Effects of aging on behavior and leg kinematics during locomotion in two species of cockroach
Department of Biology, Case Western Reserve University, Cleveland, OH 44106, USA
* Author for correspondence (e-mail: alr17{at}cwru.edu)
Accepted 8 September 2003
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Summary |
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Key words: senescence, walking, arthropod, central nervous system, kinematics
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Introduction |
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Age-related locomotor deficits are often the result of degeneration in the
central and/or peripheral nervous systems. In mammals, loss of neurons and
neurotransmitters in cortex and cerebellum can be associated with aging and
motor impairment (Hilber and Caston,
2001; Kaasinen et al.,
2000
; Volkow et al.,
1998
). Sensory information from the limbs is important in postural
control and reactions to perturbations
(Maki and McIlroy, 1996
;
McIlroy and Maki, 1996
).
Degeneration in the peripheral nervous system could result in loss of
vibration or cutaneous sense in the limbs and a decrease in the response time
of reflexes (Shumway-Cook and Woollacott,
2000
; Ulfhake et al.,
2002
).
Locomotor deficits in aged individuals can also be caused by non-neural
degeneration in the musculoskeletal system. Aging is frequently associated
with weakness and decreased mass in the muscles
(Anderson, 2003). Muscle
weakness in the elderly has been attributed to a reduction in the sliding
speed of cross-bridging elements (Hook et
al., 2001
; Lowe et al.,
2002
). Muscle atrophy with aging is the result of a loss in the
number of muscle fibers, rather than a reduction in fiber size
(Lexell, 1993
). Stiffness at
the joints and ligaments, which can be caused by increases in collagen fiber
cross-linkages and loss of elastic fibers, can also result in a decrease in
the range of motion in aged individuals
(Aigner and McKenna, 2002
;
Kerin et al., 2002
).
Insects provide a useful model system for aging studies because they are
short-lived compared with mammals. Decreases in spontaneous activity with
increasing age have been well documented in fruitflies and honeybees
(Fernandez et al., 1999;
Minois et al., 2001
;
Parkes et al., 1999
;
Tofilski, 2000
). However, none
of these papers have examined changes in leg kinematics and joint movement in
aged insects. Furthermore, past studies have only looked at simple walking and
have not studied more complex locomotion, such as `transitional behaviors'
that alter an animal's path of movement
(Watson et al., 2002
).
Transitional behaviors often require altered leg movements and/or posture to
complete the task. Examination of these types of locomotion is important in
order to understand how an animal alters behavior in different environmental
situations.
In the present study, we documented the adult lifespan of Blaberus discoidalis and Periplaneta americana cockroaches. We then examined behavioral and kinematic changes in locomotion with age in Blaberus during horizontal walking and transitional behaviors including righting, climbing and inclined walking. In addition, we studied the effect of advanced age on the kinematics of escape behavior in Periplaneta and compared these findings with those observed in young individuals and decapitated animals. Locomotor deficits were present in both species of cockroaches older than 60 weeks post-adult molt and the behavior of these animals was often different from that seen in younger animals. Our findings show that age-related deficits in locomotion are present in senescent cockroaches.
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Materials and methods |
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Spontaneous locomotion
Animals were placed into a 23 cmx30 cmx10 cm plastic container
without any shelter. Spontaneous locomotion was defined as the amount of time
an individual spent walking, tunneling or climbing the walls within a 10 min
period (600 s). Tunneling was defined as the time in which an animal was
moving the legs in a walking motion but the head was in contact with the wall.
Climbing was defined as the time in which one or more of the legs were in
contact with the wall of the arena and the legs were moving in a walking
motion. Grooming and standing were not scored as locomotion. In order to
maintain the novelty of the testing arena, animals were tested only once in
each trial.
Horizontal walking
Animals were placed in a treadmill that allowed for observation from the
side and from below via a mirror mounted at 45°
(Watson and Ritzmann, 1998).
