ICAP-1, a Novel beta 1 Integrin Cytoplasmic Domain-associated Protein, Binds to a Conserved and Functionally Important NPXY Sequence Motif of beta 1 Integrin

David D. Chang, Carol Wong, Healy Smith, and Jenny Liu

Department of Medicine, and Department of Microbiology and Immunology, University of California, Los Angeles, California 90095

Abstract
Materials and Methods
Results
Discussion
Footnotes
Acknowledgements
Abbreviations used in this paper
References


Abstract

The cytoplasmic domains of integrins are essential for cell adhesion. We report identification of a novel protein, ICAP-1 (integrin cytoplasmic domain- associated protein-1), which binds to the beta 1 integrin cytoplasmic domain. The interaction between ICAP-1 and beta 1 integrins is highly specific, as demonstrated by the lack of interaction between ICAP-1 and the cytoplasmic domains of other beta  integrins, and requires a conserved and functionally important NPXY sequence motif found in the COOH-terminal region of the beta 1 integrin cytoplasmic domain. Mutational studies reveal that Asn and Tyr of the NPXY motif and a Val residue located NH2-terminal to this motif are critical for the ICAP-1 binding. Two isoforms of ICAP-1, a 200-amino acid protein (ICAP-1alpha ) and a shorter 150-amino acid protein (ICAP-1beta ), derived from alternatively spliced mRNA, are expressed in most cells. ICAP-1alpha is a phosphoprotein and the extent of its phosphorylation is regulated by the cell-matrix interaction. First, an enhancement of ICAP-1alpha phosphorylation is observed when cells were plated on fibronectin-coated but not on nonspecific poly-L-lysine-coated surface. Second, the expression of a constitutively activated RhoA protein that disrupts the cell-matrix interaction results in dephosphorylation of ICAP-1alpha . The regulation of ICAP-1alpha phosphorylation by the cell-matrix interaction suggests an important role of ICAP-1 during integrin-dependent cell adhesion.


INTEGRINS comprise a family of heterodimeric cell adhesion receptors responsible for attachment of cells to the extracellular matrix or to specific cell surface counterreceptors (Hynes, 1992). Each subunit consists of a large extracellular domain that participates in the ligand recognition, a transmembrane region, and a short cytoplasmic domain. In adherent cells, the ligand binding induces recruitment of integrins to the focal adhesion plaques or focal contacts, where actin cytoskeletons converge onto the site of cell-extracellular matrix contact (for review see Burridge and Chrzanowska-Wodnicka, 1996). Studies have shown that the integrin-dependent cell adhesion can be regulated either by direct affinity modulation of integrins (Bennett and Vilaire, 1979; Altieri and Edgington, 1988; Faull et al., 1993; Stewart et al., 1996) or by clustering of integrins, which requires cytoskeletal rearrangement (Hermanoswki-Vosatka et al., 1988; Haverstick et al., 1992; van Kooyk et al., 1994; Stewart et al., 1996). Either through recruitment of regulatory proteins such as adaptor protein Shc or focal adhesion kinase (FAK)1 to the focal contacts or by inducing reorganization of actin cytoskeleton, integrins function as transmembrane receptors for extracellular signals and participate in the activation of cytoplasmic signaling cascade (for review see Schwartz et al., 1995). The dependence of cell proliferation, prevention of apoptosis, and cell differentiation on the cell-matrix interaction mediated by integrins illustrates the importance of this adhesion-dependent cell signaling.

Although the cytoplasmic domains of integrins lack any known enzymatic activity or sequence motif involved in protein-protein interaction, studies have shown that the short cytoplasmic tails of alpha  or beta  subunits are important for the regulation of integrin affinity and cytoskeletal interaction (Sastry and Horwitz, 1993; Schwartz et al., 1995). The cytoplasmic domains of different beta subunits are similar in size and sequence. Mutational analysis of the cytoplasmic domain of integrin beta 1 has identified three regions that are important for the recruitment of integrins to the focal contacts (Marcantonio et al., 1990; Reszka et al., 1992). The first region, located in the membrane-proximal region, is rich in charged residues and predicted to form an alpha -helical structure. The second and third region consist of short sequences Asn-Pro-X-Tyr (NPXY). The NPXY motif was initially recognized as a sequence motif required for receptor-mediated endocytosis (Chen et al., 1990) and represents a unique structural motif capable of generating a reverse turn in solution (Bansal and Gierasch, 1991). The two tandem NPXY motifs of the integrins are situated in the membrane-distal region that is known to undergo alternative splicing (Languino and Ruoslahti, 1992; Zhidkova et al., 1995). Naturally occurring splicing variants of the beta 1 integrin lacking the NPXY motifs do not localize to the focal contacts (Balzac et al., 1993). The first NPXY motif (membrane-proximal), in addition to playing a role in integrin- cytoskeleton interaction (Reszka et al., 1992; Ylanne et al., 1995), is also involved in affinity regulation of integrins (O'Toole et al., 1995) and integrin-dependent endocytic processes (Van Nhieu et al., 1996). Mutational studies have shown that the second NPXY motif (membrane-distal), like the first NPXY motif, is important for the focal contact localization of beta 1 integrins (Reszka et al., 1992) and cell adhesion by the beta 2 integrins (Hibbs et al., 1991; Peter and O'Toole, 1995).

How the integrin beta  subunit cytoplasmic domain participates in the regulation of cell-matrix interaction has not been resolved. The initial molecular models for the adhesion-dependent recruitment of integrins to the focal contacts were based on the observation that talin (Horwitz et al., 1986) and alpha -actinin (Otey et al., 1990) bind to the beta 1 integrins. As both of these proteins can bind actin, either directly as in alpha -actinin or through interaction with vinculin as in talin, the proposed function of talin and alpha -actinin in linking integrins to the cytoskeletal structures remains an attractive model. Other proteins that have been shown to bind beta 1 integrins include FAK (Schaller et al., 1995), paxillin (Schaller et al., 1995), and a Ser/Thr kinase (ILK-1) (Hannigan et al., 1996). Of these proteins, FAK and ILK-1, because of their ability to affect cell adhesion and cell spreading, represent potential regulators of the integrin-matrix interaction.

