Neuroscience Research Group, Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta T2N 4N1, Canada
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ABSTRACT |
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Price, Christopher J. and Quentin J. Pittman. Dopamine D4 Receptor Activation Inhibits Presynaptically Glutamatergic Neurotransmission in the Rat Supraoptic Nucleus. J. Neurophysiol. 86: 1149-1155, 2001. Oxytocin and vasopressin release from magnocellular neurons of the supraoptic nucleus is under the control of glutamate-dependent excitation. The supraoptic nucleus also receives a generalized dopaminergic input from hypothalamic sources. To determine if dopamine can influence this excitatory drive onto the magnocellular neurons, we used whole-cell patch clamp to record the effect of dopamine on evoked and miniature excitatory postsynaptic currents in rat hypothalamic slices. Dopamine exposure (30 µM to 1 mM) induced a large and reversible reduction in the amplitude of evoked excitatory postsynaptic current in nearly all magnocellular cells tested. D4 receptors appeared to mediate dopamine's activity, based on inhibition of the response with 50 µM clozapine, but not by SCH 23390 or sulpiride, and mimicry of dopamine's action with the D4 specific agonist, PD 168077. Analysis of paired-pulse experiments and miniature postsynaptic currents indicated that dopamine's action involved a presynaptic mechanism, since the frequency of miniature postsynaptic currents was reduced with dopamine exposure without any change in current kinetics or amplitude, while the paired-pulse ratio increased. We therefore have demonstrated for the first time a role for dopamine D4 receptors in the supraoptic nucleus in the presynaptic inhibition of glutamatergic neurotransmission onto magnocellular neurons.
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INTRODUCTION |
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Dopamine is one of
the major modulatory neurotransmitters in the CNS (Jaber et al.
1996). To achieve this function, dopamine binds to one of two
families of G-protein-coupled receptors (Missale et al.
1998
; Seeman and Van Tol 1994
). The D1 family
consists of D1 and D5 receptors and, in general, is positively coupled to adenylate cyclase activity. The D2 family consists of D2, D3, and D4
receptors and is negatively coupled to adenylate cyclase activity. D1
and D2 receptors have a widespread distribution throughout the nervous
system, while D3, D4, and D5 receptors tend to have a more restricted
distribution (Defagot et al. 1997
, 2000
; Jaber et
al. 1996
; Levant 1997
). In regards to cellular
localization of the receptor, all subtypes can be found either
presynaptically or postsynaptically, allowing dopamine to exert its
neuromodulatory effects on both sides of the synapse
(Behr et al. 2000
; Jaber et al. 1996
;
Koga and Momiyama 2000
; Levant 1997
;
Nicola et al. 2000
; Svingos et al. 2000
).
The supraoptic nucleus (SON) of the hypothalamus contains oxytocinergic
and vasopressinergic magnocellular neurons. The magnocellular neurons
receive glutamatergic inputs resulting in AMPA and NMDA receptor-dependent excitation of the cells and subsequent oxytocin or
vasopressin release from the posterior lobe of the pituitary (Armstrong 1995; Stern et al. 1999
).
Among a variety of other inputs, the SON receives a diffuse and
generalized dopaminergic input from neurons from the A14 and A15 cell
groups (van Vulpen et al. 1999
). In the SON,
dopaminergic fibers form synaptic terminals near both the dendrites and
the cell bodies of magnocellular neurons (Buijs et al.
1984
; Decavel et al. 1987
). While the presence
of dopamine in the SON is clear, less straightforward is the role that
dopamine plays in the modulation of magnocellular neuron function.
Previous reports have described the effect of dopamine on the release
of oxytocin in lactating rats to be either excitatory, via a D1-like
receptor-dependent mechanism, or inhibitory, likely via a D2-like
receptor-dependent mechanism (Crowley et al. 1987
; Parker and Crowley 1992
). Furthermore, dopamine has also
been shown to depolarize magnocellular neurons in SON explants from male rats in a D2-like receptor-dependent manner (Yang et al. 1991
).
