The Use of Lymphocyte Function–associated Antigen (LFA)-1–deficient Mice to Determine the Role of LFA-1, Mac-1, and {alpha}4 Integrin in the Inflammatory Response of Neutrophils

Robert B. Hendersona, Lina H.K. Limb, Philippe A. Tessiera, Felicity N.E. Gavinsb, Meg Mathiesa, Mauro Perrettib, and Nancy Hogga
a Leukocyte Adhesion Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX, UK
b Department of Biochemical Pharmacology, William Harvey Research Institute, London EC1M 6BQ, UK

Correspondence to: Nancy Hogg, Leukocyte Adhesion Laboratory, Imperial Cancer Research Fund, London WC2A 3PX, UK. Tel:44-20-7269-3255 Fax:44-20-7269-3093 E-mail:hogg{at}icrf.icnet.uk.


  Abstract
Top
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgements
References

After injury or infection, neutrophils rapidly migrate from the circulation into tissues by means of an orderly progression of adhesion receptor engagements. Neutrophils have been previously considered to use selectins exclusively to roll on vessels before an adhesion step mediated by the ß2 integrins, lymphocyte function–associated antigen (LFA)-1, and Mac-1. Here we use LFA-1-/- mice, function blocking monoclonal antibodies, and intravital microscopy to investigate the roles of LFA-1, Mac-1, and {alpha}4 integrins in neutrophil recruitment in vivo. For the first time, we show that LFA-1 makes a contribution to neutrophil rolling by stabilizing the transient attachment or tethering phase of rolling. In contrast, Mac-1 does not appear to be important for either rolling or firm adhesion, but instead contributes to emigration from the vessel. Blocking Mac-1 in the presence of LFA-1 significantly reduces emigration, suggesting cooperation between these two integrins. Low levels of {alpha}4ß1 integrin can be detected on neutrophils from LFA-1+/+ and -/- mice. These cells make use of {alpha}4ß1 during the rolling phase, particularly in the absence of LFA-1. Thus LFA-1 and {alpha}4ß1, together with the selectins, are involved in the rolling phase of neutrophil recruitment, and, in turn, affect the later stages of the transmigration event.

Key Words: LFA-1, integrin, rolling, neutrophils, inflammation


  Introduction
Top
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgements
References

Neutrophils are the first arrivals at sites of inflammation, responding actively to signals produced by the vasculature early in an immune response (1). To migrate from the circulation across postcapillary venules and into tissues, neutrophils and other leukocytes make use of selectin and integrin adhesion receptors in a sequence of several overlapping steps. The current model is that neutrophil velocity is decreased through the selectins, enabling them to roll along the vessel wall. Endothelial cell–bound factors, such as platelet-activating factor or chemokines, then cause activation of neutrophil CD18 (ß2) integrins. Using their activated integrins, neutrophils flatten and move along the endothelium before migrating across the blood vessel wall into the surrounding tissue along a gradient of chemotactic factors.

Previously, neutrophils have been thought to express ß2 but not ß1 (CD29) integrins, although more recently {alpha}4 integrins have been identified on these leukocytes (for a review, see reference (2)). However, it is the two main ß2 integrins, lymphocyte function–associated antigen (LFA)-1 (CD11a/CD18) and Mac-1 (CD11b/CD18), which neutrophils are reported to utilize in models of inflammation in human (3) (4), rabbit (5), rat (6), and mouse (for a review, see references (7) and (8)). Questions remain as to their individual roles. In one model, Mac-1 was shown to be the predominant ß2 integrin used during neutrophil migration into the peritoneum (3) (9). In further studies, LFA-1 was identified as the principal neutrophil integrin used during transendothelial migration (5) (10). The use of mice deficient in the ß2 integrins Mac-1 or LFA-1 has suggested a division of function between these two integrins. LFA-1 is considered chiefly to control transmigration with Mac-1 involved in other neutrophil functions, such as apoptosis, degranulation, and iC3b-mediated phagocytosis (10) (11) (12) (13) (14). Confusingly, in studies with the CD18-/- mouse, CD18 integrins were reported to have no role in response to lung or peritoneal inflammation (15). These variable results have been attributed to species differences, the effects of integrin deficiency on circulating leukocyte numbers, and differences in the inflammatory agents and models employed.

We have examined the respective roles of LFA-1, Mac-1, and {alpha}4 integrin in neutrophil migration into the inflamed peritoneum. To further define the roles of these three integrins in neutrophil behavior, in this model we have compared the responses of LFA-1-/- mice with their wild-type counterparts in the presence and absence of function blocking mAbs. In addition, intravital microscopy has been used to further clarify the functions of the individual leukocyte integrins.


  Materials and Methods
Top
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgements
References

LFA-1-/- and Littermate+/+ Mice.
LFA-1-/- and +/+ mice were derived and maintained as described previously (16). Sex-matched, 10–14-wk-old mice were used in the experiments. All animal work was performed according to UK Government Home Office Regulations (1986).

