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Address correspondence to Suzanne R. Pfeffer, Dept. of Biochemistry, B400, Stanford University School of Medicine, Stanford, CA 94305-5307. Tel.: (650) 723-6169. Fax: (650) 723-6783. E-mail: pfeffer{at}cmgm.stanford.edu
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Abstract |
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Key Words: endosome; Rab9; Golgi complex; Rab7; TIP47
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Introduction |
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TIP47 binds with high specificity to the cytosolic domains of the cation-dependent and cation-independent MPRs (Diaz and Pfeffer, 1998). Indeed, TIP47 fails to bind to other proteins that are transported from late endosomes to the Golgi complex including furin, carboxypeptidase D, TGN38, and the HIV Nef protein (Krise et al., 2000). TIP47 recognizes a Phe-Trp motif in the CD-MPR (Diaz and Pfeffer, 1998) and a proline-rich region of the CI-MPR (Orsel et al., 2000). We have shown recently that TIP47 also binds directly to Rab9 (Carroll et al., 2001). TIP47 can bind to Rab9 and MPRs simultaneously via physically distinct domains. Moreover, Rab9 increases the affinity of TIP47 for MPR cytoplasmic domains (Carroll et al., 2001). In this way, it appears that Rab9 facilitates the process of MPR cargo collection during the process of transport vesicle formation.
But what are the transport carriers that deliver MPRs from late endosomes to the Golgi complex? Function blocking anticlathrin antibodies failed to inhibit MPR recycling to the trans-Golgi under conditions in which they strongly interfered with transferrin endocytosis (Draper et al., 1990). Thus, it appears that clathrin may not play a role in MPR recycling. TIP47 may replace clathrin in this step because it occurs as a large oligomer in cytosol, and it may have the capacity to bind cargo and to function as a coat protein.
We report here experiments designed to visualize MPR trafficking in live cells. Although to date we have been unable to generate a functional fluorescent TIP47 protein, we succeeded in monitoring green fluorescent protein (GFP) variants of Rab9 by video microscopy. We describe here the morphology and motility of organelles and transport vesicles that carry Rab9 on their surfaces. We also describe distinct domains occupied by two late endosomal Rab GTPases: Rab9 and Rab7.
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Results |
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Expression of YFP-Rab9 and CFP-Rab7 in BS-C-1 cells revealed a very similar pattern of protein localization (Fig. 3, top), but remarkably the proteins were present in distinct regions. As seen before for GFP-Rab7 (Bucci et al., 2000), Rab7 was detected in perinuclear late endosomes. As shown in video 1 available at http://www.jcb.org/cgi/content/full/jcb.200109030/DC1 and Fig. 3 (bottom right), Rab7 and Rab9 were often associated with what appeared to be a single organelle that rotated and translated along microtubules without displaying significant mixing of the two GTPases. The red and green labels resembled two macaroon cookies sandwiched together. In addition, some organelles were positive for Rab7 or Rab9 but not both GTPases. Occasionally, a rim of overlap could be detected between the two domains. Thus, Rab7 and Rab9 appear to define distinct endosomal domains and are not always present on the same endosome.
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Under certain conditions, polyclonal antibodies can trigger receptor delivery to lysosomes, but a huge excess of antibody is required: for example, addition of 10% polyclonal antiCI-MPR antiserum for 5 h decreased receptor levels by 30% (Pfeffer, 1987). We instead added monoclonal antiCI-MPR IgG for 3 h at 20 µg/ml antibody, which did not seem to alter MPR trafficking (see below).
As shown in Fig. 5 A, fluorescent antiCI-MPR IgG was present in punctate structures throughout the cytoplasm that resembled early and late endosomes as expected. To determine the distribution of MPR IgG within late endosomes, we scored all Rab9- or Rab7-positive structures and then determined the percentage of those membrane domains that also housed antiCI-MPR IgG. Although 16% of Rab7-positive domains also contained anti-MPR IgG (n = 1,529), 40% of Rab9-positive domains were doubly positive for anti-MPR IgG (n = 370) (Fig. 5 B). These data show that CI-MPRs are enriched in Rab9-positive regions of late endosomes. Because it is unlikely that every molecule of CI-MPR will have an antibody molecule attached to it, our values are likely to represent an underestimate of the fraction of Rab9 domains that actually contain MPRs. In addition, our antibody labeling protocol will include MPRs en route to the late endosome from the cell surface. Thus, it is possible that MPRs in the Rab7 domain may be en route to the Rab9 domain.
