Correspondence to: Jochen H.M. Prehn, Interdisciplinary Center for Clinical Research (IZKF), Research Group “Apoptosis and Cell Death,” Faculty of Medicine, Westphalian Wilhelms-University, Röntgenstrasse 21, D-48149 Münster, Germany. Tel:49-251-83-52251 Fax:49-251-83-52250 E-mail:prehn{at}uni-muenster.de.
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Abstract |
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NGF has been shown to support neuron survival by activating the transcription factor nuclear factor-B (NF
B). We investigated the effect of NGF on the expression of Bcl-xL, an antiapoptotic Bcl-2 family protein. Treatment of rat pheochromocytoma PC12 cells, human neuroblastoma SH-SY5Y cells, or primary rat hippocampal neurons with NGF (0.110 ng/ml) increased the expression of bcl-xL mRNA and protein. Reporter gene analysis revealed a significant increase in NF
B activity after treatment with NGF that was associated with increased nuclear translocation of the active NF
B p65 subunit. NGF-induced NF
B activity and Bcl-xL expression were inhibited in cells overexpressing the NF
B inhibitor, I
B
. Unlike tumor necrosis factor-
(TNF-
), however, NGF-induced NF
B activation occurred without significant degradation of I
Bs determined by Western blot analysis and time-lapse imaging of neurons expressing green fluorescent proteintagged I
B
. Moreover, in contrast to TNF-
, NGF failed to phosphorylate I
B
at serine residue 32, but instead caused significant tyrosine phosphorylation. Overexpression of a Y42F mutant of I
B
potently suppressed NFG-, but not TNF-
induced NF
B activation. Conversely, overexpression of a dominant negative mutant of TNF receptor-associated factor-6 blocked TNF-
, but not NGF-induced NF
B activation. We conclude that NGF and TNF-
induce different signaling pathways in neurons to activate NF
B and bcl-x gene expression.
Key Words:
nerve growth factor, nuclear factor-B, Bcl-xL, tumor necrosis factor-
, I
B
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Introduction |
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Bcl-xL is an antiapoptotic Bcl-2 family protein that is widely expressed in the developing and adult nervous system (
Little is known about the regulation of bcl-x gene expression in the nervous system. In blood cells, transcription of the bcl-x gene is controlled by transcription factors, signal transducer, and activator of transcription 5 and nuclear factor B (NF
B)1 (
B subunits p65/relA and c-rel have been demonstrated by functional analysis of the bcl-x promoter (
activate NF
B by inducing the degradation of I
B proteins. These are cytosolic proteins associated with NF
B subunits that function as their inhibitors (
B proteins has been shown to involve phosphorylation at serine residues, ubiquitination, and subsequent degradation via the 26S proteasome complex (
We have previously shown that the cytokine transforming growth factor-ß1 also regulates the expression of the antiapoptotic proteins Bcl-xL and Bcl-2 in primary neuron cultures ( has recently been shown to increase Bcl-xL expression in neurons in an NF
B-dependent manner (
B activation is not only involved in the nervous system response to injury or inflammation, but is also required to support neuron survival during development and in the adult nervous system. Activation of excitatory amino acid receptors (
B activity in neurons (
B activity in various neuronal and nonneuronal populations (
B-dependent pathway. Moreover, we demonstrate that NGF-induced NF
B activation requires tyrosine phosphorylation of the inhibitor I
B
, but occurs independently of serine phosphorylation and degradation of I
Bs via the proteasome.