Preparation of mAbs and Fab Fragments.
The following purified rat mAbs were used to block integrin function in this study: H68 (LFA-1, CD11a; IgG2a), PS2/3 ({alpha}4 integrin, CD49d; IgG2b), and 5C6 (Mac-1, CD11b; IgG2b; reference (9)) (obtained from Dr. Siamon Gordon, Oxford University, Oxford, UK). Purified mAbs H68, PS2/3, and 5C6, plus control mAbs PyLT-1 (IgG2b) or YB238 (IgG2a), were prepared by the Imperial Cancer Research Fund Research Monoclonal Antibody Service. Fab fragments were generated as specified by the manufacturer using the ImmunoPure® Fab kit (Pierce Chemical Co.), purified by using a protein G Sepharose column, and tested for purity by SDS-PAGE electrophoresis. All mAb IgGs and Fabs were assessed for denaturation/aggregation by SDS-PAGE and flow cytometry using murine PBLs.

Flow Cytometric Analysis.
Mice were tail bled 4 h after induction of peritonitis. Blood was diluted 1:5 in PBS, 1.5% dextran T500 (Amersham Pharmacia Biotech), and RBCs were sedimented for 30 min. Unsedimented white blood cells were further isolated on Histopaque 1119 (Sigma-Aldrich) according to the manufacturer's instructions.

Lavage and blood cells were centrifuged (500 g for 10 min at 4°C) and resuspended in FACSwashTM (PBS, 0.2% BSA, and 1% normal goat serum). Samples of 0.5–1 x 106 cells were treated for 30 min with 10 µg/ml mAb 2.4G2 (BD PharMingen), in order to block Fc{gamma} II/III receptors. Cells were then stained with the following mAbs at an optimal concentrations (2–10 µg/ml) for 25 min at 4°C followed by three washes in FACSwashTM. The following mAbs are unconjugated (Imperial Cancer Research Fund) or directly conjugated/biotinylated (BD PharMingen) and specific for: LFA-1 (H68), Mac-1 (5C6 and M1/70-biotin), L-selectin (Mel-14-biotin), {alpha}4 integrin (PS2/3, R1-2-FITC, 9C10-biotin), IgG2b isotype (PyLT-1, A95-1-biotin, A95-1-FITC), and IgG2a isotype (R35-95-biotin, YB238). Unconjugated and biotinylated mAb binding to cells was detected by incubation with FITC-conjugated goat anti–rat IgG (1:200; Jackson ImmunoResearch Laboratories) or tricolor-streptavidin (1:200; Caltag), respectively. Neutrophils were identified on the basis of subsequent staining with 7/4-PE (Serotec) and/or Gr-1(RB6-8C5-FITC) in conjunction with scatter profiles. After three washes, the cells were resuspended in PBS containing 2% formaldehyde and cell fluorescence was measured on a FACSCaliburTM (Becton Dickinson).

Peritoneal Inflammation.
Peritonitis was induced by intraperitoneal injections of thioglycollate (TG) (3% wt/vol in 0.5-ml sterile saline; Sigma-Aldrich). 4 h later, the mice were euthanized by carbon-dioxide exposure, and peritoneal cavities were lavaged with 5 ml PBS containing 3 mM EDTA and 25 U/ml monoparin (CP Pharmaceuticals). Leukocytes were counted and the proportion of neutrophils determined by mAb 7/4 staining and flow cytometry. Alternatively, leukocytes were stained with Turk's solution (0.01% crystal violet in 3% acetic acid) and neutrophils were counted. In peritonitis and intravital experiments, either 100 µg of purified Fab (all intravital experiments) or 130 µg of intact mAb diluted in PBS was injected intravenously into the tail vein 15 min before the TG injection. Over the course of the 4-h experiment, this amount of mAb fully saturated the circulating neutrophils and, where tested, the lavage neutrophils (data not shown).

Intravital Microscopic Studies.
4 h after TG injection, the mesenteric vascular bed was prepared for microscopy (17). Mice were anesthetized with diazepam (60 mg/kg subcutaneously) and hypnormTM (0.7 mg/kg fentanyl citrate and 20 mg/kg fluanisone, intramuscularly). Cautery incisions were made along the abdominal region and the mesentery vascular bed was exteriorized and placed on a viewing plexiglass stage. The preparation was mounted on a Zeiss Axioskop "FS" (original magnification: 40x) to observe the microcirculation and transilluminated with a 12-V, 100-W halogen light source. Mesenteries were superfused with bicarbonate-buffered solution (g/liter: 7.71 NaCl, 0.25 KCl, 0.14 MgSO4, 1.51 NaHCO3, and 0.22 CaCl2, pH 7.4, at 37°C, gassed with 5% CO2/95% N2) at a rate of 2 ml/min. A Hitachi CCD color camera (KPC571) acquired images which were displayed on a Sony Trinitron color video monitor (PVM 1440QM) and recorded on a Sony super VHS video cassette recorder (SVO-9500 MDP) for subsequent offline analysis. A video time-date generator (FOR.A video timer, VTG-33) projected the time, date, and stopwatch function onto the monitor.