A second unresolved issue is whether the adhesive function and cytoskeletal interaction of different integrins are regulated by a common mechanism or by similar but distinct processes. Despite the remarkable similarities in the amino acid sequences of different integrin beta  subunit cytoplasmic domains, each beta  subunit displays distinct differences in its ability to localize to the focal contacts (Wayner et al., 1991; LaFlamme et al., 1994), to induce tyrosine phosphorylation of cytoplasmic proteins upon surface clustering (Freedman et al., 1993), and to participate in gene induction (Yurochko et al., 1992). In particular, a direct comparison of beta 1 and beta 5 cytoplasmic domains using a chimeric beta 1-beta 5 construct where the cytoplasmic domain of beta 1 was replaced with that of beta 5 has demonstrated that the beta 5 cytoplasmic domain, unlike the beta 1 counterpart, does not efficiently direct integrins to the focal contact or promote cell proliferation (Pasqualini and Hemler, 1994). Recently identified beta 3-endonexin (Shattil et al., 1995) and cytohesin-1 (Kolanus et al., 1996), which display restricted binding to the beta 3 and beta 2 cytoplasmic domain, respectively, suggest the presence of proteins that can discriminate the subtle differences in the amino acid sequence of different beta  subunits. These beta  subunit cytoplasmic domain-specific binding proteins may allow specific regulation of individual beta  subunits.

In the present study, we report a novel polypeptide named ICAP-1alpha (integrin cytoplasmic domain-associated protein-1) that binds to the beta 1 integrin cytoplasmic domain. The interaction, which can be demonstrated both in vitro and in vivo, is specific for the beta 1 integrins and requires the Asn and Tyr residues of the membrane-distal NPXY motif. The ability of ICAP-1alpha to interact only with the beta 1 cytoplasmic domain is attributed to an additional requirement of Val residue NH2-terminal to the NPXY. The functional role of ICAP-1 in cell adhesion is suggested by the observation that ICAP-1alpha is a phosphoprotein and that the degree of phosphorylation is regulated by integrin-dependent, cell-matrix interaction.


Materials and Methods

Cell Lines and Antibodies

293, HeLa, Jurkat, K562, and Cos-7 cells were obtained from American Type Culture Collection (Rockville, MD). SaOS, Rat-1, NIH3T3, 2f-TGH (human fibroblast cells), and UTA-6 (Englert et al., 1995) were gifts from C. Sawyers (University of California, Los Angeles, CA [UCLA]), K. Shuai (UCLA), and D. Haber (Massachusetts General Hospital, Boston, MA).

Cells expressing constitutively activated RhoA were generated using UTA-6 (Englert et al., 1995), a derivative of U2OS cells (osteosarcoma cell line) expressing tetracycline-repressible transactivator (Gossen and Bujard, 1992). An NH2-terminal FLAG epitope-tagged RhoA(Q63L) (Coso et al., 1995) was cloned into pTPH-1 (Gossen and Bujard, 1992). The expression construct was introduced into UTA-6 cells using a Ca2PO4-precipitation method (Ausubel et al., 1994) and hygromycin- resistant clones were isolated in the presence of 1 µg/ml tetracycline.

An anti-beta 1 integrin mAb producing hybridoma cell line, TS2/16, was generously provided by M. Hemler (Dana Farber Cancer Institute, Boston, MA). A mouse hybridoma cell line TS1/18 producing anti-beta 1 integrin mAb were obtained from ATCC. Hybridoma cell lines were cultured in DME + 10% CPSR-3 (Sigma Chemical Co., St. Louis, MO). mAb from tissue culture supernatant was purified on protein A-Sepharose (Pharmacia LKB Biotechnology, Inc., Piscataway, NJ).

Polyclonal rabbit antisera against the alpha  subunit of LFA-1 (alpha Lbeta 2) were prepared by immunizing rabbits with bacterially expressed glutathione-S-transferase (GST) fusion protein containing the entire 58-amino acid (aa) cytoplasmic domain (aa 1,088-1,145). For the generation of rabbit antisera against ICAP-1, the entire ICAP-1alpha coding sequences were cloned in pET16b (Novagen Inc., Madison, WI) and expressed as a histidine-tagged protein in Escherichia coli DE21 (Studier and Moffatt, 1986). His-tagged ICAP-1alpha was purified on nickel charged column (Novagen Inc.) under denaturing conditions following the manufacturer's recommendations and used as the immunogen.

Yeast Genetic Screening

The yeast genetic screening for the isolation of proteins interacting with the cytoplasmic domain of beta 1 integrin was carried out essentially as described previously (Gyuris et al., 1993). COOH-terminal 21 aa (GENPIYKSAVTTVVNPXYEGK) of the beta 1 subunit was cloned in frame into LexA coding sequence to generate a "bait" plasmid pNlex-beta 1cyto. The resulting Lex-beta 1cyto fusion protein was able to bind LexA operator in yeast, but displayed no basal transcriptional activity. A yeast expression library was generated from oligo dT-primed cDNA from JY cell (human B cell line) mRNA. The cDNA was cloned unidirectionally into the EcoRI/ XhoI sites of a yeast expression vector pJG4-5. This cDNA library that had the complexity of >106 was amplified once and used to transform a yeast strain EGY48 harboring pNlex-beta 1cyto and JK103 lacZ reporter plasmid. Approximately 2 × 106 independent yeast transformants were pooled and subjected to selection as described. Eight positive clones obtained all had identical cDNA insert. Plasmid DNA from one isolate (Clone E16-1) was rescued using E. coli KC8 (Gyuris et al., 1993) and amplified for further analysis. All bait constructs containing various integrin cytoplasmic domains or beta 1 integrin cytoplasmic domain mutants were tested for proper expression in a "suppression assay" in yeast using a reporter construct JK101 (Gyuris et al., 1993). beta -galactosidase activity measurement was carried out as described previously (Ausubel, 1994).

