BRIEF REPORT |
Evaluation of a Soybean Lectinbased Method for the Enrichment of Erythroblasts
Laboratory for Prenatal Medicine, University Women's Hospital/Department of Research, University of Basel, Basel, Switzerland (TB,SM,VK,WH,SH); NeTech, Inc., Kanagawa, Japan (HY,KK); and University Women's Hospital, University of Basel, Basel, Switzerland (OL,WH)
Correspondence to: Dr. Sinuhe Hahn, Laboratory for Prenatal Medicine, University Women's Hospital/Department of Research, Spitalstr. 21, CH 4031 Basel, Switzerland. E-mail: shahn{at}uhbs.ch
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Summary |
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(J Histochem Cytochem 53:329330, 2005)
Key Words: nucleated fetal cells enrichment CD-71 lectin
ENRICHMENT OF FETAL CELLS from the maternal blood is an alternative way to obtain fetal material for prenatal diagnosis without the considerable risks entailed in invasive procedures such as amniocentesis and chorionic villus sampling (Hahn and Holzgreve 2002). In this regard, considerable attention has been focused on fetal erythroblasts because they are abundant in the fetal circulation and rare in normal adult blood. Furthermore, because they have a short life span, they are unlikely to have persisted from a previous pregnancy, as is the case for other fetal cells, such as lymphocytes. In addition, they express molecules such as the CD-71 transferrin receptor at a very high density, which aid in their enrichment, and fetal hemoglobin molecules (
- and
-globin), which aid in their identification.
To date, the most successful approaches to the enrichment of fetal erythroblasts from maternal blood have been fluorescence-activated cell sorting and magnetic cell sorting (MACS). Consequently, the efficacy of these two methods in the enrichment of fetal erythroblasts and the detection of fetal aneuploidies was examined in a large-scale study funded by the National Institutes of Health, the so-called NIFTY study (Bianchi et al. 2002). This study indicated that neither method is suitable for potential clinical applications because both methods resulted in a poor yield of fetal erythroblasts (Bianchi et al. 2002
).
A recent study has demonstrated a new enrichment technique that uses a galactose-specific lectin for the isolation of fetal erythroblasts from maternal blood (Kitagawa et al. 2002). This method is based on the observation that erythroid precursor cells express a galactose moiety that is associated with the development and maturation of these cells. By exploiting this feature, it has been shown that the binding of this galactose moiety to a lectin, in this case soybean agglutinin (SBA), can be used in the selective enrichment of erythroblasts from maternal blood samples (Kitagawa et al. 2002
).
To evaluate this method, we performed a comparative study on 11 maternal blood samples collected at 11 to 40 weeks of pregnancy. One fraction of the blood sample was subjected to enrichment by our standard CD-71 MACS protocol (Troeger et al. 1999), and the other half was enriched by the lectin protocol (Kitagawa et al. 2002
). All samples were processed within 6 hr after blood sampling.
The lectin enrichment method was performed as described previously, except that a concentration of 100 µg/ml SBA (Vector; Geneva, Switzerland) was used (Kitagawa et al. 2002). The MACS/CD-71 enrichment was performed according to our established protocol (Troeger et al. 1999
). In both instances, the enriched cells were stained with MayGruenwald (Merck; Darmstadt, Germany)Giemsa (Sigma; Buchs, Switzerland) and the number of recovered erythroblasts was scored. For the MACS/CD-71 enrichment procedure, two slides were evaluated, whereas in the SBA-lectin method, one chamber was scored. This was used to estimate the number of recovered erythroblasts per milliliter of maternal blood.
Our results (Figure 1) indicate significant differences (p=0.00) between the two enrichment methods, with almost eightfold more erythroblasts being recovered per milliliter of maternal blood by the SBA-lectin method (median 16; range 259) compared with our standard MACS approach (median 2; range 06).
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Another apparent advantage of the SBA-lectin method is that the enriched cells appeared to have a better morphology, facilitating easier recognition and analysis, than cells treated by the MACS protocol. This facet will have to be explored in a larger study.
A major drawback of all current enrichment methods, and especially that of the SBA-lectin method, is that the final enriched cell population is contaminated by a vast number of maternal cells. Therefore, although the recovery by the SBA-lectin method is greater than that achieved by the MACS method, the purity is significantly lower. This implies that a considerable effort must still be made to locate potential fetal cells after the lectin enrichment procedure, which renders the analysis tedious and time-consuming. It will therefore be necessary to employ systems that permit the automated recognition and recovery of potential fetal cells to obtain the optimal efficiency required for clinical applications. For this reason, studies are ongoing to explore the use of systems that facilitate automated recognition of rare events and their subsequent contact-free isolation by laser pressure catapulting (Hahn et al. 2002).
In summary, our study indicates that the lectin-based method is more efficacious than a MACS approach for the enrichment of erythroblasts from maternal blood and that this method may be a promising alternative for future investigations concerned with noninvasive prenatal diagnosis.
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Footnotes |
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Received for publication May 18, 2004; accepted June 7, 2004
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Literature Cited |
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