Fc{gamma} Receptor–mediated Phagocytosis in Macrophages Lacking the Src Family Tyrosine Kinases Hck, Fgr, and Lyn

Cheryl J. Fitzer-Attasa,b, Malcolm Lowrya,b, Mary T. Crowleya,b, Alexander J. Finna,b, Fanying Mengc, Anthony L. DeFrancoa,b, and Clifford A. Lowellc
a From the Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
b From the George Williams Hooper Foundation, University of California at San Francisco, San Francisco, California 94143
c From the Department of Laboratory Medicine, University of California at San Francisco, San Francisco, California 94143

Correspondence to: Clifford A. Lowell, Department of Laboratory Medicine, University of California at San Francisco, 513 Parnassus Ave., San Francisco, CA 94143. Tel:415-476-2963 Fax:415-502-6497 E-mail:clowell{at}cgl.ucsf.edu.


  Abstract
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Abstract
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Materials and Methods
Results
Discussion
Acknowledgements
References

Macrophage Fc{gamma} receptors (Fc{gamma}Rs) mediate the uptake and destruction of antibody-coated viruses, bacteria, and parasites. We examined Fc{gamma}R signaling and phagocytic function in bone marrow–derived macrophages from mutant mice lacking the major Src family kinases expressed in these cells, Hck, Fgr, and Lyn. Many Fc{gamma}R-induced functional responses and signaling events were diminished or delayed in these macrophages, including immunoglobulin (Ig)G-coated erythrocyte phagocytosis, respiratory burst, actin cup formation, and activation of Syk, phosphatidylinositol 3-kinase, and extracellular signal–regulated kinases 1 and 2. Significant reduction of IgG-dependent phagocytosis was not seen in hck-/-fgr-/- or lyn-/- cells, although the single mutant lyn-/- macrophages did manifest signaling defects. Thus, Src family kinases clearly have roles in two events leading to Fc{gamma}R-mediated phagocytosis, one involving initiation of actin polymerization and the second involving activation of Syk and subsequent internalization. Since Fc{gamma}R-mediated phagocytosis did occur at modest levels in a delayed fashion in triple mutant macrophages, these Src family kinases are not absolutely required for uptake of IgG-opsonized particles.

Key Words: actin polymerization, Fc{gamma} receptors, macrophage, phagocytosis, Src family kinases


  Introduction
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Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
References

Clustering of Fc{gamma} receptors (Fc{gamma}Rs) occurs upon binding of their ligand, the Fc portion of IgG, which is present in immune complexes or on antibody-coated cells. Fc{gamma}R activation in macrophages results in the secretion of products involved in an inflammatory response, induction of antibody-dependent cellular cytotoxicity, and phagocytosis, which play key roles in our immune defense against infectious diseases (1) (2). Through these processes, Fc{gamma}Rs mediate the ingestion of viruses, bacteria, and parasites, as well as the antibody-dependent killing of cells expressing viral or tumor antigens (3) (4). In addition, internalization of antigens by phagocytosis leads to antigen processing and presentation to neighboring T cells.

Signaling through Fc{gamma}Rs has striking parallels to signaling via Fc{epsilon}RI and the T and B cell antigen receptors (5) (6). These receptors have been classified as members of the multichain immune recognition receptor family, and mediate signaling via homologous cytoplasmic sequences called the immunoreceptor tyrosine-based activation motif (ITAM).1 For class I and class III Fc{gamma}Rs, these sequences are present on the accessory {gamma} chain (and the TCR {zeta} chain for Fc{gamma}RIII), whereas for class II Fc{gamma}Rs they are present on the cytoplasmic portion of the ligand binding chain (7). ITAMs contain paired tyrosines and leucines or isoleucines in the consensus sequence YxxL/I(x)7–12YxxL/I. The ITAM is both necessary and sufficient for many Fc{gamma}R-triggered signaling events and is involved in the recruitment, via Src homology 2 (SH2) domain–phosphotyrosine interactions, and activation of specific tyrosine kinases upon receptor clustering (1).

As has been analogously proposed for B cell and T cell receptors, this clustering of Fc{gamma}Rs by either the onset of phagocytosis or treatment with cross-linking antibodies rapidly stimulates the membrane-associated Src family tyrosine kinases, which in macrophages include Hck, Fgr, and Lyn (8) (9) (10). These kinases are believed to be responsible for phosphorylation of ITAM tyrosines of the Fc{gamma}Rs. Next, the tandem SH2 domains of Syk bind to these newly created docking sites, leading to the phosphorylation and activation of Syk. The Src family tyrosine kinases are also capable of binding the Fc{gamma}R after ITAM phosphorylation and may associate with it or with other receptor subunits at low affinity before receptor stimulation as well (11).

Both Src and Syk family kinases likely contribute to the phosphorylation of targets involved in downstream biochemical processes, such as phosphatidylinositol-4,5-bisphosphate (PIP2) breakdown, phosphatidylinositol 3-kinase (PI 3-kinase) activation, and stimulation of the mitogen-activated protein (MAP) kinase pathways. How these signaling events are connected to biological responses of the cell, including phagocytosis, is poorly understood. Syk has been shown to play a critical role in Fc{gamma}R-mediated phagocytosis, both by the requirement for Syk for enhancement of Fc{gamma}R phagocytic function in transfected Cos cells (12) and by analysis of Syk-deficient macrophages, which are defective in internalization of antibody-coated RBCs (13) (14). Interestingly, syk-/- macrophages are able to bind IgG-coated particles normally and initiate the first steps in the signaling pathway leading to actin polymerization for phagocytic cup formation; however, they are completely unable to fuse the extended membranes around the phagocytic vesicle and hence fail to engulf the particle. Treatment of macrophages with inhibitors of PI 3-kinase produces a similar block in phagocytic vesicle closure (15).

We sought to examine the role of Src family tyrosine kinases in Fc{gamma}R-mediated phagocytosis and signaling by using macrophages from genetically targeted mice lacking one or more of the Src family kinases expressed in these cells. Our results indicate that these Src family kinases are important for Fc{gamma}R-induced formation of actin cups, {gamma} chain phosphorylation, and activation of Syk. Interestingly, in the absence of these Src family kinases, most of these processes were delayed but not completely defective, suggesting that Src family kinases are not absolutely required for Fc{gamma}R-mediated phagocytosis.


