Department of Dermatology and Cutaneous Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
* Author for correspondence (e-mail: Sirpa.Aho{at}mail.tju.edu )
Accepted 8 January 2002
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Summary |
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Key words: Catenin, Cadherin, Cell-cell adhesion, Cell morphology, Alternative splicing, Protein domains
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Introduction |
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Unlike ß-catenin, multiple p120ctn isoforms are generated through
extensive splicing and alternative usage of translation initiation codons
(Keirsebilck et al., 1998;
Mo and Reynolds, 1996
). The
Armadillo domain of p120ctn facilitates interaction with the intracellular JMD
of E-, N- and C-cadherins within the adhesion complexes
(Reynolds et al., 1996
;
Thoreson et al., 2000
).
Originally p120ctn was identified as a major substrate for tyrosine
phosphorylation (Reynolds et al.,
1989
). Recently, eight tyrosine residues within the N-terminal
domain were identified as major sites of Src-induced p120ctn phosphorylation
(Mariner et al., 2001
). The
N-terminus of p120ctn is responsible for the interaction with the cytoplasmic
tyrosine kinase FER (Kim and Wong,
1995
). Also, the RPTPµ-interaction domain of p120ctn maps to
its unique N-terminus, a region distinct from the cadherin-interacting domain
(Zondag et al., 2000
). The
p120ctn isoform 3A was identified as a main interaction partner of the
protein-tyrosine phosphatase SPH-1
(Keilhack et al., 2000
). The
N-terminus of the isoform 3 also interacts with the N-terminal globular domain
of BPAG2/BP180/type XVII collagen, a transmembrane protein primarily located
to hemidesmosomal attachment complexes in epidermal basal keratinocytes
(Aho et al., 1999
).
Small GTPases of the Rho subfamily are signaling molecules primarily
involved in remodeling of the cytoskeleton, but they also contribute to
cadherin-mediated cell adhesion (Braga,
2000). Rho, Rac and Cdc42 GTPases regulate the assembly of
multimolecular focal complexes associated with actin stress fibers,
lamellipodia and filopodia (Nobes and
Hall, 1995
). The interaction of p120ctn with Rho family GTPases
has been recently described (Anastasiadis
et al., 2000
; Grosheva et al.,
2001
; Noren et al.,
2000
). Transfection experiments have shown that p120ctn can induce
a dramatic effect on the morphology of fibroblasts
(Reynolds et al., 1996
).
Specifically, ectopic overexpression of p120ctn was associated with the
formation of cellular extensions reminiscent of filopodia and dendrites. The
intact Armadillo domain and the C-terminal domain were required for the
induction of the branching phenotype
(Reynolds et al., 1996
). Thus
p120ctn can regulate cell adhesion and motility through the actin cytoskeleton
via Rho family GTPases.
An infrequent splicing event inserts an exon C between the Armadillo
repeats 6 and 7 of p120ctn, thus disrupting a potential nuclear localization
signal (Keirsebilck et al.,
1998). Furthermore, splice variants of the extreme C-terminus of
p120ctn have been identified (Keirsebilck
et al., 1998
; Mo and Reynolds,
1996
). Murine transcripts appear either with or without a
C-terminal exon A (Mo and Reynolds,
1996
), although the exon A is regularly included in human
transcripts (Keirsebilck et al.,
1998
). A putative nuclear export signal with a characteristic
leucine-rich motif is encoded by another alternatively spliced C-terminal
exon, exon B (van Hengel et al.,
1999
).
In this study, we took advantage of the branching cell phenotype to investigate whether different p120ctn isoforms serve distinct roles in cell adhesion and motility. We show that both the N-terminal and C-terminal sequences are essential for this phenotype, as is the Arm domain. Furthermore, we provide evidence that nuclear localization of p120ctn cosegregates with the branching phenotype and that the presence of a C-terminal exon B abolishes both nuclear localization and the branching phenotype. The opposite effects of p120ctn isoforms on cellular morphology may have physiological relevance, as we demonstrate lineage-specific expression patterns in two major epithelial cell types, that is, keratinocytes and melanocytes, and disclose alterations of p120ctn expressed in tumor cells.
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Materials and Methods |
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The expression constructs for the N-terminal domains (N1, N3) were generated with the FLAG-tagged isoform-specific primers as above and with the reverse primer 5'-ATC CGA TGG CAC CTC CTC A-3', which was complementary to the N-terminal sequences of p120ctn isoform 4. For the expression studies, the PCR products were ligated into pcDNA3 vector as above. The N-terminal construct of isoform 1 (pcDNA3-N1) was further truncated by ApaI digestion and religation, which generated a cDNA encoding a polypeptide of 258 amino acids.
