Correspondence to: Kensuke Miyake, Department of Immunology, Saga Medical School, Nabeshima, Saga 849-8501, Japan. Tel:81-952-34-2256 Fax:81-952-34-2049 E-mail:miyake{at}post.saga-med.ac.jp.
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Abstract |
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The susceptibility to infections induced by Gram-negative bacteria is largely determined by innate immune responses to bacteria cell wall lipopolysaccharide (LPS). The stimulation of B cells by LPS enhances their antigen-presenting capacity and is accompanied by B cell proliferation and secretion of large quantities of LPS-neutralizing antibodies. Similar to macrophages and neutrophils, the LPS-induced activation of B cells is dependent on Toll-like receptor (TLR)4. Here, we demonstrate that the responses of B cells to LPS are also regulated by another TLR protein, RP105, which is predominantly expressed on mature B cells in mice and humans. The analysis of mice homozygous for the null mutation in the RP105 gene revealed impaired proliferative and humoral immune responses of RP105-deficient B cells to LPS. Using originally LPS-unresponsive Ba/F3 cells expressing exogenous TLR4 and RP105, we demonstrate the functional cooperation between TLR4 and RP105 in LPS-induced nuclear factor B activation. These data suggest the existence of the TLR4RP105 signaling module in the LPS-induced B cell activation.
Key Words: gene targeting, lymphocyte, activation, leucine-rich repeat, cell surface molecule
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Introduction |
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A large body of evidence supports the important role of B cells in host responses against Gram-negative bacteria. These responses are largely triggered by bacteria cell wall LPS. Stimulation of B cells by LPS enhances their antigen-presenting capacity and is accompanied by B cell proliferation and secretion of large quantities of LPS-neutralizing Abs (1) (2). In turn, LPS-neutralizing Abs are important for clearing LPS and Gram-negative bacteria out of the circulation (3). The responses of B cells to LPS are mediated by cell surface Toll-like receptor (TLR)1 4, which belongs to the multimember family of mammalian proteins homologous to Toll protein of Drosophila melanogaster (4) (5). The TLR proteins possess an extracellular leucine-rich repeat (LRR) domain, a single transmembrane domain, and a cytoplasmic domain homologous to the cytoplasmic portion of the IL-1 receptor (5). The significance of TLR4 in B cell activation by LPS was underscored by profoundly reduced in vitro and in vivo LPS responses of B cells harboring spontaneous or genetically engineered null mutations in the TLR4 gene (6) (7) (8). The LPS signaling via TLR4 is dependent on association of the LRR domain of TLR4 with the MD-2 protein produced by TLR4-expressing cells (9).
The essential role of TLR4 in B cell responses to LPS is contrasted by the relatively low expression levels of the TLR4MD-2 complex on the surface of ex vivoisolated mature B cells compared with peritoneal macrophages. Using the MTS510 mAb that in flow cytometry analyses recognizes TLR4 in association with MD-2 but not TLR4 alone, we found the expression levels of TLR4MD-2 on B cells to be >10 times lower than on the peritoneal thioglycollateelicited macrophages (10). The hardly detectable expression of TLR4MD-2 on B cells suggests the possible cooperation between TLR4 and other TLR protein(s) in B cell signaling of LPS. In addition to TLR4, B cells express another member of the TLR family, the RP105 protein (11) (12) (13). In contrast to TLR4, which is expressed on immune cells of different types, the expression of RP105 is largely restricted to mature peripheral B cells (11). RP105 is associated with the MD-2 homologous protein MD-1 (14) (15). Robust B cell proliferation and upregulation of the cell surfaceexpressed MHC class II and costimulatory proteins B7.1 and B7.2 induced by Ab-mediated cross-linking of RP105 revealed this TLR as a potent regulator of B cell activation (11) (16). To test whether RP105 controls B cell responses to LPS, as well as to address the possible role of RP105 in B cell development and activation, we have generated mice homozygous for the null mutation of the RP105 gene. Using mice deficient for RP105, we revealed an important role of RP105 in B cell activation by LPS in vitro and in vivo.
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Materials and Methods |
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Generation of the RP105-deficient Mice.
