Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
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Abstract |
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-Catenin and plakoglobin are homologous proteins that function in cell adhesion by linking cadherins to
the cytoskeleton and in signaling by transactivation together
with lymphoid-enhancing binding/T cell (LEF/TCF) transcription factors. Here we compared the nuclear translocation and transactivation abilities of
-catenin and plakoglobin in mammalian cells. Overexpression of each of the two
proteins in MDCK cells resulted in nuclear translocation and formation of nuclear aggregates. The
-catenin-containing nuclear structures also contained LEF-1 and vinculin,
while plakoglobin was inefficient in recruiting these molecules, suggesting that its interaction with LEF-1 and vinculin is significantly weaker. Moreover, transfection of LEF-1
translocated endogenous
-catenin, but not plakoglobin to
the nucleus. Chimeras consisting of Gal4 DNA-binding domain and the transactivation domains of either plakoglobin or
-catenin were equally potent in transactivating a Gal4-responsive reporter, whereas activation of LEF-1-
responsive transcription was significantly higher with
-catenin. Overexpression of wild-type plakoglobin or mutant
-catenin lacking the transactivation domain induced
accumulation of the endogenous
-catenin in the nucleus and LEF-1-responsive transactivation. It is further shown
that the constitutive
-catenin-dependent transactivation in
SW480 colon carcinoma cells and its nuclear localization
can be inhibited by overexpressing N-cadherin or
-catenin.
The results indicate that (a) plakoglobin and
-catenin differ in their nuclear translocation and complexing with LEF-1
and vinculin; (b) LEF-1-dependent transactivation is preferentially driven by
-catenin; and (c) the cytoplasmic partners of
-catenin, cadherin and
-catenin, can sequester it to the cytoplasm and inhibit its transcriptional activity.
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Introduction |
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CELL-CELL adhesion plays an important role in tissue
morphogenesis and homeostasis (Gumbiner, 1996;
Huber et al., 1996a
), and is commonly mediated
by cadherins, a family of Ca2+-dependent transmembrane
adhesion receptors (for review see Geiger and Ayalon,
1992
; Kemler, 1992
; Takeichi, 1991
, 1995
). Cadherins were
shown to form homophilic interactions with similar receptors on neighboring cells, whereas their cytoplasmic domains interact with the cytoskeleton. The latter interactions are essential for stable adhesion and are mediated
via
-catenin, or its closely related homologue
-catenin
(plakoglobin), which interacts with microfilaments through
-catenin (Kemler, 1993
; Geiger et al., 1995
; Jou et al.,
1995
; Rimm et al., 1995
) and
-actinin (Knudsen et al.,
1995
).
In addition to the direct role of -catenin/plakoglobin in
promoting adhesion, their homologue in Drosophila armadillo (Peifer and Weischaus, 1990), was also shown to be
involved in the wingless (wg) signaling pathway that regulates cell fate during development (Peifer, 1995
; Orsulic
and Peifer, 1996
). In Xenopus,
-catenin participates in
the wnt-signaling pathway that determines body axis formation (for review see Miller and Moon, 1996
; Gumbiner, 1995
, 1996
), and overexpression of
-catenin and plakoglobin in Xenopus embryos was shown to induce double
axis formation (Funayama et al., 1995
; Karnovsky and
Klymkowsky, 1995
; Merriam et al., 1997
; Miller and Moon
1997
; Rubenstein et al., 1997
). In cultured cells, wnt overexpression elicits adhesion-related responses and increased
levels of
-catenin and plakoglobin (Bradley et al., 1993
;
Hinck et al., 1994
; Papkoff et al., 1996
).
-Catenin levels are regulated by glycogen synthase kinase-3
and adenomatous polyposis coli (APC)1 tumor suppressor protein
(Papkoff et al., 1996
; Rubinfeld et al., 1996
; Yost et al.,
1996
) which are thought to target
-catenin for degradation by the ubiquitin-proteasome system (Aberle et al.,
1997
; Orford et al., 1997
; Salomon et al., 1997
). When
-catenin levels are high, it can associate with architectural
transcription factors of the lymphoid-enhancing binding
factor/T cell factor (LEF/TCF) family and translocate into
the nucleus (Behrens et al., 1996
; Huber et al., 1996b
; Molenaar et al., 1996
). In the nucleus, the
-catenin-LEF/
TCF complex activates transcription of LEF/TCF-responsive genes that are not yet known in mammalian cells
(Morin et al., 1997
), but have partially been characterized in Xenopus (Brannon et al., 1997
) and Drosophila (Riese
et al., 1997
; van de Wetering et al., 1997
).
Elevation of -catenin in colon carcinoma cells that express a mutant APC molecule (Powell et al., 1992
; Polakis,
1997
), or in melanoma where mutations in the NH2-terminal domain of
-catenin were detected (both inhibiting
-catenin degradation) is oncogenic, most probably due to
constitutive activation of target genes which contributes to
tumor progression (Korinek et al., 1997
; Morin et al., 1997
;
Peifer, 1997
; Rubinfeld et al., 1997
). Interestingly, plakoglobin was shown to suppress tumorigenicity when overexpressed in various cells (Simcha et al., 1996
; Ben-Ze'ev,
1997
), and displays loss of heterozigosity in sporadic ovarian and breast carcinoma (Aberle et al., 1995
). Moreover,
upon induction of plakoglobin expression in human fibrosarcoma and SV-40-transformed 3T3 cells
-catenin is displaced from its complex with cadherin and directed to degradation (Salomon et al., 1997
).
In the present study we characterized the mechanisms
underlying nuclear accumulation of -catenin and/or plakoglobin and identified some of the partners associated
with both proteins in the nucleus. Furthermore, we compared the nuclear translocation and transactivation abilities of wild-type (wt) and mutant
-catenin and plakoglobin constructs and found that these two proteins differ
considerably in these properties, and demonstrated that
N-cadherin, as well as
-catenin, can drive
-catenin from
the nucleus to the cytoplasm and consequently block activation of LEF-1-responsive transcription. We propose
that the deregulated transactivation associated with elevated
-catenin in certain tumors can be suppressed by
cadherins and
-catenin.
