Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky College of Medicine, Lexington, KY 40536, USA
* Author for correspondence (e-mail: sinai{at}uky.edu)
Accepted 22 September 2005
![]() |
Summary |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Key words: Toxoplasma gondii, IKK, IB, NF-
B
![]() |
Introduction |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
It is well recognized that microbial pathogens have evolved diverse strategies to manipulate the NF-B pathway (Tato and Hunter, 2002
). Inhibition of NF-
B activation has the potential of interfering with the development of antimicrobial immune responses, thus providing a survival advantage to the infectious agent. Additionally, activation of NF-
B might upregulate expression of anti-apoptotic genes and prevent death of infected cells in order to allow replication of the pathogen. The obligate intracellular protozoan Toxoplasma gondii has recently become a model organism for the study of mechanisms involved in the subversion of NF-
B and apoptotic pathways (Denkers et al., 2004
; Mason et al., 2004a
; Sinai et al., 2004
). The rapidly growing stage of the parasite known as the tachyzoite develops within a parasitophorous vacuole (PV) in the infected cell (Tenter et al., 2000
). Studies from our laboratory have shown that phosphorylated I
B
localizes to the PV membrane (PVM) surrounding the PV in T. gondii-infected cells (Molestina et al., 2003
). In addition, infected cells exhibit nuclear translocation of p50 and p65 (RelA), which correlates with the induction of anti-apoptotic genes, such as members of the Bcl-2 family and inhibitor of apoptosis proteins (IAP) (Molestina et al., 2003
). These events probably underlie the mechanisms involved in the resistance of T. gondii-infected cells to apoptotic stimuli. Accordingly, infection of p65/ (RelA/) mouse embryonic fibroblasts (MEFs) results in a loss of the anti-apoptotic phenotype and a decrease in pro-survival gene expression (Molestina et al., 2003
; Payne et al., 2003
).
The IKK complex comprises two catalytic subunits, IKK and IKKß, and a regulatory subunit, IKK
(NEMO) (Ghosh and Karin, 2002
; Hayden and Ghosh, 2004
). Gene-knockout and enzymological studies have defined IKKß as the main catalytic subunit of the IKK complex, possessing the bulk of the I
B
phosphorylation activity at serine residues 32 and 36 (Cao et al., 2001
; Li et al., 1999
; Li et al., 2000
; Wisniewski et al., 1999
). Surprisingly, the accumulation of phospho-I
B
at the PVM in T. gondii-infected cells is not caused by recruitment and activation of either IKKß or IKK
but by a unique parasite-derived activity designated TgIKK that exhibits a similar specificity for Ser32 and Ser36 as the mammalian IKK complex (Molestina and Sinai, 2005
).
We reasoned that the phosphorylation of IB
at the PVM by TgIKK might serve as the mediator of NF-
B activation promoting both nuclear translocation of NF-
B subunits and the accompanying upregulation of gene expression. In the present study, we address the roles of both the host IKK and parasite-derived TgIKK activities in the T. gondii-dependent activation of NF-
B target genes. Our results reveal a complex regulatory pattern of NF-
B activation that is crucially dependent on the integrity of the host IKK complex early in infection but requires TgIKK activity to sustain increased levels of gene expression. This response appears to be tailored by the parasite to attain selective modulation of host genes, which include crucial cytokines and pro-survival factors.
![]() |
Materials and Methods |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Immunofluorescence
Confluent WT, IKK/, IKKß/ and IKK
/ß/ MEFs were grown on sterile 12 mm glass coverslips placed in 24-well plates and infected at a multiplicity of infection (m.o.i.) of 5:1 with freshly passaged parasites for different periods of time. For short-term incubations (1-9 hours), the inoculum was centrifuged onto the cell monolayer at 800 g for 5 minutes at 4°C to synchronize invasion of MEFs. Procedures for immunofluorescence analysis (IFA) were performed as described previously (Molestina et al., 2003
). Primary antibodies used in these experiments were: anti-phospho-I
B
Ser32 antibody (Santa Cruz Biologicals, cat. no. sc8404), anti-p50 antibody (provided by N. Rice, National Cancer Institute, Bethesda, MD), anti-p65 antibody (Santa Cruz Biologicals, cat. no. sc-372), anti-GRA3 antibody (Bermudes et al., 1994
), and anti-SAG1 antibody (Argene). Species-specific Oregon-Green- or Texas-Red-conjugated secondary antibodies were purchased from Molecular Probes.
