Journal of Histochemistry and Cytochemistry, Vol. 50, 1647-1657, December 2002, Copyright © 2002, The Histochemical Society, Inc.


ARTICLE

The mt1 Melatonin Receptor and RORß Receptor Are Co-localized in Specific TSH-immunoreactive Cells in the Pars Tuberalis of the Rat Pituitary

Paul Klosena, Christele Bienvenua, Olivier Demarteaua, Hugues Dardentea, Hilda Guerreroa, Paul Péveta, and Mireille Masson–Péveta
a Neurobiologie des Rythmes, CNRS-UMR 7518, IFR 37, Université Louis Pasteur, Strasbourg, France

Correspondence to: Paul Klosen, Neurobiologie des Rythmes, CNRS-UMR 7518, 12, rue de l'Université, 67 000 Strasbourg, France. E-mail: klosen@neurochem.u-strasbg.fr


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The pars tuberalis (PT) of the pituitary represents an important target site for the time-pacing pineal hormone melatonin because it expresses a large number of mt1 receptors. Functional studies suggest that the PT mediates the seasonal effects of melatonin on prolactin (PRL) secretion. The aim of this study was the characterization of the phenotype of melatonin-responsive cells. Furthermore, we determined whether RORß, a retinoid orphan receptor present in the PT, was co-expressed in the same cells. We combined nonradioactive in situ hybridization (ISH) with hapten-labeled riboprobes for detection of the receptors and immunocytochemistry (ICC) for detection of {alpha}GSU ({alpha}-glycoprotein subunit), ßTSH, ßFSH, ßLH, GH, PRL, and ACTH. Expression of mt1 mRNA was found in small round cells, co-localized with {alpha}GSU and ßTSH. However, not all ßTSH-containing cells expressed mt1 mRNA. The distribution of mt1- and RORß-positive cells appeared to overlap, although more cells were labeled for RORß than for mt1. Gonadotrophs, as well as other pars distalis cell types, were never labeled for mt1 melatonin receptor. Therefore, this study identifies the "specific" cells of the PT as the mt1 melatonin receptor-expressing cells.

(J Histochem Cytochem 50:1647–1657, 2002)

Key Words: mt1 melatonin receptor, RORß receptor, pituitary, pars tuberalis, in situ hybridization, immunocytochemistry


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THE VERTEBRATE PITUITARY GLAND consists of three lobes: the anterior and intermediate lobes, containing hormone-secreting cells, and the posterior neural lobe, containing the axon projections of hypothalamic vasopressinergic and oxytocinergic neurons. The latter is connected to the brain by the pituitary stalk. The anterior lobe is subdivided into the pars distalis (PD), which contains the bulk of the hormone-secreting cells, and the pars tuberalis (PT), located rostrally to the PD and extending along the pituitary stalk up to the median eminence. This PT consists of PT-specific secretory cells expressing thyroid-stimulating hormone (TSH), folliculostellate cells, and pars distalis (PD) endocrine cells (expressing pituitary hormones) (Stoeckel et al. 1979 ; Rudolf et al. 1993 ; Lin et al. 1994 ; Sakai et al. 1999 ). To date, the precise physiological role of this structure is still unclear. In mammals, the PT appears to be a major neuroendocrine target for melatonin because it presents the highest density of melatonin receptors in the brain and endocrine tissues (Williams 1989 ; Masson-Pevet et al. 1994 ). Melatonin is a hormone secreted by the pineal gland during the night. Its daily nocturnal signal, which is under the control of the circadian clock located in the suprachiasmatic nuclei (SCN), provides the organism with both a circadian and a seasonal message (Klein and Moore 1979 ; Sumova et al. 1995 ). The PT appears to play a major role in mediating some seasonal effects of melatonin on the neuroendocrine system, such as the photoperiodic control of PRL secretion by lactotrophs of the PD (Lincoln and Clarke 1994 , Lincoln and Clarke 1995 ; Hazlerigg et al. 1996b ; Lincoln and Richardson 1998 ; Morgan 2000 ; Stirland et al. 2001 ). Therefore, the PT can be considered as part of the biological timing system. However, the mechanism by which the melatonin signal is decoded within the PT is still unknown.

