Article |
Address correspondence to Daniel A. Portnoy, Department of Molecular and Cell Biology, 401 Barker Hall, University of California, Berkeley, CA 94703-3202. Tel.: (510) 643-3925. Fax: (510) 643-6791. E-mail: portnoy{at}uclink4.berkeley.edu
![]() |
Abstract |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Key Words: cytolysins; phagosome; virulence; molecular evolution; macrophages
![]() |
Introduction |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
The capacity of L. monocytogenes to escape from an acidic phagosomal compartment is largely mediated by listeriolysin O (LLO),* an essential determinant of L. monocytogenes pathogenesis (Portnoy et al., 1992a). LLO is a member of a family of cholesterol-dependent cytolysins (CDCs) secreted by a large number of pathogenic gram-positive bacteria (Bayley, 1997). Other examples include streptolysin O, perfringolysin O (PFO), and pneumolysin. LLO is the only CDC that is made by an intracellular pathogen and has a pronounced acidic pH optimum. Although replacement of LLO with PFO complements for secreted cytolytic activity, it does not restore virulence (Jones and Portnoy, 1994a). L. monocytogenes secreting PFO are somewhat defective in escaping from a vacuole, but, strikingly, kill the host cell subsequent to entry into the cytosol. The cytotoxicity of PFO is due in part to the lack of a PEST-like sequence in PFO, which targets LLO for phosphorylation and/or degradation in the cytosol (Decatur and Portnoy, 2000). However, bacteria synthesizing PFO containing the PEST-like sequence are only partially restored in virulence. Unlike LLO, PFO does not have a pronounced acidic pH optimum, but rather is active at both acidic and neutral pHs (Portnoy et al., 1992b). We hypothesized that the acidic pH optimum of LLO is important for either efficient lysis of the phagosome and/or lack of toxicity in the cytosol.
The crystal structure of a soluble monomeric form of PFO has revealed a four domain protein with its COOH-terminal domain 4 mediating binding to cholesterol-rich membranes (Rossjohn et al., 1997). Subsequent to membrane binding, PFO oligomerizes into a large complex, containing up to 50 individual monomers, that forms large pores in the target membrane (Shepard et al., 2000). The structure of LLO awaits solution, but based on the overall similarity in sequence and function to PFO, it is reasonable to assume conservation in the relation of sequences to functions. However, because it has no direct correlate, the biochemical basis of LLO's acidic pH optimum cannot be inferred from the structure of PFO. Further, the role of LLO's acidic pH optimum in pathogenesis is not known. In this study, we identified a single residue in LLO that increased its activity at a neutral pH, making its activity profile similar to that of PFO. We then evaluated how this mutation affected L. monocytogenes pathogenesis.
![]() |
Results |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
|
The L461T mutation in LLO reduces virulence
Having established that the L461T mutation conferred greater activity on purified LLO at a neutral pH, we introduced the mutation onto the chromosome of L. monocytogenes by allelic exchange. The resulting strain, DP-L4017, was used for further studies. The mutation had no effect on bacterial growth in vitro (unpublished data). Supernatant fluid derived from cultures of LLO L461T bacteria had a quantity of LLO similar to the wild type and had hemolytic activities at pH 5.5 and 7.4 similar to those of the purified proteins from E. coli (unpublished data).
The capacity of the LLO L461T mutant to grow in animals was evaluated by the lethal dose-50 (LD50) in the mouse listeriosis model. In BALB/c mice, the LD50 of the LLO L461T mutant was >3 x 106 as compared with an LD50 of 13 x 104 for wild-type bacteria (Table I). These data suggest that LLO pH dependence contributes to the in vivo growth of L. monocytogenes.
|
Previous studies have indicated that preventing acidification of the phagosome with the vacuolar proton ATPase inhibitor bafilomycin A1 limits L. monocytogenes escape to the cytosol (Conte et al., 1996; Beauregard et al., 1997). We reasoned that escape of the LLO L461T mutant might not be affected by bafilomycin A1 treatment because its cytolysin was active at a neutral pH. However, when bafilomycin A1 was added to the macrophages, both the mutant and the wild-type bacteria escaped less efficiently (Table I). When bafilomycin A1 was present throughout the assay, both strains escaped with about a third of the efficiency of untreated controls.