  Materials and Methods
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Introduction
Materials and Methods
Results
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Reagents.
The following antibodies and antisera were used in these experiments: supernatants from 2.4G2 (rat anti-Fc{gamma}RII and III) and MAR18.5 (mouse anti–rat Ig {kappa} light chain) were collected from the culture medium of hybridoma cells obtained from American Type Culture Collection; F(ab')2 fragment mouse anti–rat IgG and FITC-conjugated goat anti–rabbit IgG (Jackson ImmunoResearch Laboratories); anti-Cbl, anti–extracellular signal–regulated kinase (Erk)1, and anti–c-Jun NH2-terminal kinase (JNK) (Santa Cruz Biotechnology); anti-JNK1 (PharMingen); anti-Syk (16), anti-{gamma} chain antisera (provided by J.P. Kinet, Harvard University, Boston, MA); antiphosphotyrosine hybridoma 4G10 (17), fluorescein anti–rat IgG2b (PharMingen); and anti-SRBCs (Nordic Immunology). Protein A– and protein G–Sepharose were from Amersham Pharmacia Biotech. Purified ReLPS from Salmonella minnesota was obtained from List Biological Laboratories. Na251CrO4 was purchased from Amersham Pharmacia Biotech, and 32PO4 and [{gamma}-32P]ATP were from NEN Life Sciences.

Macrophages.
Generation of the mouse strains with targeted deletions in hck, fyn, or lyn genes has been described (18) (19) (20). All studies were carried out using bone marrow–derived macrophages cultured as described (20) except that RPMI 1640 medium was used in place of {alpha}-MEM. 20% L cell–conditioned medium (LCM) was used as a source of M-CSF. After 1–2 d in culture, nonadherent bone marrow cells, at concentrations of 0.5 x 105/well or 1 x 106/plate, were transferred to new 24-well or 100-mm plates, respectively. Confluent monolayers of adherent macrophages were used for phagocytosis or signaling experiments 5–7 d later. In some cases, nonadherent cells were frozen in liquid nitrogen and then thawed, counted, and plated as above for experiments 5–7 d later.

Phagocytosis Assays.
SRBCs (Accurate Chemical and Scientific Corp.) were washed in PBS and resuspended to a 5% solution (~109/ml) in RPMI 1640. 1 ml of this solution was incubated with 400 µCi Na251CrO4 for 2–3 h at 37°C. SRBCs were then washed twice in PBS and incubated at room temperature with a subagglutinizing concentration (1:800) of anti-SRBC IgG for 30–45 min at room temperature. After two more washes in PBS, SRBCs were resuspended to the original volume (5% solution) but then further diluted in cold RPMI 1640 medium (1:300) for the phagocytosis assay. These 51Cr-labeled, antibody-coated erythrocytes will be referred to as 51Cr-EAs.

Confluent macrophages, in 24-well plates, were chilled on ice before addition of cold 51Cr-EAs (0.5 ml/well). Plates were centrifuged at 150 g for 5 min at 4°C to promote contact of 51Cr-EAs with the adherent macrophages. Phagocytosis was begun by aspiration of cold medium and addition of warm medium to the wells. Plates were immediately put at 37°C for the indicated time periods. Reactions were stopped by returning plates to the ice, followed by quick aspiration, washing, and hypotonic lysis of uningested 51Cr-EAs with 750 µl water for 1 min. After a subsequent wash in PBS, adherent macrophages with ingested 51Cr-EAs were lysed in 500 µl 1% SDS and transferred to tubes for quantitation of radioactivity in a {gamma}-counter (CliniGamma; LKB-Wallac). Percent phagocytosis was calculated based on the total cpm of replicate wells treated as described above but without hypotonic lysis of uningested 51Cr-EAs. Each time point represents the average of four replicate wells. Internalization of EAs was blocked by known inhibitors of phagocytosis such as cytochalasin D (2 µM), which inhibits actin polymerization, and wortmannin (100 mM), which inhibits the enzyme PI 3-kinase.

Phagocytosis of C3-opsonized FITC-labeled zymosan particles (Molecular Probes) was carried out as described (21). In brief, zymosan particles (5 x 107 beads) were incubated in 100 µl of mouse serum for 30 min at 37°C to fix C3bi onto the surface of the yeast particle. Immunofluorescent staining with anti-C3 polyclonal antisera (Sigma Chemical Co.) confirmed uniform opsonization of the zymosan particles with C3 (not shown). Washed C3-opsonized particles (5 x 105) were added to confluent monolayers of macrophages cultured in 96-well black-sided tissue culture plates, and the plates were centrifuged at 150 g for 30 s and incubated on ice for 30 min. Unbound particles were removed by washing with PBS, and phagocytosis was initiated by addition of medium at 37°C. At the indicated times, plates were removed from the 37°C incubator, the designated wells were washed, and phagocytosis was stopped by addition of 2% paraformaldehyde. The total fluorescence was measured in a fluorescent plate reader (CytoFluor II; PerSeptive Biosystems), after which fluorescence from bound but not internalized zymosan particles was quenched by addition of 2 mg/ml trypan blue in 0.15 M NaCl/20 mM sodium citrate buffer, pH 4.4. The percent phagocytosis was calculated by dividing the value of the trypan blue–quenched signal by the total fluorescence for each well. Each time point was performed in six-well replicates. Attachment of C3-opsonized yeast particles to macrophages was blocked by addition of anti–Mac-1 (CR3; ß2 integrin, CD11b/CD18) mAb (not shown).

Respiratory Burst.
Fc{gamma}R-mediated internalization of immune complexes leading to oxidative burst in the phagosomal vacuole was monitored using the Fc OxyBURST® reagent from Molecular Probes. The reagent consists of insoluble BSA–anti-BSA immune complexes in which the BSA is covalently labeled with dichlorodihydrofluorescein (H2DCF). Oxidation of the H2DCF to DCF in the phagosomal vesicle produces green fluorescence that can be monitored by flow cytometry (22). For this assay, macrophages (either resting or primed by 12-h incubation with 10 ng/ml LPS plus 20 U/ml IFN-{gamma}) were removed from culture dishes and placed in suspension in HBSS at 2 x 106/ml. Suspended cells (500 µl aliquots) were maintained at 37°C for 15 min, then collected by flow cytometry to determine background fluorescence at 530 nm at a rate of ~200 cells/s. Cells were then stimulated with the Fc OxyBURST® reagent at a final concentration of 120 µg/ml, and development of fluorescence was monitored (the reaction tube was maintained at 37°C). Changes in mean fluorescence of the population were plotted at 0.67-s intervals. In some experiments, cells were preincubated with cytochalasin D (3 µM) to inhibit phagocytosis.

