Article |
2 Program in Cell and Molecular Biology, Baylor College of Medicine, Houston, TX 77030
3 Department of Immunology, Baylor College of Medicine, Houston, TX 77030
Address correspondence to J.M. Rosen, Dept. of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030. Tel.: 713-798-6210. Fax: 713-798-8012. E-mail: jrosen{at}faculty.bcm.tmc.edu
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Abstract |
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Key Words: breast cancer; FGFR; inducible dimerization; mammary gland development; angiogenesis
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Introduction |
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The expression of FGF ligands and FGFRs is developmentally regulated in the mammary gland (Coleman-Krnacik and Rosen, 1994; Chodosh et al., 2000), where they are generally expressed during ductal morphogenesis and decrease throughout pregnancy and lactation. Ductal morphogenesis is a highly proliferative stage during mammary gland development, during which the gland invades the stromal fat pad and establishes a branched ductal network (Williams and Daniel, 1983). FGFR1 is mainly expressed during this stage of development (Chodosh et al., 2000). Additionally, expression of a dominant negative FGFR2 in the mammary gland during pregnancy has been shown to inhibit lobuloalveolar development (Jackson et al., 1997). This expression profile suggests that FGF signaling plays an important role in normal mammary gland development.
The observation that mouse mammary tumor virus (MMTV) proviral insertion can activate FGF-3, FGF-4, and FGF-8 and leads to mammary tumorigenesis has revealed that FGF ligands are potent mammary gland mitogens (van Leeuwen and Nusse, 1995). The direct transforming activity of the same FGFs in the mammary gland was subsequently confirmed using transgenic mouse models (Daphna-Iken et al., 1998). Additionally, inappropriate regulation of FGFs and their receptors has been observed in breast cancer cell lines and in 1020% of primary breast tumors (Penault-Llorca et al., 1995; Marsh et al., 1999). These data suggest that aberrant FGF signaling may exert potent transforming capacity in the mammary gland.
In the present study, the effects of FGFR1 signaling have been investigated using a novel, inducible FGFR oligomerization and activation system. This system was first characterized in fibroblasts and mammary epithelial cells, and then in a transgenic mouse model. Inducible iFGFR1 signaling in mammary epithelium resulted in progressively invasive lesions occurring rapidly within 24 wk after iFGFR1 activation. iFGFR1 kinase-induced lesions were examined for cell polarity, hormonal dependence, and invasive properties. This work describes the first application of an inducible dimerization system for studying receptor tyrosine kinase signaling in a transgenic mouse model.
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Results |
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The iFGFR1 and FKBPv constructs were tested in both the fibroblast and mammary epithelial cell lines to determine if they could functionally recapitulate effects mediated by FGF signaling in an AP20187-dependent fashion. One characteristic of NIH3T3 fibroblasts is their dependence on serum growth factors for survival and proliferation. iFGFR1 and FKPBv stably transfected NIH3T3 cells were plated in duplicate plates, grown to subconfluence, placed in serum-free media containing either 30 pM AP20187 or vehicle alone, and then observed for morphological changes over a 72-h time course. When grown in serum-free media in the absence of AP20187, iFGFR1 cells displayed distinct morphological features associated with apoptosis, including membrane blebbing, rounding, and detachment from the surface of the cell culture dish (Fig. 1 E, iFGFR1-; unpublished data). Similar results were obtained with the pBK-neo and FKBPv stably transfected cells in serum-free media treated with and without AP20187 (Fig. 1 E, pBKneo-, pBKneo+; unpublished data). However, iFGFR1 cells treated with AP20187 in the absence of serum remained attached to the cell culture dish, formed distinct multicellular foci and did not display morphological features characteristic of apoptosis (Fig. 1 E, iFGFR1+). A twofold increase in the number of viable cells was observed 48 h after treatment with AP20187 as determined by bioreduction of MTS (Fig. 1 E, iFGFR+). These changes were similar to those observed after treatment of NIH3T3 cells with recombinant FGF-8 under the same serum-free conditions (Fig. 1 F, rFGF8). Thus, treatment of cells stably transfected with iFGFR1 with AP20187 induced a survival response and prevented the contact inhibition that is normally observed in NIH3T3 cells.
