Article |
Address correspondence to Michael B. Yaffe, Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Ave., E18-580 Cambridge, MA 02139. Tel.: (617) 452-2442. Fax: (617) 452-4978. E-mail: myaffe{at}mit.edu
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Abstract |
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Key Words: 14-3-3; FKHRL1; phosphoserine; nuclear export; Crm1
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Introduction |
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Insight into the mechanism of 14-3-3dependent cytosolic sequestration has emerged from a series of studies on the interaction between 14-3-3 and Cdc25. Initial studies by Lopez-Girona et al. (1999) identified a leucine-rich sequence within the COOH-terminal helix (helix I) of 14-3-3 that was highly homologous to known nuclear export signals (NESs).* These authors proposed that 14-3-3 functioned essentially as an "attachable NES" to transport nuclear Cdc25 to the cytoplasmic compartment by a piggy-back mechanism. This view was contradicted by detailed studies by both Kumagai and Dunphy (1999) and Zeng and Piwnica-Worms (1999). These authors showed that point mutations in this leucine-rich sequence of 14-3-3 prevented Cdc25 from being sequestered in the cytoplasm by disrupting its binding to 14-3-3. Furthermore, our x-ray crystallographic studies of 14-3-3peptide complexes of two canonical 14-3-3 binding motifs R[S/Ar]XpSXP and RX[Ar/S]XpSXP, (pS denotes phosphoserine and Ar denotes aromatic residues) showed that the critical leucine and isoleucine residues within helix
I were directly involved in binding to the phosphopeptide (Rittinger et al., 1999). Taken together, these studies raise the possibility that the primary function of helix
I may be to mediate interactions between 14-3-3 and many phosphoprotein ligands but do not prove that this sequence cannot function as an NES.
The current prevailing mechanism for how 14-3-3 proteins mediate changes in the subcellular localization of Cdc25 comes from elegant studies by Kumagai and Dunphy (1999) and Yang et al. (1999). Cdc25 was found to be exported from the nucleus at a constant rate. This process involved intrinsic NES sequences in Cdc25 but was not modified by phosphorylation/14-3-3 binding. In contrast, 14-3-3bound Cdc25 within the cytoplasm showed a decreased rate of nuclear import as a result of masking a Cdc25 nuclear localization signal (NLS) sequence, thereby inhibiting the interaction of Cdc25 with importin-. Whether this mechanism operates for all 14-3-3bound ligands or whether a subset of ligands can undergo an increase in nuclear export upon phosphorylation/14-3-3 binding is not known.
In the present study, we sought to clarify where 14-3-3 binding to its ligands occurred, determine whether the leucine-rich helix I truly functions as an NES, and identify features of both 14-3-3 and one of its nuclear ligands, the transcription factor FKHRL1, that are critical for the movement of this nuclear ligand to the cytoplasm. Surprisingly, we find that in the absence of bound ligands the majority of 14-3-3 is localized to the nucleus, where a large number of 14-3-3 ligands are present. For FKHRL1, growth-factor stimulation, which activates AKT, first leads to the phosphorylation of FKHRL1 at its 14-3-3 binding site within the nucleus immediately before FKHRL1 nuclear export. Helix
I of 14-3-3 does not function as an attachable NES but is involved in a general manner in the binding of phosphorylated ligands. Instead, rapid nuclear export of FKHRL1 requires both NES sequences that are intrinsic to FKHRL1 and phosphorylation/14-3-3 binding. Once in the cytoplasm, FKHRL1 remains phosphorylated and bound to 14-3-3, thereby preventing nuclear reimport. Together with the studies on Cdc25 cited above, our study suggests that 14-3-3 can function through multiple mechanisms that may cooperate to ensure complete sequestration of the phosphorylated 14-3-3 ligand in the cytoplasm.
