From the
Department of Pathology, the University of Alabama, Birmingham, Alabama 35294 and the ¶Department of Molecular Genetics, The University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030
Received for publication, November 27, 2002 , and in revised form, March 25, 2003.
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ABSTRACT |
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INTRODUCTION |
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Tartrate-resistant acid phosphatase (TRAP) is an iron-binding protein that is abundantly expressed in mature osteoclasts (5, 6, 7). TRAP has been shown to play an important role in bone resorption. In vitro studies demonstrated that a neutralizing antibody against TRAP inhibited bone resorption (8). Confirming the in vitro data, mice lacking TRAP exhibited a defect in endochondral ossification and a mild osteopetrosis (9). Conversely, transgenic mice overexpressing TRAP resulted in a decrease in trabecular bone density with characteristic mild osteoporosis (10). Recent studies have suggested that TRAP regulates bone resorption by mediating the degradation of endocytosed matrix products during transcytosis in activated osteoclasts (5, 11, 12).
TRAP expression is often undetectable in osteoclast precursors, but its expression is dramatically up-regulated during osteoclast differentiation (7). As a result, TRAP has been widely used as a marker for osteoclasts (2). Since the discovery of RANKL, it has been established that RANKL plays a key role in TRAP expression during osteoclast differentiation (13, 14). However, the molecular mechanism by which RANKL regulates TRAP expression during osteoclast differentiation still remains unknown.
RANKL, identified as a member of the TNF superfamily (13, 15, 16, 17), is a potent activator of osteoclast differentiation, function, and survival (18, 19). RANKL exerts its effects by binding to its receptor RANK, which was identified as a member of the TNF receptor family (16). Upon RANKL binding, RANK interacts with various TNF receptor-associated factors to initiate intracellular signaling pathways leading to the activation of two transcription factors: NF-B and AP-1 (18, 20, 21). Both NF-
B and AP-1 play an essential role in osteoclast differentiation and function (22, 23).
Murine TRAP promoter was previously cloned and characterized (24, 25, 26). Importantly, a 1.8-kb mouse promoter region is capable of driving the osteoclast-specific expression of Src gene fused downstream of the TRAP promoter, in transgenic mice (27), indicating that this 1.8-kb promoter contains important regulatory elements required for transcriptional activation in osteoclasts. In our present study, we investigated the molecular mechanism underlying the RANKL-dependent TRAP expression during osteoclast differentiation by using a luciferase reporter construct containing the 1.8-kb TRAP promoter. We identified a 12-bp sequence in the TRAP promoter that is involved in TRAP transcriptional activation in response to RANKL. Interestingly, we also showed that this 12-bp sequence regulates the RANKL-induced TRAP gene transcription by using upstream stimulating factors (USF) 1 and 2, instead of NF-B and AP-1 that are known transcription factors activated by RANKL. These data not only establish a functional role of USF1 and USF2 in TRAP expression in osteoclasts but also define a new transcriptional mechanism by which RANKL regulates gene transcription.
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EXPERIMENTAL PROCEDURES |
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Construction of a TRAP Promoter-Luciferase Reporter Plasmid and Deletion MutantsA 1858-bp mouse TRAP promoter (from 1858 to 1, in relation to ATG) was amplified by PCR using primers derived from published sequences (24, 25, 26). The forward primer (5'-cgggatcccccgggtctcccttaactcctgggac-3') contains a BamHI site (underlined) at its 5'-end, whereas the reverse primer (5'-cgaagctttgtgaggaagagagggagttcagag-3') has a HindIII site (underlined) at its 5'-end. The PCR was performed using genomic DNA from C3H mice from Harlan Industries (Indianapolis, IN) as a template and high fidelity Pfx polymerase from Invitrogen (Carlsbad, CA). PCR products were digested with BamHI and HindIII and subcloned into a luciferase reporter plasmid pGL3-basic (Promega, Madison, MI) between BglII and HindIII. Cohesive ends resulting from BamHI and BglII digestion are compatible. The amplified TRAP promoter was confirmed by sequencing, and the resulting reporter plasmid was named TP(1858).