Northern Blot Analysis and cDNA Cloning

The cDNA insert from Clone E16-1 was used as the probe to screen a multiple tissue mRNA blot (Clontech, Palo Alto, CA). A full-length cDNA was isolated from a HeLa cell cDNA library (a gift from K. Shuai, UCLA) using Clone E16-1 as the probe. An expressed sequence tag (EST) clone corresponding to ICAP-1beta was obtained from the IMAGE Consortium (these sequence data are available EMBL/GenBank/DDBJ under accession number T69975). The presence of cDNA corresponding to ICAP-1beta was independently confirmed by PCR (40 cycles: 94°C for 45 s; 55°C for 45 s; 72°C for 30 s) using primers A3 (5'-CCCAGCAAGATGGAAAGTTGCC-3') and B2 (5'-GATCAGCATTTTACACAATCCA-3') flanking the deleted sequences, which generated a 459- (ICAP-1alpha ) or a shorter 308-bp fragment (ICAP-1beta ).

In Vitro Interaction Assay

For in vitro GST "pull-down" experiments, the COOH-terminal cytoplasmic domains of beta 1 subunit (see above), beta 2 subunit (DNPLFKSATTTVMNPKFAES), and alpha L (KVGFFKRNLKEKMEAGRGVPNGIPAEDSEQLASGQEAGDPGCLKPLHEKDSESGGGD) were individually ex-pressed in bacteria as GST fusion proteins. The EcoRI/XhoI insert of Clone E16-1 encoding ICAP-1alpha (aa 54-200) was cloned into the EcoRI/ XhoI site of an in vitro transcription vector pCITE-3a (Novagen, Inc.). The resulting pCITE-ICAP1alpha plasmid was used as the template in cotranscription/translation reaction using T7 RNA polymerase and rabbit reticulocyte lysate (Promega Corp., Madison, WI) to generate [35S]methionine-labeled polypeptides. An equal amount of labeled polypeptides was added to ~2 µg of GST fusion proteins bound on glutathione-Sepharose beads (Pharmacia LKB Biotechnology Inc.) and incubated overnight at 4°C in NET (25 mM Tris-HCl, pH 7.6, 100 mM NaCl, 3 mM EDTA) containing 1 mM DTT, 1% BSA, and 0.1% Triton X-100. Beads were washed twice in the binding buffer and twice in 0.05% Triton X-100 in NET. Bound proteins were eluted by boiling in SDS sample buffer and analyzed by SDS gel electrophoresis.

Eukaryotic Expression Plasmids

The coding sequences of human alpha L integrin (CD11a) and human beta 2 (CD18) from the expression vectors previously described (Hibbs et al., 1991) were cloned into the XbaI site of the eukaryotic expression vector pcDNA3 (Invitrogen Inc., Madison, WI). To construct a hybrid beta 2.1 subunit, two PCR-generated fragments corresponding to the amino acids 634-749 of beta 2 and amino acids 778-798 of beta 1 were ligated together using a XcmI site that was introduced during the PCR. This fragment was cloned into the BstBI and NotI site of pcDNA3/beta 2 to generate pcDNA/beta 2.1. The sequence of the cytoplasmic domain in this hybrid beta 2.1 subunit consists of NH3-KALIHLSDLREYRRFEKEKLKSQWNGENPIYKSAVTTVV- NPXYEGK-COOH.

The full-length ICAP-1alpha cDNA was cloned into the EcoRV site of pcDNA3 to generate pcDNA/ICAP-1alpha . To generate the full-length ICAP-1beta coding sequence, the 5' half of the above PCR fragment and the 3' half of a second PCR fragment amplified from T69975 clone (see above) were ligated in frame using a unique HpaII restriction site. The ligation product was cloned into the EcoRV site of pcDNA3 to produce pcDNA/ICAP-1beta . The GST-tagged ICAP-1 constructs were generated using eukaryotic expression vector pEBG. The coding sequence of the partial ICAP-1alpha (aa 54-200) was derived from Clone E16-1.

Eukaryotic Expression and In Vivo Interaction Assay

5-10 µg of plasmid DNA was transfected into 293T cells by using a Ca2PO4 precipitation method (Ausubel et al., 1994). 48 h after transfection, cells were lysed in TBSM (25 mM Tris-HCl, pH 7.6, 150 mM NaCl, and 2 mM MgCl2) containing 0.5% NP-40, leupeptin, aprotinin, and PMSF. Detergent insoluble materials were removed by centrifugation at 12,000 g for 15 min. 500 µg of cleared lysates were mixed with an equal volume of TBSM to reduce the final detergent concentration to 0.25% NP-40 and incubated with glutathione-Sepharose beads for 3 h at 4°C. Beads were washed with TBSM + 0.25% NP-40 once, TBSM + 0.1% NP-40 twice, and TBSM alone twice. Coprecipitation of beta 1 integrins with bound GST-ICAP1 was determined on a Western analysis using mAb TS2/16 (anti-beta 1 integrin).

In Vitro Phosphatase Assay

50 µg NP-40 detergent lysate in 40 µl of 25 mM Tris-HCl, pH 8.0, 50 mM NaCl, and 10 mM MgCl2, was incubated at 30°C for 30 min, either in the presence or absence of phosphatase inhibitors (1 mM NaVO3 and 0.5 µM calyculin A). The reaction was terminated by adding SDS sample buffer and boiling for 5 min. 15 µg of lysates were analyzed on Western blot using anti-ICAP1 antibody.

