* Department of Cell Biology, and The Skaggs Institute of Chemical Biology, The Scripps Research Institute, La Jolla, California
92037; Department of Biological Science, The California State University, Fullerton, California 92834-6850; and § Department of
Biological Science, Lehigh University, Bethlehem, Pennsylvania 18105
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Abstract |
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A set of nuclear mutants of C. reinhardtii were identified that specifically lack translation of the chloroplast-encoded psbA mRNA, which encodes the photosystem II reaction center polypeptide D1. Two of these mutants are deficient in the 47-kD member (RB47) of the psbA RNA-binding complex, which has previously been identified both genetically and biochemically as a putative translational activator of the chloroplast psbA mRNA. RB47 is a member of the poly(A)-binding protein family, and binds with high affinity and specificity to the 5' untranslated region of the psbA mRNA. The results presented here confirm RB47's role as a message-specific translational activator in the chloroplast, and bring together genetic and biochemical data to form a cohesive model for light- activated translational regulation in the chloroplast.
Key words: genetic translation; initiation factors; translational regulation; gene expression ![]() |
Introduction |
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ANALYSIS of photosynthetic gene expression in the unicellular green algae Chlamydomonas reinhardtii
has shown that for chloroplast mRNAs, translational regulation is a prevalent mechanism used for controlling the synthesis of key photosynthetic proteins. Translation of a number of these chloroplast mRNAs is
strongly influenced by light, with high levels of protein
synthesis initiated from preexisting pools of mRNA only
after transfer of cells into light. Genetic analysis has identified a number of nuclear mutants in which translation of
specific chloroplast mRNAs is lacking. In many of these
mutants, translation of a single mRNA was affected, whereas other photosynthetic mRNAs were translated at
normal levels (Kuchka et al., 1988; Rochaix et al., 1989
;
Girard-Bascou et al., 1992
; Yohn et al., 1996
). Mutants affecting mRNA processing (Goldschmidt-Clermont et al.,
1990
) and mRNA stability (Kuchka et al., 1989
) have also been identified, suggesting that these processes also play
roles in gene expression within the chloroplast. In addition, protein turnover has been shown to affect accumulation of photosynthetic proteins in the chloroplast (Erickson et al., 1986
; Mayfield et al., 1987
). Though each of
these processes plays some role in regulating protein accumulation, translational regulation appears to be the predominant form of light-mediated gene regulation in the
chloroplast (for review see Rochaix, 1992
; Gillham et al., 1994
; Mayfield et al., 1995
).
Analysis of nuclear mutants that affected translation of
specific chloroplast mRNAs suggested that nuclear factors
act as translational activators. These translational activators interact with the 5' untranslated region (UTR)1 of
chloroplast-encoded mRNAs to facilitate translation of
the downstream coding region (Mayfield et al., 1995). This
RNA-protein interaction was first suggested by chloroplast mutations that were localized to the 5' UTR of the
mRNA and that affected translation of the downstream
encoded message (Rochaix et al., 1989
). A chloroplast suppressor was also identified that restored translation to
a nuclear mutant lacking translation of the chloroplast
psbC mRNA (which encodes P6, a component of photosystem II [PS II]). This suppressor mutation was localized
in the 5' UTR of the psbC mRNA (Rochaix et al., 1989
).
The 5' UTR of the psbA mRNA (encoding D1, a PSII
subunit) of tobacco was shown to confer light-regulated translation on a reporter coding region in vivo (Staub and
Maliga, 1993
). Site-directed mutagenesis to the 5' UTR of
psbA mRNA of C. reinhardtii has further identified RNA
elements critical for translation of this mRNA. A consensus Shine-Dalgarno sequence located 26 nucleotides 5' of
the initiation codon was required for mRNA/ribosome association and translation, whereas changes to a predicted
stem-loop structure located adjacent to this potential ribosome binding sequence dramatically reduced psbA translation in the light (Mayfield et al., 1994
).