High-speed video images (125 frames s-1) were taken with a single
camera. The position of the tarsus, the end of the tibia on each leg and the
center of mass (COM) were digitized using motion analysis software
(WINanalyse; Mikromak, Berlin, Germany). The COM was estimated as a point 46%
of the body length along the fore-aft axis from the tail point of the animal
(Jindrich and Full, 1999
;
Kram et al., 1997
). Digitized
points were used to calculate the distance from the end of the tibia to the
COM (r) and the angle of the end of the tibia relative to the
fore-aft axis through the COM (
; terminology from
Jindrich and Full, 1999
). The
angle of the tibio-tarsal joint was calculated during the first frame of
stance by digitizing the end of the tarsus and a point on the distal end of
the tibia. Angles and distances were linearly smoothed with an 8 ms time
constant using Data-Pac software (Run Technologies, Mission Viego, CA, USA)
and plotted as polar coordinates (r,
). Stride length was
calculated as the length of the distance between the anterior extreme position
(AEP) and posterior extreme position (PEP) during each stance phase. Gait
pattern and the speed of walking were determined by calculating the number of
frames in which the legs were in stance and swing.
Tarsal anatomy studies
The external and internal morphology of the tarsi was examined under a
dissecting microscope, and images were captured with a Nikon Coolpix 950
digital camera. To test the flexibility of the tarsal pads, the tarsus was
secured, tarsal pads up, in a wax dish with insect pin staples so that it
would not move. A single nylon filament attached to a glass rod and a
micromanipulator was used to indent the most proximal pad until the filament
buckled. The maximum force of the filament (29 mN) was determined by pushing
it against a 10 g force transducer until it buckled. The force transducer was
calibrated with small weights.
Block climbing, inclined walking and righting
To examine climbing in aging animals, acrylic blocks were placed across the
path of the treadmill (Watson et al.,
2002). Blocks of 5.5 mm, 11 mm and 16.5 mm height were used to
test each individual. High-speed video images were taken with a single camera
at 125 frames s-1. Videos were examined and compared with previous
studies (Watson et al.,
2002
).
The ability of aged individuals to successfully walk on an incline was tested by using a track with an acetate belt that was tilted to 45°. Two high-speed video cameras were used to visualize the animal from below and from the side. Foot placement was calculated as described above. Leg slipping was defined as a change in the position of the foot on the substrate during the stance phase and was tallied in all six legs in four consecutive steps. Within each animal, the probability of leg slipping was calculated as the number of slips per step in all trials. Probability values for slipping for each individual were used to calculate the mean and S.D. across the population.
Righting was examined by placing animals on their backs in a 23 cmx30 cmx10 cm plastic container that was lined with a piece of Styrofoam. The duration of righting was measured with a stopwatch. Each individual was tested four times and trials were averaged.
Escape behavior
To examine the effect of aging on escape behavior, we switched our focus to
Periplaneta americana. The reason for this change is that most of the
previous work, by far, on cockroach escape has been performed on
Periplaneta (Camhi,
1988; Ritzmann,
1993
; Ritzmann and Eaton,
1997
). Indeed, in an earlier study
(Simpson et al., 1986
), a
related blaberid species, Blaberus craniifer, failed to escape from a
live predator or move away from a synthetically generated puff of wind at room
temperature. Although B. discoidalis does make escape movements, they
are not as vigorous as those of Periplaneta.
For escape studies, animals were tethered above a glass plate covered with
microtome oil (Nye and Ritzmann,
1992; Tryba and Ritzmann,
2000
). Two large pins were attached to a glass tube mounted on a
manipulator and placed through the pronotum from the ventral surface on either
side of the head. Animals were induced to escape using tactile stimulation, in
a random order, to the left and right edges of the 3rd abdominal tergite
(Schaefer and Ritzmann, 2001
).
Stimulation was delivered with a hand-held solenoid that moved a glass rod
with a bent insect pin at the end. The stimulator was held approximately 1 mm
above the cuticle, and activation of the solenoid produced a stimulus with a
force of 0.18 N. Each individual was stimulated four times, and then the
animals were decapitated and stimulated several more times. Escapes were
recorded with a Redlake Motionscope camera at 250 frames s-1. Leg
joint angles were analyzed with Videoblaster card and Motion TV motion
analysis software (DataCrunch Systems, San Clemente, CA, USA).
