Apoptosis of Fashigh CD4+ Synovial T Cells by Borrelia-reactive Fas-ligandhigh gamma delta T Cells in Lyme Arthritis

By Michael S. Vincent,* Karen Roessner,* David Lynch,Dagger David Wilson,* Sheldon M. Cooper,* Jurg Tschopp,§ Leonard H. Sigal,par and Ralph C. Budd*

From the * Divisions of Immunobiology and Rheumatology, Department of Medicine, The University of Vermont College of Medicine, Burlington, Vermont 05405-0068; Dagger  Immunex Corporation, Seattle, Washington 98101; § Institute of Biochemistry, University of Lausanne, Swiss Institute for Cancer Research, Epalinges, Switzerland; par  Division of Rheumatology and Connective Tissue Research, Department of Medicine, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903

Summary
Materials and Methods
Results
Discussion
Footnotes
Acknowledgements
References


Summary

The function of the minor subset of T lymphocytes bearing the gamma delta T cell antigen receptor is uncertain. Although some gamma delta T cells react to microbial products, responsiveness has only rarely been demonstrated toward a bacterial antigen from a naturally occurring human infection. Synovial fluid lymphocytes from patients with Lyme arthritis contain a large proportion of gamma delta cells that proliferate in response to the causative spirochete, Borrelia burgdorferi. Furthermore, synovial gamma delta T cell clones express elevated and sustained levels of the ligand for Fas (APO-1, CD95) compared to alpha beta T cells, and induce apoptosis of Fashigh CD4+ synovial lymphocytes. The findings suggest that gamma delta T cells contribute to defense in human infections, as well as manifest an immunoregulatory function at inflammatory sites by a Fas-dependent process.


While most T lymphocytes express a TCR composed of alpha  and beta  chains, a subpopulation of T cells bearing alternate gamma  and delta  chains exists as a minor subset of peripheral blood lymphocytes (PBL)1 (1). While the function of gamma delta T cells is uncertain, a clue may lie in their increased proportion at epithelial barriers, during certain infections, and at sites of chronic inflammation such as synovial tissue in rheumatoid arthritis (2). Some gamma delta T cells respond to bacterial products and can be identified after infection of mice with particular bacteria (8). However, in humans, leprosy is the only infectious disease to date in which gamma delta cells from affected individuals have been shown to respond to the causative organism (9).

gamma delta T cells frequently manifest cytolytic activity toward a broad array of target cells (2, 16). Such a spectrum of cytolysis might occur when a target molecule is widely expressed, such as the Fas antigen (APO-1, CD95) (17). Fas is a 45-kD cell surface molecule that mediates apoptosis and is a member of a family of molecules that includes the type I receptor for TNF. Fas is one of the principle components responsible for T cell-mediated cytotoxicity (18). Expression of mRNA for the Fas ligand (FasL) was originally described as being transiently expressed by activated alpha beta T cells, although higher mRNA levels were noted in gamma delta T cells (21). More recent findings have noted constitutive expression of FasL by nonlymphoid cells, including Sertoli cells of the testis (22) and certain components of the eye (23). FasL expression by these tissues parallels their ability to suppress immune-mediated inflammation. These collective observations suggested that gamma delta T cells in Lyme arthritis might respond to Borrelia burgdorferi as well as contribute to regulation of the synovial inflammatory infiltrate.


Materials and Methods

Patients. Lyme arthritis patients came from areas endemic for Lyme disease and were followed at the Lyme Disease Clinic at the University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School. All patients had histories, exams, and serologies consistent with Lyme arthritis, including Borreliaspecific antibody titers that were higher in synovial fluid relative to serum. Synovial fluid lymphocytes were examined from seven patients with Lyme arthritis of 6-mo to 3.2-yr duration.

