RNA Interference Reveals That Endogenous Xenopus MinK-related Peptides Govern Mammalian K+ Channel Function in Oocyte Expression Studies*

Arun AnantharamDagger§, Anthony LewisDagger§, Gianina Panaghie§||, Earl Gordon§||, Zoe A. McCrossan§, Daniel J. Lerner§, and Geoffrey W. Abbott§**

From the § Division of Cardiology, Departments of Medicine and Pharmacology,  Graduate Program of Neuroscience, and || Graduate Program of Pharmacology, Weill Medical College of Cornell University, New York, New York 10021

Received for publication, December 16, 2002, and in revised form, January 14, 2003

    ABSTRACT
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The physiological properties of most ion channels are defined experimentally by functional expression of their pore-forming alpha  subunits in Xenopus laevis oocytes. Here, we cloned a family of Xenopus KCNE genes that encode MinK-related peptide K+ channel beta  subunits (xMiRPs) and demonstrated their constitutive expression in oocytes. Electrophysiological analysis of xMiRP2 revealed that when overexpressed this gene modulates human cardiac K+ channel alpha  subunits HERG (human ether-a-go-go-related gene) and KCNQ1 by suppressing HERG currents and removing the voltage dependence of KCNQ1 activation. The ability of endogenous levels of xMiRP2 to contribute to the biophysical attributes of overexpressed mammalian K+ channels in oocyte studies was assessed next. Injection of an xMiRP2 sequence-specific short interfering RNA (siRNA) oligo reduced endogenous xMiRP2 expression 5-fold, whereas a control siRNA oligo had no effect, indicating the effectiveness of the RNA interference technique in Xenopus oocytes. The functional effects of endogenous xMiRP2 silencing were tested using electrophysiological analysis of heterologously expressed HERG channels. The RNA interference-mediated reduction of endogenous xMiRP2 expression increased macroscopic HERG current as much as 10-fold depending on HERG cRNA concentration. The functional effects of human MiRP1 (hMiRP1)/HERG interaction were also affected by endogenous xMiRP2. At high HERG channel density, at which the effects of endogenous xMiRP2 are minimal, hMiRP1 reduced HERG current. At low HERG current density, hMiRP1 paradoxically up-regulated HERG current, a result consistent with hMiRP1 rescuing HERG from suppression by endogenous xMiRP2. Thus, endogenous Xenopus MiRP subunits contribute to the base-line properties of K+ channels like HERG in oocyte expression studies, which could explain expression level- and expression system-dependent variation in K+ channel function.

    INTRODUCTION
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Voltage-gated potassium (Kv)1 channels form an aqueous pore through the plasma membrane by the assembly of four alpha  subunits, each with six transmembrane helices and a pore loop (1). The favored method for evaluating potassium channel function is by microinjection of channel cRNA into Xenopus laevis oocytes to facilitate two-electrode voltage clamp (TEVC) or patch clamp studies. However, observed differences in the behavior of potassium channel genes when expressed in oocytes compared with mammalian cells suggest that endogenous factors in either or both systems dictate channel properties to some extent (2-5). Studies of cardiac Kv channels HERG and KCNQ1 involve a further level of complexity because they co-assemble with transmembrane beta  subunits, designated KCNE subunits, or MinK-related peptides (MiRPs), to generate some native currents (6-10). The mammalian KCNE superfamily was cloned by a BLAST search of EST data bases using the sequence for MinK encoded by KCNE1 (6, 11, 12). Co-assembly of HERG with MiRP1 encoded by KCNE2 forms a channel with the properties of the IKr cardiac repolarization current (6, 11, 13-18), and KCNQ1 co-assembles with MinK to form cardiac IKs (8, 10). The necessity of alpha  and beta  subunits alike in human physiology is highlighted by the linkage of mutations in both to cardiac arrhythmia (6, 15, 17, 19-24). MiRP1, for example, is thought to play a central and diverse role in human cardiac physiology on the basis of cardiac expression (6, 25, 26), association with cardiac arrhythmia (6, 15, 17), and ability to modulate not only HERG but also KCNQ1 (26, 27), Kv4.2 (cardiac Ito) (16), and even the distantly related HCN pacemaker channels (28). Further, because beta  subunits alter the sensitivity and functional effects of both therapeutic and unwanted drug interactions and are linked to drug-induced long QT syndrome, the assignment of both alpha  and beta  subunits to native currents is essential for drug screening and rational drug design (6, 15).