The C-terminally deleted isoform 1X (1A deletion XhoI) was
generated from pcDNA3-1A by XhoI digestion and religation, which
truncated the C-terminus from the seventh Armadillo repeat at amino acid 653.
The isoform 1AB was obtained by replacing an XhoI fragment from
pcDNA3-1A with an XhoI fragment containing the alternatively spliced
exon B generated by PCR with primers 5'-GTG TTT GCC TTC TTC GGA
ATC-3' and 5'-CCC AGA TGG AAC GGA GAT-3', and using the
colon adenocarcinoma CX-1 cDNA as a template (Tumor Tissue MTC Panel,
Clontech). The PCR-product was first ligated into Bluescript, from which the
XhoI fragment was separated and subcloned into XhoI-digested
pcDNA3-1A. The pcDNA3-1AB was used as a template for in situ mutagenesis using
the QuickChange Mutagenesis kit (Stratagene). The double mutant L951A, L953A
was generated as described previously (van
Hengel et al., 1999
).
An N-terminal deletion construct 2ANS (2A deletion
NaeI-SmaI) was prepared from pcDNA3-2A through deleting a
510 bp NaeI-SmaI fragment, which resulted in the deletion of
amino acids 63-232. Another N-terminal deletion construct 3A
AK (3A
deletion AvrII-KasI) was prepared from pcDNA3-3A through
deleting a 341 bp AvrII-KasI fragment. The ligation, after
filling the ends with the Klenow-fragment of DNA polymerase I, resulted in the
in-frame expression construct with amino acids 205-316 omitted. The putative
bipartite nuclear localization signal, encoded by amino acids 306-319, was
deleted from expression plasmids pcDNA3-2A and pcDNA3-3A. The plasmids were
linearized with KasI, treated with S1 nuclease and recircularized
with T4 DNA ligase. The in-frame ligations resulted in the expression
constructs pcDNA3-2A
NLS (2A deletion nuclear localization signal),
encoding isoform 2A with amino acids 310-326 deleted, and pcDNA3-3A
NLS
(3A deletion nuclear localization signal), encoding isoform 3A with amino
acids 305-325 deleted.
The N-terminal 1-kb EcoRI-fragment from Ubinuclein
(Aho et al., 2000) was joined
into the EcoRI site at the 5'-end of cDNA encoding p120ctn
isoform 4 in the expression plasmid pcDNA3-4A. The inframe fusion produced an
expression plasmid pcDNA3-4A+Ubi NLS. As the second approach, the
complementary oligonucleotides encoding three copies of SV40 bipartite nuclear
localization signal were annealed and ligated at the 5'-end of the cDNA
encoding isoform 4A, producing the expression construct pcDNA3-4A+SV40
NLS.
The cDNAs produced by PCR were sequenced and compared with the cDNA sequence in the GenBank (Accession number AF062341). DNA sequences were obtained using the PRISM Ready Reactions DyeDeoxy Terminator Cycle sequencing Kit and the Applied Biosystems Models 373A and 377 DNA sequencing systems (The Nucleic Acids Facilities, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA). The sequences were edited and analyzed using MacVector 6.0 (Oxford Molecular).
Cell cultures and transfections
Normal human keratinocytes (NHK) were derived from neonatal foreskins and
propagated in KGM medium, adjusted to 60 µM calcium, and supplemented with
growth factors (Bullet kit) as supplied by the manufacturer (Clonetics). HaCaT
keratinocyte cultures (Boukamp et al.,
1988) were maintained in the W489 medium supplemented with 2% FCS
(Rodeck et al., 1987
).
FM516Sv3/3 human immortalized melanocytes were described previously
(Melber et al., 1989
). Human
melanoma cell line 1205-Lu is a variant of WM164 cells, selected for
metastatic capacity through serial passages in nude mice
(Juhasz et al., 1993
). The
squamous carcinoma cell lines SCC12, A253, FADU and DET562 were a generous
gift from J. Rheinwald. A431 squamous carcinoma cells were obtained from ATCC
(Rockville, MD).
