Article |
2 Division of Biological Sciences, University of Missouri, Columbia, MO 65211
3 Institute of Biological Chemistry, Washington State University, Pullman, WA 99164
4 Plant Science Institute, Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
5 Graduate School of Bioagricultural Science, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan
Address correspondence to John C. Rogers, Institute of Biological Chemistry, Washington State University, Pullman WA 99164-6340. Tel.: (509) 335-2773. Fax: (509) 335-7643. E-mail: bcjroger{at}wsu.edu
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Abstract |
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Key Words: storage protein; prevacuolar compartment; lytic vacuole; multivesicular body
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Introduction |
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However, recently we demonstrated that the matrix is a destination of soluble proteins, but the PSV crystalloid additionally contains integral membrane proteins and substantial amounts of lipid (Jiang et al., 2000). This membrane-containing internal structure results from uptake of cytoplasmic prevacuolar organelles, termed dark intrinsic protein (DIP) organelles (Jiang et al., 2000), that are likely to be equivalent to precursor-accumulating (PAC) vesicles characterized from developing pumpkin and castor bean seeds (Hara-Nishimura et al., 1998). These prevacuolar organelles receive protein traffic directly from the ER and via the Golgi complex (Hara-Nishimura et al., 1998; Jiang et al., 2000). In addition to the condensed appearing osmiophilic contents that assemble into the PSV crystalloid, the prevacuolar organelles also contain internal vesicles (Hara-Nishimura et al., 1998; Jiang et al., 2000). In PAC vesicles, indirect evidence indicates that the internal vesicles may contain proteins trafficking from the Golgi complex (Hara-Nishimura et al., 1998). Thus, uptake of the organelles into the PSV would also result in uptake of internal vesicles that are likely to have a fate separate from components assembled into the crystalloid.
Here, we demonstrate that the globoid cavity is defined by a unit membrane. This membrane is specifically marked by the presence of vacuolar H+-pyrophosphatase (V-PPase) and -TIP. In transgenic plants expressing a chimeric integral membrane reporter protein (designated Re-F-B-B) that traffics to the lytic prevacuolar compartment where it is processed proteolytically (Jiang and Rogers, 1998), the globoid cavity contains the processed form of the reporter protein. Additionally, as assessed in wild-type plants the globoid cavity contains a tobacco protein closely related to the barley cysteine protease aleurain. Thus, it has integral membrane and soluble markers characteristic of an LV.
In most plant cells, as far as is known, LVs and vacuoles that have characteristics of PSVs are separate structures (Di Sansebastiano et al., 1998, 2001), and trafficking pathways to them utilize prevacuolar compartments that also are distinct (Jiang and Rogers, 1998). However, in specific cells where the complex type of PSV formation as described here occurs, predominantly in the seed embryo but also in certain root tip cells, Re-F-B-B traffics through the cytoplasmic prevacuolar DIP organelles/PAC vesicles where it is also present in proteolytically processed form; these same organelles also contain membrane proteins that are destined for the PSV crystalloid. This result indicates that proteins within the prevacuolar organelles destined for the PSV globoid are partitioned away from proteins destined for the PSV storage compartment and provides the basis for a model by which a globoid compartment and its contents could be assembled within the developing PSV.
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Results |
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In PSVs from tissue treated with 0.5% permanganate, the globoid crystals had been removed by permanganate treatment, and globoid cavities were identified readily by their content of numerous wisps of flocculent-appearing material that were not present in the lipid spaces surrounding the PSVs (Fig. 1, A and B, insets). These cavities were defined by an electron-dense border, which when cut perpendicularly demonstrated the appearance typical of a unit membrane (Fig. 1, A and B, arrows). Permanganate fixation is known to result in a granular appearance on membranes; this granularity is evident both on the globoid membranes and the PSV tonoplast (Fig. 1 C). To exclude the possibility that the unit membrane appearance shown in Fig. 1, A and B, was due to an artifact of stain deposited on both sides of the thin sections, sections of tissue treated with 2.5% permanganate were viewed without additional staining. Thus, any electron density of structures would result from labeling by permanganate and bismuth exposure before the tissue was embedded. Treatment with the higher permanganate concentration resulted in heavy labeling of the surface of oil bodies surrounding PSVs and loss of a visible PSV tonoplast; however, an electron-dense boundary around globoid cavities was still present (Fig. 1 D). When viewed at higher magnification, it is evident that this boundary retains a unit membrane appearance (Fig. 1 E, arrows). These results strongly support the concept that the globoid cavity is defined by a membrane.
