From the Department of Cellular and Molecular Physiology, Yale University, New Haven, Connecticut 06510
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ABSTRACT |
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The human and shark Na-K-Cl cotransporters (NKCCs) are 74% identical in amino acid sequence yet they display marked differences in apparent affinities for the ions and bumetanide. In this study, we have used chimeras and point mutations to determine which transmembrane domains (tm's) are responsible for the differences in ion transport and in inhibitor binding kinetics. When expressed in HEK-293 cells, all the mutants carry out bumetanide-sensitive 86Rb influx. The kinetic behavior of these constructs demonstrates that the first seven tm's contain all of the residues conferring affinity differences. In conjunction with our previous finding that tm 2 plays an important role in cation transport, the present observations implicate the fourth and seventh tm helices in anion transport. Thus, it appears that tm's 2, 4, and 7 contain the essential affinity-modifying residues accounting for the human-shark differences with regard to cation and anion transport. Point mutations have narrowed the list of candidates to 13 residues within the three tm's. The affinity for bumetanide was found to be affected by residues in the same tm 2-7 region, and also by residues in tm's 11 and 12. Unlike for the ions, changes in bumetanide affinity were nonlinear and difficult to interpret: the Ki(bumetanide) of a number of the constructs was outside the range of sNKCC1 and hNKCC1 Kis.
Key words: cotransporter; kinetics; binding; mutations; Na-K-Cl ![]() |
INTRODUCTION |
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The Na-K-Cl cotransporter (NKCC; also called BSC)1 is
an integral membrane protein that facilitates the passage of the ions Na, K, and Cl across cell membranes.
The transporter has been identified in a wide variety of
cell types from vertebrate as well as invertebrate species
(Xu et al., 1994; Delpire et al., 1994
; Payne et al., 1995
;
Payne and Forbush, 1994
). NKCC belongs to the cation-chloride cotransporters (CCC; Haas, 1989
; Gillen et al., 1996
), a family that also includes the Na-Cl
cotransporter (NCC or TSC; Gamba et al., 1993
) and
the K-Cl cotransporter (KCC; Gillen et al., 1996
; Payne
et al., 1996
). Two types of Na-K-Cl cotransporters have
been identified. NKCC1 is a widely distributed isoform
(Xu et al., 1994
); it is found in nonpolarized cells
where it plays an important role in cell volume regulation (Haas, 1989
), and it is also prominent in certain
secretory epithelia where it drives net transport of fluid
and electrolytes (Xu et al., 1994
). NKCC2 is a kidney-specific isoform that occurs as three splice variants
called A, B, and F; these variants are distributed along
the thick ascending limb of Henle and play an important role in electrolyte absorption (Payne and Forbush,
1994
; Igarashi et al., 1995
).
All NKCCs described to date are characterized by a
strict dependence of transport on the three ions, Na, K,
and Cl (Forbush and Palfrey, 1983; Lytle et al., 1992
;
Moore et al., 1995
). In most tissues, the binding stoichiometry is 1Na:1K:2Cl per transporter and, therefore,
the transport process is not associated with net movement
of charge (Haas, 1989
). Loop diuretics such as furosemide, bumetanide, and benzmetanide inhibit cotransporter function by interacting with the protein at an extracellular site (Forbush and Palfrey, 1983
; Payne and
Forbush, 1995
; Isenring and Forbush, 1997
). This process also requires the presence of the ions Na, K, and Cl
in the binding media. Inhibition of transport activity by
bumetanide occurs with a stoichiometry of one inhibitor molecule per transporter protein (Forbush and Palfrey, 1983
).
Members of the Na-K-Cl cotransporter family exhibit similar hydropathy profiles, delineating three broad regions: large NH2 and COOH termini, and a 500-residue central domain that is predicted to contain 12 transmembrane domains (tm's). Sites of regulatory phosphorylation have been identified in the NH2 and COOH termini, demonstrating that part of these domains are intracellular. The central domain of the CCC proteins exhibits the highest sequence conservation. For example, the NH2 terminus, the COOH terminus, and the tm's of the shark and human cotransporters are 55, 90, and 85% identical, respectively.
Despite a high level of amino acid sequence conservation in the NKCC1, apparent affinities for the ions
and for bumetanide vary as functions of the species
(Payne and Forbush, 1995; Isenring and Forbush, 1997
;
Isenring et al., 1998b
). For example, the Km for all the
ions and the Ki(bumetanide) are four- to sixfold lower in the
human cotransporter compared with the shark protein.