For the transient transfections, cells were plated on the chamber slides or on the 35 mm dishes, 1205-Lu cells for 4-6 hours and HaCaT cells for 6-8 hours. Transfections were performed using FUGENE-6 according to the manufacturer's instructions (Roche). 1205-Lu cells were further incubated in W489 medium supplemented with 2% FCS for 16 hours before preparing for immunofluorescence detection. HaCaT cells were also incubated in W489 medium supplemented with 2% FCS for 16 hours, then the serum-free KGM-medium with 1 mM Ca2+ was changed and the incubation was continued for additional 8 hours.
Before immunological detection, cells grown on the chamber slides or tissue culture dishes were washed three times with PBS (phosphate buffered saline).
Immunological detection
A FLAG-tag-specific monoclonal antibody M2 (Stratagene) was used at 1:1000
dilution for both immunoblotting and indirect immunofluorescence (IIF). A
rabbit polyclonal antibody against FLAG-tag was obtained from Abcam Ltd.
(www.abcam.com
) and used in 1:500 dilution for IIF. The pp120 monoclonal antibody, detecting
all p120ctn isoforms, was purchased from Transduction Laboratories and used in
1:2000 dilution. Monoclonal antibody 6H11 was a generous gift from A. Reynolds
(Vanderbilt University, Nashville, TN). It was used at 1 pg/ml to detect the
long p120ctn isoform 1 (Wu et al.,
1998). The E-cadherin monoclonal antibody was obtained from
Transduction Laboratories and used at a 1:1000 dilution. The species-specific
anti-mouse and anti-rabbit secondary antibodies, conjugated to FITC, Texas Red
or horseradish peroxidase were from Jackson Laboratories (West Grove, PA).
For western blotting, cells were lysed directly on the tissue culture dishes, and proteins were extracted using SDS-loading buffer (Bio-Rad). Proteins were separated by SDS-PAGE and transferred onto the PVDF membrane (New England Nuclear Life Science, Boston, MA). P120ctn polypeptides were detected with primary antibodies as indicated in the figure legends, followed by the anti-mouse HRP-conjugated secondary antibody (Jackson Laboratories, West Grove, PA), and visualized using the Renaissance western blot chemiluminescence reagent (New England Nuclear Life Science Products).
A section of human adult skin, embedded and frozen in the OCT compound, was cut into 7 µm cryosections, which were air dried and stored at -20°C. Slides with cryosections, or the chamber slides with cells, were washed three times with PBS and fixed in methanol at -20°C for 5 minutes. Cells were permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, washed three times with PBS and blocked with 1% BSA (bovine serum albumin) in PBS for 1 hour at room temperature. The primary antibodies were applied on the samples overnight at 4°C. After four washes with PBS, samples were incubated for 1 hour at room temperature with the species-specific secondary antibody conjugated to FITC or Texas Red and 0.00002% DAPI, washed four times with PBS and mounted for viewing. Sections and slides were evaluated by fluorescence microscopy (Axioskop; Carl Zeiss, Inc.), images were stored with ImagePro Plus 4.0 imaging software (Media Cybernetics) and processed with Photoshop 5.0 (Adobe Systems Inc.) and Canvas 5 (Deneba Software).
Expression studies by PCR
Human multiple tissue cDNA panels (Human MTC Panels I and II and Human
Tumor MTC Panel) were obtained from Clontech and used as templates for PCR
analysis. The p120ctn-specific primers, 5'-GAAGCCAGAGCAGTCATTCA-3'
and 5'-CCCAGATGGAACGGAGATA-3', produced a PCR fragment of 273 bp,
when exon B was not included in the template, and a fragment of 360 bp, when
exon B was present in the cDNA. PCR conditions were 2 minutes at 94°C,
followed by 38 cycles of 94°C for 20 seconds, 58°C for 30 seconds and
72°C for 1 minute. PCR was conducted using Taq DNA Polymerase and the
Q-solution provided with the kit (Qiagen). The PCR-products were separated on
1.5% agarose-TBE gels.
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Results |
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Expression of multiple p120ctn isoforms in normal and malignant
epithelial cells
To further evaluate the expression of different p120ctn isoforms in
keratinocytes and melanocytic cells, we performed immunoblot analysis using
extracts of these cells and transformed cells derived from these lineages. As
show in Fig. 2, the MAb pp120
recognized four major p120 bands in the immunoblot analysis. Consistent with
the immunohistochemical results, three major protein species, excluding
isoform 1, were identified in neonatal keratinocyte lysate (NHK). Bands 2, 3
and 4 appeared to be tightly spaced doublets, either owing to the alternative
usage of exons A, B or C or as a result of post-translational modifications.
