Correspondence to Peter J. Lockyer: peter.lockyer{at}bbsrc.ac.uk
Abstract
The versatility of Ca2+ as a second messenger lies in the complex manner in which Ca2+ signals are generated. How information contained within the Ca2+ code is interpreted underlies cell function. Recently, we identified CAPRI and RASAL as related Ca2+-triggered Ras GTPase-activating proteins. RASAL tracks agonist-stimulated Ca2+ oscillations by repetitively associating with the plasma membrane, yet CAPRI displays a long-lasting Ca2+-triggered translocation that is refractory to cytosolic Ca2+ oscillations. CAPRI behavior is Ca2+- and C2 domaindependent but sustained recruitment is predominantly Ca2+ independent, necessitating integration of Ca2+ by the C2 domains with agonist-evoked plasma membrane interaction sites for the pleckstrin homology domain. Using an assay to monitor Ras activity in real time, we correlate the spatial and temporal translocation of CAPRI with the deactivation of H-Ras. CAPRI seems to low-pass filter the Ca2+ signal, converting different intensities of stimulation into different durations of Ras activity in contrast to the preservation of Ca2+ frequency information by RASAL, suggesting sophisticated modes of Ca2+-regulated Ras deactivation.
Introduction
Ras is a key player in cell signaling with a central role in cancer. Oncogenic mutants are locked in the GTP-bound conformation resistant to GTPase-activating proteins (GAPs). We recently discovered closely related Ca2+-triggered GAPs; RASAL (Allen et al., 1998; Walker et al., 2004) and CAPRI (Lockyer et al., 2001). Each contains tandem C2 domains (C2A and C2B) and a GAP-related domain (GRD) flanked by a pleckstrin homology (PH) domain and Bruton's tyrosine kinase (Btk) motif. RASAL is a Ca2+ sensor responding in-phase to repetitive Ca2+ signals by associating with the plasma membrane and deactivating Ras (Walker et al., 2004). Here, we compare the behavior of CAPRI with that of RASAL and PKC after G proteincoupled receptor stimulation. Studies of conventional PKC isoforms have resolved a frequency-dependent activation mechanism reliant on oscillatory associations with the plasma membrane in concert with fluctuations in intracellular Ca2+ (via the C2 domain) and DAG (via the C1 domain) (Oancea and Meyer, 1998; Violin et al., 2003). RASAL also tracks Ca2+ oscillations (Walker et al., 2004). However, CAPRI is a novel low-pass filter for Ca2+, dependent on predominantly Ca2+-independent interactions between the plasma membrane and the PH domain after receptor activation.
Results and discussion
Spatial and temporal regulation of CAPRI by agonist-evoked Ca2+ signals
RASAL and PKC are sensors of repetitive Ca2+ signals (Oancea and Meyer, 1998; Violin et al., 2003; Walker et al., 2004), and we wondered if CAPRI would behave similarly. We tested responses of GFP-CAPRI, GFP-RASAL, and GFP-PKC
to histamine stimulation of HeLa cells. This demonstrated long-term association of GFP-CAPRI with the plasma membrane at supra-maximal doses of agonist (Fig. 1, A and B). Half-maximal dissociation back to the cytosol was >280 s, contrasting with half-maximal dissociation of 17 s for GFP-RASAL and 13 s for GFP-PKC
(Fig. 1 C). A 10-µM dose evoked similar CAPRI translocation to the membrane as 100 µM histamine, but with a faster rate of dissociation (Fig. 1 B; half-maximal dissociation 88 s).
