Department of Genetics, University of Pennsylvania School of Medicine, 415 Curie Blvd, Philadelphia, PA 19104-6145, USA
Author for correspondence (e-mail: calvi{at}mail.med.upenn.edu)
Accepted 9 June 2005
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Summary |
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Key words: DNA replication, Double parked, Cdt1, Geminin, Genome stability
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Introduction |
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In recent years, there have been significant insights into the mechanisms that restrict pre-RC assembly to once per cell cycle. CDK activity is required for the initiation of DNA replication, but CDKs also prevent reassembly of the pre-RC during S, G2 and early M phases (Broek et al., 1991; Dahmann et al., 1995
). In the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, CDKs phosphorylate ORC, CDC6 and MCMs, resulting in their inactivation, degradation and exclusion from the nucleus (Drury et al., 2000
; Jallepalli et al., 1997
; Labib et al., 1999
; Nguyen et al., 2000
; Nguyen et al., 2001
; Tanaka and Diffley, 2002
; Vas et al., 2001
). In multicellular animals (metazoa), it appears that CDKs also prevent re-replication, perhaps by regulating multiple pre-RC subunits, although the mechanism is less well-defined than it is in yeast (Ballabeni et al., 2004
) (reviewed in Bell and Dutta, 2002
; Coverley et al., 2000
; Ekholm-Reed et al., 2004
; Hua et al., 1997
; Itzhaki et al., 1997
). There is another inhibitor of rereplication that is unique to metazoa, Geminin (Gem), which is expressed during S, G2 and early M phases. (McGarry and Kirschner, 1998
; Tada et al., 2001
; Wohlschlegel et al., 2000
). Gem binds directly to Cdt1 to inhibit its ability to load the MCM complex onto origins (Lee et al., 2004
; Saxena et al., 2004
; Tada et al., 2001
; Wohlschlegel et al., 2000
). Through several CDK targets and Gem inhibition, it appears that the cell has taken a multipronged approach to preventing the catastrophic mistake of unbalanced genomic replication.
Although multiple pre-RC subunits are targeted by CDKs, the requirement for the Gem inhibitor, and other recent evidence, suggests that regulation of Cdt1 is especially important to prevent re-replication in metazoa (for review, see Saxena and Dutta, 2005). Mutation or inactivation of Gem can result in at least partial re-replication suggesting that it plays an important role to restrain Cdt1 (Melixetian et al., 2004
; Mihaylov et al., 2002
; Quinn et al., 2001
; Tada et al., 2001
; Vaziri et al., 2003
; Zhu et al., 2004
). Cdt1 is also rapidly destroyed at the onset of S phase, and several ubiquitin-ligase complexes have been implicated in targeting Cdt1 for proteasome degradation (Hu et al., 2004
; Li and Blow, 2005
; Li et al., 2003
; Liu et al., 2004
) (for a review, see Saxena and Dutta, 2005
; Sugimoto et al., 2004
; Zhong et al., 2003
). This destruction is crucially important because increased levels of Cdt1 are sufficient to induce re-replication and cell death in a range of organisms, despite the presence of Gem (Castellano Mdel et al., 2004
; Gopalakrishnan et al., 2001
; Li and Blow, 2005
; Maiorano et al., 2005
; Nishitani et al., 2004
) (for reviews, see Saxena and Dutta, 2005
; Thomer et al., 2004
; Vaziri et al., 2003
). Cdt1 also appears to be the target of a checkpoint response to genotoxic damage and is rapidly degraded in G1 phase in response to ultraviolet light or ionizing irradiation via a ubiquitin-mediated pathway (Higa et al., 2003
; Hu et al., 2004
; Kondo et al., 2004
). Cdt1 expression is elevated in several human cancers and, in mice, overexpression of Cdt1 in erythroid cells increases their oncogenic potential (Arentson et al., 2002
; Karakaidos et al., 2004
; Xouri et al., 2004
). The emerging picture is that tight regulation of Cdt1 is crucial for protecting genome integrity. It remains unclear, however, what coordinates the rapid destruction of Cdt1 with the onset of S phase.