Flow Cytometry. Lymphocytes were isolated from peripheral blood or synovial fluid by Ficoll-Hypaque centrifugation. Cells were stained with the indicated fluorochrome-conjugated antibody at 4°C for 30 min. Antibodies were specific for TCR-alpha beta (JOVI-1; Ancell Corp., Bayport, MN), TCR-gamma delta (5A6.E9; T Cell Sciences, Inc., Cambridge, MA), TCR-Vdelta 1 and TCR-Vdelta 2 (AB and BB3, respectively, courtesy of Dr. Alessandro Moretta, University of Genoa, Genoa, Italy), CD4 (SFCI12T4D11; Coulter Corp., Hialeah, FL), CD8 (SFCI21Thy2D3; Coulter Corp.), Fas (M38) (24) and FasL (polyclonal C-20; Santa Cruz Biotechnology, Santa Cruz, CA; or monoclonal A11 [25]). Surface staining for FasL was performed by one of three methods. The first approach used a fusion protein composed of the extracellular domain of murine Fas linked to the human Ig Fc portion (Fas-Fc) (26). This was followed by goat anti-human Fc-biotin and then avidin-phycoerythrin. Control staining was accomplished by staining for surface IL4 using an IL4 receptor-Fc fusion protein. Alternatively, surface FasL was measured using either a rabbit antiserum to the extracellular carboxyl-terminal portion of human FasL and purified on a FasL sepharose column (C-20), or monoclonal antibody A11 that recognizes both mouse and human Fas (25). To measure FasL induction, cells were examined 3 h after stimulation with PMA (10 ng/ml) and ionomycin (250 ng/ml), in the absence or presence of metalloprotease inhibition using 5 mM EDTA (27). Samples were analyzed on a Coulter Elite flow cytometer (Coulter Corp.) and at least 2 × 104 events were accumulated for analysis.

Proliferation Assays and Derivation of Lyme Synovial gamma delta T Cell Clones. Synovial fluid lymphocytes were cultured in AIM-V serum-free medium (GIBCO BRL, Gaithersburg, MD) in either bulk cultures (106/ml) for phenotyping, or in round-bottomed microtiter wells (105/well) for proliferation assays. Cells were stimulated with 3 µg/ml of a sonicate of B. burgdorferi grown in BSK II medium as previously described (28). Triplicate cultures were pulsed with 3H-TdR during the last 18 h of a 6-d culture, harvested, and counted. From parallel cultures, responding cells were cloned at 0.3 cells/well in AIM-V with 5% FCS in the presence of irradiated PBL (3 × 105/well), human recombinant IL2 (10 U/ml), and 3 µg/ml of B. burgdorferi sonicate. Responding wells were phenotyped and the gamma delta cells expanded by restimulation at 10-d intervals.

PCR Analysis of Synovial Fluid T Lymphocyte Vdelta Repertoire. Semi-quantitative PCR was performed on samples using cDNA prepared from oligo-dT-primed RNA and reverse transcriptase (GIBCO BRL) as previously described (29). The 5' Vdelta - and Cdelta -specific primers are modifications of published sequences (30) as follows: Vdelta 1: 5'-AGCAACTTCCCAGCAAAGAG-3'; Vdelta 2: 5'-AGGAAGACCCAAGGTAACACAA-3'; Vdelta 3: 5'-CACTGTATATTCAAATCCAGA-3'; Vdelta 4: 5'-TGACACCAGTGATCCAAGTTA-3'; Vdelta 5: 5'-CTGTGACTATACTAACAGCATGT-3'; Vdelta 6: 5'-TATCATGGATTCCCAGCC-3'; 5'Cdelta : 5'-CTTGTCTGGTGCAG-3'; 3'Cdelta : 5'-CTTCACCAGACAAGCGACAT-3'. A PCR reaction master mix that was common to all samples contained 100 mM Tris HCl, pH 8.3, 500 mM KCl, 2 mM MgCl2, 200 µM dNTPs, with 25 pmoles of 3' Cdelta primer, 2.2 µCi alpha -32P-dCTP, and 2.5 U Taq polymerase (GIBCO BRL) per tube. The final volume was 100 µl and contained 10 ng cDNA, and 25 pmoles of individual Vdelta primer. Samples were run on a thermocycler (model 9600; Perkin-Elmer Corp., Norwalk, CT) for 24 cycles using the parameters: cycle 1: 94°C × 3 min, 50°C × 45 s, 72°C × 1 min; cycles 2-23: 94°C × 30 s, 50°C × 45 s, 72°C × 1 min; cycle 24: 94°C × 30 s, 50°C × 45 s, 72°C × 7 min. Samples were resolved on a 29 cm 10% polyacrylamide gel containing 7 M urea in TBE buffer and electrophoresed at 80 V for 18 h. The gel was dried and developed on an analyzer (Betascope 603; Betagen, Waltham, MA). The percentage expression of each Vdelta was assigned by dividing the actual cpm for a specific Vdelta by the total cpm for Vdelta 1-Vdelta 6 after correction for the total Cdelta message in each sample.

