School of Physiology and Pharmacology, University of New South Wales, Sydney, NSW 2052, Australia
![]() |
ABSTRACT |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Zachariah, M. K., G. T. Coleman, D. A. Mahns, H. Q. Zhang, and M. J. Rowe. Transmission Security for Single, Hair Follicle-Related Tactile Afferent Fibers and Their Target Cuneate Neurons in Cat. J. Neurophysiol. 86: 900-911, 2001. Transmission from single, identified hair follicle afferent (HFA) nerve fibers to their target neurons of the cuneate nucleus was examined in anesthetized cats by means of paired recording from individual cuneate neurons and from fine, intact fascicles of the lateral branch of the superficial radial nerve in which it is possible to identify and monitor the activity of each group II fiber. Selective activation of individual HFA fibers was achieved by means of focal vibrotactile skin stimulation. Forearm denervation precluded inputs from sources other than the monitored HFA sensory fiber. Transmission characteristics were analyzed for 21 HFA fiber-cuneate neuron pairs in which activity in the single HFA fiber of each pair reliably evoked spike output from the target neuron at a fixed latency. As the cuneate responses to each HFA impulse often consisted of 2 or 3 spikes, in particular at HFA input rates up to ~20 imp/s, the synaptic linkage displayed potent amplification and high-gain transmission, characteristics that were confirmed quantitatively in measures of transmission security and cuneate spike output measures. In response to vibrotactile stimuli, the tight phase locking in the responses of single HFA fibers was well retained in the cuneate responses for vibration frequencies up to ~200 Hz. On measures of vector strength, the phase locking declined across the synaptic linkage by no more than ~10% at frequencies up to 100 Hz. However, limitations on the impulse rates generated in both the HFA fibers their associated cuneate neurons meant that the impulse patterns could not directly signal information about the vibration frequency above 50-100 Hz. Although single HFA fibers are also known to have secure synaptic linkages with spinocervical tract neurons, it is probable that this linkage lacks the capacity of the HFA-cuneate synapse for conveying precise temporal information, in an impulse pattern code, about the frequency parameter of vibrotactile stimuli.
![]() |
INTRODUCTION |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Vibrotactile information from the
glabrous skin of the limb extremities is derived largely from
dynamically sensitive tactile afferent fibers associated with Pacinian
corpuscle (PC) receptors and from intradermal encapsulated receptors
that, in the cat, are known as Krause corpuscles. The two classes of
afferent fibers in the cat are known, respectively, as PC fibers and
rapidly adapting (RA) afferent fibers (Ferrington and
Rowe 1980a; Ferrington et al. 1984
; Iggo
and Ogawa 1977
; Jänig 1971
;
Jänig et al. 1968
). In the hairy skin, the
principal tactile afferent fibers responsible for vibrotactile
detection appear to be associated with hair follicles of the
guard hair type (Burgess et al. 1968
;
Merzenich and Harrington 1969
) as PC afferent fibers are
rare or absent in hairy skin (Brown and Iggo 1967
;
Tuckett et al. 1978
). The hair follicle
afferent (HFA) fibers are purely dynamically sensitive and
therefore display rapidly adapting responses to static skin
displacement. In response to vibrotactile stimuli they may respond to
frequencies up to 200-300 Hz but have substantially higher thresholds
than their glabrous skin counterparts (Merzenich and Harrington
1969
; Tuckett et al. 1978
). On the assumption
that human HFA fibers have similar thresholds to those in the cat,
these regional differences among tactile afferents from glabrous and
hairy skin may account, at least in part, for the higher subjective
thresholds for human vibrotactile sensation in hairy skin
(Merzenich and Harrington 1969
; Verrillo
1966
) compared with those in glabrous skin (Talbot et
al. 1968
).
However, whether the transmission characteristics across synaptic
junctions in the central tactile pathways also limit the capacity of
HFA fibers to contribute to subjective vibrotactile perceptual
performance is uncertain. While single HFA fibers are known to exert
potent synaptic actions on dorsal horn neurons that give rise to the
spinocervical tract (Brown et al. 1987a-c
), it is not
known whether they display a similar high security in their synaptic
linkage with target neurons of the dorsal column nuclei in the
principal tactile sensory pathway. The broad aims of the present study
were to examine the transmission characteristics for the synaptic
linkage formed between single HFA fibers and their target neurons of
the cuneate nucleus. Specific aims were to determine first whether
input from a single HFA fiber is sufficient to activate central target
neurons and therefore reveal whether the transmission of vibrotactile
information derived from individual HFA fibers can take place without
spatial summation; second, whether temporal summation is necessary for
the effective transmission of single HFA inputs to the cuneate neurons,
and third, whether vibrotactile frequency information, encoded in the
phaselocked impulse patterns of single HFA fibers, can be reliably
retained in a temporal patterning code in the course of transmission
across the cuneate synaptic linkage.
