ARTICLE |
Correspondence to: Paul L. Debbage, Inst. for Histology and Embryology, Leopold-Franzens-University, Müllerstr. 59, A-6020 Innsbruck, Austria.
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Summary |
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Intravital lectin perfusion was combined with computer-guided scanning digital microscopy to map the perfused elements of the vasculature in tumor-bearing mice. High-precision composite images (spatial precision 1.3 µm and optical resolution 1.5 µm) were generated to permit exact positioning, reconstruction, analysis, and mapping of entire tumor cross-sections (c. 1 cm in diameter). Collation of these mosaics with nuclear magnetic resonance maps in the same tumor plane identified sites of rapid contrast medium uptake as tumor blood vessels. Digitized imaging after intravital double labeling allowed polychromatic visualization of two different types of mismatched staining. First, simultaneous application of two lectins, each bearing a different fluorochrome, revealed organ-specific differential processing in the microvascular wall. Second, sequential application of two boluses of one lectin, bearing different fluorochromes successively, distinguished between double-labeled microvessels, representing efficiently perfused vascular segments, and single-labeled microvessels, with inefficient or intermittent perfusion. Intravital lectin perfusion images of blood vessels in the vital functional state thus highlighted biologically significant differences in vessel function and served as high-resolution adjuncts to MR imaging. (J Histochem Cytochem 46:627639, 1998)
Key Words: microvessels, tumor perfusion, intravital lectin histochemistry, ultrastructure, morphometry, NMR
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Introduction |
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BLOOD FLOW in and through tumors varies strongly from one microregion of the tumor to another and may fluctuate widely over time in a single microregion (
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Materials and Methods |
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Tumor Model
C3H mice from the breeding colony of the GSF Research Centre were used. They carried subcutaneous axillary transplants of the mouse mammary adenocarcinoma AT17 (
Lectins
The lectins HPA (Helix pomatia agglutinin), SNA (Sambucus nigra agglutinin), RCA (Ricinus communis agglutinin of 120 kD molecular weight), and MAA (Maackia amurensis agglutinin) were applied in histochemical procedures and in intravital perfusion studies as conjugates with biotin, with fluorescein isothiocyanate (FITC), or with tetramethyl rhodamine isothiocyanate (TRITC). The lectins HPA,WGA (wheat germ agglutinin from Triticum vulgaris), and RCA coupled to colloidal gold particles of 10-, 15-, or 20-nm diameter were used to visualize selected oligosaccharides by electron microscopy. Lectins were obtained from Sigma (Deisenhofen, Germany), from EY Laboratories (Medac; Hamburg, Germany), or Vector (via Camon Labor-Service; Wiesbaden, Germany). HPA binds specifically to -galactosides, RCA to ß-galactosides, SNA to
2,6-linked sialic acids, and MAA to
2,3-linked sialic acids; for further details of binding specificity refer to
Lectin Histochemistry of Fixed and Embedded Tissues
For light microscopy, tumors were fixed in formalin, dehydrated in ascending alcohols, cleared in xylol as intermedium, and embedded in Paraplast (Merck; Darmstadt, Germany). Dewaxed and rehydrated sections were treated for 30 min with 0.1% H2O2 in 30% ethanol to block endogenous peroxidases. Some sections were next incubated for 13 hr with 0.020.2 IU/ml sialidase (Type V from Clostridium perfringens; Sigma) in PBS, pH 6.0, at 37C. Buffer controls for this desialylation step were carried out as recommended by
For electron microscopy, mice bearing AT17 tumors were perfused transcardially with 4% paraformaldehyde plus 2.5% glutaraldehyde and the tumors were left overnight in one change of the same fixative at 4C. Slices 0.5 mm thick were cut by hand and rinsed at least four times in 1-hr changes of ammonium chloride 0.5% in PBS (pH 7.35). After dehydration in an ascending alcohol series at progressively lower temperatures, as described by
Intravital Lectin Histochemistry
Fluorochrome-conjugated lectins were applied at 0.5 mg in 0.25 ml buffered saline, corresponding to 20 mg/kg body weight, by injection into the tail vein of the narcotized mouse, injection requiring 1015 sec. In double-labeling experiments, each lectin was dissolved 0.5 mg in 0.25 ml, the two solutions mixed (total volume 0.5 ml), and the mixture injected into the tail vein. Some mice received injections of a mixture of 0.5 mg HPATRITC and 10 U of sialidase in a total volume of 0.5 ml saline. Circulation of the lectin-containing solution was terminated after a period of 60 sec15 min by removal of the organs for study. Tissues were dissected rapidly from the the mouse and snap-frozen in isopentane cooled in liquid nitrogen. When prolonged storage was required, they were held in darkness at -70C or in liquid nitrogen. Frozen sections were cut at 1030 µm and stored dry (unfixed) at -70C, or after fixation either in 4% formaldehyde in buffer or in acid alcohol. For viewing, sections were coverslipped with Moviol 4-88 (Hoechst; Frankfurt, Germany).
