By
From the * Division of Hematologic Products, Division of Allergenics Products and Parasitology,
and § Facility for Biotechnology Resources, Center for Biologics Evaluation and Research, Food
and Drug Administration, Bethesda, Maryland 20892; and
A.W. Morrow Gastroenterology
and Liver Centre, Royal Prince Alfred Hospital and University of Sydney, Centenary Institute of
Cancer Medicine and Cell Biology, Newtown, New South Wales 2042, Australia
CD26 is a leukocyte activation marker that possesses dipeptidyl peptidase IV activity but whose natural substrates and immunological functions have not been clearly defined. Several chemo-kines, including RANTES (regulated on activation, normal T cell expressed and secreted), have now been shown to be substrates for recombinant soluble human CD26. The truncated RANTES(3-68) lacked the ability of native RANTES(1-68) to increase the cytosolic calcium concentration in human monocytes, but still induced this response in macrophages activated with macrophage colony-stimulating factor. Analysis of chemokine receptor messenger RNAs and patterns of desensitization of chemokine responses showed that the differential activity of the truncated molecule results from an altered receptor specificity. RANTES(3-68) showed a reduced activity, relative to that of RANTES(1-68), with cells expressing the recombinant CCR1 chemokine receptor, but retained the ability to stimulate CCR5 receptors and to inhibit the cytopathic effects of HIV-1. Our results indicate that CD26-mediated processing together with cell activation-induced changes in receptor expression provides an integrated mechanism for differential cell recruitment and for the regulation of target cell specificity of RANTES, and possibly other chemokines.
Monocytes differentiate into macrophages as they migrate from the blood to tissues during immune surveillance. At sites of inflammation, monocyte infiltration
and macrophage accumulation are coordinated, in part, by
chemokines (1). The mechanisms that control the recruitment of monocytes and macrophages by chemoattractants have not been clearly defined, but they may include regulation of the expression of chemokines and their receptors (2)
as well as the modification of chemokine activity by posttranslational processing (3). Several chemokines share a
conserved NH2-X-Pro sequence (where X is any amino
acid) at the NH2 terminus (6), which conforms to the substrate specificity of dipeptidyl exopeptidase IV (DPPIV;
reference 7).1 DPPIV cleaves the first two amino acids from
peptides with penultimate proline or alanine residues, although no natural substrate with immune function has been
identified. This enzyme is also a leukocyte differentiation
antigen, known as CD26 (8), that is expressed on the
cell surface mostly by T lymphocytes and macrophages.
Expression of CD26 has been associated with T cell activation (8) and with susceptibility of a T cell line to infection with macrophage-tropic HIV-1 (11).
In this study, we identify the chemokines RANTES
(regulated on activation, normal T cell expressed and secreted), interferon- Cell Cultures and Transfections.
Monocytes were isolated from
human PBMCs of healthy donors by counter-current centrifugal
elutriation. Monocyte-derived macrophages were prepared by
culturing monocytes for 6 d at a density of 106 cells/ml in serum-free macrophage medium (GIBCO BRL, Gaithersburg, MD)
supplemented with recombinant human (rh) M-CSF (10 ng/ml; R&D Systems, Inc., Minneapolis, MN).
-inducible protein monocyte chemotactic protein (MCP)-2, eotaxin, and IP-10 as the first natural
CD26 substrates with immune function. It is shown that the
cleavage product of RANTES is a chemokine agonist with altered receptor specificity. We also describe, for the first time,
differential changes in the expression pattern of chemokine receptors after activation of monocytes by M-CSF. Therefore, target cell recruitment into inflammatory sites may depend both on the extent of CD26 activity on chemokines and
on the maturational status of the responding cells.
sCD26 Cleavage and Electrospray Mass Spectrometry.
To create
the recombinant soluble (s) human CD26 construct, a signal peptidase cleavage consensus sequence was introduced in the pTZ-CD26.11 cDNA (13) by a Leu to Ala substitution at residue 28. To obtain enzyme negative construct, the Ser at residue 630 was
further replaced by Ala. The two constructs were cloned into the
pEE14.HCMV expression vector and transfected into CHO-K1 cells (14). The enzymatically active (E+) and enzymatically deficient (E) sCD26 proteins were purified from cell culture supernatants of stable transfectants, and were tested in Western blotting
and DPPIV enzyme assays (15). Both proteins had a relative molecular weight of 110 kD and bound equally well to several CD26
mAbs, but only the E+ sCD26 showed detectable DPPIV activity.
