1 Gut Barrier Group, Injury Research, Salford Royal Hospitals NHS Trust and Faculty of Medicine and Human Sciences, University of Manchester, Clinical Sciences Building, Hope Hospital, Salford, M6 8HD, UK
2 Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester, M13 9PT, UK
* Author for correspondence (e-mail: gwarhurs{at}fs1.ho.man.ac.uk)
Accepted 11 August 2005
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Summary |
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Key words: Inflammation, Cytokine, Tight junction, Intestine, Epithelial
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Introduction |
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Evidence from both cultured cell and animal models suggests that the pro-inflammatory cytokine, interferon- (IFN
) may act as a key modulator of intestinal barrier function (Walsh et al., 2000
; Ferrier et al., 2003
). Incubation of colonic epithelial cell monolayers with IFN
alone (Madara and Stafford, 1989
; Youakim and Ahdieh, 1999
) or in combination with other cytokines (Han et al., 2003
; Bruewer et al., 2003
) elicits dramatic reductions in transepithelial resistance (TER) along with increased solute permeability. These effects are mediated at the molecular level by altered expression and/or redistribution of tight junction proteins and perturbations of the actin cytoskeleton (Youakim and Ahdieh, 1999
; Bruewer et al., 2003
). Recent evidence suggests that IFN
-mediated inhibition of epithelial Na+,K+-ATPase activity plays a critical role in initiating the signalling pathways that lead to barrier disruption (Sugi et al., 2001
; Bertelsen et al., 2004
). Despite these studies, our understanding of how IFN
modulates the paracellular pore at the functional level, and how this might affect intestinal permeability to antigens, remains limited. One unresolved question is whether this cytokine causes non-specific disruption of tight junctions or elicits more subtle effects on junctional permeability to facilitate antigen entry.
There is an increasing realisation that the molecular complexity of tight junctions (D'Atri and Citi, 2002; Rahner et al., 2001
) may be the basis for a functional complexity involving multiple populations of size- and charge-limiting pores that could allow different physiological stimuli to induce specific effects on paracellular permeability (Karczewski and Groot, 2000
). However, the poor resolution and sensitivity inherent in conventional approaches to measuring paracellular function have limited our ability to identify functional heterogeneity. We have recently described a functional profiling technique that simultaneously measures the transepithelial permeability of 24 PEG oligomers of defined molecular radius by mass spectrometry (Watson et al., 2001
). When analysed by a modified sieving model, this approach resolves distinct populations of paracellular pores in colonic epithelial cells that appear to be differentially regulated by tight junction modulators such as EGTA and sodium caprate (Watson et al., 2001
). The aim of the present study was to utilise this approach to provide a detailed insight into the functional modulation of the paracellular route by IFN
in gut epithelial cells.
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Materials and Methods |
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T84 cell culture
T84 cells (passages 98-120) were maintained in a 1:1 mixture of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 medium supplemented with 14 mM NaHCO3, 50 IU penicillin and 50 µg/ml streptomycin in 25cm2 flasks at 37°C in a 5% CO2 constant humidity environment with medium replaced three times a week. For transport experiments and immunoblot analysis, cells were seeded on 0.4 µm pore size Snapwell or Transwell culture inserts respectively (Costar, High Wycombe, UK). Inserts were seeded with 2-3x105 cells/cm2 and were grown for at least 21 days before use in experiments, to allow development of monolayers with a high (>1200 ·cm2) and stable transepithelial resistance (TER). Development of TER was monitored using an Evometer (World Precision Instruments) fitted with chopstick electrodes.
Treatment with IFN
T84 monolayers were exposed to IFN at various concentrations (0.01-10 ng/ml) on the basolateral surface for 4-48 hours in culture medium. Changes in TER were monitored throughout using the Evometer. At the end of this period, culture medium was removed, the cells were washed with HBSS at 37°C and either mounted in Ussing chambers for transport studies or processed for immunoblotting of tight junction proteins. In some instances, cells were allowed to recover from IFN
treatment by washing three times in medium followed by maintenance in complete culture medium without IFN
for the specified times.
