Cn11, the first example of a scorpion toxin that is a true blocker of Na+ currents in crayfish neurons
1 Department of Molecular Recognition and Structural Biology, Biotechnology Institute, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Cuernavaca 62210, México,
2 Department of Physiology, Biophysics and Neurosciences, Centro de Investigación y de Estudios Avanzados del I.P.N. México DF 07000 and
3 Division of Graduate Studies and Research, Medical School, Universidad Nacional Autónoma de México, Ciudad Universitaria, México DF 04510
*Author for correspondence (e-mail: possani{at}ibt.unam.mx)
Accepted 4 January 2002
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Summary |
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Key words: amino acid sequence, Centruroides noxius, crayfish, neuron, Na+ channel, Procambarus clarkii, scorpion toxin.
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Introduction |
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Tetrodotoxin (TTX) and saxitoxin (STX) are small molecules with one and two guanidinium groups, respectively, and are known to block Na+ currents by interacting with the outer entrance of the ion channel pathway. Since TTX and STX are small molecules, it is conceivable that their binding site within the channel protein is very close to the narrowest part of the pore, which is believed to be the selectivity filter (Terlau and Stühmer, 1998). These neurotoxins bind to receptor site 1 of the Na+ channel, where the blocker toxins interact (Catterall, 1996
). To date, the only peptide described as a blocker of Na+ channels is µ-conotoxin GIII, a 22-amino-acid peptide isolated from the venom of the snail Conus geographus. This toxin acts in a similar manner to TTX and STX (McIntosh et al., 1999
; Dudley et al., 1995
).
Apart from the functional classification, there is also specificity in the toxins affecting Na+ channels, in either mammals or arthropods (mostly insects and/or crustaceans). However, binding studies, correlated with toxicity towards mammals and insects, have revealed that even toxins known as mammal-specific can be toxic to insects and vice versa (Gordon et al., 1996). Therefore, the animal group specificity is only relative, and definite cross-reactivity exists (Selisko et al., 1996
).
Scorpion toxins are classified according to their structure, mode of action and binding site on different channels or channel subtypes (Martin-Eauclaire and Couraud, 1995; Gordon et al., 1996
). Each class consists of several peptides isolated from the venom of different species of scorpion. The long-chain toxins affecting Na+ channels have been subdivided primarily into two major sub-types,
- and ß-toxins (Jover et al., 1980
; Wheeler et al., 1983
). The
-toxins bind to receptor site 3 of the voltage-gated Na+ channels of vertebrates in a membrane-dependent manner (Catterall, 1986
). For these toxins, Tugarinov et al. (1997
) and Zilberberg et al. (1992
) have described the area of the surface (active site) of the toxin that is capable of interacting directly with the receptor. The ß-toxins, initially isolated from American scorpions, bind to receptor site 4 on vertebrate Na+ channels, producing a shift to a more negative potential. Binding is independent of the membrane potential (Catterall, 1986
; Couraud and Jover, 1984
).
Two other distinct classes of toxin showing specificity for arthropods, termed depressant and excitatory, have been described (Zlotkin, 1987). These four classes of long-chain toxin share only 2040 % similarity of amino acid sequences (Dufton and Rochat, 1984
; Martin-Eauclaire and Couraud, 1995
).
Recently, Possani et al. (1999a) proposed a new classification, comprising 10 different groups, on the basis of structural and physiological effects and species-specificity. Toxins specific for mammals, insects and crustaceans and toxins with more than one specificity were placed in distinct groups. Below, we show that the toxin described here does not fit into any of the previously described groups.
The molecular basis of toxin specificity has been widely studied for various reasons: (i) to understand the toxic effects of scorpion venom as a pre-requisite for the development of more effective and safer antidotes or vaccines; (ii) to study ion channels, the target molecules of scorpion toxins, in order to understand their molecular structure and function; and (iii) to develop insect-specific toxins that can be used as insecticides. However, the existing information is still limited. Thus far, small differences, i.e. single amino acid modifications within or near the critical binding region between the toxin and its receptor, have been considered. Also, a significant number of natural ligands that recognize these receptor molecules are still to be identified and studied from the venom of scorpions, among other organisms (Possani et al., 1999a).
This paper describes the mechanism of action of a new Na+ channel toxin, relatively specific for crustaceans. Cn11 blocks the Na+ current in cultured neurons from the X organsinus gland of crayfish without modifying the gating mechanism, and it is the first example of a new group (the eleventh) of scorpion toxins with such a molecular effect.
