Department of Molecular Biosciences, University of Adelaide, Adelaide 5005, South Australia
* Contributed equally to this work
Current address: Department of Ophthalmology, Flinders University of South Australia, Bedford Park, SA 5042, Australia
Current address: Laboratory of Cellular and Developmental Biology, Section of Mammalian Development, NIDDK, NIH, Bethesda, MD 50892, USA
¶Author for correspondence (e-mail: peter.rathjen{at}adelaide.edu.au)
Accepted September 24, 2001
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SUMMARY |
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Key words: Pluripotent, ES cells, EPL cells, ICM, Primitive ectoderm, Mammalian development, Psc1, CRTR-1, PRCE, Differential gene expression
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Introduction |
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The first differentiation event of mouse embryogenesis entails the elaboration of extra-embryonic lineages by formation of trophectoderm from outer cells of the embryo. Inner cells, which retain pluripotent differentiation capability, form the inner cell mass (ICM) of the blastocyst. At 4 days post coitum (d.p.c.), ICM cells lining the blastocoelic cavity differentiate into primitive endoderm, the precursor of extra-embryonic lineages including visceral and parietal endoderm. Following this differentiation, pluripotent cells within the mouse embryo are referred to generically as the epiblast. At around the time of implantation (4.5 d.p.c.), ICM cells commence a complex developmental program resulting in the formation of a second pluripotent cell population, the primitive ectoderm. Rapid proliferation, in which cell cycles as short as 5 hours have been reported (Snow, 1977
), results in expansion of the pluripotent cell population from
25 cells at 4.5 d.p.c. to 660 cells by 6.5 d.p.c. (Snow, 1977
). Transplantation experiments (Beddington, 1983a
) reveal alteration in the developmental potential of pre- and post-implantation pluripotent cells, and this is accompanied by alterations in gene expression (Haub and Goldfarb, 1991
; Hebert et al., 1991
; Rogers et al., 1991
). Formation of the proamniotic cavity within the epiblast, apparently as a result of cell apoptosis (Coucouvanis and Martin, 1995
), is accompanied by reorganization of the pluripotent cells into a pseudostratified epithelial monolayer. The primitive ectoderm that results from these cellular events comprises several thousand pluripotent cells by 7.0 d.p.c. that are unspecified until at least 6.0-6.5 d.p.c. (Snow, 1977
; Beddington, 1981
; Beddington, 1982
; Beddington, 1983b
; Lawson et al., 1991
) and which, during gastrulation, give rise to the three germ layers of the mammalian embryo.
Pluripotent cells are receiving considerable attention owing to the recognition that manipulation of pluripotent cell lines in vitro might provide a route to therapeutic cell production (Rathjen et al., 1998; Smith, 1998
). Pluripotent cell lines with different cellular origins have been reported. Of these, mouse embryonic stem (ES) cells are stable lines that share many properties with ICM/early epiblast cells, including the ability to differentiate into all embryonic cell types including the germ line (Evans and Kaufman, 1981
; Martin, 1981
; Rathjen et al., 1999
). Pluripotent embryonal carcinoma (EC) lines have been derived from human and mouse teratocarcinomas (Pera et al., 1990
) and have diverse properties that may be explained by their different cellular origins and extended deregulated growth in vitro and in vivo. Pluripotent embryonic germ (EG) cell lines of mouse and human origin are derived by the reversion of primordial germ cells in vitro and share many properties with mouse ES cells (Resnick et al., 1992
; Matsui et al., 1992
; Shamblott et al., 1998
). Finally, human ES cells, which are of considerable therapeutic potential, appear to be substantially equivalent but in several respects distinct from mouse ES cells (Thomson et al., 1998
; Reubinoff et al., 2000
). The cellular origin and identity of pluripotent cell lines are likely to affect their growth and differentiation characteristics in vitro. Recognition of pluripotent cell lines in vitro, identification of their closest embryological equivalents and investigation of their interrelationships within and across species is therefore of considerable importance. One approach is the identification of genes that are differently expressed within distinct pluripotent cell populations in vivo.