RNA-binding proteins, which were originally identified
based on in vitro binding that correlated with translation
rates under a variety of conditions, are part of a complex
that binds to the 5' UTR of the psbA mRNA with high affinity and specificity (Danon and Mayfield, 1991). The biochemical identification of these putative translational activators of psbA, as well as elucidation of mechanisms that
control psbA RNA-binding activity (Danon and Mayfield,
1994a
,b), suggested that multiple components are required
for light-activated translation of this specific chloroplast-encoded message. Three of the components of this complex, the 38-, 47-, and 60-kD proteins, have been cloned
and sequenced. This characterization identified the 47-kD
protein (RB47) as a member of the poly(A)-binding protein family (PABP) (Yohn et al., 1998
), the 60-kD protein
(RB60) as a protein disulfide isomerase (PDI) (Kim and
Mayfield, 1997
), and the 38-kD protein as a novel protein
with no characterized homologue identified in other species. These proteins bind as a complex to the 5' UTR of
the psbA mRNA, along with uncharacterized protein of
55 kD. The light dependence of this RNA binding was shown to be mediated by redox potential (Danon and
Mayfield, 1994b
), specifically through the 60-kD PDI
modulating redox status of RB47 (Kim and Mayfield,
1997
).
Characterization of C. reinhardtii nuclear mutants deficient in psbA translation has shown that members of the
psbA RNA-binding complex are affected in these mutants.
The nuclear mutant F35 was deficient in translation initiation of the psbA mRNA, implying that initiation may be
the stage at which translation is regulated by the translational activator proteins (Girard-Bascou et al., 1992; Yohn
et al., 1996
). Both the 47- and 55-kD psbA RNA-binding proteins accumulated at reduced levels in this mutant, suggesting that a reduction in accumulation of these two putative translational activators might have caused the loss of
translation of psbA mRNA in this strain (Yohn et al.,
1996
). The nuclear mutant nac1-18 lacks translation of
both psbA and psbD mRNAs (Cohen, A., C.B. Yohn, and
S.P. Mayfield, manuscript submitted for publication). This
mutant was shown to be blocked in translation elongation of the psbD mRNA. This translation arrest led to accumulation of aberrant forms of the RB47 protein (Cohen et al.,
manuscript submitted for publication), subsequently resulting in a reduction of psbA translation initiation.
The data gathered to date suggest a role for RB47 and
the psbA RNA-binding complex in translation initiation of
the psbA mRNA. To further identify and characterize proteins required for psbA translation, a library of mutants
was created by random DNA insertion mutagenesis (Tam
and Lefebvre, 1993), and screened for defects in translation of the chloroplast-encoded psbA mRNA. We have
characterized five nuclear mutants of C. reinhardtii that
specifically lack translation of the psbA mRNA. One of
these mutants, hf149, completely lacks the 47-kD psbA
RNA-binding protein (RB47) and any detectable psbA
mRNA-binding activity. Another mutant, hf261, contains
only 10% of the wild-type (wt) levels of this protein and
~10% of the psbA mRNA-binding activity. In both of
these mutants, the psbA mRNA fails to associate with ribosomes. Other chloroplast mRNAs associate with polyribosomes and are translated at normal levels in these
strains. The three additional nonallelic mutations show related phenotypes in terms of reduced mRNA translation
and reduced ribosome association of the psbA mRNA.
However, all three of these mutants have psbA RNA-binding activity and accumulate RB47 protein. The data
shown here provide direct evidence that the psbA RNA-binding complex is required for psbA mRNA translational
activation, and identifies these proteins, and the PABP homologue RB47 in particular, as translational activators of
the psbA mRNA.
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Materials and Methods |
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Cell Growth Conditions
C. reinhardtii strains were grown in complete media (Tris-acetate-phosphate [TAP]) (Harris, 1989) to a density of 5 × 106 cells/ml under constant
light. Cells were harvested by centrifugation at 4°C for 5 min at 4,000 g.
Cells were either used immediately or frozen in liquid N2 for storage at
70°C.
