REPORT

Effect of Retroviral Endostatin Gene Transfer on Subcutaneous and Intraperitoneal Growth of Murine Tumors

Andrew L. Feldman, H. Richard Alexander, Stephen M. Hewitt, Dominique Lorang, Christina E. Thiruvathukal, Ewa M. Turner, Steven K. Libutti

Affiliations of authors: A. L. Feldman, H. R. Alexander, D. Lorang, C. E. Thiruvathukal, E. M. Turner, S. K. Libutti (Surgical Metabolism Section, Surgery Branch, Center for Cancer Research), S. M. Hewitt (Laboratory of Pathology, Center for Cancer Research), National Cancer Institute, Bethesda, MD.

Correspondence to: Steven K. Libutti, M.D., National Institutes of Health, Bldg. 10, Rm. 3C428, Bethesda, MD 20892 (e-mail: Steven_Libutti{at}nih. gov).


    ABSTRACT
 Top
 Abstract
 Introduction
 Notes
 Materials and Methods
 Results
 Discussion
 References
 
Background: Inhibiting tumor angiogenesis is a promising new strategy for treating cancer. Difficulties with the stability, manufacture, and long-term administration of recombinant antiangiogenic proteins have prompted investigators to use gene therapy to generate these proteins in vivo. We investigated whether transfer of the gene encoding the angiogenesis inhibitor endostatin into the murine liver cell line NMuLi could inhibit tumor growth in vivo. Methods: NMuLi cells were transduced with retroviral vectors containing the murine endostatin gene. The presence and function of endostatin in transduced cell supernatants were confirmed by competitive enzyme immunoassay and endothelial cell proliferation assays. Nude mice were given a subcutaneous or intraperitoneal injection with NMuLi cells, control transduced cells (NEF-null), or endostatin-transduced clones (NEF-Endo1 to 4) and were monitored for tumor growth. All statistical tests were two-sided. Results: Supernatants from the clone secreting the lowest amount of endostatin (NEF-Endo4, 28 ng/mL) inhibited endothelial cell proliferation by 6% (95% confidence interval [CI] = 0% to 12%), and those from the clone secreting the highest amount (NEF-Endo1, 223 ng/mL) inhibited endothelial cell proliferation by 20% (95% CI = 13% to 27%). Increased levels of endostatin were detected in tumor lysates, but not serum, of mice given a subcutaneous injection of NEF-Endo1 cells. After 63 days, mice given a subcutaneous injection of parental NMuLi or NEF-null cells had tumor volumes of 2400 mm3 (95% CI = 1478 mm3 to 3300 mm3) and 2700 mm3 (95% CI = 2241 mm3 to 3144 mm3), respectively, compared with mean tumor volumes of less than 30 mm3 in mice given an injection of NEF-Endo clones, a statistically significant difference (P<.001). After 123 days, all 16 mice given an intraperitoneal injection of parental NMuLi or NEF-null cells had died, compared with only three (9%) of 32 mice given an injection of NEF-Endo clones. Conclusions: Retroviral endostatin gene transfer leads to secretion of functional endostatin that is sufficiently active to inhibit tumor growth. Further studies of retroviral endostatin gene transfer for the treatment of cancer are warranted.



    INTRODUCTION
 Top
 Abstract
 Introduction
 Notes
 Materials and Methods
 Results
 Discussion
 References
 
Because tumors require angiogenesis for sustained growth (1), inhibiting tumor angiogenesis is a promising new strategy for treating cancer patients. However, difficulties in the stability, manufacture, and long-term administration of recombinant forms of endogenous antiangiogenic proteins have led investigators to develop gene therapy approaches to the antiangiogenic treatment of cancer (2). Endostatin, a 20-kd C-terminal fragment of collagen XVIII (3), is a potent antiangiogenic agent currently being evaluated in clinical trials (4).1 We tested whether retroviral transfer of the endostatin gene could generate sufficient functional endostatin in vivo to inhibit tumor growth.


