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Brief Definitive Report |
Address correspondence to Reinhold Förster, Institute of Immunology, Hannover Medical School, Feodor-Lynen-Str. 21, 30625 Hannover, Germany. Phone: 49-511-5329721; Fax: 49-511-5329722; email: foerster.reinhold{at}mh-hannover.de
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Abstract |
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Key Words: gut IgA lamina propria CCL25 cell trafficking
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Introduction |
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In the intestine, antigens are sampled by DCs located in the epithelium or by specialized epithelia overlying Peyer's patches (PPs). In these follicle-associated epithelia, microfold cells nonspecifically sample antigens from the gut lumen and transport them to professional antigen-presenting cells located in the subepithelial dome (SED; reference 2). To elicit an immune response, these cells migrate into either the adjacent interfollicular T cell zone, the B cellrich follicles of PPs, or even into the draining mesenteric lymph node (MLN) to activate lymphocytes (3, 4). Some of the activated B cells start to proliferate and generate germinal centers within PPs or MLN, which have been identified as the places where affinity maturation and probably isotype switch from IgM to IgA occurs. However, more recently it has been shown that isotype switch of B220+ IgM+ cells at least in part occurs in the lamina propria under the influence of local stimuli (5). Most of the fully differentiated B cells leave PPs and MLN and migrate via the lymphatics and the thoracic duct into the blood and from there to the lamina propria of the small intestine.
It has been proposed that signaling through the chemokine receptor CCR9 might be an important factor that targets cells to the intestine (6, 7). The CCR9 ligand CCL25/TECK is expressed by epithelial cells of the small, but not the large, intestine. CCR9 is expressed on virtually all small intestinal T cells, and murine IgA-producing PCs from the spleen, PPs, and MLN have been shown to migrate toward CCL25 and CXCL12, a ligand for CXCR4 in vitro (810). Notably, PCs of IgG or IgM isotype do not respond to CCL25 but migrate toward CXCL12 and CXCL9 (9, 11, 12), suggesting that the differential expression of chemokine receptors targets PCs to their final destination depending on the isotype of immunoglobulins they produce. Furthermore, during the course of a memory response, CXCR3 and CXCR4 have been implicated in guiding plasma blasts to inflamed tissues or to the bone marrow, respectively (13). In this report, we provide in vivo evidence that CCR9 is crucial for the positioning of PCs to the small intestine.
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Materials and Methods |
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Cells were stained using the following antibodies: anti-CD3-PE (Caltag), IgA-biotin, CD19-biotin (Biosource International), CD138-PE, B220-PerCP, IgM, and IgD (BD Biosciences). To stain cytoplasmatic IgA, cells were fixed for 20 min in 2% PFA in PBS on ice, washed, and resuspended for 20 min in 0.1% saponin in PBS.
Generation of Monoclonal CCR9 Antibody.
A peptide comprising amino acids 322 of mouse CCR9 was synthesized and coupled to KLH or OVA. Rats were immunized subcutaneously and intraperitoneally with a mixture of 50 µg peptide-KLH, 5 nmol CPG oligonucleotide (Tib Molbiol), 500 µl PBS and 500 µl IFA as described previously (15). Supernatants were tested by a differential ELISA and analyzed by flow cytometry using thymocytes derived from wild-type and CCR9-deficient mice.
Immunofluorescence.
Immunohistological analysis of adult PPs and MLN was done on cryosections as described previously (16). For detection of CXCR4 (clone 2B11) and CCR9 (clone 7E7, IgG2b), sections were blocked with mouse serum, incubated with hybridoma supernatants, and detected using mouse antirat Cy3 antibodies (Jackson ImmunoResearch Laboratories).
In Vivo Migration of BrdU-labeled Cells.
To label proliferating cells in vivo, wild-type and CCR9-deficient animals were injected intraperitoneally with 120 mg/kg BrdU (Sigma-Aldrich) in PBS 1 h before killing. Cells were isolated from MLN and PPs, and a total of 108 cells was injected intravenously into wild-type recipients. After 16 h, mice were killed, and the small intestine was embedded in paraffin using standard procedures. Sections were dewaxed, and BrdU-incorporated cells were detected using the BrdU staining kit (Oncogene Research Products) and Cy3-tyramid (NEN Life Science Products). IgA+ cells were identified using anti-IgA FITC antibody (Caltag).
Oral OVA Immunizations and Serum Ig Analysis.
ELISA assays for total serum Ig levels were performed as described previously (16). Biotinylated anti-Ig antibodies were purchased from BD Biosciences (anti-IgM, anti-IgG1, anti-IgG2a, anti-IgG2b, and anti-IgG3) and Biosource International (anti-IgA).
10 mice per genotype were gavaged with 2.5 mg OVA and 10 µg cholera toxin (CT) six times at 10-d intervals. For detection of OVA-specific IgA and IgM antibodies, plates were coated overnight with 5 µg/ml OVA, and appropriate dilutions of serum samples were added (1:1001:6,400). AntiIgA-biotin and antiIgM-biotin followed by streptavidin-peroxidase and 5-thio-2-nitrobenzoic acid were used for detection.
