In Situ Conversion of Protochlorophyllide b to Protochlorophyllide a in Barley

EVIDENCE FOR A NOVEL ROLE OF 7-FORMYL REDUCTASE IN THE PROLAMELLAR BODY OF ETIOPLASTS*

Steffen ReinbotheDagger §, Stephan Pollmann§, and Christiane Reinbothe||

From the Dagger  Université Joseph Fourier et CNRS, UMR 5575, BP53, CERMO, F-38041 Grenoble cedex 9, France, the § Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, Universitätsstraße 150, D-44801 Bochum, and the  Lehrstuhl für Pflanzenphysiologie, Universität Bayreuth, Universitätsstraße 30, D-95447 Bayreuth, Germany

Received for publication, September 23, 2002, and in revised form, October 15, 2002

    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

We recently put forth a model of a protochlorophyllide (Pchlide) light-harvesting complex operative during angiosperm seedling de-etiolation (Reinbothe, C., Lebedev, N., and Reinbothe, S. (1999) Nature 397, 80-84). This model, which was based on in vitro reconstitution experiments with zinc analogs of Pchlide a and Pchlide b and the two NADPH:protochlorophyllide oxidoreductases (PORs), PORA and PORB, of barley, predicted a 5-fold excess of Pchlide b, relative to Pchlide a, in the prolamellar body of etioplasts. Recent work (Scheumann, V., Klement, H., Helfrich, M., Oster, U., Schoch, S., and Rüdiger, W. (1999) FEBS Lett. 445, 445-448), however, contradicted this model and reported that Pchlide b would not be present in etiolated plants. Here we demonstrate that Pchlide b is an abundant pigment in barley etioplasts but is rather metabolically unstable. It is rapidly converted to Pchlide a by virtue of 7-formyl reductase activity, an enzyme that had previously been implicated in the chlorophyll (Chl) b to Chl a reaction cycle. Our findings suggest that etiolated plants make use of 7-formyl reductase to fine tune the levels of Pchlide b and Pchlide a and thereby may regulate the steady-state level of light-harvesting POR-Pchlide complex.

    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Angiosperms have developed sophisticated mechanisms to harvest sunlight and to convert this into various physiological responses (1). They make use of various photoreceptors, such as the red/far red light-absorbing phytochromes, the blue light-absorbing cryptochromes, and the blue light-absorbing phototropins, to adapt to different light qualities and quantities and to sense the direction and duration of incident light (for a review, see Ref. 2). All of these photoreceptors are chromoproteins, which undergo characteristic spectral changes upon illumination.

Another blue and red light-absorbing protein-pigment complex is the protochlorophyllide (Pchlide)1 holochrome (3). It is localized in the prolamellar body of etioplasts. These plastids form when angiosperms germinate in darkness. The entire developmental process of seedling germination leading to prolamellar bodies is termed skotomorphogenesis or etiolation (1). In plants displaying an hypogeic type of germination, it takes place underneath the soil. The newborn seedlings then utilize all nutrient reserves contained in the seed to bring the cotyledons above the soil.

Previous work has shown that the Pchlide holochrome is a higher molecular mass complex of about 600 kDa (3). More recent work suggested that it may be composed of galacto- and sulfolipids (4), Pchlide (5, 6), and an enzyme called the NADPH:Pchlide oxidoreductase (POR; EC 1.3.33.1) (7, 8). It was discovered that two distinct forms of POR exist in barley etioplasts, called PORA and PORB (9, 10). Moreover, two species of Pchlide have been distinguished in isolated prolamellar bodies by low temperature in situ fluorescence measurements: Pchlide 628/632 (the first number indicates the absorption maximum, the second the respective fluorescence emission maximum at the chosen excitation wavelength) and Pchlide 650/657 (for a review, see Ref. 11). Whereas the former remained quantitatively unchanged upon illumination with a single, 1-ms flash of white light, the latter was readily converted to Chlide 684/690. This differential behavior led scientists to name Pchlide 628/632 photoinactive and Pchlide 650/657 photoactive (summarized in Ref. 11). Both before and after flash light illumination, energy transfer was observed, taking place from photoinactive Pchlide to photoactive Pchlide in etiolated plants and from photoinactive Pchlide to Chlide in preflashed plants (12-17).

