© Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 199, Number 4, 525-533
Activation of Natural Killer Cells and Dendritic Cells upon Recognition of a Novel CD99-like Ligand by Paired Immunoglobulin-like Type 2 Receptor
Ikuo Shiratori1,2,
Kouetsu Ogasawara3,
Takashi Saito1,4,
Lewis L. Lanier3, and
Hisashi Arase1,2
1 Department of Molecular Genetics, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan
2 Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Saitama 332-0012, Japan
3 Department of Microbiology and Immunology and the Cancer Research Institute, University of California, San Francisco, CA 94143
4 Laboratory of Cell Signaling, Institute of Physical and Chemical Research, Reseach Center for Allergy and Immunology, Yokohama 230-0045, Japan
Address correspondence to Hisashi Arase, Dept. of Molecular Genetics, Chiba University Graduate School of Medicine, 1-8-1 Inohana, Chuoku, Chiba 260-8670, Japan. Phone: 81-43-226-2198; Fax: 81-43-222-1791; email: arase{at}faculty.chiba-u.jp
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Abstract
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Paired receptors that consist of highly related activating and inhibitory receptors are widely involved in the regulation of the immune system. Here, we report a mouse orthologue of the human activating paired immunoglobulin-like type 2 receptor (PILR) ß, which was cloned from a cDNA library of natural killer (NK) cells based on its ability to associate with the DAP12 signaling adaptor protein. The activating PILRß was expressed not only on NK cells but also on dendritic cells and macrophages. Furthermore, we have identified a novel CD99-like molecule as a ligand for the activating PILRß and inhibitory PILR
receptors. Transcripts of PILR ligand are present in many tissues, including some T cell lines. Cells expressing the PILR ligand specifically activated NK cells and dendritic cells that express the activating PILRß. Our findings reveal a new regulatory mechanism of innate immunity by PILR and its CD99-like ligand.
Key Words: natural killer cell receptor tumor antigen innate immunity immunoglobulin superfamily DAP12
T. Saito and L.L. Lanier contributed equally to this work.
The present address of H. Arase is the Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
Abbreviations used in this paper: BM-DC, BM-derived DC; GFP, green fluorescent protein; IRES, internal ribosome entry site; ITAM, immunoreceptor tyrosine-based activation motif; ITIM, immunoreceptor tyrosine-based inhibitory motif; KIR, killer cell Ig-like receptor; MCMV, mouse cytomegalovirus; PILR, paired Ig-like type 2 receptor, PILR-L, PILR ligand.
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Introduction
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Activation of NK cells is controlled by both inhibitory and activating receptors (14). Human and mouse NK cells express inhibitory receptors that recognize MHC class I to avoid attack against normal cells. Inhibitory receptors possess immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains and deliver inhibitory signals via tyrosine phosphatases, such as SHP-1 (5, 6). On the other hand, NK cells may also express activating receptors that are highly homologous in their extracellular domains to the inhibitory receptors. Generally, genes for these paired inhibitory and activating NK receptors are located very closely in the genome and likely evolved by gene duplication (7). The activating receptors do not contain ITIM sequences in their cytoplasmic domains, and they exhibit a positively charged residue in transmembrane region that permits association with immunoreceptor tyrosine-based activation motif (ITAM)bearing adaptor molecules, such as DAP12 (1). Unlike inhibitory receptors that recognize MHC class I, activating receptors generally do not recognize MHC class I, and physiological roles of most activating receptors have remained unclear. Receptor pairs that consist of highly related inhibitory and activating receptors are not restricted to NK receptors. For example, families of receptors, such as leukocyte Ig-like receptor (also called CD85 or Ig-like transcript), paired Ig-like receptor (PIR), and signal-regulatory protein, are expressed on macrophages and DCs and consist of inhibitory and activating receptors (8).
Recently, we have proposed that mouse cytomegalovirus (MCMV) has acquired the m157 gene to encode a ligand for the inhibitory NK receptors to escape attack by NK cells (9). On the other hand, some MCMV-resistant mouse strains express activating NK receptors for m157 (9, 10). These findings suggested that NK cells may have evolved activating receptors from inhibitory receptors to control virus infection (11). This may have been accomplished by gene conversion in a process that used exons encoding the extracellular domain of an inhibitory receptor and an exon encoding the transmembrane of another activating receptor that allows for association with the DAP12 signaling adaptor (12). In addition, some activating human NK receptors, such as killer cell Ig-like receptor (KIR), exhibit a potential ITIM sequence in the 3' untranslated region (13). From these observations, we have proposed a new hypothesis that activating receptors have evolved from inhibitory receptors (13). This hypothesis may explain why activating receptors corresponding to inhibitory receptors exist in the immune system. ITAM-bearing adaptor proteins, such as DAP12, FcR
, and CD3
, are used for signal transduction by these paired activating receptors. Therefore, identification of receptors that associate with these adaptor molecules may be important to elucidate how NK cells recognize specific target cells, such as virus-infected cells.
