Correspondence to Janet M. Shaw: shaw{at}bioscience.utah.edu
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Abbreviations used in this paper: coIP, coimmunoprecipitation; DIC, differential interference contrast; DSP, dithiobis(sulfosuccinimidylpropionate); fis1-ts, fis1 temperature-sensitive mutation; mt-RFP, mitochondrial-targeted red fluorescent protein; pBT, bait plasmid; pTRG, target plasmid; TPR, tetratricopeptide repeat.
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Introduction |
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Mitochondrial fission has been studied extensively in the budding yeast Saccharomyces cerevisiae. Yeast mitochondrial fission requires coordinated interactions between at least three proteinsFis1p, Dnm1p, and Mdv1p. Fis1p is a tail-anchored outer membrane protein with a tetratricopeptide repeat (TPR)like domain facing the cytoplasm (Mozdy et al., 2000; Suzuki et al., 2005). Dnm1p is a large GTPase related to mammalian dynamin (Otsuga et al., 1998; Bleazard et al., 1999; Sesaki and Jensen, 1999; Fukushima et al., 2001), and Mdv1p is a WD-repeat protein with an NH2-terminal extension and a heptad repeat predicted to form a coiled coil (Fekkes et al., 2000; Tieu and Nunnari, 2000; Cerveny et al., 2001). Both Fis1p and Dnm1p have orthologues in many eukaryotic organisms, including worms (Labrousse et al., 1999; Suzuki et al., 2005), plants (Arimura and Tsutsumi, 2002; Suzuki et al., 2005), and humans (Smirnova et al., 1998; James et al., 2003; Yoon et al., 2003). To date, Mdv1p has only been found in fungi.
In yeast cells, punctate structures containing Mdv1p and Dnm1p assemble on the cytoplasmic surface of mitochondrial tubules in a Fis1p-dependent manner (Fekkes et al., 2000; Mozdy et al., 2000; Tieu and Nunnari, 2000; Cerveny et al., 2001). These structures, referred to as fission complexes, catalyze mitochondrial division. Only a subset of these complexes are associated with sites that subsequently divide, indicating that assembly and fission are temporally distinct events (Shaw and Nunnari, 2002; Legesse-Miller et al., 2003). Early events include recruitment and assembly of fission complexes, and late events presumably activate processes culminating in membrane scission.
Mdv1p, Dnm1p, and Fis1p interact with each other in vivo, and the domains required for some of these interactions have been mapped (Tieu et al., 2002; Cerveny and Jensen, 2003). However, the order of protein addition into fission complexes and the direct physical contacts required for assembly are unclear. One model suggests that Dnm1p initially assembles onto mitochondria to mark the site of fission and subsequently recruits both Mdv1p and Fis1p to form active fission complexes (Cerveny and Jensen, 2003). A variation of this model postulates that Fis1p-dependent recruitment of Dnm1p occurs first and that addition of Mdv1p remodels and activates the fission complex (Shaw and Nunnari, 2002; Tieu et al., 2002; Osteryoung and Nunnari, 2003). A third possibility is that Fis1pMdv1p interactions are a prerequisite for Dnm1p recruitment and assembly. Regardless of the accuracy of these models, Fis1p clearly plays a critical role in assembly because neither Dnm1p nor Mdv1p is recruited to mitochondria in its absence (Shaw and Nunnari, 2002).
The Fis1p cytoplasmic domain contains two distinct structural elements. Structures of mouse (Holm and Sander, 1993), human (Suzuki et al., 2003; Dohm et al., 2004), and yeast Fis1 (Suzuki et al., 2005) proteins reveal that the bulk of the cytoplasmic domain forms a highly conserved TPR-like fold with a concave surface predicted to form a binding interface (D'Andrea and Regan, 2003). The second structural element of the Fis1p cytoplasmic domain is a stretch of residues NH2-terminal to the TPR-like domain (Suzuki et al., 2003, 2005; Dohm et al., 2004), hereafter referred to as the NH2-terminal arm. In yeast, the first six residues of the NH2-terminal arm are flexible, followed by a short helix that binds the concave surface of the TPR-like domain (Suzuki et al., 2005). Multiple functions for this arm have been proposed. One model suggests that the arm facilitates Mdv1p recruitment into fission complexes, and a variation of this model proposes that the arm regulates access to the concave binding surface (Dohm et al., 2004; Suzuki et al., 2005). Both scenarios predict that the Fis1p NH2-terminal arm and TPR-like domain function independently, although this idea has not been rigorously tested.