Adhesion Assay

Cell adhesion was carried out using six-well tissue culture plates. Each 35-mm well was coated with 1 ml of fibronectin (20 µg/ml) or poly-L-lysine (PLK) (50 µg/ml) in TBS (25 mM Tris-HCl, pH 8.0, 150 mM NaCl) overnight at 37°C. The coated wells were subsequently blocked with 1% BSA (Faction V; GIBCO BRL, Gaithersburg, MD) in TBS before the addition of cells. Adhesion was carried out using 5 × 105 cells per well at 37°C for 15-30 min and bound cells were lysed in 0.2% SDS in TE (25 mM Tris-HCl, pH 8.0, 1 mM EDTA).


Results

Cloning of ICAP-1

A genetic screening based on the protein-protein interaction in yeast (Gyuris et al., 1993) was used to identify polypeptides that interact with the beta 1 integrin cytoplasmic domain. Of the 47 aa that comprise the entire beta 1 integrin cytoplasmic domain, the COOH-terminal 21 aa were cloned in frame into the DNA-binding domain of bacterial protein LexA. Mutational studies and analysis of naturally occurring splicing variants lacking this 21-aa region have shown that this region is involved in the localization of beta 1 integrins to the focal contacts and proper adhesive function of integrins (Balzac et al., 1993; Meredith et al., 1995).

Using the COOH-terminal 21 aa of integrin beta 1 as the bait, a ~1.1-kb partial cDNA was isolated in a yeast two-hybrid screening of a human B cell cDNA library. This partial cDNA insert was used to obtain a full-length cDNA encoding a polypeptide of 200 aa. In a yeast two-hybrid assay, both the original partial cDNA (aa 154-200) and the full-length cDNA exhibited comparable interaction with the beta 1 integrin cytoplasmic domain but not with unrelated baits (Table I). In addition, the partial or full-length cDNA clones interacted with baits containing either the beta 1 integrin COOH-terminal 21 aa or a longer 47 aa that comprise the complete cytoplasmic domain. The interaction was specific towards only the beta 1 integrin as neither the partial nor the full-length cDNA clone interacted with the beta 2, beta 3, or beta 5 integrin cytoplasmic domain. The isolated clone hereon will be referred as ICAP-1alpha .

Table I. ICAP1: Integrin Cytoplasmic Domain Interaction in Yeast Genetic Screen

[View Table]

The deduced amino acid sequence of ICAP-1alpha was unrevealing except for the preponderance (especially in the NH2-terminal region) of Ser and Thr, several of which represented potential phosphorylation sites (Fig. 1 A). In particular, Ser20, Ser46, and Ser197 represent potential phosphorylation sites by protein kinase C (Woodgett et al., 1986), and Ser10 is present in sequence context favorable for phosphorylation by cyclic nucleotide dependent protein kinases (Glass et al., 1986). The initiation codon ATG in the sequence was preceded by an in frame termination codon and was present in correct sequence context for translational initiation (Kozak, 1992). Although clones corresponding to the ICAP-1alpha cDNA were represented in the National Center for Biotechnology Information (NCBI) EST database, analyses of the NCBI Non-Redundant database using the BLAST Enhanced Alignment Utility algorithm failed to identify any significant similarities to known proteins.



Fig. 1. ICAP-1alpha is a novel serine/threonine-rich 20-kD protein. (A) Sequence of ICAP-1. The translated amino acid sequence of ICAP-1alpha is shown. 50 amino acids absent in ICAP-1beta are indicated in underlined italics. The nucleotide sequence data are available from GenBank/EMBL/DDBJ under accession number AF012023. (B) Northern blot analysis of ICAP-1alpha . Northern blots of transcripts from various human tissues (Clontech) were hybridized with ICAP-1alpha cDNA probe. The 0.9-kb mRNA is expressed in all eight tissue samples. The minor 1.3-kb mRNA, derived from the use of the downstream polyadenylation site, is also expressed in varying amount in all eight samples. (C) Western blot analysis of ICAP-1. ICAP-1alpha and ICAP-1beta were detected in the total cell lysate (5 µg) from 293T cells using polyclonal anti-ICAP-1 antibody (lane 1). The assignments of ICAP-1alpha and ICAP-1beta were confirmed by transiently expressing ICAP-1alpha (lane 2) and ICAP-1beta cDNA (lane 3) in 293T cells. (D) The full-length 200-aa ICAP-1alpha and an alternatively spliced ICAP-1beta lacking internal 50 aa are shown in a schematic diagram.
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In Northern analyses, ICAP-1alpha transcripts of 0.9 and 1.3 kb in size were detected in mRNA isolated from several tissues (Fig. 1 B). The beta 1 integrins are ubiquitously expressed and ICAP-1, as a beta 1 integrin-binding protein, is expected to have a similar broad expression in various tissues and cell lines. The differences in the size of two transcripts probably reflect the usage of two different polyadenylation sites observed during cDNA sequence analysis.

In Western analyses using polyclonal anti-ICAP1 antibodies, two distinct polypeptides of 20 and 16 kd were detected (Fig. 1 C, lane 1). In hypotonic cell lysis, ICAP-1alpha fractionates in the cytosolic fraction suggesting ICAP-1alpha is a cytoplasmic protein (data not shown). Expression of ICAP-1alpha cDNA in 293T cells produced the exact 20-kD polypeptides (Fig. 1 C, lane 2). The shorter 16-kD species likely represent ICAP-1beta , an alternatively spliced variant of ICAP-1alpha lacking internal 50 aa (Fig. 1 D). A cDNA corresponding to ICAP-1beta mRNA was recognized during the database search. The presence of ICAP-1beta cDNA in several human cDNA libraries was subsequently confirmed by PCR analyses (data not shown). The expression of ICAP-1beta cDNA in 293T cells, as expected, produced the exact 16-kD polypeptide that comigrated with the endogenous ICAP-1beta polypeptides (Fig. 1 C, lane 3). Interestingly, ICAP-1beta did not interact with the integrin beta 1 cytoplasmic domain in a yeast two-hybrid assay (data not shown).

beta 1 Integrin Cytoplasmic Domain Restricted Binding of ICAP-1alpha

To verify the specific interaction between ICAP-1alpha and the beta 1 integrin cytoplasmic domain seen in the yeast genetic screening, in vitro-translated ICAP-1alpha was incubated with various integrin cytoplasmic domains expressed in bacteria as GST fusion proteins (Fig. 2 A). After incubation, GST fusion proteins were isolated on glutathione-Sepharose beads and the bound ICAP-1alpha polypeptides were analyzed by a SDS gel electrophoresis. As in a yeast two-hybrid assay, ICAP-1alpha was bound only to the GST-beta 1 fusion protein and not to the GST-beta 2 or GST-alpha L fusion proteins.