HaCaT cells, which are immortalized but non-tumorigenic keratinocytes,
contained a similar set of isoforms. A panel of five squamous carcinoma cell
lines expressed mainly band 2 but not bands 3 and 4. Consistent with the
immunohistochemical results in Fig.
1 and in contrast to keratinocytes, immortalized melanocytes
(FM516SV3/3) and a panel of four melanoma cell lines expressed primarily the
long p120ctn isoform 1 (Fig.
2B, upper panel), although they coexpressed shorter isoforms to a
lesser degree (Fig. 2B, lower
panel). The monoclonal antibody 6H11 detected a single band corresponding to
the isoform 1 only in melanocyte and melanoma cell extracts but not in
extracts from either normal keratinocytes or HaCaT cells. This finding
confirms the notion that the expression of p120ctn isoform 1 is characteristic
of melanocytes.
|
C-terminal sequences encoded by exon B contribute to additional
p120ctn isoforms
Additional p120ctn isoforms are generated through the alternative splicing
of an exon B, which encodes a 29 amino acid peptide including a nuclear export
signal (van Hengel et al.,
1999). Our RT-PCR results indicated that exon B was not included
in p120ctn transcripts either from the epidermal derived keratinocytes or the
squamous carcinoma cells (data not shown), although it has been generally
detected in most epithelial cells (van
Hengel et al., 1999
). RT-PCR analysis of Multiple Tissue cDNA
panels demonstrated exon B sequences prominently present in p120ctn
transcripts from pancreas, prostate, colon and small intestine, whereas the
tumor tissues derived from colon (G1-112), pancreas (G1-103) and prostate
(PC-3) had lost exon-B-containing transcripts
(Fig. 3). A weak signal also
indicated the presence of exon B in normal kidney, liver, lung and placenta,
as well as in lung, breast and ovarian carcinomas
(Fig. 3).
|
N-terminal sequences of p120ctn are required for the branching cell
phenotype
The distinct expression patterns of p120ctn in epidermal cell types raised
the question of whether the observed differences in the composition of the
p120ctn N-terminus have functional consequences. To address this question we
turned to a bioassay based on the capacity of the p120ctn to induce extensive
cellular extensions when overexpressed in fibroblasts
(Reynolds et al., 1996). To
this end, FLAG-tagged expression constructs were developed: (a) p120ctn
isoforms 1AB, 1A, 2A, 3A and 4A; (b) N-terminal sequences N1, N3 and
N1
A; (c) the isoform 4A equipped with additional nuclear localization
sequences; and (d) the isoforms containing both N-terminal and C-terminal
modifications (Fig. 4) and
transiently transfected into 1205-Lu melanoma cells and immortalized
keratinocytes (HaCaT). A FLAG-tag-specific antibody was used to demonstrate by
immunoblotting that each construct was correctly expressed after transient
transfection into melanoma cells 1205-Lu
(Fig. 4C) and HaCaT cells (not
shown).
|
Similar to previously published observations in fibroblasts
(Anastasiadis et al., 2000;
Reynolds et al., 1996
),
p120ctn isoforms 1A, 2A and 3A induced prominent cellular extensions
resembling dendrites, filopodia and lamellipodia in melanocytic cells
(Fig. 5A-D). As demonstrated
with isoform 1A, the intensity of the signal showed a positive correlation
with the cellular phenotype. Lower expression of the transgene was detected
only in the cytoplasm, although in a cell with more intense staining, the
FLAG-tagged protein expressed from the transgene entirely filled the nucleus,
lamellipodia and filopodial extensions. Finally, a cell demonstrating an
extremely strong signal appeared as a rounded cell body from which
dendritic-like extensions and numerous filopodia protruded across the field of
neighboring cells (Fig. 5A,B).
The isoform 4A did not induce dendrites, filopodia or lamellipodia, and the
staining was detected exclusively in cytoplasm
(Fig. 5E,F).
|
Multiple targeting signals determine the subcellular localization of
p120ctn
The presence of a nuclear localization sequence encoded by the N-terminal
sequences of p120ctn was confirmed by using FLAG-tagged expression constructs
encoding the N-terminus of p120ctn isoform 1 (N1), isoform 3 (N3) and N1
truncated after amino-acid residue 258, deleting 88 amino acids upstream from
the Armadillo domain (N1A). Upon transfection into 1205-Lu cells, the
N1 and N3 polypeptides were observed in the nucleus
(Fig. 6A-D). The C-terminally
deleted N1 showed primarily cytoplasmic localization, although diffuse nuclear
staining was observed as well (Fig.
