Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854
![]() |
Abstract |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
The transition of laminin from a monomeric
to a polymerized state is thought to be a crucial step in
the development of basement membranes and in the
case of skeletal muscle, mutations in laminin can result
in severe muscular dystrophies with basement membrane defects. We have evaluated laminin polymer and
receptor interactions to determine the requirements for
laminin assembly on a cell surface and investigated
what cellular responses might be mediated by this transition. We found that on muscle cell surfaces, laminins preferentially polymerize while bound to receptors that
included dystroglycan and 7
1 integrin. These receptor interactions are mediated through laminin COOH-terminal domains that are spatially and functionally distinct from NH2-terminal polymer binding sites. This
receptor-facilitated self-assembly drives rearrangement of laminin into a cell-associated polygonal network, a process that also requires actin reorganization
and tyrosine phosphorylation. As a result, dystroglycan
and integrin redistribute into a reciprocal network as
do cortical cytoskeleton components vinculin and dystrophin. Cytoskeletal and receptor reorganization is dependent on laminin polymerization and fails in response to receptor occupancy alone (nonpolymerizing
laminin). Preferential polymerization of laminin on cell
surfaces, and the resulting induction of cortical architecture, is a cooperative process requiring laminin-
receptor ligation, receptor-facilitated self-assembly, actin reorganization, and signaling events.
![]() |
Introduction |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
BASEMENT membranes are a subset of extracellular
matrices made unique by their distinct composition and close association with selected cell surfaces. Laminin and type IV collagen polymers are thought
to be key structural elements of the basement membrane and are usually accompanied by entactin/nidogen, perlecan,
and other glycoproteins (65). The laminins are a family of
large multifunctional molecules thought to be essential for
the development and stability of these specialized matrices. All laminins are heterotrimers composed of individual
,
, and
subunits. Laminins assemble into trimers by
forming a long coiled-coil interaction in the COOH-terminal half of the protein, designated as the long arm, whereas
the NH2-terminal regions remain independent and make up the three short arms. There are currently five laminin
alpha chains, three beta chains, and three gamma chains
that were found in at least 11 different heterotrimer combinations in vivo (37). Depending on the tissue or organ,
different laminin subunits appear to be required at crucial
stages of embryogenesis, as well as for normal function in
mature tissues such as muscle and skin (36, 46, 50).
It is becoming clear that different laminins have both overlapping and unique functions. To understand the mechanisms underlying the requirement for so many laminins, it will be important to determine how these different laminins locally alter the function of basement membranes and exert effects on adjacent cell layers. For instance, it was shown that various laminin isoforms differ in their ability to form polymers. Laminin isoforms 1-4 are capable of forming three-dimensional polymers through interactions among their three NH2-terminal short arms, whereas laminins 5-7, with one or more truncated short arms, are not (7, 48). An independent laminin polymer in conjunction with the collagen IV network provides structure and support for adjacent cells (7, 64). Furthermore, laminins provide these cells with signals and cues through multiple cell surface receptor interactions, influencing processes such as cell survival, proliferation, and migration. However, the relationship and possible interplay between the structural role of laminin (i.e., its architectural state) and its many cell interactive functions has remained unexplored.
In vitro laminin polymerization was shown to be a reversible process in which each of the three short arms interacts to form the basic polymer bond (7). This nucleation/propagation assembly process occurs in solution and
has a critical concentration of 70-140 nm (66). Although
laminins and collagens both form polymers in solution,
laminin polymers are generated through weaker interactions, forming a more plastic polymer better suited to dynamic processes such as tissue remodeling and mechanochemical signaling. Studies using laminin in solution
have been useful for elucidating the domain requirements
and kinetics of laminin polymerization, but alone cannot
describe how the process occurs in a living organism. For
instance, it was observed that basement membranes do not
form in just any empty space between cells, but are found
closely adjacent to particular cell types or even particular
regions of a cell type, such as the basolateral surface of epithelial cells. In fact, basement membrane constituents
may be synthesized by one cell type but end up in the
basement membrane of another, more distal cell type (26).
An earlier study found that as laminin attached to synthetic phospholipid membranes, it polymerized at concentrations well below the solution critical concentration (32).
These results hinted at a mechanism by which laminin- receptor interactions would generate very high local surface concentrations of laminins, thereby driving it above
its critical concentration on the plasma membrane surface.
Supporting a role for receptor interactions in basement
membrane assembly, several mice with mutations in cell
surface receptors were found to have various basement
membrane defects (17, 19, 29, 47, 58). Although these
basement membrane defects were not characterized at the level of laminin polymer formation, and none of these receptors (3 and
1 integrin subunits, and dystroglycan) exclusively binds laminin, together these studies strongly
suggested that cells might use receptors to facilitate and
target laminin polymerization.
Recently, attention has focused on the role of laminin
and its interactions in skeletal muscle. Many severe congenital muscular dystrophies are caused by mutations in
the LAMA2 gene and are marked by basement membrane
defects in both muscle and the nervous system (46). In the
muscle sarcolemmal basement membrane, laminin has
been proposed to provide a linkage between the extracellular matrix and the dystroglycan-glycoprotein complex.
In fact, mutations in virtually any component of the dystroglycan-glycoprotein complex can result in some form
of muscular dystrophy, from Duchenne's (dystrophin) to
muscular dystrophies of the Limb-Girdle type (sarcoglycans; ref. 14). Another binding partner for laminin in skeletal muscle is the 7
1 integrin, particularly at the myotendinous junction. Mutations in the
7 integrin subunit
can also cause skeletal muscle pathologies, although these
appear to be less severe and are classified as myopathies,
not dystrophies (28, 35). Interestingly, several LAMA2
mutations result in the expression of partially functional laminin molecules that maintain their basement membrane localization (1, 42, 49, 55, 59). One such mutation
occurs in the dy2J mouse, where the laminin-2 expressed
by these mice has been found to be defective in its ability
to polymerize, even though it remains localized to the
basement membrane (Colognato, H., and P.D. Yurchenco, manuscript in preparation).
While these disorders have established an important role for basement membranes, the underlying mechanisms behind the requirement for laminins have remained unclear. In the case of the dy2J mouse, laminin remains linked to the cell through receptor interactions and to the basement membrane through its interaction with entactin/ nidogen. Therefore, it seems likely that laminin is required not only to provide an anchor to neighboring tissues, but to somehow alter these tissues, and we should consider that polymerization may play a role in this process. To address these issues it will be important to understand how the many and diverse functions of laminin might cooperate to promote matrix assembly and to provide mechanical and chemical signals to adjacent cells. An emerging paradigm is that the architecture itself transmits information to cells, through mechanisms such as matrix rigidity, spatial arrangement of cell receptors, and tension exerted between the matrix and receptor (8, 18, 20, 33, 51). The structural properties and multiple functions of the laminin molecule would make it well suited to modulate cell interactions through these mechanisms.
In this study, we have evaluated the effects that laminin, as a monomeric or polymeric ligand, has on surface receptors and cortical cytoskeletal components using a muscle cell culture system (60). We present evidence that laminin polymerization occurs preferentially on cell surfaces through specific receptor interactions, providing a targeting mechanism for the assembly of basement membranes. Furthermore, we show that laminin polymerization, above and beyond receptor occupancy, is required to induce reorganization of laminin, its receptors, and cytoskeletal components. This polymer-mediated rearrangement may represent a general mechanism used wherever basement membranes are assembling or remodeling. In fact, a specific disruption in this architecture may be the molecular basis for many congenital muscular dystrophies.
