Effect of photoperiod on body mass, food intake and body composition in the field vole, Microtus agrestis
1 Aberdeen Centre for Energy Regulation and Obesity (ACERO), School of
Biological Sciences, Zoology Building, University of Aberdeen, Aberdeen AB24
2TZ, UK
2 BioSS Office, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB,
UK
3 ACERO, Division of Energy Balance and Obesity, Rowett Research
Institute, Bucksburn, Aberdeen AB21 9SB, UK
* Author for correspondence (e-mail: e.krol{at}abdn.ac.uk)
Accepted 6 December 2004
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Summary |
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Key words: photoperiod, energy balance, digestive efficiency, organ morphology, de novo lipogenesis, fatty acid composition, Siberian hamster, Syrian hamster, collared lemming, field vole, Microtus agrestis
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Introduction |
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The responses to changing photoperiod can be readily induced in the
laboratory by simulating the natural progression of day lengths or by acutely
transferring animals between summer (long) and winter (short) day lengths.
This makes hamsters, voles and lemmings attractive study species for
investigations in which biological rhythms may provide valuable insights into
mechanisms underlying regulation of body mass
(Mercer and Speakman, 2001;
Bartness et al., 2002
), eating
disorders (Morgan et al.,
2003b
) or infertility
(Bavister, 2002
).
Apart from reproductive activity, annual cycles of body mass and adiposity
are the most profound features of seasonality in mammals. Although these
robust rhythms occur in a variety of mammalian species, they have been studied
most extensively in the Siberian or Djungarian hamster (Phodopus
sungorus), Syrian or golden hamster (Mesocricetus auratus) and
collared lemming (Dicrostonyx groenlandicus). In the Siberian hamster
both sexes defend a maximal body mass (associated also with a significant
adipose tissue store) in summer and reduce their body masses in winter
(Steinlechner and Heldmaier,
1982; Bartness and Goldman,
1988
). The transfer of adult male hamsters (housed at room
temperature) from long day photoperiod (LD, 16 h:8 h L:D) to short day
photoperiod (SD, 8 h:16 h L:D) results in a gradual weight loss accompanied by
reduced food intake. After 1218 weeks, hamsters lose approximately
3040% of their initial body mass, with more than half of this being
attributed to mobilization of fat reserves
(Ebling, 1994
;
Klingenspor et al., 2000
;
Mercer et al., 2000
,
2001
). Decreases in body fat
reserves are a result of a reduction in adipocyte size, and affect
intraperitoneal white adipose tissue more than subcutaneous fat depots
(Bartness, 1996
). It has been
suggested that the seasonal changes in body mass and adiposity in Siberian
hamsters are mediated by defence of a sliding set-point (e.g.
Bartness et al., 1989
;
Mercer and Speakman, 2001
;
Morgan et al., 2003a
). Similar
seasonal changes (i.e. SD-induced decreases in body mass and adiposity) have
also been demonstrated in the European hamster (Cricetus cricetus;
Canguilhem et al., 1988
),
montane vole (Microtus montanus;
Petterborg, 1978
), meadow vole
(Microtus pennsylvanicus; Dark
and Zucker, 1984
), tundra vole (Microtus oeconomus;
Wang and Wang, 1996
) and bank
vole (Clethrionomys glareolus;
Peacock et al., 2004
).
By contrast, Syrian hamsters and collared lemmings have seasonal cycles in
body mass with maxima in the winter. In Syrian hamsters, the body weight gain
induced by exposure to SD reflects an increase in lipid stores and is more
pronounced in females than males (Bartness
and Wade, 1985). In response to SD photoperiod (10 h:14 h L:D),
adult female hamsters housed at room temperature increase body mass by
approximately 5060% over an 8 week period, with no concomitant increase
in food intake (Campbell and Tabor,
1983
). In collared lemmings, males maintained at 18°C and
weaned into SD (8 h:16 h L:D) grow to an adult body mass of 75 g, which
represents an 88% increase above the control level (40 g) achieved by males
weaned into LD (22 h:2 h L:D)(Reynolds and
Lavigne, 1989
; Hunter and
Nagy, 2002
). The corresponding values for females are 60 g in SD
and 35 g in LD. The increase in mass associated with exposure to SD at weaning
is the result of an increase in the absolute quantity of dry fat mass, dry
lean mass and body water mass (Nagy and
Negus, 1993
). After achieving adult mass, smaller LD male lemmings
exposed to SD conditions are able to increase body mass by a further 65% (from
40 g to 66 g) over a 11 week period, also with no increase in food intake
(Nagy, 1993
). In contrast to
immature lemmings, the body mass gain of adult animals results predominantly
from an increase in mass of lean tissue and body water
(Nagy et al., 1994
).
Here we present data on the field vole (Microtus agrestis) a novel model to investigate the regulation of body mass and energy homeostasis at both organism and molecular levels. We examined the effect of increased day length on body mass, food intake, apparent digestive efficiency and body composition in cold-acclimated male voles by transferring them from short to long day photoperiod. We also estimated the proportion of fatty acids synthesized de novo in adipose tissue of SD and LD voles to evaluate the contribution of dietary fat to the accumulation of adipose tissue triggered by the change in photoperiod. If the changes in de novo lipogenesis induced by photoperiod are substantial, they may affect the fatty acid composition of adipose tissue. Thus, another aim of this study was to compare fatty acid profile of fat depots in SD and LD voles.
