1 Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
2 Section of Cell and Developmental Biology, Division of Biological Sciences, Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0380, USA
3 Gen*NY*sis Center for Excellence in Cancer Genomics and Department of Epidemiology and Biostatistics, State University of New York at Albany, One University Place, Rensselaer, NY 12144, USA
Author for correspondence (e-mail: nm3h{at}virginia.edu)
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Summary |
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Key words: Zizimin1, Dock4, Dock3, Zizimin2, Dock2, GTPase
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Introduction |
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Rho proteins are active when bound to GTP and inactive when bound to GDP. Conversion of the GDP-bound proteins to the active state is catalyzed by guanine nucleotide exchange factors (GEFs). The classical GEFs for Rho GTPases share a common motif, the Dbl-homology (DH) domain, which mediates nucleotide exchange (Cerione and Zheng, 1996). In mammals, 69 DH-domain-containing proteins have been identified, illustrating the need for selective activation of Rho proteins by different signaling pathways under diverse conditions (Rossman et al., 2005
; Schmidt and Hall, 2002
). Until recently, DH-domain-containing proteins were considered to be the universal Rho-GEFs in eukaryotes. We review here a non-conventional Rho-GEF protein family whose members lack the DH domain and instead possess a novel form of GEF domain.
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Identification of a new family of GEFs for Rho proteins |
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On the basis of domain structure, sequence similarity and phylogenetic analysis, the CZH family of proteins can be divided into four subfamilies. Almost all CZH proteins can be broadly categorized as either zizimin-related or Dock180-related proteins, because most of their sequences outside the CZH1 and CZH2 domains have similarity to one of these two proteins (Fig. 1). The zizimin- and Dock180-related proteins can each be further divided, resulting in four subfamilies: the zizimin, zir (for `zizimin-related'), Dock180 and Dock4 proteins (Figs 1 and 2, Table 1). Proteins within these subfamilies exhibit 50-65% identity in amino acid sequence.
Proteins from the zizimin subfamily have a pleckstrin-homology (PH) domain close to the N-terminus. PH domains have been implicated in targeting of proteins to membranes and in protein-protein interactions (Lemmon and Ferguson, 2000). Preliminary results indicate that the PH domain also targets zizimin 1 to membranes (N.M., M. R. Westbrook and M. A. Schwartz, unpublished). Zizimin proteins exist predominantly as dimers and the Dock180 proteins are likely to dimerize as well (Meller et al., 2004
; Yin et al., 2004
). Although the zir proteins lack a PH domain, they show
30% identity to zizimin proteins along most of their sequence.
Dock180 and Dock4 proteins have an N-terminal Src homology 3 (SH3) domain and divergent C-termini containing proline-rich motifs that bind SH3 domains to mediate intra- or inter-molecular protein interactions (Mayer, 2001). The Dock180/Dock4 subfamilies are more closely related to each other compared with the zizimin/zir proteins, sharing a similar domain structure and 40% sequence identity.
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The CZH2 domain: a novel GEF domain |
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The selectivities of different CZH proteins for Rho-family GTPases, in cells and in vitro, have been tested in binding and GTPase-activation assays. The substrates for the Dock180 and Dock4 subfamilies are Rac proteins; zizimin 1 and zizimin 2 interact selectively with Cdc42; and the substrates for zizimin 3 and zir proteins remain to be identified (Table 1).
Structure of the CZH2 domain
Although the exact boundaries of the CZH2 domain remain to be determined, it is clearly very large, containing 450-550 residues (DH domains contain only 180 residues). The sequence conservation shared by CZH2 domains is low: there is 16-17% identity in amino acid sequence between the zizimin1 and Dock180/Dock4 proteins (see supplementary material Fig. S1). This is comparable with DH domains, which share 19-29% sequence identity. Despite this, the three-dimensional structures of different DH domains are very similar (Schmidt and Hall, 2002
) and this could also be the case for CZH2 domains. The Salmonella typhimurium SopE protein is another example of a GEF for Rho proteins that bears no DH domain (Buchwald et al., 2002
; Hardt et al., 1998
; Rudolph et al., 1999
; Schlumberger et al., 2003
). Note that there is no sequence homology between CZH2 domains and the SopE GEF domain.
Zizimin1 dimerizes through its CZH2 domain. Each dimer has two individual Cdc42-binding sites, and kinetic measurements have demonstrated increased binding affinity for Cdc42 at higher Cdc42 concentrations (Meller et al., 2004). This suggests positive cooperativity in which binding of Cdc42 to one site increases the affinity of the second site. It may represent a mechanism for regulation of GEF activity by the local GTPase concentration.
Multiple alignment of CZH2 domain sequences (see supplementary material Fig. S1) reveals stretches of similarity between the Dock180- and zizimin-related proteins along most of the domain, and the predicted secondary structures are mostly similar. Yet, there is also large variability, and the conserved areas are separated by non-conserved stretches that vary substantially in length between the two groups. Therefore, although the general functions of the CZH2 domains in the Dock180- and zizimin-related proteins are similar, some aspects of the mechanism may differ significantly.
