Article |
Address correspondence to James E. Rothman, Dept. of Cellular Biochemistry and Biophysics, Memorial Sloan-Kettering Cancer Center, 1275 York Ave., Box 251, New York, NY 10021. Tel.: (212) 639-8598. Fax: (212) 717-3604. E-mail: j-rothman{at}ski.mskcc.org
![]() |
Abstract |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Key Words: SNAP-25; liposome; syntaxin; vesicle; VAMP
![]() |
Introduction |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
The core of the SNARE complex is a bundle of four helices: three from the t-SNARE and one from the v-SNARE (Fasshauer et al., 1998; Poirier et al., 1998b; Sutton et al., 1998). Each helix consists of a conserved SNARE motif, a specialized heptad repeat domain (Weimbs et al., 1998). In neurons, the principal v-SNARE responsible for regulated exocytosis is vesicle-associated membrane protein 2 (VAMP2);* the principal t-SNARE is a heterodimer of syntaxin 1A and the peripheral membrane protein, synaptosomal-associated protein of 25 kD (SNAP-25; Söllner et al., 1993). SNAP-25 contains two SNARE motifs; syntaxin 1A and VAMP2 each contain one such domain.
Deeper understanding of the fusion reaction and the basis for its regulation will require further insights into the choreographed pathway by which v-SNAREs and t-SNAREs assemble. At least two steps of the SNARE assembly pathway are under the control of syntaxin's NH2-terminal regulatory domain (NRD) which is itself composed of two parts: a separately folding, three-helix bundle termed Habc (Fernandez et al., 1998), and an 40-residue linker domain that connects Habc to the coil domain of syntaxin 1A. The NRD of isolated syntaxin folds back onto the SNARE motif (Dulubova et al., 1999), and this closed conformation is stabilized by the binding of members of the Sec1 family (Misura et al., 2000). Mutations in the linker region of the NRD destabilize the closed conformation (Dulubova et al., 1999; Misura et al., 2000). Studies on the homologous plasma membrane yeast t-SNARE subunits have demonstrated that the closed conformation of the syntaxin (Sso1) is a kinetic deterrent to t-SNARE complex formation with Sec9 (Munson et al., 2000). Likewise, in the known crystal structure of the n-Sec1Syntaxin complex, critical SNAP-25 binding sites are occluded (Misura et al., 2000), suggesting that the NRD may also play a role in this system in regulating t-SNARE assembly that we call the "NRD assembly switch."
In addition to regulating t-SNARE assembly, the NRD also controls SNAREpin (v-/t-SNARE trans-complex) formation and thereby, the rate of the fusion reaction. Because this step links up two membranes, we refer to it as the "NRD docking switch." Like the NRD assembly switch, the docking switch is constitutively "off," and as a result, fusion mediated by isolated v- and t-SNAREs is intrinsically very slow. However, when the NRD is removed, the docking switch is now constitutively "on," so fusion is now greatly accelerated and its rate is now limited by steps after docking (Parlati et al., 1999).
Because the fusion reaction is driven by the assembly of unfolded or partially unfolded helical domains in the individual SNARE proteins, it will be critical to relate structurally defined folding intermediates to functionally defined stages in the fusion process. Structural arguments have suggested that the helical bundle that comprises the SNAREpin "zips" progressively toward the bilayers as its heptad-repeat v- and t-SNARE cytoplasmic domains fold up, beginning at their membrane-distal NH2-termini and progressing to their membrane-proximal COOH termini (Fasshauer et al., 1998; Fiebig et al., 1999; Lin and Scheller, 2000). Currently, the most direct evidence for this mechanism comes from the selective effects of conformationally specific proteolytic neurotoxins and antibodies on the kinetics and physiology of exocytosis in situ (Hua and Charlton, 1999; Xu et al., 1999). These results strongly imply that the SNAREpins are partly (but not completely) zipped-up only one or a few milliseconds before the opening of the fusion pore, and in doing so, provide a powerful confirmation of the direct role SNARE assembly plays in bilayer merger in vivo, ruling out alternative mechanisms.
Here, we provide the first functional test of the zippering model using structurally targeted peptides as inhibitors of SNARE assembly. If fusion requires zippering in the NC direction, then a reversibly dissociating peptide that binds to the NH2-terminal portion of the t-SNARE will block the first contact between v- and t-SNAREs, and thus inhibit fusion initially. However, once zippering has already begun, fusion will be resistant to the NH2-terminally directed peptide. By contrast, a peptide that binds reversibly to the COOH-terminal portion of the t-SNARE will still allow zippering to begin and will allow fusion to proceed at a rate determined by how fast it is displaced as the SNAREs zip-up in what is now an intramolecular process. Other models have distinct outcomes. For example, if zippering were in the opposite C
N direction, then the NH2- and COOH-peptide would have the opposite properties.
