1 Animal Diseases Research Unit, USDA-Agricultural Research Service, Washington State University, PO Box 646630, Pullman, WA 99164-6630, USA
2 Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
3 US Sheep Experiment Station, USDA-Agricultural Research Service, Dubois, ID, USA
Correspondence
Naomi S. Taus
tausns{at}vetmed.wsu.edu
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ABSTRACT |
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Present address: Grays Harbor Veterinary Services, 4 Old Beacon Road, Montesano, WA 98563, USA.
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MAIN TEXT |
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OvHV-2 has not been propagated in vitro, although lymphoblastoid cell lines infected with OvHV-2 have been derived from cattle, deer and rabbits with clinical disease (Buxton et al., 1984; Reid et al., 1989
). Cell lines derived from red deer and rabbits transmitted disease to deer and rabbits (Reid et al., 1983
, 1989
); however, cell lines derived from cattle did not induce disease (Reid et al., 1989
). Injection of blood and tissues from clinically affected animals (deer and cattle) into calves, deer, bison and rabbits has been used to transmit MCF; however, the efficiency of disease transmission was highly variable (Abu Elzein et al., 2003
; Buxton & Reid, 1980
; Clark et al., 1972
; Huck et al., 1961
; Liggitt et al., 1980
; Reid et al., 1983
, 1986
).
Studies of OvHV-2 infection in sheep have had to rely on natural transmission from infected sheep to uninfected sheep introduced into an infected flock (Hussy et al., 2001, 2002
; Li et al., 1998
, 2000
). Experimental infection of sheep, using whole blood from infected sheep, has been achieved, but large volumes (100 ml) of blood were needed and only two of five inoculated animals became infected (Li et al., 2000
).
In this study, four experiments were conducted to characterize the infectivity of nasal secretions collected from OvHV-2-infected sheep experiencing intense viral shedding episodes. In experiment 1, nasal secretions were collected daily as described previously (Kim et al., 2003) from 16 56-month-old OvHV-2-infected cross-bred lambs obtained from the US Sheep Experiment Station (USSES), Dubois, ID. Total DNA, consisting of viral DNA and variable amounts of DNA from host cells and bacteria, was extracted from the secretions as described previously (Li et al., 2004
). Real-time PCR, adapted from a previous report (Hussy et al., 2001
; Li et al., 2004
), was used to quantitate the viral genome copy numbers. One sheep whose nasal secretions contained
100 000 copies of viral DNA per 2 µg total DNA was identified and further secretions were collected 56 h after the initial sampling. The secretions were diluted in 12 ml PBS, pH 7·4, and clarified at 228 g for 10 min. Because virus shedding in nasal secretions can diminish dramatically within 24 h (Li et al., 2004
), the genome copy number in the clarified secretions was requantified.
Two 9-month-old sheep, obtained from the OvHV-2 uninfected flock maintained in accordance with animal care and use protocols at Washington State University (WSU), Pullman WA, were nebulized, as described previously (Li et al., 2004), with 2 ml of the secretions containing approximately 3x107 viral genome copies. Aerosolization was selected as the route of administration because it represents a likely natural route of virus transmission (Li et al., 2000
, 2001a
; Mushi et al., 1980
). The nebulized sheep, housed together in an isolation facility at WSU, were sampled daily for 90 days post-infection (p.i.), then weekly until 134 days p.i. Using a semi-nested PCR specific for OvHV-2 (Li et al., 1995
, 2004
), viral DNA was first detected in peripheral blood leukocytes (PBL) at 8 and 9 days p.i. (Table 1
). Real-time PCR was used to quantitate the levels of OvHV-2 DNA in the PBL. Peak genome copy numbers reached 3000 copies per 2 µg total DNA by 15 and 21 days p.i. then declined to a few hundred copies or became undetectable by real-time PCR (Fig. 1
, bottom panel). A competitive inhibition ELISA (cELISA), which detects antibody directed against an epitope conserved among MCF viruses, was used to detect MCF virus-specific antibody as described previously (Li et al., 2001b
). Virus-specific antibody in the plasma of the sheep was first detected at 10 and 11 days p.i. (Table 1
, Fig. 1
, top panel). Both animals remained seropositive for the duration of the experiment (Fig. 1
, top panel).
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In experiment 2, six 10-month-old sheep, from the WSU OvHV-2 uninfected flock, were each nebulized with 2 ml of the pooled nasal secretions containing 108 (n=2), 106 (n=2) and 104 (n=2) copies of OvHV-2 DNA. Two control sheep were nebulized with nasal secretions prepared from OvHV-2-negative sheep. Each group of infected sheep and control sheep were housed separately in an isolation facility. The sheep were sampled daily for 40 days p.i., bi-weekly from 44 to 61 days p.i., then weekly from 68 to 118 days p.i.
Using semi-nested PCR, viral DNA was first detected in PBL at 7 days p.i. in sheep receiving inoculum containing 108 copies of OvHV-2 DNA (Table 1). Sheep nebulized with secretions containing 106 copies became positive forOvHV-2 DNA in PBL at 9 days p.i. (Table 1
). Viral DNA was first detected in the PBL of sheep exposed to inoculum containing 104 copies at 11 and 12 days p.i. (Table 1
). The control sheep remained negative for viral DNA throughout the duration of the study.
