Affiliations of authors: G. D. Kirk, International Agency for Research on Cancer, Banjul, The Gambia, and Lyon, France, and Viral Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD; A.-M. Camus-Randon, P. Hainaut, R. Montesano, International Agency for Research on Cancer, Banjul and Lyon; M. Mendy, Medical Research Council Laboratories, Banjul; J. J. Goedert, Viral Epidemiology Branch, National Cancer Institute; P. Merle, C. Trépo, INSERM Unité 271, Lyon; C. Bréchot, Hepatology Unit, Necker Hospital, Paris, France.
Correspondence to: Ruggero Montesano, M.D., Ph.D., Unit of Mechanisms of Carcinogenesis, International Agency for Research on Cancer, 150 cours Albert-Thomas, 69372 Lyon Cedex 08, France (e-mail: montesano{at}iarc.fr).
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ABSTRACT |
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INTRODUCTION |
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Tumor-specific p53 mutations have been identified in several human cancers (7,8). A selective guanine-to-thymine transversion mutation in codon 249 (AGG to AGT [transversion underlined] leading to an arginine-to-serine substitution) of the p53 gene (also known as TP53) has been identified as a "hotspot" mutation for HCC (9,10). Epidemiologic evidence and experimental evidence have suggested that in HCC this mutation is strongly associated with exposure to AFB1 (6,7,11).
The Gambia in West Africa has a population with a high incidence of HCC, a high level of endemic, chronic HBV infection, and a high exposure to AFB1. Data from the Gambian National Cancer Registry indicate that HCC is the most common cancer in males and the second most common cancer in females (12,13). Ten percent to 20% of the Gambian population is chronically infected with HBV (14,15). Extensive analysis of individual biomarkers of aflatoxin exposure in The Gambia has demonstrated ubiquitous dietary exposure, with more than 95% of the population having detectable levels of aflatoxin-albumin adducts in their serum (6,11).
Recent research has shown that DNA can be isolated from the plasma or serum of patients with cancer; this plasma DNA carries the same genetic mutations as DNA in the tumor. Thus, plasma DNA can be used as surrogate material to detect genetic alterations present in the original tumor (16-18). We have used this method to determine whether Ser-249 p53 mutations can be detected in plasma DNA isolated from Gambian patients with HCC, Gambian patients with cirrhosis, and healthy Gambian control subjects, as well as from European patients with HCC or with cirrhosis.
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MATERIALS AND METHODS |
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Incident cases of HCC and cirrhosis were identified from liver disease referral clinics at each of the three tertiary referral hospitals in The Gambia (i.e., Royal Victoria Hospital, Medical Research Council Hospital, and Bansang Hospital). Samples from The Gambia analyzed in this study were from subjects sequentially recruited from September 1997 through June 1998 into an ongoing case-control study of HCC. We amplified DNA from 142 (92%) of 155 subjects enrolled during this period; we could not amplify DNA from 13 subjects. Evaluation of subjects included a clinical examination, ultrasonography, collection of biologic specimens, and a structured interview.
For inclusion in the study, a patient with HCC had to have compatible clinical and
ultrasonographic findings and serum -fetoprotein levels of 100 ng/mL or more.
Twenty-three patients with HCC, who were initially recruited into the study, were excluded
because their levels of
-fetoprotein were less than 100 ng/mL. Analyses performed with and
without inclusion of these subjects were similar. The results presented include only patients who
met the case definition of HCC, for a total of 119 study subjects (53 patients with HCC, 13
patients with cirrhosis, and 53 control subjects). Patients with cirrhosis were included as an
additional referent group, in addition to healthy control subjects, to evaluate factors associated
with the progression to HCC. The diagnosis of cirrhosis was based on compatible clinical history
and ultrasonographic patterns characteristic of cirrhosis, without any focal lesions suggestive of
HCC. A minority of patients with liver disease also had histologic confirmation. Control subjects
were individuals with no history or clinical findings suggestive of liver disease, who were
recruited from the outpatient clinics of one of the three tertiary referral hospitals. Control subjects
were frequency matched to case patients with HCC by age (within 10-year groupings), sex, and
recruitment site. Local and international ethical and scientific review committees approved the
study protocol, and informed consent was obtained from each participant before inclusion in the
study.
To compare West African subjects with subjects from areas with lower exposure to aflatoxin and HBV, we evaluated plasma and serum samples obtained from 60 non-African patients from Europe (Hotel Dieu Hospital [Lyon, France] and Necker Hospital [Paris, France]). Subjects included 50 patients with HCC (21 associated with HBV, 17 with hepatitis C virus, seven with alcohol, and five with unknown status) and 10 patients with cirrhosis (three associated with HBV, four with hepatitis C virus, and seven with alcohol [some patients with more than one association]). The samples were sent to the laboratory of the International Agency for Research on Cancer (IARC) and were analyzed in the same manner as the samples from The Gambia.
