Substrate Specificity of Human Endonuclease III (hNTH1)

EFFECT OF HUMAN APE1 ON hNTH1 ACTIVITY*

Dina R. MarensteinDagger , Michael K. ChanDagger , Alvin Altamirano§, Ashis K. Basu§, Robert J. BoorsteinDagger , Richard P. Cunningham, and George W. TeeborDagger ||

From the Dagger  Department of Pathology and the Kaplan Comprehensive Cancer Center, New York University School of Medicine, New York, New York 10016, the § Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269, and the  Department of Biological Sciences, The University at Albany, State University of New York, Albany, New York 12222

Received for publication, December 1, 2002, and in revised form, January 7, 2003

    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Base excision repair of oxidized pyrimidines in human DNA is initiated by the DNA N-glycosylase/apurinic/apyrimidinic (AP) lyase, human NTH1 (hNTH1), the homolog of Escherichia coli endonuclease III (Nth). In contrast to Nth, the DNA N-glycosylase activity of hNTH1 is 7-fold greater than its AP lyase activity when the DNA substrate contains a thymine glycol (Tg) opposite adenine (Tg:A) (Marenstein, D. R., Ocampo, M. T. A., Chan, M. K., Altamirano, A., Basu, A. K., Boorstein, R. J., Cunningham, R. P., and Teebor, G. W. (2001) J. Biol. Chem. 276, 21242-21249). When Tg is opposite guanine (Tg:G), the two activities are of the same specific activity as the AP lyase activity of hNTH1 against Tg:A (Ocampo, M. T. A., Chaung, W., Marenstein, D. R., Chan, M. K., Altamirano, A., Basu, A. K., Boorstein, R. J., Cunningham, R. P., and Teebor, G. W. (2002) Mol. Cell. Biol. 22, 6111-6121). We demonstrate here that hNTH1 was inhibited by the product of its DNA N-glycosylase activity directed against Tg:G, the AP:G site. In contrast, hNTH1 was not as inhibited by the AP:A site arising from release of Tg from Tg:A. Addition of human APE1 (AP endonuclease-1) increased dissociation of hNTH1 from the DNA N-glycosylase-generated AP:A site, resulting in abrogation of AP lyase activity and an increase in turnover of the DNA N-glycosylase activity of hNTH1. Addition of APE1 did not abrogate hNTH1 AP lyase activity against Tg:G. The stimulatory protein YB-1 (Marenstein et al.), added to APE1, resulted in an additive increase in both activities of hNTH1 regardless of base pairing. Tg:A is formed by oxidative attack on thymine opposite adenine. Tg:G is formed by oxidative attack on 5-methylcytosine opposite guanine (Zuo, S., Boorstein, R. J., and Teebor, G. W. (1995) Nucleic Acids Res. 23, 3239-3243). It is possible that the in vitro substrate selectivity of mammalian NTH1 and the concomitant selective stimulation of activity by APE1 are indicative of selective repair of oxidative damage in different regions of the genome.

    INTRODUCTION
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Like its Escherichia coli homolog, endonuclease III (Nth), hNTH11 is a bifunctional DNA N-glycosylase/apurinic/apyrimidinic (AP) lyase that removes ring-saturated pyrimidines, be they hydrated, reduced, or oxidized, from the DNA backbone as the initial step of base excision repair (BER) of such modified residues (1). The oxidation product of thymine, 5,6-dihydroxy-5,6-dihydrothymine (thymine glycol (Tg)) is a widely studied model substrate for hNTH1 and catalyzes its release from DNA via its DNA N-glycosylase activity. This enzyme activity is mediated via formation of a transient Schiff base (imino) enzyme-DNA intermediate, an example of covalent catalysis and a characteristic of all known bifunctional DNA N-glycosylases/AP lyases (4, 5). The Schiff base moiety has been hypothesized to be required for the enzymatic catalysis of the beta -elimination reaction, which effects DNA strand cleavage 3' to the abasic (AP) site formed as a product of the release of the base from the 2'-deoxyribose moiety to which it was linked. The hydrolysis of the Schiff base intermediate can occur in the absence of or following enzyme-catalyzed beta -elimination (AP lyase activity), resulting in DNA strand cleavage. The enzymatic catalysis of beta -elimination by DNA N-glycosylases/AP lyases has been shown to be initiated via abstraction of the deoxyribose pro-S-2'-hydrogen by a basic amino acid in the enzyme active site. Several factors, including pH, can affect the efficiency of the AP lyase step by affecting substrate binding and proton abstraction (5).

Based on the results of studies with Nth, the two activities of hNTH1 (DNA N-glycosylase and beta -elimination catalysis) require the formation of the Schiff base intermediate and were thought to occur concomitantly. However, data from our laboratory (1) and from other laboratories (6-8) indicate that DNA N-glycosylase and beta -elimination catalysis by mammalian members of the endonuclease III enzyme superfamily are not concurrent under the assay conditions employed. We were the first to report that the DNA N-glycosylase and AP lyase activities of hNTH1 are not concurrent, i.e. that the rate of AP lyase-mediated strand cleavage is much slower than the rate of DNA N-glycosylase-mediated base release. These results are similar to the non-concurrence of base release and strand cleavage reported for the mammalian 8-oxoguanine-DNA N-glycosylase homolog OGG1 (8). In this report, we present data demonstrating that the dissociation of the two activities of hNTH1 is dependent on the nature of the orphan base opposite the Tg residue in DNA.

