Identification of the LIM Protein FHL2 as a Coactivator of beta -Catenin*

Yu WeiDagger, Claire-Angélique Renard, Charlotte Labalette, Yuanfei Wu§, Laurence Lévy, Christine Neuveut, Xavier Prieur, Marc Flajolet, Sylvie Prigent, and Marie-Annick Buendia

From the Unité de Recombinaison et Expression Génétique, Institut Pasteur, INSERM U163, 28 rue du Dr. Roux, 75015 Paris, France

Received for publication, July 18, 2002, and in revised form, December 2, 2002

    ABSTRACT
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

beta -Catenin is a key mediator of the Wnt pathway, which plays a critical role in embryogenesis and oncogenesis. As a transcriptional activator, beta -catenin binds the transcription factors, T-cell factor and lymphoid enhancer factor, and regulates gene expression in response to Wnt signaling. Abnormal activation of beta -catenin has been linked to various types of cancer. In a yeast two-hybrid screen, we identified the four and a half of LIM-only protein 2 (FHL2) as a novel beta -catenin-interacting protein. Here we show specific interaction of FHL2 with beta -catenin, which requires the intact structure of FHL2 and armadillo repeats 1-9 of beta -catenin. FHL2 cooperated with beta -catenin to activate T-cell factor/lymphoid enhancer factor-dependent transcription from a synthetic reporter and the cyclin D1 and interleukin-8 promoters in kidney and colon cell lines. In contrast, coexpression of beta -catenin and FHL2 had no synergistic effect on androgen receptor-mediated transcription, whereas each of these two coactivators independently stimulated AR transcriptional activity. Thus, the ability of FHL2 to stimulate the trans-activating function of beta -catenin might be dependent on the promoter context. The detection of increased FHL2 expression in hepatoblastoma, a liver tumor harboring frequent beta -catenin mutations, suggests that FHL2 might enforce beta -catenin transactivation activity in cancer cells. These findings reveal a new function of the LIM coactivator FHL2 in transcriptional activation of Wnt-responsive genes.

    INTRODUCTION
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

beta -Catenin is a binding partner of E-cadherin in cell-cell adherens junctions and a key effector in the Wnt signaling pathway, which plays a critical role in development and homeostasis (1, 2). beta -Catenin is composed of three domains: a regulatory N-terminal region followed by 12 armadillo (arm)1 repeats and a C-terminal transactivation domain. The N-terminal region contains serine and threonine residues whose phosphorylation signals ubiquitin-dependent degradation of cytosolic beta -catenin. Phosphorylation of beta -catenin is controlled by a multiprotein complex composed of tumor suppressor adenomatous polyposis coli, Axin, glycogen synthase kinase-3, and casein kinase Ialpha . The arm repeats in the core region mediate beta -catenin interactions with a majority of partners such as E-cadherin and the transcriptional factors, T-cell factor (TCF) and lymphoid enhancer factor (LEF). Cytosolic accumulation of beta -catenin leads to trans-location of the protein into the nucleus where it forms a complex with DNA-binding factors of the TCF/LEF family (3). This bipartite transcription factor complex recruits multiple transcriptional coactivators and activates TCF/LEF-dependent transcription through the C-terminal trans-activation domain of beta -catenin (4).

The tight regulation of gene expression by Wnt signaling guarantees a stringent spatiotemporal coordination of downstream gene expression in response to developmental and physiological cues. It has been shown that deregulation of beta -catenin, which leads to its nuclear accumulation and activation of gene expression, is implicated in the development of cancer (reviewed in Ref. 5). Among several candidate downstream target genes, beta -catenin activates transcription from the promoters of c-myc, cyclin D1, the matrix metalloproteinase-7, neuronal cell adhesion molecule, and interleukin-8 (IL-8), which are frequently overexpressed in human colon carcinoma (6-11). Different mechanisms by which beta -catenin promotes target gene activation have been proposed. It has been shown that beta -catenin can interact directly with the TATA-binding protein in vitro (12) and with the transcription coactivator CBP/p300, which is able to bind to TATA-binding protein and transcription factor IIB, thus linking beta -catenin to the RNA polymerase II machinery (13-15). Moreover, beta -catenin-interacting proteins, such as the chromatin-remodeling factor Brg-1 and CBP/p300, can be involved in altering chromatin structure to allow access of RNA polymerase II (16). Here, we report the identification of four and a half of LIM-only protein 2 (FHL2) as a novel beta -catenin-binding protein that possesses intrinsic trans-activation activity.