To resolve all of these puzzling previous observations, we proposed a model of a "light-harvesting POR-Pchlide" complex, named LHPP (18). Based on in vitro reconstitution experiments with synthetic zinc analogs of Pchlide, we put forth the idea that LHPP may be composed of 5 PORA-Pchlide b-NADPH ternary complexes and 1 PORB-Pchlide a-NADPH ternary complex embedded into the lipid bilayers of the prolamellar body of etioplasts (18) (see also Ref. 19 for a summary).

A particularly important question that had thus far remained unanswered was whether Pchlide b implicit in the LHPP model would be present in etiolated barley plants. Whereas previous work had indicated that Pchlide b is present in green plants (20), no comparable study had thus far been available reporting the identification of Pchlide b in etiolated plants, where the pigment, according to our in vitro reconstitution experiments (18), should be found in maximum levels. In a recent paper, Scheumann et al. (21) even generally questioned the existence of Pchlide b, but at the same time demonstrated that barley etioplasts rapidly convert exogenously added zinc protopheophorbide b (ZnPPb) to ZnPPa. This prompted us to conclude that Chl(ide) b reductase, presumably responsible for this conversion (22-26), could also metabolize the endogenously occurring Pchlide b to Pchlide a.

In the present study, we readdressed the experimental design of Scheumann et al. (21). We demonstrate that Chl(ide) b reductase (which may alternatively be named 7-formyl reductase; see below) is indeed able to convert Pchlide b to Pchlide a in situ. This reaction already occurs upon plastid lysis and subsequent detergent solubilization of isolated prolamellar bodies. Both experimental steps lead to a denaturation of the prolamellar body and the release of the PORA and make Pchlide b readily accessible to 7-formyl reductase. Our results provoke the idea that 7-formyl reductase may be involved in fine tuning the levels of Pchlide b and Pchlide a in etioplasts.

    MATERIALS AND METHODS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Pigments-- All glassware used throughout this study was pretreated with diethyl pyrocarbonate (DEP). This compound had previously been shown to inhibit bacterial and plant Rieske-type oxygenases (27, 28), to which Chlide a oxygenase and related enzymes belong (29, 30). To block this activity seemed particularly necessary, in order to allow accurate determination of Pchlide a and Pchlide b levels, respectively.

Pigments were extracted from intact barley etioplasts as described herein and in Ref. 31. Separation by HPLC was performed on a C18 reverse phase silica gel column (Macherey-Nagel Co., 250 × 4.6 mm, Nucleosil ODS 5 µm) as described in Ref. 32. Either a step gradient was used, starting with 34% 25 mM aqueous ammonium acetate, 15% acetone, and 51% methanol (buffer A), increasing to 16% H2O, 60% acetone, and 24% methanol within 20 min (buffer B), and finally to 100% acetone another 4 min later, or linear gradients from buffer A to buffer B. Absorbance measurements were made at 455 nm, which corresponds to the Soret band of Pchlide b, to detect and quantify Pchlide a and Pchlide b levels. At this wavelength, the extinction coefficients of Pchlide b and Pchlide a are 5-fold different (21). As internal standards, we used synthetic Pchlides a and b, which were prepared from Chlides a and b with an excess of 2,3-dichloro-5,6-dicyanobenzoquinone as described in Refs. 21 and 32. At a flow rate of 1 ml/min, Pchlide a has a retention time of ~15 min, and Pchlide b has a retention time of ~12.5 min. For simultaneous separation of Pchlide a and Pchlide b and their reduced products (i.e. Chlides a and b, respectively), a C30 reverse phase column (250 × 4.6 mm, 5 µm; YMC Inc., Willmington, NC) (33) was used. HPLC was performed in a Varian ProStar model 410 apparatus, a ProStar model 240 pump, and a ProStar 330 photodiode array detector, essentially as described in Ref. 33 (see accompanying paper (31) for details). In some experiments, a combination of octadecyl silica and poly(ethylene) powder media was used, likewise allowing separation of both porphyrins and chlorins in the same HPLC run (34).

