Calcification in the planula and polyp of the hydroid Hydractinia symbiolongicarpus (Cnidaria, Hydrozoa)
Department of Biology, The University of North Carolina at Charlotte, Charlotte, NC 28223, USA
*Author for correspondence (e-mail: mbthomas{at}email.uncc.edu)
Accepted May 14, 2001
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Summary |
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Key words: aragonite, biomineralization, calcium, calcium carbonate, coral, larva, spicule.
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Introduction |
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In another subclass of Anthozoa, the ahermatypic, non-reef building gorgorian corals, calcium carbonate is in the form of spicules that create an internal skeleton. These spicules are formed within vacuoles of sclerocytes located in the mesoglea. Subsequently, the sclerocytes migrate to, and deposit the spicules into, the supporting axis (Simkiss and Wilbur, 1989).
Although calcification has been studied for several decades, early studies were limited to the physiology and ecology of calcification, and to structural studies for taxonomic purposes. More recently, several studies have attempted to answer questions concerning the underlying cellular mechanisms of calcification. The kinetics of calcium uptake and the incorporation of calcium into calcium carbonate skeletons have been described for Leptogorgia virgulata (Lucas and Knapp, 1997), Stylophora pistillata (Tambutte et al., 1996; Allemand et al., 1998), Corallium rubrum (Allemand and Tambutte, 1996), Tubastrea faulkneri and Galaxea fascicularis (Marshall, 1996). These studies suggest a transcellular pathway for calcium to reach cells involved in calcification (Tambutte et al., 1996), involving voltage-gated, L-type calcium channels (Tambutte et al., 1996), and Ca2+-ATPases in the plasma membrane (Marshall, 1996; Kingsley and Watabe, 1984).
In addition, Lucas and Knapp (Lucas and Knapp, 1997) studied the incorporation of bicarbonate, the other ion required for calcium carbonate formation, into coral skeletons of L. virgulata. They proposed a model for the mechanism by which calcium carbonate crystals are formed. According to their model, bicarbonate ions may be made available for the production of calcium carbonate in three ways. The first is by diffusion of dissolved CO2 from the extracellular fluid into the cell. Once inside the cell, H2O and CO2 combine in the presence of carbonic anhydrase to form carbonic acid (H2CO3), which spontaneously loses a hydrogen ion to form the bicarbonate ion (HCO3-). The second method is through metabolically generated CO2. Carbon dioxide generated from oxidation of metabolites in the citric acid cycle is converted by carbonic anhydrase into carbonic acid, from which a hydrogen ion dissociates to form a bicarbonate ion. The final method is simply the transport of dissolved bicarbonate from the extracellular fluid into the cell by bicarbonate transporters in the cell membrane.
In addition to biomineralization, calcium plays a major role in second messenger systems, where it controls exocytosis, cortical reactions in eggs and muscle contraction (Alberts et al., 1994). In the marine hydroid Hydractinia symbiolongicarpus, calcium is required for larval motility (Ball, 1996), induction of metamorphosis (Thomas et al., 1997) and secretion of adhesive material during metamorphosis (Ball, 1996).
The hydrozoan H. symbiolongicarpus forms polymorphic colonies that occur on the shells of pagurid hermit crabs, usually Pagurus longicarpus. The life cycle of organisms in the genus Hydractinia has been described (reviewed by Hyman, 1940). Within 24h of fertilization, the embryo develops into a free-swimming larva, the planula, capable of undergoing metamorphosis. Following receipt of a metamorphic stimulus, typically a bacterial product in nature or other agents, including cesium, administered experimentally (reviewed by Thomas and Edwards, 1997), the planula shortens, attaches to a substratum, and undergoes biochemical and morphological changes to become a primary polyp. Apical tentacles grow out around a newly formed mouth and stolons grow out along the substratum. As these stolons extend new polyps form at intervals and eventually differentiate into specialized polyps that are responsible for feeding, reproduction and defense. Colony growth continues until the substratum, typically the surface of the shell of a hermit crab, is covered.