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Materials and Methods |
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Cell Culture and Transfections
Canine kidney epithelial cells MDCK, human fibrosarcoma HT1080, 293-T
human embryonic kidney cells, Balb/C mouse 3T3, and human colon carcinoma SW480 cell lines were cultured in DME plus 10% calf serum
(Gibco Laboratories, Grand Island, NY) at 37°C in the presence of 7%
CO2. The human renal carcinoma cell line KTCTL60 (Simcha et al., 1996)
was grown in RPMI medium and 10% calf serum. Cells were transiently
transfected with the cDNA constructs described below, using Ca2+-phosphate or lipofectamine (Gibco Laboratories) and the expression of the
transgene was assessed between 24 and 48 h after transfection. In some
experiments, the expression of the stably transfected NH2 terminus-
deleted
-catenin (
57) (Salomon et al., 1997
) was enhanced in HT1080
cells by overnight treatment with 2 mM sodium butyrate.
Construction of Plasmids
The -catenin, plakoglobin, and
-catenin constructs were cloned in
frame into the pCGN expression vector containing a 16-amino acid (aa)
hemaglutinin (HA) tag at the NH2 terminus (see Fig. 1; Tanaka and Herr,
1990
). HA-
-catenin was obtained by PCR amplification of the 5' end of
mouse
-catenin (Butz et al., 1994) using oligonucleotides 5'-ACCTTCTAGAATGGCTACTCAAGCTGACCTG-3' and 5'-ATGAGCAGCGTCAAACTGCG-3'. The XbaI/SphI fragment of the PCR product
and the SphI/BamHI fragment of mouse
-catenin were joined and cloned
into the pCGN vector. A
-catenin mutant containing armadillo repeats
1-10 (see Fig. 1, HA
-catenin 1-ins) was obtained using oligonucleotides
5'-ACCTTCTAGATTGAAACATGCAGTTGTCAATTTG-3' and 5'-ACCTGGATCCAGCTCCAGTACACCCTTCG-3'. The amplified fragment was cloned into pCGN as an XbaI/BamHI fragment. Vesicular stomatitis virus (VSV)-tagged
-catenin at the COOH terminus was obtained by PCR amplification of the 3' end of Xenopus
-catenin (McCrea et al.,
1991
) using 5'-AACTGCTCCTCTTACTGA-3' and 5'-TATCCCGGGTCAAGTCAGTGTCAAACCA-3'. An XbaI/SmaI fragment of the amplified product was joined to an XbaI/EcoRI digest of Xenopus
-catenin
from Bluescript and then cloned into the pSY-1 plasmid containing an 11-aa
VSV glycoprotein tag (Kreis, 1986
) at the COOH terminus. The product
was subcloned into pCI-neo (Promega Corp., Madison, WI).
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HA-tagged human plakoglobin (see Fig. 1, HA plakoglobin) was obtained by PCR amplification of the 5' end of human plakoglobin cDNA
(pHPG 5.1) (Franke et al., 1989) using 5'-ACCTTCTAGAATGGAGGTGATGAACCTGATGG-3' and 5'-AGCTGAGCATGCGGACCAGAGC-3' oligonucleotide primers. The XbaI/SphI fragment of the PCR
product and the SphI/BamHI fragment of human plakoglobin cDNA
were cloned into the pCGN vector. A plakoglobin mutant containing
armadillo repeats 1-10 (see Fig. 1, HA plakoglobin 1-ins) was amplified
using 5'-ACCTTCTAGACTCAAGTCGGCCATTGTGC-3' and 5'-ACCTGGATCCTGCTCCGGTGCAGCCCTCC-3' oligonucleotides. The amplified fragment was cloned into the XbaI/BamHI site of pCGN. A
COOH terminus VSV-tagged plakoglobin (see Fig. 1, plakoglobin-VSV) was obtained by amplifying the 3' terminus of plakoglobin cDNA using
5'-AGGCCGCCCGGGCAGCATG-3' and 5'-CGCATGGAGATCTTCCGGCTC-3' oligonucleotide primers. The EcoRI/BglII fragment of the
plakoglobin cDNA and the BglII/SmaI fragment of the amplified product
were cloned in frame with the COOH terminus VSV tag into the pSY-1 plasmid. VSV-tagged plakoglobin was recloned into the pCI neoexpression vector (Promega Corp.) as an EcoRI/NotI fragment.
HA-tagged -catenin (see Fig. 1, HA
-catenin) was constructed by
amplification of the 5' end of chicken
-catenin (Johnson et al., 1993
) using 5'-ACCTTCTAGAATGACGGCTGTTACTGCAGG-3' and 5'-GCCTTCTTAGAGCGCCCTCG-3' oligonucleotide primers. The XbaI/ApaI
fragment of the PCR product and the ApaI/KpnI fragment of chicken
-catenin from pLK-
-catenin were cloned into the pCGN vector. An
HA-tagged mutant
-catenin (see Fig. 1, HA
-catenin
) lacking the
-catenin binding site (aa 118-166) was obtained by PCR-based mutagenesis using two fragments from the 5' end of
-catenin corresponding to aa
1-117 and 167-303, and oligonucleotides 5'-ACCTTCTAGAATGACGGCTGTTACTGCAGG-3', 5'-TGCCAGCGGAGCAGGGGTCATCAGCAAAC-3', 5'-CTGCTCCGCTGGCACCGAGCAGGATCTG-3' and
5'-ACGTTGCTCACTGAAGGTCG-3'. The two fragments were joined,
and the product was amplified using 5'-ACCTTCTAGAATGACGGCT-GTTACTGCAGG-3' and 5'-ACGTTGCTCACTGAAGGTCG-3' primers. The XbaI/BamHI fragment of the mutant construct and the BamHI/
KpnI fragment of chicken
-catenin from HA-
-catenin were cloned into
the pCGN vector. HA-tagged LEF-1 (Huber et al., 1996b
) at the COOH
terminus was a gift of R. Kemler (Max Planck Institute, Freiburg, Germany).