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Materials and Methods |
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Materials
Murine 2.5S NGF and recombinant human TNF- were from Promega. The proteasome inhibitors carbobenzoxyl-leucinyl-leucinyl-leucinal (MG132) and lactacystin were purchased from Biomol. Sodium pervanadate (Sigma-Aldrich) was prepared as described by
Cell Culture
Rat pheochromocytoma PC12 cells were grown in DME medium (Life Technologies) supplemented with 10% horse serum (PAN Biotech), 5% FCS (PAA) and the antibiotic mixture of 100 U/ml penicillin and 100 µg/ml streptomycin (Life Technologies). Human neuroblastoma SH-SY5Y cells were grown in RPMI 1640 medium (Life Technologies) supplemented with 10% FCS and the antibiotic mixture. Hippocampal neurons were prepared from neonatal (P1) 344 rats (Fisher Scientific) as described (
Reverse Transcription PCR
Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). Purity of samples and RNA content were measured using a UV photometer (Amersham Pharmacia Biotech). 1 µg of total RNA was reverse-transcribed and amplified in a single reaction tube in a volume of 50 µl. The reverse transcription (RT) reaction was performed at 42°C for 20 min in the presence of oligo(dN) primers and Moloney murine leukemia virus reverse transcriptase (Amersham Pharmacia Biotech). After heat inactivation, specific oligonucleotide primer pairs for bcl-x and GAPDH (20 pmol each; MWG) were added to the reaction mixture (1.5 mM MgCl2, 60 mM KCl, 10 mM Tris-HCl, pH 9.0, 200 µM deoxynucleotides each and 2 U Taq polymerase; Amersham Pharmacia Biotech). Primer pairs were based on Mus musculus bcl-x and Rattus norvegicus GAPDH sequences. The sequences of the primers were as follows: bcl-x sense primer, 5'-GGA GAG CGT TCA GTG ATC-3' and bcl-x antisense primer, 5'-CAA TGG TGG CTG AAG AGA-3'; GAPDH sense primer, 5'-CTC GTG GTT CAC ACC CAT-3' and GAPDH antisense primer, 5'-GGC TGC CTT CTC TTG TGA-3'. PCR was performed for 22 (bcl-x) or 15 (GAPDH) cycles at 94°C for 30 s, 58°C for 60 s, and 72°C for 120 s using a Primus 25 thermocycler (MWG). The amplified PCR products (expected size for bcl-xL: 472 bp; expected size for GAPDH: 355 bp) were separated on a 5% agarose gel containing 0.1% ethidium bromide and visualized using a CCD camera-based documentation system (MWG). Intensity of bands was analyzed using ONEDscan software (Scanalytics). The intensity of the GAPDH amplification product served as internal control. Amplification temperature and cycle number with respect to the linear range of the amplification process were empirically determined for each primer pair.
SDS-PAGE and Western Blotting
Cultures were rinsed with ice-cold PBS and lysed in TBS containing SDS, glycerin, and protease inhibitors. Protein content was determined using the BCA Micro Protein Assay kit (Pierce Chemical Co.) and samples were supplemented with 2-mercaptoethanol and denaturated at 95°C for 5 min. An equal amount of protein (2050 µg) was separated by SDS-PAGE and blotted to nitrocellulose membranes (Protean BA 85; Schleicher & Schuell). Equal loading of samples was confirmed by Ponceau red staining. Nonspecific binding was blocked at room temperature for 2 h by incubation in TBS containing 0.1% Tween-20, BSA, and nonfat dry milk. The blots were then incubated over night at 4°C with the primary antibodies diluted in blocking buffer. Antibodies used were a rabbit polyclonal antiBcl-x antibody (a gift from Prof. Craig B. Thompson, University of Pennsylvania, Philadelphia, PA) diluted 1:5,000, a mouse monoclonal anticyclooxygenase-2 (COX-2) antibody (clone 33; Transduction Laboratories) diluted 1:2,000, a rabbit polyclonal antibody specific for serine 32-phosphorylated IB
(P-Ser32-I
B
) (New England Biolabs, Inc.) diluted 1:2,000, rabbit polyclonal antibodies recognizing I
B
(New England Biolabs, Inc., and sc-203; Santa Cruz Biotechnology, Inc.) diluted 1:2,000, a rabbit polyclonal antibody specific for I
Bß (sc-945; Santa Cruz Biotechnology, Inc.) diluted 1:1,000, and a mouse monoclonal anti
-tubulin antibody (clone DM 1a; Sigma-Aldrich) diluted 1:2,000. Afterwards, membranes were washed and incubated with antimouse or rabbit IgG-HRP conjugate (1:5,000; Promega). Antibody-conjugated peroxidase activity was visualized using the Super Signal chemiluminescence reagent (Pierce Chemical Co.). Immunoblots were stripped in stripping buffer (2% SDS, 62.5 mM Tris-HCl, 100 mM 2-mercaptoethanol, pH 6.8) for 20 min at 60°C, washed, and reprobed.
