Proliferation Kinetics Associated with T Cell Receptor-beta Chain Selection of Fetal Murine Thymocytes

By Ingrid Falk, Judit Biro, Hubertus Kohler, and Klaus Eichmann

From the Max-Planck-Institut für Immunbiologie, 79108 Freiburg, Germany

Summary
Materials and Methods
Results
Discussion
Footnotes
References


Summary

After productive rearrangement of a TCRbeta chain gene, CD4-8- double negative (DN) thymocytes express TCRbeta polypeptide chains on the cell surface together with pre-Talpha and the CD3 complex forming the pre-TCR. Signals transmitted through the pre-TCR select TCRbeta + DN thymocytes for further maturation to the CD4+8+ double positive stage, whereas DN cells that fail to generate a productive TCRbeta gene rearrangement do not continue in development. This process is termed TCRbeta chain selection. Although it is likely that differences between proliferation dynamics of TCRbeta + and TCRbeta - cells may play a role, the exact mechanisms of TCRbeta chain selection have not been elucidated. We therefore studied the proliferation dynamics of TCRbeta + and TCRbeta - thymocytes during fetal development, i.e., when TCRbeta chain selection takes place for the first time. We analyzed in situ accumulation of TCRbeta + thymocytes by confocal microscopy, and determined cell cycle and division parameters of TCRbeta + and TCRbeta - populations by flow cytometry. About 600 TCRbeta + cells/thymic lobe are generated by independent induction events between days of gestation (dg) 13.5. and 15.5. As of dg 14.5, most TCRbeta + cells have entered S/G2 phase of cell cycle, followed by seven to eight rapid cell divisions in fetal thymic organ culture, suggesting a corresponding burst of nine cell divisions within 4 d in vivo. By dg 18.5, the division rate of TCRbeta + cells has slowed down to less than 1/d. About three quarters of TCRbeta - cells divide at a slow rate of 1/d on dg 14.5, the proportion of nondividing cells increasing to 50% within the following four d. From dg 16.5 onwards, TCRbeta - cells, but not TCRbeta + cells, contain a significant proportion of apoptotic cells. The results suggest that failure to become selected results in shutdown of proliferation and eventual programmed cell death of fetal TCRbeta - cells. Positive selection of fetal TCRbeta + cells is achieved by an increased rate of cell divisions lasting for approximately 4 d.


During development in the thymus, alpha beta lineage thymocytes proceed through two main developmental checkpoints. First, cells with a CD4/CD8 double negative (DN)1 phenotype differentiate to CD4/CD8 double positive (DP) cells. Second, DP thymocytes develop into CD4 or CD8 single positive (SP) cells (reviewed in reference 1). Progression through either of these checkpoints is the result of a process of selection (reviewed in reference 2). Differentiation of DN to DP cells depends on the expression of the pre-TCR, consisting of the TCRbeta chain, pre-Talpha (3, 4), and components of the CD3 complex (reviewed in 5). Differentiation of DP to SP cells depends on the expression of a mature TCR, consisting of the TCRalpha beta heterodimer and the CD3 complex (2, 10). The TCRbeta genes rearrange during the DN stage (11), and expression of the preTCR selects for the approximately 56% of rearranging cells that statistically generate a productive TCRbeta gene (14). The TCRalpha genes rearrange during the DP stage (15). Thus, selection by the pre-TCR, also termed TCRbeta chain selection, generates the repertoire of TCRbeta chains to be combined with TCRalpha chains in the DP stage. A putative ligand for the selection by the pre-TCR is not known (16). DP cells expressing the mature alpha beta TCR are selected, positively or negatively, by the ability of the alpha beta TCR to interact with MHC/peptide ligands, thus generating the self tolerant, self-MHC restricted repertoire of CD4 or CD8 SP thymocytes and peripheral T cells (17, 18)

Pre-TCR-induced maturation of DN to DP thymocytes is associated with a complex cluster of differentiation events (reviewed in reference 8), including allelic exclusion of the TCRbeta locus (10, 19, 20), initiation of germline transcription of the TCRalpha locus (20, 21), transient downregulation of the expression of the RAG genes (20, 22), shutdown of CD25 (23), and transient expression of the activation marker CD69 (26). Moreover, a large number of DP thymocytes is generated from a small number of DN cells, presumably by induction of cell proliferation. This expansion phase is impaired in the absence of TCRbeta (27) or of pre-Talpha (33). However, TCRbeta -deficient DN thymocytes can be induced to proliferate and to differentiate to DP cells by cross-linking CD3epsilon , expressed on DN thymocytes before and independent of TCRbeta (23, 34). These results suggested that cell proliferation associated with the differentiation of DN to DP thymocytes may be induced by pre-TCR-mediated signal transduction (26).

