Original Article |
Address correspondence to Jonathan Sprent, Dept. of Immunology, IMM4 The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: 858-784-8619; Fax: 858-784-8839; E-mail: jsprent{at}scripps.edu
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Abstract |
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Key Words: aging CD8+ cells IFN-I IL-15 T cell turnover
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Introduction |
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With regard to APCs, there are several reports that macrophages/monocytes from old donors can be inhibitory for T proliferative responses (46). Such inhibition could involve multiple mechanisms, including increased production of PGE2 (7, 8) and TGF-ß (9). Nevertheless, defects in APC function are not an invariable finding in old age (1012). Moreover, defects in T cell function are apparent in APC-free systems (2) and in situations where defined cell lines are used as APCs (3).
For T cells, studies with purified populations of APC-depleted murine T cell subsets have demonstrated that old T cells display a number of early activation defects after CD3 ligation in vitro (for a review, see reference 2). To a large extent, these changes in signal transduction are attributable to the marked increased frequency of memory-phenotype cells in old T cell populations (3, 1316). Nevertheless, at least some of the signal transduction defects found in old T cells are apparent at the level of purified naive phenotype CD4+ cells (2). The defects in these cells include decreased proliferative responses and IL-2 production and correlate with increased expression of P-glycoprotein (2, 15). These data refer to CD4+ cells. Whether aging is associated with defects at the level of CD8+ cells is less clear.
In considering the defective function of old T cells, the lifespan of these cells is an important issue. Since de novo production of T cells by the thymus declines rapidly in old age (17), most of the T cells found in old age are probably the long-term progeny of T cells generated in young life. Indeed, parking studies in mice suggest that, at a population level, T cells in this species have an almost indefinite lifespan (>2 y) (18). Since total T cell numbers remain relatively constant from puberty to old age, T cells are presumably subject to homeostatic mechanisms which balance the rate of cell death and proliferation (1921). In this respect there is a paucity of firm information on the normal in vivo physiology of T cells in aged hosts, especially with regard to T cell turnover and lifespan. Previous work from this laboratory on T cell turnover in normal young mice showed that, although both naive- and memory-phenotype cells are long lived at a population level, the background rate of proliferation (turnover) of these cells is much faster for memory- than for naive-phenotype cells (22).
For memory-phenotype (CD44hi) CD8+ cells there is increasing evidence that the survival and turnover of these cells is under the control of IL-15 (2328). In particular, numbers of CD44hi CD8+ cells are selectively reduced in IL-15/ mice (26) and the normal turnover of CD44hi CD8+ cells in vivo can be partly blocked by antibodies to IL-2Rß (CD122), an important component of the receptor for IL-15 (and IL-2; reference 27). In this paper, we show that aging is associated with a selective reduction in the turnover of CD44hi CD8+ cells and an impaired response of these cells to IL-15 in vivo (though not in vitro). The reduced turnover of CD44hi CD8+ cells in vivo was not seen after transfer to young mice. Evidence is presented that the slow turnover of CD44hi CD8+ cells in aged mice may reflect the combined inhibitory effects of Bcl-2 overexpression and increased levels of type I IFN-I.
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Materials and Methods |
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Flow Cytometry.
As described elsewhere (22, 23), surface markers on T cell subsets were defined by staining spleen and/or LN cells with Cy5-coupled anti-CD4 and anti-CD8 mAbs followed by biotin- or phycoerythrin-coupled mAbs specific for CD44, B7.1, B7.2 CD69, CD25, CD122, CD44, Ly6C, and intracellular adhesion molecule 1; these mAbs were purchased from BD PharMingen. Biotinylated mAbs were detected with Red-670 streptavidin (GIBCO BRL). T cells were stained internally for Bcl-2 and BclXL as described previously (31). In brief, cells were stained for surface markers, then fixed with paraformaldehyde, treated with saponin and then stained with a hamster antimouse Bcl-2 mAb (BD PharMingen; 3F11) or with a polyclonal rabbit antiserum specific for BclXL (provided by John Reed, Burnham Institute, La Jolla, CA) followed by FITC-conjugated antirabbit antibody. Stained cells were analyzed on a FACSortTM flow cytometer.