The analysis of transmission efficacy in the one-to-one linkage was
based on a simultaneous, paired recording paradigm of the type used in
our earlier studies of transmission security for PC and slowly
adapting tactile afferent fibers (Ferrington et al.
1987a,b
; Gynther et al. 1995
; Vickery et
al. 1994
). In the present study the simultaneous paired
recordings were made from an individual HFA fiber in an intact fascicle
of the lateral branch of the superficial radial nerve, and from a
cuneate target neuron of that identified HFA fiber. Preliminary reports
of this work have been presented in conference proceedings
(Zachariah et al. 1999a
,b
).
![]() |
METHODS |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Animal preparation and stimulation procedures
Data were obtained in experiments performed on adult cats in which anesthesia was induced and maintained with sodium pentobarbitone (40 mg/kg ip; <2 mg · kg-1 · h-1 iv; overdose at end of experiment). All experiments conformed with the Australian Code of Practice for the Care and Use of Animals for Scientific Purposes. The trachea was cannulated, as were the femoral artery and vein for the continuous monitoring of blood pressure and heart rate, and for infusion of anesthetic agent and fluids. Temperature was maintained at 38 ± 0.5°C. An extensive denervation of the forearm was performed by sectioning the ulnar, median, median cutaneous, and musculocutaneous nerves to preclude inputs arising from sources other than the nerve fascicles under study.
The lateral branch of the superficial radial nerve (LB-SRN) was exposed distal to the elbow and maintained under paraffin oil. In each experiment, longitudinal dissection of the nerve into approximately 10 fascicles was carried out over distances of 3-5 cm by opening the epineurial sheath, freeing individual fascicles from surrounding connective tissue within the overall nerve, and longitudinally dividing certain of the larger fascicles. The fascicles were initially kept in continuity with the CNS, to permit recording from individual fascicles by means of a platinum hook electrode. The innervation field of each could then be identified by manual stimulation of the dorsal surface of the forearm. Most fascicles were then sectioned leaving just three to five of them intact.
The skin in the field of superficial radial innervation on the dorsum
of the paw and the distal forelimb was shaved to allow accurate
positioning of the stimulator probe on hair follicles or at the root of
the hairs for effective stimulation of individual HFA fibers. The
receptive fields of individual fibers within the fascicles were
carefully mapped using low strength von Frey hairs (~0.02-0.2 g wt).
Three to five fascicles with identified and discrete innervation fields
on the paw dorsum were usually selected, and other fascicles, including
those that innervated the wrist or proximal forelimb or that had high
spontaneous activity, were sectioned, as was the medial branch of the
superficial radial nerve. Individual HFA fibers were selectively
activated by applying precisely controlled vibrotactile stimuli to
their receptive fields on the skin (Fig.
1) using a fine stimulus probe (0.25 mm
diam) driven by a feedback-controlled mechanical stimulator
(Ferrington et al. 1987a,b
; Vickery et al.
1994
; Zhang et al. 1996
).
|
The dorsal column nuclei (DCN) were exposed by removing small parts of the atlas and occiput. Single cuneate neurons, in the region between 1 and 4 mm caudal to the obex, that received input from an identified HFA fiber were located and recorded extracellularly by means of tungsten microelectrodes. When a cuneate neuron was activated by hair inputs from the dorsum of the distal forelimb, its receptive field was mapped with the aid of a dissecting microscope and von Frey hairs, and paired simultaneous recordings were then made from the neuron and from individual HFA fibers that exerted suprathreshold excitatory actions on this cuneate neuron. Selective activation of individual HFA fibers was achieved by means of focal tactile stimuli delivered to the skin at the point of maximum sensitivity for the HFA fiber, with the 0.25-mm-diam flat-tipped probe, usually oriented perpendicular to the skin surface and placed, in the resting position, fractionally above it (~100 µm). Vibrotactile stimuli were delivered in 1-s-long trains with the sinusoidal vibration superimposed on a 1.5-s background step indentation of 400 µm, and commenced 300 ms after the onset of the step (Fig. 1, inset). Stimulus repetition rate was usually 1 per 8-10 s to allow recovery of skin position.
Recording and analysis procedures
Impulse activity recorded from the afferent fiber-cuneate neuron
pair was stored on magnetic tape and in a laboratory computer. Impulse
rates (in imp/s) were obtained in response to the 1-s vibration trains
to plot stimulus-response relations for the HFA fiber-cuneate neuron
pairs. Transmission characteristics for the pair were also quantified
with two further measures, the transmission security, and a
cuneate spike output measure. The first, transmission security, was defined as the proportion of HFA impulses that evoked a
response in the cuneate neuron. This spike output measure was calculated from latency histograms (LHs) (Vickery et al.