Cell division parameters of immature thymocytes have previously been studied in adult mice. Shortman et al. (35) studied proliferation and turnover of DN thymocytes by thymidine labeling in vivo and propidium iodide (PI) staining, and suggested the following sequence: the dividing, most immature DN CD44+ (PgP-1+) CD25- (IL-2Ralpha -) thymocytes mature sequentially through a nondividing CD25+CD44- stage into dividing CD25-CD44- DN cells before they enter the DP stage and exit cell cycle. Most of TCRbeta rearrangement has been subsequently shown to take place at the nondividing CD25+CD44- transitory stage (12, 13). More recently, Penit et al. (36) reported results from 5-bromo-2'-deoxyuridine (BrdU) labeling of thymocytes in vivo suggesting that 70% of the CD25+CD44- DN cells were resting while 30% were proliferating. Hoffman et al. (37) showed that a subset of CD25+CD44- DN thymocytes expressed markers of cell division, such as CDK2 and cdc2. Since proliferating CD25+CD44- cells were not seen in RAG-deficient mice (36, 37), these authors speculated that the proliferating cells might be those that had produced functional TCRbeta genes. However, none of these studies directly addressed the relationship between TCRbeta expression and thymocyte proliferation. Parkin et al. (38) observed mitotic activity in newborn DN TCRbeta + thymocytes, but proliferation kinetics of TCRbeta - cells have not been determined. Moreover, cell cycle and division parameters in fetal thymic development have not been studied.

The thymic anlage of mice starts to become repopulated with thymocyte precursors on day of gestation (dg) 11-12. TCRbeta gene VDJ rearrangements were first detected in fetal thymocytes on dg 15.5 (39, 40). Cells expressing TCRbeta chains were also observed on dg 15.5 by immunohistochemistry (41) and flow cytometry (FCM) among CD25+CD44- thymocytes (42). In the present report, we studied the accumulation and cell cycle dynamics of TCRbeta + thymocytes in ex vivo fetal thymuses and in dg 14.5 fetal thymic organ cultures (FTOC) during the following four d in vitro, using confocal laser scanning microscopy (CLSM) and flow cytometric analyses of PI- and BrdU-labeled cells. We detect TCRbeta + cells from dg 13.5 onwards. Before dg 15.5, TCRbeta + cells appear to accumulate mostly by independent induction events to reach approximately 600 independent TCRbeta + cells/thymic lobe at dg 15.5. Concomitantly, TCRbeta + cells rapidly enter the S/G2 phase of cell cycle. In FTOC, this is followed by 3 d of rapid proliferation, comprising at least seven to eight cell divisions, and an increase in total TCRbeta + cells of almost 200-fold. In vivo data suggest a corresponding burst of up to nine cell divisions and an approximately 500-fold expansion between dg 14.5 and 18.5. Thereafter, TCRbeta + cells return to slow cycle conditions. A majority of TCRbeta - cells appears to be slowly cycling on dg 14.5 with a progressive increase in the proportion of noncycling TCRbeta - cells to 50%, resulting in a 8-(in vitro) to 14-(in vivo) fold overall increase in TCRbeta - cells during the 4 d of observation. A significant proportion of apoptotic cells is seen from dg 16.5 onwards among TCRbeta -, but not among TCRbeta + thymocytes.


Materials and Methods

Mice. BALB/c mice were obtained from our own specific pathogen-free animal facility. For timed pregnancies, mice were allowed to mate overnight (5 PM to 7 AM) and screened for vaginal plug. Noon of the following day was defined as dg 0.5. For in vivo labeling, pregnant mice were injected 18 h before analyses of fetal thymocytes with 250 µg of BrdU (Boehringer Mannheim GmbH, Mannheim, Germany), and drinking water was supplemented with 1 mg/ml of BrdU.

Fetal Thymic Organ Culture. Fetal thymic organ cultures were performed with dg 13.5 or 14.5 thymic lobes as previously described (23, 24). Cultures were carried out for 1-4 d, referred to as day +1, +2, +3, and +4, respectively. BrdU labeling was done by incubation for 18 h with 10 µM concentration before analysis.

mAb. The following mAb, labeled with FITC or PE, were purchased from PharMingen (San Diego, CA): anti-CD44, clone IMF; anti-TCRbeta , clone H57-597; anti-TCRVbeta 6, clone RR4-7; and anti-TCRVbeta 3, clone KJ25. Anti-CD25, clone 5A2, was labeled with FITC in our own laboratory. FITC labeled anti-BrdU clone BMC9318 was from Boehringer Mannheim, GmbH.

FCM. For propidium iodide/intracellular (IC) TCRbeta double staining cells were first incubated with culture supernatant of mAb 2.4G2 to block FCgamma RII/III. Cells were then fixed with 1% paraformaldehyde for 30 min on ice, followed by three washing steps in PBS/2% FCS, the last containing, in addition, 0.1% saponin (Lot No. P4170; Sigma Chemical Co., Heidelberg, Germany). FITC labeled anti-TCRbeta mAb in PBS/0.125% saponin was added for 20 min on ice, followed by two quick washes with PBS and 2 × 20 min on a rocking platform in PBS/2% FCS/ 0.1% saponin at 4°C. Cells were then fixed again in 1% paraformaldehyde, washed, incubated for 30 min at 37°C with 100 µg/ml RNAse (Boehringer Mannheim, GmbH) and stained with 20 µg/ml propidium iodide (Sigma Chemical Co.). After washing, cells were analyzed by FCM on a FACScan® (Becton Dickinson, Mountain View, CA) using gates (signal area versus signal width) that exclude doublet signals.

