By
From the * Transplantation and Immunobiology Group, The T cell receptor (TCR) is a versatile receptor able to generate different signals that result in
distinct T cell responses. The pattern of early signals is determined by the TCR binding kinetics that control the ability of the ligand to coengage TCR and coreceptor. Coengagement of
TCR and CD4 results in an agonist signaling pattern with complete tyrosine phosphorylation
of TCR subunits, and recruitment and activation of ZAP-70. In contrast, TCR engagement
without CD4 coengagement causes a partial agonist type of signaling, characterized by distinct
phosphorylation of TCR subunits and recruitment but no activation of ZAP-70. The pathways
triggered by partial agonist signaling are unknown. Here, we show that agonists cause association of active lck and active ZAP-70 with p120-GTPase-activating protein (p120-GAP). These
associations follow engagement of CD4 or CD3, respectively. In contrast, partial agonists do
not activate lck or ZAP-70, but induce association of p120-GAP with inactive ZAP-70. Despite these differences, both agonist and partial agonist signals activate the mitogen-activated protein kinase (MAPK) pathway. However, MAPK activation by partial agonists is transient,
supporting a kinetic, CD4-dependent model for the mechanism of action of variant TCR
ligands. Transient MAPK activation may explain some of the responses to TCR partial agonists
and antagonists.
The current paradigm of T cell activation proposes that
signal transduction from the TCR is initiated after
ligand binding by src-kinase-dependent phosphorylation of
immune receptor, tyrosine- based activation motifs (ITAMs)1
in the cytoplasmic domains of the CD3/TCR Implicit in this paradigm is the assumption that similar
levels of TCR occupancy by different ligands will lead to
identical signal transduction events. However, recent evidence indicates that the TCR is not an off/on signal transduction complex, but rather is able to transduce different
patterns of signals that can cause dramatic effects on T cell
function (7, 8). These distinct patterns of early TCR-mediated signaling are induced by variant TCR ligands that
have conservative substitutions in the TCR contact points
of the peptide or the MHC molecule (9, 10). As a result,
they act as partial agonist or antagonist ligands of the TCR
depending on whether they cause partial activation of the
T cell or they inhibit agonist-induced activation (for review see references 11, 12). The differential T cell activation induced by these variant TCR ligands correlates with
a distinct pattern of early TCR-mediated signaling that is
characterized by predominant appearance of the p21 form
of phospho- It is not known how the partial agonist type of early
TCR-mediated signaling is transmitted to downstream
pathways. Two general possibilities can be considered. One
is that this differential signaling may transduce distinct or
diminished signals along each one of the pathways emanating from the TCR, resulting in less signal being delivered
to the nucleus. Alternatively, the early TCR-mediated signaling in partial agonist mode could translate into dissociated delivery of signals, such as only some of the pathways
emanating from the TCR are activated whereas others are not. Here, we explore this issue in relation to one of the
pathways emanating from the TCR. Some of the functional responses induced by TCR partial agonist ligands,
such as TCR downmodulation, can be attributed to serine
phosphorylation (21). Therefore, we examined the effects of TCR partial agonists on the Ras-MAPK pathway of signal transduction, because it primarily involves serine/ threonine kinases and regulates cell proliferation and differentiation (24). Here, we show that signaling from the TCR
in a partial agonist mode causes recruitment of p120-GTPase-activating protein (p120-GAP), a potential Ras effector
molecule, to the receptor, and is capable of activating the
extracellular regulated kinase (ERK)-1 and ERK-2 MAPKs.
However, it does so in a more transient fashion than full
agonist ligands of the TCR do. Our results provide the first
experimental evidence for activation of the MAPK after partial agonist signaling from the TCR. Activation of the
Ras-MAPK pathway may explain some of the effects seen
after TCR engagement with these variant ligands, such as
downmodulation of TCR or split cytokine production
without proliferation.
Cells.