We observed that a crystalline mat occurred beneath the stolons of colonies growing on microscope slides. The existence of this crystalline material raised the possibility that this hydroid might share with corals the ability to produce calcium carbonate. Lucas and Knapp (Lucas and Knapp, 1997) showed that calcium carbonate spicules in vacuoles of sclerocytes of gorgonian corals were visible by polarized light microscopy. When colonies of H. symbiolongicarpus were examined by polarized light and differential interference contrast (DIC) microscopy, which employs polarized light, crystalline structures of varying sizes were observed moving, probably by Brownian motion, within the vacuoles of the gastrodermal cells. Crystals were also observed in gastrodermal cells of planulae. We hypothesized that these crystals were also a calcium salt, possibly calcium carbonate.
The purpose of this study was threefold: (1) to examine to occurrence of the crystalline structures throughout the life cycle of H. symbiolongicarpus; (2) to determine the composition of the crystalline mat beneath the colony and the crystals within the vacuoles of gastrodermal cells; and (3) to explore the cellular mechanisms that underlie calcification in this species through use of various inhibitors and modulators.
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Materials and methods |
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Analysis of composition of crystalline mat by infrared spectrometry
To determine the composition of the crystalline mat below the stolons of H. symbiolongicarpus, polyps were transplanted to glass microscope slides (Buss et al., 1984) and allowed to grow for 20 months. After 20 months, the tissues of the transplanted colonies were removed by immersing the colonies in 5.25% sodium hypochlorite (Clorox bleach; Kingsley and Watabe, 1984; Lucas and Knapp, 1997; Marshall, 1996) for 24h. Afterwards, loose particles of the crystalline mat were vacuum-filtered from the bleach solution onto 22µm biological filters and rinsed 5 times with glass-distilled water. The crystals were oven-dried at 125°C for 48h.
The crystals were analyzed on a BioRAD FT-IR Spectrometer 175C using the KBr press method. A sample of commercially available CaCO3 (Sigma) was analyzed as a control. Samples were mixed with KBr and a pure KBr press was used as a reference. The spectra obtained were analyzed using BioRad FTS software and compared to spectra previously obtained by others (Adler and Kerr, 1962; Kikuchi and Tamiya, 1984).
Analysis of composition of vacuolar crystals and mechanisms of formation
To elucidate the composition and examine the cellular mechanisms involved in the formation of the crystals located in the vacuoles of the gastrodermal cells of H. symbiolongicarpus, we manipulated the ions in the environment and used various inhibitors to determine the effect of altering the amount of available calcium and carbonate on the formation of crystals. These quantitative experiments were performed on planulae, rather than polyps, because of the enhanced ability to control the levels of available calcium and carbonate and the relative ease in maintaining the specimens in the treatments.
For all experiments testing the effects of different treatments on the number of crystals per planula, embryos were reared in 35x10mm plastic Petri dishes. It was necessary to rear planulae in ASW alone for the first 6h, since planulae 06h old exhibit high sensitivity to treatment solutions, particularly those manipulating calcium levels, which often results in mortality or extreme deformities. To maintain a consistent experimental protocol, planulae were transferred to all treatment solutions 68h after fertilization and were then reared in treatment solutions until 4850h post-fertilization. Treatment solutions were changed daily, except for nifedipine, which was changed every 8h owing to its instability in aqueous solution. Planulae can creep along a substratum by both ciliary and muscular motility or attach themselves to the substratum with mucus secreted from the anterior end, and remain motionless; left undisturbed, they tend to assume the latter behavior. Thus, to reduce behavioral variation, both experimental and control planulae were kept undisturbed in the dark.
Quantification of crystals
At 48h, a squeeze preparation of 36 planulae from each treatment group were observed with a Zeiss Axioskop using polarized light optics and video-recorded. Only planulae of normal size and exhibiting normal development were video-recorded. Still photos were captured (in JPEG file format) using ZipshotTM and Arc PhotoImpressionTM software on a Hewlett Packard Pavilion PC (8370) or Hewlett Packard Pavilion Notebook (N3250). Since no significant difference was found in the size distribution of crystals between treatments, the number of crystals in each planula was counted to quantify calcification. Three replicates of each treatment were performed.