Chemical synthesis of ZnPPa and ZnPPb and their binding to isolated prolamellar body membranes was performed as described in Ref. 32. 7-Hydroxy-Pchlide a was synthesized as described herein. Liquid secondary ion mass spectrometry was performed in an m-nitrobenzyl alcohol matrix with a Finnigan model MAT900 and a cesium gun (20 kV, 1 mA) according to Schoch et al. (32).

Preparation and Solubilization of Prolamellar Bodies-- Etioplasts were prepared from etiolated barley plants by Percoll density gradient centrifugation as described previously (35, 36). For low temperature analyses at 77 K (see below) and pigment measurements (see above), the etioplast suspension was directly used. Plastids to be lysed and further subfractionated were diluted with the buffer described by Li et al. (37). After sedimentation, the latter plastids were fractionated into prolamellar bodies, prothylakoids, and stroma, on discontinuous step gradients of sucrose (38). n-Octyl-beta -D-glucoside treatment of isolated prolamellar bodies was performed as described in Ref. 21. After solubilization, the assay mixtures were centrifuged, and the resulting supernatant and membrane fractions were analyzed separately as specified herein.

Protein and Pigment Analyses-- Etioplasts prepared on a Percoll gradient were extracted with an 100-fold excess of either 100% acetone containing 0.1% DEP or only 80% acetone lacking DEP. By analogy, plastid subfractions obtained as described above were treated identically. Protein was recovered by centrifugation, washed several times with ethanol and ether, and run by SDS-PAGE on 10-20% (w/v) polyacrylamide gradients, whereas pigments found in the corresponding supernatant fractions were directly used for fluorescence measurements (see below).

Immunodecoration of electrophoretically resolved proteins was performed using an ECL Western blotting analysis system (Amersham Biosciences) and an anti-POR-specific antiserum (9).

Spectroscopic Analyses-- Low temperature fluorescence measurements were performed at 77 K at an excitation wavelength of 440 nm (39), in a spectrometer LS50B (PerkinElmer Life Sciences).

    RESULTS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Previous studies had shown that barley and cucumber etioplasts contain enzyme activity that converts Chl b to Chl a (22). By analogy, this enzyme activity was found to also convert the nonesterified precursor of Chl b, Chlide b, as well as pyrochlorophyllide b and the magnesium-free pheophorbide b into the respective Chl a and 7-hydroxy compounds (23-26).

To test whether this enzyme activity, which we tentatively named 7-formyl reductase to indicate this broad substrate specificity, would also be able to convert Pchlide b to Pchlide a in situ, we followed the experimental design of Scheumann et al. (21). Briefly, intact barley etioplasts were isolated on a Percoll gradient, sedimented by centrifugation, lysed, and incubated with ZnPPb, the zinc analog of Pchlide b (32). As a control, barley etioplasts were left intact (26) and incubated identically. Mock incubations lacking ZnPPb were conducted in parallel.

Two different pigment extraction procedures were used. In the first case, we used an almost pure, nonaqueous solution of acetone containing 0.1% DEP, which was used in order to block the potential generation of Pchlide b by virtue of the previously identified Chlide a oxygenase and related enzymes (27-30). This solution is referred to as 100% acetone throughout the rest of the paper. In the second case, an aqueous, non-DEP-supplemented solution containing only 80% acetone was used, as reported in Ref. 21. Pigment analyses were made by HPLC, using a photodiode array detector. As shown previously (21, 32), this allowed simultaneous separation and identification of pigments during the actual HPLC run.

When etiolated leaf material was extracted with 100% acetone, HPLC analyses revealed the existence of three main porphyrin species, eluting at 11 min (peak 1), 12.5 min (peak 2), and 15 min (peak 3), respectively (Fig. 1A). Depending on the steepness of the solvent gradient and actual flow rate, some variations in the retention times were seen (see also Fig. 4). The relative peak intensities, however, were maintained. This at first glance indicated that the pigments contained in peak 2 were more abundant than those resolved in peaks 1 and 3 (Fig. 1A). By contrast, when replicate etioplast samples were extracted with only 80% acetone (21), the level of pigment in peak 3 seemed to largely exceed those contained in the other fractions (Fig. 1B). Only traces of peak 2 were seen, and peak 1 remained even below the level of detection.


