Article |
Address correspondence to Ramanujan S. Hegde, Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, 18 Library Drive, Building 18, Room 101, Bethesda, MD 20892. Tel.: (301) 496-4855. Fax: (301) 402-0078. E-mail: hegder{at}mail.nih.gov
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Abstract |
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Key Words: protein translocation; endoplasmic reticulum; secretion; translocon; signal sequence
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Introduction |
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Notwithstanding this multifunctionality, the Sec61p complex is insufficient for cotranslational translocation. Although the Sec61p complex can recognize signal sequences, efficient nascent chain targeting to the membrane and transfer to the translocation channel additionally requires the coordinated actions of the cytosolic signal recognition particle and its membrane-bound receptor (SR)* (Walter and Johnson, 1994). After targeting to the mammalian ER, another component, TRAM, is necessary for the efficient translocation of most substrates in a manner dependent on structural features of their signal sequences (Gorlich et al., 1992a; Voigt et al., 1996). Thus, reconstitution into lipid vesicles of at least three proteins, the Sec61p complex, SR, and TRAM, is necessary for the translocation of most substrates, defining them as essential translocon components (Gorlich and Rapoport, 1993).
The translocation of several model secretory and membrane proteins has been tested in proteoliposomes containing these three purified components (Gorlich and Rapoport, 1993; Voigt et al., 1996). Although every substrate examined thus far displays at least some level of translocation, the efficiency of transport relative to unfractionated microsomes appears to vary from protein to protein. For instance, the translocation of the secretory hormone prolactin (Prl) into the minimal system approached 6070% of that observed in unfractionated starting microsomes (Gorlich and Rapoport, 1993). By contrast, translocation of the hormone
-factor in the same proteoliposomes was only
1520% of the control, raising the possibility that its transport may require stimulatory factor(s) not needed by Prl. Unfortunately, the difficulties of functionally assaying modestly stimulatory activities using laborious and technically demanding membrane protein reconstitution methods have hindered a biochemical approach to the identification of any such factors.
More recently, a particularly striking example of discrepant translocation in native versus minimal proteoliposomes was described for the prion protein (PrP). In native microsomes, the majority of PrP is ordinarily fully translocated into the lumen in a form termed secPrP, with smaller amounts being made as single spanning membrane proteins in either orientation, termed CtmPrP (a type II or Cout/Nin orientation) and NtmPrP (a type I or Cin/Nout orientation) (Hegde et al., 1998a; Holscher et al., 2001; Stewart and Harris, 2001). However, only the CtmPrP form, whose increased expression is associated with neurodegenerative disease (Hegde et al., 1998a), is made to any appreciable degree in proteoliposomes containing the minimal translocation machinery (Hegde et al., 1998b). Thus, although the currently identified essential translocation machinery is able to mediate a basal level of translocation for many substrates (Gorlich and Rapoport, 1993; Voigt et al., 1996), optimal and proper transport of the entire repertoire of proteins that ordinarily transit the secretory pathway is likely to involve other stimulatory components that act in a substrate-specific manner (Andrews and Johnson, 1996; Hegde and Lingappa, 1999). The identity of such putative factors, the attributes of the substrates for which they are important, or the specific steps in translocation at which they might act all remain elusive.
In this study, we have exploited the inability of PrP to be translocated properly by the minimal translocation machinery to facilitate the purification of a trans-acting stimulatory factor involved in its translocation. We subsequently demonstrate that this factor, identified as the translocon-associated protein (TRAP) complex, is more broadly involved in substrate translocation in a manner dependent on functional features of signal sequences. Our results therefore identify an additional functional component of the translocation machinery, define the characteristics of the substrates that depend on its function, and indicate that it acts at the critical step of initiation of translocation.
