* Department of Surgery, University of Washington, Seattle, Washington 98195; Division of Clinical Research and
Molecular Medicine, and § Electron Microscope Laboratory, Shared Resources, Administrative Division, Fred Hutchinson
Cancer Research Center, Seattle, Washington 98195; and
Department of Surgery, Memorial Sloan Kettering Cancer Center,
New York 10021
Herbimycin A, a tyrosine kinase inhibitor,
induces cellular differentiation and delayed apoptosis
in Colo-205 cells, a poorly differentiated human colon
carcinoma cell line. Cell cycle analysis in conjunction
with end labeling of DNA fragments revealed that G2
arrest preceded apoptotic cell death. Ultrastructural examination of herbimycin-treated cells demonstrated
morphologic features of epithelial differentiation, including formation of a microvillar apical membrane
and lateral desmosome adhesions. A marked accumulation of mitochondria was also observed. Fluorometric
analysis using the mitochondrial probes nonyl-acridine
orange and JC-1 confirmed a progressive increase in
mitochondrial mass. However these cells also demonstrated a progressive decline in unit mitochondrial transmembrane potential (m) as determined by the
m-sensitive fluorescent probes rhodamine 123 and
JC-1 analyzed for red fluorescence. In concert with
these mitochondrial changes, Colo-205 cells treated with herbimycin A produced increased levels of reactive oxygen species as evidenced by oxidation of both
dichlorodihydrofluorescein diacetate and dihydroethidium. Cell-free assays for apoptosis using rat-liver nuclei
and extracts of Colo-205 cells at 24 h showed that apoptotic activity of Colo-205 lysates requires the early action of mitochondria. Morphological and functional mitochondrial changes were observed at early time points,
preceding cleavage of poly (ADP-ribose) polymerase.
These results suggest that apoptosis in differentiated Colo-205 cells involves unrestrained mitochondrial proliferation and progressive membrane dysfunction, a novel mechanism in apoptosis.
CELLS in hematopoietic and epithelial lineages maintain tissue homeostasis by a dynamic equilibrium
balancing cell proliferation and cell death (91).
Cell death in these lineages has been recognized as apoptotic and generally occurs after a terminal differentiation event (17, 126). These physiological cell deaths are remarkably consistent in both timing and location. Mature
circulating neutrophils acquire apoptotic morphologies by
30 h after release from the bone marrow (28). Enterocytes
undergo apoptosis at villus tips in the small intestine and
at the colonic surface after terminal differentiation and
transit along the crypt-villus axis (32, 43, 90).
The control of apoptosis after terminal differentiation in
these systems is essentially unknown. Although down regulation of expression of bcl-2 and its homologues appears
to trigger other instances of physiological apoptosis (43,
66), the patterns of bcl-2 expression in these lineages with
rapid cell turnover do not explain the observed patterns of
cell death (93). Myeloid expression of bcl-2 is down regulated in metamyelocytes and immature neutrophils (23).
Expression of bcl-2 within small and large intestinal epithelia is confined to the lower 1/3 of the crypts (43). Thus,
down regulation of bcl-2 significantly precedes apoptotic
cell turnover as well as the completion of terminal differentiation. The topographic distributions of the other bcl-2 family members reported to date also do not provide an
explanation for the coordinated apoptosis of terminally
differentiated cells (8, 55).
Cancer cells often have an immature phenotype representing a block in the normal differentiation pathway (6).
Treatments capable of inducing differentiation have been
discovered for cancer cell lines in vitro and have in some
cases been developed as anticancer therapies (6, 16, 112).
In the most successful application of this strategy, all-trans
retinoic acid induces differentiation of acute promyelocytic leukemias carrying the t(1;19) translocation (22, 26).
Complete remissions have been observed with this therapy. Cells that have differentiated in this manner only remain viable for a few days, eventually undergoing apoptosis. Restoration of a normal differentiation program in
cancer cells thus appears to also activate an apoptotic
mechanism similar to the normal physiological process (83).
Herbimycin A, an antibiotic of actinomycetes origin belonging to the ansamycin group (84), is a natural tyrosine
kinase inhibitor that has been reported to induce differentiation in several cell lines, including Rous sarcoma virus-transformed rat kidney cells (76, 118, 119, 122), Rous sarcoma virus-transformed chicken embryo fibroblasts (120),
and human K562 myelogenous leukemia cells (45). Recently, we have observed herbimycin A to induce differentiation of Colo-205, a human colon adenocarcinoma cell
line derived from malignant ascites, in association with inhibition of constitutive src and lck tyrosine kinase activities (Paty, P., and N. Rosen, unpublished observations). In
the same model, cell death by apoptosis follows differentiation (Mancini, M., and B. Anderson, unpublished observation).
In the present study, we describe striking increases in
mitochondrial number within herbimycin-treated Colo-205 cells. Unexpectedly, increased mitochondrial mass did
not lead to cellular accumulation of fluorescent probes
that reflect mitochondrial transmembrane potential ( Tissue Culture
The human colon adenocarcinoma cell line Colo-205 was used for all the
experiments (108). The cells were maintained in RPMI supplemented
with 10% fetal bovine serum (GIBCO BRL, Gaithersburg, MD), 100 U/ml
of penicillin, 100 ng/ml streptomycin, and 2 mM L-glutamine, and grown in
75-cm2 tissue culture flasks at 37°C in a humidified 5% CO2 atmosphere. They were refed every 48 h and passaged weekly.
Chemicals
Herbimycin A (GIBCO BRL) was dissolved in DMSO at a concentration
of 1 mg/ml, stored in 50-µl aliquots at Drug Treatment of Colo-205 Cells
Experiments were carried out by subculturing Colo-205 cells at a density
of 1 × 105 cells/ml in 10 ml medium/100-mm tissue culture plate. The cells were grown for 24 h before drug treatment. Cells were treated with herbimycin A (300 ng/ml) or vehicle (1% DMSO) and were refed with media
containing drug or vehicle at 48-h intervals. Cells were harvested at 0, 24, 48, or 96 h after treatment, collecting both adherent and nonadherent
cells. Adherent cells were collected either using 0.02% trypsin with 5 mM
EDTA or by gentle scraping with a rubber cell lifter. For the NAC experiments, cells were treated with herbimycin or DMSO, and NAC was added
to the cells at time zero at a final concentration of 500 µM.
Transmission Electron Microscopy
Cells were harvested in PBS by gentle scraping and were pelleted by centrifugation. The cell pellets were resuspended in half-strength Karnovsky's fixative for 4-6 h, rinsed in 0.1 M cacodylate buffer, and post-fixed in 1% collidine-buffered osmium tetroxide. Dehydration in graded
ethanol and propylene oxide was followed by infiltration and embedding
in Epon 812. Approximately 70-90-nm sections were stained using saturated, aqueous uranyl acetate and lead tartrate. Photographs were taken
using a transmission electron microscope (100 SX; Jeol, Ltd., Tokyo, Japan) operating at 80 kV.
Detection of Apoptosis
In Situ Labeling of Free 3 Diphenylamine (DPA) Assay for DNA Fragmentation (10).
Cells were
detached from the plates by scraping and washed in PBS. 5 × 106 cells
were resuspended in 500 µl of lysis buffer (5 mM Tris, pH 8, 20 mM
EDTA, 0.5% Triton X-100) for 15 min on ice. Lysed cells were then centrifuged at 12,000 rpm in a microcentrifuge for 30 min at 4°C, and the supernatant and pellet were separately analyzed by the DPA assay as previously described (42).
Western Blot Detection of Poly (ADP-ribose) Polymerase (PARP) Cleavage.
