From the Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Aoba-ku, Sendai 980-8579, Japan and the § National Institute for Advanced Industrial Science and Technology (AIST), Hokkaido Center, Tsukisamu-Higashi, Toyohira-ku, Sapporo 011-8511, Japan
Received for publication, October 4, 2002, and in revised form, November 18, 2002
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ABSTRACT |
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To study the role of hydrogen bonding via
interfacial water molecules in protein-protein interactions, we
examined the interaction between hen egg white lysozyme (HEL) and its
HyHEL-10 variable domain fragment (Fv) antibody. We constructed three
antibody mutants (L-Y50F, L-S91A,
and L-S93A) and investigated the interactions between the
mutant Fvs and HEL. Isothermal titration calorimetry indicated that the
mutations significantly decreased the negative enthalpy change (8-25
kJ mol Specific recognition of ligands by proteins is fundamentally
important in biological phenomena. Recent high resolution x-ray crystallographic determinations of the structures of various
protein-protein complexes have shown that protein-protein interfaces
are generally characterized by a high degree of both shape and charge
complementarity (1-3). The specificity and affinity of protein-protein
interactions are in principle created by the complementarity of the
interface surfaces, which allows the formation of various noncovalent
bonds (hydrogen bonds, salt bridges, and van der Waals interactions) (1-3). Solvent water molecules, however, have been observed at protein-protein interfaces, mediating imperfect surface complementarity via hydrogen bond formation (1, 4-7).
Solvent plays a significant role in biologically important protein
association systems (8), e.g. the adhesion interface of the
cell adhesion protein cadherin (9), the barnase-barstar complex (5),
the cytochrome c-cytochrome c oxidase interaction (10, 11), antigen-antibody interactions (12-16), the bacterial periplasmic protein (OppA)-peptide interaction (17, 18), the DNA-repressor interaction (19, 20), the interaction between T-cell
receptor and self-peptide major histocompatibility complex antigen
(21), and the natural killer cell receptor-major histocompatibility complex antigen interaction (22). Ladbury (5) proposed that water at
the interface of a complex can participate in various types of
interaction, yet can also lead to increased specificity and affinity.
However, the energetic contribution of hydrogen-bonded interfacial
water molecules to protein-protein interactions has been determined for
only a few complexes (18, 23).
Elucidation of the roles of the hydrogen bonds involved in
antigen-antibody complementary association requires both structural and
thermodynamic information (24). X-ray crystal analysis can clarify the
structural aspects of the complementarity of the interactions (25-28),
and titration calorimetry can provide useful information for the
quantitative assessment of the energetic contribution of residues to
the interaction (29-31). Thus, the combination of these two approaches
should be especially valuable in providing insight into hydrogen
bonding via interfacial water molecules.
We have focused on the interaction between hen egg white lysozyme
(HEL)1 and its monoclonal
antibody HyHEL-10 (32-34), whose structural features have been
analyzed by x-ray crystallography in the Fab-HEL (35) and Fv-HEL (36)
complexes. The bacterial expression system of the HyHEL-10 Fv fragment,
which consists of the associated variable domains of an antibody, has
been established (37-39); and the Fv-HEL interactions have been
investigated by using mutant Fv fragments (40-45). The combination of
thermodynamic data with structural results should be a powerful tool
for the precise description of the mutant Fv-HEL interactions.
In the wild-type Fv-HEL complex, Kondo et al. (36) observed
12 water molecules bridging the imperfect antigen-antibody interface (Fig. 1). Kondo's x-ray crystallographic
study indicated that the temperature factor (B factor) of
these water molecules is small, and thus, the contribution of these
solvent water molecules to the Fv-HEL interaction is assumed to be
significant. Therefore, thermodynamic analyses of mutant Fv-HEL
interactions combined with structural analyses of the Fv-HEL complexes
should give further insight into the molecular basis for the role of
hydrogen bond formation via interfacial water molecules in the
interaction. In this report, we have focused on three residues
(Tyr50, Ser91, and Ser93) in the
light chain. These residues participate in the formation of the
hydrogen bond network (Fig. 1B), and thus, the mutational analyses should give further insight into the role of the hydrogen bonds.
