From the INSERM U538, Centre Hospitalo-Universitaire Saint Antoine. 27 rue de Chaligny, 75571 Paris Cedex 12, France
Received for publication, March 6, 2003
, and in revised form, April 7, 2003.
![]() |
ABSTRACT |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
![]() |
INTRODUCTION |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Different Hsps are found in different cellular compartments and organelles. Among the major Hsps currently identified, the stress-inducible 72-kDa Hsp70 or the constitutively expressed 73-kDa Hsc70 are mainly found in the cytosol, but following stress they migrate to the nucleus and associate to nuclear proteins (2, 3). Hsps also include a group of analogous proteins confined to the endoplasmic reticulum (ER). Among these are the 78-kDa glucose-regulated protein (Grp78) located in the ER lumen and the calcium-binding proteins calreticulin and calnexin, the luminal and transmembrane-associated ER Hsps, respectively. There is recurrent evidence suggesting that several Hsps (ER-resident and cytosolic) are also expressed at the cell membrane (47). The origin of membrane-associated Hsps still remains elusive, and it has recently been suggested that they may also be secreted by cells (811) before being associated with the external leaflet of cell membranes. Membrane-associated or secreted Hsps (Hsp70 in particular) have been suggested to exert a necessary, but still poorly understood, role in the immune response and signal transduction (79, 12, 13). However, there is still no available data on the mechanisms by which Hsp70 might be released from cells. Since Hsp70 and some other Hsps are intracellular proteins and lack any secretory signal sequence, one should hypothesize that these proteins are not released through the classical exocytic pathway.
Our initial studies, aimed at defining the nature of the membranes to which Hsp70 may attach, have suggested that specialized membrane microdomains may be involved. These microdomains, also termed lipid rafts, are formed within the exoplasmic leaflet of the Golgi membrane (14, 15). Lipid rafts are characterized by their resistance to non-ionic detergent extraction at low temperature combined with their ability to float during density gradient centrifugation (16). These detergent-resistant microdomains (DRMs) are enriched in cholesterol, glycosphingolipids, glycosylphosphatidylinositol-anchored proteins, and some other acylated or transmembrane proteins (16).
In the present study, a model of cultured human intestinal cells, namely Caco-2, was used to study the expression and membrane association of Hsps. Different Hsps were found to associate with DRMs to a variable extent depending on the particular Hsp considered, and association of Hsps with these microdomains could be modulated by stress. DRM fractions obtained from unstressed cells contained consistent levels of Hsp70, while Hsc70 and ER chaperone levels were very low or undetectable. The stress response (heat shock, depletion of ER calcium, and inhibition of protein trafficking) resulted in an increased accumulation of some Hsps into DRM fractions as compared with unstressed cells. Notably, in stressed Caco-2 cells, the amount of Hsp70 was selectively increased in DRMs, and this was correlated with a robust stimulation of Hsp70 release. Manipulating the lipid composition of DRMs resulted in a concomitant modulation of Hsp70 release, thus suggesting that lipid rafts may represent a cellular mechanism for membrane delivery and release of Hsps.
![]() |
EXPERIMENTAL PROCEDURES |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
The antibodies used were as follows: mouse anti-Hsp70 monoclonal antibody (catalog no. SPA-810), rat anti-Hsc70 monoclonal antibody (catalog no. SPA-815), mouse anti-calreticulin monoclonal antibody (catalog no. SPA-601), mouse anti-KDEL monoclonal antibody that recognizes Grp78 (catalog no. SPA-827), and rabbit anti-calnexin polyclonal antibody (catalog no. SPA-860) (StressGen, Victoria, Canada). Peroxidase-conjugated secondary antibodies, fluorescein isothiocyanate-conjugated F(ab')2 fragments of rabbit anti-mouse IgG, and goat anti-rabbit IgG were from Dako (Glostrup, Denmark). The Hsp70 enzyme-linked immunosorbent assay (ELISA) kit (catalog no. EKS-700) was from StressGen.
Cell Culture
Human colonic adenocarcinoma (Caco-2) cells (passage 5080) were cultured in Dulbecco's modified Eagle's medium supplemented with 20% fetal calf serum, 1% penicillin-streptomycin, and 1% non-essential amino acids. Cells were plated 21 days before experiments and maintained at 37 °C in 10% CO2. These conditions allow differentiation of epithelial membrane into apical (brush border) and basolateral domains. Some experiments were performed with a non-tumoral epithelial cell line, Madin-Darby canine kidney, cultured as confluent monolayers in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum and 1% penicillin-streptomycin.
Heat Shock
Cells were exposed to thermal stress for 30 min in culture flasks partially immersed in a thermostatically regulated water bath. Cells were allowed to recover at 37 °C in 10% CO2 to allow the synthesis of Hsps.