Immunoblot analysis
WT, IKK/, IKKß/ and IKK
/ß/ MEFs were seeded separately in 6-well plates at 2x105 cells/well and allowed to adhere overnight. Cells were infected with freshly passaged parasites at an m.o.i. of 5:1 for the time points indicated. For short-term incubations (1-9 hours), the inoculum was centrifuged onto the monolayer at 800 g for 5 minutes at 4°C to synchronize invasion of cells. Immunoblot analysis of I
B
phosphorylation (Ser32) in whole-cell lysates and p50/p65 translocation in nuclear extracts was performed as described previously (Molestina et al., 2003
). Equal amounts of protein (20 µg for I
B
phosphorylation and 10 µg for nuclear translocation assays) were resolved for each cell line by SDS-PAGE prior to immunoblotting, and signals were detected using a chemiluminescence-based system (Pierce). Identical exposures, based on the signal in wild-type cells, were used on all samples. Where indicated, densitometric analysis of protein bands was performed using the ImageJ software (http://rsb.info.nih.gov/ij/). Integrated densitometric values (IDV) of infected cells were corrected for IDVs of protein bands from uninfected cells (0 hour time point).
Electrophoretic mobility shift assay
Extraction of nuclear proteins from infected cells and binding reactions for electrophoretic mobility shift assays (EMSA) were performed as reported elsewhere (Molestina et al., 2000; Molestina et al., 2003
). Supershift assays were performed with antibodies to p50 and p65.
Real-time RT-PCR analysis
Primers and reagents for real-time RT-PCR were purchased from SuperArray. RNA was isolated from uninfected and infected WT, IKK/, IKKß/ and IKK
/ß/ cells after 0, 1, 3, 6, 9 and 24 hours of incubation using RNeasy Mini kits (Qiagen). Synthesis of cDNA was performed with 1 µg of total RNA in a 25 µl reaction mixture containing 1xRT reaction buffer, 1 µg random hexamers, 0.5 mM dNTPs, 1 U RNAse inhibitor, and 10 U MMLV reverse transcriptase at 37°C for 1 hour. Real-time PCR assays included the addition of HotStart Taq DNA polymerase in a 25 µl reaction mixture containing 1 µl cDNA, 10 mM Tris-Cl, 50 mM KCl, 2 mM MgCl2, 0.2 mM dNTPs, 1 µl gene-specific primer mix and 1x SYBR Green I solution (Molecular Probes). Reactions were run at 95°C for 15 minutes and 40 cycles of 95°C for 30 seconds, 55°C for 30 seconds and 72°C for 30 seconds. A LightCycler 2.0 Instrument (Roche Diagnostics) was used to detect and record fluorescence signals from each reaction. Threshold cycle (Ct) values were calculated for a particular gene at the different time points examined using the instrument software. Differences in the levels of gene expression over time were determined for each condition line by relative quantification using the Delta Delta Ct (
Ct) method as suggested by the manufacturer (SuperArray). The numerical data were subjected to analysis of variance followed by the Bonferroni post-test using the GraphPad Prism software. A P value of <0.05 was used to determine statistical significance.
NF-B gene arrays
Unless stated otherwise, all reagents used in gene array experiments including specific primer sets and hybridization membranes were purchased from SuperArray (mouse NFB array, cat. no. MM-016). Infection of WT, IKK
/, IKKß/ and IKK
/ß/ MEFs with T. gondii was performed at a m.o.i. of 5:1 for 24 hours. Total RNA from both uninfected and infected cells was isolated and 5 µg used as template for synthesis of [
-32P]dCTP-labeled (ICN) cDNA probes with an NF-
B array-specific primer set. Hybridization conditions of cDNA probes and calculation of IDVs from cDNA signals hybridized to each gene were performed as described (Molestina et al., 2003
). To determine fold differences in gene expression after infection, IDVs were normalized to actin since glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is known to be upregulated by T. gondii infection (Blader et al., 2001
). Intensity ratios between infected and uninfected cells were calculated for each gene using the GEArray Analyzer software (SuperArray).
![]() |
Results |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
|
We quantified the proportions of each group of vacuoles in infected WT and IKK-knockout cells as a measure of TgIKK activity at the PVM as a function of time of infection. As shown in Fig. 1G-J, the early phase of infection (1-3 hours) was characterized predominantly by group I vacuoles, indicating little-to-no PVM-associated phospho-IB
following invasion. The intermediate phase of infection, which correlates with the completion of the first round of parasite replication (6-9 hours), showed a noticeable increase in localized phospho-I
B
coincident with a rise in group II and III vacuoles. The highest levels of phospho-I
B
at the PVM were seen at the late phase of infection (18-24 hours), featuring a high proportion of group III vacuoles (Fig. 1G-J). A small proportion of vacuoles with a low level of staining were still observed at this late phase as depicted by the yellow arrows in Fig. 1E,F. The increase in phospho-I
B
localization observed over time was not restricted to WT MEFs since essentially identical kinetic profiles were observed in IKK
/, IKKß/ and IKK
/ß/ MEFs (Fig. 1G-J). Therefore, the appearance of phospo-I
B
at the PVM does not correlate with parasite invasion but rather by parasite growth.