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Results |
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In adult Blaberus cockroaches, we measured a gradual, but significant, decrease in spontaneous locomotion with increasing age (regression analysis, slope=-3.4, r2=0.839, P<0.01; Fig. 1B). Aged animals walked more slowly than young individuals and spent more time grooming or standing still. In addition, they showed less climbing and tunneling behavior than young adults. Young Periplaneta readily showed walking movements on a tether, but old individuals showed little spontaneous movement while tethered.
Horizontal walking
To characterize locomotor deficits in aged Blaberus, we examined
the kinematics of walking on a horizontal surface (N=21 animals; age
range, 60-71 weeks post-adult molt). A number of differences in behavior
between young and aged adults were measured. First, the mean speed of walking
in 1-week-old animals was 4.6±1.7 steps s-1 (N=6
animals, 26 trials). By contrast, walking speed was significantly slower
(2.7±0.7 steps s-1) in 60-week-old adults (independent
t-test, P<0.05, N=7 animals, 13 trials). Second,
an unexpected pathology, which we termed `tarsus catch', was detected in many
of the aged animals. In these individuals, the tarsus of the prothoracic leg
regularly catches on the spines of the femur or at the femur-tibia joint on a
metathoracic leg (Fig. 2A; see
Movie). `Tarsus catch' was detectable in 90% of the animals observed at 65
weeks post-adult molt (Fig.
2B). The percentage of animals that showed `tarsus catch'
decreased after 65 weeks because the majority of individuals showing this
pathology died shortly after that time.
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'Tarsus catch' regularly results in an increase in the length of the swing
phase in the prothoracic leg and a subsequent alteration of the gait pattern
in the other legs. Prior to tarsus catch, 73% of aged individuals
(N=8 of 11) walked with an alternating tripod gait (phase 0.5 for
all neighboring legs). Fig. 2C
shows an example of a gait pattern in which the right prothoracic tarsus
catches on the right mesothoracic leg (shown by vertical line). While the
prothoracic tarsus is caught on the mesothoracic leg, the tripod gait is
disrupted, because the left front and left rear legs fail to swing (shown by
circle). However, the alternating tripod gait quickly returns after the front
leg tarsus is released. In the majority of individuals (N=10 of 11),
only a single prothoracic leg showed `tarsus catch'. In these trials, the
right and left prothoracic tarsi were affected equally (50% left leg, 50%
right leg). However, one individual exhibited `tarsus catch' in both
prothoracic legs.
'Tarsus catch' was predominantly found only in prothoracic legs. Although it may be physically impossible for the metathoracic tarsi to catch on anterior legs, mesothoracic tarsi can catch. However, it is a rare occurrence. In one animal, the mesothoracic tarsus caught the ipsilateral metathoracic leg. That animal also showed prothoracic catch.
We studied leg placement during walking in aged animals to determine if
`tarsus catch' is caused by an alteration of leg kinematics. Specifically, we
looked for changes in anterior extreme position (AEP) or posterior extreme
position (PEP) of the legs in aged animals before and after `tarsus catch' was
present. These values are important because they denote the beginning and end
of the swing phase of walking. Alteration in the PEP position of the front
legs or the AEP of the middle legs could contribute to tarsus catch. We
digitized the end of the tibia in all six legs and the COM
(Fig. 3A). We then calculated
the distance from the end of the tibia to the COM (r) and the angle
of the end of the tibia () relative to the fore-aft axis (represented by
a line from the head to the tail and through the COM in
Fig. 3A). We chose to digitize
the end of the tibia because the orientation of the tarsus was often altered
in aged animals (see Fig. 4).
Fig. 3B shows a polar plot of
the distance and angle values in all six legs during the stance phase of an
aged animal before `tarsus catch' developed (60 weeks post-adult molt). Each
line represents a successive step and indicates the direction of leg movement
relative to the fore-aft axis. The arrow originates at the AEP and terminates
at the PEP; the beginning of the arrow is the AEP, while the arrowhead denotes
the PEP. Fig. 3C is a polar
plot from the same animal, at week 63, after `tarsus catch' is present. Leg
placement in young adults is similar to that observed in old intact animals
(Fig. 3D).
