From the Department of Chemistry, Leiden University,
¶ Leadd BV, and ** Department of Molecular Cell
Biology, Leiden University Medical Center, 2300 RA Leiden, The
Netherlands
Received for publication, October 22, 2002, and in revised form, December 18, 2002
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ABSTRACT |
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The chicken anemia virus-derived Apoptin
protein induces tumor-specific apoptosis. Here, we show that
recombinant Apoptin protein spontaneously forms non-covalent globular
aggregates comprising 30 to 40 subunits in vitro. This
multimerization is robust and virtually irreversible, and the globular
aggregates are also stable in cell extracts, suggesting that they
remain intact within the cell. Furthermore, studies of Apoptin
expressed in living cells confirm that Apoptin indeed exists in large
complexes in vivo. We map the structural motifs responsible
for multimerization in vitro and aggregation in
vivo to the N-terminal half of the protein. Moreover, we show
that covalently fixing the Apoptin monomers within the recombinant
protein multimer by internal cross-linking does not affect the
biological activity of Apoptin, as these fixed aggregates exhibit
similar tumor-specific localization and apoptosis-inducing properties
as non-cross-linked Apoptin. Taken together, our results imply that
recombinant Apoptin protein is a multimer when inducing apoptosis, and
we propose that this multimeric state is an essential feature of its
ability to do so. Finally, we determine that Apoptin adopts little, if
any, regular secondary structure within the aggregates. This surprising
result would classify Apoptin as the first protein for which, rather
than the formation of a well defined tertiary and quaternary structure,
semi-random aggregation is sufficient for activity.
Apoptin is a protein from chicken anemia virus
(CAV)1 that induces apoptosis
in transformed chicken cells (1). It has 121 amino acids and no known
functional or sequence homologues. When the gene encoding Apoptin is
transduced into cultured cells, the expressed Apoptin protein also
induces apoptosis in a wide range of transformed human cell lines (2).
However, non-transformed, normal human primary cell types are not
killed. Transformed and non-transformed cells differ markedly in the
subcellular localization of expressed Apoptin. In transformed cells,
Apoptin migrates to the nucleus, whereas in non-transformed cells it is
retained mainly within the cytoplasm. Although nuclear localization of
Apoptin appears to be essential for apoptosis induction in transformed cells (3), the presence of Apoptin in the nucleus alone is not
sufficient to induce apoptosis in normal
cells.2 Two clusters of basic
residues (Lys82-Arg89,
Arg111-Arg120) near the C terminus of
Apoptin have been implicated as a nuclear localization signal.
To correlate Apoptin's biological activity with its structure and
biophysical properties, we studied several bacterially expressed, tagged recombinant Apoptin proteins. First, because maltose-binding protein (MBP) fusion constructs can be effective in solubilizing aggregation-prone recombinant proteins, we constructed N-terminally MBP-tagged Apoptin (4, 5). Second, because histidine tagging can allow
efficient refolding of protein immobilized on
Ni2+-chelating resin (6), we also constructed C-terminally
hexahistidine-tagged Apoptin.
MBP-Apoptin fusion constructs were shown to be biologically active and
retain their distinct behavior in normal and tumor cells upon
microinjection.3 Here, we
addressed Apoptin's biophysical and structural properties and how
these relate to its biological function and found it to be biologically
active as a higher order multimer. However, no evidence was found for
an established regular structure.
Cloning of Apoptin Expression Vectors (See Table I for the
Expressed Constructs)
pET-22bVp3, C-terminal Hexahistidine Fusion of Full-Length
Apoptin (Apoptin-H6)--
The Apoptin open reading
frame (ORF) was amplified by PCR from pET-11aVp3, which contains the
Apoptin coding sequence (nucleotides 427-868 in the CAV genome). The
purified PCR fragment was cloned at NdeI and NotI
in pET-22b (Novagen), in which a T7lac promoter, inducible
with isopropyl- pET-22bVp3(1-69)H6, N-terminal 69 Residues of
Apoptin with C-terminal Hexahistidine Tag
(Apoptin(1-69)-H6)--
The N-terminal domain of Apoptin
(ORF nucleotides 1-207) was amplified by PCR and cloned in pET-22b at
NdeI and AvaI.