Peptide Synthesis. Full-length and truncated RANTES were synthesized with an Applied Biosystems, Inc. (Foster City, CA) peptide synthesizer according to fluorenyl methoxycarbonyl (FMOC) chemistry. FMOC-protected amino acids were added stepwise with ninhydrin monitoring at each cycle. The peptides were folded by air oxidation and purified by reversed-phase- HPLC. Peptide sequences were confirmed by amino acid analysis and Edman sequence analysis, and the molecular masses were confirmed by ES-MS analysis. There was no substantial difference in the activities of chemically synthesized full-length RANTES and rhRANTES(1-68) as judged by the Ca2+ influx and anti- HIV-1 assays used in this study.
Colorimetric DPPIV Enzyme Assay. The p-nitroanilide (pNA)- conjugated Gly-Pro dipeptide substrate and test competitors were mixed and added to human placental DPPIV (Enzyme Systems Products, Dublin, CA), and the resulting mixture was incubated at room temperature in a final volume of 150 µl containing 50 mM tris-HCl (pH 8.0) and 0.15 M NaCl. The final concentrations of DPPIV and Gly-Pro-pNA were 1.25 mU/ml and 400 µM, respectively. The kinetics of the enzyme reaction were monitored by measuring absorbance at 405 nm with a Vmax kinetic microplate reader (Molecular Devices Corp., Menlo Park, CA). The percentage of inhibition of enzyme activity was calculated from the maximal velocity for each sample and from that apparent in the absence of competitor (100% activity).
Reverse Transcriptase-PCR Analysis. Isolated total cellular RNA of monocytes was subjected to first-strand cDNA synthesis. PCR amplification of cDNA was performed for 30 cycles (92°C for 1 min, 40°C for 1 min, 72°C for 1 min) with primers specific for CCR1, CCR2b, CCR3, CCR5, CXCR4, and glyceraldehyde phosphate dehydrogenase (GAPDH). Separated products were stained with SYBR Green I (Molecular Probes, Eugene, OR).
Cytosolic Calcium Measurements.
Cells (107/ml) were washed
and incubated in the dark at 37°C for 45 min in Ca2+ buffer (136 mM NaCl, 4.8 mM KCl, 5 mM glucose, 1 mM CaCl2, 20 mM
Hepes, pH 7.4) supplemented with 5 µM Fura-2 acetoxymethyl ester that had been premixed with 10% Pluronic® F-127 (Molecular Probes). The cells were then washed and resuspended at 2 × 106 cells/ml in Ca2+ buffer containing BSA (1 mg/ml), and portions (2 ml) of the cell suspension were exposed at different time
points in a stirred cuvette at 37°C to chemokines. Fluorescence
was monitored with a scan (Photon Technology Intl., Monmouth Junction, NJ), and data were recorded as the relative ratio
of fluorescence at excitation wavelengths of 340 and 380 nm,
with emission measured at 510 nm. After each measurement,
maximal and minimal fluorescence were assessed by addition of
20 µM ionomycin followed by 5 mM MnCl2.
Assay for HIV-1-induced Cytopathicity.
HOS-CD4.CCR5 cells
(2 × 104) were incubated for 1 h at 37°C with RANTES variants
in 150 µl of culture medium containing 20% FCS, and were then
mixed with 50 µl (2 × 105 cells/ml) of uninfected PM1 cells or
PM1 cells chronically infected with MV3-HXB2 virus. After 3 d,
photomicrographs of cultures were taken and cell viability was
measured by adding of 50 µl of 1 mg/ml 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide solution
containing 20 µM phenazine methosulfate and recording the OD
at 450 nm. Data are expressed as the percentage of inhibition of
cytopathicity [calculated as 100% × (R V)/(U
V), where U,
V, and R represent OD values obtained for HOS-CD4.CCR5
cells cultured with uninfected PM1 cells, or with HIV-1-infected
cells in the absence or presence of chemokine, respectively].
