Differing mechanisms of cold-induced changes in capillary supply in m. tibialis anterior of rats and hamsters
1 Department of Physiology, Cumhuriyet University, 58140 Sivas, Turkey and
2 Angiogenesis Research Group, Department of Physiology, University of Birmingham Medical School, Birmingham B15 2TT, UK
*Author for correspondence (e-mail: s.egginton{at}bham.ac.uk)
Accepted 3 January 2002
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Summary |
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Key words: angiogenesis, capillary, histochemistry, enzyme activity, fibre size, rat, Rattus norvegicus, hamster, Mesocricetus auratus.
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Introduction |
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When tissue oxygenation becomes inadequate or oxygen demand rises sufficiently, an increase in vascularity may be required to promote oxygen delivery to cells. This will reduce the distance over which diffusion occurs, increase capillary surface area for exchange and accommodate a greater maximum local blood flow for delivery (Hudlická et al., 1992). In addition to its role in peripheral oxygen transport to the tissues, the capillary supply is also important for the removal of metabolic end-products such as CO2 and lactic acid, and for the maintenance of local thermal balance. The capillary supply may therefore play an important role in the strategies of both cold avoidance and cold-acclimation. Many approaches have been used to study the vascularisation of muscles in mammals, usually showing that individual muscle fibres are surrounded by a number of capillaries in proportion to their oxidative metabolic capacity (Romanul, 1965
).
Exposure to cold is thought to increase the capillary supply to skeletal muscle of rats (Heroux and St Pierre, 1957), guinea pigs (Sillau et al., 1980
) and mice (Wickler, 1981
), but these data are ambiguous because of the methodologies employed (Egginton, 1990a
, 1998
). In addition, isolating the relevant stimuli may be difficult, e.g. increased capillarisation at high altitude may reflect hypoxia, cold exposure or a combination of these stimuli. When animals were exposed to cold, an increase in capillarisation of skeletal muscles was observed (Banchero et al., 1985
). It was expected that cold plus hypoxia would be more effective in inducing capillarisation than cold alone because of the synergistic effect of increasing metabolic demand by cold and reducing oxygen supply by hypoxia. However, the response depended on muscle fibre composition since cold alone was most effective in increasing capillarisation in soleus muscle (slow oxidative muscle), while cold plus hypoxia was more effective in the gastrocnemius muscle (a mixed muscle) (Banchero, 1982
).
The basal metabolic rate of rats is lower than that of hamsters at a common environmental temperature (Himms-Hagen, 1986), so these non-hibernator and hibernator species may respond differently to cold exposure. Given a common stimulus, we reasoned that species with different over-wintering strategies would also show differing physiological responses to a period of gradual cooling to overcome the potential limitation in peripheral oxygen transport at low environmental temperatures, the process of cold-acclimation. Such comparisons are difficult to make on the basis of published data because the response evoked by cooling or cold exposure varies among species, and depends on the animals age and previous thermal history (duration and/or depth of cold exposure), leading to conflicting reports in the literature on the effects of cold exposure. An experimental protocol was therefore adopted that followed gradual alterations in both temperature and photoperiod in order to mimic seasonal fluctuations (summer/winter transition) rather than acute exposure to low temperatures, as used previously, and exposed non-hibernator and hibernator rodents to the same conditions. As far as we are aware, this is the first time such an approach has been adopted. Preliminary data have been presented in abstract form (Deveci and Egginton, 1996
, 1998
).
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Materials and methods |
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Cold-acclimation
To minimise any intra-specific allometric (scale-dependent) effects, we compared groups of animals with similar body mass. While this meant starting the 8-week acclimation period with juvenile rats, the changes observed would presumably be accentuated by acclimating older (hence larger and having a reduced intrinsic capacity for non-shivering thermogenesis) animals, and we therefore feel this compromise was justified.
