Correspondence to Alan Hall: alan.hall{at}ucl.ac.uk
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Introduction |
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The formation of neuronal branches depends on the ability of the cell cortex and cytoplasm of elongating neurites to undergo dynamic reorganization in response to extrinsic cues. The sprouting of new motile structures, such as filopodia and lamellae, is thought to be an early step in this process (Gallo and Letourneau, 2004). A variety of extracellular cues, such as neurotrophic factors, slits, ephrins, netrins, semaphorins, integrins, and cell adhesion molecules, can affect neurite branching (Acebes and Ferrus, 2000; Dent et al., 2004). The signals involved in establishing a branch point, however, are poorly understood. It is likely that neurite branching requires the coordination of multiple events, including actin polymerization, formation of new adhesive sites, changes to the microtubule cytoskeleton, and membrane delivery.
The two Ral isoforms, RalA and -B, were originally associated with growth factorinduced Ras signaling pathways and are activated by a family of Ral guanine nucleotide exchange factors (GEFs), which includes Ral GDP dissociation stimulator, Rgl1, Rgl2, and Rlf (Quilliam et al., 2002). RalA and -B are found at the plasma membrane as well as on endocytic and exocytic vesicles, and they have been implicated in a variety of cellular processes, such as oncogenic transformation, transcriptional regulation, cell proliferation, vesicle trafficking, and cytoskeletal reorganization (Feig, 2003). Several effector molecules connect Ral with endocytic/exocytic pathways and cytoskeletal regulation. RalBP1, a Rac and Cdc42 GTPase-activating protein, also known as RLIP76, interacts with GTP-bound Ral and binds to proteins involved in clathrin-mediated endocytosis (Jullien-Flores et al., 2000). In addition, the association of Ral with phospholipase D (PLD) may play a role both in Golgi vesicle formation and in receptor-mediated endocytosis (Shen et al., 2001). Finally, the identification of the exocyst complex as a Ral effector has implicated this GTPase not only in the regulation of polarized membrane delivery (Moskalenko et al., 2002; Shipitsin and Feig, 2004) but also in Cdc42-dependent filopodia formation (Sugihara et al., 2002), suggesting that Ral has a role in the modulation of the actin cytoskeleton.
In neuronal cells, Ral has been found to be associated with cholinergic synaptic vesicles (Ngsee et al., 1991) and is involved in neurosecretion in PC12 cells (Moskalenko et al., 2002). Refilling of the readily releasable pool of synaptic vesicles is suppressed in transgenic mice expressing a dominant inhibitory form of RalA (Polzin et al., 2002). In addition, Ral associates with PLD2 and class 1 metabotropic glutamate receptors 1a and 5a in the adult rat brain and may regulate their constitutive endocytosis (Bhattacharya et al., 2004). Finally, Ral activation has been shown to delay NGF-induced neurite outgrowth in PC12 cells (Goi et al., 1999), but the connection between Ral and cytoskeletal remodeling in neurons has yet to be determined.
We report that Ral regulates neurite branching in cortical and sympathetic neurons. Activation of Ral leads to increased branching, whereas inhibition blocks integrin-mediated neurite branching. RalA and -B promote branching through two different pathways involving the exocyst complex and PLD, and the latter leads to PKC-dependent phosphorylation of 43-kD growth-associated protein (GAP-43), which is implicated in branching and axonal regeneration (Oestreicher et al., 1997; Bomze et al., 2001).
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Results |
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Ral is required for laminin-induced neurite branching
Because integrin activation has been implicated in the regulation of neurite outgrowth and branching (Ivins et al., 2000), we tested the influence of different substrates on the levels of Ral activation. SCG neurons were plated on plastic dishes coated with polyornithine only or polyornithine plus either laminin or collagen. 3 h after plating, neuronal morphology varied according to the substrate (Fig. 3 A). SCGs plated on collagen or on polyornithine appeared mainly round with very short processes (Fig. 3 A, top left and bottom). In contrast, cells growing on laminin had longer, branched neurites (Fig. 3 A, top right). To quantify the levels of active Ral under these different conditions, we used a fragment of RalBP1 known to preferentially bind to Ral-GTP as an affinity reagent (Tian et al., 2002). Ral-GTP levels in neurons plated on laminin were approximately fourfold higher than on the other substrates, whereas the total amount of Ral in lysates from different samples was comparable (Fig. 3 B).
