Insulin Secretion

A High-affinity Ca2+ Sensor After All?



Sebastian Barg1 and Patrik Rorsman2

1 The Vollum Institute, Oregon Health and Sciences University, Portland, OR 97239
2 Oxford Centre for Diabetes, Endocrinology, and Metabolism, University of Oxford, Oxford OX3 7LJ, UK

Address correspondence to Patrik Rorsman, OCDEM, University of Oxford, Churchill Hospital, Oxford OX3 7LJ, UK. Fax: 44-1865-857-217; email: patrik.rorsman{at}drl.ox.ac.uk


Abbreviations used in this paper: HCSP, highly calcium-sensitive pool; LDCV, large dense core vesicles; RRP, readily releasable pool.

Insulin is secreted from the ß-cells of the pancreatic islets by Ca2+-dependent exocytosis of large dense core vesicles (LDCVs) (Ämmälä et al., 1993Go), a process that is triggered by glucose-induced electrical activity (Henquin and Meissner, 1984Go). In response to a step elevation of glucose, insulin secretion follows a characteristic biphasic time course (Curry et al., 1968Go): an initial transient first phase of secretion, which is completed within 10–15 min, is followed by a slowly developing and sustained second phase. It has been estimated that the first phase of secretion is due to the rapid release of a total of 40–80 LDCVs per ß-cell, after which secretion proceeds at a rate of five vesicles per ß-cell per minute (Rorsman and Renström, 2003Go).

The Ca2+ channel density in ß-cell plasma membranes is very low, only about one-twentieth of that in chromaffin cells (Barg et al., 2001Go). Yet, the ß-cell is capable of remarkably high rates of exocytosis. Capacitance measurements have suggested that secretion transiently may proceed at rates as high as 500 LDCV per second (Barg et al., 2001Go). It therefore has been proposed that the ß-cell exocytosis is efficiently coupled to Ca2+ entry via the assembly of a functional complex consisting of Ca2+ channels and exocytotic proteins (Wiser et al., 1999Go) so that exocytosis is triggered by the large increases in the cytoplasmic Ca2+ concentration ([Ca2+]i) occurring at the inner mouth of the Ca2+ channels (Fig. 1 A). In accordance with such a scenario, the rates of exocytosis that can be elicited by voltage-clamp depolarizations require elevation of [Ca2+]i by several tens of micromolar, as estimated from experiments using photolytic release of caged Ca2+ (Takahashi et al., 1997Go; Barg et al., 2001Go). The latter experiments suggested that exocytosis is sigmoidally related to [Ca2+]i with a Kd of ~20 µM and a Hill coefficient (n) as high as 5. By contrast, measurements of insulin secretion from permeabilized cells have indicated that insulin secretion is activated already at submicromolar [Ca2+]i (Yaseen et al., 1982Go; Wollheim et al., 1987Go; Okazaki et al., 1994Go) and capacitance increases have also been observed at such low Ca2+ concentrations (Proks et al., 1996Go). Indeed, the latter type of measurements indicate that exocytosis at Ca2+ concentrations as low as a few hundred nanomolar proceeds at rates 10,000-fold higher than that expected from the Ca2+ dependence quoted above. To date, there has been no explanation of the widely different Ca2+ dependencies of exocytosis indicated by these two experimental paradigms. Two complementary studies appearing in this issue (Yang and Gillis, 2004Go; Wan et al., 2004Go) may explain this conundrum.



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FIGURE 1. (A and B) Schematic illustrating the presence of two populations of granules in ß-cells with distinct Ca2+ sensitivities. Granules with a low-affinity Ca2+ sensor need to be associated with the voltage-gated Ca2+-channels, whereas those equipped with a high-affinity Ca2+ sensor (HCSP) undergo exocytosis in response to a global elevation of cytoplasmic Ca2+. A and B show the situation under basal conditions and following activation of PKA or PKC, respectively. In A, only granules close to the Ca2+ channels are exposed to sufficiently high Ca2+ concentrations to undergo exocytosis (gray areas). In B, moderate but global increases in cytoplasmic Ca2+ (dashed lines; both by Ca2+ influx through the plasma membrane Ca2+ channels and release from intracellular Ca2+ stores) suffice to trigger exocytosis of HCSP granules, which need not be associated with the Ca2+ channels. In addition to the Ca2+-dependent processes discussed here that are involved in the triggering of exocytosis, Ca2+-dependent steps have also been described for more upstream events such as priming/mobilization (indicated by arrow in B). (C) Exocytosis as a function of cytoplasmic Ca2+ concentration for the low-affinity (dashed line) and high-affinity (HCSP; gray line) processes and the sum of the two (i.e., HCSP plus RRP; black line). Note that HCSP attains it maximum already at Ca2+ concentrations <10 µM. Data are derived from Barg et al. (2001)Go assuming a depolarization lasting 50 ms (corresponding to the ß-cell action potential) and Wan et al. (2004)Go. The sum of the two predicts a gradual stimulation of exocytosis over a wide range of calcium concentrations (0.5–30 µM).

