From the Department of Biochemistry, University of Texas Health Science Center, San Antonio, Texas 78229-7760
Received for publication, January 7, 2003
![]() |
ABSTRACT |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Yeast NAD+-specific isocitrate
dehydrogenase (IDH) is an allosterically regulated octameric enzyme
composed of two types of homologous subunits designated IDH1 and IDH2.
Based on sequence comparisons and structural models, both subunits are
predicted to have adenine nucleotide binding sites. This was tested by
alanine replacement of residues in putative sites in each subunit.
Targets included adjacent aspartate/isoleucine residues implicated as important for determining cofactor specificity in related
dehydrogenases and a residue in each IDH subunit in a position occupied
by histidine in other cofactor binding sites. The primary kinetic
effects of D286A/I287A and of H281A replacements in IDH2 were found to
be a dramatic reduction in apparent affinity of the holoenzyme for NAD+ and a concomitant reduction in
Vmax. Ligand binding assays also showed that
the H281A mutant enzyme fails to bind NAD+ under conditions
that are saturating for the wild-type enzyme. In contrast, the primary
effect of corresponding D279A/D280A and of R274A replacements in IDH1
is a reduction in holoenzyme binding of AMP, with concomitant
alterations in kinetic and isocitrate binding properties normally
associated with activation by this allosteric effector. These results
suggest that the nucleotide cofactor binding site is primarily
contributed by the IDH2 subunit, whereas the homologous nucleotide
binding site in IDH1 has evolved for regulatory binding of AMP. These
results are consistent with previous studies demonstrating that the
catalytic isocitrate binding sites are comprised of residues primarily
contributed by IDH2, whereas sites for regulatory binding of isocitrate
are contributed by analogous residues of IDH1. In this study, we also
demonstrate that a prerequisite for holoenzyme binding of
NAD+ is binding of isocitrate/Mg2+ at the IDH2
catalytic site. This is comparable to the dependence of AMP
binding upon binding of isocitrate at the IDH1 regulatory site.
Mitochondrial NAD+-specific isocitrate dehydrogenase
(IDH)1 catalyzes a
rate-limiting step in the tricarboxylic acid cycle and is subject to
complex allosteric regulation. In particular, because of allosteric
activation of the mammalian enzyme by ADP (1) and of the yeast enzyme
by AMP, IDH is proposed to regulate metabolic flux in response to
energy needs of the cell (2). Saccharomyces cerevisiae IDH
is an octamer composed of four each of two homologous subunits, IDH1
and IDH2 (3). The mature polypeptides are similar in size (349 and 354 amino acid residues, respectively) and share 42% residue sequence
identity (4, 5). Both subunits are essential for holoenzyme structure
and function, although, as described below, catalytic function has been
primarily attributed to IDH2 whereas regulatory functions have been
assigned to IDH1 (6-8). Mammalian IDH contains three different types
of subunits that share significant homology with those of yeast IDH, an
Although crystallographic data are unavailable for yeast and mammalian
IDHs, these enzymes share substantial similarity in sequence with
several bacterial decarboxylating dehydrogenases for which
three-dimensional structures are available. As a family, these
dehydrogenases are unique in that they lack the classic Rossman fold
described for other enzymes that bind NAD(P)+ (11).
Particularly useful for analyses of yeast IDH are structures reported
for Escherichia coli isocitrate dehydrogenase (12-14) and
for Thermus thermophilus 3-isopropylmalate dehydrogenase
(15). The former is a homodimeric NADP+-specific enzyme
that functions in the bacterial tricarboxylic acid cycle, but that is
regulated by phosphorylation rather than by allostery (16). The latter
is a homodimeric NAD+-specific enzyme in the leucine
biosynthetic pathway. Consistent with catalysis of similar reactions,
these bacterial enzymes share some similarity in primary structure
(~25% residue identity) and substantial similarity in
three-dimensional structure. Differences between residues in catalytic
sites of the enzymes have been instructive for analyses of substrate
and cofactor specificity (17-19).
Both yeast IDH1 and IDH2 subunits share residue sequence identities of
~32% with E. coli isocitrate dehydrogenase. Based on sequence alignments and modeling, the catalytic
isocitrate/Mg2+ binding site of yeast IDH was predicted to
be primarily composed of residues from IDH2 (6), because IDH2 contains
identities for nine key residues in the catalytic site of the E. coli enzyme (12). IDH1, however, contains identities for only five
of these nine residues, and was proposed to bind but not catalytically alter isocitrate. These predictions have been confirmed by results summarized in Table I of site-directed
mutagenesis studies (6, 20-22). Among shared residues in isocitrate
binding sites, IDH1 and IDH2 each contain a serine residue analogous to
bacterial Ser-113. The latter is the site for phosphorylation of the
E. coli enzyme in vivo (16), a modification that
inactivates the enzyme by preventing binding of isocitrate (23).
Alanine replacement of the analogous Ser-98 in IDH2 was found to
profoundly reduce catalysis. Similar replacement in Ser-92 of IDH1 had
much less of an effect on catalytic capacity; however, it dramatically
reduced cooperativity and allosteric activation by AMP (6, 20).
