From the Department of Bacteriology, Swedish
Institute for Infectious Disease Control, 171 82 Solna, Sweden, the
§ Microbiology and Tumor Biology Center, Karolinska
Institutet, 171 77 Stockholm, Sweden, the ¶ Department of Medicine
II, Technical University of Munich, Ismanigerstr. 22, 816 75 Munich,
Germany, and
Pyrosequencing AB, Vallong. 1, 752 28 Uppsala, Sweden
Received for publication, December 9, 2002, and in revised form, January 21, 2003
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ABSTRACT |
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The gastric pathogen Helicobacter
pylori harbors one Nudix hydrolase, NudA, that belongs to the
nucleoside polyphosphate hydrolase subgroup. In this work, the
enzymatic activity of purified recombinant NudA protein was
analyzed on a number of nucleoside polyphosphates. This
predicted 18.6-kDa protein preferably hydrolyzes diadenosine tetraphosphate, Ap4A at a kcat of
0.15 s The nucleoside diphosphate linked to some
other moiety X (Nudix) protein family, formerly called the
MutT protein family, consists of about 800 proteins in more than 200 species from all kingdoms (1). This group of proteins may be divided
into subfamilies based on the substrate specificity: ADP-ribose,
dinucleoside polyphosphates, NADH, nucleotide sugars, or ribo- and
deoxyribonucleoside triphosphates (2). The active site of Nudix
hydrolases corresponds to the consensus sequence
GX5EX7REUXEEXGU (where
X represents any amino acid and U represents Ile, Leu, or Val).
Although enzyme activities of Nudix proteins have been studied
extensively, the biological role of these enzymes is still poorly
understood (3-6). It has been proposed that Nudix hydrolases are
housecleaning enzymes that decrease the intracellular level of toxic
substances and cell signaling molecules, e.g. dinucleoside polyphosphates (7). The dinucleoside polyphosphate
Ap4A1 is induced
upon oxidative stress and heat shock both in prokaryotes and eukaryotes
(8, 9), and the Nudix enzyme subsequently degrades this signaling
component to restore the intracellular balance. Experimental data
indicate that an increased concentration of Ap4A is a
signal for cell division in Escherichia coli (10). Furthermore, it has been suggested that Ap4A and
Ap3A are physiological antagonists. Ap4A
induces apoptosis, and Ap3A is a co-inducer of
differentiation in both murine and human cells (11).
Nudix enzymes have also been suggested to play a role in bacterial
invasion of eukaryotic cells. The Bartonella bacilliformis Nudix hydrolase, encoded by the ialA gene, was shown to be
associated with the ability to invade human erythrocytes (12). An
up-regulation of the expression of the E. coli K1 orthologue
ygdP during invasion of human brain microvascular
endothelial cells has also been demonstrated (13). Recently, it was
shown that transcription of the Rickettsia prowazekii
orthologue invA is temporarily increased during the early
stages of infection, similar to the stress-related protein GroEL
(14).
Previously, Nudix hydrolases were referred to as MutT proteins since
the first studied protein in this group was MutT in E. coli.
MutT hydrolyzes 8-oxo-dGTP, a compound generating A-T/C-G transversions, and thereby prevents DNA damage caused by oxidative stress (15). The MutT nomenclature used for proteins containing Nudix
motifs was misleading since many of these proteins do not seem to
hydrolyze toxic compounds such as 8-oxo-dGTP. Recently, the idea of an
indirect involvement of E. coli MutT in the prevention of
DNA damage in vivo was questioned (16). In this study, it was shown that the incorporation of 8-oxo-dGTP into the genome by DNA
polymerase is rather inefficient in vitro at measurable intracellular concentrations. Furthermore, the mutT
phenotype was still observed at anaerobic conditions when no 8-oxo-dGTP is produced.