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CAPRI does not suppress Ca2+ oscillations, but is refractory to them
Dawson first demonstrated that Ca2+ release could be stimulated by GTP and blocked by nonhydrolyzable GTP analogues (Dawson, 1985). Evidence has since accumulated that small GTPases are involved in the regulation of Ca2+ entry (Bird and Putney, 1993). A direct role for oncogenic Ras in regulating Ca2+ spike frequency has been shown in NIH3T3 cells (Lang et al., 1991). We therefore wondered whether CAPRI expression altered Ca2+ responses. In an earlier study, we generated a series of CHO cell lines stably expressing CAPRI or a CAPRI GRD mutant (R473S; GAP dead) (Lockyer et al., 2001). To determine if CAPRI expression had any impact on agonist-stimulated Ca2+ signals, we monitored cytosolic free Ca2+ in single cells. Supra-maximal ATP stimulation produced similar averaged Ca2+ peakplateau transients between wild-type or mutant cells showing that Ras GAP function was not interfering with maximal Ca2+ responses with this agonist and cell type (Fig. 2 A). It did not exclude the possibility that CAPRI inhibits Ca2+ oscillations at lower agonist doses. To determine if CAPRI expression blocked Ca2+ oscillations in HeLa cells, we performed sequential imaging of GFP-CAPRI and Fura-2 (Fig. 2, B and C). As shown in Fig. 2 B, during sinusoidal Ca2+ signals there was a prolonged recruitment of CAPRI over a period of 500 s. This contrasted with the in-phase translocations of GFP-RASAL recently measured (Walker et al., 2004) and studies reported for other C2 domaincontaining Ca2+ sensors such as conventional PKCs (Oancea and Meyer, 1998; Violin et al., 2003). Ca2+ spikes induced by a low dose of histamine generated weak, or typically more transient, CAPRI translocation (Fig. 2 C). At a submaximal histamine dose (1 µM) this was difficult to detect; by conventional confocal imaging only 5% of GFP-CAPRIexpressing cells displayed clear translocations (n = 60 cells), compared with 88% at 50 µM histamine (n = 75). During sequential GFP-CAPRI and Fura-2 imaging we detected no significant differences in spike periodicity and peak dye emission between transfected or nontransfected cells in the same field (unpublished data). Sequential confocal imaging of Fluo-4 and RFP-CAPRI also demonstrated that CAPRI does not faithfully track histamine-evoked changes in cytosolic Ca2+ (Fig. 2 D). As shown in Fig. 2 E, the translocation of GFP-CAPRI to the plasma membrane was dependent on agonist, was not cumulative, and displayed reversibility. A repetitive application of 50 µM histamine for 60 s followed by washing and then reapplication after 180 s generated transient Ca2+ mobilization and transient CAPRI membrane association. We cotransfected HeLa cells with CAPRI-YFP and CFP-RASAL and stimulated them with ATP to prove that CAPRI and RASAL show different responses to the same stimulus, in the same cell (Fig. 2 F). Where RASAL oscillated, CAPRI translocated in a sustained manner.
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Role of the tandem C2 domains and PH domain for membrane translocation
We showed previously that the C2A and C2B domains of CAPRI in tandem (C2AB) are necessary and sufficient for sensing an increase in cytosolic Ca2+ (Lockyer et al., 2001). GFP-C2AB has a tendency to concentrate in the nucleoplasm. As shown in Fig. 3 A, ionomycin caused the rapid translocation of GFP-C2AB to the nuclear membrane in a Ca2+-dependent manner. Translocation to the plasma membrane was also detected in cells where cytosolic GFP-C2AB was sufficiently high. It seemed likely that electrostatic and lipid headgroup interactions were necessary for C2 domaindriven translocation, as is the case for other C2 domaincontaining Ca2+ sensors (Rizo and Sudhof, 1998). In support of a role for electrostatic interactions in dictating membrane specificity, we noted that histamine failed to induce the nuclear translocation of GFP-C2AB but did cause translocation of cytosolic protein to the plasma membrane (Fig. 3 A). Ionomycin generates a sustained, high amplitude Ca2+ elevation, so it seems likely that the GFP-C2AB domain loses membrane specificity under chronically high Ca2+ conditions.
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Total internal reflection fluorescence microscopy detects CAPRI oscillations
Total internal reflection fluorescence microscopy (TIRFM) offers superior observation of fluorescent molecules at the cell surface. Imaging GFP-CAPRI responses to histamine by TIRFM showed CAPRI translocation to be peakplateau (Fig. 4 A; Video 1, available at http://www.jcb.org/cgi/content/full/jcb.200504167/DC1). The initial spike of translocation was more apparent than by Nipkow confocal imaging (Fig. 1). GFP-RASAL exhibited rapid oscillations in parallel experiments (Fig. 4 B; Video 2).