Endocytosis of Immune Complexes.
Fc{gamma}R-mediated endocytosis of small soluble immune complexes was determined using flow cytometry to measure the uptake of PE-labeled heat-aggregated IgG. Biotinylated rabbit IgG (250 µl of a 10 mg/ml solution in PBS; Pierce Chemical Co.) was heated to 63°C for 30 min to partially denature and aggregate the immunoglobulins. These complexes were labeled by addition of 5 µg of PE-conjugated streptavidin. Cultured macrophages were placed in suspension in HBSS plus 1% BSA medium at either 4°C or 37°C, then incubated with immune complexes at a final concentration of 250 µg/ml for varying periods of time. At the end of the incubation period, cells were washed in HBSS once and surface-bound immune complexes were removed by treatment with PBS, pH 2.5, for 1 min at 4°C. After an additional HBSS wash, cells were fixed in 1% paraformaldehyde in PBS. Cells were analyzed by flow cytometry to measure endocytosed IgG. In some experiments, cells were preincubated with cytochalasin D (3 µM) before incubation with immune complexes.

Photomicroscopy.
Macrophages grown on coverslips were synchronized on ice for EA phagocytosis as described above. At 5 and 15 min after addition of 37°C medium, the cells were washed twice in PBS then fixed in 3% paraformaldehyde for 15 min on ice. After two more PBS washes, cells were stained with rhodamine-conjugated phalloidin (Molecular Probes) for 30 min at room temperature. Subsequently, cells were washed twice in PBS, then incubated with FITC-conjugated goat anti–rabbit IgG (1:50 dilution) in PBS/1% BSA for 45 min at room temperature. Cells were washed twice in PBS, fixed again in 1% paraformaldehyde for 10 min, then viewed by fluorescence microscopy. Digital images were captured in two fluorescent channels on a DeltaVision fluorescence microscope (Applied Precision).

Cellular Activation, Immunoprecipitation, and Immunoblotting.
Before stimulation, macrophages were placed in growth medium lacking LCM for 3–4 h. Plates were cooled on ice and then incubated for 30–45 min with chilled supernatant from the rat anti-Fc{gamma}R 2.4G2 hybridoma. After washing with PBS, a secondary cross-linking anti–rat antibody (MAR18.5 or F[ab']2 fragment mouse anti–rat IgG) was added at 10 µg/ml in PBS and cells were immediately placed at 37°C to begin stimulation. For LPS or CSF-1 stimulation, cells were starved as above and LPS (1 µg/ml) or LCM was then added directly to culture plates at 37°C. At the indicated times, the stimulated cell monolayers were washed with cold PBS containing 1 mM Na3VO4 and then lysed in 0.7 ml lysis buffer (20 mM Tris-HCl, pH 8, 137 mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA, 2 mM Na3VO4, 10 mM sodium pyrophosphate, 80 mM disodium ß-glycerophosphate, 1 µg/ml aprotinin, 10 µg/ml leupeptin, and 100 µg/ml PefablocTM SC [Boehringer Mannheim Biochemicals]). Plates were rocked at 4°C for 15 min to extract cellular proteins, and lysates were then centrifuged at 17,000 g for 15 min to remove insoluble material. Lysates were precleared with 30 µl of protein G–Sepharose, and protein concentration in the precleared supernatant was determined by the Coomassie blue dye binding assay (Bio-Rad).

For immunoblotting, 30–100 µg of total lysate protein was separated on 8 or 10% SDS-polyacrylamide gels under reducing conditions. For Erk kinase mobility shift assays, lysates were separated on SDS-PAGE gels having an acrylamide/bisacrylamide ratio of 120:1. For immunoprecipitation experiments, 1 µg of anti-Cbl or 5–10 µl of anti-Syk or anti-{gamma} chain antiserum was added to equivalent amounts of lysate protein for 1 h at 4°C, after which 30–40 µl of protein A–Sepharose was added for an additional 2–4 h with constant rocking. For immunoprecipitation with 4G10, hybridoma supernatant was first bound to protein G–Sepharose, and this conjugate was then added to cell lysates. Immunoprecipitates were washed three times in wash buffer (10 mM Tris-HCl, pH 7.5, 140 mM NaCl, 0.1% SDS, 1% NP-40, 10 mM EDTA, 2 mM EGTA, 1 µg/ml aprotinin, 10 µg/ml leupeptin, and 100 µg/ml PefablocTM), resuspended in 1x reducing SDS sample buffer, and separated by SDS-PAGE. After transfer of proteins to nitrocellulose membranes and incubation with unlabeled primary and horseradish peroxidase–labeled secondary antibodies, immunoblots were developed with chemiluminescence reagent (NEN Life Science Products). Stripping of membranes was performed at 50°C for 30 min in a buffer containing 62.5 mM Tris-HCl, pH 6.7, 2% SDS, and 100 mM 2-ME.

For detection of {gamma} chain phosphorylation, macrophages were incubated in phosphate-free RPMI 1640 (Irvine Scientific) containing 1% FCS and 32PO4 (3.1 mCi/100-mm plate) for 5 h at 37°C. Cells were then stimulated as described above, except that incubation with MAR18.5 was performed on ice. Lysates were sequentially immunoprecipitated, first with control anti-Igß antisera and then with anti-{gamma} chain antisera. Washed immunoprecipitates were then separated on a 15% reducing SDS-PAGE gel, and 32PO4-labeled proteins were detected by autoradiography.

JNK and PI 3-Kinase Assays.
Anti-JNK immunoprecipitates were assayed for kinase activity using glutathione S-transferase (GST)-cJun as a substrate in kinase assay buffer (25 mM Hepes, pH 7.6, 20 mM MgCl2, 20 mM disodium ß-glycerophosphate, 1 mM Na3VO4, and 2 mM dithiothreitol) as described (23). PI 3-kinase assays were performed using either antiphosphotyrosine (4G10) or anti-Cbl immunoprecipitates as described previously (24). 32P incorporation into phosphatidylinositol 3-phosphate was quantitated by PhosphorImager® analysis (Molecular Dynamics).


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Impaired Fc{gamma}R-mediated Phagocytosis and Respiratory Burst in hck-/-fgr-/-lyn-/- Macrophages.
To examine the role of Src family tyrosine kinases in Fc{gamma}R-mediated phagocytosis, we studied this process in primary cultures of bone marrow–derived macrophages isolated from mice deficient in the Src family members prevalent in these cells, Hck, Fgr, and Lyn. In initial studies using a nonsynchronous assay in which macrophages were incubated with EAs at 37°C for 20–30 min, we observed no great difference in the phagocytic ability of wild-type versus hck-/-fgr-/-lyn-/- cells, as determined by microscopic counting of internalized phase-bright EAs ((13); and data not shown) or uptake of 51Cr-labeled EAs (see below). However, when initiation of phagocytosis was synchronized by first binding the EAs to macrophages on ice and then placing them at 37°C to start phagocytosis, we noted that the wild-type macrophages internalized bound EA particles quite rapidly (within a few minutes) while the process was delayed in hck-/-fgr-/-lyn-/- macrophages. As shown in Fig 1 A, after 5 min at 37°C, wild-type cells had ingested large numbers of phase-bright EAs, but hck-/-fgr-/-- lyn-/- cells had ingested very few.