Transport studies
T84 monolayers were removed from the growth medium and washed twice in prewarmed HBSS (supplemented with 20 mM HEPES). Monolayers were subsequently placed in a modified Ussing chamber (1.0 cm2 diffusion window) and equilibrated with HBSS on both apical and basolateral surfaces. Chambers were maintained at 37°C and under constant O2. Spontaneous tissue potential difference (PD) and TER, measured as deflection in PD caused by a 100 µA current pulse, were monitored throughout the experiment. After the equilibration period the paracellular markers PEG400/1000 (2 µg/ml), [14C]mannitol (0.2 µCi/ml; 100 µM), 10 kDa FITC-dextran (10 µM; FD10) were added to the apical (donor) chamber. A 100 µl sample was removed from the donor chamber to determine the initial concentration. Samples were removed from the receiver chamber at 30 minute intervals and replaced with fresh buffer. Permeability to bacterial lipopolysaccharide was assessed by adding FITC-LPS (0.2 mg/ml from Escherichia coli 0111:B4) to the apical chamber and the basolateral medium sampled after 4 hours. Concentrations of mannitol were assessed by liquid scintillation counting and FD10 and FITC-LPS by fluorimetry (excitation 485 nm/emission 530 nm) respectively. PEG samples were stored at 70°C until analysed by LC-MS. The detailed protocol for resolution of PEG oligomers by LC-MS has been described previously (Watson et al., 2001).
Permeability values for mannitol, PEG oligomers and FD10 are expressed as apparent permeability (Papp) in cm/second, obtained according to the equation:
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Mathematical modelling of paracellular permeation
The resolution of the polydisperse mixture of PEG 400/1000 by LC-MS allows the paracellular permeability of 24 polyethylene glycols of increasing molecular mass (0.238-1.25 kDa) and molecular radius (3.5-7.4 Å) to be measured simultaneously. These data provide a profile of paracellular permeability over a range of molecular sizes that can be modelled using a modified sieving model (Watson et al., 2001).
Briefly, this model describes PEG permeability across epithelial monolayers as a biphasic process with a restrictive component (C1) where PEG permeability declines with increasing molecular radius and a second component (C2) where no size restriction is apparent for this size-range of markers. The sieving model describes the pore radius of C1 as rs1, which is the point at which no permeation occurs through C1 when the radius of the oligomer, r, equals or exceeds a critical value, rs1 (measured in Å). In addition, the model defines two other parameters (units of second1) which is directly proportional to the number of restrictive tight junction pores and ß (units of cm2/second), which is directly proportional to both the number and the radius of the non-restrictive tight junction pores. These parameters are defined in the following equation, the derivation of which is described in Watson et al. (Watson et al., 2001
):
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Internalisation studies
To investigate the possible effects of IFN on probe internalisation, control or IFN
-treated (10 ng/ml; 48 hours) monolayers were equilibrated in HBSS and incubated on the apical surface with HBSS containing 1 mg/ml Rhodamine-labelled, lysine-fixable 10 kDa dextran for 60 minutes at 37°C under conditions which replicated the permeability studies using FITC-dextran. At the end of this period, monolayers were washed three times with HBSS at 4°C, fixed with 4% paraformaldehyde and permeabilised with 0.1% Triton X-100. After washing with PBS, all samples were stained with the nuclear stain DAPI (4',6-diamidino-2-phenylindole dihydrochloride; 0.2% in PBS) for 5 minutes at 4°C, followed by further washing in PBS and mounting on slides with Vectashield (Vector Laboratories, Peterborough, UK). Studies of the effect of extracellular calcium depletion on dextran internalisation used a modification of the method of Ivanov et al. (Ivanov et al., 2004
). Monolayers were washed repeatedly in calcium-free Eagle's minimal essential medium for suspension culture (S-MEM; Gibco) supplemented with 2 mM EGTA, 10 mM HEPES, 14 mM NaHCO3 and 50 IU penicillin/50 µg/ml streptomycin and 10% foetal calf serum at 4°C. One mg/ml Rhodamine-dextran in pre-warmed S-MEM was then placed on both apical and basolateral surfaces and the monolayers incubated for 120 minutes at 37°C. Monolayers were subsequently washed, fixed and permeabilised as described above.