Cells were harvested by trypsinization and centrifuged at 1,000 rpm
for 10 min. 1 × 105 cells were heated for 15 min at 65°C in reducing loading buffer (62.5 mM Tris-HCl, pH 6.8, 6 M urea, 10% glycerol, 2% SDS,
0.003% bromophenol blue, 6% freshly added 2-mercaptoethanol) and
loaded on a 6% SDS-polyacrylamide gel. Proteins were then electro-transferred onto a nitrocellulose membrane and analyzed by immunoblotting. The membrane was incubated with Blotto (5% nonfat dry milk, 0.1% Tween 20 in PBS) for 1 h and then overnight with 1:10,000 dilution in
blocking buffer of anti-PARP monoclonal antibody (C2-10; Enzyme BioSystems, Beloit, WI), followed by 30-min incubation with HRP-conjugated secondary anti-murine IgG (Jackson ImmunoResearch Laboratories, West Grove, PA), diluted 1:2,500 in blotto. The signal was detected using enhanced chemiluminescence (ECL; Amersham Intl., Buckinghamshire, England).
Cell Cycle Analysis
Cell cycle analysis was performed by univariate flow cytometry using either nuclei or whole cell preparations. For nuclei, asynchronously growing
cells were harvested without trypsinization in PBS supplemented with 1%
FCS, washed, and resuspended at a concentration of 1 × 106 cells in 1.5 ml
of ice-cold PBS. After centrifugation, supernatants were discarded, and
the cell pellets were resuspended in 250 µl of Buffer A (10 mM Tris-HCl,
pH 7.5, 20 mM NaCl, 20 mM MgCl2) and incubated on ice for 5 min. 250 µl of Buffer B (Buffer A plus 1% NP-40) were then added to the cell suspension for 5 min on ice. After adding 10 µg/ml of DNase-free RNase, nuclei were incubated at 37°C for 30 min. DNA staining was obtained with
500 µl of propidium iodide solution (PI; Molecular Probes, Eugene, OR)
in PBS (100 µg/ml PI, 0.1% Triton X-100, 1% FCS) for 1 h at 4°C in the
dark, followed by flow analysis. Whole viable cells were also used for cell
cycle analysis and stained with Hoechst 33342 (Sigma Chemical Co.).
Cells were incubated in culture with 0.5 µg/ml of Hoechst 33342 for 90 min at 37°C and then harvested by trypsinization and used for flow analysis. To obtain better preservation of cytosolic DNA in apoptotic cells,
whole cells were analyzed after fixation with 1% paraformaldehyde (pH 7.4)
for 15 min on ice, followed by 70% ethanol. After being washed twice with
PBS, the cells were stained with PI according to the protocol described below, and analyzed by flow cytometry. The DNA fluorescence was measured using a FACScan® (Becton Dickinson, Bedford, MA) for PI or
FACS® Vantage for Hoechst staining. Data acquisition was performed using the Cell Quest software (Becton Dickinson) and data analysis using
the Phoenix Flow System Multicycle AV software.
Assessing Relationship between Apoptosis and Cell Cycle.
At each time
point, the cells were harvested by trypsinization, washed in PBS, and fixed
in 1% formaldehyde (pH 7.4) for 15 min on ice. At the end of the formaldehyde fixation the cells were washed twice in PBS, forcibly pipetted into
5 ml of 70% ethanol, and stored at Assessment of Mitochondrial Changes
Relative mitochondrial mass was measured by flow cytometry using 10-n-nonyl-acridine orange (NAO; Molecular Probes; 67, 88) or 5,5 NAO Staining.
Cells were trypsinized, resuspended in 0.5 ml of PBS,
fixed in 70% ethanol, and stored until use at Rhodamine 123 Staining.
Cells were trypsinized, resuspended in medium at a concentration of 1 × 106/ml, incubated for 30 min at 37°C with
0.5 µg/ml of rhodamine 123, and submitted immediately for flow analysis.
JC-1 Staining.
1 × 106 cells were resuspended after trypsinization in 1 ml of medium and incubated with 10 µg/ml of JC-1 for 10 min at 37°C before flow analysis. Both red and green fluorescence emissions were analyzed after JC-1 staining.
H2-DCF-DA and DHE Staining.
Cells were incubated with 5 µM H2-
DCF-DA or DHE in tissue culture medium for 45 min at 37°C and then
washed, resuspended as 1 × 106/ml in PBS, and submitted to flow analysis.
Flow Cytometric Analysis.
A FACScan® (Becton Dickinson, Bedford,
MA) was used for all the experiments. Forward and side scatters were
used to establish size gates and exclude cellular debris and apoptotic cells
from the analysis (13, 20). For each experiment both herbimycin- and
DMSO-treated cells were analyzed. The excitation wavelength was 488 nm. The observation wavelengths were 530 nm for green fluorescence and
585 nm for red fluorescence. Relative change in mean fluorescence was
calculated as the ratio of mean fluorescence channels for herbimycin to
DMSO-treated samples.
Cell-free Assay for Apoptosis
Cells were harvested by trypsinization at 0, 24, and 48 h after herbimycin
exposure. 5 × 106 cells were rinsed in PBS, and the pellets were resuspended in 300 µl of lysis buffer (20 mM Hepes-KOH, pH 7.5, 10 mM KCl,
1.5 mM MgCl2, 1 mM sodium EDTA, 1 mM sodium EGTA, 1 mM DTT,
0.1 mM PMSF, supplemented with 2 µg/ml aprotinin, 5 µg/ml pepstatin A, 10 µg/ml leupeptin). The samples were left on ice for 15 min and then
lysed by 23 strokes with the B pestle in a Dounce homogenizer (Kontes
Glass Co., Vineland, NJ), and centrifuged at 1,000 g for 10 min at 4°C. The
resulting pellet (nuclear fraction) was discarded, and the supernatant (cytosolic fraction) was split in two parts and used for the in vitro apoptosis assay.
Protein content of the cell extract was determined by the Lowry method.
The lysates were adjusted to a fixed protein concentration (5 µg/µl). Half of
each lysate was further centrifuged at 10,000 g for 30 min at 4°C. The resulting supernatant (S10 fraction) was collected. A preparation of rat liver nuclei
containing 200,000 nuclei/µl of buffer A (10 mM Pipes, pH 7.4, 80 mM KCl,
20 mM NaCl, 5 mM sodium EGTA, 250 mM sucrose, 1 mM DTT) was
kindly provided by Michael Wright (University of Washington, Seattle,
WA). The cell-free reactions were set up in Eppendorf tubes containing 1 µl
of nuclear preparation (200,000 nuclei) and 30 µl of Colo-205 lysate (whole cytosolic fraction or S10 fraction), corresponding to 150 µg of protein. The
total volume was adjusted in each tube to 50 µl using dilution buffer (10 mM Hepes, 50 mM NaCl, 2 mM MgCl2, 5 mM EGTA, 1 mM DTT). The
samples were incubated for up to 2 h at 37°C and then an aliquot of each reaction (10 µl) was mixed 1:1 with staining solution (200 mM sucrose, 5 mM
MgCl2, 80 mM KCl, 15 mM NaCl, 5 mM EDTA, 150 mM Pipes-NaOH, pH
7.4, 10 µg/ml Hoechst 33258, 3.7% paraformaldehyde) and observed for nuclear morphological changes by fluorescence microscopy.
Statistics
Data are reported as mean ±SD except where indicated. Experiments
were performed in triplicate. Comparisons among multiple groups were
made by a one-way analysis of variance (ANOVA) followed by Fisher's
protected least significant difference post-hoc test. 95% confidence limits
(P Herbimycin A Induces Growth Arrest Followed by Cell
Death in Colo-205 Cells
Colo-205 cells treated with herbimycin A in a dose range
of 200-500 ng/ml demonstrate growth arrest, evident by 24 h
after treatment. A dose of 300 ng/ml produces maximal
growth arrest and differentiation, along with maximal inhibition of src and lck tyrosine kinase activities in Colo-205
cells (Paty, P., and N. Rosen, unpublished observations).