To understand the mechanism of Fis1p function, we analyzed Fis1 proteins containing mutations in the TPR-like binding pocket or lacking the NH2-terminal arm. Our analyses show that Mdv1p binds to the concave surface of the Fis1p TPR-like domain. In support of this idea, mutations exposed at this concave surface are suppressed by mutations in Mdv1p. We provide new evidence that the Fis1pMdv1p interaction is direct and define separable functions for the Fis1p NH2-terminal arm and TPR-like domain at early and late steps during fission. In addition, our results support a revised model in which Dnm1p recruitment into functional fission complexes depends on Fis1pMdv1p interactions.
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Results |
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The fis1-3 allele was integrated at the FIS1 locus, and mitochondrial morphology was quantified in log phase cells grown at 25°C or after shifting to 37°C for 2 h. Previous studies showed that fission mutants contain a range of defective mitochondrial morphologies, including a single mitochondrion collapsed to one side of the cell, mitochondrial nets, or mitochondrial tubules lacking branches (Otsuga et al., 1998; Bleazard et al., 1999; Sesaki and Jensen, 1999). By contrast, fission-competent cells contain multiple or branched mitochondrial networks distributed at the cell cortex. In this study, we categorize the latter phenotypic group as "fission competent." When grown at 25 or 37°C, 96% of wild-type cells and 0% of fis1 cells contain fission-competent mitochondria (Fig. 1 C). fis1-3 cells, however, displayed 73% fission-competent mitochondria at 25°C versus 2% at 37°C, demonstrating that this allele causes temperature-sensitive mitochondrial morphology defects.
Fis1-3p disrupts mitochondrial fission complex assembly
Fis1-3p could affect assembly of fission complexes or later events that activate fission. To distinguish between these possibilities, we evaluated GFP-tagged Dnm1p or Mdv1p localization in fis1-3 cells grown at 25°C or shifted to 37°C for 2 h.
In wild-type cells, Dnm1-GFP in fission complexes appears as punctate structures distributed evenly along mitochondrial tubules (Fig. 2 A). At 25°C, 95% of wild-type and fis1-3 cells contain Dnm1-GFP puncta that colocalize with mitochondrial tubules. This localization pattern is maintained in FIS1 wild-type cells shifted to 37°C. Conversely, all visible Dnm1-GFP localizes to the cytoplasm in fis1-3 cells shifted to 37°C, either in rapidly moving dots or in several randomly localized structures, similar to those in fis1
cells.
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The effect of Mdv1E250Gp on fis1-3 mitochondrial morphology was compared in cells expressing either wild-type Mdv1p or Mdv1E250Gp from the inducible MET25 promoter. Although the suppressors were isolated by plate phenotype without induction of the MET25 promoter, suppression of mitochondrial morphology defects was optimal in log phase cultures after induction for 2 h in medium lacking methionine. Under these conditions, mitochondria in 70% of fis1-3 mdv1 cells incubated at 37°C and expressing Mdv1E250Gp are tubular and branched, with multiple mitochondria per cell (Fig. 4 C). By contrast, <10% of fis1-3 mdv1
cells expressing equivalent amounts of wild-type Mdv1p have fission-competent mitochondria.
Mdv1E250Gp rescues fission phenotypes in mdv1 cells and behaves like wild-type Mdv1p under all other conditions tested (Fig. S1, available at http://www.jcb.org/cgi/content/full/jcb.200506158/DC1). Thus, the effects of the Mdv1E250G suppressor protein are only apparent in fis1 mutant cells.
Mdv1E250Gp suppresses mitochondrial fission complex assembly defects in fis1-3 cells
Localization experiments confirm that fission complex assembly is restored in fis1-3 cells expressing Mdv1E250Gp. After induction of Mdv1E250Gp from the MET25 promoter in the fis1-3 mdv1 strain at 37°C, Dnm1-GFP assembles into fission complexes on mitochondria in 75% of cells (Fig. 2 B, bottom). Interestingly, overexpression of wild-type Mdv1p also allows Dnm1-GFP to assemble into punctate structures in 30% of the population at 37°C (Fig. 2 B, top). However, mitochondrial fission is not significantly rescued in these cells (Fig. 4 C). Thus, although Dnm1p can be recruited to Fis1-3pcontaining mitochondria by overexpressed Mdv1p, this Dnm1p localization is not sufficient to mediate mitochondrial fission. Instead, restoration of mitochondrial fission in fis1-3 cells requires the Mdv1E250G suppressor protein.