Spectrofluorometric Quantitation of Lectins in Blood
Samples of blood were obtained by cardiac puncture (first series) or by exsanguination (second series) 75 sec16 min after intravital application of 20 mg/kg body weight of fluorescein-labeled HPA. The relative intensity of fluorescein fluorescence (485 nm excitation, 512520 nm emission) in sample volumes of 0.5 ml blood serum diluted fivefold in isotonic saline was measured by absorptive spectrofluorometry with an LS50B Luminescence Spectrometer (Perkin Elmer; Neuried, Germany) and the results calibrated against lectinfluorescein conjugates diluted in isotonic saline to known concentrations in the range of 40250 µg/ml.
MR Methods
Before MRI, the animals were anesthetized by inhalation with isoflurane (Forene; Abbott, Wiesbaden, Germany) and a 24-G catheter (Introcan; B. Braun, Melsungen, Germany) was inserted into the tail vein. During the MR measurements, inhalation with isoflurane was continued and the temperature in the animal holder was kept constant at about 28C. MRI was performed on a 400-MHz MR system (DMX 400; Bruker, Karlsruhe, Germany). For calculation of the T1 maps, a Snapshot FLASH pulse sequence was implemented on the MR system. After one inversion pulse, 20 different T1-weighted Snapshot FLASH images were acquired within 2.5 sec. A field of view of 2530 mm provided an in-plane resolution of about 0.25 x 0.25 mm2; the slice thickness was 0.75 mm. From the Snapshot FLASH images, T1 relaxation time maps were calculated pixel by pixel using a method described by
Light Microscopic Mapping of Intravital Fluorescent Markers
Fluorescent labeling was detected with an Axiovert 100 microscope (Carl Zeiss; Jena, Germany) with x 10/0.30 and x 40/0.75 objectives, providing optical resolution close to 1.5 µm and 0.7 µm, respectively. FITC epifluorescence excited at 480 ± 20 nm from a mercury arc source was detected at 535 ± 25 nm (Chroma filters; Brattleboro, VT) with a Photometrics CH250A camera operating at -24C. TRITC epifluorescence excited at 535 ± 25 nm from a mercury source was detected at 610 ± 40 nm (Chroma filters). Alternatively, red and green channels for laser scan double epifluorescence were obtained by use of a heliumneon laser (HeNe 543 nm) filtered through BP488 or LP543 filters, respectively, with detection through BP515/565 or LP590 barrier filters, respectively. Images in each channel were digitized with Carl Zeiss LSM software (Version 3.85 Beta), fields of view 686 µm wide being scanned into 512 pixels, yielding a pixel size of 1.34 µm, and were stored as 8-bit TIFF files. Images of double-stained preparations were recorded in separate channels dedicated to red and green images and were viewed as monochromatic images or superimposed with a precision of 1 pixel to generate overlay images that coded double staining as yellow. Automated exposure at the CCD camera used in conjunction with lateral and vertical scanning displacements of the specimen by use of a Zeiss motor-driven stage steered by software modules custom-written in the current Zeiss language enabled automatic sequential recording of adjacent fields, generating mosaics that mapped specimen areas measured in cm2. After exposure of each microscopic field of view (x 10 objective), the stage was moved a precalculated distance for exposure of the next field, the stage displacement and CCD camera field of view being adjusted relative to one another to record adjacent fields of view with an overlap error not exceeding 2 pixels (2.7 µm). Each image was stored in computer memory, requiring approximately 0.25 MB in noncompressed form, for later analysis by morphometric techniques. The depth of detail available in such a reconstruction could not be presented on a monitor screen but was available for analysis and could be rendered visible by zooming into the required region of the field of view. After data reduction to generate mosaics imaging the entire tumour, e.g., from 18 x 18 contiguous microscopic fields, the smallest caliber microvessels (sinusoids of capillary caliber) were visualized as single pixels or clusters of two or three pixels.
