Correspondence to Peter Mundel: peter.mundel{at}mssm.edu
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Introduction |
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Nuclear import is regulated at numerous levels, including the posttranslational modification of cargo proteins. Phosphorylation of some cargoes can mask their nuclear localization signal (NLS), thereby abrogating their interaction with the cytoplasmic import receptor, importin , and blocking nuclear import. Other cargo proteins show enhanced binding to importin
after phosphorylation, resulting in an increased nuclear import rate (Jans et al., 2000). The mechanism underlying the phosphorylation-dependent nuclear import of some NLS-containing cargo proteins remains elusive. Phosphorylation may directly modulate the affinity of the NLS for importin
. Alternatively, it may cause conformational changes within the cargo or alter its binding to another protein, both of which may reveal or mask the NLS (Harreman et al., 2004).
Myopodin is a dual-compartment, actin-bundling protein that is found in the nucleus of undifferentiated myoblasts and at the Z-disc of differentiated myotubes, but it shuttles back to the nucleus during thermal stress (Weins et al., 2001). In normal bladder epithelium, myopodin is localized in the cytoplasm and in the nucleus (Sanchez-Carbayo et al., 2003). Of note, the loss of nuclear myopodin expression can predict the clinical outcome of human progressive bladder cancer, implying that myopodin functions as a tumor suppressor (Sanchez-Carbayo et al., 2003). The mechanism that regulates the subcellular localization of myopodin is unknown. We describe a novel mechanism for regulating the nuclear import of an NLS-containing protein. We show that the phosphorylation-dependent binding of 14-3-3 to myopodin is required for the interaction of myopodin with importin and the subsequent nuclear import of myopodin. We discuss 14-3-3 binding as a novel, regulatory step that promotes the nuclear import of cargo proteins.
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Results |
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Endogenous myopodin specifically interacts with 14-3-3ß in myocytes
Myopodin specifically interacts with 14-3-3ß in GST pull-down assays. GST14-3-3ß, but not GST alone, specifically bound to myopodin from adult mouse skeletal muscle and C2C12 myoblast extracts (Fig. 1 E). To further confirm the interaction, endogenous proteins were immunoprecipitated from mouse heart extracts using an anti14-3-3ß antibody and the myopodin-specific antibody SRIB2. Anti14-3-3 precipitated 14-3-3ß from the extract and also coprecipitated myopodin (Fig. 1 F, left). Conversely, antimyopodin coimmunoprecipitated 14-3-3ß (Fig. 1 F, right). No interaction was found with a control IgG.
Myopodin interacts with all 14-3-3 isoforms except 14-3-3
Mammals express seven different 14-3-3 isoforms (ß, ,
,
,
,
, and
; Fu et al., 2000), but the yeast two-hybrid screen had identified only the ß isoform as a myopodin-interacting protein. The binding of myopodin to other 14-3-3 isoforms was tested in pull-down studies of GST14-3-3 fusion proteins and purified FLAG-myopodin (Fig. 1 G). Myopodin strongly bound 14-3-3ß,
,
, and
(Fig. 1 G). The interaction with 14-3-3
and
was weaker, and no binding was found for 14-3-3
(Fig. 1 G). The K49E substitution in the binding groove of 14-3-3 causes the loss of target binding (Zhang et al., 1997), and a 14-3-3
K49E mutant also failed to bind to myopodin (Fig. 1 G), confirming the specificity of the interaction. 14-3-3 dimerization is crucial for target protein binding and 14-3-3 functionality (Tzivion et al., 2001). Therefore, the requirement of 14-3-3 dimerization for myopodin binding was tested with a 14-3-3ß construct (14-3-3ß
N) that lacked the NH2-terminal dimerization domain (Seimiya et al., 2000). 14-3-3ß
N also bound to FLAG-myopodin, but to a lesser extent than wild-type 14-3-3ß (Fig. 1 G).
Two consensus 14-3-3 motifs mediate the binding of myopodin to 14-3-3ß
Myopodin contains two consensus 14-3-3binding motifs (Fig. 1 H, top) that can mediate phosphorylation-dependent 14-3-3 binding (Yaffe et al., 1997). To map the 14-3-3ßbinding sites in myopodin, both motifs were deleted separately (14-3-3#1 and
14-3-3#2) or together (
14-3-3#1 + 2; Fig. 1 H). Full-length myopodin and deletion constructs were coexpressed with GFP14-3-3ß in HEK-293 cells and were analyzed by coimmunoprecipitation. FLAG full-length myopodin showed a strong interaction with GFP14-3-3ß, whereas a weak binding was found for FLAG
14-3-3#1 and FLAG
14-3-3#2. No binding to 14-3-3ß was found after the deletion of both motifs (FLAG
14-3-3#1 + 2). Hence, myopodin contains two functional 14-3-3binding sites that mediate the interaction between myopodin and 14-3-3ß.
14-3-3 function is required for the nuclear import of myopodin
14-3-3 proteins can regulate the subcellular localization of binding partners (Muslin and Xing, 2000), and myopodin is a dual-compartment protein (Weins et al., 2001). This raises the possibility that 14-3-3ß participates in regulating the subcellular localization of myopodin. A dominant negative mutant of 14-3-3 that lacks the NH2-terminal dimerization domain (14-3-3
N) redistributes the catalytic subunit of human telomerase from the nucleus to the cytoplasm (Seimiya et al., 2000). Therefore, we tested the effect of 14-3-3ß
N (Fig. 1 G) on the subcellular localization of myopodin in myoblasts. In cells expressing GFP14-3-3ß
N, myopodin showed a cytoplasmic localization (Fig. 2 A, bottom left), whereas in cells expressing full-length GFP14-3-3ß (Fig. 2 A, top left) or GFP alone (not depicted), myopodin was localized in the nucleus.
