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Article |
Address correspondence to David B. Parkinson, Department of Anatomy and Developmental Biology, University College London, Gower Street, London, WC1E 6BT UK. Tel.: 44-20-7679-3365. Fax: 44-20-7679-2091. email: david.parkinson{at}ucl.ac.uk
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Abstract |
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Key Words: egr2; JIP-1; neuregulin; PNS; myelin
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Introduction |
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There is a strong temporal correlation between the onset of myelination and the cessation of cell division, and analysis with differentiation markers shows that although individual cells still proliferate in the earliest stages of myelin differentiation, they fall out of division before they start forming myelin sheaths, as detected by elevation in expression of the myelin protein Po (Friede and Samorajski, 1968; Brown and Asbury, 1981; Stewart et al., 1993). Similarly, apoptotic Schwann cell death declines in postnatal nerves as myelination advances and apoptosis is largely restricted to nonmyelinating cells (Grinspan et al., 1996; Syroid et al., 1996; Nakao et al., 1997). In line with this, TGFß, a factor implicated as a death signal in developing nerves, selectively induces apoptosis in nonmyelinating cells, and spares cells expressing myelin proteins (Parkinson et al., 2001).
Thus, Schwann cell myelination involves, first, escape from the cell cycle and developmental death signaling, followed by strong up-regulation of myelin proteins and formation of compacted membrane wraps.
The transcription factor Krox-20 is essential for myelination. It is strongly up-regulated by axon associated signals only in cells destined to myelinate and, in Krox-20 null mice, although myelin differentiation starts (Parkinson et al., 2003), myelin sheaths do not form and Schwann cells continue to proliferate and remain susceptible to death (Topilko et al., 1994; Zorick et al., 1999). In humans, Krox-20 (Egr2) mutations are associated with Charcot-Marie-Tooth, Dejerine-Sottas, and hereditary sensory and motor neuropathies, underlining the pivotal role of this protein in myelin formation (Wrabetz et al., 2001).
Here, we define the function of Krox-20 in Schwann cell proliferation and death, and analyze the molecular signaling that enables Krox-20 to control these events. We show that expression of Krox-20 is sufficient to cell autonomously alter the response of Schwann cells to the major axonal mitogen, ß-neuregulin-1 (NRG-1), so that NRG-1 no longer stimulates DNA synthesis. Similarly, Krox-20 inactivates TGFß death signals and protects cells from death triggered by growth factor deprivation. Notably, Krox-20 also blocks proliferation and death in 3T3 fibroblasts, although these cells are unrelated to Schwann cells. Moreover, in 3T3 fibroblast, Krox-20 triggers expression of the myelin genes periaxin and protein zero (P0), a function previously thought to be a specific for Krox-20 in Schwann cells. We show a significant relationship between Krox-20 signaling and the c-Jun NH2-terminal kinase (JNK)c-Jun pathway. We show that this pathway is activated by both NRG-1 and TGFß in Schwann cells and that JNK/c-Jun activity is required for proliferation and death. Importantly, Krox-20 suppresses JNK/c-Jun signaling. This provides Krox-20 with a mechanism for coordinated control of mitogenic and death signaling in developing Schwann cells. Finally, we show that Krox-20 regulates levels of JNK-interacting protein 1 (JIP-1), a scaffold protein that controls the activity of JNK-mediated signaling (Davis, 2000). We propose this as a possible component of the mechanism by which Krox-20 regulates the activity of JNK during Schwann cell development.
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Results |
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In the presence of Krox-20, Schwann cells are resistant to killing by TGFß and growth factor deprivation
TGFß acts as a death signal in immature Schwann cells (Parkinson et al., 2001) and in mice without functional TGFß type II receptors, developmental Schwann cell death is strongly reduced (unpublished data). The escape from death vulnerability and TGFß-induced apoptosis that accompanies myelin sheath formation does not take place in Krox-20-/- mice. To test whether presence of the Krox-20 protein is sufficient to induce death resistance and, in particular, to uncouple TGFß stimulation from induction of apoptosis, we performed survival assays on immunopanned Schwann cells from newborn animals infected with Krox-20/GFP adenovirus or GFP control virus. First, we found that expression of Krox-20 strongly increased survival in response to TGFß plus TNF or TGFß alone (P < 0.002 and P < 0.001, respectively; Fig. 2, A and B). Second, we found that Krox-20expressing cells survived 10% serum withdrawal much better than control cells (Fig. 2 C; Cheng et al., 2001). For both the TGFß and serum withdrawal assays, Schwann cell death was confirmed to be apoptotic by immunolabeling for the activated form of caspase-3 and TUNEL analysis (Parkinson et al., 2001; unpublished data).
