Article |
Address correspondence to Christian Fuhrer, Brain Research Institute, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland. Tel.: 41-1-635-33-10. Fax: 41-1-635-33-03. E-mail: chfuhrer{at}hifo.unizh.ch
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Abstract |
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Key Words: laminin; tyrosine phosphorylation; neuromuscular junction; clustering; acetylcholine receptors
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Introduction |
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Nerve-independent and neural agrininduced clustering of AChRs both require the receptor tyrosine kinase MuSK (Glass et al., 1996; Lin et al., 2001; Yang et al., 2001). Agrin activates MuSK, which initiates a signaling cascade that requires the AChR-associated protein rapsyn to cause clustering of AChRs (Gautam et al., 1995; for review see Sanes and Lichtman, 2001; Huh and Fuhrer, 2002). Via this pathway, agrin also induces clustering of many other postsynaptic proteins, leading to coextensive aggregates of these proteins with AChRs (Wallace, 1989; Meier et al., 1997; Marangi et al., 2001). Within the agrin signaling pathway, AChRs play an active role in the clustering of several postsynaptic proteins, including rapsyn (Marangi et al., 2001). Furthermore, agrin causes rapid activation of Src-related kinases and tyrosine phosphorylation of AChR ß and subunits (Mittaud et al., 2001); phosphorylation of ß is required for efficient AChR clustering (Borges and Ferns, 2001). Finally, after AChR clusters are formed, tyrosine phosphorylation and Src and Fyn kinases are necessary for cluster stabilization (Ferns et al., 1996; Smith et al., 2001).
AChR clustering in myotubes is also induced by laminin-1 and laminin-2/4 (merosin) (Sugiyama et al., 1997; Montanaro et al., 1998; Burkin et al., 2000). The distribution of laminins in the muscle basal lamina is under tight developmental control (Patton et al., 1997). Laminin-1 is expressed early in development and, although hardly detectable at the NMJ itself, at the time of neuromuscular synaptogenesis (E15) before it disappears from muscles later on. Laminin-2 is expressed throughout development, whereas expression of laminin-4 starts at the synapse during synaptogenesis, resulting in a strong expression of merosin (laminin-2/4) at the earliest synapses (E15). Laminin-4 expression persists mainly synaptically at later stages (Patton et al., 1997). Thus, all laminins capable of inducing AChR clusters are expressed during NMJ formation, and NMJs of all developmental stages always contain at least one of these laminin isoforms. These observations are consistent with an important role of laminin-1 and laminin-2/4 in AChR clustering during NMJ formation. Synaptic laminins also direct other aspects of NMJ development. In the absence of the ß2 chain, NMJs display less postjunctional folds, poor presynaptic differentiation, and Schwann cell invasion into the synaptic cleft (Noakes et al., 1995), whereas without the 4 chain, active zones are not aligned with junctional folds (Patton et al., 2001).
The actual mechanism of AChR clustering induced by laminin-1 and laminin-2/4 remains poorly characterized, and it is likewise unknown whether these laminins cause clustering of many other proteins in addition to the AChR. However, laminin-1 and laminin-2/4 appear to activate the same pathway (Burkin et al., 2000), which is known to differ from the agrin pathway in several ways. First, some laminin-induced AChR clustering can occur independently of MuSK in MuSK-/- myotubes, although at lower levels (at least 20 times) than in wild-type cells (Sugiyama et al., 1997). Second, short laminin treatments (1545 min) do not cause phosphorylation of the AChR ß subunit (Sugiyama et al., 1997; Montanaro et al., 1998). These observations suggest that laminin may act independently of tyrosine kinases. Finally, laminin-induced AChR clustering shows slower kinetics and leads to larger AChR clusters with higher AChR densities than in the case of agrin (Sugiyama et al., 1997; Montanaro et al., 1998).
Nevertheless, the agrin and laminin pathways share some common principles. Both agrin and laminin are known to require dystroglycan to induce AChR clusters of normal morphology and stability in myotubes (Jacobson et al., 1998, 2001; Montanaro et al., 1998; Grady et al., 2000). Agrin and laminin act synergistically in AChR clustering and laminin-pretreated myotubes require much less agrin for subsequent clustering, suggesting that laminin primes myotubes to be more sensitive towards agrin (Sugiyama et al., 1997; Burkin et al., 2000). Furthermore, integrins such as 7ß1 are implied as receptors in both laminin- and agrin-induced AChR clustering, because in myotubes antibodies against ß1 or
7 integrin modulate both clustering processes (Martin and Sanes, 1997; Burkin et al., 1998).
