Journal of Histochemistry and Cytochemistry, Vol. 45, 129-142, Copyright © 1997 by The Histochemical Society, Inc.


ARTICLE

Thymic Overexpression of CD40 Ligand Disrupts Normal Thymic Epithelial Organization

Robert J. Dunna, Carrie J. Luedeckera, Harald S. Haugenc, Christopher H. Cleggc, and Andrew G. Farra,b
a Department of Biological Structure, University of Washington, Seattle, Washington
b Department of Immunology, University of Washington, Seattle, Washington
c Bristol Myers/Squibb Pharmaceutical Research Institute, Seattle, Washington

Correspondence to: Andrew G. Farr, Dept. of Biological Structure, Box 357420, U. of Washington, Seattle, WA 98195-7420.


  Summary
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Materials and Methods
Results
Discussion
Literature Cited

We characterized the distribution of CD40 and CD40 ligand (CD40-L) in the adult and developing murine thymus. Before birth, CD40 was almost exclusively localized to scattered foci of medullary cells. By birth there was a dramatic upregulation of CD40 expression by cortical epithelial cells, which was accompanied by a consolidation of medullary epithelial foci. CD40-L+ thymocytes displayed a medullary location. Analysis of mice deficient in CD40-L expression indicated that CD40-L/CD40 interactions were not required for development of the medullary compartment. Overexpression of CD40-L targeted to thymocytes altered thymic architecture, as reflected by a dramatic loss of cortical epithelial cells, expansion of the medullary compartment, and extensive infiltration of the capsule with a mixture of CD3+ cells, B-cells, and macrophages/dendritic cells. Reconstitution of lethally irradiated normal mice with lck CD40-L bone marrow cells also resulted in loss of cortical epithelium and expansion of the medullary compartment. Disruption of the normal pattern of thymic architecture and epithelial differentiation as a consequence of increased intrathymic levels of CD40-L expression points to a role for CD40-L/CD40 interactions in the normal pattern of epithelial compartmentalization/differentiation within the thymic environment. (J Histochem Cytochem 45:129-141, 1996)

Key Words: Thymic epithelium, CD40-L, CD40, gp39


  Introduction
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Summary
Introduction
Materials and Methods
Results
Discussion
Literature Cited

The CD40 molecule is a 50-kd glycoprotein belonging to the TNF/NGF receptor superfamily (Mallett and Barclay 1991 ; Stamenkovic et al. 1989 ) which is expressed by B-cells and a variety of other cell types, including follicular dendritic cells, dendritic cells, endothelial cells, and thymic epithelial cells (Hollenbaugh et al. 1995 ; Galy and Spits 1992 ; Schreiver et al. 1989 ; Stamenkovic et al. 1989 ; Hart and McKenzie 1988 ; Clark and Ledbetter 1986 ). CD40 engagement by anti-CD40 monoclonal antibodies (MAbs) or CD40 ligand (CD40-L) affects B-cell activation and proliferation, B-cell selection/survival in germinal centers, and their differentiation to antibody-secreting plasma cells (Armitage et al. 1992 ; Hollenbaugh et al. 1992 ; Lederman et al. 1992 ; Noelle et al. 1992 ; Banchereau et al. 1991 ; Rousset et al. 1991 ), and expression of adhesion molecules by vascular endothelial cells (Hollenbaugh et al. 1995 ). Analyses of mutant mice deficient in either CD40 or CD40-L expression (Kawabe et al. 1994 ; Xu et al. 1994 ) have clearly established that interactions between CD40 and CD40-L play a critical role in T-cell-dependent B-cell activation.

The role of CD40 interactions in T-cell development is less clear. The absence of significant alterations of T-cell development in CD40null or CD40-Lnull mice suggests that CD40/CD40-L interactions are not absolutely required for T-cell development to occur (Kawabe et al. 1994 ; Xu et al. 1994 ). However, disruption of thymic negative selection in mice chronically treated with an anti-CD40-L MAb or in mice deficient in CD40-L expression suggests that CD40/CD40-L interactions may play a role in shaping the TCR repertoire (Foy et al. 1995 ). Overexpression of CD40-L perturbs normal thymocyte development and results in a lethal wasting disease with mononuclear infiltrates accumulating in multiple organs (Clegg CH et al., manuscript in preparation). Ligation of CD40 expressed by cultured human thymic epithelial cells affects cytokine production by these cells (Galy and Spits 1992 ) and, based on the impact of CD40 ligation on B-cell differentiation, might be expected to affect the differentiation of thymic epithelium as well.

