1 Department of Pharmaceutical Chemistry, University of California, 600 16th Street, San Francisco, CA 94143-2280, USA
2 Department of Molecular and Cell Biology, University of California, 142 LSA #3200, Berkeley, CA 94720-3200, USA
* Author for correspondence (e-mail: ccwang{at}cgl.ucsf.edu)
Accepted 4 July 2005
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Summary |
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Key words: CRK, Cell cycle, Cytoskeleton
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Introduction |
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T. brucei exhibits a specific range of cell morphologies defined by the internal cytoskeleton, which is characterized by a precisely arranged subpellicular corset of more than 100 microtubules that are crosslinked to each other and to the plasma membrane (Gull, 1999). These microtubules have their plus (+) ends all pointed toward the posterior end of the cell, consistent with a postulated unified direction of cortical microtubule extension (Robinson et al., 1995
). Each cell possesses a single mitochondrion extending from the anterior to the posterior end of the cell. A mitochondrial DNA complex, termed the kinetoplast, has its own cell cycle coordinated with the nuclear cell cycle, with an S phase and the phase of kinetoplast segregation preceding the nuclear S phase and mitosis, respectively (Ploubidou et al., 1999
; Woodward et al., 1990). A large body of evidence suggests that the kinetoplast cell cycle may not be totally inter-dependent with the nuclear cell cycle, and there exist different molecular mechanisms regulating the kinetoplast and nuclear cell cycles in T. brucei (Das et al., 1994
; Ploubidou et al., 1999
).
Regulatory pathways controlling the eukaryotic cell cycle have been studied in considerable detail in yeast and mammalian cells and shown to involve regulatory proteins such as cyclins and cyclin-dependent protein kinases (CDKs) (Mendenhall and Hodge, 1998). To date, five CDK homologues (designated cdc2-related kinases, CRK1, 2, 3, 4 and 6), four PHO80 homologues and three B-cyclin homologues have been identified in the T. brucei genome (Hammarton et al., 2003a
). By RNA interference (RNAi) experiments, CRK3 and CycB2/CYC6 were found to control the G2-M checkpoint transition, whereas CRK1 and a Pho80 homologue CycE1/CYC2 were found to play important roles in the G1-S passage in both the procyclic and bloodstream forms of T. brucei (Hammarton et al., 2003b
; Hammarton et al., 2004
; Li and Wang, 2003
; Tu and Wang, 2004
). The procyclic form, when arrested in G1 phase by a double knockdown of CRK1 and CRK2, had an unusual morphology with an elongated and occasionally branched posterior end extended by newly synthesized microtubules (Hammarton et al., 2004
; Tu and Wang, 2005
). These findings suggested that posterior morphogenesis is coupled with G1-S passage in the procyclic form, and that CRK2 may regulate the cell cycle-associated changes in the cytoskeleton.
To further understand the mechanism behind formation of the elongated/branched posterior end, the latter was examined by transmission electron microscopy and found to consist of the expected corset microtubule extension plus an elongated/branched mitochondrion. However, this morphological aberration was not observed in the bloodstream-form cells arrested in G1 phase, which, unlike the procyclic form, requires a triple knockdown of CRK1+CRK2+CycE1/CYC2 to stop 15% of the cells from DNA synthesis completely. When the latter were differentiated in vitro under G1 arrest, the procyclic form thus produced had an apparently normal morphology of a procyclic form even though it was under G1 arrest as its bloodstream-form precursor. The data thus revealed a clear distinction in cell cycle regulation and cytoskeletal modulation between the two life stages of T. brucei.
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Materials and Methods |
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The bloodstream form T. brucei strain 90-13 (Wirtz et al., 1999) was cultivated at 37°C in HMI9 medium supplemented with 10% fetal bovine serum and 10% serum plus (JRH Biosciences) (Hirumi and Hirumi, 1989
). G418 (2.5 µg/ml), hygromycin B (5 µg/ml) and phleomycin (2.5 µg/ml) were also added to stabilize the plasmids in the cell.
