Glutamate-induced Assembly of Bacterial Cell Division Protein FtsZ*

Tushar K. BeuriaDagger, Shyam Sundar Krishnakumar, Saurabh Sahar, Neera Singh, Kamlesh Gupta, Mallika Meshram, and Dulal Panda§

From the School of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, Mumbai 400 076, India

Received for publication, June 11, 2002, and in revised form, October 30, 2002

    ABSTRACT
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The polymerization of FtsZ is a finely regulated process that plays an essential role in the bacterial cell division process. However, only a few modulators of FtsZ polymerization are known. We identified monosodium glutamate as a potent inducer of FtsZ polymerization. In the presence of GTP, glutamate enhanced the rate and extent of polymerization of FtsZ in a concentration-dependent manner; ~90% of the protein was sedimented as polymer in the presence of 1 M glutamate. Electron micrographs of glutamate-induced polymers showed large filamentous structures with extensive bundling. Furthermore, glutamate strongly stabilized the polymers against dilution-induced disassembly, and it decreased the GTPase activity of FtsZ. Calcium induced FtsZ polymerization and bundling of FtsZ polymers; interestingly, although 1 M glutamate produced a larger light-scattering signal than produced by 10 mM calcium, the amount of polymer sedimented in the presence of 1 M glutamate and 10 mM calcium was similar. Thus, the increased light scattering in the presence of glutamate must be due to its ability to induce more extensive bundling of FtsZ polymers than calcium. The data suggest that calcium and glutamate might induce FtsZ polymerization by different mechanisms.

    INTRODUCTION
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

FtsZ, a 40.3-kDa protein, is a key protein involved in prokaryotic cell division (1, 2). It forms the structural element called Z-ring at the site of cell division, and it remains associated with the inner face of the cytoplasmic membrane throughout the septation process (3). Homologs of FtsZ have been found in every free-living prokaryote examined to date (4-6), including many species of Archaea. It has also been found in chloroplasts of higher plants, where it is involved in chloroplast division (7). Its wide distribution and high degree of sequence conservation suggest that it probably plays a similar role in all bacterial and archaeal species (8-10).

Consistent with its cytoskeletal role, FtsZ has several properties in common with the eukaryotic cytoskeleton protein, tubulin. Like tubulin, FtsZ binds and hydrolyzes GTP, and it polymerizes to form long tubules in a GTP-dependent manner (11-14). It has been shown that purified FtsZ polymerizes into structures that closely resemble those formed by purified tubulin (15). Furthermore, FtsZ shows limited but significant sequence similarity to tubulin. Sequence alignment of FtsZ and tubulin reveals that several structural glycine and proline residues and most of the residues that are involved in GTP binding are conserved. In addition, FtsZ contains the tubulin signature GTP binding sequence motif GGGTG(T/S)G (11, 12, 16). Recently, the crystal structure of FtsZ from Methanococcus jannaschii has been determined (17). FtsZ has two domains arranged around a central helix. GTPase activity is localized in the amino terminus domain of the protein, whereas the carboxyl-terminal domain function is still unknown (17). The three-dimensional structure of FtsZ also shows striking similarities with alpha  and beta  tubulin (18). Furthermore, molecular phylogenetic data indicate that an archaeal FtsZ is the most tubulin-like of all prokaryotic FtsZ proteins found so far, suggesting that tubulin may have evolved from an ancestral FtsZ (5).

At low concentrations, purified FtsZ monomers polymerize into single-stranded protofilaments with little or no bundling of protofilaments in an assembly reaction that is believed to be isodesmic (non-cooperative) in nature (19). However, in the presence of DEAE-dextran (15, 20) or high concentrations of calcium (21-23) FtsZ monomers polymerize into long, rod-shaped or tubular polymers that become extensively bundled. The assembly reaction is stimulated, and resulting polymers are stabilized by these agents. These studies demonstrate that the extent and nature of the polymer formed highly depends on the reaction conditions. Interestingly, calcium exerts contrasting effects on the polymerization of FtsZ and microtubules. Calcium strongly inhibits tubulin assembly and depolymerizes microtubules, whereas it promotes FtsZ assembly and induces bundling of FtsZ filaments (23). Biochemical studies have been carried out to study the effect of various factors such as temperature and pH on FtsZ polymerization (24-26). Many inducers of tubulin polymerization have been identified over the years, and these modulators of polymerization have been extensively utilized to understand the molecular mechanism of microtubule polymerization dynamics (27); however, only a few inducers of FtsZ polymerization have thus far been identified.

