Discrimination of ATP, ADP, and AMPPNP by Chaperonin GroEL

HEXOKINASE TREATMENT REVEALED THE EXCLUSIVE ROLE OF ATP*

Fumihiro Motojima and Masasuke Yoshida {ddagger}

From the Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan

Received for publication, January 24, 2003 , and in revised form, May 7, 2003.


    ABSTRACT
 TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS AND DISCUSSION
 REFERENCES
 
The double ring chaperonin GroEL binds unfolded protein, ATP, and GroES to the same ring, generating the cis ternary complex in which folding occurs within the cavity capped by GroES (cis folding). The functional role of ATP, however, remains unclear since several reports have indicated that ADP and AMPPNP (5'-adenylyl-{beta},{gamma}-imidodiphosphate) are also able to support the formation of the cis ternary complex and the cis folding. To minimize the effect of contaminated ATP and adenylate kinase, we have included hexokinase plus glucose in the reaction mixtures and obtained new results. In ADP and AMPPNP, GroES bound quickly to GroEL but bound very slowly to the GroEL loaded with unfolded rhodanese or malate dehydrogenase. ADP was unable to support the formation of cis ternary complex and cis folding. AMPPNP supported cis folding of malate dehydrogenase to some extent but not cis folding of rhodanese. In the absence of hexokinase, apparent cis folding of rhodanese and malate dehydrogenase was observed in ADP and AMPPNP. Thus, the exclusive role of ATP in generation of the cis ternary complex is now evident.


    INTRODUCTION
 TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS AND DISCUSSION
 REFERENCES
 
The bacterial chaperonin system consisting of GroEL and GroES facilitates folding of other proteins using the energy of ATP hydrolysis. GroEL is composed of 14 identical 57-kDa subunits, each containing a site for binding and hydrolysis of ATP. Seven GroEL subunits are arranged in a heptamer ring forming a central cavity, and two heptamer rings are stacked back to back. GroES is a dome-shaped, single heptamer ring of 10-kDa subunits. GroEL binds a wide range of unfolded proteins at the apical cavity surface and subsequently binds ATP and GroES to the same GroEL ring (the cis ring, a GroEL heptamer ring that binds to GroES), producing the complex consisting of GroEL, unfolded protein, and GroES (the cis ternary complex). Since the residues of the GroEL apical surface involved in GroES binding are mostly overlapped with substrate protein binding (1), GroES binding results in encapsulating unfolded protein into the enlarged cavity of the cis GroEL ring capped by GroES (the cis cavity) (2). The unfolded protein initiates folding in the cis cavity without a risk of aggregation (the cis folding). ATP hydrolysis in the cis ring and subsequent ATP binding to the opposite side of GroEL ring (the trans ring) induce the release of GroES, ADP, and substrate protein (whether folded or not) from the cis ring (3, 4). When unfolded protein is added to the GroEL-GroES complex, it binds to the trans ring, and its folding is arrested (2). Binding and release from the trans ring enable the folding for some proteins (2), especially large ones that are too large to be encapsulated in the cis cavity, by lowering the concentration of aggregation-prone folding intermediates in bulk solution (5). In contrast, the stringent substrate proteins for chaperonin fold efficiently by the cis folding in the presence of ATP (3, 6).

However, it should be noted that the functional significance of ATP for the cis ternary complex formation is unclear yet. It was reported that slow cis folding of rhodanese, a stringent substrate protein, is observed when GroES and ADP or AMPPNP1 were added to GroEL-unfolded rhodanese complex (2, 7, 8). The cis folding of dehydrofolate reductase in the presence of ADP was also reported (9). Malate dehydrogenase (MDH) and Rubisco were shown to form the cis ternary complex in ADP. However, these complexes did not promote folding (3). These observations have raised an intriguing question: what is the difference between the non-productive ADP-induced cis ternary complex and the productive ATP-induced cis ternary complex?

