Department of Molecular Biology, Schultz Laboratory, Princeton University, Princeton, NJ 08544, USA
* Author for correspondence (e-mail: jschwarzbauer{at}molbio.princeton.edu)
Accepted 4 July 2005
![]() |
Summary |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Key words: Extracellular matrix, Integrins, Fibronectin, Matrix assembly, Fibroblasts
![]() |
Introduction |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Heterodimeric integrin receptors and intracellular factors play important roles throughout assembly. Fibril formation is mediated primarily by 5ß1 integrin binding to the RGD cell binding sequence and the adjacent synergy site in the cell binding domain of fibronectin (Fogerty et al., 1990
; McDonald et al., 1987
; Sechler et al., 1997
; Sechler et al., 1996
). Other integrins including
vß3,
IIbß3 and
4ß1 can mediate matrix assembly when appropriately activated (Sechler et al., 2000
; Wennerberg et al., 1996
; Wu et al., 1996
; Wu et al., 1995
) and
vß1 has been shown to temporarily replace
5ß1 during embryogenesis of
5-null mice (Yang and Hynes, 1996
). On the cytoplasmic side, connections from integrins to the actin cytoskeleton directly affect the fibronectin matrix assembly process (Hynes, 1990
; Wu et al., 1995
). In addition, signaling proteins like focal adhesion kinase (FAK), Src and Rho GTPase regulate assembly (Ilic et al., 2004
; Wierzbicka-Patynowski and Schwarzbauer, 2002
; Zhang et al., 1994
).
Within the 3D environment of tissues, cells are affected by signals from the ECM and neighboring cells. Furthermore, cell responses to 3D matrices differ from cells grown on 2D surfaces (Cukierman et al., 2001; Sechler et al., 1998
; Tamariz and Grinnell, 2002
; Vlodavsky et al., 1980
; Wenk et al., 2000
). To test the requirements for fibronectin assembly in three dimensions, we followed fibril formation by cells grown on a cell-free 3D fibronectin matrix. We found that
5ß1-mediated matrix assembly was stimulated in cells on this 3D matrix. The rate of accumulation of detergent-insoluble matrix was increased, the threshold concentration of fibronectin needed for fibril formation was reduced, and the stimulatory effect was dependent on the 3D fibrillar architecture. Furthermore, tumor cells expressing
5ß1 integrins with reduced activity and CHO cells expressing activation-dependent
vß3 were also stimulated to assemble fibronectin fibrils. Our results show that a 3D ECM network contains regulatory cues that enhance fibronectin matrix assembly.
![]() |
Materials and Methods |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Preparation of 3D fibronectin matrix
The procedure for preparing cell-free 3D fibronectin matrix is modified from a published protocol (Chen et al., 1978). Briefly, NIH3T3 or WI-38 (VA13) cells were cultured in wells of a 24-well plate until highly confluent. Cells were washed twice with 1 ml PBS followed by two washes with 1 ml of wash buffer I (100 mM Na2HPO4, pH 9.6, 2 mM MgCl2, 2 mM EGTA). 1 ml lysis buffer (8 mM Na2HPO4, pH 9.6, 1% NP-40) was added to each well and incubated at 37°C for 15 minutes; this was then removed and replaced with 1 ml fresh lysis buffer and incubation was continued for 40-60 minutes. Matrices were washed twice with 1 ml wash buffer II (300 mM KCl, 10 mM Na2HPO4, pH 7.5) and four times with 1 ml dH2O. Matrix could be stored in PBS at 4°C for a few weeks. To examine matrix by confocal microscopy, human 3D fibronectin matrices on glass coverslips were fixed and immunostained with anti-human fibronectin antibody (HFN7.1) and fluorescein-conjugated goat anti-mouse IgG. Images were collected with a Ziess 510 confocal microscope. For SDS-PAGE, a confluent cell monolayer and a 3D matrix were solubilized in parallel with 2x SDS sample buffer (100 mM Tris-HCl, pH 6.8, 30% glycerol, 4 mM EDTA, 6% SDS, 0.01% Bromophenol Blue, 10% ß-mercaptoethanol). Equal proportions of the two samples were electrophoresed on a 6% polyacrylamide gel containing SDS and proteins were visualized by silver staining.
2D substrates were prepared by coating wells with a 10 µg/ml solution of rat or human plasma fibronectin in PBS at 4°C overnight at the indicated concentrations. Total amounts of fibronectin in 3D and 2D substrates were comparable as determined by immunoblotting of solubilized substrate proteins (data not shown).
