By
From * the Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Parkville
3050, Australia; and Roche Milano Ricerche, 1-20132, Milano, Italy
The class II major histocompatibility complex molecule I-Ag7 is strongly linked to the development of spontaneous insulin-dependent diabetes mellitus (IDDM) in non obese diabetic mice
and to the induction of experimental allergic encephalomyelitis in Biozzi AB/H mice. Structurally,
it resembles the HLA-DQ molecules associated with human IDDM, in having a non-Asp residue at position 57 in its chain. To identify the requirements for peptide binding to I-Ag7 and
thereby potentially pathogenic T cell epitopes, we analyzed a known I-Ag7-restricted T cell
epitope, hen egg white lysozyme (HEL) amino acids 9-27. NH2- and COOH-terminal truncations demonstrated that the minimal epitope for activation of the T cell hybridoma 2D12.1 was M12-R21 and the minimum sequence for direct binding to purified I-Ag7 M12-Y20/
K13-R21. Alanine (A) scanning revealed two primary anchors for binding at relative positions
(p) 6 (L) and 9 (Y) in the HEL epitope. The critical role of both anchors was demonstrated by incorporating L and Y in poly(A) backbones at the same relative positions as in the HEL
epitope. Well-tolerated, weakly tolerated, and nontolerated residues were identified by analyzing the binding of peptides containing multiple substitutions at individual positions. Optimally,
p6 was a large, hydrophobic residue (L, I, V, M), whereas p9 was aromatic and hydrophobic (Y
or F) or positively charged (K, R). Specific residues were not tolerated at these and some other
positions. A motif for binding to I-Ag7 deduced from analysis of the model HEL epitope was
present in 27/30 (90%) of peptides reported to be I-Ag7-restricted T cell epitopes or eluted
from I-Ag7. Scanning a set of overlapping peptides encompassing human proinsulin revealed
the motif in 6/6 good binders (sensitivity = 100%) and 4/13 weak or non-binders (specificity = 70%). This motif should facilitate identification of autoantigenic epitopes relevant to the pathogenesis and immunotherapy of IDDM.
Non obese diabetic (NOD)1 mice develop autoimmune, T cell-mediated destruction of pancreatic islet
Although amino acid motifs for peptides that bind to individual class I and some class II MHC molecules have been
well defined (5, 6), the rules that govern binding of peptides to I-Ag7 are still unclear. Reich et al. (7) eluted and sequenced several naturally processed peptides from I-Ag7
and concluded that binding may require an acidic residue
in the COOH terminus of the peptide. Carrasco-Marin et
al. (8) found that I-Ag7 either on the surface of antigen-presenting cells or in SDS-PAGE after its purification was unstable and that the binding of known I-Ag7-restricted T cell
epitopes or the peptides eluted by Reich et al. (7) was difficult or impossible to demonstrate. This led them to hypothesize that weak peptide binding by I-Ag7 militated against
elimination of autoreactive T cells in the NOD mouse.
Amor et al. (9) investigated the fine specificity of peptides
from myelin oligodendrocyte glycoprotein (MOG) or proteolipid protein (PLP) for the induction of experimental allergic encephalomyelitis (EAE) in Biozzi AB/H mice and
suggested a core motif for I-Ag7 binding peptides.
In this study, we used the I-Ag7-restricted T cell epitope,
hen egg white lysozyme (HEL) amino acids 9-27, as a template with which to analyze the amino acid sequence of
peptides that bind to purified, native I-Ag7 and activate a T
cell hybridoma. This has enabled us to define general rules
that identify most known I-Ag7 binding peptides.
Purification of I-Ag7.