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In PSVs in mature tomato embryos, the globoid is particularly prominent and can be identified readily as a large round structure protruding from one end of the PSV (Spitzer and Lott, 1980) (Fig. 2 A). Electron microscopic thin-section and freeze-fracture studies have demonstrated that the globoid is contained entirely within the PSV (Lott, 1980; Spitzer and Lott, 1980). When studied by wide-field immunofluorescence, the PSV tonoplast labeled for -TIP, whereas the globoids were outlined by labeling for V-PPase (Fig. 2, A and B). To determine how frequently V-PPase labeling coincided with structures having the appearance of globoids, we used confocal immunofluorescence and obtained multiple optical sections that were analyzed by superimposing pixels representing specific fluorescence on visible wavelength images that were collected concurrently with the fluorescent images. An example is presented in Fig. 2 C. On the right is a visible image where solid arrows indicate globoids that had fluorescent labeling in at least one optical section, and arrowheads indicate globoids that were not labeled. To the left is the same image with immunofluorescence pixels superimposed. In two collections of images comprising 4 and 6 optical sections, respectively, 90% of the globoids were labeled. Thus, most structures morphologically identified as globoids labeled with antibodies raised to the purified V-PPase protein. We then used a matrix marker antibody to define the PSV matrix and thereby outline internal spaces defined by globoid (Fig. 2 D, thick arrows) and crystalloid (Fig. 2 D, thin arrows); in double-labeling experiments, V-PPase labeling was clearly on structures within PSVs that were surrounded by matrix (Fig. 2 E). These experiments used both the antiserum raised to purified V-PPase (Fig. 2, AC) and the 1368 antipeptide antiserum (Fig. 2 E). To further test whether the immunofluorescence labeling observed was specific for V-PPase and not due to cross-reactivity with another protein, we compared labeling obtained with 1368 antiserum to that obtained with the two other antipeptide antisera, 1401 and 324; all antisera colocalized on structures with the appearance characteristic of globoids (Fig. 2, F and G, arrows). These results, where four different antisera each raised to a different set of V-PPase epitopes gave similar labeling patterns, strongly support the conclusion that V-PPase protein is in a membrane surrounding the globoid. The results also indicate that little V-PPase, as detected by the antibodies, is present in the PSV tonoplast in tomato embryos.
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Immunolocalization of -TIP to globoid cavity membrane
We sought to characterize the globoid cavity membrane further by identifying other proteins that were localized to it. As noted above, a protein of the appropriate size for a TIP was recognized by our anti-TIP peptide polyclonal antibodies in globoid preparations from tomato seeds. We tested further the specificity of this association with confocal immunofluorescence studies using three different anti
-TIP antibody preparations on sections of embryos from seeds from three different plant species, tobacco, tomato, and snapdragon (Antirrhinum majus). Results from tobacco are shown in Fig. 4 where the anti
-TIP peptide monoclonal antibody MAb351 was used. In Fig. 4 A, the different symbols point to structures within PSVs having a morphology consistent with globoids (Fig. 3 A, top, left). When labeling for V-PPase (red) was compared with that for
-TIP (green), both antibodies colocalized on these globoid-like structures as shown in the merged image.
