Article |
Address correspondence to Mariapia A. Degli-Esposti, Centre for Experimental Immunology, Lions Eye Institute, 2 Verdun Street, Nedlands, Western Australia 6009, Australia. Tel.: 61-8-9381-0808. Fax: 61-8-9381-0700. email: mariapia{at}cyllene.uwa.edu.au
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Abstract |
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Key Words: dendritic cell; cytomegalovirus; apoptosis; Bcl-2; Bax
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Introduction |
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Cytomegalovirus (CMV), a member of the ß herpes virus family, is a prime candidate for possessing anti-apoptotic functions, because its replication cycle is relatively slow and the virus persists lifelong within its host (Mocarski, 1996). Previously, infection of human umbilical-vein endothelial or mouse neuronal cells with human CMV (HCMV) and murine CMV (MCMV), respectively, was found to render infected cells resistant to apoptosis (Kovacs et al., 1996; Kosugi et al., 1998; Wang et al., 2000). Unlike other herpes viruses, CMV does not encode homologues of Bcl-2 or FLIP (Roulston et al., 1999). Anti-apoptotic properties have been ascribed to the M45 gene of MCMV, and the UL36 and UL37 genes of HCMV (Goldmacher et al., 1999; Brune et al., 2001; Skaletskaya et al., 2001). Although the mechanism of action for M45 is unknown, UL36 appears to act by inhibiting caspase-8 activation, and UL37 prevents mitochondrial permeabilization (Goldmacher et al., 1999; Skaletskaya et al., 2001). Despite having ascribed anti-apoptotic activities to several CMV proteins, the precise checkpoints targeted by the virus in apoptotic pathways have not been defined.
Central to apoptotic pathways is the action of caspases, a group of aspartate-specific cysteine proteases whose activation is regulated by the Bcl-2 family of proteins (Cory and Adams, 2002; Shi, 2002). The Bcl-2 family is composed of both pro- and anti-apoptotic proteins that share homology in the Bcl-2 homology (BH) regions (Marsden and Strasser, 2003). The current paradigm suggests that pro-survival Bcl-2 family proteins prevent caspase activation by maintaining mitochondrial integrity (Gross et al., 1999). The activity of pro-survival Bcl-2 proteins is counteracted by two groups of pro-apoptotic proteins, namely, Bax/Bak and the BH3-only group of proteins. BH3-only proteins are regulated by a variety of mechanisms, such as sequestration to the cytoskeleton, which favorably positions these molecules to act as sensors for apoptotic stimuli (Puthalakath and Strasser, 2002). In response to apoptotic stimuli, BH3-only proteins translocate to the mitochondria where it appears they antagonize the function of pro-survival Bcl-2 proteins (Puthalakath and Strasser, 2002).
The BH3-only proteins require the presence of the Bax/Bak proteins for killing. Bax, normally a monomer located in the cytoplasm, undergoes a conformational change, translocates to the mitochondria, and oligomerizes once activated (Hsu et al., 1997; Wolter et al., 1997). Similarly, apoptotic stress results in conformational changes and oligomerization of Bak, although Bak is constitutively localized to mitochondria (Griffiths et al., 1999; Nechushtan et al., 2001). Activation of Bax/Bak at mitochondria is thought to induce permeabilization of the outer mitochondrial membrane, thereby allowing the release of pro-apoptotic proteins, such as cytochrome c and Smac/DIABLO, and the subsequent activation of caspases (Gross et al., 1999). Retention of Bax in the cytoplasm most likely acts as a safe guard against inappropriate activation of the cell death machinery. Consistent with this theory is the observation that enforced dimerization of Bax induces apoptosis, and Bax mutants that are constitutively localized to mitochondria are more effective killers (Gross et al., 1998; Suzuki et al., 2000). However, cells exposed to the apoptosis inducing drug taxol were shown to survive despite the fact that Bax dimerized and translocated to the mitochondria (Makin et al., 2001). Furthermore, detachment of epithelial cells from ECM resulted in reversible changes to Bax conformation and localization (Gilmore et al., 2000; Valentijn et al., 2003). These results suggest that activation of Bax is not sufficient to commit to apoptosis, and that an additional checkpoint may exist.