Analysis of matrix assembly by deoxycholate (DOC)-lysis
For experiments with human cells (WI-38, dermal fibroblasts), the 3D fibronectin matrix was prepared from NIH3T3 cell cultures. For all other cell types, WI-38 (VA13) cell cultures were used. 3D fibronectin matrix and 2D-coated substrates were blocked with 1% BSA in PBS at room temperature for 1 hour. Subconfluent cells were trypsinized, washed and 2x105 cells were seeded per well in DMEM supplemented with 2% fibronectin-depleted FBS. For cells that make limited amounts of their own fibronectin (CHO and HT1080), 2.5x105 cells were plated and 10 µg/ml pFN was added to medium along with 5% fibronectin-depleted serum, except where indicated. For some experiments with HT1080 cells, 10 µg/ml aprotinin was added during assembly.
At each time point, medium was removed and cells were washed with PBS three times. Matrix was solubilized using 200 µl DOC lysis buffer (Sechler et al., 1996). After centrifugation, the DOC-insoluble pellet was solubilized in 25 µl of 2% SDS, 20 mM Tris-HCl, pH 8.8, 2 mM PMSF, 2 mM iodoacetic acid, 2 mM N-ethylmaleimide and 2 mM EDTA. Equal volumes of DOC-insoluble samples were analyzed by SDS-PAGE using 5% polyacrylamide gels. Samples were immunoblotted with HFN7.1 concentrated hybridoma culture supernatant diluted 1:1000 (Brenner et al., 2000
) or IC3 ascites diluted 1:10,000 (Sechler et al., 1996
). DOC-soluble samples were immunoblotted in parallel with antibodies against GAPDH to ensure equal sample loading. Immunoblots were developed with SuperSignal West Pico Chemiluminescent substrate (Pierce Chemical), or for quantification, ECL plus Western Blotting Detection System (Amersham Biosciences) was used. With the ECL plus system, band intensities were measured with a Molecular Dynamics STORM system and ImageQuant software.
For all experiments, 3D matrix was incubated with 10 µg/ml rat pFN in the absence of cells to serve as a background control. For antibody blocking experiments, cells were seeded and allowed to attach for 2 hours. Rat pFN and function-blocking monoclonal antibodies against 5 (1 µg/ml SAM-1 or 4.8 µg/ml BIIG2) or
vß3 (10 µg/ml LM609) were added to the culture medium and incubated for 14-16 hours. DOC lysates were prepared and analyzed as described above. 70 kDa fragment blocking was performed by plating 3x105 CHO(B2)
5 cells on 3D substrates with 10 µg/ml rat pFN and 125 µg/ml 70 kDa fragment or the same volume of buffer. After incubation for 7 hours, DOC-soluble and DOC-insoluble samples were prepared and analyzed.
To monitor assembly by metabolic labeling, 2x105 cells [WI-38 (VA13) or NIH3T3] were seeded on 3D matrix prepared from NIH3T3 cells or fibronectin-coated surfaces in methionine-minus DMEM containing 40 µCi/ml [35S]methionine (ICN) and 5% fibronectin-depleted FBS. After incubation for 6 hours, media were collected and DOC-soluble and DOC-insoluble fractions were prepared. Fibronectin was isolated from the medium by gelatin-Sepharose affinity chromatography (Schwarzbauer, 1991) and from lysates by immunoprecipitation with anti-fibronectin antibody R457 (Aguirre et al., 1994
). Immunoprecipitates from DOC-insoluble and DOC-soluble fractions and culture media were analyzed by SDS-PAGE. Dried gels were exposed to a phosphoimager screen, data were collected by scanning with a STORM system and band intensities were quantified using ImageQuant software. Total fibronectin was calculated by summing total counts in DOC-soluble material, DOC-insoluble material and culture media.
For preparation of resuspended matrix, conditioned medium (CM) or cell lysates, WI-38(VA13) cells were grown until highly confluent. CM was collected from the cells and centrifuged to remove floating cells. Cells were then lysed in lysis buffer and lysates were incubated with fibronectin-coated 2D surfaces at 4°C overnight. 3D fibronectin matrix was prepared as described above and resuspended by scraping the 3D matrix off the surface into 1 ml DMEM plus 5% fibronectin-depleted FBS using a rubber policeman. 2.5x105 CHO(B2)5 cells were plated on 3D matrix, 2D fibronectin substrate or on 2D fibronectin substrate pre-incubated with cell lysate. In some experiments, the medium was replaced after 1 hour with CM or resuspended matrix plus 10 µg/ml rat fibronectin. After incubation for 16 hours, the DOC-insoluble and soluble fractions were prepared.
3D fibronectin matrix prepared from WI-38(VA13) cells was compressed following a procedure described (Cukierman et al., 2001). Growth Factor Reduced MatrigelTM (BD Biosciences) was diluted 1:3 in DMEM on ice. 55 µl diluted Matrigel or Matrigel containing 60 µg/ml plasma fibronectin were allowed to gel in the wells of a 24-well plate at 37°C for 15 minutes. Polymerized Matrigel was blocked with 1% BSA in PBS before use. To fix the 3D fibronectin matrix, 1 ml of 4% paraformaldehyde in PBS was added to 3D fibronectin matrix and incubated at room temperature for 30 minutes. The fixed matrix was washed four times with 1 ml PBS followed by blocking with 1% BSA in PBS. 2.5x105 CHO(B2)
5 cells were plated onto these 3D substrates and DOC lysis was performed as described above.