Most of the studies examining dopamine actions on SON magnocellular neurons describe general effects on SON output, while little is known about the effects of dopamine on specific ionic conductances. To look for a specific action of dopamine on the modulation of magnocellular neuron excitation, we directed our attention to examining for a specific effect of dopamine on excitatory synaptic responses. Using hypothalamic slices from male rats, whole-cell patch-clamp recordings demonstrated that dopamine exposure resulted in a reduction in the amplitude of evoked excitatory postsynaptic currents (EPSCs) in virtually all neurons tested. Furthermore, this action was pharmacologically attributed to the activation of dopamine D4 receptors, which is consistent with immunocytochemical studies showing D4 receptors to be highly expressed in the SON.
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METHODS |
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Preparation of hypothalamic slices
Coronal hypothalamic slices (400 µm) containing the SON were obtained from male Sprague-Dawley rats (130-230 g, U. Calgary Biosciences and Charles River). Briefly, rats were anesthetized with halothane and decapitated, and the brain quickly removed, trimmed, and mounted on a vibratome in ice-cold dissecting solution (in mM: 25 NaCl, 2.5 KCl, 1 CaCl2, 4 MgCl2, 1.2 NaH2PO4, 18 NaHCO3, and 200 sucrose), which was constantly bubbled with 95% O2-5% CO2. Slices were then incubated for at least 2 h at room temperature before being hemisected and transferred into a recording chamber where the slice was constantly perfused with artificial cerebrospinal fluid (ACSF) (in mM: 126 NaCl, 2.5 KCl, 1.2 NaH2PO4, 1.2 MgCl2, 2.4 CaCl2, 18 NaHCO3, and 17 glucose). ACSF was heated to 28-32°C and constantly bubbled with 95% O2-5% CO2. To prevent contamination of EPSCs with GABA-mediated currents, 50 µM picrotoxin was added to the perfusing ASCF.
Electrophysiology and analysis
Blind whole-cell patch recordings were then made from
electrophysiologically identified SON magnocellular neurons. Neurons were identified as being magnocellular neurons by the presence of a
delay in the onset of action potential initiation with depolarizing current pulses during current clamp recordings (Hoffman et al. 1991; Kombian et al. 2000
). Further, in several
of the recordings, Lucifer yellow (Polysciences) was included in the
pipette solution. Subsequent viewing of the slices revealed that all of
the filled cells resided within the supraoptic nucleus (data not
shown). Electrodes used for whole-cell recordings were pulled from
thin-walled borosilicate capillary tubes and had resistances between 2 and 7 M
when filled with recording solution (130 mM K-gluconate, 10 mM KCl, 10 mM NaCl, 1 mM MgCl2, 10 mM EGTA, 2 mM
Mg-ATP, 0.3 mM Li-GTP, 1 µM Leupeptin, and 10 mM HEPES; pH 7.3 with
KOH). Leupeptin was included in this solution to improve recording
stability (Kay 1992
). EPSCs were evoked using a tungsten
bipolar electrode placed dorsomedial to the SON, using voltage pulses
that typically were between 3 and 30 V and 600 µs in duration.
Following each evoked event, a hyperpolarizing voltage pulse was
applied to the cell to assess access resistance. EPSCs were recorded at
a holding potential of
70 mV, using an Axon 200A amplifier and
Clampex 6.0 software (Axon Instruments). For evoked EPSCs,
stimuli-eliciting responses of 50-60% maximum were delivered once
every 15 s and four consecutive responses were averaged. Data were
acquired at a sampling rate of 10 kHz and filtered at 5 kHz. Data from
evoked EPSC experiments were measured using Clampfit 6.0 (Axon
Instruments) and exported to Prism 3.0 (GraphPad) where plots were made
and statistical analysis performed. Experiments examining miniature excitatory postsynaptic currents (mEPSC) were performed in standard ACSF. As several laboratories (Kabashima et al. 1997
;
Kombian et al. 2000
) have found no difference in the
frequency or amplitude of spontaneous EPSCs recorded in the presence or
absence of tetrodotoxin, these can be validly designated as mEPSCs.
mEPSCs were sampled at 5 kHz and filtered at 1 kHz. For analysis of
mEPSCs, data were exported to Mini Analysis Program (Synaptosoft,
v.4.3.3) for analysis and production of figures. Only events with
amplitudes >5 pA and rise times <5 ms were counted. The statistical
test used, except where indicated, was one-way analysis of variance
(ANOVA) with Bonferroni multiple comparison post hoc test. In the text,
values are presented as mean ± SE, and statistical significance
was determined when P < 0.05.
