The Rate of Peptidyl-tRNA Dissociation from the Ribosome during Minigene Expression Depends on the Nature of the Last Decoding Interaction*

L. Rogelio Cruz-Vera {ddagger}, Elena Hernández-Ramón, Bernardo Pérez-Zamorano and Gabriel Guarneros §

From the Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Apartado Postal 14–740, México Distrito Federal 07000

Received for publication, February 3, 2003 , and in revised form, April 16, 2003.


    ABSTRACT
 TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
The expression of some very short open reading frames (ORFs) in Escherichia coli results in peptidyl-tRNA accumulation that is lethal to cells defective in peptidyl-tRNA hydrolase activity. In an attempt to understand the factors that affect this phenotype, we have surveyed the toxicity of a complete set of two-codon ORFs cloned as minigenes in inducible expression vectors. The minigenes were tested in hydrolase-defective hosts and classified according to their degree of toxicity. In general, minigenes harboring codons belonging to the same box in the standard table of the genetic code mediated similar degrees of toxicity. Moreover, the levels of peptidyl-tRNA accumulation for synonymous minigenes decoded by the same tRNA were comparable. However, two exceptions were observed: (i) expression of minigenes harboring the Arg codons CGA, CGU, and CGC, resulted in the accumulation of different levels of the unique peptidyl-tRNAArg-2 and (ii) the toxicity of minigenes containing CUG and UCU codons, each recognized by two different tRNAs, depended on peptidyl-tRNA accumulation of only one of them. Non-toxic, or partly toxic, minigenes prompted higher accumulation levels of peptidyl-tRNA upon deprivation of active RF1, implying that translation termination occurred efficiently. Our data indicate that the nature of the last decoding tRNA is crucial in the rate of peptidyl-tRNA release from the ribosome.


    INTRODUCTION
 TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
Minigenes are DNA sequences present in bacterial chromosomes that may be expressed into functionally active oligopeptides. In Escherichia coli for example, translation of a peptide encoded in a minigene present in the 23 S rRNA, turns cells erythromycin resistant (1); also, peptides containing five to eight amino acid residues encoded in the attenuator sequence of genes, which confer resistance to chloramphenicol and erythromycin (catA86, cmlA and ermC), inhibit peptidyltransferase activity in bacteria (2).

Translation of two-codon minigenes located in bacteriophage lambda chromosome bar regions is lethal to cells partly defective in peptidyl-tRNA hydrolase activity, but not to wild-type bacteria (3). Translation of bar minigene mRNAs results in premature release of peptidyl-tRNAs from ribosomes (a phenomenon called "drop-off"); under limited Pth1 activity, these peptidyl-tRNAs accumulate in the cell. It has been proposed that lethality stems from the subsequent shortage in the pool of specific tRNAs for further involvement in protein synthesis (4). Recently, evidence that seems to support this inference has been obtained for a ribosome bypassing system (5), but the alternative explanation that peptidyl-tRNAs might be toxic per se has not been ruled out (6).

Translation ends at the termination codon in an mRNA, when the ribosomal peptidyl-transferase presumably hydrolyzes the ester bond between the completed polypeptide chain and the last tRNA. The termination reaction requires the concurrence of the release factors RF-1 or RF-2 (depending on the nature of the termination codon) and other factors catalyzing the release of the mature protein (7, 8). Drop-off is a normal, if relatively rare, event in protein synthesis that can occur during elongation or instead of polypeptide termination (9, 10). If the rates of peptidyl-tRNA synthesis and drop-off exceed the rates of termination and Pth hydrolysis, peptidyl-tRNA accumulates and thus critically reduces the concentration of aminoacylable tRNA and increases that of peptidyl-tRNAs (4, 9). The up-shift to non-permissive temperatures of a thermosensitive pth mutant, pth(Ts), results in peptidyl-tRNA buildup of all the tRNAs assayed. The rates of peptidyl-tRNA accumulation differ as a function of the tRNA species. Thus, families of tRNA cognate to codons for Lys, Thr, and Asn accumulate the fastest, whereas those cognate to codons for Leu, Gly, and Cys accumulate the slowest (11). These results suggest that the drop-off rates depend on the codons involved.