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Two NLSs and two 14-3-3binding motifs are required for the nuclear import of myopodin
Myopodin contains two putative nuclear localization sequences: NLS#1 and NLS#2 (Fig. 1 A). To assess the functionality of these NLSs, we deleted them separately (NLS#1 and
NLS#2) or in combination (
NLS#1 + 2), and the subcellular distribution was analyzed by confocal microscopy (Fig. 2 B). In addition, myopodin-induced F-actin filaments (Weins et al., 2001) were visualized with rhodamine-labeled phalloidin, and nuclei were visualized with DAPI. GFP full-length myopodin was mainly detected in the nucleus (Fig. 2 B). The quantitative analysis showed that 73.4% of GFP full-length myopodin was found in the nucleus, compared with 14.8% for
NLS#1 and 13.4% for
NLS#2 (Fig. 2 C). The simultaneous deletion of both NLSs (
NLS#1 + 2) did not further decrease the amount of nuclear myopodin (14.2%; Fig. 2 C). The differences between full-length myopodin and
NLS#1,
NLS #2, and
NLS #1 + 2 were significant (P < 0.01; t test). Hence, both NLSs of myopodin are functional and necessary for an efficient nuclear import of myopodin. However, even in the absence of both NLSs,
14% of total GFP fusion protein is still found in the nucleus, suggesting that other domains are also involved in the nuclear import of myopodin. To determine whether the observed nuclear exclusion of myopodin in the presence of 14-3-3ß
N (Fig. 2 A) resulted from a functional loss of the 14-3-3ßmyopodin interaction, we next expressed myopodin deletion constructs that lacked the identified 14-3-3binding motifs as GFP fusion proteins in C2C12 myoblasts (Fig. 2 B). The deletion of either one (
14-3-3#1 and
14-3-3#2) or both (
14-3-3#1 + 2) 14-3-3binding motifs resulted in a dramatic decrease of nuclear myopodin. 20% of
14-3-3#1, 21.8% of
14-3-3#2, and 19.1% of
14-3-3#1 + 2 were localized in the nucleus (Fig. 2 C), which is a significant difference from the 73.4% for full-length myopodin (P < 0.01; t test). Next, we examined whether 14-3-3ß can act synergistically with NLSs to maximize the efficacy of nuclear import. Therefore, we created a GFPmyopodin construct that lacked both 14-3-3binding motifs and both NLSs (Fig. 2 B,
14-3-3#1 + 2 +
NLS#1 + 2). GFPmyopodin
14-3-3#1 + 2 +
NLS#1 + 2 showed 6.4% nuclear localization (Fig. 2 C), which was significantly less when compared with
NLS#1 + 2 or
14-3-3#1 + 2 (P < 0.01; t test). Together, both NLSs and both 14-3-3binding sites are required for the efficient nuclear import of myopodin.
The two 14-3-3 motifs are necessary for the binding of recombinant myopodin to endogenous 14-3-3ß in myoblasts
To determine whether the various myopodin mutant forms (Fig. 2 C) can bind to endogenous 14-3-3ß, FLAG-tagged proteins were expressed in C2C12 myoblasts and were immunoprecipitated with anti-FLAG antibody (Fig. 3 A, bottom). Similar to full-length myopodin (FLAG-full-length), FLAG14-3-3#1 and FLAG-
14-3-3#2 coprecipitated endogenous 14-3-3ß, albeit to a lesser extent (Fig. 3 A, top). In contrast, FLAG-
14-3-3#1 + 2 did not bind to myopodin. These results corroborate the interaction studies in transfected HEK-293 cells (Fig. 1 H) and suggest that binding to 14-3-3ß is required for the nuclear import of myopodin. The deletion of the two NLSs separately (FLAG-
NLS#1 and FLAG-
NLS#2) or together (FLAG-
NLS#1 + 2) did not abrogate the myopodin14-3-3ß interaction. Therefore, the two NLSs in myopodin are not necessary for its binding to 14-3-3ß. As expected, FLAG
14-3-3#1 + 2 +
NLS#1 + 2 did not interact with endogenous 14-3-3ß (Fig. 3 A).
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Inhibition of 14-3-3 binding with a high-affinity peptide antagonist abrogates the interaction between myopodin and importin
To further confirm this finding with another approach, the experiment was repeated with full-length myopodin in the absence or presence of the R18 peptide, a well-established, high-affinity competitive inhibitor of 14-3-3 binding (Wang et al., 1999). R18 abolished the interaction between FLAG-myopodin and endogenous 14-3-3ß and between the binding of myopodin and importin (Fig. 3 C). This result lends further support to our hypothesis that the binding of myopodin to importin
critically depends on the interaction of myopodin with 14-3-3.