Analysis of GFP-Mdv1E250Gp localization in fis1-3 mdv1 cells demonstrates that the E250G substitution enhances Mdv1p recruitment to Fis1-3pcontaining mitochondria. At 25°C, GFP-Mdv1E250Gp localizes to punctate structures on mitochondria in 91% of cells, a 40% increase in localization compared with wild-type GFP-Mdv1p. After shifting to 37°C for 2 h, GFP-Mdv1E250Gp continues to localize to mitochondria in 26% of the population as compared with 3% of GFP-Mdv1pexpressing cells. These results demonstrate that GFP-Mdv1E250Gp localizes more efficiently to Fis1-3pcontaining mitochondria than GFP-Mdv1p.
Fis1-3p interaction defects are suppressed by Mdv1E250Gp
In vivo localization experiments show that Fis1-3p abolishes recruitment of both Mdv1p and Dnm1p to mitochondria at 37°C and that Mdv1E250Gp suppresses these defects. As a complementary approach, we used coimmunoprecipitation (coIP) assays to analyze the physical interaction between wild-type or mutant Fis1 proteins with either Dnm1p or Mdv1p. We also tested the effect of the Mdv1E250Gp suppressor on these interactions.
Previous attempts to coimmunoprecipitate Fis1p and Dnm1p from yeast have been unsuccessful, possibly because Dnm1p-containing structures are easily dissociated from Fis1p and mitochondria during cell solubilization. To prevent this dissociation, we spheroplasted cells expressing wild-type 9Myc-Fis1p or mutant 9MycFis1-3p and treated with a cross-linking reagent, dithiobis(sulfosuccinimidylpropionate) (DSP), before homogenization. After immunoprecipitation with anti-Myc agarose-conjugated beads, the eluted protein fractions were assayed by immunoblotting with Dnm1p-specific antibodies.
9Myc-Fis1p and 9MycFis1-3p are efficiently immunoprecipitated from DSP-treated cells (Fig. 5 A, anti-Myc, lanes 2, 4, and 6), and a fraction of cellular Dnm1p reproducibly coimmunoprecipitates with 9Myc-Fis1p (Fig. 5 A, anti-Dnm1p, lane 2). Neither Fis1p nor Dnm1p immunoprecipitates when Fis1p lacks the 9Myc tag (Fig. 5 A, anti-Dnm1p and anti-Myc, lane 8), indicating that the precipitation is antibody dependent. Significantly, Dnm1p does not coimmunoprecipitate with 9MycFis1-3p when wild-type Mdv1p is expressed (Fig. 5 A, anti-Dnm1p, lane 4), confirming that the Fis1-3p mutations disrupt Dnm1pFis1p complex formation. Expression of Mdv1E250Gp rather than Mdv1p increases the amount of Dnm1p recovered with 9MycFis1-3p (Fig. 5 A, anti-Dnm1p, lane 6), although not to wild-type levels. Together, the in vivo localization and coIP experiments demonstrate that Mdv1E250Gp partially restores Dnm1p association with Fis1-3p in mitochondrial fission complexes.
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The Mdv1 E250G substitution primarily affects interactions between Mdv1p and Fis1p
Mdv1p can interact with Fis1p, with Dnm1p, and with itself (Tieu et al., 2002; Cerveny and Jensen, 2003). The E250G substitution might stabilize fission complex assembly and suppress fis1-3 phenotypes by affecting one or more of these binding partner interactions. We were unable to purify soluble forms of Mdv1p or Mdv1E250Gp to quantitatively measure relative binding affinities in vitro. Instead, we used a two-hybrid approach to determine qualitatively which Mdv1 protein interactions are most affected by the E250G substitution.
As published previously (Tieu et al., 2002; Cerveny and Jensen, 2003), Mdv1p fused to the Gal4 activating domain (AD-Mdv1p) interacts with the Fis1p cytoplasmic domain fused to the Gal4 DNA-binding domain (BD-Fis11127p) to stimulate transcription of GAL4 reporter genes (Fig. 6 A). Although AD-Mdv1p fails to interact with the mutant BDFis1-31127p, the suppressor AD-Mdv1E250Gp interacts robustly with BDFis1-31127p. Introducing the E250G mutation does not affect interaction of Mdv1p with Dnm1p (Fig. 6 B) or with itself (Fig. 6 C) in this assay. In control experiments, constructs paired with empty vectors did not activate transcription. These results are consistent with the idea that the E250G mutation primarily affects heterotypic interactions of Mdv1p with Fis1p, rather than with Dnm1p or with itself. In combination with our coIP experiments, these studies suggest that suppression of Fis1-3p by Mdv1E250Gp is primarily the result of increased interaction between Mdv1 and Fis1 proteins.
