From the Center for Molecular Cardiology, the
Department of Pharmacology, the
** Circulatory Physiology Division, Department of Medicine,
Columbia University College of Physicians and Surgeons, New York, New
York 10032, the ¶ Medical Biotechnology Center, University of
Maryland Biotechnology Institute, Department of Physiology, School of
Medicine, University of Maryland, Baltimore, Maryland 21201,
Physiopathologie Cardiovasculaire, INSERM, Montpellier
34295, France, and the § Institute of Molecular
Physiology and Genetics, Slovak Academy of Science, Bratislava 83334, Slovak Republic
Received for publication, July 13, 2002
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ABSTRACT |
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The cardiac ryanodine receptor/calcium release
channel (RyR2) on the sarcoplasmic reticulum (SR) comprises a
macromolecular complex that includes a kinase and two phosphatases that
are bound to the channel via targeting proteins. We previously found
that the RyR2 is protein kinase A (PKA)-hyperphosphorylated in
end-stage human heart failure. Because heart failure is a progressive
disease that often evolves from hypertrophy, we analyzed the RyR2
macromolecular complex in several animal models of cardiomyopathy that
lead to heart failure, including hypertrophy, and at different stages of disease progression. We now show that RyR2 is
PKA-hyperphosphorylated in diverse models of heart failure and that the
degree of RyR2 PKA phosphorylation correlates with the degree of
cardiac dysfunction. Interestingly, we show that RyR2 PKA
hyperphosphorylation can be lost during perfusion of isolated
hearts due to the activity of the endogenous phosphatases in the RyR2
macromolecular complex. Moreover, infusion of isoproterenol resulted in
PKA phosphorylation of RyR2 in rat, indicating that systemic
catecholamines can activate phosphorylation of RyR2 in
vivo. These studies extend our previous analyses of the RyR2
macromolecular complex, show that both the kinase and phosphatase
activities in the macromolecular complex are regulated physiologically
in vivo, and suggest that RyR2 PKA hyperphosphorylation is
likely a general feature of heart failure.
During the "fight or flight" response, sympathetic nervous
system activation modulates the signaling pathway that controls excitation-contraction (EC)1
coupling in the heart resulting in increased cardiac output required to
meet stress-induced increases in metabolic demands. EC coupling in
heart muscle converts electrical signals to mechanical ones via
elevation of cytosolic calcium (Ca2+), which triggers
contraction. The major components that regulate cardiac EC coupling are
all modulated by activation of the sympathetic nervous system via
phosphorylation by adenosine 3',5'-monophosphate (cAMP)-dependent protein kinase (PKA). These include: 1)
the triggers for EC coupling, predominantly Ca2+ influx
through the voltage-gated Ca2+ channel (dihydropyridine
receptor) on the plasma membrane and possibly Ca2+ influx
via the Na+-Ca2+ exchanger; 2) the SR
Ca2+ release channel or type 2 ryanodine receptor (RyR2);
and 3) the SR Ca2+ reuptake pump (SERCA2a) and its
regulatory protein phospholamban (PLB). Although the roles of the
sarcolemmal Ca2+ channel and PLB phosphorylation are well
established, the physiological significance of RyR2 PKA phosphorylation
is not yet fully understood.
RyR2 is one of the largest known ion channels. Each RyR2 has an
enormous cytoplasmic domain that serves as a scaffold for regulator
proteins that modulate the channel's activity (1). The RyR2
cytoplasmic scaffold domain contains highly conserved leucine/isoleucine zippers that bind to leucine/isoleucine zippers present in the adaptor/targeting proteins for kinases (PKA) and phosphatases (PP1 and PP2A) that regulate RyR channel function (1). In
1989 Marks et al. (2) discovered a 12-kDa protein that
co-purifies with RyR1 and was later shown to be FKBP12, the cytosolic
binding protein for the immunosuppressant drugs FK506 and rapamycin
(3-5). A 12.6-kDa isoform of FKBP12 (FKBP12.6) is tightly associated
with RyR2 and modulates its function, possibly by enhancing
cooperativity among its four subunits (4, 6-8). Four FKBP molecules
are part of each RyR channel (9). So, in fact, RyR2 is a macromolecular
complex containing kinases, phosphatases, and FKBP.
The sympathetic nervous system is activated in response to stress,
resulting in increased levels of local and circulating catecholamines
that activate PKA hyperphosphorylation of RyR2 also decreases coupled gating between
RyR2 (26, 27) due to dissociation of FKBP12.6 from the channel. Reduced
coupled gating may lead to incomplete closure of RyR2 following
activation (14, 15) that would tend to decrease SR Ca2+
content. Administration of oral Here we analyze the RyR2 PKA phosphorylation and the RyR2
macromolecular complex in several models of heart failure, including hypertrophy that leads to heart failure, and at different stages of the
disease. We now show that, in isolated perfused rodent hearts,
inhibition of endogenous phosphatase activity results in a significant
increase in PKA phosphorylation of RyR2 in response to isoproterenol,
indicating that phosphatases are indeed active in the RyR2
macromolecular complex in intact heart. Moreover, infusion of
isoproterenol resulted in PKA phosphorylation of RyR2 in rat,
indicating that systemic catecholamines can activate phosphorylation of
RyR2 in vivo. Using a rat model of myocardial
infarction-induced heart failure, we demonstrate that RyR2 is
PKA-hyperphosphorylated while in the same hearts phospholamban is
PKA-hypophosphorylated. Using this model we further show that the RyR2
macromolecular complex is depleted of FKBP12.6 and PP1 and PP2A. RyR2
PKA hyperphosphorylation was also demonstrated in a murine model of
cardiac-specific Immunoprecipitation and Back-phosphorylation of Ryanodine
Receptor--
Heart homogenates were prepared by homogenizing ~1.0 g
of cardiac tissue in 1.0 ml of a buffer containing 50 mM
Tris-HCl (pH 7.4), 200 mM NaCl, 20 mM NaF, 1.0 mM Na3VO4, 1.0 mM
dithiothreitol, and protease inhibitors. Samples were centrifuged at
3000 × g for 10 min. After determining the protein
concentration, the supernatants were aliquoted and stored at Immunoblot--
Proteins were size-fractionated on 6% SDS-PAGE
for RyR, 12% SDS-PAGE for RII, PKA, PP1, and PP2A, and 15% SDS-PAGE
for phospholamban and FKBP12.6. Immunoblots were developed using
Anti-RyR (Affinity Bioreagents, Golden, CO, 1:3000 dilution in 5%
milk-TBS-Tween), a phosphospecific phospholamban antibody (Cyclacel
Limited, Dundee, Scotland, 1:5000 dilution in 5% milk-TBS-Tween),
anti-RII (1:1000), anti-PKA catalytic subunit (1:1000), anti-PP1
(1:1000), anti-PP2A (Transduction Laboratories), and anti-FKBP
(1:1000).
