Mini-Review |
2 Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
Address correspondence to Vladimir I. Titorenko, Department of Biology, Concordia University, 7141 Sherbrooke St., West SP Building, Room SP-501-9, Montreal, Quebec H4B 1R6, Canada. Tel.: (514) 848-2424, ext. 3424. Fax: (514) 848-2881. email: VTITOR{at}vax2.concordia.ca; or Richard A. Rachubinski, Department of Cell Biology, University of Alberta, Medical Sciences Building 5-14, Edmonton, Alberta T6G 2H7, Canada. Tel.: (780) 492-9868. Fax: (780) 492-9278. email: rick.rachubinski{at}ualberta.ca
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Abstract |
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Recent studies have uncovered new and unexpected roles for organelles in cell proliferation, differentiation, death, and survival. Organelles generate and distribute signaling molecules and morphogens (González-Gaitán, 2003; Newmeyer and Ferguson-Miller, 2003; Scorrano et al., 2003), assemble active signaling protein complexes on their surface and transport them to specific subcellular locations (Sorkin and von Zastrow, 2002; Hancock, 2003), and serve as organizing platforms for the development of some viral and bacterial pathogens (Desjardins, 2003). A growing body of evidence supports the view that the peroxisome, an organelle known for its role in lipid metabolism and hydrogen peroxide detoxification (Titorenko and Rachubinski, 2001), is actively involved in orchestrating important developmental decisions from inside the cell. In humans, defects either in the biogenesis of the peroxisome or in its metabolic functions result in developmental disorders known as the peroxisomal disorders. These disorders are divided into two groups, the peroxisome biogenesis disorders (PBDs) and the peroxisome single enzyme disorders. The PBDs are due to autosomal recessive mutations in any of 12 PEX genes encoding proteins called peroxins (Gould et al., 2001; Matsumoto et al., 2003). A prominent feature of the Zellweger spectrum of the PBDs is a global developmental delay caused by the incomplete migration and differentiation of neuroblasts during psychomotor development, defects in the development of central white matter, and post-developmental enhanced apoptosis of neurons (Powers and Moser, 1998). Another distinct form of PBD, rhizomelic chondrodysplasia punctata type 1, is characterized by abnormal psychomotor development and mental retardation (Gould et al., 2001). Due to the extreme complexity of the human organism and its limited accessibility to genetic and biochemical analyses, the use of genetically and biochemically manipulable organisms with sequenced genomes as model systems with which to study the role of peroxisomes in development becomes increasingly important. Recent studies with such model systems have revealed various strategies and identified several mechanisms through which peroxisomes organize the processes of development, differentiation, and morphogenesis in evolutionarily distant organisms (Fig. 1).
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The peroxisome is an intracellular signaling compartment that promotes developmental decisions |
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The peroxisome compartmentalizes metabolic pathways essential for development and differentiation |
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In phytopathogenic fungi, acetyl-CoA derived from peroxisomal fatty acid ß-oxidation is used for the biosynthesis of melanin and glycerol (Thines et al., 2000; Kimura et al., 2001), which play pivotal roles in a distinct developmental program, the differentiation of asexual spores called conidia into an infectious structure, the appressorium (Kimura et al., 2001). Appressoria eventually penetrate the host plant, where they form infectious hyphae that invade the plant tissue. The inability to assemble peroxisomes due to a lack of the peroxisome-associated peroxin Pex6p, and the resultant deficiency in acetyl-CoA for the biosynthesis of melanin and glycerol, hamper phytopathogenicity (Kimura et al., 2001).
Impaired peroxisomal oxidation or transport of fatty acids, including very long-chain fatty acids, phytanic acid, and pristanic acid, in Zellweger patients leads to an accumulation of these toxic compounds outside the peroxisome and results in global developmental delay and severe neurological dysfunction (Gould et al., 2001). If not oxidized in peroxisomes, these abnormal fatty acids are incorporated into the membranes of neurons, perturbing their microenvironment and causing defects in their migration and differentiation, along with other developmental defects characteristic of PBD pathogenesis (Powers and Moser, 1998).
It appears that only a limited set of metabolic pathways and metabolite transporters operating in peroxisomes, rather than all the basic metabolic functions of these organelles, is required for certain developmental programs. Using RNA-mediated interference for the post-transcriptional silencing of numerous nematode genes controlling a wide range of peroxisome-related functions, Petriv et al. (2002) demonstrated that major metabolic pathways in the peroxisome, such as the ß-oxidation of various fatty acids, which supplies acetyl-CoA for energy production in mitochondria (Hettema and Tabak, 2000), are not required for post-embryonic development of the nematode. On the other hand, a defect in the peroxisomal transport of acyl-CoA esters of fatty acids, which results in their accumulation outside the peroxisome, and a deficiency in plasmalogen biosynthesis cause an arrest at the first larval stage of development (Petriv et al., 2002). Notably, in addition to post-embryonic development of the nematode, several other developmental programs in yeast (Titorenko et al., 1997), filamentous fungi (Jedd and Chua, 2000), plants (Footitt et al., 2002), and humans (Gavva et al., 2002) do not rely on the energy provided by the mitochondrial oxidation of acetyl-CoA derived from the peroxisomal ß-oxidation of fatty acids.