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Materials and methods |
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The voles used in the experiment (83 males) were second and third generation born in captivity between 10 February and 20 April 2001. They were weaned at 3 weeks of age and kept in groups of 35 animals per cage. When the voles were 610 weeks old, they were transferred to a short day photoperiod (SD, 8 h:16 h L:D, lights on 08:40 h) at an ambient temperature of 8°C (range 7 to 9°C). We chose 8°C, since a preliminary study demonstrated that the response of voles to photoperiodic manipulation was more pronounced at this temperature than at 21°C. The voles were allowed to acclimate to these conditions for a total of 14 weeks (10 weeks of group housing and 4 weeks of individual housing). Bedding was provided for the first 2 weeks of the acclimation period and then removed.
Following the acclimation period, all animals were kept in SD for a further 24 days to obtain baseline measurements of body mass and food intake, after which 45 voles were exposed to a long day photoperiod (LD, 16 h:8 h L:D, lights on 04:40 h) at 8°C (range 7 to 9°C), whereas 38 control voles remained in original SD conditions. Both groups were matched for body mass and food intake, and monitored for up to 70 days following the exposure. To exclude the effect of particular cold room on body mass and food intake, we swapped the LD and SD voles between rooms on three occasions (days 7, 28 and 49 of exposure to LD) and adjusted photoperiod in the rooms accordingly.
Body mass was recorded twice per week, and food intake was measured over 3 days in each week. Some of the food intake data were also used to assess apparent digestive efficiency (N=23 voles). Following exposure to LD, we randomly selected 46 LD and 24 SD voles each week and dissected them for organ morphology. The same dissection protocol was applied to nine SD voles (three animals per week) that were sacrificed prior to exposure to LD. Fat depots of six LD and three SD voles were also used to determine fatty acid composition of adipose tissue. Consequently, each of the 83 voles used in the experiment had 228 measurements of body mass and 114 measurements of food intake (depending on when the animal was dissected), 01 measurements of apparent digestive efficiency and 01 determinations of fatty acid composition of adipose tissue. All voles were dissected to evaluate organ morphology. We investigated the effect of photoperiod on the proportion of fatty acids synthesized de novo in adipose tissue on a separate group of male voles (N=39) maintained under similar conditions.
Body mass, food intake and apparent digestive efficiency
Measurements of body mass (±0.01 g, Sartorius top-pan balance;
Epsom, Surrey, UK) were conducted between days 24 and 70 (where day 0
was the day of exposure to LD). Food intake was measured ±0.01 g,
Sartorius top-pan balance) between days 14 and 70. At the same time
each week, voles were weighed, placed in cages with fresh sawdust and provided
with a weighed portion of dry food. Three days later the animals were
re-weighed and any uneaten food was collected. Sorting through the sawdust of
23 cages (used in the digestive efficiency measurements) revealed that
spillage of food from the hoppers was negligible (0.8±0.4% of the food
removed from the hoppers over 3 days). The collected food was subsequently
dried and weighed to calculate dry food intake. Food was dried in a convection
oven at 60°C for 10 days, before it was given to the animals and after it
was collected from the cages to correct for changes in food water content
related to different levels of temperature and relative humidity in the food
storage (21°C, 97%) and animal rooms (8°C, 42%). During 4 day
intervals between the 3 day food intake measurements, all voles were fed with
non-dried food.
Apparent digestive efficiency was measured in voles exposed to LD for
1121 days (N=8) and 5363 days (N=8).
Simultaneous measurements of digestibility were also conducted on SD controls
(N=7). We collected the faeces produced by these animals during the 3
day feeding measurements and dried them at 60°C to a constant mass. The
gross energy content of food and faeces were measured by bomb calorimetry
(Gallenkamp Autobomb Adiabatic Bomb Calorimeter; Loughborough, Leicestershire,
UK). The apparent digestive efficiency was calculated as the percentage of
gross energy intake that was digested. We used the food intake and
digestibility data to calculate metabolizable energy intake, assuming that
urinary energy loss was 3% of the digestible energy intake
(Drod
, 1975
).
Body composition and organ morphology
Body composition (dry lean mass, dry fat mass, body fat and water content)
was derived from organ morphology data. Voles were weighed and killed by
CO2 overdose in the middle of the light phase. Following cardiac
puncture to collect blood, voles were immediately dissected. We removed the
brain, interscapular brown adipose tissue, white adipose tissue (anterior
subcutaneous, posterior subcutaneous, epididymal, perirenal, retroperitoneal
and mesenteric fat depots), thyroid, heart, lungs, gut, liver, pancreas,
spleen, kidney and gonads (testes and seminal vesicles). To ensure enough
tissue for further analyses, anterior and posterior subcutaneous fat depots
were pooled and are henceforth called subcutaneous fat. The gut was cut at the
pyloric and cardiac sphincters, the ileocaecal junction and the anus. The
excised stomach, caecum, and small and large intestines, were cut open
longitudinally to remove any residual gut contents and mucous. The remaining
body parts were divided into the pelage and the carcass including skeletal
muscle and bone. All organs, apart from the brains, which were used for other
analyses, were weighed (±0.0001 g, Ohaus Analytical Plus; Beaumont
Leys, Leicester, UK) to determine wet mass, then dried in a convection oven at
60°C for 14 days and re-weighed to determine dry mass. We took sub-samples
of subcutaneous fat depots (to determine fatty acid composition of adipose
tissue) from nine voles, immediately after recording the wet mass of the
organs, and weighed the remaining tissue. For these voles, dry mass of
subcutaneous fat depots was estimated from the wet mass of the tissue (before
the sub-samples were taken) and water content of the tissue that remained
after sub-sampling.
De novo lipogenesis
We estimated de novo lipogenesis from the incorporation of
deuterium into fatty acids of subcutaneous and epididymal fat depots in voles
over days 1024 (N=14) and 5266 (N=13) of the
exposure to LD. Simultaneous measurements were also conducted on SD voles
(N=12). Animals were injected intraperitoneally with approximately
0.2 g of deuterated water (2H2O, 9.4 atom%) and then
maintained on drinking water enriched with deuterium (0.06 atom%) for 14 days.
