From the School of Biological Sciences, Division of Biochemistry, 2.205 Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom
Received for publication, March 7, 2003 , and in revised form, April 15, 2003.
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ABSTRACT |
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INTRODUCTION |
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Analyses of RNA processing in trypansomatids have largely focused on
trans-splicing. Trans-splicing has been shown to be
mechanistically similar to cis-splicing of introns in yeast and
higher eukaryotes, and the basic components of both processes are conserved
(for a review, see Ref. 4). In
contrast, little is known about the process of trypanosome mRNA 3'-end
formation, and no recognizable conserved motifs, such as the AAUAAA sequence
of higher eukaryotes, are present upstream of trypanosome polyadenylation
sites. Evidence also suggests that maturation of T. brucei
polycistronic pre-mRNAs involves a temporal and mechanistic relationship
between trans-splicing and 3'-end formation. It has been shown
that the pyrimidine-rich sequences immediately upstream of the -tubulin
trans-splice site are a major determinant for
-tubulin mRNA
3'-end formation (5).
Therefore, the sequence signals important for trans-splicing of the
downstream gene are believed to contribute to 3'-end formation of the
upstream gene in a polycistron. This is consistent with the possibility that
the polypyrimidine tract is recognized by both the trans-splicing and
polyadenylation machineries, either sequentially or simultaneously
(6). The nematode
Caenorhabditis elegans also undergoes trans-splicing during
mRNA maturation, and, as in trypanosomes, this organism is believed to couple
trans-splicing and polyadenylation to process precursor RNAs.
Although several studies have concentrated on the identification of the
components of the trans-splicing machinery in both T. brucei
and C. elegans (7,
8), there have been no studies
in either of these organisms concerning the cleavage and polyadenylation
machinery to date. Thus, the mechanistic links between the
trans-splicing machinery and 3'-end formation in polycistronic
RNA processing have only been the result of investigating polyadenylation as a
consequence of perturbations in the signals/machinery for
trans-splicing (5,
6,
9).
A major goal of investigating the mechanisms of pre-mRNA 3'-end processing is the identification and functional characterization of the different trans-acting factors involved in the reaction. In a previous study, we identified a family of proteins in T. brucei, possessing a CCCH zinc finger motif (10), characteristic of RNA-binding proteins. In this study, we have used genome data base mining to identify a protein containing five CCCH fingers and show it to be the cleavage and polyadenylation specificity factor 30-kDa subunit of T. brucei (tbCPSF30). Here, we specifically perturb tbCPSF30 by RNA interference and show that tbCPSF30 is essential for T. brucei survival in both the mammalian bloodstream and insect procyclic forms of the parasite. Interestingly, unprocessed tubulin transcripts accumulate when tbCPSF30 is depleted by RNA interference (RNAi),1 as has been seen previously when trans-splicing is disrupted. This implies that the integrity of the polyadenylation machinery as well as the trans-splicing machinery is required for polycistronic RNA processing. The significant sequence conservation of yeast, worm, fly, zebrafish, mammalian, and now T. brucei CPSF30 homologues, suggests that all of these are members of a family of proteins conserved from the earliest eukaryotes to vertebrates. Moreover, identification of other core component homologues of the yeast and mammalian CPSF in the T. brucei CPSF complex, by data base mining, suggests a basic conservation of the CPSF complex among eukaryotes with diverse mechanisms for regulating gene expression.
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EXPERIMENTAL PROCEDURES |
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Transfection of both bloodstream and procyclic form parasites was carried out as described in Ref. 10 using 10 µg of NotI (pHD451) or EcoRV (p2T7i) linearized DNA electroporated into 500 µl of cells at 4 x 107 cells ml1. Transfected cells were recovered overnight in 10 ml of HMI-9 at 37 °C, 5% CO2 (bloodstream forms), or SDM-79 medium at 27 °C (procyclic forms) before being diluted to 1 x 105 cells ml1 and subjected to drug selection in 24-well plates. Drug concentrations used for selection were hygromycin (2 µg ml1 for bloodstream forms, 2050 µg ml1 for procyclic forms), G418 (2.5 µg ml1 for bloodstream forms, 15 µg ml1 for procyclic forms), or phleomycin (0.52.5 µg ml1 for bloodstream forms, 5 µg ml1 for procyclic forms). Selected cells were cloned by limiting dilution under drug selection.