    MATERIALS AND METHODS
 Top
 Abstract
 Introduction
 Notes
 Materials and Methods
 Results
 Discussion
 References
 
Generation of Pseudotyped Retroviral Particles

The retroviral vector pCLNCX (5) and the vector pMD.G containing the G protein gene from vesicular stomatitis virus (VSV), as well as the cell line 293GP stably transfected with the retroviral gag and pol elements, were obtained from P. Robbins, National Cancer Institute (NCI), Bethesda, MD. The human elongation factor (EF) 1{alpha} promoter was isolated from the plasmid pAd.EF1 (Z. Guo, NCI) by BamHI/HindIII digestion (New England Biolabs Inc., Beverly, MA) and ligated in place of the downstream cytomegalovirus (CMV) promoter in BamHI/HindIII-digested pCLNCX to generate the vector pEF-null (Fig. 1Go, a). Generation of the construct ss-mEndo, consisting of the 18-amino acid E3/19K signal sequence followed by the murine endostatin gene cloned from murine liver, has been described previously (6). The ss-mEndo construct was cloned into HindIII/ClaI-digested pEF-null to generate the vector pEF-Endo or into HindIII/ClaI-digested pCLNCX to generate the vector pCMV-Endo.



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Fig. 1. Production and in vitro characteristics of retrovirally transduced NMuLi cells. Panel a: schematic representations of the retroviral vectors used in this study, with the supernatant endostatin concentrations, determined by competitive enzyme immunoassay (EIA), resulting from transduction of NMuLi cells and selection of G418-resistant cell populations (right). The murine endostatin gene was cloned into pCLNCX (5); however, NMuLi transduction resulted in minimal increases of supernatant endostatin levels above baseline. Substitution of the downstream cytomegalovirus (CMV) enhancer promoter with the human elongation factor (EF) 1{alpha} promoter yielded substantial elevation of the supernatant endostatin concentration. Panel b: western blot demonstrating secreted endostatin protein in transduced cell culture supernatants after 24 hours. Arrow at left denotes 20-kd molecular mass. Lanes 1, 2, and 3—recombinant murine endostatin at concentrations of 500, 125, and 31 ng/mL, respectively; lane 4—water; lane 5—NEF-Endo1 supernatant (endostatin-competitive EIA concentration, 223 ng/mL); lane 6—NEF-Endo2 supernatant (endostatin-competitive EIA concentration, 163 ng/mL); lane 7—NEF-Endo3 supernatant (endostatin-competitive EIA concentration, 42 ng/mL); lane 8—NEF-Endo4 supernatant (endostatin-competitive EIA concentration, 28 ng/mL); lane 9—NEF-null supernatant (endostatin-competitive EIA concentration, 20 ng/mL); and lane 10—parental NMuLi supernatant (endostatin-competitive EIA concentration, 4 ng/mL). Panel c: The ability of culture supernatants from transduced cells to inhibit bovine capillary endothelial cell proliferation was measured after 72 hours by the WST-1 colorimetric assay. Inhibition is compared with unconditioned medium containing 1 ng/mL of basic fibroblast growth factor. Inhibition by NEF-Endo1 supernatant was statistically significantly higher than inhibition by NEF-null supernatant (P = .037), and there was a statistically significant trend of increasing inhibition with increasing endostatin concentration (P=.01). Panel d: In vitro proliferation of transduced cells was measured daily by the WST-1 colorimetric assay. Results are expressed as the change in optical density ({Delta}OD) at 450 nm after incubation with the tetrazolium salt WST-1. Each data point represents the mean value of eight identical wells. In vitro growth characteristics were similar among the transduced cell lines. CI = confidence interval.

 
293GP cells were maintained in complete medium consisting of Dulbecco's modified Eagle medium (DMEM) with 10% fetal calf serum (FCS), 100 U/mL of penicillin, 100 µg/mL of streptomycin, 50 µg/mL of gentamicin, 0.5 µg/mL of Fungizone, and 4 mM glutamine (Biofluids, Rockville, MD). Cells were plated in 10-cm tissue culture dishes at a density of 3 x 106 cells/dish and allowed to adhere overnight in a 5% CO2 incubator at 37 °C. Cells then were rinsed with phosphate-buffered saline (PBS), cotransfected with 6 µg of retroviral plasmid and 6 µg of pMD.G (for VSV envelope protein expression) by use of 60 µL of Lipofectamine (Life Technologies, Inc. [GIBCO BRL], Rockville, MD) in 6 mL of serum-free DMEM, and incubated at 37 °C for 3 hours. After the addition of 12 mL of complete medium, cells were further incubated for 24 hours before the medium was replaced with 10 mL of fresh complete medium. After 24 hours, the supernatant containing the retroviral particles was collected, passed through a 0.45-µm (pore size) filter, and stored at -70 °C until ready for use.