High-affinity receptors for melatonin have been described using 2-iodo-melatonin as a ligand (Vakkuri et al. 1984 ). Two high-affinity receptors for melatonin have thus far been cloned in rodents: the mt1 (Mel1a) and the MT2 (Mel1b) receptor subtypes (Reppert et al. 1994 , Reppert et al. 1995 ; Roca et al. 1996 ) which belong to the seven-transmembrane domain, G-protein-coupled receptor family (Dubocovich et al. 1999 ). However, the mt1 receptor appears to be the highly dominant subtype in the PT because targeted disruption of its gene in mice leads to undetectable levels of 2-iodomelatonin binding (Liu et al. 1997 ), whereas cells transfected with the MT2 receptor gene bind this ligand (Dubocovich et al. 1997 ). Furthermore, the MT2 receptor subtype is naturally nonfunctional in the golden hamster, which is a highly photoperiodic species (Weaver et al. 1996 ). Therefore, the mt1 receptor subtype appears sufficient to drive the melatonin-induced photoperiodic response. Although many studies have been performed on the circadian and seasonal transcriptional regulation of mt1 receptors in the PT (Gauer et al. 1993 ; Guerrero et al. 2000 ; Schuster et al. 2001 ), the exact cell type expressing this receptor has not yet been identified. Therefore, one aim of this study was the identification of this cell type in the rat by combining the nonradioactive in situ hybridization (ISH) for the mt1 mRNA with immunocytochemistry (ICC) for the different pituitary hormones.

Retinoid orphan receptors (ROR{alpha}, -ß, and -{gamma}) are transcription factors that belong to the nuclear receptor superfamily and act as monomers at a single half-site (Carlberg and Wiesenberg 1995 ; Mangelsdorf and Evans 1995 ). ROR{alpha} is expressed mostly in peripheral tissues and in the cerebellum (Nakagawa et al. 1997 ; Dussault et al. 1998 ), while ROR{gamma} is present in skeletal muscle (Hirose et al. 1994 ). Interestingly, RORß is expressed only in the central nervous system and in the PT of the pituitary gland. Its expression is particularly strong in components of the mammalian timing system such as the retina, the pineal gland, the SCN, and the PT (Schaeren-Wiemers et al. 1997 ; Park et al. 1997 ; Andre et al. 1998 ), where it displays daily variations (Baler et al. 1996 ; Schaeren-Wiemers et al. 1997 ; personal observation). Melatonin was initially suggested as the endogenous ligand for these receptors (Becker-Andre et al. 1994 ; Wiesenberg et al. 1995 ), but the evidence supporting this hypothesis has since been questioned (Greiner et al. 1996 ; Hazlerigg et al. 1996a ; Schaeren-Wiemers et al. 1997 ; Bogan et al. 1998 ). Thus far, the cell type expressing this receptor in the PT has not been identified. Therefore, in addition to the characterization of the cells expressing the mt1 mRNA, this study aimed to investigate whether mt1 and RORß mRNAs could be co-expressed in the same cells in both the PT and PD.


  Materials and Methods
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Animals
Adult male and female Wistar rats were housed in a 12:12 light:dark cycle with food and water ad libitum. The animals were sacrificed during the light phase by transcardial perfusion under deep isoflurane anesthesia. Heparin (500 IU, Héparine Choay; Sanofi, Gentilly, France) was injected into the heart just before a PBS wash containing 0.02% diethylpyrocarbonate (DEPC; Sigma, St Quentin Fallavier, France), followed by the fixative (4% paraformaldehyde, 75 mM lysine, 10 mM sodium metaperiodate in 100 mM phosphate buffer, pH 7.4).

The brains were immediately dissected with the pituitary gland attached, blocked into 1-cm-thick slices, and postfixed in the fixative for 2 hr at 4C. They were then rinsed in 50% ethanol and embedded in polyethylene glycol as described by Klosen et al. 1993 . Four to 12-µm-thick coronal and sagittal serial sections were cut and were either collected on silane-coated slides or processed as floating sections.

All procedures used in animal experimentation complied with the French National law implementing the European Communities Council Directive 86/609/EEC. All efforts were made to minimize the number of animals used.

