Epaxial muscle function in trotting dogs
1 Biology Department, Heidelberg College, 310 E. Market Sreet, Tiffin, OH 44883, USA,
2 Department of Geology, Bryn Mawr College, 101 N. Merion Avenue, Bryn Mawr, PA 19010, USA and
3 Department of Biology, 201 South Biology, University of Utah, Salt Lake City, Utah 84112, USA
*Author for correspondence at present address: Department of Ecology and Evolutionary Biology, Box G-BMC204, Brown University, Providence, RI 02912, USA (e-mail: Dale_Ritter{at}Brown.edu)
Accepted May 31, 2001
![]() |
Summary |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Key words: epaxial muscle, electromyography, locomotion, dog, trotting.
![]() |
Introduction |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Mammals, especially those described as cursors, are believed to possess an adaptive locomotor complex that includes parasagittal limb orientation and associated movement of the trunk in the sagittal plane (Rockwell et al., 1938; Gray, 1968; Rewcastle, 1981). The selective advantages attributed to this configuration include increased speed, endurance, acceleration and maneuverability; all factors that are presumably important in ecologically relevant activities such as migration, intraspecific competition and predator-prey interactions (Hildebrand, 1995). The epaxial muscles of mammals form an integral part of this locomotor complex. For example, the role of the epaxial muscles in producing sagittal movements of the body axis is a critical component of galloping, a high-speed gait that is only observed in mammals (with the notable exception of some crocodilians). These sagittal movements, in addition to coordinating limb movements, are estimated to increase the top speed of a sprinting cheetah by 6 miles per hour (Hildebrand, 1961).
Several studies have addressed the locomotor function of the epaxial muscles during walking and trotting in mammals. These studies have focused on a single site on the trunk (lumbar site of cats: English, 1980; Carlson et al., 1979), only one of the epaxial muscles (longissimus dorsi muscle: Tokuriki, 1974), or on animals that do not exhibit a typical quadrupedal gait (e.g. primates: Thorstensson et al., 1982; Carlson et al., 1988; Shapiro and Jungers, 1988; Shapiro and Jungers, 1994). In all cases, these studies have concluded that the epaxial muscles support or stabilize the trunk during walking and trotting. Not surprisingly, the specific aspect(s) of the locomotor forces against which the epaxial muscles stabilize the trunk, has not been elucidated. A muscle that stabilizes the body (i.e. a postural muscle) will, by definition, resist movement. Resistance of movement, if successful, will result in a lack of movement. Therefore the specific function(s) of a stabilizing or postural muscle is very difficult to see, and even more difficult to test.
This study combines a thorough description of the activity of the epaxial muscles in trotting dogs with an attempt at a more detailed functional analysis of these muscles. Trotting was studied because it appears to be the basal running gait for terrestrial vertebrates. It is present in salamanders, lizards and mammals, and therefore provides a means of comparison among tetrapods that should facilitate our understanding of the evolution of both terrestrial locomotion and of epaxial muscle function in vertebrates.
![]() |
Materials and methods |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Muscular anatomy
We verified the anatomy of the epaxial muscles through dissection of cadavers. A brief description of the epaxial muscles (condensed from Evans, 1993) is given below.
The most medial epaxial muscle we recorded from was the multifidus, one of the transversospinalis muscle group. The multifidus is a segmental muscle, the individual segments of which originate from the articular, transverse or mammillary process of a vertebra, generally span two vertebrae, and insert on the spinous process of a cranial vertebra. There are four portions of the multifidus, named according to the region of the trunk they are associated with. We implanted electrodes in the multifidus lumborum and the multifidus thoracis.
