Division of Neuroscience and Structural and Computational Biology and Molecular Biophysics Program, Baylor College of Medicine, Houston, Texas 77030
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ABSTRACT |
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Ji, Daoyun and
John A. Dani.
Inhibition and Disinhibition of Pyramidal Neurons by Activation
of Nicotinic Receptors on Hippocampal Interneurons.
J. Neurophysiol. 83: 2682-2690, 2000.
Nicotinic
acetylcholine receptors (nAChRs) are expressed in the hippocampus, and
their functional roles are beginning to be delineated. The effect of
nAChR activation on the activity of both interneurons and pyramidal
neurons in the CA1 region was studied in rat hippocampal slices. In CA1
stratum radiatum with muscarinic receptors inhibited, local pressure
application of acetylcholine (ACh) elicited a nicotinic current in 82%
of the neurons. The majority of the ACh-induced currents were sensitive to methyllycaconitine, which is a specific inhibitor of 7-containing nAChRs. Methyllycaconitine-insensitive nicotinic currents also were
present as detected by a nonspecific nAChR inhibitor. The ACh-sensitive
neurons in the s. radiatum were identified as GABAergic interneurons by
their electrophysiological properties. Pressure application of ACh
induced firing of action potentials in ~70% of the interneurons. The
ACh-induced excitation of interneurons could induce either inhibition
or disinhibition of pyramidal neurons. The inhibition was recorded from
the pyramidal neuron as a burst of GABAergic synaptic activity. That
synaptic activity was sensitive to bicuculline, indicating that
GABAA receptors mediated the ACh-induced synaptic currents.
The disinhibition was recorded from the pyramidal neuron as a reduction
of spontaneous GABAergic synaptic activity when ACh was delivered onto
an interneuron. Both the inhibition and disinhibition were sensitive to
either methyllycaconitine or mecamylamine, indicating that activation
of nicotinic receptors on interneurons was necessary for the effects.
These results show that nAChRs are capable of regulating hippocampal
circuits by exciting interneurons and, subsequently, inhibiting or
disinhibiting pyramidal neurons.
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INTRODUCTION |
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Nicotinic acetylcholine receptors (nAChRs)
play roles in modulating cognitive functions, including learning and
memory (Levin and Simon 1998). Nicotine administration
facilitates learning and memory on many behavioral tasks in both young
and old mammals, and some of the improvements suggest the hippocampus
is one target for nicotinic modulation (Arendash et al.
1995
; Levin and Torry 1996
; Socci et al.
1995
).
The hippocampus is a center for learning and memory and is
especially important for spatial learning (Eichenbaum
1996; Wilson and McNaughton 1993
; Wood et
al. 1999
). The hippocampus receives extensive cholinergic
innervation from the medial septum-diagonal band complex (Alonso
and Amaral 1995
; Woolf 1991
; Yoshida and Oka 1995
), and there is strong expression of nAChRs in the
hippocampus (Martin and Aceto 1981
). In the mammalian
hippocampus,
7 and
2 subunits are widely distributed, but other
subunits also are present (Deneris et al. 1988
;
Rubboli et al. 1994
; Séguéla et al.
1993
; Wada et al. 1989
). In cell culture rat
hippocampal neurons express three types of nicotinic currents
(Alkondon and Albuquerque 1993
). The vast majority of
these neurons, however, display a rapidly activating and desensitizing
Type IA current, which is sensitive to
-bungarotoxin and
methyllycaconitine (MLA). The pharmacology indicates that the Type IA
current is mediated by
7-containing nAChRs (Alkondon et al.
1994
; Zarei et al. 1999
). That interpretation
was verified because Type IA currents are absent from hippocampal
neurons derived from
7-null mutant mice (Orr-Urtreger et al.
1997
). A more rare current with slower kinetics in hippocampal
cultures is called the Type II current, and it is inhibited by
dihydro-
-erythroidine or high concentrations of mecamylamine. Mutant
mice lacking
2 do not display Type II nAChR currents (Zoli et
al. 1998
).
Recent advances have begun to reveal the functional roles of nAChRs in
the hippocampus. The high calcium permeability of the 7-containing
nAChR (Castro and Albuquerque 1995
; Rathouz et
al. 1996
; Séguéla et al. 1993
)
enables it to enhance the release of both glutamate and GABA via
presynaptic or preterminal mechanisms in the hippocampus
(Alkondon et al. 1997
; Gray et al. 1996
;
Radcliffe and Dani 1998
; Radcliffe et al.