The DNA clone containing the RP105 gene was obtained from Genome Systems, Inc., and the RP105 gene was subcloned into the pBluescript vector (Stratagene). For the construction of the RP105 gene-targeting vector, the 2.4-kB fragment of the exon 3 of the RP105 gene was replaced by the neomycin resistance (neor) gene under the control of the mouse phosphoglycerate kinase (PGK)-1 gene promoter. The HSV thymidine kinase gene driven by the PGK-1 promoter was introduced at the 3' end of the targeting vector. The linearized DNA of the targeting vector was transfected into the E14.1 embryonic stem (ES) cells, followed by selection of the G418- and gancyclovir-resistant ES cell clones as described previously (17). The clones of double-resistant cells were collected and genotyped by Southern blot analysis using the probe located outside the targeting construct. Cells of three independent recombinant ES clones were used to produce chimeric mice, followed by the generation of heterozygous RP105+/- and homozygous RP105-/- mice. The RP105+/- mice were backcrossed four times to C57BL/6 mice and RP105-/- mice (F5) at 615 wk of age, and were used for most of the experiments. The wild-type littermates derived from the breeding of the RP105+/- mice were used as control. C3H/HeJ and C3H/HeN mice were obtained from Japan SLC, Inc. Mice were maintained in the animal facilities at Saga Medical School and Institute for Genetics.
Cell Staining and Flow Cytometry.
Single cell suspensions were prepared from different lymphoid organs and incubated at 106 cells per 20 µl on ice in staining buffer (PBS containing 0.5% BSA and 0.01% NaN3) with optimal amounts of FITC-, PE-, or biotin-conjugated Abs for 10 min. The following mAbs were purchased from BD PharMingen: S7 (anti-CD43), B3B4 (anti-CD23), and Ly1 (anti-CD5). The following Abs were prepared: RA3-6B2 (anti-B220), R33-24.12 (anti-IgM), 1.3-5 (anti-IgD), and Cfo-1 (anti-Thy1.2). Flow cytometric analysis was performed on a FACScanTM cytometer (Becton Dickinson).
Analysis of B Cell Proliferation In Vitro.
Splenic B cells were purified by negative selection with the anti-CD43 mAb S7 conjugated either to Dynabeads M-450 sheep antirat IgG (Dynal) or to CD43-coupled magnetic beads (Miltenyi Biotec) as described (18). B cell purity has been always >95%, as judged by flow cytometry analyses (data not shown). The wild-type and mutant B cells were incubated at 2 x 105 per well in 96-well flat-bottomed plates with goat antimouse IgM F(ab')2 (Organon Teknika) in the presence or absence of recombinant mouse IL-4 (R&D Systems) or of rat antimouse CD40 mAb (LB429S; a gift from Dr. Nobuo Sakaguchi, Kumamoto University School of Medicine, Kumamoto, Japan). For the analysis of LPS responses, B cells were treated with LPS derived from Escherichia coli 0111:B4 (Sigma-Aldrich), E. coli 055:B5 (Sigma-Aldrich), or Salmonella minnesota Re595 (Sigma-Aldrich). Lipid A was either of bacterial (S. minnesota) or synthetic (a gift from Dr. Teruo Kirikae, Daiichi Pure Chemicals, Tokyo, Japan) origin.
Analysis of Lyn Phosphorylation.
Purified B cells (107 per group) were stimulated with lipid A (1 µg/ml) and harvested. Cells were solubilized in lysis buffer (1% Triton X-100, 150 mM NaCl, 50 mM Tris/HCl, pH7.5, 10 µg/ml soybean trypsin inhibitor, 1 mM PMSF, and 5 mM EDTA) supplemented with phosphatase inhibitors (10 mM NaF and 1 mM sodium vanadate). Lysates were applied to protein G Sepharose conjugated with anti-Lyn mAb Lyn8 (Wako Pure Chemicals). Precipitates (2.5 x 106 cells per lane) were subjected to SDS-PAGE (9% acrylamide) and electroblotting. Phosphotyrosine or Lyn was probed with antiphosphotyrosine mAb 4G10 (Upstate Biotechnology) or with Lyn8, respectively. Detection was conducted with goat antimouse IgG peroxidase and the SuperSignal chemiluminescence substrate (Pierce Chemical Co.).
