By
From the * Servicio de Inmunología, Hospital de la Princesa, Universidad Autónoma de Madrid,
E-28006, Madrid, Spain; and Department of Immunology and Oncology, Centro Nacional de
Biotecnología, Consejo Superior de Investigaciones Cientificas, Universidad Autónoma de Madrid,
Campus de Cantoblanco, E-28049 Madrid, Spain
Leukocyte migration in response to cell attractant gradients or chemotaxis is a key phenomenon both in cell movement and in the inflammatory response. Chemokines are quite likely to
be the key molecules directing migration of leukocytes that involve cell polarization with generation of specialized cell compartments. The precise mechanism of leukocyte chemoattraction
is not known, however. In this study, we demonstrate that the CC chemokine receptors CCR2
and CCR5, but not cytokine receptors such as interleukin (IL)-2R, IL-2R
, tumor necrosis
factor receptor 1, or transforming growth factor
R, are redistributed to a pole in T cells that are migrating in response to chemokines. Immunofluorescence and confocal microscopy studies show that the chemokine receptors concentrate at the leading edge of the cell on the flattened cell-substratum contact area, induced specifically by the signals that trigger cell polarization. The redistribution of chemokine receptors is blocked by pertussis toxin and is dependent
on cell adhesion through integrin receptors, which mediate cell migration. Chemokine receptor expression on the leading edge of migrating polarized lymphocytes appears to act as a sensor
mechanism for the directed migration of leukocytes through a chemoattractant gradient.
Chemokines exert their effects by interacting with seven
transmembrane glycoprotein receptors coupled to a G
protein signaling pathway (1, 2). Several chemokine receptors have now been cloned and classified into two groups, CC
chemokine receptor (CCR) and CXC chemokine receptor
(CXCR) (1). Some of these receptors, including CCR5,
CCR3, and CXCR4 or fusin, have recently been described to function as coreceptors for HIV infection (see review in
reference 1). Chemokine receptor expression on T cells
seems to be tightly regulated at the transcriptional level. The
upregulated mRNA expression of several chemokine receptors
on activated and memory T cells has been described, a
phenomenon that appears to be dependent on exogenous
cytokines such as IL-2 (2, 3). Consequently, memory T cells
represent the lymphocyte subset showing a higher transendothelial chemotactic potential (4).
Cell locomotion is extremely important, especially in the
case of leukocyte migration. Certain aspects of the chemotaxis mechanism have been studied in various cell types and
experimental systems. It is now becoming clear that cell
motility involves cell polarization, with the formation of
lamellipodia at the leading edge (5), where several unrelated reports suggest that an exocytosis process occurs (6).
This promotes the redistribution of specific molecules during cell motion, such as The chemotactic response is fundamental in leukocyte
physiology and implies recognition of an external stimulus
gradient. Several mechanisms responsible for the chemotactic
response have been suggested, including those dependent on
receptor occupancy. Chemotactic receptor enrichment at the
anterior cell pole has been difficult to demonstrate, due to the
lack of specific antibodies. Preliminary evidence was shown
in early studies describing the asymmetric binding of labeled
N-formylmethionyl-leucylphenylalanine on neutrophils (10).
These studies suggested that phenomena taking place at the
leading edge might direct cell motility, but a more definitive demonstration was necessary. In this paper, we demonstrate that CCR2 and CCR5 receptors polarize to the
leading edge of migrating lymphocytes, a finding that helps
to understand the chemotactic response mechanism.
Cytokines and Reagents.
The anti-CCR2 mAb (monocyte
chemotactic protein [MCP]-1R03) was obtained and characterized as described elsewhere. Mouse anti-CCR5 antiserum was obtained by immunization with a KLH-coupled synthetic peptide
corresponding to amino acids 6-20 of the human CCR5 chemokine receptor. Recombinant human (rh) regulated on activation, normal T cell expressed and secreted (RANTES) (R&D Sys. Inc.,
Minneapolis, MN), and rhIL-8, and rhMCP-1 (PeproTech EC,
Ltd., London, U.K.) were purchased. Recombinant IL-15 derived from a simian kidney epithelial cell line was provided by
Immunex Corp. (Seattle, WA). IL-2 was provided by Hoffman-LaRoche (Nutley, NJ). PHA, poly-L-lysine (poly-L-Lys) and
PMA were purchased from Sigma Chemical Co. (St. Louis,
MO). The anti-intercellular adhesion molecule (ICAM)-3 mAb
HP2/19 (11), the anti-CD25 mAb TP1/6 (12), and the soluble recombinant proteins recombinant soluble (rs)ICAM-1 and rs
vascular cell adhesion molecule (VCAM)-1 have been previously
described (11). The tryptic 38- and 80-kD fibronectin fragments
(FN-40 and FN-80) were a gift of Dr. A. García-Pardo (Centro
de Investigaciones Biológicas, Madrid, Spain).