1994), which are modified cross-correlograms constructed from
impulse activity elicited in the cuneate neuron in response to HFA
fiber impulses, by treating successively each HFA fiber impulse as
time 0. The LHs permitted accurate determination of the
latency to the onset of the central neuron response, as well as the
transmission security measure. The second measure, of cuneate spike
output to successive HFA input spikes, was obtained when the HFA input fiber was responding (at least in the early segment of the vibration train) at or near a regular 1:1 impulse level; that is, with one impulse discharging on each cycle of the vibration. This spike output
measure was computed as a mean from usually five successive response
sequences for a given vibration stimulus.
Evaluation of temporal patterning in HFA-cuneate paired responses to vibration
The analysis of temporal patterning in the vibrotactile
responses was based on construction of peristimulus time histograms (PSTHs) and cycle histograms from which quantitative measures of phase
locking could be obtained (Ferrington et al. 1987a,b
; Mackie et al. 1998
, 1999
; Vickery
et al. 1994
). The cycle histograms use a pulse associated with
the onset of each successive cycle in a vibration train as a stimulus
marker and display the incidence of impulse occurrences at different
times throughout the period of the vibration cycle. Two measures of
phase locking were obtained, the percentage entrainment and
the resultant. Percentage entrainment corresponded to the
maximum percentage of total impulse occurrences that falls within any
continuous half cycle of the vibration cycle period and could range
from a minimum of 50%, characteristic of a rectangular distribution in
the cycle histogram and an absence of phase locking, to a maximum of
100%. The resultant (R) was derived from the cycle histogram
distributions as a measure of vector strength in the cyclic
distribution. This measure is based on directional or angular
statistics (Mardia 1972
) and quantifies the phase
coherence or synchronization in the impulse distribution. This measure
has been used in evaluating the entrainment of auditory responses to
tonal stimuli (e.g., Bledsoe et al. 1982
; Lavine 1971
) and in the somatosensory system for quantifying the
entrainment of responses to vibrotactile stimuli (Greenstein et
al. 1987
; Mackie et al. 1998
; Vickery et
al. 1994
). The resultant, R, was calculated from
each cycle histogram distribution according to the formula
![]() |
![]() |
RESULTS |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Input to cuneate neurons from single HFA fibers
The innervation field on the dorsal surface of the forearm was mapped for each of the fascicles of the LB-SRN that had been separated by longitudinal division of the nerve. This was carried out by recording from each fascicle in turn with a platinum hook electrode while manually stimulating the skin with a fine brush or von Frey hair. Most fascicles were then sectioned leaving usually three, but up to five, in continuity with the CNS. Fascicles that were selected to remain intact were chosen according to the following criteria. First, the fascicle needed to be sufficiently fine that the signal-to-noise ratio (usually 5-10:1) for single fiber responses ensured a clear discontinuity between the recorded signals and background noise levels (see Figs. 1, 2, and 4). Second, low levels of spontaneous activity were required to permit clear isolation of activity from the identified HFA fiber under study. Third, each of the selected fascicles had a discrete field of innervation, on the forearm, precluding the risk of contaminating inputs being generated over fascicles other than the one being monitored. Other nerves to the forearm were sectioned, including those that have some overlap with the innervation field of the LB-SRN, such as the medial branch of superficial radial and the ulnar nerve. We can therefore be quite confident in our assumption that, in the paired recording analysis, the monitored HFA fiber was the only one responsible for the observed cuneate responses.
Location of cuneate neurons activated by single HFA fibers
Transmission characteristics were examined for 21 HFA
fiber-cuneate neuron pairs in which activity in single, identified HFA fibers reliably evoked spike output from the target neuron (e.g., Fig.
1, inset). The cuneate neurons were identified by
microelectrode tracking in the region of distal forelimb representation
of the nucleus while lightly brushing the skin in the innervation zones of the selected, surviving fascicles of the LR-SRN. All but 2 of the 21 recorded cuneate neurons were located between 1 and 3 mm caudal to the
obex, at a mediolateral position between 1 and 2 mm lateral to the
midline, and at depths below the brain stem surface of 0.5-3 mm. The
two neurons outside these locations included one found 0.5 mm caudal to
the obex and one at 0.8 mm lateral to the midline. These locations were
therefore consistent with the neurons being in the cluster zone of the
cuneate nucleus (Hand and Van Winkle 1977), where almost
all neurons sampled with the extracellular recording technique are
known to be thalamocortical projection neurons (Andersen et al.
1964
; Brown et al. 1974
; Gordon and Jukes
1964
).
