Polyamines and Glutamate Decarboxylase-based Acid Resistance in Escherichia coli*

Il Lae Jung and In Gyu Kim {ddagger}

From the Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P. O. Box 105, Yusong, Taejon 305-600, Korea

Received for publication, November 26, 2002


    ABSTRACT
 TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
The expression of gadA and gadB, which encode two glutamate decarboxylases (GADs) of Escherichia coli, is induced by an acidic environment and participate in acid resistance. In this study, we constructed a polyamine-deficient mutant and investigated the role of polyamines in acid resistance. The expression of gadA and gadB was shown to be dependent on polyamines. For that reason, the polyamine-deficient mutant was completely devoid of GAD activity and was very susceptible to low pH if large amounts of polyamines were not provided. We also showed that the polyamine-deficient mutant contained higher cAMP levels than the isogenic polyamine-proficient wild type, and cAMP negatively regulated the expression of gadA and gadB. Therefore, introduction of the cya (encoding adenylate cyclase) mutation allele into the polyamine-deficient mutant resulted in the increment of GAD activity and thus restored the reduced acid resistance of the mutant. The positive regulators, H-NS (histone-like protein, encoded by the hns gene) and RpoS (alternative RNA polymerase {sigma} subunit, encoded by rpoS gene), also significantly governed the expression of gadA and gadB, respectively. However, polyamines did not regulate either the intracellular H-NS level or rpoS expression under these culture conditions. These results strongly suggest that there are at least two different regulatory systems in acid resistance, one is positive regulation via a H-NS/RpoS system and the other is negative regulation via a polyamine/cAMP system.


    INTRODUCTION
 TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
Escherichia coli maintains its internal pH between 7.4 and 7.8 during aerobic growth under an external pH of 5.0–9.0 (33); however, the mechanisms of pH homeostasis remain elusive (19). The ability to survive in an acidic environment is essential for successful colonization of the mammalian host by both commensal and pathogenic enteric bacteria (26). In order to survive in the mammalian host, commensal and pathogenic bacteria must have specific mechanisms that can overcome the deleterious effect of the acidic environment, because these microorganisms are faced with acidic shock (pH < 2.5) during their passage through the stomach (26). Numerous strategies including amino acid decarboxylase-based systems are necessary to enable them to combat this acidic stress (11, 26).

The glutamate decarboxylase (GAD,1 EC 4.1.1.15 [EC] ) system has been extensively studied because of its major role in the acid resistance of enteric pathogens such as E. coli O157, Shigella flexneri, and Listeria monocytogenes (6, 7, 8, 29). E. coli contains two genes, gadA and gadB (mapped at 78 and 33 min on its chromosome), which encode two biochemically indistinguishable forms of GAD and are very similar in sequence (2, 9, 24). Sequential reaction through GAD and the putative glutamate/GABA antiporter encoded by the gadC gene allows cells to remove intracellular protons (8).

The expression of gad genes is induced at the stationary phase under normal aerobic conditions and positively responds to acidic, hyper-, and hypo-osmotic shocks (8). The extent of gadA and gadB expression can be differentiated depending on the culture conditions, and both the stationary phase and acidic pH activate distinct regulatory circuits (8). Two distinct regulatory proteins, H-NS (histone-like protein, encoded by hns) and RpoS (alternative RNA polymerase {sigma} subunit, encoded by rpoS), are known to be involved in the regulation of gadA and gadB expression. The expression of gadA and gadB is negatively regulated by H-NS during the exponential growth phase, while stationary phase induction of gadA and gadB expression requires sigma factor RpoS (sigma S) (5, 6, 8). Another regulatory mechanism via the action of cAMP receptor protein (CRP) has been suggested, in which CRP represses gad expression (5).

Polyamines, the ubiquitous amine-containing molecules, have various important physiological roles (32). They participate in many cellular processes including modulation of gene expression, signal transduction, protein synthesis, regulation of cell growth and differentiation, and an oxidative defense mechanism (13, 16, 18, 22, 25, 31). Despite their biological importance, the participation of polyamines in acid resistance has been rarely studied to date.

