© Rockefeller University Press, 0022-1007/2003/8/433 $5.00
The Journal of Experimental Medicine, Volume 198, Number 3, 433-442

{gamma}{delta} T Cells Provide an Early Source of Interferon {gamma} in Tumor Immunity

Yunfei Gao1, Wancai Yang4, Meng Pan1, Eileen Scully1, Michael Girardi2, Leonard H. Augenlicht4, Joe Craft1,3 and Zhinan Yin1

1 Section of Rheumatology, Department of Medicine
2 Department of Dermatology, Yale School of Medicine, New Haven, CT 06520
3 Section of Immunobiology, Yale School of Medicine, New Haven, CT 06520
4 Department of Oncology, Montefiore Medical Center, Albert Einstein Cancer Center, Bronx, NY 10467

Address correspondence to Zhinan Yin, Section of Rheumatology, Yale School of Medicine, 300 Cedar Street, CAB Building Room S517, New Haven, CT 06520. Phone: 203-737-2772; Fax: 203-785-7053; email: zhinan.yin{at}yale.edu


   Abstract
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 
Interferon (IFN)-{gamma} is necessary for tumor immunity, however, its initial cellular source is unknown. Because {gamma}{delta} T cells primarily produce this cytokine upon activation, we hypothesized that they would provide an important early source of IFN-{gamma} in tumor immunosurveillance. To address this hypothesis, we first demonstrated that {gamma}{delta} T cell–deficient mice had a significantly higher incidence of tumor development after challenge with a chemical carcinogen methylcholanthrene (MCA) or inoculation with the melanoma cell line B16. In wild-type mice, {gamma}{delta} T cells were recruited to the site of tumor as early as day 3 after inoculation, followed by {alpha}ß T cells at day 5. We then used bone marrow chimeras and fetal liver reconstitutions to create mice with an intact {gamma}{delta} T cell repertoire but one that was specifically deficient in the capacity to produce IFN-{gamma}. Such mice had a higher incidence of tumor development, induced either with MCA or by inoculation of B16 melanoma cells, compared with mice with IFN-{gamma}–competent {gamma}{delta} T cells. Moreover, genetic deficiency of {gamma}{delta} T cells resulted in impaired IFN-{gamma} production by tumor antigen-triggered {alpha}ß T cell upon immunization with tumor lysate. These results demonstrate that {gamma}{delta} T cells can play a necessary role in tumor immunity through provision of an early source of IFN-{gamma} that in turn may regulate the function of tumor-triggered {alpha}ß T cells.

Key Words: cytokines • immunosurveillance • immune regulation • tumor immunosurveillance


   Introduction
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 
IFN-{gamma} is a necessary cytokine in the innate and adaptive immune responses that protect against tumor development (1, 2). Endogenously produced IFN-{gamma} forms the basis of a tumor surveillance system against the development of primary chemically induced sarcomas (3, 4). For example, mice deficient in the IFN-{gamma} receptor or in Stat1, required for IFN-{gamma} signaling, develop tumors more rapidly and with greater frequency in response to the chemical carcinogen methylcholanthrene (MCA)*when compared with wild-type mice. Lymphocytes also are necessary for immunity to spontaneously induced tumors, with their role dependent upon IFN-{gamma} synthesis (5). Tumor cells themselves are the major target of the action of IFN-{gamma}, as determined using IFN-{gamma}–insensitive tumor cell lines and a transplant tumor model (6).

Several immunological and nonimmunological mechanisms have been proposed to explain the effect of IFN-{gamma} in tumor immunity (1). Perhaps most importantly, IFN-{gamma} is critical for the development of an effective adaptive immune response, primarily directing the balance of Th1/Th2 cytokines toward protection. Notably, the primary cellular source for the initial production of IFN-{gamma} has not yet been defined. It has been proposed that innate cells or innate-like lymphocytes, such as NK, NK T, and {gamma}{delta} T cells, might provide the early source of this cytokine (2).

{gamma}{delta} T cells have many features that distinguish them from {alpha}ß T cells (79). Most of the former have a phenotype of spontaneous activation with a fast turnover rate in vivo (10), and they quickly expand upon pathogen challenge in the first few days after infection (1114). {gamma}{delta} T cells also regulate CD4+ and CD8+ T cell function. For example, in a Coxsackie virus infection model, V{gamma}1 cells direct CD4+ T cells toward a dominant Th2 response, whereas V{gamma}4 cells bias them toward a dominant Th1 response (15). In a similar manner, {gamma}{delta} T cells are responsible for the establishment of a protective CD8+ memory response during Listeria monocytogenes infection (16), whereas {gamma}{delta} T cell–deficient mice have an inadequate CD8+ T cell immune response against Encephalitozoon cuniculi infection (17).