Capacity of single HFA fibers to drive cuneate neurons
Our interpretation that the monitored HFA fiber was uniquely responsible for the associated cuneate neuron responses in circumstances such as the Figs. 1 and 2 traces, is based, as indicated above, on the denervation procedures and the stated signal-to-noise recording requirements. However, in addition, it is apparent in these figures that the cuneate neuron responses were dependent on the prior occurrence of the observed HFA fiber spike and followed at a consistent latency. Furthermore, when the HFA fiber failed to respond on some cycles of the vibrotactile stimulus in the impulse traces of Figs. 1 and 2, there was a correlated failure of response in the cuneate neuron. This is indicated by the asterisks in Fig. 1 and occurs in Fig. 2 on many of the 20-Hz vibration cycles at the low amplitudes (20 and 40 µm) and on two of the cycles at the 80-µm amplitude. Further strong evidence for the dependence of the cuneate spikes on the monitored HFA fiber spikes may be seen in the 80-µm paired traces in Fig. 2 when the HFA fiber displayed an unusual jump to a different phase relation with the vibration waveform on cycles 14, 17, 19, and 20 (as indicated by the asterisks). The related cuneate neuron response shows a corresponding shift in its phase relation.
|
The reliable correlation between the HFA spikes and the paired cuneate
responses is apparent from the connecting dot sequence in the impulse
traces of Figs. 1 and 2. The latency for the cuneate responses,
measured as the delay between the time of occurrence of the HFA spike
at the forearm recording site and the cuneate response ranged from 4.1 to 7.8 ms with a mean latency of 5.39 ± 1.25 ms (mean ± SD,
n = 20). This time corresponds to the conduction time
plus the time for synaptic transmission. If 1 ms is allowed for the
central synaptic delay time in the cuneate nucleus, one obtains an
average conduction velocity estimate of ~57 m/s, based on a
conduction distance of ~25 cm. This value is consistent with HFA
fibers falling in the group II range of afferent conduction velocities
even though the estimate includes both peripheral and the somewhat
slower central conduction components (Brown 1968). The
latency histograms (see METHODS) in Fig.
3 show the reliable, fixed latency of
cuneate responses for two HFA fiber-cuneate neuron pairs and were
constructed in both A and B from the cuneate
responses to ~200 input spikes in the HFA fiber's response to
vibration (20 Hz in A, and 30 Hz in B). The
paired impulse traces show that the double or multiple peaks in the
latency histograms are attributable to pairs or bursts of spikes
discharged by the cuneate neuron and do not reflect alternative
preferred phases of response for the cuneate neuron. The initial spike
in the cuneate response occurs with a fixed latency and shows less
temporal jitter than the second or third spike in the cuneate response.
|
Despite the close temporal correlation between the paired peripheral
and central responses, it was clear that the latter were not obtained
from the central axon of the peripherally recorded HFA fiber as they
often consisted of pairs or a burst of spikes and therefore did not
match the peripheral activity. In addition, at higher rates of HFA
fiber activity, the central response did not follow every occurrence of
the HFA spike, reflecting a breakdown of security for the synaptic
linkage. Furthermore, the central spikes had the di- or tri-phasic
configuration and duration 0.5 ms that is consistent with
extracellular microelectrode recording from the soma rather than an
axon (Winter 1965
).
Security of the synaptic linkage for HFA fiber-cuneate neuron pairs
Some variation was observed in the security of the linkage among the 21 HFA fiber-cuneate neuron pairs studied, although all 21 demonstrated the capacity of individual HFA fibers to exert suprathreshold excitatory actions on cuneate target neurons. Furthermore, as these actions entailed a single HFA spike generating spike output from the cuneate target neuron, it is clear that each of these HFA fibers generated unitary excitatory postsynaptic potentials (EPSPs) that exceeded threshold for spike discharge in the cuneate neuron. The high security of the synaptic linkage for many of the pairs was demonstrated by the presence of a cuneate neuron spike response to all or almost all of HFA spikes (Figs. 1 and 2). In Fig. 2, this capacity of the HFA fiber (1° in bottom trace of each pair) to securely drive the target neuron (2° in top trace) was sustained over the 1-s duration of the vibrotactile stimulus train as the discharge rate in the HFA fiber progressively increased as a function of vibration amplitude (from 20 to 100 µm) to a plateau level of 1 impulse per cycle of the 20-Hz vibration. At this 1:1 level of response, the HFA fiber unfailingly generated spike output in the cuneate neuron as indicated by the linking dotted lines in the lowest set of paired traces (100 µm) in Fig. 2.
Amplification of input in the HFA-cuneate neuron synaptic linkage
At the level of peripheral drive (20 imp/s) illustrated in Fig. 2, the majority of HFA spikes gave rise to a pair of spikes in the target neuron. Although not easily visualized at the illustrated time scale of the traces in Fig. 2, this was the case for each of the 1st 10 cycles of the 20-Hz input in the 100-µm trace and for 1/2 the remaining 10 cycles. However, the capacity for amplification of the input signal across the one-to-one HFA-cuneate linkage is clearer in the data shown for the two pairs illustrated in Fig. 3 and for the paired data in Fig. 4. This form of amplification of the input signal across the 1:1 synaptic linkage was apparent for half (10 of 21) of the HFA fiber-cuneate neuron pairs analyzed and consisted of a pair (Fig. 3A) or burst of spikes (Fig. 3B) in response to the single incoming HFA spike. In Fig. 4, a burst of three spikes was discharged by the cuneate neuron in response to the initial HFA spike in the incoming train at 20, 50, and 100 Hz, and an amplified response, in the form of a spike doublet, was sustained for subsequent HFA spikes in the 10-cycle segments shown in Fig. 4 at 20 and 50 Hz. However, the capacity of the linkage to sustain this amplification declined at higher rates of peripheral drive as illustrated in the lowest paired traces in Fig. 4 where the HFA fiber was driven at a rate of 100 imp/s by a 100-Hz vibrotactile stimulus. Nevertheless, each HFA spike activated on the 1st 8 cycles of the 100-Hz vibration stimulus elicits a reliable single spike output from the cuneate neuron.