Because treatment with bafilomycin A1 prevented escape of the LLO L461T mutant as well as the wild-type bacteria, it would appear that the additional activity of the mutant at a neutral pH does not eliminate the requirement for phagosome acidification. Therefore, we measured the pH of bacterium-containing phagosomes using a pH-sensitive fluid-phase fluorescent dye to determine if the LLO L461T mutant altered the phagosomal maturation process. We found that phagosomes containing the LLO L461T mutant reached an average minimum pH of 5.5 ± 0.3 before perforation, similar to that of the wild type, which reached a minimum mean pH of 5.7 ± 0.2 (Table I). We concluded that the LLO L461T mutation had no effect on phagosomal acidification or escape, and that phagosomal acidification was necessary for the escape of the LLO L461T mutant as well as for wild type. Therefore, it is unlikely that the LLO L461T mutant's virulence defect reflects a reduced ability to escape from phagosomes or an effect on phagosome maturation. The defect is likely due to the alteration of a different part of the pathogenic life cycle.
L. monocytogenes LLO L461T damages host cell membranes
Because the LLO L461T mutant had no defect in phagosomal escape, we next examined the capacity of the bacteria to grow in host cells using a quantitative tissue culture assay (Portnoy et al., 1988). In this assay, adding the antibiotic gentamicin to the culture medium kills extracellular bacteria but has no measurable effect on the growth of intracellular wild-type bacteria. Between 2 and 5 h after infection, the LLO L461T mutant grew well within J774 macrophages with an average apparent doubling time of 58 ± 8 min, slightly longer than the wild-type doubling time of 42 ± 4 min (Fig. 2 A). Strikingly, between 5 and 8 h after infection, the LLO L461T mutant grew with a nearly twofold longer average apparent doubling time (159 ± 30 min compared with the wild-type doubling time of 83 ± 8 min). Additionally, the LLO L461T mutant did not grow to as high a maximum number of bacteria.
|
For both wild-type and LLO L461T bacteria, the drop in colony-forming units was only observed when gentamicin was present in the assay medium (unpublished data). When host membranes become permeabilized during a cell culture infection, gentamicin present in the culture medium enters the host cell and kills intracellular bacteria (Brundage et al., 1993; Decatur and Portnoy, 2000). Therefore, the gentamicin-dependent drop in numbers of intracellular bacteria suggested that host membranes had been permeabilized. Because the LLO L461T ActA mutant died earlier than the wild-type LLO
ActA strain, and because this occurred in a gentamicin-dependent manner, we hypothesized that the greater activity of LLO L461T at a neutral pH led to earlier permeabilization of the host cell membrane. If this hypothesis were true, damage could be monitored by detecting release of the host cell enzyme lactate dehydrogenase (LDH) from the cytosol of the J774 cells into the culture medium. During a 7 h infection with wild-type bacteria, very little LDH was released either in the presence or absence of gentamicin (Fig. 2 C). In the absence of gentamicin, both the LLO L461T and LLO L461T
ActAinfected cells released nearly 100% of their LDH, indicating a major disruption of the host plasma membrane. Interestingly, when J774 cells were incubated in the constant presence of gentamicin, very little LDH was released during infection by any strain. Presumably, permeabilization of the cell allowed the influx of gentamicin, which then killed the intracellular bacteria (Fig. 2 B) and prevented further permeabilization and LDH release. When gentamicin was removed after 2 h, only the J774 cells infected with the LLO L461T
ActA mutant released high quantities of LDH. When a monoclonal antibody that neutralizes LLO activity (Edelson and Unanue, 2001) was added extracellularly to the J774 cells, there was no effect on LDH release, indicating that toxicity is mediated by intracellular LLO (unpublished data).
A more sensitive method to test the integrity of the plasma membrane uses the membrane-impermeant dye propidium iodide. When membrane integrity is compromised, the dye enters the cell and increases its fluorescence upon binding cellular DNA. Staining can be measured by flow cytometry. After infection with the wild-type bacteria, most macrophage host cells still excluded the dye (Fig. 3 B). In contrast, infection with the LLO L461T mutant led to permeabilization of about half of the macrophages (Fig. 3 C), and infection with the LLO L461T ActA mutant permeabilized most of the macrophages (Fig. 3 E).
