By
From the * Molecular Development of the Immune System Section and Lymphocyte Biology Section,
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of
Health, Bethesda, Maryland 20892
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Abstract |
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Activation, anergy, and apoptosis are all possible outcomes of T cell receptor (TCR) engagement. The first leads to proliferation and effector function, whereas the others can lead to partial or complete immunological tolerance. Structural variants of immunizing peptide-major
histocompatibility complex molecule ligands that induce selective lymphokine secretion or anergy in mature T cells in association with altered intracellular signaling events have been described. Here we describe altered ligands for mature mouse CD4+ T helper 1 cells that lead to
T cell apoptosis by the selective expression of Fas ligand (FasL) and tumor necrosis factor
(TNF) without concomitant IL-2, IL-3, or interferon production. All ligands that stimulated
cell death were found to induce FasL and TNF mRNA expression and TCR aggregation
("capping") at the cell surface, but did not elicit a common pattern of tyrosine phosphorylation of the TCR-associated signal transduction chains. Thus, TCR ligands that uniquely trigger T
cell apoptosis without inducing cytokines that are normally associated with activation can be
identified.
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Introduction |
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Tcell receptor (TCR) engagement can lead to several different responses in mature peripheral T lymphocytes, including activation and proliferation, anergy, and programmed cell death (apoptosis). These events are controlled by signals resulting from interaction of the TCR and CD4 or CD8 coreceptors with peptide-MHC complex ligands and contribute to the quality and extent of the T cell response. Structural variants of a TCR ligand can selectively affect individual effector functions and biochemical events in T cells (1). Introduction of amino acid substitutions in the peptide component of an agonist ligand for a given T cell may variously result in the formation of: (a) a nonagonist that elicits no T cell effector function; (b) a weaker or stronger agonist that elicits all T cell effector functions with a different dose response from the original ligand; (c) a partial agonist with the capacity to induce a subset of effector functions or anergy in T cells; or (d) an antagonist that diminishes the response to agonists. The discovery of these functional classes of variant TCR ligands promoted the notion that T cells can sense the "quality" as well as the "quantity" of TCR engagements (1). This concept has been strengthened by recent evidence for unique patterns of early tyrosine phosphorylation events induced by variant TCR ligands that cannot be seen in response to agonists (2, 3, 6).
The activation state of a T cell also dictates the outcome
of interactions between the TCR and the peptide-MHC
ligand. Agonist ligands stimulate lymphokine production
and proliferation in resting T cells, whereas in cycling cells,
they cause lymphokine production followed by death via
apoptosis (7, 8). The differential signaling capacity of the
TCR prompted us to investigate whether variant ligands
that selectively induce T cell death without effector activation might exist. Such ligands could be useful for dissecting the signaling pathways leading to apoptosis as well as for
developing effective immunotherapeutic agents. We therefore screened numerous variants of the pigeon cytochrome
c (PCC)1 88-104 peptide for the ability to trigger apoptosis
in an activated murine CD4+ T cell clone against PCC/I-Ek without inducing activation cytokines such as IL-2, IL-3,
or IFN-. We have identified altered TCR ligands with
selective death-inducing activity and report the initial characterization of their effects on activated T cells.
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Materials and Methods |
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T Cell Clones and APCs.
CD4+ A.E7 Th1 cells (9) were maintained by biweekly stimulation with PCC protein (5 µM) and irradiated splenocytes from B10.A (H-2a) mice. A.E7 cells stimulated in this manner 2 d previously were ficolled and transferred to fresh medium containing IL-2 (50-100 IU/ml recombinant human IL-2 (Chiron, Emeryville, CA) or 10-15% T-StimTM (Collaborative Biomedical Products, Bedford, MA) and incubated for an additional 2-5 d to generate cycling A.E7 cells that are predisposed to apoptosis after TCR engagement (8). P13.9, L cell transfectants expressing I-Ek, intercellular adhesion molecule 1 (ICAM-1), and B7.1 (CD80) molecules, were used as APCs (3).Peptides.
PCC (88-104) is KAERADLIAYLKQATAK. Peptides Y99, C99, R99, A99 are synthetic peptides in which the primary TCR contact residue, lysine in position 99, is changed to tyrosine, cysteine, arginine, or alanine, respectively. All peptides were synthesized by the Peptide Synthesis Facility, NIAID, National Institutes of Health [NIH], Bethesda, MD.Cell Death Assays.
