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Article |
Address correspondence to Matthew J. Tyska, Department of Molecular, Cellular, and Developmental Biology, Yale University 342 Kline Biology Tower, 266 Whitney Ave., New Haven, CT 06511. Tel.: (203) 432-3469. Fax: (203) 432-6161. email: matthew.tyska{at}yale.edu
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Abstract |
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Key Words: actin; membrane; lipid raft; microvillus; epithelium
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Introduction |
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We recently investigated the intramicrovillar dynamics of M1A using the BB-expressing cell line LLC-PK1-CL4 (Tyska and Mooseker, 2002). FRAP measurements on GFP-M1A expressed in CL4 cells showed that 80% of the population of M1A exchanges rapidly (< 1 min), suggesting that most of the M1A does not function as a static linker within the MV. These studies also showed that the membrane-binding tail domain of M1A is critical for the steady-state localization and turnover kinetics of M1A within the BB. Based on these data, we predicted that overexpression of this fragment in an IEC line might inhibit the function of M1A by displacing it from the BB, generating dominant negative phenotypes and ultimately providing functional insight.
Here, we demonstrate that the BBs of CACO-2BBE (BBE; Peterson and Mooseker, 1992) cells expressing the dominant negative M1A-tail fragment are missing sucrase isomaltase (SI), a transmembrane disaccharidase known to reside in lipid rafts in the BB membrane (Danielsen, 1995). In vitro, lipid rafts form stable, detergent-resistant membrane (DRM) microdomains (Danielsen and Hansen, 2003). In this report, we provide evidence indicating that M1A is a component of SI-containing DRMs isolated from both BBE cells and rat MV, and further show that these two proteins may interact in a raft complex. The functional significance of this complex is highlighted by expression of the NH2-terminal cytoplasmic domain of SI, which results in lower levels of M1A and a loss of SI from the BB. Together, these studies are the first to assign a specific cellular role to M1A and suggest that this motor is involved in the retention of SI within the BB.
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Results |
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GFP-M1A-tail expression induces SI loss from the BB
Because past studies have implicated M1A in the apical targeting of BB-specific proteins (Fath et al., 1994), we examined GFP-M1A-tail expressing cells for defects in the distribution of apical markers: specifically, the GPI-linked protein, AP, and two type II transmembrane proteins, SI and aminopeptidase N (APN). Inspection of BBE cells expressing GFP-M1A-tail revealed significant perturbations in SI localization (Fig. 3, A and B); SI and GFP-M1A-tail signals appeared mutually exclusive, forming a "lock and key" staining pattern with very little overlap. Cells expressing extremely low levels of GFP-M1A-tail (Fig. 3 A, unbracketed cells) appeared to target SI in a manner similar to the parent line (Fig. 1 C), whereas those expressing higher levels had little or no SI in the BB. This effect was also specific to SI, as distributions of the other apical membrane markers, APN (Fig. 3 C) and AP (Fig. 3 D), appeared normal. Although the expression of SI in parent line BBE cells does appear highly mosaic (Fig. 1 D; Peterson and Mooseker, 1992), the scale of the expression mosaic (variation from cell to cell) is much smaller than the scale of the interruptions we observed due to GFP-M1A-tail expression, where SI localization was perturbed in large fields of cells (Fig. 3, A, B, and D). Because GFP-M1A-tail expression is limited to a small fraction of the cells in these stable lines, Western blotting only revealed a minor reduction in SI levels (10%; not depicted). However, based on the micrographs in Fig. 3, the true reduction in SI level per cell is probably much greater. Potential mechanisms and implications of SI loss from the BB are discussed in greater detail below (see Discussion).