The direct binding of myopodin to importin requires the presence of 14-3-3
To determine whether myopodin can directly bind to importin , we conducted in vitro reconstitution studies with purified importin
, myopodin, and 14-3-3ß. To do so, purified GST-tagged importin
was incubated with purified, FLAG-tagged myopodin, FLAG-tagged 14-3-3ß, or both together and analyzed by GST pull-down studies (Fig. 3 D). When importin
was incubated with myopodin (Fig. 3 D, left) or 14-3-3ß (Fig. 3 D, middle) alone, no interaction was found. In contrast, when all three proteins were present, both myopodin and 14-3-3ß could be eluted together with importin
, but not with GST alone (Fig. 3 D, right). These results confirm that 14-3-3 cannot directly bind to importin
, as expected from the absence of a functional NLS (Brunet et al., 2002). Furthermore, the direct binding of purified myopodin to importin
only occurs in the presence of 14-3-3. Finally, these results demonstrate that the interaction between 14-3-3ß and importin
is indirect and is mediated by myopodin.
Myopodin is phosphorylated in vivo
14-3-3 proteins are phosphoserine/threonine-binding proteins (Muslin et al., 1996), and many 14-3-3 interactions depend on the phosphorylation of the target protein (Yaffe, 2002). Therefore, we examined whether the myopodin14-3-3 interaction is regulated by phosphorylation. To this end, purified FLAG-myopodin was dephosphorylated with protein phosphatase (
-PPase), which reduced the molecular size of myopodin (Fig. 4 A). Moreover,
-PPase treatment converted the fuzzier signal of the phosphorylated protein into a more compact and sharper band (Fig. 4 A, top). These findings show that myopodin is phosphorylated in vivo.
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Dephosphorylation of myopodin abrogates importin binding
To further determine whether the phosphorylation of myopodin is not only required for 14-3-3 binding but also for importin binding, FLAG-tagged myopodin was treated with
-PPase and was incubated with X. laevis protein extract (Fig. 4 D). In the absence of
-PPase treatment, FLAG-myopodin bound to 14-3-3ß and importin
(Fig. 4 D, left). In contrast, the dephosphorylation of myopodin with
-PPase abrogated the interaction with 14-3-3ß and importin
(Fig. 4 D, right). These findings further support the hypothesis that the interaction of myopodin with importin
requires the phosphorylation-dependent binding of myopodin to 14-3-3.
S225 and T272 mediate the phosphorylation-dependent binding of myopodin to 14-3-3ß
The removal of the 14-3-3binding sites (Fig. 1 H and Fig. 3, A and B) and the dephosphorylation of myopodin (Fig. 4, BD) abrogate 14-3-3 binding, suggesting that the amino acids mediating the phosphorylation-dependent interaction are localized within the two 14-3-3binding motifs. According to the consensus 14-3-3binding motifs RSxpS/TxP (mode 1) and RxxxpS/TxP (mode 2; Yaffe et al., 1997), we predicted that S225 and T272 were phosphoacceptor sites (Fig. 4 E). To test this hypothesis, we mutated these amino acids to alanine (S225A and T272A), which cannot be phosphorylated by protein kinases. As a control, S273, which was not expected to be crucial for 14-3-3 binding, was also mutated (S273A; Fig. 4 E). Myopodin and its point mutation variants were expressed as FLAG fusion proteins in HEK-293 cells and were purified and incubated with immobilized GST14-3-3ß. Wild-type myopodin could bind GST14-3-3ß, whereas no interaction was found for S225A, T272A, or S225AT272A (Fig. 4 F). In contrast, S273A showed normal 14-3-3 binding. As expected from the single mutation S225A, the combined removal of serine 225 and serine 273 (S225AS273A) also resulted in the loss of 14-3-3 binding (Fig. 4 F). Together, S225 and T272, but not S273, are crucial for the interaction of myopodin with 14-3-3ß. It also confirms the finding that the two 14-3-3binding motifs in myopodin are necessary and sufficient for the efficient interaction with 14-3-3ß (Fig. 1 H). To determine whether the phosphorylation of S225 and T272 is required for 14-3-3 binding, the phosphorylation of both residues was mimicked by the substitution of negatively charged amino acids. S225 was replaced by aspartic acid (S225D) and T272 by glutamic acid (T272E; Fig. 4 G). The single (S225D and T272E) or combined substitutions (S225DT272E) did not alter binding to GST14-3-3ß (Fig. 4 G, top). In contrast, after dephosphorylation with -PPase and before GST14-3-3ß binding (Fig. 4 G, bottom), the binding of wild-type myopodin was abrogated, whereas the binding of S225D and T272E was dramatically reduced. In contrast, S225DT272E retained strong binding to 14-3-3ß, demonstrating that the phosphorylation of S225 and S272 is necessary for the efficient binding of myopodin to 14-3-3ß.
S225 and T272 mediate the phosphorylation-dependent nuclear import of myopodin
To determine whether the aa S225 and T272 of myopodin are not only required for 14-3-3 binding but are also required for the nuclear import of myopodin, the subcellular distribution of GFP-tagged wild-type and mutant myopodin was analyzed by confocal microscopy (Fig. 5 A) and was quantified as described above (Fig. 2 C). As shown previously (Fig. 2 B; Weins et al., 2001), wild-type myopodin was mainly localized in the nucleus (Fig. 5 A). The quantitative analysis showed 77.4% nuclear localization (Fig. 5 B). The removal of single phosphorylation sites (S225A and T272A) resulted in a significantly decreased nuclear localization (20.1% for S225A and 20.5% for T272A; Fig. 5 B) versus 77.4% for wild-type myopodin (P < 0.01; t test). The combined replacement of S225 and T272 (Fig. 5 A, S225AT272A) further decreased nuclear myopodin to 10.5% (Fig. 5 B), a significant difference from the single mutations S225A and T272A (P < 0.01; t test). In contrast, S273A showed normal nuclear localization (78.8% nuclear; Fig. 5 B). The active mutants S225D, T272E, or S273D showed 75.6%, 80.3%, and 75.7% nuclear localization, respectively. The highest percentage of nuclear myopodin was found for S225DT272E (Fig. 5 A). However, the difference to wild type (83.5% for S225DT272E versus 77.4% for wild-type myopodin) was not significant. Altogether, these experiments show that the phosphorylation of S225 and T272 is required for the efficient nuclear import of myopodin.
