1 Department of Virology, Faculty of Medicine, Imperial College London, St Mary's Campus, Norfolk Place, London W2 1PG, UK
2 LICR, Experimental Medicine Unit, Université Catholique de Louvain, Brussels, Belgium
3 Department of Biochemistry & Molecular Biology, New Jersey Medical School, Newark, USA
4 Department of Genetics, Harvard Medical School, Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
Correspondence
Geoffrey L. Smith
glsmith{at}imperial.ac.uk
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ABSTRACT |
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INTRODUCTION |
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The IL-10 family of proteins includes IL-10 and other cytokines with structural similarity such as IL-19 (Gallagher et al., 2000), IL-20 (Blumberg et al., 2001
), IL-22 (Dumoutier et al., 2000
), IL-24 (Jiang et al., 1995
; Caudell et al., 2002
) and IL-26 (Knappe et al., 2000
). Several herpesviruses and poxviruses encode proteins related to the IL-10 family. Human herpesvirus 4 (EpsteinBarr virus) and Orf virus express proteins that are closely related to IL-10 from their human or ovine host (Moore et al., 1990
; Fleming et al., 1997
; Kotenko et al., 2000
) and viral proteins that are more distantly related to IL-10 are encoded by human and primate cytomegalovirus (Kotenko et al., 2000
; Lockridge et al., 2000
) and YLDV (Lee et al., 2001
). Collectively, these mammalian and virus proteins form the IL-10-related cytokine family (Kotenko, 2002
; Renauld, 2003
).
The functions of IL-10-like cytokines are only partially understood. Human IL-24 expression diminishes in melanocytes with tumorigenesis and melanoma progression (Jiang et al., 1995), but is restored during induction of growth arrest and terminal differentiation (Huang et al., 2001
). Overexpression of IL-24 can induce apoptosis in several human cancer cell lines (Jiang et al., 1996
; Su et al., 1998, 2003
; Kawabe et al., 2002
; Lebedeva et al., 2002
) and may be linked with p38 mitogen-activated protein kinase activity and upregulation of growth arrest and DNA damage-inducible genes (Sarkar et al., 2002
; Sauane et al., 2003
).
IL-24 is a secreted glycoprotein (Wang et al., 2002) expressed by cells of the immune system (Schaefer et al., 2001
; Garn et al., 2002
). The receptors for IL-24 and other IL-10-related cytokines are members of the class II cytokine receptor family, a group of heterodimeric, transmembrane proteins (Renauld, 2003
). IL-19, IL-20 and IL-24 utilize the IL-20R type 1 that consists of IL-20R
(long-chain) and IL-20R
(short-chain) subunits. IL-24 and IL-20 also bind to the IL-20R
subunit complexed with the long-chain IL-22R subunit, designated IL-20R type 2 (Dumoutier et al., 2001b
; Wang et al., 2002
). Upon receptor engagement these cytokines activate the Janus kinase (JAK)/signal transducers and activators of transcription (STAT) signal transduction pathway, resulting in phosphorylation, nuclear translocation and transcriptional activity of STAT3 and STAT1 (Dumoutier et al., 2001b
; Parrish-Novak et al., 2002
).
The immunological functions of IL-10-related cytokines are unclear. Transgenic mice overexpressing either human or murine IL-20 showed hyperproliferation and abnormal differentiation of skin cells. Furthermore, STAT-driven luciferase expression in response to IL-20 was enhanced by the proinflammatory cytokines IL-1 and tumour necrosis factor (TNF)-
, indicating a role for IL-20 in the inflammatory response in skin (Blumberg et al., 2001
). The murine counterpart of IL-24, designated IL-4-induced secreted protein, is secreted by CD4+ T cells under Th2 differentiation conditions (Schaefer et al., 2001
), whilst the rat counterpart (mob-5 or C49a) affects Ras-mediated neoplasia (Zhang et al., 1997
) and proliferation of fibroblasts during wound healing (Soo et al., 1999
). Human PBMC can secrete IL-19, IL-20 and IL-24; however, the distribution of receptors for these cytokines suggested that immune cells may not be their primary target (Wolk et al., 2002
). Recently, IL-24 was reported to inhibit angiogenesis and endothelial cell differentiation via the STAT3 signal transduction pathway following activation of the IL-22R (Ramesh et al., 2003
).