Wall shear rate (SR) was calculated by the Newtonian definition: SR = 8,000x (Vmean/diameter) and expressed in s-1. One to three randomly selected postcapillary venules (diameter between 20–40 µm; length of at least 100 µm) were observed for each mouse. White blood cell velocity in millimeters per second was calculated from time taken for a leukocyte to roll along 100 mm of vessel wall. Cell flux was calculated by counting, in the vessel, the number of white blood cells passing through a theoretical 90° plane in a 5-min period. Data are expressed as cells per min. Cell adhesion for each vessel was quantified by counting the number of adherent neutrophils in a 100-µm length. Leukocytes stationary for at least 30 s were defined as adherent. Leukocyte emigration from the microcirculation into the tissue was measured by counting the number of leukocytes that had emigrated up to 50 mm away from the wall per 100 µm-vessel segment.

Statistics.
Statistics were calculated on original values by using analysis of variance (ANOVA) followed by the Bonferroni test for intergroup comparisons. The alternate Student's t test was used when two groups were analyzed. In all cases a threshold value of P < 0.05 was considered as the minimum level of significance.


  Results and Discussion
Top
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgements
References

Neutrophil Migration into the Peritoneum After an Inflammatory Stimulus.
To assess the role of LFA-1 in neutrophil migration to extravascular sites in response to an inflammatory stimulus, LFA-1-/- and +/+ mice were compared using the TG-induced peritonitis model. Mice were killed at 0, 4, 10, 16, and 24 h after injection and neutrophil emigration into the peritoneal cavity was quantified. The migration of neutrophils was evident at 4 h, and reached a maximal level at 16 h which was maintained until 24 h (data not shown). At the 4-h time point, 50% fewer neutrophils migrated in the LFA-1-/- mouse compared with the LFA-1+/+ (Fig 1). As circulating blood neutrophils were in higher concentration in the LFA-1-/- mice (4.4 ± 0.5 x 106 cells per milliliter (n = 9) as compared with 0.8 ± 0.1 x 106 cells per milliliter (n = 12) in the LFA-1+/+ mice), this represents an ~10-fold greater efficiency of migration by the wild-type LFA-1 neutrophils. This finding is in agreement with previous studies in mouse (10), rat (6), and rabbit (5), in which a function blocking LFA-1 mAb substantially inhibited neutrophil migration. Other studies making use of LFA-1-/- mice also report similar results (11) (12) (13) (14).




View larger version (42K):
[in this window]
[in a new window]
 
Figure 1. The migration of neutrophils at 4 h after TG treatment into the peritoneum of LFA-1+/+ and -/- mice in the presence of mAbs specific for LFA-1, Mac-1, and {alpha}4 integrins either singly or in combination. For LFA-1+/+ mice, anti–LFA-1 and anti–Mac-1 mAbs, when administered alone, significantly inhibit neutrophil migration. All mAb pairs were significantly effective in inhibiting migration compared with the control. In addition, all pairs of mAbs block significantly more than single mAbs (P < 0.001), indicating that {alpha}4 integrin cooperates with LFA-1 and Mac-1 in the migration of LFA-1+/+ neutrophils. For LFA-1-/- mice, only anti-{alpha}4 integrin inhibits neutrophil migration as a single mAb and does not inhibit more significantly when in combination with anti–Mac-1 mAb ({alpha}4 versus {alpha}4/Mac-1 mAbs; P > 0.05). These data are representative of three complete experiments which utilized 3–10 mice per condition per experiment. Data are reported as mean ± SEM.

Integrins Involved in Neutrophil Transmigration in LFA-1-/- Versus LFA-1+/+ Mice.
To investigate which integrins have a role in the migration of neutrophils into the peritoneum, LFA-1-/- and +/+ mice were injected intravenously with anti–LFA-1, Mac-1, or {alpha}4 integrin mAb 15 min before the injection of TG into the peritoneal cavity. In LFA-1+/+ mice, the anti–LFA-1 and the anti–Mac-1 mAbs significantly inhibited neutrophil migration (Fig 1 A). The effect of the {alpha}4 mAb was less pronounced. When the mAbs were tested in pairs, each combination caused a further decrease in neutrophil emigration compared with the single mAbs. This suggests that all three integrins cooperate in the movement of neutrophils from the circulation to the final destination of the peritoneum, with LFA-1 and Mac-1 having dominant roles.

As expected, anti–LFA-1 mAb was ineffective at preventing the migration of neutrophils in LFA-1-/- mice in response to the TG injection (Fig 1 B). Surprisingly, anti–Mac-1 alone was also ineffective in blocking migration. However, anti-{alpha}4 mAb significantly blocked migration. There was no further significant difference between {alpha}4 mAb alone or when coinjected with anti–Mac-1 mAb (P > 0.05). Similar results were obtained in both LFA-1+/+ and -/- mice with Fab fragments (data not shown). These results suggest that, while normal neutrophils make use of Mac-1, LFA-1, and {alpha}4 integrin to access the peritoneal environment, the LFA-1–deficient neutrophils emigrate using {alpha}4 integrin with a less significant role for Mac-1.

