* Department of Pathology and Department of Anatomy and Cell Biology, Columbia University College of Physicians and
Surgeons, New York 10032; and § Department of Biology, Queen's University, Kingston, Ontario, Canada, K7L 3N6
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Abstract |
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The budding yeast contains two type I myosins, Myo3p and Myo5p, with redundant functions. Deletion of both myosins results in growth defects, loss of
actin polarity and polarized cell surface growth, and accumulation of intracellular membranes. Expression of
myc-tagged Myo5p in myo3 myo5
cells fully restores
wild-type characteristics. Myo5p is localized as punctate, cortical structures enriched at sites of polarized
cell growth. We find that latrunculin-A-induced depolymerization of F-actin results in loss of Myo5p patches. Moreover, incubation of yeast cells at 37°C results in
transient depolarization of both Myo5p patches and the
actin cytoskeleton. Mutant Myo5 proteins with deletions in nonmotor domains were expressed in myo3
myo5
cells and the resulting strains were analyzed for Myo5p function. Deletion of the tail homology 2 (TH2)
domain, previously implicated in ATP-insensitive actin
binding, has no detectable effect on Myo5p function. In
contrast, myo3
myo5
cells expressing mutant Myo5
proteins with deletions of the src homology domain 3 (SH3) or both TH2 and SH3 domains display defects including Myo5p patch depolarization, actin disorganization, and phenotypes associated with actin dysfunction. These findings support a role for the SH3 domain
in Myo5p localization and function in budding yeast.
The proline-rich protein verprolin (Vrp1p) binds to the
SH3 domain of Myo3p or Myo5p in two-hybrid tests,
coimmunoprecipitates with Myo5p, and colocalizes
with Myo5p. Immunolocalization of the myc-tagged
SH3 domain of Myo5p reveals diffuse cytoplasmic staining. Thus, the SH3 domain of Myo5p contributes
to but is not sufficient for localization of Myo5p either
to patches or to sites of polarized cell growth. Consistent with this, Myo5p patches assemble but do not localize to sites of polarized cell surface growth in a
VRP1 deletion mutant. Our studies support a multistep
model for Myo5p targeting in yeast. The first step, assembly of Myo5p patches, is dependent upon F-actin,
and the second step, polarization of actin patches,
requiresVrp1p and the SH3 domain of Myo5p.
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Introduction |
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THE myosin superfamily of molecular motors has a
crucial role in actin-dependent processes in all eukaryotic cells. This superfamily consists of conventional (type II or muscle-like) myosins and at least ten
classes of unconventional myosins (for review see Cheney
and Mooseker, 1995; Sellers and Goodson, 1995
). All unconventional myosin proteins have conserved "head" and
"neck" regions. The head or motor domain contains binding sites for ATP and actin. The necks of myosin proteins
have a variable number of isoleucine- and glutamine-rich
IQ motifs, possible binding sites for calmodulin or calmodulin-like light chains. The carboxy-terminal "tail" regions, which are highly divergent among classes of unconventional
myosins, contain sequences implicated in protein-protein
interactions, membrane binding, and intracellular signaling.
The type I myosin proteins were the first unconventional myosins to be discovered and remain the best studied class (Pollard and Korn, 1973). Representatives of this
class are present in phylogenetically diverse organisms including filamentous fungi, ameba, yeast, and vertebrates.
This points to a central role for these proteins in eukaryotic cell function (for review see Cheney and Mooseker,
1995
). Phylogenetic sequence comparisons of the head regions of a host of novel myosins-I reveals at least four subclasses. Our study focuses on the subclass of myosins-I
known as "classic" or "ameboid" myosins-I.
In Saccharomyces cerevisiae, there are two highly related classic type I myosin isoforms, MYO3 and MYO5,
which appear to have overlapping activities (Goodson and
Spudich, 1995; Goodson et al., 1996
). Deletion of MYO3
or MYO5 alone leads to only subtle defects. In contrast,
deletion of both MYO3 and MYO5 results in severe defects in actin organization and phenotypes associated with
actin cytoskeletal dysfunction including slow growth, accumulation of intracellular membranes and vesicles, cell
rounding, random bud site selection, and impaired secretion and endocytosis (Geli and Riezman, 1996
; Goodson
et al., 1996
). Myo5p is localized as punctate, cortical structures that are enriched at sites of polarized growth and actin cytoskeletal polarization. These observations support a
general role for Myo5p in organization of the yeast actin
cytoskeleton.
Recent studies also support a role for the classic myosin
I of Dictyostelium discoideum, myoB, in control of actin
organization (Temesvari et al., 1996). Moreover, other
classic myosin I proteins have been implicated in a variety
of processes that are dependent upon actin cytoskeletal
function (Jung and Hammer, 1990
; Jung et al., 1993
, 1996
;
Titus et al., 1993
; Peterson et al., 1995
; Novak et al., 1995
;
McGoldrick et al., 1995
; Goodson et al., 1996
). myoB,
myoC, and myoD of Dictyostelium contribute to endocytosis, motility, streaming, and phagocytosis (Jung and
Hammer, 1990
; Titus et al., 1993
; Jung et al., 1996
). myoA,
a classic type I myosin that is essential for viability in Aspergillus nidulans, is required for polarized growth and secretion (McGoldrick et al., 1995
). Finally, myosin IC of
Acanthamoeba castellani is essential for contractile vacuole function (Doberstein et al., 1993
).
Consistent with this, classic myosin I proteins in yeast
and other organisms (Fukui et al., 1989; Jung et al., 1993
;
Baines et al., 1995
; McGoldrick et al., 1995
; Goodson et al.,
1996
) are localized to actin-rich regions or sites of actin reorganization. First, many other myosin I proteins including myosin IC of Acanthamoeba, myoA and B of Dictyostelium, and myoA of Aspergillus display cortical localization.
Second, classic myosin I proteins are localized at sites of
actin organization in budding yeast, Aspergillus, and Dictyostelium. Third, the classic myosin I protein of Aspergillus occurs in punctate structures similar to cortical Myo5p
patches of budding yeast.
Here, we report studies on the role of the tail homology
domain 2 (TH2)1 and src homology domain 3 (SH3) domains of the Myo5p tail in Myo5p-control of actin organization and function in vivo. These regions are present in
all classic myosin I proteins; however, their contribution to
myosin I function within the context of the cell is not well
understood (for review see Sellers and Goodson, 1995).
