The molecular basis for spectral tuning of rod visual pigments in deep-sea fish
1 Departments of Molecular Genetics and
2 Visual Science, Institute of Ophthalmology, University College London, Bath Street, London, EC1V 9EL, UK and
3 School of Biological Sciences, University of Bristol, Bristol, BS8 1UG, UK
*Author for correspondence (e-mail: d.hunt{at}ucl.ac.uk)
Accepted July 2, 2001
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Summary |
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Key words: opsin, visual pigment, rod photoreceptor, deep-sea fish, spectral tuning, evolution.
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Introduction |
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Vertebrate visual pigments are composed of an opsin protein of approx. 350 amino acid residues that forms seven -helical transmembrane regions connected by cytoplasmic and luminal loops (Dratz and Hargrave, 1983; Findlay and Pappin, 1986), covalently attached via a protonated Schiff-base linkage to a chromophore. Each visual pigment shows a peak of maximal absorbance (
max), the precise location depending on interactions between the chromophore and specific amino acid residues of the opsin protein. In a previous study of visual pigments in four species of deep-sea fish with rod-only retinas containing visual pigments with
max values ranging from 483 nm to 468 nm (Hope et al., 1997), a number of candidate amino acid substitutions for spectral tuning were identified. However, the small number of species studied precluded a more detailed analysis of the mechanism of spectral tuning. A feature of deep-sea fish rod pigments is that their
max values tend to cluster at particular points in the spectrum rather than forming a continuous distribution (Bridges, 1965; Dartnall and Lythgoe, 1965; Partridge et al., 1989). The molecular basis for this phenomenon is unknown but could clearly be dependent on a common set of amino acid replacements, where each discrete shift in
max is achieved in all species by the same amino acid substitution.
In order to examine this and to determine whether the mechanism of spectral tuning proposed by Hope et al. (Hope et al., 1997) is of general applicability to the rod visual pigments of deep-sea fish, we have now extended the analysis to a much larger group of species drawn from seven different Orders of the Euteleostei, the Aulopiformes, Beryciformes, Gadiformes, Myctophiformes, Ophidiiformes, Osmeriformes and Stomiiformes. With only two exceptions, all the species studied possess only a single rhodopsin pigment in the retina (Ali and Anctil, 1976; Fröhlich et al., 1995; Partridge et al., 1988; Partridge et al., 1989; Douglas et al., 1995), indicating that only a single rod opsin gene is expressed in the photoreceptors. The exceptions are two species of dragon fish, Aristostomias tittmanni and Malacosteus niger. These species emit bioluminescent light with maxima beyond 700 nm (Widder et al., 1984), in addition to the more usual blue light. M. niger possesses a rhodopsin/porphyropsin pigment pair with max values of 517 nm and 550 nm, respectively. These pigments are based on a single rod opsin gene but with retinal or 3,4-dehydroretinal, respectively, as chromophore (Bowmaker et al., 1988; Douglas et al., 1998b). In addition, however, M. niger uses a remarkable photosensitizer based on a mixture of defarnesylated and demetallated derivatives of bacteriochlorophylls c and d in the retina to enhance the sensitivity of the pigment pair to its own longwave (LW) radiation (Bowmaker et al., 1988; Douglas et al., 1999a). The closely related species, A. tittmanni, lacks the photosensitizer. Instead, as well as possessing a rhodopsin/porphyropsin pigment pair with
max values of 523 nm and 551 nm, it has a third pigment with a
max of 581 nm, based most probably on a second opsin protein.
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Materials and methods |
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Sequencing of the rod opsin gene
The region of the rod opsin gene that encodes the seven -helices and associated cytoplasmic and luminal loops was amplified by polymerase chain reaction (PCR) and sequenced. Genomic DNA was extracted from liver or whole body samples by a standard phenol/chloroform extraction protocol. The first-round PCR amplifications utilised an oligonucleotide primer pair Frho91F (5'-CATATGAATACCCTCAGTACTACC-3') and Frho956R (5'-CCATTACCCATGTAAATGCAATTCCTG-3') that amplifies a fragment from nucleotides 91 to 956. Where an amplified product was not visible on an agarose gel, a second nested PCR was carried out using primer pair Frho173F (5'-TGTAAAACGACGGCCAGTCTTCCCYRTCAACTTCCTCAC-3') and Frho913R (5'-CAGGAAACAGCTATGACCTGCTTGTTCAWGCAGATGTAG-3'). The 5' region of the gene was amplified using primer pair Frho16F (5'-WWWWWATGAACGGYACRGAGG-3') and Frho229R (5'-AGAGGTYRGCMACNGCCAGGTTSAG-3').
