* Howard Hughes Medical Institute, The organization of the actin cytoskeleton
can be regulated by soluble factors that trigger signal
transduction events involving the Rho family of GTPases. Since adhesive interactions are also capable of organizing the actin-based cytoskeleton, we examined the
role of Cdc42-, Rac-, and Rho-dependent signaling
pathways in regulating the cytoskeleton during integrin-mediated adhesion and cell spreading using dominant-inhibitory mutants of these GTPases. When Rat1
cells initially adhere to the extracellular matrix protein
fibronectin, punctate focal complexes form at the cell
periphery. Concomitant with focal complex formation,
we observed some phosphorylation of the focal adhesion kinase (FAK) and Src, which occurred independently of Rho family GTPases. However, subsequent phosphorylation of FAK and paxillin occurs in a Rho-dependent manner. Moreover, we found Rho dependence of the assembly of large focal adhesions from
which actin stress fibers radiate. Initial adhesion to fibronectin also stimulates membrane ruffling; we show
that this ruffling is independent of Rho but is dependent on both Cdc42 and Rac. Furthermore, we observed that Cdc42 controls the integrin-dependent activation of extracellular signal-regulated kinase 2 and of
Akt, a kinase whose activity has been demonstrated to
be dependent on phosphatidylinositol (PI) 3-kinase.
Since Rac-dependent membrane ruffling can be stimulated by PI 3-kinase, it appears that Cdc42, PI 3-kinase,
and Rac lie on a distinct pathway that regulates adhesion-induced membrane ruffling. In contrast to the differential regulation of integrin-mediated signaling by
Cdc42, Rac, and Rho, we observed that all three GTPases regulate cell spreading, an event that may indirectly control cellular architecture. Therefore, several
separable signaling pathways regulated by different members of the Rho family of GTPases converge to
control adhesion-dependent changes in the organization of the cytoskeleton, changes that regulate cell morphology and behavior.
ADHESIVE interactions play an important role in regulating numerous cellular functions during the development and maintenance of an organism, and
alterations in these interactions can lead to numerous disorders. In tissue culture, cells adhere to the underlying extracellular matrix (ECM)1 via integrins, a family of cell
surface receptors, at discrete sites termed "focal adhesions" (Burridge and Chrzanowska-Wodnicka, 1996 Integrin engagement and subsequent clustering of these
receptors in focal adhesions leads to the generation of intracellular macromolecular complexes (Yamada and Miyamoto, 1995 The Rho family of GTPases appears poised to contribute to these integrin-mediated signals; in particular, signals
that control cytoskeletal organization involved in changes
in cell morphology. Rho family members such as Cdc42,
Rac1, and RhoA are part of the Ras superfamily of proteins that cycle between an active, GTP-bound state and
an inactive, GDP-bound state. Activated RhoA is capable of stimulating microfilament bundling in serum-starved
cells that are already adherent (Ridley and Hall, 1992 Considerably less is known about how the ECM acts as
an insoluble extracellular agent capable of inducing the assembly of these same structures when cells are plated on a
matrix. For instance, is the Rho family of GTPases involved in the generation of these morphological structures? Initial studies suggested that in Swiss 3T3 cells integrin engagement of the matrix alone was not sufficient to
induce the assembly of vinculin-containing focal adhesions; Rho must also be activated by a soluble factor
(Hotchin and Hall, 1995 Reagents
Antibodies to paxillin (No. P13520), phosphotyrosine (PY20), and Erk
(No. E17120) were purchased from Transduction Laboratories (Lexington, KY). The antibody to the c-Myc tag (9E10) was purchased from
Santa Cruz Biotechnology (Santa Cruz, CA), and the anti-hemagglutinin (HA) antibody (12CA5) was purchased from Boehringer Mannheim
Corp. (Indianapolis, IN). The polyclonal antiserum to the COOH-terminal of FAK was a gift from J.-L. Guan (Cornell University, Ithaca, NY).
The antibody to Src (phosphotyrosine 416) was a gift from A. Laudano
(University of New Hampshire, Durham, NH). The polyclonal antiserum
to Akt was provided by P. Tsichlis (Fox Chase Cancer Center, Philadelphia, PA). The anti-integrin Cell Culture
Rat1 lines expressing N17Rac1 or N19RhoA driven by a tetracycline-
repressible promoter were established as described (Qiu et al., 1995 Transient Transfection Analysis
Calcium phosphate transient transfection of Rat1 cells was performed essentially as described (Sambrook et al., 1989 Cell Preparation for Adhesion
The cell lines described above were washed in PBS and then starved in serum-free DME for 24 h. Starved cells were trypsinized for 2 min, washed
with DME containing 1 mg/ml soybean trypsin inhibitor and 0.1% BSA,
and then finally resuspended in DME for 30 min at 37°C before plating
the cells on polylysine- (0.5 mg/ml) or fibronectin-coated (10 µg/ml)
dishes or coverslips as previously described (Clark and Hynes, 1996 Immunofluorescence
Adherent cells were rinsed in PBS, fixed in 4% PFA in PBS, and then permeabilized with 0.5% NP-40 in PBS. Filamentous actin (F-actin) was
stained using rhodamine-labeled phalloidin and focal adhesions were
stained using an antibody to vinculin (Sigma Chemical Co., St. Louis,
MO) or to the integrin Cell Lysis, Immunoprecipitation, and Immunoblotting
Cells were washed twice in PBS and lysed in radioimmunoprecipitation
assay (RIPA) buffer (1% Triton X-100, 1% sodium deoxycholate, 0.1%
SDS, 150 mM NaCl, 10 mM Tris-HCl, pH 7.2, 1 mM EGTA, 1 mM PMSF,
500 µg/ml leupeptin, 1 mM sodium orthovanadate, 50 mM sodium fluoride) for 30 min on ice. The lysates were clarified by centrifugation at
16,000 g for 10 min at 4°C, and protein concentration was then determined
using a Pierce Micro BCA protein assay kit (Pierce Chemical Co., Rockford, IL). Immunoprecipitations were then performed using 50 µg (for
FAK) or 100 µg (for paxillin) of RIPA lysate, the appropriate antibody,
and protein A-Sepharose. Duplicate immunoprecipitates of FAK and
paxillin were immunoblotted with their respective antibodies to ensure
that equal amounts of these proteins were present. Total cell lysates were
prepared by adding one part 4× SDS sample buffer (8% SDS, 2% 2-mercaptoethanol, 266 mM Tris, pH 7.2, 40 mM EDTA) to three parts RIPA
lysate. The samples were then heated to 100°C for 5 min and run on SDS-polyacrylamide gels. Immunoblotting of the total cell lysates or immunoprecipitates was performed essentially as described (Clark and Brugge,
1993 Erk In Vitro Kinase Assay
Cells were washed twice in PBS and lysed in Erk lysis buffer (50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 50 mM NaF, 5 mM EDTA, 1% Triton X-100,
40 mM Akt In Vitro Kinase Assay
Cells were washed twice in PBS and lysed in Akt lysis buffer (20 mM Tris,
pH 7.5, 150 mM sodium chloride, 10% glycerol, 1% NP-40, 10 mM sodium
fluoride, 1 mM sodium orthovanadate, 500 µg/ml leupeptin, 2 mM aprotinin, and 1 mM PMSF). Akt was immunoprecipitated from 250 to 500 µg
of lysate with a polyclonal antibody to Akt or the HA tag antibody
(12CA5) and subjected to an in vitro kinase reaction using histone H2B as
an exogenous substrate as described (King et al., 1997 Adhesion and Spreading Assays
Serum-starved cells (500,000 cells/ml) suspended in 1 ml of DME as described above were plated on fibronectin-coated/BSA-blocked 35-mm
wells. To initiate adhesion, the cells were centrifuged for 30 s at 50 g and
allowed to adhere (10 min) or spread (20 min) at 37°C. Adherent cells
were washed twice with PBS and then fixed with 4% PFA in PBS for 15 min at room temperature. The fixed cells were then rinsed twice with water, stained with 0.1% crystal violet in water for 30 min at room temperature, and rinsed twice with water. Adhesion was quantitated by adding
10% acetic acid to the crystal violet-stained well and examining the solution in a spectrophotometer at 600 nm. Background staining was determined by staining cells that adhere to a BSA-coated well in the absence of
fibronectin. Spreading was quantitated by photographing the cells that adhere to fibronectin-coated wells after 20 min and counting the cells that
had spread.