Lateral to the multifidus is the longissimus dorsi muscle, the most robust of the epaxial muscles. The longissimus dorsi is composed of overlapping muscle fascicles of variable length that extend from the iliac crest to the skull. The longissimus dorsi is divided into the thoracolumbar, cervical and capital regions. The thoracolumbar region may be further divided into a longissimus lumborum and a longissimus thoracis, and these are the two regions we recorded from. Fascicles of the longissimus lumborum originate from the ileum and insert on the accessory processes of the first to sixth lumbar vertebrae. Fascicles of the longissimus thoracis also originate from the ileum, and end in bifurcating tendons that attach to the caudal borders of the sixth to thirteenth ribs, and the accessory processes of the sixth to thirteenth thoracic vertebrae.
The most lateral of the epaxial muscles is the iliocostalis, which also consists of multiple fascicles and may be divided into a lumbar and thoracic region. The iliocostalis lumborum arises from the ileum, and consists of fascicles that attach to the tenth through the thirteenth ribs. Fascicles of the iliocostalis thoracis arise from the cranial aspect of the vertebral ends of the ribs. The fascicles form a common belly, and from this muscle belly tendons arise which insert on the caudal aspects of the ribs and on the transverse process of the seventh cervical vertebra.
Electromyography
Electrical activity of the epaxial muscles was recorded from two dogs trotting on a motorized treadmill, and from two dogs trotting on a stationary trackway. Each dog underwent two surgical implantations of 1216 EMG electrodes. Two epaxial sites on the trunk were implanted with electrodes. The lumbar site was approximately mid-way between the crest of the ilium and the last rib, at the level of the fourth lumbar vertebra. The thoracic site was at approximately the level of the sixth thoracic vertebra. Recording of muscle activity began on the second day after surgery and continued for 25 days. 58 days after implantation the electrodes were removed. The dogs recovered for at least 30 days before the second electrode implantation. All procedures conformed to the guidelines of the Institutional Animal Care and Use Committees at Brown University and the University of Utah.
For surgery, subjects were initially anesthetized with an intravenous injection of Pentethal or Propofol, to effect. They were intubated with an endotracheal tube and maintained on a ventilator with oxygen to 1.3 minimum alveolar concentration and 1.52.0% Isoflurane for the duration of the surgery. Incisions were made through the skin above the site of electrode placement and patch (Loeb and Gans, 1986), or sew-through (Betts et al., 1976) electrodes were secured to the muscles of interest. Lead wires from the electrodes were passed subcutaneously to a dorsal exit point just caudal to the dorsal tips of the scapulae. Electromyographic signals were passed through shielded, light-weight cables (Cooner Wire, Inc.), filtered above 1000Hz and below 100Hz, and amplified 5,000 or 10,000 times with Grass P511 AC amplifiers. Data were stored in digital form at 2000Hz on a PC or Macintosh computer.
Patch electrodes were constructed from 0.3mm, multistranded, Teflon-insulated stainless steel wire (Cooner Wire Inc.) sewn through 1cm x 2cm rectangles of 0.8mm Silastic sheeting (Dow Corning). The exposed portions of the wires were parallel to one another, approximately 1mm in length, separated by 12mm, and oriented at 90° to the fiber direction of the muscle. Sew-through electrodes were constructed from the same wire as the patch electrodes. An overhand knot was tied in one end of the two leads. A 12mm length of the insulation was removed from one side of each wire. These uninsulated segments were staggered approximately 6mm and 8mm from the knot. A small square (3mm x 3mm) of Silastic sheeting was threaded past the exposed portion of the wires to abut the knot. The two lead wires were sewn directly into the muscle, parallel to the muscle fibers, so that the exposed segments of the wires were inside the muscle and the Silastic button acted as a stop. A second Silastic button was then threaded to the point where the wires exited the muscle and secured in place with a square knot in the leads. This provided a secure anchoring of the electrode and the 12mm staggering of the exposed segments parallel to the fiber orientation resulted in a dipole when the muscle was active.
