Regulation of Interleukin (IL)-12 Receptor beta 2 Subunit Expression by Endogenous IL-12: A Critical Step in the Differentiation of Pathogenic Autoreactive T Cells

By John T. Chang, Ethan M. Shevach, and Benjamin M. Segal

From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892

    Abstract
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Abstract
Introduction
Materials and Methods
Results
Discussion
References

The interleukin (IL)-12 receptor (R)beta 2 subunit is the critical molecule involved in maintaining IL-12 responsiveness and controlling T helper cell type 1 lineage commitment. We demonstrate that IL-12 and interferon (IFN)-gamma play separate, but complementary, roles in regulating IL-12Rbeta 2 expression on antigen-specific CD4+ T cells. These results are consistent with our previous observation that IL-12 can promote autoimmune disease through IFN-gamma -independent as well as -dependent pathways. Therefore, we compared the induction of IL-12 by, and the expression of the IL-12Rbeta 2 subunit on, myelin basic protein (MBP)-specific T cells from experimental allergic encephalomyelitis (EAE)-susceptible SJL (H-2s) mice and from EAE- resistant B10.S mice (H-2s). B10.S mice had an antigen-specific defect in their capacity to upregulate the IL-12Rbeta 2 subunit. Defective expression was not secondary to the production of suppressive cytokines, but to a failure of B10.S MBP-specific T cells to upregulate CD40 ligand expression and to induce the production of IL-12. IL-12Rbeta 2 expression as well as encephalitogenicity of these cells could be restored by the addition of IL-12. These results suggest that the development of immunotherapies that target the IL-12Rbeta 2 subunit may be useful for the treatment of autoimmune diseases.

Key words: autoimmunity;  experimental allergic encephalomyelitis;  T helper cell type 1 lymphocytes;  interferon gamma ;  CD40 ligand
    Introduction
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

Animal models of organ-specific autoimmune diseases are uniformly mediated by autoreactive Th1 lymphocytes, whether they develop spontaneously or are induced by immunization with autoantigen in CFA. For example, the production of IFN-gamma and/or TNF-alpha has been implicated in the pathogenesis of experimental allergic encephalomyelitis (EAE),1 myasthenia gravis, collagen-induced arthritis (CIA), insulin-dependent diabetes mellitus (IDDM) in nonobese diabetic mice, and the spectrum of organ- specific autoimmune disease that follows neonatal thymectomy (1). Th1 cells have also been implicated in the pathogenesis of organ-specific diseases in humans, including multiple sclerosis, IDDM, inflammatory arthritis, and autoimmune thyroiditis (9). Although successful induction of autoimmune diseases in mice genetically deficient in IFN-gamma , TNF-alpha , or lymphotoxin alpha  have cast doubt on the involvement of a single proinflammatory Th1 cytokine in disease pathogenesis (13), and have even demonstrated protective effects of some of these proinflammatory mediators, studies in a number of disease models demonstrate a critical role for IL-12 in the generation of Th1 effectors (17). IFN-gamma -/- mice exhibit enhanced susceptibility to EAE as well as to CIA, whereas IL-12-/- mice are completely resistant to induction of either disease (18). Furthermore, neutralization of IL-12 in IFN-gamma -/- mice protected them from both EAE and CIA. In SJL mice, administration of a polyclonal antiserum to IL-12 was protective during either the induction or effector phase of adoptively transferred EAE (20, 21). Similarly, neutralization of IL-12 in mice with established colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) led to rapid and complete recovery (22); furthermore, anti-IL-12, but not anti-IFN-gamma , was recently found to be therapeutic in another model of autoimmune colitis that arises in IL-10-/- mice (23). Interference with the biological activity of IL-12 may therefore be an appropriate target for the treatment of autoimmune disease in humans.

Collectively, these studies suggest that the successful differentiation and/or function of autoimmune effector cells must be dependent on their ability to respond to quantities of IL-12 that are available in the microenvironment during critical time points. Studies from several groups have demonstrated that the expression of the IL-12 receptor plays a critical role in determining the Th1/Th2 balance during the course of an immune response. The IL-12Rbeta 2 subunit is not expressed on resting T cells, but is induced at low levels after engagement of the TCR by antigen. Maintenance of a level of IL-12Rbeta 2 subunit expression sufficient to allow signaling by IL-12 and therefore Th1 differentiation is critically dependent on the relative amounts of IFN-gamma and IL-4 in the microenvironment during T cell priming (24). IL-4 inhibits IL-12Rbeta 2 subunit expression, but this inhibition can be overcome by IFN-gamma , even in cells that have begun to differentiate along the Th2 pathway. Although the IL-12Rbeta 2 subunit can be induced in the presence of exogenous IL-12 in the absence of IFN-gamma and IL-4, the role of endogenous IL-12 itself in direct induction of IL-12Rbeta 2 expression has not yet been examined. The direct induction of IL-12Rbeta 2 expression may be particularly relevant to the development of autoimmune effectors in IFN-gamma -/- mice.

