, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, ** Department of Pediatrics and Neurology, Radboud University Nijmegen Medical Center, Reinier Postlaan 4, 6525 GC Nijmegen, and
Neuromuscular Center Nijmegen, Department of Neurology, Radboud University Nijmegen Medical Center, Reinier Postlaan 4, 6525 GC Nijmegen, The Netherlands
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ABSTRACT |
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In a closely related species, Methanothermobacter marburgensis, it was shown that complexes of the primary metabolism that are coupled functionally can also interact physically. The purification of heterodisulfide reductase activity resulted in the purification of the heterodisulfide reductase protein complex that also contained the methylviologen-reducing hydrogenase complex (5). In M. thermautotrophicus, similarly it was speculated that the association into high molecular weight complexes in concentrated solutions of purified methyl coenzyme M reductase and F420-dependent hydrogenase proteins could also play a role in vivo (9). Electron microscopy studies of enzymes and complexes involved in methanogenesis of Methanococcus voltae and Methanosarcina mazei Gö1 showed the associations of methanogenesis enzymes into supercomplexes, named methanoreductosomes (10). These results corroborate the generally accepted idea that the protein content of a cell is not merely a pool of individual and inert proteins but that the proteome of a living cell is an intricate network of proteins that interact and communicate. So far, research on M. thermautotrophicus has focused mainly on the enzymes and enzyme complexes that are involved in methanogenesis, and therefore only limited data are available on interacting proteins involved in other cellular processes.
To explore interacting (and non-interacting) proteins of an organism on a proteome-wide scale, only a limited number of techniques are available. One established method is the use of blue native electrophoresis followed by SDS-PAGE (or BN-PAGE) (11). In this method, proteins and protein complexes are solubilized by a mild detergent, charged with Coomassie dye, and separated natively in a first dimension depending on charge, size, and shape. In a second dimension, proteins and protein complexes are reduced and denatured with SDS and subunits, and individual proteins are separated by molecular weight. So far BN-PAGE has been applied mainly to membrane-associated complexes of organelles (12, 13) sometimes after dialysis of protein preparations (11) and often in combination with the use of antibodies.
In this study we present the results of a proteome-wide examination of interacting and individual proteins of M. thermautotrophicus using blue native/SDS-PAGE combined with mass spectrometry. This resulted in the identification of 361 proteins, corresponding to almost 20% of the predicted proteome, and visualization of a significant number of proteins that are part of enzyme complexes. These allowed, among others, the identification of an exosome-like complex of M. thermautotrophicus. Next to the homologs of four exosome core subunits identified previously (14), the M. thermautotrophicus exosome additionally contains a tRNA-intron endonuclease.
In summary, the results presented here give, for the first time, an overview of interacting and individual proteins of the archaeon M. thermautotrophicus on a proteome-wide scale using BN-PAGE. The genome coverage and the protein complexes identified by this technique clearly indicate the added value of including BN-PAGE in proteomic research and allow the study of protein complexes involved in primary metabolism, anabolism, and general cell processes.
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EXPERIMENTAL PROCEDURES |
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Sample Preparation
Before protein solubilization, the membrane fraction was washed three times with a solution containing 400 mM sorbitol, 25 mM NaCl, and 7.5 mM imidazole, pH 7.0, to reduce contaminating cytosolic proteins. Cytosolic and membrane protein preparations were diluted with 23 volumes of solubilization buffer (50 mM NaCl, 5 mM 6-aminocaproic acid, 1 mM EDTA, and 50 mM imidazole, pH 7.0) containing either 2% (v/v) laurylmaltoside, 1% (v/v) digitonin, or 1% (v/v) Triton X-100. After a 15-min incubation on ice with occasional vortexing samples were centrifuged for 30 min at 12,000 x g at 4 °C. Supernatants were transferred to clean tubes, and protein concentrations were determined with the 2D-Quant kit (Amersham Biosciences). Finally 4 µl of 750 mM 6-aminohexanoic acid with 5% (w/v) Serva Blue G was added per 100 µl of supernatant.
