Rapid Segmental and Subdomain Motions of DNA Polymerase beta *

Soon-Jong KimDagger §, William A. BeardDagger , John Harvey, David D. ShockDagger , Jay R. Knutson, and Samuel H. WilsonDagger ||

From the Dagger  Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709 and the  Laboratory of Biophysical Chemistry, NHLBI, National Institutes of Health, Bethesda, Maryland 20892

Received for publication, August 19, 2002, and in revised form, November 25, 2002

    ABSTRACT
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

DNA polymerase (pol) beta  is a two-domain DNA repair enzyme that undergoes structural transitions upon binding substrates. Crystallographic structures indicate that these transitions include movement of the amino-terminal 8-kDa lyase domain relative to the 31-kDa polymerase domain. Additionally, a polymerase subdomain moves toward the nucleotide-binding pocket after nucleotide binding, resulting in critical contacts between alpha -helix N and the nascent base pair. Kinetic and structural characterization of pol beta  has suggested that these conformational changes participate in stabilizing the ternary enzyme-substrate complex facilitating chemistry. To probe the microenvironment and dynamics of both the lyase domain and alpha -helix N in the polymerase domain, the single native tryptophan (Trp-325) of wild-type enzyme was replaced with alanine, and tryptophan was strategically substituted for residues in the lyase domain (F25W/W325A) or near the end of alpha -helix N (L287W/W325A). Influences of substrate on the fluorescence anisotropy decay of these single tryptophan forms of pol beta  were determined. The results revealed that the segmental motion of alpha -helix N was rapid (~1 ns) and far more rapid than the step that limits chemistry. Binding of Mg2+ and/or gapped DNA did not cause a noticeable change in the rotational correlation time or angular amplitude of tryptophan in alpha -helix N. More important, binding of a correct nucleotide significantly limited the angular range of the nanosecond motion within alpha -helix N. In contrast, the segmental motion of the 8-kDa domain was "frozen" upon DNA binding alone, and this restriction did not increase further upon nucleotide binding. The dynamics of alpha -helix N are discussed from the perspective of the "open" to "closed" conformational change of pol beta  deduced from crystallography, and the results are more generally discussed in the context of reaction cycle-regulated flexibility for proteins acting as molecular motors.

    INTRODUCTION
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INTRODUCTION
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DNA polymerase (pol)1 beta  is a 39-kDa enzyme composed of two distinct domains of 8- and 31-kDa connected by a protease-hypersensitive hinge region. The amino-terminal 8-kDa domain possesses the 5'-deoxyribose phosphate lyase activity required to process the 5'-terminus in a DNA gap during base excision repair. The polymerase active site is part of the carboxyl-terminal 31-kDa domain (for a review see Ref. 1). The domain and subdomain organization of pol beta  is illustrated in Fig. 1, A and B. Although pol beta  appears to have evolved separately from other families of polymerases of known structure (2), it shares many general structural and mechanistic features with other polymerases. The polymerase domain is composed of three functionally distinguishable subdomains. The polymerase catalytic subdomain coordinates two divalent metal cations that assist the nucleotidyl transferase reaction. Two additional subdomains that have primary roles in duplex DNA binding and nascent base pair (nucleoside 5'-triphosphate and templating nucleotide) binding border the catalytic subdomain. These subdomains will be referred to as C (catalytic)-, D (duplex DNA binding)-, and N (nascent base pair binding)-subdomains to discern their intrinsic function. These would correspond to the palm, thumb, and fingers subdomains, respectively, according to the nomenclature that utilizes the architectural analogy to a right hand (3).2

Because of its small size, lack of accessory proteins, and excellent expression properties, pol beta  serves as an excellent model for study of the nucleotidyl transferase reaction, DNA synthesis fidelity, and protein-DNA interactions. Many revealing x-ray crystallographic structures of apoenzyme (4), binary DNA substrate complexes (substrate and product) (5, 6), and ternary substrate complexes (DNA and 2'-deoxynucleoside 5'-triphosphate) (6-8) have been solved.

Comparison of the structure of pol beta  in different liganded states indicates that there are numerous structural transitions that occur upon substrate binding. These transitions include repositioning of the amino-terminal lyase domain toward the carboxyl polymerase domain upon binding DNA. Additionally, a large, ~30°, rotation of alpha -helix M repositions alpha -helix N adjacent to the nascent base pair upon binding a dNTP to form a ternary complex (9). The repositioning of alpha -helix N upon binding a nucleotide results in an "open" (-dNTP) to "closed" (+dNTP) conformational transition and forms an effective active site that coordinates two Mg2+ ions (see Fig. 1C). When bound to single-nucleotide gapped DNA, the overall architecture of pol beta  resembles a doughnut, which suggests that nucleotide access may be restricted (9, 10). Upon binding a correct dNTP and forming a closed complex, extensive contacts are observed between the polymerase and nascent base pair (6, 7). The structure of pol beta  bound to single-nucleotide gapped DNA indicates that the template strand is bent ~90° as it enters the polymerase active site, permitting polymerase stacking interactions with one face of the nascent base pair and the DNA minor groove. This allows for a survey of the geometry of the nascent base pair to probe whether it conforms to Watson-Crick geometry. The structure of a "catalytic intermediate" ternary complex of pol beta  indicates that closure of the N-subdomain can be induced by metal-dNTP binding in the absence of the catalytic metal (8). Kinetic approaches to study nucleotide insertion and fidelity have indicated that rate-limiting conformational changes occur before and after chemistry, suggesting an induced-fit mechanism for nucleotide insertion and fidelity (see Ref. 11). The identity of the proposed conformational changes are unknown, but recent kinetic (8, 10, 12), structural (8), and modeling (11) studies with pol beta  indicate that it is not the large subdomain movements inferred from structural studies. However, the rate and function of this large structural transition remains unknown.

