Article |
Address correspondence to J.V. Small, Dept. of Cell Biology, Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, Salzburg 5020, Austria. Tel.: 43-662-63961-11. Fax: 43-662-63961-40. E-mail: jvsmall{at}imb.oeaw.ac.at
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Abstract |
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Key Words: microtubules; kinesin; actin cytoskeleton; adhesion; signalling
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Introduction |
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Apart from causing cell depolarization, microtubule disruption in fibroblasts leads, via the activation of Rho, to the enlargement or induction of actin stress fiber bundles and associated focal adhesions (Lloyd et al., 1977; Bershadsky et al., 1996; Enomoto, 1996), as well as to a parallel increase in actomyosin contractility (Danowski, 1989). Contractility in the actin cytoskeleton, mediated by myosin II, was independently shown to be required for the development and maintenance of focal adhesion sites induced by Rho (Chrzanowska-Wodnicka and Burridge, 1996). According to these findings, it was suggested that microtubule-mediated processes may suppress contractility, and via this route influence both substrate anchorage and anchorage-linked signaling events (Bershadsky et al., 1996).
A direct link between microtubules and the substrate adhesion machinery, suggested in earlier studies (Rinnerthaler et al., 1988), was confirmed by the demonstration of specific, spatial interactions between microtubules and substrate adhesions in living cells (Kaverina et al., 1998, 1999). In these studies it could be shown that adhesion site turnover or detachment, leading in the latter case to the retraction of a cell edge, was associated with microtubule-targeting events (Kaverina et al., 1999). The same mode of retraction could be mimicked by the local application of inhibitors of myosin-dependent contractility; therefore, it was proposed that microtubules mediate the point delivery of a relaxing factor(s) to adhesion sites that promotes their disassembly (Kaverina et al., 1999, 2000). In this scenario, the possibility that microtubules may alternatively sequester a factor that potentiates contractility and adhesion site growth (Enomoto, 1996) could not be excluded. In either case, cell polarization was explained by the spatially regulated disassembly of adhesion sites, effected via the selective targeting of "dispensable" adhesions by microtubules (Kaverina et al., 1999, 2000).
Because microtubule motors (Kamal and Goldstein, 2000; Terada and Hirokawa, 2000) would be the logical carriers of a putative factor to or from adhesion sites, in this study we have investigated the consequences of inhibiting kinesin and dynein activity on adhesion site dynamics. Strategies for inhibiting microtubule motorlinked activities include the use of probes that either block motor activity, interfere with the association of cargo with the motor, or that compete with motors in microtubule binding. Of these, effective in vivo means of disrupting the trafficking mediated by conventional kinesin (Kinesin-1 or KIF5) (Terada and Hirokawa, 2000) include the injection of function-blocking antibodies (Ingold et al., 1988; Brady et al., 1990; Hollenbeck and Swanson, 1990; Rodionov et al., 1993; Lippincott-Schwartz et al., 1995; Bi et al., 1997; Tuma et al., 1998), the overexpression of kinesin motor domain mutants that bind irreversibly (Nakata and Hirokawa, 1995) or not at all (Wubbolts et al., 1999) to microtubules, and the overexpression of Tau protein (Ebneth et al., 1998). To this list can be added the displacement of at least some cargo by the overexpression of truncated kinesin light chains (Verhey et al., 2001). Dynein-mediated activities have been disrupted by antibodies directed to the dynein intermediate chain (Steffen et al., 1997) or by disruption of the dynein-associated dynactin complex that is required by dynein for cargo binding (Karki and Holzbaur, 1999). Dynactin disruption is readily achieved by the overexpression of the dynactin subunit, dynamitin (Burkhardt et al., 1997).