9 deletion mutants of TP(1858), TP(1239), TP(1199), TP(1159), TP(1119), TP(1079), TP(1039), TP(839), TP(639), and TP(439), were prepared using forward primers derived from different 5' positions (the numbers in parentheses) of the TRAP promoter and the same reverse primer as used above for amplifying the longest construct TP(1858). All of these forward primers contain a BamHI site at their 5'-ends. PCR was performed using TP(1858) as a template and high fidelity Pfx polymerase from Invitrogen. The amplified TRAP promoter fragments were subcloned into pGL3-basic (Promega) and their sequence was confirmed by sequencing.
Cell Cultures and Transient TransfectionsRAW 264.7 cells (ATCC, Manassas, VA) were cultured in Dulbecco's modified Eagle's medium containing 10% heat-inactivated fetal bovine serum in tissue culture plates and passed by lifting the cells by scraping. Cells were transiently transfected using LipofectAMINE Plus transfection reagents from Invitrogen. For transfections, cells were plated in six-well cell culture plates at the concentration of 8 x 105 cells/well 1 day before transfections. For each well, 2 µg of reporter plasmid plus 0.05 µg of internal-control plasmid phRL-SV40 (Promega) were used. Transfected cells were treated with or without 200 ng/ml GST-RANKL for various times after transfection and lysed for luciferase assays using the Dual-Luciferase Reporter Assay System from Promega.
Nuclear Extract PreparationBone marrow macrophages (BMMs) were isolated from long bones of 4- to 8-week-old C3H mice from Harlan Industries as described previously (28) and cultured in -minimal essential medium containing 10% heat-inactivated fetal bovine serum in the presence of 10 ng/ml recombinant M-CSF (R&D Systems, Minneapolis, MN). RAW 164.7 cells were cultured as described above in tissue culture dishes. Cells were treated with or without RANKL treatment for the indicated times. Upon confluence (about 1 x 107 cells), cells were washed with cold PBS three times. Cell were then scraped off the dishes, spun down, resuspended in 1.5 ml of cold PBS, and transferred to 2-ml microcentrifuge tubes. Cells were pelleted in a microcentrifuge for 30 s, media were removed, and the cells were resuspended in 500 µl of hypotonic lysis buffer (10 mM Hepes-KOH, pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.5 mM DTT, 0.5 mM phenylmethylsulfonyl fluoride; DTT and phenylmethylsulfonyl fluoride were added freshly). Cells were lysed for 15 min on ice, at which time 32 µl of 10% Nonidet P-40 was added to the suspension, followed by vortexing the tube for 15 s and incubating on ice for 10 min. Nuclei were spun down and resuspended in 100 µl of nuclear extraction buffer (20 mM Hepes-KOH, pH 7.9, 420 mM NaCl, 1.2 mM MgCl2, 0.2 mM EDTA, 25% glycerol, 0.5 mM DTT, 0.5 mM phenylmethylsulfonyl fluoride, 0.5 mM AEBSF, 5 µg/ml pepstatin, and 5 µg/ml leupeptin; DTT, phenylmethylsulfonyl fluoride, BAESF, pepstatin, and leupeptin were added freshly to the buffer). The nuclei were incubated with the extraction buffer on ice for 20 min and spun down in a microcentrifuge. The supernatant (nuclear extract) was aliquoted, quickly frozen in dry ice/ethanol bath, and stored at 80 °C. Protein concentration of nuclear extracts was determined using Bio-Rad protein assay kit (Bio-Rad, Hercules, CA).
Electrophoretic Mobility Shift AssaysOligonucleotides used for EMSAs were end-labeled with 32P by T4 polynucleotide kinase (Invitrogen). 25 x 104 cpm probe was incubated with 3 µg of nuclear extracts in a 20-µl volume of binding reaction (10 mM Tris-Cl, pH 7.5, 100 mM NaCl, 10% glycerol, 50 ng/ml poly(dI·dC) on ice for 20 min). In competition experiments, a 50x and/or 100x excess amount of unlabeled competitors was premixed with labeled probe before being added to the binding mixture. The binding reaction was then allowed to proceed for 20 min on ice. In supershift experiments, probe was incubated with 3 µg/ml nuclear extracts in a 20-µl volume of binding reaction for 20 min on ice, at which time 4 µg of control IgG or 4 µg of specific antibodies were added, followed by incubation on ice for an additional 30 min. All binding mixtures were separated, using 0.5x TBE buffer as the running buffer, at 4 °C and 100 V for 3.5 h by 420% gradient TBE gels (Invitrogen) in a Novex Xcell II minicell electrophoresis system. The gels were transferred to 3M blotting paper and dried, and exposures were made to film.