The TH2 domain is a glycine-proline-alanine-rich region implicated in ATP-insensitive actin binding and actin
cross-linking in vitro (Lynch et al., 1986
; Pollard et al.,
1991
; Jung and Hammer, 1994
; Rosenfeld and Rener,
1994
). The SH3 domain, a region that binds proline-rich
sequences, has been implicated in a variety of cell functions. Early studies indicate that the SH3 domain mediates
protein-protein interactions that regulate enzymatic activity. For example, binding of p85 to the SH3 domain of
phosphatidylinositol-3' kinase (PI-3 kinase) increases PI-3
kinase activity five- to sevenfold (Pleiman et al., 1994
).
Since classic myosin I proteins contain proline-rich regions
within their TH2 domain, it is possible that their SH3 domain participates in intramolecular links which regulate function (Goodson and Spudich, 1995
). Other studies indicate that the SH3 domain contributes to protein targeting.
For example, SH3 domains of the NADPH oxidase subunits, p47phox and p67phox, mediate protein-protein interactions required to recruit p47phox and p67phox to the
membrane to form an activated oxidase (de Mendez et al.,
1994
, 1996
). Moreover, microinjection studies with the SH3 domain of phospholipase C-
indicate that the SH3
domain mediates localization of phospholipase C-
to the
microfilament network (Bar-Sagi et al., 1993
). Indeed,
many cytoskeleton-associated proteins including myosin I
(Bement et al., 1994
; Stoffler et al., 1995
; Goodson and
Spudich, 1995
; Goodson et al., 1996
), fodrin (Merilainen
et al., 1993
), nebulin (Wang et al., 1996
), cortactin (Wu
and Parsons, 1993
), and the actin-associated proteins of
yeast Abp1p (Drubin et al., 1990
; Lila and Drubin, 1997
),
Rvs167p (Bauer et al., 1993
), Bem1p (Chenevert et al.,
1993), Boi1p and Boi2p (Bender et al., 1996
; Matsui et al.,
1996
) contain SH3 domains, which underscores the importance of the SH3 domain in protein-protein interactions
with the cytoskeleton.
We find that the SH3 domain is critical for Myo5p patch polarization and Myo5p-dependent actin organization, and identify Vrp1p as a likely binding partner for the SH3 domain of yeast myosin I proteins. Moreover, we find that targeting of Myo5p in yeast is a multistep process.
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Materials and Methods |
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Yeast and Bacterial Manipulations
S. cerevisiae strains used in this study are the following: (a) HA10-1b
(wild-type), MAT can1-100 ade2-1 his3-11 leu2-3,112 ura3-1 trp1-1
(Goodson and Spudich, 1995
); (b) HA31-9c (myo3
myo5
), MATa
can1-100 ade2-1 his3-11 leu2-3,112 ura3-1 trp1-1 myo3::HIS3 myo5::TRP1;
(d) HA31-9a (myo3
), and (e) MAT
can1-100 ade2-1 his3-11 leu2-3,112
ura3-1 trp1-1 myo3::HIS3 (Goodson et al., 1996
). VHA-1 is an HA31-9c-
derived strain in which the DNA encoding three copies of the hemagglutinin (HA) tag was inserted into the 3' end of the chromosomal copy of the
VRP1 gene (see below). Two-hybrid assays were carried out using the
strain Y704 (MATa lexAop-LEU2 lex-Aop-lacZ sst1
his3 trp1 ura3-52 leu2). The effect of verprolin deletion on Myo5p localization was evaluated in the vrp1
strain (GVY1 - MAT
/MATa leu2-3,112/leu2-3,112 ade1/ade1 ura3-52/ura3-52 Ile-/Ile- MEL1/MEL1 vrp1::LEU2/rrp1:: LEU2) and the corresponding wild-type strain (Sc467-MAT
/MATa leu2-3,112/leu2-3,112 ade1/ade1 ura3-52/ura3-52 Ile-/Ile- MEL1/MEL1). Yeast manipulations including cell culture, transformation, and tetrad
analysis were carried out according to Guthrie and Fink (1991)
. Bacterial
manipulations were carried out according to Sambrook et al. (1989)
.
Construction of Plasmids Containing Mutant myo5 Constructs
Expression vectors for these studies will be referred to using the following
convention: (a) pMYO5, plasmid-borne wild-type MYO5 gene bearing
three copies of the myc epitope at its carboxy terminus; (b) pTH2, plasmid-borne myc-tagged MYO5 gene bearing a deletion of the TH2 region;
(c) pSH3
, plasmid-borne myc-tagged MYO5 gene bearing a deletion of
the SH3 region; (d) pTH2
-SH3
, plasmid-borne myc-tagged MYO5
gene bearing deletions of the TH2 and SH3 regions; and (e) pSH3, plasmid-borne myc-tagged SH3 domain of the MYO5 gene.
All myo5 containing plasmids used in this study were generated using
the pRS-Y2 MYO5-myc construct described in our previous work (Goodson et al., 1996). pTH2
was created using the Transformer Site-Directed
Mutagenesis Kit from Clontech Laboratories, Inc. (Palo Alto, CA) according to the manufacturer's protocol. The selection oligonucleotide
primer used for this construct (5'-GCGGTGGCGGTCGCTCTAGAAC-3') destroys a unique NotI site in the noncoding region of the vector. The
mutagenic primer used was 5'GCAGCCCAGCATGTTAAAGAACCCATGTTGAAGC-3'. The pSH3
and pTH2
-SH3
constructs were
created using overlapping PCR products with site-directed mutations. For
pSH3
and pTH2
-SH3
constructs, the outer primers used were 5'-CTCGAGGTCGACGGTATCGA-3' and 5'-CAATATCAGTTCGTCGTGG-3'. The inner primers were 5'-CCAAAAGAACCCATGTTTGAAAAACCACATTCCGGAAATAATAATATC-3' and 5'-GATATTATTATTTCCGGAATGTGGTTTTTCAAACATGGGTTCTTT-
TGG-3' for pSH3
and 5'-GCTGCAGCCCAGCATGTTAAACCACATTCCGGAAATAATAATATC-3' and 5'-GATATTATTATTTCCGGAATGTGGTTTAACATGCTGGGCTGCAGC-3' for pTH2
-SH3
. The
full-length PCR products from the pSH3
and pTH2
-SH3
mutations were cloned in place of the endogenous BstEII-SalI fragment of pRS-Y2
MYO5-myc.
