Address correspondence to Ulrich H. von Andrian, CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115. Phone: (617) 278-3130; Fax: (617) 278-3190; email: uva{at}cbr.med.harvard.edu
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Key Words: homing intravital microscopy leukocyte adhesion leukocyte rolling vascular addressin
The best understood example of endothelium-controlled subset-specific leukocyte recruitment is the homing of lymphocytes to peripheral LNs (PLNs; 35). PLNs are strategically positioned to collect antigen via afferent lymphatics, which drain lymph from the periphery into the subcapsular sinus (1, 6). Professional antigen-presenting cells migrate via lymphatics from peripheral tissues into PLNs to interact with lymphocytes. B cells are concentrated within follicles in the PLN cortex, whereas T cells reside in the subcortex and paracortex. Most lymphocytes that home to PLNs and fail to encounter their cognate antigen leave the node by migrating through the medulla into efferent lymph vessels (7).
Blood vessels in different anatomic regions of PLNs possess distinct properties. Typical murine PLNs are supplied by a small artery that penetrates the capsule near the hilus and emits arteriolar branches toward the cortex (8). Arterioles give rise to capillary networks, which merge in the T cell area into high endothelial venules (HEVs) ranging in diameter from
Lymphocyte homing occurs mainly in high order HEVs (3, 4). The first step is the binding of L-selectin (CD62L) to endothelial sulfated carbohydrate (CHO) ligands. L-selectinmediated adhesion is characterized by high on-rate and tensile strength, allowing free-flowing lymphocytes to tether in the presence of shear. Tethered cells roll slowly downstream until they are stimulated by the chemokines CCL21 or CXCL12, which activate LFA-1 integrins whose engagement allows for firm arrest and diapedesis (35).
L-selectin is critical for lymphocyte homing to PLNs (10, 11). L-selectin ligands in HEVs are a heterogeneous set of glycan-based structures termed peripheral node addressin (PNAd), which are recognized by mAb MECA-79 (12, 13). The MECA-79 antigen is a core 1linked, 6-sulfated, sialyl-Lewisx (sLeX)-like, O-glycan that decorates several endothelial sialomucins, including CD34, GlyCAM-1, podocalyxin, and spg200 (1417). For L-selectin ligand function, PNAd must be sialylated, sulfated, and
Required enzymes for PNAd function in vivo are
Here, we provide evidence that LOVs in mouse subiliac LNs express functional L-selectin ligand(s) that are spatially and antigenically distinguishable from MECA-79reactive PNAd. The MECA-79reactive material is restricted to paracortical and subcortical venules (order IIIV), whereas L-selectin ligand(s) in medullary LOVs are MECA-79-. Moreover, the medullary L-selectin ligand activity is regulated largely by FucT-IV, which is more highly expressed in LOVs than elsewhere in PLNs.
L1-2 Cell Preparation and Labeling.
Flow Cytometry.
Animals.
Surgical Preparation of Subiliac LN.
Assessment of Intravascular Cell Behavior.
Cell behavior in PLN venules was assessed as previously described (3, 4, 8). The rolling fraction was determined as the percentage of cells that rolled along the vascular lining in the total flux of cells per venule. Rolling cells that arrested for at least 30 s were considered firmly adherent, and their number per luminal surface area was calculated for each venule. Velocity analysis was performed by frame by frame analysis of two representative vessels per experiment as previously described (8). Trajectories of individual cells were traced during passage through capillaries and venules, and their displacement from the point where they first entered the field of view was measured for every video frame. The instantaneous velocity of rolling cells, i.e., the displacement between subsequent video frames (1/30 s), was measured over up to 160 video frames and plotted as a function of time.
In Vivo Mapping of Antigen Densities in PLN Venular Trees.
Control experiments performed with control mAb-coated beads revealed no preferential accumulation in particular venular branching orders. To account for random and somewhat variable background binding, bound control NR beads and Ag-specific YG beads were enumerated throughout the entire PLN venular tree (NRPLN and YGPLN, respectively). For the specific accumulation of YG beads, YGsp was determined as YGsp = YGPLN- NRPLN, and the specific binding constant (c) was c = YGsp/YGPLN x 100 (%). From the density of adherent YG beads per luminal surface area in each individual venular segment, YGven, the specific venular concentration, [YGven], was calculated as: [YGven] = YGven x c (mm-2). The specific arteriolar concentration [YGart] was calculated accordingly.
