The Burnham Institute (formerly the La Jolla Cancer Research Foundation), La Jolla, California 92037
Keratins 8 (K8) and 18 (K18) are major components of intermediate filaments (IFs) of simple epithelial cells and tumors derived from such cells. Structural cell changes during apoptosis are mediated by proteases of the caspase family. During apoptosis, K18 IFs reorganize into granular structures enriched for K18 phosphorylated on serine 53. K18, but not K8, generates a proteolytic fragment during drug- and UV light-induced apoptosis; this fragment comigrates with K18 cleaved in vitro by caspase-6, -3, and -7. K18 is cleaved by caspase-6 into NH2-terminal, 26-kD and COOH-terminal, 22-kD fragments; caspase-3 and -7 additionally cleave the 22-kD fragment into a 19-kD fragment. The cleavage site common for the three caspases was the sequence VEVD/A, located in the conserved L1-2 linker region of K18. The additional site for caspases-3 and -7 that is not cleaved efficiently by caspase-6 is located in the COOH-terminal tail domain of K18. Expression of K18 with alanine instead of serine at position 53 demonstrated that cleavage during apoptosis does not require phosphorylation of serine 53. However, K18 with a glutamate instead of aspartate at position 238 was resistant to proteolysis during apoptosis. Furthermore, this cleavage site mutant appears to cause keratin filament reorganization in stably transfected clones. The identification of the L1-2 caspase cleavage site, and the conservation of the same or very similar sites in multiple other intermediate filament proteins, suggests that the processing of IFs during apoptosis may be initiated by a similar caspase cleavage.
APOPTOSIS, or programmed cell death, is a process that
enables multicellular organisms to eliminate cells
that are damaged or mislocated or have become
superfluous (Ellis et al., 1991 Apoptosis is characterized by ultrastructural changes
that include actin cytoskeletal disruption, membrane
blebbing, decreases in adhesion and intercellular contacts,
chromatin condensation, nuclear fragmentation, and packaging of the nuclear fragments into membrane-enclosed
apoptotic bodies (Wyllie et al., 1980 Intermediate filaments (IFs)1 are composed of members
of a family of proteins that organize to form 10-nm filaments and share sequence homology and structural features (Fuchs and Weber, 1994 In this study, we show that K8/K18 filaments are dramatically reorganized during apoptosis, and that this reorganization is associated with the phosphorylation of K18
on serine 53. Furthermore, K18, but not K8, is cleaved by
caspases at the sequence VEVD/A within the region that
links two major Cell Lines, Plasmids, and Antibodies
SNG-M cells are from a human endometrial adenocarcinoma (Ishiwata et
al., 1977 Plasmid pK187 consists of the full-length K18 cDNA cloned into the
EcoRI site of pGEM1 with the 5 We used the following antibodies: rabbit polyclonal antibody 1589 against EndoB, which cross-reacts with K18 and was prepared, characterized, and reacts like the originally described antiserum (Oshima, 1981 Full-length cDNAs encoding caspases-3, -6, and -7 were cloned into
pET vectors (Invitrogen, San Diego, CA) and expressed in Escherichia
coli strain BL21 (DE3)pLysS (Orth et al., 1996 Immunofluorescence
SNG-M cells grown on coverslips were treated with etoposide (250 µg/ml)
or DMSO solvent alone for 24 h, washed three times in PBS, and fixed in
methanol for 5 min ( In Vitro Transcription and Translation of K8, K18,
and EndoB
K8 and K18 proteins were synthesized using separated or coupled transcription and translation reactions as indicated. Transcription was performed using 2 µg of linearized plasmid DNA (XbaI for pK811, HindIII
for pK187, and BamHI for SP64B7) in a 50-µl volume as previously described (Oshima et al., 1986 K18 and EndoB In Vitro Cleavage Assay
1 µl of the radioactive translation product was incubated with 1 µl of purified recombinant caspase-6 (0.2 mg/ml), caspase-3 (0.15 mg/ml), or
caspase-7 (0.13 mg/ml) at the indicated dilutions in a 40-µl volume containing 50 mM Hepes, 0.1 M NaCl, 10% sucrose, 0.1% CHAPS, and 10 mM DTT at 37°C for the indicated times. Reaction was stopped by adding
SDS-PAGE sample buffer (Laemmli, 1970 Site-directed Mutagenesis
Site-directed mutagenesis of the aspartate to glutamate at position 238 of
K18 was achieved using the QuickChangeTM Site-Directed Mutagenesis
Kit (Stratagene, La Jolla, CA) following the instructions of the supplier.
The two complementary oligonucleotides, GTTGACCGTGGAGGTAGAGGCCCCCAAATCTCAGG and CCTGAGATTTGGGGGCCTCTACCTCCACGGTCAAC, containing the desired mutation were used.
A 50-µl reaction was subjected to 12 cycles of 95°C 30 s, 55°C 1 min, and
68°C 10 min. 10 U of DpnI restriction enzyme was added to each amplification reaction and were incubated for 1 h at 37°C. The reaction was used
to transform Epicurean Coli XL1-blue competent cells. Plasmid DNA was
purified from seven colonies and sequenced. All seven plasmids contained
the desired mutation without other changes.
Gel Electrophoresis and Immunoblotting
Cells were lysed in SDS-PAGE sample buffer, and equal amounts of protein were separated on 15% polyacrylamide gels. Proteins were transferred electrophoretically to nitrocellulose (Towbin et al., 1979 Apoptosis Assays
Apoptosis was induced by UV irradiation from a Stratalinker (Stratagene) UV source by exposing cells in complete medium to 120 millijoules
of UV light. Apoptosis was also induced by treatment of semiconfluent
cultures with the drugs etoposide (Sigma Chemical Co.) at 250 µg/ml for
SNG-M and 125 µg/ml for HR-9 and daunomycin (Sigma Chemical Co.)
at 6 µg/ml for SNG-M and 2 µg/ml for HR-9.
Apoptosis was confirmed using two approaches. First, DNA degradation was observed by agarose gel electrophoresis. Low molecular weight
genomic DNA was extracted from adherent and floating cells induced to
undergo apoptosis (Frisch and Francis, 1994 Cell Transfections
HR-9 cells were transfected by the calcium phosphate precipitate method
as described previously (Oshima et al., 1990 Reorganization of K8/K18 Filaments During Apoptosis
K8 and K18 are the characteristic IF proteins expressed in
simple epithelia. The two proteins form heterodimers that
polymerize to generate IFs and bundles of IFs that normally extend from the nucleus to the plasma membrane.
When the epithelial cell line SNG-M is treated with etoposide for 24 h, apoptotic cells begin to detach from the dish.
Most of the cells that remained attached after this length
of drug treatment contained a normal cytoskeletal network pattern (Fig. 1, A, C, and E). The nuclear morphology of these cells was normal (Fig. 1, B, D, and F). However, a small percentage of the cells had alterations in the
organization of the K18 filament network. Some cells
showed a K18 cytoskeleton in which the filament network
appeared aggregated or collapsed, and others contained
K18 in aggregates or spots spread throughout the cytoplasm (Fig. 1, A, C, and E). A gradation in the size of the
granules was observed between different cells. Cells could be detected with K18 organized in both small spots still associated with the filament network (Fig. 1 A) and as large
granules (Fig. 1 E). Most of the cells with disorganized
keratin patterns contained fragmented nuclei, a characteristic of advanced apoptosis (Fig. 1 E, lower right corner).
All of the cells with fragmented nuclei contained granular
K18 organization. However, not every cell with disorganized filaments had fragmented nuclei, which suggests that the reorganization of K18 filaments is an event that occurs
before obvious nuclear fragmentation. Furthermore, some
cells had both a few granules and extended filament bundles, suggesting that keratin granule formation may start
locally within a portion of the cell.