BrdU-labeled cells were first stained for intracellular TCRbeta as described above, and were sorted into TCRbeta + and TCRbeta - populations using a FACStar® plus (Becton Dickinson). Cells were then fixed with 70% ethanol for 30 min on ice, resuspended in 0.1 M HCl, 0.5% Triton X-100 for 10 min on ice, centrifuged, and resuspended in H2O. DNA was denatured by heating for 10 min at 95°C (43) followed by rapid cooling with ice-cold H2O. This heating step obliterated all TCRbeta fluorescence. After one wash in 0.5% Triton X-100 in PBS, cells were incubated with FITC-labeled anti-BrdU mAb for 30 min at RT. FCM was done using a FACScan®.

Triple analyses for TCRbeta , BrdU incorporation, and PI staining first used a sort for intracellular TCRbeta , followed by staining for BrdU incorporation, as described above. PI staining was done last, also as described above.

Confocal Laser Scanning Microscopy. Whole thymic lobes were quickly rinsed three times in PBS and then fixed with 2% paraformaldehyde for 3 h at 4°C on a rocking platform. They were washed twice in PBS, twice in PBS/1% FCS/1% Tween-100, and then incubated for 7 min in acetone at -20°C. This was followed by resuspension in H2O and 2 × 5 min rocking in PBS/ FCS/Tween. For mAb staining fixed lobes were first incubated for 30 min with unlabeled mAb 2.4 G2 and then for 30 min with each fluorochrome-labeled mAb alternating with 2 × 30 min rocking in PBS/FCS/Tween, all at 37°C. Lobes were then embedded on microscope slides in Fluoromount-C (Southern Biotechnology Assoc., Birmingham, AL), covered by a cover slip, turned upside down, and analyzed by an inverted CLSM instrument (Leica Instrs. GmbH, Heidelberg, Germany). Lobes were minimally compressed by this technique. Magnification was 400fold if not otherwise stated.

PCR for TCRbeta rearrangement. DNA was prepared from thymocytes as described (39). DNA was amplified in 25-µl reaction buffer, containing 0.1 µg DNA, 0.5 µM 5' and 3' primers, 200 µM of each dNTP (Pharmacia, Freiburg, Germany) and 0.2 U SuperTaq (Staehelin, Basel, Switzerland) for 34 cycles (40 s at 94°C, 60 s at 63°C, 120 s at 72°C). Primers were 5'Vbeta 3 (44), 5'Vbeta 8, 5'Dbeta 2, and 3'Jbeta 2 (45).


Results

Kinetics of Accumulation of the TCRbeta + and TCRbeta - Subsets In Vivo and in FTOC.

Fetal thymuses were obtained from pregnant BALB/c mice from dg 14.5 to 18.5 and FTOC, were set up using dg 14.5 thymic lobes cultured for one to four d. Thymocytes were stained on each day for IC TCRbeta chain, and Table 1 gives the absolute numbers of TCRbeta + and TCRbeta - cells determined in both sets of experiments. About 100 TCRbeta + cells/lobe were detected on dg 14.5. This is not a staining artefact, as TCRbeta + cells identified by FCM on dg 14.5 differ drastically in PI staining pattern from TCRbeta - cells (see Fig. 5). Moreover, we have no difficulties in demonstrating VDJ rearrangements by PCR in dg 14.5 thymic lobes (see below, Fig. 7 C). In vivo, numbers of TCRbeta + cells increased about 10-fold each day until dg 17.5, at which time the majority turned into DP thymocytes (data not shown; 42). This was followed by a 3-4-fold increase of TCRbeta + cells until dg 18.5. TCRbeta - cells increased merely 14-fold over the entire time interval. In vitro, numbers of TCRbeta + cells increased fourfold during the first day of culture, and then eight-ninefold on each of the next 2 d and two-threefold on day four. TCRbeta - thymocytes increased about eightfold until day +4 in FTOC. The end result is a proportion of TCRbeta + cells of ~80% by day 18.5 in vivo or of ~50% after 4 d of FTOC. The comparison between the cell increments in vitro and in vivo reveals the main difference between dg 15.5 and day +1 of culture, presumably reflecting a lag due to the recovery of the thymic lobes from the handling procedures. The differences later in the time course are smaller and may to some extent be due to lack of influx of new cells in vitro.

Table 1. Accumulation of TCRbeta + and TCRbeta - Cells in Early Fetal Thymic Development*


In Vivo In FTOC
TCRbeta + TCRbeta - TCRbeta + TCRbeta -

dg 14.5 ~102 1.1 × 104
dg 15.5 1.22 × 103 (1.22) 2.9 × 104 day+1  0.45 × 103 (1.8) 1.5 × 104
dg 16.5 1.12 × 104 (1.18) 4.9 × 104       +2  4.1 × 103 (1.67) 2.8 × 104
dg 17.5  1.4 × 105 (1.32) 7.5 × 104       +3  3.2 × 104 (1.48) 5.7 × 104
dg 18.5  5.5 × 105 (1.41) 1.5 × 105       +4  7.9 × 104 (1.62) 8.5 × 104

*  Absolute numbers of thymocytes/thymic lobe estimated from the percentages of TCRbeta IC+ cells in FCM. Standard errors of the geometric means of several experiments in parentheses.