Transmission electron microscopy
Cells were fixed in 2% glutaraldehyde, 4% paraformaldehyde in 0.1 M cacodylate buffer, pH 7.2, for 1 hour at room temperature and post-fixed in 1% osmium tetroxide, 0.8% potassium ferricyanide, 5 mM calcium chloride in 0.1 M cacodylate buffer, pH 7.2, for 45 minutes. Cells were spun onto poly-L-lysine-coated Aclar® tabs, dehydrated with ethanol, infiltrated with Epon and processed in a similar manner as previously described (Muller-Riechert et al., 2003). Briefly, cells attached to Aclar® tabs were flat-embedded on top of coated glass slides, and cured at 60°C for 2 days. Aclar® tabs were removed and cells of interest were identified using phase microscopy, marked using a diamond knife, cut out and remounted for sectioning. Ultrathin sections (50-65 nm) were collected and stained with uranyl acetate and lead citrate and imaged in a JEOL 1200 transmission electron microscope.
Mitotracker staining
CRK1+CRK2-deficient procyclic-form cells were induced by 1 µg/ml tetracycline for 5 days. Cells were harvested and suspended in fresh culture medium at a density of 1x106-1x107 cells/ml. MitotrackerTM Green FM (Molecular Probe) was dissolved in dimethyl sulfoxide at 1 mM and added to a final concentration of 5 µM. The mixture was incubated for 20 minutes at 26°C, centrifuged, washed with fresh culture medium and incubated for another 20 minutes. Cell samples were collected, washed twice with phosphate-buffered saline (PBS; 137 mM NaCl, 8 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, pH 7.4) and fixed in 4% paraformaldehyde at 4°C for 15 minutes. Slides were mounted in Vectashield (Vector Laboratories, Burlingame, CA) in the presence of 1 µg/ml 4,6-diamino-2-phenylindole (DAPI) and examined with an Olympus phase-contrast and fluorescence microscope.
Rhizoxin treatment
The CRK1+CRK2-deficient procyclic-form cells were treated with rhizoxin at a final concentration of 1 nM and incubated for 5 days. Samples were collected and stained with the antibody YL1/2 (Chemicon, rat monoclonal antibodies against yeast tyrosinated -tubulin, 1:400 dilution) or MitotrackerTM Green FM as described previously (Tu and Wang, 2005
). Slides were mounted in Vectashield in the presence of DAPI and examined with a fluorescence microscope.
RNA interference (RNAi)
Partial cDNA fragments (250-550 bp) of CRK1, CRK2, CRK4 and CRK6 (Trypanosome Genome Database accession numbers X64314, X74598, AJ413200 and AJ505556) were amplified by PCR using pairs of gene-specific primers (Tu and Wang, 2005), and ligated pair-wise. Another partial fragment of CycE1/CYC2 (accession number AJ242519) nucleotides 26-420 was also amplified and ligated with CRK1 and CRK2 (sequences available upon request). All these combinations were cloned into the pZJM vector by replacing its
-tubulin fragment (Wang et al., 2000
). The resulting RNAi constructs were linearized with NotI for integration into the T. brucei rDNA spacer region.
The fragment of a unique sequence from the coding region of each gene that has no significant sequence identity among the rest of the genome sequences in the Trypanosome Genome Database was ligated into three pairs (CRK1+CRK2, CRK1+CRK4, CRK1+CRK6) and a triplet (CycE1/CYC2+CRK1+CRK2) and subcloned into the RNAi vector pZJM (Tu and Wang, 2005; Wang et al., 2000
). The newly generated sequences around the junctions of ligation in each combination were also examined in the genome database, and there was no significant sequence identity found among the rest of the genome sequences. It is thus unlikely that, by using these DNA constructs in RNAi experiments, expression of an unidentified gene could be inadvertently knocked down.
Transfection of the bloodstream-form T. brucei by electroporation was performed as previously described (Tu and Wang, 2004). The transfectants were selected with the addition of 2.5 µg/ml phleomycin and cloned on 0.6% agarose plates (Carruthers and Cross, 1992
). The stable transfectants thus selected were grown in culture medium containing phleomycin. Transcription of the DNA insert was induced by adding 1 µg/ml tetracycline to the medium to switch on the T7 promoter. The effects of depleting multiple mRNAs on the growth of bloodstream-form trypanosome cells were monitored by a daily counting of the number of transfected cells using a hemocytometer.