Collation of MR Maps and Light Micrograph Mosaics
MR maps for collation were selected from the 51 T1 parameter maps acquired before and during IV injection of CM. Comparison of the 51 maps in the sequence revealed the CM flow patterns within the tumor and identified the earliest detectable sites of enhanced CM concentration in the tumour (in images 1418), representing sites at which CM entered the tumor. One of these early maps (14) was selected for collation with light microscopic mosaics.
To collate mosaic reconstructions of micrographic images with MR T1 parameter maps, it was necessary to stack and align several micrographic mosaics with one another and with the MR map, because the MR map visualized a slice 750 µm deep whereas the cryostat sections used to prepare the micrographic mosaics were only 1015 µm thick. To aid alignment, a plastic cannula was positioned in the rostrocaudal axis of each tumor before MRI and was left in position during MRI acquisition. The cannula, imaged with high contrast as a cross-sectioned profile in each MR map, was removed before cryostat sectioning of the tumor, leaving a clearly identifiable hole in the tissue to serve as an alignment guide in the micrographic mosaic. Internal landmarks (such as large vascular structures) guided the final exact collation (compare Figure 7 with Figure 9). The MR maps were collated in raw data form without application of filtering techniques. Micrographic mosaics were filtered by application of threshold values to eliminate weak signals resulting from noise, then collated as single reconstructed images without further processing.
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Results |
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AT17 tumors serially passaged SC in the axilla comprised many islands of epithelioid cells embedded in a fibrotic interstitium. Strands of large and small microvessels lay ensheathed in this collagen-rich interstitium, and only rarely did one of the narrower microvessels enter one of the nests of tumor cells. The microvessels branched irregularly, and wide- and narrow-caliber vessels appeared to be bundled together. In general, the thin walls of the microvessels consisted exclusively of the processes of endothelial cells. Accompanying cells, such as pericytes or myofibroblasts, were rare, and multilayered walls containing smooth muscle cells were not observed. The endothelia bore binding sites for lectins and could be labeled reliably and with high contrast by applying MAA or HPA to sections of tumors embedded routinely in paraffin (Figure 1). Ultrastructural examination of the AT17 tumor confirmed that the microvessels consist of simple thin-walled tubes constructed of endothelial cell processes, ensheathed in an extensive fibrotic interstitium (Figure 2). Postembedding lectin histochemistry revealed different ultrastructural patterns of binding for several lectins, with the common feature that some binding sites for each lectin were present on the apical surfaces of the endothelial cells. HPA bound both to the endothelial cytoplasm and to the apical cell surface, whereas WGA and RCA bound almost exclusively to the apical surface, with minor decoration of the endothelial basal cell membrane. After brief intravital perfusion with fluorochrome-conjugated lectins, a thin layer of bright fluorescence outlined the walls of both dilated and narrow microvessels. The fluorescent marker generally remained restricted to the microvessel wall, producing an image with high contrast in which the microvessels appeared brightly stained as tubular structures against an unstained dark background, because the interstitium and the tumor cells bound no lectin. Confocal laser scanning microscopy permitted optical focusing through intravitally labeled microvessels and reconstruction of them for binocular viewing (Figure 3).