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Cytoskeletal characterization of Ral-induced neurite branches
Neurite branching requires reorganization of the cytoskeleton and is generally initiated by the appearance of motile filopodia in response to extracellular cues (Gallo and Letourneau, 2004). We stained the Ral-induced branches with fluorescent phalloidin to visualize actin filaments and an anti-tubulin antibody to reveal microtubules. In SCG neurons expressing Rlf-CAAX, phalloidin labeled filopodia, short nascent branches, and long branches, but microtubules could be detected only in longer branches (Fig. 4, A and B). Notably, Rlf-CAAX appeared to be concentrated in puncta along neurites and sprouting branches and was often present at branch points (Fig. 4 B, arrowheads). SCG neurons expressing constitutively active Ral showed clear filopodial sprouting also from cell bodies (Fig. S2, available at http://www.jcb.org/cgi/content/full/jcb.200507061/DC1). Treatment of neurons with cytochalasin D, an inhibitor of actin polymerization, blocked filopodial sprouting and branching in a dose-dependent manner (Fig. 4 C). These observations suggest that active Ral causes branching by promoting the formation of filopodia and then allowing microtubules to invade the branch, consistent with previous studies on branch initiation (Dent et al., 2003; Gallo and Letourneau, 2004).
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Discussion |
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We show that both RalA and -B promote phosphorylation of GAP-43 but only RalA acts through the exocyst complex. These results indicate that the two Ral isoforms may promote branching by different mechanisms; in support of this, expression of Rlf-CAAX, a Ral-GEF able to activate both endogenous RalA and -B, produced the most striking effect on branching. In addition, knocking down a single Ral isoform by RNAi produced a significant decrease in branching, indicating that RalA and -B may play distinct roles in regulating branch number. RalA has been reported to bind to the exocyst complex more effectively than active RalB both in vitro and in cultured cells (Shipitsin and Feig, 2004). Even though the two Ral isoforms are 85% identical, they differ in their COOH-terminal domain, which is responsible for their distinct cellular localization, as shown in MDCK cells (Shipitsin and Feig, 2004). It is possible that the different localization of Ral isoforms could differentially modulate their interaction with various effectors.
Disrupting the interaction of RalA with the exocyst impaired the ability of active RalA to promote branching, implying that the exocyst plays an important role in branching. Previous papers have implicated the exocyst, a complex consisting of at least eight subunits, in cytoskeletal regulation. Interaction between RalA and the exocyst complex has been shown to mediate Cdc42-dependent filopodia formation in fibroblasts (Sugihara et al., 2002). PC12 cells expressing dominant-negative sec10 and ventral cord neurons from sec5 mutant Drosophila melanogaster larvae fail to extend neurites (Vega and Hsu, 2001; Murthy et al., 2003). Interestingly, sec5 larvae also show growth arrest of neuromuscular junctions and impaired motor neuron arborizations on muscles, suggesting a role for the exocyst complex in regulating the normal development of terminal innervation sites (Murthy et al., 2003). Neurite branching is characterized by the splaying apart and fragmentation of microtubules and by filopodial activity (Gallo and Letourneau, 2004), events that we observe upon Ral activation. Interestingly, local destabilization of microtubules along axons can promote plasma membrane addition, and the exocyst complex has been shown to inhibit tubulin polymerization in vitro (Wang et al., 2004). In normal rat kidney cells, overexpression of the exocyst subunit exo70 results in disruption of the microtubule network and increased plasma membrane addition in the form of long and thin plasma membrane protrusions (Wang et al., 2004). Thus, recruitment of the exocyst complex by active Ral could promote branching through a dual mechanism involving the coordinated regulation of microtubule dynamics and membrane delivery.
