Journal of Histochemistry and Cytochemistry, Vol. 45, 1207-1216, Copyright © 1997 by The Histochemical Society, Inc.


ARTICLE

In Vivo Modulation of Connexins 43 and 26 of Human Epidermis by Topical Retinoic Acid Treatment

Elisabeth Masgrau-Peyaa, Denis Salomona, Jean-Hilaire Saurata, and Paolo Medab
a Department of Dermatology, University of Geneva, Geneva, Switzerland
b Department of Morphology, University of Geneva, Geneva, Switzerland

Correspondence to: Denis Salomon, Clinique de Dermatologie, Hôpital Cantonal Universitaire, CH-1211 Genève 4, Switzerland.


  Summary
Top
Summary
Introduction
Materials and Methods
Results
Discussion
Literature Cited

After 14 weeks of topical application of 0.1% all-trans-retinoic acid to the napes of volunteers, we observed a 2.5-fold increase in the thickness of epidermis, owing to an increase (p<0.001) in the number and size of keratinocytes and the induction of keratin 6. These changes in the differentiation of epidermal keratinocytes were paralleled by an increase in the amount of Cx43, a connexin that is normally expressed in human epidermis, and by the massive induction of Cx26, which is barely detectable in normal interfollicular epidermis, as judged at both the transcript (Northern blotting) and the protein level (immunolabeling). In contrast, retinoic acid treatment did not alter the morphology and connexin pattern of hair follicles or of sebaceous and sweat glands, and did not induce the expression of other connexins (C32, Cx37, Cx40) in either skin adnexae or epidermis. These observations suggest that the expression of two distinct connexins by interfollicular keratinocytes is related to selective changes in the differentiation program of epidermis that are induced by retinoic acid. (J Histochem Cytochem 45:1207-1215, 1997)

Key Words: gap junctions, connexins, all-trans-retinoic acid, epidermis, keratinocytes


  Introduction
Top
Summary
Introduction
Materials and Methods
Results
Discussion
Literature Cited

Keratinocytes of human skin are connected by gap junctions (Wolff and Schreiner 1968 ), the specialized domains of the cell membrane at which highly permeable channels for direct cell-to-cell exchange of current-carrying ions (Van Heulekom et al. 1972 ; Salomon et al. 1992 ), low molecular weight tracers (Salomon et al. 1988 ), and small metabolites (Hunter and Pitts 1981 ) are concentrated. Even though the role of gap junctions in keratinocyte function remains to be demonstrated, they have been proposed, by analogy to the roles contemplated in other systems (Lowenstein 1979 ; Lowenstein and Rose 1992 ), to be central to the regulation of keratinocyte growth and differentiation (Pitts and Finbow 1988; Watt 1992 ; Salomon et al. 1993 ; Brissette et al. 1994 ; Goliger and Paul 1995 ; Sawey et al. 1996 ).

This regulation is dependent on the interplay of a number of growth factors and morphogenetic molecules, among which all-trans-retinoic acid (RA) plays a prominent role (Fuchs 1990 ; Darmon 1991 ; Rosenthal et al. 1992 ). Junctional communications could mediate the biological effects of this natural messenger, because previous studies on primary human keratinocytes and derived cell lines have documented that exposure to RA involves changes in cell-to-cell coupling (Rogers et al. 1990 ; Salomon et al. 1992 ), in the number and size of gap junctions (Prutkin 1975 ; Elias et al. 1980 ), and in the expression of at least one of their constitutive connexin (Cx) proteins (Guo et al. 1992 ).

The gap junctions of human keratinocytes comprise at least two distinct connexins. Cx43 is abundantly expressed within the interfollicular epidermis (Guo et al. 1992 ; Wingelbus et al. 1992 ; Salomon et al. 1994 ), whereas Cx43 and Cx26 have been shown to be variably distributed within skin adnexae (Salomon et al. 1994 ). This distinct and partially overlapping distribution is believed to impart specific characteristics to the gap junctions of different skin regions, which presumably account for the differential patterns of communications that are observed between different subsets of human keratinocytes (Salomon et al. 1988 ; Kam and Hodgins 1992 ). In turn, it is assumed that these differential communications underlie different physiological functions of gap junctions in various skin regions.

A first objective of this study was therefore to investigate whether the two connexins that are normally expressed in normal skin are similarly affected by an RA treatment that markedly alters the differentiation program of human keratinocytes. Because such an alteration is known to be associated with the appearance of proteins that are not natively expressed by interfollicular keratinocytes, e.g., keratin K6 (Rosenthal et al. 1990 , Rosenthal et al. 1992 ), we have also assessed whether RA could induce the expression of connexins that are usually undetectable in control human skin.


