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Address correspondence to Arne N. Akbar, Dept. of Immunology and Molecular Pathology, Div. of Infection and Immunity, University College London, 46 Cleveland St., London W1T 4JF, England, UK. Phone: 44-207-679-9214; Fax: 44-207-679-9119; email: a.akbar{at}ucl.ac.uk
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Abstract |
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Key Words: senescence inflammation differentiation proliferation cytokines
Abbreviations used in this paper: BCG, Bacille Calmette-Guerin; flow-FISH, flow cytometric detection of fluorescence in situ hybridization; HDA, heteroduplex analysis; MT, Mantoux test; PPD, purified protein derivative; SB, suction blister.
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Introduction |
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It has been shown that the loss of replicative capacity due to telomere erosion can also regulate memory T cell maintenance (8). Telomeres are repeating hexameric sequences of DNA that are located at the ends of chromosomes (9). Each cell division leads to the loss of 50100 bp of telomeric DNA due to the inability of DNA polymerase to fully replicate the ends of the chromosomes (10, 11). Although the induction of the enzyme telomerase during a primary response to antigen can compensate for telomere loss in antigen-specific T cells in vivo (1214), upon repeated stimulation, T cells lose the ability to induce this enzyme, resulting in telomere erosion (15). This leads eventually to cell cycle arrest and replicative senescence; this is a mechanism that determines the residual replicative capacity of proliferating T cells (1618).
The lack of suitable models has restricted investigations into mechanisms that regulate human memory T cells in vivo. This work was designed to establish an experimental system for the investigation of the kinetics of human memory T cell proliferation, differentiation, and telomere erosion during a secondary response to characterize factors that may regulate the persistence of these cells in situ. To do this, we injected purified protein derivative (PPD) into the skin of individuals who were immunized with Bacille Calmette-Guerin (BCG) to induce a secondary CD4+ T cell response, also known as the Mantoux test (MT). Responding T cells were isolated from skin suction blisters (SBs) that were induced over these lesions at different times (19). We found that there was considerable proliferation of PPD-specific CD4+ T cells during the secondary response in the skin and demonstrate that these cells differentiated to a CD45RBlo phenotype. Furthermore, we demonstrate for the first time that telomere erosion occurs in specific memory CD4+ T cells during a secondary response in a nonlymphoid site and that this results in part from inhibition of telomerase in these cells by type I IFN in situ.
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Materials and Methods |
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Skin SBs.
Skin SBs were induced by the application of a negative pressure of 2540 kPa (200300 mmHg) below atmospheric pressure via a suction chamber for 24 h using a clinical suction pump (VP25; Eschmann) until a unilocular blister measuring 1015 mm in diameter was formed. SBs were raised over the sites of PPD injection (MTs) or normal skin 1824 h before sampling to ensure maximum cell recovery. The blister fluid was microcentrifuged at 650 g for 4 min to pellet the cells present. The pellet was resuspended in complete medium (RPMI 1640; Invitrogen and Life Technologies) containing 10% human AB serum, 100 U/ml penicillin, 100 µg/ml streptomycin, and 2 mM L-glutamine (all obtained from Sigma-Aldrich). Blister CD4+ T cells were purified by negative selection. Blister cells were first incubated with antibodies against CD8, CD14, CD16 (Beckman Coulter), CD19, and glycophorin A (Beckman Coulter), and these cells were added to plates coated with rabbit antimouse immunoglobulins (DakoCytomation).
PBMC Preparation.
Heparinized blood was collected from the same individuals at the time of blister aspiration. PBMCs were prepared by density centrifugation on Ficoll-Paque (Amersham Biosciences). CD4+ T cells were isolated by positive or negative selection using the VARIO MACS (Miltenyi Biotec). CD45RO+ populations were isolated by positive selection.
Flow Cytometric Analysis.
Four-parameter analysis of T cell phenotype was performed on a FACSCaliburTM (Becton Dickinson) as described previously (21). Cells were enumerated after staining with fluorochrome-conjugated CD3, CD4, CD8, and/or Ki67 using TruCOUNTTM tubes (all obtained from Becton Dickinson). Other reagents were used as follows: CD45RA-FITC, IFN-APC, IFN-
FITC, IL-2FITC, and Ki67-FITC (all obtained from Becton Dickinson); and CD4-PE, CD45RA-PE, and CD45RB-FITC (all obtained from DakoCytomation).
