Avian ultraviolet vision and frequency-dependent seed preferences
Ecology of Vision Research Group, School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK
*Author for correspondence (e-mail: s.c.church{at}bristol.ac.uk)
Accepted April 19, 2001
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Summary |
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Key words: zebra finch, Taeniopygia guttata, ultraviolet, colour vision, seed preference, foraging, frequency-dependent selection.
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Introduction |
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Avian colour vision
Human colour perception is derived from differential stimulation of three types of single cone cell in the retina. These cones have peak sensitivities (max) at wavelengths of approximately 560nm (red), 530nm (green) and 420nm (blue) (Dartnall et al., 1983), and human spectral sensitivity ranges from approximately 400 to 700nm. In contrast, physiological and behavioural studies suggest that diurnal birds almost always possess four spectrally distinct single-cone types (reviewed by Cuthill et al., 2000), with spectral sensitivity extending into the near-ultraviolet (320400nm). Microspectrophotometry of the avian retina (e.g. Bowmaker et al., 1997; Hart et al., 1998; Hart et al., 1999; Hart et al., 2000) has shown the presence of long-wave-sensitive (LWS;
max 543571nm), medium-wave-sensitive (MWS;
max 497509nm), short-wave-sensitive (SWS;
max 430463nm), and either violet-sensitive (VS:
max 402426nm) or ultraviolet-sensitive (UVS;
max 355376nm) cone types. Birds are therefore sensitive to ultraviolet wavelengths that humans cannot detect, and are also likely to possess four-dimensional or tetrachromatic colour vision (Thompson et al., 1992). While the mere possession of four cone types does not guarantee that colour vision is tetrachromatic (Neumeyer, 1992), all four cones have been shown to be involved in opponent channels in birds (Osorio et al., 1999a; Osorio et al., 1999b). Each type of avian single cone cell is also associated with a characteristic pigmented oil droplet (not found in humans), through which light is filtered prior to reaching the photopigment-containing cone outer segments (Partridge, 1989; Bowmaker, 1991). These droplets act as short-wavelength cut-off filters and narrow the range of sensitivity of each cone type, possibly increasing the discriminability of certain classes of spectra (Govardovskii, 1983; Goldsmith, 1990; Vorobyev et al., 1998) and enhancing colour constancy (Osorio et al., 1997; Vorobyev et al., 1998).
Avian ultraviolet vision and foraging behaviour
The role that ultraviolet wavelengths play in the foraging behaviour of birds is only just beginning to be understood. As an integral part of the avian visual system, it is reasonable to suppose that ultraviolet wavelengths provide some visual information which birds are able to utilize for prey detection and assessment. This assertion is strengthened when one considers that many of the invertebrates, fruits, seeds and flowers on which birds feed reflect in the ultraviolet to some extent (Silberglied, 1979; Burkhardt, 1982; Willson and Whelan, 1989; Church et al., 1998a). However, the importance of ultraviolet wavelengths in a foraging task will depend upon the ultraviolet reflectance characteristics not only of the prey but also of the background against which the prey item is viewed (Church et al., 2001). While it is true that many natural backgrounds of insects (e.g. soil, bark, leaves) do not usually reflect strongly in the ultraviolet (Kevan et al., 1996; Church et al., 1998a), other natural backgrounds, such as the sky (Silberglied, 1979), clouds (Mazokhin-Porshnyakov, 1957) and some fruits (Burkhardt, 1982; Willson and Whelan, 1989), flowers (Silberglied, 1979; Chittka et al., 1994; Dyer, 1996) and sands (Pope and Hinton, 1977) may have a strong ultraviolet component.
The handful of studies which have been carried out have provided evidence that ultraviolet wavelengths can be used by birds when foraging for seeds, berries, insects and mammals. There is also evidence that ultraviolet wavelengths enhance the conspicuousness of planktonic prey to foraging fish (Browman et al., 1994). One of the earliest demonstrations that ultraviolet wavelengths could be used by birds in a basic seed detection task was performed by Emmerton and Remy (Emmerton and Remy, 1983). They demonstrated that pigeons (Columbia livia) could forage successfully (although not very efficiently) for corn seeds presented on a diffusing plate illuminated by ultraviolet light, even though their peak foraging rate for the task was at much longer wavelengths (approximately 580nm). However, the first experiments to demonstrate the importance of ultraviolet cues in a natural setting were carried out by Viitala et al. (Viitala et al., 1995), who demonstrated that kestrels (Falco tinnunculus) used the ultraviolet-reflecting scent marks of voles to locate areas of high vole abundance; the kestrels spent more time hunting in areas containing artificial scent marks rather than in areas containing water-treated trails or no trails at all. Similar results were obtained for rough-legged buzzards (Buteo lagopus) by Koivula and Viitala (Koivula and Viitala, 1999). In contrast, laboratory experiments on adults and juveniles of another major predator of voles, Tengmalms owl (Aegolius funereus), revealed no preference for arenas containing vole scent marks under ultraviolet illumination (Koivula et al., 1997). However, A. funereus is a nocturnal predator, and owls may lack ultraviolet cones (Bowmaker and Martin, 1978).