In this report, we initially used cytokine-deficient mice and neutralizing anti-cytokine mAbs to critically examine the relative roles of IL-12 and IFN-gamma in the induction and maintenance of the IL-12Rbeta 2 subunit on CD4+ T cells that have been primed against a conventional foreign antigen. We demonstrate that in wild-type mice IL-12 and IFN-gamma play separate as well as complementary roles in regulating IL-12Rbeta 2 expression. However, sustained expression of IL-12Rbeta 2 can be induced in IFN-gamma -/- mice by an IL-12-dependent pathway. These findings raised the possibility that the regulation of IL-12Rbeta 2 expression by IL-12 itself may play a role in the generation of pathogenic autoreactive T cells. Therefore, we extended these studies and compared the induction of the IL-12Rbeta 2 subunit on MBP-specific T cells derived from a strain of mice (SJL, H-2s) that is highly susceptible to EAE with T cells from a strain (B10.S, H-2s) that is resistant to EAE. We demonstrate that B10.S mice have an antigen-specific defect in their capacity to upregulate the IL-12Rbeta 2 subunit. This defective expression of the IL-12Rbeta 2 subunit is not secondary to the production of suppressive cytokines or to a decreased functional avidity of the TCR, but to a failure of the B10.S MBP-specific T cells to upregulate CD40 ligand (CD40L) expression, which is an important stimulus for the endogenous production of IL-12 by APCs. Because IL-12Rbeta 2 expression as well as the encephalitogenicity of these cells can be restored by the addition of exogenous IL-12 or IL-12-inducing agents (27, 28), we classify the B10.S MBP-specific cells as a novel population of autoreactive preautoimmune T (Tpre-A) cells that have emerged from the thymus with a defect in their capacity to differentiate into mature Th1 effector cells. However, these autoreactive cells retain the potential to differentiate into pathogenic T cells upon exposure to IL-12 induced by environmental or infectious agents.

    Materials and Methods
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

Mice.

SJL/J and C57BL/6 mice were obtained from the National Cancer Institute. Breeding pairs of C57BL/6 IL-12-/- (N6) and C57BL/6 IFN-gamma -/- were originally provided by J. Magram (Hoffman-La Roche, Nutley, NJ) and D. Dalton and T. Stewart (Genetech Inc., South San Francisco, CA), respectively. B10.S mice were obtained from both McLaughlin Research Institute and Taconic Farms. All mice were housed under specific pathogen-free conditions. They were exclusively female and between 2 and 4 mo of age when experiments were started.

Peptides.

Peptides corresponding to residues 260-283 of Influenza A nucleoprotein (NP260-283, ARSALILRGSVAHKSCLPACVYGP), residues 87-106 of myelin basic protein (MBP87-106, VVHFFKNIVTPRTPPPSQGK), and residues 139-151 of proteolipid protein (PLP139-151, HSLGKWLGHPDKF) were synthesized and purified by HPLC by the Laboratory of Molecular Structure, Peptide Synthesis Laboratory (NIAID, National Institutes of Health [NIH]). Chicken OVA was purchased from Sigma Chemical Co.

Immunization.

Mice were immunized subcutaneously at four sites over the flanks with an emulsion consisting of equal volumes of CFA (DIFCO Labs.) and antigen dissolved in PBS. Doses of antigen used were as follows: MBP87-106, 100 µg; NP260-283, 5 µg; OVA, 100 µg; PLP139-151, 100 µg.

Disease Induction.

For disease induction by adoptive transfer, donor mice were immunized with 100 µg of MBP or PLP in CFA (1:1); 10-14 d later, draining LN cells were cultured for 96 h with MBP or PLP. Recovered cells (6 × 107) were injected intraperitoneally into naive syngeneic recipients that were examined daily for signs of EAE and rated on a five-point scale as previously described (20).

Cell Cultures.

10-14 d after immunization, draining LN cells (axillary and inguinal) were removed and processed as previously described (20). In brief, single cell suspensions of spleen or LN tissue were prepared by passage through wire mesh and red blood cells lysed with ACK buffer (NIH Media Unit). Cells (4 × 106/ml) were cultured in RPMI 1640 containing 10% FCS and standard supplements (25) for 24-72 h in the presence of MBP87-106 (50 µg/ml), NP260-283 (5 µg/ml), PLP (50 µg/ml), or OVA (100 µg/ml). To generate short-term lines, draining LN cells were cultured in MBP or NP for 4 d, washed extensively, and rested for 7 d in complete media. For maintenance of lines, T cells (106/ml) were restimulated with antigen in the presence of irradiated, syngeneic splenocytes (4 × 106/ml) every 10 d. For IL-12 assays, T cells (106/ml) were restimulated with or without antigen in the presence of syngeneic peritoneal exudate macrophages (5 × 105/ml). To generate peritoneal exudate macrophages, B10.S or SJL mice were injected with 3 ml fluid thioglycollate media (NIH Media Unit). 3 d later, macrophages were removed from the peritoneum of treated mice, washed, and used in experiments. In some experiments, cells were purified using T cell enrichment columns (R&D Systems). T and/or NK cell depletion was performed by treating cells with anti-Thy1.2 (clone HO-13.4) culture supernatants or anti-NK1.1 antibody (clone PK136) followed by treatment for 45 min at 37°C with rabbit complement (Cedarlane Labs.).

Proliferation Assays.

LN cells (5 × 105/0.2 ml) were cultured with various concentrations of antigen or with media alone for 4 d in 96-well round-bottomed plates (Costar Corp.). Wells were pulsed for the final 16 h of culture with 1 µCi of [3H]TdR (Amersham) and counted as previously described (20).

Antibodies and Cytokines.

Where specified, cytokines or neutralizing antibodies were added to the primary cultures as follows: IL-12, 20 ng/ml (gift of S. Wolf, Genetics Institute, Cambridge, MA); recombinant murine IFN-gamma , 30 ng/ml (PharMingen); recombinant murine IL-10, 10 ng/ml (PharMingen); anti-mouse IFN-gamma , 10 µg/ml (clone XMG 1.2); rat anti-mouse IL-12, 10 µg/ml (clone C17.8); rat anti-mouse IL-10, 10 µg/ml (clones SXC-1 and SXC-2); rat IgG, 10 µg/ml (Sigma Chemical Co.); and anti-CD3 (2C11), 1.0 µg/ml. In certain studies, mice were injected intraperitoneally on days 0, 3, and 6 with 1 mg of either control rat IgG or rat anti-mouse IL-10 (clones SXC-1 and SXC-2).