{gamma}{delta} T cells provide the host with several potential advantages over conventional lymphocytes in host protection against tumors. CD8+ T cells recognize processed peptide antigens in the context of self-MHC class I molecules that may have impaired expression on tumor cells, thereby rendering the tumor immunologically invisible to {alpha}ß T cells. By contrast, {gamma}{delta} T cells recognize antigens directly, with no requirement for antigen processing or presentation (8, 9). More recently, {gamma}{delta} T cells have been shown to recognize the MHC class I–related molecules MICA and MICB, which are induced by stress resulting from infection or injury, or by cellular transformation (18, 19). These molecules have been shown to be stimulatory ligands for the NK cell receptor NKG2D that is also found on {gamma}{delta} T cells (20, 21). Moreover, skin-associated {gamma}{delta} T cells play an important role in preventing tumor development through NKG2D-mediated cytolytic mechanisms (22).

To precisely define the role of {gamma}{delta} T cells in the protective immune responses against tumor development, we analyzed tumor formation and growth in B6 TCR{delta}-deficient (TCR{delta}-/-) mice compared with wild-type mice, as well as in bone marrow chimeras or fetal liver–reconstituted mice in which {gamma}{delta} T cells selectively lack the ability to produce IFN-{gamma}. We demonstrate that {gamma}{delta} T cells play a critical role in the protective immune response against tumor development by providing an important early source of IFN-{gamma} that in turn regulates {alpha}ß T cell effector function.


   Materials and Methods
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 
Mice.
C57BL/6J (B6), C57BL/6J-Tcrbtm1Mom (B6 TCRß-deficient mice [TCRß-/-]), C57BL/6J-Tcrdtm1Mom (B6 TCR{delta}-/-), and C57BL/6-Ifngtm1Ts (B6 IFN-{gamma}–deficient [IFN-{gamma}-/-]) mice were purchased from The Jackson Laboratory. B6 IFN-{gamma}-/- mice and B6 TCRß-/- mice were bred to create B6 TCRß-/- IFN-{gamma}-/- double deficient mice. For bone marrow chimeras, the donors and recipients are listed in Table I. All animals were maintained under specific pathogen-free conditions and used at 6–8 wk of age unless specifically mentioned.

Reagents.
Recombinant murine IL-2 and IL-12 were purchased from R&D Systems. Anti–mouse mAb used for phenotypic and cytokine analyses were all purchased from BD Biosciences.

Tumor Models.
Induction of fibrosarcomas by injection with MCA has been described (3, 22). In brief, MCA was dissolved in peanut oil overnight and sex- and age-matched groups of mice were injected intradermally in the flank (75 µg MCA/mouse). Fibrosarcoma development was recorded weekly. Alternatively, B16 F0 melanoma cells (provided by M. Mamula, Yale School of Medicine, New Haven, CT) were injected subcutaneously (5 x 104 tumor cells/ mouse) and tumor growth was observed and recorded daily for over 3 wk.

Bone Marrow Chimeras.
Bone marrow chimeras were prepared according to a previously described protocol (23). In brief, recipients were twice irradiated with 500 rads, with a 3-h interval between doses (total 1,000 rads). Donor bone marrow cells (mixed equally from two donors, see Table I for details) were injected intravenously into recipients (107 cells/ mouse). Antibiotics were added to the drinking water for the first 5 wk after reconstitution. At 12 wk after reconstitution, mice were injected intradermally in the flank with MCA or subcutaneously with B16 melanoma cells and monitored for tumor development as described above. At the time of death, splenocytes were used to examine T cell composition and determine the ability of T cell subsets ({alpha}ß vs. {gamma}{delta} T cells) to produce IFN-{gamma} as described below.

Fetal Liver Reconstitution.
Fetal liver cell reconstitution was performed as previously described (24). Accordingly, donor cells were isolated from the fetal livers at 14 d of gestation, and 107 unfractioned fetal liver cells in sterile PBS were injected intraperitoneally into 3-d-old recipients. After 8 wk of reconstitution, mice were used for tumor implantation as described above. At the time of sacrifice (3 wk after tumor inoculation), splenocytes were used to examine T cell composition and determine the ability of T cell subsets ({alpha}ß and {gamma}{delta} T cells) to produce IFN-{gamma} as described below.

Cell Composition and Intracellular Cytokine Staining.
To test the composition of T cell subsets from bone marrow chimeras and from mice reconstituted with fetal liver cells, splenocytes were stained with anti-CD3 (CyChrome), anti–{gamma}{delta} TCR (FITC), and anti–{alpha}ß TCR (PE) followed with FACS® analysis. To confirm the success of the selective depletion of the ability of {gamma}{delta} T cells to produce IFN-{gamma}, 2 x 106/ml splenocytes were cultured with 10 µg/ml anti-CD3 and 1 µg/ml anti-CD28 in the presence of 5 ng/ml IL-12 as described in our previous studies (25). After 4 d of culture, cells were restimulated with anti-CD3 and anti-CD28 in the presence of brefeldin A for 6 h for intracellular IFN-{gamma} staining as detailed below.

