Protein Tyrosine Phosphatase {alpha} (PTP{alpha}) and Contactin Form a Novel Neuronal Receptor Complex Linked to the Intracellular Tyrosine Kinase fyn

Li Zenga, Luca D'Alessandrib, Markus B. Kalouseka, Lloyd Vaughanb, and Catherine J. Pallena
a Institute for Molecular and Cell Biology, Singapore 117609, Republic of Singapore
b Institute for Biochemistry, ETH-Zurich, CH-8092 Zurich, Switzerland

Correspondence to: Catherine J. Pallen, Institute for Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, Republic of Singapore. Tel:65-874-3742 Fax:65-779-1117


  Abstract
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Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
References

Glycosyl phosphatidylinositol (GPI)–linked receptors and receptor protein tyrosine phosphatases (RPTPs), both play key roles in nervous system development, although the molecular mechanisms are largely unknown. Despite lacking a transmembrane domain, GPI receptors can recruit intracellular src family tyrosine kinases to receptor complexes. Few ligands for the extracellular regions of RPTPs are known, relegating most to the status of orphan receptors. We demonstrate that PTP{alpha}, an RPTP that dephosphorylates and activates src family kinases, forms a novel membrane-spanning complex with the neuronal GPI-anchored receptor contactin. PTP{alpha} and contactin associate in a lateral (cis) complex mediated through the extracellular region of PTP{alpha}. This complex is stable to isolation from brain lysates or transfected cells through immunoprecipitation and to antibody-induced coclustering of PTP{alpha} and contactin within cells. This is the first demonstration of a receptor PTP in a cis configuration with another cell surface receptor, suggesting an additional mode for regulation of a PTP. The transmembrane and catalytic nature of PTP{alpha} indicate that it likely forms the transducing element of the complex, and we postulate that the role of contactin is to assemble a phosphorylation-competent system at the cell surface, conferring a dynamic signal transduction capability to the recognition element.

Key Words: PTP{alpha}, tyrosine phosphatase, glycosyl phosphatidylinositol, neural signal transduction


  Introduction
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Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
References

THE neural cell adhesion molecule contactin (F3/F11) contains Ig-like domains and FN-III repeats, and is attached to the cell surface through a glycosyl phosphatidylinositol (GPI)1 anchor (Ranscht and Dours 1988 ; Brummendorf et al. 1989 ; Gennarini et al. 1989 ; Zisch et al. 1992 ). This mode of membrane attachment precludes direct transfer of signals into the cell, but allows high mobility in the plane of the membrane, and as such ideally suits contactin for responding to macromolecular stimuli presented to the cell (Vaughan 1996 ). The modulation of signal transduction would critically depend on cis association with transmembrane components. Lateral cis interactions occur with the Ig superfamily transmembrane receptors Ng-CAM and Nr-CAM (Brummendorf et al. 1993 ; Morales et al. 1993 ; Sakurai et al. 1997 ) and with Caspr (Peles et al. 1997 ). Intriguingly, contactin can be isolated in a complex with the intracellular src family tyrosine kinase fyn (Zisch et al. 1995 ). The mechanism of this association is unknown, but likely requires an intermediate membrane-spanning component.

As many signal transduction pathways are initiated through activation of intrinsic or associated receptor tyrosine kinase activity, the association of fyn with contactin may be central to contactin signaling. Several protein tyrosine phosphatases (PTPs) can activate src family kinases in various systems, suggesting a PTP to be a candidate component of a contactin signaling complex.

