Article |
Address correspondence to David Pellman, Dana-Farber Cancer Institute/Harvard Medical School, Dept. of Pediatric Oncology, Rm. M612A, 44 Binney St., Boston, MA 02115. Tel.: (617) 632-4918. Fax: (617) 632-5757. E-mail: david_pellman{at}dfci.harvard.edu
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Abstract |
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Key Words: cell cycle; cyclin-dependent kinases; mitosis; S phase; ubiquitin
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Introduction |
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To coordinate a normal cell cycle, these two APC/C activities must be linked either directly or indirectly to cyclin-dependent kinase (CDK) activity. For APCCdh1, the linkage is thought to take place primarily through CDK phosphorylation of Cdh1p. In yeast, Cdh1p levels are constant throughout the cell cycle, but its binding to APC/C is blocked by CDK phosphorylation (Zachariae et al., 1998; Jaspersen et al., 1999). At the end of mitosis, APCCdh1 is activated because Cdh1p is dephosphorylated and binds APC/C. Both the drop in mitotic kinase activity and the activation of the phosphatase Cdc14p contribute to Cdh1p dephosphorylation during mitotic exit (Visintin et al., 1998; Shou et al., 1999). Eventually, APCCdh1 is inactivated in the next cell cycle by rising CDK activity. However, the precise timing of APCCdh1 inactivation and the CDK isoforms involved have not been determined clearly. The experimental evidence to date supports the view that G1 CDKs alone inactivate what is now known to be APCCdh1 (Amon et al., 1994). Additionally, the possibility that S phase cyclins could have a role in APCCdh1 inactivation has been raised because failure to degrade an S phase cyclin by the end of mitosis can block APCCdh1 function and mitotic exit (Shirayama et al., 1999; Zachariae and Nasmyth, 1999).
During G1, APCCdh1 activity is required to prevent expression of proteins that may interfere with bud emergence, lead to premature DNA replication, or disturb spindle assembly (Amon et al., 1994; Irniger and Nasmyth, 1997; Juang et al., 1997). Before the onset of the ensuing mitosis, APCCdh1 must be turned off in order to allow its mitotic substrates to accumulate. Several lines of evidence linked G1 CDK activity (Cln1p-Cln3p bound to Cdk1p) to the inactivation of APCCdh1. First, in cdc4-arrested cells, which block in late G1 with high levels of G1 CDK activity, ectopically expressed M phase B-type cyclin Clb2p was stable, and its stability required the expression of G1 cyclins (Amon et al., 1994; Amon, 1997). Second, the accumulation of a constitutively expressed Clb2p correlated with the rise in G1 cyclin expression that occurs in G1 (Amon et al., 1994). Finally, Cdh1p was heavily phosphorylated and presumed to be inactive in late G1-arrested cells (Zachariae et al., 1998). Together, these experiments suggested that G1 CDK phosphorylation of Cdh1p was the mechanism for inactivating APCCdh1. However, these experiments were potentially complicated by the fact that Clb2p is not only an APC/C substrate but also an APC/C regulator whose expression might influence its own stability (Amon, 1997).
Unexpectedly, we found that another APCCdh1 substrate, the microtubule-binding protein Ase1p, was rapidly degraded in late G1 (cdc4 block). Further, Ase1p degradation at this arrest point had the hallmarks of APCCdh1-dependent proteolysis: it required both the Ase1p destruction box and Cdh1p. A minimal Ase1p sequence for APCCdh1-mediated degradation was defined whose constitutive expression did not affect cell cycle progression. Using this degradation signal to monitor APCCdh1 activity in vivo, we found that APCCdh1 is inactivated during S phase and that the S phase cyclin Clb5p was required for the normal timing of APCCdh1 inactivation. Further, we show that the degradation of Cdc20p in late G1 is also APCCdh1 dependent and that premature expression of Cdc20p delays the progression through S phase. These findings suggest that both G1 and S phase cyclins are required to shut off APCCdh1 and that APCCdh1 activity in G1 plays an important role in ensuring the proper timing of Cdc20p expression.
