Atypical Rho GTPases Have Roles in Mitochondrial Homeostasis and Apoptosis*

Åsa Fransson, Aino Ruusala, and Pontus AspenströmDagger

From the Ludwig Institute for Cancer Research, Biomedical Center, Box 595, S-751 24 Uppsala, Sweden

Received for publication, August 22, 2002, and in revised form, November 22, 2002

    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The human genomic sequencing effort has revealed the presence of a large number of Rho GTPases encoded by the human genome. Here we report the characterization of a new family of Rho GTPases with atypical features. These proteins, which were called Miro-1 and Miro-2 (for mitochondrial Rho), have tandem GTP-binding domains separated by a linker region containing putative calcium-binding EF hand motifs. Genes encoding Miro-like proteins were found in several eukaryotic organisms from Saccharomyces cerevisiae, Caenorhabditis elegans, and Drosophila melanogaster to mammals, indicating that these genes evolved early during evolution. Immunolocalization experiments, in which transfected NIH3T3 and COS 7 cells were stained for ectopically expressed Miro as well as for the endogenous Miro-1 protein, showed that Miro was present in mitochondria. Interestingly, overexpression of a constitutively active mutant of Miro-1 (Miro-1/Val-13) induced an aggregation of the mitochondrial network and resulted in an increased apoptotic rate of the cells expressing activated Miro-1. These data indicate a novel role for Rho-like GTPases in mitochondrial homeostasis and apoptosis.

    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Small GTPases have been shown to be pivotal signaling intermediates in cell growth, cell cycle progression, cell survival, cell transformation, and cell trafficking (1-3). The potential of small GTPases to function as signaling switches resides in their ability to cycle between active, GTP-bound states, and inactive, GDP-bound states. This cycling is regulated in a precise manner by guanine nucleotide exchange factors (4), GTPase-activating proteins, and guanine nucleotide dissociation inhibitors (GDIs)1 (5, 6). Guanine nucleotide exchange factors stimulate the replacement of GDP by GTP, whereas GTPase-activating proteins stimulate the intrinsic GTP hydrolysis of the GTPase. GDIs act by blocking GDP dissociation, and in resting cells, Rho GTPases are thought to reside in an inactive complex with RhoGDI. According to this model, cell stimulation leads to a dissociation of the complex, resulting in the subsequent activation of the released Rho GTPase (6).

The proto-oncogene ras was the first small GTPase to be identified over 20 years ago (see Ref. 7 and references therein); however, the recently published draft of the human genome indicate the presence of roughly 150 genes encoding small GTPases (8, 9). Among these genes, the rho subfamily has been shown to be involved specifically in regulating the morphogenic and motile properties of vertebrate cells, primarily by affecting the actin filament system (1-3). Most of our current knowledge regarding the Rho GTPases originates from work on the triad RhoA, Rac1, and Cdc42, which have each been found to regulate distinct actin-containing structures. RhoA regulates the formation of focal adhesions and the subsequent assembly of stress fibers (10); Rac1 regulates the formation of membrane lamellae or lamellipodia (11), and Cdc42 triggers the outgrowth of peripheral spike-like protrusions, also known as filopodia (12). However, over the years, it has become evident that the Rho GTPases are involved in the regulation of several additional cellular processes. Rac1 and Cdc42 participate in transcriptional control via the Jun N-terminal kinase/stress-activated protein kinase and p38MAPK signaling cascades; RhoA has a role in serum-response factor-regulated gene transcription, and all three contribute to transcriptional activation via NFkappa B signaling pathway (1-3). Furthermore, the Rho GTPases are also participants in signaling pathways that control cell cycle progression and apoptosis (13).