The transmembrane nature of the receptor-like PTPs (RPTPs) indicates that they transduce extracellular signals, although few such signals/ligands are known. The identification of extracellular ligands for RPTPs could provide critical insights into the specific functions and regulation of these enzymes. PTP{alpha} is one such RPTP for which a ligand is sought. The short glycosylated extracellular region of PTP{alpha}, unlike many other RPTPs, lacks known adhesion motifs. It is linked via the transmembrane segment to the intracellular region that contains two homologous catalytic domains, both of which have intrinsic phosphatase activity (Wang and Pallen 1991 ). Several properties of PTP{alpha} indicate that it may, among other things, be involved in neuronal signaling. PTP{alpha} is particularly abundant in the brain and implicated in mediating neuronal differentiation, cell proliferation, and transformation, by its ability to activate the tyrosine kinase src (Zheng et al. 1992 ; den Hertog et al. 1993 ). PTP{alpha} also associates with, dephosphorylates, and activates brain fyn (Bhandari et al. 1998 ), a src family kinase that plays a role in axonal growth, myelination, spatial learning and memory (Lowell and Soriano 1996 ). Mice lacking PTP{alpha} have reduced activities of brain src and fyn, demonstrating that PTP{alpha} is a positive physiological regulator of these kinases (Ponniah et al. 1999 ; Su et al. 1999 ).

The shared and complementary features of PTP{alpha} and contactin suggested that they may form a signaling complex. Both proteins are expressed in some of the same neuronal cell types such as migrating cerebellar granule cell neurons (Faivre-Sarrailh et al. 1992 ; Fang et al. 1996 ). At a molecular level, the physical association of PTP{alpha} and fyn raises the possibility that PTP{alpha} provides a transmembrane link between contactin and fyn. Furthermore, the ability of PTP{alpha} to activate fyn indicates that PTP{alpha} could thereby transduce a signal originating from the extracellular engagement of contactin. We demonstrate that PTP{alpha} and contactin associate and define certain requirements for interaction. These findings indicate that contactin is a novel extracellular partner of PTP{alpha} and that this complex may regulate aspects of neuronal development.


  Materials and Methods
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Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
References


Expression Plasmids
Numbering of the human PTP{alpha} amino acid sequence is according to Krueger et al. 1990 . The pXJ41 vectors expressing PTP{alpha}, VSVG-tagged PTP{alpha}, fyn, and CD45 have been described (Zheng et al. 1992 ; Bhandari et al. 1998 ). To construct pXJ41-contactin-neo, two contactin cDNA fragments comprising nucleotides 38–1103 and 177–3961 were obtained from pSP72-contactin/F11 (Zisch et al. 1995 ) and subcloned in two steps into pXJ41-neo to reconstitute the contactin coding sequence. To construct pXJ41-myr-PTP{alpha}-neo, the PCR-amplified region of human src cDNA encoding the NH2-terminal myristylated sequence MGSNKSKPKDASQ was cloned into pGEM-T (Promega Corp.), and the reverse orientation was selected with the multiple cloning NotI site at the 5' end of the myristylation signal. A NotI-XhoI fragment was excised and cloned into pXJ41-neo, creating pXJ41-myr-neo. A PCR fragment of PTP{alpha} encoding the entire intracellular region (amino acids 148–774), and flanked with engineered SalI sites, was inserted in-frame into XhoI-cut pXJ41-myr-neo. To construct pXJ41-PTP{alpha}/CD45-neo encoding the chimeric RPTP, a region of the VSVG–PTP{alpha} cDNA encoding the signal peptide and amino acids 1–121 of PTP{alpha} was amplified by PCR using forward and reverse primers with engineered NotI and BglII sites, respectively. This NotI-BglII fragment was inserted into a pXJ41-neo intermediate vector. A CD45 cDNA fragment encoding 35 extracellular juxtamembrane amino acids, the transmembrane, and the entire intracellular regions was released from pXJ41-CD45-Hy with BglII and inserted into the intermediate vector.


Cell Culture and Transient Transfections
COS-1 cells were maintained and transiently transfected as described (Bhandari et al. 1998 ). The empty expression plasmid pXJ41-neo was used to normalize the amount of DNA in each transfection. After 24 h of coculture, the cells were harvested and processed as described below. For tunicamycin treatment, 20 µg/ml tunicamycin (Boehringer Mannheim) in DME/10% FCS was added to the cells 6 h after transfection, and the cells were harvested after a further 42 h of culture.

COS-7 cells cultured on 1 ml polylysine or alcian blue–coated glass 12-mm-diam coverslips were processed for immunocytochemistry 24–48 h after transfection. Images were taken using a Zeiss Axiovert 100M equipped with a Hamamatsu C5810 3 color CCD cooled camera. Images were processed directly with Adobe Photoshop.