6E,F). Detailed examination of the amino-acid sequence within the
88 amino-acid stretch revealed a putative bipartite nuclear localization
signal at amino acids 306-319 to be responsible for the nuclear localization
of N1 and N3. The diffuse nuclear staining detected with N1
A is
probably due to the fact that small proteins (less than 40 kDa) do not possess
a specific targeting signal and can enter through the nuclear pores without a
specific nuclear localization signal.
|
Because the N-terminal sequences of p120ctn isoform 3A seemed to be
important for both nuclear localization and branching phenotype, we postulated
that the N-terminal nuclear localization signal is responsible for these
functions. However, the cellular phenotype obtained by the isoforms 2A and 3A
from which the N-terminal nuclear localization signal was deleted,
2ANLS and 3A
NLS (Fig.
7A,B), were indistinguishable from the wild-type isoforms 2A and
3A (compare with Fig. 5C,D).
The deletion of amino acid 63-232 (2A
NS)
(Fig. 7C) resulted in milder
phenotype compared with that of isoform 2A
(Fig. 5C), whereas the deletion
of amino acids 205-316 (3A
AK) abolished the nuclear localization and
the branching phenotype, although some lamellipodia were still detectable at
the lateral cell membranes (Fig.
7D).
|
Although the isoform 4A contained intact Armadillo- and C-terminal domains,
its location was found to be strictly cytoplasmic and did not confer a
distinct branching phenotype (see Fig.
5E,F). To test the hypothesis that bringing the isoform 4A into
the nucleus would induce the branching phenotype, three copies of the SV40
nuclear localization sequences were attached N-terminally to the p120ctn
isoform 4A. The corresponding protein (4A+SV40 NLS;
Fig. 7E,G) localized both in
the nucleus and in the cytoplasm, but it did not induce the branching
phenotype. Also, when the N-terminus of ubinuclein was attached to the p120ctn
isoform 4A, the fusion protein (4A+Ubi NLS;
Fig. 7F,H) was detected in the
nuclei but no branching phenotype was induced. These results showed that
neither the previously characterized nuclear localization sequence within the
Armadillo-repeat region, between the repeats 6 and 7, nor the putative nuclear
localization sequence within the N-terminus are sufficient or necessary for
the nuclear localization. Instead, the critical region for nuclear
localization is located between amino acids 233 and 303, the region that
contains five out of eight tyrosine residues phosphorylated by Src in p120ctn
(Mariner et al., 2001).
Previously, a nuclear export signal has been identified in human exon B of
p120ctn (van Hengel et al.,
1999). Consistent with this earlier report, exon B, when included
in an expression construct encoding the p120ctn isoform 1A, led to nuclear
exclusion of the transgene (isoform 1AB;
Fig. 7I). Importantly, the
p120ctn isoform 1AB did not induce cellular branching, whereas some flattened
cells with lamellipodia were detected. This is consistent with the observation
of nuclear localization cosegregating with the branching phenotype. When the
nuclear export signal was altered through site-directed mutagenesis as
described previously (van Hengel et al.,
1999
), a slight change in the cellular phenotype was detected
(1ABm, Fig. 7J), but the
striking branching phenotype of isoform 1A was not observed.
Cells transfected with p120ctn isoform 1A displayed a spectrum of
phenotypes, suggesting that the branching phenotype develops gradually
(Fig. 8A-D). Although some
cells displayed the normal 1205-Lu phenotype, flattened cells with
lamellipodia and filopodial extensions were also detected. The percentage of
cells displaying long dendrites with multiple branches was time dependent
(data not shown). When 1205-Lu cells were harvested for immunofluorescence 24
hours after transfection, a considerable number of cells expressing isoform 1A
was detached, and only traces of branches could be detected on the slide. The
population analysis of the 1205-Lu cells 16 hours after transfection with
isoforms 1A, 2A and 3A revealed that 20-25% of transfected cells showed normal
phenotype, and the rest of the transfected cells displayed the altered cell
phenotype (Fig. 8E). As shown
in Fig. 5 (E,F), the isoform 4A
completely lacked the ability to induce a phenotypic change. Also, when the
exon-B-encoded sequences were included in the p120ctn, the isoform 1AB lacked
the ability to induce a branching phenotype, and only about 10% of cells
developed lamellipodial and filopodial protrusions. As shown in
Fig. 7 (A,B), the deletion of
the putative nuclear localization signal immediately upstream from the
Armadillo domain did not abolish completely the branching phenotype but
resulted in a switch towards a milder phenotype, especially with isoform
3ANLS became evident (Fig.
