Institut für Biochemie I, Universität Regensburg, 93040 Regensburg, Germany
* Present address: Moscow state university, Biology Department, 119899 Moscow, Russia
Author for correspondence (e-mail: markus.fuhrmann{at}vkl.uni-regensburg.de)
Accepted July 16, 2001
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SUMMARY |
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Key words: RNAi, Chlamyopsin, Antisense
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INTRODUCTION |
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Short light flashes applied to an algal eye initiate a set of photoreceptor currents, which in turn trigger biochemical and electrical processes within the flagella (Sineshchekov and Govorunova, 1999). The resulting Ca2+ influx into the flagella causes a transient alteration of the flagellar beating. Small vesicles still containing the whole eyespot apparatus have been teared out from Volvox cells. Photocurrents were recorded immediately from these vesicles, whereas the residual cell was unresponsive to light. This was the most convincing evidence that the photoreceptor and the whole signal transduction system are located within the eyespot region (Braun and Hegemann, 1999). On the basis of a Poisson-statistical analysis of flash-induced behavioral responses, it has been suggested that individual directional changes occurring at low flash intensities and photophobic responses triggered by high intensity flashes are two independent processes that may be mediated by different rhodopsin species (Hegemann and Marwan, 1988). Reconstitution experiments with retinal analogs that cannot isomerize around certain double bonds supported this finding (Zacks et al., 1993).
Supplementation of white, retinal-deficient cells with 3H-retinal, or exchanging the endogenous retinal in purified eyespot membranes with 3H-retinal identified only one retinal binding protein, which has been purified and sequenced (Beckmann and Hegemann, 1991; Deininger et al., 1995). Owing to its sequence homology with invertebrate opsins it was named chlamyopsin (COP). Moreover, the exon-intron organization of the cop gene is related to that of animal opsin genes (Deininger et al., 2000). We propose that algal opsins are part of a photoreceptor complex that includes the pore protein constituting the high light-saturating conductance responsible for phobic responses (Holland et al., 1996). This would explain the extremely fast onset of the photoreceptor current after light flash (Sineshchekov et al., 1990; Holland et al., 1996). Antisera against COP impaired the light-regulated GTPase activity of eye-specific G-proteins in the chlorophycaea S. similis (Calenberg et al., 1998). This finding supported data showing that the COP from C. reinhardtii is functioning as a photoreceptor and suggested that a G-protein is involved in the transduction process. But, the identified opsin of C. reinhardtii as well as its relative V. carteri (VOP) (Ebnet et al., 1999) are highly charged and the overall sequences are hardly compatible with a seven-transmembrane-helix receptor, which precluded their full acceptance as a sensory photoreceptor. Although the identified algal opsins are the major proteins of the eye (Deininger et al., 1995; Fuhrmann et al., 1999), evidence arose that they may have functions other than serving as photoreceptors for behavioural responses. For example, volvoxopsin is abundant in eyes of somatic cells and is expressed in eyeless gonidia and in young embryos long before the differentiation between gonidia and somatic cells is completed (Ebnet et al., 1999).
A genetic analysis of the opsin function has been unsuccessful thus far because opsin mutants are not available and targeted gene disruption has yet to be established for any green alga. Molecular machinery for the induction of a homologous recombination process is present in C. reinhardtii and V. carteri, allowing efficient recombination between extrachromosomal elements (Sodeine and Kindle, 1993; Gumpel et al., 1994; Hallmann et al., 1997). However, the ratio between homologous and illegitimate recombination of genomic elements is only between 103 and 104 (Sizova et al., 2001). Apparently, integration of DNA occurs predominantly via non-homologous recombination at random loci of the Chlamydomonas genome (Debuchy et al., 1989).
To overcome the lack of methods to disrupt genes in green algae, we first tried an antisense-RNA approach in V. carteri (Ebnet et al., 1999). Briefly, isolated gonidia were transformed with a volvoxopsin gene (vop) containing exons 6-8 in reverse orientation. Biolistic transformation led to many insertions of this transgene into the genome. It was found that more than 10 gene copies were required to reduce the VOP content by a factor of 10. The reduction of the sense RNA was proportional to the antisense/sense ratio (as/s). The transformants with reduced opsin level showed a reduction of their phototactic sensitivity, whereas the phototactic rate at high light intensities was normal, exactly as expected for a reduction of the phototaxis photoreceptor. The swimming of these transformants looked normal under the microscope, but the rotation of the spheroid, which is also critical for the phototaxis, has not been tested. The transformants also showed less synchronized growth and development, which could not be explained. A more detailed analysis has been hampered by two factors. First, the Volvox transformants were not accessible to measurements of light-induced ionic currents. Second, the multi-copy transformants were unstable and the phenotype was lost or at least impaired within a few months (P.H., unpublished). Thus, complementary experiments in C. reinhardtii were deemed necessary.
