Department of Pharmacology and Toxicology, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-1031
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ABSTRACT |
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Yamada, Kei, Baojian Yu, and Joel P. Gallagher. Different subtypes of GABAB receptors are present at pre- and postsynaptic sites within the rat dorsolateral septal nucleus. GABAB receptor activation modulates neuronal activity mediated by multiple CNS transmitters and can occur at pre- and postsynaptic sites. In low concentrations, baclofen acts presynaptically to diminish transmitter release via both hetero- and autoreceptors, whereas at increasing concentrations, the same compound alters postsynaptic membrane excitability by inducing a membrane hyperpolarization.
We have utilized electrophysiological techniques in vitro
to focus on the possibility that pharmacologically different subtypes of GABAB receptors are present on presynaptic sites of
glutamatergic terminals when compared with GABAB receptors
on postsynaptic sites within the dorsolateral septal nucleus (DLSN).
The glutamatergic terminal within the DLSN originates from a pyramidal
cell body located within the hippocampus and most likely terminates on
a GABAergic neuron from which recordings were made. Whole cell patch voltage-clamp methods were employed to record pharmacologically isolated excitatory postsynaptic currents (EPSCs) from DLSN neurons as
an index of glutamatergic transmission. Using a modified internal pipette solution containing QX-314 and in which CsGluconate and GDPS
replaced Kgluconate and GTP, respectively, we recorded isolated monosynaptic EPSCs. The GABAA receptor antagonists
bicuculline and picrotoxin were included in the external standard
superfusion solution. Application of the GABAB receptor
agonists, (±)-baclofen, CGP44533, and CGP35024 (10 nM to 10 µM)
depressed glutamate-mediated EPSCs in a concentration-dependent manner.
With the use of this combination of solutions, CGP44533 did not produce
postsynaptic membrane property changes. Under these conditions, both
(±)-baclofen and CGP35024 still induced increases of postsynaptic
membrane conductance associated with an outward current. The
GABAB receptor antagonist CGP55845A (1 µM) blocked the
presynaptic CGP44533-mediated depressant effects of EPSCs, whereas
CGP35348 (100 µM) or barium (2 mM) was ineffective. Futhermore, both
CGP35348 (100 µM) and CGP55845A (1 µM) were effective in blocking
the postsynaptic conductance changes associated with baclofen and
CGP35024, whereas barium was ineffective.
Our results demonstrate a distinct pharmacology for GABAB agonists acting at putative subtypes of GABAB receptors located on presynaptic sites of a glutamatergic terminal versus GABAB receptors on postsynaptic sites of a DLSN neuron. Furthermore, our results also suggest a different pharmacology and/or coupling of a GABAB receptor to different effectors at postsynaptic sites within the DLSN. Thus there may be three or more pharmacologically distinct GABAB receptors or receptor complexes associated with DLSN neurons: at least one pre- and two postsynaptic. If this distinct pharmacology and GABAB receptor distribution also extends to other CNS structures, such differences could provide development of selective drugs to act at these multiple sites.
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INTRODUCTION |
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GABA receptors in the mammalian CNS are currently classified into
three pharmacological types: GABAA, GABAB, and
GABAC. Multiple neurochemical studies at a variety of CNS
preparations in which transmitter release has been monitored suggest
that subtypes of GABAB receptors are present on various
nerve terminals. Furthermore, molecular biological cloning of
GABAB receptors has provided additional support to the
concept of multiple GABAB receptors. The initial cloning of
a GABAB receptor that demonstrated the presence of two
splice variants (GABABR1a and GABABR1b)
(Kaupman et al. 1997), has recently been expanded by the
demonstration of a second and different GABAB clone:
GABABR2. This latter discovery was especially exciting in
that four different laboratories, almost simultaneously, reported their
similar results (Jones et al. 1998
; Kaupmann et al. 1998
; Kuner et al. 1999
; White et al.
1998
). Furthermore, the discovery of GABABR2
adds a new concept to the field of G protein-coupled receptors in that
the combination, as a heterodimer, of these two clones
(GABABR1 + GABABR2) is required when expressed in a cell line with an inward rectifier potassium channel
(GIRK) to observe full biological activity comparable with that
recorded from native tissue. It may be possible that the requirement
for this combination of two different clones may lead to the further discovery of additional GABAB receptors, which may involve
the formation of novel heterodimers.