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Figure 1. Reduced phagocytosis in hck-/-fgr-/-lyn-/- macrophages. (A) EAs were bound to bone marrow–derived macrophages on ice (0 min) before initiation of phagocytosis at 37°C for the indicated time periods. Uningested EAs were removed by hypotonic lysis, and cells were fixed for photomicroscopy. Bar, 10 µm. (B) Phagocytosis assays were performed as in A with 51Cr-EAs. After hypotonic lysis, the amount of 51Cr-EAs ingested was determined by quantitation of radioactivity in total cell lysates. Data shown are representative of 26 (wild-type) or 22 (mutant) independent experiments on cells pooled from 2–5 mice. Error bars represent the SEM of quadruplicate samples. (C) Phagocytosis of C3-opsonized FITC-labeled zymosan particles. C3-zymosan phagocytosis was initiated by binding of particles to macrophages at 4°C, unbound particles were removed by washing, and 37°C medium was added. Phagocytosis was stopped by addition of 2% paraformaldehyde, and the fluorescence from nonengulfed particles was quenched by addition of trypan blue. Data shown are representative of six independent experiments on cells pooled from two to five mice. Error bars represent the SEM of six determinations per time point. (D) Equivalent expression of Fc{gamma}Rs on wild-type and triple mutant cells as demonstrated by flow cytometry after staining with mAb 2.4G2 (rat anti-Fc{gamma}RII and III, solid line) or control rat IgG2b (broken line).

To measure the rate of phagocytosis accurately, we used an assay for phagocytosis based on the ingestion of 51Cr-labeled EAs (see Materials and Methods). Fig 1 B shows a representative time course experiment in which the delayed onset of phagocytosis in hck-/-fgr-/-lyn-/- macrophages is evident. At 2–10 min, the defect in phagocytosis in mutant macrophages was greatest. At these early time points, while wild-type macrophages had already internalized nearly all of the bound EAs, phagocytosis by hck-/-fgr-/-lyn-/- macrophages was minimal. At later time points, however, the hck-/-fgr-/-lyn-/- macrophages internalized 51Cr-EAs moderately well. By 30 min, wild-type macrophages had internalized 88 ± 3% (n = 26 experiments; ±SEM) of the bound EAs, whereas triple mutant cells phagocytosed a lower fraction of the bound EAs, varying from 13 to over 79% in different experiments (mean of 41 ± 6%, n = 22 experiments; ±SEM). Variation in the triple mutant phagocytosis was seen with different lots of anti-SRBCs used to opsonize RBCs and with different serum samples used for macrophage culture. Poorer phagocytosis by the mutant macrophages was not due to defective recognition of EAs, since both mutant and wild-type macrophages bound EAs similarly on ice (Fig 1 A, 0 time) and displayed equivalent cell-surface levels of Fc{gamma}RII and III as determined by flow cytometry (Fig 1 D). In contrast to Fc{gamma}R-mediated phagocytosis, internalization of complement C3b-bound zymosan particles was equivalent in wild-type and hck-/-fgr-/-lyn-/- bone marrow–derived macrophages (Fig 1 C). Likewise, triple mutant macrophages had no impairment in cytokine or nitrite production after stimulation with LPS, bacterial DNA, or inflammatory cytokines ((20); and data not shown).

The induction of respiratory burst after binding of insoluble immune complexes to Fc{gamma}Rs was also severely impaired in the triple mutant cells (Fig 2 A). Although resting or LPS/IFN-{gamma}–primed wild-type cells displayed robust oxidative burst after ingestion of H2DCF-labeled immune complexes, resting hck-/-fgr-/-lyn-/- cells were completely inactive and LPS/IFN-{gamma}–primed cells mounted a considerably blunted response. The Fc{gamma}R-dependent oxidative burst in this assay was blocked by preincubation of cells with cytochalasin D (not shown), indicating that signal transduction leading to formation of F-actin is required for assembly of the NADPH oxidase within the phagocytic vesicle, as reported previously (22) (25) (26).



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Figure 2. Normal immune complex endocytosis but impaired Fc{gamma}R-mediated activation of respiratory burst in hck-/-fgr-/-lyn-/- macrophages. (A) Suspension macrophages (resting or primed by 12-h incubation in LPS/IFN-{gamma}) were incubated with 120 µg/ml of Fc OxyBURST® immune complexes, and the development of fluorescence resulting from oxidative burst was monitored by flow cytometry. (B) Endocytosis of heat-aggregated rabbit IgG was determined by exposing cells to PE-conjugated immune complexes for varying periods of time, then removing the surface-bound complexes by acid treatment. The amount of remaining fluorescence representing internalized immune complexes was determined by flow cytometry. The data shown are for a 20-min incubation of cells with immune complexes at 37°C (solid lines) or 4°C (broken lines). Equivalent uptake of complexes was seen also in wild-type and mutant cells at earlier and later time points (not shown).

In contrast to phagocytosis and induction of respiratory burst, wild-type and triple mutant macrophages displayed equivalent endocytosis of soluble immune complexes (Fig 2 B). Using a flow cytometric assay for internalization of fluorescently labeled heat-aggregated IgG, we observed equal labeling of wild-type and mutant cells at varying times after exposure to the complexes. Unlike phagocytosis or induction of respiratory burst, internalization of immune complexes was not blocked by preincubation of cells with cytochalasin D (not shown). This is consistent with the observation that phagocytosis of immune complexes requires {gamma} chain signaling to F-actin formation while endocytosis does not (27).

Delayed Actin Cup Formation in hck-/-fgr-/-lyn-/- Macrophages.
An early event in the phagocytic process is the polymerization of actin underlying the membrane with engaged phagocytic receptors (2). Therefore, we examined phagocytic cup formation in wild-type and mutant macrophages by staining cells for polymerized actin with rhodamine-phalloidin. Fluorescein-conjugated anti–rabbit IgG was used to visualize the bound EA particles. In agreement with the delayed phagocytosis seen in hck-/- fgr-/-lyn-/- macrophages, there were very few actin cups formed by mutant cells at 5 min after binding of EA particles, whereas a high fraction of EA particles bound by wild-type macrophages at 5 min had already elicited actin cup formation (Fig 3). By 15 min, some actin cup formation was clearly evident in the mutant macrophages. Thus, in the absence of Hck, Fgr, and Lyn, actin polymerization was slowed but not blocked, correlating closely with what was seen by examining internalization of labeled EA particles.