The plasmid containing the SH3 domain of MYO5 (pSH3) was constructed by PCR using the primers 5'-TTATGTGGCCAATACGAATTTAACCGCTTTATAGAAATGGCTATCCCAAAAGAACCCA-TGTTTGAAGCGGCTTACG-3' and 5'-GACCATGATTACGCCAAGCTC-3'. The PCR product was digested with MscI and XhoI and cloned in place of the endogenous MscI-XhoI fragment of pRS-Y2 MYO5-myc. All constructs were confirmed by DNA sequencing.
Construction of Plasmids for Two-Hybrid Analysis
To create p1360, carrying a DNA fragment coding for Vrp1p (1-200), we amplified a 0.6-kb fragment of VRP1 by PCR with primers (5'-GGATCCAAATGGCAGGTGCTCCAGCTCCT-3') and (5'-GCGGCCGCTCACCTTACTGAAGGCATATGCGG-3') that incorporated BamHI and NotI sites. The product was ligated into pCR-Script/SK+ (Stratagene, La Jolla, CA). To create p1361, carrying a DNA fragment coding for Vrp1p (195-817), we amplified a 1.9-kb fragment of VRP1 by PCR with primers (5'-GGATCCATATGCCTTCAGTAAGGCCAGCAC-3') and (5'-GCGGCCGCTCACGTAAATAATGTTAAGTCCAATGGCACACTACT-AC-3') that incorporated BamHI and NotI sites. The product was ligated into pCR-Script/SK+ (Stratagene, La Jolla, CA).
To create p1645, carrying a DNA fragment coding for Myo3p (1,118-1,271), we amplified a 0.5-kb fragment of MYO3 by PCR with primers (5'-CAACCAAAGGATCCGAAATTCGAAGCT-3') and (5'-TCGCGACCAGTCATCATCATC ATCGCCATCGT-3') that used an internal BamHI site in the MYO3 sequence and incorporated an NruI site before the stop codon of the MYO3 sequence, and then the product was ligated into pCR 2.1 TOPO (Invitrogen, Carlsbad, CA). p1686 is similar to p1645 except that the NruI site is replaced by a stop codon followed by a NotI site. p1705 contains a BamHI to NotI fragment encoding for Myo3p (1,118- 1,271) cloned into KS+ (Stratagene). To create p1408, carrying a DNA fragment coding for Myo5p (1,082-1,219), we amplified a 0.4-kb fragment of MYO5 by PCR with primers (5'-TGGATCCGCGAATCAAAGCCAAAAGAACCCATGTTTGAAG-3') and (5'-TGCGGCCGCTGATTACCAATCATCTTCCTCTTCATCT-3') that incorporated BamHI and NotI sites and then the product was ligated into pCR-Script/SK+.
Two-hybrid constructs were based on pEG202 (Gyuris et al., 1991),
which encodes the LexA DNA-binding domain, pJG4-5 (Gyuris et al.,
1991), which encodes the B42 transcription activation domain, and pACT
(Durfee et al., 1993), which encodes the Gal4p transcription activation domain. pEG202-derived plasmids used were p1386, which contains a
BamHI to NotI fragment encoding Vrp1p (1-200); p1388, which contains
a BamHI to NotI fragment encoding Vrp1p (195-817); p1707, which contains a BamHI to NotI fragment encoding Myo3p (1,118-1,271); and
p1456, which contains a BamHI to NotI fragment encoding Myo5p
(1,082-1,219). pJG4-5-derived plasmids used were p1387, which contains
a BamHI to NotI fragment encoding Vrp1p (1-200); p1389, which contains a BamHI to NotI fragment encoding Vrp1p (195-817); p1706, which
contains a BamHI to NotI fragment encoding Myo3p (1,118-1,271);
p1457, which contains a BamHI to NotI fragment encoding Myo5p
(1,082-1,219). p1962 is a pACT-derived plasmid that encodes full-length
Vrp1p (Vaduva et al., 1997
).
Epitope Tagging of Vrp1p
Vrp1p was tagged at its carboxy terminus with three copies of the peptide
epitope from the HA protein of human influenza virus using a derivative
of a gene disruption cassette (Wach, 1996; Longtine et al., 1998
). This cassette codes for three copies of the HA epitope and the heterologous dominant resistance marker, kanMX, which confers geneticin (G418) resistance in yeast. The cassette with long-flanking VRP1 homology regions
was produced using PCR amplification and the forward and reverse primers: 5'-AAAGGGTAGTAGTGTGCCATTGGACTTAACATTATTTACGCGGATCCCCGGGTTAATTAA-3' and 5'-TGTTCTTCAGTGATTTATTGTAACCATGGAGAAATGCTCAGAATTCGAGCTCG TT T - AAAC-3. myo3
myo5
cells bearing pMYO5 were transformed with the
PCR product. Cells bearing the integrated cassette were selected by
growth on rich, glucose-based media (YPD) plates containing 200 mg/liter
of G418 (Sigma Chemical Co., St. Louis, MO). HA tagging of Vrp1p was
confirmed by Western blot analysis using an anti-HA antibody (clone
3F10; Boehringer Mannheim, Indianapolis, IN). HA-tagged Vrp1p is a 93-kD protein based on its amino acid content. However, HA-Vrp1p migrates on SDS gels as a 120-kD protein, presumably due to its high proline
content.
Light Microscopy
Actin cytoskeletal structure and chitin deposition were visualized using
rhodamine-phalloidin, a rabbit polyclonal antibody raised against yeast
actin (Drubin et al., 1988), and calcofluor (Sigma Chemical Co.) according
to published procedures (Adams et al., 1991
; Pringle et al., 1991
). Immunofluorescence detection of myc-tagged Myo5p was carried out as described
previously (Goodson et al., 1996
). HA-tagged Vrp1p was visualized using
a rat monoclonal antibody (see above), and FITC-coupled goat anti-rat
antibody (Kirkegaard and Perry Laboratories, Inc., Gaithersburg, MD).
Other methods (fixation, mounting, and DAPI staining) were as described by Pringle et al. (1991)
. Photomicroscopy was performed on a Leitz Dialux microscope (Rockleigh, NJ) and a Zeiss Axioplan II microscope (Oberkochen, Germany). Images were collected using a cooled charge-coupled device camera (model Star-1; Photometrics, Tucson, AZ). Light output from the 100-W mercury arc lamp was controlled using a shutter
driver (model Uniblitz D122; Vincent Associates, Rochester, NY) and attenuated using neutral density filters (Omega Optical Corporation, Brattleboro, VT). Image enhancement and analysis were performed on a Macintosh Quadra 800 computer (Cupertino, CA) using the public domain
program NIH Image 1.60 (National Institutes of Health, Bethesda, MD).