|
|
|
![]() |
Discussion |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
LLO is unique among the CDCs in that its activity is normally repressed at a neutral pH. The biochemical basis of the LLO pH optimum is not understood; it could reflect pH sensitivity in membrane binding, oligomerization, and/or pore formation. It was surprising that a single amino acid change, from leucine to threonine, could profoundly increase the hemolytic activity of LLO at a neutral pH. Neither leucine nor threonine alone contains properties that would suggest sensitivity to pH. The alteration from a nonpolar leucine to a polar threonine residue offers the potential of an additional hydrogen bond and a reduction in hydrophobicity, but in the absence of a detailed structure for LLO, it is difficult to determine the influence of these changes. However, if we assume a general structural similarity to PFO, because LLO and PFO are 42% identical (Rossjohn et al., 1997), then the L461T mutation is located on an outer loop of the fourth domain. Domain 4 has been implicated in both membrane binding and oligomerization (Nakamura et al., 1995; Bayley, 1997). Therefore, it is possible that the mutation may alter interactions with the target lipid bilayer or with other monomers in a pH-sensitive fashion. Also, there is evidence that domain 4 must undergo significant movement relative to the other domains when the monomer enters the lipid bilayer (Gilbert et al., 1999). In particular, the transmembrane hairpins of domain 3 have been proposed to pack with domain 4 when inserted in the membrane. The mutation of leucine 461 to threonine might affect intramolecular interactions in a manner that would make the cytolysin less sensitive to pH. The precise effect of the L461T mutation awaits additional biochemical analysis.
There are numerous examples of pathogens exploiting acidified compartments via pH-dependent proteins, including the viral hemagglutinins (Hernandez et al., 1996) and bacterial and protozoal toxins (Ley et al., 1990; Falnes and Sandvig, 2000). These precedents led us to hypothesize that the acidic optimum of LLO provides a mechanism to coordinate optimal pore-forming activity with maturation of the phagosome to allow efficient phagosomal escape, and any perturbation of this coordination would result in less efficient escape. Consistent with this hypothesis, a number of studies have shown that bafilomycin A1, a pharmacological inhibitor of phagosomal acidification, blocks the escape of L. monocytogenes from a phagosome (Conte et al., 1996; Beauregard et al., 1997). Therefore, it was surprising to find that the bacteria synthesizing a mutant LLO more active at a neutral pH escaped from the phagosome with a similar efficiency and at the same pH as wild-type bacteria. These data suggest that though acidification of the phagosome is important for bacterial escape, the pH sensitivity is not manifested through LLO activity. Given that bafilomycin A1 blocks maturation of endosomes (van Weert et al., 1995), these data suggest that a pH-dependent process other than LLO activity is involved in vacuolar perforation and escape.
Although the precise mechanism of vacuolar escape is not understood, there is recent evidence that CDCs, including LLO, may act as mediators of protein delivery to the host cytosol, similarly to the gram negative type III secretion system (Sibelius et al., 1996; Wadsworth and Goldfine, 1999; Madden et al., 2001). If indeed LLO acts to mediate cytosolic delivery from the phagosome, it is possible that the acid dependence is required for activation of some other pH-sensitive bacterial effector. At least one other L. monocytogenes virulence factor, a broad-range phospholipase C, is released upon vacuolar acidification (Marquis and Hager, 2000), so it is possible that LLO simply needs to form a pore to allow the transfer of the pH-sensitive effector to the cytosol to mediate vesicular escape. Another possibility is that a host factor involved in the escape process requires acidification to be activated or localized to the phagosome.
The most striking phenotype exhibited by L. monocytogenes expressing LLO L461T was the increased damage inflicted on the host cells' membranes. The simplest explanation for this observation is that LLO L461T is active within the neutral pH of the cytosol and passes a threshold at which the number of pores formed by the toxin overwhelms the host cell's membrane repair mechanisms (Walev et al., 2001). Alternately, it is possible that the mutation may lead to host cell damage through an unknown mechanism. However, our data clearly indicate that bacteria synthesizing LLO L461T permeabilize J774 host cells as early as 5 h after infection, whereas permeabilization by the wild type requires 8 h. It has been estimated that one bacterium secretes one LLO molecule per minute (Villanueva et al., 1995). Based on this rate and the doubling times for each strain, we calculated that at the time of permeabilization, the host cell is confronted with the production of 35 LLO L461T molecules per minute, enough monomers to assemble approximately one pore. In contrast, the wild-type bacteria are producing 420 LLO per minute when they permeabilize the host cell after 8 h of growth. These calculations indicate that bacteria synthesizing LLO L461T permeabilized the host cell with about one twelfth the production rate of wild type. This difference indicates that the unique acidic activity optimum of wild-type LLO enables L. monocytogenes to produce at least 10 times the number of progeny per infected host cell before significant host damage occurs.