Previous studies using either Xenopus oocytes or mammalian cells as expression systems have produced conflicting reports and often no consensus as to the functional consequences of alpha -beta subunit interactions, particularly in the case of HERG, which interacts with human MinK, MiRP1, and MiRP2 (6, 9, 29). In addition, many investigators report that within a single study the properties of heterologously expressed MiRP/alpha subunit currents vary with the expression system or with the concentration of MiRP cRNA, even at levels where alpha -subunit saturation would be expected (6, 16, 17). This finding suggests that these interactions depend on factors intrinsic to the expression system used. Clearly, resolving the true heteromultimeric identity of native ion channels and currents recorded in heterologous expression studies is important for our understanding of channel structure-function and physiology. Here we cloned a family of xMiRPs endogenously expressed in Xenopus oocytes and demonstrated that xMiRP2 interacts with overexpressed HERG to shape the functional attributes of HERG during oocyte expression studies.

    EXPERIMENTAL PROCEDURES
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
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Molecular Biology-- xMinK, xMiRP2, xMIRP4.1, and xMiRP4.2 were cloned from cDNA isolated by RT-PCR from stages V and VI X. laevis oocyte mRNA using primer sequences as follows: xMinK, 5'-ATGCCAGGGTTAAACACCACTGCC-3' (forward) and 5'-CTAGTTGCTGGG AGAAGAGGGGATATA-3'(reverse); XMIRP2, 5'-CAGTTTGATTGGAGAGTGGGATTC-3' (forward) and 5'-TAGACCCCTGGGGCCTCGTC-3' (reverse); xMIRP4.1, 5'-ATGAATTGTAGTAATACTTCTC GT-3' (forward) and 5'-CTAAAGTAAGTTTTCACTTTCAATACT-3' (reverse); xMIRP4.2, 5'-CGGGAATCAGACTTCTGTCC-3' (forward) and 5'-GCTTTACAGGATACACAGTAGC-3' (reverse). Genes were identified by a BLAST search of EST data bases with mammalian KCNE gene sequences using the tblastn algorithm. All genes showed a canonical initiation ATG site with G at -3, A at +3, and no intervening methionines 3' of the nearest 5' stop codon. For functional expression, xMiRP2 was ligated into pGA1 and linearized with SacI for transcription of cRNA for injection into oocytes. For studies in oocytes, cRNA transcripts for xMiRP2, human MiRP1 (hMiRP1) (in pGA1), hKCNQ1 (in a pBluescript-based vector), and HERG (in pSP64, Promega) were produced as described previously (6). To avoid dilution errors, all oocytes were injected first with the appropriate alpha  subunit cRNA and then immediately afterward with modifying beta  subunits or short interfering RNA (siRNA). For studies in CHO cells, cDNAs in pCINeo (Promega) were co-transfected with green fluorescent protein (in pBOB) using Superfect transfection reagent (Qiagen), and currents were recorded 24-36 h later.

For RNAi, 500 pg of double-stranded siRNA 21-mer oligos (Dharmacon) corresponding to bases 104-124 of xMiRP2 (top strand 5'-AAGGGAACCACACGGACGCCA-3') was injected into oocytes immediately after the injection of alpha  subunit cRNA. siRNA oligo for xMiRP2 was co-transfected into CHO cells with the appropriate channel cDNA. For the assessment of RNAi gene silencing at the mRNA level, an equal amount of oocytes was injected either with xMiRP2 cRNA or scrambled control siRNA, and RNA was extracted using Trizol (Invitrogen) on the same day that functional experiments were carried out and on the same batch of oocytes. RNA concentration was measured by spectrophotometry (SmartSpec3000; Bio-Rad), and RT-PCR was performed with Xenopus beta -actin primers (forward, 5'-AAGGAGACAGTCTGTGTGCGTCCA-3'; reverse, 5'-CAACATGATTTCTGCAAGAGCTCC-3') to normalize mRNA concentration. The optical density of samples run on a 1% agarose gel and stained with ethidium bromide was measured using a Fluor-S MultiImager (Bio-Rad). Samples normalized to beta -actin cDNA concentration were subsequently amplified using xMiRP2-specific primers, and optical density was quantified.