Here we present the development of an efficient gene silencing method for green algae, which uses the combination of a genomic gene fragment in tandem with its cDNA counterpart in reverse orientation. The resulting transformants had reduced opsin levels, which allowed a detailed biophysical analysis of light behavioural responses.
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MATERIALS AND METHODS |
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Plasmid-construction
A summary of DNA constructs for the expression of simple nonsense or antisense RNA in C. reinhardtii is shown in Fig. 1. In plasmid pMF15 one additional nucleotide was introduced into exon 1 of a 5.5 kb BamHI-KpnI cop-gene fragment. Plasmid pMF38 was made from pSP109 (Stevens et al., 1995) by exchanging ble against a 0.7 kb cop-cDNA fragment (Deininger et al., 1995). In pMF43 the 0.7 kb cop cDNA was inserted into the XbaI site of pSP124 (Lumbreras et al., 1998). Plasmid pMF47 was made by placing a 0.5 kb genomic cop-PCR-product between the MscI and SacI sites of pCOPV2.5 in inverse orientation (Fuhrmann et al., 1999). The plasmid pSR25 was designed to produce a single s/as transcript in vivo (Fig. 2). It was generated by insertion of a 0.4kb SacI fragment of pKS-cop (Deininger et al., 1995) into the unique SacI site of pCOPV2.5.
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Transformation
Transformation was carried out according to the glass bead method (Kindle, 1990) with pArg7.8 (Debuchy et al., 1989) or pSI103 (Sizova et al., 2001) as selectable markers. Arginine prototroph transformants were selected on TAP-plates. AphVIII transformants were selected on TAP-plates supplemented with 10-20 mg/l paromomycin. The plasmids containing the antisense constructs contain no selectable marker, but integrate into the host genome in about 30-50% of recovered transformants.
Analysis of the opsin-content in wildtype and transformants
Single colonies of transformants were grown in 10 ml of selective medium to a density of 1-5x106/ml. Cells were harvested by centrifugation for 5 minutes at 2000 g and lysed by sonication in 10 mM MOPS, 1 mM EDTA, pH 6.8. Insoluble cell debris was removed by centrifugation for 5 minutes at 2000 g. Total protein concentration of supernatants (whole cell extract) was determined by BCA-reaction (Pierce). Supernatant containing 120 µg of protein was diluted to 20 µl with 10 mM MOPS, 1 mM EDTA, pH 6.8 and mixed with 20 µl of 2x sample buffer pH 8.8, boiled for 5 minutes, and centrifuged at 14,000 g for 1 minute. 15 µl of the supernatant was applied on a 10% Tricin-SDS/polyacrylamide gel. Protein was transferred to nitrocellulose (Amersham), probed with -COP antibodies (Deininger et al., 1995), and an alkaline phosphatase-labeled secondary antibody (Roche). Equal loading of each lane was confirmed by independent Coomassie stained gels.
RNA extraction and hybridization
RNA was extracted from 5x108 cells as previously described (Fuhrmann et al., 1999). Enrichment of poly-(A)+ RNA was performed on oligo(dT)-cellulose columns with standard procedures. RNAs were separated on denaturing formaldehyde-agarose gels and transferred to Hybond N+-nylon membranes (Amersham). DIG-labeled PCR-products of cDNA-fragments were used as gene probes and detected with anti-DIG-AP-conjugates and CDP-Star as substrate (Roche).
Physiological analysis of transformants
Light triggered ionic currents were measured with a cell suction technique described in detail previously (Holland et al., 1996). Motion analysis of single cells was performed as described (Hegemann and Bruck, 1989). Cells were grown in 50 ml TAP to a final density of about 106 cells/ml and differentiated into gametes by overnight incubation in nitrogen-free medium (NMM). Three hours before the experiment was started, the culture was diluted 1:25 in NMM and kept in dim light (<1 W m2 white light).