So far, 20 distinct genes encoding family members of Rho GTPases have been identified. These genes can be further divided into seven subgroups: cdc42 (consisting of cdc42, tc10, tcl, chp, and wrch1/chp2), rac (rac1, rac2, rac3, and rhoG), rho (rhoA, rhoB, and rhoC), rnd (rnd1, rnd2, and rnd3), rhoD (rhoD and rif), and rhoH (see Ref. 14 and references therein and Refs. 15-17). The seventh subgroup is formed by three additional rho-like genes, called kiaa0740, kiaa0717, and kiaa0878, which have been identified by the Kazusa Institute in Japan (18). These genes encode human orthologs of the Drosophila melanogaster rhoBTB gene, suggesting that more appropriate names for them would be rhoBTB1, rhoBTB2, and rhoBTB3, respectively (18, 19).

The work in this article describes a novel subgroup of the Rho GTPases. This subgroup is formed by two Rho-like genes, which were named Miro (for mitochondrial Rho). These genes encode proteins that are similar to the Rho GTPases in their N-terminal GTPase domains, but in addition they contain potential calcium-binding motifs, so called EF-hands (20, 21), and an additional putative GTPase domain in the C terminus which is diverged from the Rho GTPases. Data presented in this report showed that ectopically expressed Miro-1 and Miro-2, as well as endogenous Miro-1, are present at mitochondria. Furthermore, transient transfection of a constitutively active Miro-1 (Miro-1/Val-13) destroyed the mitochondrial network, which collapsed into perinuclear assemblies; moreover, it resulted in an increased apoptosis rate. These data suggested a role for Miro proteins in mitochondrial homeostasis and in apoptosis.

    EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

DNA Work-- Miro-1 and Miro-2 were identified by searching the public DNA and protein data bases for novel members of the Rho GTPases employing the Blast search motor. The translated amino acid sequence for Miro-1 and Miro-2 were then used to search EST data bases for the presence of EST clones encoding putative full-length Miro-1 and Miro-2, and two such clones were obtained from the UK Human Genome Mapping Project Resource Centre in Hinxton, Cambridge, UK. The nucleotide sequences of Miro-1 and -2 have been deposited in the GenBankTM data base with accession numbers AJ517412 (Miro-1) and AJ517413 (Miro-2). DNA fragments encoding full-length Miro-1 and Miro-2 were generated by PCR and subcloned into the pRK5Myc vector. The Miro-1/Val-13 and Miro-1/Asn-18 mutants were generated employing the QuickChange protocol (Stratagene) according to the procedure provided by the manufacturer. The fidelity of all DNA constructs was conformed by DNA sequencing employing the ABI Prism 310 Genetic Analyzer.

Northern Blot Analysis-- cDNA probes of the open reading frame of Miro-1 and Miro-2, respectively, were labeled with [32P]dCTP employing the rediprime labeling kit (Amersham Biosciences). The probes were thereafter hybridized to hybridization-ready Northern blots (Human Multiple Tissue Northern blot, Clontech) according to the ExpressHyb (Clontech) protocol provided by the manufacturer.

Cell Cultivation and Cell Transformation-- NIH3T3 cells and COS 7 cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% FCS and penicillin/streptomycin at 37 °C in an atmosphere of 5% CO2. For immunostaining purposes, cells were seeded on coverslips and transfected by LipofectAMINE or LipofectAMINE Plus (Invitrogen) according to the protocols provided by the manufacturer. Transfections of COS 7 cells for immunoprecipitation purposes were performed by the DEAE-dextran method essentially as described before (22). Metabolic 35S labeling of Miro-1 was performed as described before (22). Miro-1 immunoprecipitates were subjected to SDS-PAGE, and the gels were fixed for 15 min in 25% methanol and 7.5% acetic acid. The gels were thereafter dried and exposed on a PhosphorImager (Fujix BAS 2000).