Our data indicate that the association between Ral and PLD plays a crucial role in promoting branching, particularly for RalB. Although Ral has no effect on the activity of PLD in vitro, activation of PLD by v-Src, for example, is Ral dependent (Jiang et al., 1995; Luo et al., 1998). Moreover, interaction of Ral with PLD synergistically enhances Arf6-dependent PLD activation downstream of Ras (Xu et al., 2003), and Arf6 has been implicated in the regulation of both axonal and dendritic branching (Hernandez-Deviez et al., 2004) and in the efficient recycling of ß1 integrin (Brown et al., 2001). In addition, GTP-bound Arf6 can promote membrane recycling toward specialized plasma membrane regions by directly interacting with the exocyst subunit Sec10 (Prigent et al., 2003). Arf6 activity has also been consistently associated with the formation of actin-based plasma membrane protrusions (Prigent et al., 2003). Although the existence of a signaling pathway downstream of integrins involving Ral and Arf6 remains to be demonstrated, these intriguing links further point to Ral as an attractive candidate molecule likely to coordinate cytoskeletal reorganization and polarized endocytic recycling in response to extracellular matrix stimuli.
PLD generates phosphatidic acid, a multifunctional lipid that can be further metabolized to other important signaling lipids, such as DAG, which is a PKC activator, and lysophosphatidic acid. Several in vitro studies have suggested that PLD plays a role in neurite outgrowth. PLD expression and activity increase during neuronal differentiation of PC12 cells and the immortalized mouse hippocampal stem cell line HiB5 (Min et al., 2001; Sung et al., 2001). In addition, inhibiting PLD activity impairs L1-stimulated neurite outgrowth in cerebellar granule neurons (Watanabe et al., 2004). Interestingly, PLD1 increasingly associates with PKC- and -ßII during NGF-induced neuronal differentiation of PC12 cells (Min et al., 2001), suggesting that PLD and PKC activities are correlated to induce differentiation. Active Ral might then serve a dual function by recruiting PLD to the membrane and enhancing its activity, leading to PKC activation and subsequent phosphorylation of substrates such as GAP-43. In agreement with this, in models of experimentally induced sprouting such as kainate-induced outgrowth of hippocampal mossy fibers, both PLD and GAP-43 are strongly reexpressed (Zhang et al., 2004). Sprouting responses are usually triggered after injury by the release of diffusible growth factors and the up-regulation of several extracellular matrix molecules, among them laminins, which facilitate adhesion and outgrowth of the sprouting processes. Ral could therefore play an important role in coupling extracellular matrix signals to the cytoskeleton via GAP-43 and to vesicle trafficking via the exocyst complex, ultimately leading to branching.
We show that Ral regulates branching through phosphorylation of GAP-43. GAP-43 is generally considered an intrinsic determinant for neurite outgrowth and plasticity. It is maximally expressed during nervous system development and reinduced in injured and regenerating neural tissues, and it serves to potentiate growth cone and nerve terminal responses to local growth and guidance signals. Its expression is not an absolute requirement for neurite outgrowth either in vitro or in vivo (Aigner and Caroni, 1995; Strittmatter et al., 1995). However, depletion experiments in cultured neurons indicate that GAP-43 greatly promotes adhesion and persistent growth cone spreading and branching (Aigner and Caroni, 1995). Membrane anchoring of GAP-43 through palmitoylation plays a crucial role for its morphogenic effects, possibly promoting its recruitment to specialized membrane microdomains enriched in sphingolipids. Incorporation of a lipidated motif such as the one present in GAP-43 into lipid rafts may create local changes in membrane tension and the extension of filopodial structures (Gauthier-Campbell et al., 2004). We found that overexpressed active Ral (particularly RalB) colocalizes in patches along neurites with endogenous GAP-43 (unpublished data). These patches might represent functional adhesion sites, as GAP-43 has been shown to associate with areas of membrane that are tightly bound to the substrate and belong to the membrane cytoskeletal fraction (Meiri and Gordon-Weeks, 1990). Extracellular matrixinduced localization of integrins in lipid rafts can help generate and maintain a specific signaling environment (Decker et al., 2004). Activation of Ral by integrin stimulation along neurites might help recruit important branch-promoting effectors, such as the exocyst complex and PLD, to such signaling microdomains.
