Estrogen Protects Lenses against Cataract Induced by Transforming Growth Factor-beta (TGFbeta )

By Angela M. Hales, Coral G. Chamberlain, Christopher R. Murphy, and John W. McAvoy

From the Department of Anatomy and Histology, and Institute for Biomedical Research (F13), The University of Sydney, Sydney, New South Wales, Australia 2006

Summary
Materials and Methods
Results
Discussion
Footnotes
References


Summary

Cataract, already a major cause of visual impairment and blindness, is likely to become an increasing problem as the world population ages. In a previous study, we showed that transforming growth factor-beta (TGFbeta ) induces rat lenses in culture to develop opacities and other changes that have many features of human subcapsular cataracts. Here we show that estrogen protects against cataract. Lenses from female rats are more resistant to TGFbeta -induced cataract than those from males. Furthermore, lenses from ovariectomized females show increased sensitivity to the damaging effects of TGFbeta and estrogen replacement in vivo, or exposure to estrogen in vitro, restores resistance. Sex-dependent and estrogen-related differences in susceptibility to cataract formation, consistent with a protective role for estrogen, have been noted in some epidemiological studies. The present study in the rat indicates that estrogen provides protection against cataract by countering the damaging effects of TGFbeta . It also adds to an increasing body of evidence that hormone replacement therapy protects postmenopausal women against various diseases.


The mammalian lens has a highly organized cellular architecture. A monolayer of cuboidal epithelial cells covers the anterior surface of the elongated fiber cells that constitute the bulk of the lens. These cellular elements are enclosed within a thickened basement membrane, the lens capsule. Normally, the lens transmits light and focuses it onto the retina. Cataract, or clouding of the ocular lens, is associated with disruption of normal cellular architecture. This condition is a major cause of visual impairment and is likely to become an even greater public health problem as the world population ages. Predisposing factors include aging, diabetes, UV/sunlight, ocular surgery, and malnutrition (1).

A role for female hormones in protecting against cataract has been suggested by recent epidemiological studies. The prevalence of cataract increases in postmenopausal women (2, 3). Moreover, postmenopausal women on hormone replacement therapy or younger women taking oral contraceptives display a decreased prevalence and severity of cataract (4).

We have developed a lens culture system for investigating cataractogenesis and factors that influence it. Using this system, we have shown that transforming growth factor-beta (TGFbeta ) induces responses in lens cells that mimic events in cataractogenesis. Rat lens epithelial explants (8, 9) and lenses (10) cultured with TGFbeta undergo molecular and morphological changes that are typically associated with anterior subcapsular cataract and with the subcapsular opacification that often arises from cells left behind after cataract surgery (aftercataract). Some of these changes are also known to be associated with posterior subcapsular cataract. It is significant that TGFbeta induces distinct anterior subcapsular opacities in cultured rat lenses (10). Histologically, these correspond with subcapsular plaques of aberrant cells which are virtually indistinguishable from early stage anterior subcapsular cataracts in humans (see reference 11). Two molecular markers for subcapsular cataract, alpha -smooth muscle actin and type I collagen, are also present. The finding that TGFbeta is cataractogenic is consistent with many studies which now indicate that TGFbeta is involved in the aetiology of diseases in other organs (for example see references 12 and 13). These diseases are generally associated with fibrotic changes.

In the present study, using our lens culture system, we have shown that lenses from female rats are less susceptible to the cataractogenic effects of TGFbeta than those from males. In addition, using whole lenses from ovariectomized rats, we have established that resistance to TGFbeta -induced cataract is conferred by estrogen.