Initially cloned by its abundant expression in human heart and its down-regulated expression in rhabdomyosarcoma cells (17-19), FHL2 (also known as down-regulated in rhabdomyosarcoma LIM protein and skeletal muscle LIM protein 3) belongs to the family of LIM proteins. The LIM domain is a specialized double zinc finger protein motif, and LIM proteins play multiple roles as adapters and functional modifiers in protein interactions (20). Containing exclusively four and a half of LIM domains, FHL proteins display a high degree of homology between family members and tissue-specific expression (21, 22).

The activity of FHL2 in transcriptional regulation has been evidenced in recent reports demonstrating that FHL2 is a coactivator of the androgen receptor (AR) and the cAMP response element-binding protein (CREB) (21, 23). FHL2 activates gene expression through interaction with DNA-binding transcriptional factors (21, 23). A link between FHL2 and beta -catenin was first suggested by the findings that both FHL2 and beta -catenin interact with AR and are capable of enhancing AR function in androgen-dependent transcription (23-26). In this study, we show that FHL2 binds beta -catenin in vitro and in vivo. Although FHL2 alone had no effect on TCF/LEF-dependent transcription, it potentiated the trans-activating activity of beta -catenin on the transcription of the Wnt-responsive cyclin D1 and IL-8 promoters. FHL2 and beta -catenin independently enhanced AR activity in a hormone-dependent manner, but in this context, the combined action of both proteins had only additive effects. Evidence of up-regulated FHL2 expression in primary tumors suggests that FHL2-activating function on beta -catenin may be implicated in oncogenesis by further enhancing the expression of Wnt target genes.

    EXPERIMENTAL PROCEDURES
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Plasmids-- cDNA sequences encoding beta -catenin arm repeats 1-10 (amino acids 132-554) used as the bait in a yeast two-hybrid screen as well as fragments encompassing different arm repeats were inserted in-frame with Gal4 DNA-binding domain (DBD) into the pAS2Delta Delta vector (a gift of Dr. P. Legrain). FHL2 full-length cDNA was isolated from a HeLa cDNA library after a two-hybrid screen with the beta -catenin bait. FHL2 deletion mutants were constructed by inserting PCR-amplified fragments in-frame with the Gal4 activation domain into pACT2 (Clontech). ACT full-length cDNA cloned into pACT2 was provided by Dr. M. Morgan. pGEX-beta -catenin, pGEX-FHL2, and pGEX-FHL2N-term were constructed by inserting full-length beta -catenin and FHL2 cDNAs and FHL2 sequences coding for amino acids 1-126 in-frame with glutathione S-transferase (GST) into pGEX-5X-1 (Amersham Biosciences). pSGHis-beta -catenin and pSGHis-arm1-12 (amino acids 132-702) were constructed by inserting the RGSH6 sequence at the N-terminal end of beta -catenin into pSG5 (Stratagene). Expression vectors for full-length FHL2 (pcDNAFHL2), beta -catenin (pcDNAbeta -cat), and beta -catenin T41A (pcDNAbeta -catT41A) were used in transient transfection assays. The IL-8 promoter-luciferase construct used in this study contains 193 bp upstream of the transcription start site (193-IL-8-Luc) as described previously (11). The promoter with mutated TCF site at position -186 to -177 (193mt-IL-8-Luc) was used as control (11). pTOPFLASH, pFOPFLASH and pDelta NTCF4 were provided by Dr. H. Clevers; pA3Luc (cyclin D1 promoter) was provided by Dr. R. Pestell; pMMTV-Luc was provided by Dr. P. Chambon; and pSG5-hAR (human androgen receptor) was provided by Dr. G. Castoria. Standard recombinant DNA techniques including PCR followed by sequencing were used to construct all of the plasmids.

