Journal of Histochemistry and Cytochemistry, Vol. 45, 1673-1682, Copyright © 1997 by The Histochemical Society, Inc.


ARTICLE

The Secretory Granule and Pro-opiomelanocortin Processing in Xenopus Melanotrope Cells During Background Adaptation

Constance A.F.M. Berghsa, Shigeyasu Tanaka1,a, Frank J.C. Van Striena, Shingo Kurabuchib, and Eric W. Roubosa
a Department of Cellular Animal Physiology, Nijmegen Institute for Neurosciences, University of Nijmegen, Nijmegen, The Netherlands
b Department of Histology, The Nippon Dental University School of Dentistry, Chiyoda-ku, Tokyo, Japan

Correspondence to: Constance A.F.M. Berghs, Dept. of Cellular Animal Physiology, Nijmegen Institute for Neurosciences, Faculty of Science, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands.


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In this immunocytochemical study, we used light and electron microscopic observations in combination with morphometry to analyze the processing of pro-opiomelanocortin (POMC) in melanotrope cells of the intermediate pituitary of Xenopus laevis adapted to either a white or a black background. An antiserum was raised against a synthetic peptide including the cleavage site between ACTH and ß-lipotropic hormone in Xenopus. Western blotting revealed that this antiserum recognizes only a 38-kD protein, the POMC prohormone, from extracts of Xenopus neurointermediate pituitary. Light immunocytochemistry showed differential immunostaining for anti-POMC compared to anti-{alpha}-MSH. Anti-POMC was predominantly found in the perinuclear region, whereas anti-{alpha}-MSH yielded staining throughout the cytoplasm. Immunogold double labeling revealed that electron-dense secretory granules (DGs) show high immunoreactivity for anti-POMC and low immunoreactivity for anti-{alpha}-MSH. Electron-lucent granules (LGs) are immunoreactive to anti-{alpha}-MSH only. Moderately electron-dense granules (MGs) revealed intermediate reactivity compared to DGs and LGs. Background light intensity has significant effects on the morphology and the immunoreactivity of the secretory granules. Black-adapted animals have 4.5 times as many DGs and MGs as white-adapted animals. In addition, the MGs in black animals show 42% more anti-{alpha}-MSH immunogold than the MGs in white animals. Together, these findings indicate that the three granule types represent subsequent stages in granule maturation. Adaptation to a black background stimulates the formation of young immature granules, while at the same time the processing rate during granule maturation increases. (J Histochem Cytochem 45:1673-1682, 1997)

Key Words: pro-opiomelanocortin, {alpha}-MSH, melanotrope cells, quantitative immunoelectron, microscopy, Xenopus laevis


  Introduction
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The secretory granule is responsible for the storage and transport of secretory material from the Golgi apparatus to the cell membrane, where secretion takes place. Various studies have pointed out that, especially in endocrine and neuroendocrine cells, the secretory granule is not a static structure but is involved in many biochemical processes leading to the formation of bioactive messengers such as neuropeptides and neurohormones. These processes include proteolytic cleavage and enzymatic modification of a protein precursor, eventually allowing secretion of biologically active messengers. In the South African clawed toad Xenopus laevis, intragranular processing of the prohormone pro-opiomelanocortin (POMC) occurs in the melanotrope cells in the pituitary pars intermedia (Martens et al. 1980 ). These endocrine cells produce and release various POMC peptides, including {alpha}-MSH. When Xenopus is placed on a black background, {alpha}-MSH release stimulates pigment dispersion in dermal melanophores, thereby causing darkening of the skin. On a white background, {alpha}-MSH release is inhibited and the animal turns pale (for reviews see Jenks et al. 1993 ; Roubos et al. 1993 ). In the melanotropes, three morphologically distinct types of secretory granules have been identified: an electron-dense type (DG; mean diameter 130 nm) predominantly found in the vicinity of the Golgi apparatus; an electron-lucent type (LG; mean diameter 180 nm); and a type with moderate electron density (MG; mean diameter 160 nm). The population of secretory granules is strongly influenced by background light intensity. Whereas in white-adapted animals more than 90% of the granules are of the LG type, black-adapted animals show many DGs and MGs, forming together about one third of the total population (Roubos and Berghs 1993 ). Immunoelectron micro-scopy with antisera to {alpha}-MSH and to another POMC-peptide, {alpha},N-acetyl-ß-endorphin1-8 (van Strien et al. 1993 ), showed immunoreactivity of all three granule types, but significant granule type differences in labeling intensity with both antisera were found (Roubos and Berghs 1993 ). Whereas the DGs were only moderately stained, MG showed more labeling and the LGs were labeled most strongly. These labeling intensities depend on background light condition, because DGs, MGs, and LGs in black-adapted animals show higher labeling intensities than in white-adapted animals. On the basis of these data, it can be hypothesized that the three granule types represent morphological stages of the processing of POMC to mature POMC endproducts, including {alpha}-MSH, and that the regulation of these stages is under the influence of environmental light input.