Transgenic Plasmid ConstructsThe trypanosome expression vector pHD451 (12) was modified to contain the TY1 epitope tag fused to the C terminus of genes cloned into the HindIII and BamHI sites. This vector was designated pHD451-TY1. The tbCPSF30 coding region was amplified by PCR using primers CPSF30.F and CPSF30.R (CPSF30.F is CC AAG CTT ATG TTT ACT GAC AAC GCT GCC C; CPSF30.R is GGG ATC CCT GCC TTC CCG TTG CAT CAC) and cloned into HindIII/BamHI-digested pHD451-TY1. This plasmid was then used to direct tbCPSF30 transgene expression in either bloodstream or procyclic form parasites that had been previously engineered to express the tetracycline repressor by stable transfection with the expression construct pHD449 (12).
The tbCPSF30 RNAi construct was made using the vector p2T7i (13), which allows for tetracycline-inducible expression of double-stranded RNA from a T7 promoter in T. brucei 427 SMB or 427 29-13 procyclic cells. The tbCPSF30 coding region was amplified using PCR primers CPSF30.F and CPSF30.R and cloned into the HindIII and BamHI sites of p2T7i. Further details of all constructs used in this paper are available upon request.
Total RNA Isolation, cDNA Synthesis, and Northern Analysis
Trypanosome RNA was prepared using a Qiagen RNA-easy kit (by the
manufacturer's method), and Northern blotting was performed as described
previously (14). Blots were
hybridized at 60 °C with riboprobes labeled with digoxygenin (Roche
Applied Science), and stringency washes were at 60 °C, 0.1x SSC.
Blot detection was by chemiluminescence using CDP-star as a reaction
substrate. The /
tubulin intergenic region riboprobe was
generated from a construct previously engineered in pBluescript II
(5).
Anti-peptide Antibody Production, Protein Isolation, and Western BlottingAn anti-peptide antibody to T. brucei CPSF30 was generated using two peptide antigens comprising the sequences LIERADDPSFNKNATC and QQWGGHRRGDATGRQ (see Fig. 1A). Both were coupled to keyhole limpet hemocyanin and used to immunize rabbits (Eurogentec, Belgium), the resulting serum being affinity-purified against each of the peptide antigens. Preimmune serum and immune serum were screened against whole cell trypanosome proteins. Preimmune serum did not show any reaction with T. brucei proteins.
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Proteins were prepared as described in Ref. 15 and resolved on 11% polyacrylamide gels. Blotted proteins were incubated with either the anti-TY1 epitope tag monoclonal antibody (BB2; diluted 1:20) or with the affinity-purified rabbit anti-tbCPSF30 antibody (diluted 1:20). Western blots were processed using horseradish peroxidase-conjugated secondary antibody and visualized by chemiluminescence (Amersham Biosciences).
Cell Growth, Microscopy, and Computational AnalysistbCPSF30
RNAi was induced by the addition of tetracycline (1 µg
ml1), and growth was monitored over a period of 2
weeks. At intervals, 2 x 106 cells were harvested,
spread onto microscope slides, and then air-dried for 10 min. The cells were
then fixed in methanol at 20 °C for at least 20 min. The cells were
rehydrated in PBS, and then slides were incubated with 4,6-diamidine-2,
phenylindole for 5 min. Finally, the cells were washed in PBS three times and
mounted in MOWIOL (Harlow Chemical Co., Kent, UK) containing phenylene diamine
(1 mg ml1). Slides were examined on a Zeiss
Axioscop 2 microscope, and images were captured using Scion Image version
1.62. Figures were processed using Adobe Photoshop 6.0. The tbCPSF30 locus was
mapped using the T. brucei data bases at the Sanger Institute
(available on the World Wide Web at
www.sanger.ac.uk/Projects/T_brucei/)
and at the Institute for Genomic Research (available on the World Wide Web at
www.tigr.org/tdb/mdb/tdbd/index.shtml).