Although pluripotent cells are fundamental to mammalian embryogenesis, relatively little is known of the developmental behaviour or molecular profile of these cells. Several lines of evidence, including cell potency studies and the phenotypes of mutant embryos, suggest that progression from ICM to primitive ectoderm entails the progressive formation of temporally distinct pluripotent cell populations (Pelton et al., 1998). However, molecular characterization of these postulated populations has not been possible because few markers delineating this time in embryogenesis have been described. Of the reported proteins, the POU domain transcription factor Oct4 can be used to recognize all pluripotent cell populations (Rosner et al., 1990
; Scholer et al., 1990
), but only the Fgf5 (Haub and Goldfarb, 1991
; Hebert et al., 1991
), Rex1 (Rogers et al., 1991
) and Gbx2 (Chapman et al., 1997
) genes are expressed differently in the ICM and primitive ectoderm. Moreover, the temporal expression of these genes in the pluripotent cell pool in vivo has only been crudely assessed.
We have previously described an in vitro culture system that results in lineage specific differentiation of mouse ES cells to a second pluripotent cell population termed early primitive ectoderm-like (EPL) cells (Rathjen et al., 1999; Lake et al., 2000
). Analysis of gene expression markers, morphology, cytokine responsiveness and differentiation potential in vivo and in vitro suggests that EPL cells are most closely related to cells of the embryonic primitive ectoderm. As a progressive event, which occupies several days in vitro, the ES-EPL cell conversion can be used to model the differentiation of ICM to primitive ectoderm in vivo and provides a system for the identification of differently expressed genes within pluripotent cell populations of the ICM and primitive ectoderm. In contrast to embryonic tissue, starting material is not limited, whereas the homogeneity and relative synchrony of the cell transition minimize difficulties associated with culture heterogeneity, including persistence of undifferentiated ES cell nests, during spontaneous ES cell differentiation in vitro (Rathjen et al., 1990
). Genes with temporally regulated expression during differentiation of the ICM to primitive ectoderm in vivo potentially provide a route to molecular identification of transient pluripotent cell populations in the embryo and their cellular equivalents in vitro. Furthermore, these genes would enable detailed mapping of the ES-EPL cell transition in vitro onto embryonic cell populations.
In this work, we used conventional differential display PCR (ddPCR) (Liang and Pardee, 1992) and modified ddPCR (internal display PCR (idPCR)) techniques to identify three novel genes that are expressed differently in ES and EPL cells: Periimplantation Stem Cell 1 (Psc1), Cp2-Related Transcriptional Repressor-1 (CRTR-1) and Protein Related to Cut1/ESP1 (PRCE). Detailed expression mapping of these genes and the reported markers Fgf5 and Rex1 in vitro and in vivo revealed that expression of each gene was restricted to a temporally distinct subpopulation of pluripotent cells within the Oct4+ pool. There was a correlation between expression of these genes in vitro and in vivo, and between alterations in gene expression and key developmental events in vivo. Characterization of these genes provides direct molecular evidence for the identification of EPL cells as like primitive ectoderm, supports the ES-EPL cell transition as a model for ICM to primitive ectoderm development in vivo and confirms the formation of temporally distinct pluripotent cell populations as the ICM differentiates to form primitive ectoderm both in the embryo and in vitro.
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Materials and Methods |
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Embryos used for in situ hybridization were obtained from time-mated Swiss females. Mice were maintained on a 12 hour light/12 hour dark cycle and supplied with food and water ad libitum. Embryo stages are represented as d.p.c., assuming that fertilization occurred at the midnight preceding the discovery of the vaginal plug. Isolation and dissection of pre-implantation and post-implantation embryos were carried out according to Hogan et al. (Hogan et al., 1994) in dissection buffer (80% ES Dulbeccos Modified Eagles Medium (DMEM) (Gibco/BRL), 20% foetal calf serum (FCS) (Commonwealth Serum Laboratories), 10 mM HEPES pH 7.4), except that 5.0-5.5 d.p.c. embryos were removed from individual implantation sites by sectioning the uterus along the mesometrial side of the implantation site, prising open the decidua and shelling out the embryo with sharpened watchmakers forceps.