In previous studies, we took advantage of these differences to identify regions that are responsible for mediating transport and binding characteristics in the two
species. By interchanging the NH2 and COOH termini
between the shark and human carriers, we were able to
demonstrate that the large hydrophobic central domain encodes all of the information involved in specifying the kinetic singularities of the wild-type proteins
(Isenring and Forbush, 1997
). Further experiments
showed that the second tm plays an important role in
the species differences in both cation and bumetanide
affinities (Isenring et al., 1998a
). Interestingly, this domain is not involved in determining Cl affinity, suggesting that residues beyond tm 3 encode cotransporter behavior for anion transport.
In this study, we have pursued the chimera approach and examined the role of tm's 4-12. We find that variant residues between tm's 4 and 7 also account for human-shark differences in ion transport. Further analysis using site-specific mutagenesis confirms the results obtained with the chimeras, and in conjunction with the results described above, shows that tm's 2, 4, and 7 contain the essential residues that determine the kinetic behavior of the NKCC1s. On the other hand, we find that bumetanide binding involves multiple residues localized before as well as beyond tm 7.
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METHODS |
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Nomenclature for cDNA Constructs
As previously described (Isenring and Forbush, 1997), we use single letters in lowercase to designate domains from an NKCC species (h, human; s, shark) that are included in a chimera. A number 1.x-12.x refers to the position of the chimeric junctions in
the 12 putative tm's, and a number 1L.x-11L.x refers to the connecting loops after each tm
helix. Each domain is further divided into 10 subdomains from x.0 to x.9. The application of this
nomenclature is illustrated in Fig. 1, which presents models of
the NKCC1 constructs used in these studies.
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All the point mutations described here are in the sNKCC1 sequence and consist of sNKCC1 hNKCC1 replacements. A number in parenthesis indicates the site of the mutations following
the nomenclature described above. In GD-VL(5.3) for example, the
GD and VL residues from hNKCC1 replace the corresponding VN
and TV residues in the sNKCC1 sequence; the substitutions are
located near the middle of tm 5 (site 5.3). These point mutations
are shown in Fig. 1.
Vectors
A mutant p-Bluescript (pBSM-SK) containing additional restriction sites in the polylinker (Isenring and Forbush, 1997
) was used
to facilitate various subcloning steps. The wild-type sNKCC1 was
subcloned in the vector pTZ18U (Bio-Rad Laboratories, Richmond, CA) at XbaI-KpnI sites to serve as a template for site-directed mutagenesis. The vector used for expression of wild-type and mutant cDNA (pJB20M) was as in Isenring and Forbush (1997)
. The
vector pJB20 is derived from pCB6 and contains additional intronic sequences in the 3' linker to improve transcript stability.
The vector also contains the strong cytomegalovirus promoter
and a G418 resistance cassette for selection of transfectants. In
pJB20M, additional restriction sites were also introduced in the
polylinker to facilitate subcloning.
Chimeras and Mutations
The chimeras hsh5L.7/13.1 and shs5L.7/13.1 (shown in Fig. 1, A
and B, respectively) were generated by fragment exchange at the
restriction site ApaI situated at bp 1596 of hNKCC1. This site occurs at three other locations in the hNKCC1 cDNA (bp 341, 381, and 660), but is absent in sNKCC1. We used the Kunkel method to
introduce an ApaI site (sNKCC1APA/pTZ18U) at the junction 5L.7 in the sNKCC1 cDNA (bp 1785); the mutagenic oligonucleotide,
shown in Table I (A), changes the valine at position 450 in
sNKCC1 to a glycine in the extracellular loop. The junction corresponds to residues V450 G/P451 in sNKCC1 and G478/P479 in
hNKCC1. The other junction site at 13.1 corresponds to residues
H756/V757 in sNKCC1 and H783/V784 in hNKCC1. After creating the
ApaI site in sNKCC1, hsh5L.7/13.1 was generated in two separate
steps. First, the Bst1107I-ApaI fragment of sNKCC1APA/pTZ18U
(600 bp) was ligated to the Bst1107I-ApaI fragment of hNKCC1/
pJB20M (9,600 bp) to generate shs0.9/5L.7 in pTZ18U. In the second step, the following fragments were ligated: KpnI-Bst1107I
from hNKCC1/pJB20M (1,100 bp), Bst1107I-PmlI from shs0.9/
5L.7 in pTZ18U (1,500 bp), and PmlI-KpnI from hNKCC1/
pJB20M (7,800 bp). The chimera shs5L.7/13.1 was generated in a
single step ligation using the following fragments: KpnI-ApaI from
sNKCC1APA/pTZ18U (1,800 bp), ApaI-PmlI from hNKCC1/ pJB20M (900 bp), and PmlI-KpnI from sNKCC1/pJB20M (8,800
bp). Representations of the chimeras are shown in Fig. 1, A and B.