Immunohistochemistry
Slices containing Lucifer yellow-filled cells were fixed in 20% formalin (BDH) for at least 24 h at 4°C and then rinsed with Tris-buffered saline. Slices were permeabilized with 0.1% Triton X-100 and nonspecific staining blocked with 0.005% bovine serum albumen and 10% normal goat serum (Vector). Anti-oxytocin/neurophysin (1:1000, ATTC) was applied for 72 h at 4°C, after which a Cy3-labeled goat anti-mouse secondary antibody (Biological Detection Systems) was used (1:1000).
Drugs
During experiments, drugs were bath applied at the concentrations indicated. Stock solutions for all drugs were prepared fresh daily and diluted just prior to bath application, with the exception of CNQX, which was stored as a frozen 1000-fold stock solution in DMSO. Dopamine and SKF 81297 stock solutions both included 1 mg/ml sodium metabisulfite to prevent oxidation. In all instances where ethanol or DMSO was used to dissolve drugs, the final stock solution concentration was always 1000-fold the working concentration. All chemicals were obtained from Sigma/RBI, with the exception of PD 168077 (Tocris Cookson and Sigma).
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RESULTS |
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Dopamine inhibits evoked glutamatergic EPSCs
During whole-cell recordings from SON magnocellular neurons, EPSCs could reliably be evoked by stimulation of glutamatergic afferents. Exposure of slices to 100 µM dopamine for 5 min resulted in a marked reduction in the amplitude of evoked CNQX-sensitive EPSCs to 45 ± 5% of control values (n = 17). The response could often be seen to start within approximately 2 min of starting the flow of dopamine-containing ACSF and peaked at between 4 and 5 min. This reduction in EPSC amplitude was observed in all but two neurons tested, was completely reversible on washing, and was repeatable with reapplication of dopamine (Fig. 1). Furthermore, immunostaining with antibodies against oxytocin-neurophysin of neurons that had been labeled during recording with Lucifer yellow indicated that both stained and unstained neurons, presumably vasopressinergic neurons, responded to dopamine (data not shown). On scaling the EPSC recorded in dopamine back to its original amplitude, it was apparent that the kinetic properties of the EPSC were not altered (Fig. 1A). Measurement of the decay time constant before and in the presence of dopamine revealed no significant difference (paired t-test, P = 0.8795).
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Dose response experiments revealed that the receptor responsible for
dopamine-dependent reduction in the evoked EPSC had an EC50 of approximately 29 µM, a threshold
concentration above 10 µM, and maximal responses at concentrations
above 100 µM (Fig. 1B). In addition to the changes in EPSC
amplitude brought on by dopamine, in most cells dopamine application
resulted in a small inward shift in holding current (13.7 ± 2.5 pA; n = 24). However, the action of dopamine on the
EPSC was not correlated to the magnitude of the dopamine-induced inward
current (R2 = 0.1933).
D4 receptors mediate dopamine-dependent inhibition of evoked EPSCs
To determine the pharmacological identity of the receptor
responsible for dopamine-dependent reduction in EPSC amplitude, the
activities of various dopamine receptor agonists and antagonists were
tested. In the presence of the D1/D5 receptor antagonist SCH 23390 (50-100 µM), at concentrations over those needed to inhibit
D1/D5-mediated actions of dopamine in striatal slices (Umemiya
and Raymond 1997), dopamine was still fully capable of reducing
the amplitude of evoked EPSCs (Fig.