Replacement of the serine residue in either yeast subunit eliminated
half of the holoenzyme isocitrate binding sites, and the combination of
these residue replacements in both subunits prevented isocitrate binding (Ref. 21 and Table I). Thus, both the IDH1 and IDH2 sites bind
isocitrate but for different kinetic functions. In other studies,
the four of nine residues that differ in each of the putative IDH1 and
IDH2 isocitrate binding sites were replaced by the corresponding
residues in the other subunit site (22). Mutant enzymes containing
these reciprocal residue replacements in IDH1 (A108R/F136Y/T241D/N245D)
and/or in IDH2 (R114A/Y142F/D248T/D252N) were found to retain the
wild-type number of isocitrate binding sites (Ref. 21 and Table I),
indicating that these replacements were permissive for binding at each
site. However, the mutant enzyme with residue replacements in IDH2
retained very little catalytic activity, and the mutant enzyme with
residue replacements in IDH1 exhibited no allosteric activation by
AMP nor any binding of AMP (20, 21). Thus, the unique residues in each
subunit isocitrate binding site are essential for different functions in catalysis (IDH2 site) or in allosteric regulation (IDH1 site).
INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
-subunit with catalytic functions similar to those of yeast IDH2,
and
- and
-subunits that are presumed to impart regulatory
properties (9-10).
Summary of effects of residue replacements in isocitrate sites of IDH
Based on these and other results, the homologous yeast IDH subunits appear to be an exceptional model for divergent evolution. The catalytic isocitrate/Mg2+ binding site in IDH2 has been highly conserved, and a similar isocitrate binding site in IDH1 has evolved for regulatory function. In the current study, we use mutagenesis to investigate putative nucleotide binding sites in each subunit. Our hypothesis is that residues important for cofactor NAD+ binding are primarily contributed by IDH2, and that the AMP binding site is comprised of analogous residues of IDH1. A corollary is that residues in the putative NAD+ binding site of IDH2 are likely to be evolutionarily conserved with residues in other cofactor binding sites, whereas analogous residues in the putative AMP binding site in IDH1 are likely to have diverged for binding of the structurally related allosteric activator.
Yeast IDH also displays complex interdependencies among various ligands
for binding to the enzyme (24). For example, the presence of isocitrate
is a prerequisite for AMP binding, and we have shown the specific
nature of this requirement is binding of isocitrate by the regulatory
IDH1 site (21). In this report, we also use mutant and wild-type
enzymes to investigate the dependence upon citrate or isocitrate to
obtain NAD+ binding and the dependence upon
Mg2+ for binding of other ligands of the enzyme.
![]() |
EXPERIMENTAL PROCEDURES |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Mutagenesis and Enzyme Expression-- For expression of wild-type and mutant forms of IDH in yeast, multicopy pRS426 plasmids (25) carrying both IDH1 and IDH2 genes were used. In these plasmids, each gene is preceded by authentic promoter sequences, and one of the 2-subunit genes contains codons for five histidine residues at the 3'-end of the coding region (7). We have previously shown that the histidine tag on the carboxyl terminus of either subunit facilitates affinity purification of holoenzyme and has no apparent effect on kinetic properties of the enzyme (7).
Site-directed mutagenesis was conducted using a
QuikChangeTM site-directed mutagenesis kit (Stratagene) to
replace codons for IDH1 Arg-274 and for IDH2 His-281 with codons for
alanine. Mutagenesis was conducted using a pBS plasmid
carrying the IDH1 gene and a pRS316 plasmid carrying the
IDH2 gene. Mutagenic oligonucleotides (with their
complementary oligonucleotides) were
5'-CTTCGAACCAGGTTCCGCCCATGTTGGTTTAGATATTAAAG for the R274A
replacement in IDH1 and
5'-CGATCTTTGAAGCTGTCGCTGGCTCTGCCCCTGATATTG for the H281A
replacement in IDH2. DNA sequence analysis was conducted to ascertain
the presence of only the desired mutations. DNA fragments containing
the altered IDH1 and IDH2 genes were subcloned
into pRS426 plasmids described above for expression with the wild-type gene for the other subunit.
Mutagenesis of IDH1 and IDH2 genes for alanine replacement of adjacent Asp-279 and Ile-280 residues in IDH1 and of adjacent Asp-286 and Ile-287 residues in IDH2 was previously described (7). For current studies, IDH1D279A/I280A/IDH2 and IDH1/IDH2D286A/I287A gene pairs were transferred on 6.4-kbp SstII/HindIII DNA fragments from centromere-based plasmids into pRS426. Some experiments also used pRS426 plasmids constructed as previously described (6, 20, 22) for expression of the following mutant forms of IDH: IDH1S92A/IDH2, IDH1/IDH2S98A, IDH1A108R,F136Y,T241D,N245D/IDH2, and IDH1/ IDH2R114A,Y142F,D248T,D252N.
Plasmids were transformed into yeast strain IDH12L (MAT
ade2-1 can1-100 his3-11,15 leu2-3,112 trp101 ura301
idh1::LEU2
idh2::HIS3; 26) containing
deletion/disruption mutations in chromosomal IDH1 and
IDH2 loci. Transformants were selected and maintained on
agar plates containing YNB medium (0.17% yeast nitrogen base, 0.5%
ammonium sulfate, pH 6.5) with 2% glucose and nutrients as needed for
selection and growth.
To assess cellular levels of expression, yeast transformants were grown in YP medium (1% yeast extract, 2% Bacto-peptone) containing 2% ethanol as the carbon source. Protein extracts were prepared by glass bead lysis of harvested cell pellets as previously described (27). Samples with equivalent protein concentrations, determined by Bradford assays using bovine serum albumin as the standard (28), were loaded onto 10% polyacrylamide/sodium dodecyl sulfate gels. Following electrophoresis, the proteins were transferred to a polyvinylidine difluoride membrane for immunoblot analysis using an anti-yeast IDH antiserum (3). The enhanced chemiluminescence method (ECL, Amersham Biosciences) was used for detection.