An interesting characteristic of these ITAM-bearing adaptor molecules is their inability to be expressed stably on the cell surface in the absence of their partner receptor (14). By taking advantage of this property of the adaptor molecules and using retrovirus cDNA libraries, we have developed a sensitive screening system for receptors that associate with them. Using this system in the present work, we have identified a mouse orthologue of the human activating paired Ig-like type 2 receptor (PILR) ß (15). The PILR family consists of PILR
(also called FDF03) and PILRß (15, 16). Human PILR
contains an ITIM in its cytoplasmic domain and delivers inhibitory signals (15, 16). On the other hand, human PILRß does not contain an ITIM in its cytoplasmic domain but possesses positively charged amino acids in the transmembrane region (15). Therefore, PILRß has been suggested to be an activating receptor that associates with certain ITAM-bearing adaptor molecules. However, the physiological role, as well as ligands of PILR, have not been identified previously. In this paper, we demonstrate that mouse PILR recognizes a unique CD99-like protein expressed on several cell types, including T cells. Because the PILR is expressed not only on NK cells but also on macrophages, DCs, and granulocytes, our findings provide a new mechanism for the regulation of innate immunity.
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Materials and Methods
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Mice.
C57BL/6 mice were purchased from Japan SLC. DAP12-deficient mice backcrossed to C57BL/6 for nine generations were maintained in the University of California San Francisco animal facility (17).
Expression Cloning of DAP12-associated Receptors.
A NH2-terminal FLAG-tagged DAP12 was transfected into the 2B4 T cell hybridoma by using the pMx-neo retrovirus vector. Thereafter, we selected a single cell clone that did not express FLAG-DAP12 on the cell surface, but did express high levels of FLAG-DAP12 upon transfection with the DAP12-associated Ly49D receptor. 2 x 107 FLAG-DAP12 2B4 T hybridoma cells were infected with a retrovirus cDNA library generated from IL-2expanded C57BL/6 NK cells (18) at a viral titer to achieve
30% infection. FLAG-expressing cells were isolated by using a MACS purification system (Miltenyi Biotech). After two rounds of enrichment of FLAG-positive cells by MACS, almost all of cells were stained by the anti-FLAG mAb M2 (Sigma-Aldrich) and approximately half of them were stained by mixture of anti-Ly49D (4E5; BD Biosciences) and Ly49H mAbs (provided by W.M. Yokoyama, Washington University School of Medicine, St. Louis, MO). Cells that did not express Ly49D and Ly49H were purified by MACS. Single cell clones expressing FLAG-DAP12, but not Ly49D or Ly49H, were obtained by limiting dilution. The integrated cDNA was derived from the FLAG-positive cells by PCR using sense primer (5'-GGTGGACCATCCTCTAGACT-3') and antisense primer (5'-TTTATTTTATCGTCGATCGACC-3'), and the amplified genes were directly sequenced.
Functional Analyses for Adaptor Molecules Associated PILRs.
NH2-terminal FLAG-tagged PILR
or PILRß were transfected into MA5.8 T cells that lack CD3
, FcR
, and DAP12. ITAM-bearing adapters, including CD3
, FcR
, and DAP12, were cotransfected into the FLAG-tagged PILR transfectants by using the pMxinternal ribosome entry site (IRES)green fluorescent protein (GFP) retrovirus vector. The GFP-positive cells were sorted by using a FACSVantageTM (Becton Dickinson). The transfectants were stimulated with immobilized anti-FLAG mAb or control mAb. Culture supernatants were collected after 24 h, and amounts of IL-2 were measured by ELISA.