To block the cryptic fibronectin-binding site I9-III1, 100 µg/ml 9D2 monoclonal antibody was added to CHO(B2)5 cells plated on 3D fibronectin matrix along with 10 µg/ml rat pFN. After 6 hours, the DOC-insoluble fraction was prepared as described above.
Indirect immunofluorescence
2x105 cells were seeded on 3D matrix or fibronectin-coated 12-mm coverslips with or without 4 µg/ml exogenous fibronectin and incubated for various times. Samples were fixed and stained with the appropriate species-specific antibodies (either HFN7.1 or IC3) followed by fluorescein-conjugated goat anti-mouse IgG (Sechler et al., 1996). Images were captured using a Nikon Eclipse TE2000-U microscope and IPLab software. To analyze distributions of 3D human fibronectin matrix and exogenous rat pFN matrix, 1.5x105 CHO(B2)
5 cells were plated in medium with 5% fibronectin-depleted FBS on coverslips with either 3D human fibronectin matrix or coated with human pFN. After allowing 2 hours for cell attachment, 4 µg/ml rat fibronectin was added and cells were incubated for 15 hours. Samples were fixed in 3.7% formaldehyde in PBS followed by sequential antibody incubations. First incubation was with IC3 ascites (1:1000) at 37°C for 30 minutes followed by fluorescein-conjugated goat anti-mouse IgG for 30 minutes. Samples were rinsed thoroughly and a second incubation with IC3 ascites (1:1000) at room temperature for 10 minutes was performed to saturate any remaining anti-mouse IgG. After rinsing, samples were incubated with biotin-conjugated HFN7.1 antibody (biotinylated according to instructions of Pierce Chemical) at 37°C for 30 minutes followed by staining with rhodamine-conjugated streptavidin. Cells were analyzed using Ziess 510 confocal microscope or Nikon Eclipse TE2000-U microscope.
![]() |
Results |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
|
Increased fibril assembly by cells on 3D fibronectin matrix
Matrix assembly by cells on the 3D fibronectin matrix was compared to that by cells plated on a 2D substrate prepared by coating plastic or glass surfaces with a 10 µg/ml solution of plasma fibronectin (pFN). Cells and matrix from different species were used to differentiate cell-assembled matrix from the 3D fibronectin matrix. ELISA with monoclonal antibody HFN7.1, which inhibits cell binding to human fibronectin, showed that 2D-coated surfaces had more accessible cell binding sites than 3D matrices at all fibronectin concentrations used in this study (data not shown). WI-38 (VA-13)-transformed human lung fibroblasts on a 3D mouse fibronectin matrix assembled significantly more fibronectin fibrils. Comparison of deoxycholate (DOC) detergent-insoluble matrix formed on 2D compared to 3D substrates showed time-dependent (Fig. 2A) and cell density-dependent (Fig. 2B) differences. By indirect immunofluorescence, it was clear that these cells assembled a more extensive network when grown on the 3D fibronectin matrix (Fig. 2C, left panel) in contrast to the short fibrils around peripheries of cells growing on the 2D substrate (Fig. 2C, right panel). To verify that the increased assembly is not unique to WI-38 (VA13) cells, several other fibroblast cell lines were tested. Primary cell lines WI-38 and human dermal fibroblasts also assembled more DOC-insoluble matrix on a 3D substrate (Fig. 2D). To quantify the levels of fibronectin expression and matrix deposition on the two substrates, assembly by cells labeled with [35S]methionine was monitored. WI-38 (VA13) and NIH3T3 cells on 3D substrates assembled more than threefold the amount of DOC-insoluble [35S]fibronectin than on 2D substrates (Fig. 3A) with percentage incorporation increasing from 0.7% on 2D substrate to 3.3% on 3D matrix for WI-38 (VA-13) cells and from 0.7% to 2.2% for NIH3T3 cells. Total production of fibronectin by these cell lines did not differ significantly on 3D compared to 2D substrates (Fig. 3B). Together these results show that fibroblasts within a 3D fibronectin matrix environment are stimulated to assemble fibronectin matrix.