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Results |
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To determine if Ase1p destruction was inactivated by G1 CDK activity, the half-life of Ase1p was determined in a cdc4 mutant, which arrests before S phase with high G1 CDK activity and high levels of Sic1p. We found that Ase1p is rapidly degraded at the cdc4 block with similar kinetics to that observed previously in -factorarrested cells (Fig. 1
A; Juang et al., 1997). The 510 min half-life of Ase1p at the cdc4 arrest point contrasts sharply with the >60 min half-life of Ase1p observed in cycling or nocodazole-arrested cells (Juang et al., 1997; data not shown). Ase1p is also rapidly degraded in a skp1-11 strain, which also arrests in late G1 (data not shown). This also contrasts with the degradation of the APCCdh1 substrate Clb2p, which is stable at the cdc4 block (Amon et al., 1994; Amon, 1997; unpublished data; see Fig. 4
B, glu). Like Ase1p degradation in
-factorarrested cells, Ase1p degradation at the cdc4 block required Cdh1p and the destruction box (db), a cis-acting sequence for APC/C-mediated degradation (Fig. 1, A and B). Therefore, Ase1p is rapidly degraded by APCCdh1 in the presence of high levels of G1 CDK activity.
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Many of the known APC/C substrates are also APC/C regulators (Amon, 1997; Charles et al., 1998; Shirayama et al., 1998). This complicates the in vivo analysis of the proteolysis of these proteins because of the possibility that ectopic expression of these proteins during half-life measurements could itself alter APC/C function (Amon, 1997).
Ase1p is a microtubule-binding and cross-linking protein that is required for the structural integrity of the anaphase spindle (Juang et al., 1997; unpublished data). Although Ase1p is not expected to regulate APC/C, high levels of Ase1p activate the spindle assembly checkpoint and thereby might indirectly affect APC/C activity (Juang et al., 1997; for review see Amon, 1999). To create an inert reporter of APCCdh1 activity, we constructed chimeras between Ase1p and glutathione S-transferase (GST). These chimeras enabled us to define the minimal Ase1p sequence necessary for APCCdh1-mediated degradation. The half-lives of the GSTAse1p fusions were measured in -factorarrested cells (Fig. 2
A). A chimera containing the COOH-terminal 254 amino acids (R632-I885) of Ase1p was degraded with similar kinetics to full-length Ase1p. Further deletions up to amino acid 802 from the COOH-terminal end of R632-I885 were also rapidly degraded in
-factorarrested cells. By contrast, deletion of 22 amino acids on the NH2-terminal side of the R632-I885 peptide resulted in a stable chimera (T654-I885; Fig. 2 A). 7 of these 22 residues are lysines, raising the possibility that these may be the site(s) of Ase1p ubiquitination. Together, these results suggest that residues 632802 comprise the minimal sequence for Ase1p degradation in
-factorarrested cells.
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The localization of full-length Ase1p is restricted to the spindle midzone (the zone of overlap between the two half-spindles) (Pellman et al., 1995). By contrast, the C254 fusion protein localized diffusely throughout the nucleus and as expected did not complement an ase1 mutation (Fig. 2 E; data not shown). Therefore, the specific localization of Ase1p on the mitotic spindle is not required for the normal timing of its degradation.
The finding that Ase1p was degraded by APCCdh1 at the cdc4 block suggested that G1 CDK activity was not sufficient to fully inactivate APCCdh1. To monitor APCCdh1 activity during a normal cell cycle, C254 was expressed from the GALL promoter (Mumberg et al., 1994), a derivative of the GAL1,10 promoter that is transcribed at low levels throughout the cell cycle when cells are grown in galactose-containing medium. Importantly, constitutive expression of C254 did not affect cell growth (data not shown) or cell cycle progression (Fig. 3 A). Because transcription of C254 from this promoter was constant during the cell cycle (Fig. 3 B), the steady-state level of C254 protein reflected its degradation.