About half of the population of the world is colonized with the gastric
pathogen Helicobacter pylori, and the infection persists lifelong unless treated. This Gram-negative, microaerophilic bacterium causes gastritis, gastric ulcer, and duodenal ulcer and is associated with gastric cancer (17, 18). H. pylori is primarily an
extracellular bacterium, but it has been observed intracellularly,
although it is still unclear whether it is capable of multiplying and
persisting within host cells (19, 20). H. pylori is
transformed from a dividing rod to a nonculturable coccoid form at
unfavorable conditions, e.g. oxidative stress. The
intracellular level of 8-oxo-dGTP accumulates during this
transformation (21). The two fully sequenced strains of H. pylori harbor one Nudix hydrolase orthologue, Nudix
hydrolase A, nudA, with the gene numbers HP1228 in 26695 (22) and JHP1149 in J99 (23). Sequence analysis of the genome
suggests that the NudA protein is the only dinucleoside polyphosphate
hydrolase homologue present in this bacterium (2, 22, 23), which makes
H. pylori a suitable system to study the biological role of
Nudix hydrolases.
Because of the functional heterogeneity within this group of proteins,
the possible functions of H. pylori NudA are involvement in
(i) DNA repair through hydrolysis of 8-oxo-dGTP, (ii) oxidative stress
and/or heat shock response through degradation of stress signaling
molecules, or (iii) bacterial invasion of epithelial cells through
degradation of toxic substances induced during invasion. In this work,
we describe the enzymatic function of the Nudix hydrolase NudA in
H. pylori and use a constructed nudA insertion mutant to perform functional studies to determine the biological role
of this protein.
Bacterial Strains and Culture Conditions--
H.
pylori strains 67:21, CAG7:8, Ca73, Ca52, DU52:2, and Ca34 have
been characterized previously in our laboratory (24, 25). Additional
H. pylori strains used are: 26695 (22), J99 (23), Hp1 (26),
and 2808, a mouse-passed strain originating from a German patient
diagnosed with ulcer. All H. pylori strains were
grown on GC agar plates as described previously (24) or in Brucella
broth supplemented with 1% IsoVitaleX (BD Biosciences) and 5% fetal
bovine serum. Generation of the coccoid form of H. pylori
was obtained by storing a liquid bacterial culture in
phosphate-buffered saline for 40 days at 4 °C (without shaking).
E. coli strains DH5 PCR and Sequencing--
PCR was performed according to standard
protocols using Dynazyme DNA polymerase (Finnzyme, Espoo, Finland). DNA
cycle sequencing was performed using the BigDye kit, version 2 (Applied
Biosystems, Foster City, CA) and analyzed on an ABI 3100 apparatus.
Cloning, Expression, and Purification of Recombinant
NudA--
The H. pylori nudA gene was amplified with
PWO polymerase (Roche Molecular Biochemicals) using genomic DNA from
strain 67:21 as template and the forward primer 5'-CAT GCC ATG
GCA ATG CTA CAT AAA AAA-3' containing a NcoI site (in
bold) and reverse primer 5'-CCG CTC GAG TAA ATA
CCC CTC TCT TTT G-3' containing a XhoI site. The resulting
PCR fragment was cloned in-frame into the pET32a (+) vector,
transformed into DH5 Substrate Specificity Analysis Using HPLC--
The substrate
specificity was determined by ion-exchange high performance liquid
chromatography, HPLC (Äkta Basic, Amersham Biosciences). A
500-µl reaction mixture (50 mM Tris-HCl, pH 9.0, 100 µM substrate (Sigma), 10 mM MgCl2
with or without 1 µg of purified NudA) was incubated for 20 min at
37 °C, snap-frozen, and stored at Enzyme Assay for Catalytic Measurements--
The enzyme kinetic
constant, Km, for the preferred substrate
Ap4A was determined using an assay based on conversion of a
phosphatase-insensitive substrate to phosphatase-sensitive product (4).
The reaction mixture consisted of 50 mM Tris-HCl, pH 9.0, 2.5 mM Ap4A, 10 mM
MgCl2, 4 units of calf intestinal phosphatase, and 0.5 µg
of purified H. pylori NudA in a total volume of 50 µl. The
level of free phosphate, Pi, was assayed according to Ames
and Dubin (27).
