Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710
Several receptor-mediated signal transduction pathways, including EGF and IgE receptor pathways, have been proposed to be spatially restricted to
plasma membrane microdomains. However, the experimental evidence for signaling events in these microdomains is largely based on biochemical fractionation
and immunocytochemical studies and only little is
known about their spatial dynamics in living cells. Here
we constructed green fluorescent protein-tagged SH2
domains to investigate where and when IgE receptor
(FcRI)-mediated tyrosine phosphorylation occurs in
living tumor mast cells. Strikingly, within minutes after
antigen addition, tandem SH2 domains from Syk or
PLC-
1 translocated from a uniform cytosolic distribution to punctuate plasma membrane microdomains.
Colocalization experiments showed that the microdomains where tyrosine phosphorylation occurred were indistinguishable from those stained by cholera
toxin B, a marker for glycosphingolipids. Competitive
binding studies with coelectroporated unlabeled Syk,
PLC-
1, and other SH2 domains selectively suppressed
the induction of IgE receptor-mediated calcium signals
as well as the binding of the fluorescent SH2 domains.
This supports the hypothesis that PLC-
1 and Syk SH2 domains selectively bind to Syk and IgE receptors, respectively. Unlike the predicted prelocalization of EGF
receptors to caveolae microdomains, fluorescently labeled IgE receptors were found to be uniformly distributed in the plasma membrane of unstimulated cells and
only transiently translocated to glycosphingolipid rich microdomains after antigen addition. Thus, these in
vivo studies support a plasma membrane signaling
mechanism by which IgE receptors transiently associate with microdomains and induce the spatially restricted activation of Syk and PLC-
1.
THE specificity and efficiency of tyrosine kinase-
mediated signal transduction is thought to be controlled by differences in the structure of the catalytic domain that render a kinase selective for particular
substrates and/or by the colocalization of a kinase with its
substrates by direct binding interactions or by cellular
compartmentalization. While enzymatic specificity and direct binding interactions can be studied by various biochemical approaches, much less is known about the dynamic colocalization of different signaling proteins that is
expected to occur in living cells. We reasoned that fluorescently tagged signaling domains could potentially be used
to monitor the spatiotemporal organization of signaling
processes during receptor stimulation of individual cells.
In the following study, we used antigen-stimulated tumor mast cells (RBL cells) as a model system to understand the
local organization of the down stream tyrosine kinase-
mediated signal transduction process.
Antigen-mediated crosslinking of high affinity IgE receptors (Fc Here we used green fluorescent protein (GFP)-tagged
tandem SH2 domains of Syk and phospholipase C- cDNA Constructs and In Vitro RNA Synthesis
The SH2 domains of rat Syk (amino acid Met 1-Glu 265), rat PLC- PLC- Expression and Purification of Recombinant
SH2 Domains
The GST-SH2 domain fusion proteins were expressed in BL21 cells and
purified using a glutathione Sepharose column. The GST tag was removed
by thrombin cleavage, and the SH2 domains were dialyzed in a buffer containing 135 mM NaCl, 5 mM KCl, 20 mM Hepes, pH 7.4, for inhibitory experiments, or in 0.1 M NaHCO3, pH 9.0, for protein labeling.
Fluorescent Labeling of Cholera Toxin B, IgE, and
SH2 Domains
The tandem SH2 domains of PLC- Cell Preparation and Microporation of RNA and
Recombinant Protein
Rat basophilic leukemia cells (2H3 type) were plated on glass coverslips at
least 12 h before experiments and sensitized by incubation with 10 ng/ml
DNP-specific IgE. A small volume electroporation device (a 1-µl microporator [33] was used for loading the proteins and mRNA (~1 µg/µl) into
adherent RBL cells. RBL cells were electroporated at a field strength of
325 V/cm applied for a 40-ms period and repeated three times at 30-s intervals. For the introduction of recombinant SH2 domains, the percent
uptake was determined by coelectroporation of recombinant SH2 domains together with fluo-3, using fluo-3 fluorescence to calibrate the approximate intracellular concentration of SH2 domains (for calibration of
uptake see 23). After loading, cells were washed five times with an extracellular buffer containing 135 mM NaCl, 5 mM KCl, 1.5 mM CaCl2, 1.5 mM MgCl2, 20 mM Hepes, pH 7.4. For experiments with recombinant SH2 domains, cells were left to recover for at least 5 min at 37°C. For RNA transfection, cells were returned to standard medium and placed in the incubator for ~3 h to allow for the expression of GFP-tagged domains. IgE
receptors were activated by addition of variable amounts of DNP-BSA as
indicated in the text.
Fluorescence Imaging and Correlation Analysis
mRNA encoding GFP-tagged SH2 domains and PH domains were electroporated into RBL cells. The expressed proteins were visualized before
and after activation (500 ng/ml DNP-BSA) by confocal laser scanning microscopy (LSM; Zeiss, Inc., Thornwood, NY). Optionally, cells were fixed
after activation and costained with Cy3.5-labeled cholera toxin B subunit.
