ARTICLE |
CORRESPONDENCE Toshinori Nakayama: tnakayama{at}faculty.chiba-u.jp
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CD4 T celldependent immune responses are controlled by the balance of antigen-specific Th1 and Th2 cells (13). IL-12induced activation of STAT4 is crucial for Th1 cell differentiation, whereas IL-4induced STAT6 activation is crucial for Th2 cell differentiation (48). In addition to cytokine-induced signals, the activation of the TCR-mediated signaling is indispensable for both Th1 and Th2 cell differentiation. In particular, Th2 cell differentiation is largely dependent on the activation of p56lck, calcineurin, and the Ras-ERK MAPK signaling cascade (911). A negative regulator of the above signaling pathways, SHP-1, also controls the efficiency of Th2 cell differentiation and Th2 celldependent immune responses (12). Master transcription factors for Th1 and Th2 cell differentiation have been revealed, i.e., GATA3 for Th2 cells and T-bet for Th1 cells (1317).
In Drosophila, Mad-Medea and Schnurri (Shn), a large zinc finger protein, are reported to interact with each other and act as nuclear targets in the Drosophila decapentaplegic signaling pathway (1820). In vertebrates, the Drosophila decapentaplegic signaling pathway may equate to the bone morphogenetic protein/TGF-ß/activin signaling pathways that play diverse roles in developmental processes (21). Vertebrates have at least three orthologues of Shn: Shn-1 (also known as HIV-EP1, MBP-1, PRDII-BF1, and A-CRYBP1), Shn-2 (also known as HIV-EP2, MBP-2, AGIE-BP1, and MIBP1), and Shn-3 (also known as HIV-EP3, KRC, and ZAS3). The mRNA expression of Shn-2 was detected mostly in the brain, heart, and spleen (2224). Recently, the requirement of Shn-2 in positive selection of thymocytes was reported (25) and Shn-3deficient CD4+ CD8+ thymocytes were shown to exhibit a defect in cell survival (26). However, the precise physiological roles of these Shn family members remain largely unknown.
NF-B is a critical transcription factor that regulates Th2 cell differentiation and Th2 celldependent airway inflammation (2729). NF-
Bdeficient (p50 subunitdeficient) mice were unable to mount OVA-induced airway inflammation (28). The lack of inflammation was not due to defects in T cell priming, T cell proliferation, or expression of important cell adhesion molecules, but rather to the impaired induction of GATA3 (30). Recently, one of the Shn family members, Shn-3 (KRC), was reported to associate with TNF receptorassociated factor (TRAF)2 to repress nuclear translocation of NF-
B (31). In addition, Shn-3 (ZAS3) was shown to bind to the NF-
B motif directly, and the competition with NF-
B binding resulted in the repression of transactivation of the NF-
B target gene (32, 33). A direct repressive activity of Shn-3 was also demonstrated (33). These emerging findings suggest that the Shn protein is involved in NF-
B activation and/or NF-
Bmediated gene transactivation.
Here, we investigate the role of Shn-2 in Th1/Th2 cell differentiation by using Shn-2deficient (Shn-2/) mice. Our results suggest that Shn-2 plays crucial roles in the control of Th2 cell differentiation by regulating NF-B activation and GATA3 expression.
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Results |
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Enhanced Th2 cell differentiation in Shn-2/ x DO11.10 transgenic (Tg) naive CD4 T cells
Next, we assessed the capability of Shn-2/ CD4 T cells to differentiate into Th1/Th2 cells. Naive splenic CD4 T cells (CD4+ CD44low) were purified by cell sorting (purity >98%) and then subjected to in vitro Th1/Th2 cell differentiation culture and analyzed by intracellular cytokine staining and ELISA. Naive CD4 T cells from Shn-2/ x OVAspecific TCR-ß Tg (DO11.10 Tg) mice were stimulated with 0.1 µM of antigenic OVA peptide and irradiated BALB/c splenocytes in the presence of appropriate cytokines and anti-cytokine mAbs under three different conditions: Th2-skewed (IL-4 with antiIL-12 mAb and antiIFN-
mAb), Th1-skewed (IL-12 with antiIL-4 mAb), and nonskewed (IL-2 with antiIL-4 mAb, antiIL-12 mAb, and antiIFN-
mAb) conditions. As shown in Fig. 1 A, Th2 cell differentiation was enhanced substantially in Shn-2/ T cells (17.3 vs. 37.1%), whereas Th1 cell differentiation was unaffected (83.0 vs. 80.5%). Under nonskewed conditions, the baseline levels of IL-4producing and IFN-
producing cells were increased in Shn-2/ T cell cultures (Fig. 1 A, left). Portions of the same cell cultures that were used for intracellular cytokine staining were restimulated, and cytokine production was measured by ELISA. Basically, a similar pattern among cultures was observed (Fig. 1 B). The production of IL-4 and IL-5 was increased about twofold in Shn-2/ Th2 cells, whereas similar levels of IFN-
production in Th1 cells were detected. The levels of IL-4 and IFN-
production in nonskewed cultures were very low, but they were slightly increased in the Shn-2/ cultures.