Electrophysiology-- Whole-cell currents in oocytes were recorded at room temperature by TEVC using an OC-725C Amplifier (Warner Instruments, Hamden, CT), an IBM computer, and pCLAMP8 software (Axon Instruments, Foster City, CA). Data analysis was performed using CLAMPFIT (Axon Instruments). Bath solution was (in mM) 4 KCl, 96 NaCl, 0.7 MgCl2, 1 CaCl2, and 10 HEPES (pH 7.4). Whole-cell currents were recorded at room temperature in CHO cells using a Multiclamp 700A Amplifier (Axon Instruments), an IBM computer, and pCLAMP8 software. Cells were studied on an inverted microscope equipped with epifluorescence optics for green fluorescent protein detection. The bath solution was composed of (in mM) 135 NaCl, 5 KCl, 1.2 MgCl2, 5 HEPES, 2.5 CaCl2, and 10 D-glucose (pH 7.4). Pipettes were of 3-5 Megaohms resistance when filled with intracellular solution containing (in mM) 10 NaCl, 117 KCl, 2 MgCl2, 11 HEPES, 11 EGTA, and CaCl2 (pH 7.2).

Biochemistry-- Oocytes were lysed with: (in mM) 150 NaCl, 0.2 phenylmethylsulfonyl fluoride, 20 NaF, 10 Na3VO4, 50 Tris (pH 7.4), and the following detergents: 1% Nonidet P-40, 1% CHAPS, 1% Triton X-100, and 0.5% SDS. Samples were clarified by two separate centrifugations at 10,000 × g for 20 min before the addition of standard SDS-PAGE loading buffer and separation by SDS-PAGE on 15% or 4-20% gradient gels. After transfer to polyvinylidene difluoride membrane, Western blots were performed with an in-house primary antibody raised by injection into rabbits of a mammalian expression vector expressing full-length hMiRP2. Detection was via goat anti-rabbit horseradish peroxidase-coupled secondary antibodies (Bio-Rad) for fluorography. Band intensities for assessment of RNAi efficiency at the protein level were quantified as for cDNA bands.

    RESULTS
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

A Xenopus MiRP Family Is Constitutively Expressed in Oocytes-- Xenopus EST data bases were searched with mammalian KCNE gene sequences, leading to the identification of four Xenopus clones designated here as xMinK, xMiRP2, xMiRP4.1, and xMiRP4.2 on the basis of their closest mammalian KCNE relatives. The four Xenopus genes were subsequently cloned from cDNA isolated by RT-PCR from stages V and VI Xenopus oocyte mRNA (Fig. 1). Homologues of mammalian MiRP1 and MiRP3 were not detected in Xenopus EST data bases. Mammalian MiRP2 co-assembles with several diverse Kv channel alpha  subunits, removing the voltage dependence of KCNQ1 (9), suppressing HERG current without altering gating kinetics (9, 11), and converting Kv3.4 to a subthreshold-activating delayed rectifier (7). Here we investigated the effects of the interaction between xMiRP2 and the cardiac-delayed rectifiers HERG and KCNQ1. Effects of human MiRP2 on Kv3.4 are pronounced in mammalian cells but not in Xenopus oocytes (7). This interaction was not covered here but is currently under study.


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Fig. 1.   An X. laevis MiRP family is expressed in oocytes. A, sequence alignment of Xenopus KCNE gene products and their human homologues shows predicted transmembrane domains (underlined), 100% conserved (*), and partially conserved residues (. :) using ClustalW 1.81 with default settings. B, unrooted dendrogram of evolutionary relationships between Xenopus and mammalian MiRP amino acid sequences using ClustalW 1.81 with default settings. C, PCR of Xenopus MinK, MiRP2, MiRP4.1, and MiRP4.2 cDNA after RT-PCR from oocyte mRNA. Arrow indicates 500-bp marker position after electrophoresis on ethidium bromide-stained 1% agarose gels.

Injected HERG and KCNQ1 Are Modulated by Overexpressed xMiRP2 in Oocytes-- Injection of HERG cRNA into oocytes resulted in the expression of rapidly inactivating delayed rectifier K+ currents that passed large tail currents during a -30-mV tail pulse because of rapid recovery from inactivation and slow deactivation. Co-injection of xMiRP2 cRNA with HERG in oocytes resulted in the complete suppression of HERG current (Fig. 2, A and B). The results demonstrate that xMiRP2 functions like hMiRP2 to strongly inhibit HERG current when it is overexpressed in Xenopus oocytes. Overexpression of xMiRP2 with KCNQ1 in oocytes resulted in an increase in the time- and voltage-independent component of KCNQ1 current as observed previously for human MiRP2 (Fig. 2, C and D). Xenopus MiRP2 can thus modulate human K+ channels when overexpressed in oocytes.