Antibody-mediated Copatching of Contactin and PTP{alpha} in Transfected Cells
Copatching of contactin or VSVG-PTP{alpha} was performed on COS-7 cells 24–48 h after transfection as described (Zisch et al. 1995 ). Cells were incubated on ice with 10 µg anticontactin (4D1) or anti–VSVG antibodies. Bound antibodies were clustered by incubation with a 1:100 dilution of FITC- or RITC-labeled goat anti–mouse antibodies (Tago), followed by fixation with 4% paraformaldehyde and permeabilization (when appropriate) with 0.2% Triton X-100. Affinity-purified rabbit antibodies to contactin or PTP{alpha} (see below) were used complementary to the mAbs, together with goat anti–rabbit antibodies labeled with FITC or RITC (Tago).


Western Blots and Immunoprecipitation
Six embryonic chick brains (15-d-old) were mechanically suspended in 30 ml of ice-cold PBS buffer, filtered through a nylon mesh, homogenized by 10 strokes in a glass Dounce homogenizer, and washed three times in PBS. Chick brain cells or transfected COS cells were lysed in 10 mM Tris-Cl, pH 8, 150 mM NaCl, 1 mM EDTA, 1% Brij-96, 20 µg/ml aprotinin, and 2 mM PMSF, and the lysates were clarified by centrifugation. Antibodies toward VSVG (Sigma Chemical Co.), contactin (4D1) (Zisch et al. 1995 ), CD45 or fyn (Transduction Laboratories), PTP{alpha} (antiserum 2205, raised against PTP{alpha}-D1), and NCAM (Chemicon International, Inc.) were used for immunoprecipitation and/or immunoblotting.


Phosphatase Assays
The phosphatase activity of 5 µl immunoprecipitate toward 2 µM phosphotyrosyl-RR-src peptide was measured at 30°C for 15 min as described (Lim et al. 1997 ).


  Results
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
References


PTP{alpha} and Contactin Are Associated in Chick Brain
Contactin and PTP{alpha} immunoprecipitates from embryonic chick brain lysates were probed for the presence of contactin and PTP{alpha}. PTP{alpha} was present in anticontactin immunoprecipitates (Figure 1 A), and contactin was present in PTP{alpha} immunoprecipitates, but was not precipitated by preimmune serum (Figure 1 B). Thus, PTP{alpha} and contactin exist in a complex in brain lysates. To check the specificity of the association of PTP{alpha} and contactin, we examined the interaction of PTP{alpha} with NCAM, another fyn-associated (Beggs et al. 1997 ) cell adhesion molecule highly expressed in the brain. The 120- and 140-kD NCAM isoforms were detected in anti-NCAM immunoprecipitates from mouse brains, but PTP{alpha} was not present (Figure 1 C). Likewise, NCAM could not be detected in anti-PTP{alpha} immunoprecipitates prepared from these lysates (Figure 1 D) using the same anti-PTP{alpha} antiserum as employed with the chick brain lysates (raised to the species conserved intracellular D1 region of PTP{alpha}).



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Figure 1. PTP{alpha} associates with contactin in chick brain. Embryonic chick (A and B) or adult mouse (C and D) brain lysates (WCL) and immunoprecipitates made with anticontactin antibody (A), anti–NCAM antibody (C), or affinity-purified anti–PTP{alpha} antibody or preimmune (PI) serum (B and D), were probed for the presence of contactin, NCAM, or PTP{alpha} as indicated.