DNA polymerases are more than nucleotidyl transferase catalytic centers. Some DNA polymerases are processive and remain bound to the DNA substrate as they move along the template strand synthesizing DNA. DNA polymerase beta  is known to be processive on short gapped DNA but distributive on non-gapped DNA (13). Because the isolated polymerase domain (31-kDa domain) of pol beta  exhibits only distributive DNA synthesis (14), the 8-kDa lyase domain confers processive DNA synthesis to the intact enzyme. The dynamic description of processive DNA polymerases should include their catalytic properties (e.g. DNA and dNTP binding, conformational changes, and chemistry), as well as their movement (i.e. translocation). The force and velocity of RNA polymerase has been studied as a function of load (15), and it appears to function as a molecular motor. Recently, the vectorial nature of these motors has been modeled in terms of a "Brownian Ratchet" (16-18). The requirements for rectification of Brownian motion seem to be as follows: 1) a cycle of flexibility and/or hinging motion followed by one or more rigid states in the biopolymer, 2) the coupling of nearly irreversible chemical events to selected portions of the cycle, and 3) a change in conformation or binding affinity associated with those events. The myosin-actin and dynein-tubulin systems are prototypes for this model (17, 18). Regulation of flexibility also plays a role in recognition; in particular, some protein-protein associations are mediated by "target-induced folding" of disordered domains (19-21). In those systems, target-dependent structure seems to be a compromise to achieve relatively tight binding without the stringent, and selective, needs of a "lock and key" interface.

We remain cognizant that we are using dynamics measured in hundreds of picoseconds to a few nanoseconds to study cycles requiring at least hundreds of milliseconds; nevertheless, nanosecond flexibility is clearly relevant to an understanding of the conformational events in that cycle. Gangal et al. (22) have shown that the 1-3-ns rotational correlation times of fluorescein conjugated to engineered surface cysteines correlate strongly with the long-term structural disorder reported by crystallographic B-factors for the adjacent backbone elements. The measurement of segmental flexibility has a long history (see Ref. 23); in particular, emission anisotropy has a long history of revealing local motions (24, 25). In addition, time-resolved methods have the advantage of quantifying both the angular extent and the rate of these motions (24, 26, 27). In favorable cases, the fast motion of a segment on a loose "hinge" can be interpreted both in terms of the size of the independent segment and the angular range of facile hinging. Even in flexible coiled polymers, the fast term presents a measure of granularity and offers insight into the persistence of rigid segments (28). In all, nanosecond flexibility appears to be an important predictor of functional flexibility.

Wild-type pol beta  is comprised of 335 amino acids and includes a single tryptophan near the carboxyl terminus (Trp-325; see Fig. 1, A and B). The effects of metal binding on the tryptophan fluorescence and the hydrodynamic shape of wild-type rat pol beta  were characterized previously (29) by time-resolved fluorescence and analytical ultracentrifugation. In this study, we have strategically positioned tryptophan residues in the lyase domain (F25W/W325A) or N-subdomain (L287W/W325A) by site-directed mutagenesis to monitor changes in flexibility of these structural elements upon binding substrates. We interpret changes in flexibility in the context of pol beta  function.

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Materials-- Wild-type and mutant derivatives of human recombinant pol beta  were overexpressed in Escherichia coli and purified as described (30). Oligonucleotide site-directed mutagenesis and activity measurements were performed as described previously (31). The enzyme concentration was determined from the absorbance at 280 nm using an extinction coefficient of 21,200 cm-1 M-1 (32). Acrylamide and melatonin were obtained from Sigma.

A 24-mer DNA substrate was prepared by annealing three high pressure liquid chromatography-purified oligonucleotides (Oligos Etc., Wilsonville, OR) to create a single-nucleotide gap at position 13. Each oligonucleotide was resuspended in 50 mM Tris-HCl, pH 7.4, and 1 mM EDTA, and the concentration was determined from their UV absorbance at 260 nm. The annealing reactions were carried out by incubating a solution of 10 µM primer with 11 µM of both the downstream and template oligonucleotides at 90 °C for 2 min followed by slow cooling to room temperature. The sequence of the gapped DNA substrate was as follows: upstream 12-mer primer, 5'-CCGCTGATGCGC-3'; downstream 11-mer oligonucleotide, 5'-GTCGGTGGGCC-3'; 24-mer template, 5'-GGCCCACCGACAGCGCATCAGCGG-3'. The 3'-end of the upstream primer was synthesized without a 3'-hydroxyl so it would not be extended with the addition of pol beta  and dNTP. The downstream primer was synthesized with a 5'-phosphate.

Absorption and Steady-State Fluorescence-- Absorption spectra were recorded on a Hewlett Packard 8453 UV-VIS spectrophotometer (Palo Alto, CA), and fluorescence emission spectra were obtained on an SLM AB-2 (Thermo Spectronics, Rochester, NY) or SLM8000 (SLM Instruments, Urbana, IL) spectrofluorometer. The protein concentration was between 2.5 and 8 µM, and the sample absorbance at 295 nm was <0.1 with 3-mm path length. Excitation and emission monochromators were set at 4-nm band pass. Emission spectra were collected with excitation polarizer at 54.7° and emission polarizer at 0° to the vertical, and background fluorescence from a solvent blank was subtracted. Steady-state anisotropies were measured using the L-format method with Glan-Thomson calcite prism polarizers. Anisotropy (A) was calculated from the intensity of the vertical (Iv) and horizontal (Ih) measurements with a grating correction factor (G): A = (Iv - GIh)/(Iv + 2GIh). G was calculated from the ratio of intensities (Iv/Ih) when the excitation light was polarized horizontally. Stern-Volmer quenching experiments were carried out as described previously (29). Temperature was controlled at 20 °C with a circulating water bath.