Departments of Microbiology and Immunology and Medicine,
Abstract
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References
Introduction
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References
chains (for
review see reference 1). Phosphorylation of the ITAMs
leads to recruitment and activation of
-associated protein
(ZAP)-70 through its SH2 domain, and subsequent recruitment and activation of other proteins such as p36 (linker
for activation of T cells) (LAT), vav, and SLP-76, through
SH2-SH3 domain interactions. This leads to the formation
of multimolecular complexes that activate several signaling cascades such as the phospholipase C-
1 (PLC-
1)-dependent cascade (2), and the ones emanating from activation of
p21ras such as the mitogen-activated protein kinase
(MAPK) pathway and the phosphatidyl-inositol 3-kinase
(PI-3K) pathway (3). These signaling cascades ultimately
converge on the nucleus resulting in the changes of gene
expression that characterize T cell activation.
with little phosphorylation of CD3-
, and
with recruitment of ZAP-70 to the TCR-activating complex but no phosphorylation nor activation of this protein
tyrosine kinase (13, 14). This distinct pattern of TCR-mediated signal results in an array of intracellular changes that include increase in cell size (10), induction of acidification of the extracellular environment (15, 16), transient or
partial calcium signals (17, 18), modulation of TCR expression (19), and upregulation of some cell surface molecules
such as IL-2 receptor and leukocyte function-associated
antigen 1 (7, 8, 12, 20). These cellular changes translate
functionally into inhibition of agonist-induced cell proliferation and/or cytokine production, split cytokine production profiles, and/or induction of T cell anergy. Together,
this evidence strongly suggests that the distinct pattern of
TCR-mediated signaling induced by partial agonist ligands can activate, at least, some signaling pathways emanating
from the TCR.
Materials and Methods
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References
and E
chains of I-Ek, as well as the costimulatory
molecules CD54 (ICAM-1) and CD80 (B7-1), were used as APCs
(14, 26, 27).
Reagents.
The following peptide was used for these experiments: PCC(81-104) (IFAGIKKKAERADLIAYLKQATAK). This peptide was commercially provided by Procyon Biopharma Inc. (London, Ontario, Canada) and purified to >85% by HPLC before use. Pervanadate was prepared by dissolving 100 µl of 0.1 M sodium orthovanadate (Sigma Chemical Co., St. Louis, MO) into 900 µl of double distilled water and 3.3 µl of 30% hydrogen peroxide, and kept at room temperature for 15 min before use.Monoclonal Antibodies.
4G10 (provided by Dr. B. Druker, Oregon Health Sciences University, Portland, OR) is a mouse IgG2b monoclonal antibody against phosphotyrosine, B4F8 is a mouse IgG2a monoclonal antibody against p120-GTPase activating protein (p120-GAP; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit immune sera against lck and ZAP-70 were provided by L.E. Samelson (CBMB, NICHD, National Institutes of Health, Bethesda, MD), and H57-597 is a hamster IgG against a common epitope of the TCRT Cell Stimulation.
Ag stimulation of T cells was done as previously described (14, 33). In brief, P13.9 cells (106 cells/group) were plated in 24-well plates with or without PCC(81-104), and incubated at 37°C, overnight. The next day, T cells (107 cells/ group) were added. Plates were then spun down (1,000 rpm for 30 s) and incubated at 37°C for 10 min. T cell stimulation with heterofunctional antibodies was performed in a final volume of 100 µl (107 cells/group) in Eppendorf tubes with the indicated heterofunctional antibodies (10 µg/ml) or different amounts of pervanadate for 10 min at 37°C. After stimulation, T cells were harvested in cold PBS containing sodium orthovanadate (400 µM) and EDTA (400 µM), and lysed in 1× lysis buffer (1% Triton X-100, 150 mM NaCl, 10 mM Tris, pH 7.6, 5 mM EDTA, 1 mM sodium orthovanadate, 10 µg/ml leupeptin, 10 µg/ml aprotinin, and 25 µM p-nitrophenyl-p'-guanidino-benzoate).Tyrosine Phosphorylation Analysis.