Assay of Cytotolytic Activity. Faslow variants of the wild-type Jurkat T cell line, H7 (3% normal surface Fas levels) and B4 (1% normal Fas levels), were derived through irradiation mutagenesis using five doses of 200 Rads each, delivered at 5-d intervals. After each irradiation, cells were cultured in wells coated with lytic anti-Fas antibody (M2, 3 µg/ml)(24). The Faslow variants and wild-type Jurkat cells were incubated with 51Chromium (51Cr) for 1 h, washed, and then mixed at various effector/target ratios with cloned Vdelta 1 cells in a total volume of 200 µl. After a 4-h incubation at 37°C, 100 µl of supernatant were removed and counted for gamma  emission. Spontaneous release was determined from labeled targets in the absence of effector cells. Maximum release was determined by lysing target cells with 1.0 N HCl. The percentage of specific 51Cr release was calculated as:

%:Specific: <SUP>51</SUP>Cr::release:  :<FR><NU>experimental:cpm−spontaneous:cpm</NU><DE> maximal:cpm−spontaneous:cpm</DE></FR> .

Blocking studies of cytolysis were performed using either specific antibodies at the concentrations indicated, or Fas-Fc fusion protein (10 µg/ml) preincubated with appropriate cells for 30 min before beginning the cytolysis assay. Antibodies used were specific for TCR-gamma delta (5A6.E9), HLA class I (W6/32; Accurate Chemical and Science Corp., Westbury, NY), HLA class II (L243; Becton Dickinson & Co., Immunocytometer, Sys., Mountainview, CA), LFA-1 (R7.1; Biosource International, Camarillo, CA), or Fas (M38).

TUNEL Assay for Apoptosis. Cells were initially stained for expression of surface gamma delta , CD4, or CD8 and then fixed for 15 min in 1% paraformaldehyde. Cell membranes were then permeabilized for 15 min using 70% ethanol at 4°C. Samples were incubated at 37°C for 1 h in 100 µl containing 10 U terminal deoxyribosyltransferase and 0.5 nM dUTP-biotin (Boehringer Mannheim Biochemicals Corp., Indianapolis, IN) (31, 32). Specimens were washed twice with PBS/1% BSA and incubated with a 1:50 dilution of avidin-tricolor (Caltag Labs., South San Francisco, CA) at 4°C for 30 min. Cells were washed twice and analyzed by flow cytometry.


Results

Reciprocal Changes in Synovial Fluid CD4+ and gamma delta T Cells with Borrelia Stimulation.

Synovial fluid lymphocytes were examined from seven patients with Lyme arthritis of 6 mo to 3.2-y duration. These contained a predominance of CD4+ over CD8+ alpha beta T cells in only four of seven cases (Fig. 1 A, Table 1), compared to a consistent CD4 predominance in PBL. Also present in the synovial mononuclear cells was a remarkable percentage of gamma delta T cells (18.9 ± 6.8%) (Fig. 1 A, Table 1), compared to ~1-5% in PBL (Reference 1 and see Fig. 3). The synovial gamma delta population was largely devoid of surface CD4, and only a minor proportion (~20% on average) expressed low to intermediate levels of CD8 (Fig. 1 B). In addition, whereas gamma delta T cells from PBL express predominantly the Vdelta 2 gene product (33), Lyme arthritis synovial fluid gamma delta cells were primarily of the Vdelta 1 subset, with lesser proportions of Vdelta 2 and Vdelta 3 cells. This was determined by both flow cytometry using Vdelta -specific antibodies (Fig. 1 A), and semi-quantitative PCR using specific Vdelta primers (Fig. 2).



Fig. 1. Reciprocal shifts in the percentages of gamma delta versus CD4+ T cells after stimulation by B. burgdorferi of Lyme arthritis synovial fluid T cells. Synovial fluid mononuclear cells, isolated by Ficoll-Hypaque centrifugation, were analyzed either freshly isolated or 6 d after stimulation with a 3 µg/ml sonicate of B. burgdorferi. (A) Flow cytometric analysis of synovial fluid mononuclear cells reveals a prominent population of gamma delta cells that expresses mostly Vdelta 1 and expands dramatically following stimulation with B. burgdorferi. The numbers in the histograms indicate the percent of positively stained cells. (B) Synovial fluid gamma delta cells are predominantly CD4- CD8-. FACS® staining demonstrates that gamma delta cells are largely devoid of CD4 and only a minor subset expresses low to intermediate levels of surface CD8.
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Table 1. Phenotypic Changes in Lyme Arthritis Synovial Fluid T Cells following Borrelia Stimulation