Our results further support that SNAREpin assembly between bilayers is polarized, and that zippering must occur in the NC direction for fusion to result. But unexpectedly, the COOH-peptide dramatically increases the rate of fusion, revealing an intrinsic regulatory switch on the t-SNARE that controls the rate of zippering that we term the "tC fusion switch." When the tC fusion switch is off, zippering of the SNAREpin is blocked approximately midway, potentially corresponding to the state of SNAREpins in vesicles that are available for ready release after a signal for exocytosis (Hua and Charlton, 1999; Xu et al., 1999). When the tC fusion switch is turned on, zippering can be rapidly completed and fusion results. Furthermore, while we were in the process of characterizing this tC fusion switch for the mammalian exocytic SNAREs, we established that a similar COOH-peptidedependent increase in fusion could be observed with yeast endosomal or Golgi-related SNARE complexes (Paumet et al., 2001; Parlati et al., 2002), demonstrating the conservation of this regulatory switch. We suggest how regulatory proteins could control the tC fusion switch and therefore the rate of membrane fusion.
![]() |
Results |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
To explore the hypothesis of polarized zippering, which predicts that the membrane-distal and membrane-proximal regions of the SNAREpins are functionally asymmetric, we initially chose peptides corresponding to the NH2-terminal and COOH-terminal halves of the SNARE motif of the exocytic v-SNARE VAMP2, termed vN-pep and vC-pep, respectively (Fig. 1). Together, the sequences of vN-pep and vC-pep comprise the complete sequence of the coil region of VAMP2. The dividing line between them is after arginine 56, chosen to correspond to the hydrophilic "zero layer" in the otherwise hydrophobic core of the coiled coil (Sutton et al., 1998).
|
|
Donor liposomes bearing vC-pep-cys-C45 do not themselves fuse with t-liposomes, but they fuse efficiently when free vN-pep is added (Fig. 2 B). Fusion is vN-pep dose-dependent, and is inhibited by either the complete cytoplasmic domain of VAMP2 (v-cyt) or free vC-pep (unpublished data). Free vC-pep inhibits rather than activates, because free vC-pep + vN-pep results in irreversible occupation of the VAMP2 binding site on the t-SNARE as observed in Fig. 1 A. Evidently, continuity through the length of the v-SNARE coil is not an essential feature for fusion. Fusion does not occur when vN-pep (whether joined to C45 maleimide via a COOH- or NH-terminal Cys-SH) is anchored to the donor liposomes and free vC-pep is added (unpublished data).
Fusion when one coil is separated into two pieces, forming a discontinuous helix, is not unprecedented, but is surprising for VAMP in light of some published results. For example, BoNT/E toxin treatment of cracked PC12 cells specifically removes the last 26 amino acids from SNAP-25, rendering it incapable of forming an SDS-resistant complex (Hayashi et al., 1994) and incapable of participating in neuroendocrine exocytosis (Chen et al., 1999). However, exocytosis can be recovered if a peptide correlating to the cleaved fragment is added back at high concentration (Chen et al., 2001). Cleavage of VAMP2 by BoNT/D releases the NH2-terminal 59 amino acids from the protein and eliminates exocytosis. However, in this case, exocytosis cannot be recovered by addition of the cleaved fragment (Chen et al., 2001). Peptides that exactly correspond to the BoNT/D cleavage site (VAMP2 aa 2559 and 6094-cys) were equally efficient at mediating liposome fusion (unpublished data). The difference in the two studies perhaps indicates that the cleaved fragment is not stable in permeabilized cells.
The core SNARE complex migrates as an SDS-resistant band on SDSpolyacrylamide gels (Fasshauer et al., 1998; Poirier et al., 1998a; Fig. 2 C, lane 2; 65-kD band). This complex consists of the cytoplasmic domain of VAMP2 (v-cyt) bound to the cytoplasmic domain of the exocytic t-SNARE (t-cyt), a complex of the cytoplasmic domains of syntaxin and SNAP-25. No such SDS-resistant complex is formed between t-cyt and one or both peptides (Fig. 2 C, lanes 35). Preassembled v-cyt/t-cyt SDS-resistant complexes are unaffected by the addition of one or even both peptides (Fig. 2 C, lane 6). Also, when the t-SNARE is incubated with either vN-pep or vC-pep alone and then v-cyt is added, SDS-resistant complex formation is unaffected (Fig. 2 C, lanes 8 and 9). However, when the t-SNARE is preincubated with both vN-pep and vC-pep before the addition of v-cyt, SDS-resistant complex formation is now largely prevented (Fig. 2 C, lane 7). This establishes that vN-pep and vC-pep can bind simultaneously and stably to the t-SNARE, and when they are both bound, neither can be competed off by the v-SNARE.