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Materials and methods |
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Purification procedures and lethality tests
Gel-filtration chromatography on Sephadex G-50 columns was used as a first step to separate the components, followed by ion-exchange chromatography (Possani et al., 1981). Further purification of subfractions by high-performance liquid chromatography (HPLC) was conducted as described previously (Selisko et al., 1996
; García et al., 1997
; Possani et al., 1999a
). Briefly, 100 mg of soluble venom was applied to a Sephadex G-50 column (0.9 cmx200 cm) in the presence of 20 mmol l1 ammonium acetate, pH 4.7, at a flow rate of 20 ml h1. Toxic fractions were tested in animal models (mice, crickets and crayfish) and further separated on a carboxymethyl cellulose column (CM-Cellulose; 0.9 cmx30 cm) equilibrated and developed in the presence of 20 mmol l1 ammonium acetate buffer, pH 4.7, with a continuous salt gradient from 0 mol l1 to 0.5 mol l1 NaCl. The third purification step was conducted by HPLC, with a Waters (Millipore Co., Milford, MA, USA) system, using a C4 or a C18 reverse-phase column (Vydac, Hisperia, CA, USA).
For lethality tests, three designations were used: non-toxic means that the animals showed no symptoms of intoxication within 24 h after injection, and the effect was similar to that of injecting phosphate-buffered saline or water alone. The toxic effect consisted of a host of symptoms such as salivation, lacrimation, dyspnoea, temporary paralysis of the limbs (mice) or paralysis (crickets and crayfishes). The toxic effect was termed lethal if at least one of the test animals died after injection.
Determination of the primary structure of Cn11
The amino acid sequence of the toxin (SWISS-PROT accession no. P58296) was obtained (i) by direct sequencing of the native toxin, (ii) by sequencing the reduced and carboxymethylated toxin (RC-toxin) and (iii) by sequencing fragments generated by enzymatic cleavage of RC-toxin. Reduction of the toxin with dithiothreitol, alkylation with iodoacetic acid, enzymatic cleavage with trypsin, chymotrypsin and endoproteinase V8 and separation by HPLC of the sub-peptides were performed as described previously (Possani et al., 1999a). Microsequence determination was carried out by automatic Edman degradation using either a Millipore apparatus (ProSequencer model 6600) or a Beckman (LF 3000 Protein Sequencer) machine, as described previously (García et al., 1997
). For the most C-terminal amino acid residues, mass spectrometry analysis (Scaloni et al., 2000
) or a new strategy developed in our laboratory (G. B. Gurrola, T. Olamendi-Portugal, C. Balderas and L. D. Possani, unpublished observations) was used. For the latter, a synthetic pentapeptide containing the sequence N-acetyl-Lys-Cys-Lys-Tyr-Lys was prepared to make a disulphide bridge with the terminal cysteine residue of the unknown peptide for sequencing. Since the N-terminal lysine residue of the synthetic peptide was blocked, only the unknown peptide containing a free thiol group would be sequenced, after preparing a heterodimer by mixed disulphide bridge formation, with the synthetic carrier pentapeptide.
Neuron culture techniques
Adult crayfish, Procambarus clarkii, were collected from Río Conchos, Chihuahua, México, and adapted to laboratory conditions for 1 week, under a 12 h:12 h light:dark photoperiod. Eyestalks were excised and placed in chilled crayfish saline solution (in mmol l1): 205 NaCl, 5.4 KCl, 13.5 CaCl2, 2.6 MgCl2 and 10 Hepes, adjusted to pH 7.4 with NaOH. The exoskeleton, muscles and connective tissue surrounding the neural structures were carefully removed under a dissecting microscope to expose the neuronal somata. Isolated X organ neurons were incubated in collagenase/dispase (200 µl ml1, Boëhringer Mannheim) dissolved in modified Leibovitz L-15 (Gibco) culture medium for 60 min. The enzyme was washed out, and the X organ neurons were dissociated by gentle suction through fire-polished micropipettes, as described previously (García et al., 1990), and plated onto a 200 µl recording chamber precoated with Concanavalin A (Type III, Sigma). The ionic composition of the culture medium was increased to match that of the crayfish saline solution. In addition, 5.5 mmol l1 glucose, 2 mmol l1 L-glutamine, 16 µg ml1 gentamycin (Shering Plough) and 5 µg ml1 streptomycin (Sigma) were added. Cultured cells were kept in darkness for 24 h before the experiments were conducted.
