* Department of Immunology and Oncology, Centro Nacional de Biotecnología, CSIC/UAM, Campus de Cantoblanco, E-28049
Madrid, Spain; Servicio de Inmunología, Hospital de la Princesa, Universidad Autónoma de Madrid, Diego de León 62, E-28006 Madrid, Spain; and § Serono Pharmaceutical Research Institute, Plan-les-Ouates, Geneva CH-1228, Switzerland
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Abstract |
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Chemokines are a family of proinflammatory
cytokines that attract and activate specific types of leukocytes. Chemokines mediate their effects via interaction with seven transmembrane G protein-coupled receptors (GPCR). Using CCR5-transfected HEK-293
cells, we show that both the CCR5 ligand, RANTES, as
well as its derivative, aminooxypentane (AOP)-
RANTES, trigger immediate responses such as Ca2+
influx, receptor dimerization, tyrosine phosphorylation,
and Gi as well as JAK/STAT association to the receptor. In contrast to RANTES, (AOP)-RANTES is unable to trigger late responses, as measured by the
association of focal adhesion kinase (FAK) to the
chemokine receptor complex, impaired cell polarization required for migration, or chemotaxis. The results
are discussed in the context of the dissociation of the
late signals, provoked by the chemokines required for
cell migration, from early signals.
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Introduction |
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RANTES is a member of the continuously growing
family of chemoattractant cytokines (chemokines).
The members of this group of low molecular
weight proinflammatory cytokines are characterized by
their ability to induce migration and activation of specific leukocyte subsets (Schall, 1994; Schall and Bacon, 1994
;
Baggiolini, 1998
). There are two major chemokine subfamilies, the CXC or
chemokines (in which the two cysteines nearest the NH2 terminus are separated by a single
amino acid) and the CC or
chemokines (in which these
two cysteines are adjacent). The structural similarities, chromosomal location, and overall specificity for different
leukocyte populations concur in these families (Baggiolini
et al., 1994
). Two exceptions to the CC/CXC classification
rule are as follows: lymphotactin, a chemokine lacking one
of the two conserved cysteines (Kelner et al., 1994
); and
fractalkine, an integral membrane protein with a chemokine domain in its NH2 terminus (Bazan et al., 1997
).
RANTES is a CC chemokine, and interacts with several
chemokine receptors (CCR1, CCR3, CCR4, and CCR5)
(Rollins, 1997). It is a potent chemoattractant for CD4+
and CD8+ lymphocytes, as well as for monocytes, NK
cells, and eosinophils (Schall et al., 1990
; Taub et al., 1995
;
Ugoccioni et al., 1995). RANTES can prevent infection
by macrophage-tropic HIV-1 strains (Cocchi et al., 1995
;
Alkhatib et al., 1996
), and its overexpression has been
demonstrated in various chronic pathologies. For example, in experimental autoimmune encephalomyelitis, RANTES
overexpression is followed by the appearance of infiltrating cells in the central nervous system (Godiska et al.,
1995
). Furthermore, in asthmalike inflammation models,
T cell and eosinophil accumulation in the lungs of sensitized mice are associated with RANTES and eotaxin expression (Gonzalo et al., 1996
; Jia et al., 1996
). Due to the possible relevance of this chemokine in diverse pathologies, therapy based on administration of chemokine receptor antagonists is a strategy for many of these diseases
(Baggiolini and Moser, 1997
). Hence, RANTES derivatives have been developed that lack chemotactic and
leukocyte-activating properties, and act as functional antagonists (Simmons et al., 1997
). This is the case for
(AOP)-RANTES, a RANTES analogue lacking the first
eight NH2-terminal amino acids (Arenzana-Seisdedos et
al., 1996
). This analogue binds to several CC chemokine
receptors (CCR1, CCR3, CCR4, and CCR5), but does not
induce chemotaxis or enzyme release, thus acting as a
RANTES antagonist (Gong et al., 1996
). In addition, (AOP)-
RANTES is a potent inhibitor of macrophage-tropic HIV-1
strains that use CCR5 as a coreceptor (Arenzana-Seisdedos et al., 1996
; Simmons et al., 1997
). (AOP)-RANTES
drives the internalization of CCR5 in recombinant cell
lines and in primary cells (Mack et al., 1998
).
Chemokines activate leukocytes through interaction
with single-chain seven transmembrane G protein-coupled receptors (GPCR)1 (Murphy, 1994) that are grouped
in two families: CC and CXC receptors, depending on the
chemokines they bind (Murphy, 1994
). To date, nine CC
and five CXC receptors have been reported, as have orphan receptors whose ligands remain to be elucidated. The
extracellular loops of these receptors act in concert to bind
the chemokine ligand, whereas different intracellular regions are involved in transducing the chemokine signal.