Patient No. Before Borrelia stimulation After Borrelia stimulation Proliferation
%CD4/%CD8 %TCR-gamma delta %CD4/%CD8 %TCR-gamma delta Medium + Borrelia

cpm
1 29.7/45.3 23.8  9.0/39.8 56.8  5,316 178,964
2 52.6/28.2 20.0 18.0/29.6 45.8     526  19,122
3 27.8/45.7 15.7 29.0/33.2 29.9 17,369  82,440
4 64.4/16.6 11.1 55.1/8.8 11.8  2,791  32,418
5 25.4/39.5 13.5  7.4/38.3 41.5  4,516  51,278
6 36.5/18.8 30.8 51.4/28.9  2.9  2,937  44,474
7 45.4/35.3 11.6 14.0/23.6 25.7 30,230  89,533

Synovial fluid lymphocytes were isolated by Ficoll-Hypaque. Specimens were analyzed freshly isolated, or placed in serum-free AIM-V medium (GIBCO BRL) with a sonicate of B. burgdorferi (3 µg/ml) and re-phenotyped after 7 d. Proliferation of 5 × 104 cells/well was measured by 3H-TdR uptake during the last 18 h of culture. cpm, mean of triplicate cultures. Standard deviations were <15%.


Fig. 3. High level expression of surface Fas by synovial CD4+ cells. Mononuclear cells from freshly isolated Lyme arthritis synovial fluid or PBL were immunofluorescently stained for surface expression of Fas and either CD4, CD8, or gamma delta . Number insets represent percent of positively stained cells. Only the CD4+ subset of synovial fluid cells contains a large proportion of Fashigh cells.
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Fig. 2. Semi-quantitative PCR using 5' primers specific for Vdelta 1Vdelta 6 or Cdelta , and a 3' primer specific for Cdelta . (A) Actual polyacrylamide gel, imaged on a Betascope, of the Vdelta bands for Lyme synovial fluid mononuclear cells either freshly isolated (lane 1) or six days after culture with B. burgdorferi (lane 2). No Vdelta product was detected from PBL (lane 3) using the parameters of this assay. At the right side of the gel are the Cdelta products from the same three specimens. In addition, a control Cdelta product, C , is shown from a Vdelta 1 clone. (B) Graph of the quantitation of the Betascope results for the synovial fluid lymphocytes freshly isolated (SF 0') or after culture with B. burgdorferi (SF + Bb). Each Vdelta is displayed as a percentage of the total Vdelta cpm.
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Stimulation of Lyme arthritis synovial fluid mononuclear cells with a sonicate of B. burgdorferi (strain N40) induced vigorous proliferation (Table 1), yielding a two- to threefold increase in cell number over 6 d. During this period, the composition of T cell subsets shifted considerably. Although the percentage of CD8+ cells changed only slightly, there was frequently a striking loss in the proportion of CD4+ cells by as much as threefold. Thus, despite the increase in total lymphocyte number during the 6-d culture, there was frequently little change or even a decrease in the absolute number of CD4+ cells, as illustrated by patient no. 2 in Table 2. This was paralleled by a reciprocal increase in gamma delta T cells, in some cases to as much as 50% of the cultured synovial lymphocytes (Fig. 1, Table 1). These continued to be mostly Vdelta 1 cells as determined by both antibody (Fig. 1 A) and PCR (Fig. 2) analysis.

Table 2. Changes in Absolute Numbers of Synovial T Cell Subsets with Borrelia Stimulation


Fresh (total absolute No. = 1.1 × 106) + Borrelia (total absolute No. = 2.3 × 106)
Subset Percent Absolute No. (× 105) Percent Absolute No. (× 105)

CD4+ 52.6 5.8 18.0  4.1
CD8+ 28.2 3.1 29.6  6.8
 gamma delta + 20.0 2.2 45.8 10.5

Synovial fluid lymphocytes from patient No. 2 were analyzed for surface phenotype and absolute counts were determined when freshly isolated and after seven days of stimulation with B. burgdorferi.