ES-MS analysis revealed that 100 nM rhRANTES
underwent partial to complete hydrolysis when incubated
overnight at 37°C with increasing amounts (25-250 µU)
of sCD26 (Fig. 1). Taking into account cationization (K+)
of the multiply charged ions, the measured molecular
masses of the native and degraded polypeptides corresponded to the theoretical masses of full-length (residues 1-68)
and truncated (residues 3-68) forms of RANTES, respectively. The calculated difference between the molecular
masses of the native and the truncated forms ranged from
183 to 185 daltons, which is consistent with the expected mass (184 daltons) of a released Ser-Pro dipeptide, the predicted NH2 terminus of RANTES (16). In contrast to the
effect of enzymatically active sCD26, shortened RANTES was
not generated by incubation of the chemokine with a mutant sCD26 deficient in enzyme activity (Fig. 1). RANTES
also inhibited, possibly in a competitive manner, the rapid
hydrolysis of a pNA-conjugated Gly-Pro dipeptide by human placental DPPIV, as measured in a colorimetric enzyme assay (Fig. 2). The efficacy of inhibition by chemically
synthesized RANTES(1-68) was similar to that observed
with the DPPIV substrate and competitive inhibitor Ile-Pro-Ile (Diprotin A; reference 17), whereas RANTES(3-68)
did not inhibit the reaction.
Sensitivity to CD26-mediated cleavage was not a unique property of RANTES (Table 1). Cleavage products with the predicted molecular masses were also evident in samples of MCP-2, eotaxin, and IP-10 after incubation with sCD26. In contrast, MCP-1, which has a 62% sequence similarity with MCP-2 including the NH2-terminal Gln-Pro dipeptides, was not cleaved by the enzyme under the same experimental conditions.
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To investigate the functional significance
of DPPIV-mediated truncation of RANTES, we compared the effects of chemically synthesized RANTES(1-68)
and RANTES(3-68) on monocytes and monocyte-derived macrophages. Both resting cells and cells activated with
M-CSF were analyzed because reverse transcriptase-PCR
revealed marked changes in the abundance of chemokine
receptor transcripts in response to M-CSF activation (Fig.
3). In resting cells, transcripts encoding the chemokine receptors CCR1, CCR2b, or CXCR4, as well as control
GAPDH mRNA, were readily detectable, whereas CCR5
receptor transcripts were virtually absent. After differentiation to macrophages, the intensity of the CXCR4 and
GAPDH signals remained virtually unchanged, whereas the
abundance of CCR1 and CCR5 mRNAs increased substantially and the CCR2b transcript virtually disappeared. CCR3 mRNA was not detected in either cell type.
Transient changes in the cytosolic free Ca2+ concentration ([Ca2+]i) were recorded after stimulation of monocytes
or macrophages with an optimal concentration of RANTES-
(1-68) or RANTES(3-68), and the effects were compared
with those of other chemokines (Fig. 4). Addition of 100 nM RANTES(1-68) to cells loaded with the fluorescent Ca2+ probe Fura-2 induced a rapid increase in [Ca2+]i in
both monocytes and macrophages. In contrast, the same
concentration of RANTES(3-68) increased [Ca2+]i in macrophages but not in monocytes. Among the other chemo-kines tested, macrophage inflammatory protein (MIP)-1,
MCP-1, MCP-3 (1, 6), and stromal-derived factor-1
(SDF-1
; references 18) also increased [Ca2+]i in resting
monocytes, whereas MCP-2 (21) induced a barely detectable response and MIP-1
(1, 6) was inactive. On the basis
of the previously described receptor specificities of these chemokines (1, 6, 19, 20), the obtained activity pattern is
consistent with expression of CCR1, CCR2b, and
CXCR4 receptors on monocytes (Fig. 3). Macrophages
showed marked Ca2+ responses to MIP-1
, MIP-1
,
MCP-2, MCP-3, and SDF-1
, but were resistant to
MCP-1, consistent with the presence of transcripts encoding CCR1, CCR5, and CXCR4, and the absence of those
encoding CCR2b, in these cells (Fig. 3).
RANTES(3-68) Is a Chemokine Agonist, with Altered Receptor Specificity.
Agonists that act at common chemokine
receptors block each other's activity as a result of receptor
desensitization, whereas responses to chemokines that act at
different receptors generally are not affected (1, 6). Therefore, we performed comparative desensitization experiments
to define the types of receptors that mediate the effects of
native versus truncated RANTES in macrophages (Fig. 5).
Macrophages that were stimulated first with 100 nM
RANTES(1-68) did not exhibit a second Ca2+ response
when challenged with the same dose of either full-length or truncated RANTES. In contrast, cells stimulated with
100 nM RANTES(3-68) fully retained their ability to respond to a subsequent challenge with full-length RANTES,
but were desensitized to the effect of the truncated form.
