1 Department of Neurology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
2 Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
3 Department of Neurology, Columbia University, New York, NY, 10027-6902, USA
4 Department of Pathology, Columbia University, New York, NY, 10027-6902, USA
** Author for correspondence (e-mail: perezrg{at}pitt.edu)
Accepted 10 May 2005
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Summary |
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Key words: MN9D, PC12, dopamine, PP2A, Parkinson's disease
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Introduction |
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The regulation of TH activity in the short term depends on the phosphorylation of key seryl residues (Ser19, Ser31 and Ser40) in the amino-terminal regulatory domain (Campbell et al., 1986; Haycock, 1990
). Of these, phosphorylated Ser40 is a major contributor to DA synthesis in vivo (Ramsey et al., 1996
). In previous studies we saw a significant reduction in total TH phosphorylation in cells overexpressing
-Syn, however, at that time we were unable to identify which residue(s) of TH were affected (Perez et al., 2002
). More recently, antibodies specific for TH P-Ser40 became available allowing us to evaluate the impact of
-Syn on the phosphorylation state of that specific seryl residue. In preliminary studies we observed that cells with higher
-Syn levels had reduced Ser40 phosphorylation leading us to explore the mechanism by which
-Syn contributed to P-Ser40 dephosphorylation.
Reversible phosphorylation requires the activity of kinases and phosphatases. Although multiple kinases can phosphorylate TH on Ser40 in vitro, including cyclic GMP-dependent protein kinase (Rodriguez-Pascual et al., 1999; Roskoski et al., 1987
), CamKII (Campbell et al., 1986
; Yamauchi and Fujisawa, 1981
), PKC (Albert et al., 1984
; Vulliet et al., 1985
), MSK-1 (Toska et al., 2002
), MAPKAP kinases 1 and 2 (Sutherland et al., 1993
), it is the cAMP-dependent protein kinase PKA (Joh et al., 1978
) that predominantly phosphorylates TH Ser40 in vivo (Campbell et al., 1986
; Wu et al., 1992
). It is well documented that dephosphorylation of TH Ser40 occurs almost exclusively by the phosphatase PP2A (Berresheim and Kuhn, 1994
; Haavik et al., 1989
; Leal et al., 2002
), which reduces TH activity both in dopaminergic cells (Haavik et al., 1989
) and in rat striatum (Bevilaqua et al., 2003
). To test the means by which
-Syn reduces TH phosphorylation we explored the impact of
-Syn overexpression on PKA and PP2A in dopaminergic cells expressing different levels of
-Syn. Herein, we report our novel findings implicating
-Syn in the activation of PP2A.
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Materials and Methods |
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Generation of stably transfected inducible PC12 cell lines
-Syn was cloned into pcDNA3 as previously described (Stefanis et al., 2001
) followed by subcloning into the SKSP shuttle cloning vector using HindIII-XhoI. From this vector, SfiI and PmeI were used to subclone
-Syn into the ecdysone-inducible PBWN vector, downstream of the response element. These constructs form the basis of the `bomb system' for inducible transgene expression (Suhr et al., 1998
; Suhr et al., 2001
) and were generously provided by Fred Gage and Steve Suhr at the Salk Institute (La Jolla, CA, USA). PC12 cells were transfected with PBWN-
-Syn using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA), following the manufacturer's recommendations. One week after transfection, selection was begun in 500 µg/ml G418 and individual clones selected. Tebufenozide, which is a molt-inducing insecticide that mimics the action of ecdysone (Addison, 1996
), was used for induction of
-Syn expression. PC12 clone, Sm1 (plasmid) was only slightly inducible after 2.0 µM tebufenozide for 72 hours (Fig. 1A, right side, lane 2), making these cells excellent baseline controls for our highly inducible clone, Sm4 (
-Syn; Fig. 1A, right side, lane 3), which expressed high levels of
-Syn expression after tebufenozide treatment (100 nM-2.0 µM, 24-72 hours). Untransfected (UT) PC12 cells were utilized as additional baseline controls. The data presented on PC12 plasmid and
-Syn cells were obtained from induced cells.