1999
). On the basis of these results, a predominantly
presynaptic role has been assigned to the hippocampal nicotinic
receptors. Recently, however, fast nicotinic synaptic transmission and
somatic and postsynaptic nicotinic responses have been discovered on
hippocampal interneurons (Alkondon et al. 1998
, 1999
;
Frazier et al. 1998a
,b
; Hefft et al.
1999
; Jones and Yakel 1997
; McQuiston and
Madison 1999
). These results suggest that postsynaptic nAChRs
might influence hippocampal circuits via GABAergic pathways. The aim of
the present study was to determine whether nicotinic activation of CA1
interneurons has the ability to produce inhibition or disinhibition of
pyramidal neurons. We found that exogenous activation of interneurons
by a nicotinic agonist can inhibit CA1 pyramidal neurons via
GABAA synaptic activity or disinhibit pyramidal
neurons by reducing spontaneous GABAA synaptic activity.
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METHODS |
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Slice preparation and electrophysiology
Sprague-Dawley rats (16-30 day old) were anesthetized with a mixture of ketamine (42.8 mg/ml), xylazine (8.6 mg/ml), and acepromazine (1.4 mg/ml) at a dosage of 0.05 ml/10g and were decapitated. Coronal or horizontal slices (300-400 µm thick) were cut in ice-cold cutting solution of the following composition (in mM): 220 sucrose, 2.5 KCl, 30 NaHCO3, 1.25 KH2PO4, 10 dextrose, 7 MgCl2, and 1 CaCl2, bubbled with 95% O2-5% CO2. Slices were transferred into a holding chamber, containing the external solution (in mM): 125 NaCl, 2.5 KCl, 25 NaHCO3, 1.25 KH2PO4, 25 dextrose, 1 MgCl2 and 2 CaCl2, oxygenated with 95% O2-5% CO2. After a 30-min recovery at 35°C, slices were maintained at room temperature and were used for recording in the following 5 h.
Neurons in CA1 stratum radiatum were recorded at 32-34°C, but CA1
pyramidal neurons were recorded at room temperature to reduce spontaneous GABAergic activity. In all of the experiments, 0.5-1 µM
atropine was added to the external solution to block muscarinic acetylcholine receptors. We use the term "block" rather than
"inhibition" in these circumstances to prevent confusing receptor
inhibition and GABAergic-mediated inhibitory synaptic currents. When
GABAergic currents were recorded from CA1 pyramidal neurons,
6-cyano-7-nitroquinoxaline-2,3-dione disodium (CNQX, 25 µM) and
(±12)-2-amino-5-phosphonovaleric acid (AP-5, 50 µM) were added to
block glutamatergic activity. To record from interneurons, the internal
solution in the recording pipettes contained the following (in mM): 115 K-gluconate (KGlu), 20 KCl, 10 N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic
acid, 10 ethylene glycol-bis (-aminoethyl ether) N,N,
N',N'-tetraacetic acid (EGTA), 4 ATP (magnesium salt), 0.3 GTP
(sodium salt), and 7 phosphocreatine, adjusted to pH 7.3-7.4 with KOH.
Normally the distribution of chloride makes GABAergic synaptic activity
difficult to detect when the holding potential of the voltage clamp is
near the resting potential because that holding potential is also near
the reversal potential for chloride. To make the GABAergic synaptic
currents in CA1 pyramidal cells easier to detect, 115 KGlu and 20 KCl
were replaced by 135 CsCl to raise the intracellular
Cl
while also improving the space clamp. Under
conditions of high intracellular Cl
, however,
GABAA activity could depolarize the cell and thus
activate voltage-dependent regenerative currents in unclamped distal
dendrites. To minimize this problem while keeping chloride relatively
high, in 18 experiments, 75 CsCH3SO3 and 60 CsCl
replaced 115 KGlu and 20 KCl and lidocaine N-ethyl bromide
was added to inhibit voltage-dependent sodium current. When antagonists
(MLA, mecamylamine, bicuculline, tetrodotoxin) were used, they were
applied via bath perfusion. The solution flowing rate was adjusted to
~3 ml/min.
