Correspondence to Marco Falasca: m.falasca{at}ucl.ac.uk
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Introduction |
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Recently, a growing interest has arisen in the members of class II PI3K and several lines of evidence suggest a potential role for these enzymes in agonist-mediated regulation of cellular functions (Foster et al., 2003). Such evidence includes activation of PI3K-C2 by insulin (Brown et al., 1999) and monocyte chemotactic peptide-1 (Turner et al., 1998) and through interaction with clathrin (Gaidarov et al., 2001). Similarly, insulin (Brown and Shepherd, 2001) and platelet aggregation (Zhang et al., 1998) have been reported to activate PI3K-C2ß. In addition, PI3K-C2
and PI3K-C2ß associate with polypeptide growth factor receptors (Arcaro et al., 2000) and recent data suggest that PI3K-C2ß is involved in EGF- and stem cell factordependent signals (Arcaro et al., 2002). Despite this evidence, a clear mechanism of activation and the precise intracellular roles of these enzymes are still not defined (Foster et al., 2003). Furthermore, there is currently no clear indication of their in vivo lipid products (Foster et al., 2003), although the observation that, in vitro, these enzymes display a strong substrate preference for phosphatidylinositol suggests phosphatidylinositol-3-phosphate (PtdIns-3-P) to be their main lipid product in vivo (Brown and Shepherd, 2001).
The role of PtdIns-3-P as a dynamic intracellular second messenger has been recently underscored by our work reporting that this phosphoinositide is generated upon insulin stimulation (Maffucci et al., 2003) and the demonstration that it plays a crucial role in insulin signaling (Chaussade et al., 2003; Maffucci et al., 2003). Evidence suggests that the insulin-dependent pool of PtdIns-3-P might be generated through activation of a class II PI3K enzyme (Maffucci et al., 2003).
Lysophosphatidic acid (LPA; 1-acyl-sn-glycerol-3-phosphate) is a serum-borne phospholipid that evokes a wide range of biological effects, including cell proliferation, migration, and survival (Moolenaar, 1994; Graler and Goetzl, 2002; Mills and Moolenaar, 2003; van Leeuwen et al., 2003). This large spectrum of biological activities is explained by the fact that LPA receptors can couple to three distinct G proteins, Gq, Gi, and G12/13, leading to activation of several intracellular messengers, including phospholipase C (PLC), the small GTPases RhoA and Rac, the RasMAPK cascade, and PI3K (Radeff-Huang et al., 2004). Two members of class I PI3K (p110ß and p110) have been reported to be involved in LPA signaling (Yart et al., 2002). In particular, p110
can be directly activated by G protein ß
subunits through association with the adaptor protein p101 (Stephens et al., 1997) and it has been implicated in the LPA-dependent RasMAPK activation (Takeda et al., 1999) and motility (Jung et al., 2004). Furthermore, it has been shown that at least in some cell lines the LPA-dependent accumulation of PI3K products is mediated by p110ß in a mechanism involving transactivation of the EGF receptor (Laffargue et al., 1999).
Among the different effects elicited by LPA, migration has recently received increasing attention (Gschwind et al., 2003; Stahle et al., 2003; van Leeuwen et al., 2003; Yamada et al., 2004). The importance of LPA in migration has been underscored by the discovery that autotaxin, a cell motility-stimulating ectophosphodiesterase involved in tumor invasion, can act as a lysophospholipase D and generate LPA from lysophosphatidylcholine in the extracellular environment (Umezu-Goto et al., 2002). Indeed, both LPA and autotaxin have been involved in motility of neoplastic and nonneoplastic cells (Hama et al., 2004). Cell migration is the result of different and well-coordinated physical processes and orchestrates embryonic morphogenesis, contributes to tissue repair and regeneration, and drives disease progression in cancer, atherosclerosis, and arthritis. Several lines of evidence suggest that 3-phosphoinositides play a role in directed migration via both G protein and tyrosine kinasemediated signaling processes. Here, we identify a novel pathway in LPA signaling that involves generation of the lipid second messenger PtdIns-3-P through activation of PI3K-C2ß and is crucial for LPA-dependent cell migration. This study is the first demonstration of an intracellular role for a class II PI3K and identification of two new important players in cell migration.