A major function of GABAB receptors is to modulate
transmitter release (Alford and Grillner 1991;
Bowery et al. 1980
; Davies et al.
1991
; Kombian et al. 1996
;
Mouginot and Gähwiler 1996
; Thompson et al.
1993
). Activation of nerve terminal GABAB receptors inhibits the release of various transmitters including biogenic amines,
GABA, and glutamate. Reports have implicated CNS GABAB receptors with several CNS disorders, for example, epilepsy,
specifically absence seizures (Crunelli and Leresche
1991
; Malcangio and Bowery 1995
), drug abuse
(Roberts and Andrews 1997
; Roberts et al.
1996
; Shoji et al. 1997
), and other CNS
disorders (Bowery et al. 1997
). In animal models,
GABAB receptor antagonists block absence-epilepsy seizures,
whereas spike-and-wave discharges were suppressed by injection of
CGP35348 intraperitoneally (Smith and Fisher 1996
). Experiments by Roberts et al. (1996)
and Roberts
and Andrews (1997)
demonstrated that acute treatment with
baclofen suppressed cocaine self-administration in rats, whereas
Gudeman et al. (1996)
and Ling et al.
(1998)
reported that baclofen may act as a cocaine anti-craving
medication in cocaine-dependent individuals. These and other results
suggest that GABAB receptor agonists or antagonists could
prove to be potentially useful therapies for a variety of CNS
disorders. Because GABAB receptors exist in various parts of the nervous system, it is difficult to predict where
GABAB receptor agonists or antagonists act and whether they
do so selectively.
The possibility that multiple GABAB receptor subtypes are
found in various synapses in brain has been suggested (Beck et
al. 1995; Bonanno and Raiteri 1992
,
1993a
,b
; Calabresi et al. 1991
; Cunningham and Enna 1996
; Dutar and Nicoll
1988
; Gemignani et al. 1994
; Howe et al.
1987
; Pham and Lacaille 1996
; Pham et al. 1998
). In particular, using both baclofen and GABA, older
GABAB receptor antagonists (phaclofen, 5-OH-saclofen,
CGP35348) were shown to antagonize less effectively postsynaptic
responses to GABA versus similar responses due to exogenous application
of baclofen (Jarolimek et al. 1994
). More recently,
Pham et al. (1998)
suggested that there were two
distinct GABAB receptors located postsynaptically at
synaptic and extrasynaptic sites on hippocampal pyramidal cells, and
that these receptors were linked to two different K+ conductances.
In addition, application of expression cloning had demonstrated two
splice variants of the first GABAB receptor clone
(Bowery and Brown 1997; Kaupmann et al.
1997
). Data suggested that one splice variant
(GABAB1a) was located presynaptically, whereas the other
(GABAB1b) was located postsynaptically. Furthermore, Shoji et al. (1997)
suggested two different subtypes of
GABAB receptors within the same synapse at pre-and
postsynaptic sites. The results of Shoji's experiments are consistent
with several prior studies (Deisz et al. 1993
,
1997
; Dutar and Nicoll 1988
; Pitler and Alger 1994
; Thompson and
Gähwiler 1992
). These reports focused on the different
subtypes of GABAB receptor within GABAergic synapses. On
the other hand, there has been a limited number of reports
(Huston et al. 1995
; Yoon and Rothman
1991
; for review see Thompson et al. 1993
) that
investigated the pharmacology of GABAB heteroreceptors at
glutamatergic synapses. A major limitation has been the unavailability
of novel GABAB agonists other than baclofen, and potent antagonists.
In this study we demonstrate with electrophysiological techniques a
pharmacological distinction between subtypes of GABAB receptors at pre- versus postsynaptic sites in the dorsolateral septal
nucleus (DLSN). Our data also support the recent findings of
Pham et al. 1998, namely, that two postsynaptic
GABAB receptors are coupled differentially to potassium
channels. Thus this glutamatergic terminal onto presumptive GABAergic
neurons within the DLSN provides us with potentially three different
GABAB receptor subtypes. These are distinguished by their
pharmacological sensitivity to GABAB agonists and
antagonists; at least one presynaptic type with two others located postsynaptically.