  Materials and Methods
Top
Summary
Introduction
Materials and Methods
Results
Discussion
Literature Cited

RA Treatment and Tissue Sampling
The in vivo treatment was performed in agreement with the guidelines of our institutional committee for clinical investigation and with the informed consent of seven volunteers (four women and three men, 35-52 years old).

Each volunteer applied once a day, over 14 weeks, 0.2 ml of vehicle solution to an area of approximatively 6 cm2 on one side of the nape and a similar volume of a 0.1% all-trans-retinoic acid solution (Cilag AG Pharma; Schaffhausen, Switzerland) on the other side. Skin biopsies were taken in the center of treated and control areas under intra-dermal anesthesia with 1% lidocaine and 0.5 UI/ml Ornipressine (Sandoz; Basel, Switzerland). Samples were rapidly frozen by immersion in 2-methylbutane (Merck; Basel, Switzerland), which was cooled in liquid nitrogen, and stored at -80C until cryostat sectioning (for immunolabeling), or fixed at room temperature in 4% paraformaldehyde and 0.1% glutaraldehyde in PBS (for morphometric measurements).

For Northern blot analysis, three of the volunteers (one woman and two men, 35-39 years old) applied RA as indicated above on their buttock. This region was chosen because of its low density in hair follicles and the possibility of obtaining dermatome skin samples of constant thickness (0.3 mm) without esthetic prejudice.

Immunostaining
For immunofluorescence, sections about 5-µm thick were cut with a cryomicrotome (1720 digital MGW; Leitz, Ober-kochen, Germany), collected on gelatin-coated slides, and fixed for 3 min in -80C acetone. All slides were then rinsed in cold (4C) PBS containing 0.5% bovine serum albumin and were processed for indirect immunofluorescence staining.

For localization of gap junction proteins, sections were first incubated for 2 hr at room temperature with one of the following antibodies: (a) affinity-purified rabbit polyclonal antibodies against liver Cx32, diluted 1:100 (Dermietzel et al. 1984 ); (b) affinity purified rabbit polyclonal antibodies against residues 108-122 of liver Cx26, diluted 1:500 (Goliger and Paul 1995 ); (c) affinity-purified rabbit polyclonal antibodies against residues 101-119 of liver Cx26, diluted 1:100 (Kuraoka et al. 1993 ); (d) affinity-purified rabbit polyclonal antibodies against mouse liver carboxy terminal portion of Cx26 (Traub et al. 1989 ); (e) polyclonal rabbit antiserum against residues 252-271 of heart Cx43, diluted 1:100 (Beyer et al. 1989 ); (f) affinity-purified rabbit polyclonal antibodies against residues 314-322 of heart Cx43, diluted 1:100 (El Aoumari et al. 1990 ); (g) mouse monoclonal antibodies against 19 amino acids of the carboxy terminal portion of Cx43, diluted 1:500 (Zymed Laboratory; So. San Francisco, CA); (h) affinity-purified rabbit polyclonal antibodies against residues 313-330 of Cx40 (Bruzzone et al. 1993 ); or (i) affinity-purified rabbit polyclonal antibodies against residues 266-281 of Cx37 (Haefliger; submitted for publication). Sections were then rinsed in PBS and incubated for 1 hr at room temperature with either fluorescein-conjugated anti-rat or anti-rabbit antibodies, whichever applicable, diluted 1:200. After further rinsing, sections were stained with a 0.03% Evans Blue solution, covered with 0.02% paraphenylenediamine in PBS-glycerol (1:2, v/v), and photographed on a Axiophot microscope (Zeiss) fitted with filters for fluorescein detection.

A similar protocol was followed to localize keratin 6, using either an affinity-purified rabbit polyclonal serum, diluted 1:200, (kindly provided by Dr. E. Fuchs) or mouse monoclonal antibody D5/16B4 (Boehringer; Mannheim, Germany), diluted 1:50. Accordingly, the second incubation was performed with fluorescein-conjugated anti-rabbit or anti-mouse antibodies, diluted 1:200.

In all immunostaining experiments, negative controls included exposure of sections during the first incubation to either pre-immune serum, normal rabbit or mouse serum, or fluorescein-conjugated anti-rabbit or anti-mouse antibodies, whichever applicable. Cryosections of rat liver, pancreas, and heart and of mouse skin were incubated in parallel with the human samples and used as positive controls for the different anti-connexin antibodies tested.

