By
From the * Laboratoire d'Immunologie Cellulaire, Unité de Recherche Associée, Centre National de la
Recherche Scientifique 625, Bâtiment Centre d'Études et de Recherches en Virologie et Immunologie,
Hôpital Pitié-Salpétrière, 75013 Paris, France; UMR 146 Centre National de la Recherche
Scientifique, Institut Curie, 91405 Orsay, France; the § Biomedical Research Centre, University of
British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada; and the
Theodor Kocher
Institute, University of Bern, CH-3000 Bern 9, Switzerland
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Abstract |
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CC chemokines produced by CD8+ T cells are known to act as HIV-suppressive factors. We
studied the possible role of these chemokines in HIV-1-specific killing of target cells. We found
that the activity of cytotoxic T lymphocytes (CTLs) in CTL lines or freshly isolated peripheral
blood mononuclear cells from HIV-1-infected individuals is markedly enhanced by RANTES
(regulated on activation, normal T cell expressed and secreted) and virtually abolished by an antibody neutralizing RANTES or the RANTES receptor antagonist RANTES(9-68). Lysis was
mediated by CD8+ major histocompatibility complex class I-restricted T cells and was obtained
with target cells expressing epitopes of the HIV-1LAI proteins Gag, Pol, Env, and Nef. The cytolytic activity observed in the presence or absence of added RANTES could be abolished by
pretreatment of the CTLs with pertussis toxin, indicating that the effect is mediated by a G protein-coupled receptor. The chemokines monocyte chemotactic protein (MCP)-3, MCP-4, and
eotaxin acted like RANTES, whereas macrophage inflammatory protein (MIP)-1, MIP-1
,
MCP-1, and stromal cell-derived factor 1 were inactive, suggesting a role for the eotaxin receptor, CCR3, and ruling out the involvement of CCR1, CCR2, CCR5, and CXCR4. CTL activity was abrogated by an antibody that blocks CCR3, further indicating that specific lysis is
triggered via this chemokine receptor. These observations reveal a novel mechanism for the induction of HIV-1-specific cytotoxicity that depends on RANTES acting via CCR3.
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Introduction |
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irus-specific cytolysis is considered a major defense function of CD8+ T lymphocytes that eliminate productively infected cells and control the viral load during the asymptomatic stages after HIV infection (1). HIV-1-specific CTLs have been studied extensively, and it has been shown that they are present in high numbers in the blood and and tissues (2). However, specific CTL activity declines with the development of AIDS (5, 6). CD8+ T cells are an important source of chemokines that were shown to inhibit HIV entry into CD4-bearing cells and to limit infection in vitro (7) and in vivo (8). On the other hand, chemokines are potent attractants of T lymphocytes and NK cells (9) and could, therefore, enhance antiviral effector functions by stimulating cytotoxicity. This action would differ from the well-established role of some chemokines as competitors for HIV coreceptor usage (10).
Monitoring of specific cytolytic activity is a way to assess the antiviral defense of HIV-1-infected individuals (11, 12). Activated, CD8+ HIV-1-specific CTLs are known to produce interferons and chemokines (13), but other factors or mechanisms may contribute to their overall HIV-1-suppressive activity (7, 14). We have now studied the effects of chemokines on PBMCs and PBMC-derived, HIV-1-specific CTL lines generated from HIV-1-infected individuals who were monitored for several years. We present data indicating that the HIV-1-specific lytic activity of patient-derived CTLs is enhanced by RANTES (regulated on activation, normal T cell expressed and secreted), which acts on the CTLs through the chemokine receptor CCR3.
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Materials and Methods |
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HIV-1-infected Donors.
The donors of PBMCs used to establish CTL lines and target cells were eight HLA-typed, HIV-1-infected patients from the French cohort IMMUNOCO with CD4+ T lymphocyte counts between 150 and 1,050/µl. They were recruited in 1991 and were followed for 5 yr on the basis of clinical and functional tests.Generation of HIV-1-specific CTL Lines.
CTL lines were prepared according to established methods (11, 12). PBMCs were isolated from the venous blood of HIV-1-infected individuals by centrifugation in Ficoll-Hypaque density gradients (Eurobio, Les Ulis, France) and were activated overnight with 1 µg/ml PHA (HA-16; Murex Diagnostic Ltd., Dartford, England). Polyspecific CTL lines were generated by coculture with irradiated autologous PHA blasts, and CTL lines specific for the Pol epitope of HIV-1LAI (amino acid 476-484) by coculture with irradiated autologous PHA blasts that were pre-incubated for 2 h at 37°C with 10 µM of the synthetic peptide 476-484 (15). The appropriate irradiated PHA blasts were added every tenth day, and the cocultures were performed in the presence of recombinant human IL-2 (20 U/ml; Boehringer Mannheim, Germany) supplied every third day. The CTLs were used for the cytotoxicity assays after 20 d of coculture.Target Cells.
