Correspondence to Margaret Kielian: kielian{at}aecom.yu.edu
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Introduction |
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Alphaviruses and flaviviruses are small, spherical viruses containing plus-strand RNA genomes packaged with a capsid protein. The nucleocapsid is enveloped by a lipid bilayer containing the virus membrane fusion protein (alphavirus E1 or flavivirus E). This transmembrane (TM) protein mediates the fusion of the virus membrane with the cell membrane, delivering the viral RNA into the cytoplasm and initiating virus infection. In mature virions, alphavirus E1 is associated as a heterodimer with the viral E2 protein, whereas the flavivirus E protein is found as an EE homodimer. Infection by alphaviruses and flaviviruses occurs via an initial interaction of the virus with cell surface receptors, followed by internalization of the virus by endocytosis (for reviews see Kielian et al., 2000; Heinz and Allison, 2001). Virus membrane fusion is triggered by the mildly acidic pH within the endocytic pathway and is specifically blocked by inhibitors of endosome acidification. Low pH causes a dramatic rearrangement of the fusion protein, dissociating its dimeric interactions and producing a target membrane-inserted homotrimer (HT) that is believed to drive the membrane fusion reaction (Wahlberg and Garoff, 1992; Allison et al., 1995; Kielian et al., 1996).
Although the alphavirus and flavivirus fusion proteins do not have detectable amino acid sequence similarity, they have remarkably similar secondary and tertiary structures, indicating their evolutionary relationship and leading to their classification as the inaugural members of the class II virus fusion proteins (Lescar et al., 2001). The neutral pH structures of the fusion protein ectodomains have been determined for the alphavirus Semliki Forest virus (SFV; Lescar et al., 2001) and the flaviviruses TBE, DV2, and DV3 (Rey et al., 1995; Modis et al., 2003, 2005; Zhang et al., 2004). The proteins are elongated molecules composed almost entirely of ß strands and contain three domains: the centrally located domain I; domain II, which is located at one side of domain I and contains the target-membraneinteracting fusion peptide loop at its tip; and an Ig-like domain III, which is connected to the other side of domain I (Fig. 1 A). Although not present in the ectodomain structure, in the full-length proteins the stem region and TM anchor are found at the COOH terminus of domain III, at the opposite end of the protein from the fusion loop. The fusion proteins are arranged with icosahedral symmetry and lie tangential (almost parallel) to the virus membrane (Lescar et al., 2001; Kuhn et al., 2002; W. Zhang et al., 2002).
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Although the class I viral membrane fusion proteins are structurally very different from the class II proteins, the class I proteins are known to refold to a hairpin-like structure during fusion (for reviews see Skehel and Wiley, 2000; Jardetzky and Lamb, 2004; Earp et al., 2005). Class I proteins are exemplified by the influenza virus HA and the HIV-1 gp41. The class I proteins are trimeric both before and after fusion. In the postfusion conformation, the membrane-proximal COOH-terminal regions interact with a more NH2-terminal trimeric -helical coiledcoil domain to form a "trimer of hairpins" that brings the fusion peptides and TM domains together. Importantly, for several class I proteins, peptides containing sequences of these NH2- or COOH-terminal interacting regions can bind to the viral fusion protein and inhibit fusion and infection by preventing refolding to the final hairpin conformation (for review see Moore and Doms, 2003). This dominant-negative approach is exemplified by the HIV peptide T20/Enfuvirtide, a licensed antiretroviral drug that corresponds to the COOH-terminal helix of gp41 (Wild et al., 1993).