Mutagenesis and Screening
The C. reinhardtii strain Arg7/cw15, an arginine auxotroph and cell wall-deficient mutant, was grown in TAP media with 50 mg/liter L-arginine to a
density of ~106 cells/ml under constant light. Cells were washed with TAP
without L-arginine and resuspended at ~5 × 107 cells/ml in TAP. For each
glass bead transformation (Kindle, 1990), 400 ml of cells were placed in a
15-ml sterile conical tube (Falcon Plastics, Cockeysville, MD) with 300 ml
of sterile, acid-washed glass beads (0.5-mm average diam). 2 mg of linearized DNA was added and polyethylene glycol (molecular weight of 8,000 D) was
added to 5% (wt/vol) final concentration. The DNA was a 7.8-kb genomic
fragment containing the argininosuccinate lyase gene of C. reinhardtii
(Debuchy et al., 1989
) cloned into pBlueScript, and then linearized with
XbaI in the polylinker. The cells, with beads DNA and PEG, were vortexed at full speed for 10 s. After allowing the beads to settle, cells were
pipetted from the tube and spread on TAP media plates (with 1.5% agar).
Transformant colonies were allowed to grow in dim light for 10-14 d.
Transformants with a high fluorescence (hf) phenotype were identified
with a video system based on a setup from Bennoun and Delepelaire
(1982). Transformants were illuminated with a 300-W quartz-iodine lamp
filtered by a blue glass filter (model 51710, peak transmittance 480 nm;
Oriel Corp., Stratford, CT). A charge-coupled device camera (model XC-77; Sony Corp., Park Ridge, NJ) with a red filter (Wratten gelatin filter
89B, peak transmittance 720 nm; Eastman-Kodak Co., Rochester, NY) attached to the lens was used to visualize chlorophyll fluorescence in the
transformant colonies. The video signal was input into a Macintosh computer running the public domain NIH Image program (developed at the U.S. National Institutes of Health and available on the Internet at http://rsb.info.nih.gov/nih-image/) via a framegrabber (model LG-3; Scion
Corp., Frederick, MD). Integrating 4-8 frames allowed for easy visualization and selection of hf mutants.
Membrane and Soluble Protein Isolation and Immunoblotting
Protein isolation, electrophoresis, and immunoblotting were performed as
described by Mayfield et al. (1994), with the following modifications. After extraction, membrane or soluble proteins were separated by electrophoresis and electroblotted to nitrocellulose (Schleicher & Schuell,
Keene, NH) in 10 mM cyclohexylamino-1-propanesulfonic acid (CAPS),
pH 11, with 10% methanol. Rabbit polyclonal antisera specific for D1, D2,
ATPase, or LHC II proteins were added to blots of membrane proteins,
whereas antisera specific for OEE1 or the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase were used with blots of soluble proteins.
Pulse Labeling of Proteins with [14C]Acetate
Cells were labeled with [14C]acetate for 10 min in the presence of cycloheximide as described by Mayfield et al. (1994). Membrane proteins were
isolated, separated by size on SDS-PAGE, and then visualized by fluorography as described by Malnoë et al. (1988)
.
Genetic Analysis
Standard protocols were used for crosses, tetrad dissection, and the scoring of the phenotypes of meiotic progeny (Levine and Ebersold, 1960). In
most cases, tetrads resulting from backcrosses and intercrosses were incomplete, with fewer than four viable progeny. For one cross (hf859 × F35), random spore analysis was used to generate a larger number of meiotic progeny. In this case, the mating was done according to standard protocols, but zygotes were subsequently collected, transferred to 10 ml of
TAP liquid medium supplemented with arginine, and then allowed to germinate for 14-16 h in bright light. After germination, tetrads were collected by centrifugation at 2,500 rpm for 10 min, resuspended in 0.5 ml
TAP liquid medium supplemented with arginine, vortexed briefly to disperse meiotic progeny cells, and then plated onto TAP plates supplemented with arginine. Colonies were visible in 7-10 d.
Chromatography
Approximately 5 × 109 cells were resuspended in low salt extraction
buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 10 mM MgCl2, 5 mM -mercaptoethanol). After passage through a cell disruption bomb (Parr Instrument Co., Moline, IL), the soluble cell extract was applied to a 5-ml
Econo-Pac heparin cartridge (Bio-Rad Laboratories, Inc., Hercules, CA)
as described in Danon and Mayfield (1991)
. Partial purification of proteins over heparin agarose was used to reduce RNase activity and enrich
for mRNA-binding proteins.