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Results |
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The LPA-dependent pool of PtdIns-3-P is generated at the plasma membrane
To localize the LPA-dependent pool of PtdIns-3-P, cells were transfected with a cDNA encoding GFP-2XFYVEHrs, a GFP fusion protein known to specifically bind PtdIns-3-P (Gillooly et al., 2000; Maffucci et al., 2003). Confocal microscopy analysis revealed that in COS7 (Fig. S1 D), HeLa (Fig. 2 A), and SKOV-3 (Fig. 2 B), LPA stimulation induced a rapid translocation of GFP-2XFYVEHrs to the plasma membrane that paralleled the time course of PtdIns-3-P formation observed by HPLC analysis (for COS7 compare Fig. S1 B and histogram in Fig. S1 D; for SKOV-3 compare Fig. 1 B and Fig. S2 B). As shown in Fig. 2 C, plasma membrane translocation in SKOV-3 was confirmed by colocalization of YFP-2XFYVEHrs and the plasma membrane marker CFP-PH PLC1 (Maffucci et al., 2003).
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Effect of PtdIns-3-Pspecific phosphatase.
These observations further prompted us to specifically check a potential role for PtdIns-3-P in LPA-dependent migration. Therefore, we analyzed migration of SKOV-3 transfected with a cDNA encoding a Flag-tagged PtdIns-3-Pspecific phosphatase, myotubularin MTM1 (Laporte et al., 2002), that has been recently successfully used to inhibit the insulin-dependent pool of PtdIns-3-P (Chaussade et al., 2003). In these experiments, migration was assessed in Transwell assays by counting the total number of migrated cells as described in Materials and methods. Although the transfection efficiency in SKOV-3 was only 50%, overexpression of MTM1 was able to reduce the LPA-induced migration whereas overexpression of the point mutant MTM1 C375S, which has no phosphatase activity toward PtdIns-3-P, had no effect (Fig. 6 A, top). Expression of both proteins was assessed in Western blotting analysis (Fig. 6 A, bottom). Although expression of MTM1 was able to induce a partial inhibition of migration, it did not seem to affect the levels of PtdIns-3,4,5-P3 as basal Akt phosphorylation was not even partially reduced (Fig. 6 B). Importantly, the lack of effect on Akt phosphorylation was not due to the transfection efficiency because overexpression of the PtdIns-3,4,5-P3specific phosphatase PTEN at similar transfection efficiency was indeed able to reduce Akt phosphorylation (Fig. 6 C).
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To clearly assess the effect of the PtdIns-3-Psequestering domain, we performed a very accurate second set of experiments and counted only the transfected (green) cells that had migrated. Indeed, in this more precise assay we observed that migration was completely inhibited in cells expressing GFP-2XFYVEHrs, whereas cells expressing GFP alone migrated upon LPA stimulation (Fig. 6 F). As discussed in the previous section, although a 50% transfection efficiency of GFP-PTEN was enough to reduce Akt phosphorylation, basal levels of Akt phosphorylation were not even partially reduced in cells transfected with GFP-2XFYVEHrs with the same efficiency (Fig. 6 G).
When we performed similar experiments in HeLa we observed a total inhibition of the LPA-mediated migration in cells expressing GFP-2XFYVEHrs, a finding consistent with the high transfection efficiency of these cells (>80%; Fig. 6, H and I; and Fig. S3 E). To further confirm that inhibition of migration was due to a specific sequestering of PtdIns-3-P, we transfected HeLa with a cDNA encoding another fusion protein also known to bind PtdIns-3-P (GFP-PX p40phox) that we have already used to monitor the intracellular PtdIns-3-P (Maffucci et al., 2003). As a control we used a mutant of this domain with a reduced ability to bind PtdIns-3-P (GFP-PX p40phox Y59A/L65Q). GFP-PX p40phox inhibited the LPA-mediated migration, whereas less effect was observed in cells transfected with the mutated version of the protein (Fig. 6, H and I). Similar results were obtained in Transwell assay (Fig. S3 E). Overexpression of these domains did not affect cell growth (Fig. S3 F).
Together these data indicate that the lipid second messenger PtdIns-3-P is a new effector in LPA cascade and plays a role in the LPA-mediated cell migration.
