By
From the * Ben May Institute for Cancer Research, Committee on Immunology, Department of
Pathology; § Department of Medicine, University of Chicago, Illinois 60637; and Protein Design
Laboratories, Mountain View, California 94043
Anti-CD3 monoclonal antibodies (mAbs) are potent immunosuppressive agents used in clinical
transplantation. However, the activation-related adverse side effects associated with these mAbs
have prompted the development of less toxic nonmitogenic anti-CD3 mAb therapies. At present,
the functional and biochemical consequences of T cell exposure to nonmitogenic anti-CD3 is
unclear. In this study, we have examined the early signaling events triggered by a nonmitogenic anti-CD3 mAb. Like the mitogenic anti-CD3 mAb, nonmitogenic anti-CD3 triggered changes
in the T cell receptor (TCR) complex, including chain tyrosine phosphorylation and ZAP-70
association. However, unlike the mitogenic anti-CD3 stimulation, nonmitogenic anti-CD3 was
ineffective at inducing the highly phosphorylated form of
(p23) and tyrosine phosphorylation
of the associated ZAP-70 tyrosine kinase. This proximal signaling deficiency correlated with
minimal phospholipase C
-1 phosphorylation and failure to mobilize detectable Ca2+. Not
only did biochemical signals delivered by nonmitogenic anti-CD3 resemble altered peptide
ligand signaling, but exposure of Th1 clones to nonmitogenic anti-CD3 also resulted in functional anergy. Finally, a bispecific anti-CD3 × anti-CD4 F(ab)
2 reconstituted early signal
transduction events and induced proliferation, suggesting that defective association of lck with
the TCR complex may underlie the observed signaling differences between the mitogenic and
nonmitogenic anti-CD3.
OKT3, a murine antibody specific for the CD3 The mechanism of immunosuppression by anti-CD3
mAbs is complex. Mitogenic anti-CD3 mAbs modulate the
TCR, induce apoptosis, and induce generalized long-term
T cell unresponsiveness (6). Similarly, treatment of mice
with the nonmitogenic anti-CD3 results in internalization
of the TCR complex and depletion of T cells from the circulation and peripheral lymphoid organs. However, in
contrast with the mitogenic antibodies, anti-CD3-IgG3 does not appear to induce global T cell unresponsiveness (5). Thus, the different anti-CD3 mAbs may suppress T cell responses
by distinct mechanisms. Treatment with anti-CD3-IgG3
alters expression of several T cell surface molecules; both
CD44 and Ly-6C are upregulated after exposure to the chimeric anti-CD3 (7). Thus, the interaction of anti-CD3-
IgG3 with T cells is not inert, but may deliver at least a
partial TCR signal that contributes to its immunosuppressive activity.
Currently, it is thought that TCR signaling results from
a cascade of events requiring the recruitment and activation
of nonreceptor tyrosine kinases. One of the earliest consequences of TCR engagement by mAb or peptide-MHC is
the tyrosine phosphorylation of components of the TCR
complex (8). The Until recently, it was thought that this cascade of events
was always fully engaged after exposure to peptide-MHC
ligand or mAbs and that different responses to stimuli reflected a quantitative addition of the number of receptors
engaged. However, antigenic peptide analogues, designated
as altered peptide ligands (APL), have illustrated that the
TCR is not an on-off switch. Rather, stimulation with
APL can result in qualitative differences in the early signals
transduced through the TCR. Specifically, stimulation with
APL results in a characteristic biochemical pattern involving partial In the present study, we examined the effect of nonmitogenic anti-CD3 mAbs on early signaling events in T cell
clones. The anti-CD3-IgG3 chimeric antibody transduced
a signal characterized by an altered pattern of Animals.
6- to 8-wk-old BALB/c, DBA/2J, and B10.A mice
were purchased from Frederick Cancer Research Institute Laboratories (Frederick, MD). DO 11.10 mice, transgenic for an OVA
peptide (323-339)-specific, I-Ad-restricted Cell Lines, Antibodies, and Reagents.
