Fast muscle function in the European eel (Anguilla anguilla L.) during aquatic and terrestrial locomotion
1 School of Biology, University of Leeds, Leeds LS2 9JT, UK and
2 Experimental Zoology Group, Department of Animal Sciences, Wageningen Institute of Animal Sciences, Wageningen University, The Netherlands
*Author for correspondence at present address: Department of Biology, 414 Mugar, Northeastern University, Boston, MA 02115, USA (e-mail: dellerby{at}lynx.neu.edu)
Accepted April 9, 2001
![]() |
Summary |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Key words: European eel, Anguilla anguilla, locomotion, muscle, power output, kinematics, swimming, crawling.
![]() |
Introduction |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Axially, the lateral musculature is divided into a series of myotomes. In fish, different muscle fibre types with distinct properties are usually arranged in discrete populations within each myotome. Sustained locomotion at low tailbeat frequencies is powered by slow-twitch aerobic muscle (e.g. Bone, 1966; Rayner and Keenan, 1967; Rome et al., 1992; Coughlin and Rome, 1999). Fast-twitch fibres are recruited during fast starts and bursts of swimming at a high tailbeat frequency (e.g. Rayner and Keenan, 1967; Johnston et al., 1977). Fast fibres are also recruited during terrestrial locomotion in eels (Gillis, 2000).
In some teleosts with a distinct caudal fin, there may be subtle changes in muscle function along the body axis during swimming (e.g. van Leeuwen et al., 1990; Altringham et al., 1993; Altringham and Ellerby, 1999). Caudal fast muscle may produce some negative work and have a role in power transmission as well as power production. Also, in most teleosts studied, muscle properties are known to change along the body: muscle kinetics slow down from head to tail, but the link between these observations remains unexplained (Altringham and Ellerby, 1999). It has been hypothesised that muscle properties and muscle function may be more homogeneous along the body of an eel than in fish with a distinct caudal fin. This is because thrust is thought to be produced more uniformly along the body axis rather than primarily in the caudal region (Wardle et al., 1995; DAoût and Aerts, 1999). The isometric properties of the fast muscle of the European eel Anguilla anguilla L. were measured at two points along the body axis to test for uniformity of muscle kinetics. To determine whether the caudal musculature produced any negative work that could be associated with a power-transmitting mechanism, the work loop technique (Josephson, 1985) was used to measure axial patterns of power production under approximated in vivo conditions.
Locomotion in a buoyant three-dimensional fluid medium probably requires different patterns of muscle function from locomotion on a two-dimensional solid surface. It has been shown that the body kinematics (Gillis, 1998a) and patterns of fast muscle activity (Gillis, 2000) in an eel are different between locomotion on land and in the water. We have also determined how the activation patterns associated with these two different modes of locomotion affect force production and power output in the fast muscle. Patterns of force and power production are discussed in relation to the differing demands of aquatic and terrestrial locomotion.
![]() |
Materials and methods |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
|
(Pankhurst, 1982), where A and B are horizontal and vertical eye diameters, respectively, and BL is body length (measurements in mm). Mean eye size index was 13.6±0.7 (mean ± S.E.M., N=7). This falls within the range reported previously (Ellerby et al., 2001) for A. anguilla yellow-phase eels. The fish were held in 2m diameter fibreglass tanks that contained aerated, filtered water at a temperature of 14°C. A 16h:8h light:dark photoperiod was maintained in the aquarium. The eels were fed a maintenance diet of freeze-dried bloodworms three times a week.
Eels were killed by decapitation, destruction of the brain and pithing of the spinal cord. Blocks of skeletal muscle tissue were removed from the lateral line region. These were placed in oxygenated Ringers solution (composition in mmoll-1: NaCl, 109; KCl, 2.7; NaHCO3, 2.5; CaCl2, 1.8; MgCl2, 0.47; sodium pyruvate, 5.3; Hepes, 10; pH7.4±0.05 at 14°C) cooled to 8°C. The tissue blocks were pinned out, and any slow muscle fibres, fat and skin were rapidly removed. The Ringers solution was replaced frequently during dissection. The resulting muscle preparations consisted of bundles of fast muscle fibres from the superficial anterior-pointing cones of the myotomes. These were approximately 0.3 mm2 in cross-sectional area and 34 mm in length and ran between two collagenous myosepta. They were connected directly to hooks on the servo arm and force transducer (AE801, SensoNor, Horten, Norway) via the connective tissue of the myosepta. Each muscle preparation was then allowed to recover for 1h. The preparations were bathed in recirculating, oxygenated Ringers solution at 14°C. The work loop apparatus and techniques were as described previously (e.g. Altringham and Johnston, 1990; Hammond et al., 1998).
