Cancer Research Campaign, Cell Cycle Genetics Group, Department of Anatomy and Physiology, University of Dundee, Dundee DD1 4HN, Scotland
abnormal spindle, a gene required for normal spindle structure and function in Drosophila melanogaster, lies immediately adjacent the gene tolloid at 96A/B. It encodes a 220-kD polypeptide with a predicted pI of 10.8. The recessive mutant allele asp1 directs the synthesis of a COOH terminally truncated or internally deleted peptide of ~124 kD. Wild-type Asp protein copurifies with microtubules and is not released by salt concentrations known to dissociate most other microtubule-associated proteins. The bacterially expressed NH2-terminal 512-amino acid peptide, which has a number of potential phosphorylation sites for p34cdc2 and MAP kinases, strongly binds to microtubules. The central 579-amino acid segment of the molecule contains one short motif homologous to sequences in a number of actin bundling proteins and a second motif present at the calmodulin binding sites of several proteins. Immunofluorescence studies show that the wild-type Asp protein is localized to the polar regions of the spindle immediately surrounding the centrosome. These findings are discussed in relation to the known spindle abnormalities in asp mutants.
Mutations in abnormal spindle (asp)1 lead to defects in mitosis at a variety of developmental
stages as well as in male meiosis. The late larval-pupal lethality seen with strong hypomorphic alleles of asp is typical of mutations in Drosophila genes encoding
stable proteins essential for mitosis. Homozygous individuals receive sufficient maternal gene product from their
heterozygous mothers to permit normal embryonic development to proceed. However, there is insufficient functional gene product to permit mitosis during larval and pupal stages, when the imaginal discs and abdominal histoblast
nests proliferate to form adult structures. Rare escapers may display a number of cuticular abnormalities associated with cell death, such as missing bristles, misshapen
tergites and sternites, and roughened eyes. The maternal
contribution of Asp protein has been demonstrated directly in cuticular clones in which there has been the loss
of a marked Y chromosome as a result of nondisjunction due to the mutation (Carmena et al., 1991 The asp locus was originally identified through a late
larval lethal mutation that causes defects in both mitosis
and meiosis (Ripoll et al., 1985 Animals transheterozygous for particular mutant alleles
of asp show an increased frequency of survival to adulthood (Gonzalez et al., 1990 Taken together, the various phenotypes seen in asp homozygotes suggest that the asp gene product may be involved in some aspect of spindle microtubule dynamics. In
this paper we show that asp encodes a microtubule-associated protein that localizes to the polar regions of the spindle early in mitosis and the midbody at telophase.
Microcloning DNA from the asp Region
Chromosomal material corresponding to polytene map position 96A2196B10 was microdissected from Oregon R third instar larval salivary gland
polytene chromosomes. Two fragments were microdissected from two
chromosomes and were pooled. The DNA was extracted and cloned, as described (Scalenghe et al., 1985 Chromosome Walking
Genomic clones were isolated from lambda libraries in Lambda dash
(Stratagene, La Jolla, CA), and cosmid libraries were those constructed
using the vectors Smart2 (Speek et al., 1988 cDNAs were subsequently isolated by using segments of the walk to
screen a testis cDNA library, kindly provided by T. Hazelrigg that had
been constructed by a combination of random and oligo-dT priming in the
vector P element-mediated Rescue of asp1 Mutants
Rescue experiments were carried out using two Drosophila melanogaster
lines containing homozygous second chromosome insertions of the P element transformation vector plasmids pMBO1366 and pMBO1367, respectively (Shimell et al., 1991 Sequence Determination and Analysis
Sequencing was carried out using the dideoxynucleotide chain termination procedure (Sambrook et al., 1989 Expression of Asp Protein in Escherichia coli and
Raising Polyclonal Antibodies
Plasmid pASP36 was constructed by inserting the NcoI-BamHI fragment
from p6a (see Fig. 2) into the expression vector pET23d (Invitrogen Corp.,
San Diego, CA). Conditions for culture and induction were as described
by the supplier. The polypeptide expressed by this clone represents the
NH2-terminal portion of the Asp protein and does not include the putative
actin binding domain. This polypeptide was insoluble and was purified by
preparing inclusion bodies, separating the proteins by polyacrylamide gel
electrophoresis, excising the appropriate band from the gel, and electroeluting the protein as described by Leppard et al. (1983)
Plasmid pASP11 was constructed by inserting the BamHI-EcoRI fragment from p6a (see Fig. 2) into the expression vector pQE31 (Qiagen,
Inc., Santa Clara, CA). Conditions for culture, induction, and denaturing
purification on Ni-agarose columns (Qiagen, Inc.) were as described by
the supplier.
