From the
Department of Medicine, and Center for Molecular and Cellular Neuroscience, Vanderbilt University Medical Center, Nashville, Tennessee 37232,
Department of Pharmacology, and Center for Molecular and Cellular Neuroscience, Vanderbilt University Medical Center, Nashville, Tennessee 37232
Received for publication, October 28, 2002
, and in revised form, April 2, 2003.
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ABSTRACT |
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INTRODUCTION |
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MUPP1, a protein containing 13 putative PDZ domains, was isolated in a yeast two-hybrid screening for proteins that bound to the carboxyl-terminal tail of the 5-HT2C R (1). MUPP1 is expressed in many tissues, whereas the 5-HT2C R is a brain-specific protein (1, 11). The 5-HT2C R has classically been thought to couple to Gq activation; however, additional G protein families have been implicated, leading to the activation of different downstream signaling pathways including phospholipase A2, C, or D, and various cation channels (1217). Since PDZ-containing proteins can scaffold many signaling molecules together into a signal transduction complex, the interaction between MUPP1 and the 5-HT2C R was further investigated. The 5-HT2C R contains a PDZ binding motif, Ser458-Ser-Val, at its extreme carboxyl terminus, which is critical for interaction with PDZ 10 of MUPP1 (18). In an alternate approach to the yeast two-hybrid system, we independently show that PDZ 10 of MUPP1 is the primary site of interaction for the 5-HT2C R.
Serotonin stimulation has previously been shown to promote phosphorylation of the two serine residues of the 5-HT2C R PDZ binding motif, Ser458 and Ser459 (2). We therefore hypothesize that phosphorylation of the carboxyl-terminal serines of the 5-HT2C R regulates receptor interaction with MUPP1. To test this hypothesis, we investigated whether a modification of Ser458 and/or Ser459 of the 5-HT2C R carboxyl-terminal tail would alter PDZ 10 interaction. Ser458 and/or Ser459 of the receptor tail were mutated to aspartate to mimic phosphorylation (i.e. introduction of a negative charge). Next, cells expressing 5-HT2C Rs were treated with agonist or antagonist to assess the interaction of the 5-HT2C R with MUPP1. The results of these experiments support our hypothesis that phosphorylation is a key regulator of 5-HT2C R interaction with MUPP1. Furthermore, the results indicated that a significant amount of basal phosphorylation of the receptor may also play a yet undetermined role in regulating PDZ-protein interactions.
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MATERIALS AND METHODS |
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DNA Constructs
Overlapping regions of MUPP1 containing two or three PDZ domains (Fig. 1), or one PDZ domain (PDZ 9, 10, or 11) were generated by reverse transcription-PCR, sequenced, and subcloned into pGEX-4T1 (Amersham Biosciences) for expression of GST fusion proteins. MUPP1 PDZ 9, 10, or 11 were also subcloned into pGEMEX-1 (Promega), a T7 gene 10 fusion protein vector. MUPP1 PDZ 911 was also subcloned into pcDNA3 (Invitrogen). The 5-HT2C R carboxyl-terminal tail (last 60 amino acids) with or without the PDZ binding motif (Ser458-Ser-Val) and a truncation mutant at residue 445 were subcloned into pGEMEX-1. The 5-HT2C R carboxyl-terminal tail with the PDZ binding motif was also subcloned into pGEX-4T1. The 5-HT2C R carboxyl tail Ser458-Ser-Val (WT) was modified to S458A, S458D, S459D, or S458D/S459D by PCR site-directed mutagenesis and subcloned into pGEMEX-1.
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GST Fusion Protein Overexpression
Escherichia coli was transformed with pGEX-4T1 constructs, induced to overexpress fusion proteins with isopropyl -D-thiogalactoside, and analyzed. Bacterial lysates were obtained by first adding cold lysis buffer (50 mM Tris pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM dithiothreitol, 1 µg/ml leupeptin, 1 µg/ml pepstatin A, 1 µg/ml aprotinin, 1 mM benzamide, 1 mM benzamidine, 1 mM phenylmethylsulfonyl fluoride) to resuspend the pellets. Resuspended pellets were sonicated for 20 s on ice and centrifuged at 15,000 rpm for 30 min at 4 °C. Proteins were resolved on SDS-PAGE to confirm overexpressed GST fusion protein by Coomassie Blue staining and Western blotting using GST antibodies.
[35S] in Vitro Translation
pGEMEX-1 constructs were used for coupled transcription and translation using the TNT® in vitro translation system (Promega) in the presence of [35S]methionine (PerkinElmer Life Sciences) according to the supplier's protocol to generate 35S-labeled proteins.