In our studies of the properties of hNTH1, we have focused our attention on protein modulators of hNTH1 activities. This approach resulted from our original observation that the enzyme properties of mammalian endonuclease III homologs are strikingly different from those of bacterial endonuclease III. Our laboratory was the first to identify mammalian endonuclease III homologs and to describe their distinct kinetic properties (9, 10). These observations spurred us to isolate novel proteins that might modulate the enzyme activities of mammalian NTH1. A yeast two-hybrid search resulted in identification of the pluripotent transcription factor YB-1 (Y-box-binding protein-1; also known as DNA-binding protein B) as a stimulator of hNTH1 activity (1).

We now report additional aspects of the proteomics of hNTH1-initiated BER based on the effects of human APE1 (AP endonuclease-1; HAP1/REF1/APE) on hNTH1 activity. APE1, a human homolog of E. coli exonuclease III (Xth), catalyzes the hydrolysis of the phosphodiester bond 5' to AP sites, generating a free 3'-hydroxyl end for the initiation of DNA polymerase repair synthesis.

APE1 has recently been demonstrated to play a significant role in the coordination of BER. APE1 has been shown to modulate the activities of several major DNA N-glycosylases, although physical interaction between APE1 and most of these DNA N-glycosylases has not been demonstrated (6, 7, 11, 12). The functional interaction between APE1 and DNA N-glycosylases is consistent with the model of the coordinated activities of BER enzymes at sites of DNA damage (13-16). Two groups independently demonstrated the stimulation of human OGG1 (hOGG1) by APE1 (6, 7). Both groups demonstrated that APE1 replaces hOGG1 bound to the AP site following base release and prior to hOGG1-catalyzed beta -elimination. This resulted in bypass of the AP lyase step and an increase in hOGG1 turnover. In this study, we report similar findings with hNTH1 and APE1. However, stimulation by APE1 proved to be dependent on the nature of the orphan base opposite the Tg residue in DNA.

    EXPERIMENTAL PROCEDURES
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Proteins-- Expression and purification of recombinant hNTH1 were induced as described previously (10). hNTH1 concentration was quantified using an extinction coefficient of 1 absorbance unit at A410 of 69.4 µM for the C-terminal cubane 4[Fe-S] cluster (17). YB-1 was expressed with a His6 tag in Trichoplusia ni cells and purified as described (1). DNA polymerase beta  was a gift from Dr. Samuel H. Wilson (NIEHS), and APE1 was a gift from Dr. David M. Wilson III (University of California). Formamidopyrimidine-DNA N-glycosylase was purchased from New England Biolabs Inc., and uracil-DNA N-glycosylase was purchased from Invitrogen.

2'-Deoxyribose Oligonucleotides-- The 30-mer 2'-deoxyribose oligonucleotide substrate containing a Tg residue at position 13 (d(GATCCTCTAGAGTgCGACCTGCAGGCATGCA)) was prepared as described (1). The 30-mer 2'-deoxyribose oligonucleotide substrate containing a uracil residue at position 13 of the identical sequence and the complementary 2'-deoxyribose oligonucleotides were synthesized by the Department of Cell Biology of the New York University School of Medicine. 2'-Deoxyribose oligonucleotides were deblocked, deprotected, and purified by 20% denaturing PAGE. The 2'-deoxyribose oligonucleotides were gel-purified and labeled at the 5'-end using [gamma -32P]ATP (PerkinElmer Life Sciences) and T4 polynucleotide kinase (Invitrogen) or at the 3'-end using [alpha -32P]ddATP (Amersham Biosciences) and terminal transferase (New England Biolabs Inc.). All labeled nucleotides were purified by Sephadex G-25 spin column filtration (Amersham Biosciences) and annealed to the complementary strand, which contained an adenine or guanine base opposite the Tg or uracil residue.

Enzyme Assays-- Unless otherwise indicated, assays were performed at 37 °C in buffer containing 50 mM HEPES (pH 7.6), 100 mM KCl, 0.1 mg/ml bovine serum albumin, 5 mM MgCl2, and 1 mM dithiothreitol. Enzyme, protein, and substrate were diluted to working conditions in assay buffer and equilibrated at 37 °C. MgCl2-independent assays were performed with the above reaction buffer minus MgCl2 in the presence of 5 mM EDTA, which permits binding of APE1 to DNA, but inhibits its endonuclease activity (18). Reactions contained 40 nM 32P-5'-end-labeled 2'-deoxyribose oligonucleotide duplex substrate and 5 nM hNTH1 with or without 20 nM APE1. Two sets of 10-µl aliquots were taken at the indicated time periods and snap-frozen in ethanol and dry ice, after which one set was treated with 5 µl of 0.5 M putrescine (pH 8.0) to measure base release. The treated assay mixtures were then heated at 95 °C for 5 min, followed by addition of 15 µl of loading dye (95% deionized formamide, 10 mM EDTA, 0.05% bromphenol blue, and 0.05% xylene cyanol). To measure strand cleavage, samples were treated with an equal volume of loading dye. All samples were then heated at 55 °C for 5 min, and products were separated by 20% PAGE in 7 M urea and 1× Tris borate/EDTA. Low substrate concentration assays were performed with 20 nM hNTH1 and 20 nM 32P-5'-end-labeled 2'-deoxyribose oligonucleotide duplex substrate. At the indicated times, aliquots were taken and treated as described above. Multiple-turnover assays at Vmax were performed individually in 10-µl volumes containing reaction buffer, the indicated concentration of 32P-5'-end-labeled 2'-deoxyribose oligonucleotide duplex substrate, and 20 nM hNTH1 with or without 100 nM YB-1 and/or 100 nM APE1. The reactions were split into two 5-µl aliquots, snap-frozen in ethanol and dry ice, and treated as described above.