Cells were also stained with fluorescent annexin V, a
marker that reflects the membrane changes that occur
early during apoptosis (Thiagarajan and Tait, 1990
Specific Cleavage of K18 During Apoptosis
To determine the integrity and phosphorylation state of
K18 proteins during apoptosis, we analyzed the K18 protein by Western blotting of SNG-M cells induced to undergo apoptosis by treatment with etoposide. For this purpose, subconfluent cultures of SNG-M cells were incubated
with etoposide (250 µg/ml), and adherent and floating
cells were collected and combined at different times of
etoposide incubation. Whole cell lysates were analyzed by
western blotting (Fig. 3). In parallel experiments, typical
apoptotic nuclear fragmentation was analyzed by DNA
staining with DAPI. Treatment of SNG-M cells with etoposide resulted in progressive induction of apoptosis. After 36 h of treatment, >50% of the cells exhibited apoptotic nuclei. After 60 h, 90% of the cells were apoptotic
(Fig. 4 A). Western blot analysis showed that etoposide treatment also resulted in a progressive degradation of
K18 and the appearance of a proteolytic product of ~23
kD (Fig. 3 A, K18 B).
Analysis of K8 expression during etoposide treatment of
SNG-M using the same membrane probed with monoclonal antibody M20 showed little change in the amount of
K8 during apoptosis (Fig. 3 C). By contrast, total K18 degradation was detected with the K18 monoclonal antibody
CK5 (Fig. 3 D). However, the K18 B fragment was not detected with the CK5 antibody.
To follow the phosphorylation state of K18 during apoptosis, the membrane shown in Fig. 3 A was reprobed with
antibody 3055, which preferentially recognizes K18 phosphorylated on serine 53 (P-K18) (Fig. 3 B). A residual signal for K8 remained on this filter. A careful comparison of
the proteins recognized by polyclonal K18 antibody (Fig. 3
A) and monoclonal K18 (Fig. 3 D) confirmed that P-K18
comigrated with K18 (Fig. 3 B, lane 1). Importantly, the
K18 B fragment found in etoposide-treated SNG-M cells
was preferentially recognized by antibody 3055 (Fig. 3 B,
lanes 2-8). Treatment with etoposide resulted in the progressive disappearance of the full-size P-K18 and the appearance of P-K18 fragment B. This result is interesting in
light of the preferential staining of the K18 granules by the
P-K18 antibody, 3055, observed in stained cells (Fig. 2).
Densitometric analysis of the bands corresponding to K18
in Fig. 3 A and P-K18 in Fig. 3 B suggests that P-K18 disappeared faster than K18 (Fig. 4 C), while the P-K18 B
fragment appeared sooner than the K18 B fragment (Fig. 4
B). However, the 3055 antibody is more sensitive for the
detection of the K18 B fragment than the 1589 polyclonal
antibody, which was prepared against the whole mouse
K18 protein. The earlier detection of the P-K18 B fragment may be attributed, at least in part, to the greater sensitivity of the 3055 antibody. However, the P-K18 B fragment reached a peak of accumulation earlier than the total
K18 B fragment and then declined (Fig. 4 B). The decline
in the P-K18 B fragment, even while the K18 B fragment
continues to increase, suggests that nonphosphorylated
K18 can be cleaved. Furthermore, expression of K18 with
an alanine substitution for serine 53 in mouse epithelial cells confirmed that phosphorylation of this residue is not
required for cleavage during apoptosis (see below). Nevertheless, P-K18 may still be a preferential substrate for
cleavage.
Apoptosis was also induced in mouse HR-9 parietal endodermal cells treated with daunomycin. HR-9 treatment
with daunomycin resulted in EndoB (mouse K18) cleavage and the appearance of a 24-kD proteolytic fragment
(EndoB b) (Fig. 3 E) after 24 h of treatment. This fragment accumulated in parallel to EndoB disappearance. After 48 h, almost all the EndoB protein was cleaved to
the EndoB b fragment.
To study whether K18 cleavage is a generalized event
during apoptosis and is preferentially associated with apoptotic cells, we analyzed K18 integrity after the induction of
apoptosis using different stimuli in human (SNG-M) and
mouse (HR-9) cell lines. Apoptosis was induced by treatment of the cells with etoposide, daunomycin, and UV
light. After 24 h of etoposide or daunomycin treatment, or
24 h after brief exposure to UV light, whole cell lysates
were prepared from adherent and floating cells and were analyzed by immunoblot analysis using K18 polyclonal antibody (Fig. 5 A). Most K18 was present as an intact full-size form in the adherent cells in both HR-9 and SNG-M
cells subjected to the different stimuli, whereas floating
cells exhibited mainly the K18 proteolytic fragment (Fig. 5
A). However, a small amount of cleaved K18 among the
total adherent cell population could easily escape detection. Thus, this experiment does not rule out that the granular aggregates detected in a small fraction of adherent SNG-M cells at early times of etoposide treatment by immunofluorescent staining (Fig. 1) contain some cleaved
K18. This experiment confirms that different apoptotic
stimuli result in similar K18 cleavage in the nonadherent,
apoptotic cells.
K18 cleavage was also detected in apoptotic cells of human breast carcinoma MB-231, monkey COS cells, and
mouse MT mammary tumor cells (not shown). These results indicate that K18 cleavage is a general characteristic
of apoptotic epithelial cells. The K18 cleavage observed in
the floating cells is specific for apoptosis and not due to
simple detachment of the cells from the plate because no
K18 cleavage was detected in log phase cells cultured in
suspension for up to 48 h (data not shown).
Apoptosis in the adherent and floating cell populations
was confirmed by the analysis of low molecular weight genomic DNA. For every stimulus used for apoptosis, the
DNA extracted from the adherent fraction was largely intact (Fig. 5 B, lanes 2, 4, 6, 9, 11, and 13). However, floating cells yielded degraded DNA (Fig. 5 B, lanes 3, 5, 7, 10,
12, and 14) and, in some cases, exhibited the typical DNA
ladder. This indicates that the different stimuli resulted in
the production of apoptotic cells in the floating fraction
and live cells in the adherent fraction. The nonextractable, high molecular weight DNA of apoptotic cells and of adherent cells migrates very slowly at the top of similar gels
(data not shown). These data are consistent with other indicators of apoptosis, such as nuclear morphology and annexin V staining.
Caspases Specifically Cleave K18 In Vitro
Because caspase activation is associated with apoptosis,
the K18 cleavage product found in apoptotic cells was
compared to isolated K8/K18 incubated in vitro with purified recombinant caspase-6. Human K18 was detected
with the 3055 antibody, which reacted very weakly with
mouse K18. Purified K18 migrated slightly slower than K18 in whole-cell lysates because of the large difference in
total protein load (Fig. 5 C, lanes 1 and 4). When mixed
with a whole-cell lysate, isolated K18 comigrated with the
cellular K18 (Fig. 5 C, lanes 1 and 3). Purified K18 incubated with caspase-6 resulted in a proteolytic fragment
that comigrated with the 23-kD fragment in apoptotic cells
(Fig. 5 C, lanes 5 and 7). This result indicated that the K18
B fragment generated in vivo during apoptosis may be the
product of caspase cleavage.
