Affiliations of authors: D. S. Michaud (Department of Nutrition), D. Spiegelman (Departments of Epidemiology and Biostatistics), Harvard School of Public Health, Boston, MA; S. K. Clinton, The Arthur G. James Cancer Hospital and Research Institute, Ohio State University, Columbus; E. B. Rimm, W. C. Willett, Departments of Nutrition and Epidemiology, Harvard School of Public Health, and Channing Laboratory, Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Boston; E. L. Giovannucci, Department of Nutrition, Harvard School of Public Health, and Channing Laboratory, Department of Medicine, Harvard Medical School, Brigham and Women's Hospital.
Correspondence to: Dominique S. Michaud, Sc.D., Department of Nutrition, Harvard School of Public Health, Boston, MA 02115 (e-mail: hpdsm{at}gauss.bwh.harvard.edu).
![]() |
ABSTRACT |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
![]() |
INTRODUCTION |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Tobacco smoking is the most clearly established modifiable risk for bladder cancer, but dietary fruits and vegetables, as for many other cancers, may play a preventative role (2). In addition to being a rich source of certain vitamins, minerals, and fiber, these foods are a source of thousands of phytochemicals that may have anticancer properties (3). Proposed mechanisms for the antitumor activity of these phytochemicals are numerous; they include antioxidant properties (4), activation of carcinogen-detoxifying enzymes (5), and inhibition of growth factor stimulation of the tumor cell proliferation (6).
Overall, epidemiologic studies on bladder cancer incidence support a protective effect of fruit and vegetable intake (7-13), although some studies have reported no association (14-17). Previous studies also have been inconsistent with regard to which fruits and vegetables or which subtype of vegetables (e.g., yellow versus green leafy vegetables) may be responsible for an inverse association with bladder cancer. Consequently, it is difficult to determine if the observed relationships represent a cumulative influence of many chemopreventive agents obtained from total fruit and vegetable intake or a more specific effect of a few anticarcinogenic agents obtained from a subset of fruits or vegetables. Whether the putative beneficial effects of fruits or vegetables is modified by cigarette smoking status is also poorly understood.
The purpose of this study was to examine prospectively the relationship between total and type of fruit and vegetable intake and bladder cancer risk in males.
![]() |
SUBJECTS AND METHODS |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
The Health Professionals Follow-up Study is a prospective cohort study initiated in 1986, when 51 529 predominantly white men aged 40-75 years answered a detailed questionnaire by mail on diet and medical history. This cohort consists of dentists (57.6%), veterinarians (19.6%), pharmacists (8.1%), optometrists (7.3%), osteopathic physicians (4.3%), and podiatrists (3.1%). All 50 states of the United States were represented, and no exclusions were made by race. Every 2 years, follow-up questionnaires were mailed to all surviving cohort members, up to six times per follow-up cycle for nonrespondents, to update data on medical conditions and exposures. This investigation was approved by the Human Subjects Committee of the Harvard School of Public Health.
To form the cohort for analysis, we excluded 1596 men with implausibly high or low scores for total food intake (outside the range of 800-4200 kcal/day) or with 70 items or more left blank on the baseline dietary questionnaire and 18 men with missing date of birth. In addition, 2006 men with cancers (excluding nonmelanoma skin cancer) diagnosed before 1986 were excluded because these men may have changed their diets as a result of their disease. The remaining 47 909 men were eligible for follow-up. The follow-up rate for this cohort averaged 94% per follow-up cycle during the five biennial cycles during the period from 1986 through 1996. The National Death Index was used to determine vital status for nonrespondents, and the remaining nonrespondents were assumed to be alive and at risk for bladder cancer.
Dietary Assessment
To assess dietary intake, we used a 131-item semiquantitative food-frequency questionnaire (FFQ) (18), which is an expanded version of a previously validated questionnaire (19). The baseline dietary questionnaire was administered in 1986, and dietary intake information was updated in 1990 and 1994. The questionnaire assesses average frequency of intake over the previous year. For each man, we calculated caloric and nutrient intakes by multiplying the frequency that each food item was reported by the caloric or nutrient content for the specified portion size. Food composition data were primarily based on the nutrient database of the U.S. Department of Agriculture (20). For carotenoid values, we used the new U.S. Department of Agriculture-National Cancer Institute database that was developed for fruits and vegetables and that includes data from a publication on tomato-based food products (21,22).
