The role of angiotensin II in regulating catecholamine secretion during hypoxia in rainbow trout Oncorhynchus mykiss
Department of Biology, University of Ottawa, 30 Marie Curie, Ottawa, Ontario, Canada K1N 6N5
*Author for correspondence (e-mail: sfperry{at}science.uottawa.ca)
Accepted September 20, 2001
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Summary |
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Key words: desensitisation, rainbow trout, Oncorhynchus mykiss, chromaffin cell, adrenaline, noradrenaline, hypoxia, catecholamine, nicotinic receptor, angiotensin II, serotonin, muscarinic receptor.
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Introduction |
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The control of catecholamine secretion in teleosts is achieved through several cholinergic and non-cholinergic mechanisms (Reid et al., 1998). However, it has generally been accepted that the primary mechanism initiating catecholamine release in trout involves increased neuronal stimulation by preganglionic sympathetic nerve fibres that innervate chromaffin cell cholinergic receptors (Nilsson et al., 1976
; Montpetit and Perry, 1999
; Reid et al., 1998
). The subsequent release of the neurotransmitter acetylcholine predominantly stimulates nicotinic receptors to elicit a series of Ca2+-dependent events leading to catecholamine secretion (Nilsson et al., 1976
; Montpetit and Perry, 1999
; Furimsky et al., 1996
). Recently, however, it has been demonstrated that catecholamine secretion into the circulation still occurred in hypoxic rainbow trout that possessed non-functional (desensitised) nicotinic receptors (Lapner et al., 2000
). Thus, other mechanisms are clearly contributing to catecholamine secretion at times when nicotinic receptors are non-functional.
With this background, the goal of the present study was to identify the mechanism(s) promoting catecholamine release in rainbow trout subjected to acute hypoxia under conditions of nicotinic receptor desensitisation. Three mechanisms were evaluated on the basis of previous studies demonstrating their potential involvement in catecholamine secretion in trout. First, the contribution of muscarinic receptors was assessed because it has recently been shown that muscarinic cholinergic stimulation enhances nicotinic-evoked catecholamine secretion and may, under intense stimulation, cause direct secretion (Julio et al., 1998; Montpetit and Perry, 1999
). Second, the involvement of serotonin, a secretagogue of catecholamine release in trout (Fritsche et al., 1993
), was assessed because of the known localisation of serotonergic cells in the vicinity of chromaffin tissue (Fritsche et al., 1993
; Reid et al., 1995
) and the sensitivity of similar gill serotonergic cells to oxygen (Dunel-Erb et al., 1982
). Finally, the role of angiotensin II (Ang II), the biologically active product of the reninangiotensin system (RAS), was investigated. Ang II is known to cause catecholamine release in rainbow trout (Bernier and Perry, 1997
). Further, it was recently demonstrated that hypoxia is a powerful stimulant of renin secretion and renin gene expression in rats (Ritthaler et al., 1997
). If a similar mechanism were operative in rainbow trout, Ang II could play an important role in eliciting catecholamine secretion during acute hypoxia.
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Materials and methods |
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Surgical procedures
Rainbow trout were anaesthetised in a solution of ethyl-p-amino-benzoate (benzocaine; Sigma; final concentration 2.4x104 mol l1) and placed onto an operating table where the gills were continuously irrigated with aerated anaesthetic solution. An indwelling polyethylene cannula (Clay-Adams PE50 polyethylene tubing; internal diameter 0.580 mm, outer diameter 0.965 mm) was implanted into the dorsal aorta (Soivio et al., 1975) to permit injections and periodic blood sampling. A second cannula (Clay-Adams PE160 polyethylene tubing; internal diameter 1.14 mm, outer diameter 1.57 mm) was inserted through the snout into the oral cavity to permit monitoring of inspired water PO2. To allow nicotine or saline infusion, a third cannula (PE50) was inserted into the caudal vein at the level of the caudal peduncle in the anterograde direction using standard surgical procedures (Axelsson and Fritsche, 1994
). The caudal incision was sutured using a running stitch, and the cannula was secured to the body wall with silk ligatures.
After surgery, trout were placed into individual opaque Perspex boxes supplied with aerated flowing water and allowed to recover for 24 h prior to experimentation.
