From the
Food Science and Human Nutrition Department and the
Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611 and the ¶Plant Biology Department, Michigan State University, East Lansing, Michigan 48824
Received for publication, March 21, 2003 , and in revised form, March 28, 2003.
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ABSTRACT |
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INTRODUCTION |
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The folate molecule is tripartite, comprising pteridine, glutamate, and p-aminobenzoate (pABA)1 moieties (Fig. 1A). In plants, biochemical and genomic data indicate that the pteridine moiety is made in the cytosol, that the pABA moiety is made in the chloroplast, and that the two are coupled together and glutamylated in the mitochondrion (Fig. 1B) (reviewed in Refs. 5 and 6). In the course of folate synthesis, pABA must therefore exit chloroplasts, cross the cytosol, and enter mitochondria. This elaborate trajectory is not seen in bacteria and fungi, in which the entire pathway is cytosolic (7, 8).
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Recent reviews of plant folate synthesis have tacitly assumed that pABA exists as the free acid in plant cells and that it moves between organelles in this form (5, 6). This assumption is questionable because there is no information about the endogenous level of free pABA in plants, but there are various reports of pABA conjugates. Thus, the -D-glucopyranosyl ester of pABA (pABA-Glc) and N-acetyl and N-formyl pABA have been found as metabolites of supplied pABA in cell cultures of Eucalyptus and Solanum spp (9, 10, 11), and triterpenoid pABA esters have been identified in cucurbit seeds (12). The N-glucoside of pABA-Glc has been reported from plant cell cultures (9) but is probably a chemical artifact. Arylamines such as pABA and pABA-Glc readily condense with glucose and other aldoses (13), and this reaction can occur in culture media (14) and during alcohol extraction (15).
The possible presence of pABA esters or other conjugates raises two questions relating to the folate synthesis pathway and its engineering. First, are such derivatives the major form of pABA in plants? This is so for the pABA analog p-hydroxybenzoate, of which >98% accumulates as derivatives (16). Were this true of pABA, it would suggest that pABA derivatives could be reclaimable storage pools or substrates for membrane transport. Second, are the contents of pABA and its derivatives large as compared with the pABA demand for folate synthesis? If not, enhancing the capacity for pABA synthesis would need to be included in engineering strategies to raise plant folate levels.
To answer these questions, we used radiotracer methods to investigate pABA conjugate formation and developed HPLC procedures to quantify free and conjugated pABA. Having identified pABA-Glc as an important conjugate, we investigated the enzyme activities responsible for its formation and reconversion to pABA and showed that these activities are located in the cytosol. We focused particularly on tomato fruit and pea leaves because folate synthesis has been studied in both systems and because tomatoes are our choice for engineering (5, 17).
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EXPERIMENTAL PROCEDURES |
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Plants and Growth ConditionsTomato (Lycopersicon esculentum cv. Micro-Tom), melon (Cucumis melo), and cotton (Gossypium hirsutum) were grown in potting soil in a chamber (16-h days, photosynthetic photon flux density 200 µmol of photons m2 s1, 23 °C/8-h nights, 20 °C). Pea (Pisum sativum cv. Laxton's Progress 9 or Little Marvel) was grown in vermiculite at 20 °C in 12-h days (200 µmol of photons m2 s1) for 10 days. Arabidopsis thaliana (ecotype Columbia) was grown in potting soil at 2330 °C in 12-h days (200 µmol of photons m2 s1). Maize (Zea mays) and bean (Phaseolus vulgaris) were greenhouse-grown. Lettuce (Lactuca sativa) and spinach (Spinacia oleracea) were purchased locally.