As reported previously (10), we have noticed that inclusion of hexokinase and glucose in the reaction mixtures diminished ADP-dependent cis folding of green fluorescent protein. Commercially prepared ADP and AMPPNP usually contain a trace amount of ATP. Also, if a trace amount of adenylate kinase is contaminated in purified protein, it can produce ATP from ADP constantly. Hexokinase would eliminate these unwanted ATPs during the reaction period. Another potential factor that can affect the results is heterogeneity of GroEL complexes in the reaction mixtures. For example, if two GroEL rings are not saturated by unfolded proteins and if GroES can bind preferably to free GroEL rings rather than the unfolded protein-loaded GroEL rings, two kinds of complexes would be generated: GroEL-unfolded protein complex without GroES or GroEL-GroES complex without unfolded protein in the cis cavity. Since these two complexes cannot be separated, the results may be taken as an evidence for the existence of the GroEL-GroES-unfolded protein ternary complex. Here, we reexamined the nucleotide requirement for the cis ternary complex by taking the precautions described above. The results showed that ADP is incompetent to generate the cis ternary complex, that is, ATP is stringent for the fast GroES binding to the unfolded protein-loaded GroEL rings and subsequent cis folding of substrate protein.


    MATERIALS AND METHODS
 TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS AND DISCUSSION
 REFERENCES
 
Materials—Single-stranded DNAs of the plasmid pET-EL and pET-ES2 were obtained by infecting Escherichia coli CJ236 cells with helper phage M13KO7 (Amersham Biosciences). Mutant GroES(T19C) was made by Kunkel methods using an oligonucleotide 5'-CAGCAGATTTGCATTCAACTTCTTTACG-3'. GroEL, GroES mutants, and rhodanese were expressed and purified as described (11). GroEL purified by the procedures including gel permeation column chromatography in the presence of 30% methanol (11) contained only a very small amount of contaminated proteins (<0.1 Trp residues/GroEL tetradecamer). MDH from pig heart, hexokinase, ATP, ADP, and AMPPNP were purchased from Roche Diagnostics. Diadenosine pentaphosphate (Ap5A) was purchased from Sigma. Protein concentrations were measured by BCA protein assay (Pierce) and calibrated on the basis of quantitative amino acid analysis.

GroEL Saturated with Unfolded Proteins—Rhodanese (4 µM) or MDH (4 µM) was heat-denatured at 60 °C for 15 min in the buffer (50 mM HEPES-NaOH, pH 7.2, 1 mM EDTA, and 1 mM DTT) containing 1 µM GroEL.2 By this treatment, GroEL was not impaired at all, as shown by its 100% retention of ATPase activity and chaperone activity, whereas substrate proteins were completely inactivated. Temperature was shifted down to 25 °C, and GroEL-unfolded protein complex was isolated with gel permeation HPLC. In the latter stage of this study, we used ultrafiltration (Microcon YM-100, Millipore), which was as effective as HPLC to remove unbound substrate proteins.

cis Folding Assays—To initiate cis folding reactions, the solution containing GroEL loaded with unfolded proteins (rhodanese or MDH) and GroES was mixed with a 3-fold volume of the solution containing the nucleotide (ATP, ADP, or AMPPNP). When indicated, hexokinase was included in the nucleotide (ADP or AMPPNP) solutions. Final concentrations of the components in the reaction mixtures were 1 mM nucleotides, 0.5 µM GroEL saturated with rhodanese or MDH, 1.0 µM GroES, 200 mM glucose, 50 mM HEPES-NaOH, pH 7.2, 200 mM CH3COOK, 10 mM Mg(CH3COO)2, 1 mM DTT and, when indicated, 0.04 units/µl hexokinase. In the case of rhodanese, 20 mM Na2S2O3 was additionally included in the reaction mixtures. For the single turnover ATP hydrolysis experiment, excess ATP was hydrolyzed by adding hexokinase (final concentration, 0.04 units/µl) to the reaction mixture at 3 s after initiation of the reaction. We confirmed that 1 mM ATP in the reaction mixture was quenched completely within next 3 s. To measure the time course of rhodanese folding, aliquots (5 µl) were taken out at indicated times and mixed with 750 µl of the solution containing 100 mM KH2PO4, 150 mM Na2S2O3, and 1 mM EDTA, and recovered rhodanese activities were measured (8, 11). In the experiments to observe the effect of pretreatment with hexokinase, the nucleotide solutions were treated with hexokinase (0.04 units/µl) for 15 min and separated from hexokinase by ultrafiltration (Microcon YM-3). When indicated, 1 mM Ap5A was included in the nucleotide solutions. The abovementioned assays were all carried out at 25 °C. To quantitate the amount of GroES and the substrate protein in the cis cavity, at 2 h of incubation, released GroES and substrate protein were removed by repeated (four times) dilution-ultrafiltration procedures (Microcon YM-100). Subsequently, proteinase K (final concentration, 1 µg/ml) was added to digest non-protective unfolded protein. After a 30-min incubation at 25 °C, phenylmethylsulfonyl fluoride was added at final concentration of 1 mM, and digested products smaller than 100 kDa were removed by ultrafiltration as described above. An aliquot of the resulting solution (~20 µg) was applied to 12% polyacrylamide gel electrophoresis in the presence of SDS, and Coomassie Blue R-250-stained band intensities of substrate proteins and GroES were quantitated by using the public domain NIH Image program (developed at the U.S. National Institutes of Health and available on the Internet at rsb.info.nih.gov/nih-image) and calibrating with band intensities of the known amount of proteins. Additional aliquots of proteinase K-treated, ultrafiltered samples were used for measuring the recovered activities of rhodanese or MDH that corresponded to the cis folding. To release all the proteins from the cis cavity, GroES was detached from GroEL by the addition of an equal volume of 50 mM EDTA to the aliquot. After a 90-min incubation at 25 °C (in which active dimers were formed in the case of MDH), activities of rhodanese and MDH were measured.