Determination of F-Actin Content
F-actin content was examined essentially as described (Southwick et al.,
1989 Changes in Cell Morphology That Accompany Cell
Spreading on Fibronectin
When grown in culture, many cells form specialized adhesive structures that link them to the underlying matrix.
During the assembly of these structures, there are dramatic changes in cell morphology. To define the changes
in cell morphology that accompany cell adhesion and
spreading, serum-starved Rat1 fibroblasts were plated
onto fibronectin for various times and stained for focal adhesions and F-actin. Fig. 1 shows 15-min and 60-min time
points. Rat1 cells adhered rapidly to fibronectin, with
>90% of the cells adhering within 10 min. By 15 min, most
of the cells had begun to flatten, with large membranous
protrusions ("ruffles") appearing at the cell perimeter
(Fig. 1 a). In those cells that had initiated cell spreading,
punctate concentrations of vinculin were present at the perimeter in structures (Fig. 1 b) that resembled "point contacts" (Tawil et al., 1993 Department of Biology, Massachusetts Institute of
Technology, Cambridge, Massachusetts 02139; § Department of Cell Biology, Harvard Medical School, Boston, Massachusetts
02115; and
Onyx Pharmaceuticals, 3031 Research Drive, Richmond, California 94806
Abstract
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References
Introduction
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References
; Craig
and Johnson, 1996
; Jockusch and Rudiger, 1996
). Integrins then link the ECM to large bundles of intracellular microfilaments that form prominent "stress fibers" in many
cell types. When cells are plated on an ECM-coated surface, complex series of events are set in motion that contribute to the establishment of focal adhesions and actin
stress fibers. Adhesion also affects additional actin-based
structures such as microspikes, membranous ruffles, and
morphological changes involved in cell spreading and motility (Craig and Johnson, 1996
; Zigmond, 1996
).
). Numerous proteins present at the cytoplasmic face of focal adhesions are considered to be structural
components of focal adhesions, including cytoskeletal proteins such as vinculin and talin (Jockusch and Rudiger,
1996
). In addition, numerous "signaling" proteins with enzymatic activity (e.g., kinases and GTPases) are also components of focal adhesions. By clustering these structural
and signaling components together, integrins transduce
the signals required to mediate certain aspects of cell physiology and morphology (Clark and Brugge, 1995
; Schwartz
et al., 1995
; Yamada and Geiger, 1997
). These signals may
include tyrosine phosphorylation of proteins such as paxillin and p130CAS (Burridge et al., 1992
; Petch et al., 1995
),
activation of protein tyrosine kinases such as the focal adhesion kinase (FAK) and Src (Schaller and Parsons, 1994
; Kaplan et al., 1995
), and activation of serine/threonine kinases such as the extracellular signal-regulated kinases
(Erks) or Akt (Chen et al., 1994
; Schlaepfer et al., 1994
;
King et al., 1997
).
),
similar to the response of cells to plating on an ECM-coated surface. Rho is also essential for the formation of
focal complexes (Hotchin and Hall, 1995
). The Rho-family
member Rac controls growth factor-stimulated membrane ruffling and formation of lamellipodia (Ridley et al.,
1992
). Finally, Cdc42 activation triggers the extension of
filopodia (Kozma et al., 1995
; Nobes and Hall, 1995
). Studies such as these have defined how soluble extracellular
factors induce the assembly of focal adhesions and stress
fibers in serum-starved adherent Swiss 3T3 fibroblasts
through activation of the Rho family of GTPases (Ridley,
1996
; Tapon and Hall, 1997
).
). More recently it was shown that
serum-starved Swiss 3T3 cells can form Rho-dependent
focal adhesions in the absence of added soluble factors if
they are given sufficient time (Barry et al., 1997
). Therefore, Rho activity is essential for focal adhesion formation
when cells are plated on a matrix, suggesting that Rho is
involved in signaling from integrin receptors. While these
studies have begun to define the mechanisms used by cells to generate morphological structures, a role for other Rho
family members has yet to be established. Furthermore,
the integrin-mediated signaling pathways regulated by the
Rho family have yet to be defined in detail. Therefore, our
goal in this study was to define the morphological changes
and integrin-mediated signaling pathways that are regulated by the Rho family GTPases, Cdc42, Rac, and Rho, in
response to plating cells on the ECM protein fibronectin, an insoluble stimulus, and in so doing, correlate changes in
morphology with changes in signaling.
Materials and Methods
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References
1 subunit antibody (130) was previously
characterized (Marcantonio and Hynes, 1988
).
a,b).
