Molecular cloning and sequence of Sparus aurata skeletal myosin light chains expressed in white muscle: developmental expression and thyroid regulation
1 Department of Biochemistry and Biotechnology, University of Thessaly, 26 Ploutonos Street, 41221 Larissa, Greece and
2 Centre of Marine Sciences (CCMAR), University of Algarve, Campus de Gambelas, 8000 Faro, Portugal
*Author for correspondence (e-mail: kmoutou{at}uth.gr)
Accepted June 13, 2001
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Summary |
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Key words: sea bream, Sparus aurata, myosin light chain, white muscle, development, thyroid regulation.
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Introduction |
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Both MHCs and MLCs exist in multiple isoforms that show tissue-specific and/or developmental-stage-specific distribution, and their expression is known to be under environmental and hormonal control (Whalen et al., 1981; Gauthier et al., 1982; Izumo et al., 1986; Yamano et al., 1994; Hill et al., 2000). As is the case for other vertebrates, fish skeletal muscle myosins contain two alkali light chains, MLC1 and MLC3, and a regulatory light chain, MLC2. Different isoforms have been isolated and characterised from skeletal muscle of fish in an attempt to understand their contribution to muscle growth and contractile properties and their regulation during development and by environmental factors such as temperature, diet and exercise (Perzanowska et al., 1978; Rowlerson et al., 1985; Ochiai et al., 1988; Johnston, 1994; Hirayama et al., 1997; Hirayama et al., 1998; Johnston et al., 1998; Xu et al., 1999; Hill et al., 2000). Their expression patterns in fish present further interest; skeletal muscle growth in fish occurs both by hyperplasia and by hypertrophy throughout much of their life cycle, in contrast to mammals, in which hyperplasia is restricted largely to the pre- and perinatal period (Johnston et al., 1998). In this way, fish provide a model for the study of muscle development and the mechanisms underlying muscle cellularity.
In mammals and birds, thyroid hormone has been reported to be one of the major factors that control the developmental transition of myosin isoforms (Gambke et al., 1983; Butler-Browne et al., 1984; Gardahaut et al., 1992). In fish, larval development has been positively correlated with the plasma triiodothyronine (T3) concentrations of prespawning females and the amount of maternal thyroid hormones transferred into fish eggs (Ayson and Lam, 1983; Brown et al., 1988; Brown et al., 1989). More interestingly, thyroid hormone has been shown to hold a key role in the metamorphosis of the larvae of Japanese flounder Paralichthys olivaceus, regulating the transition of the DTNB-removable light chains (MLC2) from the larval to the adult type (Yamano et al., 1994).
In this study, we report the molecular cloning and sequence of skeletal myosin light chains 2 and 3 of gilthead sea bream Sparus aurata. The tissue and developmental expression of MLCs is described. Hyper- and hypothyrodism were induced by the administration of triiodothyronine (T3) or thyroxine (T4) and the hypothyroidal drug thiourea, respectively, and the effects of thyroidal status on the expression levels of MLCs in the white muscle were examined in relation to age.
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Materials and methods |
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Treatment with thyroid hormones
The effects of thyroidal state on the levels of expression of the two MLCs and the differential effects of age were determined in sea bream of two different ages after administering T3, T4 or the hypothyroidal compound thiourea (an inhibitor of the synthesis of endogenous thyroid hormone) (Yamano et al., 1994). 48 gilthead sea bream of mass 329g±9.77g (adult) and 48 fish of mass 7.35g±0.36g (juvenile) (means ± S.E.M.) were allocated to eight experimental groups that contained 12 adult or juvenile fish each. The groups were kept indoors in separate tanks and were fed once a day ad libitum. The water temperature during the experiments ranged between 26 and 27°C and the photoperiod was 12h:12h L:D.
On day 1 of the experiment, one group of each age class (adult or juvenile) was administered T3, T4 or thiourea, as a single intraperitoneal injection using coconut oil as a carrier at a ratio of 10µl coconut oil:1gbodymass. The doses used were: T3, 1µgg-1bodymass; T4, 1µgg-1bodymass; thiourea, 5ngg-1bodymass. In control fish, 10µl coconut oilg-1body mass was administered alone.