Morphometric Evaluation
Sections of RA- and vehicle-treated samples from each volunteer were incubated with an identical batch of affinity-purified antibodies against either Cx43 or Cx26, to quantitate immunostaining within the interfollicular epidermis. To this end, color slides of each section, taken at a magnification of x157, were projected at a final magnification of x2440 on a graphics tablet connected to an IBM personal computer for area measurements and event counting. In each slide, we scored the number of keratinocytes and immuno-fluorescent spots and measured the area of epidermis. Data were collected both cumulatively for the entire epidermis (excluding the stratum corneum, which never showed specific immunolabeling) and, separately, in the basal layer of the tissue, in the uppermost layer of the granular region, and in one layer in the middle of the spinous region. Controls showed that the latter layer was representative of the entire spinous region, as far as Cx labeling was concerned (not shown). On the basis of these data, we calculated the number of fluorescent spots per layer of keratinocytes at different stages of differentiation.

Aldehyde-fixed skin from RA- and vehicle-treated samples was embedded in Lowicryl K4M resin and sectioned at about 1-µm thickness using a Reichert OM10 ultramicrotome. Sections were stained with methylene blue and photographed as described above. Color slides of each section were projected at a final magnification of x1840 and were used to evaluate the number of keratinocytes per epidermal field (defined as the area of epidermis photographed in each slide) and the mean area of keratinocyte profiles in the basal, spinous, and granular layers of interfollicular epidermis. All areas were measured by planimetry.

Data were analyzed by analysis of variance and compared by the median, the Kolmogorov-Smirnov, and the Mann-Whitney tests, using programs ANOVA and NPAR TESTS of the SPSS-X statistics package (SPSS; Chicago, IL).

Plasmid Constructions
Human connexin cDNAs for Cx43 (Fishman et al. 1990 ) and Cx26 (Lee et al. 1992 ) were subcloned in plasmid Bluescript II KS. Connexin anti-sense probes were synthesized by in vitro transcription of these cDNAs, which were then linearized with Xho I and EcoR I (for Cx43) and Hind III and EcoR I (for Cx26) restriction site enzymes (Boehringer).

Identification of Connexin Transcript
Samples were homogenized in 2.5 ml 0.1 M Tris-HCl, pH 7.4, containing 1.3 M ß-mercaptoethanol and 4 M guani-dium thiocyanate. After addition of solid CsCl (0.4 g/ml), the homogenate was layered on a 2-ml cushion of 5.7 M CsCl-0.1 M EDTA (pH 7.4) and centrifuged at 35,000 rpm at 20C for 20 hr. Pelleted RNA was resuspended in 300 ml 10 mM Tris-HCl, pH 8.1, suppplemented with 5 mM EDTA and 0.1% SDS, extracted twice with phenol-chloroform, precipated in ethanol, and resuspended in water. For Northern blots, total cellular RNA was denatured by 1 M glyoxal in 0.01 M phosphate buffer containing 50% DMSO, electrophoresed in a 1% agarose gel (5-10 µg total cellular RNA/lane), and transferred overnight onto nylon membranes (Hybond N; Amersham International, Poole, UK). Filters were exposed for 30 sec to 302-nm light and stained with methylene blue. Radiolabeled cRNAs were synthezised by in vitro DNA transcription in a Tris buffer supplemented with 0.05 mg/ml BSA, 16 mM DTT, 1 U RNAse inhibitor, 3 NTP with RNA polymerase. Filters were prehybridized for 2 hr at 65C in a 0.05 M Pipes-buffered (pH 6.8) solution of 50% formamide which was supplemented with 2 mM EDTA, 0.1% SDS, 0.1 mg/ml salmon sperm DNA, 0.8 M NaCl, and 2.5 x Denhardt's solution. Filters were then hybridized for 18 hr at 65C with 2.106 cpm/ml solution 32P-labeled probe, washed twice at 65C in 3 x SSC and 2 x Denhardt's solution, followed by three washings at 70C in 0.2 x SSC, 0.1% SDS, and 0.1% sodium pyrophosphate. Filters were then exposed to film (XAR-5; Eastman Kodak, Rochester, NY) between intensifying screens at -80C for 1 to 4 days.