Isoproterenol Infusion of Rats--
Rats were anesthetized with
a mixture of intraperitoneal 100 mg/kg ketamine and 10 mg/kg xylazine.
Vessel cannulation was performed using a 2-cm longitudinal incision
made below the mandible to the thoracic inlet. The left internal
jugular vein was isolated and cannulated with an Intramedic PE-10
polyethylene catheter. Isoproterenol hydrochloride (0.2 mg/ml) was
infused through the venous catheter at a rate ranging from 0.1 to 1.0 ml/min until the mean arterial pressure decreased to 60% of baseline.
Rat Infarct Model--
Male Wistar rats (180-200 g) were used
to produce myocardial infarction as described previously (29). Briefly,
rats were anesthetized with a mixture of 150 mg/kg intraperitoneal
ketamine and 15 mg/kg chlorpromazine. Respiration was maintained with a ventilation assist device and an endotracheal tube (3 ml air/60 strokes
per minute). After median-left thoracotomy and pericardium opening, the
left main coronary artery was occluded with a 7-0 silk suture at the
most proximal point below the left atria appendage. Sham-operated rats
were treated the same way but without coronary artery ligation.
Animals were euthanized for experiments at 6-month post-ligation.
Before they were euthanized, animals underwent hemodynamic measurements
or echocardiography.
Cardiomyocyte Isolation and Calcium Spark
Measurements--
Ventricular cardiac myocytes were isolated from
control and PMI myocytes by standard enzymatic (collagenase Worthington
type II, 1 mg/ml) methods. After isolation, cells from the left
ventricular free wall (around the infarction) were stored in DEM media
(1.8 mM CaCl2). The cells were loaded with the
fluorescence Ca2+ dye Fluo-3 through the patch-clamp
pipette. Data are shown as the ratio of measured fluorescence
(F) over fluorescence min (Fo). All
images were acquired with a Bio-Rad MRC 600 confocal microscope fitted
with an argon laser and processed with Bio-Rad SOM, COMOS, and IDL
(Research Imaging Systems, Boulder, CO) software. Ca2+
sparks were evoked by depolarizations to voltages close to the activation threshold of ICa using an Axopatch
200A patch-clamp amplifier and recorded using pClamp 6.01 (Axon
Instruments). Holding potential was
Cells were superfused with an external solution containing (in
mM) NaCl 135; MgCl2 1; CsCl 20; glucose 10;
HEPES 10; CaCl2 1.8 (pH 7.4). When isoproterenol was used,
10 µM tetrodotoxin was used to block
Na+ channels. Patch pipettes were filled with (in
mM) CsCl 130; fluo-3 0.05; HEPES 10; MgCl2
0.33; triethanolamine-Cl 20; Mg-ATP 4 (pH 7.2). Temperature was
maintained at 35-37 °C throughout each experiment.
Perfusion of Normal Rat Hearts--
Briefly, male Sprague-Dawley
rats weighing between 250 and 300 g were euthanized by lethal
injection of pentobarbital sodium (100 mg/kg). The hearts were rapidly
removed and perfused via the Langendorff method with
Ca2+-free modified Tyrode's solution containing
(mM) NaCl 137, KCl 5, HEPES 20, MgCl2 0.5, glucose 15, and NaH2PO4 1 with pH 7.4 at
37 °C for 5 min until the blood was washed out. Hearts were then
perfused with 1 µM isoproterenol for 15 min or identical procedure in the complete absence of isoproterenol. The same procedure was repeated in the presence of 1 mM NaF during the whole
perfusion (20 min) with or without isoproterenol (1 µM).
Following perfusion, the heart ventricles were removed and quickly
frozen (liquid nitrogen) until further use.
Perfusion of Hypertrophic Hearts--
Dahl salt-sensitive (SS)
and Dahl salt-resistant (control, Dahl SR/Jr) rats were fed an 8% high
salt diet from 6 weeks of age as described previously (30). SS Dahl
rats develop severe hypertension and concentric compensated hypertrophy
over a period of weeks when fed a high salt diet, the SR/Jr strain do
not develop hypertension or cardiac hypertrophy. After 8 weeks on diet
the rats were sacrificed, and the hypertrophic hearts were perfused using the procedure described above with or without 1 mM
NaF in the perfusate as indicated, except that no isoproterenol was
added to the perfusion solution.
Single Channel Recordings--
Single channel recordings of RyR2
were performed and analyzed under voltage-clamp conditions as described
previously (16). We used planar lipid bilayers consisting of 3:1
phosphatidylethanolamine:phosphatidylserine (Avanti Polar Lipids, 20 mg/ml) formed across a 0.15-mm aperture in a polystyrene cup (Warner
Instruments). SR membrane vesicles were added to the cis
(cytoplasmic) side of the membrane and induced to fuse with the lipid
bilayer by increasing KCl concentration to 800 mM. After
incorporation of vesicles with channels to bilayer, the KCl gradient
across the bilayer was eliminated by perfusion of the cis
chamber with cis solution. Solutions used for channel analysis were: trans solution, 250 mM Hepes, 53 mM Ca(OH)2, and 50 mM KCl (pH 7.4);
cis solution, 250 mM HEPES, 125 mM
Tris, 50 mM KCl, 1 mM EGTA, 0.5 mM
CaCl2 (pH 7.4). Free [Ca2+] (cis)
150 nM was calculated using CHELATOR software (31). The
trans chamber of the lipid bilayer was connected to the
head-stage input of an Axopatch 200A amplifier (Axon Instruments) using
an Ag/AgCl electrode and an agarose/KCl bridge. The cis side
was held at ground with a similar electrode. The single-channel
currents were filtered at 1 kHz with a four-pole Bessel filter (Warner Instruments) and digitized with a 4-kHz sampling frequency using a
DigiData 1200 interface (Axon Instruments) with AxoScope1 software (Axon Instruments). At the conclusion of each experiment, ryanodine or
ruthenium red were applied to confirm the identity of channels as RyR2.
pClamp 6.0.3 software (Axon Instruments) was used for analyzing channel
records. Open probability (Po) and open and closed dwell times were identified using a 50% threshold analysis from
at least 2 min of continual records. To find mean dwell times ( Given the strong evidence showing PKA hyperphosphorylation of RyR2
in heart failure as noted above (16, 28), it was initially hard to
reconcile the findings of Gomez et al. (30) who reported that RyR2 function was normal in preparations obtained from animals in
heart failure. To resolve this paradox, we explored the hypothesis that
it may be necessary to prevent dephosphorylation of RyR2 by
phosphatases to observe the PKA phosphorylated state of RyR2 in
isolated cardiomyocytes. Fig.