Immunocytochemistry and Visualization of the Active p65 NFB Subunit
After stimulation with NGF, cells were washed and fixed with 4% paraformaldehyde in PBS at 37°C for 20 min. Fixed cells were permeabilized by treatment with ice-cold 0.1% Triton X-100 in PBS. Nonspecific antibody binding was blocked by PBS, pH 7.4, containing 2% nonfat dry milk, 2% BSA, and 0.1% Tween 20 for 1 h at room temperature. The active p65 NFB subunit was visualized using a mouse monoclonal antibody (clone 12H11; Roche) that recognizes an epitope overlapping the nuclear location signal of the p65 NF
B subunit (
Analysis of NFB Reporter Gene Activity
PC12 cells were seeded on poly-L-lysine coated 24-well plates at a density of 104 cells per well. Cells were then transfected with 0.75 µg of a plasmid containing four tandem repeats of the enhancer element fused to the herpes simplex virus thymidine kinase promoter upstream of the coding sequence for a secreted form of human placental alkaline phosphatase (SEAP) (pNF
B-SEAP; CLONTECH Laboratories, Inc.). In the experiment shown in Fig 3 (below), PC12 cultures were cotransfected with 0.25 µg of a human wild-type I
B
expression plasmid (pI
B
) controlled by the cytomegalovirus promoter or control DNA of similar kilobase size. The I
B
expression plasmid was originally generated and published by
B
or mutant I
B
Y42F pcDNA expression plasmid (
, or vehicle was added. The medium was collected after 6 and 24 h and a fluorescence SEAP assay was performed (Great EscaAPe Fluorescence Detection Kit; CLONTECH Laboratories, Inc.) using 4-methylumbelliferyl phosphate as substrate for SEAP. Fluorescence intensity was measured using a fluorescence plate reader (HTSoft 7000; PerkinElmer) (360 nm excitation, 465 nm emission). Each set of experiments included cultures transfected with a plasmid identical to the reporter construct but lacking the
enhancer element as negative control for background signals. As control for transfection efficiency, cultures were cotransfected with a pSVß-galactosidase plasmid, and NF
B reporter gene activity was normalized to ß-galactosidase expression.
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Overexpression of IB
and Immunofluorescence Analysis
PC12 cells were plated onto poly-L-lysinecoated eight-well tissue culture slides (Becton Dickinson) at a density of 104 cells per well. Cultures were then cotransfected with 0.25 µg of pIB
or control plasmid DNA, as well as a plasmid encoding enhanced green fluorescent protein (EGFP) as transfection control. After 72 h recovery, cells were exposed to NGF, TNF-
, or vehicle for 6 h, fixed, and permeabilized as described above. After blocking for 1 h at room temperature, the specimens were incubated at 4°C overnight with the rabbit polyclonal Bcl-x antibody diluted 1:500 in blocking buffer. After washing, biotin-conjugated antirabbit IgG (1:1,000; Vector Laboratories) was added for 1 h at room temperature, followed by a streptavidinTexas red conjugate (1 µg/ml, 20 min; Molecular Probes). Texas red fluorescence was observed using the Eclipse TE300 inverted microscope and a 40x oil immersion objective (Nikon) with the following optics: 510560 nm excitation, 575 nm dichroic mirror, >590 nm emission. For the observation of the EGFP fluorescence, the following optics were used: 465495 nm excitation, 505 nm dichroic mirror, 515555 nm emission. Digital images of equal exposure were acquired with the SPOT-2 camera using Metamorph software (Universal Imaging Corp.). For quantification of Bcl-xL immunoreactivity, average pixel intensity of the Texas red fluorescence of single cells expressing EGFP was measured using Metamorph software. Background fluorescence intensities of the specimen were substracted from the values.
Immunoprecipitation
After exposure to NGF, sodium pervanadate, or vehicle, PC12 cells were rinsed with PBS and lysed in buffer containing (mM): 50 Tris-HCl, pH 7.5, 0.5% NP-40, 10% glycerol, 250 NaCl, 5 EDTA, 50 NaF, 0.5 Na3VO4, 10 ß-glycerophosphate, and the protease inhibitors PMSF (0.5 mM), leupeptin, and aprotinin (5 µg/ml). Protein content was determined and 250 µg protein extract was immunoprecipitated using a mouse monoclonal antibody (0.5 µg) recognizing tyrosine-phosphorylated proteins (sc-508; Santa Cruz Biotechnology, Inc.). As negative control, lysates were immunoprecipitated using mouse control IgG (Santa Cruz Biotechnology, Inc.). Samples were rotated overnight at 4°C and the antibodyprotein complexes were precipitated for 2 h at 4°C using protein agarose A/G plus (Santa Cruz Biotechnology, Inc.). The beads were centrifuged and washed four times. Samples were supplemented with 2-mercaptoethanol and denatured at 95°C for 2 min. Immunoprecipitated proteins were subjected to 10% SDS-PAGE, blotted, and detected using a rabbit polyclonal antiIB
antibody (sc-203; Santa Cruz Biotechnology, Inc.), diluted 1:2,000. For immunoprecipitation control, the supernatants were collected and aliquots were subjected to SDS-PAGE. I
B
protein was not detectable in the supernatants.
