MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK1
US Military HIV Research Program, Rockville, MD, USA2
University of Reading, School of Animal & Microbial Sciences, PO Box 228, Reading, UK3
Author for correspondence: Arvind Patel. Fax +44 141 337 2236. e-mail a.patel{at}vir.gla.ac.uk
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Abstract |
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The mechanism(s) of HCV entry into its target cell is currently unknown, although the E2 glycoprotein is thought to play a major role in viruscell attachment (Rosa et al., 1996 ). The E2 glycoprotein extends from aa 384 to 746 of the polyprotein and it carries regions of extreme variability (Kato et al., 1992
; Mizushima et al., 1994
; Ogata et al., 1991
; Weiner et al., 1991
). The most variable region (HVR-1) is located within the N-terminal 27 residues (aa 384411) of E2, and a second region, HVR-2, resides in the 476482 segment. Antibodies specific for epitopes within HVR-1 have been reported to inhibit the binding of E2 glycoprotein to cells and to block HCV infectivity in vitro and in vivo (Farci et al., 1996
; Habersetzer et al., 1998
; Rosa et al., 1996
; Shimizu et al., 1996
; Zibert et al., 1995
). Because of the lack of a suitable cell culture system for in vitro propagation of HCV and the unavailability of virions in sufficient quantities, it has not yet been possible to assess the precise role of the glycoproteins in cell attachment and entry. Instead truncated, secreted versions of E2 have been used as soluble mimics of the native forms present on virus particles. Pileri et al. (1998)
reported that both a truncated soluble form of E2, and HCV virions bound to the large extracellular loop (LEL) of the cell surface protein CD81, suggesting a potential receptor candidate for HCV. The interaction between CD81 and truncated forms of E2 or the E1E2 heterodimer was confirmed and further analysed by several groups (Chan-Fook et al., 2000
; Flint et al., 1999
, 2000
; Hadlock et al., 2000
; Meola et al., 2000
; Patel et al., 2000
; Petracca et al., 2000
; Wunschmann et al., 2000
). However, a comparative antigenic characterization of the various forms of HCV glycoproteins (including VLPs) binding to CD81 has not been reported. From the point of view of structurefunction analysis, it is important to consider whether antigenic and/or structural differences exist between soluble and native forms of glycoproteins and to ensure that appropriate ligands are selected carefully to mimic accurately the native viral forms. In contrast to the soluble or non-particulate forms of HCV structural proteins, those present in VLPs may be more likely to represent the native, virion-like conformation(s).
In this study, we investigated the CD81-binding characteristics of various forms of HCV glycoproteins. Recombinant vaccinia virus (v1-836) or baculovirus (rbac-B45) expressing aa 1836, representing the entire structural region (core, E1 and E2) plus p7 and the N-terminal 27 aa of NS2 of the infectious clone of HCV genotype 1a strain H77c (Yanagi et al., 1997 ) were generated. Full-length (FL) E1E2 complex was produced by infecting COS-7 cells with v1-836 for 24 h; the cells were then washed in PBS, lysed in lysis buffer (20 mM TrisHCl, pH 7·4; 150 mM NaCl; 1 mM EDTA; 0·5% Triton X-100), and the lysate used as a source of FL E1E2 for assays described in this study. The soluble form of E2, E2660, was produced in COS-7 cells using a recombinant vaccinia virus (vE2660) described previously (Patel et al., 2000
). The medium of COS-7 cells infected with vE2660 was used as a source of E2660. Expression of HCV structural proteins in mammalian or insect cells infected with the appropriate recombinant virus was confirmed by immunofluorescence, Western blot and immunoprecipitation assays (data not shown).
Insect cells [Spodoptera frugiperda (Sf)] were infected with rbac-B45 to produce HCV VLPs, which were partially purified by sucrose gradient centrifugation essentially as described by Baumert et al. (1998). Electron microscopic (EM) analysis of VLPs revealed a population of particles with radii ranging from 20 to 44 nm, the morphology of which was consistent with that predicted by analogy to related pesti- and flaviviruses. Particles were at least partially enveloped with spikes protruding from the surface (Fig. 1A). The presence of HCV E2 glycoprotein on the surface of VLPs was confirmed by immunogold electron microscopy (Fig. 1A
, inset). Similarly, the E1 glycoprotein was also shown to be present on the surface of VLPs (data not shown). A detailed structural and antigenic characterization of the VLPs will be described elsewhere.