Generation of PILR
-Ig and PILRß-Ig and Staining with Ig Fusion Proteins.
cDNA fragments corresponding to the extracellular domains of PILR
and PILRß were amplified by PCR using sense primer (5'-AATCTCGAGGGAAATTCAGAAAGATCCAA-3') and antisense primer (5'-AATCTCGAGTGCCAACCCAACTGTGGTTT-3'), and sense primer (5'-AATCTCGAGGGAAATTCAGAAAGATACAACA-3') and antisense primer (5'-AATCTCGAGCAGGTTCATCAGTGAAGGAT-3'), respectively. The cDNA for mouse PILR
was inserted into the XhoI cloning site of a modified pME18S expression vector that contained a mouse CD150 leader segment at the 5' terminus, and the XhoI cloning site and the Fc segment of human IgG1 at the 3' terminus. The cDNA-encoding mouse PILRß was inserted into the XhoI cloning site of this expression vector, except that an additional IgA tail piece sequence was inserted at the 3' end to generate a dodecameric Ig fusion protein (19). COS7 cells were transiently transfected with the plasmids, and culture supernatants were collected using standard methods. For the staining of PILR ligand (PILR-L), cell lines were mixed with saturating concentrations of these fusion proteins for 30 min on ice, followed by PE-conjugated goat antihuman IgG (Jackson ImmunoResearch Laboratories) for 30 min. Stained cells were analyzed by using a FACSCaliburTM (Becton Dickinson).
Retrovirus cDNA Library Construction.
A retrovirus cDNA library was constructed as reported previously (18). In brief, cDNA was generated from mRNA purified from the EL-4 cell line using the Superscript plasmid system (Invitrogen) and was cloned into SalI and NotI sites of the pMxs retrovirus vector (18). Ligated cDNA was transformed into ElectroMAX competent cells (Invitrogen), and plasmids were purified by using a MAXI prep plasmid purification kit (QIAGEN).
Cloning of PILR-L and Generation of PILR-L Transfectants.
2 x 107 Ba/F3 cells were infected with a retrovirus cDNA library from EL-4 at an efficiency of
30%. 2 d after infection, cells were stained with PILRß-Ig and PE-conjugated goat antihuman IgG, and stained cells were purified by using a FACSVantageTM. After expansion of the purified cells, the cells were stained with PILRß-Ig plus PE-conjugated antihuman IgG and FITC-conjugated anti-Fc receptor mAb (2.4G2; BD Biosciences), and cells stained with PILRß-Ig, but not 2.4G2 mAb, were obtained. Single cell clones that were stained by PILRß-Ig were isolated by using a Clonocyte (Becton Dickinson). Library-derived genes were amplified by using the PCR GC Advantage system (CLONTECH Laboratories, Inc.) using sense primer (5'-GGTGGACCATCCTCTAGACT-3') and antisense primer (5'-TTTATTTTATCGTCGATCGACC-3'). Amplified cDNA was cloned into the pMxs retrovirus vector, and nucleic acid sequences were determined. PILR-L cDNA was subcloned into the pME18S expression vector and pMx-IRES-GFP retrovirus expression vector and transfected into 293T cells and Ba/F3 cells, respectively.
Surface Biotinylation and Immunoprecipitation.
Biotinylated Ba/F3 cells transfected with PILR-L were lysed with a lysis buffer containing 1% digitonin, 50 mM Tris-HCl (pH 7.6), 150 mM NaCl, 10 µg/ml aprotinin, 10 µg/ml leupeptin, 1 mM PMSF, and 10 mM iodoacetamide at a concentration of 107 cells/ml. Cell lysates were immunoprecipitated with protein A preincubated with PILR
-Ig, PILRß-Ig, and control Ig fusion protein, separated on SDS-PAGE gels, and transferred onto a polyvinylidene difluoride membrane (Immobilon-P; Millipore). The biotinylated proteins were detected by using streptavidin peroxidase (VECSTAIN Elite ABC kit; Vector Laboratories), the enhanced chemiluminescence detection system (Amersham Biosciences), and LAS1000 (Fujifilm).