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If TIP47 is required for Rab9 domain generation, expression of the mutant protein might generate aberrant endosomal structures by forming a nonfunctional Rab9-MPR-TIP47 assembly. To test this possibility, cells stably expressing YFP-Rab9 were transiently transfected with two plasmids: one encoding CFP to permit detection of transfected cells and a second plasmid encoding TIP47SVV-AAA (Carroll et al., 2001).
As shown in Fig. 6 A, expression of TIP47SVV-AAA often yielded significantly larger and unusual looking Rab9-positive structures (bottom) than seen in cells that did not express the mutant protein (top). In addition, cells expressing the TIP47 mutant protein endocytosed significantly less anti-MPR IgG (Fig. 6 B, middle compared with bottom or control panel at the top). This suggests that TIP47SVV-AAA has sequestered MPRs intracellularly, blocking both their recycling to the TGN (Carroll et al., 2001) and their ability to cycle via the plasma membrane. TIP47 has been shown already to colocalize with MPRs in fixed cells (Diaz and Pfeffer, 1998) and is present in late endosomes in mutant TIP47-expressing cells (unpublished data). Because the TIP47 mutant can influence the appearance of Rab9-positive compartments and sequester MPRs intracellularly, the simplest explanation would be that TIP47 is part of the Rab9- and MPR-enriched endosomal subdomain.
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We took two approaches to circumvent this problem. First, we treated cells with nocodazole to depolymerize microtubules and disperse both endosomes and the Golgi complex (Fig. 7 A). We have shown that endosome to trans-Golgi transport of MPRs does not require intact microtubules; intact microtubules enhance the initial rate but not the overall extent of transport (Itin et al., 1999). In the absence of nocodazole, vesicles moved with a velocity of 0.75 µm/s; upon microtubule depolymerization, this decreased to
0.2 µm/s (Fig. 7 B). Motility was bidirectional (video 2 available at http://www.jcb.org/cgi/content/full/jcb.200109030/DC1). Despite the loss of most high velocity motility in nocodazole-treated cells, some residual microtubules were seen as were Rab9-positive vesicles moving along them (video 3 available at http://www.jcb.org/cgi/content/full/jcb.200109030/DC1).
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The CFPgalactosyltransferase construct employed contains 81 NH2-terminal amino acids of human ß 1,4-galactosyltransferase, including the membrane-anchoring peptide that targets the fusion protein to the trans- and medial-Golgi (Llopis et al., 1998). Upon expression in BS-C-1 cells, this chimeric construct is also found in the trans-Golgi network because it could be shown to overlap with AP-1 at the TGN (unpublished data). Using immunogold EM, Llopis et al. (1998) reported colocalization of this fluorescent galactosyltransferase construct with the TGN marker, TGN38. This is important, because it has been shown previously that MPRs recycle to sialyltransferase-positive compartments (TGN) but not galactosyltransferase-positive compartments (trans-Golgi) of CHO cells (Duncan and Kornfeld, 1988).
CFP-galactosyltransferasepositive structures were distinct from YFP-Rab9 structures upon cotransfection of BS-C-1 cells (Fig. 8 A). This confirms our earlier conclusion that most of the Rab9 is present on late endosomes; a small amount may be on the TGN.
Close examination of these cells by video microscopy revealed vesicle transfers from Rab9-positive compartments to the trans-Golgi (Fig. 8 B; video 4 available http://www.jcb.org/cgi/content/full/jcb.200109030/DC1). The time-lapse series shown in the video reveals two successive vesicle-sized intermediates that appear to be fusing directly with what can be defined as the Golgi by virtue of its content of a bona fide Golgi marker. Both of the vesicles follow an identical approach pathway, which is likely provided by an unlabeled microtubule. Thus, these images capture the transfer of Rab9 vesicles from what is likely to represent a late endosome to the Golgi complex in real time. In addition, the data support the proposal that Rab9 remains vesicle associated throughout vesicle budding, docking, and less clear but possibly also, fusion events. The loss of red color from the Golgi just before or after fusion could be due to GDI-mediated removal of Rab9 from that compartment or alternatively could reflect Rab9 dilution in the target membrane bilayer. Indeed, the rapid loss of Rab9 staining on the target membrane was impressive.
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Discussion |
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We have shown for the first time that Rab9 is present on vesicles that bud off of Rab9-positive structures. In addition, small Rab9-positive vesicles can fuse with the Golgi complex. We do not yet know what proteins coat these vesicles, but a prediction of our model is that they bear TIP47 on their surfaces. In addition, Rab9 remains vesicle associated at least through the docking reaction and possibly until after the fusion process is complete.