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Figure 3. Reduced actin cup formation in hck-/-fgr-/-lyn-/- macrophages. Phagocytosis of EAs in wild-type (top panels, Wt) and triple mutant (middle and bottom panels) macrophages was initiated as described in the legend to Fig 1. After 5 min (top and middle panels) or 15 min (bottom panels), unbound EAs were removed by washing and macrophages were fixed in 3% paraformaldehyde. Cells were stained with rhodamine-conjugated phalloidin to detect F-actin at sites of phagocytic cup formation (left), and with FITC-conjugated anti–rabbit IgG to detect the bound SRBCs (middle). An overlay of the F-actin and anti–rabbit IgG staining demonstrates colocalization of bound EAs with phagocytic cups (right). Cells were examined in all focal planes for the presence of actin cups. The middle panels show a focal plane that includes the nucleus of the triple mutant cells to localize cellular structures, since these cells had very low F-actin formation at all focal planes. Arrows indicate representative phagocytic cups colocalized with SRBCs. Bar, 10 µm.

Impaired Fc{gamma}R Signal Initiation in hck-/-fgr-/-lyn-/- Macrophages.
As protein phosphorylation on tyrosine residues is essential for Fc{gamma}R-mediated phagocytosis to occur (28) and Src family members are believed to be crucial elements in propagation of the tyrosine kinase signal, we examined several important signaling reactions downstream of Fc{gamma}R activation in wild-type and hck-/-fgr-/-lyn-/- macrophages. After antibody-mediated Fc{gamma}R cross-linking, hck-/-fgr-/-lyn-/- macrophages showed dramatically reduced levels of overall tyrosine phosphorylation (Fig 4). Even at later time points (>20 min), when phagocytosis was clearly proceeding in hck-/-fgr-/-lyn-/- macrophages (Fig 1a and Fig B), very few changes in tyrosine phosphorylation were seen.



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Figure 4. Impaired Fc{gamma}R-induced tyrosine phosphorylation of cellular proteins in hck-/-fgr-/-lyn-/- macrophages. Cells were stimulated by incubation with anti-Fc{gamma}R mAb 2.4G2 followed by anti–rat IgG cross-linking for the indicated times. Cells were lysed, and equivalent amounts of cellular protein were subjected to SDS-PAGE and immunoblotting with antiphosphotyrosine antibodies.

Since initiation of Fc{gamma}RI and Fc{gamma}RIII signaling and phagocytosis is dependent on tyrosine phosphorylation of the accessory {gamma} chain, we examined this event in 32PO4-labeled macrophages stimulated with Fc{gamma}R cross-linking antibodies. As the {gamma} chain is phosphorylated on threonine and serine residues under resting conditions (29) (30), immunoprecipitation of the {gamma} chain from 32P-labeled wild-type or hck-/-fgr-/-lyn-/- macrophages lysates revealed the presence of a faster migrating phosphoband present at all time points examined (Fig 5 A). This band was not detected in anti-Igß immunoprecipitates from the same lysates (data not shown), suggesting that this protein is indeed the constitutively phosphorylated {gamma} chain. However, after Fc{gamma}R cross-linking for 15 min (Fig 5 A; and as early as 2 min, data not shown), a slower migrating protein appeared in the {gamma} chain immunoprecipitates from wild-type cells only. Since the induced phosphorylation of the {gamma} chain after Fc{epsilon}RI or Fc{gamma}R cross-linking is predominantly on tyrosine residues (29) (30), this species most likely represents newly tyrosine-phosphorylated {gamma} chain. In contrast to wild-type cells, no detectable induction of {gamma} chain phosphorylation was observed in stimulated hck-/-fgr-/-lyn-/- macrophages in four independent experiments.





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Figure 5. Impairment of proximal Fc{gamma}R-induced phosphorylation events in hck-/-fgr-/-lyn-/- macrophages. 32PO4-labeled (A) or unlabeled (B and C) macrophages were stimulated by incubation with anti-Fc{gamma}R mAb 2.4G2 followed by anti–rat IgG cross-linking for the indicated times. Cells were lysed, and equivalent amounts of cellular protein were subjected to immunoprecipitation with anti-{gamma} chain (A), anti-Syk (B), or anti-Cbl (C) antibodies. Each immunoprecipitation experiment was performed on lysates from an independent set of Fc{gamma}R-stimulated macrophages. After SDS-PAGE, phosphoproteins were detected either by autoradiography (A) or by immunoblotting (I.B.) with 4G10 antiphosphotyrosine antibodies (B and C). Membranes from B and C were stripped and reprobed with anti-Syk and anti-Cbl antibodies, respectively.

In the widely held model of immune receptor activation, it is proposed that the initial phosphorylation of ITAMs by Src family kinases allows for the binding and subsequent activation by phosphorylation of Syk. Therefore, we assessed the phosphotyrosine content of Syk in cells stimulated through the Fc{gamma}R (Fig 5 B). In wild-type macrophages, there was a rapid and sustained induction of Syk phosphorylation after Fc{gamma}R cross-linking, which began to decline only after 30 min of stimulation. The picture was quite different in hck-/-fgr-/-lyn-/- macrophages, as Syk phosphorylation was not detectable until cells had been stimulated for at least 20 min and the level of Syk phosphorylation achieved was much lower. The enzymatic activity of Syk was similarly compromised in Fc{gamma}R-stimulated hck-/-fgr-/-lyn-/- macrophages (13).

Activation of the PI 3-Kinase Pathway Is Impaired in hck-/-- fgr-/-lyn-/- Macrophages.
To correlate downstream signaling events with the delayed phagocytosis seen in mutant macrophages, we next assayed components of the PI 3-kinase pathway, known to have a role not only in phagocytosis (15) but also in membrane trafficking (31) and other cytoskeletal-induced events (32). Although it is not known precisely how Fc{gamma}Rs activate PI 3-kinase, this may involve Cbl, a multidomain adaptor protein that is one of the major tyrosine kinase substrates in signaling complexes formed after engagement of Fc{gamma}Rs on macrophages (33). Cbl binds to and recruits the p85 regulatory subunit of PI 3-kinase via a phosphotyrosine–SH2 domain interaction (34). In bone marrow–derived macrophages from wild-type mice, the phosphorylation of Cbl was detected very early after receptor cross-linking and the peak in this reaction strikingly paralleled that of Syk phosphorylation (Fig 5 C). In hck-/-fgr-/-lyn-/- macrophages, we observed both a delayed onset and diminished peak in Cbl phosphorylation, again parallel to that seen for Syk.