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Because APCCdh1 appeared to be inactivated during S phase, we determined if removal of Clb5p, the major S phase cyclin (Epstein and Cross, 1992; Schwob and Nasmyth, 1993), would affect the accumulation of C254. Consistent with a role for Clb5p in APCCdh1 inactivation, C254 accumulation was delayed by 10 min in a clb5
strain (Fig. 3 C). By contrast, deletion of CLB3 and CLB4, which encode M phase cyclins, had no effect on the timing of C254 destruction (data not shown). Therefore, the normal timing of APCCdh1 inactivation requires Clb5p.
We considered the possibility that ectopic expression of Clb2p could overcome the high levels of Sic1p in late G1-arrested cells and combine with G1 CDK activity to inactivate APCCdh1. This could explain the difference between the timing of degradation of Ase1p and Clb2p. To test this idea, cdc4 cells constitutively expressing low levels of Clb2p were synchronized with -factor and released at 36°C to the cdc4 arrest point in the presence or absence of hemagglutinin (HA)-tagged Sic1p overexpressed from the GAL1,10 promoter (Verma et al., 1997). As expected, Clb2p accumulated in the control culture (Fig. 4 A, glu). However, Clb2p accumulation was prevented in the Sic1p-overexpressing culture (Fig. 4, compare
F with gal). Northern blot analysis showed that CLB2 mRNA levels did not differ between the two cultures (Fig. 4 A). We next measured the half-life of Clb2p in cdc4-arrested cells in the presence or absence of Sic1p overexpression. In the absence of Sic1p overexpression, Clb2p has a half-life of >60 min, whereas with cooverexpression of Sic1p, Clb2p half-life is
20 min. FACS® analysis showed that the cells remained arrested in G1 for
30 min after the samples were collected (Fig. 4 B). Together, our results suggest that a combination of G1 cyclins and either S phase or M phase cyclins are required to inactivate APCCdh1.
The persistence of APCCdh1 activity through late G1 suggested that the degradation of certain APCCdh1 substrates might be required for the normal execution of S phase. One appealing candidate for such a substrate is the APC activator Cdc20p. Metazoan Cdc20p is an APCCdh1 substrate (Pfleger and Kirschner, 2000; Sorensen et al., 2000), and premature activation of APCCdc20 might interfere with the expression of S phase cyclins. Indeed, we found that degradation of yeast Cdc20p is APCCdh1 dependent in cdc4-arrested cells (Fig. 5 A).
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Discussion |
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Our conclusions differ from that of previous work using Clb2p degradation to monitor APCCdh1 activity in vivo. The prior work suggested that G1 CDKs alone could inactivate what is now known to be APCCdh1 (Amon et al., 1994). The difference in the timing of Clb2p, Ase1p and Cdc20p degradation reported here could either be due to intrinsic differences in how these proteins are recognized by APCCdh1 or to effects from the ectopic expression of Clb2p. Although we found that some Cdh1p is bound to APC/C in cdc4-arrested cells, considerably less is bound than in -factorarrested cells. It is therefore possible that APCCdh1 is a more efficient enzyme for Ase1p and Cdc20p ubiquitination than for Clb2p ubiquitination. However, we also found that cooverexpression of Sic1p at the cdc4 arrest point blocks Clb2p accumulation and restores Clb2p degradation in cdc4-arrested cells. This suggests that ectopic Clb/CDK activity was responsible for turning off the APCCdh1 in the previous experiments. We therefore favor the idea that both G1- and B-type cyclinassociated kinases are required to fully inactivate APCCdh1. Clb2p expressed in late G1 could either directly inactivate the APCCdh1 or compete for Sic1p binding to S phase cyclins thereby freeing them to inhibit APCCdh1.