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Ca2+-triggered Ras deactivation by CAPRI monitored in live cells
In previous studies we were unable to reconstitute an in vitro GAP assay to monitor CAPRI function with temporal resolution (Lockyer et al., 2001). We therefore used the Ras-binding domain from Raf-1 tagged to GFP (GFP-RBD) as a reporter of active Ras on cell membranes (Walker and Lockyer, 2004). In a proportion of nonstarved cells cotransfected with H-Ras the RBD localized to the plasma membrane of HeLa cells (Fig. 5 A), or to the plasma membrane and Golgi apparatus of COS-7 cells (Fig. 5 B). By measuring the increase in cytosolic fluorescence as the RBD dissociated from the membrane (indicating Ras-GTP turnover), we determined the spatial and temporal kinetics of Ca2+-triggered CAPRI GAP activity in transfected cells in a similar approach to our recent analysis of RASAL (Walker et al., 2004).
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We analyzed the kinetics of GFP-RBD dissociation from the plasma membrane in CAPRI-expressing HeLa or COS-7 cells stimulated with histamine or ATP, respectively. COS-7 cells not transfected with CAPRI (Fig. 5 C), or cotransfected with CAPRI R473S (unpublished data), showed no dissociation of the RBD from the membrane. HeLa cells stimulated with histamine displayed a more sustained deactivation of Ras (Fig. 5 D) than COS-7 cells stimulated with ATP (Fig. 5 C). The data match the kinetics of GFP-CAPRI translocation by correlative experiments in Fig. 5 (E and F) or directly as in Fig. S3 (available at http://www.jcb.org/cgi/content/full/jcb.200504167/DC1). CAPRI has no detectable GAP activity at resting Ca2+, suggesting it exists in a closed conformation in the cytosol. We believe that conformational changes must occur to facilitate the activation of Ras GAP function upon translocation. Using GFP-RBD and H-Ras to transiently transfect cells has allowed us to follow the sustained Ca2+-triggered GAP activity of CAPRI in real time.
The fact that we can generate mini-oscillations of GFP-CAPRI is significant. It is likely that some GFP-CAPRI tracked cytosolic Ca2+ oscillations because membrane-binding sites were saturated. This indicates there is a threshold for plasma membrane ligand(s) that engages the PH domain. At higher intensity stimulation, excess ligand must be available to trap GFP-CAPRI, rendering the protein refractory to cytosolic Ca2+ fluctuations. Assuming that endogenous levels of CAPRI are lower than that achievable by overexpression, it seems highly unlikely that endogenous CAPRI oscillates during repetitive Ca2+ signals induced by G proteincoupled receptor activation because the membrane ligands for the PH domain would be in excess. This contrasts with RASAL, which is highly dependent on the C2 domains for membrane interactions according to our domain-swapping experiments. Thus, CAPRI is able to integrate the Ca2+-triggered translocation with a second signal provided by the PH domain. Each Ca2+-triggered GAP filters the Ca2+ signal differentially; this may convey alternative modes of information to regulate Ras and cell function.
Materials and methods
Constructs
GFP-CAPRI and deletion constructs were made as previously described (Lockyer et al., 2001). GFP-RASAL was made as previously described (Walker et al., 2004). GFP-RASAL HcRed-RBD was generated by PCR using GFP-RBD (human Raf-1 residues 51131) as a template with primers containing HindIII and BamHI overhangs for cloning into pHcRed-C1 (BD Biosciences). RFP-CAPRI was generated by replacing eGFP with mRFP by PCR using monomeric RFP template. GFP-CAPRI (W664A) was generated by PCR using the DpnI mutagenesis method with primers 5'-GAATGAGCTTAACCAGGCACT-3' and 5'-GCAAGCGCAGACAGTGCCTGGTTAAGCTCATTC-3'. GFP-CAPRI/RASAL was generated by PCR to produce a chimera of Met-1 to Leu-275 of CAPRI and Leu-274 to Pro-804 of RASAL. GFP-RASAL/CAPRI was generated by PCR to produce a chimera of Met-1 to Leu-273 of RASAL and Leu-276 to Thr-803 of CAPRI.
