By
From the Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20892-1360
Two cell permeable peptide fluoromethyl ketone inhibitors of Interleukin-1 converting enzyme (ICE) family proteases were tested as inhibitors of apoptotic cell death of T lymphocytes at various stages of differentiation. The CPP-32-like protease activity in apoptotic cell lysates
was blocked by both the ICE inhibitor Cbz-Val-Ala-Asp(OMe)-fluoromethyl ketone (ZVADFMK) as well as its truncated analog Boc-Asp(OMe)-fluoromethyl ketone (BD-FMK), which
failed to block ICE. In vitro apoptotic death in murine thymocytes triggered by the independent agents dexamethasone, etoposide, radiation, anti-Fas, and anti-CD3 was blocked equally well by BD-FMK and ZVAD-FMK, but not by the control reagent Cbz-Phe-Ala-fluoromethyl ketone. In activated T cell blasts, while anti-CD3/ Fas-induced death was almost completely inhibited by both ZVAD-FMK and BD-FMK, death induced by dexamethasone, etoposide, or irradiation was more sensitive to inhibition by BD-FMK. In the murine T cell line CTLL-2, apoptotic death induced by IL-2 withdrawal, etoposide, or dexamethasone was inhibited by BD-FMK, while ZVAD-FMK was without effect. These data indicate that ICEfamily proteases comprise a common functional step in distinct T cell apoptotic death pathways, but suggest that different family members are likely to be critical in various differentiated
T cell types, even when triggered by the same stimulus.
While programmed cell death has become recognized
as an important component of normal development
and immune function, the biochemical pathways leading
to such cell death remain poorly defined. However, the
recent demonstration that the nematode death gene ced-3
encodes a cysteine protease related to the mammalian interleukin-1 Convincing evidence for a functional role of ICE family
proteases in programmed cell death has come from several
strategies designed to selectively inactivate these proteases, particularly the expression of the virally encoded protein inhibitors CrmA and Baculovirus p35 (reviewed in reference
1). Peptide-based inhibitors of ICE family proteases have
also been shown to block apoptotic death in vivo and in vitro,
but their membrane permeability is sometimes a problem, and
their specificity has not always been adequately established.
We report here the ability of two newly developed cell
permeant peptide-fluoromethyl ketone inhibitors of ICE
family proteases to specifically block in vitro apoptotic death
processes in T lymphocytes triggered by different input
pathways. These results indicate that this protease family
comprises a common downstream step in apoptotic T cell
death pathways. The ICE inhibitor Cbz-Val-Ala-Asp(OMe)- fluoromethyl ketone (ZVAD-FMK) specifically blocks most
examples of T lymphocyte apoptotic death. However, several examples of T cell death which are resistant to ZVADFMK were blocked by the homologous inhibitor BDFMK, which blocks CPP-32-like proteases but not ICE.
These results suggest that for a single apoptotic stimulus,
different members of the ICE family are functionally important in different types of T cells, and show the use of
peptide-FMK reagents as probes of the role of ICE family
proteases in in vitro cell death systems.
Reagents.
The protease inhibitors Cbz-Val-Ala-Asp-(OMe)-
fluoromethyl ketone (ZVAD-FMK), Boc-Asp(OMe)-fluoromethyl ketone (BD-FMK), Cbz-Asp(OMe)-Glu(OMe)-Val-Asp
(OMe)-fluoromethyl ketone (ZDEVD-FMK), Cbz-Phe-Ala-fluoromethyl ketone (ZFA-FMK), Cbz-Ala-Ala-Asp-chloromethyl ketone (ZAAD-CMK) and the CPP-32 substrate Cbz-AspGlu-Val-Asp-7-amino-4-trifluoromethyl coumarin (ZDEVD-AFC)
were purchased from Enzyme Systems Products (Dublin, CA),
dissolved as stock solutions of 50 mM in DMSO, and stored at
Granzyme B Activity.
