1 Department of Pathology, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan
2 CREST Research Project, Japan Science and Technology Corporation, 4-1-6 Kawaguchi, Saitama, 332-0012, Japan
3 Shinanomachi Research Park, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan
4 Department of Biosystems Science, School of Advanced Sciences, The Graduate University for Advanced Studies (Sokendai), Shonan Village, Hayama, Kanagawa 240-0193, Japan
5 Bioinformatics Business Promotion Department, NEC Corporation, 7-1 Shiba 5-chome, Minato-ku, Tokyo 108-8001, Japan
6 Fundamental and Environmental Research Laboratories, NEC Corporation, 34 Miyukigaoka, Tsukuba, Ibaraki 305-8501, Japan
7 Cell bank laboratory, Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan
* Authors for correspondence (e-mail: kuroda{at}tokyo-med.ac.jp; tanabe_hideyuki{at}soken.ac.jp)
Accepted 23 August 2004
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Summary |
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Key words: Chromosome territory, Adipocyte differentiation, TLS-CHOP
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Introduction |
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Specific chromosomal translocations have consistently been found in particular cancers and might promote tumorigenesis through the activation of specific oncogenes or the creation of fusion proteins (Rabbitts, 1994). Human myxoid liposarcomas are associated with the chromosomal translocation t(12;16)(q13.3;p11.2), which creates a chimeric oncogene comprising part of the TLS/FUS gene found at 16p11.2 and part of the CHOP gene found at 12q13.3 (Aman et al., 1992
; Crozat et al., 1993
; Eneroth et al., 1990
; Rabbitts et al., 1993
). The resultant fusion protein is crucial to the transforming activity of the translocation, through its promotion of the unscheduled expression of the adipocyte differentiation gene DOL54 (Kuroda et al., 1997
; Kuroda et al., 1999
). The reasons underlying the occurrence of this specific translocation in liposarcomas, however, have remained elusive.
Recent studies have reported that the close juxtaposition of interphase chromosomes plays an important role in such basic cellular processes as gene expression. Moreover, the induction of chromosomal translocations is influenced by proximity of chromosomes, with chromosomes in close proximity to one other presumably more likely to undergo translocations than those that are further apart (Bickmore and Teague, 2002; Parada and Misteli, 2002
; Sachs et al., 1997
). Interestingly, such proximity effects have been described in analyses of cancer-causing translocations involved in both leukemia and Ewing sarcoma (Kozubek et al., 1999
; Taslerova et al., 2003
).
In this study, we examined the relative and radial positioning of human chromosomes 12 and 16 in both preadipocytes and adipocytes to address the question of whether or not chromosome-territory (CT) repositioning occurs during adipocyte differentiation. We observed an alteration in the positioning of these CTs, suggesting that the translocation t(12;16), which might play a key role in liposarcoma tumorigenesis, is induced by the alteration in CT location. In addition, these data indicate that chromatin and nuclear compartments are dynamic during cell differentiation, and that these changes might play a role in the regulation of transcriptional activity in chromatin.
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Materials and Methods |
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Probe preparation, three-dimensional fluorescence in-situ hybridization and fluorescence detection
In order to obtain three-dimensionally preserved cell nuclei, cells were cultured on coverslip slides and fixed in 4% paraformaldehyde in 0.3x PBS. Permeabilization was performed as previously described (Solovei et al., 2002), by treating the cells with 0.5% Triton X-100 in PBS and then 20% glycerol in PBS, followed by repeated freeze-thaw cycles in liquid nitrogen before a final incubation in 0.1 N HCl.
For the delineation of the human chromosome-12 territory (CT12) and chromosome-16 territory (CT16), we used whole-chromosome painting probes provided by T. Cremer (Ludwig-Maximilians University of Munich, Munich, Germany). Probe labeling was performed by DOP- (degenerated oligonucleotide primer) PCR (Telenius et al., 1992) in the presence of biotin-16-dUTP for human chromosome 12 or digoxigenin-11-dUTP (DIG-11-dUTP) for human chromosome 16 (both from Roche). Three-dimensional fluorescence in-situ hybridization (3D-FISH) and the detection of labeled probes were performed according to protocols described elsewhere (Cremer et al., 2001
; Solovei et al., 2002
). Briefly, biotinylated human chromosome 12 was detected by avidin-conjugated fluorescein isothiocyanate (FITC) and biotinylated goat-anti-avidin antibody (both from Vector), followed by another round of avidin-FITC binding. Simultaneously, DIG-labeled human chromosome 16 was detected in a similar fashion using a rabbit-anti-DIG antibody (Sigma) and Cy3-labeled goat-anti-rabbit antibody (Amersham Pharmacia Biotech). DNA counterstaining was carried out using TOPRO-3 (Cy5-like fluorescence peak; Molecular Probes), and slides were mounted in Vectashield medium (Vector).