Fig. 2. In vitro and in vivo interaction between ICAP-1alpha and beta 1 integrins. (A) Interaction between GST-beta 1 and ICAP-1alpha in vitro. ICAP-1alpha (aa 54-200) was synthesized in vitro using reticulocyte lysate (lane 1) and incubated with 2 µg of bacterially expressed GST fusion proteins containing the cytoplasmic domains of integrin beta 1 (lane 2), beta 2 (lane 3), and alpha L (lane 4). (B) Interaction between GST-ICAP1alpha and beta 1 integrins in vivo. ICAP-1alpha (aa 54- 200) (lane 1) and a full-length ICAP1alpha (lane 2) were expressed as GST fusion protein in 293T cells using eukaryotic expression vector pEBG. For controls, GST-Stat1 (lane 3) and GST (lane 4) were used. Coprecipitation of the endogenous beta 1 integrins with the GST fusion proteins were determined on a Western blot using TS2/16 (anti-human beta 1 integrin antibody). (C) Restricted binding specificity of ICAP1alpha . GST-ICAP1alpha was expressed in 293T cells along with expression constructs for alpha Lbeta 2 or alpha Lbeta 2-1 (chimeric beta 2 subunit that has the COOH-terminal 20 aa replaced with the COOH-terminal 21 aa of beta 1 integrin). Coprecipitation of transfected integrins with the GST-ICAP1alpha was determined on a Western blot using anti-alpha L antibody. Lanes 1 and 2, total lysates of cells expressing GST-ICAP1alpha and alpha Lbeta 2 (lane 1) and alpha Lbeta 2-1 (lane 2). Lanes 3 and 4, the same samples as in lanes 1 and 2, respectively, after GST "pull-down."
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Reciprocal GST pull-down experiments were used to demonstrate the interaction between ICAP-1alpha and endogenous beta 1 integrins in vivo. GST epitope-tagged ICAP-1alpha was transiently expressed in 293T cells. Glutathione- Sepharose beads were used to purified GST-ICAP1alpha and copurification of endogenous beta 1 integrins was tested on a Western analysis using anti-beta 1 antibody. Confirming the results of the yeast two-hybrid assays and in vitro binding studies, beta 1 integrins copurified with GST-ICAP1alpha , but not with unrelated GST fusion proteins or GST alone (Fig. 2 B).

Finally, to demonstrate that ICAP-1alpha is a specific beta 1 integrin binding protein in vivo, GST epitope-tagged ICAP-1alpha was coexpressed in 293T cells with the integrin beta 2 subunit or a chimeric beta 2 subunit (beta 2.1) constructed by replacing the COOH-terminal 20 aa region of the beta 2 subunit with the corresponding sequences of the beta 1 subunit. The expression of beta 2 integrins is limited to cells of hematopoietic origin and 293T cells completely lack expression of beta 2 integrins (Liu, J., and D. Chang, unpublished observation). To ensure a proper cell surface expression, the alpha L subunit capable of forming stable heterodimer with the beta 2 subunit was also expressed. A comparable surface expression of alpha Lbeta 2 and alpha Lbeta 2.1 was seen on FACS® analysis (data not shown). After precipitation of GST-ICAP1alpha on glutathione-Sepharose beads, copurification of beta 2 integrin was indirectly tested in Western analysis using anti-alpha L antibody. In GST pull-down experiments, as predicted from the yeast two-hybrid assays and in vitro binding assays, only alpha Lbeta 2.1 and not alpha Lbeta 2 copurified with ICAP-1alpha (Fig. 2 C). These findings demonstrate the ability of ICAP-1alpha to interact with integrin alpha beta heterodimer in a beta 1 cytoplasmic domain-dependent fashion.

Conserved NPXY Motif of Integrin beta 1 Cytoplasmic Domain is Required for the ICAP-1alpha Binding

ICAP-1alpha interacts with the beta 1 integrins through the COOH-terminal 21 aa region. Naturally occurring, alternatively spliced variants of beta 1 integrins, which lack this region or mutant beta 1 integrins containing amino acid substitutions at either of the two conserved NPXY motifs do not localize to the focal contacts (Reszka et al., 1992; Balzac et al., 1993). Furthermore, mutations in the analogous region of beta 2 or beta 3 integrins affect the leukocyte integrin alpha Lbeta 2 (LFA-1)-dependent cell adhesion to intercellular cell adhesion molecules-1 or the affinity regulation of the platelet integrin alpha IIbbeta 3 (Hibbs et al., 1991; O'Toole et al., 1995; Peter and O'Toole, 1995).

The exact ICAP-1alpha binding site within the beta 1 integrin cytoplasmic domain was further delineated by testing the interaction between ICAP-1alpha and truncated beta 1 integrin cytoplasmic domain (Table II). NH2-terminal deletions up to 8 aa did not affect the interaction with ICAP-1alpha . A 3-aa deletion in the COOH terminus, however, completely abolished the interaction. Therefore, a minimum binding site for ICAP-1alpha on the beta 1 integrin cytoplasmic must consist of this 13-aa region that included one of the two NPXY motifs. The NPXY motif is required for the interaction as amino acid substitutions at the conserved Asn to Glu or Ala abolished the binding. Similarly, a substitution of Tyr to Ala disrupted the ICAP-1alpha binding whereas a more conservative replacement of Tyr to Phe had no effect.