7ß1 integrin is more frequently found at AChR clusters induced by laminin than by agrin, but the integrin is also prominent outside of such clusters (Burkin et al., 1998). These data suggest that agrin and laminin act together to achieve maximal AChR clustering. In support of this idea, agrin binds directly to laminin-1, -2, and particularly laminin-4 (Denzer et al., 1997), which may contribute to the synaptic concentration of agrin.
An intrinsic property of laminins is to build extracellular matrices by forming an organized network with other proteins, which stabilizes cells by interaction with cytoskeleton-linked surface receptors (Henry and Campbell, 1999). Furthermore, laminins 14 polymerize while bound to muscle receptors like integrins and dystroglycan, leading to reorganization of these receptors into a network-like structure or small clusters that colocalize with laminin (Cohen et al., 1997; Colognato et al., 1999). Thus, laminin-induced AChR clustering could, in principle, occur by extracellular protein interactions that redistribute dystroglycan and other members of the dystrophin/utrophin glycoprotein complex (D/UGC), followed by redistribution of AChRs, which are known to interact with the D/UGC (Fuhrer et al., 1999). Together, these various lines of evidence imply that laminin-induced AChR clustering may not require intracellular signaling, in particular tyrosine kinases.
Therefore, to unravel the mechanism by which laminin causes AChR clustering, we have analyzed whether laminin redistributes postsynaptic proteins and requires an intracellular signaling cascade similar to that of agrin. Because laminin-1 is best characterized in terms of inducing AChR clustering, we concentrated on this form of laminin. We find that laminin-1 differentially redistributes postsynaptic proteins into a network or AChR-containing clusters, in contrast to agrin, which is known to cause co-clustering of all these proteins with AChRs. Furthermore, laminin-1 initiates phosphotyrosine signaling that, similar to agrin, requires rapsyn and tyrosine kinases for formation of AChR clusters, whereas Src and Fyn are necessary for stabilization of clusters.
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Results |
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Interestingly, the localization of AChR clusters induced by laminin-1 showed no obvious correlation with the distribution of laminin-1 itself (Fig. 1 B; see Fig. 3). Therefore, we systematically analyzed how other proteins were distributed after laminin treatment. By assessing the distribution of - and ß-dystroglycan, syntrophin isoforms, rapsyn, tyrosine-phosphorylated proteins, MuSK, utrophin, and
-dystrobrevin-1, we observed three different classes of proteins. The first class, including
- and ß-dystroglycan and syntrophins, was rearranged into network-like patches by laminin-1 and showed no obvious colocalization with AChR clusters (Fig. 2 A). These patches resembled the distribution of laminin-1 itself (Fig. 1 B). Indeed, pairwise double staining revealed partial colocalization of laminin-1 with
-dystroglycan, and, to a lesser degree, with ß-dystroglycan and syntrophin (unpublished data).
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Finally, the third class of proteins, comprising rapsyn- and tyrosine-phosphorylated proteins, formed laminin-induced clusters as efficiently as AChRs and strictly colocalized with AChRs in clusters (Fig. 3 A). The intensity of laminin-induced phosphotyrosine aggregates was high and appeared brighter than in the case of agrin (Fig. 3 A; unpublished data). For quantitation, we analyzed three different AChR cluster populations (510 µm, 1030 µm, and clusters >30 µm in length; Fig. 3 B, left) and their corresponding phosphotyrosine aggregates (unpublished data). Laminin-1 increased the number of AChR and phosphotyrosine clusters of all sizes, with the total increase of all AChR clusters being 5.8 ± 2.6-fold (mean ± SD; 12 experiments; Fig. 3 B) and of phosphotyrosine clusters being 5.8 ± 1.6-fold (mean ± SD; seven experiments; unpublished data). Furthermore, all AChR clusters colocalized efficiently with phosphotyrosine-containing proteins showing an overall colocalization of 88% (Fig. 3 B, right). Triple staining for laminin (blue), AChR (red), and phosphotyrosine (green), and subsequent merging of digitized pictures confirmed the high colocalization between AChR and phosphotyrosine clusters (Fig. 3 C, yellow clusters). In contrast, when pictures of surface-bound laminin were merged to AChRs or phosphotyrosine, almost no overlap could be detected.