Cell count, representing the net result of cell proliferation
and cell death, is decreased at 24 h in herbimycin-treated
cells compared to cells treated with DMSO alone (Fig. 1
a). Loss of cell viability, as measured by trypan blue exclusion, is not apparent in herbimycin-treated cells until 48 h
(Fig. 1 b), suggesting that the early decrease in cell count
in herbimycin-treated cells results primarily from decreased proliferation rather than accelerated death. This
finding is confirmed by cell cycle analysis which shows that
herbimycin causes fewer cells to be in S phase at 24 h in
comparison to control, accompanied by accumulation of
the cells in G2/M at 24-48 h (see Fig. 6 and text below).
Both cell count and cell viability continue to decrease
throughout the 96-h observation period (Fig. 1, a and b).
Herbimycin A-induced Epithelial Differentiation Is
Associated with Mitochondrial Proliferation
Colo-205 cells appear as small, round cells with scattered
spindle-shaped cells by light microscopy. These cells grow
as semi-adherent cells in plastic tissue culture flasks. Addition of herbimycin A triggers a sequence of major morphological changes in Colo-205 cells. At 24 h the cells are
adherent and appear larger, flattened, and less rounded.
They form aggregates and epithelioid colonies, which become progressively larger from 24 to 96 h (Fig. 2).
Transmission electron microscopy demonstrates the ultrastructural characteristics of Colo-205 cells. Before herbimycin exposure, they appear as isolated, small, rounded
cells, with a high nucleus-to-cytoplasm ratio and a simple
cytoplasmic organization with a paucity of organelles (Fig.
3 a). By 24 h after herbimycin treatment, Colo-205 cells
begin to acquire ultrastructural features of differentiation (Fig. 3 b). They appear larger and form a rudimentary polarized monolayer with an emerging brush border. There
is a general increase in free ribosomes and rough endoplasmic reticulum. Some cells at 24 h were noted to have
increased numbers of mitochondria, many of which are
electron dense and have reduced volume as compared to
control cells (Fig. 3 b).
The differentiated phenotype of Colo-205 cells has progressed by 48 h of herbimycin treatment, when the cells assume a more mature enterocytic morphology that resembles that of the columnar absorptive lineage (77). In
particular, the cells acquire a polygonal shape, and cell polarity and the apical brush border are better defined, with
well-formed desmosomes connecting adjacent cells close
to their apical surface (Fig. 3 c). The number and condensation of mitochondria appear further increased at this time point.
At 96 h, surviving cells are found at the free surface of
larger cellular aggregates and appear highly differentiated
with long, narrow, and more numerous apical microvilli,
while underlying cells are apoptotic or appear to be undergoing secondary necrosis (see below). The superficial cells
of these clusters have condensed cytoplasm and decreased
intercellular contacts as compared to cells after 48 h of
treatment (Fig. 3 d). Zones of detachment between adjacent cells resemble those observed in terminally differentiated enterocytes of normal colonic mucosa (90). Surprisingly, after 96 h of herbimycin exposure, viable cells show
still higher numbers of highly condensed small mitochondria, localized primarily to the apical side of cells (Fig. 3 d).
Apoptosis Follows Herbimycin-induced
Epithelial Differentiation
Between 48 and 96 h after herbimycin exposure, light microscopy showed a progressive reduction in cellular density, and both intact dead cells and cellular debris were visible (Fig. 2).
As the differentiation process continues, apoptotic figures become increasingly visible by electron microscopy.
While barely detectable at 24 h (8/400 cells), intact apoptotic cells (Fig. 4 a) and apoptotic bodies (Fig. 4 b) are
readily apparent from 48 to 96 h. Apoptotic cells demonstrate intact cell membranes with cytoplasmic and chromatin condensation (Fig. 4 a). Apoptotic bodies contained either highly condensed chromatin typical of apoptosis or
finely dispersed chromatin, resembling a chromatolytic morphology. These latter changes were consistent with
post-apoptotic or secondary necrosis (68).
The presence of delayed apoptosis was confirmed by
other assays. Tunel, a specific, histologic method for detection of nuclear DNA fragmentation (32), identified 15%
of nuclei as apoptotic 48 h after herbimycin addition to the
cells (Fig. 4 c). The DPA assay, a quantitative measure of
soluble DNA released from apoptotic nuclei into the cytoplasm (10), demonstrated DNA fragmentation at 48 and
96 h (Table I). Similarly, DNA histograms of PI-stained Colo-205 nuclei showed a progressive increase in events
with sub-diploid DNA content (the "sub-G1 peak") which
is characteristic of apoptosis (82). The frequency of sub-G1
events was 0, 3, 15, and 42% at 0, 24, 48, and 96 h, respectively (Fig. 5).
Table I.
Percentage Cytosolic DNA (Dpa Assay)
These results permit us to identify a sequence of herbimycin-induced events in Colo-205 cells. Growth arrest and
differentiation are observed by 24 h. At 48 h, cellular viability begins to decrease, and apoptosis, quantified by
Tunel, DPA, and frequency of sub-G1 events, is detectable. At 96 h the general viability is further reduced and
apoptosis increased. Thus, growth arrest, differentiation, and apoptosis proceed sequentially in this model. Mitochondrial number increases progressively at each stage,
starting at 24 h. During the program of terminal differentiation of normal colonic mucosa, cell cycle arrest in G1 precedes differentiation and apoptosis (27). To determine the
effect of herbimycin on cell cycle progression, cell cycle
analysis of Colo-205 cells was performed.
Cell Cycle Events: G2/M Accumulation and Its
Relationship to Herbimycin-induced Apoptosis
Cell cycle distribution in Colo-205 cells after exposure to
herbimycin was assessed by flow cytometric measurement
of cellular DNA content. Analysis was carried out first on
isolated nuclei stained with PI. In this case cell cycle analysis showed reduction of S phase at 24 h and subsequently,
appearance of a progressively larger sub-G1 population
(sub-G1 peak), indicative of apoptosis (Fig. 6, top row).
A different result was obtained when flow analysis was
performed on whole cells, either viable and stained with
Hoechst 33342 or fixed (1% paraformaldehyde and 70%
ethanol) and stained with PI (18). A better preservation of
the degraded, low molecular weight DNA inside the apoptotic cells can be achieved in this way (20, 21). Cell cycle
analysis of whole cells showed early reduction of S phase
at 24 h, as already seen by analyzing nuclei. At 24-48 h,
however, an accumulation of cells in G2/M is evident (Fig.
6, middle and bottom rows). Careful analysis of the raw data showed a wide shoulder of events at the left of the G2/M peak at 48 h, suggesting a concealed sub-G2 peak (Fig. 6,
middle and bottom rows). Indeed, a population of cells
with a DNA content below the normal G2/M content can
be detected. Because of its shape and also the observed
early decrease in S phase, this peak is most likely due to
DNA fragmentation of the G2 cells and could be defined
as a "sub-G2 peak". A sub-G2 peak is not evident when the
analysis is performed on nuclei (Fig. 6, top row), where a larger sub-G1 peak predominates. This is probably due to
extraction and loss of low molecular weight DNA fragments during the preparation of nuclei from the apoptotic
cells in G2 (1, 20). Consistent with this hypothesis, nuclei
preparations do not show an increased G2/M peak.