Two conformations in the coiled-coil core of the t-SNARE detected by limited proteolysis
Two structural states of the coiled-coil core region of exocytic t-SNAREs have previously been characterized using proteins from different organisms. In the yeast Sso1Sec9 complex, the SNARE motif within the cytoplasmic domain of the t-SNARE is a three-stranded coiled coil in its approximately NH2-terminal half, but is less ordered in its approximately COOH-terminal half (Fiebig et al., 1999). The only structures available for the mammalian complex are limited to the coil-forming SNARE domains of syntaxin 1A and SNAP-25, which give rise to a four-helix bundle composed of the two coils from SNAP-25 and two syntaxin H3 coils (Margittai et al., 2001; Xiao et al., 2001). However, it is likely that the mammalian t-SNARE also goes through a three-stranded intermediate to allow for VAMP2 binding, and that such an intermediate would be structurally analogous to the yeast complex. In fact, EPR studies on the SNARE motifs of the mammalian t-SNARE complexed with an NH2-terminal fragment of the VAMP2 SNARE domain display significant disorder over the COOH-terminal half of the t-SNARE (Margittai et al., 2001). Furthermore, work in our lab suggests that the NRD, which is not present in the studies cited above, may be essential to maintain a 1:1 syntaxin/SNAP-25 stoichiometry (unpublished observations). When the core domains of the mammalian t-SNARE are bound to the cytoplasmic domain of the v-SNARE VAMP2, the t-SNARE is fully structured throughout as three strands of the resulting four-helix bundle (Sutton et al., 1998). So, although the partly bundled conformation in the isolated t-SNARE was initially identified by nuclear magnetic resonance and only with the yeast homologues, we will illustrate that a similar change from partly bundled to more completely structured can also conveniently be monitored in the mammalian t-SNARE using a limited proteolysis assay that is better suited for routine biochemical studies.
The SNARE complex yields a protease-resistant core limited to the four-SNARE motif coil regions (Fasshauer et al., 1998; Poirier et al., 1998a). Proteolysis of t-cyt alone yields a 14-kD band that originates from the Habc portion of the NRD (Fasshauer et al., 1998), and a distinct set of smaller (6 kD) peptide fragments (Fig. 3 A, lane 2), whose origin was established by a combination of Western blot analysis and NH2-terminal sequencing.
|
vC-pep structures the membrane-proximal portion of the t-SNARE
To monitor the effects of peptide addition on each SNAP-25 coil, we used antibodies directed against the extreme NH2 terminus or COOH terminus of SNAP-25. The addition of vC-pep (Fig. 3, B and C) shifts the proteolytic sensitivity of each coil in a manner identical to that observed with the complete v-cyt; SNAP-25N becomes shorter due to increased proteolysis of the adjoining loop (Fig. 3 B), and SNAP-25C becomes protected at its COOH terminus (the site of the antibody; Fig. 3 C), which is apparent in the Coomassie gel as an increase in the molecular mass of the band (Fig. 3 A). This implies that binding of vC-pep by itself shifts the conformational equilibrium of the COOH-terminal half of the t-SNARE to a more structured state: a tC fusion switch. vN-pep does not significantly change the protease digestion pattern of t-cyt.
We also performed the same analysis with the full-length t-SNARE inserted into lipid bilayer vesicles (Fig. 3 D). As a result of membrane insertion, now both H3 of syntaxin and SNAP-25N are protected from proteolysis to a much greater degree. No change in the pattern of the protection of SNAP-25N or H3 is now caused by adding either peptide or even v-cyt (unpublished data). The greater resistance to proteolytic cleavage of H3 of syntaxin and SNAP-25N when they are attached to the membrane has any of several explanations. Access to the t-SNARE could be sterically restricted by the close proximity of the lipid bilayer. Alternatively, this portion of the t-SNARE could be occluded within oligomers organizing a fusion "ring" on the bilayer surface. However, just as the specificity of SNARESNARE interactions is greatly influenced by membrane association (McNew et al., 2000a; Scales et al., 2000), it may well be that the range of conformations that the SNAREs can sample is altered in the context of membrane insertion. Interestingly, a dramatic increase in the protection of the COOH terminus of SNAP-25C is still observed when vC-pep is added (Fig. 3 D). Taken together, these results suggest that membrane-proximal portions of SNAP-25N and syntaxin H3 t-SNAREs have a more ordered structure when associated with a membrane than they do as a soluble complex. However, the COOH terminus of the COOH-terminal helix of SNAP-25 remains unstructured and proteolytically accessible in both the soluble and the bilayer-anchored t-SNAREs, and vC-pep binding thus protects this region of the t-SNARE.
C zippering during fusion">Polar effects of N versus C v-SNARE peptides imply NC zippering during fusion
Fig. 4 A shows that vN-pep reduces the initial rate of fusion between t- and v-liposomes to nearly zero. Inhibition is dose dependent and complete at close to 1 mol vN-pep/mol t-SNARE (Fig. 4 C). However, fusion eventually begins after several minutes, even when it had initially been completely inhibited by a saturating concentration (5.4 µM) of vN-pep (Fig. 4 B). This is expected because the higher affinity, irreversibly bound, full-length v-SNARE will eventually displace reversibly bound vN-pep at a rate determined by the rate of dissociation of vN-pep from the t-SNARE (Fig. 2 C). This is consistent with the NC zippering model and inconsistent with random (nonpolarized) zippering models.