These results suggest that the receptor repertoire available for truncated RANTES is more restricted than that
available for the native chemokine. To further characterize the receptor usage of the different forms of RANTES
and other chemokines, we also studied the sensitivity of
MIP-1-, MCP-3-, and SDF-1
-induced Ca2+ responses
to RANTES-mediated receptor desensitization (Fig. 5). Of
the known receptors, RANTES signals via CCR1, CCR4,
and CCR5, whereas MIP-1
acts at CCR5 exclusively and
MCP-3 binds only to CCR1 and CCR2b at the concentrations used in our experiments (1, 6). The only receptor
known to bind SDF-1
is CXCR4 (19, 20). Pretreatment
of macrophages with full-length RANTES blocked the
ability of MIP-1
and MCP-3, but not that of SDF-1
, to
increase [Ca2+]i. In contrast, RANTES(3-68) desensitized
cells to the effect of MIP-1
but did not affect the response
to MCP-3 or SDF-1
. These results are consistent with previous data on RANTES-induced receptor desensitization
(1) and with our data on chemokine receptor mRNA abundance (Fig. 3). They suggest that, in M-CSF-activated macrophages, full-length RANTES shares CCR1 and CCR5
receptors with MCP-3 and MIP-1
, respectively. Our results also indicate that, without its two NH2-terminal residues, RANTES is still able to signal via CCR5 but can no
longer act at the CCR1 receptor.
Removal of the NH2-Ser-Pro Residues Affects the CCR1-, but not the CCR5-mediated Signaling of RANTES.
HEK-293 cells
expressing CCR5 and HOS-CD4 cells expressing CCR1
were loaded with Fura-2 and exposed to various concentrations of RANTES(1-68) or RANTES(3-68). The two
RANTES variants showed similar abilities to increase [Ca2+]i
in the CCR5 transfectant (Fig. 6 A); the responses were
dose dependent, with 10 nM of each variant sufficient to
induce a maximal Ca2+ response. In contrast, in the cells
expressing CCR1, the amount of RANTES(3-68) required
to produce a detectable Ca2+ response was ~100 times that
for RANTES(1-68) (Fig. 6 B); the effect of RANTES(1-68)
saturated at 50 nM, whereas that of RANTES(3-68) appeared not to have achieved saturation at 200 nM. Furthermore, bidirectional cross-desensitization between the two
RANTES variants was evident only with the cells expressing CCR5 (Fig. 6 C); in the CCR1 transfectant, cross-desensitization was induced by full-length RANTES but
not by the truncated form, which also did not exhibit self-desensitization (Fig. 6 D). Control cells transfected with vector alone or with vectors encoding CCR2b, CCR3, or
CXCR4 did not respond to these ligands (data not shown).
These results thus confirm that the native and CD26-truncated RANTES variants exhibit markedly different activities at the CCR1 receptor.
RANTES(3-68) Is a Potent Inhibitor of HIV-1.
In addition to their function in chemotaxis, RANTES, MIP-1,
and MIP-1
each inhibit HIV-1 infection by competitive
binding to CCR5 (22), and this inhibition does not require receptor-mediated cell signaling (27, 28). To examine whether removal of the two NH2-terminal residues
affects the antiviral activity of RANTES, we mixed HOS-CD4 cells expressing recombinant CCR5 and PM1 cells chronically infected with the macrophage-tropic recombinant MV3-HXB2 virus and cocultured them in the absence
or presence of various concentrations of RANTES(1-68) or
RANTES(3-68). Both RANTES variants inhibited HIV-1-induced syncytium formation and cytopathicity (Fig. 7).
Thus, similar to signaling activity through CCR5, competitive inhibition of HIV-1 infection does not require the
NH2-terminal Ser-Pro residues of RANTES.
Chemical modifications at the NH2 terminus of chemo-kines have been previously suggested to produce polypeptides that are antagonists of the native chemoattractants (3, 4). However, these alterations did not correspond to the physiological specificities of known enzymes and no natural equivalents of the modified chemokines were identified. In contrast, the CD26 cleavage product of RANTES, RANTES (3-68), acts as a chemokine agonist with altered receptor specificity. Hydrolysis by CD26 might explain why RANTES (3-68) has been isolated as a second component in addition to intact RANTES from culture supernatants of stimulated human fibroblasts, skin samples, and platelet preparations (29, 30). The CC-chemokines RANTES, MCP-2, and eotaxin, and the CXC-chemokine IP-10 are the first immune modulators and the longest polypeptides identified as natural substrates for CD26.