Before intravital perfusion studies were performed, the one-pass circulation time of the mouse bloodstream was determined by IV application of Evans Blue, resulting in a generalized body stain within 15 sec. Therefore, injection of an adequate dose of lectin visualizes all microvessels accessible to rapid perfusion within a few passes of the bloodstream, i.e., within a circulation period of about 60 sec.
Targeting of lectins introduced into the bloodstream was examined by intravital perfusion with a lectin mixture consisting of equal parts of an FITC-labeled lectin and a TRITC-labeled lectin. This rapidly double labeled the microvessels in organs throughout the body, including microvessels in the tumor. The narrow-caliber microvessels in the tumor showed almost complete identity of staining for pairs of lectins (Figure 4A), whereas in wider-caliber microvessels some separation of the two lectins could be observed even within 60 sec after IV injection of the lectin mixture (Figure 4B). These results were not unique to the tumor. For example, the capillaries in the lung also exhibited identity of staining (Figure 4C), whereas the larger microvessels in lung tissue showed separation of lectin pairs (Figure 4D). In other organs also, 60-sec circulation time sufficed for endothelial processing and separation of lectin pairs, e.g., in both liver and spleen (data not shown). At 60 sec the fluorochrome marking the lectin HPA generally labeled the microvascular endothelia but also appeared as collections of small granules or as clouds basal to the endothelia (Figure 4A), whereas RCA and SNA bound only to the endothelia. All three lectins decorated a continuous layer of the microvessel wall in all organs throughout the body. In the tumor, RCA and SNA occasionally failed to label the entire length of a microvessel segment. HPA did not exhibit this reduced binding in the tumor, and in double-staining perfusions it bound to segments of microvessels that failed to bind RCA or SNA. The majority of the results reported here, demonstrating microvessels accessible to blood-borne substances in AT17 tumors, were obtained using HPA.
The perfusion dynamics of lectins introduced into the bloodstream were investigated in correlative studies with MR. Tumors exhibiting rapid uptake of CM required only brief intravital exposure to lectins (60 sec) to label their microvessels. Tumors with slow uptake of CM required longer intravital exposure to lectins (15 min) to label their microvessels. Intratumor regional heterogeneity in CM uptake was a prominent feature in most AT17 tumors, indicating that the tumors contain regions with efficient perfusion close to others with inefficient perfusion. To enable dynamic analysis of tumor microvasculature with regionally heterogenous rates of perfusion, intravital lectin perfusions were carried out to determine lectin clearance rate from the blood and from the microvessel wall and to check lectin receptor turnover rate at the endothelial surface. The concentrations of TRITC- or FITC-labeled HPA in the blood reached values close to the theoretical maximum of 250 µg/ml (0.5 mg HPAFITC distributed into approximately 2 ml blood in each mouse) during the first 2 postbolus min and fell to approximately half this concentration after 5 min and to approximately 30% after 15 min (Figure 5). A single measurement of FITC levels in the urine at 10 min postbolus revealed a high concentration (>900 µg/ml, volume not measured). Further trials showed that the lectinfluorochrome concentration in the blood after 15 min (4080 µg/ml after a bolus of 20 mg/kg) generated only faint labeling of the microvessel walls. In contrast to the efficient renal clearance from the blood, clearance of lectins from the microvessel wall in AT17 tumors was slow. Most microvessels remained strongly fluorescent 4 hr after injection of a bolus of fluorochrome-conjugated HPA. This long-lasting microvessel labeling resulting from injection of a single bolus of labeled lectin preserved a snapshot view of those microvessels accessible to blood-borne tracers around the moment of injection. To check whether this persistent labeling might be due to a low receptor turnover rate at the endothelial surface, a bolus of unlabeled HPA was injected, followed 5 min later by a bolus of TRITC-labeled HPA. In these experiments, the resulting fluorescence in microvessels in both the tumor and the liver, spleen, and kidney was not consistent with a low receptor turnover rate, providing evidence that fresh HPA binding sites were again available at the endothelial surface within 5 min.