  Results
Top
Summary
Introduction
Materials and Methods
Results
Discussion
Literature Cited

RA Treatment Affected the Differentiation of Interfollicular Keratinocytes
Compared to controls, all samples of RA-treated skin showed a striking increase in epidermal thickness, which was on average 2.5-fold (p<0.0001), as evaluated by epidermal area in both Lowicryl-embedded (Figure 1) and frozen skin samples (Table 1). Additional measurements showed that this change was due to an increase (p<0.0001) in both the number of keratinocytes per epidermal field and in the average area of their profiles (Table 1). These changes were observed in all layers of the epidermis except for the basal layer, and were prominent in the spinous layers. Results were similar in all samples irrespective of the age and sex of volunteers. By contrast, the morphology of epidermal adnexae appeared unaffected by the RA treatment. In full agreement with previous observations (Rosenthal et al. 1990 , Rosenthal et al. 1992 ), we could not detect keratin 6 (K6) in the interfollicular epidermis of control skin, but we readily observed it throughout the thickened epidermis of RA-treated regions. In both control and RA-treated skin, immunostaining for K6 was observed throughout the inner and outer root sheaths of hair follicles but not in the secretory cells of nearby sweat and sebaceous glands (not shown).



View larger version (147K):
[in this window]
[in a new window]
 
Figure 1. RA treatment increased the thickness of epidermis. (A) The skin of the control side of the nape showed normal epidermal morphology. (B) In contrast, skin exposed daily to 0.1% RA for 14 weeks showed a striking increase in epidermal thickness. This change was caused by an increase in the size and number of keratinocytes of spinous and granular layers and by moderate intercellular edema. Bar = 15 µm.


 
View this table:
[in this window]
[in a new window]
 
Table 1. Morphometric evaluation of interfollicular epidermisa

RA Treatment Modulated the Expression of Cx43
Immunostaining of cryosections for Cx43, using one affinity-purified and one monoclonal antibody, revealed this gap junction protein in the interfollicular epidermis of control and RA-treated samples (Figure 2). Qualitatively, the distribution of Cx43 appeared similar in all cases. In both control and RA-treated skin, immunolabeling of Cx43 was weak in the basal layer of keratinocytes, strong throughout the spinous layers, weak again in the granular layers, and absent in the stratum corneum (Figure 2). Quantitative comparison of sections that had all been incubated with the same affinity-purified serum (f in the list reported in the Material and Methods section), revealed a significant (p<0.05) increase in the total amount of Cx43 immunofluorescent spots (Figure 2). This change was essentially due to a larger (p<0.05) number of these spots over the keratinocytes of spinous layers (Figure 2). Northern blot analysis of comparable samples confirmed that these changes were paralleled by increased levels of Cx43 mRNA (Figure 4).



View larger version (102K):
[in this window]
[in a new window]
 
Figure 2. RA treatment modulated the expression of Cx43. (A) Exposure of control skin sections to antibodies against Cx43 (e in the list in Materials and Methods) resulted in spotty immunolabeling throughout the interfollicular epidermis. (B) Phase-contrast view of the section shown in A reveals that the Cx43 was sparsely distributed between basal keratinocytes, very abundant in keratinocytes of the spinous layers, reduced in the granular layers, and absent in the stratum corneum. (C) A similar distribution of Cx43 was observed in the interfollicular epidermis of RA-treated skin, even though the amount of immunolabeling appeared increased compared to controls (A). (D) Phase-contrast view of the section shown in C reveals that this change is most marked over keratinocytes of the spinous layers, which also predominately contributed to the increased thickness of RA-treated epidermis. Bar = 15 µm. (E) In control skin (open columns), immunolabeling for Cx43 was moderate over basal and granular keratinocytes and much more abundant over spinous keratinocytes. A similar pattern was observed after RA treatment (shaded columns), which slightly increased (p<0.05) the numerical density of immunofluorescent spots over spinous keratinocytes. Values are mean ± SEM number of fluorescent spots per keratinocyte. Values within or above columns are numbers of slides scored.




View larger version (200K):
[in this window]
[in a new window]
 
Figure 3. RA treatment induced the expression of Cx26. (A) Exposure of control skin sections to antibodies against Cx26 (d in the list in Materials and Methods) failed to immunostain membrane structures compatible with gap junction plaques within the basal and spinous layers of interfollicular epidermis. (B) Phase-contrast view of the section shown in A. (C) The same antibodies caused punctate labeling of most keratinocytes throughout the epidermis of RA-treated skin.This labeling was minute in the basal layer of the epidermis and very abundant in both spinous and granular layers. (D) Phase-contrast view of the section shown in C reveals that most of the Cx26 immunolabeling occurred over keratinocytes of the spinous layers, which predominantly contributed to the increased thickness of RA-treated epidermis. Bar = 15 µm. (E) Quantitative analysis revealed that immunolabeling for Cx26 was quite low throughout the epidermis of control skin (open columns). After RA treatment (shaded columns), the numerical density of Cx26 immunofluorescent spots markedly increased (p<0.0001) over both spinous and granular keratinocytes. Values are mean ± SEM number of fluorescent spots per keratinocyte. Values within or above columns are numbers of slides scored.