1), despite some offset by a favorable entropy
change. X-ray crystallography demonstrated that the complexes had
nearly identical structures, including the positions of the interfacial
water molecules. Taken together, the isothermal titration calorimetric
and x-ray crystallographic results indicate that hydrogen bonding via
interfacial water enthalpically contributes to the Fv-HEL interaction
despite the partial offset because of entropy loss, suggesting that
hydrogen bonding stiffens the antigen-antibody complex.
INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
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Fig. 1.
Hydrogen bonding via interfacial water
molecules at the HyHEL-10 Fv-HEL interface. C stick
diagram of the wild-type Fv-HEL complex. VL, green; VH,
sky blue; HEL, pink. The residues investigated in
this report are drawn in blue. A, overall
structure. B, local structures around the target sites.
Generated with WebLab Viewer (Molecular Simulations Inc., San Diego,
CA). The antibody residues are numbered according to Kabat et
al. (75).
Here, using isothermal titration calorimetry and x-ray crystallography,
we report the effect on complex formation of truncating atoms of
antibody side chains participating in hydrogen bond formation via water
molecules. On the basis of our thermodynamic and structural results, we
discuss the role of the hydrogen bonds in the protein antigen-antibody interaction.
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EXPERIMENTAL PROCEDURES |
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Materials--
All enzymes for genetic engineering were obtained
from Takara Shuzo (Kyoto, Japan), Toyobo (Osaka, Japan), and New
England Biolabs (Beverly, MA).
Isopropyl--D-thiogalactopyranoside was obtained from
Wako Fine Chemicals Inc. (Osaka, Japan). All other reagents were of
biochemical research grade. The antigen, HEL (Seikagaku-Kogyo, Tokyo,
Japan), was purified by ion exchange chromatography on SP-Sepharose FF
(Amersham Biosciences, Tokyo, Japan), followed by gel filtration on
Superdex 75 pg equilibrated with phosphate-buffered saline. The
eluate was lyophilized and dissolved in water at a concentration of
0.54 mM prior to use.
Site-directed Mutagenesis-- Site-directed mutagenesis was performed with phagemid pTZ18U (Bio-Rad) according to the method of Kunkel et al. (46). The DNA oligonucleotide primers for mutation at sites 50, 91, and 93 of VL were 5'-CTGATCAAGTTCGCGAGCCAG-3', 5'-TGCCAGCAGGCGAACAGCTGG-3', and 5'-CAGTCGAACGCCTGGCCGTAC-3', respectively (mutated sites are underlined). The correctness of the mutations was confirmed by DNA sequencing (ABI 310 Genetic Analyzer, Applied Biosystems, Tokyo, Japan).
Preparation of HyHEL-10 Mutant Fv-- The mutant Fv fragment was prepared essentially as described previously (35). The purified Fv fragment was concentrated by ultrafiltration centrifugation (0.45-µm membrane) at 3000 × g (Centriprep-10, Japan-Millipore, Tokyo). The purities of the proteins were confirmed by SDS-PAGE in the buffer system described by Laemmli (47).
Inhibition Assay of HEL Enzymatic Activity-- The experimental procedure for the inhibition assay was essentially that of Ueda et al. (37). In brief, the various concentrations of the Fv fragment were mixed with 1.5 µM HEL and incubated at 25 °C for 1 h in 30 µl of phosphate-buffered saline. Each mixture was then added to 970 µl of 50 mM NaH2PO4 buffer (pH 6.20, adjusted with NaOH) containing 340 µg of Micrococcus luteus cells. The initial rate of the decrease in A540 nm was monitored at 25 °C.
Isothermal Titration Calorimetry-- The thermodynamic parameters of the interactions between HEL and the wild-type or mutant Fv fragments were determined by isothermal microtitration calorimetry (VP-ITC, MicroCal, Northampton, MA) (48) under conventional conditions as follows. In a calorimeter cell, a 5 µM solution of HEL in 50 mM phosphate buffer (pH 7.2) containing 200 mM NaCl was titrated with a 50 µM solution of Fv in the same buffer at five temperatures (20, 25, 30, 35, and 40 °C). The ligand solution was injected 25 times in portions of 10 µl over 20 s. Thermogram data were analyzed with the computer program Origin version 5 (MicroCal) after subtraction of the heats of dilution of Fv fragment into buffer and buffer into HEL (obtained in separate experiments).