Enzyme Purification--
For purification of wild-type and
mutant forms of IDH, yeast transformants were grown as previously
described in YP ethanol medium (21). Cell pellets were harvested from
1-2 liters of culture to obtain enzyme for kinetic assays or from 6-8
liters of culture to obtain enzyme for ligand binding assays. Cell
pellets were stored at 70 °C prior to breaking. Affinity
purification was conducted as previously described (7) using
Ni2+-NTA resin (Qiagen). Concentrations of purified enzymes
were measured using absorbance values at
A280 nm and a molar extinction coefficient for
the octameric holoenzyme of 168820 M
1 cm
1 (29). Purity of affinity-purified enzymes was assessed
by electrophoresis as described above followed by staining with
Coomassie Blue. Yields of purified enzymes (0.4-1.0 mg/g of cell
pellet) were comparable for the wild-type and for mutant enzymes with
the exception of the IDH1/IDH2D286A,I287A mutant enzyme.
Yields of the latter enzyme were ~100-fold lower despite apparently
equivalent cellular levels of expression as described in the text.
Modifications to the purification procedure, including addition of a
variety of protease inhibitors during cell breakage and conducting
column chromatography at 4 °C, failed to improve yields of the
IDH1/IDH2D286A,I287A enzyme.
Kinetic and Ligand Binding Assays-- Standard assays for measuring isocitrate dehydrogenase activity contained 40 mM Tris-HCl (pH 7.4), 4 mM MgCl2, 0.5 mM NAD+, and 1.0 mM D-isocitrate (calculated as 50% of the total concentration of DL-isocitrate). These concentrations of NAD+ and isocitrate produce velocities 80-90% of Vmax measured for IDH and permit detection of allosteric effects. For isocitrate kinetic saturation curves, concentrations of D-isocitrate were varied from 0 to 1.5 mM for the wild-type enzyme and to as high as 10 mM for some mutant enzymes. Assays were conducted in the absence or presence of 100 µM AMP. For NAD+-saturation curves, isocitrate concentrations were set at five times the measured S0.5 value for each enzyme, and NAD+ concentrations were varied from 0 to 2.0 mM for the wild-type enzyme and to as high as 10 mM for some mutant enzymes. Assays were initiated by addition of enzyme (~1 µg of wild-type enzyme and as much as 12 µg of some mutant enzymes per 1.0-ml assay). A unit of activity is defined as production of 1 µmol of NADH/min at 24 °C. Values for kinetic parameters represent the average of two independent experimental determinations and were obtained by sigmoidal Hill plots of initial velocity data.
Ligand binding analyses were performed using an ultrafiltration method
as previously described (21). Assays contained ~1.0 mg of
affinity-purified enzyme/ml of binding buffer (40 mM
Tris-HCl, pH 7.4, and 4 mM MgCl2). For
isocitrate ligand binding assays, D-isocitrate
concentrations were varied from 0 to 1.5 mM, and assays
were conducted in the presence or absence of 100 µM AMP. Isocitrate concentrations in ultrafiltrates were measured enzymatically as previously described (21). For NAD+ binding assays, the
binding buffer contained 2.0 mM citrate, and
NAD+ concentrations were varied from 0 to 4.0 mM. For AMP binding assays, the binding buffer contained
1.0 mM D-isocitrate, and AMP concentrations
were varied from 0 to 0.4 mM. NAD+ and AMP
concentrations in ultrafiltrates were measured spectrophotometrically. In some experiments described in the text, MgCl2 was
omitted in ligand binding buffers. Binding is expressed as mol of bound
ligand/mol of IDH holoenzyme (Mr, 303,024).
![]() |
RESULTS |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Construction of Mutant Enzymes--
To identify and alter residues
of yeast IDH involved in binding NAD+ and AMP, we
previously used a strategy based on a comparison of residues involved
in NADP+ binding by E. coli isocitrate
dehydrogenase (12, 13) and in NAD+ binding by T. thermophilus 3-isopropylmalate dehydrogenase (15, 17). Many
residues involved in cofactor binding are conserved in the two
bacterial enzymes, but among differences are those that interact with
the ribose-2'-phosphate in the NADP+-specific enzyme. The
latter include adjacent Lys-344 and Tyr-345 residues in E. coli isocitrate dehydrogenase (Ref. 13 and Fig. 1). This lysine/tyrosine pair is
conserved in other NADP+-specific enzymes (17, 18), and Lee
and Colman (30) have recently shown that analogous Arg-314 and Tyr-316
residues of the homodimeric mammalian mitochondrial
NADP+-specific isocitrate dehydrogenase function in binding
of the cofactor. In contrast to the NADP+-specific enzymes,
NAD+-specific dehydrogenases including the T. thermophilus enzyme and both subunits of IDH contain adjacent
aspartate and isoleucine residues in analogous residue positions (Fig.