Intracellular Cytokine Staining.
SBs or PBMCs were stimulated with 10 µg/ml PPD (Statens Serum Institut) or 1:1,000 dilution tetanus toxoid (Aventis Pasteur MSD Ltd.) and incubated for 15 h at 37°C in a humidified 5% CO2 atmosphere. 5 µg/ml brefeldin A (Sigma-Aldrich) was added after 2 h. Unstimulated controls were also included. The cells were fixed and permeabilized (Fix & Perm® Cell Permeabilisation Kit; Caltag Laboratories) before staining for CD3, CD4, IL-2, and IFN-.
Measurement of Telomere Length by Flow Cytometric Detection of Fluorescence In Situ Hybridization (Flow-FISH).
Telomere length of CD4+ T cells was measured using a modified two-color flow-FISH protocol (21). The cells were stained with CD4-biotin (Immunotech) followed by streptavidin-Cy3 (Cedarlane Laboratories Ltd.), after which samples were fixed and permeabilized (Fix & Perm® Cell Permeabilisation Kit; Caltag Laboratories). After washing in hybridization buffer, cells were incubated with 0.75 µg/ml of the PNA telomeric (C3TA2)3 probe conjugated to Cy5. Samples were heated for 10 min at 82°C, rapidly cooled on ice, and hybridized for 1 h at room temperature in the dark. Samples were washed and analyzed immediately by flow cytometry. Fluorescently labeled beads (DakoCytomation) were used to standardize the cytometer settings. No probe controls were included to allow for differences in background fluorescence between samples. In addition, two cryopreserved PBMC samples with known telomere fluorescence were used as standards to ensure consistency of the results.
To measure telomere length of Ag-specific CD4+ cells, we developed a three-color flow-FISH technique. SBs or PBMCs were stimulated with PPD for 15 h as aforementioned. After surface staining with CD4-biotin and streptavidin-Cy3, samples were fixed, permeabilized, and stained with IFN-FITC before hybridization with the telomeric probe.
Telomerase Activity.
Telomerase activity was measured using the telomeric repeat amplification protocol (TRAPeze Telomerase Detection Kit; Intergen Company). In brief, telomerase present in a test cell extract extends a template with telomeric repeats and, after PCR amplification, generates a ladder of products with 6-bp increments starting at 50 nucleotides. Samples were collected by the snap freezing of cells either recovered from SBs or from in vitro cultures at various time points after PPD injection or stimulation, respectively. Absolute numbers of CD3+Ki67+ cells in each sample were enumerated using Tru-count tubes and Ki67 analysis. PCR was performed with samples adjusted to 500 Ki67+ T cells per reaction. The negative control contains the PCR mix without cell extract, and the positive control contains an extract of a telomerase positive tumor cell line.
Type I IFN Inhibition Experiments.
To investigate the effect of blister fluid on telomerase up-regulation in vitro, fresh day 3 blister fluid was added to 0.5 µg/ml PPD-stimulated PBMCs from the same donor (50% final dilution). In addition, autologous serum was also added to PPD-stimulated PBMCs as a control (25% final dilution). To block the effects of type I IFN, anti-CD118 antibody (antiIFN-, receptor chain 2; Calbiochem) or isotype control antibody (mouse IgG2a; Sigma-Aldrich) was added at 2.5 µg/ml to samples cultured in the presence of MT blister fluid. Samples for telomerase measurement were collected on day 3 after PPD stimulation, and proliferation was measured on day 5. To investigate the direct inhibition of telomerase activity by type I IFN in vitro, PPD-specific CD4 T cell lines generated from normal donors were restimulated in the presence of various doses of recombinant IFN-
(subtype 2a; R&D systems) or Roferon, a clinically used preparation of IFN-
(subtype 2a; Roche Pharmaceuticals). After 4 d, the cells were assayed for telomerase activity. Samples were adjusted to 500 Ki67+ T cells per reaction as aforementioned.
Long-Term Cell Culture.