For the examination of food groups, such as total intake of fruits and vegetables, frequency of consumption was summed across all foods belonging to each group. Because most servings for fruits and vegetables specified on the FFQs are similar in size (e.g., one-half cup or one fruit), we did not standardize intake before summing up frequency of use. The "total vegetable" group consists of the following: string beans; broccoli; sauerkraut; coleslaw and uncooked cabbage; cooked cabbage; cauliflower; Brussels sprouts; carrots; corn; peas or lima beans; mixed vegetables; beans or lentils (baked or dried); alfalfa sprouts; celery; mushrooms (fresh, cooked, or canned); yellow (winter) squash; eggplant, zucchini, or other summer squash; yams or sweet potatoes; spinach (cooked); spinach (raw); kale and mustard or chard greens; iceberg or head lettuce; romaine or leaf lettuce; green pepper; garlic (fresh or powdered); tomatoes; tomato juice; tomato sauce; red chili sauce; and tofu or soybeans. The "total fruit" group consists of the following: raisins; avocado; bananas; cantaloupe; watermelon; fresh apples or pears; apple juice or cider; oranges; orange juice; grapefruit; grapefruit juice; other fruit juices; strawberries; blueberries; and peaches, apricots, or plums.
Fruits and vegetables were also grouped into the following categories: yellow vegetables, consisting of carrots, yams, sweet potatoes, and yellow squash; green leafy vegetables, consisting of spinach (raw and cooked), kale (mustard and chard greens), and romaine and leaf lettuce; cruciferous vegetables (Brassica oleracea family), consisting of broccoli, cabbage, cauliflower, Brussels sprouts, coleslaw, sauerkraut, and kale (note that the "kale" item on the questionnaire also includes mustard and chard greens that do not belong to the cruciferous B. oleracea family); and citrus fruits, consisting of oranges, orange juice, grapefruit, and grapefruit juice. Similar groupings have been reported previously in epidemiologic studies (17,23) examining associations between fruit and vegetable intakes and cancer outcomes and represent, to some extent, foods rich in certain phytochemicals that may influence cancer risk (i.e., yellow vegetables are high in ß-carotene, green leafy vegetables are high in lutein, cruciferous vegetables are high in glucosinolates, and citrus fruits are high in vitamin C).
A reproducibility and validity study of the FFQ among 127 men in this cohort indicated that
most foods are reported reasonably well; Pearson correlations between the average intake
assessed by two 1-week diet records completed 6 months apart and 1-FFQ (completed after the
diet records) ranged from 0.25 to 0.95 for specific fruits and vegetables, and the median
correlations for fruits and vegetables were .77 and .46, respectively (24).
Correlations between the two diet records and the 1-FFQ for fruit and vegetable groupings used
in this study were calculated by use of data from the validation study and were as follows: .37 for
cruciferous vegetables, .40 for vegetables and yellow vegetables, .45 for green leafy vegetables,
.61 for total fruits and vegetables, .78 for fruits, and .87 for citrus fruits. Intakes of carotenoids
(-carotene, ß-carotene, lutein, lycopene, and ß-cryptoxanthin) were reported
previously to correlate reasonably well with specific carotenoid plasma levels in a subset of men
from this cohort (correlations ranged between .35 and .47) (25).
Assessment of Nondietary Factors
At baseline and biennially thereafter, men provided information on their state of residence, current cigarette smoking status, exercise habits, weight, height, and medication use. The baseline questionnaire provided detailed information on past smoking habits, time since quitting, and average number of cigarettes smoked per day before age 15 and at ages 15-19 years, 20-29 years, 30-39 years, 40-49 years, 50-59 years, and 60 years and older. To control for smoking, total pack-years of smoking was derived to incorporate all past smoking experience. One cigarette pack-year is equivalent to having smoked one pack or 20 cigarettes per day for an entire year.