Detection of tyrosine phosphorylation of proteins in ZAP-70 or p120-GAP immunoprecipitates was performed as described (14). 4G10 monoclonal antibody was used for immunoblotting. Rabbit antiserum to ZAP-70 was used to immunoprecipitate ZAP-70 and associated proteins, and mouse monoclonal antibody against p120-GAP was used for immunoprecipitation of p120-GAP. Immunoprecipitations were performed from 107 cells/group (14). Signal detection was performed by chemiluminescence (Boehringer Mannheim, Laval, Quebec, Canada) and intensity was quantitated using an imaging densitometer (model GS 700; BioRad Labs., Hercules, CA) and the Molecular Analyst® software (version 1.0, BioRad Labs.). Detection of activated ERK-1 and ERK-2 was performed by Western blotting of cell lysates with the anti-ACTIVE MAPK (Promega) rabbit antiserum. In brief, 107 cells/sample was stimulated with the appropriate antibodies at different concentrations or for different times at 37°C. Next, cells were washed once with PBS containing sodium orthovanadate, and subsequently lysed in 1× lysis buffer (500 µl) on ice for 30 min. Lysates cleared of nuclear debris by centrifugation at 14,000 rpm at 4°C for 10 min, and 300 µl of supernatant was mixed with 4× sample buffer. 30 µl of this solution (4.5 × 105cell equivalents/group) were run in 12% SDS-PAGE, transferred to polyvinylidene difluoride membranes, and blotted with anti-ACTIVE MAPK antiserum (Promega) using chemiluminescence (Boehringer Mannheim).In Vitro Immune Complex Kinase Assays.
T cells (107/sample) were stimulated with either the anti-CD3-CD4 fos-jun antibodies, the anti-CD3 fos antibodies, or the anti-CD4 jun antibodies for 10-12 min at 37°C, mixing gently on a thermomixer. Cells were lysed with 1× lysis buffer for 30 min on ice. In vitro immune complex kinase assays of lck, ZAP-70, and p120-GAP immunoprecipitates were performed as previously described (14). MAPK activity assay was performed according to manufacturer specifications (New England Biolabs, Boston, MA). In brief, collected cell lysates were immunoprecipitated with a phospho-specific p42/p44 MAPK monoclonal antibody on protein A-Sepharose beads with gentle rocking, overnight at 4°C. The next day, the bead pellets were washed twice with 1× lysis buffer and twice with 1× kinase buffer (25 mM Tris, pH 7.5, 5 mMFlow Cytometric Analysis of TCR Downmodulation.
3C6 T cells were incubated with different concentrations of heterofunctional antibodies for 1-3 h at 37°C. Subsequently, T cells were harvested and stained with FITC-labeled monoclonal antibody against mouse TCR- ![]() |
Results |
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As we have previously reported
(33, 34), coengagement of CD3 and CD4 induces an agonist pattern of early signaling from the TCR. This pattern
is characterized by the presence of two forms of phosphorylated TCR- (p21 and p23), phosphorylation of CD3-
,
and recruitment and phosphorylation of ZAP-70 in phosphotyrosine blots of ZAP-70 immunoprecipitates (Fig. 1).
In contrast, phosphotyrosine blots of ZAP-70 immunoprecipitates from T cells stimulated by bivalent engagement of
CD3 show a partial agonist pattern of tyrosine phosphorylation. This is identified by predominant appearance of the
p21 form of phospho-
, with little or no phospho-
, and recruitment but no phosphorylation of ZAP-70 tyrosine
kinase. In previous studies (14, 33), we have shown that the
two patterns of early TCR-mediated signaling are not the
result of quantitative differences in TCR engagement because they do not overlap after titration of antigenic peptide or stimulating antibody. Bivalent engagement of CD4
alone did not induce significant tyrosine phosphorylation of
TCR subunits in ZAP-70 immunoprecipitates.
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Further qualitative differences between the agonist pattern and the partial agonist pattern of early TCR-mediated
signaling were seen when the kinase activity associated
with the TCR complex under conditions of bivalent CD3
engagement or after CD3 and CD4 coengagement were
examined. As genetic and biochemical approaches have
previously demonstrated (36, 37), TCR-mediated signaling involves recruitment and activation of lck and ZAP-70 tyrosine kinases. Consistent with this, we found increased kinase activity associated with lck in T cells activated in an
agonist pattern after CD3 and CD4 coengagement (Fig. 2).
In these conditions, immune complex kinase assays after lck
immunoprecipitation showed increased autophosphorylation of lck and phosphorylation of a 70-kD band as well as
two bands of 23 and 32 kD, bands compatible with ZAP-70, TCR-, and CD3-
, respectively (Fig. 2). ZAP-70
immunoprecipitates showed significant autophosphorylation of ZAP-70 (Fig. 2). In contrast, no significant increase
of lck phosphorylation or kinase activity was detected in lck
immunoprecipitates or ZAP-70 immunoprecipitates from
cell lysates of T cells activated in a partial agonist mode after bivalent CD3 engagement alone (Fig. 2). From these
experiments, we concluded that bivalent engagement of
CD3 and coengagement of CD3-CD4 fully reproduce the
distinct patterns of early TCR signaling seen after engagement of TCR with ligands with partial agonist or agonist
properties, respectively (13, 14).
