1 Institute of Cell Biology, Department of Biology, Swiss Federal Institute of Technology, ETH Hönggerberg, CH-8093 Zürich, Switzerland
2 Departments of Medical Genetics and Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
3 Institute of Molecular Biology Zürich, Winterthurerstr. 190, CH-8057 Zürich, Switzerland
4 Department of Molecular Medicine, Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, 82152 Martinsried, Germany
¶ Author for correspondence (e-mail: usuter{at}cell.biol.ethz.ch)
Accepted 22 March 2005
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Summary |
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Key words: Integrins, Neural progenitors, Neurospheres, CNS
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Introduction |
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Neural stem cells can be obtained from perinatal forebrain germinal zones and grown in vitro in the presence of EGF and/or FGF-2 as multipotent neurospheres with self-renewing capacity (Ciccolini and Svendsen, 1998; Tropepe et al., 1999
; Zhu et al., 1999b
). However, the apparent lack of specific stem cell markers makes it difficult to identify these cells both in vivo and in vitro, and the ability to form clonal multipotent neurospheres over several passages remains the best functional assay for the presence and quantification of putative NSCs within a cell population (Imura et al., 2003
). Neurospheres are intrinsically heterogeneous cellular entities almost entirely formed by a small fraction (1 to 5%) of slowly dividing neural stem cells and by their progeny, a population of fast-dividing nestin-positive progenitor cells (Campos et al., 2004
; Lobo et al., 2003
; Reynolds and Weiss, 1996
; Zhu et al., 1999b
). The total number of these progenitors determines the size of a neurosphere and, as a result, disparities in sphere size within different neurosphere populations may reflect alterations in the proliferation, survival and/or differentiation status of their neural progenitors.
Similar to other systems, the extracellular matrix constitutes an important source of instructive cues capable of regulating the behaviour of stem and progenitor cells in the developing CNS (Drago et al., 1991). The role of integrins in the recognition of such cues by cells of the CNS has been examined extensively (Clegg et al., 2003
; Milner and Campbell, 2002
). Integrins are a major group of cell-surface receptors for both extracellular matrix and cell-surface molecules (Montgomery et al., 1996
; Ruppert et al., 1995
). They are composed of two non-covalently associated transmembrane glycoproteins,
and ß, both of which participate in the binding of matrix proteins, and have been implicated in inside-outside signalling and in the coordination of the actin cytoskeleton and cellular response to growth factors (Hynes, 1992
). Integrins regulate several fundamental processes such as proliferation, migration, cell survival and differentiation in a variety of tissues (Danen and Sonnenberg, 2003
). They also play key instructive roles during the development of several embryonic regions (Adams and Watt, 1993
; Hynes, 1996
) including the developing CNS, where ß1-integrins influence the development of laminae and folia in the cerebral and cerebellar cortex (Georges-Labouesse et al., 1998
; Graus-Porta et al., 2001
)
Signalling via ß1 integrin also acts as a negative regulator of stem cell differentiation in various tissues, such as the skin (Brakebusch et al., 2000) and the prostate (Collins et al., 2001
). Prompted by these findings, we have recently shown that ß1-integrin signalling is likely to contribute to the maintenance of neural stem cells through the modulation of MAPK activity (Campos et al., 2004
). Here, we use control and ß1 integrin-deficient neurosphere cultures, to show that: (1) ß1-integrin signalling is not an absolute requirement for the maintenance of neural stem cell self-renewal; (2) ß1-integrin signalling, in cooperation with growth factor signalling, regulates the number of undifferentiated progenitor cells; (3) adhesion and migration of neurosphere-derived cells on ECM substrates is dependent upon ß1 integrin.
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Materials and Methods |
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Neurosphere cell culture
Neural precursors were obtained from dissociated postnatal day 1 forebrains of ß1-integrin lox/lox, lox/wt and lox/0 animals. Neurospheres were grown in DMEM/F12 supplemented with B27 (Invitrogen) using previously described methods (Reynolds et al., 1992; Reynolds and Weiss, 1996
; Svendsen et al., 1995
; Weiss et al., 1996
; Zhu et al., 1999b
) in the presence of 10 ng/ml EGF (human recombinant; Peprotech) and 20 ng/ml bFGF (human recombinant; Peprotech). Primary neurospheres were harvested after 7 days in culture, dissociated into single cells and plated at 6x104 cells/ml in the presence of an adenovirus expressing the Cre recombinase (Kalamarides et al., 2002
) used at 50 virus particles per cell. Fresh medium was added to the cultures 3 days after infection and the medium was changed 6 days after infection. 10 days after infection, primary infected neurospheres (passage 1) were harvested and either stained for X-gal or trypsinized for later passages. Neurospheres were dissociated in 0.25% trypsin (Invitrogen) for 10 minutes and subsequent mechanical dissociation in L15 Medium (Sigma) supplemented with 1 mg/ml trypsin inhibitor (Sigma) and 0.8 mg/ml DNase I (Roche).
FACS sorting
For FACS sorting, neurospheres were dissociated into single cells, washed twice with FACS-PBS (1 x PBS supplemented with 2% FCS), incubated with a FITC-conjugated hamster anti-ß1-integrin antibody (1:100 in FACS-PBS; BD Biosciences) for 30 minutes on ice and washed three times with FACS-PBS. The cells were kept on ice and the cell suspension was passed through a 40 µm cell strainer (Falcon) before sorting. FACS analysis was performed using a FACStar PLUS (Becton Dickinson) connected to a Macintosh running CellQuest Software. Cells were analysed for forward scatter, side scatter and ß1-integrin fluorescence using an Argon Laser (480 nm excitation, 520 nm emission). Dead cells and doublets were excluded by gating on forward and side scatter.