Embryo Staining
Embryos were fixed and stained as described by Gonzalez and Glover
(1993) Microtubule Preparation
Microtubules were purified from 0-3-h-old Drosophila embryos essentially as described by Goldstein et al. (1986) Overlay Assays
Proteins were blotted to PVDF membranes (Amersham Life Sciences,
Pittsburgh, PA) after SDS-PAGE. Membranes were blocked with TBST
(50 mM Tris/HCl, pH 7.5, 150 mM NaCl, 0.05% Tween 20) containing 5%
nonfat dry milk for 1 h, washed for 15 min in TBST plus 1% nonfat dry
milk, and washed for 15 min in PEMF (0.1 mM Pipes/NaOH, pH 6.6, 1 mM
EGTA, 1 mM MgSO4, 25 mM NaF) buffer plus 1 mM GTP. The blots
were then incubated overnight at room temperature with 5 µg/ml Drosophila tubulin (purified as described) in PEMF supplemented with 1 mM
GTP and 20 mM taxol. The bound microtubules were detected by standard Western blot procedures using the monoclonal antibody Bx69 diluted five times.
Molecular Cloning of abnormal spindle
Previous genetic mapping studies (Gonzalez et al., 1989
To locate the asp gene, restriction fragments from the
chromosome walk were used to screen a Drosophila testis
cDNA library (a gift of Hazelrigg, T.). Genomic DNA corresponding to partial cDNA clones obtained from this screen
was used in a secondary screen of an embryonic cDNA library (Brown and Kafatos, 1988 Furthermore, this gene has a developmental pattern of
expression typical of many genes essential for the cell cycle whose products have to be maternally provided to the
embryo. We used EcoRI-EcoRI or NcoI-EcoRI fragments from the 6.5-kb cDNA clone p6a as hybridization
probes on developmental Northern blots. One transcript
of ~6.5 kb was readily observed (Fig. 2) in mRNA prepared from embryos and females (Fig. 2, lanes 1, 2, and 6)
but was present at greatly reduced levels in larval and
adult male mRNA (Fig. 2, lanes 3-5 and 7). When a SalI-
NotI fragment from the plasmid pMBO1367, encompassing around 14 kb upstream of the NotI site on asp, was
used as a probe, we observed the same 6.5-kb transcript plus one 3.8-kb transcript in embryonic mRNA corresponding to tld (Shimell et al., 1991 asp Encodes a Highly Basic Protein with Putative Actin
and Calmodulin Binding Domains
The 6.5-kb asp cDNA encodes a predicted polypeptide of
1,863-amino acid residues that has no homologues in the
GenBank/EMBL/DDBJ database (Fig. 3 A). The asp protein is predominantly hydrophilic and strikingly basic, having a calculated pI of 10.8. Its secondary structure is predicted to be mostly
The asp1 Allele Encodes a Truncated or Internally
Deleted Protein
To further characterize the asp gene product, we raised
rabbit polyclonal antibodies to a truncated Asp protein expressed in E. coli. The NcoI-BamHI fragment of cDNA 6a
(Fig. 4) was subcloned into the expression vector pET23d.
The resulting construct, pASP36, expresses a polypeptide
corresponding to amino acid residues 1-512, with a predicted molecular weight of 55.9 kD. This polypeptide does
not contain the putative actin binding domain of the Asp protein.
The resulting serum Rb3133 recognizes one polypeptide
of ~220 kD in immunoblots of third instar larval brains
(Fig. 5). To confirm that Rb3133 reacts specifically with
the Asp protein, brains from larvae of the transgenic strain
1366 (see Materials and Methods) were used in Western
blot analysis (Fig. 5, lane 2). Flies transformed with
pMBO1366 are expected to synthesize two forms of the
Asp protein: full length protein of 220 kD, derived from
the endogenous copy of asp at 96A and a truncated form
derived from the transgene. As expected, the serum detects the 220-kD wild-type asp protein plus a 124-kD polypeptide corresponding to the COOH-terminal truncated
form of the asp protein encoded by plasmid pMBO1366.
When extracts from brains of asp1 homozygous larvae
were analyzed (Fig. 5, lane 3), the 220-kD protein is no
longer observed, but the serum labels a polypeptide of
~130 kD. As the polyclonal antibodies were raised against
the NH2 terminus of the Asp protein, it seems that the asp1
mutation results in a COOH terminus truncation or in an
internal deletion of the Asp protein.
The Asp Protein Associates with Microtubules
As mutations in asp affect the behavior of spindle microtubules, we sought to determine whether the Asp protein
was itself microtubule associated. We purified microtubules from Drosophila embryos and took aliquots at each
stage of the purification for electrophoresis and blotting
onto PVDF membranes. These blots were incubated with
the polyclonal serum Rb3133 and with the monoclonal antibody Bx69, which detect the Asp protein and
We further assessed the ability of Asp protein to bind
microtubules in overlay assays in which bacterially expressed Asp fragments were separated by SDS-PAGE and
blotted to a membrane that was subsequently incubated
with microtubules. Bound microtubules were then detected by probing with the monoclonal anti-
The Asp Protein Associates with the Mitotic Spindle
Mutations in asp affect the morphology of both the mitotic
spindle at several developmental stages and the meiotic
spindle. At all stages mutant spindle microtubules may be
described as having a long and wavy appearance, and it is
not uncommon to see the loss of bipolarity in the form of
hemispindle structures. To determine whether Asp protein is a constituent of the wild-type spindle we used the
Rb3133 antibody to localize the Asp protein with respect
to microtubules in mitosis in syncytial embryos (Fig. 8).