Northern Blot Analysis.

Total RNA was isolated from LN cell cultures using RNAzol RNA isolation solvent (Tel-Test). Samples (10 µg of total RNA per lane) were run on a 1.2% agarose gel containing MOPS buffer and formaldehyde, and blotted onto a Hybond-N nylon membrane (Amersham). Membranes were baked for 2 h at 80°C, then probed for murine IL-12Rbeta 2 subunit, murine IL-12Rbeta 1 subunit, or beta -actin. The following primer sets (Bio-Synthesis) were used to generate oligonucleotide cDNA probes: IL-12Rbeta 2 forward CTG CAC CCA CTC ACA TTA AC; IL-12Rbeta 2 reverse CAG TTG GCT TTG CCC TGT GG; IL-12Rbeta 1 forward GAG GAG GCG GCT CTC CTC AG; IL-12Rbeta 1 reverse ACA TTC CTC CTG CTC CAG GG. The beta -actin primer set was purchased from Clontech. PCR was performed for 40 cycles with the following parameters: 94°C, 30 s; 58°C, 30 s; 72°C, 1 min. cDNA probes were run on a 1.2% agarose gel, and purified using the Wizard PCR DNA purification system (Promega). PCR fragments (50 ng) were labeled with [32P]dCTP using an oligolabeling kit (Pharmacia). Blots were prehybridized for 1 h at 42°C, followed by overnight hybridization with labeled probe at 42°C. Blots were then washed for 30 min in 2× SSC, 0.1% SDS buffer (room temperature) followed by 30 min in 0.1× SSC, 0.1% SDS buffer (55°C for IL-12Rbeta 2 and IL-12Rbeta 1; 65°C for beta -actin).

PCR.

cDNA was reverse transcribed from 5 µg of RNA using Superscript II (GIBCO BRL). The following CD40L primers have been previously described: forward CCC TTA AGC TTG CAT GAT AGA AAC ATA corresponding to residues 10-26; and reverse TAG AGC TCG AGG TTC AGA GTT TGA GTA AGC C corresponding to residues 778-786 (29). A competitive PCR MIMIC was constructed from a kit according to the manufacturer's instructions (Clontech). The pMus-3 plasmid (gift of Dr. Nancy Noben-Trauth, NIH) was used in conjunction with the following primers to quantitate beta 2 microglobulin: forward TGA CCG GCT TGT ATG CTA TC; reverse CAG TGT GAG CCA GGA TAT AG. PCR reactions were carried out using Ready To Go PCR beads (Pharmacia) with 1 µl of MIMIC and 2 µl of cDNA reaction. PCR was performed for 40 cycles with the following parameters: 94°C, 30 s; 58°C, 30 s; 72°C, 1 min. PCR products were separated on a 1.2% agarose gel, and densitometry performed on an Eagle Eye Gel Reader (Stratagene). A relative ratio of CD40L/beta 2 microglobulin was calculated, and data were expressed as the percentage change of stimulated levels of CD40L/beta 2 microglobulin compared with background levels.

Cytokine ELISA.

IFN-gamma was quantified using a sandwich ELISA technique based on noncompeting pairs of antibodies as previously described (28). IL-12p40 was quantified using the mAbs produced by clones C17.5 and C15.6 (gifts of G. Trinchieri, Wistar Institute, Philadelphia, PA) for capture and detection, respectively.

    Results
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
Independent Effects of IL-12 and IFN-gamma on the Expression of the IL-12R.

Previous studies have focused either on the role of IFN-gamma in the upregulation or the role of IL-4 in the downregulation of IL-12Rbeta 2 expression (25, 30). However, as IL-12 is a potent inducer of IFN-gamma production by T and NK cells, it was impossible to determine from these experiments whether IL-12 directly, or indirectly via IFN-gamma , induced the expression of IL-12R. To distinguish between these possibilities, we immunized C57BL/6 wild-type, IL-12-/-, and IFN-gamma -/- mice with OVA in CFA. 10 d later, draining LN cells were cultured in vitro for 3 d. Total RNA was extracted and used to perform Northern blot analysis. LN cells from C57BL/6 wild-type mice upregulated IL-12Rbeta 2 subunit mRNA strongly in response to stimulation with OVA in vitro (Fig. 1 A); the level of IL-12Rbeta 2 mRNA was diminished by the addition of anti-IL-12 or anti-IFN-gamma to the cultures. OVA-reactive LN cells from IFN-gamma -/- mice also upregulated IL-12Rbeta 2 subunit mRNA to a level comparable with that induced on wild-type LN cells (Fig. 1 B). The addition of anti-IL-12 to cultures of OVA-primed LN cells from IFN-gamma -/- mice markedly reduced their expression of IL-12Rbeta 2; conversely, the addition of IL-12 to these cultures markedly enhanced the level of expression of IL-12Rbeta 2 subunit above that seen when the cells were stimulated with OVA alone. In contrast to the results with cells from IFN-gamma -/- mice, only weak upregulation of IL-12Rbeta 2 subunit expression in response to stimulation with OVA was seen with LN cells from IL-12-/- mice; IL-12Rbeta 2 expression was moderately enhanced by the addition of exogenous IFN-gamma to the cultures (Fig. 1 C). Taken together, these studies demonstrate that a low level of IL-12Rbeta 2 expression is induced by TCR signaling alone and that IL-12 and IFN-gamma have independent as well as complementary effects in promoting/maintaining the expression of the IL-12Rbeta 2 subunit.