Knowledge of these parameters made it possible to design intravital perfusion protocols capable of distinguishing efficiently from inefficiently perfused microvessels in the tumors. A typical protocol stipulated sequential perfusions, with IV injection of a bolus of HPATRITC followed by 15-min circulation and clearance time (in which the lectin was cleared from the blood but not from the microvessel walls), then injection of a bolus of HPAFITC and, 60 sec later, removal and snap-freezing of the tumor and several organs, including liver, kidney, spleen, lung, and intestine. Varying the protocol parameters established that in AT17 tumors wide-caliber microvessels and also many narrow microvessels were commonly accessible to the blood-borne HPA introduced in both boluses of the sequence (identity of staining in Figure 6), but that some regions of the tumors contained many narrow-caliber microvessels not accessible to blood-borne lectin introduced in one or other of the boluses (mismatch staining in Figure 6). This considerable regional microheterogeneity in perfusion dynamics was occasionally observed to involve one of the larger microvessels and the fine-caliber microvessels arising from it, with the consequence that an entire island of tumor cells was temporarily deprived of perfusion.
Intravital lectin histochemistry visualized perfused regions of the AT17 tumor microvasculature with high intensity and high contrast, allowing images of the microvessels to be recorded through low-power objective lenses, which were nonoptimal for epifluorescence microscopy because of the low intensity of fluorescence obtained by their use, but maximizing the area of tissue recorded in a single image. Comparison of results obtained with a range of objectives showed that use of the x 10 lens resulted in images that could be recorded by the CCD camera with adequate contrast and resolution (Figure 4 and Figure 6), and which could also be recorded with high mechanical positioning accuracy to allow subsequent reconstruction representing the tissue in the form of mosaics. Figure 7 Figure 8 Figure 9 collate an MR map of an AT17 tumor with micrograph mosaics in the same plane, using a common numbering system to denote the same site in each Figure. In Figure 7, which shows number 14 of a sequence of 51 T1 parameter maps, the sites of initial CM uptake into a 750-µm-thick slice of the tumor appear dark gray against the paler regions free of CM. Inspection of the sequence revealed the presence of flow patterns in the tumor slice (Figure 8). For two of these regional flows, a major source of the flow can be identified (numbered 1 and 8 in the figures), visible as sites of initial CM uptake in Figure 7 and explicable in terms of large-caliber blood vessels visible in Figure 9. Therefore, the site of CM enhancement 0.6 mm wide and labeled 1 in Figure 7 collates with a blood vessel 0.5 mm wide and labeled 1 in each of the images shown in Figure 9B and Figure 9C. This vessel was the earliest to show CM enhancement in the MR map sequence, with signal intensities reaching levels comparable to those seen in the blood in the heart ventricles (compare Figure 7). As shown in Figure 8, CM flowed from this source at 1, along the tumor periphery to the sites denoted 2 and 3, then centripetally to the site denoted 4, where the flow split into two branches, with apparent termination at the sites denoted 5a and 5b. These sites 15 are clearly evident in Figure 7, (although in later maps in the sequence the sites denoted 4 and 5 appear much more pronounced), and they correspond one to one with the vascular structures ranging between 0.05 mm and 0.5 mm in diameter and denoted 15 in the mosaics shown in Figure 9AC. The blood vessel shown at site 1 can be considered one of the major vessels supplying the entire tumor, because anatomic dissections of a number of AT17 tumors showed only two vessels of this caliber entering most AT17 tumors. Its flow supplying this slice of the tumor collates entirely with vascular structures in the micrograph mosaics, even though these mosaics comprise only three of the approximately 75 that would be required to fully reconstruct the complete depth of the MR map shown in Figure 7. Their spacing (120 µm) is close enough to reconstruct this particular flow, although together they sample only about one third of the depth of the MR map. A second major, complex regional flow originated at site 8, with peripheral flows towards sites 7/6 and 9, together with centripetal flows with apparent terminations at sites 10a and 10b (Figure 8). This flow system, clearly visible in the MR map (Figure 7), could be collated with large-caliber blood vessels denoted 7,8,9 in Figure 9AC, and with smaller-caliber vessels denoted 6 and 10 a and b in Figure 9AC. Other flows seen in the MR map sequence and denoted by non-numbered arrows in Figure 8 lay partially or totally outside the volume reconstructed by the three mosaics shown in Figure 9.