|
|
At saturating concentrations of vC-pep, the initial rate of fusion corresponds to a half-time for one round of 4 min (t1/2 = 4.1 ± 1.5 min). This rate is faster than that observed when the syntaxin NRD is removed (t1/2 = 10 min; Parlati et al., 1999; Fig. 7 A) or when donor and acceptor liposomes are allowed to dock at 04°C before permitting fusion at 37°C (t1/2 = 7 min; Parlati et al., 1999). The t1/2 of fusion in the presence of vC-pep of 4.1 ± 1.5 min should be considered an upper limit because it must include the unknown time required for vC-pep to dissociate.
|
|
Rapid, reversible assembly of SNAREpins with closed t-SNAREs
The initial rate of fusion is inhibited by vN-pep binding to t-SNAREs, which blocks the initiation of zippering (Fig. 4 C). This implies that a binding site for the NH2-terminal portion of the v-SNARE is open and available for binding and necessary to initiate zippering. If this is correct, then it follows that if the v-SNARE were allowed to bind to initiate its zippering with the t-SNARE in the absence of vN-pep, then subsequently, fusion should be resistant to vN-pep.
In fact, within the first 2 min of incubation, the initial rate of fusion (Fig. 7) becomes largely resistant to the inhibitory effects of vN-pep. Resistance to vN-pep requires coincubation of v- with t-liposomes (not depicted). Yet, at these same early time points the liposomes have progressed through <20% of the first round of fusion, and therefore are unlikely to have completed zippering. This rapid acquisition of resistance to vN-pep implies that the v-SNARE initiates NC zippering with the t-SNARE, and that fusion is rate-limited by the slow completion of zippering at the membrane-proximal COOH termini of the SNAREs.
COOH-terminal deletions of SNAP-25 reduce SNARE-mediated fusion and abolish the stimulatory effect of vC-pep
The proteolysis results (Fig. 3) combined with the predicted binding site of vC-pep strongly suggest that the tC fusion switch is located near the COOH-terminal, membrane-proximal portion of the coiled-coil region on the t-SNARE, and specifically involves the COOH terminus of SNAP-25. To test this hypothesis, we deleted the COOH-terminal 9 and 26 amino acids of SNAP-25 to generate proteins that correspond to the well-characterized products of proteolysis by the botulinum neurotoxins A and E, respectively. Deletion of the nine COOH-terminal residues of SNAP-25 reduces the initial rate and final extent of liposome fusion (Fig. 8 A). Importantly, fusion by the SNAP-259 t-SNARE was still accelerated by removal of the NRD (from 0.5 to 1.0 rounds of fusion at 2 h; Fig. 8 B), indicating that the SNAP-25 deletion does not grossly change the structure of the t-SNARE, and confirming that the effects of the NRD switch are restricted to the NH2-terminal portion of the coiled coil. The BoNT/E-like SNAP-25
26 t-SNARE did not show any fusion under any conditions, including after removal of the NRD, despite the fact that the concentration of the t-SNAREs and the extent of proteolysis were comparable for all liposome preparations (Fig. 8 C). Addition of vC-pep had no effect on the fusogenic capacity of the SNAP-25
9 t-SNARE, even when present at a 10-fold excess and at concentrations in excess of 100 µM (Fig. 8 D). Therefore, in this t-SNARE that retains significant fusion activity and full control of the NRD switch, all regulation of the tC fusion switch has been lost, at least via the mechanism used by vC-pep.
|
|
![]() |
Discussion |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Examples of conformational switches controlling these steps are now coming to light. Post-fusion cis-SNARE complexes are disassembled and their subunits are recycled by the ATPase NSF and SNAP proteins (Söllner et al., 1993) using an ATP/ADP conformational switch that provides mechanical force to rip open the SNARE complex (Vale, 2000; Dalal and Hanson, 2001). Fusion can be regulated before SNAREpin assembly by switching the assembly of t-SNAREs from their subunits on and off. When the NRD assembly switch is off, the assembly of Sso1 and Sec9 to form the yeast exocytic t-SNARE is auto-inhibited by the NRD of the syntaxin heavy chain (Munson et al., 2000).
The rate of SNAREpin formation between v-SNARES and already assembled t-SNAREs on separate membranes is regulated through the distinct and later-acting NRD docking switch in the assembled t-SNARE. In the absence of other regulatory factors, this switch is off and fusion is slow. Removal of the NRD leaves the t-SNARE in a constitutive "on" state, and fusion is correspondingly accelerated (Parlati et al., 1999). The degree of structural similarity between the NRD assembly switch in isolated syntaxin and the NRD docking switch in the assembled syntaxinSNAP-25 exocytic t-SNARE is presently unknown, because the structure of the syntaxinSNAP-25 heterodimer has not been established. However, there must be some differences between the way the NRD interacts with the coil portion of the t-SNARE in the off state of the NRD assembly and docking switches because the linker portion of NRD binds the coil in an isolated yeast syntaxin at the same site at which a portion of SNAP-25 binds in the mammalian t-SNARE (Munson et al., 2000). Other proteins such as munc-13 may "open" the NRD assembly and/or docking switches in syntaxin by binding the NRD (Betz et al., 1997; Richmond et al., 2001).
