Cell Biology, Zoological Institute, Technical University of Braunschweig, D-38092 Braunschweig, Germany
* Present address: Medizinische Klinik I, University of Erlangen, D-91054 Erlangen
Author for correspondence (e-mail: bmj{at}tu-bs.de)
Accepted July 8, 2001
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SUMMARY |
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Key words: Diaphanous proteins, Rho-like GTPases, Profilin, Actin-dependent cell motility, Ruffling
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INTRODUCTION |
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The RBD of diaphanous proteins has been the focus of several studies. It is a target of activated Rho proteins (Imamura et al., 1997; Kohno et al., 1996; Watanabe et al., 1997). While the RBDs of mammalian diaphanous proteins bind only activated RhoA-C, one of the budding yeast diaphanous proteins also interacts with Cdc42 (Evangelista et al., 1997). The small GTPases of the Rho family, Rho, Rac and Cdc42, regulate actin filament organization (Hall, 1998; Machesky and Hall, 1996; Nobes and Hall, 1995; Ridley, 1996). In mouse fibroblasts, Rho has been shown to stimulate the formation of large, actin-based cell-matrix adhesion sites (focal contacts) and contractile microfilament bundles (stress fibers), and to induce and maintain the cleavage ring in cytokinesis (Chrzanowska-Wodnicka and Burridge, 1996; Narumiya, 1996; Ridley and Hall, 1992). Rac activity is required for membrane ruffling at the periphery of the cells and the formation of small focal complexes (Nobes and Hall, 1995; Ridley et al., 1992), and Cdc42 induces the formation of filopodia (Kozma et al., 1995; Nobes and Hall, 1995). Binding of activated Rho to the RBD of murine (m)Dia1 stimulates actin reorganization to prominent stress fibers, in cooperation with other GTPase effectors such as the Rho kinase ROCK (Nakano et al., 1999; Watanabe et al., 1999), and depends on the presence of the FH1 module (Takaishi et al., 2000).
The highly conserved proline-rich FH1 domain is held responsible for the observed interaction between mDia1 and mDia2 and SH3-module proteins, which links both diaphanous proteins to tyrosine kinase signaling and serum response factor (SRF)-dependent transcriptional activity (Alberts, 2001; Tominaga et al., 2000). Furthermore, the FH1 domains in diaphanous proteins from budding yeast, fission yeast, insects and mouse are considered to mediate binding to profilin (Chang et al., 1997; Evangelista et al., 1997; Imamura et al., 1997; Manseau et al., 1996; Watanabe et al., 1997). Profilins are involved in the regulation of actin polymerization. They bind simultaneously to G-actin and to polyproline-sequences, as contained in the FH1 domain (Schlüter et al., 1997). Targeting profilin-actin complexes to the FH1 domains might thus effectively recruit actin to specific cellular sites, and stimulate filament polymerization. For subsequent organization of stress fibers, as induced by activated Rho, RBD and FH1 domains may act in concert. In addition, for yeast diaphanous proteins, binding to actin itself and to other actin-binding proteins has been described (Evangelista et al., 1997), indicating that in these cells the physical link between diaphanous proteins and the actin cytoskeleton may not be confined to profilin-binding.
At present, the information on ligand binding and cellular function of the FH2 and FH3 domains is rather scarce. The FH2 module of mDia1 has been implicated in the coordination of microtubule and actin filament networks (Ishizaki et al., 2000), but the molecular basis of this linkage is unclear. The FH3 domain, which is less well conserved among different species and overlaps with the RBD, is essential for targeting the relevant fission yeast diaphanous protein to the polar tips of mating cells (Petersen et al., 1998). This may also be related to cytoskeleton-mediated processes of generating cell polarity. In HeLa cells, the FH3 domain was found essentially responsible for mDia1 association with the mitotic spindle (Kato et al., 2001).
When truncated mDia1 constructs lacking the RBD are transiently transfected into cells, they induce SRF-dependent nuclear transcription (Tominaga et al., 2000) and stimulate stress fiber formation (Watanabe et al., 1999), suggesting that this domain acts as a negative regulator on several levels of diaphanous activity. Autoregulation of Dia1 and Dia2 proteins has been found to involve intramolecular bonding between N- and C-terminal motifs and its release by activated Rho (Alberts, 2001; Watanabe et al., 1999). Thus, RBD-Rho complex formation has a dual function: it opens the diaphanous polypeptide and activates the entire molecule, and it stimulates site-specific Rho-dependent actin reorganization.