The loss of CD4+ synovial cells might have resulted from unresponsiveness of this subset to B. burgdorferi, and hence overgrowth by the CD8+ and gamma delta + subsets. However, this seems unlikely since we have previously observed that PBL also proliferate strongly to B. burgdorferi with an expansion of predominantly CD4+ cells (28). Alternatively, because PBL contain only a small proportion of gamma delta cells (1), the gamma delta subset might be responsible for the loss of CD4+ cells in Borrelia-activated synovial cultures. Consistent with this notion was the one case (patient no. 6) where the percentage of gamma delta T cells did not increase following stimulation with B. burgdorferi. In this instance, the proportion of CD4+ cells actually increased from 36.5 to 51.4% (Table 1).

Synovial CD4+ Cells are Fashigh Whereas Synovial gamma delta T Cell Clones are FasLhigh.

To more directly address the possibility that synovial gamma delta cells might be cytolytic toward the CD4+ subset, gamma delta T cell clones were derived from synovial fluids of two Lyme arthritis patients using a sonicate of B. burgdorferi and irradiated autologous PBL. A panel of 18 Borreliaresponsive gamma delta clones was established, the majority of which express Vdelta 1 and lack surface CD4 and CD8. DNA sequencing of the delta  chain from seven clones confirmed that they all express Vdelta 1, but were otherwise each unique and contained varying degrees of N region diversity (Roessner, K., manuscript in preparation).

gamma delta T cells frequently manifest cytolytic activity toward a broad array of target cells (2, 16). Such a spectrum of cytolysis might occur when a target molecule is widely expressed, as is the case with the apoptosis-inducing molecule, Fas (17). As shown in Fig. 3, Fas expression by fresh CD4+ PBL was low to negligible, but was present on a large proportion of CD4+ synovial lymphocytes. By contrast, the CD8+ and gamma delta + subsets of PBL or synovial lymphocytes displayed considerably lower levels of Fas.

Surface expression of FasL protein by B. burgdorferi-reactive gamma delta and CD4+ alpha beta T cell clones was examined by flow cytometry using two methods, a Fas-Fc fusion protein as well as a purified anti-human FasL rabbit antiserum. Control staining for Fas-Fc was determined using a human IL4 receptor-Fc (IL4R-Fc) fusion protein (as surface-bound IL4 would not be anticipated for a secreted cytokine). Fig. 4 A (column 3) illustrates results of staining using the FasFc fusion protein, on represetative alpha beta (114B) and gamma delta (2.11) synovial T cell clones. By this method, surface FasL protein was expressed on a considerably higher proportion of the gamma delta cells than on the B. burgdorferi-reactive alpha beta T cell clones seven days after the last stimulation. Similar findings were seen with an additional two alpha beta and two gamma delta synovial T cell clones. In contrast, the levels of surface Fas antigen on the gamma delta clones were somewhat less than on the alpha beta clones, (Fig. 4 A, column 4).



Fig. 4. Lyme arthritis synovial gamma delta clones express high levels of FasL. (A) Surface FasL expression using a Fas-Fc fusion protein (column 3) is shown for a B. burgdorferi-reactive alpha beta T cell clone (114B) and a synovial gamma delta clone (2.11). Negative staining controls included either second step fluorescein-anti-Fc antibody alone (column 1), or initial staining with IL4R-Fc followed by second step anti-Fc antibody (column 2). Column 4 indicates the levels of Fas expression by the same clones. (B) Surface FasL expression detected by an anti-human FasL rabbit antiserum (C-20) purified using a FasL-sepharose column. Cell lines included the T cell leukemic line, molt 4, and the alpha beta and gamma delta T cell clones used in A. Shown is the control staining using rabbit immunoglobulin (   faint line) superimposed on the staining with the anti-FasL antibody (dark line). T cell clones were examined either seven days after the last stimulation with B. burgdorferi (unstimulated ), or 3 h after activation with PMA and ionomycin (iono). Cells were also analyzed in the absence or presence of the metalloprotease inhibitor EDTA in an effort to block degradation of surface FasL (27). Open numbers in histograms represent the percent of positive cells above background staining. Numbers in parentheses indicate the mean fluorescence intensity of the positively stained cells.
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The anti-FasL antibody confirmed the disparity in surface FasL expression between synovial gamma delta versus alpha beta T cell clones. Fig. 4 B (column 1) shows that 7 d after antigenic stimulation of the Borrelia-reactive alpha beta (114B) and gamma delta (2.11) clones, surface FasL was present on the gamma delta clone, but was only marginally detectable on the alpha beta clone. This finding was consistent for three alpha beta and three gamma delta clones studied. However, the alpha beta clones were capable of induction of FasL upon stimulation, as shown after 3 h of activation with PMA and ionomycin. In agreement with a recent report (27), FasL expression on the T cell line, Molt 4, was enhanced by blocking metalloprotease activity with EDTA (Fig. 4 B, column 4). This was less consistently observed for the alpha beta T cell clones, and was not observed for the gamma delta clones. It was particularly striking that the levels of FasL on the gamma delta clones remained detectable for at least 10 d following stimulation with B. burgdorferi (Fig. 4 B, column 1). This is in distinct contrast to alpha beta T cells which express FasL only transiently after activation (21; Roessner, K., unpublished observations).