Cells were isolated from blisters induced 19 d after PPD challenge in vivo and restimulated with PPD-pulsed, irradiated autologous PBMCs in vitro. Cell lines were maintained in complete medium supplemented with 5 ng/ml IL-2 and restimulated with 1 µg/ml PPD-pulsed autologous irradiated PBMCs every 1014 d. Fresh medium and IL-2 were added every 34 d. Cell numbers and viability were quantified with Tru-count tubes. Population doublings (PD) were calculated using the following equation: PD = log (number of cells counted after expansion) log (number of cells seeded)/log2.
Histological Analysis of Skin Biopsies.
Frozen 6-µm sections of skin biopsies were fixed and immunostained using indirect immunoperoxidase, double indirect immunofluorescence, indirect alkaline phosphatase, and TUNEL methods as described previously (20). The number of positive cells was quantified by image analysis using a circular 100-µm diameter circular frame centered on the five largest perivascular infiltrates per section as described previously (20). Unconjugated antiIFN- antibody (C10F5; murine IgG1) was purchased from Serotec Ltd.
Measurement of Type I IFN in Blister Fluids.
The amount of type I IFN in blister fluid was assayed by a standard antiviral assay (22). The concentration of type I IFN was determined by comparison with a standard curve prepared from IFN- at known concentrations (IFN-
N1, Wellferon; GlaxoSmithKline).
Assessment of Clonality by Heteroduplex Analysis (HDA).
The clonal composition of purified CD4+ T cells isolated from blisters at days 7 or 19 after PPD challenge in vivo or CD4+ T cells from blood from the same volunteers was determined by comparing the CDR3 region of different TCR Vß families. Blood CD4+ T cells were collected at day 0 and cultured either with or without PPD and autologous irradiated PBMCs for 7 d in vitro. PCR was performed on 26 Vß families (Vß 124 including Vß 5.1 and 5.2 and Vß 13.1 and 13.2) using specific Vß and common Cß primers at each time point as described previously (23). Each sample PCR was mixed with Vß-matched carrier DNA, denatured, and reannealed. The product was run on a 12% polyacrylamide gel, which was blotted and hybridized with a probe to the external Cß region of the carrier. The probe was detected using antidigoxigenin-AP Fab fragments and CDP-Star substrate (Roche Diagnostics). Identical clones within PBMCs and CD4+ T cell populations were detected as heteroduplex bands with identical migration patterns (23). The presence of heteroduplices has been shown previously to relate to the presence of an expanded clone as determined by the sequencing of PCR product (23).
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Results |
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Lymphocytes constituted the majority of the leukocytes in SBs (Fig. 1 c), and >98% of these were CD3+ T cells (not depicted). Other leukocytes found in the blister populations included CD68+ monocytes and macrophages as well as smaller numbers of CD1a+ Langerhans cells and DCs (unpublished data). The proportion of CD4+ T cells increased significantly from 57.7 to 88% on days 3 and 19, respectively, after antigenic challenge (Kruskal-Wallis test; P = 0.0006), whereas expression in the peripheral blood did not change significantly during this period (Fig. 1 d). Greater than 95% of the CD4+ T cells that were harvested from skin blisters or identified within biopsies were of the CD45RA (CD45RO+) primed/memory phenotype as compared with 50% of CD4+ T cells in the blood (see Fig. 4 a), supporting previous observations that the MT is a CD4+ memory, T cellmediated immune response (5, 20).
Specific CD4+T Cells Proliferate at the Site of Antigenic Challenge.
We evaluated the proportion of antigen-specific CD4+ T cells that were isolated from the skin during the MT by their ability to synthesize IFN- after restimulation with PPD in vitro (Fig. 2 a). Minimal IFN-
was synthesized when PPD was not added to the blister cells or when the cells were stimulated with tetanus toxoid (Fig. 2, a and b). Therefore, the cells that expressed IFN-
were Ag-specific (PPD). We found a significant increase in these cells after intradermal challenge with PPD (Fig. 2 b; Kruskal-Wallis test; P < 0.0001). There was no increase in Ag-specific CD4+ T cells in the blood, and these remained relatively constant at 0.5% of the CD4+ T cell pool throughout the course of the response (Fig. 2 b). The maximum proportion of Ag-specific CD4+ T cells detected after antigen challenge (32% of total CD4+ T cells) was considerably higher than that predicted from previous studies (0.52%; reference 24). The proportion of CD8+ Ag-specific T cells in the blisters did not increase during the course of the response and was <1% at all time points.
