Mismatch Uracil Glycosylase from Escherichia coli

A GENERAL MISMATCH OR A SPECIFIC DNA GLYCOSYLASE?*

Rory J. O'Neill {ddagger}, Olga V. Vorob'eva §, Hassan Shahbakhti {ddagger}, Erik Zmuda ¶, Ashok S. Bhagwat § and Geoffrey S. Baldwin {ddagger} ||

From the {ddagger}Imperial College London, Department of Biological Sciences, Sir Alexander Flemming Building, South Kensington, London SW7 2AZ, United Kingdom, the §463 Chemistry Building, Department of Chemistry, Wayne State University, Detroit, Michigan 48202-3489, and Trevigen, Gaithersburg, Maryland 20877

Received for publication, October 23, 2002 , and in revised form, March 27, 2003.


    ABSTRACT
 TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 CONCLUSIONS
 REFERENCES
 
The gene for the mismatch-specific uracil glycosylase (MUG) was identified in the Escherichia coli genome as a sequence homolog of the mammalian thymine DNA glycosylase, with activity against uracil in U·G mismatches. Subsequently, 3,N4-ethenocytosine ({epsilon}C), thymine, 5-hydroxymethyluracil, and 8-(hydroxymethyl)-3,N4-ethenocytosine have been proposed as possible substrates for this enzyme. The evaluation of various DNA adducts as substrates is complicated by the biphasic nature of the kinetics of this enzyme. Our results demonstrate that product release by the enzyme is very slow and hence comparing the "steady-state" parameters of the enzyme for different substrates is of limited use. Consequently, the ability of the enzyme to excise a variety of damage products of purines and pyrimidines was studied under single turnover conditions. Although the enzyme excised both {epsilon}C and U from DNA, the former adduct was significantly better as a substrate in terms of binding and hydrolysis. Some products of oxidative and alkylation damage are also moderately good substrates for the enzyme, but thymine is a poor substrate. This comparison of different substrates under single turnover conditions provides a rational basis for comparing substrates of MUG and we relate these conclusions to the known crystal structures of the enzyme and its catalytic mechanism.


    INTRODUCTION
 TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 CONCLUSIONS
 REFERENCES
 
DNA glycosylases excise damaged bases from DNA and prevent mutations. They act upon a wide range of DNA adducts that result from the action of a number of DNA damaging agents including water, reactive oxygen species, and alkylating agents. The modified bases that are removed in this way include the products of hydrolytic deamination of cytosine (product: uracil (U)) and 5-methylcytosine (T); the oxidative damage products, 8-oxoguanine and 5-hydroxycytosine (5-OHC)1; and the alkylation products, 3-methyladenine and 3,N4-ethenocytosine ({epsilon}C). While glycosylases such as uracil DNA glycosylase (UDG) are very specific regarding their substrate (1), others, such as endonuclease III, can excise a wide variety of damaged DNA bases (2).

The Escherichia coli mismatch-specific uracil glycosylase enzyme (MUG), which was identified as a sequence homolog of human thymine DNA glycosylase (TDG), was so named because it was seen to excise U from a U·G pair (3). It was subsequently shown to also excise {epsilon}C and T (4, 5). The crystal structure of MUG revealed that, despite a lack of sequence homology, there was a significant structural homology to UDG (5). However, the amino acid residues that confer such a high degree of specificity in UDG were altered in MUG in such a way as to remove any specificity from the active site pocket, which provides a rational basis for its broad substrate specificity (6). It appears to gain its specificity from specific interactions with the widowed G in the opposite strand instead (5, 6).

Although MUG has been characterized as being active against U, T, and {epsilon}C, it is not yet clear whether these are the true substrates for the enzyme. This uncertainty exists partly because a mug mutant does not have a mutator phenotype in dividing E. coli, and in stationary phase cells, where it is expressed well, it is only a modest mutator (7, 8). It is possible that this lack of a strong phenotype is caused by the presence of alternative enzymes in E. coli that process the promutagenic lesion U and the T·G mispair. Uracils are efficiently removed from DNA by UDG (9) and T·G mismatches are repaired by a separate repair pathway called very short patch repair (10, 11). Additionally, there is no evidence, as yet, that E. coli ever generates {epsilon}C in its DNA (12).