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We found that retrovirally sustained Krox-20 expression blocked (P < 0.005) DNA synthesis of Swiss 3T3 cells in response to PDGF-BB and prevented cyclin D1 induction (Fig. 3, A and B). Krox-20 expression also suppressed the proliferation of Swiss 3T3 and NIH 3T3 fibroblasts in donor calf serum (unpublished data). Furthermore, Krox-20 inhibited apoptosis (P < 0.001) of NIH and Swiss 3T3 cells after withdrawal of serum when measured by TUNEL analysis (Fig. 3 C; and not depicted). To our surprise, we found that Krox-20 expression was also sufficient to up-regulate the myelin genes periaxin and P0 in Swiss 3T3 cells. This induction was unambiguous and seen both by immunohistochemistry and Western blotting (Fig. 3, DH).
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Krox-20 causes a selective modulation, rather than general inactivation of NRG-1 or TGFß signaling in Schwann cells
The experiments in Figs. 13 provide a plausible explanation for the increase in cell numbers, proliferation, and death in Krox-20 mutants (Topilko et al., 1994; Zorick et al., 1999) by showing that expression of Krox-20 is sufficient to alter the response of Schwann cells to specific proliferation and death factors present in peripheral nerves. We now set out to examine the underlying molecular mechanisms.
We found that ErbB2 and ErbB3 NRG-1 receptor levels were unchanged in cells infected with Krox-20/GFP adenovirus, and in nerves of Krox-20 null animals (Fig. 4, A and B). Phosphorylation of the ERK1/2 MAPKs, both in unstimulated Schwann cells and in response to NRG-1, was also unchanged by Krox-20 expression, at least in the conditions used in our assay (Fig. 4 A). In addition, basal and NRG-1induced PI3-kinase activity, measured by phosphorylation of Akt, was not significantly affected by Krox-20 expression (Fig. 4 A).
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In conclusion, the power of Krox-20 to inactivate mitogenic and death signaling by NRG-1 and TGFß, respectively, appears to be due to a selective modulation, rather than general inactivation of the signaling pathways activated by these factors. It is also noteworthy that, in Krox-20expressing cells, NRG-1 still activates two of the major kinases involved in NRG-1mediated proliferation, although the cells do not divide. The lack of mitogenic response must therefore derive from interference with other intracellular signaling molecules or pathways.
Krox-20 suppresses basal JNK/c-Jun activity, and inhibits NRG-1 and TGFß-stimulated JNK/c-Jun activation
The JNKc-Jun pathway regulates proliferation and/or death in several cell types (Leppa and Bohmann, 1999; Ham et al., 2000), and in Schwann cells this pathway is required for TGFß-induced apoptosis (Parkinson et al., 2001).
Therefore, we looked for a linkage between Krox-20 and JNK/c-Jun signaling. First, we showed by double label immunohistochemistry that most/all Schwann cell nuclei expressed c-Jun and serine (Ser)63 phosphoc-Jun before myelination (E17), whereas neither antibody bound the Krox-20positive nuclei of early myelinating cells in neonatal nerves, although they continued to label the nonmyelin cells (Fig. 5, NQ; and not depicted). This is consistent with the absence of c-Jun in adult myelinating cells and the observed fall in c-Jun levels during myelination in vivo and myelin induction by cAMP elevation in vitro (Monuki et al., 1989; De Felipe and Hunt, 1994; Stewart, 1995; Shy et al., 1996; Awatramani et al., 2002).
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Third, in experiments involving short exposure to Krox-20 (tests performed 20 h, rather than the usual 48 h, after onset of adenoviral infection), we found that NRG-1 and TGFß-stimulated phosphorylation of c-Jun was suppressed, whereas levels of c-Jun protein still remained unchanged (Fig. 5, B and D). At a later time point (48 h), both phosphoc-Jun and c-Jun levels are reduced by Krox-20 (Fig. 5, C and E). These findings were confirmed by immunolabeling of cells expressing Krox-20 48 h after infection (Fig. 5, FM). These experiments show that Krox-20 expression first blocks growth factor induced c-Jun phosphorylation, whereas prolonged Krox-20 expression also reduces c-Jun protein levels in Schwann cells.