Granzyme B activity was measured in
detergent extracts of cloned murine CTL, provided by Dr. Martha Alexander-Miller (National Cancer Institute). Extracts were
prepared by treating 1 × 107 CTL with 1 ml of 1% Triton X-100
in assay buffer at 0°C for 10 min, followed by centrifugation at
11,000 g for 10 min. This extract was treated with the haloketone
reagents at the indicated concentrations for 2 h at room temperature, followed by the activity assay, which was carried out with
1 × 105 cell equivalents of extract/well in flat-bottom microtiter
plates using Boc-Ala-Ala-Asp-S-benzyl (BAAD-S-Bzl) (Enzyme
Systems Products) at a final concentration of 125 µM in 0.5 M
NaCl, 0.1 M Hepes, 1 mM EDTA, and 0.5 mM 5,5 CPP-32-like DEVDase Activity.
Jurkat cells (5 × 106/ml)
were incubated with 1 µg/ml CH-11 anti-Fas IgM on ice for 15 min, and then at 37°C for 45 min. After centrifugation, the cell
pellets were lysed in ICE buffer (1 × 106 cells in 200 µl 50 mM
Hepes buffer, pH 7.5, 10% sucrose, and 0.1% Triton X-100) for
20 min on ice. After centrifugation at 10,000 g for 10 min at 4°C,
200 µl of supernatant was transferred to a tube containing 2 µl 1 M
dithiothreitol. Peptide-FMKs were then added at the indicated
concentrations and incubated on ice for 15 min followed by
ZDEVD-AFC to a final concentration of 50 µM. After incubation at room temperature for 1 h, each tube was diluted with 2 ml
PBS, and fluorescence measured (excitation, 400 nm, emission,
505 nm). With the amount of cell extracts used, DEVD-ase activity was linear.
IL-1 Induction of T Cell Death.
T cells were obtained from C57Bl/
10 mice ( Jackson Laboratory, Bar Harbor, ME). T cell blasts
were generated by activating splenic T cells with PMA (2 ng/ml)
and ionomycin (1 µg/ml) for 48 h, followed by at least 24 h of
culture in complete medium (RPMI-1640 supplemented with
10% FCS, 100 IU penicillin, and 10 µg/ml streptomycin) with 10 U/ml of human recombinant IL-2 (Cetus Corp., Emeryville,
CA). CTLL-2 cells (American Type Culture Collection, Rockville, MD) were carried in complete medium supplemented with 2 U/ml IL-2. The protease inhibitors were added at the initiation of
200 µl cultures in all experiments described.
converting enzyme (ICE) has led to the identification of a family of cysteine proteases related by sequence homology (1). This ICE-family of proteases has an
unusual substrate cleavage specificity for aspartic acid residues at the P1 position. Studies of sequence homology and
fine specificity of substrate cleavage suggest there are two to
three subfamilies (2, 3): The ICE-like subfamily prefers substrates with hydrophobic amino acids at P4 (such as Tyr-ValAla-Asp [YVAD]), the CPP-32-like subfamily has less sequence homology to ICE and prefers substrates with acidic
amino acids at P4 (such as Asp-Glu-Val-Asp [DEVD]), and a potential ICH-1-like subfamily remains poorly characterized. In the case of death induced by Fas cross-linking, there is
evidence for a proteolytic cascade involving sequential activation of ICE-like enzymes and CPP-32-like enzymes (4, 5).
70°C. Fixed Staphylococcal aureus (Sansorbin) was obtained from
Calbiochem Corp. (La Jolla, CA). Polyclonal anti-human IL-1
was purchased from R&D Systems Inc. (Minneapolis, MN),
mouse anti-human Fas (CH-11) from Upstate Technologies Inc.
(Waltham, MA), and hamster anti-mouse Fas (Jo2) from PharMingen (San Diego, CA). Dexamethasone, etoposide (VP16), and
Hoechst 33342 were obtained from Sigma Chemical Co. (St.
Louis, MO). FITC-Annexin V was purchased from Brand Applications B. V. (Maastricht, Netherlands).
-dithiobis(2-nitrobenzoic acid) DTNB, pH 7.5. Activity was calculated
from initial slopes of plots of A414 versus time, relative to controls
with no inhibitor.
Processing.