Photo-induced light scattering changes in cell suspensions were recorded as described (Uhl and Hegemann, 1990). The monitoring light (840 nm peak excitation), inefficient for either phototaxis or photosynthesis, was produced by a LED (6701SG, Hitachi). The actinic light was applied in the perpendicular direction to the monitoring light, its wavelength range was selected by a narrow-band interference filter (Schott KG, Wiesbaden, Germany; maximum transmission, 502 nm; 12 nm half band width) in combination with a heat protection filter. Light scattering changes were detected by an infrared-sensitive photodiode (860 nm, Hitachi) placed at a scattering angle of 16°, amplified by a custom-made preamplifier in series with a CyberAmp 320 signal conditioner (Axon Instruments, Union City, CA), and digitized by a TTL-1 Labmaster DMA board using pClamp 5.5 software (Axon Instruments) at 50 Hz. Cells were resuspended in NMM at a density of 1.7x106 cells ml1 and kept overnight on a rotary shaker under cool fluorescent lamps (at about 2 W m2). A fresh cell sample was used each time after changing the stimulus intensity to avoid light adaptation of the photosensory system during the experiment.
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RESULTS |
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Second, as the simplest approach for the production of asRNA, the complete cop cDNA (Deininger et al., 1995) was placed under control of the strong promoter and the 3'-end of the rbcS2 gene (plasmid pMF38) but no changes in cop expression were detected in any of the transfomants. Thus, we concluded that, despite the strong promoter used, the amount of antisense RNA in these cells was insufficient to lower cop-gene expression. Since the phleomycin binding protein has to be expressed in large amounts to provide resistance to zeocine, the ble gene with two introns (pSP124) (Lumbreras et al., 1998) was inserted between the rbcS2 promoter and the antisense cop cDNA (pMF43, Fig. 1) and used as the only selection marker. But still the COP reduction did not exceed 30%. Next, the strategy similar to the one used in V. carteri (Ebnet et al., 1999) also failed. An inverted fragment spanning exon 5 and 6, and including intron 5 linked to the forward directed exons 1 and 2 (pMF47) led to an opsin reduction of between 30 and 40%.
Interference of double-stranded RNA (dsRNA) with expression of specific genes was described first to occur in Caenorhabditis elegans (Fire et al., 1998). Comparable observations were made later in a large number of organisms, including Drosophila, protozoans such as Trypanosoma, and plants (Gura, 2000). Although the mechanism is still not understood in detail, RNA interference (RNAi) provides an extremely powerful tool to study gene function in many organisms. To test the potential of double stranded RNA (dsRNA) on the reduction of gene expression in C. reinhardtii, sense and antisense RNA of the cop coding region were generated in vitro and used in co-transformation experiments (Fig. 2a). 14 days after transformation the transformants were tested for their COP content. None of the 48 clones showed a reduction of more than 10%. Thus, either dsRNA was not functional in C. reinhardtii or the RNA was rapidly degraded before any RNA-inactivation mechanism became active. To achieve a dsRNA level that fluctuates in parallel with the endogenous cop RNA, a large fragment of the cop cDNA covering exon 1 to 5 was linked in inverse orientation to the corresponding genomic fragment under control of a 1.5 kb fragment of the endogenous cop promotor (pSR25 in Fig. 2a). The presence of the introns 1 to 4 prevented recombination of potential hairpin structures during cloning in E. coli. In analogy to the advantages of introns for expression of genes encoding dominant selectable markers (see above), it was anticipated that the presence of introns in pSR25 might assist in maintaining an expression level of the transgene similar to that of the authentic cop gene. We surmised that after excision of the introns, a self-complementary hairpin RNA (hpRNA) might be formed immediately. The resulting hpRNA would be a perfect inverted repeat without a linker between the sense and antisense region, as it was reported as the most efficient trigger for PTGS in plants (Smith et al., 2000).
About half of the C. reinhardtii transformants obtained by co-transformation with pSR25 and pArg 7.8 showed a significant reduction in their opsin content (Fig. 2b). Transformant T7 and T12 expressed less than 5 and 2% COP, respectively, in comparison with wild-type (wt) cells. The reduction of COP is most clearly seen from the protein pattern of eyespot preparations in Fig. 2c. Expression of hpRNA seems to be the most efficient method for a specific reduction of a selected protein in C. reinhardtii.
RNA analysis
To determine the influence of hpRNA on cop RNA levels in the recovered transformants, RNA-blot analysis was performed in the hpRNA transformants copd12 (copd=Cop deficient) with a full length cop-cDNA as a probe. As shown in Fig. 2d, in wild-type cells a single cop transcript of 1.7 kb is detectable. In copd12 the amount of this transcript was reduced to only threefold, which is less pronounced than we expected from the reduction of the protein (50-fold). In addition, a smaller transcript of a defined size around 1.3 kb was detected. This was surprising, because usually truncated RNAs are rapidly degraded by a specific set of endogenous nucleases under most conditions. To further examine the nature of the shorter RNA and to test whether the truncation is related to the RNAi-construct pSR25, a different probe covering only exon 1-3 of cop was used. In this second experiment more transformant mRNA than wt mRNA was applied in order to enable better visibility of the truncated fragment. The smaller probe only identified the full-length cop transcript. This finding points to the conclusion that the 1.3 kb RNA represents an RNA truncated at its 5'-end in a region covered by the hpRNA. The attempt to analyze the 5'-end of the transcript by 5'-RACE PCR was unsuccessful even after treatment with tobacco acid pyrophosphatase to remove a potential CAP structure.