Stained monolayers were analysed by confocal laser-scanning microscopy on a Biorad MRC1024 confocal system mounted on a Nikon Eclipse TE300 fluorescence microscope with a X40 Plan Fluor oil objective. Images shown are representative of three similar monolayers with multiple images taken from each preparation.
In other studies, control and IFN-treated (10 ng/ml) monolayers were incubated on the apical surface with HBSS containing 14C-PEG4000 (1 µCi/ml) for 60 minutes. At the end of the incubation, the radio-labelled PEG4000 was removed and the monolayers washed three times rapidly in an excess of ice-cold HBSS. Washed monolayers were placed in an emulsifying cocktail (Ultima Gold, Perkin-Elmer, Beaconsfield, UK) for scintillation counting.
Immunoblotting of tight junction proteins
T84 monolayers were lysed at 4°C by scraping into a buffer containing 120 mM NaCl, 25 mM HEPES (pH 7.5), 2 mM EDTA, 25 mM NaF, 1 mM NaVO4, 1% (w/v) Triton X-100 and 5% (v/v) protease inhibitor cocktail (Sigma). Samples were left on ice for 30 minutes after which detergent-insoluble material was pelleted by centrifugation at 12,000 g for 30 minutes at 4°C. The supernatant (detergent-soluble fraction) was retained. The detergent-insoluble pellet was solubilised in a buffer containing 25 mM HEPES (pH 7.5), 2 mM EDTA, 25 mM NaF, 1 mM NaVO4, 5% (v/v) protease inhibitor cocktail and 1% (w/v) sodium dodecyl sulphate (SDS) by sonication for 3 seconds on ice. The protein content of both detergent soluble and insoluble fractions was determined by the BCA method (Pierce, USA). Fractions were stored at 80°C until use.
Detergent soluble and insoluble fractions (20 µg/well) were separated by SDS-PAGE on either 7.5% (occludin) or 12% (claudin) polyacrylamide gels. After electrophoretic transfer to nitrocellulose membranes (Hybond), membranes were blocked at 4°C overnight in TBS-T (50 mM Tris-HCl pH 7.9, 150 mM NaCl and 0.05% v/v Tween 20) containing 2% (w/v) milk protein. Membranes were washed in TBS-T and probed with polyclonal antibodies to claudin-1, claudin-2 or claudin-3 or a monoclonal antibody to occludin (all at 1:1000 dilution in TBS-T) for 1 hour. After washing and incubation with the appropriate horseradish peroxidase-conjugated secondary antibody (1:5000), protein bands were visualised using the ECL system (Amersham Pharmacia, Bucks, UK) followed by exposure of blots to X-OMAT film (Kodak-Eastman). Where indicated, immunoblot images were scanned and quantified densitometrically using Scion Image software (Scion Corporation, Frederick, USA). In all cases, membranes were also stained with Coomassie Blue to verify equal protein loading in each well.
Statistical analysis
Results are expressed as means±s.e.m. for the indicated number of observations (n). Data were analysed by ANOVA with post hoc Bonferroni or paired t-tests as appropriate. A P value of 0.05 was accepted as the level of statistical significance. All analyses were undertaken using Graphpad Prism software (Graphpad Prism, San Diego, California, USA).