I-Ag7 protein was affinity-purified from
detergent lysates of 4G4.7 B cell hybridoma cells by desorption
from OX-6 mouse monoclonal antibody. The 4G4.7 B cell hybridoma was derived by polyethylene glycol (PEG)-induced fusion of
NOD mouse T cell-depleted splenocytes with the HAT-sensitive
A20.2J lymphoma line (10). OX-6 is a mouse monoclonal IgG1
antibody against an invariant determinant of rat Ia, which also recognizes I-Ag7 but not I-Ad (11, 12). Approximately 15 mg of OX-6 antibody was first bound to 4 ml of protein A-Sepharose 4 Fastflow
(Pharmacia, Uppsala, Sweden) and then chemically cross-linked to
the protein A with dimethyl pimelimidate dihydrochloride (Sigma
Chemical Co., St. Louis, MO) in sodium borate buffer, pH 9.0. After 60 min at room temperature (RT), the reaction was quenched
by incubating the Sepharose in 0.2 M ethanolamine, pH 8.0, for
60 min at RT. The suspension was washed thoroughly in PBS
and stored in PBS, 0.02% sodium azide (NaN3).
cells and are a model of human insulin-dependent diabetes mellitus (IDDM) (1). In common with humans who
develop IDDM, NOD mice have immune responses to islet autoantigens such as insulin and glutamic acid decarboxylase (GAD). In addition, they share a structurally similar
class II MHC molecule associated with disease susceptibility. This molecule, I-Ag7, has a
chain sequence otherwise
found only in Biozzi AB/H mice that are susceptible to
chronic relapsing experimental allergic encephalomyelitis
(CR-EAE) (2). It is characterized by a non-Asp residue at
position 57 (3), as in the
chain of the HLA-DQ molecules associated with human IDDM (4). The capacity of
these unique class II molecules to bind and present peptides
to autoreactive T cells could be critical in the development
of IDDM and CR-EAE.
-amino-n-caproic acid and
10 µg/ml each of soybean trypsin inhibitor, antipain, pepstatin,
leupeptin and chymotrypsin. Lysates were cleared of nuclei and
debris by centrifugation at 27,000 g for 30 min and stored as such
if not immediately processed further. To the postnuclear supernatant was added 0.2 vol of 5% sodium deoxycholate (DOC). After
mixing at 4°C for 10 min, the supernatant was centrifuged at
100,000 g at 4°C for 120 min, carefully decanted, and filtered
through a 0.45-µm nylon membrane. The lysate of 5 × 1010
4G4.7 cells was gently mixed overnight at 4°C with 4 ml of OX6-protein A-Sepharose, and the suspension then poured into a
column and washed with at least 50 vol each of buffers A, B, and
C. Buffer A was 0.05 M Tris, pH 8.0, 0.15 M NaCl, 0.5% NP40, 0.5% DOC, 10% glycerol, and 0.03% NaN3; buffer B was
0.05 M Tris, pH 9.0, 0.5 M NaCl, 0.5% NP-40, 0.5% DOC,
10% glycerol, and 0.03% NaN3; buffer C was 2 mM Tris, pH 8.0, 1% octyl-
-D-glucopyranoside (OGP), 10% glycerol, and 0.03%
NaN3. Bound I-Ag7 was eluted with 50 mM diethylamine HCl,
pH 11.5 in 0.15 M NaCl, 1 mM EDTA, 1% OGP, 10% glycerol,
and 0.03% NaN3, and immediately neutralized with 1 M Tris.
Peptide Synthesis.
Peptides were synthesized with a multiple
peptide synthesizer (model 396; Advanced ChemTech, Louisville, KY) using Fmoc chemistry and solid phase synthesis on
Rink Amide resin. All acylation reactions were effected with a
threefold excess of activated Fmoc amino acids, and a standard
coupling time of 20 min was used. Each Fmoc amino acid was
coupled at least twice. Cleavage and side chain deprotection was
achieved by treating the resin with 90% trifluoroacetic acid, 5%
thioanisole, 2.5% phenol, 2.5% water. The indicator peptide for
the binding assay was biotinylated before being cleaved from resin
by coupling two 6-aminocaproic acid spacers on the NH2 terminus and one biotin molecule sequentially, using the above-described procedure. Individual peptides were analyzed by reverse-phase HPLC and those used in this study were routinely 85% pure.
T Cell Hybridoma.