Toxicity and peptidyl-tRNA accumulation in the pth(Ts) mutant is alleviated in strains defective for the translation termination factors RF-3 and RRF (12). Drop-off during minigene mRNA translation is enhanced by these termination factors as well as the elongation factor EF-G (9). In vitro experiments with different synthetic minigenes have shown that the relative rates of termination and drop-off vary according to the composition of the last sense codon, the nature and context of the stop codon, and the length of the mini-ORF and that toxicity is correlated to these conditions (9, 13). In addition, the strong effect of the SD sequence affects peptidyl-tRNA accumulation by driving minigene mRNA through several rounds of translation without dissociation from the ribosome (9). Despite these results, the scarce number and heterogeneity of minigenes studied so far does not allow us to draw conclusions about the role of codon composition in drop-off. We have studied the effect of the last sense codon on peptidyl-tRNA accumulation, minigene mRNA concentration, translation termination, and toxicity to pth-defective cells. Our results indicate that the degree of toxicity stems from the intrinsic inability of certain codon-tRNA complexes to mediate efficient translation termination.


    EXPERIMENTAL PROCEDURES
 TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
Bacterial Strains and Toxicity Assay—The following E. coli K-12 strains were used: C600 (thr1 leu6 thi1 supE44 tonA lacY1), our collection; pth-defective mutants C600rap (C600 pth[rap] zch::Tn10) and C600pth(Ts) (C600 pth[Ts] zch::Tn10) (14); C600rap Tcs, tetracycline-sensitive derivative of C600rap (15); US486 (W3110 prfA1), a thermo-sensitive mutant defective in release factor RF-1 (R. Buckingham collection; Ref. 16); GG05 (US486 pth[rap] zch::Tn10), US486 with bacteriophage P1 (C600rap); and GG06 (C600 pth[rap] prfA1 zch::Tn10), C600rapTcs with P1 (GG05), this work. The viability experiments to estimate the degree of minigene toxicity were performed by streaking a single colony of cells harboring the minigene library on Luria Broth (LB)-agar plates containing 100 µg/ml ampicillin and 1 mM IPTG and incubating at 32 °C for 24 h.

Minigene Library Construction—The complete set of 64 constructs harboring two-codon minigenes was obtained by cloning duplex synthetic oligos into vector pKQV4, which carries the IPTG-inducible tac promoter (Fig. 1A; Ref. 17). An attempt was made to generate the 64 pairs of complementary synthetic oligos, namely 5'-AATTCATGNNNTAAATA-3' and 5'-AGCTTATTTANNNCATG-3' (the EcoRI and HindIII cohesive ends are underlined and in bold, respectively), by random synthesis at positions NNN corresponding to the variable minigene codon (Fig. 1A). Duplexes were obtained by allowing complementary oligos to hybridize in a mixture containing 400 pmol of each random oligo in 100 µl of buffer (10 mM Tris-HCl, pH 7.2, 1 mM EDTA) for 5 min at 95 °C and slowly cooled down to room temperature. Duplexes were then cloned into the vector after their double restriction digestion with both EcoRI and HindIII. Ligation of inserts into this vector was performed using 40 fmol of duplex mixture with 15 fmol of the double-restricted vector in 20 µl of buffer (10 mM Tris-HCl, pH 7.2, 1 mM ATP, 10 mM magnesium acetate, 25 mM NaCl, and 10 units of T4 DNA ligase (Amersham Biosciences)) for 1 h at room temperature. The ligation mix was used to transform C6OOrap cells (18), and selected clones were tested for the absence of the SalI site present in the unsubstituted vector only. Inserts from these clones were sequenced using Big-Dye sequencing kits (Applied Biosystems), using the forward oligo 5'-GACATAACGGTTCTGGC-3', corresponding to a sequence close to Ptac, and the reverse oligo 5'-CTGTTTTATCAGACCGC-3', corresponding to a sequence upstream to the 5 S gene (Fig. 1A). Thirty-two minigenes were obtained after random hybridization, and the rest were obtained by direct cloning of specific duplexes into the expression vector as described above.