Differential display PCR (ddPCR)
Cytoplasmic RNA was isolated using the method of Edwards et al. (Edwards et al., 1985) from MBL5 (Psc1) or D3 (CRTR1, PRCE) ES cells and derivative EPL cells. EPL cells were formed and cultured for 2, 4 and 6 days in medium supplemented with 50% MEDII or 50% MEDII and 1000 units ml1 LIF (Rathjen et al., 1999
). EPL cells were passaged every two days. All pluripotent cells were cultured in gelatine-treated tissue-culture-grade plastic ware in the absence of feeders.
For ddPCR (Liang and Pardee, 1992) and idPCR, 2 µg total RNA was denatured at 65°C for 10 minutes and added to a 25 µl reverse transcription reaction mix containing 50 mM Tris-HCl pH 8.5, 6 mM MgCl2, 40 mM KCl, 1 mM DTT, 1.5 mM each dNTP, 40 U RNasin and 15 U AMV reverse transcriptase. The ddPCR and idPCR reverse transcription reactions were primed with 100 ng 3' anchored-oligo d(T) primer (5'-ATGAATTCTTTTTTTTTTTTA/C/GN-3') or 250 ng of 3'-hp primer (5'-TAGAATTCCGTNCGTAGTTCTTGAGAACCA-3') (N represents A, C, G or T), respectively, and incubated for 1 hour at 42°C or 50°C respectively followed by incubation at 94°C for 5 minutes.
1-2 µl of cDNA was used as template in 25 µl PCR reactions containing 1.5 mM MgCl2, 1x Taq buffer (Geneworks), 20 µM each dNTP, 37.5 ng arbitrary tenmer (Operon technologies), 5 µCi [-33P] dATP, 1 U Taq polymerase (Geneworks) and 100 ng of 3'-anchored-oligo d(T) primer (ddPCR) or 500 ng 3'-hp (idPCR). PCR reactions were amplified in a MJ Research PTC-100 thermal cycler for 40 cycles of 94°C for 1 minute, 42°C for 1 minute, 72°C for 30 seconds (ddPCR) or 40 cycles of 94°C for 30 seconds, 42°C for 30 seconds, 72°C for 30 seconds (idPCR), followed by a final extension at 72°C for 5 minutes. PCR products were separated by electrophoresis on 6% denaturing polyacrylamide gels and exposed to X-ray film for 1-3 days.
Selected PCR products were excised from the gel and eluted in 100 µl of 0.5 M ammonium acetate, 10 mM magnesium acetate, 1 mM EDTA pH 8.0, 0.1% SDS at 50°C for 5-20 hours. 1 µl of eluted ddPCR product was used directly as a template in 100 µl PCR reamplification reactions under the same conditions as the original ddPCR or idPCR, omitting radioactive label. Reamplified PCR products were cloned into pBluescript KS and sequenced using T7 and T3 primers, using a T7 DNA sequencing kit (Pharmacia).
Use of the more complex reverse transcription primer for idPCR decreased the number of products amplified with each primer combination but reduced the background of spurious amplification products thereby facilitating reamplification and cloning of specific differently expressed products.