The initial body mass of cold-exposed rats was 6070 g, and that of hamsters was 130140 g, with final body masses of approximately 260 and 170 g, respectively. Rearing conditions were an ambient temperature of 21±1°C and daily photoperiod of 12 h:12 h light:dark (L:D); control animals were maintained under these conditions. Following Home Office guidelines for experimentation, hamsters were housed in individual cages in an environmental chamber whereas rats were kept two or three in a cage. The initial air temperature on transfer was 20°C, and this was gradually reduced to 5°C, accompanied by a photoperiod reduced by 1.5 h each week from an initial setting of 8 h:16 h L:D to 1 h per day by week 4. After 4 weeks and up to 8 weeks, photoperiod was held at 1 h:23 h L:D, and a temperature of 5±1°C was maintained.
Histochemistry
At the end of the cold-acclimation period, the m. tibialis anterior (TA) was quickly removed from one side of animals under anaesthesia and weighed, and slices from the proximal one-third were frozen in isopentane cooled in liquid nitrogen for subsequent histological processing. Serial 10 µm cryostat sections were stained to demonstrate myofibrillar ATPase (for fibre typing), succinic dehydrogenase (for oxidative capacity) and alkaline phosphatase (for capillary endothelium) activities. We did not distinguish between Type IIb and Type IId/x/c fibres (see Fig. 1), whose oxidative capacity is between those of Types IIa and IIb (Schiaffino et al., 1990). Thus, the oxidative capacity of hamster TA may have been slightly underestimated by fibre typing relative to oxidative enzyme activity. Capillary supply was evaluated as the numerical capillary-to-fibre ratio (C:F) or capillary density (CD; mm2), and muscle composition was evaluated as relative fibre type density and mean cross-sectional area, using an unbiased sampling protocol (Egginton, 1990a
,b
).
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The distance over which oxygen is transported is one of the most important limiting factors in the supply of oxygen from a capillary to the tissues, accentuating the resistance to oxygen flux existing at the capillary/fibre interface (Egginton, 1990a). We estimated diffusion distance (D) according to the formula of Rakusan and Poupa (1963
) as: D=500/
CD, where CD is capillary density. We also used a formula recommended by Snyder (1987
) for hexagonal capillary arrays which estimates the maximum (ninety-fifth percentile) oxygen diffusion distance as Rmax=[(0.326+0.312)/C:F]
FCSA and the mean oxygen diffusion distance as Rave=[(0.140+0.231)/C:F]
FCSA. Clearly, such formulae are sensitive to both cold-induced angiogenesis and changes in fibre size since CD will vary reciprocally with FCSA.
Enzyme activity
Muscles contralateral to those obtained for histochemical analysis were quickly removed, frozen in liquid nitrogen and stored at 80°C until analysis. Samples were homogenised in a 30-fold excess (w/v) of ice-cold buffer (2 mmol l1 MgCl2, 1 mmol l1 EDTA, 50 mmol l1 imidazole, pH 7.4 at 20°C). Measurement of enzyme activity was performed at 25±0.1°C under optimal conditions of pH and substrate/cofactor concentrations, using substrate deletion as a control for background activity, monitored by following the oxidation of the pyridine nucleotide NADH at 340 nm (using a millimolar extinction coefficient, EmM, of 6.22) or the reduction of 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) at 412 nm (EmM=13.6). The results are expressed as micromoles of product formed per minute per gram wet mass. The anaerobic capacity of muscle was evaluated by assaying phosphofructokinase (PFK) and lactate dehydrogenase (LDH) activities, the aerobic capacity by the activity of citrate synthase (CS) as a marker for the citric acid cycle and 3-hydroxyacyl-CoA dehydrogenase (HAD), which is a marker of fatty acid catabolism (ß-oxidation).
Phosphofructokinase (EC 2.4.1.1)
PFK activity was determined in the presence of 1.5 mmol l1 NADH, 7 mmol l1 MgCl2, 2 mmol l1 AMP, 2 units ml1 -glycerophosphate dehydrogenase, 0.5 units ml1 aldolase and 75 mmol l1 triethanolamine-HCl buffer, pH 8.2 at 25°C. The reaction was initiated by addition of ATP and D-fructose 6-phosphate to the medium, and PFK activity was determined at 340 nm.
Lactate dehydrogenase (EC 1.1.1.27)
LDH was assayed as pyruvate reductase in a medium consisting of 1.5 mmol l1 NADH, 50 mmol l1 imidazole-HCl buffer, pH 7.4 at 25°C. The reaction was initiated by addition of 3 mmol l1 sodium pyruvate, and LDH activity was measured at 340 nm. The pyruvate concentration that gave the highest LDH activity was determined for each tissue.
