The Methanococcus jannaschii dCTP Deaminase Is a Bifunctional Deaminase and Diphosphatase*

Hong Li, Huimin Xu, David E. Graham, and Robert H. WhiteDagger

From the Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0308

Received for publication, December 6, 2002

    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Most bacteria produce the dUMP precursor for thymine nucleotide biosynthesis using two enzymes: a dCTP deaminase catalyzes the formation of dUTP and a dUTP diphosphatase catalyzes pyrophosphate release. Although these two hydrolytic enzymes appear to catalyze very different reactions, they are encoded by homologous genes. The hyperthermophilic archaeon Methanococcus jannaschii has two members of this gene family. One gene, at locus MJ1102, encodes a dUTP diphosphatase, which can scavenge deoxyuridine nucleotides that inhibit archaeal DNA polymerases. The second gene, at locus MJ0430, encodes a novel dCTP deaminase that releases dUMP, ammonia, and pyrophosphate. Therefore this enzyme can singly catalyze both steps in dUMP biosynthesis, precluding the formation of free, mutagenic dUTP. Besides differing from the previously characterized Salmonella typhimurium dCTP deaminase in its reaction products, this archaeal enzyme has a higher affinity for dCTP and its steady-state turnover is faster than the bacterial enzyme. Kinetic studies suggest: 1) the archaeal enzyme specifically recognizes dCTP; 2) dCTP deamination and dUTP diphosphatase activities occur independently at the same active site, and 3) both activities depend on Mg2+. The bifunctional activity of this M. jannaschii enzyme illustrates the evolution of a suprafamily of related enzymes that catalyze mechanistically distinct reactions.

    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Deoxyuridine nucleotides pose severe problems for cells. Although necessary precursors for thymine nucleotide biosynthesis, deoxyuridine nucleotides can interfere with DNA polymerase activity, either by inhibiting polymerases or by causing them to misincorporate 2'-deoxyuridine 5'-triphosphate (dUTP) in place of 2'-deoxythymidine 5'-triphosphate (dTTP) (1, 2). Ubiquitous DNA glycosylase enzymes remove uracil bases from DNA, which are otherwise formed by spontaneous cytosine deamination. The resulting apyrimidinic sites are cleaved by endonucleases, fragmenting cellular DNA (3).

At temperatures of 70-95 °C, rate constants for spontaneous cytosine deamination (in denatured DNA or cytidine nucleotides) are several orders of magnitude higher than those measured at more moderate temperatures (4). Therefore hyperthermophilic archaea that grow at such temperatures counter the mutagenic effects of cytosine deamination with a variety of thermostable uracil-DNA glycosylase enzymes (5). As a consequence of having efficient base excision repair mechanisms, these organisms must avoid misincorporating deoxyuridine nucleotides into DNA during replication by using highly discriminatory DNA polymerases and by maintaining low dUTP levels in the cell (1, 6).

Eukaryotes, some bacteria and some archaea use zinc-dependent cytidine or deoxycytidine deaminase enzymes to produce uridine or deoxyuridine nucleotides. Yet many bacteria, including Escherichia coli and Salmonella typhimurium, produce most of their 2'-deoxyuridine 5'-monophosphate (dUMP) from 2'-deoxycytidine 5'-triphosphate (dCTP) using two different enzymes. These bacteria use dCTP deaminase (DCD,1 EC 3.5.4.13) to catalyze the nucleophilic substitution of a hydroxide ion for the 4-amino group of the cytosine base (7). A second enzyme, dUTP diphosphatase (DUT, EC 3.6.1.23), catalyzes the hydrolysis of the dUTP alpha -phosphorus anhydride bond to produce pyrophosphate (diphosphate) and dUMP, the obligatory precursor for thymidine nucleotides synthesis (8) (Fig. 1). Due to the toxicity of dUTP produced by DCD, the DUT enzyme is required to remove the accumulated product. Null mutations in the E. coli dut gene are lethal unless suppressed by null mutations in the dcd gene (9).

Despite catalyzing markedly different hydrolytic reactions, DUT and DCD proteins are encoded by homologous genes (9, 10). dUTP diphosphatase enzymes have been extensively studied, and four crystal structure models have been published for DUT proteins from E. coli (11), human (12), and two retroviruses (13, 14). However, only one DCD enzyme from S. typhimurium has been partially purified and characterized (15) and the dcd gene was subsequently identified in E. coli (9).

DUT proteins share five conserved signature motifs with the DCD proteins; four of these correspond to conserved uridine binding regions in DUT. These conserved domains have been used to identify dUTP diphosphatase homologs from diverse organisms (16) and to predict that two paralogous genes in the hyperthermophilic archaeon Methanococcus jannaschii encode DCD and DUT proteins (10). The original annotation of the complete genome sequence from that organism described both gene products as dCTP deaminases because of their considerable sequence divergence from canonical DUT proteins (17).