In Situ Hybridization
Hapten-labeled (digoxigenin, fluorescein, or biotin) riboprobes with alkaline phosphatase detection were used. Three independent mt1 cDNAs were used: a partial 468-bp rat mt1 cDNA (Reppert et al. 1994 ), a 1021-bp rat mt1 cDNA (Poirel et al. 2001 ), and a full-length mouse mt1 cDNA (Roca et al. 1996 ). RORß was detected using riboprobes transcribed from a 512-bp partial rat RORß cDNA (Becker-Andre et al. 1993 ). For single ISH, the probes were labeled with digoxigenin. For double ISH, melatonin receptor probes were labeled with digoxigenin and RORß probes were labeled with either fluorescein or biotin. Fluorescein- or biotin-labeled RORß probes were first validated in single ISH for both sense and antisense probes.

Sense and antisense riboprobes were transcribed in the presence of labeled nucleotides according to standard procedures. The length of the probes was confirmed by formaldehyde–MOPS agarose gel electrophoresis and Northern blotting. Non-hydrolyzed full-length probes were used to ensure maximal specificity.

Polyethylene glycol sections were postfixed for 10 min at room temperature (RT) in 4% phosphate-buffered formaldehyde, rinsed in PBS, and digested for 30 min at 37C with 1–8 µg/ml proteinase K (Roche; Meylan, France) in PBS. Proteinase K digestion was stopped with 2% phosphate-buffered formaldehyde for 5 min on ice. After three rinses in PBS for 10 min each, the sections were acetylated twice for 10 min in 100 mM triethanolamine, 0.25% acetic anhydride. After two 10-min rinses in PBS, the sections were equilibrated in 5 x SSC, 0.05% Tween-20 before hybridization.

Hybridization was performed for 40 hr at 54C with 200 ng/ml labeled sense or antisense probes in 50% formamide, 5 x SSC, 5 x Denhardt's solution, and 500 µg/ml salmon sperm DNA. After hybridization, the probe was washed off in 5 x SSC at RT for 10 min. Stringency rinses were performed six times for 10 min in 0.1 x SSC at 72C. In some experiments, the sections were also treated with RNase (20 µg/ml in 400 mM NaCl, 10 mM Tris-HCl, pH 7.5, 2.5 mM EDTA for 30 min at 37C) either before or after the stringency rinses.

Hapten-labeled bound probes were detected with alkaline phosphatase-labeled sheep anti-digoxigenin antibodies, sheep anti-fluorescein antibodies, or streptavidin (Roche). Alkaline phosphatase activity was detected with bromo-chloro-indolyl phosphate (BCIP) and nitroblue tetrazolium (NBT) in the presence of 5% polyvinyl alcohol (70,000–100,000 MW; Sigma) (De Block and Debrouwer 1993 ).

For double ISH experiments, both probes were hybridized simultaneously and detected sequentially using BCIP/NBT as the first chromogen. After this first detection, the first anti-hapten antibodies were eluted by incubating twice for 15 min in 100 mM glycine-HCl buffer, pH 2.2, containing 0.1% Tween-20 (Philippe and Pollock 1998 ). On control sections, the elution was confirmed by the absence of detectable phosphatase activity.

The second probe was detected with peroxidase-labeled streptavidin (Roche), amplified with biotinylated tyramide (TSA; NEN, Boston, MA), and visualized with alkaline phosphatase-labeled streptavidin and Fast Red/naphthol-AS-MX phosphate (Sigma) as chromogen in the presence of 4% polyvinyl alcohol. The Fast Red/naphthol-AS-MX phosphate detection procedure is 10- to 100-fold less sensitive than the NBT/BCIP procedure, thus requiring a previous tyramide amplification. Without this amplification no signal could be obtained with Fast Red for either melatonin receptor probes or RORß probes.

Immunocytochemistry
ICC was performed using antibodies (obtained from the NIDDK; Dr. F. Parlow) directed against the following pituitary hormones: rabbit anti-rat {alpha}-glycoprotein-subunit ({alpha}GSU), rabbit anti-rat ß-thyroid-stimulating hormone (ßTSH), rabbit anti-rat ß-follicle-stimulating hormone (ßFSH), rabbit anti-rat ß-luteinizing hormone (ßLH), guinea pig anti-rat growth hormone (GH), rabbit anti-rat prolactin (PRL), and rabbit anti-human adrenocorticotropin (ACTH). For further characterization, we also used a rabbit antibody to the transcription factor Pit-1 (Santa Cruz Technology; Santa Cruz, CA).

The primary antibodies were incubated overnight and detected using biotinylated secondary antibodies and streptavidin–peroxidase conjuate (Roche). Peroxidase activity was detected using amino-ethyl-carbazole (AEC; Sigma) as chromogen.