In this study we found that monosodium glutamate is a potent inducer of FtsZ polymerization, and it stabilizes FtsZ polymers against disassembly. Electron micrographs showed that glutamate-induced FtsZ polymers form extensive bundles. Furthermore, glutamate decreased the GTPase activity of FtsZ. The property of filament bundling may be important in FtsZ function in the septation process. In addition, calcium and glutamate exerted differential effects on FtsZ polymerization, indicating that they modulate FtsZ polymerization through different mechanisms.

    EXPERIMENTAL PROCEDURES
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
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DISCUSSION
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Materials-- Pipes,1 monosodium glutamate, isopropyl-beta -D-thiogalactopyranoside, bovine serum albumin, and GTP were obtained from Sigma. DE-52 was from Whatman International Ltd. All other chemicals used were of analytical grade.

Purification of FtsZ-- Recombinant Escherichia coli FtsZ was overexpressed and purified from BL21 strain (a gift from Dr. H. P. Erickson, Duke University) as described earlier (28) with some modifications. Briefly, the cells were grown at 37 °C in LB broth containing 50 µg/ml ampicillin until they reached early log phase (A600 ~ 0.4-0.6) when protein expression was induced by the addition of 0.4 mM isopropyl-beta -D-thiogalactopyranoside for an additional 5 h. Cells were harvested by centrifugation at 12,000 × g at 4 °C for 30 min and suspended in lysis buffer (50 mM Tris (pH 8), 100 mM NaCl, 1 mM EDTA, 1 mM phenylmethylsulfonyl fluoride, 0.1% beta -mercaptoethanol, and 1 mM MgSO4) on ice. Cells were lysed by sonication with 20-s pulses at 30-s intervals between the pulses for four cycles. Partially lysed cells were further disrupted using a Polytron homogenizer (Polytron PT-3000). Lysozyme (0.4 mg/ml) was added to the partially lysed cell suspension, which then was incubated on ice for 1 h. It was again incubated with 10 mM MgSO4 for 5 min, and the suspension was sonicated for 6 cycles with 20-s pulses. The insoluble debris was removed by centrifugation at 12,000 × g at 4 °C for 30 min. The cell-free supernatant was saturated to 25% with ammonium sulfate solution and incubated for 90 min at 4 °C. The protein suspension was centrifuged at 27,000 × g at 4 °C for 30 min. The pellet was resuspended in 50 mM Tris buffer (pH 8) and dialyzed against the same buffer for 4 h. Purification of FtsZ was further carried out using DE-52 ion exchange column chromatography as described previously (13). Briefly, FtsZ was loaded on a DE-52 column pre-equilibrated with 50 mM Tris buffer (pH 8), and the protein was eluted with increasing concentrations of KCl. FtsZ-containing fractions were eluted at 250 mM KCl; protein concentrations were determined by the Bradford method (29). These fractions were pooled together and concentrated using Amicon Centriplus concentrators at 4 °C. One cycle of temperature-dependent polymerization and depolymerization was then used to further purify the FtsZ. The polymerization reaction was carried out in buffer containing 25 mM Pipes (pH 6.8), 10 mM MgSO4, 10 mM CaCl2, 1 M monosodium glutamate, and 1 mM GTP at 37 °C for 30 min. FtsZ polymers were sedimented by centrifugation at 27,000 × g for 30 min at 27 °C. The pellet was resuspended on ice in 25 mM Pipes buffer (pH 6.8), and the suspension was centrifuged at 12,000 × g for 15 min at 4 °C to remove the insoluble aggregates. The FtsZ concentration was determined by the Bradford method using bovine serum albumin as a standard (29), and FtsZ was frozen and stored at -80 °C.