Phenotypic Characteristics of LFA-1-/- and +/+ Neutrophils in the Circulation and Peritoneum.
Since the LFA-1-/- neutrophils were able to migrate into sites of inflammation by making use of {alpha}4 integrin, it was of interest to compare the expression of this integrin, as well as Mac-1, on both LFA-1+/+ and -/- neutrophils. We also wished to determine whether integrin expression was altered between circulating and emigrated neutrophils. As expected, expression of the ß2 integrin LFA-1 was absent on neutrophils from LFA-1-/- mice (data not shown). The levels of Mac-1 were similar on peripheral blood neutrophils both before (Fig 2 A) and after TG stimulation (data not shown). However, expression of Mac-1 was increased on peritoneal neutrophils compared with peripheral blood neutrophils and this upregulation occurred to a greater degree in LFA-1–deficient mice. As neutrophils with this higher level of expression of Mac-1 are not represented in the circulating pool at 4 h after TG, Mac-1 must be upregulated after contact with the endothelium or subendothelial tissue matrix. This increase in Mac-1 expression does not account for the lack of blocking effect of anti–Mac-1 mAb on LFA-1-/- neutrophil migration (Fig 1) as levels of injected mAbs were saturating on both circulating and peritoneal lavage neutrophils (n = 2 experiments; data not shown).




View larger version (71K):
[in this window]
[in a new window]
 
Figure 2. Flow cytometric analysis of LFA-1+/+ and -/- neutrophils in the circulation and after extravasation into the peritoneum. Expression of (A) Mac-1 detected with mAb M1/70 biotin and (B) {alpha}4 integrin detected with mAb 9C10-biotin. Geometric means of the major peak are shown in the top right hand corner. Data are representative of five experiments with blood and lavage cells pooled from five and two mice, respectively.

{alpha}4 integrin was expressed on circulating neutrophils, with no further increase on lavage neutrophils (Fig 2 B). Detection of {alpha}4 integrin with three specific mAbs paired with matched isotype controls and with prior blocking of FcR (Materials and Methods), gives confidence that the neutrophils under study do express this class of integrin. Positive staining with {alpha}4 and ß1 mAbs, but not ß7- nor {alpha}4ß7-specific mAbs indicated that the {alpha}4 integrin expressed by neutrophils was {alpha}4ß1 rather than {alpha}4ß7 (data not shown). Unlike Mac-1 expression there was no discernible difference in expression of {alpha}4ß1 on the lavage cells of LFA-1+/+ and -/- mice (Fig 2 B). A subset comprising ~10% of neutrophils expressed higher levels of {alpha}4ß1. These cells may represent less mature bone marrow–derived neutrophils (18) as characteristic mAb staining and scatter properties distinguished these cells from monocytes (intermediate mAb Gr-1 expression) and eosinophils (no mAb Gr-1 staining and distinctive high side scatter; data not shown). The expression of {alpha}4 integrin by neutrophils has been controversial because flow cytometric studies have suggested that, unlike other leukocytes, neutrophils express only ß2 integrins. However, recent studies in rodents (for a review, see reference (2)) and now functional studies in humans (4) have provided evidence for {alpha}4 integrin expression and use by neutrophils.

The Use of Intravital Microscopy to Examine LFA-1+/+ and LFA-1-/- Neutrophils in Response to TG Treatment.
Leukocytes migrate across the postcapillary venules of the mesentery after introduction of a stimulant into the peritoneum. Intravital microscopy was used to investigate whether the absence of LFA-1 had any effect on the rolling of the leukocytes along the mesenteric venules. Additionally, it allowed assessment of the importance of LFA-1 in adhesion to, as well as emigration from, the mesenteric venules. In LFA-1+/+ mice, the leukocytes rolled with a velocity of 9.7 ± 1.2 µm/s after 4 h of exposure to TG (Fig 3 A). For the LFA-1-/- mice, the velocity of leukocyte rolling was significantly increased to 32.1 ± 8.9 µm/s, indicating that LFA-1 participates in rolling along the mesenteric vessels after an inflammatory stimulus. Histology of stimulated mesenteric vessels showed the rolling cells to be neutrophils (unpublished data). The velocity of neutrophil rolling is dependent not only on the activity of adhesion receptors on both the leukocyte and the endothelium, but also on the diameter of the postcapillary venules and the SR at the vessel wall. The velocity increase observed could be attributed to adhesion receptor expression as there was no significant difference in these two parameters in LFA-1-/- and +/+ vessels (Table 1).






View larger version (60K):
[in this window]
[in a new window]
 
Figure 3. The effect of LFA-1 deficiency on neutrophil rolling, adhesion, and transmigration as detected by intravital microscopy. A comparison in LFA-1+/+ and -/- mice of leukocyte (A) velocity (VWBC); (B) integrated cell flux (i.e., cell flux per 106 leukocytes per milliliter of blood). Inset, basic cell flux (cells per min); (C) cell adhesion; (D) emigrated leukocytes per field ("field" represents cells within 50-µm distance from a 100-µm segment of vessel). The neutrophils from LFA-1-/- mice have a higher velocity than those from LFA-1+/+ mice indicating a role for LFA-1 in neutrophil rolling. Because LFA-1 does not alter the cell flux (frequency of contact with endothelium), it must be decreasing the velocity by transiently stabilizing the rolling cells. A lack of LFA-1 is also reflected in lower adhesion per 100-µm length of mesenteric vessel and decreased emigration from the vasculature by the LFA-1-/- as compared with LFA-1+/+ neutrophils. Data are reported as mean ± SEM.