Citrate synthase (EC 4.1.3.7)
The assay medium consisted of 2.5 mmol l1 DTNB, 5.0 mmol l1 oxaloacetic acid (OAA), 3.0 mmol l1 S-acetyl CoA, 50 mmol l1 triethanolamine-HCl buffer, pH 8.0 at 25°C. CS activity was determined at 412 nm after the addition of 0.3 mmol l1 acetyl coenzyme A (sodium salt), following subtraction of background deacylase activity from activity in the presence of OAA.
3-Hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)
HAD activity was determined in the presence of 1.5 mmol l1 NADH, 1 mmol l1 EDTA and 50 mmol l1 imidazole buffer, pH 7.4 at 25°C; the reaction was initiated with 2.0 mmol l1 acetoacetyl coenzyme A (sodium salt), and followed at 340 nm.
Statistical evaluation
All results are given as means ± S.E.M. Analysis of variance (ANOVA) was used to determine statistical significance (taken to be P<0.05), using Fishers PLSD test for multiple comparisons.
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Results |
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Fibre type composition
The compositions of muscles in euthermic rats and hamsters were distinct (Table 2), but the relative distributions of fibre types among regions within the TA were quite similar for both species (Fig. 3A,B). The TA was composed mainly of Type IIb (fast glycolytic, FG) fibres, with a smaller proportion of Type IIa (fast oxidative glycolytic, FOG) and a few Type I (slow oxidative, SO) fibres. The numerical density (NN) of fibre types showed modest alterations between euthermic and Cold-A groups in either species, although the modest inter-specific differences were reduced further on cold-acclimation, nor were there any significant differences in the areal density of fibre types (Table 2). The muscles showed no evidence of oedema or necrosis, with the interstitium occupying approximately 5 % of muscle cross section in all groups. Fibre cross-sectional area (FCSA) was the parameter most affected by cold-acclimation, showing a general hypertrophy in rat muscles and atrophy in hamster muscles compared with euthermic groups. Mean FCSA increased significantly in the Cold-A rat from 1980±112 to 2512±210 µm2 (P<0.05, see Fig. 5A), while it declined in Cold-A hamsters from 2346±88 to 2035±108 µm2 (P<0.05, see Fig. 5B). Differences in mean FCSA were due primarily to the response of fast muscle fibres (mainly Type IIb) in both species (Fig. 3A,B).
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The estimated intramuscular diffusion distances (D) were similar for euthermic and Cold-A rats: D=19.8±1.3 µm compared with 19.3±0.9 µm (not significant), Rmax=25.5±1.3 µm compared with 25.6±1.4 µm (not significant), Rave=14.3±0.9 µm compared with 13.9±0.8 µm (not significant) for euthermic compared with Cold-A, respectively. In contrast, values were significantly smaller in Cold-A hamster TA: D=16.8±0.5 µm compared with 14.7±0.4 µm (P<0.01), Rmax=22.9±0.6 µm compared with 21.1±0.6 µm (P<0.05) and Rave=12.0±0.3 µm compared with 11.1±0.3 µm (P<0.05). Regional differences were also evident, with diffusion distance lower in the core than the cortex in both species, reflecting both the smaller FCSA and the higher CD. While these differences among regions were more evident in the rat, cold-acclimation did not alter calculated diffusion distance. However, all measures were reduced in both regions of the TA in Cold-A hamsters (Fig. 6).
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Discussion |
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Enzyme activity
There are few metabolic data available that compare hibernator with non-hibernator animals under similar experimental conditions. During hibernation of ground squirrels, LDH activity increased and CS activity decreased in plantaris muscle (Steffen et al., 1991). In contrast, we looked at changes occurring during the process of cold-acclimation that precedes hibernation and found no significant alteration in activities of the oxidative marker enzymes CS and HAD in either species. Similarly, although higher mitochondria volume density and higher cristae surface density have been reported in Cold-A rat muscles (Buser et al., 1982
), the effective mitochondrial mass, as indicated by the content of mitochondrial protein and of cytochrome oxidase activity per gram of muscle, was not altered (Behrens and Himms-Hagen, 1977
). In addition, an increase in the maximal rate of muscle oxygen consumption upon cold-acclimation and exercise in guinea pigs revealed no increase in mitochondrial volume, suggesting no change in oxidative capacity (Hoppeler et al., 1995
).