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Results |
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This property was exploited to prepare a total detergent extract depleted of the very minor population of polypeptides (<5% of total protein; unpublished data) that bind Q-sepharose under these high salt conditions (hereafter termed a Q-depleted extract). Proteoliposomes reconstituted from a Q-depleted extract contained the full complement of SR, Sec61p complex, TRAM, ribophorin I (a component of the oligosaccharyl transferase complex), and signal peptidase complex (Fig. 1 C). Yet, they were essentially devoid of TrAF activity, being largely inactive in generating secPrP (Fig. 1 D, lane 2). This defect was fully replenished upon coreconstitution of the Q-depleted extract with the proteins eluted from Q-sepharose (Fig. 1 D, lane 3). Importantly, proteoliposomes made from the Q-depleted extract showed no discernible defect in the translocation of Prl (Fig. 1 D, bottom), consistent with this substrate needing only SR, Sec61p complex, and TRAM for maximal translocation (Gorlich and Rapoport, 1993). Thus, a TrAF activity that is involved in secPrP translocation, but not required for Prl translocation, could be separated from the currently established essential components of the translocon with Q-sepharose, thereby facilitating its subsequent purification.
Purification and identification of TrAF as the TRAP complex
Elution of the proteins that bound to Q-sepharose with a step gradient of salt, followed by fractionation with ConA, consistently resulted in the substantial enrichment of only five main proteins (Fig. 2 A). These proteins could be identified by immunoblotting, respective sizes, and abundance to be calnexin (CNX) and the through
subunits of the TRAP complex (unpublished data; Fig. 2 B). Each fraction was then coreconstituted with a Q-depleted extract into proteoliposomes that were analyzed by immunoblotting (Fig. 2 B) and translocation activity (Fig. 2 C). Whereas Prl translocation was comparable in each of the proteoliposomes, secPrP translocation was diminished by Q-depletion and replenished to varying degrees by the different fractions (Fig. 2 C). We consistently noticed that the highest levels of secPrP translocation corresponded to the fractions containing TRAP, and to a lesser extent CNX (Fig. 2, compare B with C).
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To test whether the tetrameric TRAP complex alone was the component that stimulates secPrP translocation, we took advantage of its known tight association with the ribosome (Gorlich et al., 1992b; Matlack and Walter, 1995) to separate it from any contaminating CNX. Anion exchange chromatography of a ribosome-associated membrane protein (RAMP) fraction (Gorlich and Rapoport, 1993) resulted in a TRAP preparation (Fig. 2 D) in which CNX was undetectable by immunoblotting (unpublished data). A titration of this RAMP-purified TRAP showed that it could restore secPrP translocation to levels comparable to those seen when a Q-depleted extract was replenished with the total Q eluate (Fig. 2, E and F). These data suggest not only that TRAP is able to stimulate secPrP translocation, but also that it is likely to be the principal, if not only, active component that is depleted by Q-sepharose. Thus, by two independent methods of purification, fractions substantially enriched for TRAP are active in stimulating PrP translocation. We therefore conclude that TrAF, the activity originally defined by its substrate-specific requirement for PrP translocation and topogenesis but not for Prl translocation (Hegde et al., 1998b), is the tetrameric TRAP complex.
Dependence on TRAP for translocation is influenced by the signal sequence
We next sought to determine the basis for the substrate-specificity of TRAP function that allows Prl, but not PrP, to be translocated completely independently of TRAP. Because the most apparent difference between Prl and PrP is that the latter, but not the former, is synthesized in multiple topologic forms, we analyzed the behavior of a PrP mutant that eliminates this property. PrP(G123P), which disrupts the potential membrane-spanning domain in PrP, is made exclusively in the secPrP form (Hegde et al., 1998a) and is therefore topologically equivalent to Prl. When analyzed in proteoliposomes lacking and containing TRAP, we found that the translocation of PrP(G123P) was influenced by the presence of TRAP in a manner similar to the secPrP form of wild-type PrP (Fig. 3 B). Similar results were observed with other mutants of PrP that abolished or diminished its topologic heterogeneity (unpublished data), arguing that the topologic heterogeneity of PrP is unlikely to be the basis of its requirement for TRAP.
