By
§
From the * Departments of Ophthalmology and Visual Sciences and the Department of Molecular
Microbiology, the § Center for Immunology, and the
Department of Pathology, Washington University
School of Medicine, St. Louis, Missouri 63110
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Abstract |
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Mechanisms responsible for neuroattenuation of herpes simplex virus (HSV) have been defined
previously by studies of mutant viruses in cultured cells. The hypothesis that null mutations in
host genes can override the attenuated phenotype of null mutations in certain viral genes was
tested. Mutants such as those in infected cell protein (ICP) 0, thymidine kinase, ribonucleotide
reductase, virion host shutoff, and ICP34.5 are reduced in their capacity to replicate in nondividing cells in culture and in vivo. The replication of these viruses was examined in eyes and
trigeminal ganglia for 1-7 d after corneal inoculation in mice with null mutations (/
) in interferon receptors (IFNR) for type I IFNs (IFN-
/
R), type II IFN (IFN-
R), and both type
I and type II IFNs (IFN-
/
/
R). Viral titers in eyes and ganglia of IFN-
R
/
mice were not
significantly different from congenic controls. However, in IFN-
/
R
/
or IFN-
/
/
R
/
mice, growth of all mutants, including those with significantly impaired growth in cell culture,
was enhanced by up to 1,000-fold in eyes and trigeminal ganglia. Blepharitis and clinical signs of infection were evident in IFN-
/
R
/
and IFN-
/
/
R
/
but not control mice for all viruses. Also, IFNs were shown to significantly reduce productive infection of, and spread from intact, but not scarified, corneas. Particularly striking was restoration of near-normal trigeminal
ganglion replication and neurovirulence of an ICP34.5 mutant in IFN-
/
R
/
mice. These
data show that IFNs play a major role in limiting mutant and wild-type HSV replication in the
cornea and in the nervous system. In addition, the in vivo target of ICP34.5 may be host IFN
responses. These experiments demonstrate an unsuspected role for host factors in defining the
phenotypes of some HSV mutants in vivo. The phenotypes of mutant viruses therefore cannot
be interpreted based solely upon studies in cell culture but must be considered carefully in the
context of host factors that may define the in vivo phenotype.
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Introduction |
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In humans and experimental animals the pathogenesis of herpes simplex virus (HSV)-11 occurs in a series of discrete stages (1). Acute viral replication at a peripheral site such as the cornea is followed by viral entry into neuronal termini. Corneal infection is followed by intra-axonal transport, which moves the virus to the trigeminal ganglia, where further replication may occur before clearance of infectious virus and the establishment of latency. Failure to clear the virus may result in central nervous system infection, encephalitis, and death. Latency may periodically break down in response to certain stimuli, leading to viral reactivation and shedding. The host factors that regulate the discreet stages of pathogenesis are incompletely defined.
In this paper, the role of host IFNs in determining the phenotypes of herpesvirus mutants in vivo is examined. The ability to create recombinant viruses coupled with the availability of a variety of laboratory animal models of HSV infection have elucidated the roles of viral genes in specific stages in pathogenesis (2). Studies with mutant viruses have shown that many HSV genes are dispensable for viral replication in dividing cells (7). However, such mutants often fail to replicate efficiently in nondividing cultured cells. In animal models, such genes often play key roles, particularly in promoting acute replication either at the primary site of infection or in the nervous system (2, 6, 8). To date, studies of HSV mutants have generally assumed, first, that in vivo phenotypes observed are determined solely by specific virus-cell interactions, and second, that the function of the gene (for example, promotion of efficient replication in nondividing cells) demonstrated in cell culture must correlate with and account for phenotypic differences observed in vivo. Only a few studies have examined a role for the host immune system in influencing the in vivo phenotypes of HSV strains and mutants. For example, infected cell protein (ICP)47 has been shown to influence neurovirulence by blocking CD8+ T cell responses, and certain large genomic fragments of HSV-1 and HSV-2 strains have been shown to confer resistance to IFNs, although specific genes have not been identified (5, 11, 12). This line of investigation is especially relevant to those viruses whose growth in culture is significantly reduced due to mutation of a transactivation function such as ICP0 (13, 14), or a DNA replication function such as ribonucleotide reductase (rr) or thymidine kinase (tk), without which the virus cannot thrive in nondividing cells (15, 16). Restoration of normal virulence to attenuated viral mutants by deletion of specific host factors defines these factors as likely targets of the viral gene in question. With these considerations in mind, the hypothesis was tested that the host IFN response may be an additional, or even strongly determining, influence on the phenotype of herpesvirus mutants.
For these studies, HSV mutants were selected to represent genes from each kinetic class and a variety of functions (Table I). All mutants examined have been intensively characterized in vitro and in vivo and their phenotypes proven to be due to mutation of the targeted gene either through marker-rescue or examination of independently isolated mutants. These mutants are capable of replication in cell under some conditions. However, in nondividing cells some of these mutants are significantly impaired for replication (Table I). All of them have been shown to play important roles in pathogenesis and replication in vivo.
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Infected cell protein (ICP) 0, an immediate-early gene, nonspecifically stimulates the expression of all classes of HSV and cellular genes at the transcriptional level (17). ICP0 is packaged in the virion and after infection colocalizes with and redistributes nuclear domain 10 antigens, and also attenuates DNA-dependent protein kinase activity (22, 23). ICP0 null mutant viruses exhibit significantly reduced replication in culture, especially at low multiplicities of infection and at certain points in the cell cycle (24). The critical role of ICP0 in regulating viral and host gene expression is thought to be sufficient to explain the fact that ICP0 mutant viruses are severely compromised in animal models, exhibiting impaired replication, establishment of latency, and reactivation (2, 25). A role for host factors in determining the in vivo phenotype of ICP0 mutants has not been demonstrated.