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Results |
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To determine if helix I of 14-3-3 has a very general function in binding to many serine-phosphorylated 14-3-3 binding proteins, we used commercial antibodies that had been generated against one of the major 14-3-3 phosphoserine binding motifs. This motif is present in many 14-3-3 ligands (Muslin et al., 1996; Yaffe et al., 1997), and this antibody detects a large number of immunoreactive bands in 293T total cell lysates (Fig. 3 A, TCL lane), which are specifically pulled down by wild-type (WT) 14-3-3GST (14-3-3
WT lane) but not by a 14-3-3GST point mutant that is unable to bind to ligands (14-3-3
MT lane, discussed in more detail below). Deletion of residues 215255 in 14-3-3
, which includes both helix
I and the extreme COOH-terminal tail (Fig. 3 A,
C lanes, and Fig. 4 A), completely abrogated binding of 14-3-3 to all of the detected proteins. The extreme COOH-terminal tail of 14-3-3 (residues 233255 in
) cannot be visualized in 14-3-3 x-ray structures (Liu et al., 1995; Xiao et al., 1995) (Fig. 4 A), and its contribution to ligand binding is unknown. Removal of this region while maintaining helix
I reduced but did not eliminate 14-3-3 binding (Fig. 3 A,
T lanes), demonstrating that helix
I plays an essential role in binding to many phosphorylated protein ligands.
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A 14-3-3 mutant, which cannot bind to ligands, accumulates in the nucleus
The experiments described above strongly argue that helix I functions primarily in ligand binding but cannot completely exclude the possibility that helix
I might also somehow mediate nuclear export. To directly address whether helix
I can function as an NES in vivo, we took advantage of a 14-3-3 point mutant in which Lys-49 is replaced by Glu (K49E) in the presence of an intact WT helix
I. This mutant, developed by Fu and colleagues, has the same conformation as WT 14-3-3 and is able to form homo- and heterodimers in vitro and in vivo (Zhang et al., 1997; unpublished data) but is unable to bind to Raf (Zhang et al., 1997). X-ray crystallographic studies revealed that Lys-49 forms part of a positively charged substrate-binding pocket in the central binding groove of 14-3-3 (Fig. 4 A), where it directly coordinates the phosphoserine phosphate of 14-3-3 binding peptides (Yaffe et al., 1997; Petosa et al., 1998; Rittinger et al., 1999). These structural observations on peptides suggested that the K49E mutation would be generally deficient in binding to phosphorylated protein ligands, while preserving its leucine-rich NES-like sequence. Indeed, the 14-3-3
K49E mutant is unable to bind to numerous 14-3-3 binding proteins in cell extracts (Fig. 3 A, MT lanes), allowing the intrinsic function of the leucine-rich sequence in helix
I to be directly investigated without complexities arising from 14-3-3 ligand interactions.
If the leucine-rich sequence in helix I functions as a true NES for 14-3-3, then the unliganded 14-3-3
K49E protein should localize exclusively in the cytoplasm. Stable U2OS cell lines expressing either WT or K49E mutant Xpress-tagged 14-3-3
proteins were generated, and three different stable cell lines for each construct were studied in detail (Fig. 4, BD). The ligand-binding defective 14-3-3
K49E mutant was found predominantly in the nucleus in 100% of the stable transfected cells in all cell lines investigated (Fig. 4 D, left). In contrast, the tagged WT 14-3-3 protein was found almost exclusively in the cytoplasm (Fig. 4 D, right), showing the identical staining pattern as that seen with endogenous 14-3-3 (Fig. 1 A). Expression levels of tagged WT 14-3-3
in all three cell lines were
3.5- to 5-fold lower than those seen with the K49E mutant (Fig. 4, B and C), perhaps due to deleterious effects of 14-3-3 on cell growth (Hermeking et al., 1997; Kumagai et al., 1998). However, in all cases the expression levels of the stably transfected 14-3-3 proteins were less than or equal to those of endogenous 14-3-3 (Fig. 4 C). The possibility that localization of the K49E mutant compared with WT was due to differences in expression levels was ruled out by transient transfection experiments. Under these conditions, both WT and mutant proteins are expressed at equivalent levels (albeit at significantly higher concentrations than the endogenous 14-3-3 proteins) and resulted in the same nuclear localization for the K49E mutant (unpublished data). Together, these findings show conclusively that the leucine-rich sequence within 14-3-3 does not function as an NES in the absence of bound ligands.