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In an attempt to establish if changes in foot placement with aging are
consistent within a population, we compared the mean values of r and
at the AEP and PEP in the same animals (N=4 animals, 8 trials,
56-64 steps) before and after they developed `tarsus catch'
(Fig. 3E). In trials after
animals developed `tarsus catch', we only analyzed steps before `tarsus catch'
occurs. Overall, there were minor differences in foot placement among trials
(Table 1; independent
t-test, P<0.05). These changes were not consistent from
the left to the right side. The AEP
and the PEP
of all three legs
(T1, T2 and T3) on the left side significantly decreased in trials after
`tarsus catch' was present. However, only the PEP
in the metathoracic
leg was reduced. In addition, slight changes in the PEP and AEP r
were present but not consistent among legs or among segments. Differences in
the values of r and
at the AEP and PEP are subtle and result in
a slight decrease in the horizontal position of the tarsi relative to the body
(sprawl) in the front legs and an increase in the sprawl of the rear legs in
individuals with `tarsus catch'. Nevertheless, these changes are not
sufficient to cause `tarsus catch' in these animals.
While examining videos of horizontal walking, we noticed that the angle of the tibio-tarsal joint was altered in individuals with `tarsus catch' (Fig. 4). The angle of the tibio-tarsal joint was 115±15.3° in animals with `tarsus catch' (N=12 animals, 32 trials) and 155.3±20.8° in intact cockroaches (N=5 animals, 14 trials). The decrease in the angle of this joint in individuals with `tarsus catch' is highly significant (independent t-test, P<0.01). To determine the changes in the morphology of the tarsus with age, we examined the condition of the tarsal pads, trachea, tendons, nerves and muscles in young and aged animals. In young cockroaches, the tarsal pads and the tarsal joints were flexible and white in color (Fig. 5A). By contrast, in aged animals, the joints were stiff and the tarsal pads were brown in color (Fig. 5B). Furthermore, the tarsal pads in young individuals were flexible and easily deformed with a nylon filament that produced 29 mN of force (Fig. 5C). Tarsal pads in aged individuals were hardened and did not deform when compressed with the nylon filament (Fig. 5D). The internal morphology of the tarsi was consistent with the external appearance. The trachea and tendon inside the tarsus of young animals were silver and shiny (Fig. 5E), while there was hardening and degeneration of the trachea and tendon in aged cockroaches (Fig. 5F).
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Climbing
In light of the deficits seen in the horizontal walking trials, we tested
the ability of aged animals to perform transitional behaviors such as
climbing, righting and inclined walking. These types of behaviors often
require altered gaits, body posture and leg positions to complete the task
(Watson et al., 2002).
We examined block climbing (5.5 mm, 11 mm and 16.5 mm in height) in both
aged and 1-week-old adults. Young individuals were easily able to surmount all
blocks (N=6 animals, 33 trials). Although old animals often show
`tarsus catch', they were also able to successfully climb blocks
(N=16 animals, 90 trials). Generally, all animals were able to climb
a 5.5 mm block without alteration of gait or posture. Climbing strategies for
larger obstacles (11 mm and 16.5 mm) were categorized as (1) `rear up'
(Fig. 6A,B) or (2) `head butt'
(Fig. 6C) according to the
terminology of Watson et al.
(2002). The most prominent
strategy of all aged individuals (78% of trials of `tarsus catch'; 100% of
trials of `no catch') was to change the body angle before placing the front
legs on the block (Fig. 6D;
rear up). This is consistent with previous reports
(Watson et al., 2002
).
Cockroaches with `tarsus catch' sometimes climb the obstacle by pushing the
head against the block, thereby forcing the body over the obstacle (head
butt).
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There were additional differences in climbing behavior between intact aged cockroaches and those with `tarsus catch'. Some animals with `tarsus catch' appeared to use the prothoracic legs to pull the body onto the block instead of extending the metathoracic legs to lift the COM over the obstacle (28% of the trials). However, they often had trouble grasping the top of the block with the prothoracic tarsi. Animals with `tarsus catch' showed leg slipping in 85% of the trials (N=47) as compared with 18% of trials in intact aged individuals (N=28). The inability of these cockroaches to grasp the surface often results in the animals becoming `high-centered' on the corner of the block. However, in all cases, they were able to recover and continue over the obstacle.