During interphase, Asp protein appears to be distributed
throughout the cytoplasm (Fig. 8 a), but as the syncytium
enters mitosis and the bipolar spindle is formed, Asp is
seen in association with the polar regions (Fig. 8 b). This
polar association becomes tighter throughout metaphase
and anaphase (Fig. 8, c and d), but at telophase, as the
chromatin is decondensing and the spindle begins to disassemble, the Asp protein appears to move away from the
region occupied by the centrosome onto the central region of the spindle microtubules.
Several pieces of evidence confirm the molecular identity
of the asp gene. The full length gene rescues asp in germline transformants, whereas a truncated gene does not.
Furthermore, antibodies raised against a segment of the
gene expressed in E. coli recognize a truncated form of Asp
protein in asp1 homozygotes. These same antibodies have
allowed us to examine the subcellular distribution of the
Asp protein in cytological preparations by immunostaining and biochemically after subcellular fractionation. These
experiments show the Asp protein to copurify with microtubules after Taxol-induced tubulin polymerization and that it remains associated with the microtubules in salt
concentrations that remove many other MAPs. That the
Asp protein is a MAP is confirmed by its association with
the polar regions of spindles in mitosis.
Asp is the first metazoan MAP to be identified through
a genetic approach, and it is gratifying that the naming of
the gene by Ripoll et al. (1985) It is instructive to compare the domain organization and
microtubule binding properties of Asp with other MAPs
that have been characterized. These are principally of mammalian origin, and most have been purified from brain as
proteins associated with the stable microtubules of the axons and dendrites of neurons. In some cases, these proteins may be expressed specifically in brain. However, in
many cases their presence in other tissues is being confirmed. MAP2, for example, is predominantly found in brain
but has recently been shown to be present in rat testes (Loveland et al., 1996 It may be not only that microtubule dynamics are affected by the asp mutation but possible that the function
of other MAPs, in particular motor molecules, could be
impaired if the mutant protein can still bind microtubules.
The binding of MAP2, for example, is known to inhibit the
motility of kinesin and cytoplasmic dynein, but this is reversed by phosphorylation of the protein (Lopez and
Sheetz, 1995 The presence of wild-type Asp protein in the polar regions of the spindle points to this being the site of its action and suggests that it may play a crucial role in regulating the dramatic changes of microtubule dynamics upon
the entry into mitosis. Its presence in the astral microtubules may be important to mediate interactions between
microtubules and the actin cytoskeleton. This function is
suggested by the actin-binding motif in the central domain
of the protein. This motif is homologous to ones seen in a
number of actin bundling or gelation factors. These include The presence of a potential calmodulin binding site in
the central region of the molecule implies that the Asp
protein may be responsive to fluxes in calcium ions known
to occur in the polar regions of the spindle. Preliminary experiments show that the motif present in Asp enables it to
bind to calmodulin in a Ca2+-dependent manner (data not
shown). Calmodulin is a member of the EF hand superfamily of proteins. There is strong evidence for a direct
role for two members of this family in spindle pole body
(SPB) function in budding yeast. Calmodulin itself has
been shown by both genetic and molecular studies (Geiser
et al., 1993 It is evident that many questions are thrown open by our
finding that the Asp protein is a MAP containing potential
actin and calmodulin binding motifs. However, our work
has now generated the molecular tools with which we can
begin to address some of the above issues.
). The frequency
and size of such clones correlate inversely with the amount
of maternally contributed asp+ gene product.
). Several mitotic abnormalities may be observed in third instar larval neuroblasts
from asp homozygotes. There is an increased mitotic index
and a high frequency of polyploid cells. Many metaphase
figures have very highly condensed chromosomes, suggestive of a delay in progress through metaphase, and anaphase figures appear abnormally broad. asp1 animals show
reduced fertility, and a high frequency of nondisjunction has been observed in both divisions of male meiosis. Phase
contrast microscopic analysis of meiosis in homozygous
asp males revealed the abnormalities of spindle structure
that gave the locus its name (Ripoll et al., 1985
) and that
were subsequently confirmed by electron (Casal et al.,
1990
) and immunofluorescent microscopy (Gonzalez et al.,
1990
). Similar spindle abnormalities were seen in mitosis
in the larval neuroblasts of strong asp hypomorphs (Gonzalez et al., 1990
). Typically these mitotic cells have long wavy arrays of microtubules. Hemi-spindles are frequently
observed in which a long dense array of microtubules is
nucleated from a single centrosome.