Table II. ICAP1: beta 1 Subunit Deletion Mutant Interaction

[View Table]

In yeast interaction and in vitro binding assays, ICAP-1alpha failed to interact with the beta 2 integrin cytoplasmic domain, which is closely related to the beta 1 integrin cytoplasmic domain in sequence (Table III). The molecular basis of this highly discriminatory binding specificity of ICAP-1alpha was investigated by progressively mutating the beta 2 integrin cytoplasmic domain. There are two conserved and four nonconserved amino acid differences between the beta 1 and beta 2 integrins within the minimum 13 aa ICAP-1alpha binding region. A replacement of three consecutive nonconserved residues found in the COOH terminus of beta 2 integrin from Ala-Glu-Ser to Glu-Gly-Lys found in the beta 1 subunit did not allow the ICAP-1alpha binding. Interestingly, a single amino acid replacement at the -11 position from Thr to Val allowed the mutant beta 2 integrin to interact with ICAP-1alpha , demonstrating that in addition to the NPXY motif, Val at the -11 position is essential for the interaction between beta 1 integrins and ICAP-1alpha .

Table III. ICAP1: beta 1 and beta 2 Subunit Point Mutant Interaction

[View Table]

ICAP-1alpha Is a Phosphoprotein

In addition to the 20- and 16-kD polypeptides corresponding to ICAP-1alpha and ICAP-1beta , heterogeneous bands migrating slower than the 20-kD band were detected in Western analysis of 293T cell lysates using polyclonal anti-ICAP1 antisera (data not shown). Because the amino acid composition of ICAP-1 is rich in Ser and Thr, the possibility that this mobility difference is due to protein phosphorylation was raised. When total detergent lysates of several different cell lines were surveyed by Western analysis, there was a significant variation in the relative amount of slow migrating species (Fig. 3 A). In particular, two osteosarcoma cell lines, SaOS (Fig. 3 A, lane 2) and UTA-6 (Fig. 3 A, lane 3), displayed high abundance of the slow migrating species. To directly confirm that the slow migrating species represented phosphorylated forms of ICAP-1alpha , UTA-6 cell lysates incubated at 30°C to activate endogenous phosphatases (Fig. 3 B). The slow migrating species disappeared with a concomitant increase in the 20 kD ICAP-1alpha upon 30°C incubation (Fig. 3 B, lane 3). The addition of phosphatase inhibitors, sodium vanadate, and calyculin A, completely prevented the conversion of the slow migrating species to the 20 kD ICAP-1alpha (Fig. 3 B, lanes 2 and 4), confirming the mobility difference is due to protein phosphorylation. Interestingly, during in vitro phosphatase experiment, we failed to detect any mobility shift in ICAP-1beta , suggesting that either ICAP-1beta is not phosphorylated or the mobility of ICAP-1beta does not change significantly by protein phosphorylation.


Fig. 3. ICAP-1alpha is a phosphoprotein. (A) Western blot analysis showing ICAP-1 expression. Total cell lysates (15 µg) from various cell lines were analyzed on a Western blot using anti-ICAP1 antibody. The positions of ICAP-1alpha and ICAP-1beta as well as the slow migrating species corresponding to the phosphorylated ICAP-1alpha (p-ICAP1alpha ) are indicated. (B) In vitro phosphatase treatment experiment. 15 µg of UTA-6 cell lysates were incubated at 30°C for 30 min. Lane 1, input; lane 2, incubation in the presence of sodium vanadate and calyculin A; lane 3, incubation at 30°C.
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Regulation of ICAP-1alpha Phosphorylation by Integrin-dependent Cell-Matrix Interaction

Many focal contact proteins, FAK, paxillin, and tensin being a few representative ones, have been shown to undergo protein phosphorylation during cell attachment (Clark and Brugge, 1995). The possibility that the phosphorylation of ICAP-1alpha may be regulated in a similar manner during cell attachment was directly addressed by binding UTA-6 cells to either PLK or fibronectin (FN)- coated surface (Fig. 4). Western analyses of cell lysates revealed an enhanced phosphorylation of ICAP-1alpha when cells adhere to the FN-coated surface. The maximum enhancement occurred within the minimum 30 min required for sufficient number of cells to adhere and undergo cell spreading (Fig. 4, lanes 2 and 4). A longer incubation did not further enhance ICAP-1alpha phosphorylation (data not shown). This enhancement was specific for cell attachment to FN-coated surface, which requires beta 1 integrins. On PLK-coated surface, cells adhere very efficiently, but fail to initiate subsequent cell spreading. Under these conditions, the phosphorylation of ICAP-1alpha remained at a reduced level (Fig. 4, lanes 1 and 3).


Fig. 4. Regulation of ICAP-1alpha phosphorylation during cell adhesion. UTA-6 cells were trypsinized and replated on plates coated with poly-L-lysine (PLK) (lanes 1 and 3) or fibronectin (FN) (lanes 2 and 4). Adherent cells were lysed in 0.5% NP-40 at t = 15 min (lanes 1 and 2), and t = 30 min (lanes 3 and 4) and the extent of ICAP-1alpha phosphorylation was determined on a Western blot using anti-ICAP-1 antibody.
[View Larger Version of this Image (43K GIF file)]

During integrin-dependent cell adhesion, the rearrangement of cytoskeletal actin stress fibers and assembly of focal adhesion plaques are regulated by Rho-family GTPases (Hall, 1994; Burridge and Chrzanowska-Wodnicka, 1996). In UTA-6 derivative cell lines expressing constitutively activated RhoA(Q63L) under the control of tetracycline- inducible system, the induction of RhoA(Q63L) expression results in the formation of dense stress fibers and increase in the number of focal adhesion plaques (Wong, C., and D. Chang, manuscript in preparation). These cells display a significantly delayed and incomplete cell spreading when plated on FN-coated surface (Fig. 5 A). To test whether the alteration in cell-matrix interaction induced by the expression of RhoA(Q63L) had any effect on the phosphorylation status of ICAP-1alpha , a Western analysis was carried out using the lysates from RhoA(Q63L) expressing cells. The lysates prepared from cells grown in the presence of tetracycline, which represses the expression of RhoA(Q63L), were used as controls. Fig. 5 B shows that the expression of constitutively activated RhoA(Q63L) reduces the extent of ICAP-1alpha phosphorylation in two independently derived cell lines. Thus, taken together, these findings suggest that phosphorylation of ICAP-1alpha is regulated during integrin-dependent cell adhesion and spreading.