Kinetics of GroES Binding to and Release from GroEL—GroES(98C) and GroES(T19C) (0.14 mM) were incubated at 4 °C for 24 h with 2 mM 5(2-iodoacetylaminoethyl) aminonaphthalene-1-sulfonic acid in 25 mM Tris-HCl, pH 7.5, and 10 mM Tris(2-carboxyethyl)phosphine (Molecular Probes). Free labeling reagent was quenched by 10 mM DTT and removed by a Sephadex G-25 column equilibrated with 50 mM HEPES-NaOH, pH 7.2, 200 mM CH3COOK, 10 mM Mg(CH3COO)2, and 1 mM DTT. The fractions containing GroES were concentrated by Ultrafree-4 (Millipore). The molar ratio of the labels to GroES was ~0.2:1 for both GroESs. The labeled GroES(98C) (GroESC) and labeled GroES(T19C) (GroESN) were fully active in GroEL-GroES-dependent folding of rhodanese and in suppression of ATPase activity of GroEL (data not shown). The time course of GroES binding to GroEL was monitored by the fluorescence of GroESN that increased upon binding to GroEL. The solution containing GroESN and nucleotide was mixed at 25 °C with free GroEL or GroEL saturated with unfolded proteins, prepared as described above, using a stopped-flow rapid mixing apparatus (SFM-400, BioLogic) equipped with fluorometer. Final concentrations of GroESN and nucleotide were 0.02 µM and 1 mM, respectively. GroEL concentrations were varied (final concentrations, 0.05, 0.1, 0.15, 0.2, and 0.25 µM). The excitation wavelength was 340 nm, and the emission light above 420 nm was measured. The same experiments were repeated five times, and the data were averaged. The binding was analyzed assuming the simple bimolecular binding scheme, GroEL + GroESN -> GroEL-GroESN. Since the dissociation rate constant (koff) is small enough to be ignored, the association rate constant kon is calculated from linear fitting to the apparent rate constants (kapp) obtained from single exponential fitting at different GroEL concentrations; kapp = kon [GroEL]. The rate of release of GroES from GroEL was estimated from the loss of fluorescence of GroESC from the GroEL-GroESC complexes. The cis ternary GroEL-GroESC-rhodanese complex and the GroEL-GroESC complexes were made by the procedures described above except that 1 µM GroEL and 0.5 µM GroESC were used in the presence or absence of rhodanese. The complexes were incubated at 25 °C with excess amount (final concentration, 10 µM) of non-fluorescent, native GroES to prevent the rebinding of GroESC. Aliquots were taken out at indicated times and applied to a gel permeation HPLC column equilibrated with 50 mM HEPES-NaOH, pH 7.2, 200 mM CH3COOK, 10 mM Mg(CH3COO)2, 0.1 mM ADP, 20 mM glucose, 0.01 units/µl hexokinase, 1 mM DTT, and 0.05% NaN3. The GroESC fluorescence eluted in the GroEL peak was measured with an on-line fluorometer at 490 nm with excitation at 340 nm.


