Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
Correspondence
Malcolm A. McCrae
mmccrae{at}bio.warwick.ac.uk
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ABSTRACT |
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Rotavirus strains (human virus isolates Wa G1P[8], DS1 G2P[4], Hochi G4P[8], ST3 G4P[6], 69M G8P[10], WI-61 G9P[8], A64 G10P[?] and L26 G12P[4]; porcine isolates OSU G5P[7] and YM1 G11P[7]; bovine isolate UKtc G6P[5]; equine isolate L338 G13P[?]; avian isolate CH-2 G7P[?]) were all grown in African green monkey kidney cells (BSC-1) after pre-treatment with 10 µg trypsin ml-1 as described previously (McCrae & Faulkner-Valle, 1981). Total cytoplasmic RNA was extracted from infected cells as described previously (Johnson & McCrae, 1989
). The gene encoding VP3 from each of these isolates was subject to RT-PCR using primers specific for the first 25 bases at the 5' termini and the final 26 bases at the 3' termini of RNA segment 3 derived from the human KU G1P[8] strain sequence present in GenBank (accession no. AB022767). The sequences of the primers used were: 5' end primer, 5'-GGCTATTAAAGCAGTACTAGTAGTG-3'; 3' end complementary primer, 5'-GGTCACATCATGACTAGTGTGTTAAG-3'. RT-PCR was carried out using either a single reaction mixture (Xu et al., 1990
) or the more traditional two-step approach, in which an aliquot of the reverse-transcribed viral RNA was added to a separate reaction mixture for the PCR step (Ball, 2000
). In some cases, the Qiagen One-Step RT-PCR kit was used according to the manufacturer's instructions for long template regions. The
2·6 kbp PCR amplicons were isolated from 1 % agarose gels and cloned into the plasmid pCR2.1 using the TA cloning kit (Invitrogen) according to the manufacturer's instructions. Sequencing of the viral gene cloned in pCR2.1 was carried out using the Big Dye Terminator version 3.1 chemistry (Applied Biosystems) with the fragments being resolved on an ABI Prism 3100 Genetic Analyzer. In order to exclude sequence errors introduced by Taq DNA polymerase, at least three independent clones were sequenced for each virus isolate. The consensus VP3 sequences obtained were deposited in GenBank and given the following accession numbers: Wa, AY267335; DS1, AY277914; Hochi, AY277915; ST3, AY277919; 69M, AY277916; WI-61, AY277917; A64, AY277920; L26, AY277918; OSU, AY277921; YM1, AY300922; UKtc, AY300923; L338, AY277922; and CH-2, AY277923. Other sequences already in GenBank included SA11 G3P[2], X16062; RF G6P[?], AY116592; and PO-13 G7P[?], AB009631. The deduced amino acid sequences were aligned using CLUSTAL W (Thompson et al., 1994
) at NPS@:Network Protein Sequence Analysis (Combet et al., 2000
).
The RT-PCR strategy used relied on there being a very high level of sequence conservation in the 5' and 3' untranslated regions that had been indicated in early terminal sequencing and fingerprinting studies (Clarke & McCrae, 1983; Imai et al., 1983
; McCrae & McCorquodale, 1983
). The validity of this strategy was confirmed by the fact that, in all cases, a major PCR amplicon of the expected size was obtained. The mammalian isolates examined in general had VP3-encoding genes of 2591 nt with 5' and 3' untranslated regions of 49 and 34 nt, respectively. The two exceptions to this rule were the bovine isolates; the RF isolate has one additional base in the 5' untranslated sequence and the gene from the UKtc isolate is one base shorter in the 3' untranslated region. The mammalian isolate genes all had a single long open reading frame (ORF) encoding a protein of 835 aa with a predicted molecular mass of
98 kDa. This molecular mass estimate is consistent with that observed for VP3 by PAGE analysis, and supports the conclusion reached in the earlier protein analyses that this protein is not subjected to any major post-translational modifications in virus-infected cells. The VP3 gene of the avian virus isolate, CH-2, was found to be shorter than the mammalian isolates, at 2583 nt, with an ORF capable of encoding a VP3 protein of 829 aa, which nevertheless still had a predicted molecular mass of
98 kDa. This is in broad agreement with the observed molecular mass (Kang et al., 1987
). The 5' and 3' untranslated regions of the avian isolate gene were 49 and 44 nt, respectively (the terminal 25 and 26 bases, respectively, resulted from the PCR primers).
Alignment of the amino acid sequences deduced from the corresponding nucleotide sequences allowed estimates to be made of the extent of amino acid identity between the various isolates examined including those already lodged in GenBank. The results (Table 1) revealed two levels of overall amino acid identity; for virus isolates originating from humans, sequence identity was greater than 94 % with the exception of DS1 and 69M (see later). By contrast, isolates originating from other species showed sequence identities as low as 79 % when compared with human isolates. Interestingly, these values are similar in magnitude to those seen for the external shell glycoprotein VP7 when virus isolates from the same and different G-serotypes are compared. There were two exceptions to these levels of amino acid identity. First, the level of divergence at
45 % (CH-2 versus PO-13, 95 %; CH-2 versus KU, 56 %; and PO-13 versus KU, 56 %) between virus isolates of mammalian and avian origin was much greater than that between isolates made from different mammalian hosts (Table 1
). Secondly, the two human virus isolates DS1 and 69M showed much closer sequence identity to viruses of bovine origin than to other human virus isolates (Table 1
). This suggests that these two human virus isolates may in fact be recent genetic reassortants carrying some genes originating from the human virus isolates but with their VP3 genes having been acquired from a bovine virus through reassortment. In the case of the 69M isolate, previous studies (Qian & Green, 1991
) on the gene encoding the external shell protein VP4 of this isolate revealed that it had a higher level of sequence similarity to virus isolates of animal origin than those coming from humans. By contrast, the NSP1 gene sequences of DS1 and 69M are clearly not of animal origin (Hua et al., 1993
; Xu et al., 1994
). Phylogenetic tree analysis of the data (Fig. 1
) reiterates the interpretation that can be made from the simple percentage identity figures. The higher levels of sequence identity seen in virus isolates from the same host species may indicate that the selective pressure for evolutionary change in VP3 is for the optimization of its interaction with species-specific host proteins. At the individual amino acid level, proline residues at 15 positions (P120, P211, P223, P289, P309, P313, P392, P401, P426, P502, P652, P697, P709, P717, P796) and cysteine residues at four positions (C144, C380, C381, C659) were completely conserved, suggesting that they may have structural importance.
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ACKNOWLEDGEMENTS |
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Received 4 September 2003;
accepted 23 December 2003.
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