Retroviral Transduction of NMuLi Cells

The cell line NMuLi, an epithelioid nonparenchymal cell line derived from the NAMRU mouse liver (7), was obtained from the American Type Culture Collection (Manassas, VA) and passaged in complete medium. We confirmed its ability to form malignant tumors in nude mice (8). For the retroviral transduction, cells were plated in six-well tissue culture plates at a density of 100 000 cells/well and allowed to adhere overnight. The medium then was replaced with 1 mL of fresh complete medium, 1 mL of retroviral supernatant, and 8 µg/mL of hexadimethrine bromide (Sigma Chemical Co., St. Louis, MO) to assist the uptake of viral particles. After incubation at 37 °C for 4 hours, the medium was replaced with 2 mL of fresh complete medium. The transduction was repeated the following day. Because the retroviral vector contains a selectable marker, the neomycin resistance gene, G418 (400 µg/mL; Life Technologies, Inc.), was added to the medium 24 hours after the second transduction. The surviving G418-resistant cells were amplified. To obtain individual clones, the endostatin-transduced cells (NEF-Endo) were plated in limiting dilution in complete medium containing G418 (400 µg/mL). The resultant clones were isolated by use of 8-mm diameter cloning cylinders (Specialty Media, Phillipsburg, NJ) and amplified.

To confirm endostatin expression, parental NMuLi cells, pEF-null-transduced cells (NEF-null), or NEF-Endo clones were plated in six-well plates at a density of 106 cells/well and allowed to adhere overnight. The medium was replaced with 1 mL/well of modified complete medium containing 5% FCS and incubated at 37 °C for 24 hours. Cell supernatants then were collected and passed through a 0.45-µm (pore size) filter. Endostatin concentrations in the supernatants were determined by competitive enzyme immunoassay (EIA) (Cytimmune Sciences, College Park, MD) according to the instructions of the manufacturer. Four endostatin-transduced clones with varying supernatant endostatin concentrations were selected for further study and designated NEF-Endo1 to 4, in decreasing order of endostatin production. The molecular weight of endostatin was determined from the culture supernatants by western blotting (NuPAGE; Novex, San Diego, CA) by use of 570 ng/mL of rabbit antimurine endostatin polyclonal immunogloblin G antibody (from Cytimmune Sciences). The EIA murine endostatin standard was used as a positive control.

Functional Assay of Retrovirally Derived Endostatin

To assess the functional activity of the retrovirally derived endostatin, we tested the culture supernatants in endothelial cell proliferation assays as described previously (6), with slight modifications. Briefly, bovine adrenal capillary endothelial cells (EJG; American Type Culture Collection) were plated in 200 µL of complete medium in collagen I-coated 96-well plates (Biocoat; Becton Dickinson Labware, Bedford, MA) at a density of 1000 cells/well and were incubated overnight at 37 °C. The medium then was aspirated and replaced with 100 µL of cell supernatant or modified complete medium containing 5% FCS. Basic fibroblast growth factor (R&D Systems, Inc., Minneapolis, MN) at a final concentration of 1 ng/mL was added to all wells as a stimulus of proliferation. After incubation at 37 °C for 72 hours, proliferation was analyzed by the WST-1 assay (Roche, Indianapolis, IN) according to the manufacturer's instructions. The inhibition of proliferation by each sample was calculated according to the formula: inhibition (%) = (mean {Delta}ODmedia{Delta}ODsample/mean {Delta}ODmedia) x 100, where {Delta}OD represents the change in optical density at 450 nm (Multiskan MCC/340 plate reader; Titertek, Huntsville, AL) from before the addition of the WST-1 reagent to the completion of the 4 hours' incubation at 37 °C. Each sample was measured in six individual wells. The experiment was repeated to confirm results.

In Vitro Growth of Parental and Transduced NMuLi Cells

To place in perspective any differences noted in the in vivo growth of parental and transduced cell lines, we compared in vitro growth rates. Five identical 96-well tissue culture plates were prepared. On each plate, NMuLi, NEF-null, and NEF-Endo1 to 4 cells were each plated in eight identical wells at a density of 1000 cells/well in 100 µL of complete medium. WST-1 proliferation assays were performed daily on days 0–4, as described above. Cells were allowed to adhere for 3 hours before the day-0 assay was performed. Proliferation was expressed as the {Delta}OD as defined above.