Intracellular Cytokine Staining.
For bone marrow chimeras and for mice reconstituted with fetal liver cells, splenocytes cultured as described above were activated with anti-CD3 and anti-CD28 for 6 h with brefeldin A added for the last 2 h. Cells were stained with CyChrome anti-{alpha}ß, and FITC anti–{gamma}{delta} TCR antibodies followed with fixation in 2% formaldehyde in PBS. Cells were then permeabilized and stained with PE anti–IFN-{gamma} as previously described (25). Gating was performed on {alpha}ß and {gamma}{delta} T cells and the percentage of IFN-{gamma}+ cells was reported.

Immunohistochemical Staining.
Melanoma cells (B16-F0) were inoculated into the right flank of C57BL/6 mice at the age of 7 wk. The mice were killed at various time points after injection (days 1, 3, 5, 7, 9, and 11, respectively). The injected sites, including skin and subcutaneous tissues and tumors per se, were resected and cut across the center. Tissues were then embedded in OCT compound, frozen in liquid nitrogen, and mounted for cryostat sectioning for immunohistochemical staining. 8-µm cryostat sections were fixed in cold acetone for 10 min and rinsed three times with cold PBS for 10 min to remove OCT. Endogenous peroxidase was quenched with 1.5% H2O2 for 10 min at room temperature. The sections were blocked with 10% normal goat serum for 30 min in a humidified chamber and then incubated with primary antibodies against TCR-{gamma}{delta}, CD4, and CD8{alpha} for 2 h at room temperature. After washing twice, a biotinylated secondary antibody was added and incubated for 30 min, followed by incubation with ABC for 30 min and the substrate 3',3' diaminobenzidine for 5 min, combined with hematoxylin counterstaining for 10 s. Sections of B6 mouse spleen were incubated with anti–mouse TCR-{gamma}{delta}, CD4, and CD8{alpha} antibodies as positive controls. Normal goat serum without primary antibodies was used as a negative control. In addition, spleen tissues of TCR{delta}-/- mouse were stained with anti–mouse TCR-{gamma}{delta} as an additional negative control. Positive cells were counted in five random high power fields under a Nikon microscope.

Preparation of Cell Suspensions from the Site of Tumor Injection.
C57BL/6 mice (n = 10) at age 7 wk were injected with 105 B16F0 melanoma cells. At day 3 after inoculation, small pieces of shaved skin were resected and digested with trypsin-GNK solution (0.29% trypsin, 0.86% NaCl, 0.041% KCl, and 0.1% glucose) at 37°C for 2 h, and then further treated with collagenase/hyaluronidase digestion solution buffer (0.27% collagenase, 0.025% hyaluronidase, 1% DNase, 0.01% Hepes, 0.01% sodium pyruvate in RPMI) for 2 h at 37°C as previously described (26). The digested skin was filtered through a 40-mM cell strainer (Becton Dickinson) and a single cell suspension was obtained containing resident cells and infiltrating cells (26). Cells were stained with anti–CD3-CyChrome and anti–{gamma}{delta} TCR FITC, and cells were then fixed with 2% formaldehyde and further stained for intracellular IFN-{gamma} (anti–IFN-{gamma} PE) as described above.

Tumor Antigen–triggered {alpha}ß T Cell IFN-{gamma} Production.
Cultured B16 F0 cells were digested with trypsin and washed with PBS, and then sonicated and centrifuged. The protein concentration of the supernatant was determined by a Bio-Rad kit (Bio-Rad Laboratories) and adjusted to 2 mg/ml. Age- and sex-matched B6 and B6 TCR{delta}-/- mice were immunized with 100 µg tumor lysate emulsified in CFA in the hind footpad and rechallenged with the same concentration of tumor lysate emulsified in IFA 14 d later. On day 21, the mice were killed and draining lymph node cells were cultured with 100 µg/ml tumor lysate. Intracellular cytokine staining for the analysis of IFN-{gamma} production by CD4+ and CD8+ T cells was performed as described above.

Statistics.
Statistical significance was evaluated by two-tailed, unpaired Student's t test or nonparametric analysis if SD were significantly different between the two compared groups using software InStat 2.03 for Macintosh (GraphPad Software). The incidence of tumor development was compared and analyzed using the log rank test, performed by GraphPad Prism Version 3.0a for Macintosh (GraphPad Software). Throughout the text, figures, and legends, the following terminology was used to denote statistical significance: *, P < 0.0001 or P < 0.001; **, P < 0.01; ***, P < 0.05.


   Results
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 
{gamma}{delta} T Cells Are Critical for Preventing Primary and Transplantable Tumor Development.
Both lymphocytes and IFN-{gamma} are critical for tumor immunosurveillance, with overlapping effects (5). {gamma}{delta} T cells are critical for preventing MCA-induced fibrosarcoma development in susceptible strains of mice (22). To test the role of {gamma}{delta} T cells in preventing tumor development in a resistant strain, sex- and age-matched B6 wild-type (n = 28) and B6 TCR{delta}-/- mice (n = 24) were injected with MCA in the flank (75 µg/mouse), and tumor development was observed and measured weekly. Mice lacking {gamma}{delta} T cells had a significantly greater susceptibility to MCA-induced tumors, with 80% of the mice showing tumor development (tumor size larger than 4 x 4 mm) when compared with wild-type TCR{delta}+/+ mice (20%; Fig. 1 A, p < 0.01). Tumors between 2 x 4 mm without progression in 3 wk were excluded from analysis, as these may result from neovascularization, a IFN-{gamma}–mediated process (2).