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Fig. 2.   Overexpressed xMiRP2 modulates HERG and KCNQ1 in oocytes. A, exemplar raw current traces generated from TEVC of oocytes injected with 5 ng of HERG or co-injected with 3 ng of xMiRP2 and 5 ng of HERG cRNA. Oocytes were held at -80 mV and stepped to voltages between -120 and +60 mV, with a -30-mV tail pulse (protocol inset). Dashed lines indicate zero current level. B, mean tail current-voltage relationships from traces as in panel A for HERG (solid squares) or xMiRP2/HERG (open squares) channels in oocytes, n = 8. Error bars indicate S.E. C, exemplar raw current traces generated from TEVC of oocytes injected with 10 ng of hKCNQ1 or co-injected with 3 ng of xMiRP2 and 10 ng of hKCNQ1 cRNA. Dashed lines indicate zero current level. Oocytes were held at -80 mV and stepped to voltages between -120 and +60 mV, with a -30-mV tail pulse (protocol inset). D, mean current-voltage relationships from traces as in panel C, for hKCNQ1 (solid squares) or xMiRP2/hKCNQ1 (open squares) channels in oocytes (n = 9-12). Error bars (S.E.) are smaller than point size.

Endogenous Oocyte xMiRP2 Is Suppressed by the Injection of siRNA Oligos-- To evaluate whether endogenous levels of xKCNE subunits are sufficient to modulate cloned mammalian Kv channel alpha  subunits expressed in oocytes, we tested the applicability in oocytes of the RNAi sequence-specific post-transcriptional silencing technique that has been shown to suppress gene expression in a variety of other cell types (30). A double-stranded xMiRP2-specific, siRNA oligo was injected into Xenopus oocytes, and the effects were compared against those of a scrambled control siRNA oligo. To ensure that xMiRP2 siRNA was causing a loss of xMiRP2 mRNA, RNA was isolated from oocytes injected with siRNA for xMiRP2, scrambled siRNA, or cRNA for xMiRP2. RT-PCR to produce cDNA was followed by normalization of samples to Xenopus beta -actin cDNA concentration (Fig. 3A, top panel). Next, normalized cDNA samples were probed for xMiRP2 cDNA by PCR using sequence-specific primers (Fig. 3A, middle panel). Compared with batches of oocytes injected with scrambled control siRNA, the band intensity of amplified xMiRP2 cDNA from batches of oocytes injected with xMiRP2 siRNA was reduced 8-fold as assessed by densitometry (Fig. 3A, bottom panel). Injection of 3 ng of xMiRP2 cRNA, in contrast, increased the band intensity of amplified xMiRP2 cRNA 30-fold (n = two batches of 8-12 oocytes). To assess the effects of xMiRP2 siRNA at the protein level, antibodies raised to full-length human KCNE3 and 80% similar to xMiRP2 in the transmembrane and C-terminal domains were used to probe injected oocyte lysates (Fig. 3B, upper panel). Probing the oocyte lysates with anti-MiRP2 antibody gave a band at 15-18 kDa, in the range expected for xMiRP2. Although xMiRP2 has one or more predicted N-glycosylation sites, depending on the prediction method used, only one band was visible here. We concluded therefore that the large majority of xMiRP2 protein present in the oocyte at baseline is in one particular glycosylation state, probably the mature form, as observed previously for mouse MiRP2 in the murine C2C12 skeletal muscle cell line (7). Similar levels of xMiRP2 protein were detected in water-injected oocytes and oocytes injected with scrambled control siRNA, whereas oocytes injected with xMiRP2 siRNA showed a 5-fold reduction of endogenous xMiRP2 protein as assessed by densitometry (n = two batches of oocytes) (Fig. 3B, lower panel). Thus, RNAi can be used in Xenopus oocytes to specifically suppress endogenous genes, in this case xMiRP2.