Homing Experiments.
Sorting of PLN ECs.
Analysis of FucT Expression by RT-PCR.
Two pairs of primers were added for simultaneous amplification of cDNA pairs for FucT-IV, FucT-VII, and ß-actin. Primer sequences (5'-3') were as follows: FucT-IV sense: TGGACGCGTGGCGAGCCGCGGTGGCCACTCGTGGA, FucT-IV antisense: AACACGCGTAGTACCAGCGCCTTATCCGTGCGTTC; FucT-VII sense: CCTCTCTCTGGGCCCACATCCCCACTACCG, FucT-VII antisense: TCATAGACCTGGCTGCGGGGCAAGTAAGGG; and ß-actin sense: TTGGG-TATGGAATCCTGTGGCATCCATGAAAC, ß-actin antisense: TAAAACGCAGCTCAGTAACAGTCCGCCTAGAA.
PCR amplification was performed on five replicates/sample: step 1, 95°C for 15 min; step 2, 94°C for 1 min; step 3, 70°C for 1 min; step 4, 72°C for 1 min. Steps 24 were repeated 39 times. At the end of cycles 31, 33, 35, and 37, one replicate tube from each sample was incubated for 10 min at 72°C. Samples were run on a 4% acrylamide gel in TBE buffer containing ethidium bromide. Empirical analysis of digital images of amplified bands using Adobe Photoshop 6.01 software revealed that samples that underwent 37 amplification cycles yielded optimal results for comparison of relative band intensities. Band intensities were determined as the median channel number in intensity histograms in fixed size regions set over each band. The background fluorescence was determined for each lane and subtracted from specific band signals in the same lane. Relative signal intensity ratios were calculated by dividing the median intensity channel of the larger amplicon by that of the smaller one.
Statistics.
Online Supplemental Material.
Introduction
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Abstract
Introduction
Materials and Methods
Results and Discussion
References
Endothelial cells (ECs) in postcapillary and small collecting venules support adhesion and diapedesis of circulating leukocytes, whereas capillaries or arterioles in most organs are essentially nonadhesive. Venules in most lymphoid and some nonlymphoid organs constitutively express adhesion molecules, so-called vascular addressins, and chemokines normally not found in other vascular beds (1). Because vascular addressins are tissue specific, i.e., they are either by themselves or in combination unique to the specialized venular ECs in a particular organ, they regulate the magnitude and composition of leukocyte populations that enter that organ (1, 2). Although some molecular pathways for organ-specific homing of circulating leukocytes are well understood, the cellular and molecular mechanisms that induce and sustain endothelial specialization and vascular addressin expression are still largely unknown. 1550 µm. HEVs merge into collecting venules lined by flat endothelium in the medulla (9). From here, blood is channeled through hilar vessels into an extranodal vein. The entire venular tree can be visualized by intravital microscopy (IVM) of murine subiliac LNs (superficial inguinal) (8). This structure consists of up to five branching orders where the low order venules (LOVs) comprise a large collecting venule in the hilus (order I) and upstream branches in the medulla (order II and some order III). High order branches are HEVs constituting most order III and all order IV and V venules (8).
(1,3)-fucosylated (1822). Thus, PNAd is the result of HEV-specific posttranslational events involving several glycosyltransferases and at least one sulfotransferase.
(1,3)-fucosyltransferase (FucT)-IV, FucT-VII, and the sulfotransferase HEC-GlcNAc6ST (also called LSST or GST-3; 2022).
(1,3)-fucosylation is particularly essential because homing is abolished in mice that lack both FucT-IV and FucT-VII (22). Although FucT-VII is important for selectin ligand generation (21), homing in FucT-VII-/- mice is less affected than in FucT-IV+VII-/- mice, indicating that FucT-IV compensates partially for FucT-VII deficiency (22). Treatment of mice with mAb MECA-79 inhibits lymphocyte homing to PLNs (12). However, homing is only moderately reduced (
50%) in HEC-GLCNAC6ST-/- mice despite the lack of luminal MECA-79 reactivity in HEVs (20). Thus, MECA-79reactive PNAd may not be the only endothelial structure recognized by L-selectin in PLN venules.
