Fig. 10. Expression of laminin-binding integrins on transfected K562 cells increases
CD151 cell surface expression. Flow cytometric analysis of wild-type K562
cells endogenously expressing 5ß1 and transfected K562 also
expressing vß1, 3ß1, 6ß1, 6ß4,
7X1ß1 or 7X2ß1. Solid lines in the left panels
indicate staining with anti-integrin antibodies: anti-5 mAb Sam-1 (K562
cells), anti-v mAb 13C2 (K562-v), anti-3 mAb J143
(K562-3), anti-6 mAb GoH3 (K562-6), anti-ß4 mAb
439-9B (K562-6ß4) and anti-7 mAb CA25 (K562-7BX1 and
K562-7BX2). Staining of cells with anti-CD151 antibodies is shown in
the right panels. Solid lines represent staining of cells with mAb TS151R and
broken lines with mAb P48. A negative control (dotted line) with secondary
goat anti-mouse or rat IgG alone is shown in each panel. R represents the
ratio of the mean fluorescence intensity of cells stained with mAb P48 and
with secondary antibody alone. A three- to sixfold increase of CD151
expression is observed in transfected cells that ectopically express
lamininbinding integrins. The TS151R epitope is masked in all cells expressing
one of the laminin-binding integrins.