Therefore, it is possible that the biologically important substrate of MUG is something other than U, T, or {epsilon}C. There have been recent reports suggesting that MUG may process a 5-hydroxymethyluracil (13), 1,N2-ethenoguanine (14), and 8-(hydroxymethyl)-3,N4-ethenocytosine (15). However, these reports either do not contain quantitative comparisons between relative preferences of the enzyme for different substrates or report apparent steady-state rates of reactions. The latter are problematic, since we demonstrate here that MUG is severely inhibited by its dissociation from abasic product DNA. Hence it is difficult to assess whether these are likely in vivo targets for MUG.

If MUG does process a broad diversity of DNA lesions, then it is possible that it acts as a general mismatch DNA glycosylase removing a variety of different lesions that accumulate during the late stationary phase. In this study we have tested this hypothesis by investigating the activity of MUG with a variety of different substrates. We also demonstrate that the use of single turnover kinetics provides a rational means for comparing the rates of nucleotide hydrolysis by MUG.

The bases that we have investigated represent different types of damage from a wide variety of possible sources: these include the products of redox reactions, alkylation, and deamination. Our results show that, under the right conditions, MUG can process a broad spectrum of DNA adducts.


    MATERIALS AND METHODS
 TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 CONCLUSIONS
 REFERENCES
 
Protein Purification—The mismatch uracil glycosylase from E. coli (MUG) was overexpressed from a construct of the wild type mug gene in the vector pTrc99A (supplied by Dr R. Savva (Birkbeck, London)) in E. coli strain BL834. An overnight culture of the strain carrying this construct was diluted 1000x into LB broth and grown overnight at 37 °C before induction with 1 mM isopropyl-1-thio-{beta}-D-galactopyranoside. The cultures were grown for a further 5 h at 37 °C before the cells were harvested. The harvested cells were lysed by sonication in buffer A (20 mM Tris-HCl, pH 8.0, 10 mM EDTA) with "Complete" protease inhibitors (Roche Molecular Biochemicals). The lysate was incubated with 1/10 volume of 10% (w/v) streptomycin sulfate for 1 h on ice before centrifugation at 50,000 relative centrifugal force for 1 h to remove the cell debris. The supernatant was loaded onto a DEAE-cellulose column, equilibrated in buffer A, and the flow-through was run directly onto an SP-Sepharose column, also equilibrated in buffer A. The connected columns were washed with 400 ml buffer A; the DEAE column was then removed and the SP Sepharose column washed with a further 150 ml of buffer A. Protein was eluted from the column using a gradient of 0–100% buffer B (20 mM Tris-HCl, pH 8.0, 10 mM EDTA, and 1 M NaCl) over 400 ml. Fractions of the eluate were analyzed by SDS-PAGE and those fractions containing MUG were pooled and the volume reduced by ultrafiltration in a stirred cell. The partially purified MUG protein was loaded onto a Superdex-75 column, equilibrated in buffer C (20 mM Tris-HCl, pH 8.0, 10 mM EDTA, and 200 M NaCl), and eluted in the same buffer. Fractions were assayed, as above, and those containing pure MUG were pooled and concentrated by ultrafiltration. The concentration of the protein was calculated from the OD280, based on an extinction coefficient of 25, 590 M1 cm1. Glycerol was added to 20% (v/v), and aliquots of the enzyme were snap-frozen and stored at–20 °C for up to 2 months.