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Results and Discussion |
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These observations were confirmed applying the CCR9 mAb in immunohistology. In contrast to IgA+ cells of MLN and PPs, which showed high levels of CCR9 (Fig. 1 D and not depicted), the majority of IgA+ PCs of the small intestine showed weak or undetectable staining with the anti-CCR9 mAb (Fig. 1 E; compare with staining intensity shown in Fig. 1 D). In addition, cells staining positive for CCR9 seem to represent T cells (Fig. 1 E). Interestingly, another chemokine receptor, CXCR4, was readily detectable on all IgA+ PCs of all lymphoid organs examined, including PPs, MLN, and the small intestine (Fig. 1 F and not depicted). Because IgA+ PCs are known to readily leave the place where they have been generated (i.e., the secondary lymphoid organs), high expression of CCR9 appears to be specific for newly generated PCs, whereas this receptor becomes down-regulated once these cells reached their final destination. These data suggest that high levels of CCR9 are required for the homing of newly generated IgA+ PCs to the intestine, whereas this receptor seems to be dispensable for retaining PCs within this compartment. Because CXCR4 remains expressed on resident IgA+ PCs within the intestine, it seems possible that CXCR4 participates in this process. Indeed, data derived from CXCR4-deficient mice suggest a role for this chemokine receptor in retaining PCs within lymphoid organs such as bone marrow (11).
Reduced Numbers of PCs in the Intestinal Lamina Propria of CCR9 Mutant Mice.
Based on these findings, we compared the PC populations of wild-type and CCR9 mutant mice by counting the number of IgA+ PCs on cryosections of the small intestine (Fig. 2, A and B). In this paper, only villi were counted that were cut (judged on the analysis of serial sections) through the core of the villus. In wild-type animals, an average of 20.6 ± 1.2 (mean ± SEM) IgA+ cells per villus section was found. In contrast, in CCR9 mutant mice, the number of PCs per villus section was severely reduced to 11 ± 1.1 (mean ± SEM) cells per villus section (Fig. 2 C). As an internal control, the number of IgA- DAPI+ LPCs was determined on the same sections, revealing that CCR9 deficiency does not affect cell types other than IgA+ PCs in this compartment (Fig. 2 C, 19 ± 0.9 cells vs. 22 ± 1.6 cells). These results could also be confirmed by flow cytometry on permeabilized LPCs using an anti-IgA mAb that revealed a reduction of IgA+ PC numbers by 50% in CCR9 mutants (unpublished data).
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Interestingly, in wild-type mice, most of the PCs locate to the lower half of the villus (Fig. 2 C), which is the region where peak levels of CCL25 expression by the abutting epithelial cells have been described previously (8), further supporting the hypothesis that CCL25 signaling via CCR9 contributes considerably to the efficient homing to the small intestine. Most notably, no differences in the number of IgA+ PCs were identified in the large intestine of CCR9 mutants (wild type: 1 PC/4699 ± 255 µm2; CCR9 deficient: 1 PC/4832 ± 331 µm2; n = 5 animals for each group). This observation is also in line with the reported absence of CCL25 in the large intestine (8).
Reduced Homing Capacity of CCR9-deficient IgA+ PCs.
More than two decades ago, McDermott et al. identified proliferating cells in MLN and PPs, but not in peripheral LN, that were able to migrate into mucosal tissues and give rise to IgA-secreting cells (17). To directly address the function of CCR9 in this process, we labeled proliferating cells using BrdU and isolated them from MLN and PPs of wild-type and CCR9-deficient mice. 108 cells were injected i.v. into wild-type recipients, and after 16 h, the numbers of BrdU+ IgA+ PCs in the small intestine had been determined. Interestingly, PCs from wild-type mice were threefold more efficient in migrating into the small intestine compared with cells derived from CCR9-deficient mice (Fig. 3 A). These results contributed further weight to the idea that CCR9 is required for efficient migration of newly formed PCs into the small intestine.
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Although the total amount of serum IgA was found to be unchanged between wild-type and mutant mice, we were interested to know whether differences exist between CCR9 mutants and wild-type mice in the induction of antigen-specific IgA response after oral immunization with a T celldependent antigen. To this end, wild-type mice and CCR9 mutants were gavaged with 2.5 mg OVA and 10 µg CT at 10-d intervals. Serum levels of OVA-specific IgA were determined 9 d after each single OVA application. In wild-type animals, significant OVA-specific IgA levels were detectable after three OVA applications that further increased after subsequent applications of the antigen (Fig. 3 B). Interestingly, in CCR9 mutants, a barely detectable increase in OVA-specific IgA titers could be determined within the period of time analyzed, suggesting a severe impairment in the production of OVA-specific IgA in these animals (Fig. 3 B).