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Results |
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The IFN-induced changes in PEG permeability were further analysed using the two-component sieving model (Table 1). This provides further evidence that the primary effect of the cytokine is a preferential increase in the paracellular permeability to larger molecules. Values for both rsl, an estimate of the diameter of the restrictive pore and
, which is proportional to the number of restrictive pores were unchanged in IFN
-treated monolayers. In contrast, values for ß, the descriptor of the non-restrictive component of PEG flux increased markedly, being 15-fold higher in cells exposed to 10 ng/ml IFN
for 48 hours. Fig. 2 compares changes in
and ß with the increase in permeability of a large paracellular probe, 10 kDa dextran, following treatment of T84 cells with IFN
. Values for
show no significant change in response to IFN
even at concentrations up to 100 ng/ml, whereas ß values increase dramatically (Fig. 2A). These effects were accompanied by a marked increase in permeability of the 10 kDa paracellular probe FD10 (Fig. 2B). Indeed, there was a strong correlation between changes in ß and FD10 permeability (coefficient of determination r2=0.913; P<0.05) with similar fold increases (15- and 21-fold respectively). The EC50 values for IFN
activation of these two parameters were also similar (3.6 and 2.2 ng/ml respectively). Changes in ß also appeared to be associated with the IFN
-induced decrease in TER (compare Fig. 2A and Fig. 1A), however, linear regression analysis did not show a statistically significant correlation between these two parameters (r2=0.817; P>0.05). This suggests that increases in the non-restrictive component of PEG flux rather than changes in the size or frequency of the restrictive paracellular pore are responsible for increased permeability to large molecules. The differential effect of IFN
on the permeability of FD10 compared to the much smaller paracellular probe mannitol provides further support for this hypothesis (Fig. 3).
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One possibility to be excluded was that the selective increase in permeability of larger molecules was due to IFN increasing their internalisation and transcellular passage across the monolayers. To address this, intracellular levels of dextran were imaged by confocal microscopy in control and IFN
-treated (10 ng/ml) monolayers following incubation on the apical surface with lysine-fixable, Rhodamine-labelled 10 kDa dextran under the same conditions as used in the permeability studies (Fig. 4). Small but significant levels of Rhodamine-dextran are found within the cells of the monolayers after 60 minutes of incubation (Fig. 4A) but there was no evidence for an increase in intracellular dextran following IFN
treatment (Fig. 4B). As a positive control, monolayers were also incubated under a condition that depletes extracellular Ca2+, which has been shown previously to stimulate endocytic activity in T84 cells (Ivanov et al., 2004
). This treatment caused a marked increase in intracellular Rhodamine-dextran staining (Fig. 4C).
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Incubation of T84 monolayers for 60 minutes with 14C-PEG4000 followed by rapid washing and analysis of cell-associated radioactivity also showed no significant difference between control and IFN-treated (10 ng/ml) cells (Control: 0.018±0.002, compared to IFN
(10 ng/ml): 0.014±0.004% total 14C-PEG per 106 cells; n=3). Taken together these studies suggest that increased internalisation and transcellular passage are unlikely to be a significant factor in the 15- and 21-fold increases in the transepithelial permeability of PEG 1250 and dextran 10 kDa probes respectively, that follow exposure to IFN
. We next investigated whether the selective opening of a paracellular route for large molecules by IFN
is associated with an increased passage of antigenic macromolecules such as endotoxin. Untreated T84 monolayers showed a low level of FITC-LPS permeability, which was increased by almost tenfold (P<0.01) following exposure to 10 ng/ml IFN
(Fig. 5A). This effect followed IFN
-mediated changes in TER and was also insensitive to inhibitors of transcytotic pathways (Fig. 5B). Chlorpromazine (CPZ) an inhibitor of clathrin-mediated endocytic processes, LY-294002, an inhibitor of PI-3 kinase-mediated macropinocytosis and filipin, an inhibitor of lipid raft-mediated processes had no significant effect on the IFN
-mediated increase in FITC-LPS. At the concentrations used, these inhibitors had no effect on basal TER, however, higher concentrations of CPZ (20 µg/ml) and LY-294002 (25 µM) caused a reduction in TER and further increased flux of FITC-LPS (data not shown). These data are consistent with IFN
activating a paracellular route for the passage of large, potentially antigenic, molecules like LPS.