In most of our earlier work, we employed fish fibroblasts for correlated studies of microtubule and adhesion site dynamics (Kaverina et al., 1998, 1999, 2000). However, because the antibodies used to block kinesin activity were ineffective in fish, the major part of the present studies was performed on Xenopus fibroblasts. As we show, microtubule-adhesion site targeting also occurs in these cells, with the same consequences on adhesion site turnover as demonstrated in fish cells. We demonstrate that kinesin and not dynein is required for the modulation of adhesion site dynamics by microtubules. It is further shown that kinesin inhibition has no effect on either the targeting of adhesion sites by microtubules or on microtubule dynamics, and therefore that targeting and signal transmission are independent processes.
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Results |
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Major changes in the size and distribution of substrate adhesion sites in fibroblasts occur after the disassembly of microtubules by colchicine or nocodazole (Bershadsky et al., 1996; Enomoto, 1996; Kaverina et al., 1997, 1999). As shown in Fig. 1, microtubule disassembly by nocodazole resulted in the growth of adhesion sites over a 3-h period, as well as in the characteristic depolarization of cell shape that has been seen in other fibroblast types. Measurement of contact size and number in vinculin-injected cells imaged at zero time and 3 h later confirmed the dramatic shift toward larger and fewer adhesion sites in nocodazole (Fig. 2). Time-lapse imaging of the same cells revealed the dynamics of adhesion site reorganization toward the depolarized state. Accordingly, adhesion site growth occurred by the enlargement of single adhesions as well as by the fusion of adhesions by sliding (Video 8, available at http://www.jcb.org/cgi/content/full/jcb.200105051/DC1). Both of these effects are attributable to a depolarized equalization and an increase in contractility in the actin cytoskeleton (see Discussion) (Danowski, 1989; Riveline et al., 2001).
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Adhesion site dynamics are unaffected by a block in the dyneincargo interaction
To investigate the possible role of dynein in microtubule-linked signaling to adhesion sites, we used two methods to block dyneincargo interactions: (a) injection with a function-blocking antibody against the dynein intermediate chain (Steffen et al., 1997); and (b) transfection with dynamitin (Burkhardt et al., 1997). An indicator of a block in dynein-linked activity is the dispersion of lysosomes to the cell periphery (Burkhardt et al., 1997). As shown in Fig. 3 (Video 1, available at http://www.jcb.org/cgi/content/full/jcb.200105051/DC1), injection of cells with the m742 anti-dynein antibody caused the efficient scattering of rhodamine dextranloaded lysosomes away from the centrosomal region, consistent with a block of dynein activity in the Xenopus cells. This effect was observed in all injected cells. Under the same conditions, no change in the general distribution or dynamics of microtubules was observed in the antibody-injected cells, as compared with controls (unpublished data).
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Control experiments showed that injection of the SUK-4 antibody consistently induced the collapse of mitochondria into the perinuclear area (Fig. 5) diagnostic of kinesin inhibition (Rodionov et al., 1993).We then investigated the influence of kinesin inhibition in cells injected with rhodamine-vinculin. As shown in Fig. 6, A and B, a block in kinesin activity by injection of SUK-4 Ab caused a dramatic change in the pattern of substrate adhesions over the 3-h assay period. The change observed was similar to that induced by microtubule disassembly with nocodazole (Fig. 1), and characteristically involved a major lengthening and reduction in the number of adhesion sites (Fig. 2). Similar changes after SUK-4 antibody injection were observed in time-lapse videos of cells transfected with GFP-zyxin (Fig. 6, C and D; Video 3, available at http://www.jcb.org/cgi/content/full/jcb.200105051.DC1). Because zyxin also binds to stress fiber assemblies, the growth and lengthening of adhesions could be readily correlated with the growth of stress fiber bundles (Fig. 6, C and D).
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For cells transfected with GFP-tubulin, we could first show that the general distribution of microtubules in Xenopus fibroblasts was unaffected by injection of the SUK-4 antibody or 20 µM T93N kinesin (Fig. 8). At higher concentrations of T93N kinesin (100 µM) we observed bundling of microtubules, as was also seen in cells strongly expressing the GFP-tagged T93N construct (unpublished data); hence, the choice of the lower concentration for the kinesin inhibition experiments. Table I presents the results of measurements of microtubule dynamics for control and injected cells transfected with GFP-tubulin. As shown, injection with SUK-4 Ab or 20 µM T93N kinesin had no measurable influence on microtubule dynamics, the values for control and injected cells being comparable to those documented for other cell types (Shelden and Wadsworth, 1993).