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Fig. 1.   IL-12 and IFN-gamma have independent as well as complementary effects on the maintenance of IL-12Rbeta 2 subunit expression. C57BL/6 wild-type, IFN-gamma -/-, and IL-12-/- were immunized with OVA; 10 d later, LN cells were removed and cultured for 3 d. (A) C57BL/6 wild-type LN cells were stimulated in the presence of OVA with or without anti-IL-12 (10 µg/ml) or anti-IFN-gamma (10 µg/ml). (B) IFN-gamma -/- LN cells were stimulated in the presence of OVA with or without anti-IL-12 (10 µg/ml) or IL-12 (20 ng/ml). (C) IL-12-/- LN cells were stimulated in the presence of OVA with or without IFN-gamma (30 ng/ ml). Results shown are representative of four experiments each using LN cells pooled from five or more mice.

B10.S MBP-reactive T Cells Fail to Induce IL-12p40 Production by Peritoneal Exudate Macrophages and Manifest a Deficiency in CD40L Expression.

We have previously shown that B10.S, but not SJL mice, are resistant to the induction of EAE and that the major difference between these strains was that T cells from B10.S, but not SJL mice, demonstrated an antigen-specific defect in IFN-gamma production upon activation in vitro (28). Since our data clearly demonstrated that endogenous IL-12 is required for optimal IL-12Rbeta 2 subunit expression, we questioned if the defect in the ability of B10.S MBP-reactive T cells to produce IFN-gamma was secondary to a failure of APCs to produce IL-12. It is unlikely that an intrinsic defect in IL-12 production is present in B10.S APCs, as it would be manifested as impaired IFN-gamma production in response to multiple antigens, whereas our results indicate marked antigen-specificity. Furthermore, purified dendritic cells and splenocytes from B10.S and SJL mice produced comparable amounts of IL-12p70 heterodimer when stimulated with either anti-CD40 or LPS/IFN-gamma (data not shown). Therefore, it seemed more likely that any deficiency in IL-12 production must result from a defect of the T cell to prime the APCs to produce IL-12. We generated B10.S MBP- and NP-reactive T cell lines and stimulated them with antigen in the presence of syngeneic peritoneal exudate macrophages. After 48 h of stimulation, B10.S NP-reactive T cell lines induced high levels of IL-12p40 production by macrophages, whereas B10.S MBP-reactive T cell lines failed to induce any detectable IL-12p40 production (Fig. 2 A). In contrast, SJL MBP- and NP-reactive T cell lines induced comparable amounts of IL-12p40 production from macrophages (Fig. 2 B).


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Fig. 2.   B10.S MBP-reactive, but not NP-reactive, T cell lines fail to induce IL-12p40 production by peritoneal exudate macrophages. Short-term T cell lines (106/ml) from B10.S (A) or SJL mice (B) were restimulated with syngeneic peritoneal exudate macrophages (5 × 105/ml) with or without antigen and IL-12p40 production was measured at 48 h. Background levels of IL-12p40 were subtracted. Results shown are representative of two experiments.

Because the interaction of CD40L on T cells and CD40 on APCs is a major stimulus for IL-12 production, these results raised the possibility that the failure of activated B10.S MBP-reactive T cells to express sufficient levels of CD40L upon activation is responsible for their failure to prime APCs to produce IL-12. We used a competitive PCR mimic to quantitate the relative levels of CD40L mRNA expressed upon antigenic activation by B10.S MBP- and NP-reactive LN cells. MBP-reactive LN cells failed to upregulate CD40L mRNA, whereas NP-reactive LN cells upregulated CD40L mRNA eightfold over unstimulated background levels (Fig. 3). Thus, the relative deficiency in CD40L expression by B10.S MBP-reactive LN cells may account for their failure to induce IL-12 production by APCs.


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Fig. 3.   B10.S MBP-reactive, but not NP-reactive, LN cells fail to upregulate CD40L upon activation. B10.S mice were immunized with a combination of MBP and NP. On day 12, draining LN cells were removed and cultured with antigen for 3 d. Total RNA was extracted, and competitive PCR was performed for CD40L and beta 2 microglobulin. Data expressed as the percentage change of stimulated levels of CD40L/beta 2 microglobulin compared with background levels. Results are representative of three experiments each using LN cells pooled from five or more mice.

MBP-specific T Cells from EAE-resistant B10.S Mice Fail to Upregulate IL-12Rbeta 2 Subunit mRNA.

To test directly whether the failure of the B10.S MBP-specific T cells to induce IL-12 leads to a deficiency in the expression of adequate levels of IL-12Rbeta 2 subunit, draining LN cells from SJL and B10.S mice that had been primed 12 d previously with a combination of MBP and NP in CFA were cultured in vitro for 72 h with either MBP or NP and evaluated for IL-12R expression. LN cells from SJL mice upregulated IL-12Rbeta 2 subunit mRNA strongly in response to stimulation with either MBP or NP (Fig. 4 A, left). In contrast, LN cells from B10.S mice upregulated IL-12Rbeta 2 subunit mRNA only in response to stimulation with NP, but not MBP (Fig. 4 A, right). Since NK cells and dendritic cells have been reported to express IL-12R (26, 31), T cells were purified from LN cells from immunized B10.S mice and depleted of NK cells. These T cells were combined with T cell-depleted, NK cell-depleted splenocytes from naive B10.S mice and stimulated for 72 h, then evaluated for IL-12Rbeta 2 subunit expression. Results similar to those shown in Fig. 4 A were obtained, thus excluding dendritic cells or NK cells as the source of IL-12R observed (Fig. 4 B). In contrast to the IL-12Rbeta 2 subunit, which was induced only upon antigen stimulation, IL-12Rbeta 1 subunit was constitutively expressed with no consistent patterns of antigen-induced upregulation (Fig. 4 A), in agreement with previously published reports (25, 26).