To further elucidate the role of different diaphanous domains in signal-dependent actin organization and its consequences on cellular motility, we have generated a catalogue of deletion fragments of mDia1 and studied their properties in detail, in vitro, in transiently and in stably transfected cells. We show that in a physiological environment, the FH1 domain of mDia1 forms complexes with endogenous profilin, and the RBD with the C-terminal intramolecular interaction domain CIID, respectively. Furthermore, we demonstrate that the RBD interacts with the endogenous mDia1 in a species-restricted manner and that overexpression of this domain causes extensive, spontaneous ruffling, loss of stress fibers and loss of directed locomotion.
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MATERIALS AND METHODS |
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Antibodies
For monitoring profilin in immunoblots and immunofluorescence, the monoclonal antibody 2H11 (Mayboroda et al., 1997) and our own polyclonal antibody against bovine profilin (Buss et al., 1992) were used. For the detection of RhoA, ezrin, endogenous and transfected mDia1, the transfected RBD-containing mDia1 fragment and transfected myc-Rac in immunoprecipitation, immunoblots and immunofluorescence experiments, respectively, the following antibodies were used: polyclonal antibodies against RhoA (Santa Cruz Biotechnology, Heidelberg, Germany) and human ezrin (gift of P. Mangeat, Montpellier, France), a polyclonal antibody against mDia1 (gift of S. Narumiya, Tokyo, Japan), a monoclonal antibody against mDia1 (Becton Dickinson, Heidelberg, Germany); and a monoclonal antibody against the myc tag (gift of B. Winter, Braunschweig, Germany). Immunoprecipitation of the epitope tagged proteins in lysates of transfected cells was performed with the monoclonal antibodies anti-flag M2 (Sigma, Deisenhofen, Germany) and 4A6, which recognizes the birch profilin tag (BiPro tag) (Rüdiger et al., 1997).
Cell culture, transfection and selection procedures
CHO cells were grown in DMEM/Hams F-12 medium (1:1 mixture), supplemented with 10% fetal bovine serum (FBS). HeLa cells were transfected by the calcium phosphate method, using 2 µg DNA/20-mm dish, whereas CHO cells were transfected using the FuGene 6 reagent (Roche Biochemicals, Mannheim, Germany) according to the manufacturers protocol. They were then incubated for at least 12 hours, rinsed three times with phosphate buffered saline (PBS) and incubated for at least 6 hours. Stable CHO derivatives were obtained by culturing CHO cells (7x104/60 mm dish) for 24 hours and then transfecting them with the calcium phosphate method with 10 µg of either the control vector encoding the green fluorescent protein (pEGFP-C2), or the vector coding for the fusion protein of EGFP and deletion fragment 1-448mDia1 (pEGFP-1-448mDia). For each construct, clones were selected after culture in geneticin containing selective medium (0.6 mg G418/ml, Life Technologies, Karlsruhe, Germany). For expression of a dominant negative Rac GTPase, the stable CHO lines were supertransfected with pcDNA3/N17Rac1.
Wounding assay
Locomotory activity was assessed by following the closure of a wound set experimentally into a dense monolayer. CHO cells and their derivatives were seeded at a density of 1x105 cells in 60 mm dishes and grown to confluency. A wound approximately 1 mm wide was obtained by scratching the monolayer with a pipette tip. Cell debris was removed by rinsing the cultures extensively with PBS, and fresh medium with or without 0.1 µg/ml colcemide (Biochrom, Berlin, Germany) was applied. Wound closure was documented by photographing the cells under phase-contrast microscopy at time points 0, 2, 4, 6, 8 and 24 hours after wounding.
Immunofluorescence
Cells were grown on glass coverslips for one day prior to fixation in 4% formaldehyde and subsequent extraction with 0.2% Triton X-100 in PBS. The samples were then incubated for 30 minutes at 37°C with the respective antibody. Goat anti-mouse or anti-rabbit IgG coupled to tetramethylrhodamine B isothiocyanate (TRITC) (Dianova, Hamburg, Germany) served as secondary antibodies and phalloidin-TRITC (Sigma) for labelling of F-actin. The cells were examined in a conventional light microscope equipped with epifluorescence (Axiophot, Zeiss, Oberkochen, Germany).
