CORRESPONDENCE

Erratum

"High Frequency of Somatic Frameshift BHD Gene Mutations in Birt-Hogg-Dubé–Associated Renal Tumors" by Vocke et al. [J Natl Cancer Inst 2005;97:931–5 (Issue 12)]. In the print version of the Journal, Figs. 1 and 2 were incorrectly reproduced in black and white. The color versions appear below. The Journal regrets the error.



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Fig. 1. Sequence chromatograms of DNA and clones derived from Birt-Hogg-Dubé tumors. A) Tumor DNA and clones derived from tumor 8L from patient 1. The top panel depicts the wild type sequence in exon 9. The second panel illustrates DNA directly amplified from tumor 8L. The third panel shows a clone with the IVS9+2T>G germline mutation. The bottom panel depicts a clone containing the somatic 2-bp CA deletion (c.1508–1509delCA) 10 nucleotides upstream, but not the germline mutation. B) Tumor DNA and clones derived from tumor 9R from patient 5. The top panel shows the wild-type sequence in the C8 tract region of exon 11. The second panel illustrates DNA directly amplified from tumor 9R. The third panel depicts a clone with the germline mutation with a C9 tract (c.1733insC). The bottom panel shows a clone containing the somatic mutation (c.1742insA) but not the germline mutation. C) LOH demonstrated by sequence chromatograms from direct PCR amplification of BHD tumors. The antisense DNA sequence of a portion of exon 11 is shown. The top panel shows the wild-type sequence from a control DNA sample. The second panel illustrates the normal DNA control from patient 5, showing the overlapping sequences that result from one wild-type copy of BHD and one copy containing the c.1733insC germline mutation. The third panel shows the chromatogram for tumor 1L: only the sequence representing the germline mutation is apparent (note the presence of 9 G peaks). The bottom panel depicts tumor 12R from patient 5; the sequence pattern is similar to tumor 1L.

 


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Fig. 2. Schematic presentation of tumors and mutations found in the kidneys of patient 4. Of 18 tumors examined, 15 tumors, 11 from the left kidney and four from the right kidney, exhibited a somatic second hit. Tumors with two colors depict those in which areas of different histologic type were microdissected separately from frozen sections; these tumors are categorized from formalin-fixed sections as oncocytic hybrid tumors. Mutations or LOH were observed in all histologic type and in both kidneys.

 


             
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