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Figure 1. {gamma}{delta} T cells are necessary to prevent tumor development. (A) Protective role of {gamma}{delta} T cells in the MCA-induced tumor model. Sex- and age-matched B6 wild-type (WT, n = 28) and B6 TCR{delta}-deficient mice (TCR{delta}-/-, n = 24) were injected with 75 µg MCA/mouse in the flank. Tumor development was recorded weekly after injection. Tumor size >4 x 4 mm was considered positive as previously described (reference 3). (B) Protective role of {gamma}{delta} T cells and IFN-{gamma} in the B16 melanoma transfer model. Four groups of age- and sex-matched mice (B6 wild-type mice, n = 12; B6 TCRß-deficient mice, n = 18; B6 TCR{delta}-deficient mice, n = 21; B6 IFN-{gamma}–deficient mice, n = 15), all at 8 wk of age were injected subcutaneously with 5 x 104 B16 F0 melanoma cells and tumor growth was recorded daily. The percentage of mice with tumors 21 d after injection is shown. Data represent mean ± SD of three independent experiments. *, P < 0.001; **, P < 0.01.

 
To further explore the protective role of {gamma}{delta} T cells in a transplantable tumor model, T cell–intact (WT, n = 12) or T cell subset–deficient mice (TCRß-/-, n = 18; TCR{delta}-/-, n = 21) and IFN-{gamma}-/- mice (n = 15) were injected with the melanoma cell line B16 F0 subcutaneously at low doses (5 x 104 cells/mouse). In preliminary studies, titration of the number of injected tumor cells in B6 mice demonstrated that with this dose of tumor cells, only 20–30% of T cell–intact, wild-type mice showed tumor growth (unpublished data). Both {alpha}ß and {gamma}{delta} T cells contributed to the protective immune response against tumor development in vivo, indicating a nonredundant role for {gamma}{delta} T cells in transplantable melanoma growth (Fig. 1 B). Consistently, IFN-{gamma}-/- mice also showed a higher susceptibility to tumor growth, reaffirming the critical role of IFN-{gamma} in immunosurveillance (3, 5, 6).

{gamma}{delta} T Cells Are Recruited to the Tumor Injection Site Earlier Than {alpha}ß T Cells and Produce IFN-{gamma} Ex Vivo.
{gamma}{delta} T cells respond to pathogen invasion and quickly expand in the first few days of infection, and thus these T cells may contribute to early pathogen protection (1114). To investigate whether {gamma}{delta} T cells are early infiltrating immune cells responding to tumor invasion, a series of tissue samples of B16 melanoma injection sites were analyzed by immunohistochemical staining using antibodies against {gamma}{delta} TCR or CD4+ or CD8+ T cells. TCR-{gamma}{delta}+ cells were observed in subcutaneous tissue as early as day 3, and the infiltration of these cells gradually increased and peaked at day 7 (Fig. 2 A, top). In contrast, CD4+ or CD8+ T cell infiltration was not observed at day 3, although these {alpha}ß T cells appeared at day 5 and gradually increased in number until reaching a peak at day 11 (Fig. 2 A, middle and bottom, and B). To test whether the infiltrating {gamma}{delta} T cells produce IFN-{gamma} in situ, B6 mice were injected with B16 melanoma cells, the tissue at the tumor injection site was digested, and infiltrating cells were stained directly for anti–{gamma}{delta} TCR and anti-CD3 followed by intracellular IFN-{gamma} staining. Consistent with our immunohistological staining, most of the infiltrating CD3+ lymphocytes were {gamma}{delta} T cells (Fig. 2 C). A minor population of CD3+ T cells (0.19%) might be NK T cells because they did not stain positive for {alpha}ß TCR (unpublished data). Strikingly, most IFN-{gamma}–producing cells were {gamma}{delta} T cells and only 0.07% of IFN-{gamma}1 cells were from another cell source (Fig. 2 D). These results imply that {gamma}{delta} T cells infiltrate tumor sites earlier than {alpha}ß T cells, providing IFN-{gamma} production that may guide the function of {alpha}ß T cells.





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Figure 2. {gamma}{delta} T cells are recruited earlier than {alpha}ß T cells into tumor sites. B6 wild-type mice were inoculated with B16 F0 melanoma cells (5 x 104 cells/mouse) and at different time points after inoculation, the tumor injection site was frozen and stained with specific antibodies against TCR {gamma}{delta}, CD4, and CD8 as described in Materials and Methods. (A) One slide from each time point depicting the staining for each cell type is shown. Examples of positive cells are indicated by arrows. (B) Summary of mean ± SD of the number of positive cells per high power field is shown. (C) Cell suspensions were prepared from pools of 10 tissues collected from tumor injection sites and cells were stained with anti-CD3 and anti–{gamma}{delta} TCR. A representative example of a flow cytometry plot is shown. (D) Stained cells (from C) were fixed and permeabilized for intracellular IFN-{gamma} (PE) staining. A representative plot is shown.