Oligonucleotide Synthesis and Purification—All of the damaged nucleotides tested in this study were incorporated into the same oligonucleotide sequence (5'-GAC TAA XAA TGA CTG CG, where X is the modified nucleotide) by Cruachem (Glasgow, UK). Modified oligonucleotides were synthesized using fast phosphoramidite chemistry and mild de-protection conditions. All oligonucleotides were purified by reverse phase HPLC, as described previously (16). Double-stranded substrates were made by annealing the modified nucleotide containing strand with an equimolar amount of the complimentary strand containing either a G or T opposite the damage site (5'-CG CAG TCA TT(G/T) TTA GTC), which was synthesized by Cruachem and purified as above. The strands were annealed by heating to 90 °C and slowly cooling to room temperature. The double-stranded substrates are referred to as X·G, where X is the modified base: the complete range of substrates tested and the sources of the modified bases are shown in Table I.


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TABLE I
Substrates

 

Enzyme Assays—Oligonucleotide substrates were 5'-labeled with 32P on the modified strand prior to annealing. The 32P-labeled duplex substrates, typically 100 nM, were reacted at 25 °C with MUG in reaction buffer (50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM EDTA, and 0.1 mg ml1 bovine serum albumin): these are the standard reactions and apply to all assays unless other conditions are stated. At selected time points 10-µl samples were removed and quenched with 10-µl aliquots of 0.1 M NaOH. The quenched samples were then heated to 90 °C for 20 min, to cleave the abasic site products. An equal volume of formamide loading buffer (95% formamide, 0.04% bromphenol blue, 0.04% xylene cyanol, and 20 mM EDTA) was added, and subsequently 38 µl of the heat-denatured samples were separated by denaturing 18% PAGE. The formation of product was quantified using a Fuji phosphorimager and the reaction rate determined by fitting the data to the stated kinetic equations using Grafit 5 (Erithacus Software).

MUG reaction assays requiring a short time course (53 ms to 100 s) were performed using a HI-TECH RQF-63 rapid quench flow. The quenched samples were analyzed as described above.


    RESULTS
 TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 CONCLUSIONS
 REFERENCES
 
Investigation of MUG Assay Conditions—We used a double-stranded substrate in which one strand contained a single modified DNA base. For comparison, all modified bases have been investigated as a mismatch with a guanine residue, since the crystal structure of MUG with DNA shows that the enzyme makes specific contacts with the guanine opposite the lesion (5). However, {epsilon}A and inosine lesions would normally be paired with thymine under physiological conditions. Therefore, we have also analyzed the {epsilon}A and inosine substrates as mismatches with a thymine residue.

Since the excision of bases by MUG was seen to be slow, it was essential to first establish conditions under which the enzyme remained active for long time periods so that slow rate constants could be accurately determined. To do this, the enzyme was first preincubated in the reaction buffer at either 25 or 37 °C for increasing time periods prior to the addition of substrate containing a U·G mismatch. Following the addition of substrate, the reaction was allowed to proceed for a fixed time period of 30 min before quenching with NaOH. It was first established that this was sufficient to cleave >95% of this particular substrate at either temperature. Preincubation times were varied from 30 min to 72 h. These reactions revealed that, at 37 °C, the activity of MUG decreased rapidly, and after 3 h it was no longer able to convert all of the substrate to product within the time given (Fig. 1A). However, at 25 °C full enzyme activity was observed up to 48 h, and a reduction in activity was only observed after 72 h (Fig. 1B). All subsequent assays with MUG were therefore performed at 25 °C and with time courses of up to 48 h to ensure that the observed rates of cleavage were as accurate as possible.



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FIG. 1.
MUG enzyme (5 µM) was incubated in reaction buffer, for increasing time periods shown at 37 °C (A) and 25 °C (B). A reaction was then initiated by adding 100 nM U·G substrate. The reaction was allowed to proceed for 30 min before quenching with NaOH and analyzing by denaturing PAGE.