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To investigate further the molecular basis for differential effects of IFN on the restrictive and non-restrictive components of paracellular permeability, changes in the expression and localisation of tight junction proteins were studied (Fig. 6). Under control conditions, the transmembrane tight junction protein, occludin was expressed as a 65 kDa band in both soluble and insoluble fractions. However the insoluble pool also contained a heterogeneous mixture of polypeptides of higher molecular mass indicative of hyper-phosphorylated forms of the protein (Nishiyama et al., 2001
). IFN
treatment for 48 hours resulted in a dramatic decrease in the levels of occludin bands of higher molecular mass in the insoluble fraction. This was accompanied by a more modest reduction in the level of the main 65 kDa species. The effects of IFN
on the expression profile of three claudin species CLD1, CLD2 and CLD3 were markedly different (Fig. 6). Under control conditions, CLD1 was distributed primarily in the soluble fraction with smaller amounts in the insoluble pool. Following exposure to IFN
, there was a significant increase in the levels of CLD1 associated with the insoluble pool. In contrast, the expression of CLD3 expression did not appear to be influenced by IFN
and CLD2 was not consistently detected in these cells under either control or IFN
-stimulated conditions.
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Studies were also undertaken to assess the reversibility of the functional and molecular changes induced by IFN. The IFN
-induced decrease in TER was completely reversible such that 96 hours following IFN
removal, TER values were not significantly different from untreated monolayers (Control: 1260±65; 96 hours post IFN
: 1120±90
.cm2; n=4). Similarly, the PEG permeability profile of T84 monolayers 96 hours after IFN
washout were virtually identical to untreated monolayers although a consistent observation was that the permeability of the larger oligomers did not return fully to control levels (Fig. 7). As a result, whereas the 15-fold increase in ß caused by IFN
was largely reversed after 96 hours (Table 1), values remained slightly but significantly higher (P<0.05) than untreated controls. At the molecular level, the decreased expression of both 65 kDa and hyper-phosphorylated forms of occludin appeared to be completely reversed following removal of IFN
for 96 hours (Fig. 8). However, the IFN
-induced increase in claudin-1 in the insoluble fraction showed no evidence of reversal after 96 hours even though TER and PEG permeability had largely returned to control values by this time.
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Discussion |
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The molecular dimensions of the non-restrictive pathway cannot be estimated with the PEG oligomer series used here as the largest PEG oligomer (1250 kDa; hydrodynamic radius 7.4 Å) is smaller than other paracellular probes able to permeate across IFN-treated T84 cells. For example FD10 has a hydrodynamic radius of 23Å (Sanders et al., 1995
). However, as the data in Fig. 2 demonstrates, there is a very close correlation between the IFN
-mediated increase in FD10 permeability and IFN
-mediated changes in the value of ß, the descriptor of the non-restrictive pathway calculated from the PEG permeability profile. This supports the validity of using ß to assess the contribution of this pathway to the permeation of large molecules following exposure to IFN
. As noted previously (Watson et al., 2001
), in unstimulated T84 monolayers the value of ß is 15- to 20-fold lower than
indicating that under these conditions paracellular permeability is predominantly controlled by the restrictive pathway for small molecules. The dramatic (15-fold) rise in ß on exposure to IFN
suggests that the non-restrictive pathway will become a more significant component of junctional permeability in inflammatory conditions. This will have a proportionally greater impact on permeability to large compared to small molecules as evidenced by the relative increases in mannitol and FD10 permeation following treatment with 10 ng/ml IFN
(fourfold for mannitol compared to 21-fold for FD10).
Although the limitations of conventional paracellular probes have limited investigation of functional heterogeneity in tight junctions, several observations are consistent with the current findings. Bijlsma and co-workers (Bijlsma et al., 1996) showed that carbachol increased the paracellular flux of horseradish peroxidase across isolated rat ileum to a much greater extent than small probes like mannitol. The authors hypothesised that this was due to the appearance of large diameter pores caused by fusion of smaller pores within the tight junction. Interestingly, freeze fracture studies of HT29-cl.19A cell monolayers showed discontinuities in tight junction strands after exposure to mixtures of IFN
and tumour necrosis factor-
(TNF
), which was associated with a preferential increase in macromolecular flux (Rodriguez et al., 1995
). Similar discontinuities have also been reported in the tight junctions of rat proximal tubule (Orci et al., 1981
) and a dual-pathway model has recently been proposed for this tissue involving small tight junction pores (radius 6.7 Å) and infrequent large slit breaks (Guo et al., 2003
), which is similar to that derived for colonocyte monolayers using the PEG profiling approach. Size-selective loosening of endothelial tight junctions has also been reported in claudin-5 deficient mice (Nitta et al., 2003
) although, in contrast to the present studies, the effect appeared to be selective for small molecules of <0.8 kDa. Similarly, the differential effects of TNF
and the phorbol ester TPA on paracellular permeability in LLC-PK1 reported by Mullin and co-workers (Mullin et al., 1997
) are also consistent with size-selective changes in junctional permeability. However, as neither of these studies analysed different size probes simultaneously, it is difficult to determine whether such effects involve activation of different pore populations or simply reflect the degree of dilation of the tight junction as a whole.