Chemokine receptors are generally coupled to G
i proteins (Bokoch, 1995
), rendering cellular responses pertussis toxin (PTX)-sensitive, although there are several examples of chemokine receptors that couple to both PTX-insensitive or -sensitive G proteins, as is the case of the C5a receptor (Amatruda et al., 1993
). Ligand binding triggers a
number of biochemical pathways (Ward et al., 1998
) that
lead to changes in intracellular cAMP levels (Ahmed et
al., 1995
); phospholipase activation (Bacon et al., 1995a
);
increases in tyrosine phosphorylation (Bacon et al., 1995b
),
including the Src substrates focal adhesion kinase (FAK)
(p125 FAK) and ZAP-70 (Bacon et al., 1996
); increased association of Src family kinases with p21ras (Worthen et al.,
1994
); and MAPK cascade activation (Jones et al., 1995
;
Ganju et al., 1998
).
We have recently demonstrated that the initiation of
chemokine signaling through the CCR2 chemokine receptor involves ligand-triggered receptor association to members of the Janus associated kinase (JAK) family of tyrosine kinases (Mellado et al., 1998), as is the case for the
cytokine receptors (O'Shea, 1997
). Thus, MCP-1 association to the CCR2 chemokine receptor induces CCR2
dimerization (Rodríguez-Frade et al., 1999
) and activation
of the JAK2/STAT3 signaling pathway (Mellado et al.,
1998
). RANTES and other chemokines implicated in regulating T cell functions, such as MIP-1
, also rapidly activate some of the STAT family transcription factors (Wong
and Fish, 1998
), suggesting similar dimerization-triggered responses.
Here we analyze early RANTES and (AOP)-RANTES-activated signaling events following binding to CCR5. Both RANTES and its modified form, (AOP)-RANTES, trigger tyrosine phosphorylation and activation of the JAK1/STAT5 pathway as well as promoting dimerization and tyrosine phosphorylation of the CCR5 receptor. While both promote Ca2+ influx, only RANTES induces sustained cell polarization and chemotaxis, indicating the activation of different signaling events. These data indicate that JAK/STAT pathway activation is a general mechanism for chemokine receptor activity and suggest that receptor dimerization and internalization may be key features in chemokine-induced signaling.
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Materials and Methods |
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Biological Materials
HEK-293 cells (ATCC TIB202) were obtained from the American Type
Culture Collection, and CCR5-transfected HEK-293 cells were kindly donated by Dr. J. Gutierrez (Dept. of Immunology and Oncology, Centro
Nacional de Biotecnología, Madrid, Spain). Antibodies used include rabbit anti-JAK1 and anti-JAK2 (Upstate Biotechnology, Inc.), anti-Gi and
anti-STAT5 (Santa Cruz Biotechnology); monoclonal anti-PTyr (4G10;
Upstate Biotechnology Inc.), anti-PTyr (PY20) (Transduction Laboratories), and anti-
2-microglobulin (PharMingen). Anti-CCR5 mAb, CCR5-02, CCR5-03, and anti-CXCR4 mAb were generated in our laboratory as
described (Mellado et al., 1997
). EGF-stimulated A-431 cell lysates were
obtained from Upstate Biotechnology Inc. and recombinant human
RANTES and SDF-1
were from Peprotech Inc.
Flow Cytometry Analysis
Cells were centrifuged (250 g, 10 min, room temperature), plated in V-bottom 96-well plates (2.5 × 105 cells/well), and incubated with 50 µl/ well biotin-labeled mAb (5 µg/ml, 60 min, 4°C). Cells were washed twice in PBS with 2% BSA and 2% FCS, and centrifuged (250 g, 5 min, 4°C). FITC-labeled streptavidin (Southern Biotechnologies Associates, Inc.) was added, incubated (30 min, 4°C) and plates were washed twice. Cell-bound fluorescence was determined in a Profile XL flow cytometer at 525 nm (Coulter Electronics).
Calcium Determination
Changes in intracellular calcium concentration were monitored using a
fluorescent probe (Fluo-3AM; Calbiochem). Cells (2.5 × 106 cells/ml),
were resuspended in RPMI containing 10% FCS, 10 mM Hepes, and incubated with 10 µl/106 cells of Fluo-3AM (300 µM in DMSO, 15 min, 37°C)
(Frade et al., 1997a). After incubation, cells were washed, resuspended in
complete medium containing 2 mM CaCl2, and maintained at 4°C until
just before chemokine addition to minimize membrane trafficking and to
eliminate spontaneous Ca2+ entry. Calcium mobilization in response to
10 nM RANTES or (AOP)-RANTES and 20 nM SDF-1
was determined
at 37°C in an EPICS XL flow cytometer at 525 nm. It includes background
level stabilization and determination of the probe loading level for each
sample. Only samples with a similar load, as assessed by ionophore-induced Ca2+ mobilization, were used (5 µg/ml ionomycin; Sigma Chemical Co.).