Experimental protocol
To desensitise nicotinic receptors, fish were infused (0.2 ml min1) via the caudal vein with nicotine (1.3x105 mol kg1 h1) using a syringe infusion pump (Sage Instruments) for 60 min (Lapner et al., 2000); control fish were infused with Cortland saline (Wolf, 1963
). During continuing infusion, the fish were subjected to 10 min of hypoxia (beginning when the inspired PO2 had fallen to 4045 mmHg; 5.36.0 kPa).
Acute hypoxia was achieved by replacing the air supplying a water/gas equilibration column with N2. The desired water PO2 (PwO2) (4045 mmHg) was pre-set and established by adjusting the rate of water and/or N2 flow through the column. This level of hypoxia was chosen on the basis of a previous study (Perry and Reid, 1992) that demonstrated significant catecholamine release in rainbow trout using this protocol. The PwO2 within the experimental box was monitored continuously using a peristaltic pump (flow rate 0.6 ml min1) that withdrew water from each individual trout box and passed it across a PO2 electrode (Cameron Instruments) connected to an O2 meter (Cameron Instruments). Generally, the desired PwO2 in the experimental box was reached within 10 min and thereafter never varied more than ±5 mmHg (0.67 kPa). After experimentation, PwO2 was restored to normoxic levels and the fish were allowed to recover.
The involvement of muscarinic receptors, serotonin or the RAS in promoting catecholamine release
After 40 min of infusion (nicotine or saline) under normoxic conditions, fish were injected via the dorsal aorta with the muscarinic receptor antagonist atropine (1 µmol kg1). Efficacy of blockade was confirmed in preliminary experiments by comparing the cardiac frequency responses of control and atropinised fish to the muscarinic receptor agonist methacholine. The infusion continued for a further 20 min prior to the commencement of hypoxia (see above). Using the same protocol, a separate group of fish was injected with the serotonin receptor antagonist methysergide (108 mol kg1) prior to hypoxia. Efficacy of blockade was not evaluated in the present study, although a previous study (Fritsche et al., 1992) demonstrated that the same dose of methysergide was effective at blocking serotonergic receptors in rainbow trout. Finally, a third group was treated (as above) with the Ang-II-converting enzyme (ACE) inhibitor lisinopril (104 mol kg1) to prevent the formation of Ang II (Bernier et al., 1999b
). The effectiveness of ACE inhibition was established in preliminary experiments in which blood pressure changes were measured after injection of Ang I. Control fish were injected with saline after 40 min of infusion.
Determination of plasma catecholamine and Ang II levels
Blood samples collected for catecholamine analysis were taken immediately prior to beginning the infusion and after 10 and 60 min of infusion. Prior to hypoxia, samples withdrawn for Ang II analysis were only taken at 60 min of infusion. For measurement of plasma catecholamine and Ang II levels, four blood samples were withdrawn during the hypoxic period: at 0, 3, 5 and 10 min after reaching the targeted 4045 mmHg PwO2. Because of fluctuating PwO2 levels during the 10 min hypoxic period (±5 mmHg; 0.67 kPa), the blood sample that was withdrawn at a PwO2 level nearest to the target of 40 mmHg was used to represent levels during hypoxia. The collected blood samples were placed into micro-centrifuge tubes (1.5 ml) and centrifuged (12 000 g for 20 s). The plasma was transferred to micro-centrifuge tubes containing 10 µl (25 units) of heparin (ammonium salt). Samples were quick-frozen in liquid N2 and then stored at 80°C until subsequent analysis.
Extraction of plasma catecholamine samples
All plasma catecholamine samples were subjected to alumina extraction and then analysed by high-pressure liquid chromatography (HPLC) with electrochemical detection (Woodward, 1982). 3,4-Dihydroxybenzylamine hydrobromide was used as an internal standard in these analyses. Detection limits for adrenaline and noradrenaline were 0.1 nmol l1.
Determination of angiotensin II levels by radioimmunoassay
Plasma samples were extracted according to the method of Phillips et al. (1991), with incorporated modifications by Bernier et al. (1999a
) and by us. Just-thawed plasma (0.1 ml) was mixed with acidic acetone (volume ratio of acetone:H2O:1 mol l1 HCl=40:5:1) and vortexed vigorously for 10 s. The mixture was centrifuged for 10 min at 10 000 g and 4°C. The supernatant was collected in a new tube, and the pellet was re-solubilised and re-extracted with 0.1 ml of acidic acetone. Once combined, the supernatants were centrifuged for 10 min as above. The supernatant was collected into a new tube and lyophilized. For the radioimmunoassay (RIA), the extracted pellet was re-suspended in 0.025 ml of RIA buffer (diluent). The recovery rate of Ang II through this extraction procedure, as measured with 125I-labelled [Asn1,Val5]-Ang II, was 89.8 %.
