By
From the * Department of Immunology, the Department of Medical Biophysics, and Amgen Institute,
University of Toronto, Toronto, Ontario M5S 1A8, Canada; and the § Howard Hughes Medical
Institute and
The Rockefeller University, New York 10021
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Abstract |
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4-1BB ligand (4-1BBL) is a member of the tumor necrosis factor (TNF) family expressed on
activated antigen-presenting cells. Its receptor, 4-1BB, is a member of the TNF receptor family
expressed on activated CD4 and CD8 T cells. We have produced a soluble form of 4-1BBL using the baculovirus expression system. When coimmobilized on plastic with anti-CD3, soluble
4-1BBL induces interleukin (IL)-2 production by resting CD28+ or CD28 T cells, indicating
that 4-1BBL can function independently of other cell surface molecules, including CD28, in
costimulation of resting T cell activation. At low concentrations of anti-CD3, 4-1BBL is inferior to anti-CD28 in T cell activation. However, when 4-1BB ligand is provided together with
strong TCR signals, then 4-1BBL and anti-CD28 are equally potent in stimulation of IL-2
production by resting T cells. We find that TNF receptor-associated factor (TRAF)1 or
TRAF2 associate with a glutathione S-transferase-4-1BB cytoplasmic domain fusion protein in
vitro. In T cells, we find that association of TRAF1 and TRAF2 with 4-1BB requires 4-1BB cross-linking. In support of a functional role for TRAF2 in 4-1BB signaling, we find that resting T
cells isolated from TRAF2-deficient mice or from mice expressing a dominant negative form
of TRAF2 fail to augment IL-2 production in response to soluble 4-1BBL. Thus 4-1BB, via
the TRAF2 molecule, can provide CD28-independent costimulatory signals to resting T cells.
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Introduction |
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Tcells require two signals for activation, an antigen-specific MHC-restricted signal through the TCR and a
second costimulatory signal. CD28 on T cells is widely considered as the primary receptor for delivering costimulatory
signals to resting T cells (1). However, CD28 T cells are
not defective in all responses, suggesting the existence of other
costimulatory pathways (2). One such alternate costimulatory pathway involves the TNFR family member 4-1BB
(CDw137), a T cell activation antigen found on activated
CD4 and CD8 T cells (5, 6). The ligand for 4-1BB is
found on activated B cells, macrophages, and cultured dendritic cells, as well as on several B lymphomas and the thymoma, EL4 (7).
A number of studies have shown a role for 4-1BB in T cell activation using either transfected ligand (7), antibodies against the 4-1BB molecule (6, 12), or blocking studies with a soluble form of the 4-1BB receptor (10, 11, 15, 16). Anti-4-1BB antibodies are effective in the activation of T cells that have been preactivated via their TCR to induce high levels of 4-1BB receptor expression (12), but are poorer agonists for induction of IL-2 by resting T cells (6). More recently, Shuford et al. have shown that anti-4-1BB antibodies induce higher levels of proliferation of CD8 T cells compared with CD4 T cells, leading to the suggestion that 4-1BB is primarily a costimulatory molecule for CD8 T cells (14). In contrast, studies with APCs that express 4-1BB ligand (4-1BBL)1 have shown that 4-1BB interaction with its ligand can participate in induction of proliferation and IL-2 and IL-4 production by CD4 T cells. This role for 4-1BBL in the development of Th1 and Th2 cells is most apparent in the absence of a strong B7-CD28 interaction (11, 16).
Despite the accumulating body of evidence on the importance of 4-1BB as a costimulatory receptor on activated
CD4 and CD8 T cells, there is controversy as to whether
4-1BBL alone can provide a costimulatory signal for resting
T cells independently of other molecules present on the
APC. In this report we describe the production of a soluble
form of 4-1BBL (s4-1BBL) using the baculovirus expression system. When coimmobilized on plastic with anti-CD3 or when cross-linked in solution through its influenza
hemagglutinin (HA) epitope tag, we find that s4-1BBL is a
potent activator of IL-2 production by isolated high density
CD28+ or CD28 T cells. When TCR signals are limiting,
we find that immobilized anti-CD28 is more effective than
4-1BBL in inducing IL-2 production. However, at higher
anti-CD3 concentrations, 4-1BBL and anti-CD28 are equally
potent in inducing IL-2 production by resting T cells. Thus, isolated 4-1BBL can costimulate resting T cells via a
CD28-independent pathway.
