Article |
Address correspondence to Alan F. Cowman, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Melbourne 3050, Australia. Tel.: 61-3-9345 2555. Fax: 61-3-9347 0852. E-mail: cowman{at}wehi.edu.au
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Abstract |
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Key Words: malaria; micronemes; erythrocyte; function; substitution
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Introduction |
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The invasion process into host cells involves proteins located in specialized exocytic organelles (micronemes, rhoptries, and granula) that define the electron-dense apical pole of the invasive form of all Apicomplexa (Aikawa et al., 1978; Dubremetz and Schwartzman, 1993; Carruthers and Sibley, 1997). These proteins are targeted to their subcellular location, and this is mediated by either specific amino acid motifs or interaction with an escorter protein (Baldi et al., 2000; Di Cristina et al., 2000; Hoppe et al., 2000; Reiss et al., 2001; Cerede et al., 2002; Meissner et al., 2002; Ngo et al., 2003). The secretion of proteins stored in these organelles is essential for the invasion process. A group of micronemal proteins involved in merozoite and sporozoite invasion are each defined by an adhesive extracellular domain, a transmembrane region, and a cytoplasmic tail (Sultan et al., 1997; Sibley et al., 1998; Adams et al., 2001; Michon et al., 2002). These proteins are differentially expressed during the parasite life cycle (Rogers et al., 1992b; Blair et al., 2002). For example, in Plasmodia, the thrombospondin-related anonymous protein (TRAP)* is not expressed in the asexual life cycle, but is an essential protein for sporozoite invasion (Sultan et al., 1997). TRAP-deficient sporozoites are unable to invade mosquito salivary gland cells or liver cells and do not show any gliding motility. It has been proposed that the cytoplasmic domain of TRAP plays a role in connecting the parasite actinmyosin machinery with the external substrate (Kappe et al., 1999). It is interesting to note that besides TRAP, just one other proteinthe circumsporozoite proteinhas been identified as playing a crucial role in the invasion of hepatocytes by sporozoites (Menard et al., 1997).
In contrast, merozoites, the invasive form of the Plasmodia asexual life cycle, use an array of adhesive transmembrane proteins similar to TRAP (Sim et al., 1994; Reed et al., 2000a; Duraisingh et al., 2003). For instance, members of the erythrocyte binding-like superfamily can provide the merozoite with high affinity binding ligands for a range of receptors on the surface of the erythrocyte (Adams et al., 2001). This multiplicity provides the genetic basis for ligand diversity and different host cell receptor specificity. The relative importance and usage of the ligands for invasion of merozoites is strain dependent (Hadley et al., 1987; Okoyeh et al., 1999; Duraisingh et al., 2003). EBA-175 is the ligand for glycophorin A, the dominant glycoprotein on the surface of the erythrocyte (Sim et al., 1994). The interaction of this ligand with its receptor defines one invasion pathway for merozoites and is dependent on sialic acid moieties on the receptor (Camus and Hadley, 1985). Disruption of this receptorligand interaction by either modifying the surface of the erythrocytes or by gene disruption has shown that the merozoite can use other invasion pathways (Dolan et al., 1990; Reed et al., 2000a; Duraisingh et al., 2003). Loss of function of EBA-175 in both sialic aciddependent and independent P. falciparum strains results in a decrease in invasion of chymotrypsin-treated erythrocytes (Duraisingh et al., 2003). This is due to the inability of these EBA-175 parasites to use the chymotrypsin-resistant receptor glycophorin A. Additionally, in a sialic aciddependent strain, disruption of the EBA-175/glycophorin A pathway manifests itself in a dramatic increase of invasion into neuraminidase-treated erythrocytes. The receptor interaction of EBA-175 is mediated by two adhesive modules (called F1/F2) located in the extracellular domain (Adams et al., 1992); however, nothing is known about the role of the cytoplasmic domain.
In this paper, we address the role of the cytoplasmic domain of EBA-175 in merozoite invasion via the glycophorin Adependent pathway of human erythrocytes. We show that trafficking of EBA-175 is independent of its cytoplasmic domain. Further, we show that the cytoplasmic domain of EBA-175 is essential for a functional EBA-175/glycophorin A pathway and can be substituted by the cytoplasmic domain of TRAP. This suggests that the invasion of sporozoite and merozoite forms of Plasmodia into liver cells and erythrocytes, respectively, use the same cellular machinery.