In Vitro TranslationsIn vitro translated USF1 and USF2 were prepared by using a PROTEINscriptTM II-linked transcription/translation kit (Ambion, Austin, TX) and expression plasmids for USF1 (psvUSF1) and USF2 (psvUSF2) described in a previous study (29). These expression plasmids were constructed using vector pSG5 (Stratagene, La Jolla, CA), which contains the T7 promoter suitable for in vitro translations. Thus, PROTEINscriptTM II T7 was used for in vitro translation assays with these expression vectors. Briefly, 0.5 µg of each plasmid (pSG5, psvUSF1, or psvUSF2) was used to set up transcription reactions (10 µl of reaction volume for each experiment) following the protocol provided by the manufacturer. Subsequently, 2 µlof the transcription reaction was used for the translation reaction following the protocol provided by the manufacturer (50 µl of reaction volume for each experiment). 5 µl from each translation reaction was then used to perform EMSA as described above.
Site-directed MutagenesisPoint mutations were introduced in the context of the longest TRAP promoter construct TP(1858) using a QuikChangeTM site-directed mutagenesis kit (Stratagene, La Jolla, CA). Oligonucleotides used to mutate the USF-binding site were 5'-GCTACAGCCAGCCACGgaGTGTGTGCCTTCTGG-3' and 5'-CCAGAAGGCACACACtcCGTGGCTGGCTGTAGC-3. These oligonucleotides were purified by polyacrylamide gel electrophoresis. PCRs were performed in a 50-µl volume with Pfu polymerase, 10 ng of DNA template TP(1858), and 125 ng of each oligonucleotide using the following conditions: 95 °C for 30 s, 1 cycle; 95 °C for 30 s, 55 °C for 1 min, and 68 °C for 12 min, 18 cycles; and 4 °C park. The PCR products were treated with DpnI (10 units) for 60 min at 37 °C. XL1-blue supercompetent cells were transformed with the DpnI-treated PCR mixture as described in the manufacturer's instruction manual and plated on ampicillin plates. Plasmids were prepared from individual colonies and sequenced to confirm the correctness of introduced mutations.
Sequence AnalysisSequence analysis was performed using the Genetic Computer Group (Madison, WI) sequence analysis software.
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RESULTS |
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The amplified TRAP promoter was subcloned into pGL3-basic in sense orientation to the luciferase gene to generate a reporter construct TP(1858). Because primary BMMs are extremely difficult to transfect, we used RAW264.7 cells for our transfection assays. TP(1858) was transiently transfected into RAW264.7 cells, and the transfection result shows that RANKL activated the TRAP promoter in a time-dependent manner with a maximal induction at day 4 (about 2.5-fold) (Fig. 1B), thus indicating that the 1858-bp TRAP promoter contains one or more sequences mediating RANKL-induced activation of TRAP gene transcription. In addition, because the data show that a 4-day RANKL treatment gave rise to the highest TRAP promoter activation, all of our following transfection studies will be performed with a 4-day RANKL treatment.
Localization of a 40-bp TRAP Promoter Region (1239 to 1199) That Binds RANKL-induced Nuclear ProteinsTo characterize the sequence(s) involved in the RANKL-induced TRAP transcription, we generated five deletion mutants of the TRAP promoter: TP(1239), TP(1039), TP(839), TP(639), and TP(439) (Fig. 2A). These mutants contain RANKL promoter regions starting from different 5' positions (the numbers in parentheses) and ending at the same 3' site (1). We then used these deletion mutants to perform transfection studies (Fig. 2B). Although our longest construct TP(1858) gave rise to an increase of about 2.5-fold in TRAP promoter activity in response to RANKL, deletion of a 619-bp region (1858 to 1239) only resulted in an induction of about 1.6-fold (Fig. 2B), indicating that this 619-bp region plays a role in RANKL-dependent TRAP gene transcription. Moreover, deletion of another 200-bp region (1239 to 1039) totally abolished RANKL responsiveness (Fig. 2B), revealing that this 200-bp region is also critical for RANKL-dependent TRAP gene transcription. Taken together, these data show that two TRAP promoter regions are involved in RANKL-dependent TRAP transcription.