For costaining experiments of IgE and cholera toxin B, RBL cells were incubated for 1 h at 4°C with 50 ng/ml Cy3.5 or fluorescein-labeled DNP-specific IgE. After a brief incubation at 37°C, the cells were activated at
room temperature (~25°C) with 500 ng/ml DNP-BSA and fixed for 15 min
with paraformaldehyde at different time points after activation. After washing with phosphate-buffered saline, cells were incubated for 10 min with 2 ng/ml fluorescently labeled cholera toxin B and/or 1 µM FM 1-43 (Molecular Probes, Eugene, OR), washed with phosphate-buffered saline, and
the coverslips examined by confocal laser scanning microscopy (LSM;
Zeiss, Inc.).
The overlap in the fluorescence distribution of the two probes was determined by correlation analysis G( Antigen-induced Translocation of GFP-tagged SH2
Domains to Plasma Membrane Microdomains
We studied the spatiotemporal organization of antigen-mediated signal transduction in tumor mast cells (RBL
cells) by using fluorescently tagged SH2 domains as in vivo
signaling probes. GFP-tagged tandem SH2 domains from
Syk and PLC- Addition of antigen triggered the rapid translocation of
the expressed GFP-tagged Syk SH2 domain from a uniform
cytosolic distribution to the plasma membrane (Fig. 1 A).
A similar plasma membrane translocation was observed
for the SH2 domain from PLC-
The time course of the translocation of the Syk SH2-GFP probe to the plasma membrane was monitored in individual cells by series of confocal midsections (Fig. 1 D).
The arrow in Fig. 1 D points to a location where a strong
staining of the plasma membrane developed within less
than 1 min after activation. The time course of the relative
increase in plasma membrane translocated Syk SH2 domains is shown in Fig. 1 E. Within 2 min after antigen addition, 50% of the total number of translocating SH2 domains was associated with the plasma membrane (Fig. 1 E).
We tested whether the plasma membrane translocation
of the GFP-tagged SH2 domains were affected by GFP by
studying the translocation of fluorescently labeled recombinant Syk and PLC-
Selectivity of Syk and PLC- We examined whether the recruitment of Syk and PLC-
We then tested whether the inhibitory effect of the Syk
and PLC- To further evaluate the selectivity of SH2 domains, we
competed the plasma membrane binding of fluorescently
labeled Syk and PLC-
Syk and PLC- The microdomains where tyrosine phosphorylation occurred were further characterized by staining RBL cells
with fluorescently labeled cholera toxin B. Cholera toxin
B is a marker for glycosphingolipids with strong affinity
for GM1 (20) and lower affinity for other gangliosides. A
punctuate plasma membrane staining was observed that
was reminiscent of the one seen for Syk or PLC-
The punctuate plasma membrane staining of cholera
toxin B raises the possibility that these compartments are
identical to the microdomains targeted by the two tandem
SH2 domains. Double staining experiments of GFP Syk
SH2 domains (green) and Cy3.5-labeled cholera toxin B
(red) demonstrated a near complete overlap (yellow), suggesting that GFP-tagged SH2 domains are almost exclusively localized to glycosphingolipid-rich microdomains
(Fig. 5 C). A similar colocalization was observed between
GFP PLC- The colocalization was determined more quantitatively
by a correlation analysis between the plasma membrane
fluorescence intensity line profiles of cholera toxin B and
of GFP-tagged SH2 domains (Fig. 5 E). Cross-correlation
analysis is a convenient method to determine the degree of
overlap between two fluorescent distributions. The higher
the overlap between two distributions, the higher the cross-correlation peak amplitude (at the X-axis value of 0). The autocorrelation analysis of a profile can be used to calculate the maximal peak amplitude that would be observed if
two distributions completely overlapped. The peak amplitudes were 1.26 (Syk SH2 domains; Fig. 5 F) and 1.25 (PLC- Antigen-mediated Cross-linking Leads to the Clustering
of the IgE Receptors
In analogy to the proposed prelocalization of growth factor receptors to caveolae (14, 28, 34), IgE receptors
may be prelocalized to glycosphingolipid-rich regions or,
alternatively, may translocate to these regions only after
receptor activation (1, 6, 7, 24). We measured the plasma
membrane distribution of surface IgE receptors in tumor
mast cells (RBL-2H3) by monitoring fluorescently (Cy3.5)
labeled surface-bound IgE in a confocal microscope. The
high affinity between IgE and the IgE receptor makes fluorescently labeled IgE an ideal receptor marker. Before activation, IgE receptors were uniformly distributed (Fig.
6 A) in the plasma membrane as expected for a freely diffusing surface receptor (27). However, activation with antigen (DNP-BSA) led to the progressive accumulation of
receptors in clusters over a time scale of a few min (Fig. 6 B)
(see reference 30). In the bottom panels, the receptor clustering is shown in one-dimensional plasma membrane fluorescence intensity profiles. Activation leads to a marked
increase in the fluorescence intensity fluctuations along
the plasma membrane, indicating that the receptors become distinctly clustered.
Transient Association of Activated IgE Receptors with
Glycosphingolipid-rich Microdomains
We then tested the possibility that the antigen-induced
clustering of IgE receptors leads to its translocation to glycosphingolipid-rich microdomains. Fig. 7 A shows a coimmunofluoresence staining of RBL cells with IgE-Cy3.5 and
cholera toxin B-FITC before, as well as 3 and 10 min after
crosslinking of the IgE receptor. While the colocalization
between IgE (red) and cholera toxin B (green) was minimal in unstimulated cells (left), antigen addition led to a
significant increase in colocalization within 3 min, as is apparent from the largely yellow membrane staining and loss
of red staining in the overlay image (middle). A few minutes later, the colocalization was progressively lost, even
though the IgE receptors remained clustered (right).