8E). Further deletions within the N-termini of isoforms 2A and 3A
resulted in 84% and 95% of cells, respectively, displaying milder phenotypes,
as exemplified in categories A and B (Fig.
8).
|
Overexpression of p120ctn in HaCaT keratinocytes interferes with
E-cadherin expression disrupting the cell-cell adhesion
In the epithelial cells, p120ctn binds to the juxtamembrane region of
E-cadherin and thus localizes to the cell membrane in a
Ca2+-dependent manner. Keratinocytes primarily expressed shorter
p120ctn isoforms, especially isoform 3A, which is known as an epithelial
p120ctn isoform. Surprisingly, all three isoforms, 1A, 2A and 3A, and also the
deletion construct 2ANS, colocalized with E-cadherin at the cell-cell
junctions (Fig. 9B,J,P,K), but
the overexpression of these transgenes downregulated the E-cadherin signal
both in the cytoplasmic pool and at the cell-cell junctions
(Fig. 9F,M,T,N). HaCaT cells,
expressing high levels of these isoforms, lost the cell-cell contact and
demonstrated the prominent branching phenotype
(Fig. 9A,I,O), loosing the
E-cadherin signal as well (Fig.
9E,L,S). This event is clearly demonstrated in
Fig. 9, panels K and N, where a
cell in the process of loosing the cell-cell contact on one side is negative
for E-cadherin but on the opposite side still demonstrates E-cadherin staining
along the cell-cell contact regions.
|
Isoforms 4A (Fig. 9R) and
1AB (Fig. 9C), as well as the
deletion construct 3AAK (Fig.
9Q), were detected only in the cytoplasmic pool and did not
interfere with cell-cell contact and did not demonstrate visible effects on
E-cadherin expression level (Fig.
9V,G,U, respectively). The C-terminally truncated p120ctn isoform
1 showed both nuclear and cytoplasmic staining
(Fig. 9D) and did not interfere
with E-cadherin subcellular localization
(Fig. 9H).
Collectively, these results revealed that the N-terminal amino acids 233-303, the Armadillo-repeat region and the C-terminus devoid of exon B are necessary for p120ctn to interact with E-cadherin are necessary for p120ctn to interact with E-cadherin. Isoforms 1A and 2A, as well as the epithelial isoform 3A, when expressed at high levels, can either downregulate E-cadherin expression or relocate it from the cell-cell junctions, thus destabilizing cell-cell adhesion and resulting in the prominent branching phenotype.
Nuclear localization cosegregates with the capacity of p120ctn
constructs to induce a branching phenotype
While monitoring the expression of the p120ctn constructs in HaCaT cells,
we noticed distinct subcellular distribution patterns for the FLAG-tagged
proteins. In each case they accumulated in the cytoplasm. However, as shown in
Fig. 10, only those transgenes
(1A, 2A, 2ANS and 3A) that were able to induce the branching cellular
phenotype were present in the nuclei of the transiently transfected cells
(Fig. 10A,D,E,F). By contrast,
p120ctn isoforms 4A and 1AB, as well as 3A
AK, were excluded from the
nucleus upon transfection into either HaCaT cells
(Fig. 10H,B,G) or melanoma
cells (Fig. 5E;
Fig. 7I,D) and did not possess
a capacity to induce the branching phenotype in either cell type. Construct
1
X (Fig. 10C), with a
C-terminal deletion, but with intact nuclear localization signals both in the
N-terminus and within the Arm-domain, extensively accumulated into the nucleus
but was not able to induce a branching cellular phenotype.
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![]() |
Discussion |
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Upon cloning the human p120ctn gene, Keirsebilck et al.
(Keirsebilck et al., 1998)
recognized that different cell types contain multiple p120ctn transcripts
because of alternative splicing and translation start codon usage. These
authors concluded that theoretically 32 different p120ctn isoforms may exist
and provided RT-PCR evidence for the expression of 11 distinct p120ctn mRNA
species in various cell lines derived from epithelial and mesenchymal tissues.
They also noted that much of the heterogeneity of p120ctn isoforms is due to
N-termini of different length, which results from the usage of alternative
start codons. In our earlier work, we discovered that the splicing pattern of
p120ctn transcripts is unique for each human tissue and was consistent between
each fetal and adult tissue but was random in the tumor tissues regardless of
their origin (Aho et al.,
1999
).