To enhance expression of N17Rac1 or N19RhoA, tetracycline was withdrawn 2 d before the cells were used. Rat1 lines expressing N17Cdc42
were derived as described (Qiu et al., 1997
). All cells were maintained at
37°C and 5% CO2 in DME, supplemented with 10% FBS, 2 mM
glutamine, penicillin at 400 units/ml, streptomycin at 150 µg/ml, and G418
at 400 µg/ml; puromycin at 2 µg/ml and tetracycline at 2 µg/ml were then
added to the N17Rac1- and N19RhoA-expressing Rat1 lines. Results observed in the N17Cdc42- and N17Rac1-expressing cell lines were confirmed in two clones, and results from the N19Rho-expressing line were
confirmed by treating Rat1 cells with the C3 transferase. C3 transferase (a
gift from M. Schwartz, Scripps Research Institute, La Jolla, CA) was introduced into Rat1 cells with Lipofectamine. C3 transferase (25 µg) and
Lipofectamine reagent (25 µg) were combined in 1 ml DME and incubated for 10 min at room temperature. An additional 4 ml of DME was
then added, and the solution was added to serum-starved cells for 4 h at
37°C. The cells were washed in PBS, trypsinized, and then prepared as described below. C3 transferase treatment caused the cells to retract lamellipodia and roundup, but the cells were viable and able to reattach and
spread (see Cell Preparation for Adhesion below).
). In brief, 1-11 µg of plasmid
DNA was coprecipitated with calcium phosphate and introduced to a 10-cm
dish of cells for 8 h. The cells were then rinsed and maintained in media
for an additional 48 h, followed by serum starvation as described below.
).
1 subunit (rabbit antiserum 130) and a fluorescein-conjugated secondary antibody.
). Briefly, proteins separated by SDS-polyacrylamide gels were transferred electrophoretically to nitrocellulose, which was blocked for 60 min
in 5% nonfat milk. The nitrocellulose was incubated for 120 min in primary antibody, washed for 30 min in Western Wash buffer (150 mM NaCl,
50 mM Tris, pH 7.6, 0.1% NP-40), incubated for 90 min in HRP-conjugated goat anti-mouse or goat anti-rabbit immunoglobulin, and then
washed for an additional 30 min in Western Wash buffer. The proteins were detected using a chemiluminescence detection kit (ECL; Amersham Corp., Arlington Heights, IL); three exposures of the immunoblot were
taken (15, 60, and 240 s) to insure linearity of the signal and the resulting
autoradiographic films quantitatively analyzed using an Is-1000 Digital
Imaging System (Alpha Innotech Corporation, San Leandro, CA).
-glycerophosphate, 40 mM para-nitrophenyl phosphate, 0.2 mM
Na3VO4, 1 mM PMSF, 1 µg/ml leupeptin, 1 µg/ml aprotinin). The lysates
were cleared by centrifugation at 16,000 g for 10 min at 4°C, and protein
concentration was determined as described above. Immunoprecipitation
was performed with 500 µg of lysate, 10 µg of 12CA5, and protein
A-Sepharose. The immunoprecipitates were then washed and subjected
to an in vitro kinase reaction (using myelin basic protein as substrate) as
described (King et al., 1997
). The specific activity of Erk2 was determined
by quantitating the 32P incorporated into myelin basic protein with a PhosphorImager and dividing it by the level of protein in the precipitate.
).
). In brief, serum-starved cells (800,000 cells/ml) suspended in 1 ml
of DME were plated on fibronectin- or polylysine-coated/BSA-blocked 35-mm wells for 15 or 60 min at 37°C. The cells were then washed twice in
PBS and fixed with 4% PFA in PBS for 10 min at room temperature. Next, the cells were permeabilized with 0.5% Triton X-100 in PBS for 10 min at room temperature, rinsed three times in PBS, and then F-actin was
stained with fluorescein-labeled phalloidin for 30 min at 37°C. After washing the cells three times with PBS, the labeled phalloidin was released from
the cells by adding 0.5 ml of methanol to each well. The cells were then
shaken for 2 h at room temperature in a dark humidified chamber and 0.3 ml of the solution was removed and measured using a spectrophotometer (excitation and emission wave lengths of 485 and 538 nm, respectively).
Results
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References
) or "focal complexes" (Nobes and Hall, 1995
). F-actin was also present at the cell perimeter
in membranous protrusions that appeared to be membrane ruffles (Fig. 1 a). At this time there were very few
cytoplasmic actin filaments that could be visualized. However, at 60 min there were many bundles of actin filaments
that spanned the central core of the cell (Fig. 1 c), and
these bundles appeared to be anchored at the cell periphery in vinculin-containing focal adhesions (Fig. 1 d).
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Fig. 1.
Assembly of focal adhesions and stress fibers during
adhesion and spreading of Rat1
fibroblasts on fibronectin. Serum-starved Rat1 fibroblasts (a-d) or
cells expressing dominant-inhibitory Cdc42 (e-h), Rac1 (I-L),
or RhoA (m-q) were trypsinized,
washed, and then replated on fibronectin-coated coverslips for
15 (a, b, e, f, i, j, m, and n) or 60 (c, d, g, h, k, l, p, and q) min and
stained for vinculin (b, d, f, h, j, l,
n, and q) and for F-actin (a, c, e,
g, i, k, m, and p). Bar, 10 µm.
These structures that formed when cells adhered and
spread on an insoluble ECM protein such as fibronectin
closely resembled the structures that form when adherent
Swiss 3T3 cells are stimulated with PDGF or serum (Ridley et al., 1992; Ridley and Hall, 1992
). To test whether
Rat1 cells were also susceptible to activation by these soluble
factors, serum-starved adherent Rat1 cells were treated
with buffer, PDGF, or FBS for 10 min, and then stained for F-actin. As shown in Fig. 2 a, some F-actin-containing
structures remained in serum-starved Rat1 cells, but serum treatment of these cells resulted in a dramatic increase in actin bundles (Fig. 2 c) and PDGF treatment induced the localization of F-actin into membrane ruffles
(Fig. 2 b). Therefore, both soluble and insoluble factors
are capable of stimulating the formation of cytoskeletal structures such as membrane ruffles, focal adhesions, and
actin bundles in Rat1 cells.
|
Cell Spreading Is Cdc42, Rac, and Rho Dependent
Whereas members of the Rho family of small GTPases are
known to regulate the assembly of cytoskeletal structures
stimulated by soluble factors, relatively little is known
about the role of the Rho family in integrin-mediated
changes in cell morphology. To examine this, we used
Rat1 cells that inducibly express dominant-inhibitory mutants of the Rho family members Cdc42, Rac1, and RhoA
(Fig. 3). The Rat1 cell lines selected for this study expressed the highest levels of N17Cdc42, N17Rac, and
N19Rho; they have been previously characterized (Qiu et al.,
1995a,b; Qiu et al., 1997
). The levels of expression of the
dominant-inhibitory mutants were sufficient to (1) inhibit
PDGF-induced membrane ruffling in N17Rac-expressing cells (Fig. 2 d), and (2) inhibit stress fiber formation induced by serum in N19Rho-expressing cells (Fig. 2 e).