Protein Overlay Assays
Ten micrograms of GST fusion proteins or GST were size-fractionated on SDS-PAGE and transferred onto nitrocellulose membrane. Nitrocellulose membranes were blocked with freshly prepared 1% BSA/phosphate-buffered saline for 1 h at room temperature. Solution was then replaced with 35S-labeled fusion proteins in 1% BSA/phosphate-buffered saline buffer and incubated with nitrocellulose membranes for 16 h at 4 °C. Nitrocellulose membranes were rinsed three times for 20 min at room temperature in 1% BSA/phosphate-buffered saline containing 0.2% Triton X-100. Nitrocellulose membranes were air-dried and exposed to x-ray film or a PhosphorImager screen (Amersham Biosciences) to visualize radiolabeled proteins. Western blot analysis using GST antibodies was used to document similar GST protein levels.
Cell Culture
NIH-3T3 cells stably transfected with the 5-HT2C R (5-HT2C R/3T3) were cultured in Dulbecco's modified Eagle's medium (Invitrogen) until confluent (20). Cells were washed four times with Hanks' buffered saline solution (with Ca2+/Mg2+) and then serum starved in serum-free Dulbecco's modified Eagle's medium for 16 h. Cells were treated without or with antagonist (1 µM 2-bromolysergic acid diethylamide (BOL)) for 15 min at 37 °C prior to serotonin addition for 30 min at 37 °C. Medium was then removed, 1 ml of Tris buffer (50 mM Tris, pH 7.6, 0.5 mM EDTA, pH 8.0, 5 µM leupeptin, 1 mM phenylmethylsulfonyl fluoride) was added, and cells were scraped from plates and placed in an Eppendorf tube on ice. Membrane extracts were obtained using 300 µl of Tris buffer containing 1% Triton X-100. Membrane protein concentrations were determined by BCA protein assay (Pierce). Equal amounts of protein were added to the affinity columns. Western blot analysis using GST antibodies was used to determine that similar levels of fusion protein were pulled down.
Pull-down Assays
Twenty microliters of glutathione-Sepharose beads (Amersham Biosciences) were washed three times with PD buffer (20 mM HEPES, pH 7.6, 100 mM KCl, 10% glycerol, 0.5 mM EDTA, pH 8.0, 1 mM phenylmethylsulfonyl fluoride, 1 mM dithiothreitol, 1% Nonidet P-40). Ten micrograms of GST fusion proteins or GST were incubated with the washed glutathione beads for 1 h at 4 °C. Five microliters of 35S-labeled fusion proteins or 50 µg of membrane extracts were added to the GST-glutathione beads and incubated for 23 h or overnight, respectively, at 4 °C. After incubation, GST-glutathione beads were washed six times (for assays with 35S-labeled fusion proteins) or three times (for assays with membrane extracts) with PD buffer. For pull-downs from 5-HT2C R/3T3 cell lysates, precipitated protein, containing the 5-HT2C R, was treated with peptide:N-glycanase F before SDS-PAGE (see below). Loading dye (6% SDS, 1% -mercaptoethanol, 20 mM Tris, pH 6.8, 10% glycerol plus a little bromphenol blue) was added to elute proteins. Eluates were separated on SDS-PAGE and transferred onto nitrocellulose membranes. Autoradiography or PhosphorImager screen was used to visualize radiolabeled proteins. Western blot analysis using GST and 5-HT2C R antibodies were used to document GST fusion proteins and 5-HT2C R, respectively.
Agonist Washout
After serum starvation, cells were treated with 100 nM serotonin for 30 min at 37 °C. After treatment, medium was aspirated to remove the serotonin, and cells were washed four times with Hanks' buffered saline solution. Serum-free Dulbecco's modified Eagle's medium was then added, and cells were incubated for 10 or 30 min. Cells were then lysed, and a pull-down assay was performed as mentioned above.
Band Shift Phosphorylation Assay
This assay was performed as previously described by Backstrom et al. (2). Briefly, cells grown to confluence were serum-starved and treated with increasing amounts of serotonin for 15 min at 37 °C. This incubation time was previously shown to be the minimal amount of time that would result in maximal receptor phosphorylation. Cells were then lysed, and membrane extracts containing receptors were prepared. Western blot analysis using 5-HT2C R antibodies were used to document changes in 40- and 41-kDa bands, representative of unphosphorylated and phosphorylated 5-HT2C R, respectively.