Stimulus amplitude (2ms pulse) was adjusted to 120% of that giving a maximal response. The fibre length was then increased in increments of 0.2 mm using a micromanipulator until the preparation yielded a maximal twitch response. This was taken as resting length (l0) and corresponded closely to the resting fibre length in the fish when lying flat. A 2ms stimulus was used to compare the twitch kinetics of fibres from different axial locations. The activation time Ta, the time from stimulus onset to peak force, and T90, the time from stimulus onset to 90% relaxation, were measured. Tetanic stimulus frequency was adjusted to that yielding a maximum tetanic response (approximately 80Hz).
Muscle preparations were subjected to sinusoidal length changes. In vivo strain patterns in swimming fish are approximately sinusoidal (e.g. Hammond et al., 1998; Knower et al., 1999; Ellerby et al., 2000). Muscle preparations were subjected to strain cycles of ±5%l0. In vivo eel fast muscle strains are unknown. Calculated superficial strains ranged from approximately ±5 to ±12%l0 in A. anguilla during forward swimming and were as high as ±25%l0 during backward swimming (DAoût and Aerts, 1999). Because of fibre geometry and greater proximity to the backbone, fast muscle strains are thought to be lower than superficial slow muscle strains (Alexander, 1969).
Stimulus parameters are expressed in degrees relative to the 360° sinusoidal strain cycle where the muscle is at resting length l0 and lengthening at 0°. Approximated in vivo stimulation parameters were derived from Gillis (Gillis, 2000) (Table1). These data related to Anguilla rostrata not A. anguilla. These two Atlantic eel species cannot be unambiguously separated on the basis of morphology or enzyme electrophoresis (Williams and Koehn, 1984). There are small differences in mitochondrial DNA sequences (e.g. Avise et al., 1990) that separate the two species, but the difference between A. anguilla and A. rostrata is only slightly greater than the variation displayed within A. anguilla (Bastrop et al., 2000). On the basis of this high degree of similarity, it seemed reasonable to use these parameters to approximate the in vivo stimulus patterns of A. anguilla. The stimulus parameters required for maximum power production were obtained by systematically altering stimulus onset phase and duration relative to the strain cycle.
|
To detect any change in muscle performance during the experiment, preparations were subjected to a control trial, with fixed parameters (cycle frequency 2Hz, strain ±5%, stimulus onset 60° and stimulus duration 100°) after every fourth run. Power outputs were scaled relative to these control trials. After a small decrease in power output during the initial stages of the trial, power output stabilised and remained virtually constant for the remainder of the experiment (up to 6h in total).
Statistical analyses
Statistical analyses were carried out using SigmaStat (SPSS, Chicago, IL, USA) software. Basic muscle mechanical properties were tested for significant differences between body positions using a Students t-test. Power and work outputs were tested for significant differences between stimulus regimes using analysis of variance (ANOVA). Significant differences were measured using the StudentNewmanKeuls test. Values are given as means ± S.E.M.
![]() |
Results |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
|
|
|
|
|
|
|
![]() |
Discussion |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Maximum isometric tetanic stresses were within the range measured in other teleost fast muscle. Eggington (Eggington, 1987) measured a maximum isometric stress of 102kNm-2 in single fast fibres of the yellow-phase American eel, lower than that (140kNm-2) measured in the present study. Isometric tetanic stresses were comparable with those measured (Altringham et al., 1993) in the fast muscle of the saithe (Pollachius virens, 136151kNm-2).