For combined ISH and ICC, the slides were first hybridized. After visualization of phosphatase activity with NBT/BCIP, the slides were rinsed and selected for double labeling. The slides were rinsed in 95% ethanol for 5 min to elute some background label and to shift the color of the in situ label to blue. The slides were then immunolabeled using the standard protocol described above, with AEC as the chromogen. The absence of interactions between the ISH and the ICC was verified by omission of the primary antibody in the ICC detection. Further tests included ICC on sections that were mock-hybridized to check for antigen inactivation or decloaking by the hybridization procedure.

For the combined ISH and double ICC, the first immunolabel was performed using nickel–diaminobenzidine as chromogen. The antibodies of the first immunolabel were then eluted with 100 mM glycine buffer (pH 2.2) containing 50 mM ß-mercaptoethanol to reduce the disulfide links between the light and the heavy chains of the immunoglobulins. After PBS rinses, the second antibody was incubated overnight and detected using the standard protocol described above, with AEC as the third chromogen. The elution of the first sequence of antibodies was verified on control sections by omission of the primary antibody in the second ICC detection sequence.


  Results
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mt1 Melatonin Receptor Expression
We detected mt1 melatonin receptor expression in the PT of the adenohypophysis, in accordance with previous studies using radioactive ISH (Fig 1A–1D and Fig 2A). All three mt1 probes provided the same labeling pattern, although optimal results were obtained with the 1021-bp rat probe and the full-length mouse probe. The specificity of the ISH was confirmed by the absence of label with sense probes (Fig 2C). Furthermore, the in situ label was obtained at high stringencies (0.1 x SSC, 72C) and resisted RNase digestion.



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Figure 1. Rostrocaudal series of sections through the pars tuberalis (A–D,G,H) and the zonula tuberalis of the pars distalis (E,F,K,L) of the rat, hybridized for mt1 melatonin receptor (A–F) or RORß receptor (G–L). The sections are from the same animal, at similar levels, although not adjacent. Both receptors display a similar distribution pattern in the pars and zonula tuberalis. Note that the labeled cells in the zonula tuberalis (E,F,K,L) are scattered along the ventral surface of the pars distalis. Bar = 250 µm.



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Figure 2. (A) Sagittal section of the medial eminence (me) and the pituitary gland of the rat, hybridized for mt1 melatonin receptor. Labeling is detectable along the PT and surrounds the pituitary stalk in the transition towards the pars distalis (pd). No label is detectable in the pars intermedia (pi). 3V, third ventricle. Bar = 250 µm. (B) PT cells located in the lateral area, labeled for mt1 melatonin receptor. Some cells (arrow) are devoid of labeling. (C) Adjacent section to B labeled with mt1 melatonin receptor sense probe. Asterisk indicates a blood vessel identifiable in both sections. (D) PT cells located in the medial area, labeled for mt1 melatonin receptor. In this area the PT is only one to two cells thick. (E) Zonula tuberalis cells, clustered close to portal vessels (pv) and displaying a slightly lighter label for mt1 melatonin receptor than PT cells. Bar = 25 µm.

The labeled cells were small and ovoid, with the nucleus usually located in one end of the cell (Fig 2B, Fig 2D, and Fig 2E). Labeling was restricted to the cytoplasm, although some cells also displayed labeling of a small point-like structure in the nucleus, probably the nucleolus (Fig 2E). Not all cells of the PT were labeled. Although the proportion of labeled cells was very high, especially in the rostral part of the PT, a few nonlabeled ovoid cells could be detected (Fig 2B). We did not observe labeling of small stellate cells, suggesting that only endocrine cells contained the mt1 melatonin receptor. No mt1 melatonin receptor hybridization could be detected in the walls of the blood vessels (Fig 2B and Fig 2E).

The mt1 melatonin receptor was also expressed in the rostral part of the PD along the ventral surface (Fig 1E and Fig 1F). These scattered cells appeared identical to those of the PT and were sometimes grouped into small clusters. On sagittal sections, mt1 melatonin receptor-expressing cells could be detected from the most rostral part of the PT to the ventral part of the PD (Fig 2A). However, the density of labeled cells appeared to decrease from the rostral to the caudal part, especially in the PD (Fig 2E). No difference was detected between animals sacrificed early or late in the light phase.