How might G1 and S phase CDKs cooperate to inactivate APCCdh1? Despite the presence of highly phosphorylated Cdh1p at the cdc4 block, enough APCCdh1 remains for rapid degradation of Ase1p and Cdc20p. Since Cdh1p has multiple functionally important CDK phosphorylation sites (Zachariae et al., 1998; Jaspersen et al., 1999), G1 and S phase CDK might preferentially phosphorylate different sites or make an additive contribution to the total level of Cdh1p phosphorylation. Alternatively, the inhibitory effects of S phase CDK activity could be through phosphorylation of another substrate such as subunits of APC (Rudner and Murray, 2000). Whatever the mechanism for Clb5p-mediated inactivation of APCCdh1, these results identify a novel Clb5p role in the inactivation of APCCdh1 and by inference in the accumulation of mitotic cyclins.
These experiments also have implications for the relationship among Clb5p, APCCdh1, and APCCdc20. In both the Xenopus extract system and in cells overexpressing human Cdh1p, APCCdh1 promotes the degradation of human Cdc20p (Pfleger and Kirschner, 2000; Sorensen et al., 2000). Our finding that yeast Cdc20p is also degraded by APCCdh1 in late G1 demonstrates that this regulatory mechanism is conserved. Further, our in vivo experiments suggest that inappropriate expression of Cdc20p can delay progression through S phase. Therefore, we propose that the following regulation occurs at the G1/S transition. APCCdh1 is active throughout G1. Clb5p accumulates in late G1, but Clb5/CDK activity is held in check by the presence of Sic1p (Schwob et al., 1994). After Sic1p is degraded, Clb5/CDK activity drives DNA replication and inactivates APCCdh1. Because Cdc20p is an APCCdh1 substrate, APCCdh1 activity ensures that no Cdc20p is present to activate APCCdc20 until after Clb5/CDK is active. It should be emphasized that degradation of Cdc20p by APCCdh1 is one of several controls on Cdc20p expression as CDC20 transcription is also low in G1 (Prinz et al., 1998; Shirayama et al., 1998). Additionally, full APCCdc20 activation also appears to require the phosphorylation of the APC/C itself (Kotani et al., 1999; Shteinberg et al., 1999; Kramer et al., 2000; Rudner and Murray, 2000). The existence of multiple overlapping control mechanisms is a common theme in the regulation of cell cycle transitions. We make the analogy to the regulation of mitotic exit where proteolysis, decreased transcription, and the expression of an inhibitor all collaborate to shut off mitotic cyclins.
Finally, our findings impact on the physiological role of Clb5p degradation by APCCdc20. Mutant cells lacking Cdc20p but able to separate sister chromatids (cdc20 pds1
strains) cannot exit from mitosis (Shirayama et al., 1999). However, these cells are able to exit mitosis if CLB5 is deleted. Based on these findings, it was suggested that Cdc20p promotes mitotic exit in a normal cell cycle by degrading Clb5p, which in turn releases inhibition of Sic1p and Cdh1p. The results presented here raise the possibility that the role of Clb5p degradation in mitotic exit is indirect and occurs by suppression of mitotic cyclins. In the absence of Clb5p, APCCdh1 may remain partially active, resulting in decreased levels of the mitotic cyclins Clb1p and Clb2p. In cells with reduced levels of mitotic cyclins, APCCdh1 alone may be sufficient to trigger mitotic exit. The latter model is consistent with the established role of mitotic cyclin proteolysis in mitotic exit.
The APC/C is strikingly conserved among eukaryotes. The conservation of the APC/C is seen not only in the peptide sequence of its components and substrates but also extends to aspects of its regulation. Our results highlight what may be a general role for S phase cyclins in regulating APCCdh1. In human cells, the S phase cyclin A complexed to Cdk1p phosphorylates and inactivates Cdh1p during S phase (Lukas et al., 1999). Thus, S phase inactivation of APCCdh1 appears to be a conserved mechanism for control of the cell cycle.