Real-time Quantitative PCR.
cDNA from various tissues and purified cell populations were prepared as described previously (20). In brief, splenic B and T cells were stained with FITC-conjugated anti-B220 mAb (RA36B2; eBioscience) and PE-conjugated anti-CD3 mAb (1452C11; BD Biosciences), respectively, and the stained cells were purified by using a MACS. Peritoneal macrophages and granulocytes were elicited by the i.p. injection of 3% sodium casein, and peritoneal cells were harvested after 1 d. Peritoneal exudates were separated with 90% Percoll gradient centrifugation (60,000 g, 20 min) (Amersham Pharmacia). Mac-1+Gr-1- and Mac-1-Gr-1+ cells were purified by using a FACSVantageTM and were used as macrophages and granulocytes, respectively. BM cells were cultured in the presence of 20 ng/ml of mouse IL-3 for 4 wk and used as BM-derived mast cells. Splenic NK cells and BM-derived DCs (BM-DCs) were prepared as described in the next two paragraphs. Quantitative PCR was performed by using a SYBER green PCR kit (QIAGEN) and analyzed by an iCycler (Bio-Rad Laboratories). Primers used for amplification were as follows: ß-actin, sense primer (5'-TCTACAATGAGCTGCGTGTG-3') and antisense primer (5'-GGTACGACCAGAGGCATACA-3'); PILR
, sense primer (5'-TGGGGTTTTGGGATTGATAG-3') and antisense primer (5'-GCTGTTCCTGGTGAGGTCGG-3'); PILRß, sense primer (5'-CCCAGTACTTTAGTCGAGTTAA-3') and antisense primer (5'-TGAAGGATTCCTCTGGTCG-3'); and PILR-L, sense primer (5'-GGAGACTCAGGCGGCATCT-3') and antisense primer (5'-GATAGGCCACGAAGCTCGAC-3'). Transcription levels of PILR
, PILRß, and PILR-L were normalized to the amount of ß-actin. The relative amount of transcripts in various organs was compared with the spleen by dividing the transcription level of each tissue by that of spleen.
NK Cell Cytotoxicity Assays.
NKL human NK cell lines were transfected with pMx-IRES-GFP and pMx-IRES-GFP containing mouse PILRß, and GFP-positive cells were collected by using a FACSVantageTM. Mouse NK cells were purified as described previously (21). In brief, sIg-, CD4-, and CD8- splenocytes from C57BL/6 mice were stained with PE-conjugated DX5 mAb (eBiosocience), and DX5+ cells were obtained by using a MACS purification system. DX5+ NK cells were cultured in the presence of 1,000 U/ml IL-2 for 7 d. Cytotoxicity of NK cells against PILR-Ltransfected Ba/F3 cells and 293T cells were analyzed by 51Cr release assays using standard techniques.
Measurement of TNF
and NO Production.
BM-DCs were generated by culturing 5 x 106 BM cells with RPMI 1640 medium containing 10% FCS and 200 U/ml mouse GM-CSF for 78 d in six-well culture plates. Nonadherent cells were collected and cultured for 1 d. More than 95% of the cells expressed CD11b and CD11c. 5 x 105 BM-DCs were cocultured with various numbers of parental or PILR-Ltransfected 293T cells in 48-well plates, as indicated in Fig. 8. After 24 h, culture supernatants were collected, and amounts of NO2 or TNF
were measured by using the Griess reagent (Sigma-Aldrich) or a TNF
ELISA kit (Genzyme), respectively.
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Results
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Cloning of Mouse Activating PILRß.
Most activating NK receptors associate with ITAM-bearing signal transducing adaptor molecules such as DAP12, FcR
, and CD3
(2123). Cell surface expression of these adaptor molecules is very low in the absence of the receptors that associate with them. To identify activating NK receptors that associate with DAP12, we generated a T cell hybridoma expressing a NH2-terminal FLAG-tagged DAP12 (FLAG-DAP12) and isolated a single cell clone that lacks cell surface expression of FLAG-DAP12. We infected these cells with a retrovirus cDNA library generated from mouse NK cells (18) and selected and cloned cells expressing FLAG-DAP12 on the cell surface. Using this screening system, we cloned a mouse orthologue of the human activating PILRß (15), as well as Ly49D and Ly49H that were already known to associate with DAP12 (24). The amino acid homology between mouse PILRß and human PILRß was 38% (15). When mouse PILRß was transfected into the T cell hybridoma expressing FLAG-DAP12, cell surface expression of FLAG-DAP12 was induced by PILRß (unpublished data). This suggested that DAP12 associates with mouse PILRß.
Human and mouse PILR
(also called FDF03; references 15, 16) have been identified as an ITIM-containing inhibitory receptor that is highly related to PILRß. Fig. 1 demonstrates the amino acid comparison between mouse PILR
and mouse PILRß. PILRß, but not PILR
, contains a positively charged amino acid in the transmembrane region that is required for association with DAP12 (25). Amino acid homology of the extracellular domain between mouse PILR
and PILRß is 83%, with the NH2 terminus of the extracellular domain showing very high similarity. This suggested that PILR
and PILRß might recognize a common ligand.