Caspase-3 activation was assayed in serum-starved, iFGFR1-transduced NIH3T3 cells in the presence or absence of AP20187 to quantitate the iFGFR1-induced inhibition of apoptosis (Fig. 1 G). Cells were placed in serum-free medium with or without AP20187 for 24 h. Under these conditions, a significant dose-dependent decrease in caspase-3 activity was detected beginning with a dose of 0.5 pM AP20187 (Fig. 1 G, solid line). A maximal decrease was achieved with 30 pM AP20187. No significant decrease in caspase-3 activity was detected using the control FKBPv construct (Fig. 1 G, dashed line). An AP20187-dependent increase in mRNA for the antiapoptotic factor Bcl-xl was also observed (unpublished data). These data suggest that iFGFR1 activation can inhibit caspase-3mediated apoptosis caused by serum withdrawal.
Cell cycle analysis was employed in these two different cell types to determine if the AP20187-induced iFGFR1 signaling pathway also regulates proliferation. Transduced NIH3T3 fibroblasts and HC11 mammary epithelial cells were treated with AP20187 in serum-free media and cellular DNA content was quantitated by propidium iodide staining and FACS (Fig. 1 H). NIH3T3 cells expressing iFGFR1 showed no change in proliferation with or without AP20187. However, HC11 cells expressing iFGFR1 showed greater than a twofold increase in proliferation in response to AP20187. In the presence or absence of AP20187, no difference in proliferation was detected in the control FKBPv transduced HC11 or NIH3T3 cells (Fig. 1 H, black bars). These data suggest that iFGFR1-regulated signaling pathways may exert functionally different effects that are cell type dependent. Although iFGFR1 appears to activate the same downstream pathways in fibroblasts and mammary epithelial cells, effects on their proliferation are not equivalent.
Generation of MMTV iFGFR1 transgenic mice
Transgenic mice were generated that express the iFGFR1 construct under the control of the MMTV long-terminal repeat (LTR). iFGFR1 and iFGFR2 constructs were subcloned into an MMTV transgene cassette for the generation of transgenic mice (Leder et al., 1986). Three iFGFR1 and four FGFR2-FKBPv lines were generated that transmitted the transgene through the germline. Of these seven lines, three (one iFGFR1 and two iFGFR2) expressed the transgene in the mammary gland as detected by Western blot analysis of the HA epitope tag (unpublished data). All three expressing lines displayed normal morphology in virgin mice and similar inducible phenotypes described below. A detailed characterization was performed on the iFGFR1 line 4775, which expressed low levels of iFGFR1 mRNA, detectable by RT-PCR, and low levels of protein expression as determined by Western blot analysis (unpublished data).
Activation of iFGFR1 results in increased lateral buds
To analyze the ductal morphology of mammary glands in the expressing transgenic lines, whole-mount analysis of the thoracic and inguinal glands was performed. 6-wk-old transgenic and control mice were treated intraperitoneally with injections (50 µg at 0.5 mg ml-1) of AP20187 every 3 d for 16 d, or by intrascapular implantation of Alzet minipumps containing 100 µl of 0.5 mg ml-1 AP20187 which delivered 0.25 µl h -1 for 2 wk. After AP20187 treatment, the inguinal mammary glands were biopsied for whole-mount and immunofluorescence analyses (Fig. 2). Whole-mount analysis of the mammary epithelium showed no gross morphological differences between age-matched nontransgenic littermates treated with AP20187 and transgenic littermates treated with solvent only (Fig. 2, AD). However, transgenic mice treated with AP20187 displayed increased lateral budding along the ductal epithelium (Fig. 2, E and F). AP20187-induced lateral budding was observed throughout the mammary gland and appeared on primary, secondary, and tertiary ductal branches. Increased lateral budding was also observed at the distal tips of the mammary epithelium (Fig. 2, E and F, arrowhead). Additionally, regional increases in ductal branching were observed in AP20187 treated transgenic mice (Fig. 2, E and F, arrows). Histological analysis revealed extensive lateral buds, with single and multicell-layered epithelium lining the ducts (Fig. 2 L). Although these lateral buds did contain lumens, many of these appeared constricted or convoluted.