Cell Migration
CCR5-transfected HEK-293 cells (0.5 × 106 cells/ml) were starved for 120 min at 37°C, 5% CO2 in RPMI 1640 containing 0.1% BSA. Cells (0.25 × 106 cells in 0.1 ml) were placed in the upper well of 24-well, 8-µm pore size
transmigration chambers (Transwell; Costar Corp.) and RANTES or
(AOP)-RANTES (diluted in 0.6 ml RPMI containing 0.25% BSA) were
added to the lower well. Plates were incubated (240 min, 37°C, 5% CO2)
and the cells that migrated to the lower chamber were counted, as described (Frade et al., 1997a).
Immunoprecipitation, SDS-PAGE, and Western Blot Analysis
RANTES- or (AOP)-RANTES-stimulated cells (20 × 106) were lysed in
a detergent buffer (20 mM triethanolamine, pH 8.0, 300 mM NaCl, 2 mM
EDTA, 20% glycerol, 1% digitonin, with 10 µM sodium orthovanadate,
10 µg/ml leupeptin, and 10 µg/ml aprotinin) for 30 min at 4°C with continuous rocking and then centrifuged (15,000 g, 15 min). Immunoprecipitations were performed essentially as described earlier (Mellado et al.,
1997). Protein extracts precleared by incubation with 20 µg of anti-mouse
IgM-agarose (Sigma Chemical Co.) or protein A-Sepharose (60 min, 4°C)
were centrifuged (15,000 g, 1 min), immunoprecipitated with the appropriate antibody (5 µg/sample, 120 min, 4°C), followed by anti-mouse IgM-agarose or by protein A-Sepharose if the first antibody was derived from rabbit serum. Immunoprecipitates or protein extracts were separated in
SDS-PAGE and transferred to nitrocellulose membranes. Western blot
analysis was performed as described (Mellado et al., 1997
), using 2% BSA
in TBS as blocking agent for the antiphosphotyrosine analyses. When
stripping was required, membranes were incubated for 60 min at 60°C
with 62.5 mM Tris-HCl, pH 7.8, containing 2% SDS and 0.5%
-mercaptoethanol. After washing with 0.1% Tween 20 in TBS for 2 h, membranes
were reblocked, reprobed with the appropriate antibody, and developed
as above. In all cases, protein loading was carefully controlled using a protein detection kit (Pierce Chemical Co.) and, when necessary, by reprobing the membrane with the immunoprecipitating antibody.
Receptor Cross-linking Assays
Serum starved CCR5 transfected HEK-293 cells (20 × 106) were stimulated with 10 nM RANTES, 10 nM (AOP)-RANTES, or 20 nM SDF-1 for 1 min at 37°C. The reaction was terminated by addition of 1 ml of cold
PBS and centrifugation (30 s, 15,000 g). After washing twice with cold
PBS, 10 µl of 100 mM disuccinimidyl suberate (DSS; Pierce Chemical
Co.) was added for 10 min at 4°C with continuous rocking. The reaction
was terminated by adding 1 ml of cold PBS and washing three times. The
pellet was lysed for 60 min and immunoprecipitated as above.
Immunofluorescence Microscopy: Polarization of Blast Cells
Immunofluorescence experiments were performed essentially as described (Nieto et al., 1997). Briefly, 2 × 106 T lymphoblasts in 500 µl complete medium were allowed to adhere to fibronectin (FN)-coated coverslips in 24-well plates (Costar Corp.). Cytokines and chemokines (10 ng/ml)
were added, and cells were incubated (37°C, 5% CO2). After 30 min, cells
were fixed with 3.7% formaldehyde in PBS for 10 min at room temperature, and stained with specific mAb plus a FITC-labeled rabbit anti- mouse IgG F(ab')2 (Pierce Chemical Co.). The proportion of CCR5 polarization was calculated by direct cell count (n = 400-500) of 10 random
fields for each condition using a photomicroscope with 60× oil immersion
objectives (Labophot-2; Nikon Inc.). Preparations were photographed on
Ektachrome 400 film. Images were acquired with a high performance
CCD camera (Cohu) coupled to the microscope and connected to a workstation (model Q550CW; Leica Imaging Systems, Ltd.). Images were visualized, processed, and stored using QFISH software (version V1.01; Leica
Imaging Systems, Ltd.) and printed on a color printer (Phaser 440; Tektronix Inc.).
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Results |
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RANTES and (AOP)-RANTES Induce Functional Responses in CCR5-transfected HEK-293 Cells
We developed anti-human CCR5-specific mAb using synthetic peptides corresponding to the NH2-terminal domain of this receptor (amino acids 13-28), a CCR5-specific sequence not present in other chemokine receptors. These mAbs recognize the CCR5 in human PBMC (not shown) and in CCR5 stably transfected HEK-293 cells, in flow cytometry (Fig. 1 A), as well as in Western blot, and immunoprecipitation analysis (Fig. 1 B). No binding is observed in mock- or CCR2-transfected HEK-293 cells, or in any other cell tested that does not express CCR5.