![]() |
Materials and Methods |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Cell Culture
C2C12 myoblasts (American Type Culture Collection) were maintained in DME (Gibco Laboratories) containing 10% FBS in a 37°C incubator with 5% CO2. Subconfluent myoblasts were trypsinized, transferred to 35 × 10 mm tissue culture dishes, and switched to fusion medium upon confluency (DME, 5% horse serum). Myotubes were analyzed 3-5 d after fusion. Cytochalasin D (Sigma Chemical Co.) was used at 0.4 µM and genistein (Calbiochem-Novabiochem Corp.) was used at 25 µM; both inhibitors were added to cultures 2 h before the addition of ligand.
Immunocytochemistry
Fused myotubes were incubated at 37°C with laminins or other proteins suspended in DME containing 0.5% BSA. Unattached protein was removed by washing four times with PBS containing 1 mM CaCl2. Cells were fixed with 3% paraformaldehyde for 10 min at room temperature and, when using antibodies against intracellular epitopes, permeabilized with PBS containing 0.5% Triton X-100 at 0°C. Cells were blocked for 30 min in PBS containing 0.5% BSA and 5% normal goat serum and incubated with primary antibodies diluted in wash buffer (PBS, 0.5% BSA, 0.5% normal goat serum) for 1 h at room temperature. After several washes, cells were incubated with FITC- or rhodamine-conjugated secondary antibodies for 1 h at room temperature. Cells were washed, coverslipped in 1,4 diazabicyclo[2.2.2]octane (Sigma Chemical Co.), and imaged using an Olympus IX-70 inverted fluorescent microscope and a cooled CCD camera (Princeton Instruments Micromax).
Antibodies
Rabbit polyclonal antibodies against mouse laminin-1, human laminin-2,
laminin-1 proteolytic fragments E4, E8, and E3, and recombinant laminin
2-G domain were generated as described previously (13). Anti-E4, anti-E8, anti-E3, and anti-
2(G) were found to be highly specific to these regions of laminins after affinity purification and extensive cross-absorption.
CY8 mouse monoclonal IgG ascites fluid raised against the
7 integrin
subunit was provided by Dr. Randy Kramer (University of California, San
Francisco, CA) and was diluted 1:500 for both blocking experiments and
indirect immunofluorescence. IIH6 mouse monoclonal IgM raised against
rabbit dystroglycan, and used at a 1:2 dilution of hybridoma medium, was
provided by Drs. Hiroki Yamada and Kevin Campbell (Howard Hughes
Medical Institute, University of Iowa, Iowa City, IA). The following antibodies were obtained commercially. A mouse mAb specific for the
COOH-terminal region of
-dystroglycan was used at 1:25 (Novocastra
Laboratories). A hamster monoclonal IgM specific for the
1 integrin
subunit was used at 10-20 µg/ml for blocking experiments, and at 5 µg/ml
for indirect immunofluorescence (PharMingen). Mouse monoclonal IgM
specific for the central domain of dystrophin was used at 1:50 dilution
(Upstate Biotechnology). Mouse monoclonal IgG specific for sarcomeric
-actinin was used at 1:800 dilution (Sigma Chemical Co.). Rabbit polyclonal IgG specific for human fibronectin was used at 1:400 dilution
(Sigma Chemical Co.). Mouse monoclonal IgG specific for vinculin was
used at 1:100 dilution (Sigma Chemical Co.). Rhodamine- and fluorescein-conjugated secondary antibodies specific for mouse IgG, mouse IgM,
hamster IgM, and rabbit IgG were used at the recommended dilutions
(Jackson Immunochemicals and Sigma Chemical Co.).
Proteins
Mouse laminin-1 was extracted from lathyritic EHS tumor and purified as
described (63). A preparation of laminin-2 and -4 (both containing the 2
chain subunit) was prepared from human placenta as described (7), using
a modification of a procedure developed previously (4). Defined fragments of laminin were prepared after digestion of laminin-1 with elastase
or cathepsin G (63). Elastase fragments include: E1', a partial complex of all three NH2-terminal short arms associated with a nidogen fragment; E4,
-chain short arm domains V and VI; E8, distal long arm coiled-coil region and proximal G domain (G repeats 1-3); and E3, distal G domain (G
repeats 4 and 5). The cathepsin G fragment C8-9 is similar to the elastase
fragment E8 but is larger, including most of the coiled coil. Recombinant
laminin
-chain proteins
1(VI-IVb),
2(VI-IVb), and
2(G) were generated and purified as described previously (12, 13, 45). Human fibronectin
was purchased from Sigma Chemical Co.
Protein Analysis
100 mM serine protease inhibitor p-aminoethylbenzenesulfonyl fluoride (AEBSF,1 HCl) was incubated overnight on ice with laminin and used to inactivate laminin self-assembly (see Fig. 4). AEBSF-treated laminin was dialyzed to remove free AEBSF and taken through two rounds of polymerization conditions (37°C, 3 h) to remove any potentially active laminins. [14C]AEBSF (American Radiolabeled Chemicals Inc.) had a specific activity of 55 mCi/mmol. For binding studies, [14C]AEBSF was incubated overnight on ice with laminin and dialysis was performed to remove unbound material. Laminin was digested with elastase (Boehringer Mannheim) using an enzyme/substrate ratio of 1:100 for 24 h at 25°C. Bound [14C]AEBSF was determined using PhosphoImaging (Bio-Rad GS-250; Bio-Rad Laboratories) on fragments separated by SDS-PAGE. Assays to evaluate laminin polymer formation and laminin cell adhesion were performed as previously described (7, 12).
|
|
![]() |
Results |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Laminin Binds to the Myotube Surface and Forms a Network
To understand how laminin interacts with muscle cells, we
evaluated laminins on C2C12 cells, a mouse myogenic cell
line (60). When deprived of mitogens, these cells fuse and
form long, multinucleated myotubes that spontaneously
beat in culture (Fig. 1 f, phase micrograph). Fortunately,
these cells appear to produce only low levels of endogenous laminin, so added laminin was monitored easily. We incubated fused myotubes with laminin-1 (1
1
1) and
found that laminin bound to the dorsal myotube surface
(Fig. 1). Initially (15 min), laminin binding was diffuse
(Fig. 1, a and a'), but later (1 h) appeared aggregated into
a reticular pattern on the surface (Fig. 1, b and b'). By 4 h,
laminin organization on the cell surface appeared predominantly in a repeating, polygonal network with dimensions
generally between 1 and 3 µm (see higher magnification in
c' for greater detail). In addition, these longer incubations
revealed a transition from widespread coverage of laminin
networks to more focal regions of laminin (d shows another myotube at 4 h). This transition was accompanied by
the appearance of compact networks of laminin surrounded by zones with little or no laminin. These regions
maintained the repeating substructure seen in more widespread laminin networks, but the entire architecture was
somewhat more densely packed. Constant replenishment
of the incubation medium with fresh laminin did not alter
this progression from ubiquitous to more focal laminin
coverage, suggesting that a change was occurring in the accessibility, composition, or activation state of laminin receptors. In addition, myotubes incubated in the presence
of laminin for up to 24 h remained healthy and maintained focal laminin networks.
|
Laminin Uses Its COOH-terminal Long Arm to Bind to the Myotube Surface
First, we sought to determine which regions of the large
multisubunit laminin molecule interacted directly with the
myotube cell surface (Fig. 2). A battery of laminin proteolytic fragments and recombinant laminin domains was
evaluated to determine which domains bound directly to
myotube cell surface receptors. Here we considered the
binding properties of both 1- and
2-chain-containing laminins, shown together in a composite model (Fig. 2 A).
