1 Shichinohe Research Unit, National Institute of Animal Health, Shichinohe, Aomori 039-2586, Japan
2 Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691-4096, USA
Correspondence
Hiroshi Tsunemitsu
tsunemi{at}affrc.go.jp
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ABSTRACT |
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The GenBank/EMBL/DDBJ accession number for the sequence of the VP6 gene of bovine group B rotavirus Nemuro strain is AB106542.
Present address: Department of Infectious Diseases, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
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INTRODUCTION |
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Group B rotaviruses (GBRs) cause diarrhoea in humans, pigs, cattle, lambs and rats (Bridger, 1994; Bridger et al., 1982
; Mackow, 2002
; Mebus et al., 1978
; Saif, 1990
; Theil et al., 1985
). In humans, GBRs have been associated with large outbreaks of gastroenteritis, mainly in adults in China; however, outside China, there have been only a few reports of GBR detection (Ahmed et al., 2004
; Hung et al., 1984
; Kobayashi et al., 2001
; Krishnan et al., 1999
; Mackow, 2002
). In pigs, GBRs have been detected in nursing- and weanling-pig diarrhoea (Bridger, 1994
; Janke et al., 1990
; Saif, 1990
) and GBR infection in cattle has been reported in the USA, UK, India and Japan (Chang et al., 1997
; Khurana & Pandey, 2001
; Saif et al., 1991
; Snodgrass et al., 1984
; Tsunemitsu et al., 1999
). Outbreaks of diarrhoea in dairy cows caused by bovine GBR have been observed in Japan (Hayashi et al., 2001
; Tsunemitsu et al., 1999
). However, reasons for the prevalence of GBR infection in cattle remain unclear because, with the exception of one porcine strain (Sanekata et al., 1996
), serial propagation of GBRs in cell culture has been unsuccessful and immunological reagents for detecting GBR antigens and antibodies are available in only a few laboratories.
The major capsid protein of rotaviruses, VP6, is highly antigenic and immunogenic. VP6 is a major component of the virion, accounting for approximately 51 % by weight of the virion in GARs (Mattion et al., 1994). Group-antigen epitopes are detected predominantly on VP6 in each rotavirus group (Kapikian et al., 2001
; Mattion et al., 1994
). In GARs, group antigens generally cause extensive cross-reactivity between strains in serological tests, e.g. immunofluorescence (IF) and enzyme-linked immunosorbent assays (ELISA). Therefore, several diagnostic assays directed against these antigens have been developed for GAR infection and are available for use with clinical samples from many species, at least among mammals. In contrast, most avian GARs react with only a limited set of broadly cross-reacting mammalian VP6-specific mAbs (Brüssow et al., 1992
), suggesting the absence of certain epitopes of group antigens in avian GARs (Saif et al., 1994
). These antigenic relationships correspond to the similarity in VP6 polypeptide sequences, with >87 % amino acid identity within mammalian GARs and 7075 % amino acid identity between mammalian and avian GARs (Ito et al., 1995
; Tang et al., 1997
).
Information about the extent of VP6 variation in GBRs is limited. To date, GBR VP6 gene sequences have been determined only for human and rodent species (Chen et al., 1991; Eiden et al., 1992
; Mackow, 2002
). The extent of VP6 variation between human and rodent strains was greater than that reported for heterologous mammalian GARs, although recombinant VP6 from the rodent strain IDIR reacted with antisera against homologous and heterologous GBRs, including the human strain ADRV (Lindsay et al., 1993
). On the basis of nucleic acid hybridization, Eiden & Allen (1992)
indicated that the VP6 gene of the IDIR strain might be related more closely to that of the ADRV strain than to that of bovine GBR. There have been no further reports, however, on the genetic characterization of the VP6 gene of bovine GBR.