The DNA-binding domain of Gal4 (Gal4DBD) was obtained by PCR
using a 5'-ACCTTCTAGAATGAAGCTACTGTCTTCTATC-3' oligonucleotide with an XbaI site, and 5'-ACCTGAGCTCCGATACAGTCAACTGTCTTTG-3' with a SacI site (see Fig. 1, Gal4DBD -catenin
and Gal4DBD plakoglobin), and with the antisense primer 5'-ACCTGGATCCTACGATACAGTCAACTGTCTTTG-3' creating a BamHI site
(see Fig. 1, Gal4DBD). Fragments corresponding to the COOH terminus
of
-catenin (aa 682-781) and plakoglobin (aa 672-745) were obtained by
PCR using sense primers containing a SacI site and antisense primers containing a BamHI site. The XbaI/SacI fragment of Gal4DBD and the SacI/
BamHI fragments of
-catenin and plakoglobin were joined and cloned
into pCGN (see Fig. 1). The XbaI/BamHI fragment of Gal4DBD was
cloned into pCGN and used as control in transactivation assays. A cytomegalovirus promoter-driven N-cadherin cDNA was used (Salomon et
al., 1992). The validity of the constructs shown in Fig. 1 was verified by sequencing and then the size of the proteins were determined after transfection into 293-T and MDCK cells by Western blotting with anti-HA and
anti-VSV antibodies.
Transactivation Assays
Transactivation assays were conducted with SW480 and 293-T cells grown
in 35-mm-diam dishes that were transfected with 0.5 µg of a plasmid containing a multimeric LEF-1 consensus-binding sequence driving the luciferase reporter gene (TOPFLASH), or a mutant inactive form (FOPFLASH, provided by H. Clevers and M. van de Wetering, University of
Utrecht, Utrecht, The Netherlands). A plasmid encoding -galactosidase
(0.5 µg) was cotransfected to enable normalization for transfection efficiency. The relevant plasmid expressing catenin constructs (4.5 µg) was
cotransfected with the reporter, or an empty expression vector was included. After 24-48 h, expression of the reporter (luciferase) and the control (
-galactosidase) genes were determined using enzyme assay systems
from Promega Corp.
The Gal4RE reporter plasmid for determining transactivation by
Gal4DBD chimeras was constructed as follows: oligonucleotides comprising
a dimer of 17 nucleotides of the Gal4 binding sequences (Webster et al.,
1988) containing a SacI site at the 5' end and a BglII site at the 3' end, were
obtained by PCR amplification using 5'-GGAAGACTCTCCTCCGGATCCGGAAGACTCTCCTCC-3' and 5'-GATC-GGAGGAGAGTCTTCCGGATCCGGAGGAGAGTCTTCCAGCT-3' and subcloned as a SacI/
BglII fragment into the pGL3-promoter plasmid (Promega Corp.) driving
luciferase expression.
Northern Blot Hybridization
Total RNA was extracted from cells by the guanidinium thiocyanate
method. Northern blots containing 20 µg per lane of total RNA were
stained with methylene blue to determine the positions of 18S and 28S
rRNA markers, and then hybridized with plakoglobin (Franke et al.,
1989) and
-catenin (Butz et al., 1992
) cDNAs, which were labeled with
32P-dCTP by the random priming technique, as described in Salomon et al.
(1997)
.
Protease Inhibitors
The calpain inhibitor N-acetyl-leu-leu-norleucinal (ALLN, used at 25 µM) and the inactive analogue N-acetyl-leu-leu-normethional (ALLM, used at 10 µg/ml) were purchased from Sigma Chemical Co. (St. Louis, MO). Lactacystin A (dissolved in water at 0.4 mg/ml was used at a final concentration of 4 µg/ml) and MG-132 (used at 10 or 20 µM) were purchased from Calbiochem-Novabiochem (La Jolla, CA).
Immunofluorescence Microscopy
Cells cultured on glass coverslips were fixed with 3.7% paraformaldehyde
in PBS and permeabilized with 0.5% Triton X-100 (Sigma Chemical Co.).
Monoclonal antibodies against human plakoglobin (11E4), -catenin
(5H10), the COOH terminus of
-catenin (6F9), and
-catenin (1G5)
were described (Johnson et al., 1993
; Sacco et al., 1995
), and provided by
M. Wheelock and K. Johnson (University of Toledo, Toledo, OH). The
secondary antibody was FITC- or Cy3-labeled goat anti-mouse IgG
(Jackson ImmunoResearch Laboratories, West Grove, PA). Polyclonal
antiserum against
-catenin, monoclonal antibody against pan cadherin
(CH-19), vinculin (h-VIN 1), and
-actinin (BM75.2) were from Sigma
Chemical Co. (Holon, Israel). Monoclonal antibodies against the splicing
factor SC35 and the HA epitope were provided by D. Helfman (Cold
Spring Harbor Laboratory, Cold Spring Harbor, NY). Polyclonal rabbit
antibody against the VSV-G epitope was a gift of J.C. Perriard (Swiss Federal Institute of Technology, Zurich, Switzerland). FITC-labeled goat
anti-rabbit IgG antibody was from Cappel/ICN (High Wycombe, UK).
Monoclonal antibody against LEF-1 was provided by R. Grosschedl (University of California, San Francisco, CA). Polyclonal anti-HA tag antibody was a gift of M. Oren (Weizmann Institute, Rehovot, Israel). Antibodies to actin were provided by J. Lessard (Childrens Hospital Research Foundation, Cincinnati, OH) and I. Herman (Tufts University, Boston, MA). The cells were examined by epifluorescence with an Axiophot microscope (Carl Zeiss, Inc., Thornwood, NY). To determine the level of
overexpression relative to the endogenous protein, digitized immunofluorecent microscopy was used. Images of the fluorescent cells were recorded
with a cooled, scientific grade charge-coupled device camera (Photometrics, Tucson, AZ) as described (Levenberg et al., 1998). Integrated fluorescent intensities in transfected and nontransfected cells were determined after the background was substracted.