The present work was undertaken to identify the environmental and regulatory factors affecting the expression of gad genes in E. coli. Some evidence that polyamine is essential for the normal expression of gad genes. It is suggested that both H-NS and RpoS are "positively" involved in gad expression as transcriptional regulators. We also suggest that the polyamine/cAMP system as well as the H-NS/RpoS system plays an important part in acid resistance through the regulation of the intracellular cAMP concentration by polyamines. Finally, the possible regulatory circuits for acid resistance via a GAD system are presented.


    EXPERIMENTAL PROCEDURES
 TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
Bacterial Strains and Growth Conditions—Bacterial strains used in this study are shown in Table I. All strains are E. coli K-12 MG1655 [{lambda}-, rph-] derivatives. To construct a polyamine-deficient mutant strain, {Delta}(speA-speB) and {Delta}(speC-glc) mutations were simultaneously transduced into QC2461 with selection of the linked galP::Tn10 as described by Minton et al. (22), and the polyamine concentrations of the strain were determined (Table II). A total of 41 of 172 transductants did not grow in a minimal M9 medium containing 10 mM glycolic acid as a sole carbon source, and only 3 of them grew normally when supplemented with putrescine or spermidine (co-transduction frequency was about 1.74%, see Ref. 12). The polyamine concentrations of those 3 strains were determined, and one of the resultant polyamine-deficient mutant strains was designated as JIL585. To construct a Tc-s derivative (JIL601) of JIL585, a fusaric acid selection method was carried out as described by Maloy and Nunn (20).


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TABLE I
Bacterial strains used in this study

 

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TABLE II
Intracellular polyamine concentrations

 

The gad::lacZ fusions and various mutations were introduced into strains of interest using P1-mediated transduction (21). In vivo recombination between a plasmid carrying rpoS742::lacZ transcription fusion and {lambda}RZ5 was used to transfer the fusion to bacteriophage lambda. {lambda}(rpoS742::lacZ) was produced by growing {lambda}RZ5 on the strain carrying plasmid pRL45. The resultant lysate was used to infect JIL601, and desirable lysogen was selected as a blue colony on a lactose (0.4%) minimal medium with 20 µg/ml 5-bromo-3-indolyl-{beta}-D-galactopyranoside (X-gal) and 100 µg/ml ampicillin.

To transduce cya (encoding adenylate cyclase) mutation allele in the gadA or gadB::lacZ fusion strain (both cya and gadA::lacZ or gadB::lacZ fusion have the kanamycin-resistant gene), transductants were isolated in LB containing phosphomycin (30 µg/ml) as previously described (27). The addition of cAMP restored sensitivity to the phosphomycin.

Cells were grown in an LB (Bacto tryptone, 10 g; Bacto yeast extract, 5 g; NaCl, 10 g per liter) broth or a minimal M9 medium supplemented with 0.4% glucose as a carbon source. Thiamine (1 µM) was added to all minimal M9 medium used in this study. Overnight cultures grown in LB were washed and diluted to an initial OD600 of 0.01 in a minimal M9 medium containing 0.4% glucose as the sole carbon source and cultivated again overnight. These overnight cultures were diluted to an OD600 of 0.01 in the same fresh medium and incubated for the times indicated. Kanamycin (km), tetracycline (Tc), ampicillin (amp), and chloramphenicol (cm) were added to the medium when needed, to reach final concentrations of 75 µg/ml, 20 µg/ml, 100 µg/ml, and 30 µg/ml, respectively. Tryptophan was added at a final concentration of 25 µg/ml for the growth of strains containing the gad::lacZ fusion.

Measurement of Polyamine Contents—Putrescine and spermidine concentrations were determined by high performance liquid chromatography as described previously (15). The stationary phase-grown cultures (1 x 1010 cells) were washed twice with PBS (pH 7.4), resuspended in 5% perchloric acid, vigorously vortexed, and then allowed to stand on ice for 1 h. After centrifugation, the supernatant was transferred into a new tube, and half of the volume of 2 N NaOH was added, followed by 4 µl of benzoyl chloride. They were mixed and allowed to stand at 30 °C for 40 min. Adding saturated NaCl stopped the benzoylation, and the mixture was extracted with 2 volumes of diethyl ether. After separation by centrifugation, the upper layer was transferred into a new tube and dried in a stream of nitrogen. For analysis of polyamines, a Waters liquid chromatograph (Waters 2690, Waters Co.) equipped with a photodiode array detector (Waters 996, Waters Co.) set at 225 nm was applied. A symmetry C18 column (3.9 x 150 mm, 5 µM, Waters Co.) was used for the separation; the injection volume was 20 µl, and the gradient program proceeded from 50–85% methanol. The flow rate was 0.9 ml/min, and spermine was used as an internal standard.