Together, these data show that postsynaptic proteins are differentially distributed by laminin-1 (Table I). Most strikingly, phosphotyrosine and rapsyn cluster very efficiently in response to laminin, and strictly colocalize with AChRs. This reveals a difference to agrin, which induces significant coclustering with AChRs of all the postsynaptic proteins examined here, including dystroglycans, syntrophins, and MuSK (Table I; Marangi et al., 2001).
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Discussion |
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Laminin-1 redistributes dystroglycans and syntrophin
Laminin-1, as well as laminin-2/4, can bind to myotube surface receptors and form cell-associated polygonal networks (Colognato et al., 1999; Montanaro et al., 1999). Furthermore, -dystroglycan is a binding partner for laminins (Henry and Campbell, 1999). Our observed laminin network differs from that described by Colognato et al. (1999), in that although we occasionally see exogenous laminin-1 in a regular polygonal arrangement (PAM and STW, unpublished data), most of our laminin-1 appears as bright and bulky deposits forming a rather coarse network. This most likely originates from the higher concentration of our laminin used, which is required in order to cause AChR clustering.
In our experiments, - and ß-dystroglycan and syntrophins codistribute with exogenously applied laminin-1. This codistribution of dystroglycans is consistent with the known tight binding of laminin directly to
-dystroglycan (Henry and Campbell, 1999). Our antibody against syntrophin detects all muscle syntrophin isoforms, including extrasynaptic ones. Others have found that dystrophin codistributes with laminin, at least in a polygonal network when low concentrations of laminin-1 are added to myotubes (Colognato et al., 1999). Together, these data indicate that components of the dystrophinglycoprotein complex are redistributed by laminin, and laminin-1 appears able to organize this complex from the extracellular matrix to the underlying cytoskeleton. Laminin-1 may thereby contribute to muscle stability extrasynaptically.
Laminin-1 clusters postsynaptic proteins
The fact that laminin-1 redistributes and colocalizes with some muscle proteins (as described above; Table I) raised the possibility that laminin may drive clustering of AChRs merely by extracellular protein rearrangements, because AChRs indirectly interact with dystroglycan (Fuhrer et al., 1999). However, we found that laminin-induced AChR clusters hardly colocalize with exogenously applied laminin itself, nor with dystroglycans or syntrophins. Rather, laminin-1 caused clustering of the synaptic proteins utrophin and -dystrobrevin-1, and these clusters colocalized strongly with AChRs. In the case of MuSK, laminin-1 induced a marginal clustering. Furthermore, laminin caused aggregation of rapsyn and phosphotyrosine, which colocalized precisely with AChR clusters but showed very little overlap with the laminin network distribution (Table I). The regular hexagonal network that is formed by applying low laminin concentrations to myotubes can be disassembled by genistein, showing that its formation requires tyrosine phosphorylation (Colognato et al., 1999). Interestingly, we found that staurosporine and herbimycin did not affect the distribution of laminin-1 yet abolished AChR clustering. Similarly, genistein did not affect the distribution of laminin-1 in our conditions (STW, PAM, and CF, unpublished data). These observations show that laminin-1 does not act in AChR clustering merely by protein redistribution based on extracellular protein interactions. If involved at all, the laminin network clearly is not sufficient for clustering. Rather, clustering involves intracellular signaling mechanisms.
Laminin-induced AChR clustering requires rapsyn and tyrosine phosphorylation
Based on the coclustering of rapsyn with AChRs induced by laminin-1, we investigated the laminin signaling pathway by examining the role of rapsyn. Use of rapsyn -/- myotubes showed that indeed rapsyn is required for clustering of AChR and phosphotyrosine, very similar to its role in agrin signaling. Rapsyn acts in agrin signaling downstream of MuSK (Apel et al., 1997). As laminin-induced AChR clustering can occur in the absence of MuSK (Sugiyama et al., 1997), the agrin and laminin pathways thus converge at a step downstream of MuSK involving rapsyn. This role of rapsyn reconfirms that laminin requires intracellular signaling mechanisms for AChR clustering.
We investigated this signaling mechanism in more detail by analyzing the role of tyrosine phosphorylation. Laminin induced phosphorylation of AChR subunits, mostly on the ß subunit. These results extend earlier findings that short (1545 min) laminin treatments do not induce phosphorylation of the AChR ß subunit and of MuSK (Sugiyama et al., 1997; Montanaro et al., 1998). In our hands, AChR ß subunit phosphorylation paralleled clustering, as it was detectable after 9 h and strongest after 24 h. In contrast, in the case of agrin, AChR ß phosphorylation precedes cluster formation and peaks after about 1 h (Ferns et al., 1996). Thus, the mechanisms that trigger ß phosphorylation are different between agrin and laminin. Furthermore, although in our biochemical experiments ß phosphorylation is relatively weak after laminin treatment, phosphotyrosine clustering is very intense, implying that other proteins are tyrosine-phosphorylated and co-clustered with AChRs.