Short-term adhesion assays
Adhesion assays were performed as described previously (Milner et al., 2001). Briefly, small areas of bacteriological-grade plastic Petri dishes were coated with laminin-1, fibronectin or poly-D-lysine (PDL). After incubation at 37°C for 2 hours, substrates were washed twice with DMEM/F12. Cells derived from dissociated neurospheres were applied to the substrates in a 25 µl drop for between 5 and 60 minutes at 37°C. The adhesion assay was stopped by adding DMEM/F12 to the dishes, thereby washing off loosely attached cells. The attached cells were fixed, stained with Trypan Blue and washed twice with PBS. Adhesion was quantified by counting the attached, stained cells and results are expressed as a percentage of the number of cells adhering on PDL for each time point investigated. The experiments were done in duplicate and the results represent means±s.e. of three individual samples per genotype per substrate. Human laminin-1 (Chemicon) and fibronectin (Sigma) were diluted to the 10 µg/ml coating concentration in PBS; PDL (Sigma) was diluted to 5 µg/ml in PBS.
Immunocytochemistry
For whole mount X-gal staining, neurospheres were fixed in 0.2% glutaraldehyde in PBS for 30 minutes and stained in X-gal staining solution comprising 5 mM K3[Fe(CN)6], 5 mM K4[Fe(CN)6], 2 mM MgCl2 and 2 mg/ml X-gal (Calbiochem) in PBS. After staining, neurospheres were post-fixed in 2% formaldehyde/PBS at 4°C. For immunostaining on sections, neurospheres were gently centrifuged (300 rpm for 5 minutes), embedded in O.C.T. compound (Tissue Tek) and 12 µm cryosections prepared. Sections and dissociated single cells were fixed in 4% paraformaldehyde/PBS for 20 minutes, permeabilized in 0.3% Triton X-100 for 15 minutes at room temperature and incubated overnight with primary antibodies. As primary antibodies, rabbit anti-phospho-histone H3 (1:200; Upstate), rabbit anti-ß-galactosidase (1:200; Cappel), rabbit anti-ß1 integrin (1:200), monoclonal anti-nestin (1:200; BD Biosciences), rabbit anti-GFAP (1:200; DAKO), monoclonal anti-ß-III-tubulin (1:100; Sigma) and neurofilament (1:200; Sigma) were used. After incubation with the primary antibody, sections were washed three times for 5 minutes in PBS. Alexa 488-conjugated goat anti-rabbit (1:300; Molecular Probes) or Cy3-conjugated goat anti-mouse (1:300; Jackson Laboratories) were used as secondary antibodies. The TUNEL assay was carried out using indirect immunofluorescence to visualize nicked DNA according to the manufacturer's instructions (Apoptag Red, Intergen). Apoptotic cells were revealed by indirect immunofluorescence using an antibody recognizing endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 (1:250, Cell Signaling) resulting from cleavage adjacent to Asp175.
Pictures were acquired using a Zeiss conventional or confocal fluorescence microscope equipped with a Zeiss Axiocam CCD camera connected to a Macintosh computer. Images were processed using Open Lab 3.0 software (Improvision).
Self renewal assays and characterization of neurospheres
After adenovirus infection, neurospheres were passaged every 10 days and replated each time at 6x104 cells/ml (clonal density). To calculate the percentage of recombined spheres in mutant and control cultures, aliquots containing 150 to 250 neurospheres were obtained from individual cultures and stained for X-gal at different passages.
For measuring the size of the neurospheres, aliquots of the cultures were stained for X-gal and the diameter of the neurospheres was calculated from the cross-section of the neurospheres measured using Openlab 3.0 (Improvision) Software on a Macintosh. In total, the cross-section of 5001 neurospheres (1811 control and 3190 mutant spheres) from four independent experiments was measured. For determination of the cellular composition of the neurospheres, 8-day-old spheres were dissociated into single cells, plated onto PDL (Sigma)-coated chamber slides (Nunc) in DMEM/F12 supplemented with B27 for 3 hours, fixed and stained as described above. These experiments were done three times.
Maintenance and migration
Chamber slides (Nunc) were precoated with laminin-1, fibronectin or PDL. Intact, 9-day-old neurospheres were then plated onto the precoated chamber slides in DMEM/F12 supplemented with B27 with either no growth factors or with growth factors at limiting concentrations (3.3 ng/ml EGF, 2.0 ng/ml FGF, 10 ng/ml NGF). Cultures were left for 6 days to differentiate under the conditions given. The capacity of the individual clones to maintain progenitors and to generate neurons and glial cells was addressed by immunocytochemistry using antibodies against GFAP, nestin, ß-III-tubulin and neurofilament as described above. Migration was quantified by measuring the extent of migration of cells from the neurospheres. The average of the four largest distances per neurosphere was divided by the core diameter of the neurosphere, thereby compensating for the different sizes of the individual neurospheres. Maintenance and migration experiments were done three times. In each experiment, at least 15 neurospheres for each ECM substrate were analysed.