cAMP Determination—Intracellular cAMP concentrations were determined using a cAMP Enzyme Immunoassay Kit (Assay Design Inc.). Cells grown overnight in LB were washed with a minimal M9 medium and subcultured to an initial OD600 of 0.01 in a minimal M9 medium containing 0.4% glucose and cultivated for 24 h. Cells were then harvested, washed, and resuspended to equal cell densities (1 x 1010) in 0.9% NaCl. cAMP concentrations of cells boiled for 10 min at 100 °C were determined following the manufacturer's recommendation. The cAMP concentrations were calculated from the typical standard curves, and the detectable range was 0.2–19.8 pmol/ml.

{beta}-Galactosidase Activity Assay—At the end of the growth period, an aliquot of each culture was transferred to new tubes containing 30 µg/ml chloramphenicol (final concentrations) and prechilled on ice. {beta}-Galactosidase activity was measured by monitoring the hydrolysis of o-nitrophenyl-{beta}-D-galactopyranoside (ONPG) as described by Miller and expressed as Miller units (21). Results were confirmed by at least two or three independent experiments.

GAD Activity Assay—Cells grown for 24 h in a minimal M9 medium containing 0.4% glucose were harvested, washed with 0.9% NaCl, and resuspended to equal cell densities (5 x 1010 cells/ml) in the same solution. A GAD activity assay was carried out using GAD reagent with some modifications (23). The GAD reagent consisted of1gof L-glutamic acid, 0.05 g of bromcresol green (colorimetric indicator), 90 g of NaCl, and 3 ml of Triton X-100 per liter. An aliquot of cells (5 x 109 cells) was transferred to a test tube, and 1 ml of GAD reagent was added, mixed immediately, and allowed to vortex vigorously for 30 s. The tubes were then incubated in a 35 °C water bath, and the change in pH was measured after 1 h.

Western Blot Analysis—Strains were grown in a minimal M9 medium containing glucose. At the stationary phase, cells were collected by centrifugation and washed twice with phosphate-buffered saline (pH 7.4). Protein extract was separated on to a 12.5% polyacrylamide gel. After transferring the proteins onto nitrocellulose membranes, the proteins were revealed using primary antibody (rabbit anti-H-NS, kindly supplied by Dr. E. Bremer; see Ref. 10), secondary antibodies (anti-rabbit antibody), and an ECL detection kit (Amersham Biosciences). Protein concentrations were determined by the Bradford method (4).

Measurement of Acid Survival Rate—Strains were grown in a minimal M9 medium containing glucose with and without polyamines (1 mM) until they entered the stationary phase. After 24 h of cultivation, the cells were harvested and resuspended in a PBS buffer (pH 7.0). Two aliquots of cells (each 1 x 108 cells per ml) were transferred to new tubes and adjusted to a 1-ml final volume using PBS buffer (pH 2.5 and 7.0, respectively). The mixtures were incubated for 1 h in a 37 °C water bath. Cells were serially diluted with PBS (pH 7.0), and viable cells were counted on a LB agar plate.


    RESULTS
 TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
Deficiency of GAD Activity Caused by the Genetic Inactivation of Both gadA and gadB Genes Causes Elevated Sensitivity under Low pH—Despite the importance of GAD under acidic conditions, no gadA/gadB double mutant phenotypes have been reported to date. We constructed a gadA/gadB double mutant in the polyamine-synthesizing wild-type background to investigate the role of GAD on normal cell growth. While each single mutant showed a normal growth pattern, the gadA/gadB double mutant showed a decreased growth rate (~70% of the level of the wild type) in a medium containing glucose as the carbon source (Fig. 1A).