Yeast Two-hybrid Analysis-- The two-hybrid screen was performed using the mating protocol described by Fromont-Racine et al. (27). CG1945 cells transformed with Gal4 DBD-beta -catenin-arm1-10 were mixed with Y187 cells transformed with a HeLa MATCHMAKER cDNA library (HL4048AH, Clontech). Transformants were selected by their ability to grow on minimal medium lacking tryptophan, leucine, and histidine. Positive clones were then confirmed in beta -galactosidase overlay assay. Prey plasmids with high beta -galactosidase activity were rescued in Escherichia coli, and their sequences were subsequently analyzed. To map interaction domains in beta -catenin and FHL2, deletion constructs were cotransformed into the diploid strain CG1945/Y187 and quantitative beta -galactosidase assay was performed as described previously (28).

In Vitro Binding Assay-- 35S-Labeled proteins were produced in vitro using TNT-coupled reticulocyte lysate system (Promega). The GST-beta -catenin and GST-FHL2 fusion proteins were purified from E. coli according to the manufacturer's instructions (Amersham Biosciences) and then linked to glutathione-Sepharose beads. Incubation with in vitro translated proteins was carried out for 4 h at 4 °C in binding buffer (20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 10% glycerol, 0.05% Triton X-100, and protease inhibitor mixture (Roche Molecular Biochemicals)). Beads were washed with the binding buffer, and bound proteins were eluted with Laemmli buffer at 95 °C and subjected to SDS-PAGE.

Cell Culture, Transfection, and Luciferase Assay-- 293, SW480, CV1 and HeLa cells were maintained in Dulbecco's modified Eagle medium with 10% fetal bovine serum. Transient transfection of 293 cells was carried out by calcium phosphate precipitation with 50 ng of beta -catenin T41A, 0.25-1 µg of FHL2, 250 ng of Delta NTcf4, and 0.5 µg of pTOPFLASH or pFOPFLASH or with 250 ng of beta -catenin T41A, 0.25-1 µg of FHL2, and 0.5 µg of pCyclinD1-Luc or 0.25 µg of pIL-8-Luc. SW480 cell transfection was performed using calcium phosphate precipitation with 0.25 or 1 µg of FHL2, 250 ng of Delta NTcf4, and 0.5 µg of pTOPFLASH or pFOPFLASH. CV1 cells were cotransfected with 0.5 µg of pMMTV-Luc, 50 ng of pSG5-hAR, 0.5 µg of beta -catenin, and 0.25 µg of FHL2 using LipofectAMINE PLUS (Invitrogen). For AR transcriptional activity assays, CV1 cells were washed 3 h after transfection and cultured in Dulbecco's modified Eagle medium supplemented with 10% stripped fetal calf serum in the presence or absence of 10 nM dihydrotestosterone (DHT) (Sigma). The total amount of transfected DNA was kept constant by adding pcDNA3. Each transfection was performed in duplicate and repeated at least three times. A thymidine kinase-beta -galactosidase plasmid was cotransfected to normalize luciferase activity for transfection efficiency. However, because FHL2 was found to activate transcription of this reporter, it could not be used for normalization; the results were confirmed by multiple independent assays.

Antibodies, Coimmunoprecipitation, and Immunofluorescence-- A polyclonal anti-FHL2 antibody was generated by injection of GST-FHL2N-term (amino acids 1-126) into rabbits. Anti-RGSH, anti-beta -catenin, and anti-FLAG antibodies were purchased from Qiagen, Transduction Laboratories, and Sigma, respectively. For coimmunoprecipitation assays, HeLa cells were transfected with His-beta -catenin or His-arm1-12 using LipofectAMINE. Cells were lysed in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5% Nonidet P-40, and protease inhibitor mixture. Cell lysates were incubated with polyclonal anti-FHL2 antibody. Bound proteins were eluted and analyzed by immunoblotting with anti-RGSH monoclonal antibodies at 1:1000 dilution.