Light-scattering Assay-- FtsZ (6 µM) was prepared in 25 mM Pipes buffer (pH 6.8) and 3 mM MgSO4 (buffer A) in the absence and presence of different concentrations of monosodium glutamate on ice. After the addition of 1 mM GTP, the sample was immediately placed in a cuvette at 37 °C, and the polymerization reaction was monitored at 37 °C by light scattering at 500 nm using a JASCO 6500 spectrofluorometer. The excitation wavelength was 500 nm.

Sedimentation Assay-- FtsZ (6 µM) was polymerized in buffer A containing 1 mM GTP in the absence and presence of different concentrations of sodium glutamate for 30 min at 37 °C. The polymers were collected by sedimenting them at 27,000 × g for 30 min at 27 °C. The protein concentration in the supernatant was measured by the Bradford assay using bovine serum albumin as a standard.

Electron Microscopy-- FtsZ (6 µM) was polymerized at 37 °C in buffer A with 1 mM GTP and in the absence or presence of 1 M glutamate for 30 min. The FtsZ polymers in the samples were fixed with prewarmed 0.5% glutaraldehyde for 5 min. Twenty-microliter volumes of protein samples were transferred onto carbon-coated copper grids (300 mesh) and blotted dry. The grids were subsequently negatively stained with a few drops of 2% uranyl acetate solution and air-dried. The samples were examined using a JEOL-JEM 100S or a Philips CM 200 electron microscope.

Stability of FtsZ Polymers in the Presence of Glutamate-- FtsZ (1 mg/ml) was polymerized in buffer A with 10 mM calcium and 1 mM GTP at 37 °C for 30 min. The protein solution was then diluted 20 times to reach a final FtsZ concentration of 0.05 mg/ml in different concentrations of warm glutamate. The reaction mixtures were incubated at 37 °C for another 30 min and centrifuged at 50,000 × g for 30 min. The pellet obtained were dissolved in 30 µl of 25 mM Pipes buffer containing 0.5% SDS, and 20 µl of each sample was analyzed by 10% SDS-PAGE. The intensity of the bands was determined by a gel documentation system obtained from Kodak Digital Science.

GTP Hydrolysis-- A malachite green sodium molybdate assay was used to measure the production of inorganic phosphate during GTP hydrolysis (19, 30). Samples containing 6 µM FtsZ with different concentrations of glutamate were prepared in buffer A at 0 °C, and 1 mM GTP was added to the reaction mixtures. Immediately, the reaction mixtures were transferred to 37 °C and incubated for different lengths of time. The hydrolysis reaction was quenched at desired time points by the addition of 10% v/v 7 M perchloric acid to the reaction mixtures, and the quenched reaction mixtures were centrifuged for 5 min to remove aggregated proteins. Twenty microliters of the supernatants were incubated with 900 µl of filtered malachite green solution (0.045% malachite green, 4.2% ammonium molybdate, and 0.02% Triton X-100) at room temperature for 30 min, and the phosphate ions produced were determined by measuring the absorbance of samples at 650 nm. The reaction was normalized including a control without FtsZ. A phosphate standard curve was prepared using sodium phosphate.