The observation that 4-1BB signaling can replace CD28
signaling in costimulation of T cell activation under some
circumstances raises the question of how signals from the
4-1BB receptor synergize with signals from the TCR to
induce high level IL-2 production. 4-1BB is a member of
the TNFR superfamily. Other members of this family have
been shown to signal via the TNFR-associated factor (TRAF) family of signaling molecules first identified by
their ability to interact with the cytoplasmic domains of
TNFR family members (17, 18). Six members of the
TRAF family (TRAF1-6) have been identified to date
(17). The TRAF proteins appear to function as adapter
proteins that link TNFR family members to downstream
signaling pathways. TRAF family members have in common a conserved TRAF-C domain involved in homotypic
interactions or in heterotypic interactions with other TRAF
molecules and with the cytoplasmic tails of TNFR family
members. The TRAF2 molecule has been shown to interact directly with the cytoplasmic tails of CD40, CD30, and
TNFR2 and indirectly with TNFR1 (17, 18, 26) and has
been shown to induce both nuclear factor (NF)-B and c-Jun
NH2-terminal kinase (JNK) activation in response to TNF
stimulation (26, 32).
Transgenic mice expressing a dominant negative (DN)
form of TRAF2 (TRAF2 241-501) in their lymphoid cells
(TRAF2 DN mice) have reduced JNK activity, but relatively normal NF-B activation (35). Similar results have
also been obtained using mice that have been rendered
TRAF2 negative by gene targeting (36). Here we show
that T lymphocytes isolated from traf2
/
or TRAF2 DN
mice fail to augment IL-2 production in response to s4-1BBL. Thus the 4-1BB costimulatory signal leading to IL-2 production is dependent on the TRAF2 molecule.
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Materials and Methods |
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Cell Lines, Mice, Antibodies and Reagents.
The T hybrid, C8.A3, was obtained from Dr. Laurie Glimcher (Harvard Medical School, Boston, MA). CD28Generation of s4-1BBL.
4-1BBL is a type II glycoprotein. To generate s4-1BBL in insect cells, we linked cDNA encoding the 4-1BBL extracellular COOH-terminal domain to the signal sequence from myelin-associated glycoprotein, a signal sequence previously shown to be effective in directing the secretion of heterologous proteins in the baculovirus expression system (reference 42 and see Fig. 1 A). An HA epitope tag was linked to the COOH terminus of the 4-1BBL molecule to facilitate isolation of the secreted molecule.
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Purification of s4-1BBL.
s4-1BBL was produced and purified as follows. A 3-liter suspension culture of 1-2 × 106 SF9 cells/ml grown in SF900II (GIBCO BRL) at room temperature was infected with recombinant virus at a multiplicity of infection of 10- 20 and harvested 6 d after infection. s4-1BBL molecules were purified from the supernatant by affinity chromatography on 12CA5 Sepharose. 12CA5 Sepharose 4B was prepared using cyanogen bromide-activated Sepharose (Pharmacia Biotech) according to the manufacturer's instructions. To ensure that effects of s4-1BBL were not influenced by the presence of endotoxin, the level of endotoxin in the purified s4-1BBL was measured using the Limulus amebocytes lysate QCL 1000 kit (BioWhittaker Inc., Walkersville, MD) and found to be less than 0.1 U/ml.Generation of 4-1BB Cytoplasmic Tail (CT)-GST Fusion and Measurement of 4-1BB CT-TRAF Association.
The entire cytoplasmic tail of 4-1BB was obtained by PCR from the 4-1BB full length cDNA using primers 5'-GGGAATTCAAATGGATCAGGAAAAAATTCCC-3' and 5'-GGGGATCCTCACAGCTCATAGCCTCCTCCTC-3'. Amplified PCR fragments were sequenced and fused in-frame to GST by cloning into EcoRI and BamHI sites of pEBG, a mammalian GST expression vector, to generate pEBG-4-1BBCT. The plasmids, pEBG, pEBG-4-1BB, and pEBG-CD30CT (28), were transfected into 293 cells. 48 h after transfection, GST and GST fusion proteins were purified with glutathione beads. For in vitro association experiments, the full-length murine TRAF1 and TRAF2 cDNA in pBluescript (Stratagene Inc., La Jolla, CA) were transcribed and translated in vitro using the TNT coupled reticulocytes system (Promega) with 35S-labeled methionine. Equal amounts of in vitro translated TRAF1 or TRAF2 were incubated in binding buffer (PBS containing 0.1% NP-40, 0.5 mM DTT, 10% glycerol, 1 mM PMSF, and 2 µg/ml aprotinin) with ~5 µg of fusion protein bound to glutathione beads for 45 min at 4°C. After washing five times with binding buffer, the proteins were eluted by boiling in SDS sample buffer for 5 min and subsequently analyzed by SDS-PAGE.Measurement of TRAF1 and TRAF2 Association with 4-1BB in C8.A3 T Cells.