Confocal image
Serial nuclear images were acquired with an axial separation of 200 nm using a confocal laser-scanning microscope (LSM 410; Carl Zeiss) equipped with a 63x/1.4 Plan-Apochromat objective. For each optical section, sequential images were recorded for all three fluorochromes (FITC, Cy3 and Cy5). Stacks of 8-bit grayscale two-dimensional (2D) images were obtained with a pixel size of 66 nm and with 512x512 pixels in each channel. The image stacks were processed with Adobe Photoshop 7 and the distances between fluorescence peak centers (FPCs) were measured after converting image stacks into 256x256 pixels. Three-dimensional (3D) reconstructions of hybridized nuclear image stacks were created using Amira 3.0 TGS (http://www.amiravis.com/) software. Amira software was used only for visualization, not for data analysis.
Segmentation
A probability density of intensity was modeled by a finite normal mixture with m components (m=3 or 4):
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Each pixel in 3D space was classified based on a posterior probability (i,j) calculated as:
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Distance measurement
FPCs of the CTs were detected in 3D space. To avoid the effects of local fluctuation, an Epanechnikov filter (bandwidth 0.65 µm) was applied. We defined the pair of chromosomes 12 and 16 with the minimum FPC distance as the proximal pair and the other pair as the distal pair. The distance between CT12 and CT16 of the proximal pair was normalized using the standardized radius of the nucleus. We simulated the distribution of the normalized proximal-pair distances using Monte Carlo simulation (Kozubek et al., 2002) and compared the simulation results with experimental results. The distribution of normalized proximal-pair distances was simulated as follows. First, we generated the radial positions of four FPCs (two for CT12 and two for CT16) in a unit 2D disc using a random-number generator modulated by the experimental radial distributions. Second, the positions were determined assuming a uniform angular distribution (Kozubek et al., 2002
). Third, the CT12-CT16 pair with the minimum distance of the two possible combinations was selected and the proximal distance was determined. After 100,000 repetitions, the distributions of the proximal-pair distances were determined.
Normalization of the nucleus size and shape
To remove the effects of size and shape change during cell differentiation, we standardized the nucleus size and shape as follows.
Statistical analysis
We used 38 preadipocyte cells and 41 adipocyte cells for the distance and radial distribution analysis. Welch's t test was used to determine the significance of differences in the proximal-pair distance, and the Kolmogorov-Smirnov (KS) test was applied to the radial distribution differences. The statistical analysis software R version 1.8.1 (http://www.r-project.org/) was used.
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Results |
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Relative 3D positioning of the human CT12 and CT16 in preadipocytes and adipocytes
We performed 3D-FISH in 3D-preserved fixed nuclei using painting probes for human chromosomes 12 and 16. Both preadipocyte and adipocyte cells were arrested in the G0/G1 phase in response to cell confluence. Fluorescent signals from both human CT12 and human CT16 were successfully visualized using a two-color analysis, with green and red representing chromosomes 12 and 16, respectively.
We observed the nuclei under a microscope to determine whether or not there was a close association between CT12 and CT16. By visual inspection, we could clearly detect a close association between one CT12 and one CT16 in 81% (33/41) of adipocyte nuclei, whereas this association was observed only in 45% (17/38) of preadipocyte nuclei. Next, we imaged nuclei with a confocal laser-scanning microscope for a more precise quantitative evaluation of the distances between the CTs. A series of optical sections was recorded for each cell, with successive sections separated by an axial distance of 200 nm. All three fluorochromes were recorded sequentially for each section using a box size of 512x512 pixels. A representative z-axis series and 3D reconstruction of both preadipocyte and adipocyte nuclei are displayed in Fig. 2.
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For a quantitative evaluation of relative CT positioning, we first measured CT and nucleus size in both preadipocytes and adipocytes (Table 1). We found that the nucleus size was reduced by approximately 10% during adipocyte differentiation. In addition, the CT16 size increased by 50%, whereas the CT12 size showed no significant changes. Furthermore, the nuclei of preadipocyte and adipocyte cells exhibited a range of shapes. Therefore, to eliminate the effects of size and shape change of CTs during cell differentiation, we standardized the nucleus size (Fig. 3). We used the minimum distance between the FPCs of all four possible pairs of CT12 and CT16 in each nucleus as a measure of the level of CT association (Fig. 4A,B). The mean value for the minimum FPC distance relative to the standardized 2D nucleus radius in adipocyte nuclei was significantly smaller than that in preadipocyte nuclei (P=0.016) (Fig. 4C). These results suggest that repositioning of one pair of CTs occurs during adipocyte differentiation. This proximity effect (Fig. 4), as well as the magnification of CT16 (Table 1), substantially increases the probability of an interaction between CT12 and CT16, and is likely to lead to a t(12;16) translocation during adipocyte differentiation. Although the parental origin of this pair of chromosomes is unknown, this raises the intriguing possibility that an association between a specific combination of chromosomes of paternal or maternal origin is established by as yet unknown epigenetic mechanisms.