To combat the cold, rats may stimulate anaerobic metabolism in skeletal muscle as a result of shivering thermogenesis. Blood lactate levels have been shown to increase in Cold-A guinea pigs (Turner et al., 1995), so an increase in LDH activity may allow increased gluconeogenesis. However, the somewhat lower activity of the flux-limiting enzyme PFK in Cold-A rats strongly suggests that shivering thermogenesis was not fuelled by glycolysis in the present study. Depression of PFK activity may be a result of the change in intracellular adenylate charge ([ATP]/[ADP] ratio) since the enzyme is inhibited by high levels of ATP and H+, which prevents excessive formation of lactate and a precipitous drop in blood pH. PFK inhibition also occurs by accumulation of citrate, an indication that biosynthetic precursors are abundant and additional glucose need not be degraded for this purpose (Newsholme and Leech, 1983
). The increased LDH activity in rat fast muscles may therefore result from hyperkinesia and greater fast glycolytic (Type IIb) fibre activity during cold-acclimation. In contrast, hamsters show little change in either LDH or PFK activities and are therefore unlikely to rely on shivering thermogenesis.
Enzyme ratio alterations typically paralleled changes in absolute enzyme activities in both species during cold-acclimation, suggesting a broad maintenance of metabolic pattern. The PKF/CS activity ratio, indicating relative glycolytic rather than oxidative capacity, suggests that the former was greater in rats than in hamsters and that this was not significantly affected by cold-acclimation. Interestingly, there was a clear reduction in fast skeletal muscle HAD/CS activity ratio during cold-acclimation of hamsters, indicating a reduced reliance on lipid metabolism during cold-acclimation. In contrast, significantly larger numbers of fat droplets were observed in both diaphragm and soleus muscle in rat, indicating a shift to lipids as a source of energy in oxidative muscles of Cold-A animals (Buser et al., 1982). The lack of change in HAD activity in our study of fast skeletal muscle would argue against sparing of fatty acids as a substrate for non-shivering heat production via UCP in more glycolytic muscle. This is consistent with elevated UCP2 mRNA levels in BAT of both cold-exposed and hibernating ground squirrels (Boyer et al., 1998
). Mobilisation of lipid depots to BAT, of which the hamster has a greater relative mass than the rat (D. Deveci and S. Egginton, unpublished data), rather than muscle would be a more efficient strategy for non-shivering thermogenesis in hamsters.
Fibre type composition
Histochemical staining of rodent skeletal muscle identifies large differences in the metabolic profiles of three major fibre types. Importantly, Type IIa fibres possess high activities of both glycolytic and oxidative enzymes and may be more oxidative than Type I fibres (Saltin and Gollnick, 1983). In the TA muscle, two different regions can be distinguished, a predominantly glycolytic cortex and a mainly oxidative core. In rat, 33 % of fibres are Type IIa in the TA core compared with 22 % in the slow oxidative soleus, while in hamster the values are 26 % and 30 % respectively.
Increased muscle activity can induce fibre type transformation from fast glycolytic to fast oxidative phenotypes (Andersen and Henriksson, 1977). An additional stimulus for muscle fibre transformation in a tissue-specific manner is hormone levels, for example with slow muscle being more sensitive than fast muscle to thyroid status (Izumo et al., 1986
). Thyroid hormone (T3) activity and basal metabolic rate were increased in cold-exposed rats (Storm et al., 1981
) and hamsters (Sigurdson and Himms-Hagen, 1988
). Why, then, despite observing behavioural changes in muscle activity (i.e. shivering thermogenesis or hypokinesia), was there little evidence of fibre transformation in the TA? It may be that the sensitivity of fibre transformation varies with hormonal status. For example, stimulation and hyperthyroidism had antagonistic effects on myosin expression in rat fast-twitch muscle (Kirschbaum et al., 1990
), while high resistance training decreased Type IIb myosin heavy chain (MHC) content in the medial gastrocnemius muscle, but again this transformation was blunted by T3 administration (Caiozzo et al., 1995
). Fibre composition expressed as relative number and area was qualitatively similar in the present study since cold-acclimation did not affect the size difference among fibre types. Moreover, cold-induced hypertrophy and atrophy did not alter these indices in the TA cortex of rat and hamster, respectively, suggesting an adaptive change in muscle mass that is tightly regulated to preserve the original muscle composition or relative fibre size.