To discriminate between endonucleolytic and AP lyase products, reactions were performed using 2'-deoxyribose oligonucleotide duplex substrate labeled at the 3'-end with [alpha -32P]ddAMP. Reactions contained the indicated substrate and enzyme concentrations and were incubated at 37 °C for 30 min. NaBH4 was added to 100 mM, and the reactions were incubated at 37 °C for 20 min for reduction of the 2'-deoxyribose 5'-phosphate moiety (19). The products were purified by Sephadex G-25 spin column filtration prior to separation and analysis.

Single-turnover assays contained 10 nM 2'-deoxyribose oligonucleotide duplex labeled at the 3'-end with [alpha -32P]ddAMP and 100 nM hNTH1 with or without 100 nM APE1 in a volume of 220 µl. Enzyme reaction mixtures were incubated together for 2 min prior to initiation of the assay upon addition of substrate. To measure base release, 5-µl aliquots were removed at the indicated time periods, snap-frozen, and treated with 5 µl of 0.5 M putrescine (pH 8.0). The treated assay mixtures were then heated at 95 °C for 5 min, followed by addition of 10 µl of loading dye. To measure strand cleavage and to determine incision products, 5-µl aliquots were removed at the indicated time periods, snap-frozen, and treated with an equal volume of 0.5 M NaBH4 at 37 °C for 20 min for reduction of the 2'-deoxyribose 5'-phosphate moiety. Samples were then filtered through a Sephadex G-25 spin column, treated with an equal volume of loading dye, and heated at 55 °C for 5 min, and products were separated by 20% PAGE in 7 M urea and 1× Tris borate/EDTA. All products were analyzed quantitatively via phosphorimaging using a Molecular Imager FX system with Quantity One software (Bio-Rad).

Cross-linking of Enzyme to 2'-Deoxyribose Oligonucleotide Substrate (Enzyme Trapping)-- For NaBH4 reduction, assays contained the indicated 32P-5'-end-labeled 2'-deoxyribose oligonucleotide duplex (400 nM) and 20 nM hNTH1 with or without 100 nM APE1 and/or 100 nM YB-1 in a volume of 10 µl. After a 15-min incubation, reaction mixtures were treated with 5 µl of 0.5 M NaBH4 and incubated at 37 °C for 5 min. After addition of an equal volume of Laemmli SDS loading buffer, samples were boiled in preparation for separation and analysis. All samples were separated by 12% SDS-PAGE and analyzed quantitatively via phosphorimaging as described above.

    RESULTS
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Differential Processing of Tg Opposite Adenine and Tg Opposite Guanine by hNTH1-- We previously demonstrated, using a Tg:A-containing substrate, that the initial rate of DNA N-glycosylase-mediated release of Tg by hNTH1 is much greater than the rate of AP lyase-mediated DNA strand cleavage (1). Because all previous assays with Tg have been done with adenine as the opposite base and because this may not be the only context in which Tg occurs in vivo, we decided to look at hNTH1 activity against substrates containing Tg paired opposite guanine (2). Fig. 1 is confirmation of our previous data. The assay was carried out in a shorter reaction period than we previously used and revealed that the difference between the rate of base release and strand cleavage of a Tg:A substrate is even greater than we had reported, differing by an order of magnitude. In sharp contrast, the AP lyase activity of hNTH1 was the same as its DNA N-glycosylase activity against a Tg:G substrate. The DNA N-glycosylase activity of the enzyme against Tg:A appeared to reach Vmax at substrate concentrations >400 nM. However, as we previously reported, hNTH1 does not follow Michaelis-Menten pseudo zero-order kinetics (1). Instead, the apparent kcat (which includes the product release rates) increased with increasing hNTH1 concentrations, suggesting positive cooperativity in hNTH1 substrate processing. This observation and interpretation have been corroborated and expanded upon by Liu and Roy (20). Because of this phenomenon, kcat and Km values for the 2'-deoxyribose oligonucleotide duplex substrates vary with enzyme concentration. As we previously suggested, the V versus S data of Fig. 1 reflect the fact that the enzyme was dissociating from the DNA N-glycosylase-generated AP:A site and initiating base release on new Tg:A substrates without catalyzing beta -elimination (1). However, when the substrate was Tg:G, there was no evidence of enzyme dissociation from AP:G (2).


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Fig. 1.   hNTH1 activity against Tg:A-containing versus Tg:G-containing 2'-deoxyribose oligonucleotide duplex substrates. 20 nM hNTH1 was incubated with the specified concentrations of 32P-5'-end-labeled 2'-deoxyribose oligonucleotide duplex substrate containing either an adenine (circles) or a guanine (squares) opposite the Tg moiety. Open symbols denote DNA N-glycosylase activity determined by putrescine treatment of reaction products. Closed symbols denote AP lyase activity. Mean values were calculated from two independent experiments and did not vary by >10%.

APE1 Increases hNTH1 Processing of Tg:A-containing 2'-Deoxyribose Oligonucleotide Substrates-- We looked at the effect of APE1 on the activities of hNTH1. There is evidence that APE1 interacts with DNA N-glycosylases in BER as part of a "single-nucleotide BER relay" involved in the coordination of processing of BER pathway intermediates (13, 21, 22).

