Hypoxic Augmentation of Fast-Inactivating and Persistent Sodium Currents in Rat Caudal Hypothalamic Neurons

Eric M. Horn and Tony G. Waldrop

Department of Molecular and Integrative Physiology, Neuroscience Program, and College of Medicine, University of Illinois, Urbana, Illinois 61801


    ABSTRACT
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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

Horn, Eric M. and Tony G. Waldrop. Hypoxic Augmentation of Fast-Inactivating and Persistent Sodium Currents in Rat Caudal Hypothalamic Neurons. J. Neurophysiol. 84: 2572-2581, 2000. Previous work from this laboratory has indicated that TTX-sensitive sodium channels are involved in the hypoxia-induced inward current response of caudal hypothalamic neurons. Since this inward current underlies the depolarization and increased firing frequency observed in these cells during hypoxia, the present study utilized more detailed biophysical methods to specifically determine which sodium currents are responsible for this hypoxic activation. Caudal hypothalamic neurons from ~3-wk-old Sprague-Dawley rats were acutely dissociated and patch-clamped in the voltage-clamp mode to obtain recordings from fast-inactivating and persistent (noninactivating) whole cell sodium currents. Using computer-generated activation and inactivation voltage protocols, rapidly inactivating sodium currents were analyzed during normal conditions and during a brief (3-6 min) period of severe hypoxia. In addition, voltage-ramp and extended-voltage-activation protocols were used to analyze persistent sodium currents during normal conditions and during hypoxia. A polarographic oxygen electrode determined that the level of oxygen in this preparation quickly dropped to 10 Torr within 2 min of initiation of hypoxia and stabilized at <0.5 Torr within 4 min. During hypoxia, the peak fast-inactivating sodium current was significantly increased throughout the entire activation range, and both the activation and inactivation values (V1/2) were negatively shifted. Furthermore both the voltage-ramp and extended-activation protocols demonstrated a significant increase in the persistent sodium current during hypoxia when compared with normoxia. These results demonstrate that both rapidly inactivating and persistent sodium currents are significantly enhanced by a brief hypoxic stimulus. Furthermore the hypoxic-induced increase in these currents most likely is the primary mechanism for the depolarization and increased firing frequency observed in caudal hypothalamic neurons during hypoxia. Since these neurons are important in modulating cardiorespiratory activity, the oxygen responsiveness of these sodium currents may play a significant role in the centrally mediated cardiorespiratory response to hypoxia.


    INTRODUCTION
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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

Caudal hypothalamic neurons recorded in vivo from several different species including the rat, cat, and rabbit are activated during brief periods of hypoxia (Cross and Silver 1963; Dillon and Waldrop 1993; Ryan and Waldrop 1995). Since this region is well known to be involved in the modulation of cardiorespiratory function, we have hypothesized that these neurons contribute to the increased ventilation present with a decreased level of environmental oxygen (Horn and Waldrop 1997; Waldrop and Porter 1995). Evidence from this laboratory has supported the hypothesis by demonstrating that these neurons are inherently depolarized and show an increased firing frequency during briefs periods of hypoxia in vitro (Dillon and Waldrop 1992). Furthermore this membrane response appears to be due to a sustained inward current caused by an increased membrane conductance through tetrodotoxin-sensitive sodium channels (Horn et al. 1999).

Hypoxia has recently been shown to modulate several types of ion channels, including potassium, calcium, and sodium channels, at the whole cell level in vertebrate central neurons (Mironov and Richter 1998; Mironov et al. 1998; O'Reilly et al. 1997). In addition, single-channel analyses of these channels in neurons and other cell types have demonstrated that their properties are altered during periods of hypoxia (Fearon et al. 1997; Jiang and Haddad 1994; Ju et al. 1996). Of particular interest is the effect that hypoxia has on voltage-sensitive sodium channels since these channels are the primary effectors of neuronal excitability. The two main currents that flow through these channels are the fast-inactivating and persistent sodium currents (Brown et al. 1994; Chandler and Meves 1970; Keynes 1994). Both of these sodium currents are modulated by hypoxia in several cell types. In the human neocortex, fast-inactivating sodium currents decrease significantly when subjected to a hypoxic stimulus in vitro (Cummins et al. 1993). Furthermore fast-inactivating sodium currents in rat hippocampal neurons show a similar decrease in amplitude during hypoxia that is caused by an activation of protein kinase C (O'Reilly et al. 1997). Finally, single-channel analyses of sodium channels in rat ventricular myocytes has demonstrated that the persistent sodium current is enhanced during brief periods of hypoxia, while whole cell studies in rat neurons have shown both increases and decreases in this current during hypoxia (Hammarstrom and Gauge 1998; Ju et al. 1996; Kawai et al. 1999).