This transient receptor association with glycosphingolipid-rich regions was characterized more quantitatively by a
correlation analysis of the plasma membrane fluorescence
intensity profiles, comparing the cholera toxin B-FITC and
IgE-Cy3.5 distributions. The activation-induced increase
in the correlation peak amplitude between the two distributions supports the hypothesis that IgE receptors translocate to glycosphingolipid-rich microdomains (Fig. 7 B). The
same analysis was then used to measure the time course of
the translocation process. The IgE receptor accumulated
in glycosphingolipid-rich regions for up to 4 min after activation with a t1/2 of <2 min (Fig. 7 C). However, 10 min after activation, the association of IgE receptor with glycosphingolipid-rich regions was markedly decreased. This
suggests that at least two translocation steps are part of the
IgE receptor-mediated signal transduction cascade: a translocation of the IgE receptor from a uniform plasma membrane distribution to glycosphingolipid-rich microdomains, followed by a translocation away from these plasma membrane compartments.
We investigated the spatial and temporal organization of
IgE receptor-mediated signal transduction in living RBL
cells by using fluorescently tagged SH2 domains of Syk
and PLC- Our studies showed that the subcompartments stained
with cholera toxin subunit B have a marked overlap with
the compartments stained with the fluorescently tagged
SH2 domains. This suggests that the IgE receptor as well
as Syk are specifically tyrosine phosphorylated in microdomains that contain glycosphingolipids. The existence of
separate signaling compartments in the plasma membrane
of RBL cells is supported by the earlier finding that the
crosslinked IgE receptor associates with a detergent-insoluble cell fractions in RBL cells (7, 26). The latter study of Field et al. (7) showed that the cross-linked receptor appears in a detergent insoluble cell fraction within 2 to 3 min after antigen addition. Furthermore, this translocation
to the detergent-insoluble fraction occurred in parallel
with the tyrosine phosphorylation of the receptor, a process that was maximal after 3 min and subsequently decreased to <50% between 5 and 30 min. While this time
course is similar to the one observed in our study, the data
in our Fig. 1 D suggest a longer presence of SH2 domains
at the plasma membrane. A possible explanation for the
prolonged plasma membrane association of the Syk SH2
domain is a protective role of the SH2 domain in the dephosphorylation of the IgE receptor. In addition, a similar protective mechanism may also be responsible for an observed reduced receptor internalization in the presence of
Syk SH2 domains within the first 15 min after antigen addition (i.e., Fig. 1 D).
While our in vivo studies with SH2 domains do not directly show a translocation of Syk and PLC- Previous studies have shown that IgE receptor crosslinking leads to the Lyn-mediated phosphorylation of
ITAM motifs on its While the localized signaling through EGF, PDGF, and
NGF receptors has been proposed to be mediated by their
prelocalization to caveolae-type membrane compartments
(8, 18, 22, 34), we here show that IgE receptors are uniformly localized in the plasma membrane and only transiently align with compartments stained with cholera toxin
B during the activation process. This two step process for
IgE receptor activation, a translocation of the IgE receptor from a uniform plasma membrane distribution to glycosphingolipid-rich microdomains, followed by a translocation away from these plasma membrane compartments
a few minutes later, is likely to represent an important signaling principle by which receptors can activate signaling
processes in a spatially restricted manner.
Overall, these studies show that IgE receptor-mediated
signal transduction is a compartmentalized process that
can be monitored in living cells. Furthermore, our studies
suggest that the transient association of IgE receptors with
glycosphingolipid-rich microdomains, and the sequential
activation of IgE receptor, Syk, and PLC-RI) is a critical step for triggering the release
of inflammatory agents from mast cells (12). Previous studies have shown that initial steps for mast cell activation include the Lyn-mediated tyrosine phosphorylation of IgE
receptors on immunoreceptor tyrosine-based activation
motifs (ITAM's; 5, 19, 25, 29, 35) and the binding of the
tandem SH2 domains of tyrosine kinase Syk to these motifs (2, 13, 31). Furthermore, activation of Syk can trigger calcium signals by activation of phospholipase C (PLC)1-
1
and production of inositol 1,4,5-trisphosphate (InsP3; 3). Calcium signals, together with the diacylglycerol-mediated
activation of protein kinase C, have been shown to be important regulators for the secretion of histamine, the synthesis of prostaglandins, leukotrienes, and the expression
of cytokines (12).
1 as in
vivo signaling probes to monitor Fc
RI phosphorylation
and Syk phosphorylation, respectively. We show that both
tandem SH2 domains translocate from a uniform cytosolic
distribution to punctuate plasma membrane microdomains
after antigen stimulation. These membrane subcompartments were identified as glycosphingolipid-rich microdomains by the colocalization of GFP-tagged SH2 domains
with fluorescent cholera toxin B. A surprising finding was
also that fluorescently labeled IgE receptors were uniformly distributed in unstimulated cells, and only antigen
stimulation induced a transient association of IgE receptor
with plasma membrane microdomains. Overall, our studies show that GFP-tagged SH2 domains are powerful in
vivo probes to monitor the localized phosphorylation of
selective tyrosine residues in individual cells and that the
transient association of receptors with microdomains is a
possible mechanism by which receptors can selectively activate downstream targets.