Immunoprecipitation of protein complexes after in situ crosslinking
In situ crosslinking, precipitation and analysis of protein complexes were performed essentially as described (Hüttelmaier et al., 1997; Weiss et al., 1998) with slight modifications. Transfected HeLa and CHO cells were grown on 10 cm dishes (5x106/dish), rinsed in PBS and incubated at room temperature for 30 minutes with 0.5 mM of the membrane permeant crosslinker dithio-bis(succinimidylpropionate) (DSP; Pierce, Sankt Augustin, Germany). Excess crosslinker was quenched with 0.2 M glycine. After rinsing in PBS, cells were lysed in RIPA buffer (50 mM Tris, pH 7.2, 1% (v/v) Triton X-100, 0.25% desoxycholate, 1 mM EGTA, 150 mM NaCl, 2.5 mM sodium azide, 1 µM pepstatin A, 80 µM Pefabloc SC, 0.46 µM aprotinin; 30 minutes at 4°C). Cellular material was then scraped off the dish with a rubber policeman, homogenized by pipetting and centrifuged at 15,000 g at 4°C. Then, 40 µl of a 50% slurry of protein G sepharose preblocked with 2% (w/v) BSA in RIPA buffer was added to the samples, and incubation continued under stirring at 4°C for 2 hours. The sepharose beads were collected by centrifugation, washed three times with RIPA buffer and once with PBS before the samples were boiled in SDS sample buffer containing 20% (v/v) ß-mercaptoethanol, to cleave the crosslinker. Finally, the samples were analyzed by SDS-PAGE and immunoblotting, using standard procedures.
In vitro transcription/translation
The coding regions of wt-mDia1 and its deletion fragments were cloned into the vector pET28a. The [35S]-methionine labeled proteins were synthesized by in vitro transcription/translation using the TNT-coupled reticulocyte lysate system (Promega, Heidelberg, Germany), according to the manufacturers protocol.
Affinity precipitation
For the expression of recombinant mouse PFN I and II the T7 RNA polymerase expression system (Studier and Moffatt, 1986) was used. The E. coli strain BL21 (DE3) pLysS was transformed with the expression vectors pET21c/PFN I and pMW172/PFN IIa (gift of W. Witke, Monterotondo, Italy), containing the coding regions of either mouse profilin I or profilin IIa. The expressed proteins were purified by poly-(L-proline) affinity chromatography as described previously (Giehl et al., 1994). Eluted fractions were pooled and dialyzed against 10 mM Tris/HCl pH 7.6, 2 mM CaCl2, 1.25 mM dithiothreitol (DTT). The purity of the protein preparations was judged by SDS-PAGE. Purified recombinant mouse profilins I and IIa, and BSA (control) were coupled to NHS-Sepharose 4B Fast Flow (Amersham-Pharmacia, Freiburg, Germany) with an efficiency of 10-15 µg bound protein per µl NHS-material. Free NHS-groups were blocked with ethanolamine. Aliquots of 25 µl in vitro transcription/translation reaction mixture were diluted in PBS and incubated with 10 µl conjugated sepharose for 4 hours at 4°C. After sedimentation, the beads were successively washed with PBS, PBS with 140 mM NaCl and PBS with 0.2% Triton X-100. The denatured proteins of the first supernatant and of the washed pellets were separated by SDS-PAGE and the gel was blotted onto nitrocellulose membrane. The [35S]-labeled proteins were detected by autoradiography.
