Moredun Research Institute, Bush Loan, Edinburgh EH26 0PZ, UK1
Departmento de Sanidad Animal, NEIKER, AB, 48160-Derio, Spain2
Departmento de Patología Animal, Facultad de Veterinaria, 50013-Zaragoza, Spain3
Author for correspondence: Lorenzo González. Present address: Veterinary Laboratories Agency (VLA-Lasswade), Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, UK. Fax +44 131 44 56 166. e-mail L.Gonzalez{at}vla.maff.gsi.gov.uk
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Abstract |
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Using RTPCR, Palmarini et al. (1996) demonstrated the dissemination of JSRV in tissues of the lymphoreticular system (LRS) and in blood mononuclear cells of terminally ill sheep affected with both natural and experimental SPA. Improved PCR techniques have allowed the detection of JSRV proviral DNA in LRS tissues and have shown that viraemia can precede SPA tumour development in experimentally infected sheep (Holland et al., 1999
; García-Goti et al., 2000
). In this paper, we describe the detection of JSRV proviral DNA in the blood and LRS tissues of clinically affected and unaffected sheep with pathological evidence of natural SPA and in the same range of samples of SPA in-contact animals (non-affected sheep from SPA-affected flocks) with no evidence of pulmonary neoplasia.
A total of 36 Latxa ewes from commercial flocks was selected as part of a wider study into chronic respiratory diseases (CRD). These sheep were classified into four groups according to their flock of origin, clinical status and gross and histopathological pulmonary lesions. These latter features have been described in detail elsewhere (González, 1990 ; García-Goti et al., 2000
). Sheep were grouped into: (i) classical SPA (cSPA; ten sheep with clinical evidence of CRD, all with large, diffuse and fluid-productive adenocarcinomas), (ii) atypical SPA (aSPA; four clinically normal sheep and two CRD-affected sheep with advanced lesions of maedi; all six had small solitary or multifocal, white, dry, circumscribed lung tumour nodules), (iii) in-contact (three clinically normal sheep and seven sheep affected by maedi; none of them had gross or histological evidence of neoplastic lung lesions), and (iv) controls (ten clinically normal sheep, with no gross or histological evidence of pulmonary lesions of either SPA or maedi).
The 26 sheep in the first three groups came from five different flocks with a history of SPA. The ten sheep in the control group came from two flocks in which no clinical or pathological evidence of SPA had been found in the preceding 16 years. The mean ages of the sheep in each of the groups were 5·6, 5·3, 6·9 and 8·0 years, respectively.
Before necropsy, peripheral blood was collected into EDTA tubes and peripheral blood leukocytes (PBLs) were isolated by centrifugation after lysis of erythrocytes (García-Goti, 1999 ); the resulting cell pellets were stored at -80 °C. Sheep were then humanely culled and examined macroscopically. Tissues for PCR examination were taken with separate sterile instruments in the following order: brain, kidney, mammary gland, mammary lymph node, spleen, mediastinal lymph node, non-neoplastic areas of the lung and, finally, neoplastic areas (if present). Tissue samples were placed into individual vials, immediately frozen in liquid nitrogen and stored at -80 °C. Samples of the adjacent areas of the same tissues were also taken and placed in 10% buffered formalin for subsequent histopathological examination, according to routine procedures.
A hemi-nested PCR that was specific for the U3 region of exogenous JSRV (U3-hn-PCR) was used to detect JSRV proviral DNA in the blood and tissue samples, as described previously (Palmarini et al., 1996 ; García-Goti et al., 2000
). Briefly, tissue samples and PBL pellets were thawed and homogenized. Homogonates were then resuspended in EDTA and TrisHCl (pH 8·0), incubated with proteinase K and boiled. Total DNA was extracted by a modified phenolchloroform method (Sambrook et al., 1989
). Primers PI and PIII were used in the first round of PCR amplification and primers PI and PVI were used in the second round to amplify a 133 bp sequence. PCR products were visualized following electrophoresis in agarose gels stained with ethidium bromide. U3-hn-PCR detects a single copy of JSRV provirus in a background of 500 ng of normal sheep DNA (Palmarini et al., 1996
). In the present study, sixx300 ng replicates of each DNA sample (a total of 1·8 µg) were tested in order to increase the sensitivity of the PCR procedure and to quantify the results.
The number of positive PCR replicates obtained from the six tested was compared using the InStat statistics package (GraphPad Software). Due to constraints in the SD in the different groups (Bartlett test) and in the normality of the data (KolmogorovSmirnov test), a non-parametric analysis of variance (KruskalWallis test and Dunns Multiple Comparisons test) was used to compare the median PCR values of the different groups. Two comparisons were performed, one between groups of sheep (as defined above) for the same type of sample and the other between the different tissue samples within each group of sheep. PCR results of kidney, brain and mammary gland were all grouped as extra-thoracic non-lymphoid (ENL) organs.