Hybridoma 2D12.1 was generated by PEG-
induced fusion of HEL-immune lymph node cells from a NOD
mouse with the TCR-/
-negative variant of the BW5147 thymoma, as described previously (13). Reactivity of 2D12.1 to HEL
peptides was assayed by incubating 2.5 × 105 NOD spleen cells
and HEL peptides (0.3 nM to 10 µm) with 5 × 104 T hybridoma
cells/well. Culture medium was RPMI 1640 supplemented with
10% FCS, 2 mM L-glutamine, 50 µg/ml gentamicin, and 50 µm 2-mercaptoethanol. After 24 h of culture, 50 µl of supernatants were transferred to culture wells containing 104 IL-2-
responsive CTLL-2 cells. During the final 4 h of a 24-h culture,
CTLL-2 cells were pulsed with 1 µCi [3H]thymidine. Thymidine
incorporation was measured by scintillation spectrometry. The
concentration of peptide that caused 50% of maximum stimulation is referred to as SC50.
I-Ag7 Peptide-binding Assay. Peptides were dissolved at 10 mM in DMSO and diluted into 20% DMSO/PBS for assay. Indicator I-Ag7 binding peptide, HEL 10-23, was synthesized with a biotin molecule and two spacer residues at the NH2 terminus. Approximately 200 nM of this biotinylated HEL peptide and each test peptide in seven concentrations ranging from 50 µM to 50 pM, were coincubated with ~200 ng of I-Ag7 protein in U-bottomed polypropylene 96-well plates (Costar Serocluster, Costar Corp., Cambridge, MA) in binding buffer at RT. The binding buffer was 6.7 mM citric phosphate, pH 7.0, with 0.15 M NaCl, 2% NP-40, 2 mM EDTA, and the protease inhibitors as used in the lysis buffer. After a minimum of 24 h, each incubate was transferred to the corresponding well of an ELISA plate (Nunc Maxisorp, Nunc, Roskilde, Denmark) containing prebound OX-6 antibody (5 µg/ml overnight at 4°C, followed by washing). After incubation at RT for at least 2 h, and washing, bound biotinylated peptide-I-Ag7 complexes were detected colorimetrically at 405 nm after reaction with streptavidin-alkaline phosphatase and paranitrophenolphosphate. Competition binding curves were plotted and the affinity of peptide for I-Ag7 was expressed as an inhibitory concentration 50 (IC50), the concentration of peptide required to inhibit the binding of bio-HEL 10-23 by 50%.
Approximately 2 mg
of protein, estimated by Coomassie blue binding (Bio Rad
Protein assay), was purified from 5 × 1010 4G4.7 cells. In
SDS-PAGE, the majority (>95%) of the protein was resolved as two bands of molecular weight ~33,000 and
~28,000 that correspond to the and
subunits, respectively, of mouse class II MHC molecules (data not shown).
The competition binding assay with purified I-Ag7 was sensitive and specific (Fig. 1), and highly reproducible; in 15 separate assays the mean ± SD of the IC50 for competition between biotinylated and unlabeled HEL 10-23 was 295 ± 72 nM.
Carrasco-Marin et al. (8) were unable to demonstrate direct binding of HEL 11-25 to purified I-Ag7 and proposed
that I-Ag7 was inherently unstable. We found that purified
I-Ag7 stored at 70°C for more than 1 yr reproducibly
bound HEL 10-23 with high affinity. Therefore, our results do not support their hypothesis that I-Ag7 is inherently
unstable, which they postulated would impair its ability to
bind and induce tolerance to autoreactive peptides.
Peptides representing sequential truncations of HEL 9-27, from either the NH2 or COOH-terminus, were each assayed in parallel for binding to I-Ag7 and for their ability to activate the 2D12.1 hybridoma. Inspection of these data (Table 1) reveals that the minimum T cell epitope is M12-R21, and the minimum binder is M12-Y20 or K13-R21.
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Substitution of alanine (A) at each position in HEL 12-22 (Table 2) had no significant effect on binding, with the sole exceptions of positions L17 and Y20. Substitution at either of these two positions virtually abolished binding. On the other hand, while having no effect on binding, substitutions by A at K13, R14, H15, G16, and D18, and to a lesser extent at R21, abolished T cell activation. Removal of R21 (see Table 1) abolished T cell activation. Further substitutions of representative amino acids (D, K, P, Y, L, Q) at each position (Table 2) revealed varying levels of tolerance of specific residues/positions for binding (see below) and generally confirmed the results of the alanine substitutions on T cell activation. On the basis of these results, we can deduce that most residues in the minimal T cell epitope HEL 12-21 have TCR contacts and that two, L17 and Y20, are essential for binding to I-Ag7 (Fig. 2).