Since a functional enhancement of sodium currents is one possible cause of the depolarization and increased firing frequency seen during hypoxia in caudal hypothalamic neurons, the purpose of this study was to determine the effects of hypoxia on the specific properties of the two main sodium currents (fast-inactivating and persistent) in these neurons. To accomplish this goal, we voltage-clamped acutely dissociated caudal hypothalamic neurons in the whole cell patch-clamp configuration and recorded sodium currents during normoxic and hypoxic conditions. The results from these studies demonstrate an augmentation of both the fast-inactivating and persistent sodium currents during hypoxia in rat caudal hypothalamic neurons.


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METHODS
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Tissue preparation

All animal protocols and procedures described were performed in compliance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and the Guidelines of the Laboratory Animal Care Advisory Committee at the University of Illinois. Unless otherwise noted, all pharmacological agents were obtained from Sigma Chemical, St. Louis, MO.

Caudal hypothalamic neurons from 18- to 28-day-old male Sprague-Dawley rats (Harlan) were acutely dissociated using a modified procedure as previously described in hypothalamic and other rat neurons (Cummins et al. 1991; Kay and Wong 1986; Rhee et al. 1996; Uteshev et al. 1996). Briefly, the rats were anesthetized with vaporized halothane and rapidly decapitated. The brain from each animal was quickly removed, and the hypothalamus was blocked according to visual cues and placed in 100% oxygenated, iced artificial cerebrospinal fluid [ACSF (in mM) containing 120 NaCl, 1.25 NaH2PO4, 3 KCl, 2 MgCl2, 2 CaCl2, 25 Na+-HEPES, and 10 glucose at pH 7.2 and 295 mOsm, 4°C]. The hypothalamus was then cut into 500-µm-thick slices using a tissue chopper, and the slices were transferred into an incubation chamber containing ACSF at room temperature. The slices were incubated in ACSF containing 0.037% trypsin (Sigma, type XI) at room temperature for 20 min followed by three serial washes in ACSF. This was followed by another 20-min incubation in ACSF containing 0.025% protease (Sigma) at room temperature. The slices were then washed three times in ACSF and stored in the incubation chamber for no more than 6 h prior to recording.

Immediately prior to recording, an individual hypothalamic slice was removed from the incubation chamber and placed in a petri dish containing ACSF gassed with room air. Using a dissecting microscope, the caudal hypothalamus was carefully dissected from the rest of the hypothalamus using visual landmarks according to a stereotaxic atlas (Paxinos and Watson 1986). The tissue was then placed into a trituration solution (identical solution as ACSF except for the removal of CaCl2 and the addition of 10 mM EGTA and 2 mM kynurenic acid) and slowly triturated using a series of three diminishing bore Pasteur pipettes with fire-polished tips. The cell suspension was then plated onto a coated coverslip (Cell-Tak, Collaborative Biomedical Products) and placed into the recording chamber (RC-25, Warner Instruments) for 5 min prior to recording to facilitate adhesion to the recording surface.

Electrophysiological recordings

Individual neurons were visualized using an inverted microscope equipped with phase contrast optics (PIM, World Precision Instruments). According to previously described visual criteria, only those neurons deemed viable were recorded (Kay and Wong 1986; O'Reilly et al. 1997). Briefly, viable neurons were identified by the presence of a smooth membrane surface, nonprominent nuclear complex, three-dimensional shape, at least two projections (axonal and dendritic processes), and minimal swelling.