View larger version (61K):
[in this window]
[in a new window]
 
Figure 4. RA treatment increased differentially the expression of Cx43 and Cx26 transcripts. (Top) Northern blot analysis of total RNA (5 mg per lane) revealed the presence of a Cx43 transcript in all skin samples. The levels of this mRNA were notably greater in the retinoic acid-treated (RA) than in the control skin of each male (1) and female (2) volunteer tested. (Bottom) When the same RNA samples were tested for Cx26, a transcript encoding this connexin was detected in the retinoic acid-treated (RA) but not in the control skin (C) of every volunteer.

Analysis of skin adnexae failed to detect changes in their immunolabeling for Cx43. This connexin was detected in hair follicles, sebaceous glands, and ducts of eccrine sweat glands in both control and RA-treated skin samples (Table 2).


 
View this table:
[in this window]
[in a new window]
 
Table 2. Distribution of gap junction proteins in human skina

RA Treatment Induced the Expression of Cx26
Immunostaining of cryosections of control skin, using three different antibodies against Cx26, usually failed to detect this protein in interfollicular epidermis (Figure 3), except in some keratinocytes forming the upper granular layers. In contrast, when applied to sections of RA-treated skin, the same antibodies resulted in an abundant punctate staining of interfollicular keratinocytes of spinous and granular layers (Figure 3). Quantitative comparison of sections that had all been incubated with the same affinity-purified serum against Cx26 (d in the list reported in the Material and Methods section) revealed that this change predominated in keratinocytes of spinous and granular layers (p<0.0001) and was more modest in those of the basal layer (Figure 3). Northern blot analysis of comparable samples confirmed that the changes in Cx26 immunofluorescence were paralleled by a marked increase in the expression of Cx26 mRNA (Figure 4). Hence, whereas this transcript was not detectable in dermatome samples of normal skin, it was readily detected in samples of RA-treated skin from all the volunteers tested.

Contrasting with the major alteration observed within the interfollicular epidermis, no change in Cx26 immunolabeling was detected in skin adnexae. In both control and RA-treated skin, this connexin was readily detected in hair follicles and ducts of sweat glands, but not in sebaceous glands (Table 2).

RA Treatment Did not Induce Expression of Cx32, Cx37, and Cx40
Antibodies that identified Cx32, Cx37, and Cx40 in other tissues (human liver for Cx32, human heart for Cx37 and Cx40) failed to detect these gap junction proteins in the interfollicular epidermis of both control and RA-treated human skin (Table 2).


  Discussion
Top
Summary
Introduction
Materials and Methods
Results
Discussion
Literature Cited

Human keratinocytes are connected by many gap junctions (Salomon et al. 1988 , Salomon et al. 1993 ; Watt 1992 ; Wolff and Schreiner 1968 ). It is now established that these membrane domains allow direct cell-to-cell exchanges of multiple cytoplasmic constituents (Lowenstein and Rose 1992 ), but the role these exchanges may serve in skin remains uncertain. In the interfollicular epidermis of normal human skin, gap junctions and their major constitutive protein Cx43 are barely detectable between basal keratinocytes, sharply increase in the suprabasal layers where keratinocytes begin to acquire differentiated characteristics, decrease abruptly in the granular layers as differentiation progresses, and disappear in the uppermost layers of the epidermis, where full differentiation is achieved (Salomon et al. 1994 ). This heterogeneous distribution suggests that gap junctions may be involved in keratinocyte differentiation. To test this possibility, we have assessed whether connexin expression is affected by conditions that alter the differentiation program of adult epidermis. To this end, we have studied the effects of a long-term topical application of all-trans-retinoic acid, a treatment known to markedly modify the morphological and biochemical phenotype of keratinocytes (Rosenthal et al. 1990 , Rosenthal et al. 1992 ).

We have first confirmed that, in normal volunteers, this treatment induces a thickening of interfollicular epidermis (Rosenthal et al. 1990 , Rosenthal et al. 1992 ). Our quantitative analysis showed that this change resulted from both hyperplasia and hypertrophy of keratinocytes of spinous and granular layers. We also observed that these morphological alterations are associated with changes in the pattern of keratins synthesized by these cells (Rosenthal et al. 1990 , Rosenthal et al. 1992 ), as indicated by a massive expression of keratin 6. In humans, this protein is not detected in normal interfollicular epidermis but it is extensively induced in a variety of hyperproliferative skin diseases (Fuchs 1990 ) as well as after exposure to retinoids (Rosenthal et al. 1992 ).