To correlate thermodynamic data with structural data, the thermodynamic parameters of the interactions were determined under crystallization conditions as follows. In a calorimeter cell, a 5 µM solution of HEL in 100 mM Hepes buffer (pH 7.6), 1% (w/v) polyethylene glycol 6000, 15% glycerol, and 1% (w/v) 2-methyl-2,4-pentanediol was titrated with a 50 µM solution of Fv in the same buffer at four temperatures (20, 25, 30, and 35 °C). The ligand solution was injected 25 times in portions of 10 µl over 20 s. Thermogram data were analyzed as described above after subtraction of the heats of dilution of the Fv fragment into buffer and buffer into HEL (obtained in separate experiments).
The enthalpy change (H) and binding constant
(Ka) for the antigen-antibody interaction were
directly obtained from the experimental titration curve. The Gibbs free
energy change (
G =
RTlnKa) and the entropy change
(
S = (
G +
H)/T) for the association were calculated from
H and Ka. The heat capacity change
(
Cp) was estimated from the temperature dependence of the enthalpy change.
Estimation of Protein Concentration--
The concentration of
HEL was estimated by using A
Crystallization, Data Collection, and Structural Determination of the HyHEL-10 Mutant Fv-HEL Complexes-- The HyHEL-10 mutant Fv fragment-HEL complexes were crystallized as described previously (36). The best crystals of the Fv-HEL complexes were grown from 100 mM Hepes buffer (pH 7.6-7.8), 9-11% (w/v) polyethylene glycol 6000, 15% glycerol, and 7-9% (w/v) 2-methyl-2,4-pentanediol. They were elongated bipyramid-shaped crystals.
All crystallographic data were collected at 100 K using synchrotron radiation on beam line 6A of the Photon Factory (Tsukuba, Japan) and a Weissenberg camera (50). The diffraction images were integrated with the hkl program DENZO, and the intensity data were processed with SCALA and AGROVATA in the CCP4 suite (51). The crystallographic data and the statistics of the data collection are summarized in Table I.
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The crystals of the three mutant Fv-HEL complexes were isomorphous with
the crystal of the wild-type complex. The structures of the Fv-HEL
complexes were determined by a molecular replacement method with the
program AMoRe (52) in the CCP4 package. The model coordinates of the
mutant complexes were derived from the structure of the wild-type
complex (Protein Data Bank ID code 1c08). Refinements of the structures
of the Fv-HEL complexes were carried out with the programs XPLOR (53)
and REFMAC (54) as described previously (36). The atomic parameters of
the mutant Fv-HEL complexes have been deposited in the Protein Data
Bank (ID codes 1J1O, 1J1P, and 1J1X for L-Y50F,
L-S91A, and L-S93A, respectively).
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RESULTS |
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Secretory Expression, Preparation, and Spectral Properties of Mutant Fv Fragments
To determine the energetic contribution of hydrogen bonds bridging the imperfect interface of the HyHEL-10 Fv-HEL complex to the antigen-antibody interaction, three mutants (L-Y50F, L-S91A, and L-S93A) were constructed and expressed in Escherichia coli. The mutant Fv fragments were purified by affinity chromatography using HEL-Sepharose, followed by gel filtration on Superdex 75 pg. Purities of greater than 95% were obtained by this procedure. The final yield of each mutant was greater than 10 mg/liter of culture.
The peptide and aromatic circular dichroism (CD) spectra of the mutants under the native condition (pH 7.2, 25 °C) were measured to examine the effect of mutation on the conformational stability. Comparison of these CD spectra with those of the wild-type (WT) revealed no differences in the far- and near-UV CD spectra of the wild-type Fv and mutant Fv fragments (data not shown), indicating that the secondary and tertiary structures of the mutants are quite similar to the structure of WT. Thus, the mutations have no significant influence on the overall structure.