Cells and Cell Lines.
PBL and human T lymphoblasts were
prepared from PBMC and cultured as described (11). T lymphoblasts cultured for 7-12 d were used in all experiments.
Flow Cytometry Analysis.
CCR2 and CCR5 cDNA transiently
transfected 293 cells or human lymphoblasts were incubated with
biotinylated anti-CCR2 MCP-1R03 mAb or mouse anti-CCR5
antiserum for 30 min at 4°C. Cells were then washed twice with
PBS and labeled with phycoerythrin-labeled streptavidin or goat
anti-mouse Ig (Southern Biotechnology Assoc., Birmingham,
AL) for 20 min at 4°C. Cell-bound fluorescence was determined
in a flow cytometer (Profile XL; Coulter Corp., Hialeah, FL).
Immunofluorescence Digital Confocal Microscopy and Time-lapse
Videomicroscopy.
Immunofluorescence experiments were performed essentially as described (11). In brief, 2 × 106 T lymphoblasts in 500 µl complete medium were allowed to adhere to
protein-coated coverslips in 24-well plates (Costar Corp., Cambridge, MA). Cytokines and chemokines (10 ng/ml) were added, and cells were incubated at 37°C in a 5% CO2 atmosphere. After 30 min, cells were fixed with 3.7% formaldehyde in PBS for 10 min
at room temperature and stained with specific mAb plus an FITC-labeled rabbit F(ab We have generated an mAb to the CCR2
receptor, a receptor that specifically binds MCP-1, -3, and
-4 (14), and a mouse polyclonal antiserum to CCR5, a receptor for RANTES, macrophage inflammatory protein 1
Cell migration directed by chemical attractant gradients
requires cell polarization, but the fine mechanism by which
this directional response is achieved remains to be described
(8, 9, 16, 17). We studied the cellular distribution of chemokine receptors during T lymphocyte migration. CCR2 and
CCR5 were evenly distributed throughout the T lymphoblast cell membrane (Fig. 2, A and C). Upon incubation of
T lymphoblasts with MCP-1 or RANTES, a rapid cell polarization takes place that results in the redistribution of
CCR2 and CCR5 receptors to the leading edge of the cell
(Fig. 2, B and D). In the presence of a chemokine gradient,
the cluster of CCR2 was clearly oriented towards the chemoattractant source. Parallel videomicroscopy studies showed
that polarized cells correspond to migrating cells (Fig. 3).
IL-2R
During migration, lymphocytes are polarized such that
the leading edge of the cell develops cytoplasmic extensions
(lamellipodia), whereas the posterior part of the cell forms
an appendage (the uropod) (16, 17). Two-color fluorescence microscopy analyses allow us to conclude that the
CCR2 and the CCR5 receptors are located at the advance
front of migrating lymphocytes (Fig. 4, and data not shown).
This is confirmed by confocal microscopy studies showing
its presence on the flattened cell-substratum contact area at
the leading edge. On the opposite pole of the cell, ICAM-3
was found to decorate the uropod that protrudes from the
contact area of migrating T lymphocytes with endothelial
or extracellular matrix substrates (Fig. 4, a and b; references
11, 12).
The redistribution
of either CCR2 or CCR5 to the leading edge of the cell
was triggered by the chemokines MCP-1, RANTES, and IL-8, as well as by the cytokines IL-2 and IL-15 (Fig. 5, a
and b), known to be chemotactic factors (12, 18, 19). In contrast, the proinflammatory cytokine TNF-
It has been established that chemokines regulate integrin-mediated cell adhesion to extracellular matrix and endothelium (21). Here we show that CCR2 polarization is
induced by MCP-1 and RANTES when T lymphoblasts
adhere to the integrin ligands FN-80 and FN-40, to the
endothelial counter-receptor ICAM-1, and to VCAM-1,
but not on T cells immobilized on poly-L-Lys (Fig. 5 c).