Case Ascertainment
On each questionnaire, participants indicated whether they had been diagnosed with any major cancer (e.g., prostate or colon cancer), heart disease, or other medical conditions. Participants were required to write in "bladder" in a space provided for "other cancers." We confirmed the self-reported diagnosis of bladder cancer by reviewing medical records. When denied permission to obtain medical records, we attempted to confirm the initial cancer report and date of diagnosis with an additional letter or telephone call. If the primary cause of death, reported by the National Death Index, was a previously unreported bladder cancer, we contacted family members to obtain permission to retrieve medical records or, at the very least, to confirm the diagnosis of bladder cancer. For three bladder cancer cases, we used death certificates to confirm a previous, unconfirmed self-reported diagnosis of bladder cancer.
The end points in this study were 252 bladder cancer cases newly diagnosed during the period from 1986 through January 31, 1996, of which 213 were confirmed with medical records. Unconfirmed cases were kept in the analyses after verifying that similar results were obtained when those cases were excluded. On the basis of review of pathology reports, more than 90% of bladder cancer cases were transitional cell carcinomas.
Statistical Analysis
We computed person-time of follow-up for each participant from the return date of the 1986 questionnaire to the date of bladder cancer diagnosis, to the day of death from any cause, or January 31, 1996, whichever came first. In the main analysis, exposure categories were determined by the 1986 questionnaire (except for age and current smoking status, which were updated every 2 years in all analyses). The incidence rate for each category of fruit and vegetable intake was calculated as the number of cases with bladder cancer divided by the person-time of follow-up. Cut points for the different groupings of fruit and/or vegetable intakes were obtained by dividing each into five even categories (i.e., quintiles) and then finding the closest whole (or half) frequency of use for each cutoff. By doing this, we created categories of intake that can be easily interpreted. To adjust for age (5-year categories), pack-years of smoking, and current smoking status (updated every 2 years), we used pooled logistic regression (26). This approach is asymptotically equivalent to the Cox regression model with time-dependent covariates, given short time intervals and low probability of the outcome within the interval (26). In multivariate (MV) analyses, in addition to age and pack-years of smoking, we adjusted for geographic region and fluid intake because both of these variables were risk factors in this population. U.S. states were grouped into five geographic regions, according to the U.S. Census definitions: West, Midwest, South, Northeast, and Pacific. Total caloric intake was also included in MV models to minimize extraneous variation introduced by underreporting or overreporting in the FFQ (27). Because this cohort consists of health professionals, the socioeconomic status of the study members is sufficiently homogeneous not to have to control for this variable.
In a separate analysis, we examined the relationship between total and type of fruit and vegetable intakes and bladder cancer incidence by updating the baseline food groups with diet from subsequent questionnaires (in 1990 and 1994). In these analyses, dietary data from the 1986 questionnaire were used to predict outcomes during the period from 1986 to 1990, the average of 1986 and 1990 dietary intakes was used to predict outcomes during the period from 1990 to 1994, and the average of 1986, 1990, and 1994 was used for subsequent cases (i.e., 1994 to 1996). Cumulative averaging may reduce within-person variation and better represent long-term intake. Tests for trend were obtained by assigning the median value for each category and modeling this variable as a continuous variable, using pooled logistic regression for MV analyses. Tests for statistical (multiplicative) interaction were performed by use of likelihood ratio tests.
![]() |
RESULTS |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Total fruit and vegetable intake was not significantly associated with the risk of bladder
cancer after adjustment was made for pack-years of smoking, current smoking status, age,
geographic region in the United States, and total fluid and caloric intake (two-sided P value, test for trend = .25; Table 1). The MV RR of bladder
cancer incidence for the top category of total fruit and vegetable intake (>8.0 servings/day),
compared with the bottom category (<3.5 servings/day) was 0.75 (95% CI =
0.49-1.14). Although the age-adjusted association between total fruit and vegetable intake and
bladder cancer risk was significant before adjustment for other variables (P for trend
= .04), pack-years of smoking was inversely associated with fruit and vegetable
consumption and accounted for all the differences between the age-adjusted and the MV
analyses. Thus, when we included only age, pack-years of smoking, current smoking status, and
total fruit and vegetables in a separate model, the RR for the highest quintile of total fruit and
vegetable intake compared with the lowest was identical to that from the full MV model (RR
= 0.75; 95% CI = 0.51-1.10).
|
In an age-adjusted model, we observed a 51% risk reduction of bladder cancer among
those consuming a high intake (>5.0 servings/week) compared with a low intake of
cruciferous vegetables (1.0 serving/week; Table 1
). The inverse
association for cruciferous vegetable intake and bladder cancer risk persisted after adjustment for
potential confounders and remained statistically significant (P for trend = .008).