Synovial gamma delta cells induce apoptosis of CD4+ cells in a Fasdependent manner.

To further explore whether the Lyme arthritis synovial fluid gamma delta T cell clones might be cytolytic toward T lymphocytes expressing high levels of surface Fas, the Jurkat T cell line was initially used as a representative Fashigh target. Fig. 5 A shows that the gamma delta clones manifested very efficient cytolytic activity toward Jurkat cells, with 50% maximal lysis achieved at an effector/target ratio between 10:1 and 3:1. This finding was remarkably consistent for each of five different Vdelta 1 clones tested from two patients. In contrast, Borrelia-reactive CD4+ alpha beta T cell clones manifested little, if any, cytolysis of Jurkat cells (data not shown). Cytolysis by the gamma delta clones was not inhibited by antibodies to TCR-gamma delta , HLA class I or II, but was blocked by anti-LFA-1 antibody (Fig. 5 C ), supporting the notion that cytolysis was dependent on cell contact.



Fig. 5. Lyme arthritis synovial fluid Vdelta 1 T cell clones are highly lytic in a Fas-dependent manner. Effector Vdelta 1 clones were combined at the ratios indicated with 51Crlabeled Jurkat target T cells in a four h cytolytic assay. (A) Comparison of cytolytic activity toward wild-type Fashigh Jurkat T cells (closed squares) compared with two Faslow Jurkat variants, H7 (open squares) and B4 (open circles), which express, respectively, 3 and 1% of surface Fas levels observed on wild-type Jurkat cells. (B) Level of Fas expression on wild-type Jurkat T cells and two variants, H7 and B4, selected by repeated irradiation and culture in the presence of lytic anti-Fas antibody, M2. Number insets indicate the mean fluorescence intensity of the gated area. (C ) Attempts to block gamma delta cytolytic activity using antibodies to HLA class I (closed squares), HLA class II (open squares), TCR-gamma delta (open circles), and anti-LFA-1 (closed triangles). The anti-LFA-1 study was part of a separate experiment in which the baseline cytolysis was 42%. (D) Ability of various concentrations of nonlytic anti-Fas antibody M38 to inhibit cytolysis of wild-type Jurkat cells by the Vdelta 1 clones. Cytolysis assay was also performed in the absence (closed squares) or presence (open squares) of 2.5 mM EGTA, an inhibitor of calcium-dependent perforin activity (18). Lysis in the presence of control IgG antibody (10 µg/ml) is shown by the closed triangle. (E ) Inhibition of Jurkat cytolysis by the Vdelta 1 clones 16 and 2.11 in the presence of 10 µg/ml of either anti-Fas antibody M38, Fas-Fc fusion protein, both, or IL4R-Fc fusion protein.
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The potential contribution of Fas to cytolysis by gamma delta cells was examined using three approaches. Initially, two Faslow variants of Jurkat cells, H7 and B4, were independently derived by radiation mutagenesis followed by selection with lytic anti-Fas antibody, M2. H7 expresses 3% of the levels of Fas found on wild-type Jurkat cells, whereas B4 displays 1% (Fig. 5 B). Fig. 5 A demonstrates that the efficiency of cytolysis of both Faslow variants was diminished approximately two- to threefold compared to that observed with wildtype Jurkat cells. However, lysis of the Jurkat Faslow variants was not completely eliminated, suggesting that part of the cytolytic activity of the gamma delta clones was independent of Fas. This was supported by anti-Fas antibody blocking studies.