Ultraviolet wavelengths may also be used by birds foraging for fruits and berries. Some fruits, particularly those with waxy layers or blooms, reflect ultraviolet wavelengths, and it had long been postulated (Burkhardt, 1982) that these wax layers served to attract birds. Siitari et al. (1999) showed that adult redwings (Turdus iliacus) exhibited a significant preference for bilberries (Vaccinium myrtillus) in which the waxy, ultraviolet-reflecting layer was intact over berries with the wax rubbed off. This preference disappeared when ultraviolet cues were experimentally removed. This preference for ultraviolet-reflecting berries was probably learned, since juveniles failed to show similar preferences (Siitari et al., 1999).
Insects are important prey items for birds and many species, particularly among adult Lepidoptera, reflect ultraviolet strongly (Silberglied, 1979). However, avian ultraviolet vision may also have a role to play when searching for cryptic prey species. Church et al. (Church et al., 1998b) looked at the behaviour of blue tits (Parus caeruleus) searching for cabbage moth (Mamestra brassicae) or winter moth (Operophtera brumata) caterpillars on natural leafy backgrounds in a laboratory arena. They found that the blue tits took longer to find the first prey item in trials where ultraviolet wavelengths were removed from the illuminating light. This effect was greatest when the ultraviolet contrast between prey and background was largest, namely M. brassicae against a cabbage leaf. However, this reduction in foraging performance was temporary, presumably because the birds were subsequently able to learn to attend other salient visual cues (Church et al., 1998b). Since these experiments were carried out on cryptic prey, they are likely to represent conservative tests of the effects of ultraviolet wavelengths on foraging because most natural visual backgrounds (e.g. leaves, bark, soil) reflect relatively little ultraviolet light.
Evolutionary and ecological implications of avian ultraviolet sensitivity
While the use of ultraviolet wavelengths by foraging birds is now becoming well established, the evolutionary and ecological consequences of this fact have barely been explored. One major consequence is that our understanding of animal protective colour patterns (e.g. crypsis, warning coloration, mimicry) that have evolved in response to selection by birds is incomplete unless we consider the wavelengths to which birds are sensitive (Church et al., 1998a; Majerus et al., 2000; Church et al., 2001). Consideration of the ultraviolet information available to birds could radically change our interpretation of the function of a given colour pattern. For example, in the grey shoulder knot (Lithophane ornitopus) caterpillar, which appears moderately cryptic against a leafy background to the human eye, maximum reflectance is in the ultraviolet. As a result, it is feasible (although not yet tested) that the grey shoulder knot is aposematic rather than cryptic, and is advertising distastefulness to birds via an ultraviolet communication channel (Church et al., 1998a).
Variation in the amount of ultraviolet present in ambient light spectra might also have important ecological consequences (Endler, 1993; Endler, 1997) via its impact on avian foraging behaviour. The spectral quality of light in terrestrial ecosystems is very diverse, varying with weather, time of day and local habitat geometry (Endler, 1993). For example, short wavelengths tend to dominate at dawn and dusk, and in certain shaded areas of woodland (Endler, 1993). This spectral variation has important consequences for the evolution and diversity of animal colour patterns and for visual perception by animals living in spectrally diverse environments (Endler and Théry, 1996; Fleishman et al., 1997). Crucially, the perception of the colours of prey by birds can change with the spectral quality of the illuminating light (Endler, 1990), although the magnitude of this effect will depend on how effective neural mechanisms of colour constancy are. If light spectra are sufficiently variable, it is likely that some fundamental processes that underpin population and community dynamics (e.g. visually mediated prey preferences) will be affected. Indeed, Endler (Endler, 1997) states that "Any factor that alters the function, reception and perception of colour can have dire consequences at the population, species or subspecies level". While this general argument is not restricted to ultraviolet wavelengths, it is clear that variation in the ultraviolet component of irradiance spectra has the potential to affect the visually guided foraging behaviour of birds.