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When labeling on sections of embryos from tomato and snapdragon obtained with antiserum raised to purified -TIP, VM23 (Maeshima, 1992; Higuchi et al., 1998) was compared with labeling obtained with antiserum to purified V-PPase protein (Fig. 5 A), V-PPase (red), and
-TIP (green) colocalized on structures within PSVs as demonstrated in the merged image (right). Similar results were obtained using the anti
-TIP peptide polyclonal antibodies, although they additionally gave weak tonoplast labeling (unpublished data). Consistent with their location on the globoid membrane, labeling for each protein was separate from labeling with anti-DIP antibodies (Fig. 5 B; DIP is a marker for the PSV crystalloid) (Jiang et al., 2000). The fact that three independently derived antibody preparations gave the same result argues strongly that the antibodies detected
-TIP rather than cross-reacting epitopes on other proteins. The fact that the polyclonal anti
-TIP peptide antibodies also labeled tonoplast may indicate the presence of a small amount of
-TIP there or may result from cross-reactivity with other TIPs in tobacco.
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We identified transgenic plants expressing Re-F-B-B by immunoprecipitating 35S-labeled proteins sequentially with the original antialeurain antibodies and then with anti-FLAG antibodies from root extracts separated into soluble and membrane fractions (Jiang et al., 2000). This strategy allowed us to detect the 50-kD full-length integral membrane reporter protein separated from any cross-reactivity the antibodies might have with the
34 kD soluble endogenous tobacco aleurain ortholog (Rogers et al., 1997); results are presented in Fig. 6 C. Consistent with previous results (Jiang et al., 2000), neither extracts from wild-type plants (Fig. 6 C, lanes 1 and 2) nor from plants transformed with a construct expressing Escherichia coli ß-glucuronidase (lanes 3 and 4) yielded radioactive bands the size appropriate for the reporter protein (position indicated by arrow). In contrast, in root extracts from three Re-F-B-B gene transformants (649-1, -2, and -3; lanes 6, 8, and 10) and in an extract from a positive control plant expressing Re-F-B-
(650; lane 12) a
50-kD band was present predominantly in the membrane fraction of each. To confirm the identity of the immunoprecipitated protein, a similar band was immunoprecipitated from the membrane fraction of suspension culture protoplasts transiently expressing Re-F-B-B (construct 491; lane 14). To test for accumulation of the reporter protein in seeds, a filter carrying extracts from wild-type and 649-2 seeds was probed with the WSU antibody. An
30-kD band similar in size to aleurain in a barley leaf extract (lane 7) was detected in the 649-2 soluble fraction (lane 5, arrow) but not in the wild-type seed fractions. The presence of a faint
34-kD band (asterisk) of similar intensity in soluble fractions from both wild-type (lane 3) and 649-2 (lane 5) indicates that similar amounts of protein were loaded in each and that the presence of the
30-kD band in 649-2 represents aleurain processed from Re-F-B-B and accumulated in the transgenic seed.
We used confocal immunofluorescence to localize the aleurain-like protein in wild-type (nontransformed) seeds. As shown in Fig. 7 A, the antialeurain antibodies from rabbit C (red) colocalized with anti-TIP MAb351 (green) that served as the globoid marker. When the fluorescent images were superimposed upon a visible light image collected simultaneously in the blue channel (left), consistent with results shown in Fig. 4, they labeled globoids (arrows). In contrast, when the barley aleurain-specific antibodies from WSU antiserum were used on sections from wild-type seeds, essentially no labeling above background was obtained (Fig. 7 B, red), whereas MAb351 labeling of globoids (Fig. 7 B, green) was similar to that in Fig. 7 A. When seeds from transgenic plant 649-2 expressing the Re-F-B-B reporter protein were studied, strong labeling with the WSU antialeurain antibodies was obtained (Fig. 7 C, red), which colocalized with MAb351 (green) on globoids. The tissue samples used in labeling studies shown in Fig. 7, AC, were fixed, processed, and labeled with antibodies concurrently, and the images were collected during the same microscopy session using identical parameters for each. We conclude that the WSU antialeurain antibodies are specific for barley aleurain associated with the reporter protein expressed from the transgene and that both the endogenous tobacco aleurain-like protein and the reporter protein were associated specifically with globoids. The fact that labeling for aleurain was present in a ring similar to
-TIP labeling on the globoid membrane would be explained if formation of the phytic acid crystal within the globoid displaced soluble proteins to the periphery.