This model depicts the boundaries of laminin fragments
and some of the sites known to interact with receptors and
extracellular matrix molecules.
We found that laminin proteins composed of distal
COOH-terminal domains, but not those from NH2-terminal domains, were detected on the cell surface using indirect immunofluorescence (Fig. 2 B). The NH2-terminal
short arm region from both the 1 and
2 laminin subunits
(
1[VI-IVb] and
2[VI-IVb]) have been shown previously to possess active
1
1 and
2
1 integrin binding sites using PC12, HT1080, and primary rat Schwann cells (12). In
contrast, we did not detect any significant level of binding
between C2C12 myotubes and the
1 and
2 laminin short
arms, indicating that fused muscle cells do not directly interact with these NH2-terminal domains. Also, we did not
detect binding of short arm proteolytic fragments E1' and
E4 (data not shown). Proteolytic fragments E8 and E3, derived from the COOH-terminal long arm of mouse laminin-1 (34), bound to the cell surface, remained diffusely
distributed, and had a punctate staining appearance. A recombinant laminin protein consisting of the entire
2 subunit G-domain (45) also bound to the surface and had a
staining pattern similar to that of E3 and E8. Fragment E8
has been shown to mediate laminin binding to the
7
1 integrin (57), whereas fragment E3 has been shown to mediate binding to
-dystroglycan (22). Although both fragments bound to the cell surface, neither formed the large
aggregates and networks seen with intact laminin-1 or
laminin-2. One explanation for this difference could be
that integrin and dystroglycan binding need to occur simultaneously, acting as coreceptors to stimulate surface
aggregation. To test this possibility, we incubated cells with a mixture of E3 and E8. However, the staining pattern remained similar to that seen with individual fragments (not shown), indicating that occupancy of both receptor types was not sufficient to produce larger surface aggregates.
Next, we tested the ability of surface binding proteins or
antibodies to block or alter the organized pattern of intact
laminin on myotube surfaces (Fig. 3). First, cells were incubated for 1 h with COOH-terminal long arm fragments
E3 or E8, or a combination of E3 and E8, or with blocking
antibodies to each of the two principal receptors for laminin in mature muscle fibers, integrin and dystroglycan. Treatment with these reagents was followed by addition of
intact laminin for 1 h. The cells were probed with antibodies specific for the NH2-terminal region of laminin,
thereby only detecting intact laminin bound to the surface
(i.e., not fragments E3 or E8). Surprisingly, function-blocking antibodies against the 1 integrin subunit or the
7 integrin subunit (not shown) had almost no effect on
the ability of laminin to bind to and aggregate on myotube
surfaces. These same antibodies completely blocked fragments E8 and C8-9 (larger than E8, fragment C8-9 also
contains the agrin binding site) from binding to the myotube surface, demonstrating that these antibodies prevent
7
1 integrin binding interactions (not shown). In contrast, treatment with antibody that interferes with laminin-
dystroglycan interactions (mAb IIH6) had a clear effect on
laminin surface aggregation and network formation. Furthermore, fragment E3, which contains the dystroglycan
binding site, significantly reduced laminin binding and aggregation on the myotube surface. Fragment E3 consistently had a greater blocking effect than antidystroglycan. One explanation may be that the E3 fragment contains additional, less prominent receptor binding sites. It is also
possible that a partial or weak blocking effect exerted by
this monoclonal IgM may be responsible for the difference. Nevertheless, a more prominent role in both direct
binding and subsequent aggregation can be attributed to
the E3-mediated interaction(s), compared with those mediated by E8. Finally, incubations combining fragment E3
with fragment E8 now completely blocked laminin from
binding to the myotube surface. The extent of inhibition
seen with single or multiple reagents persisted during
longer laminin incubations: after treatment with laminin
for 4 h, laminin network and cluster formation was significantly blocked by E3, very little by E8, and completely by
a combination of the two (not shown).
|
Laminin Polymerization Is Necessary for Surface Networks to Form
To determine whether laminin-laminin polymer bonds
were required to mediate laminin network formation on a
cell surface, we used several methods to selectively analyze polymer formation. A nonpolymerizing laminin was
generated by treating laminin with AEBSF, a serine protease inhibitor that we fortuitously found has the ability to
bind covalently to laminin (Fig. 4). Binding specificity was
determined by incubating laminin with [14C]AEBSF, followed by the standard elastase proteolysis conditions used
to generate laminin fragments (34). Fig. 4 A shows the relative amount of isotope bound to various laminin elastase
proteolytic fragments. We found that nearly all of the label
was associated with E1', a fragment consisting mostly of
the - and
-NH2-terminal short arms and containing
sites essential for laminin polymer formation (48, 63). In
contrast, the COOH-terminal long arm fragments E3 and
E8 bound little, if any, [14C]AEBSF. Although treatment
with AEBSF abolished laminin's ability to form a polymer
in solution (Fig. 4 B), its ability to interact with surface receptors (including
1
1,
2
1,
3
1,
6
1, and
7
1 integrins) in the cell types we have tested so far (HT1080, C2C12, 1072Sk fibroblasts, and primary Schwann cells) remained unchanged. Adhesion of C2C12 myoblasts to both
untreated and AEBSF-treated laminin-1 is shown in Fig. 4
C. In addition, we found that the dystroglycan-binding
fragment E3 retained its ability to interact with the muscle
cell surface after treatment with AEBSF (not shown) and
that the affinity of laminin for dystroglycan remained unchanged after AEBSF treatment (Combs, A., and J. Ervasti, personal communication). Essentially, this reagent
produced a nonpolymerizing but otherwise functional
laminin molecule, and allowed us to test directly the role
of laminin polymer formation independent of its cell adhesive functions.
We found that nonpolymerizing AEBSF-laminin was unable to form aggregates and networks on the cell surface (Fig. 5, AEBSF-Lm). Conditions used to disrupt laminin polymer bonds selectively in solution (48) also were found to disrupt laminin cell surface organization, but had no effect on the binding of surface receptors (Fig. 5). Specifically, molar excess of either laminin short arm proteolytic fragments E1'or E4 prevented formation of laminin surface aggregates that appear after 1 h. Inhibition of polymerization also prevented development of laminin networks and clusters seen after longer incubations. After 4 h, polymerization-competent laminin appeared in both widespread networks and more compact clusters. However, AEBSF-treated laminin, or laminin blocked with fragment E1' did not form these structures.
|
Laminin Polymerization Is Required for Induction of a Cortical Network of Receptors and Cytoskeletal Components
The organization of receptors and cortical cytoskeleton in
the presence or absence of laminin was evaluated. Treatment of myotubes for 4 h with polymer-competent laminin
resulted in the rearrangement of 7
1 integrin, dystroglycan, dystrophin, and cortical vinculin, but not
-actinin
(Fig. 6). Laminin-treated myotubes were double-labeled
to visualize the laminin network, sarcolemmal proteins (
7
and
1 integrin subunits, dystroglycan, vinculin, dystrophin, and
-actinin) and their overlapping distributions (Fig. 6, merge shown on left). Integrin (
7 and
1 subunits), dystroglycan, vinculin, and dystrophin formed repeating polygonal structures similar in appearance and location to those formed by laminin. Beneath the laminin
network, both receptor types also codistributed (Fig. 6,
dystroglycan and integrin double labeling). Myotubes incubated with BSA and those incubated with nonpolymerizing laminin are shown in the bottom two rows of Fig. 6.