This study has characterized the IL-10-related cytokine Y134R encoded by YLDV. Bioinformatic analyses and protein modelling predicted Y134R to be an -helical glycoprotein most closely related to IL-24. Data presented show that Y134R is secreted from cells and stimulates the class II cytokine receptors IL-20R type 1 and IL-20R type 2 to induce the JAK/STAT signal transduction pathway, as measured by STAT phosphorylation, nuclear translocation and reporter gene activation. Expression of this viral protein reduced vaccinia virus (VACV) virulence in a murine infection model.
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METHODS |
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YLDV was obtained from the ATCC, propagated in OMK cells and purified as described (Lee et al., 2001). The VACV strain Western Reserve (WR) lacking the B8R gene (v
B8R) (Symons et al., 2002
) and recombinants thereof were grown and titrated on BS-C-1 cells.
Transfections and protein expression by mammalian cells.
Y134Rflag was generated by PCR using purified YLDV genomic DNA as template and oligonucleotides (5'-GCACGAATTCAATATATAACAAAATG-3' and 5'-ATATTCTAGATTACTTATCGTCGTCATCCTTGTAATCACCTCCACCATTTTTGCCATAACCCATG-3') that contained EcoRI and XbaI restriction sites (underlined) and a flag tag Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (bold) followed by a stop codon. The PCR product was digested with EcoRI and XbaI and cloned into mammalian expression plasmid pCI (Promega) forming pCI-Y134Rflag. Recombinant pCEP4 plasmids encoding human C-terminal flag-tagged IL-19, IL-20, IL-22 and IL-24 (Dumoutier et al., 2001b) and expression plasmids encoding class II cytokine receptor subunits IL-20R
, IL-20R
, IL-22R and IL-10R
(Wang et al., 2002
) have been described. For analysis of STAT3 phosphorylation and nuclear translocation, Y134R and IL-10-related cytokines were expressed transiently in HEK293T cells. IL-20 and Y134R were expressed transiently in HEK293-EBNA cells as described (Dumoutier et al., 2001b
). Recombinant human IL-22 was produced in Escherichia coli (Dumoutier et al., 2001a
) and recombinant IFN-
1/IL-29 was produced as described (Kotenko et al., 2003
).
Production of rabbit polyclonal antiserum against Y134R.
For expression in E. coli the 134R gene encoding an N-terminal (6)His tag was generated by PCR using pCI-Y134Rflag plasmid DNA as template and primers (5'-GTAACACATATGTTAAATTGTGGAATAGAACAC-3' and 5'-TTTTAAGCTTTTAAATTTTTGCCATAACCC-3') that contained NdeI and HindIII sites (underlined). The product was digested with these enzymes and cloned into pET28a (Novagen) to form pET28-HisY134R. This plasmid was introduced into B834(DE3) cells (Novagen) and HisY134R was expressed in inclusion bodies by addition of 1·0 µM IPTG for 3 h at 37 °C. These were released in BugBuster protein extraction reagent, isolated as instructed by the manufacturer (Novagen) and solubilized in 20 mM Tris/HCl, 0·5 M NaCl, 5 mM imidazole, 6 M guanidine hydrochloride, 1 mM 2-mercaptoethanol. HisY134R was purified by Ni2+ affinity chromatography (HiTrap chelating HP column; Amersham Pharmacia Biotech) under denaturing conditions according to the manufacturer's instructions. The purified protein (100200 µg) was injected into New Zealand White rabbits to produce anti-Y134R serum (Harlan Sera Labs) from which the Ig fraction was purified by affinity chromatography on a HiTrap protein ASepharose column (APB) as instructed by the manufacturer.
Immunoblotting.