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In this study, we chose to focus on characterizing the cis-element(s) in the 200-bp region (1239 and 1039). To this end, we first determined whether the 200-bp region binds any nuclear proteins in response to RANKL. We synthesized five overlapping oligonucleotides spanning the entire 200-bp TRAP promoter region (Fig. 2C). We labeled these oligonucleotides and used them as probes to perform EMSA with nuclear extracts from untreated and RANKL-treated RAW264.7 cells (Fig. 2D). Oligonucleotide I gave rise to a major band (band A) in EMSA with untreated RAW264.7 cells (lane 2). This band was significantly enhanced when RANKL-treated RAW264.7 nuclear extracts were used (lane 3), suggesting that nuclear proteins corresponding to band A may be implicated in RANKL-induced TRAP transcription. In addition to band A, a minor band with a higher mobility (band B) was also observed (lanes 2 and 3). However, in our subsequent EMSAs, band B appears to be very unstable. Thus, we only focused on band A in the present study. Interestingly, oligonucleotide IV also binds nuclear proteins induced by RANKL in our EMSA (lanes 11 and 12). These data suggest that oligonucleotides I and IV may contain cis-elements regulating RANKL-induced TRAP transcription.
The 40-bp TRAP Promoter Region (1239 to 1199) Mediates RANKL-induced TRAP Transcription via Transcription Factors Other Than NF-B and AP-1Next, we prepared four additional deletion mutants in which a 40-bp promoter region was deleted in a progressive fashion starting from 1239 (Fig. 3A). Consistent with the EMSA above (Fig. 2D), our transfection studies with these mutants indicate that the 40-bp region from 1239 to 1199 is critical for RANKL-induced TRAP promoter activation (Fig. 3B), confirming that oligonucleotide I contains one or more cis-elements regulating RANKL-induced TRAP transcription.
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The previous studies have well established that RANKL activates two transcription factors NF-B and AP-1 (18, 20, 21), which play a pivotal role in osteoclast differentiation (22, 23). However, our computer analysis found no consensus sequences for NF-
B or AP-1 in this 40-bp region, suggesting that the nuclear proteins binding to this 40-bp sequence are not NF-
B or AP1. To experimentally confirm this, we performed a competition assay using excess cold oligonucleotide I and oligonucleotides containing NF-
B/AP-1 consensus sequences as competitors (Fig. 4A). Although excess cold oligonucleotide I was able to compete for the nuclear protein binding, both NF-
B and AP-1 oligonucleotides failed to do so, further suggesting that this 40-bp does not bind NF-
B or AP-1. Subsequently, our supershift assays with antibodies against p50, p65, c-Fos, and c-Jun confirmed that the 40-bp sequence binds transcription factors other than NF-
B and AP-1 (Fig. 4B).
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Identification of a 12-bp Sequence, AGCCACGTGGTG, That Specifically Binds RANKL-induced USF1 and USF2 in Oligonucleotide IUpon the exclusion of NF-B and AP-1 as the nuclear proteins binding to oligonucleotide I, we proceeded to elucidate the identity of the nuclear proteins. Our computer analysis revealed that this 40-bp region contains putative binding sequences for many transcription factors, including ELP/SF1/FTZ (33), USF (34, 35), PPAR (36), CP1/2 (37), PHO4 (38, 39), and c-Myc (40). Because this information is not specific enough, we decided to identify the specific nuclear protein-binding sequence as our first step in elucidating the nuclear proteins. In doing so, we performed a series of competition assays using shortened oligonucleotides derived from oligonucleotide I as competitors (Fig. 5A). As shown in Fig. 5B, although SOI 1 competed efficiently for the nuclear protein binding (lane 2), SOIs 25 failed to do so (lanes 36), revealing the 5'-end of the specific nuclear protein-binding sequence (Fig. 5A). Similarly, the experiment in Fig. 5C showed that SOIs 611 could compete efficiently (lanes 27) but not SOI 12 (lane 8), elucidating the 3'-end of the sequence (Fig. 5A). Together, these data identified a 12-bp sequence within the 40-bp region that binds specifically the nuclear proteins induced by RANKL (Fig. 5A).