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Western blot analysis shows that wild-type Fis1112710Hisp is extremely resistant to trypsin digestion, with uncut protein persisting at high protease concentrations (Fig. 8, top panel, top band). Digestion with increasing trypsin concentration converts this full-length protein to a stable, lower molecular weight band (Fig. 8, top panel, bottom band). Mass spectrometry of digested samples reveals a mixture of several proteolytic fragments generated predominantly by cleavage at the NH2 and COOH termini (unpublished data). NH2-terminal cleavage sites occur in the first five residues, consistent with nuclear magnetic resonance studies showing that the NH2-terminal five residues of yeast Fis1p are unstructured (Suzuki et al., 2005). COOH-terminal cleavage sites are located throughout the 10His tag used for purification. Importantly, fragments generated by cleavage at sites internal to the protein are rarely observed. Thus, the TPR-like domain and NH2-terminal helix form a highly stable fold, with the most labile portion at the extreme NH2 terminus.
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Mitochondrial fission complex assembly and fission can occur without the Fis1p NH2-terminal arm
To further define the functions of the Fis1p NH2-terminal arm and TPR-like domain, we evaluated mitochondrial morphology and fission complex assembly in cells expressing a Fis1 mutant lacking the NH2-terminal arm (Fis115155p). Fis115155p alone or Fis115155p and either Mdv1p or Mdv1E250Gp were expressed under control of the inducible MET25 promoter using a 30-min induction period, and mitochondrial morphology was quantified. Consistent with a previous study (Suzuki et al., 2005), <10% of fis1 cells expressing Fis115155p and endogenous Mdv1p have fission-competent mitochondria (Fig. 9 A). However, when both Fis115155p and Mdv1p are simultaneously induced from MET25 promoters in fis1
mdv1
cells, 45% of cells contain fission-competent mitochondria. In control experiments, 58% of fis1
mdv1
cells expressing wild-type Fis1p and Mdv1p contain fission-competent mitochondria. Interestingly, Mdv1E250Gp and Mdv1p rescue mitochondrial fission defects to the same extent in this experiment (unpublished data).
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When low levels of Fis115155p and GFP-Mdv1p are expressed in fis1 mdv1
cells, GFP-Mdv1p colocalizes with mitochondria in <10% of cells (Fig. 9 B). However, GFP-Mdv1p colocalizes with mitochondria in
55% of these cells after induction of both Fis115155p and GFP-Mdv1p. Interestingly, GFP-Mdv1E250Gp localizes to mitochondria significantly better than GFP-Mdv1p in these cells, both under low (49% localized) and high (76% localized) expression conditions. This result indicates that interactions between Mdv1p and the TPR-like domain of Fis1p are enhanced by the Mdv1 E250G substitution.
These combined observations suggest that the NH2-terminal arm facilitates, but is not strictly required for, Mdv1p recruitment or stabilization of fission complexes. Moreover, the Fis1p TPR-like domain lacking the NH2-terminal arm is sufficient to mediate fission complex assembly and fission under specific conditions.
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Discussion |
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Separable functions of the Fis1p NH2-terminal arm and TPR-like domain
In this study, we sought to relate the structure of Fis1p to its molecular role in fission. Fis1p contains three distinctive features: a COOH-terminal transmembrane domain, an NH2-terminal arm, and a six-helix TPR-like domain with a hydrophobic pocket on its concave surface. In yeast, a short helical portion of the NH2-terminal arm occupies the hydrophobic pocket. The structure of the TPR-like fold, and presumably its function, is highly conserved among orthologues (Suzuki et al., 2003, 2005; Dohm et al., 2004). However, the length, conservation, and placement of the NH2-terminal arms differ (Mozdy et al., 2000; James et al., 2003; Yoon et al., 2003; Dohm et al., 2004; Stojanovski et al., 2004), suggesting that this region has acquired a function distinct from that of the TPR-like fold.