 
{gamma}{delta} T Cells Provide an Early Source of IFN-{gamma} for a Protective Immune Response Against Both Primary Tumor Induction and a Transferred Tumor.
Based upon our previous findings that {gamma}{delta} T cells predominantly produce IFN-{gamma} upon activation (25, 27), and on the fact that IFN-{gamma} plays an essential role in tumor immunosurveillance (13), we hypothesized that the {gamma}{delta} T cells that were visualized at the tumor site might play an important protective role through IFN-{gamma} production. To test this hypothesis, we first prepared bone marrow chimeras in which T cell composition is intact (both {alpha}ß and {gamma}{delta} T cells), except that the {gamma}{delta} T cells could ({gamma}{delta} IFN-{gamma}+) or could not ({gamma}{delta} IFN-{gamma}-) produce IFN-{gamma}. The detailed composition of donors and recipients is shown in Table I, with {gamma}{delta} IFN-{gamma}- animals labeled as the experimental group and the {gamma}{delta} IFN-{gamma}+ mice as the control group. As expected, experimental chimeras ({gamma}{delta} IFN-{gamma}2) were fully reconstituted with {alpha}ß and {gamma}{delta} T cells, with the same ratios of {alpha}ß and {gamma}{delta} T cells as wild-type controls (Fig. 3 A). Moreover, upon activation and restimulation in vitro, only {alpha}ß (52.7%), but not {gamma}{delta} (0%), T cells produced IFN-{gamma} (Fig. 3 B), indicating the success of obtaining the expected phenotype in this group. The cell composition was very similar in the control group of chimeras ({gamma}{delta} IFN-{gamma}1), although by contrast to the experimental group, their {gamma}{delta} T cells were fully capable of IFN-{gamma} production as expected (unpublished data).


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Table I. Generation of Bone Marrow Chimeras

 


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Figure 3. Generation and analysis of bone marrow chimeras. (A) Composition of {alpha}ß and {gamma}{delta} T cells in wild-type mice versus an experimental group of bone marrow chimeras. Bone marrow chimeras were prepared as described in Materials and Methods using donors and recipients (refer to Table I). At the end of the experiments, splenocytes from the experimental group and wild-type mice were prepared and stained with FITC anti–{gamma}{delta} TCR (GL3), PE anti–{alpha}ß TCR (H57-597), and CyChrome anti-CD3 (145-2C11). After gating on CD3+ T cells, the composition of {gamma}{delta} and {alpha}ß T cells was analyzed. Two controls and six reconstituted mice are shown. (B) IFN-{gamma} production by {gamma}{delta} and {alpha}ß T cells in bone marrow chimeras. Splenocytes from bone marrow chimeras (experimental group, {gamma}{delta} IFN-{gamma}2) were cultured with anti-CD3 and anti-CD28 in the presence of IL-12 for 5 d. Cells were then restimulated with anti-CD3 and anti-CD28 in the presence of brefeldin A for 6 h. After staining with FITC anti–TCR {gamma}{delta} and CyChrome anti–TCR {alpha}ß, cells were fixed, permeabilized, and further stained with PE anti–IFN-{gamma}. After gating on {alpha}ß and {gamma}{delta} T cells, the percentage of IFN-{gamma}+ cells was detected (see representative histogram).

 
Next, we used these chimeras to explore the role in tumor protection of IFN-{gamma} produced solely by {gamma}{delta} T cells. Here we compared tumor development in reconstituted animals (n = 8–9, 12 wk after reconstitution) to sex- and age-matched B6 wild-type (n = 10), B6 TCRß-/- (n = 13), B6 TCR{delta}-/- (n = 11), and B6 IFN-{gamma}-/- (n = 19) mice. At 14 wk after injection with MCA, 70% TCRß-/- and TCR{delta}-/- mice showed tumor development (P < 0.05), compared with 30% of wild-type animals (Fig. 4 A, P < 0.05), confirming a nonredundant role for {gamma}{delta} T cells in preventing tumors (22). In addition, we observed a significantly higher percentage of tumors in the IFN-{gamma}-/- mice (Fig. 4 A, P < 0.01), consistent with previous findings that IFN-{gamma} is essential in tumor Immunosurveillance in the MCA-induced tumor model (3).