The present study aimed to confirm this hypothesis by investigating whether different granule types represent different stages in granule maturation, whether processing of POMC in the secretory granules is a gradual process and occurs in parallel with the transition of DGs via MGs to LGs, and whether POMC processing is under the influence of background light intensity. For this purpose, a POMC antiserum was raised in this study against a synthetic peptide (ST-62) corresponding to the cleavage site between adrenocorticotropic hormone (ACTH) and ß-lipotropic hormone (ß-LPH) of Xenopus POMC. In rat melanotropes, the homologous sequence contains the first proteolytic cleavage site of murine POMC (Tooze et al. 1987 ; Tanaka et al. 1991 ; Tanaka and Kurosumi 1992 ). The antiserum was characterized using light microscopy and immunoblotting. Subsequently, the processing of POMC to {alpha}-MSH was investigated with quantitative immunoelectron microscopy, using the POMC antiserum and an antiserum to {alpha}-MSH.


  Materials and Methods
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Materials and Methods
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Production and Characterization of POMC Antiserum
Peptide synthesis was carried out according to a protocol described previously (see King et al. 1990 ; Tanaka et al. 1992). In short, a peptide (ST-62) including the cleavage site between ACTH and ß-LPH of Xenopus POMC (Glu-Leu-Arg-Arg-Glu-Leu-Ser-Leu-Glu-Cys; for amino acid sequence of Xenopus POMC, POMC, see Martens et al. 1985 ) was synthesized using an automated peptide synthesizer (Model 431A; Applied Biosystems, Foster City, CA). After purification of the peptide by reverse-phase HPLC, an antibody was raised in a guinea pig. The specificity of guinea pig anti-POMC was examined by preabsorption tests with the corresponding ST-62 peptide or with synthetic {alpha}-MSH (N-acetyl-{alpha}-MSH-NH2; Sigma, St Louis, MO) at concentrations of 1-100 mg/ml for 16 hr at 4C, before their immunocytochemical use. The specificity of the rabbit anti-{alpha}-MSH serum used in this study has been shown previously by noncompetitive binding and preabsorption tests, which showed that the antiserum specifically recognizes {alpha}-MSH (Tanaka and Kurosumi 1986 ).

Western Blotting
The specificity of the POMC antiserum was also tested in a Western blot. Neurointermediate lobes of black-adapted animals were homogenized in 100 µl sample buffer consisting of 62.5 mM Tris-HCl (pH 6.8), 12.5% glycerol, 1.25% SDS, 0.0125% bromophenol blue, and 2.5% ß-mercaptoethanol. Samples and markers (MW range 14-94 kD) were centrifuged (5 min at 13,000 rpm) and the supernatant was removed and boiled for 5 min. Samples of 20 µl were loaded on a 4% stacking gel and separated on a 15% SDS-PAGE separating gel at 200 V. The gel was blotted at 10 V in transfer buffer (25 mM Tris, 0.192 M glycine, 20% methanol) using a BioRad mini-protease II cell system (Richmond, CA). The nitrocellulose membrane (0.45 mM reinforced NCs; Schleicher and Schuell, Dassel, Germany) was washed in 0.01 M Tris-buffered saline (TBS, pH 7.6) and treated with block ace (5% bovine serum albumin in TBS containing 0.2% Tween 20; Sigma) for 15 min, washed again in TBS, and incubated with anti-POMC (1:10,000) in block ace for 16 hr at 4C. The NC membrane was subsequently washed in TBS containing 0.2% Tween 20 (TBST), incubated with second antiserum (donkey anti-guinea pig, 1:100; Jackson Immunoresearch Laboratories, Avondale, PA) in block ace for 1 hr at room temperature (RT), washed in TBST, and incubated with peroxidase conjugate (GP-PAP, 1:1000; Nordic, Tilburg, The Netherlands) in block ace for 1 hr at RT. Then the membrane was washed with 0.3 M MgCl2, TBST, and TBS for 10 min, immersed in ECL detection reagent (Amersham Life Sciences; Poole, UK) for 1 min and exposed to Hyperfilm (Kodak; Rochester, NY).