Materials and Methods
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Abstract
Introduction
Materials and Methods
Results and Discussion
References
Antibodies and Reagents.
Antibodies to murine L-selectin (Mel-14), PNAd (MECA-79), sialyl-LewisX/A (HECA-452), ICAM-2 (mIc2/3c4; provided by T.A. Springer, CBR Institute for Biomedical Research), and human L-selectin (DREG-200) were purified according to standard procedures. FITC-conjugated antimouse or rat IgM and IgG (Zymed Laboratories) were used for flow cytometry. Tetramethylrhodamine-5-isothiocyanate (TRITC), 2' 7',-bis-(2-carboxyethyl)-5 (and 6) carboxyfluorescein (BCECF), rhodamine 6G, and fluorescent polystyrene microbeads were purchased from Molecular Probes. FITC-dextran was from Sigma-Aldrich.
Murine L1-2 preB lymphoma cells transfected with human L-selectin (L1-2L-selectin; reference 13) were grown in RPMI 1640 containing 10% FCS (cRPMI), 250 µg/ml mycophenolic acid, 12.5 µg/ml xanthine, and 1X hypoxanthine/thymidine (GIBCO BRL). For experiments, transfectants that expressed between 70,000 and 100,000 L-selectin molecules/cell were labeled with 2.5 µg/ml BCECF for 30 min.
To assess surface expression of L-selectin on L1-2L-selectin transfectants, cells were labeled with 20 µg/ml mAb DREG-200 followed by FITC-conjugated antimouse IgG, and analyzed using a FACScanTM flow cytometer (BD Biosciences). L-selectin expression was quantified as previously described (23).
Young adult FucT-deficient mice and WT littermates (C57B1/6 x 129SV) were used (21, 22). T-GFP transgenic mice were generated in our laboratory (24). Mice were kept in a viral antibody-free, specific pathogen-free barrier facility. Experimental procedures were approved by Animal Committees of both Harvard Medical School and The Center for Blood Research in accordance with National Institutes of Health (NIH) guidelines.
Mice were anesthetized by intraperitoneal injection of 1 mg/ml xylazine and 5 mg/ml ketamine. The left subiliac LN was prepared for IVM as previously described, and the right femoral artery was catheterized for retrograde injection of fluorescent dyes, cells, beads, or mAbs (8). The preparation was transferred to a customized intravital video microscopy setup (Mikron Instruments) equipped with water immersion objectives (Carl Zeiss MicroImaging, Inc.). Fluorescent events in the LN microcirculation were visualized by video-triggered stroboscopic epi-illumination (Chadwick Helmuth), recorded by a silicon-intensified target camera (VE1000SIT; Dage MTI), and stored on Hi8 video tape.
Endogenous leukocyte interactions with vascular endothelium were visualized by injection of 1 mg/ml rhodamine 6G (3). In some experiments, 100 µg mAb Mel-14 or MECA-79 (10 mg/kg body weight) were injected i.v., and leukocyte behavior was recorded 15 min thereafter. To visualize L1-2L-selectin transfectants, BCECF-labeled cells were injected as previously described (8). Luminal surface area of PLN microvessels was calculated from the diameter and length of individual vascular segments (assuming cylindrical geometry) after injection of 150 kD FITC-dextran.
Distribution of MECA-79+ and MECA-79- venules in PLNs was assessed by injecting CFSE-MECA-79 (1 mg/kg body weight), which leads to specific accumulation of fluorescent mAb in HEV within 1015 min (25). For semiquantitative assessment of endothelial surface antigen density and distribution in PLN microvessels, protein Acoated yellow green (YG; Ex/Em: 505/515 nm) and Nile red (NR; Ex/Em: 535/575 nm) fluorescent microspheres (1 µm diameter) were labeled with 80 µg/ml antirat IgG or IgM. YG beads were incubated with 80 µg/ml MECA-79, HECA-452, or antiICAM-2. NR beads were incubated with isotype-matched control mAb. Binding of NR beads in PLN microvessels was recorded 15 min after i.v. injection. After NR beads had disappeared from the circulation (within 30 min), an equivalent amount of YG beads was injected and binding was recorded in the same vessels.