Utilizing the monoclonal antibody specific for the isoform 1, we recently
reported that the long isoform 1 is specifically expressed at the
vascular-endothelial junctions in blood vessels, at the cell-cell junctions in
the serosal epithelium lining the internal organs, in the choroid plexus of
brain, in the pigment epithelium of retina and in the structures such as the
outer limiting membrane of retina and intercalated discs of cardiomyocytes
(Montonen et al., 2001). In
those tissues either VE-cadherin or N-cadherin are the major adhesion
molecules. The analysis of p120ctn isoforms in a variety of murine cell lines
has revealed that motile cells, such as fibroblasts and macrophages,
preferentially express the long isoform, and epithelial cells preferentially
express the short isoform, whereas non-adherent cells do not express
detectable levels of p120ctn (Mo and
Reynolds, 1996
). The cell-lineage-specific patterns of p120ctn
isoform expression emerged also during this investigation. Specifically,
isoform 1 is prominently expressed in melanocytic cells but not in normal or
malignant keratinocytes. In normal human skin, E-cadherin is expressed on the
surfaces of all epidermal cells, including keratinocytes and melanocytes, but
is lost from melanoma cells, which during tumorigenesis switch to express
N-cadherin (see references within Herlyn et al.
(Herlyn et al., 2000
)). These
results suggest that the tissue- and cell-type-specific expression of p120ctn
isoform 1A does not directly correlate with certain cadherin type, but the
functional difference between the isoforms 1A and 3A, perhaps in signal
transduction, remains to be studied.
We observed shifts in the expression pattern of p120ctn between normal
keratinocytes and their counterparts with malignant potential. Specifically,
the latter cells generally retained expression of band 2 but not of bands 3
and 4, which were expressed robustly by normal keratinocytes. Upon western
blot analysis, the isoform 1A could be detected with MAb 6H11, which
specifically recognize the N-terminal region of 1A. Other isoforms were
identified on the basis of their molecular size and comigration with the
transiently expressed tagged proteins. Thus band 4 represents isoform 4 and
band 3 the epithelial isoform 3, but band 2 represents either isoform 2,
translated from Met-55, or the phosphorylated form of isoform 3. The
cell-type-specific phosphorylation, although not addressed in this study, may
thus be responsible for the band shift detected through the western blotting.
The activation of protein kinase C in epithelial cells results in
dephosphorylation of serine and threonine residues of p120ctn
(Ratcliffe et al., 1997). The
disruption of tight junctions and the increase in permeability of cell
monolayers was accompanied by the dephosphorylation event. However, in
v-Src-transfected L cells tyrosine phosphorylation of p120ctn was dependent on
its association with E-cadherin and resulted in reduced cell adhesion
(Ozawa and Ohkubo, 2001
). On
the other hand, in differentiating keratinocytes, tyrosine phosphorylation
plays a positive role in the control of cell adhesion
(Calautti et al., 1998
). In
regard to our observation of the slower migrating band in the carcinoma cells,
which usually are more motile than their normal counterparts, the higher
phosphorylation level of p120ctn would correlate with decreased cell adhesion.
We also observed that the isoform 4A, although present in normal cells, was
lost in tumor cells.
Previous work has demonstrated that the overexpression of p120ctn isoform
1A in both fibroblasts and in epithelial cells caused the formation of
extensive cellular extensions (Anastasiadis
et al., 2000; Noren et al.,
2000
; Reynolds et al.,
1996
). We observed that this effect of p120ctn 1A extends to
epidermal keratinocytes and cells of melanocytic origin. In the earlier work,
Reynolds et al. (Reynolds et al.,
1996
) showed that deletion of amino acids 28-233 of p120ctn did
not affect the branching phenotype, but the expression construct with the
deletion of amino acids 233-387 was not able to induce the branching
phenotype. Because the Armadillo domain starts from amino acid 347, and
additional deletions within the Armadillo-repeat domain abolished the
phenotypic effect, it was concluded that the intact Armadillo domain is
indispensable for the branching phenotype. While investigating the
isoform-specific phenotype, we found that the isoform 4A clearly did not
induce the branching phenotype. Thus, N-terminal sequences of p120ctn impart
distinct functional properties to p120ctn, at least as measured by the changes
in cell shape after forced expression. The N-terminal domain, immediately
preceding the N-terminus of isoform 4 (amino acids 233-323) contains five out
of eight Src phosphorylation sites in the p120ctn
(Mariner et al., 2001
). As the
catalytic activity of the Src family kinases is required to disrupt
cadherin-dependent cell-cell contacts
(Owens et al., 2000
), the
phosphorylation of tyrosine residues 257, 280, 291, 296 and 302, and also to a
lesser extent tyrosine of residues 112 and 228, may play a role in the
induction of the branching phenotype by p120ctn. Colon carcinoma cells Colo
205 show defective cell adhesion, possibly owing to the aberrant p 120ctn
phosphorylation. The overexpression of p120ctn with deletion of amino acids
245-324 appeared to compete with the endogenous p120ctn, abolishing the defect
and inducing cell aggregation (Aono et al.,
1999
).