[14C]pABA Metabolism by Plant TissuesDisks (1-cm diameter) or squares (1 cm2) were cut from all tissues except Arabidopsis leaves, which were used whole. The surface of the midrib was shaved from the abaxial surface of leaf tissues, and pericarp disks were given eight shallow radial cuts on the inner face. [14C]pABA dissolved in 310 µl of water was applied to the cut surfaces. Tissues were incubated in Petri dishes for 6 h at 24 °C in light (200 µmol of photons m2 s1), and then shaken (100 rpm) for 15 min in 5 ml of 0.1 mM pABA to remove non-absorbed label, which was quantified by scintillation counting. Tissues were extracted by grinding in 2 ml of semifrozen methanol. Aliquots (60 µl) of the methanol extract were separated by TLC on Silica Gel 60 F254 plates developed with ethyl acetate:methanol:water (solvent A, 77:13:10, v/v/v). Radioactive zones were detected by autoradiography and scraped from the plate for scintillation counting. pABA and its metabolites were visualized in UV light
[14C]pABA Metabolism by Yeast and Escherichia coliSaccharomyces cerevisiae strain 971/6c (Mata ade2-1 his3-11,15 leu2-3,112 ura3-1 trp12 can1) (18) was cultured at 30 °C in minimal medium with appropriate supplements as described (17). E. coli strain K12 was cultured at 37 °C in minimal medium (19). Exponentially growing 40-ml cultures received 3.7 µCi (yeast) or 1.8 µCi (E. coli) of [14C]pABA (55 mCi mmol1) and were incubated until 28% (yeast) or 46% (E. coli) of the label had been taken up. Cells were then pelleted, extracted in methanol, and analyzed by TLC as above.
Preparation and Quantification of pABA-GlcBatches (5 g) of 1-cm disks cut from mature green tomato pericarp were supplied as above with unlabeled pABA (K+ salt, 1 µmol/disk) for 16 h, frozen in liquid N2, and pulverized. The ground material was extracted successively with 25 and 10 ml of methanol. After centrifuging to clear, the pooled extracts were concentrated in vacuo at 28 °C to 1 ml, and 0.5-ml samples were fractionated on a silica gel column (70230 mesh, 2.5 x 18 cm) equilibrated in and developed with solvent A. Fractions containing pABA-Glc were identified by TLC, pooled, dried in vacuo, and redissolved in 2 ml of methanol; overall pABA-Glc yield was 51%. NMR analyses were carried out on this sample. pABA-Glc was further purified by HPLC on a 5-µm Discovery C18 column (250 x 4.6 mm, Supelco) eluted with methanol:water (70:30, v/v) at a flow rate of 1 ml min1. Peaks were detected by absorbance at 295 nm and by fluorescence (290-nm excitation, 340-nm emission). pABA-Glc was quantified spectrophotometrically either directly or as free pABA after hydrolysis in 0.1 M HCl at 80 °C for 3 h, drying in vacuo, and redissolving in 0.1 M potassium phosphate, pH 7.2. The molar extinction coefficient (
) for pABA was taken as 15,000 at 267 nm (20). An
value of 18,120 at 296 nm (in 0.1 M potassium phosphate, pH 7.2) was calculated for pABA-Glc from hydrolysis data.
NMR and LC-MS AnalysesFor NMR analysis, the sample was dried under nitrogen and redissolved in 0.5 ml of CD3OD. One-dimensional 1H NMR spectra were acquired at 500 MHz on a Varian Unity spectrometer (Palo Alto, CA) using a 5-mm probe. Acquisition parameters were such as to give close to fully relaxed spectra for integration of signal intensities (90o pulses, 15-s recycle time). LC-MS analysis was conducted using a Thermo Finnigan TSQ 7000 triple quadrupole mass spectrometer with APCI interface in selective reactions monitoring MS2 mode. N2 pressure was 80 p.s.i. for the sheath and 10 p.s.i. auxiliary. The vaporizer was held at 500 °C, and the heated capillary was held at 200 °C. The corona current was set to 3 kA, maintaining a voltage of 4 kV. Argon was used as a collision cell gas at a pressure of
2.5 mTorr and a collision energy of 10 V. The 5-µm Synergi Polar-RP column (50 x 4.6 mm, Phenomenex) was eluted with 5 mM ammonium formate, 0.1% (v/v) acetic acid using a linear methanol gradient (285% in 5 min) at a flow rate of 0.5 ml min1.