    RESULTS AND DISCUSSION
 TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS AND DISCUSSION
 REFERENCES
 
Elimination of Contaminated ATP by Hexokinase—Trace amount of ATP in ADP and AMPPNP solutions cannot be accurately measured with the usual HPLC analysis, and we applied a luciferase assay. Commercially available ADP and AMPPNP usually are contaminated with ATP, ranging 0.1–2.0%. Purification of ADP using a Dowex ion exchange column decreased the amount of ATP to 0.02–0.05%. Treatment with hexokinase plus glucose was more effective, and ATP contamination in ADP and AMPPNP was decreased down to 0.005–0.009% (10). Taking into account that purified protein could contain trace amounts of adenylate kinase that potentially generate ATP (and AMP) from ADP continuously during the reaction period, we have included hexokinase plus glucose in the reaction mixtures. Hereafter, commercial AMPPNP and ADP that have not been treated with hexokinase are referred to as AMPPNPraw and ADPraw, respectively. Those treated with hexokinase plus glucose are as AMPPNPhex and ADPhex, respectively. Hexokinase was also used for the single turnover ATP hydrolysis experiment (referred to as ATPsingle); hexokinase added at 3 s after initiation of the reaction hydrolyzed all free ATP so that the second chaperonin cycle was prevented. The folding yields of rhodanese and MDH in the cis cavity under the conditions of ATPsingle were 1.1 and 0.60 mol/mol of GroEL-GroES complex, respectively, which were approximately the same as those (1.2 and 0.66 mol/mol) achieved by GroEL(D398A), a mutant deficient in ATP hydrolytic activity. Therefore, it is confirmed that the single turnover reaction of chaperonin can be measured by this procedure.

Saturation of GroEL by Heat-denatured Proteins—At first, we prepared GroEL in which all the binding sites for substrate proteins were saturated with unfolded proteins. Dilution from the concentrated protein solutions containing chemical denaturants such as guanidium hydrochloride or urea into the reaction solution was avoided because of the inhibition effect of the denaturants on GroEL-GroES association (12). Instead, we simply heated the mixtures of GroEL and substrate proteins, either rhodanese or MDH, at 60 °C for 15 min. Rhodanese and MDH were denatured completely by this heat treatment. GroEL is a rather heat-stable protein and remained intact as ensured by the unaffected ATP hydrolysis activity and chaperone activity after the heat treatment (data not shown). Denatured substrate proteins occupied all the substrate protein binding sites of GroEL, and the GroEL loaded with a saturating amount of unfolded proteins (referred as the loaded GroEL) was separated from unbound substrate proteins with gel permeation HPLC or ultrafiltration. The molar stoichiometry of the bound substrate protein to GroEL estimated from band intensities in SDS-PAGE was 2.4 (rhodanese) and 2.5 (MDH). Saturation of substrate protein binding sites of GroEL by this procedure was confirmed as described later.

GroES Binding to the Free and Loaded GroEL—The fluorescently labeled GroESN increases its fluorescence upon binding to GroEL. The extent of increase is ~25%, regardless of nucleotides and the presence or absence of unfolded proteins (Fig. 1, data not shown for ADPhex and AMPPNPhex). Using the fluorescence increase of GroESN as a probe, we measured time courses of binding of GroES to GroEL. GroESN bound rapidly to unloaded GroEL in ATP, ADPhex, and AMPPNPhex with ATP being the fastest and ADPhex the second fastest (Fig. 2A). Binding rates of GroESN in ADPraw and AMPPNPraw were slightly faster than those treated with hexokinase (Table I). The binding reactions for all nucleotides are simulated by single exponential curves, and the calculated association rate constants (kon) are in the same order of magnitude, 107 M–1 s1 (Table I). The kon value of the GroESN binding to unloaded GroEL in ATP (7.5 x 107 M–1 s1) is consistent with the previously reported values 4 x 107 M–1 s1 (13) and 5 x 107 M–1 s–1 (4), ensuring the validity to use GroESN for the binding experiments. These results show that ADP and AMPPNP are as effective as ATP in mediating GroES binding to free GroEL. However, these nucleotides have a very different effect on the GroESN binding to the loaded GroEL (Fig. 2, B–D and Table I). In ATP, GroESN binding to the rhodanese-loaded GroEL and MDH-loaded GroEL were slightly slower than that to free GroEL but still very rapid, completed within 1 s (kon > 107 M–1 s–1) (Fig. 2B). In AMPPNPraw, the rates were slowed down by ~102-fold (Fig. 2C). In AMPPNPhex, the rates were slowed down further; GroES binding to the MDH-loaded GroEL and the rhodanese-loaded GroEL were reached only 40 and 5% after 5 min, respectively. Similarly, the binding of GroESN to the loaded GroEL was slow in ADPraw and even slower in ADPhex (Fig. 2D). Although these slow GroES binding to the loaded GroEL in ADP and AMPPNP have not been known previously, this can be expected because GroES and unfolded protein compete for the overlapping binding sites on the GroEL. Rather, a new mechanism is required to understand this rapid GroES binding in ATP to the loaded GroEL.