Pretreatments with Agents Perturbing Golgi or ER Functions and Protein Traffic
Tunicamycin (25 µg/ml), monensin (25 µM), brefeldin A (15 µM), and thapsigargin (0.5 µM) were added to Dulbecco's modified Eagle's medium 18 h before experiments in supplemented culture medium.
DRM Preparation
DRM preparation was adapted from a procedure described previously (17). Adherent cells (12 x 107/25-cm2 culture flask) were washed twice in PBS, lysed with 2 ml of extraction buffer (20 mM Tris-HCl, pH 7.4, NaCl 150 mM, EDTA 1 mM) supplemented with 1% Triton X-100 and a protease inhibitor mixture. Lysates were scraped from the flasks, sheared by 20 passages through a 22-gauge needle, left for 20 min before mixing with OptiPrep® density gradient medium (final concentration, 40% (v/v)), and placed at the bottom of a 12-ml ultracentrifuge tube. A discontinuous Optiprep gradient was formed by overlaying 4 ml of 30% Optiprep and 4 ml of 5% Optiprep (prepared by dilution of OptiPrep in extraction buffer). Gradients were ultracentrifuged at 100,000 x g for 4 h in a SW41 rotor (Beckman). A distinct Triton X-100-insoluble whitish band that floated to the 530% interface was designated as the DRM fraction. The last 4 ml of the gradient tube were mixed and designated as the soluble fraction. The whole procedure was performed at 0-4 °C. DRM and soluble fractions were collected and subjected to electrophoresis, Western blot, and ELISA analysis.
Electrophoresis and Western Blot
Cell proteins (5 µg/lane) were separated electrophoretically on one-dimensional SDS, 7.5% polyacrylamide gels under reducing conditions. Proteins of the gels were transferred to nitrocellulose membranes (Hybond, 0.45 µm, Amersham Biosciences) and probed with antibodies against Hsps (anti-Hsp70 diluted to 1:5,000, anti-Hsc70 diluted to 1:5,000, anti-KDEL diluted to 1:250, and anti-calreticulin and anti-calnexin diluted to 1:2,000 followed by anti-mouse or anti-rabbit peroxidase-conjugated antibodies diluted to 1:20,000). Blots were developed using ECL detection reagents according to the protocol supplied by the manufacturer. Immunoblots were scanned with a densitometric scanner (Arcus II, Agfa, Mortsel, Belgium) and quantified using Scion Image software.
Flow Cytometry
Analysis by flow cytometry was performed in cells detached with trypsin-EDTA, fixed with 3% paraformaldehyde, and permeabilized by 0.3% saponin. Primary antibodies were added for 18 h at 4 °C diluted at 1:100 (anti-Hsp70, anti-Hsc70, anti-calreticulin, and anti-calnexin) or diluted to 1:50 (anti-KDEL) in PBS containing 1% bovine serum albumin. Secondary antibodies were added for 1 h at room temperature in the dark: fluorescein isothiocyanate-conjugated rabbit anti-mouse IgG or fluorescein isothiocyanate-conjugated goat anti-rabbit IgG were diluted to 1:50 in PBS-bovine serum albumin. In nonspecific controls, cells were incubated with the secondary antibody alone. Fluorescence and light scatters were analyzed in a BD Biosciences fluorescence-activated cell sorter for green fluorescence detected through a 525-nm filter. Data are expressed as the percentage of positive cells above nonspecific controls.
Additional Methods
ELISAQuantitative comparisons between the expression of Hsp70 in DRM and soluble fractions of different preparations from normal and stressed cells were performed using a commercial ELISA kit as recommended by the manufacturer. This assay was also used to quantify Hsp70 expression in cell supernatants.
ProteinsProtein concentrations were determined using BCA protein assay reagent and bovine serum albumin as standard according to the manufacturer's instructions.
Cholesterol DepletionTo deplete cholesterol cells (12 x 107) were treated by methyl--cyclodextrin (10 mM) and rocked for6hat37 °C. To estimate cholesterol depletion cells were pretreated with 3 µCi/ml [3H]cholesterol for 16 h in complete culture medium. At the end of the incubation, aliquots of the supernatants were collected, and their radioactivity was counted by liquid scintillation (LS 6000 SC, Beckman). Cells were then washed twice in PBS and lysed for DRM preparation. The ability of methyl-
-cyclodextrin to deplete cholesterol from different fractions obtained was estimated according to their radioactivity measured by liquid scintillation.
Alkaline Phosphatase and Dipeptidyl-peptidase IV ActivitiesAlkaline phosphatase and dipeptidyl-peptidase IV activities were determined spectrophotometrically at 410 nm using p-nitrophenyl phosphate and glycyl-L-proline p-nitroanilide as substrate, respectively.
LDHLDH was determined spectrophotometrically at 340 nm using a commercial kit as recommended by the manufacturer.