These same databases were screened, using Blast analysis
(16), against both the yeast
and mammalian proteins known to be involved in 3'-end formation.
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RESULTS |
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The complete gene sequence was obtained by a combination of clone walking (within the data base) and PCR between the intervening sequence of contiguous clones. The resulting predicted sequence was then verified by sequencing the entire gene isolated from genomic DNA by PCR amplification. tBlastx analysis of the complete coding region demonstrated strong homology to the protein sequence for the cleavage and polyadenylation specificity factor 30-kDa subunit (CPSF30) from several organisms (34% identity and 65% similarity to yeast, 35% identity and 58% similarity to cow) (Fig. 1A). In addition to the conservation of the overall zinc finger motif structure (Fig. 1C), there were several further highly conserved regions of identity within the molecule and members of the CPSF30 family. On the basis of its strong homology to CPSF30 from several organisms, the gene was designated as the T. brucei cleavage and polyadenylation specificity factor 30-kDa subunit, tbCPSF30.
tbCPSF30 Is Expressed in Bloodstream and Procyclic Life Cycle
StagesIn Drosophila, the CPSF30 homologue (Clipper, or
CLP) is not ubiquitously expressed; it is absent during embryonic development
(19). Since tbCPSF30 and CLP
shared the greatest structural similarity (see
Fig. 1C), the
expression pattern of tbCPSF30 was assessed during the trypanosome
developmental cycle. Total RNA was prepared from bloodstream and procyclic
form parasites and hybridized with a riboprobe made to the coding region of
tbCPSF30. This analysis revealed that tbCPSF30 was expressed at both
these life cycle stages in similar abundance
(Fig. 2A). The message
was 1100 nucleotides, in agreement with the predicted size of the coding
region (831 bp) plus
200300 nucleotides of 5'- and
3'-untranslated regions. Southern analysis showed that tbCPSF30
was a single copy gene (data not shown).
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In the case of the Drosophila CLP, the protein and RNA expression
pattern do not coincide due to developmental regulation at the
post-transcriptional level
(20). To verify that the
expression pattern observed for tbCPSF30 at the RNA level also reflected
protein expression, an antibody was generated to two peptide antigens
(LIERADDPSFNKNATC and QQWGGHRRGDATGRQ) derived from the C terminus of
tbCPSF30, away from the conserved five CCCH zinc fingers and two zinc knuckle
motifs. Protein samples were prepared from both bloodstream and procyclic
forms and subjected to Western analysis with the specific tbCPSF30
anti-peptide antibody. The purified tbCPSF30 antibody detected two bands of
3234 kDa (Fig.
2B). This double band was always observed in several
different parental or wild type cell lines, although the relative proportion
of each did vary between samples. We believe that both bands are tbCPSF30 and
that they represent different forms of the protein (i.e. they may
represent a processed or post-transcriptionally modified form of the protein).
This has not been further examined; however, it is interesting to note that
in vitro purification of mammalian polyadenylation complexes also
indicated the presence of two forms of CPSF30 (28 and 30 kDa)
(21). Moreover, both bands
disappear upon tbCPSF30-specific transcript ablation (see below).
Fig. 2B confirms that
tbCPSF30 is expressed equally in both bloodstream and procyclic life cycle
stages, contrasting with the developmental regulation demonstrated by
Drosophila CLP.