We found that Ral activation increases phosphorylation of endogenous GAP-43 in cortical neurons. Interestingly, PKC-mediated phosphorylation of GAP-43 correlates with increased stabilization of the interaction between growth cones and substrate and, conversely, unphosphorylated GAP-43 is always found in retracting or collapsing areas (Dent and Meiri, 1992). In addition, NGF-treated PC12 cells lacking endogenous GAP-43 and expressing nonphosphorylatable GAP-43 display abnormalities in long-term adhesion to laminin substrates, surface expression of integrins, and cell morphology, with marked membrane blebbing and varicosities (Meiri et al., 1996). Phosphorylation of GAP-43 by PKC therefore seems to maintain a functional coupling between the plasma membrane and the underlying cytoskeleton. Indeed, phosphorylated GAP-43 can stabilize long actin filaments in vitro (He et al., 1997) and can enhance the accumulation of phosphatidylinositol-4,5-bisphosphaterich plasmalemmal patches, which are possible sites of signal-induced actin assembly (Laux et al., 2000). Maintenance of phosphorylated GAP-43 induced by local Ral activation might therefore contribute to the stabilization of signaling pathways promoting actin polymerization and, ultimately, branching.
GAP-43 knockout mice display pathfinding defects and perturbed development of cortical maps (Strittmatter et al., 1995; Maier et al., 1999), whereas mice overexpressing GAP-43 show striking spontaneous and lesion-induced nerve sprouting, with highly potentiated terminal arborization during innervation (Aigner et al., 1995). Interestingly, several extracellular matrix molecules are up-regulated after injury to either the peripheral or the central nervous system, among them both laminins and collagens (Ivins et al., 2000). In this context, and given that exposure to laminin activates Ral, it is tempting to speculate that this GTPase could act as a mediator between integrin signaling and GAP-43 to potentiate lesion-induced sprouting. Integrins are critical determinants of basic morphogenesis for many aspects of embryogenesis, including nervous system development. Indeed, they participate in a variety of processes, such as brain lamination, neuroblast migration, and axon navigation and synaptogenesis (Milner and Campbell, 2002). Our data point to Ral as an important molecular link between integrin-dependent signaling and cytoskeletal reorganization in neurons. Further investigation should help clarify the involvement of Ral in axonal pathfinding and synapse formation, events relying on both integrin signaling and branch/filopodia formation.
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Materials and methods |
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Plasmids
All Ral mutant constructs were made by PCR or restriction digestions using standard procedures. RalA28N and -72L were cloned into pRK5-myc for expression in mammalian cells, RalB28N and -23V were amplified by PCR using pBabepuroRalB28N and -23V as templates (a gift from K. Kelly, National Cancer Institute, Bethesda, MD) and the following primers: 5'-tcggatccATGGCTGCCAACAAGAGTAAG-3' and 5'-agtctagaTCATAGTAAGCAACATCTTTC-3'. PCR fragments were then cloned into the BamHI and XbaI sites of pRK5myc. RalA72L and -B23V mutants were constructed from pRK5-myc-RalA72L and pRK5-myc-RalB23V using the Quickchange mutagenesis kit (Stratagene) and the following PCR primers: 5'-GGAGGACCTGGGATCCTTGGCCTTACACAAAG-3' and 5'-CTTTGTGTAAGGCCAAGGATCCCAGGTCCTCC-3' for pRK5-myc-RalA72LN11, 5'-GGAGGACCTGGGATCCTTGGCCCTCCACAAGG-3' and 5'-CCTTGTGGAGGGCCAAGGATCCCAGGTCCTCC-3' for pRK5-myc-RalB23V
N11, 5'-GCCTACCAAAGCAGAAAGCTACCGGAAGAAGG-3' and 5'-CCTTCTTCCGGTAGCTTTCTGCTTTGGTAGGC-3' for pRK5-myc-RalA72LD49E, 5'-GCCTACCAAAGCAAACAGCTACCGGAAGAAGG-3' and 5'-CCTTCTTCCGGTAGCTGTTTGCTTTGGTAGGC-3' for pRK5-myc-RalA72LD49N, 5'-CCTACCAAAGCTGAAAGTTATAGAAAGAAAGTGG-3' and 5'-CCACTTTCTTTCTATAACTTTCAGCTTTGGTAGG-3' for pRK5-myc-RalB23VD49E, and 5'-CCTACCAAAGCTAACAGTTATAGAAAGAAAGTGG-3' and 5'-CCACTTTCTTTCTATAACTGTTAGCTTTGGTAGG-3' for pRK5-myc-RalB23VD49N. pMT2-HA-Rlf-CAAX and pMT2-HA-Rlf-
CAT-CAAX were a gift from J. Bos (University of Utrecht, Utrecht, Netherlands). pcDNA3cGAP-43 (wild type), pcDNA3cGAP-43(S42A), and pcDNA3cGAP-43(S42D) were provided by P. Caroni. pGEX-2TK-RalBP1-RBD was a gift from L. Feig (Tufts University, Boston, MA). pEGFP-F was obtained from CLONTECH Laboratories, Inc.