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FIG. 1.
GAD activity and acid resistance. A, wild type (QC2461, •), single gadA (JIL581, {circ}), or gadB mutant (JIL580, {blacktriangledown}), and gadA/gadB double mutant (JIL685,{triangledown}) were grown in a minimal M9 medium containing glucose as the sole carbon source, and OD at 600 nm was measured at the times indicated. B, GAD activity of the stationary phase cells (wild-type QC2461 and its gadA and/or gadB mutant). Data from three independent experiments are expressed as mean ± S.D. C, visualization of GAD activity. Relative GAD activities of wild-type QC2461 and its gadA and/or gadB mutant in B were visualized by photographs of test tubes containing reaction mixtures and cells. D, acid resistance of the wild-type QC2461 and its gadA and/or gadB mutant. Data from two independent experiments are expressed as mean ± S.D.

 

We also assayed GAD activity of the wild type and gadA and/or gadB mutant by investigating the pH variation. Each single mutant containing either gadA or gadB mutation represented approximately half the activity of total GAD, and the gadA/gadB double mutant was completely devoid of GAD activity, indicating that GadA and GadB comprise the whole GAD activity (Fig. 1, B and C). When decarboxylation occurs, the pH of the GAD reagent progressively increases, causing the indicator color to change from yellow to green (23). The wild type containing normal gadA and gadB genes showed a green color with time, but the gadA/gadB double mutant sustained its yellow color. As might be expected, the single mutant in either gadA or gadB gene, showed mixed colors of green and yellow.

It is well known that GAD is needed for survival under low pH (6, 14). We compared acid resistance under an extreme acidic environment (pH 2.5) in the wild-type and gadA and/or gadB mutants. The wild type showed higher levels of acid resistance than the gadA or gadB single mutant and the gadA/gadB double mutant. Complete deficiency of GAD activity in the gadA/gadB double mutant showed a great sensitivity to low pH (Fig. 1D).

Polyamines Are Necessary to Keep Normal GAD Activity and Acid Resistance through the Induction of gadA and gadB Expression under Normal Aerobic Conditions—To investigate the expression patterns of gadA and gadB with growth time in the presence or absence of polyamines, we measured the {beta}-galactosidase activities of both genes under normal aerobic conditions in a minimal M9 medium containing glucose as the carbon source. We identified that a polyamine-deficient mutant showed growth reduction if polyamines were not provided (Fig. 2A). While the gadA expression was not significantly induced in the absence of polyamines, exogenous supplementation of polyamines restored gadA expression to the wild-type level (Fig. 2B). As well as gadA, the gadB expression was induced at an extremely low level in the absence of polyamines and greatly induced by the supplementation of polyamines (Fig. 2C). The above results indicate that polyamines are definitely required for the induction of both genes (gadA and gadB) in a minimal M9 medium containing glucose as the sole carbon source, even under normal aerobic conditions. gadA and gadB expression has been induced at the stationary phase in previous studies (8), and we confirmed those results.



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FIG. 2.
Regulation of gadA or gadB expression, modulation of GAD activity, and restoration of the deleterious effect of acid shock by polyamines. A, growth profiles of the wild type (QC2461, •) and the polyamine-deficient mutant (JIL601) in the absence ({circ}) or presence of polyamines ({blacktriangledown}, putrescine; {triangledown}, spermidine). Polyamines were added to each culture medium at 1 mM final concentrations when needed. B, gadA::lacZ expression of the wild type (JIL571, •) and the polyamine-deficient mutant (JIL650) in the absence ({circ}) or presence of putrescine ({blacktriangledown}) or spermidine ({triangledown}). C, gadB::lacZ expression. Symbols are identical to B. D, measurement of GAD activity in the wild type (wt, QC2461) and the mutant (JIL601). Cells were grown as described in the legend to Fig. 1B. Data from three independent experiments are expressed as mean ± S.D. PUT, putrescine; SPD, spermidine. E, relative GAD activity was visualized by taking photographs of the test tubes containing the reaction mixtures of D. F, acid survivability of the wild type (wt, QC2461) and the polyamine-deficient mutant (JIL601) at pH 2.5. Data from two independent experiments are expressed as mean ± S.D.

 

Based upon the above results, we directly measured GAD activities of the wild-type and the polyamine-deficient mutant type in the presence or absence of polyamines. While GAD activity was normal in the wild type, the polyamine-deficient mutant failed to show normal activity (Fig. 2D). GAD activity in the mutant was dramatically increased by exogenous supplementation of putrescine or spermidine. The polyamine-synthesizing wild type showed a green color with time, but the polyamine-deficient mutant grown in the absence of polyamines sustained its yellow color. The color changed dramatically from yellow to green with the addition of polyamines to the polyamine-deficient mutant strain, indicating that polyamines are essential for the maintenance of GAD activity (Fig. 2E).