T1-Gel Mobility Shift
Gel mobility shift (GMS) coupled with T1 RNase protection assays were
performed with heparin agarose fractions as described in Danon and Mayfield (1991). Equal quantities of protein lysate were incubated with 0.065 pmol 32P-labeled transcript, 20 mg tRNA, and 1 mg total RNA from the C.
reinhardtii strain Fud7 (a chloroplast mutant with a psbA gene deletion)
(Bennoun et al., 1986
). After 15 min at room temperature, 0.5 U of T1
RNase (Pharmacia Biotech., Inc., Piscataway, NJ) was added, and then
the reaction was incubated for an additional 5 min at room temperature
before electrophoresis of the sample on a 1× TBE, 5% native polyacrylamide gel. RNA-protein complexes were detected by autoradiography.
Protein Electrophoresis and Immunoblotting of HA Proteins
Equal quantities of heparin agarose fractions were mixed with 2× sample
buffer (5% SDS, 5% -mercaptoethanol, 400 mM Tris-HCl, pH 6.8, 10%
sucrose), heated to 60°C for 10 min, and then separated by size using SDS-PAGE. The gel was either stained with Coomassie blue in 40% methanol,
10% acetic acid or electroblotted to nitrocellulose as described above.
Blotted nitrocellulose filters were treated as described above, except polyclonal antisera specific for the RB38, RB47, or RB60 protein (Danon and
Mayfield, 1991
) was used and the incubation continued at 4°C for 16 h. Goat
anti-rabbit IgG alkaline phosphatase conjugate (Bio-Rad Laboratories.
Inc.) was used as the secondary antibody to visualize RB38, RB47, or RB60.
RNA/Polysome Isolation and Analyses
Preparation of RNA and fractionation of polysomes were performed as
described by Barkan (1988) with modifications as described in Yohn et al.
(1996)
.
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Results |
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Generation and Selection of PS II Mutants of C. reinhardtii
A library of nuclear mutants was generated by insertional
DNA mutagenesis using the arg7 gene to complement the
arg7 nuclear mutant of C. reinhardtii (Purton and Rochaix,
1995). Transformants were screened for potential PS II-
deficient mutations by chlorophyll fluorescence, immunoblot, and Northern analyses (refer to Materials and Methods and see Table I). Five mutants were identified (hf149, hf233, hf261, hf859, and hf1085) which failed to accumulate the D1 protein but contained wild-type amounts of
other chloroplast proteins. Preparations of membrane proteins were analyzed for the presence of D1, D2, ATPase,
the light-harvesting complex (LHC), and photosystem I. Soluble protein fractions were analyzed for the presence
of ribulose bis-phosphate oxygenase/carboxylase and a
component of the PS II oxygen evolution complex
(OEE1). As expected, none of the mutants that lacked accumulation of D1 had detectable accumulation of the D2
protein (Table I). Other chloroplast localized proteins accumulate at near wild-type levels in each of the mutant strains (Table I). A representative Western showing each
of the proteins analyzed in the hf149 strain is shown in Fig. 1.
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To determine if these mutants have a specific defect in psbA translation, the mutant strains were pulse labeled for 10 min with [14C]acetate to assay protein synthesis rates. Cycloheximide was added to inhibit cytoplasmic, but not chloroplast, translation. Fig. 2 shows the relative levels of expression of the predominant chloroplast-encoded proteins in the hf149 mutant. This strain has undetectable levels of psbA translation with near wt translation of other chloroplast encoded mRNAs. The other four mutants had similar profiles (data not shown). Slight changes in the pattern of other chloroplast proteins were observed in the mutants compared with wt, these changes are likely due to degradation of other PS II proteins (e.g., P5 or P6), which turn over more rapidly in the absence of D1 accumulation.
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Genetic Analysis of Mutants
Intercrosses between mutant strains showed that mutants
hf261, hf859, and hf1085 fall into different complementation groups (Table II). Additionally, all three of these mutants identify genes that are distinct from the gene mutated in F35, another nuclear mutant affected in
translation initiation of the psbA mRNA (Yohn et al.,
1996). Strains hf149 and hf233 were not included in this
analysis because these strains failed to mate with the other
mutants, or produced too few viable progeny for a reliable analysis. These data show that at least four nuclear loci are specifically required for the expression of the psbA
mRNA in the chloroplast.