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Transgene expression is localized to ductal and lateral bud epithelium
The localization of transgene expression in the mammary gland of transgenic mice was determined by immunofluorescent detection of the HA-epitope on the iFGFR1 protein. Mammary epithelial cells expressing the HA-epitope were observed in transgenic mice, but not in nontransgenic littermates (Fig. 2, M and N). Expression of the iFGFR1 protein was localized to the periphery of the cell and no nuclear staining was apparent, consistent with membrane targeting by the myristylation sequence. Transgene expression was punctate along the ductal epithelium of untreated 8-wk-old transgenic mice (unpublished data), most likely due to an incomplete penetrance of the MMTV LTR-driven transgene, whereas AP20187-induced lateral buds were strongly immunoreactive (Fig. 2 N). Thus, it is possible that AP20187 treatment results in the expansion of transgene positive cells, as a result changing the expression from a punctate to a multifocal pattern. However, not all cells in lateral buds expressed the HA-tagged transgene. Instead, lateral buds contained a mixture of cell types including either transgene positive or progesterone receptor (PR)-positive cells (unpublished data). PR-positive cells within lateral buds and lining the ducts were 5-bromo-2'-deoxyuridine (BrdU) negative, consistent with the observed quiescent nature of PR-expressing cells in the mammary gland (unpublished data) (Seagroves et al., 2000).
Chronic activation of iFGFR1 results in progressively invasive lesions
Whole-mount, histological, and immunofluorescent analyses were utilized to characterize the progressive nature of the iFGFR1-induced lesions. To characterize these lesions morphologically, whole mounts and paraffin-embedded sections were stained with hematoxylin or hematoxylin and eosin (H&E), respectively. In addition, cell polarity was determined by indirect immunofluorescence microscopy using antibodies directed against specific, apical tight junction (ZO-1) and basal, basement membrane (laminin) markers. iFGFR1-induced lesions were classified into three morphologically distinct types. Type I lateral buds were characterized by the presence of a single layer of cubodial epithelium surrounded by myoepithelial cells, and with basally localized laminin and apically localized ZO-1. Type-I lesions appeared within 3 d of AP20187 treatment and were similar to lateral alveolar buds induced in early pregnancy (Fig. 3 A, type I) (Daniel and Silberstein, 1987). However, unlike lobuloalveolar development during late pregnancy, the iFGFR1-induced lateral buds remained closely associated with their primary ducts and did not completely occupy the interductal stroma.
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Type III lesions were first observed after 4 wk of AP20187 treatment and appeared as multifocal lesions along ducts (Fig. 3 A, type III, whole mount). Type III lesions exhibited a marked loss of laminin and ZO-1 expression, and were multicellular, invasive, and highly vascularized (Fig. 3 A, type III, H&E and immunofluorescence). These lesions were often associated with a surrounding leukocyte infiltration and reactive stroma (Fig. 3 A, type III, H&E). Interestingly, analysis of the more invasive type III lesions by FACS suggests that these lesions were predominantly diploid (unpublished data). Thus, type III lesions have some characteristics similar to intraepithelial neoplasia and thus may represent a preneoplasiac growth.
iFGFR1 dimerization induces proliferation and activation of signaling pathways in vivo
Dimerization-induced phosphorylation of the iFGFR1 protein and downstream signaling pathways were analyzed by Western blotting and immunofluorescence in transgenic mice 24 h after the completion of a 2-wk treatment with AP20187. Protein extracts isolated from AP20187-treated and -untreated transgenic littermates showed increased iFGFR1, Akt, and MAPK phosphorylation only in treated mice (Fig. 4, A and B). In transgenic mice treated for 16 d, immunolocalization of the phosphorylated (Thr202/Tyr204) MAPK correlated with the lateral bud lesions (Fig. 4, C and D). These results suggest that AP20187 treatment induced the phosphorylation and activation of the iFGFR signaling cascade in transgenic mice similar to that observed in NIH3T3 and HC11 cells.
