Botanisches Institut, Universität zu Köln, D-50931 Köln, Germany
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Abstract |
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The flagellar basal apparatus comprises the basal bodies and the attached fibrous structures, which together form the organizing center for the cytoskeleton in many flagellated cells. Basal apparatus were isolated from the naked green flagellate Spermatozopsis similis and shown to be composed of several dozens of different polypeptides including a protein band of 95 kD. Screening of a cDNA library of S. similis with a polyclonal antibody raised against the 95-kD band resulted in a full-length clone coding for a novel protein of 834 amino acids (90.3 kD). Sequence analysis identified nonhelical NH2- and COOH-terminal domains flanking a central domain of ~650 residues, which was predicted to form a series of coiled-coils interrupted by short spacer segments. Immunogold labeling using a polyclonal antibody raised against the bacterially expressed 95-kD protein exclusively decorated the striated, wedge-shaped fibers, termed sinister fibers (sf-fibers), attached to the basal bodies of S. similis. Striated fibers with a periodicity of 98 nm were assembled in vitro from the purified protein expressed from the cloned cDNA indicating that the 95-kD protein could be a major component of the sf-fibers. This structure interconnects specific triplets of the basal bodies with the microtubular bundles that emerge from the basal apparatus. The sf-fibers and similar structures, e.g., basal feet or satellites, described in various eukaryotes including vertebrates, may be representative for cytoskeletal elements involved in positioning of basal bodies/centrioles with respect to cytoskeletal microtubules and vice versa.
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Introduction |
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THE base of eukaryotic flagella is formed by specialized microtubular structures, basal bodies. The structure of the basal bodies, consisting of nine microtubular triplets, is highly conserved and also characteristic for
centrioles, which are part of the centrosome in many eukaryotic cells. During sexual and asexual reproduction, basal
bodies are often converted into centrioles, indicating their
functional similarity (Kellogg et al., 1994). It is generally
accepted that basal bodies/centrioles function as a template
for the structure of the axoneme (Lange and Gull, 1996b
).
An open question is whether or not centrioles contribute to the centrosome of animal cells, for example, by organizing its microtubule nucleation sites or by controlling its duplication.
The basal bodies of many flagellated cells not only serve
as assembly sites for the axoneme but also represent the
central structures of the basal apparatusa functional homologue of the centrosomal complex of animal cells
(Baron and Salisbury, 1992
). The basal apparatus consists
of the basal bodies and a diverse array of filaments attached to them. Sets of flagellar root microtubules usually
radiate from the basal apparatus and form the major part of the microtubular cytoskeleton (which is involved in the
determination of polarity, shape, and internal organization
of flagellated cells) (Moestrup, 1982
; Melkonian, 1984
;
Holmes and Dutcher, 1989
). Thus, analysis of the flagellar
basal apparatus could provide information about the basal
bodies/centrioles as centrosomal organizers.
In green flagellates like Chlamydomonas reinhardtii, two
types of the filaments present in the basal apparatus have
been analyzed in detail (Lechtreck and Melkonian, 1991a).
One is characterized by the presence of centrin, a 20-kD
protein belonging to the EF-hand family of calcium-binding proteins (Huang et al., 1988
). Centrin-based filaments
(system II fibers; Melkonian, 1980
) form a connection between the basal bodies, interconnect the basal bodies and
the cell nucleus, and are part of the flagellar transitional
region; they undergo calcium-induced contractions and
are involved in various motile functions of the basal apparatus (Melkonian et al., 1992
; Salisbury, 1995
). The other
well-characterized filaments of the basal apparatus are
the noncontractile, striated microtubule-associated fibers
(SMAFs,1 or system I fibers) (Melkonian, 1980
) that are
composed of striated fiber (SF)-assemblin, a highly
-helical 30-kD protein (Lechtreck and Melkonian, 1991b
; Weber et al., 1993
). These are attached to the four bundles of
flagellar root microtubules characteristic for Chlamydomonas-like flagellates and are thought to function as stabilizing elements in the basal apparatus (Lechtreck and Silflow, 1997
). Electron microscopical studies have revealed
additional filamentous structures attached to the basal
bodies in the green algal basal apparatus (Ringo, 1967
;
Beech and Melkonian, 1993
; Geimer et al., 1997a): transitional fibers (connectives between the basal bodies and
the plasma membrane), proximal plates or connecting fibers, and linkers between basal bodies and flagellar root
microtubules. The latter may establish the spatial relationship between the basal bodies and the flagellar root microtubules, as well as direct the microtubular roots to specific
locations in the cell. In contrast to centrin- and SF-assemblin-based fibers, these other fibers and linkers are relatively small, making it more difficult to identify their biochemical composition.