Photocurrents and behavioral responses of COP-reduced transformants
Microscopic observations and measuring the average swimming speed of the copd-transformants with a motion analysis system (Holland et al., 1996) revealed a significant reduction of motility in many of them, but no correlation between the reduction of motility and the amount of the cop-gene product assayed by the western blot analysis was found.
Since fast swimming cells with COP levels below 10% of that of wild-type were not found, electrophysiological studies were carried out with two cw15 transformants that expressed 10 to 20% of the wild-type COP level and showed normal motility. The photoreceptor currents recorded in these transformants were identical to those of the wild-type cw15 cells, with respect to kinetics and light sensitivity (Fig. 3). Recording photocurrents from the cw15 strain by means of the suction pipette technique yields a lower success ratio, and the average amplitude of the recorded currents is smaller than that of the cw2 strain used in all earlier studies (Holland et al., 1996). This is probably due to somewhat rigid remains of the cell wall present in the cw15 strain, which interfere with formation of a high-resistance seal with the pipette.
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DISCUSSION |
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The photoreceptor for photomovement responses?
In RNAi transformants with reduced COP content, stimulus-response curves should unequivocally decide whether the opsin-related protein, COP, is the photoreceptor for the estimated function or not. This is because a reduction of the photoreceptor content must reduce the absolute light sensitivity of the system and shift the stimulus-response curve to higher light intensities. This is true under the assumption that most of the photoreceptor molecules in a wild-type cell are functional. However, if the majority of the 10,000 to 30,000 opsin molecules per cell were non-functional, neither the low threshold for photocurrents and phototaxis could be explained nor the large dynamic activity range extending over >4 decades of light intensity (Foster et al., 1984) (S. Ehlenbeck et al., unpublished). The unchanged phototaxis and photophobic responses and the identical stimulus-response curves in wild-type and COP-deficient transformants shown in this study should leave no doubt that the COP protein is neither the photoreceptor for photophobic responses nor for phototaxis. This claim should be regarded as proven at least for the tested time range of 1 minute and range of light stimulus intensities. This is in sharp contrast to earlier conclusions that were based on a set of biochemical and electrophysiological evidence summarized in the introduction above (Deininger et al., 1995; Holland et al., 1996; Calenberg et al., 1998).
Stimulus-response curves for phototaxis that shift to higher light intensities are difficult to interpret because phototaxis is intimately linked to a precise flagellar beating. The slightest motility change reduces the phototactic rate and possibly the phototactic threshold. This difficulty may have led to a misinterpretation of the phototaxis experiments in VOP-deficient transformats of Volvox carteri, where the stimulus-response curve was shifted tenfold to higher light intensities than those of wild-type spheroids (Ebnet et al., 1999).
The function of COP, and its relative VOP, as photoreceptors remained suspicious from the beginning of their identification. First, in contrast to their sequence homology to invertebrate rhodopsins, both sequences are highly charged and 7 seven transmembrane helices, typical for any rhodopsin found so far, could not be identified in any hydropathy plot (Deininger et al., 1995). Second, volvoxopsin was enriched in eyespots of somatic cells but eyeless and electrically unresponsive gonidia contain more VOP than the eye-containing somatic cells. Third, COP expressed in E. coli, S. pombe and P. pastoris did not form any chromophore that is red-shifted relative to free retinal (W. Deininger and P.H., unpublished). Fourth, the all-trans chromophore and its isomerization to 13-cis after light excitation, as studied in vivo on retinal supplemented white cells, favoured an archaean-type rhodopsin (Lawson et al., 1991; Hegemann et al., 1991; Takahashi et al., 1991), whereas COP and VOP are related to the animal opsin group. The conclusion that COP is not the photoreceptor for behavioural responses implies two further important consequences. First, the rhodopsin that triggers phobic responses and phototaxis still awaits identification, and second, the function of the major opsin-related retinal protein of the algal eye remains obscure.
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ACKNOWLEDGMENTS |
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