5 × 104 P13.9 cells were incubated with 5 × 104 cycling A.E7 cells in the presence of various concentrations of PCC peptides in quadruplicate in 96-well round bottomed plates. To block apoptosis, Fas-Fc and TNFR-Fc (10 µg/ml; gift from Dr. David Lynch, Immunex, Seattle, WA) were included in some assays. Cells were harvested after 24 h of incubation, two of each four wells were pooled, and 10 µg/ml of propidium iodide were added. Ungated cells were acquired for 30 s at a constant flow rate using a FACScan® equipped with CellQuest software (Becton-Dickinson, Mountain View, CA) and the number of viable A.E7 cells in each sample was calculated as previously described (10, 11). In some experiments, P13.9 cells were preloaded with 0.1 µM carboxyfluorescein diacetate-acetylester (CMFDA; Molecular Probes, Inc., Eugene, OR) to allow APCs to be excluded from the analysis by gating on fluorescein-negative cells. In other experiments, A.E7 cells were stained with fluoresceinated anti-CD4 before flow cytometry to differentiate them from APCs.Cytokine Measurement.
Supernatants from the apoptosis assays were harvested at 24 h and assayed for cytokine production by ELISA. IL-2 and IL-3 were detected using antibodies purchased from PharMingen (San Diego, CA), essentially as per the manufacturer's instructions. IFN-mRNA Analysis by Semiquantitative Reverse Transcriptase PCR.
A.E7 cells were incubated with P13.9 cells prepulsed for 2 h with 100 µM of the indicated peptides and total RNA was isolated using RNAzol according to the manufacturer's instructions (Tel-test, Inc., Friendswood, TX). The reverse transcriptase (RT) reaction was performed using random hexamer primers and part of each sample was subjected to PCR amplification usingProtein Biochemistry.
2-5 × 106 P13.9 APCs were incubated for 2-4 h in 200 µl complete medium alone (no Ag) or with the indicated peptides at 100 µM. Pulsed APCs were then centrifuged together with 1-1.25 × 107 cycling A.E7 cells in Eppendorf tubes and warmed to 37°C, as described (6). After stimulation, T cells + APCs were lysed (6) and lysates were then subjected to immunoprecipitation with anti-CD3Fluorescence Microscopy.
106 P13.9 APCs were incubated for 2-4 h in 200 µl complete medium alone (NP) or with the indicated peptides at 100 µM. Pulsed APCs were then centrifuged together with 106 cycling A.E7 cells in Eppendorf tubes and warmed to 37°C for 5 min. Cells were incubated on ice for 5 min and an additional 15 min in presence of 10 µg/ml of anti-CD3 Ab (PharMingen), washed twice with cold PBS, and fixed for 30 min at room temperature in 1% PFA. For the detection of actin polymerization, A.E7 cells were similarly stimulated with APCs and different variant peptides. Cells were fixed for 30 min at room temperature in 1% PFA, washed twice in PBS 1% FCS and incubated 30 min in PBS/0.1% saponin/1% FCS/Texas red conjugated-X phalloidin (2 U/ml). Cells were washed twice with PBS and were mounted on slides using Fluoromount-GTM (Electron Microscopy Sciences, Fort Washington, PA). The images were obtained on a Zeiss Axiophot (Carl Zeiss, Inc., Thornwood, NY) with a CCD camera (Princeton Instruments, Inc., Trenton, NJ). ![]() |
Results |
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To
determine whether variant ligands can selectively elicit apoptosis responses, we screened many peptides with single
amino acid substitutions at the major epitopic residue
(lysine 99) of the agonist 88-104 peptide of PCC (PCC
88-104) for their effect on A.E7 cells that were actively cycling under the influence of IL-2. Certain variants of PCC
88-104 with substitutions at position 99 have been previously found to induce variant signaling in unactivated, resting A.E7 cells (3). These substitutions do not affect binding to the I-Ek molecule and therefore are assumed only to alter TCR recognition of peptide-MHC ligand (3, 14). In
our study of cycling A.E7 cells, we observed that the WT
PCC 88-104 peptide induces both cell death and the secretion of IL-2, IL-3, and IFN- (Fig. 1 A). In contrast, at
high concentrations, two of the variant peptides tested,
Y99 and C99, induce as much cell death as the WT peptide, but fail to induce IL-2, IL-3, or IFN-
production
(Fig. 1 A). T cell death under these circumstances has the
morphological and nuclear fragmentation characteristics of
apoptosis (8 and data not shown). Other variant peptides,
such as A99 and R99, do not stimulate either apoptosis or
lymphokine secretion (Fig. 1 A). To address the possibility
that the selective cell death induction by Y99 and C99 was
due to weak stimulation of all effector functions, we compared dose-response curves for apoptosis and lymphokine responses. The apoptosis assay is slightly more sensitive
than the cytokine ELISAs (Fig. 1 B); however, 50% maximal apoptosis requires ~1 nM WT ligand (Fig. 1 B) or 1 µM Y99 (Fig. 1 D). Because half-maximal cytokine production in response to the WT peptide requires at most 10-fold more agonist than half-maximal apoptosis, cytokine
production should be seen with 10 µM Y99 assuming the latter simply behaves as a weak agonist. Yet 100 µM of
Y99 or C99 causes no production of IL-2, IL-3, or IFN-
(Fig. 1 D and data not shown). We conclude that Y99 and
C99 are true partial agonists for A.E7 cells, because they induce apoptosis but not other typical effector functions. By
contrast, R99 and A99 fail to induce these responses at any
dose and are therefore nonagonists with respect to the responses we have examined (Fig. 1 C and data not shown).