Deglycosylation
Following pull-downs from membrane extracts, beads were pelleted and washed once with PD buffer containing 0.1% SDS. Fifteen microliters of PD buffer containing 1% SDS was added to the beads, and the mixture was incubated for 15 min at 37 °C. Then 58 µl of PD buffer was added, and after mixing, 15 µl of PD buffer containing 10% Triton X-100 was added. Finally, 2 µl of peptide:N-glycanase F (Glyko or New England Biolabs) was added, and samples were incubated for 2 h at 37 °C. After deglycosylation, 15 µl of 4x loading dye were added, and samples were incubated at room temperature for 20 min prior to SDS-PAGE.
Dephosphorylation
Membrane extracts (50 µg) of untreated or serotonin-treated cells were incubated in PD buffer plus 50 units of calf intestinal (alkaline) phosphatase (New England Biolabs) for 2 h at room temperature. After incubation, pull-down assays were carried out as described above.
Western Blot Analysis
Alkaline Phosphatase DetectionNitrocellulose membranes were blocked in 1% BSA/Tris blot buffer (25 mM Tris, pH 7.5, 150 mM NaCl, 0.05% Tween 20, 0.05% NaN3)for1hat room temperature. Membranes were then incubated with GST (1:1000 dilution) or 5-HT2C R (35 µg/ml) antibodies in 1% BSA/Tris blot buffer for 2 h to overnight at 4 °C. Membranes were washed three times with Tris blot buffer alone for 10 min. Alkaline phosphatase-conjugated goat anti-rabbit secondary antibodies (1:1000 dilution; Jackson Immunolaboratories) were incubated with membranes for2hat room temperature. Membranes were washed three times with Tris blot buffer and once with Tris (150 mM pH 9.4) and then developed with 5-bromo-4-chloro-3-indolyl-phosphate and nitro blue tetrazolium.
Chemilluminescence DetectionNitrocellulose membranes were blocked in 8% milk/Tween 20 Tris buffer solution (25 mM Tris, pH 7.4, 137 mM NaCl, 0.27 mM KCl, 0.05% Tween 20) overnight at 4 °C. Membranes were then incubated with GST (1:4000 dilution) or 5-HT2C R (0.5 µg/ml) antibodies in 2% milk/Tween 20 Tris buffer solution overnight at 4 °C. Membranes were washed four times for 5 min with Tween 20 Tris buffer solution. Horseradish peroxidase-conjugated goat anti-rabbit secondary antibodies (1:20,000 dilution; Jackson Immunolaboratories) were incubated with membranes for 45 min at room temperature. Membranes were washed four times for 15 min with Tween 20 Tris buffer solution and developed with the Pierce Supersignal West Dura® kit according to the supplier's protocol. Horseradish peroxidase signal was analyzed by Bio-Rad Flouro-S, and densitometric analysis was performed by QuantityOne (Bio-Rad) software.
Statistical Analysis
All bar graph data was analyzed with Graphpad Prism one-way analysis of variance with Tukey's post-test; p < 0.05 is significant, unless otherwise noted in a figure legend. GST alone was the background control for all GST fusion protein experiments, and graph data presented are background-subtracted. Data represent the means ± S.D. from several independent experiments.
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RESULTS |
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To further determine the specific site of interaction, GST fusion proteins of the individual PDZ domains, 9, 10, and 11 were made (Fig. 1). Unfortunately, GST-PDZ 10 was unstable when overexpressed in bacteria. Therefore, the ability to pull down the 35S-labeled 5-HT2C R tail by GST-PDZ 9 or 11 was compared with GST-PDZ 911. GST fusion proteins of PDZ 9 or 11 alone were not able to bind to the receptor tail as compared with GST-PDZ 911, which contains PDZ 10 (data not shown). In a complementary pull-down experiment, the individual MUPP1 PDZ domains 9, 10, and 11 were in vitro translated with [35S]methionine and pulled down by the 5-HT2C R carboxyl-terminal tail expressed as a GST fusion protein (Fig. 2B). The carboxyl tail of the receptor specifically interacted with PDZ 10 and not PDZ domain 9 or 11, further supporting PDZ 10 as the interacting region for the receptor tail.