Each PCR contained approximately 100 ng of template DNA, 12.5 µmol l1 of each primer, 0.2 mmol l1 each of dATP, dCTP, dGTP and dTTP, 4 mmol l1 Mg2Cl, 0.5 unit of Taq polymerase and 5 µl of reaction buffer in a final volume of 50 µl. Following an initial denaturation for 3 min at 94°C, 35 cycles were used with an annealing temperature of 56°C, an elongation temperature of 72°C and a denaturing temperature of 94°C. PCR products were passed through a Centricon 100 column (Princeton Scientific, Inc.) prior to direct sequencing with the Prism FS dye-deoxy Taq terminator kit, and the Prism Dyeprimer FS M13 forward and reverse kits. An ABI Model 373a sequencer was employed to generate the sequence. For each specimen, at least three independent PCR fragments were sequenced.
The rod opsin cDNA sequence was obtained for one species, Gonostoma elongatum. mRNA was isolated from eye tissue using the QuickPrep mRNA purification kit (Pharmacia) and cDNA synthesised using Superscript II reverse transcriptase, RNase H, and oligo-dT primer. The 5' and 3' ends of the coding sequence were then amplified by the RACE system (Gibco BRL) and the resulting products sequenced as described above.
Phylogenetic analysis
Neighbour-joining (Saitou and Nei, 1987) was used to construct a phylogenetic tree from the opsin gene sequences. The degree of support for internal branching was assessed by bootstrapping with 500 replicates. All computations were carried out with the MEGA computer package (Kumar et al., 1993).
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Results |
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Candidate sites for spectral tuning
Except for species from the same genus, the amino acid divergence between the rod opsin sequences of these deep-sea fish species is generally >20 %, reflecting the diversity of species examined in this study. In order therefore to identify candidate sites for spectral tuning, two overlapping approaches were used. The sequences were mapped on to a model based on conserved residues across >500 G-protein-linked receptor proteins (Baldwin, 1993; Baldwin et al., 1997). This model not only identifies the seven helices but, together with the three-dimensional map of frog rhodopsin determined by electron cryo-microscopy (Schertler and Hargrave, 1995), also provides a framework for orientating each helix with respect to the exterior lipid membrane and the central hydrophilic retinal-binding pocket. Only substitutions that result in a change in either charge or polarity (Nathans, 1990; Nakayama and Khorana, 1991) in residues that are located in the transmembrane helical regions and are either adjacent to this pocket or face another helix appear to be important for the spectral tuning of the resulting pigment (Merbs and Nathans, 1993; Asenjo et al., 1994; Hope et al., 1997; Hunt et al., 1996). The number of candidate tuning sites was then extended to include additional sites identified from the crystal structure of bovine rhodopsin (Palczewski et al., 2000) that are again either adjacent to the retinal binding pocket or the Schiff base. These include part of extracellular loop 2 between helices 4 and 5 that folds deeply into the centre of the molecule, with residues 186190 contributing to the chromophore-binding pocket. Finally, site 181 was also included, since a Glu181Gln substitution has been shown to result in a 10 nm LW-shift in bovine rhodopsin (Terakita et al., 2000). The Glu at this site is, however, totally conserved across all the deep-sea fish species.
The identity of these residues and their relative position in the helices/loop regions is shown in Table 2. The following analysis of the amino acid sequences of deep-sea fish rod opsins focuses on changes at these sites.
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In contrast to M. niger, A. tittmani probably possesses two opsin genes. Phylogenetic analysis indicates that the sequence we have obtained is the rod opsin orthologue (see Fig. 1), and a comparison of the deduced amino acid sequence with that of M. niger suggests that it would generate a pigment with max of not more than 520 nm. The key features are the presence of Tyr261 and Ile292 in both species and the absence of any other modifications such as a chloride-ion binding site (Wang et al., 1993) that would indicate a LW shift to 581 nm. It also lacks Phe208Tyr, identified above as a potential candidate for the LW shift of the M. niger pigment, indicating that substitutions at 261 and 292 may together be sufficient to shift the
max of the pigment in both species to approx. 520 nm, with the substitution at 208 responsible for the 5 nm SW shift of the M. niger pigment compared with the A. tittmani pigment.