 

Steady-state Reactions with MUG—To determine the rate of reaction of MUG under steady-state conditions, reactions were performed with a 10-fold excess of U·G substrate (Table I) over enzyme for up to 48 h. At time points past the first few hours there was a linear increase in product with time (Fig. 2). The gradient of this steady-state phase gave a rate of 3.5 x 104 S–1. Notably, however, the linear fit of the data did not pass through the early data points or the origin, but had an intercept of 0.1 µM. This corresponds to the enzyme concentration used in the reaction, and hence the early reaction times are indicative of a burst phase, where the enzyme rapidly binds and cleaves an equal amount of substrate, but where subsequent turnovers are limited by the very slow release of product. Thus the steady-state phase of the reaction is not indicative of the true catalytic abilities of the enzyme. To obtain a better understanding of the substrate specificity of the enzyme, reactions were performed under single turnover conditions where the accumulation of product gives a measure of the actual chemical cleavage step that is not affected by the limited release of product.



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FIG. 2.
MUG enzyme (0.1 µM) was incubated with a 10-fold excess of substrate under standard reaction conditions. The solid line is the best fit to a linear equation for the data points ranging from 1.5 x 103 to 1.6 x 105 s, with a rate of 3.5 x 104 ± 7 x 106 s1.

 

Single Turnover Reactions with MUG—The rate of substrate cleavage by MUG was investigated directly by performing reactions with an excess of enzyme over substrate. Under these conditions only a single turnover of the reaction will occur and the accumulation of product will thus correspond directly to the rate of N-glycosyl bond hydrolysis under the conditions tested. Reactions were initially performed with the U·G, {epsilon}C·G, and T·G DNAs, which have already been identified as substrates for MUG in vitro (4, 5). The reactions with U·G and {epsilon}C·G proceeded much faster than the steady-state reaction and a quench flow machine was utilized to accurately perform the quench assay over shorter time scales. Both of these substrates showed a single exponential increase in product with cleavage rates that are at least 2 orders of magnitude faster than the steady-state cleavage rate with U·G (Fig. 3, A and B). However, the T·G substrate was cleaved much more slowly, and after 11 h only ~10% of the substrate had been cleaved (Fig. 3C).



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FIG. 3.
MUG enzyme (5 µM) was reacted with double-stranded substrate (0.1 µM) under standard reaction conditions. A rapid quench flow device was used to take samples at time points ranging from 53 ms to 100 s in A and B. A, data for the {epsilon}C·G substrate is shown with the best fit to a single exponential with a rate of 0.18 ± 0.006 s1. B, data for the U·G substrate is shown with the best fit to a single exponential with a rate of 0.037 ± 0.002 s1. C, data for the T·G substrate is shown with the best fit to a linear equation, which gives an initial rate of 2.7 x 106 ± 1 x 107 s1.

 

The single turnover reaction with the U·G substrate confirms that the actual cleavage of this substrate by MUG is 100-fold faster than the turnover rate, which will thus be limited by the dissociation of product (cf. Figs. 2 and 3B). The product inhibition observed with this enzyme is so severe that the enzyme is effectively a single turnover enzyme, and it is therefore not possible to determine the classical catalytic constants kcat and Km for MUG. All subsequent assays with MUG were therefore performed under single turnover conditions.

Concentration Dependence of Single Turnover Rates—Even with single turnover reactions, the observed rate of substrate cleavage can vary with enzyme concentration if the initial enzyme-substrate complex is not saturated under the conditions used. To test whether the observed reaction rates were sensitive to enzyme concentration, a series of single turnover reactions were performed with substrates U·G and {epsilon}C·G and increasing concentrations of MUG. Reactions with the U·G substrate did indeed show an increase in rate with enzyme concentration, which was found to fit a hyperbolic dependence that gave a saturating cleavage rate (kcl) of 0.04 s1 and an apparent Kd of 0.42 µM (Fig. 4A).



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FIG. 4.
Single turnover reactions were performed with 0.1 µM substrate and increasing concentrations of MUG ranging from 0.2 to 8.0 µM using a rapid quench flow device. The single exponential rate of product formation was determined, as in Fig. 3, and the rates are plotted here against enzyme concentration. A, data for the U·G substrate is here shown with the best fit to a hyperbolic equation with saturating cleavage rate (kcl) of 0.040 ± 0.001 s1 and an apparent Kd of 0.42 ± 0.03 µM. B, data for the {epsilon}C·G substrate.