Immunofluorescence staining was carried out as described previously (29). HeLa cells grown on coverslips were transfected with 2 µg of beta -catenin T41A and FLAG-FHL2 with calcium phosphate. 24 h later, cells were washed in phosphate-buffered saline, fixed with 3.7% paraformaldehyde, and permeabilized with 0.5% Triton X-100 in phosphate-buffered saline. Cells were then incubated with monoclonal mouse anti-beta -catenin and polyclonal rabbit anti-FLAG antibodies followed by incubation with the corresponding Texas Red- and fluorescein isothiocyanate-coupled secondary antibodies. Images were obtained on a Leica DMRB microscope equipped with a Princeton CoolSnapFX CCD camera controlled by MetaVue software.

RT-PCR Analysis-- Frozen tumor tissues were obtained after surgery from different French hospitals. Total RNA was isolated from tumor and liver tissues using RNA-Plus RNA extraction solution (Quantum Biotechnologies). Up to 2 µg of total RNA was reverse transcribed using Superscript II RT RNase H-reverse transcriptase (Invitrogen) and oligo(dT) primer. PCR was carried out as follows: 94 °C for 3 min followed by 32 cycles at 94 °C for 30 s; 57 °C for 30 s; 72 °C for 1 min, and a final extension of 6 min. PCR products were analyzed in 1.5% agarose gels. The 18 S ribosomal RNA was amplified as control. The primer sequences are as follows: FHL2-F, 5'-GCCAAGAAGTGTGCTGGG-3'; FHL2-R, 5'-GCAACGGGAGGTTACAGAG-3'; 18S-F, 5'-GTAACCCGTTGAACCCCATT-3'; and 18S-R, 5'-CCATCCAATCGGTAGTAGCG-3'.

    RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Interaction of FHL2 with beta -Catenin-- To identify beta -catenin-binding proteins, a yeast two-hybrid system was used to screen a HeLa cDNA expression library with beta -catenin arm repeats 1-10 (amino acids 132-554) as a bait (Fig. 1A). One clone interacting specifically with beta -catenin contained the coding sequence for full-length FHL2 (279 amino acids) (Fig. 1B). The interaction of FHL2 with beta -catenin was further confirmed by GST pull down. Using GST-beta -catenin bound to Sepharose beads and in vitro expressed 35S-labeled FHL2 or conversely with GST-FHL2 and 35S-labeled beta -catenin, we found specific binding of FHL2 to beta -catenin, since GST alone was not able to pull down the binding partners (Fig. 2A).


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Fig. 1.   Interaction of FHL2 with beta -catenin and mapping of the interaction domains by the yeast two-hybrid system. A, schematic representation of beta -catenin and arm repeat constructs used as baits in fusion with GAL4 DBD. beta -Galactosidase activity in yeast cells transformed with beta -catenin arm repeats 1-10 and full-length FHL2 was arbitrarily set to 100. B, schematic representation of FHL2 and the LIM domains used as preys in fusion with GAL4 activation domain to map the interaction domain with beta -catenin arm repeats 1-10.


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Fig. 2.   Interaction of FHL2 with beta -catenin in vitro and in vivo. A, 35S-labeled FHL2 or beta -catenin proteins were incubated with GST-beta -catenin or GST-FHL2, respectively. GST was used as control. 50% of input was loaded on the gels. B, the FHL2 antibody specifically recognizes the N-terminal domain of FHL2. Plasmids expressing LIM domains 3 and 4 (L3-4), full-length FHL2, and the N-terminal LIM domains (L1/2-2) were transfected in HeLa cells. Cells lysates were analyzed by immunoblotting with FHL2 antibody. C, beta -catenin coimmunoprecipitates with FHL2. HeLa cells were transfected with His-tagged beta -catenin and arm repeats 1-12. Cell lysates were immunoprecipitated with FHL2 antibody, and beta -catenin in the immune complexes was revealed by immunoblotting with anti-His antibody (panel I). Expression of beta -catenin (panel II) and FHL2 (panel III) in HeLa cells was revealed by immunoblotting with His and FHL2 antibodies. The arrow in panel I indicates His-tagged arm repeats 1-12 immunoprecipitated by FHL2. IP, immunoprecipitation; IB, immunoblotting.