T Cell Turnover In Vivo.
As described previously (22), T cell proliferation in vivo was measured by maintaining mice continuously on bromodeoxyuridine (BrdU) (Sigma-Aldrich) dissolved in the drinking water. To detect BrdU incorporation, spleen and LN cells were surface stained for CD4 or CD8 and CD44 or IL-2Rß, fixed and then stained internally with an anti-BrdU mAb. For measuring BrdU incorporation on adoptive transfer, the donor and hosts differed with respect to Ly5.1 versus Ly5.2 or Thy 1.1 versus Thy 1.2. Donor and host cells were then defined by staining cells with allele-specific anti-Ly5 and anti-Thy 1 mAbs (32). In some studies, T cell turnover on adoptive transfer was measured by labeling T cells in vitro before injection with the intracellular fluorescent dye CFSE (32). As measured by flow cytometry, each division of CFSE-labeled cells causes a twofold reduction in mean fluorescence intensity (MFI) in the daughter cells, with the result that extensive cell proliferation results in a progressive step-wise reduction in MFI. For examining T proliferative responses to IL-15 and Poly I:C in vivo, graded concentrations of mouse rIL-15 (e-Bioscience) were injected intravenously and Poly I:C (100 µg/mouse) was injected intraperitoneally; in some mice, polyvalent neutralizing antiserum specific for IFN-ß (Access Biomedical) was injected intravenously at 36 and 48 h. Mice were placed on BrdU water, preceded by an intraperitoneal injection of BrdU on day 0. BrdU incorporation was measured in spleen and LNs on day 2 for IL-15 and day 3 for Poly I:C.
T Cell Proliferation In Vitro.
Purified subsets of CD44hi CD8+ cells were prepared from LNs by FACS® sorting and cultured at 0.5 x 106 cells per milliliter with graded concentrations of rIL-15 ± IFN-ß (Access Biomedical). 3[H]TdR (1 µCi/ml) was added during the last 8 h of culture. 3[H]TdR incorporation was measured on days 24.
Apoptosis.
To measure spontaneous apoptosis, nylon-wool-purified LN T cells were cultured at 106 cells per milliliter in 1 ml 24-well plates for 1 or 2 d in standard RPMI 1640 tissue culture medium supplemented with 10% FCS, glutamine, antibiotics, and 2-ME (33). Apoptosis was measured by TUNEL staining (33) after surface staining for CD4 or CD8 and CD44.
Effect of Cytokines on Bcl-2 Expression.
FACS®-sorted CD44hi CD8+ cells were cultured at 106 cells per milliliter with various cytokines (purchased from R&D Systems) in standard tissue culture medium for 18 h and then stained internally for Bcl-2 expression.
Cytokine Concentrations.
Levels of cytokines in serum and cell supernatants were measured by ELISA. In brief, serum or culture supernatants, together with known concentrations of recombinant TNF-, IFN-ß, IL-12, and IFN-
as controls, were diluted serially in saline and added to plastic plates coated with antibodies specific for IFN-
ß (polyclonal rabbit antibody purchased from Access Biomedical, San Diego, CA), IFN-
, TNF-
, or IL-12 (BD PharMingen). After incubation at 37°C for 2 h, the plates were washed, supplemented with biotinylated antibodies, washed again, followed by addition of streptavidin-peroxidase. After 1 h, the substrate O-phenyldiamine was added. The plates were analyzed with an ELISA reader after stopping the reaction with 25% H2SO4. Cytokine induction by Poly I:C (Sigma-Aldrich) was elicited in vivo by injecting mice intraperitoneally with 100 µg Poly I:C and in tissue culture by adding graded concentrations of Poly I:C to thioglycollate-induced macrophages in vitro.
RT-PCR.