The nuclear localization of the K49E 14-3-3 mutant strongly argues that nuclear export of 14-3-3 requires ligand binding. In addition, the LMB experiments indicate that the 14-3-3 is exported in an NES-dependent manner. Therefore, we hypothesized that it is NES sequences on the bound ligand which drive the export of 14-3-3ligand complexes out of the nucleus and that this process is facilitated by 14-3-3 binding. To directly investigate the contribution of the 14-3-3bound cargo to nuclear export, we focused the remainder of this study on one particular 14-3-3 binding ligand, the human Forkhead transcription factor FKHRL1 (Brunet et al., 1999).
The export of the 14-3-3 ligand FKHRL1 requires Crm1, intrinsic FKHLR1 NES sequences, and 14-3-3 binding
The subcellular localization of FKHRL1 is dependent on its phosphorylation by the protein kinase Akt. In the absence of growth factor, Akt is inactive and FKHRL1 is present in the nucleus in an unphosphorylated form. Upon growth factor stimulation, Akt is activated, directly phosphorylating FKHRL1 at T32 and S253, and to a lesser extent at S315, and resulting in FKHRL1 relocalization from the nucleus to the cytoplasm (Brunet et al., 1999). Similar AKT-dependent changes in subcellular localization have also been observed for the additional FKHRL1 human homologs AFX and FKHR (Brownawell et al., 2001; Rena et al., 2001) and for the Caenorhabditis elegans homologue daf-16 (Cahill et al., 2001). Phosphorylation at T32 and S253 causes FKHRL1 to bind to 14-3-3, suggesting that the interaction between FKHRL1 and 14-3-3 may play an important role in regulating the subcellular localization of the complex. COOH-terminal to the two 14-3-3 binding sites, FKHRL1 contains two sequences that closely match a consensus for NES sequences (Fig. 5 A), suggesting that nuclear export of 14-3-3FKHRL1 complexes might involve a cooperative process involving both the FKHRL1 NES and 14-3-3 binding.
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To investigate whether the intrinsic FKHRL1 NES sequences were responsible for the NES-dependent FKHRL1 nuclear export, we generated mutants of FKHRL1 in which the critical leucine or methionine residues in NES1 or NES2 were replaced by alanines either individually or in combination (Fig. 5, C and D). We found that an FKHRL1 mutant in which both NES1 and -2 were mutated remains localized in the nucleus even in the presence of growth factors, indicating that the NES sequences of FKHRL1 are required for the nuclear export of this protein. Importantly, we verified that the FKHRL1 NES mutant protein, which localizes to the nucleus, still binds to 14-3-3 (Fig. 5 E). This indicates that in the absence of a functional NES in FKHRL1, 14-3-3 binding alone is not sufficient to promote the efficient localization of FKHRL1 from the nucleus to the cytoplasm.
Consistent with our previous results, we found that a triple point mutant of FKHRL1, which does not bind to 14-3-3 (Fig. 5 E) because the key 14-3-3 binding residues T32 and S253 has been mutated to alanine, remains localized within the nucleus even in the presence of growth factors (Fig. 5, C and D). As this FKHRL1 mutant contains the intact NESs, this result further indicates that the NESs of FKHRL1 are not sufficient to lead to the net export of FKHRL1 out of the nucleus in the absence of 14-3-3 binding. Therefore, both the NES of FKHRL1 and FKHRL1's ability to bind 14-3-3 appear to be necessary to promote efficient FKHRL1 nuclear export. In addition, we found in transient transfection studies that FKHRL1 bound to Crm1 in a manner that is dependent on FKHRL1 NESs, further confirming that the NES sequences of FKHRL1 play a role in a Crm1-dependent nuclear export of this transcription factor (unpublished data). However, in these overexpression studies we have been unable to show that the interaction between FKHRL1 and Crm1 is facilitated by 14-3-3. Nevertheless, 14-3-3 may enhance the binding of FKHRL1 to Crm1 in a context where both proteins are expressed at physiological levels, or alternatively 14-3-3 may enhance the export of FKHRL1 without directly enhancing its binding to Crm1.
FKHLR1 undergoes phosphorylation within the nucleus upon growth factor stimulation before nuclear exportroles of intrinsic NES and NLS sequences and the 14-3-3 binding site in FKHRL1 localization
The experiments described above reflect the overall subcellular distribution of FKHRL1 at a given time but do not address the dynamic sequence of events involving FKHRL1 relocalization from the nucleus to the cytoplasm. Based on the results from Figs. 1 4, we hypothesized that 14-3-3 binding to FKHRL1 might occur in the nucleus, exposing the FKHRL1 NES and facilitating export of the 14-3-3FKHRL1 complex to the cytoplasm. Once in the cytoplasm, 14-3-3 may have additional function in masking potential NLS, thereby preventing reentry of the ligands into the nucleus.
