From the
Department of Biochemistry and
McGill Cancer Center, McGill University, Montreal, Quebec H3G 1Y6, Canada
Received for publication, March 21, 2003 , and in revised form, April 28, 2003.
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ABSTRACT |
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INTRODUCTION |
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A second pro-apoptotic sub-group of the BCL-2 family, the BH3 domain-only members, couples upstream death signals to downstream activation of BAX/BAK (for review, see Ref. 14). Genes for some of these BH3 only proteins respond to transcriptional cues, yielding the active entity (e.g. Noxa, PUMA, and BIK) (1518). Others, however, are constitutively expressed and undergo an activating conformational change in response to a death stimulus (e.g. BAD, BIM, and BID) (1923), which is predicted to make available the BH3 helix. BID is cleaved by caspase-8 to tBID after death receptor stimulation and moves to mitochondria, where it interacts with and induces oligomerization of BAX and BAK (9, 10, 24). Although direct interactions between most other BH3 domain-only members and either BAX or BAK have not been reported, BH3-only members such as BAD also stimulate BAX/BAK as the death effectors (25, 26) by binding BCL-2 and sensitizing BAX/BAK to other stimuli, including tBID itself (27). Multidomain BCL proteins contain a deep groove formed by BH1 and BH2, which accepts the BH3 domain of a binding partner (28). In instances where anti-apoptotic BCL-2/BCL-XL is in excess, one model holds that these members bind to and sequester BH3-only proteins via their exposed BH3 helix, preventing them from stimulating the BAX/BAK effectors (25). Another model suggests that BH3-only proteins bind anti-apoptotic BCL-2 proteins, preventing them from inhibiting the BAX/BAK effectors (26). A third model, proposed on the basis of the anti-apoptotic function of adenovirus E1B 19-kDa protein, argues that the protein preferentially binds and inhibits a conformationally altered form of BAX in cells after receipt of a death stimulus (24). Which of these pathways dominates may in fact depend on the 3-way relative expression levels of anti-apoptotic BCL members, pro-apoptotic BAX, BAK, and the active BH3-only entities that initiate mitochondrial apoptosis.
A recent study suggests a two-step mechanism for mitochondrial release of cytochrome c in response to tBID; that is, BH3-independent mobilization of intra-cristae cytochrome c stores and a BH3-dependent egress of cytochrome c from the organelle associated with BH3-stimulated oligomerization of BAK (29). Documentation of this latter step accompanied by findings from mutagenesis in which the tBID mitochondrial targeting sequence was replaced with that of BCL-2 argue that simple concentration of the tBID BH3 domain at the surface of mitochondria is sufficient to initiate BAK oligomerization in the outer membrane (10). Furthermore, generation of tBID by caspase cleavage involves exposure of its BH3 domain (30, 31) and a freely diffusing synthetic peptide corresponding to the tBID BH3 domain stimulates BAK oligomerization in the mitochondrial outer membrane and release of cytochrome c (27). Use of the isolated BH3 domain in the form of a pure oligopeptide offers certain advantages, such as biotinylation and directional cross-linking, for probing directly the molecular interactions that mediate these BH3-dependent events. Outstanding issues, for example, relate to the nature of the BAK oligomerization pathway itself and the opposing roles of tBID and BCL-2 in its regulation.
Here, we have utilized derivatized oligo-peptides corresponding to the entire helix 3 of BID to study these questions and find that this BH3-containing peptide binds BAK, resulting in exposure of the BAK N terminus. This open conformer of BAK itself potently induces oligomerization of non-activated (closed) BAK members in the outer membrane, indicative of an amplification cascade. BCL-2, at levels that block cytochrome c egress from mitochondria, does not interfere with binding of BID BH3 to BAK or induction of the open conformer of BAK, but it selectively interacts with the tBID BH3-stimulated (open) BAK conformer, inhibiting BAK oligomerization and cytochrome c release.