Together, these experiments indicate that the JNKc-Jun pathway is inactivated in individual cells as they start myelination and that this takes place as a consequence of Krox-20 activation. We now asked whether this JNK/c-Jun inactivation was, in turn, sufficient to explain the effects of Krox-20 on NRG-1driven proliferation and TGFß-induced death.
Down-regulation of the JNKc-Jun pathway is sufficient to uncouple NRG-1 receptor activation from mitogenesis
To test whether NRG-1 stimulation of Schwann cell proliferation depended on the JNKc-Jun pathway, we blocked JNK activity in cultured Schwann cells using two methods. First, we used SP600125, a selective inhibitor of JNK1 and JNK2 (Bennett et al., 2001). Having confirmed that 30 µM SP600125 completely prevented JNK1/2 and c-Jun phosphorylation even in the presence of 20 ng/ml NRG-1 (Fig. 6 A), we found that SP600125 blocked Schwann cell DNA synthesis in response to 20 ng/ml NRG-1 in a dose-dependent manner (Fig. 6 B). Second, we performed experiments expressing the JNK binding domain (JBD) of the JNK binding protein JIP-1, which specifically inhibits JNK activity and c-Jun phosphorylation (Harding et al., 2001). In confirmation of this, JBD expression blocked c-Jun phosphorylation, but not ERK1/2 activation, in response to NRG-1 (Fig. 6 C). We found that JBD expression significantly (P < 0.001) inhibited 20 ng/ml NRG-1stimulated BrdU incorporation (Fig. 6 D). Furthermore, JBD expression inhibited Schwann cell death in response to TGFß or serum withdrawal (unpublished data).
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Death prevention by Krox-20 depends on Krox-20mediated inactivation of JNK
Killing of Schwann cells by TGFß depends on the activation of the JNKc-Jun pathway (Parkinson et al., 2001), an event that is inhibited by Krox-20 (Fig. 5). This provides a plausible explanation for the protective effects of Krox-20 against TGFß-induced cell death. It also predicts that enforced activation of this pathway in Krox-20expressing cells should restore their vulnerability to cell death. To reactivate the JNK pathway in Krox-20expressing cells, we used activated forms of either MEKK1 (Olson et al., 1995; Whitfield et al., 2001) or MKK7 (MKK7D; Wang et al., 1998) kinases. Cultured Schwann cells were coinfected with the Krox-20/GFP and either myc-tagged MEKK1- or MKK7D-expressing adenoviruses. Immunolabeling of infected cells showed that >90% of GFP-positive (Krox-20expressing) Schwann cells also expressed MEKK1 (unpublished data). In these double infected cells, MEKK1 and MKK7D reversed the Krox-20mediated inhibition of c-Jun phosphorylation both by Western blot and immunolabeling (Fig. 7 A; and not depicted). To test the effects of MEKK1 or MKK7D on cell death in Krox-20 cells, double infected Krox-20/MEKK1 or Krox-20/MKK7D cells were subjected to the serum withdrawal test (Fig. 2). This showed that most of the cells expressing Krox-20 and a control LacZ adenovirus were protected from death as expected. In contrast, the cells expressing Krox-20/MEKK1 or Krox-20/MKK7D died as expected for uninfected native cells (Fig. 7 B).
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JIP-1, a potential suppressor of JNK activity, is up-regulated by Krox-20
There is evidence that up-regulation of the JNK scaffolding protein JIP-1 (IB1) may in some circumstances inactivate JNK signaling (Bonny et al., 2000; Tawadros et al., 2002). A number of observations were consistent with the idea that JIP-1 is involved in mediating the inhibitory effect of Krox-20 on JNK. First, expression of Krox-20 strongly increased JIP-1 protein levels in Schwann cells in vitro, and we also observed a higher migrating band, which may represent a phosphorylated form of the JIP-1 protein (Fig. 8 A; Meyer et al., 1999). Second, in Krox-20 null mice the levels of JIP-1 protein were substantially reduced, whereas c-Jun levels were elevated (Fig. 8 B). Third, JIP-1 and Krox-20 mRNAs were up-regulated with a similar time course during nerve development, and the JIP-1 mRNA and protein that localized to paranodal regions decreased after nerve cut (Fig. 8, C, F, and G; and not depicted; Nagarajan et al., 2002). A comparable profile of JIP-1 mRNA expression was found using Affymetrix gene arrays (unpublished data). Lastly, expression of JIP-1 selectively reduced (P < 0.0001) the percentage of Ser63 phosphorylated c-Junpositive Schwann cells maintained under basal conditions in vitro, whereas leaving c-Jun protein levels unchanged (Fig. 8, D and E). Together, these experiments suggest that JIP-1 may act as a link in the chain that leads from Krox-20 to inhibition of JNK and reduced phosphoc-Jun.