Images were stored on a magnetic optical disk drive (Peripheral Land
Inc., Fremont, CA).
Electron Microscopy
Preparation of samples for transmission electron microscopy was carried
out according to Stevens (1977). Yeast were fixed by addition of glutaraldehyde (Sigma Chemical Co.) to growth medium to a final concentration
of 5%. After incubation for 3 h at room temperature (RT), cells were concentrated by centrifugation at 10,000 g for 10 min at RT and then washed
two times with 0.9% NaCl. Samples were resuspended in 4% KMnO4 in
0.1 M Na-cacodylate, pH 7.4 (Electron Microscopy Sciences, Fort Washington, PA), and incubated at 4°C for 1 h with gentle rotation. After two
washes with 0.9% NaCl, samples were resuspended in 2% uranyl acetate (Electron Microscopy Sciences) and then incubated for 1 h at RT. Samples were washed three times, dehydrated in a graded series of ethanol solutions, infiltrated with propylene oxide for 10 min, and then embedded in
Epon-812 (Tousimis Research Co., Rockville, MD). Ultrathin sections
were stained for 5 min with 1% lead citrate before viewing with a JEOL
1200 transmission electron microscope (Peabody, MA).
Immunoprecipitation
50-ml cultures of various yeast strains were grown to mid-log phase. Cells
were collected and lysed by vortexing for 6 min at 4°C with 0.5-mm glass
beads in a solution consisting of 10% glycerol, 10 mM EGTA, 1% Triton
X-100, 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM MgCl2, 1 mM ATP,
2 mM PMSF, and protease inhibitor cocktail as described in Lazzarino et al.
(1994). The protein extracts were clarified by centrifugation for 5 min at
10,000 g and the supernatant was used for immunoprecipitation experiments. 200 µl of 2 mg/ml protein extract were incubated with 6 µg of anti-myc antibody for 2 h at 4°C. Thereafter, 15 µl of protein-G Sepharose 4 Fast Flow (Pharmacia Biotech. Inc., Piscataway, NJ) pretreated with BSA
was added. This mixture was further incubated for 1 h at 4°C. The
Sepharose beads were then pelleted and washed two times with a buffer
consisting of 20 mM Tris-HCl, pH 7.5, 10% glycerol, 1% Triton X-100,
150 mM NaCl, 1 mM PMSF, and protease inhibitor cocktail. Proteins recovered on Sepharose beads were separated by SDS-PAGE (Laemmli,
1970
) and analyzed by immunoblots (Towbin et al., 1979
).
Other Methods
To detect mutant and wild-type Myo5p protein expression levels, whole
cell extracts were prepared by vortexing yeast cells with 0.5-mm glass
beads in a solution consisting of 10% glycerol, 10 mM EGTA, 1% Triton
X-100, 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM PMSF, and protease
inhibitor cocktail. Protein concentrations were determined using the
bicinchoninic acid assay following the vendor's protocol (Pierce Chemical
Co., Rockford, IL). Gel electrophoresis and immunoblots were performed
as described above. Latrunculin-A (Molecular Probes, Eugene OR) treatment was carried out as described in Ayscough et al. (1997).
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Results |
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Construction and Expression of myo5 Mutants
Expression of myc-tagged Myo5p on a low-copy number
plasmid under control of the MYO5 promoter (pMYO5)
complements slow growth rate and defects in actin organization and function produced by deletion of endogenous
myosin I genes (Goodson et al., 1996). To investigate the
functional roles of various domains within the tail of
Myo5p, we created MYO5-myc constructs containing deletions of either the TH2, SH3, or TH2 and SH3 domains
(Fig. 1). We defined the TH2 domain as amino acids (aa)
1,000-1,091, a region enriched in glycine, proline, and alanine that is amino-terminal to the SH3 domain. However,
in some Acanthamoeba and Dictyostelium myosin I proteins, the TH2 region is believed to be bipartite: part of the
TH2 region is amino-terminal to the SH3 domain, and part
is carboxy-terminal to the SH3 domain. Since a proline-
and alanine-rich region is present carboxy-terminal to the
SH3 domain in Myo5p (aa 1,141-1,200), it is formally possible that the TH2 region of yeast myosin I proteins extends beyond the region deleted in this study. The SH3 domain of MYO5 encompasses aa 1,092-1,140. We expressed
these mutant myosin I constructs using low-copy, centromere-based plasmids and the endogenous MYO5 promoter in cells bearing deletions of endogenous myosin I
genes, and then examined the effect of mutant myosin I
proteins on actin organization and function in vivo.
|
Expression of various epitope-tagged and untagged
Myo5p in myo3 myo5
mutants was examined by Western blot analysis using an anti-myc antibody (Evan et al.,
1985
) and an antibody raised against a myosin I-specific
peptide (Ruppert et al., 1993
) (Fig. 2). Western blot analysis using a myosin I-specific antipeptide antibody indicated
that the level of expression of plasmid-borne wild-type
and mutant Myo5p in the myo3
myo5
cells is similar to
that of endogenously expressed myosin I proteins in wild-type cells. Moreover, myc-tagged myosin I proteins of the
expected electrophoretic mobility were detected in cells
expressing wild-type and mutant MYO5 genes. The level
of expression of Myo5p bearing deletions in both SH3 and
TH2 domains is lower than that of wild-type Myo5p. However, the level of the other mutant proteins was comparable to that of protein expressed from pMYO5.
|
Effect of Mutant myo5 Constructs on Cell Growth
Deletion of MYO3 and MYO5 results in severe growth defects. The doubling time of the myosin I double deletion
mutant in liquid culture at 30°C is two to three times
greater than that of wild-type cells or myosin I single mutants (Goodson et al., 1996). In addition, myo3
myo5
cells do not grow on solid rich media at 37°C or at high osmotic strength (0.75 M KCl) (Goodson et al., 1996
). Expression of pMYO5 in myo3
myo5
cells fully restores
growth under all of the restrictive conditions (Goodson et al.,
1996
; Fig. 3 and Table I).
|
|
The growth of pTH2-expressing cells is indistinguishable from that of wild-type cells or of pMYO5-rescued myosin double mutants (Fig. 3 and Table I). In contrast, deletion of the SH3 domain from MYO5 results in defects in
Myo5p function (Fig. 3 and Table I). Although pSH3
-
expressing cells grow under all conditions tested, the extent of growth on solid medium at 37°C or at high osmotic
strength, and the rate of growth in liquid culture are significantly lower than those of wild-type cells or myo3
myo5
cells rescued with pMYO5. Unlike the myo3
myo5
cells,
which exhibit a large increase in doubling time after temperature shift to 37°C, the pSH3
-expressing cells grow at
37°C as efficiently as at 30°C (Table I). Although deletion
of the TH2 domain of Myo5p does not have any significant
effect on growth, deletion of both the TH2 and SH3 domains produces a growth rate slightly lower than that observed after deletion of the SH3 domain alone (Fig. 3 and
Table I). We attribute this decrease in growth rate to the
fact that the expression level of mutant Myo5p bearing both TH2 and SH3 deletions is somewhat lower than that
of all other expression constructs.