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For the chimeras hsh7.9/13.1 and shs7.4/13.1 (shown in Fig. 1,
C and D, respectively), the junction points are in tm 7 (Y506/A507 V in sNKCC1 and Y534/V535 in hNKCC1) and at 13.1 (as described above). For the tm 7 junction, a SnaBI restriction site is present in
hNKCC1 (bp 1761) but absent in the shark sequence. We used the
Kunkel method to obtain a matching site in sNKCC1 at bp 1950. The SnaBI-containing oligonucleotide, shown in Table I (B), also introduces four substitutions in sNKCC1; the residues I504, A507, A509, and V511 are replaced by their counterparts L532, V535, I537, and
A539 from hNKCC1. The resulting mutant, L-V-I-A(7.7)/pTZ18U or
sNKCC1SNAB/pTZ18U, was used to generate hsh7.9/13.1 and
shs7.4/13.1. The KpnI-SnaBI fragments of hNKCC1/pBSM-SK
(1,800 and 5,300 bp; see Isenring and Forbush, 1997
) and of
sNKCC1SNAB /pTZ18U (2,000 and 6,200 bp) were cross-ligated to
generate hs7.9/pTZ18U and sh7.4/pBSM-SK
; the KpnI-PmlI fragment of each mutant (2,500 and 2,700 bp) was religated to the
KpnI-PmlI fragment of hNKCC1/pJB20M or sNKCC1/pJB20M to
generate hsh7.9/13.1 and shs7.4/13.1. As illustrated in Fig. 1, C
and D, these two chimeras are not perfect complements of one another since they share human residues in tm 7.
The chimera hsh10.5/13.1 (see Fig. 1 E) was produced by exchanging human/shark fragments at NdeI and PmlI restriction
sites after creating a NdeI site in sNKCC1 at bp 2410 (sNKCC1NDE/
pTZ18U) to match the human site at bp 2218 [see oligonucleotide
in Table I (C)]. The junction point 10.5 corresponds to Y686/A687
in hNKCC1 and to Y659/A660 in sNKCC1. Chimeras hs3.1 and sh3.1
were available from the studies in Isenring et al., 1998a.
The point mutations AI-V-I-L(4.5), GD-VL(5.3), and L-V-I-A(7.7), shown in Fig. 1, F-H, were created with the Kunkel method using mutagenic oligonucleotides [see Table I (D-F)]. Residues in sNKCC1 were replaced with corresponding residues from hNKCC1: AI-V-I-L(4.5) was generated by replacing the S386, V387, I389, V391, and F393 residues in the tm 4 sNKCC1 sequence by the A414, I415, V417, I419, and L421 residue counterparts from hNKCC1; for GD-VL(5.3), V419, N420, T422, and V423 in tm 5 were replaced by G447, D448, V450, and L451; for L-V-I-A(7.7), I504, A507, A509, and V511 in tm 7 were replaced by L532, V535, I537, and A539.
Silent restriction sites or residue substitutions were confirmed by partial automated sequencing (W.M. Keck Foundation Biotechnology Resource Lab DNA Sequencing Group, Yale University) and/or by extensive restriction analyses.
Cell Lines
Lines stably expressing hNKCC1and sNKCC1 were the same as in
Isenring and Forbush, 1997. The five chimeras and three point mutations were transfected into HEK-293 cells by calcium phosphate precipitation and stable lines (generally 12-16) were isolated by G-418 resistance after screening with 86Rb influx assays,
Western blotting, or immunofluorescence. Transfected HEK-293
cells were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Life Technologies,
Inc., Grand Island, NY), penicillin, streptomycin, and G-418 (950 µg/ml). The level of functional expression achieved with six of
eight constructs was comparable to the wild-type hNKCC1 and
sNKCC1 (Isenring and Forbush, 1997
), approximately six- to
eightfold above the endogenous cotransport activity of mock-transfected HEK-293 cells (results not shown). The chimeras
shs5L.7/13.1 and shs7.4/13.1 (see Fig. 1, B and D) were found to
transport 86Rb at a lower rate, approximately threefold above the
background. When cotransporter expression was tested by immunofluorescence using J3 and J7 antibodies, predominant cell surface expression as well as perinuclear staining were observed
(Isenring et al. 1998a
).