Chemical Cross-linking
To investigate the association of membrane-bound complexes, the proteins present in the membrane fraction were treated with the 8-Å linker dimethyl 3,3'-dithiopropionimidate dihydrochloride (Sigma) and the "zero-length" carbodiimide cross-linker 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDAC, Molecular Probes) in the presence of N-hydroxysulfosuccinimide (NHSS, Molecular Probes) (16). For this, an aliquot of 200 µl of the membrane fraction of MCR I cells was diluted with 600 µl of a solution containing 75 mM imidazole, pH 7, 400 mM sorbitol, 25 mM NaCl, and 0.03% laurylmaltoside. The chemical cross-linking reaction was started by adding 40 µl of freshly prepared 10 mg/ml dimethyl 3,3'-dithiopropionimidate dihydrochloride or 12 µl each of an 80 mM EDAC and 80 mM NHSS solution and proceeded for 1 h on ice with occasional mixing. The reaction was stopped by the addition of ammonium acetate to a final concentration of 0.1 M. As a control, immediately after addition of the cross-linkers, a 200-µl aliquot of the reaction mixture was quenched by adding 50 µl of 0.5 M ammonium acetate. Protein complexes were isolated immediately with either laurylmaltoside or digitonin as described above.
BN-PAGE and Gel Staining
Blue native/SDS-PAGE two-dimensional gel electrophoresis was performed as described elsewhere (13, 17, 18) and performed on a Protean II xi system (Bio-Rad). For the blue native first dimension, protein preparations were separated on gels containing the following bis-/acrylamide gradients: 413, 515, 618, 820, and 1022%. The ferritin monomer (440 kDa) and ferritin dimer (880 kDa) were included as markers. Samples were separated overnight at 15 °C at 100 V and typically finished after 16 h.
The second dimension was performed as described elsewhere (19) with some modifications. Individual lanes of the first dimension gel were excised with a razor blade and incubated in 1% (w/v) SDS and 1% (v/v) 2-mercaptoethanol for 12 h at room temperature. Next the first dimension lane, which was rinsed with milli-Q to remove excess 2-mercaptoethanol, was placed on top of a separation gel consisting of a 10% Tris-Tricine-SDS gel, which was cross-linked with piperazine diacrylamide. The flanking regions of the first dimension lane were filled with 10% native polyacrylamide gel. Using a notched inner glass plate, the Protean II xi system was converted into a four-gel system and started at a constant current of 5 mA per gel, which was raised to 10 mA after 45 min. Second dimension gels were run overnight at 15 °C and typically finished after 20 h. After the second dimension, gels were stained with colloidal Coomassie as described elsewhere (20) and scanned using an Amersham Biosciences Image Scanner.
Trypsin Treatment
After spot picking, protein present in gel plugs was first reduced by incubating for 10 min in 50 µl 10 mM DTT at 60 °C followed by an alkylating incubation of 45 min in 50 µl of 50 mM iodoacetamide at room temperature in the dark. Hereafter in-gel trypsin digestion was performed as described elsewhere (21) except that overnight trypsin treatment was performed in 5 µl of 50 mM ammonium bicarbonate containing 5 mM n-octyl glucopyranoside per plug. Next protein fragments were extracted by adding 1 µl of 0.5% (v/v) trifluoroacetic acid, 5 mM n-octyl glucopyranoside and incubating for 2 h at room temperature and a final 1-min sonication step. After extraction, peptides were stored at 20 °C until further analysis.