Inhibition of Enzymatic Activity of Hen Lysozyme by Mutants
Tsumoto, Kumagai, and co-workers (37, 38, 41) have shown that the
HyHEL-10 Fv fragment inhibits the enzymatic activity of its antigen,
HEL, in the presence of a slight molar excess of the Fv fragment. Thus,
we investigated the inhibition of the enzymatic activity of HEL by
three mutants (L-Y50F, L-S91A, and L-S93A) (Fig. 2).
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The L-S93A mutant Fv, L-S91A mutant Fv, and wild-type Fv fragments showed identical inhibition activities, whereas the L-Y50F mutant Fv fragment showed a slight decrease in inhibition activity toward HEL, suggesting that the L-Y50F, L-S91A, and L-S93A mutations do not lead to a significant reduction of the affinity for the target.
Thermodynamic Analysis of the Interactions between Hen Lysozyme and Mutant Fv Fragments
To investigate the interactions between the mutants and HEL from a
thermodynamic viewpoint, we carried out an isothermal titration calorimetry (ITC) study of the association between the mutant Fv
fragments and lysozyme (41-45) (Fig. 3).
We initially performed ITC at five temperatures under conventional
conditions, i.e. phosphate buffer. Thermodynamic parameters
(30 °C, pH 7.2) calculated from the titration curves are presented
in Table II, and the temperature dependence of the enthalpy changes are shown in Fig.
4A.
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The binding enthalpies for each of the mutant Fv-HEL interactions
relative to the wild-type Fv-HEL interaction were decreased by 24.9, 11.0, and 8.5 kJ mol1 at 30 °C for L-Y50F,
L-S91A, and L-S93A, respectively (Table II).
The binding constants for the mutant interactions were smaller than the
binding constant for the wild-type interaction, resulting in a smaller
change in the Gibbs energy. These results indicate that the decreases
in the negative enthalpy change for each mutant Fv fragment were
compensated for by the decrease in the negative entropy change, leading
to the slight decrease in affinity. From the temperature dependence of
the enthalpy change shown in Fig. 4A, the heat capacity
change was estimated to be
1.62 to
1.86 kJ mol
1
K
1 at 30 °C for the mutant Fvs.
To correlate the results obtained from ITC to the crystallographic study, we next performed an ITC study of the association at four temperatures under the crystallization buffer conditions. Thermodynamic parameters (30 °C, pH 7.6) calculated from the titration curves are presented in Table III, and the temperature dependence of the enthalpy changes are shown in Fig. 4B.
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The binding enthalpies for each of the mutant Fv-HEL interactions
relative to the wild-type Fv-HEL interaction were decreased by 27.5, 18.4, and 5.1 kJ mol1 at 30 °C for L-Y50F,
L-S91A, and L-S93A, respectively (Table III).
The values of the binding constants (Ka) for the L-Y50F and L-S91A mutant interactions were
smaller than the Ka for the wild-type interaction,
whereas the Ka for the L-S93A mutant
interaction was almost identical to the Ka for the
wild-type interaction. These results indicate that the decreases in the
negative enthalpy change for each mutant Fv fragment were compensated
for by the decrease in the negative entropy change, leading to the
slight decrease in, or almost identical, affinity. From the temperature
dependence of the enthalpy change shown in Fig. 4, the heat capacity
change was estimated to be
1.28 to
2.05 kJ mol
1
K
1 at 30 °C for the mutant Fvs.
Crystal Structures of Mutant Fv-HEL Complexes
To precisely describe the mutant Fv-HEL interactions from the
structural viewpoint, we determined the crystal structures of the three
mutant-HEL complexes (Fig. 5).
Crystallographic data are summarized in Table I. The maximum resolution
of the x-ray data used in the refinements was 1.8 Å, and the
R factors of the refined structures were 0.189-0.194. The
free R factors were within 3% of the R
factors.
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The mutant Fv-HEL complexes were superimposed onto the wild-type Fv-HEL
complex. The root mean square differences between the C atoms of the
mutant Fv structures and those of the wild-type Fv structure are
summarized in Table IV. The results
demonstrated that the overall structures of the HyHEL-10 mutant Fv-HEL
complexes are similar to the structure of the HyHEL-10 wild-type Fv-HEL complex.