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Discussion |
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Nuclear import is regulated at several levels. The binding of NLS-containing proteins to cytosolic receptors of the importin/karyopherin superfamily is critical because the affinity of this interaction determines transport efficiency (Weis, 2003). Therefore, targeting sequence recognition is a key control point in the regulation of nuclear import (Jans et al., 2000). One way to regulate nuclear transport is by changing the phosphorylation status of the cargo protein. Phosphorylation of the SV40 large tumor antigen and the Drosophila melanogaster morphogen dorsal upstream of the NLSs directly enhances the affinity between NLSs and importin /ß1 (Hubner et al., 1997; Briggs et al., 1998). The deletion of the phosphorylation sites in these nuclear cargoes causes a decreased nuclear import rate, which reveals the importance of serine/threonine phosphorylation for nuclear import efficiency. Phosphorylation of a cargo protein may modulate the affinity between NLS and importin
or may cause a conformational change in the cargo, thereby exposing an NLS (Harreman et al., 2004). In addition, phosphorylation may cause the release or binding of a heterologous, NLS-masking protein.
Recent proteomic analyses of 14-3-3binding proteins revealed that importins were putative 14-3-3 targets (Meek et al., 2004). However, 14-3-3 proteins do not have an intrinsic NLS, and, therefore, the direct binding of importins to 14-3-3 is unlikely (Brunet et al., 2002). Instead, these interactions are indirect and are mediated by proteins like myopodin that bind both 14-3-3 proteins and importin (Fig. 3 D).
14-3-3 proteins are highly acidic, chaperone-like molecules that can induce conformational changes in their binding partners. Myopodin, on the other hand, is a very basic protein with an isolectric point of 9.34 (Weins et al., 2001). Hence, it seems conceivable that 14-3-3 binding neutralizes the negative charge of myopodin. This, in turn, may change the structure of myopodin, thereby unmasking and exposing NLSs. In this study, we have shown that myopodin contains two functional 14-3-3binding sites that are required for efficient 14-3-3 binding (Fig. 1 H). Because each monomer of a 14-3-3 dimer binds its target in opposite directions (Yaffe et al., 1997), the binding of a 14-3-3 dimer could change the predicted linear structure of myopodin (Weins et al., 2001) to a U-shaped conformation. This is a potential regulatory conformational change in myopodin that could alter its biochemical features, such as the capability to interact with other proteins (e.g., importin ). Clearly, structural analysis of the myopodin14-3-3 interaction will be required to confirm or refute this hypothesis.
14-3-3 proteins can participate in protein translocation into mitochondria (Alam et al., 1994) and chloroplasts (May and Soll, 2000) by serving as cytoplasmic chaperones. In this study, we describe a novel role of 14-3-3 as a regulator of nuclear import by showing that 14-3-3 binding enables myopodin to interact with importin . Protein import into mitochondria and chloroplasts is mechanistically and evolutionary very different from nuclear import. Nevertheless, the results of this study suggest that cargoes with different subcellular destinations may share 14-3-3 as a cytoplasmic chaperone in order to achieve an import-compatible structure.
14-3-3 proteins are involved in the regulation of cell proliferation, differentiation, and cell death (van Hemert et al., 2001). For example, the epithelial 14-3-3 isoform serves as a tumor suppressor (Hermeking, 2003), and the loss of 14-3-3
expression can cause cell transformation (Dellambra et al., 2000). Several studies have shown a decrease or loss of 14-3-3
expression in transformed cells (Simooka et al., 2004; Urano et al., 2004), including transitional urinary bladder carcinomas (Ostergaard et al., 1997; Moreira et al., 2004). Myopodin not only binds to 14-3-3
but also acts as a tumor suppressor in bladder carcinomas (Sanchez-Carbayo et al., 2003). In fact, the relocalization of myopodin from the nucleus to the cytoplasm predicts the clinical outcome of these tumors (Sanchez-Carbayo et al., 2003). Based on the results of this study, it is tempting to speculate that the loss of nuclear myopodin in invasive bladder tumors results from the loss of 14-3-3
expression or from the loss of myopodin phosphorylation, which, in turn, abrogates 14-3-3 and importin
binding. Future studies will explore the phosphorylation stage of myopodin in normal urothelium and in bladder tumors.
To summarize, this study has helped to identify a functional role for 14-3-3 in promoting nuclear import. In particular, we have shown that importin binding and the subsequent nuclear import of the tumor suppressor myopodin are regulated by the serine/threonine phosphorylation-dependent binding of myopodin to 14-3-3 (Fig. 6). Altogether, these data provide a novel paradigm for the regulation of nuclear import by 14-3-3 in that 14-3-3 regulates the binding of a phosphorylated cargo protein to importin
and the nuclear import machinery.