1A shows the result of an
experiment that investigates this process. Phosphorylation of RyR2 and
phospholamban (PLB) were examined. Langendorff perfusion of a control
heart with isoproterenol (1 µM) only produced PKA
phosphorylation of RyR2, if the weak phosphatase inhibitor NaF (1 mM) was present. NaF perfusion alone did not produce RyR2
phosphorylation. In contrast, PLB was phosphorylated by isoproterenol
in the presence or absence of NaF. This finding suggests that studies
performed on isolated cardiomyocytes that are harvested following a
similar Langendorff perfusion do not accurately represent the
phosphorylation status of RyR2 in the intact heart due to continuing
activity of the bound phosphatases during the perfusion. In contrast,
phospholamban, a small protein that does not have phosphatases and
kinases targeted directly to it, is regulated differently from RyR2
with respect to phosphorylation and dephosphorylation in the isolated
perfused heart.
INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
-adrenergic receptors (
-AR), elevate intracellular
cAMP, and activate PKA. PKA phosphorylation modulates RyR2 function
both in vitro and in vivo (1, 10-20). Channels from failing hearts have been shown to be PKA-hyperphosphorylated leading to dissociation of the FKBP12.6 and altered RyR2 channel function (14-16, 21). Thus, maladaptation of a classic physiological pathway ("fight or flight" that has never been subjected to
evolutionary pressure, because heart failure is a new disease of the
last century or two) plays an important role in the pathophysiology of
heart failure, which is a chronic hyperadrenergic state (15, 21). PKA
hyperphosphorylation of RyR2 in failing hearts shifts the sensitivity
of RyR2 to Ca2+ to the left (16) resulting in "leaky"
channels that can deplete SR Ca2+ stores and contribute to
reduced Ca2+ transients that may impair contractility. If
conditions lead to transient SR Ca2+ overload, then the SR
Ca2+ leak during repolarization may contribute to "early
after depolarizations" (EADs) and during diastole initiate "delayed
after depolarizations" (DADs) by activating "transient inward
currents" (22). EADs and DADs can trigger fatal cardiac arrhythmias
(e.g. ventricular tachycardia) (23-25). How
Ca2+ signaling pathways interact to produce alterations in
the [Ca2+]i transient observed in heart
failure is not well understood. Here we examine how RyR2 PKA
phosphorylation is regulated and how this form of regulation may be
linked to changes in RyR2 function.
-AR blockers reverses the PKA hyperphosphorylation of RyR2, restores normal stoichiometry of the RyR2
macromolecular complex, and restores normal single channel function in
a canine model of heart failure (28).
2-AR overexpression confirming that the
hyperphosphorylation of RyR2 is due to activated PKA. Moreover, a
murine cardiomyopathy model in which cardiac dysfunction is associated
with expression of collagenase in the heart also results in PKA
hyperphosphorylation of RyR2 that is progressive over time and
correlates with the degree of cardiac dysfunction. These studies extend
our previous studies on the RyR2 macromolecular complex and show that
the both the kinase and phosphatase activities in the macromolecular
complex can be regulated physiologically in vivo.
MATERIALS AND METHODS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
80 °C
until use. RyR2 was immunoprecipitated from heart samples by incubating
500 µg of homogenate with anti-RyR antibody in 0.5 ml of a modified
radioimmunoprecipitation assay buffer (50 mM Tris-HCl (pH
7.4), 0.9% NaCl, 5.0 mM NaF, 1.0 mM Na3VO4, 0.25% Triton X-100, and protease
inhibitors) overnight at 4 °C. Protein A-Sepharose beads were added,
incubated at 4 °C for 1 h, washed with 1× phosphorylation
buffer (8 mM MgCl2, 10 mM EGTA, and
50 mM Tris/PIPES, pH 6.8), and resuspended in 10 µl of a
1.5× phosphorylation buffer containing either PKA catalytic subunit
(Sigma Chemical Co., St. Louis, MO) or PKA plus a PKA inhibitor
(PKI5-24, 500 nM, Calbiochem, San Diego, CA). Back-phosphorylation of immunoprecipitated RyR2 was initiated with 33 µM Mg-ATP containing 10% [
-32P]ATP
(PerkinElmer Life Sciences, Boston, MA) and terminated after 8 min at
room temperature with 5 µl of stop solution (4% SDS and 0.25 M dithiothreitol). Samples were heated to 95 °C,
size-fractionated on 6% SDS-PAGE, and RyR2 radioactivity was
quantified using a PhosphorImager and ImageQuaNT software (Amersham
Biosciences, Piscataway, NJ). Nonspecific phosphorylation (not
inhibited by PKA inhibitor) was subtracted, and the resulting value was
divided by the amount of RyR2 protein and expressed as the inverse of the PKA-dependent [
-32P]ATP signal.
80 mV, then a ramp (500 ms) to
45 mV was applied to inactivate Na+ channels, and 200-ms
depolarizations were applied in 2-mV steps until no individual sparks
but a global [Ca2+]i transient was evoked. Series
resistance was electronically compensated to 40-60%.
i) and their proportions (area under the curve for each
exponential distribution), the dwell time distributions were fitted
using the Levenberg-Marquardt least-squares method with SigmaPlot 4.00 software (Jandel Scientific). Distributions were generated by pooling
data for individual channels (n > 3) to get precise
fitted parameters. The F test was employed for statistical
comparisons of different kinetics models. Events shorter than 500 µs
were excluded from analyses. Results are presented as mean ± S.D.; Student's t test or ANOVA (where indicated) was used
for statistical analyses. The unpaired t test or Welch
t test were used for statistical analysis. Mean dwell times
are presented as the value of the fitted coefficient ± S.E. (S.E.
represents the uncertainty in the estimates of the fitted coefficient).
A p value less than 0.05 was considered statistically significant.
RESULTS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
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Fig. 1.
Isoproterenol induces PKA phosphorylation of
RyR2 and phospholamban in intact hearts. Rat hearts were perfused
via the Langendorff method with Ca2+-free modified
Tyrode's solution containing isoproterenol 1 µM
(Iso), the phosphatase inhibitor sodium fluoride 1 mM (NaF), NaF plus Iso, or buffer. In
A: The upper panel shows an autoradiogram of
back-phosphorylation of immunoprecipitated RyR2 with PKA and
[ -32P]ATP with or without PKA inhibitor
(PKI). The middle panel shows an immunoblot of
immunoprecipitated RyR2 to confirm that equivalent amounts of RyR2
protein were present in each back-phosphorylation assay. The
lower panel shows an immunoblot of heart lysate with
anti-phospholamban antibody that detects the phosphorylation of serine
16 (PS-16). The bar graph shows the relative PKA
phosphorylation of RyR2 and phospholamban in the various treatment
groups. In B: The upper panels are immunoblots of
immunoprecipitations using anti-RyR antibody (5029) showing levels of
components of the RyR2 macromolecular complex including RyR2, the PKA
regulatory subunit RII, PKA catalytic subunit, PP1, PP2A, and FKBP12.6.