Time-Lapse Imaging of Neurons Expressing IB
-EGFP
PC12 cells were plated at a density of 104 cells on 35-mm glass-bottom dishes (Willco BV) coated with poly-L-lysine. Cultures were then transfected with 0.75 µg of a plasmid encoding an IB
-EGFP fusion protein (pI
B
-EGFP; CLONTECH Laboratories, Inc.) or a plasmid expressing EGFP. After 24-h recovery, EGFP fluorescence was observed using an Eclipse TE 300 inverted microscope and a 40x oil immersion objective equipped with the appropriate filter set (465495 nm excitation, 505 nm dichroic mirror, 515555 nm emission). Time-lapse digital images of equal exposure were acquired with the SPOT-2 camera using Spot software version 2.2.1. After acquiring the first image, cells transfected with pI
B
-EGFP were incubated with NGF, TNF-
, or vehicle directly on the stage. In control experiments, cultures transfected with EGFP were exposed to TNF-
. The incubation medium was enriched with 10 mM Hepes and thoroughly mixed to ensure a proper distribution of the agents. Images were analyzed using Metamorph software. Fluorescence data are given as change in average pixel intensity compared with the first image. Background fluorescence of each image was subtracted from the values.
Statistics
Data are presented as means ± SEM. For statistical comparison, one-way analysis of variance followed by LSD test were employed. Kruskal-Wallis H test followed by Bonferroni-corrected Mann-Whitney U test were used for statistical evaluation of nonparametric data. P < 0.05 was considered to be statistically significant.
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Results |
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Nerve Growth Factor Upregulates Neuronal bcl-xL mRNA and Bcl-xL Protein Expression
To investigate the effect of NGF on neuronal bcl-xL mRNA expression, PC12 cells were treated with 10 ng/ml NGF for time periods of 18 h. Total RNA was isolated and subjected to semiquantitative RT-PCR using bcl-x and GAPDH-specific oligonucleotide primers. bcl-xL mRNA expression increased in the PC12 cells after 2 h of NGF treatment, remained at a high level after 4 h, and then declined after 8 h (Fig 1 a). In agreement with the RT-PCR data, we detected increased Bcl-xL protein expression in PC12 cells treated with NGF (Fig 1 b). Interestingly, a strong response was already observed in cultures treated with NGF concentrations as low as 0.1 and 1 ng/ml. The Bcl-xL level of cultures treated with very high NGF concentrations (50100 ng/ml) declined again (data not shown).
NGF also increased Bcl-xL protein expression in primary rat hippocampal neurons, with maximal effects again seen after treatment with 1 ng/ml NGF (Fig 1 c). bcl-xL mRNA and Bcl-xL protein expression was also increased in human neuroblastoma SH-SY5Y cells treated with NGF (Fig 1 d and data not shown).
Nerve Growth Factor Activates NFB
NFB binding sequences have been identified in the promoter region of the human and murine bcl-x gene (
B activity after treatment with NGF, we performed an immunocytochemical analysis in PC12 and SH-SY5Y cells using a monoclonal antibody raised against an epitope overlapping the nuclear localization signal of the NF
B p65 subunit. This epitope is normally inaccessible because of binding of the endogenous inhibitor I
B (
B subunit p65 in the nucleus with maximal effects observed at a concentration of 1 ng/ml (Fig 2, a and b). Treatment of human SH-SY5Y neuroblastoma cells with NGF also increased the immunoreactivity of the active p65 subunit, both in the cytoplasm and in the nucleus (Fig 2c and Fig d).