In this report, we determined the complete sequence of the VP6 gene of the bovine GBR Nemuro strain, derived from an adult cow with diarrhoea in Japan. To our knowledge, this is the first report on the GBR VP6 gene sequence from a domesticated animal. Sequence comparison with the cognate genes of human and rodent GBRs showed a remarkable diversity among GBRs, suggesting that, compared with GARs and group C rotaviruses (GCRs), GBRs derived from different mammalian species are more genetically divergent. We also generated recombinant VP6 protein with a baculovirus expression system and developed an ELISA using the expressed protein for the detection of GBR antibodies in cattle and pig sera. Our seroepidemiological study indicated the common occurrence of GBR infection in cattle and pigs and the necessity of clarifying its aetiological role in diarrhoea in these animals.
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METHODS |
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Serum specimens.
In total, 520 serum samples obtained from cattle and pigs were tested for antibodies for GBR. The 380 bovine serum samples included (i) 13 paired sera from adult cows with field GBR infections from two outbreaks in Japan (Hayashi et al., 2001; Tsunemitsu et al., 1999
), (ii) four convalescent-phase sera from colostrum-deprived calves that were inoculated experimentally with bovine GBR Nemuro or GAR 22R (Matsumura et al., 2002
), (iii) eight pre-exposure sera collected from colostrum-deprived calves and (iv) 342 sera from 33 farms of dairy cattle in Japan, aged between 4 months and 11 years. The 140 porcine serum samples included (i) 10 pre-exposure sera from gnotobiotic pigs, (ii) 30 sera from a farm of pigs grouped by age from 1 to 6 months and (iii) 100 sera from nine farms of sows in Japan.
Cloning and sequence analysis.
Synthetic primer 1 (5'-CCCGTCGACGAATTCTTT-3'-NH2) was ligated to the 3' ends of the viral RNA as described by Lambden et al. (1992). cDNAs were produced by RT-PCR using primer 2 complementary to primer 1 and an oligonucleotide primer correponding to either the plus sense of the 5' end or the minus sense of the 3' end of the ADRV GBR VP6 gene (Chen et al., 1991
). The cDNAs were cloned into the pGEM-T vector (Promega) and sequenced by cycle sequencing with an automatic DNA sequencer (ABI PRISM 310; Applied Biosystems). Confirmation of both terminal sequences was also provided by use of a 5' rapid amplification of cDNA ends system (5' RACE; Invitrogen). The full-length VP6 gene cDNA was produced by RT-PCR with 5'- and 3'-end primers designed with the determined terminal sequences, and the PCR product was also sequenced directly by cycle sequencing.
Construction of baculovirus recombinant.
The full-length Nemuro VP6 gene cDNA was produced by RT-PCR with the oligonucleotides 5'-ACGAATTCAATTAGTCCAATCAGGGTG-3' [primer sequence with a flanking EcoRI site (underlined) and the 5' end of the VP6 gene of the Nemuro GBR] and the 5'-ATGGATCCTCCTCTAGAGCAACCCGGTTC-3' [primer sequence with a flanking BamHI site (underlined) and the complement of the 3' end of the VP6 gene of the Nemuro GBR], digested with EcoRI and BamHI and cloned into the EcoRIBamHI sites of the pVL1392 vector (BD PharMingen). Cotransfection of Spodoptera frugiperda (Sf9) cells with the recombinant pVL1392 and the Baculogold linearized baculovirus DNA (BD PharMingen) was performed by using Lipofectin (Invitrogen) in accordance with the manufacturer's instructions. The expression of GBR antigens in Sf9 cells infected with the baculovirus recombinant was identified by indirect IF (Tsunemitsu et al., 1999) and Western blot assays.
SDS-PAGE and Western blot assay.
Sf9 cells were infected with the baculovirus recombinant or wild-type (wt) Autographa californica multiple nuclear polyhedrosis virus (AcNPV) at an m.o.i. of 10 p.f.u. per cell and incubated for 3 days. Harvested cells were washed twice with PBS (pH 7·4). Cells were dissolved in SDS-PAGE sample buffer [12 mM Tris/HCl (pH 6·8), 5 % glycerol, 0·4 % SDS, 10 % -mercaptoethanol and 0·02 % bromophenol blue] and boiled for 10 min. Proteins were dissociated by SDS-PAGE analysis with 4 % stacking and 10 % separating gels. Proteins on the gel were stained with Coomassie blue or electroblotted onto a PVDF membrane (Bio-Rad). The membrane was blocked with 5 % BLOTTO (5 % non-fat dry milk and 0·1 % Tween 20 in PBS) and incubated with bovine anti-GBR serum diluted 1 : 4000. Bound antibodies were detected by incubation with horseradish peroxidase-conjugated anti-bovine IgG (Kirkegaard & Perry Laboratories) and ECL reagents (Amersham Biosciences).