Effect of Mutant myo5 on Repolarization of the Actin Cytoskeleton after Temperature-induced Depolarization
Actin patches and cables are the two F-actin-containing
structures detected by light microscopy in budding yeast.
In wild-type yeast, patches are concentrated exclusively in
buds and cables are aligned along the mother-bud axis
(Kilmartin and Adams, 1984). This polarization of actin
cytoskeletal structures is transiently disrupted by temperature shift. Transfer of wild-type yeast cultures from 30° to
37°C for 30 min causes loss of actin cables and delocalization of actin patches over both mother and bud cell surfaces. Polarization of actin cables and patches is restored
within 90 min after the temperature shift (Lillie and
Brown, 1994
; Fig. 4 A, panels A, D, and G).
|
Actin patches and cables are present in myo3 myo5
mutants. However, deletion of yeast myosin I genes results
in loss of actin polarization in over 95% of the cells examined during vegetative growth at 30°C (Goodson et al.,
1996
; Fig. 5). This depolarization persists through the temperature shift (Fig. 4 A, panels B, E, and H). Expression of
pMYO5 in these cells restores organization of the actin cytoskeleton during vegetative growth at 30°C (Goodson et al.,
1996
), and the normal time course of actin repolarization after temperature-induced depolarization (Fig. 4 A, panels
C, F, and I).
|
We examined the actin polarization and time course of
actin repolarization in response to temperature shift in
pTH2-, pSH3
- and pTH2
-SH3
-expressing cells (Figs.
4 and 5). Actin organization during growth at 30°C, and
the kinetics of actin repolarization after shift to 37°C, are
not significantly altered in pTH2
-expressing cells compared with double deletion mutants expressing pMYO5
(Fig. 4 B, panels A, D, and G). In contrast, actin organization during vegetative growth at 30°C and the kinetics of
actin reorganization are altered in pSH3
- and pTH2
-
SH3
-expressing cells (Fig. 4 B, panels B, E, and H and C,
F, and I, respectively). Depolarization of actin is observed
in 20-30% of pSH3
- or pTH2
-SH3
-expressing cells
even at 30°C (Fig. 5). Moreover, only 44-48% of these cells display repolarization of the actin cytoskeleton 90 min after temperature shift. For comparison, ~85% of the cells expressing pMYO5 or pTH2
display actin repolarization
under similar conditions. Therefore, the SH3 domain of
Myo5p is required for full Myo5p function in actin organization.
Effect of Mutant myo5 on Actin-dependent Activities
Phenotypic analysis of yeast bearing mutations in actin
(ACT1) or in the actin-binding proteins fimbrin (SAC6),
capping protein (CAP1, 2), profilin (PFY1), conventional
myosin (MYO1), and myosin V (MYO2) suggest a role for
the actin cytoskeleton in control of cell shape, bud site selection, secretion, endocytosis, cell wall and chitin deposition, growth at high osmotic strength, and mitochondrial
organization (Novick and Botstein, 1985; Haarer et al.,
1990; Rodriguez and Patterson, 1990; Adams et al., 1991; Johnston et al., 1991; Amatruda et al., 1992; Chowdhury
et al., 1992; Liu and Bretscher, 1992
; Drubin et al., 1993;
Kübler and Riezman, 1993
; Lazzarino et al., 1994; Simon
et al., 1995). To determine whether the defects in actin
structure observed in cells expressing myo5 mutations also
produce defects in actin-dependent functions, we studied
the effect of myo5 mutations on mitochondrial, nuclear,
and vesicular organization and on chitin and cell wall deposition (Table II).
|
Wild-type cells and myo3 myo5
mutants that express
pMYO5, or pTH2
all display normal mitochondrial morphology: mitochondria in these cells are extended, tubular
structures colocalizing with actin cables. These cells also
display normal cell walls, normal chitin rings at bud sites,
and no significant accumulation of intracellular membrane
or vesicle. In contrast, pSH3
- or pTH2
-SH3
-expressing cells display (a) mitochondrial aggregation; (b) accumulation of small (50-nm) vesicles and large (200-500-nm
diam) multilamellar structures that resemble the Berkeley
bodies previously observed in late secretory mutants
(Novick et al., 1981
); (c) abnormal, delocalized chitin deposition over the surface of the mother cell, and abnormal,
brightly stained patches of chitin irregularly deposited in
the cell walls; and (d) thickened cell wall (Table II). The defects in actin-dependent processes in pSH3
- and
pTH2
-SH3
-expressing cells are not as severe as those
in myosin I double deletion cells expressing vector alone.
Thus, the observed partial defects in actin organization described above correlate with partial defects in actin function.
Effect of Actin Depolarization and Depolymerization on Myo5p
In wild-type cells grown at 30°C, immunostaining of myc-tagged Myo5p reveals punctate staining and polarization, i.e., enrichment in the bud at sites of polarized cell surface growth (Fig. 6 B). Myo5p patches show cell cycle dependent polarization that correlates with actin patch polarization. In cells bearing small- and medium-sized buds, actin patches (Fig. 6 A) and Myo5p patches (Fig. 6 B) are enriched in the bud. There is a correlation between actin patch depolarization and Myo5p depolarization in cells bearing large buds (Table III). 30 min after the temperature shift, concomitant with loss of actin polarization (Fig. 6 C), Myo5p patch localization is clearly altered. At this time point, Myo5p staining is still punctate; however, Myo5p patches are delocalized throughout both mother and daughter cells (Fig. 6 D). Finally, Myo5p patch repolarization occurs concomitant with repolarization of actin patches; both are complete within 90 min after the temperature shift (Fig. 6, E and F). This correlation between polarization of actin and Myo5p patches is consistent with a role of Myo5p in actin organization.