2A). Similarly, the D2/D3
receptor antagonist (
)sulpiride (10 µM), at a concentration where D2 receptor-mediated actions were blocked in slice experiments on
neurons from other areas (Chen et al. 1999
; Koga
and Momiyama 2000
; Shen and Johnson 2000
), was
ineffective in inhibiting dopamine-dependent reduction in the
evoked EPSC (Fig. 2B). Nonetheless, sulpiride did block the
dopamine-induced inward shift in holding current (
1.9 + 3.9 pA;
n = 7). From these results, it appeared that the receptor involved was neither D1-like nor D2-like. However, since both
of these antagonists are only weakly active at D4 receptors, the
atypical antipsychotic clozapine was also tested, as this compound has
10-fold greater activity at D4 receptors than at either D2 or D3
receptors (Seeman and Van Tol 1994
). When 100 µM
dopamine was applied in the presence of 50 µM clozapine,
dopamine-dependent reduction in EPSC amplitude was inhibited (Fig.
2C). This was a specific activity of clozapine at dopamine
receptors, since a serotonin-dependent reduction of EPSC amplitude
still progressed as normal in the presence of clozapine (data not
shown). To confirm the D4 identity of the receptor involved,
experiments were also performed using the specific D4 antagonist
L750,667 (50 µM) (Patel et al. 1996
). One-way ANOVA
demonstrated that only clozapine and L750,667 significantly prevented
dopamine-dependent inhibition of evoked EPSC amplitude (Fig.
2D). Since dopamine can also interact with adrenergic
receptors, we performed experiments where 100 µM dopamine was exposed
to slices preincubated with the general
-adrenergic antagonist
phentolamine (1 µM). Under these conditions, dopamine still caused a
mean reduction to 73 ± 9% of the original evoked EPSC amplitude,
which was not significantly different from the activity of dopamine in
the absence of phentolamine (P > 0.05, n = 4).
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The experiments using dopamine-receptor antagonists suggested that the
pharmacological identity of the dopamine receptors involved in
dopamine-dependent reduction in EPSC amplitude were D4 receptors. To
address this possibility further, dopamine-receptor agonists with
specificity for the different receptor subtypes were tested for their
ability to mimic dopamine's actions. The D1 receptor full-agonist SKF
81297 (10-30 µM) failed to mimic dopamine at reducing EPSC
amplitude, consistent with the inactivity of the D1 antagonist, SCH
23390 (Fig. 3A). The general
D2 receptor agonist quinpirole (50-200 µM), while mimicking
dopamine's action on the inward current (6.5 ± 3.423 pA;
n = 7), was ineffective at mimicking the action of
dopamine on the evoked EPSC (Fig. 3B). However, exposure of
magnocellular neurons to the recently developed specific D4 agonist PD
168077 (Glase et al. 1997; 30 µM) resulted in the
reduction of EPSC amplitude to a similar extent to that seen with 100 µM dopamine (Fig. 3C). ANOVA confirmed that the only
agonist whose performance was not significantly different from that of
dopamine's was PD 168077 (Fig. 3D). Therefore, from the
results of experiments with both dopamine receptor antagonists and
agonists, the identity of the dopamine receptor responsible for
reduction in EPSC amplitude is consistent with it being a D4 receptor.
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Dopamine presynaptically inhibits glutamate release
Dopamine receptors have been demonstrated to occur on both presynaptic and postsynaptic membranes. To determine if the locus of dopamine-dependent inhibition of EPSC amplitude in SON magnocellular neurons is pre- or postsynaptic, experiments examining paired-pulse facilitation and mEPSCs were conducted. The results of paired-pulse experiments, where two voltage pulses separated by 50 ms were used to evoke pairs of EPSCs in the cell, showed that the paired-pulse ratio (pulse2/pulse1) was significantly increased (P < 0.05, n = 9 cells) when neurons were exposed to 100 µM dopamine due to a relatively greater reduction in the amplitude of the first EPSC (Fig. 4). Since paired-pulse facilitation is a result of residual calcium in the presynaptic terminal enhancing the amount of transmitter released with the second pulse and therefore the size of the second EPSC, then changes in the paired-pulse ratio suggest a presynaptic locus for dopamine's action.