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M1A is a component of SI-containing DRMs from BBE cells
SI is an integral membrane disaccharidase that is known to reside in lipid rafts of the BB membrane (Danielsen, 1995). If M1A interacts with SI-containing lipid rafts, then expression of the GFP-M1A-tail fragment could result in the loss of SI from the BB by perturbing M1A-dependent transport or localization of raft structures. In vitro, lipid rafts form stable, low density DRMs (Brown and London, 1998). To determine if M1A is a component of SI-containing DRMs, we isolated crude DRMs from fully confluent BBE cells (see Materials and methods; Fig. 4 A, interface) and performed density gradient analysis. The density profiles (Fig. 4 B, dashed line/filled circles) typically generated by the OptiprepTM gradients used for these studies appeared slightly nonlinear, but were highly reproducible. Immunoblots (Fig. 4 C) of gradient fractions showed that SI and M1A are both enriched in the lowest density (1.11 g/ml) fraction. The cholesterol profile (Fig. 4 B, solid line/filled squares) of the same gradient revealed that the lowest density fractions also have the highest cholesterol levels, as expected for DRMs (Brown and London, 1998).
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M1A is a component of native SI-containing DRMs isolated from rat MV
In an effort to confirm the presence of M1A in SI-containing DRM fractions from native intestinal epithelia, we proceeded to isolate DRMs from rat intestinal MV (Fig. 6 A; Mooseker et al., 1989). Light microscopy on ConA/phalloidin costained samples (Fig. 6 B) and EM on negatively stained preparations (Fig. 6 C) enabled us to verify that MV were purified with apical membranes intact. To isolate DRMs, MV were first demembranated with cold 1% Triton X-100 (see Materials and methods). Immunoblots of the fraction solubilized during this treatment (Fig. 6 D, TX100 S; Table I) showed that a significant portion (60%) of microvillar SI was released with cold Triton X-100, a finding consistent with previous reports (Danielsen, 1995). This implies either that many of the rafts present at the time of detergent treatment do not "survive" the extraction or that SI exists in equilibrium between raft- and nonraft-associated forms in the microvillar membrane. Demembranated microvillar cores were then resuspended in saturating ATP to release M1A and any associated complexes (e.g., DRMs). M1A in the resulting "ATP release supernatant (S)" fraction was enriched 2.6-fold relative to intact MV (Fig. 6 E; Table I). To determine if M1A in this fraction was present on low density, SI-containing DRMs, we performed density gradient analysis. M1A was distributed throughout the gradient in both low and high density fractions, suggesting that the ATP release S input contained both DRM- and non-DRMassociated (soluble) M1A populations. Densitometric analysis of the immunoblots (Fig. 6 G) revealed that a significant portion (
14%; Table I) of the total M1A input was found in the lowest density fraction that was enriched in SI (Fig. 6, F and G, Fraction 2,
1.12 g/ml). Because the ATP release S gradient input contains
76% of the total microvillar M1A (Table I), this translates into a value of
10% for the amount of M1A that is associated with lowest density DRMs in the MV. However, 10% is a conservative estimate given that a large fraction of SI tails into higher densities (Fig. 6, F and G). If we consider all the fractions that have significant levels of SI (Fig. 6, Fractions 27), these also contained
60% of the M1A from the ATP release S input (Table I).
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Expression of the SI cytoplasmic domain induces the loss of SI from the BB
On the cytoplasmic face of the microvillar membrane, the only portion of SI available for cross-linking to M1A is its small NH2-terminal cytoplasmic domain (SINT) consisting of 10 aa (Hunziker et al., 1986). If an interaction between M1A and the SINT (be it direct or indirect) is required for the localization of SI-containing DRMs in the BB, expression of this fragment should result in a phenotype similar to that produced by GFP-M1A-tail. To test this proposal, we expressed SINT fused to YFP in BBE cells and examined its effect on SI localization. BBE cells expressing SINT-YFP exhibited a striking loss of SI from the BB domain (Fig. 9, A and B), an effect similar to that induced by GFP-M1A-tail. This effect was specific to SI as distributions of the other apical membrane markers, APN (Fig. 9 C) and AP (Fig. 9 D), appeared normal. However, in cells expressing high levels of SINT-YFP, we also observed that M1A levels were reduced (Fig. 9 E). Although the effects on M1A were not as pronounced as the impact on SI localization, this result does suggest that the SINT may play a role in docking a fraction of M1A to the microvillar membrane in these cells. The limited effect of SINT-YFP expression on M1A localization is also consistent with our biochemical data on native DRMs, which indicates that as little as
10% of the M1A may be DRM-associated in the MV (Fig. 6 G; Table I).