). However, survivors are female sterile and produce syncytial embryos displaying a
number of abnormalities in nuclear divisions. One class of
these embryos has no DNA, as judged by fluorescent staining, presumably reflecting failure of germline mitosis. A
second class shows a variety of problems in nuclear division, including an abnormal ratio of centrosomes to nuclei and broadened or fused spindles with wavy microtubules.
Materials and Methods
; Saunders et al., 1989
), in the lambda insertion vector NM1149 (Murray, 1983
). The recombinant clones were screened
with labeled genomic DNA to identify clones containing repetitive DNA.
Clones that appeared to be single-copy DNA were mapped by in situ hybridization to verify that they were derived from the asp region. A second
chromosome microdissection was carried out using PCR amplification, as
described previously (Saunders et al., 1989
). In this case, two serial sections were performed through the 96A21-96B10 region of one chromosome. The amplifications yielded pools of DNA fragments of mean size
300 bp, as expected for Sau3A digestion. Microclone inserts and pools of
DNA were labeled by random oligonucleotide priming and used to screen
the cosmid libraries.
) or Lorist6 (Siden-Kiamos et
al., 1990). The initial screening was carried out with cloned DNA arising
from the first microcloning experiment. Subsequent steps of the walk used
terminal RNA probes synthesized from the promoters at the ends of the
cloning vectors. Periodically, the progress of the walk was monitored by in
situ hybridization as described (Saunders et al., 1989
). In situ hybridization of fragments from the chromosome walk to In(3R+3L)ats chromosomes (with which the deficiency-bearing In(3R)Ubx7LLatsR was synthesized; Gonzalez et al., 1989
) enabled the location of the distal breakpoint
of the deficiency In(3R)Ubx7LLatsR to be determined. To map the distal
breakpoint of Df(3R)Hd
1, in situ hybridization with fluorescent labels
(Saunders, 1994
) was used to visualize signals derived from one or both
homologues of 3R.
ZAP and also embryonic cDNA libraries (provided by Brown, N.;
Brown and Kafatos, 1988
).
). Plasmid pMBO1367 contains an 18-kb SalI
fragment that includes both the tld and asp transcripts; pMBO1366 contains a 14-kb SalI fragment that includes the tld transcript but only the 5
half of the asp transcript. Females with the genotype +/+; asp1 red/TM6B
were crossed to 1367/1367; +/+ males. 1367/+; asp1 red/+ males were selected from the F1 progeny and crossed to +/ +; asp1 red/TM6B females
to obtain asp1 red/asp1 red individuals. 1367; asp1/asp1 animals did not
show any morphological defect and were fully fertile, indicating that the
1367 insertion rescues the asp1 homozygous lethal phenotype. As a control, the same crosses were carried out using 1366 transformants. This insertion failed to rescue the asp1 mutation.
). Template clones for sequencing
were produced by exonuclease III deletion (Sambrook et al., 1989
) of inserts subcloned in Bluescript SK+ and KS+. Sequences were assembled
using Microgenie (Beckmann Instruments, Inc., Fullerton, CA) and Lasergene (DNASTAR Inc., Madison, WI) software. Searches of GenBank/
EMBL/DDBJ database were conducted using BLAST with the MRC human genome mapping project computer. The protein sequence was analyzed using DOMAIN, SAPS, and COILS programs.
. Polyclonal sera
were prepared by injecting rabbits as described (Harlow and Lane, 1988
).
Fig. 2.
Developmental pattern of expression of asp and
its flanking transcripts. Northern blot analysis of asp expression (asp transcript is
~6.5 kb). An Nco1-EcoRI
fragment from the 6.5-kb asp
cDNA clone was used as the
probe. Poly A+ is from 0-3
h-old embryos (lane 1), 3-6
h-old embryos (lane 2), first
instar larvae (lane 3), second instar larvae (lane 4),
third instar larvae (lane 5),
adult females (lane 6), and
adult males (lane 7).
[View Larger Version of this Image (47K GIF file)]
. Propidium iodide was used to visualize DNA; the rat monoclonal
antibody YL1/2 (Kilmartin et al., 1982
) was used to stain microtubules,
and the rabbit polyclonal antibody Rb3133 was used to stain the Asp protein. Staining was revealed using Texas red-conjugated goat anti-rat antibody and fluorescein-conjugated goat anti-rabbit antibody. All commercial antibodies were obtained from Jackson ImmunoResearch Labs, Inc.
(West Grove, PA). Images were collected using an Optiphot microscope
(Nikon Inc., Garden City, NY) with a confocal scanning head (model 600;
BioRad, Richmond, CA) and merged using Adobe Photoshop V2.5.
