Article |
Address correspondence to Michael Sendtner, Institute for Clinical Neurobiology, Josef-Schneider-Str. 11, 97080 Wuerzburg, Germany. Tel.: (49) 931-201-49767. Fax: (49) 931-201-49788. email: sendtner{at}mail.uni-wuerzburg.de
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Abstract |
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Key Words: SMA; SMN; RNA transport; ß-actin; hnRNP R
The online version of this article includes supplemental material.
Abbreviations used in this paper: hnRNP, heterogeneous nuclear ribonucleoprotein; phospho-tau, phosphorylated tau protein; RRM, RNA recognition motif; SMA, spinal muscular atrophy; SMN, survival motoneuron; snRNPs, small nuclear ribonucleoproteins.
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Introduction |
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SMN plays an essential role in assembly and regeneration of spliceosomal small nuclear ribonucleoproteins (snRNPs) in all cell types (Fischer et al., 1997; Meister et al., 2002; Paushkin et al., 2002). Despite progress in the understanding of the function of SMN in these processes, very little is known about the cause of the mainly motoneuron-specific pathology in SMA. There is no evidence that abnormalities of spliceosomal snRNP biogenesis and metabolism cause defects in mRNA splicing in motoneurons from mouse models (Jablonka et al., 2000) or SMA patients (for review see Sendtner, 2001).
Several lines of evidence support additional neuron-specific functions of SMN. Mice in which the Smn gene is specifically deleted in neurons show postnatal cell death (Frugier et al., 2000) associated with accumulation of neurofilament in motoneuron cell bodies and at the motor endplate (Cifuentes-Diaz et al., 2002). The Smn protein is localized in axons and growth cones of motoneurons, both in cell culture and in vivo (Jablonka et al., 2001; Fan and Simard, 2002). Interestingly, Smn is not colocalized with Gemin2 in axons (Jablonka et al., 2001). Because Gemin2 is an essential component of complexes that assemble snRNPs, this finding suggests that Smn might serve additional functions in axons of motoneurons. Two novel interaction partners for Smn, the highly related RNA-binding heterogeneous nuclear ribonucleoproteins R and Q (hnRNP R and hnRNP Q, respectively; Mourelatos et al., 2001), have been found to colocalize with Smn in motor axons (Rossoll et al., 2002).
Here, we report that Smn and its binding partner hnRNP R modulate axon growth. We measured survival and neurite length in isolated motoneurons from a mouse model of SMA and found a specific reduction in axon growth, but no alterations in survival or dendrite length. Moreover, neurite outgrowth is enhanced in PC12 cells overexpressing Smn and/or hnRNP R. We also show that deficiency of Smn protein leads to alterations of ß-actin protein and mRNA localization in axons and growth cones. hnRNP R associates with ß-actin mRNA, and binding of Smn to hnRNP R appears necessary for this interaction. These data indicate that Smn and hnRNP R are involved in processing and localization of ß-actin mRNA to growth cones of developing motoneurons. A defect in this function could explain the relatively high specificity of the disease for motoneurons.