FHL2 and beta -catenin interaction was tested next by coimmunoprecipitation experiments. We generated a polyclonal antibody against the N-terminal domain of FHL2, which recognized specifically the FHL2 protein (Fig. 2B). His-tagged full-length beta -catenin and His-tagged arm repeats 1-12 were transiently transfected in HeLa cells, and cell lysates were precipitated by FHL2 antibody followed by immunoblotting analysis with anti-His antibody. Both full-length and arm1-12 beta -catenin proteins were revealed in the immune complexes (Fig. 2C, panel I). Specific coimmunoprecipitation of beta -catenin with FHL2 was verified using preimmune sera that failed to precipitate beta -catenin (Fig. 2C, panel IV). Thus, the interaction of FHL2 with beta -catenin observed in the two-hybrid system also occurs in mammalian cells.

To test the binding specificity between beta -catenin and FHL2, we next tested whether ACT, a related member of the FHL family (30), can interact with beta -catenin in the yeast two-hybrid assay. When full-length ACT in the pACT2 vector was cotransformed with the beta -catenin bait arm1-10 into yeast cells, no interaction was observed between beta -catenin and ACT (data not shown).

Mapping of Interaction Domains in FHL2 and beta -Catenin-- Serial deletion mutants of beta -catenin carrying various arm repeat sequences in-frame with GAL4 DBD (Fig. 1A) were first tested for their trans-activation activity in yeast, and constructs devoid of intrinsic activity were used to map the region mediating the binding to FHL2. Stable expression of beta -catenin fragments in yeast was confirmed by immunoblotting with anti-GAL4 antibody (data not shown). Our results show that the arm repeats 3-8, which mediate beta -catenin binding to LEF1 (31), were required but not sufficient for interaction with FHL2 (see Fig. 1A). Deletion of arm repeat 10 resulted in a 4-fold increase of binding activity, designating beta -catenin arm repeats 1-9 as the optimal domain responsible for FHL2 binding.

To map the FHL2 domain mediating the interaction with beta -catenin, overlapping constructs containing different LIM domains in fusion with GAL4 activation domain were tested for interaction with beta -catenin arm repeats 1-10 in the yeast (see Fig. 1B). Although truncated FHL2 proteins were stably expressed in yeast (data not shown), all of the four and half of LIM domains were required for interaction with beta -catenin, consistent with the finding that only full-length FHL2 was pulled out from the initial two-hybrid screen.

FHL2 Stimulates beta -Catenin-activated Transcription from TCF-responsive Promoters-- The beta -catenin-TCF complex activates gene expression in response to Wnt signaling. Therefore, we assessed the effect of FHL2 on beta -catenin trans-activating function in 293 cells using the TOPFLASH luciferase reporter, which contains TCF/LEF consensus-binding sites. As expected, the TOPFLASH reporter gene activity was enhanced by 20-fold by the constitutively active beta -catenin T41A in which threonine 41 was changed to alanine, as frequently found in tumors (Fig. 3A) (32). Importantly, when FHL2 was coexpressed with beta -catenin, the reporter gene activity was further enhanced up to 4.5-fold in a dose-dependent manner (Fig. 3A), indicating synergistic cooperation between FHL2 and beta -catenin on trans-activation of the reporter gene. FHL2 trans-activation activity was dependent on beta -catenin, because expression of the dominant negative TCF4 (Delta NTCF4), which retains DNA binding activity but fails to interact with beta -catenin, completely abolished this effect (Fig. 3A). Furthermore, coexpression of FHL2 and beta -catenin failed to trans-activate the luciferase gene under the control of mutant TCF/LEF-binding elements (FOPFLASH), demonstrating that the synergistic function of FHL2 and beta -catenin was dependent on TCF/LEF. However, FHL2 alone had a weak dose-dependent effect on TOPFLASH and FOPFLASH reporter gene activity (Fig. 3A, lane 10), suggesting that it might also activate transcription through beta -catenin-independent mechanisms.