Analysis of Humoral Immune Responses.
The littermate control and RP105-deficient mice were immunized intraperitoneally with 20 µg of TNP-LPS in PBS (three to five mice per group). The serum concentration of TNP-specific Abs at different time points was measured by ELISA. ELISA was performed by coating plastic plates with TNP-BSA (10 µg/ml), and serial serum dilutions were applied onto the plate. Bound Abs were revealed by goat Abs specific for IgM or IgG3 isotype (Caltag Labs.).
The analyses of serum concentrations of the Abs of different isotypes in mice immunized with T cellindependent and T celldependent antigens were done as described (18). In brief, wild-type, RP105+/-, and RP105-/- mice were immunized intraperitoneally with 5 µg of the T-independent antigen 4-hydroxy3-nitrophenylacetyl (NP)25-Ficoll in PBS or 50 µg of the alum-precipitated T-dependent antigen NP15chicken -globulin (CG). The serum concentration of NP-specific Abs was measured by ELISA.
Analysis of the Nuclear Factor B Activation in Ba/F3 Cells.
The IL-3dependent Ba/F3 cells (19) carrying the luciferase gene under the control of the nuclear factor (NF)-B responsive element (20) were used to study the role of TLR4 and RP105 in the LPS-induced NF-
B activation. The pEF-BOS expression vectors (21) encoding the RP105, TLR4, MD-1, or MD-2 proteins of human or mouse origin were constructed as described (9) (10) (12) (14) (15). These vectors, as well as the reporter gene, were transfected in the Ba/F3 cells as described previously (9) (10). Ba/F3 cells expressing TLR4MD-2, TLR4RP105, or TLR4RP105MD-1 were established by sequential transfection of those expressing TLR4 and the reporter gene. Cell surface expression of human TLR4 and TLR4MD-2 was shown previously (9). Expression of human RP105 and MD-1 was determined with the anti-RP105 mAb or the Ab against FLAG epitope (Sigma-Aldrich) that had been attached at the COOH terminus of MD-1, respectively. The expression levels of mouse RP105MD-1 and TLR4MD-2 were determined by FACS® using the mAbs RP404 and MTS510, which recognize RP105MD-1 and TLRMD2 complexes, respectively, but not RP105 or TLR4 alone ((10); our unpublished results). Expression of mouse TLR4 alone was determined by immunoblot analysis with the Abs to the FLAG epitope that had been attached at the COOH terminus of TLR4 (10). Transfected Ba/F3 cells were treated with lipid A at various concentrations for 4 h. The luciferase activity was measured in cell lysates as described (9).
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Results and Discussion |
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To test whether RP105 controls B cell responses to LPS, as well as to address the possible role of RP105 in B cell development and activation, we have generated mice homozygous for the null mutation of the RP105 gene. The exon 3 of the RP105 gene, encoding 575 of 661 amino acid residues of RP105 protein, was replaced by the neor gene in E14.1 ES cells, which were used to generate R105-/- mice (Fig 1, a and b). The targeted modification of the RP105 gene eliminates the region encoding most of the extracellular LRR domain, the transmembrane, and cytoplasmic domains. As expected, B cells derived from RP105-/- mice do not express RP105 on the cell surface (Fig 1 c), and because of the replacement of almost an entire RP105 gene by neor are unlikely to express potentially functional truncated RP105 protein.
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The flow cytometric analyses and absolute numbers of the bone marrow cells, splenocytes, lymph nodes, and peritoneal cells derived from RP105-deficient mice did not reveal changes in sizes of developing and mature B cell subpopulations compared with the control wild-type mice (Fig 2; Table 1). These data exclude the significant role of RP105 in B cell development.