To characterize the cleavage of K18 by caspases in more
detail, an in vitro cleavage assay was performed with
[35S]methionine-labeled K18 and K8 and with purified recombinant caspase-6, -3, and -7. Reticulocyte lysate-translated K8 and K18 were incubated separately with varying
amounts of each caspase; the resulting products were analyzed by SDS-PAGE and fluorography. All three caspases
specifically cleaved K18 (Fig. 6 A, lanes 7-10, 15-18, and
23-26) but not K8 (Fig. 6 A, lanes 2-5, 11-14, and 19-22)
to generate a 23-kD K18 B fragment. Caspase-6 was the
most effective because it cleaved at the highest dilution. It
also generated fragment C as a major product and, at high
caspase concentrations, a trace amount of fragment D
(Fig. 6 A, lane 7). Caspase-3 generated primarily fragments B and D and trace amounts of fragment C. The fragment D product is ~19 kD in size (Fig. 6 A, lanes 15-
17, fragment D). In addition, caspase-3 but not caspase-6
generated a large fragment that migrated only slightly
faster than full-length K18 (Fig. 6 A, lanes 15-18, fragment
A). At low concentrations of caspase-3, the A fragment
was produced preferentially (Fig. 6 A, lane 18). This pattern of appearance suggests that fragment D may be the
product of further digestion of fragment A. Caspase-7 generated a pattern very similar to caspase-3 (Fig. 6 A, lanes 23-26). However, ~100-fold more caspase-7 was necessary to generate an equivalent digestion to caspase-3.
EndoB was also cleaved by the three proteases, resulting in a similar proteolytic pattern as K18, with some difference in the mobility of the fragments (Fig. 6 B, a, b, c,
and d). Fragment b had slightly slower electrophoretic mobility than K18 B, whereas c and d displayed faster mobility than C and D, respectively. The differences in mobility
from K18 fragments likely reflect small differences in
amino acid composition between K18 and EndoB, which is
also manifested by the faster mobility for K18, even though it is seven residues larger than EndoB (Fig. 6 B,
lanes 1 and 5) (Oshima et al., 1986 Mapping of the Cleavage Sites
K18 cleavage by caspases-3, -6, and -7 all generated the B
proteolytic fragment found in apoptotic epithelial cells. In
addition, caspases-3 and -7 also preferentially cleaved K18
to generate the A fragment with a slightly faster mobility
than K18. To map the location of both cleavage sites,
COOH-terminal deletions of the K18 and EndoB proteins
were generated by transcribing and translating the cDNAs
truncated within the coding regions at several different restriction enzyme sites (Fig. 7, A and C). Translation of
these truncated mRNAs resulted in K18 proteins of 339 residues (K18-339) and 269 residues (K18-269), instead of
the full-length 430 amino acids. These truncated forms of
K18 were cleaved with caspases-6, -3, and -7 to analyze the
proteolytic profile generated. Cleavage of K18-339 with
caspases-3 and -7 (Fig. 7 B, lanes 7 and 8) did not produce
any slightly smaller fragment that might correspond to
fragment A of the full-length protein. Thus, the cleavage
site of caspases-3 and -7 that generates the A fragment likely resides between residue 339 and the COOH-terminal end. All three caspases generated the B fragment from
both K18-339 and K18-269. Thus, the B fragment originates from the NH2-terminal half of K18, from the cleavage just upstream of residue 269 (Fig. 7 B, lanes 10 and
11). If one caspase-6 site is responsible for the generation
of the B and C fragments from wild-type K18, a severely
truncated C fragment might be expected by digestion of K18-339. However, this fragment was not detected, perhaps because it may migrate near the buffer front with the
excess free, radioactive methionine.
The same approach was used to map the cleavage sites
for caspases in EndoB. Three deletion mutants of EndoB
in the COOH-terminal were generated and cleaved with
the different caspases. The full-length EndoB is 423 residues. Truncation of the EndoB cDNA with AvaI, ScaI,
and PvuII generated proteins of 316, 262, and 191 residues, respectively (Fig. 7 C). Cleavage of EndoB-316 with
caspases-6, -3, and -7 (Fig. 7 D, lanes 6-8) produced the b
fragment without any indication of an a fragment derivative. Thus, as for K18, the site that generates the a fragment must have been deleted in EndoB-316. EndoB-262 is
slightly larger than the b fragment, and the cleavage with
all three caspases still generated the b fragment (Fig. 7 D,
lanes 10-12), indicating that the cleavage site that generates the b fragment is upstream but close to amino acid
262. This places the site close to the conserved VEVD/A
sequence also found in the K18 protein (Fig. 7, A and C).
EndoB-191 is smaller than the b fragment, and no site of
cleavage for any of the caspases was found in the first 191 residues of the protein.
Mutagenesis of the K18 Caspase Cleavage Site
Caspases are considered to require an aspartic acid residue at the P1 position immediately upstream of the cleavage site for efficient cleavage. To assess whether the
VEVD238 sequence in the linker region of K18 is the target for caspases, we mutated the aspartate to glutamate. A
[35S]methionine-labeled K18 mutant protein (K18-D238E)
was synthesized by coupled transcription and translation.
The sensitivity of this protein to digestion by caspases-3
and -6 was compared to wild-type K18. Caspase-3 cleaved
K18 in a time-dependent manner generating the A, B, and
D fragments as expected (Fig. 8 A, lanes 1-7). By contrast,
K18-D238E produced only the A fragment, while the B and D fragments were absent (Fig. 8 A, lanes 8-14). Thus,
changing the aspartate to a glutamate in the sequence
VEVD appears to abolish the internal cleavage but not
the COOH-terminal domain digestion. The mutant K18-D238E protein was also resistant to caspase-6 digestion (Fig. 8 B). In this case, caspase-6 does not cleave the
COOH-terminal domain site recognized by caspase-3 and -7.
The identification of the VEVD/A sequence in K18 as
the target of caspase digestion provides the information
necessary to interpret the origin of all four fragments generated by the different caspases (Fig. 8 C). Digestion of
K18 with caspase-6 generated the B, NH2-terminal fragment of 238 residues with a molecular mass of 26.3 kD.
The estimated size of the B fragment was 23 kD from gel
mobility. These values appear within reasonable agreement, considering the slightly anomalous migration behavior of K18. The C fragment is predicted to be 192 residues
with a molecular mass of 21.8 kD, which is very close to
the value of 22 kD estimated from the gels. The exact location of the COOH-terminal cleavage site, which generates
fragments A and D by caspase-3 and -7, is not known.
However, the difference between fragments C and D is ~3
kD, which would place the site at the COOH-terminal,
non- Expression of K18-D238E and K18-S53A in
Epithelial Cells
To determine the importance of the primary K18 caspase
cleavage site and the phosphorylation of ser53 for K18
cleavage in cells during apoptosis, HR-9 mouse parietal
endodermal cells were transfected with expression constructions for the wild-type K18, the ser53 Apoptosis was induced by treatment with daunomycin,
and after 24 h, cell lysates were prepared from adherent
and floating cells and were analyzed by Western blot with
polyclonal antibody 1589, which recognizes both K18 and
EndoB. As expected, most of the EndoB present in the
adherent population is the full-length EndoB (Fig. 9 A,
lane 2), whereas the floating fraction contains mainly the
proteolytic fragment, EndoB b (Fig. 9 A, lane 3). K18-transfected HR-9 cells express both K18 and EndoB (Fig.
9 A, lane 4), and both remain in the adherent cells after
treatment with daunomycin (Fig. 9 A, lane 5). However,
EndoB and K18 disappeared in the floating cells to generate the proteolytic fragments EndoB b and K18 B (Fig. 9
A, lane 6), indicating that both endogenous EndoB and
exogenous K18 are cleaved in apoptotic HR-9 cells. K18-D238E is also present in the adherent population of transfected HR-9 cells (Fig. 9 A, lane 8) along with EndoB.
However, whereas EndoB disappears in the floating fraction, K18-D238E still remains (Fig. 9 A, lane 9), showing
resistance to cleavage. This result is consistent with use of
the caspase cleavage site VEVD/A in vivo. A small amount of K18-D238E cleavage product is detected when
the 3055 antibody is used (Fig. 9 B, lane 9), indicating that
this conservative mutation dramatically decreases the sensitivity of K18 to cleavage but does not completely abolish
it in cells. Nevertheless, the high level of full-length K18-D238E in nonadherent, apoptotic HR-9 differs dramatically from the behavior of wild-type K18, EndoB, or K18-S53A (see below) and confirms that the primary caspase cleavage site identified in vitro is also used in cells.