Fig. 2 shows that addition of APE1 increased hNTH1 activity against a Tg:A substrate. The stimulation of hNTH1 activity was independent of the enzyme activity of APE1. The assay mixture lacked MgCl2 and contained sufficient EDTA to abrogate APE1-mediated endonucleolytic cleavage, but not the binding of APE1 to DNA (18). Identical experiments with Tg:G substrates showed no increase in hNTH1 substrate processing in the presence of APE1 (data not shown). We found that the optimum ratio for stimulation of hNTH1 by APE1 varied with both hNTH1 and substrate concentrations, ranging from 1:1 to 1:10. We suggest that this variability was due, at least in part, to the positive cooperativity exhibited by hNTH1 (1, 20).


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Fig. 2.   Effect of APE1 on hNTH1 activity against a Tg:A-containing 2'-deoxyribose oligonucleotide duplex substrate. 40 nM 32P-5'-end-labeled Tg:A-containing 2'-deoxyribose oligonucleotide duplex substrate was incubated with 5 nM hNTH1 with (squares) or without (circles) 20 nM APE1. APE1 endonucleolytic activity was abrogated in the absence of MgCl2 and the presence of 5 mM EDTA. Open symbols denote DNA N-glycosylase activity determined by putrescine treatment of reaction products. Closed symbols denote AP lyase activity.

APE1 Abrogates hNTH1 AP Lyase Activity on Tg:A-containing Substrates, but Not on Tg:G-containing Substrates-- Having determined that the effect of APE1 on hNTH1 activity differed with substrate, we characterized the incision products generated by APE1 and hNTH1 with the two substrates. The incision products generated by hNTH1 in the presence of APE1 in vitro differed with each substrate. Fig. 3 is the gel analysis of the incision products of 32P-3'-end-labeled Tg:A- and Tg:G-containing 2'-deoxyribose oligonucleotide duplex substrates generated by hNTH1 in the presence or absence of APE1. In contrast to the experiments shown in Fig. 2, MgCl2 was added to the reaction to activate the endonucleolytic activity of APE1. The 2'-deoxyribose 5'-phosphate residue generated by APE1-catalyzed endonucleolytic cleavage of an AP site is extremely labile and not retained during electrophoresis. Reduction by NaBH4 prior to electrophoresis stabilizes the sugar moiety and permits size discrimination between 5'-endonucleolytic and 3'-beta -elimination AP lyase products (19). The AP lyase product (p-17-p*ddA) can be seen in lanes containing hNTH1 with either the Tg:A or Tg:G substrate (lanes 2 and 4, respectively). Addition of APE1 to hNTH1 using Tg:A produced a higher molecular weight incision product, which was the endonucleolytic product (p-rAP-p-17-p*ddA, where rAP is reduced apurinic/apyrimidinic) generated by APE1 (lane 3). In the case of the Tg:G substrate, no change in the size of the incision product was detected upon APE1 addition, indicating that the AP lyase activity of hNTH1 was unaffected by APE1 with this substrate (lane 5). In Fig. 3, there are two marker lanes for the beta -elimination product, one with the Tg:G-containing substrate and one with the U:A-containing substrate (lanes 6 and 8, respectively). Formamidopyrimidine-DNA N-glycosylase has beta /delta -elimination activity. Because the substrate was 3'-labeled, the only product observed with formamidopyrimidine-DNA N-glycosylase was the beta -elimination product (lane 6). A second marker lane for the beta -elimination product is the AP site-containing substrate generated by incubation of the U:A-containing substrate with uracil-DNA N-glycosylase and hNTH1 to generate the AP lyase product (p-17-p*ddA) (lane 8). The U:A-containing substrate incubated with uracil-DNA N-glycosylase and APE1 serves as the marker for the endonucleolytic product (p-rAP-p-17-p*ddA) (lane 7).


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Fig. 3.   Effect of APE1 on the incision products generated by hNTH1 in vitro against Tg:A- and Tg:G-containing 2'-deoxyribose oligonucleotide duplex substrates. Reactions contained 10 nM Tg:A- or Tg:G-containing 2'-deoxyribose oligonucleotide duplex substrate labeled at the 3'-end with [32P]ddAMP, 20 nM hNTH1, and 10 nM APE1 as indicated. Lane 6 contained 1 unit of formamidopyrimidine-DNA N-glycosylase (FPG). Another marker lane for the beta -elimination product is also shown with a uracil-containing radiolabeled substrate incubated with uracil-DNA N-glycosylase (UDG) and hNTH1 (p-17-p*ddA, where the asterisk denotes the labeled phosphate). The uracil-containing substrate was incubated with uracil-DNA N-glycosylase and APE1 to generate a marker for the endonucleolytic product (p-rAP-p-17-p*ddA). Reactions were incubated at 37 °C for 30 min prior to addition of NaBH4, followed by analysis as described under "Experimental Procedures."

Incision Products Generated by hNTH1 in the Presence or Absence of APE1, YB-1, and DNA Polymerase beta  Differ in Vitro-- Because our previous work identified YB-1 as a modifier of hNTH1 activity (1), we next investigated whether addition of YB-1 together with APE1 had any effect on the nature of the incision products of hNTH1 with Tg:A-containing 2'-deoxyribose oligonucleotide duplex substrate labeled at the 3'-end with [32P]ddAMP (Fig. 4). Addition of YB-1 in the presence or absence of APE1 increased product formation (lanes 2 and 4). However, it did not change the nature of the incision product, which, in the presence of APE1, was the endonucleolytic product (p-rAP-p-17-p*ddA) (lanes 3 and 4).