Materials and Methods

17-beta -estradiol (1,3,5[10]-Estratriene-3, 17beta -diol) and progesterone (4-Pregnene-3, 20-dione) were obtained from Sigma Chemical Co. (St. Louis, MO) and human recombinant TGFbeta 2 from Genzyme Corp. (Cambridge, MA). In some experiments, lenses were derived from normal 6-10-mo-old adult male and female Wistar rats, killed by CO2 asphyxiation before removal of eyes. Alternatively, ovariectomies were performed on 3-mo-old female Wistar rats under anaesthesia as described elsewhere (14). After waiting for 4-5 wk to ensure clearance of residual estrogen and progesterone, ovariectomized rats were given three daily injections of 0.5 µg 17-beta -estradiol or 5 mg progesterone, dissolved in benzyl alcohol/peanut oil (1:3, vol/vol). Control rats received vehicle alone. 1 d later, rats were sacrificed by a lethal dose of nembutal (Boehringer Ingelheim, Sydney, Australia) before removal of eyes.

Lens Cultures. Lenses were carefully dissected free from surrounding ocular tissues in culture medium, as described previously (10), and cultured with TGFbeta 2 at final concentrations of 0.025-4 ng/ml, which was added immediately unless otherwise indicated. Controls received no TGFbeta . Culture medium was renewed every 2 d throughout the culture period, without further addition of TGFbeta . Lenses were cultured for 7 d and the anterior surface was photographed daily to record development of opacities. At the end of the culture period, lenses were fixed in Carnoy's fixative (acetic acid/ethanol, 1:3, vol/vol) and embedded in paraffin.

Generally, serum-free medium 199 containing antibiotics and 0.1% bovine serum albumin, as already described (10), was used as culture medium. When testing the effect of estrogen in vitro, medium 199 was replaced by phenol red-free minimum essential medium (Sigma Chemical Co.).

Opacification Index. TGFbeta -induced lens opacification begins as diffuse clouding on the anterior surface of the lens. As the response progresses, these regions condense to form distinct opacities leaving a reduced area of clouding. At low concentrations of TGFbeta , few distinct opacities are observed at day 7 of culture, and a large proportion of the lens surface remains cloudy. In contrast, most of the initially cloudy areas condense to form numerous distinct opacities at high concentrations (as in Fig. 1 A). On the basis of these observations, a method for measuring the extent of opacification has been developed (15).


Fig. 1. Comparison of response of lenses from male and female rats to TGFbeta . Lenses were cultured with 0.15 ng/ml TGFbeta 2 and photographed after 7 d. Lenses from male rats (A) developed distinct anterior opacities (arrow). Lenses from females remained transparent (B). Some flaring of the light source is evident in the upper righthand quadrant of each lens. Bar, 400 µm.
[View Larger Version of this Image (75K GIF file)]

Micrographs of lenses at day 7 of culture were used to determine lens opacification. Each micrograph was scanned with an x-ray scanner (3CX; XRS Corp., Torrance, CA) using Adobe Photoshop and XRS Omni Media software. A series of measurements was then made using NIH Image v1.52. In some micrographs, flared reflections of the light source made it impossible to assess the extent of opacification in certain regions (for example see Fig. 1 B). Only micrographs in which the assessable area represented >75% of the total area were used. The assessable area (A) and, within this area, the total area of clouding (B), and the total number of distinct opacities (C), were measured. An "opacification index" was then calculated as follows:

Opacification:index=<FR><NU>number:of:distinct:opacities(C)</NU><DE>proportion:of:assessable:area:with:clouding(B/A)</DE></FR>

Histology and Immunohistochemistry. Serial sections of paraffinembedded lenses were processed for routine histology or for immunohistochemical localization of alpha -smooth muscle actin or type I collagen, as already described (10). Representative lenses from each treatment group were examined by routine histology (all TGFbeta 2 concentrations) and immunolocalisation (0.15 ng/ml TGFbeta 2).


Results

Male-Female Difference in Responsiveness of Lenses to TGFbeta .

Lenses from male rats developed distinct anterior opacities when cultured with 0.15 ng/ml TGFbeta 2 (Fig. 1 A). In contrast, lenses from female rats remained transparent under these conditions (Fig. 1 B), as did control lenses from male and female rats cultured without TGFbeta (not shown; see reference 10). However, at a higher concentration of TGFbeta 2 (1 ng/ml), lenses from female rats also developed opacities (Table 1). For both sexes, the response increased significantly with concentration of TGFbeta .