Antibodies and Immunocytochemistry-- A Miro-1-specific antibody was produced by immunizing rabbits with a keyhole limpet hemocyanin-conjugated Miro-1-specific peptide representing amino acid residues 560-574 of human Miro-1. Mouse monoclonal anti-Myc (9E10) and rabbit polyclonal anti-Myc antibodies (Santa Cruz Biotechnology) were used to determine the subcellular localization of Myc-tagged Miro-1 and Miro-2 as well as Miro-1 mutants. The mouse monoclonal M30 antibody (Roche Molecular Biochemicals) was used to stain apoptotic cells. MitoTracker Green FM (Molecular Probes) and a mouse monoclonal anti-cytochrome c antibody (Pharmingen) were used according to the protocol supplied by the manufacturer to visualize mitochondria, and 4',6-diamidino-2-phenyindole (DAPI) was used to visualize nuclei. Filamentous actin was visualized by TRITC-conjugated phalloidin (Sigma). Microtubules were visualized by a mouse monoclonal anti-alpha -tubulin antibody (Sigma). The following secondary antibodies were used: fluorescein isothiocyanate (FITC)-conjugated anti-mouse, FITC-conjugated anti-rabbit, TRITC-conjugated anti-rabbit (Dako), and a TRITC-conjugated anti-mouse (Jackson ImmunoResearch). The caspase inhibitors Z-VAD-FMK and Z-DEVD-FMK were obtained from Calbiochem. For the immunocytochemistry, transiently transfected NIH3T3 and COS 7 cells were grown on coverslips and fixed in 3% paraformaldehyde in PBS for 20 min at 37 °C. The cells were washed with PBS and permeabilized in 0.2% Triton X-100 in PBS for 5 min. The cells were thereafter washed again and incubated in the presence of 5% FCS in PBS for 30 min. Primary as well as secondary antibodies were diluted in PBS containing 5% FCS. Cells were incubated with primary antibodies followed by secondary antibodies for intervals of 1 h with a washing step in between. The coverslips were mounted in Fluoromount-G (Southern Biotechnology Associates) on object slides. Cells were photographed by a Hamamatsu ORCA CCD digital camera employing the QED Imaging System software using a Zeiss Axioplan2 microscope.

    RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Identification of Miro Genes-- Searches in the public genomic DNA sequence data bases generated by the human genome projects implicated the presence of several novel members of the Rho GTPases (8, 9). Expressed sequence tag (EST) clones, encoding two putative Rho GTPases, were obtained. Sequence analysis demonstrated that these clones encoded two related Rho GTPases, which were named Miro-1 and Miro-2, respectively. Miro-1 and Miro-2 were found to be 60% identical to each other, and both proteins encoded polypeptides of 618 amino acid residues with predicted molecular masses of 70 kDa (Fig. 1, A and B). The amino acid sequence of Miro-1 and -2 revealed an unusual domain organization; the N-terminal part encoded a GTPase domain, which is related to the Rho GTPases (Fig. 1C). This domain was followed by two EF hands (Fig. 1D), a type of domain that confers binding to calcium ions (20, 21). Surprisingly, a second potential GTP-binding domain, but in this case without homology to the Rho GTPases, was found in the C terminus (Fig. 1E). In addition, Miro lacks a so-called CAAX motif, a domain usually found in the C termini of small GTPases. The CAAX motif undergoes post-translational isoprenylation, a modification that confers membrane targeting of the protein. The unusual domain organization of Miro implicates that the proteins are regulated in a manner distinct from the archetypal Rho GTPases.



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Fig. 1.   Primary structure of Miro. A, domain organization of human Miro-1 and Miro-2. B, alignments of the amino acid sequences of human Miro-1 and Miro-2. Dark gray shading indicates identical amino acid residues in Miro-1 and Miro-2, and light gray shading indicates conserved amino acid substitutions. Alignments were performed aided by the DNAssist software. C, comparison of GTPase domain I of Miro-1 and Miro-2 with human Rac1, RhoA, and Cdc42. * marks the conserved amino acid residues glycine 12 and glutamine 61 of Rho GTPases. Amino acid substitutions in any of these positions most often result in defect GTP hydrolysis activity of the GTPase. Miro-1 has proline and alanine residues in these positions, whereas Miro-2 has alanine residues in both positions. D, comparison of the EF hands of Miro proteins. X-Y-ZG#Ix---z marks the consensus calcium-binding motif typical for EF hands (20, 21). E, comparison of the GTPase domain II of Miro proteins from different eukaryotic organisms. F, dendritic tree representation of the similarity of all currently known Miro proteins. G, Northern blot analysis of human Miro-1 and Miro-2 expression in the human tissues depicted in the panel.