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Transgenic mice were treated for 3 d with AP20187, followed by 120 h of drug withdrawal to assess the reversibility of the system. The half-life of AP20187 in the bloodstream has been determined previously to be 7 h (Tim Clackson, ARIAD Pharmaceuticals, personal communication). Both the increase in MAPK phosphorylation and proliferation in the mammary epithelium were not reversible after injections of AP20187 were stopped for 120 h before tissue biopsy (Fig. 4, G and H). Similar results were obtained when mice were treated with AP20187 for 6 wk followed by a withdrawal interval of 2 wk before to tissue biopsy (unpublished data).
iFGFR1 activation alters mammary epithelial cell polarity
Mammary epithelial cells undergo an intrinsic apoptotic response upon detachment from the basement membrane, a process termed anoikis. Confocal microscopy was utilized to determine if the iFGFR1-induced lesions contain cellular layers detached from the basement membrane. Thick frozen sections of mammary glands isolated from AP20187-treated transgenic mice were stained with antiE-cadherin (Texas red) and laminin (FITC) antibodies (Fig. 4, I and J). Wild-type midpregnant mice were used as a control to contrast alveolar buds with iFGFR1-induced lesions. Confocal microscopic analysis showed that the mammary epithelium forms a single layer of cells contacting both the laminin-rich basement membrane (Fig. 4 I, arrowhead) and lumenal space (Fig. 4 I, asterisk) in wild-type midpregnant mice. Additionally, E-cadherin localization was observed only on lateral sides of epithelial cells, at sites of cell-to-cell contact, and not at the apical or basal membranes (Fig. 4 I, arrow). Transgenic mice treated with AP20187 formed multicellular layers with inner (lumenal) layers detached from the basement membrane (Fig. 4 J, arrow). Additionally, E-cadherin staining was observed on the entire periphery of epithelial cells, suggesting cell-to-cell contact was no longer restricted to lateral membranes (Fig. 4 J, arrow). The loss of attachment to the basement membrane and peripheral contacts with neighboring cells suggests that iFGFR1-induced lesions are no longer responsive to polarity signals, including anoikis. This is consistent with iFGFR1 signaling inhibiting apoptosis and inducing proliferation in the mammary epithelium.
iFGFR1-induced lesions have invasive characteristics
The presence of myoepithelial cells, extracellular matrix (ECM) and the vascular network were analyzed in AP20187-treated transgenic mice to assess the invasive characteristics of iFGFR1-induced lesions. The matrix metalloproteinases (MMPs) are a family of related Zn++ interacting proteases that are increasingly implicated in tumorigenesis (Benaud et al., 1998; John and Tuszynski, 2001). Analysis of MMP activity in conditioned media from iFGFR1-expressing mammary epithelial cells was used to determine if MMPs are regulated through iFGFR1 signaling. Using gelatin zymography, medium from serum-starved iFGFR1-expressing HC11 mammary epithelial cells treated with AP20187 showed increased MMP activity over solvent treated cells (Fig. 5 A). Gelatinase activity was inhibited when the chelating reagent EDTA was added to the incubation buffer (Fig. 5 A). Additionally, increased gel migration of MMP-9 was observed after pretreatment of the protein extracts with p-aminophenylmercuric acetate (APMA), an inducer of autocatalytic cleavage of proMMPs (Fig. 5 A). These data demonstrate that iFGFR1 activation in mammary epithelial cells can induce MMP-2 and MMP-9 activity.
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Proliferation of the epithelium in the mammary gland requires concomitant changes not only in the underlying stroma, but also in the vascular network supporting the new growth. The vascular network surrounding iFGFR1-induced lateral buds was observed in situ using confocal microscopy and FITC-lectin visualization of blood vessels. To visualize blood vessels, 2-wk AP20187-treated transgenic mice were injected into the left ventricle with FITC-Lycopersicon esculentum lectin. FITC-lectininjected mice were perfused with fixative, and mammary glands were biopsied and frozen in OCT compound. Mammary glands were cryosectioned and stained with Texas red phalloidin to identify the mammary epithelium. Confocal imaging and software-rendered three-dimensional reconstitution revealed a highly branched network of blood vessels surrounding iFGFR1-induced lateral buds (Fig. 5, F and G). Lateral buds were primarily associated with small tortuous vessels branching off of larger vessels lining the ductal epithelium (Fig. 5, F and G, arrows). These data suggest that sprouting angiogenesis from the existing ductal vascular network may be initiated indirectly by iFGFR1 signaling in the mammary epithelium.