|
We examined if the Rho-family members Cdc42, Rac, and/or Rho control a cell's ability to adhere to and/or spread on a matrix. Rat1 cells expressing dominant-inhibitory mutants of Cdc42, Rac, or Rho were plated onto fibronectin for 20 min, fixed, stained, and then the number of spread and nonspread cells counted. No significant differences between Rat1 cells and dominant-inhibitory Cdc42-, Rac-, or Rho-expressing cells were detected in the numbers of cells that adhered to fibronectin (data not shown). However, a significant reduction in cell spreading was observed in cells expressing dominant-inhibitory Cdc42, Rac, or Rho (Fig. 4).
|
A Role for Cdc42, Rac, and Rho in the Assembly of Focal Adhesions and Actin Fibers
To determine the roles of Rho family members in integrin-mediated cell morphology, Rat1 cells expressing dominant-inhibitory mutants of Cdc42, Rac, and Rho were plated onto fibronectin for 15 or 60 min and stained for F-actin. At the earliest time point examined (15 min), F-actin-containing ruffles were reduced in the N17Cdc42- and N17Rac-expressing cells (Fig. 1, e and i) when compared with the Rat1 control cells (Fig. 1 a). In contrast, membrane ruffles were prominent in N19Rho-expressing cells (Fig. 1 m), suggesting that both Cdc42 and Rac (but not Rho) are required for the adhesion-mediated organization of F-actin in membrane ruffles. Subsequent to membrane ruffling, F-actin was organized into bundles. The intensity of phalloidin-stained F-actin in stress fibers appeared to be reduced in the N17Cdc42- (Fig. 1 g) and N17Rac-expressing (Fig. 1 k) cells, and N19Rho expression completely blocked the assembly of these structures (Fig. 1 p). To quantitate the reduction in F-actin observed in Rat1 cells expressing dominant-inhibitory mutants of Cdc42, Rac, or Rho, the cells were plated on fibronectin for 15 or 60 min and the amount of labeled phalloidin bound to F-actin quantitated as described in Materials and Methods. In the cells expressing dominant-inhibitory mutants of Cdc42, Rac, and Rho, F-actin levels were reduced to <50% of the level in control Rat1 cells (Fig. 5), suggesting that Cdc42, Rac, and Rho all control the rise in F-actin associated with adherence to fibronectin.
|
In parallel with our observations on actin organization, we stained the fibronectin-adherent cells for vinculin-containing focal adhesions. In the N17Cdc42- and N17Rac- expressing cells at the 60-min time point (Fig. 1, h and l, respectively), the size and/or number of vinculin-containing focal adhesions appeared reduced relative to the Rat1 control cells (Fig. 1 d). In contrast, small focal complexes were observed in the N19Rho-expressing cells at 15 min (Fig. 1 n), but the staining was more dramatically reduced by 60 min so that only a few faint focal adhesions were observed (Fig. 1 q). These results suggest that Rho is essential for adhesion-dependent focal adhesion assembly, but that Cdc42 and Rac also play a role in the assembly of these structures.
To insure that the effects on cell morphology observed in cells expressing N17Cdc42, N17Rac, or N19Rho were not due to an adaptive response of the cells to the low levels of expression of the dominant-inhibitory mutants in uninduced cells, we performed transient transfections of N17Cdc42 or N17Rac1, or treated Rat1 cells with the Rho-specific inhibitor C3 transferase and monitored the adhesion-mediated organization of focal contacts and actin stress fibers. Cells expressing high levels of N17Cdc42 retained a rounded morphology 60 min after being plated onto fibronectin, and these cells had few (if any) detectable actin fibers (Fig. 6 a) or focal contacts (Fig. 6 c). Under the conditions of this experimental system, N17Cdc42 expression had a more pronounced effect on cell morphology (when compared with the stably expressing N17Cdc42 cell lines), perhaps resulting from the higher level of N17Cdc42 expression obtained (Fig. 6, b and d; and data not shown). Transient expression of N17Rac also inhibited the organization of actin fibers (Fig. 6 e) and focal contacts (Fig. 6 g), similar to what we observed in cells using the tetracycline-repressible system to express N17Rac. C3 transferase inhibition of Rho blocked adhesion-induced stress fiber formation (Fig. 6 i) and focal adhesion organization (Fig. 6 j) in a manner similar to that observed in the N19Rho-expressing Rat1 cells (Fig. 1, m and n), with only peripheral point contacts and actin fibers detected. These results, obtained in transiently transfected or C3-treated Rat1 cells, serve to support the hypothesis stated above that Cdc42, Rac, and Rho control the assembly of focal adhesions and actin filaments; they suggest that Cdc42 and Rac control the generation of peripheral adhesion/cytoskeletal structures that are generated during cell spreading (an event that may indirectly control stress fiber formation), whereas Rho more directly controls the generation of focal adhesions and actin stress fibers.
|
Adhesion to Fibronectin Induces Integrin-mediated Signals Regulated by Rho and Cdc42
Not only do focal adhesions function as sites where structural components of a cell are assembled to regulate cell
morphology, they also serve as a framework for the organization of signaling complexes that control integrin-mediated changes in cell behavior. Since the Rho family of
small GTPases regulates several aspects of cell morphology as described above, we set out to identify integrin-
mediated signaling events that are Cdc42, Rac, and/or Rho
dependent. Since protein phosphorylation is one of the
earliest events detected in response to integrin engagement (Guan et al., 1991; Kornberg et al., 1991
), we chose
to examine the role of Rho family GTPases in integrin-mediated tyrosine phosphorylation by observing: (a) the
activation of several kinases (FAK, Src, Erk, and AKT)
that are known to be activated by integrin stimulation; and
(b) the tyrosine phosphorylation of some of the potential substrates of these kinases (FAK, Erk, and paxillin).
FAK, a tyrosine kinase that is activated and tyrosine
phosphorylated in response to integrin-matrix interactions
(Hanks et al., 1992; Kornberg et al., 1992
; Lipfert et al.,
1992
), is also phosphorylated in response to agents that activate Rho (Zachary et al., 1992
; Kumagai et al., 1993
).
This makes FAK a likely candidate to be regulated by the
Rho family of GTPases in response to adhesion. FAK tyrosine phosphorylation was monitored by immunoblotting
FAK immunoprecipitates with a phosphotyrosine antibody (Fig. 7). FAK tyrosine phosphorylation in response
to plating of Rat1 cells on fibronectin was unaffected
in N17Cdc42- or N17Rac-expressing cells (<10%), but
N19Rho expression inhibited the tyrosine phosphorylation of FAK by 50% (n = 5) at the 90-min time point (Fig.