Up-regulation of Cell Surface Expression of PILRß by DAP12.
We analyzed the role of ITAM-bearing adaptor proteins, including CD3
, FcR
, and DAP12, on the cell surface expression and signal transduction of mouse PILRß. NH2-terminal FLAG-tagged PILR
(FLAG-PILR
) or PILRß (FLAG-PILRß) was transfected into a T cell hybridoma, MA5.8, that lacks CD3
, FcR
, and DAP12. As shown in Fig. 2 A, FLAG-PILR
and FLAG-PILRß were expressed on the cell surface of the MA5.8 transfectants, as demonstrated by staining with an anti-FLAG mAb. Of note, FLAG-PILR
was expressed at a higher level on MA5.8 than PILRß. Next, we cotransfected CD3
, FcR
, or DAP12 into M5.8 cells expressing FLAG-PILRß (Fig. 2 B). The expression level of FLAG-PILRß was substantially increased when DAP12, but not CD3
or FcR
, was cotransfected into the MA5.8 cells bearing FLAG-PILRß. In contrast, DAP10 did not substantially up-regulate the expression of FLAG-PILRß (unpublished data). This suggests that DAP12 associates with PILRß and stabilizes the cell surface expression of PILRß.

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Figure 2. (A) Role of DAP12 in the cell surface expression and signal transduction of PILRß, FLAG-PILRß (solid line), and FLAG-PILR (dashed line) was transfected into the MA5.8 cell line. Parental MA5.8 (dotted line) and the transfectants were stained with anti-FLAG mAb. Mean fluorescence intensities of anti-FLAG mAb staining for parental MA5.8, FLAG-PILR , and FLAG-PILRßtransfected cells were 2.93, 27.0, and 369.1, respectively. (B) DAP12, CD3 , or FcR were transfected into MA5.8-expressing FLAG-PILRß using the pMx-IRES-GFP retrovirus vector. Transfectants were stained with anti-FLAG mAb and expression of FLAG on GFP-expressing cells is shown (solid line). FLAG expression on a mock transfectant was overlaid in this figure (dotted line). Mean fluorescence intensities of anti-FLAG mAb staining for FLAG-PILRßexpressing cells transfected with DAP12, CD3 , and FcR were 1137.7, 56.4, and 33.9, respectively. (C) IL-2 production by MA5.8 cells and MA5.8 transfected with FLAG-PILRß and DAP12, CD3 , or FcR and cultured for 1 d in the presence of immobilized anti-FLAG mAb (shaded bar) or control mAb (unshaded bar). IL-2 in the culture supernatants was measured by ELISA.
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Next, we stimulated FLAG-PILRßtransfected MA5.8 cells and FLAG-PILRß and DAP12 cotransfected MA5.8 cells with immobilized anti-FLAG mAb (Fig. 2 C). MA5.8 cells expressing FLAG-PILRß plus DAP12 were activated to produce IL-2 upon crosslinking of FLAG-PILRß; however, IL-2 was not induced in cells transfected with FLAG-PILRß alone, FLAG-PILRß plus CD3
, or FLAG-PILRß plus FcR
. Together with the results that PILRß was originally cloned as a molecule that induces cell surface expression of FLAG-DAP12, DAP12 associates with PILRß and is required for high levels of cell surface expression and signal transduction of PILRß.
Tissue Distribution of PILR
and PILRß Transcripts.
We analyzed the expression of PILR
and PILRß transcripts by real-time quantitative PCR. Expressions of PILR
and PILRß were relatively high in lung, liver, and spleen (Fig. 3 A). There was no significant difference in the tissue distribution between PILR
and PILRß when analyzing their expression in total organs. We analyzed the expression of PILRß in various purified populations (Fig. 3 B). Transcripts of PILRß were detected not only in NK cells but also in BM-DCs, peritoneal macrophages, and granulocytes. Because DAP12 that is required for signaling of PILRß is widely distributed not only in NK cells but also in cells of the myeloid lineage, including macrophages, granulocytes, and DCs, PILRß may play a role in the activation of these populations. On the other hand, macrophages and granulocytes also expressed the inhibitory PILR
. In contrast, NK cells and BM-DCs expressed a very low amount of PILR
transcripts. Therefore, the expression pattern of PILR
and PILRß seems to vary depending on cell types and their activation status.