Avian colour vision and frequency-dependent seed preferences
Experimental studies which have addressed the effect of ultraviolet wavelengths on foraging behaviour have generally focussed either on whether the bird is simply able to detect ultraviolet or whether the birds foraging performance (in terms of foraging rate or simple prey preferences) is affected by the addition or subtraction of ultraviolet wavelengths. However, since foraging behaviour is inextricably linked with population dynamics (Fryxell and Lundberg, 1994), it is possible that changes in foraging behaviour due to the amount of ultraviolet present in the environment could have ecological implications. Here, we examine the effect of varying the ultraviolet component of ambient light on frequency-dependent selective predation (switching) by avian seed predators (Allen, 1988; Greenwood, 1984). Frequency-dependent selection is an important ecological process because apostatic frequency-dependent selection (the over-predation of common prey types) tends to promote stability and diversity in prey populations by virtue of favouring the survival of relatively rare prey (Clarke, 1962; Murdoch and Oaten, 1975; Hassell, 1978), while anti-apostatic selection (the overpredation of rare prey) tends to be highly destabilizing (Chesson, 1984). Thus, if the strength and/or direction of frequency-dependent seed selection depends upon the nature of the light environment, it could have important consequences for the local dynamics and diversity of plant populations.
In addition to considering the effects of ultraviolet wavelengths on frequency-dependent selection, we also examine the effect of wavebands in the human-visible spectrum on selectivity. It is arguable that previous research in this field has focussed on ultraviolet wavelengths at the expense of other wavelengths to which birds are sensitive. This could help to perpetuate the notion that ultraviolet wavelengths are somehow special compared to other wavelengths. Only by comparing the effects of different wavebands on behaviour can we identify which wavelengths are most important for a given behavioural task (see also Maddocks et al., 2001; Hunt et al., 2001).
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Materials and methods |
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Experiment 1: the effect of ultraviolet wavelengths on frequency-dependent seed preferences
In this experiment, we examined the frequency-dependent seed preferences of eight male zebra finches foraging for red and white millet seeds on a sand background in the presence or absence of ultraviolet wavelengths. In each trial, 200 red and white seeds were presented in a ratio of either 9:1 or 1:9 (i.e. 180:20 or 20:180). Foraging trials were carried out in a purpose-built indoor arena (1mx0.8mx0.6m) adapted from one used in previous studies (Church et al., 1998b). The inner surfaces of the arena were painted with a black vinyl matt emulsion, which had very low reflectance in the wavelength range 300700nm. A window (0.55mx0.75m) on the top surface of the arena was fitted with either an ultraviolet-blocking (UV-) or ultraviolet-transmitting (UV+) filter (Fig.1A). The arena was illuminated by eight 100W ultraviolet-emitting fluorescent tubes, powered by 240V, 71W, 3540kHz ballasts mounted above the filters (as used by Church et al., 1998b). A cage containing four companion male zebra finches was attached to one end of the arena so that test individuals could forage in the normal context of a flock. These companion birds were not experimental subjects.
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Seed selection was measured under four experimental treatments: (i) UV+ filter, 90% red seeds; (ii) UV+ filter, 10% red seeds; (iii) UV- filter, 90% red seeds; (iv) UV- filter, 10% red seeds. A balanced, randomised repeated-measures design was used, with all treatments presented to the subjects in a random order and all four treatments being carried out once each day. Individuals received each treatment once, with each of the four trials being carried out on alternate days of the experiment. The birds were deprived of food for 3h prior to the start of a trial. Trials were conducted between 12:00h and 17:00h each day and lasted for 1h. At the end of each trial, birds were removed from the arena and the number of each seed type remaining was counted.
Initial habituation to the foraging arena took place during the week prior to the experiment. Individuals were subject to one UV- and one UV+ habituation trial. Here, birds were given an equal number of red and white seeds (100 of each) and were again tested in a random order.
Experiment 2: the effect of different spectral regions on frequency-dependent seed preferences
The protocol of experiment 2 was virtually identical to that of experiment one, with the exception that different filters were used to manipulate ambient light. As in experiment 1, the experimental subjects were eight male zebra finches. In this experiment, though, we used filters that were broadly designed to block out the input to each of the cone cells of the zebra finch. This was achieved by using four different colour filters (Rosco SupergelTM filters number 73 peacock blue, 339 broadway pink, 14 straw yellow, and Lee filter number 299 ultraviolet; Fig.1B), with each filter blocking out a different part of the light spectrum (long wavelengths, LW-, medium wavelengths, MW-, short wavelengths, SW- and ultraviolet wavelengths, ultraviolet-, respectively). The filters were also selected so that the total quantal flux transmitted was approximately equal over the wavelength range 300700nm.