MALDI-TOF MS Measurements and MASCOT Search Parameters
For MS analysis, 0.25 µl of extracted peptides was pipetted on a MALDI-TOF sample plate and directly mixed with an equal volume of sample buffer containing 20 mg/ml -cyano-4-hydroxycinnamic acid in 0.05% (v/v) TFA, 50% (v/v) acetonitrile. MALDI-TOF MS measurements were performed in the mass range of 6502,600 Da on a Bruker III mass spectrometer, set to reflectron mode, after calibration using a mixture of bradykinin fragment 17, angiotensin, synthetic peptide P14R, and adrenocorticotropic hormone fragment 1839. Mass spectra were determined as the sum of 180 measurements. Monoisotopic peaks were manually selected, excluding background peaks. Peptide masses were exported to Biotools software and used to perform a MASCOT search in an in-house database of the M. thermautotrophicus predicted proteome. Search parameters allowed a mass deviation of ±0.3 Da, matching peptides containing one miscleavage, fixed modification of carbamidomethylated cysteines, and a variable modification of oxidized methionines.
Protein Identification Criteria
Proteins were regarded as identified proteins when the MASCOT search resulted in a Mowse score higher than 45 (corresponding to an expect score <104.5), which was calculated from the database size by the MASCOT software. A filter was included to recognize false-positives. A 2D gel electrophoresis study currently in progress has shown that proteins that are identified with matching peptides that mostly contain one miscleavage do not show a correlation between the predicted and experimental location on a 2D gel.2 Therefore, proteins were only regarded as significantly identified when they showed a Mowse score higher than 45 and contained mostly matching peptides that did not contain a miscleavage. Similar criteria applied for the identification of multiple proteins in a single spot. Here proteins were regarded as identified when peptides unique to that protein matched the above mentioned criteria.
LC-MS/MS Measurements and Protein Identification Procedure
All nanoflow LC-MS/MS experiments were performed on a 7-tesla Finnigan LTQ-FT mass spectrometer (Thermo Electron) equipped with a nanoelectrospray ion source (Proxeon Biosystems, Odense, Denmark). The LC part of the analytical system consisted of an Agilent Series 1100 nanoflow LC system (Waldbronn, Germany) comprising a solvent degasser, a nanoflow pump, and a thermostated microautosampler. Chromatographic separation of the peptides was performed in a 15-cm fused silica emitter (100-µm inner diameter; New Objective) packed in-house with methanol slurry of reverse-phase ReproSil-Pur C18-AQ 3-µm resin (Dr. Maisch GmbH, Ammerbuch-Entringen, Germany) at a constant pressure (20 bars) of helium. Then 5 µl of the tryptic peptide mixtures were autosampled onto the packed emitter with a flow of 600 nl/min for 20 min and then eluted with a 5-min gradient from 3 to 10% followed by a 25-min gradient from 10 to 30% acetonitrile in 0.5% acetic acid at a constant flow of 300 nl/min. The mass spectrometer was operated in the data-dependent mode to automatically switch between MS and MS/MS acquisition. Survey MS spectra (from m/z 350 to 2,000) were acquired in the FTICR with r = 50,000 at m/z 400 (after accumulation to a target value of 1,000,000). The three most intense ions were sequentially isolated for fragmentation in the linear ion trap using collisionally induced dissociation with normalized collision energy of 29% and a target value of 1,000. Former target ions selected for MS/MS were dynamically excluded for 30 s. Total cycle time was 3 s.
Proteins were identified via automated database searching (Matrix Science, London, UK) of all tandem mass spectra against both NCBInr and an in-house curated M. thermautotrophicus database. Carbamidomethylcysteine was set as fixed modification, and oxidized methionine and protein N-acetylation were searched as variable modifications. Initial mass tolerances for protein identification on MS and MS/MS peaks were 10 ppm and 0.5 Da, respectively. The instrument setting for the MASCOT search was specified as "ESI-Trap."
Protein Annotation and Similarities and Genomic Organization of Encoding Genes
The proteins identified were annotated on basis of the presence of domain signatures of the Pfam database (22). Sequence similarities were determined using Blast 2 sequences (23) at default settings. The organization of genes into operons was determined from the gene organization at the PEDANT website (24). Genes were considered to be part of an operon when the intergenic distance was smaller than 55 bp.