Electron Microscopy
Cells were processed for conventional electronmicroscopy by fixation
with 2% glutaraldehyde followed by 1% OsO4. The samples were dehydrated and embedded in Epon (Polysciences, Inc., Warington, PA), sectioned, and then examined in an electron microscope (model EM410;
Philips Electron Optics, Eindhoven, The Netherlands). Samples processed
for immunoelectronmicroscopy were fixed with 3% paraformaldehyde and 0.1% glutaraldehyde in 100 mM cacodylate buffer, pH 7.4, containing 5 mM CaCl2, embedded in 10% gelatin and refixed as above, and then incubated with sucrose, frozen, and then cryosectioned as previously described (Sabanay et al., 1991). The sections were incubated with monoclonal anti-vinculin (h-VIN-1), monoclonal anti-
-catenin (5H10), or
polyclonal anti-VSV tag (to recognize the VSV-tagged
-catenin), followed by secondary antibody conjugated to 10 nm gold particles (Zymed
Labs, Inc., South San Francisco, CA). The sections were embedded in
methyl cellulose and examined in an electron microscope (model CM12;
Philips Electron Optics).
PAGE and Immunoblotting
Equal amounts of total cell protein were separated by SDS PAGE, electrotransferred to nitrocellulose, and then incubated with monoclonal antibodies. The antigens were visualized by enhanced chemiluminescence (Amersham Int., Little Chalfont, UK). In some experiments, cells were fractionated into Triton X-100-soluble and -insoluble fractions as described (Rodríguez Fernández et al., 1992). Briefly, cells cultured on 35-mm dishes were incubated in 0.5 ml buffer containing 50 mM MES, pH 6.8, 2.5 mM EGTA, 5 mM MgCl2 and 0.5% Triton-X-100 at room temperature for 3 min. The Triton X-100-soluble fraction was removed, and the insoluble fraction was scraped into 0.5 ml of the same buffer. Equal volumes of the two fractions were analyzed by SDS-PAGE and followed by immunoblotting with the various antibodies.
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Results |
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Overexpression of -Catenin and Plakoglobin in
MDCK Cells Results in Their Nuclear Accumulation
and Nuclear Translocation of Vinculin
To determine the localization of overexpressed -catenin
and plakoglobin, MDCK cells that normally display these
molecules at cell-cell junctions were transiently transfected with VSV-tagged
-catenin or plakoglobin cDNA
constructs (Fig. 1) and immunostained with either anti-VSV, anti-
-catenin, or antiplakoglobin antibodies. The
results in Fig. 2 show that when expressed at very low
level,
-catenin was detected at cell-cell junctions (Fig. 2
F), but in cells expressing higher levels (approximately
fivefold over the endogenous protein level, as determined
by digital immunofluorescent microscopy), most of the
transfected molecules were localized in the nuclei of cells,
either in a diffuse form, or in aggregates of various shapes
(Fig. 2, A-C, speckles and rods). These
-catenin-containing aggregates were organized in discernible structures
that could be identified by phase microscopy (Fig. 3, E and
F). Transmission electron microscopy of Epon-embedded
cells revealed within the nucleus highly ordered bodies
consisting of laterally aligned filamentous structures, with
a filament diameter of ~10 nm and packing density of ~50
filaments/µm (Fig. 3 A). Immunogold labeling of ultrathin
frozen sections indicated that these intranuclear bodies
contained high levels of
-catenin (Fig. 3, B and C).
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Transfection of plakoglobin also resulted in nuclear accumulation of the molecule and, in addition, showed diffuse cytoplasmic (Fig. 2 D) and junctional staining (see below, Fig. 7 H). Whereas nuclear aggregates were observed
in plakoglobin-transfected cells (Fig. 2 E), large rods were
not detected in the nuclei of these cells. Similar structures
were observed with HA-tagged and untagged -catenin or
plakoglobin (data not shown), suggesting that these structures in the nucleus assembled due to high levels of these proteins and are not attributable to tagging.
|
The unique assembly of -catenin into discrete nuclear
structures is, most probably nonphysiological, yet it enabled us to examine the association of other molecules
with
-catenin (Fig. 4). Interestingly, in addition to the
transcription factor LEF-1 (Fig. 4 B) that was shown to
complex with
-catenin in the nucleus (Behrens et al.,
1996
; Huber et al., 1996b
; Molenaar et al., 1996
), vinculin
also strongly associated with the
-catenin-containing speckles and rods in the nucleus (Fig. 4, C and D). This
was also confirmed by immunogold labeling of ultrathin
frozen sections with anti-vinculin antibodies showing that
the labeling was distributed throughout the entire nuclear
aggregate (Fig. 3 D). In contrast, other endogenous proteins known to be involved in linking cadherins to actin at
adherens junctions such as
-catenin (Fig. 4 F),
-actinin (Fig. 4 J), and plakoglobin (Fig. 4 H) were not associated
with the
-catenin-containing nuclear aggregates. Actin
was also missing from these nuclear aggregates (results not
shown). Furthermore, the
-catenin-containing rods and
speckles were clearly distinct from other nuclear structures such as the splicing component SC35 (data not
shown), which also displays a speckled nuclear organization in many cells (Cáceres et al., 1997
).