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Materials and methods |
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Cloning and vectors
All cDNA fragments used in this study were amplified by RT-PCR using Pfu DNA polymerase (Stratagene) and were cloned into pEGFP-N1 (CLONTECH Laboratories, Inc.), pFLAG-cytomegalo virus-5a, b, and c (Sigma-Aldrich), or a modified glutathione S-transferase fusion vector (pGEX; Ron and Dressler, 1992), which was provided by Ben Margolis (University of Michigan Medical School, Ann Arbor, MI). Point mutations were generated with the QuikChange Multi Site-Directed Mutagenesis Kit (Stratagene). All constructs were verified by DNA sequencing. Mouse myopodin cDNA and its mutated variants were cloned into pEGFP-N1 and pFLAG vectors. GST14-3-3ß, , and
cDNAs were provided by Andrey Shaw (Washington University School of Medicine, St. Louis, MO), and GST14-3-3
K49E,
,
,
, and
were provided by Michael Yaffe (Massachusetts Institute of Technology, Cambridge, MA). The 14-3-3ß cDNA and the NH2-terminal deletion mutant ß
N (aa 128246) were subcloned into pEGFP-N1, pFLAG, and pGEX. The X. laevis importin
-1a cDNA (provided by Ian Mattaj, European Molecular Biology Laboratory, Heidelberg, Germany) was cloned into pGEX. The GFPC19 construct was provided by Ganjam Kalpana (Albert Einstein College of Medicine, Bronx, NY).
Antibodies
Rabbit antimyopodin (Weins et al., 2001) and rabbit antiX. laevis importin (Hachet et al., 2004) have been previously described. Rabbit anti14-3-3ß (C-20) was purchased from Santa Cruz Biotechnology, Inc.; the mouse monoclonal anti
-actinin antibody (sarcomeric, clone EA-53) was purchased from Sigma-Aldrich; and the goat anti-GST was purchased from Amersham Biosciences. HRP-coupled secondary antibodies were purchased from Promega. For immunofluorescence, Texas redconjugated goat antirabbit (Jackson ImmunoResearch Laboratories) and AlexaFluor488 (Molecular Probes) or Cy3-conjugated goat antimouse (Jackson ImmunoResearch Laboratories) were used. For immunoprecipitation of FLAG fusion proteins, monoclonal antiFLAG-M2 antibody that was covalently attached to agarose (Sigma-Aldrich) was used. GFP fusion proteins were detected with rabbit anti-GFP (Living Colors A.v. peptide antibody; CLONTECH Laboratories, Inc.), and FLAG fusion proteins were detected with antiFLAG-M2 antibody.
Cell culture and transfection
Cell culture of C2C12 myoblasts and HEK-293 cells as well as heat shock were performed as described previously (Weins et al., 2001). For biochemical analyses, transient transfections were performed using Lipofectamin2000 (Invitrogen).
Immunofluorescence and confocal microscopy
Immunofluorescence microscopy was performed as described previously (Weins et al., 2001). Anti14-3-3ß was used at 1:50; anti-actinin was used at 1:1,000; rhodamine-labeled phalloidin (Molecular Probes) was used at 1:500; and DAPI was used at 1:5,000.
Quantification of nuclear myopodin
We used a high resolution Nomarski objective (60x, NA 1.4; Olympus) and a high resolution epifluorescence with a precision stepper motor (model 99S001; Ludl) to measure the depth of individual cells. The adherent cells ranged from 5 to 8 µm in depth. The plasma membrane was stretched tightly over the top and bottom of the nuclei in these cells, and the nucleus appeared to span the entire depth of the cell. For the quantitative analysis of the localization of GFP fusion proteins, phase-contrast images of transfected myoblasts were collected. Using a widefield microscope system (model IX70; Olympus), the axial depth that was detected was deeper than 5 µm, and, therefore, fluorescence was accurately detected from the entire depth of the cells. To analyze transfected cells, whole cell areas, nuclei, and backgrounds of 25 cells per construct were traced using IPLab Image Analysis software (Scanalytics). For further calculations, Excel software (Microsoft) was used. The mean background level was subtracted from the means of the entire cell and the nucleus alone. To determine the content of GFP fusion protein in the whole cell versus that in the nucleus, the mean intensity of the entire cell versus the nucleus was multiplied by the area of each. Total GFP fusion protein in the cytoplasm was calculated by subtracting the mass of the nucleus from the mass of the total cell. The percentage of nuclear myopodin was calculated by dividing the mass of the nucleus by the mass of the cytoplasm.