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EXPERIMENTAL PROCEDURES |
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PeptidesThe wt1 and mt BID BH3 peptides were generated by the Alberta Peptide Institute (University of Alberta, Edmonton, Alberta, Canada) and represent amino acids 79101 of BID. Both are biotinylated at their N termini and contain an Arg to Lys substitution at position 88 and an amide in place of a carboxyl at the C terminus. The mutant peptide also contains a Leu to Gly substitution at position 90. The corresponding wild-type BAD and BIK BH3 peptides were also synthesized. All peptides were purified (>95%) by high performance liquid chromatography.
BAK Translation ProductsTwo separate BAK constructs were engineered for in vitro assays, both of which contain a FLAG epitope at their N termini. FLAG-BAK-N(C to A) has a deletion of the N-terminal 36 amino acids and a substitution of its single Cys to Ala at position 166. FLAG-BAK(C to A) comprises full-length BAK with substitutions of both of its Cys residues at positions 14 and 166 to Ala. The constructs were cloned into pcDNA3.1 and in vitro transcribed and translated using the TNT® rabbit reticulocyte lysate system (Promega) as per the manufacturer's protocol for non-radiolabeled translations.
Release of Cytochrome c from MitochondriaMitochondria (50 µg of protein in 25 µl of cMRM (250 mM sucrose, 10 mM HEPES, 1 mM ATP(K+), 5 mM sodium succinate, 0.08 mM ADP, 2 mM K2HPO4)) isolated from control (HA-BCL-2) KB or from KB cells overexpressing HA-BCL-2 (39) were incubated with 20 µl of HIM (200 mM mannitol, 70 mM sucrose, 10 mM HEPES-KOH, 1 mM EGTA, pH 7.5) or 20 µl of HeLa cytosolic extract in HIM (4 mg protein/ml) as described (3, 32) plus or minus recombinant caspase-8 (100 ng in 1 µl of Tris-HCl). Alternatively, the mitochondria were incubated with recombinant tBID (in 5 µl of phosphate-buffered saline) (22), synthetic BH3 peptides (in 5 µl of 0.5 mM Pipes, pH 6), BAK translation product, or control buffer (0.5 mM Pipes, pH 6), adjusted to a final volume of 50 µl with HIM, and incubated for 1 h at 37 °C (3, 32). The reaction mixtures were then centrifuged at 9000 rpm (Sorvall MC 12V), and equivalent aliquots of both the supernatants and pellets (resuspended to the same volume as the supernatant) were analyzed by SDS-PAGE and immunoblot using the mouse monoclonal anti-pigeon cytochrome c antibody 7H8.2C12 (Pharmingen).
BAK OligomerizationReactions were conducted as for release of cytochrome c from mitochondria, except that the mitochondria (50 µg) that were recovered at the end of the reaction were resuspended in 47.5 µl of HIM. Subsequently, 2.5 µl of Me2SO (control) or 2.5 µl of a solution of bismaleimidohexane (BMH) (Pierce) in Me2SO were added and incubated at room temperature for 30 min. The reactions were centrifuged at 12,000 rpm (Sorvall MC 12V), and the pellets were analyzed by SDS-PAGE and immunoblot using a rabbit polyclonal antibody directed against amino acids 2337 of human BAK (Upstate).
Treatment of BAK with Trypsin5 µl of MRM (250 mM sucrose, 10 mM HEPES, pH 7.5) containing trypsin (0.125 mg/ml, Sigma) and 5 µl of MRM with (mock) or without chymotrypsin-trypsin inhibitor (1.25 mg/ml, Sigma) were added directly to reaction mixtures, which were then incubated on ice for 20 min. The trypsin digestion was stopped by the subsequent addition of 5 µl of the inhibitor, the mitochondria were recovered at 9000 rpm (Sorvall MC 12V) for 5 min through a cushion of MRM, and BAK was analyzed by SDS-PAGE and immunoblot as above.