Protein Analysis
Experiments were performed as previously described (Lytle et al.,
1992). In brief, transfected HEK-293 cells were allowed to grow
to near confluence and solubilized in 3% CHAPS {3-[(3-chiolamido-propyl)dimethylammonio]-1-propanesulfonate} with protease inhibitors. Whole cell lysates were assayed for protein content by the Micro BCA method (Pierce Chemical Co., Rockford,
IL). Western blots (2-5-µg protein samples) were carried out using the sNKCC1 monoclonal antibodies J3 or J7 (Lytle et al.,
1992
). As previously reported (Isenring and Forbush, 1997
), J3
recognizes a segment of the NH2 terminus located between residues 49 and 196, and J7 recognizes a COOH terminal segment
situated between residues 1050 and 1168. As shown in Fig. 2, the
level of cotransporter synthesis and the pattern of glycosylation
were comparable for all the cell lines studied in this project. Recognition by J3 and J7 was also consistent with whether the NH2 or
COOH terminus was derived from the shark NKCC1.
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Flux Studies
As previously described (Isenring and Forbush, 1997), HEK-293
cells were subcultured onto 96-well plates precoated with polylysine and grown to confluence (6-8 d). Ion transport rates and
bumetanide binding were determined by 86Rb influx measurements. All experiments were done at room temperature (
22°C)
and the solutions were at pH 7.4. The cells were preincubated in
hypotonic low Cl (163 mosM, 2 mM Cl) medium for 1 h (67.5 mM Na gluconate, 2.5 mM K gluconate, 0.5 mM CaCl2, 0.5 mM
MgCl2, 0.5 mM Na2HPO4, 0.5 mM Na2SO4, and 7.5 mM Na
HEPES) to activate cotransporter activity. For bumetanide inhibition studies, the cells were also preincubated 15 min with the inhibitor diluted in a 20-mM Cl solution (154 mM Na and 144 mM
Cl). The influxes were performed for 1 min in a medium containing 135 mM NaCl, 5 mM RbCl (2 µCi/ml 86RbCl), 1 mM
CaCl2, 1 mM MgCl2, 1 mM Na2HPO4, 1 mM Na2SO4, 15 mM Na
HEPES, and 0.1 mM ouabain; when necessary, Na and Rb were
replaced with equimolar amounts of N-methyl glucamine, and Cl
was replaced with gluconate. To determine bumetanide-sensitive
influx, 250 µM bumetanide was added directly to the media. 86Rb
uptake was terminated by addition of, and three rinses in, ice-cold stop solution (135 mM K gluconate, 5 mM Na gluconate, 1 mM
CaCl2, 1 mM MgCl2, 1 mM Na2HPO4, 1 mM Na2SO4, 15 mM Na
HEPES, 250 µM bumetanide, and 0.1 mM ouabain). Cells were
solubilized in 2% sodium dodecylsulfate, and assayed for protein
content and for 86Rb by counting Cerenkov radiation.
Each concentration curve was carried out in a single row of the 96-well plate, and in a typical experiment there were two to six replicate rows. Data were handled on a per-row basis and counts were normalized to the value at the highest ion concentration, or to the value of uninhibited flux in inhibition studies. Km and Ki values were obtained by nonlinear least-squares curve fitting using the Simplex algorithm (program PLOT, B. Forbush), and are expressed as means ± SEM among all rows in 4-15 experiments. Rows with obvious rogue values (data sets for which least squares fits did not converge, or for which individual data points were >2.0 or <0.5 of the value of the fit line) were omitted from averages, in all cases less than one row in eight.
Chemicals and Reagents
Where not otherwise noted, general chemicals and reagents were from Sigma Chemical Co. (St. Louis, MO), and reagents for molecular biology and tissue culture were from New England Biolabs, Inc. (Beverly, MA), FMC Bioproducts (Rockland, ME), and GIBCO BRL (Gaithersburg, MD).
Calculation of Kinetic Parameters for the Ordered Binding Model
Predictions of kinetic parameters of the ordered binding model
of Miyamoto et al. (1986) and Lytle et al. (1998)
were obtained by solving the set of simultaneous rate equations describing the transport model (see Benjamin and Johnson, 1997
). Isotopic exchange was included by explicitly considering labeled and unlabeled Rb as independently transported species. Solutions were
obtained at various concentrations of a test ion, and Kms were obtained from fits of these curves (for Cl, a fixed Hill coefficient, n = 2, was used for parallelism to our treatment of data; actually, best
fits are obtained with Hill 7
1,6; as discussed in a footnote in
Isenring and Forbush, 1997
). For simplicity, the constants are
symmetrical across the membrane; constants were chosen to produce Kms near those of hNKCC1. All association rate constants were 107 s
1 M
1, translocation rate constants for unloaded and
loaded carrier were 40,000 and 10,000 s
1, respectively, and affinities (1/Kd) for Na, Rb, and Cl (at each of two sites) were 127, 54, and 6 mM, respectively (program PLOT, B. Forbush).