We have previously shown that the D. melanogaster ortholog of Cdt1, Double-parked (Dup) (Whittaker et al., 2000), is rapidly destroyed beginning at the G1-S transition and that this degradation is important because elevated levels of Dup induced profound re-replication and cell death during development (Thomer et al., 2004
). We also showed that Dup is a direct target of cyclin-E/CDK2. Although inhibition of cyclin-E/CDK2 completely blocked Dup degradation, mutation of the phosphorylation sites within Dup did not block its degradation at the onset of S phase. This suggested that other CDK2-dependent mechanisms ensure degradation of Dup in S phase, independent of direct phosphorylation by CDK2. Here, we present evidence that it is DNA replication itself that is the CDK2-dependent mechanism that triggers Dup degradation. Defects in different steps of DNA replication result in stabilization of Dup protein in developing tissues, with Dup accumulating to highest levels in stem cells. Replication stress also results in a concomitant increase in Gem protein in cells with high levels of Dup. We propose a model wherein Dup destruction is coupled to DNA replication, and that when problems with DNA replication are encountered, Gem balances Dup accumulation to prevent origin relicensing.
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Materials and Methods |
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Immunolabeling and microscopy
Antibody and BrdU labeling were performed as previously described (Calvi and Lilly, 2004; Schwed et al., 2002
; Thomer et al., 2004
). In most cases, we used an affinity-purified guinea-pig antibody against Dup (1:1000) provided by Terry Orr-Weaver, Whitehead Institute, Cambridge, MA (Whittaker et al., 2000
). Results were confirmed with an affinity-purified rabbit polyclonal anti-Dup antibody (1:500) kindly provided by Eileen Beall and Michael Botchan (University of California, Berkeley, CA). Anti-Gem antibody was raised and purified as described (Thomer et al., 2004
) and used at 1:500. Other antibodies and their concentrations were as follows: anti-cyclin-E monoclonal 8B10 (1:5) (Richardson et al., 1995
); anti-cyclin-B monoclonal (1:4) (University of Iowa Hybridoma Bank, Iowa City, IA) (Lehner and O'Farrell, 1990
); anti-phosphorylated histone H3 (1:500; Upstate Biotechnology) (Hendzel et al., 1997
). Alexa 488 and Alexa 568 (Molecular Probes) or Cy3 (Jackson ImmunoResearch) secondary antibodies (1:400) of appropriate specificity were used for detection. Dup immunofluorescence was quantified using a Leica SP Confocal and TCS-NT quantification software, and by using Openlab quantification software on non-confocal images. All images used for quantification were matched for exposure between wild-type and mutant or drug-treated, and not saturated for pixel intensity.
RNA in-situ hybridization
For in-situ hybridization probe, a dup cDNA was isolated by reverse-transcription PCR (RT-PCR) and subcloned into the pCR2.1-Topo plasmid (Invitrogen). This template was used to generate antisense or sense (control) digoxigenin-labeled RNA probes by in-vitro transcription (Roche). The probes were used for in-situ hybridization according to standard methods (Tautz and Pfeifle, 1989). Third-instar wild-type and Mcm63 larval brains were processed in parallel in three separate experiments.
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Results |
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Interestingly, the accumulation of Dup protein was cell-type specific. Highest levels of Dup were consistently seen in neuroblast stem cells (NB) of the brain and ventral ganglion, which were identified by their large size, position and absence of labeling for prospero protein (Fig. 1B and data not shown) (Ceron et al., 2001; Ito and Hotta, 1992
; Vaessin et al., 1991
). Dup was also increased, but to a much lesser extent, in the surrounding NB daughter cells and some cells of the optic-lobe proliferation centers (Fig. 1B). An increase in Dup levels was observed as early as day 2 of development in brains of Mcm63 mutant first-instar larvae when replication defects are first manifest because of depletion of maternal Mcm6 protein stores (data not shown). This suggested that replication defects in Mcm63 result in increased levels of Dup.
Dup protein persists into G2 and M phase
If the increase in Dup levels is due to a failure to degrade Dup during S phase, one prediction is that Dup should persist past the G1-S transition, when it is normally rapidly destroyed. To address this, we used antibodies against cyclin B, which labels cells from late S phase until metaphase, and against phosphorylated histone H3 (PH3), which labels condensed chromosomes in mitosis (Hendzel et al., 1997; Lehner and O'Farrell, 1990
). Many cells with increased Dup also labeled for cyclin B (74%, n=54) (Fig. 1D). Many of these cells had cyclin B restricted to the cytoplasm, which is indicative of G2 phase, whereas others had cyclin B in cytoplasm and nucleus, suggesting that they had entered M phase. This also showed that some cells had high levels of Dup restricted to the nucleus, whereas, in others, Dup was distributed throughout the cell, which was confirmed by labeling nuclear envelope for lamin C and cell membranes for
-spectrin (Fig. 1D and data not shown). Many of the Mcm63 cells with high levels of Dup also labeled with PH3 (30%, n=27) (Fig. 1F). The morphology and number of these mitotic chromosomes was highly abnormal, suggesting that genome integrity was compromised in these cells (Fig. 1F and data not shown). This suggests that some Mcm63 mutant cells do not permanently maintain a checkpoint arrest and enter mitosis despite severe defects in DNA replication.