1). The importance of these adjacent residues in determining cofactor
specificity was confirmed by Chen et al. (17), who reported
that altering the cofactor specificity of E. coli isocitrate
dehydrogenase requires seven residue replacements including K344D and
Y345I. To test the roles of adjacent Asp-286 and Ile-287 residues in
the yeast IDH2 subunit and of adjacent Asp-279 and Ile-280 residues in
IDH1, we previously replaced each pair with adjacent alanine residues and reported preliminary kinetic characterization (7). The current
study extends these results and includes direct ligand binding
analyses.
|
Secondary targets for nucleotide binding sites in the yeast IDH subunits were chosen based on a report by Huang and Colman (31) that replacement of His-309 in mammalian mitochondrial NADP+-specific isocitrate dehydrogenase dramatically reduces activity and cofactor binding. This residue is conserved in the NAD+- and NADP+-specific bacterial enzymes (Fig. 1). In the E. coli enzyme crystal structure, the analogous His-339 is located near the adenine ring (13, 18). In the yeast IDH subunits, the analogous residue position is occupied by His-281 in IDH2 and by Arg-274 in IDH1 (Fig. 1). We therefore constructed mutant enzymes containing alanine residues for each of these residues to test effects on kinetics and nucleotide binding properties of the enzyme.
Mutagenesis was conducted as described under "Experimental
Procedures." Wild-type and mutant enzymes were expressed using multicopy plasmids carrying both IDH1 and IDH2
genes in a yeast strain containing disruptions of the endogenous
IDH1 and IDH2 loci. Expressed enzymes contain
pentahistidine tags on the carboxyl terminus of one of the two subunits
(7). As illustrated in Fig.
2A, cellular protein extracts
from yeast transformants contain approximately equivalent
immunochemical levels of wild-type enzymes (lanes a and
1) and of mutant enzymes (lanes 2-5). With
enzymes carrying the histidine tag on IDH1, IDH1, and IDH2 subunits are electrophoretically distinct (lanes 1-3 and 5),
but with enzymes carrying the histidine tag on IDH2, the subunits
comigrate (lanes a and 4). The enzymes were
purified from cellular extracts using Ni2+-NTA column
chromatography. As illustrated in Fig. 2B, the wild-type enzyme used in this study (IDH1His/IDH2, lane 1)
and mutant enzymes including IDH1R274A/IDH2 (lane
2), IDH1/IDH2H281A (lane 3), and
IDH1D279A,I280A/IDH2 (lane 4) were similarly
purified. However, despite apparently normal cellular levels of
expression of the IDH1/IDH2D286A,I287A enzyme (Fig.
2A, lane 5), exceptionally low relative yields of this enzyme (Fig. 2B, lane 5) were obtained
during purification procedures conducted under a variety of conditions.
This suggests that the structural integrity of this mutant enzyme is
highly compromised, producing a susceptibility to proteolysis or
disassembly during cell breakage and purification. Our inability
to purify sufficient amounts of the IDH1/IDH2D286A,I287A
enzyme precluded ligand binding analyses.
|
Kinetic Parameters and Isocitrate Binding Properties--
To
obtain saturation curves for isocitrate with affinity-purified
wild-type and mutant enzymes, kinetic assays, and ligand binding assays
were conducted in the absence or in the presence of 100 µM AMP. Examples of these curves are shown in Fig.
3. Saturation velocity curves for
NAD+ were also obtained with kinetic assays conducted in
the presence of AMP. Kinetic parameters for isocitrate and
NAD+ are presented in Table
II.
|
|
Kinetic data from isocitrate saturation curves for the IDH1/IDH2H281A and IDH1/IDH2D286A,I287A enzymes (Fig. 3A and Table II) show that the major effect of residue substitutions in the putative NAD+ binding site of the enzyme is a decrease in apparent Vmax values. This value is ~9-fold lower for the IDH1/IDH2H281A enzyme and ~300-fold lower for the IDH1/IDH2D286A,I287A enzyme relative to that of the wild-type enzyme. Despite a significant difference in the degree of catalytic dysfunction, both mutant enzymes retain essentially wild-type characteristics of cooperativity with respect to isocitrate (Hill coefficients of 3.5-4.1) and of allosteric activation by AMP. The latter property is evident by an ~5-fold decrease in the S0.5 value for isocitrate when measured in the presence of AMP. Kinetic data from NAD+-saturation curves (Table II) further suggest that the major effect of these residue substitutions in IDH2 is on affinity for NAD+. The S0.5 values for NAD+ are increased ~30-fold for the IDH1/IDH2H281A enzyme and ~40-fold for the IDH1/IDH2D286A,I287A enzyme. However, with high concentrations of cofactor, the apparent Vmax value for the IDH1/IDH2H281A enzyme is essentially equivalent to that of the wild-type enzyme, whereas the velocity of the IDH1/IDH2D286A,I287A enzyme is unaffected. These data suggest that both mutant enzymes with residue substitutions in IDH2 have reduced affinity for NAD+, but that the D286A/I287A substitutions are much more detrimental than the H281A substitution to cofactor binding and to catalytic function. These kinetic data further suggest that both IDH2 mutant enzymes retain wild-type characteristics with respect to binding of isocitrate and of AMP.