Under these conditions of altered differentiation, we have first found that interfollicular keratinocytes increase by about 1.7-fold the expression of Cx43, a connexin that they natively express under control conditions. This increase was paralleled by moderate upregulation in the expression of Cx43 mRNA, analogous to that previously reported for another protocol of topical retinoid treatment (Guo et al. 1992 ). Our quantitative analysis reveals now that Cx43 increased specifically in the spinous layers of epidermis, in parallel with the increase in the size and number of differentiating keratinocytes, so as to maintain constant its immunoreactive amount per cell. In the very same samples we also found a much more pronounced increase in the expression of Cx26, which resulted in at least a 12-fold increase in the levels of this protein within the epidermis. At variance with what is seen in rodent skin (Risek et al. 1992 ; Kamibayashi et al. 1993 ; Butterweck et al. 1994 ; Goliger and Paul 1994 ; Sawey et al. 1996 ), Cx26 is not expressed in the basal and spinous layers of normal human epidermis (Guo et al. 1992 ; Salomon et al. 1994 ), although occasionally it is barely detectable between some keratinocytes of the granular layers. Quantitative analysis showed that after RA treatment Cx26 increased, mostly in keratinocytes of spinous and granular layers, i.e., in the same cells that also showed increased levels of Cx43. Previously, in human keratinocytes, the two connexins had been shown to coexist only in vitro (Dupont et al. 1993 ) in hair follicles and sweat ducts (Salomon et al. 1994 ) and in skin carcinomas (Wingelbus et al. 1992 ).

This finding shows that keratinocytes may express different connexins, not only depending on the species and the region of the epidermis but also as a function of the differentiation program of the tissue. Under the conditions studied here, differentiating keratinocytes of spinous and granular layers shifted their connexin pattern in vivo from the virtually sole expression of Cx43 to an increased expression of both Cx43 and Cx26. Clearly, the levels of these two connexins were not modified to the same extent. Hence, multiple connexins of a same keratinocyte subpopulation may be differentially regulated in vivo, as has been shown in vitro (Dupont et al. 1993 ). In different in vitro systems, Cx43 and Cx26 appear unable to interact with each other to form functional cell-to-cell channels (White et al. 1994 ). Therefore, their simultaneous expression by the same cell probably implies the formation of at least two distinct sets of gap junction channels. Because the channels made up of different connexins have distinct conductance and permeability characteristics (Bennett et al. 1993 ; Brissette et al. 1994 ), keratinocytes expressing two different connexins could modulate the gap junction-mediated exchange of ions and molecules much more precisely than keratinocytes that express a single connexin.

As yet, the signals crossing gap junctions in vivo remain undetermined, as is the biological function of their cell-to-cell passage. However, the present experiments indicate that a relationship should exist between the control of connexin expression and that of a specific differentiated phenotype. Therefore, chronic exposure to RA, which induces the keratinocytes of spinous and granular layers to enlarge and to express a keratin that is usually retricted to keratinocytes of skin adnexae (Heid et al. 1988 ), also induces the expression of Cx26, a connexin that is found between keratinocytes of skin adnexae. The differentiation program of keratinocytes comprising characteristics of epidermal adnexae may therefore require the exchange of signals through channels formed by Cx26.

The mechanism by which retinoic acid modulates the differentiation of the epidermis is still uncertain but appears to be related to selective changes in connexin expression (Guo et al. 1992 ), presumably resulting in a modulation of gap junction-mediated cell-to-cell communication (Pitts and Finbow 1986 ; Hossain et al. 1989 ; Mehta et al. 1989 ). Future studies should investigate the molecular link between the RA stimulus, the connexins, and the differentiation changes.


  Acknowledgments

Supported by grants from the Swiss National Science Foundation (32-42539.94 and 32-34086.95), the European Union (BMH4-CT96-1427), and the Research and Inventive Technologies Interstate Commission, Generalitat de Catalunya, Spain (BE 91-134 and BE 92-24).

We thank P. Carraux, A. Charollais, F. Cogne, J.-P. Gerber and E. Sutter for excellent technical assistance. We also thank Drs J. Goliger, Y. Shibata, O. Traub, and E. Beyer for providing us with anticonnexin antibodies, and Drs G. Fishman and S. Lee for human connexin cDNAs.

Received for publication January 2, 1997; accepted March 7, 1997.