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A second method to identify the locus of dopamine-dependent inhibition of evoked EPSCs involved examination of mEPSC activity. The frequency of mEPSC events showed a significant and reversible reduction in the presence of 100-200 µM dopamine (Fig. 5A; P < 0.05, n = 5 cells). This change in frequency can be graphically demonstrated as a significant increase in the inter-event interval (585.1 ± 122.6 vs. 1,524 ± 270.4 ms; n = 5 cells; P < 0.01) (Fig. 5B). This effect on mEPSC frequency was not accompanied by a significant change in the mean mEPSC amplitude (11.86 + 0.47 vs. 12.27 + 0.87 pA, n = 5 cells; P > 0.05) (Fig. 5B), further strengthening the case for a presynaptic locus. In addition, analysis of mEPSC kinetic properties indicated that there was no significant difference in either the time to peak or the decay time constant of the events (Fig. 5C; P > 0.05 in both instances). The frequency of mEPSCs was also reduced by 45 ± 11% (n = 4) following treatment of slices with the D4-specific agonist PD 168077 (50 µM), again without a significant change in mEPSC amplitude (paired t-test, P = 0.4179). Therefore, these results, together with the results from paired-pulse experiments, indicate that dopamine exerts its effects in the SON by inhibiting glutamate release from excitatory inputs onto the magnocellular neurons.
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DISCUSSION |
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In the supraoptic nucleus, the frequency and pattern of
magnocellular cell firing is a major determinant of how much
vasopressin and oxytocin are released at the posterior pituitary into
the systemic circulation (Armstrong 1995; Bourque
and Renaud 1991
). Individual magnocellular neurons receive both
excitatory glutamatergic and inhibitory GABAergic inputs to regulate
their excitability. On top of this is another layer of control
influencing the fidelity and magnitude of glutamatergic or GABAergic
neurotransmission. Adenosine, GABA, opioids, oxytocin, and vasopressin
are among the transmitters which inhibit glutamatergic
neurotransmission onto magnocellular neurons via presynaptic mechanisms
(Kombian et al. 1996
, 1997
; Liu et al.
1999b
; Oliet and Poulain 1999
). Adenosine and
GABA also presynaptically inhibit the release of GABA onto
magnocellular neurons (Mouginot et al. 1998
;
Oliet and Poulain 1999
). The overall result is that the
activity of vasopressin and oxytocin neurons is highly regulated via
modulation of excitatory and inhibitory transmitter release from
afferent fibers contacting these neurons (Pittman 1999
).
Here we show that exogenously applied dopamine also inhibits excitatory
neurotransmission onto the SON magnocellular neurons. This occurred in
nearly all neurons tested. This finding is consistent with
immunocytochemical evidence showing dopaminergic fibers having a
diffuse, generalized distribution throughout the SON (van Vulpen et al. 1999). We also show that the receptor subtype
responsible for inhibition of excitatory neurotransmission is the D4
receptor, based on the sensitivity of the response to clozapine and the D4 specific antagonist L750,667 as well as the ability of the D4
receptor-specific agonist PD 168077 to mimic dopamine's activity. This
is also consistent with immunocytochemical evidence, which shows D4
receptors to be highly expressed in the SON (Defagot et al.
1997
). However, the labeling observed by Defagot and colleagues suggests that the magnocellular neurons themselves are labeled with the
D4 antibody, which would argue against our assertion that
dopamine-dependent inhibition of excitatory neurotransmission had a
presynaptic locus. However, without electron microscopic detail to
argue otherwise, D4 receptors could still be located on glutamatergic terminals.
We have demonstrated for the first time a role for D4 receptors in the
SON. Surprisingly very little information has been gathered regarding
the physiology of D4 receptors in the CNS. In cerebellar granule cells,
D4 receptors have been shown to inhibit L-type calcium channels
(Mei et al. 1995). Experiments on D4 receptors in
expression systems have found receptor activation to inhibit voltage-gated potassium channels and activate inwardly rectifying potassium channels (Liu et al. 1999a
; Werner et
al. 1996
). D4 receptors also have been shown to occur
presynaptically in the rat nucleus accumbens shell where they may
mediate the action of dopamine (Svingos et al. 2000
).
With the recent availability of D4 receptor-specific agonists, it is
likely that this paucity in understanding the physiological role for D4
receptors in the CNS should change.
The mechanism through which D4 receptor activation leads to presynaptic
inhibition of evoked EPSCs remains unknown. Modulation of voltage-gated
conductances, to alter either action potential kinetics or calcium
channel properties could, by either mechanism, cause presynaptic
inhibition by reducing calcium entry into the presynaptic terminal.