An intact IKK complex is crucial for activation of NF-B early in infection
The lack of TgIKK activity early in infection suggests that the host IKK might play an instrumental role in activation of NF-B in the early phase of infection. To determine whether the kinetics of I
B
phosphorylation were affected by a loss of IKK function at the population level, analysis of phospho-I
B
was performed by immunoblotting. As shown in Fig. 2A, an increase in I
B
phosphorylation was apparent by 1 hour post-infection (p.i.) in WT MEFs at a phase where TgIKK activity was negligible (Fig. 1G). Interestingly, levels of I
B
phosphorylation were maximal at 9 hours p.i. (Fig. 2A) concurrent with an increase in TgIKK activity at the PVM (Fig. 1G). A slight decrease in I
B
phosphorylation was consistently observed at 24 hours p.i.; however, this response was still higher than uninfected cells, suggesting persistent activation (Fig. 2A, compare the time points for 0 and 24 hours). The kinetic profile of I
B
phosphorylation in IKK
/ MEFs was essentially identical to WT MEFs (Fig. 2B). As reported previously (Molestina et al., 2003
), a marked degradation of I
B
was not observed throughout infection despite elevated levels of phosphorylation (Fig. 2A,B).
|
Roles of host IKK and TgIKK in the T. gondii-mediated nuclear translocation of NF-B
Phosphorylation of IB
is the crucial event promoting nuclear translocation of NF-
B. In the absence of TgIKK activity at the PVM early in infection, we reasoned that translocation of NF-
B at this stage would indicate involvement of the host IKK complex. Likewise, a late response would correlate with an increase in TgIKK activity at the PVM at a time of host IKK activity dampening (Hoffman et al., 2002; Nelson et al., 2004
).
The kinetics of p50 and p65 translocation in WT MEFs (Fig. 3A,B and C,D, respectively) indicated a profile that paralleled the phosphorylation of IB
(Fig. 2A). The profile presents as a distinctly biphasic event with a second sustained `wave' of NF-
B translocation correlating with the appearance of TgIKK at the PVM. Surprisingly, despite near-WT phosphorylation levels of phospho-I
B
, infection of IKK
/ cells exhibited a profound defect in the nuclear translocation of both p50 (Fig. 3A,B) and p65 (Fig. 3C,D), particularly early in the infection. However, a small but reproducible increase in p50 and p65 levels in the nucleus was observed by 9 to 24 hours p.i., consistent with the appearance and accumulation of TgIKK at the PVM. Nuclear translocation of p50 and p65 was similarly deficient at early stages of infection in the IKKß/ and IKK
/ß/ backgrounds but the IKK
/ß/ cells failed to show a steady increase even at 18-24 hours p.i.
|
|
|
T. gondii infection induces an IKK-subunit-dependent modulation of gene expression
The results presented above show that a disruption in any of the catalytic components of the host IKK complex results in a defective NF-B translocation response to infection even in the presence of optimal levels of I
B
phosphorylation as seen with IKK
/ MEFs. We reasoned that the modulation of gene expression in the host cell might be controlled in a hierarchical fashion by sequential contributions from endogenous IKK and parasite-derived TgIKK activities. The steady state levels of gene expression were initially examined at 24 hours p.i. to provide an overview of the NF-
B-dependent transcriptional response induced by T. gondii.
DNA hybridization arrays focused on the NF-B pathway were probed with radiolabeled cDNA from uninfected and T. gondii-infected cells. Arrays consisted of 65 genes implicated in the regulation of the NF-
B pathway and 31 NF-
B-responsive genes. The results in Fig. 6 represent fold changes in gene expression between infected and uninfected cells and depict the means of three experiments. The complete data sets with means ± s.d. representing changes in expression for each gene in response to infection are shown in Table S1 (supplementary material).
|
The overall transcriptional response in IKK mutant cells was considerably reduced among key regulators of the NF-B pathway and NF-
B target genes (Fig. 6). Although 50% of all genes in the array were upregulated by at least threefold in WT MEFs, only 12% in IKK
/, 6% in IKKß/ and 4% in IKK
/ß/ MEFs showed a similar response. Interestingly, nearly 30% of all genes in the array were downregulated by threefold or more in IKK
/ß/ cells as a result of infection. These included genes that were markedly upregulated in WT cells (Fig. 6). Such a downregulatory trend was not as dramatic among cells lacking individual IKK subunits. In fact, a tendency for subsets of genes showing different levels of expression dependent on either IKK
or IKKß was observed. Thus, the induction of TRAF1, TRAF2, RelA and the ICAMs was affected to a higher extent by the lack of IKK
compared with IKKß. Likewise, levels of TLR1, TLR2, I
Bß, RelB and TNF-
expression showed a dependency on IKKß (Fig. 6). An exception to the broad suppression of gene expression in IKK
/ß/ MEFs was the induction of early growth response 1 (EGR1) and interferon
(IFN-
). Levels of expression of these genes were at least fourfold greater in all cell lines, reflecting the capability of T. gondii to trigger diverse pathways of activation independent of IKK (Blader et al., 2001
).
Altogether, the divergence of gene expression profiles affected by the lack of one or two IKK subunits during infection supports an essential role for the integrity of an active complex in the host cell. In addition, these results suggest that the integration of signals deriving from both an active IKK signalosome early in infection and TgIKK late in infection affects the magnitude of the NF-B-dependent transcriptional response.
