Wales Heart Research Institute, Department of Cardiology, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK
* Author for correspondence (e-mail: blayney{at}cf.ac.uk)
Accepted 13 July 2005
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Summary |
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Key words: Ryanodine receptor, FKBP12, Surface plasmon resonance, Channel activation state
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Introduction |
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RyR open probability in bilayers shows a biphasic response to Ca2+, being closed at <0.01 µM Ca2+ or 1 mM Ca2+, and the greatest open probability being at 1-10 µM Ca2+ (Meissner et al., 1988). ATP enhances the sensitivity to Ca2+. The pharmacological modulators, caffeine and ruthenium red, open and close the channel, respectively (Meissner et al., 1988
). In situ, RyR1 can be activated by allosteric interaction with the dihydropyridine receptor (DHPR), which acts as a voltage sensor (Numa et al., 1990
), or by trigger Ca2+ propagated laterally as Ca2+ is released via the RyR1 itself a process termed calcium induced calcium release (CICR) (Fabiato and Fabiato, 1975
). In cardiac muscle, CICR is the prime activation mechanism with the trigger Ca2+ originating extracellularly through the cardiac DHPR subtype acting as an L-type Ca2+ channel (Williams, 1997
). Several mechanisms have been proposed for closure of RyRs (Fill, 2003
) including inhibitory feedback by mM Ca2+ (Wang et al., 2004
).
RyR is regulated by accessory proteins, including FK506 binding protein 12 (FKBP12) (Chelu et al., 2004), a member of a protein family characterised by the binding of the immunosuppressant drugs FK506 and rapamycin (Kay, 1996
). FKBP12 association with RyR1 was revealed by their persistent co-purification (Collins, 1991
; Jayaraman et al., 1992
). The RyR/FKBP stoichiometry is one FKBP per RyR subunit (Jayaraman et al., 1992
; Timerman et al., 1995
) and topological analysis revealed four FKBP12 molecules bound symmetrically proximal to the N-terminus (Samso and Wagenknecht, 1998
). FKBP12 binding modifies RyR function by stabilising subunit interactions, eliminating subconductance states and decreasing the open probability of the channel (Ahern et al., 1997
; Brillantes et al., 1994
; Chen et al., 1994
; Ma et al., 1995
). In bilayer studies, RyR1 or RyR2 channels opened and closed together on addition of FKBP12/12.6 a process termed coupled gating (Marx et al., 1998
; Marx et al., 2001
).
Dissociation of FKBP12/12.6 from RyR1/RyR2 is proposed as a common mechanism for the dysfunction of both skeletal (Reiken et al., 2003; Ward et al., 2003
) and cardiac muscle in heart failure (Wehrens et al., 2004
). Point mutations in RyR1 causing central core disease and/or malignant hyperthermia (Dirksen and Avila, 2002
) or in RyR2 associated with cardiac sudden death syndromes have been reported (Wehrens and Marks, 2003
), and the latter show altered interaction with FKBP12.6. However, the role of RyR/FKBP interaction in both pathologies is disputed (George et al., 2003
; Jiang et al., 2002
; Stange et al., 2003
; Xiao et al., 2005
; Xiao et al., 2004
). Thus, defining the role of FKBP12/RyR interaction in EC coupling in both normal physiology and pathology, and understanding the mechanism of interaction in normal and mutant RyR, are of fundamental importance.
Here, we have scrutinised the inter-relationship of RyR1 and FKBP12 with respect to the open and closed state of the RyR channel per se. We used surface plasmon resonance (SPR) and recombinant GST-FKBP12 coupled to a Biacore sensor chip. Equilibrium kinetics was measured for native RyR1 with physiological and pharmacological modulators of channel open and closed states. [3H]Ryanodine binding confirmed the open or closed status of the channel in each condition. The equilibrium binding rate and affinity constants segregated dependent on the open and closed state. The major determinant of change was a decreased rate of dissociation of FKBP12 from RyR1 when the channel was closed.
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Materials and Methods |
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Quantity of RyR protein
RyR1 was analysed on a SDS PAGE gel (5.5%) with a protein standard curve of BSA 10 µg to 200 µg/ml (20 µl per lane) (Laemmli, 1970), stained with Coomassie (Biorad Biosafe) and RyR protein content determined from the BSA standard curve by densitometry using Biorad Quantity One software.
Preparation and purification of GST-FKBP
FKBP12 was amplified from a cardiac cDNA library, cloned into a pGEX vector and expressed and purified according to manufacturer's protocol (Amersham Biosciences) (Blayney et al., 2004). Purified GST-FKBP12 was stored at 80°C.
[3H]Ryanodine binding assays
Ryanodine binding was determined using 1 µg of RyR1 protein per assay incubated with 5 nM [3H]ryanodine (100 Ci/mmol, Amersham) for 90 minutes at 37°C (Lai et al., 1989). Binding buffer contained 50 mM HEPES (pH 7.4), 25 mM Tris, 500 mM KCl, pH 7.4, with either 1 mM EGTA (<0.01 µM Ca2+), 1 µM Ca2+ (final free) ± 1 mM ATP, 1 mM Ca2+, 1 µM ruthenium red or 10 mM caffeine. Free Ca2+ concentration was varied using admixtures of 1 mM EGTA and 1 mM Ca2+.