Lymphocyte homing was studied using donor cells from T-GFP mice in which PLN-tropic (i.e., naive and central memory) T cells express GFP, whereas other leukocytes are GFP- (24, 26). 2 x 107 T-GFP PLN cells were injected i.v. into recipient mice. After 2 h, mice were killed and single cell suspensions were prepared from pooled bilateral subiliac, axillary, and brachial PLNs. The frequency of homed GFP+ T cells was determined by flow cytometry as previously described (4). In some experiments, donor cells were labeled with 30 µg/ml TRITC and homed B cells (GFP- TRITC+) and T cells (GFP+ TRITC+) were enumerated. To assess the role of MECA-79reactive and MECA-79nonreactive L-selectin ligands, three mice received i.v. injections of either 100 µg mAb Mel-14, 10 mg/kg mAb MECA-79, or sterile saline. 15 min later, animals received 2 x 107 T-GFP cells and PLNs were harvested 2 or 24 h thereafter. The number of homed GFP+ cells per 106 injected cells was determined for each recipient. Homing in mAb-treated mice was expressed relative to the control mouse that received the same donor cells.
PLNs (inguinal, axillary, brachial, cervical, and popliteal) were collected from 60 young adult C57Bl/6 mice. Single cell suspensions prepared after type II collagenase treatment (Worthington Biochemical Corp.) were depleted of leukocytes using anti-CD45 paramagnetic microbeads (MACS; Miltenyi Biotec). Unbound cells were washed and stained using antiCD45-CyChrome, antiMECA79-PE, antiP-selectinIg (BD Biosciences) followed by antihuman Fc-FITC (Caltag), and antiCD31-biotin (BD Biosciences) followed by streptavidin-ECD (Coulter Immunotech). CD45- CD31+ ECs from LOVs (MECA-79- P-selectin-Ig+), HEVs (MECA-79+ P-selectin-Ig+), and nonvenular ECs (MECA-79- P-selectin-Ig-) were sorted using an EPICS Elite ESP cell sorter (Coulter Corp.).
EC mRNA was isolated from sorted LOVs (7,630 cells), HEVs (11,500 cells), and nonvenular vessels (20,200 cells) using an RNeasy Mini Kit (QIAGEN). After digesting genomic DNA, cDNA was generated using oligo dT/dNTP mixture (PerkinElmer) with or without Superscript II Reverse Transcriptase (GIBCO BRL). cDNA corresponding to 1,000 cells/sample was used in each PCR reaction (Hot Star TAq kit; QIAGEN).
For statistical comparison of two samples, a two-tailed Student's t test was used. Multiple comparisons were performed using the Kruskal-Wallis test with Bonferroni correction. Differences were considered statistically significant when P < 0.05. Data are presented as mean ± SEM.
Video 1 illustrates the topography of MECA-79reactive L-selectin ligands in a mouse PLN. Videos 24 document the behavior of L1-2 cells in a PLN. Video 5 shows an animated three-dimensional reconstruction of serial confocal sections through a subiliac LN 24 h after treatment with mAb MECA-79 and adoptive transfer of green fluorescent T cells. Fig. S1 is a schematic diagram of the microvascular anatomy shown in Videos 24. Videos 15 and Fig. S1 are available at http://www.jem.org/cgi/content/full/jem.20030182/DC1.
Results and Discussion
Top
Abstract
Introduction
Materials and Methods
Results and Discussion
References
L-Selectin Ligands Are Expressed in Both MECA-79+ HEVs and MECA-79- LOVs.
Previous IVM observations of murine subiliac LNs illustrate a striking distinction between different segments of the venular tree with regard to reactivity with CFSE-labeled anti-PNAd mAb MECA-79 (25). After i.v. injection of fluorescent mAb, paracortical and subcortical postcapillary and small collecting venules (orders IIIV) were brightly stained, whereas most large collecting LOVs in the medulla (orders III) were not (Fig. 1
A). Because MECA-79reactive glycans are considered the critical L-selectin ligands in PLNs, LOVs should support little or no L-selectinmediated rolling. However, primary mouse lymphocytes and stably transfected L1-2L-selectin cells rolled in order I and II venules, albeit at a lower frequency than in high order venules (3, 8, 25, 27).