Whole cell recordings were made using single-electrode patch-clamping techniques (Hamill et al. 1981). Microelectrodes suitable for patch-clamping were pulled from borosilicate glass (PG52165-4, World Precision Instruments) using a gravity micropipette puller (PP-83, Narishige), and the tips were fire-polished to a tip resistance of 1-4 MOmega . The intracellular solution was specifically designed to isolate sodium currents and contained the following (in mM): 130 CsF, 10 TEA-Cl, 11 EGTA, 1 CaCl2, 2 MgCl2, 1 ATP, and 10 HEPES, pH 7.25, 275 mOsm. In a few neurons, the CsF was replaced with KF and the TEA-Cl removed to record normal membrane properties. Cells were approached using a motorized micromanipulator (DC-3K, Fine Science Tools) until a seal resistance of >= 2 GOmega was obtained. All recordings were acquired in the voltage-clamp mode using the AxoPatch 200A amplifier (Axon Instruments) with the currents filtered with a 4-pole Bessel filter (-3 dB and 5 kHz), digitized at 15 kHz, and stored on a computer for later analysis (PClamp 6.0.5 for acquisition and both 6.0.5 and 8.0.1 for analysis). In addition, the junction potential was nullified immediately prior to seal formation using the internal circuitry of the amplifier. When recording fast-inactivating sodium currents, the extracellular solution consisted of the following (in mM): 20 NaCl, 120 N-methyl-D-glutamic acid (NMDG), 3 KCl, 2 CaCl2, 2 MgCl2, 0.5 NiCl2, 0.1 CdCl2, 2 4-aminopyridine (4-AP), 10 TEA-Cl, 10 glucose, and 25 HEPES at pH 7.4 and 295 mOsm. When recording persistent sodium currents, the solution was the same as the ACSF except for the addition of NiCl2, CdCl2, TEA-Cl, and 4-AP in the same concentrations as the fast-inactivating sodium current solution. Additionally, several neurons were recorded using the standard ACSF to determine normal membrane properties. All solutions were perfused into the recording chamber at 1 ml/min.

Once a neuron was obtained in the voltage-clamp mode, the series resistance (>= 80%) and capacitance were compensated using the internal circuitry of the amplifier. Taking into consideration the large and rapid currents that flow through the sodium channel, several electrophysiological criteria were instituted to obtain acceptable and reliable recordings of these currents. First, no cells were used for data analysis if the current needed to hold the membrane at -100 mV exceeded 100 pA. We have found that cells needing a large current to maintain a negative holding potential have significant leaks and are unstable. Second, only cells that were obtained with a series resistance of <10 MOmega were studied because the voltage offset can be significant (>10 mV with an uncompensated series resistance of >10 MOmega when recording currents ~1 nA). Last, the initial activation curves (see following text) for voltage steps from a holding potential of -90 mV were compared with a holding potential of -60 mV to elucidate any voltage errors. If the activation curves differed by >5 mV, then the data for that cell was discarded.

Experimental protocol

Once a cell had been successfully patch-clamped, a series of voltage-step protocols were performed during normoxic conditions (chamber gassed with room air), depending on the type of current to be studied. For fast-inactivating currents, the activation and inactivation properties of each cell were analyzed. From a holding potential of -90 mV, depolarizing pulses of 60 ms were induced starting at -60 mV and continuing to +25 mV in 5-mV increments. The peak inward current at each depolarizing step was recorded, and the voltage dependence of activation was calculated using the conductance G at each step where G = Ipeak/Vstep - Vreversal. The reversal potential (Vreversal) was determined by taking a linear regression of the last four data points from the current/voltage plot from each protocol. The inactivation properties were determined by measuring the peak inward current generated by a 20-ms step depolarization to -10 mV from each of 200 ms prepulses from -100 to +10 mV in 10-mV increments from an initial holding potential of -90 mV. When analyzing the persistent component of the sodium current, two approaches were taken. First, long (60 ms) step depolarizations were instituted over the same range as with the fast-inactivating current, and the last 10 ms of current was quantified at each step to generate the current-voltage relationship. Second, depolarization ramps from -90 to +35 mV of various rates (0.5-5.0 mV/ms) were induced and the peak, persistent current measured for each rate. Each set of protocols was repeated for every cell at intervals of 2-3 min to elucidate any shift from baseline prior to testing during hypoxia. After the baseline currents were established, the chamber was perfused with a hypoxic solution that was identical to the control solution except that the hypoxic solution had been rigorously gassed with 100% N2 for >= 1 h and the oxygen scavenger Na2S2O4 (2 mM) was included. No shift in pH occurred with perfusion of the hypoxic solution compared with control perfusion. In some experiments, a Clark-style oxygen-sensing electrode (Model 737, Diamond General) and polarographic amplifier (Model 1900, AM Systems) were used to measure the absolute oxygen level in the chamber. The polarographic electrode was calibrated using four separate chambers of varied known oxygen partial pressure and generating a standard curve. At least 2 min was allowed for the hypoxic solution to saturate the chamber before the voltage protocols were repeated.