Cells were infected or mock-infected with virus at 10 p.f.u. per cell in the presence, where indicated, of 40 µg -D-arabinofuranoside (araC) ml1, 1 µg tunicamycin ml1 or 1 µM monensin (all from Sigma). Alternatively, cells were stimulated with 10 % conditioned supernatant from HEK293T cells transfected with empty vector or plasmid encoding Y134R or other cytokines. At various times post-infection/stimulation cells were harvested directly into reducing SDS-PAGE loading buffer. Supernatants were centrifuged (2500 g, 10 min) to remove cellular debris, filtered through a 0·1 µm filter and then concentrated in a centrifugal filter device (Amicon). Where indicated, deglycosylation of N-linked carbohydrate from proteins in supernatants was achieved using peptide-N-glycosidase F (PNGase F) (Prozyme) according to the manufacturer's instructions. Proteins in cell extracts and supernatants were resolved by SDS-PAGE and analysed by immunoblotting with either mouse
-flag mAb (Sigma), rabbit
-Y134R IgG, mouse
-phosphoSTAT3 mAb or rabbit
-phosphoSTAT1 IgG (each from Upstate Biotechnology and diluted 1 : 1000) as described (Bartlett et al., 2002
).
Construction of a recombinant VACV expressing Y134R.
A recombinant VACV expressing Y134R under the control of a strong synthetic early and late promoter (pSEL) from the B8R locus of the vB8R virus was constructed by transient dominant selection using the Ecogpt selectable marker (Boyle & Coupar, 1988
; Falkner & Moss, 1990
). The Y134R ORF was amplified by PCR using pCI-Y134flag as DNA template and oligonucleotides 5'-CATTCTCGAGGCCACCATGAAATTATACTTTTATTGTATT-3' and 5'-TTACATCGATTTAAATTTTTGCCATAACCCATG-3', which contained XhoI and ClaI sites (underlined). The product was digested with these enzymes and cloned into the p
B8R plasmid (Symons et al., 2002
) downstream of the VACV pSEL forming the plasmid p
B8RSEL-Y134R. This was transfected into v
B8R-infected CV-1 cells and, following resolution of a mycophenolic acid-resistant intermediate virus, Y134R-expressing (v
B8R-Y134R) and parental control viruses were isolated. A revertant virus (v
B8R-Rev) was created by transfecting p
B8R into v
B8R-Y134R-infected cells. Generation and characterization of v
B8R-Y134R were carried out under the biological containment conditions and procedures required by the UK Health and Safety Commission's Advisory Committee on Genetic Modification and with their notification.
Immunofluorescence.
BHK-21 cells on 13 mm glass coverslips were transfected with plasmids encoding class II cytokine receptor subunits. At 16 h post-transfection cells were starved in serum-free medium for 6 h before being stimulated for 20 min with conditioned HEK293T supernatants (10 % of overlay volume) containing Y134R or IL-10-related cytokines. Cells were fixed in 4 % paraformaldehyde (PFA) in 250 mM HEPES for 20 min on ice. Following quenching for 15 min on ice with 20 mM glycine in PBS, cells were permeabilized and blocked with 0·1 % Triton X-100 in PBS containing 10 % FBS for 20 min at room temperature. Cells were washed with PBS and then incubated with -STAT3 rabbit IgG diluted 1 : 200 in PBS containing 10 % FBS for 1 h at room temperature followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (both from Cellomics). Cells were washed three times in PBS containing 10 % FBS and once in water before mounting in Mowiol as described previously (Sanderson et al., 1996
). Samples were examined with an LSM 5 Pascal attached to an Axioplan 2 imaging microscope (Zeiss) and images were captured and prepared using Zeiss LSM image browser software version 3.2.
Luciferase assays.
HT-29 cells were electroporated (107 cells in 400 µl, 250 V, 192 , 1200 µF) with 15 µg of pGRR5, pRL-TK and IL-20R
cDNA cloned as described (Dumoutier et al., 2001b
). BW-LICR2 cells were electroporated (107 cells in 800 µl, 270 V, 74
, 1200 µF) with 15 µg of pGRR5 and pRL-TK. Transfected cells were seeded in 24-well plates and incubated for 5 h at 37 °C to allow the expression of IL-20R
. The cells were stimulated with the indicated cytokine for 2 h before being collected by centrifugation and lysed. Both luciferase activities were monitored with the Dual Luciferase Reporter Assay System kit (Promega) according to the manufacturer's instructions.
Assay for virus virulence.
The virulence of vB8R-Y134R compared with control viruses was assessed in female BALB/c mice (68 weeks old) as described (Alcami & Smith, 1992
). To assess virus titres in organs, mice were sacrificed at the indicated times post-infection. Lungs and brains were removed and homogenized by forcing through a 70 µm nylon cell strainer. Organ homogenates were freezethawed three times and sonicated before being assayed for infectious virus by plaque assay on BS-C-1 cells.
