Association of the tetraspanin CD151 with the laminin-binding integrins {alpha}3ß1, {alpha}6ß1, {alpha}6ß4 and {alpha}7ß1 in cells in culture and in vivo

Sterk, L. M. T., Geuijen, C. A. W., van den Berg, J. G., Claessen, N., Weening, J. J. and Sonnenberg, A. J. Cell Sci. 115, 1161-1173.

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Fig. 10. Expression of laminin-binding integrins on transfected K562 cells increases CD151 cell surface expression. Flow cytometric analysis of wild-type K562 cells endogenously expressing {alpha}5ß1 and transfected K562 also expressing {alpha}vß1, {alpha}3ß1, {alpha}6ß1, {alpha}6ß4, {alpha}7X1ß1 or {alpha}7X2ß1. Solid lines in the left panels indicate staining with anti-integrin antibodies: anti-{alpha}5 mAb Sam-1 (K562 cells), anti-{alpha}v mAb 13C2 (K562-{alpha}v), anti-{alpha}3 mAb J143 (K562-{alpha}3), anti-{alpha}6 mAb GoH3 (K562-{alpha}6), anti-ß4 mAb 439-9B (K562-{alpha}6ß4) and anti-{alpha}7 mAb CA25 (K562-{alpha}7BX1 and K562-{alpha}7BX2). Staining of cells with anti-CD151 antibodies is shown in the right panels. Solid lines represent staining of cells with mAb TS151R and broken lines with mAb P48. A negative control (dotted line) with secondary goat anti-mouse or rat IgG alone is shown in each panel. R represents the ratio of the mean fluorescence intensity of cells stained with mAb P48 and with secondary antibody alone. A three- to sixfold increase of CD151 expression is observed in transfected cells that ectopically express lamininbinding integrins. The TS151R epitope is masked in all cells expressing one of the laminin-binding integrins.

 





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