We provide evidence that the NH2-terminal arm acts early, during complex assembly. Consistent with a previous study (Suzuki et al., 2005), removal of the Fis1p NH2-terminal arm disrupts Mdv1p and Dnm1p recruitment into fission complexes. Thus, the NH2-terminal arm is important for an early assembly step. However, in the absence of the NH2-terminal arm, fission complex assembly and fission are rescued by overexpression of Mdv1p. Therefore, the NH2-terminal arm is not essential after assembly, and the TPR-like domain is sufficient for fission events once assembly has occurred.
Characterization of the Fis1pMdv1p interaction
To date, all experiments evaluating Fis1pMdv1p interactions have been performed in live cells (yeast two-hybrid assay and genetic studies) or extracts (coIP) containing other yeast proteins. Here we use an Escherichia coli two-hybrid assay to demonstrate that Fis1p and Mdv1p interact directly in the absence of other yeast proteins.
Is the Mdv1p-binding site on yeast Fis1p formed by the NH2-terminal arm, the TPR-like domain, or both? It has been hypothesized that the TPR-like hydrophobic pocket is a binding surface for other fission complex proteins (Dohm et al., 2004; Suzuki et al., 2005). Three lines of evidence that are presented in this study support the idea that Mdv1p occupies the Fis1p TPR-like binding pocket, possibly by displacing the NH2-terminal arm (Fig. 10). First, mutations in the Fis1p TPR-like binding pocket are suppressed by mutations in Mdv1p. Second, overexpressed Mdv1p interacts with Fis115155p, which lacks the NH2-terminal arm. Third, the Mdv1E250Gp suppressor interacts with Fis115155p, even when expressed at low levels. Thus, the TPR-like domain alone interacts with Mdv1p, and this interaction is augmented by the Mdv1p E250G substitution. Although these studies establish that Mdv1p interacts with the TPR-like domain, the Fis1p NH2-terminal arm is clearly important. We propose that the Fis1p NH2-terminal arm facilitates recruitment and/or stabilization of Mdv1p during fission complex assembly.
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Three different assays indicate that fission complex stabilization results from increased affinity of Mdv1E250Gp for Fis1-3p. First, GFP-Mdv1E250Gp localizes to Fis1-3pcontaining mitochondria more efficiently than wild-type GFP-Mdv1p. Second, significantly more Mdv1E250Gp coimmunoprecipitates with Fis1p and Fis1-3p compared with wild-type Mdv1p. Third, Mdv1p interaction with Fis1-3p is restored by the Mdv1p E250G mutation in yeast two-hybrid assays. Importantly, the Mdv1E250G suppressor protein primarily affects Mdv1pFis1p interactions; in two-hybrid assays, Mdv1pMdv1p and Mdv1pDnm1p interactions are unaffected by this suppressor mutation.
Despite its increased interaction with both wild-type Fis1p and Fis1-3p (Fig. 5 B), Mdv1E250Gp does not cause fission defects in cells expressing wild-type Fis1p. Thus, enhanced binding between Mdv1p and Fis1p does not appear to interfere with downstream events in fission. One possible interpretation of this result is that dissociation of Mdv1p from Fis1p is not required for late steps in fission.
Although the Mdv1E250Gp suppressor increases interactions with Fis1-3p, the suppression is not allele specific. Mdv1E250Gp also rescues other conditional fis1 alleles containing TPR domain mutations (unpublished data). Moreover, two additional MDV1 alleles containing mutations in or near the predicted coiled coil suppress fission defects in fis1-3 (unpublished data). We propose that different TPR-like binding pocket mutations in Fis1p cause similar structural changes and that these changes are suppressed by a novel class of MDV1 alleles with mutations in the predicted coiled coil.
Previous work showed that amino acids NH2-terminal to the Mdv1p coiled coil are sufficient for Fis1p interactions (Tieu et al., 2002). This study also suggested that the coiled-coil region mediates Mdv1p self-interactions. Our studies suggest that Mdv1p suppressor mutations in the predicted coiled-coil region affect Fis1p, but not Mdv1p, interactions. Clearly, the mechanisms regulating Mdv1pFis1p interactions in vivo are more complicated than previously appreciated. One possibility is that the Mdv1p coiled coil regulates binding of the Mdv1p NH2-terminal region to the Fis1p TPR-like domain.
A revised model for assembly of mitochondrial fission complexes
Current models propose that Dnm1p assembles into mitochondrial fission complexes, and Mdv1p recruitment into these complexes activates a rate-limiting step in the fission pathway (Shaw and Nunnari, 2002; Cerveny and Jensen, 2003; Osteryoung and Nunnari, 2003). In our study, Dnm1p assembly defects in fis1 mutants are rescued by restoring Fis1p interactions with either Mdv1p or Mdv1E250Gp. Based on these findings, we favor the idea that direct association of Mdv1p with Fis1p precedes and facilitates Dnm1p recruitment (Fig. 10). According to this revised model, Mdv1p recruitment into fission complexes cannot be rate limiting.