Directional Cross-linkingBiotinylated peptides were incubated in the presence of excess succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxy-(6-amidocaproate) (LC-SMCC, 10 mM stock in Me2SO, Pierce), an amine/sulfhydryl hetero-bifunctional cross-linker, for 1 h at room temperature The amine reactive group on any excess cross-linker was then quenched by the addition of Tris-HCl, pH 8, to a final concentration of 350 mM followed by incubation at room temperature for 15 min. The derivatized peptides were then added to 50-µl standard reaction mixtures containing mitochondria. After 1 h at 37 °C, the mitochondria were recovered and solubilized in 200 µl of RIPA buffer (10 mM Tris, pH 7.4, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 1% deoxycholate, and 0.1% SDS). 20 µl of a 50% slurry of Immunopure® immobilized streptavidin beads (Pierce) were then added, and the mixtures were incubated overnight at 4 °C. The beads were recovered, washed, resuspended in SDS-PAGE loading buffer, and analyzed for BAK or BCL-2 by SDS-PAGE and immunoblot using anti-BAK or the monoclonal hamster anti-human BCL-2 antibody 6C8 (Biomol).
ImmunoprecipitationMitochondria from HA-BCL-2-overexpressing cells were incubated in standard cross-linking reactions in the absence of detergent, recovered, and solubilized in 200 µl of RIPA buffer. The monoclonal mouse anti-HA antibody 16B12 (Berkeley Antibody Co.) was then added and incubated overnight at 4 °C. 20 µl of a 50% slurry of protein G-Sepharose (Amersham Biosciences) were added to the samples and incubated at 4 °C for 1 h. The beads were washed and analyzed for the presence of BAK and BCL-2 as above.
Analysis of BCL-2 and BAK Interaction in Fas-stimulated KB Cells Human KB epithelial cells overexpressing HA-BCL-2 were treated with Fas-ligating anti-Fas antibody (0.5 µg of protein/ml; Upstate, CH11), as described (38). At the indicated times, mitochondria were isolated, aliquots (100 µg protein) were treated with the cross-linker BMH, HA-Bcl-2 was recovered after protein dissolution in RIPA buffer with anti-HA, and precipitates were analyzed with antibodies against BCL-2 and BAK as described above.
Transfection and ImmunofluorescenceControl and HA-Bcl-2-overexpressing KB cells were plated to 50% confluency on 12-mm coverslips in 24-well plates and transfected overnight with a combination of pEGFP, pIND-tBID-GFP, and pVgRXR (22) in a 2:4:4 ratio using LipofectAMINE PLUSTM (Invitrogen) as per the manufacturer's protocol. The expression of tBID was then induced by treating the cells with 1 µM ponasterone A (Invitrogen) in growth medium. At the indicated times, the cells were fixed, and immunofluorescence was performed as described in Ruffolo et al. (32) using either a mouse monoclonal anti-cytochrome c antibody (BD Biosciences, 6H2.B4) or a mouse monoclonal BAK conformation-specific antibody directed to the N terminus of BAK (Oncogene, TC100) as the primary antibody. Alexa Fluor® 594 goat anti-mouse secondary antibody (Molecular Probes) was used to visualize cytochrome c and the reactive BAK conformation. Transfected cells expressing EGFP were scored for the release of cytochrome c from mitochondria to cytosol and for staining of the conformation-specific, exposed BAK N terminus.