A single-antibody method was used that employed an Ang II antibody designed for the detection of mammalian Ang II ([Asp1,Ile5]-Ang II). Recently, however, it has been shown that a similar mammalian antibody cross-reacts (approximately 70 %) with rainbow trout Ang II ([Asn1,Val5]-Ang II) and does not cross-react with piscine or mammalian Ang I (Bernier et al., 1999a).
96-well microtitre plates (Wallac; Rigid Plates) were prepared by coating each well with 100 µl of Protein A/G (1 µg ml1 prepared in 0.1 mol l1 NaHCO3; pH 9.0). Plates were wrapped in Parafilm and stored at 4°C overnight. The following day, the plates were washed twice with wash buffer [0.1 % Tween-20 in diluent (0.5 % bovine serum albumin, BSA, in 0.08 mol l1 barbitol buffer)] for 2 min each at room temperature (20°C). The plates were then washed once with diluent for 20 min at room temperature and were blotted dry on paper towel for 1 min. Antibody (1:200 000 dilution of rabbit anti-Ang-II serum; Amersham Pharmacia Biotech) or normal rabbit serum (to determine non-specific binding) was added to the appropriate wells. The plates were wrapped in Parafilm and incubated at 4°C overnight. The following day, the plates were washed with wash buffer three times for 2 min each at room temperature. The plates were blotted dry on paper towel for 1 min before adding 50 µl of RIA buffer (diluent) to each well. Radiolabelled hormone {25 µl of 125I-labelled Ang II ([Asp1,Ile5]-Ang II; Amersham Pharmacia Biotech; reconstituted to 100 µCi ml1 with water, and diluted to a final concentration of 400 disints min1 l1 with diluent)} and 25 µl of standard (unlabelled [Asn1,Val5]-Ang II) or sample was added to the appropriate wells. Where required, diluent was added to cells such that each well contained a total volume of 100 µl. The plates were wrapped in Parafilm and incubated at 4°C for 48 h with intermittent gentle shaking after 24 h. After the 48 h incubation period, the plates were washed three times with wash buffer for 2 min each at room temperature. The plates were blotted dry on paper towel before adding 100 µl of scintillation cocktail to each well. Labelled hormone (25 µl) was added to the appropriate wells for determination of total radioactivity. The plates were incubated at room temperature for 2 h before counting in a MicroBeta Liquid Scintillation and Luminescence Counter, using a MicroBeta Windows Workstation. Data were analysed in MS-DOS using a WIA Level 5.M MultiCalc Advanced program.
Statistical analyses
The data are presented as means ±1 standard error of the mean (S.E.M.). Where appropriate, data were analysed statistically using one-way analysis of variance (ANOVA) followed by Dunns multiple-comparison test. If assumptions for parametric statistics were violated, an ANOVA on ranks was performed followed by Dunns multiple-comparison test. In other instances, data were analysed using Students t-tests and, if assumptions for parametric statistics were violated, a MannWhitney rank sum test was performed. All statistical analyses were performed using commercial software (SigmaStat Version 2.0; SPSS). The fiducial limits of significance were set at 5 %.
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Results |
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Mechanisms eliciting catecholamine release during nicotinic receptor desensitisation
All fish displayed the characteristic transient increase in plasma catecholamine levels at 10 min of nicotine infusion that is indicative of nicotinic receptor desensitisation (Fig. 2). Blockade of muscarinic (Fig. 2A) or serotonergic (Fig. 2B) receptors did not diminish plasma catecholamine levels during acute hypoxia (compare with Fig. 1A). However, blockade of the RAS abolished the ability of desensitised fish to mobilize circulating catecholamines during hypoxia (Fig. 2C). Fig. 3 effectively illustrates the importance of the RAS in promoting catecholamine secretion during hypoxia in desensitised fish.