Tumor Formation by Retrovirally Transduced Cells

Animal experiments were conducted according to protocols approved by the National Institutes of Health Animal Care and Use Committee (Bethesda, MD). Eight-week-old female nude mice (Charles River Laboratories, Wilmington, DE) were given an injection of 5 x 105 parental NMuLi cells, NEF-null cells, or NEF-Endo1 to 4 cells. Subcutaneous injections were administered in the right flank in 100 µL of PBS. Tumors were measured in two dimensions by use of calipers at regular intervals, and tumor volumes were calculated according to the following formula: volume = width2 x length x 0.52, where 0.52 is a constant to calculate the volume of an ellipsoid. Intraperitoneal injections were administered in 2 mL of PBS. All animals were followed for survival. Each group consisted of eight mice.

Measurement of Endostatin Levels In Vivo

Mice were given an inoculation of NEF-null or NEF-Endo1 cells subcutaneously or intraperitoneally as described above. Serum samples obtained 45 days (subcutaneous model) or 21 days (intraperitoneal model) after tumor cell inoculation were analyzed for endostatin concentration by EIA. Serum samples also were obtained from non-tumor-bearing animals. Each group consisted of six mice.

To assess in vivo local endostatin expression, we harvested NEF-null or NEF-Endo1 tumors immediately after the mice were killed 38 days after subcutaneous injection as described above. Tumors were snap-frozen in liquid nitrogen and stored at -80 °C until ready for analysis. Tumors then were homogenized at room temperature in a protease inhibitor cocktail (Complete Mini; Roche) dissolved in 10 mM HEPES and 1 mM EDTA by use of a bead homogenizer (FastPrep; Savant Instruments, Holbrook, NY). Homogenates were centrifuged at 7500g for 5 minutes at 4 °C, and supernatants were analyzed for endostatin by EIA. Endostatin concentrations were normalized to total protein concentrations determined by use of a BCA protein assay kit (Pierce Chemical Co., Rockford, IL) and a linear bovine serum albumin standard curve, according to the manufacturer's instructions.

Immunohistochemistry and Histopathologic Evaluation

NEF-null or NEF-Endo1 tumors were harvested immediately after the mice were killed 38 days after subcutaneous injection, as described above. Tumors were snap-frozen in liquid nitrogen and stored at -80 °C. Frozen sections (7 µm) were cut on a cryostat and stained with hematoxylin–eosin (H&E) or antibodies specific for CD31 (PharMingen, San Diego, CA), proliferating cell nuclear antigen (PCNA) (Zymed Laboratories, San Francisco, CA), or caspase-3 (PharMingen). For immunostaining, sections were fixed in acetone for 10 minutes (CD31) or in 4% formalin for 1 hour (PCNA and caspase-3). After endogenous peroxidase activity was blocked by use of 3% hydrogen peroxide in methanol for 10 minutes, sections were incubated for 1 hour in a blocking solution containing 10% normal goat serum. Sections were incubated with primary antibody at 4 °C overnight (CD31 at 1 : 50 dilution or caspase-3 at a concentration of 1 µg/mL) or at room temperature for 1 hour (PCNA at 1 : 50 dilution). Slides were then washed three times in PBS, incubated in biotinylated species-specific appropriate secondary antibody for 1 hour, and exposed to avidin–biotin–peroxidase complex (Vector Laboratories, Inc., Burlingame, CA). Sections were reacted with 0.06% 3,3'-diaminobenzidine (Sigma Chemical Co.) and counterstained with hematoxylin.

H&E and immunostained sections were analyzed by a pathologist (S. M. Hewitt), who was blinded to the identity of the groups. Only good-quality sections with uniform, well-demarcated staining and low background were analyzed. Microvascular density of each tumor was assessed by use of a scoring system (Table 1Go) based on the mean number of CD31-positive cells per high-power field (hpf, x600). Proliferative and apoptotic cells were analyzed by use of a similar scoring system (Table 1Go) based on the number of cells per hpf staining positively for PCNA and caspase-3, respectively.