Circular Dichroism-- Circular dichroism spectra were measured using an Applied Photophysics PiStar-180 spectrometer using a 0.1-cm cell thermostated at 20 °C. All measurements were corrected for background signal.

Time-resolved Fluorescence Decay-- Measurements were performed in 50 mM Tris-HCl, pH 7.4, and 0.1 M KCl at 20 °C with a protein concentration of 4.7 to 9.4 µM. To avoid tyrosine absorbance, samples were excited at 295 nm, and the absorbance at 280 nm was 0.1-0.2. The fluorescence intensity profiles were measured by the time-correlated single-photon counting technique as described (28). Time per channel was 85 ps, and 512 channels were recorded. Samples were excited at 295 nm using a synchronously pumped, cavity-dumped, frequency-doubled dye laser as excitation source, with a repetition rate of 4 MHz, a pulse width of 5 ps, and an average UV power under 200 microwatts. The Hamamatsu R2809 MCP photomultiplier operated with a time spread (half-width) of about 90 ps, allowing one to resolve fluorescence lifetimes and/or correlation times as short as 30 ps. Intensity decays were analyzed by sum of two or more exponential functions, as shown in Equation 1.


I(t)=<LIM><OP>∑</OP><LL>1</LL><UL>i</UL></LIM> a<SUB>i</SUB> e<SUP>−t/&tgr;<SUB>i</SUB></SUP> (Eq. 1)
The relative amplitudes (alpha i) and decay constants (tau i) were extracted from the fit. The decay-associated spectra were obtained as described previously (29, 33).

For fluorescence anisotropy decay measurements, protein fluorescence at 340 nm was monitored through a film polarizer oriented parallel IVV(t) and/or perpendicular IVH(t) to the vertical excitation polarization and alternatively recorded. Data were collected up to 20,000 counts in the maximum channel at 20 °C. For each sample, sixteen fluorescence intensity decays were obtained contemporaneously and summed to generate the anisotropy decay, r(t), as follows in Equation 2.
r(t)=<FR><NU>I<SUB>VV</SUB>(t)−GI<SUB>VH</SUB>(t)</NU><DE>I<SUB>VV</SUB>(t)+2GI<SUB>VH</SUB>(t)</DE></FR> (Eq. 2)
The factor G was reduced to unity with a wedge depolarizer (Optics for Research, Caldwell, NJ) by placing the depolarizer at the entrance slit of the monochromator. The reference lamp profile and color shift used for convolution analysis was tested with a monoexponential standard (aqueous solution of melatonin with a fluorescence lifetime of 5.27 ns). The convolution was compared with the experimental fluorescence intensity decay by a nonlinear least-squares (reduced chi-squared; chi 2) minimization. The weighted residuals and autocorrelation function of the weighted residuals were calculated to estimate the reliability of each fit.

The fluorescence data were analyzed by the "sum and difference" method described previously (34). The general procedure was to fit the experimental anisotropy decays to the sum of two exponential functions, shown in Equation 3, where beta fast, beta slow phi fast, and phi slow were the variable parameters.
r(t)=&bgr;<SUB>fast</SUB> e<SUP>−t/&phgr;<SUB>fast</SUB></SUP>+&bgr;<SUB>slow</SUB> e<SUP>−t/&phgr;<SUB>slow</SUB></SUP> (Eq. 3)
The pre-exponential beta  terms directly relate to the amplitude of rotational motion, i.e. are measures of the extent to which the emission is depolarized by each rotational component, thereby measuring the restriction upon diffusion of the residue reporter (35).

    RESULTS
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
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Site-specific Introduction of Tryptophan in pol beta -- In wild-type pol beta , the carboxyl-terminal Trp-325 is located on the surface of the enzyme just after the last helix (alpha O) (Fig. 1B). X-ray structures of rat and human pol beta  indicate that one side of the indole ring is exposed to solvent, and the opposite face makes van der Waals contact with His-285 and Ile-323 (6, 7). Those observations were in excellent agreement with our earlier characterization (29) of the fluorescence properties of Trp-325 in rat pol beta . To probe dynamic aspects of other regions of pol beta , Trp-325 was replaced with alanine, and tryptophan residues were substituted at other strategic positions. In the L287W/W325A double mutant (from this point on, referred to simply as L287W), the tryptophan was introduced near the end of alpha -helix N in the N-subdomain (Fig. 1). Residue 287 was selected for tryptophan substitution because of its solvent-exposed environment and minimal contact with substrates as deduced from crystal structures with and without substrates (6). Crystallographic characterization of pol beta  in various liganded states indicates that numerous structural transitions occur upon binding substrates. The most prominent conformational event occurs upon binding of the correct nucleotide resulting in an open to closed transition where alpha -helix N tilts toward the active site intimately contacting the nascent base pair. The characteristics of L287W thus enabled us to use it as a probe for the effects of substrate binding on the dynamics of alpha -helix N. 