When we modeled cruciferous vegetables and total vegetables simultaneously using the same
categories as those used in Table 1
, the highest intake of cruciferous
vegetable (
5.0 servings/week) compared with the lowest (
1.0 serving/week) was
statistically significant (MV RR = 0.57; 95% CI = 0.36-0.93), whereas the
RR for the top category (>8.0 servings/day) versus the bottom category (<3.5 servings/day)
of total vegetable intake was not significant (MV RR = 0.83; 95% CI =
0.52-1.35). On average, cruciferous vegetables account for 14% of total vegetable
consumption in this cohort.
In an additional analysis, we divided men according to tertiles of cruciferous vegetable intake
and tertiles of total vegetable intake and examined the relationship between cruciferous vegetable
intake and bladder cancer risk at different levels of total vegetable intake (Table 2). Compared with low cruciferous and low total vegetable intake, high intake of
cruciferous vegetables was associated with a lower risk of bladder cancer in each of the tertiles of
total vegetable intake. In contrast, total vegetable intake was not strongly inversely associated
with bladder cancer risk in the lower tertiles of cruciferous vegetable intake. The RRs were
somewhat attenuated compared with the quintile analysis because the range of intake was
narrower in the tertile analysis. The median intake of cruciferous vegetables in the high
cruciferous/high total vegetable intake category was 6.5/week compared with 4.4/week for the
high cruciferous/low vegetable intake category, probably explaining the slight inverse trend
across the tertiles of total vegetable intake in the top cruciferous vegetable tertile. Therefore, the
inverse association observed between cruciferous vegetable intake and bladder cancer risk is
independent of an increase in total vegetables and may be explained by one or more
phytochemical(s) specific to those vegetables.
|
|
|
|
To obtain a measure of vegetable intake representative of very long term intake, we restricted the analyses to those individuals who reported having had the same vegetable intake "over the past 10 years" on the 1986 questionnaire. One hundred fifty-four cases were available for these analyses. MV RRs for cruciferous vegetable intake and bladder cancer risk were very similar to those observed in the main analyses (top versus bottom category comparison MV RR = 0.47; 95% CI = 0.25-0.87). In the fruit and vegetable analysis, the association with bladder cancer risk was weaker than in the main analysis (top versus bottom category comparison MV RR = 1.06; 95% CI = 0.62-1.82).
We also examined each 15 individual fruit and 30 vegetable items present on the baseline questionnaire. Excluding cruciferous vegetables, no individual vegetable or fruit item was statistically associated with bladder cancer risk in MV models. In addition, only three of the 30 vegetable items besides broccoli, cabbage, cauliflower, Brussels sprouts, and kale had MV RRs below 0.80 for the top versus the bottom category of intake; these vegetables were cooked spinach, yams, and yellow squash, and associations were not statistically significant.
Because carotenoids are found in various fruits and vegetables, have antioxidant properties,
and have been shown to have an anticarcinogenic effect on bladder cancer in experimental
studies (28), we examined the relationship between the dietary intake of
five major carotenoids and bladder cancer risk. In this study, we observed no association between
intake of lutein, lycopene, -carotene, ßcarotene, or ß-cryptoxanthin and risk of
bladder cancer (Table 6).
|
![]() |
DISCUSSION |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
In epidemiologic studies, null associations can arise in situations where the range of exposure
is too small and there is insufficient power to detect an association. As shown in Table 1, the contrast between high and low consumption (difference between
high and low medians) was far greater for total vegetable intake (4.8 servings/day) than for
cruciferous vegetable intake (approximately 1 serving/day). For yellow, green leafy, and
cruciferous vegetables, the contrasts were similar (approximately 1 serving/day). Therefore, it is
unlikely that the null associations for groupings other than cruciferous vegetables were a result of
a limited range of intake or misclassification.