Because a polyamine-deficient mutant is devoid of GAD activity as described above, we anticipated that a low environmental pH could lead to a severe deleterious effect on the polyamine-deficient mutant. Based upon the induction pattern of gad expression under normal aerobic conditions, we prepared stationary phase cells of the polyamine-deficient mutant and its isogenic polyamine-synthesizing wild type. Compared with the wild type, the mutant is at least 4,000 times more susceptible to an environmental pH of 2.5. However, exogenous supplementation of polyamines dramatically relieved the increased cytotoxicity by the acid-induced stress in the mutant (Fig. 2F).

Regulation of gad Expression by Polyamine/cAMP System—A regulatory mechanism via the action of the cAMP receptor protein (CRP) has been suggested, in which CRP acts as a negative regulator for gad expression (5). We tried to suggest a characteristic polyamine-induced regulatory circuit in acid resistance, investigating the role of cAMP in the induction of gad expression and the modulation of the cAMP level by polyamines. A cya mutant showed increased gadA and gadB expression, indicating that cAMP acts as a negative regulator for gad induction (Fig. 3A). GAD activity in the cya mutant was also visualized by applying a GAD reagent compared with its isogenic wild type (Fig. 3B). The cya mutant showed a dark green color, suggesting that the cya mutant contains higher GAD activity.



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FIG. 3.
cAMP effect on acid resistance. A, cAMP effect on the expression of gadA or gadB genes. {beta}-Galactosidase activities were measured from the wild type (wt, JIL733, and JIL734, containing either gadA::lacZ or gadB::lacZ fusion) and the cya mutant (JIL790 and JIL791, containing either gadA::lacZ or gadB::lacZ fusion). B, visualization of relative GAD activity of the wild-type QC2461 and its cya mutant JIL582. C, modulation of the intracellular cAMP level by polyamines in the wild type (wt, QC2461) and the polyamine-deficient mutant (JIL601). JIL582 (QC2461, cya) and JIL612 (JIL601, cya) were used as controls. Data from two independent experiments are presented as pmol/mg of protein and shown as mean ± S.D. D, effects of the cya mutant allele on gadA or gadB gene expression and acid resistance. Left panel, {beta}-galactosidase activities of the polyamine-deficient mutants JIL650 and JIL669 (cya+) containing either gadA or gadB::lacZ fusion, and their cya mutants (cya) JIL790 and JIL791 containing either gadA or gadB::lacZ fusion; Right panel, acid resistance of the polyamine-deficient mutant JIL601 and its cya mutant JIL612.

 

To reveal the relationship between cAMP and polyamines in acid resistance, we measured the intracellular cAMP level in the presence or absence of polyamines. Interestingly, the polyamine-deficient mutant strain contains a 4 times higher level of cAMP than the polyamine-synthesizing wild type, and polyamine supplementation to the polyamine-deficient mutant also decreased the intracellular cAMP concentration to the wild-type level (Fig. 3C). To investigate whether the polyamines cause the reduction of the intracellular cAMP level and this decreased cAMP induces gad induction, we examined gadA and gadB expression in a cya mutant background (Fig. 3D, left panel). The introduction of a cya mutation allele in a polyamine-deficient mutant elevated gad expression even if polyamines were not exogenously provided. The cya mutation also recovered its cytotoxicity shown in the polyamine-deficient mutant under pH 2.5 (Fig. 3D, right panel). These results strongly suggest that the polyamine signal enabling normal induction of gad expression and acid resistance resulted from the reduction of the intracellular cAMP level, acting as a negative regulator for gad induction and acid resistance.