Fast muscle power output
The maximum fast muscle power outputs of 1617Wkg-1 measured in the present study were lower than those measured in the fast muscle of other teleosts. Maximum fast muscle power output was 63Wkg-1 in the saithe (Pollachius virens) (Altringham et al., 1993) and 35Wkg-1 in the sculpin (Altringham and Johnston, 1990). The power requirements of swimming scale to approximately the third power of swimming speed (Webb, 1978). The low power output of eel fast muscle suggests that the maximum swimming speed must be low relative to that of these other teleosts. The only previous measurement of eel muscle power output was obtained using whole-body work loop experiments on the American eel (Long, 1998) in which the maximum power output measured was 23Wkg-1. The entire lateral musculature in the caudal region was stimulated at a cycle frequency of 3Hz. As the majority of the lateral musculature is made up of fast fibres, this was effectively a measure of fast muscle power output. The relative stimulus duration used by Long (180°; Long, 1998) was much greater than that measured in vivo (Gillis, 2000, Table1). Maximum power output was achieved at a stimulus onset of 325° relative to the strain cycle. The optimal stimulus onset/duration at 3Hz in isolated fibres in the present study was 50°/90°. In isolated muscle preparations, a stimulus onset of 325° early in the lengthening phase would result in a large negative work component during the strain cycle and a low net work output. Long (1998) suggested that this activation pattern stretched a series elastic component within the lateral musculature which returned energy later in the strain cycle. The disparity between the optimum stimulus onset for power output using isolated fibres and whole-body work loops highlights the fact that the interactions between the muscle fibres and their mechanical environment in the intact fish are complex and poorly understood.
Despite the longitudinal differences in isometric properties, there are no apparent differences with axial position in the optimal stimulation parameters required for maximum power output or the relationship between power output and cycle frequency. The cycle frequency yielding maximum power output (2Hz) was much lower in eel fast muscle than in the fast muscle of other fish. For example, peak frequency was 8Hz in the saithe (Altringham et al., 1993) and 57Hz in the sculpin (Altringham and Johnston, 1990). Power output is the product of cycle frequency and the work output per cycle. The low operating frequency of eel fast muscle accounts for its low power output relative to other teleosts. Work output provides a means for comparing fast muscle performance with that of other teleosts because it removes the influence of cycle frequency. At peak power output (cycle frequency 2Hz) under optimal stimulus conditions, the work output per cycle of eel fast muscle was 8.2Jkg-1 in the anterior muscle and 8.6Jkg-1 in the posterior muscle. The work output of saithe muscle at maximum power output was 7.9Jkg-1 (Altringham et al., 1993), and that of the sculpin was 7.0Jkg-1 (Altringham and Johnston, 1990). On this basis, the performance of eel fast muscle is comparable with that of other teleost fast muscle.
There was no substantial negative work or power component during the strain cycle (Fig.5). A small amount of negative work was associated with activation whilst lengthening under swimming stimulus conditions. A large negative work component has been measured or calculated during the strain cycle in the caudal muscle of some teleosts. It has been suggested that this may stiffen the caudal muscle during part of the strain cycle as a means of power transmission from more anterior muscle to the caudal fin (van Leeuwen et al., 1990; Altringham et al., 1993). The lack of substantial negative power and work production in eel caudal fast muscle suggests that there is no reliance on stiffened fast muscle for power transmission along the body axis. This is consistent with the view of relative uniformity of muscle function and limited requirements for axial power transmission in anguilliform swimmers where thrust is generated along much of the body axis rather than by a caudal fin (Wardle et al., 1995; Altringham and Ellerby, 1999).
Why is the operating frequency of eel fast muscle lower than that of other fish? The twitch activation times (Ta, 8293ms) were longer than those measured in the fast muscle of the saithe. Ta in the saithe ranged from 27 to 44ms (Altringham et al., 1993). Relaxation times were also relatively longer in eel fast muscle. T90 in the eel (203239ms) was also longer than that of the saithe (62136ms, Altringham et al., 1993). Relatively slow contraction times in eel fast muscle may impose an upper limit on the cycle frequency at which it can operate. In repeated length change cycles, failure of the preparation to relax fully before the subsequent cycle results in negative work production because stresses remain high during muscle lengthening.