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Discussion |
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Rodionov et al. (1993) previously showed that injection of a polyclonal antibody (HD antibody) raised against the motor portion of Drosophila conventional kinesin (kinesin-1) induced morphological changes in fibroblasts similar to those seen after microtubule disassembly: namely, a loss of asymmetry and a suppression of peripheral motility. They concluded that microtubules serve to deliver components to the cell periphery required for lamellipodia activity. Subsequent work from the same group demonstrated that cells injected with the HD antibody generally exhibited larger peripheral adhesion sites (marked by antibodies to vinculin) than observed in uninjected cells, and pointed to the similarity of this effect to that observed with nocodazole (Kaverina et al., 1997). Although these studies are consistent with the present findings, interpretation of results obtained with the HD antibody were complicated by this antibody's apparent inhibition of other members of the kinesin superfamily (Tuma et al., 1998; Kreitzer et al., 2000), and to its reported inhibition of microtubule dynamics (Waterman-Storer, C.M., personal communication).
With the SUK-4 antibody, which is monospecific for kinesin-1 heavy chain from diverse species (Ingold et al., 1988), kinesin motor activity was inhibited, as judged by the perinuclear accumulation of mitochondria, without any detectable effect on either microtubule dynamics or microtubule targeting of adhesion sites. Therefore, we conclude that the observed enlargement and elongation of adhesion sites seen after injection of the SUK-4 antibody was due to a block in the transport of a signaling complex linked to kinesin-1, and not to other effects arising from a modulation of microtubule dynamics (Hunter and Wordeman, 2000) or to the inability of microtubules, carrying for example a passively bound signal, to interact with focal adhesions. At appropriate concentrations, the kinesin heavy chain T93N rigor-like construct also blocked kinesin motor activity without affecting microtubule dynamics or the targeting of adhesion sites by microtubules. Because T93N kinesin can potentially block the binding of other kinesin motors to the microtubule lattice, it is likely less specific for inhibiting kinesin-1 than the SUK-4 antibody. Nevertheless, by this alternative route of kinesin inhibition we observed the same amplification of adhesion sites and cell depolarization as seen with the SUK-4 antibody, again implicating kinesin-1 in the transmission of modulators of adhesion site dynamics along microtubules.
Because targeting was unaffected by blocking kinesin motor activity, the guidance of microtubules to adhesion sites must occur via a kinesin-1independent mechanism, presumably involving cross-linkers (Gavin, 1997) or complexes such as between other kinesins and unconventional myosins (Huang et al., 1999) that can potentially couple microtubules to actin. Given that components of the dynactin complex are located at microtubule ends (Vaughan et al., 1999), and that one of them, p62, was partially localized to focal adhesions (Garces et al., 1999), we considered the possibility that targeting of microtubules to adhesion sites may require the dynein complex. However, dissociating the dynein/dynactin complex by the m742 antibody or dynamitin (Burkhardt et al., 1997; Steffen et al., 1997) had no effect on adhesion site dynamics, indicating that this complex is not directly involved in the modulation of substrate anchorage.