Animals
Adult Xenopus laevis were obtained from laboratory stock and fed trout pellets (Trouvit; Trouw, Putten, The Netherlands) once a week. The animals were kept under constant illumination and water temperature was 22C. Before the experiments, animals had been adapted to either a white or a black background for 3 weeks. The melanophore dispersion state was consistently 1.0 ± 0.1 for white adaptation and 4.9 ± 0.1 for black adaptation, according to the index of Hogben and Slome 1931 .

Light Immunocytochemistry
After decapitation and dissection, brains with pituitary glands were fixed for 2 days in Bouin-Hollande, dehydrated, and embedded in paraffin. Serial sagittal sections (5 µm) were mounted on gelatin-coated glass slides. Sections were deparaffinized and rinsed in distilled water and PBS (0.01 M sodium phosphate buffer, 0.14 M NaCl, 0.1% sodium azide, pH 7.5). Then the sections were treated with 1% bovine serum albumin (BSA) in PBS for 2 hr, guinea pig anti-POMC (1:10,000) or rabbit anti-{alpha}-MSH (1:5,000) for 16 hr, donkey anti-guinea pig or goat anti-rabbit antiserum (1:100; Nordic) for 2 hr, and finally with guinea pig and rabbit PAP complex (1:100; Nordic) for 90 min. After washing in PBS, sections were treated with 10 mg 3,3'-diaminobenzidine tetrahydrochloride (DAB; Sigma), 0.25% nickel ammonium sulfate and 0.005% H2O2 in 100 ml 0.05 M Tris-HCl buffer (pH 7.6) to reveal peroxidase activity. Then sections were dehydrated and mounted.

Freeze-substitution and Immunoelectron Microscopy
Animals were decapitated and freshly dissected neurointermediate lobes were fixed in 1% glutaraldehyde in 0.05 M sodium phosphate buffer (pH 7.4) for 16 hr. Then the lobes were treated with 0.2% borohydride and 0.4% glycine in the buffer for 15 min, and cryoprotected by immersion in a graded series of glycerol (10%-20%-30% in the buffer, 30 min per concentration). Lobes were rapidly frozen in liquid propane (-180C) and transferred to a precooled chamber (-90C) in a quick-freezing apparatus (Reichert-Jung; Vienna, Austria). Then they were freeze-substituted by methanol containing 0.5% uranyl acetate and warmed at a rate of 4C per hour to -45C. Embedding in Lowicryl HM20 resin (BioRad) was carried out in three steps with an increasing ratio of resin to methanol. Polymerization of Lowicryl was performed by UV irradiation (360 nm) at -45C for 16 hr and at 20C for another 24 hr. Before the double labeling procedures, immunogold single labeling was executed with both antisera to assess the specifity and intensity of the labeling. For immunogold double labeling, ultrathin pale gold sections were collected on Formvar-coated nickel grids and preincubated with 50 mM glycine in PBS for 10 min, rinsed three times with 0.2% gelatin in PBS, and incubated in 1% BSA in PBS for 15 min. Then sections were incubated with a mixture of guinea pig anti-POMC (1:15,000) and rabbit anti-{alpha}-MSH (1:10,000) for 16 hr, followed by a mixture of goat anti-guinea pig IgG-coated gold particles (10 nm) and goat anti-rabbit IgG-coated gold particles (15 nm) for 2 hr (Biocell; Cardiff, UK). After rinsing in PBS, sections were treated with 1% glutaraldehyde and contrasted with uranyl acetate and lead citrate.

Control Experiments
To test the specificity of the immunoreaction, incubation of sections in preabsorbed POMC and {alpha}-MSH antisera and immunostaining without use of a primary antiserum was executed. To examine crossreactivity of the immunogold products, the mixture of immunogold was used in single labeling experiments with both anti-POMC and anti-{alpha}-MSH. With anti-POMC, only goat anti-guinea pig immunogold (10-nm gold particles) was present, and with anti-{alpha}-MSH only goat anti-rabbit immunogold (15-nm gold particles) was found, indicating that crossreactivity between the species is absent.