Fixation of whole cells with paraformaldehyde allowed
a better preservation of the sub-G2 peak (Fig. 6, bottom
row). Conforming with the previously shown increase in
apoptosis at 96 h, the sub-G2 peak is increased at this time
point. The G2 peak is decreased, also suggesting that apoptosis is depleting G2 cells. The widened G1 peak present in
fixed cells at 96 h (Fig. 6, bottom row) probably represents
sub-G1 and G1 populations similar to the one represented in Fig. 6, middle row. Due to software limitations, it is not possible to discriminate sub-G1 together with sub-G2 populations in this sample.
Simultaneous measurement of DNA content (cell cycle)
and apoptotic strand breaks (DNA fluorescein-dUTP labeling) was also performed (34, 64). While absent at 0 and
24 h, TdT labeling is confined to the population of cells in
G2 and sub-G2 at 48 h (Fig. 7). This result confirms that apoptosis begins in Colo-205 cells residing in G2 after herbimycin treatment. By 96 h, the extensive DNA degradation
may not be detectable in G2 cells by the TdT assay of
DNA strand break labeling because very excessively fragmented, small molecular weight DNA cannot be retained
in the cells and is extracted during the sample preparation
(Darzynkiewicz, Z., personal communication).
Cell cycle analysis, in conclusion, shows an early decrease in S phase, with G2 accumulation at 24-48 h and evidence of apoptosis beginning in G2 at 48 h.
Herbimycin A Induces Increased
Mitochondrial Mass but Decreased Relative
Mitochondrial Transmembrane Potential
The mitochondrial abnormalities observed by electron microscopy during herbimycin-induced differentiation were
further evaluated using specific fluorescent mitochondrial
probes. To measure changes in mitochondrial mass, cells
were stained at different time points with NAO (67), a fluorescent dye that specifically binds to the mitochondrial
inner membrane independent of energetic state ( Table II.
Mean Fluorescence Values of Mitochondrial Mass,
m).
These results are consistent with impaired function of
newly synthesized mitochondria. Morphologic studies and
examination of cell-free assays indicate that progressive,
structural changes to mitochondria occur during proliferation and coincide with apoptotic activity in cell lysates.
Materials and Methods
20°C, protected from light, and
thawed immediately before use. N-acetyl-L-cysteine (NAC; Sigma Chemical Co., St. Louis, MO) was dissolved in doubly distilled water (100 mM,
pH 7.4), stored in aliquots at
20°C, and thawed immediately before use.
-Hydroxyl Ends on DNA.
Cells were harvested by
gentle scraping, washed in PBS, and counted. An aliquot of 100,000 cells
was resuspended in culture media and transferred to a microscope slide by
cytospin (800 rpm for 5 min). Cells were fixed in 4% buffered formaldehyde, and fragmented DNA was labeled using a modification of the
method of terminal deoxynucleotidyl transferase (TdT)1-mediated dUTP-biotin nick end-labeling (TUNEL), described by Gavrieli et al. (32).
Briefly, nuclei of cell preparations were permeabilized by incubation with
80 µg/ml proteinase K for 10 min at room temperature. Endogenous peroxidases were inactivated by covering the sections with 2% H2O2 for 5 min at room temperature. After incubation for 5 min in TdT buffer (30 mM Tris, pH 7.2, 140 mM Na-cacodylate, 4 mM MgCl2, 0.1 mM DTT), the
slides were covered with TdT (0.3 U/µl) and biotinylated dUTP in TdT
buffer and incubated for 60 min at 37°C in a humidified chamber (TdT enzyme and dUTP obtained from Boehringer Mannheim, Indianapolis, IN).
Negative controls were incubated with biotinylated dUTP in TdT buffer
without enzyme. The reaction was terminated by transferring the slides to
TB buffer (300 mM NaCl, 30 mM Na citrate) for 15 min at room temperature. The sections were covered with 2% BSA for 10 min at room temperature and immersed in PBS for 5 min followed by incubation with (1:1,000
dilution in PBS) HRP-streptavidin (Zymed Labs., Inc., S. San Francisco,
CA) for 30 min at 37°C. The sections were then covered with DAB catalyzed with NiCl2 and H2O2 for 10 min and counterstained with 0.25%
methyl green in PBS. Positive controls were incubated for 10 min with
DNase (100 ng/ml, dissolved in DN buffer: 30 mM Tris-HCl, pH 7.2, 140 mM Na-cacodylate, 4 mM MgCl2, 0.1 mM DTT) before exposure to TdT. After counterstaining, the apoptotic cells were quantified by counting a
minimum of 200 cells from at least 5 different random areas of the slide.
20°C until ready for use. On the day
of the experiment, the cells were washed twice in PBS and resuspended in
50 µl of TdT reaction buffer containing 10 µl of 5× concentrated buffer solution (1 M sodium cacodylate, 125 mM Tris-HCl, pH 6.6, 1.16 mg/ml
BSA), 5 µl of 25 mM cobalt chloride, 12.5 U of TdT enzyme, 0.25 nmoles
of fluorescein-dUTP (TdT enzyme and fluorescein-dUTP were both obtained from Boehringer Mannheim), and doubly distilled water up to 50 µl (34, 64). The cells were incubated in this solution for 60 min at 37°C and
then rinsed twice with 1.5 ml of 15 mM EDTA, pH 8, and once with 1 ml
of 0.1% Triton X-100 in PBS. At the end of the washing, DNA counterstaining was carried out by resuspending the cellular pellets in 1 ml of PI
solution in PBS (2.5 µg/ml PI, 10 µg/ml DNase-free RNase) and incubating them for 30 min at room temperature, shielded from light.
,6,6
-tetrachloro-1,1,3,3
- tetraethylbenzimidazolcarbocyanine iodide (JC-1; Molecular Probes; 94, 111), analyzed for green fluorescence. Mitochondrial function was indirectly assessed by variation in mitochondrial transmembrane
potential measured by rhodamine 123 (14, 49) and JC-1 red fluorescence.
ROS production was assessed by oxidation of 2
,7
-dichlorodihydrofluorescein diacetate (H2-DCF-DA; Molecular Probes) and dihydroethidium
(DHE; Molecular Probes) to fluorescent products 2
,7
-dichlorofluorescein (DCF) and ethidium (Eth), as measured by flow cytometry (5, 44, 99,
127).
20°C. On the day of each experiment, ethanol was removed by centrifugation, and 1 × 106 cells
were washed in PBS and stained with 10 µM NAO in 1 ml of PBS. After
incubating for 10 min at room temperature in the dark, cells were washed
in PBS and submitted for flow analysis.
0.05) were considered significant.
Results
Fig. 1.
Growth and viability curves of Colo-205 cells treated with herbimycin A, 300 ng/ml (solid line), or DMSO vehicle, 1% (dotted line). Cell growth, as expressed by number of viable cells/ml media (A) and cell viability, as measured by trypan blue exclusion (B), progressively decrease in cells treated with herbimycin A. Growth arrest starts 24 h before the decrease in viability becomes evident, suggesting that herbimycin decreases cell proliferation and then induces cell death.
[View Larger Versions of these Images (9 + 9K GIF file)]
Fig. 6.
Cell cycle analysis
of Colo-205 cells after 0, 24, 48, and 96 h of incubation
with herbimycin A, as assessed by analysis of DNA
content and represented in
histograms plotting DNA
content versus cell number.
Nuclear preparations stained
with propidium iodide were
analyzed first (top row). The
histograms show an early decrease in S phase and a progressively larger sub-G1 peak,
characteristic of apoptosis.
Analysis of viable whole cells
stained with Hoechst 33342 (middle row) revealed a G2/
M accumulation starting at
24 h of treatment and peaking at 48 h. At this time point,
a sub-G2 peak is detected by
the computer software, suggesting beginning apoptosis
in G2/M. Fixation of whole
cells with 1% paraformaldehyde, followed by propidium
iodide staining, shows a better preservation of the sub-G2 peak at 48 and 96 h (bottom row).