Bioinformatic analyses and protein modelling.
Alignments were done using CLUSTAL W (Thompson et al., 1994) and formatted using GeneDoc (Nicholas, 1997
). The hydrophobicity profile of the protein sequence was created using the program ProtScale (Kyte & Doolittle, 1982
). Secondary and three-dimensional structure models were predicted from amino acid sequences by local structure PsiPred (Jones, 1999
) and 3D-PSSM fold recognition methods (Kelley et al., 1999
, 2000
). A structural model of Y134R was based on sequence alignments and recognized folds, using the Swiss-PdbViewer program for alignment (version 3.7; www.expasy.ch/spdbv/) and the optimize mode of the SWISS-MODEL program server (version 3.5; www.expasy.ch/swissmod/SWISS-MODEL.html) for automatic model building (Peitsch, 1995
; Guex & Peitsch, 1997
; Schwede et al., 2003
). The Y134R structure was modelled using IL-19 (pdb i.d. 1n1f) as a template (Chang et al., 2003
). Several cycles of energy minimization were performed to optimize bond geometry and to improve the stereochemistry of the final model using the optimize mode using the Swiss-PdbViewer implementation of the GROMOS96 43B1 force field, together with the SWISS-MODEL server minimization protocol. The quality of the models was accessed using the WhatCheck program (Hooft et al., 1996
). Identification of secondary structure elements, calculation of the electrostatic surface potential and visualization were made using the program PyMol (DeLano, 2002
).
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RESULTS |
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The Y134R gene encodes a secreted protein
To characterize Y134R, a C-terminal flag-tagged copy of the Y134R gene was transfected into HEK293T cells. For comparison, flag-tagged cDNAs encoding IL-19, IL-20, IL-22 and IL-24 were expressed in parallel. Supernatants from all transfections were analysed by immunoblotting with a mAb that recognizes the flag epitope (Fig. 2a). HEK293T cells secreted Y134Rflag as two different forms, which probably represent differences in glycosylation, whilst the other cytokines were expressed as reported previously (Dumoutier et al., 2001b
). To compare Y134Rflag with the untagged Y134R made by YLDV- or v
B8R-Y134R-infected cells, the rabbit anti-Y134R polyclonal IgG was used in immunoblotting (Fig. 2b
). Y134R was secreted from infected cells as two different forms that were approximately 13 and 2022 kDa, slightly smaller than those observed for Y134Rflag. The predicted size of mature Y134R is 16·1 kDa; thus it is likely that the diffuse 2022 kDa band is glycosylated. The smaller form did not exactly match the predicted size for unknown reasons. Expression from VACV yielded significantly higher levels of Y134R than from YLDV, consistent with the use of a strong VACV promoter.
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Y134R is a monomer that requires glycosylation for secretion
To determine whether Y134R is a monomer, dimer or oligomer, supernatants from vB8R-Y134R-infected cells were analysed by size-exclusion chromatography as described previously (Bartlett et al., 2002
). Y134R eluted from the column with a size consistent with it being monomeric under physiological conditions (Fig. 3
a).
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Identification of the class II cytokine receptors utilized by Y134R
Given the predicted structural similarity shared by Y134R and IL-10-related cytokines, we investigated whether Y134R could signal via receptors used by these cytokines. The human HaCaT keratinocyte cell line (Boukamp et al., 1988) expresses both IL-20 receptors that signal via STAT3 upon ligand binding (Blumberg et al., 2001
). To assess whether Y134R could activate this signal transduction pathway, HaCaT cells were treated with conditioned supernatants with or without Y134Rflag and the level of STAT3 phosphorylation was compared with that induced by treatment with supernatants containing different human cytokines (Fig. 4
a). After 20 min incubation with Y134R or each other cytokine tested, there was a significant increase in the level of phosphorylated STAT3 above that induced by incubation with supernatants taken from cells transfected with empty vector (pCI).