Interestingly, data base searches demonstrated the presence of genes, orthologous to Miro, in several eukaryotic organisms including Saccharomyces cerevisiae, Schizosaccharomyces pombe, Caenorhabditis elegans, Arabidopsis thaliana, and D. melanogaster (Fig. 1F). This observation indicated that the Miro genes evolved early during evolution. Northern blot analysis, employing human multiple tissue Northern blots, demonstrated the presence of Miro mRNA in most human tissue (Fig. 1G). Miro-1 mRNA of a size of 3 kb was abundant in heart and skeletal muscle, and Miro-2 mRNA of 2.4 kb was abundant in heart, liver, skeletal muscle, kidney, and pancreas (Fig. 1G).

Miro Are Localized to Mitochondria-- The Rho GTPases have been shown to have a major impact on the organization of the actin cytoskeleton (1-3). For this reason the effect on the cytoskeletal organization by ectopic expression of Miro-1 and -2 was studied. Plasmids encoding Myc-tagged Miro-1 and Miro-2 were transiently transfected into NIH3T3 cells after which Miro expression was visualized by a Myc-specific antibody, whereas filamentous actin was visualized with TRITC-conjugated phalloidin. Microtubules were visualized by an antibody against alpha -tubulin. Neither Miro-1 nor Miro-2 expression visibly affected the organization of the actin filament system (Fig. 2, A and C) or the microtubules (Fig. 2, B and D). Interestingly, it was noticed that Miro staining followed a very distinct pattern; both Miro-1 and Miro-2 were organized in worm-like structures in a manner reminiscent of the mitochondrial network (Fig. 2). A Miro construct expressing only the N-terminal GTPase domain did not localize into such worm-like structures, instead this deletion mutant was dispersed evenly in the cytoplasm of transfected cells (Fig. 2E). In order to test if the localization of Miro coincided with mitochondria, NIH3T3 cells were transiently transfected with Myc-Miro-1 and co-stained with the mitochondria marker MitoTracker Green FM. Miro-1 overlapped to a large extent with the MitoTracker staining, suggesting that Miro-1 is present at the mitochondria (Fig. 2F, detail). Transiently transfected Miro-2 colocalized with mitochondrial marker in a similar fashion (data not shown). In addition, Myc-Miro-1 staining colocalized with the mitochondria protein cytochrome c, further emphasizing the notion of Miro as a mitochondria-associated protein (Fig. 2G).


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Fig. 2.   Ectopically expressed Miro is present in mitochondria. A and B, subcellular localization of Myc-tagged Miro-1 was detected by a Myc-specific mouse antibody followed by a FITC-conjugated anti-mouse antibody. Filamentous actin was detected by TRITC-conjugated phalloidin (A). Microtubules were detected by a mouse anti-alpha -tubulin followed by a TRITC-conjugated anti-mouse antibody (B). C and D, Myc-tagged Miro-2, filamentous actin and microtubules were detected as described in A and B. E, subcellular localization of Myc-Miro-1/N-terminal GTPase domain was detected by a Myc-specific rabbit antibody followed by a FITC-conjugated anti-rabbit antibody; microtubules were detected by a mouse anti-alpha -tubulin antibody followed by a TRITC-conjugated anti-mouse antibody, whereas filamentous actin was detected by Alexa350-conjugated phalloidin. F, subcellular localization of Miro-1 was detected by a Myc-specific mouse antibody followed by a TRITC-conjugated anti-mouse antibody. Mitochondria were detected by the MitoTracker Green FM fluorescent probe. G, subcellular localization of Myc-Miro-1 was detected by a Myc-specific rabbit antibody followed by a TRITC-conjugated anti-rabbit antibody. Cytochrome c was detected by a mouse anti-cytochrome c antibody followed by a FITC-conjugated anti-mouse antibody. Bar represents 20 µm.