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FIG. 1.
Fluorescence change of GroESN induced by binding to GroEL. Fluorescence spectra of GroESN with excitation wavelength at 340 nm are shown. none, in the absence of GroEL; none/ATPsingle, GroEL-GroESN formed in ATPsingle; rho/ATPsingle, rhodanese-loaded GroEL-GroES formed in ATPsingle. Spectra of GroEL-GroESN formed in ADPhex and in AMPPNPhex were also measured but not shown because they overlapped with those of none/ATPsingle completely. Experimental details are described under "Materials and Methods." Em, emission; A.U., arbitrary units.

 


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FIG. 2.
Binding of GroES to free GroEL and the loaded GroEL in various nucleotides. Binding was monitored with the fluorescence change of GroESN. The reactions were initiated by mixing the solution containing GroESN and nucleotide with the solution containing free GroEL or the loaded GroEL. Final concentrations of GroEL, GroESN, and nucleotides were 0.1 µM, 0.02 µM, and 1 mM, respectively. Single exponential fitting curves that were used to obtain association rate constants in Table I are shown in white lines. A, GroESN binding to free GroEL in ATP, AMPPNPhex, and ADPhex. A.U., arbitrary units. B, GroESN binding to the rhodanese-loaded GroEL and the MDH-loaded GroEL in ATP. C, GroESN binding to the rhodanese-loaded GroEL and the MDH-loaded GroEL in AMPPNPraw and AMPPNPhex. D, GroESN binding to the rhodanese-loaded GroEL and the MDH-loaded GroEL in ADPraw and ADPhex.

 

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TABLE I
Kinetic constants of GroES binding and release

The apparent association rate constants were obtained by fitting the curves of GroESN binding to GroEL as typically shown in Fig. 2. The values of kon were calculated from linear fitting to the apparent rate constants at several GroEL concentrations (see "Materials and Methods"). Dissociation rate constants were obtained from the data in Fig. 4.

 



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FIG. 4.
Release of GroES from the GroEL-GroES complex. Release of GroES was assessed by the loss of fluorescent GroESC from the GroEL-GroESC complexes, which had been formed in ATPsingle, AMPPNPhex, or ADPhex. Twenty-fold molar excess of native GroES was added at time 0. Aliquots were loaded to gel permeation HPLC at the indicated times, and the remaining GroESC in the GroEL-GroES fraction was measured. Release of GroESC from the GroEL-GroESC-rhodanese cis ternary complex formed in ATPsingle is also shown. rho/ATPsingle, rhodanese-loaded GroEL-GroES formed in ATPsingle.

 
When the substrate binding sites of GroEL are not saturated with unfolded proteins, it is predicted that rapid GroES binding in ADPhex to free GroEL rings in partially loaded GroEL can be observed. Indeed, in ADPhex, GroESN bound rapidly to two-thirds of the 1:1 (molar ratio of rhodanese and GroEL) rhodanese-loaded GroEL and to a quarter of the 2:1 rhodanese-loaded GroEL (Fig. 3). The rapid binding was no longer observed for the 2.5:1 rhodanese-loaded GroEL, indicating that all the substrate binding sites of GroEL were occupied by unfolded rhodanese. These results showed that 2.5 rhodanese and MDH can bind to GroEL even if GroEL has only two rings for substrate protein binding. This discrepancy may be due to the error in estimation of protein concentration or the occasional binding of two substrate proteins to one GroEL ring as reported using citrate synthase as substrate protein (14).