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Fig. 3.   TCF/LEF-dependent activation of beta -catenin by FHL2. A, luciferase reporter assay with TOPFLASH/FOPFLASH. Plasmid amounts are in micrograms. Luciferase activity in 293 cells transfected with the TOPFLASH reporter and empty vector was arbitrarily determined as 1. B, luciferase assay with TOPFLASH/FOPFLASH reporters in SW480 cells. The ratio of luciferase activity in cells transfected with TOPFLASH versus FOPFLASH reporters is shown. C and D, luciferase assays with a reporter gene under control of the cyclin D1 promoter (C) and the IL-8 promoter (D) in 293 cells. The wild type IL-8 promoter (193-IL8) as well as the promoter with mutated Tcf site (193mt-IL8) was used in the assay. E, subcellular localization of transfected beta -catenin and FHL2 in HeLa cells. Cells cultured on coverslips were transfected with beta -catenin T41A and FLAG-FHL2. 24 h after transfection, cells were immunostained with monoclonal anti-beta -catenin and polyclonal anti-FLAG antibodies followed by Texas Red-conjugated anti-mouse and fluorescein isothiocyanate-conjugated anti-rabbit secondary antibodies.

In SW480 colon carcinoma cells, endogenous wild type beta -catenin is constitutively active because of defective adenomatous polyposis coli in this cell line. Transient expression of FHL2 in SW480 cells resulted in dose-dependent activation of the TOPFLASH reporter, which was inhibited by the dominant negative Tcf4 (Fig. 3B). Thus, FHL2 is able to stimulate the trans-activating activity of both wild type and stabilized mutant beta -catenin.

We then assessed the synergistic function of FHL2 and beta -catenin on natural TCF-responsive promoters. 293 cells were transfected with a luciferase reporter controlled by either the cyclin D1 promoter known to be regulated by the beta -catenin-TCF complex (8) or the IL-8 promoter, recently identified as a direct beta -catenin-TCF target (11). As found for the synthetic TOPFLASH reporter, FHL2 increased the cyclin D1 promoter activity in association with beta -catenin in a dose-dependent manner (Fig. 3C). This cooperative effect was strongly inhibited by Delta NTcf4 and totally abolished in reporter assays using a cyclin D1 promoter carrying mutated TCF-binding sites (data not shown). Similarly, coexpression of beta -catenin and FHL2 synergistically activated the wild type IL-8 promoter, and this effect was abolished when the TCF-binding site in the promoter was mutated (Fig. 3D). These data suggest that the synergistic interaction of FHL2 and beta -catenin might function in vivo on Wnt-responsive genes.

We next examined cellular localization of beta -catenin and FHL2 in transfected cells by immunofluorescence analysis. When constitutively active beta -catenin T41A and FLAG-tagged FHL2 were exogenously expressed in HeLa cells, the two proteins showed a predominant nuclear staining (Fig. 3E), suggesting that their interaction may occur in the nucleus.

Increased FHL2 Expression in Liver Tumors-- To assess the implication of FHL2 in activated expression of cancer-related Wnt-responsive genes, we examined its expression in hepatoblastoma in which a high rate of genetic mutations is associated with nuclear accumulation of beta -catenin in a majority of cases (32, 33). FHL2 expression was detected by RT-PCR in all of the tumor samples (Fig. 4). Importantly, in 8 of 10 cases, FHL2 expression was markedly up-regulated in tumors compared with matched nontumor livers. This finding suggests that FHL2 expression might play a role in tumor cells by enhancing the trans-activation function of beta -catenin.


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Fig. 4.   Enhanced FHL2 expression in hepatoblastoma. FHL2 expression was analyzed by RT-PCR in tumors (T) and matched nontumor tissues (N). 18 S rRNA was amplified as control.