A peptide derived from the C terminus of Miro-1 was synthesized and used to raise a Miro-1-specific antiserum in rabbits. The antiserum was affinity-purified over a column of Sulfolink-conjugated Miro-1-specific peptide. The antiserum, as well as the affinity-purified antibody, was tested in immunoprecipitation experiments employing lysates from 35S-labeled COS 7 cells transiently transfected with Myc-Miro-1. The Miro-1 antiserum and the affinity-purified antibody, as well as the Myc antibody, precipitated a band of 70 kDa. This band was neither precipitated by pre-immune serum nor with the Miro-1 antiserum pre-blocked with the antigenic Miro-1-specific peptide (Fig. 3A). Next, a number of cell lines were tested for expression of Miro-1 by Western blotting. Human hepatocellular carcinoma Hep G2 cells and human embryonic kidney HEK293T cells contained high levels of Miro-1, whereas NIH3T3 fibroblasts and COS 7 cells expressed lower levels of Miro-1 (Fig. 3B). Hep G2 and HEK293T cells grow in a fashion that made visualization of their mitochondria difficult. For this reason, despite the lower expression of Miro-1, COS 7 cells turned out to be more useful for immunocytochemical analysis of the mitochondrial network. The Miro-1-specific antibody was used to stain COS 7 cells in order to examine if endogenous protein was localized to mitochondria. The staining pattern overlapped to a large extent with cytochrome c; moreover, the Miro-1-specific staining was quenched by pre-treatment with the blocking peptide. Collectively, these observations strongly suggested that endogenous Miro-1 is indeed a mitochondrial protein (Fig. 3C).


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Fig. 3.   Endogenous Miro is present in mitochondria. A, Myc-tagged Miro-1 was immunoprecipitated from 35S-labeled COS 7 cells with 5 µl of Myc-specific antibody, 10 µl of pre-immune serum, 10 µl of Miro-1-specific antiserum, or 2 µg of Miro-1-specific purified antibody. B, Western blot (WB) with a Miro-1-specific antibody to detect Miro-1 proteins in the lysate denoted in the figure label. C, subcellular localization of endogenous Miro-1 was detected by a Miro-1-specific rabbit antibody followed by a TRITC-conjugated anti-rabbit antibody. Cytochrome c was detected by a mouse anti-cytochrome c antibody followed by a FITC-conjugated anti-mouse antibody. In the experiment in the lower panel, the Miro-1-specific antibody was preincubated with 200 µM of the blocking peptide. Bar represents 20 µm.

Activation of Miro-1 Induces Collapse of the Mitochondrial Network-- Studies employing constitutive active and dominant negative mutants of Rho GTPases have been extensively used to examine the involvement of these proteins in numerous signal transduction pathways (1, 3). In analogy with the mutation employed for studies of RhoA, Rac1, and Cdc42, we made a constitutive active mutant Miro-1 in which the amino acid residue at position 13 was replaced with a valine. This mutation locks the GTPase in a GTP-bound conformation. By the same analogy, a dominant negative Miro-1 was made by mutating codon 18 to an asparagine, a mutation that locks the GTPase in a GDP-bound conformation. Myc-Miro-1/Asn-18 or Myc-Miro-1/Val-13 was transiently transfected into NIH3T3 cells or into COS 7 cells. The overexpression of Miro mutants had no obvious effects on the cell morphology or on the organization of the actin filament system or the microtubules (Fig. 4A). Interestingly, the Miro-1/Val-13 staining was altered in a striking manner compared with wt-Miro-1. The Miro-1/Val-13-expressing cells had lost the mitochondrial network, and instead Miro-1/Val-13 was localized into large perinuclear assemblies in both NIH3T3 (data not shown) and COS 7 cells (Fig. 4B). Co-staining with MitoTracker Green FM indicated that the mitochondrial network had in fact collapsed in these cells, and instead the mitochondria were organized in these perinuclear assemblies (Fig. 4B). Myc-Miro-1/Asn-18 expressing cells appeared less affected, and a majority of the cells exhibited a normal mitochondrial network; however, some cells with condensed mitochondria were seen also under these conditions (Fig. 4B, arrowhead). These data suggested that the GTP/GDP loaded status of Miro-1 was important for the heterogeneity of the mitochondrial network and hence the mitochondrial homeostasis.