The isocitrate binding properties of the IDH1/IDH2H281A enzyme were analyzed and found to be quite similar to those of the wild-type enzyme (Fig. 3B and Table III). Both mutant and wild-type enzymes have four isocitrate binding sites, and an equivalent AMP effect (an ~3.5-fold decrease in the KD value for isocitrate) is observed. As described above, we were unable to obtain ligand binding data for the IDH1/IDH2D286A, I287A enzyme.
|
More moderate effects on Vmax in isocitrate saturation curves are produced by the R274A and D279A/I280A residue substitutions in the putative AMP binding sites of IDH1 (Fig. 3A and Table II). The IDH1R274A/IDH2 enzyme exhibits an ~8-fold decrease in velocity in the absence of AMP, comparable to that produced with the corresponding H281A substitution in IDH2. However, the decrease in velocity of the IDH1R274A/IDH2 enzyme is less (~5-fold) when measured in the presence of AMP and, in addition, an apparent Vmax value only ~2-fold lower than wild-type is obtained in NAD+ kinetic saturation curves (Table II). Thus, high concentrations of NAD+ have a substantial effect on velocity of the IDH1R274A/IDH2 enzyme, which also exhibits an ~4-fold increase in the S0.5 value for NAD+. In comparison, the apparent Vmax values for the IDH1D279A,I280A/IDH2 mutant enzyme measured under several conditions are consistently ~2-fold less than wild-type values. This relatively mild effect of D279A/I280A substitutions in IDH1 on catalysis contrasts sharply with the dramatic decrease in velocity obtained with corresponding D286A/I287A substitutions in IDH2.
Both types of residue substitutions in IDH1 produce defects in AMP activation measured in kinetic and ligand binding saturation curves with isocitrate. For the IDH1R274A/IDH2 enzyme, the apparent affinity for isocitrate in kinetic assays is actually less in the presence than in the absence of AMP (Fig. 3A and Table II). For the IDH1D279A,I280A/IDH2, AMP produces only a 1.5-fold decrease in the kinetic S0.5 value for isocitrate. Ligand binding assays conducted with both mutant enzymes (Fig. 3B and Table III) indicate the loss of any effect of AMP on isocitrate binding. Thus, a primary defect in both IDH1 mutant enzymes is loss of AMP activation. However, there are several significant differences between these mutant enzymes. The IDH1R274A/IDH2 enzyme exhibits a substantial loss of cooperativity with respect to isocitrate binding and kinetics (Hill coefficients of 1-2). This is not observed for the IDH1D279A,I280A/IDH2 enzyme. Also, as described above, the apparent Vmax of the IDH1R274A/IDH2 enzyme, but not that of the IDH1D279A,I280A/IDH2 enzyme, is affected by the presence of AMP or of increasing concentrations of NAD+. Thus, with respect to isocitrate kinetic and ligand binding properties, the primary defect associated with the D279A/I280A substitutions appears to be loss of AMP activation, whereas defects associated with the R274A substitution are more pleiotropic but include a loss of AMP activation.
Nucleotide Binding Analyses--
Ligand binding assays for
NAD+ and AMP were performed with purified wild-type and
mutant enzymes as described under "Experimental Procedures."
Saturation curves are illustrated in Fig.
4 and binding parameters are summarized
in Table III. Approximately two binding sites for
NAD+/holoenzyme are measured in saturation plots (Fig.
4A) for the wild-type enzyme and for mutant enzymes with
R274A and D279A/I280A substitutions in IDH1. KD
values for NAD+ binding (Table III) are similar for
wild-type and IDH1D279A/I280A/IDH2 enzymes. The
KD value for NAD+ binding is elevated
~3-fold for the IDH1R274A/IDH2 mutant enzyme, consistent
with a similar decrease in affinity for cofactor implied by kinetic
data. In contrast, under these conditions, no NAD+ binding
is measurable for the IDH1/IDH2H281A mutant enzyme (Fig.
4A). Kinetic data suggest that the
IDH1/IDH2H281A mutant enzyme should be saturable with very
high cofactor concentrations, but these exceed concentrations
measurable with our ligand binding assays.
|
With respect to AMP binding (Fig. 4B and Table III), we find that the wild-type enzyme and IDH1/IDH2H281A mutant enzyme exhibit essentially identical characteristics, with two measurable binding sites/holoenzyme and similar KD values. In contrast, under these conditions, the IDH1R274A/IDH2 and IDH1 D279A/I280A/IDH2 mutant enzymes exhibit no measurable binding of the allosteric activator.
Overall, these ligand binding data are consistent with kinetic results. They suggest that the major effect of residue replacements in IDH2 (H281A and, based on kinetic data, D286A/I287A) is a reduction in affinity for NAD+ and, consequently, a decrease in catalytic capacity. In contrast, the major effect of residue replacements in IDH1 (R274A and D279A/I280A) is a reduction in affinity for AMP, with consequent effects on allosteric activation. Thus, residues in similar positions in the two subunits support the binding of different nucleotides.
Interrelationships in Nucleotide Binding--
As originally
described by Kuehn et al. (24), yeast IDH displays a complex
interdependence among various ligands for binding. For example, AMP
binding requires the presence of isocitrate. As illustrated in Fig.
5A, we previously found that
isocitrate concentrations of 200 µM are needed to
obtain saturable binding of AMP (21). We further utilized mutant forms
of IDH to examine this requirement. We found that, under conditions
producing saturable binding of AMP by the wild-type enzyme (Fig.
5B,
), an IDH1S92A/IDH2 mutant enzyme, which
is deficient in binding of isocitrate at the regulatory site (Ref. 21
and Table I), fails to bind AMP (Fig. 5B,
).
Furthermore, an IDH1A108R,F136Y,T241D,N245D/IDH2 mutant
enzyme, which contains replacements for unique residues in the IDH1
isocitrate binding site with corresponding residues contained in the
IDH2 isocitrate binding site and which retains the wild-type number of
four isocitrate binding sites/holoenzyme (Ref. 21 and Table I), also
fails to bind AMP (Fig. 5B,
). These results suggested
that prerequisites for binding of allosteric activator include both
binding of isocitrate at the regulatory site in IDH1 and, in addition,
subsequent changes in the enzyme normally elicited by the binding of
isocitrate by authentic residues (i.e. Ala-108, Phe-136,
Thr-241, and Asn-245) in the regulatory site.
|
In the current study, we similarly used mutant enzymes to investigate
the reported (iso)citrate requirement for NAD+ binding by
IDH (24). Citrate is used as an analogue for isocitrate in these
binding assays to preclude catalysis. As illustrated in Fig.