Righting
Righting behavior in cockroaches occurs when an animal is placed on its
back and leg-to-ground contact is lost. With loss of ground contact, load
receptors on the legs would not be activated as they are in standing or
walking. In order to examine locomotory behavior under altered sensory
conditions, we recorded the duration of righting in aged (59-, 60- and
63-week-old individuals) and 1-week-old adult cockroaches. Righting in
cockroaches involves rotation about the fore-aft axis of the body while using
the rear leg as an anchoring point and often includes dorsal flexions of the
body (Camhi, 1977;
Full et al., 1995
). Aged
individuals (59 weeks, N=4 animals, 16 trials; 60 weeks, N=8
animals, 24 trials; 63 weeks, N=5 animals, 20 trials) are readily
able to right themselves as rapidly as 1-week-old adults (N=9
animals, 36 trials; Fig. 7).
However, more variability in the duration of righting is present among aged
individuals. Furthermore, there was a significant increase in the duration of
righting in old adults with `tarsus catch' (4.96±3.7 s, 60 and 63 weeks
old, N=5 animals, 20 trials) when compared with intact aged animals
(3.67±2.4 s, N=6 animals, 24 trials; independent
t-test, P<0.05). These results show that old animals are
able to right themselves, but cockroaches with `tarsus catch' are slower at
accomplishing this task.
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Inclined walking
Aged animals (N=13 individuals, 27 trials) were placed in the
arena, and trials were deemed successful if the animal moved the caudal end of
the abdomen forward from the start of the incline (Start;
Fig. 8A) to a point
approximately one body length from the start (End;
Fig. 8B). Young animals have no
difficulty in performing this task. Only 58% of aged animals were able to
climb the incline in at least one of the trials. However, the presence of
`tarsus catch' was not sufficient to determine if animals would fail in the
inclined climbing task. Inclined walking was successful in 62.5% of the
animals (N=5 of 8) that showed `tarsus catch'. However, only 40% of
individuals (N=2 of 5) without `tarsus catch' were able to climb the
incline. By contrast, all younger animals were successful (N=13
animals; A. J. Pollack, personal communication). Failure to climb the incline
in aged cockroaches may simply be due to deterioration of the tarsal pads (see
Fig. 5). `tarsus catch' may
simply represent an extreme condition. However, these findings do not rule out
the possibility that changes in the central nervous system (CNS) or muscular
system in aging may also contribute to failure in inclined walking.
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Aged animals showed extensive leg slipping while walking on the inclined treadmill. In a few animals (N=5 of 13), the caudal end of the abdomen did not reach the start point. These trials were not used in the leg slipping analysis. Differences in the probability of leg slipping between intact animals (N=3 animals, 6 trials, 24 steps) and those with `tarsus catch' (N=5 animals, 10 trials, 40 steps) were not detectable (Fig. 8C).Generally, the prothoracic legs slipped on the inclined acetate surface more often than the mesothoracic or metathoracic legs. However, there was significantly more leg slipping in the mesothoracic legs during failing trials (N=3 animals, 6 trials, 24 steps) than during successful trials (N=6 animals, 10 trials, 40 steps; P<0.05; Fig. 8D). Individuals that were able to climb the incline generally walked in a metachronal gait (Fig. 8E). By contrast, individuals that failed during inclined walking often showed uncoordinated gait patterns during the attempt (Fig. 8F). However, all aged animals that failed on the incline walked with a tripod gait on a horizontal surface.
Escape behavior
The escape behavior of Periplaneta americana is one of the most
characterized behaviors in cockroaches and in arthropods in general
(Ritzmann and Eaton, 1997;
Comer and Robertson, 2001
). The
neural circuit underlying this behavior has been described in detail. Most of
the neural control resides in the thoracic and abdominal ganglia. However, a
descending influence has recently been demonstrated
(Schaefer and Ritzmann, 2001
).