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Results |
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Nine transfection plasmids based on pHH1 were constructed that would introduce specific alterations by allelic replacement into the 3' end of the EBA-175 gene (Fig. 1 B; Triglia et al., 1998). As a control, we used pHH13'R that would replace the 3' end of the endogenous EBA-175 gene with cDNA coding for the same region. This would express normal EBA-175 protein, and these parasites would serve as a comparison for all transfectants. To determine if the cytoplasmic domain of TRAP could substitute for the same region of EBA-175, we constructed pHH1175TRAP that would replace the 3' end of EBA-175 and insert 114 bps of the TRAP gene. Additionally, we used the plasmid pHH1-230 that would allow deletion of the 3' cysteine-rich region, transmembrane, and cytoplasmic domains. This transfection plasmid has been used previously to produce a parasite line with a truncated EBA-175 that disrupts the function of this protein (Reed et al., 2000a). Importantly, transfection and integration of these plasmids into the endogenous EBA-175 gene by allelic replacement would allow expression of the altered EBA-175 protein under the control of the endogenous promoter (Triglia et al., 1998).
The 10 pHH1-based plasmids were transfected into W2mef parasites, and additionally, the plasmids pHH13'R and pHH1-tail were transfected into the 3D7 parasite line. The transfected parasites were selected for integration via a single crossover recombination event to derive: W2mef3'R, W2mefY1419, W2mefY1464, W2mefYY, W2mefEDD, W2mef
15, W2mefY
15, W2mefTRAP, W2mef
tail, W2mef
230, 3D73'R, and 3D7
tail (Fig. 1, C and D). To confirm that the plasmids had integrated, genomic DNA from the parental line W2mef and 3D7 (as well as the 11 transfectants) was probed with an EBA-175 fragment in Southern hybridization experiments (Fig. 1 D). The structure of the integration events in the transfected parasite lines was confirmed by additional restriction enzyme mapping experiments (Fig. 1, C and D; unpublished data). The EBA-175 gene of each transfected parasite line was further analyzed by PCR and sequencing to confirm that the 3' end had been replaced and that the expected mutations, deletion, or insertion were encoded within the gene.
Expression of mutant EBA-175 in the transfected parasites
To confirm that the EBA-175 protein was expressed, we analyzed parasites in schizont stages of parental lines W2mef and 3D7, as well as the transfectants W2mef3'R, W2mefY1419, W2mefY1464, W2mefYY, W2mefEDD, W2mef15, W2mefY
15, W2mefTRAP, W2mef
tail, W2mef
230, 3D7
tail, and 3D73'R parasites by Western blots with anti-EBA175, anti-EBA175-CT, anti-TRAP-CT, and also anti-HSP70 antibodies as a loading control (Fig. 2 A). Anti-EBA175 antibodies detect the ectodomain of the protein, and all of the parasite lines show expression of the appropriately sized protein. Additionally, in 3D7 and in 3D7-derived transgenic parasites, a strong (
100 kD) cross-reactive protein is detected. The W2mef
tail, 3D7
tail, and W2mef
230 parasites express a protein smaller than that observed for W2mef consistent with expression of the truncated protein. Antibodies specific to the cytoplasmic tail of EBA-175 detect the protein in W2mef, and all of the transfectants except for W2mefTRAP, W2mef
tail, W2mef
230, and 3D7
tail. This shows that these transfected parasite lines express EBA-175 that lacks the cytoplasmic domain. The anti-EBA175-CT antibodies detect EBA-175 in W2mefY
15 and W2mef
15 despite the deletion of 15 amino acids. This is not surprising, as both of these truncated proteins retain 49 amino acids of the 64-amino acid cytoplasmic tail to which the antibody was raised.
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The level of expression of the different mutant and chimeric EBA-175 proteins in each of the transgenic parasites was approximately the same as judged by multiple Western blots using independent schizont preparations (Fig. 2; unpublished data). However, to further investigate the level of transcription of EBA-175 in W2meftail and 3D7
tail parasites, W2mef3'R, 3D73'R, and parasite transcripts of the EBA-175 gene were quantified by real-time RT-PCR and compared with the relevant transfection controls W2mef3'R and 3D73'R (Fig. 2 B). No significant differences between W2mef3'R, W2mef
tail, 3D73'R, and 3D7
tail could be detected.