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FIG. 3.
GroES binding to the partially loaded GroEL. Binding was monitored with the fluorescence change of GroESN. Binding reactions were initiated by mixing the solution containing GroESN and ADPhex with the solution containing the rhodanese-GroEL complexes with rhodanese:GroEL molar ratios 1:1, 2:1, and 2.5:1. Final concentrations of GroEL, GroESN, and ADPhex were 0.05 µM, 0.05 µM, and 1 mM, respectively. A.U., arbitrary units.

 

Dissociation of GroES from GroEL—Dissociation of GroES from GroEL was measured by the exchange of fluorescently labeled GroESC bound on GroEL with an excess amount of unlabeled GroES. As shown in Fig. 4, the GroEL-GroESC complexes formed in ATPsingle and ADPhex without substrate protein are surprisingly stable; only 10% of the GroEL-GroESC complexes released GroESC after 1 week. The GroEL-GroESC complex formed in ATPsingle becomes identical to that in ADPhex since the bound ATP in the complex is hydrolyzed to ADP. This result shows the extraordinary stability of the GroEL-GroESC complexes in ADP. The complex without substrate protein formed in AMPPNPhex was relatively unstable and decayed in 2 days. The GroEL-GroESC-rhodanese complex formed in ATPsingle was slightly less stable than the GroEL-GroESC complex in ATPsingle in the absence of unfolded protein. This instability in the presence of rhodanese may be due to the stimulation of GroES release by substrate protein binding to trans ring (4). Calculated dissociation rate constants (koff) (Table I) are smaller than the previously reported value (3.8 x 105 s) estimated from the dissociation of ADP moiety from the GroEL-GroES complex (15). The reason of this discrepancy is not known, but there is a possibility that a trace amount of contaminated ATP would stimulate the exchange of bound ADP. The estimated dissociation constants of GroES binding to GroEL are extremely small (~10 fM) and comparable with the dissociation constant of the biotin-avidin binding. This strong GroES binding may enable GroES to bind the loaded GroEL, the GroES binding site of which is covered by substrate protein.

cis Folding of Rhodanese—From the slow binding of GroES to the rhodanese-loaded GroEL in ADPhex and AMPPNPhex, it can be predicted that formation of the cis ternary complex and the cis folding cannot occur in these nucleotides. To examine this, we compared the effect of these nucleotides on the chaperonin-assisted folding of rhodanese. Since it has been known that rhodanese folds in the cis cavity but does not fold spontaneously (16), all the recovered rhodanese activity can be attributed to the result of the cis folding. The reaction was started by mixing the rhodanese-loaded GroEL, GroES, and nucleotides (Fig. 5A). In ATP, the yield of recovered rhodanese reached to more than 80% of the total bound rhodanese in 60 min. In ATPsingle, the recovered rhodanese was nearly 50%, showing that unfolded rhodanese bound in one GroEL ring is folded in the cis cavity after single ATP hydrolytic cycle was terminated. We observed significant recovery of rhodanese activity also in ADPraw (~50%) and AMPPNPraw (~20%). The same results were reported previously by others (79), and it has been thought that ADP and AMPPNP can substitute ATP to some extent for the chaperonin function. However, hexokinase treatment diminished rhodanese activity in ADPhex and AMPPNPhex (Fig. 5A). To determine which is the cause of ATP contamination, contaminated ATP or ATP production by contaminated adenylate kinase, rhodanese activity was measured using pre-hexokinase-treated ADP and AMPPNP (ADPpre-hex and AMPPNPpre-hex) that were treated with hexokinase and separated from hexokinase by ultrafiltration (Fig. 5B). Approximately 50% of rhodanese was recovered in ADPpre-hex. The initial lag period in the rhodanese recovery in ADPpre-hex can be interpreted as the time required for the accumulation of ATP by adenylate kinase. Indeed, in the presence of Ap5A, a potent inhibitor of adenylate kinase (17), rhodanese reactivation disappeared in ADPraw, whereas it was not affected in ATP. The reason for no rhodanese recovery in AMPPNPpre-hex may be explained by the low concentration of contaminated ADP that is not enough for adenyate kinase to produce ATP. These results lead to conclusion that the causes for rhodanese recovery in ADPraw and AMPPNPraw are trace amounts of contaminated adenylate kinase and contaminated ATP, respectively.