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We next evaluated a panel of well-characterized mouse and rat monoclonal antibodies (MAbs) specific for epitopes in E2 [Table 1; Flint et al. (1999)
; A. H. Patel and others, unpublished] for their ability to inhibit the interaction with human CD81 of the various HCV glycoprotein ligands. E2660, FL E1E2 complex or VLPs were pre-incubated with various MAbs and ligandMAb complex formation was demonstrated in a GNA lectin-capture assay followed by detection with an anti-species IgGhorseradish peroxidase (HRP) conjugate (data not shown). The ligandMAb complex was allowed to bind GSThCD81 in the ELISA capture assay described above and the bound proteins were detected using the anti-E2 polyclonal serum R646 followed by anti-rabbit IgGHRP conjugate. Several MAbs were able to block binding of the glycoproteins to CD81, albeit with varying efficiencies (Fig. 2
). MAbs which showed 80% or greater inhibition of binding are used here to highlight the differences in CD81 binding characteristics of various ligands. MAbs AP33 and 3/11 (specific for aa 412423, Fig. 2
; Table 1
) inhibited the interaction of all three ligands with CD81. MAbs 9/27 (HVR1, aa 396407) and 9/75 (aa 528535) inhibited the interaction of FL E1E2 or VLP with CD81, and had modest affects on the E2660CD81 interaction (Fig. 2A
C
, Table 1
). In addition, MAbs 11/20c, 7/16b (both recognizing aa 436447 of E2), 2/69a (aa 432443), and 1/39 (aa 436443) all efficiently blocked the interaction of VLPs (Fig. 2C
), but not E2660 or FL E1E2 complex (Fig. 2A
, B
), with CD81 (Fig. 2A
C
). The differential inhibition of CD81 binding is not due to differences in MAb reactivity with the various antigens, since all of the above MAbs recognized the E2 ligands comparably (data not shown). Taken together, these results indicate structural differences between different E2 glycoprotein forms.
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The inhibitory activity of MAbs could be due to occupancy of epitopes involved in direct contact with CD81 or may simply be a consequence of occlusion by steric hindrance. In addition to highlighting the presence of structural differences between different forms of viral glycoproteins, our results have broadly identified three regions (namely, HVR-1, aa 412447 and 528535) within E2 that may modulate E2CD81 interaction. Of the five anti-HVR1 MAbs, only MAb 9/27 blocked the interaction of FL E1E2 and VLPs (and to a lesser extent E2660) with CD81, indicating that HVR1 is probably not involved in direct contact with CD81. This is consistent with our recent data which showed that chimeric E2 glycoproteins, and those lacking HVR1, bind CD81 with an affinity comparable to the parental glycoprotein (Patel et al., 2000 ; Forns et al., 2000
). Several reports have implicated HVR-1 as having a role in eliciting neutralizing antibodies and as a region responsible for interacting with the cell surface (Farci et al., 1996
; Rosa et al., 1996
; Shimizu et al., 1996
; Zibert et al., 1995
). Given that CD81 is expressed in a wide range of cell types (Levy et al., 1998
), it is unlikely to be a determinant of hepatotropism for HCV. Our unpublished results, along with results from others (Agnello et al., 1999
; Petracca et al., 2000
), suggest that other candidate receptor(s) for HCV exist, and that involvement of HVR-1 in E2 binding the cell surface via such factors cannot be ruled out.
The epitopes recognized by six of the eight inhibitory MAbs are located within the E2 region 412447 (shaded area, Table 1), indicating that residues in this 35 aa region may play a crucial role in interaction with CD81. Two of the MAbs, AP33 and 3/11, inhibited the interaction of all three ligands with CD81, indicating that the aa residues 412423 may directly contact CD81. The remaining four MAbs (recognizing epitopes located between E2 aa 432447, Table 1
) only block the VLPCD81 interaction (but not E2660 or FL E1E2), indicating that the inhibition may be due to steric hindrance. These data are in keeping with our recent studies showing that the aa residues in E2 region 407524 are important for modulating CD81 interaction (Patel et al., 2000
). In addition to the 35 aa E2 region described above, MAb 9/75 (recognizing aa 528535) also blocked the interaction of all three ligands with CD81, implicating this region as a possible second binding site. Interestingly, using fold recognition methods Yagnik et al. (2000)
recently produced a model of HCV E2 glycoprotein in which they identified aa 474494 and 522551 as CD81-binding regions. Our experimental observations do not entirely support this interpretation in that they implicate the aa region upstream of the first CD81-binding domain 1 depicted in the model. However, the inhibitory activity of MAb 9/75 is consistent with the involvement of the second CD81-binding region proposed by Yagnik et al. (2000)
.
In conclusion, the ligand-dependent differential MAb inhibition of E2CD81 interaction strongly points to differences in the E2 tertiary structure between E2660, FL E1E2 and VLPs. It is important to take this into consideration when a particular viral ligand is selected for structurefunction analysis.
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Acknowledgments |
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Footnotes |
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References |
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Received 28 March 2001;
accepted 25 April 2001.