Fig. 5. Cell cycle analysis by PI staining of TCRbeta + and TCRbeta - thymocytes from fetal thymic lobes recovered from BALB/c fetuses at dg 14.5, and from dg 14.5 FTOC cultured for 1-4 d. Thymocytes were double stained for intracellular TCRbeta (TCRbeta IC, top) and PI. The lower panels show the PI staining (shaded patterns) of cells gated positively or negatively for TCRbeta . Percentages of TCRbeta + cells and of cells with hypodiploid, diploid, and hyperdiploid amounts of DNA are given in each frame. LC, percentage of large blastoid cells as determined by scatter parameters.
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Fig. 7. (A) BrdU incorporation into fetal thymic lobes recovered from BALB/c fetuses on dg 13.5 and kept in organ culture for 1 d. Experimental procedure and data representation essentially as in Fig. 6, except that the negative controls for BrdU staining are unmarked. (B) Triple analysis for TCRbeta , BrdU incorporation, and PI staining of thymocytes recovered from dg 15.5 and 17.5 fetal thymic lobes of pregnant BALB/c mothers injected 18 h earlier with BrdU. IC TCRbeta staining and sorting not shown. Sorted TCRbeta + and TCRbeta - cells were double stained with PI (top, shaded patterns) and for BrdU incorporation (bottom). BrdU incorporation is shown separately for cells with diploid (horizontal stripes) and hyperdiploid (vertical stripes) PI staining. The small difference in BrdU intensity between the two populations is not significant, as there is some spillover of PI fluorescence into the FITC chanel. Negative controls (unmarked patterns) are as described in Fig. 6. (C) Analysis of TCRbeta locus DJ and VDJ rearrangements by PCR in dg 14.5 fetal thymocytes (14.5), and in adult thymus (AT). Ethidium bromide-stained gel shown, inversely photographed. The six fragments seen with 5'Vbeta 8 and 5'Vbeta 3 primers correspond, from top to bottom, from VDbeta 2Jbeta 2.1 to VDbeta 2Jbeta 2.6 rearrangements. The additional fragment at the top of the Dbeta 2 to Jbeta 2 rearrangements represents the germline configuration.
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Fig. 6. BrdU incorporation in fetal thymic lobes recovered from BALB/c fetuses on dg 14.5 and kept in organ culture for 1-4 d. BrdU was added to the culture medium 18 h before termination of culture. Thymocytes were stained for TCRbeta (upper panels, percentages of TCRbeta + cells indicated), sorted into TCRbeta - and TCRbeta + cells (second panels, cross-hatched and dotted patterns, respectively), and analyzed for BrdU incorporation (third and fourth panels). Note the three levels of BrdU staining (vertical stripes). Percentages of BrdU negative cells and of partially and completely BrdU-labeled cells indicated in each frame. Negative controls (shaded) used thymocytes recovered from thymic lobes cultured without BrdU, and then incubated with BrdU for 30 min on ice, washed, and subjected to the antiBrdU staining procedure.
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Subset Relationships of Early Fetal TCRbeta + Thymocytes.

Expression of intracellular TCRbeta chains before dg 15.5 has not been previously described and it was therefore nessessary to determine the subset relationships of these early TCRbeta + thymocytes. To this end, we prepared fetal thymic lobes from BALB/c mice at dg 13.5 and 14.5 and analyzed them by CLSM for TCRbeta and CD44. Fig. 1 shows that TCRbeta + cells are detected as early as dg 13.5. However, even at this early timepoint, all unequivocally identified TCRbeta + cells were negative for CD44. In a second experiment (Fig. 2), fetal thymic lobes were prepared on dg 14.5, 15.5, and 16.5, and analyzed by CLSM for TCRbeta and CD25. Out of 25 individually studied TCRbeta + cells on dg 14.5, 14 were were weakly positive (upper panel), and 11 were negative for CD25 (second panel). On dg 15.5 and 16.5, CD25 staining changed from a homogeneous to a spotty pattern, and the proportion of CD25+TCRbeta + cells decreased, in agreement with previous results from FCM (42). Together, these results suggest that even at this early stage in development, TCRbeta expression does not preceed the CD25+CD44- stage of DN thymocytes.


Fig. 1. Confocal laser scanning microscopy of fetal thymic lobes recovered from BALB/c fetuses on dg 13.5 and 14.5. Two examples of dg 14.5 are shown. Left, Staining with FITC labeled anti-CD44; right, PE-labeled anti-panTCRbeta ; center, two color representation. Horizontal side length of one field is 100 µm.
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Fig. 2. Confocal laser scanning microscopy of fetal thymic lobes recovered from BALB/c fetuses on dg 14.5, 15.5, and 16.5. Two examples of dg 14.5 are shown. Left, staining with FITC-labeled mAb to CD25; right, PE-labeled anti-panTCRbeta mAb; center, two color representation. Note that the TCRbeta + cell in the upper example of dg 14.5 is CD25low, and in the lower example is CD25-. The side length of one square field is 100 µm.
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Morphodynamics of the Accumulation of TCRbeta + Thymocytes In Vivo and in FTOC.