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METHODS |
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Preparation of brain slices
Male Sprague-Dawley rats (100-250 g; Harlan) were decapitated,
and their brains were rapidly dissected out and placed in cold artificial cerebrospinal fluid (ACSF) of the following composition (in
mM): 117 NaCl, 4.7 KCl, 1.2 MgCl2, 2.5 CaCl2,
1.2 NaH2PO4, 11.5 glucose, and 25 NaHCO3, prebubbled with 95% O2-5%
CO2, pH 7.4. Transverse blocks of tissue containing the
septum were serially cut on a Vibroslice (model 752 M; Campden
Instruments, London), into 500-µm slices. A single slice was
submerged and held between two nylon nets in a recording chamber that
was superfused with ACSF bubbled with 95% O2-5%
CO2 at 30-32°C. Only neurons with stable membrane
potentials of 50 mV maintained over a minimum period of 10 min, and
that yielded postsynaptic potentials (or currents) evoked by electrical
focal fimbrial stimulation, were included in this study.
Recording from brain slices
DLSN neurons were recorded using the blind whole cell
patch-clamp recording technique (Blanton et al. 1989;
Zheng et al. 1995
). Whole cell patch recordings were
obtained with 5-7 M
electrodes filled with (in mM) 122 KGluconate,
5 NaCl, 0.3 CaCl2, 2 MgCl2, 1 ethylene
glycol-bis(
-aminoethylether)-N,N,N',N'-tetraacetic acid
(EGTA), 10 N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES), 5 Na2ATP, 2 guanosine
5'-O-(2-thiodiphosphate). Instead of using our standard pipette
solution, we used CsGluconate, GDP
S, and 3 mM QX-314 in place of
potassium gluconate and guanosine triphosphate (GTP). This combination
has been suggested to block postsynaptic potassium channel conductance
changes induced by GABAB receptor activation (Mclean
et al. 1996
; Nathan et al. 1990
; Whittington et al. 1992
). We assayed for the
effectiveness of GDP
S by testing for the loss of a serotonin
(5HT-1A) (Twery et al. 1991
) mediated hyperpolarization;
once lost other GTP-dependent responses (baclofen) would also be absent.
Current signals and applied voltages were generated and recorded with an Axoclamp 2A amplifier (Axon Instruments, Foster City, CA). The output amplifier was DC-coupled to a storage oscilloscope (model 220; Gould, Cleveland, OH). A model 4208 Panasonic videocassette recorder (A. R. Vetter, Redersburg, PA) was used to capture all tracings for off-line analysis and archiving. The stored signal could be played back and analyzed using pClamp (version 6.3.1) software, with a DigiData 1200 interface to a Gateway 2000 4DX2-66V computer. Orthodromic stimuli were delivered with square-wave pulses (4-40 V; 0.1 ms) via a concentric bipolar electrode placed focally within the DLSN.
Drug application
(±)-Baclofen, CGP44533, CGP35024, bicuculline methiodide,
picrotoxin, 6,7-dinitroquinoxaline-2,3-dione (DNQX),
D()-2-amino-5-phosphonopentanoic acid
(D-APV), CGP35348, and CGP55845A were all applied by bath superfusion to achieve steady-state concentrations within the recording
chamber. (±)-Baclofen, bicuculline methiodide, and picrotoxin were
obtained from Sigma. DNQX and D-APV were obtained from RBI. QX-314 bromide was supplied by ASTRA. Novartis (Basel) kindly supplied
CGP44533, CGP35024, CGP35348, and CGP55845A. (Note that CGP35024 is
identical to SKF97541. Figures, legends, and text will only refer to
CGP35024, although identical results have also been collected by us and
reported by others with SKF97541. All results reflect the effect of
drugs applied to only one neuron from each brain slice.)
Data analysis
A concentric electrode positioned focally at the dorsolateral
horn of the septum (where hippocampal input entered via the fimbria)
evoked synaptic currents. The intensity of a test stimulus was chosen
to yield a half-maximal amplitude excitatory postsynaptic current
(EPSC). Changes in postsynaptic conductance resulting from drug
application were monitored by application of a hyperpolarizing voltage
step (5 mV). EPSCs were isolated by using the modified internal
solution with addition of bicuculline (10 µM) and picrotoxin (10 µM) to the standard external solution. Individual EPSCs were recorded
at 0.3 Hz, with 10 EPSCs collected and averaged. Data from different
slices were pooled and presented as means ± SE.