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Y134R activates the JAK/STAT signal transduction pathway
Following specific ligandreceptor interaction at the cell surface, STATs become phosphorylated on tyrosine residues, dimerize and enter the nucleus to induce transcription of responsive genes (Darnell, 1997). Therefore, the location of STAT3 was investigated in cells expressing the IL-20R type 2 (Fig. 5
ac) or the IL-22R (Fig. 5d, e
). BHK-21 cells expressing transiently the IL-20R type 2 were stimulated with conditioned HEK293T supernatants containing IL-19, IL-24 or 134R (Fig. 5a
c). In agreement with the immunoblotting data (Fig. 4
), both IL-24 and Y134R activated STAT3 in cells expressing this IL-20 receptor, resulting in translocation of STAT3 to the nucleus. In contrast, IL-19 was unable to activate STAT3 via the IL-20R type 2 and no nuclear localization of STAT3 was apparent. To analyse further whether Y134R could induce STAT3 activation via the IL-22R, cells expressing this receptor were stimulated with either IL-22 or Y134R (Fig. 5d, e
). As expected, IL-22 induced nuclear localization of STAT3 in these cells whereas Y134R did not.
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Expression of Y134R reduces VACV virulence
Having demonstrated a biological activity of 134R in vitro, we next examined the contribution of Y134R to the virulence of a poxvirus. Because YLDV does not induce disease in mice and no primate model was available, we sought to examine a role for Y134R via its expression in VACV. Y134R was expressed from a VACV strain WR mutant engineered to lack the VACV IFN- binding protein, B8R. This protein does not affect VACV virulence in a murine intranasal model because it does not inhibit mouse IFN-
(Symons et al., 2002
), but this virus would be predicted to be attenuated in humans because the VACV B8R protein does inhibit human IFN-
(Alcami & Smith, 1995
; Mossman et al., 1995
). Therefore, this strain was selected as a potentially safer parent virus from which Y134R could be expressed. The parent virus v
B8R, the virus expressing Y134R (v
B8R-Y134R) and a revertant virus from which the Y134R gene was removed (v
B8R-rev) were used to infect BALB/c mice intranasally. The outcome was assessed by monitoring body weight and signs of illness as described previously (Alcami & Smith, 1992
). Mice infected with v
B8R-Y134R lost less weight and presented milder disease symptoms than mice infected with control viruses (Fig. 7
a, b). The differences were significant for weight loss (P<0·02) from day 7 and for signs of illness (P<0·05) from day 6 post-infection. To assess virus titres in vivo, lungs and brain were harvested on days 4, 6 and 9 post-infection and tissue homogenates were assayed for infectious virus by plaque assay (Fig. 7c
). In v
B8R-Y134R-infected lungs on days 4 and 9 the amount of infectious virus was significantly less (P<0·05) than that observed in lungs from controls. Virus titres in brains from v
B8R-Y134R-infected mice were also lower than controls at each time point, although these differences were not statistically different due to sample variation, particularly on days 4 and 9. It should be noted that on both of these days, two out of five mice from the v
B8R-Y134R-infected group had no detectable virus in the brain (<40 p.f.u.). In contrast, all mice in the control virus-infected groups had detectable virus in the brain on all days tested.
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DISCUSSION |
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Y134R is most like murine IL-24 with 30 % identity and 54 % amino acid similarity. In addition, there are conserved cysteines characteristic of this family of cytokines (Fig. 1). Features of Y134R include a signal peptide and a largely
-helical domain containing conserved cysteines and a site for attachment of N-linked carbohydrate. These features are conserved in the IL-19, IL-20 and IL-24 cytokines. Modelling the YLDV protein using the known structure of IL-19 revealed a similar topology, but Y134R has some flexible loops near the N terminus. Two Y134R cysteines, equivalent to C10 and C57 in IL-19, might form an intracellular disulphide bond, although these form SS bonds with other cysteines in IL-19 (Chang et al., 2003
). In IL-24 these cysteines have been suggested to form either an intramolecular SS bond or to form a disulphide-bonded dimer (Chang et al., 2003
). The oligomeric status of Y134R was difficult to predict from these analyses, but size-exclusion chromatography revealed that Y134R was predominantly monomeric. Whilst the conformation of IL-24 is unknown, both IL-19 and IL-20 are monomeric in solution and form ternary 1 : 1 : 1 complexes with the IL-20R type 1 (Pletnev et al., 2003
).