Inhibitors of the class II fusion proteins would be very valuable tools in studying fusion mechanisms and developing antiviral agents for these important viruses. The structures of the class II fusion proteins suggest several features that might serve as targets for inhibitors of the fusion reaction. The pH 7.0 form of the DV E protein reveals a hydrophobic pocket within a flexible "hinge" region between domains I and II (Modis et al., 2003). Because the hinge changes its angle during the transition to the trimer form, molecules that bind to the hydrophobic pocket may inhibit hinge flexibility and block fusion (Modis et al., 2003). The structure of the SFV E1*HT reveals that the stem region of the protein interacts along the "core trimer," which is the central region of the trimer containing domains I and II (Gibbons et al., 2004b). Thus, the stem peptide and its HT interaction site are potential targets (Bressanelli et al., 2004; Modis et al., 2004). One of the most prominent features of the class II protein refolding reaction is the striking movement of domain III and the stem toward the trimer tip (Fig. 1 A). Although domain III undergoes a dramatic reorientation during fusion, the significance of domain III in the fusion protein refolding reaction and in driving membrane fusion is unknown.
We demonstrate here that recombinant forms of domain III can inhibit the low pH-dependent fusion reactions of the alphaviruses and flaviviruses. Domain III proteins show cross-inhibition within each virus genus, suggesting the presence of critical interaction sites. Studies of SFV demonstrate that inhibition occurs by a dominant-negative mechanism in which exogenous domain III binds stably to an E1 trimer intermediate and blocks the initial mixing of the target and virus lipid bilayers. Our results suggest a new antiviral strategy that should be generally applicable to all class II viruses.
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Results |
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Inhibition of class II virus fusion and infection by domain III proteins
We screened the SFV DIII proteins for activity in a fusion-infection assay (FIA) that quantitates low pH-dependent SFV fusion with the plasma membrane (Vashishtha et al., 1998). Viruses were bound to cells on ice and treated for 1 min at 37°C at low pH to trigger the fusion of the virus with the plasma membrane of the cell. This fusion results in virus infection. The cells were cultured overnight in the presence of 20 mM NH4Cl to prevent secondary infection, and the cells infected due to the low pH pulse were quantitated by immunofluorescence. Under these conditions, we could test the effects of domain III proteins specifically during the binding step, the fusion step, and the postfusion culture step. As shown in Fig. 2 A, 4 µM His-DIII almost completely inhibited SFV infection of BHK cells, but only when present during the low pH-induced fusion step. Similar results were obtained for His-DIIIS (unpublished data). In contrast, preincubation of the virus with domain III proteins at 37°C at neutral pH had no effect (unpublished data). In agreement with studies showing that alphavirus receptor interaction is mediated by the E2 protein (for review see Schlesinger and Schlesinger, 2001), exogenous domain III proteins did not inhibit virus cell binding or release prebound virus from cells (Fig. 2 A and see Fig. 6). Inhibition by domain III protein was comparable when virus was prebound to cells at pH 6.5, 6.8, 7.4, or 8.0, or when the low pH pulse was at pH 5.5 or 6.0 (unpublished data). Comparison of the four SFV domain III proteins showed that the strongest inhibition was obtained with His-DIIIS (IC50 0.1 µM), followed by His-DIII (IC50
0.5 µM), DIIIS (IC50
6 µM), and DIII, which gave
40% inhibition at a concentration of 80 µM (Fig. 2 B). Thus, the presence of both the stem region and the NH2-terminal His tag resulted in increased effectiveness. Although enhancement by the stem region is suggested from the structure of the low pH-induced HT, the reason for the increase in inhibition observed with His-tagged forms of SFV domain III is not known. The tag at the domain III NH2 terminus could act by stabilizing binding to E1, mimicking the important domain Idomain III linker region and/or enhancing its trimeric interactions, concentrating the protein at the membrane at low pH, preventing displacement of the exogenous DIII by the endogenous DIII, and/or preventing cooperative HTHT interactions. High concentrations of His-tagged DV2 domain III protein did not affect SFV fusion (Fig. 3 B), indicating that there is no nonspecific effect of the His tag.