|
|
Partial overlap of newly assembled fibrils and 3D matrix
To control the amount and availability of fibronectin during matrix assembly, we analyzed formation of exogenous rat pFN matrix by CHO(B2)5 cells that produce very little endogenous fibronectin. Incorporation of exogenous rat pFN by CHO(B2)
5 cells on a 3D human fibronectin matrix was significantly increased compared to these cells on a 2D human pFN substrate. DOC-insoluble matrix was detectable at all time points and accumulated to high levels in a time-dependent fashion (Fig. 4A). Minimal background binding of exogenous fibronectin to the 3D matrix was observed over this time course. It has been previously shown that interactions involving the N-terminal assembly domain of fibronectin are essential for fibril formation by cells in monolayer culture (McDonald et al., 1987
; McKeown-Longo and Mosher, 1985
; Schwarzbauer, 1991
). Inclusion of excess 70 kDa fibronectin fragment, which contains the assembly domain, inhibited assembly of DOC-insoluble matrix on 3D fibronectin matrix (Fig. 4B). Therefore, assembly on 2D and 3D substrates follows similar mechanisms. Fibronectin fibril formation was distinctly different on 2D and 3D substrates. CHO(B2)
5 cells elaborated a network of extended fibrils that stretched across and between adjacent cells (Fig. 4C, left panel). In contrast, fibrils formed by cells on the 2D substrate were shorter, fewer in number, and mainly extended between cell and substrate (Fig. 4C, right panel). In these experiments, exogenous fibronectin was provided in the medium at 4 µg/ml, a concentration significantly lower than the concentrations routinely used in matrix assembly experiments, typically 50 µg/ml or more (Sechler et al., 1996
; Wu et al., 1995
). With higher concentrations of fibronectin, cells on 2D substrates usually assemble a more extensive network over this time period. Thus, in addition to increasing the rate, 3D matrix also lowers the threshold concentration needed for assembly of mature matrix.
|
Double staining of newly assembled fibrils (rat pFN) and 3D matrix fibrils (human fibronectin) showed only partial co-alignment and there were areas where rat fibronectin matrix was much denser than the 3D fibronectin matrix substrate (Fig. 5A). To determine the extent of colocalization, fluorescence intensity profiles were calculated for four areas in the merged image. Fibrils containing varying amounts of both human 3D matrix and rat fibronectin fibrils are shown under lines 1 and 2 (Fig. 5B). Some fibrils are primarily composed of newly assembled rat fibronectin (green lines). However, other regions show coincidence of rat fibronectin and human 3D matrix (red lines) suggesting that the rat fibronectin fibrils extend from the 3D matrix. Relatively homogeneous fibrils were also observed; line 3 indicates fibrils composed primarily of rat fibronectin and devoid of 3D matrix whereas line 4 crosses mostly human 3D matrix. These data indicate that the newly assembled fibrils are not simply deposited onto the 3D matrix.
|
|
Fibronectin matrix assembly is affected by cell contractility (Zhang et al., 1994; Zhong et al., 1998
). A matrix more pliable than a 2D fibronectin-coated surface may change contractility and stimulate assembly. To examine this possibility, matrix assembly was analyzed in cells growing on growth factor-reduced Matrigel matrices with or without added fibronectin. There was some stimulation of fibronectin assembly in cells on Matrigel matrices compared to cells on 2D fibronectin substrates but much less than cells on 3D fibronectin matrix (Fig. 7A). Furthermore, inclusion of fibronectin in the Matrigel matrix had no additional effect over Matrigel alone. To reduce the pliability of the 3D fibronectin matrix, it was crosslinked with 4% paraformaldehyde. Solubilized fixed 3D fibronectin matrix migrated as high molecular weight protein complexes in a 5% polyacrylamide SDS gel indicating covalent crosslinking of the matrix (data not shown). In addition, time-lapse video microscopy of cells migrating on these matrices showed significant flexibility of the 3D matrix fibrils that was reduced after fixation (data not shown). Even so, fibronectin matrix assembly on fixed 3D matrix was stimulated to a comparable degree as on native unfixed matrix (Fig. 7B).
|
Stimulatory effects of 3D matrix on integrin-dependent assembly
Integrin 5ß1 is primarily responsible for fibroblast matrix assembly. Although this integrin does not require exogenous stimulation to mediate assembly, the amount of
5ß1-dependent fibronectin matrix can be increased by treatment of cells with activating agents such as Mn2+ (Sechler et al., 1997
) or by overexpressing
5ß1 integrin (Giancotti and Ruoslahti, 1990
). Cell surface levels of
5ß1 on 2D and 3D substrates were not significantly different (data not shown). To test the idea that integrin activity is modulated in the presence of 3D matrix, we used the human fibrosarcoma cell line HT1080, which is dependent on stimulation with transcriptional activators or exogenous integrin-activators such as Mn2+ in order to assemble fibronectin matrix (Brenner et al., 2000
; McKeown-Longo and Etzler, 1987
). There was significant accumulation of exogenous rat fibronectin matrix assembled by these cells on 3D matrix in the absence of stimulants (Fig. 8A). DOC-insoluble material was detected on 2D substrate only upon treatment with 1 mM Mn2+. These results show that the presence of a 3D fibronectin matrix stimulates integrin-mediated assembly by these tumor cells.