However, most importantly, the same kinase inhibitors that block AChR phosphorylation and clustering by agrin, herbimycin and staurosporine, also inhibit laminin-induced aggregation of AChRs and block phosphorylation of AChR ß subunits. These results raise the possibility that phosphorylation of the AChR itself, on its ß subunit, may be important for clustering, in analogy to the role of ß phosphorylation in receptor clustering induced by agrin (Borges and Ferns, 2001). Several control experiments indicated that the effects of herbimycin and staurosporine during their 89 h incubation are specific and not due to general toxicity of these chemicals. First, the inhibitors did not affect the general morphology of C2 (Fig. 6 A). Second, when treated with inhibitor for 8 h, followed by extensive washing and incubation with laminin or agrin in the absence of inhibitor, C2 cells formed normal AChR clusters (STW and CF, unpublished data). Finally, when adding agrin and inhibitors simultaneously for 8 h (i.e., leaving out the normal 1-h preincubation with inhibitor), C2 formed normal clusters (STW and CF, unpublished data).
These data reveal that laminin activates one or several tyrosine kinases, and that this activity is required for laminin-, as well as for agrin-induced AChR clustering. This kinase(s) is downstream of MuSK, because staurosporine does not affect MuSK activation (Fuhrer et al., 1997), and may be a member of the Src family, because such kinases are activated in response to agrin (Mittaud et al., 2001). Src family kinases, together with focal adhesion kinase FAK, are activated in integrin signaling, which leads to tyrosine phosphorylation and rearrangement of cytoskeletal proteins such as paxillin (Thomas and Brugge, 1997; Thomas et al., 1998). Paxillin, talin, and -fodrin are localized postsynaptically at the NMJ and are substrates for Src family kinases (Hall and Sanes, 1993; Thomas and Brugge, 1997). Thus, given the role of
7 and ß1 integrins in both laminin and agrin-induced AChR clustering, signaling mediators and cytoskeletal substrates downstream of integrins, in particular tyrosine kinases, are good candidates to be involved as a common element in laminin as well as agrin signaling.
Src and Fyn are required for stabilization of laminin-induced AChR clusters
Similar to agrin, Src and Fyn are not necessary for laminin-induced AChR clustering. However, the Src-related kinase Yes is upregulated in src -/-;fyn -/- myotubes, and might thus compensate (Smith et al., 2001). In contrast, these mutant cells show that Src and Fyn are required for maintaining AChR clusters after laminin-withdrawal. Furthermore, tyrosine phosphorylation as such is required for cluster stabilization, as shown by herbimycin and staurosporine. These findings reveal strong parallels to agrin, where tyrosine phosphorylation, Src, and Fyn are all required for stabilizing AChR clusters (Ferns et al., 1996; Smith et al., 2001). Src and Fyn may act in stabilization of laminin- or agrin-induced AChR clusters through their adaptor activities, by mediating protein interactions using their SH2 or SH3 domains (Smith et al., 2001). Such kinase activityindependent roles are known from focal adhesion sites, where kinase-inactive Src can recruit FAK (Kaplan et al., 1995; Thomas and Brugge, 1997). Alternatively and/or in parallel, Src and Fyn may act by phosphorylating other proteins. A good candidate is dystrobrevin, which is highly phosphorylated in Torpedo electric organ (Wagner et al., 1993). Interestingly, laminin-1 clusters -dystrobrevin-1 in a similar fashion as agrin does, and agrin-induced aggregates of AChRs are unstable in the absence of
-dystrobrevin (Grady et al., 2000). Thus, these data raise the possibility that Src and Fyn act in stabilization of AChR clusters (induced by agrin or laminin) by linking the receptor to components of the D/UGC such as dystrobrevin.