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RESULTS AND DISCUSSION |
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BID BH3 Peptide Recapitulates tBID or the Treatment of Cytosol with Caspase-8 When the combined cytosol-mitochondria reaction mixtures were subsequently centrifuged to pellet the organelle, cytochrome c was observed in the supernatant only if the cytosol had been treated with caspase-8 (Fig. 1D, lanes 3 and 4). Mitochondria isolated from KB cells overexpressing HA-BCL-2, on the other hand, resisted this caspase-8-induced release of cytochrome c (Fig. 1D, lanes 1 and 2). As expected, supplementing the HeLa cell cytosol with 0.06 µM recombinant tBID (22, 32) bypassed the requirement for treatment of the cytosol with caspase-8, and the control mitochondria (HA-BCL-2) effectively released cytochrome c (lane 5). An equivalent 0.06 µM concentration of synthetic peptide corresponding to the 23-amino acid helix 3 of BID, which contains the 15-amino acid BH3 domain (Fig. 1B), also stimulated release of cytochrome c from control mitochondria (lane 6), but concentrations of peptide that were 510-fold higher than the equivalent concentration of recombinant tBID were required for a comparable amount of cytochrome c release (compare lane 5 with lanes 6 and 7). This differential is consistent with a membrane-targeting sequence present in tBID but lacking in the synthetic peptide that serves to concentrate tBID at the mitochondria (10). Of note, a mutant peptide in which the highly conserved Leu present in all BH3 domains examined to date (amino acid 90 in BID) was changed to Gly completely abrogated the ability of the BID BH3 peptide to cause cytochrome c release from the organelle (Fig. 1D, lanes 911). Also, as expected, cytosol had a negligible effect on the ability of the wt BID BH3 peptide to induce cytochrome c release in this assay (not shown).
As anticipated, mitochondria from HA-BCL-2 overexpressing cells (inset, Fig. 1E) resisted the induction of cytochrome c release from the organelle both by recombinant tBID (not shown) and by the synthetic BH3 peptide (Fig. 1E, conducted in the absence of cytosol). This protection by HA-BCL-2, however, could be overcome by increasing the concentration of BH3 peptide (a representative experimental result is shown in Fig. 1E). The ratio of BCL-2 and BH3 peptide, therefore, appears to determine the sensitivity of the mitochondria to cytochrome c release (see also Letai et al. (27)). Mitochondria from cells overexpressing HA-BCL-2 were able to resist the effects of BID BH3 up to about 10 µM peptide (Fig. 1E) and of tBID up to about 1 µM (not shown). A corresponding resistance was also observed toward the ability of the BH3 peptide to induce BAK oligomerization in the mitochondrial outer membrane (Fig 2A, compare lanes 35 with lanes 1012), which was visualized as high molecular weight anti-BAK immunoreactive products detected by SDS-PAGE after chemical cross-linking of mitochondria with the sulfhydryl-specific homo-bifunctional BMH (10). Interestingly, at the peptide concentrations used, both BAK oligomerization (Fig. 2A) and cytochrome c release from mitochondria (Fig. 1F) were much more responsive to the BID BH3 peptide compared with BH3 peptides of similar size and design derived from BAD and BIK (see also Letai et al. (27)). This may reflect the observation that among BH3-only proteins, tBID is unique in that it yields detectable interactions with pro-apoptotic BAX and BAK (33).
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BID BH3 Interacts with BAK and Induces an Open Conformation, Exposing the BAK N TerminusInspection of the BAK sequence reveals the presence of two cysteine residues, the first lying toward the N terminus at amino acid position 14, and the second, at position 166, within the BH2 domain (Fig. 1A). As noted by Wei et al. (10), treatment of non-induced mitochondria with BMH results in intramolecular cross-linking of these two cysteine moieties in BAK, generating a BAK species that migrates in SDS-PAGE with a faster mobility than unmodified BAK (double asterisk in Fig. 2A). This intramolecular cross-linked species was lost in mitochondria that were stimulated with the BID BH3 peptide, whereas high molecular weight cross-linked BAK oligomers appeared (Fig. 2A, lanes 35). A similar pattern was observed in intact cells after Fas stimulation (not shown). Although one explanation is that the cysteine residues in BH3-stimulated BAK preferentially engage in intermolecular cross linking, this explanation does not account for the observation that in the presence of excess BCL-2, BAK oligomerization was inhibited, yet the loss of intramolecular cross-linking was still observed after treatment with the BID BH3 peptide (Fig. 2A, lanes 1012). Another explanation, therefore, is that BH3-stimulated BAK undergoes a change that precludes intramolecular cross-linking between Cys-14 and Cys-166, even in the presence of BCL-2.