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Discussion |
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The increased apoptosis in nerves of Krox-20 mutants can also be related to cell-autonomous effects of Krox-20, because we find that Krox-20 expression provides cells with strong protection against apoptotic death. This effect is broad based, in the sense that Krox-20 expression protects Schwann cells from TGFß killing and safeguards both Schwann cells and 3T3 fibroblasts from death by growth factor deprivation. Therefore, it is likely that the death resistance that cells acquire as they start to myelinate (Grinspan et al., 1996; Parkinson et al., 2001) is due to the presence of Krox-20 in these cells. We suggest that in Krox-20-/- nerves, Schwann cells are bereft of this protection and consequently die in large numbers (Zorick et al., 1999).
As to the alternative possibility that increased death in nerves of P12 Krox-20 mutants is due to enhanced competition for axonal survival signals, this is now less attractive, because it is realized that postnatal Schwann cells support their own survival by autocrine signaling, and that death of these cells is not increased even when axons are cut (Grinspan et al., 1996; Cheng et al., 1998; Dowsing et al., 1999; Meier et al., 1999).
Krox-20 shares similarities with master regulatory genes
Unexpectedly, constitutive expression of Krox-20 caused the myelin proteins periaxin and P0 to be expressed in 3T3 fibroblasts, cells that do not normally express these proteins, and are unrelated to Schwann cells. Periaxin and P0 show extremely restricted distribution in vivo, being largely confined to myelinating Schwann cells in the adult peripheral nervous system (Scherer et al., 1995; Lee et al., 1997).
We also observed that Krox-20 induces growth arrest and a decrease in cyclin D1 levels not only in Schwann cells but also in fibroblastic Swiss 3T3 cells treated with PDGF, a classical mitogen for these cells (Withers et al., 1995). This agrees with previous data showing c-Jun transactivates the cyclin D1 promoter (Sabbah et al., 1999; Wisdom et al., 1999). Furthermore, Krox-20 protects 3T3 cells, like Schwann cells, from death induced by growth factor deprivation.
The present experiments and previous work (Nagarajan et al., 2001; Parkinson et al., 2003) show that Krox-20 activates a large range of myelin differentiation genes and proteins, organizes cell cycle exit and protects from death. It can also execute some of its key actions in a heterologous cell. Therefore, Krox-20 acts in many ways in a manner expected of a master regulator for myelin Schwann cells, and has several of the properties of master regulatory genes such as MyoD, the neural basic helix-loop-helix genes, or PPAR (Davis et al., 1987; Tontonoz et al., 1994; Lee et al., 1995; Lo et al., 1998; Zorick et al., 1999; Sabourin and Rudnicki, 2000).
Krox-20 suppresses the JNKc-Jun pathway
We find that constitutive expression of Krox-20 in cultured Schwann cells results in down-regulation of the JNKc-Jun pathway under basal conditions, and suppression of JNK/c-Jun activation by NRG-1 and TGFß. This inhibitory interaction between Krox-20 and c-Jun is likely to reflect the action of Krox-20 in vivo. The principal evidence is the excellent negative correlation we see between expression of Krox-20 on the one hand and c-Jun and phosphorylated c-Jun on the other in the nuclei of individual Schwann cells in neonatal nerves. Earlier work also supports this conclusion. Adult myelinating cells express Krox-20 but not c-Jun, although it is detectable in nonmyelinating cells (Shy et al., 1996). After nerve transection, c-Jun levels rise as Schwann cells dedifferentiate and Krox-20 levels fall, whereas during regeneration c-Jun falls and Krox-20 expression increases as cells establish new myelin sheaths (De Felipe and Hunt, 1994; Stewart, 1995; Shy et al., 1996; Topilko and Meijer, 2001).