Fibre size
In spite of little or no fibre transformation, the predominantly glycolytic TA of conspicuously active rats showed a significant hypertrophy in response to lowered temperature and light levels. This may be the result of a combination of factors including cold-induced hyperthyroidism, increased metabolic and sympathetic activity, shivering and/or an age-dependent response. Muscle hypertrophy reflects an increase in protein content resulting from both greater synthesis and reduced degradation rates, while the inverse is true of atrophied muscle (Goldberg et al., 1975). However, in the present study, rat fast muscle showed hypertrophy under conditions where the animals were allowed to undertake a physiological adaptation to gradually reduced temperature, rather than the reported pathological atrophy in response to a sudden exposure to low temperatures (typically 56°C in other studies) and exposure to an appropriate photoperiod (usually a constant 12 h:12 h L:D). This hypertrophy was primarily due to an increased FCSA of Type II fibres in the cortex and core regions and may be stimulated by the anabolic effect of cold-induced hyperthyroidism, although thyroid hormone has catabolic effects at higher doses (Tata et al., 1963
), and preventing transformation from fast to slow fibre types. The lack of Type I fibre response suggests either that slow fibre malleability in response to cold-induced stimuli was less than that of fast fibres or that induction of hypertrophy depended on the original fibre size.
Whole muscle and/or muscle fibre atrophy has been reported in most studies of cold exposure in the rat (Heroux and St Pierre, 1957; Snyder et al., 1992
; Suzuki et al., 1997
), in guinea pig (Sillau et al., 1980
; Aquin et al., 1980
) and in pigeon (Mathieu-Costello et al., 1998
). However, these studies used larger rats than we did (body mass 200300 g, compared with our initial mass of 60 g), so there may be a compounding allometric response. Cold exposure in early life may be less disruptive of growth because the inherent potential for non-shivering thermogenesis provides some defence against low environmental temperature. In contrast, adult animals will adopt a resistance to the adverse effects of cold exposure rather than adaptations that permit continuance of normal anabolism, leading to retardation of growth. It may be that the progressive cold exposure in our experiment produced more continuous shivering than the acute cold exposure used previously, leading to hypertrophy of the TA in cold-acclimated rats. This may parallel the condition induced by short trains of tetanic contractions when the response of fibre metabolism was dissociated from that of capillary supply (Egginton and Hudlická, 2000
). The muscle regions with larger fibres were the most superficial, so they were also likely to be subjected to a larger thermal gradient and to be stimulated most by shivering activity. Our experimental procedure increased the time the nocturnal rats were awake, but stimulated the onset of hibernation in the hamster (previous observations have indicated that this occurs between 10 and 12 weeks with our protocol). The corresponding increase in activity in the rat could therefore also have contributed to muscle hypertrophy.