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The molecular interactions of plakoglobin were distinctly different from those formed by -catenin. Although
nuclear speckles in MDCK cells overexpressing plakoglobin displayed some faint staining for LEF-1 (Fig. 5, compare B with A), this was less pronounced than that seen
with
-catenin (Fig. 4 B), and essentially no nuclear costaining for vinculin,
-catenin (data not shown), or
-actinin (Fig. 5 F) was observed. Interestingly, plakoglobin-containing nuclear speckles (Fig. 5 C) were also stained
with anti-
-catenin, and the cytoplasm of these cells was
essentially devoid of the diffuse
-catenin staining seen in
nontransfected cells (Fig. 5 D). This may be explained by
the capacity of plakoglobin to compete and release
-catenin from its other partners (i.e., cadherin or APC) leading
to its nuclear translocation.
|
Nuclear Accumulation of -Catenin after
Induced Overexpression
Transient transfection usually results in very high and nonphysiological levels of expression (and organization) of
the transfected molecules. To obtain information on
-catenin that is more physiologically relevant, we have
isolated HT1080 cells stably expressing a mutant
-catenin
molecule lacking the NH2-terminal 57 aa (
N57, Salomon et al., 1997
) that is considerably more stable than the wt
protein (Munemitsu et al., 1996
; Papkoff et al., 1996
; Rubinfeld et al., 1996
; Yost et al., 1996
). In such stably transfected cells, the level of expression was low and only faint
nuclear
-catenin staining was detected, with the majority
of
-catenin localized at cell-cell junctions (Fig. 6 C).
However, when the cells were treated with butyrate, an
approximate twofold increase in the expression of the
transgene was observed by Western blot analysis (data not shown), and a dramatic translocation of
-catenin into the
nucleus occurred (Fig. 6 D). This translocation was not observed in butyrate-treated control neor HT1080 cells (Fig.
6 B). These results suggest that an increase in
-catenin
over certain threshold levels of either transiently or inducibly expressed
-catenin results in its nuclear translocation and accumulation.
|
LEF-1 Overexpression Induces Translocation of
Endogenous -Catenin but Not Plakoglobin into the
Nuclei of MDCK Cells
Nuclear translocation of -catenin was shown to be promoted by elevated LEF-1 expression. We have thus compared the ability of transfected LEF-1 to induce the translocation of endogenous
-catenin and plakoglobin into the
nuclei of MDCK cells. Cells were transfected with an HA-tagged LEF-1 and after 36 h were doubly immunostained
for LEF-1 and
-catenin, or for LEF-1 and plakoglobin. As
shown in Fig. 7, whereas endogenous
-catenin was efficiently translocated into the nucleus in LEF-1-transfected
cells and colocalized with LEF-1 (Fig. 7, A and B), plakoglobin was not similarly translocated into the nucleus (Fig.
7, compare D with C). This difference between
-catenin
and plakoglobin could result from the larger pool of diffuse
-catenin (Fig. 8 A) that is available for complexing and
translocation into the nucleus with LEF-1. In contrast, plakoglobin was almost exclusively found in the Triton X-100-
insoluble fraction (Fig. 8 A), in association with adherens
junctions and desmosomes. Vinculin and
-catenin that also
display a large Triton X-100-soluble fraction (Fig. 8 A), in
contrast to
-actinin and plakoglobin, were not translocated
into the nucleus after LEF-1 transfection (data not shown).
|
When LEF-1 was overexpressed in MDCK cells together
with plakoglobin, both proteins were localized in the nucleus and displayed diffuse staining (Fig. 7, G and H), similar to -catenin (Fig. 7, E and F). In cells doubly transfected
with
-catenin and LEF-1, vinculin was also translocated
into the nucleus displaying diffuse staining (Fig. 8 B, panels
a and b), whereas plakoglobin was not detected in the nuclei of such cells (Fig. 8 B, panels c and d). Since LEF-1
transfection did not result in nuclear localization of vinculin
(data not shown), this implies that vinculin translocation into the nucleus is only related to that of
-catenin.
Induction of -Catenin Accumulation and
Its Nuclear Localization by Inhibition of the
Ubiquitin-Proteasome System
Another treatment through which -catenin and plakoglobin content could be elevated in cells is the inhibition of
degradation by the ubiquitin-proteasome pathway that
apparently controls
-catenin and plakoglobin turnover
(Aberle et al., 1997
; Orford et al., 1997
; Salomon et al.,
1997
). We have used two cell lines for this study: 3T3 cells
that express
-catenin, and KTCTL60 renal carcinoma cells that do not express detectable levels of cadherin,
-catenin,
-catenin, or plakoglobin (Simcha et al., 1996
),
and treated them with various inhibitors of the ubiquitin-
proteasome system (Fig. 9). In 3T3 cells, such treatment
resulted in the appearance of higher molecular weight
-catenin forms representing most likely ubiquitinated derivatives of the molecule (Fig. 9 A). This was accompanied
by the accumulation of
-catenin in the nuclei of the cells
(Fig. 9 D, compare panels a and b). In KTCTL60 cells that
contain minute levels of
-catenin, inhibitors of the ubiquitin-proteasome pathway induced a dramatic increase in
the level of
-catenin (Fig. 9 B), and its translocation to
the nucleus (Fig. 9 D, compare panels c and d). Since these
cells express no cadherins (Simcha et al., 1996
), it is conceivable that free
-catenin is very unstable and rapidly
degraded by the proteasome pathway in these cells. In
contrast, no plakoglobin was detected in KTCTL60 cells
either before or after treating the cells with the proteasome inhibitors (Fig. 9 B) due to lack of plakoglobin RNA
in these cells (Fig. 9 C). When plakoglobin was stably
overexpressed in KTCTL60-PG cells (Fig. 9 C), its level
was further increased by inhibitors of the ubiquitin-proteasome system (Fig. 9 B), and it accumulated in the nuclei
of the cells (Fig. 9 D, panels e and f). These results demonstrate that in some cells the level of
-catenin can be dramatically enhanced by inhibiting its degradation by the
ubiquitin-proteasome pathway. Plakoglobin levels were
also enhanced by MG-132 treatment, but to a considerably
lower extent. Under conditions of excess, both proteins accumulated in the nuclei of cells.