This protocol was designed to maintain stable 2H enrichment in the
body water at approximately 0.05% throughout the study. Blood samples were
taken from the voles twice (on days 7 and 14 following the injection) to
determine 2H enrichment in the body water. Blood samples
(5070 µl) were flame sealed into pre-calibrated Vitrex pipettes
(Modulohm A/S, Herlev, Denmark) immediately after collection and stored at
4°C until analysis was performed. After taking the second blood sample
(day 14), voles were sacrificed and subcutaneous and epididymal fat depots
removed. We also blood sampled and sacrificed nine unlabelled voles (three for
each photoperiod group) to determine background levels of 2H in the
body water and adipose tissue.
All fat samples were weighed (±0.0001 g, Ohaus Analytical Plus),
then dried in a convection oven at 60°C for 14 days (to evaporate inter-
and intra-cellular body water containing enriched 2H) and
re-weighed to determine the mass of water lost. We replaced the evaporated
body water (by soaking the dry tissue at room temperature for 48 h) in an
excess of distilled water (15 times the mass of water lost). This protocol was
developed in our laboratory to allow deuterium bound to labile hydrogen sites
in the tissue (hydroxyl and amino groups) to re-exchange with distilled water.
Consequently, the only 2H left in the tissue was that irreversibly
incorporated into lipids (covalent CH bond) during de novo
synthesis (Culebras and Moore,
1977; Haggarty et al.,
1991
). After the process of isotope equilibration, the fat samples
were dried at 60°C for a further 14 days and stored at this temperature
(to minimise the contamination by atmospheric hydrogen) until total lipid and
fatty acid extractions were performed.
Total lipids were extracted using a modification of the method of Bligh and
Dyer (1959). Dry samples of
fat tissue (100-200 mg) were macerated and dissolved in 24 ml
chloroform/methanol (2:1). After mixing the dissolved fat samples with water
(6 ml), the aqueous and chloroform/methanol phases were separated by
centrifugation (1500 g, 10 min). The aqueous layer was then
discarded and the lipid layer was filtered through a Whatman 1PS filter paper.
The solvents were evaporated at 40°C under vacuum. The resulting total
lipids (50 mg) were saponified with 1 ml 0.5 mol l-1 KOH in 95%
ethanol for 90 min at 100°C. After adding diethyl ether (9 ml) and water
(3 ml), the aqueous phase (containing saponifiable material) and ether phase
(containing non-saponifiable material) were separated by centrifugation (1500
g, 5 min). The aqueous layer was then acidified with 5 mol
l-1 H2SO4 and free fatty acids extracted with
9 ml hexane/diethyl ether (19:1). The samples were stored at 60°C until
further analysis was performed.
Blood and fatty acid samples were used for isotope-ratio mass spectrometric
analysis of 2H. Full details of the analysis of the water distilled
from blood samples are given in Speakman and Król
(2005). Each lipid sample was
sub-sampled 57 times. These sub-samples (1.01.3 mg) were put in
pressed silver capsules (4.0 x 3.2 mm, Elemental Microanalysis Limited,
Okehampton, UK) and immediately sealed. The capsules were then placed on the
carousel of a EuroEA40/2-IRMS solid autosampler (EuroVector, Milano, Italy),
attached to a standard EA 3000 elemental analyser (EuroVector, Milano, Italy).
The samples were flushed with helium and pyrolytically decomposed in a
high-temperature pyrolysis system (1300°C), in the presence of reactive
carbon (Gehre and Strauch,
2003
). The resulting hydrogen gas was carried in the helium stream
through a GC column to an open split sampling capillary and into the source of
an isotope ratio mass spectrometer (IRMS). We measured the
2H:1H ratios with a single-inlet IRMS (IsoPrime,
Micromass UK Ltd) containing an electrostatic energy filter that separates the
helium tail from the hydrogen peak.
We used isotopically characterized hydrogen gas (CP grade gases BOC Ltd) in
the reference channel of the IRMS. The reference gas was characterized every
three months relative to SMOW and SLAP
(Craig, 1961) supplied by the
IAEA. Each batch of blood and lipid samples was run adjacent to triplicates of
three laboratory standards to correct for day-to-day differences in mass
spectrometer performance. All isotope enrichments were measured in
per
mil relative to the working standards and converted to p.p.m., using
established ratios for these reference materials. The measures of isotope
enrichment in the samples were based on analysis of 5 (blood) or 57
(lipids) sub-samples; all subsequent calculations were performed on the mean
values.
The proportion of fatty acids synthesized de novo in adipose
tissue (%) was calculated as (EFA
bgEFA) / ((EBW
bgEBW) x 0.53), where EFA and
EBW are 2H enrichments (p.p.m.) of fatty acids
and body water in the labelled vole, and bgEFA and
bgEBW are background 2H enrichments (p.p.m.) of
fatty acids and body water in unlabelled animals. The constant 0.53 is the
factor accounting for the partial incorporation (18 H) of hydrogen atoms of
average fatty acids (34 H) from body water
(Jungas, 1968;
Guo et al., 2000
;
Haggarty et al., 2000
). Our
estimate of de novo lipogenesis includes all fatty acids synthesized
from carbohydrates that were accumulated in adipose tissue over 14 days.