Induction of apoptosis in HR-9 cells transfected with
K18-S53A resulted in the cleavage of both EndoB and
K18-S53A in the floating cells (Fig. 9 A, lane 12), indicating that phosphorylation of ser53 is not required for the
caspase cleavage of K18, at least within the context of endogenous wild-type EndoB. Absence of phosphorylation in K18-S53A was confirmed by Western blot with antibody 3055 (Fig. 9 B, lanes 10-12).
Keratin Filament Organization of K18-S53A
and K18-D238E
Transient expression of wild-type K18, K18-S53A, and
K18-D238E all resulted in cells that appeared to incorporate the human K18 proteins into the endogenous keratin
network of HR-9 cells without significant perturbations
(data not shown). HR-9 cells that continued to express either wild-type K18 or K18-S53A after drug selection were
isolated and stained with the CK5 monoclonal antibody or
the 3055 antibody to visualize K18 and P-K18. Both K18
and K18-S53A were found in the endogenous mouse keratin filament system (Fig. 10, A and B, antibody 1589 staining not shown). Some of the cells expressing wild-type K18
contained detectable P-K18 in a filamentous organization
(Fig. 10 D), although in most cells the staining was quite
weak. As expected, no specific staining of K18-S53A was
detected by the 3055 antibody (Fig. 10 E). Only background staining, equivalent to that produced by the secondary antibody or by cells that ceased K18 expression,
was detected (Fig. 10 F, bottom left).
Cells stably transfected with K18-D238E grew slowly,
generated aberrant large cells, and appeared to be selected
for the loss of expression because the proportion of cells
that stained with CK5 antibody decreased as the culture
expanded. The resulting population contained only ~30%
K18-positive cells in the best clone. Multiple additional
clones failed to sustain detectable K18-D238E expression.
Immunofluorescent staining revealed positive cells with
aggregates of keratin filaments in an altered organization (Fig. 10 C) as well as protein distributed in a filamentous
organization. The nuclei of these cells did not have alterations associated with apoptosis, and Western blotting
confirmed the absence of detectable cleavage of EndoB in
growing cultures (Fig. 9 A, lane 7). Thus, this aggregation
was not due to induction of apoptosis. These aggregates
were preferentially reactive with the 3055 antibody specific for P-K18 (Fig. 10, F-I). The remaining filamentous
K18-D238E (Fig. 10 C) reacted very weakly with the 3055 antibody. In addition, the 3055 antibody permitted the
double staining for the mouse complementary type II keratin, EndoA, with a monoclonal antibody. EndoA was
colocalized in the aggregates (Fig. 10, J-L) as well as in a
filamentous organization in the same cell. The aggregates of P-K18-D238E and EndoA were commonly localized at
the edge of the nucleus.
Comparison of the amounts of K18-D238E and K18-S53A by immunoblotting suggests that K18-D238E is not
expressed at an excessively high level per cell, even after
normalizing for the percentage of K18-positive cells
(100% for HR-9 K18S53A and ~30% for HR-9 K18-D238E). These results suggest that the K18-D238E protein may act as a subtle dominant negative mutant. The
presence of normal-appearing keratin organization in
some of both the stably transfected cells and all the transiently transfected cells suggests that this effect may be
more subtle than many dominant negative acting mutants of keratins (Kulesh et al., 1989 We have explored the fate of keratin intermediate filament proteins in apoptotic epithelial cells and have discovered that a dramatic reorganization of the keratin cytoskeleton precedes morphological nuclear disruption during
apoptosis and is associated with the phosphorylation of
K18 on serine 53. Degradation of simple epithelial keratins involves the specific cleavage of K18, but not K8, in a
conserved non- After the initiation of the process of apoptosis in epithelial cells, but before obvious nuclear degeneration, keratin
filaments begin to reorganize, first locally and then globally throughout the cell into discrete granular structures.
Disorganized keratin filaments in frankly apoptotic cells
has been previously reported (Machiels et al., 1996 While treatment of the SNG-M epithelial cells with etoposide resulted in a dramatic change in the organization of
the K8/K18 IF into granular structures, it is not clear
whether this reorganization is due only to the phosphorylation of K18, its proteolytic cleavage, or both. One technical complication to the resolution of this question is that
epithelial cells undergoing apoptosis detach from the substrate and the degree and speed of this detachment varies
in different cell types (data not shown). Also no reagents are currently available to specifically identify cleaved K18
within the apoptotic cells that remain attached. However,
it is well known that phosphorylation is involved in the
regulation of the polymerization state of keratin filaments
during other normal cell processes (Franke et al., 1982 The decrease in K18 during the period of drug treatment is in contrast to the apparent stability of K8. While
this is consistent with the resistance of K8 to cleavage by
caspases, the fate of K8 remains to be determined. K8 is
colocalized with K18 in the keratin aggregates (data not
shown). Previous studies have shown that either K8 or
K18 that is expressed in excess of a stabilizing partner is
degraded rapidly (Kulesh et al., 1989 Caspases are considered essential to the execution of
apoptosis by a variety of stimuli. Inhibition of caspase activity, by naturally occurring inhibitors such as the bacculovirus product p35 (Bump et al., 1995 Caspase-3 can cleave K18 but not lamins A and C,
which contain the sequence VEID (Takahashi et al.,
1996
It is interesting that mutations of the V and A of the sequence VEMD/A have been detected in the K14 gene of
patients with the Weber-Cockayne form of epidermolysis
bullosa simplex (EBS), a genetic blistering skin disease
(Rugg et al., 1993 The proteolytic cleavage of K18 is of particular interest
from a clinical perspective because elevated levels of fragments of K18 are found in the serum of patients with a variety of carcinomas (Weber et al., 1984 Is the degradation of K18 of functional significance to
the apoptotic process? We expected that HR-9 cells that
express K18-D238E might be useful to explore this question. While it is clear that the expression of K18-D238E
certainly does not prevent apoptosis of mouse HR-9 cells,
a moderate effect was difficult to evaluate because of the
poor growth and mosaic expression that appears to accompany the forced expression of the mutant protein. In addition, HR-9 cells detach early in the apoptotic process. We
were thus unable to determine the impact of either K18-S53A or K18-D238E on the type of apoptotic keratin
granule organization found in SNG-M cells. A careful
comparison of the kinetics of cell death induced by different stimuli or the inducible expression of the K18-D238E
protein may help address this question in the future. K8/
K18 filaments are essential for normal development (Baribault et al., 1993). Failure of cells to undergo
appropriate apoptotic cell death is involved in a variety of
human diseases including autoimmune disease, viral infections, and cancer (Thompson, 1995
). Apoptosis also provides an orderly way to render the contents of superfluous cells benign and packaged for further degradation. The apoptosis of epithelial cells presents a significant problem of
disposal of intermediate filament cytoskeletal components,
which are highly polymerized and are relatively insoluble
in normal aqueous solutions. Packaging of the extended,
intermediate filament cytoskeleton into apoptotic bodies
is a process that is likely necessary for nearly all mammalian cells.