Association of Ectopically Expressed PTP{alpha} and Contactin
To investigate the molecular basis of the association between PTP{alpha} and contactin, we used a transient expression system where the interaction of different forms could be manipulated. PTP{alpha} and contactin coimmunoprecipitated with one another from COS cells coexpressing PTP{alpha} (tagged in its extracellular region with an epitope of VSVG to facilitate immunoprecipitation [ Bhandari et al. 1998 ]) and contactin (Figure 2 A). In other experiments, the immunocomplexes were assayed for PTP activity (Figure 2 B). Anti-VSVG immunoprecipitates from cells expressing PTP{alpha} alone or with contactin contained comparable levels of phosphatase activity, whereas virtually no activity was detectable in anti-VSVG immunoprecipitates from cells expressing contactin alone. Anticontactin immunoprecipitates from coexpressing cells contained about a fivefold higher phosphatase activity than those from cells expressing contactin or PTP{alpha} alone. These results indicate that the contactin–PTP{alpha} complexes are functionally active. The levels of PTP{alpha} protein and phosphatase activity were much lower in anticontactin immunoprecipitates than in anti-VSVG immunoprecipitates from the coexpressing cells, likely because only a portion of the expressed PTP{alpha} associates with contactin.



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Figure 2. Association of ectopically expressed PTP{alpha} and contactin. COS-1 cells were transfected with VSVG-PTP{alpha} ({alpha}), contactin (cont), or VSVG-PTP{alpha} and contactin ({alpha}/cont) cDNAs. (A) Whole cell lysates (WCL), and anti-VSVG or anticontactin immunoprecipitates (IP) were probed with anti-VSVG or anticontactin antibodies. (B) Anti-VSVG and anticontactin immunoprecipitates were assayed for phosphatase activity as described in Materials and Methods. The bars represent the values of the mean phosphatase activity ± SEM measured in three independent experiments. (C) COS-1 cells were transfected with CD45, contactin (cont), or CD45 and contactin (CD45/cont) cDNAs. Whole cell lysates (WCL), and anti-CD45 or anticontactin immunoprecipitates (IP) were probed with anti-CD45 or anticontactin antibodies.

Similar experiments were carried out with contactin and CD45, a receptor-like PTP with structural similarity to PTP{alpha}. No coimmunoprecipitation of contactin and CD45 from coexpressing cells was detected (Figure 2 C), indicating that the interaction of PTP{alpha} and contactin is specific and not merely due to heterologous expression.


Colocalization and Coclustering of Contactin and PTP{alpha}
In situ localization of contactin and PTP{alpha} in cotransfected COS cells revealed a similar distribution for both proteins within the plane of the plasma membrane (Figure 3A and Figure B). In contrast, control transfections of contactin together with the RPTP CD45, gave a completely different pattern of distribution to that of contactin (data not shown), indicating that they do not associate in the same cellular complexes.



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Figure 3. Ectopically expressed PTP{alpha} and contactin colocalize. (A and B) COS-7 cells cotransfected with contactin and VSVG-tagged PTP{alpha} cDNAs were fixed and incubated with a mouse anti–VSVG antibody and with a rabbit anticontactin antibody, with detection by FITC-labeled goat-anti–rabbit (A) and RITC-labeled goat anti–mouse antibodies (B). (C–F) In COS-7 cells cotransfected with contactin and PTP{alpha}, contactin was induced to form cell surface clusters by incubation with mouse 4D1 antibody (C and D) and PTP{alpha} was induced to form cell surface clusters by incubation with mouse anti-VSVG antibody (E and F), in each case followed by FITC-labeled goat anti–mouse antibodies. In the PTP{alpha}-induced clustering, contactin was detected with rabbit anticontactin, and in the 4D1-induced clustering, PTP{alpha} was detected with rabbit anti-PTP{alpha}, followed in both cases by RITC-labeled goat anti–rabbit antibodies. C and F show the distribution of contactin and D and E that of PTP{alpha}, with focus levels close to the plane of cell-substratum attachment and with corresponding structures in each panel marked (asterisk).

We examined whether an enforced redistribution of either contactin or PTP{alpha}, induced by incubating the live cells with antibodies to the extracellular domains of these molecules, would lead to coclustering of the respective partner. Clustering of PTP{alpha} (Figure 3 E) caused contactin to redistribute to closely match the PTP{alpha} pattern (Figure 3 F). The close similarity of these two patterns supports the efficacy of clustering via the free-standing VSVG tag on the NH2-terminal of PTP{alpha}, leading to little or no interference with the interactions between PTP{alpha} and contactin. Coclusters of contactin and PTP{alpha} could also be induced by 4D1 mAb specific for contactin (Figure 3C and Figure D). Clusters of various sizes were induced in individual cells, with the PTP{alpha} localization largely matching the contactin pattern.