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Fig. 1.   DNA polymerase and DNA substrate organization and structure. A, functional organization and domain/subdomain nomenclature of pol beta . DNA polymerase beta  contributes two enzymatic activities essential during base excision repair. The active sites for these activities (an 8-kDa amino-terminal lyase domain (purple) and the carboxyl-terminal polymerase domain) are found on distinct domains. The polymerase domain is composed of three functionally distinct subdomains referred to as D-, C-, and N-subdomains in reference to their intrinsic function (see text). These subdomains correspond to the thumb, palm, and fingers subdomains for DNA polymerases that utilize the architectural analogy to a right hand (3). The location of the intrinsic tryptophan (Trp-325) and site-specifically engineered tryptophan residues (Trp-25 and Trp-287) are indicated. B, structure of the ternary complex of pol beta  complexed with ddCTP and DNA (blue) (6). The subdomains are colored according to the scheme used in A. C, a comparison of the position of alpha -helixes M and N in the open (-dNTP) and closed (+dNTP) forms of pol beta . Rotation around an axis (alpha -helix M) positions alpha -helix N adjacent to the nascent base pair (blue) to probe whether correct Watson-Crick geometry occurs. A modeled tryptophan at residue 287 is illustrated, and the distance between Calpha of Leu-287 in the open and closed forms is indicated.

For study of the 8-kDa lyase domain, we substituted tryptophan for Phe-25 (i.e. F25W/W325A; from this point on, referred to simply as F25W). The amino-terminal lyase domain possesses lyase, single-stranded DNA binding, and 5'-phosphate binding activities (1). Several of the key residues for these activities have been characterized (36-39). Trp-25 is located on the first helix of the protein (alpha A; see Fig. 1, A and B) and is predicted to be partially solvent-exposed. The crystallographic structure of the pol beta  ternary complex indicates that there are no direct substrate contacts with this residue, and residue 25 is located about 10 Å from the lyase active site (i.e. Lys-72) (6). The structure of the apoenzyme form of rat pol beta  (4), or in complex with non-gapped DNA (7), indicates that the lyase domain is distant from the carboxyl-terminal subdomain (i.e. N-subdomain), in contrast to its position when bound to gapped DNA (6). The open and closed conformations of the amino-terminal lyase domain suggest that this domain is flexible in solution. The dynamic properties of the lyase domain in full-length enzyme are unknown even though the NMR structure of the 8-kDa lyase domain has been solved (37).

Characterization of Polymerase Conformation and Catalytic Activity-- The purity of the recombinant proteins was accessed by SDS-PAGE and found to be greater than 99% (Fig. 2, inset). To access the effect of the mutations on the protein conformation, we measured circular dichroism spectra for wild-type, F25W, and L287W proteins (Fig. 2). The spectra indicate that the mutations had no effect on overall protein folding. The catalytic activity of wild-type enzyme and the mutant derivatives were assessed on a single-nucleotide gapped DNA substrate utilizing Mg2+ as the divalent cation. The single (W325A) or double mutants (F25W/W325A and L287W/W325A) exhibited minimal effects on catalytic activity; kcat for wild-type enzyme was 0.22 ± 0.05 compared with 0.20 ± 0.06, 0.24 ± 0.06, and 0.13 ± 0.02 for W325A, F25W, and L287W, respectively. The DNA binding affinity of the mutant proteins for gapped DNA substrates was also not affected by the mutation as assessed by analytical ultracentrifugation (data not shown).


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Fig. 2.   Circular dichroism spectra for wild-type and mutant pol beta . Spectra were collected at 20 °C in a reaction mixture with 50 mM Tris-HCl, pH 7.4, and 0.1 M KCl and a protein concentration of 10.6 µM. The spectra for wild-type enzyme and the F25W and L287W mutants of pol beta  were nearly identical. The inset shows a photograph of a 12% SDS-PAGE gel. Molecular mass standards are in the left lane (M), and 6 µg of wild-type (lane 1), F25W (lane 2), and L287W (lane 3) pol beta  were loaded on the gel.

Steady-State Fluorescence of Wild-type and Mutant Proteins-- The fluorescence emission spectra for F25W, L287W, and wild-type proteins were measured in the absence and presence of substrates. The emission spectra for F25W, L287W, and wild-type proteins are shown in Fig. 3A. The emission maximum for F25W was ~340 nm, whereas it was 348 nm for the L287W and wild-type proteins indicating solvent-exposed tryptophan microenvironments in these later instances. For F25W, the emission maximum suggests that Trp-25 is less exposed to solvent as compared with other tryptophans. The relative integrated intensities of F25W and L287W were about 2- and 3-fold weaker than that of Trp-325 in wild-type pol beta , respectively. The steady-state anisotropy values for F25W, L287W, and wild-type pol beta  were 0.13 ± 0.01, 0.14 ± 0.003, and 0.16 ± 0.005, respectively, indicating a more flexible tryptophan in F25W and L287W than the wild-type protein. The steady-state parameters for wild-type human pol beta  were similar to those of rat enzyme determined previously (29).


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Fig. 3.   Effect of substrates on the fluorescence emission spectra of wild-type and mutant pol beta . A, the relative fluorescence of wild-type enzyme compared with the F25W and L287W mutants of pol beta . The protein concentration was 2.0 µM. The emission spectra of wild-type (B), F25W (C), and L287W (D) were collected in the presence (dotted lines) of various substrates in 50 mM Tris-HCl, pH 7.4, and 0.1 M KCl. The concentrations of ligands were as follows: +Mg2+, 10 mM; +DNA, 10 mM Mg2+ and a stoichiometric concentration (relative to protein) of gapped DNA; +2× DNA, 10 mM Mg2+ and 2-fold molar excess of gapped DNA; +dTTP, same as +DNA with 50 µM dTTP. The solid line in each panel represents the spectrum in the absence of ligands.