Data analysis

To eliminate any effects of a shift in activation between normoxic and hypoxic conditions, the peak fast-inactivating current was determined as the largest current over the entire range of voltage steps (usually between -20 and +10 mV) and not just at a single step level. This peak current was then compared between control and hypoxic conditions using a paired Student's t-test. A Boltzman fit of the data was used to obtain the activation curves for the fast-inactivating current as follows: G/Gpeak = 1/{1 + exponential[(V1/2-V)/k]}, where Gpeak is the peak conductance value, G is the conductance for each voltage step (see preceding text), V is the ending voltage level at each step, V1/2 is the half-activation value, and k is the slope constant. For the steady-state inactivation curves, the Boltzman fit was used as follows: I/Ipeak = 1/{1 + exponential[(V1/2-V)/k]} where Ipeak is the peak current, I is the current generated from the step depolarization to -10 mV from each prepulse step, V is each individual prepulse (see preceding text), V1/2 is the half-activation value, and k is the slope constant. For both the activation and inactivation analyses, the V1/2 values were compared between control and hypoxic conditions using Student's paired t-test. The activation kinetics were determined by measuring the time between the onset of the voltage pulse and the peak current response for each step. Inactivation kinetics were determined by fitting the current tracings at each step using a double exponential of the Chebyshev algorithm in Clampfit 8.0.1 (Axon Instruments).

Persistent current for the long step depolarizations was measured as the average current over the final 10 ms of the step at each voltage level. Again to eliminate any effect from a shift in activation, the peak current over the entire range for each protocol was used as comparison between normoxic and hypoxic states. The persistent current was measured during the ramp protocols as the single peak current at each ramp rate after excluding any possible fast-inactivating current. The persistent current at each rate was then compared between control and hypoxic conditions with the common rate of 1 mV/ms used to compare the sample populations due to the consistent nature of this rate to generate only persistent and not fast-inactivating currents. Data are presented as means ± SE, and the significance level for each comparison was determined to be P < 0.05.


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INTRODUCTION
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Recordings were made from a total of 61 caudal hypothalamic neurons with separate populations being used for analysis of the fast-inactivating (n = 28) and persistent (n = 26) sodium currents. All of the neurons studied had at least two projections extending from their soma, and most were of an either bipolar or multipolar morphology. The typical appearance of a multipolar caudal hypothalamic neuron just prior to seal formation is shown in Fig. 1A.



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Fig. 1. Example of a multipolar caudal hypothalamic neuron following acute dissociation (A). When recorded in artificial cerebrospinal fluid (ACSF), all neurons tested had membrane properties similar to previous reports (Dillon and Waldrop 1992; Horn et al. 1999). B: recording demonstrates an action potential amplitude of 98 mV and input resistance of 194 MOmega when recorded in current-clamp mode. The current-voltage relationship (top) was elicited with 200-ms pulses in 20-pA increments (-100 to +20 pA) during hypoxia (only 40 pA increments are displayed for optimal visualization). Bottom left: the increased conductance of this cell during hypoxia (normoxia RN = 422 MOmega ; hypoxia RN = 274 MOmega ), while the action potential (bottom right) was elicited with a 100-pA, 50-ms pulse. Scale bar in A = 5 µm. A and B were from different cells.

Basal membrane voltage properties (resting membrane potential = -49.0 ± 3.8 mV, input resistance = 425.0 ± 98.0 MOmega , action potential amplitude = 100.2 ± 6.7 mV, and action potential half-width = 0.96 ± 0.1 ms) were determined using protocols in the current-clamp mode from seven separate neurons recorded in normal ACSF. The membrane properties of these cells were similar to previous studies of caudal hypothalamic neurons in a brain slice preparation (Horn et al. 1999), indicating that no significant changes occurred due to the dissociation. One example of the membrane responses of a caudal hypothalamic neuron to depolarizing and hyperpolarizing pulses is shown in Fig. 1B. This cell demonstrated an increased conductance during hypoxia as seen in the I-V plot. Basal properties were not determined from the other 54 neurons since the extracellular and pipette solutions needed to isolate the separate sodium currents distorted these measurements.

The oxygen level in the recording chamber, measured with the polaragraphic electrode, rapidly decreased to 10 Torr within 2 min of switching the medium and reached a steady state <= 5 Torr within 4 min from a baseline of ~160 Torr (Fig. 2). This level of hypoxia has previously been shown to elicit a rapid depolarization and increase in firing frequency in caudal hypothalamic neurons in a brain slice preparation (Horn et al. 1999).



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Fig. 2. Graph depicting the level of oxygen delivered to the acutely dissociated caudal hypothalamic neurons during a 6 min wash with 100% N2 saturated solution containing 2 mM Na2SO3 (n = 6 trials). Means ± SE.