Temporal analysis of gene expression reveals a biphasic response during infection
The array experiments revealed an induction of several early response genes, including pro-inflammatory cytokines whose upregulation was affected by the IKK background of the infected host cell. As representative genes from this group, we examined the kinetics of IL-6 and GRO1 expression by real-time quantitative RT-PCR. Infection of WT cells resulted in fluctuating patterns of induction of these genes (Fig. 7A,B, respectively). These profiles suggest distinct stages of activation that can be condensed into two main phases determined by an early host IKK response (1-3 hours) and a subsequent TgIKK-dependent component later in infection (9-24 hours). This biphasic response in gene expression was not clearly defined in the IKK mutant cell lines owing to the marked abrogation in IL-6 and GRO1 expression compared with WT (Fig. 7A,B, respectively). More importantly, in the absence of the early IKK-dependent response, the appearance of TgIKK at the PVM at intermediate and late stages of infection (Fig. 1) is unable to promote high levels of activation of these genes.
|
![]() |
Discussion |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
The timing of phospho-IB
localization at the PVM by 6-9 hours p.i. correlates with a phase of host IKK dampening (Hoffman et al., 2002; Nelson et al., 2004
). This suggests a level of temporal regulation that ensures sustained and regulated activation of the NF-
B pathway even in the presence of damped host IKK activity. In the context of infection, such a property might be required for effective manipulation of the cellular responses linked to immune and pro-survival functions by fine tuning the intensity of host gene expression.
The existence of the parasite-derived TgIKK activity suggested that host IKK was expendable in the sustained activation of NF-B in infected cells. In an earlier study (Molestina et al., 2005
), we reported that the ability of T. gondii to establish high levels of phospho-I
B
by immunoblot analysis correlated with the reported catalytic activities of IKK
and IKKß (Cao et al., 2001
; Li et al., 1999
). This requirement is further reinforced in the present study upon examination of the kinetics of I
B
phosphorylation in response to infection. Accordingly, we find rapid and sustained phosphorylation of I
B
in WT and IKK
/ cells (Fig. 2A,B). The initial activation is largely absent in IKKß/ and IKK
/ß/ cells, implicating IKKß as the main contributor to the early response (Fig. 2C,D). More importantly, in the absence of IKKß, the higher phosphorylation levels of I
B
are not attained later in infection, despite the presence of TgIKK activity at the PVM.
The hierarchy of host IKK and TgIKK activities in phosphorylating IB
is further reinforced in the temporal analyses of NF-
B translocation and subsequent gene expression. The pattern of p50 and p65 nuclear translocation in the early phase of infection (1-3 hours) of WT cells exhibits the classic damped oscillation profile of the NF-
B signaling module (Hoffman, 2002; Nelson, 2004), which is consistent with the activation of the host IKK signalosome. Translocation of p50 and p65 occurs rapidly in infected WT MEFs and the signal becomes dampened at 6 hours p.i. (Fig. 3). Of note, this `dampened signal' still represents a net translocation of NF-
B relative to the uninfected control (0 hour time point). The 6 hours time point corresponds to a phase when elevated levels of phospho-I
B
begin to accumulate at the PVM as a consequence of TgIKK activity (Fig. 1). Coincident with this increasing signal, a steady increase in NF-
B translocation is observed as infection progresses to reach a sustained level of activation. It is worth mentioning that this sustained response does not represent complete nuclear translocation of p50 and p65 (Fig. 4). Thus, the fine control of NF-
B translocation in T. gondii-infected cells represents a sophisticated level of subversion that is beyond a simple `on and off' switch.
The pattern of NF-B translocation in IKK-knockout cells revealed interesting results. Although cells lacking IKK
/ exhibited no major defect in the phosphorylation of I
B
early in infection, they presented a marked failure to promote both p50 and p65 nuclear translocation at this stage (Fig. 3). This absence of translocation was more expected in cells devoid of IKKß (i.e. IKKß/ and IKK
/ß/), which had major defects in I
B
phosphorylation at all time points. Thus, we propose that the optimal activity of TgIKK is crucially dependent on the establishment of an `activation threshold' by the host IKK. In such a scenario, the host IKK serves as the engine driving the initial activation of the pathway, whereas TgIKK activity is required to sustain and modulate the response. Importantly, however, TgIKK alone lacks the capacity to promote robust NF-
B activation, as noted by the absence of nuclear localization of p50 and p65 in IKK
/ß/ cells (Fig. 4).