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Discussion |
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Catecholamine release in desensitised fish
Upon stimulation, the nicotinic receptor of trout chromaffin cells, as in mammals (Boksa and Livett, 1984), undergoes rapid desensitisation (Lapner et al., 2000
). The re-sensitisation process is gradual (e.g. as long as 40 min in rainbow trout) (Lapner et al., 2000
). Thus, although it is believed to be a mechanism preventing excessive catecholamine secretion, nicotinic receptor desensitisation, if prolonged, could potentially impair catecholamine release in animals experiencing repeated bouts of acute stress. The single previous study to address this issue, however, demonstrated that the ability of trout to release catecholamines during acute hypoxia was not impaired during periods of nicotinic receptor desensitisation (Lapner et al., 2000
). This finding indicated that other pathways that are not reliant on nicotinic receptors were activated to elicit catecholamine secretion. The goal of this study was to attempt to identify one or more of these pathways. It was necessary, however, first to confirm that catecholamine release during hypoxia was indeed unimpaired in trout experiencing nicotinic receptor desensitisation. Thus, using a protocol of intravascular nicotine infusion to desensitise nicotinic receptors (Lapner et al., 2000
), initial experiments revealed similar elevations of plasma catecholamine levels during hypoxia irrespective of the state of the nicotinic receptor. Because local hypoxia in the vicinity of chromaffin cells does not evoke catecholamine secretion in rainbow trout (Perry et al., 2000
), subsequent experiments were designed to establish alternative mechanisms of catecholamine release in desensitised fish.
Potential involvement of serotonin
Although serotonergic receptors are present on rainbow trout chromaffin cells and the ability of serotonin to elicit catecholamine secretion has previously been demonstrated in vivo and in situ (Fritsche et al., 1993), serotonin does not appear to be a secondary catecholamine secretagogue during hypoxia when nicotinic receptors are desensitised. Previous work (Fritsche et al., 1993
) showed that injection of the serotonergic receptor antagonist methysergide blocked serotonin-induced adrenaline release in situ but not in vivo. It was suggested that this discrepancy could be attributed to serotonin acting on methysergide-insensitive receptors within higher control centres in vivo. Furthermore, Perry et al. (2000
) showed that localised hypoxia inhibited chromaffin cell responsiveness to nicotine in situ but had an opposite, enhancing effect in vivo, further supporting the idea that higher control centres are involved in catecholamine release in vivo. Therefore, although our results cannot rule out the possibility that serotonin is acting indirectly on higher control centres to elicit catecholamine secretion during nicotinic receptor desensitisation, we can exclude any direct effect of serotonin on chromaffin cells.
The possible involvement of muscarinic receptors
Previous in situ studies have revealed that activation of chromaffin cell muscarinic receptors can enhance nicotine-evoked catecholamine secretion, and it has been suggested that, under intense stimulation, muscarinic receptors might directly cause catecholamine secretion (Montpetit and Perry, 1999). The results of the present study, however, demonstrate that muscarinic receptors do not contribute to catecholamine release during hypoxia in nicotinic receptor-desensitised fish. In contrast, stimulation of chromaffin cell muscarinic receptors in mammals can evoke significant catecholamine secretion during periods of nicotinic receptor desensitisation (Malhotra et al., 1989
). In trout, it seems that chromaffin cell muscarinic receptors serve only to enhance the nicotinic-evoked catecholamine secretion (Montpetit and Perry, 1999
) but are incapable of independently eliciting catecholamine release during stress (at least acute hypoxia).
Potential involvement of the reninangiotensin system
Although chromaffin cell serotonergic and muscarinic receptors do not appear to be involved in eliciting catecholamine secretion during hypoxia in trout experiencing nicotinic receptor desensitisation, the results of the present study indicate an essential role of the RAS. Under normal conditions, the RAS is an important regulator of cardiovascular function in fish (Olson, 1992). Recently, a link between the RAS and circulating catecholamine levels has been established in cardiovascular control whereby Ang II, mobilised during hypotension, dose-dependently stimulates catecholamine release (Bernier and Perry, 1997
; Bernier et al., 1999b
). Thus, typically, Ang II and catecholamines function together as vasopressive agents to regulate blood pressure during periods of hypotension (Nishimura, 1985
; Oudit and Butler, 1995
; Fuentes and Eddy, 1998
; Bernier and Perry, 1999
). The present paper provides the first evidence of RAS recruitment during acute hypoxia, a condition that is not associated with hypotension. Further, the results demonstrate that activation of the RAS is a prerequisite for catecholamine secretion in hypoxic trout possessing desensitised nicotinic receptors.