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Table 1. Histopathologic findings in transduced NMuLi tumors*
 
Statistical Analysis

Data are presented as means with 95% confidence intervals (CIs). Comparisons between groups were made by use of the Mann–Whitney U test or the Kruskal–Wallis test, where appropriate. The endostatin dose dependence of endothelial cell inhibition was analyzed by use of the Jonckheere–Terpstra trend test. Two-tailed P values <.05 were considered to be statistically significant.


    RESULTS
 Top
 Abstract
 Introduction
 Notes
 Materials and Methods
 Results
 Discussion
 References
 
In Vitro Production of Endostatin

Initial transduction of NMuLi cells with pCMV-Endo did not yield supernatant endostatin concentrations above baseline. We, therefore, replaced the downstream CMV promoter in pCLNCX with the EF1{alpha} promoter (Fig. 1Go, a) based on favorable results reported with the use of the EF1{alpha} promoter in other viral gene delivery systems (9,10). Endostatin levels in supernatants from NEF-Endo1, 2, 3, and 4, NEF-null, and NMuLi cells were 223, 163, 42, 28, 20, and 4 ng/mL per 106 cells, respectively. The NEF-Endo clone supernatants produced specific 20-kd bands on western blot proportional in intensity to the endostatin concentration as measured by EIA (Fig. 1Go, b).

In Vitro Function of Endostatin

To determine if the endostatin produced by the transduced clones was biologically active, we tested the supernatants in an endothelial cell proliferation assay. Compared with unconditioned medium, supernatants from the transduced clones NEF-Endo 1, 2, 3, and 4, and NEF-null inhibited endothelial cell proliferation by 20% (95% CI = 13% to 27%), 11% (95% CI = 3% to 19%), 9% (95% CI = –1% to 19%), 6% (95% CI = 0% to 12%), and 4% (95% CI = –8% to 17%), respectively (Fig. 1Go, c). The difference in inhibition between supernatants from NEF-Endo1 and NEF-null was statistically significant (P = .037). The dose-dependent inhibition of endothelial cell proliferation by endostatin also was statistically significant (P = .01).

In Vitro Growth of Parental and Transduced NMuLi Cells

We next compared the in vitro growth of the transduced cells with that of the parental NMuLi cells. All four NEF-Endo clones demonstrated similar patterns of in vitro growth to each other and to NEF-null cells (Fig. 1Go, d). Parental NMuLi cells grew more slowly in vitro than their transduced, G418-selected counterparts.

Subcutaneous Growth of Parental and Transduced NMuLi Cells

Mice given a subcutaneous injection of NMuLi or NEF-null cells developed rapidly growing tumors and were killed 63 days after injection when the tumor volumes were 2400 mm3 (95% CI = 1478 mm3 to 3300 mm3) and 2700 mm3 (95% CI = 2241 mm3 to 3144 mm3), respectively (Fig. 2Go, a). The mean tumor volumes were less than 30 mm3 in all four groups given an injection of NEF-Endo clones (P.<001 versus null-transduced tumors). Although the largest endostatin-transduced tumors were observed in the mice that were given an injection of the NEF-Endo4 clone, which produced the lowest amount of endostatin in vitro (NEF-Endo4; Fig. 2Go, a, inset), the difference between the size of the endostatin-transduced tumors was not statistically significant (P = .52 at day 63). After 122 days of follow-up, mice given an injection of NEF-Endo clones 1, 3, and 4 had tumor volumes of 70 mm3 (95% CI = 0 mm3 to 202 mm3), 300 mm3 (95% CI = 123 mm3 to 419 mm3), and 90 mm3 (95% CI = 0 mm3 to 279 mm3), respectively. Mice given an injection of the NEF-Endo clone 2 had nonpalpable or barely palpable lesions.