The organization of
1 integrin, dystroglycan, vinculin,
and dystrophin was very different in these myotubes, appearing diffusely distributed with regions of small clusters.
Therefore, laminin that could engage receptors, but could
not form surface polymers, was insufficient to induce receptor and cytoskeleton rearrangements.
|
Actin Reorganization and Tyrosine Phosphorylation Are Required for the Formation of Laminin Cell Surface Networks
The architecture of the laminin polymer, with its ~35-nm strut meshwork, can be appreciated only at the level of electron microscopy. While triggered by polymerization, the organized semi-regular appearance of the laminin surface network suggested that creation of this architecture might be an active process perhaps requiring remodeling of the cortical actin network, or even signaling events. We investigated the role of actin by comparing the organization of laminin on myotubes incubated in the presence or absence of cytochalasin, an agent that prevents assembly and disassembly of filamentous actin (Fig. 7). In contrast to control myotubes, cytochalasin-treated myotubes did not show prominent rearrangement of surface laminin into complex networks, even after 4 h. On cytochalasin-treated myotubes, laminin coverage remained widespread and had a near continuous, almost mattelike quality. Inspection at higher magnification (Fig. 7, insets) revealed a delicate substructure to regions of this laminin covering, one that was very different from the larger, more sharply delineated polygonal units seen in control cultures. In addition, the clearance of laminin from large regions of the myotube surface was inhibited.
|
Next, we evaluated whether energy-dependent signaling cascades might be required to promote laminin network formation on the cell surface. We found that genistein, a general inhibitor of protein tyrosine kinases, had a large effect on laminin's ability to form a surface network (Fig. 7). On genistein-treated myotubes, laminin coverage appeared ubiquitous and without much complexity, similar to that seen on cytochalasin-treated cells. In contrast, several other inhibitors of signaling molecules had virtually no effect on laminin network formation (not shown). These were wortmannin, an irreversible inhibitor of phophatidylinositol 3-kinase, and ML-7 hydrochloride, a selective inhibitor of myosin light chain kinase that is often used to interfere with actin-myosin contractility. The observed requirement for actin remodeling suggested a role for the Rho family of small GTPases (27). To address this possibility, we used C3 transferase, a selective inhibitor of Rho, but this treatment was found to disrupt myotube adhesion and structure grossly. Therefore, it could not be used to evaluate the role of Rho-mediated signaling in laminin surface network formation.
Laminin Expressed by dy2J/dy2J Mice Is Defective in Forming Surface Networks
Mice homozygous for the dy2J LAMA2 allele were found
to have a point mutation within the 5' coding region that
causes an abnormal splicing event (55, 59). This genetic
defect causes a severe and progressive muscular dystrophy
thought to be analogous to the many human congenital
muscular dystrophies caused by LAMA2 mutations. Because of irregular splicing, dy2J/dy2J mice express a mutated laminin 2 chain lacking 57 amino acids within domain VI, the most NH2-terminal short arm domain (see laminin map, Fig. 2 A). Nonetheless, this aberrant laminin
subunit forms a heterotrimer with the
and
chains, is
secreted, and is localized to the sarcolemmal basement membrane.
Subsequently, it has been found that the 2-chain-containing laminins (a combination of laminins-2 and -4) from
the skeletal muscle of dy2J/dy2J mice is defective in its ability to form a polymer in solution (Colognato, H., and P.D.
Yurchenco, manuscript in preparation). This polymerization defect most likely accounts for the dystrophy seen in
these mice since receptor recognition sites in the laminin
2 domain VI do not seem to be used in mature skeletal
muscle. In vivo, this mutant laminin appears to be held
within the basement membrane solely through linkage to
the collagen IV network, since we find that digestion of
muscle tissue with collagenase liberates dy2J-laminin into
solution. Normally, muscle laminin requires an additional treatment with EDTA to disrupt the calcium-dependent
laminin polymer.
In Addition to Providing Insight into the Mechanism of LAMA2 Muscular Dystrophies, dy2J-Laminin Provides an Opportunity to Study a Naturally Occurring, Polymer-defective Laminin
We purified 2-laminin extracted from wild-type and dy2J/
dy2J muscle and evaluated the ability of these laminins to
attach to muscle cell receptors and organize into a surface
network (Fig. 8 A). Myotubes incubated for 4 h with wild-type mouse
2-laminin showed extensive formation of
laminin surface networks. The inset at higher magnification (3×) clearly shows the characteristic laminin network
architecture seen with laminin-1. On average, the spacing
of repeating polygonal units was often more compact when compared with that of laminin-1, but the unit size
range was similar for both. In contrast, dy2J-
2-laminin
bound to the myotube surface but did not form these organized structures, remaining in a diffusely distributed punctate pattern. This pattern was similar to that seen with
nonpolymerizing laminin-1 (Fig. 5).
|
Polymer-defective dy2J-2-Laminin Fails to Induce
Reorganization of Receptors and Cortical
Cytoskeletal Proteins
Myotubes were incubated with either wild-type or dy2J-
2-laminin and visualized by indirect immunofluorescence. Double staining revealed the location and relationship of laminin and the following sarcolemmal proteins:
1
integrin subunit, dystroglycan, and vinculin (Fig. 8 B). Induction of corresponding integrin, dystroglycan, and vinculin networks was observed in the presence of wild-type
2-laminin, but dy2J-
2-laminin failed to induce this reorganization. As in the presence of nonpolymerizing laminin-1, laminin receptors remained in a diffusely distributed, punctate pattern, as did vinculin. In addition, the
intensity of vinculin staining at the cortical region appeared to be greater in myotubes incubated with polymerizing laminin-1 or -2, suggesting that vinculin was being recruited into the cortex and reorganized.