The LPA-dependent pool of PtdIns-3-P is generated through PI3K-C2ß activation
The observation that in HeLa and SKOV-3 LPA did not affect the levels of PtdIns-3,4,5-P3, the major in vivo product of class I PI3Ks, together with the observed resistance to the inhibitor LY294002 ruled out the possibility that PtdIns-3-P derives from activation of the class I PI3Ks. This finding is in agreement with recent data indicating that the LPA-dependent migration of SKOV-3 requires activation of a RasMEK kinase 1 pathway but not class I PI3K (Bian et al., 2004). Several lines of evidence suggest that, at least in insulin signaling, the stimulated pool of PtdIns-3-P is the product of a class II PI3K (Maffucci et al., 2003). Moreover, the high sensitivity to wortmannin and reduced sensitivity to LY294002 observed in this study was in agreement with observed in vitro sensitivities of the class II enzyme PI3K-C2ß to these inhibitors and is distinct from that observed for class I enzymes (Arcaro et al., 1998; Fig. S4, A and B, available at http://www.jcb.org/cgi/content/full/jcb.200408005/DC1). On the basis of these observations, we hypothesized that the LPA-dependent pool of PtdIns-3-P might be generated through activation of a class II PI3K.
To investigate this hypothesis, we examined the effect of specific inhibition of these isoforms on the LPA-dependent generation of PtdIns-3-P at the plasma membrane. As there are no class II PI3K-specific chemical inhibitors available, we used RNA interference to specifically knockdown the class II isoforms PI3K-C2ß and PI3K-C2. Then, we analyzed the intracellular localization of GFP-2XFYVEHrs in LPA-stimulated SKOV-3 transfected with the corresponding siRNAs. A complete inhibition of LPA-dependent GFP-2XFYVEHrs plasma membrane translocation was observed in SKOV-3 transfected with the PI3K-C2ß siRNA. No inhibition was observed in cells transfected with the PI3K-C2
siRNA (Fig. 7, A and B). The observed complete inhibition was consistent with a high uptake of siRNAs in SKOV-3 that was confirmed by Western blotting analysis showing that a >90% knockdown of protein expression was achieved (Fig. 7 C). The higher transfection efficiency compared with plasmid DNAs is in agreement with reported high uptake of siRNAs in cells (Holen et al., 2002; Splinter et al., 2003). The LPA-dependent activation of PI3K-C2ß was confirmed by in vitro lipid kinase assay (Fig. 8 A). In the same experiments, no activation of PI3K-C2
was observed (unpublished data). In addition, we observed that LPA induced the translocation of a myc-tagged version of PI3K-C2ß to the plasma membrane in both HeLa (Fig. 8 B) and SKOV-3 (Fig. 8 C), suggesting that formation of PtdIns-3-P might occur through an LPA-dependent targeting and subsequent activation of the enzyme at the plasma membrane.
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Discussion |
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There is currently a growing interest in class II PI3Ks because several lines of evidence suggest that different agonists can activate these enzymes; however, their precise intracellular role and their lipid products in vivo still remain to be established (Foster et al., 2003). Here, we show that LPA activates PI3K-C2ß and that knockdown of this enzyme specifically inhibits formation of PtdIns-3-P at the plasma membrane upon LPA stimulation. This is the first paper showing that PI3K-C2ß is activated and PtdIns-3-P is generated upon LPA stimulation, and therefore, this work identifies a novel PI3K-C2ßPtdIns-3-P pathway in LPA signaling. Furthermore, this study is the first clear indication that PtdIns-3-P might be the in vivo product of PI3K-C2ß.
The PI3K-C2ßPtdIns-3-P pathway is involved in the LPA-dependent cell migration
The observation that the sensitivity to PI3K inhibitors of LPA-dependent migration is very similar to the effect observed on PtdIns-3-P formation led us to hypothesize that this pathway might be involved in the LPA-dependent cell migration. As for PtdIns-3-P formation, treatment with 100 nM wortmannin blocked migration whereas 1 µM LY294002 had no effect and 10 µM LY294002 had a partial effect on the LPA-mediated migration. Sensitivity to LY294002 is a key feature of PI3K-C2ß that shows a different sensitivity to this inhibitor compared with class I PI3K (Fig. S4 B). In particular, 1 µM LY294002, although able to significantly inhibit class I PI3K, has no effect on the activity of PI3K-C2ß. More crucially, a concentration of 10 µM LY294002 completely blocks class I PI3K, whereas it has only a partial inhibitory effect on PI3K-C2ß. It is important to underline that 10 µM LY294002 represents a crucial point in the curve of sensitivity of PI3K-C2ß that is where the activity of the enzyme significantly drops (Fig. S4 B). The observation that 10 µM LY294002 corresponds to the slope in the curve of sensitivity of PI3K-C2ß might explain the variability in the results that in some experiments was associated with the use of such concentration of the inhibitor (e.g., see the high SEM associated with the PtdIns-3-P formation in Fig. 3 A). However, even taking into account such variability, it is clear that both the LPA-dependent generation of PtdIns-3-P and migration are quite resistant to treatment with LY294002, and this finding is consistent with the observed sensitivity of PI3K-C2ß.