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Fig. 2. In vivo characteristics of retrovirally transduced NMuLi cells. Panel a: assessment of tumor volume in mice given a subcutaneous injection of 5 x 105 parental NMuLi cells ({square} ), NEF-null cells ({blacksquare}), or NEF-Endo clones 1 (•), 2 ({circ}), 3 ({blacktriangleleft}), and 4 ({triangleleft}). Each point represents the mean volume of eight mice. Inset shows expanded y-axis to demonstrate tumor volumes of NEF-Endo clones. Control (NMuLi and NEF-null) mice were killed on day 63, at which time the mean tumor volumes among NEF-Endo groups were less than 30 mm3. Panel b: mouse survival after intraperitoneal inoculation of parental and transduced NMuLi cells. Each group contained eight mice. Median survival times were 58 days for mice receiving NMuLi cells and 56 days for mice receiving NEF-null cells. All control animals died by day 123, at which time only three (9%) of the 32 animals receiving NEF-Endo clones had died. The numbers of mice at risk in each group after 60 days were as follows: NMuLi = 2 (95% confidence interval [CI] = 0.2 to 3.8); NEF-null = 3 (95% CI = 0.6 to 4.7); NEF-Endo1 = 8 (95% CI = 5.0 to 8.0); NEF-Endo2 = 8 (95% CI = 5.0 to 8.0); NEF-Endo3 = 8 (95% CI = 5.0 to 8.0); and NEF-Endo4 = 8 (95% CI = 5.0 to 8.0). The numbers of mice at risk in each group after 120 days were as follows: NMuLi = 0; NEF-null = 1 (95% CI = 0.0 to 4.2); NEF-Endo1 = 7 (95% CI = 4.2 to 7.8); NEF-Endo2 = 6 (95% CI = 3.3 to 7.4); NEF-Endo3 = 8 (95% CI = 5.0 to 8.0); and NEF-Endo4 = 8 (95% CI = 5.0 to 8.0).

 
Intraperitoneal Growth of Parental and Transduced NMuLi Cells

Mice given an intraperitoneal injection of parental NMuLi or NEF-null cells had median survival times of 58 days (95% CI = 49 days to 61 days) and 56 days (95% CI = 46 days to 92 days), respectively, and all mice were dead by day 123 (Fig. 2Go, b). At this time, only three (9%) of the 32 mice receiving intraperitoneal NEF-Endo clones had died. Autopsies of mice that died revealed massive peritoneal tumor deposits and ascites. Surviving mice were killed and had an autopsy, revealing only occasional, small peritoneal deposits.

In Vivo Endostatin Levels

Serum endostatin levels were similar in mice bearing subcutaneous NEF-Endo1 tumors (mean, 33.8 ng/mL; 95% CI = 18.5 ng/mL to 49.1 ng/mL) and NEF-null tumors (mean, 32.6 ng/mL; 95% CI = 15.8 ng/ml to 49.4 ng/mL), respectively (P = .87). Serum endostatin levels in mice bearing intraperitoneal NEF-Endo1 tumors (mean, 40.0 ng/mL; 95% CI = 7.8 ng/mL to 72.2 ng/mL) were slightly higher than those in mice bearing intraperitoneal NEF-null tumors (mean, 22.2 ng/mL; 95% CI = 18.3 ng/mL to 26.1 ng/mL), but this difference was not statistically significant (P = .42). Serum endostatin levels in non-tumor-bearing mice were 27.1 ng/mL (95% CI = 15.3 ng/mL to 38.9 ng/mL), similar to values we reported previously (11) and not statistically significantly different from any of the other groups tested.

To confirm endostatin production in the NEF-Endo tumors, we measured local endostatin levels in tumor lysates 38 days after subcutaneous injection. Tumor lysates from endostatin- and null-transduced tumor had 38.7 ng (95% CI = 20.7 ng to 56.6 ng) and 11.2 ng (95% CI = 8.8 ng to 13.7 ng) endostatin/mg total protein, respectively (P = .006).

Immunohistologic Characteristics of Transduced Tumors

Evaluation of H&E-stained tumor sections revealed small tumor nodules (mean diameter, 1.7 mm) derived from NEF-Endo1 and NEF-Endo2 cells (Table 1Go; Fig. 3Go, a); tumors derived from NEF-null cells were larger (mean diameter, 4.9 mm) and had zones of central necrosis (Fig. 3Go, b). Mean microvessel density scores for endostatin- and null-transduced tumors were 3 and 4, respectively (Table 1Go; Fig. 3Go, c–e). Endostatin-transduced tumors did not have discernible vessels on H&E stains (Fig. 3Go, a) but did contain CD31-positive endothelial cells as individual capillaries (Fig. 3Go, c). By contrast, the null-transduced tumors contained easily identifiable vessels on H&E stains, away from zones of necrosis (Fig. 3Go, b). The CD31 staining highlighted larger vessels as well as individual endothelial cells (Fig. 3Go, d).