Suppression of the JNKc-Jun pathway reduces proliferation and death in Schwann cells
We find that inhibition of JNK in two unrelated ways, i.e., by the small molecular blocker SP600125 or by the JBD of JIP-1, uncouples NRG-1 stimulation from DNA synthesis. The JNKc-Jun pathway has not previously been implicated in Schwann cell proliferation. The expression pattern of c-Jun is, however, consistent with such a role. c-Jun is high in embryonic nerves before myelination when proliferation is ongoing, low in mature nerves where proliferation is absent and high in transected nerves (previous paragraph). Although our experiments indicate that JNK/c-Jun activity is necessary for Schwann cell proliferation, at least in response to NRG-1, it is not sufficient. We see this in Schwann cells maintained in medium without mitogens in vitro and in the distal stump of nerves several weeks after transection. In both cases, proliferation is low or absent but c-Jun expression is relatively high.
The observation that the JNKc-Jun pathway is required for NRG-1driven Schwann cell proliferation indicates that the antiproliferative effect of Krox-20 can be attributed to the ability of this transcription factor to inactivate this pathway, as evidenced by down-regulation of c-Jun protein, phosphorylated c-Jun, and phosphorylated JNK. Although the data indicate that suppression of the JNKc-Jun pathway is sufficient to account for the effects of Krox-20 on proliferation, it is possible that Krox-20 has additional actions in Schwann cells that also contribute to cell cycle exit. This is suggested by the Krox-20mediated induction of the cell cycle inhibitor p27.
We have shown previously that TGFß-induced death involves phosphorylation of c-Jun, that an active form of c-Jun kills Schwann cells and that a dominant negative form of c-Jun inhibits TGFß-induced death (Parkinson et al., 2001). Therefore, as in some other cell types (Kyriakis and Avruch, 2001; Shaulian and Karin, 2002), the JNKc-Jun pathway is a component of at least two signaling cascades in Schwann cells, those that promote death and those that promote proliferation. This dual function allows Krox-20 to suppress both cell division and death through a single action, namely inactivation of the JNKc-Jun pathway.
Regulation of JIP-1 by Krox-20 during Schwann cell development
JNK signaling is regulated by the balance between upstream activating kinases such as MKK4/7 and protein phosphatases, which act to turn off JNK activity (Camps et al., 2000; Weston and Davis, 2002). Another layer of JNK regulation has been revealed with the identification of JIP-1, which acts as a scaffold protein and binds the mixed lineage kinase MLK3, the MAPK MKK7, and JNK1/2 (Dickens et al., 1997; Whitmarsh et al., 1998).
JIP-1 potentiates the activation of JNK by MKK7. However, JIP-1 can also inhibit JNKc-Jun interactions (Dickens et al., 1997), which would lead to reduced c-Jun phosphorylation, and there are a number of reports that increased levels of JIP-1 suppress JNK signaling (Dickens et al., 1997; Bonny et al., 2000; Tawadros et al., 2002).
Our finding that JIP-1 is present and developmentally regulated in peripheral nerve and a target of Krox-20 is of great interest, because this may be part of the mechanism by which Krox-20 suppresses JNK/c-Jun activation. Notably, neither expression of the JBD region or full-length JIP-1, both of which reduce c-Jun phosphorylation, affected c-Jun protein levels in Schwann cells. The mechanism by which Krox-20 suppresses c-Jun protein therefore remains to be discovered. The localization of JIP-1 in the paranodal region of the myelinating Schwann cells is also of interest. JIP-1 binds the RhoA exchange factor, RhoGEF190 (Meyer et al., 1999), and a similar localization was observed for RhoA in Schwann cells (Scherer and Gutmann, 1996), raising the possibility that JIP-1 may function in regulation of Rho activity in Schwann cells.
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Materials and methods |
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Cell culture
Schwann cells were prepared from the sciatic nerve and brachial plexus from newborn or 3-d-old rats (Brockes et al., 1979; Morgan et al., 1991). Schwann cells for TGFß-induced apoptosis studies were prepared by immunopanning and used directly (Dong et al., 1999). Schwann cells were cultured unless otherwise stated in serum-free supplemented medium (Jessen et al., 1994) containing 10-6 M insulin, referred to as defined medium (DM). For adenoviral infection experiments, serum-purified Schwann cells were plated at a density of 5,000 cells in a 15-µl drop on poly-D-lysine/laminincoated coverslips. For preparation of adenovirally infected cells for Western blot, serum-purified, or immunopanned Schwann cells were infected as described previously (Parkinson et al., 2001). For retroviral infection of Schwann cells, cells were cultured in DME supplemented with 3% FCS/2 µM forskolin/20 ng/ml NRG-1, and infected using retroviral supernatant from GP+E packaging cells as described previously (Parkinson et al., 2001, 2003). NIH 3T3 and Swiss 3T3 fibroblasts were cultured in DME/10% donor calf serum for infection using retroviral supernatants. Transient transfections and Krox-20 genotyping were prepared as described previously (Schneider-Maunoury et al., 1993; Parkinson et al., 2001, 2003).