pMalTBVp3, N-terminal Fusion of MBP and Full-length Apoptin
(MBP-Apoptin)--
The Apoptin ORF was cloned at 5' BamHI
and 3' SalI in pMalTB, downstream of the
Escherichia coli MalE ORF, which codes for MBP. pMalTB is derived from pMal-c2 (New England Biolabs), and the peptide linker between MBP and its fusion partner contains a
thrombin consensus site (-LVPR pMalTBVp3dC69H6 MBP Fusion of N-terminal 69 Residues
of Apoptin with C-terminal Hexahistidine Tag
(MBP-Apoptin(1-69)-H6)--
The truncated Apoptin(1-69)
ORF was amplified from pET-22bVp3(1-69)H6, including the
downstream T7 terminator of pET-22b, and cloned into pMalTB at
BamHI and SalI.
pMalTBVp3dN66, MBP Fusion of C-terminal 56 Residues of Apoptin
(MBP-Apoptin(66-121))--
The truncated Apoptin(66-121) ORF,
including the stop codon, was cloned in pMalTB at BamHI
and SalI.
pcDNA3.1mychis( pcDNA3.1mychis(
All clones were confirmed by automated fluorescent sequencing.
Table I summarizes the protein
constructs we generated.
INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
MATERIALS AND METHODS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
-D-1-thiogalactoside (IPTG), controls expression.
GS-). Expression is controlled by an
IPTG-inducible Ptac promoter.
)-MVp3--
The full MBP-Apoptin ORF,
including the stop codon, was amplified by PCR from pMalTBVp3 and
cloned at NheI and KpnI in
pcDNA3.1mychis(
) type B (Invitrogen). Expression is
controlled by a constitutive cytomegalovirus promoter.
)-MVp3(80-121)--
pcDNA3.1mychis(
)-MVp3
was digested with BamHI and KpnI, removing the
full Apoptin ORF. Subsequently, the truncated Apoptin(80-121) ORF was
amplified by PCR and cloned into the linearized vector at
BamHI and KpnI.
Apoptin expression constructs
Bacterial Expression of Apoptin Constructs
Medium to large scale expression was performed in either of two
set-ups as follows: 1) 500 ml of expression medium in a 2-liter three-baffled flask (Bellco Glass Inc.) (250 rpm, 37 °C); or 2) a
1.8-liter bench-top fermentor (Biospec Products Inc.) (maximum agitation and aeration, 37 °C). In both cases, a few drops of anti-foam A (Sigma) were added directly prior to inoculation. For
expression of MBP fusion proteins, the LB medium was supplemented with
0.2% glucose to suppress the expression of endogenous E. coli -amylases, which might interfere with affinity
purification of the fusion protein. E. coli
BL21(
DE3) CaCl2-competent cells were transformed with
one of the Apoptin constructs described above. A single transformant
colony was grown overnight in LB (+0.2% glucose), supplemented with
200 µg/ml carbenicillin (Duchefa) and if necessary with 0.1 mM ZnSO4. After resuspending the cells in fresh
medium, a volume of expression culture (LB + 0.2% glucose, +100
µg/ml carbenicillin, ±0.1 mM ZnSO4) was
inoculated 1 in 50. Upon reaching an A600 of
~0.8 (2 to 3 h), expression was induced by adding 1 mM IPTG. After 3 to 4 h (final
A600 = 3 to 5), the cells were harvested and
lysed using a BeadBeater (Biospec Products Inc.). Lysis buffers were as
follows: 1) for MBP, MBP-Apoptin, MBP-Apoptin(1-69)-H6,
and MBP-Apoptin(66-121): 25 mM HEPES, pH 7.4, 500 mM NaCl, 10% glycerol, 5 mM reduced
glutathione (GSH) (Sigma), 2 mM MgCl2, 1×
protease inhibitor mixture (EDTA-free) (Roche Molecular
Biochemicals), DNase I (10 to 50 µg/ml) (Roche Molecular
Biochemicals); 2) for Apoptin-H6: 1× PBS, 2 mM
MgCl2, 1 mM phenylmethylsulfonyl fluoride
(Invitrogen), DNase I; 3) Apoptin(1-69)H6, 50 mM KPO4, pH 7.4, 2.5 mM imidazole
(Fluka), 300 mM NaCl, 2 mM MgCl2,
DNase I, protease inhibitor mixture, 2.5 mM GSH. The glass bead/lysate slurry was filtered (Whatmann 3MM) and centrifuged at
29,000 × g for 15 min. Finally, lysates were fully
cleared by filtering over a 0.22-µm filter.