Semi-quantitative RT-PCR
Total RNA was extracted from T. brucei cells using the TRIzol reagent (Amersham Pharmacia). Before the PCR reaction, DNase I was added to the total RNA extract and incubated at 37°C for 30 minutes to remove the remaining DNA. A 100-500 ng total RNA sample was added to an RT-PCR using the one-step RT-PCR kit (Invitrogen) and a pair of gene-specific primers that differ from the primer pair used in generating the original RNAi construct (sequences available upon request). -Tubulin mRNA (TUB) was also amplified as a sampling control. The reaction mixture was first incubated at 50°C for 30 minutes and then at 95°C for 2 minutes. The PCR cycle was maintained at 95°C for 30 seconds, 55°C for 45 seconds and 72°C for 45 seconds for a total of 30 cycles.
Fluorescence-activated cell sorting (FACS) analysis
Cell samples for FACS analysis were prepared as described previously (Tu and Wang, 2004; Tu and Wang, 2005
). The DNA content of propidium iodide-stained cells was analyzed with a FACScan analytical flow cytometer using the CELLQuest software (Becton Dickinson). Percentages of cells in each phase of the cell cycle, G1, S and G2-M, were determined by the ModFitLT V3.1 software (Becton Dickinson). The same propidium iodide-stained cell samples were also examined with a fluorescence microscope for tabulation of cells containing different numbers of nuclei and kinetoplasts from a population of about 200 cells.
BrdU incorporation
5-Bromo-2-deoxyuridine (BrdU) was added to the T. brucei cells at a concentration of 0.3 mM 3 days after RNAi induction and the cells were harvested after a further 2 days of incubation. Immunofluorescence assays for the incorporated BrdU were then performed as described previously (Tu and Wang, 2004; Tu and Wang, 2005
). Mouse anti-BrdU monoclonal antibody and fluorescein isothiocyanate (FITC)-conjugated anti-mouse immunoglobulin G (IgG)-stained slides were mounted in Vectashield in the presence of DAPI and examined with a fluorescence microscope.
In vitro differentiation
RNAi was induced in the transfected bloodstream-form T. brucei cells by tetracycline for 3 days at 37°C. Temperature of the cell culture was then reduced to 26°C, and 5 mM sodium citrate and 5 mM sodium cis-aconitate were added to initiate the process of differentiation (Czichos et al., 1986). At time intervals of 0, 12, 24, 36, 48 and 60 hours, cells were harvested and lysed in lysis buffer (10 mM Tris, 25 mM KCl, 10 mM NaCl, 5 mM MgCl2 and 0.2 mM EDTA, pH 7.6). The lysate, fractionated by SDS-PAGE, was blotted onto nitrocellulose membrane and stained with antibodies against VSG 221 (from G.A.M. Cross, Rockefeller University; rabbit polyclonal antibodies against the variant surface glycoprotein VSG221; diluted 1:10,000) and antibody against procyclin (Accurate Chemical and Scientific Corporation; mouse monoclonal antibody against procyclin; diluted 1:1000). Western blotting of the time samples during differentiation was used to monitor the VSG221 disappearance and the emergence of procyclin as indicators of the progression of differentiation (Li et al., 2003
; Mutomba and Wang, 1998
). The same blot was also stained with the antibody to
-tubulin (Sigma; mouse monoclonal antibody against
-tubulin, diluted 1:1000) for sample loading control. Horseradish peroxidase (HRP)-conjugated donkey antiserum against rabbit immunoglobulin G (IgG) (Amersham Pharmacia, diluted 1:1000) and HRP-conjugated goat antiserum against mouse IgG (Amersham Pharmacia, diluted 1:1000) were used as secondary antibodies.
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Results |
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In the bloodstream form of T. brucei a knockdown of CRK1 and CRK2 only enriched cells in the G1 phase without inhibiting nuclear DNA synthesis
To verify if the unusual coupling between posterior morphogenesis and G1-S transition observed in the procyclic form also applies to the bloodstream form of T. brucei, we tried first to use the RNAi technique to arrest the latter effectively in the G1 phase. Our previous effort showed that a knockdown of CRK1 in the bloodstream form reduced cell growth to 17% of the control with an increase of G1-phase cells from 45% to 60% and a corresponding decrease of S-phase cells from 43% to 28% without appreciable change in the G2-M population (Tu and Wang, 2004). Those data suggested that a slowing down of G1-S passage resulted from CRK1 depletion and an important role played by CRK1 in regulating this passage in the bloodstream form. For a more complete cell arrest in the G1 phase, we tried RNAi pair-wise knockdowns including CRK1 and another CRK2, 4 or 6 in the bloodstream form. CRK3 was not included, because it is known to have a specific function in regulating only the G2-M transition (Tu and Wang, 2004
).