For measurement of TRAF1 and TRAF2 association in C8.A3 T hybrids, 2 × 107 C8.A3 cells were incubated on ice with 5 µg/ml of anti-4-1BB (1AH2) or with rat anti- ICAM-1 (YN1) control antibody for 5 min. The antibody was cross-linked with 20 µg/ml of RG7 (anti-ratT Cell Isolation.
APCs were depleted from spleen or lymph node cell suspensions in HBSS (GIBCO BRL)/2.5% FCS/50 µM 2-ME, with a cocktail of antibodies, anti-class II (Y3P), anti-B220, anti-heat stable antigen (M1/69), anti-MAC-1, and anti-CD11c (N418) each at a final concentration of 10 µg/ml at 4°C for 30 min. A 1:10 dilution of baby rabbit complement (Cedarlane Labs. Ltd., Hornby, Ontario, Canada) was added, and the cultures were further incubated at 37°C for 40 min. To remove adherent cells, the cell suspensions were passaged through a Sephadex G10/nylon wool column, and then centrifuged through Percoll gradients consisting of 60, 70, and 80% Percoll layers. Small (high density) T cells were isolated from the 70/80% interface and used in all subsequent experiments.T Cell Activation Assays.
Monoclonal anti-CD3 (145-2C11) was immobilized on the surface of 96-well plates (Nunc, Gaithersburg, MD) by incubating for 5 h at 37°C. Where indicated, s4-1BBL or anti-CD28 antibodies were added to the wells at the same time. 0.5-1 × 105 small high density T cells, from either C57BL/6, CD28 ![]() |
Results |
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At the time we began these studies, Hurtado et al. had shown that antibodies to 4-1BB could activate preactivated T cells but were rather poor in the activation of resting T cells (12). In contrast, we had found that B lymphomas expressing 4-1BBL could induce high levels of IL-2 and IL-4 production by resting T cells (10, 16). Therefore, it was possible either that 4-1BBL was more potent in activating resting T cells than were available antibodies or that other molecules on the APC contributed to T cell activation by 4-1BBL. To distinguish these possibilities, we generated a soluble form of 4-1BBL using the baculovirus expression system.The entire extracellular domain of 4-1BBL was expressed in isolation with the addition of an influenza HA epitope tag at the COOH terminus of the protein to facilitate purification (Fig. 1 A). Fig. 1 B shows a Coomassie blue-stained gel of purified s4-1BBL isolated from the insect cells using 12CA5 (anti-HA) affinity chromatography. It can be seen that s4-1BBL forms a disulfide-linked dimer of ~63 kD, consistent with the expected molecular weight for the glycosylated form of the extracellular domain. The band is slightly heterogeneous, possibly due to variable glycosylation or due to limited proteolysis.
Activation of CD28+ and CD28To assess the activity of s4-1BBL in the activation of resting
T cells, high density resting T cells from the spleens of
CD28+ or CD28 mice were isolated by complement and
Sephadex G10 depletion of APCs followed by Percoll gradient fractionation as described in Materials and Methods.
CD28
T cells were used to eliminate the possibility of any
contribution from CD28 signaling. Fig. 2 A shows IL-2
production by purified T cells responding to immobilized
anti-CD3 in the presence or absence of immobilized s4-1BBL, added at 1 µg/ml. It can be seen that for both
CD28+ and CD28
T cells, no IL-2 is generated in response to immobilized anti-CD3 alone, but that coimmobilization of s4-1BBL leads to significant IL-2 production.
s4-1BBL added in solution did not stimulate a response,
but s4-1BBL added in solution together with anti-HA antibody to cross-link the molecule via the HA tag was also effective in costimulation (data not shown). Fig. 2 B shows
the proliferation of CD28
T cells in response to immobilized anti-CD3 alone or with 1 or 10 µg/ml immobilized
s4-1BBL. It can be seen that at suboptimal anti-CD3 concentrations, s4-1BBL enhances T cell proliferation. Fig. 2
C shows that the effect of s4-1BBL can be fully blocked by
coculture with a soluble form of the receptor, 4-1BB-AP,
whereas AP alone had no such effect. Thus, the enhancement of IL-2 production in response to 4-1BBL is specific
to the soluble ligand.