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In response to RANTES or (AOP)-RANTES, these
CCR5-transfected HEK-293 cells mobilize calcium (Fig. 2
A) and are desensitized to a second stimulation. However,
only RANTES triggers migration in these cells (Fig. 2 B).
PTX treatment abrogates both calcium release and cell
migration in response to RANTES and (AOP)-RANTES (Fig. 2 A), whereas no effect was observed following incubation with cholera toxin (not shown). This is consistent
with other studies showing that RANTES downstream signals in other cell lines and T cells are coupled to PTX-sensitive G proteins (Bacon et al., 1995b), and with coupling
of other chemokine receptors to Gi in transfected HEK-293 cells (Aragay et al., 1998
; Mellado et al., 1998
), indicating the utility of this cell line in the study of signaling
through GPCR.
|
We have described the ability of RANTES to polarize
peripheral blood T cells, such that the CCR5 receptor migrates to the cell's leading edge while the ICAM-1, ICAM-3/
CD43, CD44 molecules localize at the uropod (Nieto et al.,
1997). Therefore, we tested the ability of both of these
ligands to induce polarization in peripheral T cell blasts.
Time-course studies of ICAM-3 redistribution to the cell
uropod of FN-adhered T lymphoblasts showed that RANTES induced ICAM-3 redistribution in a significant proportion
of the cells as early as 15 min after stimulation. Maximum redistribution, seen at 30 min, persisted until at least
90 min of incubation with the chemokine. In contrast,
(AOP)-RANTES-induced ICAM-3 polarization showed
a much slower, weaker response, with a peak at 30 min
that vanished thereafter (Fig. 3 A).
|
We next studied the effects of these ligands on the polarization of CCR5 to the advancing front of migrating T
lymphoblasts, a phenomenon that may be involved in the
chemotaxis mechanism. We found that whereas RANTES
triggered redistribution of CCR5 molecules to the leading
edge of the cells, membrane CCR5 expression was not detectable in (AOP)-RANTES-stimulated polarized T lymphoblasts bearing ICAM-3 redistributed to the uropod
(Fig. 3 B). The downregulation of the (AOP)-RANTES-
triggered chemokine receptor results in a net inhibition of
CCR5 redistribution to the leading edge of T lymphoblasts
stimulated with this chemokine derivative, compared to
those treated with RANTES (Fig. 3 C). This concurs with
previous observations showing that (AOP)-RANTES induces CCR5 downregulation by blocking receptor recycling (Mack et al., 1998).
RANTES and (AOP)-RANTES Induce Tyrosine Phosphorylation of the CCR5 Receptor and Association of the JAK1/STAT5 Complex
When CCR5-transfected HEK-293 cells were stimulated
with RANTES or (AOP)-RANTES, a 38-kD protein
phosphorylated in tyrosine residues was initially identified
as the CCR5 receptor by immunoblot (not shown). Thereafter, lysates from transfected HEK-293 cells were immunoprecipitated with anti-CCR5, and Western blots developed with anti-PTyr antibodies (Fig. 4). An increase in
CCR5 receptor phosphorylation in tyrosines is seen as
early as 60 s after stimulation. At this time no differences
were observed following treatment with either stimulus,
indicating that (AOP)-RANTES not only binds to CCR5,
but also signals through this receptor. The early, weak tyrosine phosphorylation level may occur because only one
tyrosine (Tyr 126 in the DRY motif) was being phosphorylated, analogous to the case of Tyr 139 in the CCR2 receptor (Mellado et al., 1998). Similar results are observed in
the inverse experiment, in which anti-PTyr antibody
immunoprecipitates are analyzed in Western blot with
anti-CCR5 receptor antibody (not shown). Again, tyrosine phosphorylation is detected in CCR5 after stimulation
with either RANTES or (AOP)-RANTES. Nevertheless,
significant differences are observed in tyrosine phosphorylated CCR5 after longer stimulation periods. Whereas
CCR5 phosphorylation persists in RANTES-treated cells
after 15 min of treatment, this is not the case after (AOP)- RANTES stimulation, reinforcing the observation that
there are differences in the signaling pathways activated
by these chemokines.
|
To ascertain which kinase is responsible for the rapid
CCR5 chemokine receptor phosphorylation, CCR5-transfected HEK-293 cells were stimulated with RANTES or
(AOP)-RANTES, and cell lysates immunoprecipitated
with anti-CCR5, or with anti-2-microglobulin as an isotype-matched antibody control. The use of a specific anti-JAK1 antibody identified a 130-kD protein in the anti-CCR5 immunoprecipitates (Fig. 5 A). Furthermore, the
JAK1 tyrosine kinase is phosphorylated on tyrosine residues after stimulation with either ligand, since anti-PTyr
immunoprecipitates can be developed with anti-JAK1 antibodies in Western blot (Fig. 5 B). JAK1 association to
the CCR5 receptor takes place as early as 30 s after
RANTES or (AOP)-RANTES stimulation (Fig. 5 A).