We have recently developed a method to obtain homogeneous preparations of basal apparatus from the naked,
biflagellate green alga Spermatozopsis similis (Geimer et al.,
1997b). The complex, filamentous system surrounding the
two basal bodies in the isolated state has been studied in
detail by serial thin section electron microscopy (Geimer
et al., 1997a). Besides the major proteins of the basal apparatus (tubulin, centrin, and SF-assemblin) more than two
dozen different polypeptides were present in our preparations. Here we identify a novel 95-kD protein as a component of the sinister fibers (sf-fibers), which are linkers between the basal bodies and two of the four microtubular
flagellar roots in the basal apparatus of S. similis. The protein contains extended coiled-coil domains and assembles
into striated fibers in vitro. The green algal sf-fibers resemble the basal feet that have been described in various eukaryotes (Gibbons, 1961
), and may be representative of a
particular type of basal body-microtubular flagellar root linker that is a common component of the cytoskeleton.
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Materials and Methods |
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Strains and Culture Conditions
Spermatozopsis similis (Sammlung von Algenkulturen Göttingen [SAG]
B 1.85; Schlösser, 1994) and Chlamydomonas reinhardtii cw 15+ (SAG
83.81) were maintained in aerated cultures (~1 liter × min
1) at 15°C with
a light-dark cycle of 14:10 h and a photon flux density of 20 µEinstein (E) × m
2 × s
1 (Osram 40W/25 universal white) in Waris medium (McFadden and Melkonian, 1986
).
Isolation of a 95-kD Protein Band from the Basal Apparatus
The isolation and purification of basal apparatus of Spermatozopsis similis
has been described (Snell et al., 1974; Geimer et al., 1997b
). Briefly, concentrated cells were lysed with 1.5% Triton X-100 in TE buffer (10 mM
Tris-HCl, pH 7.4, 2 mM EDTA), disintegrated by homogenization,
washed with TE buffer/0.5 % Triton X-100 (800 g, 30 min), and then the
suspension was loaded onto a discontinuous sucrose gradient (6 ml 60%,
9 ml 50%, 9 ml 40%, and 6 ml 20% in TE buffer/0.1% Triton X-100) and
centrifuged for 1 h at 12,500 g in a swing-out rotor (HB-4; Sorvall, Bad
Homburg, Germany). Highly enriched basal apparatus were collected
from a milky band below the 20-40% sucrose interphase, and the proteins were separated by preparative SDS-PAGE. A band of 95 kD was excised from Coomassie blue-stained gels, concentrated by electrophoresis, and
then blotted onto nitrocellulose membrane. The membrane with ~10 µg
of the 95-kD band was dissolved in dimethyl sulfoxide, mixed with complete Freunds adjuvant, and subcutanously injected at several sites into a
young, male rabbit. For subsequent booster injections on day 15 and 53, we used acrylamide gel pieces with ~10 µg antigen, homogenized in PBS,
and then mixed with incomplete Freunds adjuvant. An IgG fraction (anti-p95) was purified from the serum taken 10 d after the second injection using a fast protein liquid chromatography (FPLC) system (Pharmacia LKB,
Uppsala, Sweden) and a protein A column (Pharmacia LKB).
Cloning and Sequencing of a cDNA Coding for the 95-kD Protein
Total RNA from S. similis was isolated by the phenol/SDS method basically following the protocol of Palmiter (1974). To purify mRNA, a self-packed oligo-dT column (Boehringer Mannheim GmbH, Mannheim,
Germany) was used. A Uni-Zap XR cDNA library was constructed using
a cDNA synthesis and cloning kit (Stratagene, Heidelberg, Germany) according to the manufacturer's instructions with the following modifications: first-strand synthesis was done for 15 min, 45°C; 20 min, 50°C; and
20 min, 55°C, with reverse transcriptase from GIBCO BRL (Eggenstein,
Germany). The library was screened with anti-p95, and positive clones
were analyzed by restriction mapping and partial sequencing after in vivo
excision of the pBluescript phagemid. In addition, bacterial lysates obtained from isopropyl-
-D-thiogalactopyranoside (IPTG)-induced cultures were analyzed with anti-p95 in Western blots. A 3.1-kb clone (clone
7/1) produced an immunoreactive band of 95 kD. The insert was sequenced completely in both directions (Abiprism, 310 Gene Analyzer;
Perkin Elmer Applied Biosystems, Weiterstadt, Germany). The AutoAssembler 1.4.0 (Perkin Elmer Applied Biosystems) program was used for
gene assembly and the sequence was analyzed using programs (COILS,
FASTA, BLAST) provided by European Bioinformatics Institute (EBI;
http://www.ebi.ac.uk/services/services.html) and Baylor College of Medicine (BCM; http://dot.imgen.bcm.tmc.edu:9331/seq-search/struc-predict.html) via the internet (Altschul et al., 1990
; Lupas, 1996
).