The Extracellular Region of PTP{alpha} Is Required for Association with Contactin
To identify the region of PTP{alpha} involved in the interaction with contactin, we generated a membrane-associated intracellular form of PTP{alpha} by replacing its extracellular and transmembrane regions with the myristylation signal of src (myr-PTP{alpha}). Expressed myr-PTP{alpha} was associated with the membrane fraction (data not shown), however, contactin only associated with wild-type PTP{alpha} and not with myr-PTP{alpha} (Figure 4 A). Thus, contactin does not interact with the PTP{alpha} lacking the extracellular and transmembrane regions.



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Figure 4. The extracellular region of PTP{alpha} is required for association with contactin. (A) Lysates (WCL) and anti-PTP{alpha} immunoprecipitates (IP) from COS-1 cells transiently expressing contactin (cont), myr-PTP{alpha}/contactin (myr-{alpha}/cont), or PTP{alpha}/contactin ({alpha}/cont) were probed for PTP{alpha} and contactin. (B) Whole cell lysates (WCL) and anti-VSVG immunoprecipitates (IP) of COS cells transiently expressing contactin (cont), VSVG-PTP{alpha}-CD45/contactin ({alpha}-CD45/cont), or VSVG-PTP{alpha}/contactin ({alpha}/cont) were probed with anti-VSVG or anticontactin antibodies. (C) COS-1 cells were cotransfected with VSVG-PTP{alpha} and contactin cDNAs and treated with 20 µg/ml tunicamycin (lanes 2, 4, 6, and 8) or left untreated (lanes 1, 3, 5, and 7). Whole cell lysates (WCL) and anti-VSVG immunoprecipitates (IP) were probed with anti-VSVG or anticontactin antibodies.

Since CD45 does not associate with contactin, we created a PTP{alpha}/CD45 hybrid molecule where most of the extracellular region of CD45 was replaced with that of PTP{alpha}. PTP{alpha} or the PTP{alpha}-CD45 hybrid was coexpressed with contactin. Anti-VSVG–immunoprecipitated PTP{alpha}-CD45 hybrid and PTP{alpha} were complexed with contactin (Figure 4 B), demonstrating that contactin associates with the extracellular region of PTP{alpha}. Furthermore, the transmembrane region of PTP{alpha} is not specifically involved in the association with contactin since it can be replaced with that of CD45.


N-linked Glycosylation Is Not Required for the Association of PTP{alpha} and Contactin
The mature PTP{alpha} protein contains both N- and O-linked oligosaccharides (Daum et al. 1994 ). Chick contactin has nine potential sites for N-linked glycosylation (Ranscht and Dours 1988 ; Brummendorf et al. 1989 ). When COS cells coexpressing PTP{alpha} and contactin were cultured with tunicamycin, an inhibitor of N-linked glycosylation, faster migrating, less diffuse forms of PTP{alpha} and contactin were detected on SDS-PAGE (Figure 4 C), which is consistent with a loss of N-linked oligosaccharides. Nevertheless, contactin was present in anti-VSVG immunoprecipitates from cells treated with or without tunicamycin (Figure 4 C, lanes 7 and 8), indicating that the association of contactin and PTP{alpha} occurs independently of N-linked glycosylation of either protein.