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Co-immunoprecipitation
For co-immunoprecipitation all steps were carried out at 4°C. Adult rat striata were collected, weighed and homogenized in 5 volumes of ice-cold co-IP buffer, which contained 50 mM Tris pH 7.4, 100 mM NaCl, 5 mM EDTA, 0.3% Triton X-100, 10% glycerol plus aprotinin, leupeptin, 4-(2-aminoethyl) benzenesulfonyl fluoride (AEBSF), ß-glycerophosphate, and dithiothreitol to inhibit protease and phosphatase activities. Supernatants were collected after centrifugation at 17,000 g (Sorvall RC5B, Kendro Laboratory Products, Newtown, CT, USA). A control aliquot of each supernatant was separated and frozen prior to co-IP for total protein determinations. Samples were pre-cleared for 1 hour with 10 µl 1% BSA plus 25 µl each of protein A- and protein G-Sepharose beads (Zymed Laboratories, S. San Francisco, CA, USA). Immunoprecipitating antibodies (5 µg) were coupled to SiezeTM X beads according to the manufacturer's instructions (Pierce, Rockford, IL, USA). Equal aliquots of homogenate (5.0 mg/ml total protein) were incubated with antibodies or pre-absorbed antibodies. Immune complexes were eluted, separated on 10 or 15% Tris-glycine SDS-PAGE gels, transferred to nitrocellulose, reacted with the same primary antibodies described above, and visualized by chemiluminescence. MN9D and PC12 cell extracts were prepared using the same buffers and conditions described above except that antibodies were not coupled to SiezeTM X beads. As the -synuclein and PP2A antibodies are both of mouse origin, we saw IgG heavy (55 kDa) and light chains (25 kDa) in addition to antigens and some non-specific bands in some experiments (indicated by asterisks in the figure).
PKA assay
Lysates of MN9D cells were prepared, protein concentrations determined, and PKA activity measured using a kit according to the manufacturer's instructions (cat. no. 17-134, Upstate Biotechnology, Lake Placid, NY, USA). [32P]ATP from NEN-Perkin Elmer Life Sciences (Boston, MA, USA) was utilized for the assay and PKA activity was measured by scintillation counting with activity expressed as pmol phosphate incorporated/minute/µg protein.
PP2A assay
PP2A immunoprecipitation and activity were determined using a non-radioactive kit according to the manufacturer's instructions (cat. no. 17-127, Upstate Biotechnologies, Lake Placid, NY, USA). Lysates of MN9D and PC12 cells were prepared in 20 mM imidazole-HCl, 2 mM EDTA, 2 mM EGTA, pH 7.0 with aprotinin, benzamidine and AEBSF (all from Sigma-Aldrich, St Louis, MO, USA). Protein concentrations were determined using BCA and 0.5-1.0 mg protein was immunoprecipitated using an anti-PP2A catalytic subunit antibody (cat. no. 06-222, Upstate, Lake Placid, NY, USA) and protein A-Sepharose beads (Zymed Laboratories, South San Francisco, CA, USA). Equivalent immunoprecipitation of PP2A from all samples was confirmed by immunoblot. Immunoprecipitated PP2A was then tested for activity in a 10-minute reaction at 37°C, in which phosphopeptide (K-R-pT-I-R-R) dephosphorylation was assayed spectrophotometrically at 650 nm using Malachite Green. PP2A activity was determined for all samples relative to a phosphate standard curve with activity expressed as pmol incorporated phosphate/minute/µg protein.
Okadaic acid treatment
Okadaic acid binds to the catalytic subunit of PP2A and inhibits its activity. Although okadaic acid at high concentrations can inhibit PP1 as well as PP2A, it is well documented that dephosphorylation of TH Ser40 occurs almost exclusively by PP2A, not by PP1 activity (Berresheim and Kuhn, 1994; Dunkley et al., 2004
; Haavik et al., 1989
; Leal et al., 2002
). Furthermore, we treated cells with low to high dose okadaic acid (5 nM-1 µM) dissolved in DMSO (0.13 µM) (Garcia et al., 2002
; Haavik et al., 1989
) for 1 hour to assess the impact on PP2A inhibition in the presence of
-Syn overexpression and saw a similar effect. For baseline TH Ser40 phosphorylation, cells were treated with 0.13 µM DMSO for 1 hour without okadaic acid. Cell lysates were prepared, protein concentrations determined, and 20 µg of protein from each condition was separated by SDS-PAGE for immunoblotting and densitometry. Equal protein loading was also confirmed with Ponceau S staining of blots prior to antibody incubation. Phosphorylated Ser40 levels were normalized to total TH for all treatment conditions, providing an internal standard for each measure of P-Ser40 on TH. For
-Syn inducible PC12 cells, data were also normalized to relative
-Syn levels within treatment conditions.