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The responsiveness of RP105-deficient B cells to LPS was tested in vitro. Using the MTS510 mAb that recognizes TLR4 in association with MD-2, but not TLR4 alone, we found the expression levels of TLR4MD2 on wild-type and RP105-defcient cells to be >10 times lower than on the surface of peritoneal thioglycollateelicited macrophages (10). Furthermore, incubation of the wild-type and RP105-defcient B cells with LPS, anti-IgM, or anti-CD40 Abs did not upregulate surface expression of TLR4MD-2 (data not shown). Incubation of the purified splenic RP105-deficient B cells with different amounts of LPS derived from three different bacteria strains (E. coli 0111: B4, E. coli 055: B5, and S. minnesota Re595) as well as with synthetic lipid A results in B cell proliferation at levels significantly lower than of LPS-triggered wild-type control B cells (Fig 3 a). However, RP105-deficient B cells were not as impaired as TLR4-mutated C3H/HeJ B cells, which did not respond to LPS or lipid A (Fig 3 a). Reduced proliferative response of RP105-deficient B cells was due to the reduced number of cells entering the cell cycle within the first 24 h after the stimulation with LPS, and could not be overcome by costimulation of B cells with IL-4 (data not shown). In contrast to impaired proliferation in response to LPS, the proliferative responses of RP105-deficient B cells and control wild-type B cells to the Abs to IgM, CD40, or CD38 and IL-4 were similar (Fig 3 b; data not shown). These data indicate the specific role of RP105 in B cell responses to LPS.
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Lyn-deficient B cells are impaired in LPS-induced B cell proliferation, demonstrating an important role of Lyn in B cell signaling of LPS (22) (23). Our earlier studies revealed the involvement of the LynBtkprotein kinase C ß signaling chain in B cell activation by RP105 (16). It seems possible that RP105 has a role in LPS-induced tyrosine phosphorylation of Lyn. We examined tyrosine phosphorylation of Lyn after LPS stimulation in wild-type and RP105-deficient B cells (Fig 3 c). A slight but appreciable increase in phosphorylation was observed 1 h after LPS addition in wild-type B cells (Fig 3 c, lanes 13), whereas LPS-induced Lyn phosphorylation appeared to be weaker in RP105-deficient B cells (Fig 3 c, lanes 46).
The positive role of RP105 in B cell activation by LPS was further confirmed by the analysis of humoral immune responses in RP105-deficient mice immunized with TNP-LPS. The titers of TNP-specific IgM and IgG3 were significantly lower in the sera of the immunized RP105-deficient mice compared with control mice (Fig 4 a). However, immunization with T cellindependent antigen NP-Ficoll and T celldependent antigen NP-CG resulted in equally strong immune responses in both wild-type and RP105-deficient mice (Fig 4b and Fig c). Furthermore, similar titers of serum immunoglobulins of different isotypes in RP105-deficient and wild-type control mice (data not shown) suggest unaltered humoral responses of RP105-deficient B cells to environmental antigens present in the conventional animal facility.
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The involvement of RP105 in LPS signaling suggests the possible function of RP105 either as a TLR4-independent LPS receptor or as a coreceptor of TLR4. Considering unresponsiveness in C3H/HeJ mice (Fig 3 a), RP105MD-1 cannot substitute for the lack of TLR4. We showed previously that association of MD-2 greatly enhances LPS signaling via TLR4 (9) (10). However, the TLR4MD-2 complex is hardly detectable on the B cell surface with the MTS510 mAb that specifically reacts with the TLR4MD-2 complex (10). Moreover, the MTS510 mAb has little effect on LPS-dependent proliferation of B cells (our unpublished results). B cells may employ another molecule to regulate LPS signaling via TLR4. We hypothesized that the RP105MD-1 complex, instead of MD-2, helps TLR4 to recognize and signal LPS in B cells. The functional cooperation between TLR4 and RP105 was tested in murine Ba/F3 cells that express neither TLR4 nor RP105 and do not respond to LPS (9). The Ba/F3 cells carrying the stably integrated exogenous TLR4, RP105, MD-1, or MD-2 genes alone or in different combinations were used. The expression levels of TLR4 or RP105 in the presence or absence of coexpressed RP105MD-1 or TLR4, respectively, were similar as judged by immunoprecipitation or cell surface staining with the corresponding Abs ((10); data not shown). The LPS-induced activation of NF-B in these cells was tested by the analysis of the NF-
Binduced upregulation of the luciferase gene activity controlled by the NF-
B responsive element (20). The Ba/F3 cells expressing RP105MD-1 did not respond to LPS derived from S. minnesota Re595 or lipid A (Fig 5 a; data not shown). On the other hand, Ba/F3 cells expressing mouse TLR4 alone respond to LPS, albeit at levels significantly reduced compared with Ba/F3 cells expressing TLR4 and MD-2 (Fig 5 a; reference (10)). The RP105 influence on the LPS signaling via TLR4 was addressed by expression of RP105 with or without MD-1 and TLR4 in Ba/F3 cells. The LPS signaling via TLR4 alone was not affected by expression of RP105 alone, whereas it was significantly enhanced in the presence of RP105MD-1 (Fig 5 a). We also examined Ba/F3 cells expressing both TLR4MD-2 and RP105MD-1, the LPS responses of which were not different from Ba/F3 cells expressing TLR4MD-2 (Fig 5 b).