|
For many pairs, the amplification of input signal was sustained over the vibrotactile response range of the HFA fiber, in particular, when the fibers were restricted in their vibrotactile responsiveness to impulse rates of <50 imp/s. The four stimulus-response relations of Fig. 5 show this amplification for one pair when the HFA fiber was activated by 20-, 50-, 80-, and 100-Hz vibration stimuli. The HFA fiber displayed a 1:1 response plateau of ~20 imp/s in response to the 20-Hz vibration and at other vibration frequencies attained a maximum impulse rate of 30-35 imp/s. However, at all levels of response in the presynaptic element, the cuneate neuron output was amplified reflecting the high-gain transmission for the HFA-cuneate synaptic linkage.
|
Quantification of transmission characteristics for HFA-cuneate neuron pairs
The transmission characteristics for HFA-cuneate pairs was
quantified in two ways (see METHODS). The first measure,
the transmission security, obtained from the latency histogram
distributions (Fig. 3), was defined as the proportion of HFA fiber
impulses that generated a response (1 or more impulses) in the cuneate
neuron. In most pairs whose records are illustrated in Figs. 1, 2, 4,
and 5, the transmission security was at or near 100%, while in Fig. 3,
A and B, the values were 81 and 96%,
respectively. For six pairs, in which the HFA fiber could be activated
by 20- or 30-Hz vibration to discharge, with a regular 1:1 pattern of
response over the full 1 s of the vibration train, the mean (±SD)
transmission security was 90 ± 13% (n = 6). This
value is similar to those obtained previously for the linkage between
individual SAI or SAII fibers and their target cuneate neurons
(Gynther et al. 1995; Vickery et al.
1994
). However, at higher vibration frequencies (
50 Hz), it
was not possible to obtain reliable pooled transmission security measures, as there were too few HFA fibers that could be reliably driven with a regular 1:1 pattern of response to vibration over the
full 1-s stimulus train.
A second quantitative measure of the transmission characteristics, the spike output from the cuneate neuron in response to successive HFA input spikes, is plotted in Fig. 6 as a function of vibration cycle number (or, in effect, input spike number, as the HFA fiber was responding at a 1:1 level over this early segment of the vibration train). For these four vibration frequencies, 10, 20, 50, and 100 Hz, the mean spike output for the cuneate neurons sampled was highest in response to the first and second incoming HFA spikes where the values were ~1.5-3.0 but remained high (>1.5) throughout the first 10 cycles at 10-50 Hz, reflecting the potent capacity for amplification in the linkage. However, at the 100-Hz level of drive, the amplification was not sustained beyond the first two HFA spikes, and mean spike output fell to levels between 0.5 and 1.0 spikes per cycle. The different mean spike output levels to the first spike (vibration cycle 1 on the abscissa) at the four frequencies reflect the different sample sizes and lack of identity in sampled pairs for the four vibration frequencies. The sample sizes were 3 at 10 Hz, 9 at 20 Hz, 8 at 50 Hz, and 3 at 100 Hz.
|
Although there was an initial decrement in the response output from the cuneate neuron over the first few cycles of the vibration (Fig. 6), the PSTHs on the left in Fig. 7, constructed over the full 1-s duration of the vibration train, show that cuneate responsiveness is then well maintained over the whole 1-s segment. Furthermore, part of the initial response decrement at the highest vibration frequency, 200 Hz, is attributable to a decline in the capacity of the HFA fiber to sustain its response to high-frequency vibration rather than a failure of the synaptic linkage.