Total RNA was isolated from whole spleen. First strand cDNA synthesis was performed using a Superscript Preamplification System (GIBCO BRL). PCR reactions were primed either with pairs of oligonucleotide primers corresponding to the 3' region of mouse IL-15 (23) or IFN-ß (Stratagene) or to the housekeeping gene G3PDH (23). PCR were performed for 30 cycles, with each cycle being 1 min at 94°C, 2 min at 54°C, and 3 min at 72°C. PCR samples were resolved on 1.5% agarose gels.
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Results |
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T Cell Turnover.
To examine T cell turnover, young versus old B6 mice were given BrdU continuously in the drinking water. Groups of the mice were killed at intervals and suspensions of spleen and LN cells were stained for surface markers followed by fixation and intracellular staining for BrdU using an anti-BrdU mAb. Naive- and memory-phenotype T cells were subsetted on the basis of CD44 and IL-2Rß (CD122) expression (illustrated for BrdU-labeled aged T cells in the right of Fig. 1)
. For IL-2Rß, it should be noted that expression of IL-2Rß is much higher on CD8+ than CD4+ cells (23). Hence, naive- and memory-phenotype cells were defined as being IL-2Rßint and IL-2Rßhi, respectively, for CD8+ cells and IL-2Rßlo and IL-2Rßint for CD4+ cells.
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For total unseparated CD8+ and CD4+ cells, the turnover (BrdU incorporation) of CD8+ cells in young and old mice was very similar (see Fig. 1 legend for explanation), whereas the turnover of CD4+ cells was faster in old mice than young mice.
Confirming previous findings (22), the turnover of T cell subsets in young mice was slow for naive-phenotype cells and rapid for memory-phenotype cells, both for CD8+ and CD4+ cells. The turnover of T cell subsets in old mice was distinctly different in three respects. First, in marked contrast to young mice, the difference in the turnover of naive- and memory-phenotype cells in old mice was mild or nonexistent. Second, for CD4+ cells (but not CD8+ cells) naive-phenotype cells paradoxically had a faster turnover in old mice than in young mice; a likely explanation for this finding is that, for CD4+ cells, many of the naive-phenotype cells in old mice are not truly naive but represent revertants from memory-phenotype cells (22). Third, and most importantly, the turnover of memory-phenotype CD8+ cells was much slower in old than young mice. Slow turnover of memory-phenotype cells in old mice also applied to CD4+ cells but was less prominent than for CD8+ cells.
The data in Fig. 1 A and B are representative of three different experiments on ATx mice. Data on euthymic (STx) mice were comparable and are illustrated in Fig. 1 C for CD8+ cells. For the experiments discussed hereafter, nonoperated mice were used.
T Cell Turnover on Adoptive Transfer to Young versus Old Hosts.
The key finding in the above experiments is that the turnover of memory-phenotype CD8+ cells was much slower in old mice than in young mice. A priori, this finding could reflect an intrinsic defect in old T cells per se or in the nonT microenvironment of old mice. To distinguish between these two possibilities, we examined whether the reduced turnover of old memory-phenotype CD8+ cells could be overcome by adoptively transferring old T cells to young hosts.
In the experiment shown in Fig. 2 A, purified LN T cells from old mice were transferred to Thy-1 different young hosts; 3 d later the T cell recipients plus control old and young mice were placed on BrdU water for 3 d. The notable finding was that BrdU incorporation by old CD44hi CD8+ cells transferred to young hosts was almost as high as for the population of young host CD44hi CD8+ cells (Fig. 2 A, top). These findings thus indicated that the slow turnover of CD44hi CD8+ cells in intact old mice could be substantially reversed by transferring the cells to young hosts. Similar findings applied when a mixture of Thy 1marked old and young T cells were CFSE labeled (32) and transferred to young hosts. In this situation, proliferation of donor-derived old CD44hi CD8+ cells was almost as high as for donor-derived young CD44hi CD8+ (Fig. 2 A, bottom).