![]() |
Discussion |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
The extracellular matrix molecule laminin has been described previously as having two major, though independent, functions: (1) a ligand for cell surface receptors,
mediating such processes as neurite outgrowth and prevention of apoptosis, and (2) a structural molecule that
forms a polymer and is required for basement membrane
architecture, providing mechanical support to adjacent cells. In this study, we have presented evidence that these
two types of functions are in fact integrated, acting synergistically to reorganize the cell surface and adjacent cortical cytoskeleton (Fig. 9). It seems likely that this laminin-induced process represents a specific mechanism for the
transmission of complex cellular signals. For these cells,
this mechanism appears to be laminin-specific, not induced by the addition of fibronectin, collagen IV, or even by the addition of divalent antibodies to artificially cluster nonpolymerizing laminin (not shown). We have shown
that laminin attaches to the cell surface while bound to
dystroglycan, integrin 7
1, and possibly other receptors
for the laminin long arm. The concentration of receptor-engaged laminin is thereby selectively increased, now
exceeding the critical concentration for laminin self-assembly and driving polymerization preferentially on cell surfaces (receptor-facilitated self-assembly). Furthermore,
through cooperative polymer and receptor interactions,
laminin organizes into complex polygonal arrays on the
cell surface. This appears to be an active process that requires remodeling of actin filaments and tyrosine kinase
signaling. Laminin polymerization induces reorganization
of laminin receptors, both integrin and dystroglycan, and
elements of the cortical cytoskeleton, vinculin and dystrophin. Moreover, we find that a mutated laminin causing
muscular dystrophy and dysmyelination in the dy2J/dy2J
mouse is defective in its ability to form a surface network
and to induce these specific changes in the sarcolemmal
architecture.
|
Laminin at the Muscle Sarcolemma: Receptor Ligation Is Not Sufficient for Induction
In the past few years, numerous mutations in the LAMA2
gene have been identified as the cause of congenital muscular dystrophy (CMD, ref. 53). Furthermore, the hallmark
of this emerging class of disorders is the accompaniment of
peripheral and central nervous system abnormalities, indicating a requirement for this laminin beyond the skeletal
musculature. Although the isolation of these laminin
mutations offers a significant breakthrough in the understanding of these congenital disease processes, insight into
the molecular mechanism has been elusive. Many identified mutations result in absence of the laminin 2 protein
altogether, but may be accompanied to varying degrees by
upregulation of additional laminin chains such as
4 and
5, whose degree of overlapping function remains unclear
(43). Mutations resulting in expressed protein with specific
functional deletions, a smaller group, may lead to a more
selective analysis of the contributions of specific laminin functions. Since laminin isoforms have many overlapping
functions, structure/function correlation may permit identification of laminin candidates that could be upregulated
or expressed to correct
2-deficient CMDs, similar to the
rescue of dystrophin-negative dystrophic mdx mice using
the dystrophin homologue, utrophin (44, 56).
The dystrophic dy2J mouse has an abnormally spliced
laminin 2 transcript resulting from a point mutation in a
splice donor site (55, 59). The resulting
2 protein is expressed, incorporated into a laminin heterotrimer, and
localized to the sarcolemmal basement membrane. This
2-chain short arm defect in domain VI is analogous to
several human CMDs that express partially functional
2
proteins with defects in the NH2-terminal short arm (1, 11,
42). In this study, we find that fused muscle cells bind to
laminin through receptor-binding sites in the COOH-terminal long arm, ~110 nm away from the short arm region
that is defective in polymer formation (Colognato, H., and
P.D. Yurchenco, manuscript in preparation). This laminin
remains within the basement membrane through interactions with cell surface receptors and through linkage to the
type IV collagen network, the latter mediated by entactin/ nidogen. In this context, it seemed unlikely that the prime
role of laminin polymerization is simply to dock laminin
into the basement membrane and provide a mechanical
tether. Therefore, we sought to understand how laminin
polymerization might alter the muscle sarcolemma in such
a way that could promote muscle function and, ultimately,
survival. The novel laminin function that we report here
seems a likely candidate: the induction of dramatic changes in organization of matrix, receptors, and cortical cytoskeletal components in response to laminin polymerization. This induction may underlie a key requirement for
laminin polymerization in maintenance of proper cellular
architecture and function.
A Role for Ligand Architecture above and beyond Receptor Occupancy
Signaling pathways mediated by some growth factors not only have been found to be dependent on ligand occupancy of receptors, but also on dimerization of these receptors (3, 9, 62). In a related concept, receptor aggregation, mediated by fibronectin fibrils, is thought to recruit a large collection of cytoskeletal components and signaling molecules to these sites (38, 61). For this process, it has been shown that ligand occupancy alone (provided by RGD peptide) activates only a small subset of the responses mediated by a fibrillar fibronectin network. Similar or related mechanisms might play a role in laminin signaling, although laminin differs significantly from fibronectin in its distribution, receptor partners, and mode of supramolecular assembly. For instance, fibronectin forms a fibrillar rather than a meshlike polymer and its assembly process does not occur in the absence of receptor interactions. This difference turned out to be helpful to our study, as we were able to evaluate laminin's polymerization process independent of receptor occupancy and assess the extent to which laminin-induced changes were polymer-induced.
Traditionally, basement membranes have been thought of as supportive substrates for adjacent cells that enable cell adhesion or migration. The data presented here suggest a more dynamic, instructive role for the supramolecular organization of laminin in the basement membrane. Evidence has been building in recent years for the idea that cellular architecture has the ability to transmit information. At the forefront of this paradigm has been the concept of tensegrity, a model for mechanotransduction in which cells respond to mechanical stresses and changes in the physical environment through a matrix receptor-cytoskeleton nuclear continuum (30). In support of this model, spatial arrangement of matrix molecules has been shown to mediate processes such as proliferation, apoptosis, and arrangement of cytoskeletal architecture (6, 31, 33, 40). In this study we have shown that a change in laminin architecture is able to instruct muscle cells, leading to changes in receptor and cytoskeletal architecture. Interestingly, preliminary data suggest that laminin polymerization may activate tyrosine phosphorylation above and beyond that seen with nonpolymerizing laminin (Hanus, C., and P.D. Yurchenco, unpublished observations).
Studies in which certain laminin domains have been
masked or proteolytically altered also support the notion
that laminin architecture has the ability to influence cellular responses (5, 25). In these studies, the modified laminin
domains were completely distinct from those regions interacting with the cell receptor(s) in question. In a study
done by Giannelli and colleagues, it was found that proteolytic cleavage in the short arm of the 2 subunit transformed cell interactions mediated by the G-domain of
laminin-5, converting a static adhesion activity to an invasive, migratory activity used by cancer cells. The mechanism for this functional switching remains unclear, but
information transmitted through the architectural arrangement of these ligands may very well contribute.
Cooperativity between Laminin Polymerization and Laminin-Receptor Interactions
The relationship between laminin-receptor interactions
and basement membrane formation has been examined in
recent years. Targeted gene disruptions of the 1 integrin
subunit and dystroglycan, both lethal to embryonic development, have indicated a requirement for receptors in the
proper assembly and subsequent function of embryonic
basement membranes (19, 52, 58). Furthermore, mutations in the
3,
6, and
4 integrin subunits cause defects in the epidermal basement membrane of the skin, often
leading to epidermolysis bullosa (17, 24, 41). Teratomas
formed by
1-null embryonic stem cells injected into normal mice formed aberrant basement membranes that appeared to develop and slough off of adjacent cells, often in
multiple and abnormally thickened layers (2, 47). Dystroglycan-null embryos die after implantation after failing to
develop a functional extraembryonic Reichert's membrane, allowing maternal blood into the yolk sac cavity.
Examination of this specialized basement membrane revealed only occasional patchy regions of laminin and collagen IV staining instead of the continuous staining observed normally. Furthermore, recent work has shown that
dystroglycan
/
embryonic stem cells grown in culture to
form embryoid bodies also have defects in basement membrane assembly (29).