As previously hypothesised (Karczewski and Groot, 2000), the presence of multiple size- and charge-limiting pores within the tight junction offers potential for differential regulation of paracellular permeability by tight junction modulators. In previous studies, we have shown that EGTA and sodium caprate (C10) exert profoundly different effects on PEG profiling and sieving parameters in T84 cells (Watson et al., 2001
). Exposure to C10 significantly increased the value of
but did not change restrictive pore radius, suggesting that its primary effect is to increase the number or open probability of restrictive pores. C10 did not significantly influence the non-restrictive pathway as evidenced by a lack of effect on ß. EGTA, on the other hand, dramatically increased restrictive pore radius, abolishing all size discrimination and reverting the paracellular pathway in T84 cells to essentially a one-component system. The current studies demonstrate that the mechanism of action of IFN
, which is to specifically influence the non-restrictive component of the pathway, is quite different and distinct from either of these modulators.
The molecular basis for different populations of paracellular pores and the mechanisms by which IFN modulates the non-restrictive pathway has not been specifically addressed in this study. However, the alterations in two tight junction proteins, occludin and claudin-1, which accompanied the IFN
-mediated changes in permeability, suggest some potential avenues for investigation. Exposure to IFN
markedly reduced the expression of occludin and its association with the detergent-insoluble membrane fraction, a finding that is consistent with other studies (Sugi et al., 2001
; Mankertz et al., 2000
; Oshima et al., 2001
; Bruewer et al., 2003
). Particularly noticeable was the loss of high molecular mass species, which reflects the phosphorylated form of the protein. The phosphorylation state of occludin appears to be an important regulator of paracellular permeability with several studies showing that dephosphorylation coincides with loss of TER and increased solute permeability (Simonovic et al., 2001; Chen et al., 2002
; Ohtake et al., 2003
). In the context of the present study, recent observations in the MDCK kidney epithelial cell line showing that knock down of occludin expression is associated with increases in tight junction permeability to large organic cations with little effect on mannitol permeability are interesting (Yu et al., 2005
).
The increase in claudin-1 found in the detergent-insoluble fraction after IFN treatment is surprising because impairment of the paracellular route has generally been associated with reduced levels of this protein (Kucharzik et al., 2001
; Tedelind et al., 2003
), although recent reports of cytokine-induced increases in claudin-1 expression linked to barrier disruption indicate that this may not always be the case (Han et al., 2003
; Abe et al., 2003
). Interactions between claudins and occludin may be important in determining the properties of tight junction strands (Tsukita and Furuse, 2000
; McCarthy et al., 2000
). Given this, the possibility that an imbalance in claudin-1 and occludin caused by the divergent effects of IFN
on these proteins could be involved in the formation of non-restrictive pores is worthy of consideration. Unlike the effects on occludin, the IFN
-mediated increase in claudin-1 expression did not appear to be fully reversible and it is interesting in this respect that ß values also remained significantly above control levels 96 hours after IFN
washout suggesting that higher levels of the non-restrictive pore may persist after cytokine exposure.