Purification of the Recombinant 95-kD Protein and Antibody Production
For induction of gene expression, Escherichia coli XL-1Blue cells containing clone 7/1 were grown at 37°C to a density of OD600 0.8-1.0 and induced
with 2 mM IPTG. After 4 h the cells were harvested (1,000 g, 15 min, 4°C),
resuspended in 50 mM NaH2PO4, 300 mM NaCl, pH 7.8, and frozen at
80°C for 20 min. After thawing, the bacteria were sonicated five times
for 10 s, and the insoluble material was pelleted by centrifugation (10,000 g,
10 min, 4°C). The pellet was washed three times (10,000 g, 10 min, 4°C) in
washing buffer (50 mM Tris-HCl, 1 mM EDTA, 3% Triton X-100, 0.05%
-mercaptoethanol, pH 7.5) and finally twice in 50 mM Tris-HCl, pH 7.5. Then, the pellet was resuspended in 20 mM Tris-HCl, 2 mM EDTA, 1 mM
DTT, pH 8.0, containing 8 M urea and extracted for 2 h at room temperature. To remove insoluble material the suspension was centrifuged at 18,000 g for 30 min and filtered (0.22 µm, Millex-GV; Millipore, Eschborn, Germany). The proteins were separated on a MonoQ column (HR
5/5, Pharmacia LKB) equlibrated with the urea buffer using a FPLC system (Pharmacia LKB). The column was eluted with a linear salt gradient
(0-50 mM KCl) in the same solvent and fractions containing the 95-kD
protein were identified by SDS-PAGE. MonoQ fractions of interest were
applied to preparative SDS-PAGE. For internal or NH2-terminal amino
acid sequencing, the protein was transferred to polyvinylidene difluoride
membrane. For antibody production the recombinant 95-kD protein was
cut out, reelectrophorized, and electroeluted. The protein concentration
was determined using the method of Neuhoff et al. (1979)
, and four injections of 75 µg of homogenous 95-kD protein each were used to generate
polyclonal antibodies (anti-rec95) in rabbit (as above or by BioGenes,
Berlin, Germany) (day 1, intralymphatic; day 7, intramuscular; day 14, subcutan; day 29, intramuscular).
Reassembly of Striated Fibers from the Recombinant 95-kD Protein
MonoQ fractions containing the 95-kD protein (>95% pure, ~0.1 µg/µl) were dialyzed using a floating membrane filter (type VS, pore size 0.025 µm; Millipore) against an excess amount of 10 mM Tris-HCl, 1 mM EDTA, 150 mM KCl, pH 7.0, with decreasing concentrations of urea (4 M, 2 M, 0.8 M, and 0 M) for 120 min at each urea concentration. An alternative protocol used dialysis tubes (12,000-14,000 D; Medicell, London, UK) and prolonged dialysis times (4 h) for each concentration. Reassembled fibers were analyzed by whole mount electron microscopy. The fibers were harvested by centrifugation (100,000 g, 30 min), the proteins in the supernatant were precipitated by the addition of acetone (1:9), and both fractions analyzed by SDS-PAGE.
Immunoblot Analysis
Whole cells, isolated cytoskeletons, or enriched basal apparatus of S. similis were separated by SDS-PAGE and transferred to PVDF membrane (Millipore Corp., Bedford, MA). After extensive blocking with PBS-T (PBS with 0.05% Tween 20) containing 3% gelatin from cold water fish (Sigma Chemical Co., St. Louis, MO) the membrane strips were incubated with the primary antibody (anti-p95, 1:500 or anti-rec95, 1:1,000 in PBS-T/3% gelatin) for 60 min. The membrane was washed five times for 15 min with PBS-T, and then blocked again for 30 min, before incubation with anti- rabbit IgG peroxidase conjugate (Sigma Chemical Co.; 1:1,000 in PBS-T/3% gelatin) for 60 min. Membrane strips were washed five times for 15 min in PBS and developed using 4-chloro-1-naphthol as the substrate.