Dietary assessment methods used in previous studies have often not been sufficiently comprehensive to estimate total fruit or vegetable intake. Only three case-control studies (14,29,30) and two cohort studies (13,17) have had data available to evaluate the relationship between total fruit or vegetable intake and bladder cancer risk. Total fruit intake (30) and total fruit and vegetable intake in men only (29) were inversely related to bladder cancer risk in two of the three case-control studies. Both cohort studies observed an inverse association for total fruit and bladder cancer risk with the use of 24-hour recalls (13) and FFQs (17); however, no association was reported for total fruit and vegetable intake [(17); vegetables were not measured in (13)].
To our knowledge, only one study (7), published in 1979, previously examined the association between cruciferous vegetable intake per se and bladder cancer risk. The reported association for that study was inverse (RR = 0.75 for high versus low consumption) but not statistically significant. Two additional studies (8,9) have found statistically significant inverse associations between dark green vegetable consumption and bladder cancer risk and, although one study did not specify the individual vegetables comprising "dark green vegetables" (8), broccoli was included in the other (9). One cohort study of 71 cases (17) did not report an association between "dark green vegetable" intake (other leafy greens, broccoli, and Brussels sprouts) and bladder cancer risk.
Cruciferous vegetables of the genus and species Brassica oleracea, which include broccoli, cabbage, cauliflower, Brussels sprouts, kale, collard, and kohlrabi, have been widely studied for their anticarcinogenic properties in experimental studies (31-33) and have been associated with reduced cancer risk (16,34). Consumption of cruciferous vegetables induces detoxification enzymes in animal tissues (35-37) and stimulates metabolism of drugs and other xenobiotics in humans (38,39). Extracts of cruciferous vegetables have led to the identification of numerous compounds, e.g., isothiocyanate sulforaphane (40), that are capable of inducing phase 2 detoxifying enzymes in vitro. Therefore, one possible mechanism whereby an increase in cruciferous vegetable intake protects the bladder epithelium from carcinogens is by enhancing detoxification of xenobiotics prior to excretion, although other mechanisms are plausible.
In a stratified analysis, we observed a strong inverse association between cruciferous vegetable intake and bladder cancer in nonsmokers and weak nonsignificant inverse associations in past and current smokers. The inverse association in nonsmokers indicates that residual confounding by smoking is unlikely to be responsible for the overall inverse association observed between cruciferous vegetable intake and risk of bladder cancer, yet, because smokers are constantly metabolizing and excreting carcinogens obtained from smoke (41), we would have expected them to benefit the most from faster metabolism of carcinogens (assuming that phytochemicals in cruciferous vegetables are able to activate detoxifying enzymes). Although the RRs related to cruciferous vegetable consumption were stronger among nonsmokers, the test for statistical multiplicative interaction was not statistically significant. More data are needed to determine whether smoking status modifies the inverse association between cruciferous vegetable intake and risk of bladder cancer.
Of the cruciferous vegetables, broccoli and cabbage were independently related to the risk of bladder cancer in this study. Increasing broccoli intake was associated with decreasing risk of bladder cancer in each stratum of cabbage intake, and a similar pattern was observed for cabbage across broccoli intake. After we controlled for broccoli intake, cauliflower intake was no longer significantly associated with bladder cancer risk. Although coleslaw and sauerkraut are made from cabbage, neither of these two vegetables was associated with bladder cancer risk, even before adjustment for broccoli intake. Measurement error could explain some of these differences if some vegetables are reported with less accuracy than others, resulting in attenuated associations in the poorly measured vegetables. This could explain the lack of association for coleslaw and sauerkraut intake [our validation study reported a deattenuated correlation between an FFQ and dietary records of .37 for coleslaw and an observed correlation of .21 for sauerkraut (24)] but not for Brussels sprouts intake, inasmuch as our validation study indicates that it is measured with similar accuracy as broccoli [deattenuated correlations: .51 for Brussels sprouts compared with .46 for broccoli (24)]. Because the ranges of intake for individual cruciferous vegetables were similar, with the exception of broccoli intake, which had a wider range, associations are not likely to be the result of power differences. Alternatively, pickling of cabbage to make sauerkraut may destroy important phytochemicals or create undesired ones (42) and influence the relation to bladder cancer risk.