The chicken OVA-specific Th1 clone pGL10 was provided by Dr. F. Fitch (University of
Chicago, Chicago, IL) and the pigeon cytochrome c (PCC)-specific
Th1 clone, AE.7, was provided by Dr. M. Jenkins (University of
Minnesota, Minneapolis, MN). OVA and PCC were purchased
from Sigma Chem. Co. (St. Louis, MO). The following mAbs were
used in this study: 145-2C11 (anti-CD3), AT83A (anti-Thy-1) (provided by Dr. F. Fitch, University of Chicago, Chicago, IL); anti-CD3-IgG3 (5, 7); the anti-Ig antisera: goat anti-mouse IgG3
(Sigma), rabbit anti-mouse IgG3 (Zymed, San Francisco, CA), rabbit anti-hamster (Cappel, Durham, NC); 145-2C11-FITC
(Boehringer Mannheim, Indianapolis, Indiana); PV-1 (anti-CD28)
(Dr. C. June, Naval Medical Research Institute, MD); H146
(anti- Anti-CD3-Fos × Anti-CD4-Jun Bispecific F(ab Proliferation Assays.
pGL10 and AE.7 T cell clones were maintained as previously described (19, 20). T cell clones were purified
by Ficoll-HyPaque density centrifugation before use in all experiments. BALB/c spleens were lysed in hypotonic ACK buffer to
remove erythrocytes. Proliferation and unresponsiveness assays
were in 5 or 10% FCS supplemented DMEM. In a 96-well flat-bottomed plate, 2 × 105 splenocytes or 1 × 105 pGL10 T cells were
incubated on ice for 10 min with anti-CD3 (final concentration
of 1 µg/ml), and then for another 10 min on ice with the appropriate cross-linker (rabbit anti-IgG3 at 1:30 or goat anti-IgG3 at
1:100 or goat anti-hamster at 1:300), before being placed at 37°C.
For anti-CD3-Fos homodimer or anti-CD3 × anti-CD4 heterodimer proliferation assays, antibodies were serially diluted in
96-well flat-bottomed plates, starting at 10 µg/ml. Assays were
pulsed with 1 µCi/well of [3H]thymidine for the last 8 h of a 48-h
incubation, harvested on a Filtermate 196 96-well plate harvester
(Packard Instrument Co., Meriden, CT), and counted on a Packard TopCount microplate scintillation counter. Results are presented as the mean of triplicate cultures. Standard errors were less
than 20% of the mean.
chain
of the TCR complex, has been used clinically for
over a decade in the treatment of steroid-resistant graft rejection (1). However, use of this antibody has been hampered by a toxic first dose reaction syndrome shown to be
related to the initial T cell activation events and ensuing release of cytokines before the suppression of T cell responses
(2, 3). Others have demonstrated that the mitogenic activity of OKT3 and other anti-CD3 mAbs depends upon extensive TCR-CD3 cross-linking via binding to FcR-positive cells (4). Therefore, recent efforts have been devoted
to developing nonmitogenic forms of anti-CD3 by altering
binding to Fc receptor. As a model system, an anti-murine
CD3 mAb, 145-2C11, was genetically altered to eliminate
FcR binding: its variable region gene was fused to a murine
IgG3 Fc region, a mouse isotype with low affinity for murine FcR. This chimeric anti-CD3-IgG3 antibody has
been shown to be nonmitogenic in vitro, and did not result
in the serum cytokine elevation observed with the whole
145-2C11 mAb in vivo (5). However, the administration
of nonmitogenic anti-CD3 mAbs was equally effective in
prolonging graft survival as the parental 145-2C11 antibody (5). As similar non-FcR-binding mAbs derived from
OKT3 are being tested clinically, it is important to gain further understanding of the mechanism(s) by which these
nonmitogenic mAbs suppress T cell responses.
chain of the TCR complex contains three immunoreceptor tyrosine-based activation motifs (D/
EXXYXXL(X)6-8 YXXL) that become variably phosphorylated after TCR-CD3 ligation (9). It is thought that the
activation-induced 21- and 23-kD phosphorylated bands
evident on one-dimensional SDS-PAGE represent differentially phosphorylated forms of
(10). The other CD3 chains,
,
, and
(containing one immunoreceptor tyrosinebased activation motif each), become tyrosine phosphorylated as well (8). It has been hypothesized that the src family kinases, lck or fyn, may be responsible for these early
phosphorylation events (9). Within minutes, additional tyrosine phosphorylated proteins, including the ZAP-70
kinase, associate with the TCR-CD3 complex (11). These
proximal events lead to a series of biochemical signals that
activate downstream substrates in the PI-3 kinase, Ras, and
phospholipase C
-1 (PLC
-1)1 pathways, ultimately leading to activation of the T cell (9).
phosphorylation and ZAP-70 association in
the absence of phosphorylation, ultimately leading to a lack
of inositol-trisphosphate (IP3) turnover (10, 12, 13). The
delivery of such a partial signal effectively shuts down T cell
clones, resulting in the induction of unresponsiveness as
manifested by an inability of the anergized T cell clones to
produce IL-2 when rechallenged under optimal conditions.