Many factors contribute to the host cell's ability to deal with the presence of LLO within the cytosol. In light of studies suggesting that host cytosolic proteases cleave LLO (Villanueva et al., 1995; Decatur and Portnoy, 2000), an alternate explanation for the greater toxicity of LLO L461T is that it is less susceptible to proteolytic degradation. We observed an approximately twofold higher concentration of LLO L461T in the cytosol of J774 host cells, yet found that equal quantities of LLO were produced in vitro. Perhaps the greater concentration of LLO L461T reflected the fact that the cytolysin entered membranes more readily in the neutral pH of the cytosol where it was protected from proteolytic degradation, as has been shown for other CDCs (Nakamura et al., 1995). Regardless of the precise mechanism leading to the greater concentration of intracellular LLO L461T, we found that intracellular concentrations of LLO did not correlate with membrane permeabilization. A merodiploid LLO strain producing twice as much LLO was no more cytotoxic than the wild type, suggesting that a twofold increase in LLO concentration alone does not affect membrane damage under these conditions. However, a merodiploid strain synthesizing both the LLO L461T and the wild-type LLO acted similarly to the LLO L461T strain in the plaquing assay, producing gentamicin-sensitive plaques (unpublished data), suggesting that the mutant's cytotoxic phenotype is dominant over the wild type. These data suggest that the increased host membrane damage was due to the 10-fold greater activity of LLO L461T at a neutral pH and not simply intracellular LLO concentration.
The results of this study showed that L. monocytogenes expressing LLO L461T was 100-fold less virulent than the wild type. We hypothesize that the increased damage to the plasma membrane observed in tissue culture correlates with the defect in virulence in vivo. Additionally, we found that the LLO L461T grew less well in immunologically naive mice as early as 12 h after infection (unpublished data). Thus, the adaptive immune response probably plays little role in mediating the defect of the mutant bacteria. Instead, the defect is more likely to reflect the action of the innate immune response or a more basic defect in the bacterial life cycle in vivo. We can envision a number of scenarios that may lead to the mutant's virulence defect. One possibility is that a damaged cell no longer provides the advantages of an intracellular lifestyle, whether it is caused by the loss of access to nutrients, reduced actin-based motility, a loss of protection from humoral defenses, or another unknown mechanism. However, because the mutant grows and spreads normally at low gentamicin concentrations, a more likely possibility is that the mutant elicits a greater inflammatory response at the foci of infections due to greater cytotoxicity, thereby recruiting a greater number of leukocytes to the focus of infection. The hemolytic activity of LLO causes the production of inflammatory cytokines, leading to activation and chemotaxis of neutrophils and monocytes, which are largely responsible for containing the bacteria (Unanue, 1997; Kayal et al., 1999; Sibelius et al., 1999).
The results of this study support the concept that L. monocytogenes has evolved to minimize harm to its host cell. To achieve maximal virulence, the bacteria must maintain an equilibrium between producing a molecule that is cytolytic enough to mediate escape from the vesicle, yet not overly toxic to infected host cells. We hypothesize that L. monocytogenes has acquired a pathoadaptive mutation (Sokurenko et al., 1999) converting the primordial residue 461 to a leucine. This mutation is found solely in pathogenic Listeria and converts LLO from a toxin appropriate for an extracellular pathogen to that of an intracellular pathogen. Thus, LLO is highly active where it needs to be, in the acidic phagosome, yet relatively inactive in the neutral pH of the cytosol. Together with previously published data that LLO contains a PEST-like sequence that also reduces its toxicity (Decatur and Portnoy, 2000), our data imply that L. monocytogenes has evolved multiple failsafe mechanisms to regulate the activity of LLO, a molecule that has the potential to destroy its intracellular niche. Indeed, it has been hypothesized that the host cell uses these same acidic properties of the endocytic pathway to compartmentalize the activity of its own potentially hazardous lysosomal enzymes (Mellman et al., 1986).
![]() |
Materials and methods |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
L. monocytogenes was grown in 3 ml brain heart infusion broth (BHI; Becton Dickinson) slanted without agitation in 15 ml conical tubes at 30°C overnight, unless otherwise noted. All E. coli strains were grown in Luria-Bertani broth (LB; Becton Dickinson) at 37°C shaking, unless otherwise noted. All tissue culture cells were grown in DME (GIBCO BRL), containing 7.5% heat-deactivated FBS (Hy-Clone) and 2 mM glutamine (DME; GIBCO BRL), at 37°C and 5% CO2, unless otherwise noted. All chemicals were purchased from Sigma-Aldrich, unless otherwise noted.
Sequences
The GenBank/EMBL/DDBJ accession nos. for the proteins examined in this study are the following: LLO, M29030; PFO, M36704; ivanolysin O, X60461; seeligeriolysin O, X60462; streptolysin O, M18638; pneumolysin, X52474; cereolysin, D21270; alveolysin, M62709; suilysin, Z36907; and pyolysin, U84782.