As shown in Fig. 2, TCRbeta + cells were readily detected on dg 14.5, and increased in density until dg 16.5, in parallel with a decrease in CD25+ cells. TCRbeta + cells on dg 14.5 are dispersed as rare individual cells whereas on dg 15.5 some of them appear as doublets or as large dividing cells, indicating that cell division may have started.

Ex vivo thymic lobes later than dg 16.5 were not readily analyzed by CLSM. Therefore, FTOC were set up with dg 14.5 thymic lobes and cultured for an additional 4 d. Individual lobes were fixed on each day of culture and double stained with mAbs against CD25 and against TCRbeta (Fig. 3). The density of TCRbeta + cells after 1 d of culture was higher than on dg 14.5, but appeared lower than on dg 15.5 in ex vivo thymic lobes (see Fig. 2), and most TCRbeta + cells were still dispersed individually with doublets rarely seen. On each of the following 2 d of culture, an increase in the number of TCRbeta + cells was seen, with local accumulations within the field of observation on day +3. A further increase in TCRbeta + cells was seen on day +4, with the cells distributed densely over the entire field of observation. The intensity of CD25 staining and the number of CD25+ cells decreased in parallel with the increase in TCRbeta + cells, slightly delayed compared to the ex vivo analyses.


Fig. 3. Confocal laser scanning microscopy of fetal thymic lobes recovered from BALB/c fetuses at dg 14.5 and kept in organ culture for 1-4 d. Left, FITC-labeled anti-CD25; right, PE-labeled anti-panTCRbeta ; center, two color representation. The side length of one square field is 100 µm.
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The accumulation of TCRbeta + cells in FTOC was also studied using mAb to individual TCR Vbeta gene products. Fig. 4 shows analyses using mAb to Vbeta 3 and Vbeta 6, which gave the best signal to noise ratios. For both TCR Vbeta gene products, rare individual cells and a few doublets could be detected on day +1 and cellular clusters were detected from day +2 onwards. These clusters are interpreted as dividing TCRbeta clones. For unkown reasons, cluster morphology differed between clones expressing TCRVbeta 3 and TCRVbeta 6, the former showing tightly packed and the latter showing more loosely associated accumulations of cells. Attempts were made to estimate cluster sizes by a combination of counting cells in individual scans taken at 3 µ intervals, and by superimposing 3 µ scans from top to bottom through a cluster. The results are compiled in Table 2 and suggest that clusters on day +2 range from 4 to 14 cells with clusters of 6 to 8 cells predominating. On day +3 estimates were more difficult, suggesting an average of 30 to 40 cells/cluster. Estimates of cluster sizes on day +4 were not possible, but a further increase in cluster size was apparent (not shown). These data suggest that individual TCRbeta + cells begin to proliferate in FTOC during day +1 and run through at least five cell divisions within the following 2 d, with additional cell divisions thereafter.


Fig. 4. Confocal laser scanning microscopy of fetal thymic lobes obtained from BALB/c fetuses on dg 14.5 and kept in organ culture for 1-3 d. PE-labeled anti-Vbeta 3 and anti-Vbeta 6 mAb used for staining, as indicated. For each cluster, a single optical section is shown. The total number of cells/cluster is usually considerably larger than the number of cells seen in the sections shown ( see Table 2).
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Table 2. Sizes of TCRbeta Clusters in FTOC*


Day of culture
+1 +2 +3

Vbeta 3 11 × 1,2,2,3 4,5,6,6,9,11,14 23,24,32,33,42
(1.3) (7.8) (31)
Vbeta 6 14 × 1,2,3 5,6,6,8Dagger 29,34,37,45,46
(1.2) (6.25) (38.2)

*  Cluster sizes determined by confocal laser scanning microscopy by scanning optical sections at 3 µm intervals. For examples see Fig. 4.
Dagger  Due to the loose association of Vbeta 6+ cells, only a few safe examples of clusters could be analyzed.

Cell Cycle Analysis by Propidine Iodide Staining.

FTOC were used to focus the cell cycle analyses on a population that entered the thymus within a limited time window and was therefore expected to develop in a quasi-synchronous fashion. To this end, FTOC were set up on dg 14.5, and cells were double stained with anti-TCRbeta mAb and PI immediately and on each of 4 d of culture. As shown in Fig. 5, TCRbeta + cells contain high proportions of cells with hyperdiploid concentrations of DNA, characteristic of the S/G2 phases of the cell cycle. The proportion of TCRbeta + cells in S/G2 is highest on dg 14.5, and decreases only slightly until day +4. This suggests that virtually all TCRbeta + cells are in cell cycle for the entire period of observation. The percentages of cells in S/G2 among TCRbeta - cells are considerably lower, and decrease from ~30% on dg 14.5 to <10% on day +4 of culture. Nevertheless, these data show that on dg 14.5 and during the initial days of culture, the TCRbeta - subset contains a significant proportion of cycling cells as well. Proportions of blastoid cells were in good agreement with cells in S/G2 in all cases. Note, from day +2 onwards, small proportions of cells with hypodiploid DNA content are seen among the TCRbeta -, but not among the TCRbeta + population. The detection of hypodiploid cells in only one of the two subsets argues against a tissue culture artefact (also see below) and suggests that TCRbeta - cells may eventually die by apoptosis.

