Journal of Histochemistry and Cytochemistry, Vol. 47, 1649b-1649, December 1999, Copyright © 1999, The Histochemical Society, Inc.


PROCEEDINGS

30 Galactose oxidase-Schiff's reagent reactivity and expression of immunohistochemically detected p53 are correlated during human colorectal carcinogenesis.

J. H. Cartera, J. A. Deddensa, R. E. Richmonda, L. O. Whiteleya, J. L. Pullmana, B. M. Colligana, and H. W. Cartera
a Wood Hudson Cancer Research Laboratory, Newport, KY 41071, University of Cincinnati, Cincinnati, OH 45221, Northern Kentucky University, Highland Heights, KY 41099 and The Procter & Gamble Co., Cincinnati, OH 45253

The prognostic significance of nuclear accumulation of immuno-histochemically detected p53 in colorectal cancer (CRC) is controversial. In this study we compared, by the Student's t-test, the Area % epithelium: (1) binding lectins (Con A, UEA-1 and PNA following neuraminidase pretreatment); (2) expressing proliferating cell nuclear antigen (PCNA); (3) occupied by nuclei; or (4) reacting with galactose oxidase-Schiff's reagent (GOS) in 86 colorectal specimens that did or did not have nuclear accumulation of p53. Five year survival, tumor recurrence and T-antigen expression were compared to p53 expression by chi square analysis. We found that 79% and 73% of invasive and metastatic CRC specimens, respectively, and 40% of non-neoplastic mucosa and 41% of intraepithelial neoplasms had nuclear accumulation of p53. Neoplasms that expressed p53 were larger than p53 negative neoplasms. When all specimens were considered, p53 expression was not correlated with prognosis or T-antigen expression. However, invasive CRCs that were p53+ and later recurred had more tissue expressing PCNA. The Area % epithelium binding UEA-1 was reduced in metastatic CRCs with p53 expression and poor outcome. GOS reactivity was significantly increased in specimens with nuclear accumulation of p53. Since GOS reactivity may be a dosimeter of exposure to environmental/life style colorectal carcinogens (Carter et al, Validation of GOS, Clin. Can. Res. 3: 1479-89, 1997), this finding suggests that nuclear accumulation of immuohistochemically detected p53 expression in CRCs may reflect environmental factors.





This Article
Full Text (PDF)
Alert me when this article is cited
Alert me if a correction is posted
Services
Similar articles in this journal
Similar articles in PubMed
Alert me to new issues of the journal
Download to citation manager
Google Scholar
Articles by Carter, J. H.
Articles by Carter, H. W.
Articles citing this Article
PubMed
PubMed Citation
Articles by Carter, J. H.
Articles by Carter, H. W.


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]