Table 1. TGFbeta -induced Opacification in Lenses from Adult Rats


TGFbeta Opacification index
Male Female

ng/ml
0   0   0
0.025  13 ± 0.3   0
0.15  57 ± 5   0
1  69 ± 9  54 ± 6
4 187 ± 19 118 ± 10*

Lenses from adult male and female rats were cultured for 7 d. TGFbeta 2 was used at the concentrations indicated. The opacification index was calculated as described in Materials and Methods. Values represent the mean ± SEM of determinations for four individual lenses.
*  This value is significantly lower than the corresponding value for lenses from male rats (P <0.05 Student's t test).

Histological examination of lenses cultured with 0.15 ng/ml TGFbeta 2 revealed distinct subcapsular plaques, containing spindle-shaped cells and extracellular matrix (see reference 10) in the lenses from males (not shown). In contrast, the lenses from females and controls retained normal lens architecture, with a monolayer of epithelial cells overlying the fiber mass. Immunolocalization of alpha -smooth muscle actin and type I collagen showed that, for males, strong reactivity for both of these cataract markers was present in plaques induced by culturing with 0.15 ng/ml TGFbeta 2, whereas corresponding lenses from females showed no reactivity for alpha -smooth muscle actin and only very weak reactivity for type I collagen in a few cells in the epithelium (not shown). These markers were not detectable in sections of control lenses from males or females cultured for =<7 d without TGFbeta (not shown).

Effect of Hormone Replacement after Ovariectomy on TGFbeta induced Cataract.

Having established that lenses from female rats showed more resistance to the cataractogenic effects of TGFbeta than those from males, ovariectomized rats were used to assess the contribution of ovarian hormones to this phenomenon. Lenses from ovariectomized rats (without hormone replacement) developed opacities when cultured with 0.15 ng/ml TGFbeta (Table 2; Fig. 2 A), a concentration shown to have negligible effect on lenses from normal female rats (Table 1; Fig. 1 B). Lenses from ovariectomized rats which received estrogen, however, did not develop opacities under these conditions (Table 2; Fig. 2 B), while the response of lenses from rats treated with progesterone was similar to that of lenses from vehicle-treated rats (Table 2; Fig. 2 C).

Table 2. TGFbeta -induced Opacification in Lenses from Ovariectomised Rats on Various Hormone Replacement Regimes


Hormone regime Opacification index

Vehicle alone        240 ± 18
Estrogen          0
Progesterone 210 ± 19*

TGFbeta 2 (0.15 ng/ml) was used and lenses were cultured for 7 d. The opacification index was calculated as described in Materials and Methods. Values represent the mean ± SEM of determinations for six individual lenses.
*  This value is not significantly different from the value for controls (vehicle alone).


Fig. 2. Influence of ovarian hormones on induction of cataract by TGFbeta . Ovariectomized rats received vehicle alone (A), estrogen replacement (B), or progesterone replacement (C). Lenses were cultured with 0.15 ng/ml TGFbeta 2 and photographed after 7 d. Lenses from rats that received vehicle alone or progesterone developed distinct opacities (A and C), whereas lenses from rats that received estrogen remained transparent (B). Bar, 400 µm.
[View Larger Version of this Image (46K GIF file)]

Histologically, the opacities observed in ovariectomized rats that only received vehicle corresponded with subcapsular plaques or clumps of abnormal cells (Fig. 3 A). Reactivity for the cataract markers alpha -smooth muscle actin and type I collagen was observed predominantly within the subcapsular plaques (Fig. 3, C and E). In contrast, lenses from estrogen-treated rats retained normal cellular morphology (Fig. 3 B), and no reactivity for alpha -smooth muscle actin or type I collagen was detected (Fig. 3, D and F). In all these respects, lenses from rats that received progesterone replacement were indistinguishable from lenses from rats that received vehicle alone.