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Fig. 3.   Sequence-specific RNAi efficiently suppresses endogenous xMiRP2 in oocytes. A, semiquantitative PCR of cDNA generated by RT-PCR of mRNA isolated from Xenopus oocytes. Upper panel, Xenopus mRNA normalized to endogenous beta -actin gene. Left to right, oocytes injected with 500 pg of scrambled control siRNA, 500 pg of xMiRP2 siRNA, and 3 ng of positive control xMiRP2 cRNA. Bands were generated by PCR using primers specific for Xenopus beta -actin and indicate equal amounts of mRNA-generated cDNA loaded in each lane. Center panel, PCR from cDNA generated from Xenopus oocyte mRNA by RT-PCR and normalized to Xenopus beta -actin (see above). Left to right, oocytes injected with 500 pg of scrambled control siRNA (con siRNA), 500 pg of xMiRP2 siRNA (xM2 siRNA), or 3 ng of positive control xMiRP2 cRNA (xM2 cRNA). Bands were generated by PCR using primers specific for Xenopus MiRP2 and indicate specific post-transcriptional gene silencing of xMiRP2 by xMiRP2 siRNA. Lower panel, densitometry of xMiRP2 bands indicating an 8-fold reduction of xMiRP2 cDNA band intensity using xMiRP2 siRNA and a 30-fold increase using MiRP2 cRNA. B, upper panel, Western blot using KCNE3-specific antibodies from lysates of Xenopus oocytes injected with (left to right) water, 500 pg of xMiRP2 siRNA (xM2 siRNA), of 500 pg of scrambled control siRNA (con siRNA). Protein loaded per lane was normalized to total protein concentration (20 µg/lane) using the Bradford assay. Lines indicate 18 kDa (upper) and 6.4 kDa (lower). Lower panel, densitometry of xMiRP2 bands indicating a 5-fold reduction of xMiRP2 protein concentration using xMiRP2 siRNA and no significant reduction with scrambled control siRNA.

Endogenous Oocyte xMiRP2 Inhibits Heterologously Expressed HERG Currents-- The effects of the suppression of endogenous xMiRP2 expression on injected HERG currents were assessed next. HERG is an especially suitable indicator of endogenous xMiRP2 contribution to heterologously expressed K+ currents, because the unique gating attributes of HERG channels (i.e. rapid inactivation at positive voltages and rapid recovery from inactivation at negative voltages (31)) allow the assessment of small HERG tail currents at -30 mV, a voltage at which contamination from other oocyte currents and leaks is negligible. Varying amounts of HERG cRNA were injected into oocytes in the absence or presence of xMiRP2-specific siRNA to silence endogenous xMiRP2, and HERG tail currents were measured by TEVC after 3 days. At levels of HERG cRNA that normally expressed virtually zero HERG tail current (as much as 0.7 ng), co-injection with xMiRP2 siRNA rescued HERG currents to 500 nA as anticipated when an endogenous protein that suppresses HERG is silenced (Fig. 4, A and B). Indeed, xMiRP2 silencing increased HERG currents at all concentrations tested below 5 ng of HERG (Fig. 4C). This effect was specific because scrambled control siRNA had no effect (Fig. 4D). To eliminate the possibility that xMiRP2 siRNA oligos increased HERG current because of an artifact via direct interaction with HERG protein rather than by post-transcriptional xMiRP2 gene silencing, we switched expression systems and assessed the effects of xMiRP2 siRNA on HERG currents in CHO cells using a whole-cell voltage clamp. xMiRP2 siRNA should have no effect on HERG current density in CHO cells, because even if CHO cells express endogenous hamster MiRP2, xMiRP2 siRNA is sequence-specific for Xenopus MiRP2. Transfection of cells with xMiRP2, siRNA, and HERG cDNA produced a mean current density not significantly different from cells transfected with HERG cDNA alone. This result argues against our previous oocyte results being an artifact caused by direct interaction of xMiRP2 siRNA with HERG protein (Fig. 4E).