5C, no NAD+ is bound in the absence of citrate,
and concentrations of citrate 500 µM are necessary to
obtain saturable binding of NAD+. Since this requirement
presumably involves (iso)citrate binding at the catalytic IDH2 site, we
examined NAD+ binding using mutant enzymes with residue
replacements in this site. As illustrated in Fig. 5D, an
IDH1/IDH2S98A mutant enzyme (
), which is deficient in
isocitrate binding at the IDH2 site (Ref. 21 and Table I), fails to
bind NAD+ under conditions producing saturable binding by
the wild-type enzyme (
). This is not the case for a
IDH1/IDH2R114A,Y142F,D248T,D252N mutant enzyme, which
contains reciprocal replacements for unique residues in the IDH2
isocitrate binding site with corresponding residues from the IDH1
isocitrate binding site. This mutant enzyme, although essentially
inactive (20), retains the wild-type number of four isocitrate
binding sites (Ref. 21 and Table I). As shown in Fig. 5D
(
), the IDH1/IDH2R114A,Y142F,D248T,D252N mutant enzyme
also retains essentially wild-type characteristics of NAD+
binding. Thus, in contrast to the prerequisites described above for AMP
binding, (iso)citrate binding at the IDH2 site appears to be sufficient
for cofactor binding, i.e. the authentic residues (i.e. Arg-114, Tyr-142, Asp-248, and Asp-252) in the IDH2
site are not essential for this function.
We have further analyzed the reported dependence upon the presence of
Mg2+ for binding of other ligands by wild-type IDH (24).
Catalysis requires a divalent cation (32), and a complex of
isocitrate/Mg2+ is bound to the catalytic site of the
E. coli enzyme (13), suggesting that
isocitrate/Mg2+ is the substrate that binds to the IDH2
catalytic site. However, residue differences suggest that the IDH1
isocitrate binding site binds isocitrate but not Mg2+
(21). Also, Kuehn et al. (24) found that yeast IDH has twice as many isocitrate as Mg2+ binding sites. We therefore
compared isocitrate binding (±100 µM AMP) by the
wild-type enzyme in the presence and
absence of Mg2+. As illustrated in Fig. 6A and
as summarized in Table IV, the total
number of isocitrate binding sites/holoenzyme is reduced from four to
two in the absence of Mg2+. However, the overall affinity
of the enzyme for isocitrate and the AMP effect on the
KD value for isocitrate are largely unaffected by
the absence of Mg2+. These results suggest that the
divalent cation is required for binding of isocitrate by half of the
isocitrate binding sites (presumably the catalytic IDH2 sites) but not
for binding to other sites (presumably the regulatory sites provided by
IDH1).
|
|
Since (iso)citrate binding subsequently affects binding of other
ligands, we also tested the requirements for Mg2+ for
binding of nucleotides by IDH. As shown in Fig. 6B, IDH
exhibits no binding of NAD+ in the absence of
Mg2+. This is presumably because of the dependence of
cofactor binding on the binding of (iso)citrate at the catalytic site
which, in turn, requires the presence of divalent cation. In contrast,
binding of AMP by IDH is largely unaffected by the absence of
Mg2+ (Fig. 6C and Table IV). This observation is
consistent with the assumption that isocitrate binding at regulatory
sites in IDH1 is independent of divalent cation, and that binding of
isocitrate alone at this site supports subsequent binding of the
allosteric activator.
![]() |
DISCUSSION |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Kinetic and ligand binding analyses described in this report suggest that yeast IDH has distinct but homologous nucleotide binding sites for NAD+ and for AMP. The cofactor binding site contains residues of the IDH2 subunit that are homologous with those in cofactor binding sites of other NAD+-specific decarboxylating dehydrogenases (Fig. 1). Adjacent Asp-286 and Ile-287 residues of IDH2 apparently correspond with adjacent Asp-279 and Ile-280 residues of T. thermophilus 3-isopropylmalate dehydrogenase, residues implicated as important for cofactor specificity (17, 18). In NADP+-specific isocitrate dehydrogenases, equivalent positions are occupied by adjacent or nearby lysine (or arginine) and tyrosine residues. Thus, replacement of IDH2 Asp-286 and Ile-287 with alanine residues produces a dramatic reduction in apparent affinity for NAD+ and a concomitant reduction in the catalytic capacity of IDH. Similar effects on affinity for NADP+ and on velocity were reported for mutant enzymes containing replacements for Arg-314 and Tyr-316 residues of mammalian mitochondrial NADP+-specific isocitrate dehydrogenase (30). In addition, His-281 of IDH2 is important for cofactor binding, since the most dramatic effect of alanine replacement of this residue is a reduction in affinity for NAD+. His-281 thus appears to be the functional homologue of a specific histidine residue in the cofactor binding sites of the T. thermophilus enzyme (His-274, Ref. 15), of the mammalian enzyme mentioned above (His-309, Ref. 31), and of E. coli isocitrate dehydrogenase (His-339, Refs. 13 and 18). Unlike the aspartate/isoleucine or lysine/tyrosine pairs described above, this histidine residue apparently functions in binding NAD+ or NADP+ but is not a determinant of cofactor specificity.