 

The rate dependence of the {epsilon}C·G substrate by MUG showed a very different behavior to the U·G substrate: all of the rates determined at each concentration were within experimental error of each other and no hyperbolic dependence on the enzyme concentration was observed (Fig. 4B). It was not possible to determine the apparent Kd for this substrate, which was too low to measure from these data. The conclusion, therefore, is that MUG was saturated with the {epsilon}C·G substrate at the lowest concentration tested. There is thus a significant difference in the affinity of MUG for these two substrates.

Alternative Substrate Cleavage by MUG—The activity of MUG was tested with a series of potential substrates to investigate the possible in vivo targets of the enzyme. The potential substrates were incorporated into oligonucleotides with the same sequence context as the U·G substrate. In addition, the {epsilon}A and inosine bases were also incorporated opposite a T, which is more representative of physiological conditions (see "Materials and Methods"). Reactions were performed under single turnover conditions, and the accumulation of product was monitored over time. Some of the substrates tested were unstable when stored and the reactions did not reach 100% completion. Deviations due to substrate degradation were minimized by performing the reactions with substrate freshly purified by HPLC.

To make valid comparisons between the cleavage rates of MUG with different substrates, it is necessary to perform the reactions under saturating conditions. It was not feasible to do a concentration rate profile with these other substrates, since the rates were too low. Instead, we performed the assays with two enzyme concentrations (5 and 10 µM), which we had shown to be saturating with the U·G and {epsilon}C·G substrates (Fig. 4). If the reaction rates did not change, then we could assume that the reaction was saturating and that the observed rate (kobs) was equivalent to the catalytic cleavage rate (kcl). But, if the rates were dependent on enzyme concentration, then we could assume that these reactions were not saturating even under these extreme conditions of enzyme excess.

In most instances, the accumulation of product followed a single exponential increase and could be fitted to provide a rate constant for the cleavage reaction. However, the reactions with the T·G (Fig. 3C) OHC·G, {epsilon}A·T, and I·T substrates (data not shown) were so slow that they did not reach a discernable end point. In these cases the data points were fitted to a linear equation and the gradient was used to establish an initial rate.

The cleavage rates of the different substrates at 5 and 10 µM MUG are shown in Table II. Of the substrates that cleaved, all were at saturating rates under the conditions tested, with the exception of {epsilon}A and inosine. The observed rates of cleavage vary over 6 orders of magnitude, and {epsilon}C was the best substrate for the enzyme. While a wide variety of modified DNA bases were excised by MUG, the enzyme does not cleave all potential base adducts. For three of the substrates tested, no excision activity could be detected.


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TABLE II
Rates of substrate cleavage by MUG

 

Activity against Single-stranded DNA—MUG is sometimes referred to as double-strand specific uracil glycosylase (17), and the crystal structure suggests a key role for the opposite strand in substrate recognition (5). To verify the importance of this, all of the substrates toward which the enzyme exhibited activity were also tested in their single stranded form. Reactions were performed under the same single turnover conditions that were used for the double stranded substrates. Cleavage activity by MUG was detected only with the three substrates that had the highest activity in double stranded form. When the reaction was performed with 10 µM MUG and 100 nM substrate, {epsilon}C was cleaved from single stranded DNA with a rate of 1.1 x 104 s1 (Fig. 5), lowering the enzyme concentration to 5 µM did not alter the rate of reaction (1.2 x 104 s1; data not shown), although reducing the enzyme concentration to 2 µM resulted in a halving of the rate (0.47 x 104 s1; data not shown). Reactions with uracil and 5-hydroxyuracil did not reach completion after 48 h, but initial cleavage rates with 5 µM MUG were 9.0 x 107 s1 and 7.0 x 107 s1, respectively (data not shown).