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Figure 4. IFN-{gamma} produced by {gamma}{delta} T cells is critical for protective immune responses against tumors. (A) Development of MCA-induced tumors in bone marrow chimeras and in unmanipulated mice. Four groups of age- and sex-matched mice (B6 wild-type mice, n = 10; B6 TCRß-deficient mice, n = 13; B6 TCR{delta}-deficient mice, n = 11; B6 IFN-{gamma}–deficient mice, n = 19; all at age 6 wk), and two groups of age- and sex-matched bone marrow chimeras (experimental group, n = 9; control group, n = 8; 10 wk after reconstitution) were injected with MCA (75 µg/mouse in the flank). Animals were observed weekly and tumor size was measured with a calimeter. The percentage of mice that developed tumors is shown. Data represent mean ± SD of three independent experiments. *, P < 0.001; **, P < 0.01. (B) B16 melanoma model in bone marrow chimeras. Bone marrow chimeras reconstituted as in A were injected with 5 x 104 B16 F0 cells/mouse and tumor growth was recorded. The percentage of mice that developed tumors 21 d after inoculation is shown. Data represent mean ± SD of three independent experiments. *, P < 0.001; **, P < 0.01. (C) B16 melanoma model in mice reconstituted with fetal liver cells. TCR{delta}-/- mice were reconstituted with fetal liver cells either from B6 TCRß-/- mice ({gamma}{delta}+ IFN-{gamma}+) or from TCRß-/- IFN-{gamma}-/- ({gamma}{delta}+ IFN-{gamma}-). 8 wk after reconstitution, mice were inoculated with 5 x 104 B16 F0 cells/mouse and tumor growth was recorded. The percentage of mice that developed tumors 21 d after inoculation is shown. Data represent mean ± SD of three independent experiments. *, P < 0.001; **, P < 0.01. (D) Time course of B16 tumor development in bone marrow chimeras (B) and fetal liver reconstituted mice (C).

 
Strikingly, the experimental group of chimeras, in which the only defect was the ability of {gamma}{delta} T cells to produce IFN-{gamma} ({gamma}{delta} IFN-{gamma}-), had an incidence of tumor development similar to IFN-{gamma}-/- mice (completely lacking IFN-{gamma}), indicating the importance of IFN-{gamma} produced by {gamma}{delta} T cells in preventing tumor development. Moreover, the experimental group of chimeras had a significantly higher tumor incidence than control chimeras ({gamma}{delta} IFN-{gamma}+) or wild-type mice (Fig. 4 A, P < 0.01), although mice from the experimental group only differed from control chimeras or wild-type mice in the ability of their {gamma}{delta} T cells to produce IFN-{gamma}. Thus, our results provide strong evidence for a necessary role of {gamma}{delta} T cells in providing a source of IFN-{gamma} for the protective effect provided by this cytokine.

To further substantiate the requirement for {gamma}{delta} T cells in provision of an early source of IFN-{gamma} in tumor protection, we again turned to the B16 melanoma transplantable tumor model. Sex- and age-matched bone marrow chimeras (experimental: {gamma}{delta} IFN-{gamma}-, n = 9; control: {gamma}{delta} IFN-{gamma}+, n = 6) were inoculated with B16 F0 melanoma cells (5 x 104 cells/mouse) and tumor growth was monitored daily. At day 21 after inoculation, >50% (6 out of 9) of the experimental ({gamma}{delta} IFN-{gamma}-) group developed tumors (>4 x 4 mm), whereas only 17% of control chimeras ({gamma}{delta} IFN-{gamma}+, 1 out of 6) showed sizeable tumor growth (Fig. 4 B, P < 0.01). Moreover, {gamma}{delta} IFN-{gamma}2 bone marrow chimeras developed tumors much earlier when compared with {gamma}{delta} IFN-{gamma}1 chimeras (Fig. 4 D, P < 0.05). This further supports the notion that {gamma}{delta} T cells are a critical source of IFN-{gamma} in preventing tumor growth in vivo.

Finally, to more firmly establish the role of {gamma}{delta} T cells in providing the early source of IFN-{gamma} in tumor immunosurveillance, we also reconstituted {gamma}{delta} T cell–deficient mice with fetal liver cells from mice with an intact {gamma}{delta} T cell repertoire, but with or without the capacity to produce IFN-{gamma}. The advantage of this system is that T cell subsets distribute and repopulate similarly to wild-type mice (24). Moreover, reconstitution takes place as mice mature (during the 5–6 wk after the birth), so that experiments can be performed at ages of 6–8 wk. TCR{delta}-/- mice at 3 d of birth were reconstituted with fetal liver cells (14 d of gestation) either from TCRß-/- mice ({gamma}{delta} T cells can produce IFN-{gamma}; hence, mice are {gamma}{delta} IFN-{gamma}+) or from TCRß-/- IFN-{gamma}-/- mice ({gamma}{delta} T cells cannot produce IFN-{gamma}; hence, mice are {gamma}{delta} IFN-{gamma}-). 8 wk after reconstitution, sex- and age-matched mice from both groups ({gamma}{delta} IFN-{gamma}+, n = 20; {gamma}{delta} IFN-{gamma}-, n = 18), along with unmanipulated B6 wild-type and B6 TCR{delta}-/- mice, were injected with B16 F0 melanoma cells (5 x 104 cells/mouse) and tumor growth was observed daily. {gamma}{delta} IFN-{gamma}- mice showed a significantly higher rate of tumor growth than {gamma}{delta} IFN-{gamma}+ reconstituted mice (Fig. 4 C, P < 0.01), with significantly earlier kinetics of tumor development (Fig. 4 D, P < 0.01), providing further support for the concept that {gamma}{delta} T cells provide a necessary source of IFN-{gamma} in protective immunity against tumors.