StatisticsData are presented as mean ± S.E. Comparisons between groups were performed using the Wilcoxon's signed ranked test. A p value ≤0.05 was considered significant.
![]() |
RESULTS |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
|
|
|
Expression of Hsps in Membrane Microdomains of Epithelial Cells: Modulation by Heat Shock and Drugs That Interfere with Protein TrafficAs a first step toward the demonstration that Hsps may associate with epithelial cell membranes, flow cytometry experiments were performed on non-permeabilized cells. As shown in Table III, a significant amount of Hsp70 was detected in unpermeabilized Caco-2 cells that likely corresponded to a membrane staining. To further characterize this membrane association and since it has been suggested that, in monocytes, Hsp70 may associate with membrane microdomains (18), the distribution of Hsp70 in Caco-2 cells was studied on an Optiprep gradient after Triton X-100 extraction. As shown on Fig. 2A, this protocol allowed isolation of a DRM-enriched fraction (fraction 4) in which alkaline phosphatase, a glycosylphosphatidylinositol-anchored brush border protein known as a good raft marker, was strongly enriched (70.0 ± 0. 9%, n = 3) and soluble fractions (fractions 812) in which dipeptidyl-peptidase IV, a brush border hydrolase known to be mostly excluded from rafts (19), was almost exclusively recovered (94.0 ± 0.2%, n = 3). The expression of Hsp70 was determined in this gradient by Western blot (Fig. 2B), and a significant proportion of Hsp70 (14.6 ± 5.3%, n = 3) was detected in DRM fractions of untreated Caco-2 cells. This pattern of expression was also recovered from another epithelial cell line, Madin-Darby canine kidney, indicating that Hsp70 expression in rafts is not restricted to Caco-2 cells. The effect of heat shock on the level of expression and the distribution between the DRM and soluble fractions was dramatic. Besides the expected increase in total expression of Hsp70, a clear redistribution of Hsp70 from the soluble fractions to DRMs was observed as a function of temperatures from 37 to 45 °C (Fig. 2C), indicating that Hsp70 association to membrane microdomains is modulated by stress. Note that in this and the following experiments only two fractions were routinely considered: the DRM fraction 4 located at the interface (530%) and the soluble fraction that corresponded to a pool of fractions 812.
|
|
To further characterize this phenomenon, the level of expression and membrane distribution of different Hsps were studied in resting or stressed cells (Fig. 2D). Strikingly only two Hsps, namely Hsp70 and calnexin, displayed a redistribution to DRMs upon stress conditions, whatever the nature of the stress. The other Hsps displayed an expected overexpression without any redistribution. It is important to note that no change was observed in the distribution of alkaline phosphatase and dipeptidyl-peptidase IV between DRM and soluble fractions when cells were switched from a resting to a stressed situation (data not shown). The association of Hsp70 to DRMs was further confirmed using a quantitative ELISA protocol. Data from ELISA, described in Table IV, were consistent with those obtained by Western blot: Hsp70 is an abundant protein in Caco-2 cells (500 and 1,600 ng/mg of protein in control and heat-shocked Caco-2 cells, respectively). In unstressed cells, the amount of Hsp70 present on DRMs is only 17% of the total cellular content, most of which resides in the soluble fraction. Following heat shock, expression of Hsp70 was enriched in DRMs to reach 35% of the total cellular content (Table IV). We next examined whether association of Hsp70 to DRM might result in an extracellular localization of the protein.
|
DRM Localization of Hsp70 and Its Release Are Two Related EventsHsp70 was measured in cell supernatants by ELISA. Release of Hsp70 during a 6-h incubation period was 1.7 ± 0.4% of the total Hsp70 content in resting Caco-2 cells. Heat shock induced a high increase of Hsp70 release that increased up to 14.2 ± 2.2% of the total Hsp70 content (p < 0.01, n = 10) (Fig. 3). It should be pointed out that this result cannot be ascribed to an increased cell mortality since the amount of Hsp70 was corrected for LDH release (results are expressed as percentage of Hsp70 releasedpercentage of LDH released). Addition of the three drugs used to perturb protein traffic and secretion (namely monensin, brefeldin A, or tunicamycin) did not significantly affect Hsp70 secretion from resting or heat-shocked Caco-2 cells, a result expected from the above data indicating that these drugs did not elicit any significant up-regulation of Hsp70 (Fig. 3). However, it is important to note that, although these drugs strongly interfere with the classical exocytic pathway, they did not reduce Hsp70 release, demonstrating that Hsp70 is released through a pathway independent of the classical secretory route.