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Correlation of TRAP dependence with post-targeting signal sequence function
The PrP and Prl signal sequences differ in numerous physical and functional characteristics, including length, hydrophobicity, charge, amino acid composition, and effect on substrate translocation (Rutkowski et al., 2001; Kim et al., 2002). To gain insight into whether any of these features influence dependence on TRAP for translocation, we took advantage of a series of constructs encoding each of eight mammalian signal sequences fused to the PrP mature domain (Kim et al., 2002). A comparative analysis of these natural signal sequences revealed that the translocation of constructs containing the signals from Prl, growth hormone (GH), and osteopontin (Ost) was influenced to a lesser degree than PrP by the presence or absence of TRAP (Fig. 4 A). By contrast, the other four signals analyzed displayed more dependence on TRAP for directing translocation of the PrP mature domain.
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The sequences of the eight signals analyzed for TRAP dependence were compared by several criteria to elucidate which, if any, physical property may be an important determinant (Fig. 5). However, no single feature of the signal sequence correlated with their respective functional dependence on TRAP. This included overall length, maximal hydrophobicity, length of the hydrophobic domain, charge of the n-domain (the nonhydrophobic domain preceding the hydrophobic core of the signal; von Heijne, 1985), or charge differential across the hydrophobic domain. A qualitative assessment of the hydropathy profiles of the signals also did not reveal a simple correlation. And finally, a systematic difference was not observed in the amino acid composition of the hydrophobic core of the signal (distinguishing between leucine/isoleucine/valine versus alanine/phenylalanine/tryptophan). Together, these observations suggest that TRAP dependence is determined by either a combination of multiple physical features of the signal sequence or by a feature that was not analyzed here.
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We first analyzed progressively longer translocation intermediates of Prl containing either its own or the PrP signal sequence. At translocation intermediates of only 30 residues beyond the signal sequence, both Prl and PrPPrl displayed incomplete salt-resistant binding and complete exposure to cytosolic protease (Fig. 7 A). At an intermediate that was 20 residues longer, Prl displayed quantitative salt-resistant binding and was well shielded from cytosolic protease. By contrast, PrPPrl, while bound to the ER in a salt-resistant manner, was observed to largely be accessible to cytosolic protease. Only after the synthesis of an additional 20 residues did the PrPPrl intermediate display both salt- and protease-resistant binding comparable to that seen for Prl (Fig. 7 A). At subsequent lengths, Prl and PrPPrl appeared to be similar in their salt- and protease-resistant binding characteristics (unpublished data), and synthesis of the full-length products resulted in the translocation of both substrates (Fig. 7 A).
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We also performed a similar analysis of the various signal sequence constructs from Fig. 4 A. Translocation intermediates of each construct containing 101 amino acids beyond the signal sequence were generated and assayed for salt-resistant binding, cytosolic accessibility to protease, and ability to translocate into the lumen upon release from the ribosome (e.g., as diagrammed in Fig. 7 C). Although each of the nascent chain intermediates was comparably membrane bound in a salt-resistant manner (Fig. 7 D, lanes 3 and 4), substantial differences were seen in their protease accessibility (lane 5) and translocation competence (lanes 68). The translocation intermediates containing the Prl, GH, or Ost signal sequences were protected from protease digestion and translocated into the lumen upon puromycin release to a greater degree than the other translocation intermediates.
Because shielding of the nascent chain from the cytosol and commitment to translocation into the lumen are both measures of a properly gated translocon, these data are consistent with and corroborate the previous analysis of signal gating activity based on topogenic sequence competition (Kim et al., 2002). More importantly, these observations provide specific insight into the characteristics of TRAP-dependent and -independent signals. Signals that interact strongly with the translocon to mediate efficient formation of a protease-protected ribosomemembrane junction and achieve a translocation-committed state early in synthesis tend to be TRAP independent. By contrast, TRAP-dependent signals are characterized by their weaker interactions with the translocon, as evidenced by their relatively poor ability to commit the nascent chain to forward translocation at an early stage in synthesis.