Indirect Immunofluorescence
The preparation of isolated cytoskeletons of S. similis for indirect immunofluorescence and antibody staining has been described previously (Lechtreck
and Melkonian, 1991b). The anti-p95 and anti-rec95 sera were used in a
1:100 dilution; monoclonal anti-
-tubulin was applied simultaneously. Secondary antibodies (anti-rabbit IgG or anti-mouse IgG conjugated to FITC
or TRITC, Sigma Chemical Co.) were diluted 1:50. Immunofluorescence
images were observed using a Zeiss IM35 equiped with a 1,3/100× oil
immersion objective (Carl Zeiss, Oberkochen, Germany), documented on
HP5 (Ilford Ltd., Knutsford, UK), digitized, labeled, and assembled using
Photoshop (Adobe Systems Inc., Mountain View, CA) and Powerpoint
(Microsoft, Redmond, WA).
Preembedding Immunogold Electron Microscopy
Cells of S. similis were washed by centrifugation in MT (30 mM Hepes, 5 mM EDTA, 15 mM KCl, pH 7) supplemented with 5 mM MgSO4 (MT-Mg2+) and lysed by the addition of an equal volume MT-Mg2+/2% Triton
X-100, immediately followed by fixation with 1% paraformaldehyde (final
concentration in MT-Mg2+) for 5 min. The cytoskeletons were washed
twice in the presence of the fixative (80 g, 10 min, 15°C). All subsequent
steps were performed as described previously (McFadden et al., 1987).
The anti-rec95 was used at a 1:200 final dilution and detected with protein
A conjugated to 15-nm gold particles. Samples were transferred to agar,
dehydrated, and then embedded in Epon 812 (Serva, Heidelberg, Germany) as previously described (McFadden and Melkonian, 1986
).
Standard and Whole Mount Electron Microscopy
For whole mount electron microscopy, 5 µl of suspended particles were
applied to copper grids coated with pioloform (Plano GmbH, Marburg,
Germany) and allowed to adhere for 10-60 min. The grids were stained
with 1% aqueous uranyl acetate for 1-2 min. Utrathin sections were cut
with a MT-6000 microtome (RMC, Tucson, AZ) and stained with uranyl
acetate and lead citrate (Reynolds, 1963). Micrographs were taken with a
transmission electron microscope (CM 10; Philips Electron Optics, Mahwah, NJ) using Scientia EM Film (Agfa, Leverkusen, Germany).
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Results |
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Identification of 95-kD Components in the Basal Apparatus
Spermatozopsis similis is a naked, biflagellate green alga
that resembles Chlamydomonas reinhardtii in its major ultrastructural features. Detailed descriptions of the cells,
isolated cytoskeletons, and basal apparatus are given in
Preisig and Melkonian (1984), Lechtreck et al. (1989)
, and
Geimer et al. (1997a). A uniform terminology for the
structures in the basal apparatus of green flagellates has
now been established (Moestrup and Hori, 1989
; Beech et al.,
1991
), and the developmentally oldest basal body is designated as basal body No. 1, and the suffixes "s" (for sinister (Latin) = left) and "d" (for dexter = right) are used for
the associated structures according to their position relative to basal body No. 1 (see Fig. 9). The four microtubular
roots of the cruciate x-2-x-2 flagellar root system (where x = 3-6 microtubules), which are characteristic for Chlamydomonas-like flagellates, are called 1s (x-root of basal body
No. 1) and 1d (two-stranded microtubular root of basal
body No. 1), or 2s and 2d if associated with basal body No.