Unaltered Architecture and Cell Composition of MLN and PPs in CCR9-deficient Mice.
Because it is assumed that the induction of an IgA-specific antibody response after oral application of antigen plus CT requires antigen presentation within morphologically intact PPs and MLN, we further analyzed both organs. We used immunohistology and flow cytometry to identify possible alterations in cellular composition or architecture of both organs in CCR9 mutants that contained normal numbers of B and T cells, and both cell types were located in their appropriate microenvironments. In addition, PPs contained normal numbers of CD11c+, CD11b-, and CD11c+CD11b+ DCs (unpublished data). Recently, it has been described that DCs of the SED can be labeled and their path subsequently followed using fluorescent latex beads (4). Because we found CCR9-expressing cells, including DC within the SED (unpublished data), we tested whether the mobilization of DCs of the SED is affected in CCR9 mutants. Wild-type and CCR9-deficient mice were deprived of water and food for 4 h and subsequently gavaged with 1012 fluorescent latex beads (200 nm diameter; Polysciences) per animal. After 24 h, the mice were gavaged with 50 µg of CT. After another 24 h, PPs and MLN were sectioned and stained for CD11c and CD3 (Fig. 4, AD, PPs only). In wild type and CCR9 mutants, application of CT lead to reduced numbers of CD11c+ cells (red) present in the SED (Fig. 4, AD). To quantify this effect, the absolute number of fluorescent beads in the SED was counted in 20 PPs derived from four wild type and four CCR9 mutants. In both wild type and CCR9 mutants, CT administration triggers an identical fivefold decrease of bead-labeled DCs localized to the SED, suggesting that no differences in DC mobilization exist and that the activation of naive B cells should proceed normally in these CCR9-/- organs (unpublished data). Indeed, analysis of cryosections from MLN of wild-type and mutant mice that were immunized five times with OVA and CT revealed comparable numbers of IgA+ PCs in both strains (Fig. 4, E and F). Furthermore, in wild type and mutants likewise, the total numbers of IgA+ PCs strongly increased after immunization in comparison to nonimmunized mice (unpublished data) strongly supporting the idea that the generation of IgA PCs is not affected by CCR9 deficiency.
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One hypothesis accounting for these defects would postulate the existence of a rather uniform PC population inside the small intestine. In this scenario, freshly generated plasma blasts that express high levels of CCR9 gain efficient entry into the intestinal lamina propria by CCL25-mediated signaling, whereas the residual PC population in CCR9-deficient mice would enter the lamina propria independent of CCR9, probably by a time-consuming and inefficient bypass mechanism.
Alternatively, dependence on CCR9 signaling might reflect the different developmental fate and origin of PCs populating the small intestine. It has been suggested that besides conventional B2 cells, B1 cells from the peritoneal cavity can give rise to lamina propria IgA+ PCs (18). However, this issue remains discussed controversially because intestinal IgA production in conventionally reared animals (as performed in this work) has been shown to be the source of almost exclusively B2, but not B1, cells (19). Unfortunately, available immunological tools would not allow dissection of B1 and B2 cellderived PCs once they settled the lamina propria. Interestingly, intraperitoneal immunization with T cellindependent antigen results in a normal antigen-specific IgM response in CCR9 mutants, suggesting that B1 cell function per se is not affected in these animals (unpublished data). Thus, a more likely explanation for the decrease of IgA+ PCs in CCR9 mutants is the less efficient recruitment of B2 cellderived PCs into the lamina propria in the absence of CCR9 signaling. This idea is consistent with a strongly reduced IgA response in orally immunized mice assuming that antigen-specific PCs are generated in CCR9 mutants but fail to reach a compartment that supports secretion and long-term survival of these cells.
In any case, CCR9-independent chemokine receptor signaling would be required to guide the remaining PCs into the lamina propria in CCR9-deficient mice, resulting in serum and fecal IgA levels that do not significantly differ from those present in wild-type animals (unpublished data). Indeed, surface expression of CXCR4 can be detected on IgA+ PCs (Fig. 1 F) consistent with the responsiveness of these cells to CXCL12 in in vitro migration assays (9). Furthermore, in humans, CCR10 has been documented in PCs, including intestinal IgA+-secreting PCs (20), and we were able to detect CCR10 expression in mouse PCs using a CCL27-IgG1 fusion protein (unpublished data) suggesting that CCR9 and CCR10 might cooperate in directing PCs into the small intestine in mice. In conclusion, our findings represent the first in vivo evidence that CCR9 is critical for the homing of IgA-secreting PCs to the small intestine.
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Acknowledgments |
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This work was supported by a Deutsche Forschungsgemeinschaft grants (SFB621-A1 and Fo334/1-1) to R. Förster and by institutional grants from the Institut National de la Santé et de la Recherche Medicale, the Centre National de la Recherche Scientifique, and a specific grant from the European Community (QLG1-CT1999-00202).
Submitted: 19 June 2003
Accepted: 26 November 2003
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References |
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