The molecular mechanisms by which fusion is regulated during SNAREpin assembly are less clear. Certainly, it is clear that regulation of zippering is physiologically important (Hua and Charlton, 1999; Xu et al., 1999), and proteins such as synaptotagmin and complexin may act at this stage (Fernandez-Chacon et al., 2001; Reim et al., 2001; Tokumaru et al., 2001). In fact, recent structural studies suggest that complexin functions to stabilize contacts in the COOH-terminal half of the heterotrimeric core complex (Chen et al., 2002). The results presented in this paper argue that the SNARE complex obligatorily zippers in the NC direction (toward the membranes) as an intrinsic property of its coil regions. And, as importantly, there is an inherent molecular switch (the tC fusion switch) built into the zippering mechanism that controls the rate of zippering, and thus, fusion.
Recent structural studies have suggested that the SNARE motif of the exocytic t-SNARE is separated into distinct tN and tC domains, most likely by the highly polar zero layer located about halfway along its length (Hua and Charlton, 1999; Xu et al., 1999). The membrane-distal tN domain forms a stable three-stranded helical bundle, but in the membrane-proximal tC domain this bundle is inherently unstable (Fiebig et al., 1999). The data presented here establish that the tN and tC domains are functionally distinct with respect to bilayer fusion, and that the v-SNARE is similarly divided into corresponding cognate vN (membrane-distal) and vC (membrane-proximal) domains. Like their cognate tN and tC domains, the vN and vC domains can independently switch into and out of the helical bundle conformation, and independently pair with tN and tC. Polarized zippering results because vN pairs with tN before vC pairs with tC. The isolated vN and vC domains of the v-SNARE (vN-pep and vC-pep) can even drive bilayer fusion when vC-pep is lipid-anchored.
The simplest structural interpretation of our functional data is that in the NRD-docking off state, the NRD covers a portion of the coil domain, sterically inhibiting further zippering, but leaves at least part of the tN domain uncovered. vC-pep likely sterically competes with NRD binding to a portion of tC. vC-pep binding would then release NRD and throw this switch for docking. Simultaneously, vC-pep binding will also stabilize tC as a helical bundle, turning on this switch for fusion. We have already used the information gleaned here to design peptide activators of two other SNARE-dependent fusion pathways (Paumet et al., 2001; Parlati et al., 2002), which suggests that the structural changes and the opportunity for regulation are not limited to the neuronal secretory pathway.
Why is vC-pep more efficient at carrying out NRD displacement and tC structuring than the COOH-terminal part of the full-length v-SNARE? After the initial interaction between the v- and t-SNARE liposomes has occurred, which takes much less than 5 min (Fig. 7), one could suppose that the COOH terminus of VAMP2 is now present in a very high local concentration and would then be the more efficient activator. However, this is precisely the point at which fusion becomes energetically challenging, when two membranes are brought into close apposition, and water must be displaced before the bilayers will get any closer. The steric penalties for allowing the COOH termini of the v- and t-SNAREs to zip up become enormous. The free peptide does not invoke these penalties and therefore could bind (or remain bound) and activate the t-SNARE tC fusion switch more efficiently.
Once the t-SNARE is activated, zippering is an intrinsically more efficient process. Of course once the peptide dissociates, the already NH2-terminally associated VAMP2 would have a significant advantage in the completion of zippering due to its proximity and much higher affinity, provided that the t-SNARE remains activated for some finite period of time. It is perhaps a bit surprising that the peptide, which binds so tightly and overcomes two independent regulatory mechanisms, would then freely dissociate and not instead act as an impediment to the completion of fusion. Consistent with this view, we suspect that the rate of peptide dissociation (t1/2 = 35 min, as measured by protease sensitivity; unpublished data) is the limiting factor in our accelerated fusion, which has a half-time to one round of fusion on the order of 4 min.
One can easily imagine how cellular regulatory proteins could stabilize the helical bundle conformation of tC (analogous to the synthetic peptide, vC-pep), and in so doing, trigger fusion. Like vC-pep, they could do so at any time physiology demands (Fig. 5 C). It can also be imagined that cellular regulatory proteins might stabilize a noncoil conformation of tC, or permanently occupy or cover the v-SNARE binding site of tC. These proteins would block fusion, whereas reversal of their binding activity would activate fusion.