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Discussion |
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SI is a type-II integral membrane disaccharidase involved in carbohydrate processing in the intestinal lumen. In vivo SI exists in two forms: a heavily glycosylated 260-kD pro-SI peptide that targets to the apical surface and, once exposed to the intestinal lumen, a dimer that is formed from the tryptic cleavage of pro-SI into sucrase and isomaltase subdomains (Hunziker et al., 1986). In the BB membrane, SI resides in lipid rafts; detergent-resistant microdomains that are known to be rich in sphingolipids and cholesterol, as well as GPI-linked and other transmembrane proteins (Brown and London, 1998). Intriguingly, rafts may also play a role in the sorting of Golgi-derived membrane to the apical surface (Ikonen and Simons, 1998). It is tempting to speculate that the loss of SI from the BB in the presence of GFP-M1A-tail or SINT-YFP may be due to a defect in the trafficking of Golgi-derived lipid rafts (Fig. 10 C, model 1). In this scenario, vesicles coming from the Golgi would be transported by dynein along microtubules to the terminal web region, at which point M1A would power actin-based motility up into the BB (Fath et al., 1994). If M1A interacts with SI on the surface of lipid rafts, expression of both dominant negative fragments would inhibit M1A from binding to such vesicles, and thus prevent the apical transport of SI-containing rafts. In support of this proposal, the intestine of developing mouse embryo exhibits supra-nuclear M1A staining that may represent Golgi-associated motor (Skowron and Mooseker, 1999). Moreover, Jacob et al. (2003) have recently reported that exogenously expressed SI and a myosin identified as M1A, coimmunoprecipitate from a Golgi-enriched vesicle fraction derived from MDCK cells. However, it is unlikely that the Golgi-associated myosin-I immunogen in these studies is actually M1A. Immunoblots of kidney tissue (Skowron et al., 1998) and MDCK cells using our antibody failed to detect M1A although both do express multiple CX-1 reactive immunogens (unpublished data).
The preponderance of evidence indicates that M1A does not power the apical transport of SI-containing vesicles in IECs. The association of M1A with the Golgi, alluded to above, is only observed in undifferentiated enterocytes (Fath et al., 1994; Skowron et al., 1998), not in differentiated cells of the villus, where synthesis and apical transport of SI must occur given the turnover rates for BB disaccharidases (James et al., 1971). Moreover, in BBE cells expressing GFP-tagged full-length M1A, this motor does not label a population of moving vesicles (unpublished data). The proposed role of the MV core rootlets as tracks for vesicle transport, through the terminal web to the plasma membrane, is also unlikely given that these structures are coated with tropomyosin (Bretscher and Weber, 1978), an actin-binding protein known to inhibit the activity of M1A (Fanning et al., 1994) and other class I myosins (Tang and Ostap, 2001). Tropomyosin also coats the stable actin bundles found in the stress fibers that are commonly observed in unpolarized cells (Tang and Ostap, 2001). Thus, the recently reported movement of SI-containing vesicles along stress fibers (Jacob et al., 2003) is most likely powered by a nonclass I myosin. Finally, the localization of APN (a type II transmembrane raft component; (Danielsen, 1995) and AP (a GPI-linked protein that resides in the same lipid rafts as SI; Garcia et al., 1993), is normal in BBE cells expressing GFP-M1A-tail and SINT-YFP (Figs. 3 and 9). Although it remains possible that APN and AP utilize sorting mechanisms distinct from SI (e.g., M1A independent vs. dependent) to arrive at the apical domain, all of these data together suggest that M1A participates in a process other than motor-driven apical transport.
