Department of Epidemiology and Public Health, Yale University School of Medicine, 60 College Street, New Haven, CT 06520, USA
Correspondence
Louis Alexander
louis.alexander{at}yale.edu
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ABSTRACT |
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MAIN TEXT |
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HIV-1 and SIV Vif have limited homology and demonstrate species-specific activity (Simon et al., 1998). It has been shown that in human cells, Vif from non-primate lentiviruses, i.e. visna virus, bovine immunodeficiency virus or feline immunodeficiency virus, did not restore efficient virus replication. Conversely, Vif from SIVmac239 did restore efficient replication of Vif-defective HIV-1 (Simon et al., 1995
, 1998
). However, the capacity of HIV-1 Vif to support Vif-defective SIV replication in rhesus monkey cells has not been tested. In this paper, we present data addressing this capacity.
In an attempt to determine whether HIV-1 Vif would function in an SIV background, we engineered a recombinant in which HIV-1 vif sequences replaced SIV vif sequences. Previously, in the construction of SHIV recombinants, HIV-1 sequences have been inserted into the locus normally occupied by analogous SIV sequences (Alexander et al., 1999a; Karlsson et al., 1997
; Kirchhoff et al., 1999
; Reimann et al., 1996a
, b
; Soderberg et al., 2002
; Uberla et al., 1995
). Following these examples, we engineered a recombinant (SHIV/Vif) in which HIV-1 vif sequences were inserted into the vif locus of SIVmac239 (Regier & Desrosiers, 1990
). In the construction of SHIV/Vif, NheI and SacII restriction sites were introduced into the SIV
Vif genome (containing a large deletion in SIV vif) (Gibbs et al., 1994
) using the QuickChange site-directed mutagenesis kit (Stratagene). To facilitate insertion of the HIV-1 vif sequences, NheI and SacII restriction sites were introduced at the 5' and 3' ends, respectively, of HIV-1 vif by PCR. The 5' primer also introduced the consensus translation initiation sequences CCACC immediately 5' of the initiation ATG to optimize Vif protein expression (Kozak, 1981
, 1987
). To ensure that the exact desired sequence was contained in these recombinants, the cloned DNA was subjected to fluorescent sequencing (ABI) using SIV- and HIV-1-specific primers. In order to test the viability of SHIV/Vif, these sequences were fused to 3' SIV sequences and the DNA was introduced into CEMx174Vif cells (which express SIV Vif and can rescue replication of SIVmac239 lacking Vif; Gibbs et al., 1994
) by DEAE-dextran-mediated transfection (Naidu et al., 1988
). The cultures were maintained in RPMI 1640 (Gibco BRL) supplemented with 10 % heat-inactivated foetal bovine serum (FBS; Gibco BRL). The production of replication-competent virus was assessed by the appearance of cytopathic effects and the release of p27Gag into the culture supernatant, which was assayed by ELISA (Coulter). In these cultures, we did not observe the appearance of cytopathic effects (CPE) or the release of p27Gag into the culture supernatant for a period of 8 weeks post-transfection, whereas the parental SIV
Vif replicated similarly to wild-type SIVmac239 in these cultures (data not shown). Furthermore, Western blot analysis using an SIV p17Gag monoclonal antibody (NIH AIDS Research and Reference Reagent Program) did not reveal the production of this structural protein in SHIV/Vif-transfected cells, although it was detected in SIV
Vif-transfected cells (data not shown). These data suggest that the insertion of HIV-1 vif into the SIV vif locus disrupted an unknown element that was essential for SHIV/Vif replication.
We have previously shown that the nef locus of SIV is amenable to the insertion of foreign gene sequences without substantial inhibition of virus replication (Alexander et al., 1997, 1999a
, b
). We tested whether the insertion of HIV-1 vif into the nef locus would result in a replication competent SHIV (SHIV/NVif; Fig. 1
). To facilitate insertion of the HIV-1 vif sequences into the previously described vector SIV
Nef XES (Alexander et al., 1997
), XbaI and SacII restriction sites were introduced at the 5' and 3' ends, respectively, of HIV-1 vif by PCR. As with SHIV/Vif, the 5' primer also introduced the consensus translation initiation sequences to optimize Vif protein expression and the resultant cloned DNA was subjected to fluorescent sequencing. To test the viability of SHIV/NVif, 3' SHIV/NVif sequences were fused to 5' SIV
Vif sequences. The DNA was then transfected into CEMx174 cells. 5' Wild-type SIVmac239 or 5' SIV
Vif were also fused to SIV
Nef sequences (Kestler et al., 1991
) (to create SIV
Nef and SIV
Vif
Nef, respectively) prior to transfection and served as controls for these experiments. As an additional control, wild-type SIVmac239 5' and 3' sequences were fused and transfected into CEMx174 cells. In these cultures, SIV
Nef-, SHIV/Nvif- and SIVmac239-transfected cells displayed CPE within the first week of transfection (data not shown) and p27Gag was detected in the culture supernatants soon after (data not shown). Neither sign of virus replication was detected in the SIV
Vif
Nef-transfected cells, although efficient virus production was detected in CEMx174Vif cells transfected with this recombinant DNA (data not shown). These data indicated that, unlike the insertion of HIV-1 vif in the SIV vif locus, the insertion of HIV-1 vif into the SIV nef locus resulted in a viable SHIV/NVif recombinant.