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Results |
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Smn and hnRNP R promote neurite growth in PC12 cells
Because reduced Smn protein levels lead to reduced axonal growth, we examined whether overexpression of Smn or its binding partner hnRNP R affects neurite outgrowth in differentiating neuronal cells. For this purpose, we transiently transfected PC12 cells with expression constructs for wild-type and mutant Smn as well as the Smn-interacting protein hnRNP R. We also tested hnRNP R mutants lacking the Smn interaction domain, which has been identified between aa 522 and 556 (hnRNP R Smn; Mourelatos et al., 2001). Because hnRNP R, in contrast to Smn, contains RNA-binding domains, we also tested mutants lacking the RNA recognition motifs (RRM) 1 and 2 between aa 166 and 331 (Fig. 2 A; hnRNP R
RRM1,2). RRM1 and RRM2 appear to be the primary RNA-binding sites (Rossoll et al., 2002). The Smn interaction domain is highly homologous between hnRNP R and the closely related hnRNP Q (Mourelatos et al., 2001). Endogenous protein was detected with anti-hnRNP R antibody, whereas overexpressed wild-type or truncated hnRNP R forms were identified with an mAb against the NH2-terminal HA tag, but also with hnRNP R antibodies as a control. As observed previously with primary cultured motoneurons, wild-type hnRNP R colocalized with Smn in cell bodies and neuritic processes of differentiating PC12 cells (Fig. 2, BD). In contrast, HA-tagged hnRNP R
Smn was present mainly in the nucleus (Fig. 2, EG). This finding suggests that interaction with Smn influences the subcellular distribution of hnRNP R. No gross mislocalization of Smn was detectable in cell lines overexpressing hnRNP R constructs (Fig. S1, available at http://www.jcb.org/cgi/content/full/jcb.200304128/DC1).
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Reduced hnRNP R and ß-actin in Smn-deficient motor axons
Previous EM analyses showed that Smn protein is associated with axonal microtubules (Bechade et al., 1999; Pagliardini et al., 2000). We have found that Smn protein is colocalized with the RNA-binding protein hnRNP R (Rossoll et al., 2002), but not with Gemin2 in motor axons (Jablonka et al., 2001), indicating that a complex of Smn and hnRNP R might be involved in axonal RNA transport or processing (Rossoll et al., 2002). Therefore, we investigated whether distribution of ß-actin is disturbed in motoneurons from Smn-/-; SMN2 mice. In wild-type motoneurons, ß-actin staining was concentrated in distal parts of the axon (Fig. 4 A). In axons of Smn-/-; SMN2 motoneurons, ß-actin staining was very faint in growth cones and distal parts of the axon (Fig. 4 B). The isoform-specific antibody used for visualization of ß-actin recognizes the NH2 terminus (aa 215), and this epitope could be masked by posttranslational modification or actin-binding proteins. Therefore, we also performed immunostaining with anti-actin antibodies that recognize a more COOH-terminal epitope (aa 5070). Again, disturbed distribution of actin was found in the distal axon and growth cone of motoneurons isolated from Smn-/-; SMN2 embryos (Fig. 4 D).
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To investigate whether the distribution of other cytoskeletal proteins is also affected, we immunostained cultured motoneurons from Smn-/-; SMN2 and control mice with antibodies against tubulin (Fig. 4 G, ß-tubulin III) and neurofilament (Fig. 4 H, NF-M). Both proteins were present at normal levels and distribution in the axons of cultured Smn-/-; SMN2 motoneurons.
Reduced growth cone area in Smn-deficient motor axons
High power magnification of the distal axons stained with antibodies against ß-actin, tubulin (ß-tubulin III), and neurofilament (NF-M) showed a distinct reduction of growth cone size by all antibodies used (Fig. 5, A, C, and D). Staining with ß-actin, which is enriched in growth cones including the filopodia, was used for morphometric analysis. The results show a more than threefold reduction of the area covered by the axonal growth cones in Smn-/-; SMN2 motoneurons (Fig. 5 B).
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hnRNP R associates with the 3' UTR of ß-actin mRNA
It has been shown that the 3' UTR of ß-actin is important for the transport of the transcript (Kislauskis et al., 1993). A 54-bp domain called zipcode (Kislauskis et al., 1994) is bound by specific proteins that are involved in this process (Ross et al., 1997; Zhang et al., 2001; Gu et al., 2002). Therefore, we investigated whether hnRNP R or Smn can interact with the 3' UTR of ß-actin mRNA in vitro.