How does the removal of dystroglycan, or other laminin receptors, lead to such profoundly defective basement membranes? Other molecular bonds, such as laminin-integrin or laminin-nidogen-collagen IV, could presumably be sufficient to retain laminin within the basement membrane of dystroglycan-null cells. This suggests that the laminin- dystroglycan interaction may provide an inductive role to cells in addition to providing a simple mechanical linkage. The ability of dystroglycan binding fragment E3 to disrupt markedly not only laminin surface binding but also its surface assembly is consistent with this hypothesis. The large effect seen by inhibiting dystroglycan, versus integrin, interaction sites may be due to a larger reduction in laminin accumulation on the surface, or a greater mobility of dystroglycan through the lipid bilayer compared with integrin, but it could also indicate an inductive role specific to the laminin-dystroglycan interaction.
The laminin-dystroglycan relationship also has been examined in the context of neuromuscular junction formation. It has been found that AChR receptor clustering is mediated in part by neural agrin through a mechanism dependent on the tyrosine kinase receptor MuSK (15, 21). Agrin, which has laminin-G domainlike repeats, has been shown to bind to both laminin and dystroglycan (16, 23). It has been suggested that laminin and dystroglycan may each serve as reservoirs for agrin at the neuromuscular endplate, perhaps facilitating agrin's ability to induce AChR clustering. Moreover, recent work has illustrated a MuSK-independent AChR clustering activity that is activated by the addition of exogenous laminin (54). In fact, the laminin-dystroglycan interaction specifically was required for this laminin-induced AChR clustering to occur (39). However, an earlier study by Cohen and colleagues found that by adding laminin to Xenopus muscle cultures, small dystroglycan clusters are converted to larger more focal clusters but that these laminin-induced clusters were not accompanied by accumulation of AChR (10). Using the C2C12 muscle culture system, we do observe an increase in AChR clusters upon addition of laminin, but only a subset of these clusters colocalizes with laminin (micrographs not shown). It should be interesting to learn whether the receptor and cytoskeletal rearrangements induced by laminin polymerization play a role in signaling this MuSK-independent pathway for AChR clustering.
In summary, we present evidence that laminin polymerization induces a cortical cellular architecture comprised of matrix, receptor, and cytoskeletal elements. This process requires a unique synergism between laminin architecture-forming and receptor-ligating functions, mediated by distal NH2-terminal and COOH-terminal epitopes, respectively. Furthermore, we demonstrate that a mutant laminin responsible for CMD and nervous system defects is defective in its ability to form surface networks and subsequently induce receptor-cytoskeleton rearrangement. This organizational signaling induced by laminin introduces a novel mechanism by which the supramolecular configuration of basement membranes instructs adjacent cells and tissues.
![]() |
Footnotes |
---|
Address correspondence to Peter D. Yurchenco, Robert Wood Johnson Medical School, Department of Pathology, 675 Hoes Lane, Piscataway, NJ 08854. Tel.: (732) 235-4674. Fax: (732) 235-4825. E-mail: yurchenc{at}rwja.umdnj.edu
Received for publication 17 September 1998 and in revised form 1 March 1999.
This project was supported by National Institutes of Health grants R01-DK36425 (to P.D. Yurchenco) and R01-AR38454 (to D.A. Winkelmann).
We thank Ms. Ariana Combs and Dr. James Ervasti for evaluating the dystroglycan-binding activity of AEBSF-treated laminin. We thank Dr. Hiroki Yamada and Dr. Kevin Campbell (IIH6) and Dr. Randy Kramer (CY8) for their gifts of antibodies.
![]() |
Abbreviations used in this paper |
---|
AEBSF, p-aminoethylbenzenesulfonyl fluoride; CMD, congenital muscular dystrophy.
![]() |
References |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
1. |
Allamand, V.,
Y. Sunada,
M.A. Salih,
V. Straub,
C.O. Ozo,
M.H. Al-Turaiki,
M. Akbar,
T. Kolo,
H. Colognato,
X. Zhang,
L.M. Sorokin,
P.D. Yurchenco, et al
.
1997.
Mild congenital muscular dystrophy in two patients with an internally deleted laminin alpha2-chain.
Hum. Mol. Genet.
6:
747-752
|
2. |
Bloch, W.,
E. Forsberg,
S. Lentini,
C. Brakebusch,
K. Martin,
H.W. Krell,
U.H. Weidle,
K. Addicks, and
R. Faessler.
1997.
![]() |
3. | Boni-Schnetzler, M., and P.F. Pilch. 1987. Mechanism of epidermal growth factor receptor autophosphorylation and high-affinity binding. Proc. Natl. Acad. Sci. USA. 84: 7832-7836 [Abstract]. |
4. |
Brown, J.C.,
H. Wiedemann, and
R. Timpl.
1994.
Protein binding and cell
adhesion properties of two laminin isoforms (AmB1eB2e, AmB1sB2e)
from human placenta.
J. Cell Sci.
107:
329-338
|
5. | Calof, A.L., M.R. Campanero, J.J. O'Rear, P.D. Yurchenco, and A.D. Lander. 1994. Domain-specific activation of neuronal migration and neurite outgrowth-promoting activities of laminin. Neuron. 13: 117-130 |
6. |
Chen, C.S.,
M. Mrksich,
S. Huang,
G.M. Whitesides, and
D.E. Ingber.
1997.
Geometric control of cell life and death.
Science.
276:
1425-1428
|
7. |
Cheng, Y.S.,
M.F. Champliaud,
R.E. Burgeson,
M.P. Marinkovich, and
P.D. Yurchenco.
1997.
Self-assembly of laminin isoforms.
J. Biol. Chem.
272:
31525-31532
|
8. | Choquet, D., D.P. Felsenfeld, and M.P. Sheetz. 1997. Extracellular matrix rigidity causes strengthening of integrin-cytoskeleton linkages. Cell. 88: 39-48 |
9. |
Cochet, C.,
O. Kashles,
E.M. Chambaz,
I. Borrello,
C.R. King, and
J. Schlessinger.
1988.
Demonstration of epidermal growth factor-induced
receptor dimerization in living cells using a chemical covalent cross-linking agent.
J. Biol. Chem.
263:
3290-3295
|
10. |
Cohen, M.W.,
C. Jacobson,
P.D. Yurchenco,
G.E. Morris, and
S. Carbonetto.
1997.
Laminin-induced clustering of dystroglycan on embryonic
muscle cells: comparison with agrin-induced clustering.
J. Cell Biol.
136:
1047-1058
|
11. | Cohn, R.D., R. Herrmann, L. Sorokin, U.M. Wewer, and T. Voit. 1998. Laminin alpha2 chain-deficient congenital muscular dystrophy: variable epitope expression in severe and mild cases. Neurology 51: 94-100 [Abstract]. |
12. |
Colognato-Pyke, H.,
J.J. O'Rear,
Y. Yamada,
S. Carbonetto,
Y.S. Cheng, and
P.D. Yurchenco.
1995.
Mapping of network-forming, heparin-binding, and alpha 1 beta 1 integrin-recognition sites within the alpha-chain
short arm of laminin-1.
J. Biol. Chem
270:
9398-9406
|
13. |
Colognato, H.,
M. MacCarrick,
J.J. O'Rear, and
P.D. Yurchenco.