We propose that M1A is responsible for the retention of SI within BB, specifically through an interaction with SI-containing lipid rafts (Fig. 10, model 2). This interaction would provide a link from SI to the underlying cytoskeleton, stabilizing its residence and extending its lifetime in the BB membrane. Cytoskeletal interactions are known to be critical for the stable localization of other membrane-associated proteins as well. For example, retention of the Na+/K+ ATPase in the basolateral membrane of enterocytes is known to be dependent on its binding to the underlying meshwork of ankyrin and spectrin (Nelson and Veshnock, 1987). Indeed, previous reports have shown that with respect to Na+/K+ ATPase localization, phenotypes associated with ß-spectrin mutations in Drosophila (Dubreuil et al., 2000) are similar to those observed in this work. Here, we observed that the loss of cytoskeletal linkages (induced by expression of GFP-M1A-tail or SINT-YFP, Figs. 3 and 9, respectively) results in a profound loss of SI from the BB with little redistribution to other subcellular compartments. In the absence of cytoskeletal linkages, SI may be more susceptible to removal from the cell via the continuous apical membrane shedding that occurs at microvillar tips in BBE cells (that is, SI is physically lost from the cells). Alternatively, disruption of cytoskeletal linkages may leave SI more susceptible to retrieval from the BB via constitutive endocytic pathways. Once internalized, mislocalized SI would experience a higher rate of degradation. Both of these scenarios could lead to the reduced steady-state SI levels and significantly lower staining intensities observed here.
Are the low duty ratio kinetics (Jontes et al., 1997) and rapid turnover dynamics (Tyska and Mooseker, 2002) reported for M1A consistent with a role in microvillar retention? The unloaded duty ratio of M1A is only 0.1 (Jontes et al., 1997), suggesting that many molecules (at least 10,
1/duty ratio), working together in an ensemble, would be required to form a continuous link between the membrane and the microvillar actin core. One way for M1A to form an ensemble might be through the assembly of lipid rafts in the microvillar membrane. In this scenario, the clustering of n SI molecules in a single lipid raft would provide n docking sites for M1A on the cytoplasmic face. By providing a platform for the formation of a small M1A ensemble, lipid rafts may eliminate the low duty ratio limitation (provided that n
10) and allow the multiple SI molecules in a given raft to maintain a continuous link to the underlying cytoskeleton. Alternatively, because the kinetics of M1A may be strain dependent (Jontes et al., 1997), its duty ratio may range from
0.1 (in the unloaded case) to much higher values in the presence of load (e.g., a stable interaction with SI). Thus, a single M1A molecule, with appropriate loading, might be capable of retaining SI in the absence of a larger motor cluster. Moreover, although FRAP studies have demonstrated that most of the M1A in the BB exchanges rapidly, these data also indicate that a smaller population does not turnover on the time scale of the observations (Tyska and Mooseker, 2002). We speculate that this "immobile" fraction may represent lipid raft-associated M1A engaged in microvillar retention. In support of this hypothesis, our biochemical data indicate that 1020% of the total microvillar population of M1A associates with SI-containing lipid rafts, a fraction that is similar in magnitude to the immobile fraction provided by FRAP studies (923%; see Table I; Tyska and Mooseker, 2002).
M1A was discovered as the first vertebrate class I myosin over a decade ago (Cheney and Mooseker, 1992); the investigation we present here is the first to define a specific function for this motor. In combination with other available evidence, our data suggest that M1A is involved in the retention of SI in the BB, most likely through an interaction with SI-lipid raft complexes. These findings also complement a recent proteomic study that highlighted myosin-1G as a component of DRMs from neutrophils (Nebl et al., 2002). Given that myosins-I are ubiquitously expressed, we predict that future studies will reveal that these motors are lipid raft components in a variety of cell types. Because lipid rafts are thought to function as dynamic signaling platforms with the ability to recruit or exclude specific components based on their biochemical environment and/or extracellular stimuli, future studies will focus on identifying factors that might regulate the interaction between M1A and SI-containing lipid rafts in the MV.