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RESULTS |
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The RBD-CIID interaction is species-restricted and requires the FH3 fragment
Exogenous RBD, when transfected into cells, has been shown to bind to endogenous mDia1 and mDia2, thus activating them (Alberts, 2001; Watanabe et al., 1999). We analyzed the requirements for this interaction in two different cell lines. Human (HeLa) and hamster (CHO) cells were transiently transfected with the RBD-containing 1-448mDia1 fragment, fused to the BiPro tag. Immunoblots revealed that in both cell lines the endogenous Dia1 protein was detectable with an antibody against mammalian Dia1 (Fig. 3A, top panel) and expressed the exogenous construct, as revealed by the tag-specific antibody (Fig. 3A, center panel). Cells were then treated with DSP, and lysates were subjected to immunoprecipitation with the 4A6 antibody and SDS-PAGE. Blotting with the mDia1-specific antibody revealed that the endogenous Dia1 of the rodent (CHO) line coimmunoprecipitated with the exogenous RBD-containing fragment (Fig. 3A, bottom panel). Precipitation of CHO-Dia1 with this method was never quantitative, but consistently seen in several independent experiments, indicating that murine RBD-containing fragment can release the intramolecular bonding in hamster endogenous Dia1 and complex with its C-terminal part. However, this was strictly confined to rodent cells: the murine mDia1 fragment consistently failed to coprecipitate with hDia1 from HeLa cells (Fig. 3A, bottom panel).
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The RBD causes loss of stress fibers and induces ruffling
The effect of overexpressing RBD on the actin organization was tested in murine cells, to enable cooperation between the exogenous fragment with endogenous mDia1. NIH 3T3 cells were transiently transfected with various EGFP-equipped N-terminal deletion fragments of mDia1, and the localization of these fragments and the organization of the actin cytoskeleton were monitored by double fluorescence analysis after staining with TRITC-phalloidin. Fig. 4 shows that the fragment comprising the RBD and almost the entire FH3 domain (63-448mDia1) causes flattening of the expressing cells and loss of stress fibers. The fragment itself was found to colocalize with F-actin in prominent membrane ruffles (Fig. 4A-C). Ruffling activity was high in the transfected cells, but very low in nontransfected control cells in the same culture. By using different deletion fragments, we found that loss of stress fibers and ruffling were both dependent on the presence of RBD, but ruffling required additional RBD-flanking residues: either the N-terminal extension or the major portion of the FH3 (Fig. 4D). Identical results were obtained with either BiPro- or flag-tagged constructs and corresponding immunofluorescence to detect transfected cells. In all cases where ruffling was induced, the exogenous protein colocalized with F-actin within the ruffles.
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DISCUSSION |
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Our results imply that the hereditary progressive hearing loss, described for a family expressing a C-terminally truncated form of hDia1 is probably not due to lack of interaction between this gene product and profilin in the hair cells of the inner ear (Lynch et al., 1997). This fragment still contains the FH1 domain, and our data showing in vitro complex formation between several deletion fragments of mDia1 and profilins suggest that FH1 is apparently properly folded even in very short fragments. The FH1 module is multifunctional, as it not only binds profilins but also several SH3 proteins involved in growth regulation (Tominaga et al., 2000). This may explain the fact that we could not obtain viable cells overexpressing this domain (data not shown), but renders any analysis on the biological function of the interaction between mDia1 and profilins difficult. Binding of profilins has been described for a variety of poly-proline-cluster proteins, ranging from microfilament-associated components (Reinhard et al., 1992) to proteins involved in RNA-processing (Giesemann et al., 1999) and mRNA-localization (Zhao et al., 2001). At present, the cellular significance of these interactions are far from understood but are thought to provide the cell with profilin-actin complexes in regions where rapid actin polymerization is required or at least advantageous. This view is supported by findings that profilins stimulate VASP-associated actin-based motility of the pathogen Listeria monocytogenes (Geese et al., 2000; Laurent et al., 1999), contribute to the dynamics of cortical actin filaments as executed by the profilin-binding, proline-cluster proteins WASP and WAVE (Takenawa and Miki, 2001) and optimize actin-dependent transcription of viral RNA (Burke et al., 2000). The importance of the poly-proline-binding site on profilin for cell viability has been unambiguously demonstrated in yeast (Lu and Pollard, 2001).