Quantitative Evaluation and Statistics
For quantitative evaluation of the double labeling with anti-POMC and anti-{alpha}-MSH, three white- and four black-adapted animals were studied. Per animal, secretory granules were counted in at least 15 melanotrope cells and expressed as numbers of DGs, MGs and LGs per 100 µm2 cytoplasmic area. For each secretory granule, the numbers of 10-nm (POMC immunoreactivity) and 15-nm ({alpha}-MSH-immunoreactivity) gold particles were counted on photomicrographs (final magnification x 40,000), and expressed as numbers of gold particles per granule (Gp/G). A random selection procedure (animals, cells, granules) was maintained throughout the experiment. Data were analyzed with one-way analysis of variance (Bliss 1967 ) ({alpha} = 5%) followed by Duncan's multiple range test (Steel and Torrie 1960 ). The analysis was preceded by tests for joint assessment of normality (Shapiro and Wilk 1965 ) and homogeneity of variance (Bartlett's test; see Bliss 1967 ).


  Results
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Anti-ST-62 Recognizes POMC
To test the specificity of the ST-62 antiserum for Xenopus POMC, immunoblotting was carried out with neurointermediate lobes of black-adapted Xenopus. The antiserum appeared to recognize a protein with a molecular weight of 38 kD, corresponding to the POMC precursor. No other bands were detected with the ST-62 antiserum, which will be referred to below as anti-POMC (Figure 1).



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Figure 1. Western blot analysis obtained with the anti-ST-62 antiserum (Lane 2). Anti-ST-62 recognizes one band (38 kD) corresponding to glycosylated POMC. Lane 1 shows molecular weight markers.

Differential Immunostaining with Anti-POMC and Anti-{alpha}-MSH at the Light Microscopic Level
The intermediate lobe of the pituitary of Xenopus consists of over 95% melanotrope cells, which can readily be identified on the basis of their characteristic round shape. Immunocytochemistry with anti-POMC showed intense staining of the cytoplasm, particularly in the perinuclear region of the melanotropes (Figure 2A and Figure 2B, arrows). With anti-{alpha}-MSH, strong staining throughout the cytoplasm of all melanotrope cells was observed in both black- and white-adapted animals (Figure 2C). Preabsorption of the antiserum with ST-62 completely abolished staining, whereas preabsorption with anti-{alpha}-MSH did not affect staining (data not shown).



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Figure 2. Light micrographs of the pituitary of a black-adapted Xenopus immunostained using the PAP method for POMC (A,B) and {alpha}-MSH (C). Whereas anti-POMC is predominantly found in the perinuclear region of the melanotropes (arrows), anti-{alpha}-MSH gives strong staining throughout the cytoplasm. D, pars distalis; I, pars intermedia ; N, pars nervosa. Bars: A = 100 µm; B,C = 10 µm.

Immunogold Labeling of the Secretory Granules with Anti-POMC and Anti-{alpha}-MSH
Freeze-substitution fixation yielded adequate ultrastructural preservation of the neurointermediate lobe, revealing melanotrope cells, folliculostellate cells, and axons with varicosities and synaptic terminals. Melanotropes of black-adapted animals showed a highly developed rough endoplasmic reticulum (RER) and an extensive Golgi apparatus (Figure 3B and Figure 3C), in contrast to white-adapted animals, in which these cell organelles were almost absent (Figure 3A). With respect to secretory granules, DGs, MGs, and LGs occurred in both black- and white-adapted animals. In black-adapted animals, significantly more DGs and MGs per cytoplasmic area were present than in white-adapted animals (10.7 ± 1.39 DGs and 14.4 ± 2.79 MGs in black-adapted vs 2.4 ± 0.77 DGs and 3.8 ± 0.61 MGs in white-adapted animals; p<0.05) (Figure 5). In white-adapted animals, the granule population consisted almost exclusively (i.e., more than 90%) of LGs. Significantly more LGs were found in white-adapted animals than in black-adapted animals (340.0 ± 29.26 LGs in white-adapted vs 108.6 ± 27.60 LGs in black-adapted animals; p<0.05) (Figure 5).