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Fig. 3 compares the sensitivity of CD28+ and CD28 T
cells to s4-1BBL. It can be seen that the IL-2 response of
CD28+ and CD28
T cells saturates at a concentration of
s4-1BBL of between 2 and 5 µg/ml. This titration experiment was repeated using a different batch of purified s4-1BBL and showed a similar saturation point (data not
shown). The response of CD28
T cells to s4-1BBL is
somewhat reduced compared with that of CD28+ T cells.
Higher responses of CD28+ versus CD28
T cells to s4-1BBL might be due to low level expression of B7.2 on the
T cells or due to the presence of B7 on contaminating
APCs. Differences between the magnitude of the response
of CD28+ and CD28
T cells to s4-1BBL were not apparent in all experiments (for example, see Fig. 2) but tended
to vary with the particular T cell preparation. Similar differences between CD28+ and CD28
T cell responses to
4-1BBL were previously observed using 4-1BBL on APCs
(11). Flow cytometry analysis of CD28+ and CD28
T
cells after overnight treatment with anti-CD3 showed similar levels of induction of 4-1BB on the surface (data not
shown). Thus the difference in response to s4-1BBL on
CD28+ or CD28
T cells does not appear to be due to differential expression of 4-1BB in response to anti-CD3
treatment of CD28+ or CD28
T cells.
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4-1BB is an activation antigen on T cells. 4-1BB
mRNA appears within 3 h of anti-CD3 stimulation (45),
but surface expression of 4-1BB is not apparent until several hours later (6). Therefore, it was important to establish
the kinetics of response to s4-1BBL. Fig. 4 shows IL-2 production by resting CD28 T cells at various times after exposure to immobilized anti-CD3 plus immobilized s4-1BBL. It can be seen that IL-2 production by resting T
cells responding to anti-CD3 plus immobilized s4-1BBL is
detectable by 24 h with further increases in IL-2 production by 48 h. The kinetics of response to s4-1BBL is similar
to the response of T cells to 4-1BBL expressed on B lymphomas, where we detected some IL-2 production by 12 h
with increasing amounts at 24-48 h (11). Thus the kinetics
of the response to s4-1BBL appears to be rapid enough to
explain the ability of 4-1BBL to activate resting T cells and
suggests that quite low levels of 4-1BB on the T cell surface
may be sufficient to provide T cell costimulation.
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To compare the efficacy of the 4-1BB
versus CD28 costimulatory pathways for resting T cell activation, we compared T cell responses to immobilized anti-CD28 and immobilized s4-1BBL in the presence of various
doses of anti-CD3 as shown in Fig. 5. Anti-CD28 was titrated in a separate experiment (data not shown) and the
concentration which gives maximum stimulation when
immobilized with 1 µg/ml of anti-CD3 (10 µg/ml anti-CD28) was compared with s4-1BBL at 5 µg/ml. It can be
seen that at low doses of anti-CD3, anti-CD28 is more effective than s4-1BBL in stimulating IL-2 production by
resting CD28+ T cells. However, if one increases signals
through the TCR, via increasing the density of immobilized anti-CD3, then 4-1BBL and anti-CD28 induce similarly high levels of IL-2 production by resting CD28+ T
cells. As expected, only s4-1BBL but not anti-CD28 enhanced IL-2 production by CD28 T cells. Thus, 4-1BBL
can provide a similar level of costimulation as anti-CD28
when provided with a strong TCR stimulus, but is less effective when signals through the TCR are limiting.
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The mechanism by which 4-1BB signals T cells to costimulate IL-2
production is not known. 4-1BB is a member of the
TNFR family of signaling molecules. As discussed above,
the TRAF family of signaling molecules appear to function
as adapter proteins in the signal transduction cascades leading to NF-B and JNK activation by members of this family. Since TRAF2 has been shown to interact directly with
the cytoplasmic tails of several TNFR family molecules, we
chose to focus on a possible role for TRAF2 in 4-1BB signaling. Therefore, the 4-1BB cytoplasmic domain was expressed as a GST fusion protein in 293 cells. Purified 4-1BB-
CT-GST bound to glutathione Sepharose was used to probe
the association of the 4-1BB cytoplasmic domain with in
vitro translated 35S-methionine labeled TRAF1 and TRAF2
proteins (Fig. 6). TRAF1 and TRAF2 were found to associate with 4-1BB-CT-GST as well as CD30-GST (included as a positive control). No association was observed
between TRAF1 and TRAF2 and GST alone.