In summary, exocytic t-SNAREs possess distinct autoinhibitory docking and fusion switches that cellular regulatory proteins could control sequentially or independently (Fig. 9). When both the NRD docking switch and the tC fusion switch are off, fusion is extremely slow. When the NRD switch is thrown on, vesicles rapidly associate as vN fully zips up with tN. However, fusion is still slow because the SNAREpins cannot complete their zippering until tC is switched to its bundle, i.e., "on" conformation. When this happens, vC can then bind tC, zippering can be completed, and fusion can occur. Even though vC-pep binding turns both switches on, it is clear that the NRD and tC fusion switches are not obligatorily coupled. Thus, when NRD is physically removed, tC can still be switched on by vC-pep (Fig. 6), and when the SNAP-25 COOH terminus is distorted to inactivate the tC fusion switch, the NRD switch remains rate-limiting (Fig. 8). In fact, the ability to adopt a closed conformation need not be a universal feature of all syntaxins or t-SNAREs (Dulubova et al., 2001). Yet, all known syntaxins have a domain analogous to the NRD, and all known syntaxins and t-SNARE light chains have a central polar residue in the coil region that would divide other secretory t-SNAREs into potentially separately folding tN and tC domains, suggesting that one or both switches are likely present in most syntaxins. Furthermore, the steady-state accumulation of partially zipped SNAREpins in the docked, readily releasable pool of exocytic vesicles (Hua and Charlton, 1999; Xu et al., 1999) implies the two switches are in fact uncoupled physiologically.
![]() |
Materials and methods |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Lipid mixtures
Lipid mixtures were made at the indicated concentrations and then stored in sealed ampules at -80°C. The donor lipid mix included the following: 82.5 mol% 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphatidylcholine (POPC), 15 mol% 1,2-dioleolyl-sn-glycero-3-phosphatidylserine (DOPS), 1.5 mol% lissamine rhodamine B (rhodamine) 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine, 1.5 mol% NBD 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine, and 3 mM total lipid. The acceptor lipid mix included the following: 85 mol% POPC, 15 mol% DOPS, and 15 mM total lipid.
Plasmids
his6-SNAP-259 (a COOH-terminal deletion analogous to the product of botulinum-neurotoxin A proteolysis; amino acids 1197) was PCR-amplified from SNAP25b (pFP247) using oligonucleotides OFP 82(5'-TCCATGGCCCATCATCATCATCATCATGCCGAGGACGCAGACATGC-3') and oBJ4 (5'-GAATTCCTCGAGATTATTTATTTATTGGTTGGCTTCATCAATTCT-3'). This PCR product was digested with XhoI and NcoI restriction enzymes and inserted into plasmid pFP247 to create plasmid pBJ8.
his6-SNAP-2526 (a COOH-terminal deletion analogous to the product of botulinum-neurotoxin E proteolysis; amino acids 1180) was PCR-amplified from SNAP25b (pFP247) using oligonucleotides OFP82 and OBJ3 (5'-GAATTCCTGAGATTATTTATTTACCTGTCGATCTGGCG-3'). This PCR product was digested with XhoI and NcoI and inserted into plasmid pFP247 to create plasmid pBJ7.
Protein expression and purification
Full-length t-SNARE complex containing mouse his6-SNAP-25 and rat syntaxin 1A was expressed and purified from the polycistronic vector pTW34 (Weber et al., 2000). t-SNARE complex containing syntaxin, with a thrombin cleavage site at residue 181 and his6-SNAP-25, was expressed and purified from the polycistronic vector, pTW69 (Parlati et al., 1999). t-SNARE containing thrombin-cleavable syntaxin and either SNAP-259 or SNAP-25
26 was produced by the coexpression of pFP226 (Parlati et al., 1999) and either pBJ8 or pBJ7, respectively. The cytosolic domain of the t-SNARE complex with no internal cysteines (t-cyt) containing mouse his6-SNAP-25 (C
S) and rat syntaxin 1A (residues 1265-L-C, C145S) was expressed from BL21 (DE3) Escherichia coli cotransfected with pJM72 and pJM572 as described previously (McNew et al., 2000b). Full-length mouse VAMP2-his6 was expressed and purified from pTW2 as described previously (Weber et al., 1998). The cytosolic domain of mouse VAMP2 (v-cyt) with a COOH-terminal his6 tag was expressed from pET-rVAMP2CD and purified as described previously (Weber et al., 1998). In some experiments, v-cyt with a COOH-terminal cysteine and an NH2-terminal, his6 tag (McNew et al., 2000b), was also used as an inhibitor and gave identical results.
Protein reconstitution into liposomes
VAMP2 and t-SNARE complexes were reconstituted into liposomes by detergent dialysis and isolated on a Nycodenz flotation gradient as described previously (Weber et al., 1998). In all experiments, VAMP2 was reconstituted with the donor lipid mix, and t-SNARE was reconstituted with the acceptor lipid mix. To remove the NRD from thrombin-cleavable syntaxin, thrombin-cleavable t-SNARE liposomes were incubated with 0.02 U/µl thrombin (Sigma-Aldrich) for 1 h at RT. The proteolysis was stopped by the addition of 2 mM AEBSF, and the liposomes were immediately used in fusion experiments.
Proteinase K protection assay
Limited proteolysis by proteinase K (Boehringer) was used to identify protected components of the core assembly, or of t-cyt in the presence of VAMP peptides, as described with some modifications (Fasshauer et al., 1998). In brief, t-cyt or t-liposomes were incubated with proteinase K at a 40:1 protein/proteinase molar ratio for 10 min at RT. Proteolysis was stopped by the addition of 5 mM PMSF and 5 mM AEBSF, and the samples were immediately boiled for 5 min. Novex LDS sample buffer was added and the samples were boiled again. To test for VAMP or VAMP peptidedependent protection, v-cyt or VAMP peptides were added to t-cyt or to t-liposomes and preincubated for 20 min at RT before addition of proteinase K.