The results of JSRV proviral DNA detection in the blood and tissue samples of sheep from the four different groups are detailed in Table 1. Overall, JSRV was detected in all 16 SPA sheep (classical and atypical) and in 8/10 SPA in-contact sheep. None of the 71 samples tested from the 10 control sheep was positive following U3-hn-PCR. JSRV was detected in PBLs, lung tumour, mediastinal lymph node and non-neoplastic lung from all cSPA sheep and in a lower proportion of the spleen and mammary lymph node samples. Proviral DNA was detected in all lung tumour samples from aSPA sheep, in all but one of the PBL samples, mediastinal lymph nodes and non-neoplastic lungs, and in a lower proportion of spleen and mammary lymph node samples. JSRV was detected in 40% of PBLs of SPA in-contact sheep and in a lower proportion of the mediastinal lymph node, spleen, mammary lymph node and non-neoplastic lung samples.
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Previous studies have demonstrated the presence of JSRV in the blood of sheep with experimentally induced and natural SPA (Palmarini et al., 1996 ; Holland et al., 1999
). The present report clearly shows that JSRV can be detected in naturally infected live sheep before the onset of clinical SPA and even in the absence of discernible lung tumours. Although only a small number of in-contact sheep were examined, it seems clear that subclinical JSRV infection can reach a high prevalence in flocks affected with SPA.
In agreement with previous reports (Palmarini et al., 1996 ; Holland et al., 1999
), dissemination of JSRV infection outside the lung appears to be restricted to LRS organs and PBLs, whereas non-lymphoid organs remain mostly negative. Within the LRS, the mediastinal lymph node presents the highest proportion of JSRV infection in animals with lung tumours, but not in in-contact sheep. This may be due to the role of this lymph node in the local drainage from the lung and to the presence of tumour metastases (García-Goti et al., 2000
). The detection of JSRV in the blood also deserves attention, as this sample shows the largest number of positive results among those samples not related to the respiratory tract and, in fact, the largest overall in the in-contact group of sheep. Further studies are needed to clarify whether LRS infection is a necessary phase in SPA, similar to that for mouse mammary tumours of retroviral aetiology (Callahan & Smith, 2000
), and whether it compromises the host immune response.
Overall, the neoplastic lung is the tissue that gives the largest proportion of positive PCR results, both in terms of sheep and replicates, a finding that is in agreement with previous reports on JSRV detection by immunohistochemistry or blocking ELISA techniques (Palmarini et al., 1995 ). Our results also indicate that LRS dissemination of JSRV is pronounced in animals with large, fluid-producing tumours (cSPA), intermediate in sheep with small, circumscribed, dry tumour nodules (aSPA) and low in in-contact unaffected sheep. Statistical analyses were not, in all cases, conclusive in this respect, but this might be a consequence of the use of non-parametric tests, which, although the most appropriate for our data, may lack the power to identify real differences between groups. Bearing this in mind, we hypothesize that JSRV dissemination is very low during the pre-clinical stages of infection and increases following the initiation and development of neoplastic lesions due to active virus replication in the tumour cells. However, the precise factors that regulate the genesis and progression of the tumour remain to be elucidated.
With regard to the detection of JSRV proviral DNA in the blood of clinically affected sheep, the performance of the PCR protocol used in this study (10/10 positive sheep with 2/6 to 6/6 positive replicates) is better than that previously reported by Palmarini et al. (1996) (0/6 sheep) and Holland et al. (1999)
(5/7 sheep with 1/8 to 2/8 positive replicates). However, the potential for its use in the detection of subclinical infection in live animals seems to be compromised by the low levels of infected blood cells at this stage. In our study, JSRV was detected in the PBLs of only 4/8 positive in-contact sheep and three of them gave only one positive PCR replicate. These figures are in agreement with the estimated low frequency of JSRV infection of blood monocular cells in clinically affected sheep (less than 1/240000; Holland et al., 1999
) and with the fluctuating positive PCR results obtained in a prospective field study on JSRV viraemia in clinically normal sheep from an SPA-affected flock (García-Goti, 1999
). Nevertheless, the development of a more sensitive, high-throughput PCR test that would allow repeated testing would greatly facilitate the realization of longitudinal surveys in affected flocks and permit the identification of routes and risk factors of transmission of JSRV infection.
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References |
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Received 10 November 2000;
accepted 23 February 2001.