ELISA binding assays
RyR1 was coated onto ELISA plates and blocked with basic buffer (20 mM PIPES, 0.05% CHAPS, 150 mM NaCl, 1% BSA, pH 7.1) for 1 hour (Blayney et al., 2004). GST-FKPB12 was added in basic buffer, which included 1 mM EGTA, final free Ca2+ concentrations between 0.1 and 1000 µM, ± 1 mM ATP, caffeine or ruthenium red and incubated for 2 hours at 22°C. Bound GST-FKBP12 was detected using a rabbit anti-GST Ab (Blayney et al., 2004
). In a control experiment, 1 µM FK506 was included in the GST-FKBP12 binding step.
Surface plasmon resonance studies
Anti-GST Ab was covalently coupled to a CM3 chip (GST coupling kit, Biacore). Recombinant GST-FKBP12 or GST (control) were bound (ligand) to the anti-GST Ab by adjusting the time of contact to achieve 100 resonance units (RU), using binding wizard software (Biacore 3000). RyR1 preparations (analyte) were flowed over the chip surface in binding buffer (20 mM PIPES, 0.05% CHAPS, 150 mM NaCl, pH 7.1), at protein concentrations up to 250 µg/ml, flow rate 30 µl/minute, using the kinetic inject mode. Some experiments included 1 µM FK506 a specific inhibitor of FKBP12 or recombinant FKBP12 (Sigma) was pre-incubated with RyR1 for 30 minutes. Additions to the binding buffer included 1 mM EGTA, 1 µM free Ca2+ ± 1 mM ATP, 1 µM ruthenium red or 10 mM caffeine. BIA Evaluation software calculated the KA, ka and kd using the base line drift correction algorithm. Experiments were repeated four times using RyR1 preparations from different rabbits.
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Results |
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[3H]Ryanodine binding
Ryanodine binds to the open conformation of RyR and is considered a reliable measure of the open/closed state (Lai et al., 1989). For SPR experiments (see below) 1 mM EGTA, 1 µM free Ca2+ ± 1 mM ATP, 1 mM Ca2+, 10 mM caffeine and 1 µM ruthenium red were the chosen assay conditions based on the results of the ELISA assays (Fig. 1B,C). The results in Fig. 1D, normalised by dividing by the value for 1 mM EGTA, show that binding was 30-60-fold higher in the presence of 1 µM Ca2+ ± 1 mM ATP and 10 mM caffeine than in the presence of 1 mM EGTA, 1 mM Ca2+ or 1 µM ruthenium red compared with the caffeine value, Fig. 1D. Thus, 1 µM Ca2+ ± 1 mM ATP and caffeine opened the RyR1 channel, and it was closed in the presence of 1 mM EGTA and 1 mM Ca2+.
Surface plasmon resonance
Fig. 2A illustrates a typical series of sensorgrams for the addition of serial RyR1 concentrations using the kinetic inject wizard. Fig. 2B compares the binding of RyR1 to GST-FKBP or GST bound to the chip and shows that there was no binding to the GST moiety. Fig. 2C shows that total binding was incremental with RyR1 protein concentration and was inhibited by 1 µM FK506, a specific inhibitor of FKBP12/protein interactions. Fig. 2D shows that binding to GST-FKBP12 could be inhibited by pre-binding recombinant FKBP12 to the RyR1, confirming that the FKBP moiety of GST-FKBP interacted with the correct binding site on RyR1. The molar ratio of FKBP12:RyR1 in the analyte, expressed as a function of the percentage of maximum binding (without FKBP), showed that 50% inhibition was obtained at a molar ratio of 5:1.
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The dependence of binding on RyR1 analyte concentration was determined (Fig. 4). Total RU units bound (for each sensorgram) was normalised to the total RU units of GST-FKBP12 on the chip and plotted against the RyR protein concentration added. Scatter plots show that binding was linearly related to added RyR1 protein (Fig. 4A,B). The amount bound was greater per unit of added protein for conditions where the channel was closed, compared with open. Fig. 4C,D shows the same data averaged for each experiment and the four individual experiments statistically compared. Fig. 4C shows that for the physiological agents (1 µM Ca2+ ±1 mM ATP) that open the channel, binding was reduced compared with 1 mM EGTA and 1 mM Ca2+ (channel closed). In the presence of the pharmacological agent caffeine (channel open), RyR showed significantly less binding to FKBP12 than in the presence of ruthenium red (channel closed).
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Discussion |
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ELISA binding assays to determine the relationship between FKBP12 and the open/closed status of RyR1 showed that RyR1 bound less FKBP12 at Ca2+ concentrations that are associated with activation (opening) of RyR1 that is, 1 µM and 10 µM Ca2+ (enhanced by the addition of 1 mM ATP) compared with RyR inhibition with EGTA (<0.01 µM Ca2+) and 1 mM Ca2+. Ruthenium red increased the binding of FKBP12, relative to that of the unmodulated control, whereas caffeine had no effect, suggesting that the RyR1 preparation, stripped of endogenous FKBP12, was in the open state. [3H]Ryanodine binding assays confirmed the open and closed state of the RyR1 and were appropriately modulated in agreement with previous single channel analysis (Meissner et al., 1988).