The effects of Mg2+ and DNA binding on steady-state fluorescence were also determined. The binding of 10 mM Mg2+ resulted in a minimal fluorescence decrease for wild-type (2.7%; see Fig. 3B) and L287W proteins (7.8%; see Fig. 3D). In contrast, Mg2+ binding to F25W resulted in a significant decrease of fluorescence intensity (24.8%; see Fig. 3C). The fluorescence quenching detected with F25W suggests metal-induced structural changes around Trp-25. Metal soaking experiments with pol beta  crystals indicated that Mg2+ could bind to the hairpin of the first Helix-hairpin-Helix motif (40). This metal binding motif is also found in the D-subdomain and is proposed to facilitate DNA binding (5). Our data suggest that the Trp-25 probe in the lyase domain senses metal binding, even though it is not proximate to these hairpin residues. Further binding of gapped DNA and dTTP resulted in 25.8% (F25W), 32.3% (L287W), and 29.3% (wild-type) fluorescence decreases as compared with proteins without substrates. Notably, DNA binding resulted in ~7 nm of blue shift in the emission maximum of F25W (Fig. 3C), further indicating that the environment around Trp-25 is altered with DNA binding. Although Phe-25 of wild-type protein is not implicated in DNA binding as deduced from crystallographic structures, its location adjacent to a positively charged groove may indicate a role in DNA binding. The longer DNA molecule used here (24 bp) may interact with a larger binding surface than that observed in crystallographic structures utilizing shorter (16 bp) DNA molecules (6).

Stern-Volmer Quenching of Wild-type and Mutant Proteins-- Effects of substrate binding on the microenvironment of the tryptophan residues were probed by collisional quenching experiments using acrylamide as a quencher (see Table I and Fig. 4). In Fig. 4A, Stern-Volmer plots for F25W, L287W, and wild-type proteins in the absence and presence of Mg2+, single-nucleotide gapped DNA, and dTTP are compared. The data, in the absence of substrates, were linear yielding Stern-Volmer constants (Ksv) of 17.34 ± 0.24 M-1, 20.97 ± 0.57 M-1, and 16.31 ± 0.12 M-1 for F25W, L287W, and wild-type enzymes, respectively. From these results, the relative accessibility of the tryptophans to the quencher was determined to be as follows: wild-type < F25W < L287W. In the presence of substrates (Mg2+, single-nucleotide gapped DNA, and dTTP), Ksv of the respective tryptophans decreased 50.2% (8.64 ± 0.06 M-1), 29.7% (14.74 ± 0.26 M-1), and 14.7% (13.91 ± 0.27 M-1) with F25W, L287W, and wild-type proteins, respectively. The large decrease in Ksv for Trp-25 and the 7-nm blue shift in the emission maximum (Fig. 3C) are consistent with the role of this residue in DNA binding.

                              
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Table I
Acrylamide quenching and fluorescence parameters for wild-type and single tryptophan mutants of pol beta


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Fig. 4.   Acrylamide quenching of tryptophan in pol beta . Measurements were performed at 20 °C in a reaction mixture with 2.0 µM enzyme in 50 mM Tris-HCl, pH 7.4, and 0.1 M KCl. Samples were excited at 296 nm, and emission was recorded at 350 nm. Ligand concentrations were as follows: 10 mM Mg2+, 4 µM gapped DNA, and 50 µM dTTP. A, Stern-Volmer acrylamide quenching of wild-type ( and solid lines), F25W (triangle  and dotted lines), and L287W (open circle  and dashed lines) enzymes in the absence (open) and presence (filled) of ligands. B, Stern-Volmer acrylamide quenching of L287W in the presence (dotted lines) and absence (open circle ; solid line) of various substrates as follows: , +Mg2+; black-square, +Mg2+/dTTP; black-diamond , +Mg2+/gapped DNA; and black-triangle, +Mg2+/gapped DNA/dTTP.

For Trp-287, there was ~30% reduction in Ksv without a change in the emission maximum. The smaller Ksv is consistent with the structural location of residue 287 near the template backbone in the closed liganded ternary complex, whereas it is solvent-exposed in the absence of substrates (open complex). A tryptophan modeled at this position suggests it would be situated 5-6 Å from the template backbone. The relatively small reduction of Ksv for wild-type enzyme is also consistent with the location of its lone tryptophan (Trp-325) on the outer surface of the N-subdomain near the carboxyl terminus. However, the 14.7% decrease of Ksv suggests that substrate binding can influence the accessibility of Trp-325, even though this residue is located on the side of the N-subdomain away from the active site. In the presence of substrates, the relative accessibility of the tryptophans probes is in the order of Trp-25 < Trp-325 < Trp-287. Thus, the most prominent protection from the quenching agent was observed for Trp-25 in the F25W mutant. Even though substrates yielded a decrease in Ksv, the wild-type (Trp-325) and L287W tryptophan environments appear to still be highly accessible to the quencher (Ksv of 13.91 and 14.74 M-1, respectively).

Because of the sensitive location of the tryptophan introduced on alpha -helix N of the N-subdomain (Trp-287) near the templating base, the microenvironment of Trp-287 was studied in the presence of different substrate combinations (see Fig. 4B and Table I). Based on these results, Mg2+ alone, or Mg2+ and dTTP, did not afford significant protection of this tryptophan from acrylamide quenching (Table I). In contrast, binding of the gapped DNA alone resulted in ~21% decrease in accessibility (Ksv = 16.66 M-1) suggesting that most of the protection was coming from DNA itself. The binding of the correct nucleotide, dTTP, had no effect on tryptophan accessibility. This is consistent with ternary complex structures of pol beta  indicating that Leu-287 is exposed to solvent (6, 7). The linearity of the Stern-Volmer plots (Fig. 4), without a hint of curvature, suggests that the quenching mechanism for these proteins is dynamic and that the tryptophan microenvironments are homogeneously accessible.