Fish were sampled for determination of the levels of MLC2 and MLC3 transcripts on days 2, 3, 5 and 8 of the experiment. On each occasion white muscle from three fish of each group was sampled from the area under the dorsal fin. The muscle sample was dipped immediately in chilled TRI reagent (Sigma, St Louis, MO, USA) and homogenised. Total RNA was extracted within 24h.
The efficacy of these treatments was assessed by measuring plasma T4 and T3 levels on the second and third days of the experiment. Blood samples were obtained from three individuals per treatment from the caudal vasculature, and plasma was separated by centrifugation and stored at 20°C until assayed. Plasma T3 and T4 levels were determined by radioimmunoassay (RIA) (as described by Power et al., 2000).
Generation of an homologous myosin light chain cDNA probe by reverse transcriptionpolymerase chain reaction (RTPCR)
Myosin alkali light chain 3 (MLC3) and regulatory light chain 2 (MLC2) cDNAs were cloned by using degenerated primers to amplify a 406base-pair (bp) and a 511bp fragment respectively. PCR primers were designed within the most highly conserved regions of both MLC2 and MLC3, identified after multiple sequence alignments of all MLC sequences available (GenBank). A forward primer based on a highly conserved region (EEFKEA), common to both light chains, was synthesised (5' GARGARTTYAARGARGC 3'; Pharmacia Biotech, Uppsala), and the reverse primer was 5' GCYTCRTARTTRATRCANCCRTT 3'.
cDNA was synthesised from 1µg of white muscle total RNA and PCR was carried out in a reaction (50µl) containing 5µl of white muscle cDNA, 10mmoll-1 Tris-HCl, pH9.0, 50mmoll-1 KCl, 0.1% Triton X-100, 2.5mmoll-1 MgCl2, 1mmoll-1 each deoxynucleotide triphosphate, 200pmol of forward primer, 200pmol of reverse primer and Taq DNA polymerase (5 units; Promega, Madison, WI, USA). The cDNA template was amplified after an initial denaturing step at 94°C for 2min, using 20 cycles of the following PCR protocol: 94°C for 1min, 50°C for 2min and 72°C for 1min. PCR products corresponding to the expected size were cloned using the pGEM-T Easy cloning system (Promega) and sequenced. Two of the cloned and sequenced PCR products were subsequently used as probes to screen a sea bream larva cDNA library for MLC2 and MLC3.
cDNA library screening
A sea bream larva cDNA library was constructed in the vector Lamba ZapII with reverse-transcribed cDNA obtained from 5µg of poly(A)+ mRNA, obtained from total RNA by chromatography on columns of oligo-dT cellulose. Homologous probes for MLC2 (511bp) and MLC3 (406bp), generated by RTPCR, were used to screen the library under high-stringency conditions. Duplicate membranes (Hybond-C, Amersham) were hybridised with the [-32P]dCTP-labelled probe overnight at 65°C. Stringency washes were carried out at 65°C with 0.1x SSC (1x SSC is 0.15moll-1 sodium chloride, 0.015moll-1 sodium citrate) containing 0.1% SDS. Positive clones were isolated and excised from the ZapII vector into pBluescript SK(-) (Stratagene) and fully sequenced using the dideoxy chain termination procedure (Sanger et al., 1977) using a Licor DNA4200 automated sequencer (MWG Biotech, UK). Positive clones corresponding to each myosin light chain were sequenced three times.
Phylogenetic analysis
Previously published MLC sequences were obtained from the GenBank database and used for amino acid comparison and phylogenetic analyses. The following is the list of accession numbers for the skeletal MLC2 sequences. Mammalian: P41691, Felis catus superfast MLC2 (Qin et al., 1994); Q02045, Homo sapiens superfast MLC2 (Collins et al., 1992); P24732, Oryctolagus cuniculus (Maeda et al., 1990); P04466, Rattus norvegicus (Nudel et al., 1984); P97457, Mus musculus (Palermo et al., 1995); Avian: P02609, Gallus gallus (Suzuyama et al., 1980); Piscine: AAF71271, Oncorhynchus kisutch (Hill et al., 2000); AAC32193, Danio rerio (Xu et al., 1999); AAF00097, Danio rerio cardiac MLC2 (Yelon et al., 1999); BAA95142, Engraulis japonicus; BAA95140, Sardinops melanostictus; BAA95125, Thunnus thynnus; BAA95128, Euthynnus pelamis; BAA95131, Pennahia argentata; BAA95134, Cypselurus agoo; BAA95137, Trachurus trachurus; BAA89705, Cyprinus carpio (Hirayama et al., 1998).