 
Recently, a small pool of LDCVs, capable of release in response to moderate (low micromolar) elevations of [Ca2+]i was documented in two types of endocrine cells: adrenal chromaffin cells (Yang et al., 2002Go) and pituitary gonadotropes (Zhu et al., 2002Go). Exocytosis of these vesicles (termed the highly calcium-sensitive pool [HCSP]) proceeds in parallel with the previously described low-affinity Ca2+-dependent exocytosis. Release of HCSP is rapid (rate constant: 10–100 s–1) and at [Ca2+]i < 10 µM, exocytosis of HCSP is actually faster than the rapid initial components previously described. Whereas this could be indicative of separate pathways, both forms of exocytosis involve SNARE proteins. Importantly, the existence of HCSP is not confined to endocrine cells and it has also been found in the rod photoreceptor synapse, where it was postulated to contribute to the linearity of the responses (Thoreson et al., 2004Go).

Using capacitance measurements, Yang and Gillis (2004)Go and Wan et al. (2004)Go now report the presence of HCSP also in rat insulin-secreting insulinoma and primary ß-cells. They demonstrate that HCSP granules are released by global (i.e., throughout the cytosol) rather than localized increases in [Ca2+]i. This suggests that the vesicles in the HCSP are not located in the immediate vicinity of the Ca2+ channels (Fig. 1 A). One explanation for this phenomenon could be the existence of two types of vesicles in the ß-cell: synaptic-like microvesicles (SLMVs) in addition to the LDCVs (compare Kasai, 1999Go; Braun et al., 2004Go). However, Yang and Gillis rule out this possibility by using carbon fiber amperometry to measure exocytosis. They found an excellent correlation between the HCSP capacitance increases and release of the amine. Because serotonin selectively accumulates in LDCVs (Aspinwall et al., 1999Go), this provides strong evidence that the early component of capacitance increase does in fact reflect exocytosis of LDCVs.

The presence of HCSP in ß-cells explains how insulin secretion can proceed at low Ca2+ concentrations. Why then does secretion in the ß-cell (or indeed any of the cells in which HCSP has been described) require two mechanisms of Ca2+ sensing? It is attractive to propose that this feature provides the ß-cell with a means to linearize its secretory response to the stimulus, in a way reminiscent of the situation in the rod photoreceptor (Thoreson et al., 2004Go). As illustrated schematically in Fig. 1 C, the combination of a size-limited high-affinity (HCSP) and a large low-affinity, readily releasable pool (RRP) component allows the ß-cell to regulate its secretory response over a wide range of Ca2+ concentrations, which in turn is proportional to the stimulus intensity.

Under basal conditions, the HCSP is limited to as few as 10 granules, or about one-tenth of the RRP of granules. It is essential that the HCSP is of limited size to prevent too much insulin from being released in response to low and moderate stimulus intensities. Insulin secretion is well known to be regulated by reversible protein phosphorylation/dephosphorylation (Ämmälä et al., 1994Go). For example, agents that promote protein phosphorylation by activation of protein kinases A and C are strong stimuli of insulin secretion. Examples of physiological regulators that act via these protein kinases include the islet hormone glucagon (Renström et al., 1997Go), the incretin hormone GLP-1 (Lester et al., 1997Go), and acetylcholine (PKC) (Gao et al., 1994Go). Exactly how activation of PKA or PKC promotes secretion in the ß-cells remains unclear but their effects involve an increase in RRP. The results of Yang and Gillis (2004)Go and Wan et al. (2004)Go are significant also in this context, as they demonstrate that the size of HCSP increases several-fold (up to fourfold) in the presence of PKA/PKC activators as well as glucose, the major physiological stimulus of insulin secretion. Because of this effect, these compounds will appear to sensitize the secretory machinery to Ca2+, as first proposed 20 yr ago (Rorsman and Abrahamsson, 1985Go; Jones et al., 1986Go). The present studies make it likely that this effect results from an increase in HCSP size and not in the Ca2+ sensitivity of the release machinery itself. This represents a very significant advance of our knowledge about the control of insulin secretion. The data also explain the ability of the PKC activator PMA to stimulate insulin secretion even at subthreshold glucose concentrations when there is no electrical activity and [Ca2+]i is low (Bozem et al., 1987Go). The present data suggest this is because HCSP is released at a low rate already at resting [Ca2+]i in the presence of the PKC activator.