The early genes tk and rr are two of several nucleotide metabolism enzymes that allow HSV to replicate autonomously of the cell-cycle status of the host cell. Viral tk phosphorylates deoxypyrimidine nucleosides and rr catalyzes the reduction of ribonucleoside triphosphates (26, 27). Both genes are dispensable in dividing cells, but are essential for viral replication in nonmitotic cells, where it is thought that cellular homologues are not present in sufficient quantity to compensate for rr or tk null mutations (10, 15). In mice, mutants in rr or tk are significantly impaired for replication in the cornea, although the extent of impairment for rr mutants depends on the nature of the mutation (4, 6, 28). However, in all cases mutant viruses are cleared more rapidly than are wild-type virus. Reduced replication in eyes has been proposed to be due to an inability of rr mutants to replicate efficiently at 38°C in mouse cells, coupled with the nonmitotic status of much of the corneal epithelium (28). In the adult nervous system, replication of rr and tk mutants is undetectable and these mutants can establish latency but are unable to reactivate. The lack of replication in the nervous system is clearly consistent with the limited nucleotide metabolism in neurons, and a role for host factors in determining the in vivo phenotypes of rr or tk mutants has not been demonstrated.
Mutants in virion host shutoff (vhs) and ICP34.5 were of particular interest in these studies, primarily because the highly significant degree of attenuation of such mutants in vivo suggests a strong role for host factors in determining the mutant phenotype given their near wild-type replication in culture (9, 29). vhs is a tegument protein that induces the destabilization of host mRNA, leading to rapid cessation of host protein synthesis after infection (30). vhs also degrades viral mRNAs and serves to facilitate the transition of viral gene expression from one kinetic class to the next. The effect of vhs deletion on viral replication in cell culture is modest, but is very significant in mice, in the cornea, trigeminal ganglion, and the brain (29). The poor replication at the periphery probably explains the requirement for vhs for the efficient establishment of latency (31). ICP34.5 shows homology to the DNA damage protein GADD34 and acts by precluding the shutoff of protein synthesis associated with apoptosis by regulation of the IFN-inducible double-stranded RNA-dependent protein kinase R (PKR) pathway (32, 33). The effect of deletion of ICP34.5 upon replication in permissive cells is minimal, but ICP34.5 mutants are impaired for replication in the cornea (9, 34, 35). More importantly, these mutants are profoundly neuroattenuated in trigeminal ganglia after corneal infection, and in the brain after intracranial injection (9, 35, 36). It is possible that the replication of ICP34.5 mutants may be cell cycle dependent, explaining why such mutants fail to replicate efficiently in postmitotic or quiescent cells. Specific host factors that influence the phenotypes of vhs or ICP34.5 mutants in vivo have not been identified fully.
The immune response to acute HSV infection involves
both innate and acquired immunity. The fact that all mutants examined in this study show striking attenuation
within 1 to 2 d after infection suggested a pivotal role for
mediators of innate immunity. Key mediators of innate resistance to viral infection include the IFNs, although the
precise mechanisms by which they exert their effect upon
HSV infection is not fully understood. IFN- has been
shown to inhibit the onset of immediate-early HSV gene expression, and in mice IFN-
is a potent inhibitor of replication in the cornea (37). In addition, IFN-
/
serves
to activate host defenses such as NK cells, which have
themselves been shown to be important in controlling
HSV infection and pathology (41). IFN-
/
has also been
suggested to be important for limiting progress of infection
from peripheral tissues to the nervous system (42). IFN-
has been perhaps the most extensively studied of the IFNs in HSV infection. IFN-
has been shown to vary widely in
its efficacy in reducing HSV growth depending on its target
cell, and in culture, HSV replication appears less sensitive
to inhibition by IFN-
than to IFN-
/
(43). However, it
appears that IFN-
may play an important role in the clearance of HSV from the cornea and in resistance to encephalitis, possibly by inhibiting apoptosis of neurons (44, 45).
In this study, mice that are genetically altered in their abilities to respond to IFNs by virtue of null mutations in IFNRs were corneally infected with mutant viruses whose ability to replicate in normal mice has been shown to be significantly reduced either in corneas or trigeminal ganglia, or both. The data indicate that the phenotypes of a variety of viruses is significantly impacted by the ability of the infected mice to respond to IFNs. The phenotype of HSV mutants therefore must always be interpreted with careful regard to the host's immune response.
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Materials and Methods |
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Viruses and Cells.
African green monkey kidney (Vero) cells were propagated and growth and assay of all virus strains was done as previously described (46). The KOS strain of HSV-1 was the background for mutant viruses dlx3.1 (ICP0 deletion termed hereMouse Strains.
All animal handling and procedures were in compliance with Federal and University policies. Mice were housed and bred in the Washington University School of Medicine biosafety level 2 animal facility, where sentinel mice were screened every 3-6 mo for mouse pathogens and determined to be negative. All mice used in this study were in a pure 129 Ev/Sv (referred to as 129) background and 129 mice were used as immunocompetent controls. Mice deleted for IFNRs used were IFN-Animal Procedures.
6-8-wk-old mice were anesthetized with xylazine and ketamine, corneas were scarified with 10 interlocking strokes with a 27-gauge needle, and 5 µl of medium containing 2 × 104 or 2 × 106 PFU of virus was added per eye. Lids were massaged together briefly to promote adsorption of virus. Eye swab and trigeminal ganglion assays of acute infection were performed as previously described (29). In brief, for eye swabs, eyes were proptosed and moistened cotton swabs used to sample the surface of the eye, going three times in a circular fashion around the eye and twice across the eye making an "x", before placing the swab in 1 ml of tissue culture medium. Sampled trigeminal ganglia were homogenized in 1 ml of tissue culture medium using 1-mm beads in a Mini-Beadbeater-8 (Biospec Products). All samples were titered using standard plaque assay on Vero cells. All data presented are logarithmic means ± SEM and the limit of detection was 10 PFU. Clinical signs of infection (eyelid swelling and loss of facial hair) were measured on a semiquantitative scale of 0-4 as previously described (49). All mouse procedures conformed to protocols approved by the Washington University Animal Studies Committee. ![]() |
Results |
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The replication of
KOS and other mutants used in this study has not been
studied previously in strain 129 mice. Therefore, it was important to determine that the patterns of growth seen in
other wild-type mouse strains were reproduced in strain
129. The replication in eyes of vhs,
RR, and
0 mutant
viruses was reduced relative to KOS in 129 wild-type animals (Fig. 1 A). The replication of the
RR mutant was
reduced by ~10-1,000-fold and was cleared more rapidly
than KOS. The growth of the
0 and
vhs mutants was
more significantly impaired, up to 10,000-fold relative to
KOS. This is especially notable for the
vhs mutant, whose replication in tissue culture is essentially identical to that of KOS. In trigeminal ganglia of 129 mice, the replication
of all viral mutants was undetectable on day 3 after infection, at which time the titer of KOS was >104 PFU per ml
(Fig. 1 B), day 3 being the peak of viral replication in the
trigeminal ganglion. Taken together, these data show that these mutants exhibit impaired replication in 129 mice relative to wild-type virus in both corneas and trigeminal ganglia, consistent with previously published data (Table I and
references 2, 6, 29).