The frequency-dependent seed selection of each subject was measured under all eight combinations of filter (four levels: ultraviolet-, SW-, MW- or LW-) and frequency (two levels: 10% or 90% red seeds). Subjects were habituated to each of the four filter types for a total of 16h in their holding rooms, during the 2 weeks prior to the start of the experiment. They also underwent initial habituation to the filter in the foraging arena. As with experiment 1, this was a balanced, randomised, repeated-measures design.
Spectrophotometric measurements
We measured reflectance spectra from samples of the red and white millet seeds and their sand background. Measurements were made at 0° to normal using a Zeiss MCS 500 spectrophotometer with samples illuminated at 45° to normal by a Zeiss CLX 500 xenon light source. The numerical apertures of the receiving and illuminating optics were approximately 1.9 and 1.5, respectively. The diameter of the measuring spot was 2mm. Eight spectra were recorded for the sand and each of the seed types at 0.81nm intervals from 300 to 700nm and measured relative to a SpectralonTM 99% reflectance standard.
Data analysis
The ß index (Manly, 1974) was used as a measure of preference in each experimental trial. In our experiments, with prey not replaced during experimental trials, the preference for red millet seeds, ßred was estimated by Equation1:
| (1) |
where R and W are the numbers of red and white millet seeds present at the start of a trial and r and w are the numbers remaining at the end of a trial. ß lies in the range 01, with ßred=1 representing exclusive preference for red seeds and ßred=0 representing total rejection of red seeds. ß cannot be calculated when all of one type of prey are eaten. On the few occasions when this occurred, a ß value was calculated by setting the number of remaining prey equal to 0.0001.
Since individual birds were subjected to more than one trial in each experiment, all statistical analyses consisted of repeated-measures analysis of variance (ANOVA).
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Results |
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Experiment 1
We found no significant effect of either filter (F1,7=2.51, P=0.157) or frequency (F1,7=1.04, P=0.341) on the preferences of zebra finches for red seeds. However, there was a significant interaction between filter and frequency (F1,7=11.02, P=0.013), with weak apostatic selection (i.e. an increased preference for red seeds when common) under UV- treatments and a stronger anti-apostatic effect in the UV+ treatments (Fig.2). When analyzed independently, the anti-apostatic effect under the UV+ treatment was of borderline significance (F1,7=5.38, P=0.053), while the apostatic effect under the UV- treatment was not significant (F1,7=0.25, P=0.633). There tended to be an overall preference for white seeds over red seeds (i.e. mean ßred value for each treatment was less than 0.5), but this frequency-independent effect was not significant (t=1.65, d.f.=7, P=0.14).
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Experiment 2
In this experiment, we found no evidence that seed preferences were frequency-dependent or that these preferences were modified by different light environments. Thus, there were no significant main effects of either filter (F3,21=0.18, P=0.911) or frequency (F1,21=0.67, P=0.440) and no significant interaction between filter and frequency (F3,21=0.65, P=0.590; Fig.3). Inevitably, all individual tests for frequency-dependent selection were non-significant (UV-: F1,7=0.00, P=0. 997; SW-: F1,7=2.35, P=0.169; MW-: F1,7=1.08, P=0.333; LW-: F1,7=0.67, P=0.439). As with experiment 1, there was no overall frequency-independent preference (t=1.3, d.f.=7, P=0.24) even though the mean ßred value for each treatment was less than 0.5.
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Discussion |
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It would be unwise to suggest that ultraviolet wavelengths are somehow special in these experiments. The only filter type that produced a near-significant frequency-dependent effect was UV+ (i.e. full-spectrum illumination). Thus, rather than ultraviolet being particularly important, it might be that the mechanisms underlying frequency-dependent selection in these experiments might only be expressed if the illuminant is essentially white (i.e. all four of the single cone types of the zebra finch are highly stimulated) (Cuthill et al., 2000). The removal of different wavebands (i.e. UV-, SW-, MW- or LW-) in experiment 2 would have meant that the seeds in these treatments might have been perceived by the zebra finches as more similar in colour to each other as a result of the inevitable loss of some visual information. If, as has been suggested, anti-apostatic selection at high density arises because of a confusion effect, due to the contrast between the rare seed type against a background of common seeds (Greenwood, 1984), then it is likely that the degree of confusion would be reduced if part of the illuminating spectrum was removed, resulting in the selection of the birds becoming closer to random, as observed. However, since this was a long wavelength-dominated task, we would not necessarily expect this effect to be identical for all wavebands.