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Fig. 4.   Endogenous xMiRP2 inhibits heterologous HERG current in oocytes. A, exemplar current families recorded between -120 and +60 mV from oocytes injected with 0.7 ng of HERG cRNA or co-injected with 0.7 ng of HERG cRNA and 500 pg of xMiRP2 siRNA (xM2 siRNA). Dashed lines indicate zero current level. Oocytes were held at -80 mV and then stepped to voltages between -120 and +60 mV with a -30-mV tail pulse (protocol inset). B, shown is the mean tail current/voltage relationship from oocytes injected with 0.7 ng of HERG cRNA (solid squares) or co-injected with 0.7 ng of HERG cRNA and 500 pg of xMiRP2 siRNA (open squares), protocol as in A, n = 14-17. Error bars indicate S.E. C, HERG cRNA dose response. Peak tail currents from oocytes injected with various concentrations of HERG cRNA with or without 500 pg of xMiRP2 siRNA using the protocol in A (inset), n = 10-17 oocytes/point. Points were joined arbitrarily with straight lines. D, mean peak tail currents recorded using the protocol in A from oocytes injected with 0.5 or 1 ng of HERG cRNA with or without 500 pg scrambled control siRNA (con siRNA), n = 7-11 per condition. No significant differences were observed between current densities recorded on the same day (p > 0.05, unpaired Student's t test). Error bars indicate S.E. E, mean peak tail current densities recorded using a protocol similar to that shown in A (inset) from CHO cells transfected with HERG cDNA or co-transfected with HERG cDNA and xMiRP2 siRNA; n = 8-10. The current was normalized to cell capacitance. No significant differences were observed between current densities (p > 0.05, unpaired Student's t test). Error bars indicate S.E.

Variability in Effects of hMiRP1 on HERG Suggests Rescue from Inhibition by Endogenous xMiRP2-- In contrast to human and Xenopus MiRP2, which completely suppress HERG currents expressed in oocytes (9) (presumably by inhibiting functional HERG channel formation), hMiRP1 partially reduces HERG currents by the formation of lower conductance fast deactivating functional channel complexes (6). The variability of hMiRP1 effects on HERG currents depending on expression system or HERG expression level has been reported previously. Although hMiRP1 mutations have been linked to inherited and acquired arrhythmia in three studies, and the effects of hMiRP1 on HERG have been noted by several groups, the reported effects differ (6, 11, 13-18). We hypothesized that some of the variability of the effects of hMiRP1 on HERG results from the displacement of xMiRP2 and that addition of hMiRP1 cRNA would rescue HERG currents at lower HERG levels (at which HERG is normally inhibited by endogenous xMiRP2) by forming functional hMiRP1/HERG complexes in favor of nonfunctional xMiRP2/HERG complexes.

We first recapitulated previous reports of hMiRP1 modulation of HERG in both Xenopus oocytes and CHO cells. Using 10 ng of HERG cRNA/oocyte to achieve a high HERG current density, we compared current attributes with and without the injection of hMiRP1 cRNA. Co-injection of either 10 or 20 ng of hMiRP1 cRNA/oocyte resulted in similar ~60% reductions in HERG tail currents (Fig. 5A, left panel). hMiRP1 also reduced HERG currents in CHO cells, producing an ~40% reduction in tail current density compared with HERG co-transfected with blank plasmid (Fig. 5A, right panel). Next, similar experiments were conducted using lower concentrations of HERG cRNA in oocyte expression studies. In contrast to the high current density HERG experiments, co-injection of hMiRP1 cRNA (2 ng/oocyte) with lower levels of HERG cRNA (0.5 ng/oocyte) actually up-regulated HERG -30-mV tail currents ~3-fold after 2 and 3 days of expression (Fig. 5B). Similar effects were observed with xMiRP2 siRNA or a combination of hMiRP1 cRNA and xMiRP2 siRNA in the same batch of oocytes (Fig. 5B). The results suggest that, paradoxically, because hMiRP1 is able to couple with HERG and prevent suppression by endogenous levels of xMiRP2, at low HERG levels hMiRP1 increases HERG currents despite the formation of channels with lower unitary conductance.


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Fig. 5.   Endogenous xMiRP2 mediates density-dependent variation in modulation of HERG by human MiRP1. A, left panel, mean tail current densities recorded at -120 mV using TEVC of oocytes after injection with 10 ng of HERG or 10 ng of HERG cRNA co-injected with 10 or 20 ng of hMiRP1 cRNA as indicated, n = 5-9. Error bars indicate S.E.; asterisk for HERG-alone currents indicates a significant difference from other currents (p < 0.05, unpaired Student's t test). Right panel, mean tail current densities recorded at -30 mV using whole-cell patch clamp of CHO cells co-transfected with 3 µg of HERG cDNA and either 2 µg of hMiRP1 cDNA or blank plasmid, with current normalized to cell capacitance; n = 8-12. Error bars indicate S.E., asterisk for HERG-alone currents indicates significant difference from other currents (p < 0.05, unpaired Student's t test) B, mean tail current densities recorded at -30 mV using TEVC of oocytes injected with 0.5 ng of HERG cRNA alone or with either 2 ng of hMiRP1 or 500 pg of xMiRP2 siRNA, or both; n = 5 oocytes (day 2) and 10 oocytes (day 3)/condition. Asterisks for HERG-alone currents indicate significant differences from other currents on same day (p < 0.01, unpaired Student's t test).