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Fig. 4.   The ability of LN cells to express IL-12Rbeta 2 subunit mRNA correlates with susceptibility to EAE. (A) B10.S and SJL were immunized with MBP/NP. On day 12, draining LN cells were removed and cultured with antigen or anti-CD3 for 3 d. Total RNA was extracted, and Northern blot analysis was performed for IL-12Rbeta 2 and beta 1 subunits, and beta -actin. Results shown are representative of five experiments each using LN cells pooled from five or more mice. (B) T cells were purified from LN cells from B10.S mice coimmunized with MBP/NP and subsequently depleted of NK cells. These T cells were combined with T cell-depleted, NK cell-depleted B10.S splenocytes and cultured with or without antigen for 3 d. Dendritic cells and NK cells were undetectable by FACS® analysis. Total RNA was extracted, and Northern blot analysis was performed for IL-12Rbeta 2 subunit and beta -actin. Results are representative of two experiments each using LN cells pooled from five or more mice.

IL-12Rbeta 2 Subunit mRNA Expression Correlates with Susceptibility to EAE.

We have previously demonstrated that the addition of pharmacological concentrations of IL-12 to cultures of B10.S MBP-reactive LN cells restored their ability to produce IFN-gamma and converted them into encephalitogenic effectors (28). Indeed, the addition of exogenous IL-12 restored the ability of the B10.S MBP-reactive LN cells to upregulate the expression of IL-12Rbeta 2 subunit mRNA in an antigen-specific, dose-dependent manner (Fig. 5 A). Although the addition of IFN-gamma failed to restore the ability of these cells to produce IFN-gamma upon secondary stimulation (28), modest induction of IL-12Rbeta 2 subunit expression was seen (data not shown). The addition of neutralizing antibodies to IL-12 or IFN-gamma to cultures of NP-reactive B10.S LN cells (Fig. 5 B) or MBP-reactive SJL LN cells (Fig. 5 C) inhibited the ability of these cells to upregulate IL-12Rbeta 2 subunit mRNA in response to antigenic stimulation. Thus, the complementary roles of endogenous IL-12 and IFN-gamma for optimal expression of IL-12Rbeta 2 subunit seen in the response of C57BL/6 mice to OVA can be extended to a second foreign antigen (NP) and an autoantigen (MBP).


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Fig. 5.   Maintenance of IL-12Rbeta 2 mRNA expression is dependent on the presence of IL-12 and IFN-gamma . (A) LN cells from B10.S mice coimmunized with MBP/NP were removed on day 12 after immunization and cultured for 3 d in the presence or absence of MBP with or without IL-12 (2 or 0.2 ng/ml). Total RNA was extracted, and Northern blot analysis was performed for IL-12Rbeta 2 subunit and beta -actin. Results are representative of three experiments each using LN cells pooled from five or more mice. (B) LN cells from B10.S mice coimmunized with MBP/NP were removed on day 12 after immunization and cultured for 3 d in the presence of NP with or without anti-IL-12 (10 µg /ml) or anti-IFN-gamma (10 µg/ml). Total RNA was extracted, and Northern blot analysis was performed for IL-12Rbeta 2 subunit and beta -actin. Results are representative of three experiments each using LN cells pooled from five or more mice. (C) LN cells from SJL mice coimmunized with MBP/NP were removed on day 12 after immunization and cultured for 3 d in the presence of MBP with or without anti-IL-12 (10 µg/ml) or anti-IFN-gamma (10 µg/ml). Total RNA was extracted, and Northern blot analysis was performed for IL-12Rbeta 2 subunit and beta -actin. Results are representative of three experiments each using LN cells pooled from five or more mice.

Role of IL-10 in the Expression of IL-12Rbeta 2 Subunit mRNA.

One possible explanation for the failure of B10.S MBP-specific T cells to induce IL-12 production and to upregulate IL-12Rbeta 2 expression is that a major component of the response to MBP in this strain is mediated by Th2 cells. However, we have previously failed to demonstrate production of IL-4 or IL-10 by B10.S MBP-specific T cells and treatment of B10.S mice with anti-IL-4 in vivo or anti-IL-10 in vitro did not result in the induction of IFN-gamma production (28). Furthermore, addition of anti-IL-4 in vitro failed to restore the ability of these cells to express IL-12Rbeta 2 subunit (data not shown). However, our recent demonstration that IL-10 production by antigen nonspecific CD4+ T cells regulates the proinflammatory effects of IL-12 in vivo prompted us to more carefully examine the role of IL-10 in the priming of B10.S mice to MBP in vivo (20). B10.S mice were immunized with MBP/NP and simultaneously treated with neutralizing anti-IL-10 mAbs. Neutralization of IL-10 in vivo and in vitro did not restore the ability of MBP-reactive LN cells to express IL-12Rbeta 2 subunit (Fig. 6 A) or to produce IFN-gamma (data not shown). However, it should be emphasized that IL-10 had a potent downregulatory effect on the expression the IL-12Rbeta 2 in vitro as the addition of exogenous IL-10 to cultures of either B10.S NP-reactive or SJL MBP-primed LN cells strongly inhibited upregulation of the expression of the IL-12Rbeta 2 subunit (Fig. 6, B and C).