The following is the list of accession numbers for the skeletal MLC3 sequences. Mammalian: NP000249, Homo sapiens (Cohen-Haguenauer et al., 1989); CAA37977, Oryctolagus cuniculus (Muller et al., 1990); P02601, Rattus norvegicus (Strehler et al., 1985); P05978, Mus musculus (Robert et al., 1984); CAA64353, Sus scrofa (Davoli et al., 1997); Q60605, Mus musculus non-muscle MLC3 (Hailstones and Gunning, 1994); Avian: P02605, Gallus gallus (Nabeshima et al., 1982); Piscine: BAA94860, Danio rerio; BAA95139, Sardinops melanostictus; BAA95124, Thunnus thynnus; BAA95127, Euthynnus pelamis; BAA95130, Pennahia argentata; BAA95133, Cypselurus agoo; BAA95136, Trachurus trachurus; BAA12733, Cyprinus carpio (Hirayama et al., 1997).
The amino acid sequences were aligned using the Clustal W program (Thompson et al., 1994). Evolutionary distances were estimated using Kimuras empirical method for proteins (Kimura, 1983), and phylogenetic trees were constructed by the neighbour-joining method (Saitou and Nei, 1987) using PHYLIP (Felsenstein, 1993). 100 bootstrap analyses were performed for the phylogenetic analysis.
Northern blot analysis
Northern blot analysis was performed to determine the expression of the MLCs in a variety of tissues of adult sea bream and at different developmental stages and to check differential expression in white muscle following administration of T4, T3 or thiourea. To assess the effects of T3, T4 or thiourea treatment on the expression of the different forms of MLC, densitometry was carried out on the resulting autoradiograph for each probe and results were normalised against the expression of cytoplasmic ß-actin, expression of which was unmodified by the various treatments. Exposure times of 30min, 1h, 2h and 3h were tested to ensure that the time chosen for densitometric quantification (30min) fell within the linear range of the photographic technique.
Total RNA (5 µg) from a variety of tissues of adult sea bream, from whole fertilised eggs at different developmental stages and from white muscle of untreated sea bream and sea bream treated with T4, T3 or thiourea was fractionated on a formaldehyde gel and transferred to Hybond-N nylon membranes (Amersham) with 10x SSC and crosslinked at 80°C for 2h. Hybridisation was performed sequentially with [-32P]dCTP-labelled full-length MLC2 and MLC3 probes. Membranes were hybridised first with ß-actin, then with MLC2 and finally with MLC3. The order of probing the membrane with MLC2 or MLC3 did not appear to affect the outcome of the results. The first probe hybridised to the membrane was always ß-actin. Film was exposed over the time range indicated and used for densitometry. Subsequently, membranes were washed with hybridisation solution, pre-hybridised and hybridised to MLC3 or MLC2. Stringency washes were carried out at 60°C with 1x SSC containing 0.1% SDS. Film was exposed to membrane, and densitometry analysis was carried out on the resulting film. The same procedure was carried out for the final probe (MLC2 or MLC3). The final film exposed contained all the signals (since membrane was not stripped between probing) but was used to scan by densitometry only the transcript corresponding to the probe used. The consistency of RNA loading for the northern blot had previously been checked by fractionation of samples on mini-agarose gels and observing the intensity of 18S and 28S ribosomal RNA bands after staining with ethidium bromide. Three different northern blots were conducted with the same samples to ensure reproducibility of the technique.
Statistical analyses
Results for northern blot analysis are expressed as arbitrary units of mRNA levels, means ± S.E.M. of four samples, normalised against the quantity of total RNA by ß-actin expression. Each sample consisted of a pool of the three individuals sampled on each experimental day. Two-way analysis of variance (ANOVA) was performed between fish of different age and between different treatments. One-way ANOVA was performed for plasma T4 and T3 levels and for the effects of thyroid hormones (T3 and T4) and thiourea on the expression of myosin light chains. To establish differences between means, an unpaired two-tailed Students t-test was used. Variables were considered significantly different at P<0.05 (Zar, 1996).