The patch-clamp recording pipettes had resistances of 2-4 M
when filled with the internal solution. Data were acquired with an
Axopatch amplifier and stored on an hard drive. Series resistance and
input resistance were monitored by injecting a small negative voltage
or current step throughout the experiment. Series resistances were
usually in a range of 5-30 M
and were left uncompensated. Data were
discarded if series resistance or input resistance changed by
30%.
Recording sites and local delivery of agonists
Figure 1 shows the locations of the recorded neurons and the arrangement of the recording pipette (R) and the "puffer" pipette (P). By pressure injection, the puffer pipette locally delivered the agonist (ACh) to a desired location. We used two recording paradigms. As shown in Fig. 1A, neurons located in the CA1 s. radiatum 100-300 µm away from the pyramidal cell layer were recorded, and ACh was locally delivered onto their soma. Most of these neurons were identified as interneurons based on their electrical properties. Some recorded neurons in this paradigm were located near the border between s. radiatum and s. lacunosum-moleculare. In the second recording paradigm (Fig. 1B), ACh was applied locally to an interneuron while recording from a pyramidal neuron. ACh often was applied to several interneurons in the s. radiatum before we recorded a response from the pyramidal neuron that was whole cell clamped. In this paradigm, the interneuron was located 50-400 µm lateral and >100 µm vertical to the patch-clamped pyramidal neuron. The puffer usually pointed directly onto the single visualized interneuron, and the agonist was diluted and spread with time as it was washed away in the bath. The arrangement optimized activation of the interneuron's soma, but other local areas would experience a slower raise of a lower ACh concentration. The injection pipette was arranged to parallel the pyramidal cell layer to avoid ACh application directly onto or near the patch-clamped pyramidal neuron's soma. This application method is much faster and focal than other application methods because the puffer can be placed into the slice, very near the target, and brief pressure applications are used to produce the desired effect.
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A picospritzer (Parker Instrumentation) was used to control the pressure and duration of the puffs that deliver the agonist. When a short (5-20 ms) puff was used, the injection pipette was positioned ~10 µm away from the interneuron's cell body and a pressure of 10 psi was used. When a longer (50 ms to 1 s) puff was used, the injection pipette was positioned 50-80 µm away and a pressure of 5 psi was used. Control experiments were performed to examine potential artifacts due to the puffing system. When external solution containing no agonist was puffed onto the soma, there was no response under our recording conditions (n = 30). However, if the puffer pipette was too close to the recording pipette and a long puff was applied, it could influence the whole cell seal. In that case, a slow and irregular current was seen in 3 of 14 neurons. Neurons were rejected if a slow, irregular current was observed during the pressure application, and we did not position the puffer pipette close to the cell during long agonist applications.
Data analysis
All the values are presented as means ± SE. Positive and negative current steps were injected and voltage responses were recorded to characterize electrophysiological properties of the recorded neuron. Resting membrane potentials were estimated within 5 min after establishing the whole cell recording configuration. Current steps (300-ms duration) that were smaller than the threshold required to induce action potential were injected, and input resistances were calculated by dividing the steady-state voltage responses by the injected currents. Action potentials elicited at threshold were used to determine fast afterhyperpolarization-potentials (AHPs). Fast AHPs were calculated as the difference between the most negative potential immediately after an action potential and the threshold potential. Firing frequencies and slow AHPs were determined from the action potential train with the maximum number of action potentials that could be elicited by a 1-s current step (200-1,200 pA). Slow AHPs were calculated as the most negative potentials after the action potential train relative to resting potentials. Negative current steps (300 ms, 100-500 pA) were injected to determine sag ratios, which were calculated from the most negative membrane potential divided by the steady-state potential in response to the injected negative current.
The charge transfer in pyramidal neurons induced by GABAA receptor synaptic activity was calculated to quantitate the inhibition induced by ACh application (as in Fig. 4B). The charge was integrated through the whole current trace (2 s). The average net charge transferred by ACh-activated GABAA receptors was calculated as the difference between the average ACh-induced charge transfer and the average charge transfer without ACh application. The ACh application was taken to have produced a significant difference in the calculated charge transfer based on the Student's t-test (P < 0.05).
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RESULTS |
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The results are based on our recordings from 31 pyramidal neurons and 88 neurons in the s. radiatum of the CA1 region.