Y134R was secreted from cells as a 2022 kDa glycoprotein and this required N-linked glycosylation since secretion was blocked in the presence of tunicamycin. Treatment of the secreted form with PNGase F reduced the protein size to approximately 13 kDa. Like IL-20 and IL-24, Y134R induced STAT activation via IL-20R types 1 and 2. Therefore, Y134R is the first viral IL-10-related protein found to induce signalling via class II cytokine receptors, other than the IL-10 receptor. Given that class II cytokine receptor complexes share subunits, and some ligands such as IL-20 and IL-24 are able to bind more than one receptor complex, we tested whether Y134R competitively blocked signalling from other class II cytokine receptors, such as the IL-22R which contains the same -subunit as IL-20R type 2. However, pre-incubating IL-22R-expressing cells with Y134Rflag before stimulating with a non-saturating concentration of IL-22 did not reduce luciferase production from these cells (data not shown), suggesting that Y134R does not antagonize the IL-22R. A similar experiment was done with cells expressing IFN-
/IL-28R. Again, pre-incubation with Y134Rflag had no effect on luciferase production induced by stimulation with IFN-
/IL-29 (data not shown). Preferential phosphorylation of STAT3 versus STAT1 was observed in cells expressing either IL-20Rs type 1 or 2 following stimulation with Y134R. This is consistent with previous reports for IL-20 and IL-24 (Parrish-Novak et al., 2002
) and further links the functional relationship of Y134R to these cytokines.
Mice infected intranasally with a VACV expressing Y134R lost less weight and presented milder disease symptoms compared with those observed for control virus-infected mice. In addition, virus titres were lower in lungs and brains when Y134R was expressed. Taken together, these results indicated that expression of Y134R by VACV caused virus attenuation in a murine intranasal model. A reduction in virus virulence in this model was also reported for a recombinant VACV (WR strain) expressing the 35 kDa chemokine-binding protein from VACV Lister strain (Reading et al., 2003). The attenuated phenotype was associated with a reduction in the cellular inflammatory response in the lungs due to inhibition of CC chemokine chemotactic activity.
Recombinant poxviruses expressing cytokines that promote a Th1-type immune response, inflammation or antiviral activity such as IL-2 (Ramshaw et al., 1987), IFN-
(Kohonen-Corish et al., 1990
), TNF-
(Sambhi et al., 1991
), IL-12 (Gherardi et al., 1999
) and IL-18 (Gherardi et al., 2003
) are also attenuated compared with control viruses. In contrast, recombinant viruses expressing Th2-type cytokines such as IL-4 exhibited a significant increase in virulence (Andrew & Coupar, 1992
; Bembridge et al., 1998
; Jackson et al., 2001
). Such studies have demonstrated the importance of innate immunity and subsequent development of the appropriate Th response for effective clearance of poxvirus infections (Ramshaw et al., 1992
). One possibility to explain the attenuated phenotype of the Y134R-expressing VACV is that Y134R possesses immunostimulatory activity and consequently induces a more effective antiviral response in this model.
The biological role of Y134R during YLDV infection of the natural host might be different to that reported here in the mouse intranasal model. With IL-24 it was suggested that the functions of the mouse and rat orthologues within their respective hosts may be different to that of IL-24 in humans (Kotenko, 2002); however, this needs systematic comparison in the same experimental system. The reported biological activities of IL-24 derive mostly from models using recombinant adenoviruses and tumour growth, and the phenotype of an IL-24 knockout transgenic mouse is unknown. The mouse intranasal model used here induces a systemic infection and has been used to study VACV virulence and the contribution of specific virus proteins to virulence (Williamson et al., 1990
; Tscharke et al., 2002
; Reading & Smith, 2003
). Intradermal infection of the mouse ear pinnae has also been used to study VACV pathogenesis (Tscharke & Smith, 1999
) and notably the presence or absence of a specific gene could cause differing outcomes depending on which infection model was used (Tscharke et al., 2002
). Therefore, the role of Y134R in other infection models is also being investigated.
In summary, data presented show that Y134R is a secreted glycoprotein that has biological activity in vitro, where it can stimulate signal transduction via the IL-20 type 1 and 2 receptors and in vivo where it causes the attenuation of VACV in a murine intranasal model.
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ACKNOWLEDGEMENTS |
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Received 15 January 2004;
accepted 18 February 2004.