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Previous work from other
laboratories showed that the deletion of IFN-R had little
impact upon early acute wild-type HSV infection in mice
(50), while others have argued for an important role (44,
45). To examine this further, IFN-
R
/
mice were infected with 2 × 106 PFU with either KOS or
vhs. Consistent with these previous studies, the early acute replication patterns of KOS and
vhs were unaltered whether
examined in 129 or IFN-
R
/
mice (Fig. 1, C and D).
To determine the role of IFN-/
in the reduced growth
phenotypes of the mutant viruses, IFN-
/
R
/
and
IFN-
/
/
R
/
mice were infected in parallel with 129 mice, with wild-type or mutant viruses (Fig. 2, A and B).
In the eyes of the IFNR
/
mice there was an increase in
replication of ~10-fold on days 1-4 after infection for
KOS, relative to growth in 129 mice. For
0 and
RR in
IFN-
/
R
/
and IFN-
/
/
R
/
mice, there was a very
significant enhancement of viral replication, with 104-105
PFU per ml in eye swab samples. At comparable times in
129 mice, titers for these mutants were 100 PFU per ml or
undetectable. These enhanced titers were such that they
were within 10-fold of the titers seen for KOS. Titers for
vhs were enhanced by 10-100-fold in IFN-
/
R
/
and
IFN-
/
/
R
/
mice. Although this enhancement was
significant (P < 0.001 by Student's t test), it was reproducibly less than was seen for
0 and
RR. Overall, the patterns of viral replication were very similar in IFN-
/
R
/
and IFN-
/
/
R
/
mice. Given that the IFN-
/
/
R
/
mice are derived from crossing IFN-
/
R
/
with IFN-
R
/
mice, these data are consistent with observations of
previous work (50) as well as this study, indicating that
IFN-
plays only a minor additional role to IFN-
/
in
controlling early acute infection.
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Initial experiments in IFN-/
R
/
and IFN-
/
/
R
/
mice showed that the replication of
0 and
vhs was undetectable in trigeminal ganglia on day 3 after infection,
normally the peak of viral replication in the ganglion (2).
However, longer time course experiments in IFN-
/
R
/
(data not shown) and IFN-
/
/
R
/
(Fig. 2, C and D)
mice revealed that
0 and
vhs were capable of replication
to readily detectable levels in trigeminal ganglia of IFN-
/
/
R
/
mice when sampled from day 3 onwards, ranging
from 101 to 104 PFU per ml of ganglion homogenate. At
these times, both mutant viruses were undetectable in the
trigeminal ganglia of 129 mice.
Taken together, these data show that all viruses tested,
even those with severe growth restrictions in vivo due to
disparate mutations, can replicate with significantly increased efficiency in IFN-/
R
/
and IFN-
/
/
R
/
mice in both eyes and trigeminal ganglia. Furthermore,
patterns of replication seen in IFN-
/
R
/
mice were
comparable to those seen in IFN-
/
/
R
/
mice.
Viruses containing mutations in tk and ICP34.5 have been extensively characterized in vivo in terms of replication, neurovirulence, and establishment and reactivation of latency. As described in the introduction, explanations for their neuroattenuation have been formulated from a large body of data. Thus, these mutants were examined more closely in this study to assess the impact of IFNs on their phenotypes.
The replication of tk was significantly enhanced (10-
100-fold) in the corneas of IFN-
/
/
R
/
compared with
129 mice (Fig. 3 A). In 129 mice,
tk was cleared by day 5, at which time there was >103 PFU per ml persisting in the
IFN-
/
/
R
/
mice. In addition, blepharitis and periocular lesions became evident in the IFN-
/
/
R
/
mice by
day 7, whereas the 129 mice looked essentially uninfected. Moreover,
tk replication was readily detected in trigeminal ganglia of IFN-
/
/
R
/
mice on days 3, 5, and 9, whereas, consistent with previous observations in wild-type
mice, no virus was detectable in the 129 mice (Fig. 3 B).
|
The ICP34.5 mutant, 34.5, was the only mutant tested
in this study whose corneal replication was only modestly
higher in IFN-
/
R
/
compared with 129 mice, with a
<10-fold increase over the first 3 d of infection (Fig. 4, A
and B). However, the mutant was cleared more rapidly
from 129 than IFN-
/
R
/
mice. Similar to wild-type
KOS, the marker-rescued virus
34.5R showed >10-fold
increased replication in IFN-
/
R
/
eyes compared with
129 mice. Replication of
34.5 was not detectable in ganglia
of 129 mice at any time (Fig. 4 C). In contrast, replication of
34.5 in the trigeminal ganglia of IFN-
/
R
/
mice was
robust with a titer of 104 PFU per ml on day 3 and 3 × 104
on day 5, these titers being at most 10-fold less than its
marker-rescued virus
34.5R (Fig. 4 D). Moreover, all
IFN-
/
R
/
mice infected with
34.5 that were not killed
for trigeminal ganglion data died on or before day 9 after infection with symptoms consistent with viral encephalitis.