Gene Silencing of tbCPSF30 Causes Cell DeathThe polyadenylation machinery comprises a complex of proteins of precise stoichiometry. We therefore assessed the functional consequences of ectopic overexpression and RNAi-mediated depletion of tbCPSF30. Initially, we used the trypanosome expression vector pHD451 (12) to generate bloodstream and procyclic form lines that overexpressed a TY-1 epitope-tagged copy of tbCPSF30. Transgenic tbCPSF30-TY could be clearly detected in both life cycle stages (Fig. 3A), being overexpressed 2-fold in bloodstream forms and 35-fold in the procyclic form with respect to the endogenous protein (data not shown). These cell lines were morphologically identical to the parental lines and grew at an identical rate (Fig. 3B). Thus, no adverse effects result from tbCPSF30 overexpression in either life cycle stage.
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Further functional analysis of the gene product was carried out using gene silencing by RNAi (22). The tbCPSF30 coding region was cloned into the vector p2T7i (13) and transfected into bloodstream SMB cells and procyclic 29-13 cells, which express the tetracycline repressor and T7 RNA polymerase, allowing tetracycline-inducible transcript ablation by RNAi. Transfected cells were induced with 1 µgml1 tetracycline, and cell growth was monitored for a period of 1214 days for both bloodstream and procyclic tbCPSF30 RNAi cell lines as well as for untransfected controls (Fig. 4, A and B). Cell morphology was also monitored during this period (Fig. 4, C and D). Upon induction with tetracycline, there was a striking growth phenotype in both bloodstream and procyclic tbCPSF30 RNAi cell lines but not in the control populations. Specifically, cells were able to proliferate for only 23 days, after which many dead cells were detectable in the induced bloodstream and procyclic populations. By 6 days postinduction, tbCPSF30 RNAi procyclic cell lines were dead or dying as shown in the growth profile (Fig. 4B) and the culture image (Fig. 4D). Similarly, the bloodstream population stopped proliferating by days 35, although some surviving cells were always detectable among a background of lysed cells (middle right panel in Fig. 4C). Interestingly, by day 68, the bloodstream cells had resumed growth, and at day 1214, their proliferation was indistinguishable from uninduced lines or the parental SMB line (Fig. 4, A and C). We predicted that the outgrowth of these cells represented selection for those unable to undergo tbCPSF30 RNAi.
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To assess the efficacy of RNAi in each population, we assayed the level of tbCPSF30 RNA at time points after induction with tetracycline. It was expected that the RNA levels for tbCPSF30 would show rapid ablation upon induction with tetracycline in bloodstream and procyclic forms. Surprisingly, however, Northern analysis showed that in both induced bloodstream and procyclic cell lines, an RNA of aberrant size was detected with the tbCPSF30 strand-specific riboprobe (Fig. 5). This RNA was slightly larger than the tbCPSF30 mRNA detected in uninduced or parental cell lines, and it was also increased in abundance when compared with normal tbCPSF30 mRNA levels (Fig. 5, arrows). This unexpected result was reproducible in several independently derived bloodstream and procyclic form cell lines. Significantly, the altered tbCPSF30 RNA was observed only in induced RNAi cell lines and disappeared upon outgrowth of the RNAi-resistant bloodstream forms when the correct sized tbCPSF30 transcript reappeared (Fig. 5, day 12 samples). We excluded the possibility that this novel transcript represented double-stranded RNA transcribed from the tbCPSF30 RNAi vector by hybridization with an antisense-specific riboprobe. This revealed that no detectable levels of tbCPSF30 double-stranded RNA were present in either the bloodstream or procyclic RNAi cell lines (data not shown). All of these facts strongly suggested that the observed tbCPSF30 RNA was tbCPSF30 RNAi-specific and did not represent a cryptic tbCPSF30 RNA derived from the tbCPSF30 RNAi 2T7i construct.
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To directly link the observed growth phenotype with tbCPSF30 expression, we
analyzed the protein levels for this molecule.