|
Retention of phase locking and temporal patterning in responses to vibrotactile stimuli across the synaptic linkage between single HFA fibers and cuneate neurons
The expanded time scale for the PSTHs on the right-hand side in Fig. 7 shows the distribution of impulse occurrences over the 1st 30 cycles of vibration (at 30-200 Hz) for the cuneate neuron (top histogram of the pair) and the HFA fiber (bottom histogram). In both pre- and postsynaptic elements the responses are phase locked as reflected in the preferential grouping of impulses at intervals approximating the cycle period, in particular at 30, 50, and 100 Hz. At 200 Hz there is only a weak response from the cuneate neuron, but the fiber itself is only weakly activated, although it displays some preferential grouping of spikes in the first 50 ms of the vibration train. In the responses to the 30, 50, and 100 Hz stimuli, the phase locking appears little if at all degraded in the cuneate neuron response compared with that of the HFA fiber. However, quantitative comparison of the tightness of phase locking in the pre- and postsynaptic elements was undertaken by constructing paired cycle histograms, as illustrated in Fig. 8, A and B, from the responses of two representative HFA-cuneate pairs to vibrotactile stimulation at four frequencies (20-200 Hz in A, and 30-200 Hz in B). In both pairs the responses display tight phase locking in both the pre- and postsynaptic elements. In A, the HFA responses at 20 and 50 Hz are confined within about one-quarter of the cycle period and spread to about one-third at 100 Hz and one-half at 200 Hz, with percentage entrainment values of ~100% at all four frequencies and R values of >0.9 except at 200 Hz, where there is greater spread of impulse occurrences and an R value of 0.83. The target cuneate neuron showed a very similar tight phase locking in its response, although percentage entrainment and R values were slightly lower on account of some scattered spike occurrences reflecting a low level of spontaneous activity that was unrelated to the vibration-induced response. For the second pair in B, there was no quantitative difference in the phase locking of pre- and postsynaptic elements except for the slightly greater dispersion in the cuneate neuron responses at the highest frequency, 200 Hz, where the R value was 0.73 compared with 0.85 for the HFA fiber.
|
For six pairs that were studied at a series of frequencies, the quantitative measures of phase locking based on the R values are plotted as a function of vibration frequency in Fig. 9. The data for each linked pair in A and B show that, for all six, the cuneate neuron displays little decline in the phase locking of its responses compared with its HFA input fiber. On average the decline in R values over the vibration frequency range up to 100 Hz was <10% (from ~0.85 to ~0.8), although at the higher frequency of 200 Hz the central neurons showed a more marked decline, to an R value of ~0.45 compared with ~0.7 in the HFA fibers. Comparison of the peripheral R values with those of their central target neurons in Fig. 9C, using an ANOVA, confirmed that the small decline in the tightness of phase locking across this one-to-one synaptic linkage was significant (F ratio 4.08; P < 0.05). Furthermore, comparison, with a paired t-test, of the pooled peripheral R values in Fig. 9C with the pooled cuneate values, also confirmed the significant difference between the two samples (P < 0.001).
|
![]() |
DISCUSSION |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Identification of HFA-related cuneate neurons
The present study establishes that single HFA fibers have
the capacity to drive central neurons of the cuneate nucleus. These fibers, representing one of the major classes of tactile sensory nerve
fibers in the hairy skin, are readily distinguished on both functional
properties and receptive field characteristics from the slowly adapting
(SA) type I and II classes of tactile afferent that also innervate the
hairy skin. First, the HFA fibers display a pure dynamic sensitivity to
tactile stimuli, whereas the SAI and SAII afferents display maintained
responses to static skin displacements (Chambers et al.
1972; Gynther et al. 1992
, 1995
; Iggo and Muir 1969
; Vickery et al. 1992
,
1994
). Furthermore, the tactile sensitivity of SAI
afferent fibers is associated with the small epidermal specializations,
the ~200-µm-diam touch domes that are visible under the dissecting
microscope in the shaved hairy skin (Iggo and Muir
1969
), while that of SAII fibers is characterized by a
responsiveness to static as well as dynamic components of skin stretch
(Chambers et al. 1972
; Gynther et al. 1992
).
As the cuneate neurons driven by single HFA fibers had rostrocaudal,
mediolateral, and depth locations consistent with the cluster zone of
the nucleus (Hand and Van Winkle 1977), it is probable
that they were cuneo-thalamic projection neurons as most neurons
sampled electrophysiologically in this region can be activated antidromically from the contralateral thalamus (Andersen et al. 1964
; Gordon and Jukes 1964
). As the central
recording was concentrated in this region of the nucleus, we cannot
exclude the possibility that HFAs may also form potent connections with
cuneate neurons outside the cluster zone. Although our analysis does
not reveal the proportion of HFA fibers that have the capacity to drive
cuneate neurons, the isolation of 21 pairs in which single HFA fibers were capable of activating cuneate neurons suggests that this potent
form of synaptic linkage for the HFA fibers cannot be rare.
Security of the HFA-cuneate synaptic linkage
Previous studies have shown that the HFA class of tactile afferent
fibers project via the dorsal columns to the cuneate and gracile
divisions of the dorsal column nuclei and that HFA information is
represented in the lemniscal output from the nuclei (Brown et
al. 1974; Golovchinsky 1980
; Gordon and
Jukes 1964
). However, the present study provides the first
direct evidence that single HFA fibers can exert potent excitatory
actions on their cuneate target neurons, and that the minimum sensory
input conveyed over one of these sensory axons, a single impulse, can
generate spike output from cuneate neurons. These observations on the
21 pairs therefore establish indirectly that unitary EPSPs generated by individual HFA fibers in their cuneate target neurons may be
suprathreshold. Furthermore, the paired analysis demonstrates that
substantial amplification of the HFA input signal may occur, as the
individual HFA spikes often give rise to a closely spaced burst of
impulses consisting of either a spike doublet (Figs. 2, 3A,
and 4) or multiple spike burst (Fig. 3B) from the cuneate
neuron. This amplification could be sustained for perhaps the first
10-20 impulses arriving over the HFA fiber (Figs. 2, 4, and 6), in
particular at rates up to 50 imp/s of HFA input, and may serve to
enhance the probability of detecting weak tactile input signals.