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Anchor Residues for Peptide Binding.
The critical roles of L17 and Y20 in the HEL epitope, as model anchor residues for binding to I-Ag7, was demonstrated with poly(A) peptides (Table 3). The nonbinding poly(A) peptide, KAA AAAAAA, was converted to a super binder simply by incorporating L and Y at the same relative positions as in the HEL epitope. Either residue alone was not sufficient. Binding was reconstituted only when these two residues were appropriately spaced and in the correct order. In addition, this approach reveals the importance of the frame or context of the anchor residues. The LAAY sequence must be flanked by at least two As, an absence of which at the COOH terminus can be compensated for by at least three As on the NH2 terminus, but not vice versa. This suggests that binding of these specific residues within the I-Ag7 groove requires stabilization by hydrogen bonding from nonspecific flanking residues, in particular at the NH2 terminus. For the purpose of further analysis, the relative positions (p) of L and Y in the HEL epitope 12-21 are designated p6 and p9.
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In addition to
the selected substitutions at all positions (see Table 2), we
investigated the effect on binding of all possible substitutions (except labile cysteine) at p6 or p9. A single residue substitution was classified as well tolerated, weakly tolerated, or nontolerated according to a threshold on its IC50
value: well tolerated, <1,000 nM; weakly tolerated, 1,000-
10,000 nM; nontolerated, 10,000 nM. Although somewhat arbitrary, this classification corresponds to generally
accepted notions of good binders, moderate binders, and
weak to non-binders. The results, combined with those
from Table 2, are presented in Table 4. Optimally, p6 is a
large, hydrophobic residue (L, I, M, V), whereas p9 is aromatic and hydrophobic (Y, F) or positively charged (K, R).
Most amino acids are not well tolerated at these anchor positions. Additionally, specific amino acids are not tolerated
at other positions, namely F and E at p3 and W and Y at
p8. This information allowed us to propose and test minimum
rules for a motif for I-Ag7 binding peptides. These were that
a binder must have the following: (a) two well-tolerated residues or one well-tolerated and one weakly-tolerated residue
at anchor positions p6 and p9, (b) no nontolerated residues
at positions p3 and p8, and (c) at least two residues flanking
p6 and p9, or at least three residues NH2-terminal of p6.
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Relatively few peptides containing sequences that might bind to I-Ag7 have been reported in the literature. They include peptides that stimulate I-Ag7-restricted T cells or T cell hybridomas, compete for antigen presentation to T cell hybridomas, induce EAE in Biozzi AB/H mice, or have been eluted from I-Ag7 (listed in Table 5). It should be noted that apart from the present study and that of CarrascoMarin et al. (8), binding has not been determined by direct peptide interaction with purified I-Ag7, but either by elution from I-Ag7, competition with peptides that activate I-Ag7- bearing T cell hybridomas or induction of EAE. The motif we have defined correctly identifies 27/30 (90%) of the published sequences (Table 5). Interestingly, we found that one of these sequences, mouse serum albumin 560-574, that does not contain the motif, did not bind to I-Ag7 (data not shown).
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Two groups have suggested putative motifs for peptides that bind to I-Ag7. Reich et al. (7) found that several peptides eluted from I-Ag7 had an acidic residue at the COOH terminus. Their data also indicated that this residue was separated by three from a basic residue. Whereas basic residues are major p9 anchors in our motif, an acidic residue at the COOH terminus is not a uniform feature of other peptides deduced (Table 5) or shown (Table 6) to bind to I-Ag7. However, it is conceivable that in some cases, e.g., OVA 323-339 (see Table 5), a COOH-terminal acidic residue could compensate for a nontolerated residue at p9. Amor et al. (9) described a possible motif shared by encephalitogenic peptides in the Biozzi AB/H (I-Ag7) mouse. It contained hydrophobic (I or L), basic (K, R, or H), a small T cell contact (A or G) and large hydrophobic (L or F) residues within a 6- to 7-amino acid core. They studied the effect of K substitutions on the immunogenicity of phospholipid protein 56-70 (see Table 5), in which they had deduced a core sequence, NVIHAFQ, necessary for the induction of EAE. This sequence contains our motif I (p6) and F (p9). K substitutions at I, H, A, or F completely abolished the ability of the peptide to induce EAE. We would have predicted abolition of binding by the K substitution at I or A and, by analogy with HEL (see Table 2), a significant reduction in T cell activation by K substitution at H or F. Thus, the features of this encephalitogenic motif are contained within the expanded and generalized motif we have described.