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Aleurain colocalizes with a marker for multivesicular body prevacuolar organelles
We searched for labeling with the two different antialeurain antibodies in developing seeds in an attempt to identify a prevacuolar compartment that contributed to globoid formation. We found that both labeling for the endogenous tobacco aleurain-like protein in wild-type seeds (Fig. 8 A, green) and for the aleurain epitopes associated with Re-F-B-B (Fig. 8 B, green) colocalized with the TIP DIP on small punctate structures in cells from seeds collected at 12 d after pollination. These structures were indistinguishable from prevacuolar organelles identified previously in developing seeds by their labeling for DIP (Jiang et al., 2000).
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Discussion |
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The absence of microscopic evidence was relative rather than absolute. Some studies using standard osmium fixation protocols, particularly in systems where developing PSVs were present, showed osmiophilic structures surrounding globoid cavities that would be consistent with the appearance of membranes (Jones and Price, 1970; Monma et al., 1992; Krasowsky and Owens, 1993). However, these studies did not show a unit membrane structure, and it was possible that the osmiophilic structures resulted from dense compression of proteins. Additionally, freeze-fracture studies of globoids in dicotyledonous plant seeds did not demonstrate a cleavage plane at the globoid PSV interface that would be expected if a membrane surrounded the phytic acid crystals (Lott, 1980; Spitzer and Lott, 1980). However, other studies using cereal grains did demonstrate such a cleavage plane (Buttrose, 1971; Swift and Buttrose, 1972; Fernandez and Staehelin, 1985), although it was noted that the appearance of the interface layer differed from that of other membranes in the cell (Fernandez and Staehelin, 1985).
The lipid composition of a globoid membrane could differ substantially from that of the PSV tonoplast. For example, in mammalian cells internal membranes in multivesicular body late endosomes contain abundant lysobisphosphatidic acid (Kobayashi et al., 1998). Additionally, the fact that these results were derived from dry seeds in which a potential globoid membrane would have been desiccated onto the surface of a crystal of inositol hexaphosphate raises the possibility that such a membrane might have different physical characteristics than a tonoplast or plasma membrane, characteristics that could affect freeze fracture and staining with osmium. The staining characteristics of such a membrane would be unpredictable, and it is clear that developmental changes in lipid composition can greatly affect staining after treatment with osmium. For example, during oogenesis in the fern Paesia plastid envelopes become progressively less distinct after aldehyde fixation and osmication, but reaction to permanganate is unchanged; this phenomenon is due to a specific change in plastid membrane lipids because mitochondrial envelopes remain unchanged throughout oogenesis (Bell, 1983).
Our results with permanganate fixation to demonstrate a unit membrane boundary between globoid and PSV matrix are relevant in this regard: extensive treatment with permanganate resulted in loss of visible PSV tonoplast but maintained the globoid membrane. Since permanganate is known to react specifically with membrane lipids, especially phospholipids (Ongun et al., 1968), this difference may have resulted from different lipid compositions and lipid/protein ratios within globoid versus tonoplast membranes.
There are solid precedents for internal membrane-bound compartments within vacuoles. The intravacuolar chambers that contain calcium oxalate crystals are clearly defined by unit membranes (Arnott and Pautard, 1970), and formation of the chambers is preceded by the presence of abundant membrane vesicles within the vacuole (Franceschi, 1984). Additionally, when anthocyanins are produced in sweet potato suspension culture cells they are deposited in intravacuolar pigmented globules. A soluble metalloprotease, VP24, which is proteolytically processed from a 96-kD precursor that has multiple transmembrane domains, accumulates to high levels in the globules (Xu et al., 2001). The COOH-terminal portion of the 96-kD precursor was also localized to the intravacuolar globules and not the vacuole tonoplast (Xu et al., 2000). Interestingly, as judged by neutral red accumulation the globules are acidic, whereas the surrounding central vacuole is less so or not at all (Nozue et al., 1993; Kubo et al., 1995). Thus the globules are likely to be surrounded by a membrane.