Fatty acid composition
We determined fatty acid composition of subcutaneous fat depots in SD voles
(N=3) and LD voles exposed to long photoperiod for 17 (N=3)
and 59 days (N=3). Within 15 min after sacrificing the animals, the
tissue samples were frozen in liquid nitrogen and capped under a stream of
N2 to prevent oxidation of double bonds. The tissues were then
stored at 20°C until lipid extraction was performed. Total lipids
were extracted from adipose tissue as described above for the measurements of
2H incorporation. Fatty acid methyl esters (FAMEs) were prepared by
reacting total lipids (1020 mg) with dry methanol (0.5 ml) containing 2
mol l-1 HCL for 2 h at 100°C, and then dissolving the lipids in
1 ml hexane/diethyl ether (19:1). After mixing the dissolved FAMEs with water
(0.5 ml), the aqueous and hexane/diethyl ether phases were separated by
centrifugation (1500 g, 10 min) and the lipid layer dried by
passing through anhydrous Na2SO4. The solvents were
evaporated at 40°C under a stream of N2 and the residue FAMEs
were taken up in hexane containing 0.02% butylated hydroxytoluene (BHT).
FAMEs were analysed by a HP5890 gas chromatograph (Hewlett Packard, Sunnydale, CA, USA) using a DB-23 column, 30 m x 0.25 mm, with a 0.25 µm film thickness (J & W Scientific, Folsum, CA, USA). Samples (1 µl) were injected into a split injection system (1:16) and carried through the GC column in a helium stream. The GC temperature program was 160°C for 1 min, increasing by 10°C min-1 to 180°C (held for 3 min) and then increasing by 2°C min-1 to 220°C (held for 15 min). A total of 37 fatty acids were identified on the basis of retention times of their methyl esters as compared to fatty acid methyl ester standards (SupelcoTM 37 Component FAME Mix, Sigma-Aldrich, St Louis, MO, USA). The peaks were analysed using a HP3396A integrator (Hewlett Packard, Sunnydale, CA, USA).
Each fatty acid was expressed as a percent of all 37 fatty acids identified in the sample. Fatty acids below 1% of the total were excluded from further analysis. These included C6:0, C8:0, C10:0, C11:0, C12:0, C13:0, C14:0, C14:1, C15:0, C15:1, C17:0, C17:1, C18:4, C19:0, C20:0, C20:1, C20:2, C21:0, C20:3, C20:4, C20:5, C22:0, C22:1, C22:2, C22:3, C22:4, C23:0, C24:0, C22:5, C22:6 and C24:1.
Statistics
Data are reported as mean ± S.D. (N = number of
animals unless stated otherwise). The significance of temporal changes in body
mass and food intake in LD and SD voles was assessed by one-way repeated
measures analysis of variance (ANOVA). The Tukey post hoc test was
used when differentiation between days was required. The effect of photoperiod
on body mass and food intake was evaluated by two-way repeated measures ANOVA,
with group (LD versus SD) and day of exposure as factors. We used
one-way ANOVA followed by Tukey pairwise comparisons to test for differences
in gross energy content of faeces, apparent digestive efficiency, body
composition, organ morphology, 2H enrichment of body water,
2H enrichment of adipose tissue, de novo lipogenesis and
fatty acid composition between the groups. Differences between the groups in
food intake, metabolizable energy intake and dry masses of fat and gonads were
also tested by analysis of covariance (ANCOVA), with body mass as a covariate.
The group means were adjusted to a common body mass using the least mean
squares method. For measurements conducted on the same animals (2H
enrichment of body water on days 7 and 14, 2H enrichment of
subcutaneous and epididymal fat depots, de novo lipogenesis in
subcutaneous and epididymal fat depots) we used paired t-tests.
Relationships between these data were also described using Pearson
product-moment correlation coefficients. All data were tested for normality
and homogeneity of variance and were transformed and reanalysed if these
assumptions were not met. Arcsine-square-root transformations were performed
prior to analysis for percentage data (apparent digestive efficiency, body
water and fat contents, de novo lipogenesis and fatty acid
composition). All statistical analyses were conducted using Minitab for
Windows (version 13.31, Minitab Inc.)
(Ryan et al., 1985), apart
from two-way repeated measures ANOVAs (SigmaStat for Windows, version 3.00,
SPSS Inc.). Statistical significance was determined at P<0.05,
unless stated otherwise. All tests were two-tailed. Exact test values have
been omitted in the text for simplification and clarity of the presented
results.
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Results |
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Body mass of the 12 voles exposed to LD increased significantly throughout the experiment (P<0.001, Fig. 1A). Prior to LD exposure (days 24 to 0), vole body mass remained stable and averaged 25.6±3.3 g. Following LD exposure, body mass increased from a mean of 26.2±3.7 g on day 0 to 36.3±5.2 g on day 28 (all comparisons between days 11 to 28 and days 24 to 0, P<0.05). Over the next 4 weeks, body mass remained stable and averaged 37.7±5.4 g. Hence, the body mass trajectory of the voles eventually exposed to LD could be characterised by a stable pre-exposure phase (days 24 to 0), followed by an increase phase (days 1 to 28) and a subsequent plateau phase (days 29 to 56). The analysis of body mass changes repeated on five of the 12 LD voles that were monitored to day 70 confirmed the existence of these three distinct phases and indicated no changes in body mass between days 56 and 70. Therefore, the final plateau phase can be extended to day 70. In the 11 SD voles, body mass averaged 24.9±4.8 g at the beginning of the experiment (day 24) and 30.6±5.6 g on day 56 (Fig. 1A). The increase in body mass of SD voles was gradual but significant (P<0.001).
|
Since both LD and SD voles increased their body mass over time, we evaluated the effect of photoperiod on body mass by comparing group x day interactions (reflecting group differences in the patterns of body mass change) and main group effects (reflecting body mass differences averaged over the analysis interval) prior to LD exposure (days 24 to 0), during the increase phase (days 1 to 28) and during the final plateau phase (days 29 to 56). As expected, LD and SD voles did not differ in their pattern of day-to-day body mass changes or in their mean body mass prior to exposure to LD (interaction group x day, P=0.97; group effect, P=0.88; day effect, P<0.001), since at this stage all voles were in the same conditions. During the first 4 weeks of LD exposure (days 128), the LD voles exhibited substantially greater weight increases than SD voles (interaction group x day, P<0.001; group effect, P=0.09; day effect, P<0.001). Between days 29 and 56 of LD exposure, the pattern of body mass changes of LD voles was no longer significantly different from that of SD voles and the significant body mass differences between LD and SD animals stabilized at 7.5±0.3 g (interaction group x day, P=0.68; group effect, P=0.004; day effect, P<0.001).