Materials and Methods
1 (Ser
539-Gly 777), and Pleckstrin PH domain (Met 1-Gly 105) were subcloned
3
to cycle3 GFP (4) into the eukaryotic RNA expression vector Hiro3
(36). An additional S65T mutation was added to cycle3 GFP to increase
its brightness (10). The RNA was transcribed and polyadenylated by sequential in vitro steps as described in 36.
1 and Syk tandem SH2 domains were subcloned into the expression vector pGex2T (Pharmacia Biotech, Piscataway, NJ) by PCR-mediated mutagenesis using primers containing additional restriction sites.
Tandem SH2 domains from Syk were cloned between codons 1 (Met) and
256 (Glu) and tandem SH2 domains from PLC-
1 between codons 546 (Ser) and 791 (Thr). The glutahione-S-transferase (GST) fusion constructs
of SH2 domain from Abl and phosphatydilinositol (PI)3-kinase were
kindly provided by Dr. A.-M. Pendergast (Duke University).
1 and Syk were labeled with Cy2
(Amersham Intl., Arlington Heights, IL). Anti-dinitrophenyl (DNP)-IgE
(Sigma Chemical Co., St Louis, MO) and -cholera toxin B subunit were labeled with Cy3.5. The cholera toxin B subunit labeled with FITC was purchased from Sigma Chemical Co. The labeling reactions were performed
for 1 h at room temperature in 0.1 M NaHCO3, pH 9.0, with a twofold molar ratio of fluorescent dye to protein. The labeled proteins were separated from the unbound dye by gel filtration chromatography. The SH2
domains and the cholera toxin B subunit were labeled in a ratio of 1:2 fluorescent labels per molecules, whereas four labels were conjugated per IgE.
x) = l n
i = lf(i) × g(i +
x) with f(i) as
the fluorescence intensity in the green and g(i) as the fluorescence intensity in the red channel. For this analysis, NIH Image 1.6 software was used
to obtain the two line profiles of the plasma membrane fluorescence intensity in the red and green channels. Confocal midsections of cells were
used, and the entire cell boundary around each cell was tracked in parallel
for both channels.
Results
1 were expressed by microporation their in
vitro transcribed and poly adenylated RNA in RBL cells,
which allows for the rapid and efficient expression of fusion proteins in adherent cells (33, 36).
1, while SH2 domains of signaling molecules not involved in this pathway (i.e., PI3
Kinase, Abl, or Grb2) failed to translocate after antigen stimulation (data not shown). The Syk and PLC-
1 SH2 domains
showed a marked nonuniformity in plasma membrane localization. This clustering of SH2 domains in plasma membrane microdomains is shown more clearly in a plasma
membrane surface image in Fig. 1 B and in a line intensity
profile in Fig. 1 C.
Fig. 1.
Cross-linking of IgE receptor leads to the translocation
of GFP-tagged Syk tandem SH2 domains from the cytosol to distinct plasma membrane microdomains. (A) GFP-Syk SH2 domains
were expressed in RBL cells by RNA transfection to monitor the
translocation of Syk SH2 domains in vivo. A midsection of the
same cell is shown before and 3 min after the addition of antigen
(500 ng/ml DNP-BSA). (B) Confocal section of the cell surface 3 min after antigen addition. The same experimental conditions as
in A were used. (C) One-dimensional plasma membrane fluorescence intensity profiles for a midsection segment before and after
activation with antigen. (D) The time course of the plasma membrane translocation of the Syk SH2 domains GFP probe is shown
in a confocal midsection of the same cell. The arrow points to one
of the sites where a strong punctate membrane staining appears.
(E) Plot of the increase of the total plasma membrane fluorescence intensity at different time points after activation of the cells
with antigen (average of four cells). Bar, 10 µm.
[View Larger Version of this Image (55K GIF file)]
1 tandem SH2 domains. Recombinant SH2 domains were labeled with Cy2 and loaded into
RBL cells by electroporation. Fig. 2 A shows that also Cy2-labeled SH2 domains translocated to the plasma membrane
after antigen addition. We also determined whether the
punctate distribution of translocated GFP-tagged SH2
domains is a result of membrane inhomogeneities by
monitoring the distribution of plasma membrane-localized GFP-tagged PH domain from pleckstrin during antigen
stimulation. This PH domain binds to phosphatidylinositol
4,5-bisphosphate (PIP2), which is predominantly present
in the plasma membrane (9). The expressed PH domains
were uniformly associated along the plasma membrane
and did not significantly redistribute in activated cells (Fig.
2 B). A comparison of line intensity profiles of Syk SH2
and PH domains along the plasma membrane (Figs. 1 C and 2 C) suggests that the punctuate staining observed for
Syk and PLC-
1 SH2 domains is indeed the result of a
translocation to specific plasma membrane microdomains.
Fig. 2.