). Biochemical changes
include limited proteolysis of cellular proteins by the
caspase family of cysteine proteases (Alnemri et al., 1996
) that are mediators of apoptotic cell death (Martin and
Green, 1995
; Kumar and Lavin, 1996
). Genetic evidence
that supports the involvement of caspases in apoptosis includes the requirement of CED-3, a protease homologous
to the mammalian interleukin-converting enzyme (ICE)
(Yuan et al., 1993
), for cell death during development of
Caenorhabditis elegans. In mammals, ectopic expression of
members of the caspase family results in apoptosis (Miura
et al., 1993
), while expression of CrmA and p35, viral inhibitors of caspases (Ray et al., 1992
; Bump et al., 1995
),
can suppress apoptosis (Rabizadeh et al., 1993
; Gagliardini
et al., 1994
). At least 10 members of the caspase family
have been identified to date. One distinctive feature of
these proteases is the requirement of an aspartate adjacent
to the cleavage site (position P1, where P4P3P2P1/P1
indicates the position of residues relative to the cleavage
site) (Howard et al., 1991
). The cellular targets for
caspases now include over a dozen individual proteins as
diverse as transcription factors and actin (Casciola-Rosen
et al., 1994
; Lazebnik et al., 1994
; Brancolini et al., 1995
;
Emoto et al., 1995
; Cryns et al., 1996
; Janicke et al., 1996
;
Kayalar et al., 1996
; Na et al., 1996
; Song et al., 1996
; Takahashi et al., 1996
; Wang et al., 1996
; Gu et al., 1997
).
Lamins are one of the better-characterized targets for
caspases. They constitute the main structural components
of the lamina underlying the nuclear membrane. Apoptosis induced by several stimuli results in the proteolytic
cleavage of lamins to facilitate the nuclear collapse that is
characteristic of apoptosis (Rao et al., 1996
). Caspase-6
(Mch-2) appears selective for cleaving lamins A and C in
the linker region of the
helical domain after the aspartic
acid in the sequence VEID/N (Takahashi et al., 1996
). Lamin B is cleaved in the sequence VEVD/S (Rao et al.,
1996
).
). IF proteins have a large
central
helical rod domain broken by three short linker
regions and are flanked by variable nonhelical NH2 and
COOH-terminal domains. IF proteins are classified into five groups: type I, acidic keratins (K9-K20); type II, basic keratins (K1-K8); type III, vimentin, desmin, GFAP, and
peripherin; type IV, neurofilaments, nestin, and internexin; and type V, lamins. Higher eukaryotes express IF
proteins in a tissue- and differentiation-specific manner
(Steinert and Roop, 1988
). Keratins are obligate heteropolymers that constitute the IFs of epithelial cells by
the association of at least one type I and one type II keratin (Steinert et al., 1976
; Hatzfeld and Franke, 1985
). Keratin 8 (K8) and keratin 18 (K18) are the major components
of IFs of simple or single layer epithelial tissues. The expression of these proteins is generally persistent in carcinomas arising from tissues that normally express K8 and
K18 (Schaafsma et al., 1990
; Trask et al., 1990
; Schussler et
al., 1992
; Oshima et al., 1996
). The mouse form of K8 is essential for normal development; its absence results in
midgestational embryonic lethality in one genetic background and partial embryonic lethality, female sterility,
and adult colorectal hyperplasia in a different genetic
background (Baribault et al., 1993
, 1994
). Transgenic expression of a dominant acting mutant form of K18 results
in liver dysfunction (Ku et al., 1995
) and increased sensitivity to both griseofulvin and acetaminophen (Ku et al.,
1996b
). K8 and K18 have also been implicated in drug resistance (Bauman et al., 1994
; Parekh and Simpkins, 1995
;
Anderson et al., 1996
) and alterations in invasive behavior
(Chu et al., 1993
, 1996
; Pankov et al., 1997
). Both K8 and
K18 are subject to posttranslational modifications by
phosphorylation (Oshima, 1982
; Ku and Omary, 1994
; Ku
et al., 1996a
). K18 can be phosphorylated on serines by
several kinases in vitro. In vivo, the major phosphorylation site is on serine 53 (previously referred to as serine 52 relative to posttranslational processed protein) (Ku and
Omary, 1994
). Phosphorylation of K18 on serine 53 has
been implicated in increased solubility and altered polymerization (Ku and Omary, 1994
), differential localization
within hepatocytes (Liao et al., 1995a
), association with
proteins of the 14-3-3 family (Liao and Omary, 1996
), and with cellular stress (Ku et al., 1996a
). Increased phosphorylation of K18 has also been linked recently to the reorganization of hepatocyte keratin filaments subjected to the
toxin microcystin LR (Toivola et al., 1997
).
helical domains shared by all IF proteins. This cleavage may initiate the orderly processing of
the filament proteins during apoptosis in epithelial cells.
Materials and Methods
). HR-9 cells are mouse parietal endodermal cells (Chung et al.,
1977
). Cells were maintained in DME supplemented with 10% FCS and
gentamycin sulfate (30 µg/ml).
end proximal to the T7 RNA polymerase promoter (Oshima et al., 1986
). pK811 consists of the full-length
K8 cDNA cloned into the EcoRI site of pGEM1 with the 5
end proximal
to the T7 RNA polymerase promoter (Kulesh et al., 1989
). SP64B7 consists of the full-length EndoB (mouse K18) cDNA (Trevor and Oshima,
1985
; Singer et al., 1986
) cloned into the SP64 plasmid. Plasmid pGC1853
contains the human K18 gene cloned into the HindIII site of pGEM1
(Kulesh and Oshima, 1988
). pGC1853-K18S15A contains the mutation
ser53
ala (Liao et al., 1995a
) within the context of the K18 gene. This
was a very generous gift from Dr. Bishr Omary (Stanford University
School of Medicine, Stanford, CA). pCIN-K18-D238E contains the K18
cDNA with the mutation asp238
glu cloned into the EcoRI site of the pCIN4 expression vector (Rees et al., 1996
).
).
The rat monoclonal antibody TROMA1 binds EndoA but not EndoB
(Brulet et al., 1980
; Oshima, 1982
). The monoclonal antibodies, CK5 specific for K18 and M20 against K8, were purchased from Sigma Chemical
Co. (St. Louis, MO). The 3055 rabbit polyclonal antibody specific for K18
phosphorylated on serine 53 has been previously described (Liao et al.,
1995a
).
). The constructs contained
COOH-terminal His6-tags to allow for purification by affinity chromatography on Ni2+-NTA-agarose (Qiagen, Chatsworth, CA) or Pharmacia
LKB Biotech., Inc. (Piscataway, NJ) chelating Sepharose charged with
NiSO4 according to the manufacturer's instructions. The caspases were
obtained predominantly as the active two chain forms, with yields of 0.5- 1.0 mg/liter of induced culture, and were judged to be >95% pure by SDS-PAGE and NH2-terminal sequence analysis. Caspase concentrations were
determined from their observed absorbance at 280 nm and from the calculated molar extinction coefficient (Edelhoch, 1967
).
20°C). Coverslips were washed three times in PBS,
treated with PBS containing 0.1% Triton X-100 for 4 min, washed three
times with PBS, blocked with 1 mg/ml ovalbumin for 30 min, and incubated for 1 h with primary antibody (CK5 or 3055). Cells were washed
twice in PBS containing 0.1% Tween 20, twice in PBS, incubated in PBS
containing 1 mg/ml ovalbumin for 15 min, and with FITC-labeled secondary antibody for 30 min. The cells were then washed twice in PBS containing 0.1% Tween 20 and twice in PBS. To visualize DNA, coverslips were
incubated in 30 µg/ml propidium iodide for 5 min and washed once in
PBS. Coverslips were mounted over slides with Vecstashield mounting medium (Vector Laboratories, Burlingame, CA) and were visualized in
either a confocal microscope (model LSM 410; Carl Zeiss, Inc., Thornwood, NY) or a fluorescence microscope (model Axiovert 405; Carl Zeiss,
Inc.). With the confocal microscope, 12 optical sections were integrated
for each photo. Images were captured on film with the Axiovert microscope, and the negatives were scanned. Figures were printed with the use
of Adobe Photoshop software (San Jose, CA).