In this study we characterized the expression of CD40 and CD40-L within the developing and adult normal murine thymus and assessed the effects of either the lack of CD40-L expression or elevated levels of CD40-L expression on the development and organization of the thymic environment. Although thymic tissue from mice lacking CD40-L displayed reduced expression of Class II MHC antigens and accessory molecules within the medullary compartment, the over all thymic architecture and phenotype was essentially normal. In contrast, thymic tissue from mice overexpressing CD40-L was profoundly affected, with B-cell infiltration/expansion, disorganization and loss of cortical epithelium, and dramatic increases of the medullary compartment.


  Materials and Methods
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Summary
Introduction
Materials and Methods
Results
Discussion
Literature Cited

Mice.
CD40-L-deficient mice produced by Xu et al. 1994 were obtained from Dr. Chris Wilson (University of Washington). Transgenic mice expressing CD40-L cDNA under control of the proximal lck promoter were produced at the Bristol Myers/Squibb Pharmaceutical Research Institute and will be described in more detail elsewhere (Clegg CH et al., manuscript in preparation). Radiation bone marrow chimeric mice were generated by reconstituting lethally {gamma}-irradiated (1000 rads) 6-week-old female C57Bl/6 mice with 107 bone marrow cells from female lckgp39 transgenic mice or syngenic female donors. The donor bone marrow cells were depleted of T-cells by treatment with anti-Thy1.2 MAb (clone 30-H12; American Type Culture Collection, Rockville, MD) and rabbit complement (Lo-Tox; Accurate Chemical and Scientific, Westbury, NY). Chimeric mice were analyzed 4-6 weeks after reconstitution.

Antibodies.
The following MAbs were obtained from the American Type Culture Collection: anti-CD4 (clone GK 1.5); anti-CD45 (clone RA3-3A1/6.1); anti-B7.2 (clone GL-1); anti-Class II MHC (clone M5/114.15.2); and anti-mouse CD11c (clone N418). The following MAbs were also used: anti-E-cadherin MAb [clone ECCD-2 (Takeichi 1990 )], anti-CD40-L MAb [clone MR-1 (Noelle et al. 1992 )], anti-B7-1 MAb [clone 1610A1 (Razi-Wolf et al. 1992 )], 6C3 (Adkins et al. 1988 ), ER-TR4 and ER-TR5 (Van Vliet et al. 1984 ). G8.8 and 10.1.1 were produced in this laboratory (Farr et al. 1991 , Farr et al. 1993 ). Anti-CD40 MAbs were purchased from Serotec (Kidlington, UK). Phycoerythrin-conjugated anti-CD8 MAbs and peroxidase-conjugated goat anti-rat Ig antibodies were purchased from CalTag (So. San Francisco, CA). RPE Cy5-conjugated streptavidin was from Southern Biotechnology (Birmingham, AL). Polyclonal goat anti-rat IgG antibodies were purchased from Pierce Chemical (Rockford, IL), and peroxidase-conjugated Fab fragments of sheep anti-digoxigenin antibodies were obtained from Boehringer Mannheim (Indianapolis, IN). Derivitization of MAbs with digoxigenin-3-O-methylcarbonyl-{epsilon}-aminocaproic acid N-hydrosuccinimide ester (Boehringer Mannheim) or d-biotinoyl-{epsilon}-aminocaproic acid N-hydroxy-succinimide ester (Pierce) was performed according to the manufacturer's instructions.

Light Microscopic Immunohistochemistry.
For light microscopy, frozen sections of thymic tissue embedded in OCT compound (Miles; Elkhart, IN) were mounted on aminoalkylsilane-subbed slides and allowed to air-dry for 2 hr before fixation in cold (-20°C) acetone for 20 min. After washing twice for 5 min in PBS, antigens were detected by either two- or three-step enzyme immunohistochemistry. For the two-step procedure, digoxigenin-conjugated MAbs were diluted in PBS containing 5% w/v nonfat dry milk and incubated with the fixed and hydrated tissue sections for 60 min at room temperature (RT). After washing three times for 5 min in PBS to remove unbound MAbs, peroxidase-conjugated Fab fragments of goat anti-digoxigenin were applied. The diluent for this conjugate was PBS containing 10% normal mouse serum (NMS). Enzyme activity was detected with a mixture of 3,3'-diaminobenzidine (DAB) and H2O2. The three-step procedure was similar, except that the primary MAbs were in the form of hybridoma supernatants or purified protein and the secondary MAb was digoxigenin-modified polyclonal goat anti-rat IgG antibody, which was detected with goat anti-digoxigenin antibody/enzyme conjugates. In some instances it was necessary to inhibit endogenous peroxidase before application of antibodies. Acetone-fixed tissue was incubated for 1 hr at 37°C in PBS containing 1 mM NaN3, 10 mM glucose, and 1 U/ml of glucose oxidase (US Biochemical; Cleveland, OH).