We have shown that like early endosomal Rabs Rab9 occupies a discrete domain on late endosome membranes that include MPRs and is likely to also include other proteins needed for vesicle formation, docking, and fusion. Rab7 was generally adjacent to Rab9, although some vesicles were positive for one of the two Rabs and not the other. Because the two domains were so distinct, it remains possible that we are visualizing two discrete vesicles that are tightly attached to each other. However, their remarkably close association seen by video microscopy appeared more consistent with the Rabs occupying distinct domains in a single membrane-bound compartment. In either case, the Rabs define distinct membrane domains.
TIP47 is likely to enrich MPRs within the Rab9 domain. In support of this conclusion is our finding that a mutant TIP47 that binds poorly to Rab9 alters dramatically the appearance of Rab9-positive compartments. Our working model is that the mutant protein coassembles with native TIP47 to form an aberrant structure. In other work, we have shown that a myc-tagged TIP47 coassembles with endogenous wild-type TIP47. Such coassembled mutant complexes may sequester MPRs within late endosomes; indeed, cells expressing the TIP47 mutant endocytosed significantly less antiMPR-IgG than control cells and showed altered morphology of Rab9-positive compartments. Altogether, these data suggest that TIP47, Rab9, and MPRs are present altogether in an endosomal subdomain.
Do the transport vesicles we have visualized contain MPRs? MPR transport from endosomes to the Golgi complex is completely dependent on Rab9 function (Lombardi et al., 1993; Riederer et al., 1994). In addition, we have shown here that Rab9 domains from which the vesicles bud are enriched in CI-MPRs. Unfortunately, we could not get enough label onto MPRs to detect their presence in nascent vesicles containing Rab9 protein. In addition, little of the CFP-CD-MPR is ever seen in late endosomes at steady-state, making it unusable as a cargo marker for the retrograde transport route. It seems very possible that the CFP on the cytoplasmic domain of the CD-MPR may slow the export of this receptor from the TGN.
Although some vesicles might be formed that do not contain MPRs, the simplest model is that MPRs are indeed contained within the Rab9-positive vesicles. Because Rab9 enhances the affinity of TIP47 for MPR cytoplasmic domains, it seems reasonable to propose that these proteins are packaged altogether into transport carriers. In any event, we never saw tubules emerge from the Rab9 domain nor did we detect CI-MPRs in tubules emerging from Rab9-positive structures.
Rab7 is believed to function in the homotypic fusion of late endosomes (Feng et al., 1995; Bucci et al., 2000). This is distinct from the function of Rab9, which is needed for MPR recycling to the Golgi complex (Lombardi et al., 1993; Riederer et al., 1994). It will be of interest to determine if organelles positive for Rab9 or Rab7 (but not both) are capable of fusing with each other. If, like Rab5 in early endosomes, Rab7 organizes the homotypic late endosome fusion machinery, it is possible that the Rab9+ Rab7- organelles will have lost the capacity to fuse at high efficiency with the Rab7+ Rab9- organelles. Our experiments cannot rule out the presence of nonfluorescent Rab7 in a Rab9-positive compartment. Yet the observations suggest an unappreciated possibility that formation of a Rab9-positive Rab7-negative organelle would yield a terminal compartment that can no longer fuse with other late endosomes; it would only retain the capacity to bud off Rab9 vesicles or be a target for autophagic consumption. Therefore, late endosomes may have a mechanism to retain Rab7 and Rab9 in distinct domains within a single membrane-bound organelle to avoid such an outcome.
We have tried to construct functional fluorescent TIP47 chimeras using GFP, YFP, and CFP attached to either the NH2 or COOH terminus of the protein without success. We made second generation constructs that contained a polyglycine linker between the fluorescent tag and the TIP47 protein at either end. In all cases, the resulting fusion proteins failed to associate with membranes unlike native TIP47. We will not give up our attempts to generate a functional fluorescent TIP47 protein; we hope to use it to establish whether TIP47 is indeed present on transport vesicles and unlike Rab9 falls off of the vesicles before docking and fusion events. Our working model is that TIP47 collects Rab9 and MPRs into nascent transport vesicles; TIP47 release would then permit Rab9-GTP recruitment of yet to be discovered docking factors that mediate the subsequent events. We hope that future experiments will enable us to test this working model.
