Institute of Molecular Biology and Genetics, Building 105, Seoul National University, Kwan-Ak-Gu, Seoul 151-742, Korea1
Author for correspondence: Sunyoung Kim.Fax +82 2 875 0907. e-mail sunyoung{at}plaza.snu.ac.kr
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Abstract |
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The NF-B family normally regulates the expression of genes involved in T cell activation and proliferation, such as interleukin 2 (IL-2) and the alpha subunit of the IL-2 receptor (IL-2R) (Greene, 1991
). NF-
B is thought to play an important role in HIV- 1 replication because the HIV-1 promoter contains two NF-
B binding sites (Nabel & Baltimore, 1987
). It has been suggested that the Nef protein influences the activity of NF-
B. However, the nature of the interaction between these proteins appears to be controversial. It has been reported that the Nef protein may inhibit the induction of HIV-1 and IL-2-directed gene expression through interference with the NF-
B activation that occurs upon T cell stimulation (Bandres & Ratner, 1994
; Niederman et al., 1992
) and that the addition of recombinant Nef protein to peripheral blood mononuclear cells decreases T cell surface IL-2R alpha-chain expression (Greenway et al., 1995
). Furthermore, it has been suggested that induction of AP-1 is inhibited in the presence of the Nef protein (Bandres & Ratner, 1994
).
However, a positive role(s) for the Nef protein in T cell activation and HIV gene expression has also been proposed. The secretion of IL-2 and surface expression of IL-2R in response to phorbol 12-myristate 13- acetate (PMA) in Jurkat cells (Schwartz et al., 1992 ) and antigen-dependent T cells (Brigino et al., 1997
) are unaffected by the presence of the Nef protein. Moreover, nef-containing SIV preferentially stimulates the production of IL-2 from 221 cells (Alexander et al., 1997
). These cells are a T lymphoid cell line derived from a rhesus monkey infected with herpesvirus saimiri and are known to closely resemble normal lymphoid cells in that they produce IL-2 only upon stimulation. The positive effect of the Nef protein on HIV-1 replication is most evident when resting CD4+ T lymphocytes are infected by HIV-1 followed by stimulation with T cell mitogens such as phytohaemagglutinin (PHA) and PMA (Miller et al., 1994
; Spina et al., 1994
). Because of these conflicting reports, we initiated a series of experiments designed to help understand the role(s) of nef in viral gene expression. We found, reproducibly, that the Nef protein had little effect on the activity of the cellular transcription factors NF-
B and AP-1.
To study the effects of the Nef protein on the cellular transcription factors NF-B and AP-1, we constructed cell lines that constitutively expressed the Nef protein using a retroviral vector. The nef coding sequence was isolated from the HIV-1 clone pNL432 by PCR, confirmed by nucleotide sequencing after cloning into the retroviral vector MOIN (Kim et al., 1998
), resulting in the construct MOIN-432 nef. In this construct, the nef and neo genes are expressed as a bicistronic message. As a negative control, we used an identical nef sequence, but containing a frameshift mutation at an internal Xho I site. MOIN-432 nef was transfected into 293T cells, together with the amphotropic retroviral packaging construct, pkat 2ampac (Finer et al., 1994
). Cell culture supernatant was taken 2 days after transfection and filtered through a 0·45 µm filter. The human T lymphoid line Jurkat was transduced with cell-free retroviral supernatant and selected in the presence of 1 mg/ml G418 as described by Byun et al. (1996)
. Cell populations were obtained and subjected to limiting dilution for subcloning. A large number of subclones was obtained, but in this paper, we concentrated on the drug-resistant populations (J-432 nef and J-435 nef for nef+ and nef-, respectively) and a few representative subclones (J2-1 to J2-8 for nef+, J5-1 to J5-5 for nef-).
We first tested whether the Nef protein was expressed in these drug- resistant cells. The nef+ cell population (J-432 nef) and all subclones (J2-1 to J2-8) derived from this population expressed readily detectable amounts of the 27 kDa Nef protein (Fig. 1 a; filled-in arrow). The 25 kDa Nef protein (open arrow) was also detected in some lines. It has been suggested that the 25 kDa Nef protein is the translation product of a reading frame initiating at an internal ATG codon in the nef ORF, 57 bases downstream from the first ATG codon (Kaminchik et al.,
). To be certain that nef sequences in these G418-resistant cells were still identical to that of NL432, nef sequences were cloned by genomic PCR from J2-1, J2- 5 and J2-8, followed by nucleotide sequencing. Two sequences were cloned from each line. The nef sequences in these cell lines were all identical to that of the NL432 nef.
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Because the Nef protein was reported to have a negative effect on AP- 1 induction as well as NF-B (Bandres & Ratner, 1994
), we also tested the effect of the Nef protein on induction of AP-1 by the same stimulators using the reporter plasmid AP-1 CAT. This contains a CAT gene under the control of a truncated c-fos promoter with five copies of a synthetic AP-1 binding site (Robbins et al., 1990
). As in the case of NF-
B, there was no significant difference in the level of induced AP-1 activity between the nef+ and nef- populations and subclones (Fig. 1c
). However, the J2-1, J2-5 and J2-8 subclones which showed the highest induced levels of NF-
B also produced the highest levels of CAT activity following stimulation from the AP-1 reporter plasmid.
To test whether the transcriptional activity measured by the CAT assay correlated with DNA binding activity by these transcription factors, nuclear extracts, either prior to or following stimulation with PHA and PMA for 4 h (Niederman et al., 1992 ), were prepared as described previously (Dignam et al., 1983
). They were then incubated with a double-stranded 32 P-labelled NF-
B oligonucleotide probe, the sequence of which was taken from the
B sites of the HIV-1 LTR (-99 to -76) (Kim et al., 1996
), or an AP-1 oligonucleotide probe (Kekule et al., 1993
). The non-inducible transcription factor Oct-1 was used as a control for the integrity of extracts. After incubation for 20 min at room temperature, the reactions were analysed on a 4% polyacrylamide gel. The specificity of the retarded complexes was confirmed by competition with a 50-fold excess of cold wild-type (Fig. 2a
, b
, c
, lane 10) or mutant oligonucleotide (lane 11). Consistent with the CAT assay data, NF-
B induction by PHA and PMA was higher in the J2-1 subclone (Fig. 2 a
, lane 3) than in the J2-3 (lane 5), J5-3 (lane 7) and J5- 4 (lane 9) subclones. The results using the AP-1 probe paralleled those obtained with the NF-
B oligonucleotide (Fig. 2b
), though induction of AP-1 was less prominent than that of NF-
B in J2-1 cells. These data indicate that the induction of DNA binding activity of NF-
B and AP-1 correlates well with the increased CAT activity from the reporter plasmids.
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The results presented here are in agreement with observations that nef expression in antigen-dependent T cell clones does not affect their proliferative responses to antigen, and other parameters of T cell activation, such as the induction of IL-2R alpha-chain expression and cytokine production (Page et al., 1997 ). Because we used the same nef sequence (NL432) and the human T cell line (Jurkat) that had been previously used by other investigators, we do not believe that the use of different experimental tools can account for these observed discrepancies. This is especially so because in all of our nef+ cell lines, we always observed a significant reduction in the level of surface CD4. Because the interaction between the Nef protein and NF-
B has been proposed to play such a key role in virus replication and pathogenesis, it is vital to clarify the relationship between these two proteins.
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Acknowledgments |
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References |
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Received 12 May 1999;
accepted 11 August 1999.