It was important to exclude increased internalisation as an explanation for the selective increases in permeability of larger PEG and dextran molecules (7.4-23Å) induced by IFN. Both PEG and dextran are classical permeability probes being strongly hydrophilic molecules that are unlikely to diffuse across cell membranes to any significant extent, however, there is evidence that dextran, for example, may have a limited capacity to internalise via fluid-phase endocytosis. Shurety and co-workers (Shurety et al., 1998
) demonstrated endocytic uptake of FITC-dextran in MDCK monolayers although the majority of uptake occurred from the basolateral surface with relatively little internalisation from the apical surface. Similarly, in T84, depletion of extracellular calcium has been shown to promote endocytosis of dextran (Ivanov et al., 2004
), a finding which we have confirmed here. However, using the same approach, there was no evidence that IFN
increased dextran internalisation over the small amount seen in untreated monolayers. This, taken together with the 14C-PEG4000 cell-association data, suggests that internalisation and transcellular passage of these paracellular markers plays no significant role in the selective modulation of permeability induced by IFN
.
What is the relevance of these findings to the role of IFN in inflammatory disorders in the gut? Selective activation of a population of large pores within the paracellular pathway by IFN
would allow large antigenic molecules to cross without gross disruption of the epithelial barrier to small molecules, which may occur at a later stage with the establishment of chronic inflammation. Increased exposure of the mucosal immune system to bacterially derived luminal antigens owing to impairment of the epithelial barrier is thought to initiate inflammatory responses (Linskens et al., 2001
). IFN
is increased in inflammatory conditions and has been shown to increase not only the paracellular movement of antigens but also their transcytosis across gut cells (Heyman, 2001
). Selective activation of a population of large pores within the paracellular pathway by IFN
would allow large antigenic molecules to cross without gross disruption of the epithelial barrier to small molecules, which may occur at a later stage with the establishment of chronic inflammation. Mucosal IFN
levels have been shown to increase at an early stage in the development of colitis in mice (Spencer et al., 2002
) and appear to rise very rapidly after oral antigen administration (Kolker et al., 2003
). Although, the PEG profiling technique does not allow us to define the size of the IFN
-stimulated pore, it would be expected to be large enough, based on the marked increase in FD10 permeability, to allow significant translocation of known bacterial antigens with molecular mass in the range 15-25 kDa (Yoshino et al., 1994
; Orwin et al., 2002
). The finding that IFN
markedly increases T84 permeability to E. coli LPS supports this view, as although the precise molecular size of the LPS used here is unknown, purified LPS is thought to be in the region of 10-20 kDa. The routes and mechanisms by which LPS crosses the intestinal epithelium remains a controversial area. LPS can be internalised by gut epithelial cells via a clathrin- and lipid raft-dependent mechanism, a process which is involved in its intracellular recognition by toll-like receptor 4 (Hornef et al., 2003
). However, the significance of this pathway in mediating increased transepithelial passage of endotoxin in disease states is unclear and recent studies (Drewe et al., 2001
; Seth et al., 2004
), including the present one, are consistent with enhanced permeability of LPS via a paracellular route in inflammatory or ischemic conditions. Interestingly, modulation of the tight junction by IFN
does not however appear to be the primary route by which intact bacteria themselves cross the epithelium. Recent evidence from our laboratory has shown that IFN
stimulates internalisation and translocation of commensal bacteria in gut epithelial cells via a raft-mediated transcellular process that appears to be independent of its effects on the paracellular pathway (Clark et al., 2005
). The significance of the low levels of non-restrictive pathway detectable in unstimulated cells is unknown but may indicate a physiological, as well as pathological, role for the pathway. One possibility is that the pathway contributes to the low level sampling of luminal antigens necessary for development of immune tolerance, although there is evidence that this may also occur via a transcytotic route (Shao et al., 2001
).
In conclusion, these studies suggest that proinflammatory cytokines may activate specific pathways within the tight junctions of intestinal epithelial cells that will favour the transepithelial transfer of large antigenic molecules. Although the significance of this pathway in vivo remains to be determined, the implication is that cytokines such as IFN may open up a paracellular route for the entry of luminal antigens without gross disruption of the epithelial barrier to small molecules. Such a pathway could be involved in the early pathogenesis of intestinal inflammatory disorders.
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Acknowledgments |
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