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Results |
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Loss of ß1-integrin surface expression disturbs adhesion of neurosphere cells to laminin and fibronectin substrates
Integrins are primarily extracellular matrix (ECM) adhesion receptors. To determine if the loss of surface ß1-integrin expression had a functional effect on cell adhesion, we compared the ability of recombined control (/wt) and mutant (
/0) neurosphere cell populations to bind to the ECM substrates laminin-1 (LM1) and fibronectin (FN) in short term adhesion assays. Although the complete repertoire of integrin expression in mouse neurospheres has not been described, it is likely that it will not significantly differ from that of rat neurospheres; the major ß1-containing integrins in rat neurospheres are the fibronectin receptor
5ß1, the laminin receptors
6Aß1 and, at lower levels,
6Bß1,
1ß1 and
3ß1, and also the vitronectin receptor
ß1, which shows some affinity for fibronectin substrates (Jacques et al., 1998
). Thus, by eliminating ß1 integrin from the surface of
/0 neurosphere cells, we predicted a reduction in their adhesion to both laminin-1 and fibronectin substrates. This was confirmed by our results, where we found that
/0 cells adhered significantly less to laminin-1 than
/wt control cells at all time points investigated (Fig. 2D), and to fibronectin substrates, at 30 and 60 minutes after plating (Fig. 2E). The effect on fibronectin was less pronounced than on laminin and may reflect the fact that other integrins capable of binding fibronectin, such as
ß5 and
ß8 may mediate binding to fibronectin, partially compensating for the lack of
5ß1.
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A synergy between growth factor receptor and integrin signalling has been described in various other systems (Aplin et al., 1999; Brooks et al., 1997
). Hence, a possibility remained that the lack of a ß1-integrin effect was masked by an increase in growth factor receptor signalling owing to the presumably high, non-physiological concentrations of EGF and FGF present in our assays. To address this point, we carried out neurosphere forming assays on passage ten of mutant and control populations in the absence of FGF and over a range of EGF concentrations. No significant difference was found between the percentage of mutant and control recombined spheres formed at 10, 5 and 1 ng/ml EGF (Fig. 3B). Note that the lowest EGF concentration investigated, 0.1 ng/ml, did not support either the formation of mutant or control neurospheres (Fig. 3B).
The percentage of small spheres is significantly increased in mutant ß1-null neurosphere cultures
Although the loss of ß1-integrin signalling does not significantly affect the capacity of mutant cells to form new neurospheres, it influences their size. In comparison with control (/wt) neurosphere populations, a significantly higher percentage of small (10-30 µm diameter) spheres was present in the mutant (
/0) neurosphere populations (Fig. 4A). Concomitantly, the percentage of larger (40-70 µm diameter) spheres present in mutant populations was significantly lower than that found in control populations (Fig. 4A). This effect was also found in neurosphere cultures grown in EGF only (data not shown) and persisted over later passages. As the nestin-positive, undifferentiated neural progenitor is the most abundant cell type present in a neurosphere (Jacques et al., 1998
; Lobo et al., 2003
; Reynolds and Weiss, 1996
; Zhu et al., 1999b
), it was likely that the size effect observed was caused by a decrease in the nestin-positive progenitors present in mutant neurospheres. To examine this hypothesis, we analysed the cellular composition of mutant and control neurospheres. Single cell suspensions, obtained by disaggregation of 8-day-old neurospheres from passage three or later, were plated on PDL-coated chamber slides for 8-12 hours, fixed and stained for nestin (a marker for undifferentiated neural progenitors), GFAP (an astrocyte marker) and ß-tubulin (class III; a neuronal marker). These experiments were done three times. We found that in the mutant cell populations the percentage of nestin-positive cells was significantly reduced (73.4±3.2% compared with 90.1±3.4% in the control; Fig. 4B) whereas both the percentages of GFAP-positive cells (18.2±2.6% compared with 8.9±1.5% in the control; Fig. 4B) and ß-III-tubulin-positive cells (8.3%±1.9% compared with 0.96±0.94% in the control; Fig. 4B) were significantly increased.
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As the loss of integrin signalling can induce programmed cell death (Gary et al., 2003; Gilmore et al., 2000
; Zhang et al., 1995
) and affect proliferation in different cell types (Hirsch et al., 2002
; Sastry et al., 1996
), we next investigated if the reduction in the number of mutant nestin-positive cells was caused by increased cell death, reduction in cell proliferation, or by both processes acting simultaneously.
Increased cell death and reduced proliferation limit the growth of mutant neurospheres
To identify proliferating undifferentiated neural progenitors we carried out double immunofluorescence staining on single cells using antibodies against nestin and phosphorylated histone H3, a cell division marker (Gurley et al., 1978; Hendzel et al., 1997
; Paulson and Taylor, 1982
). We found a significant reduction in the number of proliferating, nestin-positive mutant cells (3.9±1.5%; n=3; Fig. 4C) when compared with their control counterparts (10.6±1.9%; Fig. 4C). In both mutant and control cell populations virtually all proliferating cells were nestin positive (data not shown). Neural progenitors undergoing programmed cell death were identified on sister cell cultures, by combining the TUNEL assay with nestin immunostaining. The percentage of TUNEL-positive cells in mutant cell populations was significantly higher (14.8±1.6%; Fig. 4D) than that in control cell populations (2.2±0.6%, Fig. 4D). In contrast to control populations, where virtually no apoptotic nestin progenitor cells were present, in mutant cultures virtually all TUNEL-positive cells were also positive for nestin (15.7±3.5%; Fig. 4E). These results were further confirmed by indirect immunofluorescence using an antibody recognizing the large fragment (17/19 kDa) of activated caspase-3. Here, apoptosis in the mutant cultures (22.7±4.2%) was also significantly higher than apoptosis in control cultures (4.4±0.92%; n=3, P<0.005). Our results suggest that because of the lack of ß1-integrin signalling, more mutant nestin-positive cells die and also proliferate less than their control counterparts. These two factors acting in conjunction probably reduce the absolute number of mutant nestin-positive cells, thereby limiting the growth of the mutant neurospheres.