Full-length HA-tagged hnRNP R, hnRNP R RRM1,2, and the hnRNP R
Smn mutants, as well as HA-tagged Smn and the transcription factor c-Jun (as a negative control), were immunopurified from transfected HEK 293 cells and incubated with either the full-length 3' UTR of ß-actin mRNA or the fragment corresponding to the zipcode region (Kislauskis et al., 1994). Both in vitrotranscribed RNAs contained a poly(A)+ tail of 30 nucleotides. A full-length cDNA clone of I
B
including the 3' UTR and the poly(A)+ tail was used as a specificity control. The 3' UTR of ß-actin mRNA and also the truncated RNA, which consisted of only the zipcode region, was specifically bound by full-length hnRNP R, but neither by the truncated hnRNP R mutants nor by Smn alone or c-Jun (Fig. 7 A). No interaction was observed between hnRNP R and I
B
mRNA (Fig. 7 A). To rule out that hnRNP R interaction specifically involves the poly(A)+ tail of ß-actin mRNA, we performed similar experiments using the full-length 3' UTR of ß-actin mRNA with or without a 30-mer poly(A)+ tail. A 500-bp fragment of lysozyme mRNA, containing the COOH terminus of the ORF and part of the 3' UTR, was used as a specificity control. Specific association was observed for both constructs of ß-actin mRNA (Fig. 7 B). These data strongly indicate that hnRNP R interacts with the 3' UTR of ß-actin.
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To show association of ß-actin transcripts with hnRNP R in vivo, we performed RT-PCR of mRNA coimmunoprecipitated with hnRNP R. HA-tagged wild-type hnRNP R or the RRM and
Smn mutants were immunopurified from differentiated PC12 cell lines. Bound mRNA was isolated from immunoprecipitates, was reverse transcribed into cDNA, and RT-PCR was performed with ß-actinspecific primers. A 209-bp DNA fragment can be amplified from RNA bound to wild-type hnRNP R, but is barely visible when using RNA bound by mutant hnRNP R (Fig. 7 C). c-Junspecific primers used as a specificity control do not amplify a DNA fragment (expected size of 213 bp). Although we can clearly show association of ß-actin mRNA and hnRNP R, future work is needed to demonstrate whether this interaction is direct, or whether it involves additional proteins like ZBP1 (Zhang et al., 2001) or ZBP2 (Gu et al., 2002).
In summary, our results show that a complex of Smn and hnRNP R associates with ß-actin mRNA and translocates to axons in motoneurons. Disturbances in this process could lead to reduced ß-actin levels in growth cones, and thus to reduced axon growth in SMA.
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Discussion |
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In differentiating PC12 cells, overexpression of Smn as well as hnRNP R leads to enhanced neurite outgrowth. This effect depends on the interaction of these two proteins. They are colocalized in axons of cultured motoneurons and also in axons of motor nerves in adult mice (Rossoll et al., 2002). SMN protein has been shown to be present in a stable complex with Gemin27 (for review see Meister et al., 2002; Paushkin et al., 2002) in the nucleus within specific structures called gemini of Cajal (coiled) bodies ("gems"). Interestingly, hnRNP R has not been identified as part of this complex. Gems seem to play an important role for snRNP assembly and RNA processing (Carvalho et al., 1999; Young et al., 2000; Sleeman et al., 2001). In motoneurons, Smn is also localized in axons (Pagliardini et al., 2000; Jablonka et al., 2001; Fan and Simard, 2002), but it does not colocalize with Gemin2 (Jablonka et al., 2001), and instead colocalizes with hnRNP R in this part of the cell (Rossoll et al., 2002). Others have observed Smn in association with cytoskeletal elements in spinal dendrites and axons (Bechade et al., 1999; Pagliardini et al., 2000). Recently, cytoskeletal-based active transport of SMN containing granules in neuronal processes and growth cones has been demonstrated in transfected cultured neurons (Zhang et al., 2003). Binding of Smn to hnRNP R and localization of these proteins in motor axons (Rossoll et al., 2002) suggest that Smn could be involved in the transport of specific mRNAs in motor axons.