    RESULTS
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Purification of FtsZ by Glutamate-enhanced Polymerization-- FtsZ and its eukaryotic homolog tubulin are both known to undergo multiple temperature-dependent polymerization and depolymerization cycles in the presence of GTP (31). The polymerization of tubulin is well understood, and several inducers of tubulin assembly in vitro are known. The purification procedure for tubulin involves temperature- and GTP-dependent polymerization and depolymerization cycles followed by phosphocellulose chromatography, which not only increases the purity level of the protein but also increases the yield of the assembly-competent fraction of tubulin (32, 33). Taking advantage of the tubulin literature, we sought to find inducers of FtsZ polymerization. We tried several well known agents that promote tubulin polymerization including dimethyl sulfoxide (Me2SO), glycerol, taxol, and glutamate (34-36). In contrast to their effects on tubulin polymerization, 4 M glycerol, 10% v/v Me2SO, and 10 µM taxol did not show any effect on FtsZ polymerization (data not shown). However, glutamate did enhance FtsZ assembly in a concentration range similar to the concentration range that enhances tubulin polymerization (Fig. 1). Several groups have routinely purified FtsZ from crude extracts using a cation exchange (DE-52) chromatographic procedure (13, 28). Thus, similar to the purification procedure used for tubulin, we introduced a glutamate-induced temperature-dependent polymerization and depolymerization cycle after the DE-52 column to further purify FtsZ while keeping its activity intact ("Experimental Procedures"). The combination of a cycle of polymerization and depolymerization with DE-52 chromatography yielded FtsZ of high purity (>98%) as analyzed by Coomassie-stained SDS-PAGE (data not shown).


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Fig. 1.   Glutamate-induced polymerization of FtsZ. A, FtsZ (6 µM) was polymerized in buffer A containing 1 mM GTP at 37 °C, in the absence (diamond ) and presence of 0.25 (black-triangle), 0.5 (open circle ), 0.75 (), and 1 M () glutamate. The rate and extent of the polymerization reaction were measured by light scattering at 500 nm using a fluorescence spectrophotometer. The light-scattering signals were corrected by subtracting appropriate blank scans. B, FtsZ (8 µM) was polymerized in buffer A containing 1 mM GTP at 37 °C for 10 min. Then 1 M glutamate was added to the cuvette, and the polymerization of FtsZ was monitored for an additional 15 min. The glutamate solution was added from a stock solution of 2 M glutamate, which reduced the concentration of FtsZ to 4 µM. The light-scattering data were corrected with respect to buffer A and 1 M glutamate.

Glutamate-induced Polymerization of FtsZ-- Our goal was to discover modulators of FtsZ polymerization, which could be further used to understand the basic mechanisms of FtsZ polymerization and the roles of FtsZ polymerization in bacterial cell division. Monosodium glutamate increased the rate and extent of FtsZ polymerization in a concentration-dependant manner with an optimal effect at a concentration of 1 M (Fig. 1A). To further demonstrate that glutamate is a potent inducer of FtsZ polymerization, the rate and extent of FtsZ (8 µM) polymerization was first monitored for 10 min by light scattering in the absence of glutamate. After 10 min, 1 M glutamate was added to the cuvette from a stock of 2 M warm glutamate, and the polymerization reaction was monitored for an additional 15 min. The addition of 1 M warm glutamate strikingly increased the magnitude of the light-scattering signal, and an apparent steady state was achieved quickly (Fig. 1B).

To verify whether the increase in the light-scattering signal was due to a genuine increase in the FtsZ polymerization, we measured the mass of polymeric FtsZ in the presence of different concentrations of glutamate (Fig. 2). The FtsZ polymer mass clearly increased with increasing concentrations of glutamate. Between 85 and 92% of the initial protein could be sedimented as polymer mass in the presence of 1 M glutamate. To find out whether glutamate-induced FtsZ polymerization was temperature-dependent or not, 6 µM FtsZ was incubated with 1 M glutamate plus 1 mM GTP in buffer A for 30 min at 4 °C. After incubation, the reaction mixture was centrifuged at 4 °C as previously described in Fig. 2. Then the protein concentration of the supernatant was measured, and it was found to be identical with the total protein concentration used in the assay (data not shown). The result showed that no glutamate-induced polymerization occurred at 4 °C. However, ~90% of the total protein formed sedimentable polymers at 37 °C, indicating that the polymerization reaction is temperature-dependent.


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Fig. 2.   Determination of polymer mass by a sedimentation assay. FtsZ (6 µM) was polymerized in buffer A containing 1 mM GTP in the absence and presence of different concentrations of glutamate at 37 °C for 30 min and sedimented at 27,000 × g at 25 °C for 45 min. The protein concentrations of the supernatants were determined by the Bradford method. The protein concentrations of the pelleted fractions were determined by subtracting the supernatant concentrations from the total protein concentrations. Data were averages of three independent experiments.