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Results |
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Effect of thyroidal status on the expression of myosin light chains
In control sea bream, both MLC2 (F=97.6, P<0.01) and MLC3 (F=127.8, P<0.01) transcripts were expressed at significantly higher levels in juvenile than in adult fish (Fig.6). The magnitude of expression was higher for MLC3 (8.8-fold) than for MLC2 (3.8-fold). The ratio of expression MLC3:MLC2 was also higher in juvenile fish (4.4-fold) than in adult fish (1.9-fold), indicating an age-related pattern of the relative expression of MLCs, due mainly to higher levels of MLC3 expression at a younger age.
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In juvenile fish, administration of T4 led to a significant increase in the level of expression of MLC2 (F=7.30, P<0.01), whereas it had no significant effect on the expression of MLC3 (Fig.6). MLC2 transcript levels had almost doubled 24h after T4 administration and remained elevated throughout the experimental period (Fig.6A). Neither T3 nor thiourea administration significantly affected the accumulation of MLC transcripts (Fig.6).
In adult fish, administration of T4 resulted in a slight, but not statistically significant, increase in expression of both MLC2 and MLC3 (Fig.6). A gradual accumulation of MLC3 transcripts followed the administration of T4 (Fig.6B). The highest value was reached on day 8. MLC2 expression followed almost the same pattern (Fig.6A). However, expression levels of both MLC2 and MLC3 were unaffected by the administration of thiourea or T3.
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Discussion |
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For MLC3, M. musculus non-muscle MLC3 was used as an outgroup (Fig.8). The phylogenetic analysis revealed two major clusters, one formed from the piscine forms and the other consisting of the mammalian forms. Chicken skeletal MLC3 was clustered together with the mammalian forms. The piscine clade was supported in 100% of bootstrap replicates.
Phylogenetic analysis and alignment comparisons revealed a high degree of conservation of MLC2 and MLC3 within vertebrates, with S. aurata sequences presenting 79% and 67% identity respectively to O. cuniculus. Moreover, in MLC2, the Ca2+-binding motif appeared to be the region of the protein best conserved. It has been suggested that MLC1, MLC2, MLC3 and troponin C evolved from a common ancestor and contained four similar Ca2+-binding domains (Qin et al., 1994; Holland et al., 1995), and that an original single Ca2+-binding domain underwent tandem duplications to give a protein with four such domains (Holland et al., 1995). Each of these domains, designated IIV from the N terminus, consists of a pair of helices and a central 12-residue Ca2+-binding site. Evolutionary substitutions in amino acid sequences have resulted in the loss of Ca2+-binding ability in some domains. As a result, troponin C and parvalbumin each have two functional domains, while MLC2 has only domain I (Fig.2). In MLC3, ancestral domain III appears to be evolutionarily the most conserved (Fig.4), with a degree of identity within the domain ranging between 82% and 100%. However, it is controversial whether any MLC3 (alkali) binds Ca2+ (Collins, 1991).
The high degree of conservation between the previously identified MLCs, which is also evident from the newly isolated MLC2 and MLC3, has led to considerable speculation about the evolution of MLC genes in animals (Barton and Buckingham, 1985; Barton et al., 1988; Hailstones and Gunning, 1990; Holland et al., 1995). Although it is generally agreed that MLC genes in vertebrates are derived from the duplication of an ancestral gene, divergent opinions exist about the nature of this ancestral gene. The observation that amphioxus has only one alkali MLC gene has been taken as evidence that duplications occurred after the amphioxus and vertebrate lineages diverged. Since the amino acid sequence of amphioxus alkali MLC is most like the vertebrate embryonic alkali MLC, it is proposed that the ancestral MLC1/3 was probably more like an embryonic form (Holland et al., 1995).