Electron Microscopic Immunohistochemistry.
Under ketamine/xylazine anesthesia, mice were fixed by cardiac perfusion with ~5 ml of HBSS, followed by ~30 ml of 0.1 M cacodylate buffer, pH 7.4, containing 4% paraformaldehyde and 1 mM CaCl2 (all at RT). Thymic tissue was then excised and cut into 50-µm-thick sections with a vibratome (Ted Pella; Tustin, CA). Tissue sections were incubated overnight at 4°C with primary antibodies diluted to 50 µg/ml in PBS containing 1% BSA and 5% sucrose. Sections were then washed three to five times with PBS/sucrose and incubated for about 6 hr with peroxidase-conjugated goat anti-rat IgG antibodies diluted to 30 µg/ml in PBS/BSA/sucrose. After repeated washing in PBS/sucrose, the sections were immersed for 15 min in PBS containing 1% glutaraldehyde, washed extensively with PBS/sucrose, and then immersed for 15-20 min in 0.05 M Tris buffer, pH 7.6, containing 0.0058% H2O2, 0.2 mg/ml DAB, and 5% sucrose. After additional washing in PBS/sucrose, the sections were immersed in 0.1 M cacodylate buffer, pH 7.4, containing 1% OsO4. After additional washing in distilled water, the sections were then prepared for conventional transmission electron microscopy.

Cell Culture.
Thymocytes at 107 cells /ml were cultured in RPMI 1640 containing 10% FBS, 5 x 10-5 M ß2-mercaptoethanol, penicillin (100 U/ml), and streptomycin (100 µg/ml) (CM). CM in some cultures also contained ionomycin (120 ng/ml) and PMA (12 ng/ml). After 1 hr at 37°C in a humidified environment containing 5% CO2, the cells were recovered and washed by centrifugation, then processed for flow cyometry. Aliquots of the starting cell population were also taken for flow cytometric analyses.

Flow Cytometry.
One million cells were incubated for 1 hr at 4°C with antibodies used at concentrations previously d etermined to yield optimal labeling. With direct fluorochrome-conjugated antibodies or fluorochrome-labeled anti-digoxigenin antibodies used in conjunction with digoxigenin-conjugated primary antibodies, the diluent contained 2.4G2 antibodies (Unkeless 1979 ) to minimize Fc-mediated binding of antibodies. Normal mouse serum (10% v/v) was added to the diluent with fluorochome conjugates of anti-Ig antibodies. Immediately before analysis of single- and two-color samples, 7-amino-actinomycin D was added as a viability marker (Schmid et al. 1992 ) and analyzed in the FL3 channel. Three-color analysis utilized biotinylated GK 1.5 in conjunction with streptavidin-RPE Cy5, PE-conjugated anti-CD8, and digoxigenin-modified MR-1 in conjunction with FITC-anti-digoxigenin antibodies. Flow cytometric data were obtained with a FACScan instrument (Becton Dickinson; Mountain View, CA) and analyzed with Reproman software (True Facts; Seattle, WA). At least 10,000 events were collected for each sample.


  Results
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Summary
Introduction
Materials and Methods
Results
Discussion
Literature Cited

Thymic CD40 Expression
In adult thymic tissue, staining with anti-CD40 MAb revealed a reticular pattern in the cortex and a more heavily labeled confluent pattern in the medulla (Figure 1a). Adjacent sections labeled with ER-TR5 and ER-TR4, which stain medullary and cortical TE, respectively (Van Vliet et al. 1984 ), confirmed that the confluent areas of CD40 expression were associated with the medullary compartment (data not shown). The pattern of CD40 expression was similar to that of Class II MHC (Figure 1b) and closely resembled CD40 expression described in the human thymus (Galy and Spits 1992 ).