Several other observations are consistent with the idea that Dnm1p assembles onto preexisting Fis1pMdv1p complexes. GFP-Mdv1p is constitutively and uniformly bound to Fis1p on the outer membrane as well as in fission complexes with Dnm1p (unpublished data). Thus, it is likely that Dnm1p assembles from the cytoplasm onto Fis1pMdv1p complexes. Although Dnm1p assembles into nonfunctional fission complexes in mdv1-null cells (Fekkes et al., 2000; Mozdy et al., 2000; Tieu and Nunnari, 2000; Cerveny et al., 2001), we observed that the number, distribution, and size of Dnm1p complexes differ slightly from those in MDV1 wild-type cells (unpublished data). The latter observation is explained by a recent study showing that Mdv1p and its paralogue Caf4p play redundant roles in Dnm1p recruitment and assembly (Griffin et al., 2005), although only Mdv1p is essential for fission. In the future, it will be important to understand how Dnm1p and Mdv1p are reorganized after assembly to form the functional fission complexes observed in vivo.
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Materials and methods |
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To construct pET24a + fis1 112710HIS and pET24a + fis1-3112710HIS, fis1 fragments were PCR amplified with a 3' 10HIS tag encoded by the reverse PCR primer and ligated into NdeISalIdigested pET24a(+). To construct pBT + fis11127, a fis11127 PCR fragment flanked by EcoRIXhoI sites was cloned into pBT to create an in-frame fusion of FIS11127 with the lambda-cI gene of the pBT vector backbone. For pTRG + MDV1, an MDV1 PCR fragment flanked by EcoRIXhoI sites was first cloned into pBT and then subcloned into pTRG using NotIXhoI sites to create an in-frame fusion with the RNAP gene of the pTRG vector backbone. All inserts used for yeast two-hybrid assays were PCR amplified and cloned into BamH1SalIdigested pAD-C1 and pBD-C1 (James et al., 1996). To construct pRS415MET25 + 9MYC-FIS1 and pRS415MET25 + 9MYCfis1-3, PCR-amplified fragments flanked by HindIIIXhoI sites were cloned into pRS415MET25-9MYC (Mozdy et al., 2000). For pRS416MET25 + MDV1 and pRS416MET25 + mdv1E250G, pRS415MET25 + fis115155, PCR fragments were cloned into BamHISalIdigested vector backbones. pRS415MET25 + GFP-mdv1E250G was created by site-directed mutagenesis (Stratagene) using pRS415MET25 + GFP-MDV1 as a template. To construct pRS415MET25 + GFP-MDV1 and pRS416MET25 + GFP-MDV1, MDV1 PCR fragments flanked by SalIXhoI sites were cloned into pRS415MET25 + GFP or pRS416MET25 + GFP plasmids.
Selection and integration of fis1-ts alleles
Mutations introduced into the FIS1 ORF by PCR amplification with Taq DNA polymerase were ligated into linearized pRS416 by in vivo gap repair (Orr-Weaver et al., 1983) in fis1 fzo1
cells. Cells containing fis1-ts plasmids were identified by their ability to grow on glycerol at 37°C, but not 25°C. After recovery and phenotypic verification, plasmids were sequenced to identify mutations in the FIS1 ORF. Mutations in alleles with multiple amino acid changes were separated by site-directed mutagenesis, and only mutations contributing to the phenotype were analyzed further. fis1-ts alleles were integrated into the FIS1 locus using a two-step replacement scheme. First, the FIS1 ORF was replaced with URA3 by standard homologous recombination techniques (Rothstein, 1991). Second, URA3 was replaced with a fis1-ts allele by homologous recombination, an event detected by selection on medium containing 5FOA (Boeke et al., 1987). Putative fis1-ts integrants were screened by PCR and confirmed by DNA sequencing.
Screen for mdv1 suppressors of fis1-ts alleles
mdv1 suppressors of fis1-ts alleles were generated by PCR mutagenesis and gap repair as described for selection of fis1-ts alleles, except that mutations were introduced into the MDV1 ORF by gap repair with linearized pRS415-MET25 in the fis1-ts fzo1-1 strain. fis1-ts fzo1-1 cells containing mdv1 suppressor mutations on plasmids were no longer able to grow on glycerol at 37°C.