RNA isolation and northern blot analysis
Cytoplasmic RNA was isolated from cultured cells using the method of Edwards et al. (Edwards et al., 1985). Poly-A+ RNA was isolated using the method of Celano et al. (Celano et al., 1993
). Northern blot analysis was carried out as described in Thomas et al. (Thomas et al., 1995
) using 25 µg total RNA or 4 µg poly-A+ RNA. DNA probes were prepared from DNA fragments using a Gigaprime labelling kit (Geneworks). Fragments for CRTR-1 (736 bp), Psc1 (458bp) and PRCE (232 bp) were excised from pBluescript KS II clones by XhoI-BamHI, BamHI-HindIII and BamHI-HindIII digestion, respectively. Rex1, Fgf5, Oct4 and mGAP probes were as described in Rathjen et al. (Rathjen et al., 1999
). Band intensity was determined by volume integration using ImageQuant (Molecular Dynamics)
In situ hybridization
Wholemount in situ hybridization of both cell layers and embryos was carried out using the method of Rosen and Beddington (Rosen and Beddington, 1993) with the following modifications. Cell layers and 3.5-5.5 d.p.c. embryos were washed in RIPA buffer three times for 20 minutes before post-fixing. Embryos were prehybridized, hybridized and washed at 63.5°C for Psc1, 65°C for Oct4, Rex1, CRTR-1 and PRCE and 68°C for Fgf5. The conjugate of anti-digoxygenin Fab with alkaline phosphatase was not preabsorbed with embryo powder prior to use in cell layer in situ hybridization. Sense and antisense CRTR-1 digoxygenin riboprobes were generated from the pBluescript II KS clone by BamHI digestion and transcription with T7 RNA polymerase, and by XhoI digestion and transcription with T3 RNA polymerase, respectively. Sense and antisense Psc1 digoxygenin-labelled-riboprobes were generated from the pBluescript II KS clone by digestion with HindIII and BamHI, respectively, and transcription with T3 and T7 RNA polymerase, respectively. PRCE sense and antisense digoxygenin riboprobes were generated from the pBluescript KS II clone by BamHI digestion and transcription with T3 RNA polymerase, and by HindIII digestion and transcription with T7 RNA polymerase, respectively. Rex1 sense and antisense probes were produced from an 848 bp clone in pCRTMII (Hosler et al., 1989
) (obtained from N. Clarke) by digestion with BamHI and XbaI, respectively, and transcription with T7 and Sp6 RNA polymerases, respectively. Oct4 and Fgf5 probes were generated as described in Rathjen et al. (Rathjen et al., 1999
).
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Results |
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Poly-A+ RNA, isolated from ES and EPL cells cultured in 50% MEDII in the presence or absence of exogenous LIF for 2, 4 and 6 days was analysed by northern blot for expression of Oct4, CRTR-1, Rex1, Fgf5 and Psc1 (Fig. 2A). PRCE expression was confirmed by northern blot of total RNA from ES and EPL cells after 2, 4, 6 and 8 days of culture in 50% MEDII in the presence of LIF (Fig. 2B). Oct4 was expressed at high levels in ES and EPL cell cultures as described previously (Rathjen et al., 1999), consistent with the expression of this gene in ICM and primitive ectoderm cells in vivo (Rosner et al., 1990
; Scholer et al., 1990
; Yeom et al., 1991
). A 9.4 kb CRTR-1 transcript was produced in ES and EPL cells cultured for 2 days in MEDII or 2 and 4 days in MEDII + LIF, and was downregulated with EPL cell culture to undetectable levels after culture for 4 days in the absence of LIF or 6 days in the presence of LIF. Rex1 was also expressed at highest levels in ES cells and downregulated during EPL cell culture, with kinetics indistinguishable from those of CRTR-1. A 5.5 kb Psc1 transcript was detected in ES and EPL cells cultured for 2 days in the absence of LIF, or for 2 and 4 days in the presence of LIF. In comparison with both CRTR-1 and Rex1, expression of this gene persisted longer in EPL cells, with low but detectable levels of expression in EPL cells cultured for 4 days in the absence of LIF and downregulation to very low levels only being observed on day 6 in both the presence and absence of LIF. Fgf5 expression could not be detected in ES cells but was upregulated in EPL cells cultured for 4 days in the presence and 2 days in the absence of LIF, with a further increase in expression after culture of EPL cells for 6 and 4 days, respectively. A 6.6 kb PRCE transcript was detected in ES and EPL cells. Expression was upregulated upon formation of EPL cells, with a peak of expression 1.7 times that seen in ES cells seen after culture for 4 days in 50% MEDII + LIF (Fig. 2B). Further culture in 50% MEDII + LIF resulted in a downregulation of PRCE expression. In situ hybridization of ES and EPL cells with antisense digoxygenin-labelled probes specific for PRCE and Oct4 confirmed the upregulation of PRCE expression between ES and EPL cells cultured for 2 days (Fig. 2C).