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Despite these differences in early TCR-mediated signaling, we found that both agonist ligand and partial agonist ligand-induced similar loss of surface TCR expression as measured by flow cytometric analysis using a monoclonal antibody against mouse TCR (H57-597; Fig. 3). This finding implies that some signals from the TCR are equally induced by both types of ligands since TCR downmodulation is a reflection of T cell activation (19, 38) and protein kinase C-dependent serine phosphorylation of CD3 chains (23, 39).
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To explore the downstream effects of these two qualitatively distinct patterns of early signaling, we initially looked at recruitment and activation of different molecules involved in the Ras-MAPK pathway of signal transduction. Among the many Ras effector molecules, we concentrated on p120-GAP for three reasons. First, Ag-induced T cell activation induces tyrosine phosphorylation of p120-GAP (44). Second, p120-GAP regulates Ras function by promoting GTPase activity (45). And third, p120-GAP may be itself a Ras effector molecule (48). We performed p120-GAP immunoprecipitates and examined phosphorylation and association of p120-GAP after agonist and partial agonist conditions of stimulation. T cell activation in an agonist mode (either by peptide-MHC molecule complexes on the surface of an APC or by heterofunctional antibodies against CD3 and CD4) induced a rapid, dose-dependent increase in phosphotyrosine content of p120-GAP (Fig. 4). In contrast, partial agonist signaling from the TCR did not induce tyrosine phosphorylation of p120-GAP. In addition, bivalent CD4 engagement alone did not induce tyrosine phosphorylation of p120-GAP either. Despite the differences in p120-GAP tyrosine phosphorylation, p120-GAP immunoprecipitation showed a tyrosine-phosphorylated 70-kD band that was immunoreactive with an antiserum against ZAP-70 in T cells stimulated in full agonist mode (Fig. 5, a and b). Surprisingly, this 70-kD protein was also associated to p120-GAP in T cells stimulated in partial agonist mode (Fig. 5 b), although it was not phosphorylated (Fig. 5 a), indicating that either pattern of early TCR-mediated signaling can induce p120-GAP recruitment to ZAP-70, but only agonists can induce its phosphorylation.
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To further support that this 70-kD band was ZAP-70, the protein was depleted from the lysate by sequential rounds of immunoprecipitation with an antiserum against ZAP-70 (Fig. 6). Interestingly, the data suggest that the proportion of ZAP-70 that is associated with p120-GAP is relatively low compared with the total amount of ZAP-70 in the cells. Reblotting of the same membrane with an antiphosphotyrosine antibody showed a similar result for phospho-ZAP-70. In addition, in vitro immune complex kinase assays on p120-GAP immunoprecipitates showed phosphorylated lck and ZAP-70 bands as well as phosphorylation of the cdb3 exogenous substrate for ZAP-70 after CD3-CD4 coengagement (Fig. 7). In contrast, and consistent with our previous findings on kinase assays of ZAP and lck immunoprecipitates from partial agonist-stimulated T cells, no kinase activity was detected in p120-GAP immunoprecipitates from these cells. The association between ZAP-70 and p120-GAP is unlikely due to coassociation of these molecules through TCR subunits because these proteins were not detected in p120-GAP immunoprecipitates. In these kinase assays, p120-GAP was tyrosine phosphorylated only in the immunoprecipitates from T cells stimulated by agonist ligands.