Electron micrographic analysis of the FtsZ polymer showed formation of a dense network of FtsZ polymers in the presence of M glutamate (Fig. 3, B and C), whereas only a few polymeric filamentous structures were formed in the absence of glutamate (Fig. 3, A and D). Furthermore, analysis of the electron micrographs showed that sodium glutamate not only increased the amount of polymer but also induced formation of large filamentous polymeric structures with extensive bundle formations (Fig. 3, E and F).


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Fig. 3.   Electron micrographs of FtsZ polymers. FtsZ (6 µM) was polymerized in buffer A in the absence or presence of 1 M glutamate at 37 °C. The samples were prepared for electron microscopy as described under "Experimental Procedures." A (×10,000) and D (×28,000) show FtsZ polymers in the absence of glutamate and B and C (×10,000) and E and F (×28,000) show FtsZ polymers formed in the presence of 1 M glutamate.

Does Glutamate Stabilize FtsZ Polymers against Dilution-induced Disassembly?-- We wanted to determine whether glutamate could stabilize FtsZ polymers against dilution-induced disassembly. FtsZ (1 mg/ml) was initially polymerized with 10 mM calcium in the presence of 1 mM GTP. The polymerized FtsZ was diluted 20 times to a final concentration of 0.05 mg/ml FtsZ into buffers containing different concentrations of warm monosodium glutamate at 37 °C. After 30 min of incubation, FtsZ polymers were sedimented, and the protein concentrations in the pellets were quantified using Coomassie-stained SDS-PAGE (Fig. 4, A and B), which clearly showed that the amount of polymeric protein increased with increasing glutamate concentration. For example, the polymer content increased by 9.2-fold in the presence of 1 M glutamate as compared with the control value. Thus, the data indicated that glutamate stabilizes FtsZ polymers and prevents their dilution-induced disassembly.


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Fig. 4.   Stabilization of FtsZ polymers against dilution-induced disassembly by glutamate. FtsZ (1 mg/ml) was polymerized in the presence of 10 mM calcium and 1 mM GTP for 30 min. Polymerized FtsZ was diluted 20 times into buffer A containing different concentrations (0, 0.25, 0.5, 0.75, and 1 M) of glutamate at 37 °C. Samples were centrifuged, dissolved, and loaded on a 10% SDS-PAGE as described under "Experimental Procedures." A, the intensities of the bands were determined by a gel documentation system from Kodak Digital Science, and relative protein concentrations of these bands were determined with respect to control. The data were plotted against glutamate concentrations (B).

Glutamate Reduces the GTPase Activity of FtsZ-- FtsZ is a GTPase, and the polymerization of FtsZ is thought to be regulated by GTP hydrolysis (14, 37). Initially, we wanted to know whether GTP is essential for glutamate-induced FtsZ polymerization. The FtsZ polymerization reaction was carried out for 10 min in the presence of 1 M glutamate without GTP. There was no development of light scattering (Fig. 5A). After 10 min, 1 mM GTP was added to the cuvette, and the polymerization was immediately started, demonstrating that GTP is required for glutamate-induced polymerization of FtsZ. Similarly, GTP was also found to be essential for calcium-induced polymerization of FtsZ (Fig. 5A). Because GTP was found to be essential for the glutamate-induced assembly of FtsZ, we sought to determine the effects of glutamate on the GTPase activity of FtsZ. Monosodium glutamate decreased the rate of GTP hydrolysis in a concentration-dependent manner (Fig. 5B). For example, 1 M glutamate reduced the amount of hydrolyzed GTP of FtsZ by ~50%. Interestingly, GTP hydrolysis occurred at a significantly higher rate in the initial stage of the reaction than the late stage of the reaction (Table I). For example, in the absence of glutamate, 5 and 30 min of the hydrolysis reaction produced 69 ± 11 and 103 ± 8 mol of phosphate ions, respectively. However, in the presence of 1 M glutamate, 36 ± 7 and 56 ± 4 mol of phosphate ions were produced after 5 and 30 min of hydrolysis reaction (Table I). Glutamate stabilized the FtsZ polymers (Fig. 4, A and B) and induced an extensive bundling of FtsZ polymers (Fig. 3, E and F); thus, the reduction in the GTPase activity of FtsZ polymers by monosodium glutamate could be due to the enhanced stability of the FtsZ polymers.