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Accumulation and Polysomal Association of psbA mRNA in Mutants
The absence of D1 protein synthesis in the nuclear mutants could be due to a variety of blocks in psbA gene expression. To determine if the psbA gene is transcribed and if psbA mRNA accumulates in the mutant strains, Northern blot analysis of psbA mRNA, encoding the D1 protein, was undertaken. As shown in Table I and Fig. 3, psbA and other chloroplast mRNAs accumulate in each of the mutants, although the psbA mRNA is at a somewhat reduced level relative to wt. The fact that measurable amounts of psbA mRNA accumulate, whereas no D1 protein synthesis is observed, suggests that translation is the defective step for D1 protein accumulation in each of these mutants.
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To identify the point at which translation is blocked, RNA/ribosome association was assayed for each of the mutant strains. This analysis should determine if the mRNA is able to associate with ribosomes and suggest whether the mutations affect translation at initiation or in events after initiation (i.e., elongation or termination). Polyribosomes were separated from mRNA not associated with ribosomes by pelleting through a sucrose cushion. psbA mRNA associated with polysomes and from whole cells was assayed in the hf149 mutant by hybridization of a psbA-specific probe. Reduced levels of psbA mRNA are associated with polysomal RNA relative to wt (Fig. 3 A), suggesting that the hf149 mutant is affected in its ability to initiate translation of the psbA mRNA. psbD mRNA shows essentially wt accumulation and polysome association in hf149 (Fig. 3 B), indicating that the inability of mRNA to associate with ribosomes in the hf149 strain is specific to the psbA mRNA. Mutants hf261 and hf859 also show greatly reduced psbA/ribosome association, whereas hf1085 has slightly reduced psbA polysome association (Fig. 3 C). Mutant hf233 appears to have fairly normal levels of psbA mRNA associated with polysomes. Each of these mutants have normal levels of psbD mRNA associated with ribosomes as expected (data not shown).
Status of psbA RNA-binding Complex and Accumulation of psbA RNA-binding Proteins
Binding of proteins to the 5' UTR of the psbA mRNA has
been shown to correlate with the level of D1 synthesis under varying physiological (Danon and Mayfield, 1991) and
biochemical (Danon and Mayfield, 1994a
,b) conditions,
and in different genetic backgrounds (Yohn et al., 1996
).
The psbA RNA-binding activity present in lysates from
these translation deficient mutants was analyzed by GMS
coupled with RNase T1 protection (Fig. 4, T1-GMS). In all cases, in vitro binding to the 5' UTR of psbA RNA is less
than that observed in wt lysates. Mutant hf149 shows no
detectable binding to psbA mRNA in vitro (Fig. 4),
whereas mutant hf261 shows binding activity at <10% of
wt levels. Mutants hf859 and hf1085 both have ~50% of
the binding activity of wt, whereas mutant hf233 has close
to wt levels of psbA RNA binding, although this mutant contains an additional RNA-protein complex that is not
seen in the wt or other mutant samples (Fig. 4).
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The in vitro binding described above is primarily due to
the binding of RB47 of the RNA-binding complex (Yohn
et al., 1998). This protein is a member of the PABP family
and is in close contact with the psbA mRNA (Danon and
Mayfield, 1991
). Immunoblot analysis was performed using antisera against RB47, as well as antisera against the
RB38 and RB60 components of the complex. Heparin
agarose-purified proteins from wt and each of the mutants
was assayed. This assay should indicate the amount of
these proteins in wt and mutant cells independent of their
ability to bind the psbA RNA, since binding of psbA
RNA-binding proteins to heparin agarose is unrelated to
their ability to bind psbA RNA (Yohn, C.B., and S.P.
Mayfield, unpublished data). Both the RB38 and RB60
proteins are present at levels similar to wt in all mutants
assayed (Fig. 5). When equal amounts of protein from wt
and hf149 are analyzed, no RB47 is found to accumulate in
hf149 cells (Fig. 6, middle panel), and RB47 cannot be detected even when a threefold excess of protein (the upper
limit for total protein loading in this system) is used. RB47
accumulation in hf261 was found to be <10% of wt levels (Fig. 6, right panel), whereas RB47 accumulation in the
other three mutants was found to be at least 50% of wt
levels. Accumulation of RB47 appears to correlate closely
to the level of in vitro binding detected by T1-GMS (refer
to Fig. 4). The loss of psbA binding activity in hf149 and
hf261 (and reduction in other mutants) as determined by
T1-GMS could therefore be accounted for by the loss of
the RB47 protein in these strains. Note that the hf233 strain, which contains an additional band in the RNA-binding assay, has an increased accumulation of a lower
molecular weight species of the RB47 protein (Fig. 6, right
panel).