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To address the general applicability of domain III inhibition to class II fusion, we tested the ability of His-DV2DIII and DV2DIIIH1 to inhibit fusion by the DV2 and DV1 serotypes. These two serotypes show 60% overall sequence identity in domain III. Unlike alphaviruses, flavivirus receptor binding is directly mediated by the membrane fusion protein (Lindenbach and Rice, 2001). Prior studies of flavivirus domain III showed that it could block virus-cell binding (Bhardwaj et al., 2001; Hung et al., 2004). Therefore, we prebound DV1 and DV2 to cells in the cold and added domain III protein only during the 1-min pH pulse used to trigger fusion. As shown in Fig. 3 B, DV2DIIIH1 strongly inhibited both DV1 and DV2 fusion (
70% inhibition of DV2 at a concentration of 50 µM), but showed no activity against SFV. Interestingly, His-DV2DIII did not inhibit DV fusion, suggesting a possible role for helix 1, an NH2-terminal region of the stem previously shown to promote E protein trimerization (Allison et al., 1999). We have less information on the domain III requirements for optimal DV inhibition and have not yet evaluated if constructs containing both helix 1 and the NH2-terminal His tag would show increased activity. Treatment at 37°C for 1-min at neutral or low pH with DV2DIIIH1 did not release prebound virus from the cell membrane (Fig. 3 C), indicating that domain III inhibition was not due to effects on virus receptor interaction. Exogenous domain III can thus act as a specific inhibitor of the class II membrane fusion reaction. The observed cross-inhibition within the alphaviruses and flaviviruses suggests conservation of domain III contacts.
Because alphavirus receptor binding is not mediated by the E1 protein, we used this system to test the ability of domain III proteins to inhibit virus fusion from within the endosome, which is the physiological route of virus infection. We infected BHK cells with either SFV, SIN, vesicular stomatitis virus (VSV), or DV2, in the presence or absence of 20 µM His-DIIIS or 40 µM DIIIS. VSV, an unrelated rhabdovirus, and DV2 are important controls because these viruses also infect cells by endocytosis and low pH-triggered fusion (Matlin et al., 1982; Heinz and Allison, 2001). After a 1-h endocytic uptake period, NH4Cl was added to prevent further infection, and the primary infected cells were quantitated by immunofluorescence. Infection by both alphaviruses was significantly inhibited by the inclusion of either His-DIIIS or DIIIS (Fig. 4). In contrast, VSV and DV2 infection was not inhibited. Compared with the FIA, inhibition of alphavirus endocytic infection required a higher concentration of His-DIIIS and also showed lower efficacy versus untagged DIIIS. This could reflect relatively inefficient endocytic uptake of His-DIIIS by the cells or differential routing of virus and domain III within the endocytic pathway. Although its targeting to the endosomal site of virus fusion is probably not optimized, it is already clear that domain III protein can block fusion and infection under physiological virus entry conditions.
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Dominant-negative binding of exogenous domain III would be predicted to alter the conformation of the E1 HT by preventing the folding back of the viral domain III, and consequently could decrease trimer stability. Exposure of the acid-conformationspecific mAb E1a-1 epitope on domain I closely correlates with HT formation, although the epitope is not formed by trimerization per se (Ahn et al., 1999). Interestingly, concentrations of His-DIIIS above 2 µM led to a gradual decrease in the retrieval of E1 by both the anti-His antibody and mAb E1a-1 (Fig. 6 B). This suggests that the binding of exogenous domain III is directly affecting the conformation of the E1HT. Destabilization of the trimer structure by domain III could also explain why somewhat less trypsin-resistant trimer was recovered after retrieval with domain III, as compared with the acid-specific mAb (Fig. 6 C). We directly evaluated HT stability by following the resistance of the SFV HT to dissociation by SDS sample buffer at 30°C (Fig. 6 D). Increasing amounts of domain III proteins lead to the loss of the SDS-resistant HT conformation, with only 10% of the control HT observed in the presence of 10 µM His-DIIIS. Interestingly, bands migrating above and below the position of the E1HT were clearly observed with His-DIIIS, suggesting the presence of alternative E1 complexes. A decrease in the SDS-resistant E1HT was also observed in the presence of increasing amounts of His-DIII (60% of control HT at 10 µM His-DIII). Together, these results support a model (Fig. 7) in which exogenous domain III binds to an intermediate trimeric conformation of E1 and prevents final hairpin formation and fusion.