Unexpectedly, all wild-type stem cells also had very low, but detectable, levels of Dup protein in G2 and M phase (Fig. 1C,E). This labeling in G2 and M phase was not observed in the primary stem-cell daughter [ganglion mother cell (GMC)] or cells that arise from subsequent divisions of the GMC, despite the fact that these stem cells and many of their daughters are actively dividing at this developmental time (Fig. 1C,E). This suggests that the mechanisms controlling Dup steady-state levels differ somewhat in stem cells. Similar results for wild-type and Mcm6 mutant stem cells were obtained with an independently raised Dup antibody (E. Beall and M. Botchan), which did not label dup mutant cells, confirming that the labeling represents Dup (data not shown). These results suggest that Dup persists and accumulates past the G1-S transition in Mcm63 mutant cells, and that this effect is enhanced in stem cells.
Replication defects increase Dup protein primarily by a post-transcriptional mechanism
The persistence of Dup protein past the G1-S transition suggested that its normal degradation does not occur when DNA replication is defective. We previously showed that degradation of Dup is robust; overexpression of a Myc-epitope-tagged Dup from a strong hsp70 promoter did not result in detectable Dup protein during S phase (Thomer et al., 2004). It remained possible, however, that an increase in Dup transcription during replication stress could contribute to the observed rise in protein levels. To examine this, we performed in-situ hybridization to dup mRNA in wild-type and Mcm63 mutant brains. In the wild type, dup mRNA was most abundant in stem cells of the mid-brain and ventral ganglion, and in dividing lamina precursor cells in the optic lobe (Fig. 2A). In Mcm63 mutant brains, dup mRNA abundance was not increased but was instead clearly less abundant than in wild-type brains in three separate experiments in which hybridization was performed in parallel (Fig. 2B).
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Accumulation of Dup is not specific to defects in Mcm6
Given that Dup is required to load the MCM complex into the pre-RC, one hypothesis was that the failure to degrade Dup could represent a feedback mechanism that senses defects in origin licensing. Alternatively, Dup degradation might be sensitive to other defects in DNA replication. To address this, we fed Drosophila larvae the replication inhibitor HU, which causes replication-fork arrest. After 27 hours on medium containing HU, S phase was inhibited in brain cells, as evidenced by greatly reduced BrdU incorporation (data not shown). HU also resulted in greatly increased levels of Dup and, like Mcm63 cells, stem cells often had higher levels of Dup than their surrounding daughter cells (Fig. 2E, Fig. 3A, and data not shown). Typically 50-100 cells in the brain lobes and ventral nerve cord (VNC) had levels of Dup that were two to three times the wild-type levels (n=20 brains), although the intensity of labeling and number of cells were variable among different brains, presumably owing to differences in HU delivery. HU also increased Dup in some diploid cells of imaginal discs and polyploid cells of the gut, but to a lesser extent than that seen for stem cells of the brain (data not shown). Cyclin-B labeling of brains from HU-treated animals indicated that 100% cells with abnormally high levels of Dup also labeled with cyclin B, indicating that HU results in Dup persisting past the G1-S transition (data not shown). Similar results were obtained when origin licensing or DNA-polymerase processivity was impaired in mutants for Orc2 (orc21) and PCNA (mus20902448), respectively (data not shown) (Landis et al., 1997
; Spradling et al., 1999
). Because only a subset of cells in the brain had increased Dup levels, we were unable to detect an increase in Dup abundance by western blotting of whole-brain lysates (data not shown). These results indicate that a failure to degrade Dup is not specific to mutation of Mcm6 but that Dup is also stabilized when other steps in DNA replication are defective.