|
|
To evaluate the role of F-actin depolymerization in
Myo5p patch assembly and polarization, we treated cells
with latrunculin-A (LAT-A; Spector et al., 1983; Ayscough et al., 1997
). Treatment of myo3
myo5
cells expressing pMYO5 with LAT-A for 15 min causes complete
loss of F-actin as assessed by rhodamine-phalloidin staining (Fig. 7, B and D). This treatment also causes a significant decrease in the intensity of Myo5p staining in patches,
suggesting a defect in Myo5p patch assembly or stabilization. In addition, Myo5p patches which are present in
LAT-A-treated cells show depolarization (Fig. 7, A and
C). Over 90% of control cells treated with the same concentration of DMSO as that used for LAT-A treatment
display enrichment of Myo5p patches in the bud of cells
bearing small- and medium-sized buds. In contrast, only
12.5% of the LAT-A-treated cells examined display polarized Myo5p patches in cells bearing small- and medium-sized buds. Although polarization of Myo5p was observed
in some LAT-A-treated cells, the level of enrichment of
Myo5p in buds of LAT-A-treated cells was not as great as
that observed in controls.
|
The SH3 Domain of Myo5p Is Required but Not Sufficient for Localization of Myo5p at Sites of Polarized Cell Surface Growth
To determine whether the TH2, SH3, or both domains
have a role in Myo5p targeting, we examined the localization of proteins expressed from pMYO5, pTH2, pSH3
,
and pTH2
-SH3
by indirect immunofluorescence (Fig.
8). In all cases, staining of wild-type and mutant Myo5p
was punctate and cortical (Fig. 8, B, D, F, and H). Moreover, patches containing TH2-deleted Myo5p resembled
wild-type Myo5p patches and were enriched in the bud
(n = 74). In contrast, patches containing Myo5p bearing
deletions in the SH3 domain alone, or in the TH2 and SH3
domains, were evenly distributed in mother cells and bud:
only 3% of cells examined showed enrichment of these
proteins in the bud (n = 101 and n = 111, respectively; Fig.
8, F and H). Although partial depolarization of the actin cytoskeleton is observed in pSH3
- and pTH2
-SH3
-expressing cells, depolarization of Myo5p patches in these
cells is not due to effects on actin organization. Patches containing mutant Myo5p protein are delocalized even in
pSH3
- and pTH2
-SH3
-expressing cells that contain
polarized actin patches and cables.
|
According to the results described above, the SH3 domain is required for targeting of Myo5p to growing buds.
To examine the role of this domain in (a) assembly of
Myo5p into patches, and (b) enrichment of Myo5p patches
at sites of polarized cell surface growth, we constructed a
plasmid, pSH3, carrying the SH3 domain and the subsequent 79-aa carboxy terminus of Myo5p (aa 1,086-1,219) fused to three copies of the myc epitope. This construct
was expressed and localized in a MYO3 deletion mutant.
myo3 cells were used for expression because they contain a polarized actin cytoskeleton, and only one of two
endogenous myosin I genes. Western blot analysis using
the anti-myc antibody indicates that expression of pSH3 using a low-copy plasmid and the endogenous MYO5 promoter produces a protein of the expected apparent molecular weight at levels comparable to that of protein expressed
from pMYO5 (data not shown). Immunolocalization of
this fusion protein using the anti-myc antibody reveals diffuse staining which is distributed throughout the cytoplasm (Fig. 9). Thus, the SH3 domain of Myo5p is not sufficient for either association of Myo5p with patches or for
enrichment of Myo5p patches at sites of polarized cell surface growth.
|
The Proline-rich, SH3-binding Protein, Verprolin, Is a Myo5p Binding Partner
In two-hybrid tests for protein-protein interaction, the
SH3 domains of Myo3p and Myo5p bound a 10-residue
polyproline sequence (Evangelista, M., and C. Boone, unpublished data). With a short polyproline sequence as a
probe, we performed a BLAST search (Altschul et al.,
1990) against the translated yeast genome to identify potential binding partners for the SH3 domain of yeast myosin I proteins. This search highlighted verprolin, Vrp1p, an
actin-binding protein implicated in actin cytoskeletal organization and function (Donnelly et al., 1993
; Vaduva et al.,
1997
). Vrp1p contains several short stretches of polyproline and 11 copies of a potential SH3-binding motif with
the general sequence APPL/IP (Bar-Sagi et al., 1993
). We
therefore evaluated interactions between Vrp1p and the
SH3 domain of yeast type I myosins using the two-hybrid
system (Table IV). We find that both the Myo3p and
Myo5p SH3 domains interact with full-length Vrp1p and
with two smaller Vrp1p fragments, Vrp1p (1-200) and Vrp1p
(195-817). Thus, the SH3 domains of the yeast myosin I
proteins may bind to multiple sites within Vrp1p.
|
If Vrp1p and Myo5p interact in vivo, then these two proteins should colocalize. Localization of Vrp1p was performed by tagging the chromosomal copy of the VRP1
gene at its carboxy terminus with three copies of the HA
epitope. We find that carboxy-terminal tagging has no effect on Vrp1p function; growth rates (data not shown) and
actin cytoskeletal organization (Fig. 10 B) of the HA-Vrp1p expressing cells are indistinguishable from those of
cells expressing untagged Vrp1p. This observation is consistent with the report that addition of GFP to the carboxy-terminus of Vrp1p does not affect Vrp1p function
(Vaduva et al., 1997). Visualization of HA-tagged Vrp1p
confirmed the observation that Vrp1p is localized to punctate structures enriched at sites of polarized cell surface growth (Vaduva et al., 1997
). In addition, we find that
Myo5p patches colocalize with Vrp1p patches (Fig. 10, C
and D).
|
Consistent with this, we find that Vrp1p is required for
polarization of both Myo5p patches and the actin cytoskeleton. A vrp1 strain and the corresponding wild-type parent which express myc-Myo5p were fixed and stained for
Myo5p and the actin cytoskeleton. As described previously, VRP1 deletion causes actin cytoskeletal depolarization (Vaduva et al., 1997
). Our studies confirm this observation, and show that Myo5p patches are polarized in the wild-type strain (Fig. 10, E and F) and depolarized in the
vrp1
cell (Fig. 10, G and H).