Molecular Cloning of the Ligand for PILRß.
To identify the ligand for these receptors, we generated Ig fusion proteins containing the extracellular domains of PILR
and PILRß. When various transformed cell lines were stained with the PILR
-Ig and PILRß-Ig fusion proteins, we found that some T cell lines, such as EL-4 and 2B4, were stained with both PILR
-Ig and PILRß-Ig (Fig. 4). These Ig fusion proteins did not stain other cell lines, such as B cell and macrophage lines. Because both EL-4 and 2B4 are IL-4producing T cell lines, this suggested that expression of the ligand for PILRß might correlate with Th2 differentiation.

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Figure 4. Expression of PILR-L. The cell lines indicated were stained with PILRß-Ig (solid line), PILR -Ig (dashed line), or control Ig (dotted line), followed by PE-conjugated goat antihuman IgG. Fluorescence intensities of PE-stained cells are shown.
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To elucidate the function of PILRß, we sought to identify the ligand by expression cloning. Because EL-4 was stained well by PILRß-Ig (Fig. 4 A), we constructed a retrovirus cDNA library from EL-4 and undertook expression cloning using PILRß-Ig. The EL-4 retrovirus cDNA library was transfected into Ba/F3 cells, which did not stain with the PILR
-Ig or PILRß-Ig, and Ba/F3 cells expressing the ligand for PILRß were purified by using a fluorescence-activated cell sorter. Single cell clones that were stained with PILRß-Ig were obtained and genes derived from the cDNA library were amplified by PCR using primers corresponding to the flanking retrovirus long terminal repeat and sequenced. Fig. 5 A shows the nucleic acid and predicted amino acid sequence of a PILR-L. PILR-L is a type I membrane protein with a signal sequence, a small extracellular domain, a transmembrane region, and a short 17amino acid cytoplasmic domain with no evident signaling motifs. The sequence of PILR-L was remarkable in two aspects. Surprisingly, the GC content of PILR-L is 75%; GC-rich transcripts are rare in mammalian cells. In addition, the short extracellular domain of PILR-L contained no sites for N- or O-linked glycosylation, again unusual for proteins expressed on the cell surface. PILR-L showed 45% similarity to human CD99, 36% to human CD99L2, and 32% to mouse CD99L2 (26). This indicates that PILR-L belongs to CD99 family.
When the PILR-L was transfected into Ba/F3 cells or 293T cells, the transfectants were stained well with PILRß-Ig (Fig. 5 B). Furthermore, these PILR-L transfectants were also stained with PILR
-Ig. This indicated that PILR-L is a ligand for both PILR
and PILRß. Indeed, when PILR-L was precipitated from cell lysates of PILR-Ltransfected Ba/F3 cells using PILR
-Ig and PILRß-Ig, PILR-L was clearly detected as a 17-kD molecule, although PILRß-Ig precipitated much less PILR-L. A control Ig did not precipitate the 17-kD protein, and this protein was not immunoprecipitated from untransfected Ba/F3 cells (Fig. 5 C). The actual molecular weight was the same as the predicted molecular weight of PILR-L based on the deduced amino acid sequence. We analyzed the expression of PILR-L transcripts by real-time quantitative PCR. PILR-L transcripts were present in most tissues, and expressions of PILR-L were relatively high in lung, spleen, thymus, liver, and spinal cord (Fig. 6).

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Figure 6. Transcription of PILR-L in various tissues. Transcription of PILR and PILRß in various tissues was analyzed by real-time quantitative RT-PCR. Transcription of ß-actin was used as a standard and relative expression levels of PILR-L to the amounts present in spleen are shown. S. intestine, small intestine.
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Cytotoxicity by NK Cells against PILR-Lexpressing Cells.