Locomotory performance in water and on land
On the basis of in vitro patterns of power production at 14°C, European eels could not attain a tailbeat frequency greater than 4Hz during swimming. Direct comparisons with previous kinematic studies are complicated by temperature differences. In yellowfin tuna (Thunnus albacares) and bonito (Sarda chiliensis), a temperature rise from 15 to 20°C resulted in a doubling of the cycle frequency at which peak power production was achieved (Altringham and Block, 1997). The highest tailbeat frequencies recorded in eels at 20°C were approximately 4Hz in A. anguilla (DAoût and Aerts, 1999) and 3.3Hz in A. rostrata (Gillis, 1998b), although measuring the maximum possible tailbeat frequency was not the aim of these studies. If the temperature difference is accounted for, then at 20°C the optimal cycle frequency for power production may be closer to 4Hz. Grillner and Kashin (Grillner and Kashin, 1976) measured tailbeat frequencies of 5Hz in A. anguilla, beyond the frequency range achieved in vitro. The temperature at which this occurred was not stated and may well have been higher than in the present study.
The maximum cycle frequency at which positive power output could be achieved in vitro was much lower under simulated crawling conditions (2Hz). At cycle frequencies above 2Hz, because of the relatively late stimulus onsets and durations, the muscle could not relax fully before the subsequent strain cycle. These in vitro measurements are consistent with kinematic observations: the highest cycle frequency recorded by Gillis (Gillis, 1998a) for A. rostrata on land was 1.3Hz.
The stimulus parameters that maximised power production (Fig.1) were different from those measured in vivo for A. rostrata by Gillis (Gillis, 2000) (Table1). Generating maximum power output is not necessarily the only consideration in vivo. The conditions for maximum efficiency, for example, can differ from those that generate maximum power output (Curtin and Woledge, 1996). The different stimulus patterns associated with swimming and crawling (Table1) produced very different patterns of force and power production during the strain cycle (Fig.4 , Fig.5). This was reflected in the shapes of the resulting work loops (Fig.3). Higher peak stresses were generated by the activation patterns associated with swimming (Table3; Fig.4). This was because during simulated swimming the onset of muscle activity occurred in the lengthening phase just prior to maximum length. This type of activation pattern has been shown to enhance muscle force and power production during the subsequent shortening phase (e.g. Altringham and Johnston, 1990). The stimulus parameters required for maximum power output included onset of activation during lengthening (Fig.1). During simulated crawling, the onset of muscle activity occurred during shortening, and force production was consequently lower.
Below a cycle frequency of 1Hz, the stimulus patterns associated with crawling resulted in a greater power output in the posterior muscle than those associated with swimming (Fig.2). However, above 1Hz, power output was greater under simulated swimming conditions. In the anterior muscle, power output was always lower during simulated crawling than during simulated swimming. Why would a shift in stimulus patterns that in general results in an overall decrease in power and force production occur on transition to land? It is possible that locomotion on an unyielding surface may require the generation of less force than thrust generation in a fluid. However, fast muscle electromyographic intensity is greater on land than in water (Gillis, 2000). This suggests an increased recruitment of locomotor units that may, in part, offset the general reduction in fast muscle work and power output on land. Movement from an environment in which the body is no longer supported by buoyancy may also require greater force production. The greater degrees of body curvature and lateral displacement associated with crawling probably increase fast muscle strain. This may increase work and power production on land.
An explanation may lie in the shift in kinematics on transition to land. During swimming, not all parts of the body follow the same path: the amplitude envelope of the body increases from anterior to posterior (Gillis, 1998a; DAoût and Aerts, 1999; Ellerby et al., 2001). During terrestrial locomotion, all points on the body axis follow a similar path and when crawling on sand the body of the eel creates a groove in the substratum (Gillis, 2000). The posterior parts of the body follow the groove created by the anterior parts, and the groove may be used to improve traction. This is also the case during terrestrial crawling in snakes (Mosauer, 1932; Gray, 1946). If the kinematics remained the same as during swimming, then the eel would be unable to take advantage of this mechanism. The shift in activation patterns and the resultant changes in force production may be related to this kinematic shift, making the associated loss in performance unavoidable if traction is to be maintained. It would be interesting to determine how different types of surface affect terrestrial kinematics and whether more solid surfaces in which a groove could not be produced would result in a different pattern of muscle function.