Cloning
Construction of the LLO expression vector.
DNA and protein analysis was performed using MacVector software (Genetics Computer Group). The region of hly coding for mature LLO was amplified by PCR, with the primers and templates described in Table II, using Vent polymerase (New England Biolabs, Inc.) to introduce a six histidine tag. The amplified fragment was then cut with restriction enzymes and ligated into pET29b (Novagen). This plasmid and all other plasmids were initially cloned in E. coli strain XL-1 Blue and then transferred into E. coli expression strain BL21(DE3), unless otherwise noted, to yield strain DP-3570.
|
Construction of the domain chimeras, subdomain chimeras, and single amino acid mutation expression vectors.
The fourth domain of LLO was replaced by PFO domain 4 using splicing by overlap extension PCR (Horton et al., 1990) with the components and restriction sites described in Table II. The subdomain chimeras and the single amino acid mutations indicated in Fig. 1 were constructed by modifying p3570 with the protocol published in the QuickchangeTM site-directed mutagenesis kit (Stratagene) and the primers listed in Table II.
Hemolytic activity screening of recombinant proteins
E. coli expression strains were grown overnight in LB containing 30 µg/ml kanamycin (LB-KAN). 400 µl of the overnight culture was added to 10 ml LB-KAN and grown for 1.5 h, and then 1 mM IPTG was added. This culture was incubated at 30°C, shaking for 3 h. Cultures were pelleted and then resuspended in 1 ml storage buffer (140 mM sodium chloride, 4 mM potassium chloride, 10 mM sodium phosphate, 0.5 mM DTT, pH 6.0) with 1 mM PMSF. The samples were sonicated on ice and cleared by centrifugation.
The quantitative assay was performed in a 96-well V-bottom styrene plate (Corning Inc.) with either neutral hemolysis buffer (35 mM sodium phosphate, 125 mM sodium chloride, 0.5 mg/ml BSA, pH 7.4, using acetic acid) or acidic hemolysis buffer (same as neutral hemolysis buffer but pH 5.5). Samples were serially diluted, and then 0.5% sheep red blood cells (HemoStat Laboratories) were added to each well. The plate was incubated, shaking at 37°C, and then pelleted in the V-bottom. Supernatant was transferred from the V-bottom plate into equivalent locations in a flexible polyvinyl chloride flat bottom 96-well plate (Becton Dickinson), and the absorbance at 450 nm for each well was measured (Spectramax340) and analyzed with SoftMax Pro v1.2 software (Molecular Devices Corp.). Hemolytic units were defined as the dilution of the sample at which 50% of the sheep red blood cells had been lysed.
Overexpression and purification of 6x histagged LLO proteins and 6x histagged PFO from E. coli
Recombinant strains were grown, shaking at 37°C, in LB-KAN to stationary phase. 20 ml of this culture was inoculated into 1 liter of LB-KAN and incubated, shaking at 30°C, for 90 min. Expression was induced by the addition of 1 mM IPTG, and the culture was incubated, shaking at 30°C, for 6 h. The bacterial pellet was harvested by centrifugation, resuspended in 40 ml cold lysis buffer (50 mM sodium phosphate, pH 8.0, 1 M sodium chloride, 20 mM imidazole, 10 mM 2-mercaptoethanol, 1 mM PMSF), and lysed in a French pressure cell at 12,000 psi. The lysate was centrifuged for 20 min at 17,000 g. The supernatant was collected and mixed into 5 ml Ni-NTA resin (QIAGEN) equilibrated in lysis buffer. The slurry was stirred at 4°C for 60 min to bind his-tagged protein to the resin. To remove unbound protein, the resin was packed into a column and washed with lysis buffer by dropwise gravity flow until UV absorbance of the eluate reached baseline, and then it was washed with wash buffer (lysis buffer, pH 6.0, 10% glycerol, 0.1% Tween 20). Washed resin was removed from the column, resuspended in elution buffer (lysis buffer, pH 6.0, and 800 mM imidazole), and incubated 10 min on ice, after which the supernatant was collected. This procedure was performed twice, yielding 6 ml eluate. Eluate was dialyzed in cassettes (Pierce Chemical Co.) within autoclaved storage buffer (lysis buffer, pH 6.0, with 1 mM EDTA). Both the Bradford method and UV280 absorbance determined protein concentrations. The procedure yielded 25 mg protein per liter starting culture. Aliquots not used immediately were stored in storage buffer with 50% glycerol at -80°C.