Proliferation Kinetics by Labeling with BrdU.

For labeling with BrdU, we sorted TCRbeta + and TCRbeta - cells from FTOC preincubated with BrdU, and stained them with anti-BrdU mAb after denaturation of the DNA by heating to 90° (43). This method reliably permitted the differentiation of two distinct levels of BrdU incorporation, apparently corresponding to partial and saturating DNA labeling. Since the results from CLSM suggested that TCRbeta + fetal thymocytes divide at least 2-3 times/day between days +1 and +3 of FTOC, we chose a labeling period of 18 h before analysis. During this time interval, nearly all asynchronously dividing cells with a cycle time of less than nine h should have acquired a saturating BrdU label, whereas longer cycle times should have been revealed by increasing proportions of partially labeled and unlabeled cells. The results of representative experiments for each day are compiled in Fig. 6. TCRbeta + cells on day +1 fell into three categories: one third remained unlabeled, one third incorporated a partial, and one third incorporated a saturating dose of BrdU. On day +2, the vast majority of TCRbeta + cells had incorporated a saturating dose, whereas this proportion dropped to ~50% on day +3. On day +4, nearly all of the TCRbeta + cells showed partial BrdU labeling. About 20-25% of the TCRbeta - cells remained unlabeled from day +1 to d +3, whereas the rest of the cells incorporated a partial BrdU label. By day +4, the proportion of unlabeled TCRbeta - cells increased to 50%. Together, these results suggest that TCRbeta + cells enter a phase of rapid proliferation on day +1 of FTOC with some of the cells going through at least one division. Essentially all of the cells progress through at least three divisions on day +2, and most of the cells through another three divisions on day +3. On day +4, most TCRbeta + cells divide at least once more while turning back to slow cycle conditions. Most of the TCRbeta - cells maintain a slow cycle throughout the first 3 d of culture with no more than one division per day and a reduction in the proportion of cycling cells to 50% until day +4.

The results of BrdU incorporation suggested that the phase of rapid divisions of TCRbeta + cells only begins on day +1 in FTOC (Fig. 6). In contrast, PI staining showed >60% of TCRbeta + cells to be in S/G2 already in dg 14.5 ex vivo thymic lobes (Fig. 5). The incomplete BrdU incorporation into TCRbeta + cells on day +1 of dg 14.5 FTOC could indicate an intermittant slowdown of proliferation due to the transfer of the thymic lobes to in vitro conditions. Alternatively, it was possible that TCRbeta + cells remain in a prolonged S/G2 phase through dg 14.5 and most of dg 15.5 before actually undergoing their first division. In the latter case, only unlabeled and partially BrdU labeled TCRbeta + cells should be seen in dg 13.5 FTOC incubated with BrdU for 18 h before analysis on day 13.5+1. As shown in Fig. 7 A, the BrdU incorporation patterns after 1 d of culture of dg 13.5 FTOC were similar to those of dg 14.5 FTOC, with an even higher proportion of TCRbeta + cells labeled to saturation. These data suggest that dg 13.5 TCRbeta + thymocytes have at least a similar capacity for proliferation in vitro as have dg 14.5 TCRbeta + cells. Moreover, we reasoned that if the onset proliferation of TCRbeta + cells in vivo was delayed until late dg 15.5, only the cells in S/G2, and not the cells in G1 phase of cell cycle on that day, should have incorporated BrdU injected into the pregnant mothers 18 h earlier. As shown in Fig. 7 B, labeling in vivo was not as efficient as in vitro, and did not permit a quantitative determination of unlabeled, as compared to partially and completely labeled, cells. Nevertheless, the data show that both G1 and S/G2 phase TCRbeta + cells on dg 15.5 incorporated BrdU under these conditions in vivo. These results support our conclusions from CLSM analyses and suggest that TCRbeta + thymocytes start cell divisions as early as dg 14.5 in vivo, and that transfer to in vitro conditions causes a lag in the onset of proliferation. The data in Fig. 7 B further reveal that dg 15.5 and 17.5 TCRbeta - cells in both G1 and S/G2 phases of cell cycle incorporated BrdU injected 18 h earlier into the pregnant mothers. These data support the in vitro results in that they are consistent with a division rate of once per day for the major proportion of TCRbeta - cells. Finally, and also in support of the in vitro results, dg 17.5 TCRbeta -, but not TCRbeta +, cells contain a proportion of hypodiploid cells suggesting ongoing cell death by apoptosis in this population.

The experiment in Fig. 7 C demonstrates that PCR analysis yielded fragments corresponding to TCRbeta VDJ rearrangements in dg 14.5 thymocytes, together with stronger signals for DJ rearrangements and a strong germ line fragment. In contrast to adult thymus, VDJ rearrangements at dg 14.5 display only some of the six Jbeta 2 segments, presumably reflecting the paucity of rearranged TCR Vbeta genes at that time. These data support our serological detection of TCRbeta + cells in dg 14.5 thymocytes.