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Fig. 4.   Effects of APE1, YB-1, and DNA polymerase beta  on the incision products generated by hNTH1 in vitro against Tg:A-containing 2'-deoxyribose oligonucleotide substrates. Reactions contained 20 mM dNTPs, 16.6 nM Tg:A-containing 2'-deoxyribose oligonucleotide duplex substrate labeled at the 3'-end with [32P]ddAMP, 16.6 nM hNTH1, 8.3 nM APE1, 8.3 nM DNA polymerase beta  (beta -pol), and 83 nM YB-1 as indicated. Lane 9 contained 1 unit of formamidopyrimidine-DNA N-glycosylase (Fpg) as a marker for the beta -elimination product (p-17-p*ddA, where the asterisk denotes the labeled phosphate). Reactions were incubated at 37 °C for 30 min prior to addition of NaBH4, followed by analysis as described under "Experimental Procedures."

Because hNTH1 did not demonstrate AP lyase activity against Tg:A sites in the presence of APE1, we investigated whether the removal of the 2'-deoxyribose 5'-phosphate residue could be effected by DNA polymerase beta . Fig. 4 demonstrates the reappearance of the beta -elimination product upon addition of DNA polymerase beta  to hNTH1 and APE1 with a Tg:A substrate (lane 7). This observation is consistent with the known 2'-deoxyribose 5'-phosphatase activity of DNA polymerase beta  (22). The marker lane for the beta -elimination product (p-17-p*ddA) contains formamidopyrimidine-DNA N-glycosylase (lane 9). Similar experiments with an N-terminal deletion mutant of hNTH1 lacking amino acids 1-57 produced the same results in the presence of APE1 and YB-1 (data not shown), suggesting that the effects of YB-1 and APE1 are not mediated by the N terminus of hNTH1.

APE1 Does Not Increase the DNA N-Glycosylase Activity of hNTH1 under Conditions of Single Turnover-- To elucidate the mechanism by which APE1 affects hNTH1 activity, we investigated whether APE1 changed the catalytic properties of hNTH1 or whether it affected product inhibition. To address this, we conducted the reaction under single-turnover conditions in which [E] [S] using substrates labeled at the 3'-end with [32P]ddAMP to determine the nature of the incision product. Because all substrate molecules are bound by enzyme under single-turnover conditions, the rates that determine product formation are dependent only on the rate of base release (Schiff base formation) and beta -elimination (24). If APE1 did not affect either of these catalytic properties, then there should be no stimulation under conditions of single turnover.

Fig. 5 illustrates that no stimulatory effect of DNA N-glycosylase activity was observed under conditions in which neither enzyme turnover nor substrate binding contributed significantly to the rate of the reaction. A complete abrogation of the AP lyase-mediated AP site cleavage was seen with the Tg:A substrate (Fig. 5A). This is consistent with the results of Figs. 3 and 4 (lanes 3).


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Fig. 5.   Representative plots of the effect of APE1 on the single-turnover kinetics of hNTH1 with Tg:A-containing (A) and Tg:G-containing (B) 2'-deoxyribose oligonucleotide duplex substrates. Reactions contained 10 nM Tg:A-containing (A) or Tg:G-containing (B) 2'-deoxyribose oligonucleotide duplex substrate labeled at the 3'-end with [32P]ddAMP and 100 nM hNTH1 with (squares) or without (circles) 100 nM APE1. Open symbols denote DNA N-glycosylase activity determined by putrescine treatment of reaction products. Closed symbols denote AP lyase activity. Reactions were incubated at 37 °C for the indicated time periods, after which aliquots were stopped by snap-freezing. One set was treated with putrescine and the other with NaBH4. Analysis was as described under "Experimental Procedures."

Interestingly, in the case of the Tg:G substrate, addition of APE1 increased the rate of hNTH1 AP lyase activity (Fig. 5B). We previously mentioned that addition of APE1 did not result in increased product formation by hNTH1 under turnover conditions using Tg:G (see above). Thus, the results of Fig. 5B suggest that hNTH1 remained tightly bound to the nicked AP:G DNA, which is the product of AP lyase activity.

With both Tg:A and Tg:G substrates, the binding to the substrate and base release were rapid, whereas beta -elimination-catalyzed cleavage of the resulting AP site was clearly the rate-limiting reaction. Under single-turnover conditions, the lag of the AP lyase step behind the DNA N-glycosylase step was only slightly longer for Tg:A than for Tg:G, indicating that the difference in processing of the two substrates was due to enzyme turnover determined by the relative rates of dissociation of hNTH1 from its products (Fig. 5). Further evidence for this is shown in Fig. 6, in which both hNTH1 and Tg:A and Tg:G substrates were present at low equimolar concentrations. Under these conditions, the dissociation of the DNA N-glycosylase and AP lyase activities of hNTH1 was apparent against the Tg:A substrate, but there was no dissociation between the two activities against the Tg:G substrate.


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Fig. 6.   Representative plot of product formation by hNTH1 against Tg:A- and Tg:G-containing 2'-deoxyribose oligonucleotide duplex substrates as a function of time under multiple-turnover conditions. Reactions contained 20 nM 32P-5'-end-labeled Tg:A-containing (circles) or Tg:G-containing (squares) 2'-deoxyribose oligonucleotide duplex substrate as indicated with 20 nM hNTH1. Open symbols denote DNA N-glycosylase activity determined by putrescine treatment of reaction products. Closed symbols denote AP lyase activity.