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Materials and methods |
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Mutagenesis and cloning
DNA manipulations were performed as described (Sambrook et al., 1989). Ase1p truncations were generated by PCRs. The sequence of the oligonucleotide primers used for PCR are available upon request. All PCR-generated constructs were verified by DNA sequencing.
Half-life determination
Cells were arrested in medium containing 3% raffinose; galactose was added to a final concentration of 3% for 3060 min, and then glucose (final concentration 3%) and cycloheximide (final concentration 1 mg/ml) were added to shut off transcription and translation. For proteins expressed from the MET25 promoter, methionine (final concentration 1 mM) and cycloheximide were added. Protein samples were prepared from cells collected at the indicated time points and analyzed by Western blotting. Protein extracts were made as described (Yaffe and Schatz, 1984). Immunoblots were developed using ECL. Ase1p and derivatives were detected with either 9E10 (Evan et al., 1985) for myc-tagged proteins or a rabbit polyclonal anti-Ase1p antibody (Juang et al., 1997). myc-tagged Cdc16p was detected with 9E10. HA-tagged Clb2p, Cdh1p, and Cdc20p were detected with the 12CA5 monoclonal antibody (Field et al., 1988). Polyclonal antibodies were used to detect -tubulin (Serotec), ß-tubulin (a gift from F. Solomon, Massachusetts Institute of Technology, Cambridge, MA), and Sic1p (a gift from J.W. Harper, Baylor College of Medicine, Houston, TX). For quantification in Fig. 4, immunoblots were developed with ECL+, fluorescence was detected using StormImager (Molecular Dynamics), and signal intensities were measure using ImageQuant 5.0 software. For quantification in Fig. 5, immunoblots were probed with an IRDye800 fluorescent-labeled secondary antibody and quantitated on the Odyssey Infrared Imaging System (Li-Cor); fluorescence between blots was normalized to reference standards that were loaded on both blots.
Immunoprecipitation
Strains containing myc-tagged Cdc16p (or untagged control) and HA-tagged Cdh1p were arrested at either 36°C (-factor, cdc4) or 24°C (nocodazole). Cells were lysed using glass beads in IP buffer (50 mM Hepes, pH 7.3, 150 mM potassium acetate, 10 mM sodium fluoride, 1 mM EDTA, 1 mM PMSF, 20 mM ß-glycerophosphate, 1 mM sodium vanadate, Phosphatase Inhibitor sets I and II [Calbiochem], 0.1% Triton X-100, and CompleteTM protease inhibitors [Roche]). Extracts (24 mg) were incubated with 9E10 monoclonal antibodies (1,000:1) in IP buffer for 2 h at 4°C. Antibody-bound extracts were then incubated with protein Gplus agarose beads (Calbiochem) for 4 h at 4°C in IP buffer. The beads were washed five times with IP buffer and resuspended in sample buffer. Bound proteins were analyzed by SDS-PAGE and immunoblotting.
Flow cytometry
Cells were prepared for FACS® analysis as described (Pellman et al., 1995) and analyzed on a FACScanTM using CellQuest software (Becton Dickinson).
Northern blotting
RNA preparation, Northern blot analysis, and the probes to detect histone 2B1 and actin mRNA were as described (Pellman et al., 1995). The probe for ASE1 mRNA was a 1.2-kb fragment from nucleotides 18963108 of the ASE1 locus (where 1 is the A residue of the ATG codon).
Immunofluorescence
Cells were fixed and prepared for DAPI staining and immunofluorescence as described previously (Pringle et al., 1991). C254 containing three COOH-terminal myc tags was detected with 9E10.
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Footnotes |
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Acknowledgments |
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This work was supported by an Howard Hughes Medical Institute Postdoctoral Research Fellowship for Physicians (to J.N. Huang), a Kimmel Scholar Award (to D. Pellman), and National Institutes of Health grants (K08 HD01203 and P30 HD27823-10 to J.N. Huang and RO1 GM55772 to D. Pellman).
Submitted: 1 February 2001
Revised: 11 May 2001
Accepted: 25 May 2001
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