Specific blockade of PtdIns-3-P (by overexpression of either a PtdIns-3-Pspecific phosphatase or PtdIns-3-Pbinding domains) blocks the LPA-dependent migration. In SKOV-3, the observed effects were partial when we counted the total number of migrated cells, and this result is consistent with a partial transfection efficiency of plasmid DNAs in SKOV-3 (50%). However, when we monitored migration only in transfected (green) SKOV-3, we observed a complete inhibition of migration. Similarly, inhibition of migration was complete in HeLa whose transfection efficiency was >80%. Additionally, knockdown of PI3K-C2ß in both cell lines inhibits the LPA-mediated migration. Together, these data indicate that this newly identified PI3K-C2ßPtdIns-3-P signaling pathway is necessary for the LPA-dependent cell migration. These data represent the first clear identification of an intracellular role for a member of class II PI3K and they may shed new light on PI3K-mediated intracellular events.
Over the last decade wide interest has been focused on understanding the mechanisms underlying the major steps of cell migration and the signaling pathways regulating them. On the one hand, cell migration plays a crucial role in a variety of biological processes such as morphogenesis, inflammation, and wound healing. On the other hand, migration is involved in several diseases, including cancer metastasis, atherosclerosis, and arthritis. Therefore, the identification of both PI3K-C2ß and PtdIns-3-P as important players in cell migration may help clarify both physiological and pathological mechanisms of cell migration and represent a novel target for therapeutic intervention. Several lines of evidence are now revealing the crucial role for LPA as a motility factor. It has been shown that in human pancreatic carcinoma cell lines LPA stimulates cell migration but not proliferation (Stahle et al., 2003). More interestingly, LPA has been identified as a critical component of ascites for the motility of pancreatic cancer cells (Yamada et al., 2004). In addition, it has been reported that the ectoenzyme autotaxin, a cell motility-stimulating ectophosphodiesterase, can generate LPA in the extracellular environment (Umezu-Goto et al., 2002), and both LPA and autotaxin are involved in motility of neoplastic and nonneoplastic cells (Hama et al., 2004). These data, together with the observation that elevated plasma LPA concentrations have been found in patients with ovarian cancer compared with healthy control group (Xu et al., 1995; Westermann et al., 1998) and that prostate cancer cells produce high levels of LPA (Xie et al., 2002), definitively support a role for LPA in the initiation or progression of malignant disease (Mills and Moolenaar, 2003).
The discovery of this novel PI3K-C2ßPtdIns-3-P pathway in LPA-dependent migration of different cell lines, including an ovarian cancer cell line, may therefore result useful in elucidating the role of LPA in this disease. Furthermore, because a wide range of mammalian cell types are LPA-responsive, it is tempting to speculate that the PI3K-C2ßPtdIns-3-P pathway might have a more general role in different biological contexts.
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Materials and methods |
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Cell cultures and transfection
Cells were maintained in DME supplemented with 10% FBS and transfected with LipofectAMINE PLUS (Invitrogen) according to the manufacturer. Transfection with siRNAs was performed as described previously (Elbashir et al., 2002) and cells were used after 4872 h.
HPLC analysis
COS7, HeLa, and SKOV-3 were incubated with 10 µCi/well myo-[3H]inositol (PerkinElmer) in inositol-free medium M199 for 24 h, and then left untreated or stimulated with LPA for different times. HPLC analysis was performed as described previously (Maffucci et al., 2003).
TLC analysis
Serum-starved cells were incubated with 0.8 mCi/well [32P]phosphoric acid (PerkinElmer) in phosphate-free medium, M3786, for 3 h before stimulation as indicated. Phospholipids were extracted, dried under nitrogen, resuspended in 100 µl chloroform/methanol (3:2), and spotted onto silica plates presoaked in water/methanol (3:2) and 1.2% (wt/vol) oxalic acid, and oven baked for 15 min at 110°C. TLC plates were developed in chloroform/acetone/methanol/acetic acid/water (90:36:30:27:18).