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A corollary of the above data is that if a mixture of old and young T cells was transferred to young versus old hosts, the young and old T cells would both proliferate well in young hosts but poorly in old hosts. Experiments with allotype-marked CFSE-labeled cells verified this prediction (Fig. 2 C). It should be noted that in Fig. 2 B and C (but not 2 A) the host mice were pretreated with light irradiation to facilitate detection of the donor T cells. Host irradiation enhances "homeostatic" proliferation of donor T cells (32) but the results in irradiated and nonirradiated mice were qualitatively similar (compare Fig. 2 A, bottom, with Fig. 2 C, top).
Collectively, the above data indicate that, both in normal and irradiated hosts, the impaired turnover of memory-phenotype CD8+ cells in old mice reflects the particular milieu of the host environment. It should be mentioned that higher turnover of old CD8+ cells in young hosts than in old hosts has recently been reported by Ku et al. (34). Our finding that this difference applies to both irradiated and nonirradiated hosts argues against the suggestion of Ku et al. that reduced turnover in old hosts reflects increased competition for cytokines because of the increase in memory-phenotype T cells.
Surface Markers.
To examine whether the reduced turnover of old CD44hi CD8+ cells correlated with surface marker changes, old versus young T cells were stained for expression of various cell-surface molecules. Gating on CD44hi CD8+ cells showed no obvious difference in the expression of CD62L, CD45RB, CD69, CD25, B7.1, B7.2, Ly6C, and IL-2Rß on old versus young T cells (data not shown). In particular, CD44hi CD8+ cells in old mice closely resembled young T cells in expressing a high density of IL-2Rß; as discussed earlier, IL-2Rß is an important component of the receptor for IL-15 (and IL-2). The only noticeable difference for this marker was that, for CD8+ cells, the proportion of CD44hi CD8+ cells that were IL-2Rßhi was higher in old mice (9095%) than in young mice (6070%) (data not shown).
Bcl-2 and BclXL.
Since overexpression of Bcl-2 or BclXL is reported to inhibit T cell proliferation (30, 35, 36), the reduced turnover of memory-phenotype CD8+ cells in old mice could be a reflection of enhanced expression of Bcl-2 and/or BclXL. In young mice, it was shown previously that Bcl-2 expression is higher in CD44hi CD8+ cells than in CD44lo CD8+ cells but equivalent in CD44hi and CD44lo CD4+ cells (37); BclXL expression was not examined. Intracellular staining for Bcl2 and BclXL expression in young (2 mo) versus old (14 and 27 mo) T cells is shown in Fig. 3
A.
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In old mice, the notable finding was that elevation of Bcl-2 expression was much more pronounced than in young mice. Thus, Bcl-2 expression was 23-fold higher in old CD44hi CD8+ cells than in young CD44hi CD8+ cells; this finding applied both to direct MFI values for Bcl-2 staining and also to the ratio of Bcl-2:background staining (shown numerically within each middle and lower panel of Fig. 3 A). Bcl-2 expression also increased with age in CD44lo CD8+ cells, but this increase applied only to Bcl-2:background staining ratios and not to direct MFI values for Bcl-2 staining. In addition to Bcl-2 upregulation, old CD44hi CD8+ cells also showed a mild (3050%) increase in BclXL expression relative to young cells.
These findings indicated that aging was associated with a selective increase in the expression of Bcl-2, and to a lesser extent BclXL, in CD44hi CD8+ cells. By contrast, aging caused little or no change in Bcl-2 or BclXL expression in CD44hi CD4+ cells or in naive-phenotype cells.