Small amounts of JAK1 were also found associated to the
CCR5 receptor in the absence of added ligand, consistent
with receptor phosphorylation in the absence of exogenous ligand in CCR5-transfected HEK-293 cells (Fig. 4, A
and B).
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Neither immunoprecipitation of cell lysates with isotype-
matched control antibodies to other cell proteins such as
2-microglobulin nor immunoprecipitation in mock-transfected HEK-293 cells (not shown) revealed the presence
of JAK1 (Fig. 5 A). This rules out nonspecific protein association to membrane components under these experimental conditions. The rapid association of JAK1 to the CCR5 receptor triggered by these two chemokines, but
not by others tested (MCP-1 and SDF-1
), suggests a role
for this tyrosine kinase in early receptor phosphorylation
following ligand stimulation. Interestingly, while MCP-1
promotes JAK2 association to the receptor in CCR2-transfected HEK-293 cells, neither JAK2 nor JAK3 associates to CCR5 after RANTES or (AOP)-RANTES activation of CCR5-transfected HEK-293 cells (data not shown).
To identify the downstream signaling pathway activated by the JAK1 kinase, we tested its association to members of the STAT transcription factor family. We analyzed JAK1 kinase-activated STAT transcription factors in anti-CCR5 immunoprecipitates, and found that STAT5 associates to the receptor complex in response to both ligands (Fig. 5 C). The association of STAT5 correlates in time with JAK1 phosphorylation and binding. Furthermore, the precipitated STAT5 is phosphorylated on tyrosine residues, showing it is in the activated state (Fig. 5 D). Immunoprecipitates of RANTES-stimulated cell lysates with isotype-matched control antibodies do not contain STAT5. These results were further validated by the reverse experiments, in which anti-STAT5 antibodies were used for immunoprecipitation and Western blots developed with either anti-PTyr or anti-CCR5 antibodies (not shown). Therefore, binding of both RANTES and (AOP)-RANTES to the CCR5 receptor induces receptor and JAK1 phosphorylation and STAT5 binding. Independent of the fact that other STAT transcriptional factors may be implicated in CCR5-mediated signaling, our data show that no major differences are observed in RANTES or (AOP)-RANTES activation of the JAK/STAT pathway.
RANTES and (AOP)-RANTES Induce CCR5 Dimerization
Similar to growth factor-induced dimerization of tyrosine
kinase receptors, some GPCR, including chemokine receptors like CCR2 and CXCR4, undergo ligand-induced
dimerization (Hebert et al., 1996; Rodríguez-Frade et
al., 1999
; Vila-Coro, A.J., J.M. Rodríguez-Frade, A. Martín de Ana, M.C. Moreno-Ortiz, C. Martínez-A., and
M. Mellado, manuscript submitted for publication). We
have now tested whether RANTES and (AOP)-RANTES
trigger CCR5 dimerization. DSS-mediated cross-linking in
CCR5-transfected HEK-293 cells was carried out after
RANTES or (AOP)-RANTES stimulation. In CCR5-transfected HEK-293 cells, but not in mock-transfected
cells, a high molecular mass receptor species (76 kD) was
observed following both stimuli. This band corresponds to
the expected molecular mass of two CCR5 molecules, as
assessed by immunoprecipitation with anti-CCR5 antibodies and Western blot developed with anti-CCR5 antibodies (Fig. 6 A). When CCR5-transfected HEK-293 cells
were stimulated with SDF-1
, no CCR5 dimerization was
observed under these experimental conditions (Fig. 6 A),
despite the fact that HEK-293 cells express a functional CXCR4 receptor as determined by flow cytometry and
SDF-1
-induced calcium mobilization assays (Fig. 6 B).
When the same experiment was done using anti-CXCR4
mAb for immunoprecipitation and Western blot, a high
molecular mass species (84 kD) of the CXCR4 receptor is
observed only after SDF-1
, but not after RANTES or
(AOP)-RANTES stimulation (Fig. 6 B).
|
All together, these data indicate specific ligand-induced
dimerization, as SDF-1 is not able to induce CCR5
dimerization, while RANTES or (AOP)-RANTES are not
able to induce CXCR4 dimers. In addition, association of
CCR5 with other membrane components, including other
chemokine receptors, can be discarded under these experimental conditions, as cells treated with SDF-1
render
only CXCR4 dimers, and those treated with RANTES and
(AOP)-RANTES render only CCR5 dimers. High molecular mass species containing CCR5 and CXCR4 simultaneously are not detected, as shown when cells are stimulated with SDF-1
or RANTES and (AOP)-RANTES,
and when immunoprecipitation and Western blot are performed using anti-CCR5 or anti-CXCR4 antibodies, respectively. Both RANTES and (AOP)-RANTES, like
other chemokines and similar to members of the large cytokine family, can trigger early signaling events such as receptor dimerization, followed by recruitment of tyrosine
kinases that phosphorylate both the receptor and the
STAT transcription factors.