Generation of a nudA Insertion Mutant--
The H. pylori
nudA gene with flanking regions was amplified using forward primer
5'-CTT TAA TGG CAA CC GAT GTT A-3' and reverse primer 5'-GGG ATG CAT
CAT CTC TCG CTT GTT AGG G-3' with genomic DNA from strain 67:21 as
template. The PCR fragment was cloned into the pCR2.1-TOPO vector
(Invitrogen) according to the manufacturer's instructions and
subcloned into pUC18 vector using the EcoRI sites. An
XhoI site (in bold) was generated seven codons
upstream of the Nudix box in the nudA gene using forward
primer 5'-GCG CAT AGA TAT TGC TAG CTC GAG GCA GTT CCC CCA
AGG-3' and reverse primer 5'-CCT TGG GGG AAC TGC CTC GAG CTA
GCA ATA TCT ATG CGC-3' in the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA). A kanamycin cassette from pJMK30 vector (28) was ligated into the XhoI site to interrupt the
nudA open reading frame. The insertion mutant,
2808nudA::Km, of the strain 2808 was generated
through natural transformation by addition of linearized vector
construct to the strain cultured in Brucella broth, allowing uptake and
homologous recombination. The mutant was confirmed by PCR using primers
flanking the construct (InvA2715 5'-TAT GGC TTT AGG CGT TTT AG-3',
InvA3861 5'-TCT ATG CCC ATA CCT TTC TA-3') and sequencing. The mutant
2808nudA::Km and the wild-type H. pylori strains were used for the functional analyses.
Mutation Frequency Analysis--
The frequency of spontaneous
mutations of the 2808nudA::Km and the wild type
were determined as described previously (29). Briefly, for each strain,
20 independent 1-ml cultures were grown in Brucella broth for 36 h. The total number of bacteria was calculated as the mean of three
tubes by plating serial dilutions of the culture on non-selecting
media. The number of rifampin-resistant H. pylori bacteria
was determined by plating onto GC agar plates containing 20 µg/ml (10 × minimal inhibitory concentration) rifampin. The
mutation frequency was calculated as the median number of rifampin-resistant bacteria divided by the mean colony-forming units
for each culture.
Oxidative and Heat Shock Stress Responses--
The mutant and
wild-type strains were cultured in Brucella broth to an
A600 of 0.2, equivalent to ~108
cells/ml, and aliquots of 3 ml were challenged with hydrogen peroxide
(H2O2) to a final concentration of 100 or 300 µM followed by a 2-h incubation without shaking. Samples
were collected in 30-min intervals, and viable counts were performed
before and during challenge from two parallel tubes. In the heat shock
experiment, 1 ml of each strain was transferred from 37 to 55 °C,
and samples were taken at different time points from 0 to 13 min.
Viable counts were performed before and after exposure.
Western Blot Analyses--
Bacteria were disrupted by sonication
on ice, and the cell debris was removed by centrifugation. Total
protein concentration was determined using the same method as above,
and 2 µg of each sample was separated by 10% SDS-PAGE and
transferred to polyvinylidene difluoride membrane (Bio-Rad). Primary
polyclonal rabbit antibodies raised against purified NudA (1:8,000,
Agrisera, Vännäs, Sweden) and horseradish
peroxidase-conjugated secondary goat anti-rabbit IgG antibodies
(1:8,000 DAKO, Glostrup, Denmark) were used. The signals were
visualized by the ECL detection system (Amersham Biosciences).
Sequence Comparison and Protein Prediction--
All the 70 H. pylori clinical isolates analyzed by PCR or hybridization
harbored the nudA gene (data not shown). The DNA sequence of
nudA was determined in four H. pylori strains,
67:21, Hp1, CAG7:8, and 2808, and aligned with the two fully sequenced
strains 26695 and J99 using the ClustalW program (supplemented data). H. pylori strains are known to display high sequence
diversity, and in the six strains, 48 of 465 nucleotides were variable,
corresponding to variations in 11 of 155 amino acids. Thus, 23% of the
substitutions were non-synonymous. Only one amino acid in the Nudix box
was variable, indicating conservation in this protein domain as expected.