Fig. 5. Control of ICAP-1alpha phosphorylation by RhoA protein. (A) Expression of activated RhoA interferes with cell spreading. The morphology of UTA-6 cells expressing RhoA(Q63L) under the control of tetracycline-inducible promoter is shown. In the absence of tetracycline, which induces the expression of RhoA (Q63L), cells remain rounded and fail to spread. (B) Expression of activated RhoA results in dephosphorylation of ICAP-1alpha . The extent of ICAP-1alpha phosphorylation in two independently derived UTA-6 cell lines expressing RhoA (Q63L) was assessed in Western blot analysis using anti-ICAP1 antibody. In parental cells (lane 1) or in the presence of tetracycline (lanes 2 and 4), a significant portion of ICAP-1alpha is phosphorylated. Induction of RhoA(Q63L) is associated with the loss of ICAP-1alpha phosphorylation is both cell lines (lanes 3 and 5).
[View Larger Versions of these Images (89 + 24K GIF file)]


Discussion

Integrins, through binding to both extracellular matrix proteins and cytoskeletal structures, provide a direct linkage between the extracellular environment and the cell interior. The cytoplasmic domains of integrins have been implicated in the integrin affinity regulation and localization of integrins to the focal contacts. Understanding how the short cytoplasmic tails of integrins affect the functions of integrins requires characterization of cellular proteins that bind, either directly or indirectly, to the integrin cytoplasmic domains. To this end, we have used a yeast two-hybrid screen to identify proteins that directly bind to the beta 1 integrin cytoplasmic domain. In particular, we have focused on the COOH-terminal 21-aa region of the beta 1 integrin, which is required for the localization of integrins to the focal contacts. In this study, we report identification and characterization of ICAP-1alpha , a novel 200-aa protein that specifically interacts with the beta 1 integrin cytoplasmic domain through a conserved and functionally important NPXY sequence motif. In addition, ICAP-1alpha undergoes protein phosphorylation that is subject to regulation, suggesting that ICAP-1alpha plays an important role during integrin-dependent cell adhesion.

The broad tissue distribution of ICAP-1alpha mRNA and the detection of ICAP-1alpha protein in cell lines of various tissue origins indicate ICAP-1alpha , like beta 1 integrins, is ubiquitously expressed. In contrast, the expression of ICAP-1beta , an alternatively spliced variant of ICAP-1alpha that does not bind to the beta 1 integrin cytoplasmic domain, was more variable in cell lines we have tested. In particular, the ICAP-1beta expression was low or absent in three cell lines, SaOS (human osteosarcoma line), TPH-1 (human monocytic line), and Cos-7 (monkey kidney line). The differences in the ability to interact with the beta 1 cytoplasmic domain and in the expression level provide a mechanism for regulation of ICAP-1alpha function by ICAP-1beta . It is noteworthy that beta 3-endonexin likewise has an alternatively spliced variant that does not interact with the beta 3 integrin cytoplasmic domain (Shattil et al., 1995).

The amino acid sequence of ICAP-1alpha is unique and displays no similarities to any known proteins. Several proteins, including known focal contact proteins, alpha -actinin, paxillin, talin, and FAK, as well as recently identified potential regulatory proteins such as beta 3-endonexin, ILK-1, and cytohesin-1 have been shown to bind integrins through the beta  subunit cytoplasmic domain (for review see Sastry and Horwitz, 1993; Shattil et al., 1995; Hannigan et al., 1996; Kolanus et al., 1996). In contrary to alpha -actinin, FAK, and ILK-1, which interact with more than one type of beta  subunit, the interaction of ICAP-1alpha is restricted to the beta 1 subunit only. This property of ICAP-1alpha is similar to two recently identified proteins, beta 3-endonexin and cytohesin-1, which bind specifically to integrins beta 3 and beta 2, respectively (Shattil et al., 1995; Kolanus et al., 1996). As these proteins are not related in sequence to each other or to ICAP-1alpha , it remains to be seen how these unrelated proteins are coupled to the beta  integrin cytoplasmic domains, which are related and functionally interchangeable in some cases.

The results from our mutagenesis studies indicate that the COOH-terminal 13-aa region of the beta 1 subunit, which includes a conserved NPXY motif, is sufficient to bind ICAP-1alpha . Several studies have shown that this region, especially the NPXY motif, is important for the recruitment of beta 1 integrins to the focal contacts (Marcantonio et al., 1990; Reszka et al., 1992; Peter and O'Toole, 1995; Ylanne et al., 1995) and the colocalization of talin, FAK, and actin with beta 1 integrins (Lewis and Schwartz, 1995). Our finding that Asn to Ala (or Glu), or Tyr to Ala substitution within the NPXY motif completely abolished the interaction between ICAP-1alpha and integrins, while a more conservative replacement of Tyr with Phe had no effect, demonstrates a remarkable similarity between the sequence requirement for the binding of ICAP-1alpha to integrins and localization of beta 1 integrins to the focal contacts and suggests that ICAP-1alpha may play a role in the recruitment of beta 1 integrins to the focal contacts. Alternatively, the COOH-terminal 13-aa region may be involved in initiating signal transduction events required to trigger cell spreading and ICAP-1alpha may participate in the initiation of this signaling event. As beta 2, beta 3, and beta 5 integrin cytoplasmic domains (which do not bind ICAP-1alpha ) can also direct integrins to the focal contacts (LaFlamme et al., 1994; Peter and O'Toole, 1995), there must be parallel mechanisms for recruiting various integrins to the focal contacts.