Next, we questioned whether regions upstream of the extreme carboxyl terminus of the 5-HT2C R are able to confer binding to PDZ 10. To address this question, we generated GST fusion proteins of the 5-HT2C R carboxyl-terminal tail missing only the last three residues ( PDZ) and the 5-HT2C R carboxyl-terminal tail ending at residue 445 (i.e. missing the last 15 amino acids). In an overlay assay, [35S]PDZ 911 was incubated with the different GST-5-HT2C R carboxyl-terminal tail fusion proteins. As illustrated in Fig. 3, PDZ 911 binds to WT but not the 5-HT2C R carboxyl-terminal truncation mutants.
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Mutation of Ser458 in the 5-HT2C Receptor Reveals Altered PDZ 10 InteractionStudies previously demonstrated that Ser458 and Ser459 at the extreme carboxyl tail of the 5-HT2C R, the same region of the receptor necessary for PDZ 10 binding, are phosphorylated upon ligand activation (2). A function for Ser459 phosphorylation in receptor resensitization was proposed; however, the role for Ser458 phosphorylation is unknown. Based upon crystal structures of PDZ domains (2125) and data compiled on PDZ binding motifs (26), Ser458 of the 5-HT2C R is predicted to be a critical residue for interacting with PDZ 10. We therefore hypothesized that agonist-mediated phosphorylation of Ser458 disrupts the interaction of MUPP1 PDZ domain 10 and its target, the 5-HT2C R. To test this hypothesis, the serine residues in the receptor tail were replaced with aspartic acid to mimic phosphorylation. The last two serine residues of the 5-HT2C R carboxyl tail (Ser458-Ser459) were modified by PCR site-directed mutagenesis to contain S458A, S458D, S459D, or S458D/S459D substitutions. Wild-type and mutated 5-HT2C R tails were labeled with [35S]methionine and incubated with GST-PDZ 911. 5-HT2C R tail mutants containing S458A, S458D, and S458D/S459D substitutions displayed a marked loss of interaction to PDZ 911 (Fig. 4A). The S459D mutation, however, retained an ability to interact similar to wild-type interaction (Fig. 4B). These results indicate that Ser458 is an important residue in determining the interaction with PDZ 10.
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Serotonin Treatment Decreases the Ability of the 5-HT2C Receptor to Interact with PDZ 10 Results from the 5-HT2C R tail mutants raise the possibility of a dynamic regulation of the interaction between the 5-HT2C R and MUPP1. Thus, we investigated whether agonist stimulation of the 5-HT2C R stably expressed in NIH-3T3 cells would also result in a loss of MUPP1 interaction. To determine whether serotonin stimulation had any effect on MUPP1-receptor interaction, cells were incubated with increasing amounts of serotonin, which have been shown to promote receptor phosphorylation (2). The ability of the 5-HT2C R to bind to PDZ 10 was assessed by pull-down assays. Fig. 5A shows that cells treated with serotonin led to a dose-dependent decrease in receptor interaction with MUPP1. A 50% reduction in receptor binding to PDZ 10 was observed with a concentration 100 nM serotonin. Moreover, increasing serotonin concentrations caused a dose-dependent increase in phosphorylated receptor with a concurrent decrease in the amount of unphosphorylated receptor as determined by band shift phosphorylation assays (Fig. 5B).
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To determine whether the loss of PDZ 10 interaction with the receptor was a consequence of agonist binding with 5-HT2C Rs, cells were preincubated in the absence or presence 1 µM of BOL, a 5-HT2C R antagonist, for 15 min prior to the addition of serotonin. BOL antagonized a subsequent serotonin-mediated decrease in receptor pull-down (Fig. 6), thereby demonstrating that the loss of PDZ 10 interaction is a direct consequence of receptor activation. BOL alone had no effect.
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Alkaline Phosphatase Treatment of the 5-HT2C Receptor Increases PDZ 10 Interaction and Reveals 5-HT2C Receptor Basal PhosphorylationThe reduction of 5-HT2C R binding to MUPP1 may be the direct result of receptor phosphorylation. We therefore investigated whether treatment of lysate containing receptor with alkaline phosphatase would restore MUPP1 interaction. Cells were treated with agonist, and cell lysates were incubated with alkaline phosphatase prior to pull-down assays. As shown in Fig. 7A, alkaline phosphatase treatment resulted in more receptor pull-down in serotonin-stimulated cells. In the absence of serotonin, alkaline phosphatase treatment doubled the amount of receptor binding to PDZ 10 compared with untreated cells. These findings directly support a role for agonist-induced phosphorylation in disrupting 5-HT2C R binding to MUPP1 as well as uncover a potential function for previously reported basal phosphorylation of the receptor.