). 1 µg of RNA was used for translation using
a rabbit reticulocyte lysate system (Promega Corp., Madison, WI), following the instructions of the supplier with 2 µl of translation grade [35S]methionine (1,200 Ci/mmol at 10 mCi/ml) (New England Nuclear, Wilmington, DE) in a 25-µl reaction for 1 h at 30°C. Coupled transcription/ translation was performed using the TNT kit (Promega Corp.) using 2 µg
of linearized plasmid DNA in the presence of 50 µCi of radioactive methionine in a 50-µl reaction for 2 h at 30°C. Reaction products were stored
at
20°C. Truncated versions of K18 were generated from the pK187
plasmid digested with BamHI for K18-339, and ScaI for K18-269. Truncated forms of EndoB were generated from the SP64B7 plasmid digested
with AvaI for EndoB-316, ScaI for EndoB-262, and PvuII for EndoB-191.
). Cleavage products were analyzed by SDS-PAGE and fluorography.
) (12-15 h,
75 mA). Membranes were blocked by incubation in 5% nonfat dry milk
with 0.1% Tween 20 in PBS for 2 h at room temperature. After washing
four times in PBS containing 0.1% Tween 20 for 25 min, membranes were
incubated with primary antibody diluted in blocking buffer (1:5,000 for
1589, 1:2,000 for M20, and 1:500 for 3055) for 1 h at room temperature.
After washing, the membranes were incubated with secondary antibody
diluted in blocking buffer (1:7,500 horseradish peroxidase-labeled antirabbit Ig for 1589 and 3055, and 1:10,000 horseradish peroxidase-labeled antimouse Ig for M20) for 1 h at room temperature, washed again four
times in PBS containing 0.1% Tween 20 for 25 min, and detected using the
enhanced chemiluminescence system (Amersham Corp., Arlington Heights, IL) following the directions of the supplier.
). PBS-washed, floating, or
adherent cells were suspended in 0.5% Triton X-100, 10 mM EDTA, 10 mM Tris, pH 7.4, and incubated for 12-16 h at 4°C. After centrifugation at
13,000 g for 30 min, the supernatants were incubated with 20 µg/ml RNase
A for 30 min at 37°C, extracted with phenol-chloroform, and analyzed in
1.4% agarose gels in Tris-borate-EDTA buffer. In addition, the ratio of
apoptotic and normal cells in cell cultures was determined by staining the
cell nuclei with 4, 6-diamidino-2-phenylindole (DAPI). Floating cells were
harvested by centrifugation at 1,500 g for 5 min, washed with PBS, and resuspended in PBS. Adherent cells were exposed to trypsin and EDTA,
centrifuged at 1,500 g for 5 min, washed with PBS, resuspended in PBS,
and mixed with floating cells. Cells were then fixed with methanol (
20°C) for 5 min. One drop of the cell suspension was placed on a coverslip, air dried, and DAPI (2 µg/ml) was applied over the cells. Normal and
fragmented nuclei were counted using a fluorescence microscope (Dialux
20; E. Leitz, Inc., Rockleigh, NJ).
). pGC1853 and pGC1853-K18S15A were cotransfected with pPGK-Neo as a selectable plasmid at a
10:1 molar ratio. Stable transfected clones were selected by incubation
with 400 µg/ml G418. Cells with K18 expression were identified by indirect immunofluorescent staining with monoclonal antibody CK5, specific
for human K18.
Results
Fig. 1.
Induction of apoptosis in SNG-M cells
results in reorganization of K18 IF. SNG-M
cells were treated with etoposide (250 µg/ml) for 24 h and stained by indirect immunofluorescence for K18 with monoclonal antibody CK5
(A, C, and E) and with propidium iodide for
DNA (B, D, and F). Images were analyzed
with a confocal microscope. Note the granular
K18 staining associated with altered nuclear morphology.
[View Larger Version of this Image (90K GIF file)]
; Martin
et al., 1995
). In SNG-M cells treated with etoposide for 24 h,
the number of cells stained with annexin V was greater
than the number of cells with K18 granules (data not
shown). Many of the cells stained with annexin V had a
perfectly organized K18 filament network. These observations suggest alterations in the membrane, as reflected by
annexin V binding, precede K18 filament collapse and nuclei fragmentation in this order. Intermediate filament disorganization and reorganization during mitosis is associated with phosphorylation. It has been shown that one
primary phosphorylation site on K18 is serine 53 (Ku and
Omary, 1994
). To visualize K18 phosphorylated on serine 53 (P-K18), etoposide-treated SNG-M cells were stained
with antibody 3055 that specifically recognizes P-K18
(Liao et al., 1995a
). The K18 filament network was stained
with antibody 3055 very weakly in most of the nonapoptotic cells. However, a stronger reaction was observed in
some cell-cell junctions (Fig. 2 C), consistent with previous results with this antibody (Liao et al., 1995a
). Most
striking was the bright staining of the K18 granules seen in
frankly and presumptive apoptotic cells (Fig. 2, A, C, and E). Cells with only a few reactive granules but with extensive keratin filament bundles were also evident (Fig. 2 A;
Fig. 1, A, C, and E). These cells contained normal nuclei,
with no sign of fragmentation (Fig. 2 B). Other cells had
the K18 cytoskeleton completely reorganized into granules (Fig. 2, C and E). These cells contained abnormal or
fragmented nuclei. Thus, P-K18 was preferentially located
in the reorganized K18 granules of frankly and presumptive apoptotic cells and at the cell-cell contacts of normal
cells.
Fig. 2.
K18 granular structures are preferentially phosphorylated. Etoposide-treated SNG-M
cells were fixed and stained by indirect immunofluorescence for phospho-ser53 K18 with antibody 3055 (A, C, and E) and with propidium iodide for DNA (B, D, and F). Note the granular
keratin staining of some cells with normal nuclear morphology in A, the staining of cell-cell
junctions in A and C, and advanced keratin disorganization with nuclear fragmentation in C
and E.
[View Larger Version of this Image (88K GIF file)]
Fig. 3.
Treatment of
SNG-M cells with etoposide
results in specific cleavage of
K18. (A) SNG-M cells were
treated with etoposide (250 µg/ml) for 0, 12, 24, 36, 48, 60, 72, and 84 h. Adherent
and floating cells were combined, and whole-cell lysates
were analyzed by Western blot with polyclonal antibody
anti-K18 1589. This membrane was stripped and
probed consecutively with
monoclonal anti-K8 M20 (C), anti-phospho ser53-K18
antibodies (B), and monoclonal anti-K18 CK5 (D). (E)
HR-9 cells were treated with
daunomycin for 0, 12, 24, 36, 48, and 60 h. Adherent and
floating cells were combined, and whole-cell lysates
were analyzed by Western
blot with anti-K18 polyclonal
antibody, 1589.
[View Larger Version of this Image (42K GIF file)]
Fig. 4.
(A) Kinetics of induction of apoptosis in etoposide-treated SNG-M cells. In parallel experiments to the ones described in Fig. 3 A, SNG-M cells were treated with etoposide (250 µg/ml) for 0, 12, 24, 36, 48, 60, 72, and 84 h. Adherent and floating cells were combined, fixed in methanol, and stained with DAPI. Nuclei fragmentation was used as the criterion of apoptosis. (B) Comparison of the kinetics of appearance of the K18 23-kD fragment (K18 B) and its phosphorylated form (P-K18 B) by densitometric analysis of the signal detected in Western blotting shown
in Fig. 3, A and B. The y-axis represents the percentage of the
maximum signal. Maximum signal for P-K18 B was obtained after 36 h and for K18 B after 60 h. (C) Comparison of the kinetics
of disappearance of K18 and P-K18 by densitometric analysis of
the signal detected in Fig. 3, A and B.