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Fig. 5.   A, FtsZ (6 µM) was polymerized in buffer A in the presence of either 1 M glutamate (open circle ) or 10 mM CaCl2 () without GTP, and the assembly reaction was monitored for 10 min by light scattering 500 nm. After 10 min of reaction as marked by an arrow, 1 mM GTP was added to the cuvette, and the polymerization reaction was followed for an additional 10 min. B, effects of glutamate on the GTPase activity of FtsZ. FtsZ (6 µM) was mixed with different concentrations of glutamate on ice. After the addition of 1 mM GTP to the reaction mixtures, the sample solutions were immediately transferred to 37 °C and incubated for 5 min. The extent of GTP hydrolysis was measured as described under "Experimental Procedures." The data are averages of three experiments.

                              
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Table I
Effects of glutamate on the GTPase activity of FtsZ
The FtsZ concentration was 6 µM.

Comparison of the Effects of Glutamate and Calcium on FtsZ Polymerization-- Recently, it has been shown that high millimolar concentrations of calcium enhance the rate and the extent of FtsZ polymerization and promote the formation of tubulin-like protofilaments in the polymers (21, 22). Thus, it was interesting to compare the effects of glutamate and calcium on FtsZ polymerization. As previously reported, we also found that calcium induced polymerization of FtsZ, as evident from the increase in light scattering (Fig. 6A). However, glutamate (1 M) strikingly increased the magnitude of light scattering compared with 10 mM calcium (Fig. 6A). The increased light scattering by glutamate compared with calcium might be due to an increase in the polymer mass, to changes in the size and shape of the polymers, or to a combination of both the factors. To discern the possibilities, a sedimentation assay and electron microscopy were performed. We found that similar amounts of polymeric FtsZ were sedimented when the polymerization reaction was initiated by either 10 mM calcium or 1 M glutamate (Fig. 6B). Thus, the observed increase in the light-scattering intensity in the presence of glutamate was not due to an increase in the polymer mass. The electron micrographs of FtsZ polymers formed in the presence of 10 mM calcium are shown in Fig. 6C. These polymers are similar to previously reported calcium-induced FtsZ polymers (21). However, large filamentous structures containing thick bundles of FtsZ polymers were more abundant in the presence of 1 M glutamate (Fig. 3) compared with calcium (Fig. 6C). Therefore, the observed increase in light scattering in the presence of glutamate must be due to extensive bundling of FtsZ polymers.


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Fig. 6.   A, FtsZ (6 µM) was polymerized in the presence of either 10 mM CaCl2 (open circle ) or 1 M glutamate (black-triangle). The control polymerization reaction () was carried out in buffer A in the absence of both CaCl2 and glutamate. The polymerization reaction was monitored by light scattering at 500 nm. B, after 30 min of polymerization at 37 °C, FtsZ polymers were centrifuged at 50, 000 × g, and pellets were dissolved in 25 mM Pipes buffer containing 0.5% SDS. The protein content of the pellets were determined by Coomassie staining of a 10% SDS-PAGE. Lanes 1 and 2 show the Coomassie-stained gel photographs of FtsZ sedimented in the presence of 10 mM calcium or 1 M glutamate, respectively. C, electron micrographs of FtsZ polymers. FtsZ (6 µM) was polymerized in the buffer A in the presence of 10 mM calcium at 37 °C. The samples for electron microscopy were prepared as described under "Experimental Procedures," and the pictures were taken at ×27,500 magnifications.