Experimental results indicated that a knockdown of CRK1+CRK2 in the bloodstream form led to more significant growth arrest than if CRK1 was knocked down alone (Fig. 4A). The number of double knockdown cells reached less than 6% of that in the uninduced control after 4 days of RNAi. The generation time was calculated to increase from 7.5 hours in the control to 11 hours. This enhanced growth arrest by the combined knockdowns may suggest that CRK1 and CRK2 play a redundant role in regulating the G1-S passage. For CRK1+CRK4 and CRK1+CRK6 double knockdowns, a growth inhibition similar to that of CRK1 knockdown alone was observed (data not shown), which agrees with the finding from our previous studies that CRK4 and CRK6 do not play any apparent role in cell cycle regulation in T. brucei (Tu and Wang, 2004).
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Propidium iodide-stained cells were examined for those containing one nucleus-one kinetoplast (1N1K), one nucleus-two kinetoplasts (1N2K), two nuclei-two kinetoplasts (2N2K), no nucleus-one kinetoplast (0N1K, the zoid) and multiple nuclei-multiple kinetoplasts (XNXK). The CRK1+CRK2 knockdown resulted in a 10% increase in 1N1K cells from 78% to 88% with a corresponding 4% decrease in 1N2K and 6% decrease in 2N2K cells without detectable emergence of either zoids or XNXK cells (Fig. 5A).
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The 74% of G1 cells estimated by FACScan and the 88% of 1N1K cells by fluorescence microscopy 4 days following CRK1+CRK2 knockdown suggested a majority of cells arrested in the G1 phase. However, data from BrdU incorporation from day 3 to day 5 after RNAi initiation indicated that essentially all the 1N1K cells are still capable of synthesizing DNA (Fig. 5B). The G1 cells are thus not truly arrested in this phase but, rather, progressing through the G1-S checkpoint at a slower rate. This is quite different from that observed in the procyclic form of T. brucei, in which a CRK1+CRK2 knockdown led to 50% of the population arrested in G1 phase incapable of DNA synthesis (Tu and Wang, 2005). These discrepant data suggest that there are distinctive molecular mechanisms regulating cell cycle progression in the two developmental stages of T. brucei.
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A CycE1/CYC2, CRK1 and CRK2 triple knockdown fails to show any morphological change in the bloodstream form
As a previous knockdown of the expression of CycE1/CYC2 from the procyclic form of T. brucei resulted in G1 arrest accompanied with an elongated posterior end (Li and Wang, 2003), we tested the same knockdown in the bloodstream form and noticed only a partial enrichment of G1 cells without any indication of inhibited DNA synthesis or morphological change (Z. Li and C.C.W., unpublished). In a further attempt to arrest the bloodstream-form cells in the G1 phase, we performed a triple knockdown of CycE1/CYC2, CRK1 and CRK2 by RNAi. The number of triple knockdown cells was only 2% of that in the uninduced control after 4 days of RNAi induction. The generation time of these triple knockdown cells was increased from 7.5 hours in the control to 13 hours (Fig. 7A), which is longer than the 11-hour generation time for the CRK1+CRK2 double knockdown cells (Fig. 4A). The number of G1 cells increased from 45% to 75%, S-phase cells decreased from 38% to 16% whereas the number of G2-M-phase cells reduced from 14% to 6% (Fig. 7B). The data are consistent with those from the karyotype distribution study which showed a 74% to 90% increase of 1N1K cells accompanied by a corresponding 14% to 8% decrease in 1N2K and a reduction from 12% to 2% in 2N2K cells (Fig. 7C). There was no zoids or XNXK cells detectable in the triple knockdown population.