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No major changes in the relative orientations of VL, VH, and HEL were observed in the mutant Fv-HEL complexes (data not shown). The calculated interfacial areas of the mutant Fv-HEL complexes were 1853, 1879, and 1851 Å2 for the L-Y50F, L-S91A, and L-S93A mutant complexes, respectively (data not shown). Among 330 water molecules observed in the wild-type complex, 296, 307, and 296 water molecules in L-Y50F, L-S91A, and L-S93A complexes, respectively, have been completely on the same position as wild-type, and almost all of other water molecules exist near the position in the wild-type. These results also suggest that the overall structures, including the interfacial areas (water molecules), of the mutant Fv-HEL complexes are almost the same as the overall structure of the wild-type Fv complex.
Local Structural Changes Observed in Mutant Fv-HEL Complexes
L-Y50F Mutant-HEL Complex--
Although significant
structural changes occurred around sites 17-19 of the antigen HEL in
the L-Y50F Fv-HEL complex, no structural differences were
observed around other sites, including the mutated site (Fig.
6). In addition, the locations of the
interfacial water molecules were unchanged. Thus, two hydrogen bonds
involving the hydroxyl group of Tyr50 in CDR-L2 were
removed because of the mutation, and no structural changes involving
interfacial water molecules and the same number of hydrogen bonds as
wild-type were observed.
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L-S91A Mutant-HEL Complex--
No structural changes
were observed in the Fv fragment of the L-S91A Fv-HEL
complex, except at the mutated site. One water molecule was newly
introduced into the mutation site (W3; the | 2F0 F1 |
electron density map of the WT-HEL complex around L-S91A is
shown in Fig. 7A, and the
corresponding region of the L-S91A-HEL complex is shown in
Fig. 7B), bridging L-Asn32,
L-His34, and a water molecule (W13,
corresponding to W2 in the wild-type complex), and this new water
molecule contributed to the formation of the hydrogen-bonding network
at the interface. The B factor of the newly introduced water
molecule (W3) is estimated to be 12.5, which is smaller than the
average value (16.8) of the C
chains of the complex. Thus, two
hydrogen bonds have been increased, and no other structural changes
have been induced because of the mutation.
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L-S93A Mutant-HEL Complex--
In contrast to the
structures of the L-Y50F Fv-HEL and L-S91A
Fv-HEL mutant complexes, the structures of the antigen and antibody of
the L-S93A mutant Fv-HEL complex were unchanged except for deletion of one hydrogen bond because of the mutation (Fig.
8). Thus, one hydrogen bond has been
removed because of the mutation, and no other structural changes have
been induced.
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DISCUSSION |
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Many researchers have proposed that interfacial water molecules make important contributions to the specificity and affinity of antigen-antibody interactions (6-16). To investigate the energetic contribution of hydrogen bonding via interfacial water molecules, we constructed three mutants (L-Y50F, L-S91A, and L-S93A) and investigated the interactions of these mutants with HEL by thermodynamic and structural analyses. As reported in previous work (8, 55) and in this study, the mutations do not lead to major structural changes in the Fv fragment, and the stabilities of the mutant Fv fragments are almost identical to the stability of the wild-type Fv fragment. Thus, the structural and thermodynamic changes in the antigen-antibody mutant complexes do not originate from changes in the structure of the mutants in the antigen-free state. In the following sections, we discuss and correlate our thermodynamic and structural findings.
Thermodynamic Analysis of Mutant Fv-HEL Interactions--
The
values for both the enthalpic (H) and the entropic
(
T
S) contributions to the interaction
between the mutants and HEL became increasingly positive in the order
L-S93A < L-S91A < L-Y50F. The values of the binding constants
(Ka) for the L-S91A mutant-HEL and
L-S93A mutant-HEL interactions were slightly smaller than
the value of Ka for the wild type-HEL interaction, whereas Ka for the L-Y50F mutant-HEL
interaction was significantly smaller than Ka for
the wild type-HEL interaction, resulting in a smaller change in the
Gibbs energy (
G). These results indicate that deletion
of hydrogen bonds via interfacial water molecules in the HyHEL-10
Fv-HEL complex is enthalpically unfavorable and entropically favorable,
suggesting that the hydrogen bonds mediated by interfacial water
molecules in the HyHEL-10 Fv-HEL interaction make an enthalpic
contribution to the interaction. Substitution of Tyr53 with
Phe also led to decreases in both negative enthalpy and entropy changes
(42). Thus, we conclude that hydrogen bonding via interfacial water
molecules in the HyHEL-10-HEL complex makes an enthalpic contribution
to the interaction.