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Discussion |
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Message-specific RNA-binding proteins have previously
been implicated in light-regulated translational activation
of the chloroplast encoded psbA mRNA (Danon and
Mayfield, 1991, 1994a
,b, Yohn et al., 1996
). The role of
one of these RNA-binding proteins, RB47, is now clearer,
since evidence for its function comes from several independent sources. First, biochemical analysis has shown
that this protein (along with other members of the psbA
RNA-binding complex) bind with high affinity and specificity to the psbA 5' UTR in a manner consistent with a
role in translational regulation; high levels of binding are
observed in the light when psbA translation is high, and
low levels of binding are observed in the dark when translation is low (Danon and Mayfield, 1991
). Second, the predicted amino acid sequence of RB47 suggests the role this
protein plays in translation. RB47 belongs to a family of
proteins known as poly(A)-binding proteins that bind
RNA and have been shown to play a role in translation
initiation (Sachs and Davis, 1989
; Proweller and Butler,
1996
; Tarun and Sachs, 1996
; Yohn et al., 1998
). Finally,
genetic analysis has predicted translational activators of
chloroplast mRNAs (Kuchka et al., 1988
; Rochaix et al.,
1989
; Girard-Bascou et al., 1992
; Yohn et al., 1996
), and
here we show by characterization of psbA translation deficient mutants that the absence of RB47 corresponds directly to the loss of translational initiation of the psbA
mRNA, thus defining RB47 as a translational activator of
the psbA mRNA.
Although proteins that bind to the 5' UTR of chloroplast mRNAs seem likely candidates for translational activators, no direct link had been made to the body of genetic data before the characterization of these mutants. The hf261 mutant is not likely to be a mutation directly in the RB47 gene, as this mutant is tagged with the arg7 gene and Southern and Northern analysis indicates that the RB47 gene is intact and produces normal amounts of RB47 mRNA in this strain (data not shown). The hf149 mutation is not tagged by the arg7 gene, so this mutation could potentially contain point mutations that disrupt RB47 accumulation while leaving the RB47 gene and RB47 mRNA accumulation intact (data not shown). These genetic data leave open the possibility that the absence of RB47 protein in these strains is the result of a loss of psbA translation, rather than the cause of it. Although this is a formal possibility, it is highly unlikely given the fact that the RB47 protein accumulates in the other psbA translation-deficient mutants described here (hf233, hf859, and hf1085), and that the other psbA RNA-binding proteins RB38 and RB60 accumulate to normal levels in all of the mutant strains. Thus, the hf149 and hf261 mutations provide strong evidence that the RB47 protein is directly involved in translational regulation of the chloroplast-encoded psbA mRNA. Other nuclear mutants determined to affect both RB47 and psbA translation further suggests the importance of this protein in psbA expression (Yohn, et al., 1996; Cohen et al., manuscript submitted for publication). Identification of the specific defect in the hf149 and hf261 mutant, as well as characterization of the other described mutants, should yield further insights into the process of psbA mRNA binding and the mechanism of translational regulation.
The dramatic reduction in the amount of psbA mRNA
associated with ribosomes in both the hf149 and hf261 mutants suggests that RB47 is specifically required for ribosomes to initiate translation of the psbA mRNA. Although
the identification of message-specific translational activators in the chloroplast has not previously been shown,
other organellar systems have been characterized in which
similar mechanisms for controlling and coordinating gene expression may be used, most notably the mitochondria of
yeast. The COX3 mRNA of Saccharomyces cerevisiae mitochondria is translationally regulated by a complex of at
least three proteins which have been shown genetically
(Wiesenberger et al., 1995) and biochemically (Brown et al.,
1994
) to interact with each other and with the COX3
mRNA. One of these proteins (PET122) also interacts
with the mitochondrial ribosome (Haffter et al., 1990
,
1991
; McMullin et al., 1990
), suggesting a model for translational activation in which these proteins facilitate the initial interaction between the mRNA and the ribosome. A
similar mechanism may be involved with RB47, the psbA
mRNA, and chloroplast ribosomes.