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RESULTS |
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Different first dimension gradients were applied to achieve maximum resolution of individual and complexed proteins. Typically individual proteins were optimally resolved with a first dimension gradient running from 10 to 20% bis-/acrylamide, whereas protein complexes showed maximum resolution with gradients starting at 4% bis-/acrylamide. Next to an optimization of the electrophoresis procedure and protein load, the identification of proteins also was optimized. The use of colloidal Coomassie staining in comparison to silver staining or traditional Coomassie staining enabled a high sensitivity while remaining completely compatible with mass spectrometry. Another improvement was the alkylation of cysteine residues after spot picking, which greatly reduced the number of background peaks after MALDI-TOF MS measurements and allowed the identification of multiple proteins in a single spot. An initial analysis of protein spots from duplicate gels and duplicate biological samples confirmed the reproducibility of BN-PAGE.
Identification of Proteins after BN-PAGE
The complete data set comprised the analysis of 1,550 protein spots by peptide mass fingerprinting. The data set included the optimization procedures, the appropriate reference protein spots from duplicate gels, and the identification of putative complex components originating from multiple blue native gels with different bis-/acrylamide gradients. The analysis showed that only a minor portion of the proteins identified appeared at multiple positions on gels and that no detectable protein degradation had taken place. The analysis of 875 protein spots of the proteins extracted from the membrane fraction allowed the identification of 300 different proteins, whereas the analysis of 675 protein spots from the cytosolic fraction identified 223 proteins. A total of 361 different proteins (for a reference map see Supplemental Fig. 1) were identified with an average Mowse score of 122 (which corresponds to an expect value less than 1012) and an average protein sequence coverage of 37% (see Supplemental Table 1). Five percent of the predicted membrane proteins (34 of 407 proteins containing one or more predicted transmembrane-spanning regions) were retrieved. This was also reflected in the coverages of the different protein function categories that were recognized after initial analyses of the M. thermautotrophicus genome (25). A relatively low coverage of proteins involved in transport or in membrane and cell wall synthesis was apparent. In contrast to the results for hydrophobic proteins, 22% of the total number of cytoplasmic proteins was recovered. These 327 proteins distributed more or less equally along the different protein function categories, and all of the previously characterized methanogenesis enzymes were identified. However, only a small number of hypothetical and conserved hypothetical proteins were recovered (34 proteins identified of 538 predicted). A higher coverage was obtained for enzymes involved in primary metabolism, amino acid metabolism, and other anabolic processes and included a relatively high coverage of ribosomal proteins (31 proteins identified of 63 predicted) and aminoacyl-tRNA synthetases (22 proteins identified of 26 predicted).
Identification of Homomeric Protein Complexes
A considerable amount of protein migrated as protein complexes. To identify true protein complexes, different first dimension gradients were applied to track complexes and to exclude any accidental co-migration of proteins. This was, however, limited to complexes that were resolved by gradients starting at 4% bis-/acrylamide or higher and not for complexes migrating at an extremely high apparent molecular mass (>1.5 MDa). A number of homomeric complexes were identified that were isolated previously from M. thermautotrophicus. Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin dehydrogenase migrated as a 200-kDa complex, corresponding with the molecular mass of 216 kDa of the hexameric complex as determined earlier (26). The most prominent protein present in cell-free extracts of M. thermautotrophicus, MTH1350,3 a F420-dependent oxygen detoxification flavoprotein, was purified previously as a 170-kDa homotetrameric complex (27). Here the protein complex could be clearly visualized migrating at an apparent molecular mass of 150 kDa. Next to the previously characterized complexes, a number of uncharacterized, putative homomeric complexes were recognized. These included an 800-kDa complex of the 15-kDa riboflavin synthase MTH1390 indicating an organization of this enzyme into a 50-mer, which is consistent with earlier reports (28, 29). A 700-kDa complex of the 73-kDa protein MTH632 containing a putative sugar kinase domain was identified, suggesting a decameric organization. A 300-kDa complex was identified for the 28-kDa MTH579 fructose-1,6-bisphosphate aldolase, suggesting an 812 multimeric organization of this protein (30). Similarly porphobilinogen synthase, MTH744, migrated as a complex of 300 kDa. This value corresponds with a homooctamer, and this quaternary organization is known to be intrinsic to the allosteric regulatory mechanism of these enzymes (31). A putative dimer and trimer migrating at 100 kDa were found, respectively, for MTH1476 encoding a homolog of the ß subunit of the tryptophan synthase and MTH1512 encoding a homolog of the H2-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase (Table I).