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MECA-79 staining ended abruptly in LOVs, seemingly between adjacent ECs. Loss of immunoreactivity often coincided with vessel bifurcations, but occasionally staining disappeared midway along an order II or III venule, unrelated to any other apparent anatomic features (Fig. 2 A). The rolling fraction of L1-2L-selectin cells was highest in order IV and V venules, decreasing further downstream as the rolling cells detached and rejoined the fast moving blood stream (8, 27). However, detachment occurred throughout the venular tree and did not coincide with the disappearance of MECA-79 staining. Indeed, L1-2L-selectin cells continued rolling as they passed from MECA-79+ to MECA-79- LOVs, without appreciable immediate changes in velocity (Fig. 2 B). L-selectinmediated rolling is relatively jerky (28), which was somewhat more apparent in MECA-79- than MECA-79+ venules (Fig. 2 C). However, as is typical for microvascular leukocyte behavior, there was considerable heterogeneity even within MECA-79+ venular segments because the immediate postcapillary venules supported smoother and slower rolling than most downstream venules.
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It should be cautioned that differences in bead accumulation between mAbs may not necessarily reflect different absolute densities of endothelial antigens. Factors including the number of functional binding sites per bead, mAb affinity, avidity, and accessibility of cognate epitopes are important. For example, MECA-79 and HECA-452 are multivalent IgMs recognizing CHO-capping groups in the endothelial glycocalyx, whereas antiICAM-1 and antiICAM-2 are bivalent IgGs that bind epitopes on the core of glycoproteins located close to the cell membrane. Nevertheless, this assay provides a relative measure of the intravascular accessibility and relative abundance of endothelial surface molecules in the microcirculation.
Because anti-ICAMcoated beads showed no statistically significant preference for any particular venular order, the skewedness in MECA-79 and anti-sLeX/A coated bead distribution toward high order venules cannot be explained by segmentally distinct microhemodynamics. Rather, the findings indicate that MECA-79reactive glycans are only expressed in high order venules and are absent from LOVs. sLeX/A has a similar distribution, but is expressed at a low level in order I venules.
MECA-79 Blocks L-Selectin Tethering Only in High Order PLN Venules.
Because LOVs are devoid of MECA-79 reactivity, but support L-selectin binding, we postulated that MECA-79 treatment should not affect rolling in LOVs. Saturating amounts of MECA-79 (10 mg/kg) nearly abolished rolling of L1-2L-selectin cells in high order venules, but reduced rolling in LOVs only slightly (Fig. 4
A). This partial inhibition by MECA-79 in LOVs was not necessarily caused by locally expressed MECA-79 antigen because most cells that rolled in LOVs initially tethered in upstream MECA-79+ segments (Fig. 2, B and C). If margination in high order venules is prevented by MECA-79, fewer cells are likely to interact downstream even if LOVs express different L-selectin ligand(s). Indeed, MECA-79 had no significant effect when only those L1-2L-selectin cells that were free flowing in high order venules and initiated rolling after entering LOVs were taken into account (Fig. 4 B).
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MECA-79independent L-Selectin Ligands Mediate T Cell Homing to PLNs.
We tested the relative contribution of MECA-79reactive and nonreactive L-selectin ligands to lymphocyte homing in PLNs. We used T-GFP mice in which PLN-tropic naive and central memory T cell subsets express GFP (4, 26, 30). Consistent with recent findings (31), treatment with MECA-79 abrogated T cell accumulation in WT PLNs at 2 h (Fig. 4 C). However, short-term homing may not be sufficiently sensitive to detect subtle contributions by other ligands. Indeed, after 24 h, PLNs in MECA-79treated recipients contained 83.7 ± 4.5% (mean ± SEM) fewer homed cells than in control mice, compared with 98.6 ± 0.2% inhibition with antiL-selectin (P < 0.05; n = 4 mice/group).
Serum MECA-79 decreased from 30 µg/ml at 2 h to 5 µg/ml at 24 h after a single injection. To increase the mAb concentration at 24 h, two mice received an additional injection of 10 mg/kg MECA-79 12 h after T cell transfer, resulting in 27 µg/ml MECA-79 at 24 h. Despite these different blood levels, T cell homing was equivalent (82 vs. 86% inhibition), indicating that both treatment regimens achieved maximal inhibition of homing.
T cells that homed to PLNs of MECA-79treated recipients were not trapped in microvessels but resided throughout the T cell area, some in the vicinity of LOVs (Fig. 4 D). Because homed T cells migrate rapidly and in random directions as soon as they have passed across HEVs (32), localization of homed cells may not necessarily reflect the microvascular segment in which they became initially adherent. Nevertheless, these data strongly suggest that a significant fraction of L-selectindependent T cell homing to PLNs is insensitive to MECA-79 inhibition.