In contrast, the mean FCSA of hamster TA was significantly lower after cold-acclimation, mainly due to atrophy of Type II fibres. Hamsters were observed to have a reduced level of motor activity and thus showed modest atrophy, in agreement with other studies on hibernator animals (Wickler et al., 1991; Steffen et al., 1991
). It is well known that hypokinesia, as seen during the hibernation period, causes muscular atrophy (Fitts et al., 1986
). Disuse atrophy during cold-acclimation was evident, particularly during the last 4 weeks at low temperatures and short photoperiod, when the animals spent most of the time sleeping in their nest. Short photoperiod and cold together are more effective as a stimulus for hibernation than either alone (Hoffman, 1968
). Shivering is an asynchronous stimulus, resulting in isometric exercise of both flexor and extensor muscles, and may have contributed to the hypertrophy of rat TA. The hamster, in contrast, has a very large non-shivering thermogenic capacity and the gradually reduced photoperiod and temperature may not cause shivering but prepare the animal for hibernation. One reason for hibernation is to avoid adverse environmental conditions, such as scarcity of food, and hence hypometabolism usually occurs in parallel with lower feeding rates (Hoffman, 1968
). Thus, atrophy of hamster muscle may additionally be due to inadequate nutrition during the preparation for hibernation. Decreasing FCSA has been demonstrated in other hibernators, such as the ground squirrel (Musacchia et al., 1989
), and this atrophy was accompanied by decreased protein synthesis during hibernation. However, in the present study, animals were presumably able to improve their oxygen-carrying capacity (e.g. haematocrit) (Deveci et al., 2001a
) and substrate extraction (capillary supply) before atrophy became established, whereas in other studies acute cold exposure would have allowed inadequate time for compensatory changes to be made. The response to a sudden increase in metabolic activity, due to either physical activity (rat) or metabolic thermogenesis (hamster), therefore involved reducing oxygen diffusion distances (i.e. increasing CD or reducing FCSA).
Capillary supply
In the thermoneutral zone (ambient temperature Ta=2729°C), rat and hamster basal metabolic rates are similar, although in response to progressively lowering environmental temperature the metabolic rate of hamsters increased much more than that of rats at the same temperature (Himms-Hagen, 1986). Mortola (1991
) reported that the resting rate of oxygen consumption of adult hamsters was 44 % higher than that of rats, presumably supporting non-shivering thermogenesis. Our data also support the hypothesis that oxygen demand is matched with oxygen supply: hamsters had a higher blood oxygen-carrying capacity than rats (Deveci et al., 2001a
), but also a higher oxidative enzyme activity and an overall higher capillarity in the TA. Although aerobic marker enzymes and fibre type composition changed little during cold-acclimation, Cold-A animals may better exploit this capacity. Under physiological conditions, increased muscle capillarity may be induced by increased blood flow via its effects on shear stress and wall tension (Hudlická et al., 1992
), although a metabolic stimulus for angiogenesis is compelling (Adair et al., 1990
).
In a survey of previous studies on capillary growth (angiogenesis), Hudlická et al. (1992) concluded that, in addition to the widely reported influence of cytokines and growth factors, capillary growth may also be elicited by mechanical factors associated with increases in fibre girth. Fibre cross-sectional area (FCSA) data are useful for distinguishing between an apparent increase in capillarisation (CD), as a result of fibre atrophy, and true angiogenesis. Regional analysis showed that there was a good correlation between the site of angiogenesis and FCSA within the TA muscle of rats. In the cortex region, where mean FCSA was largest (>2250 µm2), the relative change in C:F was approximately double that of the core region (FCSA 1600 µm2).
Heroux and St Pierre (1957) first reported apparent capillary growth in rats following cold exposure, although their results were ambiguous because of the method used to identify capillaries and the interpretation of results on the basis of CD (Egginton, 1998
). Similar conclusions followed more detailed studies subsequently performed on guinea pigs by Sillau et al. (1980
) and Banchero et al. (1985
). Likewise, angiogenesis has not been observed in the rat (Snyder et al., 1992
; Suzuki et al., 1997
) or pigeon (Mathieu-Costello et al., 1998
) upon cold-acclimation to a Ta of 5°C. These researchers all reported an increase in CD, but this was due entirely to muscle fibre atrophy since the C:F did not change. In addition to the possible allometric component, these authors exposed the rats to a sudden drop in temperature, whereas we gradually lowered temperature and reduced the duration of the light phase of the photoperiod, simulating natural conditions (summer/winter transition). In both cases, a cold-induced increase in basal metabolic rate will increase muscle oxygen demand. In the present study, this was offset by increasing oxygen supply after stimulating angiogenesis, whereas in the other cited examples rats were subjected to a cold shock rather than a natural adaptation and increased oxygen demand was offset by reducing oxygen diffusion distance as a result of muscle fibre atrophy.