Fast-inactivating sodium currents

The averaged results for maximal fast-inactivating sodium current elicited by a depolarizing pulse from a holding potential of -90 mV was 956.9 ± 149.2 pA in the 11 neurons tested with the sodium level reduced by >85%. This current was significantly enhanced (1258.2 ± 242.8 pA) during the period of hypoxia as shown in Fig. 3. When TTX (2 µM) was introduced into the bathing medium, the entire fast-inactivating current was abolished (data not shown), suggesting that the currents analyzed were exclusively TTX-sensitive sodium currents. Normoxic conditions were restored in 6 of 11 of the cells following the hypoxic period to determine if the changes noted were due to a shift in the baseline, which can arise from intracellular washout in the patch-clamp configuration. In these cells, the initial normoxic maximal fast-inactivating sodium current of 1,138.2 ± 191.9 pA was significantly enhanced during hypoxia (1,498.8 ± 348.5 pA) and subsequently returned toward the normoxic level (1,339.7 ± 287.4 pA) when tested in the control conditions following hypoxia. An example of the recovery of the fast-inactivating current following the hypoxic perfusion is shown in Fig. 3.



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Fig. 3. Example of fast-inactivating sodium currents elicited by depolarizing voltage steps from -90 mV in a caudal hypothalamic neuron during normoxic, hypoxic, and recovery conditions.

The peak fast-inactivating sodium current was accentuated during hypoxia throughout the entire activation range as shown in Fig. 4. This figure illustrates the rationale for using the peak sodium current at the voltage step where the greatest current was elicited (mainly between -10 and 0 mV). Because the activation currents shifted during the hypoxic period, using a fixed voltage step value (such as -90 to -10 mV) would have failed to accurately compare the fast-inactivating current between the normoxic and hypoxic conditions. The activations of these currents were analyzed in more detail by calculating the conductance ratio at each step and fitting the data to the Boltzman equation. As shown in Fig. 5, the V1/2 value was significantly shifted to a more hyperpolarized level during the hypoxic stimulation (-17.9 ± 1.6 vs. -21.8 ± 2.0 mV, P < 0.05), indicating a lowering of the activation threshold and a greater ability to generate an action potential at hyperpolarized membrane potentials (Werkman et al. 1997). The steady-state inactivation was also analyzed using the Boltzman fit to the current ratio at each step throughout the entire inactivation range. Figure 6 shows that the inactivation curve during hypoxia was significantly shifted to a more negative value when compared with the normoxic conditions (V1/2 = -51.4 ± 1.6 vs. -54.4 ± 2.0 mV, P < 0.05), indicating that hypoxia leads to a greater inactivation at hyperpolarized membrane voltages.



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Fig. 4. Averaged results (±SE) from 11 caudal hypothalamic neurons demonstrating how hypoxia enhances the fast-inactivating current over the entire range of activation. Each step (-60 to +25 mV) was elicited from a holding potential of -90 mV.



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Fig. 5. Steady-state activation curves (means ± SE) from 11 caudal hypothalamic neurons during normoxic and hypoxic conditions. The half-maximal voltages (V1/2) were obtained by fitting the data points (G/Gmax - conductance/maximal conductance) to a Boltzman curve (*P < 0.05).



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Fig. 6. Steady-state inactivation curves (means ± SE) from 11 caudal hypothalamic neurons during normoxic and hypoxic conditions. The half-maximal voltages (V1/2) were obtained by fitting the data points (I/Imax - current/maximal current) to a Boltzman curve (*P < 0.05). Inset: the inactivation protocol during normoxia in 1 cell.

The kinetics of activation and inactivation were analyzed to determine any effect of hypoxia on these properties. As shown in Fig. 7A, the kinetics of inactivation were best fit using a double exponential that revealed a fast and slow component. When combined, the inactivation kinetics were not affected by hypoxia in these cells (Fig. 7B) nor were either the fast or slow components affected by hypoxia throughout the entire inactivation range (Fig. 7C). Likewise the activation kinetics of these neurons were unaffected by hypoxia throughout the entire range of activation as shown in Fig. 7D.



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Fig. 7. A: fast inactivation of sodium current in caudal hypothalamic neurons is composed of a fast and slow component. B: when scaled to the hypoxic current, the combined time constant of inactivation is unaffected. C: both the fast and slow time constants of inactivation are also unaffected by hypoxia throughout the entire voltage range. D: the kinetics of activation are similar between normoxia and hypoxia. For details, see text. Means ± SE.