Discussion

During thymic development, a large number of DP thymocytes arise from a small number of DN cells. While this process most likely involves cell proliferation, the extent of cell division cannot be judged from the pool sizes of DN and DP cells, as these are strongly influenced by parameters unrelated to cell division, such as entry rates, exit rates, and mean survival times (46). The present paper describes an attempt to obtain numerical information on cell divisions that occur during the generation of DP cells, using a combination of morphodynamic studies in situ with cell cycle analyses. To focus on cells that have entered the thymus during a defined temporal window in fetal life, most analyses have been done in thymic organ cultures. While it is clear that the increment in thymic cellularity during fetal life in vivo exceeds that in vitro, a proportion of the additional increment in vivo is obviously due to continuous influx of new cells into the thymus. Another proportion of the difference may be due to suboptimal conditions in vitro, so that numbers and rates of cell divisions determined in these studies obviously represent lower estimates. However, our comparisons of cell accumulation in vitro and in vivo localize the major deficit of FTOC to the first day of culture so that our data provide a reasonable basis for estimates of fetal thymocyte proliferation in vivo.

Differentiation of DN to DP cells is dependent on the expression of the TCRbeta chain, most likely as part of the pre-TCR. Fetal thymocytes expressing TCRbeta polypeptides have first been reported on dg 15.5 (41, 42). We were surprised to see TCRbeta + cells as early as dg 13.5 by CLSM and attribute the increased sensitivity to the capacity to search for individual fluorescent cells by scanning through the entire thymic organ. Since thymic repopulation begins around dg 11 to 12, it appears that TCRbeta rearrangement may either be complete within 2 d of thymic arrival, or that some cells may enter the thymus with previous TCRbeta VDJ rearrangements. The latter possibility is not excluded by our data; although we never saw CD44+TCRbeta + thymocytes on dg 13.5 or 14.5, biosynthesis of the TCRbeta chain may be delayed after rearrangement. However, the developmental stage of TCRbeta gene rearrangement previously determined in adult mice (11) seems to hold for fetal development as well (39, 40).

What are the proportions of TCRbeta + cells arising by de novo induction and by subsequent proliferation? During dg 13.5 and 14.5, most TCRbeta + thymocytes appear as individually dispersed cells in CLSM. On dg 15.5, a greater number of TCRbeta + cells with many doublets are seen, followed by a further increase in density on dg 16.5. Thus, according to CLSM, little cell division occurs for at least one day after thymocytes first express a TCRbeta polypeptide. We therefore think that the ~100 TCRbeta + cells in dg 14.5 fetal thymic lobes almost exclusively arise by independent induction events. In situ analyses of the TCRbeta + population in FTOC shows maintenance of the individually dispersed appearance of TCRbeta + cells until day +1, and BrdU incorporation suggests that about one third of these cells have arisen by cell division. Together, these results suggest that a minimum of 300 of the 400 TCRbeta + cells/thymic lobe after one day of FTOC arose by independent induction events. Our data from CLSM and from BrdU incorporation in vivo suggest that at least half of the 1,200 TCRbeta + cells/thymic lobe on dg 15.5 in vivo may be the products of cell division, suggesting a maximum of 900 independently induced TCRbeta + cells until that time. Together, our data suggest about 600, certainly less than the 1,000 independently induced TCRbeta + cells/thymic lobe until dg 15.5. Thereafter, as shown by BrdU incorporation, TCRbeta + cells accumulate predominantly by cell division. Productive TCRbeta locus rearrangement thus seems to take place in <10% of the cells that have entered the thymus until dg 14.5, i.e., in a rather small proportion of thymocytes. Even after subtraction of ~10% gamma delta thymocytes present at that time, this is significantly less than statistically expected if all remaining thymocytes were induced to rearrange their TCRbeta genes. Induction of TCRbeta locus rearrangement may thus be a relatively inefficient process in early fetal thymocytes.

From day +1 of  FTOC onwards, TCRbeta + cells undergo a series of rapid cell divisions resulting in a nearly 200-fold expansion of this population within the following 3 d. This expansion phase is indeed burst-like; it acquires maximum speed (>=3 divisions/d) on days +2 and +3, and returns to a slow rate of about one-two divisions during the fourth day. Estimates of cell divisions are derived from three mutually supportive sources of information: cell counts by FCM, BrdU incorporation, and morphodynamic studies on the expansion of TCRVbeta clones in situ. The rates of cell division estimated from the latter source are slightly lower, possibly due to some degree of underestimation of clone sizes in CLSM. Alternatively, clones expressing different TCRVbeta products may expand with somewhat different kinetics. The total accumulation of TCRbeta + thymocytes in 4 d of FTOC is ~1/6-1/7 of that determined until the corresponding dg 18.5 in vivo. Half of this difference is due to a slowdown in proliferation during the first day of FTOC, leading to threefold less TCRbeta + cells on day +1 of culture than on dg 15.5 in vivo. Most of the other half can perhaps be accounted for by lack of influx of new precursor cells into the thymus. Thus, we estimate an about 500-fold expansion of TCRbeta clones during the burst in vivo, corresponding to about nine cell divisions within a time frame of ~4 d.