Fig. 3. Histology and immunolocalization of cataract markers. Lenses from ovariectomized rats that received vehicle alone (A, C, and E) or estrogen replacement (B, D, and F) were cultured with 0.15 ng/ml TGFbeta 2 and fixed at the end of a 7 d culture period. Serial sections were stained with haematoxylin and eosin (A and B), or used for localization of alpha -smooth muscle actin (C and D) and type I collagen (E and F). Lenses from rats that received vehicle alone developed large anterior subcapsular plaques (A), which contained spindle-shaped cells (arrow) and many condensed nuclei. In addition, the fiber cells around the plaques appeared swollen and vacuoles were commonly present (asterisk). alpha -Smooth muscle actin (C) and type I collagen (E) were localized within the TGFbeta induced subcapsular plaques and also in some of the cells that remained attached to the capsule (arrowheads). Lenses from rats that received estrogen retained normal lens morphology (B) with a monolayer of epithelial cells (ep) adjacent to the lens capsule (ca) and overlying the fibre cells (fc). These lenses showed no reactivity for either alpha -smooth muscle actin (D) or type I collagen (F). Bar, 40 µm.
[View Larger Version of this Image (100K GIF file)]

A variety of more subtle histological changes were observed in lenses from ovariectomized rats that did not receive estrogen. Swelling of cortical fiber cells with evidence of degeneration, reminiscent of cortical cataract (11), was commonly observed (for example see Fig. 3 A), generally in the region of the lens anterior to the equator. In addition, nucleated cells were observed migrating along the posterior capsule towards the posterior pole (Fig. 4 A); these cells showed reactivity for type I collagen (Fig. 4 C), but not for alpha -smooth muscle actin. None of these changes were observed in lenses from estrogen-treated ovariectomized rats, which remained transparent (Fig. 4, B and D). In lenses from normal male and female rats (not shown), concentrations of TGFbeta >0.15 ng/ml were required to induce changes as pronounced as those in Figs. 3 A and 4 A. Thus, lenses from normal rats of either sex seemed to be more resistant to the effects of TGFbeta than those from ovariectomized rats that did not receive estrogen.


Fig. 4. Posterior migration of cells associated with induction of cataract by TGFbeta . Lenses from ovariectomized rats that received vehicle alone (A and C) or estrogen replacement (B and D) were cultured with 0.15 ng/ml TGFbeta 2 and fixed at the end of the 7 d culture period. Serial sections were stained for routine histology with haematoxylin and eosin (A and B) or used for immunofluorescent localization of type I collagen (C and D). The lens equator is positioned at the top of each micrograph. In lenses from rats that received vehicle alone (A), nucleated cells were observed migrating along the lens capsule toward the posterior pole (A, arrowheads). Strong reactivity for type I collagen was associated with these posteriorly migrating cells (C, arrowheads). In contrast, lenses from rats that received estrogen replacement maintained a normal lens morphology with no abnormal migration of cells below the lens equator (B). No reactivity for type I collagen was observed (D). Bar, 40 µm.
[View Larger Version of this Image (59K GIF file)]

Estrogen administered in vivo as in the above experiments may exert its effects on lens cells directly or indirectly. To determine whether estrogen is capable of influencing lens cells directly, lenses from ovariectomized rats were treated with estrogen in vitro before and during culture with TGFbeta (Table 3). Estrogen-treated lenses showed negligible opacification in response to TGFbeta . While a small region of haziness was observed at the center of some of these lenses within 6 d of adding TGFbeta , no tendency to condense into discrete opacities with time was noted (not shown). Numerous distinct opacities developed in corresponding lenses cultured in parallel without the addition of estrogen, which were thus highly susceptible to the cataractogenic effects of TGFbeta under these conditions (Table 3).

Table 3. Effect of Estrogen Exposure In Vitro on TGFbeta -induced Opacification of Lenses from Ovariectomized Rats


Treatment Opacification index

17-beta -estradiol       0
No estradiol 151 ± 19

Lenses from ovariectomized rats were precultured for 2 d with or without 10-10 M 17-beta -estradiol, as indicated. Medium was then replaced (with or without estradiol, as before) and TGFbeta 2 (0.15 ng/ml) was added immediately. After a further 7 d culture, the opacification index was determined as described in Materials and Methods. Values represent the mean ± SEM of determinations for three individual lenses.