Inhibition of Jurkat cell cytolysis by the gamma delta clones was also achieved using a nonlytic anti-Fas antibody, M38 (24). Fig. 5 D shows that the blocking of cytolysis with M38 was partial, achieving 30-50% inhibition at the highest concentration of antibody (10 µg/ml), whereas control mouse Ig did not block cytolysis. In vitro cytolysis consists of a calcium-independent component mediated by Fas and a calcium-dependent component delivered by perforin (18). Blocking perforin action by chelation of calcium with EGTA also resulted in partial inhibition of Jurkat cytolysis, which could then be blocked almost completely by the further addition of anti-Fas antibody (Fig. 5 D). A third method of disrupting Fas-FasL interaction used the Fas-Fc fusion protein. Fig. 5 E shows that Fas-Fc, but not IL4RFc, partially blocked cytolysis of Jurkat cells by the gamma delta clones, though to a slightly lesser extent than did nonlytic anti-Fas antibody.

The above findings show that gamma delta clones derived from synovial fluid express prolonged and high levels of FasL and suggest that gamma delta cells preferentially lyse Fashigh cells. To directly assess whether uncloned synovial gamma delta cells function in a similar manner, FasL expression was determined on synovial lymphocytes after Borrelia stimulation. As shown in Fig. 6 A, 7 d after activation, FasL expression was confined exclusively to a major proportion of the gamma delta cells. FasL was still expressed by at least 50% of the synovial gamma delta cells for as long as 11 d after Borrelia stimulation.



Fig. 6. Synovial gamma delta cells express FasL and their presence correlates with apoptosis of synovial CD4+ cells. (A) Lyme arthritis synovial fluid lymphocytes were stimulated with B. burgdorferi for 5 d and then stained with anti-gamma delta and monoclonal anti-FasL, A11. (B) Depletion of gamma delta cells from fresh Lyme arthritis synovial fluid was performed by flow cytometric sorting. Sorted and unsorted synovial fluid lymphocytes from the same specimen were then cultured with B. burgdorferi for 4 d and stained for expression of TCR-gamma delta , CD4, and CD8. Note the subpopulation of CD4low cells indicated by the white arrow insets, and the percent in that group noted by the number over the arrow. The CD4low cells comprise a larger proportion of the total CD4+ subset in the cultures containing gamma delta cells (gamma delta -nondepleted), even though they contained overall a smaller proportion of CD4+ cells compared to the gamma delta -depleted cultures. These findings were consistent in three experiments. (C) Determination of DNA fragmentation in a second gamma delta -nondepleted synovial culture using the TUNEL assay combined with surface labeling and flow cytometry. Note that the CD4low subpopulation is the subset undergoing apoptosis.
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To further assess the contribution of the gamma delta cells to the loss of synovial CD4+ cells, the gamma delta subset was depleted by flow cytometric sorting and compared to a nondepleted sample of the same specimen after five days of stimulation with B. burgdorferi. During this period, the gamma delta cells in the nondepleted synovial sample expanded from 4.3% to 11% (Fig. 6 B). This was accompanied by a decreased proportion of CD4+ cells, from 35.6 to 25.3%. In striking contrast, the gamma delta -depleted population contained only 4% gamma delta cells after 5 d and manifested a predominance of CD4+ cells (40.8%)(Fig. 6 B). In addition, the CD4+ cells in the 4-day cultures contained a subpopulation of CD4low cells which comprised a greater proportion of the total CD4+ cells in the gamma delta -replete than the gamma delta -depleted specimen (Fig. 6 B, arrow inset). These CD4low cells represented apoptotic cells, as determined by the TUNEL assay combined with surface staining and analyzed by flow cytometry (Fig. 6 C ). Smaller proportions of apoptotic cells were also observed in the CD8+ and gamma delta + subsets. Observations similar to these have been made with gamma delta depletion of two additional Lyme synovial fluid specimens, as well as by noting a depletion of CD4+ cells when Vdelta 1 cloned T cells were added to cultures of PBL that have been stimulated with B. burgdorferi (data not shown).

To assess whether the appearance of the apoptotic CD4low subset in the gamma delta -replete cultures was in part Fas-mediated, FasL was blocked using the Fas-Fc fusion protein. Synovial fluid lymphocytes were stimulated with B. burgdorferi in the presence of either no additives, Fas-Fc, or control mouse IgG. As shown in Fig. 7, the appearance of apoptotic CD4low cells occurred beginning about five days after Borrelia stimulation. The proportion of this subset increased dramatically thereafter in all cultures except that containing Fas-Fc. The findings support the view that the gamma delta subset induces apoptosis of synovial CD4+ cells at least partly through Fas/FasL interactions.