DNA Extraction and Polymerase Chain Reaction
Blood specimens anticoagulated with EDTA were processed immediately after collection and
stored at -70 °C at the Medical Research Council Laboratories, The Gambia, until
subsequent testing. -Fetoprotein and HBV serologic testing was performed with standard
laboratory kits. A 500-µL aliquot of plasma was shipped on dry ice to IARC laboratories
(Lyon, France) to test for the Ser-249 p53 mutation. DNA was extracted from 200 µL of
plasma with a QiAmp tissue kit (Qiagen, Hilden, Germany). The purified DNA was eluted from
the QuiAmp silica column with one volume (50 µL) of nuclease-free water (Promega Corp.,
Madison, WI).
Ten microliters of DNA eluate was used to amplify exon 7 of the p53 gene with primers located in the introns flanking exon 7, as described by Lehman et al. (19) The following primers (Genset, Paris, France) were used: p1 (sense), 5'-CTTGCCACAGGTCTCCCCAA-3'; p2 (antisense), 5'-AGGGGTCAGCGGCAAGCAGA-3'.
The 50-µL reaction mixture contained 50 mM KCl, 20 mM Tris-HCl (pH 8.4), 1.5 mM MgCl2 , all four deoxynucleoside triphosphates (each at 0.2 mM; Promega Corp.), 0.2 µM of each primer, and 2.5 U of Platinum Taq DNA polymerase (Life Technologies, Inc. [GIBCO BRL], Gaithersburg, MD). After an incubation (94 °C for 2 minutes), 35 cycles (94 °C for 30 seconds, 60 °C for 30 seconds, and 72 °C for 30 seconds) were performed and were followed by a final 5-minute extension at 72 °C in a thermal cycler (gene Amp PCR system, 9600; The Perkin-Elmer Corp., Foster City, CA). The amplification products (254 base pairs [bp]) were visualized by staining with ethidium bromide after electrophoresis on 3% agarose gel.
In six samples, the polymerase chain reaction (PCR) products were not clearly detected, and a second amplification reaction was performed with the following nested primers: p3 (sense), 5'-AGGCGCACTGGCCTCCTT-3'; p4 (antisense), 5'-TGTGCAGGGTGGCAAGTGGC-3'. This reaction mixture contained 5 µL of the first PCR products, and 25 cycles (as in the first PCR) were performed. The same nested PCR protocol was also used to generate enough material for DNA sequence analysis (see below). All sets of PCRs included negative control samples (containing no template DNA). For avoidance of PCR contaminant artifacts, routine precautions included performing DNA extraction, PCR assembly, and the PCR in separate rooms and using barrier tips at all stages of the procedure.
Mutation Detection by Restriction Endonuclease Analysis
PCR products (10 µL) were digested with restriction endonuclease HaeIII
(Boehringer Mannheim GmbH, Mannheim, Germany), according to the manufacturer's
instructions. This enzyme cleaves a GG/CC sequence between codon 249 and codon 250 to
generate two fragments of 92 bp and 66 bp (plus several small fragments) from the 254-bp
product of the first PCR. The presence of a mutation in codon 249 or codon 250 results in an
uncleaved 158-bp fragment. These fragments were separated and identified on 3% agarose
gel stained with ethidium bromide (Fig. 1). The absence of the 254-bp
band (full-length PCR products) provides a control for complete digestion of the PCR product.
Restriction endonuclease assays included both positive (DNA with a Ser-249 p53 mutation
isolated from a patient with liver cancer) and negative (wild-type DNA) controls. All reactions
were carried out in duplicate. As a further control, five positive and five negative assays were
selected at random and submitted to a second, independent analysis, including DNA extracts,
PCR, and restriction endonuclease digestion. Concordant results were obtained in each case.
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The 158-bp product, not cleaved between codon 249 and codon 250, was excised from
agarose gels and further amplified with nested PCR primers P3 and P4, as described above. The
products of this second PCR were purified with QIA quick-spin columns (Qiagen), and 0.25
µg of DNA (exon 7, codons 225-260) was analyzed by automated DNA sequencing
(sequencer 377; The Perkin-Elmer Corp.) with the use of the dRhodamine terminator cycle
sequencing kit (Applied Biosystems, Foster City, CA) (Fig. 1).