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Previous reports have demonstrated that p120-GAP can associate with lck and is a substrate of this src-kinase (49, 50). Given that lck is noncovalently associated with CD4 (51), the lack of coengagement of CD3 and CD4 might translate into lack of association between p120-GAP and lck and could explain the lack of tyrosine phosphorylation of p120-GAP. To test this hypothesis, we examined whether the two patterns of early TCR-mediated signaling differed in the ability of p120-GAP to associate with lck. As shown in Fig. 5 c, there is no association between p120-GAP and lck under basal conditions. However, after TCR-mediated signaling by the agonist, there was significant association of these two molecules. Both the p56 and the activation-dependent p59 form of lck were present in p120-GAP immunoprecipitates of T cells stimulated by coengagement of CD3 and CD4 confirming that T cell activation had occurred. Partial agonist type of TCR-mediated signaling resulted in no association of these two molecules. Of note was the observation that bivalent engagement of CD4 alone did induce the association of p120-GAP with lck as well as tyrosine phosphorylation of p120-GAP in immune complex kinase assays (Fig. 7); however, under these conditions only the resting p56 form of lck was detected.
Thus, our results indicate that p120-GAP is recruited and associated to ZAP-70 after engagement of the TCR in vivo. Early TCR signaling in agonist mode induces kinase activity of lck and ZAP and that correlates with tyrosine phosphorylation of the recruited p120-GAP. In contrast, early TCR-mediated signaling in partial agonist mode fails to induce kinase activity of lck and ZAP-70, and therefore it is not able to induce phosphorylation of the recruited p120-GAP.
Activation of ERK-1 and ERK-2 upon Early TCR-mediated Signaling in Agonist and Partial Agonist Modes.Activation of p21ras leads to recruitment and activation of Raf-1, which then activates MEK-1 and MEK-2, which subsequently activate ERK-1 and ERK-2 (24). Thus, we assessed the activation of these stages of the Ras-MAPK pathway by looking at the levels of double phosphorylated ERK-1 and ERK-2, as these are the active forms of ERK (24), and at the kinase activity of ERK-1 and ERK-2 measured as serine phosphorylation of exogenous Elk-1 as substrate. Immunoblotting with a monoclonal antibody against dual phosphorylated ERK-1 (p44) and ERK-2 (p42) showed a significant increase in these forms upon short stimulation with either anti-CD3-fos or with anti-CD3-fos-anti-CD4-jun (Fig. 8 a). ERK-1 and ERK-2 activation remained high for at least 10 min in T cells stimulated by the agonist ligand as well as in those stimulated by the partial agonist ligand. However, upon CD3 and CD4 coengagement, ERK-1 and ERK-2 activation did not start to significantly decrease until after 30 min and was still detectable after 60 min of stimulation. In contrast, ERKs activation decreased more rapidly after partial agonist signaling, with significantly decreased levels by 10 min after activation with bivalent CD3 engagement and disappearance after 30 min. The possibility of differential activation of p42 or p44 at the initial time points is unlikely given that the signal ratio for p44/p42 is similar for both groups. As ERK activation decreases, the loss of p44 phosphorylation occurs faster than the loss of p42 phosphorylation in both experimental groups.
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These results were confirmed using in vitro kinase assays for the ERKs (Fig. 8 b). Coengagement of CD3 and CD4 for 2 min induced a significant increase in kinase activity of ERKs as indicated by increasing serine phosphorylation of exogenous Elk-1 (Fig. 8 b). Under these conditions, ERK kinase activity remained high for up to 60 min. Bivalent engagement of CD3 alone also induced a substantial increase in phosphorylation at 2 min, which was equal to that observed after full agonist TCR-mediated signaling. Therefore, the initiation of ERK activation is similar after either agonist or partial agonist engagement of the TCR. However, the kinetics of phosphorylation and phosphoprotein decay of this pathway are distinctly different, with significant loss of kinase activity after 10 min and almost complete loss of activity after 30 min under conditions of partial agonist signaling from the TCR. The increase in ERK kinase activity seen after TCR-mediated signaling in agonist mode or partial agonist mode were both dose dependent (Fig. 8 c).
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Discussion |
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Variant TCR ligands with partial agonist properties induce a pattern of early TCR-mediated signaling that is distinct from the signals induced by agonist ligands (for reviews see references 12, 20). Although agonist ligands
induce appearance of two forms of phospho-TCR- (p21
and p23), phospho-CD3-
, and subsequent recruitment
and activation of ZAP-70, partial agonists of the TCR only
induce the p21 form of phospho-TCR-
and fail to activate recruited ZAP-70. This distinct signaling pattern correlates with restricted T cell responses such as limited cytokine production or induction of T cell anergy (7, 8).