To provide direct evidence for increased NFB activity after NGF treatment, PC12 cells were transfected with a reporter plasmid containing four tandem repeats of the
enhancer element fused to the herpes simplex virus thymidine kinase promoter upstream of the coding sequence for the reporter gene SEAP. Treatment of PC12 cells with 1 ng/ml NGF induced NF
B activity to an extent comparable with that induced by TNF-
(10 ng/ml), a cytokine that is known to increase NF
B activity in neurons (
B activity could also be detected by reporter gene analysis in human SH-SY5Y neuroblastoma cells exposed to NGF (data not shown).
Finally, evidence for increased NFB activity after NGF treatment was provided by determining the expression of the NF
B target gene COX-2 (
(Fig 2 f).
NGF-Induced NFB Activity and Bcl-xL Expression Is Inhibited in PC12 Cells Overexpressing I
B
To investigate the requirement of NFB activation for NGF-induced Bcl-xL expression, PC12 cells were transiently transfected with a plasmid encoding the NF
B inhibitor I
B
or control DNA. Overexpression of I
B
reduced both NGF- and TNF-
induced NF
B activity analyzed by the reporter gene assay (Fig 3 a). We next analyzed Bcl-xL protein expression in I
B
-overexpressing PC12 cells cotransfected with EGFP as transfection control. In agreement with our observations described above (Fig 1), Bcl-xL immunofluorescence increased significantly after a 6-h treatment with NGF (1 ng/ml) in cultures transfected with control DNA (Fig 3b and Fig c). Increased Bcl-xL expression was also observed in control-transfected PC12 cells exposed to TNF-
(10 ng/ml). Overexpression of I
B
did not significantly alter the level of Bcl-xL expression in vehicle-treated control cells (ANOVA and LSD test; P = 0.131). Interestingly, however, NGF and TNF-
failed to increase neuronal Bcl-xL expression in cells overexpressing the inhibitor.
TNF-, but Not NGF Induces Rapid Degradation of I
Bs
IB degradation has been shown to be required for activation of NF
B by proinflammatory cytokines (
B
protein degradation in PC12 cells exposed to TNF-
or NGF (Fig 4, a and b). TNF-
induced significant I
B
degradation, starting 10 min after the onset of treatment. In contrast, treatment with NGF for up to 8 h failed to induce significant degradation of I
B
. NGF also failed to trigger the degradation of a second NF
B inhibitory protein, I
Bß (
Bß degradation also occurred in TNF-
treated cultures, albeit with slower kinetics.
To analyze IB
degradation in response to NGF and TNF-
on the single cell level, PC12 cells were transfected with a plasmid encoding EGFP-tagged I
B
. The fusion protein has been previously shown to be degraded after serine phosphorylation with kinetics similar to I
B
, resulting in a decrease in cellular I
B
-EGFP fluorescence (
B
-EGFPtransfected cells with TNF-
induced a significant decrease in fluorescence after 10 min of exposure (Fig 4 c). In contrast, cellular I
B
-EGFP fluorescence did not decrease in cells treated with NGF or vehicle monitored up to 2 h. As a control for specificity of the TNF-
induced decrease in I
B
-EGFP fluorescence, PC12 cells were transfected with a plasmid encoding EGFP. Treatment with TNF-
did not induce a decrease in cellular EGFP fluorescence.
IB
Is Not Phosphorylated at Serine 32 after NGF Stimulation
IB
has been shown to be phosphorylated at serine 32 and 36 residues after treatment with NF
B-inducing cytokines (
B
after treatment with NGF, we performed immunoblot experiments using an antibody specific for P-Ser32-I
B
. While treatment of PC12 cells with TNF-
induced an increase in P-Ser32-I
B
after 10 min of exposure with maximal effects seen after 30 min, NGF failed to induce any significant increase in P-Ser32-I
B
up to 8 h after its addition to the cultures (Fig 5 a). At this time point, NGF had already caused a pronounced increase in NF
B activity (Fig 2). The (lack of) effect was independent of the NGF concentration used, as similar results were obtained in cultures treated with 10 ng/ml NGF (data not shown). TNF-
, but not NGF-induced serine 32 phosphorylation of I
B
was also observed in human SH-SY5Y neuroblastoma cells (Fig 5 b).