chain and
ZAP-70 tyrosine phosphorylation, leading to defects in
downstream signaling events. Nonmitogenic anti-CD3 also
failed to induce higher order aggregation of the TCR into
a cap. Similar signaling changes were observed in naive T
cells at the level of TCR complex and PLC
-1 tyrosine
phosphorylation. Specific defects in early signal transduction and mitogenicity were restored by coaggregating CD4
(and thus presumably lck) with the TCR-CD3 complex. The
nonmitogenic anti-CD3 induced T cell unresponsiveness
in Th1 clones but not naive T cells. Together, these results
suggest that administration of nonmitogenic anti-CD3 mAbs,
especially in recipients undergoing acute transplant rejection, may result in the delivery of a partial T cell signal that
renders activated T cells unresponsive.
-TCR, were a gift
of Drs. D. Loh and K. Murphy (Washington University, St.
Louis, MO) (14). All mice were bred and maintained in a specific
pathogen-free facility at the University of Chicago.
mAb containing supernatant) (Dr. F. Fitch); 4G10 (antiphosphotyrosine) and anti-PLC
-1 (mixed mAbs) (UBI, Lake
Placid, NY); 12-222 (anti-ZAP-70 antiserum) (Dr. A. Weiss,
University of California, San Francisco, CA). Cyclosporine A was
purchased from Sandoz (Basel, Switzerland). Indo-1 was purchased from Molecular Probes (Eugene, OR).
-Zipper)2 Production.
The anti-CD3 antibody was derived from hamster anti-
mouse CD3 hybridoma 145-2C11 (15), and the anti-CD4 antibody from rat anti-mouse CD4 hybridoma GK1.5 (16). The VH
and VL sequences for 145-2C11 (5), (GenBank accession no.
U17870 and U17871) and GK1.5 (16, 16a) had been determined.
Homodimers of anti-CD3-Fos and anti-CD4-Jun F(ab
-zipper)2
were expressed by the genetic method described by Kostelny et
al., (17). Anti-CD3-Fos and anti-CD4-Jun were individually purified from transfected Sp2/0 spent media by protein G-Sepharose
affinity chromatography (18). The two homodimers were then reduced and reoxidized to form bispecific F(ab
-zipper)2 as described (17). Bispecific F(ab
-zipper)2 was further purified by
BAKERBOND Abx column chromatography or hydrophobic interaction chromatography on a Bio-Gel® Phenyl-5 PW column.
Biochemistry.
T cell clones or BALB/c lymph node cells were
washed in PBS and then resuspended in ice-cold PBS at 1 × 108/ml
or 2 × 108/ml, respectively. Anti-CD3-IgG3 was added at 4-5
µg/ml for a 10-min incubation on ice. An equal volume of antiIg cross-linker or PBS prewarmed to 37°C was added and samples
were incubated a further 2.5-5 min in a 37°C water bath. For
anti-CD3-Fos homodimer and anti-CD3 × anti-CD4 heterodimer
stimulations, cells were stimulated with 10 µg/ml of antibody. After the incubation, an equal volume of ice-cold 2× lysis buffer
was added (final concentration: 0.5% Triton X-100, 50 mM Tris
[pH7.6], 100 mM NaCl, 5 mM EDTA, 1 mM Na3VO4, 10 µg/
ml each leupeptin and aprotonin, 25 µM NPGB, and 1 mM
PMSF). For immunoprecipitations, 20 of a 50% protein A-agarose bead slurry (Pharmacia-UpJohn, Uppsala, Sweden) were
coated with 200
of mAb-containing supernatant or 2
of antisera for 1 h at 4°C. Lysates were added to the precoated protein
A-agarose beads and incubated 1 h at 4°C. The samples were resolved on a 12% SDS-polyacrylamide gel for
immunoprecipitations or an 8% gel for PLC
-1, and then transferred to PVDF
membrane (Millipore, Bedford, MA). Blots were blocked with
10% BSA (Sigma, St. Louis, MO). After incubation with primary
antibody and the appropriate horseradish-peroxidase-coupled secondary antibody, blots were developed by Enhanced Chemiluminescence (Amersham Corp., Arlington Heights, IL). Densitometry
measurements were performed using an AMBIS Image Acquisition and Analysis instrument (San Diego, CA).