The NudA protein in strain 26695 has a predicted size of 18.6 kDa and a
slightly basic pI of 7.7 (The Institute for Genomic Research).
The protein is predicted to be cytoplasmic since no signal sequence is
found. Nudix hydrolases are known to be 17-20 kDa globular monomeric
proteins (30).
Enzyme Activity and Substrate Specificity--
The H. pylori
nudA gene was cloned in pET32a (+), expressed in E. coli strain HMS174(DE3), and purified using the His tag and
nickel-nitrilotriacetic acid resin (Fig.
1). Expression from a pET32a (+) vector
resulted in a protein fused to an N-terminal thioredoxin (Trx) tag in
addition to the His tag, which stabilized the protein and increased the
yield so that ~10 mg of pure soluble protein was obtained. Substrate
specificity of NudA was determined by HPLC analysis. The
relative substrate specificity and reaction products are presented in
Table I. The NudA enzyme showed activity on several polyphosphate substrates, and the preferred substrate was
diadenosine tetraphosphate, Ap4A, as for the NudA
orthologues in B. bacilliformis, Caenorhabditis
elegans, and Lupinus angustifolius. The substrates were
all cleaved asymmetrically, and in the case of Ap4A, the
reaction products were AMP and ATP (Fig.
2). In the same reaction, a small amount
of the ATP was further hydrolyzed to ADP. NudA was not active on the
substrates NADH and ADP-ribose.
Kinetic Properties of NudA--
The Km of NudA
was 80 µM on the preferred substrate Ap4A,
calculated as the mean of three independent experiments using a
substrate concentration ranging from 10 to 300 µM. NudA hydrolyzes 0.3 µmol of Ap4A min Analyses of NudA Involvement in Stress Responses and in Hydrolysis
of DNA Damaging Components--
Since H. pylori appears to
have only one dinucleoside polyphosphate hydrolase, this bacterium
provides a useful system to examine the biological role of this enzyme.
A mutant of the H. pylori Nudix hydrolase was constructed by
insertion of a kanamycin resistance cassette into the nudA
gene. The cassette was inserted upstream of the active site (Nudix
box), thereby ensuring that all enzymatic activity was lost in this
mutant. To test the hypothesis that NudA is a functional orthologue of
E. coli MutT, we performed a measurement of the mutation
rates using the frequency of rifampicin resistance as an overall
indicator of the bacterial spontaneous mutation formation (29). Our
results showed that there was no difference in the spontaneous mutation
frequency of the mutant and wild-type strains. This result suggested
that NudA does not hydrolyze oxidized deoxynucleotide triphosphates and
that it is not involved in preventing incorporation of mutagenic
compounds such as 8-oxo-dGTP that may result in base pair
substitutions. An alternative possibility is that the NudA protein is
involved in protection against oxidative stress and heat shock. The
mutant strain and the corresponding wild-type strain were compared in growth experiments with and without oxidative stress (hydrogen peroxide
exposure). The nudA mutant was 2-7-fold more sensitive than
the wild type after 1-2 h of exposure of 100 and 300 µM
H2O2 (Fig. 3). In
the experiment in which the strains were exposed to heat (55 °C),
the mutant as well as the wild type died at the same rate, and starting
with 108 cells/ml, the colony-forming units decreased with
approximately four logs after 13 min.
Constitutive Expression of NudA at Different Growth Phases and
during Oxidative Stress--
Protein expression of NudA was analyzed
by Western blot in 10 different H. pylori strains grown on
GC plates, including J99 and 26695. All strains produced the protein in
similar amounts (Fig. 4A). The
size of the protein varied slightly, which could not be confirmed by
DNA sequencing, suggesting post-translational modifications, as has
been seen for many other H. pylori proteins (32).