Characterization of the steps linking the initial events after
receptor engagement with the downstream effector responses is important to understand T cell differentiation after TCR ligation. The results presented in this paper demonstrate that stimulation of T cells in a partial agonist mode
after bivalent engagement of CD3 causes recruitment and
association of p120-GAP with ZAP-70, and activation of
the Ras-MAPK pathway. However, in contrast to full agonist pattern of TCR-mediated signaling, partial agonist
TCR-mediated signaling activates ERK-1 and ERK-2
only transiently, either because of lack of sustained stimulation and normal decay or because of faster inactivation of
these serine/threonine kinases.
We have shown that p120-GAP can associate with ZAP-70 in vivo after early TCR-mediated signaling in agonist mode and in partial agonist mode. However, only full agonist type of signaling induced phosphorylation of p120-GAP. This is expected since only agonist ligands induce activation of lck and of ZAP-70 (13, 14), and lck can act as a kinase on ZAP-70 (36) and on p120-GAP (50). On the other hand, the partial agonist pattern of TCR-mediated signaling failed to activate the kinase activity of lck and did not induce association of p120-GAP to this src-kinase. This could explain the lack of tyrosine phosphorylation of ZAP-70 and of p120-GAP (34). The dissociated recruitment and activation of signaling molecules after partial agonist signaling from the TCR may have profound functional consequences. This is well established for ZAP-70, for which recruitment and activation are two separate, discrete events, and for which tyrosine phosphorylation correlates with kinase activity (14, 36, 52, 53). The biological implications of tyrosine phosphorylation of p120-GAP are still unknown.
What is the molecular basis for the association between
p120-GAP and these kinases? In the agonist type of signaling, both lck and ZAP-70 are tyrosine phosphorylated and
active. Given that p120-GAP has two SH2 domains (48),
the association of p120-GAP to these molecules can be explained on the basis of SH2-mediated interactions with
phospho-tyrosine-based motifs, as previously reported (54).
However, the molecular mechanism by which the ZAP-70-p120-GAP association occurs after early TCR signaling
in partial agonist mode is not clear because, in these conditions, ZAP-70 is not phosphorylated, excluding SH2-mediated interactions between these two molecules. Two
possible mechanisms can be proposed. One is that p120-GAP associates with ZAP-70 through an interaction involving the SH3 domain of p120-GAP and a proline-rich
region in ZAP-70. Close examination of the ZAP-70 sequence shows that it contains a PTLPAHP stretch between
residues 267 and 274, downstream of the SH2(C) domain
(52). This sequence conforms to the consensus sequence
for SH3 domain binding (55). This claim is further supported by the similarity between this sequence and the proline-rich sequence in G3BP (PQRPQR) that likely mediates the high affinity binding between this protein and the
SH3 domain of p120-GAP (56). The PTLPAHP sequence
only contains relatively minor substitutions between the
proline residues. This mechanism would be similar to that
reported for MAPK recruitment to the TCR via SH3 interactions with lck (57). Alternatively, the association between ZAP-70 and p120-GAP may occur through an intermediate molecule to which both bind through their
SH2 domains. One potential candidate could be a TCR
subunit such as TCR- or a CD3 chain because these are phosphorylated in tyrosines after partial agonist type of signaling from the TCR, and we know that this is sufficient
for ZAP-70 recruitment (14). However, we have failed to
detect the presence of TCR subunits by direct blotting of
p120-GAP immunoprecipitates as well as by phosphotyrosine blotting of these samples. Another candidate could
be p62-Sam68 (58, 59), although we do not have any indication of a tyrosine phosphorylated protein of this range in
p120-GAP immunoprecipitates.
How does activation of ERK-1 and ERK-2 occur after TCR-mediated signaling in partial agonist mode? Our results indicate that coengagement of CD4 with CD3 is not necessary for activation of the Ras-MAPK pathway, but it is required for optimal and sustained activation of this pathway (60). Coengagement of CD3 and CD4 may be especially important when the affinity of the TCR for its ligand is low, such as in the case of weak agonist ligands (61) or partial agonists and antagonists of the TCR (62, 63). Under these conditions, CD4 coengagement may increase the stability of TCR-peptide-MHC molecule complex engagement and provide sustained TCR-induced signaling (64). Our finding of transient activation of MAPKs in the absence of CD3-CD4 coengagement is therefore compatible with our previous model in which the efficiency of TCR- CD4 coengagement determines the quality of TCR-mediated signaling and partial agonism (33, 34). The transient kinetics of MAPK activation after partial agonist stimulation supports a CD4-dependent kinetic basis for differential TCR-mediated signaling by partial agonist ligands. TCR antagonism may represent conditions of a much lower range of TCR affinity that correlate with very transient or undetectable ERK activation (65).