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The 12-bp sequence contains a sequence, CACGTG, that is recognized as a core sequence present in binding sites for both the Myc family members (40) and USF proteins (34, 35). This suggests that the nuclear proteins binding to the 12-bp sequence might be members of the Myc family or USF proteins. As a result, we performed supershift assays with antibodies against c-Myc, c-Max, USF1, and USF2 (Fig. 6A). Although antibodies against c-Myc and c-Max had no effect on band A (lanes 3 and 4), those against USF1 and USF2 supershifted the band (lanes 5 and 6), confirming that the nuclear proteins binding to the 12-bp sequence are USF1 and USF2. Moreover, USF1 antibody only partially supershifted band A, resulting in a weak band (lane 5). In contrast, USF2 antibody completely supershifted the band (lane 6). These data further indicate that this 12-bp sequence is able to bind USF proteins from RAW264.7 cells as either USF1·USF2 heterodimers or USF2 homodimers.
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At this point we identified a 12-bp sequence in TRAP promoter that binds USF1 and USF2 from RAW264.7. Because TRAP gene expression is also activated by RANKL in primary BMMs, we examined whether this sequence also binds RANKL-induced USF1 and USF2 from primary BMMs. As shown in Fig. 6B, oligonucleotide I binds nuclear proteins from untreated primary BMMs, resulting in two bands (A and B, lane 1). Band A is significantly enhanced when nuclear extracts from RANKL-treated BMMs were used (lane 2). Consistently, the pattern of banding in the EMSA (Fig. 6B) is very similar to that seen in the EMSA with nuclear extracts from RAW264.7 cells (Fig. 2D, lanes 2 and 3). Importantly, supershift assays indicate that the nuclear proteins binding to oligonucleotide I (band A) from primary BMMs treated with RANKL are also USF1 and USF2 (Fig. 6C). These results further support that the 12-bp sequence in TRAP promoter may play a functional role in RANKL-dependent TRAP transcription by utilizing USF1 and USF2.
As additional evidence supporting that this 12-bp sequence binds USF1 and USF2, we performed EMSA using in vitro translated USF 1 and USF2, which were prepared by using expression vectors psvUSF1 (for USF1) and psvUSF2 (for USF2) as described previously (29). These expression plasmids were constructed using vector pSG5 (29). As shown in Fig. 7, oligonucleotide I does not bind any proteins from in vitro translation reaction with control vector pSG5 in EMSA (lane 1). In contrast, oligonucleotide I binds proteins from in vitro translation reactions with psvUSF1 (lane 2) or psvUSF2 (lane 3). As expected, oligonucleotide I binds both USF1 and USF2 when both in vitro translated USF1 and USF2 were used in the EMSA (lane 4). Notably, 5 µlofthe in vitro translation reaction with psvUSF2 gave rise to much weaker band (lane 3) than that obtained with the same volume of the translation reaction with psvUSF1 (lane 2). This assay was independently repeated twice, and the same result was obtained. This discrepancy may result from a difference in the affinities of these in vitro translated USF proteins for the 12-bp sequence or from the low translation efficiency associated with psvUSF2. Nevertheless, these data strongly support that the 12-bp sequence is a USF-binding site.
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The 12-bp USF Binding Sequence Is Functionally Involved in the RANKL-induced TRAP TranscriptionFinally we determined whether the 12-bp USF-binding sequence is functionally involved in the RANKL-dependent TRAP transcription. To this end, we need to identify a mutation in the USF-binding site that is capable of blocking the USF binding. We synthesized a mutant oligonucleotide I in which TG, in the core binding CACGTG, were converted to GA (Fig. 8A). Competition assays show that mutant oligonucleotide I failed to compete for the USF binding (lane 3, Fig. 8B), indicating that the chosen mutation is sufficient to eliminate the binding capacity of the sequence. To exclude the possibility that the chosen mutation, although it abolish USF recognition, renders the sequence capable of associating with other nuclear proteins, mutant oligonucleotide I was used as probe to perform an EMSA (Fig. 8C). The data confirm that mutant oligonucleotide I did not bind any additional nuclear proteins.