    DISCUSSION
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
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DISCUSSION
REFERENCES

In this report we found that monosodium glutamate increased the rate and extent of FtsZ polymerization. It also enhanced the bundling of FtsZ filaments, and it stabilized the polymers against dilution-induced disassembly. At low protein concentrations, FtsZ assembly reaction is believed to be isodesmic (non-cooperative). Under these conditions, FtsZ monomers assemble into single-stranded polymers with few protofilament bundles (19). However, polycations such as DEAE-dextran and calcium have been shown to modify the morphology of FtsZ polymers. In the presence of DEAE-dextran, FtsZ protofilaments associate to form three-dimensional sheets (20, 38) and millimolar concentrations of calcium-pronounced bundling of FtsZ filaments have been observed by several groups (21-23). It was suggested that DEAE-dextran and calcium increase the lateral interactions between the protofilaments (20, 39).

Hydrophobic interactions play an important role in FtsZ assembly. It was proposed previously that drugs like bis-1-anilino-8-naphthalenesulfonate inhibits FtsZ polymerization, presumably by blocking intermolecular hydrophobic interactions between FtsZ molecules (40), whereas binding of calcium or DEAE dextran to FtsZ increases the intermolecular hydrophobic interactions, resulting in stimulation of FtsZ assembly (22, 38). In our experiments we found that the glutamate increased the rate and extent of the FtsZ polymerization to an optimal effect at a concentration of 1 M. At the optimal concentration of glutamate, FtsZ polymerization was biphasic, with a rapid burst phase of polymerization followed by a slower and prolonged linear phase (Fig. 1A). Electron micrographs of glutamate-enhanced FtsZ polymers showed large filamentous structures with extensive bundles (Fig. 3). These polymeric structures were somewhat similar to the FtsZ polymers formed in the presence of high concentrations of calcium (22); however, more bundles were present in the presence of glutamate compared with calcium. The results indicated that, like calcium, glutamate could increase the intermolecular hydrophobic interactions in FtsZ and stabilize the polymers by enhancing bundle formation. The slower phase of polymerization observed by light scattering could be due to the formation of the FtsZ polymer bundles. Furthermore, when preformed FtsZ polymer was diluted into different concentrations of glutamate, the polymers were found to be 9 times more stable in the presence of 1 M glutamate compared with no glutamate (Fig. 4). This shows that glutamate not only enhanced the FtsZ polymer formation but also stabilizes the preformed polymers.

Kinetics of nucleotide hydrolysis is important to describe the model for FtsZ polymerization. The active site for GTP hydrolysis in FtsZ is postulated to form when the T7 loop of a FtsZ monomer binds with the nucleotide-binding site on an adjacent FtsZ monomer (41). It was also thought that, like microtubules, FtsZ polymers could also be stabilized by a GTP cap (39). But recently Scheffers and Driessen (42) showed that GTP hydrolysis occurs immediately after the addition of GTP to FtsZ, and FtsZ polymers contain mostly GDP and inorganic phosphate rather than GTP (42). FtsZ assembly does not require magnesium or calcium, but magnesium is required for dynamic behavior of FtsZ polymers (43), whereas calcium at high concentrations induces the bundling of polymers, which reduces the dynamic behavior of FtsZ assembly (23). In the absence of magnesium, calcium inhibits GTP hydrolysis and reduces the dynamic nature of FtsZ polymers by making the polymers more stable. Our experiment showed that glutamate decreased the GTPase activity of FtsZ (Fig. 5). Glutamate might stabilize FtsZ polymers by increasing hydrophobic interactions between subunits and enhancing bundle formation, which decreased the dynamics of the polymers. Thus, the increased stability of FtsZ could reduce the rate of subunit exchange, which might be the cause of suppression of its GTPase activity. However, it is also difficult to rule out the possibility that the reduction in the GTPase activity of FtsZ by glutamate could also be responsible for higher stability of the polymers.