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Phosphorylation of the receptor is reversible; therefore, we investigated the activity of endogenous phosphatases against the receptor by washout experiments. Cells were treated with agonist and then washed thoroughly and incubated in serum-free medium for 10 or 30 min before lysis and pull-down assay. Fig. 7B demonstrates a time-dependent increase in 5-HT2C R binding to MUPP1 (Fig. 7B). These results are consistent with previously published data indicating a time-dependent dephosphorylation of the receptor (2).
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DISCUSSION |
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In the current paper, 5-HT2C R interaction with PDZ 10 of MUPP1 was independently confirmed by using pull-down and protein overlay assays. These results are consistent with the report by Becamel et al. (18) that demonstrated PDZ 10 interaction with the 5-HT2C R in yeast two-hybrid assays. Becamel et al. (18) also demonstrated that mutation of the critical residues of the PDZ motif on the receptor, Ser458 or Val460 to alanine, abolished interaction with PDZ 10. Similarly, our results also document that the carboxyl-terminal residues of the 5-HT2C R are necessary for MUPP1 interaction; truncated 5-HT2C R tail proteins missing the terminal PDZ binding motif failed to show detectable interaction with MUPP1. There are four recognized classes of carboxyl-terminal PDZ binding motifs to date (48). The carboxyl terminus of the 5-HT2C R belongs to a type 1 PDZ binding motif (X-Ser/Thr-X-Val/Ile/Leu-COOH). Ser458 of the 5-HT2C R is predicted to be a critical residue for interacting with the PDZ 10 domain backbone. Since both Ser458 and Ser459 of the 5-HT2C R are phosphorylated upon ligand activation (2), we examined the role of receptor phosphorylation on the 5-HT2C R-MUPP1 interaction.
In the next series of experiments, we investigated whether mutations of the terminal serines to aspartates that mimic phosphorylation of serines would affect binding of the 5-HT2C R to PDZ 10 of MUPP1. S458D and S458D/S459D mutations led to a significant loss of receptor tail interaction to PDZ 10. These data are in agreement with crystal structure data indicating that the hydroxyl group of the Ser at the 2 position of a type I PDZ binding target is important for hydrogen bonding to His at the first position of the B helix (
B1) of a type I PDZ domain such as PDZ 10 (21). Our data suggest that the introduction of a negatively charged group at the 2 position of the receptor tail disrupts the hydrogen bonding to the PDZ 10 domain resulting in a loss of interaction. The S459D mutation, on the other hand, retains wild-type interaction. This result is also consistent with structural studies showing that the side chain of the 1 position residue of a PDZ binding target does not hydrogen-bond to residues in the PDZ domain backbone but rather points away from the pocket (21). Interestingly, c-Kit, a receptor tyrosine kinase whose carboxyl-terminal sequence ends in Asp-Asp-Val, and claudin-1, a tight junction protein whose carboxyl-terminal sequence ends in Asp-Tyr-Val, have been identified to interact with PDZ 10 of MUPP1 (49, 50). These observations are unexpected in light of structural information on PDZ-ligand complexes, including the current data of carboxyl-terminal mutants in the 5-HT2C receptor. One possible explanation is that c-Kit and claudin-1 interaction with PDZ 10 may involve additional residues upstream from the carboxyl termini.
To determine whether serotonin stimulation within a cellular context would alter 5-HT2C R binding to PDZ 10, we examined the receptor stably expressed in NIH-3T3 cells in the absence or presence of serotonin. A dose-dependent loss of interaction of the receptor with MUPP1 occurred when receptor was incubated with increasing concentrations of serotonin. Furthermore, this concentration curve parallels serotonin-induced receptor phosphorylation, suggesting that a change in receptor phosphorylation state regulates binding to MUPP1 PDZ 10. The effect of serotonin was blocked by the 5-HT2C R antagonist, BOL. Moreover, alkaline phosphatase treatment reversed the effect of serotonin. Interestingly, in the absence of serotonin, phosphatase treatment also resulted in increased receptor binding to PDZ 10. We speculate that this may be indicative of constitutive phosphorylation; basal phosphorylation for this receptor was previously observed by Westphal et al. (51) when cells were metabolically labeled with [32P]orthophosphoric acid. Alternatively, an unidentified protein that attenuates MUPP1 binding to the receptor may be reduced by phosphatase treatment and account for this observation. When receptor was stimulated with a maximal serotonin-mediated receptor phosphorylation dose, agonist washout experiments demonstrated that receptor interaction with MUPP1 was restored in a time-dependent manner. We speculate that this restored binding between the receptor and MUPP1 is the result of endogenous phosphatases. Results from antagonist, phosphatase treatments, and agonist washout experiments demonstrated the specificity of our analyses and support the hypothesis that phosphorylation regulates the interaction of 5-HT2C R with MUPP1. A thorough investigation of the kinase responsible for phosphorylation of the 5-HT2C R has been initiated. Initial studies suggest that the usual second messenger-activated kinases are not involved (data not shown). This finding is consistent with investigations of another G-protein-coupled receptors, the -adrenergic receptors, which have identified GRK2 and GRK5 as the major kinases responsible for phosphorylating receptor carboxyl threonine and serine residues (40, 52, 53).