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Figure 3. Electron micrographs of pars intermedia fixed by freeze-substitution, showing melanotrope cells of a white-adapted (A) and a black-adapted animal (B). Bars = 1 µm. Three types of secretory granules are present in the melanotropes, electron-dense granules (DGs, arrows), moderately electron-dense granules (MGs, arrowheads), and electron-lucent granules (LGs, asterisks). Melanotrope cell of a black-adapted animal shows strongly developed rough endoplasmic reticulum and Golgi apparatus (G). After immunodouble labeling with anti-POMC and anti-{alpha}-MSH, melanotrope cells of a white-adapted animal show few developed cell organelles but LGs that are tightly packed together. In a melanotrope cell of a black-adapted animal, dilated saccules of the Golgi apparatus (arrowhead) and DG budding from the trans-Golgi network (arrows) show exclusively anti-POMC immunogold (small gold particles; C). N, nucleus; M, mitochondrion. Bar = 0.25 µm.



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Figure 4. Double labeled DGs, MGs, and LGs in a melanotrope cell of a white-adapted (A,C,E) and a black-adapted animal (B,D,F). Labeling for anti-POMC (small gold particles) and anti-{alpha}-MSH (large gold particles) is differentially distributed over the granule types. DGs show many small particles and few large gold particles (A,B). DGs located near Golgi cisternae are strongly labeled with small gold particles (B). LGs show only large immunogold particles (E,F). The MGs show numbers of small and large gold particles that are intermediate between those of DGs and LGs (C,D). Bars = 0.05 µm.



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Figure 5. Mean numbers of DGs, MGs, and LGs in melanotrope cells of white-adapted (open bars) and black-adapted (closed bars) animals per 100 µm2 cytoplasmic area and SEM. Different superscripts on top of columns indicate that groups differ significantly (p<0.05).

Immunoelectron microscopy of freeze-substituted neurointermediate lobes with anti-POMC and anti-{alpha}-MSH showed specific immunostaining in both black-adapted and white-adapted animals. Strong double labeling was observed for secretory granules, whereas background labeling over cytoplasm, mitochondria, cell nuclei, and extracellular spaces with either of the antisera was barely present (Figure 3 and Figure 4).

For anti-POMC, the RER was negative but some immunogold labeling occurred in dilated cisternae of the Golgi apparatus (Figure 3C, arrowheads). With respect to granule types, a differential staining pattern with anti-POMC was found (Figure 3C and Figure 4A-4F). In both black-adapted and white-adapted animals, virtually all DGs (i.e., more than 98%) showed high labeling intensities, 7.1 ± 0.60 Gp/G in black-adapted and 6.6 ± 1.70 Gp/G in white-adapted animals (Figure 4A, Figure 4B, and Figure 6A). DGs that were located within or close to the Golgi apparatus were strongly immunopositive (Figure 4B). Between 10 and 20% of these DGs were seen budding off from the trans-Golgi network (Figure 3C). Around 80% of the MG showed immunolabeling with anti-POMC, but the labeling intensity was about one third lower than in the DGs (4.1 ± 0.66 Gp/G in black, 3.9 ± 0.20 Gp/G in white animals) (Figure 4C, Figure 4D, and Figure 6A). In both black-adapted and white-adapted animals, the LGs were almost devoid of immunogold labeling. Only about one in four granules was labeled and usually revealed only a single gold particle (0.3 ± 0.06 Gp/G in black-adapted, 0.3 ± 0.05 GP/G in white-adapted animals) (Figure 6A). Preabsorption of the antiserum with the ST-62 peptide and omission of the first antiserum completely abolished the immunostaining (data not shown).



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Figure 6. Labeling intensities for anti-POMC (A) and anti-{alpha}-MSH (B) in the immuno-double labeling expressed as mean numbers of gold particles and SEM (vertical bars) per dense (DG), moderately dense (MG), and lucent granules (LG) for white-adapted (open bars) and black-adapted animals (closed bars). Different superscripts on top of the columns indicate that groups differ significantly (p<0.05).