We cannot rule out the possibility that findings from this study are a consequence of multiple testing. We performed more than 50 tests (including tests on individual fruits and vegetables), thus increasing the probability of finding a significant result by "chance" with each additional test. However, given that the vegetables with significant associations were from a common family (cruciferae) and that there is plausible biologic evidence to support this finding, it is unlikely that our results were chance findings.
We did not observe a relationship between yellow vegetable intake or carrot intake alone and bladder cancer risk. Two case-control studies (7,8) have reported a significant inverse association between carrot intake and bladder cancer risk, but two cohort studies (16,17) reported no association between yellow vegetable intake and bladder cancer risk. Carrots have often been associated with the risk of various cancers (43) and are of interest because of their high carotenoid content. We therefore examined the association between five important carotenoids and bladder cancer risk, but we observed no relationship for any of those carotenoids.
In this study, diet was determined before cancer diagnosis, with a time interval from 1 month to 10 years. Although we do not know at which point fruit and vegetable intake may influence bladder carcinogenesis, it is possible that, with longer follow-up, we would have observed different associations. However, restricting the analyses to men who had indicated in 1986 that their consumption of fruits and vegetables had not changed during the past 10 years did not affect the associations. We also repeated all of our analyses using cumulative averages of diet with updating of the 1990 and 1994 diet questionnaires to reduce within-person variation over the follow-up period. Since the associations in the restricted and in the updated analyses were similar to the main analyses, it is unlikely that a longer follow-up period would significantly alter our results.
More studies are needed to confirm findings from this study in men as well as in women. Because bladder cancer incidence rates are three to four times greater in men than in women, associations observed in men may not necessarily be generalized to women. Biologic responses to certain carcinogens may well vary by sex; thus, chemopreventive agents may be equally affected.
In this prospective study, we report a strong inverse association between the intake of cruciferous vegetables and bladder cancer risk. However, we did not observe a statistically significant relationship between total fruit and vegetable intake and incidence of bladder cancer after we controlled for potential confounders such as smoking status. The inverse association between cruciferous vegetable intake and bladder cancer risk was strongest in never smokers. Although confirmation in other prospective studies is required, data from this study suggest that high intake of cruciferous vegetables, such as broccoli, may substantially reduce bladder cancer risk.
![]() |
NOTES |
---|
![]() |
REFERENCES |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
1 Ries LA, Miller BA, Hankey BF, Kosary CL, Harras A, Edwards BK, editors. SEER cancer statistics review, 1973-1997: tables and graphs. Bethesda (MD): National Institutes of Health, National Cancer Institute; 1997 Report No.: DHHS Publ No. (NIH)97-2789.