L-Selectinmediated Rolling Has Distinct Requirements for FucT-IV and FucT-VII in High Order Venules and LOVs.
Although physiologic selectin ligands require (1,3)-fucosylation by FucT-VII and, to a lesser degree, by FucT-IV, the MECA-79 epitope is not fucosylation dependent (21, 22, 33, 34). Recent work has shown that MECA-79 epitopes are associated with core 1linked O-glycans whose intravascular expression depends on 6-sulfation of a GlcNAc residue by the HEV-specific sulfotransferase, HEC-GLCNAC6ST (17, 20, 31). Nevertheless, fucosylation is essential for L-selectin ligand function in PLN HEVs because lymphocyte homing is markedly reduced (by
80%) in PLNs of FucT-VII-/- mice, and the homing defect is significantly more severe in FucT-IV+VII-/- doubly deficient mice (22). To assess the role of fucosylation for L-selectin ligand(s) in LOVs, we compared the rolling behavior of rhodamine 6G-labeled circulating leukocytes in each venular order in PLNs of WT and FucT-deficient mice.
As expected, rolling was completely absent in FucT-IV+VII-/- PLNs, indicating that all L-selectin ligands are obligatorily (1,3)-fucosylation dependent (Fig. 5
A). However, the relative importance of FucT-IV and FucT-VII depended on anatomic localization. In high order venules rolling was predominantly dependent on FucT-VII. Some residual rolling was observed in HEVs of FucT-VII-/- mice, but not in doubly deficient PLNs, indicating a minor role of FucT-IV. This FucT-IVdependent ligand activity was blocked by MECA-79 treatment (Fig. 4 A and unpublished data). However, because MECA-79 is an IgM of relatively high molecular weight (12) and accumulates to high density in HEVs, we cannot exclude that FucT-IV generates L-selectin ligand(s) that are structurally distinct from the MECA-79 antigen, but still inhibitable by MECA-79 possibly due to steric hindrance.
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Together, these findings imply segmentally distinct enzyme requirements for venular L-selectin ligand generation. Although FucT-VII predominates in high order venules where the MECA-79 epitope is strongly expressed, FucT-IV prevails in LOVs because rolling was minimally affected in medullary venules of FucT-VII-/- PLNs. However, FucT-VII can also generate L-selectin ligands in LOVs because rolling was significantly enhanced in FucT-IV-/- LOVs, but completely lost in FucT-IV+VII-/- PLNs.
Naive T Cell Homing Is Enhanced in PLNs of FucT-IV-/- Mice.
Because FucT-IV deficiency unexpectedly boosted L-selectindependent rolling in LOVs, we sought to determine the physiologic significance of this observation for lymphocyte homing. Earlier studies using a mixture of naive and memory B and T cells from mesenteric LNs found a trend toward increased homing in FucT-IV-/- PLNs, but this was not statistically significant (22). Because different lymphocyte populations have distinct PLN tropism (30, 37), the inherent variability in homing of mixed lymphocyte populations might obscure subset-specific effects in FucT-IV-/- PLNs. Therefore, we stained T-GFP lymphocytes with the red fluorophore TRITC for subset-specific homing studies in PLNs of WT, FucT-IV-/-, or FucT-VII-/- recipients.
As predicted by our IVM studies and consistent with previous reports (21, 22), both GFP+ TRITC+ T cells (Fig. 5 C) and GFP- TRITC+ B cells (Fig. 5 D) homed poorly to FucT-VII-/- PLNs (P < 0.001). Strikingly, FucT-IV-/- PLNs contained 87% more donor T cells than WT PLNs (P < 0.05). Average B cell homing was also 67% higher in FucT-IV-/- PLNs, but this was quite variable (P > 0.05). Thus, FucT-IV exerts an attenuating effect on T cell homing to PLNs, presumably by reducing availability of L-selectin ligands in LOVs. On the other hand, homing is more severely compromised in FucT-IV+VII-/- PLNs than in FucT-VII-/- PLNs (22), indicating an additional adhesion-promoting role for FucT-IVdependent glycans, at least in FucT-VII-/- mice, which was also confirmed by our IVM studies. Given the prominent role of FucT-IV in L-selectin ligand production in LOVs, it seems likely that MECA-79insensitive L-selectin ligands in these vessels can contribute to the physiologic entry of lymphocytes into WT PLNs. Accordingly, we observed L-selectindependent, MECA-79insensitive T cell homing to PLNs in FucT-IV-/- mice, which was somewhat more pronounced than in WT mice (Fig. 4 C).