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Heteromeric Complexes Involved in Energy Generation
To evaluate BN-PAGE as a mild technique that can be used to identify protein associations, specific attention was given to the analysis of protein complexes involved in methanogenesis. Because of the extensive biochemical research that has been performed on methanogenesis, these complexes were regarded as internal standards. The use of BN-PAGE followed by mass spectrometry visualized and identified all enzyme complexes involved in methanogenesis (Table II).
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Next to the methanogenesis complexes (Table II), the archaeal ATP synthase was exclusively visualized in the membrane fraction by BN-PAGE. ATP synthase migrated as two separate subcomplexes containing either the membrane (A0) or catalytic part (A1) of the ATP synthase complex. Four protein bands corresponding to the A1 ATP synthase subunits A, B, D, and F migrated as one complex of 400 kDa. The membrane-embedded part or A0 segment of the ATP synthase migrated at a higher apparent molecular mass of 600 kDa and also contained four protein bands, which were identified as ATP synthase subunits C, E, H, and I. In contrast to the subunit topology determined for the Methanococcus jannaschii A0 and A1 ATP synthase subcomplexes, subunit C was only found in the A0 segment (6).
Supercomplexes
Interestingly A0 ATP synthase subunits I, C, and H were also identified migrating at 800 kDa and 1 MDa as multimers of the proton-translocating segment of the ATP synthase subcomplex (Fig. 2A).
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Heteromeric Complexes Involved in Anabolic Processes
Next to enzyme complexes involved in catabolism, complexes that are involved in biosynthesis also were identified. A complex that migrated with an apparent molecular mass higher than 1.5 MDa, which could just be resolved with a bis-/acrylamide gradient starting at 4% in the first dimension, was the core of the acetyl-CoA decarbonylase/synthase complex (7). It contained the subunits , ß, and
subunits (MTH1708, MTH1710, and MTH1713, respectively). The acetyl-CoA decarbonylase/synthase complex migrated with two proteins predicted to be involved in DNA repair and protein synthesis. Probably the observed associations of the proteins mentioned with the acetyl-CoA decarbonylase/synthase complex is due to the low resolving power at these extremely high apparent molecular weights.
Next to a complex involved in acetyl-CoA synthesis also a putative acetyl-CoA-converting complex was identified. The complex was composed of two proteins, MTH792 and MTH793, of which the encoding genes also constitute an operon. Both proteins appeared in a 1:1 ratio as was judged from their respective staining intensities. The MTH792 and MTH793 proteins are annotated as a 37-kDa 3-hydroxy-3-methylglutaryl-CoA synthase and a 40-kDa acetoacetyl-CoA thiolase, respectively, that consecutively would convert acetyl-CoA to 3-hydroxy-3-methylglutaryl-CoA, a precursor for lipid biosynthesis. The apparent molecular mass of 300 kDa of the complex suggests that it is composed of a tetramer of both subunits. Although this protein association has not been observed earlier, in radish seedlings, both activities are located in a single polypeptide (37).