    DISCUSSION
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Previously, the finding that the KCNQ1 alpha  subunit is expressed endogenously in X. laevis oocytes and up-regulated by the heterologous expression of mammalian MinK led to the discovery that human cardiac IKs is formed by MinK/KCNQ1 complexes in vivo (10). Here we demonstrate that Xenopus oocytes also express relatives of mammalian MiRPs and that these endogenous xMiRPs can interact with injected mammalian Kv channel alpha  subunits. HERG, like KCNQ1, is a human cardiac Kv channel widely studied because of the role it plays in cardiac repolarization and the propensity of HERG to interact nonspecifically with a wide range of therapeutic agents, a propensity that contributes to acquired cardiac arrhythmia (21, 32). The functional properties of HERG are modified by mammalian MinK, MiRP1, and MiRP2 (6, 9, 11, 14, 18, 29). We have demonstrated that endogenous levels of xMiRP2 are sufficient to modify overexpressed HERG in oocytes. HERG currents recorded after the injection of HERG cRNA into oocytes are thus the sum product of "pure" HERG channels, xMiRP2-suppressed HERG, and/or channels formed by HERG with other xMiRP subunits. This fact can dramatically affect the interpretation of previous and future studies on HERG gating, pharmacology, and trafficking. We have yet to elucidate the mechanism by which xMiRP2 inhibits HERG, but this complete suppression suggests the formation of a non-functional channel, perhaps one that fails even to reach the plasma membrane. A question then remains to be answered. What happens to MiRP2-sequestered HERG, and can it be rescued by stimulating regulatory pathways or with use of drugs that bind to HERG and that rescue HERG channels containing trafficking mutations associated with long QT syndrome (33, 34)? If so, the association of endogenous xMiRP2 with HERG may impact previous and future oocyte-based studies of HERG regulation and pharmacological rescue. The hMiRP1 versus xMiRP2 data presented here suggest that endogenous xMiRP2 forms the molecular basis for some of the previously reported variability in hMiRP1/HERG oocyte studies. The hMiRP1 versus xMiRP2 findings also highlight the importance of understanding the molecular background of expression systems used in alpha -beta subunit interaction studies. We anticipate that this phenomenon (i.e. the functional effects of an introduced mammalian beta  subunit resulting partially from the displacement of an endogenous expression system beta  subunit) will prove to be a recurring theme once the full range of Kv channel interactions with Xenopus and mammalian MiRPs is explored in oocytes and in other cell types.