ß1-integrin signalling synergizes with growth factor signalling to regulate nestin progenitor cell survival
Although the mutant neurospheres displayed a significant phenotype in the high amounts of EGF (10 ng/ml) and FGF (20 ng/ml) present in our cultures, this did not exclude the possibility that the lack of ß1 integrin was partially compensated by such high concentrations of growth factors. In order to investigate this issue, we quantified the number of nestin-positive progenitors present in mutant and control neurospheres after 6 days of differentiation on laminin-1 or fibronectin-coated plates, in the absence or presence of growth factor concentrations (3.3 ng/ml EGF, 2.0 ng/ml FGF, 10 ng/ml NGF) that promote neurosphere cell survival but keep proliferation to a minimum (Ohtsuka et al., 2001). In the absence of exogenous growth factors, 96.8±3.2% of control ß1-integrin
/wt neurospheres plated on laminin-1 contained at least one nestin-positive cell per neurosphere explant. By contrast, in the mutant ß1-integrin
/0 cultures, this percentage dropped to 7.5±2.0% (Fig. 5A). However, if these mutant cultures were grown in the presence of growth factors, 85.1±8.6% of the neurospheres contained nestin-positive cells (Fig. 5A), indicating that growth factor signalling could almost completely override the lack of ß1 integrin. Similar results were found for neurospheres plated on fibronectin and on PDL (Fig. 5A). The results observed on PDL were probably owing to the fact that the ECM contains fibronectin and laminin, which are produced and deposited by the neurosphere itself (Campos et al., 2004
). Taken together, our data suggest that integrin signalling may enable undifferentiated neural progenitors to `read' the concentration of growth factors present in the extracellular environment with an increased sensitivity. This synergy between integrin and growth factor receptor signalling, also described in other systems (Schwartz and Ginsberg, 2002
; Yamada and Even-Ram, 2002
), is likely to become particularly important under physiological conditions when the availability of growth factors is limiting. Although the loss of ß1-integrin expression reduced the numbers of nestin-positive cells, in the absence of growth factors, it did not influence cell fate choice as the number of mutant and control spheres containing differentiated GFAP- and ß-III-tubulin-positive cells was not significantly different (data not shown).
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Addition of exogenous growth factors significantly increased the migration on laminin of both control (7.2±0.6; Fig. 6A,I) and mutant neurosphere cells (2.2±0.5; Fig. 6A,L) but failed to do so on fibronectin (Fig. 6A,H,K) or on PDL substrates (Fig. 6A,J,M). This suggests that at least on fibronectin substrates, ß1-integrin signalling is an absolute requirement for neurosphere cell migration.
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Discussion |
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We have suggested previously (Campos et al., 2004) that neural stem cell self-renewal is partially regulated by integrin and growth factor signalling through the MAPK pathway. We hypothesized that cross-talk between integrins and growth factors would allow neural stem cells to adjust better to changing local micro-environmental conditions during the development of the CNS. In the present study, we show that ß1-integrin signalling is not an absolute requirement for neural stem cell renewal. Control and ß1-deficient cells, plated at clonal density, showed no significant differences in the potential to form multipotent neurospheres over several passages. This was also true for limiting EGF concentrations. Furthermore, in assays where we followed the ratio of mutant (ß-gal positive)/wild-type (ß-gal negative) spheres within the same neurosphere culture over the course of at least five passages (more than 50 days in culture), no significant variations were found. We conclude that, at least under these culture conditions, control neural stem cells did not have a competitive advantage over their ß1 integrin-deficient counterparts.
In contrast to these results, the analysis of neurosphere size showed a significant increase in the percentage of small spheres and a decrease in large size spheres in ß1-deficient cultures. This size difference was accompanied by a significant reduction in the percentage of nestin-positive progenitors in mutant spheres. In mutant spheres, the percentage of proliferating cells was also reduced and the percentage of apoptotic progenitor cells increased. This effect appeared to mainly affect the undifferentiated progenitors, as the relative contribution of nestin-negative cells to the total number of dying or proliferating cells was low. Integrin signalling and its close cooperation with growth factor signalling has been linked to the regulation of both proliferation and apoptosis in a variety of different cell types (Yamada and Even-Ram, 2002). For example, in epithelial cells, the integrin
5ß1 mediates fibronectin-dependent cell proliferation through EGF receptor activation (Kuwada and Li, 2000
), and in skin fibroblasts ß1 integrins are able to activate the EGF receptor leading to ERK-1/MAPK induction and increased cell survival (Moro et al., 1998
). Integrin-mediated attachment to the ECM is a general requirement for cell survival in a variety of cells (Frisch and Francis, 1994
; Frisch and Ruoslahti, 1997
). Integrins can activate survival pathways via the PI 3-kinase and MAPK pathways and act as essential factors for their stimulation by growth factors (Stupack and Cheresh, 2002
). It seems conceivable therefore, that the increase in apoptotic progenitor cells in the mutant neurospheres is a direct consequence of the lack of ß1 integrin-mediated growth factor signal integration. To test this hypothesis, we grew mutant and control spheres in the absence of exogenous growth-factors, FGF-2 and EGF, and predicted that in these conditions the difference in the number of nestin progenitors between control and mutant neurospheres would be even greater than that observed in the presence of growth factors. Mutant and control spheres were plated on adhesive substrates and the number of neurospheres containing nestin-positive cells quantified after 6 days. We did indeed find that the ß1 integrin-deficient cells were much more sensitive than controls to the lack of exogenous growth factors as only 7.5% of the mutant neurosphere explants, in comparison with 96.8% of the control neurospheres, contained nestin-positive progenitors. It should be noted however, that the two neurosphere populations already showed differences in their cellular composition at the time of plating (see Fig. 4B). In the presence of low concentrations of exogenous growth factors, the number of mutant neurospheres containing nestin-positive progenitors increased to 85.1%, suggesting that in this context, ß1-integrin signalling synergizes with growth factor signalling to promote neural progenitor cell survival.