Bacterial and yeast two-hybrid analyses
Yeast two-hybrid studies were performed in the Y187 S. cerevisiae strain background (CLONTECH Laboratories, Inc.) via a filter-lift assay as described previously (Guthrie and Fink, 2002).
The E. coli BacterioMatch II Two Hybrid System (Stratagene) was used to evaluate direct interactions of proteins expressed from the pBT + fis11127 and pTRG + MDV1 plasmids. Interaction between proteins was assayed by spotting serial dilutions (1:5) of cells on M9 histidine + 8 µg/ml tetracycline, 25 µg/ml chloramphenicol, and 0.05 mM IPTG in the presence and absence of 5 mM 3AT (Sigma-Aldrich) and monitoring growth at 30°C after 2 d.
Fluorescence microscopy and quantification of mitochondrial phenotypes
Mitochondrial morphology was scored in wild-type and mutant cells expressing fast-folding mitochondrial-targeted red fluorescent protein (mt-RFP; provided by B. Glick, University of Chicago, Chicago, IL; Bevis and Glick, 2002) from pYX142 (Novagen), pRS414-GPD (Mumberg et al., 1995), or pRS416-GPD (Mumberg et al., 1995) plasmids. Dnm1-GFP localization was scored in strains expressing both Dnm1p-rsGFP (Mozdy et al., 2000) from pRS414 or pRS416 and mt-RFP. GFP-Mdv1p localization was scored in strains expressing both GFP-Mdv1p from pRS415-MET25 or pRS416MET25 and mt-RFP. Basal levels of GFP-Mdv1p transcription from the MET25 promoter without induction produced sufficient GFP-Mdv1p (a two- to fivefold increase over endogenous Mdv1p levels) for visualization with no adverse affects on mitochondrial morphology or fission protein localization.
Before quantification, strains were grown overnight at 25°C, diluted to 0.5 OD600, split into 37 and 25°C cultures, and grown another 23 h into log phase. Strains overexpressing genes from the MET25 promoter were induced for either 2 h or 30 min. For 2-h inductions, strains were diluted to 0.5 OD600 in medium lacking methionine. Strains induced for 30 min were grown for 1.5 h after dilution, resuspended in medium lacking methionine, and grown another 30 min into log phase. In log phase cultures under repressed conditions, the MET25 promoter is leaky and produces two- to fivefold more Mdv1p or Mdv1E250Gp than the endogenous MDV1 promoter. After induction for 30 min to 2 h, production of Mdv1p or Mdv1E250Gp by the MET25 promoter increased to 50-fold endogenous levels.
Cells were visualized using a microscope (Axioplan 2 Imaging; Carl Zeiss MicroImaging, Inc.) equipped with differential interference contrast (DIC) optics, epifluorescence capabilities, and a Plan-Apochromat x 100 objective (NA 1.4; Carl Zeiss MicroImaging, Inc.). Filter sets (excitation/beamsplitter/emission) for GFP and red fluorescent protein fluorescence were BP 470 ± 20/FT 495/LP 525 ± 25 and BP 546 ± 6/FT 560/BP 575640, respectively. Images were captured and deconvolved using a monochrome digital camera (AxioCam; Carl Zeiss MicroImaging, Inc.) and appropriate software (AxioVision 3.1; Carl Zeiss MicroImaging, Inc.) and were assembled using Photoshop (Adobe Systems).
Protein purification and limited proteolysis
BL21(DE3) E. coli cells (Novagen) containing either pET24a + fis11127 10HIS or pET24a + fis1-3112710HIS plasmids were grown in LB medium plus 30 µg/ml of kanamycin at 37°C to mid-log phase and induced with 1 mM IPTG for 24 h. Cells expressing Fis1112710Hisp were induced at 37°C, and cells expressing Fis1-3112710Hisp were induced at RT. Wild-type and mutant Fis1 fusion proteins were purified from cleared cell lysates on Ni2+ affinity column chromatography as described by the manufacturer (Novagen).