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FIG. 1.
Constructs and degree of toxicity of two-codon minigenes. A, schematic diagram of a linear version of the used constructs: each construct contained a single minigene cloned between the EcoRI and HindIII sites (in italics) in pKQV4; the relative positions of other genetic markers are also indicated. Minigenes were under the control of the Ptac/Olac transcriptional promoter and were followed by the transcriptional terminator Trrnb. Translation was controlled by a SD sequence (underlined) and initiation (ATG) and stop (TAA) triplets. B, genetic code indicating the variable codon of the 64 possible two-codon minigene constructs and, for the analyzed cases, the corresponding tRNAs and amino acid residues (columns 2, 3, and 1, respectively, in each box). The viability of C600pth(Ts) and C600rap cells transformed with each minigene was measured after IPTG induction of minigene expression (see "Experimental Procedures"). Different degrees of toxicity are given in different colors: red, minigenes toxic to both pth-deficient strains; blue, partly toxic minigenes that inhibit the growth of pth(rap) cells only; green, non-toxic minigenes. The figures in each box correspond to estimated peptidyl-tRNA fractions, expressed as a percentage of the corresponding tRNA present in either C600pth(Ts) (columns numbered 4) or C600rap (columns numbered 5) after 30 min of expression. Underlined codons are decoded by tRNAs having concentrations comparable with that of tRNAArg-4, typically considered as a scarce tRNA species (22). Codons in half-brackets are cognate to a unique tRNA.

 

Peptidyl-tRNA Levels—Peptidyl-tRNA levels were estimated by a modification of the Northern blot technique of Varshney et al. (19). This method is more specific than the traditional assay (20), because it distinguishes individual tRNAs. Briefly, the transformants for minigene constructs were cultured on LB (100 µg/ml ampicillin) at 32 °C to an A600 value of 0.5 and induced with 1 mM IPTG for different periods of time (see "Results") at 32 °C. To estimate the fraction of peptidyl-tRNA relative to total tRNA, aminoacyl-tRNAs were hydrolyzed by the cupric reaction (21). Total extracted tRNA was resuspended in 20 µl of water and divided into two aliquots: a control sample that was mixed with 90 µl of 20 mM sodium acetate, pH 5.0, and a hydrolysis sample with 10 mM CuSO4. Each reaction was incubated for 30 min at 37 °C and was precipitated with 2 volumes of ethanol after addition of EDTA to a final concentration of 5 mM. The samples were resolved by acid/urea PAGE and transferred to nylon membranes; tRNA-containing species were revealed using specific 32P-labeled oligo probes (5 x 106 cpm/pmol; Refs. 19 and 22). The peptidyl-tRNA fraction was estimated from the radioactivity ratio between the measured radioactivities in the (peptidyl-tRNA/peptidyl-tRNA + tRNA) respective bands as determined in a Typhoon Scan (Amersham Biosciences).

Minigene mRNA Detection—Total RNA was obtained from cultures of transformants for minigene constructs induced with IPTG. Minigene mRNA was revealed by Northern blot analysis using a 32P-labeled DNA probe (2 x 106 cpm/ng; Ref. 23). The 150-bp DNA probe was synthesized by 50 cycles of PCR (95 °C, 30 s; 55 °C, 30 s and 72 °C, 1 min) using 30 fmol of pKQV4 template and 10 pmol of each sequencing oligo (defined above) in a 50-µl reaction mixture containing 40 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 10 mM dithiothreitol, 50 mM NaCl, 50 µCi of [{alpha}-32P]dCTP (6,000 Ci/mmol, Amersham Biosciences), 150 µM concentration each of the other three dNTPs, and 1 unit of Taq DNA polymerase (Applied Biosystems).