There are two phases of cell death in the developing CNS. The first phase occurs within progenitor cell populations to control the numbers of precursors. Abnormalities in this control result in the production of supranumerary progeny and leads to severe brain malformations (Roth et al., 2000). The second phase occurs later within post-mitotic neurons and oligodendrocytes in order to match their final numbers to the size of their target fields (Barres et al., 1992
; Colognato et al., 2002
; Haydar et al., 1999
; Kuan et al., 2000
). These two regulatory steps have different mechanisms of apoptosis with proteins of the Bcl family being involved in the cell death pathway of post-mitotic cells but not in that of progenitor populations (Haydar et al., 1999
; Kuan et al., 2000
). Signalling via ß1 integrin is known to play a role in target-dependent cell death (Colognato et al., 2002
; Gary et al., 2003
). Here, we show it also plays a role in the earlier phase of progenitor cell death. Our previous data on the distribution of ECM proteins within the subventricular zone (SVZ) (Campos et al., 2004
) together with the present data, strongly implicates ECM-ß1-integrin signalling as a regulator of this poorly understood mechanism of CNS size control.
Our data also provide evidence that ß1 integrins are important for the migration of neurosphere-derived cells on ECM substrates. Two observations justify this conclusion: first, we detected a significantly enhanced migration of these cells on fibronectin and laminin-1 as compared with PDL suggesting an important role for integrin-ECM interaction in the migration of neurosphere-derived cells; second, we observed a significantly impaired migration of ß1 integrin-deficient cells on both fibronectin and laminin-1 substrates. This effect was already visible 24 hours after plating and became more pronounced at later stages. Although on laminin-1 substrates, the addition of low amounts of growth factors elicited a modest increase in the extent of migration of mutant cells, it failed to do so on fibronectin. This indicates that expression of an integrin receptor containing a ß1 chain, probably the integrin fibronectin receptor 5ß1 expressed by neurospheres (Jacques et al., 1998
), is an absolute requirement for the migration of neurosphere cells on fibronectin.
There are several reports concerning the role of ß1 integrins in the migration of neuroepithelial cells in vivo. Galileo and colleagues (Galileo et al., 1992) reported that the radial migration of chicken tectal neuroepithelial cells is impaired following their infection with a retrovirus carrying the ß1-integrin cDNA in antisense orientation. On the other hand, Graus-Porta and colleagues (Graus-Porta et al., 2001
) have shown in an elegant loss-of-function study in vivo, that the lack of ß1 integrin appears not to affect autonomous migration of neural progenitors in the developing cortex and cerebellum of the mouse. In other in vivo studies, ß1-integrin signalling has been implicated in the regulation of tangential migration of olfactory interneuron precursors (Murase and Horwitz, 2002
). Cell migration from neurosphere explants cultured on ECM substrates does not mimic radial migration. In contrast to radial migration, where neural precursors migrate along radial glial processes, neurosphere-derived cells migrate as single cells in direct contact with the substrate. In this context, and in line with our results, it has been shown that the extent of neurosphere cell migration is influenced by the composition of the ECM (Kearns et al., 2003
) and that ß1-integrin signalling also plays an important role in the autonomous cell migration of different CNS cell types (Milner and Campbell, 2002
; Milner et al., 1996
; Schmid and Anton, 2003
).
In summary, by using neurosphere cultures, we were able to generate ß1 integrin-deficient cells to address the role of this protein in stem and neural progenitor cell biology. This was instrumental to circumvent the problem we encountered when trying to eliminate ß1 integrin from the cell surface of recombined cells during early embryonic development (our unpublished results). The slow turnover of ß1 integrin following recombination of the conditional allele prevented the loss of the protein in a time frame fitting with our in vivo experimental requirements. Our in vitro study is limited in that the composition of the ECM present within neurospheres might differ from the ECM composition in the ventricular zone. Nevertheless, neurospheres express some of the ECM molecules seen in vivo, and to a certain extent, the three-dimensional distribution of the ECM within the neurospheres reflects that of the ventricular zone (Campos et al., 2004). Therefore, it is conceivable that the ß1 integrin-dependent regulation of undifferentiated progenitor cell behaviour might also operate in vivo during normal development or following injury to the CNS. In relation to CNS injury, Picard-Riera and colleagues have recently shown that during experimental autoimmune encephalomyelitis, where the ECM composition and growth factor availability go through dynamic changes, progenitor cells present in the SVZ increase their proliferation index before migrating into the periventricular white matter to give rise to oligodendrocytes and astrocytes (Picard-Riera et al., 2002
). It will be interesting to determine if these processes are dependent upon ß1 integrin.
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Acknowledgments |
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Footnotes |
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Present address: Department of Biological Sciences, 385 Serra Mall, Stanford University, Stanford, CA 94305, USA
Present address: The Wellcome Trust Sanger Institute, Hinxton Hall, Cambridge, CB10 1SA, UK
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References |
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![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Adams, J. C. and Watt, F. M. (1993). Regulation of development and differentiation by the extracellular matrix. Development 117, 1183-1198.
Anderson, D. J. (2001). Stem cells and pattern formation in the nervous system. The possible versus the actual. Neuron 30, 19-35.[CrossRef][Medline]
Aplin, A. E., Short, S. M. and Juliano, R. L. (1999). Anchorage-dependent regulation of the mitogen-activated protein kinase cascade by growth factors is supported by a variety of integrin alpha chains. J. Biol. Chem. 274, 31223-31228.