Purification of E. coli-expressed Protein
MBP-Apoptin and MBP-Apoptin(66-121)-- The cleared lysate of a 1-liter expression culture was passed twice over an amylose column (2.5 × 20 cm), equilibrated in 20 mM HEPES, pH 7.4, 500 mM NaCl, 10% glycerol, 1 mM EDTA, or 0.1 mM ZnSO4, under gravity flow at 4 °C. The column was washed with 5 column volumes (CV) of 20 mM HEPES, pH 7.4, 1 M NaCl, 1 mM EDTA, or 0.1 mM ZnSO4 followed by 5 CVs of 20 mM HEPES, pH 7.4, 50 mM NaCl, 1 mM EDTA, or 0.1 mM ZnSO4. The column was then eluted with 20 mM HEPES, pH 7.4, 50 mM NaCl, 1 mM EDTA, or 0.1 mM ZnSO4, 10 mM maltose (Fluka). For all MBP fusion proteins, phenylmethylsulfonyl fluoride (0.5 mM) was directly added to the eluate to prevent degradation by traces of E. coli proteases. After filtering, the eluate was loaded on an analytical cation exchange column (UNO-S12) (Bio-Rad), equilibrated at 3 ml/min in 20 mM HEPES, pH 7.4, 50 mM NaCl, 1 mM EDTA, or 0.1 mM ZnSO4. After extensive washing, the column was eluted with a 200-ml linear gradient (50-1000 mM NaCl, 5-ml fractions). Appropriate fractions were pooled and dialyzed against PBS (CelluSep T1 membranes, molecular mass cut-off 3.5 kDa; Membrane Filtration Products Inc.). MBP-Apoptin and MBP-Apoptin(66-121) were concentrated on a Biomax 5K or 10K Ultrafree spin filter (Millipore) to 40 and 10 mg/ml, respectively. As a rule, solutions of MBP-Apoptin fusion proteins were never subjected to freeze/thawing and stored at 4 °C for up to one month. We noticed that MBP-Apoptin, but not MBP alone, formed insoluble aggregates when incubated at ~1 mg/ml in PBS, 1 mM EDTA, 1% (32 mM) N-octylthioglucoside (OG) (Roche Molecular Biochemicals) for 1 h at 30 °C (data not shown). Only a small amount of MBP-Apoptin remained in solution under these conditions, as tested by Bradford protein assay (Bio-Rad). We did not observe such an effect when OG was replaced by CHAPSO (0.5%; Sigma) or Triton X-100 (1%; Roche Molecular Biochemicals).
Apoptin-H6--
Starting with a 1.8-liter
expression, the pellet after lysis was resuspended in PBS, 0.5% Triton
X-100 (Roche Molecular Biochemicals) and stirred at room temperature
for 1 h. The suspension was then centrifuged at 29,000 × g for 20 min, after which the inclusion bodies were washed
once with PBS. They were then resuspended in 50 mM HEPES,
pH 7.4, 20 mM glycine, 2 mM EDTA, 20 mM DL-dithiothreitol, 8 M urea
(molecular biology grade) (Invitrogen) and stirred overnight at
4 °C. The cleared supernatant was then loaded on a UNO-S12 column,
equilibrated at 3 ml/min in 20 mM KPO4, pH 7.4, 2.5 mM imidazole, 2 mM GSH, 6 M
urea. After washing, the column was eluted with a 160-ml linear
gradient (0-1 M NaCl, 5-ml fractions). Appropriate fractions were pooled and stored at 80 °C if not used immediately afterward. UNO-S12-cleaned Apoptin-H6 was mixed with a
suspension of 10 to 15 ml of Ni2+-nitrilotriacetic acid
(NTA)-agarose (Qiagen), equilibrated in 20 mM
KPO4, pH 7.4, 2.5 mM imidazole, 500 mM NaCl, 2 mM GSH, 6 M urea, and
stirred at 4 °C for 2 h. The resin was packed into a column,
which was subsequently washed with 5 CVs of 20 mM
KPO4, pH 7.4, 20 mM imidazole, 500 mM NaCl, 2 mM GSH, 6 M guanidinium hydrochloride (Invitrogen). The denaturant was then removed, in one
step, by washing with 5 CVs of 20 mM KPO4, pH
6.5, 5 mM imidazole, 400 mM NaCl, 2 mM GSH, 2 mM MgCl2. The protein was
eluted with the same buffer, supplemented with 500 mM
imidazole. All Ni2+ traces were removed completely by
adding 5 mM EDTA to the eluted protein and dialyzing it
against 20 mM KPO4, pH 6.5, 400 mM
NaCl, 2 mM MgCl2. Refolded
Apoptin-H6 was concentrated on a Centricon YM3 filter
(Amicon) to 10 mg/ml. Solutions of refolded Apoptin-H6 were
never subjected to freeze/thawing and were stored for up to one month
at 4 °C.