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RESULTS |
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Role of tm's 10-12 in Ion Transport Compared with That of tm's 1-9
To determine if the last two tm's of NKCC1 contain affinity-specifying residues, we replaced these segments
by the corresponding shark sequence (hsh10.5/13.1;
see Fig. 1 E). Between the species, these last two tm's
differ by six residues, while predicted helix 10 is fully
conserved. The dependence of 86Rb influx on [Na],
[Rb], and [Cl] is illustrated in Fig. 3. The data demonstrate that shark residues in tm's 11 and 12 have no effect on the kinetic behavior of hNKCC1. The Km(Na),
Km(Rb), and Km(Cl) are 14, 1.9, and 34 mM, similar to
those reported for the hNKCC1-transfected cells (15, 1.7, and 31 mM, respectively).
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Role of tm's 7-9 Compared with That of tm's 1-6
To next determine the contribution of domains 7-9 in
ion transport by the Na-K-Cl protein, we examined two
other human-shark chimeras with a junction located in
tm 7 (hsh7.9/13.1 and the nearly complementary shs7.4/
13.1; see Fig. 1, C and D). The Km values derived from
the dependence of 86Rb transport on [Na], [Rb], and
[Cl] are summarized in Fig. 4. They show that the
hsh7.9/13.1 chimera has a phenotype that is indistinguishable from hNKCC1; the Km(Na), Km(Rb), and Km(Cl) are 19, 1.8, and 31 mM, respectively. Thus, it appears
that tm's 8 and 9, as shown above for tm's 10-12, do
not confer differences in ion affinities between human
and shark. These results, in conjunction with previous
observations that the NH2 and COOH termini are devoid of affinity-modifying properties (Isenring and Forbush, 1997), indicate that differences in apparent binding parameters between the two cotransporters are
specified by residues found in the first seven tm's.
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Interestingly, ion constants for the nearly complementary chimera shs7.4/13.1 are all intermediate between human and shark. Since the results described above rule out a role for tm's 8-12, tm 7 could contain all of the variant residues accounting for the behavior of shs7.4/13.1. To test this hypothesis, we characterized the ion dependence of 86Rb influx in L-V-I-A(7.7)-transfected cells. In this sNKCC1 mutant (see Fig. 1 H), all the tm-7 residues were replaced by their human residue counterparts. It is seen in Fig. 4 that the ion constants for L-V-I-A(7.7) are very similar to those of shs7.4/13.1. This result confirms that the behavior of shs7.4/13.1, relative to that of sNKCC1, was mediated through substituted residues in tm 7. The data is also consistent with previous observations in showing that residues in tm's 8-12 do not encode variant transport behaviors.
Role of tm's 4-5
Of tm's 1 to 6, only 2 and 4 or 5 could potentially mediate differences in ion affinities between the human and
shark cotransporters; as shown in Fig. 7 A, tm's 1, 3, and 6 are fully conserved. We have previously determined that tm 2 plays a role in cation transport, but is
not involved in Cl affinity differences (Isenring et al., 1998a). Specifically, the chimera hs3.1 had cation affinities that were intermediate between sNKCC1 and
hNKCC1, and a Cl affinity that was similar to that of
sNKCC1 (results from Isenring et al. [1998a] are reproduced in Fig. 5). Thus, with regard to cations, tm's 2 and 7 could contain all of the information that defines the kinetic behavior of NKCC1: each domain endows
approximately half the affinity difference between the
species. For the anions, on the other hand, tm's 4 or 5 are likely to play an important role given that variant
residues in tm 7, as shown above with L-V-I-A(7.7), account for only part of the differences in Km(Cl) between
sNKCC1 and hNKCC1.
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To test these predictions, we analyzed the role of tm's 4 and 5 in Na transport by using two additional chimeras (hsh5L.7/13.1 and shs5L.7/13.1) with a junction point situated in the connecting loop after the fifth tm helix (see NKCC1 in Fig. 1, A and B). The dependence of 86Rb influx on [Na] translates into similar Kms for both chimeras, as depicted in Fig. 5 A, and these constant values are not significantly different from the Km(Na) previously reported for hs3.1. The results confirm that the differences in Na affinity between shark and human are not specified by residues located in tm's 4 and 5.