Barres, B. A., Hart, I. K., Coles, H. S., Burne, J. F., Voyvodic, J. T., Richardson, W. D. and Raff, M. C. (1992). Cell death and control of cell survival in the oligodendrocyte lineage. Cell 70, 31-46.[CrossRef][Medline]
Brakebusch, C., Grose, R., Quondamatteo, F., Ramirez, A., Jorcano, J. L., Pirro, A., Svensson, M., Herken, R., Sasaki, T., Timpl, R. et al. (2000). Skin and hair follicle integrity is crucially dependent on beta 1 integrin expression on keratinocytes. EMBO J. 19, 3990-4003.
Bronner-Fraser, M. (1993). Mechanisms of neural crest cell migration. BioEssays 15, 221-230.[CrossRef][Medline]
Brooks, P. C., Klemke, R. L., Schon, S., Lewis, J. M., Schwartz, M. A. and Cheresh, D. A. (1997). Insulin-like growth factor receptor cooperates with integrin alpha v beta 5 to promote tumor cell dissemination in vivo. J. Clin. Invest. 99, 1390-1398.
Campos, L. S., Leone, D. P., Relvas, J. B., Brakebusch, C., Fassler, R., Suter, U. and ffrench-Constant, C. (2004). {beta}1 integrins activate a MAPK signalling pathway in neural stem cells that contributes to their maintenance. Development 131, 3433-3444.
Ciccolini, F. and Svendsen, C. N. (1998). Fibroblast growth factor 2 (FGF-2) promotes acquisition of epidermal growth factor (EGF) responsiveness in mouse striatal precursor cells: identification of neural precursors responding to both EGF and FGF-2. J. Neurosci. 18, 7869-7880.
Clegg, D. O., Wingerd, K. L., Hikita, S. T. and Tolhurst, E. C. (2003). Integrins in the development, function and dysfunction of the nervous system. Front. Biosci. 8, d723-d750.[Medline]
Collins, A. T., Habib, F. K., Maitland, N. J. and Neal, D. E. (2001). Identification and isolation of human prostate epithelial stem cells based on alpha(2)beta(1)-integrin expression. J. Cell Sci. 114, 3865-3872.
Colognato, H., Baron, W., Avellana-Adalid, V., Relvas, J. B., Baron-Van Evercooren, A., Georges-Labouesse, E. and ffrench-Constant, C. (2002). CNS integrins switch growth factor signalling to promote target-dependent survival. Nat. Cell Biol. 4, 833-841.[CrossRef][Medline]
Danen, E. H. and Sonnenberg, A. (2003). Integrins in regulation of tissue development and function. J. Pathol. 200, 471-480.[CrossRef][Medline]
Drago, J., Nurcombe, V. and Bartlett, P. F. (1991). Laminin through its long arm E8 fragment promotes the proliferation and differentiation of murine neuroepithelial cells in vitro. Exp. Cell Res. 192, 256-265.[CrossRef][Medline]
Fassler, R. and Meyer, M. (1995). Consequences of lack of beta 1 integrin gene expression in mice. Genes Dev. 9, 1896-1908.[Abstract]
Frederiksen, K. and McKay, R. D. (1988). Proliferation and differentiation of rat neuroepithelial precursor cells in vivo. J. Neurosci. 8, 1144-1151.[Abstract]
Frisch, S. M. and Francis, H. (1994). Disruption of epithelial cell-matrix interactions induces apoptosis. J. Cell Biol. 124, 619-626.[Abstract]
Frisch, S. M. and Ruoslahti, E. (1997). Integrins and anoikis. Curr. Opin. Cell Biol. 9, 701-706.[CrossRef][Medline]
Gaiano, N. and Fishell, G. (2002). The role of notch in promoting glial and neural stem cell fates. Annu. Rev. Neurosci. 25, 471-490.[CrossRef][Medline]
Galileo, D. S., Majors, J., Horwitz, A. F. and Sanes, J. R. (1992). Retrovirally introduced antisense integrin RNA inhibits neuroblast migration in vivo. Neuron 9, 1117-1131.[CrossRef][Medline]
Gary, D. S., Milhavet, O., Camandola, S. and Mattson, M. P. (2003). Essential role for integrin linked kinase in Akt-mediated integrin survival signaling in hippocampal neurons. J. Neurochem. 84, 878-890.[CrossRef][Medline]
Georges-Labouesse, E., Mark, M., Messaddeq, N. and Gansmuller, A. (1998). Essential role of alpha 6 integrins in cortical and retinal lamination. Curr. Biol. 8, 983-986.[CrossRef][Medline]
Gilmore, A. P., Metcalfe, A. D., Romer, L. H. and Streuli, C. H. (2000). Integrin-mediated survival signals regulate the apoptotic function of Bax through its conformation and subcellular localization. J. Cell Biol. 149, 431-446.
Graus-Porta, D., Blaess, S., Senften, M., Littlewood-Evans, A., Damsky, C., Huang, Z., Orban, P., Klein, R., Schittny, J. C. and Muller, U. (2001). Beta1-class integrins regulate the development of laminae and folia in the cerebral and cerebellar cortex. Neuron 31, 367-379.[CrossRef][Medline]
Gritti, A., Parati, E. A., Cova, L., Frolichsthal, P., Galli, R., Wanke, E., Faravelli, L., Morassutti, D. J., Roisen, F., Nickel, D. D. et al. (1996). Multipotential stem cells from the adult mouse brain proliferate and self-renew in response to basic fibroblast growth factor. J. Neurosci. 16, 1091-1100.[Abstract]
Gurley, L. R., D'Anna, J. A., Barham, S. S., Deaven, L. L. and Tobey, R. A. (1978). Histone phosphorylation and chromatin structure during mitosis in Chinese hamster cells. Eur. J. Biochem. 84, 1-15.[CrossRef][Medline]
Haydar, T. F., Kuan, C. Y., Flavell, R. A. and Rakic, P. (1999). The role of cell death in regulating the size and shape of the mammalian forebrain. Cereb. Cortex 9, 621-626.