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Figure 1. Thymic expression of CD40. (a) CD40 displays a delicate reticular pattern in the cortex (C) and a more confluent pattern in the medulla (M). (b) Class II MHC expression is qualitatively similar to that of CD40. (c) Labeling with an irrelevant rat MAb. Endogenous peroxidase is indicated by arrowheads; no blocking of endogenous peroxidase activity. (d) Immunoelectron microscopy of CD40 expression by cortical epithelial cells. Note the patchy labeling along the epithelial cell process (arrowheads). (e) Immunoelectron microscopy of a dendritic cell (DC) and epithelial cell (EP) in the medullary area. Epithelial characterization is based on the presence of distinctive cytoplasmic vacuoles with microvilli (arrowhead) and desmosomes (arrow). (f) Immunoelectron microscopy of a dendritic cell (DC) and epithelial cell (EP) in the medullary area. Epithelial characterization is based on the presence of distinctive cytoplasmic vacuoles with microvilli (arrowhead) and desmosomes (arrow). Bars: a-c = 100 µm; d-f = 1 µm.

Ultrastructural immunohistochemistry confirmed that the CD40 expression in the thymic cortex was associated with epithelial cells (Figure 1d). For the most part, there was a delicate linear distribution of the CD40 reaction product along the epithelial cell processes, which was consistent with the relatively light labeling of cortical CD40 at the light microscopic level. Interestingly, there were small segments of the epithelial cell processes that bore larger amounts of the reaction product, resulting in a patchy labeling pattern. In the medullary compartment, dendritic cells and epithelial cells, identified by ultrastructural features, were labeled with anti-CD40 MAb (Figure 1e and Figure 1f). In contrast to dendritic cells, which appeared to uniformly label well with anti-CD40 MAb, some medullary epithelial cells displayed very little reaction product (Figure 1e), whereas the labeling of others approached that displayed by dendritic cells (Figure 1f).

Thymocyte Expression of CD40-L
Previous flow cytometric analyses of normal young adult Balb/c thymocytes demonstrated low levels of CD40-L expressed by a small population of thymocytes, which increased following exposure to ConA or anti-CD3 MAbs (Nelson et al. 1996 ). Three-color flow cytometry was employed to examine thymocyte expression of CD40-L more closely. As shown in Figure 2a, labeling of normal thymocytes with MR-1 MAb resulted in a shoulder of dully stained cells. Using a channel number marker established with an irrelevant digoxigenin-modified primary MAb, about 9% of the thymocytes were MR1+, although the frequency of thymocytes expressing lower levels of MR-1 would be higher. In three-color analyses of CD4, CD8, and CD40-L expression, most of the CD40-L expression was associated with CD4+8- (~60%) and CD4+8+ (~25%) thymocyte populations (Figure 2b). Within the CD4+8+ thymocyte subset, CD40-L was preferentially expressed by cells expressing higher levels of CD4 and CD8 (Figure 2b).



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Figure 2. Flow cytometric analyses of CD40-L expression by thymocytes. (a,b) Data obtained from thymocytes cultured for 1 hr in medium before analysis. (c,d) Data obtained from thymocytes cultured for 1 hr in medium containing PMA and ionophore before analysis. (a,c) Histograms of CD40-L expression by unfractionated thymocytes. Open profile, MR-1; hatched profile, control. (b,d) CD40-L, CD8, and CD4 labeling conducted in FL1, FL2, and FL3, respectively. Left panels in b and d depict CD4 and CD8 expression of all thymocytes in control and experimental groups, respectively; right panels show the CD4/CD8 phenotype of MR-1+ thymocytes.

Exposure of thymocytes to PMA and ionophore for 1 hr resulted in a rapid upregulation of CD40-L (Figure 2c). The distribution of CD40-L+ cells among thymocyte populations defined by CD4/CD8 expression was similar to that of untreated thymocytes, although there was further skewing of MR1+ cells to the CD4+8- population (Figure 2d). Although this treatment had little effect on the representation of thymocyte subsets defined by CD4 and CD8 expression, marked downregulation of CD4 labeling was observed after PMA/ionophore treatment.

Immunohistochemical analysis of gp 39 expression in frozen sections of adult thymus tissue detected rare, scattered CD40-L+ cells in the cortex and more labeling in areas corresponding to the medulla (Figure 3a). A punctate anti-CD40-L labeling pattern predominated among medullary thymocytes, although profiles of partial circumferential labeling were also observed (Figure 3b). Demonstration of CD3 expression on adjacent thymic sections (Figure 3c) confirmed that the location of the majority of the CD40-L+ thymocytes coincided with the distribution of strongly labeled CD3+ cells in the medulla.



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Figure 3. Thymic expression of CD40-L. (a) CD40-L+ thymocytes are predominantly localized to the medullary environment. Dots outline capsule. (b) Higher magnification of anti-CD40-L labeling to demonstrate the patchy and granular nature of the staining pattern. Often reaction product is restricted to small spots (arrowheads). (c) Adjacent serial section processed to demonstrate CD3 expression. Endogenous peroxidase activity not blocked. Bars: a,c = 100 µm; b = 20 µm.