The accompanying bar graph shows the relative amounts of
each of the components of the RyR2 macromolecular complex in response
to control, Iso, NaF, and Iso plus NaF. C, RyR2 PKA
phosphorylation was measured following infusion of isoproterenol via
the jugular vein in rats (n = 2). D,
upper panels are immunoblots of immunoprecipitations using
anti-RyR antibody (5029) showing levels of components of the RyR2
macromolecular complex, including RyR2, the PKA regulatory subunit RII,
PKA catalytic subunit, PP1, PP2A, and FKBP12.6. The accompanying
bar graph shows the relative amounts of each of the
components of the RyR2 macromolecular complex in response to
isoproterenol infusion. E, immunoblot showing: lane
1, FKBP12.6 and FKBP12 both detected in human cardiac homogenate
(50 µg) using an anti-FKBP antibody that recognizes both proteins;
lane 2, an immunoprecipitation from the same cardiac
homogenate (300 µg) using an anti-RyR-5029 antibody showing that only
FKBP12.6 co-immunoprecipitates with RyR2 in heart. The top
half of the gel was immunoblotted with anti-RyR antibody; the
bottom half was immunoblotted with anti-FKBP12 antibody.
RyR2 is not seen in lane 1 because insufficient cardiac
homogenate was added. *, p < 0.01 for the indicated
comparison.
PKA phosphorylation of RyR2 leads to dissociation of FKBP12.6 from the channel resulting in altered RyR2 function. Therefore, it is important to test what, if any, changes in the macromolecular complex take place following the treatment of Langendorff-perfused control hearts with isoproterenol as shown in Fig. 1A. To assess the effect of acute PKA phosphorylation of RyR2 in an intact heart on the RyR2 macromolecular complex we immunoprecipitated the complex from the perfused rat hearts with an anti-RyR2 antibody and immunoblotted for components of the RyR2 macromolecular complex (Fig. 1B). All of the components of the macromolecular complex are shown to remain at the same levels as in control (non-isoproterenol-treated) except FKBP12.6. FKBP12.6 was reduced by about 40% following perfusion with isoproterenol and NaF, but there was no significant reduction following perfusion by either isoproterenol or NaF alone. These findings are consistent with the depletion of FKBP12.6 from the RyR2 complex due to the PKA hyperphosphorylation of RyR2 in heart failure (16). The notable difference between the acute PKA phosphorylation of RyR2 shown here and the longer-term PKA phosphorylation of RyR2 in heart failure are the reductions of the phosphatases PP1 and PP2A in heart failure (16) that were not observed following acute PKA phosphorylation due to isoproterenol perfusion (Fig. 1A). This finding suggests that the acute effect of isoproterenol-induced PKA phosphorylation of RyR2 is to deplete the complex of FKBP12.6. The reduction in phosphatase levels in the RyR2 macromolecular complex that we have observed in channels from failing hearts (16) represents a distinct effect of the chronic hyperadrenergic state of heart failure that is not completely recapitulated with acute perfusion with isoproterenol.
An important question that needs to be addressed is whether or not
RyR2s in the heart become PKA-phosphorylated following systemic
infusion of isoproterenol in the intact animal? Infusion of
isoproterenol hydrochloride (0.2 mg/ml) at a rate ranging from 0.1 to
1.0 ml/min was conducted until the mean arterial pressure decreased to
60% of baseline at which point the animals were sacrificed, and the
hearts were excised and immediately flash-frozen so that there was no
time for phosphatases bound to RyR2 to dephosphorylate the channel.
Under these conditions isoproterenol infusion resulted in a significant
increase in PKA phosphorylation of RyR2 (Fig. 1C).
Isoproterenol infusion reduced the amount of FKBP12.6 bound to the RyR2
complex but did not alter levels of PKA, RII, PP1, or PP2A (Fig.
1D). Although both FKBP12 and FKBP12.6 are expressed in the
heart, and FKBP12 is considerably more abundant than FKBP12.6, only
FKBP12.6 co-immunoprecipitated with RyR2 (Fig. 1E). These data provide compelling evidence that -AR activation leads to RyR2
PKA phosphorylation and the dissociation of FKPB12.6.
Investigation of the phosphorylation/dephosphorylation balance
hypothesis to understand PKA hyperphosphorylation of RyR2 in cardiac
hypertrophy and failure requires use of more than one model. To assess
the PKA phosphorylation state of RyR2 and phospholamban in a
hypertrophic cardiomyopathy model, the salt-sensitive (SS) Dahl rat was
used. SS Dahl rats were maintained on a high salt diet for 8 weeks to
produce hypertension and cardiac hypertrophy. Hearts from these animals
were isolated and perfused using the Langendorff method and compared
with hearts from control animals (age-matched strain that remains
normotensive on the same high salt diet, Dahl SR/Jr). Following
Langendorff perfusion, hyperphosphorylation of RyR2 was observed in
Dahl rat hearts in the presence of NaF (1 mM) but not in
its absence and not in control. In contrast, phospholamban was
PKA-phosphorylated whether or not NaF was added to the perfusate (Fig.
2A). These data show that RyR2
and PLB are differentially regulated with respect to PKA
phosphorylation in the severe hypertrophy of the Dahl-hypertensive rat.
Importantly, these data are the first to show that RyR2 can be
PKA-hyperphosphorylated in severe cardiac hypertrophy and that this is
not just a feature found in end-stage heart failure. Moreover, this is
the fist report of a condition in which both RyR2 and PLB are
PKA-hyperphosphorylated in the same cardiomyopathy (in contrast to
heart failure in which RyR2 is PKA-hyperphosphorylated but PLB is
not).
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The RyR2 macromolecular complex was examined using
immunoprecipitation with an anti-RyR2 antibody followed by
immunoblotting for components of the RyR2 macromolecular complex (Fig.
2B). We found a reduction of ~40% in the amount of
FKBP12.6 (p < 0.001) in the RyR2 macromolecular
complex that correlated with the increase in PKA phosphorylation of
RyR2 (Fig. 2A). However, the levels of the phosphatases PP1
and PP2A were not significantly decreased (Fig. 2B)
suggesting that hypertrophic cardiomyopathy-induced PKA phosphorylation
of RyR2 differs from that seen in failing hearts in which we have
previously documented a reduction in phosphatase levels in the RyR2
macromolecular complex (16). In this matter, the Dahl rat more closely
approximates acute AR activation as shown in Fig. 1.