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FIG. 5.
Recovery of rhodanese activities assisted by GroEL and GroES in various nucleotides. Recovery yields were expressed as mol of recovered enzyme per mol of GroEL. A, recovery in ATP, ATPsingle, AMPPNPraw, ADPraw, AMPPNPhex, and ADPhex. B, recovery in AMPPNPpre-hex and ADPpre-hex that had been pretreated with hexokinase and effect of 1 mM Ap5A, an inhibitor of adenylate kinase. Ap5A was also added to the reaction mixture for ATP as a control. Note that hexokinase was included in the reaction mixtures for ATPsingle (after 3 s), AMPPNPhex, and ADPhex during the reaction period (A) but was removed from the reaction mixtures of AMPPNPpre-hex and ADPpre-hex prior to the initiation of the reaction (B). No rhodanese recovery was observed in the absence of nucleotide (data not shown).

 

Formation of the cis Ternary Complex—We assessed the formation of the cis ternary complexes by using protease treatment; the substrate proteins entrapped in the cis cavity are protected from the protease digestion, whereas those bound to the trans ring are readily digested as well as free unfolded proteins (2, 16). The samples incubated for 120 min as in Fig. 5A were ultrafiltrated to remove free GroES, digested by proteinase K, and ultrafiltrated to remove proteinase K and digested polypeptide. Three kinds of GroEL complexes, GroEL, GroEL-GroES, and the GroEL-GroES-rhodanese cis ternary complex, should exist after this procedure. The relative populations of GroEL, GroES, and rhodanese were estimated from the band intensities in the CBB-stained SDS-PAGE (Fig. 6A). The rhodanese-loaded GroEL contained 2.4 mol rhodanese/mol of GroEL (lane 1), and all rhodanese molecules were digested by proteinase K (lane 2). In ATP, the folded rhodanese molecules had been removed by ultrafiltration, and GroEL-GroES complexes remained (lane 3). In AMPPNPraw, ADPraw, and ATPsingle, significant amount of proteinase K-resistant rhodanese were observed (lanes 4–6). Molar ratios among GroEL, rhodanese, and GroES in these three lanes were 1.0:0.8–1.4: 0.7–1.1, indicating that the GroEL-GroES-rhodanese cis ternary complex was formed in these nucleotides. The rhodanese molecules held in the cis cavity of these complexes had finished folding because rhodanese activity of these complexes is equivalent to the estimated amount of rhodanese from SDS-PAGE (Fig. 6A, lower panel). In contrast, in AMPPNPhex and ADPhex, no rhodanese and a faint amount of GroES were observed (lanes 7 and 8), suggesting that GroES cannot bind to the rhodanese-loaded GroEL or can form only unstable GroEL-GroES-rhodanese ternary complex. Slow GroES binding to rhodanese-loaded GroEL in AMPPNPhex and ADPhex (Fig. 2, C and D) can be due to the formation of such unstable complex. Next, we used MDH as a substrate protein (Fig. 6B). Molar ratios among GroEL, MDH, and GroES in AMPPNPraw, ADPraw, and ATPsingle (lanes 4–6) were 1.0:0.8–1.1:1.0–1.1, indicating the stoichiometric formation of the cis ternary complexes as well as rhodanese. The results of AMPPNPhex (lane 7) and ADPhex (lane 8) are different from those of rhodanese. In AMPPNPhex, smaller amounts of MDH than ATPsingle were encapsulated in the cis cavity, whereas rhodanese was not encapsulated. In ADPhex, no MDH band was observed as rhodanese; however, GroES was bound to GroEL in contrast to rhodanese. Probably, MDH was gradually released from GroEL, and then GroES bound to these newly available binding sites. Folding of MDH in the cis cavity was assessed by measuring MDH activity after the MDH dimer was formed by releasing the MDH monomer from the cis cavity using EDTA to open the GroES cap (Fig. 6B, lower panel). Recovered MDH activity of each sample roughly corresponds to the amount of MDH in SDS-PAGE. ADPhex did not support cis folding of MDH, but AMPPNPhex did so in some extent. This ATP analogue seems to mimic the action of ATP in this case by a yet unknown mechanism.3