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We also attempted to detect TRAF association with the 4-1BB-CT-GST protein in 293 cells, but were not successful (data not shown), which suggests that in cells, aggregation of 4-1BB might be required to induce TRAF association as has been shown for CD40/TRAF association (44). To test this hypothesis, we analyzed the association of TRAF1 and TRAF2 with full-length 4-1BB in the T cell hybridoma C8.A3. This T cell hybridoma has been shown previously to respond to 4-1BB signaling and expresses low levels of 4-1BB in the resting state, with further upregulation of 4-1BB after overnight treatment with anti-CD3 (10). C8.A3 cells were incubated with anti-4-1BB and goat anti-rat Ig for 15 min at 37°C or on ice, after which time the cells were lysed and protein G-Sepharose added to immunoprecipitate the complex. When 4-1BB was cross-linked at 0°C little or no TRAF1 or TRAF2 could be detected by Western blot analysis of the immunoprecipitated material (Fig. 7). In contrast, aggregation of 4-1BB at 37°C using anti-4-1BB followed by anti-rat Ig induced the association of TRAF1 and TRAF2 with the 4-1BB precipitates. Cross-linking of ICAM-1 on the T cells using rat anti-ICAM-1 and anti-rat Ig at 37°C did not result in significant levels of TRAF1 or TRAF2 in the ICAM-1 immunoprecipitates (Fig. 7). Thus, the presence of TRAF1 and TRAF2 in the immunoprecipitates is specific to the 4-1BB molecule. These results show that cross-linking of 4-1BB on T cells results in its enhanced association with TRAF1 and TRAF2 and that there is little or no TRAF1 and TRAF2 associated with 4-1BB in the resting state.
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Based on the association of the 4-1BB cytoplasmic domain with TRAF2 (Figs.
6 and 7) and the importance of TRAF2 in JNK activation in response to TNF, we explored the functional role of
TRAF2 using T cells isolated from TRAF2 mice, as well
as using T cells isolated from TRAF2 DN mice.
To test the effect of the TRAF2 DN mutant on 4-1BB signaling leading to IL-2 production, we isolated high density resting T cells from TRAF2 DN mice and their transgene negative littermates. Fig. 8 compares IL-2 production by the T cells in response to anti-CD3 alone, anti-CD3 plus immobilized s4-1BBL or anti-CD3 plus immobilized anti-CD28 antibody. For the wild-type T cells, immobilized s4-1BBL clearly augments the response of resting spleen or lymph node T cells to immobilized anti-CD3. As was observed in Fig. 5, the effects of s4-1BBL on IL-2 production are smaller than those of anti-CD28. The TRAF2 DN T cells showed a higher background response to anti-CD3 alone and addition of s4-1BBL actually led to a decrease in response of the TRAF2 DN T cells. In contrast, anti-CD28 augmented IL-2 production in response to anti-CD3 in both the wild-type mice and TRAF2 DN mice. Although responses to anti-CD28 were retained in all instances, with lymph node T cells we sometimes observed a lower response to anti-CD28 in transgenic T cells compared with the nontransgenic controls (e.g., anti-CD28 at 10 µg/ml in Fig. 8). In contrast, splenic T cells sometimes showed hyperresponsiveness to anti-CD28 (e.g., anti-CD28 2 µg/ml, Fig. 8). In spite of the variable response to anti-CD28, the observation that the TRAF2 DN mice largely retain responses to anti-CD28 but fail to show augmentation of IL-2 production in response to immobilized s4-1BBL suggests that the TRAF2 signaling molecule is required for 4-1BB signaling.
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Fig. 9 shows a similar experiment carried out using T
cells isolated from the lymph nodes of TRAF2 mice. As
described elsewhere, TRAF2
mice have small lymphoid
organs and die on average at day 10-14 after birth (36).