It is additionally worth noting that although the signal is a principal determinant of a substrate's efficiency in initiating translocation, a role for the mature domain should not be entirely excluded. Indeed, in comparing translocation intermediates of the same signals fused to different mature domains, some differences in the timing of the achievement of protease resistance and commitment to translocation have been observed (e.g., Fig. 7, A versus D; unpublished data). It may be that sequences in the initial portion of the mature domain also influence signal sequence gating and translocation activities (Kim et al., 2002), although this remains to be investigated in a systematic manner. Regardless of this possibility, when the mature domain is held constant (as in Fig. 7 D), differences can readily be demonstrated between signal sequences in their post-targeting gating function, and this activity is inversely correlated with its dependence on TRAP for translocation.
If this functional feature of the signal sequence is the basis of its TRAP dependence, we reasoned that it should be manipulable in predictable ways. To test this, we analyzed the TRAP dependence of two point mutations in the PrP signal sequence (termed N7 and N9 [Fig. 8 A], which replace a leucine at position 4 with either an aspartate or arginine, respectively). The N7 and N9 mutations were shown previously to decrease and increase, respectively, the post-targeting gating function of the PrP signal (Kim et al., 2002). In addition, a recent analysis of serial translocation intermediates revealed that the N9 signal mediates nascent chain protection from the cytosol and access to the ER lumen at an earlier stage in translocation than the N7 signal (Kim and Hegde, 2002). As would be predicted from our working hypothesis above, we found that when fused to Prl, the N9, but not N7, signal substantially reduced TRAP dependence (Fig. 8 B). Thus, a single amino acid mutation that increases the strength of a signal sequence's post-targeting interaction with the translocon is sufficient to decrease TRAP dependence. Taken together with the analysis of natural signal sequences, these data argue that TRAP is particularly important for the translocation of substrates whose signal sequences interact relatively weakly with the native translocon and are thus less efficient at directing closure of the ribosometranslocon junction and committing the nascent chain to forward translocation early in biogenesis.
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Discussion |
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The conclusions of our study can be readily reconciled with earlier work suggesting that TRAP may not be functionally essential for protein translocation. In one study, proteoliposomes reconstituted from a detergent extract immunodepleted of TRAP had failed to reveal a translocation defect (Migliaccio et al., 1992). Because Prl was the principal substrate analyzed by Migliaccio et al. (1992), the inability to detect a consequence of TRAP depletion is consistent with our finding that it contains a TRAP-independent signal sequence. In other studies, reconstitution with purified components that did not include TRAP still supported translocation of numerous model proteins, albeit to varying degrees of efficiency (Gorlich and Rapoport, 1993; Voigt et al., 1996). This can be explained by our observation that for most substrates, the requirement for TRAP is not absolute and varies in a signal sequencespecific manner. Indeed, we have recently confirmed that -factor is at least partially TRAP dependent (unpublished data), providing an explanation for its relatively poor translocation (in comparison to Prl) into proteoliposomes containing only the Sec61p complex, SR, and TRAM (Gorlich and Rapoport, 1993). Thus, while our study clearly demonstrates a role for TRAP in translocation, its modest stimulatory effect on the vast majority of substrates may partly explain why its function had previously remained enigmatic and difficult to demonstrate.
Although the molecular mechanism of TRAP function is not clear at present, the role of the signal sequence in determining TRAP dependence suggests that TRAP is likely to improve transport by acting at a signal-mediated step in translocation. The demonstration that proteoliposomes lacking TRAP are able to mediate targeting and salt-resistant binding of both TRAP-dependent (PrP) and -independent (PrP) substrates with equal efficiency (Hegde et al., 1998b) argues against a role for TRAP in modulating targeting. Instead, TRAP is likely to influence the signal sequence's post-targeting function of gating the translocation channel to initiate substrate translocation into the ER. However, TRAP does not appear to be directly involved in signal sequence recognition. Extensive site-specific cross-linking studies using probes in the signal sequence have demonstrated that it interacts with the translocon at a specific binding site at the membrane formed by a combination of the Sec61p complex, TRAM, and membrane lipids (High et al., 1993; Jungnickel and Rapoport, 1995; Martoglio et al., 1995; Voigt et al., 1996; Mothes et al., 1998). Only at substantially later steps in translocation are cross-links seen between TRAP and the nascent chain, probably to regions of the mature domain (Gorlich et al., 1992a; Mothes et al., 1994).
