Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK1
Unité de phytopathologie, Université catholique de Louvain, Louvain-la-Neuve, Belgium2
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru (PO), 502 324 Andhra Pradesh, India3
Author for correspondence: Claude Bragard. Fax +32 10 47 86 97. e-mail bragard{at}fymy.ucl.ac.be
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Abstract |
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Introduction |
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Expression of CP genes in bacteria or in plants can give rise to virus-like particles, as shown for viruses as different as Alfalfa mosaic virus (Yusibov et al., 1996 ), Cowpea chlorotic mottle virus (Zhao et al., 1995
), Cowpea mosaic virus (Wellink et al., 1996
) and the potyviruses, Johnsongrass mosaic virus and Potato virus Y (Jagadish et al., 1991
, 1993
; Stram et al., 1993
). VLP of Tobacco mosaic virus (TMV) CP were detected in both transgenic plants (Sleat et al., 1989
) and Escherichia coli (Hwang et al., 1994
), if the encapsidated RNA contained an origin of assembly sequence (OAS). The assembly of VLP in either plants or bacteria has been proposed as a way to protect and accumulate specific mRNA (Sleat et al., 1989
), as a means to study the molecular assembly of the capsid (Jagadish et al., 1991
; Hwang et al., 1994
) or for the production of oral vaccines (Mason et al., 1996
). In this paper, we report the occurrence of VLP in transgenic plants expressing the 5'-most sequence of IPCV-H RNA-2 and in cells of E. coli expressing the IPCV-H CP gene.
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Methods |
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Purification of IPCV particles and IPCV-like particles.
Virus particles were purified from N. benthamiana plants infected by mechanical inoculation as described by Reddy et al. (1985) and were stored at -20 °C in 0·06 M NaCl, 0·01 M TrisHCl, 0·003 M EDTA. Infectivity was assessed by mechanical inoculation on N. benthamiana, Chenopodium amaranticolor and Phaseolus vulgaris cv. Topcrop. The same protocol was used in attempts to purify VLP from ca. 200 g batches of F2 transgenic plants. Fractions obtained after centrifugation through a sucrose cushion, as well as after a sucrose gradient step were assessed by ELISA and immunosorbent electron microscopy (ISEM).
Extraction of virus-like particles from E. coli.
Cells of E. coli BL21(DE3)pLysS were transformed with the plasmid pET-15b (Novagen, AMS biotechnology) containing the IPCV-H coat protein gene (nucleotides 5031129, i.e. lacking non-coding sequences). After an overnight preculture, cells were grown in 20 ml LB+ampicillin at 37 °C for 4 h, before induction with 1 mM IPTG. Cells were then collected by centrifugation (6000 g, 4 °C, 10 min), resuspended in 1 ml 10 mM TrisHCl, 1 mM EDTA, pH 8 (TE) and stored at -20 °C for further use. Samples of the suspension were denatured and subjected to SDSPAGE [15% (w/v) gel] as described by Reddy & Mayo (1985) . After electrophoresis and staining in Ponceau S (1%), proteins were transferred to nitrocellulose and probed with IgG from a rabbit antiserum specific for IPCV-H at 1 µg/ml followed by goat anti-rabbit IgG conjugated to alkaline phosphatase (Sigma) and colorimetric reaction. Controls included a purified preparation of IPCV-H and an extract from an IPCV-H-infected N. benthamiana.
Electron microscopy.
For electron microscopy, frozen suspensions of partially purified VLP were thawed on ice and centrifuged at 4 °C at 6000 g for 10 min to remove insoluble material. The remaining solution was kept on ice and sonicated. The resulting mixture was used for ISEM.
ISEM and antibody coating (AC) of antibody-trapped VLP were done as described by Roberts (1986) . Antibodies to IPCV-H were diluted in 0·06 M phosphate buffer, pH 6·5. Grids were negatively stained with 2% sodium phosphotungstate, pH 6 and were examined in a Philips CM-10 or a JEOL 1200 Ex electron microscope.
Immunocapture (IC)RTPCR.
Plants were selected as being transgenic by PCR using primers designed to amplify the IPCV-H CP gene (nucleotide position 1141, 5' AGACGTCGACATTAATTCACCC 3', and nucleotide position 496, 5' ACGGACATGTCTAATTTGTCTG 3').
ICRTPCR experiments were done on transgenic N. benthamiana plant extracts as described by Brandt & Himmler (1995) . PCR tubes were coated with 100 µl anti-IPCV-H IgG (1 µg/ml) in 0·1 M carbonate buffer, pH 9·6. After 2 h incubation, tubes were washed twice in PBS0·05% Tween and twice in PBS. Plant extracts were made in phosphate buffer (0·01 M, pH 6·5) using a 1:10 leaf/buffer ratio (w/v). Extracts were centrifuged to remove plant debris and 100 µl samples were incubated overnight at 4 °C in the coated tubes. Tubes were then washed three times with 200 µl PBSTween and three times with 200 µl PBS. For assays, tubes were washed by vortexing with 10 µl Triton X-100 in TE and eight samples were subjected to the two-step RTPCR done according to manufacturers instructions (Expand reverse transcriptase, Expand high fidelity, Boehringer Mannheim). The primers already described for PCR were also used for the ICRTPCR. As a control, the same plants were submitted to an ICRTPCR using primers designed to amplify the 250 bp from the 3' end of both RNA-1 and RNA-2 of IPCV.