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We are confident that the SB protocol actually samples cells that are responding in situ and not just those that are migrating away for the following reasons. First, the phenotype of CD4+ T cells in tissue sections and SBs was identical (CD45RO+CD45RA). Second, the proportions of CD4+ T cells that were proliferating and the kinetics of proliferation were virtually identical when these cells were investigated by histology or in the blister samples, indicating that we were investigating the same responding population (Fig. 2 d). Third, the yield of cells obtained from blisters over the course of the aforementioned Mantoux reaction parallels the numbers of leukocytes that were observed by histology at different times after PPD injection (20).
Our results highlight that there is significant T cell activity long after the perceived clinical course of the skin test reaction. This is because previous studies have not generally sampled the skin beyond 4 d after antigen injection (19, 26). The continued T cell reactivity in the skin of our system between days 7 and 19 probably reflects both the continued presence of residual antigen and the expansion of these cells by cytokines that are present in situ (5, 20).
The Same CD4+ T Cell Clones Persist during the Course of the MT.
It was unclear how the turnover of CD4+ T cells at the site of PPD injection affected the clonal composition of CD4+ T cells during the course of the response. To assess potential changes in the clonal distribution, we used HDA (27). Unstimulated CD4+ T cells from blood showed a polyclonal smear after HDA (Fig. 3 a). However, upon stimulation of these cells in vitro with PPD, clonal bands were detected (Fig. 3 a). CD4+ T cells isolated from blisters on day 7 after PPD challenge showed a highly oligoclonal distribution (Fig. 3 a). Virtually all the clones identified in the blister samples at day 7 were also found in PPD-stimulated PBMCs from the same individuals (9396%), indicating that the clonal bands in blister samples represented PPD-specific clones (Fig. 3 a). Of the clones identified in stimulated blood cells, only 66% (range 4485%) were present in the skin at day 7, suggesting that not all CD4+ T clones that were found in blood could enter or survive in the skin.
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CD4+ T Cells Differentiate in the Skin during a Secondary Response.
We investigated CD45RB expression as an indication of the extent of CD4+ T cell differentiation in situ. This molecule is highly expressed in CD45RA+CD4+ naive T cells, but shows bimodal distribution on primed/memory CD45RARO+ CD4+ T cells (28). Memory CD4+ T cells that are CD45RBlow are more differentiated and have shorter telomeres compared with those that are CD45RBhigh cells (unpublished data).
During the course of the MT, virtually all the CD4+ T cells isolated from the skin were CD45RACD45RO+ in contrast with the CD4+ population from blood from the same individuals, where many CD45RA+ T cells were observed (Fig. 4 a). When we compared CD45RB expression on CD45RACD45RO+ (primed) CD4+ T cells from blood and blisters in the same individuals on days 3 and 7 during the induction and peak phases of the response, respectively, no difference was observed (Fig. 4 b). However, by day 19, a significantly greater number of CD4+ T cells from the skin showed low CD45RB expression compared with equivalent populations from paired blood samples. This indicated that primed Ag-specific CD4+ T cells undergo further differentiation in situ after PPD challenge (Fig. 4 b). To confirm this, we measured the telomere length of CD4+ T cells in the skin using flow-FISH (21). Because all CD4+ T cells in the skin blisters are CD45RO+, we compared their telomere lengths with blood CD4+CD45RO+ T cells. At day 7 after PPD injection, the telomere length of CD4+ T cells in the SBs was similar to that found in CD4+CD45RO+ T cells from blood samples in the same individuals (Fig. 4 c). However, by day 19, the telomere length of CD4+ T cells from the skin was significantly shorter than the equivalent population in blood, confirming that they were more differentiated. The decrease in telomere mean fluorescence intensity in blister samples observed at day 19 equates to the loss of 0.4 kb of telomeric DNA as determined using calibration curves correlating telomere length (in kilobases) measured by Southern blot analysis with the flow-FISH technique as described previously (21).