What is the way forward? It is becoming increasingly evident that high-affinity Ca2+-dependent exocytosis is a ubiquitous feature encountered in numerous (if not all) cells capable of regulated Ca2+-dependent exocytosis. Although it will certainly remain worthwhile to expand the catalog of cells in which HCSP contributes to the plasticity of secretion, it now becomes important to focus on the molecular mechanisms. One essential question that needs to be resolved is the differences in the Ca2+-sensing mechanisms involved in exocytosis of RRP (with a Kd of ~20 µM) and HCSP (Kd ~ 1 µM). As already noted above, both HCSP and RRP exocytosis involves SNARE proteins; but the widely different Ca2+ sensitivities of the two pathways suggest that they may not involve the same isoform of the calcium sensor synaptotagmin (Syt). It is in this context pertinent that rat insulin-secreting Ins1- and ß-cells contain the isoforms Syt III (Mizuta et al., 1994Go), V, and Syt IX (Iezzi et al., 2004Go). Future work should address the roles of these synaptotagmins in the two forms of Ca2+-induced exocytosis. One intriguing possibility is that phosphorylation of SNARE proteins somehow produces a switch from a low- to a high-affinity synaptotagmin isoform.


ACKNOWLEDGMENTS
Work in our own laboratories was supported by the Swedish Research Council, The European Commission, European Molecular Biology Organization and European Foundation for the Study of Diabetes. P. Rorsman is a Royal Society Wolfson Merit Award Fellow.


REFERENCES


    Ämmälä, C., L. Eliasson, K. Bokvist, P.O. Berggren, R.E. Honkanen, A. Sjöholm, and P. Rorsman. 1994. Activation of protein kinases and inhibition of protein phosphatases play a central role in the regulation of exocytosis in mouse pancreatic ß cells. Proc. Natl. Acad. Sci. USA. 91:4343–4347.[Abstract]

    Ämmälä, C., L. Eliasson, K. Bokvist, O. Larsson, F.M. Ashcroft, and P. Rorsman. 1993. Exocytosis elicited by action potentials and voltage-clamp calcium currents in individual mouse pancreatic B-cells. J. Physiol. 472:665–688.[Abstract]

    Aspinwall, C.A., L. Huang, J.R. Lakey, and R.T. Kennedy. 1999. Comparison of amperometric methods for detection of exocytosis from single pancreatic ß-cells of different species. Anal. Chem. 71:5551–5556.[CrossRef][Medline]

    Barg, S., X. Ma, L. Eliasson, J. Galvanovskis, S.O. Göpel, S. Obermüller, J. Platzer, E. Renström, M. Trus, D. Atlas, et al. 2001. Fast exocytosis with few Ca2+ channels in insulin-secreting mouse pancreatic B cells. Biophys. J. 81:3308–3323.[Abstract/Free Full Text]

    Bozem, M., M. Nenquin, and J.C. Henquin. 1987. The ionic, electrical, and secretory effects of protein kinase C activation in mouse pancreatic B-cells: studies with a phorbol ester. Endocrinology. 121:1025–1033.[Abstract]

    Braun, M., A. Wendt, B. Birnir, J. Broman, L. Eliasson, J. Galvanovskis, J. Gromada, H. Mulder, and P. Rorsman. 2004. Regulated exocytosis of GABA-containing synaptic-like microvesicles in pancreatic ß-cells. J. Gen. Physiol. 123:191–204.[Abstract/Free Full Text]

    Curry, D.L., L.L. Bennett, and G.M. Grodsky. 1968. Dynamics of insulin secretion by the perfused rat pancreas. Endocrinology. 83:572–584.[Medline]

    Gao, Z.Y., P. Gilon, and J.C. Henquin. 1994. The role of protein kinase-C in signal transduction through vasopressin and acetylcholine receptors in pancreatic B-cells from normal mouse. Endocrinology. 135:191–199.[Abstract]

    Henquin, J.C., and H.P. Meissner. 1984. Significance of ionic fluxes and changes in membrane potential for stimulus-secretion coupling in pancreatic B-cells. Experientia. 40:1043–1052.[Medline]

    Iezzi, M., G. Kouri, M. Fukuda, and C.B. Wollheim. 2004. Synaptotagmin V and IX isoforms control Ca2+-dependent insulin exocytosis. J. Cell Sci. 117:3119–3127.[Abstract/Free Full Text]

