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Address correspondence to Yuzhi Chen, MRC 223, McLean Hospital, 115 Mill St., Belmont, MA 02478. Tel.: (617) 855-3627. Fax: (617) 855-3793. email: ychen{at}helix.mgh.harvard.edu
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Abstract |
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Key Words: APP(V642I); NEDD8; secretase; lipid rafts; cell cycle
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Introduction |
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A subset of total APP is found on the cell surface in neurons (Jung et al., 1996), suggesting that a percentage of APP may function as a cell surface receptor, transducing signals from the ECM to the interior of the cell. Consistent with this notion, a number of proteins have been shown to interact with the COOH-terminal, intracellular portion of APP, among them the growth cone G protein Go (Nishimoto et al., 1993), the presumptive adaptor proteins Fe65 and X11 (for review see Russo et al., 1998), UV-DDB (Watanabe et al., 1999), histone acetyltransferase Tip60 (Cao and Sudhof, 2001), and Jun NH2-terminal kinase interaction protein 1 (Scheinfeld et al., 2002).
Previously, we identified an additional binding protein for the intracellular domain of APP, APP-BP1 (Chow et al., 1996). We showed that APP-BP1 is a cell cycle protein that acts in vivo as one half of the bipartite activating enzyme for the ubiquitin-like protein NEDD8 (Chen et al., 2000). Expression of APP-BP1 in dividing cells drives the cell cycle through the S-M checkpoint; this function is mediated by the NEDD8 conjugation (neddylation) pathway. In primary neurons, overexpression of APP-BP1 causes apoptosis; this apoptosis can be blocked by inhibition of neddylation (Chen et al., 2000).
Here, we dissect the nature of the interaction of APP-BP1 with APP, to determine whether disruptions in this interaction may play a role in AD neurodegeneration. We show that APP-BP1 binds to the COOH-terminal 31 aa of APP (C31), and that both proteins are located in lipid rafts. We demonstrate that APP, as well as APP-BP1, overexpression causes DNA synthesis and apoptosis in neurons, and that interaction of APP with APP-BP1 is required for activation of these pathways. Inhibition of neddylation blocks neuronal apoptosis caused not only by overexpression of APP-BP1 and APP but also by the V642I mutation of APP and by C31. Neurons expressing APP or APP(V642I) show an increase in APP-BP1 levels in lipid rafts, which is also observed in AD hippocampus. The increase in APP-BP1 is accompanied by a change in the subcellular localization of the ubiquitin-like protein NEDD8, which is activated by APP-BP1.
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Results and discussion |
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HSV-APPinduced apoptosis and DNA synthesis are blocked by coexpression of a peptide that competes with APP-BP1 for binding to APP
We have shown previously that either overexpression of wild-type (WT) APP or expression of FAD mutants of APP in neurons induces apoptosis and DNA synthesis (McPhie et al., 2001, 2003). To determine whether APP-BP1 interaction with APP is involved in either of these pathways, we used HSV vectors to coexpress in neurons a peptide representing the domain of APP-BP1 that binds to APP (Chow et al., 1996; Chen et al., 2000), together with WT APP or APP(V642I). Fig. 2 (A and B) shows that expression of this peptide, APP-BP1(145-251), blocks neuronal apoptosis and DNA synthesis caused by WT APP or APP(V642I), suggesting that interaction of APP with APP-BP1 is required for the activation of these pathways.
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Fig. 2 C illustrates the increase in DNA synthesis caused by APP(V642I), showing incorporation of BrdU into the DNA of neurons infected with HSV-APP(V642I). To confirm that the increase in DNA synthesis caused by APP(V642I) and blocked by APP-BP1(145-251) occurs specifically in neurons, we stained the neurons with a monoclonal anti-NeuN antibody, specific for neuronal nuclei, together with a polyclonal anti-BrdU antibody. As shown in Fig. 2 C (b), cells that are positively immunolabeled with anti-BrdU are colabeled with the antibody to NeuN, verifying their identity as neurons.
The fact that APP-BP1 apparently mediates APP-induced neuronal DNA synthesis is not unexpected, given our previous finding that APP-BP1 is necessary for cell cycle progression (Chen et al., 2000). However, in neurons this entry into the cell cycle causes apoptosis rather than cell cycle progression. The induction of DNA synthesis in neurons by overexpression of WT APP or of APP(V642I) is consistent with the data reported by Yang et al. (2001), who demonstrated that a significant number of hippocampal pyramidal and basal forebrain neurons in AD brain compared with control brain have undergone full or partial DNA replication.
APP-induced neuronal apoptosis is antagonized by disruption of the neddylation pathway that is initiated by APP-BP1
Overexpression of APP-BP1 in primary neurons causes apoptosis by a pathway that also involves hUbc12, the NEDD8-conjugating enzyme in the neddylation pathway initiated by APP-BP1. Coexpression of a dominant negative mutant (C111S) of hUbc12 with APP-BP1 prevents APP-BP1mediated neuronal apoptosis (Chen et al., 2000). We asked whether hUbc12(C111S) also blocks APP-induced apoptosis in neurons. As shown in Fig. 2 D, coexpression of hUbc12(C111S) with HSV-APP or with HSV-APP(V642I) reduces apoptosis induced by these viral recombinants nearly to control levels.