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Stabilization of Dup is not sensitive to ATM/ATR activity
Stabilization of Dup during replication stress raised the possibility that this could be the result of a replication checkpoint. To test this idea, we examined Dup levels in checkpoint-compromised animals. The Drosophila mei-41 gene resembles the ATR (ATM- and RAD3-related) kinase from humans and mediates the DNA-replication and -damage checkpoint in flies (Boyd et al., 1976; Brodsky et al., 2000
; Hari et al., 1995
; Sekelsky et al., 2000
; Sibon et al., 1999
). Flies homozygous for the null allele mei-4129D are viable but sensitive to HU and other mutagens owing to a defective checkpoint response (Brodsky et al., 2000
). To determine whether mei-41 signaling is required for the increase of Dup, we examined brains from mei-4129D homozygotes fed HU. Labeling for PH3 indicated that many mei41-mutant brain cells proceeded into mitosis despite inhibition of DNA replication by HU, consistent with an impaired checkpoint in these cells. These mutants also had an enhanced abnormal mitotic chromosome phenotype after HU treatment (Fig. 3D). Nonetheless,
50-100 cells in mei-4129D brains still displayed intense Dup labeling after HU treatment (n>20 brains), comparable in intensity and number of cells to wild-type animals fed HU in parallel (Fig. 3A,B). These results indicate that the ATR homolog mei41 is not required for the accumulation of Dup protein.
The Drosophila tefu gene resembles ATM from human cells and is required for the maintenance of telomere structure and the checkpoint response to double-strand DNA breaks (Bi et al., 2004; Oikemus et al., 2004
; Sekelsky et al., 2000
; Silva et al., 2004
; Song et al., 2004
). It remained possible, therefore, that tefu mediates the stabilization of Dup in response to DNA damage incurred during replication stress. To address this and to eliminate the possibility of partial redundancy between mei-41 and tefu, we inhibited both kinases by feeding larvae the ATM/ATR kinase inhibitor caffeine (Boyd and Setlow, 1976
; Sarkaria et al., 1999
). Like mei-41 animals, the brain cells of larvae fed caffeine for 27 hours followed by HU and caffeine for an additional 27 hours proceeded to metaphase at a higher frequency and had more severe chromosomal abnormalities than those fed HU alone (data not shown). Despite this evidence for an impaired checkpoint, the increases in Dup levels in response to HU were similar with or without caffeine (Fig. 3C). Similar results were obtained when mei-4129D animals were fed caffeine and HU (data not shown). It is possible, however, that even these caffeine-treated animals retain a low level of ATM/ATR activity. Nonetheless, the large increase in Dup levels suggests that the canonical genotoxic stress checkpoints might not be required to stabilize Dup in response to replication stress.
Various replication defects alter Dup abundance and localization in follicle cells
One possible cause for the accumulation of Dup protein is that cells arrest in a cell-cycle phase during which Dup degradation does not occur. This seemed unlikely given that Dup levels were increased in both wild-type and checkpoint-defective cells that failed to maintain cell-cycle arrest. To examine this question further, and to gain insight into the requirements for Dup degradation, we turned to the follicle cells of the ovary. During stage 10B of oogenesis, these terminally differentiated cells enter a final extended S phase during which only a few origins repeatedly initiate, resulting in the developmental amplification of eggshell protein and other genes (Calvi et al., 1998; Claycomb et al., 2004
; Spradling and Mahowald, 1980
). The activity of these amplification origins can be visualized as distinct subnuclear foci by BrdU or fluorescent in-situ hybridization during stages 10B-13 of oogenesis (Calvi et al., 1998
; Calvi and Spradling, 2001
). Antibodies against several replication proteins also label the amplified regions as distinct foci (Fig. 4A,B) (for review, see Tower, 2004
).
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To gain further insight into the requirements for normal Dup regulation, we analysed other hypomorphic mutants that impair amplification in the ovary. All these mutants also increased Dup levels in amplification-stage follicle cells, although the increase was, in some cases, not as great as that seen for Mcm6K1214 (Fig. 4E-H). The product of the mus101 gene is similar to Dpb11 in S. cerevisiae and TopBP1 in humans, and acts downstream of pre-RC assembly for the initiation and elongation phases of replication, and also participates in a checkpoint response (Araki et al., 1995; Makiniemi et al., 2001
; Wang and Elledge, 1999
; Yamamoto et al., 2000
). Dup staining was delocalized and increased in follicle cells homozygous for the hypomorphic amplification mutant allele mus101K451 (Fig. 4E) (Komitopoulou et al., 1983
). Dup protein was also delocalized and increased in chiffon0233 mutants (Fig. 4F) (Calvi et al., 1998
). The chiffon gene is similar to DBF4, the activating subunit of the essential S-phase kinase CDC7, whose targets include the MCM proteins (Landis and Tower, 1999
) (for a review, see Sclafani, 2000
). Finally, mutation of two members of the pre-RC increased the levels of Dup: Orc2293 and dupPA77, a mis-sense mutant of dup itself (Fig. 4G,H) (Royzman et al., 1999
; Whittaker et al., 2000
). Together with the results from mutant brain cells, the data suggest that the regulation of Dup stability is sensitive to several steps in DNA replication. These steps range from activation by kinases upstream of initiation to the polymerase processivity factor PCNA at the replication fork.