The kinetics and magnitude of NF-B translocation correlated with the activation profile of IL-6 and GRO1. Interestingly, the regulation of these genes displayed fluctuating patterns of expression consistent with waves of NF-
B activation and de-activation. These oscillating patterns of activation fall into two phases characterized by a rapid increase early in infection and a phase of maximal expression coincident with elevated TgIKK activity at the PVM. A functional IKKß subunit in IKK
/ cells was not sufficient to induce optimal levels of IL-6 and GRO1 expression (Fig. 7), which might be partially explained by the defect in NF-
B translocation observed early in infection (Fig. 3). In addition, the deficient induction of gene expression in IKK
/ cells might be attributed to the role of IKK
in phosphorylation of histone H3 and p65, which affects expression of NF-
B target genes (Anest et al., 2003
; Yamamoto et al., 2003
; Lawrence et al., 2005
). Of note, the profound defects in parasite-dependent gene activation owing to IKK
or IKKß deficiency appear to be selective for early response genes as the expression of the anti-apoptotic gene IAP2 was affected to a much lesser extent (Fig. 7) despite being NF-
B regulated (Wang et al., 1998
). These observations suggest that the activation of subsets of anti-apoptotic genes entails the participation of additional signaling networks during infection.
In addition to supporting the essential requirement for IKK and IKKß in promoting a robust NF-
B transcriptional response by T. gondii infection, our gene array studies revealed the necessity for an intact signalosome in stimulating optimal expression of upstream regulators of the pathway (Fig. 6). Of note, clusters of genes displayed tendencies towards IKK
or IKKß activation for optimal expression. It is well recognized that distinct stimuli preferentially activate the IKKß-dependent `canonical pathway' or IKK
-dependent `alternative pathway' of NF-
B, resulting in the enhanced expression of specific genes (Bonizzi and Karin, 2004
). Nuclear translocation of p50/p65 heterodimers is dependent on the canonical pathway, whereas activation of the alternative pathway results in p52/RelB translocation. A previous study by our group determined the presence of p52/RelB in addition to p50/p65 heterodimers in nuclear extracts of T. gondii-infected cells by EMSA (Molestina et al., 2003
), supporting the activation of both arms of the NF-
B response by the parasite. As opposed to the early translocation observed with p50/p65, recent studies on the kinetics of p52/RelB translocation show a delayed profile dependent on IKK
as predicted (data not shown). The temporal differences in the activation of the canonical and alternative pathways suggest an additional level of T. gondii-derived regulation of NF-
B that might therefore govern parasite virulence.
Population genetic studies indicate that the three major lineages of T. gondii (Type I, Type II and Type III) appear to differ in their capacity to promote NF-B nuclear translocation and gene expression (Robben et al., 2004
; Saeij et al., 2005
). Our studies have focused on the hypervirulent Type I RH strain of the parasite. We and others find that infection with the RH strain promotes robust phosphorylation of I
B
(Fig. 2) (Molestina et al., 2003
; Butcher et al., 2001
; Shapira et al., 2005
) that is accompanied by the detection of NF-
B in the nucleus by IFA (Fig. 4), immunoblot (Fig. 3) and EMSA (Fig. 5) (Molestina et al., 2003
; Kim et al., 2001
). More importantly, as confirmed in this study, infection is associated with the increased expression of NF-
B-regulated genes both at early and late time points (Fig. 6) (Blader et al., 2001
; Brenier-Pinchart et al., 2000
; Denney et al., 1999
; Molestina et al., 2003
). Finally, the activation of a subset of these genes is not observed in (RelA) p65/ cells, reinforcing the role of NF-
B in the induction of gene expression (Molestina et al., 2003
). Despite this body of evidence, the issue of the activation of NF-
B translocation and gene expression in response to infection with RH strain parasites has recently become an area of controversy (Sinai et al., 2004
; Shapira et al., 2004
).
In contrast to our observations, the alternative view posits that whereas infection with RH-strain parasites promotes the phosphorylation of IB
, nuclear translocation of NF-
B subunits is absent (Butcher et al., 2001
; Shapira et al., 2002
; Shapira et al., 2005
). In a recent study, Shapira et al. define the absence of NF-
B translocation, both in early and late infection, relative to the level of NF-
B translocation observed following stimulation with arguably the most potent activator, TNF-
(Shapira et al., 2005
). They conclude, on the basis of this comparison, that there is no translocation of NF-
B in response to infection when in fact nuclear labeling of p65 is apparent, albeit at a much lesser extent relative to TNF-
, in infected cells (Shapira et al., 2005
).
The proposed blockade of NF-B subunit translocation, particularly early in infection, is also instituted in macrophages, epithelial cells and fibroblasts, including murine fibroblasts used in our studies (Butcher et al., 2001
; Shapira et al., 2002
; Shapira et al., 2005
). Notably, the lack of a complete nuclear re-distribution of NF-
B subunits mirrors our experience, showing that T. gondii infection results in only a fraction of NF-
B accumulating in the nuclei of infected cells (Fig. 4) (Molestina et al., 2003
). Regardless, however, the more central issue is whether these levels of nuclear NF-
B are sufficient to drive the parasite-directed activation of gene expression. The data sets from our group (Figs 6, 7) (Molestina et al., 2003
) and those of others (Blader et al., 2001
; Brenier-Pinchart et al., 2000
; Denney et al., 1999
; Kim et al., 2001
) indicate that Type I RH strain parasites drive and differentially regulate NF-
B-dependent gene expression. By contrast, the claim in support of a failure by T. gondii to activate NF-
B-dependent gene expression is not based on the analysis of endogenous genes but the use of a NF-
BGFP reporter system (Shapira et al., 2005
). At the very least, the differences in the results and interpretation point to the need for a thorough and detailed dissection of both the parasite and host determinants in this complex interaction.