GABAB agonists were added in the superfusion solution and applied for 5-10 min. GABAB receptor antagonists were applied in advance of GABAB receptor agonists for 10 min and then co-applied with agonists.
Statistical analyses employed a one-way repeated measures ANOVA using
SigmaStat for Windows, V. 1.0. Multiple comparisons were made to
control values using Dunnett's test for statistical significance
determined at the level of P < 0.05, when
T = 0.05. Graphs were generated using SigmaPlot (Windows
version 4.0) software (SPC Software, Santa Clara, CA), with a Hewlett
Packard LaserJet 4 printer.
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RESULTS |
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Isolation of EPSCs using a modified pipette solution and GABAA receptor antagonists in the external solution
Results were obtained from 52 DLSN neurons using blind whole cell
patch-clamp recording. These cells had resting potentials ranging from
58 to
76 mV (
66.0 ± 0.6 mV, mean ± SE). Input resistance of the cells ranged between 75.0 and 625 M
(233.4 ± 24.9 M
, n = 44). Fimbrial stimulation produced
triphasic postsynaptic currents, consisting of a fast-EPSC,
fast-inhibitory postsynaptic current (f-IPSC), and slow-inhibitory
postsynaptic current (s-IPSC) (Fig.
1A). The f-IPSC was completely
blocked by the combination of bicuculline (10 µM) and picrotoxin (10 µM). The s-IPSC was blocked by CGP35348 (100 µM) or CGP55845A (1 µM) (Bon and Galvan 1996
; Pham et al.
1998
) or by barium (1 mM; Fig. 1B) (Pham et al. 1998
). Using sharp recording electrodes, we previously
reported that the s-IPSC in the DLSN was also blocked by barium (2 mM) (Yamada and Gallagher 1997
).
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f-IPSCs and s-IPSCs need to be blocked to investigate monosynaptic
glutamatergic transmission (Fig. 1D). Elimination of these two IPSCs was achieved by modification of the standard pipette solution
and external ACSF, while not including a GABAB antagonist in the superfusion solution. Addition of GABAB antagonists
would mask a potential, selective presynaptic action of
GABAB agonists. K-gluconate was replaced with CsGluconate
to block potassium-sensitive channels coupled to the GABAB
postsynaptic receptor, and GTP was replaced with GDPS to uncouple
the postsynaptic G protein coupled to its effector, a potassium
channel. QX-314 was added to block action potentials evoked by
supramaximal EPSCs and to block potassium channels coupled to the
postsynaptic GABAB receptor (Nathan et al.
1990
). In our hands, the use of either QX-314 or GDP
S alone was inadequate to block the s-IPSC. Furthermore, this combination was
unable to block a baclofen (or CGP35024)-induced postsynaptic membrane
hyperpolarization (see Effects of GABAB agonists on
postsynaptic membrane conductance and Fig. 4). Bicuculline
(10 µM) and picrotoxin (10 µM) were applied in the external
solution to eliminate f-IPSCs. This combination of antagonists
exhibited no change in holding current or conductance. Immediately
after impalement with the electrode containing the modified pipette
solution, the postsynaptic response consisted of a series of triphasic
postsynaptic currents (Fig. 1A). After 30 min of impalement
with the modified internal solution in the recording electrode and
application of bicuculline and picrotoxin, an EPSC was isolated (Fig.
1D). The amplitude of isolated EPSCs ranged between 25.8 and
173.8 pA (92.4 ± 5.3 pA; n = 68) and were blocked
completely by DNQX (50 µM) and D-APV (25 µM).
Activation of presynaptic GABAB receptors depressed EPSCs
To study the function of presynaptic GABAB receptors
in glutamatergic synapses, we used the modified internal pipette
solution (see above and METHODS), while bicuculline and
picrotoxin were applied in the external solution. Upon elimination of
all IPSCs evoked by fimbrial stimulation, a series of three consecutive monosynaptic EPSCs were recorded and served as controls. After these
control EPSCs were recorded, (±)-baclofen (10 nM to 10 µM) was
applied to the external ACSF solution for 6-10 min. During this
period, postsynaptic membrane properties (membrane conductance and
holding current) were monitored continuously while applying a negative
cathodal voltage step command (5 mV).