Co-expression with Pituitary Hormones
All pituitary hormone antibodies used in the study provided specific labeling of their corresponding cell populations in the PD. Only the anti-GH antibody appeared to crossreact slightly with pro-opiomelanocortin (POMC), as could be detected by a slight labeling of the pars intermedia, POMC-containing neurons and nerve fibers in the hypothalmus, and some slightly labeled corticotrophs in the PD (results not shown). No crossreactivity was observed for any of the other antibodies. Immunolabeling of mock-hybridized sections revealed no antigen inactivation nor decloaking for the antibodies tested. Only a reduction in the background label could be detected. Furthermore, no interference between the hybridization and the immunolabeling procedures could be detected.

The pituitary hormones detected in the PT were {alpha}GSU, ßTSH, ßFSH, and ßLH, in accordance with previous studies. No labeled cells were observed with antibodies to GH, PRL, or POMC. In {alpha}GSU-labeled PT sections, gonadotrophs were usually easily distinguished from TSH-expressing cells by their larger size. A large number of PT cells were immunolabeled and almost all of them were ßTSH/{alpha}GSU-positive cells, with the FSH/LH-positive gonadotrophs representing only a very small fraction of the PT cells. Furthermore, these gonadotrophs were irregularly distributed throughout the PT, especially from one rat to another.

Co-expression of mt1 melatonin receptor was observed only with ßTSH and {alpha}GSU (Fig 3A and Fig 3B). The mt1 mRNA was never detected in ßFSH-, or ßLH-positive cells (Fig 3C and Fig 3D). Not all ßTSH-positive cells in the PT showed co-expression with mt1 melatonin receptor (Fig 3B and Fig 3E–3G). The proportion of double-labeled cells decreased towards the caudal end of the PT, while in the rostral part of the PT almost all cells appeared double labeled. In the pituitary stalk, both ßTSH single-labeled and mt1/ßTSH double-labeled cells were often located around portal vessels (Fig 3G and Fig 3H).



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Figure 3. Co-localization of mt1 melatonin receptor (blue label) with pituitary hormones (red label). In the PT, mt1 melatonin receptor is co-localized with ßTSH (A) and {alpha}GSU (B). Some PT cells are labeled only for {alpha}GSU (arrows in B). No co-localization is observed for gonadotropins (C,D). The proportion of mt1/ßTSH double-labeled cells decreases from the rostral part (E) of the PT towards the caudal part (F). The specific cells of the PT are often clustered around portal blood vessels (G,H), where ßTSH single-labeled cells can also be observed (arrow in H). Bars: A,C = 10 µm; B,D,H = 20 µm.

In the PD, the same pattern of co-expression was observed. The mt1 melatonin receptor was present only in a subpopulation of {alpha}GSU- and ßTSH-positive cells (Fig 4A and Fig 4B). The morphology of the ßTSH-positive cells in the ventral part and the rest of the PD differed. Whereas in the ventral PD these cells were small ovoid cells identical to those of the PT, in the rest of the PD the ßTSH-positive cells were large, with an angular shape. Furthermore, the small ovoid mt1-positive cells did not express the transcription factor Pit-1, which was present in the large angular thyrotrophs. A third population consisted of some small ovoid TSH-positive cells, which were devoid of mt1 melatonin receptor labeling and which were located among the mt1/ßTSH double-labeled cells in the ventral PD. Pit-1 expression was completely absent from this ventral area of the PD (Fig 4A).



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Figure 4. (A) Zonula tuberalis of the PD. The cells co-expressing ßTSH (red label) and mt1 melatonin receptor (blue label), called "specific cells," are characterized by the absence of the transcription factor Pit-1 (gray nuclear label). Note the absence of Pit-1 expression in the ventral area of the PD corresponding to the zonula tuberalis. (B) PD. Normal thyrotrophs co-express ßTSH (red label) and Pit-1 (gray nuclear label) but do not express the mt1 melatonin receptor. Note the angular shape of the cells compared to the oval shape of the specific cells in A. The Pit-1 transcription factor is also expressed by other non-thyrotroph endocrine cells in the PD. (C) The RORß (blue label) is present in cells expressing ßTSH (red label) in the PD. (D) No co-expression is observed between RORß (blue label) and ßLH (red label) or ßFSH. (E) Cluster of zonula tuberalis cells expressing the RORß receptor. (F) Same section as E, showing co-expression of mt1 melatonin receptor (red fluorescent label) in the same cells expressing the RORß receptor. When superposed on E, the fluorescent granules in F do not coincide with the violet granules in E. Bars = 20 µm.