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We tested peptides overlapping by four residues and spanning the entire sequence of human proinsulin for binding to I-Ag7, and inspected them for presence of the binding motif (Table 6). All six (100%) good binders contained a motif. However, a motif was present in 4/13 (30%) weak or non-binders. Clearly, the motif rules do not fully account for the effects of residue combinations or flanking sequences. Proinsulin 5-19 has a well-tolerated V at p6 and a weakly tolerated L at p9 yet did not bind, but when this anchor pair moves towards the NH2 terminus in the following 9-23 sequence, the peptide becomes a binder. Proinsulin 45-59 has a well-tolerated L at p6 and a weakly tolerated L at p9 and binds with high affinity, but when this anchor pair moves towards the NH2 terminus in the following 49-63 sequence, the affinity of the peptide decreases. Human proinsulin 17-31 has a weakly tolerated Y at p6 and a well-tolerated K at p9, yet does not bind. Although this anchor pair is close to the COOH terminus, this does not preclude other peptides, e.g., human proinsulin 65-79, from binding with high affinity. However, human proinsulin 17-31 has a positively charged p9/COOH terminus, whereas the other binding peptides are generally neutral or tend to be acidic. Reich et al. (7) noted a bias towards acidic residues at the COOH termini of peptides they eluted from I-Ag7. When the anchor pair in this peptide moves towards the NH2 terminus in the following 21- 35 sequence with an acidic COOH terminus, the peptide becomes a binder. Thus, in a small set of unbiased peptides, the motif appears to have high sensitivity and some degree of specificity. A similar degree of specificity was found for an I-Ek motif by correlating binding with the presence of the motif in a panel of ~150 peptides (23).
Although the core region length of class II MHC binding peptides is ~13 residues (24), analysis of binding motifs (25) indicates that only nine residues within the core region are essential for binding. The motif we describe conforms with this requirement. It is simplistic in the sense that each residue is assumed to contribute to binding independently of other residues and, when located at a given position, to contribute the same amount to binding even within different sequences. The rules that govern binding to class II MHC molecules are more complex and will have to take account of interactions between residues. Nevertheless, by defining tolerated and nontolerated residues for binding at key positions in HEL 12-22, we appear to have unearthed general rules that identify a large majority of known binders to I-Ag7, and discriminate most non-binders.
The high sensitivity of the motif for reported I-Ag7 binders or T cell epitopes is remarkable, but the utility of the motif will depend on its specificity, i.e., its absence in nonbinders. Specificity was 70% for the peptides in Table 6, but the database is small. Even if somewhat degenerate in its present form, the motif should considerably narrow the search for possible binders. The I-Ag7 binding assay we have described is robust and will enable the database of binders and non-binders to be enlarged progressively to further validate the motif. Experiments to fine tune the motif by using peptide libraries are in progress. Just as a motif for human class II DR4 (*0401) binding peptides was applied to scan candidate autoantigen proteins in rheumatoid arthritis for potential epitopes (26), so also might the motif for I-Ag7 binding be applied to identify potential autoepitopes for IDDM in NOD mice and CR-EAE in Biozzi AB/H mice.
Address correspondence to L.C. Harrison, The Walter and Eliza Hall Institute of Medical Research, Post Office, Royal Melbourne Hospital, Parkville, 3050, Australia.
Received for publication 18 November 1996 and in revised form 8 January 1997.
L.C. Harrison and M.C. Honeyman were supported by the National Health and Medical Research Council of Australia and a Diabetes Interdisciplinary Research Program grant from the Juvenile Diabetes Foundation International. P. Augstein was supported by Deutscher Akademischer Austauschdienst, Bonn. Secretarial assistance was provided by Margaret Thompson.1. | Kikutani, H., and S. Makino. 1992. The murine autoimmune diabetes model: NOD and related strains. Adv. Immunol. 51: 285-322 [Medline]. |
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