  Literature Cited
Top
Summary
Introduction
Materials and Methods
Results
Discussion
Literature Cited

Bennett MV, Rubin JB, Bargiello TA, Verselis VK (1993) Structure-function studies of voltage sentitivity of connexins, the family of gap junctions forming proteins. Jpn J Physiol 43:10-18

Beyer EC, Kistler J, Paul DL, Goodenough DA (1989) Antisera directed against connexin 43 peptides react with a 43-kD protein localised to gap junctions in myocardium and other tissues. J Cell Biol 108:595-605 [Abstract]

Brissette J, Kumar N, Gilula N, Hall J, Dotto G (1994) Switch in gap junction protein expression is associated with selective changes in junctional permeability during keratinocyte differentiation. Proc Natl Acad Sci USA 91:6453-6457 [Abstract]

Bruzzone R, Haefliger JA, Gimlich RL, Paul DL (1993) Connexin 40, a component of gap junctions in vascular endothelium, is restricted with its ability to interact with other connexins. Mol Biol Cell 4:7-20 [Abstract]

Butterweck A, Elfgang C, Willecke K, Traub O (1994) Differential expression of the gap junction proteins connexin45, -43, -40, -31, and -26 in mouse skin. Eur J Cell Biol 65:152-163 [Medline]

Darmon M (1991) Retinoic acid in skin and epithelia. Semin Dev Biol 2:219-228

Dermietzel R, Leibstein A, Frixen U, Janssen-Timmen U, Traub O, Willecke K (1984) Gap junctions in several tissues share antigenic determinants with liver gap junctions. EMBO J 3:2261-2270 [Abstract]

Dupont E, Burra V, Madhukar BV, Trosko JE (1993) Suppression of gap-junction gene expression by growth factors and TPA in human epidermal keratinocytes in vitro. Prog Cell Res 3:321-327

El Aoumari A, Fromaget C, Dupont E, Reggio H, Durbec P, Briand JP, Boller K, Kreitman B, Gross D (1990) Conservation of a cytoplasmic carboxy-terminal domain of connexin 43, a gap junctional protein in mammal heart and brain. J Membr Biol 115:229-240 [Medline]

Elias PM, Grayson S, Caldwell TM, McNutt NS (1980) Gap junction proliferation in retinoic acid-treated human basal carcinoma. Lab Invest 42:469-474 [Medline]

Fishman GI, Spray D, Leinwand LA (1990) Molecular characterization and functional expression of the human cardiac gap junction channel. J Cell Biol 111:589-598 [Abstract]

Fuchs E (1990) Epidermal differentiation: the bare essentials. J Cell Biol 111:2807-2814 [Medline]

Goliger JA, Paul DL (1994) Expression of gap junction proteins Cx26, Cx31.1, Cx37, and Cx43 in developing and mature rat epidermis. Dev Dyn 200:1-13 [Medline]

Goliger JA, Paul DL (1995) Wounding alters epidermal connexin expression and gap junction-mediated intercellular communication. Mol Biol Cell 6:1491-1501 [Abstract]

Guo H, Acevado P, Parsa FD, Bertram JS (1992) Gap junctional protein connexin 43 is expressed in dermis and epidermis of human skin: differential modulation by retinoids. J Invest Dermatol 99:460-467 [Abstract]

Heid H, Moll I, Franke W (1988) Patterns of expression trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles. Differentiation 37:137-157 [Medline]

Hossain MZ, Wilkens LR, Mehta PP, Lowenstein WR, Bertram JS (1989) Enhancement of gap junctional communication by retinoids correlates with their ability to inhibit neoplastic tranformation. Carcinogenesis 10:1743-1748 [Abstract]

Hunter K, Pitts JD (1981) Non-selective junctional communication between some different mammalian cell types in primary culture. J Cell Sci 49:163-175 [Abstract]

Kam E, Hodgins MB (1992) Communication compartments in hair follicles and their applications in differentiative control. Development 114:389-393 [Abstract]

Kamibayashi Y, Oyamada M, Oyamada Y, Mori M (1993) Expression of gap junction proteins connexin 26 and Cx43 is modulated during differentiation of keratinocytes in newborn mouse epidermis. J Invest Dermatol 101:773-778 [Abstract]

Kuraoka A, Lida H, Hatae T, Shibata Y, Itoh M, Takashi K (1993) Localisation of gap juntion proteins, connexins 32 and 26, in rat and guinea pig liver as reveled by quick-freeze, deep-etch inmunoelectron microscopy. J Histochem Cytochem 41:971-980 [Abstract/Free Full Text]