Western blot analyses were also used to determine the expression level
of the protein in strain 2808 under different growth phases and during
stress (Fig. 4, B and C). The protein was
constitutively expressed throughout growth to stationary phase. In
addition, the protein level was similar in the coccoid form of the
bacteria. The expression of NudA did not change at any of the tested
stress conditions: incubation with 100 and 300 µM
H2O2 for 2 h, heat shock at 42 °C for
10 min or 30 min and at 55 °C for 2 min or 13 min.
Several Nudix enzymes from different organisms, belonging to the
dinucleoside polyphosphate hydrolase subgroup, are characterized, and
the Nudix box sequences of these enzymes have been compared (2).
Interestingly, these enzymes differ in substrate specificity and
catalytic capacity (Table II). The
preferred substrate for H. pylori NudA is Ap4A,
but this Nudix enzyme hydrolyzes a number of nucleoside polyphosphates,
as do the orthologues. Compared with the orthologues, the
purified H. pylori NudA seems to be less efficient in
hydrolyzing the dinucleoside polyphosphates. This could be due to
incorrect folding of the purified protein since the solubility was low,
or the fact that important post-translational modifications are absent
when the protein is expressed in E. coli. Another
explanation could be that the H. pylori enzyme is
intrinsically less efficient when compared with Nudix enzymes in other
species.
1 and a Km of 80 µM, resulting in an asymmetrical cleavage of the molecule
into ATP and AMP. To study the biological role of this enzyme in
H. pylori, an insertion mutant was constructed. There was a
2-7-fold decrease in survival of the mutant as compared with the wild
type after hydrogen peroxide exposure but no difference in survival
after heat shock or in spontaneous mutation frequency. Western blot
analyses revealed that NudA is constitutively expressed in H. pylori at different growth stages and during stress, which would
indicate that this protein has a housekeeping function. Given that
H. pylori is a diverse species and that all the H. pylori strains tested in this study harbor the nudA
gene and show protein expression, we consider NudA to be an
important enzyme in this bacterium.
INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
and HMS174(DE3) were grown at
37 °C on Luria agar plates containing the appropriate antibiotics
for selection of plasmids.
, and subcloned into HMS174(DE3) for protein
expression. The HMS174(DE3) strain containing the nudA
construct was grown at 37 °C in 1.6 liters of LB medium with 50 µg/ml ampicillin to an A600 of 0.5. Isopropyl-
-D-thiogalactopyranoside was added to a
final concentration of 0.4 mM to induce protein expression.
Four h after isopropyl-
-D-thiogalactopyranoside addition, cells were harvested by centrifugation, and the pellet was
then resuspended in 15 ml of lysis buffer (50 mM
NaH2PO4, 300 mM NaCl, 10 mM imidazole, 0.1% N-laurylsarcosine, pH 8.0) and disrupted by three passages through a French press at 140 kilopascals. Bacterial cell debris was removed by centrifugation at
39,000 × g for 20 min. Protein purification made use
of the His tag present on the expressed protein and was performed
according the manufacturer's recommendations using a
nickel-nitrilotriacetic acid matrix (Qiagen, Hilden, Germany). After
purification, the protein was immediately dialyzed twice with 1 liter
of DL1 buffer (50 mM Tris-HCl, pH 8.0, 200 mM
NaCl, 10 mM MgCl2, 2 mM
dithiothreitol, 10% glycerol) and once with 200 ml of DL2 buffer (as
DL1 but 50% glycerol). The concentration of the purified protein was
determined using the Bio-Rad protein assay (Bio-Rad), and H. pylori NudA was stored at
20 °C.