|
Integrins other than 5ß1 are dependent on activation in order to carry out matrix assembly. We used cells expressing
vß3 integrin, which can be stimulated to form fibronectin fibrils in monolayer culture (Wu et al., 1996
). CHO(B2)
vß3 cells on 3D fibronectin matrix assembled detectable DOC-insoluble material when provided with concentrations of fibronectin as low as 5 µg/ml (Fig. 8B). In contrast, on a 2D substrate these cells did not form detectable matrix unless provided with a higher dose of fibronectin and longer incubation time combined with Mn2+ stimulation of the integrin (Fig. 8C). Assembly was dependent on integrins, as parental CHO(B2) cells which lack these receptors did not accumulate any detectable fibronectin (Fig. 8B). Function-blocking antibodies SAM-1 against
5ß1 or LM609 against
vß3 caused a dramatic reduction in matrix deposition (Fig. 8D). Similar inhibition of CHO(B2)
5-mediated assembly was observed with the BIIG2 anti-
5ß1 antibody but not with LM609 antibody (not shown), demonstrating integrin specificity for the assembly process. Together with the results from HT1080 cells, these findings suggest that a 3D matrix might stimulate fibril formation through effects on integrin activity. Furthermore, in at least two different situations, with tumor cells and with an activation-dependent integrin, a 3D microenvironment has dramatic stimulatory effects on integrin-mediated fibronectin assembly.
![]() |
Discussion |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Substantial evidence shows that fibronectin matrix assembly is dependent on productive integrin-fibronectin interactions (Bae et al., 2004; Mosher, 1993
; Schwarzbauer and Sechler, 1999
). This process is usually mediated by
5ß1 integrin binding to RGD and synergy sites in the central cell binding domain of fibronectin. Several other integrins can assemble fibronectin but only when they are appropriately stimulated to bind to fibronectin (Sechler et al., 2000
; Wu et al., 1996
; Wu et al., 1995
) or by introducing activating mutations in the integrin cytoplasmic domains (Hughes et al., 1996
). Clearly there are specific molecular requirements for productive interactions between integrins and fibronectin in order for assembly to proceed.
Here we have shown that integrin stimulation with exogenous activators is not required for fibril assembly by cells on a 3D matrix. This was true for tumor cells expressing activation-dependent 5ß1 as well as CHO transfectants expressing
vß3. Furthermore, the amount of fibronectin needed for integrin-mediated assembly was significantly lower on a 3D matrix than for cells grown on a protein-coated surface. Our results indicate that the 3D organization of the matrix fibrils plays an essential role in stimulating cell-mediated fibronectin assembly. Significant enhancement of assembly was lost when the 3D structure was disrupted or compressed or when fibronectin was presented in a non-fibrillar 3D form. We also did not detect differences in ERK/MAP kinase activation in CHO(B2)
5, HT1080 or WI-38(VA13) cells on 2D substrates or 3D matrices (unpublished observations) suggesting that these substrates did not induce detectably different levels of growth factor signaling through this pathway. Perhaps our native 3D network acts similarly to an in vitro-generated polymeric form of fibronectin, superfibronectin, that has improved adhesive activity owing to the combined effects of integrins and other non-integrin cell adhesion receptors (Morla et al., 1994
).
We expect that the effect of 3D matrix on fibronectin assembly will extend to the tissue microenvironment. In fact, our findings may help to explain the compensatory activity of vß1 integrin in
5-null mice, which allowed the embryos to survive to day E10, 1.5 days longer than embryos lacking fibronectin, the ligand for
5ß1 (Yang and Hynes, 1996
; Yang et al., 1993
). When expressed in CHO(B2) cells grown in monolayer culture,
vß1 was unable to support fibronectin matrix assembly (Zhang et al., 1993
). Embryonic tissue might induce
v integrin functions in a manner similar to the activation we observed in cells on 3D fibronectin matrix.
Dimeric fibronectin in solution has a compact structure that becomes extended during fibril formation (Baneyx et al., 2002; Johnson et al., 1999
; Schwarzbauer and Sechler, 1999
). In a 3D matrix, dimeric fibronectin localized to the cell surface by integrin binding would also be in contact with matrix fibronectin and this interaction could lead to an activating conformational change in the fibronectin. Newly exposed fibronectin binding sites would then promote fibronectin-fibronectin interactions and increase the rate of fibril formation. In support of this mechanism, treatments and mutations that alter fibronectin conformation have been shown to increase fibronectin incorporation into matrix in monolayer culture (McKeown-Longo and Mosher, 1985
; Morla et al., 1994
; Sechler et al., 1996
). Furthermore, local effects of fibronectin conformation have recently been proposed to explain the increased efficiency of exogenous fibronectin assembly by cells growing on vitronectin (Bae et al., 2004
). However, fixed 3D fibronectin matrix maintained the ability to stimulate assembly and 9D2 antibody binding to the 3D fibronectin matrix did not detectably block assembly. These results suggest that major rearrangements of fibronectin organization within the 3D matrix do not account for increased fibronectin matrix assembly.