Data from each of the three replicates were analyzed for significance using two-way ANOVA without correlation. In all cases, no significant difference (P=0.312) was found among the replicates of the treatments. However, a significant difference (P<0.001) was found between the treatments. To determine which treatments were significantly different from the appropriate controls, several pairwise multiple comparison procedures were used, including Tukeys Test, the StudentNewmanKeuls Method, and the Bonferroni t-test. In addition, Students t-test was also used to demonstrate significance. A P-value of less than 0.05 was considered to be significant. All statistical analyses were performed on SigmaStat 2.03 software. Results are presented in the text as mean ± S.D. of the pooled group; in graphs, values are means ± S.E.M. Significance is shown as the results of the Tukeys test.
Chemicals used
Acetazolamide, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), diltiazem, nifedipine, ryanodine and verapamil were dissolved in undiluted dimethylsulfoxide (DMSO) to produce 10mmoll-1 stock solutions. The stock solutions were diluted in ASW for use. The final concentration of DMSO never exceeded 1%. Caffeine readily dissolves in ASW and a 10mmoll-1 stock solution was prepared that was then diluted in ASW for use. Procaine and Ruthenium Red require vigorous agitation to dissolve in ASW. All chemicals were purchased from Sigma except for ryanodine, which was purchased from Calbiochem.
Sea water with elevated calcium (50mmoll-1 Ca2+) was prepared by mixing 52ml of 0.34moll-1 CaCl2, 17ml of 0.53moll-1 KCl, 62ml of 0.37moll-1 MgCl2, 28ml of 0.90moll-1 MgSO4, 3.98ml of 0.54moll-1 NaHCO3, and then adding dry NaCl and water to bring the final volume to 1000ml with a salinity of 35 p.p.t. (modified from Woods Hole Formulae and Methods, 5th edition, 1964, Marine Biological Laboratory, Woods Hole, MA, USA). Ca2+-free sea water was prepared by omitting CaCl2 from the previous formula.
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Results |
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The effects of an inhibitor of Ca2+-ATPases was explored by rearing larvae in 0.1mmoll-1 Ruthenium Red (Fig.6). Ruthenium Red significantly reduced the number of crystals produced in larvae to 295±93, or 60±19% of the ASW control (P<0.001; N=12).
To determine the effects of modulators of ryanodine-sensitive intracellular calcium stores on crystal production, planulae were treated with 0.1mmoll-1 caffeine, 0.001mmoll-1 ryanodine, or 0.001mmoll-1 procaine (Fig.6). Caffeine has been shown to induce the release of calcium from the sarcoplasmic reticulum through ryanodine receptors (RyR; Dutro et al., 1993; Iizuka et al., 1998; Shimoda et al., 2000). In this study, caffeine reduced the mean number of crystals produced in larvae to 202±103, or 41±38% of the ASW control (P<0.001; N=15). Ryanodine and procaine, both inhibitors of the release of calcium from intracellular stores through RyR receptors in vertebrate cells (Dutro et al., 1993; Iizuka et al., 1998; Shimoda et al., 2000), also significantly reduced crystal production in the planulae of H. symbiolongicarpus. The mean number of crystals formed in planulae reared in 0.001mmoll-1 ryanodine was 243±51, or 49±10% of the ASW control (P<0.001; N=11) and 46±10% of DMSO control (P<0.001; N=11). The mean number of crystals formed in planulae reared in 0.001mmoll-1 procaine was 331±49, or 67±10% of the ASW control (P<0.01; N=12).
Requirement for carbonate in crystal formation
Manipulation of bicarbonate in seawater is difficult because of the effects of atmospheric CO2. To test whether or not the crystals contained bicarbonate, an attempt was made to reduce the intracellular concentrations of bicarbonate by treatment of larvae with acetazolamide, a carbonic anhydrase inhibitor (Maren, 1977), and by treatment with DIDS, an inhibitor of bicarbonate transport (Madshus, 1988). The results of these studies are included in Fig.6 and Table1.