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Discussion |
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Lectins are suitable tools for intravital labeling of the vascular system, because dense concentrations of O-linked and N-linked glycans are available for binding at the apical surface of endothelial cells in most organs of all mammals (
High levels of intravital endothelial labeling were obtained in many organs, including liver, lung, kidney, spleen, and gut. Microvessels in brain and in the AT17 tumor were much less intensely labeled. One finding was unique to the AT17 tumor, however. This was the only structure in the mouse in which 60-sec intravital circulation of the lectin did not suffice to label all the microvessels. Because these microvessels were demonstrably competent to bind the lectins, it follows that they were not accessible to the lectins during the 60 sec allowed for circulation. Using these data in combination with the values we obtained for perfusion parameters as discussed above, we designed sequential lectin perfusion studies which confirmed the occurrence in the AT17 tumor of intermittent perfusion, as described in other tumors by
The same parameters noted above, determining lectin staining of microvessels in tumors, will also figure among those governing uptake and washout of IV applied paramagnetic CM imaged by MRI in dynamic studies (
The intravital lectin perfusion technique permits more extensive analysis than has been thus far noted. After the completion of fluorescence microscopy, the unfixed cryostat sections are available for further analysis. They can, for example, be further processed for immunohistochemical demonstration of antigens characteristic of tumors, of stages in the cell cycle, or of cytokines. Sites in the micrographic mosaic images of the vasculature can therefore be related to specific details of the underlying pathology, such as nests of raised mitotic index, or apoptosis or necrosis, or to cytokines mediating any local inflammatory response. As a result, intravital lectin perfusion renders it possible to correlate details seen in MR quantitative analysis with parameters of considerable tumor biological significance. It should be noted that in any such correlative analysis the size of the ROI invoked in the MR evaluation shrinks to a single voxel. The application of intravital lectin perfusion is not restricted to complementing MR analysis, however. This method is applicable to aiding in evaluation of data obtained by any of the imaging procedures used to study the living organism, e.g., positron emission tomography. It allows, in principle, the correlation of such data with local parameters that regulate blood flow.
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Acknowledgments |
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We thank Prof Dr M. Pavelka for her generous support and encouragement of this work and Dr J. Kummermehr for helpful discussion of the biology of the AT17 tumor. We thank Dr R. Seneckowicz for access to her mouse model of human amelanotic melanoma. We are grateful to Mr E. Mannweiler for developing the software modules, to Mr A. Voss for help with the spectrofluorometry, and to Ms S. Möllenstädt for care of the mice and transplanting the tumors and for assistance with the histochemistry. We thank Mr R. Haring and Ms J. Forgo for assistance with the electron microscopic histochemistry and with photography, and Dr C. Kremser for valuable help with preparation of the computer images. We are grateful to Schering (Berlin, Germany) for kindly providing gadolinum compounds.
Received for publication March 11, 1997; accepted November 20, 1997.
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