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Fig. 3. Histopathologic findings in transduced NMuLi tumors. Panel a: representative section from an endostatin-transduced tumor (hematoxylin–eosin [H&E], original magnification x200; scale bar = 50 µm). Panel b: representative section from a null-transduced tumor (H&E, original magnification x200; scale bar = 50 µm). Panel c: microvessel staining from a representative section of an NEF-Endo1 tumor (anti-CD31 immunostaining, original magnification x400; scale bar = 25 µm). Panel d: microvessel staining from a representative section of an NEF-null tumor (anti-CD31 immunostaining, original magnification x400; scale bar = 25 µm). Panel e: Mean tumor diameters and scores for CD31, caspase-3, and proliferating cell nuclear antigen (PCNA) immunostaining in endostatin- and null-transduced tumors.

 
Finally, we determined the apoptotic and proliferative scores for the endostatin- and null-transduced tumors. The null-transduced tumors had higher apoptotic and proliferative scores than the endostatin-transduced tumors, with mean apoptosis scores for endostatin- and null-transduced tumors of 1 and 2.3, respectively, and mean proliferative scores of 3 and 4, respectively (Table 1Go; Fig. 3Go, e).


    DISCUSSION
 Top
 Abstract
 Introduction
 Notes
 Materials and Methods
 Results
 Discussion
 References
 
The demonstration that tumors require neoangiogenesis for sustained growth (1) has prompted intense investigation into the inhibition of tumor angiogenesis as a strategy for treating cancer patients. More than 40 endogenous inhibitors of angiogenesis have been described previously (2). Of interest, some of these inhibitors are tumor derived, including endostatin, a 20-kd C-terminal fragment of collagen XVIII originally isolated from a murine hemangioendothelioma cell line (3). There are, however, a number of potential limitations to treating cancer with antiangiogenic agents, such as endostatin, in their recombinant forms. First, some of these recombinant biologics, including endostatin, are relatively unstable in vitro and require high doses for antitumor efficacy (3). Second, many of these agents are not cytotoxic to tumor cells themselves and may need to be administered chronically (12). Finally, systems that allow continuous delivery of antiangiogenic agents appear to be therapeutically preferable to the peak/trough kinetics associated with bolus infusion of recombinant proteins (13,14). These limitations have prompted investigators to study gene therapy approaches to deliver antiangiogenic proteins for the treatment of cancer (2).

Previously, we and others (6,15,16) have shown that adenoviral transfer of the endostatin gene to mice leads to high circulating endostatin levels that inhibit tumor growth. Several groups (1719) also have demonstrated that nonviral endostatin gene transfer can cause an antitumor effect in mice. Both of these approaches, however, are associated with limitations in their ability to be translated for the treatment of human patients: The use of adenoviral vectors is limited because of immunogenicity and toxicity, and nonviral vectors generally are associated with low levels of transgene expression. Because retroviruses have been used safely in human gene therapy trials (20,21), we investigated whether retroviral transfer of the endostatin gene to tumor cells could result in sufficient functional protein to inhibit tumor growth in vivo.

Because endostatin occurs naturally as a cleavage product of collagen XVIII, the coding sequence for endostatin lacks a secretion signal. We previously generated a construct in which the endostatin coding sequence is preceded by the adenoviral protein E3/19K signal sequence (6,22). We cloned this construct into the multiple cloning site of pCLNCX, a Moloney murine leukemia virus-derived retroviral expression plasmid (5), and generated pseudotyped retroviral particles containing the VSV glycoprotein G (23). NMuLi cells transduced with this construct were resistant to G418, but they did not generate high levels of endostatin in their cell supernatants. We hypothesized that this was a promoter-related phenomenon and replaced the downstream CMV promoter in the pCLNCX plasmid with the constitutively active human EF-1{alpha} promoter. Cells transduced with this modified construct secreted high endostatin levels, as measured in a competitive EIA.

To verify that the secreted protein measured in the EIA was, in fact, endostatin, we performed western blotting of aliquots from supernatants of the transduced cells. Supernatants from NEF-Endo clones produced bands of equal mobility to that of recombinant murine endostatin and of intensities proportional to the EIA-measured endostatin concentrations. When these supernatants were placed on bovine adrenal capillary endothelial cells, they inhibited endothelial cell proliferation in a dose-dependent fashion, confirming the activity of the secreted endostatin protein. The secreted endostatin was relatively endothelial cell specific, as has been reported previously (3), because the growth rates of the NEF-null cells and the NEF-Endo clones were similar, including NEF-Endo1 cells that secreted the highest level of endostatin.