    RESULTS
 TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
Minigenes Harboring Codons from the Same Genetic Code Box Show Similar Toxicity—Changes in the last sense codon of some minigenes determine variations in the degree of toxicity for pth mutants (9, 24, 25). To understand the nature of this effect, we used an expression library of two-codon ORF minigenes in which the second, and last, sense codon was any of the 64 possibilities in the genetic code (Fig. 1A). By maintaining the same transcriptional promoter, SD region, initiation and termination codons, and the shortest possible "ORF," we attempted to minimize the number of variables affecting minigene expression and focus on the effect of the different sense codons on toxicity (9, 13). The minigene constructs were expressed in two pth mutants differing in the level of Pth activitiy (pth[Ts] > pth[rap]; Ref. 15), as a means for ranking minigene toxicities. The results, summarized in Fig. 1B, showed a wide variation in the degree of toxicity to the two mutant strains arising from minigene expression and depending on the nature of the second codon: 27 were toxic to both pth(Ts) and pth(rap), 18 were lethal to pth(rap), and 16 had no deleterious effects on either mutant. Unlike the toxic minigenes isolated by Tenson et al. (24), none of our constructs affected wild-type cell growth under the same assay conditions (data not shown). This effect may be due to the lower strength of minigene expression in the vector we used relative to that recorded for the vector used in the previous report (24). Minigenes with any of the three termination codons in the second position do not encode peptides and are therefore expected to be harmless to Pth-defective strains. In effect, the two constructs tested, carrying TAA and TGA respectively, had no toxic effects (data not shown). No correlation was observed between codon pair bias (26) or cognate tRNA scarcity (underlined codons in Fig. 1B; Ref. 22) and minigene toxicity. In general, minigenes harboring codons grouped within the same genetic code box (Fig. 1B) showed a similar degree of toxicity (38 minigenes in 10 out of 16 boxes). Five of these boxes contain codons for two different amino acids (e.g. TTN, Phe/Leu; ATN, Ile/Met-, etc.), suggesting that the codons, rather than the decoded amino acids, determine minigene toxicity. This would not be the case for those minigenes bearing codons within each of the remaining six boxes in which mixed degrees of toxicity were observed (dashed boxes in Fig. 1B).

Non-toxic Minigenes Do Not Mediate Peptidyl-tRNA Accumulation—For a number of minigenes, a direct correlation between toxicity and peptidyl-tRNA concentration has been observed in pth mutants (4, 9, 24). To assess the breadth of this observation, we estimated the relative concentrations of peptidyl-tRNAs for selected minigenes. Initially, we determined the time length of IPTG minigene induction which generated the highest peptidyl-tRNA concentrations. The data showed that, for the toxic (AAT and AAA) and partly toxic (GAT and GAA) minigenes assayed, the highest accumulated fraction was reached in all cases within a 30-min induction period (Fig. 2B). Therefore, all subsequent determinations of peptidyl-tRNA concentration were made at 30 min (Fig. 1B, columns 4 and 5). The relative concentrations of peptidyl-tRNA in C600pth(Ts) varied from 40 to 70% for toxic, 10 to 20% for partly toxic, and 5 and 10% for non-toxic minigenes (Fig. 1B, fourth column in each cell). In C600rap, the relative concentrations of peptidyl-tRNA from toxic and partly toxic minigenes were proportionally higher (Fig. 1B, fifth column in each cell), whereas the concentrations of peptidyl-tRNA for non-toxic minigenes were lower, although similar to those observed for pth(Ts). Wild-type transformants did not accumulate peptidyl-tRNA (data not shown). Except for minigene AAT, encoding Asn, which intriguingly promotes accumulation of peptidyl-tRNALys (Fig. 2A), heterologous peptidyl-tRNAs did not accumulate upon minigene expression: minigene AAA did not accumulate peptidyl-tRNAAsn; minigene GAT did not accumulate peptidyl-tRNAGlu; minigene GAA did not mediate accumulation of peptidyl-tRNAAsp (Fig. 2A); and minigene AGA did not accumulate peptidyl-tRNALys.



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FIG. 2.
Northern blot analysis and time course of peptidyl-tRNA accumulation following expression of toxic and partly toxic minigenes. A, Northen blot of peptidyl-tRNA (pep-tRNA) accumulation at different times after IPTG induction of toxic, AAT and AAA, and partly toxic, GAT and GAA, minigenes in E. coli C600rap. The peptidyl-tRNAs and tRNAs (arrows), treated previously with CuSO4, were revealed by complementary, 32P-labeled, oligos (see "Experimental Procedures"). B, time course graph of percentage of peptidyl-tRNAs accumulated in Northen blots shown in A.

 

Peptidyl-tRNA Accumulation Depends upon the Specific Codon-tRNA Interaction—In six of the genetic code boxes (dashed boxes in Fig. 1B), minigenes showed mixed degrees of toxicity. To understand the basis of these differences, we analyzed peptidyl-tRNA accumulation upon minigene induction in Pth-defective mutants. For example, in the set of minigenes carrying Arg codons CGU, CGC, and CGA, for which tRNAArg-2 is the sole cognate species, the generated peptidyl-tRNAs must be chemically identical. If we assume an equal decoding rate for the mini-ORFs in each of the three minigenes, toxicity would depend on the drop-off rate, because the rate of peptidyl-tRNA hydrolysis by Pth is identical. The relative amounts of peptidyl-tRNAArg-2 accumulated after expression of each minigene in Pth-defective strains ranked in the order CGA>CGT>CGC, as expected from their degree of toxicity (Fig. 3). Accordingly, the peptidyl-tRNA concentrations should reflect the rate of peptidyl-tRNAArg-2 drop-off due to differences in tRNAArg-2 interaction with each of the three codons (see "Discussion").