IL-1 secretion from blood monocytes was
measured as previously described (6). Briefly, blood was preincubated with peptide-FMKs for one h, followed by addition of 50 µc/ml [35S]methionine and fixed S. aureus (Sansorbin; Calbiochem; 300 µg/ml). After 5 h of incubation at 37°C, supernatants
were immunoprecipitated with anti-IL-1
, and analyzed on 14%
nonreducing SDS gels.
-irradiation before culture. Immobilized antibodies were precoated on wells (5 µg/ml of 2C11
anti-CD3 or 10 µg/ml of anti-Fas) followed by washing. T cell blasts
(0.5 × 106/ml of IL-2 containing complete medium) were cultured with 0.1 µM dexamethasone, 2 µg/ml etoposide, and immobilized anti-CD3, or exposed to 5Gy irradiation before culture
to induce apoptotic death by 18 h. CTLL-2 cells (2.5 × 105/ml)
were cultured in IL-2 containing medium with 5 µg/ml etoposide or 50 µM dexamethasone for 18 h to induce death. For IL-2
withdrawal, cells were washed three times in complete medium
and cultured at 0.25 × 106 cells/ml in the absence or presence of IL-2.
Assays of Apoptotic Cell Death. To measure apoptotic nuclear morphology, cells were harvested and stained with 4 µg/ml Hoechst 33342 for 10 min at 37°C before fluorescence microscopy. Nuclei were scored as apoptotic if they displayed chromatin condensation and/or nuclear fragmentation. For propidium iodide staining, samples were counterstained with 5 µg/ml of propidium iodide. A minimum of 200 cells were scored for each data point. In some experiments membrane damage was quantitated by the trypan blue uptake of dead cells.
For analyzing the sub-G0 DNA content, cells were harvested by centrifugation and the cell pellets resuspended in lysis buffer (0.1% sodium citrate and 0.1% Triton X-100) containing 50 µg/ml of propidium iodide. Samples were stored at 4°C for 18 h before FACScan® analysis using CELL Quest software. To detect phosphotidylserine exposure on cell membranes, cells were stained with FITC-Annexin V (25 ng/ml) for 10 min at room temperature in the dark. Annexin V high cells were measured on a flowcytometer by gating on FSC/SSC settings for control untreated cells.Although appropriate peptide-chloromethyl ketones irreversibly inactivate both serine
and cysteine proteases, the less reactive fluoromethyl ketones are reported to be selective for cysteine proteases (7).
To confirm the selectivity of ZVAD-FMK and BD-FMK for cysteine proteases, we tested their ability to inactivate granzyme B, a serine protease recognizing asp-containing
peptides generally similar to those recognized by ICE family members. Fig. 1 A shows that these FMK reagents fail to
detectably inactivate granzyme B in CTL extracts at concentrations up to 400 µM. In contrast, ZAAD-CMK
blocks this enzyme, showing that peptide-asp-FMK reagents are selective for cysteine over serine proteases as expected.
Anti-Fas treated Jurkat T cells rapidly acquire a CPP32-like ability to cleave DEVD peptide substrates (8), and we have measured this activity in extracts of such cells using the fluorogenic substrate AcDEVD-AFC. Fig. 1 B shows the effect of peptide-FMKs on this activity. As expected, the homologous ZDEVD-FMK is a potent inhibitor, with ZVAD-FMK and BD-FMK also active with about 40 and 150 times less potency, respectively. In contrast, the control ZFA-FMK reagent lacking aspartate at P1 failed to show significant inhibition.
Fig. 1 C shows that ZVAD-FMK inhibits the conversion
of pre-IL-1 (31 kD) to active IL-1
(17 kD) in monocytes stimulated by S. aureus. The shorter reagent BDFMK is seen to be at least 100 times less potent in inhibiting this enzyme as is the control reagent ZFA-FMK, lacking
aspartate at the P1 position crucial for recognition by ICE
famliy proteases.