We next examined the formation of Cbl–PI 3-kinase complexes in Fc{gamma}R-stimulated macrophages by assessing the amount of lipid kinase activity found in Cbl immunoprecipitates. In wild-type macrophages stimulated through the Fc{gamma}R, we observed an eightfold increase in Cbl-associated PI 3-kinase activity, which peaked 5 min after receptor cross-linking (Fig 6 A). The activity detected in response to Fc{gamma}R cross-linking of hck-/-fgr-/-lyn-/- cells was greatly reduced, reaching only a twofold induction, and did not occur until 20 min after receptor cross-linking. In contrast, the amount of Cbl-associated PI 3-kinase activity in wild-type and hck-/-fgr-/-lyn-/- macrophages stimulated through the CSF-1 receptor was comparable (Fig 6 B), indicating that the mutant cells did not harbor any intrinsic defects in PI 3-kinase but rather that the reduced activation observed after Fc{gamma}R simulation was due to the paucity of tyrosine-phosphorylated Cbl with which p85 could associate (see Fig 5 C).



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Figure 6. Impaired Fc{gamma}R-, but not CSF-1R–induced signaling through the PI 3-kinase pathway in hck-/-fgr-/-lyn-/- macrophages. Cells were stimulated through the Fc{gamma}R as in the legend to Fig 3 (A and C) or by incubation with LCM (B and D), and cell lysates were immunoprecipitated (I.P.) with anti-Cbl (A and B) or antiphosphotyrosine (C and D, anti-pTyr) antibodies. Washed immunoprecipitates were then subjected to a lipid kinase assay using phosphatidylinositol as a substrate. The spots shown represent {gamma}-32P–labeled phosphatidylinositol 3-phosphate after TLC chromatography and autoradiography. Fold induction, shown below each lane, was quantitated using a PhosphorImager® and ImageQuant® software (Molecular Probes).

In addition to Cbl, other tyrosine-phosphorylated proteins may also interact with the p85 regulatory subunit of PI 3-kinase, affecting its subcellular location and activation (35) (36). Therefore, we also assayed PI 3-kinase activity in total antiphosphotyrosine immunoprecipitates from stimulated macrophages. The induction of phosphotyrosine-associated PI 3-kinase activity in Fc{gamma}R-stimulated cells was less than that seen associated with Cbl, but the PI 3-kinase activity in antiphosphotyrosine immunoprecipitates was similarly delayed and reduced in hck-/-fgr-/-lyn-/- macrophages relative to wild-type cells (Fig 6 C). However, in cells stimulated with CSF-1 via its receptor tyrosine kinase, the fold induction of phosphotyrosine-associated PI 3-kinase activity was high in both mutant and wild-type cells (Fig 6 D).

Activation of the Erk but Not JNK MAP Kinase Pathway Is Impaired in hck-/-fgr-/-lyn-/- Macrophages.
Activation of the MAP kinases Erk1 (p44) and Erk2 (p42) also occurs after Fc{gamma}R cross-linking in murine macrophages or human monocytes, although a specific role for this family of serine/threonine kinases in phagocytosis has not been determined (37) (38). We used a gel mobility shift assay to assess levels of phosphorylated, activated Erk1/2 in Fc{gamma}R-stimulated macrophages. After Fc{gamma}R cross-linking, Erk1/2 activation was observed within 5–10 min in wild-type cells, but only weakly after 20 min in hck-/-fgr-/-lyn-/- macrophages (Fig 7 A). These findings were also confirmed by in vitro kinase assays of Erk1/2 activity using the fusion protein GST-Elk1 as substrate (data not shown). In contrast, Erk1/2 activation occurred similarly in both wild-type and mutant cells after LPS stimulation ((20); Fig 7 B), indicating that there was not a general block in activation of this pathway in hck-/-fgr-/-lyn-/- cells.




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Figure 7. MAP kinase and JNK activation in hck-/-fgr-/-lyn-/- macrophages. Cells were stimulated by incubation with anti-Fc{gamma}R mAb 2.4G2 followed by anti–rat IgG cross-linking (A and C) or by incubation with 1 µg/ml LPS (B) for the indicated times. After cell lysis, equivalent amounts of protein were subjected to SDS-PAGE and immunoblotting with anti-Erk1/2 antibodies (A and B). For C, equivalent amounts of cellular lysates were subjected to immunoprecipitation with polyclonal anti-JNK antibodies followed by an in vitro kinase assay using a c-Jun GST fusion protein as a substrate. After SDS-PAGE and transfer to nitrocellulose, {gamma}-32P–labeled substrate was detected by autoradiography (top). The membrane was reprobed with an anti-JNK1 mAb to verify similar amounts of immunoprecipitated protein (bottom).

Recent reports have demonstrated a requirement for Rac1 and Cdc42 in Fc{gamma}R-mediated phagocytosis (39) and have implicated these GTPases in activation of the JNK pathway (40). Using a solid-phase in vitro kinase assay, we assayed for JNK activity in wild-type and hck-/-fgr-/-lyn-/- macrophages and observed activation of JNK beginning 20 min after Fc{gamma}R cross-linking (Fig 7 C). In sharp contrast to all other signaling reactions we studied, this response was similar in both wild-type and triple mutant macrophages. Probing of stimulated cell lysates with an antibody that specifically recognizes the phosphorylated, active form of JNK allowed for earlier detection of activated JNK (2–5 min), yet again no difference in the response of the two cell types to Fc{gamma}R cross-linking was noted (data not shown).

The Kinases Hck, Fgr, and Lyn Are Redundant for Phagocytosis but Not for Specific Signaling Events.
Although Hck, Fgr, and Lyn have all been shown to be receptor associated and activated upon Fc{gamma}R stimulation, the roles of these kinases may not be redundant (19) (41). To address this issue, we examined the rate of phagocytosis in bone marrow–derived macrophages from hck-/-fgr-/- double mutant or hck-/- and lyn-/- single mutant mice. All mutant macrophages displayed similar cell surface expression of Fc{gamma}RII and III as determined by flow cytometry (not shown). Likewise, cells from all three mutant mouse strains phagocytosed 51Cr-EAs with approximately the same kinetics as wild-type macrophages (Fig 8a and Fig b; and data not shown). Although clearly not a dramatic defect, it should be noted that in some experiments we did observe a slight delay in phagocytosis in lyn-/- macrophages (Fig 8 B).