In general, the TA showed a different response both within muscle (cortex and core) and between species. In Cold-A rats, the TA cortex and core C:F increased significantly by approximately 40 % in the former and by half that in the latter, but CD showed no significant change. In contrast, Cold-A hamster CD increased significantly in both cortex and core (by approximately 33 %), but the C:F did not change. In hamster, the increased CD was due to a significant decrease in FCSA rather than to capillary growth, whereas the increased C:F in rat was due to the new capillary growth which paralleled an increase in FCSA. It is reasonable to assume that these new capillaries compensate at least partially for the increase in diffusion distance that occurs as the capillaries are pushed apart by fibre hypertrophy. The lack of angiogenic stimuli in hamster may result from two important adaptive responses, the absence of significant shivering in hamster muscles and the reduction in FCSA/increased CD which reduced oxygen diffusion distances sufficiently to prevent an adequate metabolic error signal developing.
The greatest change in capillarisation was seen in the TA cortex region of Cold-A rat, induced by cold-induced shivering, which also showed the greatest degree of fibre hypertrophy. The data from the TA cortex support our hypothesis that cold-induced shivering may be considered as a parallel to chronic low-intensity exercise. Muscle stimulation using a regime that activated glycolytic, but not oxidative, fibres (very low voltage and short trains of tetanic contractions) caused capillary growth in the glycolytic cortex of the TA muscle but not in the oxidative core, without any increase in the activity of cytochrome oxidase (Egginton and Hudlická, 2000). Moreover, the first appearance of an increased C:F has been shown to occur in the vicinity of glycolytic fibres of stimulated muscles (Hudlická et al., 1982
). Collectively, these data suggest that larger fibres are more responsive to stimulation. It has been assumed that glycolytic fibres are subjected to hypoxia during activity and that this stimulates angiogenesis. Although direct measurements of oxygen tension in the glycolytic region of rabbit TA using oxygen electrodes did not demonstrate any changes that could explain capillary growth as a result of increased muscle activity (Hudlická and Schroeder, 1978
; Kanabus et al., 1980
), more recently we looked at regional differences within muscles and observed that chronic systemic hypoxia induced angiogenesis where muscle fibre size was largest (Deveci et al., 2001b
). The angiogenic potential therefore scaled directly with oxygen diffusion and, hence, should be low in the TA core where the higher CD produces shorter diffusion distances and the fibres are unlikely to become hypoxic.
In hamster TA cortex and core regions, there were no significant changes in C:F, but CD responded to cold to the same extent (by approximately 33 %) as fibre atrophy. Clearly, an alternative angiogenic signal to activity or FCSA is operative, presumably hormonal, in origin. For example, hyperthyroidism may stimulate new capillary growth, possibly via a mitochondrial pathway since a high-affinity mitochondrial receptor for T3 has been shown to regulate the synthesis and activity of enzymes involved in oxidative phosphorylation (Roodyn et al., 1965; Sterling et al., 1978
). It is a striking example of an integrated physiological adaptive response that elevating T3 levels, leading to higher basal metabolic rate and some of the secondary morphological changes that appear in the cold such as improved capillary supply, may aid substrate delivery to, and removal of metabolic end-products from, active muscle.
Concluding remarks
Small rodents are limited in their ability to increase insulation when faced with low environmental temperatures and thus rely on changes in their basal metabolic rate. This may ultimately take the form of either a hypermetabolic (rat) or hypometabolic (hamster) strategy to survive prolonged exposure to cold. The species-specific responses reported in the present study may be explained as follows. Shivering thermogenesis as part of a cold-avoidance strategy would tend to increase capillarisation in fast muscles of rats. In contrast, the ability of hamsters to tolerate a lower core temperature by increased BAT activity under the same conditions would not be expected to increase capillarisation in skeletal muscle. However, altered photoperiod and gradual cold-acclimation may also invoke hormonal changes that would counter the progressive lowering of activity and also increase capillarisation in rat, but in hamster the increased capillary density was due to cold-induced atrophy of muscle fibre cross-sectional area. In addition, within distinct regions of the TA, there was a correlation between angiogenesis and fibre size in the rat, in which oxygen diffusion increased, but not in the hamster, in which there was a reduced oxygen diffusion distance.
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Acknowledgments |
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References |
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