Increasing evidence points to the importance of RNA localization and transport within polarized cells. Sorting of defined mRNA species to distinct subcellular regions is observed in many cell types, in particular in neurons (for review see Mohr and Richter, 2001). The actin cytoskeleton plays an important role in axon initiation, growth, guidance, branching, and retraction, and also in synapse formation and stability (for review see Luo, 2002). ß-Actin protein is highly enriched in distal parts of axons and growth cones. Specific transport of ß-actin mRNA to axons contributes to this distribution (Bassell et al., 1998; Zhang et al., 1999, 2001). Our observation that axon growth is significantly impaired in Smn-/-; SMN2 motoneurons (and that ß-actin protein distribution and growth cone morphology is disturbed in these cells) points to an essential role of Smn and hnRNP R in this function. To investigate a possible direct effect on the transport of ß-actin mRNA, we performed in situ hybridization experiments to localize actin transcripts in neurites of developing PC12 cells and primary motoneurons (Fig. 6). Our results suggest that wild-type Smn levels are required for accumulation of actin mRNA in growth cones of motoneurons. Furthermore, we show that hnRNP R mutants that cannot bind RNA or Smn exert a dominant-negative effect on mRNA translocation. This corresponds to reduced neurite growth in PC12 cell lines that express hnRNP R RRM (Fig. 3 A). Our data show that the observed reduced distal ß-actin protein localization is caused, at least to some extent, by a direct effect of Smn and hnRNP R on distal actin mRNA accumulation.
It has been shown that the localization of ß-actin mRNA involves a 54-nt sequence within the 3' UTR (termed zipcode) that is both sufficient and necessary for peripheral localization (Kislauskis et al., 1994). Our findings indicate that hnRNP R can associate with full-length ß-actin mRNA as well as the zipcode region, and that this interaction does not depend on a poly(A)+ tail. Because neither the lysozyme nor the IB
control mRNAs are bound, specificity for this interaction can be anticipated. Two proteins, ZBP1 and ZBP2, which interact with the same zipcode domain of ß-actin mRNA and seem to be involved in axonal transport of ß-actin mRNA in chick forebrain (Zhang et al., 2001; Gu et al., 2002) or rat cortical neurons (Bassell et al., 1998), have been identified. However, it is not clear so far whether these proteins need the hnRNP RSmn complex for this effect, or whether motoneurons specifically require this complex either in concert or independently from ZBP1 and ZBP2. Many attempts have been made to identify Smn-interacting proteins (for review see Meister et al., 2002; Paushkin et al., 2002), but interaction with ZBP1 and ZBP2 has not been found by such efforts. Further experiments with mouse gene knockout models will need to show the specific requirement of ZBP1, ZBP2, and/or the hnRNP R/QSmn complex for axonal ß-actin mRNA transport in motoneurons and other types of neurons.
It is possible that the role of hnRNP RSmn in motoneurons goes beyond transport of ß-actin mRNA. hnRNP Q has been implicated in regulation of mRNA stability (Grosset et al., 2000), editing (Blanc et al., 2001; Lau et al., 2001), and splicing (Mourelatos et al., 2001). Therefore, the precise function of these RNA-binding proteins in axons remains to be determined. For example, it could be that the interaction of ß-actin mRNA and hnRNP RSmn also controls stability of ß-actin mRNA in motor growth cones. It could also be that this complex is part of a regulatory machinery that controls local actin protein translation in response to extracellular stimuli, which control growth cone migration, presynaptic differentiation, and functions at the motor endplate. This scenario appears as a tempting model to interpret the pathophysiology of SMA. Recently, it has been proposed that the primary function of actin in the growth cone is not propulsion, but is to act as a scaffold for regulatory molecules in the presynapse (Sankaranarayanan et al., 2003). Specific defects in neurofilament distribution in motor axons, as well as defects of axonal sprouting and axonal growth, were observed in another mouse model (Cifuentes-Diaz et al., 2002). Although we did not find a direct effect on the distribution of neurofilaments in axons, a relative accumulation in growth cones was observed. However, this appears as a consequence to reduced ß-actin content in growth cones and reduced size of the axon terminals. In this scenario, proteins that normally are not present in presynaptic structures, such as neurofilaments and microtubules, appear more distal in growth cones. The reduced levels of actin suggest that functions such as growth cone movement and the release of synaptic vesicles (Doussau and Augustine, 2000; Bloom et al., 2003), which also require actin, might be disturbed in SMA, and thus contribute to the specific pathology of this disease. Defects in dynamic processes that are necessary for further maturation and function of the presynaptic part of the motor endplate could thus constitute a major part of the pathophysiology of SMA. Future experiments will have to show whether the hnRNP RSmn complex also modulates synaptic excitability, and thus plays a role that goes beyond the observed effect on axon growth in motoneurons.