The ability of CMV to successfully escape immune responses and to establish persistent infection of its hosts led us to hypothesize that this virus would be capable of interfering with apoptosis in cells that are key immune effectors. DCs are specialized antigen presenting cells critical for the initiation and regulation of both innate and adaptive immune responses (Banchereau et al., 2000). Our recent studies have identified DCs as a major target for MCMV infection, both in vivo and in vitro (Andrews et al., 2001). Here, we have demonstrated that MCMV-infected DCs become resistant to apoptosis. Furthermore, we have assessed the precise contribution of changes in the expression of Bcl-2 family proteins and have defined the specific point at which MCMV interferes in the apoptotic pathway. Our studies provide evidence that translocation of Bax to mitochondria, and oligomerization of Bax and Bak are not sufficient for the initiation of apoptotic cell death.
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Results |
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Initially, we determined whether MCMV could prevent apoptosis of D1 cells after growth factor withdrawal. D1 DCs were infected with MCMV at a multiplicity of infection (MOI) >3 and cultured for 4 d, by which time 100% of the cells are MCMV infected (Andrews et al., 2001). MCMV-infected D1, and uninfected control D1 cells, were washed and replated in either normal growth medium or in medium lacking CM. After 16 h the number of viable cells was determined by flow cytometry using annexin V-FITC staining. Consistent with previous reports, a significant decrease in the percentage of viable cells was observed when CM was removed from DC cultures (Fig. 1 a; Rescigno et al., 1998). Conversely, withdrawal of CM from DCs infected with MCMV did not affect cell viability (Fig. 1 a).
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Although activation of caspases is considered a crucial effector of apoptosis, some evidence exists for a caspase-independent apoptotic pathway (Marsden and Strasser, 2003). We examined whether caspases are activated during apoptosis of DCs. Uninfected, LPS-stimulated, and MCMV-infected D1 DCs were grown in the presence or absence of growth factor for 12 h and total cell lysates prepared. Caspase activity in the lysates was measured using a colorimetric substrate (Ac-DEVD-pA) for caspase-3 and related caspases. After growth factor withdrawal from uninfected immature D1 cells, a significant increase in caspase activity was observed (Fig. 1 c). No change in caspase activity was observed after growth factor removal from LPS-stimulated or MCMV-infected cells (Fig. 1 c).
We then tested the effect of MCMV infection on the viability of in vitro expanded DCs. These experiments were performed using DCs generated from the bone marrow by Flt3L expansion (Brasel et al., 2000). This method generates cells with phenotypic and functional characteristics of DCs found in lymphoid tissues (Brasel et al., 2000). After in vitro expansion, DCs were infected with MCMV (MOI >3) and cultured in the presence of Flt3L for a further 4 d. MCMV-infected DCs and uninfected control DCs were then washed and replated in growth medium containing 10% FCS and Flt3L or in medium containing 1% FCS only. The number of viable cells was determined 16 h later by annexin V-FITC staining. Removal of FCS and Flt3L resulted in a significant reduction in the percentage of viable cells in the control cultures (Fig. 1 d). No change in the viability of MCMV-infected DCs was observed after withdrawal of FCS and Flt3L (Fig. 1 d). These studies, using bone marrowderived DCs, confirmed that MCMV inhibits DC cell death.
MCMV-infected DCs are resistant to apoptosis induced by inhibition of PI3 kinase
Next we sought to determine which cellular signal transduction pathways are involved in protecting MCMV-infected DCs from apoptosis. Activation of extracellular signal regulated kinase (ERK) is required to maintain the viability of LPS-stimulated DCs after growth factor withdrawal (Rescigno et al., 1998). However, inhibition of ERK does not affect the viability of immature or LPS-stimulated DCs grown in the presence of growth factors (Rescigno et al., 1998). Similarly, inhibition of ERK kinases does not affect the viability of freshly isolated splenic DCs or human monocyte-derived DCs (Ardeshna et al., 2000; Park et al., 2002). The protection from apoptosis provided by stimulation with LPS or CpG DNA in these systems can be blocked after inhibition of the phosphatidylinositide-3-OH kinase (PI3 kinase) pathway (Ardeshna et al., 2000; Park et al., 2002).