Ontogeny of Thymic CD40 and CD40-L Expression
At Day 14 of gestation, the earliest time point examined, expression of CD40 was preferentially associated with aggregates of cells in the central portion of the thymus (Figure 4a). With advancing gestational age (Days 16 and 18), increased levels of CD40 labeling were associated with cords of medullary cells (Figure 4b and Figure 4c, respectively). Levels of cortical CD40 expression observed in adult thymic tissue were not observed in fetal thymic tissue through Day 18 of gestation, but were dramatically increased by birth (Figure 4d). As shown in Figure 4e and Figure 4g, CD40-L+ thymocytes were not detectable in Day 18 fetal thymic tissue. By birth (Figure 4f and Figure 4h), CD40-L+ thymocytes were readily detectable and were preferentially associated with the medullary compartment, although isolated CD40-L+ thymocytes were scattered throughout the cortex. The upregulation of cortical CD40 expression and the appearance of CD40-L+ thymocytes coincided temporally with consolidation of the medullary thymic compartment. Scattered foci of ER-TR5+ and CD40+ epithelial cells present at Day 18 of gestation (Figure 4i) had coalesced into fewer but larger foci by birth (Figure 4j). The distribution of ER-TR5+ and medullary CD40+ cells was largely overlapping, suggesting that many of the ER-TR5+ stromal cells also expressed CD40 at this stage of development.



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Figure 4. Ontogeny of thymic CD40 and CD40-L expression. CD40 expression at fetal Days 14 (a), 16 (b), 18 (c) and at birth (d). CD40-L expression at fetal Day 18 (e,g) and birth (f,h). C, cortex; M, medulla. (i,j) Distribution of epithelial cells reacting with the ER-TR5 MAb in the Day 18 fetal and neonatal thymus, respectively. Endogenous peroxidase activity not blocked. Bars: a-f,i,j = 100 µm; g,h = 0 µm.

Targeted Overexpression of CD40-L to the Thymus Results in Altered Cortical, Medullary, and Subcapsular Compartments
The temporal coincidence of the appearance of CD40-L+ thymocytes and the organization of the medulla raised the possibility that CD40-L/CD40 interactions might contribute to the differentiation or expansion of this tissue compartment. Therefore, we examined the organization and phenotype of thymic tissue in which the thymocyte expression of CD40-L was abrogated (Xu et al. 1994 ) or elevated through targeted transgene expression.

Thymic tissue from mice bearing a disruption of the CD40-L gene displayed fairly normal histology, with well-defined cortical and medullary compartments and no obvious defect in thymocyte development (Xu et al. 1994 ), although some aspects of the selection process appear to be altered (Foy et al. 1995 ). Immunohistochemical analyses of the thymic environment of CD40-L-deficient mice detected subtle decreases in medullary expression of B7-2 (Foy et al. 1995 ) CD40, B7-1, and Class II MHC molecules (data not shown), but clearly indicated that CD40-L/CD40 interactions did not play an obligate role in establishment of cortical or medullary thymic compartments.

Thymic tissue from transgenic mice expressing different levels of CD40-L transgene under control of the proximal lck promoter were examined to assess the consequences of CD40-L overexpression on the organization and development of thymic stromal compartments. The extent to which overexpression of CD40-L affected the thymic environment was correlated with the number of CD40-L transgene copies, which in turn correlated with levels of transgene expression. In thymic tissue from transgenic mice expressing 40 copies of the transgene (Figure 5), the thymic epithelial component identified by E-cadherin expression (Lee et al. 1994 ) was dramatically reduced and did not extend to the capsule, which resulted in a variable expansion of the extraepithelial subcapsular space (Figure 5d and Figure 5g). In some regions of the thymus from high copy-number mice, scattered globular ER-TR4+ cells were distributed throughout areas that labeled extensively with ER-TR5 MAb, a medulla-restricted reagent, in adjacent serial sections. The scattered ER-TR4+ cells in the transgenic thymus resembled the occasional medullary ER-TR4+ cells observed in normal thymic tissue (compare insets of Figure 5b and Figure 5e). In other thymic tissue from this line of mice, cortical ER-TR4+ cells were reduced in frequency but retained reticular profiles, and were distributed in a pattern congruent with medullary ER-TR5+ cells (Figure 5h and Figure 5i). These two patterns of stromal cell disorganization could be found within the same thymus. Similar results were obtained with 6C3 and G8.8 MAbs, which preferentially label cortical and medullary epithelial cells, respectively (data not shown).