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Figure 8. Normal phagocytosis in single and double mutant macrophages. Phagocytosis assays using 51Cr-EAs were performed as in the legend to Fig 1 with bone marrow–derived macrophages from hck-/-fgr-/- double mutant (A) or hck-/- and lyn-/- single mutant mice (B). Data shown are representative of four independent experiments on cells pooled from two to four mice per experiment. Two separate wild-type macrophage cultures are shown in B to illustrate normal variation between genetically identical samples.

Interestingly, when we assayed biochemical signaling reactions in single and double mutant macrophages, diverse roles for Hck, Fgr, and Lyn became apparent. In antiphosphotyrosine immunoblots of total cell lysates (Fig 9 A) or Syk immunoprecipitates (Fig 9 B), we detected a rapid and robust response in Fc{gamma}R–cross-linked wild-type and hck-/-fgr-/- macrophages. Thus, neither Hck nor Fgr is crucial for the initial phosphotyrosine response after Fc{gamma}R cross-linking. However, in macrophages lacking Lyn, there was a marked decline in the global phosphotyrosine response and a decrease in Syk phosphorylation, although not as complete as in cells lacking all three kinases. The reduced tyrosine phosphorylation observed in lyn-/- macrophages compared with hck-/-fgr-/- cells suggests that, of the three principal Src family kinases expressed in macrophages, Lyn plays the dominant role in Fc{gamma}R signal transduction, including initiation of Syk phosphorylation. However, lack of this kinase alone was not sufficient to physiologically impair Fc{gamma}R-mediated phagocytosis, at least under the conditions examined.




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Figure 9. Dominant role of Lyn in Fc{gamma}R-mediated signaling events. Cells were stimulated by incubation with anti-Fc{gamma}R mAb 2.4G2 followed by anti–rat IgG cross-linking for the indicated times. After cell lysis, equivalent amounts of cellular protein were subjected to SDS-PAGE and immunoblotting (I.B.) with antiphosphotyrosine antibodies (A) or to immunoprecipitation with anti-Syk antibodies followed by antiphosphotyrosine immunoblotting (B). The membrane in B was stripped and reprobed with anti-Syk antibodies to verify equivalent amounts of immunoprecipitated protein.


  Discussion
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
References

To examine the role of Src family kinases in Fc{gamma}R-mediated signaling and phagocytosis, we have analyzed these functions in macrophages derived from mice lacking the three predominant family members expressed in these cells, Hck, Fgr, and Lyn. Indicative of an important role for Src family kinases in Fc{gamma}R signaling, macrophages deficient in these kinases exhibited poor proximal ({gamma} chain and Syk tyrosine phosphorylation) and downstream (PI 3-kinase and MAP kinase activity) signaling reactions. Fc{gamma}R-mediated phagocytosis was also defective in triple mutant macrophages at early times after binding of antibody-coated erythrocytes, but by 20–30 min, substantial phagocytosis did occur. Likewise, Fc{gamma}R-mediated induction of respiratory burst was also dramatically reduced in triple mutant cells.

Src family kinases are thought to initiate signaling after clustering of multisubunit immune recognition receptors by phosphorylating tyrosines in the ITAM sequence of receptor cytoplasmic domains, thereby allowing for recruitment of Syk (or ZAP-70 in T cells) to the phosphorylated ITAMs (5) (6) (42). Consistent with this view, we did not detect any inducible FcR {gamma} chain phosphorylation in hck-/-fgr-/-lyn-/- macrophages. Moreover, both the phosphorylation and enzymatic activity of Syk after Fc{gamma}R engagement were markedly reduced in the triple mutant macrophages. Eventually a small degree of Syk tyrosine phosphorylation did occur, and the onset of this phosphorylation correlated well with the initiation of significant phagocytosis in these cells. The low amount of induced Syk phosphorylation may be important for the observed phagocytosis that did occur in triple mutant cells. It is possible that clustered Fc{gamma}Rs can recruit and activate Syk kinase in the absence of ITAM phosphorylation by Src family kinases, albeit inefficiently. A precedent for this hypothesis comes from experiments with chimeric transmembrane proteins bearing Syk intracellularly. These chimeric receptors can trigger phagocytosis and other immune cell functions in the absence of Src family kinase activation (43) (44) (45). Syk, in contrast to ZAP-70, can directly activate itself by phosphorylation (46) (47), and this autoactivation could allow for initiation of downstream reactions that lead to phagocytosis.

Comparing the phenotypes of hck-/-fgr-/-lyn-/- and syk-/- macrophages reveals that these cells have very different defects in phagocytosis. In syk-/- cells, the formation of actin cups surrounding EAs was apparently normal (13), whereas in the hck-/-fgr-/-lyn-/- macrophages this process was clearly delayed. Conversely, in syk-/- cells, closure of the membrane to allow for internalization of particles did not occur, whereas successful phagocytosis was achieved by hck-/-fgr-/-lyn-/- macrophages. Taken together, these observations indicate that Src family and Syk kinases have distinct functions in the propagation of a phagocytic signal through the Fc{gamma}R. Rather than there being a linear flow of information from the receptor to Src family kinases and {gamma} chain phosphorylation, to Syk recruitment/activation, and on to downstream events, there appears to be a bifurcation whereby the Src family kinases act both to direct actin polymerization into phagocytic cups and to stimulate Syk (Fig 10). The critical role of Syk is in the subsequent closing of the phagosome to give full internalization. Since hck-/-fgr-/-lyn-/- macrophages do form some actin cups, it is likely that another tyrosine kinase can take over this function. Syk is a good candidate for this alternative kinase, since clustering of chimeric transmembrane proteins bearing Syk intracellularly in COS cells effectively triggered redistribution of F-actin as well as phagocytosis (43). Similarly, actin assembly triggered by CD16–{gamma} chain fusion proteins was found to depend on Syk (48). The autoactivation of Syk would likely be inefficient, especially initially, and hence is consistent with the delayed onset of phagocytosis observed in triple mutant cells. Thus, although the Src family members contribute significantly to the rate of Fc{gamma}R-mediated phagocytosis, they are in fact dispensable.