Allelic exchange of LLO L461T
To introduce the LLO L461T mutation onto the 10403S chromosome, a DNA fragment was produced by splicing by overlap extension PCR, using the primers, templates, and restriction enzymes in Table II, and then ligated into the temperature-sensitive plasmid vector pKSV7. Allelic exchange was performed as described previously (Camilli et al., 1993). Strains were verified initially by detecting the loss of an Nhe1 site in a chromosomal PCR product containing the mutation.
Animal studies
LD50 by intravenous infection was established as previously described using BALB/c mice (Portnoy et al., 1988).
Phagosomal escape assay
The percentage of bacteria that had escaped from the phagosome was determined by evaluating the presence of F-actincoated bacteria within the macrophage, similar to an experiment previously described (Jones and Portnoy, 1994a). C57/BL6 BMs in DME, 10% FBS, with or without 0.5 µM bafilomycin A1 (Calbiochem), on a coverslip were infected for 15 min, resulting in a bacterium within 1/10 macrophages. Macrophages were washed with Ringer's buffer (5 mM NaCl, 5 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 2 mM NaH2PO4, 10 mM Hepes, 10 mM glucose, pH 7.2) and 25 µg/ml gentamicin was added. At 120 min after infection, the macrophages were fixed for 15 min with cytoskeletal fixative (40 mM Hepes, 10 mM EGTA, 0.5 mM EDTA, 5 mM MgSO4, 33 mM potassium acetate, 0.02% sodium azide, 5% polyethylene glycol 400, 4% paraformaldehyde), washed, permeabilized with PBS, containing 2% goat serum and 0.3% Triton X-100, and stained with Texas redphalloidin (Molecular Probes) and DAPI (Molecular Probes). A total of 50 macrophages harboring bacteria were examined for each bacterial strain in each of four experiments.
The determination of phagosomal pH was performed essentially as previously described (Beauregard et al., 1997) with the following modifications. In brief, fluid-phase fluorescein dextran, molecular weight 10,000 (Molecular Probes), was added to the bacteria-containing media used to infect macrophages. Phagosomes containing both 10-kD fluorescein dextran and bacteria were photographed every 30 s with a Quantix cooled charge-couple device camera (Photometrics) through fluorescent microscopy using a Nikon TE300 inverted microscope (Nikon), with phase-contrast and excitation wavelengths 485 and 440 nm and emission measurement at 520 nm. Images and the 485:440 ratio were collected until perforation was indicated by loss of dye from the vacuole. The 485:440 ratio measured just before perforation was compared with a standard curve to establish pH, as described in the published methods.
Cytotoxicity assays
Growth in J774 macrophage-like cells.
Intracellular growth of L. monocytogenes was performed as previously described (Jones and Portnoy, 1994b).
Flow cytometry.
Flow cytometry was performed on cultures of BMØs from CD-1 mice as previously described (Portnoy et al., 1988). BMØ were chosen for this assay because infected J774 are difficult to remove from tissue culture dishes without causing plasma membrane damage, whereas BMØ lift from the dish when incubated at 4°C. 106 macrophages were plated on 60-mm Lab-tek nontissue culture dishes (Fisher Scientific) overnight in bone marrow macrophage media (DME, 20% heat-deactivated FBS, 30% L cell supernatant containing CSF-1 in DME, 2 mM glutamine, 1 mM pyruvate, 0.1% 2-mercaptoethanol). Monolayers were infected with 107 washed bacteria for 30 min resulting in at least one bacterium per cell. 60 min after infection, 50 µg/ml gentamicin was added. 3 h after infection, the cell monolayer was washed to remove the gentamicin, and then fresh medium was added to the dish. 7 h after infection, medium from each culture was collected. 4°C PBS was then added and the dish was stored at 4°C for 30 min. Release of macrophages from the dish was monitored by microscopy. The macrophage-containing PBS was added to the previously removed media and centrifuged at 4°C. The pellet was washed with 4°C PBS, 10% FBS. Cell pellets were resuspended in PBS (10% FBS) and passed through a 70-µm cell strainer (Becton Dickinson). 1 µg propidium iodide (Molecular Probes) was added to each sample. Samples were analyzed with a flow cytometer (EPICS XL-MCL; Beckman Coulter).
LDH release assay.
LDH release assays were performed using the Cytotox 96® nonradioactive cytotoxicity assay (Promega), according to manufacturer's instructions and methods described previously (Decatur and Portnoy, 2000), with 2 x 104 J774 cells per well infected to achieve at least one bacterium per cell. Neutralizing anti-LLO monoclonal antibodies were supplied by Brian Edelson and Emil Unanue (Washington University School of Medicine, St. Louis, MO).