Control measurements, showing that punctate plasma
membrane staining can also be seen after antigen addition with
fluorescently labeled recombinant Syk SH2 domains but not with
the GFP-tagged phospholipid binding pleckstrin-PH domain. (A)
The translocation of Syk SH2 domains was also observed when
recombinant Syk SH2 domains were labeled with Cy2 and introduced into RBL cells by microporation. The images show cells
that were fixed 3 min after antigen addition (to minimize the cytosolic background staining). The stimulation conditions were the
same as in Fig. 1 A. (B) Plasma membrane localization of GFP-pleckstrin PH domain in living cells 3 min after stimulation. Antigen activation had no significant effect on the distribution of PH
domains. (C) One-dimensional plasma membrane fluorescence
intensity profiles for a membrane segment of cells that expressed
GFP-tagged pleckstrin PH domain. Bar, 10 µm.
[View Larger Versions of these Images (80 + 9K GIF file)]
SH2 Domains
1
SH2 domains is required for IgE receptor-mediated calcium signaling since earlier studies have suggested that
calcium signaling is mediated by Syk and PLC-
1 activation (3). The effect of the SH2 domains on IgE receptor-
mediated calcium signaling was tested by microporation of
recombinant tandem SH2 domains from Syk and PLC-
1
and single SH2 domains from Abl and PI3-kinase into RBL cells (Fig. 3 A). Syk and PLC-
1 SH2 domains, but not the
SH2 domains from Abl and PI3-kinase, inhibited calcium
signaling in a concentration-dependent manner (Fig. 3, A
and B). Half-maximal inhibition was observed at ~200 nM
of intracellular PLC-
1 or Syk SH2 domains. This result is
consistent with the hypothesis that SH2 domain interactions from Syk and PLC-
1, but not those of Abl and PI3-kinase, are critical signaling steps for the induction of IgE
receptor-mediated calcium spikes.
Fig. 3.
Importance of Syk
and PLC-1 SH2 domains for
antigen-mediated calcium
signaling. (A) IgE receptor-
mediated calcium spiking is
progressively inhibited at increasing concentrations of
recombinant Syk SH2 domains. RBL cells were coelectroporated with fluo-3
and SH2 domains, and intracellular concentrations of
SH2 domains were estimated
by the relative fluorescence
intensity of coelectroporated fluo3 fluorescence (see reference 32 for calibration). Calcium spikes were recorded as a
function of time after crosslinking of IgE receptor with
DNP-BSA (50 ng/ml). The
shown fluorescence intensity
traces reflect calcium responses after IgE receptor activation in the presence of
different concentrations of
Syk SH2 domains. (B) Concentration-dependent inhibition of calcium spiking by
Syk (squares) and PLC-
1
SH2 domains (circles). SH2 domains from Abl (triangle)
and PI3 kinase (cross) did not affect IgE receptor-mediated calcium signaling even at maximal concentrations. (C) The triggering of G protein-coupled calcium signals is not affected by recombinant Syk or PLC-
1 SH2 domains. RBL cells with stably
transfected FMLP receptors were activated with FMLP (10 µM)
or with antigen (50 ng/ml) at different time points. Different protocols are shown with either antigen added alone, FMLP added alone, or both added sequentially (with the FMLP addition 4 min after the antigen addition).
[View Larger Versions of these Images (28 + 26K GIF file)]
SH2 domains on calcium signaling is specific
for the IgE receptor pathway by electroporating recombinant SH2 domains into RBL cells with stably transfected
FMLP receptor. Calcium signaling triggered by this G protein-coupled receptor remained unaffected in the presence of the SH2 domains, while the same cells failed to respond to antigen stimulation (Fig. 3 C).
1 SH2 domains in the presence of
either unlabeled SH2 domains of Syk or PLC-
1. While
Syk SH2 domains prevented almost completely the translocation of the fluorescent Syk SH2 domain, the SH2 domains of PLC-
1 failed to do so even at maximal concentrations (Fig. 4). In contrast, SH2 domains from Syk and
PLC-
1 both inhibited the translocation of fluorescently
labeled PLC-
1 SH2 domains to the plasma membrane.
These measurements are consistent with the hypothesis
that SH2 domains from Syk and PLC-
1 are specific in
vivo probes for phosphorylated ITAM motifs of IgE receptors and for phosphorylated Syk, respectively.
Fig. 4.
Syk and PLC-1 SH2 domains bind to different binding
partners. (A) The translocation of fluorescently labeled Syk SH2
domain to the plasma membrane (left) is suppressed in the presence of high concentrations of unlabeled Syk SH2 domains (middle) but not suppressed in the presence of high concentrations of
unlabeled PLC-
1 SH2 domains (right). (B) Fluorescently labeled PLC-
1 SH2 domains translocate to the plasma membrane
(left) upon stimulation with antigen. The translocation is suppressed in the presence of high concentrations of unlabeled Syk
(middle) or PLC-
1 SH2 domains (right). Bar, 10 µm.
[View Larger Version of this Image (80K GIF file)]
1 SH2 Domains Bind to
Glycosphingolipid-rich Microdomains
1 SH2
domains (Fig. 5 A). Again, the punctuate staining is most
clearly apparent in a surface section (Fig. 5 A, right). As an
additional control experiment, we found that the plasma
membrane marker FM 1-43 stained the cell surface uniformly in live and fixed cells (Fig. 5 B). This suggests that
the punctuate staining observed with cholera toxin is not
due to the fixation of the cells.
Fig. 5.