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Discussion |
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Our studies with SFV demonstrate that exogenous domain III stably interacted with a trimeric form of E1. Domain III inhibition thus identifies an important intermediate in the fusion reaction, defined by the presence of a relatively long-lived "target" core trimer (Fig. 7 C). The HT structure suggests that the binding site for exogenous domain III would be located within the groove formed by two E1 subunits in the central domain I/domain II portion of the class II hairpin (Gibbons et al., 2004b). This model agrees well with our finding that the monomer did not bind domain III because initial oligomerization would be required to form the critical binding site. The binding of exogenous domain III to the core trimer was very stable, resisting repeated detergent washes during coimmunoprecipitation. Binding affected the conformation of the HT to varying extents by preventing the normal folding back of one or more E1 subunits (Fig. 7). Inhibition showed cross reactivity among related viruses, in keeping with the presence of conserved residues in the domain IIIcore trimer interface. Although our data do not yet indicate which residues are most critical to the domain III interaction, they clearly suggest conservation of key protein contacts. The SFV E1HT structure indicates that the AB loop and C'-strand may be important in this interaction (Gibbons et al., 2004b). Together, the properties of inhibition indicate that domain IIIcore trimer binding is an important step in fusion protein refolding, with the potential to provide significant driving force in fusion.
Our studies also revealed a strong enhancement of inhibition by the presence of the fusion protein stem region. We have tested several stem peptides for their ability to inhibit SFV HT formation and/or membrane fusion (unpublished data). To date we have not observed inhibition by the stem region alone, and thus we hypothesize that domain III acts to orient the stem for its interaction with the core trimer (but see flavivirus results in Hrobowski et al., 2005). It may also be that the binding site for domain III is kinetically favored compared with that of the stem, which may occur later in the fusion reaction and be relatively short-lived. Our data are consistent with the exogenous domain IIIcore trimer interaction acting as a key first step in inhibition, with subsequent stem binding along the body of the trimer providing further stabilization of the inhibitory interaction.
An alternative model for inhibition by domain III is that it prevents cooperative HTHT interactions during fusion. Studies of the membrane insertion of class II fusion protein ectodomains indicate that insertion is highly cooperative (Gibbons et al., 2003; Stiasny et al., 2004). In the case of SFV, ectodomain insertion produces rings of five to six trimers, reflecting the physical associations of adjacent HTs through interactions of their fusion loops and of their domain III regions (Gibbons et al., 2003, 2004b). These cooperative interactions produce a volcano-like assembly of E1HTs that may help to induce membrane curvature at the fusion site (Gibbons et al., 2004b). Although we hypothesize that these intertrimer interactions are important for fusion, we feel that the strongest model for the action of domain III is that it acts not to prevent interactions between adjacent HTs, but to inhibit the foldback reaction within one E1 molecule. This agrees well with the resistance of domain IIIE1 binding to octylglucoside, a detergent that we previously found disrupted HTHT interactions (Gibbons et al., 2003, 2004a). It also agrees with the increase in inhibition and binding that is observed when the stem is present on domain III because no role for the stem in HTHT interactions was observed in the previous studies. However, domain III could also be acting by some combination of these two models. For example, prevention of E1 refolding by binding of exogenous domain III could inhibit the ability of the viral domain III to interact with an adjacent trimer.
Both the alphavirus and flavivirus fusion reactions are very rapidly triggered by low pH, with maximal fusion observed within seconds of low pH treatment at 37°C (Kielian et al., 2000; Heinz and Allison, 2001). Given the speed of the SFV fusion reaction, it is perhaps surprising that exogenous domain III can compete with the endogenous domain III for binding to the core HT. Such an intermolecular interaction of domain III would seem to be at a disadvantage compared with the intramolecular interaction of the viral domain III. Several factors may help to explain this paradox. The movement of domain III in the full-length E1 may be constrained by its attachment to the virus membrane through the stem/TM domains. Indeed, we found that binding of exogenous domain III to the E1* ectodomain trimer was not as efficient as binding to the full-length trimer (unpublished data), in keeping with the possibility of a more rapid foldback of E1* domain III due to the loss of its membrane anchor constraint. The structure of the E1 HT also reveals that the linker region between domain I and III becomes highly extended during the movement of domain III toward the fusion loop (Gibbons et al., 2004b). This could provide an additional constraint to viral domain III movement, favoring the interaction of exogenous domain III with the core trimer.