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FIG. 5.
MUG enzyme (10 µM) was reacted with single-stranded ethenocytosine substrate (0.1 µM) under standard reaction conditions. The data are shown with the best fit to a single exponential with a rate of 1.1 x 104 s1.

 


    DISCUSSION
 TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 CONCLUSIONS
 REFERENCES
 
Kinetics of Base Excision—In this study we have examined the kinetics of base excision by MUG. Having first established conditions under which MUG is active for up to 48 h (Fig. 1), we were able to perform reactions over long time courses and accurately determine cleavage rates. Analysis of U·G cleavage under steady-state conditions revealed a burst phase followed by a very slow steady-state phase (Fig. 2). Subsequent analysis of the same substrate under single turnover conditions showed that N-glycosyl hydrolysis proceeds 100-fold faster than the turnover rate (Fig. 4). Similar biphasic behavior has been observed with other DNA glycosylases (18, 19, 20), and it is clear that the turnover of MUG is also inhibited by tight binding to its product.

(Eq.1)

The turnover of MUG is limited by the rate of product dissociation: koff in Equation 1 above. Under single turnover conditions, when the substrate is saturated with enzyme, the observed rate will be equal to kcl, the chemical rate of hydrolysis. Under non-saturating conditions, the observed rate will be limited by the partial saturation of the precatalytic enzyme-substrate complex (ES). The {epsilon}C·G substrate saturates at much lower enzyme concentrations than the U·G substrate (Fig. 4). This is a reflection of the different binding characteristics of the two substrates: MUG binds ethenocytosine much more tightly than uracil.

The tighter binding of the {epsilon}C containing substrate by MUG probably also accounts for its activity against single-stranded DNA containing {epsilon}C. Cleavage of the single-stranded substrate is 1500-fold slower than the double-stranded substrate (cf. Figs. 3 and 5), although MUG must have a higher Kd for {epsilon}C in the single-stranded substrate, since it did not saturate at the lowest enzyme concentrations used. This demonstrates the importance of interactions with the second DNA strand, although interactions with the ethenocytosine are sufficient to produce some excision activity when it is present in a single DNA strand. However, with the weaker binding uracil, the enzyme activity against the single DNA strand was so low that it is not likely to be of any significance.

The activity of MUG in vivo may well be modulated by the action of other enzymes. It has been shown that E. coli endonuclease IV stimulates MUG reactions in vitro (8, 17), presumably by accelerating product release. Furthermore, the MutY glycosylase is also stimulated by endonuclease IV (21), and the human glycosylases TDG and OGG1 have been shown to be stimulated by the human AP endonuclease (22, 23). In vivo, where the process of base excision is expected to be coupled with abasic endonucleolytic cleavage, product release may not be the rate-limiting step. When considering the activity of MUG with different substrates in vitro, it is thus more informative to compare single turnover rates, as this gives a clearer indication of the relative activity of MUG with different substrates.

Of the different substrates tested here, a duplex containing the {epsilon}C·G pair had the highest affinity for the enzyme and was cleaved with kcl of 0.18 s1 (Table II). While U·G was the next best substrate, substantially higher amounts of enzyme were required to saturate it (Fig. 4), and kcl was ~5-fold lower than for {epsilon}C·G (kcl 0.037 s1; Table II). Of the other substrates tested, 5-hydroxyuracil (5-OHU), {epsilon}A, and inosine (hypoxanthine) had observed cleavage rates 2–3 orders of magnitude slower than {epsilon}C·G (Table II); thymine, 5-hydroxymethyluracil, and 5-hydroxycytosine were cleaved to some extent, although extremely slowly; and the enzyme did not demonstrate any detectable activity toward 5-hydroxymethylcytosine, 5,6-dihydrouracil, and 5,6-dihydrocytosine. Inosine and {epsilon}A were the only substrates tested that did not reach saturation under the conditions tested and, notably, when paired with the more biologically relevant T, the observed rate dropped a further 2 orders of magnitude. It is possible that, in vivo, the enzyme may have different substrate selectivity, act on other sequence contexts, or be modified by the presence of accessory factors. However, based on the results presented here, these other base modifications cannot be considered to be the preferred substrates for the enzyme.