The increased Bcl-2/BclXL expression found in old CD44hi CD8+ cells might reflect altered exposure to cytokines. To examine this possibility, purified CD44hi CD8+ cells from young mice were cultured overnight in vitro with eight different cytokines and then stained for Bcl-2 expression. Of the cytokines tested, only IL-15 caused a prominent (twofold) increase in Bcl-2 expression (Fig. 3 B, left). Two other c-controlled cytokines, IL-4 and IL-7, caused a mild increase in Bcl-2 expression, whereas IFN-I (IFN-ß), IFN-
, and TNF-
had no effect (see below). For IL-15, upregulation of Bcl-2 was restricted to CD44hi CD8+ cells and did not apply to CD44hi CD4+ cells (data not shown), which is consistent with the much lower expression of IL-2Rß on the latter cells (see above). Significantly, unmanipulated CD44hi CD8+ cells from IL-2Rß/ mice, i.e., cells that are unresponsive to IL-15 (and IL-2), showed a prominent decrease in Bcl-2 expression relative to normal cells (Fig. 3 B, right). It should be noted that, unlike IL-15/ mice, IL-2Rß/ mice contain large numbers of CD44hi CD8+ cells, presumably reflecting unrestrained MHC-driven IL-15independent responses to environmental antigens in the absence of inhibition by IL-2 (27, 29).
Apoptosis In Vitro.
Since Bcl-2 and BclXL are antiapoptotic molecules (30, 35, 36), the elevation of these molecules in old T cells would be expected to retard "background" death of these cells. To assess this possibility, purified young versus old B6 T cells were cultured in standard tissue culture medium and then TUNEL stained to assess apoptosis. The notable finding was that spontaneous apoptosis in culture was far lower with old T cells than with young T cells (Fig. 4)
. The resistance of old T cells to spontaneous apoptosis was more prominent for CD8+ cells than CD4+ cells and, especially on day 2 of culture, was more pronounced for CD44hi cells than for CD44int/lo cells. On day 2 of culture, relative to young T cells, apoptosis of old T cell subsets was reduced by 3.7-fold for CD44hi CD8+ cells, 2.4-fold for CD44int/lo CD8+ cells, 1.9-fold for CD44hi CD4+ cells, and 1.4-fold for CD44int/lo CD4+ cells. Thus, resistance of old T cells to apoptosis was most prominent in CD44hi CD8+ cells and correlated with the selective elevation of Bcl-2 in these cells. For the other T cell subsets, the significant but less prominent decrease in apoptosis of old T cells may have reflected minor upregulation of Bcl-2, BclXL, and/or other Bcl-2 family members (which were not studied).
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Cytokine Levels.
The capacity of IL-15 to upregulate Bcl-2 expression on CD44hi CD8+ cells raised the possibility that the overexpression of Bcl-2 in aged CD44hi CD8+ cells reflected increased levels of IL-15 in the aged microenvironment. Because IL-15 is difficult to detect at the protein level, we measured IL-15 at the level of mRNA. Since a spectrum of different cell types, including stromal cells, synthesize IL-15 (25, 28), IL-15 mRNA was measured in whole spleen rather than in cell suspensions. As shown in Table I, relative to G3PDH as a "housekeeping" control, IL-15 mRNA levels in spleens of young and old mice were quite similar.
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In addition to stimulating IL-15 production, IFN-I is known to exert an antiproliferative effect on T cells (3941). Consistent with this observation we have found that, in young mice, bystander proliferation of CD44hi CD8+ cells induced by injection of Poly I:C falls to low levels when the dose of Poly I:C is raised above a certain level (data not shown). It is conceivable therefore that the reduced turnover of CD44hi CD8+ cells in old mice is due in part to a direct antiproliferative effect on T cells mediated by the increased levels of IFN-I. If so, it would follow that old mice would show decreased responsiveness to exogenous IL-15 and IL-15-inducing agents such as Poly I:C (which induces IL-15 synthesis via production of IFN-I). In line with this prediction, "bystander" proliferation of CD44hi (IL-2Rßhi) CD8+ cells elicited by injection of IL-15 (Fig. 6 A) and Poly I:C (Fig. 6 B) was substantially lower in old than young mice. Significantly, for Poly I:C, this reduced responsiveness in old mice could be largely reversed by injection of antiIFN-I antibody (Fig. 6 B).
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Discussion |
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Bcl-2 Overexpression.