Gi and p125FAK Association to CCR5 Differ after
RANTES or (AOP)-RANTES Stimulation
The PTX dependence of RANTES- and (AOP)-
RANTES-triggered Ca2+ mobilization indicates Gi involvement in this process. It has been demonstrated recently that chemokine receptor-mediated chemotaxis is
triggered by the
subunit of G
i (Neptune and Bourne,
1997
). In MCP-1-mediated chemotaxis, we have shown
that G
i associates rapidly to the CCR2 receptor, releasing
, which is responsible for activating migration and
cell polarization. Thus, we investigated whether the differences in chemotaxis triggering reflect differences in
RANTES- and (AOP)-RANTES-mediated G
i-CCR5
receptor association.
CCR5- or mock-transfected HEK-293 cells were stimulated with RANTES or (AOP)-RANTES, immunoprecipitated with anti-CCR5 or isotype-matched control
antibodies, followed by a Western blot with anti-Gi antibodies. After both RANTES and (AOP)-RANTES stimulation, G
i associated to CCR5 (Fig. 7 A). Whereas association persisted for longer than 15 min when cells were
RANTES-stimulated, G
i is dissociated from the receptor
after 5 min of (AOP)-RANTES treatment (Fig. 7 A). The
CCR5 expression level was controlled in both immunoprecipitates by stripping and reblotting membranes with anti-CCR5 antibody (Fig. 7 A). These data concur with the
similar Ca2+ mobilization promoted by both of these
ligands (Fig. 2 A), as G
i association to CCR5 is unaltered
at the times employed for these experiments (1-3 min).
Rapid G
i dissociation from the CCR5, induced only by
(AOP)-RANTES, implies a role for Gi in later chemokine-triggered events such as chemotaxis. This result concurs with data showing the importance of
release from
Gi in chemotaxis (Neptune and Bourne, 1997
).
|
The activation of p125FAK and the kinase ZAP-70 has
been described in T cells after RANTES activation (Bacon et al., 1996). Therefore, we tested whether p125FAK
activation coincides with its association to the CCR5.
CCR5-transfected HEK-293 cells were stimulated with
10 nM RANTES or (AOP)-RANTES, cell lysates were
immunoprecipitated with anti-p125FAK or an isotype-matched control antibody and analyzed in Western blot
for the presence of CCR5. RANTES, but not (AOP)-
RANTES, promotes the association of this kinase to the
receptor, reaching maximum binding after 15 min (Fig. 7
B). Even in the absence of ligand, residual association
between these two proteins was observed. The lack of
p125FAK association and the rapid G
i dissociation from
the CCR5 receptor in the case of (AOP)-RANTES suggest that association of these proteins with the receptor is
crucial in triggering the signaling pathways that lead to the
different effects promoted by these two chemokines.
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Discussion |
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Interest is rapidly growing in a broad view of the chemokine function, not only because of significance in HIV infection, but also because chemokines and their receptors
are expressed by a wide variety of nonhematopoietic cells
(Horuk et al., 1997). Because of the relevance of chemokines in numerous pathologies, it appears of the utmost
importance to devise therapies based on the administration of chemokines that lack chemotactic and leukocyte-activating properties, but are able to act as receptor antagonists. These are the basic characteristics of (AOP)-
RANTES, a RANTES analogue that has been chemically
modified at its NH2 terminus, although very little is known
of the mechanism underlying its action.
We have generated anti-CCR5 mAbs and used them to
characterize the signaling pathways activated by RANTES
and (AOP)-RANTES. The results show that ligand-induced CCR5 dimerization occurs when these chemokines signal target cells for activation. In the response to
cytokines, receptor dimerization triggers a pathway involving tyrosine kinases and transcriptional factors. Only
the JAK1 tyrosine kinase responds to RANTES and to
(AOP)-RANTES by rapidly associating to CCR5 and is
phosphorylated as soon as 30 s after binding, indicating
that JAK1 activation is virtually simultaneous with its association to CCR5. In the cytokine receptors, activation
and association of JAK kinase to the receptor creates docking sites for SH2-containing proteins such as STAT,
leading to their phosphorylation, followed by activation of
gene transcription (Ihle, 1996). After binding of either of
the chemokine ligands studied here, STAT5 is also associated to the CCR5, in accordance with the role of the JAK
kinases in transducing signals from hematopoietic growth
factor receptors (O'Shea, 1997
). Our data concur with a recent report showing rapid activation of certain STAT
transcription factors in T cells after RANTES and MIP-1
stimulation, although in that case association to the CCR5
receptor was not analyzed (Wong and Fish, 1998
). Together, these results indicate that chemokine-mediated activation of GPCR leads to signal transduction, which invokes intracellular phosphorylation intermediates used by
other cytokine receptors. These earlier events are equally
activated by both RANTES and (AOP)-RANTES.