Confocal microscopy and quantitative analysis
Cells grown on glass coverslips were fixed with PBS containing 4% PFA for 10 min at RT. Where necessary, cells were permeabilized with 0.25% Triton X-100 and incubated with an anti-myc antibody followed by an FITC secondary antibody. Microscopy was performed by using a laser confocal microscope system (TCS NT; Leica) connected to a microscope (model DMXRE; Leica) equipped with a 63x water immersion objective. Images were acquired and loaded by using a TCS NT program (version 1.6.587). No further processing of the images was done except for changes in brightness/contrast to better visualize the data. For quantitative analyses of GFP-2XFYVEHrs translocation, GFP-2XFYVEHrs-transfected cells were serum starved for 24 h and stimulated with LPA for the indicated times before fixing for confocal microscopy. At least 100 cells/coverslip were analyzed by blind scoring (no score: cells with 025% membrane localization; score 1: cells with >25% membrane localization). The number of cells showing a plasma membrane localization of the GFP-2XFYVEHrs was expressed as a percentage of control. Statistical analyses were performed by paired t test.
PI3K assay
PI3K activity assay was performed as described previously (Kamalati et al., 2000).
Migration assays
Wound healing assay.
Confluent cells were serum deprived overnight, left untreated or treated as indicated, and then wounded with a linear scratch by a sterile pipette tip. After washing, cells were incubated in the presence or absence of LPA and 0.5 µg/ml mitomycin-C. Time-lapse microscopy was performed by using a microscope (model Eclipse TE2000-U; Nikon) equipped with a temperature controller (Solvent Scientific; 37°C). No further processing of the images was done except for changes in brightness/contrast to better visualize the data. Cell movement was recorded with a charge-coupled device camera (OrcaER; Hamamatsu Photonics) by using PCI Software (Digitalpixel). To obtain the quantitative analyses of Fig. 4 B and Fig. 5 B, panels as those shown in Fig. 4 A and Fig. 5 A were prepared for each experiment. Each panel was made up of images of cells collected at time 0 and every hour (117 h) for each condition (control, LPA, and different concentrations of PI3K inhibitors+LPA). The wounded area was defined in each image by positioning red lines in correspondence of the original scratch. The number of cells migrated into the wounded area at different hours was visually counted. Alternatively, the wounded cell monolayers were photographed using a microscope (Nikon). For quantitative analysis shown in Fig. 6 I, experiments were performed in cell culture dishes with a 2-mm grid (Nunc) and migration was quantified by counting the number of cells per grid square (4 mm2) by using a microscope (Nikon).
Transwell assay.
Alternatively, cell migration was performed in Transwell chambers (tissue culture treated, 10-mm-diam, 8 µm pores; Nunc) coated with 100 µg/ml of type I collagen. Cells were resuspended in DME containing 1% BSA, added (30,000 cells/150 µl) to the top of each migration chamber, and allowed to migrate in the presence of LPA in the lower chamber. After 4 h, cells that had not migrated were removed using a cotton swab, whereas migrated cells were fixed with 4% PFA. To count the total number of migrated cells, the membranes of the Transwell chambers were stained with 1% crystal violet and analyzed by phase-contrast microscopy using a microscope (Nikon; examples are shown for HeLa in Fig. 9 D). To count only the transfected cells, we took advantage of the observation that they expressed GFP-tagged proteins. Therefore, the Transwell chambers were analyzed by fluorescent microscopy using a microscope (model DMRXE; Leica) equipped with a 10x objective. In this way, only fluorescent (green) cells that had migrated were visualized and counted.
Cell growth
104 cells/well in a 48-well plate were serum deprived overnight before adding LPA for a further 48 h. [3H]thymidine (0.5 µCi/well) was added for the last 18 h. After washing with ice-cold PBS, wells were incubated with 5% TCA at +4°C for 20 min, washed with PBS and methanol, and air dried. After 30 min in 0.2 M NaOH, samples were collected and radioactivity was assessed by scintillation counting. Alternatively, MTT assay was performed as described previously (Piccolo et al., 2004).
Phospholipase C activation
Cells were incubated with 2 µCi/well myo-[3H]inositol (PerkinElmer) in M199. After 24 h, cells were incubated in M199 plus 10 mM Hepes and 20 mM LiCl for 15 min before stimulation, scraped in 0.1 M HCOOH, and the total amount of inositol phosphates was assessed using AG 1-X8 resin (formate form, 200400 mesh; Bio-Rad Laboratories) and liquid radioactivity counting.