|
Transcriptional Coactivation by Plakoglobin and
-Catenin of Gal4- and LEF-1-driven Transcription
-Catenin and its homologue in Drosophila armadillo
were shown to be able to activate transcription of LEF/
TCF-responsive consensus sequences by their COOH termini (Korinek et al., 1997
; Morin et al., 1997
; Riese et al.,
1997
; van de Wetering et al., 1997
). To compare the ability
of
-catenin to that of plakoglobin in transactivation, the
COOH terminus of
-catenin and the corresponding domain in plakoglobin were fused to the Gal4DBD (refer to
Fig. 1). Both constructs, when cotransfected with a reporter gene (luciferase) whose transcription was driven by
a Gal4-responsive element, showed a similar ability to activate the expression of the reporter gene (Fig. 10 A). This
implies that the COOH-terminal domain of plakoglobin, like that of armadillo and
-catenin, has the ability to activate transcription.
|
Next, we compared the capacity of -catenin and plakoglobin to activate transcription of a reporter gene driven by
a multimeric LEF-1-binding consensus sequence in 293 cells. The results summarized in Fig. 10 B demonstrate that
-catenin is a potent transcriptional coactivator of the multimeric LEF-1-responsive sequence. Interestingly, a mutant
-catenin lacking the COOH transactivation domain (refer to Fig. 1, HA
-catenin 1-ins) was also active in promoting
LEF-1-driven transcription (Fig. 10 B). We examined
whether this resulted from the substitution for endogenous
-catenin in its complexes with cadherin and APC by the
mutant
-catenin as seen in Xenopus embryos injected with
mutant
-catenin (Miller and Moon, 1997
). This could release endogenous
-catenin from cytoplasmic complexes,
resulting in its translocation into the nucleus and transcriptional activation of the LEF-1-responsive reporter. Double
immunofluorescence using an antibody against the HA-tag
linked to the mutant
-catenin (Fig. 10 C, panel c) and an
anti-
-catenin antibody recognizing the COOH terminus of
endogenous
-catenin (but not the mutant HA
-catenin 1-ins
that lacks this domain), demonstrated that the level of endogenous
-catenin was elevated, and part of the endogenous protein translocated into the nucleus in cells expressing mutant
-catenin (Fig. 10 C, panel d).
Plakoglobin could also activate LEF-1-driven transcription, albeit at a three- to fourfold lower extent than -catenin (Fig. 10 B). A mutant plakoglobin lacking the COOH
transactivation domain (refer to Fig. 1, HA plakoglobin
1-ins), was unable to enhance LEF-1-driven transcription
(Fig. 10 B). To examine if full-length or mutant plakoglobin overexpression resulted in nuclear accumulation of endogenous
-catenin, cells transfected with HA-tagged plakoglobin were doubly stained for HA (Fig. 10 C, panel a),
and
-catenin (Fig. 10 C, panel b). The results demonstrated that plakoglobin overexpression resulted in nuclear translocation of endogenous
-catenin (Fig. 10 C,
compare panel a with b). In contrast, the COOH deletion
mutant plakoglobin that was abundantly expressed in the
transfected cells (Fig. 10 C, panel e), was unable to cause translocation of endogenous
-catenin into the nucleus (Fig.
10 C, panel f).
Taken together, these results strongly suggest that although both plakoglobin and -catenin have a COOH-terminal domain that can act as cotranscriptional activator
when fused to the Gal4DBD, LEF-1-driven transcriptional
activation by mutant
-catenin and wild-type plakoglobin
mostly resulted from the release of endogenous
-catenin
from its cytoplasmic partners, nuclear translocation, and
induction of LEF-1-responsive transcription. Thus, elevated plakoglobin expression can influence
-catenin-driven
transactivation.
Inhibition of -Catenin Nuclear Localization and
Transactivation Capacity by N-Cadherin and
-Catenin
Constitutive transactivation by high levels of -catenin
was suggested to be involved in tumor progression in colon carcinoma (Korinek et al., 1997
; Morin et al., 1997
). In
addition, the signaling activity of
-catenin in Xenopus development could be blocked by its junctional partners (i.e.,
C-cadherin and the NH2-terminal of
-catenin [Funayama
et al., 1996; Sehgal et al., 1997
]). We have investigated the
localization and transcriptional activation capacity of
-catenin in SW480 colon carcinoma cells that overexpress
-catenin due to lack of APC (Munemitsu et al.,
1995
), before and after transfection with N-cadherin and
-catenin. In these cells,
-catenin is abundant in the nucleus (Fig. 11 B, panels b, d, and f) and a high level of constitutive LEF-1-driven transcription was detected (Fig.
11 A), in agreement with Korinek et al. (1997)
. This activity of
-catenin was effectively blocked by the cotransfection of N-cadherin or
-catenin (Fig. 11 A). Deletion of the
-catenin binding site on
-catenin (refer to Fig. 1,
-catenin
) abolished the transactivation inhibition capacity
of this molecule (Fig. 11 A). Double immunofluorescence
microscopy indicated that both molecules can drive
-catenin out of the nucleus in transfected SW480 cells (Fig. 11
B). In these cells,
-catenin was sequestered to the cytoplasm by
-catenin (Fig. 11 B, panels c and d) or to cell-
cell junctions by the transfected N-cadherin (Fig. 11 B, panels a and b). The
-catenin mutant lacking the
-catenin binding site (Fig. 11 B, panels e and f) did not show this
activity. The results suggest that the partners of
-catenin
that are active in cell adhesion, are effective antagonists of
the nuclear localization of
-catenin and its function in
transcriptional regulation.