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Telomerase Activity in the Skin.
We investigated whether telomere erosion in CD4+ T cells after PPD stimulation in vivo resulted from lack of telomerase induction in these cells. At days 3 and 7 after PPD challenge, there was extensive CD4+ T cell cycling as 7.7 ± 2.0% and 17.8 ± 6.2% of these cells, respectively, were Ki67+. The extent of cell cycling was greater than that observed when CD4+ T cells from blood were stimulated by PPD in vitro for 3 and 7 d, respectively (2.4 ± 0.4% and 9.7 ± 4.0% Ki67+). Because telomerase is only up-regulated in the activated proliferating population of T cells (29, 30), we standardized telomerase assays to include an equivalent number of Ki67+ T cells in each sample. For the first time, this allowed a direct comparison of the activity of this enzyme in cycling CD4+ T cells from both blood and blister at different times after stimulation (30, 31). Telomerase activity was minimal in cycling CD4+ T cells that were isolated from the skin compared with CD4+ T cells from blood that were stimulated by PPD in vitro (Fig. 5, a and b). This indicated that telomere erosion in PPD-specific CD4+ T cells was linked to the lack of telomerase activity in these cells in vivo.
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Type I IFN within the MT Inhibits Telomerase Induction.
Our previous studies indicate that type I IFN (IFN- and IFN-ß) can regulate the rate of differentiation of human T cells (32). Together with reports that IFN-
can inhibit the activity of telomerase in tumor cell lines in vitro (33), this prompted us to determine whether this group of cytokines was responsible for the inhibition of telomerase activity in PPD-specific CD4+ T cells in the skin in situ. By immunohistological analysis, we identified cells that expressed high levels of IFN-
in the perivascular infiltrates of skin sections 3 d after PPD challenge (Fig. 6 a). High uniform levels of expression were also detected in keratinocytes (Fig. 6 a). This was confirmed by measurement of peak concentrations of type I IFN in SB fluid that was obtained at day 3 after PPD injection (Fig. 6 b). This coincided with the time at which telomerase was greatest in CD4+ T cells that were PPD-stimulated in vitro, but absent from cycling CD4+ T cells in the skin (Fig. 5 b).
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Next, we examined the capacity of CD4+ T cells that were removed from the skin microenvironment to expand in response to repeated stimulation with PPD stimulation in vitro (Fig. 6 e). These cells were able to expand in culture, and this was associated with their ability to up-regulate telomerase after restimulation in vitro (Fig. 6 f). However, despite the optimal stimulation of the skin-derived CD4+ T cells with PPD in vitro, the cells reached replicative senescence at day 125 after eight episodes of restimulation, after which no further expansion of the cells occurred (Fig. 6 d). This was associated with the loss of telomerase inducibility in these cells (Fig. 6 e). The loss of telomerase activity in the repeatedly stimulated cells was not reversed by the addition of antiIFN-R2 antibodies (unpublished data), suggesting that a different mechanism of telomerase down-regulation was in operation.
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Discussion |
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The intense proliferative activity was accompanied by an increase in CD4+ T cell apoptosis during the course of the response, indicating that there was extensive T cell turnover during this secondary response in the skin. Previous studies using histological investigation of Mantoux lesions indicate that the proliferation during this turnover may be driven in part by the presence of IL-2 and IL-15 within the skin after PPD injection (5). However, the apoptosis that was observed was induced by different mechanisms at the peak and resolution phases of the response (5). Although the death of the proliferating CD4+ T cells at the height of the response was linked to activation-induced cell death associated with elevated expression of CD95L, the apoptosis during the resolution phase was linked to cytokine starvation associated with down-regulation of the antiapoptotic molecule Bcl-2 (5). Because >80% of the CD4+ T cell clones that were found 21 d after PPD injection were also present early in the response at day 7, it is likely that proliferation and death are both occurring within the same antigen-specific T cell population that is responding to PPD within the skin.