Three types of target cells were used: autologous EBV-transformed lymphoblastoid B cell lines infected with vaccinia virus expressing the HIV-1LAI proteins Gag, Pol, or Env; autologous EBV-transformed lymphoblastoid B cell lines pulsed with the synthetic peptide 476-484 corresponding to the Pol epitope of HIV-1LAI; and the human plasmocytoid cell line Hmy cotransfected with the HLA-A2 and the HIV-1LAI nef genes. The EBV-transformed lymphoblastoid B cell lines were established for each CTL donor. Infection with wild-type vaccinia virus or with vaccinia virus expressing the HIV-1LAI proteins Gag, Pol, or Env (Transgene, Strasbourg, France) was performed for 18 h at 37°C at a multiplicity of 5 PFU/cell. Pulsing was performed with 10 µM of the synthetic peptide 476-484 for 2 h at 37°C using EBV-transformed cells already labeled with 51Cr (12). EBV-transformed cells infected with wild-type vaccinia virus or not pulsed with the synthetic Pol peptide and Hmy cells transfected with the HLA-A2 gene alone were used as the corresponding controls. The Hmy cell lines transfected with the HLA-A2 or HLA-A2 and the HIV-1LAI nef genes (16, 17) were provided by Dr. W. Biddison (National Institutes of Health, Bethesda, MD).HIV-1-specific CTL Activity.
The target cells were labeled for 2 h at 37°C with 100 µCi per 106 cells Na51Cr (Amersham, Les Ulis, France), and washed twice with culture medium. The target cells were distributed in round-bottomed 96-well microtiter plates (4 × 103 cells per well), and the effector cells were added at E/T ratios ranging between 120:1 and 3:1. The plates were centrifuged at 300 rpm for 2 min and incubated for 4 h at 37°C. The supernatants were then collected and 51Cr-release was measured in a gamma counter. The relative specific 51Cr-release was calculated as previously described (12). Values for spontaneous 51Cr-release, which are deducted in the calculation, were between 10 and 20% of the total incorporated radioactivity. The results are presented after subtraction of the nonspecific lysis obtained with control targets. All experiments were performed in triplicate. SE of triplicates was always <5% of the mean value and was omitted for clarity.NK and Lymphokine-activated Killer Cell Generation and Activity.
PBMCs from healthy donors were isolated by centrifugation in a Ficoll-Hypaque density gradient and were immediately tested for NK activity on K562 target cells. Alternatively, the PBMCs were cultured for 48 h with 100 U/ml of recombinant IL-2 and tested for lymphokine-activated killer (LAK) activity on Daudi target cells. The experiments were performed at E/T ratios ranging between 70:1 and 7:1. The conditions for the Cr release assays were the same as for the experiments with CTLs.Flow Cytometry.
CCR3 expression was assessed on effector and target cells using anti-CCR3 (see Reagents). For double fluorescence analysis of PBMCs, 5 × 105 cells were incubated for 30 min at 4°C with 10 µg of anti-CCR3, washed with PBS, fixed with 1% paraformaldehyde in PBS, and analyzed on a FACScan® flow cytometer (Becton Dickinson, San Jose, CA). 104 events per sample were collected and analyzed using the Cellquest software (Becton Dickinson).Reagents.