Unexpectedly, analysis of the same chimeras demonstrates that tm's 4 or 5 are possibly involved in the Rb affinity difference. The data in Fig. 5 B show that when tm's 2, 4, and 5 are from hNKCC1, and 7 is from sNKCC1 (chimera hsh5L.7/13.1; see Fig. 1 A), the Km(Rb) is intermediate between human and shark, and lower than the constants obtained for L-V-I-A(7.7) and hs3.1. In conjunction with our previous observations, these results indicate that three tm's (2, 4 or 5, and 7) may be involved in determining Rb affinity. The behavior of the complementary chimera shs5L.7/13.1 (see Fig. 1 B) is consistent with expectation in showing a Rb affinity that is intermediate between human and shark (Fig. 5 B), and close to that of L-V-I-A(7.7) (6.3 mM compared with 4.8 mM). This result confirms the role of tm 7 as a Rb affinity-modifying domain, and is concordant with the fact that tm 6 is fully conserved between human and shark.
The kinetic behavior of the 5L.7/13.1 chimeras with regard to the Cl dependence of 86Rb influx is summarized in Fig. 5 C. It is seen that the Km(Cl) for hsh5L.7/ 13.1 is intermediate between human and shark (54 mM, compared with 31 and 107 mM for hNKCC1 and sNKCC1, respectively), and that it is similar to the value obtained for L-V-I-A(7.7). The Km(Cl) for the reverse chimera shs5L.7/13.1 is also similar to that of L-V-I-A(7.7)-transfected cells. These results confirm our hypothesis above that tm's 4 or 5, along with tm 7, must account for differences in anion affinity between the human and shark carriers.
To further determine which of tm's 4 and 5 mediated affinity behaviors with regard to Rb and Cl, we analyzed the point mutation GD-VL(5.3) (see Fig. 1 G), in which residues in the tm 5 shark sequence (n = 4) were substituted by corresponding residues from hNKCC1 (a similar tm 4 shark mutant, AI-V-I-L(4.5), was nonfunctional; see Fig. 1 F ). The ion dependence of 86Rb influx for GD-VL(5.3)-transfected HEK cells (Fig. 5) yields constants for Na, Rb, and Cl that are very similar to sNKCC1: 91, 11, and 108 mM, compared with 109, 12, and 107 mM for the wild-type sNKCC1. This finding suggests that tm 5 does not contain affinity-modifying residues; hence, some residues in tm 4 must account for the higher Rb-Cl affinity obtained for hsh5L.7/13.1 compared with hs3.1.
Regions Involved in Bumetanide Binding
sNKCC1 has an apparent affinity for bumetanide that
is three- to fourfold lower than that of hNKCC1 (Payne
et al., 1995; Isenring and Forbush, 1997
). Our previous
studies have demonstrated that these differences are
encoded by the 500-residue central domain of the protein (Isenring and Forbush, 1997
), and that residues in
or near the second tm appear to be responsible for part
of the effect. To extend this analysis, we measured bumetanide inhibition of 86Rb influx in the five chimeras
discussed above, as summarized in Fig. 6.
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Looking first at the results with hsh7.9/13.1 (see Fig. 1 C), we observe that the bumetanide affinity of this chimera is 1.5-fold lower than that of the parent hNKCC1. This indicates that, unlike the situation for ions, residues beyond the seventh tm do have an effect on bumetanide binding. This fact is also illustrated by the behavior of chimera hsh10.5/13.1, which is 2.5-fold more sensitive to bumetanide than is hNKCC1; this shows that residues in tm 11 or 12 affect bumetanide binding.
Surprisingly, the bumetanide-binding behavior illustrated in Fig. 6 does not fit the principle of additivity that seems to apply to the ion transport properties of the chimeras. For instance, three chimeras exhibit bumetanide affinities that are even higher than that of hNKCC1, and one chimera (hsh5L.7/13.1; Fig. 1 A) exhibits an affinity that is even lower than that of sNKCC1. Although, interestingly, the 5L.7/13.1 chimeras exhibit reciprocal forms of this "misbehavior," we have been unable to develop a simple model to explain the data. One possibility is that conformational interactions between extracellular domains affect the structure of the bumetanide binding site.