PTP{alpha} and Contactin Associate In Cis but Not In Trans
Two experiments were carried out to address the question of whether PTP{alpha} and contactin associate in a cis or trans conformation. First, anticontactin precipitates were prepared from lysates of COS cells expressing either PTP{alpha} or contactin, or from cells coexpressing both PTP{alpha} and contactin (Figure 5 A, lanes 1–3), as well as from another sample made by mixing lysates from the cells expressing either contactin or PTP{alpha} (Figure 5 A, lane 4). Anticontactin immunoprecipitates prepared from coexpressing cells contained PTP{alpha}, but those from mixed cell lysates did not (Figure 5 A, bottom, lanes 7 and 8). The lack of detectable association of PTP{alpha} and contactin in the mixed lysates suggests that interaction cannot take place in a trans conformation. Still, this may require a particular presentation of these cell surface molecules in growing cells that cannot form in solubilized cell lysates. Therefore, contactin-expressing cells were trypsinized 24 h after transfection and replated in dishes containing PTP{alpha}-expressing cells (these were not trypsinized for replating because this resulted in a large decrease in PTP{alpha} expression). After 24 h of coculture, the cells were lysed and immunoprecipitates were prepared. As a positive control for contactin–PTP{alpha} association, PTP{alpha}- and contactin-cotransfected cells were cultured for 48 h, harvested, and processed the same way. PTP{alpha} and contactin coimmunoprecipitated from cotransfected cells (Figure 5 B, top, lanes 3 and 5), but not from cocultured cells (Figure 5 B, top, lanes 4 and 6). Thus, even when cells expressing contactin are cultured together with other cells expressing PTP{alpha}, no association in trans of these two receptor proteins occurs.



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Figure 5. PTP{alpha} and contactin do not associate in a trans configuration. (A) COS cells were transfected with VSVG-PTP{alpha} ({alpha}), contactin (cont), or VSVG-PTP{alpha} and contactin cDNAs ({alpha}/cont). Whole cell lysates (WCL) (lanes 1–3), a mixed lysate ({alpha}+cont) (lane 4) made of equal parts of the VSVG-PTP{alpha}–expressing cell lysate, the contactin-expressing cell lysate, and corresponding anticontactin immunoprecipitates (IP) (lanes 5–8) were probed with anticontactin (top) and anti-VSVG (bottom) antibodies. (B) Cells coexpressing VSVG-PTP{alpha} and contactin ({alpha}/cont) were cultured for 48 h and harvested. Contactin-expressing cells were trypsinized 24 h after transfection, replated onto VSVG-PTP{alpha}–expressing cells ({alpha}+cont), and harvested after coculture for 24 h. Whole cell lysates (WCL) (lanes 1 and 2), anti-VSVG (lanes 3 and 4) and anticontactin (lanes 5 and 6) immunoprecipitates (IP) were probed with anticontactin (top) or anti-VSVG (bottom) antibodies.


  Discussion
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Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
References

We demonstrate that a receptor protein tyrosine phosphatase forms a membrane-spanning complex with a neuronal GPI-anchored receptor. PTP{alpha} and contactin associate with one another in a lateral (cis) forming complex mediated through the extracellular region of PTP{alpha}. This complex is sufficiently stable to permit its isolation from brain lysates or transfected cells through immunoprecipitation of either component, and to permit coclustering of PTP{alpha} with contactin upon antibody-induced clustering of contactin within cells and vice versa. Our findings that contactin–PTP{alpha} complexes form in cotransfected cells, but not upon coculture or in mixed lysates of PTP{alpha}-expressing cells and contactin-expressing cells, provides compelling evidence that PTP{alpha} and contactin can only associate within the same cell; thus, forming a receptor complex rather than a ligand–receptor pair.

This is the first identification of an extracellular partner and potential regulator of PTP{alpha}. As contactin lacks any intracellular region and is tethered to the external face of the plasma membrane through a GPI linkage, PTP{alpha} could, thus, link an extracellular contactin-mediated signal to an intracellular response. Fyn is complexed with contactin and is transiently activated upon aggregation of contactin (Zisch et al. 1995 ). Likewise, fyn is associated with PTP{alpha} and is dephosphorylated and activated by PTP{alpha} (Bhandari et al. 1998 ; Harder et al. 1998 ; Ponniah et al. 1999 ; Su et al. 1999 ). The association of PTP{alpha} and contactin suggests that PTP{alpha} might act as an intermediary molecule in a tripartite complex of contactin, PTP{alpha}, and fyn, in accord with its proposed role as a transducer. Zisch et al. 1995 reported that antibody-mediated cross-linking of contactin results in the activation of associated fyn, raising the possibility that cross-linking of contactin–PTP{alpha} and contactin–fyn complexes brings PTP{alpha} into proximity with fyn, allowing the subsequent dephosphorylation and activation of fyn. In view of the constitutive activity of PTP{alpha} (and other RPTPs), such a contactin-regulated access of PTP{alpha} to its substrate provides an attractive mechanism for mediating RPTP activity and the action of GPI-anchored receptors as modulators in larger signaling complexes.