Embryos were treated with 0.01mmoll-1 acetazolamide in 0.1% DMSO or 0.001mmoll-1 DIDS in 0.01% DMSO and reared to 48h. Groups of embryos reared in 1% DMSO and in ASW served as controls. The number of crystals formed in 0.01mmoll-1 acetazolamide was significantly reduced to 273±133, or 55±27% of the ASW control (P<0.001; N=13) and 51±25% of the DMSO control (P<0.001; N=13). The number of crystals formed in planulae exposed to 0.001mmoll-1 DIDS was also significantly reduced to 234±90, or 48±18% of the ASW control (P<0.001; N=14) and 44±17% of the DMSO control (P<0.001; N=14).
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Discussion |
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The crystalline mat contains calcium carbonate
Analysis of the crystalline mat using IR spectroscopy identified the crystals as calcium carbonate. Calcium carbonate can occur in several different crystalline forms, the two most common being calcite and aragonite, which can be distinguished by their IR spectra (Adler and Kerr, 1962; Kikuchi and Tamiya, 1984). Calcite and aragonite have very similar crystal structures; the calcium ions of both are located in almost the same lattice positions in layers, alternating with layers of carbonate ions. The major difference between the two is in the arrangement of the carbonate ions (Falini et al., 1996). Although calcite is the more stable form of calcium carbonate at normal temperatures and pressures (Falini et al., 1996), aragonite is more popular in biological structures. Aragonite is the prevalent form in coral reefs and in the shells of mollusks (Kikuchi and Tamiya, 1984). By comparing the IR spectra obtained in this study with those obtained in previous studies (Adler and Kerr, 1962; Kikuchi and Tamiya, 1984), we determined that the crystals in H. symbiolongicarpus are calcium carbonate in the form of aragonite.
Vacuolar crystals of gastrodermal cells contain calcium carbonate
Our data suggest that the free-floating vacuolar crystals contain calcium. Alteration of calcium levels in surrounding sea water and manipulation of calcium levels by the use of inhibitors of calcium transport yielded the results expected if the crystals are made of calcium, that is they either significantly increased or reduced crystal production.
Methods to demonstrate the presence of carbonate are not as readily available. There are few, if any, cytochemical methods for staining bicarbonate or carbonate, and it is difficult to reliably alter carbonate levels in surrounding sea water, owing to the exchange of CO2 between sea water and the atmosphere. In this study, therefore, we attempted to alter bicarbonate levels available for calcium carbonate production by using acetazolamide, an inhibitor of carbonic anhydrase activity (Maren, 1977), and DIDS, an inhibitor of anion transporters (Madshus, 1988). Both of these inhibitors significantly reduced crystal production in planulae of H. symbiolongicarpus, indicating that bicarbonate is a component of the crystals.
Cellular mechanisms involved in calcification
Inhibitors of L-type calcium channels and Ca2+-ATPases
Voltage dependent L-type calcium channels have been isolated and cloned from scleractian coral, where they have been shown to be associated with the calicoblastic epithelium, the site involved in calcium carbonate precipitation (Zoccola et al., 1999). The ability of inhibitors of L-type calcium channels to inhibit crystal formation in H. symbiolongicarpus suggests that calcium channels are involved in calcification in this hydroid as well. Nifedipine, verapamil and diltiazem were also shown to inhibit calcium incorporation into the skeleton of S. pistillata (Tambutte et al., 1996). Verapamil also has been shown to inhibit calcium incorporation into the skeletons of C. rubrum (Allemand and Grillo, 1992) and G. fascicularis, but not T. faulkneri (Marshall, 1996). Diltiazem was the most effective in reducing crystal production in H. symbiolongicarpus and also exhibited the highest degree of inhibition of calcium incorporation into the skeleton of S. pistillata (Tambutte et al., 1996). Inhibitors of N- and T-type calcium channels were shown to be ineffective inhibitors of calcification in S. pistillata (Tambutte et al., 1996).
In addition to calcium channels, several studies demonstrate the role of Ca2+-ATPases in calcification in corals as well as in H. symbiolongicarpus. Ruthenium Red inhibited calcium incorporation in G. fascicularis and T. faulkneri (Marshall, 1996), and vanadate, another Ca2+-ATPase inhibitor, was shown to inhibit calcification in L. virgulata (Kingsley and Watabe, 1984).