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Enhanced Th2 cell differentiation in Shn-2/ splenic CD4 T cells after anti-TCR mAb stimulation
To further assess the efficiency of Th2 cell differentiation in the splenic Shn-2/ T cells, purified splenic naive CD4 T cells (purity >98%) were stimulated with immobilized antiTCR-ß mAb in the presence of graded doses of exogenous IL-4. No APCs were added. 30 U/ml of exogenous IL-2 was added to the differentiation cultures. As shown in Fig. 2 A, IL-4producing Th2 cells were generated in an exogenous IL-4 dose-dependent manner in the Shn-2+/+ wild-type T cell cultures, and as expected, the generation of Th2 cells in Shn-2/ mice was substantially enhanced in the cultures at all doses of exogenous IL-4 (Fig. 2 A). These results suggest that the ability to differentiate into Th2 cells was enhanced in Shn-2/ splenic CD4 T cells. The number of Th2 cells generated in Shn-2/ x STAT6/ T cell cultures was insignificant, suggesting that the enhanced Th2 cell differentiation in Shn-2/ T cell cultures was STAT6 dependent (not depicted).
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Anti-TCRinduced cell division of Shn-2/ CD4 T cells
Because some cycles of cell divisions are reported to be required for the generation of IL-4producing Th2 cells (34), we examined the anti-TCRinduced cell division of Shn-2/ CD4 T cells. Carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled naive CD4 T cells were stimulated with antiTCR-ß mAb in the presence of IL-2 and IL-4 (Th2 cellskewed condition), and 24, 48, 72, and 96 h later, IL-4 production in the developing Th2 cells was assessed (Fig. 2 C). 24 h after the stimulation, most cells had not undergone cell division (cell division no. 0), and IL-4producing Th2 cells were not detected. At 48 h, cells had undergone up to three cell divisions, and there was a slight increase in the numbers of cells with three cell divisions detected in Shn-2/ CD4 T cells. The percentages of IL-4producing T cells generated were not apparently different between Shn-2/ and Shn-2+/+ CD4 T cell cultures at this time point. After 72 and 96 h in culture, the rate of cell division appeared to be slightly increased in Shn-2/ CD4 T cells, and the generation of IL-4producing T cells was higher at all numbers of cell division. These results suggest that although Shn-2/ CD4 T cells show a slightly increased rate in cell division, the enhanced Th2 cell generation in Shn-2/ CD4 T cells could not be simply explained by the difference in the number of cell divisions that the developing Th2 cells had undergone.
We reported previously that CD28 costimulation induced increased Th2 cell differentiation (35) and induced NF-B activation and enhanced GATA3 expression in developing Th2 cells to facilitate chromatin remodeling of the IL-5 gene loci (36). Shn-2/ T cells were stimulated with anti-TCR plus anti-CD28 mAb under Th2 cellskewed conditions (Fig. S4, available at http://www.jem.org/cgi/content/full/jem.20040733/DC1). Shn-2/ T cells showed increased levels of Th2 cell differentiation without anti-CD28 mAb as compared with wild-type (46.2 vs. 32.4%). No obvious additional enhancement of Th2 cell differentiation was detected in the presence of CD28 costimulation under the conditions where the CD28-mediated enhancing effect was observed in Shn-2+/+ T cell cultures.