Eight of the stomiiforms, including P. guernei, fall into a group with max values ranging from 489 nm to 481 nm. Potential tuning substitutions amongst this group are present at sites 51, 160, 186 and 294, but there is no consistent pattern of substitution that would account for the spectral shifts; it is unlikely therefore that any of these substitutions have an effect on spectral tuning. In contrast, species with
max values <480 nm all possess Glu122Gln. This substitution in bovine and human rod opsins is known to result in a 1520 nm shortwave shift (Sakmar et al., 1989; Nakayama and Khorana, 1990; Imai et al., 1997), sufficient therefore to account for the shift from 489 nm to 477 nm present in the two Argyropelecus sp. and in Vinciguerria nimbaria. The latter species differs from the former two at two other sites, 51 and 264, although the identical
max values of these three species would seem to rule out a role for the substitutions at these sites.
How do these amino acid substitutions fit in with the classification of the Stomiiformes? For the loosejaws A. tittmani, M. niger and P. guernei, the most parsimonious sequence of events is that the Phe261Tyr and Ser292Ile substitutions in the LW-shifted pigments of A. tittmani and M. niger occurred after the separation of the lineage leading to P. guernei, and this is supported by the phylogenetic analysis shown in Fig. 1. The acquisition of the Glu122Gln substitution is a little more complicated. It is present in A. aculeatus and A. gigas from the family Sternoptychidae, but in only one of the two members of the family Photoichthyidae (V. nimbaria). All three species have a max at 477 nm. This tuning substitution therefore either occurred separately in the two families, or was lost in the other member of the Photoichthyidae. Phylogenetic analysis of the pattern of substitutions supports the former explanation (Fig. 1).
Species from the Order Myctophiformes
The five myctophid species examined have similar max values to the mid-range stomiiforms, that is the group with
max values of 489481 nm. They differ from this group, however, at three potential tuning sites, possessing Gln rather than Glu at 122, Ser rather than Ala at 132, and Ala rather than Ser at 292. The substitutions at sites 122 and 292 would be expected to have opposite tuning effects and would account therefore for the positioning of the
max values of these species in the mid-range. The effect if any of Ser132 is difficult to gauge. Lampanyctus alatus differs from the other four myctophid species at site 83; this substitution may account therefore for the small SW shift of L. alatus compared to the other myctophid species. There are also differences at site 51 and 264 but, as found for the stomiiforms, these substitutions are found in species with very similar or identical
max values and are unlikely to be involved in tuning.
Species from the Order Gadiformes
The gadiforms are represented by two species, Phycis blennoides and Coryphaenoides guntheri. Unfortunately, it proved impossible to extend the 3' end of the rod opsin sequences in these species up to the end of the transmembrane region of helix 7. The sequence of this region therefore falls short of two candidate sites, 306 and 307 (Fig. 2). These sites are completely invariant, however, in all other species and unlikely therefore to be involved in tuning.
The max values for C. guntheri is SW-shifted by 15 nm compared to that of P. blennoides. These rod opsin gene sequences differ at three candidate tuning sites, 122 where C. guntheri uniquely possesses Val, 207 where Met in C. guntheri is replaced by Ile in P. blennoides, and 292 where Ser in C. guntheri is replaced by Ala in P. blennoides. This latter substitution is capable of generating a 15 nm shift by itself and may account therefore for the spectral location of the P. blennoides pigment at 494 nm. In support of this, a Glu122Ile substitution generated in chicken rod opsin was without effect on spectral tuning (Imai et al., 1997), so it is probable that the similar Glu122Val substitution is also without effect. The effect of the Met207Ile substitution remains uncertain. Finally, the rod opsins of gadiforms differ from those of the stomiiforms in possessing Asp rather than Asn at site 83. In this regard, they are similar to the majority of the myctophids.
Species from the Order Ophidiiformes
The two ophidiiforms are Bassozetus compresis and Cataetyx laticeps; the rod opsin gene sequence for the latter species was originally reported by Hope et al. (Hope et al., 1997). Both species have SW-shifted max values at 476 nm and 468 nm, respectively. In fact, C. laticeps has the shortest
max of any species examined in this study. Both species possess Ser292, which would account for much of the SW shift, and they also possess Ser124. The additional SW shift of
nm in C. laticeps pigment may be accounted for by the Thr300Ile substitution.