Fig. 6 shows protein samples
from induced and uninduced bloodstream tbCPSF30 RNAi cell lines probed with
the tbCPSF30 anti-peptide antibody. These samples show 8090%
reduction in the level of tbCPSF30 protein by day 34 upon induction
with tetracycline. This confirmed that gene silencing was operating in an
efficient and inducible manner. Consistent with the observation that the
bloodstream cells return to normal growth by day 10, the levels of tbCPSF30
protein returned to wild type levels by day 14
(Fig. 6). From this analysis,
it is clear that there is a strict correlation between the presence of
tbCPSF30 protein and the ability of T. brucei to survive in culture.
It can therefore be concluded that tbCPSF30 is essential for viability in both
the bloodstream and procyclic forms of T. brucei.
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tbCPSF30 Is Required for Polycistronic RNA ProcessingTo
investigate the consequences of tbCPSF30 depletion on trypanosome
polycistronic RNA processing, we examined the resolution of pre-mRNA in the
bloodstream and procyclic tbCPSF30 RNAi lines. In the case of the bloodstream
tbCPSF30 RNAi cells, RNA was prepared 2 days postinduction, and in the case of
the procyclic tbCPSF30 RNAi cells, samples were taken 3 days postinduction.
This corresponds to the period before cell death in each population. To assay
RNA processing, each RNA sample was hybridized with a riboprobe detecting the
/
-tubulin intergenic region. This probe detects the mature
-tubulin mRNA and unprocessed RNAs spanning the
/
-tubulin
dicistron. It has been reported
(5,
23) that perturbation of the
trans-splicing machinery results in an accumulation of dicistronic
/
-tubulin RNA due to disruption of processing of the tubulin gene
pre-mRNA. Our results in Fig.
7A clearly show the presence of an additional transcript
in both the bloodstream and procyclic tbCPSF30 RNAi-induced cell lines, which
is consistent with the size of a larger unprocessed, tubulin pre-mRNA
(dicistron). Significantly, this additional transcript is not seen in either
the wild type or uninduced cell lines for either of the life cycle stages of
the parasite.
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Further confirmation of the specificity of this result was provided by
analysis of the bloodstream samples that were tbCPSF30 RNAi-resistant
(i.e. those isolated 12 days after induction of tbCPSF30 RNAi). In
bloodstream tbCPSF30 RNAi samples taken 0, 2, and 12 days postinduction with
tetracycline, the increase in the unprocessed /
-tubulin
intergenic region was specific to the samples with depleted tbCPSF30, with the
outgrowing RNAi-resistant line behaving as wild type cells
(Fig. 7B). This
confirms that the perturbation of the processing of the tubulin polycistronic
RNA was a direct result of the gene silencing of tbCPSF30.
In order to eliminate the possibility that the appearance of polycistronic
RNA was simply the result of a general "dying cell" phenomenon, we
allowed the parental RNAi bloodstream line (SMB) to reach maximal growth
levels in culture (typically 5 x 106 cells/ml). At this
stage, cells are still intact and motile, although if left for 48 h without
passage, these cultures overgrow and rapidly die. Therefore, these cultures
were used as a population of cells undergoing several mechanisms (starvation,
cell cycle defects, cytotoxic effects) resulting in cell death.
Fig. 7C shows RNA from
the cultures hybridized with a probe for the /
-tubulin intergenic
region. It is clear that in these dying cells there was no specific increase
in unprocessed tubulin RNA. Rather, the accumulation of polycistronic RNA was
specific to the depletion of tbCPSF30, demonstrating that this molecule
contributes to the efficient processing of tubulin transcripts in T.
brucei.
Although trans-splicing is ubiquitous for all genes in T. brucei, it was recently discovered that at least one gene (poly(A)-polymerase (PAP)) also undergoes cis-splicing. This raised the question as to whether this RNA processing event might also be disrupted in tbCPSF30-depleted cells. Thus, cis-splicing was assayed in these cells using RT-PCR across the PAP intron. No change in cis-splicing efficiency for the PAP transcript was detectable (data not shown).