No functional defect was observed in DCs from Shn-2/ mice
The results thus far suggested that the observed enhancement of Th2 cell differentiation is due to an alteration in T cells because we used purified T cells (Figs. 1 and 2) and APCs from normal mice (Fig. 1). However, Shn-2 mRNA is expressed in both CD4 T cells and APCs (not depicted), and thus, we wished to evaluate the effect of Shn-2 deficiency on APC function using bone marrowderived DCs (BMDCs). The expression levels of CD11c, I-Ad, H-2Kd, CD40, B7-1, B7-2, and Fas were comparable between Shn-2+/+ and Shn-2/ BMDCs (not depicted). OVA-pulsed BMDCs from Shn-2+/+ or Shn-2/ mice were cocultured with DO11.10 Tg CD4 T cells, and proliferative responses of T cells were assessed by [3H]thymidine uptake. Under these conditions, the APC function of both immature and mature BMDCs was found to be comparable between Shn-2/ or Shn-2+/+ mice (Fig. 3 A). We then examined the ability of OVA-pulsed BMDCs as APCs to induce Th1/Th2 cell differentiation. Stimulation with either immature or mature BMDCs resulted in similar levels of Th1/Th2 cell differentiation in the cultures with Shn-2+/+ and Shn-2/ BMDCs (Fig. 3 B). To further evaluate the APC function of Shn-2/ mice, spleen cells were irradiated and tested for their ability to induce Th1/Th2 cells. The numbers of Th2 cells generated were found to be comparable between cultures with Shn-2/ and Shn-2+/+ APCs (Fig. 3 C). Taken together, the enhanced Th2 cell generation in Shn-2/ T cell cultures appears to be due to an alteration in CD4 T cells themselves and not to any change in APC function.
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We also examined the activation of AP-1, CREB, and IL-4 NFAT in Shn-2/ CD4 T cells by EMSA (Fig. S5, available at http://www.jem.org/cgi/content/full/jem.20040733/DC1). The DNA binding ability of AP-1 was slightly, but reproducibly, enhanced in Shn-2/ T cells, whereas the DNA binding activity of CREB and IL-4 NFAT appeared to be similar in Shn-2+/+ and Shn-2/ T cells.
Enhanced expression of GATA3 in anti-TCRactivated Shn-2/ CD4 T cells
Because the levels of GATA3 expression are critical for Th2 cell differentiation (14) and because GATA3 expression is regulated by NF-B (30), we next assessed the protein expression of GATA3 in cultured Shn-2/ T cells under Th2 cellskewed condition. Splenic CD4 T cells from Shn-2/ mice were stimulated with antiTCR-ß mAb and IL-4, and 16 h later, the expression of GATA3 was assessed by immunoblotting with specific antibodies. The levels of GATA3 protein were found to be reproducibly increased in Shn-2/ T cells (Fig. 5 A). No detectable GATA3 protein was observed in freshly prepared Shn-2/ CD4 T cells (not depicted). Concurrently, the mRNA levels were assessed by semiquantitative RT-PCR analysis, and increased GATA3 mRNA levels were detected (Fig. 5 B). These results suggest that enhanced induction of GATA3 takes place in early developing Shn-2/ Th2 cells as compared with that of control Shn-2+/+ Th2 cells.
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Shn-2 inhibits an NF-Bdependent transcriptional activity
The ability of Shn-2 protein to bind to the consensus NF-B binding motif was assessed. 293 T cells were transfected with a pAct-Flag-hShn-2 vector, and 2 d later, nuclear extracts were prepared. Biotinylated NF-
B and control AP-1 oligonucleotides were absorbed on to streptavidin-agarose beads and incubated with the nuclear extracts. The amount of Shn-2 protein in the precipitates of agarose beads was assessed by immunoblotting with anti-Flag mAb. Total nuclear extracts were also run in parallel as loading controls. As can be seen in Fig. 6 A, a substantial binding of FlagShn-2 protein to NF-
B oligo was observed. This result suggests that Shn-2 protein is able to bind to a consensus NF-
B binding motif.