1997.
The laminin alpha2-chain short arm mediates cell adhesion through both
the alpha1beta1 and alpha2beta1 integrins.
J. Biol. Chem
272:
29330-29336
|
14. | Culligan, K.G., A.J. Mackey, D.M. Finn, P.B. Maguire, and K. Ohlendieck. 1998. Role of dystrophin isoforms and associated proteins in muscular dystrophy (review). Int. J. Mol. Med. 2: 639-648 . |
15. | DeChiara, T.M., D.C. Bowen, D.M. Valenzuela, M.V. Simmons, W.T. Poueymirou, S. Thomas, E. Kinetz, D.L. Compton, E. Rojas, J.S. Park, et al . 1996. The receptor tyrosine kinase MuSK is required for neuromuscular junction formation in vivo. Cell. 85: 501-512 |
16. |
Denzer, A.J.,
R. Brandenberger,
M. Gesemann,
M. Chiquet, and
M.A. Ruegg.
1997.
Agrin binds to the nerve-muscle basal lamina via laminin.
J. Cell Biol.
137:
671-683
|
17. |
DiPersio, C.M.,
K.M. Hodivala-Dilke,
R. Jaenisch,
J.A. Kreidberg, and
R.O. Hynes.
1997.
![]() ![]() |
18. | Ezzell, R.M., W.H. Goldmann, N. Wang, N. Parasharama, and D.E. Ingber. 1997. Vinculin promotes cell spreading by mechanically coupling integrins to the cytoskeleton. Exp. Cell Res. 231: 14-26 |
19. | Fassler, R., and M. Meyer. 1995. Consequences of lack of beta 1 integrin gene expression in mice. Genes Dev. 9: 1896-1908 [Abstract]. |
20. | Felsenfeld, D.P., D. Choquet, and M.P. Sheetz. 1996. Ligand binding regulates the directed movement of beta1 integrins on fibroblasts. Nature. 383: 438-440 |
21. | Gautam, M., P.G. Noakes, L. Moscoso, F. Rupp, R.H. Scheller, J.P. Merlie, and J.R. Sanes. 1996. Defective neuromuscular synaptogenesis in agrin-deficient mutant mice. Cell. 85: 525-535 |
22. |
Gee, S.H.,
R.W. Blacher,
P.J. Douville,
P.R. Provost,
P.D. Yurchenco, and
S. Carbonetto.
1993.
Laminin-binding protein 120 from brain is closely
related to the dystrophin-associated glycoprotein, dystroglycan, and
binds with high affinity to the major heparin binding domain of laminin.
J. Biol. Chem.
268:
14972-14980
|
23. | Gee, S.H., F. Montanaro, M.H. Lindenbaum, and S. Carbonetto. 1994. Dystroglycan-alpha, a dystrophin-associated glycoprotein, is a functional agrin receptor. Cell. 77: 675-686 |
24. | Georges-Labouesse, E., N. Messaddeq, G. Yehia, L. Cadalbert, A. Dierich, and M. Le Meur. 1996. Absence of integrin alpha 6 leads to epidermolysis bullosa and neonatal death in mice. Nat. Genet. 13: 370-373 |
25. |
Giannelli, G.,
J. Falk-Marzillier,
O. Schiraldi,
W.G. Stetler-Stevenson, and
V. Quaranta.
1997.
Induction of cell migration by matrix metalloprotease-2 cleavage of laminin-5.
Science.
277:
225-228
|
26. |
Graham, P.L.,
J.J. Johnson,
S. Wang,
M.H. Sibley,
M.C. Gupta, and
J.M. Kramer.
1997.
Type IV collagen is detectable in most, but not all, basement membranes of Caenorhabditis elegans and assembles on tissues that
do not express it.
J. Cell Biol.
137:
1171-1183
|
27. |
Hall, A..
1998.
Rho GTPases and the actin cytoskeleton.
Science.
279:
509-514
|
28. | Hayashi, Y.K., F.L. Chou, E. Engvall, M. Ogawa, C. Matsuda, S. Hirabayashi, K. Yokochi, B.L. Ziober, R.H. Kramer, S.J. Kaufman, et al . 1998. Mutations in the integrin alpha7 gene cause congenital myopathy. Nat. Genet. 19: 94-97 |
29. | Henry, M.D., and K.P. Campbell. 1998. A role for dystroglycan in basement membrane assembly. Cell. 95: 859-870 |
30. | Ingber, D.E.. 1997. Tensegrity: the architectural basis of cellular mechanotransduction. Annu. Rev. Physiol. 59: 575-599 |
31. | Ingber, D.E., D. Prusty, Z. Sun, H. Betensky, and N. Wang. 1995. Cell shape, cytoskeletal mechanics, and cell cycle control in angiogenesis. J. Biomech. 28: 1471-1484 |
32. |
Kalb, E., and
J. Engel.
1991.
Binding and calcium-induced aggregation of
laminin onto lipid bilayers.
J. Biol. Chem.
266:
19047-19052
|
33. |
Maniotis, A.J.,
C.S. Chen, and
D.E. Ingber.
1997.
Demonstration of mechanical connections between integrins, cytoskeletal filaments, and nucleoplasm that stabilize nuclear structure.
Proc. Natl. Acad. Sci. USA.
94:
849-854
|
34. | Mann, K., R. Deutzmann, and R. Timpl. 1988. Characterization of proteolytic fragments of the laminin-nidogen complex and their activity in ligand-binding assays. Eur. J. Biochem. 178: 71-80 [Abstract]. |
35. | Mayer, U., G. Saher, R. Fassler, A. Bornemann, F. Echtermeyer, H. von der Mark, N. Miosge, E. Poschl, and K. von der Mark. 1997. Absence of integrin alpha 7 causes a novel form of muscular dystrophy. Nat. Genet. 17: 318-323 |
36. |
Miner, J.H.,
B.L. Patton,
S.I. Lentz,
D.J. Gilbert,
W.D. Snider,
N.A. Jenkins,
N.G. Copeland, and
J.R. Sanes.
1997.
The laminin alpha chains: expression, developmental transitions, and chromosomal locations of ![]() ![]() |
37. |
Miner, J.H.,
J. Cunningham, and
J.R. Sanes.
1998.
Roles for laminin in embryogenesis: exencephaly, syndactyly, and placentopathy in mice lacking
the laminin alpha5 chain.
J. Cell Biol.
143:
1713-1723
|
38. | Miyamoto, S., S.K. Akiyama, and K.M. Yamada. 1995. Synergistic roles for receptor occupancy and aggregation in integrin transmembrane function. Science. 267: 883-885 |
39. |
Montanaro, F.,
S.H. Gee,
C. Jacobson,
M.H. Lindenbaum,
S.C. Froehner, and
S. Carbonetto.
1998.
Laminin and alpha-dystroglycan mediate acetylcholine receptor aggregation via a MuSK-independent pathway.
J.
Neurosci.
18:
1250-1260
|
40. | Mooney, D., L. Hansen, J. Vacanti, R. Langer, S. Farmer, and D. Ingber. 1992. Switching from differentiation to growth in hepatocytes: control by extracellular matrix. J. Cell. Physiol. 151: 497-505 |
41. |
Niessen, C.M.,
M.H. van der Raaij-Helmer,
E.H. Hulsman,
R. van der
Neut,
M.F. Jonkman, and
A. Sonnenberg.
1996.