Colocalization of
GFP-tagged Syk and PLC-1
SH2 domains with the glycosphingolipid marker cholera
toxin B in antigen stimulated RBL cells. (A) Cholera toxin
B staining in a midsection (left) and surface section (right) of
unstimulated RBL cells. (B)
Plasma membrane staining of
unstimulated RBL cells with
FM 1-43. (C) Colocalization
of GFP-Syk SH2 domain
(green) and cholera toxin B
Cy3.5 (red) in RBL cells 3 min
after cross-linking of IgE receptors with 500 ng/ml DNP-BSA. The color yellow in the
overlay image on the right indicates a colocalization of SH2
domains and cholera toxin B. (D) Same as in C but with
GFP-PLC-
1 SH2 domain instead of Syk SH2 domains. (E) One-dimensional plasma membrane fluorescence intensity profiles of the GFP-Syk SH2 domains and
cholera toxin B-Cy3.5 of the cell shown in C. (F) Correlation analysis of cells costained with the Syk-SH2 and cholera toxin B probes 3 min after antigen activation (average of five cells). (G) Correlation analysis of cells costained with the PLC-
1 SH2 domain and cholera
toxin B probes 3 min after antigen activation (average of six cells). The relatively high correlation peak in the traces in E and F indicates
a high degree of colocalization in the two images. (F and G) As a control, a comparison of the colocalization of cholera toxin B with
GFP-tagged PH domains and FM 1-43 was included in F and G, respectively. Bars, 10 µm.
[View Larger Versions of these Images (40 + 29K GIF file)]
1 SH2 domains and cholera toxin B (Fig. 5 D).
1 SH2 domains; Fig. 5 G) for the cross-correlation
between the two profiles, compared to 1.28 for their autocorrelation. This is consistent with a near complete colocalization of cholera toxin B and GFP-SH2 domains. In
contrast, the correlation between the distributions of cholera toxin B and GFP-tagged PH domains or FM 1-43 was
much smaller (Fig. 5, F and G). These results strongly suggest that the recruitment of Syk and PLC-
1 SH2 domains
is confined to glycosphingolipid-rich microdomains.
Fig. 6.
IgE receptors (FcRI) redistribute from a uniform to a
punctuate plasma membrane distribution after antigen activation. (A) Distribution of fluorescently labeled IgE before and after antigen activation. Confocal image of a midsection through an
RBL-cell before (left) and 3 min after (right) stimulation with the
500 ng/ml of antigen (DNP-BSA). (B) Line intensity profiles of
the plasma membrane fluorescence intensity are shown for the
two images. Bar, 10 µm.
[View Larger Version of this Image (62K GIF file)]
Fig. 7.
Time course of association of fluorescently marked IgE
receptor with glycosphingolipid-rich microdomains after antigen
addition. (A) Comparison of the distributions of IgE receptors
and cholera toxin B. Overlay images of IgE receptor (red) and
cholera toxin B (green) are shown as a function of time after stimulation with 500 ng/ml antigen. (Left) Minimal colocalization is
observed before antigen activation. (Middle) A significant overlap
of IgE receptor and cholera toxin B is observed after 3 min. (Right)
The colocalization is reduced 10 min after activation. Yellow shows
increased overlap in cholera toxin B and IgE distribution while
the red regions show the IgE receptor distributed elsewhere. (B)
Correlation analysis between cholera toxin B distribution before
and 3 min after activation (average of four cells). (C) Time course
of the increase and subsequent decrease of the correlation peak
amplitudes. Data are expressed as the mean of replicate samples
from at least six cells ± SD. The dotted line corresponds to the
cross correlation peak value of the PH domain with cholera toxin
B. Maximal colocalization of IgE receptors with glycosphingolipid-rich membrane compartments is observed 2-4 min after
activation.
[View Larger Versions of these Images (17 + 21K GIF file)]
Discussion
1. The recruitment of Syk and PLC-
1 SH2 domains to distinct plasma membrane microdomains strongly
supports the hypothesis that IgE receptor-mediated signal
transduction is spatially restricted. Src family kinases such
as Lyn, tyrosine kinase receptors, and other signaling proteins have been shown by biochemical studies to be localized to the detergent-insoluble plasma membrane fractions
that have been termed caveolae or detergent-insoluble
glycosphingolipid-enriched complexes (DIGs) (14, 28,
34). Could these microdomains stained by the SH2 domains be related to caveolae or DIGs? While the microdomains show variations in morphology and detergent solubility, they all contain a particular lipid composition (e.g.,
glycosphingolipids, sphingomyelins, and cholesterol [21]).
Due to the lack of caveolae, and the caveolae marker caveolin in RBL cells (6, 7), we used cholera toxin subunit B
to visualize the localization of potential lipid microdomains. Cholera toxin B binds to ganglioside GM1 and
with lower affinity to other gangliosides. Earlier studies in
RBL cells have shown a more homogenous distribution of
a GD1b-specific antibody AA4 (17, 23), suggesting that
cholera toxin B-labeled gangliosides and GD1b gangliosides are not equally distributed.