Independent Regulation of gad Expression by H-NS/RpoS and Polyamine/cAMP Systems—Previous studies have suggested that two regulatory proteins, H-NS and RpoS, are involved in the regulation of gadA and gadB transcription. H-NS negatively regulates the expression of gad genes in the exponential phase, while alternative sigma factor RpoS positively regulates them in the stationary phase (5, 6, 8). Because gadA and gadB expression are induced in the stationary phase, we investigated their regulation by H-NS and RpoS in the stationary phase. Genetic inactivation of hns led to absolute repression of both gadA and gadB expression, indicating that H-NS acts as a positive regulator for gad induction in the stationary phase, unlike the previous results showing that H-NS acts as a negative regulator in the exponential growth phase (Fig. 4A). RpoS, another known regulator, was partially involved in gadA or gadB expression. An rpoS mutant also showed decreased gad expression, indicating that RpoS also acts as a positive regulator (See also Fig. 4A).



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FIG. 4.
Effect of H-NS or RpoS on gad gene expression. A, gad gene expression in the wild types (wt, JIL571 and JIL572) and their rpoS (JIL655 and JIL656) or hns (JIL657 and JIL658) mutant. Data from three independent experiments are presented as Miller units and shown as mean ± S.D. B, regulation of rpoS gene expression by H-NS. {beta}-Galactosidase activities were measured from hns+ (JIL666) and hns strain (JIL682). C, gadA expression by polyamine supplements in an rpoS or hns mutant. Levels of gadA gene expression of the polyamine-deficient (JIL650) mutant and its rpoS (JIL678) or hns (JIL679) mutant were measured in the absence (filled bar) or presence of polyamines (putrescine, gray hatched bar; spermidine, dark hatched bar). Data from three independent experiments are presented as Miller units and shown as mean ± S.D. D, gadB expression by polyamine supplements in an rpoS or hns mutant. Levels of gadB gene expression of the polyamine-deficient (JIL669) mutant and its rpoS (JIL680) or hns (JIL681) mutant were measured. Symbols are identical to C. Data from three independent experiments are presented as Miller units and shown as mean ± S.D. E, Western blot analysis of wild-type QC2461 and the polyamine-deficient mutant JIL601 in the presence or absence of polyamines. F, rpoS gene expression by polyamines. Polyamine-deficient mutant, JIL666, was grown in a minimal M9 medium in the absence (•) or presence of putrescine ({circ}) or spermidine ({blacktriangledown}). Data are presented as Miller units.

 

While RpoS partially regulates the expression of gad genes, H-NS absolutely regulates them, indicating that H-NS is a more predominant regulator than RpoS. Based on the major role of H-NS, we asked if H-NS could regulate rpoS. We constructed a strain containing a single rpoS::lacZ fusion in its chromosome, and measured its expression in an hns+ or hns background. Interestingly, rpoS was positively regulated by H-NS in the polyamine-deficient mutant (Fig. 4B). These results correspond to the result that H-NS is a main regulator, and RpoS is a less effective one in the regulation of gadA and gadB expression.

We also measured the expression level of gadA or gadB in the presence of polyamines in the mutants in order to ask whether polyamines could still induce gad expression in an hns or rpoS mutant background. Polyamines could no longer induce gad expression in the mutant, suggesting that normal H-NS or RpoS is necessary for the induction of gadA and gadB expression by polyamines (Fig. 4, C and D).

Because both polyamines and H-NS positively regulate gad expression, we examined their relationship in the regulation of gad expression. However, polyamines did not either regulate intracellular H-NS levels or regulate rpoS expression under this culture condition (Fig. 4, E and F). We also investigated the changes in the intracellular polyamine level in the hns+ (QC2461) and hns (JIL692) mutant background. Intracellular concentrations of putrescine and spermidine were same in both strains, indicating that H-NS did not alter the intracellular polyamine level (Table III). These results suggest that polyamines and H-NS independently participate in gad regulation.


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TABLE III
Intracellular polyamine concentration in the hns+ or hns- background

 


    DISCUSSION
 TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
Acid resistance is considered to be an important virulence factor of pathogenic E. coli strains such as O157:H7 (6). It is well known that GAD is needed for survival under low pH (6, 14). Cells possess specific defense mechanisms against acid environments in which the GAD system has been extensively studied because of its major role in the detoxification of acid-induced stress in E. coli. H-NS and RpoS are known to be common regulators that are associated with gad induction. This model proposes that H-NS acts as a negative regulator of gad induction in the logarithmic phase, and RpoS acts as a positive regulator of it in the stationary phase. In this study, we present a new model containing polyamines and cAMP as signaling molecules necessary for normal operation of the GAD system against acid-induced stress. In addition, we compare two distinct regulatory mechanisms, H-NS/RpoS and polyamine/cAMP systems, for the induction of gad expression, and show that polyamine/cAMP system has a major role in the defense mechanism against acid-induced stress as well as the H-NS/RpoS system.