Estimation of pABA and FolatesFor pABA analyses, tissue (0.5 g of fresh weight) was ground in 5 ml of semifrozen methanol; the supernatant was removed, and the residue was re-extracted by shaking with another 2 ml of methanol for 5 min at 22 °C. Two samples of the pooled extracts were dried in vacuo; one (for free pABA estimation) was dissolved in 2 ml of water, and the other (for conjugated pABA estimation) was dissolved in 2 ml of 0.1 M HCl and heated at 80 °C for 23 h. Tests with [14C]pABA-Glc, N-glucosyl [14C]pABA, and N-(p-aminobenzoyl)-L-glutamate established that these conditions give essentially full hydrolysis of pABA-Glc or N-glucosyl pABA but negligible (2%) cleavage of the amide bond between pABA and glutamate and so do not release significant pABA from folates. The extracts were then applied to 1-ml AG 50 (H+) columns, which were washed with 15 ml of water (for free pABA) or 5 ml of 1 M HCl followed by 15 ml of water (for conjugated pABA). The columns were eluted with 5 ml of 1 M NH4OH, and the eluates were lyophilized, redissolved in 0.2 ml of 0.1 M sodium citrate, pH 3.7, and partitioned against 0.6 ml of ethyl acetate. pABA was recovered from the organic phase by back extraction with 0.18 ml of 50 mM NaOH, which was neutralized with acetic acid. Samples of the neutralized extract were injected onto a 5-µm Discovery C18 column (250 x 4.6 mm, Supelco) and eluted with 0.5% acetic acid:methanol (80:20, v/v) at a flow rate of 1 ml min1. Peaks were detected by fluorescence (290-nm excitation, 340-nm emission). The pABA peak was quantified relative to standards. Data were corrected for the recovery of pABA spikes added to plant samples at the start of extraction; recoveries were 2864% and 3744% for unhydrolyzed and hydrolyzed samples, respectively. Free pABA values were further corrected for the proportion of pABA-Glc spikes (1129%) that broke down to pABA during processing. Tests with [14C]pABA-Glc showed that
10% breakdown occurred during the AG 50 step alone. Similar tests with N-glucosyl [14C]pABA indicated
30% breakdown during this step. Folates in Arabidopsis leaves were extracted according to Ref. 21 and then isolated by affinity chromatography, conjugase-treated, and determined by HPLC essentially as described (22). The folates were quantified using fluorescence detection at 295 and 356 nm for excitation and emission, respectively (23).
Subcellular FractionationPea (cv. Laxton's Progress 9) mitochondria and chloroplasts were isolated and purified on Percoll gradients as described (24, 25), resuspended in 25 mM Tris-HCl, pH 7.5, containing 10 mM -mercaptoethanol (buffer A), and broken by 45 cycles of freezing and thawing. Organellar extracts were cleared by centrifugation (15 min, 16,000 x g), and glycerol was added to give a final concentration of 10% (v/v). To prepare a cytosol-enriched fraction, 15 g of pea leaves were homogenized in a Waring blender in 100 ml of potassium phosphate buffer, pH 7.2, containing 0.33 M sorbitol, 5 mM sodium ascorbate, 0.1% polyvinylpyrrolidone-40, and 10 mM
-mercaptoethanol, using three 3-s pulses at setting 3. Chloroplasts and mitochondria were pelleted by centrifugation (3 min at 500 x g and 20 min at 18,000 x g, respectively), and the resulting supernatant was desalted on a PD-10 column (Amersham Biosciences) equilibrated in buffer A containing 10% glycerol. The chloroplast stromal marker NADP-linked glyceraldehyde-3-phosphate dehydrogenase, the mitochondrial matrix marker fumarase, and the cytosol marker 5,10-methylenetetrahydrofolate reductase were assayed as described (26, 27). Fumarase was inactivated by freezing and thus was assayed in fresh samples. Other activities were measured after freezing in liquid N2 and storing at 80 °C.
Assay of pABA Glucosyltransferase and pABA-Glc EsteraseActivities were measured at 30 °C; product formation was essentially linear with time. pABA glucosyltransferase was assayed in the forward (pABA-Glc synthesis) direction in 10- or 20-µl reaction mixtures containing 20 mM Tris-HCl, pH 7.5, 8 mM -mercaptoethanol, 4 mM UDP-glucose, and the indicated amounts of [14C]pABA and glycerol. After 13 h, [14C]pABA-Glc was quantified by TLC as above using 5-µl aliquots of reaction mixtures. Alternatively, assay mixtures were applied to 1-ml columns of AG 4-X8 (OH) from which [14C]pABA-Glc was eluted with water (5 ml) and counted. pABA-Glc esterase was assayed in 5-µl reaction mixtures containing 25 mM Tris-HCl, pH 7.5, 10 mM
-mercaptoethanol, and 20 nCi (0.36 nmol) of [14C]pABA-Glc. [14C]pABA release was quantified after 13 h by TLC. Alternatively, assay mixtures were applied to 1-ml columns of AG 4-X8 (OH); after washing with 20 ml of water, [14C]pABA was eluted with 5 ml of 1 N HCl and counted. Reverse-direction pABA glucosyltransferase assays were the same except that they contained 20 mM UDP.