Previous studies of flavivirus domain III proteins have focused on their interactions with virus receptors on the cell surface. Our data identify a novel inhibitory effect of exogenous domain III in the fusion reactions of alphaviruses and flaviviruses. Such domain III proteins can serve as useful basic research tools to study alphavirus and flavivirus fusion. Although unlikely to be directly applicable as antivirals, their inhibitory activity has important implications for the development of clinically useful inhibitors of the class II fusion reaction. Because exogenous SFV domain III showed stable binding to a trimeric E1 target, this interaction could be used to screen for peptides or small molecules that would block critical domain IIItrimer contacts. Given the cross-inhibition we have observed with domain III, such screens have the potential to identify broad-specificity inhibitors of class II fusion proteins.
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Materials and methods |
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Construction of domain III protein expression plasmids
DNA sequences of SFV E1 domain III (with or without stem region) were amplified from DNA derived from the infectious SFV clone of Toto1101 (Chatterjee et al., 2002), and the DNA sequences of DV2 E domain III were obtained by RT-PCR using viral RNA extracted from DV2-infected C6/36 cells. These sequences were subcloned into the protein expression plasmid pET-14b (Novogen) to express domain III proteins with an added NH2-terminal methionine, or the pRSET A plasmid (Invitrogen) to express NH2-terminal 6x Histidine-tagged domain III proteins with an added NH2-terminal 36 amino acids, and confirmed by DNA sequencing.
Protein expression, refolding, and purification
Domain III proteins were expressed and refolded essentially as described for other Ig-like domain proteins (X. Zhang et al., 2002). In brief, proteins were expressed in E. coli strain BL21(DE3), solubilized from inclusion bodies in buffer containing 6 M guanidine-HCl, refolded by the fast dilution method, and purified by fast protein liquid chromatography on a Superdex G-75 gel filtration column (GE Healthcare). The concentration of purified domain III proteins was determined by absorption at 205 nm (Scopes, 1974).
Protein analysis
The mass of domain III proteins was measured by ESI mass spectrometry using a Finnigan LCQ ion trap mass spectrometer (Thermo Electron Corporation). Analysis by SDS-PAGE was performed using a standard Trisglycine system and 11% acrylamide gels, except in Fig. 1 C, where a Tristricine buffer system and 16.5% acrylamide gels were used (Gibbons and Kielian, 2002). 35S-labeled proteins were quantitated by PhosphorImager analysis with Image Quant version 1.2 software (Molecular Dynamics).
FIA
Fusion of viruses with the plasma membrane of BHK cells was assayed using a variation of a previously described SFV FIA (Vashishtha et al., 1998). BHK cells grown on 12-mm coverslips in 24-well plates were washed twice with ice cold binding medium (RPMI without bicarbonate, plus 0.2% BSA, 10 mM Hepes, and 20 mM NH4Cl) at the indicated pH. Cells were incubated on ice for 90 to 120 min with gentle shaking with SFV and SIN in binding medium plus 20 mM MES, pH 6.8, or with DVs in binding medium, pH 7.9. Cells were washed twice with binding medium to remove unbound virus and pulsed for 1 min at 37°C in 200 µl pH medium (RPMI/BSA/Hepes plus 30 mM MES for pH 7.4 or RPMI/BSA/Hepes plus 30 mM sodium succinate for pH 6.0 or lower). SFV- and SIN-infected cells were incubated at 28°C overnight in BHK growth medium plus 20 mM NH4Cl; DV-infected cells were incubated in MEM plus 2% FCS and 50 mM NH4Cl for 3 h at 37°C, and then at 37°C for 2 d in the presence of 20 mM NH4Cl. Domain III proteins were added at specific steps as indicated. The threshold for DV fusion (approximately pH 7.0) was higher than that of SFV, with maximal DV fusion observed at approximately pH 6.2 and below, resulting in infection by 30% of the bound infectious DV.