Enhancement of AR Transcriptional Activity by FHL2 and beta -Catenin-- It has been shown that FHL2 as well as beta -catenin can individually interact with AR and activate AR-driven transcription (23, 24). The binding of FHL2 to AR was confirmed by GST pull-down experiments using GST-FHL2 and in vitro translated 35S-labeled AR (Fig. 5A). To explore the potential effect of the interaction between FHL2 and beta -catenin coactivators on AR function, we cotransfected CV1 cells with a luciferase reporter gene under control of the mouse mammary tumor virus promoter (MMTV-Luc) known to be regulated by steroid hormone receptors and vectors expressing wild type beta -catenin, FHL2, and human AR. In accordance with previous reports, luciferase activity was enhanced ~2-fold by either beta -catenin or FHL2 in the presence of DHT (Fig. 5B). When beta -catenin and FHL2 were coexpressed with AR, they enhanced AR transcriptional activity by ~4-fold (Fig. 5B), indicating that the combined effect of both proteins was only additive. This effect was dependent on the presence of DHT, indicating that transcriptional activation of AR by beta -catenin and FHL2 was not attributed to nonspecific interactions of beta -catenin or FHL2 with the reporter. This result was further confirmed by the observation that beta -catenin and FHL2 were not able to activate the MMTV promoter in the absence of AR (Fig. 5B).


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Fig. 5.   Activation of AR transcriptional activity by FHL2 and beta -catenin. A, in vitro binding assay using 35S-labeled AR with GST-FHL2 or GST. 50% of input was loaded on the gel. B, pMMTV-Luc reporter assay. Luciferase activity was measured in CV1 cells transfected with the indicated expression vectors in the presence or absence of DHT.


    DISCUSSION
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The association of beta -catenin with TCF is an essential step in the transduction of the Wnt signal, and transcriptional activity of the beta -catenin-TCF complex can be modulated by coactivators and corepressors that interact with beta -catenin or TCF. Here we report that FHL2 is a novel partner and coactivator of beta -catenin. The physical interaction between FHL2 and beta -catenin was demonstrated by in vitro and in vivo assays, including yeast two-hybrid screens, in vitro pull-down assays, and coimmunoprecipitation. Further characterization of the interaction using the yeast two-hybrid assay showed that beta -catenin arm repeats 1-9 and the complete set of LIM domains in FHL2 were required for optimal binding. By contrast, no interaction could be detected between beta -catenin and ACT, a LIM domain protein closely related to FHL2, ruling out the possibility that LIM domains might mediate nonspecific association with beta -catenin. Furthermore, we show that FHL2 potentiates beta -catenin trans-activating function on TCF/LEF-dependent transcription of Wnt-responsive genes such as cyclin D1 and interleukin-8 in human kidney and colon cells. Importantly, FHL2 expression in the absence of nuclear beta -catenin did not affect the activity of TCF target gene promoters, and cooperation between FHL2 and beta -catenin was strictly dependent upon the presence of consensus TCF-binding sites in the cyclin D1 and IL-8 promoters. Finally, ectopically expressed FHL2 and beta -catenin were found to colocalize in HeLa cell nuclei, suggesting that nuclear interaction of these proteins might be involved in enhancing TCF transcription.

Taken together, our data support the idea that FHL2 might be recruited by beta -catenin to TCF-dependent promoters, although no direct evidence has been provided so far for the interaction of FHL2 at an endogenous beta -catenin target promoters. While this paper was in revision, similar conclusions were reported by Martin et al. (34) who demonstrated that FHL2 specifically and functionally interacts with endogenous beta -catenin in vivo but not with LEF-1 and that beta -catenin is able to bind simultaneously FHL2 and LEF-1, forming a ternary protein complex in vitro. The authors identified the N-terminal region and first arm repeat of beta -catenin as the FHL2-binding region. However, in our study, the arm repeats 1 and 2 of beta -catenin were required for the interaction with FHL2, but optimal binding efficiency was observed for repeats 1-9. This apparent discrepancy might be explained by different strategies that have been used to localize the interacting regions. Alternatively, it is conceivable that different beta -catenin domains might be able to bind FHL2. It is striking that different regions in beta -catenin have been implicated in the binding of several partners, including TATA-binding protein (12), CBP/p300 (13-15, 35), and adenomatous polyposis coli (36, 37).