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Fig. 6.   Treatment with anti-IL-10 does not reverse the defect in IL-12Rbeta 2 expression by B10.S MBP-reactive T cells. (A) B10.S mice coimmunized with MBP/NP were injected intraperitoneally with 1 mg of either control IgG or anti-IL-10 on days 0, 3, and 6. Draining LN cells were removed on day 12 after immunization and cultured for 3 d with antigen with either control IgG (10 µg/ml) or anti- IL-10 (10 µg/ml). Total RNA was extracted, and Northern blot analysis was performed for IL-12Rbeta 2 subunit and beta -actin. Results are representative of two experiments each using LN cells pooled from two or more mice. Addition of exogenous IL-10 in vitro inhibits the expression of IL-12Rbeta 2 subunit mRNA. (B) LN cells from B10.S mice coimmunized with MBP/NP were removed on day 12 after immunization and cultured for 3 d in the presence of NP with or without IL-10 (10 ng/ml). Total RNA was extracted, and Northern blot analysis was performed for IL-12Rbeta 2 subunit and beta -actin. Results are representative of three experiments each using LN cells pooled from five or more mice. (B) LN cells from SJL mice coimmunized with MBP/ NP were removed on day 12 after immunization and cultured for 3 d in the presence of MBP with or without IL-10 (10 ng/ml). Total RNA was extracted, and Northern blot analysis was performed for IL-12Rbeta 2 subunit and beta -actin. Results are representative of three experiments each using LN cells pooled from five or more mice.

The Failure of B10.S MBP-specific T Cells to Fully Differentiate into Th1 Effectors Does Not Appear to Be Secondary to a Decreased Functional Avidity of the TCR.

One of the problems with the comparison of immune responses between B10.S and SJL mice at the population level even with a well-defined peptide antigen is that these two strains differ by many background genes including the TCR gene complex (32). Thus, as a result of distinct positive/negative selection events in the thymus, the B10.S MBP-specific T cell repertoire may be quantitatively smaller or exhibit an overall lower affinity for the MBP87-106 peptide I-As complex compared with similarly restricted SJL T cells. The average lower affinity of the B10.S MBP-specific TCRs might result in impaired induction of CD40L and the cascade of events leading to defective upregulation of the IL-12Rbeta 2 subunit. Targoni and Lehmann have shown that immunization with low doses of peptide would generate recall responses only from T cells with a high affinity TCR for their MHC-peptide ligand (33). We immunized B10.S and SJL mice with different amounts (50, 100, or 400 µg) of MBP87-106 in CFA and measured T cell proliferation in response to a broad range of peptide concentrations in vitro (Fig. 7). In several experiments of this type, no consistent differences between SJL and B10.S mice were seen in the magnitude of the proliferative responses or the shape of the dose-response curves. Thus, it appears that the expansion of MBP-reactive cells is similar in the two strains and that no obvious difference exists in their functional avidity.


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Fig. 7.   B10.S mice were immunized with 50 (black-square), 100 (bullet ), or 400 µg (black-diamond ) of MBP87-106. SJL mice were immunized with 50 (square ), 100 (open circle ), or 400 µg (diamond ) of MBP87-106. On day 10, LN cells from each group were pooled (n = 2) and stimulated with concentrations of antigen ranging from 100 to 1 µg. Wells were pulsed with 1 µCi of [3H]thymidine at 72 h and harvested 16 h later. Background proliferation was subtracted. Results shown are representative of three experiments.

The Defect in IL-12Rbeta 2 Subunit Expression by B10.S MBP-specific T Cells Does Not Extend to Other Myelin Autoantigens.

Several studies have suggested that B10.S mice have a global defect in their responses to myelin autoantigens as the incidence and/or severity of EAE in B10.S mice immunized with PLP139-151 or with whole spinal cord homogenate was significantly less than that seen in similarly immunized SJL mice. To examine whether a failure of IL-12Rbeta 2 subunit expression was also seen in response to PLP, we immunized B10.S mice with a combination of MBP and PLP in CFA. PLP- but not MBP-reactive LN cells upregulated IL-12Rbeta 2 subunit strongly in response to antigenic stimulation (Fig. 8 A). Furthermore, PLP- but not MBP-reactive LN cells produced large quantities of IFN-gamma in response to antigenic stimulation in secondary cultures (Fig. 8 B). To further address the question of whether the encephalitogenicity of primed LN cells correlated with their ability to express the IL-12Rbeta 2 subunit, we immunized B10.S mice with PLP139-151 or MBP87-106 in CFA. 10 d later, we stimulated draining LN cells in vitro with PLP or MBP for 4 d, then transferred the cells into naive B10.S recipients. Transfer of PLP-reactive LN cells resulted in a 30% incidence of EAE, whereas transfer of MBP-reactive LN cells failed to induce EAE in any mice (Fig. 8 C). Thus, the capacity of LN cells to transfer EAE was found to correlate with their ability to express IL-12Rbeta 2 subunit upon activation.


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Fig. 8.   The ability of PLP-reactive LN cells to express IL-12Rbeta 2 subunit mRNA correlates with their capacity to transfer EAE. (A) B10.S mice were immunized with a combination of MBP and PLP. On day 12, draining LN cells were removed and cultured with antigen for 3 d. Total RNA was extracted, and Northern blot analysis was performed for IL-12Rbeta 2 subunit and beta -actin. Results are representative of three experiments each using LN cells pooled from five or more mice. (B) B10.S mice were immunized with either MBP or PLP. On day 12, draining LN cells were removed and cultured with antigen for 4 d. Cells were extensively washed and restimulated with or without antigen for 48 h, and IFN-gamma production was assayed by ELISA. Results are representative of two experiments each using LN cells pooled from 10 or more mice. (C) B10.S mice were immunized with MBP/PLP. On day 10, draining LN cells were removed and cultured with antigen for 4 d. The cells were then washed and injected (6 × 107 cells intraperitoneally per mouse) into naive recipients. Animals were monitored daily for neurological signs. Results shown are representative of two experiments performed with at least eight mice in each group.