2. The fibrous structures that connect the basal bodies to
the microtubular roots are called 1sf, 1df, 2sf, and 2df, respectively (Beech and Melkonian, 1993
). The dominant
proteins in preparations of isolated basal apparatus of S. similis were tubulin, centrin, and SF-assemblin (Geimer et
al., 1997b
; Fig. 1 a). Several proteins with molecular
weights >50 kD were enriched in fractions of basal apparatus purified by sucrose gradient centrifugation in comparison to crude fractions. Most of these proteins constitute only 1% or less of the total protein; however, a band
of 95 kD was present comprising 2-4% of the proteins in
purified basal apparatus (Fig. 1 a). The 95-kD band was
purified by SDS-PAGE and used to raise a polyclonal antibody (anti-p95). When isolated cytoskeletons of S. similis
were stained with anti-p95 for indirect immunofluorescence, the region of the basal apparatus was labeled (Fig.
6, a and b). In Western blotting experiments, this antibody
labeled two closely spaced bands of 95 kD, which were notably enriched in the basal apparatus in comparison to
whole cells or isolated cytoskeletons (Fig. 1 b).
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Cloning of the 95-kD Protein
SDS-PAGE analysis, indirect immunofluorescence and Western blotting analysis indicated that the antigens recognized by anti-p95 were components of the basal apparatus of S. similis (Figs. 1 and 6). Screening of a S. similis cDNA expression library with anti-p95 resulted in three clones with inserts of 3.1 kb and two shorter variants (1.9 and 2.3 kb), all with identical 3' sequences. Both strands of one of the 3.1-kb clones (clone 7/1) were completely sequenced. The sequence contained a large open reading frame of 2,505 bp that had a GC content of 75%; the putative initiation codon was preceded by an in-frame stop codon (Fig. 2). An immunoreactive 95-kD protein was present in bacteria containing clone 7/1 after induction with IPTG (see Fig. 4, a, lane 2, and b), demonstrating that the clone is full length. Southern blot analysis using the insert of clone 7/1 as a probe indicated that the protein is encoded by a single copy gene in S. similis (data not shown).
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Sequence Analysis
The ORF of clone 7/1 encodes a protein of 834 amino acids that has a predicted isoelectric point of 7.4 and a mass
of 90.3 kD (Fig. 2). Sequence analysis using the algorithm
of Garnier et al. (1978) identified a central domain of
~650 residues that is predominantly
-helical (~88%),
flanked by nonhelical NH2- and COOH-terminal domains
with lengths of ~65 and 120 residues, respectively. The
program COILS (Lupas, 1996
) predicted a high probability of the central domain forming coiled-coil structures separated by short spacers around residues 140, 270, 450, and 620 (Fig. 3 a). Because of the presence of heptad repeats, two leucine zipper motifs (L-6X-L-6X-L-6X-L in
position 392-413 and 572-593) were identified by PROSITE. The head and tail domains contained all prolines
(14 of 17 located in the COOH-terminal domain) and five
SPS(K/R) motifs (four in the COOH-terminal domain), which resemble the known consensus sequences for p34cdc2
kinase (Moreno and Nurse, 1990
; Figs. 2 and 3 b). Database searches using the complete amino acid sequence of
the 95-kD protein detected only low (<25%) homologies
to other proteins with large coiled-coil domains (e.g., myosin, paramyosin, and intermediate filament proteins). In
comparison with those proteins, the 95-kD protein displays a high content of alanine (19.7%), whereas isoleucine (1.4%) is underrepresented. This might reflect the
high GC-content of the cDNA of the 95-kD protein (e.g.,
isoleucine has the codons AT[A/T/C]; 161 alanines are encoded by GC[C/G], but only three by GC[A/T]).
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Purification of Recombinant 95-kD Protein and Antibody Production
Electrophoretic analysis of bacterial lysates showed that large quantities of recombinant 95-kD protein were present in the insoluble fraction (Fig. 4 a, lanes 2 and 3). The 95-kD protein was further purified by anion exchange chromatography and used for amino acid sequencing and reassembly experiments (Fig. 4 a, lane 4). NH2-terminal sequencing of the 95-kD protein identified two staggered sequences starting with methionine 1 or 4 (Fig. 2). Recombinant 95-kD protein (Fig. 4 a, lane 5) was used to raise a polyclonal antibody (anti-rec95). In Western blotting experiments this antibody recognized only the upper of the two bands labeled by the original anti-p95 (Fig. 4 b, lanes 4 and 5). In the meantime, immunoscreening of a cDNA library with anti-p95 yielded a clone different from clone 7/1 as revealed by partial sequences. Bacteria transformed with this clone expressed a protein of 95-kD that cross- reacts with anti-p95 but not with anti-rec95 (data not shown). We conclude that anti-p95 was heterospecific and that clone 7/1 encodes one of the two antigens of anti-p95.