Components of the T. brucei RNA Processing Machinery Although there have been numerous studies of the components of both yeast and mammalian cleavage and polyadenylation complexes (for reviews, see Refs. 24 and 25), to date there have been no studies on the CPSF or associated factors in T. brucei. Since the T. brucei genome is organized into polycistrons, it is interesting to speculate that the core components of the polyadenylation and cleavage machinery might be different from other eukaryotes using monocistronic transcription. Consequently, by genome data base mining, we searched for components of the cleavage and polyadenylation machinery conserved in T. brucei (Table I). This revealed the presence of the previously cloned poly(A)-polymerase (26), a poly(A)-polymerase-binding protein homologue (PAPB1), and at least one other component of the cleavage and polyadenylation complex, CPSF73 (Table I). On the basis of sequence homology, other more putative component homologues of either yeast or mammalian proteins included CPSF100, CPSF160, CstF-50, CstF68, PFS2, MPE1, HRP1, and PAN2/3 (Table I). The PAP, CPSF30, CPSF73, CPSF100, and CPSF160 form core components of the cleavage and polyadenylation complex in yeast and mammals. Thus, our survey indicates that several components of the cleavage and polyadenylation machinery are already detectable in the incomplete T. brucei genome data base. This suggests that even in this evolutionarily ancient protozoan parasite, with a very usual mechanism of gene expression, the basic machinery for the processing of transcripts is similar to other lower eukaryotes as well as higher organisms.
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DISCUSSION |
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tbCPSF30 RNAi induction resulted in a specific increase in the level of a tbCPSF30 RNA, the first report showing an RNAi phenotype of this sort. Importantly, the appearance of this novel RNA precisely correlated with the ability of the cells to undergo tbCPSF30-specific RNAi (as assessed by cell death and protein depletion) and was lost upon outgrowth of RNAi-resistant bloodstream forms. The existence of this RNA can be rationalized in terms of recent understanding of the mechanism of RNA interference (27). This process is believed to be a cytoplasmic phenomenon effected by the action of siRNAs on mRNA, this being mediated by a complex of proteins conserved among species able to undergo RNAi. Recently, it has been suggested that RNAi operates at the level of the translational apparatus, whereby polyribosomal RNA is the target of degradation (28). In this scenario, the novel tbCPSF30 RNA that accumulates might represent nontranslated RNA protected from degradation by RNAi. This is directly supported by our observation that the aberrant RNA does not result in tbCPSF30 protein, which is specifically depleted at the same time that the novel RNA is maximally expressed. One interesting model would be that the novel tbCPSF30 RNA is compartmentalized within the cell, for example being contained within the nucleus, and accumulates as the cell attempts to compensate for tbCPSF30 protein depletion.
In eukaryotes, the transcription of specific genes and the formation of
mature mRNAs are processes that occur in the nucleus. Prior to nuclear export,
mRNAs require several posttranscriptional modifications, which are tightly
coupled. The precise mechanism that couples these post-transcriptional events
is still poorly understood. In Leishmania and Trypanosoma,
there is strong evidence that supports the model that polycistronic pre-mRNA
polyadenylation requires active trans-splicing or, minimally,
recognition of the 3' splice acceptor site
(5,
6,
29). Furthermore, studies in
C. elegans recently showed a physical interaction that couples the
trans-splicing and cleavagepolyadenylation machineries via the
cleavage stimulation factor 64-kDa subunit
(30). Interestingly, all of
these models have been proposed as a result of studies on
trans-splicing and the analysis of polyadenylation placed in that
context. In our study, depletion of tbCPSF30 has for the first time shown the
consequence of disrupting a component of the polyadenylation machinery. This
demonstrated a dramatic increase in the level of unprocessed
/
-tubulin RNA, revealing that tbCPSF30 is involved in the
maturation of polycistronic pre-mRNAs. Since the precursor RNAs require both
trans-splicing and polyadenylation for maturation, it is evident that
this accumulation can only occur if both processes are inhibited. This
suggests that the efficiency of trans-splicing is dependent upon the
integrity of the polyadenylation machinery as well as vice versa.