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FIG. 3.
Northern blot analysis of peptidyl-tRNAArg-2 accumulation and tRNA species cognate to synonymous minigenes. Peptidyl-tRNAArg-2 was determined after 30 min of IPTG minigene induction in C600 (Wt), C600pth(Ts) (Ts), or C600rap (rap) of toxic (CGA), partly toxic (CGT), and non-toxic (CGC) minigenes. tRNA, aminoacyl-tRNA (aa-tRNA) and peptidyl-tRNA (pep-tRNA) were detected as indicated in Fig. 2 with (+) or without (-) treatment with CuSO4 (see "Experimental Procedures"). The percentage peptidyl-tRNA accumulation in the presence of copper is given at the bottom (same figures quoted in corresponding box of Fig. 1B).

 

tRNASer-1 is cognate to the codons in partly toxic minigenes TCT and TCA and clearly accumulates as peptidyl-tRNASer-1 in strain pth(rap) (40%, Fig. 1B); by contrast, accumulation of peptidyl-tRNASer-5, produced by both the partly toxic TCT and the non-toxic TCC minigenes, was not promoted in either strain (Fig. 1B). This suggests strongly that the toxicity of minigenes TCT and TCA is associated with peptidyl-tRNASer-1 accumulation. The data for the minigenes CTA and CTG could be explained in a similar way.

Non-toxic Minigene mRNAs Are Translated—For the expression of different minigene variants, the cellular levels and stability of minigene mRNA correlate with the strength of toxicity. It has been speculated that the increased messenger stability results from a longer interaction period with the ribosome during translation (25). Results with Northern blot assays confirmed that toxic minigenes (e.g. AAA, GTT, and CGA) accumulated high levels of mRNA, whereas non-toxic minigenes (e.g. GCC, GGC, and CGC) did not (data not shown). We then asked whether non-toxic minigene mRNAs were translated at all and, if they were, why their expression did not drive peptidyl-tRNA accumulation. Minigene AGA, lethal to C600pth(Ts), and the non-lethal minigene GGC, were expressed in the presence of antibiotics (Fig. 4). The antibiotics used were pactamycin, which causes ribosome stalling soon after initiation of protein synthesis (27), and erythromycin, which enhances the dissociation of peptidyl-tRNAs containing at least six to eight amino acids (6, 28). As expected, the expression of the lethal minigene AGA resulted in mRNA accumulation even in the absence of the antibiotics. The non-toxic GGC minigene, on the other hand, accumulated mRNA only in the presence of pactamycin. Similar results were obtained with the lethal CGA and the non-toxic CGC minigenes (data not shown). These results indicate that mRNAs of these non-toxic minigenes are translatable; furthermore, in the absence of antibiotics translation termination should have occurred readily, suggesting that the mRNA-ribosome complex may be short lived. Erythromycin did not mediate accumulation of peptidyl-tRNA nor did it stabilize the ribosome-mRNA complex as deduced from the observation that it did not favor mRNA accumulation (Fig. 4). Given that the erythromycin binding site is at the entrance of the 50 S ribosomal subunit tunnel (29), it is not expected to affect dipeptidyl-tRNAs which are too short to reach the site.



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FIG. 4.
Northern blot analysis of mRNAs derived from minigenes with different toxicity levels expressed in the presence of pactamycin and erythromycin. Minigene mRNA accumulation was assayed in C600pth(Ts) cultures 30 min after induction with IPTG. mRNAs (arrow) were revealed by probing total RNA extracted from cultures of bacteria transformed by two minigenes (AGA or GGC), in the presence of 50 µg/ml pactamycin (P) or erythromycin (E) (C, no antibiotic treatment). The DNA probe is described under "Experimental Procedures." The positions of ribosomal RNA bands are also indicated.