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Figure 5. Thymic alterations due to targeted overexpression of CD40-L in thymocytes. Normal thymic tissue (a-c) or transgenic thymic tissue (d-i) was processed to demonstrate all thymic epithelium with anti-E cadherin MAb (a,d,g), cortical epithelium with ER-TR4 MAb (b,e,h), and medullary epithelium with ER-TR5 MAb (c,f,i). In b, medullary ER-TR4+ cells are indicated by arrowheads. Capsule is indicated by asterisk. Endogenous peroxidase has been blocked. Bars = 100 µm; insets = 10 µm.

In addition to the disrupted segregation/representation of cortical and medullary epithelial cells, the expression patterns of several putative cell interaction molecules were elevated in the lckCD40-L transgenic thymus tissue. Labeling of B7-1 and B7-2 was widespread throughout the epithelial and extraepithelial thymic compartments (data not shown). Dendritic cells and macrophages defined by CD11c expression were also widely distributed throughout both thymic compartments (data not shown).

The expanded extraepithelial subcapsular space defined by the absence of E-cadherin+ epithelium (Figure 6a) contained accumulations of B220+ (Figure 6b) and CD40+ (Figure 6c) cells. Widespread CD40 expression was also evident within the epithelial compartment of the thymus (Figure 6c). The extraepithelial compartment also contained substantial numbers of CD3+ (Figure 6d) cells expressing CD40-L (Figure 6e). Although the level of CD40-L expression was dramatically elevated in the transgenic thymic tissue, the punctate labeling pattern was similar to that observed in normal mice (inset to Figure 6e). In addition to B-cells and CD3+ cells, these areas also contained significant numbers of CD11c+ cells (Figure 6f) and thus resembled diffuse peripheral lymphoid tissue. Endogenous peroxidase activity was predominantly associated with the epithelial component of the transgenic thymic tissue (Figure 6g). As in normal thymic tissue, this activity was due largely to eosinophils, as determined by ultrastructural cytochemistry (data not shown).



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Figure 6. Thymic capsular connective tissue acquires characteristics of secondary lymphoid tissue in CD40-L transgenic mice. Adjacent sections of transgenic thymus tissue were processed to demonstrate E-cadherin (a), B220 (b), CD40 (c), CD3 (d), CD40-L (e), and CD11c (f). Omission of primary MAb revealed background endogenous peroxidase activity (g). Arrow indicates edge of capsule. Endogenous peroxidase activity has not been blocked. Note that endogenous peroxidase activity is largely restricted to the epithelial compartment of the thymus. Arrowheads in inset of e indicate the punctate nature of CD40-L expression. Inset is enlargement of extracapsular region devoid of endogenous peroxidase activity. Bars: a-g = 100 µm; inset = 10 µm.

Elevated intrathymic CD40-L expression could be affecting the initial expansion of different stromal cell populations or the differentiation program of TE subpopulations. If promiscuous CD40-L/CD40 interactions were primarily altering the expansion potential of different TE populations, thymocytes overexpressing CD40-L would not be expected to significantly alter the representation of relatively radio-resistant cortical and medullary elements in an established thymic environment that had developed normally. On the other hand, if the loss of cortical TE and expansion of the medullary compartment within the lckCD40-L thymus reflected disruption of the normal developmental relationship between these two epithelial populations, then elevated levels of CD40-L expression should cause similar epithelial alterations in thymic tissue that had developed normally. We generated radiation bone marrow chimeric mice by lethally irradiating C57Bl/6 mice and reconstituting their hematopoietic compartment with T-cell-depleted bone marrow from syngenic normal mice or from lckCD40-L mice. As shown in Figure 7, reconstitution of irradiated but otherwise normal adult thymic tissue with syngenic bone marrow cells resulted in normal thymic architecture. In contrast, repopulation of normal thymic tissue with thymocytes overexpressing CD40-L resulted in a thymic phenotype virtually indistinguishable from that displayed by lckCD40-L transgenic mice. In addition to loss of cortical TE and expansion of medullary TE, lckCD40-L &Aelig; C57Bl/6 thymic tissue also displayed accumulations of N418+ cells, elevated levels of CD40 expression, and focal subcapsular accumulations of T-cells, B-cells, and dendritic cells.