Furthermore, the spike bursts constituting the amplified response
across this first synaptic junction in the dorsal column-lemniscal
pathway may provide for a form of temporal summation to ensure secure
transmission at the next synaptic junction, in the ventralposterior
thalamic nucleus. These properties for the linkage between single HFA
fibers and cuneate neurons suggest that transmission is optimized for
the detection of minimal tactile sensory inputs arising in the HFA input channels.
We have not investigated in this study the extent of divergent and
convergent actions on cuneate neurons by HFA afferents. However, as our
earlier paired recording study of the transmission characteristics for
individual PC-related afferent fibers revealed that individual PC
fibers could activate multiple target neurons (Ferrington et al.
1986), we might expect that this form of amplification may also
operate for HFA afferent fibers.
Vibrotactile signaling across the synaptic linkage between single HFA fibers and cuneate neurons
In the vibrotactile-induced responses of the HFA fiber-cuneate
neuron pairs, it is also clear that this linkage is able to retain a
temporally patterned sequence of spike activity in the cuneate neuron
that reflects the periodicity of the vibration stimulus. This is
apparent in the paired impulse trace records of Fig. 2, at 20 Hz, and
in Figs. 4 and 7 at frequencies up to 100 Hz. Furthermore, even when
the cuneate neuron was unable to respond on every cycle of the
vibration stimulus the impulse activity remains phase locked at
frequencies up to at least 200 Hz (Fig. 9). Although the tightness of
phase locking, quantified in terms of the vector strength or
R value calculated from the cycle histogram distributions,
shows a statistically significant decline in the process of synaptic
transmission from HFA fiber to cuneate neuron (Fig. 9C) this
decline was small, and no more than about 10% at vibration frequencies
up to 100 Hz. As the neural code for the frequency parameter of
vibrotactile stimuli appears to depend on an impulse patterning that
reflects the vibration periodicity (Ferrington and Rowe
1980b; Mountcastle et al. 1969
; Talbot et al. 1968
), it appears from paired HFA-cuneate response traces (Figs. 2 and 4) that in the process of synaptic transmission the cuneate neuron retains reliable information about the frequency parameter even in response to a selective input from a single HFA
sensory fiber. At least this appears to be the case for the single
HFA-cuneate linkage at vibration frequencies up to ~100 Hz. However,
the vibration-induced impulse rates generated in the cuneate neurons by
single HFA fibers were usually
100 imp/s, which are somewhat lower
than those generated by vibration when the input is carried by single
PC fibers (Ferrington et al. 1987a
) or single SAI or
SAII fibers (Gynther et al. 1995
; Vickery et al.
1994
). This difference appears to be attributable, in large part, to the more limited vibratory responsiveness of the HFA fibers
themselves compared with the PC, SAI, and SAII fibers, each of which
may be driven by focal vibration to rates of several hundred
impulses/second (Ferrington et al. 1987a
; Gynther
et al. 1995
; Vickery et al. 1994
). Thus any
limitation on the signaling of vibrotactile information by cuneate
neurons activated from hairy skin may reflect limitations in the
peripheral vibrotactile sensitivity of the HFA endings rather than
deficiencies in transmission efficacy at the HFA-cuneate synaptic
linkage. These constraints on peripheral HFA sensitivity probably
account for the higher subjective vibrotactile thresholds in hairy skin
compared with those in glabrous skin (Merzenich and Harrington
1969
; Talbot et al. 1968
; Verrillo
1966
) and may also mean that the capacity for vibrotactile
frequency discrimination in the hairy skin is poorer than in the
glabrous skin. However, actual Weber fraction (
f/f) data for this discrimination task are
available only for glabrous skin (Goff 1967
) or the
sparsely haired skin of the anterior forearm (Rothenburg et al.
1977
).
High transmission security at the dorsal column nuclei and the capacity of single tactile afferent fibers to contribute to perceptual experience
The high synaptic security revealed in the present study for the
linkage between single HFA afferent fibers and cuneate neurons is
similar to that shown in our previous studies for individual tactile
afferent fibers of the PC, SAI, and SAII classes (Ferrington et
al. 1987a,b
; Gynther et al. 1995
; Vickery
et al. 1994
). For all four classes, single fiber inputs are
able to drive central neurons of the dorsal column nuclei with
amplification of the input and reliable retention of temporal precision
in the transmission of information about dynamic vibrotactile stimuli.