Food intake
Food intake of the 12 LD voles monitored between days 24 and 56
increased significantly throughout the experiment (P<0.001,
Fig. 1B). Prior to LD exposure
(days 14 to 0), food intake remained constant and averaged
6.1±0.8 g day-1. Following LD exposure, voles increased
their daily food intake from a mean of 6.3±0.9 g on day 0 to
7.8±1.2 g on day 28 (all comparisons between days 14 to 28 and days
14 to 0, P<0.05). Over the next 4 weeks, food intake
stabilized at a level of 8.1±1.2 g day-1. The trajectory of
food intake for voles exposed to LD closely resembled the changes in body mass
(Fig. 1A,B). For the 11 SD
voles, daily food intake averaged 6.2±0.9 g on day 14 and
7.2±1.1 g on day 56 (Fig.
1B). This increase in food intake was gradual but significant
(P<0.001), and also mirrored the pattern of changes in body
mass.
We compared food intake of LD and SD voles using the same method as for body mass. Prior to exposure to LD, the two groups of voles did not differ in day-to-day changes in food intake or mean food intake (interaction group x day, P=0.54; group effect, P=0.61; day effect, P=0.009). During the first 4 weeks of exposure to LD (days 1 to 28), the increases in food intake observed in LD animals were significantly greater than in SD voles (interaction group x day, P<0.001; group effect, P=0.33; day effect, P<0.001). Between days 29 and 56 of LD exposure, the temporal changes in food intake did not differ between the two groups and difference in mean food intake stabilized at 1.1±0.1 g day-1 (interaction group x day, P=0.87; group effect, P=0.016; day effect, P<0.001).
To investigate the effect of photoperiod on food intake in relation to changes in body mass, we compared food intake of the 12 LD voles during the pre-exposure, increase and plateau phases, including body mass as a covariate in the analysis. After adjusting for differences in body mass, the effect of LD on food intake was not significant (interaction body mass x photoperiod, P=0.94; body mass effect, P<0.001; photoperiod effect, P=0.95) (Fig. 2A). Consequently, the rate at which voles ingested food during the pre-exposure, increase and plateau phases (adjusted to a common body mass of 32.7 g) averaged 7.2, 7.2 and 7.3 g day-1, respectively. This suggests that LD voles responded to increases in body mass by increasing their food intake, i.e. the increase in food intake following exposure to LD was entirely attributable to the changes in body mass.
|
Apparent digestive efficiency and metabolizable energy intake
The mean gross energy content of food was 18.16±0.12 kJ
g-1 dry mass (N=3 replicates). The gross energy content of
faeces did not differ between SD voles and LD voles during the increase or
plateau phases (P=0.47) and averaged 17.11±0.41 kJ
g-1 dry mass (N=23). The apparent digestive efficiency
averaged 74.2±5.5% (N=7) in SD voles, 81.4±5.5%
(N=8) in voles during the increase phase and 80.3±4.1%
(N=8) during the plateau phase. These means were significantly
different (P=0.031), with the digestive efficiency of LD voles during
the increase and plateau phases being higher than in SD animals
(P<0.05).
We calculated metabolizable energy intake (MEI) for the 12 LD voles monitored between days 24 and 56 during the pre-exposure (days 24 to 0), increase (days 1 to 28) and final plateau (days 29 to 56) phases using their food intake data (Fig. 2A) and the mean digestive efficiencies of 74.2, 81.4 and 80.3%, respectively. After adjusting for differences in body mass, the effect of LD on MEI was significant (interaction body mass x photoperiod, P=0.81; body mass effect, P<0.001; photoperiod effect, P=0.006), with MEI during the increase and plateau phases being significantly higher than during the pre-exposure phase (P<0.05) (Fig. 2B). The rate at which voles assimilated energy during the pre-exposure, increase and plateau phases (adjusted to a common body mass of 32.7 g) averaged 95.3, 103.7 and 102.9 kJ day-1, respectively.
Body composition and organ morphology
Wet mass, dry mass and water content of organs of all voles sacrificed
during the experiment are presented in supplementary material. Comparisons of
body mass, body composition and organ morphology in voles exposed to SD, LD
for 128 days (the increase phase) and LD for 2970 days (the
plateau phase) demonstrated that animals sacrificed during the final plateau
phase were significantly heavier than SD controls
(Table 1). This increase in
body mass was associated with significant increases in absolute amounts of all
body components, including dry fat mass, dry lean mass, and body water mass
(Fig. 3). On average, voles
exposed to LD for 2970 days had a significantly higher body fat content
(12.9%) than SD individuals (7.1%). This was associated with a significant
decrease in body water content, from 59.4% in SD voles to 53.3% in LD voles.
At the organ level, voles sacrificed during the plateau phase had absolute
increases in dry masses of brown adipose tissue, subcutaneous, epididymal,
perirenal and retroperitoneal fat depots, thyroid, lungs, caecum, liver,
kidney, gonads (testes and seminal vesicles), pelage and carcass. By contrast,
the differences in dry masses of mesenteric fat, heart, gut (stomach and small
and large intestines), pancreas and spleen were not significant between the LD
and SD voles.