Online supplemental material
Fig. S1, Fig. S2, Fig. S3, Fig. S4 and the corresponding legends are available in the online supplemental material. Online supplemental material is available at http://www.jcb.org/cgi/content/full/jcb.200408005/DC1.
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Acknowledgments |
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This work was supported by the Association for International Cancer Research (grant 05-127 to M. Falasca), Compagnia di San Paolo (to M. Falasca), and Diabetes UK (grant BDA:RD02/0002388 to M. Falasca). T. Maffucci is the recipient of a Diabetes UK RD Lawrence Fellowship (grant BDA:RD04/0002884). F.T. Cooke, F.M. Foster, and C.J. Traer are supported by the Wellcome Trust, the Biotechnology and Biological Sciences Research Council (grant 4S/C14421 to M.J. Fry), and a Research Endowment Trust Funds studentship from the University of Reading, respectively. M. Falasca is supported by an endowment from the Dr. Mortimer and Mrs. Theresa Sackler Trust.
The authors declare that there are no conflicts of interest regarding this article.
Submitted: 2 August 2004
Accepted: 14 April 2005
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References |
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![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Anderson, N.G., T. Ahmad, K. Chan, R. Dobson, and N.J. Bundred. 2001. ZD1839 (Iressa), a novel epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor, potently inhibits the growth of EGFR-positive cancer cell lines with or without erbB2 overexpression. Int. J. Cancer. 94:774782.[CrossRef][Medline]
Arcaro, A., S. Volinia, M.J. Zvelebil, R. Stein, S.J. Watton, M.J. Layton, I. Gout, K. Ahmadi, J. Downward, and M.D. Waterfield. 1998. Human phosphoinositide 3-kinase C2beta, the role of calcium and the C2 domain in enzyme activity. J. Biol. Chem. 273:3308233090.
Arcaro, A., M.J. Zvelebil, C. Wallasch, A. Ullrich, M.D. Waterfield, and J. Domin. 2000. Class II phosphoinositide 3-kinases are downstream targets of activated polypeptide growth factor receptors. Mol. Cell. Biol. 20:38173830.
Arcaro, A., U.K. Khanzada, B. Vanhaesebroeck, T.D. Tetley, M.D. Waterfield, and M.J. Seckl. 2002. Two distinct phosphoinositide 3-kinases mediate polypeptide growth factor-stimulated PKB activation. EMBO J. 21:50975108.
Bian, D., S. Su, C. Mahanivong, R.K. Cheng, Q. Han, Z.K. Pan, P. Sun, and S. Huang. 2004. Lysophosphatidic acid stimulates ovarian cancer cell migration via a Ras-MEK Kinase 1 pathway. Cancer Res. 64:42094217.
Brown, R.A., and P.R. Shepherd. 2001. Growth factor regulation of the novel class II phosphoinositide 3-kinases. Biochem. Soc. Trans. 29:535537.[CrossRef][Medline]
Brown, R.A., J. Domin, A. Arcaro, M.D. Waterfield, and P.R. Shepherd. 1999. Insulin activates the alpha isoform of class II phosphoinositide 3-kinase. J. Biol. Chem. 274:1452914532.
Cantley, L.C. 2002. The phosphoinositide 3-kinase pathway. Science. 296:16551657.
Chaussade, C., L. Pirola, S. Bonnafous, F. Blondeau, S. Brenz-Verca, H. Tronchere, F. Portis, S. Rusconi, B. Payrastre, J. Laporte, and E. Van Obberghen. 2003. Expression of myotubularin by an adenoviral vector demonstrates its function as a phosphatidylinositol 3-phosphate [PtdIns(3)P] phosphatase in muscle cell lines: involvement of PtdIns(3)P in insulin-stimulated glucose transport. Mol. Endocrinol. 17:24482460.
Elbashir, S.M., J. Harborth, K. Weber, and T. Tuschl. 2002. Analysis of gene function in somatic mammalian cells using small interfering RNAs. Methods. 26:199213.[CrossRef][Medline]
Foster, F.M., C.J. Traer, S.M. Abraham, and M.J. Fry. 2003. The phosphoinositide (PI) 3-kinase family. J. Cell Sci. 116:30373040.