Statistical Analysis
Frequency tables of independent variables and Ser-249 p53 mutations, as a dichotomous
variable, were evaluated for statistical significance by Pearson's 2 test
and Fisher's exact test. For the estimation of the risk of mutations among the different
groups in the study while adjustment was made for potential confounders (including age, sex,
recruitment site, and hepatitis B surface antigen [HBsAg] status, a marker of chronic
infection with HBV), a multivariable logistic regression analysis was performed to estimate odds
ratios (ORs) and 95% confidence intervals (CIs). In a separate stratified analysis, only data
from patients with HCC were evaluated by similar methods to examine factors associated with
Ser-249 p53 mutation-positive HCC compared with Ser-249 p53 mutation-negative HCC. All
analyses were performed with STATA version 5.0 (Stata Corporation, College Station, TX) and
EPI-Info version 6.0 (Centers for Disease Control and Prevention, Atlanta, GA; and World
Health Organization, Geneva, Switzerland). All P values are two-sided.
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RESULTS |
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When the analysis among only patients with HCC was stratified by mutation status, no
difference in Ser-249 p53 mutation prevalence was observed by age or HBsAg status (Table 3). Females were again overrepresented in the Ser-249 p53
mutation-positive group; however, after adjustment for age, recruitment site, and HBsAg status,
the strength of the association was of borderline statistical significance (P =
.053).
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DISCUSSION |
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Circulating plasma DNA can be retrieved and analyzed for genetic alterations present in the original tumor. In some studies (16-18,20,21), various genetic alterations, such as loss of heterozygosity or K-ras mutations, have been identified in the plasma or sera and tumor tissue from patients with cancer of the lung, head and neck, kidney, colon, and pancreas. These observations and the absence or rarity of alterations in control subjects provide strong evidence that the altered DNA, circulating in the plasma or sera of the patient, originates from the tumor. Although knowledge of the underlying mechanisms of this circulating DNA is still limited, there is some evidence that the DNA is released from the tumor as a glyconucleoprotein complex that may protect it from degradation by nucleases. It remains unclear whether release of tumor DNA into plasma is associated with tumor necrosis, apoptotic cell death, or other selective cellular processes (22-24).
Despite these uncertainties, the data that we present on Ser-249 p53 mutations are consistent with the premise that plasma or serum may be used as a source of tumor-specific DNA. In addition, the data show that this mutation is present in the plasma DNA of patients with HCC from a country with high exposure to AFB1 but not from Europe. Among 10 385 p53 mutations described in human cancers and compiled in the IARC p53 mutation database (http://www.iarc.fr/p53/) (25), a total of 197 are Ser-249 p53 mutation, 130 (66%) of which occur in patients with HCC originating from regions with a high incidence of HCC and high exposure to AFB1. Twenty-five percent of the Ser-249 p53 mutations were from lung cancers. The presence of this mutation in patients with HCC who are from Europe, the United States, Japan, and Australia is extremely low; only three mutations (one in Europe and two in Japan) were identified among 664 patients with HCC analyzed (6). These data provide strong, albeit indirect, evidence that the DNA containing the Ser-249 p53 mutation originates from the liver. In most developing countries, autopsies or biopsies for HCC are rarely performed; likewise, in this study, we did not have access to tumor tissue from the patients who donated the plasma samples. This is an important limitation of the present study. Collection of paired tumor and plasma samples for confirmation of concordant mutation status is under way in The Gambia. In neighboring Senegal, the prevalence of Ser-249 p53 mutations was reported to be approximately 67% in a series of 15 patients with HCC (26). The limited data available in The Gambia on the Ser-249 p53 mutation in HCC tumors are consistent with these findings (data not shown). Thus, we estimate that around 70% of patients with HCC who have a detectable Ser-249 p53 mutation in their tumor might also have detectable amounts of mutated DNA in their plasma.
Detection of the Ser-249 p53 mutation in circulating DNA depends on the ability of the method used to detect mutant DNA in a background of wild-type p53 DNA, which is estimated at 1 : 64 in the present study, based on serial dilution experiments. Preliminary results from the potentially more sensitive mass spectrometry analysis (27,28) of PCR products obtained with different primers also demonstrated the presence of Ser-249 p53 mutation in plasma samples from The Gambia (Friesen M: personal communication). By increasing the sensitivity of this method, DNA with a Ser-249 p53 mutation may be detected in more individuals.
It is interesting that, in this study, Ser-249 p53 mutations were detected not only in patients with HCC but also in patients with cirrhosis (15%) and a small proportion of control subjects (6%). The Ser-249 p53 mutation has been reported previously in nonmalignant liver tissue at frequencies that paralleled the levels of exposure to AFB1, suggesting that this mutation could be an early genetic event in hepatocarcinogenesis (29). Detection of the Ser-249 p53 mutation in DNA may thus be useful as a marker of neoplastic development. Alternatively, the presence of the mutation in healthy subjects may reflect chronic exposure to high levels of AFB1. The Ser-249 p53 mutation in plasma DNA could be a marker of disease (an early neoplastic effect), exposure (a biologic effect of aflatoxin exposure), or both. Consequently, detection of this mutation may perhaps identify high-risk individuals who could benefit from more intensive evaluation that could detect HCC earlier.
