1 Department of Pathology & Laboratory Medicine, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
2 Howard Hughes Medical Institute, Department of Physiology & Biophysics, University of Iowa College of Medicine, Iowa City, IA 52242, USA
* Author for correspondence (e-mail: yurchenc{at}umdnj.edu)
Accepted 2 October 2003
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Summary |
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Key words: Dystroglycan, Costamere, Muscular dystrophy, Basement membrane
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Introduction |
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An important class of human congenital muscular dystrophy has been found to be caused by point mutations, deletions or complete absence of the laminin 2 subunit (reviewed by Miyagoe-Suzuki et al., 2000
). Several mouse strains containing
2 mutations serve as animal models for the disease (Kuang et al., 1998
; Miyagoe et al., 1997
; Sunada et al., 1995
). Among these, the murine dy2J dystrophy has been shown to result from an in-frame deletion within the N-terminal globular domain of the laminin
2 subunit (Sunada et al., 1995
; Xu et al., 1994
) that destabilizes the domain and renders it unable to polymerize (Colognato and Yurchenco, 1999
; Guo et al., 2003
). The dystrophy is characterized by degeneration and regeneration of muscle fibers. Modestly decreased levels of laminin
2 have been detected by immunofluorescence in the sarcolemmal surfaces of these mice, with irregular attenuations of the basement membrane seen at the ultrastructural level (Colognato and Yurchenco, 2000
; Sunada et al., 1995
; Xu et al., 1994
). Similarly, variable loss of the
2 subunit, near-normal levels of type IV collagen, and compensation by other laminin
-subunits have been appreciated in other laminin-
2 deficient states (Kuang et al., 1998
; Patton et al., 1999
; Sunada et al., 1994
).
Mature skeletal muscle fibers possess a rectilinear framework called the costamere, which is thought to be responsible for linking the tightly packed and aligned contractile myofibers to the sarcolemma and basement membrane (Bloch et al., 2002). The costameric framework consists of major repeating circumferential bands of receptors and cytoskeletal components that are aligned with the myofibril Z-line (Z-elements), minor repeating circumferential lines interspersed between the Z-elements (M-elements), and parallel longitudinal striations (L-elements). Vinculin, spectrin, ß1-integrin, dystroglycan and laminins have all been found to be concentrated at, or largely confined to, the elements of the costameric array, suggesting that these components are all connected to each other and linked to the myofibers Z- and M-discs by desmin and other proteins. The array has been found to be a dynamic structure whose major axis of alignment is sensitive to denervation and muscle agrin (Bezakova and Lomo, 2001
).
Recently, it was found that 1- and
2-laminins were found to organize dystroglycan, integrin
7ß1, vinculin and dystrophin into irregular polygonal arrays in cultured myotubes produced by the fusion of mouse satellite cell-derived C2C12 myoblasts, possibly constituting precursor structures to the rectilinear costameres seen in adult muscle (Colognato et al., 1999
). The changes produced by the addition of exogenous laminins to the culture medium were found to require both its polymerization and G-domain-mediated cell-surface binding. Because the LN domain of laminin that is involved in polymerization is defective in the dy2J mouse, because laminin extracted from dy2J is unable to induce the polygonal array in myotubes (Colognato et al., 1999
; Colognato and Yurchenco, 1999
), and because the same components organized by laminin into the C2C12 polygonal array are also found in costameric arrays, it seemed that a defect in the costameric array might be found in the skeletal muscle of the dy2J mouse. We therefore examined the surface distribution of basement membrane, cell surface and several cytoskeletal components in normal and dy2J mouse muscle. We report that basement membrane, receptor and cortical cytoskeletal lattice patterns appear initially normal in dy2J muscle but become distorted and effaced by 11 weeks of age. Our findings suggest that although
2-laminins contribute to the stability of the array, they are not uniquely essential for its formation.