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|
Since organ size frequently correlates with body mass (e.g.
Selman et al., 2001;
Król et al., 2003
), we
repeated the analyses of body composition and organ morphology using body mass
as a covariate. As expected, most of the body components (apart from spleen,
pancreas, large intestine and caecum) were positively related to body mass
(P<0.001). After adjusting for body mass, the differences in dry
fat mass between LD and SD voles were not significant (interaction body mass
x photoperiod, P=0.29; body mass effect, P<0.001;
photoperiod effect, P=0.14) (Fig.
4). The effect of photoperiod on body composition and organ
morphology was significant only for gonads (interaction body mass x
photoperiod, P=0.65; body mass effect, P<0.001;
photoperiod effect, P<0.001), with voles exposed to LD for
2970 days having bigger gonads than SD controls or voles exposed to LD
for 128 days (P<0.05)
(Fig. 5). The dry mass of
gonads adjusted to a mean body mass of all voles (30.7 g) averaged 0.172 g in
SD animals, 0.207 g in LD voles sacrificed during the increase phase and 0.330
g in LD voles at the plateau phase.
|
|
De novo lipogenesis
Background levels of deuterium in body water and fatty acids extracted from
subcutaneous and epididymal fat depots did not differ between SD voles and LD
voles during the increase or plateau phases
(Table 2). After pooling the
data for all unlabelled animals (N=9), the 2H content of
body water, subcutaneous and epididymal fat depots averaged 144.6±0.4,
124.6±0.9 and 124.6±0.8 p.p.m., respectively. The difference
between the two adipose tissues was not significant (P=0.98).
However, the background level of 2H in fat depots was 1821
p.p.m. below that of the body water (P<0.001). This relative
2H depletion in lipid has been reported previously (e.g.
Haggarty et al., 2000) and is
the result of isotope discrimination against heavy hydrogen during reductive
biosynthesis of fatty acids (Jungas,
1968
).
|
Deuterium enrichment of body water measured after 7 days of dosing with the isotope did not differ between SD voles and LD voles during the increase or plateau phases (Table 2). Similarly, the differences between the three groups in body water 2H enrichment measured at the end of the labelling period (day 14) were not significant either. The deuterium content of body water across all labelled voles (N=39) averaged 479.4±21.7 p.p.m. on day 7 and 482.2±20.1 p.p.m. on day 14, with no significant changes between the two days (P=0.30). Thus, the stable plateau enrichment of 2H was maintained for at least the last seven days of the experiment. The data from both days were highly correlated (r=0.69, P<0.001).
Deuterium incorporation into fatty acids of subcutaneous fat depots measured at the end of the labelling period (day 14) did not differ between SD voles and LD voles during the increase or plateau phases (Table 2). The effect of photoperiod on 2H enrichment of epididymal fat depots was also not significant. When data were pooled for all labelled voles (N=39), the 2H enrichment of subcutaneous and epididymal fat depots averaged 170.5±15.5 p.p.m. and 171.7±14.7 p.p.m., respectively, and the differences between the two tissues were not significant (P=0.30). The 2H enrichments of subcutaneous and epididymal fat depots were highly correlated (r=0.89, P<0.001).
The proportion of synthesized de novo fatty acids accumulated in subcutaneous fat depots over 14 days averaged 27.1±8.5% for SD voles (N=12), 27.4±7.4% for LD voles during the increase phase (N=14) and 22.7±9.3% during the plateau phase (N=13). The contribution of de novo lipogenesis to fatty acids of epididymal fat depots in SD voles and LD voles during the increase and plateau phases averaged 27.1±8.3%, 27.3±6.6% and 24.7±9.9%, respectively. The fractional rates of de novo lipogenesis in subcutaneous and epididymal fat depots were not affected by photoperiod (Table 2). After pooling the data for all voles (N=39), the proportions of fatty acids synthesized from carbohydrates in subcutaneous (25.7±8.5%) and epididymal fat depots (26.4±8.2%) were not significantly different (P=0.30) and highly correlated within individuals (r=0.89, P<0.001).
Fatty acid composition
Comparison of total lipid fatty acid profile between adipose tissue of
voles exposed to different photoperiod regime was performed on fatty acids
that constituted >1% of all identified fatty acids. These included palmitic
(C16:0), palmitoleic (C16:1), stearic (C18:0), oleic (C18:1), linoleic (C18:2)
and linolenic (18:3) fatty acids.
The fatty acid composition of total lipids extracted from subcutaneous fat depots did not differ between SD voles and LD voles during the increase or plateau phases (Table 3). After pooling the data for all voles (N=9), the most abundant fatty acid in subcutaneous fat depots was oleic acid (36.2±2.9%), followed by linoleic (29.3±3.0%), palmitic (18.4±2.1%), palmitoleic (2.8±0.8%), stearic (1.4±0.3%) and linolenic (1.3±0.2%) acids.
|
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Discussion |
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The main strategy employed by adult voles to reduce winter maintenance
costs is a 20-40% reduction in body mass (e.g.
Iverson and Turner, 1974;
Hansson, 1992
;
Aars and Ims, 2002
). This
reduction affects all three major carcass components lean tissue, fat
and body water (Dark et al.,
1983
). A similar strategy is also adopted by immature voles born
late in the season, which delay growth to the adult size until the next spring
(e.g. Negus et al., 1986
;
Boonstra, 1989
;
Hansson, 1990
). Despite the
obvious benefits of this strategy (smaller animals need less food and
therefore spend more time in a warm and possible safe nest), reduced body mass
also has some disadvantages. These include a higher surface-to-volume ratio
and consequently a higher relative heat loss, a lower thermogenic capacity to
sustain body temperature during cold periods and a lower fasting endurance to
survive prolonged periods of negative energy balance (e.g.