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Materials and methods |
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The entire 3' UTR of murine ß-actin cDNA as well as a fragment containing the putative zipcode region (Kislauskis et al., 1994) including a poly(A)+ tail of 30 nucleotides were cloned by RT-PCR into pTZ19, and primers were as follows: actinUTR forward, 5'-ATGAAAGCTTAGGCGGACTGTTACTGAGCTGC-3'; actinUTRpolyAfull reverse, 5'-ATGAGAATTC-T(30)-GTGTAAGGTAAGGTGTGCAC-3'; actinUTRpolyAzip reverse, 5'-ATGAGAATTC-T(30)-CTGCGCAAGTTAGGTTTTGTC-3'; and actin UTRfull reverse, 5'-ATGAGGATCCGTGTAAGGTAAGGTGTGCAC-3'. For RT-PCR of rat ß-actin and c-Jun from PC12 cells, primers were used as follows: Rn actinup, 5'-AAGACCTCTATGCCAACACAG-3'; Rn actinlow, 5'-CTGCTTGCTGATCCACATCTG-3'; RncJunup, 5'-AGCGCATGAGAAACCGCATCG-3'; and RncJunlow, 5'-TGCAACTGCTGCGTTAGCATG-3'. PCR reactions were performed under standard conditions using BioThermTM polymerase (GeneCraft, Ltd.) in the presence of 1 M betaine (Sigma-Aldrich).
PC12 culture and transfections
PC12 cells were routinely maintained in DME plus 10% horse serum, 5% FCS, and antibiotics (Invitrogen). For transfection experiments, the cells were plated at high density in 24-well plates and transfected with 1 µg plasmid DNA (empty vector and/or hnRNP R constructs and/or Smn constructs, to keep the total DNA amount constant) using LipofectAMINETM 2000 (Invitrogen) according to the manufacturer's instructions. The day after transfection, cells were replated in 35-mm dishes containing poly-DL-ornithinecoated glass coverslips at a 1,000-cells/cm2 density in DME plus 2% horse serum, 1% FCS, antibiotics, and 50 ng/ml NGF to stimulate neurite outgrowth. After 3 d of NGF treatment, the cells were fixed and stained as described below.
To establish stable cell lines, transfected PC12 cells were selected for resistance to G418, and individual clones were tested for stable expression by Western blot analysis with anti-HA antibody. To assess neurite length, cells were plated as described above and treated with 50 ng/ml NGF for 7 d. Three independent cell lines for each expression vector were used.
Motoneuron culture
Isolation and culture of embryonic motoneurons and genotyping of the individual embryos were performed as described previously (Wiese et al., 1999, 2001; Monani et al., 2000).