|
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Discussion |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
In mammalian cells, -catenin and the closely related molecule plakoglobin (Butz et al., 1992
; Knudsen and Wheelock, 1992
; Peifer et al., 1992
) have been shown to complex
independently with similar partners, and are both involved
in the formation of adherens type junctions (Butz and
Kemler, 1994
; Hülsken et al., 1994
; Nathke et al., 1994
; Rubinfeld et al., 1995
). Plakoglobin, in addition, can associate
with various desmosomal components (Cowin et al., 1986
;
Schmidt et al., 1994
; Chitaev et al., 1996
; Kowalczyk et al.,
1997
), whereas
-catenin does not normally associate with
desmosomes, except in plakoglobin-null mouse embryos
where the segregation between adherens junctions and
desmosomes collapses (Bierkamp et al., 1996
; Ruiz et al.,
1996
). Plakoglobin is also unable to substitute for
-catenin during development, since
-catenin-null mouse embryos die early in development (Haegel et al., 1995
). In
this study we highlight some common features of
-catenin and plakoglobin, as well as considerable differences in
their nuclear translocation under various conditions, and
their capacity to function in transcriptional activation. For
both proteins, the increase in free protein levels induces
nuclear translocation. This translocation can be blocked
by junctional proteins that bind to
-catenin and sequester
it to the plasma membrane or the cytoplasm.
Nuclear Translocation
Under the various conditions that resulted in increased levels of -catenin and plakoglobin, both proteins translocated into the nucleus independently of, or in complex with
LEF-1. Whereas in
-catenin-overexpressing cells the nuclear complexes that were formed by excess
-catenin also
contained vinculin in addition to LEF-1, plakoglobin overexpression did not result in the recruitment of these molecules into the nuclear speckles. Interestingly,
-catenin and
-actinin, both of which bind
-catenin and plakoglobin-containing complexes (Knudsen et al., 1995
; Huber et al.,
1997
; Nieset et al., 1997
), were not cotranslocated into the
nucleus by
-catenin or plakoglobin probably due to their
stronger binding to actin filaments, resulting in a limited
soluble pool in cells, in contrast to vinculin that is mostly in
the detergent-soluble fraction. Our study is the first demonstration that
-catenin can associate with and recruit into
the nucleus vinculin, but not other components of the cadherin-catenin system (i.e.,
-catenin,
-actinin, or cadherin)
in a complex that also contains LEF-1. The association between vinculin and
-catenin was recently demonstrated
by coimmunoprecipitation of these proteins together with
E-cadherin, but was most pronounced in cells lacking
-catenin (Hazan et al., 1997
). Taken together, these findings reveal a new interaction of
-catenin with vinculin, that under certain conditions may lead to their colocalization in the nucleus where such a complex may play an important physiological role yet to be determined.
Although both plakoglobin and -catenin exhibited a
largely similar nuclear translocation, they were distinct in
their ability to colocalize with LEF-1 in the nucleus. Endogenous
-catenin was readily translocated into the nucleus after transfection with LEF-1, in agreement with previous studies (Behrens et al., 1996
; Huber et al., 1996b
;
Molenaar et al., 1996
), whereas the endogenous plakoglobin remained junctional. This difference may be attributed to the availability of a larger pool of soluble
-catenin in
MDCK cells, or to an intrinsic difference between the two
molecules in their binding to LEF-1.
Plakoglobin and -catenin also differed in their ability to
influence the localization of endogenous LEF-1 when individually overexpressed. Plakoglobin overexpression could
drive part of the endogenous
-catenin into the nucleus,
most probably by displacing it from cadherin or other cytoplasmic partners, in agreement with results obtained with
HT1080 cells (Salomon et al., 1997
) and with Xenopus embryos (Miller and Moon, 1997
). This implies that plakoglobin may have a regulatory role in the control of the extrajunctional function of
-catenin. In contrast,
-catenin was
inefficient in altering plakoglobin's localization in MDCK,
293, and SK-BR-3 cells (all expressing desmosomes, our
unpublished results). This was partly expected since
-catenin is not normally associated with desmosomes and the
soluble pool of plakoglobin in these cells is very low.
Plakoglobin and -catenin also responded differently to
the inhibition of the ubiquitin-proteasome pathway, in
particular in the renal carcinoma cell line KTCTL60 that
does not express detectable levels of proteins of the cadherin-catenin system (Simcha et al., 1996
). The level of
-catenin could be dramatically induced in these cells with
proteasome inhibitors, suggesting that efficient degradation of
-catenin is responsible for the very low level of
-catenin in these cells. Plakoglobin was absent from these cells since there was no plakoglobin RNA, but when it was
stably expressed (Simcha et al., 1996
), its level was only
moderately enhanced by inhibitors of the ubiquitin-proteasome system. It is interesting to note that this stable expression of plakoglobin did not result in the elevation of
-catenin content in KTCTL60 cells, implying that plakoglobin cannot, by itself, effectively protect
-catenin from
degradation in cells lacking cadherins. Only when the level
of plakoglobin was further increased in these cells by butyrate treatment could some accumulation of
-catenin be
detected (our unpublished results).
Transactivation Capacity
Comparison between the presumptive transactivating domains of -catenin and plakoglobin, fused to the DNA-binding domain of Gal4, indicated comparable transcriptional activation by the two molecules. This demonstrated
that plakoglobin, like
-catenin and armadillo (van de Wetering et al., 1997
), has a potent transactivation domain.
However, the specific transcriptional activation of LEF-1- driven reporter gene by plakoglobin was severalfold less
efficient than that of
-catenin. Interestingly, a deletion
mutant of
-catenin that lacked the transactivating domain but retained the cadherin-binding domain, was also
capable of inducing transcription by the LEF-1 consensus
construct. This can be attributed to competition and displacement of endogenous
-catenin from a complex with
its cytoplasmic partners, nuclear translocation of the endogenous
-catenin, and consequently, LEF-1-driven transactivation. This finding is in agreement with recent studies
by Miller and Moon (1997)
who found that a variety of
membrane-anchored mutant forms of
-catenin can act in
signaling for axis duplication in Xenopus embryos by releasing endogenous
-catenin from cell-cell junctions or
from a complex with APC, thus enabling its translocation
into the nucleus.