2 Cohen SM, Johansson SL. Epidemiology and etiology of bladder cancer. Urol Clin North Am 1992;19:421-8.[Medline]
3 Steinmetz KA, Potter JD. Vegetables, fruit, and cancer. II. Mechanisms. Cancer Causes Control 1991;2:427-42.[Medline]
4 Mathews-Roth MM. Antitumor activity of ß-carotene, canthaxanthin and phytoene. Oncology 1982;39:33-7.[Medline]
5 Beecher CW. Cancer preventive properties of varieties of Brassica oleracea: a review. Am J Clin Nutr 1994;59(5 suppl):1166S-1170S.[Abstract]
6 Adlercreutz CH, Goldin BR, Gorbach SL, Hockerstedt KA, Watanabe S, Hamalainen EK, et al. Soybean phytoestrogen intake and cancer risk [published erratum appears in J Nutr 1995;125:1960]. J Nutr 1995;125(3 Suppl):757S-770S.[Medline]
7 Mettlin C, Graham S. Dietary risk factors in human bladder cancer. Am J Epidemiol 1979;110:255-63.[Abstract]
8 La Vecchia C, Negri E, Decarli A, D'Avanzo B, Liberati C, Franceschi S. Dietary factors in the risk of bladder cancer. Nutr Cancer 1989;12:93-101.[Medline]
9 Nomura AM, Kolonel LN, Hankin JH, Yoshizawa CN. Dietary factors in cancer of the lower urinary tract. Int J Cancer 1991;48:199-205.[Medline]
10 Vena JE, Graham S, Freudenheim J, Marshall J, Zielezny M, Swanson M, et al. Diet in the epidemiology of bladder cancer in western New York. Nutr Cancer 1992;18:255-64.[Medline]
11 Momas I, Daures JP, Festy B, Bontoux J, Gremy F. Relative importance of risk factors in bladder carcinogenesis: some new results about Mediterranean habits. Cancer Causes Control 1994;5:326-32.[Medline]
12 Mills PK, Beeson WL, Phillips RL, Fraser GE. Bladder cancer in a low risk population: results from the Adventist Health Study. Am J Epidemiol 1991;133:230-9.[Abstract]
13 Chyou PH, Nomura AM, Stemmermann GN. A prospective study of diet, smoking, and lower urinary tract cancer. Ann Epidemiol 1993;3:211-6.[Medline]
14 Riboli E, Gonzalez CA, Lopez-Abente G, Errezola M, Izarzugaza I, Escolar A, et al. Diet and bladder cancer in Spain: a multi-centre case-control study.Int J Cancer 1991;49:214-9.[Medline]
15 Steineck G, Norell SE, Feychting M. Diet, tobacco and urothelial cancer. A 14-year follow-up of 16,477 subjects. Acta Oncol 1988;27:323-7.[Medline]
16 Hirayama T. A large scale cohort study on cancer risks by dietwith special reference to the risk reducing effects of green-yellow vegetable consumption. Princess Takamatsu Symp 1986;16:41-53.
17 Shibata A, Paganini-Hill A, Ross RK, Henderson BE. Intake of vegetables, fruits, beta-carotene, vitamin C and vitamin supplements and cancer incidence among the elderly: a prospective study. Br J Cancer 1992;66:673-9.[Medline]
18 Rimm EB, Giovannucci EL, Stampfer MJ, Colditz GA, Litin LB, Willett WC. Reproducibility and validity of an expanded self-administered semiquantitative food frequency questionnaire among male health professionals. Am J Epidemiol 1992;135:1114-26.[Abstract]
19 Willett WC, Sampson LS, Stampfer MJ, Rosner B, Bain C, Witschi J, et al. Reproducibility and validity of a semiquantitative food frequency questionnaire. Am J Epidemiol 1985;122:51-65.[Abstract]
20 U.S. Department of Agriculture, Agricultural Research Service. USDA nutrient database for standard reference, release 10. Washington (DC): U.S. Department of Agriculture; 1995.
21 Chug-Ahuja JK, Holden JM, Forman MR, Mangels AR, Beecher GR, Lanza E. The development and application of a carotenoid database for fruits, vegetables, and selected multicomponent foods. J Am Diet Assoc 1993;93:318-23.[Medline]
22 Tonucci LH, Holden JM, Beecher GR, Khachik F, Davis CS, Mulokozi G. Carotenoid content of thermally processed tomato-based food products. J Agric Food Chem 1995;19:579-86.
23 Steinmetz KA, Potter JD, Folsom AR. Vegetables, fruit, and lung cancer in the Iowa Women's Health Study. Cancer Res 1993;53:536-43.[Abstract]
24 Feskanich D, Rimm EB, Giovannucci EL, Colditz GA, Stampfer MJ, Litin LB, et al. Reproducibility and validity of food intake measurements from a semiquantitative food frequency questionnaire. J Am Diet Assoc 1993; 93:790-6.[Medline]
25 Michaud DS, Giovannucci EL, Ascherio A, Rimm EB, Forman MR, Sampson L, et al. Associations of plasma carotenoid concentrations and dietary intake of specific carotenoids in samples of two prospective cohort studies using a new carotenoid database. Cancer Epidemiol Biomarkers Prev 1998;7:283-90.[Abstract]
26 D'Agostino RB, Lee ML, Belanger AJ, Cupples LA, Anderson K, Kannel WB. Relation of pooled logistic regression to time dependent Cox regression analysis: the Framingham Heart Study. Stat Med 1990;9:1501-15.[Medline]