Reassembly Experiments with Recombinant 95-kD Protein
Purified recombinant 95-kD protein (>95%) dissolved in 8 M urea was dialyzed successively against buffers with 4, 2, 0.8, and 0 M urea. EM analysis of the final dialysate showed the formation of striated fibers with a periodicity of 98.2 ± 5.6 nm (n = 20; Fig. 5, a and b). Correspondingly, the post-centrifugation pellet was almost entirely composed of 95-kD protein (Fig. 4 c, lane 2). Structurally, the reassembled fibers showed repeats consisting of a broad electron-translucent band (~60 nm) divided by an electron-opaque line of ~6 nm and an electron-opaque band (~40 nm; Fig. 5 b). The 95-kD protein formed thick, short fibers (length 643 ± 358 nm, n = 16; width 106 ± 61 nm, n = 15) often with tapered ends (Fig. 5 a). We sometimes observed ladder-like fibers with a periodicity of ~100 nm, consisting of alternating loose regions formed by thin filaments and condensed regions (Fig. 5, c and d); these may represent incompletely assembled intermediates. We also observed clusters of rod-like particles (average length of 81 nm [± 18 nm, n = 16] and a diameter of 2-3 nm; Fig. 5 e), which may represent oligomeric precursors of the striated fibers and also reflect the elongated shape of the 95-kD molecules. Reassembly experiments using dialysis tubing instead of microdialysis membrane (see Materials and Methods) or higher protein concentrations (>0.4 µg/µl instead of 0.1 µg/µl) resulted in the formation of electron-dense, spindle-shaped protein aggregates (length 557 ± 55 nm, n = 15; width 243 ± 77 nm, n = 12), which were sometimes cross-striated with a periodicity of 16 nm (n = 3; Fig. 5, f and g).
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Localization of the 95-kD Protein
Immunofluorescence staining of isolated cytoskeletons of S. similis with anti-rec95 resulted in comparatively weak labeling of two dots, frequently of unequal size, in the region of the basal apparatus (Fig. 6, c, d, and f). To localize the 95-kD protein more precisely, we performed immunogold electron microscopy (preembedding technique) on isolated cytoskeletons from S. similis (Fig. 7). In samples treated with anti-rec95, cone-like structures associated with the basal bodies were decorated (Fig. 7, a and f). The immunoreactive structures were identified as the sf-fibers that link the basal bodies with the five-stranded microtubular roots (s-roots). The intense labeling of the exposed surface was most clearly seen in cross-sections through the sf-fibers (Fig. 7, h and i). Previously, we have shown that the two sf-fibers present in a basal apparatus differ in size depending on their association with the developmentally older (basal body No. 1) or younger (No. 2) basal body (Geimer et al., 1997a; Fig. 8). Immunogold labeling of the small 2sf-fiber attached to basal body No. 2 is depicted in Fig. 7, c and d; sections through the prominent 1sf-fiber are shown in Fig. 7, a, b, and e-i. Extensive analyses of immunolabeled cytoskeletons revealed no specific reaction of anti-rec95 with any other component of the basal apparatus and preimmune controls showed no specific labeling of the sf-fibers or any other parts of the cytoskeleton (Fig. 7, b and g). The sf-fibers were also decorated by immunogold labeling using anti-p95 (data not shown). We conclude that the sf-fibers are the primary location of the 95-kD protein in the basal apparatus of S. similis.
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Structure of the sf-Fibers
The sf-fibers connect each of the two basal bodies with the
corresponding bundle of five microtubules. The latter are
part of the cruciate microtubular flagellar root system
characteristic for Chlamydomonas-like flagellates, which
in the case of S. similis, consist of two two-stranded (d-type)
and two five-stranded (s-type) microtubular bundles (Preisig and Melkonian, 1984). The 1sf-fiber is attached to basal
body No. 1 in a subdistal position and extends about 200 nm
towards the s-type root (Fig. 8, a and b). The connection to
the basal body involves 1-3 triplets depending on the level of section (Fig. 7, e and f; Fig. 8 b), and the junctions with at least some of the basal body triplets are formed by several thin filaments (Geimer et al., 1997a). Similar filaments
are present between the part of the sf-fiber associated with
the basal body and its distal part which overlies the flagellar root microtubules, suggesting a flexible junction (Fig. 8
a). The 1sf-fiber exhibits a cross-striation consisting of broad
electron-translucent and electron-opaque bands (Fig. 8, a
and b). The 2sf-fiber resides in a proximal position on
basal body No. 2 and is only ~80-nm long (Fig. 8 c). For a
detailed analysis of the structure of the sf-fibers by serial
ultrathin sections, see Geimer et al. (1997a). After intensive
mechanical disintegration of basal apparatus, we still observed complexes of basal bodies and attached sf-fibers, indicating that the 1sf-fibers are linked firmly to the basal bodies (Fig. 8, d and e).