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Discussion |
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The rapid 4-wk time period from the appearance of initial type I to the invasive type III lesions suggests that iFGFR1 signaling in mammary epithelium exerts both potent proliferative and antiapoptotic effects (Fig. 6 A). However, the complex nature of iFGFR1-induced lesions, including the loss of myoepithelium and increased vascular branching, suggest that other indirect effects mediated through stromal interactions also contribute to the invasive characteristics. The conversion of a single layer ductal epithelium to multicell layers found in type II and type III lesions suggest that iFGFR kinase activity inhibited the apoptosis that is normally associated with detachment of lumenal epithelial cells from the basement membrane. In iFGFR1-expressing mice treated with AP20187, no significant changes in apoptosis were observed by TUNEL staining when compared with untreated mice (unpublished data). Levels of apoptosis in the ductal epithelium of mature virgin mice detected by the TUNEL assay are extremely low (<0.1%), thus limiting the ability to detect a decrease in apoptosis (Humphreys et al., 1996; Rosfjord and Dickson, 1999). Interestingly, recent studies using an MMTV LTR-driven Tet-regulatable system have shown that after c-myc activation, there is an increase in both proliferation and apoptosis, reaching levels of 10% in the ductal epithelial cells (D'Cruz et al., 2001). Increased lateral budding and mammary hyperplasia were also observed in this transgenic model, but required a long latency during which other stochastic events, such as Kras2 activation may occur (D'Cruz et al., 2001). In contrast, iFGFR signaling can directly activate both proliferation and survival signals within mammary epithelium to rapidly induce hyperplastic lesions. Moreover, Muthuswamy et al. (1999)(2001), using the same inducible dimerization system, demonstrated that activation of ErbB2 but not ErbB1 in human mammary epithelial MCF10A cells resulted in similar changes in proliferation and a loss of cell polarity demonstrating that highly homologous receptor tyrosine kinases can induce differential effects (Muthuswamy et al., 1999, 2001). The FGF and EGF growth factor receptor families share several downstream signaling factors that may explain the similarity in the iFGFR1- and ErbB2-induced effects on mammary epithelial cells.
During branching morphogenesis of the Drosophila trachea and mouse lung, signaling between the epithelium and mesenchyme is essential in ductal bifurcation, and FGF ligands and FGFRs have been implicated as critical factors in this process (Metzger and Krasnow, 1999; Sekine et al., 1999). The effects of iFGFR signaling described in this study, and the pattern of FGF ligand and FGFR expression during ductal morphogenesis, are consistent with a possible role for this growth factor family in initiating branching in the mammary gland (Fig. 6 B). In this study, iFGFR1 kinase activation elicited direct effects on mammary epithelium including regulation of proliferation, apoptosis, and MMP secretion. Additional indirect effects, including ECM remodeling and vascular branching, may function in restructuring the stroma to provide support for the developing ductal network (Fig. 6 B). Factors initiating branching morphogenesis and lateral budding should induce similar effects during mammary gland development. Therefore, it is likely that coordinated FGF signaling during ductal morphogenesis contributes to this developmental process. Conditional FGFR knockouts will be important to further substantiate the importance of FGF function during mammary gland development.
Because transgene expression levels may contribute to AP20187-independent dimerization of the iFGFR constructs, the line used in these studies (4775) had the lowest levels of protein expression, as detected by Western blot analysis, and required RT-PCR analysis for mRNA detection (unpublished data). However, all expressing lines demonstrated AP20187-independent tumorigenesis in parous mice appearing 612 mo after parturition (unpublished data). It is unlikely that integration effects contribute to this phenotype, as all lines exhibit both normal mammary gland morphology in untreated virgin transgenic mice, and tumors after breeding. It is more likely that during pregnancy and lactation, transgene expression is upregulated to levels that favor AP20187-independent activation, resulting in preneoplastic lesions and leading to tumorigenesis. This is consistent with the appearance of multifocal intraepithelial neoplastic lesions in the mammary glands of untreated parous mice and the long latency for tumor development (unpublished data). However, we cannot rule out other initiating factors contributed by pregnancy, lactation, or involution that may cooperate with iFGFR1 signaling to induce tumorigenesis. The use of the MMTV-LTR promoter to drive expression of the transgene may also contribute to the lack of reversibility of the lesions through the initiation of an autoregulatory loop established upon activation of iFGFR signaling. This is consistent with the observed increase in transgene expression after only 3 d of AP20187 treatment as detected by Western blot analyses (unpublished data). Transgenic mice containing iFGFR1 regulated by a tetracycline dependent promoter crossed with mice containing the reverse tetracycline-dependent transcriptional activator under the control of the MMTV promoter (D'Cruz et al., 2001), may provide an improved model to regulate both expression levels of iFGFR1 using doxycycline and iFGFR1 activity using AP20187 resulting in a reversible system in which to study tumor progression. However, the iFGFR constructs described in our study have been shown to elicit reversible effects when expressed downstream of different promoters in transgenic and tumor cell transplantation models (unpublished data).