27 Willett W. Nutritional epidemiology. New York (NY): Oxford University Press; 1990.
28 Mathews-Roth MM, Lausen N, Drouin G, Richter A, Krinsky NI. Effects of carotenoid administration on bladder cancer prevention. Oncology 1991;48:177-9.[Medline]
29 Claude J, Kunze E, Frentzel-Beyme R, Paczkowski K, Schneider J, Schubert H. Life-style and occupational risk factors in cancer of the lower urinary tract. Am J Epidemiol 1986;124:578-89.[Abstract]
30 Bruemmer B, White E, Vaughan TL, Cheney CL. Nutrient intake in relation to bladder cancer among middle-aged men and women. Am J Epidemiol 1996;144:485-95.[Abstract]
31 Stoewsand GS, Babish JB, Wimberley HC. Inhibition of hepatic toxicities from polybrominated biphenyls and aflatoxin B in rats fed cauliflower. J Environ Pathol Toxicol 1978;2:399-406.[Medline]
32
Boyd JN, Babish JG, Stoewsand GS. Modification by beet and
cabbage diets of aflatoxin B1-induced rat plasma -fetoprotein elevation, hepatic
tumorigenesis and mutagenicity of urine. Food Chem Toxicol 1982;20:47-54.[Medline]
33 Wattenberg LW. Inhibition of neoplasia by minor dietary constituents. Cancer Res 1983;43(5 Suppl):2448s-2453s.[Abstract]
34 Graham S, Dayal H, Swanson M, Mittelman A, Wilkinson G. Diet in the epidemiology of cancer of the colon and rectum. J Natl Cancer Inst 1978;61:709-14.[Medline]
35 Sparnins VL, Venegas PL, Wattenberg LW. Glutathione S-transferase activity: enhancement by compounds inhibiting chemical carcinogenesis and by dietary constituents. J Natl Cancer Inst 1982;68:493-6.[Medline]
36 Aspry KE, Bjeldanes LF. Effects of dietary broccoli and butylated hydroxyanisole on liver-mediated metabolism of benzo[a]pyrene. Food Chem Toxicol 1983;21:133-42.[Medline]
37 Bradfield CA, Chang Y, Bjeldanes LF. Effects of commonly consumed vegetables on hepatic xenobiotic-metabolizing enzymes in the mouse. Food Chem Toxicol 1985;23:899-904.[Medline]
38 Pantuck EJ, Hsaio KC, Loub WD, Wattenberg LW, Kuntzman R, Conney AH. Stimulatory effect of vegetables on intestinal drug metabolism in the rat. J Pharmacol Exp Ther 1976;198:278-83.[Abstract]
39 Pantuck EJ, Pantuck CB, Garland WA, Min BH, Wattenberg LW, Anderson KE, et al. Stimulatory effect of brussel sprouts and cabbage on human drug metabolism. Clin Pharmacol Ther 1979;25:88-95.[Medline]
40
Zhang Y, Talalay P, Cho CG, Posner GH. A major inducer of
anticarcinogenic protective enzymes of broccoli: isolation and elucidation of structure. Proc Natl Acad Sci U S A 1992;89:2399-403.
41 Vineis P. Epidemiological models of carcinogenesis: the example of bladder cancer. Cancer Epidemiol Biomarkers Prev 1992;1:149-53.[Abstract]
42 Jorgen WMF. Glucosinolates in Brassica: occurrence and significance as cancer-modulating agents. Proc Nutr Soc 1996;55:433-46.[Medline]
43 Potter JD, Steinmetz K. Vegetables, fruit and phytoestrogens as preventive agents. IARC Sci Publ 1996;139:61-90.[Medline]
Manuscript received September 14, 1998; revised January 21, 1999; accepted January 28, 1999.
This article has been cited by other articles in HighWire Press-hosted journals:
![]() |
||||
|
Oxford University Press Privacy Policy and Legal Statement |