Calcium Flux. pGL10 were washed with DMEM containing 10 mM Hepes at pH 7.0 and then incubated at 5 × 106 cells/ml with 5 µM indo-1 at 37°C for 30 min. An equal volume of DMEM with Hepes and 5% FCS at pH 7.4 was added and cells were incubated 30 min. Cells were washed twice with 5% FCS-supplemented DMEM at pH 7.2 and resuspended at 1 × 106/ml for data acquisition on a FACStar® plus (Becton Dickenson Immunocytometry Systems, Mountain View, CA). Cells were briefly warmed before stimulation with anti-CD3-IgG3 (1 µg/ml) plus rabbit anti-IgG3 (1:30). An increase in the 404:495 nm indo-1 emission ratio is indicative of a rise in intracellular Ca2+. Results were analyzed using Multitime (Phoenix Flow Systems, San Diego, CA).
Confocal Microscopy.
Purified DO 11.10 lymph node T cells
or pGL10 cells (106) were incubated with 5 µg/ml 145-2C11-
FITC on ice for 10 min and then stimulated with an equal volume of 37°C prewarmed goat anti-hamster in PBS (1:300 final)
for 0 or 5 min at 37°C. T cells were transferred to a polystyrene
FACS® tube and fixed in 3% paraformaldehyde for 10 min at
room temperature, washed three times with PBS, and then resuspended in 25 of mounting solution (0.5 mg/ml O-Phenylenediamine, 90% glycerol, 0.05 M Tris [pH 8.0], 0.2% NaN3). Samples were analyzed on a ZEISS 410 confocal microscope.
Unlike the original 145-2C11 mAb, the anti-CD3-IgG3 chimeric antibody does not induce proliferation or IL-2 production in whole spleen cells (5). Also, soluble 145-2C11 failed to induce proliferation of T cell clones in the absence of FcR-mediated cross-linking (Bluestone, J.A. and J.A. Smith, unpublished observations; data not shown). To test directly the role of multivalent crosslinking, a secondary IgG3-specific cross-linking antibody was added to cultures containing the anti-CD3-IgG3 mAb. The addition of the cross-linking reagent reconstituted a mitogenic stimulus for both fresh murine splenocytes and a T cell clone (Fig. 1). Thus, the induction of proliferation by anti-CD3 requires a higher order of TCR aggregation that cannot be achieved by bivalent Ab binding alone.
Nonmitogenic Anti-CD3 Renders T Cell Clones Hyporesponsive.
Although insufficient for induction of T cell proliferation or cytokine production, the anti-CD3-IgG3 mAb may deliver at least a partial signal, which alters T cell function. Therefore, the effects of anti-CD3-IgG3 on the functional responses of naive cells and Th1 clones were examined. pGL10 T cells or DO11.10 lymph node cells were cultured in the presence of splenic accessory cells (to compensate for the presence of non-T cells in the naive population) and nonmitogenic anti-CD3-IgG3. Previous studies have shown that treatment of T cells with anti-CD3-IgG3 resulted in downmodulation of TCR expression within 24 h (7). Therefore, after 24 h, the cells were washed, and recultured for 3 d to allow reexpression of the TCR (data not shown). As seen in Fig. 2 A, upon restimulation with the mitogenic 145-2C11 mAb plus splenic APCs, thymidine incorporation by anti-CD3-IgG3-treated pGL10 was markedly reduced as compared with pGL10 cultured with media alone. In contrast, the functional responses of murine lymph node T cells were not affected by culture with anti-CD3- IgG3. The clonal unresponsiveness did not merely reflect decreased viability, because anti-CD3-IgG3-treated clones proliferated in the presence of exogenously added IL-2 (data not shown). The effect of anti-CD3-IgG3 was not specific to the pGL10 clone, because the nonmitogenic anti-CD3 rendered the PCC-specific clone, AE.7, hyporesponsive as well (Fig. 2 B). To determine whether the reduced proliferation of anti-CD3-IgG3-treated T cell clones correlated with IL-2 production, pGL10 clones were cultured with or without anti-CD3-IgG3 for 24 h, rested, and then restimulated with immobilized anti-CD3 plus anti-CD28 (PV-1), conditions known to induce readily detectable IL-2 production (Fig. 2 C). Anti-CD3-IgG3-treated clones secreted significantly less IL-2 than the media-treated control cells. These data indicated that exposure to soluble, noncross-linked anti-CD3 selectively reduces the responsiveness of Th1 clones as compared with naive cells.
To examine whether the presence of CsA or CD28 costimulation would affect the nonmitogenic anti-CD3-induced unresponsiveness, pGL10 T cells were cultured with nonmitogenic anti-CD3 alone, or nonmitogenic anti-CD3 in the presence of CsA, or splenic APCs and anti-CD28 (Fig. 2 D). CsA partially blocked the induction of unresponsiveness by nonmitogenic anti-CD3, suggesting that this process may depend upon a calcium signal. In contrast, addition of anti-CD28 mAb in the primary culture failed to restore secondary responses.