In Table I, the bimolecular quenching rate constants (kq) were calculated from the mean fluorescence lifetimes (tau m; Table II) and Ksv. The quenching rate constants for the all the proteins appear to be near diffusion-controlled (109-1010 M-1s-1). The relative decreases in kq in the presence of substrates (last column in Table I) are very close to the reductions observed in Ksv indicating that the observed decreases in tryptophan accessibility are primarily because of a decrease in dynamic encounters. The similar quenching parameters for L287W (Ksv and kq) in the presence and absence of the correct nucleotide suggest that the closure of the N-subdomain upon correct nucleotide binding has no effect on acrylamide accessibility for Trp-287 on alpha -helix N. 

                              
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Table II
Fluorescence lifetime decay parameters for wild-type and single tryptophan mutants of pol beta

Time-resolved Fluorescence Decay-- The tryptophan fluorescence lifetime decays of wild-type and mutant proteins were measured in the presence and absence of substrates (Table II). The fluorescence lifetimes of wild-type and L287W can be well represented by the sum of two exponential decays (reduced chi 2 = 1.04-1.30). In the absence of substrates, the lifetimes of Trp-325 (wild-type) were 0.95 and 8.14 ns, and those of Trp-287 were 1.42 and 5.02 ns. These lifetime parameters for the human wild-type enzyme are similar to those of rat pol beta  (1.27 and 8.44 ns) measured previously (29). Thus, despite the 14-amino acid difference between the human and rat enzymes, the microenvironments of Trp-325 from these two sources appear to be similar, and the unusually long lifetime component characteristic of rat pol beta  is also observed with the human enzyme. For the Trp-25 mutant, fits with a two-exponential model showed systematic deviations and high chi 2 values. Three-exponential models fit the F25W decay data adequately (reduced chi 2 = 0.99-1.24).

Binding of substrates to wild-type and mutant proteins had a minimal effect on their decay parameters. For example, there were less than 7 and 5% decreases in the mean lifetimes of F25W and L287W, respectively, in the presence of all substrates (Table II). This is in contrast to the 15 and 36% substrate-induced quenching observed in the steady state for F25W and L287W, respectively (Fig. 3). The wild-type enzyme exhibited the smallest change (<2%) in the mean lifetime induced by substrate binding (Table II). Hence, the steady-state intensity losses are predominantly static. The decay-associated spectra for L287W in the absence of substrates are shown in Fig. 5. The two lifetime components of L287W are in solvent-exposed environments based on the emission maximum (350 nm). The longer lifetime component contributes about 59% to total fluorescence. The decay-associated spectra for L287W in the presence of substrates are similar to those without substrates (not shown) suggesting a similar Trp-287 microenvironment when the N-subdomain is open or closed.


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Fig. 5.   Decay-associated spectra for L287W pol beta . Spectra were determined in 50 mM Tris-HCl, pH 7.4, and 0.1 M KCl at 20 °C in the absence of substrates with excitation at 295 nm.

Time-resolved Fluorescence Anisotropy Decay-- Fluorescence anisotropy decays were collected for the F25W, L287W, and wild-type proteins at 340 nm in the presence and absence of substrates. The fitted parameters (beta fast, phi fast, beta slow, phi slow) and chi 2 values are presented together with initial anisotropy (ro) in Table III. In all cases, the anisotropy decays could be fit best by a sum of two exponentials. Single exponential fits gave more than 5-fold increases in chi 2 for all cases, and addition of a third exponential term led to no significant improvement in chi 2.

                              
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Table III
Time-resolved fluorescence anisotropy decay parameters for wild-type and single tryptophan mutants of pol beta

For wild-type protein, the fast component of Trp-325 has a rotational correlation time of ~0.8 ns contributing 23% to total depolarization. Substrate binding did not alter the fast component (beta fast and phi fast) but increased the slower correlation time (27 and 37 ns in the absence and presence of substrates, respectively; see Table III). This increase in the longer correlation time is consistent with the binding of the single-nucleotide gapped DNA (14.45-kDa). Nucleotide (dTTP) binding did not cause changes in the rotational parameters.

For Trp-25, 30% of the depolarization originated from the fast component (0.78-0.98 ns) in the absence of substrates. In contrast to the significant effects that Mg2+ binding had on the emission spectra (Fig. 3C), metal binding had little effect on the nanosecond mobility of Trp-25 suggesting that this divalent cation only produces static quenching without affecting the dynamics of Trp-25. The longer rotational correlation times are much smaller than those expected for rigid motion of a 39-kDa protein alone (10.76 ns) or complexed with DNA (21.89 ns). It is possible to estimate the rotational correlation time of a rigid hydrated sphere from the Stokes-Einstein equation, shown in Equation 4, where Mw is the molecular mass of F25W, nu  is the calculated partial specific volume (0.739 cm3/g for human pol beta  at 20 °C), h is the degree of hydration (assuming 0.3 cm3/g of protein), eta  is the solution viscosity (~1.002 centipoise at 20 °C), R is the gas constant (8.314 × J M-1 K-1), and T is the absolute temperature (293.15 K).
&phgr;<SUB>calc</SUB>=M<SUB><UP>w</UP></SUB>(&ngr;+h)&eegr;/RT (Eq. 4)
From Equation 4, the predicted rotational correlation time for pol beta  is ~16.3 ns. Further, the asymmetric nature of pol beta  can only yield correlation times that exceed those for a sphere of the same size (29). Hence, the lower phi slow in F25W (11.34 ns) indicates segmental motion of the amino-terminal lyase domain. Most importantly, DNA binding increased phi slow and decreased the depolarization amplitude of the fast component (beta fast) suggesting that both the fast (local) movement of Trp-25 and the 8-kDa lyase domain are restricted in the presence of DNA and are not restricted further upon nucleotide binding.