RORß Expression in the PT and PD
A robust and specific signal for RORß was obtained in the cortex, the suprachiasmatic nucleus, and the thalamus, as previously described by Schaeren-Wiemers et al. 1997 and Park et al. 1997 . As for the mt1 melatonin receptor, the specificity of the hybridization signal was confirmed by the absence of labeling by sense probes, the high stringency of the hybridization procedure, and the resistance to post-hybridization RNase digestion. Almost identical results were obtained with all three hapten labels, except that signal-to-noise ratios were optimal for digoxigenin-labeled probes.

In the pituitary, the RORß expression pattern was identical to that reported by previous studies using radioactive ISH (Park et al. 1997 ; Schaeren-Wiemers et al. 1997 ; Andre et al. 1998 ) (Fig 1G–1J). RORß expression was strongest in the PT. The labeled cells were small ovoid cells, with some nonlabeled cells scattered among them (Fig 4C and Fig 4D). The density of the RORß cells was highest in the rostral PT and decreased slightly towards the caudal part.

In the PD, some very slight RORß expression was detectable throughout the gland. Furthermore, slight labeling was seen in the pars intermedia. However, in the ventral part of the PD, scattered small ovoid cells showed strong hybridization with the RORß probe. Therefore, the distribution of strong RORß expression in the rat PT and PD was identical to that observed for the mt1 melatonin receptor.

The only pituitary hormones present in RORß-positive cells were ßTSH and {alpha}GSU (Fig 4C). As with the mt1 receptor, ßLH and ßFSH were absent from the RORß-expressing cells (Fig 4D).

Co-expression of mt1 melatonin receptor and RORß in the same cells of the PT and the PD was confirmed by hybridization of adjacent 4-µm sections (not shown) and by simultaneous double ISH (Fig 4D and Fig 4F). We were unable to ascertain whether all cells co-expressed both the mt1 melatonin receptor and the RORß receptor. The tyramide-amplified Fast Red detection procedure remained less sensitive than the direct NBT/BCIP, thus raising the possibility that the Fast Red procedure was unable to detect some cells that would be positive by the NBT/BCIP procedure. Nonetheless, the majority of the cells displayed labeling for both receptors.


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We have observed mt1 melatonin receptor expression in a subpopulation of endocrine cells in the PT of the rat. The distribution and morphology of these cells, as well as their content of {alpha}GSU and ßTSH, identify them as the "specific" cells of the PT (Gross 1983 ; Stoeckel and Porte 1984 ; Rudolf et al. 1993 ; Sakai et al. 1999 ). However not all the "specific" cells of the PT express the receptor. No mt1 receptor expression was observed in gonadotrophs or follicular cells of the PT.

In the rat, although "specific" cells from the PT express the same hormonal subunits as the classical PD thyrotrophs, both cell types present many morphological and functional differences. Large and angular in the PD, they are small and ovoid in the PT (Fig 4A and Fig 4B; Rudolf et al. 1993 ; Sakai et al. 1992 , Sakai et al. 1999 ). Moreover, the transcription factor Pit-1 implicated in ßTSH expression in the PD thyrotrophs is absent in the specific cells of the PT (Fig 4A and Fig 4B; Lin et al. 1994 ; Bockmann et al. 1997 ; Sakai et al. 1999 ). Bockmann et al. 1997 have established that PT cells possess neither TRH receptors nor thyroid hormone receptors. Therefore, TSH synthesis and release in these cells are not subject to the classical controls by TRH and T4. Furthermore, the mt1 melatonin receptor mRNA is highly expressed in a large number of PT "specific" cells located in the PT but also in the rostroventral part of the PD, known as the zonula tuberalis in the sheep (Skinner and Robinson 1995 ), whereas it is never present in the classical thyrotrophs from the PD. Therefore, although they express the same hormones, TSH-immunoreactive cells in the PT and in the PD are not physiologically regulated in the same way. Wittkowski et al. 1988 have shown that the TSH content of PT-specific cells is strongly reduced in Djungarian hamsters subjected to short days compared to long days. Because this effect is prevented by pinealectomy and can be mimicked by injections of melatonin, it appears that melatonin is a major regulator of TSH expression in the PT (Bockers et al. 1995 ; Bockmann et al. 1996 ). Our results suggest that these effects could be mediated through the mt1 receptor.