It is important to note that bivalent engagement of CD3 may be a critical feature in the induction of partial agonist signaling in our experimental model, in line with recent claims that oligomerization of the TCR may be needed for initiation of signaling (66). The specific CD3 dimerization induced by our chimeric antibodies, in contrast to antibody-induced cross-linking (59), could explain the differences between our data showing lck-independent activation of ERKs and previous reports claiming that ERK activation is primarily lck dependent (67, 68). Some ligands, including the chimeric molecules used here, may induce receptor dimerization with the proper architecture to cause PKC-dependent activation of Raf-1 and ERK (69) in the absence of lck activation (68, 73). In vivo data would support this model. For example, although lck-deficient mice have a drastic reduction in the total number of double positive thymocytes, they still have a significant proportion of these cells (74). This would support a lck-independent pathway for MAPK activation given that differentiation into CD4+CD8+ thymocytes is critically dependent on activation of the MAPK pathway (75).
Based on the above mentioned considerations, we propose that partial agonist-induced differential phosphorylation of TCR subunits causes activation of Ras. This could be the result of activation of a kinase other than lck (possibly fyn; reference 78), which would concomitantly recruit ZAP-70 and activate some other signaling molecules like p95vav that shares homology with some guanine nucleotide releasing factors involved in Ras activation (79, 80). Ras activation would then recruit different effector molecules to the cell membrane, including Raf (81) and p120-GAP. Activation of Ras and Raf-1 would then result in activation of ERK-1 and ERK-2. In addition, the presence of p120-GAP in proximity to engaged and differentially phosphorylated TCR would favor the association of p120-GAP with recruited, TCR-associated ZAP-70. Although we have not ruled out an indirect association between these two molecules, we suggest that this would occur through proline-rich-SH3 domain interactions. An effector function of p120-GAP other than acting as GTPase is still unknown (45).
The demonstration of the flexibility of TCR-mediated recognition and consequent T cell responses raises an interesting question related to downstream signaling by variant TCR ligands. That is, does differential early TCR-mediated signaling result in a similar decrease in activation of all downstream pathways? Or conversely, does this differential signaling correlate with preserved activation of some pathways but not others? Our data would support a model of dissociated activation of some signaling pathways, but not others. This could reflect different requirements of sustained signaling for each signaling pathway emanating from the TCR. Such a model is also supported by two recent reports indicating that partial agonists of the TCR can induce low and transient calcium signals that do not correlate with detectable generation of inositol trisphosphates (17, 18). The different thresholds of activation for downstream pathways would then correlate with different patterns of effector T cell responses as recently reported (82). The ability to dissociate signaling pathways may have fundamental biological implications in the design of immunomodulatory strategies that target specific signaling pathways whose activation results in split T cell responses. In this sense, selective activation of the Ras-MAPK pathway may reproduce some of the effects seen after TCR engagement with partial agonist ligands.
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Footnotes |
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Address correspondence to J. Madrenas, John P. Robarts Research Institute, Rm. 2-05, PO box 5015, 100 Perth Dr., London, ON, Canada N6A 5K8. Phone: 519-663-5777, Ext. 4242; Fax: 519-663-3789; E-mail: madrenas{at}rri.on.ca
Received for publication 5 January 1998 and in revised form 3 March 1998.
We thank Dr. Ron L. Wange and the members of the Madrenas lab for helpful suggestions and comments.
This work is supported by grants from the Medical Research Council of Canada, the Kidney Foundation of Canada, the Leukemia Research Fund of Canada, and the London Health Sciences Centre. J. Madrenas is a Scholar from the Kidney Foundation of Canada.
Abbreviations used in this paper
ERK, extracellular regulated kinase;
MAPK, mitogen-activated protein kinase;
p120-GAP, p120-GTPase-
activating protein;
PCC(81-104), pigeon cytochrome c fragment 81-104;
ZAP, -associated protein.
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References |
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