Data comparable to those seen for
34.5 and
34.5R replication in IFN-
/
R
/
mice were also observed in corneas
and ganglia of IFN-
/
/
R
/
mice (data not shown).
|
Corneal scarification is a prerequisite for efficient uptake of
HSV and other materials in normal mice such as horseradish peroxidase from the cornea into the trigeminal ganglion
(51). In the absence of scarification, replication of KOS
both at the cornea and in ganglia is extremely limited (Strelow, L.I., and D.A. Leib, unpublished data, and Fig. 5).
Therefore, infection of neurons is thought to be the result
of direct uptake into axonal termini coupled with local amplification and subsequent retrograde axonal transport of
virus. Since IFNs played a key role in determining the corneal phenotype of even very attenuated viruses, it was of
interest to assess whether corneal scarification is actually needed for establishment of HSV corneal infection in the
absence of IFN responsiveness. Control 129 and IFN-/
/
R
/
mice were either scarified or not and infected with
2 × 106 PFU of KOS. In 129 mice, there was robust replication of KOS in scarified corneas, with titers >2 × 104
PFU per ml 4 d after infection (Fig. 5 A). In contrast, replication in the nonscarified group was undetectable with one
virus-positive sample (out of eight) on day 2 after infection.
However, in IFN-
/
/
R
/
mice, replication was readily
detected in both scarified and nonscarified mice, although
there was a delay in onset of replication in the nonscarified
group. The level of replication in the scarified IFN-
/
/
R
/
mice was significantly higher than in the nonscarified mice at all time points tested. A similar pattern of replication emerged in the trigeminal ganglia (Fig. 5 B). In 129 mice, replication was virtually undetectable in nonscarified
mice, but robust in scarified mice. In IFN-
/
/
R
/
mice,
replication was readily detectable for both scarified and nonscarified groups, but there was a delay in onset of replication in the nonscarified group. By day 4, titers of virus in
the nonscarified IFN-
/
/
R
/
mice were comparable to
those of scarified 129 mice. These data show that the IFNs
play a key role in determining the outcome of viral infection of the intact cornea.
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Discussion |
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The results presented in this study indicate that the acute
replication patterns in eyes and trigeminal ganglia of both
mutant and wild-type viruses tested are significantly impacted by IFNs. In particular, this study underscores the
critical role played by IFN-/
in the control of early acute
replication of HSV, and the relatively minor role of IFN-
.
These data are consistent with those of Su et al. (52), who
showed that neutralizing antibody-mediated depletion of
IFN-
/
led to a >1,000-fold increase in ocular viral titers
and a corresponding increase in corneal opacity after infection with wild-type HSV-1. Correspondingly, the data of
Cantin et al. (50) showed a less than twofold increase in viral titers in trigeminal ganglia of IFN-
R
/
mice compared with control isogenic mice. These protective effects of IFN-
/
in mice are likely to be due to induction of an
antiviral state in target cells coupled with activation of
other nonspecific host defenses such as NK cells and probably represents the first line of defense against HSV infection. With respect to NK cells, one study showed that resistance to acute wild-type HSV-1 infection correlated to
early IFN production but not NK cell activity, suggesting that NK cell activation is not the primary mechanism of
IFN-mediated resistance (53). A specific role for IFNs is
further supported by the observation that, in contrast to data
presented here for IFN-
/
R
/
mice, tk and ICP34.5 mutants are incapable of significant replication in the nervous
system of SCID mice, which lack adaptive immunity but
express and respond to IFNs (54).
The behavior of the tk and ICP34.5 mutants in IFN-/
R
/
and IFN-
/
/
R
/
mice is of particular interest
and worthy of further discussion. Both of these genes have
been extensively studied and shown by several independent
laboratories to be critical determinants of viral replication
in the nervous system and to be essential for efficient peripheral neuroinvasion. tk mutants in the mouse eye model
typically show robust (albeit reduced relative to wild-type virus) replication in the cornea, with little or no replication observed in the trigeminal ganglion (4). However, tk
mutants do establish latency, as judged by the presence of
viral DNA in the ganglion, although with 10-fold reduced
efficiency (57). Reactivation, however, is minimal or absent. The lack of tk mutant virus replication and reactivation in neurons is consistent with the absence of complementary host tk activity in nondividing cells. How, then, is
a tk mutant able to replicate in the nervous system of
IFNR
/
mice as shown in this study? One clue comes
from the observations that tk mutants can be made to reactivate by addition of thymidine to the culture medium of
latently infected ganglia and that IFN-
can significantly
reduce steady state levels of thymidine and thymidine metabolites in HSV-infected cells (58, 59). Therefore, it is
possible that the lack of IFN-
/
receptors in the knockout mice leads to higher intracellular levels of thymidine in the nervous system, thereby allowing a tk-deleted virus to replicate to detectable and significant levels. An alternate hypothesis is that the increased viral replication and persistence in the cornea leads to a higher viral load in the
ganglion with a concomitant rise in viral trans-acting factors such as VP16 and ICP0, thereby promoting a lytic infection. This hypothesis seems unlikely since increasing the
infectious dose of mutant virus does not promote replication in the ganglion if the virus is inherently crippled for
replication at that site (Strelow, L.I., and D.A. Leib, unpublished data). In addition, such increased levels of trans-acting factors would probably not be able to overcome a
block in thymidine metabolism and DNA replication.