APE1 Stimulates hNTH1 Activity under Conditions of Vmax-- To determine whether APE1 increased the maximal rate of turnover for the enzyme, we tested stimulation by APE1 under conditions of Vmax for both DNA N-glycosylase and AP lyase activities using 20 nM hNTH1 and 400 nM Tg:A substrate, identical to the conditions of Fig. 1. However, in the experiments of Fig. 7, MgCl2 was added to activate APE1 endonucleolytic activity. In reactions containing hNTH1 alone or with YB-1, DNA cleavage was due solely to hNTH1-catalyzed beta -elimination. For all four sets of experiments, DNA N-glycosylase activity was quantified by measuring the cleavage of hNTH1-generated AP sites by treatment with organic base (putrescine), which effects cleavage via beta -elimination (25). In reactions containing APE1, all cleavages were the result of APE1 activity. Addition of APE1 resulted in a 2-fold increase in the number of hNTH1-generated AP sites compared with hNTH1 alone. Addition of YB-1 also caused a 2-3-fold increase in DNA N-glycosylase activity, as we previously described (1). Like YB-1, APE1 increased the kcat of hNTH1 (as a DNA N-glycosylase) under conditions of substrate excess. The last experiment of this group indicates that the effects of APE1 and YB-1 were additive, resulting in a close to 4-fold increase in product. This combined stimulation could actually be much greater because, under our experimental conditions, most of the substrate had been processed within 15 min.


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Fig. 7.   Effect of APE1 on hNTH1 activity against a Tg:A-containing 2'-deoxyribose oligonucleotide duplex under conditions of Vmax. Reactions consisted of 20 nM hNTH1 incubated with 400 nM 32P-5'-end-labeled Tg:A-containing 2'-deoxyribose oligonucleotide duplex substrate with 100 nM YB-1 and/or 100 nM APE1 as indicated for a reaction time of 15 min. Black bars denote DNA cleavage either by hNTH1-catalyzed AP lyase activity (in the absence of APE1) or APE1 endonucleolytic activity (in the presence of APE1). Gray bars represent reaction products treated with putrescine. In the absence of APE1, these denote hNTH1 DNA N-glycosylase activity. In the presence of APE1, all AP sites generated by hNTH1 DNA N-glycosylase activity were endonucleolytically cleaved by APE1. Means ± S.D. were calculated from three independent experiments.

Corroborating our results of experiments performed under low Tg:G substrate concentrations, addition of APE1 to hNTH1 using substrate under conditions of Vmax (400 nM Tg:G) did not result in increased product formation (data not shown). Interestingly, hNTH1 activity against Tg:G was stimulated by addition of YB-1, but there was no additive effect with APE1 (data not shown).

APE1 Addition Decreases the Half-life of Covalent Enzyme-Substrate Complexes Formed by hNTH1 with Its Substrate-- To investigate the effect of APE1 on the reaction intermediates of hNTH1 with its substrate, we used the reducing agent NaBH4, to trap the covalent enzyme-substrate intermediate (Fig. 8). NaBH4 is a strong reducing agent, capable of reducing the Schiff base as well as existing AP sites (26). Therefore, the trapping of the covalent reaction intermediate with NaBH4 (Fig. 8) revealed the number of covalent complexes present in the reaction mixture at a given time point, in this case, 15 min. For the Tg:A substrate under steady-state conditions, addition of APE1 resulted in a decrease in the steady-state number of complexes initiated by hNTH1 15 min after the start of the reaction when the rate of product formation was still linear with time. There was no significant change in the number of steady-state complexes with the Tg:G substrate upon APE1 addition. Addition of YB-1 resulted in a greater number of complexes with both substrates, corroborating our previous data (Ref. 1 and data not shown). The marked reduction in the number of enzyme-substrate complexes (Tg:A) in the presence of both YB-1 and APE1 is a reflection of the increased stimulation of hNTH1 turnover, resulting in almost total substrate processing within 15 min. These data suggest that APE1 decreased the half-life of the enzyme-substrate covalent intermediate, thereby stimulating dissociation of hNTH1 from the AP:A site without catalyzing beta -elimination, consistent with results of other laboratories studying the effect of APE1 on hOGG1 (6, 7).


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Fig. 8.   Representative plot of the effect of APE1 and YB-1 on steady-state levels of the hNTH1 Schiff base intermediate with a Tg-containing 2'-deoxyribose oligonucleotide duplex. The covalent enzyme-substrate intermediate was trapped using NaBH4. Reactions contained 400 nM 32P-5'-end-labeled Tg:A-containing (black bars) or Tg:G-containing (striped bars) 2'-deoxyribose oligonucleotide duplex substrate with 20 nM hNTH1, 100 nM YB-1, and/or 100 nM APE1 as indicated. Reactions were incubated at 37 °C for 15 min; NaBH4 was then added to a concentration of 100 mM, and the reactions were incubated for another 2 min at 37 °C prior to addition of Laemmli SDS sample buffer.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The generation of Tg in DNA occurs through oxidative attack on the pyrimidine bases. Tg opposite adenine can be formed by direct hydroxyl radical attack under aerobic conditions on thymine in situ opposite its correctly paired base. As a blocker of both DNA and RNA polymerases, Tg opposite adenine is a cytotoxic lesion (27). When Tg is bypassed by a DNA polymerase, adenine is usually incorporated opposite Tg (27). Because the 2'-deoxynucleotide triphosphate of Tg has been shown to be a poor substrate for polymerase incorporation, formation of Tg:G is unlikely to be the result of insertional mispairing (28). Thus, the formation of Tg:G is due primarily to direct oxidative attack on 5-methylcytosine, resulting in deamination and oxidation, as we previously demonstrated (3). This modification is likely to occur in CpG islands, which are rich in 5-methylcytosine and play a role in the regulation of gene expression. In contrast to Tg:A, Tg:G is potentially mutagenic because, if unrepaired and bypassed by a DNA polymerase, the insertion of adenine opposite Tg would lead to a CG right-arrow TA transition mutation as well as a disruption in methylation patterns (3).