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Figure 7. Reconstitution of normal irradiated mice with bone marrow from transgenic mice results in an altered thymic phenotype. C57Bl/6 mice were lethally irradiated and then reconstituted with T-cell-depleted bone marrow from syngeneic (a-d) or lckCD40-L (e-h) mice. Sections were labeled with MAbs specific for cortical epithelium (ER-TR4; a,e), medullary epithelium (ER-TR5; b,f), anti-CD40 MAb (c,g), or anti-CD11c (N418; d,h). Asterisk indicates region of subcapsular accumulation of lymphocytes and arrowheads indicate cortical epithelial processes labeled with ER-TR4 MAb. Bars = 100 µm.


  Discussion
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Materials and Methods
Results
Discussion
Literature Cited

The pattern of CD40 expression in the murine thymus was similar to that described for the human thymus (Galy and Spits 1992 ) and resembled the distribution of Class II MHC antigens within the adult thymus (Farr and Nakane 1983 ; Rouse et al. 1979 ). Whereas cortical CD40 was predominantly associated with epithelial cells, cells expressing CD40 in the medulla were heterogeneous and included dendritic cells and thymic epithelial cells. The basis for the variable expression of CD40 by medullary epithelial cells is presently not clear and may represent additional epithelial heterogeneity or local microenvironmental influences on the levels of CD40 expression.

Flow cytometric and immunohistochemical analyses indicated that CD40-L is expressed at low levels by thymocytes, but preferentially by medullary CD4+8- and, to a lesser extent, CD4+8+ subsets. The distinctive punctate nature of much of the MR1 labeling may represent CD40-L sequestered in a cytoplasmic compartment within thymocytes or redistribution of CD40-L on the thymocyte cell surface as a consequence of crosslinking induced by interactions with CD40+ cells in the thymus. Dynamic regulation of CD40-L expression by CD4+ thymocytes is suggested by reports that CD40-L/CD40 interactions lead to downregulation of CD40-L expression by CD4+ T-cells (Yellin et al., 1992), and that human CD4+ memory T-cells contain preformed CD40-L that is rapidly brought to the cell surface after TCR-mediated activation (Casamayor-Palleja et al. 1995 ). The increased levels of CD40-L expression observed 1 hr after PMA/ionophore stimulation would be consistent with the possibility that CD40-L can be mobilized from intracellular sites.

Previous studies of SCID or RAG-deficient mice demonstrated that normal development and organization of medullary, but not cortical, thymic epithelium was dependent on the participation of mature CD3+ thymocytes (Shores et al. 1991 , Shores et al. 1994 ; Surh et al. 1992 ). In normal mice this is reflected by conversion of scattered medullary foci present in the fetal thymus to a more consolidated adult pattern at about birth, when mature single positive thymocytes expressing {alpha}ß TCR begin to accumulate (Havran and Allison 1988 ). The augmented expression of cortical CD40 and the appearance of predominantly medullary CD40-L+ thymocytes during late fetal development occurred simultaneously with reorganization of the medullary epithelial compartment, and may reflect an interdependence of these processes. Expression of CD40 by murine thymic stromal cells can be upregulated in vitro by exposure to TNF{alpha} or combinations of IFN{gamma} and other pro-in flammatory cytokines (Farr, unpublished observations). If the cytokine requirements for CD40 expression by TE cells in vitro reflect physiological requirements for this process in vivo, the neonatal upregulation of CD40 expression may be related to the accumulation of CD4 and CD8 single positive thymocytes, which can produce high titers of IFN{gamma} and TNF{alpha} in vitro after activation with calcium ionophore and PMA (Fischer et al. 1991 ) and which may be releasing these cytokines in response to physiological stimuli in situ.

Foy et al. 1995 have reported that negative selection in some model systems, but not in others, is disrupted in CD40-L-deficient mice and in mice chronically treated with anti-CD40-L MAb. They also documented a reduction of B7-2 expression within the medullary compartment of CD40-L-deficient mice and suggested that CD40-L/CD40 interactions may exert their effects in part by modulating expression of other cell interaction molecules within the thymic environment. Our demonstration that medullary, but not cortical, levels of other potential cell interaction molecules, including Class II MHC antigens, CD40, and B7-1, are reduced in gp 39-deficient mice lends additional support to this hypothesis. Lower levels of these cell interaction molecules within the thymic medulla, particularly MHC molecules, might decrease the efficiency of negative selection of some self-reactive thymocytes without having a significant impact on the overall production of thymocytes.