ICP34.5 mutants exhibit significantly reduced replication in eyes of wild-type mice and minimal replication in
the nervous system after peripheral infection. This lack of
replication and neurovirulence is believed to be due to the
ability of ICP34.5 to preclude the shutoff of protein synthesis by IFN-inducible PKR (32, 33). IFNs induce the
synthesis of PKR in an inactive form, which subsequently
becomes activated by binding of viral doubled-stranded
RNA and homodimerization. Once activated, PKR causes
the phosphorylation of the basal translation initiation factor
eIF2 such that translation and protein synthesis are arrested. ICP34.5 interferes with this host protective pathway
by binding to and redirecting the activity of protein phosphatase 1 to dephosphorylate the basal translation factor
eIF-2
, thereby prolonging protein synthesis and viral replication. Cells infected with ICP34.5
viruses therefore exhibit premature shutoff of protein synthesis. Thus, one hypothesis to explain the data in this study is that the
significantly increased neurovirulence of the ICP34.5 mutant in IFN-
/
R
/
and IFN-
/
/
R
/
mice is due to
reduced intracellular levels of PKR that result from an inability to respond to IFNs. A lack of PKR would result in
low efficiency phosphorylation of eIF-2
and an inability
of the host to shut off protein synthesis, obviating the need
for ICP34.5. This could explain why ICP34.5 mutants exhibit almost wild-type virulence and replication in IFNR-
/
/
mice. This near wild-type replication and lethality
of this severely neuroattenuated virus should be additionally considered in the light of ongoing phase 1 clinical trials
using intracranial injection of ICP34.5 mutants for treatment of human glioma (60). The use of viruses singly deleted in the ICP34.5 locus may present a potentially lethal
challenge to those individuals with any deficiencies in IFN
responses. The use of viruses with double deletions, such as
ICP34.5, in addition to ribonucleotide reductase may
therefore be preferable (61).
The phenotypes of the ICP0 and vhs mutants in IFNR/
mice are also worthy of discussion. With respect to ICP0, it
has been shown that the growth of ICP0 mutants is compensated by a host function which is expressed 8 h after release from G0/G1 cell-cycle arrest (24). In wild-type mice,
the expression and response to IFNs after virus infection
may lead to a nonproliferative state in infected cells. In
IFNR
/
mice, IFNs cannot induce such a nonproliferative state, allowing more cells to remain freely cycling.
Therefore, it is possible that greater replication of ICP0
mutants observed in IFNR
/
mice is due, at least in part,
to an enhanced level of expression of the complementary
host factor due to a higher level of proliferation in infected cells.
The phenotype of the vhs mutant is enigmatic in the
sense that it is the least compromised in terms of its replication in cell culture and yet the most compromised in mice
(29). Moreover, it is the virus whose replication in the cornea is least enhanced by lack of IFN responsiveness. However, the observation of significant replication of vhs in
the trigeminal ganglion is consistent with the idea that the
replication of this mutant is not compromised in the nervous system to the same extent that it is in the cornea. When
vhs can gain access to the nervous system either by
corneal infection of IFN-
/
/
R
/
mice or intracerebral
infection of wild-type mice (29), the virus can replicate to
significant levels. The fact that the mutants tested in
IFNR
/
mice in this study do not behave in the same way
strongly suggests that there is some degree of specificity to
the phenotypes observed and not just a global enhancement
of all viral replication to the same level. There is, therefore,
an IFN-independent impairment of vhs mutant virus replication in vivo. Whether this is related to the observed alterations in MHC class I expression previously reported is
an important issue for further experimentation (62).
The intact cornea is an effective barrier to HSV infection, and thus many experimental eye models employ corneal scarification as a means to promote peripheral infection and nervous system invasion (34, 46, 63). However,
the extent to which virus can penetrate the cornea without
scarification is virus strain dependent. Strain KOS, used in
this study, has been shown in mice and rabbits to be virtually incapable of corneal and nervous system invasion without prior scarification, whereas HSV-1 strain McKrae can
penetrate without scarification (34). The mechanism by
which scarification promotes viral invasion is not known,
but a common assumption is that the mechanical damage
to the corneal epithelium permits direct access to the nerve
termini, allowing a conduit into the peripheral nervous system. The data in this study suggest that major determinants
of corneal resistance to viral invasion are the IFNs, since
KOS was capable of robust replication in corneas and ganglia of nonscarified IFNR/
mice. This is consistent with
the idea that different HSV strains may be differentially susceptible to the effects of IFNs (11, 12).
In conclusion, this study has shown the critical role
played by IFN, especially IFN-/
, in controlling early
acute HSV infection, and has also shown that in the absence of IFN responsiveness mice are highly susceptible to
infection even by very attenuated viruses. These studies
show that the attenuation of viral mutants must be carefully
considered within the context of the immune response, especially early-onset innate immunity. Furthermore, any attenuation of growth of HSV mutants in culture should be
examined with respect to IFNs. The use of these and other
genetically altered mice will now allow study of the interactions of viral genes and cytokines during the establishment, maintenance, and reactivation of viral latency.
![]() |
Footnotes |
---|
Address correspondence to David A. Leib, Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, Box 8096, 660 South Euclid Ave., St. Louis, MO 63110. Phone: 314-362-2689; Fax: 314-362-3638; E-mail: leib{at}am.seer.wustl.edu
Received for publication 25 September 1998 and in revised form 15 December 1998.
1 Abbreviations used in this paper: ICP, infected cell protein; HSV, herpes simplex virus; PKR, double-stranded RNA-dependent protein kinase R; rr, ribonucleotide reductase; tk, thymidine kinase; vhs, virion host shutoff.We acknowledge Priscilla Schaffer (University of Pennsylvania, Philadelphia, PA), Rick Thompson (University of Cincinnati, Cincinnati, OH), and Sandy Weller (University of Connecticut, Farmington, CT) for provision of viral mutants, and Andy O'Guin for technical assistance. We thank Sam Speck, Lynda Morrison, and members of the Leib, Morrison, Speck, and Virgin laboratories for helpful discussions.
This study was supported by grants from the National Institutes of Health (RO1 EY09083 to D.A. Leib and RO1 AI39616 to H.W. Virgin). Core support from the National Institutes of Health (P30-EY02687) and from Research to Prevent Blindness Inc. to the Department of Ophthalmology and Visual Sciences is gratefully acknowledged.