Discussion

The present study grew out of our findings that TGFbeta induces lens epithelial explants and cultured lenses to undergo molecular and morphological changes that are typically associated with human subcapsular cataracts (8, 16). Both TGFbeta 1 and TGFbeta 2 induce cataractous changes, TGFbeta 2 being the more potent isoform (16). The cataractogenic effects of TGFbeta are blocked by a pan-specific antibody against TGFbeta (17), and are not simply a "growth factor" effect. Weanling lenses cultured with another growth factor, FGF-2 (or FGF-1), at concentrations known to induce lens cell proliferation and/or fiber differentiation (18) remain transparent (Hales, A., C. Chamberlain, J. McAvoy, unpublished data).

In cultured lenses from weanling rats, opacities induced by TGFbeta correspond with subcapsular plaques of aberrant cells including spindle-shaped cells which are often associated with wrinkling of the lens capsule. Abnormal extracellular matrix deposition also occurs, mainly in the plaques. All these changes are typical of anterior and posterior subcapsular cataract and aftercataract (19). In addition, TGFbeta induces the accumulation of alpha -smooth muscle actin and type I collagen in both explants and cultured lenses (9, 10). These proteins, which are not generally found in the lens, are present in human anterior subcapsular cataract and in aftercataract (23). The present study shows that in lenses from adult rats, as for weanlings, TGFbeta induces opacities with morphological and molecular features of cataract. In each case, the plaques associated with the opacities are morphologically indistinguishable from early stage human anterior subcapsular cataracts (11).

Major findings of the present study are that the ovarian hormone, estrogen, protects rat lenses against TGFbeta -induced cataract, and that susceptibility to the cataractous changes induced by TGFbeta is sex dependent. Culturing lenses from ovariectomized females with TGFbeta resulted in marked opacification of the lens. Estrogen replacement in vivo prevented this response, but progesterone replacement did not (Table 2; Fig. 2). Furthermore, lenses from male rats were found to be more susceptible to the cataractogenic effects of TGFbeta than those from normal females (Table 1; Fig. 1), and lenses from normal rats of either sex seemed more resistant to the effects of TGFbeta than those from ovariectomized rats (Tables 1 and 2). The latter results are also consistent with a protective role for estrogen since circulating estrogens are present in male rats, albeit at much lower levels than in normal females (27), but not in ovariectomized females (28). Further support comes from a recent study involving transgenic mice. Females expressing a mutant, "nonresponsive" estrogen receptor progressively develop cortical and nuclear cataracts (29). It seems unlikely that the greater susceptibility of male rats to the cataractogenic effects of TGFbeta is due to the presence of testosterone since, in aging males, cataract increases (5) while testosterone decreases (30).

Sex-dependent and estrogen-related differences in susceptibility to cataract formation, consistent with a protective role for estrogen, have been noted in epidemiological studies. After menopause, the prevalence of cortical cataracts in females suddenly increases relative to males of equivalent age (2, 3). Before menopause, the prevalence of cataract seems to be similar in males and females (3, 31). In addition, the prevalence and severity of certain forms of cataract is lower in postmenopausal women on hormone replacement therapy involving administration of estrogen with or without progesterone, than in those who are not undergoing hormone replacement. This is true whether menopause occurs naturally or as a result of hysterectomy (5). Delayed menopause also seems to protect against cataract (5). Cernea et al. have suggested a possible link between presenile cataract development and ovarian hormone insufficiency (32). Furthermore, analysis of the age of patients who undergo cataract surgery indicates that men tend to develop presenile cataracts approximately four years earlier than women (2). The present study of cultured rat lenses provides strong support for this epidemiological evidence that estrogen protects against cataract.