Both RANTES and (AOP)-RANTES induce CCR5
dimerization, a phenomenon that requires specific interaction between a given chemokine and its respective receptor. When experiments were performed using SDF-1 as
a ligand neither CCR5 dimers nor CCR5-CXCR4 heterodimers were observed, whereas CXCR4 dimers are
present in anti-CXCR4 immunoprecipitates. Direct interaction between two CCR5 molecules has also been demonstrated by Benkirane et al. (1997)
, even in the absence
of ligand stimulation. The functional significance of dimerization was suggested by Hebert et al. (1996)
using the epitope tagging approach, showing that agonist stimulation of the
2-adrenergic receptor stabilized the dimeric
state of the receptor. The present data indicate that although dimerization is a primary event following ligand
interaction with the chemokine receptor, it does not
necessarily initiate identical transduction events. (AOP)-
RANTES thus triggers Ca2+ mobilization as does RANTES,
but promotes neither cell migration nor CCR5 redistribution to the leading edge in cell polarization. The rapid G
i
association to CCR5 induced by both ligands explains the
similar Ca2+ mobilization response and lack of response in
PTX-pretreated cells.
It has been shown for a number of GPCR that different
ligands of a given receptor can induce different responses.
This is the case for CXCR2, from which IL-8 and NAP-2
elicit different responses, although they bind with equivalent affinity to this receptor (Ben-Baruch et al., 1997). It is
feasible that RANTES and (AOP)-RANTES induce distinct conformational changes in the CCR5. In fact, it has
been shown that different structural requirements are
needed in GPCR to activate different pathways (Hunyady
et al., 1995
; Arai et al., 1997a
). In the case of angiotensin II receptor changes have been described in internalization
kinetics that do not alter other functional responses (Hunyady et al., 1995
).
Whereas Gi association to CCR5 persists as long as 15 min after RANTES treatment, it dissociates from CCR5
by 5 min after (AOP)-RANTES stimulation. This variance
must give rise to a clear difference in the availability of
free, active
subunits by affecting some ligand-induced
responses. In fact, Gi
subunit release is known to be
important in chemokine receptor-mediated chemotaxis
(Arai et al., 1997b
; Neptune and Bourne, 1997
). Another conspicuous difference is the lack of late tyrosine phosphorylation in CCR5 after (AOP)-RANTES treatment, in
contrast to the continuous tyrosine phosphorylation of
CCR5 observed following RANTES induction. This may
occur because of tyrosine phosphorylation mediated by other tyrosine kinases. The activation of both p125FAK
and ZAP-70 has been described after RANTES stimulation (Bacon et al., 1996
). Here we demonstrate association
of p125FAK, which is related to cytoskeletal proteins and
migration phenomena (Sieg et al., 1998
), to the receptor
following RANTES but not (AOP)-RANTES stimulation.
The failure of (AOP)-RANTES to maintain Gi association
for long (15 min) periods concurs with the lack of association and activation of p125FAK and the inactivation of subsequent events. Both activation of Gi and p125FAK, as well
as chemotaxis, occur after RANTES activation, but are
absent when cells were treated with (AOP)-RANTES, indicating a possible relationship between these events.
Chemotaxis involves several phenomena including:
changes in cell shape, integrin affinity, and integrin recycling at the cell's leading edge (Condeelis, 1993; Lawson
and Maxfield, 1995
). These events appear to be mediated
by G protein activation, as well as phosphorylation signals
through chemokine receptors (Bokoch, 1995
). In fibroblasts and smooth muscle cells, the regulation of intracellular FAK and its interaction with the cytoskeletal proteins
-actinin, talin, and vinculin have also been given
much attention with respect to cell polarization and migration (Clark and Brugge, 1995
; Miyamoto et al., 1995
). As a
consequence of RANTES stimulation, T cells undergo a
polarization process in which the CCR5 receptor redistributes to the leading cell edge and p125FAK associates to the
receptor. Both events, implicated in the late response to
chemokines, are required for chemotaxis and are triggered
by RANTES. Early (AOP)-RANTES-induced signaling
events, such as Ca2+ mobilization, result in weak, transient
cell polarization, and redistribution of the ICAM-3 adhesion receptor to the T lymphocyte uropod. This antagonist
does not mediate cellular chemotaxis. Recycling of CCR5
molecules to the cell membrane, which probably occurs at
the advancing front of the cell and is inhibited by (AOP)-
RANTES (Mack et al., 1998
), appears to be critical for the
mechanism of directional cell migration towards a chemokine gradient. This is further supported by our data showing the failure of CCR5 to cluster at the leading edge of
(AOP)-RANTES-stimulated cells. Therefore, these results suggest that during the chemotaxis process early signals induced by chemokines through receptor dimerization may not be sufficient to induce cell migration. Signals
triggered by sustained association of the G protein to the
chemokine receptor may also regulate other events necessary for the chemotactic response, such as integrin affinity
or actin polymerization at the leading cell edge. Our data
also support the idea that p125FAK kinase association to
the receptor is not involved in ligand-induced CCR5
internalization, since (AOP)-RANTES is as efficient as
RANTES in promoting internalization (Mack et al., 1998
),
without inducing p125FAK association.