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Radial distribution of the CT12 and CT16 does not affect their relative positioning during adipocyte differentiation
Previous studies have showed that the radial rearrangement of genetic structures changes during myogenesis (Chaly and Munro, 1996) and granulopoesis (Bartova et al., 2002
; Bartova et al., 2001
; Bartova et al., 2000b
). Therefore, we next examined the radial distributions of CT12 and CT16 in preadipocyte and adipocyte cells. We investigated total radial distributions over the standardized 2D nucleus and compared them for the cell types studied using the KS test. Although there were no significant differences between CT12 and CT16 during adipocyte differentiation, the means of the total radial distribution appeared to shift towards the center for CT16 (from <r>=59.0±22.0 to 56.8±21.5, P>0.49, where <r> stands for the mean radial position) and the mean of the total radial distribution for CT12 shifted toward the periphery (from <r>=57.7±21.4 to 0.59.8±21.4, P>0.7) (Fig. 5). This shift of the CT might account for the reduction in relative distances between CT12 and CT16.
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Thus, to determine whether the difference of minimum FPC distances described above was affected by the fluctuation of the radial distribution of CT12 and CT16 during cell differentiation, we conducted a Monte Carlo simulation (Kozubek et al., 2002). The minimum distances obtained through the simulation were not significantly different (0.45±0.25 for adipocyte and 0.46±0.26 for preadipocyte). Thus, the change in distance between CT12 and CT16 during adipocyte differentiation was not caused by changes in radial distributions.
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Discussion |
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In this study, we evaluate the arrangement of CT12 and CT16 in preadipocyte and adipocyte cells. Interestingly, we observed that the minimum FPC distance of CT12 and CT16 changed during adipogenesis. Furthermore, we examined whether or not the total radial distribution of CT12 and CT16 changed during adipogenesis. We could not detect a significant radial shift between the two cell types using the KS test (Fig. 5). Thus, we conclude that the relative positioning of CT12 and CT16 was altered in during adipogenesis. The reasons for the difference in behavior between chromosomes 12 and 16 are at present unknown. However, it is possible that the difference is due to variations in local gene expression from each chromosome.
The process of cellular differentiation represents a remarkably coordinated program of gene regulation that directs multipotent stem-cell precursors down various lineages into fully mature and functionally distinct cell types. During adipocyte differentiation, many genes have been shown to be regulated in a differentiation-dependent manner. It is has been clearly shown that adipogenic transcription factors such as C/EBP and PPAR play an important role in the regulation of gene expression through conventional genetic mechanisms. However, recent reports indicate that the spatial arrangement of chromatin in the nucleus is correlated with cell differentiation (Bartova et al., 2002; Bartova et al., 2001
; Bartova et al., 2000a
; Manuelidis, 1990
). Moreover, gene positioning and heterochromatin-mediated gene silencing appear to play an important role in cell differentiation (Bartova et al., 2002
). Our results and other studies of CTs (Mahy et al., 2002a
; Mahy et al., 2002b
) suggest that chromosome distribution might also control epigenetic mechanisms that affect regulation of genes. Thus, changes in CT location might act as an epigenetic factor that functions on a different level than the genetic code.
The relative positioning of CTs with respect to one other is known to influence the translocation frequencies between two chromosomes. For example, human myxoid and round cell liposarcomas are associated with specific chromosomal translocations (Sreekantaiah et al., 1992). The TLS-CHOP fusion gene derived from t(12;16) is present in 95-98% of myxoid and round cell liposarcomas but, in rare cases, a variant t(12;22) translocation is observed that results in the EWS-CHOP fusion gene (Hosaka et al., 2002
). The TLS-CHOP chimeric protein has transforming activity (Kuroda et al., 1997
) and induces the expression of the DOL54 gene, which is normally associated with adipocyte differentiation (Kuroda et al., 1999
). In addition, histological diagnosis of myxoid liposarcomas reveals the presence of immature adipose cells called lipoblasts, suggesting that myxoid liposarcomas come from an immature mesechymal or adipocytic cell lineage. However, it is still unknown why the chromosomal translocation t(12;16)(q13.3;p11.2) and the creation of the TLS-CHOP fusion gene occur specifically in liposarcomas. Our observations demonstrate that the minimum FPC distance between CT12 and CT16 is reduced, and the size of CT16 is magnified during adipocyte differentiation. These phenomena might lead to the t(12;16) translocation event specific to liposarcomas.
In conclusion, we have demonstrated that the particular CTs involved in a specific translocation event implicated in cancer development can physically associate with one another following differentiation in human cell lines. These results also suggest that the t(12;16) translocation, which has been implicated in liposarcoma tumorigenesis, occurs because of an alteration in chromosome location.
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Acknowledgments |
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