[View Larger Version of this Image (38K GIF file)]
Fig. 2.
Phase contrast light microscopy images of Colo-205 cells during culture with herbimycin A (300 ng/ml). The small, round
Colo-205 cells visible at time 0 (a) show increase in size and tendency to form cellular aggregates at 24 h. At 48 h (c), the cells appear organized in epithelioid colonies, which appear larger at 96 h (d). Dead cells and cellular debris can be observed in culture at 48 and 96 h
of herbimycin exposure.
[View Larger Version of this Image (110K GIF file)]
Fig. 3.
Transmission electron microscopy images of Colo-205 cells illustrating the differentiation process induced by in vitro exposure to herbimycin A (300 ng/ml). Untreated cells appear undifferentiated (a). By 24 h after herbimycin treatment (b), the cells tend to form
a polarized monolayer with an emerging brush border (arrows). Some cells at this time point show increased numbers of small electron-dense mitochondria (arrowheads). By 48 h, cells develop a more differentiated, polarized phenotype (c) with well formed desmosomes
connecting adjacent cells (arrows). Some mitochondria show signs of degeneration and derangement of the membranes (arrowheads). At
96 h (d), adjacent differentiated cells show many highly condensed, small mitochondria and zones of intercellular detachment (asterisks).
[View Larger Versions of these Images (100 + 147K GIF file)]
Fig. 4.
Electron and light microscopic images illustrating herbimycin-treated Colo-205 cells undergoing apoptosis. A whole
apoptotic cell (a) has a mostly intact cellular membrane with cytoplasmic shrinkage and condensation. The nucleus appears pyknotic and fragmented (arrows). The cell also seems to be undergoing fragmentation (arrowheads), forming an apoptotic body.
Apoptotic bodies at various stages of degeneration (b); some
contain highly condensed chromatin (arrowhead), and others appear to be nuclear fragments undergoing chromatolysis (arrow),
as in post-apoptotic necrosis. Tunel staining of Colo-205 cells at 48 h
after herbimycin treatment labels apoptotic nuclei in black (c).
[View Larger Version of this Image (110K GIF file)]
Fig. 5.
The frequency of sub-G1 events (percentage of particles in the "sub-G1 peak") obtained analyzing nuclear preparations from Colo-205 cells after 0, 24, 48, or 96 h of treatment with
herbimycin A (300 ng/ml) or DMSO vehicle (1%). The increasing number of events with sub-G1 DNA content in herbimycin-treated samples is characteristic of apoptosis.
[View Larger Version of this Image (23K GIF file)]
Fig. 7.
TdT flow cytometry of Colo-205 cells after 0, 24, 48, and 96 h of incubation with herbimycin A. The graphs plot DNA content on the x-axis and TdT-labeled apoptotic DNA strand breaks on the y-axis. No increase in TdT labeling above baseline is apparent at 24 h
after addition of herbimycin. By 48 h, a population of cells with increased TdT-labeling is present, corresponding to the cells with DNA
content in the sub-G2 and G2/M phase of the cell cycle. By 96 h, extensive DNA loss due to massive cell death is represented as a shift to
the left on the DNA histogram, preventing significant TdT labeling at this time point.
[View Larger Version of this Image (14K GIF file)]
m).
Herbimycin-treated cells showed a progressive increase in
mean NAO fluorescence with time (Table II), consistent
with the increased number of mitochondria per cell observed on electron microscopy. In contrast, rhodamine 123 fluorescence, an indicator of
m (14, 49), did not increase
after herbimycin treatment. Thus, the cellular mitochondrial transmembrane potential per unit mitochondrial
mass, expressed as the ratio of rhodamine 123 to NAO fluorescence, decreased throughout the 96-h experimental
period (Table II).
m , and ROS Production in Herbimycin-treated Cells
(Relative to DMSO Control Cells)
To control for potential differences in NAO/rhodamine
123 loading, we repeated the mitochondrial staining experiments using the J-aggregate forming lipophilic cation JC-1
(Fig. 8, a and b). This mitochondrial dye, which normally
exists in solution as a monomer emitting a green fluorescence, assumes a dimeric configuration emitting red fluorescence in a reaction driven by mitochondrial transmembrane potential (94, 111). Thus, the use of JC-1 allows
simultaneous analysis of mitochondrial mass (green fluorescence) and mitochondrial transmembrane potential (red fluorescence). After treatment with herbimycin we
again observed a progressive dissociation between mitochondrial mass and m, with incremental increases in JC-1
green fluorescence without a corresponding increase in
JC-1 red fluorescence. Like the rhodamine 123 to NAO ratio,
the JC-1 red to green fluorescence ratio decreased with
time as differentiation and apoptosis progressed (Table II).
The consistent results of the mitochondrial staining experiments, while confirming the increase in mitochondrial mass observed by electron microscopy, showed a decrease in unit mitochondrial transmembrane potential. This pattern has been associated with mitochondrial uncoupling and increased mitochondrial production of ROS (127). We subsequently tested whether ROS production increased in this model.
Herbimycin A Induces Increased ROS Generation
We tested the production of ROS by staining with DHE
and H2-DCF-DA, which are oxidized in the presence of
superoxide (O2) and peroxides, respectively, to fluorescent products (5, 44, 99, 127). Production of O2
and peroxides in herbimycin-treated Colo-205 cells increased with
time (Fig. 9, and Table II). At 24 h, before detection of apoptosis, an increase in ROS production was already apparent, corresponding to the first decrease in the ratio of JC-1
red and green fluorescence. This correlation was maintained at later times, as the population continued to show
an increase in ROS production and a proportional decrease in JC-1 red to green ratio (Fig. 9, and Table II).
Sequence of Ultrastructural Changes in Mitochondria during Herbimycin Exposure
Sequential changes in mitochondrial ultrastructure were
identified in differentiating Colo-205 cells by electron microscopy. On the electron micrographs, the extent of nuclear chromatin condensation can be correlated with
changes in mitochondrial number and structure within the
same cell. Before addition of herbimycin, the mitochondria appear oval shaped, with a finely granular matrix containing a few dense granules (Fig. 10 a). The cristae are
sparse, and the mitochondria are occasionally adjacent to
endoplasmic reticulum cisternae.
24 h after addition of herbimycin, the increase in mitochondrial number is associated with more elongated forms. The matrix is condensed, and the cristae are well formed, often traversing the mitochondrion. Wide cisternae of rough endoplasmic reticulum are wrapped around mitochondria, and a general increase in the number of polysomes in the cytoplasm is observed (Fig. 10 b). This feature suggests an increase in protein synthesis and mitochondrial import during the early stages of herbimycin-induced apoptosis. Lysosome-like vacuoles containing myelin figures are sometimes seen juxtaposed to mitochondria/rough endoplasmic reticulum complexes and may represent ongoing autophagocytosis of damaged organelles (33). Nuclear chromatin in the cells appears normal at this stage.
As differentiation proceeds through 48 h, the mitochondria become more numerous, small and condensed, while the associations with rough endoplasmic reticulum cisternae progressively decrease (not shown). Deranged cristae suggest the development of mitochondrial degeneration (Fig. 3 c). Overall these changes are suggestive of extensive mitochondrial proliferation, coupled with matrix condensation and volume loss.
Mitochondrial ultrastructure in differentiated cells at 96 h is characterized by hypercondensation of the matrix and further reduction in size, with high numbers of mitochondria packed in the cytoplasm (Fig. 11 a). The outer mitochondrial membrane appears indistinct, and internal structures are obscured by the highly electron-dense matrix. The close association between mitochondria and rough endoplasmic reticulum is no longer seen. The nuclear chromatin at this stage is condensed and marginated.
