BRIEF REPORT |
Differential Intracellular Distributions of Inositol Trisphosphate and Ryanodine Receptors Within and Among Hematopoietic Cells
Departments of Ophthalmology and Visual Sciences (AC,HRP) and Microbiology and Immunology (HRP), The University of Michigan Medical School, Ann Arbor, Michigan
Correspondence to: Dr. Howard R. Petty, Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, 1000 Wall Street, Ann Arbor, MI 48105. E-mail: hpetty{at}umich.edu
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Summary |
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(J Histochem Cytochem 53:913916, 2005)
Key Words: calcium neutrophil monocyte lymphocyte ryanodine receptor inositol trisphosphate receptor
CALCIUM IS A PLURIPOTENT messenger in many cells, especially hematopoietic cells. In monocytes, macrophages, and neutrophils, Ca2+ signaling has been linked with adherence, phagocytosis, phagolysosomal fusion, and motility (Kindzelskii and Petty 2003) In mast cells, Ca2+ signaling plays a key role in degranulation-mediated histamine release (Duffy et al. 2003
). T-helper cell function uses Ca2+ signaling pathways to promote changes in cytokine gene expression. In cytotoxic T lymphocytes, Ca2+ signaling promotes the release of perforin-containing granules (Berke 1994
). These and many other signals are mediated by Ca2+ in hematopoietic cells. How Ca2+ manages to do all this has presented challenging theoretical and experimental problems in signal encoding and systems biology. One approach has been encoding and physiological demodulation of the amplitude and frequency of chemical oscillators (e.g., Olsen et al. 2003
). Another approach has been to employ high-speed microscopy to examine the spatial characteristics of traveling Ca2+ waves within living cells (Kindzelskii and Petty 2003
; Petty 2004
). We now hypothesize that Ca2+-mobilizing receptors (inositol 1,4,5-trisphosphate receptor [IP3R] and the ryanodine receptor [RyR]) are differentially distributed within leukocytes. Our results show that the intracellular distributions of IP3R and RyR may differ within a cell type and across leukocyte types, suggesting that IP3R and RyR distributions contribute to the properties of signals and their functional consequences.
Peripheral blood was collected from healthy donors in compliance with the guidelines of the University of Michigan Institutional Review Board for Human Subject Research. Leukocytes were isolated using Ficoll-Histopaque density gradient centrifugation (Sigma Chemical Co.; St Louis, MO), then washed with PBS. Isolated cells were allowed to adhere to cover slips for 15 min at 37C. Cells were fixed for fluorescence microscopy using either 20 min at room temperature with 3.7% paraformaldehyde (Sigma) or the dithiobis (succinimidyl propionate) (DSP) procedure, which provides superior retention of labile cellular filaments (Safiejko-Mroczka and Bell 1996). Briefly, cells were first fixed using DSP (1 µg/ml; Pierce, Rockford, IL) in HBSS for 10 min at room temperature. Cells were extracted using 100% ethanol, followed by 5 min incubation in stabilizing buffer (1 mM EGTA, 4% polyethylene glycol 8000, and 100 mM PIPES at pH 6.9). Samples were washed with PBS, fixed with 3.7% paraformaldehyde for 20 min, then washed again.
Antibodies were conjugated with FITC and tetramethylrhodamine isothiocyanate (TRITC) (Molecular Probes). Anti-IP3R antibody reacting the C-terminal cytoplasmic domain of all three subtypes of the receptor (Chemicon International; Temecula, CA) was conjugated to FITC. Anti-RyR-1,2 antibody (BIOMOL International; Plymouth Meeting, PA) was conjugated to TRITC. Cover slips with fixed leukocytes were washed in PBS, and then blocked for 30 min with 3% BSA (w/v; Sigma) in PBS at room temperature. After blocking, cells were labeled simultaneously with anti-IP3R and anti-RyR at dilutions of 1:20 and 1:50, respectively, in PBS for 30 min at room temperature. Cover slips were washed thoroughly with PBS.
Samples were imaged using a Ziess Axiovert 135 inverted microscope coupled to an intensified digital charge-coupled device camera (Qimaging; Barnaby, BC, Canada). Cover slips were observed using bright field and fluorescence microscopy. To detect FITC, optical filters with an excitation at 482 nm and emission at 530 nm were used. For TRITC detection, optical filters with excitation and emission at 535 nm 590 nm, respectively, were employed. Images were obtained using QCapture image acquisition software (QImaging) and were processed using MicroTome deconvolution software (Vaytek Inc; Fairfield, IA) with an approximate point spread function. Image-Pro Plus (Media Cybernetics; Silver Spring, MD) and Adobe Photoshop 7.0 (Adobe Systems; San Jose, CA) were used to prepare the images.