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We introduced the same mutation in our longest reporter construct TP(1858), resulting in a reporter construct named mTP(1858) (Fig. 8D). In transfected RAW264.7 cells, although TP(1858) resulted in 2.5-fold induction in response to RANKL, mTP(1858) gave rise to only a 1.8-fold induction (p < 0.01) (Fig. 8E), indicating that the 12-bp USF binding sequence is functionally involved in RANKL-induced transcriptional activation of the TRAP gene.
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DISCUSSION |
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Our studies took advantage of a macrophage-like cell line RAW264.7, which is not only transfectable but also capable of differentiating into osteoclasts in response to RANKL (14). Significantly, our initial transfection studies showed that two distinct TRAP promoter regions are important for TRAP transcriptional activation in response to RANKL, suggesting that multiple transcriptional events are involved in RANKL-induced TRAP gene activation. A detailed characterization of one such region in this report revealed that a 12-bp sequence AGCCACGTGGTG is involved in enhancing TRAP gene transcription in response to RANKL. More significantly, we further showed that this 12-bp sequence does so by binding USF1 and USF2.
Transcription factors USF1 and USF2 were originally identified by their ability to bind to the adenovirus major late promoter (42). Structurally, USF1 and USF2 are related to the Myc family of transcription factors, which are characterized by the presence of a C-terminal basic helix-loop-helix-leucine zipper (bHLH-Zip) domain (33, 43). USF1, USF2, and the Myc family members were shown to recognize DNA sequences containing a core sequence CACGTG (29). Although the RANKL-responsive sequence (AGCCACGTGGTG) identified in TRAP promoter contains such a core sequence (underlined), our data showed that it binds USF1 and USF2 but not c-Myc or Max in response to RANKL (Fig. 6, A and C). This indicates that this 12-bp RANKL-responsive sequence regulates RANKL-induced TRAP transcription exclusively by utilizing USF1 and USF2. Notably, it was recently demonstrated that RANKL enhances significantly c-Myc expression in RAW264.7 cells during osteoclast differentiation (14). However, the precise role of c-Myc in TRAP transcription is controversial. Overexpression of a dominant negative c-Myc mutant in RAW264.7 cells resulted in a reduction in the levels of TRAP transcripts (14), supporting a positive role of c-Myc in TRAP expression. In contrast, c-Myc was recently shown to have a negative effect on TRAP transcription when overexpressed in a murine macrophage cell line P388D1 (44).
It has been previously shown that various other transcription factors are implicated in regulating TRAP transcription in osteoclasts. Particularly, the microphthalmia transcription factor MITF, a critical regulator of osteoclast function (45), plays an important role in controlling TRAP transcription (46). Similar to USF1 and USF2, MITF is also characterized as a bHLH-Zip protein, and it regulates TRAP transcription by binding to a conserved sequence GGGTCATGTGAGC (located at 568 to 556 in mouse TRAP promoter) containing an M-Box (underlined) (46). In agreement with its role in TRAP transcriptional activation, MITF was shown to be a target of RANKL action in osteoclasts (47). RANKL activates MITF by phosphorylating MITF at Ser307 via the p38 MAPK pathway (47). Furthermore, MITF regulates TRAP transcription in collaboration with PU.1 (48) and TFE-3 and TFE-C (49). TFE-3 and TFE-C are also characterized as bHLH-Zip proteins and are closely related to MITF (49). Our data not only identified USF1 and USF2 as additional members of the bHLH-Zip superfamily that play a critical role in controlling TRAP transcription but also raised questions on potential collaborations of USF1 and USF2 with the previously characterized bHLH-Zip transcription factors in regulating TRAP transcription in osteoclasts.