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ES and EPL cell cultures can be contaminated by low levels of differentiated cells (Rathjen et al., 1999), generally located at the periphery of colonies. Expression of CRTR-1, Psc1 and PRCE was shown to be associated with pluripotent cells by in situ hybridization to ES and EPL cell monolayers in vitro using antisense digoxygenin-labelled probes. Expression of these genes was associated with most cells in the culture, located centrally within colonies and exhibiting a pluripotent cell morphology (Rathjen et al., 1999
). Sporadic differentiated cells within the cultures did not express CRTR-1, Psc1 or PRCE (Fig. 2D; data not shown).
Sequence of cDNAs
The CRTR-1, Psc1 and PRCE cDNA fragments in pBluescript were sequenced in both directions, revealing sizes of 736 bp, 458 bp and 232 bp, respectively. The CRTR-1 and PRCE cDNAs were isolated using a 3'-anchored-oligo (dT) primer and potentially represent 3' UTR sequence in these large transcripts. Further analysis of CRTR-1 cDNA and the biochemical properties of CRTR-1 has revealed homology to the CP2 family of transcription factors (Rodda et al., 2001) (accession number AF311309). Analysis of PRCE revealed 71.3% identity with the human cDNA KIAA0165 (Fig. 3A), isolated from the immature myeloid cell line KG-1 (Nagase et al., 1996
). Additional sequence data and analysis suggest functional equivalence with fungal separins and a role in chromosome segregation (T. A. Pelton, PhD thesis, Adelaide University, 2000). The Psc1 cDNA fragment, which was isolated by idPCR, contained an uninterrupted open reading frame with a region homologous (44.8% identity) to the predicted CELB0336.3 open reading frame from Caenorhabditis elegans (Wilson et al., 1994
) (accession number U32305; Fig. 3B). Sequence homologies and immunocytochemical analysis of protein localization in transfected cells indicate that Psc1 is a member of the SR protein family with a role in RNA metabolism (S. J. Kavanagh, Honours thesis, Adelaide University, 1998).
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In vivo expression of Psc1, CRTR-1, PRCE, Rex1 and Fgf5
Flushed pre- and early post-implantation embryos (to 4.75 d.p.c.) or dissected post-implantation embryos were examined for gene expression by whole mount in situ hybridization. To allow detailed mapping of expression during this experimentally difficult period, embryos were isolated at
0.25 d.p.c. intervals.
CRTR-1 expression (Fig. 5A) was detected uniformly in pluripotent cells of the 3.5 d.p.c. embryo and 4.5 d.p.c. expanded blastocyst. Expression was inconsistent and at low levels in 4.75 d.p.c. embryos, suggesting downregulation of the gene at this time. CRTR-1 transcripts were not detected in pluripotent cell populations of the 5.0 d.p.c. or 5.25 d.p.c. embryo, or in extra-embryonic cell types from the trophectodermal or primitive endoderm lineages at any of the stages analysed.
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Psc1 was found to be expressed within the pluripotent cell pool in a pattern overlapping but distinct from those of CRTR-1 and Rex1 (Fig. 5C). Early embryonic expression was observed in all pluripotent cells but not trophectodermal cells of the 3.5 d.p.c. blastocyst and was maintained in the compacted epiblast of the expanded blastocyst at 4.5 d.p.c. In contrast to CRTR-1 and Rex1, Psc1 transcripts were detected uniformly throughout cells of the epiblast at 4.75 d.p.c. and at lower, variable levels in pluripotent cells at 5.0 d.p.c., immediately prior to proamniotic cavity formation. Expression was downregulated in pluripotent cells immediately following the earliest morphological evidence of proamniotic cavitation, a later stage than downregulation of CRTR-1 and Rex1, and was not observed in later stage embryos that contained larger proamniotic cavities. Psc1 expression was not detected in primitive endoderm or other extra-embryonic cell types.