Thymocytes provide a well studied model
system for programmed cell death, which can be triggered
in vitro by four independent input pathways: corticosteroid, DNA damage by the DNA topoisomerase II inhibitor
etoposide or radiation, anti-Fas, and anti-CD3. Fig. 2 shows
the ability of ZVAD-FMK and BD-FMK to inhibit such
death. A shows that the spontaneous development of apoptotic nuclear morphology at 18 h is ~70% blocked by
100 µM ZVAD-FMK or BD-FMK, but not by an equal
concentration of ZFA-FMK. At this time, there is little
spontaneous death as judged by propidium iodide (PI) staining (B). Dexamethasone potently induces apoptotic
nuclear morphology, an effect blocked to control levels
by 50 µM ZVAD-FMK or BD-FMK, but unaffected by
ZFA-FMK. Cell death as assessed by PI (B) shows a similar
specific inhibition by both ZVAD-FMK or BD-FMK, but
somewhat more reagent is required to achieve levels of inhibition comparable to apoptotic nuclei. C-F show the
similar ability of peptide-FMKs to block thymocyte death by
etoposide and -irradiation. Previous studies have shown
both these death systems to be dependent on p53 (9). Thymocyte death induced by immobilized anti-Fas antibody
(Fig. 2, G-H ) is also effectively and specifically blocked by
ZVAD-FMK and BD-FMK, confirming the ability of other
ICE inhibitors to block anti-Fas triggered cell death (10,
11). Finally, immobilized anti-CD3 antibody induces somewhat less death in these cells than other stimuli (12), but
both death readouts again showed specific inhibition by
both ZVAD- or BD-FMK(I and J ). Thus, thymocyte death
triggered by four independent input pathways is specifically
inhibited by peptide-FMK inhibitors of ICE family proteases, with similar titration curves.
Diminished Blocking of T Lymphoblast Apoptotic Death by ZVAD-FMK, but not ZFA-FMK.
Upon activation, peripheral T cells acquire sensitivity to death by TCR crosslinking. Fig. 3 shows an experiment examining the ability
of peptide-FMK reagents to block T lymphoblast death induced by dexamethasome (A-C ), etoposide (D-F ), -irradiation (G-I ), and anti-CD3 ( J-L). The two independent
measurements of apoptotic nuclear damage gave very similar results (top two rows). As was the case for thymocytes, the
control ZFA-FMK reagent was without significant effect.
However, for these blasts, the inhibition patterns of ZVADFMK and BD-FMK are significantly different from those
found in thymocytes in several respects: (a) ZVAD-FMK shows clearly different inhibition patterns with different apoptotic stimuli, (b) BD-FMK is a generally better blocker of
apoptotic nuclear damage and cell death measured by
membrane integrity than is ZVAD-FMK, and (c) with dexamethasone and irradiation, BD-FMK prevented apoptotic
nuclear damage in some cells which nevertheless died as assessed by trypan blue. Although the latter comprise a minority of the cells which died, there is no evidence that their death occurred via a pathway dependent upon ICE
family proteases.
BD-FMK, but not ZVAD-FMK, Blocks Apoptotic Death in the IL-2-dependent CTTL-2 Cell Line.
Fig. 4 shows experiments examining the ability of ZVAD-FMK and BDFMK to inhibit apoptotic death in the IL-2-dependent
murine T cell line CTTL-2. In these experiments we also
measured the binding of FITC-Annexin V to the membrane, another nonnuclear apoptotic damage reflecting external phosphotidyl serine exposure (13). A previous report
showed that ICE inhibitors fail to block death mediated by
IL-2 withdrawal in this cell line (14). Fig. 4 confirms these
results with the ICE inhibitor ZVAD-FMK, which, like
ZFA-FMK, fails to block any of the death readouts induced by dexamethasone (A-D), etoposide (E-H ), or IL-2
withdrawal (I-L) in these cells. Unlike ZVAD-FMK, BDFMK inhibited CTLL-2 death with patterns quite similar
to those seen with thymocytes and lymphoblasts. Apoptotic
nuclear damage was completely inhibited by this reagent
while nonnuclear death criteria were in some cases less
completely blocked, reflecting the behavior seen with blasts.