The yeast IDH1 subunit also contains an aspartate/isoleucine pair at residue positions 279 and 280, but contains an arginine in residue position 274 that aligns with the histidine in position 281 of IDH2. We have shown that the primary effect associated with alanine replacements for both types of residues in IDH1 is a significant reduction in holoenzyme affinity for AMP. The consequence is a defect in allosteric activation, i.e. for these mutant enzymes, isocitrate binding is unaffected by the presence of AMP. Despite similar effects on holoenzyme affinity for AMP, the D279A/I280A and R274A replacements in IDH1 have different kinetic effects. The latter replacement has a greater effect on apparent Vmax values and significantly reduces cooperativity with respect to isocitrate. These results suggest that, in addition to participating in binding of AMP, IDH1 Arg-274 may function in communication between regulatory and catalytic sites.
Overall, results obtained in this study suggest that related but unique sites have evolved in the homologous subunits to facilitate binding of different nucleotide ligands of IDH. The differential functions of IDH1 and IDH2 subunits in nucleotide binding are consistent with previous results showing that both subunits also contribute isocitrate binding sites (6-8, 20, 21), but that the site comprised primarily of residues from IDH2 is catalytic whereas the site comprised primarily of residues from IDH1 supports regulatory properties of the holoenzyme. Thus, the catalytic isocitrate/Mg2+- and NAD+ binding sites are contributed by IDH2, whereas the regulatory isocitrate- and AMP binding sites are contributed by IDH1. As might be expected, the IDH2 catalytic site(s) exhibits a more significant conservation of residues in catalytic sites of related enzymes (20). In the case of IDH1, some residue conservation is observed for binding of structurally related ligands, but key residue differences appear to be important elements in the evolution of regulatory properties of this complex allosteric enzyme.
A key to the communication between catalytic and regulatory sites in IDH is that, while each ligand binding site is primarily comprised of residues from one type of subunit, a few residues are apparently contributed by the other type of subunit. For example, results of previous mutagenesis studies (8) are consistent with the conclusion that of nine residues in the IDH2 catalytic isocitrate/Mg2+ binding site, two are contributed by IDH1 and, reciprocally, the analogous two residues of nine in the IDH1 regulatory isocitrate binding site are contributed by IDH2. These and other results from yeast two-hybrid studies (8) support a model for a heterodimer of IDH1 and IDH2 subunits as the basic structural/functional unit of the holoenzyme. Consistent with reciprocal subunit contributions to isocitrate binding sites, we have also reported kinetic analyses of other mutant enzymes that indicate similar potential intersubunit contributions to nucleotide binding sites (7). These studies will be expanded by replacement of other residues with similar putative intersubunit functions and by direct ligand binding analyses.
In current and previous studies (21), we have also evaluated some of the complex interrelationships among various ligands for binding by yeast IDH. Cumulative results suggest the following conclusions: (A) Despite residue differences in the isocitrate binding sites, the catalytic site in IDH2 and the regulatory site in IDH1 bind isocitrate with similar affinity. Binding at catalytic and regulatory sites is independent, since isocitrate binding at either site is not affected by loss of isocitrate binding at the other site (21). (B) Nucleotide binding sites are also independent of each other, because residue replacements examined in this study primarily affect either NAD+ or AMP binding (Fig. 4). (C) Binding of isocitrate by the catalytic IDH2 site, but not by the regulatory IDH1 site, requires Mg2+. Thus, the substrate bound by the enzyme is a complex of isocitrate/Mg2+. Furthermore, that isocitrate binding by the IDH1 site is independent of Mg2+ is additional evidence for evolutionary divergence of this site for regulatory rather than catalytic function. (D) A prerequisite for binding of NAD+ is binding of the (iso)citrate/Mg2+ complex by the catalytic IDH2 site. No binding of cofactor is observed in the absence of either (iso)citrate or Mg2+. (E) A prerequisite for binding of AMP is binding of isocitrate by the IDH1 regulatory binding site. An additional prerequisite for binding of AMP is some subsequent change(s) in the holoenzyme elicited by isocitrate binding at the IDH1 site, since replacement of the four non-identical of nine residues in the IDH1 isocitrate binding site with corresponding residues from the IDH2 site is permissive for isocitrate binding but not for AMP binding (21).
Collectively, these results suggest that only isocitrate binding by the
regulatory IDH1 site occurs in the absence of other ligands of the
enzyme. The complex prerequisites for binding of other ligands may
reflect mechanisms for tight control of IDH in vivo,
e.g. to ensure that binding and sequestering of a common tricarboxylic acid cycle cofactor occurs only in the presence of
sufficient substrate (isocitrate/Mg2+), and to ensure that
allosteric activation by AMP occurs only when concentrations of
isocitrate are sufficiently elevated.
![]() |
ACKNOWLEDGEMENTS |
---|
We thank Dr. Karyl I. Minard for technical advice, and Drs. Minard and Mark T. McCammon for critical reading of this manuscript.
![]() |
FOOTNOTES |
---|
* This work was supported by National Institutes of Health Grant GM51265.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 210-567-3782;
Fax: 210-567-6595; E-mail: henn@uthscsa.edu.
Published, JBC Papers in Press, January 31, 2003, DOI 10.1074/jbc.M300154200
![]() |
ABBREVIATIONS |
---|
The abbreviations used are: IDH, isocitrate dehydrogenase; NTA, nitrilotriacetic acid.