Selectin Ligand Activity in LOVs Is Transcriptionally Determined by the Balance of FucT-IV and FucT-VII Expression.
To explain why FucT-IV deficiency increases FucT-VIIdependent L-selectin ligand activity in LOVs, one must consider the acceptor preferences of FucT-IV and FucT-VII (38, 39). FucT-VII requires (2,3)-sialylated terminal lactosamine to generate sLeX-like selectin ligands. FucT-IV can also use the sialylated lactosamine substrate, albeit less efficiently than FucT-VII. However, unlike FucT-VII, FucT-IV additionally fucosylates internal GlcNAc residues in polylactosamine (38) and uses nonsialylated lactosamine to generate LewisX-like structures (22), which do not interact with selectins. Thus, FucT-IV may compete with
(2,3)sialyltransferase(s) and/or FucT-VII for terminal lactosamine as a shared precursor substrate on O-linked glycans. Consequently, FucT-IV may divert some terminal lactosamine acceptor moieties toward the Lewisx pathway that does not yield L-selectin ligands, and thus away from the
(2,3)sialyltransferase/FucT-VII synthetic route. In LOVs of WT mice, FucT-IV could thus attenuate FucT-VIIdependent production of sLeX-based L-selectin ligands. Without FucT-IV, increased acceptor availability for
(2,3)sialyltransferase/FucT-VII permits increased selectin ligand production, whereas FucT-VII deficiency might have lesser impact because sLeX-based L-selectin ligands could still be generated if FucT-IV is abundantly expressed.
Given these considerations, we hypothesized that venular L-selectin ligands in PLNs are regulated, in part, by the balance of FucT-IV and FucT-VII expression. FucT-VII is transcriptionally regulated by diverse external factors. It is induced postnatally in developing murine PLN HEVs. In adult PLNs, FucT-VII expression is transiently down-regulated upon antigenic challenge, and afferent lymph flow is essential for steady-state expression (40). Whether endothelial FucT-IV is also subjected to spatial and/or temporal regulation has not been examined.
Based on a combination of EC-specific antigenicity (CD45- CD31+) and differential selectin ligand expression, we sorted three distinct EC populations from PLNs (Fig. 6 A): (a) nonvenular MECA-79- ECs (from capillaries, some arterioles, and possibly lymphatics) that did not express selectin ligands, (b) MECA-79+ ECs from high order venules, and (c) MECA-79- ECs from LOVs expressing selectin ligands. To identify the latter, we exploited the fact that PLN venules support not only binding of L-selectin, but also P-selectin and E-selectin (27, 41). Indeed, both P-selectin+ activated platelets and L1-2P-selectin transfectants tethered and rolled efficiently in LOVs, but not in capillaries or arterioles (unpublished data). Thus, LOV ECs were defined as MECA-79- CD45- CD31+ cells that bound P-selectinIg chimeric protein. Substituting P-selectinIg for L-selectinIg facilitated this approach, because L-selectinIg yielded poor staining (unpublished data), presumably due to the shorter bond lifetime and/or the more pronounced requirement for fluid shear that distinguish L-selectin from P-selectin bonds (28, 42, 43).
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The results of this semiquantitative analysis, which was repeated twice with similar results, were in excellent agreement with our hypothesis. FucT-IV was present in all EC samples, but relative to ß-actin its expression in nonvenular endothelium was 27 and
6 times lower than in LOVs and high order venules, respectively. In contrast, FucT-VII was only detectable in venular ECs and, compared with ß-actin, FucT-VII mRNA was 3.6 times more abundant in high order venules than in LOVs. Strikingly, the FucT-IV/FucT-VII ratio was 5.7 times higher in LOVs than in HEVs.
Topography and Biochemistry of L-Selectin Ligands in PLNs.