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Differentiation of bloodstream-form T. brucei arrested in G1 phase resulted in procyclic-form cells with normal morphology
The distinctive morphologies between the bloodstream and procyclic forms of T. brucei trapped in the G1 phase prompted us to look into the in vitro differentiation of G1-arrested bloodstream form into the procyclic form with the intention of examining the morphology of the latter. It is known that the differentiation of T. brucei from bloodstream form into procyclic form is not coupled with cell cycle progression (Li et al., 2003). It can proceed with the cells arrested in any phase of the cell cycle (Li et al., 2003
). The control, the CRK1+CRK2 double knockdown and the CycE1/CYC2+CRK1+CRK2 triple knockdown bloodstream-form cells were subjected to in vitro differentiation by an established procedure (see Materials and Methods). Progression of differentiation was monitored by the disappearance of variant surface glycoprotein VSG221 and the emergence of procyclins on the cell membrane surface by western analysis of the timed cell samples (Li et al., 2003
; Mutomba and Wang, 1998
). The process of VSG221 shedding and procyclin appearance was completed within 60 hours for the control, the double knockdown and the triple knockdown cells (Fig. 8A). There was, however, a slower shedding of VSG221 by about 12 hours and a delayed appearance of procyclin by some 12-24 hours in the knockdown cells, which could result from the knockdowns. As the process of differentiation was essentially completed in all cases, it is unclear whether the somewhat lengthened time course in the mutants could have any effect on the eventual outcome. An immunofluorescence examination of the procyclic-form cells thus derived indicated that the double and triple knockdown cells maintained an apparently normal morphology like the wild-type procyclic form cells (Fig. 8B). There was no YL1/2 staining of the posterior ends in these cells, whereas a CRK1+CRK2 depleted procyclic form demonstrated an elongated/branched posterior end filled with newly synthesized microtubules (Fig. 8B). A BrdU incorporation study showed that after differentiation, most of the CRK1+CRK2-deficient cells were still as capable of synthesizing their nuclear DNA as the control cells, but
15% of the CycE1/CYC2+CRK1+CRK2-depleted procyclic-form cells (based on a total count of 600 cells from three independent experiments) were incapable of incorporating BrdU (Fig. 8C). Thus, the procyclic form retains the same state of partial G1 arrest as the bloodstream form from which it is derived. It also retains a normal procyclic-form morphology, even though the same double (Tu and Wang, 2005
) and triple knockdowns (our unpublished data) in the procyclic form are known to cause aberrant morphology and much more severe restriction of cells in the G1 phase.
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Discussion |
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As knockdown of CRK1 expression alone in procyclic-form T. brucei did not lead to elongated/branched posterior ends in cells (Tu and Wang, 2004), CRK2 was considered as the potential kinase playing a pivotal role in posterior morphogenesis (Tu and Wang, 2005
). This is not unlikely, as a Cdc2 homologue is associated with the cortical microtubules in protoplasts of carrot, tobacco and Arabidopsis cells (Hemsley et al., 2001
) and many other protein kinases have been implicated in mediating cytoskeletal modulations such as the p21-activated kinase family (Cau et al., 2001
; Marcus et al., 1995
). However, as knockdown of CRK2 alone did not result in an elongated posterior end in the procyclic form either, it seems probable that the posterior extension is a consequence of G1 arrest, rather than a direct result of CRK2 loss.
Distinct cytoskeletal modulation between two life cycle forms of T. brucei
The cytoskeletal modulation in procyclic-form T. brucei apparently does not apply to the bloodstream form of T. brucei. When CRK1 and CRK2 were depleted simultaneously in the latter, the cells have an apparently normal morphology without an extended/branched posterior end. As our previous knockdown of CycE1/CYC2 from the procyclic form resulted in cells with elongated posterior ends (Li and Wang, 2003), we performed a triple knockdown of CycE1/CYC2+CRK1+CRK2 in the bloodstream form with 90% of the cells in the G1 phase, but it still resulted in cells with normal morphology.