SPR was used to determine equilibrium binding kinetics. To establish specificity of binding, GST was bound to the chip and used to confirm that no binding was detected to this moiety alone (Fig. 2B). FK506 effectively abolished binding over a range of RyR1 concentrations (Fig. 2C). Endogenous FKBP12 was removed from RyR1 during preparation with rapamycin and was shown by western blot to be within subsaturation levels. Loss of FKBP12 was confirmed by pre-mixing recombinant FKBP12 with RyR1, before flow over coupled GST-FKBP12, and binding was inhibited (Fig. 2D). Inhibition of 50% was obtained at a molar ratio of 5:1. This is close to the stoichiometric ratio of 4:1 (one binding site per subunit of RyR1) and was within the appropriate range of concentrations for the interaction of the two molecules (Timerman et al., 1995
). This result also confirmed that GST-FKBP12 bound to the same site as free FKBP.
The Biacore method is based on binding signal (RU) being proportional to the mass of protein bound. Thus, for 100 RU of GST-FKBP12 (38 kDa), the equivalent binding signal for RyR (2200 kDa) is maximally 5800 RU, although 500 RU was observed. Binding was, however, linearly related to the RyR1 protein concentration added (Fig. 2A,C and Fig. 4A,B). Being small molecules, GST-FKBP12 and the anti-GST Ab can bind within the dextran matrix of the CM3 chip, which is inaccessible to the large RyR1 molecule, resulting in a limited proportion of the total FKBP12 accessible on the surface. Moreover, binding one 2200 kDa RyR1 oligomer to GST-FKBP12 probably obscures neighbouring unliganded GST-FKBP12 molecules.
The KA for the closed state of the RyR was 1x1013/14 M1, which is 4-5 orders of magnitude higher than for the open channel (
1x109 M1). KA is derived from ka/kd, and the increased affinity of closed RyR1 for FKBP12 was due to a decreased kd. Nevertheless, the open channel affinity was comparable to an Ab-antigen interaction so, although the open channel is more vulnerable to dissociation of FKBP12, this loss would be slow. The KA equates to KD values of
0.1-0.01 pM for the closed channel compared with
1 nM for the open channel. A previous study derived a value of
300 nM for KD (Timerman et al., 1995
); the slow rate of dissociation may have contributed to this lower affinity because a labelled FKBP12 exchange experiment was used. In a previous study, modulators of RyR1 open and closed states had no effect on the quantity of FKBP12 bound to RyR1 in GST-FKBP12 pulldown assays (Bultynck et al., 2001
), thus our observations reflect the discriminatory power of the SPR technique. The impact of complete loss of FKBP12/12.6 from RyR1/RyR2 are changes in the stability of the open/closed state of RyR in bilayers (Ahern et al., 1997
; Brillantes et al., 1994
; Chen et al., 1994
; Ma et al., 1995
) and altered EC coupling and Ca2+ sparks in skeletal (Avila et al., 2003
) and cardiac (Gomez et al., 2004
; Prestle et al., 2001
; Xiao et al., 1997
) myocytes. Our data suggests that, because FKBP12 dissociates slowly and retains a comparatively high affinity for the open channel, loss of FKBP12 from RyR may not be a part of the normal EC coupling cycle. With regard to the pathology of heart failure, where both RyR1 (Reiken et al., 2003
; Ward et al., 2003
) and RyR2 (Wehrens et al., 2004
) are reported to be stripped of FKBP12/12.6 by `hyperphosphorylation', the latter would need to have effects over and above increasing the open state of the channel (Meissner, 2004
).
The RyR1 tetramer undergoes a considerable symmetrical conformational change between the open and closed states (Serysheva et al., 1999). It is not known whether the functional open and closed states equate to the same physical conformational changes for all agents for example, a pharmacological agent could block the channel pore without physically altering the protein structure. Here, we show that KA segregated the channel modulators according to their effect on the open or closed states. This suggests that both the physiological and pharmacological agents studied here broadly affect the RyR in equivalent ways, altering the conformation of the protein and manifesting as a change in the dissociation rate constant, kd, for FKPB12.
In this study, we have shown that FKBP12 equilibrium binding kinetics are modified by the open or closed state of RyR1. The affinity is greatest for the closed RyR1 channel and, although four to five orders of magnitude lower for the open conformation, the interaction remains at high affinity for both channel states. The underlying cause of the change in affinity is likely to be a conformational change in the RyR affecting the FKBP12 binding site (Zissimopoulos and Lai, 2005). In the future, it will be interesting to observe whether modulations such as phosphorylation, or mutations such as those causing malignant hyperthermia, change the RyR1/FKBP12 interaction, differently from the modulation of the open/closed conformation. In conclusion, these studies show that SPR provides a highly sensitive technique to discern changes in the interaction of modulatory proteins with RyR1 that reflect its activation state.
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Acknowledgments |
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