As the HFA afferent fibers have now been shown to share these
transmission attributes, it appears increasingly certain that high
transmission security is a general attribute for different tactile
sensory nerve classes at the dorsal column nuclei. To generate spike
output from the postsynaptic neurons of this relay, there is no
dependence on the elaborate spatial or temporal integrative processing
that is required at the group Ia-motoneuron synaptic linkage. Instead, the transmission characteristics for tactile inputs at the dorsal column nuclei appear optimally designed for a sensing system, as
reflected in its capacity to transmit signals about tactile perturbations that generate the minimal sensory input, a single afferent action potential.
Despite the high security of HFA fiber synaptic linkages with cuneate
neurons, it remains uncertain whether the activation of individual HFA
fibers can generate a tactile percept as this class of tactile fiber
has not been tested for this capacity with the intraneural
microstimulation procedure. When this microneurography technique has
been used to selectively activate single tactile afferent fibers of
other classes in conscious human subjects, it has revealed marked
differences among these classes (Macefield et al. 1990;
Ochoa and Torebjörk 1983
; Vallbo et al.
1984
). For example, the activation of single PC and RA fibers,
and most SAI fibers, elicits a perceptual response, but the activation
of single SAII fibers fails to do so. Furthermore, the activation of
single SAI fibers with a train of electrical stimuli at 20-100 Hz
elicits a subjective sense of steady skin pressure, but fails to
generate any flutter-vibration percept in contrast to PC and RA fibers when activated in this way. However, despite these limitations for SAI
and SAII fibers, the paired simultaneous recording analysis in the cat
of transmission characteristics reveals a security and temporal
precision of signaling for single SAI and SAII fibers that matches that
for single PC afferent fibers (Ferrington et al.
1987a
,b
; Gynther et al. 1995
; Vickery et
al. 1994
). Our original hypothesis, that the differential
capacity of these fiber classes to contribute to perception when
activated singly with the microneurography procedure, may be
attributable to differential transmission characteristics at the dorsal
column nuclei is therefore not born out by the results. Thus the
explanation (unless there were some marked species difference between
cat and man in this respect) must presumably be at higher levels of the
sensory pathway, or be attributable to a more restricted central
divergence and therefore a failure of individual SAI or SAII fibers to
activate the necessary critical mass of central neurons for
perceptual recognition. In view of these considerations, the high
security of synaptic transmission for HFA fibers at the cuneate nucleus
cannot be taken as evidence of their capacity for generating a
perceptual response if activated singly.
High security for HFA linkages in parallel ascending sensory pathways
The HFA fibers, like most group II tactile afferent classes,
project not just to the dorsal column nuclei but also to the dorsal
horn, where they are known to be linked to neurons of the spinocervical
tract (SCT) (Brown 1981; Brown and Noble
1982
; Brown et al. 1986
). The SCT neurons ascend
ipsilaterally and synapse on neurons of the lateral cervical nucleus,
which then have a crossed projection, joining the medial lemniscal
outflow from the dorsal column nuclei (Brown 1981
). HFA
inputs are therefore known to project over at least two major parallel
ascending tactile pathways. In their linkage with the SCT neurons of
the dorsal horn, single HFA fibers can generate complex mono- and
polysynaptic EPSPs, which, in some cases, lead to spike output in the
target neuron (Brown et al. 1987a
-c
; Hongo and
Koike 1975
). Furthermore, the spike output may arise in burst
form, indicative of amplification at this dorsal horn synapse. Thus the
present results and the earlier observations at the dorsal horn
indicate that single HFA fibers have potent excitatory synaptic
linkages to at least two parallel ascending sensory systems. However,
whether the temporal precision of impulse patterning present in the HFA
responses to vibrotactile stimuli can be retained across the dorsal
horn synaptic junction with SCT neurons is uncertain. The evidence from
the study by Brown et al. (1987c)
suggests that this may
not be the case as the complex EPSPs evoked by single HFA input spikes
are protracted, leading to a temporal dispersion in the spike output over a period of 10-15 ms (see Figs. 9 and 10 in Brown et al. 1987c
). This temporal dispersion is far greater than the 2-3
ms observed for the spike bursts generated in cuneate neurons by single
HFA input spikes (see Figs. 3 and 4 in present paper) and would
presumably mean first, that phase locking of responses to vibrotactile
stimuli in SCT neurons would be considerably poorer than the levels
displayed by cuneate neurons, and second, that the capacity of the HFA
fiber-SCT neuron linkage to convey information about the frequency
parameter of vibrotactile disturbances in a temporal pattern code would
be poorer than that of the HFA fiber-cuneate neuron linkages.
![]() |
ACKNOWLEDGMENTS |
---|
We acknowledge the technical assistance of C. Riordan and D. Sarno.
This work was supported by the National Health and Medical Research Council of Australia and the Australian Research Council.
![]() |
FOOTNOTES |
---|
Address for reprint requests: M. J. Rowe (E-mail: M.Rowe{at}unsw.edu.au).
Received 23 February 2001; accepted in final form 17 April 2001.
![]() |
REFERENCES |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|