Modulators of ryanodine-sensitive calcium stores: caffeine, ryanodine, procaine
Two types of intracellular calcium stores have been identified; those that are inositol trisphosphate (InsP3) sensitive and those that are ryanodine sensitive (Iizuka et al., 1998; Shimoda et al., 2000). Caffeine, ryanodine and procaine, all modulators of ryanodine-sensitive calcium stores, were effective inhibitors of crystal formation in H. symbiolongicarpus. Caffeine, which significantly reduced crystal production, induces the release of calcium from ryanodine-sensitive intracellular stores (Iizuka et al., 1998; Shimoda et al., 2000). The reduction of crystal production by treatment with caffeine was possibly due to depletion of the calcium from the calcifying vacuole.
The inhibitory effect of ryanodine and procaine on crystal formation in H. symbiolongicarpus cannot be as easily explained. In vertebrate cells, ryanodine and procaine are both inhibitors of the release of calcium from ryanodine-sensitive stores (Dutro et al., 1993; Iizuka et al., 1998; Shimoda et al., 2000). It was predicted, therefore, that treatment of planulae with ryanodine or procaine would result in near normal or even increased crystal production, rather than the decrease that was observed. However, there is evidence that, in invertebrates, ryanodine has the opposite effect on ryanodine-sensitive calcium stores, so that instead of inhibiting the release of calcium, ryanodine induces the release of calcium from ryanodine-sensitive stores. Lea (Lea, 1996), who performed his studies on the shore crab Carcinus maenas and the lobster Homarus vulgaris, did not exclude the possibility of a separate ryanodine-releasable calcium store or a second isoform of the ryanodine receptor with different properties from the classic ryanodine receptor. Additionally, ryanodine has been demonstrated to release calcium from intracellular stores in sea urchin eggs (Buck et al., 1994). Unfortunately, there are no other studies of the effects of caffeine, ryanodine, or procaine on calcification in other Cnidaria for the purpose of comparison.
Thapsigargin, which induces the release of calcium from InsP3-sensitive stores, was not an effective inhibitor of calcification in S. pistillata (Tambutte et al., 1996), T. faulkneri and G. fascicularis (Marshall, 1996).
Inhibitor of carbonic anhydrase: acetazolamide
Carbonic anhydrase has been shown to play a central role in calcification in corals (Kingsley and Watabe, 1987; Lucas and Knapp, 1996). It can aid in calcification by catalyzing the hydration of CO2 resulting in the production of HCO3-. It can also catalyze the conversion of HCO3- to CO2, the reverse of the above reaction, thus reducing the acidity of the environment and making it more conducive for calcification (Kingsley and Watabe, 1987). Cytochemical studies have demonstrated high levels of carbonic anhydrase activity on the membrane of spicule-forming vacuoles and in electron-dense bodies that are hypothesized to contain materials used in spicule formation in L. virgulata (Kinsley and Watabe, 1987).
Acetazolamide (Diamox), a specific inhibitor of carbonic anhydrase (Maren, 1977) effectively reduced crystal production in H. symbiolongicarpus, suggesting that carbonic anhydrase plays a role in calcification. Acetazolamide reduced the incorporation of calcium and carbonate into the skeletons of C. rubrum (Allemand and Grillo, 1992), L. virgulata (Lucas and Knapp, 1997) and S. pistillata (Tambutte et al., 1996).
Inhibitor of anion transporters: DIDS
The anion transporter inhibitor, DIDS (Madshus, 1988), was an effective inhibitor of crystal formation in H. symbiolongicarpus. There are several proposed methods for the action of DIDS, including the inhibition of electrogenic Cl- flux coupled with Ca2+ flux (Yasumasu et al., 1985) or the inhibition of Na+/HCO3- symporters (reviewed by Madshus, 1988). The latter method has been implicated in the inhibition of calcification by DIDS in C. rubrum (Allemand and Grillo, 1992), L. virgulata (Lucas and Knapp, 1997), and S. pistillata (Tambutte et al., 1996).
Calcification in the planulae and polyps
Rather than evolving solely as a supporting structure, it has been hypothesized that the calcium carbonate skeleton of corals developed as a calcium sink for the removal of waste calcium that was subsequently adapted for structural support (Lowenstam, 1981; Constanz, 1986). We propose that in H. symbiolongicarpus both functions are exhibited, but in different stages of the life cycle.