The role of PI3 kinase activity in the context of DC survival was tested using LY294002, a specific inhibitor of PI3 kinase. MCMV-infected D1, or uninfected control D1 cells, were plated into fresh medium containing growth factor in the absence or presence of 50 µM LY294002, and the percentage of viable cells determined by annexin V-FITC staining after 16 h. Incubation of D1 DCs with LY294002 lead to reduced viability, suggesting that PI3 kinase activity is critical for maintaining DC viability (Fig. 2 a). MCMV-infected DCs were found to be resistant to the effects of LY294002, indicating that the virus can bypass the requirement for a signal from PI3 kinase in maintaining cell viability (Fig. 2 a). The contribution of the ERK MAP kinase pathway to viability was also assessed using the MEK1/2 inhibitor U0126. The viability of control or MCMV-infected DCs after inhibition of the ERK pathway was not altered (Fig. 2 a).
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Expression of Bim increases after growth factor withdrawal
We next sought to characterize the molecular events leading to apoptosis of DCs after growth factor withdrawal. Given the role of Bcl-2 proteins in regulating apoptosis induced by growth factor deprivation, we monitored the expression of Bcl-2 family members in DCs during apoptosis induced by withdrawal of growth factor. No change in the protein level of Bcl-2, Bcl-xL, or Bax was observed after growth factor withdrawal at any of the time points tested (Fig. 3 a). Similarly, no change in the expression of pro-survival Bcl-w or Bfl-1/A1 protein was detected, whereas expression of Mcl-1 was not detected in these cells (not depicted). An increase in the expression of Bim, a BH3 only pro-apoptotic protein was observed (Fig. 3 a). Alternative splicing of Bim gives rise to three variants (BimEL, BimL, and BimS) each of which contains the BH3 domain and is capable of functioning as a death inducer (O'Connor et al., 1998). Bim activity is regulated at a posttranslational level and, in healthy cells most Bim molecules are sequestered to the microtubular dynein motor complex (Puthalakath et al., 1999). Certain apoptotic stimuli result in translocation of Bim to the mitochondria where the protein is able to antagonize the function of pro-survival Bcl-2 molecules (Puthalakath et al., 1999). Therefore, the proportion of Bim protein localizing to mitochondria in DCs grown in the presence or absence of growth factor was examined by subcellular fractionation. As expected, very little Bim protein could be detected in the mitochondrial fraction of cells grown in the presence of growth factor, whereas a significant amount was observed when DCs were grown in the absence of growth factor for 12 h (Fig. 3 b). These results are consistent with observations made in lymphocyte and neuronal cultures, where Bim expression has been found to be critical for the initiation of apoptosis after growth factor withdrawal (Bouillet et al., 1999; Villunger et al., 2000; Putcha et al., 2001; Shinjyo et al., 2001).
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Knockout studies have demonstrated a redundant role for Bax and Bak, with either protein sufficient to induce apoptosis in a variety of cell types (Lindsten et al., 2000). Like Bax, induction of apoptosis results in a conformational change to the NH2 terminus of Bak and its oligomerization. Exposure of the Bak NH2-terminal epitope after MCMV infection could not be assessed because appropriate mouse reagents do not exist. Gel filtration analysis was used to determine the oligomerization status of Bak. In healthy DCs Bak eluted over a broad range (Fig. 7 c), implying that monomeric and oligomeric forms of Bak are present in normal cells, a result consistent with previous reports (Antonsson et al., 2001). After induction of apoptosis in DCs a small change in the elution profile of Bak was observed with a larger proportion of high molecular weight Bak complexes being detected. In MCMV-infected DCs Bak was predominantly contained within high molecular weight complexes even before growth factor withdrawal (Fig. 7 c). Thus, MCMV infection of DCs results in the oligomerization of both Bax and Bak.