Also MTH742 and MTH770 were found as a 300-kDa complex comprising the phenylalanine-tRNA synthetase and ß subunits, respectively. MTH1501 represents an additional copy on the genome of the
chain, which was not found associated with MTH770, suggesting that MTH742 is the true phenylalanine-tRNA synthetase
subunit in the preparations tested.
The 28-kDa MTH1149 and 45-kDa MTH1150 annotated as ATP-binding cassette transporter subunits Ycf16 and Ycf24 formed a complex of 200 kDa in a 1:1 ratio. Both encoding genes constitute an operon and encode two homologs of the iron uptake system, SufC and SufB, of Escherichia coli (56 and 41% sequence similarity, respectively) and Thermotoga maritima (61 and 48% sequence similarity, respectively). These proteins are involved in the protection of iron-sulfur clusters of proteins under oxidative stress conditions (38, 39) and have been shown to interact but so far not in Archaea.
Heteromeric Complexes Involved in General Processes
Complexes also were identified that are involved in general cell processes of M. thermautotrophicus, i.e. the proteasome and exosome involved in protein and RNA processing, respectively (40). A clearly visible complex was formed by the and ß subunits of the proteasome, MTH686 and MTH1202, respectively, migrating at an apparent molecular mass of 800 kDa (41). Interestingly the proteasome-activating nucleotidase protein MTH728 (42) was found in the same mass range as the proteasome but was not associated with the proteasome subunits (43, 44). MTH728 migrated as an 800-kDa complex with MTH540 in a 1:1 ratio. Although the MTH540 protein is annotated as a homolog of the Rad50 protein involved in DNA repair, the Rad50 signature was not recognized at the Pfam website in the MTH540 protein sequence and therefore is unlikely to be the homolog of the Rad50 protein in M. thermautotrophicus. Next to the proteasome complex and its regulatory protein, also the RNA-processing exosome complex was visualized at 900 kDa. In higher eukaryotes, this complex is involved in the processing of coding and non-coding RNA (45). The M. thermautotrophicus exosome complex consisted of four subunits corresponding to MTH682, MTH683, MTH684, and MTH891, which are the orthologs of the core subunits recognized previously in the Sulfolobus solfataricus exosome: Rrp42 (63% sequence similarity), Rrp41 (72% sequence similarity), Rrp4 (55% sequence similarity), and DnaG (68% sequence similarity), respectively (14). In analogy with the S. solfataricus exosome, the four core subunits appeared with similar staining intensities and were identical to the exosome preparation from S. solfataricus after immunoprecipitation of an anion-exchange column-enriched protein preparation (Fig. 3).
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DISCUSSION |
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Currently many techniques are used to study the protein content of organisms on a proteome-wide scale and to study the change of the proteome constituency depending on the environmental conditions applied. However, only a limited number of techniques are available to visualize and identify the interaction of proteins in vivo and on a proteome-wide scale. One established method of visualizing protein-protein interactions is the use of BN-PAGE (13). To date, this method is used to study specific complexes in combination with antibodies to identify proteins and to prove the multimeric nature of the protein complex under investigation (11).
Evaluating BN-PAGE as a Tool in Proteomics
In the study presented here we explored the use of BN-PAGE combined with mass spectrometry to visualize and identify interacting and individual proteins of M. thermautotrophicus on a proteome-wide scale. The obtained results show that BN-PAGE can compete with published proteome studies using traditional 2D gel electrophoresis and in fact surpasses the genome coverages obtained in most cases. Despite a relatively high coverage of predicted cytoplasmic proteins, parallel to traditional 2D gel electrophoresis (47), a low coverage was found for proteins containing one or more predicted transmembrane-spanning regions.
To visualize protein-protein interactions, the enzyme complexes involved in methanogenesis were used as internal standards because of the extensive biochemical research that has been performed on these enzyme complexes. All of the previously characterized protein complexes involved in the primary metabolism of M. thermautotrophicus were identified, indicating the mild nature of the method used, and furthermore showed that different bis-/acrylamide gradients in the first dimension could be used to exclude any accidental co-migration of proteins and as such identify members of uncharacterized complexes.