One significant morphological difference between the two forms of T. brucei lies in the position of the kinetoplast. In the procyclic form, the kinetoplast is located midway between the nucleus and the posterior end, whereas in the bloodstream form, the kinetoplast is located at the extreme posterior end of the cell. Differentiation of the bloodstream form into the procyclic form requires the repositioning of the kinetoplast to a location midway between the nucleus and cell posterior (Matthews et al., 1995). This repositioning may require cytoskeletal changes involving the extension of microtubule corset toward the posterior end of the cell (Hendriks et al., 2001
). The requirement for this cytoskeletal modulation reflects the need for one of the two nuclei to migrate between the two segregated kinetoplasts prior to cytokinesis in the procyclic form (Sherwin and Gull, 1989
; Woodward and Gull, 1990
). But such a migration of the nucleus is apparently not required prior to cytokinesis in the bloodstream form (Hammarton et al., 2003a
). Thus, a posterior extension may become an essential step in the procyclic form during the G1 phase to reposition the kinetoplast, which is apparently ended by the action of CRK2 during the G1-S transition (Tu and Wang, 2005
). But a similar event does not occur in the bloodstream form. Thus a knockdown of CRK2 expression in the latter does not result in an elongated/branched posterior end.
However, this explanation is challenged when the CRK1+CRK2 double knockdown and CRK1+CRK2+CycE1/CYC2 triple knockdown bloodstream form cells differentiated into the procyclic form without showing any elongated/branched posterior ends or any other abnormal morphology. A careful examination of the positions of kinetoplasts in these newly formed procyclic-form cells indicated that they have moved midway between the nuclei and the posterior ends as seen in typical procyclic form cells (Fig. 8B and supplementary material Fig. S3). This migration of kinetoplast together with the loss of VSG221 and emergence of procyclin indicate normal progression of differentiation while the cells are under G1 arrest. But it is not necessarily an indication of completion of the differentiation process. Although not coupled to cell cycle progression, this process is believed to represent a change in the profile of gene expression when the environmental temperature drops from 37°C to 26°C (Diehl et al., 2002). The change is attributed primarily to a post-transcriptional alteration of mRNA stability involving specific regions in the 3'-untranslated regions of the mRNA and the corresponding binding proteins (Blattner and Clayton, 1995
), which may occur throughout the G1, S, G2 as well as M phases in the cell cycle. The arrest of cells in G1 phase may prevent the completion of a total transformation from bloodstream to procyclic form, because the necessary post-transcriptional events that occur only in the cell cycle phases other than G1 are not accomplished. Thus, the procyclic-form cells derived from differentiation under G1 arrest may have acquired only part of the procyclic characteristics, which may explain their similarities to the bloodstream form in terms of nuclear DNA synthesis and a lack of morphological change.
Overexpression of a differentiation-associated CCCH zinc finger protein TbZFP2 in the bloodstream form leads to an elongated posterior morphology in the differentiated procyclic-form cells (Hendriks et al., 2001). It is likely that TbZFP2 is involved in the posterior extension of the microtubule corset, which probably repositions the kinetoplast during differentiation. A knockdown of TbZFP2 expression would thus predict a failure in kinetoplast repositioning during differentiation resulting in a `procyclic form' that retains some of the bloodstream characteristics.
Different G1-S checkpoint regulations between procyclic and bloodstream forms of T. brucei
Another distinction between the two forms of T. brucei is reflected in the apparently more complex mechanisms of regulating G1-S passage in the bloodstream form. Although a CRK1+CRK2 knockdown resulted in an 80% population in G1 phase and 50% of the population incapable of nuclear DNA synthesis in the procyclic form (Tu and Wang, 2005), the same knockdown led to a 74% population in G1 phase with essentially all the cells still capable of nuclear DNA synthesis in the bloodstream form. Even in the CRK1+CRK2+CycE1/CYC2 triple knockdown with 90% of the bloodstream-form cells retained in G1 phase, only 15% of the cells were incapable of incorporating BrdU. This remarkable ability of continued nuclear DNA synthesis indicates continued passage from G1 into S phase despite depletion of the cyclin and CRKs known to control G1-S transition in T. brucei (Li and Wang, 2003
; Tu and Wang, 2004
; Tu and Wang, 2005
). More proteins may be involved in controlling G1-S transition in the bloodstream form that remain to be identified.
In summary, we observed that the elongated and/or branched posterior ends in CRK1+CRK2-deficient procyclic-form cells are filled with microtubules accompanied with mitochondrial structure. This morphological aberration is, however, not observed in the bloodstream form or the procyclic form derived from differentiation of the bloodstream form depleted of CRK1+CRK2 or CRK1+CRK2+CycE1/CYC2. The regulation of G1-S transition has thus an apparently much more complex mechanism in the bloodstream form.