The general pattern of muscle activity was determined by averaging (Banzett et al., 1992; Carrier, 1996) of the electromyographic signals (Fig.1). For the data from the two dogs running on the treadmill, the beginning and end times of individual cycles were determined from accelerometer signals (an analog signal of the locomotor cycle was obtained from the two treadmill dogs by monitoring the vertical acceleration of the trunk with an Omega 103 accelerometer, mounted dorsally in the lumbar region). These times were used to divide the electromyographic signals into locomotor cycle segments. The electromyographic segments were then rectified, partitioned into 100 bins of equal duration, and a measure of EMG intensity was calculated for each bin by multiplying the number of spikes by the average amplitude of the spikes (EMG energy of Gans and Gorniak, 1980). The EMG activity was then averaged on a bin by bin basis for 20 consecutive locomotor cycles. The resulting trace represents the average EMG activity during an average locomotor cycle. For the data from the two dogs that ran on the stationary track, the beginning and end times of individual cycles were determined using footfalls, taken from videotapes. Because these animals were only in view of the camera for one or two locomotor cycles, single cycles from multiple trials were averaged to produce the mean EMG activity traces.
|
Force-plate recordings
Single limb ground forces were measured in the two dogs that ran on the trackway. The trackway was carpeted, 35m long, and had a force plate (Kistler, 9281B SN) located mid-track that was mounted flush with the track floor. The resultant of the single limb ground reaction forces, from sample trials, was combined with video recordings of the same trials to visualize the ground reaction force vectors relative to the trunk and girdles. Fore- and hindlimb force data were combined to produce an average force profile for the support phase of the trot. Recordings from 20 strides were used to generate average ground forces for each dog.
Loading experiments
To test an emergent functional hypothesis (described below) for the activity of the epaxial muscles during trotting, we conducted experiments in four dogs in which the trunk of the dog was loaded with 815% of body mass. The dogs carried the added mass in saddlebags on their backs in two different configurations. In one case, all of the added mass was carried mid-trunk at the level of 11th thoracic vertebrae. In the other configuration, half the added mass was carried over the pectoral girdle and the other half was carried over the pelvic girdle. Activity of the longissimus dorsi muscle was recorded from each of these dogs as they trotted on the treadmill in three conditions; without the mass, with the added mass positioned over the girdles, and with the added mass positioned over the center of the trunk. EMG signals were filtered above 1000Hz and below 100Hz, sampled at 4000Hz, and stored on a Macintosh computer. The effect of the two manipulations was evaluated by calculating the mean EMG activity (integrated area of the rectified signal) from 20 locomotor cycles from each condition. For each dog, unpaired t-tests were performed to compare the mean EMG activity from the unloaded, girdle-loaded and mid-trunk loaded conditions.
The hypothesis tested by this experiment was that the epaxial muscles function during trotting to resist the tendency for the trunk to rebound (flex) vertically during the second half of limb support (described below). The mid-trunk weight increased the gravitational bending moment on the trunk, and therefore should also increase the rebound of the trunk. If this was true, the mid-trunk loading manipulation would be expected to require greater muscle activity from the muscles that stabilize the trunk against the rebound. The girdle weights should not have increased the gravitational bending moment of the trunk.
Because the hypothesis is one of postural control we do not have a prediction of the extent to which amplitude of flexion would be influenced by the added mass. If we were to observe no change in trunk flexion, but an increase in epaxial activity with the mid-trunk load, we would argue that the epaxial muscles increased activity to effectively control the increased tendency for rebound. Similarly, if we were to observe an increase in trunk flexion and an increase in epaxial muscle activity, we would conclude that the epaxial muscles acted to control the rebound, but were unable to fully correct for the added mass. Stated another way, if the amplitude and velocity of the rebound remained exactly the same in the mid-trunk manipulation, the postural muscles would still be required to exert greater force because the momentum of the mid-trunk would be greater due to the added mass. Hence, we did not monitor the amplitude of trunk flexion and extension in this experiment.