 

Efficient Translation Termination Prevents Peptidyl-tRNA Accumulation—To test whether toxic and non-toxic minigenes differ in the efficiency of translation termination, the levels of peptidyl-tRNA, the intermediate previous to termination hydrolysis, were assayed under a condition of defective termination (Fig. 5). An E. coli prfA1-pth(rap) double mutant, defective for both RF1 (thermosensible) and Pth activities, was transformed with constructs carrying minigenes causing different degrees of lethality. In mutant prfA1 the activity of RF1 is greatly reduced at 43 °C (16). We used the partly toxic CGT minigene and the non-toxic variant CGC, for which tRNAArg-2 is the sole cognate isoacceptor. When RF1 was defective, expression at 32 °C of the CGC minigene did not promote detectable peptidyl-tRNAArg-2 accumulation, whereas it did at 43 °C (lane 4 versus lane 8). The partly toxic CGT minigene promoted peptidyl-tRNAArg-2 accumulation at both temperatures (lanes 2 and 6), and accumulation was enhanced at 43 °C. These results suggest that efficient translation termination of non-toxic minigene mRNAs shortens peptidyl-tRNA residence time on the ribosome so that drop-off events remain undetectable. In strain prfA1, which harbors the wild-type pth allele (lanes 10 and 12), no peptidyl-tRNA was identified during toxic minigene expression at 43 °C; this suggests that all the generated peptidyl-tRNA eventually dissociates from the ribosomes and is cleaved by Pth in solution.



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FIG. 5.
Northern blot assays of peptidyl-tRNAArg-2 accumulation during synonymous minigene expression under limiting RF1 and Pth activities. IPTG induction of CGT and CGC minigene expression in E. coli strains US486 [prfA1] and GG06 [pth(rap) prfA1] was done for 30 min at 32 or 43 °C. tRNA species were treated, with (+) or without (-) treatment with CuSO4, and revealed as indicated in Fig. 3.

 


    DISCUSSION
 TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
We used a library of two-codon minigenes to analyze the effect of the variable second codon on Pth-defective cells. The minigenes were ranked as fully toxic, partly toxic, and nontoxic. All analyzed minigenes showed that these categories correlated directly with relative levels of the respective peptidyl-tRNA concentrations. With few exceptions, the four minigenes bearing codons from the same genetic code box showed similar degrees of toxicity (Fig. 1B). This rule held for unmixed boxes, i.e. those encoding only one type of amino acid, as well as for mixed boxes, encoding two amino acids. A nucleotide sequence relationship has been reported for the tRNAs cognate to the codons within the same genetic code box (30, 31). It is, therefore, likely that the chemical nature of the cognate tRNA is an important toxicity determinant. A closer look at the exceptions mentioned above seems to confirm this conclusion.

A long ribosome pause on the last sense codon, arising from inefficient translation termination, eventually results in peptidyl-tRNA dissociation and mRNA stabilization (9, 12). Therefore, toxic minigenes would be defective in translation termination, and non-toxic minigenes would terminate readily. Additional support for this idea comes from two types of evidence: first, mRNA of the non-toxic CGC minigene was stabilized by pactamycin (data not shown), an antibiotic which arrests protein synthesis early after initiation and results in the accumulation of a 40 S·Met-tRNAF·mRNA complex (32); second, the expression of the same CGC minigene promoted the accumulation of normally undetectable peptidyl-tRNA under limited activity of the termination factor RF1 (Fig. 5, lanes 7 and 8; Ref. 16). We were unable to find a direct correlation between the minigene mRNA and peptidyl-tRNA accumulation, i.e. the length of the ribosomal pause and the strength of the codon:anticodon bond. If this strength has an influence on the ribosomal pause, it might be part of a more complex interaction which involves several factors. One factor could be the effect of both the amino acid side chains and the tRNA body of the peptidyl-tRNA on the interaction with the ribosome, in a manner similar to their themodynamic contribution to the binding of the aminoacyl-tRNA and the EF-Tu (33). A second factor affecting ribosomal pausing may be the specific architecture of the codon:anticodon interaction. The Arg codons CGA, CGU, and CGC are decoded by the unique tRNAArg-2 (22). Unlike CGC, CGA is a poor 5' context for amber suppression by the su7-encoded tRNA suggesting that the CGA codon-peptidyl-tRNAArg-2 in the ribosomal P-site can interfere with either the translation of a codon in the A site (34) or with enhanced termination at a stop codon. We showed that minigene CGA clearly promoted peptidyl-tRNA accumulation, whereas minigene CGC did not (Figs. 1B and 3), thus indicating that CGA hinders termination. It has been speculated that the A:I pairing of the CGA codon with the tRNAArg-2 anticodon at the wobble position is structurally distorted, thus hampering decoding (34, 35). Our results, however, showed that synthesis of peptidyl-tRNA occurs readily (Fig. 3), implying that the defect may be at mRNA translation termination. It is possible that wobble position base pair distortion affects translation termination, because minigene CGT, which accumulates intermediate levels of peptidyl-tRNA (Fig. 3), has a less severe distortion at the U:I pair in the CGU-anticodon interaction (34). A similar mechanism could apply for minigenes AGT and AGC, for which tRNASer-3 is the cognate species.