Mechanistically, the effects of a 3D network on fibronectin assembly are probably due to presentation of organized ligand binding sites. Integrins in solution undergo conformational changes when treated with ligand mimetics, activating agents or physiological ligands (Adair et al., 2005; Mould et al., 2003
; Takagi and Springer, 2002
; Takagi et al., 2003
; Xiong et al., 2001
; Xiong et al., 2002
). Such conformational changes in integrins may affect both the specificity and affinity of ligand recognition (Frelinger et al., 1991
), perhaps through formation of regulatory cytoplasmic complexes. In fact, talin binding to the ß integrin cytoplasmic domain has recently been shown to stimulate inactive integrins (Tadokoro et al., 2003
) and talin is found in 3D ECM adhesion complexes (Cukierman et al., 2001
). Initial formation of activating cytoplasmic complexes may be sufficient to maintain increased integrin activity in cells surrounded by existing fibronectin matrix. Mn2+ treatment stimulated matrix assembly by HT1080 cells on 2D substrates but only had a minimal effect on cells in 3D fibronectin matrix. This observation suggests that, within a 3D substrate, integrins may already be in an active form.
Stimulatory effects of a 3D matrix provide additional opportunities for fibronectin assembly in vivo and this could have a profound impact on cell behavior. For example, some tumor cells have lost their ability to efficiently assemble their own matrix in culture owing to reduced expression of integrins and fibronectin or reduced integrin activity after oncogenic transformation (Brenner et al., 2000; Hynes, 1990
; Plantefaber and Hynes, 1989
; Ruoslahti, 1999
). Our results suggest that when such cells are placed in the appropriate physiological environment, they might be stimulated to incorporate fibronectin into matrix. This effect could contribute to the growth and spread of tumors in vivo. Understanding the regulatory effects of 3D matrix on fibronectin assembly may shed light on the modulation of cell function in specific in vivo situations.
![]() |
Acknowledgments |
---|
![]() |
References |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Adair, B. D., Xiong, J. P., Maddock, C., Goodman, S. L., Arnaout, M. A. and Yeager, M. (2005). Three-dimensional EM structure of the ectodomain of integrin {alpha}V{beta}3 in a complex with fibronectin. J. Cell Biol. 168, 1109-1118.
Aguirre, K. M., McCormick, R. J. and Schwarzbauer, J. E. (1994). Fibronectin self-association is mediated by complementary sites within the amino-terminal one-third of the molecule. J. Biol. Chem. 269, 27863-27868.
Bae, E., Sakai, T. and Mosher, D. F. (2004). Assembly of exogenous fibronectin by fibronectin-null cells is dependent on the adhesive substrate. J. Biol. Chem. 279, 35749-35759.
Baneyx, G., Baugh, L. and Vogel, V. (2002). Fibronectin extension and unfolding within cell matrix fibrils controlled by cytoskeletal tension. Proc. Natl. Acad. Sci. USA 99, 5139-5143.
Brenner, K. A., Corbett, S. A. and Schwarzbauer, J. E. (2000). Regulation of fibronectin matrix assembly by activated Ras in transformed cells. Oncogene 19, 3156-3163.[CrossRef][Medline]
Chen, L. B., Murray, A., Segal, R. A., Bushnell, A. and Walsh, M. L. (1978). Studies on intercellular LETS glycoprotein matrices. Cell 14, 377-391.[CrossRef][Medline]
Chernousov, M. A., Fogerty, F. J., Koteliansky, V. E. and Mosher, D. F. (1991). Role of the I-9 and III-1 modules of fibronectin in formation of an extracellular fibronectin matrix. J. Biol. Chem. 266, 10851-10858.
Cukierman, E., Pankov, R., Stevens, D. R. and Yamada, K. M. (2001). Taking cell-matrix adhesions to the third dimension. Science 294, 1708-1712.
Fogerty, F. J., Akiyama, S. K., Yamada, K. M. and Mosher, D. F. (1990). Inhibition of binding of fibronectin to matrix assembly sites by anti-integrin (alpha 5 beta 1) antibodies. J. Cell Biol. 111, 699-708.[Abstract]
Frelinger, A. L., 3rd, Du, X. P., Plow, E. F. and Ginsberg, M. H. (1991). Monoclonal antibodies to ligand-occupied conformers of integrin alpha IIb beta 3 (glycoprotein IIb-IIIa) alter receptor affinity, specificity, and function. J. Biol. Chem. 266, 17106-17111.