MBP-Apoptin(1-69)-H6-- The cleared lysate of a 1-liter expression was applied to an amylose column, as described above. After washing, the fusion protein was eluted in 50 mM KPO4, pH 7.4, 2.5 mM imidazole, 300 mM NaCl, 10 mM maltose. The eluate was filtered and loaded on a Ni2+-NTA-agarose column (1.5 × 5 cm), which was washed with 50 mM KPO4, pH 7.4, 30 mM imidazole, 300 mM NaCl. The protein was eluted with 50 mM KPO4, pH 7.4, 300 mM imidazole, 300 mM NaCl and dialyzed against PBS, 0.5 mM EDTA.
MBP--
Non-fused MBP was purified from IPTG-induced
E. coli BL21(DE3) transformed with pMalTB.
After binding to amylose, the column was washed with 20 mM
Tris-HCl, pH 7.4, 1 mM EDTA. MBP was then eluted with 20 mM Tris-HCl, pH 7.4, 1 mM EDTA, 10 mM maltose and loaded on an analytical anion exchange
column (UNO-Q1; Bio-Rad). The column was eluted with a 20-ml linear
gradient (0-1 M NaCl, 1-ml fractions). MBP-containing
fractions (1 to 5 mg/ml) were dialyzed against PBS and then
flash-frozen in liquid nitrogen and stored at
20 °C.
Apoptin(1-69)-H6-- The cleared lysate of a 1-liter culture was loaded on a 5-ml Ni2+-NTA-agarose (1.5 × 3 cm) column. The column was washed with lysis buffer, supplemented with 100 mM imidazole, and then eluted with 20 mM KPO4, pH 7.4, 500 mM imidazole, 500 mM NaCl. After adding EDTA, the eluate was dialyzed to 20 mM KPO4, pH 6.5, 400 mM NaCl, 2 mM MgCl2. Apoptin(1-69)-H6 was concentrated on a Centricon YM3 filter (Amicon) to 10 mg/ml.
Protein Concentration Determination
All protein concentrations were determined from A280. The extinction coefficient of MBP-Apoptin and Apoptin-H6 at 280 nm (mg/ml/cm) was 1.0 ± 0.1 and 0.10 ± 0.01, respectively. The molecular mass of MBP-Apoptin and Apoptin-H6 was taken as 55.8 and 14.5 kDa per monomer, respectively.
Size Exclusion Chromatography
Samples were run as follows: 1) Sephacryl S100 HR (1.0 × 48 cm; Amersham Biosciences), which was calibrated with blue
dextran (>2 MDa; Sigma) and Ponceau S (0.76 kDa; Sigma) at a flow rate of 11.5 cm/h in a sample load of 500 µl; or 2) Superose 6 HR 10/30 (Amersham Biosciences), which was calibrated with a size exclusion chromatography calibration kit (Bio-Rad) (670, 158, 44, 17, and 1.35 kDa) at a flow rate of 0.2 to 0.4 ml/min in a sample load of 100 µl.
For the second set of conditions (Superose 6 HR), linear regression analysis of the elution profile of the calibration run
yielded log[molecular mass (kDa)] = 7.773 (0.365)[ml
eluens].
Dynamic Light Scattering
All dynamic light scattering measurements were recorded on a DynaPro-MS/X (Protein Solutions Inc.) at room temperature. Bovine serum albumin was used as a control (0.5 mg/ml in PBS, 0.5 mM EDTA). MBP-Apoptin was measured at 10 µM (monomer concentration) in PBS, 0.5 mM EDTA, and refolded Apoptin-H6 at 35 µM (monomers) in 20 mM KPO4, pH 6.5, 400 mM NaCl, 2 mM MgCl2. Per experiment, between 20 and 40 measurements (10-s intervals) were collected. The hydrodynamic radius of the particle (RH) was determined by averaging the results of at least three separate experiments. Protein molecular mass was estimated from the RH by the equation, MM (kDa) = [1.68 × RH]2.34.
Circular Dichroism Spectroscopy
Far-UV circular dichroism spectra were recorded on a Jasco J-715 spectrometer. Spectrometer settings were as follows: wavelength range, 190-260 nm; cell width, 1 mm; scan speed, 50 nm/min; response time, 1.0 s; bandwidth, 1.0 nm; pitch, 0.1 nm. Spectra were averaged over eight acquisitions. Throughout the measurements, the spectrometer was flushed with O2-free N2 at 10 liters/min. Cell temperature was maintained at 20 °C by a Jasco PTC-348WI Peltier element. MBP-Apoptin was dialyzed to 10 mM KPO4, pH 6.5, 0.1 mM EDTA, or 0.1 mM ZnSO4, and refolded Apoptin-H6 was dialyzed to 20 mM KPO4, pH 6.5, 1 mM MgSO4, or 0.1 mM ZnSO4. All samples and buffers were filtered and degassed directly prior to measurement.