    Jones, P.M., J.M. Fyles, and S.L. Howell. 1986. Regulation of insulin secretion by cAMP in rat islets of Langerhans permeabilised by high-voltage discharge. FEBS Lett. 205:205–209.[CrossRef][Medline]

    Kasai, H. 1999. Comparative biology of Ca2+-dependent exocytosis: implications of kinetic diversity for secretory function. Trends Neurosci. 22:88–93.[CrossRef][Medline]

    Lester, L.B., L.K. Langeberg, and J.D. Scott. 1997. Anchoring of protein kinase A facilitates hormone-mediated insulin secretion. Proc. Natl. Acad. Sci. USA. 94:14942–14947.[Abstract/Free Full Text]

    Mizuta, M., N. Inagaki, Y. Nemoto, S. Matsukura, M. Takahashi, and S. Seino. 1994. Synaptotagmin III is a novel isoform of rat synaptotagmin expressed in endocrine and neuronal cells. J. Biol. Chem. 269:11675–11678.[Abstract/Free Full Text]

    Okazaki, K., I. Niki, S. Iino, S. Kobayashi, and H. Hidaka. 1994. A role of calcyclin, a Ca2+-binding protein, on the Ca2+-dependent insulin release from the pancreatic ß cell. J. Biol. Chem. 269:6149–6152.[Abstract/Free Full Text]

    Proks, P., L. Eliasson, C. Ämmälä, P. Rorsman, and F.M. Ashcroft. 1996. Ca2+- and GTP-dependent exocytosis in mouse pancreatic ß-cells involves both common and distinct steps. J. Physiol. 496:255–264.[Abstract]

    Renström, E., L. Eliasson, and P. Rorsman. 1997. Protein kinase A-dependent and -independent stimulation of exocytosis by cAMP in mouse pancreatic B-cells. J. Physiol. 502:105–118.[Abstract]

    Rorsman, P., and H. Abrahamsson. 1985. Cyclic AMP potentiates glucose-induced insulin release from mouse pancreatic islets without increasing cytosolic free Ca2+. Acta Physiol. Scand. 125:639–647.[Medline]

    Rorsman, P., and E. Renström. 2003. Insulin granule dynamics in pancreatic ß cells. Diabetologia. 46:1029–1045.[CrossRef][Medline]

    Takahashi, N., T. Kadowaki, Y. Yazaki, Y. Miyashita, and H. Kasai. 1997. Multiple exocytotic pathways in pancreatic ß cells. J. Cell Biol. 138:55–64.[Abstract/Free Full Text]

    Thoreson, W.B., K. Rabl, E. Townes-Anderson, and R. Heidelberger. 2004. A highly Ca2+-sensitive pool of vesicles contributes to linearity at the rod photoreceptor ribbon synapse. Neuron. 42:595–605.[CrossRef][Medline]

    Wan, Q.-F., Y. Dong, H. Yang, X. Lou, J. Ding, and T. Xu. 2004. Protein kinase activation increases insulin secretion by sensitizing the secretory machinery to Ca2+. J. Gen. Physiol. 124:653–662.[Abstract/Free Full Text]

    Wiser, O., M. Trus, A. Hernandez, E. Renström, S. Barg, P. Rorsman, and D. Atlas. 1999. The voltage sensitive Lc-type Ca2+ channel is functionally coupled to the exocytotic machinery. Proc. Natl. Acad. Sci. USA. 96:248–253.[Abstract/Free Full Text]

    Wollheim, C.B., S. Ullrich, P. Meda, and L. Vallar. 1987. Regulation of exocytosis in electrically permeabilized insulin-secreting cells. Evidence for Ca2+ dependent and independent secretion. Biosci. Rep. 7:443–454.[Medline]

    Yang, Y., and K.D. Gillis. 2004. A highly Ca2+-sensitive pool of granules is regulated by glucose and protein kinases in insulin–secreting INS-1 cells. J. Gen. Physiol. 124:641–651.[Abstract/Free Full Text]

    Yang, Y., S. Udayasankar, J. Dunning, P. Chen, and K.D. Gillis. 2002. A highly Ca2+-sensitive pool of vesicles is regulated by protein kinase C in adrenal chromaffin cells. Proc. Natl. Acad. Sci. USA. 99:17060–17065.[Abstract/Free Full Text]

    Yaseen, M.A., K.C. Pedley, and S.L. Howell. 1982. Regulation of insulin secretion from islets of Langerhans rendered permeable by electric discharge. Biochem. J. 206:81–87.[Medline]

    Zhu, H., B. Hille, and T. Xu. 2002. Sensitization of regulated exocytosis by protein kinase C. Proc. Natl. Acad. Sci. USA. 99:17055–17059.[Abstract/Free Full Text]




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