The iFGFR1 transgenic mice provide a mouse model for the analysis of progression from early hyperproliferative to invasive lesions in the mammary gland. These mice will be useful in defining the steps necessary for transformation. However, these data must be interpreted with the caveat that the iFGFR transgene contains several differences from the endogenous FGFR1 that may alter its activation kinetics and cellular localization. The iFGFR constructs contain only the intracellular domain of the FGFR and eliminate regulatory elements found in the extracellular and transmembrane domains (Rousseau et al., 1996; Burke et al., 1998; Wang et al., 1999). The extracellular domain of the endogenous receptor confers affinity for ligand and heparin-sulfate proteoglycans that regulate signal intensity, duration, and affect cellular response. The iFGFR constructs contain a Src myristylation sequence to anchor the receptor to the plasma membrane. Thus, it is likely that this may influence the localization of the iFGFR, and also, potentially, the duration and nature of iFGFR signaling. The examination of signaling molecules that are activated by differentially localized constructs should reveal whether basolateral versus nonlocalized expression affect the duration and specificity of RTK-induced signaling pathways. Therefore, further comparison between iFGFR and FGFR signaling will be required to assess the capacity of the AP20187/FKBPv system to completely recapitulate the endogenous signaling pathways.
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Materials and methods |
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Survival and proliferation assay
For cell survival assays, the pSH1-iFGFR1 construct was cotransfected with pBKneo into NIH3T3 cells using Fugene. Stable cells were selected by supplementing growth media with 200 µg of active G418 ml-1 for 3 wk. Pooled NIH3T3 cells were placed in serum-free DME media for 72 h with daily media changes for morphological analysis. Images were captured using a Sony CCD-DXC-151A camera. Caspase-3 activity was measured by cleavage of the fluorogenic substrate Ac-DEVD-AMC (PharMingen). Briefly, iFGFR1 and FKBPv-transduced NIH3T3 cells were treated with AP20187 or ethanol diluent in serum-free media for 24 h, followed by cell lysis and incubation with the fluorogenic peptide according to the manufacturer's protocol. Cell proliferation was measured, following the manufacturer's protocol, by propidium iodide (Roche) staining of AP20187 or ethanol diluenttreated cells in serum-free media for 48 h. DNA content was measured by FACS. For in vivo proliferation, a BrdU label retention assay was performed as previously described by Seagroves et al. (1998). Assays were performed in triplicate or quadruplicate and repeated at least two times. BrdU quantitation was performed by counting positive cells from at least five 40x images from two or more mice.
Western blots and zymography
The following antibodies were used for Western blot analysis: phospho-MAPK; phospho-Akt and Akt (Cell Signaling); PY20 (BD Transduction Laboratories); FRS2 (Santa Cruz Biotechnology); and HA-epitope (Santa Cruz Biotechnology). Protein extracts from cultured cells were isolated with Triton X-100 extraction buffer (150 mM NaCl, 1% Triton X-100, 1 µg ml-1 aprotinin, 1 µg ml-1 benzeamidine, 1 µg ml-1 antipain, 10 µg ml-1 soybean trypsin inhibitor, 0.25 µg ml-1 leupeptin, 100 µM PMSF, 100 uM NaVanadate, 1 mM NaF) and passed through a 26-G syringe five times followed by incubation on ice for 45 min. Protein extracts were quantitated by Bradford assay (Bio-Rad), and 80 µg protein was resolved by SDS-PAGE and transferred onto PVDF membranes (Millipore). Western blot analyses were followed as previously described (Seagroves et al., 1998). Chemiluminsecence was developed following manufacturer's protocol (Pierce Chemical Co.). For zymography, 1.5 ml media from AP20187 or ethanol diluenttreated HC11 cells in serum-free media for 16 h was concentrated with YM-10MW Centricon membranes following the manufacturer's protocol (Millipore), loaded in equal volumes on gelatin zymography gels, and analyzed as described by Talhouk et al. (1991).