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Discussion |
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We have identified a 95-kD protein as a component of the basal apparatus of the green flagellate Spermatozopsis similis. Analysis of the deduced sequence of the 95-kD protein showed a high probability for the formation of extended coiled-coils. The 95-kD protein forms rod-shaped particles in vitro and is located in the sf-fibers, which connect the basal bodies with the two five-stranded microtubular roots. Furthermore, fibers reconstituted in vitro from the recombinant 95-kD protein resembled the sf-fibers in that they had a cross-striation pattern consisting of broad electron-translucent and electron-opaque bands. We conclude that the 95-kD protein is the major structural component of the sf-fibers in S. similis.
Until now there are only very limited data about the distribution in other organisms of proteins homologous to the
95-kD protein from S. similis: no specific labeling was observed in various green algal (Dunaliella bioculata, Polytomella agilis), protist (Tetrahymena), or animal cells (sea
urchin sperm, 3T3 cells, rod cells of bovine retina, mouse
trachea). However, the antisera raised against the 95-kD
protein decorate two dots in the basal apparatus of C. reinhardtii in indirect immunofluorescence (not shown). A
possible explanation for the low species cross-reactivity of
the antibodies to the 95-kD protein could be the low sequence conservation characteristic for many coiled-coil
proteins. This is also true for SF-assemblin, the major protein of the noncontractile striated roots in green flagellates, which has only 54% identity of the amino acid sequence between S. similis and C. reinhardtii (Lechtreck
and Silflow, 1997), but obviously has homologues in organisms as phylogenetically distant as Giardia lamblia
(Weber et al., 1993
).
The sf-fibers are the third nontubulin filament system
identified in the green algal basal apparatus (Fig. 9). They
are distinct from the previously characterized centrin- and
SF-assemblin filaments in their biochemical composition
and putative function (Salisbury et al., 1984; Lechtreck
and Melkonian, 1991a
). Centrin fibers maintain the spatial
orientation of the basal bodies to each other and to the nucleus. Further, centrin may be involved in controlling basal
body replication (Taillon et al., 1992
). Because centrin fibers are contractile, they also serve to reorientate the
basal bodies (e.g., during reversal of swimming direction),
to decrease the distance between the basal apparatus and
the nucleus, or to sever microtubules during flagellar autotomy (McFadden et al., 1987
; Salisbury et al., 1987
; Sanders and Salisbury, 1994
). SMAFs run parallel to the microtubular roots and may stabilize the flagellar root microtubules
and absorb any mechanical stress caused by the flagellar
beat (Lechtreck and Melkonian, 1991b
). The sf-fibers, finally, link the basal bodies to the five-stranded microtubular roots (s-roots; Beech and Melkonian, 1993
). Functionally,
the sf-fibers could determine the position of the microtubular roots with respect to the basal bodies and guide the
s-roots into a specific direction. This seems important with
respect to the stereotyped internal order characteristic for
most flagellate cells that is thought to be maintained by
the microtubular flagellar roots (Melkonian, 1984
). Previous studies in green algae have shown that the 2s-root connects the eyespot apparatus with the corresponding basal
body (Holmes and Dutcher, 1989
; Lechtreck et al., 1997
).