![]() |
Results |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Multifidus
The multifidus muscle was very consistent in exhibiting two bursts of activity per locomotor cycle (biphasic activity), with each burst occurring during the second half of the support phase of a pair of limbs (Fig.2, Fig.3). Intensity differences between primary and secondary bursts were especially apparent at the lumbar site (Fig.3). At the lumbar site the primary burst occurred during the second half of the support phase of the ipsilateral hindlimb, and the duration of activity averaged 33% of one locomotor cycle. The secondary burst occurred during the second half of the support phase of the contralateral hind limb, and was of shorter duration than the primary burst (averaged 18% of one locomotor cycle). The pattern was very similar at the thoracic site, though the primary burst duration averaged 27% of cycle duration, while the secondary burst averaged 18%. At both sites, the midpoint of activity of the secondary burst occurred approximately 50% of cycle time after the midpoint of activity of the primary burst. This means that there is a period of bilateral activity (overlapping activity of left and right side muscles) during the second half of the support phase of each diagonal pair of limbs, and a period of no EMG activity during the first half of the support phase of each diagonal pair of limbs. This was a very repeatable pattern of activity, regardless of the individual dog recorded from.
|
|
Iliocostalis
Activity of the iliocostalis muscle was grossly different from both the multifidus and longissimus dorsi activity patterns (Fig.2, Fig.3). The thoracic site showed a consistent uniphasic pattern. This activity occupied approximately the second half of the ipsilateral hindlimb support phase, and averaged 30% of cycle duration. The lumbar site exhibited a biphasic pattern. The primary burst duration averaged 30% of cycle duration and occurred during the second half of ipsilateral hindlimb support. Although the secondary burst was consistently present at the lumbar site, it was of especially short duration, averaging only 6% of cycle duration. In one of the dogs (Fig.3A), the midpoint of activity of the secondary burst occurred 65% of cycle duration after the midpoint of activity of the primary burst. This means that in that dog in the lumbar region there was no period of bilateral activity of the iliocostalis muscle. A primary burst of activity ipsilateral to hindlimb support was immediately followed by a secondary burst of activity contralateral to that hindlimb, but the primary and secondary bursts of opposite sides did not overlap. In the other dogs, however, the midpoint of the secondary burst occurred 50% of the cycle duration after the midpoint of the primary burst, meaning that in these dogs there is a period of overlapping activity of the iliocostalis muscle in the lumbar region.
Ground reaction forces and vectors
Both dogs showed a very regular pattern of ground reaction forces. A combined front/rear mean force trace is first described relative to limb support (Fig.4). The vertical component of the ground forces exhibited a regular increase during the stance phase of a given pair of limbs, peaked slightly before mid-stance, and then showed a regular decrease towards zero during the second half of the stance phase. The foreaft component of the ground forces was divided into a deceleration and an acceleration phase. Deceleration occurred during the first part of the stance phase. There was a regular increase in force that peaked at approximately 20% of the stance phase, then decreased back to zero. At approximately the time of peak vertical force the foreaft force became positive (becomes an acceleration), peaked at approximately 70% of the stance phase, and then decreased back to zero. The third component of the ground forces is the lateral force, which was very small in our recordings and is not considered in this study.
|
|
|
|
|
|
|
![]() |
Discussion |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Do the epaxial muscles extend the back during trotting? Extension occurs during approximately the first half of the support phase of a given pair of limbs (Fig.7). Conversely, epaxial EMG occurs during approximately the second half of the support phase of a given pair of limbs (Fig.2, Fig.3). Therefore, the epaxial muscles are active throughout flexion of the back. Because the muscles are active during a movement they cannot produce, we conclude that the epaxial muscles are not actively producing sagittal movement of the trunk.
In addition to producing movement (performing work) during locomotion, a muscle may also oppose a movement (provide postural support). For example, the left iliocostalis muscle could be activated and shorten to produce lateral flexion such that the left side of the trunk becomes concave. Alternatively, the left iliocostalis muscle might be activated in order to resist forces that would cause the left side of the trunk to become convex. In fact, the usual function ascribed to the epaxial muscles of mammals, at least during walking and trotting, is that of stabilization of the trunk, and stabilization implies resistance of movement, as opposed to production of movement. We may look to the ground reaction forces and their likely effects to evaluate the possibility that the epaxial muscles function to directly counteract some component of the ground reaction forces.