The cytoplasmic domain of EBA-175 is not required for localization of the protein to the micronemes
Previously, it has been shown that the cytoplasmic tail of type 1 transmembrane proteins in Apicomplexa can contain sorting signals that are essential for correct subcellular localization (for review see Joiner and Roos, 2002). To determine if the mutant EBA-175 proteins were correctly localized to the micronemes, we used immunofluorescence to test colocalization with other microneme proteins including EBA-181 (Gilberger et al., 2003) and EBA-140. Using the parasite line W2mef230, in which EBA-175 is expressed without the 3' cysteine-rich region, transmembrane, and cytoplasmic domain, we showed that this truncation leads to incorrect localization of truncated EBA-175 (Fig. 3). Although still detectable in schizonts (Reed et al., 2000a), it does not colocalize with the microneme marker EBA-181 and importantly is not detectable in merozoites, as it appears to be released into the supernatant on schizont rupture (Fig. 3). This was confirmed by colocalization with a second microneme marker EBA-140 (unpublished data). Previously, it has been suggested that the same truncated EBA-175 may be localized to micronemes in schizont stages; however, no additional microneme markers were available at that time to confirm this result, and immuno-localization in free merozoites was not performed (Kaneko et al., 2000; Reed et al., 2000a).
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To test the role of charged amino acid residues in EBA-175 trafficking, the amino acid residues E-1466, D-1467, and D-1470 were mutated in W2mefEDD using the transfection plasmid pHH1-EDD. Immunofluorescence of EBA-175 in W2mefEDD showed colocalization with EBA-181 in free merozoites, demonstrating that it was located within the micronemes (Fig. 3). To further investigate the possible synergistic role for trafficking of the acidic acid cluster and the tyrosines, we constructed the parasites W2mef15 and W2mefY
15. These parasites express a truncated EBA-175 without the COOH-terminal acidic acid cluster EDD and without either one or both tyrosines. Immunofluorescence experiments with these transgenic parasites showed the same subcellular localization of mutated EBA-175 and EBA-181 in free merozoites, confirming that they are both present in micronemes (Fig. 3).
Finally, to determine if the cytoplasmic tail is required for trafficking of EBA-175 to the micronemes at all, we constructed transfected parasites W2meftail and 3D7
tail that express truncated forms of the protein. Although these parasites express EBA-175 without a cytoplasmic domain, this truncated protein is still trafficked to the micronemes (Fig. 3). Therefore, the cytoplasmic domain of EBA-175 is not required for localization of the protein to micronemes in P. falciparum.
The cytoplasmic domain of EBA-175 is required for function in merozoite invasion
W2mef invades neuraminidase-treated erythrocytes very inefficiently because it is reliant on sialic aciddependent invasion (Dolan et al., 1990), and EBA-175 is an important ligand in this process (Duraisingh et al., 2003). Additionally, the loss of function of EBA-175 can be directly measured in W2mef by a decrease in invasion of chymotrypsin-treated erythrocytes due to the inability of these parasite lines to use the chymotrypsin-resistant erythrocyte receptor glycophorin A (Duraisingh et al., 2003).
The parasite 3D7 can invade via sialic acidindependent receptors on the erythrocyte, and disruption of the EBA-175 gene does not cause a major shift in use of different receptors as has been described for W2mef, suggesting that it is not required for a dominant invasion pathway in this particular parasite line. However, EBA-175 is functional, and this has been demonstrated by a significant decrease in the ability of EBA-175 3D7 parasites to invade chymotrypsin-treated erythrocytes (Duraisingh et al., 2003).
These parameters provided a measure of EBA-175 function in merozoite invasion and allowed us to test the functionality of mutant EBA-175 ligands in transgenic parasites. To determine the effect of the different mutations in the EBA-175 cytoplasmic domain on the function of this protein in merozoite invasion, we first tested the ability of the transgenic parasites to invade neuraminidase-treated erythrocytes (Reed et al., 2000a; Duraisingh et al., 2003). The loss of EBA-175 function in transgenic parasites that resulted in an increased invasion of neuraminidase-treated erythrocytes was confirmed by testing their ability to invade chymotrypsin-treated cells.