![]() |
Acknowledgments |
---|
![]() |
References |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Alexander, R. McN. (1969). The orientation of muscle fibres in the myomeres of fish. J. Mar. Biol. Ass. U.K. 49, 263290.
Altringham, J. D. and Block, B. A. (1997). Why do tuna maintain elevated slow muscle temperatures? Power output of muscle isolated from endothermic and ectothermic fish. J. Exp. Biol. 200, 26172627.
Altringham, J. D. and Ellerby, D. J. (1999). Fish swimming: patterns in muscle function. J. Exp. Biol. 202, 33973403.
Altringham, J. D. and Johnston, I. A. (1990). Modelling muscle power output in a swimming fish. J. Exp. Biol. 148, 395402.
Altringham, J. D., Wardle, C. S. and Smith, C. I. (1993). Myotomal muscle function at different locations in the body of a swimming fish. J. Exp. Biol. 182, 191206.
Avise, J. C., Nelson, W. S., Arnold, J., Koehn, R. K., Williams, G. C. and Thorsteinsson, V. (1990). The evolutionary genetic status of Icelandic eels. Evolution 44, 12541262.
Bastrop, R., Strehlow, B., Jürss, K. and Sturmbauer, C. (2000). A new molecular phylogenetic hypothesis for the evolution of freshwater eels. Mol. Phylogenet. Evol. 14, 250258.[Medline]
Bone, Q. (1966). On the function of the two types of myotomal muscle fibre in elasmobranch fish. J. Mar. Biol. Ass. U.K. 46, 321349.
Coughlin, D. J. and Rome, L. C. (1999). Muscle activity in steady swimming scup, Stenotomus chrysops, varies with fiber type and body position. Biophys. J. 196, 145152.
Curtin, N. A. and Woledge, R. C. (1996). Power at the expense of efficiency in the contraction of white muscle from dogfish Scyliorhinus canicula. J. Exp. Biol. 199, 593601.
DAoût, K. and Aerts, P. (1999). A kinematic comparison of forward and backward swimming in the eel Anguilla anguilla. J. Exp. Biol. 202, 15111521.
Davies, M. L. F., Johnston, I. A. and Vandewal, J. (1995). Muscle-fibres in rostral and caudal myotomes of the Atlantic cod (Gadus morhua, L.) have different mechanical properties. Physiol. Zool. 68, 673697.
Eggington, S. (1987). Metamorphosis of the American Eel, Anguilla rostrata LeSeur. III. Contractile characteristics of skeletal muscle. J. Exp. Zool. 243, 3950.[Medline]
Ellerby, D. J., Altringham, J. D., Williams, T. and Block, B. A. (2000). Slow muscle function of Pacific bonito (Sarda chiliensis) during steady swimming. J. Exp. Biol. 203, 20012013.
Ellerby, D. J., Spierts, I. L. Y. and Altringham, J. D. (2001). Slow muscle power output of yellow- and silver-phase European eels (Anguilla anguilla, L.): changes in muscle performance prior to migration. J. Exp. Biol. 204, 13691379.
Gillis, G. B. (1998a). Environmental effects on undulatory locomotion in the American eel Anguilla rostrata: kinematics in water and on land. J. Exp. Biol. 201, 949961.
Gillis, G. B. (1998b). Neuromuscular control of anguilliform locomotion: patterns of red and white muscle activity during swimming in the American eel Anguilla rostrata. J. Exp. Biol. 201, 32453256.
Gillis, G. B. (2000). Patterns of white muscle activity during terrestrial locomotion in the American eel (Anguilla rostrata). J. Exp. Biol. 203, 471480.
Gray, J. (1946). The mechanism of locomotion in snakes. J. Exp. Biol, 23, 101120.
Gray, J. (1968). Animal Locomotion. New York: W. W. Norton Co. Inc.
Grillner, S. and Kashin, S. (1976). On the generation and performance of swimming in fish. In Neural Control of Locomotion (ed. R. M. Herman, S. Grillner, P. S. G. Stein and D. G. Stuart), pp. 181201. New York: Plenum.