CD26 exists in both soluble and membrane-expressed forms. Secreted forms of CD26 have been identified in cell cultures and in human serum (31, 32), although CD26 may be more active when expressed as an ectoenzyme at high concentrations on endothelial cells, hepatocytes, kidney brush border membranes, and leukocytes (10). Upregulation of CD26 expression on T lymphocytes and macrophages has been linked to cell activation and development of immunological memory (10). Thus, activation-induced changes in CD26 expression could affect the course of an inflammatory response by modifying the target cell specificity of RANTES or other chemokines, and by regulating the equilibrium between the migrating cell subsets. We are currently addressing whether cells with different levels of CD26 expression (e.g., naive versus memory T cells) secrete truncated forms of RANTES or other chemoattrac-tants, or are capable of modifying exogenous chemokines.
The differential effects of CD26-truncated RANTES on
monocytes versus macrophages illustrate a role for cell differentiation in regulating chemokine sensitivity through altered receptor expression. Our functional and receptor
transcript data indicate that CCR1 and CCR2b may be the
two principal CC chemokine receptors in resting monocytes, although other unidentified and functionally overlapping receptors may also contribute to chemokine function. Cell differentiation markedly changes the pattern of
chemokine sensitivity by reducing CCR2b expression,
thereby rendering the cells resistant to MCP-1, while increasing CCR5 expression, thereby augmenting the responses to CD26-truncated RANTES and MIP-1. An increase in CCR5 expression may also render macrophages
more susceptible to infection by macrophage-tropic variants of HIV-1. We have shown that macrophages also express CXCR4, the coreceptor for T cell line-tropic HIV-1
variants (33, 34), as assessed by receptor transcript abundance and functional activity of the CXCR4 ligand SDF-1
. Nevertheless, activated macrophages are relatively resistant to infection by T cell line-tropic HIV-1 variants
(35), which suggests that factors other than CXCR4 may
also be required for efficient infection of macrophages by these types of viruses.
Removal of two NH2-terminal residues by CD26 abolishes the interaction of RANTES with CCR1, but does not affect the anti-HIV-1 activity or the CCR5 signaling properties of the chemokine. Proline residues also influence the susceptibility of proximal peptide bonds to proteolytic enzymes (6), and so the removal of such residues by CD26 may also reduce the half-life of RANTES and other chemo-kines during an inflammatory response. It will be important to determine whether CD26-mediated cleavage is a general mechanism for changing the receptor specificity and functional activity of other chemokines, including those examined in this study (MCP-2, eotaxin, and IP-10).
Many, but not all CC- and CXC-chemokines contain the X-Pro- or X-Ala-NH2-terminal sequence and are potential substrates of DPPIV. We are currently exploring whether the inability of CD26 to cleave MCP-1 is due to aggregation of this chemokine under these experimental conditions or to a conformational requirement of the enzyme that is not fulfilled by MCP-1. Selectivity of CD26 activity on chemokines may function to reduce redundancy in chemokine target cell specificity as illustrated by the different activity of full-length and truncated RANTES on monocytes versus macrophages. Finally, truncated analogs of chemokines with selective activity on distinct functional receptors, or analogs that resist CD26 cleavage, may prove therapeutically beneficial in blocking or inducing the infiltration of specific subsets of effector cells mediating inflammation, allergy, and antitumor responses.
Address correspondence to Michael A. Norcross or Tamas Oravecz, Division of Hematologic Products, Center for Biologics Evaluation and Research, FDA, NIH, Bldg 29B, Rm 4E12, HFM-541, Bethesda, MD 20892. Phone: 301-827-0793; FAX: 301-480-3256; E-mail: miken{at}helix.nih.gov or tamas{at}helix.nih.gov
Received for publication 4 June 1997 and in revised form 22 September 1997.
Note added in proof. We note that a CD8+ T cell-derived HIV-1 suppressor activity has been recently identified as a truncated form of macrophage-derived chemokine (MDC), missing a glycine-proline dipeptide from the NH2 terminus (Pal, R., A. Garzino-Demo, P.D. Markham, J. Burns, M. Brown, R.C. Gallo, and A.L. DeVico. 1997. Science. 278:695-698). Based on our results, we suggest that truncation of MDC is a consequence of CD26-mediated cleavage that may have resulted in enhanced MDC antiviral activity.We thank K. Faust and V. Calvert for help in separation of mononuclear cells, C. Abbott for mutant CD26 cDNAs, and M. Samson for the CCR5 vector. The HOS-CD4 cell lines were obtained from N. Landau through the AIDS Research and Reference Reagent Program (Division of AIDS, National Institute of Allergy and Infectious Diseases).
This study was supported in part by funds from the National Institutes of Health AIDS Targeted Antiviral Program and from the National Health and Medical Research Council of Australia (M.D. Gorrell).
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