We further investigated the mechanism of the difference in hNTH1 activities against Tg when it is paired with a different orphan base. Although hNTH1 exhibited marked dissociation between its DNA N-glycosylase and AP lyase activities when the substrate was Tg:A, the enzyme demonstrated concomitant DNA N-glycosylase and AP lyase activities when the substrate was Tg:G. Although the rates for base release by the enzyme were much greater for Tg:A than for Tg:G, the rates for AP lyase activity initiated by hNTH1 against Tg:A and Tg:G were nearly identical. Our data indicate that this discrepancy was due to the presence or absence of hNTH1 turnover rather than inherent differences in reaction rates.

Furthermore, we have reported that APE1 stimulated hNTH1 activity against Tg:A by abrogation of the AP lyase step, presumably by effecting the dissociation of hNTH1 from the AP site, thereby increasing the turnover of the DNA N-glycosylase activity of hNTH1 (Fig. 9A). Thus, in the presence of APE1, hNTH1 essentially becomes a monofunctional DNA N-glycosylase against Tg:A, but not against Tg:G. Similar results have been reported for hOGG1 (6, 7). Although we did not observe stimulation of hNTH1 product formation by APE1 with Tg:G under turnover conditions, we did observe APE1 stimulation of hNTH1-catalyzed beta -elimination with Tg:G under single-turnover conditions (Fig. 5B). Taken together, these data suggest that hNTH1 remains bound to the nicked AP site product. This indicates that APE1 did not mitigate the inhibition of hNTH1 by the nicked DNA product of beta -elimination of the AP:G site (Fig. 9B). Although we had previously suggested that Schiff base hydrolysis (k4) and product release (k5) are not rate-limiting (1), our current data suggest the opposite. The observation that YB-1 did stimulate Tg:G substrate processing by hNTH1 suggests that YB-1 may effect product release as well as the steady-state equilibrium between the Schiff base enzyme-substrate intermediate and the noncovalent enzyme-substrate intermediate.


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Fig. 9.   Kinetic scheme for the effect of APE1 on hNTH1 activity against Tg:A-containing (A) and Tg:G-containing (B) 2'-deoxyribose oligonucleotide duplex substrates. S1 is the substrate with modified base (i.e. Tg) intact, and S2 is the substrate containing an AP site after base release. P1 is the 3'-end of the beta -eliminated 2'-deoxyribose oligonucleotide, and P2 is the 5'-end of the beta -eliminated 2'-deoxyribose oligonucleotide. The double bond indicates the Schiff base between the enzyme and substrate. k2 is the rate of base release. k3 is the rate of beta -elimination. k4 is the rate of Schiff base hydrolysis. k-6 and k6 are the steady state of Schiff base resolution. k-7 and k7 are the binding and release of the noncovalently bound AP site moiety, respectively. A, the inhibition of k3 by APE1 drives the reaction toward k6/k7, the dissociation of hNTH1 from the AP:A substrate, resulting in increased turnover, a faster reaction rate, and a shorter half-life for the covalent enzyme-substrate intermediate. This leaves an AP site ready for processing by APE1 and repair synthesis. B, the effect of APE1 on k3 results in an increased rate of AP lyase activity. However, there is no evidence that hNTH1 is driven to dissociate from the beta -elimination product, and turnover is not significantly affected.

What is a possible mechanistic reason for the differential activity of hNTH1 acting on Tg:A versus Tg:G? A detailed in vitro analysis of hNTH1 substrate processing by Eide et al. (29) revealed that the beta -elimination activity of hNTH1 is strongly dependent upon the nature of the orphan base pairing with an AP site, being 100-fold greater with AP:G compared with AP:A. Thus, the difference in hNTH1 processing of Tg:A versus Tg:G may be a reflection of the affinity of hNTH1 for AP:G versus AP:A. From our turnover data (Figs. 1 and 6), we conclude that the AP lyase activity of hNTH1 against the AP site-containing substrate is dependent on the binding affinity of hNTH1 for the AP site. Thus, the dissociation of hNTH1 from the AP:A substrate following the enzymatic release of Tg suggests a low binding affinity of hNTH1 for the AP:A site. This is shown in the kinetic mechanism outlined in Fig. 9A, where k6 represents the hydrolysis of the Schiff base without catalysis of beta -elimination and k7 represents the dissociation of the enzyme from the AP site. Our data suggest that APE1 drives the steady-state equilibrium from the covalent (Schiff base, E=S2) to the noncovalent (ES2) intermediate between hNTH1 and its AP:A substrate, resulting in the dissociation (E + S2) of hNTH1 from the AP:A site. The higher affinity of hNTH1 for AP:G is depicted by virtually unobservable values for k6 and k7, in which case, all Schiff base formation results in catalysis of beta -elimination and formation of AP lyase product (E=P2) (Fig. 9B). Because APE1 stimulates hNTH1 AP lyase activity with Tg:G without stimulating turnover, we suggest that hNTH1 remains tightly bound to its AP lyase product (E=P2).