Electrophoresis and Western blotting
Electrophoresis was performed in precast 10 or 12% bis-Tris gels (Novex) running in MES buffer. For Western blot analysis, the protein fragments were transferred onto nitrocellulose and incubated with primary antibody for 30 min. Primary antibodies were used at the following dilutions: for HPC-1, 1:1,500 (syntaxin monoclonal), for 939-3, 1:1,000 (rabbit antibody raised against COOH-terminal peptide of SNAP-25; residues 207218), for Cl 71.1, 1:5,000 (SNAP-25 NH2-terminal monoclonal [Cat. No. 111001; Synaptic Systems]). Goat antimouse and goat antirabbit secondary antibodies conjugated to HRP (Bio-Rad Laboratories) were incubated with the blot for 20 min at a 1:2,000 dilution, and the blots were developed with the ECL Western blotting detection system.
Fusion assay
Fusion between t-SNARE liposomes and v-SNARE liposomes was monitored by the increase in fluorescence resulting from the dilution of a quenched FRET pair of fluorophores in the v-SNARE liposomes as described previously (Weber et al., 1998). For each assay, 45 µl of t-SNARE liposomes were mixed in 96-well FluoronuncTM polysorb plates (Nunc) at 37°C with reconstitution buffer or the indicated amounts of v-cyt or peptide for 210 min. Stock concentrations of peptide were adjusted so that 210 µl of peptide were added to each 50-µl reaction and the balance made up with buffer. The fusion reaction was initiated by adding 5 µl of RT v-liposomes. NBD fluorescence was monitored at 2-min intervals for 160 min (excitation = 460 nm; emission = 538 nm) in a Fluoroskan II plate reader (Thermo Labsystems) at 37°C. At 120 min, 10 µl of 2.5% wt/vol n-dodecyl-maltoside (Boehringer) was added to completely dissolve the lipids and measure the NBD fluorescence at infinite dilution. The data were normalized and calibrated as described previously in order to derive the rounds of fusion (Weber et al., 1998; Parlati et al., 1999).
![]() |
Footnotes |
---|
James A. McNew's present address is Dept. of Biochemistry and Cell Biology, Rice University, MS140, 6100 Main St., P.O. Box 1892, Houston, TX 77251.
Robert J. Johnston's present address is Dept. of Biochemistry and Molecular Biophysics, Columbia University, College of Physicians and Surgeons, 701 West 168th St., HHSC 724, Box 140, New York, NY 10032.
Frank Parlati's present address is Rigel Pharmaceuticals, Inc., 240 East Grand Ave., South San Francisco, CA 94080.
* Abbreviations used in this paper: NRD, NH2-terminal regulatory domain; SNAP-25, synaptosomal-associated protein of 25 kD; t-cyt, cytoplasmic domain of syntaxinSNAP-25 complex; VAMP, vesicle-associated membrane protein; v-cyt, cytoplasmic domain of VAMP.
![]() |
Acknowledgments |
---|
Research was supported by a National Institutes of Health (NIH) grant R01 DK 27044 (to J.E. Rothman) and an NIH postdoctoral fellowship (to J.A. McNew).
Submitted: 17 December 2001
Revised: 3 July 2002
Accepted: 8 July 2002
![]() |
References |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Betz, A., M. Okamoto, F. Benseler, and N. Brose. 1997. Direct interaction of the rat unc-13 homologue Munc 13-1 with the N-terminus of syntaxin. J. Biol. Chem. 272:25202526.
Block, M.R., B.S. Glick, C.A. Wilcox, F.T. Wieland, and J.E. Rothman. 1988. Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport. Proc. Natl. Acad. Sci. USA. 85:78527856.[Abstract]
Chen, X., D.R. Tomchick, E. Kovrigin, D. Arac, M. Machius, T.C. Sudhof, and J. Rizo. 2002. Three-dimensional structure of the complexin/SNARE complex. Neuron. 33:397409.[Medline]
Chen, Y.A., S.J. Scales, J.R. Jagath, and R.H. Scheller. 2001. A discontinuous SNAP-25 C-terminal coil supports exocytosis. J. Biol. Chem. 276:2850328508.
Dulubova, I., S. Sugita, S. Hill, M. Hosaka, I. Fernandez, T.C. Südhof, and J. Rizo. 1999. A conformational switch in syntaxin during exocytosis: role of munc18. EMBO J. 18:43724382.