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DISCUSSION |
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In this study, we have identified regions in NKCC1 that
confer the differences between shark and human NKCC1
in the kinetics of ion transport. By studying chimeric
constructs of shark and human cotransporters, we have
been able to demonstrate that these differences are
caused by variant residues found exclusively in a region
that includes the first seven tm's (see Fig. 7 A). Using site-directed mutagenesis, we further determined that
the residues involved are localized in the predicted tm
helices, but not in the predicted intracellular or extracellular loops. In the current work, we have identified tm's 4 and 7 as determining part of the differences
in Kms: in conjunction with our previous findings (Isenring and Forbush, 1997
; Isenring et al., 1998a
), the
data demonstrate that residues in tm's 2, 4, and 7 contain all of the information specifying shark-human kinetic differences in ion transport.
A summary of the mutagenic mapping studies is shown in Fig. 7 B. Plotted are approximate percent changes in apparent affinity contributed by each tm, inferred from differences in Km values resulting from substitution of residues in the shark sequence. The current studies show that changes in tm 7 affect the Km for all three ions, and that changes in tm 4 impact mainly Rb and Cl affinities. On the other hand, tm 2 residues selectively affect Kms for cations. Two residues have been implicated in determination of Na affinity, and two other residues in the determination of Rb affinity. To a first approximation, the affinity changes that occur in the three individual tm's are additive in accounting for the full difference between shark and human cotransporter kinetics.
We have used site-specific mutagenesis to localize pairs or groups of residues that account for human- shark differences in ion affinities. We have narrowed the search to 13 candidate residues in three tm's, and it is possible that as few as four of these are important: two in tm 2 and one each in tm's 4 and 7. These residues may be within the translocation pocket, interacting directly with transported ions. A helical-wheel diagram of the three tm's is shown in Fig. 7 C, highlighting the candidate residues in boxes. Each helix is oriented with the most hydrophilic side towards the hypothetical pore, and it is seen that in each of the tm's, at least one to two of the candidate residues are on this face.
Interestingly, in predicted tm's 2, 4, and 7, there is
only one charged residue, an arginine at the extracellular end of tm 4. In the Na-K-Cl transporter in which
both anions and cations are proposed to pass single file
through a translocation pocket (Lytle et al., 1998), it
might be expected that charged groups would pose significant barriers to ion entry into or exit from the
pocket. An alternative possibility is that a significant
amount of ion coordination is through oxygen atoms
in serine and threonine side chains as well as in the
peptide backbone (Kaplan et al., 1994
; Doyle et al.,
1998
). Serine and threonine side chains have been implicated in several ion binding sites both indirectly (Arguello and Lingrel, 1995
) and through crystallographic
analysis (Toney et al., 1995
). Interestingly, the ser/thr
composition of tm's 2 and 8 of NKCC is remarkably
high, particularly near the intracellular end of the predicted helices, suggesting a role in ion coordination (the number of threonine residues in tm 2 is even
higher if the helix is one turn longer at the cytoplasmic
end).
As we have previously discussed (Isenring and Forbush, 1997; Isenring et al., 1998a
), regions that confer
species differences in the kinetics of ion translocation
are possibly not the same residues that are essential for
ion binding. Some of the essential residues must be
fully conserved through evolution and these would not
be identified by a technique aimed at correlating functional differences with structural differences. Nonetheless, in the example recently provided by the structure
of a K channel (Doyle et al., 1998
), residues that affect
K selectivity have been shown to be those that are directly involved in ion coordination. This supports our
hypothesis that the identified NKCC1 residues are
closely involved with the ion binding sites and that some
of them may indeed directly participate in ion binding.
The measurable kinetic behavior of an ion transporter is determined by a number of kinetic constants,
including all of the rate constants for the various steps
in the transport cycle. In the case of a multi-ion cotransporter such as NKCC, this is a particularly complex system in which a change in affinity for one ion may
change the Km ("apparent affinity") for other ions. Fortunately, the way in which these constants are interrelated has been determined experimentally for NKCC1
(Miyamoto et al., 1986; also Isenring, P., S.C. Jacoby,
and B. Forbush, unpublished data, for sNKCC1 and
hNKCC1). These data, as well as transport data for various cis and trans ions (Lytle et al., 1998
), support an ordered binding model for Na-K-Cl transport in which extracellular ions bind in the order Na+-Cl
-K+-Cl
and
are released in the same order on the inside of the
membrane (Miyamoto et al., 1986
; Lytle et al., 1998
).
For the present work, we are able to infer the significance of Km changes based directly on the available kinetic data and from predictions of the ordered model.