Our studies of human and canine heart failure (16) revealed changes in
the cardiac RyR2 macromolecular complex, including PKA
hyperphosphorylation of RyR2 and dissociation of FKBP12.6 from RyR2.
Here we examined hearts from a rat model in which heart failure
develops 6-month post-myocardial infarction (PMI) produced by ligation
of the left anterior descending coronary artery. At 6 months PMI hearts
were excised (following deep anesthesia) and immediately flash-frozen
in liquid nitrogen. Fig. 3A
shows PKA hyperphosphorylation of RyR2 and PKA
hypophosphorylation of PLB from the same hearts (Fig.
3A) providing additional evidence that RyR2 and PLB are
differentially regulated with respect to PKA-dependent phosphorylation. The proteins of the macromolecular cluster involving RyR2 were also changed as shown in Fig. 3B (16). We found
that FKBP12.6 was depleted from the RyR2 macromolecular complex in the
failing rat hearts and there was a reduction in the levels of PP1 and
PP2A (Fig. 3B), consistent with our previous reports concerning heart failure (16) but distinctive from cardiac hypertrophy (Fig. 2).
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Mice with cardiac-specific expression of the human matrix
metalloproteinase type 1 (MMP-1) develop progressive cardiac
dysfunction over time (32). We measured PKA phosphorylation of RyR2 in
these mice at 6, 12, and 24 months of age. Compared with age-matched WT
littermates the MMP-1 TG mice exhibited progressively increasing PKA
hyperphosphorylation of RyR2 (Fig.
4A) that correlated with the
degree of cardiac dysfunction (32). Interestingly, even at 6 months,
before hemodynamic compromise of the left ventricular function is
detected, there was a trend toward increased PKA phosphorylation of
RyR2 suggesting that this is an early event in the progression to heart
failure. The PKA hyperphosphorylation of RyR2 was associated with a
depletion of FKBP12.6 in the RyR2 macromolecular complex (Fig.
4A).
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Mice with cardiac specific overexpression of the 2-adrenergic
receptor (
2-AR TG) exhibit cardiac dysfunction that progresses with
age and correlates with the degree of cardiac overexpression of the
transgene (33). The overexpression of
2-AR leads to increased
activity of PKA, because a fraction of all
-ARs is activated even in the absence of agonist (33). That such activation occurs in this animal model is supported by the significant PKA hyperphosphorylation of RyR2 in the
2-AR TG mouse hearts (Fig. 4B). We found that RyR2 was PKA-hyperphosphorylated and that
the RyR2 macromolecular complex was partially depleted of FKBP12.6 (Fig. 4B) consistent with the data presented above and those
reported previously for human and canine RyR2 (16, 28). Taken together these data indicate that activation of the
2-AR signaling pathway in
the mouse heart can result in PKA phosphorylation of RyR2 and depletion
of FKBP12.6 from the RyR2 macromolecular complex.
To investigate the gating behavior of the PKA-hyperphosphorylated RyR2,
we examined RyR2 single channel properties in planar lipid bilayers
with low [Mg2+] (to increase Po).
Fig. 5 compared RyR2 gating from RyR2
from WT mouse hearts, MMP-1 transgenic mouse hearts and 2-AR
transgenic mouse hearts. The RyR2 from WT are relatively PKA
hypophosphorylated, and from MMP-1 and
2-AR transgenic mouse
hearts RyR2 are PKA-hyperphosphorylated. FKBP12.6 was significantly
depleted from the PKA-hyperphosphorylated RyR2 macromolecular
complexes. Under control conditions with 150 nM
cis (cytosolic) [Ca2+], the RyR2 channel
activity was lowest in WT with an open probability (Po) of 0.01 ± 0.01 (n = 12). RyR2 channel activity increased with PKA hyperphosphorylation of
RyR2 and FKBP12.6 dissociation. The Po was
0.35 ± 0.03 (n = 11) for MMP-1 RyR2, and
0.15 ± 0.04 (n = 14) for
2-AR RyR2
(p < 0.001 versus WT channels for both MMP-1 and
2-AR channels by ANOVA). ATP, in the absence of
Mg2+ activates the RyR2 and we used the activation by 1 mM ATP in the continued presence of 150 nM
[Ca2+] to further probe the behavior of the PKA
phosphorylated RyR2. The Po of WT was 0.47 ± 0.10 (n = 12), of MMP-1 was 0.55 ± 0.14 (n = 11) and
2-AR was 0.85 ± 0.14 (n = 14, p < 0.001 versus
WT channels for both MMP-1 and
2-AR RyR2 channels by ANOVA). Thus PKA hyperphosphorylation of RyR2 is associated with increased RyR2
Po regardless how the PKA-dependent
phosphorylation was produced. These findings are consistent with the
increase in Po by PKA phosphorylation shown in
RyR2 (19).
|
MMP-1 and 2-AR channels exhibit significantly faster gating kinetics
(shorter open and closed events) compared with WT channels. The dwell
time distributions for WT, MMP-1, and
2-AR channels exhibited three
distinct dwell times (
1 <
2 <
3) corresponding to three different open states and
three different closed states of channels. All three mean open times
(
o) were significantly longer for WT channels compared
with MMP-1 and
2-AR channels (Table
I). In addition,
o2 and
o3 for WT channels were one order of magnitude
longer than the corresponding
o2 and
o3
exhibited by MMP-1 and
2-AR channels. MMP-1 and
2-AR channels
never exhibited open states with mean open times longer than 100 ms; in
contrast, 62% of the WT channel openings were to this long open state
(Table I). MMP-1 and
2-AR channels preferentially occupied the open state with the shortest mean open time (Table I). Analyses of the
closed states (
c) of the WT, MMP-1, and
2-AR channels
revealed that
c1 was significantly shorter for WT
channels in comparison with MMP-1 and
2-AR channels (Table
II), but
c1 represented only 17% of the closings by WT channels.
c2 and
c3 were significantly longer for WT channels than the
corresponding
c for MMP-1 and
2-AR channels. In
addition,
c3 for WT channels was one order of magnitude
larger than for MMP-1 and
2-AR channels, exceeding 100 ms. MMP-1 and
2-AR channels never exhibited this long lasting closed state. MMP-1
and
2-AR channels exhibited equal distributions of each closed
state, whereas 73% of the WT channel closings were to the longest
closed state. Taken together these data are consistent with the
destabilization of the MMP-1 and
2-AR channels due to PKA
phosphorylation-induced dissociation of FKBP12.6 from the channels. In
summary, PKA phosphorylation causes dissociation of FKBP12.6 from the
RyR2 channel. FKBP12.6 stabilizes the RyR2 channel in both the closed
and open state. Thus, when FKBP12.6 is removed from the channel due to
PKA phosphorylation, both the open and closed times are shortened.