Species from the Order Osmeriformes
The max values of the rod pigment in the two osmeriform species, Conocara salmonea and Alepocephalus bairdii, are 480 nm and 476 nm respectively. Both possess Ser292, which would account for the SW shift of both pigments. They differ at two potential tuning sites, 132 and 168. Site 168 is, however, without effect when substituted in bovine rod opsin (Phyllis Robinson, personal communication); the 4 nm SW shift in the rod opsin of A. bairdii is probably therefore the result of the replacement of polar Ser132 by Val.
Species from the Order Beryciformes
The rod visual pigments in two species of beryciforms, Anoplogaster cornuta and Hoplostethus mediteranus, have max values of 485 nm and 479 nm, respectively. The latter species was first studied by Hope et al. (Hope et al., 1997). Ser292 is again present in both species but they differ at site 299, where polar Ser rather than Ala is present in the opsin of A. cornuta with the slightly longer
max.
Species from the Order Aulopiformes
The aulopiforms are represented by two species from the same genus, Bathysaurus ferox and B. mollis, with similar max values of 481 nm and 479 nm, respectively. Both species again possess Ser124 and Ser292, plus Thr rather than Ala at 299.
Evolution of rod opsins
Overall, therefore, substitutions at only nine sites effectively account for the spectral differences seen in the different species, although the involvement of other sites with small effects cannot be discounted and the mechanism of tuning between 489 nm and 480 nm in the stomiiforms remains to be established. These nine sites are shown in Fig. 3, where they have been placed on to a phylogeny based on a classical cladistic analysis (Nelson, 1995). Of the Superorders examined in this study, the most basal is the Protoacanthopterygii, followed by the Stenopterygii, Cyclosquamata, Scopelomorpha, Paracanthopterygii and Acanthopterygii. The species have been grouped according to family or sub-family divisions as appropriate (Table 1). Where more than two members of a sub-family are present, they have been grouped according to the neighbour-joining (Saitou and Nei, 1987) analysis of the nucleotide sequence of the rod opsin gene presented in Fig. 1.
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Discussion |
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Candidate spectral tuning sites have been identified by mapping the amino acid sequence of each fish rod opsin onto a three-dimensional model (Baldwin, 1993; Schertler and Hargrave, 1995; Baldwin et al., 1997). Potential tuning sites are identified as those that either point into the chromophore-binding pocket or face other helices. This set of sites was then extended to include all sites shown by Palczewski et al. (Palczewski et al., 2000) to be in close proximity to the chromophore or Schiff base. The stomiiforms form the largest group studied, comprising 13 species with max values for their rod opsins that range from 522 nm to 477 nm. Two substitutions, Phe261Tyr and Ser292Ile, appear largely responsible for the shift from the more typical
max values of between 489 nm and 483 nm to the longer wavelengths of A. tittmani and M. niger at around 520 nm. The additional shift to below 480 nm can be accounted for by the replacement of charged Glu by uncharged Gln at site 122. What remains unclear from this data set is the mechanism of the smaller shifts in the 489481 nm region.
When the rod opsin sequences of the fish from the other six Orders of Euteleostei are included alongside the stomiiforms, a further six candidate tuning sites can be identified, making a total of nine sites (Fig. 3). Of these however, only four at residues 83, 122, 124 and 292 are commonly substituted in the different species. Asp83Asn was originally proposed by Hope et al. (Hope et al., 1997) to be one of the main substitutions for the tuning of the rod opsins of deep-sea fish to max values of <490 nm, and site 292, which is commonly occupied by polar Ser or Thr, is responsible in most species for a substantial part of the SW shift. The major exception is in the myctophids, where Ala292 is present; in this group, the SW shift is achieved instead by polar Gln122. The SW-shifted pigments of stomiiforms and osmeriforms, with
max values of 480 nm and below, possess both Gln122 and Ser292. In contrast, similar SW shifts seen in the aulopiforms are achieved by Ser124, rather than Gln122, paired with Ser292.