Tyrosine Phosphorylation of IB
in NGF-stimulated PC12 Cells
Phosphorylation of IB
at tyrosine residue 42 has been shown to activate NF
B without requiring I
B
degradation via the proteasome (
B
. PC12 cells were exposed to NGF (10 ng/ml) or sodium pervanadate (200 µM) as a positive control, and cytosolic extracts were subjected to immunoprecipitation using a murine monoclonal antibody raised against tyrosine-phosphorylated proteins. As a negative control, cell lysates were immunoprecipitated with mouse control IgG. Detection of immunoprecipitated proteins by SDS-PAGE and Western blot analysis using an I
B
antibody demonstrated that sodium pervanadate induced significant tyrosine phosphorylation of I
B
(Fig 5 c). Treatment with NGF also induced strong tyrosine phosphorylation of I
B
after 120 min of treatment. Tyrosine-phosphorylated I
B
could also be detected in response to TNF-
(data not shown), presumably a consequence of the known ability of NF
B-activating cytokines to stimulate NGF synthesis in neural cells (
To investigate whether phosphorylation of IB
at tyrosine residue 42 mediated NGF-induced NF
B activation, cells were transfected with a plasmid encoding wild-type I
B
or a mutant I
B
(Y42F), which has been shown to block NF
B activation after tyrosine phosphorylation of I
B
(
B
plasmid DNA to a concentration at which NGF and TNF-
were still able to elicit significant NF
B activation (Fig 5 d). Transfection with the same concentration of plasmid DNA encoding mutant Y42F I
B
led to a complete inhibition of NGF-induced NF
B activation. In contrast, TNF-
induced NF
B activation was not inhibited by the I
B
Y42F mutant.
Overexpression of a Dominant-Negative Mutant of TRAF-6 Inhibits TNF--, but Not NGF-induced NF
B Activation
TRAF proteins are proximal signaling components required for TNF-induced NF
B activation (
B via p75 NGF receptors has been shown to involve the association of TRAF6 to the receptor complex (
and NGF upstream of I
B phosphorylation, we transiently expressed TRAF6 dn in PC12 cells (Fig 6). Overexpression of TRAF6 dn potently inhibited TNF-
, but failed to inhibit NGF-induced NF
B activation.
NGF Induces Bcl-xL Expression in the Presence of Proteasome Inhibitors
NFB activation via tyrosine phosphorylation of I
B
occurs independently of I
B
ubiquitination and degradation via the proteasome (
B-target genes independent of the proteasome, we treated PC12 cells with two proteasome inhibitors, MG132 and lactacystin. Treatment with these inhibitors significantly reduced basal Bcl-xL expression in PC12 cells (Fig 7 a). As expected, treatment with NGF was able to induce a strong increase in Bcl-xL expression in the presence of the two inhibitors. Semiquantitative RT-PCR revealed that NGF also increased neuronal bcl-xL mRNA expression in cultures treated with proteasome inhibitors (data not shown). Similarly, NGF increased the expression of the NF
B target gene COX-2 in the presence of the proteasome inhibitor lactacystin (Fig 7 b). In contrast, treatment with lactacystin inhibited TNF-
induced Bcl-xL expression (Fig 7 c).
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Discussion |
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In the present study, we have demonstrated that NGF induced the expression of the antiapoptotic protein Bcl-xL in rat pheochromocytoma PC12 cells, human neuroblastoma SH-SY5Y cells, and primary rat hippocampal neurons. Upregulation of bcl-x expression in response to NGF has previously been observed in PC12 cells (B inhibitor I
B
, indicating that NF
B activation was required for the upregulation of neuronal Bcl-xL expression (
B has also been shown to increase the expression of inhibitor of apoptosis proteins (
TNFs- and -ß, as well as cytokines of the interleukin-6 family have also been shown to increase NF
B activity in cultured neurons (
B activity comparable with that induced by TNF-
. Of note, our data demonstrate that the pathway activated by NGF is distinct from that activated by TNF-
. NF
B activity induced by TNF-
involves serine phosphorylation of I
B proteins via I
B kinases (
B
and degradation of I
B proteins, could be clearly detected in response to TNF-
. In contrast, NGF did not lead to significant serine phosphorylation, but instead induced tyrosine phosphorylation of I
B
. The effect of NGF was mimicked by pervanadate, an agent that activates NF-
B via tyrosine phosphorylation of I
B
at residue 42 (
B
potently suppressed NFG-, but not TNF-
induced NF
B activation. This suggests that (a) tyrosine phosphorylation at this site is required for NGF-induced NF
B activation, and (b) TNF-
induced serine phosphorylation of I
B
is sufficient to activate NF
B.