Statistical analyses
Independent sample t-tests, linear regression, and one way ANOVA were performed using SPSS (SPSS Inc., Chicago IL, USA) or Instat (Graphpad, San Diego, CA, USA) software. Post hoc analyses were performed by the method of Tukey-Kramer for data significant at P<0.05 or better. Experiments were repeated a minimum of two to three times on separate occasions with some experiments being performed five or more times. Data are presented as the mean ± s.e.m. for all treatments.
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Results |
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To confirm that the effect on P-Ser40 was associated with -Syn levels in dopaminergic cells we generated additional clonal cell lines in which the expression of
-Syn was under the control of an inducible promoter. Using these induced PC12 cell lines we again measured the impact of
-Syn on P-Ser40. UT and plasmid PC12 cells had little
-Syn compared to the
-Syn-overexpressing PC12 cells, which had up to 20-fold more
-Syn than plasmid control cells when cells were induced for 72 hours (Fig. 1A, right side). Total TH was equivalent in all PC12 cells (Fig. 1B, right side) confirming that the increase in
-Syn did not alter TH expression in stably transfected PC12 cells. When we compared P-Ser40 levels between UT, plasmid and
-Syn PC12 cells we observed that while controls maintained equally high P-Ser40 levels, the
-Syn PC12 cells had reduced P-Ser40 levels (Fig. 1C, right side) similar to that observed in
-Syn-overexpressing MN9D cells (Fig. 1C, left side). When data from multiple experiments were plotted we again saw a large decrease in P-Ser40 levels only the
-Syn PC12 cells (Fig. 1D, right side) similar to the effect observed in MN9D cells (Fig. 1D, left side). To further probe the relationship between
-Syn overexpression and reduced TH Ser40 phosphorylation, we treated inducible
-Syn PC12 cells with different amounts of inducer. When analyzed by linear regression we identified a significant negative correlation between
-Syn and P-Ser40 (r=-0.93, n=15, P=0.0017). Taken together the data from both MN9D cells and inducible PC12 cells indicate that the phosphorylation of TH Ser40 is negatively regulated by
-Syn in dopaminergic cells. This observation led us to further explore how
-Syn contributed to TH dephosphorylation.
Overexpression of -Syn does not alter PKA protein levels or activity
Since PKA is the major kinase mediating Ser40 phosphorylation and because -Syn is so strongly implicated in enzymatic inhibition, e.g. ERK2, PLD2, TH (Iwata et al., 2001
; Jenco et al., 1998
; Perez et al., 2002
) we first hypothesized that the reduction in TH Ser40 phosphorylation was probably occurring by
-Syn inhibition of PKA. To test this we measured PKA protein levels in UT, plasmid transfected, and
-Syn-overexpressing MN9D cells and found them to be equivalent (data not shown). We then measured PKA activity and confirmed similar activity in all cells regardless of
-Syn levels (data not shown) revealing that the reduction in P-Ser40 in
-Syn-overexpressing cells was not due to PKA inhibition. We then turned out attention to PP2A, the phosphatase that dephosphorylates P-Ser40 on TH (Haavik et al., 1989
; Leal et al., 2002
).
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-Syn increases PP2A activity and binds to the PP2A catalytic domain
We measured PP2A activity from the MN9D and induced PC12 cells using a well-established immunoprecipitation and phosphatase activity protocol (Begum and Ragolia, 1996). As we found identical data for control UT and plasmid cells in all previous experiments, we utilized plasmid cells as baseline controls for this series of experiments. We first confirmed that equal amounts of PP2A had been immunoprecipitated with an antibody to the PP2A catalytic subunit (Fig. 3A). We then measured the activity of the immunoprecipitated PP2A and found a doubling of PP2A activity in
-Syn MN9D cells compared to plasmid transfected control MN9D cells (Fig. 3B, left side). We observed a similar increase in PP2A activity in
-Syn PC12 cells (Fig. 3B, right side).
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We then tested for an interaction between -Syn and PP2A in our cells by a co-immunoprecipitation assay.