{gamma}{delta} T Cells Regulate Tumor Antigen–triggered {alpha}ß T Cell IFN-{gamma} Production.
IFN-{gamma} is a critical cytokine for directing both CD4+ and CD8+ T cell differentiation (28, 29). These T cells regulate CD4+ and CD8+ T cell effector function upon pathogen infection (16, 17). Based upon the results described above, we next hypothesized that {gamma}{delta} T cells might regulate tumor antigen–triggered {alpha}ß T cell IFN-{gamma} production. To address this question, sex- and age-matched B6 wild-type and B6 TCR{delta}-/- mice (n = 5 for both groups) were immunized with B16 F0 cell lysates, followed by challenge with identically prepared lysates in IFA 14 d later. The production of IFN-{gamma} by {alpha}ß T cells in draining lymph nodes was then analyzed 7 d later upon restimulation with tumor lysate in vitro. Both CD4+ and CD8+ T cells from TCR{delta}-/- mice produced significantly less IFN-{gamma} upon tumor antigen restimulation in vitro compared with wild-type mice (Fig. 5 A). The finding is consistent with the previous findings that {gamma}{delta} T cells are required to promote IFN-{gamma} production by both CD4+ and CD8+ T cells (15, 17).



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Figure 5. {gamma}{delta} T cells regulate IFN-{gamma} production by tumor antigen–triggered {alpha}ß T cells. B6 and B6 TCR{delta}-/- mice (n = 5 for both strains) were immunized with tumor lysates in CFA and rechallenged in vitro with identically prepared lysates for 24 h. Brefeldin A was added for the last 4 h of culture and cells were then used for intracellular cytokine staining. After gating on CD3+ and CD8+ T cells, the percentage of IFN-{gamma}–producing cells is shown. (A) The percentage of IFN-{gamma}–producing cells from both CD8+ and CD4+ cells is shown (CD8-, lower left quadrant of dot plot shown in B). Data is mean ± SD of five different mice. **, P < 0.01; ***, P < 0.05. (B) One example of intra-cellular cytokine staining from wild-type (WT) and TCR{delta}-/- mice is shown.

 

   Discussion
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 
It is well established that IFN-{gamma} is an essential mediator for the protective immune response against tumor development (1, 3, 5). However, the source of this cytokine early after tumor challenge has not been identified. NK cells and certain lymphocytes that have features of both innate and adaptive immune cells, including NK T and {gamma}{delta} T cells, are considered to be important for bridging the innate and adaptive immune responses and they might provide the initial source of IFN-{gamma} in tumor protection. This in turn could facilitate the cascade of the adaptive immune response and augment later IFN-{gamma} production (2). In this report, we demonstrate that {gamma}{delta} T cells play a critical role in protective immune responses against tumor development by providing such an early source of IFN-{gamma}, which in turn regulates tumor antigen–triggered CD4+ and CD8+ T cell responses.

We first demonstrated that {gamma}{delta} T cells play a nonredundant role in preventing tumor formation and tumor growth in both primary and transplantable tumor models. {gamma}{delta} T cells have been convincingly shown in many systems to play a critical role in early pathogen protection (11, 30, 31). Recently, Girardi et al. (22) have demonstrated a necessary role for {gamma}{delta} T cells in cutaneous malignancy using TCR{delta}-/- mice, with NKG-2D–mediated cytolytic activity proposed as the antitumor mechanism. In agreement with these results, here we show that {gamma}{delta} T cells are able to retard tumor growth in both a MCA fibrosarcoma (Fig. 1 A) and a melanoma model (Fig. 1 B). A lack of {gamma}{delta} T cells rendered mice significantly more susceptible to a chemical carcinogen and to tumor cell inoculation compared with wild-type mice. Moreover, TCR{delta}-/- mice had a tumor incidence similar to TCRß-/- and IFN-{gamma}-/- mice, indicating a nonredundant role for {gamma}{delta} T cells in protective tumor immune responses.

The most important finding presented in this report is that IFN-{gamma} produced by {gamma}{delta} T cells is critical for a protective immune response against tumor development. Several of our findings support this notion. First, {gamma}{delta} T cells were the first group of T cells recruited into tumor injection site (Fig. 2). It has been demonstrated in many infection systems that {gamma}{delta} T cells are among the first group of cells to expand and infiltrate at inflammatory sites (32, 33). Our report finds for the first time that {gamma}{delta} T cells respond to tumor cell insults earlier than {alpha}ß T cells. Moreover, these early infiltrating {gamma}{delta} T cells (at day 3 after inoculation) produce IFN-{gamma} ex vivo in the absence of extra stimulation (Fig. 2 D), strongly suggesting that the early source of IFN-{gamma} is from {gamma}{delta} T cells. However, staining of tumor sites for IFN-{gamma} was negative (unpublished data), likely a consequence of the small amounts of locally produced cytokine or due to the low avidity of anti–IFN-{gamma} antibodies. Nevertheless, our results, together in the setting of the ability of {gamma}{delta} T cells to recognize antigens without the need for engagement of conventional antigen processing pathways and their default production of IFN-{gamma}, led us to believe that the early interaction between {gamma}{delta} T cells and tumor cells might provide the early source of IFN-{gamma} for subsequent {alpha}ß T cell activation and differentiation.