Nonmitogenic Anti-CD3 Delivers a Partial TCR Signal.The functional consequences of culture with nonmitogenic
anti-CD3 support the hypothesis that anti-CD3-IgG3 delivers a signal. Therefore, experiments were performed to
determine the nature of the TCR signal triggered by nonmitogenic anti-CD3. Upon ligation of the TCR, one of
the earliest events to occur is the tyrosine phosphorylation
of components of the TCR complex ( and CD3
, CD3
,
and CD3
) (8). Phosphorylation of these chains allows subsequent association and phosphorylation of a variety of
other proteins, including the protein tyrosine kinase, ZAP70 (9). In the present study, the ability of nonmitogenic
anti-CD3 to trigger these crucial proximal events in T cell
clones was examined. T cells were stimulated with the
anti-CD3-IgG3 mAb in the presence or absence of a secondary Ig cross-linker. The TCR complex was immunoprecipitated with anti-
and analyzed for tyrosine phosphorylation (Fig. 3 A). Stimulating T cells with anti-CD3 under
cross-linking conditions induced both 21- and 23-kD forms
of phosphorylated
(p21 and p23) as well as phosphorylation of CD3
. The phosphorylated band below p21 (~18
kD) most likely represents another isoform of phosphorylated
(21). In contrast, the noncross-linked anti-CD3- IgG3 mAb induced similar levels of phosphorylated CD3
and p21
, but significantly less p23
. Quantitation of the
p21 and p23 bands by densitometry in multiple T cell
clone experiments (n = 4) revealed a consistent correlation
between the degree of anti-CD3 cross-linking and the
p23/p21 ratio; conditions that promote cross-linking increased the relative level of p23 expression (Fig. 3 D).
Examination of the phosphoproteins that coprecipitated
with the chain in darker exposures or greater cell number
revealed further differences between anti-Ig cross-linked
and noncross-linked conditions (Fig. 3 B). Unlike the crosslinked anti-CD3 stimulation, several of these phosphoproteins (bands between 30 and 46 kD as well as at 70 and 76 kD)
were missing or reduced in the anti-
precipitations from
T cells stimulated with the noncross-linked anti-CD3 mAb.
The proximal signals triggered by nonmitogenic anti-CD3 in lymph node T cells were similar to those induced in
clones in that (a) nonmitogenic anti-CD3 induces phosphorylation of TCR chains and (b) in the absence of crosslinking, several TCR-associated phosphotyrosine containing proteins are missing or reduced in intensity (Fig. 3 C).
Thus, in both T cell clones and naive T cells, the antiCD3-IgG3-induced TCR activation complex (with associated signaling molecules) contained fewer tyrosine phosphorylated molecules than a mitogenically stimulated TCR
complex. These results suggest that although nonmitogenic
anti-CD3 induces some tyrosine phosphorylation of
and
the CD3 chains, it is deficient in triggering other proximal signaling events.
Previous studies have shown that the 70-kD band observed in anti- precipitates represents the TCR-associated
tyrosine kinase, ZAP-70 (22). The reduced intensity of this
band in the immunoprecipitates from nonmitogenic antiCD3-treated cells could either represent a failure of ZAP70 association or deficient phosphorylation. To address this
issue, T cells were stimulated with cross-linked or noncross-linked anti-CD3, and the lysates were immunoprecipitated with anti-
. The Western blot was probed with
an anti-ZAP-70 antisera. As seen in Fig. 4, the noncrosslinked anti-CD3-IgG3 induced similar levels of ZAP-70
recruitment to the TCR complex; yet as confirmed by reprobing the blot with anti-phosphotyrosine, the proportion of ZAP-70 that was tyrosine phosphorylated was significantly reduced. Thus, in the absence of CD3 cross-linking,
ZAP-70 associates with the TCR-CD3 complex, but it is
not efficiently phosphorylated.
Defects in Downstream Events in the Absence of TCR-CD3 Cross-linking.
The differences in proximal signal transduction observed in the absence of cross-linking were likely to
be reflected in critical downstream biochemical events,
such as the tyrosine phosphorylation (and thus activation
of) PLC-1. To examine this event, pGL10 were stimulated with anti-CD3-IgG3 in the presence or absence of
cross-linker (Fig. 5 A). The dramatic increase in PLC
-1
phosphorylation observed in the presence of a secondary
cross-linking Ab was not observed following anti-CD3-
IgG3 stimulation alone. Similarly, cross-linking with antiIgG enhanced PLC
-1 tyrosine phosphorylation induced
by the nonmitogenic anti-CD3 in naive cells (Fig. 5 B).