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Figure 10. Model for the role of Src family kinases in Fc{gamma}R-mediated phagocytosis. Src family kinases are responsible for phosphorylation of the FcR {gamma} chain, recruitment of Syk to the receptor, and activation of Syk, which then mediates signaling leading to internalization of the bound particle. The results presented here, however, demonstrate an additional function of Src family kinases: to promote actin polymerization and phagocytic cup formation, as evidenced by the significant delay in these events in hck-/-fgr-/-lyn-/- macrophages and their normal kinetics in Syk-deficient macrophages. Other signaling events possibly involving Syk (indicated by the broken arrows) include ITAM phosphorylation, stimulation of slow actin cup formation, and recruitment and/or autoactivation. These additional signaling reactions may account for residual phagocytosis in the absence of the Src family kinases.

As noted above, Src family kinases are thought to be responsible for phosphorylation of ITAM sequences in lymphocyte immune recognition receptors and can phosphorylate the ITAM in Fc{gamma}RIIa in vitro (49). Although we failed to observed {gamma} chain phosphorylation in hck-/-fgr-/-lyn-/- cells, {gamma} chain phosphorylation also does not occur normally in syk-/- macrophages after Fc{gamma}R ligation (14). The lack of {gamma} chain phosphorylation in syk-/- cells may be due to the fact that Syk is able to protect the phosphorylated ITAMs from phosphatases by binding to them. Alternatively, Syk may directly amplify ITAM phosphorylation after recruitment to the receptor complex and activation by Src family kinases. Thus, comparison of these two knockout cell types does not definitively determine which kinases are responsible for ITAM phosphorylation.

We cannot rule out the possibility that the slow activation of signaling and phagocytosis in hck-/-fgr-/-lyn-/- macrophages is accomplished by another Src-related kinase. However, current evidence does not favor this possibility. No compensatory increase in expression of other known Src family kinases has been detected in these cells (20), although very low levels of Src appear to be expressed in the cultured macrophages used in these experiments. By breeding the triple mutant mice to Src mutant mice, we have recently generated quadruple mutant src-/-hck-/-fgr-/-lyn-/- mice; macrophages from these mice exhibit phagocytosis at rates and levels similar to those seen in the triple mutant macrophages (data not shown). Moreover, treatment of cells with the tyrosine kinase inhibitor PP1, which inhibits Src family but not Syk kinases (50), also did not completely block EA phagocytosis (data not shown). Together, these observations suggest that Fc{gamma}R phagocytosis is not completely dependent on Src family kinases.

The possibility of functional redundancy between the Src family kinases in Fc{gamma}R signaling and phagocytosis was examined using macrophages isolated from single or double mutant mice. Interestingly, macrophages lacking Hck and Fgr exhibited normal Fc{gamma}R signaling and phagocytosis, suggesting that neither of these two kinases is crucial for the initial response to Fc{gamma}R cross-linking. In contrast, macrophages lacking Lyn showed a significant defect in signaling, while phagocytosis in these mutant macrophages occurred at a normal, or near normal rate. Apparently phagocytosis requires only a low level of Fc{gamma}R signaling. Thus, Fc{gamma}R signaling reactions were largely dependent on Lyn, whereas some residual function could be supplied by Hck and/or Fgr. For Fc{gamma}R-mediated phagocytosis, however, there was functional redundancy between the different Src family members.

The signaling events downstream of Syk activation that are important for Fc{gamma}R-mediated phagocytosis are still not well understood, but a leading candidate is PI 3-kinase activation. Recruitment of PI 3-kinase to the membrane may be mediated by association with Cbl, which becomes tyrosine phosphorylated after Fc{gamma}R cross-linking and is translocated to the membrane where the phosphoinositide substrates of PI 3-kinase are located (33). Since Fc{gamma}R-induced Cbl phosphorylation and phosphotyrosine- and Cbl-associated PI 3-kinase activities were diminished in hck-/-fgr-/-lyn-/- macrophages, it is likely that decreased amounts of D3 phosphoinositides were formed in the membrane, which may contribute to the reduced phagocytosis in these cells. In contrast, it appears that activation of Erk1 and Erk2 is not required for Fc{gamma}R phagocytosis, since we were unable to detect significant activation of these kinases in hck-/-fgr-/-lyn-/- macrophages even at time points when these cells showed some phagocytosis. Similarly, Karimi and Lennartz (37) concluded that although MAP kinase activation occurs during IgG-mediated phagocytosis in human monocytes, it is not required, since treatment of cells with a selective inhibitor of this pathway blocked Erk2 activation without affecting phagocytosis. Taken together, these results suggest that MAP kinase activity does not play a central role in signaling to cytoskeletal rearrangements necessary for IgG-mediated phagocytosis, but rather may be involved in signaling to other Fc{gamma}R-mediated events in leukocytes, such as regulation of transcription.

In summary, this report describes a genetic approach to dissect the signaling pathways emanating from the Fc{gamma}R. Our findings indicate that Src family tyrosine kinases play an important role in directing polymerization of actin adjacent to signaling receptors. Src family tyrosine kinases also greatly promote the activation of the Syk tyrosine kinase, which is essential for completion of phagocytosis. In macrophages lacking detectable Src family kinases, phagocytosis, respiratory burst, actin cup formation, Syk activation, and activation of downstream signaling events were all delayed and decreased to some extent, but not abolished entirely, demonstrating an important although nonessential role of these kinases in Fc{gamma}R-mediated functional responses.


  Footnotes

C.J. Fitzer-Attas' current address is Bio-Technology General Ltd., Kiryat Weizmann, Rehovot 76326, Israel. M.T. Crowley's current address is Scripps Institute, La Jolla, CA 92093. F. Meng's current address is Telik Corporation, South San Francisco, CA 94080.
1 Abbreviations used in this paper: EA, IgG-coated sheep erythrocyte; Erk, extracellular signal–regulated kinase; GST, glutathione S-transferase; ITAM, immunoreceptor tyrosine-based activation motif; JNK, c-Jun NH2-terminal kinase; LCM, L cell–conditioned medium; MAP, mitogen-activated protein; PI 3-kinase, phosphatidylinositol 3-kinase; SH2, Src homology 2.


  Acknowledgements
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
References

The authors would like to thank Dr. Michele Lennartz for invaluable advice on phagocytosis assays, Dr. J.P. Kinet for anti-{gamma} chain antibodies, Larry Lem for excellent assistance with photomicroscopy, and Drs. E. Brown, M. MacKichan, and S. Harmer for their constructive comments.

This work was supported by National Institutes of Health grants to C.A. Lowell (DK50267 and HL54476) and A.L. DeFranco (AI20038). F. Meng was supported by National Institutes of Health training grant DK07636.

Submitted: 30 September 1999
Revised: 20 December 1999
Accepted: 22 December 1999


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Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
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