Immunofluorescence microscopy
VSV-infected cells were fixed in 3% formaldehyde at RT for 20 min and GFP-expressing cells were quantitated by fluorescence microscopy. Cells infected by all other viruses were fixed in ice-cold methanol for 10 min. SFV- and SIN-infected cells were stained with rabbit polyclonal antisera against SFV or SIN envelope proteins and fluorescein-conjugated secondary antibody (Vashishtha et al., 1998). DV2- and DV1-infected cells were stained with a mouse polyclonal hyperimmune ascitic fluid against DV2 (obtained from R.B. Tesh, University of Texas Medical Branch, Galveston, TX), followed by an Alexafluor 488conjugated secondary antibody (Molecular Probes). For each sample, duplicate coverslips were evaluated at an infection level of >200 positive cells/coverslip in the absence of inhibitor.
Assays of the SFV E1 HT
To assess the conformational change of SFV E1 protein during fusion in the presence of domain III proteins, purified 35S-labeled SFV was bound to BHK cells on ice and pulsed at low pH, as in the FIA. The cells were washed to remove exogenous domain III and solubilized in lysis buffer (20 mM Tris, pH 7.4, 100 mM NaCl, 1.5% octylglucoside, 1 mM EDTA, plus 1 µg/ml pepstatin, 50 µg/ml leupeptin, 0.1% BSA, 100 µg/ml aprotinin, and 1 mM PMSF). To quantitate the SDS-resistant E1 HT, an aliquot of each lysate was added to SDS sample buffer and heated to 30°C for 3 min before SDS-PAGE. Another aliquot of cell lysate was subjected to immunoprecipitation (Kielian et al., 1996) using the indicated antibodies and zysorbin as immunosorbant, and analyzed by SDS-PAGE.
To test trypsin resistance, the zysorbin with retrieved immunoprecipitate was pelleted, resuspended in PBS containing 1% Triton X-100, and digested with 125 µg/ml trypsin at 37°C for 1 h. The digestion was stopped by adding 5 mM PMSF. The zysorbin was eluted by treatment with 2% SDS and three cycles of heating to 95°C for 3 min. It was pelleted and the supernatants were concentrated by acid precipitation and analyzed by SDS-PAGE.
Pyrene-labeled SFV fusion with cells
Pyrene-labeled SFV was prepared by propagation of virus in BHK cells metabolically labeled with C16-pyrene (Chatterjee et al., 2002) and tested using a protocol similar to the FIA. Virus was bound on ice for 120 min to BHK cells on 35-mm plates at a multiplicity of 2,000 pfu/cell. Virus fusion was induced at 37°C for 1 min in pH 7.4 or 5.5 medium. Cells were put back on ice and washed once with binding medium at pH 6.8 and once with H-H solution (HBSS containing 10 mM Hepes, pH 7.4, and 20 mM NH4Cl). The cells were scraped in H-H solution, transferred to a quartz cuvette, and the emission spectrum determined using an AB-2 fluorometer (Thermo Electron Corporation) at 37°C with an excitation wavelength of 343 nm (Chatterjee et al., 2002). The fluorescence emission from 360 to 560 nm was recorded as the average of two serial scans, and the background fluorescence from cells alone was subtracted from each sample. The excimer and monomer peaks were determined at emission wavelengths of 475 and 397 nm, respectively.
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Acknowledgments |
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This work was supported by a grant to M. Kielian from the Public Health Service (R01 GM52929) and by a Cancer Center Core Support grant (National Institutes of Health/National Cancer Institute P30-CA13330).
Competing interests statement: M. Kielian and M. Liao have filed a provisional patent based on this study.
Submitted: 18 July 2005
Accepted: 2 September 2005
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