The IP3R's intercellular locations were determined by immunofluorescence staining of human neutrophils, monocytes, and lymphocytes. In monocytes, the IP3R was present as a diffuse, uniform pattern throughout the cytoplasm and nucleoplasm (Figure 1B) . In neutrophils, the IP3R was found throughout the cell, including the interior of the nucleus (Figure 1E). The pattern in lymphocytes was distinct from that of other leukocytes. As with neutrophils and monocytes, the IP3R was distributed throughout the entire cell (Figure 1J). However, these cells were not labeled with nonimmune serum. In contrast to neutrophils and monocytes, lymphocytes exhibited an area of bright intensity inside the nucleus that morphologically corresponded to the nucleolus (arrowhead, Figure 1J), with brightness decreasing toward the cell surface. This unexpected morphological feature is further illustrated in Figure 2 . This figure shows quantitative line profile analyses of IP3R labeling of lymphocytes. Enhanced labeling is associated with the nucleolus. Hence, the IP3R is heterogeneously distributed within and among leukocytes.
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For comparison, staining was performed in an identical manner on leukocytes fixed using a conventional paraformaldehyde procedure. Some alterations in cell morphology were evident using bright-field microscopy (Figures 1M, 1P, and 1U). Changes in the intracellular distributions of Ca2+-mobilizing receptors were examined. Although IP3R staining in monocytes was diffuse as in Figure 1B, it did not clearly extend to the cell surface (Figure 1N). RyR labeling became markedly clumped (Figure 1O). Neutrophil labeling was more dramatically disrupted. In comparing Figure 1Q with Figure 1E, it is clear that labile cytoskeletal structures, such as filopodia, are retained by the DSP protocol, but not paraformaldehyde fixation. IP3R labeling was not uniform. Conventionally fixed cells exhibited IP3R clustering and the location of the nucleus was more clearly defined (Figure 1R). Furthermore, the nucleus was less clearly defined after RyR labeling (arrow, Figure 1S) of paraformaldehyde-fixed cells. In comparing Figures 1R with 1S, there is little that varies. The most dramatic change occurs in the lymphocyte. IP3R and RyR expression (Figures 1J and 1K, respectively) appeared to be the same in paraformaldehyde-fixed cells, but the nature of the staining was quite different in comparison to DSP-fixed cells. Rather than a gradient in the IP3 label, the pattern was uniform, with the exception of several bright clusters around the outer edge, none of which obviously corresponded to the nucleolus (Figure 1V).
This study demonstrates differential trafficking of the IP3R and RyR in hematopoietic cells, which may contribute to an intracellular "wiring" diagram that underlies immune function. Previous studies from this laboratory using high-speed fluorescence microscopy have demonstrated a variety of intracellular Ca2+ signaling patterns (Kindzelskii and Petty 2003; Petty 2004
). The differential intracellular trafficking of the IP3R and RyR may be one factor contributing to these signal patterns. For example, the increased IP3R density associated with lymphocyte's nucleolus may contribute to the intense Ca2+ wave initiated near this site during antigen stimulation (Li Q, et al., unpublished data). We suggest that the differences in Ca2+ mobilizing receptor distribution are linked with cell types.
Previous studies are consistent with our findings. For example, the IP3R resides in both the cytoplasm and nucleus in other cell types (Echevarria et al. 2003; Huh and Yoo, 2003
). Sugiyama et al. (1994)
showed that the expression profiles of IP3R subtypes shows cell-specific variations among hematopoietic cell lines. Northern and dot blot analyses revealed that the expression profiles for each cell type changes during differentiation (Sugiyama et al. 1994
). RyR expression levels vary among cell types (Furuichi et al. 1994
). We propose that epigenetic differences in receptor localization among cell types influence spatiotemporal characteristics of traveling Ca2+ waves and signal targeting within cells. The interplay among these receptors may tailor a cell's signaling properties to facilitate intracellular communication.
We also illustrate the importance of preserving labile cellular filaments and, thereby, the integrity of other cell structures by fixation with DSP. Organelles such as the endoplasmic reticulum are attached to the cytoskeleton. When cells are fixed with conventional chemical means, cytoskeletal structures depolymerize, which may allow organelles to assume artifactual shapes and locations. Because the IP3R and RyR are found in the endoplasmic reticulum (Pozzan et al. 1994), the ER's preservation is vital in assessing IP3R and RyR distributions. By employing this technique, we showed that the intracellular distributions of IP3R and RyR varies among monocytes, neutrophils, and lymphocytes. By double-labeling cells with anti-IP3R and anti-RyR, we also found that their expression is markedly different in the same cell, which is true for all three cell types used. DSP fixation allows observations of intracellular receptors that are lost with conventional techniques. In contrast, we found that fixation with paraformaldehyde creates artifactual clumps of IP3R and RyR in lymphocytes and neutrophilspresumably because of disruption of microtubules. This finding suggests that maintaining the integrity of these structures by rapid DSP fixation may be especially important when labeling lymphocytes, because they are more dramatically affected than other cell types studied.
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Acknowledgments |
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Footnotes |
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Literature Cited |
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