The use of MEFs with defined genetic defects in the IKK complex has allowed us to differentiate between host and parasite activities affecting the regulation of the NF-B response. The molecular events involved in this process are multi-factorial, requiring the orchestrated participation of both host IKK and parasite TgIKK activities to modulate the strength and duration of the NF-
B-dependent transcriptional response. We propose a model where the initial stages of infection trigger activation of the host IKK complex, resulting in a primary wave of NF-
B translocation and induction of gene expression (Fig. 8A). Upstream mediators of this early phase probably involve components of the TLR pathway (Mason et al., 2004b
; Mun et al., 2003
; Robben et al., 2004
; Scanga et al., 2002
). As infection progresses, an increase in TgIKK activity at the PVM triggers phosphorylation of a subset of I
B
molecules and a secondary wave of NF-
B activation (Fig. 8A, intermediate phase). The extent of this response is limited in the absence of a functional IKK complex early in infection. The potential role of an amplification loop through an autocrine mechanism (i.e. IL-1, TNF-
) such as that described for the related apicomplexan Theileria parva (Dobbelaere and Rottenberg, 2003
), cannot be excluded to maintain a persistent state of NF-
B activation at late stages of infection (Fig. 8A, late phase).
|
The extent of NF-B-dependent gene expression affected by the integration of signals deriving from the host IKK signalosome and TgIKK is hypothetically represented in Fig. 8B. The regulation of specific genes displays oscillatory patterns early in infection (Fig. 8B, blue line, early phase), plausibly in a similar fashion as reported with the transient phase of NF-
B activation by pro-inflammatory cytokines (Hoffmann et al., 2002
; Schmidt et al., 2003
). Following increased TgIKK activity at the PVM (Fig. 8B, green dashed line), an activation of the response during mid-infection results in sustained levels of NF-
B-dependent gene expression (Fig. 8B, blue line, intermediate-to-late phases). An impaired IKK complex in the host cell abrogates this response significantly owing to a failure to attain an `activation threshold' (dashed line) required by TgIKK for the maintenance of persistent yet regulated levels of NF-
B activation (Fig. 8B, orange line).
Different pathogens have evolved a variety of strategies to evade or exploit the NF-B pathway to achieve successful co-existence with the host (Tato and Hunter, 2002
). Given the bimodal characteristics of NF-
B signaling and selective gene activation by classical activators of the pathway (Hoffmann et al., 2002
; Schmidt et al., 2003
), our study emphasizes the relevance of analyzing the regulation of host gene expression in a temporal fashion in response to infection. By analogy to T. gondii, the temporal profiles of selective NF-
B target genes modulated by infection with other pathogens might display a hierarchical regulation between host- and microbial-derived components.
![]() |
Acknowledgments |
---|
![]() |
Footnotes |
---|
![]() |
References |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Anest, V., Hanson, J. L., Cogswell, P. C., Steinbrecher, K. A., Strahl, B. D. and Baldwin, A. S. (2003). A nucleosomal function for IkappaB kinase-alpha in NF-kappaB-dependent gene expression. Nature 423, 659-663.[CrossRef][Medline]
Bermudes, D., Dubremetz, J. F. and Joiner, K. A. (1994). Cloning of a cDNA encoding the dense granule protein GRA3 from Toxoplasma gondii. Mol. Biochem. Parasitol. 68, 247-257.[CrossRef][Medline]
Blader, I. J., Manger, I. D. and Boothroyd, J. C. (2001). Microarray analysis reveals previously unknown changes in Toxoplasma gondii-infected human cells. J. Biol. Chem. 276, 24223-24231.
Bonizzi, G. and Karin, M. (2004). The two NF-kappaB activation pathways and their role in innate and adaptive immunity. Trends Immunol. 25, 280-288.[CrossRef][Medline]
Brenier-Pinchart, M. P., Pelloux, H., Simon, J., Ricard, J., Bosson, J. L. and Ambroise-Thomas, P. (2000). Toxoplasma gondii induces the secretion of monocyte chemotactic protein-1 in human fibroblasts, in vitro. Mol. Cell. Biochem. 209, 79-87.[CrossRef][Medline]
Butcher, B. A., Kim, L., Johnson, P. F. and Denkers, E. Y. (2001). Toxoplasma gondii tachyzoites inhibit proinflammatory cytokine induction in infected macrophages by preventing nuclear translocation of the transcription factor NF-kappa B. J. Immunol. 167, 2193-2201.
Caamano, J., Alexander, J., Craig, L., Bravo, R. and Hunter, C. A. (1999). The NF-kappa B family member RelB is required for innate and adaptive immunity to Toxoplasma gondii. J. Immunol. 163, 4453-4461.