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ELMO: a Dock180 cofactor |
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Three of the five mammalian Dock180-related proteins tested so far interact with ELMO. This suggests that regulation by ELMO is a general feature of the Dock180-related proteins (Grimsley et al., 2004; Janardhan et al., 2004
; Sanui et al., 2003b
). Zizimin-related proteins have no similarity to the N-terminal part of Dock180, which mediates ELMO binding, and therefore may not interact with ELMO.
Mammals have three ELMO proteins (ELMO1, ELMO2, ELMO3), of which two have so far been shown to interact with Dock180 proteins (Grimsley et al., 2004; Gumienny et al., 2001
; Katoh and Negishi, 2003
; Sanui et al., 2003b
). ELMO regulates Dock180 function by several means. By binding to active RhoG, which resides at the cell membrane, ELMO can target Dock180 to the cell membrane, leading to Rac activation (Debakker et al., 2004
; Katoh and Negishi, 2003
). Alternatively, the ELMO PH domain can bind to and stabilize the nf-Rac-CZH2 complex (Lu et al., 2004
). Another mechanism involves the ELMO proline-rich motif. This relieves a steric inhibition within Dock180, in which the SH3 domain interacts with the CZH2 domain to block binding of Rac (Lu et al., 2005
). Binding of ELMO to the SH3 domain disrupts the interaction, allowing Rac access. Experiments with Dock2 and Dock4 indicate that this mechanism might be common to the SH3-bearing Dock180-related proteins (Lu et al., 2005
).
Dock180 and ELMO have been proposed to function as a bipartite GEF, and indeed the ELMO PH domain stabilizes the nf-Rac-Dock180-CZH2 complex (Brugnera et al., 2002; Lu et al., 2004
). However, it is not clear whether, within the ternary complex, ELMO contacts Rac directly or acts by stabilizing a Dock180 conformation favorable for Rac binding. Dock180 mutants that do not bind ELMO possess GEF activity (Cote and Vuori, 2002
; Lu et al., 2005
), which suggests that ELMO in fact merely acts as a Dock180 cofactor and the mechanism for nucleotide exchange is in Dock180 per se.
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Signal transduction by CZH proteins |
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Dock180 proteins in Drosophila also regulate migration processes. The myoblast city (Mbc) mutants display defects in border cell migration during oogenesis and migration of epidermal cells that result in incomplete dorsal closure during embryogenesis and incomplete thorax closure during metamorphosis (Duchek et al., 2001; Erickson et al., 1997
; Ishimaru et al., 2004
; Nolan et al., 1998
). The migration of border cells is guided by the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) receptor ligand PVF in an Mbc-dependent but PI3K-independent manner (Duchek et al., 2001
). The PDGF/VEGF receptor also regulates thorax closure, and Crk, ELMO, Mbc, Rac and JNK are all implicated in this pathway (Ishimaru et al., 2004
).
Engulfment
Integrins also mediate engulfment of apoptotic cells or bacteria, and Dock180 is likely to play a role in this process (Fig. 3). Engulfment of apoptotic cells driven by vß5 integrins depends on FAK, which associates with the cytoplasmic tail of ß5 and induces Rac activation through Dock180-Crk-p130CAS (Akakura et al., 2004
; Albert et al., 2000
; Wu et al., 2005
). An additional receptor for apoptotic cells, the tyrosine kinase Mer, is involved in the process probably through activation of a Src-family kinase, which activates FAK (Wu et al., 2005
). Engulfment of bacteria by mammalian cells expressing ß1 integrins, which bind to the bacterial protein invasin, is likely to involve Dock180 as well (Gustavsson et al., 2004
; Wong and Isberg, 2005
).
Genetic studies have demonstrated that regulation of cell engulfment (and migration) processes by Dock180, Crk and ELMO proteins is conserved in C. elegans (reviewed by Blelloch et al., 1999; Grimsley and Ravichandran, 2003
; Lehmann, 2001
; Reddien and Horvitz, 2004
).
The RhoG connection
Dock180 can also mediate Rac activation downstream of the small GTPase RhoG (see above). This pathway may mediate integrin-induced Rac activation, cell migration, phagocytosis and nerve growth factor (NGF)-induced neurite outgrowth (Debakker et al., 2004; Grimsley et al., 2004
; Katoh and Negishi, 2003
). The RhoG and Crk pathways might be connected, since Crk, Dock180 and ELMO can form a ternary complex (Gumienny et al., 2001
; Wu et al., 2001
), and CRK might regulate interactions between Dock180, ELMO, Rac and RhoG (Akakura et al., 2005
).