ELISA for detection of GBR antibodies.
ELISA plates (MaxiSorp; Nalge Nunc International) were coated with lysates of Sf9 cells infected with either the baculovirus recombinant (antigen-coated wells) or wt AcNPV (non-antigen-coated wells). Antigen was prepared from cells that had been lysed with 50 mM Tris/HCl buffer (pH 8·0) containing 150 mM NaCl, 1 % NP40, 0·5 µg aprotinin ml1 and 0·5 µg leupeptin ml1 and clarified by centrifugation at 10 000 g for 20 min. Each well received 100 µl lysate containing 0·4 µg protein as measured by the DC protein assay (Bio-Rad). Plates were stored at 4 °C overnight and washed with PBS containing 0·05 % Tween 20 (PBST). Next, plates were blocked with 5 % BLOTTO at 37 °C for 1 h and washed with PBST. Bovine or porcine test sera were diluted 1 : 100 or serially from 1 : 100 to 1 : 25 600 with PBST/1 % non-fat dry milk. Plates were incubated with diluted test sera at 4 °C overnight and washed five times with PBST. Next, peroxidase-conjugated anti-bovine or anti-porcine IgG (Kirkegaard & Perry Laboratories) was added, incubated at 37 °C for 1 h and washed five times with PBST. Finally, ABTS [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)] substrate was added and A405 was recorded. The GBR antibody data were expressed as net A405, which was calculated as A405 from antigen-coated wells minus A405 from non-antigen-coated wells. Positive reactions were identified by the net A405 of samples >7SD above the mean value for sera from pre-exposure colostrum-deprived calves or from pre-exposure gnotobiotic pigs.
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RESULTS |
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Comparison of the VP6 gene of the Nemuro strain with those from the human ADRV, CAL-1, Bang373 and WH-1 strains and the rodent IDIR strain indicated identities of 6667 % in nucleotide and 7072 % in deduced amino acid sequences, which were lower than those between human and rodent viruses (7273 and 8384 %, respectively) (Table 1). The overall identities of VP6 genes among GBRs were substantially lower than those among GARs and GCRs derived from different species of mammals (Table 1
). Multiple alignments of the amino acid sequences of VP6 produced by using the MegAlign program (DNASTAR) indicated that the N-terminal regions of the VP6 were relatively conserved among GBRs; in contrast, however, no conserved regions were observed between GBR and GAR or GCR strains. Five of six cysteine residues in the Nemuro GBR occurred at positions identical to those in other GBR strains (aa 10, 194, 213, 278 and 337). Two (aa 194 and 337) of these were located almost identically in GAR (aa 197 and 331) and GCR (aa 188 and 336).
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Four convalescent-phase sera from newborn calves infected experimentally with bovine GBR (two calves) or GAR (two calves) and paired sera from 13 adult cows infected naturally with bovine GBR were used to evaluate the ELISA. Convalescent-phase calf sera directed against bovine GBR were positive for GBR antibodies until the serum was diluted 1 : 4001 : 1600. In contrast, convalescent-phase calf sera against bovine GAR were negative in the ELISA (Fig. 2a). All paired sera from adult cows diluted 1 : 100 demonstrated increases in GBR antibodies in the ELISA (Fig. 2b
).
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DISCUSSION |
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The large divergence of GBR VP6 sequences from different species suggests that the GBRs of different species may have had a longer period of time to diverge from one another than have GARs and GCRs. Furthermore, VP6 sequence diversity might reflect previous observations of antigenic differences between GBRs in reactivity on immunoelectron microscopy and ELISA (Eiden et al., 1986; Nakata et al., 1986
), despite of the presence of group antigens on VP6 (Eiden et al., 1994
; Yolken et al., 1988
). VP6 among GBRs from diferent species may have fewer epitopes that define group antigenicity than those of GARs and GCRs.