The best-established effect of melatonin in mammalian PT cells is inhibition of forskolin-induced cAMP synthesis (Morgan et al. 1995 ; Vanecek and Watanabe 1998 ). However, this inhibitory effect requires a previous stimulatory input because melatonin alone has no effect on basal cAMP levels. Recent work by von Gall et al. 2002 suggests that this stimulatory input might be adenosine through the adenosine A2b receptor. The nocturnal downregulation of cAMP levels by melatonin sensitizes the A2b receptor.

Melatonin also downregulates the phosphorylation of CREB in ovine PT cells (McNulty et al. 1994 ). The phosphorylation of CREB in ovine PT cells is regulated through both cAMP-dependent and cAMP-independent mechanisms (McNulty et al. 1996 ). The same authors have described in vitro a serum-derived factor that provides a cAMP-independent stimulation of CREB phosphorylation, which is sensitive to melatonin. The presence of CRE-like elements in the promoter of both the {alpha}GSU and ßTSH genes (Kim et al. 1993 ; Hashimoto et al. 2000 ) suggests that melatonin may downregulate their expression by the reduction of phosphoCREB levels during the night. However, no data are available on the nycthemeral regulation of either {alpha}GSU or ßTSH.

In the hamster PT, Messager et al. 1999 , Messager et al. 2000 , Messager et al. 2001 have shown that the photoperiod also regulates the amplitude of expression of Per1, an early-response gene also present in the SCN and part of the circadian clock molecular mechanism (Bae et al. 2001 ; Reppert and Weaver 2001 ; Zheng et al. 2001 ). Interestingly, E-Box binding motifs for bHLH transcription factors such as CLOCK and BMAL1, two other clock-related genes, have been found upstream of the {alpha}GSU gene (Jackson et al. 1995 ; Wood et al. 1999 ). Because several clock-related genes are expressed in the PT of rodents (Messager et al. 1999 ; and personal observation), it could be hypothesized that the PT contains a slave molecular oscillator that could be reset and synchronized to the environment lighting conditions directly by melatonin action. In such a case, clock genes would constitute a relay for melatonin in mediating output effects, such as acting on thyrotropins.

Our study also demonstrates that mt1 and RORß receptors are co-expressed in the same cells. Unpublished results obtained in our laboratory show that the daily variations in RORß mRNA expression in the rat PT depend on the presence of endogenous melatonin. Furthermore, melatonin has been shown to regulate the transcriptional activity of ROR{alpha} in MCF-7 breast cancer cells (Dai et al. 2001 ), which are known to express the mt1 melatonin receptor (Ram et al. 2000 ). Therefore, it is tempting to speculate that RORß mRNA expression is dependent on melatonin through mt1 melatonin receptors. Melatonin might thus control the transcriptional activity of RORß receptors, which might then control other downstream genes. However, thus far no response element for ROR{alpha} or -ß has been described or functionally evaluated in {alpha}GSU or ßTSH genes.

Our results clearly establish the TSH-containing cells as the major cellular target of endocrine regulation by melatonin in the PT. However, the specific cells of the PT constitute only a small fraction of the thryotrophs of the adenohypophysis. Therefore, the photoperiodic downregulation by melatonin of ßTSH expression, and possibly secretion, would affect only marginally the total pituitary output of ßTSH. Furthermore, no clear effect of either {alpha}GSU or ßTSH on the PD secretory activity has been demonstrated in the adult. Therefore, the identity of the endocrine-specific product of the PT, called tuberalin (Stoeckel et al. 1994 ; Morgan et al. 1996 ), regulated by melatonin receptors in the TSH-containing cells and responsible for the photoperiodic regulation of PRL secretion by the lactotrophs of the PD, remains to be identified.


  Acknowledgments

Supported in part by the Institut de Recherche International Servier.

We wish to thank Dr F. Parlow and the National Hormone and Pituitary Program of the NIDDK for the gift of the anti-hormone antibodies, and Drs S. Reppert and I. Wiesenberg for the gift of the mouse mt1 and rat RORß cDNAs, respectively. We thank Dr J.P. Loeffler for help with Pit-1 detection. The expert assistance of D. Bonn and A. Senser in animal husbandry is gratefully acknowledged.

Received for publication May 6, 2002; accepted July 3, 2002.


  Literature Cited
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Summary
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Materials and Methods
Results
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Literature Cited

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