Cell culture
COS-7 cells were maintained in DME supplemented with 10% FCS, 100 IU/ml penicillin, and 100 µg/ml streptomycin and incubated at 37°C and 10% CO2. Cells were transfected using GeneJuice (Novagen) according to the manufacturer's instructions. Sympathetic neurons were isolated from SCG of newborn Sprague-Dawley rats. Cells were plated on 35-mm plastic dishes or 12-mm glass coverslips coated with 150 µg/ml polyornithine and 10 µg/ml laminin at a density of 30,000 cells/coverslip for immunofluorescence and at least 200,000 cells/dish for Ral-GTP pull-down experiments. For experiments on different substrates, plastic dishes were coated with polyornithine only or with polyornithine and 100 µg/cm2 collagen. Neurons were maintained at 37°C at 5% CO2 in L-15 medium plus 5% FCS, 2 mM glutamine, 24 mM NaHCO3, 100 U/ml penicillin, 50 U/ml streptomycin, 38 mM glucose, 5 ng/ml 7S NGF (Alomone Labs), and 10 µM cytosine arabinoside to restrict glial cell growth. Brain cortices were dissected from E18 rat embryos and dissociated by incubation with 0.05% trypsin-EDTA (Invitrogen) for 20 min at 37°C followed by washing and trituration in DME plus 10% FCS. Dissociated neurons were nucleofected with a primary neuron nucleofection kit (Amaxa) according to the supplier's instructions. Cells were then plated on 35-mm plastic dishes or glass coverslips coated with polyornithine. Medium was replaced 23 h after nucleofection with neurobasal medium containing B27 supplement, 2 mM glutamine, and 35 mM glucose. In some experiments, 8 µM of the PKC inhibitor GF109203X or DMSO as control were added at the time of medium replacement and incubated overnight until preparation of neuronal lysates.