Given this background information, we decided to compare escape responses in
young and aged cockroaches and to examine whether deficits were attributed to
the basic thoracic circuitry or to the influences found in the higher centers
(brain and subesophageal ganglia).
Tactile stimulation of the dorsal abdominal cuticle readily evokes escape
responses in tethered American cockroaches
(Schaefer and Ritzmann, 2001;
Schaefer et al., 1994
).
However, similar stimulation of aged animals (61 weeks post-adult molt) failed
to evoke any escape responses (Fig.
9; N=3 animals, 12 trials). Interestingly, the capacity
of aged animals to escape returned after decapitation, suggesting that the
deficit associated with aging was, in fact, primarily focused within the head
ganglia. After decapitation of aged cockroaches (N=3 animals, 22
trials), escape behavior was elicited in 72.7% of the trials. Of the evoked
escapes post-decapitation, 25% were non-directional and one involved what
appeared to be a turn towards the direction of the stimulus. Consistent with
previous observations on decapitated cockroaches
(Schaefer and Ritzmann, 2001
),
no subsequent runs were observed following escape behavior in decapitated
individuals. However, uncoordinated and erratic leg movements occurred after
the escape in two trials. To examine leg kinematics during escape behavior in
aged animals, we measured joint angle excursions in the metathoracic and
mesothoracic legs of decapitated 61-week-old cockroaches. Robust movements of
the metathoracic legs were observed, with smaller excursions for the
mesothoracic legs. These findings are similar to that observed in younger
decapitated animals (Schaefer and
Ritzmann, 2001
). These data show that escape behaviors are
disrupted in aged cockroaches and suggest that these deficits may be caused by
age-related degeneration in the brain or subesophageal ganglia.
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Discussion |
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We have shown that aged cockroaches have reduced activity levels and often
have difficulty in complex locomotor tasks. Many of these animals also develop
`tarsus catch' in the prothoracic legs, which can temporarily disrupt normal
walking activity. Old age does not limit the animals' ability to climb
obstacles or right themselves. However, aged cockroaches show deficits in
inclined walking and escape behavior. Reduction in motor activity in aged
animals could be the result of deterioration in the musculature
(Anderson, 2003;
Rantanen, 2003
), sensory
neurons (Bergman and Ulfhake,
1998
; Ulfhake et al.,
2002
) and/or motor neurons
(Parkes et al., 1999
).
Deficits in each of these areas may have a significant or negligent effect
during different behaviors. Our goal in this paper was to examine behavioral
changes with age during many different types of locomotion in an effort to
ultimately address physiological and anatomical changes that may be occurring
in the nervous and musculoskeletal systems.
American and death-head cockroaches have similar adult lifespans and
show reduced activity levels with increasing age
Our results are similar to other studies that have recorded the lifespan of
adult cockroaches (Griffiths and Tauber,
1942; Pope, 1953
;
Willis and Lewis, 1957
;
Willis et al., 1957
). There
are a number of factors that could influence the lifespan of cockroaches
including temperature, diet, reproductive experience, and degree of isolation
(Griffiths and Tauber, 1942
;
Pope, 1953
;
Willis and Lewis, 1957
;
Willis et al., 1957
). However,
we have limited this variability by raising each of these cohorts under the
same environmental conditions (temperature and diet).
Our findings that cockroaches show a decrease in spontaneous motor activity
with age are consistent with studies in other insects
(Fernandez et al., 1999;
Le Bourg, 1987
;
Parkes et al., 1999
;
Tofilski, 2000
) and mammals
(Godde et al., 2002
;
Hilleras et al., 1999
;
Scimonelli et al., 1999
;
Siwak et al., 2002
). In light
of this study and others, the degree of spontaneous locomotion seems to
provide an accurate measure of age in a number of animals.
Aged animals walk normally but often develop `tarsus catch'
Although gait disruption was often present in aged animals, individuals
were able to recover the tripod gait within one leg cycle. In the stick insect
(Carausius morosus), it has been shown that coordinating influences
at the level of the CNS are sufficient to regain normal coordination in the
step directly following an interrupted step
(Cruse and Schwarze, 1988).