We suggested previously (McPhie et al., 2001) that APP C31 is responsible for the apoptosis caused by FAD mutants of APP. Therefore, we asked whether apoptosis caused by C31 is mediated by the neddylation pathway. Neurons in culture were infected with HSV vectors expressing GST-tagged C31 alone or in combination with hUbc12(C111S). The results (Fig. 2 D) confirm that C31 directly causes neuronal apoptosis, and that this apoptosis is inhibited by dominant negative hUbc12. Thus, activation of the neddylation pathway mediates neuronal apoptosis caused by APP-BP1, WT APP, APP(V642I), and C31.
To ascertain whether the dominant negative mutant of hUbc12 has a general effect on apoptotic pathways, we tested the effect of expression of hUbc12 dominant negative mutant on neurons treated with camptothecin, a topoisomerase inhibitor that activates the G1 to S transition in neurons and causes neuronal apoptosis (Park et al., 1997). Camptothecin induced a significant increase in DNA fragmentation in cortical cells; this effect peaked at 18 h (Fig. 2 E). This increase in DNA fragmentation was not prevented by coinfection with HSV expressing the dominant negative mutant of hUbc12 (Fig. 2 E). These data suggest either that camptothecin acts in a distinct apoptotic pathway or that its effects on apoptosis are distal to the effect of APP-BP1 in the same pathway.
The data above support the idea that APP-BP1 interaction with APP signals cells to divide via the neddylation pathway, causing differentiated neurons to die.
Expression of FAD APP(V642I) in neurons causes increased expression of APP-BP1 in the Triton X-100insoluble fraction
We have demonstrated that overexpression of APP-BP1 causes apoptosis in neurons. These data suggest that WT or FAD APPs may initiate the apoptotic pathway at least partly by increasing the level of APP-BP1 in neurons. To test this hypothesis, levels of APP-BP1 were assayed in lysates of neurons that had been infected with HSV-APP or HSV-APP(V642I) (Fig. 3 A). When primary cortical cultures were infected with these recombinant viruses, the level of the endogenous APP-BP1 in the Triton X-100insoluble/SDS-soluble fraction (lipid rafts) was increased many fold over basal levels. Expression of WT APP in neurons caused an intermediate increase in APP-BP1 levels in lipid rafts, consistent with our previous observation that overexpression of APP in neurons causes a level of DNA synthesis and apoptosis intermediate between that caused by APP(V642I) and that seen in control cultures. Camptothecin did not cause an increase of APP-BP1 in lipid rafts (Fig. 3 B); in fact, it caused a decrease, suggesting again that it acts by a different pathway from that activated by APP. No differences in total cellular APP-BP1 levels were detected in the samples shown in Fig. 3 (A and B; not depicted). These data suggest that WT APP and APP(V642I) cause the activation of cell cycle machinery and consequent apoptosis in neurons by increasing APP-BP1 levels specifically in lipid rafts.
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Immunofluorescent staining of Triton X-100treated hippocampal tissue sections with BP339 (Fig. 3 D) showed increased APP-BP1 in pyramidal cells in the hippocampus, supporting the immunoblot results. Brodmann areas 9 and 17, less pathologically affected in AD, were also analyzed by immunoblot, and did not show elevated levels of APP-BP1 over those in the same regions of control brain (Fig. 3 E). We did not observe increased APP-BP1 protein levels in the hippocampus of Parkinson's disease brains relative to controls (Fig. 3 F).
NEDD8 is translocated to the cytoplasm in hippocampal neurons in AD brain and in cultured neurons overexpressing APP-BP1
NEDD8 is a small ubiquitin-like protein that is activated by APP-BP1/hUba3 and covalently conjugated to target proteins such as the cullins. During most phases of the cell cycle, NEDD8 is located predominantly in the nucleus, but as mitosis begins, it moves to a predominantly cytoplasmic location (Kurz et al., 2002). Given that WT APP and APP(V642I) cause neuronal DNA synthesis that is mediated by APP-BP1, we predicted that NEDD8 may show a shift from a neuronal to a cytoplasmic localization in affected regions of AD brain. Indeed, we did observe such a shift (Fig. 4 A). We immunostained the hippocampus in five cases of AD and five matching controls with rabbit anti-NEDD8. In four out of five AD cases examined, NEDD8 was located primarily in the cytoplasm in hippocampal neurons. In contrast, in four out of five control cases, NEDD8 was located primarily in the nucleus in hippocampal neurons. Although the postmortem interval average for the control cases was slightly longer than that for the AD cases (average of 20 ± 1.28 h vs. 15 ± 1.96 h), there was no correlation between postmortem interval and NEDD8 localization in the cases that were examined. These data reinforce the notion that neurons in AD brain may be entering the cell cycle, and support the observation by Yang et al. (2001) that a significant number of hippocampal pyramidal and basal forebrain neurons in AD brain compared with control brain have undergone full or partial DNA replication.