Finally, a spectrum of degenerative changes is noted in the mitochondria of cells in advanced apoptosis 96 h after treatment with herbimycin. This pattern is characterized by mitochondrial swelling and loss of matrix density. The nuclear chromatin and the cytoplasm are also less condensed, and many cells have a morphological appearance characteristic of post-apoptotic necrosis (Fig. 11 b). As the nuclear chromatin appear less condensed, the mitochondria show enlargement of the cristae (intracristal swelling or ballooning). The matrix appears still condensed, but to a lesser degree than before, while the total mitochondrial volume does not appear increased (Fig. 11 c). Chromatolysis is associated with a more advanced mitochondrial pathology (Fig. 11 b). Widening of the intracristal space becomes more evident, and the mitochondria show a high degree of swelling and appear electron lucent and more rounded (Fig. 11 d). The matrix contains flocculent densities, and the outer membrane is often discontinuous. The internal mitochondrial structure becomes visible again and shows some cristae dividing the organelle into two symmetric halves (partition), a feature suggestive of mitochondrial division (33).
NAC Prevents Herbimycin-induced Mitochondrial Changes and Protects the Cells from Apoptosis
We subsequently tested the effect of NAC on Colo-205 cells exposed to herbimycin. NAC has been previously shown to protect cells against damage associated with oxidative stress (3, 73, 74, 113). Colo-205 cells treated with a combination of 500 µM NAC and herbimycin A did not undergo apoptosis (Table I). Herbimycin-induced epithelial differentiation was not affected by NAC, as judged by light and electron microscopic inspection. However, mitochondrial proliferation in response to herbimycin was abolished by co-treatment with NAC. JC-1 green fluorescence and the ratio of red to green fluorescence intensities remained at baseline values under these conditions (Table III). The absence of mitochondrial changes was confirmed by EM, demonstrating a lack of change in mitochondrial number and morphology, despite the development of a characteristic differentiated cellular phenotype (not shown).
Table III.
Mean Fluorescence Values of Mitochondrial Mass
and |
Mitochondria-enriched Fractions Are Required for Apoptotic Activity of Colo-205 Lysates
As a further indicator of the relationship of mitochondrial
changes to the apoptotic program, we tested whether apoptotic "activity" in cell-free assays showed an early dependence on mitochondria from Colo-205 cells. Using rat liver
nuclei to score apoptotic responses, cytosolic extracts were
prepared from Colo-205 cells exposed to herbimycin for 0, 24, and 48 h. Extracts were incubated with the nuclei for 1 h
at 37°C. Extracts of Colo-205 cells at time 0 did not show
apoptotic activity (Fig. 12 a). In contrast, cytosolic extracts
of cells treated with the drug for 24 h induced classical
changes of apoptosis in the target nuclei after 1-h incubation at 37°C (Fig. 12 b). We removed a heavy membrane
fraction containing mitochondria from cell lysates, by centrifugation at 10,000 g for 30 min. The S10 supernatant at
24 h had no demonstrable apoptotic activity in the cell-free assay even after 2 h of incubation with nuclei (Fig. 12
c). 48-h extracts showed similar apoptotic activity to the
24-h samples, but the corresponding S10 supernatant was
still able to induce partial apoptotic responses in the target nuclei (not shown). These results indicate that at 24 h, before irreversible activation of cytoplasmic components, the
apoptotic program induced by herbimycin requires the
early action of mitochondria.
Herbimycin-induced Mitochondrial Changes Precede PARP Cleavage
To assess the activation of ICE family members in Colo-205 cells, we examined cleavage of PARP, a proteolytic
target of the CPP32/Yama protease (81, 115). Cleavage of
PARP to an 85-kD fragment has been described as an
early event in drug-induced apoptosis in mammalian cells
(51). The 85-kD proteolytic fragment of PARP was first
detected in immunoblots of Colo-205 lysates after 48 h of
exposure to herbimycin A (Fig. 13). Changes in mitochondrial morphology, as well as an increase in mitochondrial
numbers and a decrease in the JC-1 red/green ratio have
already occurred by 24 h (Figs. 8 and 10 b, and Table II),
preceding PARP cleavage.
The nonspecific tyrosine kinase inhibitor herbimycin A can induce differentiation in several experimental models (45, 120). Experimentally transformed cell lines treated with herbimycin can also transiently revert to an untransformed phenotype (76, 118, 120, 121). We have been studying herbimycin-induced epithelial differentiation in the human colon cancer cell line Colo-205, associated with inhibition of src and lck tyrosine kinase activities (Paty, P., and N. Rosen, unpublished observations).
In the present study we report that, in addition to differentiation, herbimycin induces apoptotic cell death in Colo-205 cells. Moreover, the two events occur sequentially. Differentiation starts 24 h after herbimycin exposure and appears fully developed at 48 h, while apoptosis becomes detectable at 48 h and peaks at 96 h. This relationship between differentiation and apoptosis in Colo-205 cells appears to mirror the changes occurring in normal colonic mucosa during the physiologic program of terminal differentiation of enterocytes (15, 77). The undifferentiated stem cells localized at the base of colonic crypts give rise to daughter cells that differentiate and migrate up the crypt to reach the mucosal surface, where they die by apoptosis and are sloughed from the colonic surface (32, 90). The physiologic life span of the differentiating cells lasts about 3-5 d (15). Terminal differentiation in the immortalized undifferentiated cell line Colo-205 after in vitro exposure to herbimycin is also followed by apoptosis after a similar time lag, suggesting that the enterocyte pathway of terminal differentiation and apoptosis are tightly linked. In our model, we also observe that a small number of cells undergo apoptosis as early as 24 h. This is consistent with findings in normal intestine where scattered apoptotic cells are detected at multiple levels of the crypt-villus axis, including occasional cells adjacent to the crypt bases (90, 107).
Herbimycin A also induces an accumulation of Colo-205 cells in the G2/M phase of the cell cycle. Apoptosis seems to occur preferentially in G2/M, as demonstrated by the presence of a sub-G2 apoptotic peak and of positive TdT labeling of G2/M cells (20, 34). Another tyrosine kinase inhibitor, genistein, has been shown to induce G2 arrest in vitro (72). We have also observed that genistein induces in Colo-205 cells G2 arrest, differentiation, and apoptosis, similar to herbimycin (Mancini, M., and B. Anderson, unpublished observations). Roche et al. showed that signal transduction pathways involving tyrosine kinases function in mitosis, in particular, src family kinases are required for the G2-M transition (98). As a consequence, one of the direct effects of herbimycin could be to arrest the cells in the G2 phase of the cell cycle.
Terminal differentiation normally occurs when the cells exit the cell cycle and arrest in G1 (27). However, a G1 arrest may not be necessary for commitment to terminal differentiation. A number of drugs known to cause a cell cycle delay in G2 are able to induce terminal differentiation in murine Friend erythroleukemia (FEL) and K562 leukemia cells (24, 130).