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Consequently, to determine whether Shn-2 protein plays a functional role in NF-Bdependent transcription, NF-
Binduced luciferase assay was performed with 293 T cells transfected with the 5x NF-
B reporter constructs along with graded doses of pAct-Flag-hShn-2 vector after stimulation with PMA plus ionomycin or TNF-
(Fig. 6 C). Some level of luciferase activity was detected without stimulation, and a significant inhibition was observed in the presence of Shn-2. After PMA plus ionomycin stimulation, the luciferase activity was increased and this was efficiently inhibited by Shn-2 (Fig. 6 C, middle). The luciferase activity was increased dramatically by stimulation with TNF-
, and this was moderately inhibited in the presence of Shn-2 protein (Fig. 6 C, right). These results suggest that Shn-2 protein is able to inhibit the NF-
Bdependent transcriptional activity.
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Discussion |
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The DNA binding of NF-B was enhanced in Shn-2deficient CD4 T cells (Fig. 4, A and B). The enhancement was already apparent in nonstimulated Shn-2deficient CD4 T cells. However, we found the NF-
B protein equally expressed in the nucleus and cytoplasm of nonstimulated and stimulated Shn-2deficient CD4 T cells (Fig. 4 C). Thus, the enhanced DNA binding of NF-
B might be regulated by nuclear events such as those involved in the DNA binding process. A previous report suggested that Shn-3 binds to a NF-
B motif and represses transactivation of NF-
Bdependent genes (33). We show here that Shn-2 protein is able to bind to a consensus NF-
B motif (Fig. 6 A) and compete with p50 for DNA binding in a dose-dependent manner (Fig. 6 B). In addition, we detected significant inhibition of the NF-
Bdependent transcriptional activity in 293 T cells. Thus, it is conceivable that Shn-2 is able to bind to NF-
B DNA binding motifs and thus compete with the binding of NF-
B complexes or to repress directly the transactivation of the NF-
Bdependent genes in early developing Th2 cells. This might be a mechanism to account for the elevated NF-
B DNA binding activity in nonstimulated Shn-2deficient CD4 T cells (Fig. 4, A and B).
In addition, we detected a further enhancement of DNA binding of NF-B after TCR stimulation (Fig. 4, A and B). This could explain the enhanced induction of GATA3 in early developing Shn-2/ Th2 cells (Fig. 5, A and B). A similar regulation was demonstrated in an experimental system using p50/ mice (30). In our preliminary results, the generation of IL-4producing Th2 cells in wild-type BALB/c CD4 T cell cultures was increased by the ectopic expression of both p50 and p65 (unpublished data). Thus, it appears probable that the enhanced induction of GATA3 by NF-
B hyperactivation is a mechanism that is responsible for the enhanced Th2 cell differentiation observed in Shn-2deficient T cells.
Another interesting possibility for the molecular targets of Shn-2 is the involvement of TRAF2-mediated NF-B activation. A previous report suggested that there is a physical association of Shn-3 with TRAF2 (31). Our preliminary results suggest that a physical association of Shn-2 with TRAF2 in 293 T cells can be demonstrated (unpublished data). Thus, Shn proteins may bind to TRAF2 and inhibit the TNFR-induced NF-
B activation and nuclear translocation in T cells. However, this appeared to be unlikely because no obvious difference in the protein expression of p50 and p65 was observed in the cytosol or nucleus before or after anti-TCR stimulation between Shn-2+/+ and Shn-2/ T cells (Fig. 4 C). Lieberson et al. (37) have reported that Th2 cell responses are enhanced in dominant-negative TRAF2 Tg CD4 T cells. This result would appear to be contradictory to the above hypothesis. However, the dominant-negative TRAF2 used in the study by Lieberson et al. inhibits JNK activity efficiently, but it does not affect NF-
B activity very significantly (38). Thus, it is still possible that the Shn-2TRAF complex is formed in T cells and that it is involved in the control of Th2 cell differentiation by regulating NF-
B activation. A more precise investigation is needed to clarify this issue.
As for the signaling pathways in which Shn-2 is involved, Shn-2 may regulate outcomes of both the TCR- and TNF-dependent pathways if the major function of Shn-2 is mediated by a mechanism proposed above, namely, competition with NF-B for DNA binding in the nucleus. In this paper, we focused our analysis mostly on T cells where the TNF-dependent signaling pathway is not activated efficiently, and thus, further investigation will be required to address this issue. In addition, we did not observe CD28 costimulation-mediated enhancement of Th2 cell differentiation in Shn-2/ cells (Fig. S4). CD28 costimulation induced NF-
B activation and enhanced GATA3 expression in developing Th2 cells (36). Thus, it is possible that the effect of CD28 costimulation was masked by hyperactivation of NF-
B in Shn-2/ T cells.