-Syn was found to co-immunoprecipitate with PP2A from MN9D cells (not shown) and from inducible PC12 cells (Fig. 4A) confirming that soluble
-Syn interacts with PP2A as measured using the Syn-1 antibody for co-immunoprecipitation. We tested the association between
-Syn and PP2A in rat striatum (Fig. 4B), confirming that the proteins also interact when
-Syn and PP2A are expressed at endogenous levels.
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Inhibiting PP2A produces robust phosphorylation of TH Ser40 only in -Syn cells
To assess whether TH Ser40 phosphorylation would increase after PP2A activity was inhibited, we treated control and -Syn-overexpressing cells with the phosphatase inhibitor, okadaic acid, which inhibits PP2A with an IC50 of 10 nM. We prepared cell extracts from MN9D and PC12 cells and measured increases in P-Ser40 levels after okadaic acid treatment (5 nM-1.0 µM). Okadaic acid treatment resulted in significant increases in P-Ser40 levels in all cells relative to vehicle-treated parallel cultures. This increase was expected because dephosphorylated Ser40 residues are present on TH in all cells. When
-Syn MN9D cells were treated with low dose okadaic acid (10-100 nM) large significant increases in P-Ser40 levels were noted in
-Syn-overexpressing cells (Fig. 5A). A large increase in P-Ser40 was also observed in PC12
-Syn cells (Fig. 5B, P<0.01). When we quantified the relative increases in P-Ser40 after okadaic acid treatment for MN9D and PC12 cells, we found nearly identical effects in both dopaminergic cell lines (MN9D=2.2±0.72; PC12=2.84±0.57, P>0.05). We noted that the magnitude of the increase in P-Ser40 levels when PP2A activity was inhibited was greatest in
-Syn-overexpressing cells (Fig. 5A,B), which had low baseline levels of P-Ser40 (see Fig. 1C, lane 3) and in which PP2A activity was significantly elevated (Fig. 3B). Since it is well-documented that Ser40 dephosphorylation occurs almost exclusively by PP2A (Berresheim and Kuhn, 1994
; Dunkley et al., 2004
; Haavik et al., 1989
; Leal et al., 2002
) our findings indicate that (1) TH Ser40 is dephosphorylated by PP2A in dopaminergic cells, and (2) the Ser40 residue on TH remains accessible to PP2A even when
-Syn is overexpressed. All in all, these data provide the first indication that
-Syn contributes to PP2A activation, which has potential relevance to synucleinopathies.
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Discussion |
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We noted that increased -Syn expression produced a several-fold decrease in TH Ser40 phosphorylation in the MN9D and PC12 cells with elevated
-Syn levels. This was further assessed by measuring a dose effect of
-Syn on P-Ser40 reduction in the inducible PC12 cells in which we found a significant negative correlation between elevated
-Syn levels and a diminution of P-Ser40. Thus, using two independent dopaminergic cellular models we showed that only when
-Syn levels were elevated, whether by constitutive or inducible overexpression, did we see significant decreases in P-Ser40 levels. Because
-Syn reportedly inhibits multiple enzymatic activities (Iwata et al., 2001
; Jenco et al., 1998
; Perez et al., 2002
) we had anticipated identifying a role for
-Syn as an inhibitor of PKA, which proved not to be the case. We found no change in either PKA protein levels or PKA activity in cells overexpressing
-Syn, confirming that the decrease in Ser40 phosphorylation in
-Syn-overexpressing cells was not due to an effect of
-Syn on PKA kinase activity. We therefore turned our attention to PP2A, the enzyme that is responsible for P-Ser40 dephosphorylation on TH.
Haavik and colleagues originally showed that PP2A, the major serine/threonine phosphatase that regulates many signaling pathways in mammalian cells, is responsible for greater than 90% of the dephosphorylation of TH at Ser40 (Haavik et al., 1989). Incubation of adrenal chromaffin cells with okadaic acid in the aforementioned study, dramatically increased TH phosphorylation and TH activity, firmly establishing PP2A as a regulator of both Ser40 phosphorylation and TH activity in dopaminergic cells. More recently, using PP1- and PP2A-specific inhibitors to measure TH dephosphorylation in brain, Dunkley and colleagues (Leal et al., 2002
) reconfirmed the role of PP2A in both TH activity and phosphorylation state. In our studies we found that PP2A protein levels were not altered by
-Syn overexpression in dopaminergic cells, yet PP2A activity was significantly increased in
-Syn-overexpressing cells. To further verify that the effects on Ser40 were associated with changes in PP2A activity we treated cells with okadaic acid, and found that Ser40 phosphorylation became significantly elevated in cells overexpressing
-Syn that had low baseline P-Ser40 levels. These data implicate PP2A activation as the mediator of P-Ser40 dephosphorylation in our
-Syn cell lines. These findings in dopaminergic cells with increased
-Syn levels confirm that (1) PP2A is more active, and (2) a dramatic increase in P-Ser40 phosphorylation is achieved by blocking PP2A activity. Additionally, the data strongly suggest that
-Syn-mediated activation of PP2A may have reduced both TH activity and DA synthesis in our earlier studies (Perez et al., 2002
).