As shown in Fig. 2B, the addition of wt but not mt BID BH3 peptide to mitochondria caused BAK to undergo a conformational change, in which trypsin-resistant BAK became susceptible to the protease and resulted in loss of reactivity of BAK toward an antibody directed toward the BAK N terminus (amino acids 2735). This indicates that in its unstimulated form, BAK is in a closed conformation with its N terminus inaccessible (34, 35), and this corresponds to the form of BAK whose N-terminal Cys is susceptible to intramolecular cross-linking by BMH to the Cys residue within the BH2 helix (Figs. 1A and 2A). Importantly, HA-BCL-2 in excess did not inhibit the ability of either 3.75 µM BID BH3 peptide or 0.15 µM recombinant tBID to induce exposure of the BAK N terminus (Fig. 2B), whereas BCL-2 did inhibit both reagents from inducing BAK oligomerization (Fig. 2A, lanes 1012; not shown) and cytochrome c release (Fig. 1E; not shown). Therefore, BCL-2 blocks tBID-induced cytochrome c release from mitochondria downstream of tBID induction of the BAK open (trypsin-sensitive) conformation.
The BID BH3-dependent change at the N terminus of BAK correlated with a direct interaction between BAK and the BID BH3 peptide. Such interaction was demonstrated by first covalently attaching the hetero-bifunctional (amino/sulfhydryl) cross-linker, LC-SMCC, to the single Lys residue in the peptide (Fig. 3A). This Lys was introduced in place of Arg-88, which normally resides at this position in the wt sequence. This change did not affect the ability of tBID to induce apoptosis in transfected cells (not shown) and, therefore, was considered to be a silent change. 3.75 µM biotinylated wt or mt (Leu to Gly) peptide-LC-SMCC complex was incubated with mitochondria in the absence of detergent, and covalent adducts to 1 of the 2 Cys residues in BAK (Fig. 1A) were recovered after solubilization and vigorous washing in RIPA buffer containing streptavidin beads. Only the wt biotinylated BH3 peptide formed a covalent complex with BAK (Fig. 3B, upper panel). Of note, BAK-BH3-cross-linked adducts were generated in mitochondria lacking or harboring excess HA-BCL-2 (Fig. 3B, lower panel), indicating that BCL-2 does not interfere with the specific interactions of wt BID BH3 with BAK despite the fact that HA-BCL-2 strongly inhibited BID BH3-induced BAK oligomerization (Fig. 2A) and release of cytochrome c from the organelle (Fig. 1, D and E). Furthermore, this inability of HA-BCL-2 to block interactions of BID BH3 with BAK occurred even though the peptide also interacted with HA-BCL-2 (Fig. 3C). This binding to BCL-2, therefore, does not result in functional sequestration of BID BH3 from BAK and suggests that the binding equilibria at the mitochondrial surface do not favor BH3-BCL-2 interactions.
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BCL-2 Preferentially Interacts with BID BH3-stimulated BAKInteractions between BAK and BCL-2 in mitochondria isolated from HA-BCL-2 overexpressing KB cells were assessed by chemical cross-linking with BMH followed by protein dissolution, precipitation with anti-HA, and immunoblotting of precipitates with anti-BAK. It is particularly noteworthy that cross-linking between HA-BCL-2 and BAK above background was not observed in the absence of the BID BH3 stimulation (Fig. 4A). Specific covalent adducts between HA-BCL-2 and BAK were observed, on the other hand, after stimulation with wt but not mt BH3 peptide (Fig. 4A, upper panel). Collectively, therefore, the results show that BID BH3 physically interacts with BAK at the surface of mitochondria (Fig. 3B) and induces a conformational change in which the BAK-N terminus becomes accessible to trypsin (Fig. 2B); BCL-2 preferentially interacts with this BH3-stimulated open conformation of BAK (Fig. 4A) and inhibits BH3-induced BAK oligomerization (Fig. 2A) and cytochrome c release dependent on the ratio of BID BH3 and BCL-2 (Fig. 1E).