Fluorescence Measurements
Fluorescence emission and excitation spectra were recorded on a PerkinElmer Life Sciences LS-50B. Fluorimeter settings were as follows: slit width, 6 nm; scan speed, 120 nm/min; excitation wavelength, 280 nm. Final spectra were obtained by averaging three separate spectra. Background emission and excitation spectra were recorded of the respective filtered buffers. The concentration of refolded Apoptin-H6 was 55 µM (monomer concentration) in refolding buffer (20 mM KPO4, pH 6.5, 400 mM NaCl, 2 mM MgCl2). The concentration of free tyrosine (L-Tyr) was 7.5 µM in Apoptin-H6 dialysis buffer, pH 6.5, and 500 µM in 50 mM Na3PO4, pH 12, 1 mM EDTA.
Scanning Force and Electron Microscopic Analysis
Biotin Labeling-- Fresh MBP-Apoptin (20 mg/ml, 0.1 M NaHCO3, pH 8.3) was incubated with 5 mM sulfo-NHS-LC-biotin (Molecular Probes, Inc.) at room temperature for 3 h. The reaction was terminated by adding 10 mM ethanolamine. To remove unincorporated label, the labeled protein was passed over a PD-10 desalting column (Amersham Biosciences), equilibrated in 20 mM HEPES, pH 7.4, 0.1 mM EDTA.
Electron Microscopy-- Both labeled and unlabeled MBP-Apoptin (30 mg/ml, in 20 mM HEPES, pH 7.4, 0.1 mM EDTA) were filtered over 0.22-µm spinfilters (Ultrafree-MC; Millipore) and adsorbed to a carbon-coated polioform layer grid. Both samples were incubated with concentrated streptavidin-gold conjugate (gold particle diameter, 5 nm) (Kirkegaard & Perry Laboratories Inc.) and stained with 3% uranyl acetate. Electron microscopy was performed on a Philips TEM 410 transmission electron microscope.
Scanning Force Microscopy-- 90 ng of purified MBP-Apoptin (in 10 µl of 5 mM HEPES, pH 7.9, 3 mM KCl, 5.5 mM MgCl2) was incubated at 37 °C for 15 min and then deposited on a disc of freshly cleaved mica (Ted Pella Inc.). After 20 s, the mica was gently rinsed with high pressure liquid chromatography water. Excess water was removed, and the disc was dried with a steady flow of 0.22 µm of filtered air. Images were acquired on a Nanoscope IIIa (Digital Instruments Inc.), operating in tapping mode in air with a type E scanner. Silicon tips were obtained from Digital Instruments.
SDS-PAGE and Western Blotting
SDS-PAGE--
Protein samples were run on 7.5-15% SDS-PAGE
gels, either under reducing or non-reducing conditions, i.e.
with or without -mercaptoethanol in the sample buffer. 1× SDS-PAGE
sample buffer contained 20 mM Tris-HCl, pH 6.8, 0.01%
bromphenol blue, 1% SDS, 10% glycerol, 1%
-mercaptoethanol. Gels
were stained with Coomassie Brilliant Blue (Bio-Rad).
Western Blotting and Dot Blotting--
Samples were run on
SDS-PAGE and blotted onto Immunoblot polyvinylidene difluoride
membranes (Bio-Rad) or dot blotted directly onto polyvinylidene
difluoride after denaturation in 1× SDS-PAGE sample buffer (95 °C
for 5 min). Blots were probed with the anti-Apoptin monoclonal antibody
111.3 (epitope, residues 18 to 23) (2) or with the polyclonal
anti-Apoptin antibody VP3-C, which has an epitope range comprising
residues 79 to 90 (9). Blots were subsequently incubated with an
appropriate horseradish peroxidase-conjugated secondary antibody and
developed by enhanced chemiluminescence.
Fluorescent Zinc Assay
Calibration Curve-- A concentration range of ZnSO4 (1 to 20 µM) (Fluka) was prepared in assay buffer (50 mM HEPES, pH 7.4, 0.2% SDS), to which 200 nM FluoZin-1 (Molecular Probes) was added to from a 2 mM stock solution in water. Fluorescence spectra were recorded after 15 min of equilibration at room temperature. Spectra were adjusted for background fluorescence, which was determined from a sample of FluoZin-1, to which 5 mM EDTA had been added. Fluorimeter settings were as follows: slit width, 4 nm; scan speed, 120 nm/min; excitation wavelength, 495 nm.