[View Larger Version of this Image (13K GIF file)]
Fig. 5.
Different apoptotic stimuli results in the cleavage of
K18 and EndoB. (A) Western blot analysis of HR-9 (lanes 1-7)
and SNG-M (lanes 8-14) cells subjected to different apoptotic
stimuli. Adherent (A) and floating (F) cells were separately collected after etoposide (ETP) and daunomycin (DNM) treatment
and UV exposure, and whole-cell lysates were analyzed by Western blot with polyclonal anti-K18. Lanes 1 and 9 represent control untreated cells. (B) Low molecular weight genomic DNA
was analyzed from cells represented in A except for lane 1, which
contained size markers. (C) Comparison of K18 found in apoptotic cells and purified K18 cleaved in vitro by caspase-6. Cell lysates or purified protein preparation was analyzed by Western
blotting with antibody 3055. Untreated SNG-M cells (lane 1),
etoposide (ETP) treated floating SNG-M cells (lane 7), and
mouse HR-9 cells (lane 2) or apoptotic HR-9 cells (lane 6) were
compared with purified K18 (lane 4), purified K18 mixed with HR-9 cell lysate (lane 3), or K18 cleaved with caspase-6 (C6) in
vitro and mixed with HR-9 cell lysate (lane 5). Note the comigration of caspase-6-cleaved K18 (lane 5) and K18 B found in apoptotic SNG-M cells (lane 7).
[View Larger Version of this Image (46K GIF file)]
Fig. 6.
Specific cleavage in
vitro of K18, but not K8, by
caspase-6, -3, and -7. (A)
[35S]methionine-labeled K8
and K18 were incubated for
2 h at 37°C with decreasing
concentrations of caspase-6
(Cas-6) (lanes 2-10), caspase-3 (Cas-3) (lanes 11-18),
and caspase-7 (Cas-7) (lanes 19-26), and subsequently analyzed by SDS-PAGE and
fluorography. Protease was
decreased by serial 1:10 dilutions. (B) Comparison of the
proteolytic pattern of K18
and EndoB cleavage generated by caspase-6 (C6),
caspase-3 (C3), and caspase-7
(C7). A-D represent the proteolytic fragments obtained
by cleavage of K18. a-d represent the proteolytic fragments obtained by cleavage
of EndoB.
[View Larger Version of this Image (48K GIF file)]
).
Fig. 7.
Mapping of the caspase cleavage sites for K18 and EndoB. (A) Schematic representation of the deletion mutants of K18 obtained by in vitro transcription and translation of K18 cDNA digested with HindIII (K18), BamHI (K18-339), and ScaI (K18-269). (B)
[35S]methionine-labeled K18 and K18 mutants were separately incubated for 2 h at 37°C with caspase-6 (C6), caspase-3 (C3), and
caspase-7 (C7) and subsequently analyzed by SDS-PAGE and fluorography. (C) Schematic representation of the deletion mutants of
EndoB obtained by in vitro transcription and translation of EndoB cDNA digested with BamHI (EndoB), AvaI (EndoB-316), ScaI
(EndoB-262), and PvuII (EndoB-191). (D) [35S]methionine-labeled EndoB and EndoB mutants were separately incubated for 2 h at
37°C with caspase-6 (C6), caspase-3 (C3), and caspase-7 (C7) and subsequently analyzed by SDS-PAGE and fluorography.
[View Larger Version of this Image (27K GIF file)]
Fig. 8.
Site-directed mutagenesis to K18-D238E abolishes
cleavage by caspases. (A) Time-dependent cleavage of K18 and
K18-D238E with caspase-3. Equal amounts of [35S]methionine-labeled K18 and K18-D238E were incubated with caspase-3. The
reaction was stopped at 0, 15 min, 30 min, 1 h, 2 h, 4 h, and 8 h,
and samples were analyzed by SDS-PAGE and fluorography. (B)
Time-dependent cleavage of K18 and K18-D238E with caspase-6. Equal amounts of [35S]methionine-labeled K18 and K18-D238E
were incubated with caspase-6. The reaction was stopped at the
indicated times, and samples were analyzed by SDS-PAGE and
fluorography. (C) Schematic representation of the different proteolytic fragments generated by cleavage of K18 by caspases.
[View Larger Version of this Image (31K GIF file)]
helical tail domain.
ala (K18-S53A) and the asp238
glu (K18-D238E) mutant forms of
K18, and isolated clones that persistently expressed the
human protein. The human forms of K18 could be distinguished from the endogenous mouse K18 (EndoB) by the
CK5 monoclonal antibody, the 3055 antiserum, and differences in mobility in SDS-PAGE. Representative clones
with the highest proportion of CK5-positive cells were examined further.
Fig. 9.
Western blot analysis of K18, K18-D238E, and K18-S53A proteins expressed in HR-9 normal and apoptotic cells. (A)
Whole cell lysates of stably transfected HR-9 cells expressing the
indicated proteins were prepared from control cultures (C) and
after treatment with daunomycin for 24 h. Attached cells (A) and
floating cells (F) were analyzed separately. After SDS-PAGE
and transfer to nitrocellulose, the proteins were probed with the
1589 polyclonal EndoB antibody. (B) The same filter was reprobed with 3055 polyclonal antibody specific for K18 phosphorylated on ser53.
[View Larger Version of this Image (46K GIF file)]
Fig. 10.
Immunofluorescent localization of
K18, P-K18, and EndoA in HR-9 cells expressing
K18 (A and D), K18-S53A (B and E) or K18-D238E (C and F-L). Cells were stained for K18
with CK5 monoclonal antibody (A-C) and with
3055 antibody for P-K18 (D-F). Note the presence of keratin aggregates (arrows) that are preferentially phosphorylated in HR-9 K18-D238E
cells. Double staining of HR-9 K18-D238E cells with 3055 antibody (G-I) and monoclonal antibody anti-EndoA TROMA1 (J-L) show colocalization of EndoA with P-K18 in the granular structures.
[View Larger Version of this Image (116K GIF file)]
; Ku et al., 1995
). It is possible that the cleavage of K18 by caspases may play some
role in the normal metabolism of K18 filaments as well as
during apoptosis. Such an effect may only be manifest after cell division. However, it is well known that the level of
expression of dominant negative-acting intermediate filament proteins can greatly influence the appearance of the
keratin filament network (Kulesh et al., 1989
). K18-D238E was expressed from an expression vector different than
the other two forms of K18. However, we have successfully expressed K8 with a very similar expression vector in
epithelial cells without observing similar aggregates (Oshima,
R.G., and H. Baribault, unpublished observations). Thus,
we would suggest that K18-D238E behaves significantly differently than K18 or K18-S53A within mouse epithelial
cells. Cleavage of K18-D238E was inhibited in apoptotic
cells, thus confirming the importance of the site during
programmed cell death.
Discussion
helical linker region by caspase proteins.
The caspase recognition sequence in K18 is identical to
that found in lamin B and is conserved in multiple other IF family members. This suggests that the process of caspase
degradation of K18 in several types of epithelial cells may
reflect a general model of initiation of IF processing during apoptosis.
). We
have found that this reorganization preferentially involves
K18 phosphorylated on serine 53. K18 phosphorylated on
serine 53 may be a preferential substrate for proteolytic
cleavage resulting in the diagnostic 26.3-kD fragment derived from the NH2-terminal half of K18. However, the
cleavage of K18-S53A expressed in apoptotic HR-9 cells
shows that it is not strictly required. Caspases, which are
activated during apoptosis, digest K18 at the sequence
VEVD/A located within the non-
helical L1-2 linker region.