The COOH-terminal regions of different beta  integrins are somewhat diverse in amino acid sequence, which may explain in part the observed specificity of ICAP-1alpha interaction with the beta 1 integrin cytoplasmic domain. The basis of this restricted specificity, however, was attributed to a single Val residue NH2-terminal to the conserved NPXY motif. In beta 2 or beta 3 subunits, the corresponding position is occupied by Thr (Sastry and Horwitz, 1993). Confirming the importance of Val at this position, the replacement of the Thr with a Val in the beta 2 subunit cytoplasmic tail allowed it to bind ICAP-1alpha . The beta 3 integrin cytoplasmic domain, in addition, has a less conserved NITY in place of the NPXY motif, which may also account for the lack of interaction with ICAP-1alpha . A recent study on the amino acid sequence requirement for the interaction between beta 3-endonexin and beta 3 integrin cytoplasmic domain indicated that the NITY motif is critical for the binding specificity (Eigenthaler et al., 1997). Altogether these findings suggest that the COOH-terminal regions of different beta  integrins may constitute specific binding sites for different cytoplasmic proteins. In addition, the existence of integrin beta  subunit specific binding proteins indicates that the functions of individual subunits can be differentially regulated.

The presence of ICAP-1alpha immunoreacting species, which migrated slower than the expected molecular weight of 20 kD predicted from the ICAP-1alpha reading frame suggested that ICAP-1alpha undergoes posttranslational modification. The conversion of slower migrating species to the expected 20-kD species during 30°C incubation, which activates the endogenous phosphatases present in nonionic detergent lysates, and the observation that this conversion can be effectively blocked by the addition of known phosphatase inhibitors demonstrate that ICAP-1alpha is a phosphoprotein.

The most intriguing property of ICAP-1alpha is that its phosphorylation is regulated during cell adhesion and by RhoA protein. We suspect that the effect on ICAP-1alpha phosphorylation seen during cell adhesion on FN-coated surface and in cells expressing constitutively activated RhoA(Q63L) are related events, reflective of the cytoskeletal rearrangement that occurs during cell adhesion. It is well known that cells, soon after making an initial contact on FN-coated surface, undergo cell spreading that involves the formation of focal adhesion plaques and actin stress fibers (for review see Hall, 1994). Both these events are known to be regulated by RhoA (Ridley and Hall, 1992; Nobes and Hall, 1995). Furthermore, both matrix assembly and cell spreading are integrin-dependent processes and require integrin cytoplasmic domain (for review see Burridge and Chrzanowska-Wodnicka, 1996; LaFlamme et al., 1994). On a nonspecific PLK-coated surface, cells make initial contact but fail to promote matrix assembly and initiate spreading. Cells expressing RhoA(Q61L) also display a delayed and incomplete spreading, presumably due to the interference from dense stress fibers. According to this scenario, the diminished ICAP-1alpha phosphorylation observed during cell adhesion on PLK-coated surface and in RhoA(Q63L) expressing cells is a result of inefficient cell spreading. Our findings, however, do not rule out the possibility that phosphorylation of ICAP-1alpha may be a direct consequence of the interaction between integrins and fibronectin. Regardless of the exact mechanism underlying the control of ICAP-1alpha phosphorylation, our data clearly demonstrate that the phosphorylation of ICAP-1alpha is regulated during the cell-matrix interaction.

It remains to be seen whether the enhancement in ICAP-1alpha phosphorylation during cell attachment involves Ser/Thr phosphorylation or Tyr phosphorylation. Although Ser/Thr phosphorylation is suspected based on the amino acid composition of ICAP-1alpha (21% Ser/Thr) and the presence of potential protein kinase C and protein kinase A phosphorylation sites, the exact amino acid residues that are phosphorylated are not known. We have not been able to demonstrate the immunoreactivity of ICAP-1alpha with PY20 anti-phosphotyrosine antibody (data not shown).

In summary, we present initial characterization of ICAP-1alpha , a novel beta 1 integrin cytoplasmic domain binding protein. Two observations, (a) the binding of ICAP-1alpha to a conserved region of beta 1 integrin cytoplasmic domain that previously has been shown to be important for the adhesive function and focal contact localization of integrins, and (b) the extent of ICAP-1alpha phosphorylation is regulated during cell-matrix interaction, suggest that ICAP-1alpha plays a role during integrin-dependent cell adhesion. beta  Integrin cytoplasmic domains likely contain overlapping binding sites for both structural and regulatory proteins that coordinate cell adhesion and subsequent cytoskeletal rearrangement. The identification of ICAP-1alpha together with the characterization of the sequences on beta 1 integrin cytoplasmic domain required for the ICAP-1alpha binding should facilitate future studies on how specific integrin- dependent cellular events are regulated.


Footnotes

Received for publication 23 April 1997 and in revised form 3 July 1997.

   Please address all correspondence to David D. Chang, UCLA School of Medicine, Division of Heme-Onc, Factor 11-934, 10833 Le Conte Avenue, Los Angeles, CA 90095. Tel.: (310) 825-9759. Fax: (310) 825-6192. e-mail: dchang{at}medicine.medsch.ucla.edu

We are grateful to R. Brent (Massachusetts General Hospital, Boston, MA) for providing the yeast strains and plasmids for the yeast genetic screening, to J. Gutkind (National Institutes of Health, Bethesda, MD), for providing mAbs, cell lines, and DNA constructs. We thank T. Kim for technical assistance and K. Shuai for helpful comments on the manuscript.

This work was supported by the grants from Searle Scholars Program/ The Chicago Community Trust, James S. McDonnell Foundation, and Jonnson Comprehensive Cancer Center of UCLA.


Abbreviations used in this paper

aa, amino acid; FAK, focal adhesion molecule; FN, fibronectin; GST, glutathione-S-transferase; ICAP-1, integrin cytoplasmic domain-associated protein-1; PLK, poly-L-lysine.


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