In this work, we describe the characterization of two paralogous genes from M. jannaschii that are members of the dut-dcd family. Both genes were cloned in E. coli, and the heterologously expressed proteins were purified and characterized. The protein encoded by locus MJ1102 was shown to be a dUTP diphosphatase, similar to previously characterized DUT homologs. Unexpectedly, the enzyme encoded by locus MJ0430 was found to be a dCTP deaminase/diphosphatase (MjDCD-DUT). It has two functions, catalyzing both the deamination of dCTP and release of pyrophosphate to produce dUMP. It also acts as a dUTP diphosphatase with a lower affinity for dUTP than for dCTP. Compared with the S. typhimurium dCTP deaminase, MjDCD-DUT has a higher affinity for dCTP and is a more active deaminase. The discovery of this bifunctional enzyme ties together the apparently unrelated deaminase and diphosphatase activities of DCD and DUT homologs and shows how M. jannaschii circumvents the problem of accumulating dUTP during thymidine biosynthesis.

    EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Materials-- All reagents were purchased from Sigma unless otherwise specified. Stock solutions of nucleotides were prepared in deionized water. Nucleotide concentrations were determined in sodium phosphate buffer (pH 7.2, 0.1 M NaCl) by UV spectroscopy using established molar extinction coefficients for cytidine nucleotides, uridine nucleotides, adenosine nucleotides, and guanosine nucleotides (18).

Cloning and Expression of the MJ0430 and MJ1102 Proteins in E. coli-- The M. jannaschii genes at loci MJ0430 (Swiss-Prot accession number Q57872) and MJ1102 (Swiss-Prot accession number Q58520) were amplified by PCR from genomic DNA using oligonucleotide primers synthesized by Invitrogen. The MJ0430 gene was amplified using primers: Fwd, (5'-GGTGGTCATATGATTCTTAGTGATAAAG-3') and Rev, (5'-GATCGGATCCTTAATCTTTTTTATGATTGTC-3'). The MJ1102 gene was amplified using primers: Fwd, (5'-GGTGGTCATATGGTGGTGAAATTAATG-3') and Rev, (5'-GATCGGATCCTTATCTCCCTTTATAAATTC-3'). The forward primers introduced an NdeI restriction site at the 5'-end of the amplified DNA whereas reverse primers introduced a BamHI site at the 3'-end. PCR amplifications were performed as described previously using a 50 °C annealing temperature for MJ0430 and a 55 °C annealing temperature for MJ1102 (19). PCR products were purified using a QIAQuick spin column (Invitrogen) and then digested with NdeI and BamHI restriction enzymes (Invitrogen). Bacteriophage T4 DNA ligase (Invitrogen) was used to ligate the MJ0430 and MJ1102 genes into compatible sites in plasmids pET17b (Novagen) or pT7-7, respectively (20). DNA sequences were verified by dye-terminator sequencing at the University of Iowa DNA facility. The resulting recombinant plasmids were transformed into E. coli BL21-CodonPlus(DE3)-RIL (Stratagene) cells.

Transformed E. coli cells were grown in Luria-Bertani medium (200 ml; Difco) supplemented with 100 µg/ml ampicillin. Cultures were shaken at 37 °C until they reached an absorbance at 600 nm of 0.8. Recombinant protein production was induced with 28 mM lactose. After an additional 2-h incubation with shaking at 37 °C, the cells were harvested by centrifugation (4000 × g, 5 min) and frozen at -20 °C. Induction of the desired proteins was confirmed by SDS-PAGE analysis of total cellular proteins.

Site-directed Mutagenesis-- To test the possible function of conserved Asp135 and Glu145 residues of MjDCD-DUT, Asp135 was replaced by Asn (D135N) and Glu145 was replaced by Gln (E145Q). The QuikChangeTM site-directed mutagenesis kit (Stratagene) was used according to the manufacturer's instructions with template pMJ0430 (MJ0430 in pET17b). The oligonucleotide primers (Invitrogen) used were D135N-Fwd (5'-CTGCTGGATGGATTAACGCTGGATTTAAAGG-3'), D135N-Rev (5'-CCTTTAAATCCAGCGTTAATCCATCATCCAGCAG-3'), E145Q-Fwd (5'-GGAAAAATAACCTTGCAGATTGTTGCTTTCG-3'), and E145Q-Rev (5'-CGAAAGCAACAATCTGCAAGGTTATTTTTCC-3'). DNA sequences of the mutated genes were confirmed by dye-terminator sequencing at the University of Iowa DNA facility.