It is not clear whether the results we obtained were due to the manipulations of the light environment affecting the hue of the seeds and background, their brightness or a combination of both. In experiment 1, the UV+ and UV- filters only differed in light transmitted at ultraviolet wavelengths, resulting in a higher total quantal flux in the UV+ treatment than the UV- treatment. In experiment 2, approximate matching of the quantal flux for the coloured filters necessitated a lowering of the mean transmission of filters compared to experiment 1. Such changes in brightness are consistent with our results, with the strongest frequency-dependent effect being observed under the brightest treatment (i.e. full-spectrum illumination in experiment 1). However, in a similar study that investigated the effect of different light environments on the simple seed preferences of zebra finches, Maddocks et al. (Maddocks et al., 2001), contrary to the results presented here, found that long wavelengths had the greatest effect on relative seed consumption and that there was no difference between seed preferences under UV+ and UV- treatments. Thus, in these experiments, the finches were least selective in the brightest (UV+) treatments. Other behavioural studies that have explicitly manipulated brightness (e.g. Bennett et al., 1996), while testing for the effects of ultraviolet, have found no significant effect of brightness on behaviour. Recently, however, it has been demonstrated that achromatic cues may be more important than chromatic cues for chicks foraging for prey items around 2mm in diameter (Osorio et al., 2001), similar to the size of the seeds in our experiments. While our experiments were not designed to elucidate the visual mechanisms underlying any frequency-dependent effects, it is clear that more work is needed in this area.
It is worth noting that Manlys ß index (Manly, 1974) can be subject to apostatic biases when less than 50% of the total prey items available are consumed (Weale, 1992; Church et al., 1996; Church et al., 1997). Since the number of seeds consumed in our experiments was usually between 20% and 25%, such biases were likely to be present. One consequence of this is that the borderline anti-apostatic effect under the UV+ filter in experiment 1 is likely to be a slight underestimate of the true anti-apostatic effect. In experiment 1, we also found that significantly fewer seeds were consumed in trials in which red was common than when red was rare. This could be due to the general, albeit non-significant, trend towards an overall (frequency-independent) preference for white seeds. However, this difference would not have affected the qualitative interpretation of results with regards to the effects of ultraviolet wavelengths, since any biases would have had similar effects under each filter treatment.
These data are intriguing in that they suggest the possibility that changes in ambient light spectra could, whether mediated by changes in hue or brightness, change the strength and direction of frequency-dependent selective predation. This will have concomitant consequences for plant population dynamics on a local and global scale (Greenwood, 1985). Since the spectral composition and quantal flux of ambient light varies from habitat to habitat and even within the same habitat as weather and time of day vary (Endler, 1993), it raises the possibility that selection pressures imposed via frequency-dependent selective predation may change on a daily or seasonal basis. Thus, the evolutionary and ecological consequences of selection might change depending on such fine-grained events as the time of day of maximum predation. Despite these theoretical possibilities, it is not clear at present how important these effects are likely to be in natural ecosystems. Certainly, the experimental treatments described here represent quite extreme manipulations of ambient light spectra. Spectral variation in terrestrial systems, while clearly not insignificant, is by no means as high as that found in aquatic habitats (Chiao et al., 2000). One crucial factor that is likely to be important in determining the real-world implications of variation in ambient light spectra is how good avian colour constancy is. Colour constancy is the phenomenon whereby objects are perceived as the same colour despite changes in the illuminant (Hurlbert, 1996). It has been suggested that the narrowing of spectral sensitivity of the single cone cells of birds will increase their ability to be colour constant (Vorobyev et al., 1998). Indeed, recent models of avian tetrachromatic vision (Vorobyev and Osorio, 1998; Vorobyev et al., 1998) make the explicit assumption that birds are colour constant. However, it must also be true that avian colour constancy cannot be perfect when significant behavioural effects have resulted from modification of the ultraviolet component of ambient light (e.g. Bennett et al., 1996; Bennett et al., 1997; Hunt et al., 1997; Hunt et al., 1998; Hunt et al., 1999; Church et al., 1998b). It seems logical to conclude that there must be some point at which colour constancy breaks down. Since we currently lack experimental data regarding (i) the ability of birds to be colour constant, and (ii) the degree of natural variation in ambient light spectra that birds encounter when performing specific foraging tasks, we are unable to answer this question. In addition, what general information does exist on natural variation in terrestrial ambient light environments does not, unfortunately, extend to measurement of ultraviolet levels (Endler, 1993; Chiao et al., 2000).