Fig. 7. FasL inhibition prevents appearance of CD4low apoptotic subset. Lyme arthritis synovial fluid mononuclear cells were stimulated with B. burgdorferi (B.b.) in the presence of no additives (closed circles), or 10 µg/ml of either Fas-Fc (closed squares), or murine IgG (open circles). After the indicated day of stimulation, cultures were analyzed for expression of CD4, CD8, and gamma delta . Shown is the expression of apoptotic CD4low cells (as identified by TUNEL in Fig. 6 C ) as a percentage of the total CD4+ cells. The results are representative of two experiments.
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Discussion

The collective observations suggest an immunoregulatory circuit whereby synovial Vdelta 1 T cells bearing high levels of FasL selectively restrict the expansion of infiltrating inflammatory Fashigh CD4+ lymphocytes through cytolysis in a Fas-dependent manner. The findings are in agreement with recent studies showing that FasL mRNA expression by T cells is highest in the gamma delta subset (21). Not only were levels of surface FasL high on the Vdelta 1 clones, they remained elevated for considerably longer periods than similarly activated alpha beta T cells. This may serve to explain the broad spectrum of cytolytic activity that has frequently been observed for many gamma delta cells (2, 16). The results parallel other recent descriptions of immunosuppression resulting from constitutive expression of FasL by Sertoli cells in the testis (22), and by components of the eye (23).

The current findings may also bear on observations that collagen-induced arthritis in mice (34) and adjuvant arthritis in rats (35) are both more severe following administration of anti-gamma delta antibody. Collagen-induced arthritis is also more aggressive in mice bearing a genetic deletion of the delta  locus (Lefrancois, L., personal communication). Similar results have been observed in a model of orchitis in which gamma delta depletion accelerated the inflammatory response (36). gamma delta T cells have also been observed to modulate the functional profile of CD4+ cells. In certain instances this has manifested as selectively inhibiting TH2-dependent cytokine responses, such as IgE production in an allergy model (37) and Coxsackievirus-induced myocarditis (38). The resulting TH1 bias may be due solely to the production of the TH1-type cytokine, IFNgamma , by gamma delta cells (37), but may also reflect a greater sensitivity of TH2 cells to Fas-mediated apoptosis. In this regard, it is noteworthy that B. burgdorferi- reactive CD4+ T cells from Lyme arthritis patients express a TH1 cytokine phenotype (39). Studies are in progress to determine whether a TH1 enrichment results in the residual CD4+ synovial T cells following stimulation with B. burgdorferi.

Lyme arthritis synovial gamma delta T cells also represent a rare instance where gamma delta T cell clones obtained from a human infectious disease manifest a proliferative response in the presence of the causative agent. This does not establish that Lyme arthritis synovial gamma delta cells are responding directly to a Borrelial component. It is entirely possible that B. burgdorferi induces the appearance of surface molecules to which Vdelta 1 cells respond secondarily. Cutaneous lesions in leprosy also contain gamma delta T cells that react to the causative agent, Mycobacterium leprae (9). The repertoire of gamma delta cells that react to mycobacterial products is restricted in both humans and mice (11, 40), and in some instances involves recognition of nonpeptide components such as prenyl pyrophosphates (15, 41). Conceivably, gamma delta cells in Lyme arthritis may also recognize nonprotein components of B. burgdorferi. On balance, the current findings are consistent with the concept that gamma delta cells participate in the defense against infectious agents while modulating the immune response through Fas-mediated apoptosis.


Footnotes

Address correspondence to Dr. Ralph C. Budd, Division of Immunology, The University of Vermont College of Medicine, Given Medical Building Room C-303, Burlington, VT 05405-0068.

Received for publication 22 May 1996

   This work was supported by National Institutes of Health grant AR43520 and the Arthritis Foundation.
   1Abbreviation used in this paper: PBL, peripheral blood lymphocytes.

We thank Colette Charland (The University of Vermont College of Medicine, Burlington, VT) for assistance with flow cytometry and Roberta Christie (The University of Vermont College of Medicine, Burlington, VT) for preparation of the manuscript.


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Copyright © 1996 by The Rockefeller University Press.