![]() |
REFERENCES |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
1. | Chen, R. F., and Plaut, G. W. E. (1963) Biochemistry 2, 1023-1032 |
2. |
Hathaway, J. A.,
and Atkinson, D. E.
(1963)
J. Biol. Chem.
238,
2875-2881 |
3. | Keys, D. A., and McAlister-Henn, L. (1990) J. Bacteriol. 172, 4280-4287[Medline] [Order article via Infotrieve] |
4. |
Cupp, J. R.,
and McAlister-Henn, L.
(1991)
J. Biol. Chem.
266,
22199-22205 |
5. |
Cupp, J. R.,
and McAlister-Henn, L.
(1992)
J. Biol. Chem.
267,
16417-16423 |
6. | Cupp, J. R., and McAlister-Henn, L. (1993) Biochemistry 32, 9323-9328[Medline] [Order article via Infotrieve] |
7. |
Zhao, W.-N.,
and McAlister-Henn, L.
(1997)
J. Biol. Chem.
272,
21811-21877 |
8. |
Panisko, E. A.,
and McAlister-Henn, L.
(2001)
J. Biol. Chem.
276,
1204-1210 |
9. | Nichols, B. J., Hall, L., Perry, A. C. F., and Denton, R. M. (1993) Biochem. J. 295, 347-350[Medline] [Order article via Infotrieve] |
10. | Nichols, B. J., Perry, A. C., Hall, L., and Denton, R. M. (1995) Biochem. J. 310, 917-922[Medline] [Order article via Infotrieve] |
11. | Rossman, M. G., Moras, D., and Olsen, K. W. (1974) Nature 250, 194-199[Medline] [Order article via Infotrieve] |
12. | Hurley, J. H., Thorsness, P. E., Ramalingam, V., Helmers, N. H., Koshland, D. E., Jr., and Stroud, R. M. (1989) Proc. Natl. Acad. Sci. U. S. A. 86, 8635-8639[Abstract] |
13. | Hurley, J. H., Dean, A. M., Koshland, D. E., Jr., and Stroud, R. M. (1991) Biochemistry 30, 8671-8678[Medline] [Order article via Infotrieve] |
14. | Stoddard, B. L., Dean, A., and Koshland, D. E., Jr. (1993) Biochemistry 32, 9310-9316[Medline] [Order article via Infotrieve] |
15. | Imada, K., Sato, M., Tanaka, N., Katsube, Y., Matsuura, Y., and Oshima, T. (1991) J. Mol. Biol. 222, 725-738[Medline] [Order article via Infotrieve] |
16. |
Thorsness, P. E.,
and Koshland, D. E., Jr.
(1987)
J. Biol. Chem.
262,
10422-10425 |
17. | Chen, R., Greer, A., and Dean, A. M. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 11666-11670[Abstract] |
18. | Hurley, J. H., Chen, R., and Dean, A. M. (1996) Biochemistry 35, 5670-5678[CrossRef][Medline] [Order article via Infotrieve] |
19. | Doyle, S. A., Fung, S. Y., and Koshland, D. E., Jr. (2000) Biochemistry 39, 14348-14355[CrossRef][Medline] [Order article via Infotrieve] |
20. | Lin, A.-P., McCammon, M. T., and McAlister-Henn, L. (2001) Biochemistry 40, 14291-14301[CrossRef][Medline] [Order article via Infotrieve] |
21. | Lin, A.-P., and McAlister-Henn, L. (2002) J. Biol. Chem. 277, 22476-22483 |
22. | Panisko, E. A. (2000) Subunit Interactions of Saccharomyces cerevisiae NAD+-dependent Isocitrate Dehydrogenase.Doctoral Dissertation , University of Texas Health Science Center, San Antonio, TX |
23. |
Dean, A. M.,
Lee, M. H. I.,
and Koshland, D. E., Jr.
(1989)
J. Biol. Chem.
264,
20482-20486 |
24. | Kuehn, G. D., Barnes, L. D., and Atkinson, D. E. (1971) Biochemistry 10, 3945-3951[Medline] [Order article via Infotrieve] |
25. |
Sikorski, R. S.,
and Hieter, P.
(1989)
Genetics
122,
19-27 |
26. |
Przybyla-Zawislak, B.,
Gadde, D. M.,
Ducharme, K.,
and McCammon, M. T.
(1999)
Genetics
152,
153-166 |
27. | McAlister-Henn, L., and Thompson, L. M. (1987) J. Bacteriol. 169, 5157-5166[Medline] [Order article via Infotrieve] |
28. | Bradford, M. M. (1976) Anal. Biochem. 72, 248-254[CrossRef][Medline] [Order article via Infotrieve] |
29. |
Pace, C. N.,
Vajdos, F.,
Fee, L.,
Grimsley, G.,
and Gray, T.
(1995)
Protein Sci.
4,
2411-2423 |
30. | Lee, P., and Colman, R. F. (2002) Arch. Biochem. Biophys. 401, 81-90[CrossRef][Medline] [Order article via Infotrieve] |
31. | Huang, Y. C., and Colman, R. F. (2002) Biochemistry 41, 5637-5643[CrossRef][Medline] [Order article via Infotrieve] |
32. |
Kornberg, A.,
and Pricer, W. E., Jr.
(1951)
J. Biol. Chem.
189,
123-136 |
33. | Haselbeck, R. J., Colman, R. F., and McAlister-Henn, L. (1992) Biochemistry 31, 6219-6223[Medline] [Order article via Infotrieve] |