The laminin signaling pathway activates the agrin signaling pathway
Previous studies suggested that laminin-1 acts through a different pathway than agrin (Sugiyama et al., 1997; Montanaro et al., 1998), largely based on the differential role of MuSK and rapid AChR ß phosphorylation. However, our results demonstrate that laminin-1 activates an intracellular signaling pathway that strongly overlaps with that of agrin at an early step, downstream of MuSK, at the level of tyrosine kinases and rapsyn. Thus, rapsyn and tyrosine phosphorylation (possibly also of AChR ß) are required for both agrin- and laminin-induced AChR clustering. Furthermore, laminin and agrin induce comparable coclustering of utrophin and -dystrobrevin-1 with AChRs. Nonetheless, other findings argue against a full convergence of both pathways, because agrin and laminin have additive effects when added together to myotubes, even when agrin is applied at a saturating concentration (Sugiyama et al., 1997; Burkin et al., 2000). Thus, neither molecule alone seems able to fully activate the signal transduction pathway leading to clustering, and only combined application allows maximal aggregation. Therefore, we think that the laminin and agrin pathways share rapsyn and one or several kinases but also each have unique signaling steps.
This cooperation, particularly on the level of tyrosine kinases, may be important for postsynaptic organization in vivo. Myotubes are known to contain ample tyrosine phosphatase activity that continuously dephosphorylates proteins such as the AChR (Wallace, 1995). Upon blockage by pervanadate (Wallace, 1995), or treatment with agrin (Borges and Ferns, 2001), AChRs become stably phosphorylated and linked to the cytoskeleton, showing that agrin, by activating tyrosine kinases, overcomes the phosphatase activity, thereby driving clustering. By activating the same downstream kinases as agrin, laminin may create an intracellular signaling environment in the muscle that primes myofibers to be more receptive towards a clustering signal like agrin. Consistent with this, laminin-pretreated myotubes require 20-fold less agrin to trigger subsequent AChR clustering (Burkin et al., 2000). Because activated kinases can diffuse intracellularly, such a priming mechanism would operate along considerable distances within muscle fibers and not be restricted to the location of laminin, in agreement with our observation that laminin deposits hardly colocalize with AChR clusters. Based on expression patterns (Patton et al., 1997), laminin-1 may cause widespread signaling throughout the muscle and render muscle fibers (which grow from the middle extending longitudinally) receptive for a clustering signal early in development and around the period of synapse formation, as laminin-1 is lost later. Similarly, laminin-2/4 (which induces AChR clusters indistinguishably from laminin-1 in cultured myotubes; Burkin et al., 2000) may prime the muscle, particularly local synaptic areas, to be receptive around synaptogenesis and later. Neural agrin may enhance such local laminin-2/4-signaling, and the fine tuning of this signaling with the more widespread effects of laminin-1 may be an underlying mechanism for AChR clustering in the middle of myofibers.
It is possible that at certain developmental stages, depending on the level of laminin expression, such a laminin priming mechanism may actually lead to AChR clusters independently of agrin. This may be the case at the earliest stages of NMJ formation, where muscle prepatterning leads to AChR clustering independently of neural agrin and even motorneurons (Yang et al., 2000, 2001; Lin et al., 2001). At this stage, laminin-induced AChR clustering may involve MuSK, given the absence of early clusters in MuSK -/- mice (Lin et al., 2001; Yang et al., 2001), and may occur by phosphorylation and activation of MuSK not through agrin-like ligands but through the downstream kinases activated by laminin. In agreement with this, MuSK can be phosphorylated by other kinases such as Src or Fyn in myotubes (Mohamed et al., 2001), and laminin-induced AChR clustering occurs to a reduced degree in MuSK -/- myotubes in vitro (Sugiyama et al., 1997). Furthermore, upon ectopic injection into myofibers in vivo, MuSK causes AChR clustering independent of the MuSK ectodomain (which would interact with agrin-like ligands) but dependent on its kinase domain (Sander et al., 2001).
However, as development proceeds, laminin does not seem able to cause efficient nerve-bound clustering of AChR in the absence of agrin, because no such clusters are seen at birth in agrin -/- mice (Gautam et al., 1996). However, the remaining few clusters seen in these animals at birth, could raise from laminin. It remains to be investigated how cooperation between agrin and laminin is regulated in vivo and how their signaling pathways mediate AChR clustering throughout development.
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Materials and methods |
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Agrin, laminin, and tyrosine kinase inhibitors
Laminin-1, purified from basement membranes of Engelbreth-Holm-Swarm Mouse sarcoma, was purchased from Sigma-Aldrich (L-2020; Fluka Chemie AG, Buchs; Sugiyama et al., 1997). To induce clustering, 100 nM laminin-1 was added for 16 h to C2, src -/-;fyn -/-, and rapsyn -/- cells on day 2, day 34, and day 23 in fusion medium, respectively. As control, agrin-induced clustering and phosphorylation was examined in parallel. COOH-terminal constructs of neurally derived agrin (C-Ag12,4,8) were expressed and quantitated as described earlier (Fuhrer et al., 1997). To study the effects of kinase inhibitors on laminin-induced clustering of AChRs and phosphotyrosine, staurosporine (S-5921; Sigma-Aldrich) and herbimycin A (Life Technologies) were used as described below.