Giancotti, F. G. and Ruoslahti, E. (1990). Elevated levels of the alpha 5 beta 1 fibronectin receptor suppress the transformed phenotype of Chinese hamster ovary cells. Cell 60, 849-859.[CrossRef][Medline]
Hughes, P. E., Diaz-Gonzalez, F., Leong, L., Wu, C., McDonald, J. A., Shattil, S. J. and Ginsberg, M. H. (1996). Breaking the integrin hinge. A defined structural constraint regulates integrin signaling. J. Biol. Chem. 271, 6571-6574.
Hynes, R. O. (1990). Fibronectins. New York: Springer-Verlag.
Ilic, D., Kovacic, B., Johkura, K., Schlaepfer, D. D., Tomasevic, N., Han, Q., Kim, J. B., Howerton, K., Baumbusch, C., Ogiwara, N. et al. (2004). FAK promotes organization of fibronectin matrix and fibrillar adhesions. J. Cell Sci. 117, 177-187.
Johnson, K. J., Sage, H., Briscoe, G. and Erickson, H. P. (1999). The compact conformation of fibronectin is determined by intramolecular ionic interactions. J. Biol. Chem. 274, 15473-15479.
Ly, D. P., Zazzali, K. M. and Corbett, S. A. (2003). De novo expression of the integrin alpha5beta1 regulates alphavbeta3-mediated adhesion and migration on fibrinogen. J. Biol. Chem. 278, 21878-21885.
McDonald, J. A., Quade, B. J., Broekelmann, T. J., LaChance, R., Forsman, K., Hasegawa, E. and Akiyama, S. (1987). Fibronectin's cell-adhesive domain and an amino-terminal matrix assembly domain participate in its assembly into fibroblast pericellular matrix. J. Biol. Chem. 262, 2957-2967.
McKeown-Longo, P. J. and Mosher, D. F. (1983). Binding of plasma fibronectin to cell layers of human skin fibroblasts. J. Cell Biol. 97, 466-472.[Abstract]
McKeown-Longo, P. J. and Mosher, D. F. (1985). Interaction of the 70,000-mol-wt amino-terminal fragment of fibronectin with the matrix-assembly receptor of fibroblasts. J. Cell Biol. 100, 364-374.[Abstract]
McKeown-Longo, P. J. and Etzler, C. A. (1987). Induction of fibronectin matrix assembly in human fibrosarcoma cells by dexamethasone. J. Cell Biol. 104, 601-610.[Abstract]
Morla, A., Zhang, Z. and Ruoslahti, E. (1994). Superfibronectin is a functionally distinct form of fibronectin. Nature 367, 193-196.[CrossRef][Medline]
Mosher, D. F. (1993). Assembly of fibronectin into extracellular matrix. Curr. Opin. Struct. Biol. 3, 214-222.[CrossRef]
Mould, A. P., Symonds, E. J., Buckley, P. A., Grossmann, J. G., McEwan, P. A., Barton, S. J., Askari, J. A., Craig, S. E., Bella, J. and Humphries, M. J. (2003). Structure of an integrin-ligand complex deduced from solution x-ray scattering and site-directed mutagenesis. J. Biol. Chem. 278, 39993-39999.
Plantefaber, L. C. and Hynes, R. O. (1989). Changes in integrin receptors on oncogenically transformed cells. Cell 56, 281-290.[CrossRef][Medline]
Ruoslahti, E. (1999). Fibronectin and its integrin receptors in cancer. Adv. Cancer Res. 76, 1-20.[Medline]
Schreiner, C. L., Bauer, J. S., Danilov, Y. N., Hussein, S., Sczekan, M. M. and Juliano, R. L. (1989). Isolation and characterization of Chinese hamster ovary cell variants deficient in the expression of fibronectin receptor. J. Cell Biol. 109, 3157-3167.[Abstract]
Schwarzbauer, J. E. (1991). Identification of the fibronectin sequences required for assembly of a fibrillar matrix. J. Cell Biol. 113, 1463-1473.[Abstract]
Schwarzbauer, J. E. and Sechler, J. L. (1999). Fibronectin fibrillogenesis: a paradigm for extracellular matrix assembly. Curr. Opin. Cell. Biol. 11, 622-627.[CrossRef][Medline]
Sechler, J. L., Takada, Y. and Schwarzbauer, J. E. (1996). Altered rate of fibronectin matrix assembly by deletion of the first type III repeats. J. Cell Biol. 134, 573-583.[Abstract]
Sechler, J. L., Corbett, S. A. and Schwarzbauer, J. E. (1997). Modulatory roles for integrin activation and the synergy site of fibronectin during matrix assembly. Mol. Biol. Cell 8, 2563-2573.