Immunocytochemistry
Motoneurons grown for 5 d on glass coverslips were fixed with PFA and subsequently with acetone. After blocking with 10% BSA, the cells were incubated overnight at 4°C with primary antibodies as follows: rabbit antibodies against 1 µg/ml phospho-tau (1 µg/ml; Sigma-Aldrich) and NF-M (1:500; Abcam), a mouse mAb against MAP-2 antibody (1:1,000; CHEMICON International), actin (1:200; Roche), ß-actin (1:1,000; Abcam), ß-tubulin III (1:200; Research Diagnostics, Inc.), and Smn (1:1,000; Transduction Laboratories). Cells were then washed three times with TBS-T (20 mM Tris-HCl, pH 7.6, 137 mM NaCl, and 0.1% Tween 20) and incubated for 1 h at RT with Cy2- and Cy3-conjugated secondary antibodies (1:200; Dianova). After washing with TBS-T, coverslips were embedded in Mowiol.
PC12 cells were fixed with 4% PFA and blocked with 15% normal goat serum plus 0.3% Triton X-100 in PBS, and were incubated overnight at 4°C with antibodies as follows: anti-neurofilament H (1:400; Sigma-Aldrich), rabbit antibodies against hnRNP R (1:100; Rossoll et al., 2002), monoclonal anti-HA (HA.11, 1:250; Covance), monoclonal anti-FLAG M2 (1:500; Sigma-Aldrich), and monoclonal anti-Smn (1:500; BD Biosciences). Cells were then washed, incubated with secondary antibodies, and embedded as described in the previous paragraph. Immunoreactivity was visualized with a confocal microscope (Leica), with identical settings for pinhole and voltage for any panel of analysis.
In situ hybridization
Cells grown on glass coverslips were fixed with 4% PFA in PBS for 15 min at RT, and were then washed with PBS containing 0.1% active diethyl pyrocarbonate for 10 min at RT. Cells were then permeabilized with 0.3% vol/vol Triton X-100 in PBS for 20 min at RT, and endogenous peroxidase activity was quenched through incubation in 0.3% vol/vol H2O2 in methanol for 40 min at RT. Prehybridization, hybridization, and washes were performed according to the manufacturer's instructions (GeneDetect). Oligonucleotide probes (3' biotinylated) were applied to the coverslips at 200 ng/ml (antisense, 5'-GCCGATCCACACGGAGTACTTGCGCTCAGGAGGAGCAATGATCTTGAT-3'; sense control, 5'-CGGCTAGGTGTGCCTCATGAACGCGAGTCCTCCTCGTTACTAGAACTA-3'; GeneDetect).
Hybridized probe was detected through a tyramide signal amplification system (GenPoint; DakoCytomation) according to the manufacturer's instructions. Finally, coverslips were counterstained with hematoxylin, dehydrated, and mounted with VitroClud (Legenbrick). Images were acquired with a microscope (Axiophot; Leica) equipped with a CCD camera using Axioplan 2 software (Leica). Growth cones were visually scored for the absence or presence of actin mRNA signal. Multiple scoring of the same growth cones was avoided by recording the position of the cells.
Western blot analysis
Western blot analysis was performed as described previously (Rossoll et al., 2002). The primary antibodies were used at a dilution of 1:1,000 each: hnRNP R (Rossoll et al., 2002), Smn (Transduction Laboratories), actin (Roche), and ß-tubulin (CHEMICON International).
Data analysis
For the quantification of neurite length and growth cone area, only motoneurons that allowed a clear distinction between axons and dendrites were scored. Axons were identified as phospho-taupositive processes that are at least two times longer than the phospho-taunegative dendrites. Both the longest axonal branches and the total length of all axonal branches were measured. Cultures obtained from four mutant and control embryos (n = 4) from different litters were scored.