Microinjection and immunofluorescence
2 h after plating, constructs were injected at 0.1 µg/µl into the nucleus of cells over a period of 15 min and allowed to express overnight. In laminin-addition experiments, neurons were microinjected 2 h after plating only on polyornithine and left to express for at least 4 h before adding laminin to the medium (final concentration 50 µg/ml) to stimulate neurite outgrowth and branching. 24 h after microinjection, neurons were fixed in 3.7% paraformaldehyde and 20% sucrose in PBS for 15 min at room temperature. After rinsing twice in PBS, cells were incubated for 20 min with 50 mM NH4Cl in PBS, washed, blocked, and permeabilized with 0.1% Triton X-100, 2% BSA, 0.25% porcine skin gelatin, 0.2% glycine, and 15% FCS in PBS for 15 min at room temperature. Cells were then incubated for at least 1 h at room temperature with primary antibodies diluted in PBS containing 1% BSA and 0.25% porcine skin gelatin (antibody buffer). After rinsing, cells were incubated for 25 min with fluorescent secondary antibodies diluted 1:200 in antibody buffer. Coverslips were mounted on slides with fluorescence mounting medium (DakoCytomation). Fluorescent and phase-contrast images were captured with a charged-coupled device camera (C4742-95; Hamamatsu) connected either to an inverted microscope (DM IRB; Leica) equipped with a 10x 0.25 NA N-Plan objective or to a microscope (Axioskop; Carl Zeiss MicroImaging, Inc.) equipped with 10x 0.30 NA, 40x 1.30 NA, and 63x 1.25 NA Plan Neofluar objectives. Confocal pictures were acquired using a confocal system (MRC 1024; Bio-Rad Laboratories) with an ILT Krypton Argon laser connected to a microscope (Optiphot 2; Nikon) equipped with 40x 1.0 NA and 60x 1.40 NA Plan Apo objectives. Images were acquired using either Openlab 3.1.7 (Improvision) or Laser Sharp 2000 (Bio-Rad Laboratories) softwares and processed using Photoshop 6.0 (Adobe) or Metamorph 6.0 (Molecular Devices). In some cases, the brightness and contrast of whole images were adjusted to increase the visibility of neurites. Cell profiles were traced manually using Metamorph 6.0. Quantitative analysis of branching in SCG neurons was performed by counting all clearly visible neurite branches >10 µm long. The number of branches per neurite was then calculated by dividing the total number of branches by the number of main neurites (at least twice the length of the cell body diameter) extending from the cell body. For each construct or condition tested, between 30 and 180 neurons from three or more independent experiments were considered. Statistical analysis was performed using a Kruskal-Wallis nonparametric analysis of variance test and Dunn's post-hoc tests with InStat software 2.0 (GraphPad Software).
For RNAi experiments in cortical neurons, quantitative analysis of branching was performed by counting the number of branches positive for tubulin staining extending from the major neurite of those neurons with a clear polarized morphology. Statistical analysis was performed on at least 60 cells per condition from a total of four experiments.
RNAi
Freshly dissociated cortical neurons were nucleofected with 9 µg of siRNA duplexes targeting either the RalA sequence AGACTACGCTGCAATTAGA or the RalB sequence CGCTCCAGTTCATGTATGA (Dharmacon). As a control, a scramble siRNA duplex targeted against GFP (QIAGEN) was used. Cells were fixed 50 h after nucleofection and stained for RalA, RalB (1:50), and tubulin (1:200).
Measurement of GTP-bound state of endogenous Ral and GAP-43 phosphorylation levels
Neuronal lysates were analyzed for the presence of the active form of Ral (Ral-GTP) by affinity purification using a GST fusion with the Ral binding domain of RalBP1 (GSTRalBP1-RBD) immobilized on glutathione-agarose beads as described previously (Tian et al., 2002). To measure the phosphorylation levels of GAP-43, cells were washed twice on ice with cold PBS and scraped in lysis buffer (20 mM Tris-HCl, pH 7.6, 100 mM NaCl, 1% Triton X-100, 12 mM ß-glycerophosphate, 5 mM EGTA, 0.5% deoxycholate, 1 mM DTT, 10 mM NaF, 1 mM Na3VO4, and 1 mM PMSF). After adding Laemmli buffer, lysates were boiled, sonicated, and analyzed by SDS-PAGE and Western blotting. Immunoblots were visualized with anti-RalA or antiphospho-GAP-43 antibodies and ECL (GE Healthcare). Quantification by optical density was performed using Quantity One software (Bio-Rad Laboratories).
Online supplemental material
Fig. S1 shows a Western blot analysis of neurons nucleofected with Ral siRNA. Fig. S2 shows that active Ral causes filopodial sprouting from neuronal cell bodies. Fig. S3 shows that adhesion to laminin stimulates GAP-43 phosphorylation. Fig. S4 shows that Ral influences PKC-mediated GAP-43 phosphorylation. Online supplemental material is available at http://www.jcb.org/cgi/content/full/jcb.200507061/DC1.
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Acknowledgments |
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This work was supported by a Wellcome Trust project grant to A. Hall and G. Lalli (069534/z/02/z).
Submitted: 13 July 2005
Accepted: 31 October 2005
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References |
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