Besides typical apoptotic cells and apoptotic bodies, we also observed in our EM preparations, particularly at 96 h, some cells with a phenotype characteristic of cellular necrosis, i.e., chromatolysis, cytoplasmic vacuolization, and disruption of the cellular membrane. We interpret this cellular morphology as a secondary event to apoptosis, or "post-apoptotic necrosis," as recently proposed by Majno and Joris (68). The appearance of a necrotic morphology after apoptosis has been described in embryonic tissues and in dermatopathology (103, 125). Even in the classical model of apoptosis induction described by Kerr, the ligation of the main portal venous trunk to the left and median lobes of the rat liver with consequent liver atrophy and formation of Councilman bodies, these are found to sometimes undergo severe degenerative changes resembling necrosis (52, 53). A possible apoptosis-necrosis sequence has also been recently reported in dystrophin-deficient muscle cells, previously believed to die primarily by necrosis (116). It is not known whether post-apoptotic necrosis occurs during the late stages of terminal differentiation of normal enterocytes. Phagocytosed apoptotic bodies in various stages of lysosomal digestion have been observed in the stratified colonic mucosa of rat embryos (38), but in a study by Potten and Allen on adult mouse small intestinal mucosa, phagocytosis and cellular fragmentation were seen only in cells dying at lower positions along the crypt- villus axis, with a less differentiated phenotype (90). Terminally differentiated cells were shown to be shed at the villus tip after loss of contact with the neighboring cells, without undergoing phagocytosis. Early apoptotic changes, such as nuclear pyknosis and chromatin margination were observed in terminally differentiated enterocytes, but they were mostly extruded as whole cells (90). It is possible that later, once in the bowel lumen, they undergo further degeneration in the form of post-apoptotic necrosis.
Our results show that herbimycin-induced differentiation in Colo-205 cells is associated with an increase in mitochondrial number but without a concomitant increase in
net mitochondrial transmembrane potential. These results
suggest an accumulation of mitochondria with low transmembrane potential. There is scant information available
on the role of mitochondria during terminal differentiation programs. Studies by Heerdt and colleagues have reported increased mitochondrial gene expression in colon
carcinoma cells after induction of differentiation along the
absorptive lineage (39) and that increased mitochondrial
gene expression is a consistent and early event during apoptosis of colonic carcinoma cells in vitro (40). The importance of mitochondrial abnormalities during apoptosis has
been gaining support recently (41), despite the classical
descriptions of apoptosis with morphological preservation of mitochondria (53). The availability of fluorescent dyes
which specifically reflect mitochondrial mass and function
has enabled more detailed studies of mitochondrial modifications during apoptosis (87). Vayssiere et al. have
shown in rat embryo fibroblasts transfected with a temperature-sensitive mutant of SV40 T antigen that reduction of
m is an early event during apoptosis triggered by inactivation of SV40 T antigen (123). Petit et al. have reported similar results in dexamethasone-induced apoptosis of murine thymocytes (89). Apoptosis in peripheral lymphocytes
from mice treated with dexamethasone is correlated with
an early reduction in
m, while classical nuclear features
of apoptosis are not detectable in vivo (127).
The increase in mitochondrial number during herbimycin-induced differentiation in Colo-205 cells is associated with condensation of the mitochondrial matrix and a reduction in total mitochondrial volume. This condensed appearance is notable at early time points in Colo-205 cells, before apoptotic changes in the nucleus. The degree of mitochondrial condensation subsequently increases in concert with the appearance of chromatin and cytoplasmic condensation. At later time points, signs of mitochondrial degeneration are apparent, characterized initially by dilatation of the intracristal space and then by progressive swelling of the mitochondria, with interruption of the outer membrane and loss of matrix density. This sequence of mitochondrial modifications in Colo-205 cells after herbimycin treatment, represented in Figs. 10 and 11, shows a transition from an orthodox, to condensed, to transitional, and finally to swollen conformation (33). Transitional and swollen morphologies are associated with progressive chromatolysis and necrotic degeneration of the cells.
Morphological studies by Hackenbrock and colleagues have shown that a reversible transition of mitochondrial morphology from an orthodox to a condensed conformation is associated with high and low energy states of mitochondria, respectively (35). The condensed morphology is characterized by a reduction in the inner mitochondrial compartment due to increased folding of the inner membrane, with a consequent increase of the outer compartment, intracristal space and without significant reduction of the total mitochondrial volume. Further studies on mitochondrial morphology in response to inhibition of ATP synthesis or cellular injury published by Laiho and colleagues demonstrated a sequence of mitochondrial modifications from the orthodox, to condensed, and eventually to swollen mitochondrial conformations (60, 61). A transitional morphology characterized by increased intracristal space can also be observed between the condensed and the swollen configuration. In these studies the condensed conformation, due to loss of water and ions from the mitochondrial inner compartment, was associated with decreased ATP synthesis, with or without decreased respiration, while the swollen conformation represents a more advanced stage of damage to the inner mitochondrial membrane and is usually associated with cellular necrosis. The condensed conformation was reversible before the "point of no return" is reached. Nevertheless the condensed conformation is unstable, and, in situations of continued injury, the mitochondrial membrane becomes permeable and the organelle swells progressively, with rupture of the outer membrane and destruction of the different components (60, 61). The appearance of a mitochondrial condensed morphology, followed by progressive mitochondrial swelling, has also been recently reported by Papadimitriou et al. to occur during complement-mediated cell death in Ehrlich ascites tumor cells (85).
The sequence of mitochondrial alterations observed in herbimycin-treated Colo-205 cells differs in some respects from that observed by Laiho and Trump (60). At a time when the degree of mitochondria condensation in Colo-205 cells is advanced, the outer and inner mitochondrial membranes appear closely associated, the intracristal space is not enlarged, and the total mitochondrial volume is reduced. This morphology could be described as "mitochondrial pyknosis," characterized by hyperdensity of the matrix and reduction in mitochondrial size (33). It is possible that in our model the pyknotic configuration represents a more extensive and pathological degree of mitochondrial condensation, with contraction of the outer mitochondrial membrane. Mitochondrial pyknosis has been sporadically reported in the literature (30, 69, 100, 101, 114). The term was first employed by Wachstein and Besen in a study of renal tubular coagulative necrosis due to dl-serine intoxication in rats (124). It is interesting to observe that in this paper, published before the first paper on apoptosis by Kerr et al. (53), early focal lesions were identified in which isolated cells showed cytoplasmic condensation, nuclear shrinkage with serrated irregular outlines, and preservation of cellular membranes, aspects now recognized as "apoptotic."
The appearance of mitochondrial pyknosis is associated with activity in Colo-205 cell lysates after herbimycin exposure and could therefore represent the morphological correlative of the "point of no return" during the apoptotic pathway. An example of apoptotic mitochondrial morphology resembling mitochondrial pyknosis was reported in cardiac sinus node myocytes in patients with long QT syndrome and defined as "pleomorphic micromitochondriosis" (48). In this study, mitochondrial pyknosis was also associated with mitochondrial proliferation. Cupler et al. reported mitochondrial proliferation and ultrastructural abnormalities similar to mitochondrial pyknosis to be the earliest detectable lesion in zivudine (AZT)- associated myopathy in HIV-infected patients (19). These mitochondrial abnormalities could be one of the mechanisms leading to muscular atrophy, characteristic of more advanced stages of AZT-associated myopathy, through apoptosis. A recent study by Reipert et al. also described mitochondrial proliferation as an early response to treatment of the hematopoietic stem cell line FDCP mix with the topoisomerase II inhibitor etoposide, which induced G2 arrest and apoptosis (95). The increase in mitochondrial mass in this model was not associated with mitochondrial pyknosis. The fact that in this study the mitochondria became targets for autolytic digestion suggests however that they may be defective.
Colo-205 cells showed increased ROS production after herbimycin treatment. This response was preceded by mitochondrial proliferation and loss of net mitochondrial transmembrane potential and peaked at 96 h along with maximal induction of apoptosis.