|
|
It is well established that isoproterenol response is markedly reduced
in failing hearts (34), yet studies of heart failure tissues have
reported effects of isoproterenol on cardiac contractility that are not
observed in vivo (35). Possible explanations for this
discrepancy are that RyR2 becomes dephosphorylated during the
experiments and that the isoproterenol responses ex vivo or in vitro do not reflect the in vivo situation
with respect to cardiac EC coupling. To test this hypothesis we
examined the effects of isoproterenol on Ca2+ sparks in
cardiomyocytes isolated from post-myocardial infarction (PMI) failing
rat hearts in the absence of NaF. Based on our findings that RyR2 PKA
hyperphosphorylation is lost during isolation of cardiomyocytes, we
would predict that isoproterenol responsiveness would be restored in
PMI cardiomyocytes isolated in the absence of NaF. Following
isoproterenol application in PMI ventricular myocytes, Ca2+
sparks decayed more slowly (increased duration, p < 0.001), had smaller peak levels (decreased
F/Fo, p < 0.001) and
were wider (increased width, p < 0.02) (Fig.
6). These findings are consistent with
reduced coupled gating between individual RyR2, which is a direct
consequence of PKA phosphorylation-induced depletion of FKBP12.6 from
the RyR2 macromolecular complexes (26, 27). In these studies
isoproterenol was applied directly to the cardiomyocytes, so the
problem of dephosphorylation of RyR2 during the isolation of cells was
not an issue. The finding that isoproterenol has effects on
Ca2+ sparks that are consistent with the effects of PKA
phosphorylation of RyR2 as seen in bilayer experiments (16, 19) is in
agreement with our finding that these RyR2 become dephosphorylated
during the perfusion step required for cardiomyocyte isolation. If the RyR2 had remained PKA-hyperphosphorylated, the addition of
isoproterenol would have had no additional affect on RyR2 channels as
they would have been already essentially maximally PKA-phosphorylated.
Although the biochemical assays are performed on flash-frozen hearts,
the isolation of cardiomyocytes involves perfusion of the intact heart with a catecholamine-free solution and the cardiomyocytes are maintained in catecholamine-free culture media until use. During this
time, the RyR2-bound phosphatases dephosphorylate the channel in the
absence of NaF.
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DISCUSSION |
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In this study we show for the first time that acute infusion of isoproterenol causes PKA phosphorylation of RyR2 and depletion of FKBP12.6 from the RyR2 macromolecular complex in vivo. PKA phosphorylation of RyR2 and depletion of FKBP12.6 from the RyR2 macromolecular complex are similar to the effects of RyR2 PKA hyperphosphorylation observed in human heart failure (16). The difference being that acute isoproterenol infusion is a transient effect, and the effects of heart failure generally persist for years. These data show that catecholamine release, as part of the "fight or flight" stress response, can modulate RyR2 function via PKA phosphorylation (Fig. 1).
Although acute administration of isoproterenol depletes the RyR2 macromolecular complex of FKBP12.6, it does not reduce the levels of phosphatases in the complex (Fig. 1), as occurs in the RyR2 complexes during chronic heart failure (16). Significantly, this finding points out a difference between the effects of acute administration of isoproterenol and heart failure, which is a chronic hyperadrenergic state. We found that, in contrast to experiments using flash-frozen heart samples, it was necessary to include the mild phosphatase inhibitor NaF, to preserve RyR2 PKA phosphorylation and FKBP12.6 dissociation following isoproterenol treatment using Langendorff perfusion of intact hearts. In contrast, PLB is also PKA-phosphorylated by the isoproterenol treatment, but its phosphorylation state can be readily observed in the absence of a phosphatase inhibitor.
Thus, two important PKA phosphorylation targets, RyR2 and PLB, are regulated by distinct mechanisms. Because NaF was required to observe the increased PKA phosphorylation of RyR2 but not of PLB in cardiomyocytes isolated from control perfused hearts, our results suggest that the background phosphatase activity is relatively high for RyR2 but not for PLB. This distinction is likely explained by the fact that RyR2s comprise a macromolecular complex in which the phosphatases PP1 and PP2A are targeted directly to the channel (PKA, PP1, and PP2A bind to RyR2 via targeting proteins by virtue of leucine/isoleucine zippers (1)), and there is no evidence that phosphatases are targeted by a similar mechanism directly to PLB. Taken together these data support the concept that the RyR2-macromolecular complex is important in the regulation of local Ca2+ signaling in heart muscle. Moreover, these findings suggest that special care is needed when examining RyR2 PKA phosphorylation and the role of RyR2 PKA phosphorylation in cardiac signaling. For example, when cardiomyocytes are isolated from failing hearts using the widely accepted method of Langendorff perfusion, the RyR2 channels appear to become dephosphorylated.
It is important to stress that accurate measurement of PKA phosphorylation of RyR2 can be obtained without inhibition of phosphatases when cardiac tissue is immediately flash-frozen prior to measuring PKA phosphorylation of RyR2. This is the technique that we have routinely used to examine PKA phosphorylation of RyR2 in failing hearts (16, 28, 36, 37). Phosphatase inhibition is only required when measurements are made following isolation of cells or perfusion of intact hearts, when, in both circumstances, phosphatase activity in the RyR2 macromolecular complex can dephosphorylate the RyR2 channel and prevent accurate assessment of the in vivo level of PKA phosphorylation of RyR2. The finding that isoproterenol infusion in vivo results in PKA phosphorylation of RyR2 (Fig. 1, C and D), whereas perfusion of an intact heart ex vivo with isoproterenol results in only a small increase in PKA phosphorylation of RyR2 unless NaF is included in the perfusate, in which case there is equivalent PKA phosphorylation of RyR2 (Fig. 1, A and C), indicates that the balance of phosphorylation/dephosphorylation favors dephosphorylation under ex vivo conditions. Nevertheless, the significance of these findings are that, under in vivo conditions systemic isoproterenol can induce PKA phosphorylation of RyR2; under ex vivo conditions that are frequently used for experimental purposes it is important to inhibit the endogenous phosphatase activity to PKA phosphorylate RyR2.
We recognize that addition of NaF to the Langendorff perfusate could potentially alter signaling. To address this concern we ran the control experiment of perfusing with NaF alone and showed that there was no significant increase in PKA phosphorylation of RyR2. This indicates that, even if NaF activates adenylyl cyclase, as has been reported (38), there is no significant increase in PKA phosphorylation of RyR2 unless isoproterenol is added. Nevertheless, we cannot exclude the possibility that in some experiments part of the increase in PKA phosphorylation of RyR2 and PLB observed when NaF is added to the perfusate can be due to activation of adenylyl cyclase in addition to inhibition of phosphatases. However, as long as the appropriate controls are performed with each experiment, it should be possible to identify the contribution of NaF induced activation of PKA phosphorylation.