To examine the function of mouse PILRß and PILR-L, we transfected mouse PILRß into NKL, a human NK cell line, and analyzed cytotoxicity of PILRß-transfected NKL cells against mouse PILR-Ltransfected 293T cells. As shown in Fig. 7 A, mouse PILRß-transfected NKL cells showed cytotoxicity against PILR-Ltransfected 293T, but not against parental 293T cells. Mock-transfected NKL did not show cytotoxicity against PILR-Ltransfected or parental 293T cells, indicating that NKL cells do not express endogenous activating receptors that can recognize the mouse PILR-L. In addition, soluble recombinant human PILR-Ig did not bind to cells expressing mouse PILR-L (unpublished data). These data indicate that mouse PILRß recognizes PILR-Lexpressing cells and transduces an activating signal into NK cells. We observed that purified mouse PILR
-Ig and PILRß-Ig did not block cytotoxicity mediated by mouse PILRß-expressing NKL cells against PILR-L transfectants, probably due to insufficient affinity of these fusion proteins (unpublished data).

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Figure 7. Cytotoxicity of NK cells against PILR-Lexpressing cells. (A) Cytotoxicity of mouse PILRß-transfected human NKL (left) or mock-transfected NKL cells (right) against mouse PILR-Ltransfected 293T cells (closed circles) or parental 293T cells (open circles) is shown. (B) Cytotoxicity of IL-2 expanded NK cells from wild-type mice (left) or DAP12-deficient mice (right) against PILR-Ltransfected Ba/F3 cells (closed circles) or parental Ba/F3 cells (open circles) is shown.
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Next, we analyzed cytotoxicity of IL-2expanded mouse NK cells against mouse PILR-L transfectants. Because mouse Ba/F3 proB cells are resistant to cytotoxicity mediated by mouse NK cells and do not express PILR-L, we transfected PILR-L into Ba/F3 and used these cells as targets. When cytotoxicity of C57BL/6 NK cells against the mouse PILR-L transfectants was analyzed, NK cells showed significant cytotoxicity against PILR-L transfectants compared with the parental Ba/F3 cells (Fig. 7 B). We analyzed NK cells from DAP12-deficient mice (17) because DAP12 is required for signal transduction by PILRß (Fig. 2). As predicted, NK cells from DAP12-deficient mice showed almost the same cytotoxicity against PILR-Ltransfected Ba/F3 cells and parental Ba/F3 cells. These data indicate that the DAP12-associated PILRß is involved in target cell recognition by NK cells.
Activation of DCs by PILR-Lexpressing Cells.
As shown in Fig. 3, BM-DCs, as well as NK cells, express the activating PILRß but express relatively very low amounts of inhibitory PILR
transcripts compared with macrophages and granulocytes. This suggested that PILR-Lexpressing cells might activate not only NK cells but also DCs. Therefore, we analyzed the function of PILRß on BM-DCs. Because DCs are known to produce nitric oxide and TNF
upon activation (27), we analyzed production of nitric oxide and TNF
production by DCs after coculture with PILR-Lexpressing cells. When BM-DCs were stimulated with PILR-L transfectants, DCs produced significant amounts of nitric oxide and TNF
(Fig. 8). In contrast, DCs did not produce nitric oxide and TNF-
when they were cocultured with parental 293T cells lacking PILR-L. These data indicate that PILRß is also involved in the activation of DCs.
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Discussion
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In the present work, we have described a mouse orthologue of human activating PILRß. Furthermore, we identified its ligand by using PILRß-Ig fusion protein. Expression of PILRß was significantly up-regulated in the presence of DAP12, and PILRß-expressing cells were activated only in the presence of DAP12. NK cells showed significant cytotoxicity against PILR-Lexpressing cells, whereas NK cells from mice genetically lacking DAP12 did not. These results indicate that DAP12 mediates signal transduction for PILRß.
Transcription of PILRß was observed not only in NK cells but also in macrophages, DCs, and granulocytes. Because DAP12 is widely expressed in these populations, PILRß may play an important role in the activation of various cell populations. DCs showed significant production of nitric oxide and TNF-
when they were cocultured with PILR-Lexpressing cells, indicating that PILRß directly recognizes PILR-Lexpressing cells and delivers activation signals in various cell types. Of note, DAP12-deficient mice have been previously shown to have a defect in APC function (17, 28), manifested by the accumulation of DCs in the skin, an impaired delayed-type hypersensitivity response and resistance to the induction of myelin oligodendrocyte glycoprotein peptideinduced experimental autoimmune encephalomyelitis. Considering the finding that PILR-L is expressed on T cell lines, there is the possibility that interactions between PILRß and PILR-L are involved in some of the disorders observed in the DAP12-deficient mice.