Purification of MjDCD-DUT-- Heterologously expressed MjDCD-DUT protein was purified from soluble cell-free extract by heat treatment and chromatographic methods. E. coli cells (13 g, wet weight) were suspended in 25 ml of extraction buffer (20 mM Tris/HCl, 2 mM DL-dithiothreitol (DTT), pH 7.6) and sonicated in a 50-ml plastic tube using a microprobe tip driven by a W-385 Ultrasonic processor (Heat Systems-Ultrasonics, Inc.) operating at 50% power output. Samples cooled by ice water were sonicated for 8 min with 3 s on/off intervals. Soluble cell-free extract was obtained after centrifugation at 20,000 × g for 15 min at 4 °C. The E. coli proteins were denatured by heating the soluble cell-free extract at 70 °C for 15 min, followed by centrifugation at 20,000 × g for 10 min at 4 °C to remove the insoluble material. Heat-soluble cell extract (16 ml) was applied to a Mono Q HR anion-exchange column (1 × 8 cm; Amersham Biosciences) equilibrated with buffer A (20 mM Tris/HCl, pH 7.5). Pumps attached to the column were controlled by a Biologic HR workstation (Bio-Rad). Bound protein was eluted with a 45-ml linear gradient from 0 to 1 M NaCl in buffer A at a flow rate of 1.0 ml/min. Fractions containing dCTP deaminase activity were pooled and concentrated in a N2-pressurized stirred cell with a YM10 ultrafiltration membrane (Millipore). Concentrated protein was applied to a Sephacryl S-200 HR size exclusion column (1.6 × 60 cm; Amersham Biosciences) equilibrated with buffer B (50 mM HEPES/NaOH, 0.15 M NaCl, 2 mM DTT, pH 7.2). MjDCD-DUT protein was eluted at a flow rate of 0.5 ml/min and was collected in 1-ml fractions. Fractions containing dCTP deaminase activity were pooled and concentrated in a N2-pressurized stirred cell with a YM10 ultrafiltration membrane (Millipore) and then stored at -70 °C. Heterologously expressed MJ1102-encoded protein and the MjDCD-DUT D135N and MjDCD-DUT E145Q variant proteins were purified by the same procedure.

Protein purity was evaluated by silver diamine staining of proteins separated by SDS-polyacrylamide gel electrophoresis (12% T, 4% C acrylamide) using a Tris/glycine buffer system. Sizes of the denatured proteins were determined by comparison to low molecular weight protein standards (Bio-Rad). Protein concentrations were measured using the Bradford total protein assay (Bio-Rad) with bovine serum albumin as the standard.

Analytical Size Exclusion Chromatography-- Size exclusion chromatography was performed at room temperature on a Superose 12HR column (1 × 30 cm; Amersham Biosciences). The column was equilibrated in buffer B and run with a flow rate of 0.4 ml/min. Protein standards used to calibrate the sizing column were horse spleen apoferritin (440,000), potato beta -amylase (200,000), yeast alcohol dehydrogenase (150,000), bovine serum albumin (67,000), bovine erythrocyte carbonic anhydrase (29,000), and horse heart cytochrome c (12,400). Eluted protein was detected by their absorbance at 280 nm and/or dCTP deaminase activity.

MALDI-TOF Mass Spectrometry of MjDCD-DUT Protein-- Purified MjDCD-DUT protein was analyzed by MALDI-TOF mass spectrometry. A saturated solution of 3,5-dimethoxy-4-hydroxycinnamic acid matrix was prepared in 50% (v/v) acetonitrile with 0.1% trifluoroacetic acid. Purified MjDCD-DUT (0.5 µl of a 2.2 mg/ml protein solution) was mixed with an equal volume of matrix solution on a stainless steel target (Kratos Analytical). Dried, crystalline samples were washed with 5 µl of 0.1% trifluoroacetic acid and dried under a stream of N2 gas. Samples were analyzed in a Kratos KOMPACT SEQ MALDI-TOF instrument (Kratos Analytical) operated on the linear mode at 20 kV. For each sample, 50 spectra were collected by scanning across the sample. Average masses were identified using the combined sample set. Ions of sodium, matrix, bovine serum albumin, bovine pancreas insulin chain B (oxidized), and horse heart cytochrome c were used for mass calibration.

Measurement of dCTP Deaminase Activity-- dCTP deaminase activity was measured using a continuous spectrophotometric assay (15). In a thermostated quartz cuvette, the following reaction mixture (1 ml) was equilibrated at 60 °C for 15 min: 50 mM TES/NaOH buffer (pH 7.5), 0.1 M NaCl, 5 mM MgCl2, 1 mM 2-mecaptoethanol (Fisher), and enzyme (0.1-1 µg). The reaction was initiated by addition of dCTP to a final concentration of 0.2 mM. The decrease in absorbance as a result of deamination of cytosine compounds was measured at 284 nm with an UV-VIS recording spectrophotometer UV-160A (Shimadzu). The molar extinction coefficient difference between deoxycytidine and deoxyuridine compounds is 3.8 × 103 M-1 cm-1 (15).

Measurement of dUTP Diphosphatase Activity-- Standard dUTP diphosphatase activity assays included 50 mM TES/NaOH buffer (pH 7.5), 0.1 M NaCl, 5 mM MgCl2, 1 mM 2-mecaptoethanol, and enzyme in 200 µl. The reaction mixture was pre-incubated at 60 °C for 15 min before the addition of dUTP. After 10 min of incubation, reaction was terminated by the addition of 10 µl of 100 mM pH 8.0 EDTA and cooled on ice. Pyrophosphate released from dUTP was measured using an enzymatic assay in a pyrophosphate reagent kit (Sigma). Alternatively, inorganic phosphate was produced from pyrophosphate by including a thermostable coupling enzyme in the dUTP diphosphatase assay. The coupling enzyme used was purified, heterologously expressed GTP cyclohydrolase from M. jannaschii, which has pyrophosphate phosphohydrolase activity, but has no effect on pyrimidine nucleotides (21). Inorganic phosphate was then quantified by the malachite green dye-enhanced phosphomolybdate assay (21). Inorganic phosphate concentrations were calculated from a standard curve using 0-8 nmol of KH2PO4 in 700 µl deionized H2O. Absorbances from reactions incubated without enzyme were subtracted from absorbances of the enzymatic reactions to calculate activities.