For young mice, the data confirm the recent report (37) that Bcl-2 expression is higher in memory-phenotype CD8+ cells than in other T cell subsets. In extending this finding, we observed that Bcl-2 expression in CD44hi CD8+ cells was even higher in old mice but was not elevated in CD44hi CD4+ cells. We also found that, like Bcl-2, BclXL expression was elevated in CD44hi cells, both in young and old mice. Unlike Bcl-2, however, BclXL expression was upregulated in both CD44hi CD8+ and CD44hi CD4+ cells and, for CD44hi CD8+ cells, was only slightly increased with aging. In agreement with the known anti-apoptotic function of Bcl-2 (35), selective overexpression of Bcl-2 in old CD44hi CD8+ cells was associated with a prominent decrease in spontaneous apoptosis of these cells in vitro.
Why Bcl-2 expression is selectively upregulated in CD44hi CD8+ cells and is enhanced by aging is unclear. Based on the effects of adding cytokines to purified T cells in vitro, Bcl-2 upregulation may reflect contact with cytokines, especially IL-15. Consistent with this finding, Bcl-2 expression was markedly reduced in IL-2Rß/ CD44hi CD8+ cells, i.e., in T cells that lack a key receptor for IL-15 (and IL-2). Hence continuous contact with IL-15 in vivo may explain why Bcl-2 expression is much higher in CD44hi CD8+ cells (which are IL-2Rßhi and therefore IL-15 responsive) than in CD44hi CD4+ cells (IL-2Rßint). However, it is more difficult to account for the further elevation in Bcl-2 expression found in old CD44hi CD8+ cells. Here, the simplest idea is that IL-15 levels are increased in old age. However, at least at the level of mRNA in whole spleen, this was not the case, IL-15 levels being only fractionally higher in old spleen than young spleen. Nevertheless, the possibility that local concentrations of IL-15 in the T cell areas, e.g., on dendritic cells (42), are higher in old mice cannot be discounted. An alternative possibility is that enhanced Bcl-2 expression in old CD44hi CD8+ cells reflects enhanced contact with other cytokines, e.g., IL-4 or IL-7. Thus, like IL-15, these two cytokines enhanced Bcl-2 expression in young T cells in vitro. As yet, however, we have not measured levels of IL-4 and IL-7 in old versus young mice.
Whatever the mechanisms controlling Bcl-2 expression, it is notable that overexpression of Bcl-2 in old CD44hi CD8+ cells correlated with a selective decrease in the background turnover of these cells in vivo. Hence, in view of the evidence that Bcl-2 overexpression impairs entry into cell cycle (30, 35, 36), the enhanced expression of Bcl-2 in old CD44hi CD8+ cells could be at least partly responsible for the slow turnover of these cells in vivo. Support for this idea is provided by the finding that, in young mice, the turnover of CD44hi CD8+ cells was much slower for Bcl-2 transgenic T cells than for normal T cells. For CD44hi CD4+ cells, aging was associated with little change in either Bcl-2 or BclXL expression and only a mild decrease in T cell turnover (see below). Interestingly, others have reported that, relative to young mice, CD44hi CD4+ cells from older lupus-prone BXSB mice show reduced T cell turnover and apoptosis and increased BclXL (but not Bcl-2) expression (43). Hence, as for Bcl-2 in CD8+ cells, enhanced BclXL in CD4+ cells may have an antiproliferative effect on T cell turnover. It should be mentioned that slow turnover of lymphoid cells in old mice has also been reported for B cells (44). Whether this finding is related to Bcl-2/BclXL expression or increased IFN-I levels (see below) has yet to be studied.
Inhibition by IFN-I.