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Fig. 1.   Detection of pigments in etiolated barley plants. Pigments were extracted from dark-grown barley plants either with 100% acetone containing 0.1% (v/v) DEP (A) or an aqueous, non-DEP-supplemented 80% (v/v) solution of acetone (B). The extracts were separated by HPLC, and porphyrins were identified by absorbance measurements at 455 nm (see "Materials and Methods").

Absorbance profiles of the resolved pigments are shown in Fig. 2. They demonstrated that the pigments contained in peak 2 had a main absorption maximum at 448 nm and two lower maxima at 578 and 622 nm, respectively (Fig. 2B). These corresponded to values reported previously for Pchlide b: the so-called Soret band (448 nm), the Qx band (578 nm), and the Qy band (622 nm) (21). The Qx band had a higher absorbance than the Qy band, which is a typical feature of all investigated pigments of the proto b series (21, 32).


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Fig. 2.   Absorbance profiles of HPLC-separated pigments. Pigments were extracted and separated by HPLC as described in Fig. 1. Absorption spectra were recorded between 400 and 750 nm for pigments recovered from peak 1 (A), peak 2 (B), and peak 3 (C), respectively. Main absorption maxima are indicated.

For the pigments contained in peak 3, a main absorption maximum at 438 nm and a second, lower band at 628 nm were observed (Fig. 2C). These maxima are characteristics of Pchlide a (5, 7). The absorption spectrum of pigments contained in peak 1 was similar to that of the pigments resolved in peak 3; however, the minor peak seemed slightly blue-shifted (Fig. 2A), suggesting the presence of 7-OH-Pchlide a (21).

To prove the identity of the various compounds, synthetic standards were prepared. 7-Hydroxy-Chl a, Chl b, and Chl a were used as educts in a combined enzymatic and chemical procedure (21, 32). In the first step, the phytol chain was removed by the chlorophyllase reaction (40). In the second step, the double bond in ring D of the macrocycle was reestablished by chemical dehydrogenation with 2,3-dichloro-5,6-dicyanobenzoquinone (21, 32).

Fig. 3A shows absorption spectra of Chl a, Chl b, and 7-hydroxy-Chl a. It became apparent that the absorption maxima and the shapes of the curves were identical to those known from the literature (e.g. Refs. 24 and 26). After hydrolysis of the pigments by virtue of the chlorophyllase reaction, giving rise to Chlide a, Chlide b, and 7-hydroxy-Chlide a, respectively, basically the same spectra were obtained (Fig. 3B), which is in agreement with previous findings that the phytol chain in the esterified pigments does not affect their absorption properties as compared with the nonesterified pigments (e.g. Refs. 21 and 32). Upon oxidation with 2,3-dichloro-5,6-dicyanobenzoquinone, striking changes occurred in the absorption properties of all three compounds, however. For Chlide b and Chlide a, spectra were obtained (Fig. 3C) that were indistinguishable from those shown in Fig. 2, B and C, respectively, indicating the production of Pchlide b and Pchlide a. The spectrum of 7-hydroxy-Pchlide a was very similar to that of Pchlide a but was slightly blue-shifted in the red region of the spectrum (Fig. 3C versus Fig. 2A).


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Fig. 3.   Preparation of synthetic 7-hydroxy-Pchlide a, Pchlide b, and Pchlide a. Chemically pure 7-hydroxy-Chl a, Chl a, and Chl b were subjected to the chlorophyllase reaction, and the resulting nonesterified pigments were subsequently dehydrogenated with 2,3-dichloro-5,6-dicyanobenzoquinone. A, absorption spectra in 100% acetone of 7-hydroxy-Chl a, Chl a, and Chl b. B, absorption spectra of 7-hydroxy-Chlide a, Chlide a, and Chlide b. C, absorption spectra of 7-hydroxy-Pchlide a, Pchlide a, and Pchlide b. Note the slight blue shifts in the absorption maximum between the a-type and 7-hydroxy a-type pigments in the red region of the spectrum.