Product Identification-- The nucleotide products of the enzymatic reaction were analyzed by separation on a Mono Q HR anion-exchange column (0.5 × 5 cm, Amersham Biosciences). Bound nucleotides were eluted with a 25-ml linear gradient from 0 to 0.5 M NaCl in a 25 mM Tris/HCl buffer (pH 8.5) at a flow rate of 0.5 ml/min. The nucleotides eluted were monitored at 254 nm. Nucleotide standards were analyzed on the column before and after the sample runs for calibration. The nucleotides (and their retention times) were dCMP (20.1 min), dUMP (21.5 min), dCDP (24.1 min), dUDP (25.3 min), dCTP (26.9 min), and dUTP (27.9 min).

Measurement of the Regiospecificity of the O-P Bond Cleavage Reaction-- To 35 µl of buffer containing 50 mM TES/NaOH (pH 7.5), 5 mM MgCl2, and 1 mM mercaptoethanol were added 45 µl of H218O (98 atom% 18O) and 5 µl of a 2.2 mg/ml solution of the MjDCD-DUT protein. The sample was incubated at 60 °C for 15 min before 10 µl of 0.1 M dCTP was added. After an additional 15 min at 60 °C the sample was evaporated to dryness with a stream of dry nitrogen gas, and the residue was reacted at 100 °C with 20 µl of a TMS reagent composed of trimethylchlorosilane, hexadimethyldisilazane, and pyridine (1:3:9, v/v/v). After removal of the insoluble material by centrifugation, electron impact-mass spectrometry (EI-MS) analysis of the soluble material was performed using a VG-70-70EHF instrument (VG Analytical) operating at 70 eV with a direct probe inlet (probe temperature programmed from room temperature to 400 °C at 5 °C/s). The mass range scanned was 50-750 m/z at 5 s/decade.

Heat Stability, pH Optimum, and Metal Effect on dCTP Deaminase Activity of MjDCD-DUT-- To test the heat stability, purified MjDCD-DUT was incubated for 10 min at temperatures from 60 to 100 °C and then assayed for dCTP deaminase activity using the standard assay at 60 °C. The effect of pH on dCTP deaminase activity of MjDCD-DUT was studied using a three-component buffer system in the pH range 5.0-9.8 (22). Buffer mixture containing 75 mM bis-Tris, 38 mM HEPPS, and 38 mM CHES were adjusted to pH 5.5-9.5 using either NaOH or HCl. Effects of NaCl (Fisher), DTT, and 2-mecaptoethanol (Fisher) were tested in standard dCTP deamination assays.

Divalent cation dependence was examined using enzyme, TES buffer, and dCTP freed of divalent cations by passage through a 7.5 mm × 5 cm column of Chelex 100-Na+ (Bio-Rad) (21, 23). MjDCD-DUT elution was monitored by its absorbance at 280 nm. Metal replacement reactions included 5 mM concentrations of MgCl2·6H2O (Fisher), MnCl2·4H2O (Fisher), CaCl2·6H2O (Fisher), NiCl2·6H2O, CoCl2·6H2O (J. T. Baker Inc.), CuCl2·6H2O (J. T. Baker Inc.), BaCl2·2H2O (Fisher), or ZnSO4·7H2O.

Substrate Specificity and Inhibitory Effects of Other Nucleotides on MjDCD-DUT Deaminase Activity-- The substrate specificity of MjDCD-DUT was investigated using a spectroscopic assay. Nucleotide substrates tested at 0.2 and 1 mM concentrations included dCDP, dCMP, CTP, CDP, CMP, cytosine, deoxycytidine, ATP, dTTP, UTP, and GTP. The products of each reaction were identified by the Mono Q HR anion-exchange column (0.5 × 5 cm; Amersham Biosciences) as described above.

Inhibitory effects of various nucleotides on MjDCD-DUT dCTP deaminase activity were also examined. Enzyme activity was measured by quantifying the production of pyrophosphate. These reactions included 0.2 mM dCTP and 1 mM concentrations of other nucleotides.

Kinetic Analysis of MjDCD-DUT Activities-- For both dCTP deaminase and dUTP diphosphatase activities of MjDCD-DUT, initial reaction rates were measured in standard assays at various concentrations of dCTP (0-100 µM) or dUTP (0-800 µM). Reaction mixtures were incubated at 60 °C for 15 min, and then reactions were started by the addition of substrate. A curve from the Michaelis-Menten equation was fit through the initial rate data by nonlinear regression, using the Sigma Plot 2000 program (SPSS Science).