Phosphorylation of IB
on tyrosine residue 42 has also been observed pathophysiologically in response to hypoxia/reoxygenation (
B
. Tyrosine-phosphorylated I
B
has been reported to have a half life similar to that of nonphosphorylated I
B
, and activation of NF
B may occur by a degradation-independent dissociation of the inhibitor from the p65 subunit (
B
degradation. We cannot fully exclude the absence of I
B
degradation in light of the rapid turnover of I
B
in cultured neurons and the fact that I
B
is rapidly resynthesized as a consequence of NF
B activation (
B activation. NGF activates the PI3-kinase/Akt kinase pathway in neurons (
B activation after tyrosine phosphorylation of I
B
. The authors observed an interaction of the COOH-terminal SH2 domain of p85 with tyrosine phosphorylated I
B
. It remains to be shown whether this interaction induces a dissociation of the I
B
/NF
B complex in the absence of I
B
degradation. However, NGF-induced PI3-kinase/Akt activation may also stimulate additional regulatory steps in NF
B-dependent gene transcription; for example, by increasing the transactivation potential of NF
B (
The rat pheochromocytoma PC12 and the human neuroblastoma SH-SY5Y cells used in the present study express both the trkA and p75 neurotrophin receptors (our unpublished data). Submaximal NGF concentrations (0.11 ng/ml) were sufficient to induce NFB activity and Bcl-xL expression, suggesting that the p75 receptor may play a role in potentiating the effect of NGF transduced via the trkA receptor (
B activity in the absence of trkA receptors (
B activation via selective activation of p75 in the trkA-deficient Schwann cell line RN22 has been shown to involve both serine phosphorylation and degradation of I
B
(
B via a pathway resembling that induced by TNF-
. In support of this concept, the p75 receptor also associates with TRAF proteins (
induced NF
B activation (
induced NF
B activation in PC12 cells was potently inhibited by overexpression of a dominant-negative mutant of TRAF6, while NGF-induced NF
B activation was not suppressed. Because selective activation of NF
B via p75 NGF receptors has been shown to be sensitive to overexpression of dominant-negative TRAF6 (
B activation in PC12 cells primarily involved a signal transduction pathway downstream of trkA receptors. Nevertheless, the extent of serine and tyrosine phosphorylation of I
Bs as well as the extent of I
B degradation via the proteasome in response to NGF may vary in different cell types depending on the relative contribution of the signal transduction pathways activated by p75 and trkA receptors (
There is also evidence for a cross talk between Akt and IB kinase activation (
B
and degradation of I
Bs
and ß were not detected in PC12 and SH-SY5Y cells in response to NGF, it is conceivable that activation of I
B kinases via Akt kinase or the Raf/ERK kinase pathway (
B activation in our study. Interestingly, it has recently been demonstrated that the Raf/ERK pathway negatively regulates NF
B-dependent gene expression in cultured fibroblasts (
In summary, our study demonstrates that NGF-induced NFB activity and Bcl-xL expression require tyrosine phosphorylation of I
B
. Activation of NF
B via different ligand-receptor systems and downstream signal transduction pathways may enable the nervous system to maintain constitutive NF
B activity while responding with an increased NF
B activity during injury, inflammation, or repair processes. Moreover, the nonredundancy of NF
B activation pathways emphasizes the importance of this transcription factor for neuronal survival.
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Footnotes |
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1 Abbreviations used in this paper: COX-2, cyclooxygenase-2; EGFP, enhanced green fluorescent protein; NFB, nuclear factor-
B; P-Ser32-I
B
, serine 32-phosphorylated I
B
; RT, reverse transcription; SEAP, secreted form of human placental alkaline phosphatase; TNF-
, tumor necrosis factor-
; TRAF6 dn, dominant negative tumor necrosis factor receptor-associated factor-6.
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Acknowledgements |
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The authors thank Drs. M. Poppe, B. Levkau, and C.M. Luetjens for valuable advice and help with experiments, E. Bauerbach for expert technical assistance, Prof. C.B. Thompson for providing Bcl-x antiserum, Prof. D.W. Ballard for providing plasmid pIB
, and Dr. H. Wajant for plasmid pTRAF6 dn.
This work was supported by the Deutsche Forschungsgemeinschaft (Pr 338/8-1).
Submitted: 8 September 2000
Revised: 4 January 2001
Accepted: 12 January 2001
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References |
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