If TRAP does not interact with the signal directly, how then might it influence translocation in a signal-dependent manner? In one model, TRAP could interact with the mature domain of the nascent chain on the lumenal side of the translocon to help stabilize the newly inserted nascent chain in the proper orientation at the translocation channel. Thus, signals whose strong interaction with Sec61 and/or TRAM allow the nascent chain to be firmly held in the proper "loop" orientation would not additionally need TRAP to stabilize the mature domain. This may explain why the strength of signaltranslocon interactions early in translocation is a key determinant of TRAP dependence. Consistent with such a model, the
and ß subunits of TRAP have large, conserved lumenal domains (Hartmann et al., 1993), cross-links to TRAP
are not seen until nascent chain lengths are relatively long (Gorlich et al., 1992a; Mothes et al., 1994), and mutations in a signal's n-domain, which can modulate interactions with TRAM, can influence TRAP dependence (Fig. 8).
An alternative model is one in which TRAP stabilizes the nascent chain indirectly by an effect on Sec61p structure, function, or oligomerization. Given that TRAP appears to be as abundant as Sec61p and is found tightly associated with membrane-bound ribosomes (Gorlich et al., 1992b; Matlack and Walter, 1995), it would seem reasonable to conclude that at least one copy of TRAP is present at every translocon. These observations, coupled with the fact that the "native" translocon appears to be larger and structurally different than a translocon composed only of the Sec61p complex (Hanein et al., 1996; Menetret et al., 2000), raises the possibility that incorporation of TRAP into a Sec61p translocon changes its overall dimensions. Perhaps the slightly larger translocon, with a bigger and differently shaped pore, is more easily able to accommodate certain nascent chains than the more restrictive translocon lacking TRAP. This could have the consequence of allowing nascent chains easier access to the ER lumen, where they can either be partially folded or perhaps interact with lumenal chaperones, thereby biasing their forward transport. Substrates that are tightly inserted into the translocation site and do not have access to the cytosol (due to a closed ribosometranslocon junction) would not need lumenal chaperones or protein folding to bias translocation, perhaps explaining why they do not require TRAP for efficient transport. Future studies examining the consequences of TRAP on the structure and/or function of the Sec61p channel will be required to address these issues.
From a physiological standpoint, it is particularly noteworthy that dependence on the function of accessory factors (such as TRAP and TRAM) is influenced to varying degrees by topogenic elements, such as the signal sequence, that are highly divergent from one substrate to the next. This raises the intriguing possibility that modulation of the machinery that is involved in signal recognition or function at the ER could be exploited by the cell to regulate translocation (and hence secretion) in a substrate-specific manner. It is tempting to speculate that the observed phosphorylation of TRAP (Prehn et al., 1990; Ou et al., 1992) and other translocon components (Gruss et al., 1999) may play yet unappreciated regulatory roles in translocation. Similarly, our discovery of a key role for TRAP during PrP translocation could have potential implications for disease pathogenesis, given that defects in the topogenesis of PrP lead to the development of neurodegeneration (Hegde et al., 1998a, 1999). Thus, substrate-specific translocation factors, in contrast to the core translocation machinery, are more likely to be involved in either physiologic regulation and/or disease pathogenesis because modulation of their function may influence the biogenesis of a relative minority of secretory and membrane proteins.