    RESULTS
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Identification, Expression, and Purification of MjDCD-DUT-- dCTP deaminase/dUTP diphosphatase homologs have been identified in the genomes of several archaea (10). Two homologs, MJ0430 and MJ1102, were found in the complete genome sequences of M. jannaschii (17). However, it was not known whether one gene product functions as a dUTP diphosphatase and the other as a dCTP deaminase. We cloned both genes and expressed both proteins in E. coli for purification and characterization.

The recombinant MjDCD-DUT (encoded by MJ0430) was purified through heating, anion-exchange, and gel-filtration chromatography. Table I shows the purification of 33 mg of MjDCD-DUT from 13 g of E. coli cells. The specific activity of purified MjDCD-DUT was 10.5 µmol/min/mg. Enzyme specific activity increased 4.2-fold though the purification, which indicates the MjDCD-DUT constitutes 24% of the total E. coli soluble protein.


                              
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Table I
Purification of M. jannaschii MjDCD-DUT

Analysis of the purified MjDCD-DUT by MALDI-TOF mass spectrometry showed a molecular mass of 23,619 ± 94 Da, which is close to its predicted 23,432 Da molecular mass from the genomic data. The denatured protein migrated on SDS-polyacrylamide gel an apparent molecular mass of 30,800 Da. From a Sepharose 12 HR size exclusion column, MjDCD-DUT eluted with an apparent molecular mass of 136,000 Da. This elution pattern suggests that native MjDCD-DUT protein may form a hexamer.

MjDCD-DUT Catalytic Activities and Reaction Products-- When MjDCD-DUT was incubated with 0.2 mM dCTP at 60 °C, the enzyme not only catalyzed the deamination of dCTP, but also catalyzed the release of pyrophosphate to form dUMP. MjDCD-DUT can also catalyze the hydrolysis of dUTP to form dUMP and pyrophosphate. These results show that MjDCD-DUT is bifunctional: it catalyzes two consecutive reactions to form dUMP using dCTP as substrate. In contrast, gamma -proteobacteria require separate dCTP deaminase and dUTP diphosphatase enzymes to catalyze the reactions (Fig. 1) (15).


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Fig. 1.   Reactions catalyzed by dCTP deaminase, dUTP diphosphatase, and MjDCD-DUT.

Enzymes can catalyze pyrophosphate release by promoting the nucleophilic substitution by water at either the alpha - or beta -phosphorus atoms of nucleotide triphosphates (24). To test the regiospecificity of MjDCD-DUT, the enzyme was incubated with dCTP in H218O, and the reaction products were converted to TMS derivatives. EI-MS analysis identified two compounds. The first corresponded to the (TMS)4 derivative of pyrophosphate, M+ = 466 m/z and M+-15 = 451 m/z. Isotopic clusters of these ions had the same relative intensities observed for unlabeled pyrophosphate and showed no 18O incorporation. The second identified compound consisted of the (TMS)4 derivative of dUMP M+-15 = 509 m/z. The isotopic cluster of this ion showed the incorporation of two labeled oxygens each with ~50% 18O enrichment. A fragment ion 299 m/z originating from the phosphate of the dUMP derivative contained only a single 18O confirming that one of the oxygens resided on the phosphate. The other oxygen was incorporated at the C-4 position of uracil during deamination. These data suggest that the phosphate anhydride cleavage proceeds by attack of activated water at the alpha -phosphate of 5'-nucleotide triphosphates.

Characterization of MjDCD-DUT Activity-- The standard dCTP deaminase assay was used to characterize the purified MjDCD-DUT under various reaction conditions. When incubated with 0.1 M NaCl, the enzyme exhibited its maximum activity, compared with no NaCl (70%), 0.05 M (70%), 0.15 M (90%), or 0.2 M NaCl (90%) added. No change of enzyme activity was observed when MjDCD-DUT was assayed in 50 mM TES/NaOH (pH 7.5), 50 mM HEPES/NaOH (pH 7.5), or 50 mM MOPS/NaOH (pH 7.5) buffer. Nor did addition of 1 mM 2-mercaptoethanol and 1 mM DTT affect the activity, although 1 mM 2-mercaptoethanol was added in the standard assay.

Purified MjDCD-DUT is a thermostable enzyme. When heated at 90 °C for 10 min, MjDCD-DUT retained over 70% of its activity, assayed at 60 °C. Assays of MjDCD-DUT dCTP deaminase activity at various pH showed maximum activity at pH 7.5. As shown in Fig. 2, over the range from pH 6 to 9, the enzyme retains more than 50% of its maximum activity.


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Fig. 2.   MjDCD-DUT dCTP deaminase activity at different pH values. A three-component buffer system was used with bis-Tris, HEPPS and CHES as described under "Experimental Procedures." Maximum specific activity in this experiment was 10.5 µmol/min/mg.