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Acknowledgments |
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Footnotes |
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References |
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Blattner, J. and Clayton, C. E. (1995). The 3'-untranslated regions from the Trypanosoma brucei phosphoglycerate kinase-encoding genes mediate developmental regulation. Gene 162, 153-156.[CrossRef][Medline]
Carruthers, V. B. and Cross, G. A. (1992). High-efficiency clonal growth of bloodstream- and insect-form Trypanosoma brucei on agarose plates. Proc. Natl. Acad. Sci. USA 89, 8818-8821.
Cau, J., Faure, S., Comps, M., Delsert, C. and Morin, N. (2001). A novel p21-activated kinase binds the actin and microtubule networks and induces microtubule stabilization. J. Cell Biol. 155, 1029-1042.
Czichos, J., Nonnengaesser, C. and Overath, P. (1986). Trypanosoma brucei: cis-aconitate and temperature reduction as triggers of synchronous transformation of bloodstream to procyclic trypomastigotes in vitro. Exp. Parasitol. 62, 283-291.[CrossRef][Medline]
Das, A., Gale, M., Jr, Carter, V. and Parsons, M. (1994). The protein phosphatase inhibitor okadaic acid induces defects in cytokinesis and organellar genome segregation in Trypanosoma brucei. J. Cell Sci. 107, 3477-3483.
Diehl, S., Diehl, F., El-Sayed, N. M., Clayton, C. and Hoheisel, J. D. (2002). Analysis of stage-specific gene expression in the bloodstream and the procyclic form of Trypanosoma brucei using a genomic DNA-microarray. Mol. Biochem. Parasitol. 23, 115-123.[CrossRef]
Gull, K. (1999). The cytoskeleton of trypanosomatid parasites. Annu. Rev. Microbiol. 53, 629-655.[CrossRef][Medline]
Hammarton, T. C., Mottram, J. C. and Doerig, C. D. (2003a). The cell cycle of parasitic protozoa: potential for chemotherapeutic exploitation. Prog. Cell Cycle Res. 5, 91-101.[Medline]
Hammarton, T. C., Clark, J., Douglas, F., Boshart, M. and Mottram, J. C. (2003b). Stage-specific differences in cell cycle control in Trypanosoma brucei revealed by RNA interference of a mitotic cyclin. J. Biol. Chem. 278, 22877-22886.
Hammarton, T. C., Engstler, M. and Mottram, J. C. (2004). The Trypanosoma brucei cyclin, CYC2, is required for cell cycle progression through G1 phase and for maintenance of procyclic form cell morphology. J. Biol. Chem. 279, 24757-24764.
Hemsley, R., McCutcheon, S., Doonan, J. and Lloyd, C. (2001). P34cdc2 kinase is associated with cortical microtubules from higher plant protoplasts. FEBS Lett. 508, 157-161.[CrossRef][Medline]
Hendriks, E. F., Robinson, D. R., Hinkins, M. and Matthews, K. R. (2001). A novel CCCH protein which modulates differentiation of Trypanosoma brucei to its procyclic form. EMBO J. 20, 6700-6711.
Hirumi, H. and Hirumi, K. (1989). Continuous cultivation of Trypanosoma brucei blood stream forms in a medium containing a low concentration of serum protein without feeder cell layers. J. Parasitol. 75, 985-989.[Medline]
Kilmartin, J. V., Wright, B. and Milstein, C. (1982). Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J. Cell Biol. 93, 576-582.[Abstract]
Li, Y., Li, Z. Y. and Wang, C. C. (2003). Differentiation of Trypanosoma brucei may be stage non-specific and does not require progression of cell cycle. Mol. Microbiol. 49, 251-265.[CrossRef][Medline]
Li, Z. and Wang, C. C. (2003). A PHO80-like cyclin and a B-type cyclin control the cell cycle of the procyclic form of Trypanosoma brucei. J. Biol. Chem. 278, 20652-20658.
Marcus, S., Polverino, A., Chang, E., Robbins, D., Cobb, M. H. and Wigler, M. H. (1995). Shk1, a homolog of the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe. Proc. Natl. Acad. Sci. USA 92, 6180-6184.