Previous studies have reported that a variety of other nucleotide lesions are also substrates for MUG, including 5-hydroxymethyluracil (13), 1,N2-ethenoguanine (14), and 8-(hydroxymethyl)-3,N4-ethenocytosine (15). However, these reports either do not contain quantitative data or report apparent steady-state rates of reactions. The use of steady-state conditions to study MUG and other product inhibited DNA glycosylases leads to an underestimation of reaction rates of the best substrates and consequently makes the poorer substrates appear better than they really are. The reported kcat value for {epsilon}C is only ~2200-fold higher than that for T (4), while the kcl value for {epsilon}C·G is ~65,000 times the observed cleavage rate for T·G (Table II). This is in large part due the fact that the reported kcat value for {epsilon}C is lower than the kcl value by a factor of ~11. It is thus difficult from these reports to assess the relative efficiencies with which MUG is able to act upon these different substrates and hence whether they are likely in vivo targets for the enzyme.

Accommodation of Modified Nucleotides—There are notable trends that can be gleaned from the observed activities of MUG toward different substrates. The bulkiness at the 5-position has a dramatic effect on the activity of MUG. This trend can be clearly seen when comparing U·G with OHU·G, which has 10-fold lower activity and OHMeU·G, which has four orders of magnitude lower activity. However, OHMeU·G is a better substrate than T·G (5-methly-U), even though it is larger. It has recently been reported that MUG activity is dependent on the electron withdrawing capability of the 5-substituent, rather than simple steric exclusion (24). This is consistent with a dissociative mechanism of cleavage with a uracil anion leaving group, as proposed for UDG (25, 26), and the uncatalyzed cleavage of deoxyuridine derivatives (27). Our data support the view that there is a balance between the size of any 5-substituent and its electron withdrawing capability on the activity of MUG.

In contrast to the poor activity of 5-substituted nucleotides, substrates that have bulky substituents on the 3- and 4-positions are, in many cases, good substrates. Most notable in this respect is ethenocytosine, which is the best substrate tested. But, MUG is also able to cleave the purines inosine and {epsilon}A. Modeling of {epsilon}C into the active site of MUG has demonstrated that the active site has considerable space to accommodate bulky adducts on this face of the nucleotide (6). The results presented here, and elsewhere (14, 15), demonstrate that these interactions are not completely specific to {epsilon}C and that even more bulky adducts can be accommodated by MUG.

It is also of note that, with the exception of ethenocytosine, all other cytosine derivatives are poor substrates: a comparison of OHU·G and OHC·G reveals a difference of 4 orders of magnitude in the cleavage rate. In addition, N4-methyl and N4-ethyl cytosine containing DNAs were not substrates for MUG.2 The only structural difference between these nucleotides is the presence of a keto oxygen or amino group at the 4-position (in ethenocytosine this amide is alkylated to form a ring with N3). Furthermore, the analysis of the single turnover cleavage rate with concentration for the U·G and {epsilon}C·G substrates (Fig. 4) reveals a difference in the affinity of the enzyme for these different substrates. Notwithstanding, there is still a difference in the hydrolysis rate of these different substrates by MUG, even under saturating conditions. The variations in cleavage rate may be due to subtle differences in the arrangement of the catalytic residues as the active site adapts to accommodate structurally different substrates. Alternatively, these differences could be due to the electronic inductive properties of the different nucleotides.