Dock2
Dock2 is expressed selectively in hematopoietic cells including lymphocytes, dendritic cells and possibly others. By contrast, Dock180 is absent from lymphocytes (Fukui et al., 2001; Hasegawa et al., 1996
, Akakura, 2004; Nishihara et al., 1999
). Studies in knockout mice have demonstrated important roles for Dock2 in lymphocyte development, homing, activation, adhesion, polarization and migration processes (Fukui et al., 2001
; Nombela-Arrieta et al., 2004
; Sanui et al., 2003a
) (reviewed by Reif and Cyster, 2002
). Translocation of the T-cell receptor (TCR) and lipid rafts to the immunological synapse is impaired in Dock2-deficient cells (Nishihara et al., 2002b
; Sanui et al., 2003a
). Dock2 associates with the TCR and is required for Rac activation by the receptor. Dock2 also associates with Vav, a DH-type GEF for Rac that plays a critical role in formation of the immunological synapse and Rac activation (Nishihara et al., 2002a
). Interestingly, the HIV-1 protein Nef associates with the Dock2-ELMO1 complex. This leads to Rac activation and inhibition of T-cell chemotaxis (Janardhan et al., 2004
). Migration requires localized Rac activation and cell polarization; Nef could therefore potentially disturb chemotaxis by inducing global Rac activation.
Dock3 and Dock4
Dock3 (also known as MOCA) is expressed predominantly in neurons and resides in growth cones and membrane ruffles (Chen et al., 2005; De Silva et al., 2003
; Namekata et al., 2004
). Dock3 overexpression increases morphological complexity and the number of neurites in differentiating PC12 cells, whereas downregulation of the protein has an inhibitory effect (Chen et al., 2005
). This suggests that Dock3 promotes neurite outgrowth. Dock3 expression also increases the level of N-cadherin, leading to cell-cell adhesion (Chen et al., 2005
). Interestingly, mutations in Dock3 may be associated with attention-deficit hyperactivity disorder (ADHD) in humans (De Silva et al., 2003
).
The gene encoding Dock4 was cloned in a screen for genes deleted during tumor progression, and in vitro and in vivo experiments have demonstrated that Dock4 possesses tumor suppressor properties (Yajnik et al., 2003). Expression of Dock4 also leads to activation of the small GTPase Rap1 and enhanced formation of adherens junctions (Yajnik et al., 2003
). Rap1 activation is required for Dock4-mediated adherens junction formation, which indicates that Rap1 may mediate Dock4 tumor suppressor activity. However, expression of Dock4 in cells also leads to activation of Rac (Lu et al., 2005
). Whether the Dock4 interaction with Rap1 and Rac is direct has not been tested. Given that Dock4 possesses a CZH2 domain (but not a Rap-GEF domain), and given the high sequence homology to Dock3 (54% identity), which acts on Rac, Rac activation by Dock4 is likely to be direct whereas Rap1 activation is probably indirect.
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CZH proteins in Saccharomyces cerevisiae, Dictyostelium and plants |
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Dictyostelium possesses at least eight CZH proteins. Several of these, like Dictyostelium Rho proteins, are noticeably divergent from one another (Rivero et al., 2001; Rivero and Somesh, 2002
) (Table 2, Figs 1 and 2). The different Dictyostelium CZH proteins might therefore correspond to distinct subgroups of Rho proteins. Dd zizA and Dd zizB appear to be involved in Dictyostelium development processes but not in chemotaxis towards cAMP (S.M. and R. Firtel, unpublished).
Green plants have Rho proteins distinct from those of other kingdoms (termed ROPs or Aracs), and these constitute a separate phylogenetic group (reviewed by Valster et al., 2000; Vernoud et al., 2003
; Yang, 2002
). Interestingly, there are no DH proteins in Arabidopsis, and the question of whether ROPs utilize GEFs, and if so which, has remained open (Valster et al., 2000
). Arabidopsis encodes a single CZH protein, SPIKE1, that was reported recently to act as ROPs-GEF (Basu et al., 2005
). Interestingly, SPIKE1 was cloned in a screen for genes controlling cytoskeletal organization, and mutations in SPIKE1 impair localized lateral microtubule clustering and polarized growth (Qiu et al., 2002
). Furthermore, yet another group of ROP-GEFs has been discovered (Berken et al., 2005
) - it is present exclusively in plants and uses a novel GEF domain that is unrelated to DH, CZH2 or the SopE GEF domains.
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Concluding remarks |
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DH-domain-containing proteins outnumber CZH proteins by 3-6-fold (Rossman et al., 2005). It is unclear why there are two classes of GEF and why the DH protein family expanded more over evolutionary time than did the CZH protein family. The information gathered so far suggests that principles of regulation of Dbl proteins, such as autoinhibition of GEF activity, formation of complexes with signaling proteins, and translocation within cellular compartments to induce activation, also apply to CZH proteins. Detailed kinetic studies with full-length proteins might reveal significant differences in the exchange mechanisms of DH and CZH proteins. Note also that DH-domain-containing proteins are much more versatile in their domain compositions (Schmidt and Hall, 2002
). This may have provided an advantage that hooked them into different signaling pathways and contributed to their expansion.
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Acknowledgments |
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Footnotes |
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* Present address: Institut des Sciences du Végétal, Centre National de la Recherche Scientifique, 1 avenue de la Terrasse, 91198 Gif sur Yvette, France
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