Because anti-CD3-IgG3 was unable to induce significant PLC-1 phosphorylation, it was anticipated that one
of the events that depends upon PLC
-1 activation, Ca2+
mobilization, would likewise be impaired. T cell clones
were loaded with the calcium-sensitive dye indo-1 and then
analyzed by FACS® for calcium flux. A calcium flux was
not detected when the cells were stimulated with the antiCD3-IgG3 alone, even after 5 min. However, in T cells
incubated with anti-CD3-IgG3 followed by the addition
of a secondary cross-linker, a characteristic calcium flux was
observed within 1 min (Fig. 5 C). Anti-IgG Abs in the absence of anti-CD3 did not result in a calcium flux (data not shown). These results demonstrate that the downstream
signaling events of PLC
-1 activation and the ensuing
Ca2+ flux are dependent upon extensive cross-linking of
the TCR-CD3 by anti-CD3 mAbs.
Aggregation of the TCR complex has been shown to
correlate with T cell activation; Kupfer et al. (23) demonstrated that when T cells encounter antigen-MHC on
APCs, the TCR redistributes on the cell surface to form an
aggregated activation cap. This redistribution is a signalingdependent process most likely involving reorganization of
the cytoskeleton (24, 25). To test whether the addition of a
cross-linking Ab to anti-CD3 results in an aggregated TCR
cap, confocal microscopy was performed on pGL10 and
purified DO11.10 T cells incubated with anti-CD3 (2C11-
FITC) under cross-linking versus noncross-linking conditions (Fig. 6). In the presence of cross-linking Ab, antiCD3 stimulation induces aggregation of the TCR into a
cap on one side of the cell. However, in the absence of
cross-linker, the anti-CD3 remained diffusely distributed
on the cell surface. Thus, the signal delivered by nonmitogenic anti-CD3 appeared insufficient for the redistribution
of TCRs into an aggregated cap.
Recruitment of CD4-lck into the Complex Reconstitutes Complete Proximal Signal Transduction and Mitogenicity.
The
inability of nonmitogenic anti-CD3 to trigger specific
downstream events and proliferation most likely stems from
the defective proximal events observed involving and
ZAP- 70. Previous studies have suggested that the src family kinase, lck, plays a crucial role in the phosphorylation of
, which subsequently allows association and phosphorylation of ZAP-70 (26, 27). Thus, it was possible that the differences in
and ZAP-70 phosphorylation seen upon the
addition of cross-linker to anti-CD3 may have reflected increased lck activation or enhanced recruitment to the TCR.
Initial experiments examining lck activation by monitoring
lck tyrosine phosphorylation revealed no differences between cross-linking and noncross-linking conditions. It is
clear that CD4 associates with lck and can interact with the
TCR complex inducibly upon TCR ligation of antigen-
MHC. Thus, artificially bringing CD4-lck into the TCR
complex might reconstitute a mitogenic anti-CD3 stimulus, even in the absence of a secondary cross-linking Ab.
To test this hypothesis, we took advantage of a bispecific
anti-CD3 × anti-CD4 reagent prepared by a molecular approach to insure the presence of monovalent arms specific
for CD3 and CD4 (as described in Materials and Methods).
T cells were incubated with anti-CD3-Fos homodimer or
the anti-CD3 × anti-CD4 bispecific F(ab)
2, lysed, and the TCR-CD3 complex was then immunoprecipitated and
analyzed. The bispecific construct induced significant p23
, ZAP-70 phosphorylation, as well as association of the
phosphoproteins between 30-46 kD (Fig. 7 A), even in the
absence of a secondary cross-linking antibody. In contrast,
the overall pattern induced by anti-CD3-Fos resembled the results seen in T cells stimulated with the anti-CD3-
IgG3 mAb: specifically, a reduced association of phosphoproteins and barely detectable ZAP-70 phosphorylation. In
the lysates of T cells stimulated with the bispecific antiCD3 × anti-CD4 construct, a large tyrosine-phosphorylated
protein was observed that migrated just above the heavy
chain. This phosphoprotein is likely to be p56 lck based on
protein size. This band never appeared in the cross-linked
anti-CD3 studies. One possible explanation for this difference is that in the absence of CD4 coaggregation, lck may dissociate from the TCR complex after lck phosphorylates
its substrates. Whereas under stimulation conditions using
the bispecific antibody, lck remains in the complex longer
due to stable association with coaggregated CD4.