In the active site pocket of UDG, an Asn residue is thought to form specific interactions with the Watson-Crick base paring functions of the target uracil (1). In MUG, however, there are no specific interactions to discriminate between the N4 of cytosine or the O4 of uracil (6). Alternatively, the electronic inductive properties of the 4-substituent may provide discrimination: a 4-carbonyl group would stabilize a departing anion, whereas a 4-amino group would destabilize the anion, reducing cleavage rate (Fig. 6). This is presumably why cytosine is inherently a more stable base than thymine: hydrolysis of unprotonated T will occur, but hydrolysis of unprotonated C will not (28). The relative rates of cleavage of different substrates by MUG are consistent with the hydrolysis reaction proceeding via a dissociative mechanism with a departing uracil anion. This type of mechanism has been attributed to the UDG enzyme (25, 26), and it seems likely that it also applies to MUG.



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FIG. 6.
A, the anion of uracil will be stabilized by delocalization of the negative charge to the O2 and O4 oxygen atoms, as shown. B, the anion of cytosine cannot be stabilized by delocalization, since the N4 amino group has a propensity to donate electrons to the pyrimidines ring, as represented by the keto-enol tautomerization, which does not stabilize the anion, as shown.

 


    CONCLUSIONS
 TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 CONCLUSIONS
 REFERENCES
 
In this study we have used a kinetic approach to investigate the action of MUG with a broad range of DNA damage lesions. We have shown that the use of single turnover kinetics provides a rational basis for comparing the cleavage rates of different substrates and avoids misinterpretations due to the slow release of MUG from its product. The best substrate for MUG is ethenocytosine and it may be that this is the biologically relevant substrate. The biology of MUG suggests that it may be active in stationary phase (8), and hence it is possible that E. coli DNA accumulates {epsilon}C during this stage of cell growth.

However, previous studies have failed to isolate {epsilon}C from the DNA of E. coli (12). But, the mutator phenotype of mug was only apparent after 2 days of the cells being in stationary phase (8), and it is unlikely that, when attempting to isolate {epsilon}C from E. coli, the cells were starved for several days prior to the preparation of chromosomal DNA. Alternatively, it could be that MUG efficiently repairs {epsilon}C lesions in vivo, so that it is difficult to detect any residual {epsilon}C in the DNA from mug+ cells used for that work. Thus, the question of whether {epsilon}C is the biologically relevant substrate for MUG can only be resolved by the careful quantitation of DNA adducts in late stationary phase mug E. coli cells.

The other DNA damage lesions that we have tested here have a significantly lower activity against MUG than {epsilon}C and, to a lesser extent, uracil. It therefore seems unlikely that MUG acts as a general X·G mismatch glycosylase in vivo. However, since MUG appears to be active only in the late stationary phase (8), the rate of reaction may not be critical to the biological relevance of this enzyme. If {epsilon}C is not found to accumulate in late stationary phase E. coli, then it remains possible that MUG acts as a general X·G glycosylase and repairs a wide range of DNA damage lesions, caused by oxidation and other cellular processes, such as those characterized here. Alternatively, MUG may be predominantly active against another DNA lesion that we have not investigated.


    FOOTNOTES
 
* This work was supported by the Biotechnology and Biological Sciences Research Council (UK) and the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Back

|| To whom correspondence should be addressed. Tel.: 44-20-7594-5288; Fax: 44-20-7584-2056; E-mail: g.baldwin{at}ic.ac.uk.

1 The abbreviations used are: OHC, hydroxycytosine; UDG, uracil DNA glycosylase; MUG, mismatch-specific uracil glycosylase; TDG, thymine DNA glycosylase; {epsilon}C, 3,N4-ethenocytosine; HPLC, high performance liquid chromatography; OHU, hydroxyuracil. Back

2 O. V. Vorob'eva and A. S. Bhagwat, unpublished observations. Back


    ACKNOWLEDGMENTS
 
We are grateful to Tim Waters (University College London) for the deoxyethenocytidine phosphoramidite and Renos Savva (Birkbeck College, London) and Hassan Shahbakhti and Kuakarun Krusong (Imperial College London) for their general help and advice. A. S. B. thanks Katherine Wood (Trevigen) and Elena Kubareva (Moscow State University) for useful discussions and support.



    REFERENCES
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 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 CONCLUSIONS
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