Mass spectrometry was used to further characterize the chemically prepared and natural compounds. Table I shows that the molecular ion peaks at m/z 612.4 ± 0.2, 626.9 ± 0.3, and 628.4 ± 0.2 were indistinguishable for the natural and synthetic pigments. According to previous work, they correspond to 7-hydroxy-Pchlide a (m/z 628.4), Pchlide b (m/z 626.9), and Pchlide a (m/z 612.4) (21, 32). These results thus ultimately confirmed the presence of 7-OH-Pchlide a, Pchlide b, and Pchlide a in etiolated barley leaves.

                              
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Table I
Identification of natural and synthetic pigments by liquid secondary ion mass spectrometry
m/z values were obtained from mass spectra of the molecular ion regions of the various chemically pure compounds as described in Refs. 21 and 32.

Also with isolated, intact barley etioplasts, 7-OH-Pchlide a, Pchlide b, and Pchlide a were readily detectable (Fig. 4A). Careful quantitative pigment measurements showed that Pchlide b (Fig. 4A, peak 2) was ~4-5-fold more abundant in concentration than Pchlide a (Fig. 4A, peak 3). Again, substantial amounts of 7-hydroxy-Pchlide a accumulated (Fig. 4A, peak 1).


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Fig. 4.   In situ conversion of Pchlide b to Pchlide a. Etioplasts were isolated from dark-grown barley plants, lysed, and fractionated into prolamellar bodies and stroma. The prolamellar body fraction in turn was solubilized with n-octyl-beta -D-glucoside. Pigments were extracted with 100% acetone containing 0.1% (v/v) DEP and analyzed by HPLC (see Fig. 1). The different chromatograms show absorbance readings of porphyrin pigments in intact etioplasts (A), lysed etioplasts (B), and solubilized prolamellar bodies (C). Peaks 1-3 correspond to those shown in Fig. 1. The insets show respective quantitative data. Percentages hereby refer to the sum of Pchlide a (gray columns, lanes 1, 3, and 5) and Pchlide b (white columns, lanes 2, 4, and 6) in the different samples, set as 100.

The results presented thus far tempted us to conclude that a major part of Pchlide b originally present in barley etioplasts may be converted to Pchlide a via 7-hydroxy-Pchlide a. We hypothesized that this conversion could be due to 7-formyl reductase activity. To test this idea, conversion of the exogenously administered model substrate ZnPPb was studied in subsequent experiments. Percoll-purified intact etioplasts were allowed to break during the incubation with ZnPPb. Before testing the conversion of the exogenously added ZnPPb, we quantified endogenous pigments extractable with practically pure, nonaqueous acetone (see above) after plastid lysis. HPLC analyses revealed that already during plastid breakage, a massive pigment conversion occurred (Fig. 4B). Both the relative decrease in Pchlide b and 7-hydroxy-Pchlide a levels and the simultaneous increase in the amount of Pchlide a are clearly indicative of such a pigment conversion. This conversion was further pronounced when reisolated prolamellar bodies obtained from lysed etioplasts were solubilized with n-octyl-beta -D-glucoside, a detergent that had frequently been used in previous studies (e.g. Refs. 21 and 34) (Fig. 4C).

Low temperature in situ fluorescence measurements were performed at 77 K (18, 39) in order to analyze the functional state of the different porphyrin pigments. Fig. 5 shows that the intensity of Pchlide F650/657, the predominant fluorescence peak of intact prolamellar bodies of etioplasts (see Introduction), was drastically reduced upon etioplast lysis and subsequent membrane solubilization (Fig. 5, dashed and dotted lines, respectively, versus solid line). With n-octyl-beta -D-glucoside-solubilized membranes, in fact, no Pchlide F650/657 could be traced. Instead, Pchlide F628/632 became the prevalent spectral pigment species (Fig. 5, dotted line).


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Fig. 5.   Low temperature (77 K) fluorescence emission analysis at an excitation wavelength of 440 nm of porphyrins present in intact etioplasts (Intact), lysed etioplasts (Lysed), and isolated prolamellar bodies after their solubilization with n-octyl-beta -D-glucoside (Solubilized).