Expression of PRCE was not detected by whole mount in situ hybridization in 3.5 d.p.c. (not shown) and 4.5 d.p.c. embryos (Fig. 6A). Low level expression was first detected throughout the pluripotent cell pool at 4.75 d.p.c. and higher levels of expression were apparent in pluripotent cells of 5.0 d.p.c. embryos. PRCE expression persisted within pluripotent cells at 5.25 d.p.c., after formation of the proamniotic cavity. Expression in the extra-embryonic ectoderm at 5.25 d.p.c. was also noted. PRCE expression was not detected following polarization and reorganization of primitive ectoderm into a columnar epithelial sheet at 5.5-5.75 d.p.c.
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Discussion |
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Expression of Psc1 in the absence of CRTR-1 and Rex1 identified a subsequent pluripotent cell population present between 4.75 d.p.c. and the onset of proamniotic cavitation. Downregulation of Psc1 expression between 5.0 d.p.c. and 5.25 d.p.c., and expression of Fgf5 resulted in a population of pluripotent cells that expressed Oct4, PRCE and Fgf5, and was succeeded, following downregulation of PRCE expression between 5.25 d.p.c. and 5.5 d.p.c., by a pluripotent cell population expressing Oct4 and Fgf5. This expression pattern is maintained in pluripotent cells until gastrulation (Haub and Goldfarb, 1991; Yeom et al., 1991
).
The existence of temporal intermediate pluripotent cell populations in vivo, which differ from preceding and following pluripotent cell populations, can therefore be demonstrated by different gene expression. This is perhaps best illustrated by the expression of PRCE, which was expressed in pluripotent cells in a brief developmental window between 4.75 d.p.c. and 5.25 d.p.c. Whereas pluripotent cell populations exhibited the anticipated temporal heterogeneity in gene expression, no evidence was obtained for spatial heterogeneity within the pluripotent cell pool. This is consistent with the demonstration that spatially distinct pluripotent cells in 5.5 d.p.c. and 6.5 d.p.c. embryos are not committed to alternate developmental fates (Beddington, 1981; Beddington, 1982
; Beddington, 1983b
; Lawson et al., 1991
).
Alterations in pluripotent cell gene expression correlate with developmental events
The delineation of temporally distinct pluripotent cell populations in vivo points to a gradual transition between the ICM and primitive ectoderm. This stage of mouse embryogenesis is characterized by morphologically identifiable developmental events that correlate closely with the alterations in gene expression. A major change in pluripotent cell gene expression was mapped at 4.75 d.p.c., when CRTR-1 and Rex1 were downregulated, apparently simultaneously, and PRCE expression was initiated. This coincides with an embryological transition from slow cell division within the oviduct with no overall increase in embryo size to a rapid increase in the growth rate of the embryo, particularly within the pluripotent cells between 4.5 d.p.c. and 5.5 d.p.c. (Snow, 1977
; Hogan et al., 1994
). A number of null mutant embryos, including Fgf4 (Feldman et al., 1995
), Fgfr2 (Armand et al., 1998
), Brca1 (Hakem et al., 1996
; Liu et al., 1996
; Ludwig et al., 1997
), ß1 integrin (Fassler and Meyer, 1995
; Stephens et al., 1995
) and EGFR (Threadgill et al., 1995
), degenerate following implantation as a result of impaired pluripotent cell proliferation. Different phenotypes suggest the involvement of several signalling pathways at this time. Alterations in Rex1, CRTR-1 and PRCE expression may be responsive to extracellular signals regulating this phase of peri-implantation pluripotent cell development, such as members of the FGF family (Feldmen et al., 1995
; Armand et al., 1998
). The unexpected expression of Fgf5 in primitive endoderm at 4.75 d.p.c., as reported in this work, provides a potential source of these signals, consistent with a hypothesized instructive role for this extra-embryonic cell type in the blastocyst (Thomas et al., 1998
).
In similar manner, downregulation of Psc1 expression coincided with formation of the proamniotic cavity, a process thought to be regulated by the combined action of a diffusible death signal secreted by visceral endoderm and a matrix-associated survival signal (Coucouvanis and Martin, 1995) and downregulation of PRCE expression correlated with reorganization of pluripotent cells into a sheet of pseudostratified epithelium in vivo. Transcriptional analysis of differently expressed pluripotent cell markers may therefore provide a means for identification of the cellular origin and timing of important embryonic signals.