These intravacuolar compartments must exist for the same reasons separate membrane-bound organelles exist within the secretory pathway: they define a specialized environment that is required for certain functions without interference from ongoing processes in other organelles. In the case of the globoid cavity, we hypothesize that the internal environment is optimal for formation of phytic acid crystals. The facts that the globoid membrane contains a pyrophosphate-driven proton pump, V-PPase, and that phytic acid molecules are known to carry pyrophosphate groups (Loewus and Murthy, 2000) raises the interesting possibility that the association of the two in the globoid may have functional significance. However, interpretation of this association is complicated by the fact that the orientation of the V-PPase protein in the globoid membrane is not known. Additionally, our finding that the globoid membrane contains -TIP, which is a marker for LVs, and that a marker for the LV pathway, Re-F-B-B, traffics to it together argue that the globoid represents an internal LV-like compartment. The globoid membrane would thus separate the storage compartment outside from potentially deleterious lytic functions inside. Our results indicate that a tobacco aleurain-like protein, possibly the tobacco ortholog of aleurain, is normally present in the globoid. It will be helpful to learn if other digestive enzymes are also present. In future studies, it will be important to follow the fate of globoid cavities within PSVs early during seed germination to learn if the globoid contents gain access to the storage compartment and participate in their breakdown.
Our results demonstrate that a PSV is the equivalent of a large multivesicular body. How does the internal globoid cavity originate? One possibility is that the globoid cavity represents a preformed LV that is then taken up into the developing PSV by autophagy, perhaps during the process by which enlarging PSVs displace preexisting LVs during seed development (Hoh et al., 1995). Such a process should result in a double membrane around the internalized structure, but our micrographs demonstrated only a single unit membrane around the globoid, an observation that would argue against a process involving autophagy (Teter and Klionsky, 2000). However we cannot exclude the possibility that one of the double membranes could have been removed by a digestive process after internalization. Alternatively, our localization of two different reporter proteins in transgenic plants to what appear to be the same prevacuolar organelles provides the basis for hypothesizing a different mechanism (Jiang and Rogers, 2001). We demonstrated previously that the contents of multivesicular prevacuolar organelles are taken up by PSVs during PSV development and internal membrane-containing structures in the organelles then assemble into the PSV crystalloid (Jiang et al., 2000). We would postulate that the globoid compartment would be formed from a separate membrane-defined compartment within the prevacuolar organelles (Jiang and Rogers, 2001). Such a mechanism would be consistent with the observation in developing castor bean endosperm that phytic acid was present within small cytoplasmic structures having the appearance of multivesicular bodies that appeared to be transported to the PSV where the phytic acid was taken up and assembled into the globoid (Greenwood and Bewley, 1984). This alternative model is limited by lack of information as to how a multivesicular body prevacuolar organelle could incorporate lytic enzymes selectively into its internal vesicles, but that is essentially the same question posed by the presence of lytic enzymes inside the membrane-bounded globoid cavity.
A final point should be emphasized. These studies provide the first demonstration that V-PPase may be present on a specific membrane type associated with a plant vacuole, a finding that indicates V-PPase may be required for special vacuole functions. This observation is so far limited specifically to cells in seed embryos because in other experiments we found that antiV-PPase antibodies labeled all vacuoles in root tip cells (Jauh et al., 1999; Jiang et al., 2000). A contribution by V-PPase to specific functions presumably would explain why, in apicomplexian parasites such as trypanosomes and Leishmania, H+-ATPase is present in the lysosome-like vacuole membrane, whereas V-PPase is present in the membrane of acidocalciosomes, which are acidic vacuoles that concentrate calcium (Scott et al., 1998; Rodrigues et al., 1999).