The biochemical results suggested that the anti-CD3 × anti-CD4 bispecific antibody delivered a competent activating signal to the T cells. In fact, T cell clones or fresh murine T cells cultured in the presence of anti-CD3 × anti-CD4 heterodimer proliferated, whereas T cells cultured with the anti-CD3-Fos homodimer did not (Fig. 7 B). Thus, enhanced association of lck with the TCR complex reconstituted both early signaling events and a mitogenic stimulus in the absence of further Ab cross-linking.
In this study, we have shown that bivalent anti-CD3
delivers a partial TCR signal that renders Th1 clones hyporesponsive. This signal consists of phosphorylation of several components of the TCR complex, (bands representing
CD3, CD3
), ZAP-70 association, and partial phosphorylation of TCR
; in the absence of cross-linking, there is a
relatively greater induction of the phosphorylated p21
as
compared with the p23
band species evident in T cell clones. Presently, it is unclear what the p21 and p23 forms
of
represent. p21 induction appears to be sufficient for
association of the ZAP-70 kinase with the TCR complex,
whereas p23 induction and ZAP-70 phosphorylation appear to be interrelated events. Indeed, the low level of
ZAP-70 phosphorylation observed in the noncross-linked situation correlates with the small amount of p23
that is
generated. In a recent study, Weist et al. (28) proposed that
the p23 form of
observed in thymocytes upon in vitro
stimulation depends on greater TCR aggregation. Our findings are consistent with this hypothesis. Higher orders of
TCR aggregation also appear to be required for recruitment of other phosphotyrosine containing molecules to the
TCR-CD3 complex in both T cell clones and bulk naive
cells. If any of these tyrosine-phosphorylated molecules contain SH2 domains, they may require the fully phosphorylated p23 form of
for association. Alternatively, the p23
form may be required for docking of a kinase that phosphorylates these associated molecules.
The observation that noncross-linked anti-CD3 induces
less ZAP-70 phosphorylation and p23 phospho- bears a
striking resemblance to the findings in the altered peptide
ligand studies (10, 13). The relative contribution of affinity
for MHC or TCR (and thus occupancy) versus TCR aggregation has been unclear in these systems. Recently, Lyons et al. (29) showed a correlation between antagonist activity of certain altered peptide ligands and a higher TCR
dissociation rate. However, this finding does not exclude a
role for aggregation in that a shorter dwell time of the
TCR may fail to induce the oligomerization required for a
fully activating stimulus. In the present study, the issue of
affinity has been addressed; the same primary antibody was
used in both cross-linked and noncross-linked situations;
thus, intrinsic affinity for TCR was held constant. Because
similar signaling deficits were found in noncross-linked anti-CD3 and altered peptide ligand stimulations, it is possible that the altered peptide ligands may induce their characteristic partial signals because of insufficient TCR aggregation.
There are several ways in which the localization of multiple TCR complexes within a large aggregate could enhance signaling. In the kinetic proofreading model proposed by McKeithan (30), TCR signal transduction was
modeled as a reversible multistep pathway containing sequential phosphorylation events. In this paradigm, aggregation of TCRs might enhance propagation of the signal by
favoring phosphorylation over dephosphorylation. On a
more mechanistic level, aggregation may aid in recruiting
key signaling molecules; recruitment of molecules may be
further stabilized if there are multiple potential contact points
(catalytic sites, SH2 domains, or other recognition motifs)
between components of the TCR complex that are in close
proximity. For example, if lck binds one phosphorylated
ZAP-70 through its SH2 domain, the lck would be in a
prime position to phosphorylate a neighboring ZAP-70 molecule in the TCR aggregate. In the noncross-linked
situation, lck might migrate away before phosphorylating
more ZAP-70 molecules. Thus, aggregated TCR signal
transduction may result in amplification of these signals, because one kinase may act on multiple substrates. This capacity for amplification would mean that proximal differences should become magnified as the signal is propagated.
As seen in the present study, a relative reduction in ZAP70 phosphorylation leads to a more dramatic deficiency in
PLC-1 phosphorylation and Ca2+ flux.
The redistribution of TCR complexes to one pole, within minutes upon addition of secondary cross-linker to antiCD3, is likely to reflect changes in the underlying cytoskeleton. Others have shown that TCR engagement can lead to redistribution of cytoskeletal elements such as talin, vinculin, and actin (9, 24). The cross-linking Ab might be providing sufficient TCR aggregation to trigger a threshold signal for cytoskeletal mobilization. Studies by Valetutti et al. (25) have suggested that the cytoskeleton also plays an active role in sustaining a TCR signal, because the addition of agents which disrupt the actin cytoskeleton (e.g., Cytocholasin D) can block the rise in intracellular Ca2+. The cross-linked anti-CD3 system may be useful for dissecting the role of the cytoskeleton in proximal signaling events.