This sequence of events would require the phosphorylation of FKHRL1 in the nucleus to precede its cytoplasmic translocation. To investigate this, WT HA-tagged FKHRL1 was expressed in CCL39 fibroblasts, and the kinetics of phosphorylation and FKHRL1 relocalization were examined using immunofluorescence experiments with an antibody to HA and an antibody specific to phospho-Thr32 (Fig. 6, WT panels). When cells were deprived of survival factors, WT FKHRL1 was localized in the nucleus and was not phosphorylated at Thr32. At short times after stimulation by IGF-1 phosphorylation of WT FKHRL1 at the 14-3-3 consensus binding site, Thr32 was detected, whereas FKHRL1 remained in the nucleus (Fig. 6, WT, 2 min). Upon longer stimulation times, the phosphorylated form of FKHRL1 began to appear predominantly in the cytoplasm (Fig. 6, WT, 5 and 15 min). In contrast, the NES1/2 mutant (NESm) was efficiently phosphorylated by IGF-1 within the nucleus, but unlike WT FKHRL1 the FKHRL1 NESm remained within the nucleus even after relatively long incubation times (Fig. 6, NESm 2, 5, and 15 min panels). Thus, phosphorylation of FKHRL1 at Thr32, which generates a 14-3-3 binding site, occurs within the nucleus before FKHRL1 cytoplasmic relocalization. The finding that phosphorylation of FKHRL1 to generate 14-3-3 binding sites occurs within the nucleus together with the nuclear localization of ligand-free 14-3-3 proteins further suggests that 14-3-3 binding to nuclear FKHRL1 may facilitate its NES-dependent export.
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Together, these findings suggest that 14-3-3 binding to ligands can occur within the nucleus and may facilitate the nuclear export of the 14-3-3ligand complex through the NES of the bound ligand. In the cytoplasm, 14-3-3 may have an additional role in preventing the reimport of ligands such as FKHRL1.
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Discussion |
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Our observation that many potential 14-3-3 binding substrates are present in the nuclear fraction and that FKHRL1 is phosphorylated at its major 14-3-3 binding site within the nucleus suggests that kinases, such as AKT, Chk1, and Chk2, may act preferentially within this compartment, conferring 14-3-3 binding to some of their substrates. Furthermore, the observations that LMB treatment led to accumulation of endogenous 14-3-3 in the nucleus and that a K49E mutant of 14-3-3, which does not bind to ligands, localizes preferentially within the nucleus suggests that unliganded 14-3-3 normally transits to the nuclear compartment where binding to some ligands can occur. It would also be interesting to investigate the rate of import of 14-3-3 into the nucleus to address whether dissociation of ligands from preformed cytoplasmic complexes determines the rate of 14-3-3 nuclear import. We found that elimination or mutation of the leucine-rich sequence within helix I disrupts binding to numerous phosphorylated protein ligands. This is in excellent agreement with the results of more focused mutational studies reported by others examining 14-3-3 binding to Raf and Cdc25 (Thorson et al., 1998; Wang et al., 1998; Kumagai and Dunphy, 1999; Zeng and Piwnica-Worms, 1999). Thus, the primary and general function of this
-helix is to directly participate in ligand binding. Furthermore, the observation that this leucine-rich sequence is incapable of maintaining unliganded 14-3-3 in the cytoplasm is incompatible with its proposed function as a true NES (Lopez-Girona et al., 1999). Instead, our present study of FKHRL1 and previous studies on Cdc25 (Kumagai and Dunphy, 1999; Yang et al., 1999; Davezac et al., 2000) indicate that export of the 14-3-3bound complex involves NES sequences that are intrinsic to the ligands.