It has been established that the expression of GATA3 is crucial for the efficient Th2 cell differentiation (16). GATA3 plays an essential role in chromatin-remodeling processes, such as acetylation of histone H3 and H4 of the Th2 cell cytokine gene loci (36). The activation of STAT6 is most critical for the induction of GATA3 transcription (16, 39). More recently, several regulatory molecules for GATA3 transcription have emerged. One is the NF-B activation discussed above (30). We reported previously that a polycomb group gene product, mel-18, controls Th2 cell differentiation by regulating GATA3 transcription (40). Shn-2 is another example of a protein that regulates GATA3 transcription and expression in developing Th2 cells. Further investigation of the regulation of GATA3 expression is likely to provide a clearer molecular view of the initiation of Th2 cell differentiation.
Recombinant TGF-ß inhibited Th2 cell differentiation of normal and Shn-2deficient T cells very efficiently (unpublished data). In addition, Smad7 expression was not changed in Shn-2deficient T cells (unpublished data). Thus, Shn-2 may not be involved in the TGF-ßmediated signaling pathway in T cells, at least not in the TGF-ßmediated inhibition of Th2 cell differentiation. However, because mammalian Shn has three orthologues (Shn-1, Shn-2, and Shn-3), it is still possible that Shn-1 and/or Shn-3 could substitute for the function of Shn-2 in Shn-2deficient T cells.
As for the effects of Shn-2 deficiency on Th1 cell differentiation, Shn-2 may not play critical roles in Th1 cell differentiation. No effect was observed in typical Th1 cellskewed cultures containing excess amounts of IL-12 (Fig. 1 A). However, we observed some enhancement in Th1 cell differentiation when IL-12 is limiting (Fig. 2 B). Also, IFN-producing cells generated under Th2 cellskewed conditions were increased consistently (Fig. 1 C). Thus, it is still possible that Shn-2 may act as a negative regulator of the generation of IFN-
producing cells.
In summary, the results of this study indicate that Shn-2 plays crucial roles in the control of Th2 cell differentiation by regulating the activation of NF-B and subsequent GATA3 induction in early developing Th2 cells.
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Materials and Methods |
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Immunofluorescent staining and flow cytometry analysis
In general, 106 cells were incubated on ice for 30 min with the appropriate staining reagents according to a standard method (42). The reagents used in this study were as follows: anti-CD4PE (RM4-1PE), anti-CD4FITC (RM4-1FITC), anti-CD44FITC (IM7-FITC), anti-CD44PE (IM7-PE), anti-CD69FITC (H1.2F3), anti-CD62LFITC (MEL-14), anti-CD25FITC (7D4), antiIL-4R antibody, and anti-
C antibody-PE were purchased from BD Biosciences. Antirat Ig-FITC was purchased from CAPPEL. AntiTCR-ßFITC (H57-FITC), anti-CD3FITC (2C11-FITC), and anti-CD8Cy5 were prepared in our laboratory. Flow cytometry analysis was performed on a FACSCalibur (Becton Dickinson), and results were analyzed with CELLQuest software (Becton Dickinson). Intracellular staining of IL-4 and IFN-
was performed as described previously (11). FITC- conjugated antiIFN-
antibody (XMG1.2), APC-conjugated antiIL-5 antibody, and PE-conjugated antiIL-4 antibody (11B11; all from BD Biosciences) were used for detection.
Cell purification
Splenic CD4 T cells were purified by using magnetic beads and an Auto-MACS Sorter (Miltenyi Biotec) yielding a purity of >98%. Where indicated, CD4 T cells with naive phenotype (CD44low) were isolated from spleens on a FACSVantage cell sorter (Becton Dickinson) yielding a purity of >98% as described previously (11).