Electrophysiology
Voltage-clamp experiments in the whole-cell configuration were performed using cultured X organ neurons. Na+ current recordings were obtained using an Axopatch-200A amplifier (Axon Instruments). Pipettes were constructed from borosilicate capillaries (Sutter Instruments; CA, USA) using a horizontal puller (P-87 Flaming Brown, Sutter Instruments, USA). Pipettes were filled with a solution containing (in mmol l1): 20 NaCl, 2 CaCl2, 2 MgCl2, 210 CsCH3SO4, 5 EGTA-K and 10 Hepes, adjusted to pH 7.4 with CsOH. After being filled, the pipette tip resistance was 3.3±0.2 M. Series resistance was compensated at 6580 %. Na+ currents were filtered at a frequency of 5 kHz and stored on hard disk using the DigiData 1200 hardware acquisition system and its software (pClamp6, Axon Instruments, USA). Transient capacitative and leak currents were subtracted using the P/4 protocol.
To isolate the Na+ current, the neurons were superfused in a solution containing (in mmol l1): 190 NaCl, 5 KCl, 10 CaCl2, 2 MgCl2, 20 tetraethylamonium chloride (TEA-Cl), 5 4-amino pyridine (4-AP), 2 CdCl2 and 10 Hepes at pH 7.4. The toxin samples were kept lyophilized and freshly prepared for each experiment by dissolving in saline solution at the desired concentration and using the solution immediately. The solution containing the toxin (10 ml) was continuously superfused in the experimental chamber (200 µl). The toxin assays were made in neurons with stable Na+ currents.
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Results |
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In Fig. 2, we summarize the results necessary to align the complete amino acid sequence of this novel crustacean toxin, termed Cn11 (Cn from the abbreviation for the species Centruroides noxius and 11 because it was the eleventh peptide specific for Na+ channels purified to homogeneity from this venom).
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To complete the full sequence, two situations were also considered; the first concerns the peptide Tryp1, whose sequence starts with Thr at position 39, indicating that this enzyme had some residual chemotryptic activity contaminant because it cleaved at Tyr38. The second observation is that the second tryptic peptide, from residue Val55, gave unequivocal identification only up to residue Asn61. The molecular mass, experimentally determined to be 6972 Da, suggested that two additional residues were lacking, compatible with the presence of a Thr and a Cys. The latter was needed to complete the four disulphide bridges, since one cysteine was still missing. However, the exact positions of the missing Thr and Cys were not known. For this reason, a pentapeptide (described in Materials and methods) was synthesized and used to make a mixed disulphide heterodimer with Tryp2. The microsequencer results showed unequivocally that the amino acid in position 62 was Thr, leaving a cysteine at position 63. The theoretical relative molecular mass calculated for native Cn11 was 6973, close to the value determined experimentally. Thus, Cn11 is a peptide that contains 63 amino acid residues, folded compactly by four disulphide bridges, similar to other Na+-channel-specific scorpion toxins (for a review, see Possani et al., 1999a).
Effects of Cn11 toxin on crayfish neuronal Na+ channels
To obtain better voltage-clamp control, the effect of Cn11 on the Na+ current was tested in neurons after 24 h in culture; at this time, the cells show discrete axonal regeneration, but the mean current amplitude at 0 mV was approximately 2 nA.
Fig. 3A shows a set of superimposed traces of inward Na+ currents obtained before and during Cn11 toxin (1 µmol l1) superfusion evoked in response to 0 mV depolarising voltage steps applied every 15 s from a holding potential of 60 mV. The first three traces under control conditions were averaged (lowest trace, labelled control). In the presence of the toxin, the current amplitude was gradually reduced, the maximum blocking effect being reached 3.5 min later (75 % reduction in current amplitude). This effect was only partially reversible (not shown).
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The effect of Cn11 is time- and concentration-dependent
To explore the concentration-dependence and the time course of the blockade induced by Cn11, the Na+ current amplitude was monitored every 10 s using the pulse protocol described in Fig. 3A. Three current traces were obtained under control conditions; the cells were then superfused with the same external solution but with Cn11 added for 160 s. The toxin was then washed from the recording chamber and the current was recorded for a further 160 ms. As is shown in Fig. 4A, the current amplitude was normalized with respect to the mean value obtained under control conditions. In the absence of the toxin, the Na+ current decreased by 10 % during the first 160 s; this rundown was taken into account in the subsequent estimation of the Na+ current amplitude in the presence of the toxin. After removal of the toxin from the recording chamber, the Na+ current recovered slowly for the lowest concentration tested (50 nmol l1).