In previous studies, FHL2 has been shown to bind different transcription factors acting either as a transcriptional coactivator or as a corepressor (21, 23, 38). Interestingly, FHL2 has been shown to activate beta -catenin-dependent transcription in epithelial cells (this study and Ref. 34) while it has opposite down-regulating effect in myoblasts, suggesting a cell type-specific regulation of beta -catenin function by FHL2 (34). LIM domains function as molecular adapters mediating the assembly of multiprotein complexes. Therefore, FHL2 might target other cofactors to beta -catenin to link the beta -catenin-TCF complex to the RNA polymerase II machinery or to chromatin-remodeling complexes.

The impact of FHL2-beta -catenin interaction on AR transcriptional activity remains unclear, since we did not observe a cooperative effect of these AR coactivators. Furthermore, all of the FHL2 deletion mutants analyzed had lost the ability to bind AR as well as beta -catenin, and the activity of FHL2 and beta -catenin on AR transcriptional function did not appear to be dose-dependent.2 Therefore, our data might be interpreted as additive effects of each individual protein in contrast with the results obtained for TCF/LEF, which indicates that synergistic activity of beta -catenin and FHL2 may be dependent on the promoter context. Further studies using FHL2 null cells or FHL2 mutants retaining only binding to either AR or beta -catenin would shed light on the role of FHL2-beta -catenin interaction on AR function.

Whether the interaction of FHL2 with beta -catenin plays a role during oncogenesis is an interesting issue. In human cancers affecting epithelial tissues, beta -catenin is frequently activated and the stimulating function of FHL2 on beta -catenin might have impact on oncogenic processes. This notion is strongly supported by our finding that the cyclin D1 promoter is markedly activated by FHL2 in a beta -catenin-dependent manner and that FHL2 expression is up-regulated in hepatoblastoma. In this pediatric liver tumor, beta -catenin was found to be mutated in >50% of cases (32, 33), and overexpression of cyclin D1 was correlated with beta -catenin mutation (39). In recent studies of prostate cancer (40), nuclear expression of FHL2 has been detected at higher levels in tumor cells than in normal prostate epithelium, and nuclear trans-location and transcriptional activity of FHL2 might be induced by Rho signaling. Rho family members are frequently overexpressed in human tumors, and the activation of Rho signaling might be involved in the migration and dissemination of tumor cells. Localized in both cytoplasm and nucleus, FHL2 has been found to bind multiple partners including alpha - and beta -integrins that are key regulators of cell adhesion and migration (22). Thus, functional interactions between FHL2 and beta -catenin might have important consequences in multiples aspects of the activities of both proteins.

    ACKNOWLEDGEMENTS

We thank Dr. M. Fabre for providing tumor tissues, Drs. C. Transy and A. Reimann for insightful discussion, and Drs. F. Bergametti and O. Bischof for critical comments on the paper. We are grateful to Prof. P. Tiollais for constant interest in this work. We also thank Drs. R. Pestell, H. Clevers, P. Chambon, M. Morgan, P. Legrain, and G. Castoria for kindly providing constructs used in this study.

    FOOTNOTES

* This work was supported in part by Grant 4395 from the Association pour la Recherche contre le Cancer (ARC).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed. Tel.: 33-1-40-61-33-07; Fax: 33-1-45-68-89-43; E-mail: ywei@pasteur.fr.

§ Supported by the Fondation pour la Recherche Médicale.

Supported by the ARC.

Published, JBC Papers in Press, December 3, 2002, DOI 10.1074/jbc.M207216200

2 C. Labalette and Y. Wei, unpublished data.

    ABBREVIATIONS

The abbreviations used are: arm, armadillo; IL, interleukin; FHL2, four and a half of LIM-only protein 2; ACT, activator of CREM in testis; AR, androgen receptor; TCF, T-cell factor; LEF, lymphoid enhancer factor; CREB, cAMP response element-binding protein; CBP, CREB-binding protein; DHT, dihydrotestosterone; His, histidine epitope tag; RT, reverse transcription; MMTV, mouse mammary tumor virus; DBD, DNA-binding domain; GST, glutathione S-transferase; Luc, luciferase.

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
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