Our experiments suggest that efficient recruitment of lck may be the pivotal event accomplished by aggregation. Lck has been shown to be important for proximal signaling in that absence of lck almost completely abrogates tyrosine phosphorylation events (9, 31). It is well established that coaggregating anti-CD3 and anti-CD4 antibodies or using anti-CD3/anti-CD4 heteroconjugate mAbs can result in enhanced tyrosine phosphorylation and calcium mobilization (32). Recently, it was shown that in circumstances in which lck is limiting, as in double-positive thymocytes, ZAP-70 phosphorylation requires coaggregation of TCR and CD4 (28). Furthermore, blockading CD4 (and presumably its associated lck molecules) with anti-CD4 mAbs can convert a partial agonist signal into an antagonist signal with its associated characteristic signaling deficits (33). Thus, impaired CD4 recruitment has been proposed as a mechanism for altered peptide/antagonist peptide signaling. The pivotal nature of lck recruitment is underscored by our finding that secondary antibody induced aggregation can be dispensed with, if lck is recruited by bringing CD4 into the complex artificially. Even in the absence of exogenous cross-linking, stimulation with a bivalent anti-CD3 × antiCD4 reagent reconstituted both the early signaling events of ZAP-70 phosphorylation and association of other phosphorylated proteins with the complex. In turn, these early events lead ultimately to a mitogenic stimulus.
The partial signals delivered by nonmitogenic anti-CD3 correlated with the induction of functional anergy as defined by an inability to proliferate due to poor IL-2 production. The striking similarity between the signals delivered by altered peptide ligands and nonmitogenic anti-CD3 are perhaps indicative of a common mechanism of anergy induction. How these partial signals translate into an off signal that shuts down T cell clonal responsiveness has yet to be determined. In the classical model of anergy, involving a complete signal one (through the TCR) in the absence of signal two (costimulation), induction of unresponsiveness depends upon a successful calcium signal that can be blocked by CsA (34, 35). Similarly, CsA has been shown to block anergy induction by altered peptide ligands (12). In fact, an altered peptide ligand triggered calcium signal has been recently demonstrated using an exquisitely sensitive system (36). The ability of CsA to block nonmitogenic anti-CD3-induced functional anergy suggests that a calcium signal may be important in this process. It is possible that the lack of detectable calcium flux by nonmitogenic anti-CD3 reflects insufficient sensitivity. In contrast with the classical models of anergy, the presence of competent APC or anti-CD28 antibodies did not rescue T cell clones from nonmitogenic anti-CD3-induced unresponsiveness.
An unresolved observation was the finding that culture
with the nonmitogenic anti-CD3 suppresses IL-2 production in clones, but it did not appear to impair significantly
the responsiveness of bulk T cells. Our results suggest similar defects in signaling between naive cells and clones in
terms of ZAP-70 phosphorylation and TCR-CD3 complex-associated phosphorylated molecules, as well as the
downstream events of PLC-1 phosphorylation and TCR
capping. It is possible that naive cells and clones differ in
the way they respond to nonmitogenic anti-CD3 mAbs,
either in the triggering of other biochemical signals, or the
integration of downstream nuclear signals.
The mitogenic forms of anti-CD3 currently in use severely suppress global T cell responses. This study suggests that nonmitogenic anti-CD3 may selectively induce unresponsiveness in activated T cell subsets. These findings bear promising implications for transplant therapy in that it would be beneficial to be able to suppress the alloreactive T cells that mediate graft rejection, while maintaining the responsiveness of other T cells. Further investigation into the mechanisms by which nonmitogenic anti-CD3 modulates T cell responses should shed some light on the applicability of this potential therapeutic to transplantation.
Address correspondence to Dr. Jeffrey A. Bluestone, Committee on Immunology, 5841 S. Maryland Avenue, MC 1089, Chicago, IL 60637.
Received for publication 24 October 1996 and in revised form 4 February 1997.
1Abbreviations used in this paper: APL, altered peptide ligands; IP3, inositol- triphosphate; PCC, pigeon cytochrome C; PLCWe would like to thank J. Auger for her assistance with the calcium flux experiments and B. Eisfelder for his assistance with confocal microscopy.
This work was supported by PO1AI29531-07, CA-14599, HL07605, and a grant from the Baxter Foundation.
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