Sample Preparation-- Protein samples were as follows. 1) MBP-Apoptin was expressed in the presence of 0.1 mM ZnSO4 and purified without using EDTA. 2) MBP-Apoptin, expressed without additional Zn2+ and treated with EDTA, was incubated with a 10-fold molar excess of ZnSO4 overnight at 4 °C (in PBS). Both samples were desalted on a PD-10 column, equilibrated in 50 mM HEPES, pH 7.4, and then denatured in 0.5% SDS (95 °C for 5 min). After cooling to room temperature, the samples were diluted in assay buffer to 10 µM final protein concentration (monomers) and 0.2% final SDS concentration. Fluorescence spectra were recorded after addition of FluoZin-1.
Fluorescent Labeling and Chemical Cross-linking
Fluorescent Labeling--
The number of solvent-exposed Cys
residues per MBP-Apoptin monomer was determined using
5,5'-dithiobis(2-nitrobenzoic acid) (Sigma) (10). Fresh MBP-Apoptin (5 mg/ml, PBS) was incubated with 2 mM of
fluorescein-5-maleimide (Molecular Probes), diluted from a freshly
prepared 20 mM stock solution in 50 mM
Na3PO4, pH 12, and incubated overnight at
4 °C in the dark. The labeling reaction was stopped by adding 10 mM -mercaptoethanol, after which unincorporated label
was removed by passing the protein over a PD-10 column (Amersham
Biosciences), equilibrated in PBS. The level of label incorporation was
determined from the equation, [A/EC] × [MM/C], where A is the absorbance of
the label, C is the concentration of labeled protein (in
mg/ml), EC is the molar extinction coefficient of the label
(cm/M), and MM is the molecular mass of the protein
(in Da).
Cross-linking--
Following labeling with
fluorescein-5-maleimide, the protein (3 mg/ml) was incubated with
0.05% glutaraldehyde, diluted from a 8% stock solution in water
(grade I; Sigma), for 3 min at 30 °C (in the dark). The reaction was
stopped by adding 50 mM -alanine (Fluka). After 20 min,
the protein was passed over a PD-10 column, equilibrated in PBS.
Incubation of Recombinant MBP-Apoptin in Saos-2 and VH10 Lysates in Low Detergent Buffer
Saos-2 cells, which are human tumor cells derived from osteosarcoma, and VH10 cells, which are normal human fibroblasts, were grown to around 50% confluency. The cells were washed with cold PBS and harvested in ice-cold 25 mM HEPES, pH 7.4, 150 mM KCl, 2 mM MgCl2, 5 mM dithiothreitol, 2.5 mM benzamidine hydrochloride (Sigma), 0.25% CHAPSO (Sigma). The suspensions were sonicated on ice, after which insoluble material was removed by centrifugation at 29,000 × g for 20 min. After determining the respective protein concentrations, MBP-Apoptin was added to 5% (w/w), which was estimated to be the average ratio of MBP-Apoptin to cytoplasmic protein in microinjected cells (7, 8). Samples were incubated for 30 min at 30 °C, in the presence of 1 mM ATP and 20 mM MgCl2, and for 2 and 24 h at 4 °C, without additives. Following incubation, samples were fractionated on Superose 6 HR 10/30. Prior to fractionation, any precipitated material was pelleted by centrifugation at 29,000 × g for 20 min, after which the pellets were washed with lysis buffer. All pellets and fractions were dot blotted, using 10 µl per sample, as described above. Dot blots were probed with mAb 111.3.
Microinjection
Saos-2 and VH10 cells were cultured in Dulbecco's modified Eagle's medium containing 10% fetal calf serum, penicillin and streptomycin (Invitrogen). Cells were seeded in 35-mm tissue glass-bottomed culture dishes and grown to 30 to 40% confluency prior to microinjection. During microinjection, cells were incubated in RPMI 1640 medium (25 mM HEPES, pH 7.2, 5% fetal calf serum, penicillin, and streptomycin) at 37 °C. Cells were returned to Dulbecco's modified Eagle's medium directly after microinjection. Microinjection was performed with 0.5-µm microneedles (sterile femtotips II; Eppendorf) under an inverted microscope (Axiovert 135 TV; Zeiss), equipped with a programmable microinjector (IM 300; Narishige Co.) and a joystick hydraulic micromanipulator (MMO-202; Narishige Co.). Fluorescein-labeled, cross-linked MBP-Apoptin (3 mg/ml, in PBS) was combined with lysine-fixable rhodamine-dextran (Molecular Probes) to mark injected cells. Directly prior to injection, protein samples were filtered over 0.22 µm or centrifuged at 15,000 × g for 15 min. An injection pressure of 0.5 to 1.0 pounds/square inch, and an injection time of 0.2 to 0.5 s was used. For analysis of apoptotic activity, 100 cells were injected per dish.