Bath application of (±)-baclofen (10 nM to 10 µM) depressed the
amplitude of EPSCs in a concentration-dependent manner (Fig. 2, top, n = 10).
(±)-Baclofen at 10 nM depressed EPSCs by 11.4 ± 7.2%
(q' = 1.27; P = 3; N.S.) whereas at 10 µM,
EPSCs were depressed by 81.4 ± 5.3% (q' = 8.02;
P = 5; P 0.05). The onset of the depressant effect was observed after 3-5 min, whereas recovery occurred only after 10-30 min of wash out. Another GABAB
receptor agonist, CGP44533 (Froestl et al. 1995a
), which
is less potent when compared with (
)-baclofen in binding studies
(IC50s = 152 and 32 nM, respectively), was applied in
the same manner. At 10 nM, CGP44533 depressed EPSCs by 0.4 ± 3.7% (q' = 0.03; P = 3; N.S.), whereas at
10 µM, EPSCs were depressed by 78.2 ± 5.5% (q' = 6.64; P = 5; P
0.05; Fig. 2,
middle). The presynaptic inhibitory potency of (±)-baclofen
and that of CGP44533 were very similar (Fig.
3).
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CGP35024 is reported to be one of the most potent GABAB
receptor agonists, being 10 times more potent than (±)-baclofen
(Froestl et al. 1995a). CGP35024 (10 nM) depressed EPSCs
by 28.2 ± 11.4% (q' = 2.03; P = 3;
N.S.), and at 10 µM depressed EPSCs by 86.2 ± 4.5%
(q' = 7.32; P = 5; P
0.05; Fig. 2, bottom). The depressant effect induced by
CGP35024 was observed after 3 min exposure, and recovery occurred only
after >30-60 min of wash out. The IC50 of CGP35024 was 10 times less than that of (±)-baclofen or CGP44533 (Fig. 3).
Presynaptic depressant effect on EPSCs induced by CGP44533 persists in the presence of different GABAB receptor or channel antagonists, but not in CGP55845A
There are three reports describing the sensitivity of postsynaptic
GABAB receptors to GABAB receptor agonists or
antagonists within the DLSN (Bon and Galvan 1996;
Hasuo and Gallagher 1988
; Stevens et al.
1985
). But the sensitivity of presynaptic GABAB receptors on glutamatergic neurons (heteroreceptors) to
GABAB receptor agonists or antagonists has not been
reported. On the basis of our results showing a lack of postsynaptic
conductance change by CGP44533 (Fig. 4),
we utilized the CGP44533-induced presynaptic depression of
orthodromically induced EPSCs as an index of GABAB
antagonist efficacy at presynaptic GABAB receptors. After
10 min pretreatment with GABAB receptor or channel
antagonists, CGP44533 was co-applied with three different antagonists
for 6-10 min. During application of barium (2 mM), at a concentration
sufficient to eliminate the s-IPSCs in the DLSN, CGP44533 (10 nM to 10 µM) still depressed EPSCs in a concentration-dependent manner (Figs. 5 and
6, n = 6). We assayed
for the effectiveness of barium by monitoring the prolongation of a
direct cathodally induced action potential. In the absence of barium,
CGP44533 (10 nM) depressed EPSCs by 0.43 ± 3.7%, whereas at 10 µM it depressed EPSCs by 78.2 ± 5.5%. Similarly, but in the
presence of barium, CGP44533 (10 nM) continued to depress EPSCs by
0.9 ± 5.5%, whereas at 10 µM it also depressed EPSCs by
75.3 ± 7.4%. CGP35348 (100 µM), at a concentration sufficient
to block baclofen-induced hyperpolarizations and s-IPSCs (Bon
and Galvan 1996
; Yamada and Gallagher 1997
), did
not suppress the ability of CGP44533 to induce its presynaptic depressant effect (Figs. 5 and 6, n = 8). There was no
statistical difference between results obtained in the presence or
absence of CGP35348 (P > 0.05). Furthermore, all cells
showed slightly potentiated EPSCs, +5.5 ± 2.2% at 1 µM
(q' = 3.10, P = 4, P
0.05); and +4.9 ± 1.7%, at 10 µM (q' = 10.69, P = 4, P
0.05) in the presence of
CGP55845A after 6-10 min co-application of CGP44533 and CGP55845A
(Fig. 6, bottom). This potentiation of the EPSC by CGP55845A
may have been due to its disinhibition of presynaptic GABAB
receptor function by CGP55845A. CGP44533 by itself or co-application of
CGP44533 with CGP35348, CGP55845A, or barium did not affect
postsynaptic membrane properties under these recording conditions (data
not shown). However, using sharp electrodes filled with KAc, we have
recorded a direct postsynaptic membrane hyperpolarization associated
with a conductance increase after superfusion with CGP44533 (10 µM)
(Yamada and Gallagher 1997
); this postsynaptic action of
CGP44533 was blocked by CGP33548 (100 µM). We have repeated these
experiments using the whole cell recording technique and demonstrated
(Fig. 1C) the ability of CGP44533 to induce an outward
current using a control pipette recording solution; this response and
its associated increase in conductance is lost with the modified
pipette recording solution (Fig. 4).