20 °C. The samples were
thawed and immediately used in HPLC analysis in which 100 µl of
sample was applied to a 1-ml Resource Q column (Amersham Biosciences)
at a flow rate of 2 ml/min in 35 mM
NH4HCO3, pH 9.6. The products were eluted and
separated by an 18-min gradient elution from 5 to 100% with 0.7 M NH4HCO3. The proportion of
hydrolyzed substrate was calculated by dividing the peak areas from
samples lacking enzyme with the samples containing enzyme. Each value
represents the mean of two individual analyses with an experimental
difference of 7% or less. The reaction products were determined using
the controls AMP, ADP, and ATP to which the retention times of the
sample products were compared.
RESULTS
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
View larger version (93K):
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Fig. 1.
Expression and purification
of the H. pylori Nudix hydrolase. A 12%
polyacrylamide gel containing 1% SDS stained with Coomassie Brilliant
Blue included the following samples: crude extract of non-induced
(NI) and induced (I) HMS174(DE3) cells containing
pET32a (+) with the inserted H. pylori nudA gene, 6 µg of
purified protein (P), full-length Rainbow protein size
marker (M) (Amersham Biosciences).
Substrate specificity of H. pylori Nudix hydrolase NudA
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Fig. 2.
NudA hydrolyzes
Ap4A asymmetrically. The results of HPLC
analysis of Ap4A incubated with the H. pylori
Nudix hydrolase NudA (solid line) and control without the
enzyme (broken line) are shown. AU, arbitrary
units.
1
mg
1 enzyme corresponding to a kcat
value of 0.15 s
1. The enzyme has a broad pH optimum
ranging from pH 7.0 to 9.0 and a need of divalent metal ions for
activity, e.g. Mg2+, as is the case of other
Nudix hydrolases (31). To rule out the possibility of reduced
activity due to the tags (Trx, S-protein, and His), the enzyme activity
of NudA was compared before and after enterokinase digestion that
cleaves off the tags. Presence of the tags did not affect the enzyme
activity on Ap4A.
View larger version (13K):
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Fig. 3.
Survival of the NudA mutant is lower compared
with the wild-type strain after hydrogen peroxide exposure.
Lines marked with filled symbols represent the
wild-type strain 2808, and lines marked with open
symbols represent the 2808nudA::Km mutant.
Squares represent exposure of 100 µM
H2O2, and triangles represent
exposure of 300 µM. Each time point is calculated as the
mean value of five individual experiments for each strain.
CFU, colony-forming units.
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Fig. 4.
Expression of NudA in H. pylori.
As shown in A, Western blot of the NudA protein from
different H. pylori strains revealed a difference in protein
size. Lane 1 = 26695, lane 2 = J99,
lane 3 = 2808, lane 4 = 67:21,
lane 5 = Hp1, lane 6 = CAG7:8,
lane 7 = Ca52, lane 8 = Ca73,
lane 9 = Ca34, and lane 10 = DU52:2.
B, Western blot analysis showing constitutive expression of
NudA in H. pylori strain 2808 at different growth phases
measured at A600. Lane 1 = 0.2, lane 2 = 0.5, lane 3 = 0.9, lane
4 = 1.3, lane 5 = coccoid from 40-day-old
culture stored in phosphate-buffered saline at 4 °C, and lane
6 = 0.9 of the 2808nudA::Km mutant.
C, H. pylori strain 2808 exposed to different
stress conditions. Lane 1 = no stress, lane
2 = 100 µM H2O2 for
2 h, lane 3 = 300 µM
H2O2 for 2 h, lane 4 = 55 °C for 3 min, lane 5 = 55 °C for 13 min,
lane 6 = 42 °C for 10 min, and lane
7 = 42 °C for 30 min.
DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
Comparison of the kinetic parameters of dinucleoside polyphosphate
hydrolases from different organisms
Ap4A binds the heat shock and oxidative stress response proteins DnaK, GroEL, E89, C45, and C40 (33). To determine whether NudA is also involved in oxidative stress, we used H2O2 challenge and compared the survival of the mutant and the wild type. A 2-7-fold difference was found, and thus the H. pylori NudA seems to be involved in oxidative stress response, possibly through degradation of stress-induced Ap4A.