Circulating Ang II is derived via activation of systemic and/or regional reninangiotensin systems (Bernier and Perry, 1997). In mammals, key components indicative of a local RAS have been demonstrated in multiple tissues of rodents (Campbell and Habener, 1986
; Dzau et al., 1987
; Leung et al., 1999
). Interestingly, secretory granules in adrenaline-containing chromaffin cells of rat adrenal medulla contain both renin and pro-renin (Berka et al., 1996
). Furthermore, chronic or acute hypoxia has recently been shown to result in the enhanced expression of local RAS component genes in rat pancreas (Chan et al., 2000
) and primary cultures of renal juxtaglomerular cells (Ritthaler et al., 1997
), to increase the numbers of lung Ang II receptors (Zhao et al., 1996
) and to increase cardiac ACE activity (Morrell et al., 1997
). In rat renal juxtaglomerular cells, renin secretion and renin gene expression were indirectly stimulated by acute hypoxia; their expression was enhanced in vivo but not in situ. The origin of the increased circulating Ang II levels was not investigated in the present study. Further experiments are required to determine whether, as in mammals, local or systemic RAS components exhibit increased expression during hypoxia.
Angiotensin II levels were elevated equally during hypoxia in control fish and in fish experiencing nicotinic receptor desensitisation. However, unlike in the desensitised fish, blockade of the RAS did not prevent catecholamine release in control fish exposed to hypoxia. This result reinforces the idea that stimulation of nicotinic receptors via activation of sympathetic nerve fibres is the dominant pathway controlling catecholamine secretion in trout under normal conditions. Angiotensin II, however, may be contributing to the overall response in control fish on the basis of the significant reduction in total plasma catecholamine levels after RAS blockade. Previous studies have demonstrated that Ang II preferentially stimulates adrenaline secretion from fish chromaffin cells (Bernier and Perry, 1997). Interestingly, however, RAS blockade prevented the release of both catecholamines in desensitised fish and had a greater effect on noradrenaline secretion in control fish.
Most teleosts that have been studied, including trout, exhibit pronounced cardiovascular adjustments when exposed to hypoxia including bradycardia and hypertension (for a review, see Fritsche and Nilsson, 1993). Traditionally, the hypertension has been attributed to increased systemic vascular resistance owing to increased activity of sympathetic nerve fibres as well as elevated circulating catecholamine levels. In the light of the results of the present study showing elevated Ang II levels in hypoxic trout, it seems that additional mechanisms may be contributing to the hypoxic hypertension, including direct vasoconstrictory effects of Ang II and indirect effects of Ang II in evoking catecholamine secretion.
As in mammals, stimulation of the preganglionic nerve fibres that innervate the chromaffin cells leads to the release of both cholinergic (i.e. acetylcholine) and non-cholinergic neurotransmitters (Montpetit and Perry, 2000). In particular, vasoactive intestinal polypeptide (VIP) and pituitary adenylyl-cyclase-activating peptide (PACAP) are potent catecholamine secretagogues in rainbow trout (Montpetit and Perry, 2000
). Because blockade of the RAS abolished catecholamine secretion during hypoxia in fish with desensitised nicotinic receptors, it would appear that these non-cholinergic neurotransmitters were not secreted in sufficient quantities to evoke catecholamine release. The relative rates of secretion of cholinergic versus non-cholinergic neurotransmitters are partially dependent on the frequency of neuronal action potentials. Specifically, in trout, the secretion of non-cholinergic neurotransmitters is favoured under conditions of low-frequency nerve activity (Montpetit and Perry, 2000
), a situation that might not exist during acute hypoxic stress. Thus, while catecholamine release in trout is thought to be controlled by multiple redundant pathways (see Reid et al., 1998
), the specific involvement of each of these pathways may depend on the precise nature of the stressor. For example, while it is clear that serotonin, VIP, PACAP and muscarinic receptor stimulation are capable of independently eliciting catecholamine secretion (Reid et al., 1998
), they appear to play no role during hypoxia. Thus, during hypoxic stress, they cannot complement or replace the nicotinic-receptor-mediated pathway of catecholamine secretion when nicotinic receptors are desensitised. Their possible role during other types of stress, however, cannot be ruled out. Ang II, usually considered to be a catecholamine secretagogue during periods of hypotension (Bernier et al., 1999a
,b
), also contributes to catecholamine release during hypoxia and is indeed essential to allow catecholamine release in fish experiencing nicotinic receptor desensitisation.
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Acknowledgments |
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