Supercomplexes
The association of complexes involved in methanogenesis that was observed earlier in M. marburgensis (5) also was reproduced by BN-PAGE. The heterodisulfide reductase complex was found as a separate complex and associated partly with the methylviologen-reducing hydrogenase complex under MCR I culture conditions. A comparison of the protein preparations obtained from cells cultured under MCR I and MCR II conditions with regard to the individual and associated forms of the heterodisulfide reductase complex showed considerable differences. Under MCR I conditions a considerable amount of the individual heterodisulfide reductase and the supercomplex were present that were hardly detectable under MCR II conditions. Apparently MCR I conditions induced heterodisulfide reductase and methylviologen hydrogenase components and furthermore triggered a physical link between these two complexes. One possible explanation might be that the amount and the association of both complexes of which one consumes reducing equivalents and the other complex supplies reducing equivalents would ensure that the reaction proceeded under low hydrogen (MCR I) conditions. These results furthermore suggest that, next to a regulation at the expression level, the association of complexes also might be part of the adaptations of the primary metabolism to a change in substrate supply.
"Homomeric" supercomplexes could be recognized for the membrane-associated methyltransferase complex and the ATP synthase stalk subcomplexes, respectively. The observation that ATP synthase complexes form multimers has only been observed for ATP synthases isolated from mitochondria (18) and chloroplasts (48) but not for bacterial ATP synthases (49). The multimerization of the M. thermautotrophicus ATP synthase subcomplex is therefore a unique observation for bacterial ATP synthases. However, to determine the exact function of the multimerization of the ATP synthase or the methyltransferase complex more research is required.
The present study has shown the ability of BN-PAGE to reproducibly separate individual proteins and reveal protein-protein interactions, consistent with the results obtained after independent, traditional purification procedures. Next to the identification of a considerable fraction of the predicted proteome, BN-PAGE can visualize the complexes involved in primary metabolism and in anabolic and general processes. Moreover BN-PAGE enables the comparison of the association of complexes between protein preparations obtained from cells cultured under different conditions. As such, BN-PAGE contributes considerably to the knowledge of the protein-protein interactions taking place and to the exact constituency of complexes that are involved in the major cell processes.
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ACKNOWLEDGMENTS |
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FOOTNOTES |
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Published, MCP Papers in Press, July 21, 2005, DOI 10.1074/mcp.M500171-MCP200
1 The abbreviations used are: MCR, methyl coenzyme M reductase; methyltransferase, N5-methyl-5,6,7,8-tetrahydromethanopterin:coenzyme M methyltransferase; BN-PAGE, blue native gel electrophoresis followed by SDS-PAGE; TES, N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid; EDAC, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide; NHSS, N-hydroxysulfosuccinimide; Tricine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine; 2D, two-dimensional.
2 M. H. Farhoud, J. C. T. Wessels, P. J. M. Steenbakkers, W. Pluk, E. Lasonder, I. Schmidt, R. A. Wevers, B. G. van Engelen, M. S. M. Jetten, J. A. Smeitink, L. P. van den Heuvel, and J. T. Keltjens, unpublished data.
3 MTH and number refers to the open reading frame number of the M. thermautotrophicus genome.
* Part of this work was supported by the Sixth Framework Program Priority 1, project titled "Rational treatment strategies combating mitochondrial oxidative phosphorylation (OXPHOS) disorders (Eumitocombat), Contract Number 503116.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
S The on-line version of this article (available at http://www.mcponline.org) contains supplemental material.
These authors contributed equally to this work.
|| To whom correspondence should be addressed: Dept. of Microbiology, Faculty of Science, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands. Tel.: 31-24-3652568; Fax: 31-24-3652830; E-mail: P.Steenbakkers{at}science.ru.nl
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REFERENCES |
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