Transient Transfection and Protein Extraction
Saos-2 cells were seeded in 9-cm plates at 20% confluency and
transfected with 7 µg of DNA using FuGENE 6.0 (Roche Molecular Biochemicals) according to the manufacturer's protocol,
pcDNA3.1mychis()-MVp3, pcDNA3.1mychis(
)-MVp3 (80-121) (3:1
µl/µg FuGENE:DNA ratio), or mock-transfected. For analysis of
protein solubility, cells were trypsinized and washed with 1× PBS 2 days after transfection. Cells were pooled from two dishes per
construct and resuspended in 200 µl of 20 mM Tris-HCl, pH
7.5, 250 mM NaCl, 5 mM EDTA, 0.1% Triton
X-100, 20 mM
-glycerophosphate, 5 mM NaF, 5 mM GSH, 2× protease inhibitor mixture (Roche Molecular
Biochemicals), and left on ice for 30 min. Cell debris and the bulk of
the insoluble protein fraction were pelleted by centrifugation at
10,000 × g for 20 min at 4 °C. After separating
supernatant (S10) and pellet (P10), we centrifuged the supernatant at
30,000 × g for 20 min, yielding S30 and P30. Protein
samples were Western blotted, as described above, and blots were
stained with
VP3-C.
(Immuno)fluorescence Microscopy
Microinjection-- 2 and 24 h after injection, cells were fixed sequentially with PBS, 1% freshly prepared formaldehyde (10 min) and then 100% cold MeOH (5 min) and 80% cold acetone (2 min). Fluorescein-labeled, cross-linked MBP-Apoptin was detected by direct fluorescence. The apoptotic condition of individual cells was deduced from their nuclear morphology after staining with 2,4-diamidino-2-phenylindole (DAPI).
Transient Transfection--
In a parallel experiment, Saos-2
cells were grown on coverslips in 9-cm plates and transfected as
described above, fixed using fresh 1:1 methanol:acetone for 5 min, and
stained with a mouse monoclonal antibody that recognized MBP (clone
R29.6; Abcam). An appropriate fluorescein isothiocyanate-labeled
secondary antibody was added, and the cells were mounted in
DAPI/DABCO(1,4-diazabicyclo[2.2.2]octane)/glycerol. Again, apoptotic cells were scored on the basis of their nuclear morphology. Detection of MBP-Apoptin with VP3-C produced comparable results.
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RESULTS |
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Recombinant Apoptin Forms Non-covalent Multimeric Complexes
MBP-Apoptin--
In E. coli, soluble
MBP-Apoptin was expressed with a yield of up to 100 mg per liter of
culture. After affinity chromatography on amylose resin and cation
exchange chromatography, MBP-Apoptin migrated as a stable multimeric
complex, with a molecular mass of 2.5 ± 0.3 MDa, on an analytical
size exclusion chromatography column (Superose 6 HR 10/30) (Fig.
1, A and B). The
molecular mass of the MBP-Apoptin monomer is 56 kDa; see Table I.
Analysis of purified MBP-Apoptin by scanning force microscopy and
electron microscopy showed a uniform population of globular particles
with a radius of ~40 nm (Fig. 2,
A and B). Dynamic light scattering (DLS1) confirmed that the MBP-Apoptin complex is present as
a single solute species with an average RH of
17.9 ± 2.0 nm, which corresponds to a diameter of 36 ± 4.0 nm and an estimated molecular mass of 2.9 ± 0.6 MDa. The
variation in the average RH of the MBP-Apoptin particle
among four separate protein batches remained within experimental error
(16 to 20 nm). The multimerization of MBP-Apoptin was independent of
the protein concentration (0.5 to 25 mg/ml), ionic strength (up to 0.4 M NaCl), and the presence of detergent (0.5% CHAPSO or 1%
Triton X-100). Despite the high stability of the complex, there were no
covalent bonds between individual MBP-Apoptin monomers, because only
monomers could be observed upon boiling in SDS followed by SDS-PAGE
under non-reducing conditions (Fig.
3A). We noticed that
MBP-Apoptin precipitated nearly completely when incubated in 1% OG (in
PBS), a non-denaturing detergent, for 1 h at 30 °C (data not
shown). Because MBP alone did not precipitate under the same
conditions, it appeared that N-octylthioglucoside
selectively destabilized the Apoptin multimer. This result suggests
that hydrophobic interactions play an essential role in the formation
of Apoptin protein multimers.