However, several other proteins have also been found concentrated toward the growing, plus ends of microtubules (Näthke et al., 1996; Mata and Nurse, 1997; Diamantopoulos et al., 1999; Browning et al., 2000; Brunner and Nurse, 2000; Mimori-Kiyosue et al., 2000a, 2000b). In fission yeast, three gene products required for polarized cell growth, Tea 1p, Tea 2p, and tip1p, localize to microtubule tips at the actin-rich ends of the cell, Tea 2p being a kinesin-like protein, and tip1p a CLIP170 homologue (Mata and Nurse, 1997; Browning et al., 2000; Brunner and Nurse, 2000). In yeast, microtubule stabilization is thought to be a primary factor in controlling and localizing cell growth, and both tip1p 1 and Tea 2p have been implicated in the control of microtubule dynamics (Browning et al., 2000; Brunner and Nurse, 2000). More recently, a new family of CLIP-associated proteins, CLASPS (Akhmanova et al., 2001), has been identified that associates with microtubules ends, but notably accumulates at the periphery of fibroblasts at sites reminiscent of focal adhesions (Akhmanova et al., 2001). This provocative localization, together with the growing evidence for the tethering of microtubule ends to cortical actin in other situations (Goode et al., 2000; Allan and Näthke, 2001), points to a likely role of microtubule tip complexes in adhesion site targeting.
The real possibility also exists that components specifically accumulated at microtubule tips act as cofactors, together with cargo delivered by kinesin, in signal transduction events. It has already been shown that both Rac (Best et al., 1996) and the Rho/Rac exchange factors, Lfc and p190RhoGEF, bind to microtubules (Ren et al., 1998; Glaven et al., 1999; van Horck et al., 2001) linking modulators of the actin cytoskeleton to the microtubule network. Rac activity can be stimulated by ASEF when this exchange factor is in a complex with the microtubule tipassociated protein APC (Kawasaki et al., 2000). Additionally pertinent to the present work are the observations that mixed lineage kinase and c-Jun kinase directly associate with members of the kinesin superfamily (Nagata et al., 1998; Verhey et al., 2001). These latter findings are pivotal in demonstrating the potential of kinesin to associate with and transport signaling complexes. Because kinesin-1 has been implicated in various trafficking activities (Hirokawa, 1998), its individual tasks are presumably specified through functionally tailored associations with different adaptor and signaling scaffolds (Burack and Shaw, 2000). The same scaffolds likely include regulators of motor activity (Nagata et al., 1998) to initiate and control cargo delivery. Our present studies now directly implicate kinesin-1 (KIF 5) (Hirokawa, 1998) as the carrier of components that signal the modulation of adhesion site turnover. The future challenge will be to identify the nature of this signaling cargo.
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Materials and methods |
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DNA
For expression of GFP-fused proteins, mouse ß tubulin in a pEGFP-C2 vector, human zyxin in a pEGFP-N1 vector, and the T93N mutant kinesin heavy chain cDNA, provided by N. Hirokawa (University of Tokyo, Tokyo, Japan) in EGFP-N3 vector were used. EGFP-zyxin and EGFP-tubulin were provided by J. Wehland and co-workers (GBF, Braunschweig, Germany).
Transfections
Subconfluent monolayer cultures on 30-mm Petri dishes were used for transfection. The transfection mixture was prepared as follows: 2 µg of DNA and 12 µl of Superfect lipofection agent (QIAGEN) were mixed in 300 µl of serum-free medium. After a 30-min incubation at room temperature, a further 1.2 ml of serum-free (for Xenopus fibroblasts) or 5% serum-containing medium (for CAR) was added. Cells were incubated in this mixture for 5 h and then returned to normal medium. After 24 h, cells were replated onto coverslips for microscopy.
Microinjection and proteins
Injections were performed with ready-made sterile Femptotips (Eppendorf) using a Leitz Micromanipulator with a pressure supply from an Eppendorf Microinjector 5242. Cells were injected with continuous outflow from the needle under a constant pressure of 2060 hPa.
Tetramethylrhodamin (5-Tamra; Molecular Probes) conjugated vinculin from turkey gizzard was kindly provided by M. Gimona and K. Rottner (Institute of Molecular Biology, Salzburg, Austria). Small aliquots in 2 mg sucrose/mg protein were stored at -70°C. Before use, the fluorescent vinculin was dialyzed against 2 mM Tris-acetate, pH 7.0, 50 mM KCl, 0.1 mM DTE and used at a concentration of 1 mg/ml. Cy3-conjugated tubulin was provided by J. Peloquin and G. Borisy (Northwesten University, Chicago, Illinois) It was stored at a concentration of 20 mg/ml in aliquots at -70°C. For coinjections; tubulin and vinculin were mixed in a proportion 1:3 after separate centrifugation for at least 15 min at 18,000 g and used immediately.