Live cell imaging
For Ca2+ imaging experiments, cells were loaded with 1 µM Fura-2 AM or 1 µM Fluo-4 AM (Molecular Probes, Inc.) in KH buffer (10 mM Hepes, 118 mM NaCl, 4.7 mM KCl, 10 mM glucose, 1.2 mM KH2PO4, 4.2 mM NaHCO3, 1.2 mM MgCl2, and 1.3 mM CaCl2, pH 7.4). Ca2+-free KH buffer omitted CaCl2 for 0.5 mM EGTA. Imaging was performed using a microscope (Diaphot; Nikon) and a Fluor 40x 1.3 NA oil immersion objective (Nikon). Fluorescence was collected using an LSR Rainbow filter wheel controller and an UltraPix 8-bit CCD camera (PerkinElmer). For sequential imaging of GFP/Fura-2 alternate excitation was at 380 nm and 490 nm (500 ms exposure). For ratio imaging of Fura-2loaded CHO cells excitation was 340 nm and 380 nm. The emission signal was collected at 510 nm and acquired in Imaging Suite Temporal Module V4.0 (3 x 3 binning, 0.3 frames/s; PerkinElmer). Ca2+ calibrations were made after determining Fmin and Fmax. Cells were maintained in KH buffer at 37°C using a heated jacket. Exchange of solutions was by bulk addition, e.g., 15 ml to a coverslip holder (2-ml volume) with vacuum line attachment.
All conventional confocal GFP imaging was performed on a PerkinElmer RS system (equipped with Yokogawa CSU 21 scanhead, Hamamatsu Orca ER camera and multi-line Argon laser 488 and 514 nm). Dual GFP/HcRed, dual GFP/mRFP, and Fluo-4/mRFP analysis was performed on a PerkinElmer LCI system (equipped with Yokogawa CSU 10 scanhead, Hamamatsu Orca ER camera, Sutter filter wheel, and multi-line Ar/Kr laser 488 and 568 nm). Each was attached to a microscope (Eclipse TE2000; Nikon) with Plan Fluor 40x 1.3 NA oil immersion objectives (Nikon). GFP excitation was at 488 nm and emission collected with a 500 LP filter at 2 x 2 binning, 0.25 frames/s. Images were acquired in RS Imaging Suite Temporal Module V1.0.0.6 (PerkinElmer). Sequential CFP and YFP imaging was performed on a confocal microscope (LSM 510 Meta; Carl Zeiss MicroImaging, Inc.). Fig. 5 A was processed in Adobe Photoshop version 7 as detailed in the figure legend. All other images were processed in CorelDraw version 11 for brightness, contrast, and intensity. The fluorescence intensity (F) of a region of interest in the cytosol, representing at least 10% of the total cytosolic surface area, was measured for each cell in a series of images. After background fluorescence was subtracted from the image series, the ratio of the fluorescence intensity at each time point (Ft) and the maximum fluorescence intensity of the same area (Fmax; usually at T = 0) was calculated. The Relative Translocation parameter = 1 Fmax/Ft. Relative Dissociation parameter = Fmax/Ft.
TIRFM was performed using a Biosystems cellR Imaging Station (Olympus) equipped with an MT20 illumination system, dual-port illuminator, Hamamatsu Orca ER camera, and 488 nm/532 nm solid-state lasers. This was attached to a microscope (Olympus) with a Plan Apo N 60x/1.45 NA oil TIRFM objective. Dual-port illumination enabled combined TIR and wide-field illumination for the fast and synchronized alternation of Fura-2 (380-nm nonratiometric measurement) and RFP imaging. The ratio of the fluorescence intensity at each time point (Ft) and the minimum fluorescence intensity of the same area (Fmin at T = 0) was calculated. By TIRFM the Relative Translocation parameter = 1 Fmin/Ft.
Online supplemental material
Details of cell culture and transfection are available online at http://www.jcb.org/cgi/content/full/jcb.200504167/DC1.
Acknowledgments
We thank M.R. Philips (New York University School of Medicine, New York, NY) for GFP-RBD and Ras constructs, T. Meyer (Stanford University, Stanford, CA) for GFP-PLC, and R. Tsien (University of California, San Diego, San Diego, CA) for mRFP.
This work was supported by the Biotechnology and Biological Sciences Research Council (Grant C20001 to P.J. Lockyer).
Submitted: 28 April 2005
Accepted: 8 June 2005
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