Calcium induces polymerization of FtsZ (Fig. 6 and Ref. 21). Interestingly, glutamate strikingly increased the rate and extent of the development of the light-scattering signal of FtsZ polymerization compared with calcium (Fig. 6A). However, the polymer mass was found to be similar in the presence of either glutamate or calcium. Electron micrographs of FtsZ polymers showed that glutamate caused extensive bundling of polymers compared with the degree of bundling caused by calcium, indicating that glutamate induces different conformational changes in the polymer than does calcium. Because 10 mM calcium alone was sufficient to polymerize most of the FtsZ, the increased intensity in the light scattering signal during FtsZ polymerization in the presence of 1 M glutamate must be due to the rearrangement of FtsZ polymers into thicker bundles rather than to an increase in polymer mass. The data indicate not only the presence of different modulator sites on FtsZ but also different mechanisms of FtsZ polymerization.

Tubulin, the eukaryotic homolog of FtsZ, is also known to form different types of polymers, including microtubules, open sheets (in the presence of zinc and Me2SO), double rings and isodesmic aggregates (in the presence of vinblastine), although only microtubules are responsible for the functional properties of tubulin (34, 44, 45). Interestingly, we found that the common inducers of tubulin assembly such as Me2SO, glycerol, and taxol had no effect on FtsZ polymerization (data not shown). It has been reported that high concentrations of glutamate stabilize tubulin and induce its polymerization. Glutamate increases the GTPase activity in tubulin and thereby enhances the rate of polymerization of tubulin into microtubules (36). In contrast, we found that glutamate strongly suppressed the GTPase activity of FtsZ. The binding of glutamate to tubulin is thought to induce localized opening or disordering of tubulin subunits without any major reorganization of the internal structure of tubulin (46). These data together indicate that glutamate interacts with FtsZ in a manner different from that of tubulin or microtubules.

FtsZ polymerizes to form a dynamic ring marking the division plane of prokaryotic cells and plays an essential role in cytokinesis (1, 3). Despite its actin-like role, FtsZ has structural and biochemical similarity to tubulin (5, 11, 15, 17). Tubulin, the eukaryotic homolog of FtsZ, has been successfully targeted for the development of anticancer and antifungal drugs (47-50). Thus, the potential use of FtsZ as a drug target for the development of antimicrobial agents needs to be explored. Several potent inhibitors of tubulin assembly including colchicine and vinblastine have no effect on FtsZ assembly, suggesting that these drugs do not interact with FtsZ (40). Interestingly, calcium depolymerizes microtubules (51), whereas it promotes the polymerization of FtsZ. Furthermore, glycerol, Me2SO and taxol are well characterized inducers of microtubule assembly, but these agents do not induce FtsZ assembly, suggesting that the polymerization reaction of FtsZ is mechanistically different from that of tubulin assembly. The finding of new modulators of FtsZ assembly-like glutamate would certainly help to characterize FtsZ polymerization and may help to search for new inhibitors. For example, a screen could be designed in which inhibitors of glutamate-induced polymerization might be identified.

    ACKNOWLEDGEMENTS

We thank Dr. H. P. Erickson for providing us the FtsZ clone. We thank Drs. Leslie Wilson, D. Dasgupta, and S. Pathare for critical reading of the manuscript. We thank the Regional Sophisticated Instrumentation Centre, IIT, Bombay and the Tata Institute of Fundamental Research, Bombay for allowing us to use their electron microscopy facilities.

    FOOTNOTES

* The study was funded by a grant (to D. P.) from the Department of Science and Technology, Government of India.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Supported by fellowship from Council of Scientific and Industrial Research, Government of India.

§ To whom correspondence should be addressed. Tel.: 91-22-572-2545 (ext. 7838); Fax: 91-22-572-3480; E-mail: panda@btc.iitb.ac.in.

Published, JBC Papers in Press, November 22, 2002, DOI 10.1074/jbc.M205760200

    ABBREVIATIONS

The abbreviation used is: Pipes, piperazine-N,N'-bis(2-ethanesulfonic acid).

    REFERENCES
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

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