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Materials and methods |
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Cloning of GFP-M1A-tail and SINT-YFP fusion constructs
The GFP-M1A-tail and SINT-YFP fusion constructs were assembled using standard molecular biological techniques. To fuse GFP to the COOH-terminal tail of M1A (aa 7721043, Tail), PCR with the full-length human M1A cDNA (Skowron et al., 1998) as template was used to create a fragment with 5' XhoI and 3' XmaI sites that allowed for ligation into the pEGFP-C1 polylinker. Next, PCR was used to create a GFP-M1A-tail fragment with 5' SalI and 3' ClaI sites that allowed for ligation into the polylinker of the retroviral vector pLNCX2 (CLONTECH Laboratories, Inc.). To create the dominant negative fragment SINT-YFP, an oligo with 5' SacI and 3' XmaI sites encoding the sequence MARKKFSGLE-GG (the 10 NH2-terminal cytoplasmic residues of human SI and a double-glycine linker) was annealed with its reverse complement, cut and ligated into the pEYFP-N1 polylinker.
Transfections and retroviral infections
Standard transfection protocols recommended for Lipofectamine 2000 (GIBCO BRL) were used to generate BBE stable lines expressing SINT-YFP, whereas stable lines expressing GFP-M1A-tail were generated with a retroviral system. In both cases stable integration was necessary because these cells are grown at confluency for several weeks to achieve a fully differentiated state. To generate recombinant retrovirus for the purpose of infecting BBE cells, PT67 packaging cells (CLONTECH Laboratories, Inc.) at 7080% confluency were transfected with pLNCX2-GFP-M1A-tail using Lipofectamine 2000 (GIBCO BRL). 48 h after transfection, cells were replated and grown in maintenance medium supplemented with 1 mg/ml G418 (GIBCO BRL) for 2 wk. Media supernatants from virus-producing PT67 cultures were filtered with a low protein-binding (0.04 µm) syringe filter, supplemented with 5 µg/ml hexadimethrine bromide (Sigma-Aldrich), and used to infect preconfluent BBE cells. Stable BBE transformants were selected with 1 mg/ml G418. For these studies, GFP-M1A-tail expressing cells were compared with neighboring cells that demonstrated very low or no expression. This approach was useful as it provided an internal control for variability induced by G418 selection or differences in differentiation state. None of the phenotypes described in this work were observed in cells transfected with EGFP or EYFP alone.
Immunofluorescence staining
Fully differentiated BBE cells grown on filters (Corning-Costar) were fixed using the pH-shift fixation protocol originally described by Bacallao and Stelzer (1989). In brief, cells were rinsed with prewarmed buffer 6.5 (80 mM K-Pipes, 5 mM EGTA, 2 mM MgCl2, pH 6.5) and then fixed with 3% PFA in buffer 6.5 for 5 min at RT. After aspirating the initial fix, cells were then fixed with 3% PFA in buffer 11 (100 mM NaB4O7, pH 11.0) for 10 min at RT. Cells were then rinsed twice in PBS, pH 8.0, and incubated with 1 mg/ml NaBH4 in PBS for 15 min. After a thorough rinsing in PBS, cells were permeabilized in 0.1% Triton X-100 for 5 min and blocked in 10% BSA for 20 min. Incubation in primary antibodies for 45 min at 37°C was followed by several washes in PBS, and then incubation in secondary antibodies with the appropriate phalloidin conjugate (1:200) for 20 min at RT. After a final series of washes, filters were cut out of plastic supports, mounted with Citifluor antifade reagent (Ted Pella, Inc.), and stored at 4°C until imaging.
Antibodies for immunofluorescence
The following primary antibodies and corresponding dilutions were used: antihuman M1A-tail pAb (Skowron et al., 1998), 20 µg/ml; antihuman APN mAb, 1:200 (Biosource International); CX-1, an mAb raised against chicken M1A that recognizes the head domain of multiple myosins (Carboni et al., 1988), 1:100 dilution of ascites fluid; anti-AP polyclonal, 1:500 (Sigma-Aldrich); antihuman SI mAb (a gift from A. Quaroni, Cornell University, Ithaca, NY), 1:500. Secondary antibodies or phalloidin conjugated to either Alexa-488 or Alexa-568 (Molecular Probes) were used at 1:500.