We next analyzed the effect of externally added ZnPPb. We assumed that the pigment, if applied in excess, should be able to compete out Pchlide b to Pchlide a conversion. Percoll-purified intact etioplasts were supplemented with ZnPPb and then lysed hypotonically, and the membranes were sedimented and solubilized with n-octyl-beta -D-glucoside. Binding of ZnPPb, as well as that of ZnPPa used as a control, to the solubilized membranes was tested in two different ways. We first performed low temperature spectroscopic measurements at 77 K. These showed that almost indistinguishable levels of ZnPPa and ZnPPb were bound to the membranes (Fig. 6). Interestingly, in neither case was a long wavelength pigment species restored which emitted at 657 nm (Pchlide F650/657) (Fig. 6).


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Fig. 6.   Low temperature (77 K) fluorescence emission analysis at 440 nm to quantify binding of externally added ZnPPa or ZnPPb to isolated prolamellar bodies solubilized with n-octyl-beta -D-glucoside. Details are given under "Results."

As a second method, we quantified ZnPPa and ZnPPb binding by HPLC (26). However, also by these pigment measurements, no difference in ZnPPa and ZnPPb binding could be seen (Fig. 7, Binding, compare columns 1 and 2).


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Fig. 7.   Porphyrin binding and conversion characteristics of solubilized prolamellar bodies. For testing porphyrin binding, either ZnPPa or ZnPPb was used (columns 1 and 2, respectively). For conversion, only ZnPPb was added as described under "Results" (columns 3-10). As indicated, the assays were supplemented with or without stromal extract (St.) and a mixture of NADPH, glucose 6-phosphate, glucose-6-phosphate-dehydrogenase, ferredoxin-NADPH oxidoreductase plus ferredoxin (Su.). Conversion of ZnPPb to ZnPPa during a 60-min dark incubation was followed by HPLC, using aliquots taken out at 10-min intervals. The data refer to time point zero, which is identical to that shown in column 1.

Conversion of bound pigments was tested in a subsequent experiment. Detergent-treated membranes were subjected to a prolonged dark incubation, and pigment conversion was analyzed by fluorescence and HPLC measurements. Again, no change could be seen (Fig. 7, Conversion, columns 7 and 8 versus columns 3-6), demonstrating that either pigment was stable and not converted into other compounds. The addition of stromal extract to the ZnPPb-containing assays (Fig. 7, Conversion, columns 7 and 8 versus columns 3-6) and/or NADPH, glucose 6-phosphate, glucose-6-phosphate-dehydrogenase, ferredoxin-NADPH oxidoreductase plus ferredoxin, which had collectively been used to restore Chl(ide) b reductase activity in previous studies (23, 26), proved unsuccessful in our experiments (Fig. 7, columns 9 and 10).

The finding that the n-octyl-beta -D-glucoside-solubilized membranes bound approximately the same levels of ZnPPb and ZnPPa (Figs. 5 and 6) at first glance seemed to contradict the LHPP model (18). According to this model, at least a 50-fold difference in ZnPPb binding and a 2-fold difference in ZnPPa binding should have been seen, reflecting the 5-fold higher abundance of the PORA as compared with that of the PORB and their ~10-fold different substrate specificities (18). Whereas PORA expressed in vitro has been shown to bind 10-fold higher levels of ZnPPb as compared with ZnPPa, PORB displayed a 10-fold greater specificity for ZnPPa and bound only little ZnPPb (18, 31).

An explanation for this apparent paradox could be that the PORA was denatured, was partially degraded, or had become soluble upon etioplast lysis and/or membrane solubilization, including respective centrifugation steps. To follow the fate of the PORA and PORB in the different fractions, we consequently performed Western blot analyses. For comparison, we preflashed isolated, intact etioplasts before analysis and subfractionation with a saturating 1-ms flash of white light, which had previously been used to induce the disintegration of the prolamellar body (13).

Fig. 8A shows that with the dark-incubated, nonflashed samples, both the PORA and PORB proteins were detectable in the intact etioplasts and in the respective sediment fraction obtained after plastid lysis and centrifugation. Upon solubilization of the sedimented membranes, the picture then changed. Only the PORB was retained in the sediment fraction, whereas the PORA was almost quantitatively released into the respective supernatant (Fig. 8A). With the preflashed sample, this release was already detectable upon plastid lysis. Then almost all PORA was found in the supernatant fraction and only traces remained bound to the resedimented membranes (Fig. 8A). Upon detergent treatment, this remainder was released into the supernatant obtained after centrifugation of the assays.