This sequence suggests a possible role of ROS as mediators of the apoptotic response to herbimycin. Mitochondria could be a main target of nonspecific damage through oxidative stress at the level of the outer and inner membranes (12, 44). This mechanism has been described for the elimination of mitochondria during the physiologic maturation of reticulocytes into erythrocytes, which do not contain mitochondria. Reticulocytes eliminate these organelles through the glycoprotein lipooxygenase, which causes a disruption of both mitochondrial membranes by oxidation of phospholipids in the outer and inner membranes (92, 105). As a consequence of oxidative membrane damage, membrane potential and permeability-barrier function are impaired, leading to further mitochondrial damage. Recently, oxidative damage to the mitochondrial membrane due to increased generation of ROS has been shown to play a role in apoptosis of thymocytes and bone marrow cells in a murine model of Down's syndrome (86). Similar ROS-mediated mitochondrial membrane damage has been detected as a possible cause of apoptosis in Down's syndrome human fetal cortical neurons (11). Mitochondria have also been implicated as a source of ROS during apoptosis. Reduced mitochondrial membrane potential has recently been shown to lead to increased generation of ROS and apoptosis in dexamethasone-primed murine splenocytes in vivo (127). Mitochondria are the main source of cellular ROS (9, 117).
Mitochondrial dysfunction could also be more specifically programmed or targeted. There is precedent for the activation or upregulation of specific agents of mitochondrial dysfunction in the mitochondrial uncoupling protein present in brown fat adipocytes (80). This protein is a membranous proton carrier that induces uncoupling of mitochondrial respiration from ATP production by introducing a proton-conducting pathway through the mitochondrial inner membrane, with consequent decrease in mitochondrial transmembrane potential, reduced ATP phosphorylation, and dissipation of energy as heat (96). The activity of a mitochondrial uncoupler could be particularly effective when its activation is linked to mitochondrial proliferation, with consequent accumulation of dysfunctional mitochondria and massive production of ROS. In agreement with this hypothesis, NAC, a compound previously shown to protect cells against damage associated with oxidative stress (3, 73, 74, 113) protected Colo-205 cells from herbimycin-induced apoptosis, without affecting herbimycin-induced differentiation. However, mitochondrial proliferation and pyknosis in response to herbimycin were also abolished by cotreatment with NAC, underlining the importance of unbalanced mitochondrial proliferation and dysfunction in the apoptotic response in Colo-205 cells. This unexpected result appears to provide evidence for a redox-sensitive factor that governs the mitochondrial proliferation response.
Another targeted mechanism for diminished mitochondrial function might involve the opening of the mitochondrial permeability transition pores (7, 46, 129). Mitochondrial permeability transition has been recently proposed as a critical early event in the apoptotic process (57, 70, 128).
It is also possible that mitochondria in herbimycin-treated Colo-205 cells are defective at a biosynthetic level.
An example of this mechanism is the mitochondrial abnormalities, mentioned above, in the zivudine (AZT)-induced
myopathy in HIV-positive patients (19). Zivudine induces
inhibition of the mitochondrial DNA polymerase , with
consequent mitochondrial DNA depletion, reduced synthesis of mitochondrial subunits, and mitochondrial dysfunction (2, 75, 110). Mitochondrial proliferation may function as a compensatory response (4) that, because of
the biosynthetic defect, is not sufficient to restore an adequate mitochondrial function and leads to an accumulation
of dysfunctional mitochondria (19). Another experimental
model showing the deleterious effect of interference with
mitochondrial biosynthesis was reported by Garden et al.
(31). Neurons of the neonatal chick cochlear nucleus undergo cell death after afferent deprivation via removal of
the cochlea, preceded by increased oxidative metabolism
and mitochondrial mass (47, 102). Inhibition of mitochondrial protein synthesis, by in vivo administration of chloramphenicol, increases cell death after deafferentation (31).
Vayssiere et al. have also shown that reduction in
m
during apoptosis can be associated with reduction in mitochondrial translation (123).
Cell-free assays for apoptosis enable testing of the contribution of individual subcellular components or fractions, exceeding the experimental possibilities using intact cells (62). In addition, the temporal dependence of apoptosis on specific components can be examined (25, 54, 65, 71, 79). Our results show that subcellular fractions enriched in mitochondria are required for the apoptotic activity of lysates from Colo-205 cells treated for 24 h with herbimycin. At later time points, latent apoptotic factors in the cytosol are activated, and a strong mitochondrial dependence is no longer observed. Therefore, the apoptotic program induced by herbimycin requires the early action of mitochondria. Previous studies have shown a requirement for mitochondria in cell-free assays of apoptosis using extracts from Xenopus oocytes (79) or hepatocytes or lymphoid cells triggered to undergo apoptosis (128).
We also found evidence for the early timing of mitochondrial changes in herbimycin-induced apoptosis by examining their relationship to PARP cleavage. Cleavage of PARP, a proteolytic target of the CPP32/Yama protease (81, 115), has been described as an early event in drug-induced and other models of apoptosis in mammalian cells (25, 29, 51, 63, 106). Our results indicate that morphological and functional mitochondrial changes, as well as increase in ROS production, occur before detectable PARP cleavage. A similar result was reported in a recent study of cell death induced by respiratory chain inhibitors, etoposide or calcium ionophore (109).
An important aspect to consider in defining the role of mitochondria in the process of apoptosis is the anti-apoptotic proto-oncogene bcl-2, which encodes a protein localized in the outer mitochondrial membrane as well as endoplasmic reticulum and nuclear membrane (56, 97). Overexpression of Bcl-2 has been previously shown to suppress lipid peroxidation and ROS generation as well as prevent mitochondrial uncoupling (44, 50, 127). The Bcl-2 localization studies suggest that Bcl-2 may function directly at the mitochondrial membranes in these effects (42). Bcl-2 is not detectable in Colo-205 cells by Western blot (Mancini, M., and B. Anderson, unpublished result), but other members of this family may function as survival factors in Colo-205 cells. In addition to decreased expression or inhibitory posttranslational modifications, the protective effect of Bcl-2-like proteins could be diluted out by increased mitochondrial mass. Alternatively, Bcl-2 function might be restricted to specific mitochondrial subsets.
Finally, the relationship between mitochondrial proliferation and cell cycle arrest may be important. Mitochondrial proliferation is associated with cell cycle arrest in G2/M in Colo-205 cells after herbimycin treatment. Cell cycle arrests in Saccharomyces cerevisiae and Schizosaccharomyces pombe are associated with continued mitochondrial replication, eventually leading to abnormal mitochondrial morphologies (78, 104). Mitochondrial proliferation may be a manifestation of continued cell growth dissociated from cell cycle regulation (58). Dissociation between cytoplasmic and nuclear cell cycle events has been described in CHO cells after treatment with multiple cytostatic agents (59). In this model, a linear relationship between increased cellular size after exposure to the drug and decreased viability has been shown. G2 may be a sensitive phase of the cell cycle for dissociation of cytoplasmic and nuclear events in Colo-205 cells.
In conclusion, herbimycin A induces a proliferation of dysfunctional mitochondria together with enterocytic differentiation in Colo-205 cells. These mitochondrial changes are preceded by cell cycle arrest and followed by apoptosis. The association of cell cycle arrest, mitochondrial abnormalities, and apoptosis may be a useful model to explain the linkage of terminal differentiation to apoptosis in numerous cell lineages.
.
The authors are indebted to Andrew Berger for his excellent technical assistance in flow cytometry experiments and analysis of cell cycle data; to Judy Groombridge for the preparation of electron microscopy specimens; and to Paul Goodman, Paula Salewsky, and Tim Knight for their assistance with computer graphics and image analysis. We are grateful to Michael Wright and Stephen Schwartz for providing the rat liver nuclei preparation for in vitro assays of apoptosis.
m, mitochondrial membrane potential;
DHE, dihydroethidium;
H2-DCF-DA, dichlorodihydrofluorescein;
NAO, nonyl-acridine orange;
PARP, poly (ADP-ribose) polymerase;
ROS, reactive oxygen species;
TdT, terminal deoxynucleotidyl transferase.
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