Light microscopy
All confocal images were acquired on a laser scanning confocal microscope (model MRC-1024; Bio-Rad Laboratories) using 40x/1.0 or 63x/1.4 Plan Apochromat objectives (Carl Zeiss MicroImaging, Inc.). Still images of fixed cells in the plane parallel to the coverslip surface (x-y) were acquired by Kalman (Bio-Rad Laboratories) averaging three to five scans with the scan rate to normal. All en face images were taken at the level of the BB. Vertical sections (z) were scanned with resolution set to high and scan rate set to normal. Two-color images were acquired with sequential scanning. Images were LUT stretched, pseudo-colored, and overlaid using Metamorph (v. 5.0; Universal Imaging Corp.), and then assembled into figures using Photoshop (v. 5.5; Adobe) or PowerPoint (v. X; Microsoft). For the paired-intensity analysis used to assess the effects of GFP-M1A-tail and SINT-YFP expression on SI localization, Metamorph was used to LUT stretch images so that the 8-bit grayscale values in both channels ranged from 0 to 256 with minimal saturation. The average intensities of both signals for a given cell were recorded and then plotted as coordinates (v. 7.0; Sigmaplot) to determine the degree of correlation.
SDS-PAGE and immunoblotting
Protein fractions were analyzed with SDS-PAGE using 520% gradient gels. For immunoblotting, gels were transferred to nitrocellulose (85 V, 3.5 h) at 4°C. Stock solutions of affinity-purified primary antibodies were diluted 1:1,000 before use and immunogens were visualized using the ECL method according to the manufacturer's instructions (Amersham Biosciences). Primaries used in this study that were not mentioned above include anti-GLUT5 (Calbiochem), anti-CaM mAb (Upstate Biotechnologies), antimyosin-IE (Skowron et al., 1998), antimyosin-VI (Hasson and Mooseker, 1994), antimyosin-V (Suter et al., 2000), antimyosin-IXb (Wirth et al., 1996), anti-actin mAb (Sigma-Aldrich), and anti-villin mAb (AMAC).
Preparation of DRMs from BBE cells
Confluent BBE cells were rinsed three times with TBS at 37°C, scraped from the flask, and pelleted (1500 g, 10 min). Subsequent steps were performed at 4°C. Cells were resuspended in nine pellet volumes of buffer 2xA' (2 mM EGTA, 150 mM KCl, 5 mM MgCl2, 1 mM DTT, 1 mM Pefabloc, 40 mM imidazole, pH 7.2) supplemented with 1 mM ATP and 1% Triton X-100, and then homogenized in a stainless steel dounce (Fig. 4 A, Hmg). The postnuclear supernatant (2 ml; Fig. 4 A, PNS) from this homogenate was underlayed with 1 ml 48% OptiPrepTM (Sigma-Aldrich) and spun at 100,000 g for 60 min at 4°C in a Beckman TLA 110 rotor. Crude DRMs were collected from the OptiPrepTM cushion (Fig. 4 A; Interface) and resuspended to 1 ml of 30% OptiPrepTM with 2xA' and 60% OptiprepTM. Density gradients were generated by spinning the resuspended DRMs at 350,000 g for 2 h at 4°C in a Beckman TLA 120.2 rotor. Gradient fractions (11 x 90 µl) were taken and subject to cholesterol determination with the Amplex Red assay (Molecular Probes), density determination with a refractometer (model 334610; Bausch & Lomb), SDS-PAGE, and immunoblot analysis as described above.