Of the limited number of cytokines studied, levels of three cytokines, IFN-I, IFN-, and IL-12 were about threefold higher in the serum of old mice than in young mice. Elevation of IFN-I (which was also found at the mRNA level) is of particular interest because this cytokine can have two diametrically opposite effects on CD44hi CD8+ cells, i.e., (i) increased proliferation induced by stimulation of IL-15 synthesis (23) and (ii) inhibition of proliferation as the result of a direct inhibitory influence on T cells (3941). Since IL-15 levels were not noticeably enhanced in old mice, we considered the possibility that the reduced turnover of old CD44hi CD8+ cells in vivo reflected a direct antiproliferative response mediated by the increased concentration of IFN-I found in old mice. In accordance with this idea, in vivo proliferation of CD44hi CD8+ cells after injection of IL-15 and IL-15-inducing agents (Poly I:C) was clearly lower in old mice than in young mice. Significantly, for Poly I:C, the reduction in T cell turnover was reversed by injection of antiIFN-I antibody. Likewise, preliminary work has shown that (a) antiIFN-I antibody injection partly reverses the slow tempo of CD44hi CD8+ cell turnover in old mice and (b) the slow turnover of these cells is much less apparent in old IFN-IR/ mice (unpublished data).
If the reduced response of old T cells to IL-15 in vivo reflected inhibition by IFN-I, it would follow that old CD44hi CD8+ cells would respond well to IL-15 in vitro in the absence of IFN-I but would be highly sensitive to inhibition by addition of exogenous IFN-I. This was indeed the case. In fact, concentrations of IFN-I as low as 40 U/ml caused an appreciable reduction in the in vitro response to IL-15; this concentration of IFN-I was close to the level found in the serum of old mice. It is important to note that in vitro responses to IL-15 were highly sensitive to even moderate (twofold) alterations in the concentration of IFN-I in vitro. This finding supports the view that the approximately threefold increase in IFN-I levels found in old mice is sufficient to exert an antiproliferative effect on T cells.
Since the antiproliferative effect of IFN-I is not restricted to CD44hi CD8+ cells, enhanced exposure to IFN-I in old mice would be expected to retard the turnover of CD44hi CD4+ cells as well as CD44hi CD8+ cells. On this point it should be noted that the decrease in T cell turnover in old mice did affect CD44hi CD4+ cells, though to a lesser extent than CD44hi CD8+ cells. Nevertheless, the fact that slow turnover of old T cells was more prominent in CD44hi CD8+ cells than CD44hi CD4+ cells implies that inhibition by IFN-I is not the sole explanation. For this reason, we favor the idea that the selective decrease in the turnover of CD44hi CD8+ cells in old mice reflects the combined inhibitory effects of two different mechanisms working together, i.e., enhanced IFN-I levels plus Bcl-2 overexpression.
The significance of reduced T cell turnover in aging is a matter for speculation. Since thymic function is poor after middle age, maintaining T cell numbers in old age presumably hinges on continuous contact with factors that promote long-term T cell survival. For CD44hi CD8+ cells, at least in young mice exposure to IL-15 is crucial for survival. How IL-15 protects CD44hi CD8+ cells is unclear but, based on the present data, upregulation of Bcl-2 and/or other antiapoptotic molecules is a likely possibility. Placing the survival of T cells under the control of life-sustaining cytokines, however, has the potential danger that even transient increases in these growth factors could cause T cell numbers to rise to excessive levels. Hence to maintain normal homeostasis, one can envisage a delicate equilibrium where factors controlling T cell survival/proliferation are balanced by other factors that either promote cell death or inhibit proliferation. Hence, the increase in Bcl-2 expression and IFN-I levels in aging could be a manifestation of a general need to regulate T cell homeostasis in senescence. It is worth noting, however, that Bcl-2 and IFN-I do not act solely as negative regulators. Thus, countering their inhibitory effect on proliferation, Bcl-2 (30) and IFN-I (45) may augment cell survival via their antiapoptotic properties (and via IL-15 production by IFN-I). Hence, the control of T cell homeostasis in aging is undoubtedly highly complex and presumably reflects the interplay of many different factors.
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Acknowledgments |
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This work was supported by grants CA38355, CA25803, AI21487, AI32068, AI45809, AI41079, and AG01743 from the United States Public Health Service. C.D. Surh is a Scholar of The Leukemia & Lymphoma Society. Publication no. 13459-IMM from the Scripps Research Institute.
Submitted: July 24, 2001
Revised: December 6, 2001
Accepted: December 11, 2001
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Footnotes |
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References |
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