Leukocyte adhesion through integrins is required to induce
cell polarization (11) and redistribution of chemokine receptors, but not of the cytokine receptors tested. These
data further support the model that chemokines may act in
combination with adhesion molecules to steer leukocyte
traffic to tissues (22).
Leukocyte motion involves several phenomena, including changes in cell shape, integrin affinity, and integrin recycling at the cell's leading edge (7, 8). These events appear
to be mediated by phosphorylation signals through chemokine receptors, although the signal transduction machinery
implicated has not been fully elucidated (23). Chemokine
receptors are coupled to a G protein signaling pathway, and
we previously reported that chemokine-induced lymphocyte polarization is abrogated by pertussis toxin (11). We
have now found that pertussis toxin treatment of T lymphocytes also abolishes chemokine-mediated CCR2 polarization (Fig 5 d), indicating a requirement for a signal triggered by the chemokine receptor.
Leukocyte migration is a rather complex phenomenon,
where chemokines are very important players. The patching
of chemokine receptors to the leading edge of the cell implies two remarkable consequences: first, the functional specialization of this cell domain in signal transduction, and
second, the establishment of an endogenous polarity in the
cell, which may be crucial for chemotaxis and other immune responses involving chemokines. Finally, it is worth insisting that the clustering of chemokine receptors to the advance front of migrating cells is induced by the polarization process itself, regardless of the polarizing or chemotactic
agent involved. Hence, the redistribution of CCR2, CCR5
(described above), and CXCR3 (data not shown) to the
leading edge of cells adopting the polarized migrating phenotype, may also apply to other chemokine receptors. In
fact, although not yet characterized, an early report showed
the asymmetric distribution of the labeled N-formyl peptide bound to polarized neutrophils, mainly in the mid-region of the cell and at a lower density on the tail (10).
Finally, lymphocyte polarization is involved in many processes such as antigen presentation, differentiation, activation, target cell recognition by cytotoxic T lymphocytes, and cell
killing (24). Polarization of the chemokine receptors
may act as a sensor mechanism to increase cell responsiveness to chemokines and to guide cell-cell interaction, as
well as to marshal lymphocyte motion along a chemokine
gradient during lymphoid cell trafficking in immune and
inflammatory responses.
v
3 (7),
actin (8), or the urokinase type plasminogen receptor (9).
)2 anti-mouse IgG (Pierce Chem. Co., Rockford, IL). For directional migration assays, experiments were performed as above, but 1 µl of chemokine (1 µg/ml) was deposited at the lower edge of the well, and T lymphoblasts were allowed to migrate. Kinetic studies showed that chemokine gradient effects were observed from 30 min up to 2 h after chemokine application. For double-label studies, cells were incubated with HP2/19
mAb cells followed by rhodamine-labeled rabbit F(ab
)2 anti-mouse IgG (Pierce Chem. Co.). Samples were washed, saturated with
10% mouse serum, and incubated with a 1:50 dilution of the biotinylated MCP-1R03 mAb, followed by washing and labeling
with FITC-avidin D (Vector Labs., Inc., Burlingame, CA). Using
a photomicroscope with ×60 oil immersion objectives (Labophot-2; Nikon Inc., Melville, NY), the proportion of CCR polarization was calculated by random choice of 10 fields for each
condition and direct cell counting (No. 400-500). Preparations
were photographed on Ektachrome 400 film. For confocal studies, cells adhering to FN-80-coated (20 µg/ml) coverslips and
stimulated with MCP-1 were stained with MCP-1R03, followed
by incubation with Cy2 goat anti-mouse IgG (Amersham, Pittsburgh, PA), saturation with 10% mouse serum, and staining with
biotinylated anti-ICAM-3 HP2/19 and Cy3 streptavidin (Biological Detection Systems Inc., Pittsburgh, PA). Confocal microscopy
was performed (MRC-1024 Confocal Laser Scanning System; Bio
Rad Labs., Richmond, CA). Videomicroscopy analysis was performed as described (13).