The substrate specificity of MjDCD-DUT was investigated using the standard deamination assay with 1 mM concentrations of other cytidine nucleotides. dCDP is a very poor substrate; MjDCD-DUT catalyzes its deamination and hydrolysis to form dUMP at only 4% of the rate of dCTP deamination. None of the following nucleosides or nucleotides was deaminated by MjDCD-DUT: dCMP, CTP, CDP, CMP, cytosine, or deoxycytidine. Neither ATP, dTTP, UTP, or GTP were substrates for MjDCD-DUT. Nor was pyrophosphate released in these assays. These results indicate that MjDCD-DUT is highly specific for dCTP as its substrate for deamination. The MjDCD-DUT activity requires metals. Divalent cation requirement was examined by assaying metal-free MjDCD-DUT. No activity was detected in reactions without added metals. Maximum dCTP deamination activity was observed with the addition of 2-5 mM MgCl2 in the presence of 0.2 mM dCTP. No other divalent cations supported full activity at the concentration of 5 mM (Table II). Compared with its dCTP deaminase activity, dUTP diphosphatase activity of MjDCD-DUT showed a similar pattern of metal activation (Table II).


                              
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Table II
Effects of added divalent cations on MjDCD-DUT dCTP deamination and dUTP diphosphatase activity

To test the inhibitory effects of other naturally occurring nucleotides on MjDCD-DUT dCTP deaminase activity, 1 mM concentrations of dCDP, dCMP, CTP, CDP, CMP, ATP, GTP, UTP, or dTTP were added to standard assays that included 0.2 mM dCTP. Enzyme activity was analyzed by measuring the production of pyrophosphate using the Sigma pyrophosphate reagent. Among these nucleotides, only dTTP inhibited enzyme activity. The relative activity was 58% compared with reactions without added dTTP. Because dUTP is a substrate for MjDCD-DUT diphosphatase activity, the inhibitory effects of dUTP were tested using the deamination assay. Here, 1 mM dUTP inhibited the activity by 80%.

To characterize the kinetic properties of MjDCD-DUT, initial rates for both dCTP deaminase and dUTP diphosphatase activities were measured at various substrate concentrations. The relevant substrate for both reactions is likely a Mg2+-substrate complex. Therefore, in these experiments, the Mg2+ concentration was fixed at 5 mM, more than a 5-fold excess over the substrate concentrations. Both sets of activity data were fit to the Michaelis-Menten first-order rate equation. At 60 °C, apparent kinetic parameters of MjDCD-DUT were Km = 17.6 ± 2.6 µM, Vmax = 14.7 ± 0.87 µmol/min/mg for dCTP deamination, and Km = 263 ± 64 µM, Vmax = 23.9 ± 2.6 µmol/min/mg for dUTPase activity.

Because both dCTP and dUTP are substrates for MjDCD-DUT diphosphatase activity, a competition assay was used to test the relationship of these two activities (25). Substrate concentrations were chosen to give similar activities with 100% dCTP (40 µM) or 100% dUTP (150 µM). The proportion of each substrate in the reactions was varied from 100% dCTP and 0% dUTP to 0% dCTP and 100% dUTP. The combined activity for pyrophosphate production was quantified by measuring the phosphate produced when the reaction was coupled to a thermostable pyrophosphate phosphohydrolase enzyme (21). The results were consistent with a general model for reactions occurring at the same catalytic site and competing with each other, indicating that dCTP and dUTP bind at the same sites (data not shown) (25).

Sequence Analysis and Site-directed Mutation of MjDCD-DUT-- An alignment of the MjDCD-DUT protein sequence with homologous DCD and DUT sequences identified conserved amino acids in four motifs that were previously described for dUTP diphosphatases (Fig. 3) (16). Crystal structure models of bacterial and viral DUT proteins implicate one of these conserved residues, corresponding to Asp135 of motif 3 of MjDCD-DUT, in binding the 3'-OH group of the substrate's deoxyribose moiety (11-13). The bacterial enzyme may also use the corresponding residue to activate a water molecule for nucleophilic attack on the substrate's alpha -phosphate group (11). The viral DUT protein could use this aspartyl side chain to promote subunit interactions (14). To test this residue's importance to catalysis, we used site-directed mutagenesis to replace Asp135 with Asn (MjDCD-DUT D135N). Purified MjDCD-DUT D135N had neither dCTP deaminase nor dUTP diphosphatase activity. The altered protein had an apparent molecular mass of 57,900 Da, measured by analytical gel-filtration chromatography, compared with a mass of 136,000 Da for the wild-type MjDCD-DUT. Thus the replacement of Asp135 may destabilize the protein's quaternary structure.


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Fig. 3.   Sequence alignment of MjDCD-DUT (Swiss-Prot accession Q57872) with homologs from Aquifex aeolicus (AaDCD; Swiss-Prot accession O67539), Mycobacterium tuberculosis (MtDCD; Swiss-Prot accession O07247), E. coli (EcDCD and EcDUT; Swiss-Prot accession P28248 and P06968), Helicobacter pylori (HpDCD; Swiss-Prot accession O25136), M. jannaschii (MjDUT; Swiss-Prot accession Q58520), Pyrococcus furiosus (PfDUT; GenBankTM accession AAL47572.1), archaeal virus SIRV (SIRV-DUT; GenBankTM accession 666605.1), Homo sapiens (HsDUT; Swiss-Prot accession P33316; positions 112-252). Boxed residues indicate five previously described motifs shared by dUTP diphosphatases. Positions of identically conserved residues are shown in white on black and regions of similarity conserved residues are boxed. Two arrows indicate the Asp135 and Glu145 residues replaced by directed mutagenesis. DUT and DCD sequences were aligned separately using the ClustalW program (39), and then the alignments were combined by profile alignment using ClustalW.