Tissue and cell lysates
GST pull-down experiments were performed as described previously (Schwarz et al., 2001). For coimmunoprecipitation of endogenous proteins from the mouse heart, the tissue was homogenized in extraction buffer (50 mM Tris, pH 7.5, 150 mM NaCl, 0.1% SDS, 0.5% deoxycholic acid, and 1% NP-40) in a 1:3 wt/vol ratio. To purify FLAG-tagged proteins, HEK-293 cells were cultured in a 10-cm dish to 90% confluence and were transfected with FLAG cDNA constructs. After 24 h, cells were washed with PBS and were harvested with a cell scraper in 900 µl of modified radioimmunoprecipitation buffer (40 mM Tris, pH 7.4, 200 mM NaCl, 1% Triton X-100, 0.25% deoxycholic acid, 1 mM EDTA, and 1 mM EGTA). Lysates were incubated on ice for 30 min and were cleared at 14,000 g for 15 min at 4°C. For coimmunoprecipitation of FLAG- or GFP-tagged fusion proteins, HEK-293 cells were grown on a 10-cm dish, were cotransfected at a confluence of
90%, and were harvested on ice after 24 h using 5 ml PBS/50 mM EDTA. Cells were pelleted using centrifugation at 1,000 g for 5 min at 4°C and were washed twice with 5 ml PBS. For cell lysis, the pellet was resuspended in 1 ml of immunoprecipitation (IP) buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 50 mM KCl, 10 mM EDTA, 10 mM EGTA, 1.5% Triton X-100, and 0.75% NP-40) and was incubated on ice for 30 min. The cell lysate was cleared by centrifugation for 10 min at 5,000 g. To coimmunoprecipitate endogenous 14-3-3ß from myoblasts, C2C12 cells were grown on a 10-cm dish and were transfected with FLAG constructs at a confluence of
90%. FLAG fusion proteins were isolated as described for HEK-293 cells. For X. laevis protein extracts, 100 2-d-old embryos were homogenized in 2 ml IP buffer. After centrifuging at 10,000 g for 30 min at 4°C, the supernatant was used for binding studies.
Western blotting and endogenous coimmunoprecipitation
SDS-PAGE and Western blotting were performed as described previously (Mundel et al., 1997; Weins et al., 2001). Antibodies were used at the following ratios: myopodin-specific antibody SRIB2 at 1:300; anti14-3-3ß antibody at 1:2,000; antiimportin antibody at 1:1,000; antibody against FLAG at 1:10,000; antibody against GFP at 1:300; anti-GST at 1:10,000; and HRP-conjugated secondary antibodies at 1:20,000. The immunoreaction was visualized by ECL (Amersham Biosciences). To immunoprecipitate endogenous protein complexes, 1 ml of mouse heart extract was incubated overnight with 2 µg of antibodies at 4°C under rotation. Immune complexes were precipitated with 50 µl of protein A/GSepharose (Sigma-Aldrich) and were eluted in 100 µl SDS sample buffer. Anti-GFP IgG served as a negative control.
GST-binding assays
GST pull-down studies from tissue and cell extracts were performed as reported previously (Schwarz et al., 2001). To study the interaction between GST fusion proteins and FLAG-tagged proteins, GST proteins were expressed in bacteria and were purified using glutathione-coupled agarose as described previously (Schwarz et al., 2001). 1 µg of purified FLAG fusion protein in 500 µl PBS was added to the glutathione beads, and the reaction was incubated at 4°C under rotation for 2 h. For 14-3-3 inhibition studies, 1 mM R18 peptide (provided by Thomas McDonald, Albert Einstein College of Medicine; Wang et al., 1999) was added to the FLAG fusion protein in PBS. For the triple binding assay with purified importin , myopodin, and 14-3-3ß, the immobilized GST fusion protein was incubated with 1 µg each of two purified FLAG proteins. Silver staining and Western blot analysis confirmed the purity of FLAG-tagged myopodin and FLAG-tagged 14-3-3ß. The beads were collected by centrifugation and were washed five times in 1 ml PBS, and bound proteins were eluted by boiling in 150 µl Laemmli buffer. Eluates were analyzed by SDS-PAGE and by immunoblotting.
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Acknowledgments |
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Jun Oh was supported by the Deutsche Forschungsgemeinschaft and the Kidney and Urology Foundation of America. This work was supported by the National Institutes of Health grants DA18886, DK57683, and DK062472 to P. Mundel and grant AR41480 to R.H. Singer.
Submitted: 29 November 2004
Accepted: 10 March 2005
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References |
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---|
Alam, R., N. Hachiya, M. Sakaguchi, S. Kawabata, S. Iwanaga, M. Kitajima, K. Mihara, and T. Omura. 1994. cDNA cloning and characterization of mitochondrial import stimulation factor (MSF) purified from rat liver cytosol. J. Biochem. (Tokyo). 116:416425.[Abstract]
Bihn, E.A., A.L. Paul, S.W. Wang, G.W. Erdos, and R.J. Ferl. 1997. Localization of 14-3-3 proteins in the nuclei of arabidopsis and maize. Plant J. 12:14391445.[CrossRef][Medline]
Briggs, L.J., D. Stein, J. Goltz, V.C. Corrigan, A. Efthymiadis, S. Hubner, and D.A. Jans. 1998. The cAMP-dependent protein kinase site (Ser312) enhances dorsal nuclear import through facilitating nuclear localization sequence/importin interaction. J. Biol. Chem. 273:2274522752.
Brunet, A., F. Kanai, J. Stehn, J. Xu, D. Sarbassova, J.V. Frangioni, S.N. Dalal, J.A. DeCaprio, M.E. Greenberg, and M.B. Yaffe. 2002. 14-3-3 transits to the nucleus and participates in dynamic nucleocytoplasmic transport. J. Cell Biol. 156:817828.
Cahill, C.M., G. Tzivion, N. Nasrin, S. Ogg, J. Dore, G. Ruvkun, and M. Alexander-Bridges. 2001. Phosphatidylinositol 3-kinase signaling inhibits DAF-16 DNA binding and function via 14-3-3-dependent and 14-3-3-independent pathways. J. Biol. Chem. 276:1340213410.