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Fig. 4.   Ectopic expression of mutant Miro-1 induce an aggregation of the mitochondrial network. A, subcellular localization of Miro-1, Miro-1/Val-13, and Miro-1/Asn-18 was detected by a Myc-specific rabbit antibody followed by either TRITC-conjugated phalloidin to visualize F-actin or a mouse anti-alpha -tubulin antibody followed by a TRITC-conjugated anti-mouse antibody to visualize microtubules. DAPI was used to stain cell nuclei. B, Myc-tagged Miro-1, Miro-1/Val-13, and Miro-1/Asn-18 was detected by a Myc-specific mouse antibody followed by a TRITC-conjugated anti-mouse antibody. Mitochondria were detected by the MitoTracker Green FM. C, Myc-tagged Miro-1, Miro-1/Val-13, and Miro-1/Asn-18 was detected by a Myc-specific rabbit antibody followed by a TRITC-conjugated anti-rabbit antibody. Cytochrome c was detected by a mouse anti-cytochrome c antibody followed by a FITC-conjugated anti-mouse antibody. Bar represents 20 µm.

We next studied if cytochrome c was still present in the collapsed mitochondrial membranes. Cytochrome c has been shown to be released from the mitochondria during apoptosis (23); therefore, COS 7 cells were transiently transfected with Myc-Miro-1 or Myc-Miro-1/Val-13 and analyzed for Myc-Miro-1 expression and cytochrome c localization 24 and 48 h post-transfection. In Myc-Miro-1-expressing COS 7 cells the cytochrome c colocalized exactly with the Myc-Miro-1 (Fig. 4C) similarly to the transfected NIH3T3 fibroblasts (Fig. 2G). This was further emphasized by confocal microscopy analysis, which demonstrated a colocalization between cytochrome c and Miro-1 (data not shown). Myc-Miro-1/Val-13 overexpression resulted again in a loss of the mitochondrial network. Miro-1/Val-13 was present in the mitochondrial assemblies, but it was also dispersed into the cytoplasm (Fig. 4C). The cytochrome c staining was present in the mitochondrial assemblies, but an increased number of cells with cytoplasmic cytochrome c staining were seen after 48 h (Fig. 4C, arrowhead).

Ectopic Expression of Miro-1 Induces Apoptosis-- Disturbances of mitochondrial homeostasis have been shown to result in an increased rate of apoptosis (24). We noticed a clear reduction of Miro-1/Val-13-expressing cells over time, compared with the amount of wt-Miro-1-expressing cells, which could implicate an increased rate of apoptosis in this cell population. For this reason we stained COS 7 cells transiently transfected with Myc-Miro-1, Myc-Miro-1/Val-13, or Myc-Miro-1/Asn-18 with a Myc-specific antibody as well as with the apoptosis marker antibody M30 (which specifically recognizes the caspase-cleaved cytokeratin 18). We calculated the amount of M30 positive cells that was also transfected with the Miro-1 mutants. A clear increase in M30 positive cells was noticed in the population of cells expressing Miro-1/Val-13. After 24 h the M30 positive and Myc-Miro-1/Val-13-expressing cells often showed a network of cytokeratin filaments (Fig. 5A). However, after a longer time the M30 staining was confined to dot-like structures, in particular in the frequent rounded up Myc-Miro-1/Val-13-expressing cells (Fig. 5B). The Miro-1/Val-13 and M30 positive cells also had fragmented nuclei, which could be visualized by DAPI staining (Fig. 5C). We counted the ratio between M30 positive cells and Myc-Miro-1-expressing cells. In the population of Miro-1/Val-13 33.0% of the cells were apoptotic. In contrast in cells expressing Miro-1 wild type or Asn-18 only 10.7 or 9.2%, respectively, of the cells were undergoing apoptosis (Fig. 5D). The Miro-1/Val-13 induced apoptosis was drastically reduced from 33 to 7.5% when the cells were treated with the caspase inhibitor Z-VAD-FMK (Fig. 5D). Treatment of the cells with caspase-3 inhibitor Z-DEVD-FMK also reduced the Miro-1/Val-13-induced apoptosis, from 33 to 14.3% (Fig. 5D), indicating that this response is dependent on the caspase cascade. Collectively, these data implicate that Miro proteins are mitochondrial constituents and that the activity of Miro affects the homeostasis of mitochondria leading to an increased rate of apoptosis.