 
View this table:
[in this window]
[in a new window]
 
Table 1. Parameters Analyzed for Mesenteric Postcapillary Venules of LFA-1+/+ and LFA-1-/- Mice

Most previous reports have restricted the role of LFA-1 to mediating firm adhesion and this is the first indication that it can participate in vivo in the rolling event. The velocity of the rolling leukocyte will be influenced by changes in either the "on" or "off" rate of binding to the endothelium. To further define where LFA-1 was affecting the rolling velocity, we investigated the "cell flux "or frequency of neutrophil encounter with the endothelium and observed a substantially larger number of leukocytes transiently adhering to endothelium in the LFA-1-/- compared with the LFA-1+/+ mice (Fig 3 B, inset). However, when the increased numbers of leukocytes circulating in the LFA-1-/- mice were taken into account (Fig 3, legend), the frequency of encounter with endothelium per 106 leukocytes per milliliter was not significantly different between LFA-1+/+ and -/- mice (Fig 3 B). Considered together, these observations indicate that the lack of LFA-1 did not significantly affect the on or attachment phase of the rolling interaction. However, in the absence of LFA-1, the stability of the brief attachment or tethering phase of rolling was compromised resulting in a faster off rate.

These results suggest that during the rolling phase of the interaction with endothelium, LFA-1 may operate together with the selectins. We confirmed that expression levels of L-selectin on LFA-1+/+ and -/- neutrophils were similar, indicating that the effect on rolling was not indirectly caused by loss of L-selectin, but was due to the absence of LFA-1 (data not shown). In another in vivo study, ß2 integrin ligand, intercellular adhesion molecule (ICAM)-1, cooperated with L- and P-selectin in leukocyte rolling, supporting the idea that ß2 integrin is needed for optimal rolling–mediated by these two selectins (19). In contrast, two studies which have analyzed LFA-1 in the form of LFA-1–expressing K562 cells (20) or glycosylphosphatidylinositol-linked LFA-1 I domain (21) show that this integrin can mediate rolling on isolated ICAM-1 under shear conditions in vitro. This suggests that LFA-1 can act as an independent rolling receptor in some contexts. However, the findings here suggest that under in vivo conditions, in the presence of other receptors involved in rolling such as the selectins, LFA-1 has a cooperative role in providing transient stability for the rolling cells.

Marked differences were observed between the LFA-1+/+ and -/- mice at each step of the recruitment pathway. The increase in neutrophil rolling velocity had an impact on adhesion to and transmigration across mesenteric vessels, as both were reduced in LFA-1-/- mice compared with the LFA-1+/+ mice. The numbers of neutrophils adhering to (4.3 ± 0.8 vs. 6.9 ± 0.7) and emigrating from (3.4 ± 1.3 vs. 8.9 ± 0.7) the vessels of LFA-1-/- versus +/+ mice were lower (Fig 3c and Fig d). The overall conclusion using intravital microscopy is that the absence of LFA-1 considerably increases the neutrophil rolling velocity along the postcapillary venules after an inflammatory stimulus which, in turn, reduces both cell adhesion to and emigration from the venules.

Effect of Anti-Integrin mAbs on Neutrophil Velocity Using Intravital Microscopy.
We next utilized function blocking Abs to examine the involvement of LFA-1, as well as Mac-1 and {alpha}4ß1 in neutrophil rolling, adhesion, and transmigration. Investigation of LFA-1+/+ mice showed that the LFA-1 mAb significantly increased the velocity of neutrophil rolling (19.9 ± 2.3 vs. 9.6 ± 1.0 µm/s) as did the {alpha}4 mAb (17.7 ± 1.5 vs. 9.6 ± 1.0 µm/s) (Fig 4 A). When the LFA-1-/- mice were similarly treated with mAbs to the individual leukocyte integrins, the velocity was increased significantly only by the {alpha}4 mAb (46.0 ± 7.4 vs. 29.0 ± 6.8 mm/s). Together these results indicate that both LFA-1 and {alpha}4 integrin participate in the rolling velocity of neutrophils on the mesenteric vessels after TG stimulation. It appears that two pathways are in place with LFA-1+/+ neutrophils making use of LFA-1 and {alpha}4ß1 whereas, LFA-1-/- neutrophils are relying only on {alpha}4ß1. Mac-1 has no part in this stage of the response to TG.



View larger version (37K):
[in this window]
[in a new window]
 
Figure 4. The effect of mAbs specific for LFA-1, Mac-1, and {alpha}4 integrin on neutrophil rolling, adhesion, and migration in LFA-1+/+ and -/- mice. For LFA-1+/+ mice, anti–LFA-1 mAb significantly affected all three stages of rolling, adhesion, and emigration and anti-{alpha}4 mAb had significant impact on cell rolling and adhesion. Anti–Mac-1 mAb significantly affected the emigration step in LFA-1+/+ but not LFA-1-/- mice. In LFA-1-/- mice, only the {alpha}4 mAb significantly affects all three steps of rolling velocity, adhesion, and emigration. Data are reported as mean ± SEM.