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Peroxisomal proteins possess dual subcellular localization and function |
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The human peroxin Pex14p is an integral peroxisomal membrane protein that functions as the initial docking site for cargo-laden cytosolic shuttling receptors of peroxisomal matrix proteins (Titorenko and Rachubinski, 2001). Human Pex14p is also a nuclear protein (Gavva et al., 2002). Targeted to the nucleus by its nuclear localization signal, human Pex14p interacts specifically with the p45 subunit of a DNA binding transcription factor, NF-E2. NF-E2 is a transcriptional regulator of erythroid and megakaryotic genes in pluripotential hematopoietic stem cells of the blood and lymphoid systems (Gavva et al., 2002). In the nucleus, a complex formed between Pex14p and a histone deacetylase is proposed to act as a corepressor of the NF-E2mediated transcription of these genes (Gavva et al., 2002). The involvement of a nuclear pool of human Pex14p in regulating gene transcription is still under debate.
The essential roles of three peroxisome-bound peroxins of the yeast Yarrowia lipolytica, Pex2p, Pex6p, and Pex16p, in peroxisome biogenesis are well established (Titorenko and Rachubinski, 2001). Y. lipolytica Pex2p and Pex16p are initially sorted to the ER and are then delivered in a Pex6p-dependent manner from the ER to peroxisomes via ER-derived vesicles (Titorenko et al., 1997; Titorenko and Rachubinski, 1998). Whereas the major portion of Pex2p, Pex6p, and Pex16p resides in peroxisomes, a minor fraction is ER associated (Titorenko and Rachubinski, 1998). While the peroxisome-bound pools of Pex2p, Pex6p, and Pex16p operate in peroxisome assembly and division, their ER-bound pools orchestrate a specific cell polarization and differentiation program, the dimorphic transition from a round yeast form to a filamentous (mycelial) form, by promoting the delivery of mycelium-specific proteins from the ER to the cell envelope (Titorenko et al., 1997; Titorenko and Rachubinski, 1998). The role for at least one peroxisome- and ER-localized bifunctional protein, Pex16p, in development and differentiation is evolutionarily conserved among yeasts and plants. The Arabidopsis orthologue of Y. lipolytica Pex16p, the SSE1 protein, is required not only for peroxisome assembly but also for the biogenesis of protein and lipid bodies, a cellular differentiation program for energy storage in maturing seeds (Lin et al., 1999). The biogenesis of protein and lipid bodies depends on the ER and involves the sorting of oleosins, the most abundant protein components of oil bodies, via the ER (Sarmiento et al., 1997). Importantly, Pex16p complements developmental defects in a cross-species fashion, as the Arabidopsis SSE1 protein reestablishes the dimorphic transition in the Y. lipolytica pex16 mutant deficient in the delivery of mycelium-specific proteins from the ER to the tip of the growing filament (Lin et al., 1999).
Another bifunctional peroxisomal membrane protein of plants, CTS, is not only involved in the transport of acyl-CoA esters of fatty acids (FA-CoAs) into the peroxisome but is also essential for the transition from dormancy to germination, an important step in the embryonic development of Arabidopsis (Footitt et al., 2002). Seeds carrying cts mutations do not germinate and exhibit a "forever dormant" phenotype. Importantly, the inability of cts mutants to metabolize lipid bodyderived FA-CoAs cannot account for the "forever dormant" phenotype, suggesting that the functions of CTS in the peroxisomal transport of FA-CoAs and in embryo development are different (Footitt et al., 2002). CTS is an orthologue of the human peroxisomal protein ALDP, a member of the ATP-binding cassette (ABC) transporter family. Because the binding of other known ABC transporters to certain passive ion channels modulates their development-related activities (Gaedeke et al., 2001), it has been proposed that the activity of CTS in embryo dormancy may involve its interaction with such channels (Footitt et al., 2002). It remains to be established what subcellular compartment serves as an organizing platform for the involvement of CTS in development. It is noteworthy that a human peroxisomal ABC transporter is present in a specialized subdomain of the ER from which peroxisomes form (Geuze et al., 2003). It is tempting to speculate that the ER-associated form of CTS promotes embryo development, while its peroxisomal form functions in the transport of FA-CoAs into this organelle. Experimental confirmation of this scenario awaits the detailed characterization of the protein composition and developmental dynamics of the peroxisomal ER subdomain that has been suggested to serve as a template for peroxisome formation (Titorenko and Rachubinski, 2001; Geuze et al., 2003).
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The peroxisome is a template for the formation of a distinct subcellular compartment essential for fungal development |
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The peroxisome is an intracellular platform for the development of some viral pathogens |
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Summary and perspectives |
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Acknowledgments |
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This work was supported in part by grants from the Canadian Institutes of Health Research to V.I. Titorenko. R.A. Rachubinski is an International Research Scholar of the Howard Hughes Medical Institute and Canada Research Chair in Cell Biology.
Submitted: 10 December 2003
Accepted: 14 January 2004
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