For anti-{alpha}-MSH, immunogold labeling was completely restricted to secretory granules. Virtually all granules were labeled (i.e., more than 98%), both in black-adapted and white-adapted animals (Figure 4A-F). The granule types obviously showed differential labeling, with a staining pattern that was completely opposite to that obtained with anti-POMC. Weakest labeling was found over DGs (2.4 ± 0.30 Gp/G in black-adapted, 2.3 ± 0.17 Gp/G in white-adapted animals) (Figure 6B), especially DGs in contact with or close to the Golgi apparatus. Background light intensity had a clear effect on the MG. Whereas in white-adapted animals MGs did not differ in immunogold labeling compared to DGs (2.2 ± 0.21 Gp/G), in black-adapted animals MGs showed much stronger reactivity, which was 42% above the labeling of DGs (3.9 ± 0.61 Gp/G; p<0.05) (Figure 6A). Both in black-adapted and in white-adapted animals, LGs were much more strongly labeled than MGs (+40% and +48%, respectively) (Figure 6).


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Specificity of the Antisera
The antisera used in this study were carefully tested by immunoblotting and preabsorption tests. For the anti-{alpha}-MSH serum, the high specificity for {alpha}-MSH has been shown previously (Tanaka and Kurosumi 1986 ). The anti-POMC serum was tested in preabsorption tests. They showed a total loss of immunoreactivity after addition of the ST-62 peptide but not after preabsorption with an excess of synthetic {alpha}-MSH. Moreover, Western blot showed that only one protein band of 38 kD reacts with the antiserum. This molecular weight corresponds to that of the precursor POMC in Xenopus (Martens et al. 1980 , Martens et al. 1985 ). These results demonstrate that the anti-POMC serum specifically recognizes the precursor protein POMC.

First Cleavage Site of POMC in Xenopus
The immunoblot data reveal that the anti-POMC serum used in this study recognizes only one protein, POMC itself. This means that the sequence (Glu-Leu-Arg-Arg-Glu-Leu-Ser-Leu-Glu-Cys) to which the antiserum had been raised contains the first proteolytic cleavage site of Xenopus POMC. Earlier studies showed that this sequence includes the first proteolytic site of rat POMC as well (Tooze et al. 1987 ; Tanaka and Kurosumi 1992 ).

Light Immunocytochemistry Reveals Different Locations of POMC and {alpha}-MSH
The light immunocytochemical data show a differential staining pattern with anti-POMC compared to that obtained with anti-{alpha}-MSH. Whereas POMC occurs particularly in the perinuclear region of the melanotropes, {alpha}-MSH is found throughout the cytoplasm of the melanotrope cell. This observation indicates that POMC is contained within the perinuclear RER, the Golgi apparatus, and/or in immature secretory granules lying near the Golgi apparatus, whereas mature {alpha}-MSH appears to be present in secretory granules dispersed throughout the cell. The ultrastructural double labeling experiment confirms this idea (see below).

Different Granule Types Represent Different Stages in Granule Maturation
The ultrastructural observations show that the RER in the melanotrope cells of Xenopus laevis does not stain with either of the two antisera. Because it is highly unlikely that the RER would not contain POMC, the reason for the absence of anti-POMC immunoreactivity may be that the POMC molecules are packed very tightly together so that they cannot be reached by the POMC antibodies. On the other hand, it may be that POMC is too low in concentration to be detected or that the POMC molecule has another conformation that makes it unrecognizable for the antibodies. Immunoreactivity to anti-POMC is present within Golgi cisternae, suggesting that the Golgi apparatus contains POMC in a less tightly packed form and that this POMC is not yet processed to {alpha}-MSH. As the ultrastructural study also shows, the Golgi forms small granules with an electron-dense content, previously defined as electron-dense granules (DGs; Roubos and Berghs 1993 ). The immunoelectron microscopic study reveals that DGs are strongly positive with the anti-POMC serum, especially those still attached to the Golgi apparatus, indicating that the DGs contain a high amount of POMC. In the DGs, relatively few POMC molecules are processed to {alpha}-MSH, because the DGs poorly react with the anti-{alpha}-MSH serum and hence appear to contain only a small amount of {alpha}-MSH. Therefore, we conclude that these granules represent the first stage of the melanotrope secretory granule. In contrast to DGs, LGs are never seen associated with the Golgi apparatus. As to their contents, the situation is opposite to that of the DGs. Their immunoreactivity to anti-POMC and anti-{alpha}-MSH indicates that LGs contain a very low amount of POMC but a high amount of {alpha}-MSH. Apparently, in this granule stage almost all POMC has been processed to form {alpha}-MSH. Therefore, they appear to represent a late step in secretory granule maturation. MGs show an intermediate picture, both in morphology and in immunoreactivity. The size of these granules, their degree of electron density, and their immunolabeling with anti-POMC and anti-{alpha}-MSH are all intermediate compared to the DGs and LGs. Therefore, the MGs appear to represent an intermediate, transient step in granule maturation, in between the DGs and LGs, in which POMC is only partially processed to {alpha}-MSH (and probably to other POMC endproducts). Apparently, POMC is gradually cleaved in the secretory granules, which simultaneously change their morphological characteristics. The first cleavage of POMC may start within the trans-Golgi network, whereas the last cleavage activity of POMC may take place within mature LGs, which eventually release their fully processed endproducts by exocytosis. Interesting in this respect would be the immunolocalization of endoproteases PC1 and PC2 in the secretory granule types (Braks et al. 1992 ). Recently, a co-localization of PC1 with POMC and with POMC peptides was demonstrated in secretory granules of AtT-20 cells, confirming the hypothesis of gradual POMC processing during granule maturation (Tanaka et al. 1997 ).