The purified monoclonal antibody against dynein intermediate chain (m742) has been described (Steffen et al., 1996, 1997). Monoclonal anti-kinesin antibody SUK-4 (Ingold et al., 1988), purified according to Tuma et al. (1998), was a gift of V. Gelfand (University of Illinois, Urbana-Champaign, IL). Both antibodies were stored at a concentration 10 or 4 mg/ml, respectively, in microinjection buffer aliquots at -70°C. Before injection, Abs were centrifuged for at least 10 min at 18,000 g. If needed, fluorescently labeled dextran was added to the injection solution as a marker.
Rigor kinesin
K340 T93N + 6His was constructed using PCR point mutagenesis: we separately amplified two fragments from the original full-length rat kinesin heavy chain clone using primers (M-55) 5'-CC GCT CTA CAT ATG GCG GAC CCA GCC GAATGC AGC-3' and (M-108) 5'-CTC CAT GGT ATG ATT TTT TCC TGA-3' for one reaction, and (M-109) 5'-TCA GGA AAA AAT CAT ACC ATG GAG-3' and (M-110) 5'-TCC CTC ATC GAT CAC ATC CAT GAC CTC CTC-3' for the other. The T93N mutation was thereby introduced via the 3' primer of one reaction and the 5' primer of the other. The forward primer M-55 introduced an Nde1 site at the 5' end of the product, and silently removed the original BamH1 site.
After amplification, the two PCR products were gel purified, mixed, denatured, annealed, and PCR amplified using primers M-55 and M-110, omitting the primers from the first five cycles of PCR. The gel-purified product was digested with NdeI and ClaI and ligated into Nde1- and Cla1-cut plasmid pETK340 + 6his (unpublished data), which fuses amino acids 1340 of kinesin HC to a COOH-terminal 6his tag.
Expression was performed in BL21(DE3) cells freshly transformed with p1020NdeI/NotIKinesin T93N ET + 6His plasmid. Overnight cultures of bacteria were diluted 1 in 50 into 2xYT medium supplemented with ampicillin at 100 µg/ml, and grown with shaking at 37°C until the absorbance at 600 nm was 1.0. The cells were shaken for a further 30 min at 22°C before induction with IPTG at 0.1 mg/ml, and after a further 5 h shaking at 22°C the bacteria were harvested by centrifugation. The cell pellets were frozen in liquid nitrogen and stored at -70°C.
Thawed cell pellets of 50 g were resuspended using a hand held Braun homogenizer in buffer A (20 mM Na2HPO4-NaH2PO4, pH 7.4, 2 mM MgCl2, 10 mM ME, 300 mM NaCl) at 1 g/3 ml, supplemented with complete protease inhibitor cocktail tablets EDTA-free (Roche) at the recommended dosage, and incubated on ice with lysozyme (0.1 mg/ml) and Triton X-100 (0.05%) for 15 min. The cells lysate was supplemented with MgCl2 to 10 mM and DNAase to 40 µg/ml and incubated for a further 15 min on ice. The supernatant was clarified by centrifugation 33,646 g at 4°C for 40 min, and the pellet was discarded.