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Because T cell apoptosis can result from
the action of Fas or TNF (15), we assessed the role of
these molecules in cell death induced by the Y99 and C99
partial agonists. Stimulation of cycling A.E7 cells for 2 h
with the apoptogenic WT, Y99, or C99 peptides induced
the mRNAs for TNF and Fas-L but not for Bcl-X, a protein that prevents apoptosis (19; Fig. 2 A). There was less induction of these mRNAs with the variants compared to
the WT peptide, in accordance with the latter's greater lethality at low concentrations (Fig. 1). Fas-L or TNF
mRNA levels were not increased using the nonagonist
peptides A99 and R99 (Fig. 2 A). Both Fas-L and TNF
were functionally important in the apoptosis caused by the
WT, C99, and Y99 peptides because death was potently inhibited by reagents that specifically block these cytokines (Fig. 2 B). In contrast to the WT peptide, none of the variants elicited IL-2 (Fig. 2 A) or IFN- mRNA (data not
shown), confirming the results obtained by ELISA (Fig. 1).
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In resting T cells, partial agonist or antagonist ligands generate
unique patterns of protein tyrosine phosphorylation (2, 3,
6). Hence, we determined whether apoptosis-inducing variant peptides produce characteristic TCR-associated phosphorylation patterns in cycling T cells. CD3-, ZAP-70, or TCR-
(Fig. 3, A, B, and C, respectively) and their associated proteins were immunoprecipitated from lysates prepared from
A.E7 cells that had been briefly stimulated with WT and
variant ligands. In cycling A.E7 cells, the WT peptide induced prominent tyrosine-phosphorylated species, including the three isoforms of phospho-
(p18, p21, and p23),
phosphorylated CD3-
, and phosphorylated ZAP-70 (Fig. 3, A and B, lane 2). This is the same pattern as that obtained when resting A.E7 cells were stimulated with the
WT peptide (3). However, we found no correlation between the pattern of TCR-
tyrosine phosphorylation and
programmed cell death. The apoptosis-inducing partial agonist, C99, and the nonagonist ligands, A99 and R99, all
produced a modest increase in the p21 form of phospho-
(Fig. 3, A-C, lanes 3-5). However, the partial agonist Y99
surprisingly failed to induce a detectable increase in p21
phospho-
, even upon examination by direct anti-
immunoprecipitation (Fig. 3, A-C, lane 6). None of the variant
ligands induced detectable levels of the other species of
phospho-
(p18 and p23), phosphorylated CD3-
, or phosphorylated ZAP-70 (Fig. 3, A and B, lanes 3-6).
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Successful activation of resting T cells with an
agonist peptide causes the redistribution of the TCR into
polarized focal aggregates on the membrane, known as
"capping" (20). We examined by fluorescent microscopy
the ability of the variant ligands to induce TCR aggregation and actin polymerization after stimulating A.E7 cells
for 5 min. We observed TCR polarization at points of contact with the APCs after stimulation with the WT peptide and the apoptosis-inducing Y99 and C99 peptides but
not by the A99 peptide which, by itself, does not induce
cell death. The aggregation of the TCR correlated closely
with actin polymerization detected after intracellular staining of A.E7 cells + APCs with Texas red-conjugated X
phalloidin (Fig. 4). However no distinct capping or TCR
polarization was seen after stimulation with the variants
R99 (data not shown) and A99 (Fig. 4), which fail to cause
apoptosis even though they induced readily detectable -chain
phosphorylation.
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Discussion |
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A key difference between resting and cycling mature T
cells is that antigen will stimulate lymphokine production
and proliferation in resting cells, whereas it will stimulate
lymphokine production and apoptosis in cycling T cells (8).