Persistent sodium current

The persistent sodium current in the caudal hypothalamic neurons was differentiated from the fast-inactivating current by two methods. First the cells were slowly depolarized from -90 to +35 mV using a ramp protocol. Figure 8A demonstrates the distinction between the fast-inactivating and persistent currents using this method. The faster ramp rate (2.0 mV/ms) elicited a fast-inactivating current with the persistent current remaining after the fast-inactivating current ceases following inactivation. When the ramp depolarization rate was slowed to 0.67 mV/ms, the inactivation of the fast-inactivating sodium current is elicited at the same time as the activation of this current thus only the persistent sodium current was observed. The average persistent current elicited (at a ramp rate of 1 mV/ms) in 11 caudal hypothalamic neurons perfused with the low sodium solution was 114.7 ± 23.0 pA. This current was significantly increased to 162.5 ± 27.6 during the hypoxic stimulus (Fig. 8C). When the control solution was perfused into the chamber following the hypoxic period, the persistent current returned to near normal levels (Fig. 8B). Inclusion of TTX (2 µM) in the chamber (Fig. 8B) abolished most of the persistent current; however, a small (<50 pA) TTX-insensitive component remained that was minimally apparent above the level of the noise in the recordings.



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Fig. 8. Persistent current (A) elicited by a ramp depolarization (-90 to +35 mV) at 2 rates (2 and 0.67 mV/ms). Note the disappearance of the fast-inactivating sodium current at the slower ramp rate. B: example of a hypoxia-induced increase in the persistent sodium current in 1 caudal hypothalamic neuron that is blocked by application of TTX (rate = 1 mV/ms). C: averaged results (±SE) of persistent sodium currents from 11 caudal hypothalamic neurons during normoxia and hypoxia (ramp rate = 1 mV/ms, *P < 0.05).

The second method of analyzing the persistent current is demonstrated in Fig. 9. Long (60 ms) depolarizing steps from a holding potential of -90 mV were induced in a solution containing a normal sodium concentration and the current recorded during the last 10 ms of the step. Figure 9A shows the latter portion of the current response to the step depolarization that corresponds to the persistent sodium current; the initial fast-inactivating current in the initial part of the step has ceased by this point and has been omitted in the figure. The persistent sodium current was significantly increased from the control conditions, which returned to near control levels when normoxia was restored following hypoxia (Fig. 9A). Furthermore the inclusion of TTX (2 µM) into the bath completely attenuated the persistent current in these neurons as shown in Fig. 9A. The activation range of this persistent current was slightly more hyperpolarized than the activation range of the fast-inactivating current and hypoxia significantly enhanced the current throughout the entire activation range (Fig. 9B). The magnitude of this current was relatively small compared with the random noise, therefore activation curves were not obtained due to the low correlation coefficients with the Boltzman equation. When the peak persistent currents from all 10 neurons tested were compared, there was a significant enhancement in current at the end of the hypoxic period as compared with the end of the normoxic period (-93.3 ± 15.5 vs. -130.6 ± 25.5 pA, P < 0.05; Fig. 9C).



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Fig. 9. A: example of the increased persistent sodium current during hypoxia elicited by an elongated (60 ms) step depolarization in 1 caudal hypothalamic neuron. B: averaged persistent current responses (±SE) initiated by elongated depolarization steps during normoxia and hypoxia over the entire range of activation (-60 to -5 mV) from a holding potential of -90 mV. C: averaged peak persistent currents (±SE, n = 10) during normoxia and hypoxia in caudal hypothalamic neurons (*P < 0.05).


    DISCUSSION
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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
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The present study demonstrates that both the fast-inactivating and persistent sodium currents in rat caudal hypothalamic neurons are increased during a brief period of hypoxia. These findings significantly enhance our understanding of the mechanism of neuronal stimulation that occurs during hypoxia in these cells. The observed augmentation of the sodium currents during hypoxia may affect the neurons in this region in two ways. First, the increased amplitude and hyperpolarized activation curve values of the fast-inactivating sodium current observed during hypoxia can lead to a lower threshold for firing action potentials as well as a more robust firing response in the form of an increased frequency of spiking (Tombaugh and Somjen 1996; Werkman et al. 1997). Second, the increase in the persistent sodium current during hypoxia would likely cause a significant shift in the excitability potential of the cell due to the increase in membrane conductance from the inward sodium current flow (Crill 1996; Taylor 1993). Thus these changes in the sodium currents during hypoxia are most likely responsible for the increased firing frequency and depolarization observed in caudal hypothalamic neurons during hypoxia.