W2mefY1419, W2mefY1464, W2mefYY, W2mefEDD, W2mef15, and W2mefY
15 invaded neuraminidase-treated erythrocytes as inefficiently as parental W2mef and the transfection control W2mef3'R, suggesting that EBA-175 was fully functional (Fig. 4 A). This suggested that these specific amino acid residues are not required for the function of EBA-175 in P. falciparum. This was in contrast to the loss of function of EBA-175 as a result of deletion of 54 amino acids from the cytoplasmic tail. W2mef
tail parasites that express a truncated form of EBA-175 lacking the cytoplasmic domain invade neuraminidase-treated erythrocytes efficiently (77%), whereas parental parasites W2mef and the transfection control (W2mef3'R) invade at
16% compared with the invasion of untreated RBCs (Fig. 4 A). The loss of function of the truncated EBA-175 proteins was verified by demonstration that W2mef
tail parasites invade chymotrypsin-treated erythrocytes significantly less than W2mef and W2mef3'R parasites (Fig. 4 B). This switch in invasion phenotype was analogous to that seen for the W2mef
230 parasites (Fig. 4, A and B; Reed et al., 2000a; Duraisingh et al., 2003). The baseline level for reinvasion between mutant EBA-175 parasites and wild-type parental lines is higher compared with that described previously (Duraisingh et al., 2003). This is likely to be a reflection of the altered quantification methods and assay conditions used in the independent studies; however, importantly, the magnitude of the differences is similar in this paper compared with previous data (Reed et al., 2000a; Duraisingh et al., 2003).
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The cytoplasmic domain of EBA-175 can be substituted by TRAP
EBA-175 functions in merozoite invasion of erythrocytes (Sim et al., 1994; Reed et al., 2000a), whereas TRAP is required for invasion of sporozoites into liver cells (Sultan et al., 1997). To test if the cytoplasmic tail of the TRAP protein was able to substitute for the same region in EBA-175, we replaced the COOH-terminal 54 amino acids with the last 37 amino acids of TRAP (Fig. 1 B). As expected, no fluorescence was detectable with anti-TRAP antibodies in W2mef. However, anti-TRAP-CT detected the EBA-175TRAP chimeric protein in W2mefTRAP parasites, and this protein showed colocalization with the micronemal marker EBA-181 in schizonts and merozoites (Fig. 3 and Fig. 5).
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Discussion |
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Specificity of host cell target for sporozoites and merozoites presumably resides in the specific ligand(s) expressed in each life cycle stage. For example, merozoites express the ligands EBA-175 and EBA-140, and these bind glycophorin A (Sim et al., 1994) and C (Maier et al., 2003; Mayer et al., 2001; Thompson et al., 2001). In contrast, the TRAP protein in sporozoites binds to receptors on hepatocytes, probably via heparin sulfate or certain sulfated glycoconjugates (Holt et al., 1990; Cerami et al., 1992). After initial receptor binding of the ligands, they presumably participate in movement of the tight junction between the host cell and merozoite or sporozoite by binding of the cytoplasmic tail either directly or via an adaptor protein to an actinmyosin based motor system (Barnwell and Galinski, 1998; Pinder et al., 1998; Opitz and Soldati, 2002). The ability to substitute the cytoplasmic tail of TRAP for that of EBA-175 suggests that both are able to bind to the same adaptor protein required for merozoite invasion via glycophorin A on the erythrocyte.
Analysis of TRAP cytoplasmic tail function in the mouse malaria Plasmodium berghei (PbTRAP) has shown that specific alterations can block infectivity of hepatic cells and in addition, drastically modify gliding motility (Kappe et al., 1999). It has been suggested that the PbTRAP tail is bifunctional, with the proximal part required for protein translocation along the cortical microfilaments, whereas the distal region contains the signal for release of TRAP at the posterior end, probably by proteolytic cleavage. A penultimate tryptophan residue and a group of acidic amino acid residues present near the COOH terminus of the PbTRAP tail have been shown to be critical for invasion and normal gliding movement (Kappe et al., 1999). The tryptophan residue is conserved in a number of putative TRAP paralogues and orthologues that function in sporozoites and tachyzoites from Apicomplexan parasites; however, it is not present in EBA-175 or any of its paralogues known to be involved in merozoite invasion of erythrocytes (Fig. 1 A). Additionally, the acidic residues at the COOH terminus of EBA-175 are not required for normal function in merozoite invasion, and these results suggest differences in the role of the EBA-175 cytoplasmic tail compared with that suggested for TRAP. The EBA-175 tail may be involved in protein translocation along the cortical microfilaments, probably via an adaptor protein similar to that envisaged for TRAP, but it is likely that release at the posterior pole of the sporozoite or merozoite for the two proteins may involve different processes (Sultan et al., 1997; Kappe et al., 1999). It would be interesting to test the interchangeability of the cytoplasmic domains for sporozoite invasion by generating a parasite line with a chimeric TRAP/EBA175 protein, either with or without an additional penultimate tryptophan.