Comparisons of VP6 sequences between different serogroups confirmed that GAR and GCR VP6 are related more closely to each other than to GBR VP6. Antigenic cross-reactivity has never been observed between GBRs and GARs or GCRs (Saif, 1990), whereas we previously reported the presence of cross-reactive epitopes on VP6 between GARs and GCRs (Tsunemitsu et al., 1992
).
Disulfide bonds have been implicated in the intramolecular tertiary structure of the GAR and GCR VP6 (Estes et al., 1987; Sabara et al., 1987
; Tosser et al., 1992
). All GBR VP6 proteins contain six cysteine residues, double the number found in GAR and GCR VP6 proteins (Mattion et al., 1994
), and five cysteine residues are conserved within the GBR VP6. Two of these are located almost identically in GAR and GCR VP6, suggesting that these cysteine residues are likely to be involved in the tertiary forms of rotavirus VP6 functional domains.
The production of large amounts of recombinant Nemuro VP6 expressed in the infected Sf9 cells facilitated the development of an ELISA for GBR-antibody detection. The antibody ELISA in the present study was specific for GBRs and showed no reaction to convalescent-phase sera of calves infected experimentally with GAR. Further, the sensitivity of the assay is suitable for the investigation of bovine GBR infection because all paired sera from cows infected naturally with GBR showed an increase in GBR-antibody titres. In the present study, we were not able to examine the reactivity of the recombinant bovine VP6 with antisera against human and rodent GBRs.
There have been few reports of the antibody prevalence of GBR in cattle. No GBR antibody was found in cattle sera in China (Hung et al., 1985), whereas 2071 % of cattle sera were positive for GBR antibodies in the UK (Bridger, 1987
; Brown et al., 1987
). The antibody prevalence in the present study was similar to that in the UK. The positive rate of GBR antibodies in sera from young cattle was lower than that in adult cattle. This result suggests that GBR infection in cattle is common in Japan, especially in adult cattle, supporting our recent reports on outbreaks of diarrhoea in adult cows with GBR infection (Hayashi et al., 2001
; Tsunemitsu et al., 1999
). Further, the difference in antibody prevalence among age groups in the present study may indicate that the risk of GBR exposure in cattle is age-related. Interestingly, similar findings have been reported for GBR and GCR infections in humans (Hung et al., 1984
; Oishi et al., 1993
; Steele & James, 1999
). Further studies are needed to compare the sensitivity to, and pathogenicity of, bovine GBR between young and adult cattle.
We could not verify the sensitivity of the present ELISA against porcine GBR infection. However, this assay showed a very high prevalence of GBR antibodies in sera from adult pigs, suggesting that it may also be suitable for the investigation of porcine GBR infection. The prevalence of GBR antibodies in pigs varies among reports, with infection spread widely in the UK and Australia (antibody prevalence of 5997 %), but apparently less widely in China and the USA (2336 %) (Bridger & Brown, 1985; Brown et al., 1987
; Hung et al., 1985
; Nagesha et al., 1988
; Theil & Saif, 1985
). The very high prevalence of GBR antibodies reported here suggests that GBR infection in pigs may be ubiquitous in Japan. Further, the present study suggests that GBR infection in pigs may largely occur by 23 months of age (probably in post-weaning pigs). In contrast, however, only one report has described the detection of porcine GBR in Japan (Sanekata et al., 1996
). The reason for this apparent discrepancy might relate more to the fact that GBRs are generally not investigated (owing to the difficulty of GBR detection), rather than to any asymptomatic status of most GBR infections in pigs. One report indicated that GBR was frequently implicated in diarrhoea in both nursing and post-weaning pigs (Janke et al., 1990
). Further studies to define the aetiological role of GBR infection in pig and cattle diarrhoea are in progress.
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Received 31 January 2005;
accepted 15 June 2005.
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