Therefore, degeneration at the level of the CNS could reduce the ability of
the animal to recover the tripod gait after the perturbation
(Pearson, 2000
). Furthermore,
changes in the placement of the legs during horizontal walking could be the
result of degeneration in the CNS of aged animals. Differences in leg
placement were subtle and do not appear to contribute to tarsus catch. These
changes might simply be the result of stiff and inflexible joints. In elderly
humans, alterations of gait often occur as a result of arthritis in the leg
joints (Elble et al., 1991
;
Mesure et al., 1999
). However,
further analysis is needed to determine whether changes in the CNS with aging
are contributing to these gait changes.
Cockroaches with `tarsus catch' also have hardening of the tarsal pads.
These animals often have difficulty grasping the walking surface. The tarsi of
insects provide friction with the substrate and are important in the
generation of propulsive forces (Betz,
2002; Dai et al.,
2002
; Frazier et al.,
1999
). In order to generate sufficient adhesion on inclines, the
tarsal pads exude adhesive materials and also deform their surface to expand
over the substrate (Jiao et al.,
2000
). The hardening of the tarsal pads seen in aged cockroaches
might be associated with decreases in the amount of adhesive that is released
and most certainly reduces the capacity of the pad to deform on the surface.
However, this deficit only causes temporary changes in gait and does not seem
to affect the animals' ability to walk on a horizontal surface. These findings
suggest that `tarsus catch' is most likely to be the result of degeneration at
the periphery.
Aged cockroaches are able to climb an obstacle, but postural changes
are sometimes absent
When young and old cockroaches climb over small obstacles (less than 6 mm),
the front legs reach the top of the block during normal swing movements of the
leg (Watson et al., 2002).
However, successful climbing of larger obstacles requires that the COM is
raised to the height of the obstacle
(Watson et al., 2002
). Aged
cockroaches are able to climb obstacles of up to 16.5 mm in height. The
majority of aged animals show rearing behavior when climbing blocks greater
than 6 mm. However, a small percentage of aged animals ran into the side of
the block (head butt) before surmounting it. Cockroaches assess an obstacle,
using visual or antennal inputs, in order to accurately change the body
posture and place the foot on top of the block
(Watson et al., 2002
).
Preliminary studies in our laboratory have suggested that the antennae play
the primary role in the detection of the obstacle during climbing. Age-related
structural changes in the insect brain, including loss of nerve cells,
alteration in cell structure and decreased metabolic rate, have been
documented (Kern, 1986
).
Therefore, it is possible that old animals that run into the obstacle have
degeneration in the antennae detection system.
Aged cockroaches are able to right, but animals with `tarsus catch'
take longer
Stiffness in the leg joints and reduced mobility could negatively affect
righting. However, degeneration in the higher CNS of aged animals would not
necessarily affect righting behavior. Decapitated cockroaches are readily able
to right themselves, and the mean duration of righting was only 12% greater
than in intact cockroaches (Camhi,
1977).
Leg movements during righting are often uncoordinated and unpredictable
(Camhi, 1977;
Full et al., 1995
). Although
we did not systematically look at leg movements in aged animals during
righting, previous studies (Sherman et
al., 1977
) have shown that cycle frequency of leg muscle activity
and inter-leg coordination are similar during walking and righting. However,
tactile and load feedback from the legs is altered from that of standing
(Camhi, 1977
;
Sherman et al., 1977
).
Differences in sensory feedback result in shorter burst duration of leg
muscles and decreased spike frequencies during righting
(Sherman et al., 1977
).
Studies by Camhi (1977
) showed
that animals in which all six legs were amputated at the mid-coxal level did
not show righting behavior. These findings illustrate that sensory information
from the periphery is important for normal righting behavior. Our behavioral
experiments showed that aged animals are able to right, although the duration
of righting is longer when compared with that of younger animals. Therefore,
either these aged animals do not have extensive sensory degeneration at the
periphery or some types of peripheral degeneration do not have significant
effects on the success of righting behavior.
Success in inclined walking is limited in aged individuals
On a horizontal surface, cockroaches generally walk with a tripod gait.