The ability to substitute the EBA-175 cytoplasmic tail with the equivalent region of TRAP would imply amino acid conservation. However, there is no obvious homology in this region between EBA-175 and TRAP, suggesting that the functional determinants are dependent on the tertiary structure of the cytoplasmic domains. This is consistent with the lack of amino acid homology between the EBA-175 cytoplasmic tail and its paralogues, such as EBA140 and EBA-181 (Fig. 1 A; Gilberger et al., 2003; Maier et al., 2003). The main feature of the cytoplasmic domain of these proteins is their highly charged nature, and it is likely that this is a key element in interaction with a putative adaptor protein and the merozoite invasion machinery. To further analyze sequence requirements for the proposed interaction, it would be of great interest to test if a charged mutant and/or random cytoplasmic domain of approximately the same size could functionally substitute the endogenous EBA-175 domain.
The general importance of the cytoplasmic domain is demonstrated by the deletion of the cytoplasmic domain of EBA-175 in either W2mef or 3D7 (W2meftail and 3D7
tail) parasites that express functionally inactive forms of this ligand. The reported phenotype of these parasite lines was not due to lower expression of the mutant protein, as previously described for certain circumsporozoite protein mutants in P. berghei (Thathy et al., 2002), nor was it a result of incorrect trafficking as the protein was localized to the micronemes (Di Cristina et al., 2000). The EBA-175
tail mutants show a complete loss of EBA-175 function in both sialic aciddependent and independent parasites.
The importance of specific amino acid motifs in the cytoplasmic tail of type 1 transmembrane proteins for post-secretory targeting to their subcellular location has been intensively studied in higher eukaryotes. These proteins enter the secretory pathway with their NH2-terminal signal sequence that cotranslationally direct the proteins into the ER. Type 1 transmembrane proteins can have tyrosine-based motifs in their cytoplasmic tail required for post-Golgi sorting by binding to adaptor protein complexes that are involved in vesicular transport (Bonifacino and Dell'Angelica, 1999). In contrast to this detailed knowledge, very little is known about the molecular mechanisms of protein trafficking in Apicomplexa. It has been shown in T. gondii that tyrosine-based motifs and an acidic amino acid motif are also required for sorting and trafficking of type 1 membrane proteins to the micronemes and rhoptries (Di Cristina et al., 2000; Hoppe et al., 2000; Reiss et al., 2001). This is in contrast to EBA-175 in P. falciparum, a protein that does not require tyrosine-based motifs or indeed the cytoplasmic tail for correct sorting to the micronemes, although this region is essential for the function of this protein in invasion.
The rhoptry protein RAP1 acts as an escorter for RAP2 in P. falciparum, suggesting that complex formation may be important in sorting and subcellular targeting in Apicomplexan parasites (Baldi et al., 2000). Similarly, in T. gondii, the type 1 transmembrane protein MIC6 and MIC8 act as escorters for the soluble proteins MIC1, MIC4, and MIC3 for trafficking to the micronemes, and these complexes appear to play an important role in the invasion of tachyzoites into mammalian cells (Reiss et al., 2001). The cytoplasmic domains of MIC6 and MIC8 appear to contain the information required for correct microneme localization of the complexes, as has been shown for MIC2 (Di Cristina et al., 2000). Deletion of the transmembrane and cytoplasmic domain of MIC8 led to incorrect localization of the protein (Cerede et al., 2002), and this is similar to the results observed with deletion of the 3' cysteine-rich region, transmembrane, and cytoplasmic domain of EBA-175 (Fig. 3). However, it was unexpected that the cytoplasmic domain of EBA-175 was not required for protein sorting to the micronemes. Correct sorting of truncated EBA-175 lacking the cytoplasmic tail to the micronemes suggests another protein with the necessary localization signals may be involved in directing this protein and its paralogues to their subcellular localization.