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Conclusions
In summary, APP-BP1 interacts with APP within the COOH-terminal 31 aa of APP, and the two proteins coexist in lipid rafts. Induction of apoptosis or DNA synthesis in neurons by overexpression of wild type APP or APP(V642I) depends on the interaction of APP with APP-BP1 and is blocked by inhibition of neddylation. The levels of endogenous APP-BP1 protein in lipid rafts are both increased by APP or APP(V642I) overexpression in primary neuronal cultures and also increased in AD hippocampus. This increase in APP-BP1 levels in lipid rafts is associated with the translocation of NEDD8 from the nucleus to the cytoplasm both in AD hippocampal neurons and in primary neuronal cultures.
Our data suggest that neuronal apoptosis induced by overexpression of APP is mediated by its interaction with APP-BP1 in lipid rafts, which activates the neddylation pathway and induces cell cycle entry. It has been reported previously that the AICD, to which APP-BP1 binds, causes neuronal apoptosis via its effects on transcription (Kinoshita et al., 2002). One clue to the connection between APP-BP1 and the transcriptional pathway activated by the AICD is suggested by the finding that activation of NF-B inhibits the transcriptional activity of AICD (Zhao and Lee, 2003). We have shown that the neddylation pathway inhibits NF-
B activation (Chen et al., 2003). One may infer from these findings that activation of the neddylation pathway by APP-BP1 antagonizes the inhibition of the transcriptional activity of AICD by NF-
B, resulting in increased AICD transcriptional activity.
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Materials and methods |
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Plasmid construction
All plasmid constructs were made in the pHSVPrpUC vector or in the pcDNA3 vector using standard techniques, and were verified by sequence analysis. The APP-BP1 and dominant negative hUbc12 HSV vectors have been described previously (Chen et al., 2000), as have been the APP-695, APP(V642I), and myc-C31 HSV vectors (McPhie et al., 1997, 2001).
Antibodies and immunoblots
The polyclonal antibody 369, raised against amino acids 645694 of human APP695, was a gift from S. Gandy (Thomas Jefferson University, Philadelphia, PA). The anti-myc antibody 9E10 and the anti-APP antibody 22C11 were both obtained from CHEMICON International, Inc. or Upstate Biotechnology. The antiAPP-BP1 antibody BP339 has been described previously (Chen et al., 2000). The other antibodies used include: rabbit polyclonal anti-BrdU (Megabase Research Products); mouse monoclonal anti-NeuN (CHEMICON International, Inc.); Cy5-conjugated secondary (Jackson ImmunoResearch Laboratories); 10 µg/ml of rabbit anti-NEDD8 (Qbiogene or Zymed Laboratories); and myc antibody 9E10 (1:400; CHEMICON International, Inc.). HRP-conjugated cholera toxin, propidium iodide and (S)-(+)-camptothecin all were obtained from Sigma-Aldrich. Immunoblots were performed as described previously (Chen et al., 2000).
Coimmunoprecipitation
Rat primary neurons were infected with HSV-APP-BP1 and HSV myc-C31 for 4 h. Coimmunoprecipitation was performed as described previously (McPhie et al., 2003). The lysate was incubated with the anti-myc antibody 9E10 overnight and then with protein G for 2 h. The immune complex was resolved by SDS-PAGE, and subjected to immunoblot analysis with antiAPP-BP1 antibody BP339.
Apoptosis and DNA synthesis assays
For apoptosis assays, primary cortical cultures were infected with the appropriate viruses at an multiplicity of infection of one, and 14 h later, the cells were fixed for 20 min with 4% PFA and stained with bisbenzimide as described previously (McPhie et al., 2001). 10 random fields of 100200 cells were analyzed for each condition. The number of cells with condensed nuclei relative to the total number of cells per field was calculated and expressed as a percentage. All experiments were repeated three to four times. Both the DNA synthesis assays and the statistical analyses have been described in detail in Chen et al. (2003).
Immunocytochemistry with postmortem human brain sections and with ethanol-fixed primary neurons
40-µm-thick postmortem brain tissues fixed in PFA (Table I; Harvard Brain Tissue Resource Center) were immersed in TBS (20 mM Tris, pH 7.4, and 8.75% NaCl), rinsed three times with 10 mM phosphate buffer, and twice with diluting buffer (10 mM phosphate containing 2% BSA, 0.4% Triton X-100, and 1% normal goat serum). The tissue sections were incubated in 20% normal goat serum in 10 mM phosphate buffer for 10 min, after which they were incubated with rabbit antiAPP-BP1, BP339, or with rabbit anti-NEDD8 at room temperature for 40 h. Sections were washed once in 10 mM phosphate buffer and twice in diluting buffer. The sections were incubated with Alexa Fluor goat antirabbit overnight and washed before they were mounted onto slides.
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Acknowledgments |
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This work was supported by National Institutes of Health grant AG12954 to R.L. Neve. Y. Chen is an American Health Assistance Foundation Investigator for Alzheimer's Disease Research.
Submitted: 1 April 2003
Accepted: 28 August 2003
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