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Fig. 8.   Protochlorophyllide b to Pchlide a conversion in relation to the light-induced disintegration of the prolamellar body. Etioplasts were prepared from dark-grown barley plants and either kept in complete darkness (Darkness, lanes 1-5) or exposed to a saturating 1-ms flash of white light (Flashed, lanes 6-10). Protein and pigments were then extracted from the plastids or respective subfractions as described in Fig. 4. A, high resolution Western blot analysis of POR-related proteins. Protein was resolved electrophoretically and blotted onto a nitrocellulose membrane, and POR-related proteins were detected with a POR-specific antiserum. The blots show the PORA (36 kDa) and PORB (38 kDa) in the flashed and nonflashed etioplasts before lysis (Intact, lanes 1 and 6), after lysis (Lysed, lanes 2 and 3 versus lanes 7 and 8), and in solubilized etioplast inner membranes (Solubilized, lanes 4 and 5 versus lanes 9 and 10). Both the respective membrane pellet (P, lanes 2, 4, 7, and 9) and supernatant fractions (S, lanes 3, 5, 8, and 10) were analyzed. In all cases, protein equivalent to 25 µg of bovine serum albumin was probed per lane. B, HPLC chromatogram of pigments found in lysed, flashed etioplasts. Intact etioplasts were prepared as described before and flashed. Pigments were extracted from the total sample, corresponding to lanes 7 plus 8 in A, with 100% acetone containing 0.1% (v/v) DEP and analyzed on a C18 column. Peaks 1-3 correspond to those shown in Fig. 1. Peak 4 is due to Chlide a, as shown in an accompanying paper (31).

The various findings reported thus far implied that Pchlide b to Pchlide a conversion may be related to the release of the PORA from the prolamellar body, either artificially as a result of detergent solubilization of the isolated membranes or, more naturally, as part of the light-induced disintegration of these structures. Because the latter process should allow PORA, which is per se a Pchlide-reducing enzyme (10), to regain its activity, we quantified the level of Chlide b and Chlide a as well as Pchlide b and Pchlide a in the various fractions highlighted in Fig. 8A.

Fig. 8B shows a representative HPLC chromatogram. Table II summarizes the results. They confirmed that 7-formyl reductase is present both in the flashed and nonflashed etioplast samples. In the latter, it was rapidly activated upon plastid lysis and membrane solubilization and converted practically all of the preexisting Pchlide b to Pchlide a. In the preflashed sample, 7-formyl reductase was active as well, but it did not seem to gain its full activity. In the supernatant of preflashed, lysed plastids, only one-third of the total pigment was accounted for by Pchlide a. The remainder was present as Pchlide b. In the respective pellet fraction, we recovered ~3-fold lower levels of Pchlide b relative to Pchlide a and found significant levels of Chlide a (Table II).

                              
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Table II
Relative pigment levels in intact barley etioplasts and different plastid subfractions
Pigments were extracted from the various plastid fractions given in Fig. 8 with 100% acetone containing 0.1% (v/v) DEP and separated by HPLC (see "Materials and Methods"). Quantification of HPLC-separated porphyrins was achieved by absorbance measurements at 455 nm, using chemically synthesized Pchlides a and b as standards. Chlorins were quantified as described in Refs. 39 and 41. Numbers refer to the total pigment levels in each fraction, set as 100. ND, nondetectable pigment levels.

Upon membrane solubilization, only Pchlide a could be detected in the resedimented membranes of the preflashed etioplasts (Table II). Remarkably, this fraction did not contain either Chlide b or Chlide a (Table II); nor were we able to trace any Pchlide b. In the respective supernatant, the only detectable pigment was Chlide a (Table II). This suggested that residual Pchlide b present in the pellet of the lysed etioplasts had been converted to Pchlide a.