Finally, to test for direct interactions between Vrp1p and Myo5p in yeast, myc-tagged Myo5p was immunoprecipitated from extracts of cells that express myc-tagged Myo5p and HA-tagged Vrp1p. The immunoprecipitates were analyzed by Western immunoblotting using probes for myc and HA (Fig. 11). These reveal that Vrp1p coimmunoprecipitates with Myo5p. This coimmunoprecipitation is specific; it occurs only in cells that express both tagged constructs, and is antibody dependent. Our findings that Vrp1p (a) has the capacity to bind to the SH3 domain of Myo5p, (b) colocalizes with Myo5p, (c) is required for polarization of both Myo5p patches and the actin cytoskeleton, and (d) coimmunoprecipitates with Myo5p support a role for Vrp1p as a Myo5p binding partner.
|
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Discussion |
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---|
Type I myosin proteins have been identified in every eukaryotic cell studied. However, we are only beginning to
understand the roles of these proteins in actin-dependent
processes. In the budding yeast Saccharomyces cerevisiae,
we have evidence that a primary function of classic type I
myosins is the control of actin cytoskeletal organization
(Goodson et al., 1996). Consistent with this, we observe a
correlation between polarization of the actin cytoskeleton
and that of Myo5p patches. Incubation of yeast cells at
37°C produces transient depolarization of both the actin cytoskeleton and Myo5p patches. Moreover, subsequent
repolarization of the actin cytoskeleton occurs concomitant with Myo5p patch repolarization.
In the present study, we also examined the role of myosin I tail domains in the physiological function of the protein. To do so, we expressed mutated forms of the yeast
type I myosin MYO5 in myo3 myo5
cells and examined
the extent to which these mutant myosins can rescue wild-type phenotypes. From this, we have drawn additional
conclusions concerning the nature of type I myosin function, and the role of tail domains in myosin I complex assembly and targeting.
In vitro data suggest that classic myosin I proteins have the capacity to bind and cross-link actin using the ATP- insensitive actin binding site in the TH2 domain of their tail and the ATP-sensitive actin binding site in their head, and may therefore organize microfilament networks by translocating actin structures in relation to other actin filaments (refer to Introduction). However, we find that a mutant Myo5p containing a deletion of the putative ATP-insensitive actin binding site in the TH2 domain is fully functional under the experimental conditions tested: growth rates, actin organization and repolarization subsequent to temperature shift, and actin-dependent processes including mitochondrial organization, chitin deposition, cell shape, and cell wall deposition are all unaffected. Additionally, TH2-deleted Myo5p is correctly assembled into patches and targeted to sites of polarized cell surface growth. Apparently, partial truncation of the tail alone does not have any functional consequences and the TH2 domain, as defined, is dispensable for the myosin I function in actin organization. Alternatively, since a proline- and alanine-rich region is present carboxy-terminal to the SH3 domain in Myo5p (see above), it cannot be excluded that deletion of this region might lead to alteration in Myo5p behavior.
In contrast to our observations with the TH2 domain,
we find that the SH3 domain of the myosin I tail is required for optimal Myo5p function in yeast: expression of
mutant MYO5 genes bearing deletions in either the SH3
domain or the SH3 and TH2 domains do not fully complement loss of MYO3 and MYO5. pSH3- and pTH2
- SH3
-expressing cells have defects in actin organization
and reorganization in response to temperature stress. In
addition, these cells grow more slowly in liquid culture and
are sensitive to high temperature (37°C) or to high osmotic
strength. Finally, these cells display phenotypes of actin
disorganization including aggregation of mitochondria, multinucleation, thickened cell walls, and accumulation of
intracellular membranes. The extent of defects in pTH2
-
SH3
-expressing cells is similar to that in pSH3
-expressing cells. We attribute the modest difference in the growth
rates of these cells to the fact that the level of Myo5p expressed from pTH2
-SH3
is slightly lower than that
from pSH3
. These findings support the model that the
TH2 domain is dispensable for myosin I function, and argue against a role for intramolecular interactions between
the SH3 and TH2 domains of Myo5p in Myo5p regulation.
Moreover, our data indicate that the SH3 domain is required for normal Myo5p function, consistent with the
finding that the SH3 domain of the classic myosin I of Dictyostelium, myoB, may be required for myoB function in
cell migration (Novak and Titus, 1997
).
The SH3 domain, a module which mediates protein-
protein interactions, has been postulated to have diverse
functions including control of protein activity, localization,
and association with the cytoskeleton (refer to Introduction). Our studies indicate that SH3 contributes to Myo5p
function through control of Myo5p localization. In budding yeast and in other organisms, classic myosin I proteins are resolved in patch-like structures, consistent with
the assumption that these proteins are associated with
some macromolecular complex. These myosin I-containing patches are localized at the cell cortex and are enriched at sites of actin organization and/or polarization.
Here, we show that deletion of the SH3 domain of Myo5p
produces defects in Myo5p localization. Wild-type and
SH3-deleted forms of Myo5p are resolved as punctate structures localized at the cell periphery. Deletion of the
SH3 domain results in depolarization of Myo5p patches.
Thus, the SH3 domain of Myo5p is not required for
Myo5p patch assembly. However, our findings indicate
that the SH3 domain contributes to enrichment of Myo5p
at sites of polarized cell surface growth. This observation is
consistent with previous findings that a classic myosin I of Acanthamoeba, myosin IC, contains an SH3 domain and
colocalizes with the proline-rich myosin IC-binding protein Acan125 (Xu et al., 1995). Since Myo5p patches do
not colocalize with the actin cytoskeleton, our findings
support a role for the SH3 domain in targeting events
which are not strictly directed to the cytoskeleton.
Previous studies indicate that proteins which act early in
the polarity establishment pathway (Vrp1p, Bem1p, and
Cdc42p) and proteins which act during cytokinesis (septin
proteins Cdc10p and Cdc11p) do not require actin for localization. In contrast, actin-binding proteins (Abp1p and
cofilin), and secretory proteins known to interact with actin (Sec4p and Sec8p) require actin for their localization
(Ayscough et al., 1997; Vaduva et al., 1997
). We find that
Vrp1p, a proline-rich protein implicated in establishment of cell polarity, is a possible Myo5p binding partner. Two-hybrid tests document specific binding between the SH3
domains of both myosin I proteins of yeast and Vrp1p. Indeed, this assay for protein-protein interaction indicates
that the SH3 domains of Myo3p and Myo5p can bind to
multiple sites on Vrp1p. Since mutation of a conserved tryptophan residue conserved in the Myo3p SH3 domains
abolished this interaction (data not shown), two-hybrid interaction between Vrp1p and the myosin I SH3 domain
appears to be specific.