Contactin interacts with multiple ligands and the identification of PTP{alpha} as a novel contactin-associated protein extends the number and the nature of possible contactin-containing receptor complexes. Contactin is found in regions of active neuronal migration or outgrowth in the developing brain and in areas of synaptic development and activity (Faivre-Sarrailh et al. 1992 ; D'Alessandri et al. 1995 ; Zisch et al. 1995 ). The role of this mobile GPI-anchored receptor may well be to deliver the components of a phosphorylation-competent machinery to receptor complexes mediating neuronal motility or synaptic activity. Evidence for such a role has been obtained for the closely related GPI receptor axonin-1/TAG-1, where neurite fasciculation mediated by complexes of axonin-1 and NgCAM is accompanied by a rapid downregulation of fyn phosphorylation (Kunz et al. 1996 ).

An intriguing possibility arising from this study is that contactin acts as an adapter to bring together two RPTPs, namely RPTP{zeta} and PTP{alpha}. The NH2-terminal carbonic anhydrase-like region of the transmembrane and secreted extracellular forms of glial cell RPTP{zeta}/ß associates in trans with contactin, and in doing so promotes neurite growth (Peles et al. 1995 ). If PTP{alpha} functions as a signaling component of a RPTP{zeta}/ß–bound contactin–PTP{alpha} complex, this would represent a novel mode of RPTP interaction and regulation.

The proposed signaling through a contactin–PTP{alpha} complex represents a new paradigm of receptor-mediated tyrosine kinase activation. Receptors with intrinsic tyrosine kinase activity or directly associated with active nonreceptor tyrosine kinases have been well documented. Contactin, lacking the intracellular region required for either of these mechanisms, may utilize an associated RPTP, PTP{alpha}, to effect intracellular activation of tyrosine kinases. This is reminiscent of the recent finding that the GPI-anchored cell surface receptors GDNFR-{alpha} and NTNR-{alpha} form functional coreceptor complexes with the transmembrane tyrosine kinase Ret (Buj-Bello et al. 1997 ; Klein et al. 1997 ) and underlines the concept that GPI-anchored receptor signaling is achieved by modulation of protein tyrosine phosphorylation. Our study unites previous progress in two areas of research: the interaction of extracellular ligands with the neural cell adhesion molecule contactin, and the intracellular signaling events mediated by PTP{alpha}. The components of a novel signal transduction pathway, thus, have been identified and can now be tested for function and physiological relevance to aspects of neuronal development.


  Footnotes

L. Zeng, L. D'Alessandri, and M.B. Kalousek contributed equally to this work.
M.B. Kalousek's present address is Laves-Arzneimittel GmbH, CH-6247 Schötz, Switzerland.
1 Abbreviations used in this paper: FN-III, fibronectin type III; GPI, glycosyl phosphatidylinositol; PTP, protein tyrosine phosphatase; RPTP, receptor protein tyrosine phosphatase; VSVG, vaccinia stomatitis virus glycoprotein.


  Acknowledgements
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Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
References

We thank G. Koretzky for the CD45 cDNA, P. Bello for the pp60c-src cDNA, V. Bhandari, J.E. Walker, and K.L. Lim for construction of pXJ41-PTP{alpha}/CD45-neo, pXJ41-contactin-neo, and pXJ41-myr-PTP{alpha}-neo, and C. Cagnoli for help with preparation of anti–PTP{alpha} antibodies.

This work was supported by the Krebsforschung Schweiz and the Swiss National Science Foundation (to L. Vaughan) and the National Science and Technology Board of Singapore (to C.J. Pallen).

Submitted: 14 September 1999
Revised: 1 October 1999
Accepted: 4 October 1999


  References
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Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
References

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