While blocking in vivo and in vitro programmed cell death by expressing viral inhibitors of ICE family proteases has given convincing evidence of their general functional importance (1), such inhibitors are difficult to use experimentally to implicate these proteases in many important death systems. To probe T lymphocyte apoptotic death in vitro, we have studied two different peptide-FMK reagents designed to inactivate ICE family proteases. While further work is clearly needed to define the family members reactive with these reagents, the functional effects reported here allow insight into the changes in functional death pathways which accompany T cell differentiation. A combination of these experiments with future biochemical studies on ICE family proteases should lead to a more exact definition of molecular death pathways in T cells.
Both ZVAD-FMK and BD-FMK contain aspartate in the P1 position and are designed to irreversibly inactivate ICE family cysteine proteases in intact cells by alkylating the active site cysteine (7). Unlike other available peptide-based inhibitors of ICE family proteases, these FMK reagents can penetrate membranes readily because the aspartate carboxylate side chain is esterified and is expected to undergo cytoplasmic hydrolysis to the free aspartate derivative. Inactivation of a cellular process by a P1 asp-containing peptide-FMK reagent, but not a control reagent such as ZFA-FMK, shows that a member of the ICE family is functionally required, based on the unusual asp-peptide cleavage specificity of the ICE family and the selectivity of FMK reagents for cysteine proteases.
Because ICE-like proteases may become activated and in turn process CPP-32-like proteases (4, 5), it has been suggested that ZVAD-FMK inhibits death by selectively blocking the former (15). In support of this, 10 µM ZVADFMK was reported not to inhibit the ability of such CPP32-like proteases to cleave poly-ADP-ribose polymerase (15). However, the data of Fig. 1 suggest that ZVAD-FMK could block death by directly inactivating CPP-32-like members of the ICE family, and indicate that further studies with purified enzymes are required. Furthermore, Fig. 2 shows that the ICE inhibitor ZVAD-FMK and the nonICE inhibitor BD-FMK block thymocyte death about equally well, suggesting that ZVAD-FMK's ability to inhibit the ICE-like protease subfamily may not be the mechanism by which it blocks death. Cysteine protease inhibitors containing only a P1 asp have been previously shown to block some cell deaths (16, 17), but their reactivity with ICE family proteases remains to be established. In contrast to BD-FMK, a more reactive chloromethyl ketone analog blocks ICE (18). While more experiments are needed to assess the activity of BD-FMK with other ICE-family proteases, the results in Fig. 1 indicate that the ability of this reagent to block cell death could be due to its ability to directly inactivate some CPP-32-like ICE-family proteases.
The similarity of the inhibition titration curves of thymocyte death induced by the four independent input pathways tested in Fig. 2 is striking. In no case was significant inhibition seen with the control ZFA-FMK, indicating that asparatate at the P1 position is important. For apoptotic morphology, half maximal inhibition occurred at 15-40 µM ZVAD-FMK or BD-FMK, while comparable inhibition of death assessed by PI required about twice as much reagent. The similarity of the inhibition curves suggests that the same ICE family protease is the target of these reagents in inhibiting these four separate input pathways of thymocyte death.
Peptide FMKs inhibit the apoptotic death of activated peripheral T cells with a significantly different pattern than was found in thymocytes. ZVAD-FMK blocks Fas-induced death effectively, but has a variable effect on other death pathways in blasts. These experiments show several novel aspects of the role of ICE family proteases in T lymphocyte apoptotic death. For a single input pathway such as corticosteroid, T cell maturation is accompanied by a markedly diminished sensitivity to the ICE-inhibitor ZVAD-FMK, while sensitivity to BD-FMK is largely maintained. This is most readily explained by a shift in the particular ICE family protease members functional in the death pathway as the cells mature. However, in T cell blasts, the CD3-triggered Fas death pathway maintains its sensitivity to ZVAD-FMK, arguing that ICE-like target proteases are still expressed and accessible to the reagent. Thus the linkages between the different death signaling input pathways and ICE family proteases undergo interesting, and hitherto unappreciated, changes with differentiation of T cells.