Co-localization of the various PP2A subunits is required for PP2A activation and is thought to occur by interactions of the various subunits with molecules such as chaperones. An active PP2A enzyme consists of a heterotrimer of the structural A subunit, a catalytic C subunit, and a regulatory B subunit (Dobrowsky et al., 1993). The A and C subunits are ubiquitously expressed (Mayer et al., 1991
) and form the catalytic complex (PP2A/C), which interacts with at least three different families of regulatory B subunits, as well as with certain tumor antigens (Mumby and Walter, 1993
). The regulatory B subunits of PP2A are known to be temporally expressed during development (Csortos et al., 1996
; Mayer et al., 1991
; McCright and Virshup, 1995
; Ruediger et al., 1991
) and neuron-specific isoforms have also been identified (Mayer et al., 1991
). The substrate specificity of PP2A appears to be determined by the regulatory B subunits (Cegielska et al., 1994
; Csortos et al., 1996
) and there is evidence that B subunits are associated with targeting the PP2A/C catalytic complexes to various intracellular sites such as microtubules (McCright et al., 1996
; Sontag et al., 1995
) and mitochondria (Ruvolo et al., 2002
) suggesting that PP2A complexes are actively trafficked by their associated interacting proteins, one of which may be
-Syn. We have discovered that
-Syn and PP2A interact with each other in soluble fractions of brain and dopaminergic cells as measured by co-immunoprecipitation. Membrane bound PP2A in brain is reportedly less active (Sim et al., 1998
), thus the interaction of PP2A with
-Syn within the cytosol may serve to stimulate PP2A activity. This interaction of
-Syn with PP2A may affect PP2A conformation or trafficking and subsequently contribute to its activation.
-Syn oxidative modification or aggregation occurs in neurodegenerative diseases such as Alzheimer's disease, a condition in which PP2A is also implicated (Trojanowski and Lee, 1995
; Zhao et al., 2003
). Based on our novel findings these are the first data to identify an association between
-Syn and PP2A that affects PP2A activity and may contribute to neuronal homeostasis, which if disrupted may be detrimental.
The impact of -Syn on TH P-Ser40 may involve other means of regulating the phosphorylation on this site. For example,
-Syn can directly interact with 14-3-3 (Ostrerova et al., 1999
) and with TH (Perez et al., 2002
). It is known that 14-3-3 can activate TH (Ichimura et al., 1988
) by 14-3-3 binding first to TH Ser19 then to TH Ser40 (Kleppe et al., 2001
). There is additional evidence that the binding of 14-3-3 to TH phosphorylated on Ser19 and Ser40 stabilizes its conformation and enhances TH activity (Bevilaqua et al., 2001
). An interaction of
-Syn with 14-3-3 may contribute to dissociation of 14-3-3 from TH to permit PP2A physical access to the Ser40 site with subsequent effects on TH phosphorylation and DA synthesis. Further studies are required to identify the precise manner by which
-Syn acts to stimulate PP2A activity. However, regardless of how it does so, we provide novel evidence that
-Syn interacts with and contributes to the activation of PP2A, a major brain phosphatase. Our findings also underscore the importance of further elucidating normal
-Syn function because (1) many substrates require PP2A for dephosphorylation, and (2)
-Syn is implicated in multiple synucleinopathies.
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Acknowledgments |
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Footnotes |
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Present Address: University of Pittsburgh, Department of Neurology, BST W905, Pittsburgh, PA 15213, USA
Present Address: University of Tennessee, Department of Physiology, 894 Union Avenue, Memphis, TN 38163, USA
¶ Present Address: Neurobiology Laboratory, Foundation of Biomedical Research of the Academy of Athens, Athens 11527, Greece
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