It is interesting that both the inhibitory PILR
and the activating PILRß recognize the same ligand. Earlier analyses have indicated that human PILR
can inhibit ITAM-mediated activation signals (15, 16). However, activation of NK cells and DCs by coculture with PILR-Lbearing cells indicates either a subset of NK cells or DCs bear only the activating receptor or that the activating receptor is dominant in cells that coexpress both PILR
and PILRß. Indeed, transcription levels of inhibitory PILR
in NK cells and DCs were very low compared with macrophages and granulocytes (Fig. 3 B). Specific mAbs that distinguish PILR
and PILRß are needed to define the precise distribution of these receptors and to study their function in immune responses.
KIR, Ly49, NKG2/CD94, and NKR-P1 are paired receptors expressed by NK cells (8, 11). Similarly, myeloid and B cells have been shown to express the paired receptors, signal-regulatory protein, CD85 (also called Ig-like transcript and leukocyte Ig-like receptor), and paired Ig-like receptor (8, 11). PILR
and PILRß represent another example of related genes, conserved in mice and humans, that encode activating and inhibitory receptors. Mouse PILR
and PILRß are localized on chromosome 5, and human PILR
and ß are localized on chromosome 7 (based on information derived from the National Center for Biotechnology Information Genome BLAST website). PILR
and PILRß are located very closely on the genome, similar to other paired receptors. Although the amino acid homology between the extracellular domains of the paired activating and inhibitory receptors are often very high, often the activating receptor either fails to bind the ligand of the inhibitory receptor or do so with much reduced affinity. This is the case with KIR and Ly49 receptors for MHC class I (29, 30). Similarly, certain inhibitory CD85 receptors bind MHC class I, whereas the activating CD85 receptors do not (31, 32). Further studies are required to determine the precise affinity of mouse PILR
and PILRß for PILR-L; however, we suspect that PILRß will likely be lower than PILR
.
PILR-L exhibits 40% homology to human CD99. Although human CD99 is present on the pairing region of the human X and Y chromosomes, as yet the genomic localization of PILR-L has not been identified. Although it is premature to conclude that PILR-L is a mouse orthologue of CD99, there is a possibility that PILR-L is a mouse orthologue for human CD99. Human CD99 is widely expressed on most hematopoietic cells (34). Similarly, we detected mouse PILR-L transcripts in most tissues, suggesting a broad distribution (Fig. 6). In addition, human CD99 has been reported to be involved in apoptosis of T cells (33) and migration of monocytes through epithelial cells (34) by as yet undefined mechanisms. Expression of mouse PILR-L on the EL-4 and 2B4 T cell lines suggests a potential role in the regulation of T cell immune responses. In studies currently in progress, we have observed that naive CD4+ T cells do not express PILR-L, but interestingly, when naive CD4+ T cells were stimulated with Ag plus APC, PILR-L was expressed on activated CD4+ T cells (unpublished data). This finding, together with expression of PILRß on DCs (Fig. 3 B), suggests the possibility of a new pathway to regulate T cellAPC interactions and influence helper T cell differentiation.
What is the rationale for the evolution of paired immune receptors with activating and inhibitory function? We have proposed the hypothesis that pathogens may exploit inhibitory receptors by acquiring ligands that can dampen immune responses, but that the host may convert the inhibitory immune receptors to activating immune receptors by gene conversion due to selective pressure imposed by the pathogen. An example is provided by the paired inhibitory and activating Ly49 receptors on mouse NK cells that recognize a viral glycoprotein encoded by MCMV (9). According to this hypothesis, there is a possibility that the paired PILRs may recognize certain molecules derived from a pathogen. It is interesting to note that amino acid sequence of PILR-L demonstrates 20% homology with the PEPro-Glu polymorphic GC-rich repetitive sequence of Mycobacterium tuberculosis (35). Although further studies are necessary to reveal the biological role of PILR and PILR-L, our present findings provide evidence for a new receptorligand system involved in both the innate and adaptive immune responses.
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Acknowledgments
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We would like to thank A. Sakamoto, M. Unnno, and R. Shiina for technical assistance and Y. Kurihara and H. Yamaguchi for secretarial assistance.
This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture (to T. Saito and H. Arase) and by the Uehara Memorial Foundation (to H. Arase). K. Ogasawara is supported by a Human Frontier Science Program Long-term fellowship. L.L. Lanier is an American Cancer Society Research Professor and is supported by National Institutes of Health grant CA89294 and the Sandler Family Supporting Fund.
Submitted: 3 November 2003
Accepted: 16 December 2003
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