This role for {alpha}4 integrins in neutrophil rolling is a new observation, but their participation in rolling and adhesion of eosinophils and other leukocytes has been well documented. The {alpha}4 integrins can mediate rolling of lymphocytes on the high endothelial venules of mucosal lymph nodes (22), of eosinophils on mesenteric vessels (23), and of murine hematopoietic progenitor cells on bone marrow microvessels (24). In fact, primary lymphocytes can use {alpha}4 integrin to roll on ligand, vascular cell adhesion molecule 1, which indicates that {alpha}4 integrin can act independently of the selectins as a rolling receptor (22) (25). In this context, the topological association of L-selectin and {alpha}4 integrin with neutrophil microvilli, compared with the restriction of LFA-1 to the neutrophil cell body, may have some bearing on their individual functions in the rolling phenomenon (22).

Effect of Anti-Integrin mAbs on Neutrophil Adherence Using Intravital Microscopy.
mAbs that increased the velocity of rolling also decreased the number of adherent leukocytes (Fig 4 B). For the LFA-1+/+ mice, the anti–LFA-1 mAb (2.7 ± 0.4 vs. 5.5 ± 0.4) and {alpha}4 mAb (4.2 ± 0.4 vs. 5.5 ± 0.4) both decreased adhesion to the TG-stimulated venule. Only the {alpha}4 mAb decreased the adhesion of the LFA-1-/- leukocytes (0.9 ± 0.4 vs. 3.1 ± 0.3). Another study investigating neutrophil adherence under physiological SRs in vitro showed that they can adhere via {alpha}4 integrin independently of ß2 integrins (26). Our results imply that where these integrins influence the velocity of rolling of the neutrophils, they also have impact on the firm adhesion to the same vasculature. A precedent for this conclusion comes from a shear flow model, in which, with subsecond timing, the chemokine stromal cell–derived factor 1 initiates {alpha}4 integrin–mediated T lymphocyte rolling along vascular cell adhesion molecule 1, and this rolling is then converted to arrest and firm adhesion (25). Since the process of leukocyte emigration is sequential, an effect on rolling would be expected to impact upon adhesion and ultimately transmigration.

Effect of Anti-Integrin mAbs on Neutrophil Emigration Using Intravital Microscopy.
Next the effect of blocking mAbs was tested on the emigration of leukocytes within a 50-µm distance per 100-µm length of vessel. For the LFA-1+/+ leukocytes, LFA-1 mAb-inhibited migration as was expected (2.7 ± 0.4 vs. 6.1 ± 1.0), but for the first time, mAb specific for the second ß2 integrin, Mac-1, also showed a significant effect (3.1 ± 0.3 vs. 6. 1± 1.0; Fig 4 C). The {alpha}4 mAb was the only mAb to significantly affect the numbers of emigrating cells in the LFA-1-/- mice (1.6 ± 0.2 vs. 3.4 ± 0.4).

Thus, we show that Mac-1 does cooperate with LFA-1 in the transmigration process, but the results challenge previous theories concerning where Mac-1 engagement occurs. In vitro studies measuring neutrophil adhesion to ICAM-1 under shear flow have suggested that cooperation occurs between LFA-1 and Mac-1 and that transient adhesion by LFA-1 promotes stable longer-lasting Mac-1–mediated adhesion (14) (27) (28). In this study, anti–Mac-1 mAb does not affect the phases of rolling or adhesion, but is effective at the level of emigration and consequently in the overall movement of neutrophils into the peritoneum (Fig 1). We reasoned that the Mac-1 mAb could either be affecting transmigration across the mesenteric vessel or blocking a postendothelial event such as migration within the tissue extracellular matrix. If the second option were correct, then it would be expected that leukocytes would accumulate perivascularly in the presence of the mAb. Comparison of the video recordings of control versus Mac-1 mAb-treated preparations revealed no difference in the concentration of leukocytes in the immediate perivascular location (data not shown). Therefore, these results suggest a role for Mac-1 in the migration of leukocytes specifically across the endothelium itself. Interestingly, Mac-1 does not have the same impact on the {alpha}4-mediated emigration of LFA-1-/- neutrophils, suggesting that {alpha}4ß1 can accomplish all the steps of the adhesion and transmigration process for LFA-1-/- neutrophils. Neutrophils from Mac-1-/- mice have enhanced migratory properties (10) (13) (14) in terms of their movement into inflammatory sites. Why their properties differ from neutrophils treated with anti–Mac-1 mAb is unclear.

In the inflammatory model used in this study we have shown that neutrophil LFA-1 can participate in rolling interactions with ligand on mesenteric vessels. This first stage then dictates the rest of the transmigration event. The fact that {alpha}4ß1 is the principal integrin used by the LFA-1-/- neutrophils to extravasate highlights the fact that neutrophils basically have two integrin-based pathways available for exiting the circulation and migrating across the endothelium. In some situations, LFA-1 can suppress {alpha}4 integrin activity through an integrin "cross-talk" mechanism. This cross-talk does not feature here, as {alpha}4ß1 contributes to the migratory response of LFA-1+/+ neutrophils, in combination with the other ß2 integrins LFA-1 and Mac-1. The {alpha}4ß1-mediated mechanism is not as efficient as that provided by LFA-1/Mac-1 as ~10-fold fewer neutrophils are seen to transmigrate early in the inflammatory response.