We reported previously an interaction between 14-3-3 and Crm1 within eukaryotic cell lysates that was disrupted by a 14-3-3 binding peptide (Rittinger et al., 1999). At that time, we interpreted this as evidence that the leucine-rich region within helix I could interact with either ligands or Crm1 but not both simultaneously. In light of our current findings, the interaction that we observed between 14-3-3 and Crm1 was most likely mediated through the 14-3-3bound ligands, which were competed off by the 14-3-3 binding peptide. In agreement with this, we have been unable to show a direct interaction between 14-3-3 and Crm1 when both are expressed in Escherichia coli (unpublished data).
Our findings are consistent with several different functions for AKT and 14-3-3 in regulating the activity and subcellular localization of FKHRL1. We have shown that AKT-mediated phosphorylation of FKHRL1 at the 14-3-3 binding site within the nucleus results in accelerated nuclear export of FKHRL1. This role for phosphorylation/14-3-3 binding in nuclear export is novel and complements recent results from other investigators who reported, while our paper was under revision, that AKT phosphorylation of the FKHRL1 homologs AFX and FKHR can inhibit nuclear reimport (Brownawell et al., 2001; Rena et al., 2001). In addition, Brownawell et al. (2001) proposed that phosphorylation at the 14-3-3 binding site did not affect nuclear export, since a triple phosphorylation site mutant of AFX that could not bind to 14-3-3 was able to shuttle between nuclei in heterokaryon fusion experiments. However, this study did not examine the time dependence of the import or export process, and our data in Fig. 8 indicates that phosphorylation at the 14-3-3 binding site is critical for rapid nuclear export of FKHRL1. Therefore, it is likely that AKT phosphorylation of Forkhead family members affects both nuclear export and import: phosphorylation/14-3-3 binding increases the rapid export of FKHRL1 from the nucleus in a manner that requires the intrinsic NES sequences on FKHRL1, whereas phosphorylation near the NLS site may disrupt FKHRL1 subsequent nuclear reimport. Furthermore, Cahill et al. (2001) showed that phosphorylation of the C. elegans FKHRL1 homologue daf-16 by AKT together with 14-3-3 binding directly inhibited its DNA binding ability. Thus, AKT- and 14-3-3mediated inhibition of FKHRL1 function is likely to involve multiple events, first by blocking DNA binding and then by ensuring a complete sequestration of this transcription factor in the cytoplasm away from their nuclear target genes.
We find that a K49E mutant of 14-3-3 is unable to bind to ligands or be exported from the nucleus, despite its ability to form heterodimers with endogenous WT 14-3-3 in vivo and in vitro. This finding underscores the important role of ligand binding by each monomer within the 14-3-3 dimer for 14-3-3 function. A requirement for dimeric 14-3-3 has also been demonstrated for both 14-3-3mediated Raf activation (Tzivion et al., 1998) and for 14-3-3mediated disruption of daf-16 DNA binding after AKT phosphorylation (Cahill et al., 2001) using a mutant 14-3-3 in which the dimerization interface was disrupted.
Our functional data shown in Fig. 8, examining FKHRL1 export as a function of time, shows an increase in nuclear export upon FKHRL1 phosphorylation and 14-3-3 binding, which suggests that 14-3-3 may enhance the export process. Exactly how 14-3-3 accomplishes this remains to be defined. 14-3-3 binding might facilitate the formation of productive complexes between FKHRL1 and regulatory components of the nuclear export machinery, perhaps through inducing conformational changes in the 14-3-3bound ligand. Recent data from Brownawell et al. (2001) on AFX and our unpublished observations on FKHRL1 suggest, however, that 14-3-3 binding does not cause an increase in the bulk association of these transcription factors with Crm1, though both proteins are grossly overexpressed in these assays. It has not been technically possible to measure the kinetics with which the association occurs between the endogenous proteins within cells. Alternatively, 14-3-3 binding could facilitate the ability of FKHRL1Crm1 complexes to bind to endogenous Ran-GTP to trigger nuclear export. Another possibility is that 14-3-3 binding protects phosphorylation sites from the action of phosphatases and/or thereby permits additional modifications of FKHRL1, which are themselves involved in the export process.