1 holoenzyme, our studies suggest that a tyrosine phosphorylation-
mediated activation of Syk and PLC-
1 is essential for IgE
receptor-mediated calcium signaling. Nevertheless, it is important to consider possible differences in the localization
between the full length protein and its SH2 domain, since
full length Zap-70, a tyrosine kinase related to Syk (11),
was already prelocalized to a near plasma membrane structure before activation.
and
subunits (5, 19, 25, 29, 35). The
selective and quantitative inhibition of IgE receptor-
mediated calcium signaling by Syk-SH2 domains (Fig. 3),
together with the earlier finding that the SH2 domain of
Syk selectively binds to ITAM motifs (2, 13, 31), supports
the hypothesis that Syk has an essential function in IgE
receptor-mediated calcium signaling. Similarly, the quantitative inhibition of calcium signaling by PLC-
1 SH2 domains suggests that the Syk-mediated activation of PLC-
1 is the main pathway in RBL cells to generate calcium
signals (3). We also evaluated the specificity of SH2 domains targets by showing that unlabeled PLC-
1 prevents
the binding of fluorescent PLC-
1 but not that of fluorescent Syk SH2 domains. In a different set of experiments,
we showed that fluorescently labeled Syk SH2 domains do
not bind to the tyrosine-phosphorylated PDGF or EGF
receptors in NIH-3T3 cells or A431-epithelial cells, whereas
activation of both receptors induced a plasma membrane
translocation of fluorescently tagged PLC-
1 SH2 domains
(data not shown). Thus, our studies strongly support the
hypothesis that the phosphorylation of IgE receptor and
Syk are functionally important for the recruitment of Syk
and PLC-
1, respectively. Our studies also suggest that different fluorescent SH2 domains can be used as tools to
selectively monitor the tyrosine phosphorylation of distinct signaling proteins in different cell types.
1 within these
microdomains, defines the specificity and efficiency of antigen-mediated mast cell activation.
Received for publication 16 July 1997 and in revised form 24 August 1997.
T.P. Stauffer is a recipient of a fellowship from the Swiss National Science Foundation (Grant 823A-050457). T. Meyer was supported by a fellowship from the David and Lucile Packard Foundation. This work was supported by Proctor & Gamble grant SRA 1617 and the National Institutes of Health grants GM-48113 and GM-51457.We thank Drs. A.-M. Pendergast and C. Nicchitta for critical reading of
the manuscript and Hiroko Yokoe, Kang Shen, and Drs. J. Horne, M. Teruel, and K. Subramanian for stimulating discussions. We thank Drs. J. Brugge (Syk), S.G. Rhee (PLC-), and S. Feisik (pleckstrin) for providing
the corresponding cDNA constructs.
GFP, green fluorescent protein; GST, glutathione-S-transferase; ITAM, immunoreceptor tyrosine-based activation motifs; PI, phosphatydilinositol; PLC, phospholipase C.
1. |
Apgar, J.R..
1990.
Antigen-induced cross-linking of the IgE receptor leads
to an association with the detergent-insoluble membrane skeleton of rat
basophilic leukemia (RBL-2H3) cells.
J. Immunol.
145:
3814-3822
|
2. |
Benhamou, M.,
N.J. Ryba,
H. Kihara,
H. Nishikata, and
R.P. Siraganian.
1993.
Protein-tyrosine kinase p72syk in high affinity IgE receptor signaling. Identification as a component of pp72 and association with the receptor ![]() |
3. |
Choi, O.H.,
J.H. Lee,
T. Kassessinoff,
J.R. Cunha-Melo,
S.V. Jones, and
M.A. Beaven.
1993.
Antigen and carbachol mobilize calcium by similar
mechanisms in a transfected mast cell line (RBL-2H3 cells) that expresses ml muscarinic receptors.
J. Immunol.
151:
5586-5595
|
4. | Crameri, A., E.A. Whitehorn, E. Tate, and P.C. Stemmer. 1996. Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat. Biotechnol. 14: 315-319 . |
5. | Eiseman, E., and J.B. Bolen. 1992. Engagement of the high-affinity IgE receptor activates src protein-related tyrosine kinases. Nature. 355: 78-80 |
6. |
Field, K.A.,
D. Holowka, and
B. Baird.
1995.
Fc![]() |
7. |
Field, K.A.,
D. Holowka, and
B. Baird.
1997.
Compartmentalized activation of the high affinity immunoglobulin E receptor within membrane
domains.
J. Biol. Chem.
272:
4276-4280
|
8. |
Fra, A.M.,
E. Williamson,
K. Simons, and
R.G. Parton.
1994.
Detergent-
insoluble glycolipid microdomains in lymphocytes in the absence of caveolae.
J. Biol. Chem.
269:
30745-30748
|
9. | Harlan, J.E., H.S. Yoon, P.J. Hajduk, and S.W. Fesik. 1995. Structural characterization of the interaction between a pleckstrin homology domain and phosphatidylinositol 4,5-bisphosphate. Biochemistry. 34: 9859-9864 |
10. | Heim, R., and R. Tsien. 1995. Improved green fluorescence. Nature. 373: 663-664 |
11. |
Huby, R.D.J.,
M. Iwashima,
A. Weiss, and
S.C. Ley.
1997.
ZAP-70 protein
tyrosine kinase is constitutively targeted to the T cell cortex independently of its SH2 domains.
J. Cell Biol.
137:
1639-1649
|
12. |
Jouvin, M.H.,
R.P. Numerof, and
J.P. Kinet.
1995.
Signal transduction
through the conserved motifs of the high affinity IgE receptor Fc![]() |
13. |
Kihara, H., and
R.P. Siraganian.
1994.
Src homology 2 domains of Syk and
Lyn bind to tyrosine-phosphorylated subunits of the high affinity IgE receptor.