All models of heart failure that we have examined exhibit PKA
hyperphosphorylation of RyR2 and dissociation of FKBP12.6 (16, 28, 36,
37). In end-stage human ischemic and idiopathic dilated
cardiomyopathies, canine pacing-induced heart failure, and in the rat
PMI model (shown here), there are also reductions of PP1 and PP2A in
the RyR2 macromolecular complex. One implication of these findings is
that the reduction of the phosphatases in the RyR2 macromolecular
complex will tend to decrease the rate of dephosphorylation and
contribute to the PKA hyperphosphorylation of RyR2 that is observed,
even when the -ARs may be down-regulated or desensitized.
The hypophosphorylation of PLB in PMI heart failure indicates that Ca2+ pumping into the SR will be decreased both by PLB-mediated inhibition of SERCA2a activity and by reduced levels of SERCA2a. The combination of PKA hyperphosphorylation of RyR2, which results in "leaky" SR Ca2+ release channels, decreased SERCA2a and PLB hypophosphorylation in heart failure can combine to contribute to decreased SR Ca2+ content (39) and decreased [Ca2+]i transients (40).
When the RyR2 macromolecular complexes are depleted of FKBP12.6, the channels exhibit partial openings known as subconductance states. However, when RyR1 and FKBP12 were co-expressed in insect cells that lack endogenous FKBP12, these subconductance states were eliminated (6), and when FKBP12.6 was removed from RyR2 with FK506 or rapamycin subconductances appeared (8). Our results, indicating a cellular function for FKBP12/12.6 in the regulation of RyR1/RyR2 and establishing that functional Ca2+-release channels are macromolecular complexes comprised of RyR and FKBP, have been confirmed by other laboratories (41-43). In addition to stabilizing RyR channels, FKBPs are required for coupled gating (26, 27). Coupled gating between RyR channels allows physically connected RyR channels to gate simultaneously (26, 27). Mathematical modeling of cardiac Ca2+ signaling (44) suggests that the presence of coupled gating is particularly important in terminating SR Ca2+ release from the large RyR2 clusters found in heart cells.
PKA phosphorylation of RyR2 increases the open probability of individual RyR2s in a cluster or Ca2+ release unit (CRU) such that for a given trigger Ca2+ the CRU is more likely to be activated and release SR Ca2+. RyR2 channels are homotetramers comprised of four RyR2 subunits each of which binds one molecule of FKBP12.6 and contains a single PKA phosphorylation site (serine 2809) (16). When serine 2809 on RyR2 is PKA-phosphorylated, FKBP12.6 is dissociated from RyR2. The four equivalent PKA phosphorylation sites on each RyR2 channel provide the basis for a graded physiological response (21). In normal hearts, as each of the PKA sites is transiently phosphorylated there is a progressive physiological increase in RyR2 channel activity and increase EC coupling gain resulting in increased contractility. However, in failing hearts, where on average three or four of the PKA sites are chronically phosphorylated, and the channels are chronically depleted of FKBP12.6, they become destabilized or "leaky" (16). Under these conditions uncoupling of RyR2s would occur due to PKA hyperphosphorylation of RyR2 and depletion of FKBP12.6 from the macromolecular complex. As long as RyR2 is PKA-phosphorylated, FKBP12.6 cannot bind to the channel (1). This reduction in coupled gating (27) would decrease the probability of a CRU being activated by a trigger Ca2+ entering via the L-type channel and would increase the probability of diastolic openings of the SR Ca2+ release channels that could contribute (along with decreased SERCA2a activity) to depletion of SR Ca2+ content and provide signals that activate large inward depolarizing currents (EADs and DADs) that can trigger fatal cardiac arrhythmias associated with sudden cardiac death.
Our data are consistent with reduced coupled gating between individual RyR2 channels in failing hearts for the following reasons: 1) Coupled gating requires FKBP12.6 bound to RyR2, and we have shown that in failing hearts there is a reduction in the amount of FKBP12.6 bound to RyR2 due to PKA hyperphosphorylation. 2) The prolonged decay of calcium sparks in failing hearts can be explained by reduced coupled gating (see Ref. 44). Under normal conditions, the RyR2s in a cluster (CRU) will tend to be close together because of two factors: SR Ca2+ depletion and coupled gating. As coupled gating decreases, other factors being equal, RyR2s will be less well synchronized, and this will produce longer Ca2+ sparks and more variation in the Ca2+ spark duration. 3) Reduced coupling will tend to produce more RyR2 activation at low [Ca2+]i and thus lead to some reduction of the amount of Ca2+ in the SR. This constitutes an increase in the "leak" of Ca2+ from the SR and a reduction in the average amount of Ca2+ in the SR. We suspect that it is the reduced SR Ca2+ content that leads to the reduction in peak Ca2+ spark fluorescence. In addition, the destabilization of individual channels resulting in decreased open and closed dwell times and subconductance states, due to the PKA phosphorylation-induced reduction in FKBP12.6 bound to the channels, may contribute to the alterations in spark characteristics as well.
A finding of considerable importance is the discovery that PKA phosphorylation of RyR2 correlates with the degree of cardiac dysfunction in a murine model. In the MMP-1 TG mouse model, which exhibits progressive cardiac dysfunction over time, RyR2 PKA phosphorylation gradually increases with the degree of cardiac dysfunction (Fig. 4A). This finding indicates that PKA phosphorylation of RyR2 may be an early finding in the progression to heart failure.
We found that channels from the MMP-1 and 2-AR transgenic mice that
were PKA-hyperphosphorylated exhibited the same alterations in
single-channel function (Fig. 5) as RyR2 channels isolated from failing
canine and human hearts (16). These findings are consistent with the
fact that both murine models exhibit cardiac dysfunction and support
the concept that there is a causal relationship between chronic RyR2
PKA hyperphosphorylation and cardiac dysfunction in human HF and in
animal models of HF. The major difference between the channels from
control WT mouse heart and those from either the MMP-1 or
2AR
transgenic mouse hearts is a decrease in open and closed dwell times.
The gating of the channels from both the MMP-1 or
2-AR transgenic
mouse hearts is dramatically different from that seen in the WT mouse
heart controls due to this increase in gating frequency. Indeed, this
is a characteristic finding for channels from failing hearts that are
PKA-hyperphosphorylated and depleted of the stabilizing protein
FKBP12.6 (16).
Our findings provide a new understanding of how stress-activated stimulation of the sympathetic nervous system regulates cardiac Ca2+ handling. Additionally, this work provides insight into the altered Ca2+ signaling that occurs in failing hearts. Although the present work addresses some of the mechanisms by which PKA-mediated signaling regulates RyR2 and Ca2+ homeostasis in heart, there are many additional changes produced by PKA activation besides the ones we have studied.