Matthews, K. R., Sherwin, T. and Gull, K. (1995). Mitochondrial genome repositioning during the differentiation of the African trypanosome between life cycle forms is microtubule mediated. J. Cell Sci. 108, 2231-2239.
Mendenhall, M. D. and Hodge, A. E. (1998). Regulation of Cdc28 cyclin-dependent protein kinase activity during the cell cycle of the yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62, 1191-1243.
Muller-Riechert, T., Hohenberg, H., OToole, E. T. and McDonald, K. (2003). Cryoimmobilization and three-dimensional visualization of C. elegans ultrastructure. J. Microsc. 212, 71-80.[CrossRef][Medline]
Mutomba, M. C. and Wang, C. C. (1998). The role of proteolysis during differentiation of Trypanosoma brucei from the bloodstream to the procyclic form. Mol. Biochem. Parasitol. 93, 11-22.[CrossRef][Medline]
Ploubidou, A., Robinson, D. R., Docherty, R. C., Ogbadoyi, E. O. and Gull, K. (1999). Evidence for novel cell cycle checkpoint in trypanosomes: kinetoplast segregation and cytokinesis in the absence of mitosis. J. Cell Sci. 112, 4641-4650.
Robinson, D. R., Sherwin, T., Ploubidou, A., Byard, E. H. and Gull, K. (1995). Microtubule polarity and dynamics in the control of organelle positioning, segregation, and cytokinesis in the trypanosome cell cycle. J. Cell Biol. 128, 1163-1172.[Abstract]
Sherwin, T. and Gull, K. (1989). The cell division cycle of Trypanosoma brucei brucei: timing of event marker and cytoskeletal modulations. Philos. Trans. R. Soc. Lond. 323, 573-588.[Medline]
Sherwin, T., Schneider, A., Sasse, R., Seebeck, T. and Gull, K. (1987). Distinct localization and cell cycle dependence of COOH terminally tyrosinolated -tubulin in the microtubules of Trypanosoma brucei brucei. J. Cell Biol. 104, 439-446.[Abstract]
Tu, X. M. and Wang, C. C. (2004). The involvement of two cdc2-related kinases (CRKs) in Trypanosoma brucei cell cycle regulation and the distinctive stage-specific phenotypes caused by CRK3 depletion. J. Biol. Chem. 279, 20519-20528.
Tu, X. M. and Wang, C. C. (2005). Coupling of posterior cytoskeletal morphogenesis to the G1/S transition in the Trypanosoma brucei cell cycle. Mol. Biol. Cell 16, 97-105.
Vassella, E., Straesser, K. and Boshart, M. (1997). A mitochondrion-specific dye for multicolour fluorescent imaging of Trypanosoma brucei. Mol. Biochem. Parasitol. 90, 381-385.[CrossRef][Medline]
Vickerman, K. (1985). Developmental cycles and biology of pathogenic trypanosomes. Br. Med. Bull. 41, 105-114.[Medline]
Wang, Z., Morris, J. C., Drew, M. E. and Englund, P. T. (2000). Inhibition of Trypanosoma brucei gene expression by RNA interference using an integratable vector with opposing T7 promoters. J. Biol. Chem. 275, 40174-40179.
Wehland, J., Willingham, M. C. and Sandoval, I. V. (1983). A rat monoclonal antibody reacting specifically with the tyrosylated form of -tubulin. I. Biochemical characterization, effects on microtubule polymerization in vitro, and microtubule polymerization and organization in vivo. J. Cell Biol. 97, 1467-1475.[Abstract]
Wirtz, E., Leal, S., Ochatt, C. and Cross, G. A. (1999). A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei. Mol. Biochem. Parasitol. 99, 89-101.[CrossRef][Medline]
Woodward, R. and Gull, K. (1990). Timing of nuclear and kinetoplast DNA replication and early morphological events in the cell cycle of Trypanosoma brucei. J. Cell Sci. 95, 49-57.[Abstract]
Yaffe, M. P., Harata, D., Verde, F., Eddison, M., Toda, T. and Nurse, P. (1996). Microtubules mediate mitochondrial distribution in fission yeast. Proc. Natl. Acad. Sci. USA 93, 11664-11668.
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