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Fig. 5.   Ectopic expression of the mutant Miro-1/Val-13 induced an increased apoptotic rate. A-C, apoptotic cells were detected by the M30 antibody, which specifically recognizes the caspase-cleaved cytokeratin 18, followed by FITC (TRITC in C)-conjugated anti-mouse antibody, and by DAPI staining (C) to visualize degraded nuclei in the apoptotic cells. Miro-1 was detected by a Myc-specific rabbit antibody followed by a TRITC (FITC in C)-conjugated anti rabbit antibody. Bar represents 20 µm. D, the ratio of Miro-1-expressing cells and M30 positive, apoptotic cells, was determined by counting cells under the microscope. For each experiment, at least 200 cells were scored using the 63× immersion oil objective, and the values shown in the diagram represent the means from at least 3 independent transfection experiments. Miro-1/Val-13-transfected cells were treated with 100 µM Z-VAD-FMK or 100 µM Z-DEVD-FMK, which was added 24 h post-transfection. After addition of the inhibitors the cells were incubated for an additional 24 h and then scored for apoptosis.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The roles of Cdc42, Rac, or Rho in cell migration and morphogenesis are well documented. A majority of the studies involving Rho GTPases are still focused to this archetypal triad; however, there are increased indications that other Rho GTPases have equally important cellular roles. For example, members of the Rnd subfamily have been suggested to function as antagonists to Rho but, in contrast to Rho, their activation leads to stress fibers breakdown and a decreased attachment to the substratum, thereby causing a rounding up of cells (25). RhoH has been implicated to counteract Rac-dependent activation of the p38MAPK and NFkappa B signaling pathways (26). Furthermore, RhoD and Rif appear to have specific roles during membrane trafficking, but they also induce the formation of filopodia, apparently by a mechanism distinct from Cdc42 or the Cdc42-like GTPases (16, 27). Finally, Chp and Wrch1 are two closely related Cdc42 family GTPases that are involved in the formation of peripheral protrusions such as lamellipodia and filopodia (17, 28). Collectively, these observations form the basis for a model, which suggests that dynamic reorganizations of cellular processes require a tightly balanced regulated activation of different Rho GTPases.

Miro proteins are unique not only by means of their size, because they are bigger than the classical small GTPases, but also by the presence of additional protein domain structures. Another group of atypical Rho-like proteins, the RhoBTBs, has also a domain organization that is much different from the other Rho GTPases (19). The RhoBTBs contain, in addition to their N-terminal GTPase domains, two so-called Broad complex, Tramtrack, Bric-à-brac/Poxvirus, and zinc finger domains (29). This type of domain was first identified in a number of Drosophila gene products but were subsequently found in several proteins from yeast to man. Typically, this domain is found in proteins with functions in the regulation of transcription and differentiation; however, the currently available literature does not ascribe any obvious cellular function for RhoBTB (19).