Targeted overexpression of CD40-L in thymocytes resulted in reduced thymic cellularity, disrupted thymocyte development, and profound alterations in the phenotype and organization of the thymic environment, the severity of which increased with increased transgene expression. At low levels of transgene expression (<12 copies) thymic cellularity was reduced two- to 10-fold, with fairly normal representation of thymocyte subsets defined by CD4 and CD8 expression. With high copy number there was a preferential loss of CD4+8+ thymocytes (up to 4000-fold reduction) and skewing of the mature thymocyte representation to the CD8 single positive subset. Thymocyte development in radiation bone marrow chimeras repopulated with transgenic bone marrow yielded similar results (Clegg CH et al., submitted for publication). Based on the work of Foy et al. 1995 demonstrating that CD40/CD40-L interactions participate in negative selection, it is tempting to speculate that this process is perturbed in the lckgp39 transgenic mice.

A potential role for CD40-L/CD40 interactions in regulating epithelial cell growth/differentiation is suggested by the progressively increased representation of medullary TE cells and the decline in the frequency of cortical TE cells as levels of transgene-derived CD40-L expression increased. It is presently not known if cortical and medullary epithelia derive from a common 3rd pharyngeal pouch endodermal epithelial progenitor population (Le Douarin et al. 1984 ) or reflect expansion of separate epithelial cell populations derived from 3rd pharyngeal pouch endoderm and ectoderm from the 3rd pharyngeal cleft (Cordier and Haumont 1980 ). Studies of thymic epithelial cell differentiation in vitro have described terminal differentiation of medullary epithelial cells (Lobach et al. 1987 ), but acquisition of a medullary phenotype by cortical epithelial cells has not been demonstrated in vitro. The congruent localization of cortical and medullary epithelial cells in both low and high copy-number mice, and the replacement of cortical with medullary epithelium in some areas of thymic tissue from high copy-number mice, may result from changes in the developmental program of thymic epithelium, resulting in the differentiation of cortical epithelium to express a medullary phenotype or from alterations within the thymic environment that favor the growth/maintenance of a discrete medullary epithelial population. The ability of T-cell-depleted lckCD40-L bone marrow cells to reproduce the transgenic thymic phenotype in normal lethally irradiated recipients suggests that the cortical and medullary epithelial populations may represent different facets of a common differentiation pathway. The similar globular profiles of many of the ER-TR4+ cells in the transgenic thymus and scattered ER-TR4+ medullary cells in the normal thymus raise the possibility that the processes affecting cortical TE in the transgenic thymus may also be occurring in the normal thymus, but on a much smaller scale. Alternatively, the loss of cortical TE observed in the lckgp39 thymus may reflect selective elimination of cortical TE as a consequence of inappropriate CD40/CD40-L interactions. Apoptosis of human CD40+ epithelial cell lines as a consequence of CD40 ligation has been reported (Hess and Engelmann 1996 ).

The observed alterations in the thymic environment may not represent a direct sequelae of CD40/CD40-L interactions. Alterations of thymocyte development as a consequence of dysregulated expression of CD40L could modify other stimuli that in turn regulate the differentiation of thymic epithelial cells. A loss of cortical epithelium has been reported in transgenic mice that are homozygous for a human CD3{epsilon} transgene and display a block in thymocyte differentiation at the CD44-CD25- stage (Hollander et al. 1995 ). However, in contrast to the CD40-L transgenic thymus, the lack of cortical epithelial development in the CD3{epsilon} transgenic mice does not appear to be accompanied by expansion of the medullary compartment or capsular involvement, suggesting that the requirements for expansion and maintenance of cortical TE may be different. Interestingly, thymic tissue from transgenic mice with thymic overexpression of oncostatin M (Clegg and Farr, manuscript in preparation) or LIF (Shen et al. 1994 ) exhibits alterations that resemble some aspects of the lckCD40-L thymic phenotype, such as expansion of the medullary compartment and mixed cellular infiltrates in the capsule. The ability of CD40 ligation to stimulate GM-CSF production by human thymic epithelium in vitro (Galy and Spits 1992 ) raises the possibility that altered cytokine production by thymic epithelial cells in response to elevated CD40-L/CD40 interactions may contribute to these alterations. Future studies directed at the effects of CD40 engagement on the functional /phenotypic characteristics of CD40+ thymic epithelial cell lines may provide mechanistic insights into the influence of CD40-L/CD40 interactions on the thymic environment.


  Acknowledgments

This work was supported by grants from the National Institutes of Health (AI-24137 and AG-04360) and by the Bristol-Myers Squibb Pharmaceutical Research Institute.

We thank Dr Roger Perlmutter for critical review of the manuscript.

Received for publication May 15, 1996; accepted August 5, 1996.


  Literature Cited
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Summary
Introduction
Materials and Methods
Results
Discussion
Literature Cited

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