Celis, J.E., B. Gesser, H.H. Rasmussen, P. Madsen, H. Leffers, K. Dejgaard, B. Honore, E. Olsen, G. Ratz, J.B. Lauridsen, et al. 1990. Comprehensive two-dimensional gel protein databases offer a global approach to the analysis of human cells: the transformed amnion cells (AMA) master database and its link to genome DNA sequence data. Electrophoresis. 11:9891071.[Medline]
Craig, E., Z.K. Zhang, K.P. Davies, and G.V. Kalpana. 2002. A masked NES in INI1/hSNF5 mediates hCRM1-dependent nuclear export: implications for tumorigenesis. EMBO J. 21:3142.
Dalal, S.N., C.M. Schweitzer, J. Gan, and J.A. DeCaprio. 1999. Cytoplasmic localization of human cdc25C during interphase requires an intact 14-3-3 binding site. Mol. Cell. Biol. 19:44654479.
Dellambra, E., O. Golisano, S. Bondanza, E. Siviero, P. Lacal, M. Molinari, S. D'Atri, and M. De Luca. 2000. Downregulation of 14-3-3 prevents clonal evolution and leads to immortalization of primary human keratinocytes. J. Cell Biol. 149:11171130.
Fu, H., R.R. Subramanian, and S.C. Masters. 2000. 14-3-3 proteins: structure, function, and regulation. Annu. Rev. Pharmacol. Toxicol. 40:617647.[CrossRef][Medline]
Grozinger, C.M., and S.L. Schreiber. 2000. Regulation of histone deacetylase 4 and 5 and transcriptional activity by 14-3-3-dependent cellular localization. Proc. Natl. Acad. Sci. USA. 97:78357840.
Hachet, V., T. Kocher, M. Wilm, and I.W. Mattaj. 2004. Importin alpha associates with membranes and participates in nuclear envelope assembly in vitro. EMBO J. 23:15261535.
Harreman, M.T., T.M. Kline, H.G. Milford, M.B. Harben, A.E. Hodel, and A.H. Corbett. 2004. Regulation of nuclear import by phosphorylation adjacent to nuclear localization signals. J. Biol. Chem. 279:2061320621.
Hermeking, H. 2003. The 14-3-3 cancer connection. Nat. Rev. Cancer. 3:931943.[CrossRef][Medline]
Hubner, S., C.Y. Xiao, and D.A. Jans. 1997. The protein kinase CK2 site (Ser111/112) enhances recognition of the simian virus 40 large T-antigen nuclear localization sequence by importin. J. Biol. Chem. 272:1719117195.
Jans, D.A., C.Y. Xiao, and M.H. Lam. 2000. Nuclear targeting signal recognition: a key control point in nuclear transport? Bioessays. 22:532544.[CrossRef][Medline]
Kalderon, D., B.L. Roberts, W.D. Richardson, and A.E. Smith. 1984. A short amino acid sequence able to specify nuclear location. Cell. 39:499509.[CrossRef][Medline]
Kumagai, A., and W.G. Dunphy. 1999. Binding of 14-3-3 proteins and nuclear export control the intracellular localization of the mitotic inducer Cdc25. Genes Dev. 13:10671072.
Lanford, R.E., and J.S. Butel. 1984. Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen. Cell. 37:801813.[CrossRef][Medline]
Liu, D., J. Bienkowska, C. Petosa, R.J. Collier, H. Fu, and R. Liddington. 1995. Crystal structure of the zeta isoform of the 14-3-3 protein. Nature. 376:191194.[CrossRef][Medline]
May, T., and J. Soll. 2000. 14-3-3 proteins form a guidance complex with chloroplast precursor proteins in plants. Plant Cell. 12:5364.
McKinsey, T.A., C.L. Zhang, and E.N. Olson. 2000. Activation of the myocyte enhancer factor-2 transcription factor by calcium/calmodulin-dependent protein kinase-stimulated binding of 14-3-3 to histone deacetylase 5. Proc. Natl. Acad. Sci. USA. 97:1440014405.
Meek, S.E., W.S. Lane, and H. Piwnica-Worms. 2004. Comprehensive proteomic analysis of interphase and mitotic 14-3-3-binding proteins. J. Biol. Chem. 279:3204632054.
Moreira, J.M., P. Gromov, and J.E. Celis. 2004. Expression of the tumor suppressor protein 14-3-3 sigma is down-regulated in invasive transitional cell carcinomas of the urinary bladder undergoing epithelial-to-mesenchymal transition. Mol. Cell. Proteomics. 3:410419.
Mundel, P., H.W. Heid, T.M. Mundel, M. Kruger, J. Reiser, and W. Kriz. 1997. Synaptopodin: an actin-associated protein in telencephalic dendrites and renal podocytes. J. Cell Biol. 139:193204.
Muslin, A.J., and H. Xing. 2000. 14-3-3 proteins: regulation of subcellular localization by molecular interference. Cell. Signal. 12:703709.[CrossRef][Medline]
Muslin, A.J., J.W. Tanner, P.M. Allen, and A.S. Shaw. 1996. Interaction of 14-3-3 with signaling proteins is mediated by the recognition of phosphoserine. Cell. 84:889897.[CrossRef][Medline]
Ostergaard, M., H.H. Rasmussen, H.V. Nielsen, H. Vorum, T.F. Orntoft, H. Wolf, and J.E. Celis. 1997. Proteome profiling of bladder squamous cell carcinomas: identification of markers that define their degree of differentiation. Cancer Res. 57:41114117.[Abstract]
Pan, S., P.C. Sehnke, R.J. Ferl, and W.B. Gurley. 1999. Specific interactions with TBP and TFIIB in vitro suggest that 14-3-3 proteins may participate in the regulation of transcription when part of a DNA binding complex. Plant Cell. 11:15911602.