Precipitation assays and immunoblot analysis
To examine laminin-induced tyrosine phosphorylation of AChRs in C2 myotubes, cells were treated with 80 nM laminin for 324 h, and lysates were subjected to AChR precipitation using biotin--btx (Molecular Probes) (Mittaud et al., 2001). As controls, cells were treated for 1 h with 0.25 nM agrin, or an excess (10 µM) of free
-btx was added after a 24-h laminin treatment before AChR precipitation. To inhibit laminin-induced AChR phosphorylation, C2 cells were pretreated with 1 µM herbimycin or 10 nM staurosporine for 1 h, followed by 78 h treatment with 120 nM laminin in the presence of the inhibitors. After biotin-
-btx precipitation, tyrosine-phosphorylated proteins were detected by immunoblotting using a mixture of 4G10 (Upstate Biotech) and PY-20 antibodies (Transduction Labs). To visualize precipitated AChRs, blots were stripped and reprobed for the AChR ß subunit with mAb124 antibodies or for the AChR
/
subunits with mAb88B, provided by Dr. S.C. Froehner (University of Washington, Seattle, WA). Quantitation was performed by densitometric scanning as described previously (Marangi et al., 2001).
Immunocytochemical staining procedures
AChRs were visualized by incubating cells with 100 nM tetramethylrhodamine-conjugated -btx (Molecular Probes) in fusion medium for 1 h at 37°C before fixation and double staining for other postsynaptic markers. Staining procedures for
-dystroglycan, ß-dystroglycan, syntrophins (
-, ß1- and ß2-isoforms), rapsyn, MuSK,
-dystrobrevin-1, utrophin, and tyrosine-phosphorylated proteins were all done as described previously, using fluorescein-coupled secondary antisera (Marangi et al., 2001). To detect exogenously added laminin-1 in double or triple stains, purified laminin antibodies mAb1905 (Chemicon) were added for 1 h at room temperature together with
-btx before fixation and addition of other primary antibodies. Cells were then treated with secondary Alexa Fluor 350conjugated goat antirat antibodies (Molecular Probes) to detect mAb1905 and with fluorescein-coupled secondary or tertiary antibodies, to detect the other primary antibodies. Although mAb1905 is able to detect endogenous laminin-1 in C2 myotubes, this signal is neglectable in comparison to the much stronger intensity seen in laminin-1treated cells stained with mAb1905 (Fig. 1 B). Finally, in all cases, cells were mounted in a medium containing glycerol and p-phenylenediamine (Sigma-Aldrich), to reduce fluorescence fading.
Fluorescence microscopy and quantitation of clusters
Stained myotubes were examined with a fluorescence microscope and signals on pictures were counted as clusters as previously described (Marangi et al., 2001). Such signals had to be at least 10 µm in length to score as a cluster. However, in Figs. 3 and 6, we also counted clusters of 510 µm in length. For laminin-induced clustering of MuSK, -dystrobrevin, and utrophin, at least 10 pictures per treatment were counted in each experiment and the number of clusters averaged. Data from four such experiments were used to calculate mean ± SD. Clusters of AChRs and phosphotyrosine were quantitated in one of several ways, which all gave similar results. In Figs. 3 and 6, pictures taken at 200x magnification (from at least three experiments) were evaluated as described above for MuSK,
-dystrobrevin, and utrophin. Similarly, in Figs. 9 and 10, pictures taken at 400x magnification (from at least five experiments) were evaluated to calculate mean ± SEM. Finally, in Fig. 8, a total of 20 pictures (400x magnification) per treatment were counted to yield mean ± SD of AChR clusters.
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Footnotes |
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Acknowledgments |
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This work was supported by the Dr. Eric Slack-Gyr Foundation, the National Center of Competence in Research "Neural Plasticity and Repair," and by grants from the Swiss National Science Foundation and the Swiss Foundation for Research on Muscle Diseases (to C. Fuhrer).
Submitted: 22 February 2002
Revised: 22 April 2002
Accepted: 23 April 2002
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