Hendzel, M. J., Wei, Y., Mancini, M. A., Van Hooser, A., Ranalli, T., Brinkley, B. R., Bazett-Jones, D. P. and Allis, C. D. (1997). Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation. Chromosoma 106, 348-360.[CrossRef][Medline]
Hirsch, E., Barberis, L., Brancaccio, M., Azzolino, O., Xu, D., Kyriakis, J. M., Silengo, L., Giancotti, F. G., Tarone, G., Fassler, R. et al. (2002). Defective Rac-mediated proliferation and survival after targeted mutation of the beta1 integrin cytodomain. J. Cell Biol. 157, 481-492.
Hockfield, S. and McKay, R. D. (1985). Identification of major cell classes in the developing mammalian nervous system. J. Neurosci. 5, 3310-3328.[Abstract]
Hynes, R. O. (1992). Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69, 11-25.[CrossRef][Medline]
Hynes, R. O. (1996). Targeted mutations in cell adhesion genes: what have we learned from them? Dev. Biol. 180, 402-412.[CrossRef][Medline]
Imura, T., Kornblum, H. I. and Sofroniew, M. V. (2003). The predominant neural stem cell isolated from postnatal and adult forebrain but not early embryonic forebrain expresses GFAP. J. Neurosci. 23, 2824-2832.
Jacques, T. S., Relvas, J. B., Nishimura, S., Pytela, R., Edwards, G. M., Streuli, C. H. and ffrench-Constant, C. (1998). Neural precursor cell chain migration and division are regulated through different beta1 integrins. Development 125, 3167-3177.
Jones, P. H. and Watt, F. M. (1993). Separation of human epidermal stem cells from transit amplifying cells on the basis of differences in integrin function and expression. Cell 73, 713-724.[CrossRef][Medline]
Jones, P. H., Harper, S. and Watt, F. M. (1995). Stem cell patterning and fate in human epidermis. Cell 80, 83-93.[CrossRef][Medline]
Kalamarides, M., Niwa-Kawakita, M., Leblois, H., Abramowski, V., Perricaudet, M., Janin, A., Thomas, G., Gutmann, D. H. and Giovannini, M. (2002). Nf2 gene inactivation in arachnoidal cells is rate-limiting for meningioma development in the mouse. Genes Dev. 16, 1060-1065.
Kearns, S. M., Laywell, E. D., Kukekov, V. K. and Steindler, D. A. (2003). Extracellular matrix effects on neurosphere cell motility. Exp. Neurol. 182, 240-244.[CrossRef][Medline]
Kuan, C. Y., Roth, K. A., Flavell, R. A. and Rakic, P. (2000). Mechanisms of programmed cell death in the developing brain. Trends Neurosci. 23, 291-297.[CrossRef][Medline]
Kuwada, S. K. and Li, X. (2000). Integrin alpha5/beta1 mediates fibronectin-dependent epithelial cell proliferation through epidermal growth factor receptor activation. Mol. Cell. Biol. 11, 2485-2496.
Lendahl, U., Zimmerman, L. B. and McKay, R. D. (1990). CNS stem cells express a new class of intermediate filament protein. Cell 60, 585-595.[CrossRef][Medline]
Lobo, M. V., Alonso, F. J., Redondo, C., Lopez-Toledano, M. A., Caso, E., Herranz, A. S., Paino, C. L., Reimers, D. and Bazan, E. (2003). Cellular characterization of epidermal growth factor-expanded free-floating neurospheres. J. Histochem. Cytochem. 51, 89-103.
Lutolf, S., Radtke, F., Aguet, M., Suter, U. and Taylor, V. (2002). Notch1 is required for neuronal and glial differentiation in the cerebellum. Development 129, 373-385.[Medline]
McKay, R. (1997). Stem cells in the central nervous system. Science 276, 66-71.
Milner, R. and Campbell, I. L. (2002). The integrin family of cell adhesion molecules has multiple functions within the CNS. J. Neurosci. Res. 69, 286-391.[CrossRef][Medline]
Milner, R., Edwards, G., Streuli, C. and Ffrench-Constant, C. (1996). A role in migration for the alpha V beta 1 integrin expressed on oligodendrocyte precursors. J. Neurosci. 16, 7240-7252.
Milner, R., Relvas, J. B., Fawcett, J. and ffrench-Constant, C. (2001). Developmental regulation of alphav integrins produces functional changes in astrocyte behavior. Mol. Cell Neurosci. 18, 108-118.[CrossRef][Medline]
Montgomery, A. M., Becker, J. C., Siu, C. H., Lemmon, V. P., Cheresh, D. A., Pancook, J. D., Zhao, X. and Reisfeld, R. A. (1996). Human neural cell adhesion molecule L1 and rat homologue NILE are ligands for integrin alpha v beta 3. J. Cell Biol. 132, 475-485.[Abstract]
Moro, L., Venturino, M., Bozzo, C., Silengo, L., Altruda, F., Beguinot, L., Tarone, G. and Defilippi, P. (1998). Integrins induce activation of EGF receptor: role in MAP kinase induction and adhesion-dependent cell survival. EMBO J. 17, 6622-6632.
Murase, S. and Horwitz, A. F. (2002). Deleted in colorectal carcinoma and differentially expressed integrins mediate the directional migration of neural precursors in the rostral migratory stream. J. Neurosci. 22, 3568-3579.
Ohtsuka, T., Sakamoto, M., Guillemot, F. and Kageyama, R. (2001). Roles of the basic helix-loop-helix genes Hes1 and Hes5 in expansion of neural stem cells of the developing brain. J. Biol. Chem. 276, 30467-30474.