Ron, D., and H. Dressler. 1992. pGSTaga versatile bacterial expression plasmid for enzymatic labeling of recombinant proteins. Biotechniques. 13:866869.[Medline]
Sanchez-Carbayo, M., K. Schwarz, E. Charytonowicz, C. Cordon-Cardo, and P. Mundel. 2003. Tumor suppressor role for myopodin in bladder cancer: loss of nuclear expression of myopodin is cell-cycle dependent and predicts clinical outcome. Oncogene. 22:52985305.[CrossRef][Medline]
Schwarz, K., M. Simons, J. Reiser, M.A. Saleem, C. Faul, W. Kriz, A.S. Shaw, L.B. Holzman, and P. Mundel. 2001. Podocin, a raft-associated component of the glomerular slit diaphragm, interacts with CD2AP and nephrin. J. Clin. Invest. 108:16211629.
Sehnke, P.C., R. Henry, K. Cline, and R.J. Ferl. 2000. Interaction of a plant 14-3-3 protein with the signal peptide of a thylakoid-targeted chloroplast precursor protein and the presence of 14-3-3 isoforms in the chloroplast stroma. Plant Physiol. 122:235242.
Seimiya, H., H. Sawada, Y. Muramatsu, M. Shimizu, K. Ohko, K. Yamane, and T. Tsuruo. 2000. Involvement of 14-3-3 proteins in nuclear localization of telomerase. EMBO J. 19:26522661.
Simooka, H., T. Oyama, T. Sano, J. Horiguchi, and T. Nakajima. 2004. Immunohistochemical analysis of 14-3-3 sigma and related proteins in hyperplastic and neoplastic breast lesions, with particular reference to early carcinogenesis. Pathol. Int. 54:595602.[CrossRef][Medline]
Todd, A., N. Cossons, A. Aitken, G.B. Price, and M. Zannis-Hadjopoulos. 1998. Human cruciform binding protein belongs to the 14-3-3 family. Biochemistry. 37:1431714325.[CrossRef][Medline]
Tzivion, G., Y.H. Shen, and J. Zhu. 2001. 14-3-3 proteins; bringing new definitions to scaffolding. Oncogene. 20:63316338.[CrossRef][Medline]
Urano, T., S. Takahashi, T. Suzuki, T. Fujimura, M. Fujita, J. Kumagai, K. Horie-Inoue, H. Sasano, T. Kitamura, Y. Ouchi, and S. Inoue. 2004. 14-3-3sigma is down-regulated in humar prostate cancer. Biochem. Biophys. Res. Commun. 319:795800.[CrossRef][Medline]
van Hemert, M.J., H.Y. Steensma, and G.P. van Heusden. 2001. 14-3-3 proteins: key regulators of cell division, signalling and apoptosis. Bioessays. 23:936946.[CrossRef][Medline]
Wang, B., H. Yang, Y.C. Liu, T. Jelinek, L. Zhang, E. Ruoslahti, and H. Fu. 1999. Isolation of high-affinity peptide antagonists of 14-3-3 proteins by phage display. Biochemistry. 38:1249912504.[CrossRef][Medline]
Wang, A.H., M.J. Kruhlak, J. Wu, N.R. Bertos, M. Vezmar, B.I. Posner, D.P. Bazett-Jones, and X.J. Yang. 2000. Regulation of histone deacetylase 4 by binding of 14-3-3 proteins. Mol. Cell. Biol. 20:69046912.
Weins, A., K. Schwarz, C. Faul, L. Barisoni, W.A. Linke, and P. Mundel. 2001. Differentiation- and stress-dependent nuclear cytoplasmic redistribution of myopodin, a novel actin-bundling protein. J. Cell Biol. 155:393404.
Weis, K. 1998. Importins and exportins: how to get in and out of the nucleus. Trends Biochem. Sci. 23:185189.[CrossRef][Medline]
Weis, K. 2003. Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle. Cell. 112:441451.[CrossRef][Medline]
Xiao, B., S.J. Smerdon, D.H. Jones, G.G. Dodson, Y. Soneji, A. Aitken, and S.J. Gamblin. 1995. Structure of a 14-3-3 protein and implications for coordination of multiple signalling pathways. Nature. 376:188191.[CrossRef][Medline]
Yaffe, M.B. 2002. How do 14-3-3 proteins work?Gatekeeper phosphorylation and the molecular anvil hypothesis. FEBS Lett. 513:5357.[CrossRef][Medline]
Yaffe, M.B., K. Rittinger, S. Volinia, P.R. Caron, A. Aitken, H. Leffers, S.J. Gamblin, S.J. Smerdon, and L.C. Cantley. 1997. The structural basis for 14-3-3: phosphopeptide binding specificity. Cell. 91:961971.[CrossRef][Medline]
Zhang, L., H. Wang, D. Liu, R. Liddington, and H. Fu. 1997. Raf-1 kinase and exoenzyme S interact with 14-3-3zeta through a common site involving lysine 49. J. Biol. Chem. 272:1371713724.
Zhang, S., H. Xing, and A.J. Muslin. 1999. Nuclear localization of protein kinase U-alpha is regulated by 14-3-3. J. Biol. Chem. 274:2486524872.
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