Dartnall and Lythgoe (Dartnall and Lythgoe, 1965) and Bridges (Bridges, 1965) were the first to note that the max values of vertebrate visual pigments cluster around certain points in the spectrum. This is particularly apparent in primate M and L cone pigments, with each spectral location attributable to a particular combination of amino acid residues at three main sites (Neitz et al., 1991; Williams et al., 1992; Hunt et al., 1998; Dulai et al., 1999). The same phenomenon is seen in the rod pigments of deep-sea fish (Partridge et al., 1989; Partridge et al., 1992) and a similar explanation has been advanced, namely that the spectral location of pigments belonging to the same cluster group arises from a common set of amino acid substitutions (Douglas et al., 1998b). The pattern of substitutions across the 28 species included in this study indicates, however, that for the four most commonly used sites, this is only true for species from the same Order. In general, therefore, the presence of cluster points in deep-sea fish rod opsins cannot be attributed to the selection of a particular set of amino acid substitutions. Rather, it is achieved by different combinations of amino acids at these sites that produce the same net spectral shift. This is summarised in Table 3, where representative species from different Orders with the same or very similar
max values are listed. For each cluster group, there are at least two different combinations of residues at the four sites.
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The max of a visual pigment depends on at least two factors. Firstly, the strength of the electrostatic interaction between the Glu113 counterion and the protonated Schiff base is critical; substitutions that increase the strength of this interaction and thereby stabilise the ground state will result in a SW shift, whereas those that reduce it will produce a LW shift (Blatz et al., 1971; Kakitani et al., 1985). Secondly, photoexcitation of the chromophore induces a significant increase in
electron delocalization and a corresponding change in dipole moment, with a shift of net positive charge towards the ß-ionone ring upon excitation (Kropf and Hubbard, 1958; Mathies and Stryer, 1976). Interactions with charged, polar or polarizable residues that alter delocalization will lead to a change in the energy difference between ground and excited states. An increase in delocalization will result in a LW shift in the absorbance spectrum, and a decrease in a SW shift. Sites 83, 292, 299 and 300 cluster around the protonated Schiff base and negative counterion. Asp83 in bovine rhodopsin, although not directly involved in interactions with the Schiff base, is important in constraining the position of helices 2, 3 and 4 via links with other residues (Palczewski et al., 2000). The Asp83Asn substitution in deep sea fish involves a charge change and may have a consequential effect, therefore, on helix positioning in relation to the chromophore, thereby resulting in a stabilisation of the Schiff base counterion. Ala299 is also involved in an inter-helical constraint to the kinked region of helix 6 that may be disrupted on photoactivation (Palczewski et al., 2000). The other two substitutions (Ser292Ala, Ile/Leu300Thr) will both result in a change in the polar environment of the Schiff base. Sites 122, 124, 132 and 261 are all close to the polyene chain of retinal. The residue at site 122 is involved in the interaction between helix 3 and the ß-ionone ring of retinal and is one of only three sites that form the cytoplasmic aspect of the retinal binding pocket (Palczewski et al., 2000). The other two are 261 and 265. Substitutions at site 261 are known to cause spectral shifts in primate red and green pigments (Merbs and Nathans, 1993; Asenjo et al., 1994) and are implicated in this study, whereas Trp265 is totally conserved across all deep-sea fish species. Finally, site 208 is in helix 5, in a position to interact via a change in polar group with the ß-ionone ring of the chromophore.
The residue at site 122 is known to be important in determining the rate metarhodopsin II decay; in site-directed mutagenesis of chicken rod opsin, Glu122Gln or Glu122Ile produced pigments that decay at a significantly faster rate than wild type (Imai et al., 1997). If this feature extends to deep-sea fish, in addition to its effect on spectral tuning, the Glu122Gln substitution present in the three most SW-shifted stomiiform species, in all five species of mytophids, and in two species of osmeriforms, will also result in a more rapid decay of metarhodopsin II and thereby reduce the amplification of the phototransduction cascade. The Glu122Val substitution present in gadiforms, although lacking an effect on max, may also have this effect on phototransduction.
A number of the sites identified in this study have been shown to be involved in the tuning of visual pigments in other species. In particular, substitutions at site 261 play a major role in the tuning of primate MW and LW pigments (Neitz et al., 1991; Williams et al., 1992; Merbs and Nathans, 1992; Asenjo et al., 1994), and substitutions at sites 83 and 292 have been implicated in the SW shifts of rod pigments in different species of cottoid fish in Lake Baikal (Hunt et al., 1996), in bovine (Sun et al., 1997) and in the dolphin (Fasick et al., 1998). Such substitutions are clearly separate events and are examples therefore of convergent evolution, a common feature of opsin gene evolution (Hunt et al., 1998), which must reflect the relatively limited number of sites that can change to give the required spectral shift and still result in a fully functional pigment. Convergent evolution must also be the explanation for the occurrence of repeat substitutions at certain of the key sites identified in the deep-sea rod opsins examined in this study.
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Acknowledgments |
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