Death of cycling T lymphocytes occurs as part of a homeostatic feedback control mechanism and can be used to ameliorate the autoimmune demyelinating disease, experimental allergic encephalomyelitis, in mice (21, 22). For the first
time, we have examined the biological and signaling properties of TCR interaction with variant peptide-MHC class
II molecule ligands in cycling T cells. We used ligands that
differ in a key TCR contact residue in an attempt to influence the quality of TCR engagement by altering the
TCR-peptide-MHC conjugate. Cytochrome c peptide
variants at position 99, as we have used here (Y99, C99,
A99, and R99) have been shown to bind Ek with the same
affinity but to bind differently to the TCR through residues in the CDR3 (14). We have discovered that Y99 and
C99 represent a novel class of variant ligands that induce
autoregulatory apoptosis of A.E7 cells without secretion of
numerous cytokines (IL-2, IL-3, IFN-) whose production
typically accompanies TCR signaling of Th1 clones such as
A.E7. This contrasts with the WT agonist, which causes
both T cell death and cytokine production. For A.E7 cells,
we have not detected any ligands that induce activation cytokines without apoptosis. The discovery of TCR ligands
that induce death without other effector functions leads
one to consider whether other ligands could possibly elicit
cytokine secretion without death. Models suggesting a hierarchy of T cell responses do not support this possibility.
However, if distinct signaling pathways for each effector
response exist, then novel variants that stimulate activation
cytokines without apoptosis might be found (2, 3).
We observed similarities and differences in the signalling
events triggered by the WT agonist and the selectively apoptogenic variant peptides, C99 and Y99. Each of the peptides that caused apoptosis shared the ability to induce
TCR polarization in the membrane, as well as Fas-L and
TNF gene activation. At the same time, stimulation with
these ligands did not result in a consistent pattern of TCR-subunit phosphorylation, as the WT and C99 ligand induced clear-cut chain phosphorylation, but the Y99
ligand failed to do so. Moreover, the C99 and Y99 partial
agonists failed to induce other tyrosine phosphorylation
signals involving CD3-
and the ZAP-70 kinase, which
correlated with their failure to induce IL-2, IL-3, and IFN-
expression. However, despite the absence of these phosphorylation events, the C99 and Y99 peptides induce Fas-L
and TNF at levels sufficient to cause death. Thus, the TCR
signals associated with the induction of death cytokines are
potentially different from the signals necessary for inducing activation lymphokines.
Our observations concerning signal transduction are surprising in the context of standard models of WT and variant ligand signaling. Most current hypotheses are based on the lower affinity of altered ligands for the TCR and propose that the variation in TCR phosphorylation seen with such rapidly dissociating ligands arises from their short time of TCR engagement (1, 4, 5, 23, 24). In these models, only a subset of signaling events and effector functions would be induced by the variant ligands because there is rapid loss of ligand-TCR contact. If cellular responses are directly linked to the sequential set of signaling events that occur over time, one would expect a consistent pattern of signaling for ligands capable of inducing similar functional responses. We do not observe a consistent pattern of phosphorylation associated with the ability of peptides to cause apoptosis, despite the shared ability of the apoptosis-inducing ligands to cause TCR aggregation and actin polymerization. However, engagement of the TCR may elicit both positive and negative regulatory feedback loops that compete kinetically to control the extent and duration of receptor phosphorylation. As such, all ligands might initiate similar early signaling events, including immunoreceptor tyrosine-based activation motif (ITAM) phosphorylation (which is required for active polarization of the TCR), but may differ in the extent to which phosphatase recruitment has occurred at the time the cells are lysed for biochemical analysis. Alternatively, one must consider the possibility that a complex alteration in TCR signal generation arising from conformational properties of the altered ligand-TCR interaction, rather than simple kinetics, accounts for the selective induction of apoptosis.
Because mature T cell apoptosis is a powerful mechanism for establishing peripheral immune tolerance, the new class of variant ligands described here, which selectively induce apoptosis of the responding T cell, constitute a new type of potentially valuable immunomodulatory reagent. The availability of apoptosis-inducing peptides that do not elicit lymphokine secretion could permit the elimination of activated T cells without the possibility of exacerbating disease by the simultaneous release of inflammatory mediators. Likewise, selective death-inducing responses to self-ligands recognized by peripheral T cells as partial agonists, might be envisioned to play a natural role in preventing destructive autoimmune disease by eliminating self-reactive T cells without allowing them to expand or cause inflammation. Further experimentation will be needed to address these possibilities.
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Footnotes |
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Address correspondence to Dr. Michael J. Lenardo, LI, NIAID, National Institutes of Health, Building 10, Room 11N311, 10 Center Drive, MSC 1892, Bethesda, MD 20892-1892. Phone: 301-496-6754; Fax: 301-496-0222; E-mail: lenardo{at}nih.gov
Received for publication 27 November 1996 and in revised form 18 November 1997.
1 Abbreviations used in this paper: Fas-L, Fas ligand; PCC, pigeon cytochrome c; WT, wild-type.We thank Drs. J. Bolen, D. Lynch and L. Samelson for generously providing reagents. We thank Dr. I. Stefanova for helpful discussions and Drs. B. Lucas, J. O'Shea, R. Siegel and P. Schwartzberg for critically reading the manuscript.
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