ACh-induced nicotinic currents from neurons in the CA1 s. radiatum
Of the 88 recorded neurons in s. radiatum, 72 (82%) displayed a
nicotinic response to a somatic pressure application of ACh (0.2-1 mM;
Fig. 2). As described in the following
text, the ACh-sensitive neurons were identified as GABAergic
interneurons. In response to a brief (5-20 ms) application of a high
concentration (1 mM) of ACh, a fast activating current, with a profile
similar to Fig. 2A, top, usually was recorded. When a longer
application (200 ms to 2 s) was used, 37 of 45 neurons displayed a
fast current with rapid desensitization, 4 neurons responded with a
slow current with relatively slower kinetics (Fig. 2A,
bottom), and 4 neurons showed a current with both the fast
component and the slow component. The kinetics of the fast current is
similar to the Type IA current, and the slow current is similar to the
Type II current in hippocampal cell culture (Alkondon and
Albuquerque 1993). Although at least two types of current were
recorded, the fast current was the predominant response to the ACh
application in the CA1 s. radiatum.
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To determine whether the nAChRs on CA1 interneurons contain 7
subunits, MLA sensitivity was tested with 23 neurons. The ACh-induced currents from 20 interneurons were blocked by bath application of 20 nM
MLA, and the currents recovered after a 35-min washout (Fig. 2A,
top). The block was fast and complete. In 2 of 23 interneurons, there were slower nicotinic currents that were completely blocked by 20 nM MLA. In 3 of the 23 neurons, however, there were slower nicotinic
currents that were not completely blocked by 20 nM MLA, but that
current was inhibited by 25 µM mecamylamine (MEC) (Fig. 2A,
bottom). Mecamylamine at that concentration is a nonspecific inhibitor of nAChRs. The result suggests that nAChR subunits other than
7 can contribute to the nicotinic currents; however, most nAChRs on
CA1 s. radiatum interneurons are MLA sensitive and contain the
7 subunit.
To examine the possible contribution from glutamate receptors to the
current response, the effect of CNQX and AP-5 was tested on five
neurons. Bath application of CNQX (25µM) and AP-5 (50µM) did not
significantly change the amplitudes of the ACh-induced currents (97 ±3% of control, P = 0.47, Student's
t-test, n = 5). Therefore the recorded
ACh-induced currents were not significantly contaminated by
glutamatergic currents arising from glutamate release induced by
presynaptic nAChRs (Radcliffe and Dani 1998).
ACh application caused action potentials to be fired in ~70% (50 of 72) of the identified interneurons. In many interneurons, the firing was strong and lasted for seconds in response to a 1-s application of ACh (0.2-1 mM). In the three neurons we tested, the ACh-induced action potential trains were inhibited by 20 nM MLA (Fig. 2B, n = 3). This result indicates that stimulation of nAChRs can elicit trains of action potentials in s. radiatum interneurons.
ACh-sensitive and -insensitive neurons in the CA1 s. radiatum displayed different electrophysiological properties
Although the majority of the neurons in CA1 s. radiatum responded
with a nicotinic current, there were 16 neurons (18%,
n = 88) that did not respond with detectable nicotinic
current when ACh was pressure-applied onto their somas. We compared the
electrophysiological properties of the ACh-sensitive and -insensitive
neurons. The voltage responses to current injections of a typical
ACh-sensitive and a typical ACh-insensitive neuron in the s. radiatum
are shown in Fig. 3. The
electrophysiological property of eight ACh-responding neurons and four
ACh-insensitive neurons are summarized in Table 1. The ACh-sensitive neurons had
significantly larger fast-AHP, higher firing frequency, and higher
input resistance. There was no significant difference in the resting
potential, the sag ratio, or the slow-AHP between the two cell types.
The electrophysiological characteristics displayed by the ACh-sensitive
neurons indicate they were GABAergic interneurons (Lacaille et
al. 1987; Schwartzkroin and Mathers 1978
). The
ACh-insensitive neurons, on the other hand, displayed much smaller
fast-AHP, lower firing frequency and lower input resistance. Therefore
the ACh sensitivity distinguishes two types of neurons in the CA1 s.
radiatum.
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ACh application onto interneurons can produce direct GABAA receptor-mediated inhibition of pyramidal neurons
We examined how the ACh-induced excitation of interneurons affects
pyramidal neurons using the recording paradigm shown in Fig.