|
Whether Hsp70 release may be related to its association with lipid raft was finally studied. Lipid rafts result from a preferential packing of cholesterol and sphingolipids onto which specific proteins attach. Cholesterol has generally been considered as the major lipid molecule for maintaining raft stability, and together with sphingolipids its concentration is highly enriched in DRMs. Methyl--cyclodextrin was used to disturb raft organization by specifically removing cholesterol (20). We then compared both the association of Hsp70 to DRMs and the release of the protein in the absence and in the presence of methyl-
-cyclodextrin. To confirm the efficiency of methyl-
-cyclodextrin in cholesterol withdrawal, Caco-2 cells were incubated with [3H]cholesterol, and radioactivity was measured before and after methyl-
-cyclodextrin treatment in different fractions. After 24 h of incubation, 73.6 ± 2.5% (n = 6) of [3H]cholesterol was incorporated in Caco-2 cells, and 6 h of incubation with 10 mM methyl-
-cyclodextrin resulted in 58.03 ± 7.8% cholesterol removal from cell lysates. DRMs were then isolated from normal and cholesterol-deficient cell lysates and submitted to immunoblot analysis. Our data revealed that after partial cholesterol removal by methyl-
-cyclodextrin, Hsp70 expression was significantly decreased in supernatants from cholesterol-depleted cells (Fig. 4A) suggesting that released Hsp70 localizes preferentially in lipid rafts whose integrity is required for efficient release of the protein. Accordingly we observed a redistribution of Hsp70 expression from the DRM to the soluble fraction (Fig. 4B).
|
![]() |
DISCUSSION |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
An interesting aspect of the externalization of Hsp70 is the consequence for immune responses. Hsps are among the dominant antigens recognized by the immune system and play an important role in the immune response against cancer (7, 12, 13). Hsp70 released from cells may allow the transfer of antigenic peptides to antigen-presenting cells or may enhance the ability of intact cells to process and present endogenous tumor antigens to specific T cells (24). Consistent with this hypothesis, major histocompatibility complex class II molecules are found to be concentrated in lipid rafts whose integrity appeared to be important to allow efficient antigen presentation in antigen-presenting cells (25). Our data indicate that expression of Hsp70 in DRMs is not restricted to tumor cells since both Caco-2 and the non-tumoral cell line Madin-Darby canine kidney exhibit a similar expression pattern. Indeed raft microdomains appear to be a ubiquitous feature of mammalian cells that likely allow small fractions of Hsps to be transported to and expressed on the cell surface regardless of the cell type. Moreover, on the basis of our findings, we propose that the expression of Hsps in DRMs rather represents a cellular mechanism for controlling signaling events at the cell membrane.
In favor of the latter view, data from Asea et al. (8) suggest that in human monocytes Hsp70 binds specifically to the cell membrane to induce the synthesis of proinflammatory cytokines. The signal transduction cascade stimulated by exogenous Hsp70 results in the activation of nuclear factor-B promoter activity through the lipopolysaccharide receptor CD14 and Toll-like receptor 4. In murine macrophages, Vabulas et al. (9) also define Hsp70 as an exogenous stimulus for the signaling pathway that engages Toll-like receptor 4. However, Hsp70 should be released to exert these functions, and authors from the latter studies (8, 9) do not address this question. Our present data are thus in agreement with previously reported evidence for extracellular functions of Hsp70 and provide new elements that may explain how Hsp70 escapes the intracellular compartment. The presence of Hsp70 in lipid rafts may also allow physical proximity between Hsp70 and CD14 (a glycosylphosphatidylinositol-anchored protein known to be present in lipid rafts), thereby facilitating their interaction as recently suggested (18). Our data also suggest that cell membrane expression and related functions of Hsp70 may not be restricted to innate immune cells (monocytes and macrophages) and may be extended to epithelial cells and to other molecular chaperones such as ER-resident Hsps. In agreement, cell surface expression of molecular chaperones other than Hsp70 (ER-resident in particular) has already been described (5, 26).
In conclusion, here we have generated three lines of evidence suggesting a raft-based mechanism in the targeting of Hsps (Hsp70 in particular) to the extracellular space of epithelial cells: first, the presence of Hsps in DRMs; second, the lack of an inhibitory effect of drugs affecting the classical ER-Golgi secretory route; and third, observations revealing a methyl--cyclodextrin-sensitive extracellular presence of Hsp70. The presence of Hsps in DRMs may have a number of still unexplored implications and is a challenge for future research.
![]() |
FOOTNOTES |
---|
To whom correspondence should be addressed. Tel.: 33-1-40-01-13-34; Fax: 33-1-40-01-13-90; E-mail: marie.bachelet{at}chusa.jussieu.fr.
1 The abbreviations used are: Hsp, heat shock protein; DRM, detergent-resistant microdomain; ER, endoplasmic reticulum; PBS, phosphate-buffered saline; ELISA, enzyme-linked immunosorbent assay; LDH, lactase dehydrogenase.
![]() |
ACKNOWLEDGMENTS |
---|
![]() |
REFERENCES |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|