Regulation of PDZ-protein interactions by phosphorylation is emerging as an important modulator of cellular signaling. Phosphorylation regulation of a PDZ domain interaction was first observed in the potassium channel Kir 2.3 (37). Protein kinase A phosphorylation of Kir 2.3 causes a disruption of its interaction with PSD-95. Protein kinase A also regulates the interaction between Kir 2.2, another potassium channel, and synapse-associated protein 97, SAP97, resulting in a decreased interaction (38). G protein-coupled receptor kinases have been shown to phosphorylate the 1- and
2-adrenergic receptors at carboxyl-terminal serines of a PDZ binding motif, which reduces their interaction with PDZ domain proteins (39, 40, 54). The
-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate (AMPA)glutamate receptor exhibits dual specificity for its interacting partners PICK1 (protein interacting with C kinase) and GRIP/ABP (glutamate receptor-interacting protein)/(AMPA receptor-binding protein), depending upon whether the glutamate receptor 2/3 subunit is phosphorylated by protein kinase C (4245, 55, 56). Finally, the binding of stargazin, a transmembrane protein that associates with AMPA receptors, to PSD-95 is regulated by protein kinase A phosphorylation (46, 47, 57, 58).
In this paper, we established that phosphorylation of the 5-HT2C R at Ser458, the serine residue critical for binding to PDZ 10, resulted in a decrease in MUPP1 interaction. We also provide evidence that receptor interaction with MUPP1 is sensitive to basal phosphorylation of the receptor. Due to the assembling nature of PDZ proteins, we propose that MUPP1 regulates the coupling of the 5-HT2C R to various effectors to activate downstream signaling processes. MUPP1 interaction with the 5-HT2C R when the receptor is in an unphosphorylated form may keep the receptor in a conformation state (and vice versa) that is masked from some of its downstream signaling partners. Then in an agonist-dependent or -independent manner (an event that is not fully understood), the receptor becomes phosphorylated at the PDZ binding motif, and MUPP1 is released from the receptor, resulting in a change in conformation that reveals downstream signaling molecules scaffolded to MUPP1. Changes in MUPP1 folding have been postulated as a result of observations by Becamel et al. (18), showing that when MUPP1 is expressed alone in COS cells, a carboxyl-terminal vesicular stomatitis virus epitope tag is not accessible; however, when MUPP1 is co-expressed with the 5-HT2C R, the tag is accessible. The possibility also exists that the sole function of MUPP1 may be to simply traffic or anchor the 5-HT2C R to specific membrane domains without a direct effect upon receptor signaling. Based upon the data presented here and previous studies on PDZ proteins and receptors, phosphorylation appears to be a critical regulator of PDZ protein-protein interactions, including regulation of the interaction between MUPP1 and the 5-HT2C R. Our results suggest that cells may have an underlying mechanism to dynamically regulate overall cellular activity in the absence and subsequent exposure to agonist by modulating basal receptor phosphorylation and thereby balancing the extent of maximal receptor activation.
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FOOTNOTES |
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¶ To whom correspondence should be addressed: 402 Robinson Research Bldg., Vanderbilt University Medical Center, 23rd Ave. at Pierce, Nashville, TN 37232. Tel.: 615-343-0441; Fax: 615-343-6532; E-mail: Bih-Hwa.Shieh{at}vanderbilt.edu.
1 The abbreviations used are: PDZ, postsynaptic density-95, Discs large, zonula occludens-1; MUPP1, multiple PDZ domain protein 1; 5-HT2C R, serotonin2C receptor; MAGUK, membrane-associated guanylate kinase; GST, glutathione S-transferase; BOL, 2-bromo-lysergic acid diethylamide; AMPA, -amino-3-hydroxyl-5-methyl-4-isoxazolepropionate; WT, wild type.
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ACKNOWLEDGMENTS |
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REFERENCES |
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