Bone marrow DC cultures
A modified method of Inaba et al. (43) and Chen-Woan et al. (44) was used for bone marrow DC cultures. In brief, bone marrow cells depleted of FcR+ cells (0.51.0 x 106 cells) were cultured in 1 ml medium in the presence of 10 ng/ml GM-CSF. The culture medium was changed every other day by gently swirling the plates, aspirating 75% of the medium, and adding back fresh medium with cytokines. On day 6, the cells were harvested and then CD11c+ cells (immature DCs) were purified using anti-CD11c mAb-conjugated micro beads and a MACS LS column (Miltenyi Biotec). The purified immature CD11c+ DCs were cultured for an additional 2 d with 10 ng/ml GM-CSF and 1 µg/ml LPS to allow further maturation and were used as mature DCs.
Proliferation assay
2 x 105 splenic CD4 T cells were stimulated in 200-µl cultures for 40 h with immobilized antiTCR-ß mAb (H57-597) and anti-CD3 mAb (2C11). Immature or mature DCs were pulsed with 1 µM OVA peptide for 2 h at 37°C and then cocultured with CD4 T cells from DO11.10 Tg mice for 72 h. [3H]thymidine (37 kBq/well) was added to the stimulation culture for the last 16 h, and the incorporated radioactivity was measured by using a ß plate (40).
ELISA for the measurement of cytokine concentration
The production of IL-2, IL-4, IL-5, IL-13, and IFN- was measured by ELISA as described previously (40).
In vitro Th1/Th2 cell differentiation cultures.
Naive splenic CD4 T cells were stimulated with 3 µg/ml of immobilized antiTCR-ß mAb (H57-597) in the presence of 30 U/ml IL-2 and 1100 U/ml IL-4 as described previously (10). Where indicated, naive splenic CD4 T cells were labeled with CFSE (Molecular Probes) using the same procedure as described previously (40). For Th1 cell differentiation, naive splenic CD4 T cells were stimulated with 3 µg/ml of immobilized antiTCR-ß mAb (H57-597) in the presence of 30 U/ml IL-2, IL-12, and antiIL-4 mAb. 1.5 x 104 sorted DO11.10 Tg CD44low CD4 T cells were stimulated with antigenic OVA peptide (Loh15, OVA; 323339) and 105 irradiated (3,000 rad) normal BALB/c splenocytes. For stimulation with purified DCs, 2 x 105 sorted DO11.10 Tg CD44low CD4 T cells were cocultured with 2 x 104 OVA-pulsed immature or mature DCs. Appropriate cytokines and anti-cytokine antibodies were added in Th1/Th2 cell differentiation cultures as described previously (11). In typical DO11.10 Tg T cell cultures, Th2 cellskewed (IL-4 with antiIL-12 mAb and antiIFN- mAb), Th1 cellskewed (IL-12 with antiIL-4 mAb), and nonskewed (IL-2 with antiIL-4 mAb, antiIL-12 mAb, and antiIFN-
mAb) conditions were used.
Immunoblotting
The Immunoblotting for the detection of GATA3, tubulin-, NFAT1, and NFAT2 was performed as described previously (45). For NF-
B detection, anti-p65 (F-6), anti-p50 (E-10), and antihistone H1 (AE-4, all from Santa Cruz Biotechnology, Inc.) antibodies were used.
EMSA
EMSAs were performed using Gel Shift Assay Systems (Promega) according to the manufacturer's instructions. In brief, nuclear extracts were incubated at 4°C with 32P-labeled double-stranded oligo (NF-B oligo; AGTTGAGGGGACTTTCCCAGGC, AP-1 oligo; CGCTTGATGAGTCAGCCGGAA, CREB oligo; AGAGATTGCCTGACGTCAGAGAGCTAG, IL-4 NFAT; oligo; and GTAATAAAATTTTCCAATGTAAA) in DNA binding buffer (Promega). For supershift assays, nuclear extracts were incubated with anti-p50 (C-19; Santa Cruz Biotechnology, Inc.) or anti-p65 (F-6; Santa Cruz Biotechnology, Inc.) antibody at 4°C for 60 min and then incubated with 32P-labeled NF-
B double-stranded oligo at 4°C. Electrophoresis was performed on a 4% native polyacrylamide gel (0.5x TBE; acrylamide/bisacrylamide, 29:1), and the radioactivity was visualized by autoradiography.