;
Klymkowsky et al., 1991
; Liao et al., 1995a
; Ku et al., 1996a
;
Toivola et al., 1997
) and of other intermediate filaments as
well (Inagaki et al., 1997
). Different stress conditions, such
as rotavirus infection and heat shock stress, can provoke
reorganization of K8/K18 IF in a process that correlates
with an increase in the phosphorylation and solubility of
K8 and K18 (Liao et al., 1995b
). Further evidence that
phosphorylation may alter the polymerization state of K8
and K18 filaments is the rapid effect of the phosphatase inhibitor, microcystin LR, on isolated hepatocytes (Toivola
et al., 1997
). In addition, these investigators detected a
protein of about the same size as the K18 B fragment in
immunoprecipitates of toxin-treated hepatocytes, which
suggests that microcystin LR may initiate a rapid apoptotic program. Furthermore, the K18-D238E mutant protein is clearly capable of being phosphorylated on serine
53 (Figs. 9 and 10). Together, the available data would be
consistent with phosphorylation of K18 being a parallel
process rather than a concerted step in a pathway leading
to cleavage.
). Furthermore the
destabilization of keratin filaments by the overexpression of truncated or mutant forms of several different keratins
results in reorganization, or collapse, of the cellular keratin filament network and the formation of granular structures (Albers and Fuchs, 1989
; Kulesh et al., 1989
; Coulombe et al., 1990
). It is possible that limited proteolytic
cleavage may participate in the reorganization of the filament networks in apoptotic epithelial cells. Cleavage of
K18 has also been reported in HeLa cells infected with adenovirus. In these cells, cleavage of the amino-terminal
end of K18 by the adenovirus L3 23-kD proteinase results
in reorganization of the K18 IF into spheroid globules
(Chen et al., 1993
).
; Xue and Horvitz,
1995
) or by synthetic inhibitors, blocks apoptosis in multiple systems (Clem et al., 1991
; Rabizadeh et al., 1993
;
Sugimoto et al., 1994
). The comigration of the apoptotic-dependent K18 B fragment with the NH2-terminal fragment of K18 generated by caspases-3, -6, and -7 is consistent with the view that a caspase is responsible for the generation of the K18 B fragment in apoptotic epithelial cells.
This is supported by the resistance of K18-D238E to cleavage in transfected apoptotic HR-9 cells. Furthermore, evidence that endogenous K18 is cleaved at the caspase recognition site during the drug-induced apoptosis of HT29
human colonic tumor cells has been obtained by another laboratory by direct protein sequencing of the K18 22- and
26-kD fragments (Bishr Omary, personal communication). All current evidence is consistent with a caspase digestion of K18 as responsible for the K18 B fragment.
While the large K18 A fragment generated by both
caspases-3 and -7 was not detected in apoptotic human
cells or mouse HR-9 cells expressing K18, this cleavage
may be masked under normal conditions by other subsequent steps in the degradation of keratin filaments. Thus,
it is not possible to deduce the identity of the caspases responsible for K18 cleavage during apoptosis based on the
cleavage products alone. However, it is of interest that all
three caspases cleave the VEVD/A sequence but that
caspase-6 is the most effective. This is consistent with the
known extended specificity of caspase-6, which prefers aliphatic side chains at the P4 position, compared with
caspases-3 and -7, which prefer acidic side chains here.
The K18 COOH-terminal site cleaved by caspases-3 and
-7 is not known but may be at DALD/S398, as this would
be better recognized by these caspases than by caspase-6. Furthermore, this cleavage site would generate a fragment
of 3.4 kD that is close to our estimated difference in size
between the C and D fragments.
). The VEVD sequence is also the cleavage site for
lamin B1 and B2 (Rao et al., 1996
). A comparison of the
L1-2 linker region of other IF proteins (Fig. 11) reveals
that the VEVD sequence is also found in K19 and K20, thus prompting the prediction that these type I keratins
would also be substrates for caspases. Furthermore, the sequence VEMD is found at the same position in K13, K14,
K15, K16, K17, desmin, and NF-L. Thus, all of the type I
keratins with the exception of K9 and K10 are potential
substrates for caspase digestion, although not necessarily
the same caspases as are active on K18. K9 and K10 are
expressed in suprabasal layers of stratified squamous epithelia in cells that are committed to terminal differentiation, which includes cell death and culminates with the
production of nucleated squames highly enriched in keratin. The absence of the caspase cleavage sites in K9 and
K10 may reflect their extracellular, structural function.
Fig. 11.
Amino acid sequences of the linker L1-2 region of the
central rod domain of the different members of the IF family. Potential caspase cleavage sites are highlighted. Sequences were obtained from Swiss-Prot database.
[View Larger Version of this Image (69K GIF file)]
; Fuchs, 1997
). Furthermore, the mutations of this region result in very subtle or no apparent differences in the polymerization of purified mutant proteins
in vitro or the appearance of keratin filaments within cells.
In this respect, K18-D238E may prove to behave in a similar fashion. This conservative change may result in subtle
structural abnormalities of K8 and K18 filaments that ultimately result in the disruption of keratin filament organization, perhaps only reflected in polymerization states of
higher order than 10-nm filaments or after cell division.
However, it is also possible that both K18-D238E and the
K14 mutations of the VEMD/A site may reflect a previously unrecognized requirement for partial caspase cleavage of type I keratin proteins in nonapoptotic cells. Some caspases, like caspase-1 (interleukin-converting enzyme),
do have functions other than in apoptosis. Furthermore,
caspase-3 activation has been demonstrated in cell processes independent of cell death (Miossec et al., 1997
).
, Bonfrer et al.,
1994
; Einarsson, 1995
). The purification of tissue polypeptide-specific antigens include a 22-kD K18 fragment (Rydlander et al., 1996
), which may correspond to the K18 C
fragment. Additional reports of the detection of K8 on the
external surfaces of some cells (Hembrough et al., 1995
) may be related to the competition between apoptotic and
necrotic death of cells, which could result in the exposure
of keratin proteins or fragments to the external milieu and
the circulation.
). However, in certain genetic backgrounds, some mice lacking K8 (and also K18 because of
degradation in organs that lack another complementary
type II keratin) survive to adulthood. These animals are
more sensitive to drugs such as griseofulvin and partial
hepatectomy and develop colorectal hyperplasia (Baribault et al., 1994
; Oshima et al., 1996
). Apoptosis may be a
common process among these different characteristics.
Furthermore, there are several reports that link K8 and
K18 expression with either drug resistance or sensitivity
(Bauman et al., 1994
; Parekh and Simpkins, 1995
; Anderson et al., 1996
). Thus, while there is as of yet no compelling evidence of the participation of K18 filaments directly
in modulating apoptosis, the recent development of mice and epithelial cell lines with keratin null alleles and various keratin mutations may provide the opportunity to determine the functional role, if any, of simple epithelial keratins in programmed cell death.
Received for publication 24 March 1997 and in revised form 11 July 1997.
Address all correspondence to Robert G. Oshima, The Burnham Institute, 10901 N. Torrey Pines Road, La Jolla, CA 92037. Tel.: (619) 646-3147. Fax: (619) 646-3193. e-mail: rgoshima{at}ljcrf.eduWe are grateful to Bishr Omary (VA Hospital and Stanford Medical School, Palo Alto, CA) for stimulating and open discussions, gifts of the 3055 polyclonal antibody, K8 and K18 proteins, the pGC1853-K18S15A plasmid, and personal communication regarding protein sequencing results of the 26- and 22-kD fragments observed in apoptotic HT-29 cells before publication.
This work was supported by grants from the National Institutes of Health, to R.G. Oshima (5 R01 CA42302) and to G. Salvesen (5 R01 HL51399), and from a Cancer Center support grant (P30 CA30199). C. Caulín was supported in part by a postdoctoral fellowship from the Spanish Ministry of Education and Science.
DAPI, 4, 6-diamidino-2-phenylindole; IF, intermediate filament; K8 and K18, keratins 8 and 18.
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