Indirect immunofluorescence and histology
The following primary antibodies were used for indirect immunofluorescent detection of specific antigens: rabbit polyclonals were antikeratin-14 (Convance Research Products); anti-laminin (Dako); and antiphospho-MAPK (Cell Signaling). Rat monoclonals were antiE-cadherin (Zymed) and antiZO-1 (Chemicon), and mouse monoclonals were antiHA-epitope (Convance Research Products) and anti-BrdU (Becton Dickinson). Secondary antibodies were: antimouse Texas red; Texas red antirat; FITC antirabbit; and Texas red antirabbit (Molecular Probes). Tissue was processed and stained as previously described (Seagroves et al., 1998). For confocal microscopic analysis of frozen sections, tissue was fixed in 4% PFA/PBS for 2 h and frozen in OCT. Frozen sections were cut (50 µm) and stained overnight with primary antibody in 3%BSA/PBS. Sections were washed in PBS for 10 min and stained with secondary antibodies for 5 h. Sections were then washed for 30 min, mounted with Vectashield, and analyzed using a Zeiss 510 laser scanning confocal microscope. Whole mounts were prepared as previously described (Williams and Daniel, 1983), and images were captured using Olympus dissecting microscope and Sony video camera (#DXC-151A). H&E sections were prepared as previously described (Seagroves et al., 1998). Masson's trichrome staining was performed with Accustain Trichrome Stain following the manufacturer's protocol (Sigma-Aldrich).
FITC-lectin perfusion
Mice were implanted with Alzet minipumps (Alzet) containing 100 µl of 0.5 mg ml-1 AP20187, delivering 0.25 µl h-1 for 2 wk. Mice were anesthetized after 2 wk with Avertin followed by left ventricular injection of Lycopersicon esculentum FITC-lectin (Vector). After 5 min, 5 ml of 1% paraformaldehyde/0.5% gluteraldehyde/PBS was perfused followed by 5 ml of PBS. Tissue was then dissected and frozen in OCT. For confocal microscopy, thick 50-µm sections of OCT embedded tissue were cut and postfixed in 4% PFA/PBS for 10 min at room temperature. Sections were then permeablized with 1% Triton X-100 in PBS for 10 min at room temperature, washed in PBS for 5 min, and stained for 5 h with phalloidin-Alexa594 (Molecular Probes). Sections were washed for 20 min in PBS, mounted with Vectashield, and imaged.
Online supplemental material
Supplemental videos are available at http://www.jcb.org/cgi/content/full/200107119/DC1. Video 1 shows a midpregnant mouse mammary gland consisting of a single cell layer of polarized mammary epithelium. Three-dimensional rotation of the midpregnant mammary gland demonstrates the single-cell epithelial layer between the basement membrane and lumen. Video 2 represents iFGFR1-induced lesions containing multicell-layered mammary epithelium with altered cell polarity. Confocal imaging through the iFGFR1-induced lesion demonstrates the multiple cell layers, reduced lumen size, and peripheral staining of E-cadherin observed throughout these lesions. Videos 3 and 4 show increased vascular branching surrounding iFGFR1-induced lateral buds. Confocal immunofluorescence and three-dimensional imaging of the vascular network shows the close proximity and branching of blood vessels surrounding iFGFR1-induced lateral buds.
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Footnotes |
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B.E. Welm's present address is Dept. of Anatomy, University of California, San Francisco, San Francisco, CA 94143.
* Abbreviations used in this paper: BrdU, 5-bromo-2'-deoxyuridine; ECM, extracellular matrix; FGF, fibroblast growth factor; FGFR, FGF receptor; H&E, hematoxylin and eosin; HA, hemagglutinin; LTR, long terminal repeat; MAPK, mitogen-activated protein kinase; MMP, matrix metalloproteinase; MMTV, mouse mammary tumor virus; PR, progesterone receptor; TEB, terminal-end bud; TUNEL, TdT-mediated dUTP-biotin nick end labeling.
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Acknowledgments |
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B. Welm was supported by a predoctoral fellowship from the Department of Defense Breast Cancer Research Program (DAMD17-98-1-8283). This research was supported by grant CA16303 from the National Cancer Institute.
Submitted: 27 July 2001
Revised: 22 March 2002
Accepted: 22 March 2002
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