Chemokine Receptor Polarization in Activated Human T
Lymphocytes.
,
and macrophage inflammatory protein 1
(15). The anti-CCR2 mAb MCP-1R03 reacts with the 293 cell line
transiently transfected with CCR2 cDNA (Fig. 1 A), but not
with CCR5 cDNA-transfected cells (Fig. 1 B). Conversely,
the CCR5-specific antiserum recognizes only CCR5, but not
CCR2 cDNA-transfected cells (Fig. 1 B). Membrane expression of CCR2 and CCR5 was virtually undetectable on
freshly isolated peripheral blood T lymphocytes (Fig. 1 C,
and data not shown), whereas a significant fraction of IL-2-
cultured, PHA-activated T lymphoblasts (>50%) express both
receptors (Fig. 1, D and E).
Fig. 1.
Recognition of human CCR2 and CCR5 chemokine receptors in transfected 293 cells and PBL. Flow cytometry analysis of the
binding of the anti-CCR2 mAb MCP-1R03 (A) and mouse anti-CCR5
antibody (B) to the 293 cell line, transiently transfected with human CCR2 (white histogram) and CCR5 (shaded histogram) cDNA. CCR2 expression was assessed using the anti-CCR2 MCP-1R03 mAb in resting (C) or PHA-activated IL-2-cultured human PBL (D). Expression of the CCR5 in activated human PBL is shown (E). Shaded histograms correspond to
the isotype-matched mAb (C and D) and irrelevant mouse antiserum (E)
negative-staining controls. Cells were incubated with biotinylated anti-CCR2 mAb MCP-1R03 or mouse anti-CCR5 antiserum for 30 min at
4°C. Cell-bound fluorescence was determined in a flow cytometer (Profile XL; Coulter Corp.).
[View Larger Version of this Image (30K GIF file)]
, IL-2R
, TNFRI, and TGF-
type II receptor expression was also analyzed and found to undergo neither
redistribution nor clustering during polarization (Fig. 2, A
and B, insets, and data not shown).
Fig. 2.
The CCR2 and the
CCR5 receptors redistribute to
the leading edge of the polarized
migrating T cells. PHA-activated
T lymphocytes, untreated (A and
C), or treated with 10 ng/ml
MCP-1 (B) or 10 ng/ml
RANTES (D), were allowed to
adhere to coverslips coated with
20 µg/ml FN-80 (A and B) or
10 µg/ml rsICAM-1-Fc (C and
D), and then fixed and stained
with the anti-CCR2 mAb
MCP-1R03 (A and B) or the
anti-CCR5 mouse antiserum (C
and D), as described in Materials
and Methods. Cells were photographed under epifluorescent conditions. Original magnification: ×1,200. Bar, 10 µm. Insets show staining of the IL-2R
with the TP1/6 mAb on a resting (A) and a polarized (B) T
lymphoblast. Original magnification: ×600.
[View Larger Version of this Image (15K GIF file)]
Fig. 3.
Migrating T cells
polarize the CCR2 receptor towards the chemoattractant gradient. (A-C) Time-lapse videomicroscopy analysis of migrating T
lymphoblasts in response to
MCP-1. Cells were allowed to
adhere to 10 µg/ml rsICAM-1
on coverslips; MCP-1 was then
deposited at one edge of the bottom of the well and cells allowed
to migrate. Sequential time
frames are shown. Polarized migrating lymphocytes show the phase-dark leading edge and the phase-bright uropod. (D) Parallel immunofluorescence studies show the CCR2 distribution of the migrating cells. Cells were photographed with a ×60 objective. Bar, 10 µm. The arrows indicate the direction of cell migration towards the
chemokine source.
[View Larger Version of this Image (44K GIF file)]
Fig. 4.
(a) The CCR2 receptor
patches on the cell-sustratum contact
areas at the leading edge of the migrating T cell. Confocal microscopy was performed as described in Materials and Methods. Three representative optical cell sections are shown,
out of the eight obtained. Serial sections are from the upper level (left,
2.464 µm), which shows staining of
ICAM-3 (red fluorescence) at the uropod, to the sustratum level (right,
3.92 µm), where CCR-2 (green fluorescence) is found at the leading edge. (b) Polarized distribution of the CCR2 receptor and ICAM-3 on migrating T cells. Cells adhered to FN-80-coated coverslips were double stained with anti-ICAM-3 HP2/19 (yellow fluorescence) and anti-CCR2 mAb MCP-1R03 (green fluorescence). Original magnification:
×1,200. Bar, 10 µm. The inset shows another T lymphoblast from the same sample at equal magnification.