The experiments with CTLL-2 in Fig. 4 show a more dramatic example of the different behavior of BD-FMK and ZVAD-FMK, with the latter completely ineffective in blocking any manifestation of death triggered by any death stimulus tested. These data are compatible with the findings of Vasilakos et al. (14) using other ICE inhibitors on the death of CTLL-2 cells by IL-2 withdrawal. However, our findings indicate that this is a property of CTLL-2 cells more than growth factor withdrawal, and our data with BDFMK indicate that other ICE-family proteases are part of all these death pathways. The behavior of CTLL-2 cells appears to be unusual among T cell death systems we have examined, and such ZVAD-FMK resistant apoptotic death processes may be uncommon generally, as this reagent and related ICE-specific inhibitors have been found to block a number of nonlymphoid in vitro and in vivo death systems (19).
The findings presented here make two related but distinct points: (a) they argue strongly that ICE family proteases comprise a common step(s) in the apoptotic death of most T lymphoid cells triggered by distinct input pathways, and (b) they show that death pathways in T cells at different stages of differentiation seem to use different ICE family members when death is triggered by the same input pathway. Many important issues remain to be elucidated regarding the functional ICE family proteases including the elements controlling their activation, interactions amongst family members, and their functional substrates leading to cell death. Our results show that peptide-FMK inhibitors of ICE family proteases permit simple functional experiments which can begin to shed light on some of these issues.
Address correspondence to Pierre A. Henkart, Bldg. 10, Rm 4B17, Experimental Immunology Branch, NCI, NIH, Bethesda, MD 20892-1690.
Received for publication 27 June 1996
We would like to thank Drs. Charles Zacharchuk and David Segal for their helpful comments on the manuscript.
1. | Henkart, P.A.. 1996. ICE family proteases: mediators of all apoptotic cell death? Immunity. 4: 195-201 [Medline] . |
2. |
Duan, H.,
A.M. Chinnaiyan,
P.L. Hudson,
J.P. Wing,
W.W. He, and
V.M. Dixit.
1996.
ICE-LAP3, a novel mammalian
homologue of the Caenorhabditis elegans cell death protein
Ced-3 is activated during Fas- and tumor necrosis factor-
induced apoptosis.
J. Biol. Chem.
271:
1621-1625
|
3. | Xue, D., S. Shaham, and H.R. Horvitz. 1996. The Caenorhabditis elegans cell-death protein CED-3 is a cysteine protease with substrate specificities similar to those of the human CPP32 protease. Genes Dev. 10: 1073-1083 [Abstract] . |
4. |
Chinnaiyan, A.M.,
K. Orth,
K. O'Rourke,
H. Duan,
G.G. Poirier, and
V.M. Dixit.
1996.
Molecular ordering of the cell
death pathway.
J. Biol. Chem.
271:
4573-4576
|
5. | Enari, M., R.V. Talanian, W.W. Wong, and S. Nagata. 1996. Sequential activation of ICE-like and CPP32-like proteases during Fas-mediated apoptosis. Nature (Lond.). 380: 723-726 [Medline] . |
6. | Thornberry, N.A., H.G. Bull, J.R. Calaycay, K.T. Chapman, A.D. Howard, M.J. Kostura, D.K. Miller, S.M. Molineaux, J.R. Weidner, J. Aunins, et al . 1992. A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes. Nature (Lond.). 356: 768-774 [Medline] . |
7. | Shaw, E.. 1990. Cysteinyl proteinases and their selective inactivation. Adv. Enzymol. Relat. Areas Mol. Biol. 63: 271-346 [Medline] . |
8. |
Schlegel, J.,
I. Peters,
S. Orrenius,
D.K. Miller,
N.A. Thornberry,
T.T. Yamin, and
D.W. Nicholson.
1996.
CPP32/apopain is a key interleukin-1-beta converting enzyme-like protease involved in Fas-mediated apoptosis.