Our data are consistent with the reports of others regarding the effect of modulation of RyR2 function on EC coupling. A recent study from Bers and colleagues (45) reported that PKA phosphorylation of RyR2 has no effect on Ca2+ spark rate in murine cardiomyocytes at rest. However, these experiments were performed at extremely low cytosolic [Ca2+] levels (for reasons discussed below) of 10 and 50 nM. Thus, the most likely explanation for the failure to observe an effect of PKA phosphorylation on RyR2 is that PKA phosphorylation does not activate RyR2 when cytosolic [Ca2+] is maintained at artificially low levels (RyR2 is a Ca2+-activated channel). This observation should not be extended to the more generalized statement that PKA phosphorylation does not activate RyR2 under physiological conditions that were not examined by Bers and colleagues. They used PLB null cardiomyocytes as well as cardiomyocytes expressing double mutant PLB that cannot be PKA-phosphorylated. They showed that cAMP-induced activation of PKA only modulated Ca2+ sparks when wild type (WT) PLB was present and, therefore, concluded that PKA phosphorylation of RyR2 has no functional effect. There are a number of possible explanations for the failure to observe an effect of PKA phosphorylation of RyR2 in this study, chief among them being the conditions of extremely low cytosolic [Ca2+] as noted above. Additional factors to consider in interpreting the results of Li et al. (45) include the fact that they used streptolysin-O to permeabilize cardiomyocytes. Streptolysin-O treatment resulted in cardiomyocytes with such high resting Ca2+ spark frequency that the studies had to be performed at 10 and 50 nM cytosolic [Ca2+], which is so low that normal RyR2 would be tightly shut. The presence of a high Ca2+ spark rate under their control conditions suggests that the SR was leaky and possibly damaged. Moreover, both the PLB null cells and the PLB double mutant are extreme cases in which there is either no PLB-mediated inhibition of SERCA2a or maximal PLB-mediated inhibition of SERCA2a. It is possible that under such extreme conditions the effects of PKA phosphorylation on RyR2-mediated SR Ca2+ release could be masked (e.g. by a drastic increase in SERCA2a activity that might reduce Ca2+ sparks or drastic decrease in SERCA2a activity that could impair SR Ca2+ uptake despite the fact that the authors attempted to control for these effects). An additional complexity arises due to the time dependence and [Ca2+]i dependence of the effects of PKA phosphorylation of RyR2. Valdivia et al. (19) showed that, at low [Ca2+]i, the actions of PKA phosphorylation on RyR2 only were minimal. However, when [Ca2+]i is rapidly increased (e.g. to 10 µM, see Ref. 44), there is a remarkable increase in RyR2 Po, and this increase in Po is much greater in PKA-phosphorylated RyR2. Furthermore, with maintained elevated [Ca2+]i (which does not happen under physiological conditions) the effects of PKA phosphorylation are to reduce the Po of RyR2. Thus, under the physiological conditions with only brief (~0.5-10 ms) elevations of "subspace" [Ca2+], the effects of PKA phosphorylation are to increase the Po of RyR2.
The finding that acute administration of isoproterenol decreases Ca2+ spark heterogeneity (46) is also consistent with our data. The increased Po of the RyR2 produced by PKA phosphorylation enhances the likelihood that a RyR2 cluster will be triggered by a local Ca2+ influx via dihydropyridine receptor (see above and Ref. 19). Additional rapid actions of PKA phosphorylation that also will produce this same "synchronization" action of isoproterenol are increased uptake of Ca2+ by the SR and increased SR Ca2+ content. Increase in luminal Ca2+ also increases RyR2 Po (47, 48). Additional studies will be needed to determine how these multiple actions of PKA will effect synchronization, [Ca2+]i transients, and Ca2+ sparks. Recent data from an FKBP12.6 null mouse (49) showing a significant increase in decay time of Ca2+ sparks in males are also consistent with our present findings.
Our earlier work showing that PKA activation may improve EC coupling in SS Dahl rat heart cells that exhibit signs of cellular heart failure (30) is also consistent with our present data. Indeed, the apparent paradox is resolved by our new findings. As noted in the results, the absence of NaF in the isolation medium in the previous study may have led to reversal of RyR2 PKA phosphorylation and of the FKBP12.6 dissociation. This would have the effect of restoring the isolated cells to the non-heart failure phenotype with respect to RyR2 function and explains why addition of PKA activators would have a beneficial effect.
The present study provides the first evidence of isoproterenol-induced
RyR2 PKA phosphorylation in vivo, the importance of phosphatase inhibition in studies designed to measure
RyR2-dependent Ca2+ signaling in cardiomyocytes
isolated from failing hearts, and the correlation between progressive
PKA phosphorylation of RyR2 and cardiac dysfunction. Moreover, we have
presented our findings in the context of other recent studies. In so
doing we propose a unifying concept of the role that defects in
Ca2+ signaling, due to alterations in both Ca2+
release and reuptake, can play in the pathogenesis of heart failure and
cardiac arrhythmias.
![]() |
ACKNOWLEDGEMENTS |
---|
We thank P. Bideaux for performing the
surgery in the rat infarct model and W. Koch and R. Lefkowitz for
generously providing the 2-adrenergic receptor transgenic mice.
![]() |
FOOTNOTES |
---|
* This work was supported in part by grants (to A. R. M., W. J. L., and J. D.) from the National Institutes of Health and the Whitaker Foundation (to S. R.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
§§ The Doris Duke Charitable Foundation Distinguished Clinical Scientist. To whom correspondence should be addressed: Center for Molecular Cardiology, Box 65, Columbia University College of Physicians & Surgeons, Rm. 9-401, 630 W. 168th St., New York, NY 10032. Tel.: 212-305-0270; Fax: 212-305-3690; E-mail: arm42@columbia.edu.
Published, JBC Papers in Press, October 24, 2002, DOI 10.1074/jbc.M207028200
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ABBREVIATIONS |
---|
The abbreviations used are: EC, excitation-contraction; PKA, protein kinase A; RyR2, ryanodine receptor/calcium release channel; PLB, phospholamban; PP1 and PP2A, phosphatase 1 and 2A; FKBP, cytosolic binding protein for the immunosuppressant drugs FK506 and rapamycin; AR, adrenergic receptor; SR, sarcoplasmic reticulum; SERCA2a, SR Ca2+ reuptake pump; EAD, early after depolarization; DAD, delayed after depolarization; PKI, PKA inhibitor; PMI, post-myocardial infarction; ANOVA, analysis of variance; MMP-1, matrix metalloproteinase type 1; WT, wild type; TG, transgenic; CRU, Ca2+ release unit; HF, heart failure; Iso, isoproterenol; PIPES, piperazine-N,N'-bis(2-ethanesulfonic acid).
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