MCMV M36, M37, and M45 do not interfere with Bax function
Three MCMV proteins (M36, M37, and M45) have been ascribed possible anti-apoptotic functions (Goldmacher et al., 1999; Brune et al., 2001; Skaletskaya et al., 2001; McCormick et al., 2003), and thus represent potential candidates for the inhibition of Bax function we observed after MCMV infection. To test the relevance of these proteins, we determined their ability to interact with Bax and to prevent apoptosis induced via the mitochondrial pathway. The viral ORFs were transfected into COS-7 cells and interaction with Bax determined by coimmunoprecipitation. Bax did not interact with either M36, M37 or M45 (Fig. 8 a). Furthermore, overexpression of these MCMV proteins did not protect from staurosporine-induced apoptosis (Fig. 8 b). The inability of the viral proteins to interact with Bax and prevent apoptotic cell death was confirmed by transfection studies in murine fibroblasts (not depicted).
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Discussion |
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The significance of Bax and Bak to the initiation of apoptosis is underscored by the finding that mice deficient for both proteins display multiple developmental defects (Lindsten et al., 2000). The key event in apoptosis mediated through mitochondrial dysfunction is a conformational change in Bax and Bak and the formation of high molecular weight complexes (Hsu et al., 1997; Nechushtan et al., 2001; Sundararajan and White, 2001). After MCMV infection, Bax was found in tight association with the mitochondrial membrane, and both Bax and Bak were in an oligomerized form. Despite both Bax and Bak displaying characteristics typically observed during apoptosis, m was not affected, nor was cytochrome c released into the cytoplasm. Importantly, a conformational change which exposes a constitutively occluded epitope at the NH2 terminus of Bax, and which normally occurs during apoptosis, was not observed in MCMV-infected DCs. Translocation of Bax to mitochondria and formation of Bax complexes without a loss of mitochondrial integrity were reported in a taxol-resistant cell line (Makin et al., 2001). The percentage of taxol-resistant cells which had oligomerized Bax, and how much Bax protein was in this conformation was difficult to determine, and indeed the majority of Bax in these cells was monomeric (Makin et al., 2001). In our MCMV-infected DC cultures all the Bax protein was oligomerized, despite the complete resistance to apoptosis. The exposure of the constitutively occluded NH2-terminal epitope of Bax observed in taxol-resistant cells (Makin et al., 2001), did not occur in MCMV-infected DCs. Our findings raise the interesting possibility that MCMV sequesters Bax and Bak in oligomeric complexes which are structurally distinct from those required to induce apoptosis. Alternatively, additional proteins may be required to cooperate with Bax/Bak oligomers to induce mitochondrial permeabilization.
Thus, MCMV infection reveals the existence of a checkpoint in the Bcl-2regulated apoptotic pathway after translocation/oligomerization of Bax and Bak. This checkpoint is specifically targeted by MCMV to inhibit apoptosis in DCs.
Given the importance of Bax/Bak in the apoptotic process it is not surprising that a number of viruses have evolved strategies to interfere with the function of these proteins. Several viruses encode Bcl-2 homologues that appear to interfere with apoptosis by directly binding to Bax/Bak and antagonizing their function (Cuconati et al., 2002). The best characterized of these is the E1B 19K protein of adenovirus (Perez and White, 2000). CMVs do not encode sequence homologues of Bcl-2, suggesting that this family of viruses has evolved alternative strategies to interfere with Bax/Bak functions. The product of the HCMV gene UL37 encodes a mitochondria-localized death suppressor termed vMIA (Goldmacher et al., 1999; Goldmacher, 2002). Recent reports have demonstrated that vMIA functions by sequestering Bax at the mitochondrial membrane in an inactive complex (Arnoult et al., 2004; Poncet et al., 2004). Although M37, the MCMV homologue of UL37, would seem an ideal candidate for mediating the observed inhibition of Bax function in MCMV-infected DCs, it should be noted that the homology between M37 and UL37 is restricted to the region encoding UL37 exon 3. Both anti-apoptotic and mitochondrial localizing functions of UL37 have been mapped to exon 1, a region lacking obvious sequence homology in the MCMV genome (Rawlinson et al., 1996; Goldmacher, 2002). Indeed, we were unable to demonstrate an association between M37 and Bax, and overexpression of M37 in COS-7 or NIH-3T3 cells did not inhibit apoptosis induced by staurosporine treatment. Similarly, coimmunoprecipitation experiments showed that Bax does not associate with either M36 or M45, the other known anti-apoptotic proteins encoded by MCMV.