However, because mEPSC activity has been shown to be largely
independent of calcium entry, it is unlikely that this mechanism is
responsible for dopamine's actions on EPSCs in magnocellular neurons
(Inenaga et al. 1998). There are, however, several
studies that have shown the protein machinery directly responsible for
synaptic vesicle release can also be subject to modulation
(Morishita and Alger 1997
; Trudeau et al.
1996
). Interestingly, there are studies that have reported that
activation of cAMP-dependent kinase can enhance the release of
neurotransmitter (Chen and Regehr 1997
; Kondo and
Marty 1997
). As the classical mechanism of D4 receptor
activation is inhibition of cAMP-dependent kinase, it is tempting to
speculate that this may be the mechanism for inhibition of glutamate
release seen here.
In determining the pharmacological identity of the dopamine receptor,
we took advantage of the relative selectivity of various agonists and
antagonists for the different receptor subtypes. ()Sulpiride and SCH
23390 both have relatively high Ki
values for D4 receptors and both were ineffective as antagonists,
unlike clozapine which shows an approximately 10-fold greater
sensitivity for D4 receptors than either D2 or D3 receptors
(Seeman and Van Tol 1994
). However, less predictable was
the situation for quinpirole, which has a
Ki for D4 receptors similar to that of
dopamine but was a poor mimic at inhibiting evoked EPSCs. Recent
experiments using a cloned rat D4 receptor expressed in hamster CCL39
cells have demonstrated that, whereas quinpirole had an
EC50 similar to that of dopamine for inhibiting
forskolin-dependent cAMP accumulation, quinpirole was deficient
compared with dopamine in its G-protein activation based on stimulation
of [35S]GTP
S binding in CCL39 cells
(Gazi et al. 2000
). Therefore, if the
G-protein-mediating dopamine inhibition of evoked EPSCs were limiting,
then quinpirole would be expected to be less effective than dopamine at
initiating the response.
In several studies to date, dopamine has had a generally excitatory
influence on the release of oxytocin and vasopressin from the SON. In
two reports, one from work in lactating rats and one from experiments
on rat hypothalamic slices, exogenously applied dopamine increased
oxytocin release and the spiking frequency of oxytocinergic neurons
(Mason 1983; Parker and Crowley 1992
). Both of these effects were attributed to a D1-like receptor, which is
also abundantly expressed in the SON (Rivkees and Lachowicz 1997
), but no specific mechanism was found to account for this action. As our experiments were conducted in voltage clamp and examined
specifically the evoked EPSC, we would not detect a parallel effect on
voltage-gated currents, which would influence excitability in the
absence of effects on excitatory neurotransmission. However, in another
study, dopamine depolarized magnocellular neurons via activation of a
nonspecific cation current through a mechanism dependent on D2 receptor
activation (Yang et al. 1991
). We found a small inward
shift in holding current that would have depolarized the magnocellular
neurons to a similar extent to that seen by Yang and colleagues (~4
mV). Moreover, this small inward shift in holding current was inhibited
in the presence of the D2 antagonist sulpiride, supporting the
observation that this was D2-dependent. This leads to the question of
which dopamine response would predominate under physiological
conditions. Unfortunately very little is known about the dynamics of
dopamine release in the SON. Information examining the nature and
control of the dopaminergic input to the SON would go a long way toward
clarifying this issue.
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ACKNOWLEDGMENTS |
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We thank Dr. H. Van Tol for advice on dopamine receptor pharmacology and Dr. M. Hirasawa for comments on the manuscript. Q. J. Pittman is an Alberta Heritage Foundation for Medical Research Heritage Medical Scientist.
This research was supported by the Medical Research Council.
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FOOTNOTES |
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Address for reprint requests: C. J. Price, Dept. of Physiology and Biophysics, University of Calgary, 3330 Hospital Dr., Calgary, Alberta T2N 4N1, Canada (E-mail: cprice{at}ucalgary.ca).
Received 1 November 2000; accepted in final form 16 May 2001.
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REFERENCES |
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