By contrast with the in vitro growth, transduction of NMuLi cells with the endostatin gene profoundly inhibited their subcutaneous growth in mice. Consistent with the antiangiogenic function of endostatin, NEF-Endo cells were capable of forming tumors in vivo, although these tumors were very small and displayed fewer microvessels than their null-transduced counterparts. The NEF-Endo4 clone, which produced the least endostatin in vitro, initially produced larger tumors than the other NEF-Endo clones tested. However, the inhibition of in vivo tumor growth of all clones was not directly proportional to in vitro endostatin expression. In fact, the growth curves of the four NEF-Endo clones began to diverge after approximately 100 days, although tumors in all groups remained much smaller than tumors in the NEF-null mice, which exceeded 2 cm3. The eventual divergence of the growth curves among the NEF-Endo clones growth may reflect clonal variations unrelated to endostatin. Alternatively, local endostatin production at late time points in vivo may not correspond with in vitro expression by various clones.

Of interest, endostatin transduction of NMuLi cells also inhibited their ability to form aggressive lesions in the peritoneal cavity. Like tumors in other sites, the pathogenesis of tumors of the peritoneal cavity has been shown to be associated with angiogenesis (1,24,25). In addition, several inhibitors of angiogenesis have been reported to inhibit peritoneal tumor growth in rodent models, including protamine (26), TNP-470 (2729), and agents that inhibit the action of vascular endothelial growth factor (3032). Our data suggest that endostatin also can inhibit peritoneal tumor growth. Similar to the results seen with our subcutaneous model, the tumor lesions present in mice receiving intraperitoneal NEF-Endo cells were generally small. In mice with subcutaneous or intraperitoneal tumors, circulating endostatin levels were not statistically significantly increased in mice harboring endostatin-transduced tumors. Because of the minimal amount of tumor tissue in these animals and the baseline mean serum endostatin concentration of 27 ng/mL, small amounts of endostatin released into the circulation may not have been detectable by the assay employed. The assay was, however, sensitive enough to detect increased concentrations of endostatin in lysates of endostatin-transduced tumors, confirming that endostatin was being produced locally in these animals.

Our results indicate that retroviral transfer of the endostatin gene to eukaryotic cells does not inhibit their growth in vitro but generates a secreted, functional endostatin protein that inhibits endothelial cells in vitro and tumor formation at multiple sites in vivo. In addition, these data support previous evidence (2632) that inhibiting angiogenesis is a promising strategy for treating tumors of the peritoneal cavity. However, one limitation of this study is that ex vivo transduction of tumor cells is not a strategy applicable to clinical therapeutic use. Recently, retroviruses secreting another antiangiogenic molecule, interleukin 12 (IL-12), have been used in preclinical models of disseminated peritoneal cancer. Sanches et al. (33) transduced fibroblasts with the IL-12 gene ex vivo, then introduced them into mice with peritoneal ovarian carcinomatosis. Lechanteur et al. (34) used an intraperitoneal injection of retroviral packaging cells carrying the IL-12 gene as part of a combined approach to treating peritoneal colon carcinomatosis in rats. In each case, at least part of the tumor response was because of immune (i.e., nonantiangiogenic) mechanisms, and the immune effects of IL-12 have been associated with considerable toxicity in clinical and preclinical models. By contrast, endostatin appears to impart its antitumor effect solely by inhibiting angiogenesis, with minimal toxicity(3,35). Therefore, further studies using retroviral endostatin gene transfer for the treatment of cancer, including disseminated tumors of the peritoneal cavity, are warranted.


    NOTES
 
1 An overview of angiogenesis inhibitors in clinical trials is available from URL: http://cancertrials.nci.nih.gov/news/angio/table.html (accessed April 26, 2001). Back

We thank S. Steinberg, National Cancer Institute (Bethesda, MD), for his help with the statistical analysis.


    REFERENCES
 Top
 Abstract
 Introduction
 Notes
 Materials and Methods
 Results
 Discussion
 References
 

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Manuscript received December 15, 2000; revised May 1, 2001; revised May 9, 2001;

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