. Embryos were collected,
dechorionated in 50% bleach, and washed with tap water and then with
lysis buffer. About 3 ml of embryos were homogenized in 2 vol of ice cold
lysis buffer (0.1 M Pipes/NaOH, pH 6.6, 5 mM EGTA, 1 mM MgSO4, 0.9 M
glycerol, 1 mM DTT, 1 mM PMSF, 1 mg/ml aprotinin, 1 mg/ml leupeptin,
and 1 mg/ml pepstatin) with a Dounce homogenizer. The microtubules
were depolymerized by incubation on ice for 15 min, and the extract was
then centrifuged at 16,000 g for 30 min at 4°C. The supernatant was recentrifuged at 135,000 g for 90 min at 4°C. Microtubules in this latter supernatant were polymerized by addition of GTP, to a final concentration of 1 mM,
addition of taxol, to a final concentration of 20 mM, and incubation at
room temperature for 30 min. 3-ml aliquots of extract was layered on top of
3-ml 15% sucrose cushions prepared in lysis buffer supplemented with 20 mM taxol and 1 mM GTP. After centrifuging at 54,000 g for 30 min at
20°C using a swing out rotor, the pellet was resuspended in lysis buffer
containing taxol and GTP. To extract the MAPs, the concentration of
NaCl was adjusted to 0.4 M, and the samples were incubated for 30 min at
37°C. Microtubules were pelleted by centrifugation at top speed on a microcentrifuge for 30 min; the supernatant is considered to be the MAP
fraction.
Results
)
mapped asp to the cytological interval 96A21-96B10 between the distal breakpoint of In(3R)Ubx7LLatsR and the
breakpoint of T(Y;3)B197 (Fig. 1). At the onset of this work
there were no obvious molecular entry points into this region, and so we chose the technique of chromosome microdissection and microcloning to achieve this end. Two chromosomal segments corresponding to the asp interval were
microdissected and DNA fragments extracted for cloning
in the bacteriophage
insertion vector
1149. The chromosomal origins of these clones were confirmed by in situ hybridization, before using them to screen phage
and
cosmid genomic libraries (see Materials and Methods).
A resulting chromosome walk of 130 kb was carried out
and clones correlated with the polytene chromosome
map by in situ hybridization. The distal breakpoint of
In(3R)Ubx7LLatsR, which does not uncover asp and defines
the proximal boundary of the region known to contain asp,
was mapped to a 5.8-kb BamHI fragment at the extreme
proximal end of the walk. The distal end of the chromosome walk maps to the interband between 96A21-25 and
96B1-10. Subsequently, we found that a cytologically invisible deficiency Df(3R)Hd
1 uncovers not only the nearby
zygotic embryonic lethal tolloid (tld), as reported by
Shimell et al. (1991)
, but also asp.
Fig. 1.
A cytogenetic and
molecular map of the asp region. A is a representation of
divisions 95 and 96 of the
polytene map of chromosome arm 3R. Subdivisions
A and B are indicated. The
rearrangements used by
Gonzalez et al. (1989) to define the asp region, In(3R)Ubx7LLatsR and Df(3R)A117PB197D are indicated
above the chromosomes. B
shows the proximal section of the chromosome walk
linked to the cytological map.
B, R, and S represent BamHI,
EcoRI, and SalI restriction
sites, respectively. Horizontal lines below the restriction
map indicate phage lambda and cosmid clones isolated
from genomic libraries. Boxed
fragments indicate fragments
mapped by in situ hybridization. One contains the distal
breakpoint of In(3R)Ubx7LLatsR (ats), while the other is
an EcoRI fragment recovered by microcloning. C is an
expansion of the proximal
section of the chromosome
walk. The distal breakpoints
of In(3R)Ubx7LLatsR and
Df(3R)Hd
1 are indicated.
Uncertainties about the cytological limits of the deficiencies are indicated by the thinner lines. The limits of the
deletion associated with the
P element insertion tld allele tld68-62 are indicated by the
bar. D illustrates the transcription units of tld and asp
as well as the two fragments
used in P element mediated rescue experiments. pMBO1367 contains an 18-kb SalI fragment containing both tld and asp. pMBO1366
contains a 14-kb SalI fragment containing tld but only the 5
portion of asp.
[View Larger Version of this Image (30K GIF file)]
). This yielded 6.5-kb cDNA
clones corresponding to a transcription unit lying immediately distal to tld (Shimell et al., 1991
). The tld68-62 mutation results from a P element insertion associated with a deletion of ~4 kb that removes the entire tld transcription
unit and ~1 kb of the 5
end of the gene encoding the 6.5kb transcript. Shimell et al. (1991)
showed that tld can be
rescued by two constructs, pMBO1366 and pMBO1367.