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A Cascade of BAK Auto-oligomerizationAlthough tBID initiates BAK oligomerization, the mechanism for how this is accomplished is not known. The fact that BID BH3 caused BAK to adopt an open (i.e. trypsin accessible) conformation even in the presence of excess BCL-2 (Fig. 2B) suggests that BCL-2 functions downstream of this step to inhibit both BAK oligomerization (Fig. 2A) and release of cytochrome c from mitochondria (Fig. 1, D and E). One possibility is that the unstimulated (closed) conformation of BAK is incompetent for auto-oligomerization and that BID BH3, by inducing exposure of the N terminus, relieves this barrier. To test this idea, a BAK construct was generated that lacks amino acids 136 (FLAG-N-BAK, see Fig. 1A), and its ability to induce oligomerization of endogenous BAK in isolated mitochondria was assessed. Because oligomerization is assayed by cross-linking with the Cys-specific homo-bifunctional BMH cross-linker, both the full-length and
N-BAK constructs that were used as the "donor" proteins were further modified to change their constituent Cys residues to Ala (Fig. 1A). In this way, the donor BAK molecules cannot be visualized as part of the oligomeric complex, whereas oligomerization of endogenous "acceptor" BAK molecules can. As documented in Fig. 4B, the transcription-translation product of FLAG-
N-BAK(C to A) stimulated oligomerization of endogenous mitochondrial BAK and release of cytochrome c from the organelle, whereas full-length FLAG-BAK(C to A) did not. The latter construct, as expected, was resistant to degradation of its N terminus by trypsin (Fig. 4B, right panel). Furthermore, mitochondria containing excess HA-BCL-2 (Fig. 1, CE, and 2A) resisted BAK oligomerization and cytochrome c release in response to FLAG-
N-BAK(C to A) (not shown). Because the antibody that was used to detect BAK oligomers recognizes the BAK N terminus, which is lacking in the FLAG-
N-BAK(C to A) translation product, the observed oligomers must have derived from the endogenous population of mitochondrial BAK.
BCL-2 Selectively Interacts with Death-stimulated BAK in Intact CellsThe results presented in Fig. 5 show that the key findings presented above utilizing isolated mitochondria in vitro can be recapitulated in intact human KB epithelial cells stimulated with tBID or Fas ligation. tBID-initiated changes in BAK conformation and cytochrome c distribution was achieved by co-transfecting a vector expressing EGFP and a vector conditionally expressing tBID under the control of an inducible promoter (22, 32). After induction of tBID expression with ponasterone A, EGFP-positive cells were probed by immunofluorescence using an antibody against cytochrome c and an antibody directed to the N terminus of BAK; the latter selectively recognizes a specific conformation of BAK with an exposed N terminus (34). As shown in Fig. 5, A and B, left panel, tBID induced the release of cytochrome c in control cells but not in cells overexpressing HA-BCL-2 (see the inset, Fig. 1E). Excess BCL-2 had no effect, however, on the ability of tBID to activate the conformationally altered BAK with the exposed N terminus (Fig. 5, A and B, right panel), consistent with the findings in vitro that BCL-2 functions downstream of the tBID-induced activated conformer of BAK to restrict loss of cytochrome c from the organelle (Fig. 2B). Moreover, the previous finding that BID BH3 peptide induces BAK/BCL-2 interaction in isolated mitochondria (Fig. 4A) argues that these interactions occur in the presence but not absence of cell death stimuli that are coupled to BID. To test this, KB cells overexpressing HA-BCL-2 were stimulated for 0, 2, or 3 h with agonistic anti-Fas antibody, and the mitochondria were isolated and subjected to cross-linking with BMH, after which proteins were dissolved in RIPA buffer and subjected to BCL-2 pull down with anti-HA, and the precipitates were probed by immunoblot with antibody against BCL-2 or BAK. HA-BCL-2/BAK heterodimers were observed after but not before stimulation of the cells by Fas ligation (Fig. 5C).