Caamano, J., Tato, C., Cai, G., Villegas, E. N., Speirs, K., Craig, L., Alexander, J. and Hunter, C. A. (2000). Identification of a role for NF-kappa B2 in the regulation of apoptosis and in maintenance of T cell-mediated immunity to Toxoplasma gondii. J. Immunol. 165, 5720-5728.
Cao, Y., Bonizzi, G., Seagroves, T. N., Greten, F. R., Johnson, R., Schmidt, E. V. and Karin, M. (2001). IKKalpha provides an essential link between RANK signaling and cyclin D1 expression during mammary gland development. Cell 107, 763-775.[CrossRef][Medline]
Denkers, E. Y., Butcher, B. A., Del Rio, L. and Kim, L. (2004). Manipulation of mitogen-activated protein kinase/nuclear factor-kappaB-signaling cascades during intracellular Toxoplasma gondii infection. Immunol. Rev. 201, 191-205.[CrossRef][Medline]
Denney, C. F., Eckmann, L. and Reed, S. L. (1999). Chemokine secretion of human cells in response to Toxoplasma gondii infection. Infect. Immun. 67, 1547-1552.
Dobbelaere, D. A. and Rottenberg, S. (2003). Theileria-induced leukocyte transformation. Curr. Opin. Microbiol. 6, 377-382.[CrossRef][Medline]
Donald, R. G., Carter, D., Ullman, B. and Roos, D. S. (1996). Insertional tagging, cloning, and expression of the Toxoplasma gondii hypoxanthine-xanthine-guanine phosphoribosyl transferase gene. Use as a selectable marker for stable transformation. J. Biol. Chem. 271, 14010-14019.
Ghosh, S. and Karin, M. (2002). Missing pieces in the NF-kappaB puzzle. Cell 109, S81-S96.[CrossRef][Medline]
Hayden, M. S. and Ghosh, S. (2004). Signaling to NF-kappaB. Genes Dev. 18, 2195-2224.
Hoffmann, A., Levchenko, A., Scott, M. L. and Baltimore, D. (2002). The IkappaB-NF-kappaB signaling module: temporal control and selective gene activation. Science 298, 1241-1245.
Karin, M. and Lin, A. (2002). NF-kappaB at the crossroads of life and death. Nat. Immunol. 3, 221-227.[CrossRef][Medline]
Kim, J. M., Oh, Y. K., Kim, Y. J., Cho, S. J., Ahn, M. H. and Cho, Y. J. (2001). Nuclear factor-kappa B plays a major role in the regulation of chemokine expression of HeLa cells in response to Toxoplasma gondii infection. Parasitol. Res. 87, 758-763.[CrossRef][Medline]
Lawrence, T., Bebien, M., Liu, G. Y., Nizet, V. and Karin, M. (2005). IKK limits macrophage NF-
B activation and contributes to the resolution of inflammation. Nature 434, 1138-1143.[CrossRef][Medline]
Li, Q., Van Antwerp, D., Mercurio, F., Lee, K. F. and Verma, I. M. (1999). Severe liver degeneration in mice lacking the IkappaB kinase 2 gene. Science 284, 321-325.
Li, Q., Estepa, G., Memet, S., Israel, A. and Verma, I. M. (2000). Complete lack of NF-kappaB activity in IKK1 and IKK2 double-deficient mice: additional defect in neurulation. Genes Dev. 14, 1729-1733.
Mason, N. J., Artis, D. and Hunter, C. A. (2004a). New lessons from old pathogens: what parasitic infections have taught us about the role of nuclear factor-kappaB in the regulation of immunity. Immunol. Rev. 201, 48-56.[CrossRef][Medline]
Mason, N. J., Fiore, J., Kobayashi, T., Masek, K. S., Choi, Y. and Hunter, C. A. (2004b). TRAF6-dependent mitogen-activated protein kinase activation differentially regulates the production of interleukin-12 by macrophages in response to Toxoplasma gondii. Infect. Immun. 72, 5662-5667.
Mercurio, F., Young, D. B. and Manning, A. M. (2000). Detection and purification of a multiprotein kinase complex from mammalian cells. IKK signalsome. Methods Mol. Biol. 99, 109-125.[Medline]
Molestina, R. E. and Sinai, A. P. (2005). Detection of a novel parasite kinase activity at the Toxoplasma gondii parasitophorous vacuole membrane capable of phosphorylating host IkappaBalpha. Cell. Microbiol. 7, 351-362.[Medline]
Molestina, R. E., Miller, R. D., Lentsch, A. B., Ramirez, J. A. and Summersgill, J. T. (2000). Requirement for NF-kappaB in transcriptional activation of monocyte chemotactic protein 1 by Chlamydia pneumoniae in human endothelial cells. Infect. Immun. 68, 4282-4288.
Molestina, R. E., Payne, T. M., Coppens, I. and Sinai, A. P. (2003). Activation of NF-kappaB by Toxoplasma gondii correlates with increased expression of antiapoptotic genes and localization of phosphorylated IkappaB to the parasitophorous vacuole membrane. J. Cell Sci. 116, 4359-4371.