1B. When ACh (0.2-1 mM) was applied onto an interneuron in the s. radiatum, strong inhibition could be recorded in nearby pyramidal neurons. Special care was taken to avoid ACh application directly onto or near the patch-clamped pyramidal neuron. A
cesium-based solution was used in the recording pipettes, and the
intracellular Cl concentration was raised to
135 or 60 mM to amplify the GABAA-mediated current. The same intensity of response by the GABAergic interneurons would be elicited regardless of the solution inside the pyramidal neuron, but we would not easily measure the effect with physiological solutions because the resting potential then would be near to the
Cl
reversal potential.
A total of 47 pairs of interneurons and pyramidal neurons were
examined. In 14 pairs (30%), application of ACh (0.5-1 s) onto the interneuron induced a burst of synaptic current in the pyramidal neuron in the presence of 25 µM CNQX and 50 µM AP-5 (Fig.
4A). Under our experimental
conditions, the synaptic currents were inward when the neurons were
clamped at 50 mV. The duration of the responses varied from seconds
to tens of seconds. The longer GABAergic activity may have arisen from
activation of more than one interneuron by the ACh application. The
ACh-induced synaptic currents were blocked reversibly by 10 µM
bicuculline in all of the five tested pairs (Fig. 4Ac),
indicating that the inward currents were mediated by
GABAA receptors.
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Summarized in Fig. 4B are the data from the pairs where the
pyramidal neurons responded significantly to the ACh puffed onto the
interneurons. If the pairs were connected, ACh application onto the
interneuron generated GABAergic synaptic activity in pyramidal neurons
as indicated by the large amount of charge transferred by the
GABAA receptors. The ACh-induced responses showed
large neuron to neuron variability possibly because of variations in the strength of the connection between the interneuron and the pyramidal neuron. The recorded ACh-induced responses were larger when
the pyramidal neurons were perfused by patch electrodes containing 135 mM Cl as compared with 60 mM
Cl
(Fig. 4B).
Nicotinic pharmacology of the ACh-induced GABAergic synaptic activity
MLA (20 nM) is a specific antagonist of 7-containing nAChR, and
at a concentration of 25 µM, mecamylamine is a nonspecific nAChR
antagonist. In three of four experiments, ACh-induced GABAergic synaptic currents recorded from pyramidal neurons were blocked by MLA
(Fig. 5A), suggesting that
most responses were initiated by
7-containing nAChRs. In one
experiment, however, MLA only partially blocked the ACh-induced
GABAergic response recorded from the pyramidal neuron (Fig.
5B). After the GABAergic response recovered from the MLA
blockade, it was abolished completely by the subsequent application of
mecamylamine (Fig. 5B). The latter experiment indicates that
both
7-containing and non-
7 nAChRs were activated and were
capable of contributing significantly to the ACh-induced GABAergic
activity.
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Pyramidal neuron responses arose directly from ACh-induced interneuron action potentials
We studied the TTX sensitivity of the ACh-induced GABAergic synaptic currents measured from pyramidal neurons. Bath application of 0.5 µM TTX blocked the GABAergic synaptic activity induced in pyramidal neurons by ACh application onto interneurons (Fig. 6, n = 3). The synaptic response recovered after washout of TTX for 30 min. The data indicate that action potentials arising from the interneurons were required for the ACh-induced effect. Under our experimental conditions, the results suggest that the GABAergic synaptic activity recorded from the pyramidal neurons arose from the direct excitation of GABAergic interneurons by nAChRs.
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ACh-induced interneuron activity can produce disinhibition of pyramidal neurons
During most of this study, we recorded from pyramidal neurons with low spontaneous GABAergic activity. Under those conditions, the most common effect of the ACh application onto interneurons was GABAA-mediated inhibition of the pyramidal neurons. However, we also recorded a disinhibition of one pyramidal neuron (Fig. 7) that displayed strong spontaneous GABAergic activity in the presence of CNQX and AP-5. A 1-s pressure application of ACh onto an interneuron potently reduced the spontaneous GABAergic activity. The spontaneous activity recovered from the ACh application in a few seconds. Bath application of 20 nM MLA partially blocked the ACh-induced reduction of spontaneous GABAergic activity. After MLA was removed from the bath and the effect recovered, subsequent application of 25 µM mecamylamine totally blocked the ACh-induced disinhibition. Finally, the spontaneous activity was completely abolished by 10 µM bicuculline, indicating that it was mediated by GABAA receptors. These results suggest that activation of nAChRs on s. radiatum interneurons also can disinhibit pyramidal neurons by reducing tonic GABAA receptor mediated inhibition.