Although pMBO1366 rescues the early embryonic zygotic
lethality of tld68-62, the rescued flies exhibit cuticular defects. As such defects are associated with asp, it seemed
that tld68-62 could be a tld asp double mutant. Consequently we tested whether these same transformants would
rescue the recessive lethality of asp1. pMBO1366 contains
tld but only the 5
end of the candidate asp gene, whereas
pMBO1367 contains both transcription units (see Materials and Methods; Fig. 1). We found that the pMBO1367
rescues the lethality of asp1 mutants while pMBO1366
does not. Taken together, these data confirm the identity
of the 6.5-kb transcription unit as asp.
) and a 5.2-kb transcript presumably corresponding to the recently described
tolkin gene (Finelli et al., 1995
; and data not shown).
-helical. Analysis of the protein using
the COILS program shows that short stretches of amino
acids near the COOH terminus have the potential to form a coiled coil. There is a small sequence lying between residues 848 and 870 that has significant similarity to the core
actin binding domain of a number of actin binding proteins, such as
-actinin (Noegel et al., 1987
; Blanchard et
al., 1989
), fimbrin, spectrin, dystrophin (de Arruda et al.,
1990; Matsudaira, 1991
), and the Dictyostelium discoideum
ABP120 (Bresnick et al. 1990
; Fig. 3 B). These proteins either bundle actin filaments together (for example
-actinin) or attach actin filaments to other cellular structures.
A second sequence lying between residues 938 and 968 corresponds to the conserved calmodulin binding (IQ)
motif (Cheney and Mooseker, 1992
) present in neuromodulin, a neuron-specific membrane-associated protein (Chapman et al., 1991
); neurogranin, a neuron specific protein
kinase C substrate (Baudier et al., 1991
); the igloo gene
product, a calmodulin-binding protein from the Drosophila central nervous system (Neel and Young, 1994
); and in
the "neck" regions of most forms of conventional and nonconventional myosin (for review see Cheney and Mooseker,
1992
; Fig. 3 C). In addition Asp shows six consensus sites
for phosphorylation by p34cdc2 and four consensus sites for
phosphorylation by MAP kinase. Interestingly, these are
all clustered in the NH2-terminal third of the molecule.
Fig. 3.
(A) The sequence of the Asp protein. Consensus sites
for phosphorylation by p34cdc2 and MAP kinases are shown. Also
shown are the putative actin and calmodulin binding sites. (B)
Comparison of actin binding motifs. Comparison between Asp
putative actin binding site and actin binding sites present in -actinin, spectrin, plastin, ABP120, dystrophin, and fimbrin. Only a selection of these actin binding domains is shown. Identical residues to Asp are shaded. (C) Comparison of calmodulin binding
motifs. A comparison between the Asp putative calmodulin binding site and the calmodulin binding sites present in a number of
other proteins. This list is not comprehensive. Identical residues
have been shaded.
[View Larger Versions of these Images (64 + 35K GIF file)]
Fig. 4.
Asp expression constructs. The upper portion of the
figure shows the asp cDNA indicating restriction cleavage sites
and localization of the putative actin and calmodulin binding
sites. Segments of the protein expressed in E. coli are indicated
by shaded bars. The truncated protein expressed in flies transformed by pMBO1366 is indicated by a solid line.
[View Larger Version of this Image (15K GIF file)]
Fig. 5.
Shortened proteins are produced by the asp1 mutant
and by pMBO1366 transformants. Western blot analysis of asp
expression using the antibody Rb3133. (Lane 1) Proteins from 8 wild-type larval brains; (lane 2) proteins from 8 brains of larvae
transformed by pMBO1366; (lane 3) proteins from 12 asp1/asp1
larval brains.
[View Larger Version of this Image (44K GIF file)]
-tubulin,
respectively (Fig. 6, A and B). Enrichment in tubulin is
paralleled by an enrichment in the Asp protein. Moreover,
the Asp protein seems to bind microtubules with a very
high affinity as it is not released from the microtubule pellet by salt conditions that are known to dissociate most
MAPs (Fig. 6, lanes 6). Asp protein is also not liberated after incubation with ATP (data not shown).
Fig. 6.
Asp copurifies with microtubules. A shows a Western
blot fractionated by 7.5% SDS-PAGE. B shows the same protein
preparations on a Coomassie blue-stained 10% SDS-PAGE. (A
and B) Microtubule purification from 0-3-h-old Drosophila embryos after Taxol-induced polymerization. Asp was detected using the Rb3133 antibody and tubulin by the Bx69 antibody. Samples are as follows: (lane 1) 20 µg of crude embryonic protein
extract; (lane 2) 20 µg of pellet after the 16,000 g centrifugation;
(lane 3) 20 µg of protein from the supernatant fraction after sucrose gradient centrifugation; (lane 4) 10 µg of the microtubules
and associated proteins; (lane 5) 5 µg of the final microtubule
preparation; (lane 6) 10 µg of the final MAP preparation.