Gaidarov, I., M.E. Smith, J. Domin, and J.H. Keen. 2001. The class II phosphoinositide 3-kinase C2alpha is activated by clathrin and regulates clathrin-mediated membrane trafficking. Mol. Cell. 7:443449.[CrossRef][Medline]
Gillooly, D.J., I.C. Morrow, M. Lindsay, R. Gould, N.J. Bryant, J.M. Gaullier, R.G. Parton, and H. Stenmark. 2000. Localization of phosphatidylinositol 3-phosphate in yeast and mammalian cells. EMBO J. 19:45774588.
Graler, M.H., and E.J. Goetzl. 2002. Lysophospholipids and their G protein-coupled receptors in inflammation and immunity. Biochim. Biophys. Acta. 1582:168174.[Medline]
Gschwind, A., S. Hart, O.M. Fischer, and A. Ullrich. 2003. TACE cleavage of proamphiregulin regulates GPCR-induced proliferation and motility of cancer cells. EMBO J. 22:24112421.
Hama, K., J. Aoki, M. Fukaya, Y. Kishi, T. Sakai, R. Suzuki, H. Ohta, T. Yamori, M. Watanabe, J. Chun, and H. Arai. 2004. Lysophosphatidic acid and autotaxin stimulate cell motility of neoplastic and non-neoplastic cells through LPA1. J. Biol. Chem. 279:1763417639.
Holen, T., M. Amarzguioui, M. Wiiger, E. Babaie, and H. Prydz. 2002. Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor. Nucleic Acids Res. 30:17571766.
Jung, I.D., J. Lee, K.B. Lee, C.G. Park, Y.K. Kim, D.W. Seo, D. Park, H.W. Lee, J.W. Han, and H.Y. Lee. 2004. Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells. Eur. J. Biochem. 271:15571565.
Kamalati, T., H.E. Jolin, M.J. Fry, and M.R. Crompton. 2000. Expression of the BRK tyrosine kinase in mammary epithelial cells enhances the coupling of EGF signaling to PI 3-kinase and Akt, via erbB3 phosphorylation. Oncogene. 19:54715476.[CrossRef][Medline]
Katso, R., K. Okkenhaug, K. Ahmadi, S. White, J. Timms, and M.D. Waterfield. 2001. Cellular function of phosphoinositide 3-kinases: implications for development, homeostasis and cancer. Annu. Rev. Cell Dev. Biol. 17:615675.[CrossRef][Medline]
Laffargue, M., P. Raynal, A. Yart, C. Peres, R. Wetzker, S. Roche, B. Payrastre, and H. Chap. 1999. An epidermal growth factor receptor/Gab1 signaling pathway is required for activation of phosphoinositide 3-kinase by lysophosphatidic acid. J. Biol. Chem. 274:3283532841.
Laporte, J., F. Blondeau, A. Gansmuller, Y. Lutz, and J.-L. Vonesch. 2002. The PtdIns3P phosphatase myotubularin is a cytoplasmic protein that also localizes to Rac1-inducible plasma membrane ruffles. J. Cell Sci. 115:31053117.
Maffucci, T., A. Brancaccio, E. Piccolo, R.C. Stein, and M. Falasca. 2003. Insulin induces phosphatidylinositol-3-phosphate formation through TC10 activation. EMBO J. 22:41784189.
Mills, G.B., and W.H. Moolenaar. 2003. The emerging role of lysophosphatidic acid in cancer. Nat. Rev. Cancer. 3:582591.[CrossRef][Medline]
Moolenaar, W.H. 1994. LPA: a novel lipid mediator with diverse biological actions. Trends Cell Biol. 4:213219.[CrossRef][Medline]
Piccolo, E., S. Vignati, T. Maffucci, P.F. Innominato, A.M. Riley, B.V. Potter, P.P. Pandolfi, M. Broggini, S. Iacobelli, P. Innocenti, and M. Falasca. 2004. Inositol pentakisphosphate promotes apoptosis through a PI 3-K/Akt pathway. Oncogene. 23:17541765.[CrossRef][Medline]
Radeff-Huang, J., T.M. Seasholtz, R.G. Matteo, and J.H. Brown. 2004. G protein mediated signaling pathways in lysophospholipid induced cell proliferation and survival. J. Cell. Biochem. 92:949966.[CrossRef][Medline]
Rameh, L.E., and L.C. Cantley. 1999. The role of phosphoinositide 3-kinase lipid products in cell function. J. Biol. Chem. 274:83478350.