There is substantial evidence that the Ser-249 p53 mutation present in HCC is directly attributable to DNA damage induced by exposure to dietary AFB1 (6,30). This proposal is based on the following observations [see (7)]: 1) AFB1 is highly mutagenic and carcinogenic (3); 2) DNA lesions induced by AFB1 at guanine residues in double-stranded DNA are by no means random and are dependent on flanking nucleotide sequences (31,32); 3) guanine-to-thymine transversion is the prevalent base change in mutations induced by AFB1 (33,34); 4) the AFB1 metabolite binds specifically to the third nucleotide (AGG) in codon 249 (29,35); and 5) the Ser-249 p53 mutation is present in nontumorous tissues in patients from populations at high risk for HCC (29). One recent article (36) that questioned the association between Ser-249 p53 mutations and exposure to AFB1 was based on a highly selective literature review [see (6,7)] and data from an in vitro system that does not mimic the in vivo selective advantage of cells containing this specific mutation [see(7)]. At the same time, the Ser-249 p53 mutation has been reported in cultured human cells treated with oxygen radicals (37).
Among patients with HCC, we did not observe any differences in chronic infection with HBV
(based on HBsAg status) between those patients with HCC who were positive for the Ser-249
p53 mutation in plasma DNA and patients with HCC who were negative for this mutation (Table
3). Therefore, the highly statistically significant increased risk of HCC
associated with Ser-249 p53 mutations in plasma DNA may be, at least in The Gambia,
independent of the effects of chronic HBV infection. Geographic and ecologic studies of HCC
have been limited by the fact that regions with a high incidence of HCC frequently have a
population that is simultaneously exposed to AFB1 and HBV. Other studies have been
limited by the short period of exposure reflected by urinary or serum aflatoxin biomarkers.
Because of this complexity, there are at present limited epidemiologic data to permit an estimation
of the cancer risk attributable to AFB1 exposure that is independent of HBV infection
and the risk attributable to an interaction between these two factors (38).
A recent prospective study of Chinese HBV carriers showed a threefold increased risk for HCC
among individuals with detectable aflatoxin levels, as determined by urinary biomarker
measurements on pooled urine samples (39,40). This observed effect of
aflatoxin with HBV is similar to the independent effect but notably less than the multiplicative
interaction associated with aflatoxin biomarkers observed in the original Chinese studies by Ross
and colleagues (4,5). Likewise, our data are consistent with an
independent causal association between AFB1 exposure, HBV carrier status, and the
subsequent risk of HCC. The use of the Ser-249 p53 mutation detection in plasma or serum DNA
as a marker of aflatoxin exposure, along with serologic markers of HBV status, may provide a
better estimation of the risk attributable to these agents and a better understanding of their
interaction in cancer development. Such studies will have a direct bearing on evaluating the effect
of HBV vaccination in preventing HCC presently under way in The Gambia (41).
Despite the 4 : 1 preponderance of males among patients with HCC, females were
consistently more likely to have the Ser-249 p53 mutation. At present, to our knowledge, no data
are available to suggest that the prevalence of Ser-249 p53 mutations in HCC tumors might be
higher in females than in males. Further studies are needed to confirm this observed sex difference
(Table 3).
In addition to p53 mutations, other alterations have been reported in HCC in the genes controlling the transition from G1 to S phase of the cell cycle, such as amplification of the cyclin D1 gene (42) and inactivation of expression of the p16INK4 gene, resulting from hypermethylation of this gene (43). A recent study (18) reported a high prevalence of aberrant methylation of the p16 gene in serum DNA from patients with HCC. Somatic mutations in the ß-catenin gene (44) and other genetic changes (45) are also frequently detected in HCC tumors. The possibility of detecting these genetic or epigenetic changes in blood specimens from patients with HCC should further elucidate the natural history of HCC and eventually assist in earlier detection and treatment of this cancer.
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NOTES |
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We thank the patients and their families for their participation; the administrative and clinical staff of the Royal Victoria Hospital, Medical Research Council Hospital, and Bansang Hospital; the staff of the Medical Research Council Laboratories at Fajara and Basse, The Gambia; the Research Triangle Institute, Research Triangle Park, NC, for project management; and M. Wrisez for administrative support.
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Manuscript received July 19, 1999; revised October 28, 1999; accepted November 10, 1999.
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