There are many investigations demonstrating the fact that polyamines participate in various cellular processes including gene expression and protein biosynthesis, but the role of polyamines in an acid resistance has not been clarified (16). In this report, we suggested a specific defense mechanism that regulates gad expression via the positive function of polyamines. In addition, we reported that the two defense mechanisms, H-NS/RpoS and polyamine/cAMP system, comprise distinct regulatory circuits against acid environment. Finally, we suggested a possible model encompassing cell signaling.

Maintenance of GAD Activity by Polyamines Is Essential for Acid Resistance—The GAD system contains three genes. Two of the genes, gadA and gadB, each at separate locations, encode highly homologous GAD isoforms (24). A third gene, gadC, is functionally different from gadA and gadB and encodes a putative glutamate:GABA antiporter (6). GAD, the gene product of gadA and gadB, is a pyridoxal 5'-phosphate-dependent enzyme, catalyzing the irreversible conversion of glutamate and H+ to GABA and CO2. Following decarboxylation, one intracellular proton is consumed, and GABA is exported using the putative glutamate/GABA antiporter encoded by the gadC gene, which is located downstream of the gadB gene in the gadBC operon (8). As the result of sequential reactions, protons are consumed during acid stress and excreted from the cell, thereby preventing the internal pH from decreasing to lethal levels.

Although GAD has major roles in the defense mechanism against acid-induced damage, no studies have been made of the phenotype of the gadA/gadB double mutant. Concerned about the importance of GAD, we constructed a gadA/gadB double mutant to investigate its phenotype. GadA and GadB are similarly involved in a large range of GAD activities, with each contributing equally. The gadA/gadB double mutant was absolutely devoid of GAD activity. Reduced growth rate by the gadA/gadB double mutant in a minimal M9 medium containing glucose may be related to acid sensitivity. This double mutant showed severe susceptibility to acid stress, suggesting that GAD is necessary for acid resistance.

Our experiments indicated that a polyamine-deficient mutant was completely devoid of GAD activity, which was also true of the gadA/gadB double mutant. This complete deficiency of GAD activity resulted from the repression of gadA and gadB expression caused by polyamine deficiency, because exogenous supplementation of polyamines to the mutant enabled the cell to maintain normal GAD activity through up-regulation of the gad genes. Therefore, in the case of the gadA/gadB double mutant, the polyamine-deficient mutant showed increased susceptibility against acid-induced stress. On the other hand, exogenous supplementation of polyamines dramatically restored vulnerability to acid stress to wild-type levels. This strongly suggests that the cytotoxic effect by intracellular polyamine depletion in an acidic environment must be the result of the deficient GAD activity and that polyamines participate in specific defense mechanism that causes the recovery of normal GAD activity.

Polyamines Participate in the Detoxification of Acid-induced Stress by Reducing Intracellular cAMP Levels—cAMP is involved in the regulation of transcription, either positively or negatively, in many E. coli genes (3), and is also involved in the regulation of acid resistance (5, 6). It has been shown that the polyamine supplement to the E. coli B strain affected the hydrolyzing activity on cAMP by increasing the phosphodiesterase activity (33). This result suggested the possibility that polyamine could regulate intracellular cAMP levels. Based on the previous results, we hypothesized that the regulation of gad expression by polyamines during normal aerobic growth could be mediated by cAMP. To examine the possibility that the polyamine-mediated defense mechanism against acid-induced stress is mediated by cAMP, we measured the intracellular cAMP level in the polyamine-deficient mutant and its isogenic wild type. Interestingly, polyamines largely prevented cAMP accumulation. Furthermore, the introduction of a cya mutation allele into the polyamine-deficient mutant greatly induced both gadA and gadB expression, which led to increased acid resistance, even if polyamines were not provided. These results strongly suggest that the polyamine signal, acting as a positive regulator, enables effective gad induction, arising from the down-regulation of the intracellular cAMP level, acting as a negative regulator.