Virus-specific antibody was first detected in the two sheep receiving the highest dose of virus at 9 and 10 days p.i. (Table 1). Animals nebulized with secretions containing 106 and 104 copies developed detectable anti-viral antibody by 12 and 24 days p.i., and 17 and 29 days p.i., respectively (Table 1
). Detectable virus-specific antibody was maintained in all sheep throughout the remainder of the study. Control sheep did not develop MCF virus-specific antibody at any time during the experiment.
Using real-time PCR, peak viral DNA levels, ranging from 20 000 to 560 000 genome copies per 2 µg total DNA, were detected in PBL at 1924 days p.i. in the sheep exposed to inocula containing 108 and 106 copies (Fig. 2, top and middle panels). Peak viral DNA levels (32 000 and 29 000 copies per 2 µg total DNA) in PBL of the animals receiving inoculum containing 104 copies were detected at 29 and 32 days p.i. (Fig. 2
, bottom panel). OvHV-2 DNA levels in PBL gradually declined to a few hundred copies per 2 µg total DNA (Fig. 2
).
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In experiments 1 and 2, we examined nasal secretions for the presence of OvHV-2 DNA following nebulization. Using real-time PCR, viral DNA was undetectable to sporadically detectable at low levels (up to 675 copies per 2 µl sample) in nasal secretions of all animals, with the exception of two sheep (HL21, expt. 1 and HL37, expt. 2). At 4 and 6 days p.i., high levels (202 000 and 94 000 genome copies per 2 µl sample) of viral DNA were detected in nasal secretions of these two sheep. These spikes of viral DNA may reflect active virus replication. In another study (Li et al., 2005), three sheep nebulized with nasal secretions containing >109 copies of OvHV-2 DNA all developed transiently detectable high copy numbers of viral DNA in their nasal secretions.
We have previously shown that most lambs raised under standard husbandry conditions do not become infected with OvHV-2 until after 23 months of age (Li et al., 1998) and that maternally derived antibodies do not delay infection of lambs (Li et al., 2002
). Experiment 3 was performed to test whether there is an age barrier to infection with OvHV-2. Two 6-day-old lambs from the WSU OvHV-2-negative flock were nebulized with 2 ml nasal secretions, from the same pool used in experiment 2, containing 104 copies of viral DNA. Blood samples were collected weekly from 7 to 49 days p.i. Both lambs developed detectable viral DNA in PBL by 14 days p.i. and detectable virus-specific antibody by 28 days p.i. (Table 1
). Therefore, age is not a barrier to infection with OvHV-2 in lambs.
Experiment 4 was conducted to determine the minimum sheep infectious dose of the pooled nasal secretions. Ten 23-month-old lambs from the WSU flock were nebulized with nasal secretions containing 104 (n=1), 103 (n=3), 102 (n=3) and 101 (n=3) genome copies of OvHV-2 DNA. An uninfected control lamb was housed with the animals exposed to secretions containing 101 copies. All animals were sampled bi-weekly from 4 to 60 days p.i. Animals nebulized with inocula containing 102 and 101 copies and the control lamb were then sampled weekly (67 to 120 days p.i.). The lamb receiving inoculum containing 104 copies developed detectable viral DNA in PBL by 14 days p.i. and virus-specific antibody by 25 days p.i. (Table 1). Lambs nebulized with secretions containing 103 copies developed detectable OvHV-2 DNA by 1421 days p.i. (Table 1
). Virus-specific antibody was detectable by 28 and 32 days p.i. (Table 1
). One animal nebulized with inoculum containing 103 copies became seronegative by 46 days p.i., but was positive again by 60 days p.i. and remained positive until the termination of the experiment (data not shown). The lambs nebulized with secretions containing 102 and 101 copies of OvHV-2 and the control lamb did not develop detectable viral DNA or virus-specific antibody at any time during the experiment (Table 1
). Based on these results the minimum infectious dose for this pool of nasal secretions is between 1000 and 100 copies of viral DNA. Genome copy number does not directly measure the number of infectious virions. Therefore, infectivity of every new pool of nasal secretions will need to be determined empirically.
Sheep-associated MCF is emerging as a significant source of economic loss in several ruminant species, particularly farmed bison and deer (O'Toole et al., 2002; Reid, 1992
). Currently no vaccine is available to control the disease and the pathogenesis of MCF is poorly understood. Given the lack of an in vitro propagation system for OvHV-2, another means for obtaining infectious virus for use in animal studies, including vaccine development, is needed. In this study, we have defined the use of nasal secretions from sheep as a source of infectious OvHV-2 and determined the minimum infectious dose of the pool of nasal secretions. Controlled studies of the pathogenesis of MCF in clinically susceptible species, such as bison, and vaccine development are now possible.
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ACKNOWLEDGEMENTS |
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Received 20 October 2004;
accepted 6 December 2004.
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