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Concluding RemarksAltogether, the findings presented here suggest a model (Fig. 6) in which the BAK N terminus represses the ability of unstimulated BAK to undergo spontaneous self-oligomerization in the mitochondrial outer membrane. By binding to BAK, the BID BH3 domain overcomes this barrier. Furthermore, the resulting open (N terminus exposed) BAK conformer can induce oligomerization of closed BAK conformers, indicative of a BID BH3-initiated cascade of BAK auto-oligomerization and cytochrome c release from mitochondria. BCL-2 preferentially interacts with the BID BH3-induced open conformer of BAK, blocking this cascade. This model might also extend to BAX since the adenovirus BCL-2 member, the E1B 19-kDa protein, appears to selectively bind the BAX open conformer (8, 24, 35, 36). By interacting with the BID BH3-stimulated open BAK conformer, BCL-2 can intercede and inhibit BID BH3 signaling by selectively targeting the initiation step of the BAK oligomerization cascade, consistent with the model proposed for the E1B 19-kDa protein (8, 24). Excess BCL-2, at levels that can inhibit cytochrome c release from mitochondria, does not prevent BID BH3 from binding BAK, indicating that BCL-2, at levels that can inhibit cytochrome c release from mitochondria, does not sequester BID BH3 nor prevent it from directly activating BAK. Sequestration of BID BH3 by BCL-2, of course, would occur at further elevated BCL-2, where BCL-2 is sufficiently in excess relative to BAK that it represents an overwhelming sink for tBID (25). Conversely, the fact that BID BH3 in excess can bind to both BAK and BCL-2, however, suggests a two-step mechanism for BID BH3-induced oligomerization of BAK; interaction of BID BH3 with BAK to induces the open conformation and interaction with BCL-2 to prevent BCL-2 from binding and inhibiting this active BAK conformer (Fig. 6). Consistent with this idea, subsequent BAK oligomerization and release of cytochrome c from mitochondria is dependent on the ratio of pro-apoptotic BID BH3 and anti-apoptotic BCL-2. Moreover, this model suggests that BH3-only proteins that interact with BCL-2 but not with BAK may require a co-stimulus to induce the open BAK conformer to initiate the mitochondrial pathway of apoptosis (27).
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Finally, recent studies (13) suggest that the critical path for BAX-dependent egress of macromolecules such as cytochrome c across a membrane lipid bilayer can be reconstituted by combining just BAX and tBID in the appropriate lipid environment. Likewise, mitochondria isolated from cellular homogenates, such as those described here, are also responsive. It must be emphasized, however, that in intact cells undergoing apoptotic transitions, barriers that help to maintain the integrity of the mitochondrial network are probably removed by parallel pathways. For example, important transformations have been identified that sensitize mitochondria to a BH3-only hit, such as tBID, including mitochondrial fission and fragmentation induced by endoplasmic reticulum calcium-mediated pathways (37).
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FOOTNOTES |
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¶ To whom correspondence should be addressed: 3655 Promenade Sir William-Osler, Rm. 906B, Montreal, Quebec H3G 1Y6, Canada. Tel.: 514-398-7282; Fax: 514-398-7384; E-mail: gordon.shore{at}mcgill.ca.
1 The abbreviations used are: wt, wild type; mt, mutant; Pipes, 1,4-piperazinediethanesulfonic acid; BMH, bismaleimidohexane; LC-SMCC, succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxy-(6-amidocaproate; RIPA, radioimmune precipitation assay buffer; HA, hemagglutinin; EGFP, enhanced green fluorescent protein.
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ACKNOWLEDGMENTS |
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REFERENCES |
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