Assuming that ß-actin expression is invariable in PLN ECs, a map of the relative expression levels of FucT-IV and FucT-VII can be generated (Fig. 7)
. FucT-IV predominates in LOVs where it may generate primarily nonsialylated fucosylated glycans, limiting production to one (or possibly more) sialylated L-selectin ligand(s) that is (are) antigenically distinct from the MECA-79 epitope. Although the precise structure of this molecule remains to be solved, the absence of rolling in FucT-IV+VII-/- mice shows a clear requirement for (1,3) fucosylation. In the absence of FucT-IV, activity of this ligand is enhanced possibly due to increased substrate availability for FucT-VII. However, FucT-IV probably dominates the physiologic ligand generation because FucT-VII deficiency had minimal impact on LOV adhesiveness.
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The sialomucin CD43 antagonizes L-selectindependent T cell tethering and rolling in PLNs (25). This antiadhesive effect is greater in LOVs than high order HEVs. This suggests that the ultrastructure and/or microenvironment of medullary L-selectin ligands differ from those in high order venules, thus allowing large, anionic surface molecules such as CD43 to exert increased antiadhesive activity in LOVs. Interestingly, FucT-VII modifies only glycoproteins, whereas FucT-IV also generates sLeX-containing glycolipids that support L-selectin binding in vitro (45, 46). Because rolling on glycolipids requires a closer proximity of surface membranes, it might be more sensitive to interference by CD43. However, whether FucT-IVdependent glycolipids can support in vivo L-selectin binding in LOVs or elsewhere remains to be determined.
Venular ECs in many nonlymphoid vascular beds express L-selectin ligands when exposed to inflammation (47, 48), including heparin-like molecules, which do not require fucosylation for functional activity (49). Moreover, expression of nonlymphoid endothelial L-selectin ligands is delayed (about 1 h) in response to inflammatory stimuli (50). Conversely, we observed rolling of endogenous leukocytes, isolated lymphocytes, and L1-2L-selectin cells in LOVs immediately after surgical preparation of PLNs (20 min after the initial skin incision), which was constant over several hours (unpublished data). Thus, although it cannot be excluded that the medullary L-selectin ligand(s) described here share(s) biochemical properties with L-selectin ligands elsewhere, its spatial restriction and constitutive expression in LOVs strongly suggest that this activity is unique to PLN medulla.
Although the medulla is arguably the least understood region in PLNs, its cellular composition is distinct from cortical regions. The medulla contains columns of macrophages, plasma cells, and T cells of mostly memory phenotype. It is assumed that these cells migrate to the medulla from other regions through the PLN parenchyma. Alternatively, some of these relatively rare leukocytes might be recruited via medullary venules, which we show here to constitute a specialized microvascular environment expressing at least one unique leukocyte traffic molecule. However, L-selectin alone would not be sufficient for leukocyte homing to the medulla because L-selectin allows cells only to roll, but not arrest. For this, an integrin-activating chemoattractant and endothelial integrin ligands are necessary. For example, in PLN HEVs, integrin activation on rolling T and B cells is mediated by CCL21 and CXCL12 (4, 5). Monocytes also home to PLNs that drain inflamed tissues via CCL2 transported to PLNs via afferent lymph vessels, and presented in HEVs to stimulate integrin-mediated monocyte arrest (51). Interestingly, our previous IVM observations using fluorescent mAbs to CCL21 or fluorescent CCL2 indicate that these chemokines are not tightly restricted to HEVs, but are present in medullary venules (unpublished data). Moreover, significant segmental differences were not revealed in expression of ICAM-1 and ICAM-2 within the venular tree (Fig. 3). Thus, all prerequisite components of multistep adhesion cascades are present in medullary venules. However, the magnitude, composition, and physiologic significance of cellular traffic through these microvessels must be further examined.
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Acknowledgments |
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This study was supported by NIH grants HL48675, HL54936, and HL56949 to U.H. von Andrian. C. M'Rini, R.T. Palframan, and E.J. Quackenbush were supported by stipends from NATO, the Wellcome Trust, and a Pfizer Scholars Grant from Children's Hospital, respectively.
Submitted: February 3, 2003
Revised: August 18, 2003
Accepted: September 24, 2003
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Footnotes |
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Abbreviations used in this paper: BCECF, 2' 7',-bis-(2-carboxyethyl)-5 (and 6) carboxyfluorescein; EC, endothelial cell; FucT,
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References |
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