Inoculation of IPCV-L virus in transgenic N. benthamiana expressing the IPCV-H CP gene.
To increase the number of particles available for analysis, the transgenic N. benthamiana line AC20 was inoculated with plant sap of a freezer-stored isolate of IPCV-L serotype (Nolt et al., 1988 ). Plants were then tested for the presence of particles by ISEM and immunodecoration using an anti-IPCV-H antiserum.
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Results |
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VLP were detected in some extracts of AC20 plants, but not those of AC14. Changing the pH or composition of the extraction buffer did not result in better detection of VLP. The VLPs resembled particles of IPCV in diameter and in having a central hole, but were markedly shorter (Fig. 1A).
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To test for the presence of the CP gene in the VLPs, plant extracts were assayed by ICRTPCR using primers chosen to amplify the CP gene. Of the eight AC20 transgenic plants tested, seven yielded a ca. 600 bp fragment that corresponded in size to the CP gene (Fig. 2). To exclude IPCV particles as the source of the PCR product, ICRTPCR was done using primers designed to amplify the conserved 3' end of RNA-1 and RNA-2 of IPCV. Extracts of IPCV-H-infected N. benthamiana yielded the predicted product, but no amplification product was obtained from extracts of the transgenic plants.
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Expression of CP in bacteria
E. coli BL21(pLysS) cells were transformed with pET15b containing IPCV-H CP. After induction of expression the levels of the CP were monitored over a 20 h period by immunoblotting. The CP was readily detectable in samples taken after 1 h and did not change much after longer induction periods (Fig. 3).
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Discussion |
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Little is known about the assembly of particles of IPCV and similar viruses such as Beet necrotic yellow vein virus (BNYVV) or Potato mop-top virus. Similarities in amino acid sequence among CPs of viruses with rod-shaped particles suggests that their assemblies share a common mechanism (Goulden et al., 1992 ). Thus the assembly mechanism for TMV CP could suggest a mechanism for IPCV. However, TMV particle assembly is initiated by the interaction between an origin of assembly sequence (OAS), a 75 nucleotide sequence that can form a hairpin loop, which interacts with a two-layer disk-like structure (reviewed in Butler & Mayo, 1987
). The results of ICRTPCR and particle length measurements indicate that IPCV CP encapsidates the transcript RNA that encodes the CP in both prokaryotic and eukaryotic cells. Thus if IPCV RNA has an OAS, it lies within the CP gene. However, as with other viruses with more than one genome RNA, the OAS does not seem to be a particular nucleotide sequence as no common sequence was detected between RNA-1 (Miller et al., 1996
) and the CP gene (Wesley et al., 1994
) of IPCV-H. For example, with BNYVV Gilmer et al. (1992)
have shown that a sequence of the non-coding 5' end of BNYVV RNA-3 is required for RNA packaging in vivo although there is no similarity between this sequence and that of the other BNYVV RNAs. An alternative explanation is suggested by the work of Guilley et al. (1975)
, who showed that TMV CP could encapsidate sequences other than the OAS. A specifically encapsidated fragment (SERF) was found in the CP gene that was thought probably to be responsible for the encapsidation of RNA of Sunn-hemp mosaic virus (Hirth & Richards, 1981
).
VLP were difficult to detect in transgenic plants, especially of line AC14. VLP were more readily detected in bacterial extracts, which indicates that assembly does not involve post-translational modification. Conditions for in vitro assembly of VLPs differ greatly from one virus to another, as for example for aggregation of CPs of TMV and Tobacco rattle virus (Mayo et al., 1993 ). Nevertheless, it is not yet clear at this stage how frequent is the assembly phenomenon, and under what conditions VLP are assembled inside the cells or during the processing of the cells extracts.
To our knowledge no VLP formation has been reported for any plant viruses transmitted by fungus. When extracts of transgenic plants that were expressing large amounts of PMTV CP (Barker et al., 1998 ) were searched for VLP, none was found (unpublished results).
The occurrence of VLP in transgenic plants raise questions about risks linked to heteroencapsidation, a phenomenon deduced to occur with other viruses for which VLP have been observed, such as potyviruses, from the results of challenge inoculation of transgenic plants (Jacquet et al., 1998 ; Hammond & Dienelt, 1997
). We have observed a type of heteroencapsidation in the experiments reported here. Particles were formed that corresponded in length to particles containing RNA of IPCV-L, but patches of the protein coats of these particles consisted of CP of IPCV-H. This shows that IPCV-H CP is able to interact with IPCV-L RNA, and the pattern of patches of heterologous CP suggests that once IPCV-H CP had started to encapsidate the RNA, it was more likely to interact with itself than with the more abundant IPCV-L CP, and vice versa.
These results are another example of genomic masking as demonstrated by Rochow (1977) for barley yellow dwarf viruses. If, as with luteoviruses, vector transmission of IPCV depends on surface features of the virus particles, such masking could result in a change in vector specificity for the masked virus genome, and hence altered pathogenicity for this virus. It is not known if the IPCVPolymyxa graminis interaction does involve the CP, but our results suggest a potential problem that should be considered if and when transgenes based on the CP gene of IPCV are used in attempts to obtain transgenic resistance.
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Acknowledgments |
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Footnotes |
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c Present address: Department of Plant Pathology, University of Georgia, Athens, GA 30602, USA.
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References |
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Received 16 August 1999;
accepted 11 October 1999.