The anisotropy decay of unliganded Trp-287 can be best described with two rotational correlation times of 0.93 and 21.57 ns (Table III). However, in contrast to tryptophan in wild-type enzyme or F25W, the contribution from the fast component (beta fast) is noticeably higher (44%), indicating a significant angular contribution from the fast component to the total depolarization. The fast correlation time for L287W could be attributed to segmental motion involving several residues in alpha -helix N. Alternatively, it may represent an unusually slow local motion of Trp-287; i.e. the internal rotation of the indole ring about the Calpha -Cbeta and Cbeta -Cgamma bonds corresponding to the chi 1 and chi 2 dihedral angles of the tryptophan side chain (28, 41). The rotational motion of tryptophan side chains in intact proteins typically yield correlation times of 50-400 ps (42-47). Because some of these determinations probably include averaging with slower segmental contributions, we consider 400 ps an upper estimate for this process. Thus, indole side-chain motions are too fast to correspond to a correlation time of 0.93 ns. This correlation time is more reasonably assigned to alpha -helix N tilting in the solitary enzyme.

In the presence of Mg2+ and DNA, the slower correlation time increased to ~33 ns because of DNA binding. Most interesting, further addition of the correct nucleotide, dTTP, resulted in a dramatic decrease in the amplitude of the fast component (44 to 20%) with a minor decrease in the correlation time (0.87 ns). If the short correlation time reflected local side chain rotation only, we would expect the amplitude to remain stable. This is reasonable when we consider that other indicators of the local tryptophan environment indicate that dTTP binding has no effect on fluorescence quenching (Table I), emission maximum (Fig. 3D), and lifetime parameters (Table II). Further, crystallographic structures suggest that Leu-287 would not be affected (solvent exposure) by nucleotide binding (6). The significant reduction of angular freedom induced by correct nucleotide binding can only be observed on the tryptophan located on alpha -helix N (L287W) that interacts with the nascent base pair in the closed conformation. Because the conformational transitions of pol beta  are benchmarked with crystallographic structures (6), our data suggest that the reduced motion of Trp-287 represents rigidity of alpha -helix N in the vicinity of the nascent base pair subsequent to closing of the N-subdomain. In contrast to the results observed for binding the correct nucleotide, the binding of the incorrect nucleotide, dCTP, did not result in a restrained Trp-287 mobility (Table III). This may primarily be because of the fact that DNA polymerases generally bind the incorrect nucleotide with a lower affinity than the correct nucleotide so that the ternary complex with an incorrect nucleotide is not populated significantly. Although the binding affinity for the incorrect nucleotide (dCTP) is increased about 2-fold for the L287W mutant relative to wild-type enzyme, the mutant also binds the incorrect nucleotide with low affinity (Kd = 360 ± 40 nM). To avoid inner filter effects, we are unable to use higher concentrations of the incorrect nucleotide. The dynamics of Trp-287 in binary (DNA) and ternary (DNA, dNTP) pol beta  complexes are illustrated in Fig. 6.


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Fig. 6.   Influence of correct dNTP binding on the segmental motion of alpha -helix N. In the open pol beta -DNA binary complex (left), crystallographic structures indicate that alpha -helix N does not interact with the templating strand (6, 7). The significant reduction in the amplitude of the rapid anisotropy decay of Trp-287 upon nucleotide binding suggests that the angular motion of alpha -helix N is reduced when interactions with the nascent base pair occur (closed complex). The cone angles for the motion of alpha -helix N in the open and closed states are calculated to be 39 and 21°, respectively.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Fluorescence characterization of structure-based site-specific introduction of tryptophan can be an instructive approach in investigating not only its microenvironment but also the segmental dynamics of its structural support. In this study, we have used the wild-type protein with its lone tryptophan (Trp-325) and two engineered tryptophan mutants (F25W and L287W) where Trp-325 had been altered to alanine (W325A) (Fig. 1, A and B). These tryptophan residues were used to probe the effects of substrate binding on the lyase (Trp-25) and polymerase (Trp-287 and Trp-325) domains.

With regard to the amino-terminal lyase domain, Mg2+ significantly quenched Trp-25 (25%; see Fig. 3C). The other tryptophan probes also sensed metal binding to a smaller extent (<8%). Despite the metal-induced steady-state fluorescence quenching observed for Trp-25, metal binding had almost no effect on Trp-25 flexibility (Table III). The 8-kDa lyase domain is connected to the 31-kDa polymerase domain through a protease-sensitive hinge (Fig. 1B). The anisotropy decay parameters for Trp-25 strongly support the conclusion that the lyase domain is highly mobile in solution as evidenced from the much faster rotational correlation time (11 ns) than expected for an ideal sphere (~16 ns). To define the contribution of the lyase domain, we attempted to collect additional data but failed to uncover a single segmental correlation time corresponding to that anticipated for an isolated spherical lyase domain motion (3 ns). We believe that phi slow here includes contributions averaged from both the lyase domain and intact enzyme. In this view, the 8-kDa lyase domain motion in solution probably occurs on a time scale intermediate between 3 and 8 ns.