This study goes beyond previous studies, however, in that it provides direct evidence that estrogen protects against cataract. Furthermore, it shows that estrogen does so by protecting lens cells against the cataractogenic effects of TGFbeta . This protective effect is observed whether estrogen is administered in vivo or in vitro. Thus, it seems likely that estrogen confers protection against cataract by targeting lens cells directly when administered in vivo, although the possibility of additional indirect benefits is not excluded. In other cellular systems, estrogen has been shown to have a variety of effects on TGFbeta . It may suppress the effects of TGFbeta . For example, estrogen-induced tumorigenesis in the anterior pituitary of rats is accompanied by a loss of sensitivity to TGFbeta 1 in tumor cells and downregulation of the TGFbeta type II receptor (33). Similarly, treating rats with estrogen in vivo reduces TGFbeta binding in the uterus, possibly via downregulation of TGFbeta receptors (34). On the other hand, estrogen may enhance TGFbeta activity when included in vitro (35, 36) or have no effect (37).

The present study adds weight to an increasing body of evidence that hormone replacement therapy not only relieves acute menopausal symptoms, but also offers protection against various diseases (38). These include cardiovascular diseases such as myocardial infarction, atherosclerosis, and related hypertension and stroke, as well as osteoporosis and associated fractures (38). Ocular pressure decreases progressively in both women and men, undergoing treatment with estrogen (42). In addition, estrogen use dramatically reduces the risk of idiopathic macular hole formation, an ocular condition that is more common in untreated postmenopausal women than in men of the same age (43). Many postmenopausal women are now receiving estrogen replacement therapy, in conjunction with progesterone where appropriate, but it is not universally advocated or available. In 1994, it was reported that only 5-10% of menopausal women in the United States were receiving this treatment (38).

The opacities that form in rat lenses cultured with TGFbeta are anterior subcapsular cataracts. Marked similarities between TGFbeta -induced cataract and aftercataract have already been noted (8, 10). Evidence of subtle changes typical of posterior subcapsular cataract and cortical cataract is provided by the present study. We report for the first time that TGFbeta can induce migration of aberrant cells along the lens capsule towards the posterior pole (as in Fig. 4 A). A similar abnormal migration of nucleated cells along the posterior capsule is thought to be the basis of human posterior subcapsular cataract formation (20). Some evidence of TGFbeta -induced cataract-like change was also observed in the cortical fibers in the present study (Fig. 3 A).

TGFbeta is potentially available to lens cells in situ. TGFbeta and its mRNA have both been detected in the mammalian eye (44), and biologically active TGFbeta has been found in samples of the ocular media that bathe the lens obtained from human patients suffering from cataract (47). Large reservoirs of inactive TGFbeta are present in the ocular media (47, 49, 51, 52) which may become activated, e.g., after eye surgery (53). It is not clear what level of TGFbeta stimulation lens cells receive under normal conditions in situ, and little is known about factors that regulate its activity in the eye (17). Estrogens have been detected in the ocular media in untreated female and male rabbits, but it is not clear what levels of active hormone are present (27).

The present study provides further evidence that TGFbeta is involved in the aetiology of various forms of subcapsular cataract, and possibly cortical cataract. Our finding that estrogen, administered in vivo or in vitro, protects against cataract is consistent with epidemiological studies. Thus, as suggested by previous studies from this laboratory, the rat lens model appears to be a valid tool for investigating factors that influence cataractogenesis. The parallel between estrogen effects in the rat system and epidemiological evidence also raises the possibility that estrogen may provide protection against human cataract by influencing a TGFbeta mediated mechanism of cataractogenesis. This study represents another significant step towards elucidating the molecular basis of cataract, the foundation for developing new strategies for prevention or treatment of this debilitating disease.


Footnotes

Address correspondence to Dr. J.W. McAvoy, Department of Anatomy and Histology (F13), University of Sydney, Sydney, NSW, Australia 2006.

Received for publication 6 June 1996

   The authors wish to thank Dr. Tim Shaw and Ms. Maria Bucci for performing the ovariectomies and administering replacement hormones, and Dr. Rebecca Mason for providing phenol red-free medium and estradiol. We also wish to thank Roland Smith for his assistance with the photography.
   This work was supported by grants from the National Health & Medical Research Council of Australia, the National Eye Institute, Department of Health, Education and Welfare, USA (R01 EYO3177), and a University of Sydney Faculty of Medicine Postgraduate Scholarship to A.M. Hales.

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