Hammond, L., Altringham, J. D. and Wardle, C. S. (1998). Myotomal slow muscle function of rainbow trout Oncorhynchus mykiss during steady swimming. J. Exp. Biol. 201, 16591671.
Jayne, B. C. and Lauder, G. V. (1995). Are muscle fibers within fish myotomes activated synchronously? Patterns of recruitment within deep myomeric musculature during swimming in largemouth bass. J. Exp. Biol. 198, 805815.
Johnson, T. P., Syme, D. A., Jayne, B. C., Lauder, G. V. and Bennett, A. F. (1994). Modeling red muscle power output during steady and unsteady swimming in largemouth bass. Am. J. Physiol. 267, 418488.
Johnston, I. A., Davison, W. and Goldspink, G. (1977). Energy metabolism of carp swimming muscles. J. Comp. Physiol. 114, 203216.
Johnston, I. A., Franklin, C. E. and Johnson, T. P. (1993). Recruitment patterns and contractile properties of fast fibres isolated from rostral and caudal myotomes of the short horned sculpin J. Exp. Biol. 185, 251265.
Josephson, R. K. (1985). Mechanical power output from striated muscle during cyclic contractions. J. Exp. Biol. 114, 493512.
Knower, T., Shadwick, R. E., Katz, S. L., Graham, J. B. and Wardle, C. S. (1999). Red muscle activation patterns in yellowfin (Thunnus albacares) and skipjack (Katsuwonus pelamis) tunas during steady swimming. J. Exp. Biol. 202, 21272138.
Lindsey, C. C. (1978). Form, function and the locomotory habits in fish. In Fish Physiology, vol. VII (ed. W. S. Hoar and D. J. Randall), pp. 1100. New York: Academic Press.
Long, J. H. (1998). Muscles, elastic energy and the dynamics of body stiffness in swimming eels. Am. Zool. 38, 771792.
Mosauer, W. (1932). On the locomotion of snakes. Science 76, 583585.
Pankhurst, N. W. (1982). Changes in body musculature with sexual maturation in the European eel Anguilla anguilla (L.). J. Fish Biol. 21, 417428.
Rayner, M. D. and Keenan, M. J. (1967). Role of red and white muscles in the swimming of the skipjack tuna. Nature 214, 392393.[Medline]
Rome, L. C., Choi, I., Lutz, G. and Sosnicki, A. (1992). The influence of temperature on muscle function in the fast swimming scup. I. Shortening velocity and muscle recruitment during swimming. J. Exp. Biol. 163, 259279.[Abstract]
Rome, L. C., Swank, D. and Corda, D. (1993). How fish power swimming. Science 261, 340343.[Medline]
van Leeuwen, J. L., Lankheet, M. J. M., Akster, H. A. and Osse, J. W. M. (1990). Function of red axial muscles in carp (Cyprinus carpio L.): recruitment and normalised power output during swimming in different modes. J. Zool., Lond. 220, 123145.
Wardle, C. S. and Videler, J. J. (1993). The timing of the electromyogram in the lateral myotomes of mackerel and saithe at different swimming speeds. J. Fish Biol. 42, 347359.
Wardle, C. S., Videler, J. J. and Altringham, J. D. (1995). Tuning in to fish swimming waves: body form, swimming mode and muscle function. J. Exp. Biol. 198, 16291636.
Wardle, C. S., Videler, J. J., Arimoto, T., Franco, J. M. and He, P. (1989). The muscle twitch and the maximum swimming speed of giant bluefin tuna, Thunnus thynnus L. J. Fish Biol. 35, 129137.
Webb, P. W. (1978). Hydrodynamics: Nonscombroid fish. In Fish Physiology, vol. VII (ed. W. S. Hoar and D. J. Randall), pp. 190239. New York: Academic Press.
Williams, G. C. and Koehn, R. K. (1984). Population genetics of North Atlantic catadromous eels (Anguilla). In Evolutionary Genetics of Fishes (ed. B. J. Turner), pp. 529560. New York: Plenum.
Williams, T. L., Grillner, S., Smoljaninov, V. V., Wallen, P., Kashin, S. and Rossignol, S. (1989). Locomotion in lamprey and trout: the relative timing of activation and movement. J. Exp. Biol. 143, 559566.