The biological purpose of pre-TCR dependent proliferation appears to be the selection for further maturation of thymocytes with a functional rearrangement of the TCRbeta locus, thus avoiding the further differentiation of useless cells that have failed to productively rearrange a TCRbeta gene. Our analyses of TCRbeta - cells in FTOC reveals an initial proportion of ~30% in S/G2 which declines to <10% over the 4 d of culture. Moreover, an initial proportion of 3/4, declining to 1/2, becomes partially labeled with BrdU during an 18 h pulse. Both sets of data are roughly in agreement and suggest that the majority of TCRbeta - cells are indeed dividing between dg 14.5 and 18.5, but they do so at a slower rate than that of the TCRbeta + cells. With the exception of dg 14.5 to 15.5, the increment of TCRbeta - cells/day is < twofold in vitro and in vivo. This results in an ~8-14-fold increment in TCRbeta - cells until day +4 in FTOC or dg 18.5 in vivo, consistant with one division/day of most, but not all cells. We observed small but significant proportions of apoptotic TCRbeta - cells in vivo and in vitro, indicating that cells that have failed to generate a productive TCRbeta rearrangement eventually die by programmed cell death. The increase in the proportion of nondividing TCRbeta - cells over the 4 d is consistent with the notion that shutdown of proliferation preceeds cell death. Taken together, it appears that the major mechanism of pre-TCR-dependent positive selection of fetal thymocytes is a burst of up to nine rapid cell divisions that begins within less than two day after TCRbeta expression and lasts for about 4 d. In contrast, lack of selection of TCRbeta - cells is reflected in a limited maintenance of slow proliferation, then shutdown of proliferation, and eventual cell death by apoptosis.

What is the cell cycle status of TCRbeta + cells on dg 13.5 and 14.5, i.e., before we can see doublet formation in CLSM? As judged by PI staining >60% of TCRbeta + cells have entered S/G2 phase on dg 14.5, and TCRbeta + cells in G1 of cell cycle on dg 15.5 have incorporated BrdU during an 18 h pulse. These in vivo results suggest that TCRbeta + cells may begin to divide as early as dg 14.5. This conclusion is further supported by our finding that many TCRbeta + cells incorporate saturating amounts of BrdU on day +1 of dg 13.5 FTOC. Nevertheless, our CLSM results suggest a delay period between TCRbeta chain expression and the start of cell proliferation. We could not exactly define this interval but feel that for most thymocytes the burst of rapid divisions does not start until about one day following expression of the TCRbeta chain, and that most of this delay may be spent in the G2 phase of cell cycle. An interesting set of questions arising from these results concerns the potential biological role of a delay before proliferation sets in: Does proliferation perhaps require a second signal, for example through cytokine-cytokine receptor interaction (47). Is the delay phase important for DNA repair subsequent to TCRbeta rearrangement, and how is this delay phase regulated? Recent experiments suggest that p53 may perhaps have an important role in regulating pre-TCR dependent differentiation events (50).

How do these results on fetal thymocytes compare to previous studies on proliferation of adult thymocyte subsets? According to Shortman et al. (35) the maturation of DN to DP thymocytes is accompanied by two phases of proliferation, separated by the CD25+CD44- DN stage in which the cells are resting. The latter subset, at which TCRbeta rearrangement takes place, was found by Penit et al. (36) and by Hoffman et al. (37) to be split into dividing and nondividing cells. Although not directly shown, the dividing and nondividing subsets were interpreted as TCRbeta + and TCRbeta - cells, respectively, and it was speculated that the latter cells would die due to lack of positive selection. A 200-300 fold expansion takes place during the subsequent proliferation phase which begins at the CD25-CD44- DN stage, ends with small resting DP cells, and lasts 6 d (35, 36). As far as they can be compared, our results are roughly in agreement with these data. For example, the numbers of cell divisions and the expansion factors do not appear to drastically differ between fetal and adult thymocytes. Moreover, the dividing TCRbeta - subset in fetal thymus could correspond to the first proliferative phase involving the immature CD44+CD25- population whereas the resting TCRbeta - subset in fetal thymocytes could potentially correspond to the nondividing subset among the CD25+CD44- adult DN cells. The results described in this paper have augmented previous knowledge primarily by firmly establishing the causal connection between the second phase of thymocyte proliferation and the expression of the TCRbeta chain, in addition to providing numerical information on fetal thymocyte proliferation.


Footnotes

Address correspondence to Klaus Eichmann, Max-Planck-Institut für Immunobiologie, Stübewe, 51, D-79108 Freiburg, Germany.

Received for publication 8 August 1996

   1Abbreviations used in this paper: BrdU, 5-bromo-2'-deoxyuridine; CLSM, confocal laser scanning microscopy; dg, day of gestation; DN, double negative; DP, double positive; FCM, flow cytometry; FTOC, fetal thymic organ culture; IC, intracellular; PI, propidium iodide; SP, single positive.

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Copyright © 1996 by The Rockefeller University Press.