A third factor affecting ribosomal pausing could be the interaction between tRNA in the ribosomal P-site and the release factor in the A-site. For example, in the presence of peptidyl-tRNAGly-3 in the P-site at codons GGA/G, termination efficiency at UAG is higher than in the presence of peptidyl-tRNAGly-2 at the same codons, suggesting an unusual interaction between tRNAGly-2 and RF1 (36). This hypothesis could explain why toxicity and peptidyl-tRNA accumulation for TCT and CTG in the corresponding minigenes is associated to only one of the two cognate tRNAs (Fig. 1B).

What is the distribution of "toxic" and "non-toxic codons" in bacterial genes? Interestingly, AAA/G and AAU/AAC codons in minigenes, which promote high rates of peptidyl-tRNA accumulation (Fig. 2), are frequently located at the beginning of E. coli ORFs. These codons enhance efficiency of translation when substituted at positions two and three of a reporter gene (37, 38). By contrast, codons that promoted low rates of peptidyl-tRNA accumulation are rarely located among the first three positions (e.g. CUN, GCN, and GGN gene code boxes; Refs. 37 and 38). In the pth(ts) mutant, families of tRNAs cognate to these codons are among those with the lowest rates of accumulation of the corresponding peptidyl-tRNAs at non-permissive temperatures (11). The presence of codons prone to drop-off in minigenes may represent an advantage for protein synthesis when located at the initial positions in the mRNA ORFs. These codons might act as sensors of the general availability of charged tRNAs. If the availability is appropriate, elongation proceeds, otherwise abortive drop-off occurs. This strategy would prevent wasteful protein synthesis elongation under limiting tRNA availability.

Interestingly, a high frequency of AAA/G codons, associated to high drop-off rates in minigenes, has been found at the last sense position of the E. coli ORFs where they also promote drop-off (13). This non-random codon distribution at the ends of ORFs has been considered as an important factor in the modulation of translation termination (39). This could be explained, because such codons provide an alternative translation termination mechanism including drop-off and Pth-mediated hydrolysis of the final peptidyl-tRNA.


    FOOTNOTES
 
* This work was supported by Consejo Nacional de Ciencia y Tecnología (CONACyT) Grants 28401N and 37759N (to G. G.) and O28 (ER025) (to Dr. Julio Collado Vives (Universidad Nacional Autónoma de México)) and by the Consejo del Sistema Nacional de Educacién Tecnológica (COSNET). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Back

{ddagger} Supported by a postdoctoral fellowship from COSNET and funds from CONACyT Grant O28. Back

§ During this time was a Université de Paris VII visiting professor and was awarded a sabbatical fellowship by CONACyT. To whom correspondence should be addressed. Tel.: 52-55-747-3338; Fax: 52-55-747-7100; E-mail: guarnero{at}lambda.gene.cinvestav.mx.

1 The abbreviations used are: Pth, peptidyl-tRNA hydrolase; IPTG, isopropyl-1-thio-{beta}-D-galactopyranoside; ORF, open reading frame; oligo, oligodeoxyribonucleotide; SD, Shine-Dalgarno sequence. Back


    ACKNOWLEDGMENTS
 
We thank Marco A. Magos Castro, José Bueno Martínez, and Guadalupe Aguilar González for skilful technical support; Benito Aguilar for obtention of the random minigene constructs; Richard Buckingham for kindly providing strain US486; Jon Gallant for his comments on the manuscript; and Philippe Régnier and associates at The Institut de Biologie Physico-Chimique for the generous hospitality, which made possible writing early versions of this paper.



    REFERENCES
 TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 

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