Partial Purification of pABA Glucosyltransferase from Pea Leaves Pea (cv. Laxton's Progress 9) leaves (20 g) were pulverized in liquid N2 and extracted in 60 ml of buffer containing 50 mM Tris-HCl, pH 8.0, 10 mM -mercaptoethanol, 10 mM sodium ascorbate, and 3% (w/v) polyvinylpolypyrrolidone. After centrifuging to clear (18,000 x g, 20 min), 50% (w/v) polyethylene glycol 8000 was added to give a concentration of 30% (w/v), and the sample was held on ice for 30 min. After centrifuging (18,000 x g, 20 min), the pellet was discarded, and the supernatant was adjusted to 40% (w/v) polyethylene glycol, held on ice for 2 h, and recentrifuged (40,000 x g, 1 h). One-quarter of the resulting pellet (1.5 mg protein) was redissolved in 1.2 ml of 50 mM Tris-HCl, pH 8.0, 10 mM
-mercaptoethanol, and 20 mM NaCl and applied to a 1-ml Mono-Q column (Amersham Biosciences), which was developed with an NaCl gradient (20410 mM), collecting 0.3-ml fractions. pABA glucosyltransferase activity eluted in one major peak at
300 mM NaCl and in at least three minor peaks at lower NaCl concentration. The most active fraction (
100-fold enriched relative to the crude extract) was desalted on a 1-ml Sephadex G-25 column equilibrated with 25 mM Tris-HCl, pH 7.5, and 10 mM
-mercaptoethanol.
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RESULTS |
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The product migrating at Rf 0.4 was confirmed to be pABA-Glc, the
-D-glucopyranosyl ester of pABA (Fig. 2C), by UV spectroscopy (
max, = 299 nm), by release of pABA upon mild acid hydrolysis, by LC-MS ([M+NH4+] = 317), and by 1H NMR. The positions and coupling constants of characteristic 1H NMR aromatic and glucosyl resonances of the product (3.69 ppm, 1H, dd, J = 11 Hz, 5 Hz, H'-6a; 3.85 ppm, 1H, dd, J = 11 Hz,
1Hz, H'-6b; 5.65 ppm, 1H, d, J = 8 Hz; 6.63 ppm, 2H, d, J = 8 Hz, H-4 and H-6; 7.81 ppm, 2H, d,J = 8 Hz, H-3 and H-7) matched the previously reported NMR signals of pABA-Glc but not those of other plant metabolic derivatives of pABA (9, 11). The quality of the spectra was sufficient to rule out the presence of other pABA derivatives at levels exceeding 10% of the pABA-Glc.
In addition to the pABA-Glc band, TLC showed a diffuse labeled band at Rf 0.25, which is characteristic of the N-glucoside of pABA. Since this band was present in controls that received no [14C]pABA until extraction or that were heat-killed before incubation (Fig. 2B), and since the N-glucoside forms spontaneously and easily (13, 14), the Rf
0.25 band was taken to be N-glucoside of non-enzymatic and most likely postmortem origin and was not further analyzed.
Diverse Plants Accumulate pABA Glucose Ester but Bacteria and Yeast Do NotTo find whether pABA-Glc formation occurs in leaves as well as fruits and whether it is common among angiosperms, leaf samples of tomato and eight other species were given [14C]pABA for 6 h. In all cases, [14C]pABA uptake was nearly complete, the average being 93%, and label accumulated in pABA-Glc (Fig. 3, A and B). The proportion of absorbed pABA converted to pABA-Glc was in most species between 32 and 78%, which is similar to tomato fruit; only melon and cotton showed a lower conversion (1012%). As with tomato fruit, TLC analysis showed a labeled band in the pABA N-glucoside position in all samples. Two species (Arabidopsis and spinach) also had prominent low mobility labeled bands, presumably representing additional pABA metabolites (Fig. 3A). These bands were shown not to correspond to N-acetyl pABA (Rf, 0.62), p-hydroxybenzoate (Rf, 0.72), or folates (Rf 0) but were not further investigated in this study.