Because MCMV infection of DCs results in changes to Bax similar to those induced by vMIA, we hypothesize that a functional homologue of the HCMV vMIA is encoded by MCMV. Identification of this protein is the focus of our ongoing studies. Unlike vMIA, which does not bind Bak or inhibit its function (Arnoult et al., 2004), infection of DCs with MCMV resulted in the oligomerization of both Bax and Bak. A possible explanation for this observation is that the putative MCMV vMIA homologue can interact with both Bax and Bak; alternatively, MCMV might encode an additional proteins which specifically interferes with Bak function. Finally, because activation of Bak in some cells has been shown to require Bax activation (Arnoult et al., 2004), an alternative possibility is that interference with Bax function is sufficient to prevent apoptosis in DCs.
In conclusion, we have shown that CMV interferes with apoptosis in DCs, cells that are major targets of infection and viral replication both in vitro and in vivo. The ability of MCMV to increase the life span of DCs confers the virus a significant survival advantage, because in these cells MCMV replicates to high titres for long periods of time (Andrews et al., 2001). A further exciting outcome of our studies is the clear definition of a checkpoint in the Bcl-2regulated apoptotic pathway after Bax/Bak oligomerization, but before mitochondrial disruption. Our results provide unequivocal evidence that translocation, integration, and oligomerization of Bax at the mitochondrial membrane, generally thought to represent a terminal commitment to cell death, are in fact not sufficient to force cells to die, because in MCMV-infected DCs these events occur, but the cells remain viable. Analysis of viral infections has provided many novel and important clues about complex cellular processes and pathways. Further elucidation of the mechanism used by MCMV to interfere with mitochondrial functions will undoubtedly contribute important insights into the regulation of apoptosis.
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Materials and methods |
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Preparation and purification of MCMV stocks
Mouse embryonic fibroblasts were infected with MCMV-K181-Perth salivary gland passaged stocks with an MOI of 0.05 for 1 h at 37°C. Cells were monitored daily for evidence of cytopathic effect and the supernatant removed when 100% cpe was observed. Supernatants were filtered through 0.45-µm filters and centrifuged at 35,000 g for 2 h at 4°C. The supernatant was removed, and the viral pellet resuspended in ice-cold medium, aliquoted, and stored at 180°C. Viral titers were determined on embryonic fibroblasts as described previously (Farrell et al., 1997).
Induction and detection of apoptosis
Apoptosis was induced in D1 or Flt3L expanded DCs by washing cells twice in serum-free medium. D1 DCs were reseeded in complete IMDM without R1 CM, whereas Flt3L DCs were reseeded in IMDM/1% FCS. PI3 kinase and MEK1/2 activity was inhibited with LY294002 (Calbiochem) and U0126 (Cell Signaling Technology), respectively. Apoptosis was assessed by staining cells with annexin V-FLUOS (Roche) according to the manufacturer's recommendation and cells analyzed on a FACSCalibur (Becton Dickinson). Caspase activity was determined using the caspase-3 substrate Ac-DEVD-pNA (Calbiochem) according to the manufacturer's instructions. Apoptosis was induced in transfected COS-7 cells by staurosporine treatment (250 nm, 18 h). Viability was assessed by trypan blue exclusion.
Antibodies
The following antibodies were used for this work: anti-Grb2 (clone C-23) obtained from Santa Cruz Biotechnology; antiBfl-1/A1 (clone 78616.11) obtained from R&D Systems; antiCox IV (clone 20E8) obtained from Molecular Probes; anticytochrome c (7H8.2C12), antiBcl-x (clone 44), and anti-Bax (6A7) obtained from BD Biosciences; anti-Bax (clone 5B7) obtained from Sigma-Aldrich; and anti-Bax and anti-Bak obtained from Upstate Biotechnology. The antiBcl-2 (clone 3F11), anti-Bim (clone 14A8), and antiBcl-w antibodies were gifts from D. Wang and L. O'Reilly (Walter and Eliza Hall Institute, Melbourne, Australia). Antimouse and antirabbit peroxidase conjugates were purchased from Amersham Biosciences; the antirat and antiArmenian hamster peroxidase conjugates were obtained from Jackson ImmunoResearch Laboratories.