The issue of MiRP/alpha -subunit interaction is particularly contentious when cardiac Kv channels are considered. Both HERG and KCNQ1, the alpha  subunits that generate the two principal repolarization currents, IKr and IKs, respectively, in human ventricular myocardium, are modulated by MinK, MiRP1, and MiRP2 in at least one expression system (35). KCNQ1 is also strongly suppressed by MiRP3 (KCNE4) (36) and forms with MiRP4 a slowly activating channel that resembles MinK/KCNQ1 channels but with a much more positive voltage dependence of activation (37). Little doubt exists that MinK/KCNQ1 complexes form human cardiac IKs. Compared with currents formed by overexpression of KCNQ1 alone, MinK/KCNQ1 channels exhibit a 4-fold higher conductance and a greatly slowed activation rate, generating a current that strongly resembles IKs in terms of biophysical attributes, regulation, and pharmacology. Further, mutations in both subunits that diminish channel function are associated with long QT syndrome in humans (19, 23, 24, 38-40). More debate exists concerning the precise molecular correlate of human IKr. Although the introduction of HERG alpha  subunits into oocytes or immortal mammalian cell lines creates a potassium current with many of the attributes of cardiac IKr (21, 32), coexpression with human MiRP1 alters the single channel properties of HERG to produce a current more closely resembling previous in vivo recordings of IKr (6). Mutations in hMiRP1 diminish flux through hMiRP1/HERG channels and are associated with inherited long QT syndrome (6, 17). Further, some hMiRP1 mutations increase sensitivity to the blockade of hMiRP1/HERG channels in vitro by drugs known to block IKr (e.g. clarithromycin). Importantly, in each case the same drug (e.g. sulfamethoxazole) had precipitated drug-induced torsades de pointes and a prolonged QTc interval, specifically in patients from whom each mutation was isolated (6, 15). However, largely as a result of the reported wide variability in effects of MiRP1/HERG coexpression, the assignment of this combination of subunits as the molecular basis of human IKr is still questioned. Here we have noted one cause of the reported variability in hMiRP1/HERG behavior: the dependence of hMiRP1 effects on HERG expression level because of interference from endogenous MiRPs such as xMiRP2. At low levels of HERG cRNA, HERG current is completely suppressed by endogenous xMiRP2. The fact that we were able to subsequently reconstitute HERG current by either silencing the expression of xMiRP2 or introducing an ostensibly preferred partner illustrates just how promiscuous the association between an alpha  subunit and the MiRPs can be. Can we infer from this that HERG will favor in every cell type an interaction with MiRP1 over other beta  subunits? Enough evidence does not exist at this time to support such a claim. However, our findings in conjunction with those from other studies highlight the important idea that native currents such as IKr are probably formed from a spectrum of molecular components with temporal and spatial dependence, and thus the success of direct native-cloned current comparisons is governed not only by the choice of expression system but also by the inclusion of possible beta  subunit partners and by the precise choice of native tissue source. Increasingly, for example, evidence suggests that IKr is regulated by MinK in some cardiac myocytes: MinK up-regulates HERG in some mammalian cell lines, knockdown of MinK suppresses HERG in murine myocytes, and immunoprecipitation of MinK from horse heart pulls down HERG, and KCNQ1 (25, 29, 41, 42).

Oocytes remain an expression system with numerous advantages for the assessment of ion channel function. Here we have described the first reported use (to our knowledge) of RNAi post-transcriptional gene silencing in the oocyte system. The use of RNAi to control endogenous modifying subunits in oocytes, mammalian cell expression systems (which may also contain endogenous MiRPs), and native studies will improve our understanding of the heteromultimeric molecular basis of human Kv currents and also allow us to accurately define the properties of "pure" alpha  subunit complexes. This knowledge will facilitate the linkage of channel alpha  and beta  subunit genes to inherited and acquired disease, the rational design of channel-directed therapeutic drugs, and screening for avoidance of unwanted channel-drug interactions. Consideration of endogenous modifying subunits in heterologous expression systems is likely to be most crucial when undertaking single channel recordings, because the lower alpha  subunit expression levels often required for these studies (when compared with macroscopic studies) will generate a higher molar ratio of endogenous beta  subunits to exogenous alpha  subunits. The impact of endogenous xMiRPs on macroscopic studies should not be overlooked, however, because our results here show that endogenous xMiRP2 affects the behavior of HERG, for example, at tail current densities in the range widely used by investigators of whole-cell HERG biophysics, pharmacology, and intersubunit interactions (6, 11, 43, 44).

    ACKNOWLEDGEMENT

We are grateful to D. Christini for critical reading of the manuscript.

    FOOTNOTES

* This work was supported by grants from the Greenberg Atrial Fibrillation Fund and the American Heart Association (to G. W. A. and Z. A. M.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AF545500, AF545501, and AF545502, for X. laevis MinK, MiRP2, and MiRP4.1, respectively.

Dagger These authors contributed equally to this work.

** To whom correspondence should be addressed: Weill Medical College of Cornell University, 520 E. 70th St., Starr 463, New York, NY 10021. Tel.: 212-746-6275; Fax: 212-746-7984; E-mail: gwa2001@med.cornell.edu.

Published, JBC Papers in Press, January 15, 2003, DOI 10.1074/jbc.M212751200

    ABBREVIATIONS

The abbreviations used are: Kv channel, voltage-gated potassium channel; MiRP, MinK-related peptide; hMiRP, human MiRP; CHO, Chinese hamster ovary; RNAi, RNA interference; siRNA, short interfering RNA; TEVC, two-electrode voltage clamp; IKs, slow activating potassium current; IKr, rapidly activating potassium current; Ito, transient outward current; RT, reverse transcriptase; EST, expressed sequence tag; CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid.

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
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