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Since HIV-1 Vif normally interacts with HIV-1 and human cellular partners, we investigated whether HIV-1 vif sequences were altered during the passage of SHIV/NVif in rhesus monkey PBMC to potentially improve interactions with putative SIV or rhesus monkey cellular partners. For these experiments, cellular DNA from the infected cells was isolated (on day 8 post-infection) using a previously described saturated NaCl technique (Alexander et al., 2000). A fragment of SHIV/NVif that contained the HIV-1 vif sequences was amplified using primers that annealed to SIV env and LTR sequences. The HIV-1 vif sequences were then determined by fluorescent sequencing using SIV- and HIV-1-specific primers, which revealed that they remained unchanged through short-term passage in rhesus monkey PBMC (data not shown). These data suggested that HIV-1 Vif interacted efficiently with putative viral and host cofactors.
The expression of HIV-1 Vif in a foreign locus could affect the efficiency of its expression and, hence, the efficiency of SHIV/NVif replication. Therefore, we examined the efficiency of HIV-1 Vif expression from the nef locus of SHIV/NVif to determine whether Vif expression was comparable in SHIV/NVif and wild-type HIV-1. For these experiments, 1x106 CEMx174 cells were infected overnight with SHIV/NVif or HIV-1 diluted to contain 100 ng p27Gag or p24Gag, respectively. After infection, the cells were washed and resuspended in fresh media. On day 4 post-infection, culture supernatants were collected and the amount of p27Gag or p24Gag produced by the two cultures was quantified by ELISA (Coulter), which revealed that the SHIV/NVif-infected cells had produced 47 ng p27Gag and the HIV-1-infected cells had produced 55 ng p24Gag. The cell pellets from these infections were washed and resuspended in PBS (Gibco BRL). The cells were then lysed in SDS sample buffer and the protein concentration from these lysates quantified using Bradford reagent (Bio-Rad), which revealed that the protein concentration of the two lysates was similar (data not shown). Based on the protein concentration of the lysates and the level of virus produced by these cells, we used approximately 10 % more SHIV/NVif than HIV-1 lysate in our analyses of HIV-1 Vif expression. For these experiments, the lysates were probed with polyclonal anti-Vif antibody (NIH AIDS Research & Reference Reagent Program). The blot was then probed with a secondary peroxidase-conjugated anti-rabbit IgG (Amersham Pharmacia) and incubated with chemiluminescence solution (Perkin Elmer Life Sciences). Radiographic film (Eastman Kodak) was exposed to the membrane and developed. These experiments demonstrated that the level of expression of HIV-1 Vif from SHIV/NVif and HIV-1 Vif was comparable (Fig. 3). These data revealed that HIV-1 Vif was not overexpressed from the nef locus in SHIV/NVif and suggest that HIV-1 Vif most likely functions efficiently in rhesus monkey cells resulting in efficient SHIV/NVif replication in these cells (Fig. 2B
).
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Nef and Vif are normally expressed at distinct times in the virus life-cycle (Desrosiers, 2001; Freed & Martin, 2001
). Nef is an early gene product derived from a multiply spliced message, whereas Vif is an intermediate gene product derived from a singly spliced message. Our experiments show that HIV-1 Vif, expressed from the nef locus, efficiently supported recombinant primate lentivirus replication. Therefore, Vif can be expressed as an early protein product without substantially inhibiting virus replication in human or rhesus monkey cells.
In its normal location, approximately one-third of the HIV-1 or SIV Vif open reading frame overlaps with pol (5') and vpr or vpx (3') sequences, respectively (Desrosiers, 2001; Freed & Martin, 2001
). Thus, it would be difficult to mutate these overlapping sequences without altering additional HIV-1 or SIV gene sequences, which could have adverse effects on virus replication in cell culture that are not Vif related. Our data reveal that HIV-1 Vif can be expressed from the nef locus without substantially affecting virus replication, which we suspect would also apply to SIV Vif. This characteristic could facilitate structure/function studies of N- and C-terminal Vif residue sequences in cell culture in the context of full-length HIV-1 or SIV genomes without the potentially confounding effects of mutated overlapping viral open reading frames.
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ACKNOWLEDGEMENTS |
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REFERENCES |
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Received 23 June 2003;
accepted 25 August 2003.
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