This supernatant was mixed at the manufacturers recommended ratio with CoTalon superflow metal affinity resin (CLONTECH Laboratories, Inc.), preequilibrated in buffer A, and mixed in a roller bottle at 4°C for 30 min. The resin was sedimented at 2 K for 5 min, the supernatant discarded, and the beads loaded into a column. The column was washed until the flow through OD was zero using buffer W (20 mM Na2HPO4-NaH2PO4, pH 6.9, 2 mM MgCl2, 10 mM mercaptoethanol, 300 mM NaCl), and then further washed with the same buffer but with only 50 mM NaCl. Elution was performed used buffer E (20 mM Na2HPO4-NaH2PO4, pH 7.4, 2 mM MgCl2, 10 mM mercaptoethanol, 50 mM NaCl, 250 mM imidazole). Peak fractions were pooled, diluted one time with Q buffer (20 mM Na2HPO4-NaH2PO4 pH 7.0, 2 mM MgCl2, 10 mM mercaptoethanol), loaded onto a 1-ml HitrapQ cartridge, washed with Q buffer and then P buffer (20 mM Pipes pH 6.9, 2 mM MgCl2, 10 mM mercaptoethanol), and eluted in P buffer plus 100 mM NaCl. Peak fractions were pooled, supplemented with 20% glycerol and 100 µM ATP, aliquoted, and flash frozen in liquid nitrogen.
For microinjection, the construct was dialyzed at 4°C against 2 mM Tris-Acetate buffer, pH 7.0 (150 mM NaCl, 2 mM MgCl2, 100 µM ATP, 500 µM DTE), centrifuged for at least 15 min at 18,000 g, and used immediately.
Drugs
Nocodazole (Sigma-Aldrich) was added to culture medium from a 5 mg/ml stock solution in DMSO. Complete depolymerization of microtubules was achieved using a concentration of 2.5 µg/ml for 3 h. For the labeling of mitochondria, Rhodamine 123 (R-8004; Sigma-Aldrich) was added at a concentration of 10 µg/ml to the culture medium. Cells were incubated for 10 min and then returned to fresh medium. For the labeling of lysosomes, tetramethylrhodamine dextran (D-1816; Molecular Probes) at 0.05 mg/ml was added for 48 h to the culture medium.
Video microscopy
Cells were observed at room temperature on an inverted microscope (Axiovert 135 TV; Carl Zeiss) equipped for epifluorescence and phase-contrast microscopy, using 40X/NA 1.3 Plan-Neofluar or 100x/NA 1.4 Plan-Apochromat objectives and up to 1.6 optovar intermediate magnification. Data were acquired with a back-illuminated, cooled CCD camera from Princeton Research Instruments driven by IPLabs software (both from Visitron Systems) and stored as 16-bit digital images. Times between frames were 4 s (for assays of microtubule dynamics), 22 s (for observing targeting events), and 1 or 2 min (for observing longer-term changes in adhesion patterns). For some purpose, cells on engraved marker coverslips were injected with vinculin, followed with or without antibodies and images recorded directly after injection and 3 h later.
Quantitative analysis
For contact length measurements, equivalent regions in different cells were compared. Lengths of contacts were measured using IPLabs drawing tools, entered into worksheets (Sigma Plot v.4.1; SPSS, Inc.) and statistically analyzed. The dynamic excursion of microtubules was measured from sequences recorded with 4-s interval between frames using IPLabs segment tools. Microtubules that were clearly in focus for at least 30 frames were selected for tracking. All measurements were then entered into worksheets (Sigma Plot v.4.1; SPSS, Inc.). Phases of growth, shortening, pause, catastrophe, and rescue events were marked on plots and the times and rates calculated using Sigma Plot software.
Online supplemental material
The supplementary videos (available at http://www.jcb.org/cgi/content/full/200105051/DC1) show the dynamics of substrate adhesion site turnover and microtubule adhesion site interactions in either Xenopus or goldfish fibroblasts. Microtubules were fluorescently labeled by transfection of cells with EGFP-tubulin, or by microinjection of tubulin tagged with Cy-3. Adhesion sites were marked by transfection of cells with EGFP-zyxin or microinjection of cells with rhodamine (TAMRA)-vinculin. Additionally, the cells were manipulated to interfere with microtubule motor function, as described.
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Footnotes |
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* Abbreviation used in this paper: GFP, green fluorescent protein.
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Acknowledgments |
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This work was supported by a grant (P14007-Bio) from the Austrian Science Research Foundation.
Submitted: 9 May 2001
Revised: 4 December 2001
Accepted: 4 December 2001
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References |
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