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Effects of GABAB agonists on postsynaptic membrane conductance
When focusing on the postsynaptic membrane conductance changes induced by GABAB receptor agonists, we observed that (±)-baclofen and CGP35024 continued to produce a concentration-dependent increase of postsynaptic membrane conductance, even under conditions when using the modified internal pipette solution with externally applied bicuculline and picrotoxin.
(±)-Baclofen (10 nM) produced a 11.8 ± 4.5% increase, whereas
at 10 µM it produced a 35.8 ± 14.5% increase of postsynaptic membrane conductance (Fig. 4). Similarly, CGP35024 (10 nM) produced a
1.6 ± 1.5% change, and at 10 µM it produced a 20.4 ± 6.0% increase of postsynaptic membrane conductance (Fig. 4). Under
these recording conditions, both baclofen and CGP35024 still induce a
concentration-dependent and persistent outward current (Fig. 4,
A-C) associated with an increased postsynaptic conductance.
CGP35348 of CGP55845A blocked this conductance change induced by each
of these GABAB agonists, whereas barium was ineffective
(Yamada and Gallagher, unpublished data). These results suggest that
(±)-baclofen and CGP35024 activate GABAB receptors on the
postsynaptic membrane that may be coupled to potassium channels that
are Cs and Ba insensitive, while also QX-314 resistant. Results with
these GABAB agonists applied exogenously differ from data
collected with blockade of the s-IPSC. A distinction between blockade
of synaptic responses versus inability to block exogenous application
of GABA responses has been reported in CA1 of the hippocampus
(Pham and Lacaille 1996). Subsequently (Pham et
al. 1998
), these authors have suggested a differential
distribution of GABAB receptors coupled to different
K+ channels that are present at subsynaptic versus
extrasynaptic sites. Synaptically released GABA could activate only the
subsynaptic sites, whereas exogenous superfusion of GABA and/or GABA
released by high-intensity or high-frequency stimuli could activate
both receptors (however, see Pham et al. 1998
). Another
possibility is that the receptors are similar but exhibit a different
pharmacology due to a difference in their effectors or coupling machinery.
In terms of postsynaptic conductance changes, only CGP35024 and baclofen, not CGP44533, continued to produce significant conductance changes even under the conditions of using the modified internal pipette solution with externally applied bicuculline and picrotoxin (Fig. 4). CGP44533 at 10 nM produced a 7.6 ± 3.6% increase in conductance, whereas at 10 µM only a 7.2 ± 12.6% change was recorded. These data suggest that CGP44533, under our recording conditions, is a selective presynaptic GABAB heteroreceptor agonist at glutamatergic nerve terminals.
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DISCUSSION |
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We investigated the effects of GABAB receptor agonists
and antagonists on monosynaptic glutamatergic transmission in the DLSN. Focal fimbrial stimulation of the DLSN slice preparation produced a
composite of triphasic synaptic currents, namely, an EPSC, f-IPSC, and
s-IPSC. To isolate monosynaptic glutamatergic transmission, inhibitory
postsynaptic responses were eliminated by the use of a modified pipette
solution including cesium, GDPS and QX-314, with external
application of bicuculline and picrotoxin. After 30 min of impalement,
the f-IPSC and s-IPSC were absent, and an isolated EPSC became
apparent. The EPSC was blocked completely by DNQX and
D-APV. These results suggested that this isolated EPSC was
mediated by non-NMDA and NMDA receptors, which is consistent with our
previous report (Gallagher and Hasuo 1989
.)