Although NF-B and AP-1 are two well-characterized transcription factors activated by RANKL in osteoclast differentiation, our present work reveals that RANKL regulates TRAP gene transcription by activating USF1 and USF2 (e.g. enhancing binding of USF1 and USF2 to the 12-bp RANKL-responsive sequence) in osteoclasts. How does RANKL activate USF proteins? One possibility is that RANKL increases the USF gene expression, and the enhanced USF binding directly results from the increase in amounts of USF proteins available. Alternatively, RANKL has no effect on the USF gene expression. Instead, RANKL activates USF proteins by phosphorylating them, and phosphorylated proteins have higher affinity for the binding site. The latter represents a more reasonable hypothesis because: 1) RANKL is able to activate the p38 MAPK pathway (50, 51, 52) and 2) the p38 MAPK pathway was shown to play a role in phosphorylating USF1 in mediating UV-induced Tyrosinase expression in a mouse melanocyte cell line (53). Nonetheless, the precise mechanism of the RANKL-mediated USF activation is currently under investigation. Elucidation of the signaling pathway involved in RANKL-mediated USF activation will provide more insights into the molecular mechanism by which RANKL regulates osteoclast differentiation.
Our initial transfection data showed that two distinct TRAP promoter regions are involved in regulation of TRAP transcription in response to RANKL (Fig. 2, A and B). Consistently, mutation of the USF-binding site in the region at 1239 to 1039 only partially blocked the RANKL-induced TRAP transcriptional activation (Fig. 8E), further supporting that the other region at 1858 to 1239 also contributes to the RANKL-induced TRAP transcriptional activation. To fully elucidate the molecular mechanism underlying RANKL-dependent TRAP gene activation, the cis-elements in the region at 1858 to 1239 need to be characterized. Furthermore, oligonucleotide IV binds one or more nuclear proteins (band C, lane 12, Fig. 2C) in response to RANKL. Given that mutant constructs TP(1199) and TP(1159) failed to confer RANKL responsiveness, the nuclear one or more proteins binding to oligonucleotide IV are not sufficient to activate TRAP promoter in response to RANKL. However, our data by no means exclude the possibility that the one or more nuclear proteins are necessary for RANKL-induced TRAP gene activation. Thus, investigation of the potential role of the nuclear protein(s) in RANKL-dependent TRAP transcription represents is warranted.
In conclusion, our data presented here demonstrate that USF1 and USF2 play an important role in RANKL-dependent TRAP transcription. This finding not only defines a role of USF proteins in TRAP expression in osteoclasts, but, more importantly, it also raises many important questions. First, whether and how USF proteins collaborate with other transcription factors in regulating TRAP transcription is unknown. Furthermore, the precise signaling pathway by which RANKL activates USF transcription factors remains to be elucidated. Future studies aimed at addressing these questions will provide more insights into molecular mechanisms governing osteoclast differentiation and function.
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FOOTNOTES |
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* This work was supported by National Institutes of Health (NIH) Grant AR-47830 (to X. F.), by the NIH Research Core Center/University of Alabama at Birmingham (UAB) Core Center for Musculoskeletal Disorders (Grant P30AR46031), by a National Osteoporosis Foundation grant (to X. F.), by a pilot grant from the Center for Aging at UAB (to X. F.), and by the American Cancer Society institutional research grants from the UAB Comprehensive Cancer Center (to X. F.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Both authors contributed equally to this work.
|| To whom correspondence should be addressed: Dept. of Pathology, University of Alabama, 1670 University Blvd., VH G046B, Birmingham, AL 35294. Tel.: 205-975-0990; Fax: 205-934-1775; E-mail: xfeng{at}path.uab.edu.
1 The abbreviations used are: M-CSF, monocyte/macrophage colony-stimulating factor; TRAP, tartrate-resistant acid phosphatase; USF, upstream stimulatory factor; BMMs, bone marrow macrophages; RANK, receptor activator of NF-B; RANKL, RANK ligand; TNF, tumor necrosis factor; EMSA, electrophoretic mobility shift Assay; bHLH-Zip, basic helix-loop-helix-leucine zipper domain; MITF, microphthalmia transcription factor; SOI, shortened oligonucleotide I; PBS, phosphate-buffered saline; DTT, dithiothreitol; MAPK, mitogen-activate protein kinase; AEBSF, 4-(2-aminoethyl)benzenesulfonyl fluoride.
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REFERENCES |
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