Paulson, J. R. and Taylor, S. S. (1982). Phosphorylation of histones 1 and 3 and nonhistone high mobility group 14 by an endogenous kinase in HeLa metaphase chromosomes. J. Biol. Chem. 257, 6064-6072.
Picard-Riera, N., Decker, L., Delarasse, C., Goude, K., Nait-Oumesmar, B., Liblau, R., Pham-Dinh, D. and Evercooren, A. B. (2002). Experimental autoimmune encephalomyelitis mobilizes neural progenitors from the subventricular zone to undergo oligodendrogenesis in adult mice. Proc. Natl. Acad. Sci. USA 99, 13211-13216.
Potocnik, A. J., Brakebusch, C. and Fassler, R. (2000). Fetal and adult hematopoietic stem cells require beta1 integrin function for colonizing fetal liver, spleen, and bone marrow. Immunity 12, 653-663.[CrossRef][Medline]
Rao, M. S. (1999). Multipotent and restricted precursors in the central nervous system. Anat. Rec. 257, 137-148.[CrossRef][Medline]
Reynolds, B. A. and Weiss, S. (1992). Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255, 1707-1710.[Medline]
Reynolds, B. A. and Weiss, S. (1996). Clonal and population analyses demonstrate that an EGF-responsive mammalian embryonic CNS precursor is a stem cell. Dev. Biol. 175, 1-13.[CrossRef][Medline]
Reynolds, B. A., Tetzlaff, W. and Weiss, S. (1992). A multipotent EGF-responsive striatal embryonic progenitor cell produces neurons and astrocytes. J. Neurosci. 12, 4565-4574.[Abstract]
Roth, K. A., Kuan, C., Haydar, T. F., D'Sa-Eipper, C., Shindler, K. S., Zheng, T. S., Kuida, K., Flavell, R. A. and Rakic, P. (2000). Epistatic and independent functions of caspase-3 and Bcl-X(L) in developmental programmed cell death. Proc. Natl. Acad. Sci. USA 97, 466-471.
Ruppert, M., Aigner, S., Hubbe, M., Yagita, H. and Altevogt, P. (1995). The L1 adhesion molecule is a cellular ligand for VLA-5. J. Cell Biol. 131, 1881-1891.[Abstract]
Sastry, S. K., Lakonishok, M., Thomas, D. A., Muschler, J. and Horwitz, A. F. (1996). Integrin alpha subunit ratios, cytoplasmic domains, and growth factor synergy regulate muscle proliferation and differentiation. J. Cell Biol. 133, 169-184.[Abstract]
Schmid, R. S. and Anton, E. S. (2003). Role of integrins in the development of the cerebral cortex. Cereb. Cortex 13, 219-224.
Schwartz, M. A. and Ginsberg, M. H. (2002). Networks and crosstalk: integrin signalling spreads. Nat. Cell Biol. 4, E65-E68.[CrossRef][Medline]
Shinohara, T., Avarbock, M. R. and Brinster, R. L. (1999). beta1- and alpha6-integrin are surface markers on mouse spermatogonial stem cells. Proc. Natl. Acad. Sci. USA 96, 5504-5509.
Stupack, D. G. and Cheresh, D. A. (2002). Get a ligand, get a life: integrins, signaling and cell survival. J. Cell Sci. 115, 3729-3738.
Svendsen, C. N., Fawcett, J. W., Bentlage, C. and Dunnett, S. B. (1995). Increased survival of rat EGF-generated CNS precursor cells using B27 supplemented medium. Exp. Brain Res. 102, 407-414.[Medline]
Tekki-Kessaris, N., Woodruff, R., Hall, A. C., Gaffield, W., Kimura, S., Stiles, C. D., Rowitch, D. H. and Richardson, W. D. (2001). Hedgehog-dependent oligodendrocyte lineage specification in the telencephalon. Development 128, 2545-2554.
Tropepe, V., Sibilia, M., Ciruna, B. G., Rossant, J., Wagner, E. F. and van der Kooy, D. (1999). Distinct neural stem cells proliferate in response to EGF and FGF in the developing mouse telencephalon. Dev. Biol. 208, 166-188.[CrossRef][Medline]
Vescovi, A. L., Reynolds, B. A., Fraser, D. D. and Weiss, S. (1993). bFGF regulates the proliferative fate of unipotent (neuronal) and bipotent (neuronal/astroglial) EGF-generated CNS progenitor cells. Neuron 11, 951-966.[CrossRef][Medline]
Weiss, S., Reynolds, B. A., Vescovi, A. L., Morshead, C., Craig, C. G. and van der Kooy, D. (1996). Is there a neural stem cell in the mammalian forebrain? Trends Neurosci. 19, 387-393.[CrossRef][Medline]
Yamada, K. M. and Even-Ram, S. (2002). Integrin regulation of growth factor receptors. Nat. Cell Biol. 4, E75-E76.[CrossRef][Medline]
Zhang, Z., Vuori, K., Reed, J. C. and Ruoslahti, E. (1995). The alpha 5 beta 1 integrin supports survival of cells on fibronectin and up-regulates Bcl-2 expression. Proc. Natl. Acad. Sci. USA 92, 6161-6165.
Zhu, A. J., Haase, I. and Watt, F. M. (1999a). Signaling via beta1 integrins and mitogen-activated protein kinase determines human epidermal stem cell fate in vitro. Proc. Natl. Acad. Sci. USA 96, 6728-6733.
Zhu, G., Mehler, M. F., Mabie, P. C. and Kessler, J. A. (1999b). Developmental changes in progenitor cell responsiveness to cytokines. J. Neurosci. Res. 56, 131-145.[CrossRef][Medline]