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DISCUSSION |
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Nicotinic responses were recorded from interneurons located in the
CA1 s. radiatum. The majority of the currents evoked by ACh were MLA
sensitive, indicating that most of the nAChRs contain the 7 subunit.
In the s. radiatum of the CA1 region, the ACh-sensitive neurons were
GABAergic, and most of them fired action potentials in response to ACh
application. Localized ACh application onto interneurons produced
TTX-sensitive, GABAA receptor-mediated inhibition of pyramidal neurons. The ACh-induced inhibition was mostly mediated by
7-containing nAChRs and, to a lesser degree, by non-
7 nAChRs. Our
data also indicate that activation of
7-containing and non-
7 receptors could produce disinhibition of pyramidal neurons by suppressing tonic GABAA activity. Our
experimental conditions enabled us to observe the GABAergic activity as
an inward current. With lower internal chloride, the same number of
GABAA receptors would open to hold the cell near
the chloride reversal potential, which is near the resting potential.
In that case, however, the GABAA activity would
not be observed as the inward currents shown in Figs. 4-7. The results
of this work show that nAChR activity on interneurons has the capacity
to produce pronounced inhibition or disinhibition in pyramidal neurons.
Thus nicotinic receptors in the CA1 region have the ability to
influence the activity of hippocampal circuits.
ACh-sensitive and -insensitive neurons in CA1 s. radiatum
In all of our experiments, muscarinic receptors were inhibited by
atropine. ACh application induced nicotinic currents in the majority
(82%) of s. radiatum neurons (also see Alkondon et al. 1998,
1999
; Frazier et al. 1998a
,b
; Hefft et
al. 1999
; Jones and Yakel 1997
; McQuiston
and Madison 1999
). ACh-sensitive and -insensitive neurons in
CA1 s. radiatum displayed distinctive electrophysiological properties.
ACh-sensitive neurons displayed electrical properties indicative of
GABAergic interneurons: high firing frequency, large fast-AHP, and
high-input resistance (Lacaille et al. 1987
;
Schwartzkroin and Mathers 1978
). The ACh-insensitive neurons, on the other hand, had a low firing frequency, a small fast-AHP, and a lower input resistance. Most ACh-insensitive neurons electrophysiologically resemble a cell type referred to as s. radiatum
giant cells (Maccaferri and McBain 1996
). Similar to pyramidal neurons and different from other cell types in this region,
this cell type is capable of undergoing direct long-term potentiation
(LTP). Although most neurons in this region are identified as GABAergic
neurons by glutamic acid decarboxylase immunoreactivity (Ribak
et al. 1978
; Woodson et al. 1989
), we cannot
exclude the possibility that the ACh-insensitive neurons are a small
percentage of excitatory pyramidal neurons displaced into the nearby s. radiatum.
Subtypes of nicotinic receptors in the hippocampus
Our results suggest that the major subtype of nAChR on CA1 s.
radiatum interneurons are 127-containing receptors. Hybridization studies indicated that
127 and
2 are widely expressed and that other subunits also are present in the hippocampus (Rubboli et al. 1989; Wada et al. 1993). In cell cultures
from the rat hippocampus, fast Type I currents arise from
7-containing nAChRs (Alkondon and Albuquerque 1993
;
Alkondon et al. 1994
; Zarei et al. 1999
). Slower Type II and Type III currents were only recorded from a small
percentage of cultured neurons. Although still rare, slow currents were
more common in our slice study than in cell culture. In the slice
preparation, however, agonist applications are more variable and may
not always reveal the true kinetics, especially when the kinetics are
fast. Sensitivity to selective antagonists, such as MLA, is a better
method to determine the nAChR subtype. In our experiments, only 3 of 23 neurons showed a slow residual current after the MLA blockade. These
findings are consistent with other studies (Alkondon et al.
1999
; Frazier et al. 1998b
; McQuiston and
Madison 1999
). When pyramidal neurons were recorded and ACh was
applied to the interneurons, MLA-insensitive effects were observed in
two of five connected pairs.