    Discussion
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

Although CD4+ Th1 lymphocytes have been implicated as the effector cells in both animal models and human organ-specific autoimmune diseases, the failure to identify a single effector cytokine that is responsible for pathogenicity has suggested that cytokine-targeted therapeutic approaches to autoimmune disease should be directed against the primary cytokine responsible for the differentiation of Th1 cells, IL-12. Because the IL-12Rbeta 2 subunit has been identified as the critical molecule involved in maintaining IL-12 responsiveness and in controlling Th1 lineage commitment, we have focused our studies on critically examining the requirements for induction of the IL-12Rbeta 2 subunit on CD4+ T cells responding to autoantigens as well as conventional foreign antigens. We have used cytokine-deficient mice to establish a critical role for IL-12 itself, independent of IFN-gamma , in the induction of IL-12Rbeta 2 expression. Although IFN-gamma was capable of upregulating IL-12Rbeta 2 on T cells derived from IL-12-/- mice, the level of expression was always less than that seen on T cells from wild-type and IFN-gamma -/- mice. As IL-12-/- mice have been found to have markedly deficient Th1 responses and to be resistant to Th1-mediated autoimmune diseases (34), the significance of this IL-12-independent, IFN-gamma -dependent pathway of IL-12Rbeta 2 upregulation remains to be determined. More importantly, our studies with neutralizing antibodies in vitro have clearly shown that both IL-12 and IFN-gamma play critical roles in the upregulation of IL-12Rbeta 2 on restimulation of antigen-primed T cells in vitro from wild-type mice.

We have previously defined a critical role for IL-12 in the cytokine cascade needed for activation of encephalitogenic MBP-specific T cells from EAE-resistant B10.S mice (28). Although we could induce Th1 differentiation with pharmacological concentrations of IL-12, we did not determine whether the failure to normally generate MBP-specific Th1 cells in this strain involved deficient IL-12 production or deficient IL-12Rbeta 2 subunit expression. We have now shown that the primary defect in the immune response of the B10.S mouse to MBP is defective expression of CD40L on the MBP-specific T cell population with subsequent failure to generate IL-12 production from APCs. This in turn leads to defective expression of sufficient levels of IL-12Rbeta 2 needed for Th1 differentiation. The importance of CD40/CD40L interactions in the differentiation of encephalitogenic effectors is supported by the suppression of EAE induction by the administration of blocking CD40L mAbs (35, 36). Our previous studies have ruled out excessive production of IL-4 as the mechanism responsible for defective MBP-specific Th1 differentiation in the B10.S mouse (28). Neutralization of IL-10 in vivo and in vitro also did not restore IL-12Rbeta 2 expression by the MBP-specific T cells. However, addition of exogenous IL-10 to the in vitro culture exerted a powerful downregulatory influence on expression of IL-12Rbeta 2 subunit by antigen-specific T cells primed in vivo under Th1 conditions. Presumably, IL-10 acted directly on the APCs in the cultures to inhibit IL-12 production and thereby mimicked the effects of the addition of anti-IL-12 (37). However, a direct effect of IL-10 on the responding T cells remains a possibility. In any case, it appears that once IL-12Rbeta 2 subunit expression is induced by IL-12 in vivo, IL-10 can act to limit the level of functional IL-12Rbeta 2 ultimately expressed. Indeed, this step may be the most important therapeutic effect of IL-10 in the treatment of autoimmune disease.

The antigen-specific defect we have observed in the CD40L/IL-12/IL-12Rbeta 2 subunit pathway in MBP-specific B10.S T cells should be contrasted with the more global defects of the B10.S strain in the response to myelin- derived autoantigens reported by others (38, 39). It is likely that this antigen-specific defect is superimposed over and above a more global defect. First, although the addition of exogenous IL-12 to cultures of B10.S MBP-reactive T cells restored their capacity to transfer EAE, the resultant disease was monophasic and less severe than the relapsing-remitting course manifested by SJL recipients of syngeneic MBP-primed T cells (28). Second, although we have demonstrated that B10.S PLP-reactive T cells can induce disease upon adoptive transfer, it should be noted that disease incidence and severity were much less than those observed in SJL mice. Furthermore, production of IFN-gamma was only observed during secondary but not primary stimulation in vitro. The differences between our results and those of Encinas et al., who reported complete resistance of B10.S mice to PLP-induced EAE, may be secondary to the different disease induction protocols used (active induction versus passive transfer) or differences in the mouse colonies consequent to genetic drift (38). Thus, B10.S mice appear to have inherited a set of traits that confer a certain degree of protection against autoimmune phenomena in general, distinct from the antigen-specific defect in the IL-12 pathway. This set of traits is presumably mediated by the products of non-H-2 background genes and may also be responsible for the monophasic (as opposed to relapsing- remitting) course of EAE exhibited by B10.PL mice as well as for the resistance of C57BL/6 mice to autoimmune orchitis. In this context, it is interesting to note that one of the genetic loci found to be important in susceptibility to EAE (eae7) colocalizes to the same region of chromosome 11 as Orch3, a susceptibility locus in autoimmune orchitis (39). Hence, in the case of the B10.S response to MBP, we believe that absolute resistance arises as a result of the antigen-specific defect superimposed on a more global pattern of resistance to the manifestations of autoimmune disease.