To the best of our knowledge, none of the Rho GTPases have been implicated in mitochondrial homeostasis, and in this respect Miro constitutes a subfamily of Rho GTPases with unique properties. Interestingly, another group of proteins, unrelated to the Rho GTPases but with GTP-binding properties, has been shown to have roles in mitochondrial homeostasis. Work in budding yeast, D. melanogaster and C. elegans, as well as in mammalian cells has identified three conserved GTPases in this process: Fzo/mitofusin, Dnm1/Drp1/Dlp1, and Mgm1/Opa1/MspI (30, 31). Fzo/mitofusin is a transmembrane protein residing on the outside of mitochondria where it regulates fusion of the outer mitochondrial membrane during mitochondrial fusion (29). The converse process is regulated by Dnm1/Drp1/Dlp1. This protein bears resemblance to dynamins and regulates outer mitochondrial membrane fission (31). Yet another dynamin-like molecule, Mgm1/Opa1/MspI, seems to regulate several aspects of inner mitochondrial membrane remodeling. Interestingly, overexpression of a constitutively active mutant form of the mitofusin Mfn2 in COS 7 cells resulted in the formation of a continuous mitochondrial network, presumably caused by a shift in the balance of the mitochondrial dynamics favoring an over activity of mitochondrial fusions (32). This observation may relate to the mitochondrial aggregates seen in cells expressing constitutively active Miro-1/Val-13. Thus, these aggregates might be caused by an increase in mitochondrial membrane fusion; however, the exact mechanisms for remains to be studied.

Mitochondrial homeostasis is a vital cellular process, and alterations in the intracellular environment that affect mitochondrial function normally triggers the apoptosis program (23). In this respect, the mitochondria are functioning as cellular sensors that, upon the receipt of proapoptotic signals, release factors such as apoptosis-inducing factor and cytochrome c into the cytoplasm, which assist in inducing the caspase cascade (23, 24). Rho GTPases have been implicated in various aspects of apoptosis (reviewed in Ref. 13). For example, Cdc42 is necessary for the nerve growth factor withdrawal-induced apoptosis of neuronal cells (33). In addition, Cdc42 is a caspase substrate, and mutation of the caspase cleavage site results in a marked protection against Fas ligand-induced apoptosis (34). Ectopic expression of the constitutively active mutant Miro-1/Val-13 in COS 7 cells increased the apoptotic rate of expressing cells. The exact molecular mechanism for this remains to be studied, but one possible causative might be the altered mitochondrial properties, seen as the aggregation of the mitochondrial network. Interestingly, it has been shown that interference of the activity of Dnm1/Drp1/Dlp1 in such a way that the mitochondrial fission was inhibited resulted in decreased apoptosis (35), further emphasizing the importance for a correct balance between mitochondrial fusion and fission for cell viability.

We have identified an evolutionarily conserved family of Rho GTPases, Miro, with atypical domain organization and unique cellular properties. Experiments employing NIH3T3 fibroblasts and COS 7 cells indicated that Miro is a constituent of mitochondria and that the protein is involved in the regulation of mitochondrial homeostasis and apoptosis.

    FOOTNOTES

* This work was supported in part by grants from the Swedish Cancer Society (to P. A.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed. Tel.: 46-18-160408; Fax: 46-18-160420; E-mail: pontus.aspenstrom@LICR.uu.se.

Published, JBC Papers in Press, December 12, 2002, DOI 10.1074/jbc.M208609200

    ABBREVIATIONS

The abbreviations used are: GDI, guanine nucleotide dissociation inhibitor; EST, expressed sequence tag; FITC, fluorescein isothiocyanate; JNK/SAPK, Jun N-terminal kinase/stress-activated protein kinase; TRITC, tetramethylrhodamine isothiocyanate; DAPI, 4',6-diamidino-2-phenyindole; FCS, fetal calf serum; PBS, phosphate-buffered saline; Z, benzyloxycarbonyl; FMK, fluoromethyl ketone.

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

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