In NGF-treated PC12 cells, axons and dendrites cannot be distinguished. Therefore, all neurites were measured and the average of the three longest processes was calculated. For transient transfection of PC12 cells, at least 30 cells were analyzed per treatment group and four independent experiments were performed. Only transfected cells as shown by staining with the HA antibody (hnRNP R constructs) or FLAG antibody (Smn constructs) were scored. For the stable cell lines, the average length of all neurites was calculated and data were collected from three independent cell lines for each group. Images recorded at the confocal microscope were analyzed using the Scion Image software package (Scion Corporation). Data were analyzed using the t test.
mRNA binding assay
ß-Actin clones were linearized at the 3' end with EcoRI, purified, and 0.2 µg was used for in vitro transcription with T7 RNA polymerase (T7 Transcription Kit; MBI Fermentas) in the presence of [32P]CTP. A 500-bp fragment of the chicken lysozyme gene (included in the kit) and a full-length clone of I
B
cDNA were used as specificity controls. Labeled RNA was purified on G-25 columns (Amersham Biosciences) and radioactive counts were measured for standardization. HEK293 cells were transfected with HA-tagged constructs of hnRNP R, Smn, and c-Jun (LipofectAMINETM 2000; Invitrogen). DNA concentration was kept constant by adding empty vector. 48 h after transfection, cells were lysed in IP buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2 mM EGTA, 2 mM EDTA, 10% glycerol, 1% Triton X-100, 0.05% 2-mercaptoethanol, and protease inhibitors; Roche), and HA-tagged proteins were immunopurified by overnight incubation with HA beads (HA.11; Covance). The immunoprecipitate was washed 5x with IP-buffer and 1x with RNA-binding buffer (RBB; 10 mM Tris, pH 7.5, 1.5 mM MgCl2, 250 mM KCl, 2 mM DTT, and 0.25% Triton X-100). Immunoprecipitates were incubated with labeled RNA probes in RBB for 30 min at RT. The RNA-bound immune complexes were washed three times with RBB and RBB plus 5 mg/ml heparin (Sigma-Aldrich). For quantifications, the pellets were resuspended in 20 µl RBB, spotted on nitrocellulose membranes, and exposed to image plates (BAS 2500; Fuji). Bound radioactivity was quantified as photostimulated luminescence/spot area with the AIDA software package (Raytest). Experiments were repeated at least three times, and typical results are shown. Signal intensity for full-length ß-actin 3' UTR bound to full-length hnRNP R was defined as 100% in each experiment. All values were calculated as relative signal intensities.
For RT-PCR of hnRNP Rassociated ß-actin, HA-tagged wild-type and mutant hnRNP R were immunoprecipitated from differentiated PC12 cell lines with HA antibody. Cells were lysed in RBB containing 100 U/ml RNase inhibitor (Superasin; Ambion) and protease inhibitor (Roche), and were incubated with HA beads (HA.11; Covance) for 30 min at 4°C. Beads were washed 5x with RBB, the pellet was resuspended in 500 µl TRIzol® (Invitrogen), and 100 µg glycogen (Roche) was added. RNA was isolated from immunoprecipitates according to the manufacturer's instructions and was reverse transcribed into cDNA using oligo dT primers (SuperScriptTM first strand synthesis system; Invitrogen). RT-PCR was performed with ß-actinspecific primers with 2535 cycles (15 s at 94°C; 15 s at 57°C; 30 s at 72°C). These primers amplify a DNA fragment of 209 bp. Contamination with rat genomic DNA would yield a larger fragment, including an intron of 123 bp. c-Junspecific primers that were able to amplify a 213-bp fragment from rat cDNA were used as a specificity control.
Online supplemental material
Fig. S1 shows immunolocalization of Smn in PC12 cell lines. The online supplemental material is available at http://www.jcb.org/cgi/content/full/jcb.200304128/DC1.
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Acknowledgments |
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This work was supported by the Schilling Stiftund and by the Deutsche Forschungsgemeinschaft, SFB 581 TP B1 and B4. M. Sendtner is the recipient of support from the Initiative Forschung und Therapie für die SMA. Umrao Monani is a recipient of a Development Grant from the Muscular Dystrophy Association of America.
Submitted: 23 April 2003
Accepted: 1 October 2003
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