Thus, the eyespot is in a defined position with respect to
the plane of flagellar beat, a situation important for phototaxis (Kreimer, 1994
). Further, the two s-roots often persist during mitosis and form a specialized structure, which
in Chlamydomonas is known as the metaphase band
(Johnson and Porter, 1968
). The s-root microtubules interconnect the dividing basal apparatus in an antiparallel
fashion and arc over the mitotic spindle (Gaffal and el-Gammal, 1990
). Thus, the metaphase band and homologous structures present in other green flagellates (Segaar
and Gerritsen, 1989
; Lechtreck et al., 1997
) may be involved
in the separation of the duplicated basal apparatus and the
positioning of the spindle apparatus and the cleavage furrow (Ehler et al., 1995
). Another important feature of the
sf-fibers is the difference in size between the two fibers present in the basal apparatus of S. similis. The 1sf-fiber is much more prominent than its counterpart at the younger
basal body resulting in a rather unequal distribution of the
95-kD protein to the two basal bodies. A similar distribution has been described for cenexin, a 96-kD protein of unknown primary structure, that is located exclusively in the
pericentriolar material surrounding one of the two centrioles in the centrosome of mammalian cells (Lange and
Gull, 1995
). Interestingly, as described for cenexin, the
prominent sf-fiber is a marker for the older basal body/
centriole. Furthermore, monoclonal antibodies directed
against Spc110p from the yeast spindle pole body identified an immunologically related 100-kD protein at the
proximal end of centrioles of mammalian cells, sometimes
more intensely decorating the parental centriole (Tassin et
al., 1997
). In summary, the link formed by the sf-fibers between the basal bodies and the microtubular s-roots may
establish the spatial relationship of these microtubular
roots to other cellular structures and the plane of flagellar
beat. In general, the decoration of specific microtubular
triplets of the basal body with fibrous structures like the sf-fibers could enable the basal bodies to act as the organizing center for the cytoskeleton of flagellate cells.
Fibrous structures (e.g., satellites, basal feet, appendices) that are similar to the sf-fibers have been described in
association with basal bodies and centrioles in various
metazoan cells (Gibbons, 1961; Reese, 1965
; Wolfe, 1972
;
Dentler, 1987
; Sandoz et al., 1988
; Paintrand et al., 1992
;
Lange and Gull, 1996a
). Basal feet are striated, champagne cork-shaped structures attached to two or three
triplets of the basal bodies in a subdistal, median, or proximal position. Basal feet as sf-fibers are pointing in the direction of the effective flagellar stroke, indicating that they
may be attached to a similar if not identical group of triplets (triplet Nos. 4, 5, and 6 according to the nomenclature
of Afzelius, 1959
; and Hoops and Witman, 1983
) of the
basal body, and thus could be markers for the lateral
asymmetry of basal bodies (Gibbons, 1961
). In diseases resulting in the immotile cilia syndrome, the basal feet of ciliated epithelia show a random disposition (Afzelius, 1980
).
Basal feet, sf-fibers, and satellites connect the basal bodies/centrioles with cytoskeletal microtubules (Vorobjev
and Chentsov, 1982
; Baron and Salisbury, 1988
). Whereas
most of the microtubules radiating from the mammalian
centrosome are nucleated in the pericentriolar material,
some are attached with their minus ends to the centriole-associated satellites or the centriole itself (Rieder and
Borisy, 1982
; Rattner, 1992
). It has been speculated that
these centriolar microtubules may function in anchoring
and positioning of the centrosomal complex (Rattner,
1992
). Only a few studies deal with the biochemical nature
of basal feet or satellites. Recently, basonuclin, a zinc finger protein located in the nuclei of epidermal basal cells,
has been localized to satellites of the flagellated centriole/
basal body in spermatocytes/spermatids (Yang et al., 1997
).
In PtK2 cells antibodies directed against algal centrin decorated the basal feet in addition to other centrosomal structures but cross-reacted with a protein of 165 kD in Western blotting (Baron and Salisbury, 1988
). The 95-kD protein
from S. similis described in this study is specifically located
in a linker between basal bodies and cytoplasmic microtubules. Future analysis of the 95-kD protein may provide
insights how the spatial relation between microtubules and
basal bodies/centrioles is established and maintained.
![]() |
Footnotes |
---|
Received for publication 11 July 1997 and in revised form 29 December 1997.
This work is dedicated to the memory of Annette Teltenkötter (2 May 1997).We thank K. Weber (Max-Planck Institute for Biophysical Chemistry, Göttingen, Germany) for the determination of peptide sequences, and M. Haring (BioCentrum, University of Amsterdam, Netherlands) for advice during the preparation of the cDNA library.
This study was supported by the Deutsche Forschungsgemeinschaft (DFG; Bonn, Germany).
![]() |
Abbreviations used in this paper |
---|
IPTG, isopropyl--D-thiogalactopyranoside;
sf-fibers, sinister fibers;
SMAF, striated microtubule-associated
fibers.
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References |
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