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1. | Wildy, P., H.J. Field, and A.A. Nash. 1982. Classical herpes latency revisited. In Virus Persistence, B.W.J. Mahy, A.C. Minson, and G.K. Darby, editors. Cambridge University Press, Cambridge, UK. 133-167. |
2. | Leib, D.A., D.M. Coen, C.L. Bogard, K.A. Hicks, D.R. Yager, D.M. Knipe, K.L. Tyler, and P.A. Schaffer. 1989. Immediate-early regulatory gene mutants define different stages in the establishment and reactivation of herpes simplex virus latency. J. Virol. 63: 759-768 [Medline]. |
3. | Perng, G.C., E.C. Dunkel, P.A. Geary, S.M. Slanina, H. Ghiasi, R. Kaiwar, A.B. Nesburn, and S.L. Wechsler. 1994. The latency-associated transcript gene of herpes simplex virus type 1 (HSV-1) is required for efficient in vivo spontaneous reactivation of HSV-1 from latency. J. Virol. 68: 8045-8055 [Abstract]. |
4. | Coen, D.M., M. Kosz-Vnenchak, J.G. Jacobson, D.A. Leib, C.L. Bogard, P.A. Schaffer, K.L. Tyler, and D.M. Knipe. 1989. Thymidine kinase negative herpesvirus mutants establish latency in mouse trigeminal ganglia, but do not reactivate. Proc. Natl. Acad. Sci. USA. 86: 4736-4740 [Abstract]. |
5. |
Goldsmith, K.,
W. Chen,
D.C. Johnson, and
R.L. Hendricks.
1998.
Infected cell protein (ICP)47 enhances herpes
simplex virus neurovirulence by blocking the CD8+ T cell
response.
J. Exp. Med.
187:
341-348
|
6. | Yamada, Y., H. Kimura, T. Morishima, T. Daikoku, K. Maeno, and Y. Nishiyama. 1991. The pathogenicity of ribonucleotide reductase-null mutants of herpes simplex virus type 1 in mice. J. Infect. Dis 164: 1091-1097 [Medline]. |
7. | McGeoch, D.J., and P.A. Schaffer. 1993. Herpes simplex virus. In Genetic Maps: Locus Maps of Complex Genomes. 3rd Edition. S.J. O'Brien, editor. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 1.147-1.156. |
8. | Chou, J., and B. Roizman. 1990. Mapping of herpes simplex virus-1 neurovirulence to g1 34.5, a gene nonessential for growth in culture. Science. 250: 1262-1266 [Medline]. |
9. | Bolovan, C.A., N.M. Sawtell, and R.L. Thompson. 1994. ICP34.5 mutants of herpes simplex virus type 1 strain 17syn+ are attenuated for neurovirulence in mice and for replication in confluent primary mouse embryo cell cultures. J. Virol. 68: 48-55 [Abstract]. |
10. | Tenser, R.B., and W.A. Edris. 1987. Trigeminal ganglion infection by thymidine kinase-negative mutants of herpes simplex virus after in vivo complementation. J. Virol. 61: 2171-2174 [Medline]. |
11. | Su, Y.H., J.E. Oakes, and R.N. Lausch. 1993. Mapping the genetic region coding for herpes simplex virus resistance to mouse interferon alpha/beta. J. Gen. Virol. 74: 2325-2332 [Abstract]. |
12. | Narita, M., Y. Ando, S. Soushi, T. Karata, and Y. Arao. 1998. The BglII-N fragment of herpes simplex virus type 2 contains a region responsible for resistance to antiviral effects of interferon. J. Gen. Virol. 79: 565-572 [Abstract]. |
13. | Sacks, W.R., and P.A. Schaffer. 1987. Deletion mutants in the gene encoding the herpes simplex virus type 1 immediate-early protein ICP0 exhibit impaired growth in cell culture. J. Virol. 61: 829-839 [Medline]. |
14. | Stow, N.D., and E.C. Stow. 1986. Isolation and characterization of a herpes simplex virus type 1 mutant containing a deletion within the gene encoding the immediate early polypeptide Vmw110. J. Gen. Virol 67: 2571-2585 [Abstract]. |
15. | Goldstein, D.J., and S.K. Weller. 1988. Herpes simplex virus type 1-induced ribonucleotide reductase activity is dispensable for virus growth and DNA synthesis: isolation and characterization of an ICP6 lacZ insertion mutant. J. Virol. 62: 196-205 [Medline]. |
16. | Field, H.J., and P. Wildy. 1978. The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice. J. Hyg. 81: 267-277 . |
17. | Everett, R.D.. 1984. Transactivation of transcription by a herpes simplex virus product: requirement for two HSV-1 immediate-early polypeptides for maximal activity. EMBO (Eur. Mol. Biol. Organ.) J. 3: 3135-3141 [Abstract]. |
18. | Gelman, I.H., and S. Silverstein. 1985. Identification of immediate-early genes from herpes simplex virus that transactivate the virus thymidine kinase gene. Proc. Natl. Acad. Sci. USA. 82: 5265-5269 [Abstract]. |
19. | O'Hare, P., and G.S. Hayward. 1985. Three trans-acting regulatory proteins of herpes simplex virus modulate immediate-early gene expression in a pathway involving positive and negative feedback regulation. J. Virol. 56: 723-733 [Medline]. |
20. | Jordan, R., and P.A. Schaffer. 1997. Activation of gene expression by herpes simplex virus type 1 ICP0 occurs at the level of mRNA synthesis. J. Virol. 71: 6850-6862 [Abstract]. |
21. | Samaniego, L.A., N. Wu, and N.A. DeLuca. 1997. The herpes simplex virus immediate-early protein ICP0 affects transcription from the viral genome and infected-cell survival in the absence of ICP4 and ICP27. J. Virol. 71: 4614-4625 [Abstract]. |
22. | Maul, G.G., H.H. Guldner, and J.G. Spivack. 1993. Modification of discrete nuclear domains induced by herpes simplex virus type 1 immediate early gene 1 product (ICP0). J. Gen. Virol 74: 2679-2690 [Abstract]. |