We have focused this study specifically on one particular 14-3-3 ligand, FKHRL1, but studies on other 14-3-3 binding ligands such as HDAC4, -5, and -7 and Cdc25 are consistent with the idea that cytoplasmic relocalization involves both 14-3-3 proteins and ligand NES and NLS sequences (Kumagai and Dunphy, 1999; Yang et al., 1999; Davezac et al., 2000; Grozinger and Schreiber, 2000; Wang et al., 2000; Kao et al., 2001). Furthermore, not all 14-3-3bound ligands are destined for cytoplasmic sequestration. For other molecules, 14-3-3 binding may preferentially sequester them within the nucleus as has been reported for the catalytic subunit of human telomerase (Seimiya et al., 2000) and the HOX11 homeobox transcription factor Tlx-2 (Tang et al., 1998). Thus, it appears that the ligand, rather than 14-3-3, dictates the resulting subcellular location (Muslin and Xing, 2000). In this manner, 14-3-3 functions as a type of "molecular chauffeur" where the destination of the 14-3-3bound complex is determined by instructions contained within the sequence and structure of the bound cargo rather than through any intrinsic properties of 14-3-3. The ultimate fate of such cargo is then determined by 14-3-3mediated alterations in the kinetics of dynamic nuclear-cytoplasmic transport.
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Materials and methods |
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Plasmids
Plasmids encoding GST14-3-3, GST14-3-3
K49E, HA-FKHRL1 WT, and FKHRL1 T32A/S253A/S315A have been described previously (Brunet et al., 1999; Rittinger et al., 1999). GST14-3-3
([1214];
C) and GST14-3-3
([1232];
T) were generated by PCR amplification and cDNA cloning into pGEX4T. cDNAs of 14-3-3
WT and 14-3-3
K49E were subcloned into mammalian expression vector pcDNA3.1/His C (Invitrogen) to express Xpress epitope-tagged 14-3-3. The vector encoding the M2-tagged form of 14-3-3
subcloned in the pcDNAneo3 expression vector was provided by Dr. Haian Fu (Emory University School of Medicine, Atlanta, GA). Sequential point mutations of individual leucine and isoleucine residues to alanines in helix
I of GST14-3-3
were generated using specific oligonucleotides and the Quickchange site-directed mutagenesis kit (Stratagene). A mutant of FKHRL1 NES1 (amino acids 369378) and NES2 (amino acids 386396) was obtained as follows: first, a mutant of FKHRL1 NES2 (LMDDAADNATA) was generated using the following set of primers: forward, 5'-AAAGCTGCAGATAACGCCACGGCCCCGCCATCCCAGCCATCGC-3' and reverse, 5'-AAAGGCCGTGGCGTTATCTGCAGCGTCGTCCATGAGGTTTTCAG-3'. The PCR fragment was subcloned into the Acc I sites of HA-FKHRL1 in the pECE vector. FKHRL1 NES2 mutant was then used as a template to generate FKHRL1 NES1-NES2 mutant (LTDMAGTANANDGATENLMDDAADNATA) using the following set of primers: forward, 5'-AAAGCGAATGCGAATGATGGGGCGACTGAAAACCTCATGGACGAC-3' and reverse, 5'-AAACGCCCCATCATTCGCATTCGCGGTGCCTGCCATATCAGTCAGC-3'. The triple point mutants of FKHRL1 NLS sequences were produced with the Quickchange site-directed mutagenesis kit (Stratagene) using the following primers: RRR
AAA forward, 5'-GGGAAGAGCGGAAAAGCCCCCGCGGCGGCGGCTGTCTCCATGGACAATAGC-3' and RRR
AAA reverse, 5'-GCTATTGTCCATGGAGACAGCCGCCGCCGCGGGGGCTTTTCCGCTCTTCCC-3' and KKK
AAA forward, 5'-GAGCCGTGGCCGCGCAGCCGCGGCGGCGGCAGCCCTGCAGACAGCCCCCG-3' and KKK
AAA reverse, 5'-CGGGGGCTGTCTGCAGGGCTGCCGCCGCCGCGGCTGCGCGGCCACGGCTC-3'.
Cell lines and transfection
The 293T human epithelial kidney cell line, human osteosarcoma U2OS cell line, and Chinese hamster lung fibroblast cell line CCL39 were cultured in DME supplemented with 10% FBS, and NIH3T3 cells were maintained in DME with 10% calf serum. LMB was used at final concentration of 20 ng/ml. To establish stable cell lines expressing 14-3-3 proteins, U2OS cells were transfected with pcDNA3.1/His-14-3-3 or pcDNA3.1/His-14-3-3
(K49E) using Superfect (QIAGEN) and transfected cells selected in media containing 400 µg/ml G418 (GIBCO BRL). After 2 wk, resistant colonies were tested for expression of epitope-tagged 14-3-3 by immunoblotting and used to propagate stable cell lines.