Millar and Hickling, 1990
;
Speakman, 1996
;
Jackson et al., 2001
). To
counteract these problems, wintering voles build larger nests, increase the
insulative properties of their pelage and also rely on energetic benefits of
huddling (e.g. Walsberg, 1991
;
Hayes et al., 1992
).
In field vole populations, the mean wintering body mass may vary
considerably between animals at different locations and between years within
the same location (e.g. Chitty,
1952; Agrell et al.,
1992
). Individuals in increasing populations are usually larger
than those in declining populations (Krebs
and Myers, 1974
). Interestingly, when voles are moved among
locations that differ in mean wintering body mass, the transplanted
individuals adjust their body size to the average mass at the site to which
they are moved (Ergon et al.,
2001
). This suggests that the optimal wintering body mass depends
on extrinsic factors associated with the immediate environment (e.g. ambient
temperature, food quality/availability, predation or parasite load) rather
than intrinsic factors such as age distribution, physiological state or
genetic and maternal effects (Speakman et
al., 2003
; Ergon et al.,
2004
). Although a smaller body mass increases winter survival, a
larger wintering body mass may enable an earlier onset of reproduction
(Ergon et al., 2001
). In
response to this trade-off, voles have evolved the ability to switch between
winter- and summer-appropriate body masses by undergoing rapid spring growth,
which is initiated by increasing day length.
Although the rate at which field voles gained weight in our study (24.8%
increase over 4 weeks) was similar to that reported in Syrian hamsters (27.5%
increase over 4 weeks as recalculated from
Campbell and Tabor, 1983) and
collared lemmings (23.6% increase over 4 weeks as recalculated from
Nagy, 1993
), the mechanisms
behind the deposition and maintenance of this extra tissue appear to be
different. Both Syrian hamsters and collared lemmings exposed to SD increase
body mass without increasing food intake. This could be achieved by increasing
digestive efficiency and/or by metabolic compensation, i.e. diverting some
energy from maintenance to somatic growth and fat deposition. The fact that
neither Syrian hamsters nor collared lemmings increase digestive efficiency in
SD (Bartness and Wade, 1985
;
Nagy and Negus, 1993
) strongly
suggests that some aspect of their energy expenditure (such as basal
metabolism, thermoregulation or activity) must be reduced. Indeed, it has been
demonstrated that Syrian hamsters exposed to SD reduce wheel-running activity
(and presumably energy spent on locomotion) by approximately 66% (males) and
48% (females) relative to controls maintained in LD
(Ellis and Turek, 1979
;
Widmaier and Campbell, 1980
).
Furthermore, energy savings generated by winter communal nesting could also
contribute to an accelerated mass gain. Syrian hamsters housed in groups for
6575 days displayed a chronic 13% reduction in mass-specific resting
metabolic rate, accompanied by a 20% increase in body mass and a 53% increase
in body fatness, relative to individuals housed individually
(Borer et al., 1988
). Decrease
in maintenance metabolism has also been demonstrated in collared lemmings
(Powell et al., 2002
). In
particular, male lemmings exposed to SD for 10 days had significantly lower
total energy expenditure, resting energy expenditure and UCP-1 mRNA levels
than animals maintained in LD, whereas non-resting energy expenditure (related
to activity) was not affected by photoperiod. These data indicate that the
increase in body mass that occurs when collared lemmings are exposed to SD may
be primarily fuelled by a decrease in resting metabolic rate, related to the
differences in the activity of brown adipose tissue.
In contrast to Syrian hamsters and collared lemmings, field voles responded
to photoperiod-induced body mass gain by increasing their food intake (Figs
1B,
2A). Over the period of body
mass increase (days 128 of exposure to long day photoperiod), LD voles
ate 16.3 g more food than SD individuals. Over the next 4 weeks (days
2956), the difference between food intake of LD voles (8.1 g
day-1) and SD controls (7.0 g day-1) stabilized at 1.1 g
day-1. This strategy of increasing total energy intake instead of
reducing energy spent on maintenance may be related to the fact that
grass-eating voles have evolved a large and efficient digestive system
(McNab, 1986;
Koteja and Weiner, 1993
),
which enables them to assimilate larger amounts of energy when high-quality
grass is abundant. Conversely, the ability of collared lemmings to increase
food intake may be limited by the capacity of the liver to detoxify their
diet, which consists of dicotyledons such as Salix shrubs and
Dryas spp. (Batzli,
1993
; Negus and Berger,
1998
). Although dicotyledons as a food source are seasonally more
stable and more nutritious than grass, they also contain more plant secondary
compounds and are, therefore, potentially more toxic. Thus, the ability of
field voles to adjust their food intake to an increasing body mass may also
depend on the nutritional ecology of this species.
Field voles exposed to LD not only adjusted their food intake to the increasing body mass but also improved their apparent digestive efficiency, from 74.2% in SD conditions to 81.4% during the rapid body mass gain (days 128 of exposure to LD) and 80.3% during the final plateau phase (days 2956 of exposure to LD). Consequently, although mass-adjusted food intake did not differ between the photoperiod groups (Fig. 2A), the LD voles undergoing body mass increase (days 128) assimilated on average 8.4 kJ day-1 more than animals maintained in SD (Fig. 2B). This surplus of energy was presumably allocated toward somatic growth and deposition of fat.