J. Biol. Chem.
271:
1841-1844
|
9. | Clarke, A.R., C.A. Purdie, D.J. Harrison, R.G. Morris, C.C. Bird, M.L. Hooper, and A.H. Wyllie. 1993. Thymocyte apoptosis induced by p53-dependent and independent pathways. Nature (Lond.). 363: 849-852 . |
10. | Enari, M., H. Hug, and S. Nagata. 1995. Involvement of an ICE-like protease in Fas-mediated apoptosis. Nature (Lond.). 375: 78-81 [Medline] . |
11. | Los, M., M. Van de Craen, L.C. Penning, H. Schenk, M. Westendorp, P.A. Baeuerle, W. Dröge, P.H. Krammer, W. Fiers, and K. Schulze-Osthoff. 1995. Requirement of an ICE/CED-3 protease for Fas/APO-1-mediated apoptosis. Nature (Lond.). 375: 81-83 [Medline] . |
12. | Tadakuma, T., H. Kizaki, C. Odaka, R. Kubota, Y. Ishimura, H. Yagita, and K. Okumura. 1990. CD4+CD8+ thymocytes are susceptible to DNA fragmentation induced by phorbol ester, calcium ionophore and anti-CD3 antibody. Eur. J. Immunol. 20: 779-784 [Medline] . |
13. | Vermes, I., C. Haanen, H. Steffens-Nakken, and C. Reutelingsperger. 1995. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labeled Annexin V. J. Immunol. Methods. 184: 39-51 [Medline] . |
14. | Vasilakos, J.P., T. Ghayur, R.T. Carroll, D.A. Giegel, J.M. Saunders, L. Quintal, K.M. Keane, and B.D. Shivers. 1995. IL-1-beta converting enzyme (ICE) is not required for apoptosis induced by lymphokine deprivation in an IL-2-dependent T cell line. J. Immunol. 155: 3433-3442 [Abstract] . |
15. | Strasser, A., A.W. Harris, D.C.S. Huang, P.H. Krammer, and S. Cory. 1995. Bcl-2 and Fas/APO-1 regulate distinct pathways to lymphocyte apoptosis. EMBO (Eur. Mol. Biol. Organ.) J. 14: 6136-6147 [Abstract] . |
16. | Mashima, T., M. Naito, S. Kataoka, H. Kawai, and T. Tsuruo. 1995. Aspartate-based inhibitor of interleukin-1-beta converting enzyme prevents antitumor agent-induced apoptosis in human myeloid leukemia U937 cells. Biochem. Biophys. Res. Comm. 209: 907-915 [Medline] . |
17. | Boudreau, N., C.J. Sympson, Z. Werb, and M.J. Bissell. 1995. Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix. Science (Wash. DC). 267: 891-893 [Medline] . |
18. |
Estrov, Z.,
R.A. Black,
P.R. Sleath,
D. Harris,
Q. Van,
R. LaPushin,
E.H. Estey, and
M. Talpaz.
1995.
Effect of interleukin-1-beta converting enzyme inhibitor on acute myelogenous leukemia progenitor proliferation.
Blood.
86:
4594-4602
|
19. | Zhu, H.J., H.O. Fearnhead, and G.M. Cohen. 1995. An ICE-like protease is a common mediator of apoptosis induced by diverse stimuli in human monocytic THP.1 cells. FEBS Lett. 374: 303-308 [Medline] . |
20. |
Cain, K.,
S.H. Inayat-Hussain,
C. Couet, and
G.M. Cohen.
1996.
A cleavage-site-directed inhibitor of interleukin 1![]() |
21. | Pronk, G.J., K. Ramer, P. Amiri, and L.T. Williams. 1996. Requirement of an ICE-like protease for induction of apoptosis and ceramide generation by REAPER. Science (Wash. DC). 271: 808-810 [Abstract] . |
22. | Milligan, C.E., D. Prevette, H. Yaginuma, S. Homma, C. Cardwell, L.C. Fritz, K.J. Tomaselli, R.W. Oppenheim, and L.M. Schwartz. 1995. Peptide inhibitors of the ICE protease family arrest programmed cell death of motoneurons in vivo and in vitro. Neuron. 15: 385-393 [Medline] . |
23. | Jacobson, M.D., M. Weil, and M.C. Raff. 1996. Role of Ced3/ICE-family proteases in staurosporine-induced programmed cell death. J. Cell Biol. 133: 1041-1051 [Abstract] . |