[View Larger Version of this Image (33K GIF file)]
-tubulin antibody Bx69. These assays were performed using either total E. coli proteins from cells expressing Asp11 and Asp36
(Fig. 7, A and B) or the corresponding purified fusion proteins (Fig. 7 C). Microtubules were observed to bind to
both Asp36 and Asp11, but binding to Asp36 appears to be
of a higher affinity (Fig. 7, B and C). Binding seems to be
specific, as no detectable binding of microtubules to total
E. coli proteins was observed in strains that had not been
induced to express the fusion proteins (Fig. 7, A and B). To ensure that differences in binding did not simply reflect
differences in the degree of induction of the two fusion
proteins in the bacteria, we repeated the microtubule
binding assay using increasing amounts of the two purified
fusion proteins (Fig. 7 C). Differences in the binding affinity can be seen at all protein concentrations; Asp11 binding to microtubules was barely detectable when 1.6 µg of
protein are used, whereas microtubule binding to Asp36 is
readily detectable when 0.4 µg of protein were blotted to
the membrane. This shows that the NH2-terminal third of
the Asp protein binds more avidly to microtubules than
does the central part of the molecule.
Fig. 7.
The NH2-terminal
segment of Asp binds polymerized tubulin in vitro. (A)
SDS-PAGE of total proteins
from noninduced E. coli strain carrying pAsp11 (lane
1), E. coli pAsp 11 induced to
express (lane 2), and E. coli
pAsp36 induced to express
(lane 3). An overlay of such a
gel is shown in B, in which
the blotted proteins have been overlaid with polymerized tubulin that is subsequently detected with the
Bx69 monoclonal anti--
tubulin (see Materials and
Methods). C shows an overlay assay carried out on the
indicated amounts of purified Asp segment after expression in E. coli.
[View Larger Version of this Image (25K GIF file)]
Fig. 8.
Immunolocalization of Asp in the mitotic cycle in syncytial embryos. Simultaneous staining for DNA with propidium iodide
and tubulin with YL1/2 primary antibody and rhodamine-conjugated goat anti-rat IgG are shown in the first column and subsequent red channel of the merged image. Staining of Asp using Rb3133 primary antibody and FITC-conjugated goat anti-rabbit IgG is shown in the
middle column and subsequent green channel in the merged image. The mitotic phases are (a) interphase, (b) prophase, (c) metaphase, (d) anaphase, and (e) telophase. The scale bar refers to the main set of panels. Single mitotic figures have been selected for the inset at a
fourfold greater magnification. Bar, 25 µm.
[View Larger Version of this Image (112K GIF file)]
Discussion
was predictive of the localization and function of the protein. The protein is highly
basic, and in its central region lies a putative actin-binding
motif. Basic regions within other MAPs play a significant
part in conferring the ability to bind microtubules. The
Asp protein is no exception to this rule in that at least one
segment of this basic molecule, a polypeptide comprised of
the 512 NH2-terminal amino acids, can be shown to bind
microtubules in vitro. The central region of the protein binds microtubules poorly. Unfortunately, we have been
unable to test the microtubule binding ability of the
COOH-terminal region, as this segment of the protein appears unstable in both bacterial and insect cell expression
systems.
). In general, MAPs bind to microtubules to increase their stability. Studies of MAP2C in vivo,
for example, show that it bundles and stabilizes microtubules in cultured fibroblasts (Umeyama et al., 1993
). Moreover, in vitro studies show that MAP2 can decrease the
frequency of transitions between the growth and shortening of microtubules leading to net microtubule growth
(Kowalski and Williams, 1993
; Itoh and Hotani, 1994
).
Like several other MAPs, MAP2 has a short "promoter"
domain, which binds to microtubules to promote assembly, and a "projection" domain thought to interact with
other cytoskeletal elements. Fellous et al. (1994)
have digested MAP2 with chymotrypsin to remove the projection
domain and find that the residual promoter domain differs
from the full length protein in that it binds microtubules to
organize them into clusters of spirals. Moreover, assembly
of microtubules in the presence of the promoter domain
alone is more resistant to certain depolymerizing drugs
than assembly in the presence of the full length protein. Thus the projection domain would appear to modify the
interactions of MAP2 with microtubules in their assembly.
The Asp protein may be seen as being structurally similar
and functionally analogous, having an NH2-terminal microtubule binding domain and central and COOH-terminal regions with the potential to interact with other cytoskeletal components.