To probe dynamics within the polymerase domain, we studied the tryptophan in wild-type enzyme (Trp-325) and a tryptophan engineered into alpha -helix N of the N-subdomain (Trp-287). Crystallographic studies indicate that Trp-325 is located on the surface, in a loop after alpha -helix O (Fig. 1B), with one side of the indole ring making van der Waals contact with His-285 and Ile-323 and the other side exposed to solvent (solvent-accessible area = 79 Å2). This is consistent with partial shielding of this tryptophan from acrylamide quenching. Leu-287 is situated near the nascent base pair and is more solvent-exposed than Phe-25 or Trp-325. Modeling tryptophan residues at these positions, by selecting the best tryptophan rotamer from a library, suggests that the solvent-accessible surface of Trp-287 (~160 Å2) would be much greater than Trp-25 (~42 Å2) or Trp-325. Because of the more exposed environment of Trp-287, the indole ring is expected to experience more rapid ring flipping motions as compared with Trp-325. In this case, the rapid anisotropy decay of Trp-287 is far too long (0.93 ns) to correspond to isolated indole ring flipping (<300 ns). Based on Stern-Volmer quenching data, the binding of the correct nucleotide does not influence the accessibility of Trp-287. If the rapid 1-ns component simply represented fast indole ring rotation for this tryptophan, then it would be expected that the amplitude of the short component would remain the same as in the unliganded protein, because the microenvironment of this tryptophan is not influenced by nucleotide binding (see Figs. 2-4 and Tables I-III). The significant reduction in the amplitude of the rapid anisotropy decay of Trp-287 upon nucleotide binding (14 to 22%) indicates an indirect restriction of this tryptophan. An indirect restriction of Trp-287 could originate from segmental motion in alpha -helix N that is constrained upon nucleotide binding. After formation of the closed conformation, alpha -helix N interacts with the nascent base pair, and these additional interactions are expected to limit segmental motion in this helix.

The flexibility of alpha -helix N may play important roles in DNA synthesis. The analysis described above indicates that there is a strong reduction of the amplitude of the alpha -helix N angular motion in the closed conformation after binding a nucleotide. The cone angles for the motion of alpha -helix N in the open and closed states are calculated to be 39 and 21°, respectively (Fig. 6). The larger angular motion of alpha -helix N in the open state may facilitate selection of the correct incoming nucleotide from the pool of structurally similar competing molecules. When a correct nucleotide binds in the pol beta  active site and forms Watson-Crick hydrogen bonds with the templating base, alpha -helix N can probe the geometry of the nascent base pair. Optimum interactions (van der Waals and electrostatic) are expected to occur between the correct nascent nucleotide and alpha -helix N thereby restricting segmental motions of this critical helix. The closed conformation is expected to signal correct geometry to initiate further critical conformational changes and/or chemistry (10, 11). In contrast, if an incorrect nucleotide binds to the pol beta  active site, then the interactions with alpha -helix N and the nascent base pair (templating nucleotide and incorrect incoming nucleotide) are not optimum, and the nucleotide can diffuse rapidly out of the active site before chemistry can occur. An illustration of these events and their postulated relationship to polymerase "selectivity" is presented in Fig. 7.


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Fig. 7.   Relationship between the rapid angular motion of alpha -helix N and DNA synthesis fidelity. The magnitude of the rapid angular motion (dashed arrows) of alpha -helix N in the pol beta -DNA binary complex is significantly larger than that for the ternary substrate complex with a correct incoming nucleotide (Fig. 6). Incorrect nucleotides bind with a lower affinity than correct nucleotides indicating that the dissociation rate constant for the incorrect nucleotide is much more rapid than for the correct. The failure of an incorrect nucleotide to influence the magnitude of the angular motion of alpha -helix N suggests that incorrect nucleotides can rapidly diffuse out of the polymerase active site in the open conformation and that chemistry rarely occurs.

The restricted motion of alpha -helix N observed upon binding a correct nucleotide may also play a role in the motor function of the polymerase to facilitate directional translocation. DNA polymerase translocation (5' right-arrow 3') along the growing DNA duplex during processive DNA synthesis is kinetically assumed to be rapid; i.e. translocation is much more rapid than nucleotide insertion. Additionally, the importance of these motions to correct nucleotide selection could imply that these events are coupled so that translocation would not occur until the correct nucleotide is inserted. The rapid segmental motions observed here are much more rapid than the kinetic step that limits nucleotide insertion, ~3 s-1 (20 °C) (31), indicating that this segmental motion does not represent the rate-limiting step for DNA synthesis (10). Indirect experimental approaches had predicted previously (8, 10-12) that the open to closed structural transition observed with alpha -helix N upon binding a correct nucleotide was too rapid to be rate-limiting for nucleotide insertion.

The anisotropy of Trp-25 also indicate a loss in angular extent or "freezing" of segmental mobility in the lyase 8-kDa domain. Interestingly, unlike L287W, this loss of segmental motion occurs immediately upon DNA binding (Table III) and is not restricted further upon nucleotide binding. Overall, the native Trp-325 is unperturbed by substrate binding, whereas our site-selected reporters (Trp-25 and Trp-287) yielded information about local segmental motions that responded to catalytic cycle-dependent conformational changes, albeit in very different ways. Site-directed reporters like tryptophan and analogs have the potential to detect flexibility changes with functional importance in complexes.

    ACKNOWLEDGEMENT

We are grateful to Jennifer Myers for help with preparation of this manuscript.

    FOOTNOTES

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ On sabbatical leave from the Department of Chemistry, Mokpo National University, Muan, Korea.

|| To whom correspondence should be addressed. Tel.: 919-541-3267; Fax: 919-541-3592; E-mail: wilson5@niehs.nih.gov.

Published, JBC Papers in Press, November 27, 2002, DOI 10.1074/jbc.M208472200

2 The subdomain nomenclature as originally proposed for pol beta  utilized the right-hand analogy (4,48); however, it is functionally opposite to that employed for other DNA polymerases. To eliminate possible confusion, a functionally based nomenclature is employed as outlined in the text.

    ABBREVIATIONS

The abbreviation used is: pol, polymerase.

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

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