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Materials and Methods |
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Preparation and immunostaining of skeletal muscle
Breeding pairs of heterozygous C57BL/6J-Lama 2dy-2J mice and homozygous C57BL/10ScSn mdx/mdx mice (4-6 weeks of age) were obtained from Jackson Laboratory (Bar Harbor, ME) and housed in the medical school vivarium and treated under an animal protocol approved by the Institutional Animal Care and Use Committee. Homozygous dy2J/dy2J offspring and their normal littermates were distinguished from each other by the presence or absence of a distinct gait abnormality that primarily affects the hind limbs. Following anesthetization of mice injected intraperitoneally with 0.05 ml of a 6.5% solution of sodium pentobarbital, the chest cavity was exposed, the left ventricle of the heart cannulated with a 25 gauge `butterfly' syringe needle, the right atrium incised and the animal perfused for several minutes with phosphate buffered saline (10 mM sodium phosphate, 127 mM NaCl, pH 7.4) followed by a 1.5-1.8% solution of paraformaldehyde in phosphate buffered saline for 20 minutes. Hind limb muscles were exposed by dissection and prepared for microscopy following teasing, or, for a limited number of samples, cryostat sectioning. Excised portions of muscle were slowly teased with fine forceps into individual fibers and fiber groups and adhered onto glass slides. For cryostat sectioning, a portion of muscle was rapidly plunge-frozen in a propane slush cooled to liquid nitrogen temperatures using a pneumatic-driven plunger. Sections 10-20 µm thick were cut in a Leica CM1850 cryostat at -18°C. Slides were post-fixed in 1.5% paraformaldehyde in phosphate buffered saline for 10-20 minutes. Following fixation, slides were either used detergent-free or treated with 0.5% Triton-X-100 in phosphate buffered saline for 30 minutes on ice. They were then blocked with 0.5% bovine serum albumin in phosphate buffered saline at 5°C, incubated with primary antibodies (anti-laminin-2G or anti-laminin-2/-4, 20 µg/ml; anti-type IV collagen, perlecan or nidogen, 10 µg/ml, anti-dystroglycan IIH6 conditioned medium, undiluted or 1:1 dilution; anti-integrin ß1D conditioned medium without dilution; anti-vinculin and anti-dystrophin, 1:20 dilution; anti-
-actinin, 1:400 dilution) diluted in blocking buffer. After washing, the slides were incubated with secondary antibody conjugated to FITC, Cy3 or Cy5 according to the manufacturer's instructions for 1.5 hours at room temperature. Nonimmune mouse IgM, mouse IgG and rabbit IgG were used as controls. The slides were again washed over a 2 hour period. Nuclei were stained with a 10 µg/ml solution of propidium iodide (viewed with a Chroma narrow X HQ TRITC filter set) or stained with 2 µg/ml DAPI. The latter was found to provide better tissue penetration. Coverslips were mounted with 6% 1,4 diazabicyclo(2,2,2)octane (Sigma) in a 9:1 mixture of glycerol:100 mM Tris-HCl, pH 8.6 to inhibit photobleaching.
Light microscopy and analysis of digital images
Digital confocal images were obtained with either a Zeiss LSM 410 Invert laser scanning microscope fitted with a 63x oil immersion objective (NA=1.4) and capable of discriminating among FITC, TRITC/Cy3 and Cy5 fluorescence, or an Olympus IX81 inverted microscope fitted with a CARV confocal imaging device, PCO SensiCam CCD camera, 60x oil immersion objective (NA=1.4) and DAPI, FITC, TRITC/Cy3 and Cy5 filters and controlled by IPLab (Scanalytics, Fairfax, VA). Slides were also viewed by widefield indirect immunofluorescence using an Olympus IX70 inverted microscope fitted with IX-FLA fluorescence observation attachment, 60x oil immersion objective (NA=1.4), and a MicroMax 5 mHz CCD camera (Princeton Instruments, NJ) controlled by IP Lab. For these images, out-of-focus haze was reduced by digital deconvolution of sets of 16, 32 or 64 serial optical sections recorded at 0.25 µm intervals using an expectation maximization algorithm and a calculated point spread function (Conchello and McNally, 1996; McNally et al., 1999
) in the program XCOSM (Institute for Biomedical Computing, Washington University, St Louis, MO). Confocal images were processed in Adobe Photoshop (San Jose, CA) and deconvoluted images in IP Labs and, when required, analyzed in three dimensions using Voxblast 3.0 (VayTek, Fairfield, IO).