Overexpression of full-length plakoglobin was capable
of inducing nuclear translocation of the endogenous -catenin in MDCK and 293 cells, whereas a COOH terminus
mutant plakoglobin, previously shown to be inefficient in
displacing
-catenin from its complex with cadherin (Sacco
et al., 1995
; Salomon et al., 1997
), was also unable to induce nuclear localization of the endogenous
-catenin, or
transcriptional activation of the LEF-1-driven reporter.
Since plakoglobin overexpression was inefficient in driving LEF-1 to complex with the nuclear speckles formed by
plakoglobin overexpression, it is conceivable that the majority of the transcriptional stimulation of LEF-1-driven
transcription in plakoglobin-overexpressing cells was due
to the endogenous
-catenin that relocated to the nucleus under these conditions.
Another recent study examining mammalian -catenin
and plakoglobin's embryonic signaling abilities in Drosophila (rescue of the segment polarity phenotype of armadillo) suggested that although both proteins can rescue armadillo mutants in adhesion properties,
-catenin had
only a weak and plakoglobin had no detectable signaling activity (White et al., 1998
). Nevertheless, since we found
that the COOH terminus of plakoglobin is potent in transcriptional activation in the Gal4-fusion chimera and deletion mutants at the COOH terminus were inefficient in
transactivation and most of the overexpressed plakoglobin
was localized in the nuclei of transfected cells, one cannot
exclude the possibility that plakoglobin can also play a direct role in the transcriptional regulation of specific genes
that are yet to be identified (Rubenstein et al., 1997
). This
possibility is being currently examined by analyzing the
transactivation capacities of plakoglobin and
-catenin in cells that lack such endogenous proteins.
In the human colon carcinoma SW480 cells that lack
APC and therefore accumulate abnormally high levels of
-catenin in the nuclei, transcriptional activation of the
LEF-1-driven reporter could be inhibited by members of
the cadherin-catenin complex that sequestered
-catenin
to the cytoplasm. The role of cadherin in regulating
-catenin levels is complex. On one hand, elevation in the content
of cadherin can protect
-catenin from degradation and
increase its levels. On the other hand, a strong binding of
-catenin to cadherin, rather than to LEF-1, may result in
its cytoplasmic sequestration and the inhibition of transactivation by it. Furthermore, the cytoplasmic tail of cadherin that contains the binding site for
-catenin can also
inhibit transactivation by
-catenin even when bound to it in
the nucleus of the transfected cells (Sadot, E., M. Shtutman, I. Simcha, A. Ben-Ze'ev, and B. Geiger, unpublished
results). The association of
-catenin with overexpressed
-catenin in SW480 cells also resulted in the cytoplasmic retention of nuclear
-catenin by binding of
-catenin to the actin cytoskeleton. These results are in agreement with those obtained for
-catenin signaling in axis specification of developing Xenopus that is antagonized by overexpression
of cadherin (Heasman et al., 1994
; Fagotto et al., 1996
), or
by the NH2 terminus of
-catenin (Sehgal et al., 1997
).
These results may have important implications for the
possible role of -catenin in the regulation of tumorigenesis, since E-cadherin (Navarro et al., 1991
; Vleminckx et
al., 1991
) and
-catenin (Bullions et al., 1997
) were suggested to have tumor suppressive effects when reexpressed in cells deficient in these proteins and were shown
to affect the organization of cell-cell adhesion. In addition, modulation of vinculin and
-actinin levels in certain
tumor cells was shown to influence the tumorigenic ability
of these cells (Rodríguez Fernández et al., 1992; Glück et
al., 1993
) and to affect anchorage independence and tumorigenicity in 3T3 cells (Rodríguez Fernández et al., 1993;
Glück and Ben-Ze'ev, 1994
; Ben-Ze'ev, 1997
). It is possible that such effects are attributable to the capacity of vinculin and
-actinin to bind
-catenin, thus affecting both
its localization and its role in regulating transcription.
Since the -catenin-binding domains on E-cadherin
(Ozawa et al., 1990
; Stappert and Kemler, 1994
) and
-catenin (Bullions et al., 1997
; Huber et al., 1997
; Nieset et al.,
1997
; Obama and Ozawa, 1997
) are well characterized, it
will be possible to test in the future, whether shorter peptides comprising these domains are effective in blocking
the oncogenic action conferred by constitutive transactivation of LEF/TCF-responsive genes by
-catenin in colon cancer (Korinek et al., 1997
; Morin et al., 1997
) or melanoma (Rubinfeld et al., 1997
).
![]() |
Footnotes |
---|
Received for publication 19 February 1998 and in revised form 7 April 1998.
Address all correspondence to: Avri Ben-Ze'ev, Department of Molecular Cell Biology, Weizman Institute of Science, Rehovot 76100, Israel. Tel.: (972) 8-934-2422. Fax: (972) 8-934-4125. E-mail: lgbenzev{at}weizmann.weizmann.ac.ilWe are grateful to our colleagues for sending reagents: R. Kemler, R. Grosschedl, M. Wheelock, K. Johnson, D. Helfman, J.C. Perriard, M. Waterman (University of California, Irvine, CA), K. Jones (The Salk Institute, La Jolla, CA), H. Clevers, and M. van de Wetering; to I. Sabanay (The Weizmann Institute of Science) for electron microscopy and Z. Kam (The Weizmann Institute of Science) for helping with digital immunofluorescent microscopy.
These studies were supported by grants from the USA-Israel Binational Foundation, the German-Israeli Foundation for Scientific Research and Development, by the Forchheimer Center for Molecular Genetics, and by the Cooperation Program in Cancer Research between the German Cancer Research Center (DKFZ) and the Israeli Ministry of Sciences and Arts (IMOSA).
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Abbreviations used in this paper |
---|
aa, amino acid(s); APC, adenomatous polyposis coli; DBD, DNA-binding domain; HA, hemagglutinin; LEF, lymphoid-enhancing binding factor; TCF, T cell factor; VSV, vesicular stomatitis virus; wt, wild-type.
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