Under these experimental conditions, bath application of three GABAB receptor agonists (±)-baclofen, CGP44533, and CGP35024 acted in a concentration-dependent manner to depress the amplitude of the EPSC. CGP35024 was 10-fold more potent than the 2 other GABAB receptor agonists in this EPSC depressant action. However, only CGP44533 produced this presynaptic action in the absence of any significant postsynaptic membrane changes.
GABAB antagonists differentiate between presynaptic GABAB receptors and postsynaptic GABAB receptors
Two GABAB receptor antagonists, CGP35348 (100 µM),
CGP55845A (1 µM) (Bon and Galvan 1996), or barium (2 mM) (Yamada and Gallagher 1997
) depressed
baclofen-induced membrane hyperpolarizations and blocked the s-IPSP in
the DLSN. The most potent GABAB receptor antagonist tested,
CGP55845A, not only blocked the CGP44533 depressant action on EPSCs,
but it also resulted in a potentiation in the amplitude of EPSCs. These
results suggest that CGP55845A blocked the CGP44533-induced depression
of EPSCs by blocking the presynaptic GABAB receptor and
increasing glutamate release. A similar result has been reported in the
supraoptic nucleus (Kombian et al. 1996
). There, another
GABAB receptor antagonist, CGP36742, also enhanced EPSCs
significantly without exhibiting any postsynaptic membrane changes or
altering postsynaptic responses induced by exogenous application of
glutamate agonists (Froestl et al. 1995b
; Kombian et al. 1996
). This result adds further support for a
differential GABAB pharmacology at glutamatergic terminals.
Neither CGP35348 nor barium altered the ability of CGP44533 to depress
EPSCs in the DLSN. Barium has been shown to be ineffective in blocking
the presynaptic actions of baclofen at other sites (Thompson et
al. 1993). On the other hand, CGP35348 has been reported to
block the release of glutamate and somatostatin in cerebral cortex
synaptosomes (Bonanno and Raiteri 1993b
).
Together these data indicate that, within glutamatergic synapses, the
sensitivity of CGP35348 varies, depending on the respective sites
examined. Our data support the earlier suggestions (Bonanno and
Raiteri 1993a
) that there are at least two kinds of
GABAB heteroreceptor subtypes on glutamate nerve terminals
in the CNS, each differing in their sensitivity to CGP35348. Bonanno
and Raiteri went on to propose a classification to differentiate
GABAB receptors. GABAB1 are baclofen sensitive,
whereas GABAB2 are baclofen insensitive, but
GABA-sensitive. Pham et al. 1998
have extended this
differentiation to suggest that GABAB1 are coupled to
barium-sensitive K+ conductances and are found at
subsynaptic sites, whereas GABAB2 are coupled to
barium-insensitive K+ conductances found at extrasynaptic sites.
The fact that inhibition of glutamate release induced by all three
GABAB agonists tested in the DLSN also persists in the presence of extracellular barium supports the concept that calcium, rather than potassium channels (Takahashi et al. 1998),
mediate this inhibitory action in the DLSN. Baclofen has been shown to depress specific types of calcium currents differentially in
thalamocortical neurons. N-, L-, and R-type calcium currents were
recorded, with the following depression of each of these currents
observed: 0, 30, and 100% depression of L, N, and R, respectively
(Guyon and Leresche 1995
). CGP35348 (100 µM) or
CGP55845A (100 nM) reversed depression by baclofen of the N-type
current. On the other hand, only CGP55845A (100 nM) blocked the
baclofen effect on the R-type current; CGP35348, up to 100 µM, was
ineffective (Guyon and Leresche 1995
). It is possible
that presynaptic GABAB receptors within the DLSN may also
be coupled with R-type calcium channels because the presynaptic
depression by baclofen was not blocked by CGP35348, but was sensitive
to CGP55845A. Another possibility is that a nerve terminal
GABAB receptor may be coupled to a barium-resistant K+-channel, and thus is similar to a barium-resistant
GABAB receptor complex postulated for the postsynaptic membrane.
The existence of multiple subtypes of GABAB receptors has
been suggested in the CNS (Bonanno and Raiteri 1993a;
Dutar and Nicoll 1988
; Pham and Lacaille
1996
). Many reports have focused on differences between pre-
and postsynaptic GABAB receptors (Deisz et al.