Identification of pluripotent cell populations in vitro
Gene expression specific for ES cells, as assessed by expression of Oct4, CRTR-1, Rex1 and Psc1, and lack of Fgf5, was consistent with gene expression in pluripotent cells in vivo between 3.5 d.p.c. and 4.75 d.p.c. Although early results have commonly been interpreted as suggesting an embryonic origin for ES cells in pre-implantation embryos, it has recently been demonstrated that ES cell lines can be isolated from peri-implantation embryos with greater efficiency (Brook and Gardner, 1997). Although our results are consistent with either an early or a late embryonic equivalent to ES cells, the gene expression pattern described in this work provides a latest time point for the embryonic equivalent at
4.5 d.p.c. This is consistent with the demonstration that pluripotent cells from post-implantation embryos do not contribute to chimaeras following blastocyst injection (Beddington, 1983a
). Expression of genes such as Rex1 and CRTR-1, if conserved across species, could provide superior markers for the identification of ES cell equivalents in the embryos of diverse species.
Although expression of the PRCE gene was tightly regulated in vivo, PRCE expression in vitro appeared to be less tightly regulated with low level expression detected in ES cells by ddPCR, northern blot and in situ hybridization. Co-expression in ES cells of Rex1 and CRTR-1 with PRCE is inconsistent with our descriptions of pluripotent cells in vivo and may be associated with the rapid proliferation exhibited by ES cells in culture and pluripotent cells of the peri-implantation embryo or simply reflect deregulated gene expression associated with growth in vitro.
Although there is some variability in the rate of ES-EPL cell transition, the temporal regulation of gene expression described in pluripotent cells in vivo was recapitulated in this in vitro system, supporting our previous identification of late EPL cells as representative of early primitive ectoderm (Rathjen et al., 1999). EPL cells cultured for 4 days in the presence of LIF or 2 days in the absence of LIF demonstrated a gene expression pattern analogous to pluripotent cells of the 4.75 d.p.c. embryo in which expression of CRTR-1 and Rex1 was reduced and PRCE increased. EPL cells cultured for 4 days in the absence or 6 days in the presence of LIF shared gene expression with pluripotent cells of the 4.75-5.0 d.p.c. embryo, which express Psc1 and PRCE but not CRTR-1 or Rex1. EPL cells cultured for 6 days in the absence of LIF demonstrated downregulated Psc1 expression but retained high level expression of Fgf5 and therefore expressed a repertoire of genes analogous to primitive ectoderm populations occurring
5.25 d.p.c. Downregulation of PRCE expression was slow in vitro, in contrast to the tight regulation of this gene between 4.75 d.p.c. and 5.25 d.p.c. in vivo. This may reflect the lack in vitro of three-dimensional architecture required for epithelialization. In general, the developmental progression in vitro was significantly slower than that observed in vivo. This is common to many cell culture systems and presumably reflects absence of an optimal environment for developmental progression in vitro.
Analysis of genes expressed differently between ES and EPL cells therefore allowed the definitive identification of previously unidentified pluripotent cell populations. These populations may share properties with some of the diverse pluripotent cell lines that have been isolated from non-mouse mammalian species (Thomson et al., 1998; Reubinoff et al., 2000
) and non-ICM pluripotent cell sources (Pera, 1990
; Resnick et al., 1992
; Matsui et al., 1992
; Shamblott et al., 1998
). Molecular characterization of these populations with Psc1, CRTR-1 and PRCE allowed a close alignment of in vivo populations and EPL cells, and demonstrated that, after 6-8 days in culture, EPL cells share a gene expression profile with 5.25 d.p.c. primitive ectoderm. EPL cells cultured for less than 6-8 days could be aligned with temporal pluripotent intermediates formed in the embryo between 4.5 d.p.c. and 5.25 d.p.c. The ability to generate a homogeneous population in culture equivalent to primitive ectoderm provides a substrate for the development of model systems for the investigation of pluripotent cell differentiation, and may provide a starting point for the development of procedures for directed cell differentiation and production of cell populations with therapeutic applications from pluripotent cells.
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ACKNOWLEDGMENTS |
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