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Materials and methods |
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Fractionation and reconstitutions
Saponin-extracted, EDTA- and high saltwashed RMs were prepared as previously described (Hegde et al., 1998b) and resuspended at 1 eq/µl (see Walter and Blobel, 1983, for definition) in EB (350 mM KAc, 50 mM Hepes, pH 7.4, 5 mM MgCl2, 15% glycerol, 5 mM 2-mercaptoethanol, and a protease inhibitor cocktail [EDTA-free complete inhibitor; Roche Applied Sci.]). DBC was added to 0.8% from a 10% wt/vol stock solution and particles larger than 30s were removed by centrifugation in a TL100 ultracentrifuge (Beckman Instruments) to yield a DBC extract of RMs. Glycoprotein-depleted extract was prepared by incubation, with gentle mixing, in batch at 4°C for 812 h of the DBC extract with ConA sepharose (equilibrated in EB) at a ratio of 150 µl packed ConA beads per 1,000 eq DBC extract. Q-depleted extract was prepared using 150 µl Q-sepharose Fast Flow (equilibrated in EB) per 1,000 eq DBC extract, incubated in batch for 412 h at 4°C. Prior to elution, resins were washed four times with 610 volumes each with EB containing 0.5% DBC. Elution of glycoproteins from ConA was performed by incubation at 25°C for 90 min in 500 mM KAc, 50 mM Hepes, pH 7.4, 10 mM EDTA, 0.25 M
-methyl-mannopyrannoside, 15% glycerol, 5 mM 2-mercaptoethanol, 0.5% DBC. Elution of proteins from Q-sepharose was performed in EB containing 0.5% DBC and the appropriate KAc concentration, as indicated in the figure legends. Reconstitutions of extracts into proteoliposomes was typically performed in a 200-µl volume containing 100 µl at 1 eq/µl of either a DBC extract, glycoprotein-depleted extract, or Q-depleted extract. The remainder of the volume was used to add crude protein fractions and/or purified proteins and contained between 300 and 1,000 mM KAc, 50 mM Hepes, pH 7.4, 15% glycerol, and 0.5% DBC. Eluates from ConA also contained up to 10 mM EDTA and 0.25 M
-methyl-mannopyrannoside. EB containing 0.5% DBC was used to bring the final volume to 200 µl as needed, after which 100 µg of lipids (from a 20 mg/ml stock solution) was added, and the entire mixture was added to 150 mg Biobeads SM2 prepared as described above. After incubation at 4°C with mixing for 1218 h, the fluid was separated from the beads and diluted to 1 ml with ice cold water, and the proteoliposomes were sedimented at 70,000 rpm for 15 min in a TL100.3 rotor with microcentrifuge tube adaptors. The pellets were resuspended in a final volume of between 20 and 40 µl, depending on the experiment, in 50 mM Hepes, pH 7.4, 100 mM KAc, 2 mM MgCl2, 250 mM sucrose, 1 mM DTT, frozen in liquid nitrogen, and stored at -80°C until their use in translocation reactions. Typically, 0.51 µl of these proteoliposomes was used per 10-µl translation reaction.
Purification of TRAP
A dialyzed RAMP fraction was prepared from 30,000 equivalents of pig pancreas microsomes exactly as described previously (Gorlich and Rapoport, 1993). This was bound to a 6-ml column of Q-sepharose Fast Flow equilibrated in 20 mM Hepes, pH 7.6, 5 mM MgAc2, 1% digitonin, 5 mM 2-mercaptoethanol, washed with 20 ml of equilibration buffer, and step eluted with 10 ml of 1,000 mM KAc, 50 mM Hepes, pH 7.6, 2.5% digitonin, 10% glycerol, 5 mM 2-mercaptoethanol. The peak fractions of this crude TRAP fraction (
3.5 ml) were pooled and frozen in aliquots in liquid nitrogen and stored at -80°C until needed for reconstitutions. Prior to its use in reconstitutions, 500 µl of the crude TRAP was diluted with an equal volume of 50 mM Hepes, pH 7.4, 5 mM MgCl2, 15% glycerol, bound in batch (60 min at 4°C with mixing) to 5070 µl Q-sepharose Fast Flow, and washed twice with 1 ml each of EB containing 0.5 M KAc and 0.5% DBC to exchange the detergent and remove contaminants. Pure TRAP was subsequently eluted in sequential 250-µl steps of EB containing 600, 700, 800, 900, and 950 mM KAc and 0.5% DBC. Peak fractions were pooled and used in reconstitutions as described above. The protein profile of these final purificationdetergent exchange steps is shown in Fig. 2 D. This RAMP-purified TRAP was used for the replenishment of Q-depleted proteoliposomes analyzed in Figs. 3, 4, 6, and 8.