Gem levels are increased during replication stress
We previously showed that an increase in Dup levels is sufficient to induce re-replication in Drosophila, and similar results have been obtained for other Cdt1 family members in a range of organisms (Arias and Walter, 2004; Castellano Mdel et al., 2004
; Li and Blow, 2004
; Maiorano et al., 2005
; Thomer et al., 2004
; Vaziri et al., 2003
). The increase in Dup levels in response to replication stress therefore represents a vulnerable condition for the cell during which inappropriate origin relicensing may occur. However, Gem, the inhibitor of Dup, is present from S phase until mid-M phase and could potentially restrain relicensing when defects in Dup degradation are encountered (McGarry and Kirschner, 1998
; Mihaylov et al., 2002
; Nishitani et al., 2001
; Quinn et al., 2001
; Tada et al., 2001
; Wohlschlegel et al., 2000
). Our previous results had indicated, however, that normal levels of Gem are insufficient to prevent relicensing when Dup is only mildly overexpressed from a transgene (Thomer et al., 2004
).
To investigate the levels of Gem protein in replication-stressed cells, we used antibodies that we raised against Gem to label normal and replication-stressed brain cells (Thomer et al., 2004). In normal brain and disc cells, double labeling with antibodies against cell-cycle markers indicated that Gem was abundant in the nucleus during S, G2 and early M phase, similar to previous reports in Drosophila and other organisms (Fig. 5A and data not shown) (Quinn et al., 2001
) (for review, see Saxena and Dutta, 2005
). This included the neuroblast stem cells in the mid-brain, suggesting that Gem might be important to balance the low level of Dup protein that is present in these cells during G2 (Fig. 5A,B).
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Discussion |
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Destruction of Dup is coupled to DNA replication
We had previously shown that Dup degradation required cyclin-E/CDK2 and that Dup is a direct substrate of this kinase. However, mutation of the phosphorylation sites in Dup only partially stabilized it and this phosphorylation-site mutant of Dup was still rapidly degraded as cells entered S phase. We therefore suggested that CDK2 might regulate Dup stability in two ways: directly by phosphorylation and indirectly through another CDK2-dependent mechanism in S phase. Here, we find that cells of the brain and ovary have high levels of Dup protein when DNA replication is defective. This was not due to increased transcription and did not depend on the canonical checkpoint pathways mediated by ATM/ATR kinases. Instead, we propose that impaired degradation of Dup, combined with continued translation from a low level of persistent mRNA, leads over time to accumulation of high levels of Dup during S and G2 phase (Fig. 6C). Importantly, this suggests that, in the unstressed cell, the destruction of Dup during S phase is coupled to ongoing DNA replication (Fig. 6B). Because DNA replication depends on CDK2 activity, this provides a mechanism by which this kinase can regulate Dup stability independently of direct phosphorylation. We found that defects in different steps of DNA replication elicited an increase in Dup levels, from impaired pre-RC activation by the CDC7/Dbf4 kinase (chiffon mutants) to inhibition of replication fork elongation in PCNA mutants (mus101 mutants) or upon HU treatment (Fig. 6A). The triggering of Dup destruction by replication itself would be an efficient way for the cell to ensure that origin relicensing does not occur during S phase.
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While this manuscript was in preparation, Arias and Walter reported results from a Xenopus in-vitro replication system that are very similar to our results in Drosophila (Arias and Walter, 2004). They showed that depletion of several different replication proteins from embryo extracts coordinately impaired DNA synthesis and the ubiquitination and destruction of Cdt1. They found that steps as far downstream as polymerase-
, which synthesizes the DNA primer, were required for normal Cdt1 degradation, and here we extend that to the downstream step of loading PCNA onto those primers. Similar to Dup in flies, they also found that this stabilization of Xenopus Cdt1 in response to replication stress was independent of ATM/ATR-checkpoint-kinase activity and provided evidence that Cdt1 degradation in Xenopus is not solely regulated by direct phosphorylation by CDK2.