Effects of Background Adaptation on Granule Maturation and POMC Processing
It is well known that adaptation to a black background has a stimulatory effect on the biosynthesis of POMC (Martens et al. 1980 , Martens et al. 1982 ; Ayoubi et al. 1992 ). The present study shows that this process is reflected at the morphological level by the fact that DGs, and especially the DGs associated with the Golgi apparatus, are much more numerous in melanotrope cells of black-adapted animals than in melanotropes of white-adapted Xenopus. In addition, the MGs are affected by background light, as is clear from the greater number of these granules in black-adapted animals, indicating a higher turnover of DGs to MGs. Whereas the amounts of POMC and {alpha}-MSH per DG do not appear to be related to the state of background adaptation, for MGs, the situation is different. The immunoreactivity of MG to {alpha}-MSH is higher under black than under white background conditions, suggesting that the rate of processing of POMC to {alpha}-MSH in the MGs is increased in black-adapted animals. As to the LGs, melanotrope cells of animals adapted to a white background clearly contain more of these granules than cells of black-adapted toads. Because under white background conditions melanotropes do not or barely release {alpha}-MSH (Jenks et al. 1993 ), it must be concluded that these cells have accumulated LGs, containing fully processed peptides, during their 3-week adaptation stay on a white background. Data show that LGs of black- and white-adapted animals do not differ in immunoreactivity to {alpha}-MSH. Most likely, the secretory granules in animals on a white background have had time enough (i.e., 3 weeks) to process all POMC to {alpha}-MSH, catching up in this way with the more rapid production of {alpha}-MSH under black background conditions. After a longer period on a white background, Xenopus appears to be fully ready for a fast adaptive response to a black background stimulus, because its melanotrope cells are crowded with LGs that contain fully processed peptides.

In conclusion, the present study shows that POMC processing to POMC endproducts, including {alpha}-MSH in secretory granules of the melanotrope cell of Xenopus laevis, is a gradual process, which occurs in parallel with the morphological transition of DGs via MGs to LGs. The DGs are the immature granules and the LGs are the mature granules, where the MGs represent an intermediate stage between DGs and LGs. Adaptation to a black background stimulates both the formation of immature granules and the intragranular processing of POMC, which is concomitant with the transition between the granule types.


  Footnotes

1 Present address: Dept. of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan.


  Acknowledgments

Supported by grants from the EU HCM (ERBCHRXCT920017) and the NWO/MW-INSERM exchange program.

We thank Huub Geurts and Tony Coenen for technical assistance, and Ron Engels for animal care.

Received for publication February 11, 1997; accepted July 3, 1997.


  Literature Cited
Top
Summary
Introduction
Materials and Methods
Results
Discussion
Literature Cited

Ayoubi TAY, Jenks BG, Roubos EW, Martens GJM (1992) Transcriptional and posttranscriptional regulation of the proopiomelanocortin gene in the pars intermedia of the pituitary gland of Xenopus laevis. Endocrinology 130:3560-3566 [Abstract]

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Hogben L, Slome D (1931) The pigmentary effector system: the dual character of endocrine coordination in the amphibian colour change. Proc R Soc Lond [Biol] 108:10-53

Jenks BG, Leenders HJ, Martens GJM, Roubos EW (1993) Adaptation physiology: the functioning of pituitary melanotrope cells during background adaptation of the amphibian Xenopus laevis. Zool Sci 10:1-11 [Medline]

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