However, some mice survived to 3 wk and were used for
these experiments. The spleens of these mice are atrophied and could not be used for T cell isolation, but a small number of resting T cells could be isolated from the lymph
nodes of these animals. It can be seen that the T cells from
the TRAF2
mice retain the ability to respond to anti-CD28, but show no enhancement of anti-CD3 signaling
by immobilized 4-1BBL. Again, the response to anti-CD3
alone was higher in the TRAF2-deficient animals than the
littermate controls and the addition of 4-1BBL actually reduces the response to anti-CD3 alone. Similarly to the results with lymph node T cells from the TRAF2 DN mice
(Fig. 8), the response of lymph node T cells to anti-CD3
plus anti-CD28 was also somewhat reduced.
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In addition to TRAF2, the 4-1BB cytoplasmic domain also interacts with TRAF1 (Figs. 6 and 7). T cells from transgenic mice that overexpress TRAF1 have been shown to proliferate normally in response to TCR engagement, but are resistant to antigen-induced apoptosis (38). Consistent with the observations that TRAF1 overexpression does not influence T cell proliferative responses (38), we find that T cells from mice overexpressing TRAF1 showed no differences in IL-2 production in response to anti-CD3 plus immobilized s4-1BBL compared with wild-type controls (data not shown).
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Discussion |
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The delivery of a signal through the CD28 molecule has
been shown to be critical for activation of T cells and for
prevention of induction of cell death or an anergic state.
For a costimulatory receptor to prevent the induction of
anergy, it is thought that the costimulatory signal must be
delivered simultaneously or within a few hours of signaling
through the TCR (46). Previous studies using antibodies to
4-1BB to stimulate T cells have found that unprimed T
cells require 4-5 d to respond to anti-4-1BB, but that T
cells that have been preactivated for 2-3 d to induce a high level 4-1BB expression, respond within 48 h (6, 12). Our data using 4-1BBL on APCs (11) or using s4-1BBL (this
report) show that T cells can produce IL-2 in response to
4-1BBL within 24 h in the absence of CD28 costimulation. Other studies in which antibodies to 4-1BB have
been used to costimulate with anti-CD3 either used preactivated T cells (12) or did not separate resting from activated T cells (14). Because B7 family members are expressed on activated T cells, one cannot rule out that low
levels of B7 were also contributing to the response in these
studies (12, 14). Although we have previously shown that
CD28 T cells can respond to 4-1BBL expressed on APCs
(16), in those studies it was possible that other molecules
present on the cell surface contributed to this signal. Our
study therefore used purified resting T cells from CD28
mice and soluble 4-1BBL to test the role of 4-1BBL in isolation. The data presented here clearly show that 4-1BBL
alone can provide an effective costimulatory signal for resting T cell activation, independent of other cell surface molecules including the CD28 molecule.
The ability of s4-1BBL to induce IL-2 in T cells more rapidly than anti-4-1BB antibody may be due to the natural ligand for 4-1BB providing a more potent signal than that delivered by antibody ligation. Although surface expression of 4-1BB is not obvious until at least 24 h after anti-CD3 treatment of resting T cells (6), 4-1BB mRNA is readily detectable within 3 h of anti-CD3 treatment (45). Our data showing that functional responses to isolated 4-1BBL occur within 24 h of T cell activation suggests that it may take only a few 4-1BB molecules on the T cell surface to allow costimulatory signals to be transduced. Thus, our data show that 4-1BBL can, under appropriate conditions, function in a similar way to and independently of the CD28 molecule to provide a costimulatory signal for induction of IL-2 production by resting T cells.
A comparison of the costimulatory activity of immobilized anti-CD28 and immobilized s4-1BBL (Fig. 5) showed that anti-CD28 is more effective than immobilized s4-1BBL in inducing IL-2 production at low doses of anti-CD3. However, at higher concentrations of anti-CD3, one can achieve similar levels of IL-2 production in response to CD28 or 4-1BB engagement. CD28 is expressed on resting T cells, whereas 4-1BB is expressed only after signaling through the TCR. When anti-CD3 is bound to the plates at low density, the level of 4-1BB receptor induced may be limiting, thus explaining the poorer response to s4-1BBL at low anti-CD3 concentrations. Alternatively or additionally, the lower response to s4-1BBL at low anti-CD3 doses might be due to 4-1BBL providing a weaker costimulatory signal than anti-CD28. This weaker signal from 4-1BBL- 4-1BB interaction may be compensated for by increasing signals through the TCR. Nevertheless, when signals through the TCR are optimized, 4-1BBL can function as well as anti-CD28 in induction of IL-2 production by resting T cells (Fig. 5).