Lee WL, Tomasetto C, Paul D, Keyomarsi K, Sager R (1992) Transcriptional downregulation of gap-junction proteins blocks junctional communication in human mammary cell lines. J Cell Biol 118:1213-1221 [Abstract]

Lowenstein WR (1979) Junctional intercellular communication and the control of growth. Biochim Biophys Acta 560:1-65 [Medline]

Lowenstein WR, Rose B (1992) The cell-cell channel in the control of growth. Semin Cell Biol 3:59-79 [Medline]

Mehta PP, Bertram JS, Lowenstein WR (1989) The action of retinoids on cellular growth correlate with their actions on gap junctional communication. J Cell Biol 108:1053-1065 [Abstract]

Pitts JD, Finbow ME (1986) The gap junction. J Cell Sci (suppl 4:239-266 [Medline]

Prutkin L (1975) Mucous metaplasia and gap junctions in the vitamin A acid-treated skin tumor, keratoacanthoma. Cancer Res 35:364-369 [Abstract]

Risek BF, Klier G, Gilula NB (1992) Multiple gap junction genes are utilised during rat skin and hair development. Development 116:639-651 [Abstract/Free Full Text]

Rogers M, Berestecky JM, Hossain MZ, Guo HM, Kadle R, Nicholson BJ, Bertram JS (1990) Retinoid-enhanced gap junctional communication is achieved by increased levels of connexin 43 mRNA and protein. Mol Carcinogen 3:335-343 [Medline]

Rosenthal DC, Griffiths EM, Yuspa S, Roop D, Vorhees J (1992) Acute or chronic topical retinoic acid treatment of human skin in vivo alters the expression of epidermal transglutaminase, lor-icrin, involucrin, filaggrin and keratins 6 and 13 but not keratins 1, 10 and 14. J Invest Dermatol 98:343-350 [Abstract]

Rosenthal D, Roop D, Huff C, Weiss J, Ellis C, Hamilton T, Vorhees J, Yuspa S (1990) Changes in photoaged human skin following topical application of all-trans retinoic acid. J Invest Dermatol 95:510-515 [Abstract]

Salomon D, Chanson M, Vischer S, Masgrau E, Vozzi C, Saurat J.-H, Spray DC, Meda P (1992) Gap junctional communication of primary human keratinocytes: characterisation by dual voltage clamp and dye transfer. Exp Cell Res 201:452-461 [Medline]

Salomon D, Masgrau E, Vischer S, Chanson M, Saurat JH, Spray DC, Meda P (1993) Gap junction proteins and communication in human epidermis. Prog Cell Res 3:225-231

Salomon D, Masgrau E, Vischer S, Ullrich S, Dupont E, Sappino P, Saurat JH, Meda P (1994) Topography of mammalian connexins in human skin. J Invest Dermatol 103:240-247 [Abstract]

Salomon D, Saurat JH, Meda P (1988) Cell-to-cell communication within intact human skin. J Clin Invest 82:248-254 [Medline]

Sawey MJ, Goldschmidt MH, Risek B, Gilula NB, Lo CW (1996) Perturbation in Cx43 and Cx26 gap junction expression in mouse skin hyperplasia and neoplasia. Mol Carcin 17:49-61 [Medline]

Traub O, Look J, Dermietzel R, Brummer F, Hülser D, Willecke K (1989) Comparative characterisation of the 21-kD and 26-kD gap junction proteins in murine liver and cultured hepatocytes. J Cell Biol 108:1039-1051 [Abstract]

Van Heulekom JS, Slaaf DW, Van der Leun JC (1972) Cell communications in the basal cells of human epidermis. Biophysics 12:1266-1284

Watt FM (1992) Intercellular communication via gap junctions. In Goldsmith L, ed. Physiology, Biochemistry and Molecular Biology of the Skin. Oxford, Oxford University Press, 857-869

White T, Bruzzone R, Wolfram S, Paul D, Goodenough DA (1994) Selective interactions among the multiple connexin proteins expressed in the vertebrae lens: the second extracellular domain is a determinant of compatibility between connexins. J Cell Biol 125:879-892 [Abstract]

Wingelbus KK, Kirkpatrick CJ, Knuechel R, Willecke K, Traub O (1992) Expression of Cx26, Cx32 and Cx43 gal junction proteins in normal and neoplastic human tissues. Int J Cancer 51:522-529 [Medline]

Wolff K, Schreiner E (1968) An electron microscopy study on the extraneous coat of keratinocytes and the intercellular space of the epidermis. J Invest Dermatol 51:418-430 [Medline]