The data presented here can be incorporated in a model in which signaling through chemokine receptors directly involves early signals that occur in the first few minutes after ligand binding, including receptor dimerization; association and activation of JAK tyrosine kinases, and activation of STAT transcriptional factors, as well as late signals such as cell polarization, activation, and association of p125FAK kinase to the chemokine receptor (Fig. 8). The fact that chemokine receptors activate the JAK/STAT pathway and that dimerization may be a general mechanism for chemokine activity adds a new perspective to understanding how the multiplicity of chemokine functions are achieved. Furthermore, it suggests an interesting new objective for therapeutic intervention in chemokine-associated pathologies, including inflammation and AIDS.
|
Internalization of chemokine receptors is a phenomenon that can be dissociated from their role as HIV-1 coreceptors (Aramori et al., 1997; Howard et al., 1998
). Although the ability of the coreceptor to internalize is not
required for HIV entry, it contributes to the HIV suppressive effect of CC and CXC chemokines (Amara et al.,
1997
). (AOP)-RANTES is a potent inhibitor of HIV-1 infectivity, an effect that is suggested because it enhanced
CCR5 internalization and inhibition of recycling (Mack
et al., 1998
). As internalization is an agonist-driven event,
(AOP)-RANTES as well as other NH2-terminal modified
chemokines should be able to activate their receptors. The
differences between RANTES- and (AOP)-RANTES-
induced internalization may be related to increased affinity of (AOP)-RANTES for the CCR5 receptor (Mack et
al., 1998
), although differences in the signaling pathways
activated by these two ligands have not been reported. Here we correlate the known effects of (AOP)-RANTES
with a signaling cascade and suggest that the dimerization
of chemokine receptors is an alternative mechanism for
chemokine-induced HIV-1 inhibition.
Receptor dimerization is also the mechanism through
which antibodies to the CCR2 NH2-terminal domain block
HIV-1 infection. In fact, the anti-CCR2 mAb CCR2-01
that blocks HIV-1 infection (Frade et al., 1997b), but not
MCP-1-induced responses (Frade et al., 1997a
), triggers
CCR2 dimerization (Mellado, M., manuscript in preparation). The concept that receptor dimerization is required for prevention of HIV-1 infection, besides including the
nonsignaling mechanism indicated by (AOP)-RANTES,
incorporates the model based on receptor internalization,
which does not take place in the absence of dimerization.
For both receptor desensitization and internalization, we
have shown the requirement for GRK2 association and
phosphorylation in serine/threonine residues of the COOH- terminal receptor domain. This in turn associates with
arrestin and clathrin, and undergoes internalization (Aragay
et al., 1998
). Dimerization is a necessary event for the formation of the receptor/GRK-2/arrestin/clathrin complex.
This model consolidates all available experimental evidence, and offers novel prospects for screening new drugs
that may prevent HIV-1 infection and/or inflammatory processes.
![]() |
Footnotes |
---|
Received for publication 25 August 1998 and in revised form 18 December 1998.
Address correspondence to Carlos Martínez-A., Department of Immunology and Oncology, Centro Nacional de Biotecnología, CSIC/UAM, Campus de Cantoblanco, E-28049 Madrid, Spain. Tel.: 34-91-585-4559. Fax: 34-91-372-0493. E-mail: cmartineza{at}cnb.uam.es
We thank Drs. R. Geha (Harvard Medical School, Boston, MA) and J.C. Gutierrez-Ramos (Millennium Pharmaceuticals, Boston, MA) for critical reading of the manuscript, Dr. J. Gutiérrez for CCR5 transfectants, L. Gómez for help with animals, M.C. Moreno and I. López for FACS® analysis, and C. Bastos and C. Mark for secretarial and editorial assistance, respectively.
This work was supported by grants from the Spanish Dirección General de Investigaciones Cientificas, European Union, the Spanish CICyT and the Regional Government of Madrid (CAM), and Pharmacia & Upjohn. The Department of Immunology and Oncology was founded and is supported by the Spanish Research Council (CSIC) and Pharmacia & Upjohn.
![]() |
Abbreviations used in this paper |
---|
DSS, disuccinimidyl suberate; FAK, focal adhesion kinase; FN, fibronectin; GPCR, G protein-coupled receptors; JAK, Janus associated kinase; PTX, pertussis toxin.
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