In contrast to the situation with the FH1 domain, overexpression of the Rho-binding domain was tolerated by a number of cell lines, including HeLa, CHO and 3T3 cells, and we show that the RBD can interact with the endogenous mDia1 protein in transfected cells. An intact RBD is essential for this interaction, as an N-terminally truncated RBD fragment was found ineffective (data not shown). Remarkably, we found that this interaction is species-restricted (i.e. complex formation could only be observed within rodents). This might be explained by sequence variance within the RBD-binding CIID. For example, while the overall sequences between hDia1 and mDia1 are highly homologous, the C-terminal part of the CIID of both proteins displays considerable differences: the aa residues 1228-1240 read PGPVKVPKKSEGV in mDia1 versus AVPAKVSKNSETF in hDia1 (sequences for both proteins according to EMBL accession numbers U96963 and AF051782, respectively). On the other hand, the motif aa 1177-1207, N-terminally located, but still contained within the CIID and termed diaphanous autoregulatory domain, DAD, (Alberts, 2001) (Fig. 1), was found to interact with the RBD in vitro and in microinjection experiments (Alberts, 2001). This sequence is identical between mDia1 and hDia1. This apparent discrepancy might be due to the fact that in the study cited, mDia2 was examined, not mDia1. Taken together, these data and ours suggest that the DAD, a highly conserved motif, is essential for molecular recognition of the RBD, but may not be sufficient, depending on the Dia isoform or the relevant species.
Several groups have analyzed the effect of overexpressing or injecting isolated mDia domains on actin organization. An increase in RBD or RBD-containing fragments resulted in loss of stress fibers in HeLa cells (Watanabe et al., 1999), consistent with our data reported here for 3T3 and CHO lines. By contrast, an increase in DAD resulted in an increase of F-actin and stress fibers in NIH 3T3 (Alberts, 2001), and overexpression of various mDia1 fragments lacking the RBD induce stress fibers in HeLa cells (Watanabe et al., 1999) and cause a parallel arrangement of stress fibers in MDCK and HeLa cells (Ishizaki et al., 2000; Takaishi et al., 2000). Hence, it seems that unbalancing the level of the different Dia domains may shift the actin suprastructural organization to different sides. However, so far, nobody has reported the prominent ruffling activity that we see upon overexpressing the RBD in both NIH 3T3 and CHO cells. By examining three different, independently created stable clones, we found that this ruffling is indeed exhibited continuously by all cells. The ruffles are defined by their content in F-actin and ezrin, and they also contain the transfected RBD and RhoA. A colocalization of full-length mammalian Dia1, profilin and Rho has previously been described in ruffles of MDCK cells, induced by the application of 12-O-tetradecanoylphorbol-13-acetate (TPA) (Watanabe et al., 1997), but the cells described here ruffle spontaneously, without addition of external factors. An intact RBD is required for this phenomenon, as transfection with an N-terminally truncated RBD did not cause ruffling (data not shown). The ruffling cells, most of which were flatter and without a distinct morphological polarity, behaved disoriented and were handicapped in directed locomotion. Loss of stress fibers and ruffling activity were correlated in most cases, but a fragment comprising the RBD but lacking the C-terminal portion of the FH3 induced only the former. This suggests that the N-terminal part of the FH3 may positively influence ruffle formation, either by cooperating with or at least by stabilizing the RBD. By contrast, the extreme C-terminal part of the FH3 domain, in particular two leucine residues at positions 434 and 455, seem essential for targeting mDia1 to the mitotic spindle (Kato et al., 2001).
Ruffling activity is generally ascribed to the activity of Rac (Nobes and Hall, 1995; Ridley et al., 1992), and this was also confirmed for the stable clones described here: supertransfecting the stably RBD-expressing CHO cells with N17Rac1, a dominant negative Rac mutant that interferes with growth factor-dependent ruffling activity (Ridley et al., 1992), abolished ruffling completely. Consistent with this assumption, we observed that microinjection of the ADP-ribosyltransferase C3, which is a potent inhibitor of Rho (Aktories and Hall, 1989), induces ruffling in CHO control cells (data not shown). Hence, our data suggest that by overexpressing RBD, endogenous activated Rho is effectively downregulated in its activity, and, as a consequence, Rac activity is upregulated. Such feedback between Rho and Rac activity has been repeatedly reported, and while in most cases Rac was seen to regulate Rho (Zigmond, 1996), there are some recent observations that also postulate the reverse (Cox et al., 2001), corroborating our concept. Future research will have to clarify on a molecular basis the crosstalk between these two GTPases and the precise role of mDia1 in actin organization.
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ACKNOWLEDGMENTS |
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