We have made several unexpected observations in this work. First, the kinetics of the clinical response to PPD injection is different from the kinetics of antigen-specific T cell infiltration. Although the former peaks at 23 d when very few antigen-specific CD4+ T cells are present, the latter peaks between 715 d, when the clinical response is markedly reduced. The events that lead to the recruitment of the specific cells require further investigation. A second new observation was that antigen-specific CD4+ T cells were differentiating within the skin after PPD injection. This was indicated by the significant proliferation and loss of CD45RB expression by the responding CD4+ T cell population. The down-regulation of CD45RB by human CD4+ T cells has been previously shown to be associated with profound changes in the capacity for cytokine secretion (28). The functional changes that accompany the differentiation of PPD-specific CD4+ T cells in the skin are currently under investigation.
Telomeres have been shown to erode as cells undergo extensive proliferation and T cells from elderly individuals have shorter telomeres than young subjects (15). However, the extent to which telomeres in memory T cells erode after a single secondary challenge with antigen is unknown. Therefore, it is of considerable interest that we observed an average loss of 400 bp of telomere DNA in the CD4+ T cells that were responding in the skin 3 wk after the injection of PPD. Because telomeres in human CD4+ T cells have been shown to decrease by an average of 50 bp/yr, this loss of telomeres was equivalent to that expected after 8 yr of normal aging in vivo (8, 18). At this rate of telomere erosion, one prediction would be that cells that continuously proliferate in the skin would reach senescence rapidly, where growth arrest occurs because telomeres are unable to fulfil their protective function (9). However, the investigation of antigen-specific T cells in draining and nondraining lymph nodes of telomerase-deficient mice (38) and animals with short telomeres (of equivalent length to that in humans; reference 12) will be required to assess directly whether telomere erosion limits uncontrolled T cell proliferation during secondary responses in vivo.
The accelerated telomere erosion during the secondary response in the skin was due in part to the active, but reversible, inhibition of telomerase in proliferating T cells by type I IFN. Immunohistological analysis showed that a constituent of the type I IFN that was detected at day 3 after PPD challenge was IFN-. However, IFN-ß has also been shown previously to be present at day 3 during this response (5). Because all the type I IFNs signal through the same receptor (39), we do not exclude the possibility that more than one member of this family of mediators contributes to telomerase inhibition. Although telomerase activity in CD4+ T cells that were stimulated with PPD in vitro was substantially inhibited by the addition of type I IFN, we could not completely inhibit the activity of this enzyme with these cytokines. Extremely low telomerase activity that was found in proliferating cells in the skin suggests that other factors may also contribute to inhibition of this enzyme in the antigen-specific cells in vivo.
Although telomerase activity in T cells is inhibited by type I IFN, it can be up-regulated in some T cells by cytokines such as IL-7 (40). These results clearly demonstrate that different cytokines may have a profound impact on the replicative lifespan of T cells. Apart from cytokine-mediated regulation of telomerase, intrinsic down-regulation of enzymatic activity occurs in T cells that are activated repeatedly and may be related to end-stage differentiation (9, 10). Telomerase inhibition by this second pathway is irreversible, but senescence in these cells can be bypassed by telomerase transfection (41, 42). The signaling pathways that lead to telomerase down-regulation after repeated stimulation or after addition of type I IFN are poorly defined but may involve inhibition of CD28 expression (16), transcriptional regulation, capacity for enzyme translocation from cytoplasm to the nucleus, and also chromatin remodelling of the telomerase gene (15). These pathways require further investigation as they may provide targets for manipulating the retention or loss of specific T cells in vivo.
In summary, we demonstrate that antigen-specific CD4+ T cells can differentiate extensively outside lymphoid tissue during a secondary response in humans in vivo. This indicates that it is essential to investigate immune responses within the context of the tissue where they are taking place. Although this poses significant constraints on the study of human immunity, the use of appropriate animal models, such as telomerase (38) or type I IFNR-deficient animals (4), will enable the clarification of specific questions that relate to human T cell differentiation during secondary responses in vivo.
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Acknowledgments |
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This work was funded by grants from the Biotechnology and Biological Sciences Research Council Experimental Research on Aging Initiative, Research Into Aging, The Sir Jules Thorne Research Trust, and The Hayward Foundation and Dermatrust.
Submitted: 28 January 2004
Accepted: 12 April 2004
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References |
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