The requirement for N-terminal sequences outside of the Armadillo repeat
domains for the branching phenotype is novel, as previous studies have focused
on sequences within the Arm repeat domain as a prerequisite for branching.
Mutational analysis unequivocally demonstrated that the Arm repeat domain is
essential for branching to occur in fibroblasts, as the double point mutation
(KK/AA) substitution of residues 622-623 contained in Arm repeat 6 abolished
the induction of the branching phenotype
(Anastasiadis et al.,
2000).
The N-terminal end of the isoform 1A contains a coiled-coil region, which
is highly homologous with other Armadillo family members
(Anastasiadis and Reynolds,
2000). This coiled-coil sequence appears not to be critical for
branching because the p120ctn 2A and 3A were also capable of inducing this
phenotype. Nevertheless, the function of the coiled-coil domain remains open
to discussion and cannot be linked to the branching phenotype. The
keratinocytes form stable cell-cell adhesion complexes throughout the layers
of stratified epithelia. However, they mainly express the p120ctn isoform 3A,
which is as potent inducer of the branching phenotype as the isoform 1A. This
leads us to the conclusion that either the expression levels of p120ctn or
other p120ctn isoforms play an important role in balancing and regulating
stable cell adhesions.
Recent studies have elucidated a molecular mechanism required for the
branching phenotype to occur. Specifically, three independent reports
described that p120ctn inhibits RhoA activity, an effect that appears to be
due to p120ctn binding to the Rho family exchange factor Vav2
(Anastasiadis et al., 2000;
Grosheva et al., 2001
;
Noren et al., 2000
). At least
two distinct nuclear localization signals and one nuclear export signal have
been reported to contribute to the subcellular localization of p120ctn. Our
results indicate that the putative nuclear localization signal in Arm repeat 6
is not sufficient for nuclear localization, as the isoform 4A showed
cytoplasmic but not nuclear localization. A second putative nuclear
localization signal, located immediately upstream from the Armadillo domain,
was functional in directing the N-terminal domain of p120ctn into the nucleus,
but deletion of this sequence from isoforms 2A and 3A resulted in a less
prominent branching phenotype. However, deletion of the N-terminal sequences
containing the tyrosine residues phosphorylated by Src
(Mariner et al., 2001
)
abolished both the nuclear localization and the branching phenotype.
Conversely, if the transgenic proteins were excluded from the nuclei no
branching was observed. The presence of exon B containing a nuclear export
signal, as in p120ctn 1AB, resulted in nuclear exclusion and lack of branching
phenotype despite the presence of all sequences necessary for branching to
occur (long N-terminus, Arm repeat domain, C-terminus). Although these results
do not provide understanding of the precise molecular mechanisms leading to
the branching phenotype, they do provide a strong argument in support of the
notion that nuclear accumulation not only cosegregates with the branching
phenotype but is also essential for it.
We demonstrated the loss of the isoform 4A from tumor cells although it is present in normal keratinocytes. Although exon B was not present in epidermal cells or tumor cells of epidermal origin (data not shown), its presence in several internal organs with a prominent epithelial component was confirmed by RT-PCR. Because the expression pattern of p120ctn isoforms is characteristic to each tissue or cell type, the loss of isoform 1AB from tumors of the colon, pancreas and prostate may be significant, although it remains to be confirmed with additional material. The opposing effects observed for p120ctn in cell adhesion and mobility can be explained through the isoform-specific effects. Our results clearly support a tumor-suppressor role for the p120ctn isoforms 4A and 1AB, and the isoforms capable of nuclear localization may be considered as oncogenes, at least under certain circumstances.
In conclusion, p120ctn isoforms display different functions. The combination of p120ctn isoforms expressed by a single cell, their expression levels, and the cell-type-specific proteins interacting with p120ctn isoforms all potentially contribute to the cellular phenotype.
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References |
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