The different activities that we observed with hNTH1 in vitro may be indicative of the involvement of different substrate-dependent interacting and modifying proteins in vivo. Certain lesions, by virtue of their mutagenic or toxic characteristics, may be preferentially channeled down a particular BER pathway, i.e. long- or short-patch BER. It has been suggested that the intrinsic properties of the DNA N-glycosylase involved in the initial recognition and removal of the damaged base may be responsible for BER pathway selection (30). In this case, the intrinsic properties may include the pairing base discrimination exhibited by hNTH1.

The in vivo significance of AP lyase activity is poorly understood. The removal of the damaged base by the DNA N-glycosylase generates an AP site, which is a labile and therefore dangerous intermediate for the cell. AP sites are the universal intermediates for all BER, and it is postulated that most of these sites are processed by APE1 (31). Our studies with hNTH1 are consistent with studies performed with mammalian OGG1, which suggested that AP site processing is the rate-limiting step in BER initiated by bifunctional DNA N-glycosylases/AP lyases (8). As we previously proposed, severe oxidative stress or UV irradiation would result in the formation of a large number of damaged bases throughout cellular DNA (1). In theory, the spontaneous removal of the damage via concomitant DNA N-glycosylase/AP lyase activity by bifunctional DNA N-glycosylases/AP lyases would result in the simultaneous formation of a large number of DNA strand breaks, a cytotoxic intermediate. In support of this hypothesis, recent data on H2O2 sensitivity in E. coli identified the intermediates of Tg repair, rather than the persistence of Tg, as significant contributors to cell death (32). The delayed AP lyase activity and product inhibition inherent in hNTH1 activity may allow for the regulation of AP site processing. The substrate-specific product inhibition exhibited by hNTH1 may also represent the channeling of AP site processing into specific BER pathways in different regions of the genome. The rate of removal of Tg from cellular DNA has been shown to be very slow compared with the removal of uracil or the repair of AP sites (22, 33). Repair of Tg has been reported to consist mostly of short-patch BER involving DNA polymerase beta  (33, 34). Our data suggest that it is the AP lyase activity of DNA polymerase beta  that is responsible for the removal of the 2'-deoxyribose 5'-phosphate residue left by hNTH1-catalyzed base release, followed by APE1-catalyzed hydrolysis of the 5'-phosphate bond. The fact that DNA polymerase beta  is the primary polymerase involved in the major Tg removal pathway supports this model (34, 35). The pivotal role played by APE1 physically connects the activities of the initial BER factors to the DNA repair synthesis enzymes, such as DNA polymerase beta , FEN1 (Flap endonuclease-1), and DNA ligase (13, 21, 31). Data from our laboratory (1) and others (6, 7, 36-39) have shown that interactions between BER enzymes and other proteins can stimulate damage recognition or excision, suggesting the probability of multiprotein complex formation in the initial events of BER.

APE1 is not limited to its role in BER, but is a composite of the different activities of the enzyme as a redox factor (40). Notably, APE1 has been shown to control the activity of p53 through redox alteration; and in turn, p53 has been shown to play a role in BER (40-44). Like YB-1, APE1 has been shown to be a pluripotent factor and may be a key effector of the relationships between mammalian BER, oxidative signaling, transcription regulation, and cell cycle control (40, 45).

We previously demonstrated that YB-1 affects hNTH1 activity via the steady-state equilibrium between the covalent (E = S2) and noncovalent (ES2) enzyme-DNA intermediate (1). We proposed that this equilibrium may be a checkpoint for modulation of hNTH1 activity (1). In this study, we have shown that APE1 modulated hNTH1 activity at the same equilibrium point, albeit to a different effect. Thus, although YB-1 and APE1 seem to modulate hNTH1 activity in opposite ways, they have a synergistic effect in vitro. The overall effect of these two factors on hNTH1 activity is to increase hNTH1 turnover and substrate processing. Whether this has significance in vivo remains to be determined.

In conclusion, we have performed further analyses into the mechanism of hNTH1 activity and identified substrate-specific enzyme properties. We have also demonstrated a functional interaction between hNTH1 and the major mammalian AP endonuclease, APE1. This interaction is dependent on the nature of the orphan base opposite Tg.

We recently reported (2), as has another laboratory (46), that NTH1-/- mice are viable and exhibit no abnormal phenotype. Extracts of tissues of NTH1-/- mice express an enzyme activity that cleaves Tg:G substrates more rapidly than Tg:A substrates. This pattern seems to be in direct contrast to the substrate selectivity of hNTH1. Whether the substrate selectivity of NTH1 is a reflection of actual differences in NTH1-mediated in vivo processing of Tg:A versus Tg:G and whether the selective stimulatory effects of APE1 affect processing await further elucidation.

    ACKNOWLEDGEMENTS

We thank Dr. David M. Wilson III for supplying APE1 and Dr. Samuel H. Wilson for supplying DNA polymerase beta .

    FOOTNOTES

* This work was supported by the Department of Pathology, New York University School of Medicine, and an award from the New York University School of Medicine Research Bridging Support Fund (to G. W. T.) and by NIEHS Grant ES 09127 (to A. K. B.) and NIH Grant GM 46312 (to R. P. C.) from the National Institutes of Health.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| To whom correspondence should be addressed: Dept. of Pathology, New York University Medical Center, 550 First Ave., New York, NY 10016. Tel.: 212-263-5473; Fax: 212-263-8211; E-mail: george.teebor@med.nyu.edu.

Published, JBC Papers in Press, January 8, 2003, DOI 10.1074/jbc.M212168200

    ABBREVIATIONS

The abbreviations used are: hNTH1, human NTH1; AP, apurinic/apyrimidinic; BER, base excision repair; Tg, thymine glycol; hOGG1, human OGG1.

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TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

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