Cell-free translocation assays
In vitro transcription with SP6 or T7 RNA polymerase, translation in rabbit reticulocyte lysate, and translocation using dog pancreas RMs have been described previously (Hegde et al., 1998b and references therein). The analysis of PrP and Prl translocation and topology in RMs and reconstituted proteoliposomes by protease protection was as previously described (Hegde et al., 1998b). Truncated transcripts lacking a stop codon were prepared in one of two ways. For the serial truncations in Fig. 7 A, 5' and 3' oligos annealing to the SP6 promoter and the site of truncation, respectively, were used to PCR amplify the appropriate segment of the plasmids (using Pfu polymerase; Stratagene) before transcription. Alternatively, truncations in Fig. 7 (B and D) used Pvu2-digested plasmids (which cleaves at codon 56 of mature Prl) and EcoO109-digested plasmids (which cuts at codon 101 of mature PrP), respectively. Translation reactions encoding truncated products were synchronized by the addition of aurin tricarboxylic acid after 5 min at 26°C, after which translation was allowed to proceed for an additional 20 min at 26°C. Translocation was performed by either including RMs (at 0.1 eq/µl) in the translation reaction (Fig. 7 A) or adding them (at 0.3 eq/µl) after synthesis of the ribosomenascent chains and incubating an additional 15 min at 26°C. Sedimentation analysis for salt-resistant binding was performed at 4°C by diluting translation reactions (usually 5 µl) with 20 volumes of 50 mM Hepes, pH 7.4, 500 mM KAc, 5 mM MgCl2, layering onto a 100-µl cushion of the same buffer containing 0.5 M sucrose, and centrifugation at 70,000 rpm for 5 min at 4°C in a Beckman TL100.3 rotor with microcentrifuge tube adapters. The supernatants were removed by aspiration before subsequent analysis of the pellets. Protease protection of truncated nascent chains was with 0.5 mg/ml proteinase K (PK) on ice for 60 min as described previously (Hegde et al., 1998b). Release of nascent chains with puromycin and high salt was performed by adjusting the translation reaction to 0.5 M KAc and 1 mM puromycin before incubation at 26°C for 15 min. In many of the experiments shown, translocated products were immunoprecipitated with the appropriate antibodies against the translation products because many of the truncated products and the NtmPrP and CtmPrP fragments migrate close to the highly abundant endogenous globin in reticulocyte lysate, which often distorts that region of the gel.
Miscellaneous
Separation of proteins by SDS-PAGE was on 12% Tris-tricine gels. Immunoblotting was performed after transfer to nitrocellulose. Primary antibodies were diluted between 1:2,000 and 1:10,000. HRP-conjugated secondary antibodies were used at 1:5,000, and the blot was developed with SuperSignal chemiluminescence reagents from Pierce Chemical Co. Quantitative analysis of translocation experiments used a Molecular Dynamics phosphorimager with accompanying software, whereas autoradiographs made on Kodak BioMax film were digitized for display in the figures (prepared using Adobe Photoshop® and Adobe Illustrator®).
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Footnotes |
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Acknowledgments |
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This work was supported by the Intramural Research Program of the National Institutes of Health.
Submitted: 17 October 2002
Revised: 30 December 2002
Accepted: 30 December 2002
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References |
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