This raises the question of whether 8.4 kJ day-1 covers all the
energetic demands associated with the body mass gain, or only part of them. If
the latter is the case, some energy that is allocated to growth would have to
be withdrawn from maintenance. Because we did not measure the energy costs of
deposition (i.e. the sum of the energy accumulated as new tissue and costs of
its biosynthesis), the answer to this question depends on which values,
estimated for other species, are assumed to apply to voles. Energy costs of
deposition vary from 16.5 kJ per 1 g of new tissue assimilated in rats
(Rattus norvegicus; Farrell and
Williams, 1989) to 20.9 kJ g-1 in hedgehogs
(Erinaceus concolor; Król,
1994
) and 35.9 kJ g-1 in common voles (Microtus
arvalis; Jagosz et al.,
1979
). If we assume the costs of deposition in field voles to be
16.5, 20.9 or 35.9 kJ g-1, LD voles that produced 7.5 g of tissue
over 28 days would have to allocate 4.4, 5.6 and 9.6 kJ day-1 more
to growth, respectively, than animals maintained in SD. Thus, depending on the
assumption, the difference in metabolizable energy intake between voles
exposed to LD for 128 days and SD controls (8.4 kJ day-1)
could meet 87 to 100% of the energy demands associated with the body mass
gain. After the increase in body mass was complete (days 2956), the
mass-adjusted metabolizable energy intake of LD voles did not return to the SD
level, but remained on average 7.6 kJ day-1 higher
(Fig. 2B). This may reflect
photoperiod-induced changes in the physiology and behaviour of male voles,
related to the need for an intense competition for polygynous territories in
the early spring (Ostfeld,
1985
). Additional studies on the energy costs of deposition and
other components of the energy budget are required to evaluate these
possibilities.
During the first 4 weeks of exposure to LD, field voles produced on average
2.0 g of white adipose tissue, which in turn led to an increase in body
fatness, from 7.1% in SD voles to 12.9% in voles exposed to LD for 2970
days (Table 1). The mechanisms
enabling mammals to deposit fat include elevated dietary lipid uptake and
increased de novo lipogenesis, i.e. conversion of dietary
carbohydrates to fatty acids. Therefore, if the voles accumulating adipose
tissue (days 128 of exposure to LD) were limited by the amount of
dietary fat available for synthesis of triacylglycerides, we would expect them
to increase de novo lipogenesis. Otherwise, the rate of fatty acid
synthesis should remain unchanged or decrease. The latter would suggest that
although voles had enough dietary fat to accumulate adipose tissue, they were
limited by the amount of energy available for somatic growth and fat
deposition. Conversion of carbohydrates to fat prior to ß-oxidation is
thermogenically costly and approximately 28% of the energy content of
carbohydrates is lost as heat (Flatt,
1978; Hellerstein,
1999
).
Over the period when voles accumulated 2.0 g of adipose tissue (days 128 of exposure to LD), they digested on average 6.7 g of dietary fat (6.9 g daily food intake x 3.4% crude oil in the diet x 28 days). The actual amount of dietary lipid available for fat deposition depends on partitioning of dietary fat for ß-oxidation versus storage and remains unknown. However, voles that were accumulating adipose tissue had proportions of de novo synthesized fatty acids in subcutaneous and epididymal fat depots (27.1 and 27.1%, respectively) similar to SD voles (27.4 and 27.3%, respectively) and LD voles during the plateau phase (22.7 and 24.7%, respectively) (Table 2). Thus, regardless of the photoperiod-induced differences in fat deposition and the type of adipose tissue, 7377% of the stored lipids had dietary origin. This suggests that the voles accumulating adipose tissue were not limited by the amount of dietary fat available for synthesis of triacylglycerides.
We also demonstrated that fatty acid composition of subcutaneous fat depots
in voles accumulating adipose tissue (days 128 of exposure to LD) was
not significantly different from that in SD voles or LD voles during the
plateau phase (Table 3). These
results suggest that there was no gross shift in the source of lipids
deposited in stores, which is consistent with the fact that that photoperiod
had no effect on de novo lipogenesis. The most abundant fatty acids
in vole adipose tissue were oleic (36.2%), linoleic (29.3%) and palmitic
(18.4%) acids. Similar results have been reported for other mammals, including
Norway rats Rattus norvegicus
(Reidinger et al., 1985), deer
mice Peromyscus maniculatus
(Geiser, 1991
), fat-tailed
dwarf lemurs Cheirogaleus medius
(Fietz et al., 2003
), echidnas
Tachyglossus aculeatus
(Falkenstein et al., 2001
) and
polar bears Ursus maritimus
(Grahl-Nielsen et al.,
2003
).
In conclusion, field voles may provide an attractive novel animal model for
investigation of the regulation of body mass and energy homeostasis at both
organism and molecular levels. When exposed to LD, cold-acclimated male field
voles showed an increase in body mass, which reflected significant increases
in absolute amounts of all body components, including dry fat mass, dry lean
mass and body water mass. To meet energetic demands of deposition and
maintenance of extra tissue, field voles adjusted their food intake to an
increasing body mass and improved their apparent digestive efficiency. On
average, 7377% of fat accumulated as adipose tissue had dietary origin.
The rate of de novo lipogenesis and fatty acid composition of adipose
tissue were not affected by photoperiod. The most important characteristics of
this new model are the clear delineation between phases where animals regulate
body mass at two different levels and the rate at which animals are able to
switch between different levels of energy homeostasis. Field voles maintained
in SD regulated their body mass at the lowest seasonal level. After 4 weeks of
exposure to LD they regulated body mass at a 24.8% higher level than prior to
exposure. By comparison, Siberian hamsters, Syrian hamsters and collared
lemmings restore homeostasis not earlier than 8 weeks following photoperiod
manipulation (e.g. Campbell and Tabor,
1983; Nagy, 1993
;
Mercer et al., 2000
).
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Acknowledgments |
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Footnotes |
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