Intracellular LLO analysis
Intracellular levels of LLO were studied with previously established methods (Moors et al., 1999) and the following modifications. In brief, J774 cells were infected with L. monocytogenes strains for 30 min and then washed, and 50 µg/ml gentamicin was added at 60 min. 4 h after infection, methionine-starved cells were pulsed with [35S]methionine (NEN Life Science Products) for 1 h. At 5 h, macrophages were lysed, LLO was immunoprecipitated, and one half of the sample was subjected to SDS-PAGE for autoradiography and the other half run for analysis on a Phosphorimager 445 SI (Molecular Dynamics) and analyzed using Imagequant software (Molecular Dynamics). Monoclonal anti-LLO antibodies were supplied by Pascale Cossart (Institute Pasteur, Paris, France). The relative number of bacteria in each assay was established by lysing the infected J774 on coverslips in dishes processed in tandem with the radiolabeled dishes. Lysate was subsequently plated on LB-agar plates to determine colony-forming units.
Plaque assay
Plaquing assays within L2 cell monolayers were performed as described previously (Sun et al., 1990), with modifications to the methods of measurement (Skoble et al., 2000). In brief, L2 cells were grown to confluency in six-well tissue culture dishes and then infected with bacteria for 1 h. Subsequently, DME-agar containing gentamicin was added and plaques were grown for 3 d. Living cells were visualized by adding on day 3 an additional DME-agar overlay containing neutral red (GIBCO BRL) and incubating overnight.
![]() |
Footnotes |
---|
M.M. Gedde is currently at IntraBiotics Pharmaceuticals, 1245 Terra Bella Ave., Mountain View, CA 94043.
* Abbreviations used in this paper: BMØ(s), bone marrowderived macrophage(s); CDC(s), cholesterol-dependent cytolysin(s); LB, Luria-Bertani broth; LB-KAN, Luria-Bertani broth with 30 µg/ml kanamycin; LD50, lethal dose-50; LDH, lactate dehydrogenase; LLO, listeriolysin O; PFO, perfringolysin O.
![]() |
Acknowledgments |
---|
This work was supported by National Institutes of Health grant AI27655 (D.A. Portnoy) and AI35950 (J.A. Swanson). M. Gedde was a Howard Hughes Medical Institute postdoctoral physician fellow.
Submitted: 18 January 2002
Revised: 4 February 2002
Accepted: 4 February 2002
![]() |
References |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Beauregard, K.E., K.D. Lee, R.J. Collier, and J.A. Swanson. 1997. pH-dependent perforation of macrophage phagosomes by listeriolysin O from Listeria monocytogenes. J. Exp. Med. 186:11591163.
Brundage, R.A., G.A. Smith, A. Camilli, J.A. Theriot, and D.A. Portnoy. 1993. Expression and phosphorylation of the Listeria monocytogenes ActA protein in mammalian cells. Proc. Natl. Acad. Sci. USA. 90:1189011894.[Abstract]
Conte, M.P., G. Petrone, C. Longhi, P. Valenti, R. Morelli, F. Superti, and L. Seganti. 1996. The effects of inhibitors of vacuolar acidification on the release of Listeria monocytogenes from phagosomes of Caco-2 cells. J. Med. Microbiol. 44:418424.[Abstract]
Decatur, A.L., and D.A. Portnoy. 2000. A PEST-like sequence in listeriolysin O essential for Listeria monocytogenes pathogenicity. Science. 290:992995.
Falnes, P.O., and K. Sandvig. 2000. Penetration of protein toxins into cells. Curr. Opin. Cell Biol. 12:407413.[CrossRef][Medline]
Gedde, M.M., D.E. Higgins, L.G. Tilney, and D.A. Portnoy. 2000. Role of listeriolysin O in cell-to-cell spread of Listeria monocytogenes. Infect. Immun. 68:9991003.