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Results |
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Strikingly, the rectilinear laminin pattern colocalized with a similar but generally more contrasted array of -dystroglycan in both its major circumferential and longitudinal striations. Many laminin and dystroglycan costained or independently stained images also revealed the existence of finer (minor) circumferential lines lying between the major striations in the laminin-stained images, corresponding to more prominent inter-Z striations in the dystroglycan-stained images. The Z-line of the laminin and dystroglycan array was established with antibody to
-actinin. Peripheral nuclei, scattered on the myotube surface, were identified with propidium iodide and DAPI (see below), often projected beyond the sarcolemmal plane. Oblique views and serial sections revealed that the laminin and dystroglycan extended over the outer surface of the nuclei while
-actinin, marking the sarcomere, lay beneath. The laminin and dystroglycan costameric patterns in teased fibers were examined in quadraceps femoris and soleus at 6.5 weeks, and quadraceps femoris, adductor magnus, gracilis, soleus and tibialis anterior muscle at 14.5 weeks. The laminin and dystroglycan patterns were found to be continuous or near-continuous in all of these muscles, with about the same degree of variability within a muscle as between them. As shown ahead, basement membrane type IV collagen, nidogen and perlecan were also condensed into a rectilinear pattern. It was concluded that laminin-2 and laminin-4 and other major basement membrane components form a continuous sarcolemma coating but are condensed within an rectilinear array that coincides with that of dystroglycan.
Sarcolemmal surface architecture of laminin-deficient dystrophic skeletal muscle
Skeletal muscle obtained from dy2J mice aged 11-12 weeks was examined. The distribution of laminin-2 and dystroglycan was found to be substantially altered in most fibers (Figs 2, 3, 4, 5). Although a faint residual costameric pattern could often still be appreciated, the laminin and dystroglycan components were now seen to splay beyond the confines of the lattice, obscuring the normal grid-like pattern and creating a fairly diffuse and sometimes disorganized distribution of epitope (Fig. 2), i.e. the defective fibers possessed varying degrees of effacement of the lattice array pattern both for dystroglycan and laminin. Type IV collagen, nidogen and perlecan were found to possess a surface pattern in normal mouse muscle similar to that for laminin (Fig. 3). This pattern colocalized with that of ß1D integrin receptor, and cytoskeletal dystrophin and vinculin. This pattern was noted to be effaced in dy2J muscle for all of these components. By contrast, the pattern of sarcomeric
-actinin was found to remain unchanged with preservation of the evenly spaced parallel Z-bands.
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Temporal progression of surface architecture in dy2J dystrophic muscle
Muscles obtained from younger normal and dy2J mice (3 weeks, not shown, and 6.5 weeks) were evaluated. The dystroglycan and laminin costameric patterns could not be distinguished from muscle obtained from normal littermates. To quantitate the degree of surface alteration at two ages, muscle fibers were then collected from dystrophic mice aged 6.5 weeks and 11 weeks, and from their normal littermates, and evaluated by confocal microscopy. Gradations of structural abnormality could be appreciated and these were divided into three groups (Fig. 4). Class 1 fibers were defined as fibers with regular lattice patterns, sometimes with a relatively small degree of ligand staining detected beyond the fine rectilinear array. Class 2 fibers were defined as fibers with surfaces characterized by splaying of the pattern accompanied by rounding of the intersections, filament irregularity and/or a piecemeal loss of interlattice segments (particularly the M-line and longitudinal filaments). Class 3 fibers were defined as fibers characterized by extensive loss of the lattice pattern. Some of these surfaces showed preservation of central interlattice clearing, whereas others showed near-complete effacement. As shown (Fig. 5), there was little or no difference seen between normal and dystrophic mice at 6.5 weeks. The normal pattern consisted of 90% regular orthogonal array. However, profound differences in fiber architecture became evident by 11 weeks of age, i.e. 71% of fiber fields had class 2-3 effacement patterns.
The fraction of central nuclei, an indicator of regenerating and regenerated fibers, was estimated from counts of hematoxylin-stained nuclei and eosin counterstained cross-sections of contralateral hindlimb muscle. They were found to be 2% of normal 6.5-week-old muscle (74/3173 nuclei), 6% of dy2J/dy2J 6.5-week muscle (250/4254), 1% of normal 11-week-old muscle (108/10767) and 21% of dy2J/dy2J 11-week muscle (1957/9542). Increased variation in fiber diameter was noted at 11 weeks. The basement membrane laminin and dystroglycan patterns appeared essentially normal at 6.5 weeks and markedly abnormal at 11 weeks, with the fraction of affected fibers exceeding the fraction containing central nuclei. Both thin and thick 11-week-old fibers were noted to have altered surface architectures. To determine whether the costameric alterations developed in regenerated muscle fibers that contain central nuclei, and `primary' fibers that contain peripheral nuclei, 11-week-old dystrophic fibers and fiber groups were stained with DAPI to detect nuclei and immunostained with dystroglycan-specific and laminin-2/4-specific antibodies to determine the costameric pattern (Fig. 6). Serial optical sections were used to generate en face views, and orthogonal z-sections were computationally obtained from these. Fibers containing both peripheral and central nuclei were identified. The surface of both fiber types showed effacement of the normal costameric pattern. Collectively, these data suggest that the fibers that present initially at birth, as well as regenerating myofibers, had been affected.