Intravital microscopy studies have identified previously a key role for L-selectin in neutrophil rolling on inflamed endothelium (29) with integrin engagement occurring only at a later stage after leukocyte activation. Here we suggest that the two processes are integrated, with LFA-1 acting earlier in the process, performing the task of providing stability to the selectin tether and slowing the velocity of the rolling neutrophil. Klaus Ley and colleagues (30) have suggested that this is a progressive event with increasing numbers of integrins engaged over time leading to leukocyte arrest and firm adhesion. In summary, the findings of this study indicate that the receptor repertoire controlling neutrophil rolling interactions with the inflamed endothelium is broader than considered previously (1) and includes LFA-1 and the {alpha}4ß1 integrins, further blurring the distinctions in function between the different classes of adhesion receptors.


  Footnotes

R.B. Henderson and L.H.K. Lim contributed equally to this work.


  Acknowledgements
Top
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgements
References

We thank our colleagues Caetano Reis e Sousa, Mike Owen, Josie Hobbs, Matthew Robinson, and Kiki Tanousis for their very helpful comments on this manuscript. We are grateful to Gill Hutchinson and Julie Bee for their expert technical assistance and Dave Ferguson for his help with the preparation of the manuscript.

Submitted: 18 January 2001
Revised: 18 May 2001
Accepted: 7 June 2001


  References
Top
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgements
References

  1. Albelda, S.M., Smith, C.W., and Ward, P.A. 1994. Adhesion molecules and inflammatory injury. FASEB J. 8:504-512[Abstract/Free Full Text].

  2. Johnston, B., and Kubes, P. 1999. The {alpha}4-integrin: an alternative pathway for neutrophil recruitment? Immunol. Today. 20:545-550[Medline].

  3. Gao, J.X., and Issekutz, A.C. 1996. Mac-1 (CD11b/CD18) is the predominant ß2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers. Immunology. 88:463-470[Medline].

  4. Ibbotson, G.C., Doig, C., Kaur, J., Gill, V., Ostrovsky, L., Fairhead, T., and Kubes, P. 2001. Functional {alpha}4-integrin: a newly identified pathway of neutrophil recruitment in critically ill septic patients. Nat. Med. 7:465-470[Medline].

  5. Rutter, J., James, T.J., Howat, D., Shock, A., Andrew, D., De Baetselier, P., Blackford, J., Wilkinson, J.M., Higgs, G., and Hughes, B. et al. 1994. The in vivo and in vitro effects of antibodies against rabbit ß2-integrins. J. Immunol. 153:3724-3733[Abstract/Free Full Text].

  6. Issekutz, A.C., and Issekutz, T.B. 1992. The contribution of LFA-1 (CD11a/CD18) and MAC-1 (CD11b/CD18) to the in vivo migration of polymorphonuclear leukocytes to inflammatory reactions in the rat. Immunology. 76:655-661[Medline].

  7. Ley, K., and Tedder, T.F. 1995. Leukocyte interactions with vascular endothelium. New insights into selectin-mediated attachment and rolling. J. Immunol 155:525-528[Abstract].

  8. Plow, E.F., and Zhang, L. 1997. A MAC-1 attack: integrin functions directly challenged in knockout mice. J. Clin. Invest. 99:1145-1146[Free Full Text].

  9. Rosen, H., and Gordon, S. 1987. Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo. J. Exp. Med. 166:1685-1701[Abstract].

  10. Lu, H., Smith, C.W., Perrard, J., Bullard, D., Tang, L., Shappell, S.B., Entman, M.L., Beaudet, A.L., and Ballantyne, C.M. 1997. LFA-1 is sufficient in mediating neutrophil emigration in Mac-1-deficient mice. J. Clin. Invest. 99:1340-1350[Abstract/Free Full Text].

  11. Schmits, R., Kundig, T.M., Baker, D.M., Shumaker, G., Simard, J.J., Duncan, G., Wakeham, A., Shahinian, A., van der Heiden, A., and Bachmann, M.F. et al. 1996. LFA-1–deficient mice show normal CTL responses to virus but fail to reject immunogenic tumor. J. Exp. Med. 183:1415-1426[Abstract].

  12. Andrew, D.P., Spellberg, J.P., Takimoto, H., Schmits, R., Mak, T.W., and Zukowski, M.M. 1998. Transendothelial migration and trafficking of leukocytes in LFA-1-deficient mice. Eur. J. Immunol. 28:1959-1969[Medline].

  13. Coxon, A., Rieu, P., Barkalow, F.J., Askari, S., Sharpe, A.H., von Andrian, U.H., Arnaout, M.A., and Mayadas, T.N. 1996. A novel role for the ß2 integrin CD11b/CD18 in neutrophil apoptosis: a homeostatic mechanism in inflammation. Immunity. 5:653-666[Medline].

  14. Ding, Z.M., Babensee, J.E., Simon, S.I., Lu, H., Perrard, J.L., Bullard, D.C., Dai, X.Y., Bromley, S.K., Dustin, M.L., and Entman, M.L. et al. 1999. Relative contribution of LFA-1 and Mac-1 to neutrophil adhesion and migration. J. Immunol. 163:5029-5038[Abstract/Free Full Text].