The ground reaction forces that dogs produced while trotting can be divided into three components, based on their orientation to the dog. Lateral forces are those that act perpendicular to the direction the animal is moving in. The magnitude of these forces in dogs is very small (Fig.4), and we do not consider them in this study.
Foreaft forces act in the same direction that the animal is moving. Foreaft force production during the support phase can be divided into deceleratory and acceleratory phases, with deceleration occurring during the first half of support and acceleration occurring during the second half (Fig.4). What is the likely effect of these forces? Consider an animal supported by the right fore and left hind foot. During deceleration there is a rearward-directed force acting on the right side of the pectoral girdle and the left side of the pelvic girdle. These forces would tend to produce a lateral bending moment such that the left side of the trunk would become convex. In order to counteract this lateral bending moment the epaxial muscles ipsilateral to the rear foot would need to be active during the early part of support. Muscle activity does occur ipsilateral to hindlimb support, but the activity does not begin until mid-support (Fig.5), too late to effectively counter lateral bending of the trunk induced by deceleration. During the second half of the support phase the foreaft force produces forward acceleration, resulting in a forward-directed force at each limb girdle. The net effect of the acceleratory forces would be a lateral bending moment such that the right side of the trunk would end up convex. In order to counteract this bending moment the epaxial muscles would need to be active contralateral to hindlimb support. While there is some activity contralateral to hindlimb support, the primary burst of activity is ipsilateral, and the timing of onset and peak of EMG is somewhat later than might be expected for counteracting lateral bending induced by forward acceleration (Fig.5). Therefore the epaxial muscles do not appear to be counteracting lateral bending movments induced by the foreaft component of the ground forces.
The third component of the ground reaction force is the vertical force, the force that counteracts gravity. The net effect of the vertical force on the trunk will depend on the orientation of the ground reaction force vector relative to the limb girdles. Vectors oriented between the two girdles (caudal to the pectoral and cranial to the pelvic girdles) will tend to cause both flexor and extensor moments at the intervertebral joints. Conversely, vectors oriented cranial to the pectoral girdle and caudal to the pelvic girdle would produce bending moments that would tend to cause extension of the trunk. If the vectors pass through or close to the girdles the vertical force would also tend to cause extension of the back, though to a lesser degree than if the vectors passed cranial to the pectoral and caudal to the pelvic girdles. This last possibility, the force vectors passing close to the limb girdles, is what we see in the dogs (Fig.6). Therefore the vertical force should tend to cause extension of the back. Because the epaxial muscles are dorsal to the vertebral column, they cannot resist this tendency for back extension. Furthermore, the magnitude of the vertical force peaks at approximately mid-support, at which time the epaxial muscles have just become active (Fig.5). Maximum activity of the epaxial muscles occurs well after peak vertical force, as the magnitude of the vertical force is decreasing. Therefore the epaxial muscles do not appear to be involved in directly resisting the action of the vertical component of the ground forces.
Given that the epaxial muscles do not appear to directly stabilize the trunk against any component of the ground forces, nor to actively produce extension of the back, what is the function of these muscles? The timing of sagittal movements of the trunk suggests they are not initiated by axial muscles, but are incidentally produced by the ground reaction forces. During the first half of diagonal limb support in the trot, the mid portion of the trunk sags ventrally due to the force of gravity (i.e. the moments resulting from the vertical component of the ground force described above, Fig.7). In effect, dogs become slightly swaybacked during the first half of support. Then as the dog accelerates forwards and upwards during the second half of support, the back rebounds from the swayback position and undergoes sagittal flexion. As mentioned above, the epaxial muscles are anatomically positioned to produce extension or to resist sagittal flexion of the back, and it turns out that the epaxial muscles first become active at the time that the back begins to flex out of the swaybacked (extended) position. The muscles are active throughout back flexion, and slightly after the time of maximum flexion the epaxial muscles cease activity. Thus, the epaxial muscles, whose anatomy indicates they are likely to be extenders of the trunk, are active throughout flexion of the trunk.