Neurons in the caudal hypothalamus have significant control over cardiovascular and respiratory function. Stimulation of this area leads to increases in respiratory rate, tidal volume, blood pressure, and heart rate (Waldrop and Porter 1995). Although this region is not absolutely essential for basic cardiorespiratory function, it is believed that the primary function of neurons in the caudal hypothalamus is to modulate cardiorespiratory function during various physiological conditions (Horn and Waldrop 1998). During periods of increased metabolic demand such as exercise and hypoxia, caudal hypothalamic neurons are activated and, via synapses on brain stem sympathetic and respiratory neurons, contribute to the increases in ventilation and perfusion necessary during these states (Horn and Waldrop 1997; Ryan and Waldrop 1995; Vertes and Crane 1996; Waldrop and Porter 1995; Waldrop et al. 1996).

Recent work has focused on the specific manner in which neurons in the caudal hypothalamus can react to changes in oxygen tension and subsequently modulate respiratory output. This work was started 35 yr ago in the rabbit with the demonstration of an increased firing frequency during hypoxia in caudal hypothalamic neurons (Cross and Silver 1963). More recent studies in the cat have shown that a significant proportion of neurons in the caudal hypothalamus increase their firing frequency during systemic hypoxia (Dillon and Waldrop 1993). In addition, the increase in firing rate during hypoxia was maintained following the abolition of peripheral chemoreceptor input. Similar results were found in the rat with the added finding that a large number of these cells display rhythms correlated to either the sympathetic or respiratory cycles and also sent projections to midbrain and medullary cardiorespiratory centers (Barman 1990; Ryan and Waldrop 1995). When studied in an isolated in vitro brain slice preparation, a large majority of rat caudal hypothalamic neurons are activated during hypoxia that persists even during complete synaptic blockade (Dillon and Waldrop 1992). Therefore these neurons appear to have the ability to directly respond to changes in oxygen tension and likely transduce this response into an alteration in physiological function via connections with the respiratory control centers in the brain stem.

When analyzed using voltage-clamp techniques, caudal hypothalamic neurons responded to hypoxia with a sustained inward current and increase in membrane conductance (Horn et al. 1999). This study showed that the inward current response is unaffected by calcium channel blockers but is almost completely abolished with the sodium channel blocker, tetrodotoxin. Furthermore the increased membrane conductance is greatly reduced when extracellular sodium is eliminated adding to the evidence of a sodium current mediating the membrane response to hypoxia in these cells.

The present work significantly broadens our understanding of how sodium currents are affected during hypoxia in caudal hypothalamic neurons. The amplitude of the fast-inactivating sodium current in these neurons was significantly increased during hypoxia. One explanation for this result is that oxygen deprivation directly causes a conformational change in the sodium channel that alters the conductance through this channel. Alterations due to hypoxia in the amplitude of fast-inactivating sodium current have been observed before in rat hippocampal and human neocortical neurons; however, the alteration observed in these studies was a decrease in current amplitude (Cummins et al. 1993; O'Reilly et al. 1997).

Because a wide variety of intracellular signaling molecules are known to modulate sodium channel activity in mammalian neurons, another possible mechanism of the change in current during hypoxia is a change in intracellular signaling molecules by the hypoxic stimulus (Catterall 1999; Ma et al. 1994; Smith and Goldin 1992, 1997). An example of this type of modulation was shown in hippocampal neurons where the hypoxic-induced decrease in sodium current amplitude was blocked by protein kinase C (PKC) inhibitors (O'Reilly et al. 1997). Because the amplitude of the fast-inactivating currents in caudal hypothalamic neurons was increased during hypoxia, stimulation of PKC is unlikely to be responsible for the alteration observed. In fact, one argument for the results observed in this study would be that PKC is decreased during hypoxia due to a decrease in aerobic metabolism that leads to a disinhibition of the fast-inactivating currents. This is unlikely, however, since several studies have shown that the activity of PKC is increased in neurons or neuronal-like cells by short periods of hypoxia or ischemia (Cardell and Wieloch 1993; Gozal et al. 1998; Kobayashi and Millhorn 1999; Wieloch et al. 1991, 1993).

Since hypoxia has been shown to decrease intracellular pH, the modulation of sodium current during hypoxia may be explained by an indirect effect of hydrogen ion concentration on channel function (Fujiwara et al. 1992). In fact, the biophysical properties of several ion channels and the activity of central neurons are known to be pH dependent (Chesler 1990; Dean et al. 1989; Lehmenkuhler et al. 1989; Liu et al. 1999). Intracellular acidification, however, has been shown to decrease the function of fast-inactivating sodium currents in rat hippocampal neurons, suggesting a different mechanism for sodium current enhancement during hypoxia in caudal hypothalamic neurons (Tombaugh and Somjen 1996). Therefore a plausible mechanism for the hypoxic increase in the amplitude of this current is a direct effect of a lack of oxygen on the channel, although more substantial evidence through the use of single-channel recordings is needed.