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Roles of human RP105MD-1 in the LPS signaling via human TLR4 were next addressed. Similar experiments were conducted with Ba/F3 cells expressing human TLR4 and RP105. The surface expression levels of TLR4 or RP105 in the presence or absence of coexpressed RP105MD-1 or TLR-4, respectively, were similar ((9); data not shown). In contrast to the Ba/F3 cells expressing murine TLR4, the Ba/F3 cells that express human TLR4 alone do not respond to lipid A (Fig 5 c; reference (9)). The reason for the observed species-specific differences in LPS signaling via murine and human TLR4 alone is unknown. Coexpression of RP105MD-1 conferred LPS responsiveness less efficiently than that of MD-2 (Fig 5 c). Treatment of Ba/F3 cells coexpressing human TLR4 and RP105MD-1 with the mAb directed against human RP105 inhibited LPS-induced NF-B activation as effectively as with anti-TLR4 mAb alone (Fig 5 d). Treatment of Ba/F3 cells coexpressing human TLR4 and RP105MD-1 with a combination of the anti-RP105 and anti-TLR4 mAbs did not result in further decrease in LPS-induced NF-
B activation (Fig 5 d), thus supporting the cooperative rather than independent signaling capacities of TLR4 and RP105MD-1.
RP105MD-1 is thus able to enhance LPS signaling via TLR4 as MD-2 does. Either MD-2 or RP105MD-1 is required for LPS recognition and signaling by TLR4. However, the TLR4MD-2 complex is hardly detectable on the mouse B cell surface (10), where RP105MD-1 is abundantly expressed. Taken together with the results with Ba/F3 transfectants, we would like to conclude that the RP105MD-1 complex, rather than MD-2, regulates LPS signaling via TLR4 on the B cell surface. The structural basis for the RP105MD-1 and TLR4 cooperation remains unclear. Immunoprecipitation of TLR4 or RP105 from lysates of splenic B cells or Ba/F3 cells coexpressing RP105MD-1 and TLR4 has not resulted in coprecipitation of TLR4 or RP105, respectively (data not shown). Immunoprecipitation in the presence of LPS did not make any difference.
Finally, the important role of RP105 in B cell activation by LPS makes it a possible target for pharmacological intervention aimed at enhancement of humoral immune response against Gram-negative bacteria.
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Footnotes |
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H. Ogata and I. Su contributed equally to this work.
1 Abbreviations used in this paper: CG, chicken -globulin; ES, embryonic stem; LRR, leucine-rich repeat; neor, neomycin resistance; NF, nuclear factor; TLR, Toll-like receptor.
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Acknowledgements |
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We thank Dr. Paul W. Kincade for his encouragement and support during the initial phase of the project. Drs. Yoshihiro Miura, Rintaro Shimazu, Kenji Fukudome, and Teruo Kirikae are also appreciated for their help and technical suggestions.
The study was supported in part by grants from Uehara Memorial Foundation and Monbusho (the Ministry of Education, Science, and Culture of Japan). This work was also supported by the Deutsche Forschungsgemeinschaft through SFB243 (to I. Su, K. Rajewsky, and A. Tarakhovsky) and by the Fonds der Chemie (A. Tarakhovsky). I. Mecklenbräuker is supported by a Graduiertenkolleg fellowship from the Deutsche Forschungsgemeinschaft.
Submitted: 22 February 2000
Revised: 1 May 2000
Accepted: 4 May 2000
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References |
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