1993
, 1997
; Dutar and Nicoll
1988
; Pitler and Alger 1994
; Thompson and
Gáhwiler 1992
). We report different subtypes of GABAB receptor at an excitatory glutamatergic terminal
within the DLSN. In the DLSN, although the postsynaptic
GABAB receptor responsible for the synaptically mediated
s-IPSC is sensitive to barium, CGP35348, and CGP55845A, the presynaptic
GABAB receptor is only sensitive to CGP55845A and not to
barium or CGP35348.
However, over the same concentration range, both CGP35024 and
(±)-baclofen also produced postsynaptic membrane conductance increases. These latter results are consistent with a previous report
( Bon and Galvan 1996) that demonstrated the following IC50s for these agonists to induce a membrane
hyperpolarization in the DLSN: CGP35024 (0.05 µM) and (±)-baclofen
(0.55 µM). The reversal potential of the membrane hyperpolarization
induced by (±)-baclofen or CGP35024 in DLSN neurons was the same as
the equilibrium potential of potassium (Bon and Galvan
1996
; Stevens et al. 1985
). This result
indicates that postsynaptic GABAB receptors in the DLSN are
coupled solely with potassium channels. However, even though
CsGluconate, QX-314, and GDP
S were employed to eliminate postsynaptic GABAB responses, both baclofen and CGP35024
still caused an increase in membrane conductance and an associated
outward current at a time when the synaptic s-IPSC was blocked. We have conducted extrapolated reversal potentials of these outward currents, which yield values between
90 to
100 mV; these data suggested a
K+ reversal. Experiments with altered extracellular
[K+] concentrations will be conducted to verify this.
These latter, planned experiments are especially critical due to the
high cesium and gluconate in our recording electrode; the high cesium
may pass through K+ channels and affect our results,
whereas the high gluconate may produce a large D.C. offset that is
difficult to compensate. We will conduct a thorough analysis of the
reversal potential of these responses as part of another study.
These data suggest that there are two types of potassium channels
coupled to a postsynaptic GABAB receptor in the rat DLSN. One is sensitive to intracellular cesium, QX-314, and/or GTPS. This
GABAB receptor is intrasynaptic and responsible for
mediating the synaptic response. Another is insensitive to
intracellular cesium and QX-314, extracellular barium, and possibly
coupled to its receptor via a membrane delimited G protein; this latter receptor is situated extrasynaptic. Baclofen-activated
cesium-insensitive potassium channels have been reported (Wagner
and Dekin 1993
, 1997
). These cesium-insensitive
potassium channels are classified as outward rectifying or "KORs".
We propose that the coupling mechanism for KORs may be membrane
delimited (Hille 1992
; Isaacson and Hille
1997
), a property that could explain why the response we
recorded persisted even when the cell is filled with GDP
S. KORs are
distinguished from the more common inwardly rectifying potassium
channels or "GIRKs," which are cesium sensitive. Taken together,
these data could also explain the results of Pham and Lacaille
(1996)
, who reported a barium-resistant postsynaptic response
at a time when the synaptic s-IPSC was blocked; they (Pham et
al. 1998
) suggested that this barium-resistant response is
situated extrasynaptically.
A teleological question may be asked of why have two postsynaptic GABAB receptors coupled to different potassium channels. One suggestion could be that they are situated separately to function under different circumstances; one, when little GABA is released within the synapse, and the other, when excessive GABA is released. Under this latter situation when the synaptic receptors are saturated, and if additional inhibition is required, the extrasynaptic receptors are activated to enhance/prolong inhibition.
These results support the concept that there are at least three pharmacologically different subtypes of GABAB receptors present within a glutamatergic DLSN synapse; two are located at postsynaptic sites, whereas a third is found on the nerve terminal. It may be possible that a similar distribution of GABAB receptors could be found at other CNS synapses.
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ACKNOWLEDGMENTS |
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We are grateful to Dr. W. Froestl for reviewing this manuscript and supplying critical Novartis compounds.
The National Institutes of Health and the John Sealy Foundation supported this work.
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FOOTNOTES |
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Address reprint requests to J. P. Gallagher.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Received 15 May 1998; accepted in final form 22 February 1999.
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REFERENCES |
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