The dependence of Cdt1 degradation on replication is reminiscent of previous observations from Xenopus, in which ubiquitination and degradation of the p27-like inhibitor protein Xic1 at origins by the SCF ligase also depends on initiation of DNA replication but is independent of cyclin-E/CDK2 phosphorylation of Xic1 (Furstenthal et al., 2001; You et al., 2002
). It is also reminiscent of SCF-mediated degradation of human Orc1 in S phase, which might be independent of its direct phosphorylation by CDK2 (Mendez et al., 2002
). Thus, the mechanism that links the destruction of Dup/Cdt1 to DNA replication might target other proteins for destruction to promote S-phase progression and prevent re-replication. The similarity of our findings to those in vertebrates further suggests that the mechanism for Cdt1 degradation is conserved in multicellular eukaryotes.
Developmental modulation of Dup cell-cycle expression
Our previous results suggested that, in most cells, Dup is abundant in the nucleus in late G1 phase and disappears rapidly upon S-phase entry. Unexpectedly, we found that, in the absence of replication stress, stem cells and lamina-precursor cells in the brain have a low but detectable level of Dup during G2 and M phase. Stem cells clearly had the highest levels of Dup during replication stress, even compared with their immediate daughter cells. In the absence of stress, in-situ hybridization showed that Dup mRNA is most abundant in stem cells and lamina-precursor cells, and that some mRNA persisted into S and G2 phase, similar to the cell-cycle distribution of human Cdt1 mRNA (Fig. 2A and data not shown) (Nishitani et al., 2001). In the context of our model, the relatively high levels of Dup in stem cells during replication stress are caused by a defect in protein degradation combined with continued translation from a relatively higher steady-state level of Dup mRNA. It is possible, however, that other aspects of Dup regulation differ in stem cells. Interestingly, unchallenged germ-line stem cells also had high levels of Dup relative to other cells (data not shown). Investigation into Dup regulation in stem cells might provide clues to how their cell cycles differ to support their function in tissue homeostasis.
Despite the presence of Dup during S and G2 phase in normally cycling stem cells, there is no evidence that they undergo re-replication. We found that Gem protein is present in these cells during S, G2, and M phase, which probably prevents origin relicensing. An unexpected implication of our observations is that Gem might be more important in some cells than in others during development to protect genome integrity.
Dup, Gem and genome integrity
Evidence from a range of organisms indicates that Cdt1 must be tightly regulated by proteolysis and Gem to prevent re-replication. The high levels of Dup in the replication-stressed cell therefore represents a vulnerable condition when origin re-licensing could occur. We found, however, that the Dup inhibitor Gem was also elevated during replication stress. This suggests that Gem might play a prominent role to prevent re-replication when Dup degradation is impaired during replication stress (Fig. 6C). We could not directly test this model here because replication was acutely blocked by the same defects that led to elevated Dup and Gem. In a cell with mild defects and transiently arrested in the checkpoint, however, Gem might be crucial to prevent origin relicensing so that, when cells eventually resume normal cycling, they duplicate their genome only once. This fail-safe role for Gem during the checkpoint is consistent with previously suggested models (for a review, see Saxena and Dutta, 2005) (Tada et al., 2001
; Wohlschlegel et al., 2000
). It was reported previously that overexpression of Cdt1 in human cells results in a concomitant rise in Gem levels, suggesting there might be a conserved mechanism that actively maintains the proper balance of these two proteins (Vaziri et al., 2003
). Given that CDKs might play other roles in preventing re-replication, inhibition of CDKs during a checkpoint arrest, together with elevated levels of Cdt1, puts the cell especially at risk for inappropriate origin re-licensing. This has important medical implications because elevated Cdt1 protein is found in several human cancers, is sufficient to induce re-replication in human cells and increases the oncogenic potential of mouse erythroid cells (Arentson et al., 2002
; Karakaidos et al., 2004
; Melixetian et al., 2004
; Vaziri et al., 2003
; Xouri et al., 2004
; Zhu et al., 2004
). It is therefore possible that, under certain conditions, such as replication stress, defects in Cdt1 degradation might contribute to genome instability and transformation of the cancer cell.
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Acknowledgments |
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Footnotes |
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References |
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