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FIG. 6.
Formation of the cis ternary complex in various nucleotides. Rhodanese (A) and MDH (B) were used as substrate proteins. Lane 1, substrate protein-loaded GroEL without proteinase K (ProK) treatment. Lane 2, substrate protein-loaded GroEL treated with proteinase K. Lanes 3–8, substrate protein-loaded GroEL and GroES were subjected to the following procedures: a 2 h-incubation in the indicated nucleotides, ultrafiltration (100-kDa cut), proteinase K treatment, ultrafiltration (100-kDa cut), and SDS-PAGE analysis. Nucleotides included during a 2 h-incubation were: lane 3, ATP; lane 4, AMPPNPraw; lane 5, ADPraw; lane 6, ATPsingle; lane 7, AMPPNPhex; lane 8, ADPhex. Another aliquot of the solution after the second ultrafiltration was treated with EDTA to detach GroES, and the activities of rhodanese and MDH released from the cis cavity were measured to estimate the cis folding yields that were expressed as mol of recovered enzyme per mol of GroEL. (lower panels). Experimental details are described under "Materials and Methods."

 

Conclusions—The previously reported ability of ADP, less efficient than that of ATP in the formation of the cis ternary complex, has made the current conception of the ATP-dependent chaperonin function ambiguous. However, our results clearly show that the cis ternary complex is not formed and that the cis folding does not occur in ADP. Also, cis folding of substrate proteins in ADP has been caused by the contaminated adenylate kinase because its inhibitor, diadenosine pentaphosphate, suppressed the cis folding of rhodanese. It should be noted that the direct measurement of adenylate kinase activity contaminated in the purified GroEL is hard to detect due to the immediate hydrolysis of produced ATP by GroEL. In contrast, AMPPNP appears to be able to mimic the action of ATP to some extent; it supports cis folding for MDH but not for rhodanese. This work sheds light on the key question on the chaperonin function; how can ATP enable rapid binding of GroES to the substrate protein-loaded GroEL whose binding sites for GroES are occupied by the substrate protein?


    FOOTNOTES
 
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{ddagger} To whom correspondence should be addressed. Fax: 81-45-924-5277; E-mail: myoshida{at}res.titech.ac.jp.

1 The abbreviations used are: AMPPNP, 5'-adenylyl-{beta},{gamma}-imidodiphosphate; AMPPNPhex, AMPPNP that is always exposed to hexokinase and glucose; AMPPNPraw and ADPraw, commercial AMPPNP and ADP that have not been exposed to hexokinase; ADPhex, ADP that is always exposed to hexokinase and glucose; ATPsingle, ATP exposed to hexokinase at 3 s after initiation of the reaction, by which time only a single turnover of ATP hydrolysis of GroEL can occur; MDH, malate dehydrogenase; GroESN, GroES(T19C) labeled by 5(2-iodoacetylaminoethyl) aminonaphthalene-1-sulfonic acid; GroESC, GroES(98C) labeled by 5(2-iodoacetylaminoethyl) aminonaphthalene-1-sulfonic acid; Ap5A, diadenosine pentaphosphate; DTT, dithiothreitol; HPLC, high pressure liquid chromatography. Back

2 Concentrations of GroES and GroEL are all expressed as heptamer and tetradecamer, respectively, in this article. Back

3 In AMPPNPhex and ADPhex, MDH in the MDH-loaded GroEL appeared to be gradually released from GroEL, and then GroES bound tightly to the newly available binding sites, because recovery of some MDH activity was observed even before the addition of EDTA (data not shown). In this case, recovery occurred much more slowly in AMPPNPhex than in ADPhex, indicating higher affinity of MDH to GroEL in AMPPNPhex than in ADPhex, which is consistent with slower GroESN binding to MDH-loaded GroEL in AMPPNPhex than in ADPhex (Fig. 2, C and D). The small cis folding of MDH in AMPPNPhex detected in Fig. 6B may be related to this relatively strong affinity of MDH to GroEL in AMPPNPhex and accidental entrapping of MDH in the cis cavity, whereas GroES competes for its binding sites. Back


    ACKNOWLEDGMENTS
 
We are grateful to Drs. T. Hisabori, E. Muneyuki, and H. Taguchi for valuable discussion, Drs. K. Kinosita, T. Kawashima, and T. Masaike for the use of the stopped-flow apparatus, and S. Murayama, T. Inoue, and R. Suno for experimental assistance.



    REFERENCES
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 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS AND DISCUSSION
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