Cellular fractionation, immunoblotting, and 14-3-3 binding assays
U2OS cells were suspended in buffer A (10 mM Hepes, pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 0.15% NP-40, 1 mg/ml each of AEBSF, aprotinin, leupeptin, and pepstatin), swollen for 10 min on ice, and centrifuged at 12,000 g for 30 s. Supernatant was collected as a cytoplasmic extract. The pellet was washed, resuspend in buffer B (20 mM Hepes, pH 7.9, 400 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 0.5% NP-40, 4 µg/ml each of AEBSF, aprotinin, leupeptin, and pepstatin), and rocked for 15 min at 4°C. The supernatant after centrifugation was used as nuclear extract. Equal amounts of proteins from cytosol and nuclear extract were subjected to immunoblotting as described (Kanai et al., 2000). To investigate the distribution of 14-3-3 ligands, cells were metabolically labeled in the presence of [35S]methionine for 8 h and then separated into nuclear or cytoplasmic fractions as above. Samples (240 µg total protein containing equal amounts of radioactivity) were adjusted to the same final buffer composition and pulled down with beads containing 150 µg of GST14-3-3 or GST as described (Rittinger et al., 1999). Proteins binding to the beads were eluted, separated by SDS-PAGE, and analyzed by autoradiography. To analyze the interaction between FKHRL1 and 14-3-3, 293T cells transfected with HA-FKHRL1 constructs and M214-3-3
were lysed and subjected to immunoprecipitation and immunoblotting as described previously (Brunet et al., 1999).
Immunofluorescence microscopy
Immunofluorescent microscopy was done as described (Brunet et al., 1999; Kanai et al., 2000). U2OS cells grown on glass coverslips were fixed with 3% formaldehyde, permeabilized with 0.2% Triton X-100, and stained with an anti14-3-3 antibody (K-19) or an anti14-3-3 monoclonal antibody and Cy3-conjugated secondary antibodies. Cells were counterstained with 1 µg/ml DAPI (Sigma-Aldrich) to visualize the nucleus. The anti-Xpress antibody was used to stain 14-3-3expressing stable cell lines. For immunolocalization studies of FKHRL1, CCL39 fibroblasts on glass coverslips in 12-well dishes were transfected with 4 µg of the indicated plasmids using calcium phosphate. After 24 h, the cells were fixed in 4% formaldehyde/2% sucrose, permeabilized with 0.1% Triton X-100, and stained with anti-HA antibody, anti-phosphoT32 antibody, and Cy3- or Cy2-conjugated antimouse IgG secondary antibody and Cy3-conjugated antirabbit IgG secondary antibody. For quantification experiments, 150300 cells were scored according to whether the protein of interest was higher in the nucleus, evenly distributed between nucleus and cytoplasm, or higher in cytoplasm. Microscopic images were acquired using Nikon Diaphot 300 microscope mounted with a Photometrics SenSys CCD camera and processed using Vaytek Imaging Software.
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Footnotes |
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F. Kani's present address is Dept. of Gastroenterology, University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113-8655, Japan.
* Abbreviations used in this paper: IGF, insulin-like growth factor; LMB, leptomycin B; NES, nuclear export signal; NLS, nuclear localization signal; WT, wild-type.
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Acknowledgments |
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A. Brunet was supported by a Goldenson-Berenberg fellowship, F. Kanai was supported by fellowships from the Cell Science Research Foundation and Sankyo Foundation of Life Science, and J. Stehn was supported by a C.J. Martin Fellowship from the National Health and Medical Research Council of Australia. J.V. Frangioni is a postdoctoral fellow of the Howard Hughes Medical Institute. S.N. Dalal was a fellow of the Leukemia Society of America. This work was funded by National Institutes of Health grant HD24926 and HD18655 to M.E. Greenberg, National Institutes of Health grant GM60594, and a Burroughs-Wellcome Career Development award to M.B. Yaffe.
Submitted: 13 December 2001
Revised: 13 December 2001
Accepted: 17 January 2002
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