The results presented here suggest a model for trafficking of EBA-175 that involves an escorter protein specifying the targeting information required for microneme localization (Fig. 6, A and B). We presume that EBA-175 and other microneme proteins are processed through the classical secretory pathway involving the ER and Golgi followed by unknown sorting machinery in the TGN (Fig. 6 A). The trafficking of EBA-175 to the micronemes is independent of the cytoplasmic domain, and we propose that an escorter protein forming a complex is required for this specific sorting event (Fig. 6 B). The inability of EBA-175230 protein to be trafficked to the micronemes suggests that the cysteine-rich region and/or the transmembrane domain are involved in complex formation. It is likely that the 3' cysteine-rich region is important in protein sorting, as it is conserved in other paralogues that function in merozoite invasion, and it is likely that these proteins are sorted and localized to the micronemes by identical mechanisms.
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Materials and methods |
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Nucleic acid and sequence analysis
Chromosome 7 of P. falciparum was sequenced by the Sanger Center (http://www.sanger.ac.uk; Gardner et al., 2002). Preparation of genomic DNA was performed as described previously (Triglia et al., 1998). DNA sequencing was performed using BigDye® Terminator Cycle sequencing (PerkinElmer). Southern blotting was performed using standard procedures. In vitro mutagenesis of the 3' region of EBA-175 was achieved using cDNA in either a one- or two-step primer-directed PCR mutagenesis method with proof reading Vent polymerase (Stratagene). An 1-kb fragment of the 3' end of the EBA-175 gene was amplified with the following primers and sequenced in order to detect unwanted mutations: 175-S, 5'-TGAGGATCCAGGAAATGATACATCTGAAATGTCGCA-3'; 175 3'R-AS, 5'-AGTCTCGAGTCATATCTTAAATTTAATATCATT-3'; 175Y1464-AS, 5'-GCGCCTCGAGTCATATCTTAAATTTAATATCATTTATATCCTCATGCCTTCAG-3'; 175EDD1470-AS, 5'-AGTCT-CGAGTCATATCTTAAATTTAATACCATTTATACCCCCATGGTA-3'; 175
15-AS, 5'-GCGCCTCGAGTCAATCGTTGATATTAGTTT-3'; 175
tail-AS, 5'-AGCCTCGAGTCAACTTTGATATTTGGCTTGTGA-3'; 175Y1419-AS, 5'-CTTTGACCTTTGGCTTGTGAAGC-3'; 175Y1419-S, 5'-GCTTCACAAGCCAAAGGTCAAAG-3'; 175TRAP-AS, 5'-GGCAAAGGGTGTTGCACTTTGATATTTGGCTTGTGAAGC-3'; TRAP-S, 5'-GCAACACCCTTTGCCGGA-3'; TRAP-AS, 5'-AGTCTCGAGTTAATTCCACTCGTTTTCTTC-3'. The bases in italics refer to restriction enzyme sites used for cloning.
All PCR products were subcloned into BamHI/XhoI pBluescript®, amplified, and digested with BamHI and XhoI. Using the compatibility of the 5' overhangs of BglII and BamHI, the fragments were subsequently cloned into a BglII/XhoI precut pHH1 transfection vector (Reed et al., 2000b). To construct pHH1-YY and pHH1Y15, the pBluescript® construct pBSEBA175Y1419 was used as a template for PCR mutagenesis.
Antisera and immunoblots
Rabbit and mouse antisera were raised against the cytoplasmic domain of EBA175 and PfTRAP using the pGEX system (Amersham Biosciences). Other antibodies used in immunodetection were rabbit anti-EBA175 (Reed et al., 2000b) and anti-HSP70 antibodies. For immunoblots, parasite proteins from a synchronized culture were separated on 6% SDS-PAGE gels and transferred to nitrocellulose membranes (Schleicher & Schuell). EBA175-CT, TRAP-CT, and EBA-175 rabbit antisera were diluted 1:250, and HSP70 antisera was diluted 1:5,000 in PBS with 1% wt/vol skim milk. The secondary antibody was sheep antirabbit IgG HRP (Silenus Laboratories). The immunoblots were developed by chemiluminescence using ECL (Amersham Biosciences).