). This specifically requires a cAMP-dependent kinase that copurifies with MAP2, and it is not affected by phosphorylation with p34cdc2 kinase, mitogen activated protein kinase, or the NIMA mitotic kinase. In
contrast, phosphorylation of MAP4 by p34cdc2 kinase abolishes its microtubule stabilizing activity, interestingly without altering its ability to bind microtubules (Ookata et al., 1995
). MAP4 is the major MAP of many mammalian cell
lines and is present along the length of both interphase
and mitotic microtubules (Bulinski and Borisy, 1980
; DeBrabander et al., 1981). There is evidence that MAP4
mediates the association of cyclin B with microtubules in
both the starfish oocyte and in cultured mammalian cells
(Ookata et al., 1992
, 1993
, 1995
). It may therefore provide
a general mechanism for destabilizing interphase microtubules upon entry into mitosis. Other MAPs could also
have such a role, and as with the kinesin-like proteins,
MAPs could be functionally redundant. A 230-kD protein,
XMAP 230, has been purified from Xenopus oocytes and
found to be present in all dividing cells (Andersen et al., 1995
). It is localized to microtubules in interphase but dissociates from them upon entry into mitosis, to reassociate
later in the mitotic cycle. It is phosphorylated by mitotic
extracts whereupon it has a reduced affinity for microtubules. Upon binding it increases microtubule growth rate,
decreases the rate of shrinking, and suppresses microtubule loss by "catastrophe." It thus stabilizes interphase microtubules and can locally modify microtubule behavior during mitosis. As the Asp protein has numerous sites for
phosphorylation by p34cdc2 and mitogen-activated protein
kinase, it is not inconceivable that cyclical phosphorylation
could affect its properties throughout the mitotic cycle.
-actinin (Noegel et al., 1987
; Blanchard et al., 1989
), a filamentous actin cross-linking protein found in stress fibers
and adhesion plaques in nonmuscle cells and in Z-discs in
muscle cells; it has a single actin binding motif and can
cross link actin filaments into a homodimer. Single actin
binding motifs are also found on dystrophin and spectrin
(de Arruda et al., 1990; Matsudaira, 1991
). Fimbrin, an actin-associated protein found in microvilli and filopodia, on
the other hand, has a duplicated actin binding motif that
enables it to bundle actin filaments as a monomer (de Arruda et al., 1990). It has homology to plastins, cytoplasmic
actin binding proteins, and the protein encoded by the
yeast gene ABP7 or Sac6p (Adams et al., 1995
). The association of microtubule and microfilament networks is nowhere more obvious than in the vicinity of centrosomally nucleated asters. In the syncytial blastoderm Drosophila
embryo, for example, the actin caps that form above interphase nuclei are positioned by the centrosome and its associated microtubules. Moreover, the spatial coordination
of mitosis and cytokinesis requires the contractile actin
ring that will dictate the cleavage furrow to be correctly
positioned with respect to the spindle. Classical experiments with echinoderm eggs showed that moving the spindle by micromanipulation resulted in the corresponding repositioning of the cleavage furrow. However, the experimental manipulation of such embryos to produce two mitotic spindles within a single cell led to the generation of a
third cleavage furrow between the asters of the juxtaposed
spindles (for review see Rappaport, 1986a
,b). The nature
of the molecular "signal" that positions the actin ring with
respect to the asters is unknown, but it is possible that a
molecule such as the Asp protein could fulfill this role.
; Stirling et al., 1994
, 1996; Sundberg et al., 1996
)
to be essential for spindle function as a result of its interaction with the COOH terminus of Spc110p, an essential
protein with a large coiled-coil domain that provides a
spacer between the central and inner plaques of the SPB
(Kilmartin, 1993). The second EF hand protein localized to the SPB is the product of the CDC31 gene, which associates with the KAR1 gene product to form a complex
(Spang et al., 1993
, 1995
; Biggens and Rose, 1994; Vallen
et al., 1994
). Roles for these proteins in spindle function
are likely to be conserved. Calmodulin is the major intracellular Ca2+ receptor and is involved in many cellular
processes; it is known to be localized to the microtubules
of metazoan spindles (e.g., Stemple et al., 1988
), and it appears to be important in regulating the function of its
dependent kinase and phosphatase in the metaphase- anaphase transition and in spindle behavior (Morin et al.,
1994
; Yoshida et al., 1994
). CDC31 is the yeast homologue
of centrin, a protein first identified in the Chlamydomonas
basal body and subsequently found to be a universal component of centrosomes in animal cells (for review see Salisbury, 1995
). There are therefore a large number of potential
ways in which Asp protein function might be mediated
through an association with one of these EF hand proteins.
Received for publication 5 October 1996 and in revised form 30 January 1997.
1. Abbreviations used in this paper: asp, abnormal spindle; spb, spindle pole body; tld, tolloid.We would like to thank Fiona Cullen for sequencing; Mar Carmena and Daryl Henderson for their comments on the manuscript; Alvaro Tavares for his suggestions and help; and Dr. M. O'Connor for supplying lines transformed with pMBO1366 and pMBO1367.
This work was supported by research grants from Science and Engineering Research Council, Medical Research Council, Cancer Research Campaign, and the Human Capital and Mobility Programme of the European Union. Thomas Howard is grateful for a studentship from BBSRC and Maria do Carmo Avides for an European Molecular Biology Laboratory Long-Term Fellowship.