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Materials and methods |
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Antibodies
Monoclonal antibody IgM38 was obtained incidentally during the screen for anti-TIP antibodies; this cross-reacts with an unknown protein in tomato PSV matrix and serves as a matrix marker. Antipeptide antisera were raised in rabbits to sequences from V-PPase proteins: peptide 324, sequence TKAADVGADLVGKIE in putative hydrophilic loop III of Arabidopsis V-PPase (AVP)1 (Sarafian et al., 1992); peptide 1368, sequence EEGVNDQSVVAKCAE of putative hydrophilic loop I of AVP1; and peptide 1401, sequence KGTRDASVKSPVED of putative hydrophilic loop IV of AVP2 (Drozdowicz et al., 2000). These antisera were used at a dilution of 1:100. Transgenic tobacco plants expressing Re-F-B-B were generated and characterized in a manner identical to that described for Re-F-B-
expressing plants (Jiang et al., 2000).
Two different antisera were used to prepare affinity-purified antialeurain antibodies. (a) Antiserum from one of two rabbits immunized with a gel-purified trpE-aleurain fusion protein (Holwerda et al., 1990); this is referred to as rabbit C antiserum. (It should be noted that antibodies from rabbit B antiserum were used to immunoprecipitate proteins in transient expression experiments [Holwerda et al., 1992; Jiang and Rogers, 1998].) (b) Antiserum raised to a His-tagged recombinant aleurain (Rogers et al., 1997); this is referred to as the WSU antiserum. A cDNA clone for the tobacco ortholog of barley aleurain, NTCP-23 (Ueda et al., 2000), was provided by Dr. T. Ueda (National Institute of Agrobiological Resources, Tsukuba, Japan). A His-tagged recombinant protein representing nucleotides 4611117 was expressed in E. coli, purified, and coupled to agarose as described previously for barley aleurain (Rogers et al., 1997). Affinity-purified antibodies from the WSU antiserum were prepared using the recombinant barley aleurain affinity column (Rogers et al., 1997), whereas antibodies were purified from the rabbit C antiserum using the tobacco aleurain column. To further limit the specificity of the WSU antibodies to barley aleurain, they were absorbed with acetone powder (Harlow and Lane, 1988) prepared from tobacco BY-2 suspension culture cells before use in immunofluorescence experiments.
Fractionation of PSVs
Fractions enriched in tonoplast, crystalloid, and globoid were prepared from PSVs isolated from mature dry tomato seeds homogenized in glycerol as described previously for pumpkin PSVs (Jiang et al., 2000) with slight modifications. Sucrose solutions were made per cent wt:wt (Youle and Huang, 1976), and tonoplast was collected from the top of the 30% cushion, crystalloid from the 68% cushion, and purified globoids from the pellet at the bottom of the tube. In an alternative protocol, to minimize possible loss of membrane from the surface of globoids purified PSVs in glycerol were diluted to 80% glycerol with 10 mM Tris-HCl, pH 8, and 1 mM EDTA and mixed with a vortex mixer vigorously at room temperature over a 10-min period. The entire suspension was then layered over a cushion of CCl4 and centrifuged at 4,000 g for 10 min. The supernate was removed and saved, and the interface was then extracted four times by resuspending it in 80% glycerol. The supernates after each centrifugation were saved and pooled. Material collected at the interface after the final wash was defined as globoid because it had a density sufficient to allow it to pellet from 80% glycerol at 4,000 g. Material in the pooled supernates was collected by centrifugation at 10,000 g after dilution to a final glycerol concentration of 25% and was defined as tonoplast plus crystalloid.