Preparation of DRMs from rat MV
BBs were purified from rat small intestine as described elsewhere (Keller and Mooseker, 1982). Microvillar purification and subsequent DRM isolation were performed as described by Mooseker et al. (1989). All steps were performed at 4°C. In brief, microvillar pellets (Fig. 6, D and E, MV) were resuspended in buffer A (1 mM EGTA, 75 mM KCl, 5 mM MgSO4, 1 mM DTT, 1 mM Pefabloc, 10 mM imidazole, pH 7.2) supplemented with 1% Triton X-100 and allowed to incubate on ice for 5 min. Demembranated MV were collected by sedimentation at 40,000 g for 15 min and washed several times in 20 vol buffer A with 1% Triton X-100. After the final wash, M1A and DRMs were released from microvillar cores by resuspension in 5 vol of buffer A supplemented with 1% Triton X-100 and 2 mM ATP (Fig. 6, D and E, ATP release S), followed by sedimentation at 40,000 g for 15 min (Fig. 6, D and E, ATP release P). For density gradient analysis, the ATP release S fraction was adjusted to 30% OptiPrepTM with 2xA supplemented with 1% Triton X-100 and 2 mM ATP, and 60% OptiPrepTM, and then spun at 350,000 g for 2 h at 4°C in a Beckman TLA 120.2 rotor. Gradient fractions (11 x 90 µl) were taken and subject to density determination with a refractometer (model 334610; Bausch & Lomb) and SDS-PAGE/immunoblot analysis as described above. Protein concentrations were determined with the BCA assay (Pierce Chemical Co.). To generate a highly purified DRM fraction for EM (Fig. 7), DRMs were sedimented from the ATP release S fraction at 100,000 g, then resuspended and subject to density gradient centrifugation as described above. The lowest density fraction with SI (
1.12 g/ml) was taken for negative stain and immunoblotting.
Affinity purification of DRMs
For the affinity purification of SI-containing DRMs, CL-4B agarose resin with immobilized streptavidin (Sigma-Aldrich) was incubated overnight at 4°C in the presence (ConA beads) or absence (control beads) of 0.5 mg/ml biotin-ConA (Sigma-Aldrich). The next day, beads were washed five times with 2xA' supplemented with 1 mM ATP and 1% Triton X-100 and added to DRMs in 2xA' at a final concentration of 10% (vol/vol). After incubating for 4 h at 4°C, beads were washed five times with 2xA' supplemented with 1 mM ATP and 1% Triton X-100, resuspended in volumes equal to the supernatant, and processed for SDS-PAGE/immunoblotting. To disrupt purified DRMs, aliquots of the ConA agarose (with DRMs bound) were incubated at 37°C for 30 min or at RT with 2% MßCD for 30 min. Bead pellets were then resuspended in volumes equal to the supernatant and processed for SDS-PAGE/immunoblotting.
Immunopurification of DRMs
SI-containing DRMs from rat MV were immunopurified with an antirat SI pAb (a gift from A. Quaroni). The ATP release S fraction (Fig. 6, D and E) was first precleared by incubating with 10% protein-A Sepharose (PAS; Amersham Biosciences), for 1 h at 4°C. Next, 10% PAS and equal quantities (5 µg) of either anti-SI or nonimmune rabbit IgG (Jackson ImmunoResearch Laboratories) were added to equal volumes of supernatant and samples were incubated overnight at 4°C. The next day, PAS pellets were washed five times with 2xA' supplemented with 1 mM ATP and 1% Triton X-100, followed by processing for SDS-PAGE and immunoblotting as described above.
Chemical cross-linking
DRMs released from rat MV as described above were chemically cross-linked by the addition of EDC (Pierce Chemical Co.) to a final concentration of 50 mM and incubation on ice for 10 min, and then RT for an additional 10 min. The reaction was quenched by adding 1 vol of sample buffer supplemented with 500 mM ß-mercapto-ethanol. Cross-linked products were separated with SDS-PAGE using NuPage Novex 38% Tris-acetate gradient gels (Invitrogen) and processed for immunoblotting as described above.
Transmission EM
Rat MV and DRMs were negatively stained with 1% uranyl acetate on nitrocellulose, carbon-coated grids that were glow-discharged immediately before use.
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Acknowledgments |
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This work was supported by National Institutes of Health grants DK-25387 (to M.S. Mooseker), DK-55389 (to Jon Morrow), and National Research Service Award DK-10113 (to M.J. Tyska).
Note added in proof. Portions of the data reported here have been published previously in abstract form (Tyska, M.J., and M.S. Mooseker. 2002. Mol. Biol. Cell. 13:179a; and Tyska, M.J., and M.S. Mooseker. 2003. Mol. Biol. Cell. 14:178a).
Submitted: 7 October 2003
Accepted: 2 April 2004
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