Shahinian et al. have shown that CD28 T cells are unimpaired in their immune response to LCMV infection (2).
Interestingly, there is evidence that it is the duration of
TCR signaling that allows CD28-independent immune responses to LCMV (47). In light of our data, one could interpret the data of Kündig et al. (47) as suggesting that antigens that induce strong signals through the TCR may
allow other CD28-independent costimulatory pathways, such as the 4-1BB pathway, to take over. The kinetics of
the response to s4-1BBL (Fig. 4) suggest that if signal 1 is
strong enough, then 4-1BB can be induced and receive
sufficient signals to induce IL-2 production within 24 h.
A critical question is how the various cell surface molecules involved in T cell activation integrate the signals from distinct signal transduction pathways to induce high level IL-2 gene expression. T cell receptor signaling and CD28 signaling have been shown to synergize at the level of JNK (48). TRAF2 has been implicated in the ability of both TNFR1 and CD40 to activate JNK (32). Therefore, an attractive hypothesis is that 4-1BB signaling can replace CD28 signaling by synergizing with the TCR at the level of JNK activation. As a first step toward testing this model, we have analyzed the role of TRAF2 in T cell activation by 4-1BB.
Using a GST fusion protein, we showed that the cytoplasmic domain of 4-1BB could associate with TRAF1 and TRAF2 in vitro (Fig. 6). However, attempts to demonstrate an association between the 4-1BB CT and TRAF1 and 2 in 293 cells using the same GST fusion protein were not successful. This suggested that aggregation of 4-1BB might be required for TRAF association in cells. In support of this, we found that TRAF1 and TRAF2 association with 4-1BB in a T cell hybrid required cross-linking of 4-1BB at 37°C. During the revision of this manuscript, two groups have also reported an association of TRAF1 and TRAF2 with the 4-1BB cytoplasmic domain using yeast 2 hybrid analysis (49, 50). Arch and Thompson (49) demonstrated TRAF1 and TRAF2 association with the murine 4-1BB cytoplasmic domain. In addition, using a fusion protein between the murine 4-1BB cytoplasmic domain and the transmembrane/extracellular domains of CD28, Arch and Thompson found constitutive association of 4-1BB with TRAF 2 in HEK293 cells (49). Jang et al. have reported that TRAF1 and TRAF2 associate with human 4-1BB and they find that when full-length 4-1BB and the TRAF proteins are overexpressed in 293 these associations are observed in the absence of 4-1BB receptor cross-linking (50). The difference between those studies and our results may reflect the higher level of proteins expressed in the 293 cells versus our use of endogenously expressed molecules in T cells.
In this report we have also shown that resting T cells isolated from mice that lack TRAF2 or express a dominant
negative form of TRAF2 in their lymphoid subset fail to
respond to 4-1BB signaling. TRAF2 mice have very small
lymphoid organs and die at 2-3 wk of age, whereas mice
expressing a TRAF2 DN transgene in their lymphoid cells
are viable and have enlarged lymphoid organs due to the presence of an abnormal number of B cells. In spite of these
marked phenotypic differences, both types of TRAF2-deficient mice have demonstrated an essential role for TRAF2
in JNK activation in response to TNF (35, 36). Similarly,
in this report we have demonstrated that the two different
types of TRAF-deficient mice have very similar outcomes
for the T cell response to 4-1BBL. That is, responses to
anti-CD28 were retained, whereas responses to 4-1BBL
actually went from being stimulatory in wild-type mice, to
being inhibitory to anti-CD3-induced IL-2 production in
TRAF2-deficient mice (Figs. 8 and 9). The observation
that signaling via 4-1BB is actually slightly inhibitory to
anti-CD3-stimulated T cells is unexplained. It is possible
that this result is due to an inhibitory signal from 4-1BB
that is normally masked by the positive signaling effects of
TRAF2.