Limited proteolysis of purified Fis1112710Hisp and Fis1-3112710Hisp proteins was performed by incubating 1 or 1.8 µg, respectively, in buffer (100 mM NaCl, 20 mM sodium phosphate, pH 7.0, 1 mM DTT, and 100 µM EDTA) with either 0, 0.1, 0.5, 1.0, 2.0, 3.0, 4.0, or 5.0 µg of trypsin (Sigma-Aldrich) in a 10-µl reaction volume. Reactions were prepared on ice, incubated for 2 min at 37°C, and stopped by boiling in 5x SDS-PAGE sample buffer. Samples were analyzed by SDS-PAGE and Western blotting using anti-Fis1p antibodies (Mozdy et al., 2000). For mass spectrometry analysis, purified proteins were dialyzed in buffer (150 mM NaCl, 20 mM sodium phosphate buffer, pH 7.0, and 25 mM DTT), and 9.5 µM of either Fis1112710Hisp or Fis1-3112710Hisp was digested with 4 µg of trypsin for 2 min at 37°C. Reactions were stopped with 1% trifluoroacetic acid at RT and then frozen at 80°C until analysis. Samples were analyzed by electrospray ionization at the University of Utah Mass Spectrometry Facility.
CoIP assays
CoIP assays were performed using mdv1 strains expressing Fis1p, 9Myc-Fis1p, or 9MycFis1-3p from the genomic locus and either pRS416-MET25-MDV1 or pRS416-MET25-mdv1E250G from a plasmid. For Dnm1pFis1p coIPs with DSP cross-linking, strains were grown at 25°C in synthetic dextrose medium to early log phase, washed, resuspended in medium lacking methionine, and grown at 37°C for another hour. A total of 35 OD600 units of cells were collected. Cells were treated as described previously for mitochondrial preparation (Kondo-Okamoto et al., 2003) with the following modifications. After Zymolyase treatment for 45 min at 37°C, cross-linking was performed with 2.5 mM DSP (Pierce Chemical Co.) for an additional 30 min, and DSP was quenched by addition of 50 mM glycine (also present in all subsequent buffers). After homogenization, samples were used directly for coIP without mitochondrial isolation. Homogenized samples were solubilized for 15 min in IP buffer (1% Triton X-100, 150 mM NaCl, 30 mM Hepes-KOH, pH 7.4, and 1:500 protease inhibitor cocktail [Calbiochem]). After centrifugation at 12,500 g for 10 min, the supernatant was incubated with 40 µl antic-Myc agarose-conjugated beads (Sigma-Aldrich) for 1 h at 4°C. Agarose beads were collected, washed in IP buffer, and incubated in 50 µl SDS-PAGE sample buffer lacking ß-mercaptoethanol at 60°C for 8 min to release bound proteins. After addition of ß-mercaptoethanol and boiling, samples were analyzed by SDS-PAGE and Western blotting with anti-Myc (Santa Cruz Biotechnology, Inc.), affinity-purified anti-Dnm1p (Otsuga et al., 1998), and anti-porin (Invitrogen) antibodies. The load was 0.7 OD600 cell equivalents per lane, and the elution was 34 OD600 cell equivalents per lane.
For coIP without DSP cross-linking, cells were grown overnight at 25°C in S-dextrose lacking leucine and diluted to 0.5 OD600 in S-dextrose lacking leucine and methionine at 37°C, and 70 OD600 cells were harvested. CoIP was performed as described previously (Fukushima et al., 2001) with the following changes: lysis was performed in IP buffer (0.5% Triton, 150 mM NaCl, 1 mM EDTA, 50 mM Tris, pH 7.4, and 1:500 protease inhibitor cocktail set III [Calbiochem]), and soluble proteins were precipitated with anti-Myc beads at 4°C for 2 h. Proteins were eluted and analyzed as described in the previous paragraph, decorating with anti-Mdv1p (provided by J. Nunnari, University of California, Davis, Davis, CA), anti-Myc, and anti-porin antibodies. The load was 1.25 OD600 cell equivalents per lane, and the elution was 21 OD600 cell equivalents per lane.
Online supplemental material
Fig. S1 shows that Mdv1E250Gp behaves like wild-type Mdv1p in the presence of wild-type Fis1p. Online supplemental material is available at http://www.jcb.org/cgi/content/full/jcb.200506158/DC1.
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Acknowledgments |
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This work was funded by a National Institutes of Health (NIH) grant awarded to J.M. Shaw (GM53466), an NIH training grant awarded to E.M. Coonrod (5 T32 DK007115), and a University of Utah Bioscience Undergraduate Research Program grant awarded to T.K. Anderson. The Utah Health Sciences DNA/Peptide and Sequencing Facilities are supported by a National Cancer Institute grant (5-P30CA42014).
Submitted: 24 June 2005
Accepted: 19 September 2005
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