Inhibition and disinhibition of pyramidal cells by nAChRs on interneurons
Interneurons in the s. radiatum impose powerful inhibition on
pyramidal neurons (Sik et al. 1995). There are several
types of interneurons in this region that play different roles in
hippocampal circuits (Freund and Buzsaki 1996
;
Miles et al. 1996
). Basket cells and axon-axonic cells
mainly innervate the soma, the proximal dendrites, and the axon initial
segments of principal neurons. Many interneurons in s. radiatum
innervate distal dendrites, whereas some interneurons only innervate
other interneurons. Because the majority of interneurons in this region
responded to ACh application, many different types of interneurons
could be activated by nAChRs. Thus the modulation of hippocampal
circuits by nAChRs could be complicated and varied. Our study
demonstrates that local application of ACh onto interneurons can cause
both inhibition and disinhibition of pyramidal neurons. The ACh-induced
inhibition and disinhibition were mediated by the direct excitation of
interneurons by mainly somatic nAChRs, but our recording paradigm could
not prevent the agonist from reaching some preterminal or presynaptic
nAChRs. The location of our agonist puffer very near the interneuron
soma and the TTX sensitivity of the effect argue that somal nAChRs were
the primary site of action in this study. Most probably, activating
nAChRs on those interneurons that directly innervate pyramidal neurons
causes the inhibition, and activating nAChRs on those interneurons that
innervate other interneurons causes the disinhibition. ACh activation
of interneurons that then inhibit other interneurons would release some
pyramidal neurons from some of their inhibitory inputs (i.e.,
disinhibition). Such an indirect excitation of pyramidal neurons is
supported by a study that shows nAChR activation can increase GABA
activity in some interneurons (see Alkondon et al.
1999
).
Roles of nAChRs in learning and memory
One possible role for nAChRs is to influence synaptic plasticity
in the hippocampus. LTP and long-term depression (LTD) are candidates
for the cellular mechanism of learning and memory. Postsynaptic
depolarization is required for certain forms of hippocampal LTP
(Larkman and Jack 1995; Magee and Johnston
1997
; Markram et al. 1997
), and depolarization
of the pyramidal neurons is strongly shaped by inhibition. In that way,
LTP and LTD are influenced by the GABAergic activity (Paulsen
and Moser 1998
; Steele and Mauk 1999
;
Wallenstein and Hasselmo 1997
), and our results show that nAChR activity can excite interneurons and inhibit or disinhibit pyramidal neurons. Therefore nAChRs have the capacity to influence LTP/LTD by activating interneurons and, thereby, decreasing or increasing the postsynaptic depolarization of the pyramidal neurons. This modulatory influence of nAChRs will depend on the strength, timing, and connectivity of the interneurons excited by nicotinic activity.
Another possible role for nAChRs is to affect the rhythmic activity in
the hippocampus. One prominent property of hippocampal circuits is the
production of different rhythmic oscillations. Theta rhythm (4-12 Hz)
and gamma activity (40-100 Hz) often occur during paradoxical sleep or
while awake rats explore their environments (Chrobak and Buzsaki
1998; Vanderwolf 1969
). These rhythms provide important windows for synaptic plasticity underlying learning (Huerta and Lisman 1993
, 1995
). It is also known that
hippocampal interneurons are important for pacing the rhythmic
oscillations, which are modulated by septal inputs (Csicsvari et
al. 1999
; Dragoi et al. 1999
; Freund and
Buzsaki 1996
; Stewart and Fox 1990
). Because nicotinic activity can directly excite interneurons (Alkondon et
al. 1998
; Frazier et al. 1998a
; Hefft et
al. 1999
) and exert inhibition or disinhibition on principal
neurons, nAChRs could influence the rhythmic activity of the
hippocampus. Although CA1 interneurons and pyramidal neurons may be
capable of producing intrinsic theta oscillation (Chapman and
Lacaille 1999
; Leung and Yim 1991
), they need to
be driven near the firing threshold to generate the oscillation. The
excitatory nAChRs on interneurons have the capacity to depolarize the
interneurons toward their firing threshold and to drive the pyramidal
neurons back toward their resting potential. In this way, nAChRs could
influence the rhythmic activity in the hippocampus and participate in
synaptic mechanisms underlying learning and memory.
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ACKNOWLEDGMENTS |
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This work was supported by National Institutes of Health Grants NS-21229, DA-09411, and DA-12661.
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FOOTNOTES |
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Address for reprint requests: J. A. Dani, Div. of Neuroscience, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030-3498.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Received 13 August 1999; accepted in final form 28 January 2000.
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REFERENCES |
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