In the analogous bacterial cytidine deaminase enzymes, the side chain of a conserved glutamate residue is proposed to act as a general acid/base catalyst, facilitating release of the leaving -NH2 group after a nucleophilic hydroxide ion attacks the cytosine base (26-28). Although MjDCD-DUT has no sequence similarity to this zinc-dependent cytidine deaminase, MjDCD-DUT homologs do share a conserved glutamate residue (Glu145). Because this glutamate residue is conserved in all DCD homologs, but in few DUT homologs, we used site-directed mutagenesis to replace Glu145 with Gln (MjDCD-DUT E145Q). Purified MjDCD-DUT E145Q protein had no dCTP deaminase activity, but retained 25% dUTP diphosphatase activity relative to wild-type enzyme. The purified MjDCD-DUT E145Q also has a higher apparent affinity for dUTP compared with the wild-type MjDCD-DUT (Table III). This residue is also conserved in the archaeal virus SIRV DUT enzyme (described below) that lacks dCTP deaminase activity, therefore these results suggest that the Glu145 residue is necessary, but not sufficient, for dCTP deaminase activity.


                              
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Table III
Comparison of apparent kinetic constants for dCTP deaminases and dUTP diphosphatases

Characterization of MJ1102-encoded Protein-- Purified MJ1102-encoded protein showed a single band on a SDS-polyacrylamide gel with an apparent molecular mass of 18,000 Da, compared with its predicted molecular mass of 18,640 Da. From the Sepharose 12 HR size exclusion column, the MJ1102-encoded protein eluted with an apparent molecular mass of 48,200 Da, which suggests that the native protein may form a trimer. When incubated at 60 °C, it catalyzed hydrolysis of dUTP to dUMP and pyrophosphate. It had no detectable dCTP deaminase activity. Therefore the MJ1102 gene encodes a dUTP diphosphatase.

Sequence Alignment of MjDUT-DCD Homologs-- Fig. 3 shows the amino acid sequences of MjDCD-DUT and MJ1102 proteins aligned with sequences from homologous DCD proteins and four previously characterized DUT enzymes. All of the sequences share conserved residues in five previously defined sequence motifs (10, 16). Crystal structure models of homotrimeric DUT proteins show each active site is comprised of residues from motifs 2, 3, 4, or 5 contributed by all three subunits. We predict that the hexameric MjDCD-DUT protein adopts a similar fold and active site geometry. Despite the previous identification of a set of residues specific to DCD but not DUT homologs (10), few of those amino acid positions are conserved in an alignment containing more diverse sequences (Fig. 3). Instead, residues in the carboxy-terminal region of the DCD homologs distinguish DCD from DUT proteins and may act alongside the Glu145 residue to confer deaminase activity on DCD enzymes.

    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Previously, two pathways were known to convert deoxycytidine nucleotides to dUMP for thymidine biosynthesis. Eukaryotes and some microorganisms dephosphorylate deoxycytidine nucleotides to form dCMP and then deaminate the nucleotide monophosphate using a zinc-dependent deaminase (15, 29). This pathway does not produce dUTP, so the cells need only sequester dCMP and dUMP from pyrimidine nucleotide monophosphate kinases to avoid futile cycles. On the other hand, many bacteria use the DCD protein to deaminate dCTP and the DUT protein to rapidly hydrolyze dUTP to form dUMP. These organisms require DUT proteins with high affinity for dUTP to avoid accumulating that toxic intermediate (Table III). M. jannaschii has circumvented the problem of dUTP accumulation by directly coupling dCTP deamination to the release of pyrophosphate using the MjDCD-DUT enzyme described here. This strategy of sequestering dUTP is similar to the use of multifunctional, substrate channeling enzyme complexes by eukaryotes to catalyze the initial steps in pyrimidine biosynthesis (30, 31).

Like the S. typhimurium DCD protein, MjDCD-DUT activity is inhibited by dTTP (15). Although the mechanism of this inhibition is not yet clear, dTTP concentrations may regulate the pathway by feedback inhibition. While some DUT proteins are inhibited by dTTP, they hydrolyze dTTP and dCTP with much lower efficiency than dUTP (12). Therefore members of the DCD/DUT family effectively discriminate among pyrimidine nucleotides.

Having a bifunctional enzyme that sequesters the dUTP intermediate does not relieve M. jannaschii of the need to hydrolyze free dUTP produced by spontaneous deamination and pyrimidine nucleotide kinase activity. Therefore that organism also has a dedicated dUTP diphosphatase. Although M. jannaschii DUT has a higher affinity for dUTP than does MjDCD-DUT, its affinity is still much lower than that of the E. coli or human DUT enzymes (Table III). If M. jannaschii DUT has similar kinetics in vivo, then the cells may require relatively high concentrations of DUT to maintain low dUTP levels during DNA replication.