In summary, our experiments do not necessarily demonstrate that ultraviolet wavelengths are in any way special in their effects on the frequency-dependent seed preferences of zebra finches. While it is important to determine the role of ultraviolet wavelengths in any behavioural task, it may prove more illuminating to consider the whole range of wavelengths to which birds are sensitive, rather than focusing explicitly on one waveband. Our data also highlight the fact that there are gaps in our knowledge concerning both the ecological consequences of visually mediated behaviours of birds (including ultraviolet wavelengths) and the visual mechanisms underlying these behaviours.
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Acknowledgments |
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References |
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Allen, J. A. (1988). Frequency-dependent selection by predators. Phil. Trans. R. Soc. Lond B 319, 485503.[Medline]
Allen, J. A. and Anderson, K. P. (1984). Selection by passerine birds is anti-apostatic at high prey density. Biol. J. Linn. Soc. 23, 237246.
Allen, J. A., Raison, H. E. and Weale, M. E. (1998). The influence of density on frequency-dependent selection by wild birds feeding on artificial prey. Proc. R. Soc. Lond. B 265, 10311035.
Andersson, S. and Amundsen, T. (1997). Ultraviolet colour vision and ornamentation in bluethroats. Proc. R. Soc. Lond. B 264, 15871591.
Andersson, S., Örnborg, J. and Andersson, M. (1998). Ultraviolet sexual dimorphism and assortative mating in blue tits. Proc. R. Soc. Lond. B 265, 445450.
Bennett, A. T. D. and Cuthill, I. C. (1994). Ultraviolet vision in birds: what is its function? Vision Res. 34, 14711478.[Medline]
Bennett, A. T. D., Cuthill, I. C. and Norris, K. J. (1994). Sexual selection and the mismeasure of color. Am. Nat. 144, 848860.
Bennett, A. T. D., Cuthill, I. C., Partridge, J. C. and Lunau, K. (1997). Ultraviolet plumage colors predict mate preferences in starlings. Proc. Natl. Acad. Sci. USA 94, 86188621.
Bennett, A. T. D., Cuthill, I. C., Partridge, J. C. and Maier, E. J. (1996). Ultraviolet vision and mate choice in zebra finches. Nature 380, 433435.
Bowmaker, J. K. and Martin, G. R. (1978). Visual pigments and colour vision in a nocturnal bird, Strix aluco (Tawny Owl). Vision Res. 18, 11251130.[Medline]
Bowmaker, J. K. (1991). Visual pigments, oil droplets and photoreceptors. In Vision and Visual Dysfunction, vol. 6 (ed. P. Gouras), pp. 108127. Boston: CRC Press Inc.
Bowmaker, J. K., Heath, L. A., Wilkie, S. E. and Hunt, D. M. (1997). Visual pigments and oil droplets from six classes of photoreceptor in the retinas of birds. Vision Res. 37, 21832194.[Medline]
Browman, H. I., Novales-Flamarique, I. and Hawryshyn, C. W. (1994). Ultraviolet photoreception contributes to prey search behaviour in two species of zooplanktivorous fishes. J. Exp. Biol. 186, 187198.
Burkhardt, D. (1982). Birds, berries and ultraviolet. Naturwissenschaften 69, 153157.[Medline]
Burkhardt, D. (1989). Ultraviolet vision: a birds eye view of feathers. J. Comp. Physiol. A 164, 787796.
Chesson, P. L. (1984). Variable predators and switching behaviour. Theor. Population Biol. 26, 126.
Chiao, C.-C., Cronin, T. W. and Osorio, D. (2000). Color signals in natural scenes: characteristics of reflectance spectra and effects of natural illuminants. J. Opt. Soc. Am. A 17, 218224.
Chittka, L., Shmida, A., Troje, N. and Menzel, R. (1994). Ultraviolet as a component of flower reflections, and the colour perception of hymenoptera. Vision Res. 34, 14891508.[Medline]
Church, S. C., Allen, J. A. and Bradshaw, J. W. S. (1996). Frequency-dependent food selection by domestic cats: a comparative study. Ethology 102, 495509.
Church, S. C., Bennett, A. T. D., Cuthill, I. C., Hunt, S., Hart, N. S. and Partridge, J. C. (1998a). Does Lepidopteran larval crypsis extend into the ultraviolet? Naturwissenschaften 85, 189192.
Church, S. C., Bennett, A. T. D., Cuthill, I. C. and Partridge, J. C. (1998b). Ultraviolet cues affect the foraging behaviour of blue tits. Proc. R. Soc. Lond. B 265, 15091514.
Church, S. C., Cuthill, I. C., Bennett, A. T. D. and Partridge, J. A. (2001). Avian ultraviolet vision and its implications for insect defensive coloration. In Insects and Birds (ed. H. van Emden). Andover: Intercept. (In press.)