Deficiency of the integrin beta 4 subunit in junctional epidermolysis bullosa with pyloric atresia: consequences for hemidesmosome formation and adhesion properties [published erratum appears in J. Cell Sci. 1996. 109(8):preceding
table of contents].
J. Cell Sci.
109:
1695-1706
|
42. | Nissinen, M., A. Helbling-Leclerc, X. Zhang, T. Evangelista, H. Topaloglu, C. Cruaud, J. Weissenbach, M. Fardeau, F.M. Tome, K. Schwartz, K. Tryggvason, and P. Guicheney. 1996. Substitution of a conserved cysteine-996 in a cysteine-rich motif of the laminin alpha2-chain in congenital muscular dystrophy with partial deficiency of the protein. Am. J. Hum. Genet. 58: 1177-1184 |
43. |
Patton, B.L.,
J.H. Miner,
A.Y. Chiu, and
J.R. Sanes.
1997.
Distribution and
function of laminins in the neuromuscular system of developing, adult,
and mutant mice.
J. Cell Biol.
139:
1507-1521
|
44. | Rafael, J.A., J.M. Tinsley, A.C. Potter, A.E. Deconinck, and K.E. Davies. 1998. Skeletal muscle-specific expression of a utrophin transgene rescues utrophin-dystrophin deficient mice. Nat. Genet. 19: 79-82 |
45. | Rambukkana, A., J.L. Salzer, P.D. Yurchenco, and E.I. Tuomanen. 1997. Neural targeting of Mycobacterium leprae mediated by the G domain of the laminin-alpha2 chain. Cell. 88: 811-821 |
46. | Ryan, M.C., A.M. Christiano, E. Engvall, U.M. Wewer, J.H. Miner, J.R. Sanes, and R.E. Burgeson. 1996. The functions of laminins: lessons from in vivo studies. Matrix Biol. 15: 369-381 |
47. | Sasaki, T., E. Forsberg, W. Bloch, K. Addicks, R. Fassler, and R. Timpl. 1998. Deficiency of beta 1 integrins in teratoma interferes with basement membrane assembly and laminin-1 expression. Exp. Cell Res. 238: 70-81 |
48. | Schittny, J.C., and P.D. Yurchenco. 1990. Terminal short arm domains of basement membrane laminin are critical for its self-assembly. J. Cell Biol. 110: 825-832 [Abstract]. |
49. | Sewry, C.A., I. Naom, M. D'Alessandro, L. Sorokin, S. Bruno, L.A. Wilson, V. Dubowitz, and F. Muntoni. 1997. Variable clinical phenotype in merosin-deficient congenital muscular dystrophy associated with differential immunolabeling of two fragments of the laminin alpha 2 chain. Neuromuscul. Disord 7: 169-175 |
50. |
Smyth, N.,
H.S. Vatansever,
P. Murray,
M. Meyer,
C. Frie,
M. Paulsson, and
D. Edgar.
1999.
Absence of basement membranes after targeting the
LAMC1 gene results in embryonic lethality due to failure of endoderm
differentiation.
J. Cell Biol.
144:
151-160
|
51. | Stamenovic, D., J.J. Fredberg, N. Wang, J.P. Butler, and D.E. Ingber. 1996. A microstructural approach to cytoskeletal mechanics based on tensegrity. J. Theor. Biol. 181: 125-136 |
52. | Stephens, L.E., A.E. Sutherland, I.V. Klimanskaya, A. Andrieux, J. Meneses, R.A. Pedersen, and C.H. Damsky. 1995. Deletion of beta 1 integrins in mice results in inner cell mass failure and peri-implantation lethality. Genes Dev. 9: 1883-1895 [Abstract]. |
53. | Straub, V., and K.P. Campbell. 1997. Muscular dystrophies and the dystrophin-glycoprotein complex. Curr. Opin. Neurol. 10: 168-175 |
54. |
Sugiyama, J.E.,
D.J. Glass,
G.D. Yancopoulos, and
Z.W. Hall.
1997.
Laminin-induced acetylcholine receptor clustering: an alternative pathway.
J.
Cell Biol.
139:
181-191
|
55. | Sunada, Y., S.M. Bernier, A. Utani, Y. Yamada, and K.P. Campbell. 1995. Identification of a novel mutant transcript of laminin alpha 2 chain gene responsible for muscular dystrophy and dysmyelination in dy2J mice. Hum. Mol. Genet. 4: 1055-1061 [Abstract]. |
56. | Tinsley, J.M., A.C. Potter, S.R. Phelps, R. Fisher, J.I. Trickett, and K.E. Davies. 1996. Amelioration of the dystrophic phenotype of mdx mice using a truncated utrophin transgene. Nature. 384: 349-353 |
57. |
von der Mark, H.,
J. Durr,
A. Sonnenberg,
K. von der Mark,
R. Deutzmann, and
S.L. Goodman.
1991.
Skeletal myoblasts utilize a novel beta
1-series integrin and not alpha 6 beta 1 for binding to the E8 and T8 fragments of laminin.
J. Biol. Chem.
266:
23593-23601
|
58. |
Williamson, R.A.,
M.D. Henry,
K.J. Daniels,
R.F. Hrstka,
J.C. Lee,
Y. Sunada,
O. Ibraghimov-Beskrovnaya, and
K.P. Campbell.
1997.
Dystroglycan is essential for early embryonic development: disruption of Reichert's membrane in Dag1-null mice.
Hum. Mol. Genet.
6:
831-841
|
59. | Xu, H., X.R. Wu, U.M. Wewer, and E. Engvall. 1994. Murine muscular dystrophy caused by a mutation in the laminin alpha 2 (Lama2) gene. Nat. Genet. 8: 297-302 |
60. | Yaffe, D., and O. Saxel. 1977. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature. 270: 725-727 |
61. | Yamada, K.M., and S. Miyamoto. 1995. Integrin transmembrane signaling and cytoskeletal control. Curr. Opin. Cell. Biol. 7: 681-689 |
62. | Yarden, Y., and J. Schlessinger. 1987. Epidermal growth factor induces rapid, reversible aggregation of the purified epidermal growth factor receptor. Biochemistry. 26: 1443-1451 |
63. |
Yurchenco, P.D., and
Y.S. Cheng.
1993.
Self-assembly and calcium-binding
sites in laminin. A three-arm interaction model.
J. Biol. Chem.
268:
17286-17299
|
64. | Yurchenco, P.D., and J.J. O'Rear. 1994. Basement membrane assembly. In Methods in Enzymology. Vol. 245. E. Ruoslahti and E. Engvall, editors. Academic Press, New York. 489-518. |
65. | Yurchenco, P.D., Y.S. Cheng, and H. Colognato. 1992. Laminin forms an independent network in basement membranes [published erratum appears in J. Cell Biol. 1992. 118:493]. J. Cell Biol. 117: 1119-1133 [Abstract]. |
66. |
Yurchenco, P.D.,
E.C. Tsilibary,
A.S. Charonis, and
H. Furthmayr.
1985.
Laminin polymerization in vitro. Evidence for a two-step assembly with
domain specificity.
J. Biol. Chem.
260:
7636-7644
|