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Materials and methods |
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Cell culture, antibody uptake, and nocodazole treatment
African green monkey kidney epithelial (BS-C-1) cells were maintained in minimal essential medium supplemented with 7.5% FCS, penicillin, and streptomycin. To visualize endogenous CI-MPRs, cells were washed three times with PBS and incubated for 3 h at 37°C in complete media containing 20 µg/ml Texas red antiCI-MPR IgG prepared with Texas red sulfonyl chloride according to the manufacturer (2.4 moles Texas red per mole IgG; Molecular Probes). Cells were then chased for 15 min in complete medium; in some experiments, cells were fixed for 15 min in 3% paraformaldehyde before light microscopy. Nocodazole treatment was for 1 h at 37°C in complete medium containing 4 µg/ml nocodazole (Sigma-Aldrich).
Sucrose gradient flotation
BS-C-1 cells were grown on 100-mm plates and transfected with pEGFP-Rab9. After 1620 h, cells were washed three times with PBS and once with 10 mM Hepes, pH 7.4. Cells were then swollen for 15 min at 4°C in 10 mM Hepes, pH 7.4, supplemented with protease inhibitors (40 µg/ml aprotinin, 10 µg/ml leupeptin, 1 µM pepstatin A, and 1 µM phenylmethylsulfonyl fluoride). Cells were then harvested by scraping with a rubber policeman in 100 mM KCl, 1.5 mM magnesium acetate, 25 mM Hepes, pH 7.4, supplemented with 1 mM ATP and protease inhibitors. Cell extracts were loaded at the bottom of a nonlinear sucrose gradient (300 µl, 0.5 M sucrose, 300 µl 1.2 M sucrose, and 800 µl 2.5 M sucrose) and centrifuged for 90 min at 54,000 rpm at 4°C in a TLS-55 rotor (Beckman Coulter). Fractions were collected from the top, and equal aliquots were resolved by SDS-PAGE and transferred onto nitrocellulose. Protein distributions were determined by immunoblot using mouse anti-Rab9 culture supernatant (undiluted) and mouse anti-p115 ascites (diluted 1:750).
Live cell imaging and vesicle tracking
Cells grown on glass coverslips were observed 1620 h after transfection after mounting on a temperature-controlled stage. During observation, cells were maintained in phenol red-free DME containing 7.5% FBS buffered with 20 mM Hepes, pH 7.3, and covered with silicon DC-200 fluid (Serva). Observations were performed on a Nikon Diaphot-300 inverted microscope equipped with a cooled CCD camera (NDE/CCD; Princeton Instruments), and images were analyzed using Metamorph (Universal Imaging Corp.) software. FITC, rhodamine, and dual CFP/YFP filters were from Chroma Technology Corp. Simultaneous detection of CFP and YFP was performed using a dual emission filter by switching between excitation wavelengths with a filter wheel controlled by the Metamorph software. Vesicle motilities were determined at 37°C in cells transfected 1620 h using the manual tracking point application of Metamorph software and by averaging the distance moved in 1020 adjacent time intervals.
Immunofluorescence microscopy
Immunofluorescence was performed as described by Warren et al. (1984) using cells grown on glass coverslips. Mouse antiCI-MPR culture supernatant was used undiluted. Texas red or fluorescein isothiocyanate-conjugated secondary antibodies were used at 1:1,000.
Online supplemental material
Video 1 shows YFP-Rab9 (red) and CFP-Rab7 (green) in BS-C-1 cells 18 h after transfection. Images were acquired at room temperature to slow intracellular movement. Speed is 14x actual. In video 2, BS-C-1 cells 20 h after transfection with GFP-Rab9 were observed at 37°C. Bidirectional motility along microtubules can be observed (compare with cells treated with nocodazole; video 3). Speed is 5x actual. In video 3, BS-C-1 cells transfected with GFP-Rab9 were treated for 1 h with 4 µg/ml nocodazole. The video shows a Rab9-bearing vesicle budding off of an organelle and fusing with another organelle. Speed is 10x actual. In video 4, BS-C-1 cells 20 h after transfection with YFP-Rab9 (red) and CFP-galactosyltransferase (GalT'ase) (green) were observed at 37°C. The video shows YFP-Rab9bearing vesicles (arrow) fusing with a CFP-GalT'asepositive compartment. The video (12x actual speed) contains slowed sequences (3x actual). Videos 14 are available at http://www.jcb.org/cgi/content/full/jcb.200109030/DC1.
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Footnotes |
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* Abbreviations used in this paper: CFP, cyan fluorescent protein; GFP, green fluorescent protein; MPR, mannose 6-phosphate receptor; TGN, trans-Golgi network; YFP, yellow fluorescent protein.
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Acknowledgments |
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Submitted: 10 September 2001
Revised: 2 January 2002
Accepted: 2 January 2002
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References |
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