23. | Lees-Miller, S.P., M.C. Long, M.A. Kilvert, V. Lam, S.A. Rice, and C.A. Spencer. 1996. Attenuation of DNA-dependent protein kinase activity and its catalytic subunit by the herpes simplex virus type 1 transactivator ICP0. J. Virol. 70: 7471-7477 [Abstract]. |
24. | Cai, W., and P.A. Schaffer. 1991. A cellular function can enhance gene expression and plating efficiencies of a mutant defective in the gene for ICP0, a transactivating protein of herpes simplex virus type 1. J. Virol. 65: 4078-4090 [Medline]. |
25. | Clements, J.B., and N.D. Stow. 1989. A herpes simplex virus type 1 mutant containing a deletion within immediate early gene 1 is latency-competent in mice. J. Gen. Virol. 70: 2501-2506 [Abstract]. |
26. | Jamieson, A.T., and J.H. Subak-Sharpe. 1974. Biochemical studies on the herpes simplex virus-specified deoxypyrimidine kinase activity. J. Gen. Virol. 24: 481-492 [Medline]. |
27. | Bachetti, S., M.J. Everlegh, and B. Muirhead. 1986. Identification and separation of the two subunits of the herpes simplex virus ribonucleotide reductase. J. Virol. 57: 1177-1181 [Medline]. |
28. | Jacobson, J.G., D.A. Leib, D.J. Goldstein, C.L. Bogard, P.A. Schaffer, S.K. Weller, and D.M. Coen. 1989. A herpes simplex virus ribonucleotide reductase deletion mutant is defective for productive, acute and reactivatable latent infections of mice and for replication in mouse cells. Virology. 173: 276-283 [Medline]. |
29. | Strelow, L.I., and D.A. Leib. 1995. Role of the virion host shutoff (vhs) of herpes simplex virus type 1 in latency and pathogenesis. J. Virol 69: 6779-6786 [Abstract]. |
30. | Read, G.S., and N. Frenkel. 1983. Herpes simplex virus mutants defective in the virion-associated shutoff of host polypeptide synthesis and exhibiting abnormal synthesis of a (immediate early) viral polypeptides. J. Virol. 46: 498-512 [Medline]. |
31. | Strelow, L.I., T. Smith, and D.A. Leib. 1997. The virion host shutoff function of herpes simplex virus type 1 plays a role in corneal invasion and functions independently of the cell cycle. Virology. 231: 28-34 [Medline]. |
32. | He, B., J. Chou, D.A. Lieberman, B. Hoffman, and B. Roizman. 1996. The carboxy terminus of murine MyD116 gene substitutes for the corresponding domain of the g134.5 gene of herpes simplex virus to preclude the premature shutoff of total protein synthesis in infected human cells. J. Virol. 70: 84-90 [Abstract]. |
33. | Chou, J., J.-J. Chen, M. Gross, and B. Roizman. 1995. Association of a Mr 90,000 phosphoprotein with protein kinase PKR in cells exhibiting enhanced phosphorylation of translation initiation factor eIF-2 and premature shutoff of protein synthesis after infection with g134.5 mutants of herpes simplex virus 1. Proc. Natl. Acad. Sci. USA. 92: 10516-10520 [Abstract]. |
34. | Perng, G.C., R.L. Thompson, N.M. Sawtell, W.E. Taylor, S.M. Slanina, H. Ghiasi, R. Kaiwar, A.B. Nesburn, and S.L. Wechsler. 1995. An avirulent ICP34.5 deletion mutant of herpes simplex virus type 1 is capable of in vivo spontaneous reactivation. J. Virol. 69: 3033-3041 [Abstract]. |
35. | Spivack, J.G., M.U. Fareed, T. Valyi-Nagy, T.C. Nash, J.S. O'Keefe, R.M. Gesser, E.A. McKie, A.R. MacLean, N.W. Fraser, and S.M. Brown. 1995. Replication, establishment of latent infection, expression of the latency-associated transcripts and explant reactivation of herpes simplex virus type 1 gamma 34.5 mutants in a mouse eye model. J. Gen. Virol. 76: 321-332 [Abstract]. |
36. | Thompson, R.L., and J.G. Stevens. 1983. Biological characterization of a herpes simplex virus intertypic recombinant which is completely and specifically non-neurovirulent. Virology. 131: 171-179 [Medline]. |
37. | Oberman, F., and A. Panet. 1988. Inhibition of transcription of herpes simplex virus immediate early genes in interferon-treated human cells. J. Gen. Virol. 69: 1167-1177 [Abstract]. |
38. | Mittnacht, S., P. Straub, H. Kirchner, and H. Jacobsen. 1988. Interferon treatment inhibits onset of herpes simplex virus immediate-early transcription. Virology 164: 201-210 [Medline]. |
39. | Nicholl, M.J., and C.M. Preston. 1992. Inhibition of herpes simplex virus type 1 immediate-early gene expression by alpha interferon is not VP16 specific. J. Virol. 70: 6336-6339 [Abstract]. |
40. | Balish, M.J., M.E. Abrams, A.M. Pumfery, and C.R. Brandt. 1992. Enhanced inhibition of herpes simplex virus type 1 growth in human corneal fibroblasts by combinations of interferon-alpha and -gamma. J. Infect. Dis 166: 1401-1403 [Medline]. |
41. | Bouley, D.M., S. Kanangat, and B.T. Rouse. 1996. The role of the innate immune system in the reconstituted SCID mouse model of herpetic stromal keratitis. Clin. Immunol. Immunopathol. 80: 23-30 [Medline]. |
42. | Halford, W.P., L.A. Veress, B.M. Gebhart, and D.J. Carr. 1997. Innate and acquired immunity to herpes simplex virus type 1. Virology. 236: 328-337 [Medline]. |
43. | Taylor, J.L., S.D. Little, and W.J. O'Brien. 1998. The comparative anti-herpes simplex virus effects of human interferons. J. Interferon Cytokine Res. 18: 159-165 [Medline]. |