These data led us to the hypothesis that the epaxial muscles function to counter sagittal rebound (flexion) of the trunk during the latter half of each trotting step. We refer to the cycle of sagittal extension and flexion during each step as sagittal bounce. During the first half of limb support, the vertical force causes the trunk to sag (extend) between the girdles. During the second half, the trunk rebounds vertically (flexes). We suspect this rebound is due to the recoil of elastic elements in the ventral body wall and the vertebral column, that are stretched during the first half of support by the vertical component of the ground forces. The epaxial muscles are active during the rebound phase, suggesting they reduce flexion of the trunk.
We tested this hypothesis with the loading experiments. We reasoned that the added mass, positioned mid-trunk, would increase the loading of the elastic elements, resulting in greater sagittal bouncing of the trunk as a dog trotted. Therefore, we expected greater activity of the axial muscles that are associated with resisting sagittal rebound. We expected that the same mass carried over the girdles would not alter the sagittal bounce of the trunk and would, therefore, serve as a control for the added mass. The results of the loading experiments support our hypothesis (Table1, Fig.9). Adding mass over the girdles resulted in either no increase in the activity or a decrease in activity of the longissimus dorsi muscle. In all four dogs, however, placing the added mass mid-trunk caused a significant increase in muscle activity.
Evolution of epaxial muscle function in terrestrial vertebrates
The data reported here provide information relevant to the evolution of epaxial muscle function in terrestrial vertebrates. First, our data reinforce a basic dichotomy between epaxial muscle activity patterns in mammals versus ectothermic tetrapods (salamanders and lizards, specifically). While mammals typically exhibit biphasic activity patterns (Waters and Morris, 1972; Tokuriki, 1973a; Tokuriki, 1973b; Carlson et al., 1979; English, 1980; Thorstensson et al., 1982; Shapiro and Jungers, 1988; Shapiro and Jungers, 1994), salamanders and lizards show uniphasic patterns (Frolich and Biewener, 1992; Ritter, 1995; Ritter, 1996). Our data also reinforce a basic difference observed between anamniote and amniote tetrapods. Activity in the dorsalis trunci of the tiger salamander (Ambystoma tigrinum) occurs contralateral to hindlimb support (Frolich and Biewener, 1992). Conversely, activity in the epaxial muscles of lizards is ipsilateral to hindlimb support (Ritter, 1995; Ritter, 1996), as is the primary burst of activity reported here for dogs. Consequently, these data provide evidence for a scenario of the evolution of epaxial muscle activity patterns that starts with uniphasic activity contralateral to hindlimb support (salamanders), with a major change in amniotes to activity ipsilateral to hindlimb support (lizards). This gross change in the activity of the epaxial muscles, coincident with the anatomical diversification of the epaxial muscles, appears to be associated with a change in the locomotor function of the epaxial muscles from lateral bending of the trunk in salamanders to stabilization of the trunk in lizards (Ritter, 1995; Ritter, 1996). Another major change from the ancestral pattern which appears to distinguish mammals is the addition of a second burst of activity during contralateral hindlimb support. This gross change in axial muscle activity pattern is associated with significant anatomical changes such as a more parasagittal limb posture and increased dorso-ventral flexibility of the body axis. Additional data addressing the diversity of axial muscle activity patterns in a wider range of cursorial species, as well as in species representing a more basal configuration, will be required to better understand the links between the anatomical, physiological and functional components of this locomotor complex.