Ap4A has been detected previously in vitro as a byproduct of aminoacyl-tRNA synthetases and is suggested to act as a metabolic alarmone (34). The constitutive expression of the protein in H. pylori during different growth phases as well as in coccoid forms or after hydrogen peroxide challenge and heat shock indicates that this protein has a housekeeping function rather than being involved in stress responses. Thus, it is not clear whether ApnA (n = 3-6) are signaling molecules or toxic byproducts from metabolic processes in H. pylori.
The lack of increase in spontaneous mutation frequency in the strain with an inactivated nudA gene suggests that NudA is not involved in hydrolyzing mutagenic deoxynucleoside triphosphates. Another indication that NudA and MutT are not functional orthologues is the fact that they use different substrates. In a recent study, it was shown that the E. coli MutT protein is active on dGTP and 8-oxo-dGTP but not on Ap3A, ADP-ribose, or FAD (1).
Recently, it was discovered that a putative H. pylori protein NudB (HP0507), containing 5 of the 9 most important amino acids of a Nudix box, is involved in colonization of suckling mice (35). An enzyme activity screen was performed to determine whether this protein had the same substrate specificity as NudA. The purified NudB protein was active on NADH and ADP-ribose but not on any of the dinucleoside polyphosphates tested for NudA.2 Therefore, we conclude that there is no cross-activity between these two proteins.
To study the possible involvement of NudA in invasion, a gentamicin protection assay was used to compare the invasion frequency of the NudA mutant with the wild type in AGS cells (36). No quantifiable differences were found with this method (data not shown). Therefore, it is not clear whether the NudA protein is involved in H. pylori invasion of gastric epithelial cells. However, considering the low frequency of invasion of H. pylori, the gentamicin protection assay might not be sensitive enough to monitor differences in invasion capacity since complete killing of extracellular bacteria is rarely obtained (20).
H. pylori exhibits high genetic diversity, as shown by a
whole genome microarray analysis of 15 strains, in which 22% of the genes were considered dispensable in one or more strains (37). In
addition, about 7% of the genes differ between the two fully sequenced
strains 26695 and J99 (23). Taking into account that all the 70 clinical isolates of H. pylori tested harbor this gene, as
well as the fact that 10 out of 10 of the examined strains showed
protein expression, we consider NudA to be important but not essential
for this bacterium. In conclusion, this study demonstrates that
the NudA protein in H. pylori is an ubiquitous and
constitutively expressed dinucleoside polyphosphate hydrolase
preferably hydrolyzing Ap4A.
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ACKNOWLEDGEMENTS |
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We thank Essam Refai and Mark Clemens for valuable technical advice and Susanne Nilsson for technical assistance.
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FOOTNOTES |
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* This work was supported by the Swedish Institute for Infectious Disease Control and by grants from the Swedish Foundation for Strategic Research, the Swedish Research Council, and the Swedish Cancer Society.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The on-line version of this article (available at
http://www.jbc.org) contains supplementary data showing the sequence
alignments of nudA from different H. pylori strains.
** To whom correspondence should be addressed. Tel.: 46-8-4572415; Fax: 46-8-301797; E-mail: lars.engstrand@smi.ki.se.
Published, JBC Papers in Press, January 27, 2003, DOI 10.1074/jbc.M212542200
2 A. Lundin, C. Nilsson, M. Gerhard, D. I. Andersson, M. Krabbe, and L. Engstrand, unpublished data.
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ABBREVIATIONS |
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The abbreviations used are: Ap4A, adenosine (5')-tetrahospho-(5')-adenosine; Ap3A, adenosine (5')-triphospho-(5')-adenosine; Ap5A, adenosine (5')-pentaphospho-(5')-adenosine; Ap6A, adenosine (5')-hexaphospho-(5')-adenosine; Gp4G, guanosine (5')-tetraphospho-(5')-guanosine; Gp5G, guanosine (5')-pentaphospho-(5')-guanosine; HPLC, high performance liquid chromatography.
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