Preparation of cell lysates and subcellular fractionation
Total cell lysates were prepared by resuspending cells in Triton X-100 lysis buffer (150 mM NaCl, 10 mM Tris, pH 7.4, 1% Triton X-100, 1x complete protease inhibitors [Roche]) and incubating on ice for 30 min. Nuclei and cell debris was removed by centrifugation (1,000 g for 5 min) and protein concentration determined using a Micro BCA assay kit (Pierce Chemical Co.). Cytoplasmic and mitochondrial fractions were prepared by resuspending 107 cells in 500 µl isotonic lysis buffer (210 mM mannitol, 70 mM sucrose, 20 mM Hepes, pH 7.2, 10 mM KCl, 1.5 mM MgCl2, 1 mM EGTA, 1x complete protease inhibitors) and passing the sample through a G27 needle 30 times. The resulting sample was centrifuged at 1,500 g for 5 min to remove nuclei and unbroken cells. The supernatant was subsequently centrifuged at 14,000 g for 10 min. The pellet, containing mitochondria was resuspended in Triton X-100 lysis buffer, while the supernatant was centrifuged at 100,000 g for 45 min at 4°C with the resulting supernatant used as the cytoplasmic fraction.
Alkali extraction of mitochondria
Mitochondria from healthy or apoptotic cells were isolated as described above and resuspended in freshly prepared 0.1 M NaCO3, pH 11.5, and incubated for 20 min on ice. Mitochondrial membranes were pelleted by centrifugation (100,000 g for 2 h at 4°C). The resulting pellet (alkaliresistant fraction) was resuspended in Triton X-100 lysis buffer, while the supernatant contained alkalisensitive proteins.
Immunoblotting
Protein samples were resolved on 412% Bis-Tris gradient gels (Novex) and transferred to PVDF plus membrane (Osmonics). Immunodetection was performed using the appropriate peroxidase-conjugated secondary antibody and ECL detection reagent (Amersham Biosciences). Equal loading of lanes was confirmed by Grb2 or Cox IV immunoblot.
Confocal microscopy
Analysis of m was performed by resuspending cells (3 x105 cells/ml) in medium containing 300 nM MitoTracker red CMXRos in the presence or absence of 50 µM CCCP for 20 min at 37°C. Cells were then washed in medium twice and plated onto silane-(ICN Biomedicals)-coated coverslips and incubated at 37°C for 20 min. Cells were fixed in 3.7% PFA (37°C for 15 min), and washed twice in PBS. Cells were permeabilized with PBS/0.2% Triton X-100 and stained with 2 µM Hoechst 33258 to visualize nuclei and mounted. All analysis was performed on an MRC 1000/1024 UV laser scanning confocal microscope (Bio-Rad Laboratories) with a 40x objective (NA 1.3; Nikon) and images saved as Biorad.PIC files. Subsequent analysis was performed using Adobe Photoshop v. 7.0.
Gel filtration
Gel filtration was performed at 4°C using Toyopearl HW-65F resin (Tosoh Bioscience) equilibrated in column buffer (25 mM Hepes-NaOH, pH 7.5, 300 mM NaCl, 200 µM DTT, 2% CHAPS). The column was calibrated using ferritin (440 kD), BSA (67 kD), and trypsin inhibitor (20.1 kD). The void volume of the column was determined using blue dextran. Mitochondria were isolated by differential centrifugation as outlined above and solubilized using CHAPS lysis buffer (150 mM NaCl, 25 mM Hepes, pH 7.5, 2% CHAPS, 1x complete protease inhibitors). The sample was then centrifuged at 21,000 g for 20 min to remove insoluble material. Samples of 500 µl were loaded onto the column and the eluate was monitored at 280 nm. Fractions of 25 drops (680 µl) were collected and aliquots analyzed by immunoblot.
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Acknowledgments |
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This project was supported by grants from the National Health and Medical Research Council of Australia and the Wellcome Trust. M.A. Degli-Esposti is supported by a Wellcome Trust Overseas Senior Research Fellowship in Biomedical Science.
Submitted: 1 March 2004
Accepted: 27 July 2004
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