To directly address this issue, we constructed mice with an intact {alpha}ß and {gamma}{delta} T cell composition, except that the latter lacked the ability to produce IFN-{gamma}. The advantage of these animals is that we could directly assess our hypothesis that the IFN-{gamma} produced by {gamma}{delta} T cells is a critical early source for tumor immunity, in a physiological setting. Our bone marrow chimeras had a composition of {alpha}ß and {gamma}{delta} T cells similar to wild-type mice (Fig. 3 A), although we produced animals in which {gamma}{delta} T cells could or could not produce IFN-{gamma} (Fig. 3 B). A similar phenotype was observed in mice selectively reconstituted with fetal liver cells (unpublished data). Using these mice, we demonstrated that IFN-{gamma} made by {gamma}{delta} T cells resulted in susceptibility to both MCA-induced tumors (Fig. 4 A) and B16 melanoma cell inoculation (Fig. 4, B–D). This finding strongly supports our hypothesis that {gamma}{delta} T cells are indeed the early source of IFN-{gamma} for protective immune responses against tumor formation and tumor growth.

Finally, IFN-{gamma} produced by {gamma}{delta} T cells appeared to modulate CD4+ and CD8+ IFN-{gamma} production. Depletion of {gamma}{delta} T cells resulted in significantly reduced IFN-{gamma} production by both CD4+ and CD8+ T cells upon challenge with tumor antigen (Fig. 5), indicating that early production of IFN-{gamma} by {gamma}{delta} T cells enhanced subsequent IFN-{gamma} production by {alpha}ß T cells. Without {gamma}{delta} T cell synthesis of IFN-{gamma}, the adaptive immune response was impaired, leading to enhanced tumor susceptibility.

NK and NK T cells have also been shown to play an important role in tumor immunosurveillance, in part dependent on IFN-{gamma} production (3436). Although NK and NK T cells are intact in our TCR{delta}-/- mice, the fact that these mice remain highly susceptible to MCA or B16 melanoma inoculation may be explained by the following possibilities. First, NK and NK T cells might be the down-stream targets of {gamma}{delta} T cells and {gamma}{delta} T cells might be required for the competent function of these cells, through direct interaction or provision of IFN-{gamma}. Second, {gamma}{delta} T cells might be the target effector cells of NK and NK T cells. Without {gamma}{delta} T cells the effector function of NK and NKT cells might be impaired. Further analysis of the functional states of NK or NK T cells in TCR{delta}-/- mice or in our mice with selective depletion of IFN-{gamma} produced by {gamma}{delta} T cells should further our understanding of the interplay between these cell populations.

It should be emphasized that {gamma}{delta} T cells might have other antitumor mechanisms other than provision of IFN-{gamma} for protective immune responses. {gamma}{delta} T cells from synovial fluid of patients with Lyme arthritis are cytolytic toward a wide array of target cells by a Fas–FasL interaction (37), and human intestinal V{delta}1 {gamma}{delta} T cells directly recognize tumor cells of epithelial origin (38). Recently, it has been demonstrated that skin {gamma}{delta} T cells directly kill skin carcinoma cells by an NKG-2D-Rae1/H60 interaction (22). However, it is unclear at the present time whether {gamma}{delta} T cell cytolytic function is correlated with, or requires, IFN-{gamma} production. Further studies are needed to clarify whether these two effector arms overlap or if they are in parallel.

In summary, we have presented evidence that IFN-{gamma} produced by {gamma}{delta} T cells is an essential early source of IFN-{gamma} for tumor immunosurveillance, which in turn regulates {alpha}ß T cell IFN-{gamma} production. These findings will not only shed light on the molecular mechanisms of immunosurveillance, but may also help in the design of novel therapeutic approaches via manipulation of the function of {gamma}{delta} T cells.


   Acknowledgments
 
We thank Ping Zhu and Christie Hawley for animal care. We thank Dr. Richard Bucala, Dr. Elisabeth Ramsburg, Dr. Daniel H. Kaplan, and Dr. Mark Mamula for critical review the manuscript. We greatly appreciate the kind help provided by Dr. R.E. Tigelaar and Dr. Julia Lewis for preparation of skin cells.

This work was supported in part by an Arthritis Foundation Investigator Award, a National Institutes of Health (NIH; NIAMS) K01 AR 02188 grant, and a Yale Skin Disease Research Center PF grant (to Z. Yin). It was also supported by grants AR40072 and AR44076 from the NIH, by the Arthritis Foundation, the Lupus Foundation of America and their Connecticut chapters, the S.L.E. Foundation, Inc., and a Kirkland Scholars Award (to J. Craft).

Submitted: April 10, 2003
Revised: May 20, 2003
Accepted: June 10, 2003


   Footnotes
 
* Abbreviation used in this paper: MCA, methylcholanthrene. Back


   References
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 

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