Cell survival assays
Schwann cells were infected with GFP control, Krox-20/GFP, or JBD-expressing adenovirus. 24 h later, cells were changed into DM. 6 h later, time 0 controls were fixed, whereas remaining cells were changed into DM or medium containing a combination of 20 ng/ml TGFß1 plus 40 ng/ml TNF or 20 ng/ml TGFß1 alone in DM. The relationship between cell survival and death in this assay has been extensively characterized and is reciprocal and cell survival was assessed as described previously (Meier et al., 1999; Parkinson et al., 2001). For 24-h survival assays with TGFß1 plus TNF
, percentage of survival is shown relative to time 0 controls. For TGFß1 assays, percentage of survival is relative to 48 h untreated controls. For survival assays after serum withdrawal, Schwann cells were adenovirally infected in DME containing 10% FCS. 24 h after infection, time 0 controls were fixed, whereas remaining cells were changed into DME alone; cells were fixed after 72 h. Results shown are pooled from three independent experiments. TUNEL analysis and immunolabeling for activated caspase-3 were performed as described previously (Gavrieli et al., 1992; Parkinson et al., 2001).
Immunocytochemistry
Immunolabeling with BrdU, P0, and JIP-1 antibodies was performed as described previously (Morgan et al., 1991; Stewart et al., 1993; Willoughby et al., 2003). For all other antibodies, cells were fixed in 4% PFA in PBS, pH 7.5, for 10 min. For immunolabeling with Ser63 phospho-Jun antibodies, cells were permeabilized in 0.5% Triton X-100/PBS for 5 min followed by a block of 50% goat serum/1% BSA/PBS for 30 min; the antibody was diluted in 1% BSA and left overnight at 4°C. For other antibodies, after fixing cells were permeabilized and blocked in antibody diluting solution (PBS containing 10% calf serum, 0.1 M lysine, 0.2% sodium azide) supplemented with 0.2% Triton X-100 for 30 min. Primary and secondary antibodies were diluted and applied in antibody diluting solution, with the secondary antibody conjugated to either FITC or Cy3. Coverslips were mounted in Citifluor (Citifluor Ltd.), and examined at RT with a fluorescence microscope (model Eclipse E800; Nikon). Images were captured at 40x magnification, using a digital camera (model DXM1200; Nikon), and ACT-1 acquisition software (Nikon). Pictures were digitalized using UMAX PowerLookIII scanner and figures were prepared using Adobe Photoshop version 5.0.
Western blot analysis
40 µg of protein extracts were electrophoresed on 10% SDS-polyacrylamide gels, transferred onto nitrocellulose membranes (Hybond ECL; Amersham Biosciences), blocked with 5% fat-free milk in TBS/0.1% Tween 20, and incubated with primary antibodies in this block solution. Membranes were washed in TBS/Tween 20 and secondary antibody added in this solution. Specific protein complexes were revealed using ECL Plus chemiluminescent reagent (Amersham Biosciences).
PCR
For PCR analysis, RNA was isolated from sciatic nerves of rats at E14, E18, P7, P12, and P12 cut at P7, using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. First strand cDNA was synthesized from total RNA using oligo dT primers (Promega) and Avian Myeloblastosis Virus reverse transcriptase (Promega). Oligonucleotide primers (Invitrogen) were used to amplify sequences containing the JIP-1 (5'-CGACTGTCTGTCATCCCCAG-3' and 5'-CATAGACAGTGGCAGAGTCG-3') and GAPDH (5'-ACCACAGTCCATGCCATCAC-3' and 5'-TCCACCACCCTGTTGCTGTA-3'). The PCR amplification program consisted of denaturation at 95°C for 30 s, annealing at 55°C for 1 min, and extension at 72°C for 1 min for 35 cycles.
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Acknowledgments |
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This work was supported by a Wellcome Trust Program grant to K.R. Jessen and R. Mirsky.
Submitted: 21 July 2003
Accepted: 16 December 2003
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References |
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