Mun, H. S., Aosai, F., Norose, K., Chen, M., Piao, L. X., Takeuchi, O., Akira, S., Ishikura, H. and Yano, A. (2003). TLR2 as an essential molecule for protective immunity against Toxoplasma gondii infection. Int. Immunol. 15, 1081-1087.
Nelson, D. E., Ihekwaba, A. E., Elliott, M., Johnson, J. R., Gibney, C. A., Foreman, B. E., Nelson, G., See, V., Horton, C. A., Spiller, D. G. et al. (2004). Oscillations in NF-kappaB signaling control the dynamics of gene expression. Science 306, 704-708.
Payne, T. M., Molestina, R. E. and Sinai, A. P. (2003). Inhibition of caspase activation and a requirement for NF-kappaB function in the Toxoplasma gondii-mediated blockade of host apoptosis. J. Cell Sci. 116, 4345-4358.
Richmond, A. (2002). NF-kappa B, chemokine gene transcription and tumour growth. Nat. Rev. Immunol. 2, 664-674.[CrossRef][Medline]
Robben, P. M., Mordue, D. G., Truscott, S. M., Takeda, K., Akira, S. and Sibley, D. (2004). Production of IL-12 by macrophages infected with Toxoplasma gondii depends on the parasite genotype. J. Immunol. 172, 3686-3694.
Saeij, J. P. J., Boyle, J. P. and Boothroyd, J. C. (2005). Differences among the three major strains of Toxoplasma gondii and their specific interactions with the infected host. Trends Parasitol. 21, 476-481.[CrossRef][Medline]
Scanga, C. A., Aliberti, J., Jankovic, D., Tilloy, F., Bennouna, S., Denkers, E. Y., Medzhitov, R. and Sher, A. (2002). Cutting edge: MyD88 is required for resistance to Toxoplasma gondii infection and regulates parasite-induced IL-12 production by dendritic cells. J. Immunol. 168, 5997-6001.
Schmidt, C., Peng, B., Li, Z., Sclabas, G. M., Fujioka, S., Niu, J., Schmidt-Supprian, M., Evans, D. B., Abbruzzese, J. L. and Chiao, P. J. (2003). Mechanisms of proinflammatory cytokine-induced biphasic NF-kappaB activation. Mol. Cell 12, 1287-1300.[CrossRef][Medline]
Shapira, S., Speirs, K., Gerstein, A., Caamano, J. and Hunter, C. A. (2002). Suppression of NF-kappaB activation by infection with Toxoplasma gondii. J. Infect. Dis. 185, S66-S72.[CrossRef][Medline]
Shapira, S., Harb, O. S., Caamano, J. and Hunter, C. A. (2004). The NF-B signaling pathway: immune evasion and immunoregulation during toxoplasmosis. Int. J. Parasitol. 3, 393-400.
Shapira, S., Harb, O. S., Margarit, J., Matrajt, M., Han, J., Hoffmann, A., Freedman, B., May, M. J., Roos, D. S. and Hunter, C. A. (2005). Initiation and termination of NF-B signaling by the intracellular protozoan parasite Toxoplasma gondii. J. Cell Sci. 118, 3501-3508.
Sinai, A. P., Paul, S., Rabinovitch, M., Kaplan, G. and Joiner, K. A. (2000). Coinfection of fibroblasts with Coxiella burnetti and Toxoplasma gondii: to each their own. Microbes Infect. 2, 727-736.[CrossRef][Medline]
Sinai, A. P., Payne, T. M., Carmen, J. C., Hardi, L., Watson, S. J. and Molestina, R. E. (2004). Mechanisms underlying the manipulation of host apoptotic pathways by Toxoplasma gondii. Int. J. Parasitol. 34, 381-391.[CrossRef][Medline]
Tato, C. M. and Hunter, C. A. (2002). Host-pathogen interactions: subversion and utilization of the NF-kappa B pathway during infection. Infect. Immun. 70, 3311-3317.
Tenter, A. M., Heckeroth, A. R. and Weiss, L. M. (2000). Toxoplasma gondii: from animals to humans. Int. J. Parasitol. 30, 1217-1258.[CrossRef][Medline]
Wang, C. Y., Mayo, M. W., Korneluk, R. G., Goeddel, D. V. and Baldwin, A. S., Jr (1998). NF-kappaB antiapoptosis: induction of TRAF1 and TRAF2 and c-IAP1 and c-IAP2 to suppress caspase-8 activation. Science 281, 1680-1683.
Wisniewski, D., LoGrasso, P., Calaycay, J. and Marcy, A. (1999). Assay for IkappaB kinases using an in vivo biotinylated IkappaB protein substrate. Anal. Biochem. 274, 220-228.[CrossRef][Medline]
Yamamoto, Y., Verma, U. N., Prajapati, S., Kwak, Y. T. and Gaynor, R. B. (2003). Histone H3 phosphorylation by IKK-alpha is critical for cytokine-induced gene expression. Nature 423, 655-659.[CrossRef][Medline]