The planula utilizes calcium sequestration as a physiological sink. The data obtained from the present study, together with data obtained from previous cytochemical studies (A. M. Dandar, C. L. Rogers and M. B. Thomas, manuscript submitted for publication), suggest the following hypothetical model for calcification in the planulae of H. symbiolongicarpus. Septate junctions, which are tight junctions found primarily in invertebrates (reviewed by Thomas and Edwards, 1991), occur between ectodermal cells of H. symbiolongicarpus (C. L. Rogers and M. B. Thomas, personal observation), suggesting that calcium does not enter the planula by a paracellular route. It is more likely that calcium passively diffuses into the ectoderm cells by L-type calcium channels for use in motility, secretion of mucus or metamorphosis. Subsequently, in order to maintain a low intracellular concentration of calcium, calcium is pumped, by Ca2+-ATPases, out of ectodermal cells into the intercellular space. Since, according to our model, gastrodermal cells take up excess calcium, the intercellular space would have a lower concentration of calcium than the surrounding sea water. It would therefore be more energy-efficient for the planula to pump calcium into the intercellular space than into calcium-rich sea water. To maintain a lower level of calcium in the intercellular spaces, calcium is then sequestered in an insoluble form in the gastrodermal cells. The cytochemical studies by Dandar et al. (A. M. Dandar, C. L. Rogers and M. B. Thomas, manuscript submitted for publication) suggest that calcium first enters and accumulates in the cytosol and nucleus of the gastrodermal cells. At a later stage, calcium that accumulates in the cytosol and nucleus is translocated into the vacuole. Bicarbonate moves by facilitated diffusion into the vacuoles from either the seawater or from bicarbonate produced intracellularly, depending on the activity of carbonic anhydrase. This process involves DIDS-sensitive anion transporters. In the vacuoles, calcium combines with bicarbonate to form calcium carbonate. This model presents a mechanism for sequestering calcium in an insoluble form, an energy-efficient means of ridding the organism of excess calcium.
After the planula metamorphoses into a polyp, the cellular mechanism for sequestering calcium is retained and adapted to form a crystalline mat. Crystals occur in the endoderm of the stolons. Whether they are produced in the stolons or reach them by migration of the epithelium, resulting from mitosis of cells of the body column, as has been shown for other hydroids (Davis, 1973; reviewed by Thomas and Edwards, 1991), is currently under investigation. These crystal-containing cells in the stolons appear to contribute to the formation of the crystalline mat underlying the colony and the mechanism of formation seems to involve death of stolons. We observed dead, crystallized remains of stolons running throughout the crystalline mat, suggesting that death of the crystal-containing cells leads to the formation of a crystalline conglomerate. This proposal is supported by the observation that when an entire colony dies, it leaves behind a crystalline skeleton of polyps and stolons. The exact cause of the death of the stolons in a living colony is unknown. It is probably due to blockage of the gastrovascular tubes, but we cannot rule out the possibility of a more controlled process such as apoptosis.
In addition to calcification in the dead gastrovascular tubes, inorganic precipitation and growth of crystals occurs as described in the stony coral, where seed crystals, produced in specialized cells, are deposited into the skeleton mass and act as a nucleus for the deposition of additional calcium carbonate (Constanz, 1986).
The function of the crystalline mat underlying the colony is unclear. The mat may simply be the final product of excretion of excess calcium. Preliminary evidence, however, indicates that colonies that are unable to produce the crystalline mat over long periods of time lose their ability to adhere firmly to the substratum. An alternative or additional function may be to increase surface area for colony growth, which is limited for an organism whose substratum is the surface of a shell occupied by a hermit crab. The shell itself, of course, cannot grow. If, however, the hydroids release calcium carbonate onto the surface of the shell, they can increase the surface area available to them. Our calculations for a shell typical of those colonized by H. symbiolongicarpus (9x13mm) indicate that if a colony created a crystalline mat 0.5mm thick, the surface area would increase from 544 to 610mm2. Since the average density of the polyps is 1.48polypsmm-2, the modified substratum would accommodate 45 more polyps, which could contribute positively to the reproductive success of the colony.
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Acknowledgments |
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