Several findings indicate that Vrp1p has functionally significant interactions with yeast myosin I proteins. First, we
find that Myo5p colocalizes and coimmunoprecipitates
with Vrp1p. Second, deletion of the VRP1 gene results in
phenotypes that are similar to those of myosin I double
deletion mutants: both mutants show actin cytoskeletal depolarization and defects in actin dependent functions including endocytosis, bud site selection, and mitochondrial organization (Vaduva et al., 1997). Third, deletion of
VRP1 results in depolarization of both actin patches
(Vaduva et al., 1997
) and Myo5p patches. These observations support the model that Vrp1p binding to the SH3 domain of myosin I proteins contributes to (a) targeting of
myosin I proteins to sites of polarized cell surface growth,
and (b) myosin I function in control of actin organization.
We propose that Vrp1p functions as a proline-rich scaffold
that binds to multiple myosin I proteins and concentrates these proteins at sites of polarized cell surface growth.
Since multiple proline-rich proteins including Srv2p and
Bni1p are localized at sites of polarized cell surface growth
(Freeman et al., 1996
; Evangelista et al., 1997
) it is possible
that multiple SH3-mediated protein-protein interactions
contribute to myosin I targeting in yeast.
Several findings indicate that the SH3 domain contributes to, but is not sufficient for, Myo5p targeting. First,
pSH3-expressing cells show only partial loss of actin organization and only partial defects in actin dependent processes. That is, deletion of the SH3 domain results in a
decrease in the efficiency of Myo5p function in actin organization. Thus, some fraction of the SH3-deleted Myo5p
must reach the correct site of action. Indeed, we find that patches containing SH3-deleted Myo5p are present but
are not enriched at sites of polarized cell surface growth.
Second, we find that a fusion protein containing of the
SH3 domain of Myo5p shows diffuse cytosolic staining
when expressed in yeast at levels comparable to wild-type,
endogenous Myo5p. Thus, the SH3 domain is sufficient neither for the assembly of Myo5p patches, nor for Myo5p
patch polarization. Together, these observations indicate
that multiple factors in addition to the SH3 domain are required for Myo5p assembly and targeting. Indeed, since
the SH3 domain of myoB, a myosin I protein of Dictyostelium, is not required for myoB targeting (Novak and Titus,
1997
), it is possible that the relative contribution of the
SH3 domain to myosin I targeting may vary among cell
types.
One region that may participate in Myo5p targeting is
its TH1 sequence, a highly basic region found in all myosin
I subclasses. Previous studies indicate that the TH1 domain of myosin proteins can bind to acidic phospholipids
(Adams and Pollard, 1989; Doberstein and Pollard, 1992
).
Moreover, the basic TH1 region of brush border myosin I
protein is sufficient to target myosin molecules to actin-rich apical structures (Footer and Bretscher, 1994
). We
find that deletion of the hyperbasic segment of the Myo5p
TH1 region, or deletion of the motor domain of Myo5p,
impairs protein expression and/or stability (Swayne, T.,
and L. Pon, unpublished results). Therefore, we cannot
draw conclusions regarding the role of the TH1 region in
Myo5p targeting from this line of experiments.
Another region that may participate in Myo5p targeting is the actin-binding motor domain. We find that Myo5p patch assembly requires F-actin. LAT-A treatment results in rapid, quantitative actin depolymerization in yeast and also produces a decrease in total staining intensity of myc-tagged Myo5p in punctate structures and an increase in diffuse, cytosolic staining. Myo5p-containing patches that are detected are cortical, but delocalized and equally distributed in mother and daughter cells. Thus, a drug that causes F-actin depolymerization produces partial defects in Myo5p patch assembly and defects in enrichment of Myo5p patches at sites of polarized cell surface growth. Since myosins are actin-binding proteins, the simplest interpretation of this finding is that Myo5p patch assembly requires association of Myo5p with F-actin, presumably through its high-affinity F-actin binding site in the motor domain. Current studies focus on analysis of the actin-binding activity of the Myo5p motor domain, and the role of this activity in Myo5p targeting.
The data we present supports a model for myosin I localization and function in yeast. In this model, Myo5p is assembled into a protein complex at the cell cortex. Assembly of cortical Myo5p patches does not require either the TH2 domain or the SH3 domain of the Myo5p tail. However, Myo5p patch assembly is at least partially dependent upon F-actin. Cortical Myo5p patches are ultimately transported to and concentrated at sites of polarized cell surface growth where they function in polarization of the actin cytoskeleton. This final targeting event requires the SH3 domain of the Myo5p tail, a region that binds to Vrp1p. We propose that Vrp1p serves as a scaffold to bind and concentrate Myo5p patches within the bud. This is the first example of protein-mediated localization of a myosin I protein. Moreover, since Vrp1p has been implicated in actin polarization, our finding provides (a) additional support for a role of myosin I in actin polarization, and (b) an important link between the cell polarity machinery and a mediator of actin organization.
![]() |
Footnotes |
---|
Received for publication 28 October 1997 and in revised form 23 April 1998.
Address all correspondence to Liza A. Pon, Department of Anatomy and Cell Biology, Columbia University P&S 12-425, 630 West 168th Street, New York, NY 10032. Tel.: (212) 305-1947. Fax: (212) 305-3970. E-mail: lap5{at}columbia.eduWe thank F. Chang and P. Brandt (both from Columbia University, New York), and members of the Pon laboratory for critical evaluation of the manuscript; G. Vaduva and A. Hopper for yeast strains and helpful discussion regarding verprolin; S. Swamy for assistance in immunofluorescence microscopy; K. Brown for expert assistance with the electron microscopy; T. Leeuw for helpful discussion regarding Myo3p localization, and I. Pot for two-hybrid analysis of Myo3p protein-protein interactions.
This work was supported by research grants from the American Cancer Society (RPG-97-163-01-C) and National Institutes of Health (GM45735) to L.A. Pon, a National Institutes of Health Grant (NS16036) to L.A. Greene, a Medical Scientists Training Program Award (5T32 GM07367) to B.L. Anderson, and grants from the Natural Sciences and Engineering Research Council of Canada, and the National Cancer Institute of Canada to C. Boone.
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Abbreviations used in this paper |
---|
aa, amino acids; HA, hemagglutinin; LAT-A, latrunculin-A; RT, room temperature; SH3, src homology domain 3; TH1, tail homology domain 1; TH2, tail homology domain 2; YPD, rich, glucose-based media.
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References |
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