Such relationships are illustrated in Fig. 8 by kinetic predictions for a particular set of model parameters
(this is only an example: the quantitative behavior depends on the specific choice of parameters, and there
is currently too little information to determine these
uniquely). The interdependence of kinetic constants is
illustrated by the fact that every Km changes in response
to a change in binding site affinity for any of the ions.
However, as a general rule, the apparent affinity of one
ion in the ordered binding scheme is most affected by
changes in the true affinities of ions that bind subsequently, particularly by the affinity of the ion immediately following.
|
With regard to the effect of variant residues in tm 2, Km(Na) is affected by changes in one pair of residues and
Km(Rb) by another pair; there is, however, a smaller
change in Km(Cl) (Isenring et al., 1998a). These results
are probably attributable to changes in the real affinity
for Na and Rb at the two sites, since no other change in
kinetic constants would result in this behavior in the ordered binding model. This interpretation is consistent with the expectation of the model that, at high Na and
Rb concentrations, changes in either Na or Rb affinity
have smaller effects on Kms for the other ions; they are
also consistent with the experimental observations of
Miyamoto et al. (1988) and with our own observations (Jacoby, S.C., P. Isenring, and B. Forbush, unpublished
data) that changes in [Na]ext or [K]ext have sublinear effects on Km(Na) and Km(K), respectively.
The kinetic changes associated with tm 4 substitutions affect Km(Rb) and Km(Cl), but not Km(Na). This behavior is most consistent with a tm 4-related change in the
true affinity for Cl at the second of two Cl binding sites
(see Fig. 8, middle row, ). Differences in tm 7 affect the
affinity for all three ions: this result could reflect a
change in true affinity at the first Cl binding site, but it
could also be explained by a change in multiple kinetic parameters or in the rate constant for movement of the
loaded carrier (Fig. 8, bottom).
This work has also offered considerable insight into
the regions of NKCC involved in loop-diuretic binding.
(a) Expanding on our earlier report (Isenring et al.,
1998a), we find that the pattern of changes that affect
bumetanide binding is not the same as the pattern of
those changes affecting kinetics of ion translocation. This finding is inconsistent with the hypothesis that the
bumetanide binding site is identical with one of the Cl
binding sites (Haas and McManus, 1983
; Turner and
George, 1988
), but it does not rule out the possibility
that some of the residues involved in bumetanide binding are part of the Cl translocation site. (b) In contrast to our observation that changes in ion translocation involves only the first seven tm's, changes in tm 11 and 12 residues are found to alter the kinetics of bumetanide
inhibition. (c) Many of the chimeras exhibit bumetanide affinities that are outside the range of the parent
NKCC molecules, and we have not found a simple
model that can explain the results. One possible explanation is that the bumetanide binding site spans multiple domains on NKCC and that conformational interactions, possibly within extracellular loops, give rise to
complexities in the behavior of chimeric constructs.
In conclusion, we have found that tm's 2, 4, and 7 contain the residues that confer the differences between shark and human NKCC1 with regard to ion
translocation kinetics, and we propose that these helices form part of the Na-Cl-K-Cl binding pocket. Structural considerations suggest that at least four helices
would be involved in forming a translocation "pore"
(Doyle et al., 1998); other candidate helices for the Na-K-Cl cotransporter include the S/T-rich tm 8 and the
very highly conserved tm's 1 and 6. Parameters affecting bumetanide binding appear to be more complex, indicating that loop-diuretic binding involves interactions with multiple regions of the transporter in the
large central domain.
![]() |
FOOTNOTES |
---|
Address correspondence to Paul Isenring, MD, Research Center l'Hôtel-Dieu de Québec, Nephrology Department, 11, Côte du Palais, Québec (Qué), Canada G1R 2J6. Fax: 418-691-5253; E-mail: paul. isenring{at}crhdq.ulaval.ca
Original version received 20 April 1998 and accepted version received 2 September 1998.
Portions of this work were previously published in abstract form (Isenring, P., and B. Forbush III. 1996. J. Am. Soc. Nephrol. 7:1282).We gratefully acknowledge the technical assistance of Mrs. Grace Jones. We also thank Rachel Behnke, Susan Brill, Deborah Lynn, Iñaki Giménez, and Andreas Flemmer for reading the manuscript.
This study was supported by National Institutes of Health grant DK-47661. Paul Isenring was supported by a Clinician Scientist Fellowship from the Medical Research Council of Canada.
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Abbreviations used in this paper |
---|
NKCC, Na-K-Cl cotransporter; tm, transmembrane domain.
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