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Sarcolemmal surface architecture of dystrophin-deficient skeletal muscle
The pattern of laminin and -dystroglycan on fibers teased from 11-week-old homozygous mdx mice was compared with age-matched controls (Fig. 7). The dystrophic fibers exhibited an altered costameric dystroglycan pattern in which the minor circumferential bands (corresponding to the M-line) and longitudinal striations were reduced in intensity, absent, or irregular, similar to those reported for spectrin in older skeletal muscle of the mdx mouse (Williams and Bloch, 1999
). The circumferential M-line and longitudinal striations of the laminin pattern were reduced compared with normal in areas overlying abnormal dystrophic striations.
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Discussion |
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Previously, we found that laminin-1 and laminin-2 can organize dystroglycan, integrin, dystrophin and vinculin into an irregular polygonal array in cultured myotubes (Colognato et al., 1999), the same components found in the rectilinear costameric framework. Although it is not known whether this irregular polygonal array is a precursor to the costamere, the organization of shared components into distinct surface architectures argues for a relationship between the two entities. One possibility is that the irregular polygonal architecture of cultured myotubes is not organized in a rectilinear array simply because the sarcomeres are too sparse and too well separated from the plasma membrane to allow components such as desmin to effectively form a bridge between the sarcomeric Z-disc and sarcolemma, as is thought to be the case in mature muscle (Bloch et al., 2002
).
It has been proposed that the costameric arrays are the sites of force transmission and that these forces stabilize membrane and cytoskeletal costameric components (Danowski et al., 1992; Sharp et al., 1997
). Because a basement membrane forms a coat around each myotube with fusion occurring between adjacent fibers, mechanical forces transmitted between myotubes during contraction must be transmitted across the adjacent basement membranes through the costameric portion. Defects arising in the array appear to be deleterious for muscle function; one such change has been evident in the mdx mouse, a model for muscular dystrophies with defects in dystrophin in which partial compensation is provided by utrophin (Grady et al., 1997
). In the mdx mouse, the array defect is characterized by a loss of spectrin and other components over M lines and in the longitudinal strands of the lattice array (Williams and Bloch, 1999
), and we found a similar pattern for dystroglycan in these mice as early as 11 weeks of age.
In the dy2J mouse, the 2-laminin subunit is deleted within the LN domain (Sunada et al., 1995
). Although defective in self-assembly, the
2-laminins nonetheless remain within basement membranes, probably tethered through nidogen, agrin, integrin, dystroglycan and other cell-surface binding molecules (Colognato and Yurchenco, 1999
). The defective laminins, unlike native laminin-1 or laminin-2, were unable to induce a polygonal array of receptors and cytoskeleton in cultured myotubes, which suggests that laminins play an important role in their surface organization (Colognato et al., 1999
). 11-week-old dy2J dystrophic muscle fibers exhibited an effaced pattern of laminin, receptors and cytoskeletal elements reminiscent of the nonpolymerization state seen on cultured myotubes after treatment with dy2J laminin; however, the normal rectilinear array organization seen in younger mice showed that
2-laminin polymerization cannot be uniquely essential for costameric assembly. Instead, the data argued that
2-laminin polymerization is important for the maintaining the stability of these structures.
However, the broader question of whether a laminin is required for costameric assembly remains unresolved. Although the 2 subunit is the major muscle laminin subunit present after birth (Kuang et al., 1999
), developing and regenerating skeletal muscle express other laminin
-subunits that associate with the ß and
subunits (Patton et al., 1999
). In particular, it has been reported in the severe laminin
2-deficient dy/dy mouse that both the laminin
4 and
5 chains are expressed at increased levels during development and regeneration, with increase of the former seen under a variety of pathological conditions (Patton et al., 1999
; Ringelmann et al., 1999
). Thus, laminin subunit redundancy and compensation exists and may insure a role of laminin in costameric assembly in either the absence of
2 expression, or the expression of defective
2 subunit. A hypothesis to be considered is that in the presence of partial laminin compensation, a lattice structural defect develops early in the dystrophic muscle, which affects stability, but which is morphologically latent. Progression to the morphological defect might require an initiating event that overstresses the weak lattice such as contraction against load, as has been proposed for non-laminin dystrophies (Campbell, 1995
).
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Acknowledgments |
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