For both TRAF2 DN mice and TRAF2 mice, we also
observed an increase in background response to anti-CD3
alone. The reason for this increase in background is not
clear. The purity of T cells from TRAF2 DN mice or their
transgene-negative littermates was indistinguishable and addition of CTLA-4 Ig to block effects of B7 family molecules had no effect on the response (data not shown). Thus,
the increased response of isolated resting T cells to immobilized anti-CD3 does not appear to be due to the presence
of contaminating B7-expressing APCs. Lee et al. have
shown that the response of TRAF2 DN lymph node T
cells to anti-CD3 presented by accessory cells is decreased
compared with transgene-negative littermates (35). The
data in our report, showing a slight decrease in the response
of lymph node TRAF2 DN T cells to anti-CD3 and anti-CD28, agrees with that finding. However, the increased
response to anti-CD3 alone (Figs. 8 and 9) is puzzling. Yeh
et al. have shown that serum levels of TNF are increased in
TRAF2-deficient animals and have suggested that lack of
TRAF2 leads to dysregulation of TNF synthesis (36). It is
possible that the dysregulation of TRAF2 prevents feedback inhibition of the expression of other molecules produced by or expressed on the purified T cells, and that
these in turn allow enhancement of the response to anti-CD3 alone.
In addition to associating with TRAF2 (Figs. 6 and 7), the 4-1BB cytoplasmic tail can also associate with TRAF1 in vitro. The biological significance of this finding remains to be tested. In contrast to the results with TRAF2-negative mice or with mice expressing a dominant negative form of TRAF2, T cells from mice that overexpress wild-type TRAF1 showed no perturbation of responses to anti-CD3 plus anti-CD28 or s4-1BBL (data not shown). These results are not surprising in view of the data from Speiser et al., which showed that T cells from the TRAF1 overexpressing mice have normal proliferative responses but are resistant to antigen-induced apoptosis (38). This lack of perturbation of 4-1BB signaling by overexpression of TRAF1 could be due to the dispensability of TRAF1 for 4-1BB costimulatory signals or could be because the amount of TRAF1 recruited during 4-1BB signaling in wild-type mice is not limiting. It was also possible that overexpression of TRAF1 would interfere with signaling via TRAF2 by forming heterodimers that prevent other interactions of the TRAF2 molecule, but this did not seem to occur, as 4-1BB signaling was neither impaired nor enhanced by overexpression of TRAF1 (data not shown).
In summary, the results presented here show that 4-1BBL can function in isolation to costimulate T cell responses that are independent of the CD28 molecule. When signaling through the TCR is strong, 4-1BB signaling is as effective as anti-CD28 signaling in stimulating IL-2 production by T cells. In contrast, at low anti-CD3 doses, the CD28 costimulatory pathway seems to be more effective than the 4-1BB costimulatory pathway. The induction of IL-2 in response to the 4-1BB costimulatory signal is dependent on the presence of a functional TRAF2 molecule. Given the importance of TRAF2 in JNK activation in response to CD40L or TNF, a plausible model is that synergistic activation of JNK by TCR plus 4-1BB via TRAF2 leads to high level IL-2 gene transcription and in this way the 4-1BB molecule can replace the CD28 molecule in resting T cell activation. Thus, our data add to the accumulating body of evidence for a pivotal role for the TRAF2 molecule in signaling by TNFR family members.
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Footnotes |
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Address correspondence to Dr. Tania H. Watts, Department of Immunology, University of Toronto, Toronto, ON M5S 1A8, Canada. Phone: 416-978-4551; Fax: 416-978-1938; E-mail: tania.watts{at}utoronto.ca
Received for publication 18 December 1997 and in revised form 2 April 1998.
We thank Chris Richardson for advice on the baculovirus expression system and for providing the pETL-HA vector, Sf 9, and Hi5 insect cell lines. We thank Rob Dunn for providing the MAG signal sequence-containing vector, and Pam Ohashi for helpful suggestions.
This work was funded by a grant from the Medical Research Council of Canada (MRC) to T.H. Watts, by a grant from the National Cancer Institute of Canada (NCIC) to T.W. Mak, and by a grant from the Howard Hughes Medical Institute to Y. Choi; K. Saoulli and M.D. Goldstein are funded by MRC studentships; W.-C. Yeh is funded by an MRC postdoctoral fellowship; and T.H. Watts is a Senior Scientist of the NCIC with funds from the Canadian Cancer Society.
1Abbreviations used in this paper
4-1BBL, 4-1BB ligand;
AP, alkaline phosphatase;
CT, cytoplasmic tail;
DN, dominant negative;
GST, glutathione
S-transferase;
HA, hemagglutinin;
ICAM-1, intercellular adhesion molecule 1;
JNK, c-Jun NH2-terminal kinase;
NF-B, nuclear factor-
B;
RT, reverse transcriptase;
s4-1BBL, soluble form of 4-1BBL;
TRAF, TNF receptor-associated factor.
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