Although the order of the hydrolytic reactions has yet to be determined, MjDCD-DUT's ability to hydrolyze dUTP to dUMP and pyrophosphate, but not to deaminate dCMP to dUMP, indicates that the reaction likely proceeds in the order of dCTP right-arrow dUTP right-arrow dUMP. Similar metal activation profiles for dCTP deaminase and dUTP diphosphatase activities, along with the results of a competition assay, suggest that deamination and pyrophosphate release occur at the same binding site. The fact that MjDCD-DUT E145Q protein has no deaminase activity but can still catalyze the release of pyrophosphate supports a model of both enzymatic reactions occurring at the same substrate binding site, but independently.

Both the affinity for dCTP and the turnover of MjDCD-DUT are much higher than those of the homologous S. typhimurium dCTP deaminase (Table III). In contrast to the cooperative kinetics observed for the S. typhimurium DCD enzyme, MjDCD-DUT kinetics show no sigmoidal relationship between dCTP concentration and the rate of deamination. On the other hand, both enzymes require divalent cation metals and the effective substrate for the enzymes is likely a Mg2+-dCTP complex. Unlike the cytidine deaminase (26) or adenosine deaminase (32, 33), MjDCD-DUT does not use a zinc-activated hydroxide to attack C-4 of the cytosine ring. The analogous mechanism of hydroxide activation by MjDCD-DUT is not yet known; however, the enzyme may use either an activated Mg2+-H2O nucleophilic complex or the Glu145 side chain carboxylate could serve as a general base, abstracting a proton from water. The loss of deamination activity in the MjDCD-DUT E145Q variant protein indicates that this residue is essential for deaminase activity. This glutamate residue is conserved in all DCD homologs, but not in most DUT homologs, except for crenarchaeal DUT proteins (Fig. 3). Therefore Glu145 is necessary but may not be sufficient for deaminase activity. In addition, we found that residues in motif 5 of DCD proteins differ from those in DUT homologs. In DUT crystal structure models, positions of this glycine-rich carboxy-terminal domain vary. Only in substrate-bound human and viral DUT structures did this domain become ordered (in a closed enzyme conformation), positioning the residues near the active site (12, 34). Additional studies will be needed to determine the contribution of this domain to the MjDCD-DUT enzyme's activities.

Unlike the bacterial dCTP deaminase, the MjDCD-DUT enzyme also has dUTP diphosphatase activity. Crystal structure models of DUT show that each of three active sites in the homotrimer is comprised of residues at the interface of all three subunits. One conserved acidic residue, equivalent to Asp135 of MjDCD-DUT, forms hydrogen bonds to tightly bound water molecules in the active site. It has been suggested that this residue is also involved in catalysis, activating water by proton abstraction (35, 36). A structural model of equine infectious anemia virus DUT shows that the analogous aspartate side chain forms a hydrogen bond with a main chain amide group from an adjacent subunit, stabilizing the protein's oligomeric structure (14). The variant protein MjDCD-DUT D135N is unable to catalyze either the dCTP deaminase or the dUTP diphosphatase reaction. Because results from gel-filtration chromatography suggest that the altered protein does not adopt the same quaternary structure as the wild-type enzyme, the specific role of Asp135 remains to be determined.

A molecular phylogeny of the DUT and DCD homologs implies that dCTP deaminase activity evolved from a dUTP diphosphatase after a single gene duplication event (10). Although both dut and dcd genes have been vertically inherited in many organismal lineages, phylogenies indicate numerous instances of horizontal gene transfer, which may have been mediated by viral vectors (37). Yet these phylogenies did not explain the evolution of deaminase activity in DCD, presumably from an ancestral gene encoding a dUTP diphosphatase. The bifunctional activity of the MjDCD-DUT protein described here illustrates how a superfamily of enzymes sharing a common partial reaction has evolved into a suprafamily of enzymes that catalyze mechanistically distinct reactions (38).

    ACKNOWLEDGEMENTS

We thank Kim Harich for assistance with EI-MS analysis and Marion Graupner for some early work on enzymatic assays.

    FOOTNOTES

* This work was supported by National Science Foundation (NSF) Grant MCB9985712 (to R. H. W.). Additional support was provided by an NSF grant awarded in 2002 (to D. E. G.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed. Tel.: 540-231-6605; Fax: 540-231-9070; E-mail: rhwhite@vt.edu.

Published, JBC Papers in Press, January 21, 2003, DOI 10.1074/jbc.M212460200

    ABBREVIATIONS

The abbreviations used are: DCD, dCTP deaminase; DUT, dUTP diphosphatase; PPi, pyrophosphate; HEPPS, N-(2-hydroxyethyl)piperazine-N'-3-propanesulfonic acid; CHES, 2-(N-cyclohexylamino)ethanesulfonic acid; MOPS, 3-(N-morpholino)propanesulfonic acid; MALDI-TOF, matrix-assisted laser desorption/ionization-time of flight; TMS, trimethylsilyl; EI-MS, electron impact-mass spectrometry.

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