    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

In the present study, we readdressed the previously published plastid work-up and pigment extraction procedure of Scheumann et al. (21), which involves hypotonic plastid lysis, the sedimentation of the prolamellar body, and subsequent membrane solubilization. Finally, the solubilized membranes were sedimented, and the obtained pellet and supernatant fractions, respectively, were characterized further.

Our results demonstrate that plastid lysis and membrane solubilization collectively lead to the denaturation of the prolamellar body, the release of the PORA, and the conversion of most, if not all, of the total Pchlide b to Pchlide a. The latter reaction was presumably catalyzed by 7-formyl reductase. This enzyme had originally been implicated in the Chl b to Chl a reaction cycle of chloroplasts during photosynthetic acclimation and leaf senescence (26). But it is also highly active in etioplasts, as shown in this and previous studies (22-26). We assume that 7-formyl reductase may be involved in fine tuning the amounts of Pchlide b and Pchlide a and thereby could regulate the steady-state level of LHPP in etioplasts.

Flash light illumination of intact etioplasts, which has for a long time been known to induce the disintegration of the prolamellar body (13), caused the simultaneous release of the PORA and Pchlide b from the inner plastid membranes. But it did not induce the immediate enzymatic reduction of Pchlide b to Chlide b or the quantitative transformation of Pchlide b to Pchlide a. These important results lend more, although indirect, support to our previous conclusion that the PORA remains, in the first place, catalytically inactive as a Pchlide b-reducing enzyme (18). Moreover, they demonstrate that 7-formyl reductase activity is partially suppressed during the light-induced transformation of etioplasts into chloroplasts. Although we do not yet know the reasons for this effect, we hypothesize that 7-formyl reductase may be involved in controlling the rate of PORA-driven Chlide b synthesis in illuminated plants. It is tempting to speculate that PORA-derived Chlide b could play regulatory roles for the establishment of the light-harvesting structures in etiolated plants at the beginning of illumination, whereas Chl(ide) b synthesized by virtue of Chlide a oxygenase (29, 30, 41) in light-adapted plants could serve housekeeping functions during photosynthesis (for reviews, see Refs. 42 and 43). According to recent work (44), Chlide a oxygenase is well able to accept both Pchlide a and Chlide a as substrate, although with different apparent affinities, but its expression in etiolated, illuminated, and light-adapted plants and its localization have not yet been examined.

In addition to these aspects, the results presented in this study answer the long lasting question of whether or not Pchlide b is occurring in etiolated plants (18-21, 45). Our findings show that Pchlide b is present in barley etioplasts and indeed accounts to amounts well compatible with the LHPP model (18, 19). According to this model, Pchlide b was supposed to be ~4-5-fold more abundant than Pchlide a. However, given that Pchlide b is metabolically unstable, the pigment rather easily escapes the detection. This could explain why previous pigment extraction procedures failed to detect the pigment (21). Further work is needed to see whether there is a Pchlide b/Pchlide a interconversion cycle similar to that reported previously for Chl a and Chl b (29).

    ACKNOWLEDGEMENTS

This work was performed in part in the Department of Prof. Dr. E. W. Weiler at the Institute for Plant Physiology, Ruhr-Universität Bochum, Bochum, Germany. We are grateful to Prof. Weiler for stimulating interest and continuous support of the work. We thank Dr. M. Kuntz (Centre National de la Recherche Scientifique (CNRS), Grenoble, France) for critical reading of the manuscript and help with the HPLC.

    FOOTNOTES

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| To whom correspondence should be addressed: Lehrstuhl für Pflanzenphysiologie, Universität Bayreuth, Universitätsstr. 30, 95447 Bayreuth, Germany. Tel.: 49-921-55-26-27; Fax: 49-921-75-77-442; E-mail: christiane.reinbothe@uni-bayreuth.de.

Published, JBC Papers in Press, October 24, 2002, DOI 10.1074/jbc.M209737200

    ABBREVIATIONS

The abbreviations used are: Pchlide, protochlorophyllide; Chlide, chlorophyllide; Chl, chlorophyll; DEP, diethyl pyrocarbonate; HPLC, high performance liquid chromatography; LHPP, light-harvesting POR-Pchlide complex; POR, NADPH:protochlorophyllide oxidoreductase; ZnPP, zinc protopheophorbide..

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
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