44. | Bouley, D.M., S. Kanangat, W. Wire, and B.T. Rouse. 1995. Characterization of herpes simplex virus type-1 infection and herpetic stromal keratitis development in IFN-gamma knockout mice. J. Immunol. 155: 3964-3971 [Abstract]. |
45. | Geiger, K.D., T.C. Nash, S. Sawyer, T. Krahl, G. Patstone, J.C. Reed, S. Krajewski, D. Dalton, M.J. Buchmeier, and N. Sarvetnick. 1997. Interferon-gamma protects against herpes simplex virus type 1-mediated neuronal death. Virology. 238: 189-197 [Medline]. |
46. | Rader, K.A., C.E. Ackland-Berglund, J. Kelvin, Miller, J.S. Pepose, and D.A. Leib. 1993. In vivo characterization of site- directed mutations in the promoter of the herpes simplex virus type 1 latency-associated transcripts. J. Gen. Virol. 74: 1859-1869 [Abstract]. |
47. | Kaplitt, M.G., J.G. Tjuvajev, D.A. Leib, J. Berk, K.D. Pettigrew, J.B. Posner, D.W. Pfaff, S.D. Rabkin, and R.G. Blasberg. 1994. Mutant herpes simplex virus induced regression of tumors growing in immunocompetent rats. J. Neuro-Oncol. 19: 137-147 [Medline]. |
48. | Muller, U., U. Steinhoff, L.F. Reis, S. Hemmi, J. Pavlovic, R.M. Zinkernagel, and M. Aguet. 1994. Functional role of type I and type II interferons in antiviral defense. Science. 264: 1918-1921 [Medline]. |
49. | Davido, D.J., and D.A. Leib. 1996. Role of cis-acting sequences of the ICP0 promoter of herpes simplex virus type 1 in viral pathogenesis, latency and reactivation. J. Gen. Virol. 77: 1853-1863 [Abstract]. |
50. | Cantin, E.M., D.R. Hinton, J. Chen, and H. Openshaw. 1995. Gamma interferon expression during acute and latent nervous system infection by herpes simplex virus type 1. J. Virol. 69: 4898-4905 [Abstract]. |
51. | Arvidson, B.. 1977. Retrograde axonal transport of horseradish peroxidase from cornea to trigeminal ganglion. Acta Neuropathol. 38: 49-51 [Medline]. |
52. | Su, Y.H., J.E. Oakes, and R.N. Lausch. 1990. Ocular avirulence of a herpes simplex virus type 1 strain is associated with heightened sensitivity to alpha/beta interferon. J. Virol. 64: 2187-2192 [Medline]. |
53. |
Bukowski, J.F., and
R.M. Welsh.
1986.
The role of natural
killer cells and interferon in resistance to acute infection of mice
with herpes simplex virus type 1.
J. Immunol.
136:
3481-3485
|
54. | Valyi-Nagy, T., R.M. Gesser, B. Raengsakulrach, S.L. Deshmane, B.P. Randazzo, A.J. Dillner, and N.W. Fraser. 1994. A thymidine kinase-negative HSV-1 strain establishes a persistent infection in SCID mice that features uncontrolled peripheral replication but only marginal nervous system involvement. Virology. 199: 484-490 [Medline]. |
55. | Valyi-Nagy, T., M.U. Fareed, J.S. O'Keefe, R.M. Gesser, A.R. MacLean, S.M. Brown, J.G. Spivack, and N.W. Fraser. 1994. The herpes simplex virus type 1 strain 17+ gamma 34.5 deletion mutant 1716 is avirulent in SCID mice. J. Gen. Virol. 75: 2059-2063 [Abstract]. |
56. | Heise, M.T., and H.W. Virgin. 1995. The T cell independent role of gamma interferon and tumor necrosis factor alpha in macrophage activation during murine cytomegalovirus and herpes simplex virus infections. J. Virol. 69: 904-909 [Abstract]. |
57. | Kramer, M.F., and D.M. Coen. 1995. Quantification of transcripts from the ICP4 and thymidine kinase genes in mouse ganglia latently infected with herpes simplex virus. J. Virol. 69: 1389-1399 [Abstract]. |
58. | Tenser, R.B., A. Gaydos, and K.A. Hay. 1996. Reactivation of thymidine kinase-defective herpes simplex virus is enhanced by nucleoside. J Virol. 70: 1271-1276 [Abstract]. |
59. | O'Brien, W.J., K. Piotrowski, and J.L Taylor. 1995. Effect of interferon-alpha on the steady-state levels of thymine, thymidine, guanine, and guanosine metabolites in herpes simplex virus-type 1-infected cells. Intervirology 38: 249-255 [Medline]. |
60. |
McKie, E.A.,
D.I. Graham, and
S.M. Brown.
1998.
Selective
astrocytic transgene expression in vitro and in vivo from the
GFAP promoter in a HSV RL1 null mutant vector![]() |
61. | Mineta, T., S.D. Rabkin, T. Yazaki, W.D. Hunter, and R.L. Martuza. 1995. Attenuated multi-mutated herpes simplex virus-1 for the treatment of malignant gliomas. Nat. Med. 1: 938-943 [Medline]. |
62. | Tigges, M.A., S. Leng, D.C. Johnson, and R.L. Burke. 1996. Human herpes simplex virus (HSV)-specific CD8+ CTL clones recognize HSV-2-infected fibroblasts after treatment with IFN-gamma or when virion host shutoff functions are disabled. J. Immunol. 156: 3901-3910 [Abstract]. |
63. | Tenser, R.B., and R.H. Hyman. 1987. Latent herpesvirus infections of neurons in guinea pigs and humans. Yale J. Biol. Med. 60: 159-167 [Medline]. |
64. | Smith, K.O.. 1964. Relationships between the envelope and the infectivity of herpes simplex virus. Proc. Soc. Exp. Biol. Med. 115: 814-816 . |
65. | Goldstein, D.J., and S.K. Weller. 1988. Factor(s) present in herpes simplex virus type 1-infected cells can compensate for the loss of the large subunit of the viral ribonucleotide reductase: characterization of an ICP6 deletion mutant. Virology. 166: 41-51 [Medline]. |