Razzini, G., A. Brancaccio, M.A. Lemmon, S. Guarnieri, and M. Falasca. 2000. The role of the pleckstrin homology domain in membrane targeting and activation of phospholipase Cbeta(1). J. Biol. Chem. 275:1487314881.
Splinter, P., A.I. Masyuk, and N.F. LaRusso. 2003. Specific inhibition of AQP1 water channels in isolated rat intrahepatic bile duct units by small interfering RNAs. J. Biol. Chem. 278:62686274.
Stahle, M., C. Veit, U. Bachfischer, K. Schierling, B. Skripczynski, A. Hall, P. Gierschik, and K. Giehl. 2003. Mechanisms in LPA-induced tumor cell migration: critical role of phosphorylated ERK. J. Cell Sci. 116:38353846.
Stephens, L.R., A. Eguinoa, H. Erdjument-Bromage, M. Lui, F. Cooke, J. Coadwell, A.S. Smrcka, M. Thelen, K. Cadwallader, P. Tempst, and P.T. Hawkins. 1997. The G beta gamma sensitivity of a PI3K is dependent upon a tightly associated adaptor, p101. Cell. 89:105114.[CrossRef][Medline]
Takeda, H., T. Matozaki, T. Takada, T. Noguchi, T. Yamao, M. Tsuda, F. Ochi, K. Fukunaga, K. Inagaki, and M. Kasuga. 1999. PI 3-kinase gamma and protein kinase C-zeta mediate RAS-independent activation of MAP kinase by a Gi protein-coupled receptor. EMBO J. 18:386395.
Turner, S.J., J. Domin, M.D. Waterfield, S.G. Ward, and J. Westwick. 1998. The CC chemokine monocyte chemotactic peptide-1 activates both the class I p85/p110 phosphatidylinositol 3-kinase and the class II PI3K-C2alpha. J. Biol. Chem. 273:2598725995.
Umezu-Goto, M., Y. Kishi, A. Taira, K. Hama, N. Dohmae, K. Takio, T. Yamori, G.B. Mills, K. Inoue, J. Aoki, and H. Arai. 2002. Autotaxin has lysophospholipase D activity leading to tumor cell growth and motility by lysophosphatidic acid production. J. Cell Biol. 158:227233.
van Leeuwen, F.N., B.N. Giepmans, L.A. van Meeteren, and W.H. Moolenaar. 2003. Lysophosphatidic acid: mitogen and motility factor. Biochem. Soc. Trans. 31:12091212.[Medline]
Westermann, A.M., E. Havik, F.R. Postma, J.H. Beijnen, O. Dalesio, W.H. Moolenaar, and S. Rodenhuis. 1998. Malignant effusions contain lysophosphatidic acid (LPA)-like activity. Ann. Oncol. 9:437442.[Abstract]
Xie, Y., T.C. Gibbs, Y.V. Mukhin, and K.E. Meier. 2002. Role for 18:1 lysophosphatidic acid as an autocrine mediator in prostate cancer cells. J. Biol. Chem. 277:3251632526.
Xu, Y., D.C. Gaudette, J.D. Boynton, A. Frankel, X.J. Fang, A. Sharma, J. Hurteau, G. Casey, A. Goodbody, A. Mellors, et al. 1995. Characterization of an ovarian cancer activating factor in ascites from ovarian cancer patients. Clin. Cancer Res. 1:12231232.[Abstract]
Yamada, T., K. Sato, M. Komachi, E. Malchinkhuu, M. Tobo, T. Kimura, A. Kuwabara, Y. Yanagita, T. Ikeya, Y. Tanahashi, et al. 2004. Lysophosphatidic acid (LPA) in malignant ascites stimulates motility of human pancreatic cancer cells through LPA1. J. Biol. Chem. 279:65956605.
Yart, A., H. Chap, and P. Raynal. 2002. Phosphoinositide 3-kinases in lysophosphatidic acid signaling: regulation and cross-talk with the Ras/mitogen-activated protein kinase pathway. Biochim. Biophys. Acta. 1582:107111.[Medline]
Zhang, J., H. Banfic, F. Straforini, L. Tosi, S. Volinia, and S.E. Rittenhouse. 1998. A type II phosphoinositide 3-kinase is stimulated via activated integrin in platelets. A source of phosphatidylinositol 3-phosphate. J. Biol. Chem. 273:1408114084.