Pull-down assay
A detailed protocol was described elsewhere (46). In brief, 293 T cells were transfected with 1.0 µg pAct or pAct-Flag-hShn-2, and 2 d later, the transfected cells were lysed for immunoblotting with anti-Flag mAb (M2; Sigma-Aldrich). Cell lysates were incubated with biotinylated oligonucleotides of NF-B (AGTTGAGGGGACTTTCCCAGGC) and AP-1 (CGCTTGATGAGTCAGCCGGAA). The bound protein were eluted and separated on an SDS polyacrylamide gel and then subjected to immunoblot analysis using specific antibodies (anti-Flag Tag; M2; Sigma-Aldrich). For the competition assay shown in Fig. 6 B, 293 T cells were transfected with pAct-Flag-hShn-2 or pCMX-p50, and their cell extracts were mixed in certain ratios and then the mixed extracts were incubated with NF-
B oligonucleotides as described above. pCMX-p50 was provided by J. Inoue (University of Tokyo, Tokyo, Japan). Anti-Flag Tag (M2; Sigma-Aldrich) mAb and anti-p50 (E-10; Santa Cruz Biotechnology, Inc.) mAb were used for detection.
Transfection and luciferase assays
293 T cells were transfected with 5x NF-B luciferase reporter plasmid together with pAct vector or pAct-Flag-hShn-2 vector and pRL-TK as normalization control. Transfected cells were stimulated with or without 50 ng/ml PMA and 500 nM ionomycin or 10 ng/ml TNF-
for 12 h before luciferase assays (Promega).
PCR analysis
Total RNA was isolated from cultured cells using the TRIzol reagent. Reverse transcription was performed with Superscript II RT (Invitrogen). Threefold serial dilutions of template cDNA were performed. The PCR reaction was performed as described previously (46). The primers used are as follows: ß actin forward, GAGAGGGAAATCGTGCGTGA-3'; ß actin reverse, 5'-ACATCTGCTGGAAGGTGGAC; GATA3 forward, gAAggCATCCAgACCCgAAAC-3'; GATA3 reverse, 5'-ACCCATggCggTgACCATgC; Shn-2 forward, ggAAAgAgggAAAggAgAgATTCACggAgAT-3'; and Shn-2 reverse, 5'-ATCTgAgTgTCATCACAAgAgTCACTgggT.
For quantitative PCR assay, first-strand cDNA was synthesized using random primers and Superscript II RT (Invitrogen). Samples were then subjected to real-time PCR analysis on an ABI PRISM 7000 Sequence Detection System (Applied Biosystems) under standard conditions. The primers and TaqMan probes for the detection of p50, p65, T-bet, and 18S were purchased from Applied Biosystems. p50, p65, and T-bet expression were normalized using the 18S signal.
Online supplemental material
Fig. S1 shows the phenotypic feature of splenic CD4 T cells from Shn-2/ mice. (A) The yields of thymocytes and splenocytes are shown in boxed numbers. (B) Each histogram depicts the expression of the indicated marker antigens on electronically gated splenic CD4 T cells. Background staining is shown as hatched areas. (C) The histogram shows the percentages of indicated Vß cells among splenic CD4 T cells. Fig. S2 shows the proliferative responses and Erk phosphorylation status after TCR stimulation. Arbitrary densitometric units are shown under each band in B. Fig. S3 shows the expression of Shn-2 mRNA in developing Th1 and Th2 cells. The mRNA levels were determined by RT-PCR with threefold serial dilutions of template cDNA. Fig. S4 shows the effects of CD28 stimulation on Th2 cell differentiation. Gel shift assay for AP-1, CREB, or IL-4 NFAT are shown in Fig. S5. Figs. S1S5 are available at http://www.jem.org/cgi/content/full/jem.20040733/DC1.
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Acknowledgments |
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This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology (Japan; Grants-in-Aid for Scientific Research; Priority Areas Research nos. 13218016 and 16043211; Scientific Research B no. 14370107; Scientific Research C nos. 16616003 and 15790248; and Special Coordination Funds for Promoting Science and Technology), the Ministry of Health, Labor and Welfare (Japan), the Program for Promotion of Fundamental Studies in Health Science of the Organization for Pharmaceutical Safety and Research (Japan), The Japan Health Science Foundation, Uehara Memorial Foundation, Kanae Foundation, and the Mochida Memorial Foundation.
The authors have no conflicting financial interests.
Submitted: 14 April 2004
Accepted: 11 November 2004
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References |
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