J. Biol. Chem.
269:
22427-22432
|
14. |
Liu, P.,
Y. Ying,
Y.G. Ko, and
R.G. Anderson.
1996.
Localization of platelet-derived growth factor-stimulated phosphorylation cascade to caveolae.
J. Biol. Chem.
271:
10299-10303
|
15. |
Liu, J.L.,
P. Oh,
T. Horner,
R.A. Rogers, and
J.E. Schnitzer.
1997.
Organized endothelial cell surface signal transduction in caveolae distinct
from glycophosphatitylinositol-anchored protein microdomains.
J. Biol.
Chem.
272:
7211-7222
|
16. |
Mineo, C.,
G.L. James,
E.J. Smart, and
R.G. Anderson.
1996.
Localization
of epidermal growth factor-stimulated Ras/Raf-1 interaction to caveolae
membrane.
J. Biol. Chem.
271:
11930-11935
|
17. |
Oliver, C.,
N. Sahara,
S. Kitani,
A.R. Robbins,
L.M. Mertz, and
R.P. Siraganian.
1992.
Binding of monoclonal antibody AA4 to gangliosides on
rat basophilic leukemia cells produces changes similar to those seen with
Fc![]() |
18. | Palade, G.E.. 1953. Fine structure of blood capillaries. J. Appl. Physics. 24: 1424 . |
19. | Paolini, R., R. Numerof, and J.P. Kinet. 1994. Kinase activation through the high-affinity receptor for immunoglobulin E. Immunomethods. 4: 35-40 |
20. |
Parton, R.G..
1994.
Ultrastructural localization of gangliosides; GM1 is concentrated in caveolae.
J. Histochem. Cytochem.
42:
155-166
|
21. | Parton, R.G.. 1996. Caveolae and caveolins. Curr. Opin. Cell Biol. 8: 542-548 |
22. | Parton, R.G., and K. Simons. 1995. Digging into caveolae. Science. 269: 1398-1399 |
23. |
Pierini, L.,
D. Holowka, and
B. Baird.
1996.
Fc![]() |
24. |
Pribluda, V.S.,
C. Pribluda, and
H. Metzger.
1994.
Transphosphorylation as
the mechanism by which the high-affinity receptor for IgE is phosphorylated upon aggregation.
Proc. Natl. Acad. Sci. USA.
91:
11246-11250
|
25. | Reth, M.. 1989. Antigen receptor tail clue. Nature. 338: 383-384 |
26. |
Robertson, D.,
D. Holowka, and
B. Baird.
1986.
Cross-linking of immunoglobulin E-receptor complexes induces their interaction with the cytoskeleton of rat basophilic leukemia cells.
J. Immunol.
136:
4565-4572
|
27. | Ryan, T.A., J. Myers, D. Holowka, B. Baird, and W.W. Webb. 1988. Molecular crowding on the cell surface. Science. 239: 61-64 |
28. | Sargiacomo, M., M. Sudol, Z. Tang, and M.P. Lisanti. 1993. Signal transducing molecules and glycosyl-phosphatidylinositol-linked proteins form a caveolin-rich insoluble complex in MDCK cells. J. Cell Biol. 122: 789-807 [Abstract]. |
29. | Scharenberg, A.M., S. Lin, B. Cuenod, H. Yamamura, and J.P. Kinet. 1995. Reconstitution of interactions between tyrosine kinases and the high affinity IgE receptor which are controlled by receptor clustering. EMBO (Eur. Mol. Biol. Organ.) J. 14: 3385-3394 [Abstract]. |
30. | Seagrave, J., J.R. Pfeiffer, C. Wofsy, and J.M. Oliver. 1991. Relationship of IgE receptor topography to secretion in RBL-2H3 mast cells. J. Cell. Physiol. 148: 139-151 |
31. |
Shiue, L.,
J. Green,
O.M. Green,
J.L. Karas,
J.P. Morgenstern,
M.K. Ram,
M.K. Taylor,
M.J. Zoller,
L.D. Zydowsky,
J.B. Bolen, et al
.
1995.
Interaction of p72syk with the ![]() ![]() ![]() |
32. | Stauffer, T.P., C.H. Martenson, J.E. Rider, B.K. Kay, and T. Meyer. 1997. Inhibition of Lyn function in mast cell activation by SH3 domain binding peptides. Biochemistry. 36: 9388-9394 |
33. | Teruel, M.N., and T. Meyer. 1997. Electropopration induced formation of individual calcium entry sites in cell body and processes of adherent cells. Biophys. J. 73: 1785-1796 [Abstract]. |
34. |
Wu, C.,
S. Butz,
Y.-s. Ying, and
R.G.W. Anderson.
1997.
Tyrosine kinase
receptor concentrated in caveolae like domains from neuronal plasma
membrane.
J. Biol. Chem.
272:
3554-3559
|
35. |
Yamashita, T.,
S.Y. Mao, and
H. Metzger.
1994.
Aggregation of the high-affinity IgE receptor and enhanced activity of p53/56lyn protein-tyrosine
kinase.
Proc. Natl. Acad. Sci. USA.
91:
11251-11255
|
36. | Yokoe, H., and T. Meyer. 1996. Spatial Dynamics of GFP-tagged proteins investigated by local fluorescence enhancement. Nat. Biotechnol. 14: 1252-1256 . |