Church, S. C., Jowers, M. J. and Allen, J. A. (1997). Does prey dispersion affect frequency-dependent predation by wild birds? Oecologia 111, 292296.
Clarke, B. C. (1962). Balanced polymorphism and the diversity of sympatric species. In Taxonomy and Geography (ed. D. Nichols), pp. 4770. Oxford: Systematics Association.
Cuthill, I. C. and Bennett, A. T. D. (1993). Mimicry and the eye of the beholder. Proc. R. Soc. Lond. B 253, 203204.
Cuthill, I. C., Partridge, J. C., Bennett, A. T. D., Church, S. C., Hart, N. S. and Hunt, S. (2000). Ultraviolet vision in birds. Adv. Stud. Behav. 29, 159214.
Dartnall, H. J. A., Bowmaker, J. K. and Mollon, J. D. (1983). Microspectrophotometry of human photoreceptors. In Colour Vision: Physiology and Psychophysics (ed. J. D. Mollon and L. T. Sharpe), pp. 6980. London: Academic Press.
Dyer, A. G. (1996). Reflection of near-ultraviolet radiation from flowers of Australian native plants. Austr. J. Bot. 44, 473488.
Emmerton, J. and Remy, M. (1983). The pigeons sensitivity to ultraviolet and visible light. Experientia 39, 1161 1163.
Endler, J. A. (1990). On the measurement and classification of colour in studies of animal colour patterns. Biol. J. Linn. Soc. 41, 315352.
Endler, J. A. (1993). The color of light in forests and its implications. Ecol. Monogr. 63, 127.
Endler, J. A. (1997). Light, behavior, and conservation of forest-dwelling organisms. In: Behavioral Approaches to Conservation in the Wild (ed. J. R. Clemmons and R. Buchholz), pp. 329355. Cambridge: Cambridge University Press.
Endler, J. A. and Théry, M. (1996). Interacting effects of lek placement, display behavior, ambient light, and color patterns in three neotropical forest-dwelling birds. Am. Nat. 148, 421452.
Fleishman, L. J., Bowman, M., Saunders, D., Miller, W. E., Rury, M. J. and Loew, E. R. (1997). The visual ecology of Puerto Rican anoline lizards: habitat light and spectral sensitivity J. Comp. Physiol. A 181, 446460.
Fryxell, J. M. and Lundberg, P. (1994). Diet choice and predator-prey dynamics. Evol. Ecol. 8, 407421.
Goldsmith, T. H. (1990). Optimization, constraint, and history in the evolution of eyes. Q. Rev. Biol. 65, 281322.[Medline]
Govardovskii, V. I. (1983). On the role of oil drops in colour vision. Vision Res. 23, 17391740.[Medline]
Greenwood, J. J. D. (1984). The functional basis of frequency-dependent food selection. Biol. J. Linn. Soc. 23, 177199.
Greenwood, J. J. D. (1985). Frequency-dependent selection by seed predators. Oikos 44, 195210.
Hart, N. S., Partridge, J. C. and Cuthill, I. C. (1998). Visual pigments, oil droplets and cone photoreceptor distribution in the European starling (Sturnus vulgaris). J. Exp. Biol. 201, 14331446.
Hart, N. S., Partridge, J. C. and Cuthill, I. C. (1999). Visual pigments, cone oil droplets, ocular media and predicted spectral sensitivity in the domestic turkey (Meleagris gallopavo). Vision Res. 39, 33213328.[Medline]
Hart, N. S., Partridge, J. C., Cuthill, I. C. and Bennett, A. T. D. (2000). Visual pigments, oil droplets, ocular media and cone photoreceptor distribution in two species of passerine: the blue tit (Parus caeruleus L.) and the blackbird (Turdus merula L.). J. Comp. Physiol. A 186, 375387.[Medline]
Hassell, M. P. (1978). The Dynamics of Arthropod Predator-Prey Systems (Monographs in Population Biology 13). Princeton: Princeton University Press.
Hunt, S., Bennett, A. T. D., Cuthill, I. C. and Griffiths, R. (1998). Blue tits are ultraviolet tits. Proc. R. Soc. Lond. B 265, 451455.
Hunt, S., Cuthill, I. C., Bennett, A. T. D. and Partridge, J. C. (1999). Preference for ultraviolet partners in the blue tit. Anim. Behav. 58, 809815.[Medline]
Hunt, S., Cuthill, I. C., Bennett, A. T. D., Church, S. C. and Partridge, J. C. (2001). Is the ultraviolet waveband a special communication channel in avian mate choice? J. Exp. Biol. 24992507.