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To test whether pABA-Glc accumulates in other folate-synthesizing organisms, growing cultures of E. coli and yeast were given doses of [14C]pABA calculated to be well in excess of the pABA requirement for folate synthesis and incubated until >25% of the dose had been taken up. TLC analysis of methanol extracts of the harvested cells showed that ≤0.1% of the absorbed label was present as pABA-Glc. This negative result is consistent with the lack of reports of pABA-Glc in E. coli, whose pABA metabolism has been thoroughly studied (14, 28).
Much of the Endogenous pABA in Plants Is in Conjugated FormThat plant tissues rapidly convert exogenously supplied pABA to pABA-Glc implies that much of their endogenous pABA may exist in this form. To investigate this point, we analyzed free and conjugated forms of pABA by a protocol entailing methanol extraction, cation-exchange chromatography, ethyl acetate partitioning, and HPLC with fluorescence detection. A mild acid hydrolysis step was inserted after methanol extraction to release pABA from esters and other labile conjugates. The hydrolysis conditions (0.1 M HCl at 80 °C for 23 h) were selected to obtain essentially complete pABA release from pABA-Glc (and N-glucosyl pABA) without significant release of pABA from folates and without major losses of pABA via Maillard reactions with contaminating sugars. Since pABA-Glc undergoes some spontaneous hydrolysis during extraction and chromatography, representative samples were spiked with purified pABA-Glc to enable correction for such breakdown.
Fig. 4A is an illustrative HPLC fluorescence trace showing that the pABA peak is symmetrical, well resolved from other peaks, and substantially larger when the sample is hydrolyzed. Data of this type for tomato pericarp and for leaves of tomato, Arabidopsis, and two pea cultivars are summarized in Fig. 4B. For free pABA, both the corrected value and the correction, often large, applied for pABA-Glc breakdown are indicated (Fig. 4B). Folate contents are included for comparison (Fig. 4B, inset). Two sets of generalizations can be made from these data. First, the pABA contents after hydrolysis (hereafter termed total pABA) cover a wide span (from 0.2 to 11 nmol g1 of fresh weight) and vary from about 3600% of the folate content. Second, in all cases except tomato leaves (whose total pABA content is exceptionally low), the corrected free pABA values are ≤20% of total pABA, suggesting that ≥80% of pABA is in conjugated form in vivo.
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pABA Glucose Ester Is Formed by a Reversible UDP-glucosyltransferase ReactionUDP-glucose:pABA glucosyltransferase activity was readily detected in desalted extracts of mature green tomato pericarp and pea leaves, and the activities measured at near saturating substrate concentrations were sufficient to account for the observed in vivo rates of pABA-Glc formation (Fig. 5A). Almost no activity was detected when ADP-glucose replaced UDP-glucose (Fig. 5A). Using an enzyme preparation from pea leaves (100-fold enriched relative to the crude extract), the UDP-glucosyltransferase reaction was shown to be reversible (Fig. 5B), as has been reported for glucosyltransferases that produce other carboxyl esters (29, 30). While investigating the reverse reaction, we also detected modest pABA-Glc esterase activity in desalted crude extracts. The partially purified enzyme used to study the reverse reaction contained only a trace of this hydrolytic activity (Fig. 5B).
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pABA Glucosyltransferase and pABA-glucose Esterase Activities Are CytosolicThe subcellular locations of pABA glucosyltransferase and pABA-Glc esterase activities were investigated in pea leaves, which are well suited for recovery of intact organelles (25, 31). Chloroplasts and mitochondria were purified on Percoll gradients and showed little contamination from other fractions (Fig. 6). The preferred method for preparing minimally contaminated cytosol, via protoplast rupture, was unsuitable because of high pABA-Glc esterase activity in the commercial enzymes (cellulase, macerase, and pectinase) used in protoplast isolation. A cytosol-enriched fraction was therefore prepared by gentle mechanical grinding, centrifugation, and concentration. Such fractions are inevitably contaminated by chloroplastic enzymes, but this did not complicate data interpretation because glucosyltransferase and esterase activities were essentially absent from chloroplasts (Fig. 6). By far the highest specific activities of both these enzymes was in the cytosol fraction, and the activities in the mitochondrial fraction were low enough to be ascribed to cytosolic contamination (Fig. 6). We therefore conclude that the glucosyltransferase and esterase activities are both cytosolic, subject to the caveats that the cytosol fraction includes vacuolar enzymes and that a few percentages of both activities could be mitochondrial.