Real-time RT-PCR
Synchronized parasites were harvested in the late schizont stage and total RNA was isolated using TRIzol® (Invitrogen). RNA was further purified by DNase digestion on an RNeasy® column (QIAGEN). 2 µg of total RNA were reverse transcribed either with or without SuperScriptTM II using Anchor T and random nonamers (Invitrogen). The LightCycler® (Roche) was used to quantitate cDNA using QuantiTect SYBR® Green PCR Kit (QIAGEN) and gene specific primers. Reaction conditions were 94°C for 15 min, 45°C for 20 min, and 65°C for 30 min. EBA175F, 5'-AATTTCTGTAAAATATTGTGACCATAT-3'; EBA175R, 5'-GATACTGCACAACACAGATTTCTT-3'; ACTINF, 5'-TGCACCACCAGAGAGAAAAT-3'; ACTINR, 5'-ACTTGGTCCTGATTCATCGT-3'; H2BF, 5'-TGGTCCAGATGGAAAGAAAA-3'; and H2BR, 5'-TCGAAAGTATCAACAAGGAATG-3'.
Serial dilutions of 3D7 genomic DNA were used as a standard reference control. The relative expression ratios of EBA175 compared with the two reference genes, actin and histone 2B, were calculated for each strain after subtracting the signal generated by samples with no reverse transcriptase.
Immunofluorescence
Immunofluorescence assays were performed with synchronized parasites. Late schizonts were smeared, air dried, and fixed for 1 min with 100% methanol at -20°C. Slides were incubated for 1 h with a mixture of rabbit anti-EBA175 (1:1,000) or anti-TRAP-CT (1:1,000) and mouse anti-EBA181 (1:1,250), washed three times for 10 min with 0.05% Tween 20-PBS, and then incubated for 1 h with fluorescein isothiocyanatelabeled sheep antimouse IgG antibodies (Silenus Laboratories), rhodamine-labeled goat antirabbit IgG antibodies (CHEMICON International) and DAPI (1:1,250; Boehringer). Dual-color fluorescence images were captured using a microscope (Axioskop 2; Carl Zeiss MicroImaging, Inc.) and a digital camera (PCO sensicam).
Erythrocyte enzyme treatments and invasion assay
Parasite erythrocyte invasion assays were performed to characterize the invasion phenotype of the W2mef-EBA175 transgenic lines (Reed et al., 2000a). Surface modification of the erythrocytes was achieved by similar treatment as described elsewhere (Dolan et al., 1994): 500 µl 1 µg/ml chymotrypsin of packed erythrocytes were incubated with either 50 mU neuraminidase or 1.5 mg/ml trypsin. Tightly synchronized parasite cultures were treated with neuraminidase and trypsin to prevent reinvasion. Ring stage cultures were adjusted to 2% parasitemia and treated with neuraminidase for 1 h at 37°C (50 mU for 200 µl packed, infected erythrocytes in 1 ml RPMI 1640/Hepes/0.2% NaHCO3). After washing twice with RPMI 1640/Hepes/0.2%NaHCO3, the infected cells were incubated with trypsin (1 mg/ml in RPMI 1640/Hepes/0.2% NaHCO3) for 1 h at 37°C followed by incubation with trypsin inhibitor (1 mg/ml in RPMI 1640/Hepes/0.2% NaHCO3; Sigma-Aldrich) for 10 min at RT. The cultures were washed, resuspended in complete medium, and incubated overnight under standard conditions. The invasion assays were set up the next day by mixing equal amounts of infected double-enzyme treated cells (schizont stage) with uninfected normal or enzyme-modified erythrocytes to a final hematocrit of 3%. 100-µl aliquots of each culture in triplicate were put into a 96-well microtiter plate. The parasitemia in each well was counted by FACScanTM using Retic-COUNTTM (Becton Dickinson). After 36 h of incubation, the parasitemia of each well was counted again by FACScanTM. Parasitemias were confirmed by microscopic counting of Giemsa-stained thin blood smears and subsequently compared with the starting parasitemia. Control wells with unstained culture and stained uninfected erythrocytes were included in FACScanTM and in the microscopic counting to enable background correction.
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Acknowledgments |
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This work was supported by the National Health and Medical Research Council of Australia and the Wellcome Trust. T.-W. Gilberger is funded by the Deutsche Forschungsgemeinschaft through a Emmy Noether Fellowship. A.F. Cowman is a Howard Hughes International Scholar.
Submitted: 14 January 2003
Revised: 5 June 2003
Accepted: 9 June 2003
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