The cis-splicing machinery is similar in composition to that required in trans-splicing and, further, is mechanistically linked to 3'-end formation in higher eukaryotes (3134). Moreover, it has been shown that CPSF30 is essential for the cis-splicing of single-intron containing pre-mRNAs in vivo using the influenza NS1A protein (35). This led us to investigate the effect of tbCPSF30 depletion on the cis-splicing of T. brucei PAP, the only trypanosome gene known to contain an intron. However, we were unable to detect any difference in the ability to cis-splice the PAP gene in cells depleted for tbCPSF30. Although we cannot exclude a subtle effect on the efficiency of splicing, this suggests that CPSF30 is not essential for cis-splicing in T. brucei.
Although the control signals governing the overall process of 3'-end formation differ in higher and lower eukaryotes, several studies have shown functional similarity between the individual components of the machinery (3638). However, there are subtle but potentially important differences between the architecture of CPSF30 in different organisms. Of particular interest is the lack of the zinc knuckle CCHC motif in yeast compared with those organisms with one knuckle (zebrafish, mammals) and those with two (Drosophila, trypanosomes). It has been suggested that yeast utilize, by interaction, either the zinc knuckle in the uncharacterized PFS1 (36, 39, 40) or in the recently identified MPE1 (37) to compensate for the function of this part of the CPSF30 molecule from other organisms. It is interesting therefore that CPSF30 from trypanosomes seems to contain both these functional domains in one protein, despite the early divergence of these organisms from the eukaryotic lineage. This structural change between the molecules may also explain the failure of our attempts to functionally complement a yeast mutant with tbCPSF30 (data not shown).
Of the characterized components of the polyadenylation machinery in other organisms, only CPSF30 contains a known RNA binding domain, and this is thought to contribute to recognition of the polyadenylation signal sequence. Some debate has arisen over the endonuclease responsible for the first part of the reaction (41). The CPSF30 homologue in Drosophila (Clipper or CLP) specifically cleaves RNA hairpins, and this endonucleolytic activity resides in the region containing five copies of the CCCH zinc finger motif (19). The cleavage activity of the yeast protein also resides in a region containing the N-terminal CCCH zinc finger motif (39). Our data showing the requirement for tbCPSF30 in polycistronic RNA cleavage would support the theory that in vivo the CPSF30 is the endonuclease responsible for this initial reaction in 3'-end formation.
The identification of a trypanosome homologue of CPSF30 and the results of data mining the available trypanosome genome information suggest that the basic components of the T. brucei 3'-end RNA processing complex(es) are also similar to S. cerevisiae and mammalian systems. This observation is particularly significant, given that T. brucei diverged very early in eukaryotic evolution and exhibits a gene organization that necessitates that 3'-end formation and transcript termination be uncoupled. Clearly, organisms with very diverse mechanisms for gene expression conserve core elements of the machinery for cleavage and polyadenylation. Dissection of this complex will be fundamental to understanding the evolution of eukaryotic gene expression mechanisms and may provide new targets for the control of this important group of protozoan pathogens.
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FOOTNOTES |
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* This work was funded by the Wellcome Trust. The costs of publication of
this article were defrayed in part by the payment of page charges. This
article must therefore be hereby marked "advertisement"
in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
A Wellcome Trust University Fellow. To whom correspondence should be
addressed. Tel.: 44-161-275-5083; Fax: 44-161-275-5082; E-mail:
keith.matthews{at}man.ac.uk.
1 The abbreviations used are: RNAi, RNA interference; PAP,
poly(A)-polymerase.
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ACKNOWLEDGMENTS |
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REFERENCES |
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