To correlate the results obtained from the thermodynamic analyses with
structural information, we next performed ITC experiments under
crystallization conditions (100 mM Hepes buffer (pH 7.6), 15% glycerol, 1% (w/v) polyethylene glycol 6000, and 1% (w/v) 2-methyl-2,4-pentanediol). The values for both the enthalpic
(H) and entropic (
T
S)
contributions to the interaction between the mutants and HEL became
increasingly positive in the same order as in the case of phosphate
buffer. The values of the binding constants (Ka) for
the L-S91A mutant-HEL and L-S93A mutant-HEL interactions were slightly smaller than the value of
Ka for the wild type-HEL interaction, whereas
Ka for the L-Y50F mutant-HEL interaction
was significantly smaller than Ka for the wild
type-HEL interaction, resulting in a smaller change in the Gibbs energy
(
G). These results correlate qualitatively well with
the data obtained with the phosphate buffer; deletion of hydrogen bonds
via interfacial water molecules in the HyHEL-10 Fv-HEL complex is
enthalpically unfavorable and entropically favorable.
The affinity of the antibody, including mutants, for the target was significantly reduced in the crystallization buffer, which included polyethylene glycol 6000, glycerol, and 2-methyl-2,4-pentanediol (cf. Tables II and III). For WT, L-Y50F, and L-S93A, the enthalpy changes under the crystallization buffer conditions were almost the same as those under the phosphate buffer conditions, indicating that an increase in entropy loss led to a significant reduction of affinity. Additives such as polyethylene glycol and glycerol are known to reduce water activity and thus stabilize proteins by stabilizing the exposed hydrophobic regions of proteins (56, 57). These results highlight, therefore, the entropic contribution of dehydration upon complexation to the affinity of an antibody for the antigen. In contrast, the significant reduction of affinity of L-S91A for the target originated from a decrease in the negative enthalpy change (Tables II and III), suggesting that mutation may induce structural changes in the antigen-free antibody molecules, including solvated structures. We now discuss the role of hydrogen bond formation in the HyHEL-10-HEL interaction on the basis of the crystal structures of the mutant Fv-HEL complexes.
Comparison of the Mutant Fv-HEL Structure with the Wild-type Fv-HEL Structure-- Mutations did not affect the crystal packing. The overall structures of the mutant Fv-HEL complexes are similar to the structure of the wild-type Fv-HEL complex (Fig. 5). The area of HEL covered by the mutant Fvs was slightly decreased by 1-29 Å2 in comparison with the wild-type Fv-HEL complex (Table IV), indicating that almost the same areas are buried upon complexation. In addition, ~90% of the water molecules in the structures of the mutant Fv-HEL complexes are identical to those in the wild-type complex. Therefore, the local structural changes because of the mutations, including interface solvents, can be discussed directly by comparing the mutant structures with the wild-type structure.
The overall structures of the L-Y50F and L-S93A
mutant Fv-HEL complexes are almost identical to the structure of the
wild-type Fv-HEL complex, including the interfacial areas, the number
of noncovalent interactions, the location of interfacial solvent molecules, and the local structure around the mutated site. These results indicate that changes in the thermodynamic parameters (e.g. the Gibbs energy and the enthalpy changes) reflect the
effect of truncating the hydrogen bonds on the interactions. Removal of
the hydroxyl group from Tyr50 in the light chain resulted
in a significant reduction of the negative enthalpy change, and the
overall and local structures are almost identical to the wild-type
structure except for truncation of hydrogen bonds, suggesting that the
decrease in the negative enthalpy change (about 25 kJ
mol1) originated from deletion of two hydrogen bonds. The
side chain at site 50 of VL is almost buried (the calculated accessible
surface area is 3 Å2) in the complex, which might lead to
a significant reduction of the negative enthalpy change because of the
mutation. However, a smaller reduction in the Gibbs energy change,
originating from a smaller negative entropy change, was observed.