7 A). Furthermore, when Rat1 cells were treated with C3
transferase to inhibit Rho activity, FAK tyrosine phosphorylation was inhibited by 40-60% (n = 3) (Fig. 7 C). Interestingly, we did not observe any inhibition of FAK tyrosine phosphorylation in the N19Rho-expressing cells at
the 10-min time point (<5%, n = 5), suggesting that Rho
may not be required for the earliest stages of FAK activation.
|
Paxillin is another focal adhesion protein that is tyrosine
phosphorylated in response to integrin-matrix interactions (Burridge et al., 1992). In fact, it is a likely substrate
for FAK. Paxillin tyrosine phosphorylation in response to
adhesion of Rat1 cells to fibronectin was monitored by
anti-phosphotyrosine immunoblotting of paxillin immunoprecipitates. Paxillin tyrosine phosphorylation was reduced by 70% (n = 3) in the N19Rho-expressing cells at
the 90-min time point (Fig. 8 A) and 50% (n = 2) in C3
transferase-treated cells (Fig. 8 C), suggesting that the tyrosine phosphorylation of both FAK and paxillin is at least
in part dependent on Rho.
|
The mitogen-activated protein (MAP) kinase Erk2 can
be activated and phosphorylated downstream of FAK in
an integrin-dependent manner (Chen et al., 1994;
Schlaepfer et al., 1994
). Since Erk2 activation is associated
with a decrease in the electrophoretic mobility of the protein (or upward shift), we chose to examine the role of
Rho family GTPases on Erk2 activity in response to plating of Rat1 cells on fibronectin by observing the mobility shift. Erk2 activation in Rat1 cells was rapid and transient, peaking at ~15 min, and then returning to near basal levels by 90 min (Fig. 9). All three dominant-inhibitory mutants (Fig. 9 A) and C3 transferase treatment (Fig. 9 C)
diminished the activation of Erk2 to varying degrees
(N17Cdc42 > C3 > N19Rho > N17Rac), with N17Cdc42
completely inhibiting integrin-mediated Erk2 activation and N17Rac having very little effect. Furthermore, adhesion-induced activation of Erk2 kinase activity, which was
enhanced fourfold upon adhesion to fibronectin, was also
inhibited when N17Cdc42 was transiently coexpressed
(Fig. 9 D). Interestingly, N17Cdc42 expression did not inhibit Erk2 activation in PDGF-stimulated cells (Fig. 9 B),
suggesting that Cdc42 may strictly be involved in integrin-mediated Erk2 activation.
|
Src is another protein tyrosine kinase activated upon integrin engagement that may play a role in signaling from
the focal adhesion complex (Kaplan et al., 1995). To examine the role of Rho-family GTPases on Src activity in response to plating cells on fibronectin we immunoblotted
detergent lysates with an antibody that specifically recognizes Src when it is phosphorylated at its autophosphorylation site (tyrosine 416) (Gao et al., 1997
). Src was rapidly
autophosphorylated in response to plating of Rat1 cells on
fibronectin (Fig. 10). However, no significant difference (<15%) was observed in Src autophosphorylation in Rat1
cells expressing dominant-inhibitory Rho family mutants,
suggesting that integrin-mediated activation of Src is independent of Cdc42, Rac, and Rho.
|
Akt, an effector for phosphatidylinositol (PI) 3-kinase,
is another kinase that can be activated upon attachment to
fibronectin (King et al., 1997). We examined the potential
role of Rho family GTPases in integrin-mediated Akt activation by performing immunoprecipitation/kinase assays
using histone H2B as an exogenous substrate. Akt activity, which was activated threefold when Rat1 cells were plated
on fibronectin, was unaffected by N17Rac or N19Rho expression but inhibited by 55% (n = 3) in N17Cdc42-
expressing Rat1 cells (Fig. 11 A). The adhesion-induced
Cdc42-dependent activation of Akt was further confirmed
by transient co-transfection of N17Cdc42 and HA-tagged Akt in Rat1 cells. The twofold activation of Akt, observed
after plating these cells on fibronectin for 15 and 60 min,
was blocked by N17Cdc42 (Fig. 11 B). These data suggest
that Cdc42 is necessary for integrin-dependent activation
of Akt (and, by inference, PI 3-kinase).
|
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Discussion |
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---|
Numerous studies have examined how soluble extracellular factors induce the assembly of focal adhesions and
stress fibers in serum-starved adherent Swiss 3T3 fibroblasts through activation of the Rho family of GTPases
(Tapon and Hall, 1997; Van Aelst and D'Souza-Schorey,
1997
). In contrast, relatively little is known in detail about
how these same actin-based structures are established de
novo when cells adhere to the ECM via integrins. Therefore, the purpose of this study was to define the roles of the Rho family GTPases Cdc42, Rac, and Rho in (a) morphological changes that accompany cell adhesion and
spreading on the ECM, and (b) regulating integrin-mediated signaling pathways.
Rho Family Members and Cell Morphology
We observed that inhibition of Cdc42, Rac, or Rho did not
significantly affect Rat1 cell adhesion to fibronectin. Rho
inhibition can block agonist-induced lymphocyte adhesion, suggesting that Rho family members can control integrin-mediated cell adhesion in some (but not all) cell types
(Laudanna et al., 1996). In contrast to cell adhesion, we
found that the process of cell spreading is dependent on
Cdc42, Rac, and Rho. This observation is in agreement with Barry et al. (1997)
who found that Swiss 3T3 cells
treated with the C3 transferase were poorly spread, suggesting that Rho regulates spreading in these cells. However, Rho can function as a negative regulator of cell
spreading in phorbol ester-treated monocytes (Aepfelbacher et al., 1996
) or in thrombin-stimulated neuronal
cells (Jalink et al., 1994
), suggesting that Rho may positively or negatively regulate the process of cell spreading in different cell types.