Gilbert, R.J., J.L. Jimenez, S. Chen, I.J. Tickle, J. Rossjohn, M. Parker, P.W. Andrew, and H.R. Saibil. 1999. Two structural transitions in membrane pore formation by pneumolysin, the pore-forming toxin of Streptococcus pneumoniae. Cell. 97:647655.[Medline]
Horton, R.M., Z.L. Cai, S.N. Ho, and L.R. Pease. 1990. Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction. Biotechniques. 8:528535.[Medline]
Jones, S., and D.A. Portnoy. 1994a. Characterization of Listeria monocytogenes pathogenesis in a strain expressing perfringolysin O in place of listeriolysin O. Infect. Immun. 62:56085613.[Abstract]
Kayal, S., A. Lilienbaum, C. Poyart, S. Memet, A. Israel, and P. Berche. 1999. Listeriolysin O-dependent activation of endothelial cells during infection with Listeria monocytogenes: activation of NF-kappa B and upregulation of adhesion molecules and chemokines. Mol. Microbiol. 31:17091722.[CrossRef][Medline]
Ley, V., E.S. Robbins, V. Nussenzweig, and N.W. Andrews. 1990. The exit of Trypanosoma cruzi from the phagosome is inhibited by raising the pH of acidic compartments. J. Exp. Med. 171:401413.[Abstract]
Marquis, H., and E.J. Hager. 2000. pH-regulated activation and release of a bacteria-associated phospholipase C during intracellular infection by Listeria monocytogenes. Mol. Microbiol. 35:289298.[CrossRef][Medline]
Moors, M.A., B. Levitt, P. Youngman, and D.A. Portnoy. 1999. Expression of listeriolysin O and ActA by intracellular and extracellular Listeria monocytogenes. Infect. Immun. 67:131139.
Portnoy, D.A., P.S. Jacks, and D.J. Hinrichs. 1988. Role of hemolysin for the intracellular growth of Listeria monocytogenes. J. Exp. Med. 167:14591471.[Abstract]
Portnoy, D.A., R.K. Tweten, M. Kehoe, and J. Bielecki. 1992b. Capacity of listeriolysin O, streptolysin O, and perfringolysin O to mediate growth of Bacillus subtilis within mammalian cells. Infect. Immun. 60:27102717.[Abstract]
Shepard, L.A., O. Shatursky, A.E. Johnson, and R.K. Tweten. 2000. The mechanism of pore assembly for a cholesterol-dependent cytolysin: formation of a large prepore complex precedes the insertion of the transmembrane beta-hairpins. Biochemistry. 39:1028410293.[CrossRef][Medline]
Sibelius, U., T. Chakraborty, B. Krogel, J. Wolf, F. Rose, R. Schmidt, J. Wehland, W. Seeger, and F. Grimminger. 1996. The listerial exotoxins listeriolysin and phosphatidylinositol-specific phospholipase C synergize to elicit endothelial cell phosphoinositide metabolism. J. Immunol. 157:40554060.[Abstract]
Sibelius, U., E.C. Schulz, F. Rose, K. Hattar, T. Jacobs, S. Weiss, T. Chakraborty, W. Seeger, and F. Grimminger. 1999. Role of Listeria monocytogenes exotoxins listeriolysin and phosphatidylinositol-specific phospholipase C in activation of human neutrophils. Infect. Immun. 67:11251130.
Skoble, J., D.A. Portnoy, and M.D. Welch. 2000. Three regions within ActA promote Arp2/3 complex-mediated actin nucleation and Listeria monocytogenes motility. J. Cell Biol. 150:527538.
Skoble, J., V. Auerbuch, E.D. Goley, M.D. Welch, and D.A. Portnoy. 2001. Pivotal role of VASP in Arp2/3 complex-mediated actin nucleation, actin branch-formation, and Listeria monocytogenes motility. J. Cell Biol. 155:89100.
Sun, A., A. Camilli, and D.A. Portnoy. 1990. Isolation of Listeria monocytogenes small-plaque mutants defective for intracellular growth and cell-to-cell spread. Infect. Immun. 58:37703778.[Medline]
Tilney, L.G., and D.A. Portnoy. 1989. Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes. J. Cell Biol. 109:15971608.[Abstract]
van Weert, A.W.M., K.W. Dunn, H.J. Geuze, F.R. Maxfield, and W. Stoorvogel. 1995. Transport from late endosomes to lysosomes, but not sorting of integral membrane proteins in endosomes, depends on the vacuolar proton pump. J. Cell Biol. 130:821834.[Abstract]
Villanueva, M.S., A.J. Sijts, and E.G. Pam. 1995. Listeriolysin is processed efficiently into an MHC class I-associated epitope in Listeria monocytogenes-infected cells. J. Immunol. 155:52275233.[Abstract]
Wadsworth, S.J., and H. Goldfine. 1999. Listeria monocytogenes phospholipase C-dependent calcium signaling modulates bacterial entry into J774 macrophage-like cells. Infect. Immun. 67:17701778.
Walev, I., S.C. Bhakdi, F. Hofmann, N. Djonder, A. Valeva, K. Aktories, and S. Bhakdi. 2001. Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O. Proc. Natl. Acad. Sci. USA. 98:31853190.
Related Article