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DISCUSSION |
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Once inside plant cells, the anions of hydrophobic weak acids distribute themselves spontaneously among subcellular compartments according to pH, and therefore, flow in and out of chloroplasts, and consequently, other organelles, in response to the stromal pH changes that accompany light-dark transitions (36). Such light-driven redistribution within the cell may be undesirable for a crucial metabolic intermediate such as pABA. Another negative feature of hydrophobic weak acids is that they can act as uncouplers by shuttling across membranes and collapsing proton electrochemical gradients (37). A third drawback, specific to pABA, is that it is a substrate inhibitor of dihydropteroate synthase, the enzyme that couples it to a pterin moiety during folate synthesis. This inhibition occurs at µM levels of pABA for the bacterial enzyme (38) and apparently also for the plant enzyme (39). There are thus various a priori reasons for expecting plants to store pABA in a readily accessible form rather than as the free acid.
We propose that pABA-Glc formation serves this "accessible storage" function, based on four lines of evidence. First, pABA-Glc accumulation is widespread among plants and perhaps special to them as it was found in eight diverse angiosperm families but not in E. coli or yeast. Second, both radiotracer data and HPLC analyses indicate that the pABA-Glc pool is typically much larger than the pABA pool. Third, biochemical data show that pABA-Glc formation is reversible in two ways, via the bidirectionality of the synthetic reaction itself and via an independent hydrolytic activity. Last, subcellular distribution data show that pABA-Glc synthesis and reconversion to pABA take place in the cytosol, a plausible depot site for pABA en route between chloroplasts and mitochondria. This proposal for the role of pABA-Glc formation is summarized schematically in Fig. 7.
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The presence of pABA-Glc in plant cells invites the question of whether it is a substrate for membrane transporters. The tonoplast is an obvious potential site for a pABA-Glc transporter since plant vacuoles are known to import other aromatic glucose esters (40), and indirect evidence suggests that not all pABA-Glc is cytosolic. Thus, the highest pABA-Glc level reported for plant cells, 25 µmol g1 of fresh weight (10), would, if confined to the cytosol, produce the improbably high cytosolic concentration of
250 mM. The mitochondrial inner membrane is also a possible site for a pABA-Glc transporter (Fig. 7). If the cytosolic concentration of free pABA is extremely low, it might not drive enough pABA diffusion into mitochondria to sustain folate synthesis. Such a situation might be rectified by carrier-mediated pABA-Glc import into mitochondria, with concurrent or subsequent hydrolysis. This scenario might seem far-fetched in the light of HPLC data indicating significant levels of free pABA. However, it should be noted that the free pABA data are in most cases imprecise because they involve such large corrections for pABA-Glc breakdown. Moreover, the estimates of free pABA entail the assumption that all conjugated pABA is in the form of pABA-Glc. If some pABA exists in vivo as the N-glucoside (which our data cannot exclude), then because the N-glucoside breaks down more extensively during sample processing than pABA-Glc, free pABA would be overestimated. In short, although free pABA values are plainly in most cases low relative to total pABA, exactly how low is uncertain. In one case at least (Arabidopsis leaves), they are so low as to be undetectable against the background of spontaneous pABA-Glc breakdown.
Lastly, the data on pABA content of tomato fruit relate to the engineering of enhanced folate content. The total pABA content of mature green fruit is 1.8 nmol g1 of fresh weight (Fig. 4B) as compared with a folate content of 1 nmol g1 of fresh weight (17). The standing pool of pABA moieties is therefore not enough to support more than a 180% increase in folate content, which is less than the increase desired. Thus, if pABA synthesis in plants, as in bacteria (41), is not feedback regulated by pABA, it may need to be boosted as part of the engineering process.
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FOOTNOTES |
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|| To whom correspondence should be addressed: Horticultural Sciences Dept., University of Florida, P. O. Box 110690, Gainesville, FL 32611. Tel.: 352-392-1928 (ext. 334); Fax: 352-392-5653; E-mail: adha{at}mail.ifas.ufl.edu.
1 The abbreviations used are: pABA, p-aminobenzoic acid; pABA-Glc, p-aminobenzoyl -D-glucopyranoside; HPLC, high performance liquid chromatography; LC-MS, liqiud chromatography-mass spectrometry.
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REFERENCES |
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