  15. Mizgerd, J.P., Kubo, H., Kutkoski, G.J., Bhagwan, S.D., Scharffetter-Kochanek, K., Beaudet, A.L., and Doerschuk, C.M. 1997. Neutrophil emigration in the skin, lungs, and peritoneum: different requirements for CD11/CD18 revealed by CD18-deficient mice. J. Exp. Med. 186:1357-1364[Abstract/Free Full Text].

  16. Berlin-Rufenach, C., Otto, F., Mathies, M., Westermann, J., Owen, M.J., Hamann, A., and Hogg, N. 1999. Lymphocyte migration in lymphocyte function-associated antigen (LFA)-1–deficient mice. J. Exp. Med. 189:1467-1478[Abstract/Free Full Text].

  17. Lim, L.H., Solito, E., Russo-Marie, F., Flower, R.J., and Perretti, M. 1998. Promoting detachment of neutrophils adherent to murine postcapillary venules to control inflammation: effect of lipocortin 1. Proc. Natl. Acad. Sci. USA. 95:14535-14539[Abstract/Free Full Text].

  18. Lund-Johansen, F., and Terstappen, L.W. 1993. Differential surface expression of cell adhesion molecules during granulocyte maturation. J. Leukoc. Biol. 54:47-55[Abstract].

  19. Steeber, D.A., Campbell, M.A., Basit, A., Ley, K., and Tedder, T.F. 1998. Optimal selectin-mediated rolling of leukocytes during inflammation in vivo requires intercellular adhesion molecule-1 expression. Proc. Natl. Acad. Sci. USA. 95:7562-7567[Abstract/Free Full Text].

  20. Sigal, A., Bleijs, D.A., Grabovsky, V., van Vliet, S.J., Dwir, O., Figdor, C.G., van Kooyk, Y., and Alon, R. 2000. The LFA-1 integrin supports rolling adhesions on ICAM-1 under physiological shear flow in a permissive cellular environment. J. Immunol. 165:442-452[Abstract/Free Full Text].

  21. Knorr, R., and Dustin, M.L. 1997. The lymphocyte function-associated antigen 1 I domain is a transient binding module for intercellular adhesion molecule (ICAM)-1 and ICAM-3 in hydrodynamic flow. J. Exp. Med. 186:719-730[Abstract/Free Full Text].

  22. Berlin, C., Bargatze, R.F., Campbell, J.J., von Andrian, U.H., Szabo, M.C., Hasslen, S.R., Nelson, R.D., Berg, E.L., Erlandsen, S.L., and Butcher, E.C. 1995. {alpha}4 integrins mediate lymphocyte attachment and rolling under physiologic flow. Cell. 80:413-422[Medline].

  23. Sriramarao, P., von Andrian, U.H., Butcher, E.C., Bourdon, M.A., and Broide, D.H. 1994. L-selectin and very late antigen-4 integrin promote eosinophil rolling at physiological shear rates in vivo. J. Immunol. 153:4238-4246[Abstract/Free Full Text].

  24. Mazo, I.B., Gutierrez-Ramos, J.C., Frenette, P.S., Hynes, R.O., Wagner, D.D., and von Andrian, U.H. 1998. Hematopoietic progenitor cell rolling in bone marrow microvessels: parallel contributions by endothelial selectins and vascular cell adhesion molecule 1. J. Exp. Med. 188:465-474[Abstract/Free Full Text].

  25. Grabovsky, V., Feigelson, S., Chen, C., Bleijs, D.A., Peled, A., Cinamon, G., Baleux, F., Arenzana-Seisdedos, F., Lapidot, T., and van Kooyk, Y. et al. 2000. Subsecond induction of {alpha}4 integrin clustering by immobilized chemokines stimulates leukocyte tethering and rolling on endothelial vascular cell adhesion molecule 1 under flow conditions. J. Exp. Med. 192:495-506[Abstract/Free Full Text].

  26. Reinhardt, P.H., Elliott, J.F., and Kubes, P. 1997. Neutrophils can adhere via {alpha}4ß1-integrin under flow conditions. Blood. 89:3837-3846[Abstract/Free Full Text].

  27. Neelamegham, S., Taylor, A.D., Burns, A.R., Smith, C.W., and Simon, S.I. 1998. Hydrodynamic shear shows distinct roles for LFA-1 and Mac-1 in neutrophil adhesion to intercellular adhesion molecule-1. Blood. 92:1626-1638[Abstract/Free Full Text].

  28. Hentzen, E.R., Neelamegham, S., Kansas, G.S., Benanti, J.A., McIntire, L.V., Smith, C.W., and Simon, S.I. 2000. Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1. Blood. 95:911-920[Abstract/Free Full Text].

  29. Von Andrian, U.H., Hansell, P., Chambers, J.D., Berger, E.M., Torres Filho, I., Butcher, E.C., and Arfors, K.E. 1992. L-selectin function is required for ß2-integrin-mediated neutrophil adhesion at physiological shear rates in vivo. Am. J. Physiol. 263:H1034-H1044[Abstract/Free Full Text].

  30. Kunkel, E.J., Dunne, J.L., and Ley, K. 2000. Leukocyte arrest during cytokine-dependent inflammation in vivo. J. Immunol. 164:3301-3308[Abstract/Free Full Text].