Fasshauer, D., W.K. Eliason, A.T. Brünger, and R. Jahn. 1998. Identification of a minimal core of the synaptic SNARE complex sufficient for reversible assembly and disassembly. Biochemistry. 37:1035410362.[CrossRef][Medline]
Fernandez-Chacon, R., A. Konigstorfer, S.H. Gerber, J. Garcia, M.F. Matos, C.F. Stevens, N. Brose, J. Rizo, C. Rosenmund, and T.C. Sudhof. 2001. Synaptotagmin I functions as a calcium regulator of release probability. Nature. 410:4149.[CrossRef][Medline]
Hayashi, T., H. McMahon, S. Yamasaki, T. Binz, Y. Hata, T.C. Südhof, and H. Niemann. 1994. Synaptic vesicle membrane fusion complex: action of clostridial neurotoxins on assembly. EMBO J. 13:50515061.[Abstract]
Hua, S.-Y., and M.P. Charlton. 1999. Activity-dependent changes in partial VAMP complexes during neurotransmitter release. Nat. Neurosci. 2:10781083.[CrossRef][Medline]
Margittai, M., D. Fasshauer, S. Pabst, R. Jahn, and R. Langen. 2001. Homo- and heterooligomeric SNARE complexes studies by site-directed spin labeling. J. Biol. Chem. 276:1316913177.
McNew, J.A., T. Weber, D.M. Engelman, T.H. Söllner, and J.E. Rothman. 1999. The length of the flexible SNAREpin juxtamembrane region is a critical determinant of SNARE-dependent fusion. Mol. Cell. 4:415421.[Medline]
McNew, J.A., T. Weber, F. Parlati, R.J. Johnston, T.J. Melia, T.H. Söllner, and J.E. Rothman. 2000b. Close is not enough: SNARE-dependent membrane fusion requires an active mechanism that transduces force to membrane anchors. J. Cell Biol. 150:105117.
Munson, M., X. Chen, A.E. Cocina, S.M. Schultz, and F.M. Hughson. 2000. Interactions within the yeast t-SNARE Sso1p that control SNARE complex assembly. Nat. Struct. Biol. 7:894902.[CrossRef][Medline]
Nickel, W., T. Weber, J.A. McNew, F. Parlati, T.H. Söllner, and J.E. Rothman. 1999. Content mixing and membrane integrity during membrane fusion driven by pairing of isolated v-SNAREs and t-SNAREs. Proc. Natl. Acad. Sci. USA. 96:1257112576.
Parlati, F., T. Weber, J.A. McNew, B. Westermann, T.H. Söllner, and J.E. Rothman. 1999. Rapid and efficient fusion of phospholipid vesicles by the alpha-helical core of a SNARE complex in the absence of an N-terminal regulatory domain. Proc. Natl. Acad. Sci. USA. 96:1256512570.
Parlati, F., O. Varmalov, K. Paz, J.A. McNew, D. Hurtado, T.H. Söllner, and J.E. Rothman. 2002. Distinct SNARE complexes mediating membrane fusion in Golgi transport based on combinatorial specificity. Proc. Natl. Acad. Sci. USA. 99:54245429.
Paumet, F., B. Brügger, F. Parlati, J.A. McNew, T.H. Söllner, and J.E. Rothman. 2001. A t-SNARE of the endocytic pathway must be activated for fusion. J. Cell Biol. 155:961968.
Poirier, M.A., J.C. Hao, P.N. Malkus, C. Chan, M.F. Moore, D.S. King, and M.K. Bennett. 1998a. Protease resistance of syntaxin-SNAP-25-VAMP complexes: Implications for assembly and structure. J. Biol. Chem. 273:1137011377.
Reim, K., M. Mansour, F. Varogueaux, H.T. McMahon, T.C. Sudhof, N. Brose, and C. Rosenmund. 2001. Complexins regulate a late step in Ca2+-dependent neurotransmitter release. Cell. 104:7181.[Medline]
Scales, S.J., Y.A. Chen, B.Y. Yoo, S.M. Patel, Y.-C. Doung, and R.H. Scheller. 2000. SNAREs contribute to the specificity of membrane fusion. Neuron. 26:457464.[Medline]
Sutton, R.B., D. Fasshauer, R. Jahn, and A.T. Brünger. 1998. Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution. Nature. 395:347353.[CrossRef][Medline]
Vale, R.D. 2000. AAA proteins: lords of the ring. J. Cell Biol. 150:F13F20.[CrossRef][Medline]
Weber, T., F. Parlati, J.A. McNew, R.J. Johnston, B. Westermann, T.H. Söllner, and J.E. Rothman. 2000. SNAREpins are functionally resistant to disruption by NSF and SNAP. J. Cell Biol. 149:10631072.
Wild, C.T., D.C. Shugars, T.K. Greenwell, C.B. McDanal, and T.J. Matthews. 1994. Peptides corresponding to a predictive -helical domain of human immunodeficiency virus type 1 gp41 are potent inhibitors of virus infection. Proc. Natl. Acad. Sci. USA. 91:97709774.
Xu, T., B. Rammner, M. Margittai, A.R. Artalejo, E. Neher, and R. Jahn. 1999. Inhibition of SNARE complex assembly differentially affects kinetic components of exocytosis. Cell. 99:713722.[Medline]
Related Article