![]() |
Acknowledgments |
---|
![]() |
References |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Banzett, R. B., Nations, C. S., Wang, N., Butler, J. P. and Lehr, J. L. (1992). Mechanical independence of wingbeat and breathing in starlings. Resp. Physiol. 89, 2736.[Medline]
Betts, B., Smith, J. L., Edgerton, V. R. and Collatos, T. C. (1976). Telemetered EMG of fast and slow muscles of the cat. Brain Res. 117, 529533.[Medline]
Carlson, H., Halbertsma, J. and Zomlefer, M. (1979). Control of the trunk during walking in the cat. Acta Physiol. Scand. 105, 251253.[Medline]
Carlson, H., Thorstensson, A. and Nilsson, J. (1988). Lumbar back muscle activity during locomotion: effects of voluntary modifications of normal trunk movements, Acta Physiol. Scand. 133, 343353.[Medline]
Carrier, D. R. (1996). Function of the intercostal muscles in trotting dogs: ventilation or locomotion? J. Exp. Biol. 199, 14551465.
English, A. W. (1980). The functions of the lumbar spine during stepping in the cat. J. Morph. 165, 5566.
Evans, H. E. (1993). Millers Anatomy of the Dog. 1113 pp. Philadelphia: Saunders.
Frolich, L. M. and Biewener, A. A. (1992). Kinematic and electromyographic analysis of the functional role of the body axis during terrestrial and aquatic locomotion in the salamander Ambystoma tigrinum. J. Exp. Biol. 162, 107130.
Gans, C. and Gorniak, G. C. (1980). Electromyograms are repeatable: precautions and limitations. Science 210, 795797.[Medline]
Gray, J. (1968). Animal Locomotion. 479 pp. New York: Norton.
Hildebrand, M. (1961). Further studies on locomotion of the Cheetah. J. Mammal. 42, 8491.
Hildebrand, M. (1995). Analysis of Vertebrate Structure. New York: John Wiley and Sons, Inc.
Loeb, G. E. and Gans, C. (1986). Electromyography for Experimentalists. Chicago and London: University of Chicago Press.
Rewcastle, S. C. (1981). Stance and gait in tetrapods: an evolutionary scenario. Symp. Zool. Soc. Lond. 48, 239267.
Ritter, D. (1995). Epaxial muscle function during locomotion in a lizard (Varanus salvator), and the proposal of a key innovation in the vertebrate axial musculoskeletal system. J. Exp. Biol. 198, 24772490.
Ritter, D. (1996). Axial muscle function during lizard locomotion. J. Exp. Biol. 199, 24992510.
Rockwell, H., Evans, F. G. and Pheasant, H. C. (1938). The comparative morphology of the vertebrate spinal column. Its form as related to function J. Morph. 63, 86117.
Shapiro, L. J. and Jungers, W. L. (1988). Back muscle function during bipedal walking in chimpanzee and gibbon: implications for the evolution of human locomotion. Am. J. Phys. Anthropol. 77, 201212.[Medline]
Shapiro, L. J. and Jungers, W. L. (1994). Electromyography of back muscles during quadrupedal and bipedal walking in primates. Am. J. Phys. Anthropol. 93, 491504.[Medline]
Thorstensson, A., Carlson, H., Zomlefer, M. R. and Nilsson, J. (1982). Lumbar back muscle activity in relation to trunk movements during locomotion in man. Acta Physiol. Scand. 116, 1320.[Medline]
Tokuriki, M. (1973a). Electromyographic and joint-mechanical studies in quadrupedal locomotion. I. Walk. Jap. J. vet. Sci. 35, 433466.
Tokuriki, M. (1973b). Electromyographic and joint-mechanical studies in quadrupedal locomotion. II. Trot. Jap. J. Vet. Sci. 35, 525533.
Tokuriki, M. (1974). Electromyographic and joint-mechanical studies in quadrupedal locomotion. III. Gallop. Jap. J. Vet. Sci. 36, 121132.
Waters, R. L., and Morris, J. M. (1972). Electrical activity of muscles of the trunk during walking. J. Anat. 111, 191199.[Medline]