Hunt, S., Cuthill, I. C., Swaddle, J. P., and Bennett, A. T. B. (1997). Ultraviolet vision and band colour preferences in female zebra finches, Taeniopygia guttata. Anim. Behav. 54, 13831392.[Medline]
Hurlbert, A. C. (1996). Colour vision: putting it in context. Curr. Biol. 6, 13811384.[Medline]
Jacobs, G. H. (1992). Ultraviolet vision in vertebrates. Am. Zool. 32, 544554.
Kevan, P., Giurfa, M. and Chittka, L. (1996). Why are there so many and so few white flowers? Trends Physiol. Sci. 1, 280284.
Koivula, M., Korpimaki, E. and Viitala, J. (1997). Do Tengmalms owls see vole scent marks visible in ultraviolet light? Anim. Behav. 54, 873877.[Medline]
Koivula, M. and Viitala, J. (1999). Rough-legged Buzzards use vole scent marks to assess hunting areas. J. Avian Biol. 30, 329332.
Maddocks, S. A., Church, S. C. and Cuthill, I. C. (2001). The effects of the light environment on prey choice by zebra finches. J. Exp. Biol. 25092515.
Majerus, M. E. N., Brunton, C. F. A. and Stalker, J. (2000). A birds eye view of the peppered moth. J. Evol. Biol. 13, 155159.
Manly, B. F. J. (1974). A model for certain types of selection experiments. Biometrica 30, 281294.
Mazokhin-Porshnyakov, G. A. (1957). Reflecting properties of butterfly wings and the role of ultra-violet rays in the vision of insects. Biophysics, 2, 285296.
Murdoch, W. W. and Oaten, A. S. (1975). Predation and population stability. Adv. Ecol. Res. 9, 1131.
Neumeyer, C. (1992). Tetrachromatic color vision in goldfish: evidence from color mixture experiments. J. Comp. Physiol. A 171, 639649.
Osorio, D., Jones, C. D. and Vorobyev, M. (1999a). Accurate memory for colour but not pattern contrast in chicks. Curr. Biol. 9, 199202.[Medline]
Osorio, D., Marshall, N. J. and Cronin, T. W. (1997). Stomatopod photoreceptor tuning as an adaptation to colour constancy underwater. Vision Res. 37, 32993309.[Medline]
Osorio, D., Miklósi, A. and Gonda, Zs. (2001) Visual ecology and perception of coloration patterns by domestic chicks. Evol. Ecol. 673689.
Osorio, D., Vorobyev, M. and Jones, C. D. (1999b). Colour vision of domestic chicks. J. Exp. Biol. 202, 29512959.
Partridge, J. C. (1989). The visual ecology of avian cone oil droplets. J. Comp. Physiol. A 165, 415426.
Pope, R. D. and Hinton, H. E. (1977). A preliminary survey of ultraviolet reflectance in beetles. Biol. J. Linn. Soc. 9, 331348.
Siitari, H., Honkavaara, J. and Viitala, J. (1999). Ultraviolet reflection of berries attracts foraging birds. A laboratory study with redwings (Turdus iliacus) and bilberries (Vaccinium myrtillus). Proc. R. Soc. Lond. B 266, 21252129.
Silberglied, R. E. (1979). Communication in the ultraviolet. Annu. Rev. Ecol. Syst. 10, 373398.
Thompson, E., Palacios, A. and Varela, F. J. (1992). Ways of coloring: comparative color vision as a case study for cognitive science. Behav. Brain Sci. 15, 174.
Tovée, M. J. (1995). Ultraviolet photoreceptors in the animal kingdom: their distribution and function. Trends Evol. Ecol. 10, 455460.
Viitala, J., Korpimaki, E., Palokangas, P. and Koivula, M. (1995). Attraction of kestrels to vole scent marks visible in ultraviolet light. Nature 373, 425427.
Vorobyev, M. and Osorio, D. (1998). Receptor noise as a determinant of colour thresholds. Proc. R. Soc. Lond. B 265, 351358.[Medline]
Vorobyev, M., Osorio, D., Bennett, A. T. D., Marshall, N. J. and Cuthill, I. C. (1998). Tetrachromacy, oil droplets and bird plumage colours. J. Comp. Physiol. A 183, 621633.[Medline]
Weale, M. E. (1992). Degrees of visible polymorphism and new methods for measuring frequency-dependent selective predation by predators. PhD thesis, University of Southampton, UK.
Willson, M. F. and Whelan, C. J. (1989). Ultraviolet reflectance of fruits of vertebrate-dispersed plants. Oikos 55, 341348.