The involvement of RB47 in translational initiation of
psbA mRNA in the chloroplast is now clear, though the
exact role of this PABP is yet to be elucidated. At least
four genetic loci are implicated in RB47 accumulation and
psbA translation initiation. Some of these genes may encode proteins, other than RB47, that bind to the psbA
mRNA and are required for translation initiation of this
mRNA. The hf859 and hf1085 mutants may be this type,
since these mutations affect the ability of the psbA mRNA
to associate with ribosomes, but not to the same degree as
the hf149 and hf261 mutants. The psbA mRNA is associated with ribosomes in the hf233 mutant, so this mutation
appears to be acting after initiation and thus probably
downstream of the other mutations. The hf149 and hf261
mutants appear to act through RB47, either as mutations in the RB47 gene itself, or by specifically affecting accumulation of the RB47 protein. Thus the accumulation of
the RB47 protein might also be a regulated event. In fact,
cytoplasmic PABPs from other organisms have been
shown to be translationally regulated (de Melo Neto et al.,
1995; Bag and Wu, 1996). RB47 appears to share this characteristic in that the 5' UTR of the RB47 mRNA affects its
expression in heterologous systems (Mayfield, S.P., unpublished data). This regulated expression of RB47 may
be a mechanism for developmental regulation of photosystem accumulation in chloroplasts.
A model for the temporal regulation of psbA expression
in the chloroplast is described in Fig. 7. In this model, light
acts through PS II and PS I to produce a reducing environment in the chloroplast. Reducing equivalents are passed
to the chloroplast PDI (RB60) through ferrodoxin (FD)
and ferrodoxin-thioredoxin reductase. RB47's binding activity is then modulated by RB60 in a redox dependent
manner through reversible oxidation of sulfhydryl groups
within RB47 (Kim and Mayfield, 1997). Binding of RB47
to the psbA mRNA is required for RNA/ribosome association, where this protein acts as a message-specific translation initiation factor, bringing the mRNA and ribosome
together. Recently RB47 was shown to be associated with
the chloroplast membrane system (Zerges and Rochaix,
1998
), suggesting that RB47, along with other members of
the psbA RNA-binding complex, could be acting in both translational activation and as a targeting mechanism. Under this type of mechanism, the soluble RB47 protein
would bind the psbA mRNA and then direct this mRNA
to ribosomes specifically associated with the thylakoid
membranes, the site at which the hydrophobic D1 protein
is synthesized.
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Footnotes |
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C.B. Yohn's present address is Program in Developmental Genetics, The Skirball Institute, New York University Medical Center, 540 First Avenue, 4th Floor, New York, NY 10016.
Received for publication 11 May 1998 and in revised form 10 June 1998.
Address all correspondence to Stephen P. Mayfield, Department of Cell Biology, and The Skaggs Institute of Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037. Tel.: (619) 784-9848. Fax: (619) 784-9840. E-mail: mayfield{at}scripps.eduWe thank J. Allen (The Scripps Research Institute, La Jolla, CA) and J. Akman (California State University, Fullerton, CA) for technical assistance and D. Chagnovich (Skirball Institute, New York) and D. Sommers (The Scripps Research Institute) for critical reading of the manuscript.
This work was supported by funds from the National Science Foundation (MCB-9514698) to S.P. Mayfield, and the U.S. Department of Energy (90ER20009) and U.S. Department of Agriculture (93-37301-9428) to M.R. K Kuchka. A. Cohen was supported by a California State University Research, Scholarship, and Creative Activity Grant (1997-1998). C.B. Yohn was supported by an NSF predoctoral fellowship and an NSF graduate research traineeship.
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Abbreviations used in this paper |
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FD, ferrodoxin; hf, high fluorescence; LHC, light-harvesting complex; OEE1, oxygen evolution complex; PABP, poly(A)-binding protein; PDI, protein disulfide isomerase; PS II, photosystem II; T1-GMS, T1 RNase protection-gel mobility shift; TAP, Tris-acetate-phosphate; UTR, untranslated region; wt, wild type.
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References |
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