[View Larger Versions of these Images (6 + 7K GIF file)]
, which does not
induce cell polarization, exerted no effect. PMA, which promotes cell spreading and blocks cell migration, decreased
the basal level of CCR polarization (Fig. 5, a and b). Other
T cell polarization-inducing agents, such as mAb against
ICAM-3 and CD43 (11, 20), also triggered CCR2 redistribution (data not shown). These data indicate that CCR
polarization in leukocytes correlates with the acquisition of
the migrating phenotype and is independent of the chemoattractant stimulus. Anti-MCP-1 or CCR2 mAb that block
chemotaxis also inhibited the acquisition of the polarized
morphology and the redistribution of CCR2 to the leading
edge (data not shown). The sum of these results reveals the
domain specialization that takes place in activated, migrating T lymphocytes. The receptor involved in detection of
the chemoattractant gradients is located at one pole, whereas at the other pole, the receptor implicated in cell adhesion
recruits bystander leukocytes (13).
Fig. 5.
(a and b) The role of
chemoattractants in the redistribution of CCR2 and CCR5 receptors on migrating T lymphocytes. T lymphoblasts stimulated
with 10 ng/ml of several polarizing chemokines and cytokines
were allowed to adhere to coverslips coated with 20 µg/ml FN-80 (a) or 10 µg/ml rsICAM-1-Fc
(b). Cells were then stained and
the percentage of cells on which
the CCR2 (a) or CCR5 (b) receptors were redistributed was
calculated as described. The arithmetic mean ± SEM of four (a)
and three (b) independent experiments is shown. (c) The role of
various substrates on CCR2 receptor polarization. T lymphoblasts were allowed to adhere to
coverslips coated with 10 µg/ml
rsICAM-1-Fc, 20 µg/ml FN-80, 30 µg/ml FN-40, 20 µg/ml
rsVCAM-1-Fc, or 20 µg/ml
poly-L-Lys, and then stimulated with chemokines or PMA (10 ng/ml). Cell samples were processed as described. The arithmetic mean ± SEM of three independent experiments is represented. (d) Chemokine-induced polarization of the CCR2 receptor is blocked by pertussis
toxin. PHA-activated T lymphocytes were pretreated with pertussis toxin (1 µg/ml) for 20 min at 37°C, and then allowed to adhere to rsICAM-1 (as in
the legend to Fig. 2). The effect of the chemokines on CCR2 redistribution to the leading edge of cells was quantified as in Fig. 2. The arithmetic mean ± SEM of three independent experiments is shown.
[View Larger Version of this Image (28K GIF file)]
Address correspondence to Francisco Sánchez-Madrid Servicio de Inmunología, Hospital de la Princesa, Universidad Autónoma de Madrid, Diego de León 62, E-28006, Madrid, Spain. Phone: 34-1-402-3347; FAX: 34-1-309-2496; E-mail: fsmadrid/princesa{at}hup.es
Received for publication 15 January 1997 and in revised form 18 April 1997.
We thank Drs. A. Aragay and J. Gutierrez for the CCR2B and CCR5 transfectants, respectively, M.A. Ollacarizqueta and E. de la Rosa for technical assistance with confocal microscopy, C. Cabañas for assistance with the time-lapse videomicroscopy studies, M.A. del Pozo for excellent advice on the experimental work, both him and Dr. R. Gonzalez-Amaro for critical reading of the manuscript, and C. Mark for editorial assistance.
This work was supported by grant SAF 96/0039 from the Ministerio de Educación y Ciencia, grant 07/44/ 96 from Comunidad Autónoma de Madrid, and a grant from Asociación de la Lucha contra el Cancer (to F. Sánchez-Madrid). The Department of Immunology and Oncology was founded and is supported by the Consejo Superior de Investigaciones Cientificas and Pharmacia & Upjohn.
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