In gilthead sea bream, transcripts of the MLCs isolated could be detected only in white muscle. No other type of muscle expressed either of the MLCs. Individual fish muscle fibres can be categorised as fast or slow on the basis of physiological and molecular criteria that include the expression of different myosin isoforms. The slow and the fast muscle fibres of fishes occupy distinct regions of the body muscle and are involved in different physiological functions. The white, fast glycolytic muscle constitutes the trunk musculature and is specialised for short forceful bursts of activity. Since, in striated muscle, MLC3 content has been positively correlated with the rate of actin filament movement (Lowey and Trybus, 1995), the exclusive expression of MLCs in the white muscle is probably in the context of differential expression profiles of myosin isoforms, which contributes to the functional specialisation of fast muscle with their mechanical and moving properties, implying the existence of a fibre-specific regulatory mechanism of their gene expression.
During development in sea bream, the onset of MLC2 expression was coincident with the start of somitogenesis. MLC3 expression occurred later, when somitogenesis was well advanced. The early appearance of MLC2 transcripts during sea bream development may indicate the absence of embryonic forms of this mRNA. However, another MLC2 transcript of smaller size was detectable later in development, and this form may represent either a splicing variant or another MLC2 isoform, which might contribute to the formation of myosin molecules with different contractile properties. In contrast, MLC3 transcripts were detected at the later stages of segmentation, and further work is required to determine whether MLC3 replaces an earlier embryonic form.
The different experimental thyroidal states induced in the present study affected the expression levels of MLC2 only, which is in agreement with the myosin light chain transition pattern observed in metamorphosing flounder (Paralichthys olivaceus) after administration of thyroid hormone (Yamano et al., 1994). Moreover, the results for the expression of MLC2 in juvenile and adult sea bream suggest that the effects of altered thyroidal status were age-related. Thyroid hormone is known to reverse substantially at least some of the changes that occur in ageing muscle (Li et al., 1996), while the capacity for myosin heavy chain (MHC type IIA) isoform switching in response to T3 treatment has been reported to be age-related in rats (Larsson et al., 1995). However, the differential response or lack of response of the two MLCs to varied thyroidal states indicates the existence of different regulatory mechanisms of gene expression in response to thyroid hormones.
In higher vertebrates, it is well established that thyroid hormone induces the foetal-to-adult transition of skeletal muscle MHCs (Gambke et al., 1983; Adams et al., 1999). Thyroid hormones exert their major effects by binding to nuclear receptors (TRs) that act as DNA-binding transcription factors. TRs bind to DNA sequences known as thyroid-hormone-responsive elements (TREs) and found in the regulatory regions of target genes and, according to the nature of the TRE, gene expression may be enhanced or inhibited (Wu and Koenig, 2000). In the case of sea bream MLC2, one possibility is that thyroid hormone turns on the first step of a cascade process involving other critical developmental and myogenic factors (e.g. IGF-I, myogenin), which finally activate or inactivate the MLC2 gene, indicating that the MLC2 gene lacks a TRE (Muscat et al., 1995).
Another possibility is that thyroid hormone and its receptor act directly on a TRE present on the MLC2 gene. The latter explanation, however, is not sufficient to support the age-related pattern of response observed in the present study. During amphibian metamorphosis, mRNAs for thyroid receptors are expressed at high levels in the nervous system but at low levels in muscle (Kawahara et al., 1991). Thus, the thyroidal control of MLC2 via nervous stimulation may be a possible cascade regulatory process. Furthermore, the recent evidence that the regulatory action of thyroid hormone on myosin gene expression is exerted via different type of TR indicates that, in addition to the presence of a TRE on the myosin gene, the expression of the appropriate TR might be a prerequisite for regulation of the gene transcription (Yu et al., 2000). TRs have been found to be differentially expressed in different tissues during fish development (Yamano and Miwa, 1998). Thus, myosin gene responsiveness to thyroid hormone might even be regulated at the receptor level.
In summary, two skeletal myosin light chains, MLC2 and MLC3, expressed in the white muscle only of gilthead sea bream, were isolated. Both forms were expressed in sea bream embryos, MLC2 at the start of segmentation and MLC3 in the latter stages of this process. The expression of the isolated MLCs was affected by thyroid hormone status. However, the two MLCs had different expression profiles as well as age-related responsiveness to experimentally induced thyroidal states, suggesting that their mechanisms of regulation by thyroid hormones are different.
The sequence data reported in this paper have been submitted to EMBL/GenBank data libraries under accession numbers AF150904 (MLC2) and AF149756 (MLC3).
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Acknowledgments |
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