During cell spreading on fibronectin, a series of morphological changes transpires: first, Rat1 cells assemble actin-containing projections, membranous ruffles, and punctate vinculin-containing point contacts or focal complexes at the cell periphery; second, the focal complexes are replaced by full-blown focal adhesions from which bundles of F-actin radiate; and finally, there is an enhancement of F-actin-rich structures as the cells continue to spread on fibronectin. Our results suggest that most of these structures are regulated by Rho family GTPases but that different aspects require different GTPases (see Fig. 12 A). As it happens, the differing effects of the different dominant- inhibitory mutants provide evidence for the selectivity of these reagents.
|
N19Rho expression inhibits the matrix-induced assembly of focal adhesions and actin stress fibers, as has been
observed previously (Hotchin and Hall, 1995; Barry et al.,
1997
). However, we found that, early in the process of cell
spreading, the small, vinculin-containing focal complexes
that form do so in a Rho-independent manner. However, Rho appears to be required for the clustering of these
complexes into larger focal adhesions. This hypothesis is
supported by the observation that integrin cross-linking
(which may occur when cells adhere to fibronectin) can induce Rho clustering at these sites (Burbelo et al., 1995
) as
a precursor to the Rho-dependent generation of cell contraction that stimulates the aggregation of integrins into
larger focal adhesions (Chrzanowska-Wodnicka and Burridge, 1996
). Taken together, these results suggest that when suspended cells are plated on ECM substrates, they
first assemble small focal complexes that subsequently
cluster into larger focal adhesions in a Rho-dependent
manner; this latter stage may be analogous to the assembly
of focal adhesions in serum-starved adherent cells stimulated with Rho-activating agents such as lysophosphatidic acid (LPA) (Ridley and Hall, 1992
). Expression of moderate levels of N17Cdc42 or N17Rac in Rat1 cells had a
modest effect on the generation of focal adhesions, causing an apparent delay in the assembly of the larger focal
adhesions. However, transient overexpression of N17Cdc42
had a more dramatic effect on the generation of focal adhesions than that observed in the cells expressing
N17Cdc42 stably. Taken together, these results suggest
that Rho is the primary small GTPase regulating focal adhesions, but Cdc42 may also play a prominent role.
In contrast to focal adhesion assembly, we observed that
the generation of microfilament bundles when Rat1 cells
adhere to fibronectin is regulated by all three small GTPases. At first glance, this observation would appear to suggest that focal adhesions can be generated without prior
assembly of actin stress fibers, a conclusion supported by a
previous study in which Rho-induced focal adhesion assembly occurred in the presence of inhibitors of actin filament assembly (Nobes and Hall, 1995). However, it should
be noted that whereas the actin bundles observed in cells expressing N17Cdc42, N17Rac, and N19Rho were much
less intense than those observed in Rat1 control cells, every vinculin-containing focal adhesion appears to be associated with a bundle of actin filaments. This observation is
consistent with the idea that focal adhesions form as a result of the contractility of stress fibers (Chrzanowska-Wodnicka and Burridge, 1996
).
The precise mechanism whereby Rho family GTPases
regulate the assembly of the F-actin component of actin
stress fibers has been the subject of numerous studies. It
has been suggested that Rho regulates stress fiber formation in mitogen-stimulated Swiss 3T3 cells by inducing the
bundling of existing F-actin into larger actin stress fibers
and not by inducing actin polymerization (Machesky and
Hall, 1997); we have found, using a different assay, that Rho is required for an adhesion-induced increase in F-actin.
The apparent discrepancy is likely to be the result of differences in the mechanisms by which insoluble (matrix)
and soluble factors stimulate actin polymerization, as has
been previously noted in other cell types (Southwick et al.,
1989
). It is also possible that in our assay we are measuring
a Rho-dependent stabilization of F-actin rather than de
novo actin polymerization. We have also observed that
Rac is required for adhesion-induced actin polymerization; this finding is in keeping with observations that Rac
(but not Rho) is essential for the uncapping of actin filaments (Hartwig et al., 1995
), and subsequent actin monomer incorporation into stress fibers and lamellipodia (Machesky and Hall, 1997
). Furthermore, it was recently
observed that Cdc42 (but not Rho or Rac) regulates actin
polymerization in a cell-free system (Zigmond et al., 1997
; Ma et al., 1998
), and we observed that N17Cdc42 expression inhibited the adhesion-induced increase in F-actin. Finally, genetic evidence suggests that both Rac and Cdc42
control actin-dependent processes; Rac controls actin assembly at adherens junctions and Cdc42 controls actin-
dependent epithelial cell polarization in Drosophila
(Eaton et al., 1995
). Taken together, these results suggest
that Rho, Rac, and Cdc42 are all capable of regulating
F-actin content within a cell and that all three do so upon
cell adherence. The possible mechanisms whereby Rho
family GTPases regulate integrin-dependent actin organization will be discussed below.
Rho Family Members in Integrin-mediated Signaling
Numerous signal transduction molecules associate with integrin complexes in adherent cells, placing them in an
ideal location to respond to and regulate adhesion-dependent changes in cell morphology (Yamada and Miyamoto,
1995). One of the earliest responses to integrin-dependent
adhesion is tyrosine phosphorylation (Guan et al., 1991
;
Kornberg et al., 1991
), a response that is also elicited by
LPA in a Rho-dependent manner (Barry and Critchley, 1994
; Ridley and Hall, 1994
; Flinn and Ridley, 1996
). These
results suggest that Rho could function downstream of integrins in directing adhesion-dependent tyrosine phosphorylation. Tyrosine kinase(s) may also function upstream of
Rho since the tyrosine kinase inhibitor tyrphostin A25,
which blocks the assembly of LPA-induced focal adhesions, can be overcome by microinjection of activated Rho (Nobes et al., 1995
). Therefore, protein tyrosine kinases
appear to play a role both upstream and downstream of
Rho in integrin-mediated tyrosine phosphorylation.
There are several candidate kinases that may be responsible for integrin-dependent tyrosine phosphorylation,
most notably FAK and Src. FAK is tyrosine phosphorylated and activated in response to integrin stimulation or
upon LPA stimulation, and LPA-mediated FAK phosphorylation is Rho dependent (Hanks et al., 1992; Kornberg et al., 1992
; Lipfert et al., 1992
; Kumagai et al., 1993
; Seckl et al., 1995
). We found that Rho, but not Rac or Cdc42, is
required for the complete phosphorylation of FAK induced by matrix adhesion; this result is in agreement with
Barry et al. (1997)
, suggesting that FAK activation is in
part Rho dependent. However, we observed that at the
earliest times examined FAK phosphorylation was not inhibited by N19Rho expression, suggesting that there is a
Rho-independent component to integrin-dependent FAK
phosphorylation. This observation is supported by data
from Miyamoto et al. (1995)
showing that aggregation of
integrins can cause FAK to be phosphorylated without the
accumulation of Rho-dependent F-actin. Furthermore,
since there are multiple sites on which FAK is phosphorylated, it is possible that Rho may regulate some but not all
of these sites. One of these sites is tyrosine 397 that, when
phosphorylated, creates a docking site for the Src homology 2 (SH2) domain when cells adhere to fibronectin
(Schlaepfer et al., 1994
). Since the Src SH2 domain normally interacts with the phosphorylated tyrosine 527 at
Src's COOH terminus, an interaction that maintains Src in
a low activity state (Parson and Parsons, 1997
), association of Src with FAK may occur concomitantly with Src activation. Src is activated in an integrin-dependent manner after
cell adhesion (Kaplan et al., 1995
). We found that plating
of Rat1 cells on fibronectin induced the phosphorylation
of Src at tyrosine 416, the autophosphorylation site that is
phosphorylated in activated forms of Src. The phosphorylation of Src was not dependent on Cdc42, Rac, or Rho,
suggesting that Src is unlikely to be one of the kinases
downstream of Rho family GTPases.