RPMI 1640 medium (Gibco Life Technologies, Cergy Pontoise, France) supplemented with 10% heat-inactivated FCS, and standard concentrations of L-glutamine, sodium pyruvate, penicillin, and streptomycin was used as the medium in all cultures and for dissolving additions. The chemokines and the chemokine antagonist RANTES(9-68) were prepared by chemical synthesis (18). If not stated otherwise they were added to the assays at the time of mixing effector and target cells. RANTES determination in cell supernatants was performed by ELISA (R&D Systems, Minneapolis, MN) and the data were analyzed using the Softmax program (Molecular Devices, Sunnyvale, CA). A standard concentration curve was assayed with each analysis. Four neutralizing monoclonal antibodies were used: anti-RANTES (R&D Systems, Minneapolis, MN); anti-CCR3 (7B11; LeukoSite, Cambridge, MA); anti-CD4 (IOT4; Immunotech, Marseille, France); and anti-CD8 (B9-11; Immunotech). An anti-VLA6 monoclonal of the same isotype (Immunotech) was used as irrelevant antibody control. The antibodies were added to the suspension containing the CTLs shortly before mixing with the targets at the concentration of 1 µg/ml, which was found, in concentration-dependence assays, to be sufficient for neutralization. Pretreatment of CTLs with Bordetella pertussis toxin (Sigma-Aldrich, St. Quentin Fallavier, France) was performed for 1.5 h at 37°C in the standard medium, followed by washing. ![]() |
Results and Discussion |
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The effect of RANTES on HIV-1-specific cytotoxicity was examined using polyspecific MHC class I-restricted CTL lines established from HIV-1-infected donors that recognize the HIV-1LAI Env, Pol, Gag, or Nef gene products. As shown in Fig. 1 A, the specific lysis of autologous B cell lines expressing Gag, was markedly enhanced by RANTES and was suppressed by a RANTES-neutralizing antibody. Similar effects were obtained when the lytic activity of patient-derived HIV-1- specific CTLs was tested with two other targets, autologous B cells pulsed with a HIV-1 Pol peptide (Fig. 1 B) and a human plasmocytoid cell line, Hmy, cotransfected with the HLA-A2 and the HIV-1LAI nef genes (Fig. 1 C). By contrast, neither RANTES nor the RANTES-neutralizing antibody influenced the lysis mediated by blood-derived NK or LAK cells, as illustrated for NK cells in Fig. 1 D. As shown in Fig. 1 E, the enhancing effect of RANTES and the inhibition of CTL activity by either neutralizing RANTES or blocking its receptor with the antagonist RANTES(9-68) were regularly observed with several CTL lines specific for Gag, Pol, or Env derived from HIV-1- infected individuals. Together these results indicate that RANTES was required for the lytic activity of HIV-1-specific CTLs of the cohort patients. Comparison of CTLs from different individuals (Fig. 1 E) shows that the effects were similar for different HIV-1 epitopes presented by the target cells, and were highly reproducible. The use of different types of target cells (Fig. 1, A-C) indicates that the procedure used to induce expression of HIV-1-specific epitopes had no influence on the outcome of the experiments.
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The effects of RANTES and RANTES(9-68) were concentration dependent (Fig. 2 A). Specific lysis was nearly
doubled at 10 nM RANTES and approached a maximum
between 50 and 100 nM, whereas inhibition by RANTES
(9-68) was observed above 25 nM and was nearly complete
at 50 and 100 nM. On the other hand, no effect was obtained with increasing concentrations of macrophage inflammatory protein (MIP)-1. The concentrations shown
are similar to those required for leukocyte activation by
chemokines and the inhibition of functional responses by
chemokine antagonists (19). Since chemokine antagonists have lower receptor affinity than the chemokines
themselves (20), it is not surprising that higher concentrations of RANTES(9-68) were required to achieve a biological effect. As shown in Fig. 2 B, pretreatment of the
CTLs with increasing concentrations of pertussis toxin progressively decreased the cytolytic activity observed in the
presence of RANTES, indicating that the chemokine effect is mediated by a G protein-coupled receptor (9). Pertussis toxin also abrogated CTL activity in the absence of
added RANTES (data not shown). To identify the cells responding to RANTES, effector and target cells were pretreated separately before testing cytotoxicity (Fig. 2 C).
Pretreatment of the effector cells with RANTES yielded
similar results as shown in Fig. 1 when the chemokine was
added at the time of mixing effectors and targets. By contrast, no changes were observed when the same pretreatment was applied to the target cells. MIP-1
and MIP-1
,
which are produced by CD8+ T cells together with
RANTES (22, 23), were ineffective, as shown in Fig. 2 C
for MIP-1
, suggesting that MIP-1
and MIP-1
do not
share the relevant receptor with RANTES. These results indicate that RANTES acts on the CTLs and has no toxic
or other apparent effect on the target cells. Additional support for this conclusion comes from the observed inhibition of the effect of RANTES after pretreatment of the
CTLs with pertussis toxin (Fig. 2 B).
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RANTES binds to
several receptors. It attracts monocytes (19, 24) and T lymphocytes (25) via CCR1, and eosinophils and basophils via
CCR3 (26, 27) and acts on T lymphocytes via CCR5 (28).