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Cn11 does not modify the voltage-dependent activity of the neuronal Na+ channels
The voltage-dependence of the blockage exerted by Cn11 on the Na+ current was explored using command pulses to voltages between 50 and 50 mV from a holding potential of 60 mV. Under control conditions, the apparent activation threshold of the current was always 40 mV, and the maximal current was consistently near 0 mV (Fig. 5, open circles). During superfusion of 1 µmol l1 (filled squares) or 3 µmol l1 (open squares) Cn11, neither the apparent activation threshold nor the potential giving the maximal current was modified after 3 min of exposure to toxin, suggesting that the mechanism of action of Cn11 is quite similar to those of TTX and STX.
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Discussion |
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Several cleavages with different enzymes followed by HPLC separation of the corresponding fragments and individual sequencing were required to obtain the full amino acid sequence. The last two residues were identified only by using a synthetic carrier pentapetide, as described in Materials and methods.
Before analyzing in detail the results presented here, it is important to discuss briefly the current state of knowledge of Na+ channel blockade and permeability properties in the presence of different ligands including scorpion toxins. The specificity of Cn11 for the Na+ channel was assayed using cultured neurons from the crayfish eyestalk. As mentioned above, Cn11 did not affect the kinetic properties of isolated Ca2+ currents or outward K+ currents. Suprisingly, a small Na+ current (515 %) remained after blockade by Cn11 at high concentration and for a prolonged incubation time, suggesting that more than one type of Na+ channel is present in these cells. In a previous communication (Onetti et al., 1990), we showed that, there are at least two types of Na+ channel in these cells, one sensitive and the other insensitive to TTX. Assuming that this is the case, Cn11, like TTX, can discriminate between these two types of Na+ current.
Voltage-gated Na+ channels are the molecular target for a broad range of neurotoxins that act at six or more distinct receptor sites on the channel protein. There are hydrophilic polypeptides, of either low or high molecular mass, that physically block the pore and prevent the conductance of Na+. There also exist alkaloid toxins and related lipid-soluble toxins that alter the voltage-dependent gating of Na+ channels via an allosteric mechanism by binding to intramembranous receptor sites. In contrast, polypeptide toxins alter channel gating by voltage sensor trapping through binding to extracellular receptor sites. Studies defining the receptor sites and mechanisms of action of these diverse toxins were recently reviewed by Cestele and Catterall (2000).
If we analyze this review and that of Possani et al. (1999a), it is clear that none of the scorpion toxins studied thus far has a mechanism of action identical to that described here for Cn11. In Fig. 6, we show a comparative analysis of the identity found when comparing representative examples of each of the 10 proposed groups (Possani et al., 1999a
). Pairwise comparisons with Cn11 were made separately between each of the 10 groups described. Gaps were introduced to enhance similarities and to place cysteine residues in equivalent positions. Only group 10, which is an insect toxin (AaHIT), has a disulphide pairing that does not fit exactly with the others, and it has been reported that the disulphide arrangement of this toxin is different from that of the others (for reviews, see Gordon et al., 1998
; Possani et al., 1999a
). The most closely related group (group 4) has only 56 % identity with Cn11. All the other toxins have identities ranging from only 28 to 46 % (see Fig. 6). The two consensus (signature) sequences CXXXC and CXC, where C stands for cysteine and X for any residue, that serve to stabilize the three-dimensional structure by linking the
-helix stretch with the ß-sheet segments are conserved, except in AaHIT, as discussed. However, the most important argument in classifying Cn11 as a distinct group resides in its function. Cn11 blocks the Na+ currents of crayfish neurons in culture without apparently affecting the gating mechanism of the channels, an effect known to occur for the other groups of scorpion toxins.
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Cn11 is quite specific for crustaceans, having no detectable effect on mammals at the relatively high concentration used (more than 500 µg per 20 g mouse) (S. F. Serrano-Hernández and T. Olamendi-Portugal, unpublished). Cn11 could be a valuable tool in the investigation of the shape and structure of the outer vestibule of Na+ channels, as has been reported for other systems (Frech and Dudley, 1999; Marban et al., 1998
; Chahaine et al., 1998
; Dudley et al., 1995
; Li et al., 1997
; Fozzard and Lipkind, 1996
).
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Acknowledgments |
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