After integrin-dependent assembly of the FAK-Src
complex, additional signaling elements may then be recruited to link adhesion to activation of the Ras/MAP kinase pathway (Chen et al., 1994; Schlaepfer et al., 1994
;
Clark and Hynes, 1996
). Integrin-dependent activation of
the MAP kinase Erk2 is partially inhibited by N19Rho expression or C3 transferase treatment, suggesting that Rho
plays a role in the activation of this kinase (Renshaw et al.,
1996
). We found that in addition to Rho playing a role in
Erk2 activity, Cdc42 also regulates Erk2 activation since
N17Cdc42 expression almost completely inhibited Erk2
phosphorylation and kinase activity. All three Rho family
GTPases may control Erk2 activity through their effects
on the cytoskeleton, since treatment of cells with cytochalasin D inhibits adhesion-induced (but not growth factor- induced) Erk2 activity (Chen et al., 1994
). Interestingly,
N17Cdc42 expression did not affect PDGF-induced Erk2
activation, suggesting that growth factor activation of Erk2
is Cdc42 independent. This result is in contrast to our earlier observation that Ras controls both integrin-dependent
and growth factor-induced activation of Erk2 (Clark and
Hynes, 1996
), which suggests that Ras and Cdc42 control
integrin-dependent Erk2 activation by distinct mechanisms. Cdc42 regulation of adhesion-dependent Erk2 activation may play a role in the control of anchorage-independent growth by Cdc42 (Qiu et al., 1997
).
The cytoskeletal protein paxillin is another potential effector for the FAK-Src complex. Paxillin colocalizes with
FAK to focal adhesions, can physically associate with
FAK, and is tyrosine phosphorylated in response to adhesion to fibronectin (Burridge et al., 1992; Clark and
Brugge, 1995
). In addition, overexpression of a FAK mutant that is incapable of binding to Src fails to induce paxillin phosphorylation, and expression of the COOH-terminal domain of FAK inhibits both FAK activation and
paxillin tyrosine phosphorylation (Schaller and Parsons,
1995
; Richardson and Parsons, 1996
). Paxillin could be a
downstream mediator of signals initiated by FAK, since
paxillin tyrosine phosphorylation, like that of FAK, is at
least partially Rho-dependent (Rankin et al., 1994
; Barry
et al., 1997
). It has been suggested that the tyrosine phosphorylation of paxillin may recruit SH2-containing proteins essential for focal adhesion formation to sites of adhesion (Turner, 1993
). However, while FAK inhibition
blocks adhesion-associated tyrosine phosphorylation, it
does not inhibit focal adhesion assembly (Gilmore and
Romer, 1996
). Our finding that paxillin tyrosine phosphorylation does not proceed in N19Rho-expressing cells that
fail to assemble focal adhesions is consistent with the hypothesis that Rho-dependent focal adhesion formation
regulates paxillin tyrosine phosphorylation (Burridge and
Chrzanowska-Wodnicka, 1996
).
The serine-threonine kinase Akt (also known as PKB),
a close relative of protein kinase C, has been shown to be a
target of PI 3-kinase (Datta et al., 1996; Franke et al.,
1997
). Integrin-dependent activation of Akt also requires
PI 3-kinase (King et al., 1997
). Our finding that integrin-dependent activation of Akt is suppressed when
N17Cdc42-expressing Rat1 cells are plated on fibronectin therefore suggests that Cdc42 lies upstream of PI 3-kinase
and Akt in an integrin-mediated signaling pathway. This
result is consistent with several observations: first, that
Cdc42 binds the regulatory subunit of PI 3-kinase, stimulating PI 3-kinase activity (Zheng et al., 1994
); and second,
that Cdc42 induces integrin-mediated cell motility through
a PI 3-kinase-dependent pathway (Keely et al., 1997
). In
addition, integrin-mediated PI 3-kinase activity is sufficient to stimulate Rac-dependent ruffling (Reif et al.,
1996
; Rodriguez-Viciana et al., 1997
). We therefore propose an integrin-mediated signaling pathway that includes
Cdc42-dependent activation of PI 3-kinase, PI 3-kinase-
dependent activation of Rac, and Rac-dependent generation of membrane ruffles (see Fig. 12 B). This pathway
may also regulate integrin-dependent Erk2 phosphorylation, since both Cdc42 (Fig. 9) and PI 3-kinase (King et al.,
1997
) contribute to Erk2 phosphorylation that occurs
when cells adhere to fibronectin.
The Role of Rho Family GTPases in Integrin-mediated Cytoskeletal Organization
We have provided evidence that, like soluble factors, the
ECM controls cytoskeletal organization through several
distinct signal transduction pathways regulated by different members of the Rho family of GTPases (see Fig. 12
B). When cells initially adhere to the ECM, punctate focal
complexes form at the cell periphery. These clusters of adhesion molecules are capable of initiating some signaling
involving FAK and Src independent of Rho family GTPases (Fig. 12 B, top). However, subsequent FAK activation
occurs somewhat more slowly in a Rho-dependent manner. This Rho-dependent pathway also controls paxillin
phosphorylation and the assembly of large focal adhesions
from which actin stress fibers radiate. Initial adhesion also
stimulates peripheral F-actin staining in membrane ruffles.
This actin organization is independent of Rho but is dependent on both Cdc42 and Rac (Fig. 12 B, bottom). Since Cdc42 controls the integrin-dependent activation of Akt
(whose activation is PI 3-kinase-dependent; King et al.,
1997), and since PI 3-kinase can stimulate Rac-dependent
membrane ruffling, Cdc42, PI 3-kinase, and Rac could lie
on the same pathway that regulates adhesion-induced
membrane ruffling. Finally, significant cross-talk between
these pathways is likely: the Cdc42- and Rho-dependent pathways may regulate cell proliferation via the Ras/Raf
pathway (Schlaepfer et al., 1994
; King et al., 1997
).