CCR4 was also reported as a candidate receptor (29), but
was later shown to bind a novel CC chemokine called
TARC (30). To identify the receptor involved in HIV-1-
specific CTL activity, we tested the effect of several well-characterized chemokines. As shown in Fig. 3, RANTES
could be substituted by eotaxin, monocyte chemotactic
protein (MCP)-3 and MCP-4, but not by MIP-1, MCP-1, or stromal cell-derived factor (SDF)-1. This suggests that
the observed HIV-1-specific CTL activity depends on
CCR3 and rules out effects via CCR1, CCR2, CCR5,
and CXCR4. To definitively prove the involvement of
CCR3, we used a monoclonal antibody that selectively blocks the function of this receptor (26). Fig. 3 shows that target cell lysis was abrogated in the presence of the antibody confirming that HIV-1-specific cytotoxicity is mediated by
chemokines that act via CCR3. Anti-CCR3 also prevented
the lysis of autologous B cells pulsed with a HIV-1 Pol peptide
476-484 and of the Hmy plasmocytoid cells expressing HLA-A2 and HIV-1LAI Nef, but had no effect on the lysis by NK
cells (data not shown). We tested the expression of CCR3 in
several HIV-1-specific CTL lines established from HIV-1-
infected individuals. The receptor was detectable in all lines,
and the percentage of positive cells varied between 3 and 10 depending on the donor. In no instance CCR3 was detected on the target cells (data not shown). Although several
chemokines that bind to CCR3 enhanced CTL activity,
RANTES appears to be the biologically relevant stimulus,
since lysis was virtually abolished when this chemokine was
selectively neutralized with the anti-RANTES antibody. It
has been previously reported that CD8+ T cells produce
RANTES and other CC chemokines (31). We have determined the production of RANTES by two CTL lines for
the duration of the cytotoxicity assay at an E/T ratio of 120:1,
and found concentrations of 52 ± 5 and 138 ± 22 pg/ml ± SD in the culture medium under conditions that yielded 20 and 40% specific lysis, respectively. These concentrations are
considerably lower than those added in this study to enhance
lysis. The low level of RANTES detected in these assays may
be related to the weak lytic activity observed even at high E/T
ratios. On the other hand, RANTES is likely to bind to CCR3
as it is released by the producing cells, and the assay in the
supernatant may underestimate the effective concentrations.
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After having shown that RANTES is a major mediator of lysis by CTL lines derived from HIV-1-infected patients, it was important to assess whether the activity of freshly isolated PBMCs was also RANTES-dependent. As shown in Fig. 4, HIV-1-specific lysis by PBMCs that were freshly isolated from infected individuals was enhanced by RANTES and virtually abolished by antibodies that neutralize RANTES or block CCR3. In addition, Fig. 4 shows that CTL lines established from the PBMCs of both donors lysed autologous but not mismatched targets. The lytic activity was abrogated by anti-CD8 antibodies, both in the presence and absence of RANTES indicating that it was mediated by CD8+ T lymphocytes. Anti-CD4 antibodies, by contrast, were without effect (data not shown).
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This study shows that RANTES mediates the HIV-1-
specific killing of target cells by MHC-restricted CD8+ T
lymphocytes through a mechanism that depends on the
chemokine receptor CCR3. It is of interest that RANTES
is produced by CD8+ T cells after antigen-specific stimulation (35) and that CCR3 is expressed in CD4+ and CD8+
T lymphocytes (36). MIP-1, MIP-1
, RANTES, and
MCP-1 were reported to enhance the proliferation of human T cells in response to anti-CD3 and of T cell clones
challenged with the relevant antigen (37), suggesting a role
of CC chemokines in the activation of polyclonal and antigen-specific cytotoxicity (38). A costimulatory function of
RANTES cannot be ruled out although the selectivity observed in this study suggests a different mechanism. Our
observations reveal a new facet of the antiviral properties of
RANTES. The involvement of CCR3 suggests the possibility to enhance its expression by CD8+ effector T cells in
order to prevent the decline of CTL activity in AIDS and
to improve cellular defense in other immunodeficiencies. In addition, retroviral gene transduction approaches could
be used to regulate the endogenous expression of
RANTES or RANTES-inducing cytokines.
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Footnotes |
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Address correspondence to Patrice Debré, Laboratoire d'Immunologie Cellulaire, Bâtiment CERVI, Hôpital Pitié-Salpétrière, Boulevard de l'Hopital 83, 75013 Paris, France. Phone: 33-1-4217-7482; Fax: 33-1-4217-7490; E-mail: patrice.debre{at}psl.ap-hop-paris.fr
Received for publication 29 April 1998 and in revised form 29 May 1998.
We are particularly indebted to Dr. Fernando Arenzana (Institut Pasteur, Paris) and Beatrice Dewald (Theodor Kocher Institute, Bern) for discussions and important suggestions.This study was supported by the Agence Nationale pour la Recherche sur le Sida (ANRS), the Swiss National Science Foundation (Grant 31-39744.93), and the Protein Engineering Network of Centers of Excellence (PENCE), Canada.
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