Positive Regulation of gad Gene Expression via H-NS/RpoS System—It has been suggested that H-NS protein directly or indirectly function as a transcriptional repressor (28, 30). Furthermore, H-NS has been implicated in post-transcriptional regulation of the RpoS stability (1, 30). Therefore, it could be hypothesized that nucleoid protein affected the expression of gad. Recent studies have also proposed that H-NS could directly bind gad promoters, thereby silencing transcription of gad genes (8). Those results have been obtained using cells growing exponentially in rich media. In this study, we suggest other roles for H-NS and RpoS in cells grown in the stationary phase using M9 minimal medium.

Stationary phase-grown cells containing hns mutation failed to induce gad expression. This evidence strongly suggests that there is another function of H-NS that positively regulates gad expression in the stationary phase, in addition to H-NS acting as a negative regulator for gad expression in the logarithmic growth phase. We also confirmed that the RpoS regulator positively regulates gad expression in the stationary phase. To identify signal transduction pathways involving H-NS and RpoS, we measured rpoS expression in both hns and hns+ backgrounds. Surprisingly, H-NS is definitely required for normal rpoS induction in the stationary phase, indicating that signal transduction from H-NS to gad is mediated by rpoS up-regulation via positive regulation of H-NS. We conclude that H-NS induces rpoS expression, thereby possibly enhancing the RpoS level, which may lead to gad induction. While H-NS is absolutely required for gad induction, RpoS is partially required for it. So, we conclude that H-NS regulates at least two different factors including RpoS. We do not know the other regulator affecting gad expression at this time, but RpoS must be the main regulator because its deficiency causes significant reduction of gad expression.

The Relation of Signal Transduction Pathways for the Induction of gadA and gadB by H-NS, RpoS, cAMP, and Polyamines—To investigate the relationship between H-NS/RpoS (positive regulation) and polyamine/cAMP (negative regulation) systems, we first measured the changes of intracellular H-NS level in the absence or presence of polyamines and the intracellular polyamine level in both hns and hns+ background. We could not demonstrate significant detectable changes in either the level of H-NS or the level of polyamines, indicating that H-NS and polyamines are not directly related to each other for gad expression. We confirmed that a polyamine-deficient mutant cell failed to induce gad expression irrespective of the existence of normal H-NS. However, normal level of H-NS was essential for the induction of gad expression by polyamines because the polyamine effect on gad up-regulation disappeared in the hns mutant background. Nonetheless, we concluded that although there were two regulatory systems in acid resistance, they seem distinct.

In conclusion, we showed that multiple signals including H-NS, RpoS, cAMP, and polyamines are involved in gad induction (Fig. 5). In these investigations, we examined the sequence of signal transduction or the relationship of the signals for normal gad induction. We demonstrated two distinct regulatory circuits. H-NS positively regulates rpoS gene expression and the rpoS gene product, RpoS, induces both gadA and gadB expression. cAMP negatively regulates both gadA and gadB induction, but the negative regulation is suppressed by polyamines. The H-NS/RpoS and polyamine/cAMP systems operate independently to achieve acid resistance.



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FIG. 5.
Proposed model for signal pathway in acid resistance. (–), negative regulation; (+), positive regulation.

 


    FOOTNOTES
 
* This work was supported by the Nuclear Research and Development Program from the Ministry of Science and Technology (MOST) of the Republic of Korea. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Back

{ddagger} To whom correspondence should be addressed: Dept. of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Inst., P. O. Box 105, Yusong, Taejon 305-600, Korea. Tel.: 82-42-868-8031; Fax: 82-42-861-9560; E-mail: igkim{at}kaeri.re.kr.

1 The abbreviations used are: GAD, glutamate decarboxylase; CRP, cAMP receptor protein; GABA, {gamma}-aminobutyrate; H-NS, histone-like protein; PUT, putrescine; RpoS, RNA polymerase {sigma} subunit; SPD, spermidine; wt, wild type; PBS, phosphate-buffered saline. Back


    ACKNOWLEDGMENTS
 
We greatly thank Dr. H. Tabor, Dr. J. W. Foster, and Dr. D. Touati for providing E. coli strains and Dr. R. Hengge-Aronis for providing plasmid (pRL45). We also thank Dr. E. Bremer for the kind gift of H-NS antibody.



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