However, our studies have shown that aged and young cockroaches use a
metachronal gait to successfully climb an incline of 45°. By contrast,
aged animals that were unable to climb the incline often had uncoordinated
gaits. In a metachronal gait, more legs are on the ground at one time. This
would increase the frictional forces between the legs and the surface and help
to compensate for increases in load on the animals from the steeper slope
(Pelletier and Caissie, 2001).
Similar alterations of gait in sloped walking have been reported in potato
beetles (Leptionotarsa decemlineata;
Pelletier and Caissie, 2001
)
and cats (Felis domesticus;
Carlson-Kuhta et al., 1998
).
During inclined walking, gravitational forces that oppose uphill movement are
acting on the body. Therefore, alterations of posture and gait are often
required to move an animal forward
(Carlson-Kuhta et al., 1998
;
Pelletier and Caissie, 2001
).
In insects, force receptors on the legs would be important in the detection of
changing gravitational forces during inclined walking
(Duysens et al., 2000
;
Noah et al., 2001
).
In our analysis, the presence of `tarsus catch' was not a good predictor of
failure in inclined walking. However, leg slipping was extensive in trials in
which animals failed to climb the incline. Slipping could simply be due to
age-related degeneration of the tarsal pads. Several studies have shown that
pad deformation and tarsal secretion are of vital importance in attachment on
smooth surfaces (Betz, 2002;
Jiao et al., 2000
). Adhesive
properties of the tarsal pads are maximal when the contact area of the pad is
large (Jiao et al., 2000
).
Indeed, the expansion of the pad is critical for developing sufficient
adhesive force for climbing. Therefore, hardening of the tarsal pads may
severely limit the ability of aged animals to grasp the surface. `Tarsal
catch' may well be an extreme condition of tarsal degeneration that begins at
earlier ages. Thus, even those individuals that do not exhibit `tarsal catch'
may have more limited pathologies in tarsal pad structure that would lead to
slipping.
Degeneration of leg proprioceptors could also contribute to problems in
incline walking. Leg slipping could certainly be detected by proprioceptive
sensors on the leg, which could have a direct effect on the motor output and
the gait pattern (Ridgel et al.,
1999,
2001
).
Escape behavior is absent in aged cockroaches
Age-related changes in the escape behavior were evident in Periplaneta
americana. Aged animals did not escape to stimuli that normally evoked
coordinated responses, and there was a loss or reduction of spontaneous
locomotion. However, after decapitation, tactile stimulation of senescent
animals evoked responses that are typical of headless cockroaches of younger
ages (Schaefer and Ritzmann,
2001). Age-related changes in higher centers may disrupt normal
descending inputs, which are important in modulation, coordination and
adaptation of leg movements (Grillner et
al., 2000
; Kien and Altman,
1992
). These findings suggest that descending inputs contribute to
the observed age-related changes in escape behavior and that the thoracic
circuitry and leg musculature are still capable of functioning `normally' in
the absence of descending inputs.
Sources of age-related behavioral deficit
This study has documented behavioral deficits in a range of locomotory
behaviors that are readily performed by aged cockroaches. We have discussed
potential sources of these deficits, which include CNS, peripheral nervous
system, cuticle or skeletal problems. However, more research is required to
identify the exact pathologies that lead to each of these problems.
Each of the behavioral deficits that we observed in aged animals is complex and could result from deficits in more than one of the areas listed above. Horizontal walking trials suggest that the behavioral deficits seen in aged cockroaches are the result of peripheral damage to the tarsi and the tibio-tarsal joint. However, analysis of transitional behaviors provides evidence that deficits in the CNS, especially the higher centers, may be present in aged cockroaches and these could certainly contribute to the problems seen in horizontal walking, albeit in a more subtle way. Anatomical analysis of the nervous system in old insects would complement these behavioral findings and could further elucidate the causes of locomotor changes with aging. A reasonable strategy for future research in this area would include anatomical studies of the nervous system and peripheral structures along with continued behavioral observations of a range of behaviors that document deficits associated with various different conditions. Moreover, a thorough understanding of the behavioral deficits associated with aging and the related structural pathologies could serve as naturally occurring lesions that lead to a greater understanding of the intact system.
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Acknowledgments |
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References |
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