Wilcox et al. (58) and Murphy et al. (59)
suggested that the unfavorable entropy change results, in part, from
reduction of the conformational flexibility in the antibody upon
complexation. In the case of the interaction between Y82F mutant
FK506-binding protein (FKBP-12) and its ligand (FK506), large increases
in negative enthalpy and negative entropy change have been observed in
comparison with the wild-type FKBP-12 ligand interaction (60). A
crystallographic study of the complex formed between the wild-type
FKBP-12 and its ligands has indicated that two water molecules are
located in the hydroxyl group of Tyr82 in unliganded
FKBP-12 and that on ligand binding these water molecules are lost and
the hydroxyl group forms a hydrogen bond with the ligand. From these
experimental data, it has been suggested that the formation of a
hydrogen bond with the ligands results in a large decrease of negative
entropy change because of removal of the water molecules (60).
In contrast, the values of the thermodynamic parameters of the interaction between the L-S93A mutant Fv and HEL are smaller than those of the other mutants. The side chain of L-Ser93 is partially exposed, and thus, the effect of the mutation on the interaction might be smaller than in the case of the other two mutations.
Substitution of Ser91 with Ala resulted in the appearance
of a new water molecule at the mutated site in the mutant Fv-HEL
complex. The electron density map confirmed that this water molecule,
not found in the wild-type complex, was located near the mutated site, suggesting that the solvent compensated for removal of the hydroxyl group at site 91 of VL. Two water molecules in the wild type-HEL complex were retained in the mutant complex, and these water molecules formed hydrogen bonds with the hydroxyl group of Tyr20 of
HEL and with L-Asn32 and
L-His34 of Fv, bridging HEL with the Fv (Fig.
7C). One hydrogen bond was lost because of the mutation, and
three new hydrogen bonds were formed. Thus, the number of hydrogen
bonds in the mutant complex increased. However, the mutation resulted
in a reduction of the negative enthalpy change. These changes in the
thermodynamic parameters have been described for other antigen-antibody
interactions (e.g. the D1.3-HEL interaction (4, 61)) and for
OppA-peptide interactions (17, 18). Recently, we reported the effect of deleting side chains participating in salt bridge formation on the
HyHEL-10-HEL interaction (38). The newly introduced water molecules
that were observed around the mutated sites led to the formation of
several hydrogen bonds. Thermodynamic analyses of the salt
bridge-deleted mutant Fv-HEL interactions showed a significant increase
in the negative enthalpy changes because of the formation of the
hydrogen bonds (38). Taken together, these results suggest that the new
water molecule in the L-S91A-HEL complex might not be newly
introduced upon complexation (i.e. from bulk solvent water);
rather the ligand-free antibody or antibody-free antigen might be
involved in the formation of hydrogen bonds. As described above, the
number of hydrogen bonds via water molecules is increased; however,
less favorable H and more favorable
S have
been observed when an additional water molecule is found to bind in the
interface. One possible explanation for this would be that the presence
of the water molecule in the unliganded protein will reduce the
entropic cost of ligand binding, as adequately discussed in OppA system (18).
The most useful tie between the thermodynamic and structural data is
based on the relationship between the change in heat capacity and the
change in the surface area that is exposed to solvent on going from one
equilibrium state to another (62). For example, Spolar and Record (63)
successfully related the thermodynamic and structural changes on
formation of a protein-DNA complex. Thus, from the temperature
dependence of the enthalpy changes (Figs. 4 and 5), we estimated the
heat capacity changes (Cp) of the interactions
between the antibodies and the antigen (Tables II and III). We
calculated the
Cp values on the basis of
structural data and the equation,
Cp = 1.34
ASAapolar
0.59
ASApolar, reported
by Spolar and Record (63) (Table V). The
data clearly indicate a large discrepancy between the calculated values
and the experimentally determined values. The discrepancy was first
pointed out by Ladbury et al. (64). Possible explanations
for this discrepancy are restriction of water molecules in the
interface (62-65) and recognition-coupled structural changes because
of induced fitting (63, 66). Precisely estimating the contribution of
water molecules and structural changes is difficult; nonetheless,
significant contributions of larger negative
Cp
values may originate from interfacial water molecules, because no major
conformational changes have been observed in the antigens and/or in the
antibodies (6, 25-28, 67).