EM
For transmission EM, dry tobacco seeds were initially fixed overnight at room temperature in 2% freshly depolymerized paraformaldehyde and 2.5% glutaraldehyde in 70 mM NaCl, 30 mM Hepes, and 2 mM CaCl2, pH 7.4, after slicing off the top and bottom 1020% of the seed to allow better penetration of the fixative. At this point, additional dissection of the seed with a razor blade resulted in easy separation of the embryo from the surrounding seed coat. The embryo was then fixed for an additional 2 h in the same fixative, rinsed three times in distilled water, and post-fixed in either 0.5 or 2.5% potassium permanganate for 2 h at room temperature. After extensive rinsing with water, the tissues were stained en bloc in alkaline bismuth at 40°C for 30 min (Park et al., 1988) before being rinsed in water and dehydrated with an ethanol series. The tissue was infiltrated slowly with an epoxy resin (EmBed 812; Electron Microscopy Sciences). Ultra thin sections were either viewed without further staining or counter stained with uranyl acetate and lead citrate and examined with a JEOL 1200 EX transmission electron microscope.
For immunocytochemistry, mature dry tomato seeds were fixed in 2% freshly depolymerized paraformaldehyde after having the apex and base of their seed coat cut off using a razor blade. After 2 h in the fixative, the seeds were further dissected under a stereoscope to free the cotyledons. After an additional 2 h in fixative, the cotyledons were rinsed en bloc, stained with 0.5% uranyl acetate in ethanol or 25% ethanol for 1 h, and dehydrated with an ethanol series (25, 50, 70, 90, and 3 x 100%) for 10 min each before being infiltrated with LR Gold resin (Electron Microscopy Sciences). Silver sections were picked up on 600 mesh nickel grids, and nonspecific binding sites were blocked by immersion in blocking buffer (BB; 70 mM NaCl, 30 mM Hepes, 0.1% Tween 20, 1% normal goat serum, 0.8% BSA, 0.2% ovalbumin, 0.1% microprotect [Boehringer-Mannheim]) for 115 h. The sections were next immersed in 2040 µl drops of primary monoclonal antibody 351 diluted 1:2,000 in BB for 15 h. After extensive rinsing, the sections were incubated in a goat antimouse immunoglobulin secondary antibody conjugated to 12 nm colloidal gold (Jackson ImmunoResearch Laboratories) diluted 1:40 in BB for 4 h. After rinsing, the sections were counterstained with uranyl acetate and lead citrate. For quantitative studies, randomly chosen globoid cavities were photographed at 40,000 g. In a sandwich immunocytochemical-staining protocol, the colloidal gold particle can be separated from the target antigen by the size of the gold particle plus the 810 nm extended length of both the primary and secondary IgG molecules (Griffiths, 1993). Thus, in our studies any gold particle within a 30-nm radius of the target membrane was counted as specifically bound. The photomicrographs were converted to digital images by high resolution scanning and Adobe Photoshop® used to draw a 60-nm-wide band centered over the outer edge of the globoid cavity or tonoplast membrane. For the crystalloid, the total area of the crystalloid, extending 30 nm outside of its outer edges, was counted. In a small number of cases when two of these regions were closely apposed, a gold particle was located in an area such that it could be counted as belonging to either region. These "double counted" gold particles were <5% of all particles counted. For counting purposes, the location of gold particles was determined by visual inspection of the photographic negatives placed on a light box, since subtle differences in the densities of the globoid contents and colloidal gold could be unequivocally discerned by eye but were sometimes difficult to perceive on the digitized images. The number of gold particles counted was expressed relative to the surface area measured. Statistical analysis of the data was performed using a two-group unpaired Mann-Whitney U-test.
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Footnotes |
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* Abbreviations used in this paper: AVP, Arabidopsis V-PPase; DIP, dark intrinsic protein; LV, lytic vacuole; PAC, precursor-accumulating; PSV, protein storage vacuole; TIP, tonoplast intrinsic protein; V-PPase, vacuolar pyrophosphatase.
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Acknowledgments |
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Submitted: 3 July 2001
Revised: 23 October 2001
Accepted: 26 October 2001
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References |
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