What then is the mechanism responsible for the generation of this antigen-specific defect in the capacity of a population of autoreactive T cells to differentiate into pathogenic Th1 effector cells? The simplest explanation is that it is secondary to the size of the T cell repertoire specific for MBP. We believe that this is unlikely as the magnitude of the proliferative responses and of IL-2/IL-3 production by B10.S mice to MBP does not differ significantly from that of SJL mice (28). However, this question will only be able to be addressed quantitatively when specific peptide-MHC binding T cells are measured in this model. It is also possible that the affinity of the interaction of the anti-MBP TCRs from B10.S mice with the MBP peptide-I-As complex is too low to generate a sufficient signal to trigger high enough levels of CD40L expression to trigger the IL-12 production cascade. However, we detected no differences between the proliferative capacities of B10.S and SJL MBP87-106 reactive populations after immunization with limited doses of peptide, suggesting that there are no major differences in TCR avidity. It is also unlikely that the failure of the B10.S T cells to differentiate is secondary to the delivery of a suboptimal costimulatory signal which potentially could influence CD40L expression, as the addition of anti-CD28 to B10.S cells in vitro failed to generate antigen- specific IFN-gamma production (our unpublished observations).

We propose that the defect in the B10.S MBP-specific population is generated during the process of negative selection during thymic differentiation. Two recent studies in shiverer mice, which have a mutation in the MBP gene, have suggested that those autoantigen-specific T cells that normally escape negative selection in the thymus to enter the peripheral T cell pool do so because of their low affinity for the autoantigen (33, 40). Yet, MBP87-106-specific T cells from B10.S mice appear to have emerged from the negative selection process with an additional defect---a blunted capacity to become pathogenic effectors even when primed under conditions that readily generate such effectors in susceptible strains of mice such as the SJL or B10.PL. We have previously postulated that one mechanism by which autoreactive T cells may escape deletion in the thymus is secondary to complete downregulation of their TCR signaling properties with a major defect in their capacity to produce IL-2 (41). Such cells, which are characterized by expression of the CD25 antigen, appear to function as suppressor cells that prevent organ-specific autoimmunity after thymectomy early in life. The B10.S MBP-specific cells appear to be the products of a different fail-safe mechanism imposed on the autoreactive T cell repertoire during negative selection---the inability to upregulate the CD40L antigen and the subsequent IL-12 cascade. Thus, negative selection of autoreactive TCRs may be a more complex process than simple killing of high affinity cells and allowing low affinity cells to pass through. Intrathymically, some autoreactive T cells may recognize their target autoantigen in a qualitatively different way similar to the manner in which mature T cells recognize altered peptide ligands. However, at this stage of their development, this altered recognition of self may result in a variety of permanent defects in the TCR signal transduction cascade ranging from anergy to the partial defect we have described in the MBP-reactive population of the B10.S mouse. This model does not exclude the possibility that the expression of autoantigens, such as MBP, in the periphery may also be required for the maintenance of the nonpathogenic state of the autoreactive T cells. We suspect that other interesting phenotypes involving the inability of autoreactive cells to fully differentiate will be uncovered in the future.

What are the implications of these results for the pathogenesis of organ-specific autoimmune disease in experimental animals and in man? B10.S MBP-reactive T cells defy simple categorization into the Th1/Th2 paradigm. They differ from Th1 cells by failing to upregulate IL-12Rbeta 2 to an appreciable degree upon activation. However, they also differ from Th2 cells by retaining the capacity to express IL-12Rbeta 2 when triggered under conducive environmental conditions. B10.S MBP-reactive cells fall within a distinct subset of Th cells, which we refer to as Tpre-A cells, signifying a latent ability to develop into autoimmune effectors. We have found that IL-12-inducing microbial components or high doses of recombinant IL-12 can serve as triggers for the conversion of Tpre-A cells (27, 28). This may explain, in part, why infectious diseases are so effective in precipitating the onset and exacerbation of autoimmune diseases, including multiple sclerosis (MS). Tpre-A cells may comprise a significant number of autoreactive T cells in the periphery of healthy individuals. Hence, defining those conditions that favor as well as suppress their conversion into mature autoimmune effectors could have important clinical implications regarding the prevention of autoimmune disease and its recurrences. Similar numbers of myelin protein-reactive T cells have been detected in the peripheral blood of MS patients and healthy HLA-matched controls (42). The antigen-specific inducibility of the IL-12Rbeta 2 subunit and/or CD40L may potentially serve as more reliable distinguishing characteristics of pathogenic myelin protein-reactive T cells from MS patients. If a recurrent pattern is established, IL-12Rbeta 2 subunit or CD40L expression may eventually serve as a marker to detect subclinical disease and measure active disease in MS. Furthermore, the development of immunotherapies that target subsets of T cells expressing high levels of the IL-12Rbeta 2 subunit may be warranted.

    Footnotes

Address correspondence to Ethan M. Shevach, LI, NIAID, NIH, Bldg. 10, Rm. 11N311, 10 Center Dr.- MSC 1892, Bethesda, MD 20892-1892. Phone: 301-496-6449; Fax: 301-496-0222; E-mail: ems1{at}box-e.nih.gov

Received for publication 20 November 1998 and in revised form 25 January 1999.

   J.T. Chang is a Research Scholar of the Howard Hughes Medical Institute.

Abbreviations used in this paper CD40L, CD40 ligand; CIA, collagen- induced arthritis; EAE, experimental allergic encephalomyelitis; MBP, myelin basic protein; NP, nucleoprotein; PLP, proteolipid protein.

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Results
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