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Role of Hydrogen Bond Formation via Interfacial Water Molecules in the HyHEL-10-HEL Interaction: Conclusion-- Hydrogen bond formation via interfacial water molecules has been observed in various biomolecular interactions, and significant contributions of the hydrogen bonds to the interaction has been proposed (6, 7). Here, we have shown that the hydrogen bonds in the HyHEL-10-HEL complex are either buried (L-Tyr50 and L-Ser91) or partially exposed (L-Ser93); deletion of these hydrogen bonds led to thermodynamic compensation because little structural change was introduced by the mutations. We conclude that hydrogen bond formation via interfacial water molecules makes an enthalpic contribution to the interaction despite a partial offset because of an unfavorable entropy loss. As shown previously in several biomolecular interactions, the favorable enthalpy changes are compensated for by an unfavorable entropy change (68-70), suggesting that the antigen-antibody interaction tolerates truncation of these hydrogen bonds. It has been suggested that the unfavorable entropy change results, in part, from reduction of the conformational flexibility in the antibody upon complexation (71, 72), suggesting that the hydrogen bonds stiffen the antigen-antibody complex.
Finally, we note that almost all of the interfacial water molecules
were observed in the same positions as in the wild-type complex (72),
despite truncation of some of the residues participating in hydrogen
bond formation via interfacial water molecules. These results suggest
that interfacial water molecules might make a significant contribution
to the interaction through other factors, e.g. structural
stabilization of the antigen, the antibody, or its complex with a
smaller energetic contribution. Constant positioning of the water
molecules with different bound ligands has also been described
for the OppA-peptide system because of the fact that these sites are at
positions of lowest energy for occupancy of water (17). Computational
studies of the binding free energies based on the results reported here
may provide a more precise description of the role of hydrogen bond
formation via interfacial water molecules (73, 74).
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ACKNOWLEDGEMENTS |
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We thank N. Sakabe, S. Wakatsuki, M. Suzuki, and N. Igarashi of the Photon Factory for kind help with data collection. We also thank Dr. K. Maenaka of the National Institute of Genetics (Mishima, Japan) for valuable comments.
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FOOTNOTES |
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* This work was supported in part by grants-in-aid (to K. T. and I. K.) and fellowships for Japanese Junior Scientists (to M. S.) from the Japan Society for the Promotion of Science, and grants-in-aid for Priority Areas from the Ministry of Education, Science, Sports, and Culture of Japan and through the Proposal-based R&D Promotion Program and the Industrial Technology Research Grant Program in 2000 from the New Energy and Industrial Technology Development Organization (NEDO) of Japan.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The atomic coordinates and the structure factors (code 1J1O, 1J1P, and 1J1X for L-Y50F, L-S91A, and L-S93A, respectively) have been deposited in the Protein Data Bank, Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, NJ (http://www.rcsb.org/).
To whom correspondence may be addressed. Fax: 81-22-217-7276;
E-mail: tsumoto@mail.cc.tohoku.ac.jp.
¶ To whom correspondence may be addressed. Fax: 81-22-217-6164; E-mail: kmiz@mail.cc.tohoku.ac.jp.
Published, JBC Papers in Press, November 19, 2002, DOI 10.1074/jbc.M210182200
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ABBREVIATIONS |
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The abbreviations used are: HEL, hen egg white lysozyme; VH, variable region of immunoglobulin heavy chain; VL, variable region of immunoglobulin light chain; Fv, fragment of immunoglobulin variable regions; Fab, antigen binding fragment of immunoglobulin; WT, wild-type; L-Y50F mutant, mutant HyHEL-10 Fv in which Tyr50 of the VL chain is substituted with Phe; ITC, isothermal titration calorimetry; CDR, complementarity determining region.
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