Sechler, J. L., Corbett, S. A., Wenk, M. B. and Schwarzbauer, J. E. (1998). Modulation of cell-extracellular matrix interactions. Ann. New York Acad. Sci. 857, 143-154.
Sechler, J. L., Cumiskey, A. M., Gazzola, D. M. and Schwarzbauer, J. E. (2000). A novel RGD-independent fibronectin assembly pathway initiated by alpha4beta1 integrin binding to the alternatively spliced V region. J. Cell Sci. 113, 1491-1498.
Tadokoro, S., Shattil, S. J., Eto, K., Tai, V., Liddington, R. C., de Pereda, J. M., Ginsberg, M. H. and Calderwood, D. A. (2003). Talin binding to integrin beta tails: a final common step in integrin activation. Science 302, 103-106.
Takagi, J. and Springer, T. A. (2002). Integrin activation and structural rearrangement. Immunol. Rev. 186, 141-163.[CrossRef][Medline]
Takagi, J., Strokovich, K., Springer, T. A. and Walz, T. (2003). Structure of integrin alpha5beta1 in complex with fibronectin. EMBO J. 22, 4607-4615.
Tamariz, E. and Grinnell, F. (2002). Modulation of fibroblast morphology and adhesion during collagen matrix remodeling. Mol. Biol. Cell 13, 3915-3929.
Vlodavsky, I., Lui, G. M. and Gospodarowicz, D. (1980). Morphological appearance, growth behavior and migratory activity of human tumor cells maintained on extracellular matrix versus plastic. Cell 19, 607-616.[CrossRef][Medline]
Wenk, M. B., Midwood, K. S. and Schwarzbauer, J. E. (2000). Tenascin-C suppresses Rho activation. J. Cell Biol. 150, 913-920.
Wennerberg, K., Lohikangas, L., Gullberg, D., Pfaff, M., Johansson, S. and Fassler, R. (1996). Beta 1 integrin-dependent and -independent polymerization of fibronectin. J. Cell Biol. 132, 227-238.[Abstract]
Wierzbicka-Patynowski, I. and Schwarzbauer, J. E. (2002). Regulatory role for SRC and phosphatidylinositol 3-kinase in initiation of fibronectin matrix assembly. J. Biol. Chem. 277, 19703-19708.
Wierzbicka-Patynowski, I. and Schwarzbauer, J. E. (2003). The ins and outs of fibronectin matrix assembly. J. Cell. Sci. 116, 3269-3276.
Wu, C., Keivens, V. M., O'Toole, T. E., McDonald, J. A. and Ginsberg, M. H. (1995). Integrin activation and cytoskeletal interaction are essential for the assembly of a fibronectin matrix. Cell 83, 715-724.[CrossRef][Medline]
Wu, C., Hughes, P. E., Ginsberg, M. H. and McDonald, J. A. (1996). Identification of a new biological function for the integrin alpha v beta 3, initiation of fibronectin matrix assembly. Cell Adhes. Commun. 4, 149-158.[Medline]
Xiong, J. P., Stehle, T., Diefenbach, B., Zhang, R., Dunker, R., Scott, D. L., Joachimiak, A., Goodman, S. L. and Arnaout, M. A. (2001). Crystal structure of the extracellular segment of integrin alphaVbeta3. Science 294, 339-345.
Xiong, J. P., Stehle, T., Zhang, R., Joachimiak, A., Frech, M., Goodman, S. L. and Arnaout, M. A. (2002). Crystal structure of the extracellular segment of integrin alpha Vbeta3 in complex with an Arg-Gly-Asp ligand. Science 296, 151-155.
Yang, J. T. and Hynes, R. O. (1996). Fibronectin receptor functions in embryonic cells deficient in alpha 5 beta 1 integrin can be replaced by alpha V integrins. Mol. Biol. Cell 7, 1737-1748.[Abstract]
Yang, J. T., Rayburn, H. and Hynes, R. O. (1993). Embryonic mesodermal defects in alpha 5 integrin-deficient mice. Development 119, 1093-1105.
Zhang, Q., Checovich, W. J., Peters, D. M., Albrecht, R. M. and Mosher, D. F. (1994). Modulation of cell surface fibronectin assembly sites by lysophosphatidic acid. J. Cell Biol. 127, 1447-1459.[Abstract]
Zhang, Z., Morla, A. O., Vuori, K., Bauer, J. S., Juliano, R. L. and Ruoslahti, E. (1993). The alpha v beta 1 integrin functions as a fibronectin receptor but does not support fibronectin matrix assembly and cell migration on fibronectin. J. Cell Biol. 122, 235-242.[Abstract]
Zhong, C., Chrzanowska-Wodnicka, M., Brown, J., Shaub, A., Belkin, A. M. and Burridge, K. (1998). Rho-mediated contractility exposes a cryptic site in fibronectin and induces fibronectin matrix assembly. J. Cell Biol. 141, 539-551.
|