The pathways defined in the model we present for adhesion-induced signaling (Fig. 12 B) are the result of combining the results obtained in this study with several recent
observations (Clark and Hynes, 1996; King et al., 1997
;
Qiu et al., 1997
; Rodriguez-Viciana et al., 1997
). Our
model, in which Ras, Rac, Rho, and Cdc42 play roles in
parallel, convergent pathways, can be contrasted to the "cascade" model for regulation of Rho family GTPases
that has solidly established itself as a paradigm in the field
(Chant and Stowers, 1995
). This model is based, in large
part, on microinjection studies in serum-starved Swiss 3T3
fibroblasts that have indicated that Rho family GTPases
can function in a cascade-like fashion to organize the actin
cytoskeleton with Cdc42 activating Rac, which activates
Rho (Nobes and Hall, 1995
). However, other studies in
Swiss 3T3 fibroblasts have shown that constitutively active Cdc42 causes stress fiber disassembly (Kozma et al., 1995
).
In addition, in neuronal cells an antagonistic relationship
has been noted between Rho on the one hand, and Cdc42
and Rac on the other (Kozma et al., 1997
). Finally, our results detailing the regulation of FAK and paxillin tyrosine
phosphorylation by Rho, but not by Rac or Cdc42, suggest
that these GTPases are not controlled in a simple linear
cascade (from Cdc42 to Rac to Rho) during cell adhesion
and spreading on fibronectin. Instead, Cdc42- and Rac- dependent pathways converge with Rho-dependent pathways to control cell morphology (see Fig. 12 B). Taken together, these results suggest that alternatives to the
cascade model may describe the signaling pathways regulated by Rho family GTPases in other systems (e.g., adhesion-based signaling).
These independent pathways are envisaged as converging, through their effects on focal adhesion assembly and/
or actin microfilament organization, to give order to the
actin-based cytoskeleton. While this skeleton plays a vital
role in cell morphology and movement, it can also affect
MAP kinase signaling pathways that regulate gene expression and cell proliferation (Schwartz et al., 1995). How
might Cdc42, Rac, and Rho regulate the integrin-mediated
organization of this actin-based cytoskeleton? Cdc42 and
Rac may do so through the serine/threonine kinase Pak1
(p21-activated kinase), since activated Pak1 is capable of
inducing filopodia and ruffles (Sells et al., 1997
). However,
Cdc42- and Rac-effector mutants that do not interact with
Pak1 still induce cytoskeletal changes (Joneson et al.,
1996
; Lamarche et al., 1996
), suggesting that Cdc42 and Rac may use several pathways to regulate actin organization. Cdc42 may regulate the actin cytoskeleton through
its association with the Wiskott-Aldrich Syndrome protein, a protein whose absence in affected individuals
causes cytoskeletal abnormalities of T cells and platelets
(Symons et al., 1996
). Rac also stimulates the uncapping of
actin filaments through a polyphosphoinositide (PPI)-
dependent mechanism (Hartwig et al., 1995
), suggesting that Rac may control cytoskeletal organization through
regulation of PPI synthesis. Rho may also control the synthesis of cellular PPIs by regulating the phosphatidylinositol 4-phosphate (PIP) 5-kinase (Chong et al., 1994
). These
results suggest that Rac and/or Rho may regulate actin organization by controlling the cellular levels of PPIs in a
Rac- or Rho-dependent manner. Further evidence that
PIP2 may play a role in cytoskeletal organization comes
from studies on PIP 5-kinase suggesting that PIP2 is capable of inducing actin polymerization in vivo (Shibasaki et
al., 1997
). PIP2 may then regulate the organization of the
cytoskeleton through regulation of vinculin binding to
talin and actin (Gilmore and Burridge, 1996
; Weekes et
al., 1996
). However, stimulation of actin polymerization in
a cell-free system does not correlate with PIP2 synthesis
(Zigmond et al., 1997
), indicating that additional work
must be done in defining the role of PIs in regulating the actin cytoskeleton. Finally, several RhoA-binding kinases
(ROK
, Rho-kinase) are capable of promoting the formation of focal adhesions and actin stress fibers when introduced into cells (Leung et al., 1996
; Amano et al., 1997
;
Ishizaki et al., 1997
). These Rho-binding kinases can phosphorylate the myosin-binding subunit of myosin light
chain (MLC) phosphatase (thereby inactivating the phosphatase, resulting in MLC phosphorylation) and MLC itself (Amano et al., 1996
; Kimura et al., 1996
). Phosphorylated MLC induces a conformational change in myosin,
increasing its binding to actin filaments and triggering the
assembly of stress fibers (Citi and Kendrick-Jones, 1987
;
Burridge and Chrzanowska-Wodnicka, 1996
). Rho kinase
may also regulate the cytoskeleton by phosphorylating the
ezrin/radixin/moesin proteins (Matsui et al., 1998
; Shaw et
al., 1998
). These results suggest that Rho kinases may be
important effectors for Rho in controlling the organization
of the cytoskeleton.
In summary, we have shown that adhesive interactions stimulate the formation of punctate focal complexes at the cell periphery during cell spreading, and cell spreading is dependent on Cdc42, Rac, and Rho. Membrane ruffling occurs through a mechanism that is dependent on Cdc42 and Rac, whereas Rho controls the assembly of large focal adhesions from which actin stress fibers radiate. Rho also regulates the majority of FAK activation and the phosphorylation of paxillin, events that may modulate the organization of the cytoskeleton during the assembly of Rho-dependent focal adhesions, whereas Cdc42 regulates adhesion-associated Akt and Erk2 activation.
![]() |
Footnotes |
---|
Received for publication 21 November 1997 and in revised form 19 May 1998.
Address all correspondence to Richard O. Hynes, Massachusetts Institute of Technology, Center for Cancer Research, 77 Massachusetts Avenue, E17-227, Cambridge, MA 02139. Tel.: (617) 253-6422. Fax: (617) 253-8357. E-mail: rohynes{at}mit.eduWe are grateful to J.-L. Guan, A. Laudano, and P. Tsichlis for antibodies to FAK, Src, and Akt.
This work was supported by grants CA17007 (to R.O. Hynes) and CA47572 (to J.S. Brugge) from National Cancer Institute and by the Howard Hughes Medical Institute (R.O. Hynes). E.A. Clark is a Charles A. King Trust Research Fellow. R.O. Hynes is an investigator and E.A. Clark was an associate of the Howard Hughes Medical Institute.
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Abbreviations used in this paper |
---|
ECM, extracellular matrix; Erk, extracellular signal-regulated kinase; F-actin, filamentous actin; FAK, focal adhesion kinase; HA, hemagglutinin; LPA, lysophosphatidic acid; MAP, mitogen-activated protein; PI, phosphatidylinositol; SH2, Src homology 2.
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