Taken in conjunction with the hyperpolarized shift in the activation curve for these neurons during hypoxia, the increase in fast-inactivating sodium current amplitude would most likely cause an enhancement of excitability. Since the main effects of hypoxia on caudal hypothalamic neurons are a depolarization and increased firing frequency, the modifications of the fast-inactivating sodium channels observed in the present study can partially explain these responses. The increase in amplitude of this sodium current indicates either a change in channel conductance or gating properties leading to an enhancement of excitability (Tombaugh and Somjen 1996). In addition, the hyperpolarized activation curve would lower the threshold for activation of these neurons during hypoxia leading to a greater ability to spike (Werkman et al. 1997). In contrast, the hyperpolarized inactivation curve during hypoxia could lead to a decrease in excitation because the inactivation persists longer into the repolarization phase and thus limits the ability to increase firing frequency (Werkman et al. 1997). However, the hyperpolarized inactivation curve could also allow the neuron to repolarize more quickly due to the quicker initiation of inactivation and thus lead to a shorter action potential duration and subsequent increase in firing frequency. To differentiate between these two possibilities, a detailed analysis of the effect of hypoxia on the time course of inactivation recovery is needed.

In addition to the fast-inactivating sodium current, the noninactivating or persistent sodium current was significantly enhanced by hypoxia in these neurons. The persistent sodium current can be either TTX-sensitive or resistant and is 1-3% the amplitude of the fast-inactivating current with similar voltage sensitivity (Bezanilla and Armstrong 1977; Chandler and Meves 1970; Cummins and Waxman 1997; Parri and Crunelli 1998). Single-channel analyses have added to the growing evidence that these currents are a variant in the gating kinetics of fast-inactivating sodium channels and not a distinct protein channel (Alzheimer et al. 1993; Keynes and Elinder 1998). One of the primary roles postulated for this current in central neurons is to increase the firing rate of neurons through a sustained sub-threshold current (Taylor 1993). This can occur with such small currents because at the more hyperpolarized level of activation in this current, the overall impedance of the cell is high thereby allowing small currents to cause depolarizations significant enough to reach the action potential threshold (Crill 1996). In addition, this current is important in sustaining rhythmic firing behavior in a number of neuronal types (Alonso and Llinas 1989; D'Angelo et al. 1998). Recent single-channel work has shown that the persistent sodium current in rat ventricular myocytes is enhanced during a short period of hypoxia, which is due to an increase in the open probability of the channel (Ju et al. 1996). Similar increases in the persistent sodium current during hypoxia were found in rostral ventrolateral medullary neurons in the rat (Kawai et al. 1999). In these cells, which are stimulated during hypoxia, both a decrease in molecular oxygen as well as treatment with cyanide had similar enhancing effects on the persistent sodium current. In contrast, cyanide caused a decrease in the persistent sodium current in rat CA1 hippocampal neurons, which primarily respond to hypoxia with a hyperpolarization (Hammarstrom and Gauge 1998). Thus the effect of hypoxia on this current directly correlates with the membrane response to hypoxia in several cell types.

In conclusion, a brief period of hypoxia causes an increase in the amplitude of the fast-inactivating and persistent sodium currents in acutely dissociated rat caudal hypothalamic neurons. Furthermore the activation and inactivation curves for the fast-inactivating sodium current were significantly hyperpolarized during hypoxia in these cells. These results suggest that sodium currents are primarily responsible for the depolarization and increased firing frequency response observed in these cells during hypoxia and at least in part comprise the cellular mechanism allowing these neurons to modulate respiratory function during hypoxia.


    ACKNOWLEDGMENTS

We thank Dr. Gabriel G. Haddad for advice on the acute neuronal dissociation protocol and Dr. Phillip Best for a critical review of the manuscript.

This work was supported by National Heart, Lung, and Blood Institute Grant HL-06296 and American Heart Association-Illinois Affiliate Grant-in-Aid to T. G. Waldrop and American Heart Association-Illinois Affiliate Grant 9704427A to E. M. Horn.

Present address of E. M. Horn: Barrow Neurological Institute, Division of Neurosurgery, 350 W. Thomas Ave., Phoenix, AZ 85013.


    FOOTNOTES

Address for reprint requests: T. G. Waldrop, Dept. of Molecular and Integrative Physiology, University of Illinois, 524 Burrill Hall, MC-114, 407 S. Goodwin Ave., Urbana, IL 61801 (E-mail: twaldrop{at}uiuc.edu).

Received 22 March 2000; accepted in final form 28 July 2000.


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