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In addition to the full-length MBP-Apoptin fusion protein, which migrated as a 58.5-kDa species on SDS-PAGE, three less-abundant expression products of 56.7, 53.3, and 50.1 kDa consistently co-purified with the MBP-Apoptin multimer. All three could be detected with the anti-Apoptin mAb 111.3 (Fig. 3B). These products could not be removed under native conditions, demonstrating that the degradation products were still incorporated into the MBP-Apoptin complex. The linker peptide that connects the MBP and Apoptin moieties contains a thrombin cleavage site. When the fusion protein was digested with thrombin, cleaved-off MBP was released from the complex, whereas the Apoptin moieties remained part of it, indicating that the multimerization behavior of MBP-Apoptin depended entirely on the Apoptin moiety (data not shown).
Apoptin-H6-- In E. coli, inclusion bodies of Apoptin-H6 were expressed with a yield of up to 40 mg per liter of culture. Manipulating growth or lysis conditions did not increase the solubility of Apoptin-H6. After solubilization in 8 M urea, Apoptin-H6 was purified to ~90% homogeneity by cation exchange chromatography under denaturing conditions (6 M urea). It could be refolded with a net efficiency of ~50% in a single step while bound to Ni2+-NTA-agarose. On Superose 6 HR 10/30, refolded Apoptin-H6 migrated as a single species with a molecular mass of 400 ± 50 kDa, independent of protein concentration (1 to 7 mg/ml) (Fig. 1C). The molecular mass of the Apoptin-H6 monomer is 14.5 kDa; see Table I. The two additional peaks of 30 and 10 kDa in the Superose 6 elution profile were unlikely to contain Apoptin as they were not recognized by the Apoptin-specific antibody mAb 111.3. DLS confirmed the particle size, indicating a single solute species with an RH of 8.5 ± 1.0 nm, corresponding to a molecular mass of 500 ± 100 kDa. The variation in complex size among three separate protein batches remained within experimental error (8 to 10 nm).
Like the MBP-Apoptin complex, the Apoptin-H6 complex was non-covalent, as shown by non-reducing SDS-PAGE (Fig. 3C). A number of additional species migrating with apparent molecular masses of 8, 34, and 48 kDa could be detected by mAb 111.3 (Fig. 3D). The smallest mAb 111.3-reactive species (8 kDa) corresponded to an N-terminal fragment lacking the C-terminal hexahistidine tag. As in the MBP-Apoptin complexes, fragments truncated at the C terminus remained associated and co-purified.
Mapping the Regions Required for Multimerization: N- and C-terminal Domains of Apoptin-- To evaluate the ability of fragments of Apoptin to form multimers, Apoptin's N-terminal 69 residues and C-terminal 56 residues (66-121) were cloned separately as MBP fusion proteins. The MBP-Apoptin(1-69) fusion also contained a C-terminal hexahistidine tag to facilitate further purification. In E. coli, soluble MBP-Apoptin(1-69)-H6 was expressed with yields of up to 100 mg per liter of culture. It displayed the same elution profile on size exclusion chromatography as did full-length MBP-Apoptin(1-121), indicating that the complex is about 2.5 MDa in size (Fig. 1D). As with the full-length MBP-Apoptin complex, Apoptin(1-69)-H6 with its MBP moiety cleaved off co-eluted with the intact fusion protein (data not shown).
In E. coli, soluble Apoptin(1-69)-H6 was expressed with a yield of up to 2 mg per liter of culture. After Ni2+-NTA-agarose purification, the homogeneity of Apoptin(1-69)-H6 was ~80%. When the Ni2+-NTA-agarose eluate was fractionated on a Sephacryl S100 HR gel filtration column, the Apoptin(1-69)-H6 could only be recovered from the void volume. This finding indicated that Apoptin(1-69)-H6 formed a complex of at least 100 kDa in size under non-denaturing conditions (data not shown). The molecular mass of the Apoptin(1-69)-H6 is 8.3 kDa; see Table I.
MBP-Apoptin(66-121) was expressed in soluble form to ~50 mg per liter of culture. Purified MBP-Apoptin(66-121) migrated at ~50 kDa on SDS-PAGE. In contrast to the full-length fusion protein, MBP-Apoptin(66-121) consisted exclusively of two species of ~200 and 60 kDa, which may correspond to an equilibrium between a monomer and a di- or trimer (Fig. 1E). The molecular mass of MBP-Apoptin(66-121) is 49 kDa; see Table I. DLS analysis indicated that there was only one solute species present, with an RH of 4.6 ± 0.8 nm, which corresponds to an average molecular mass of 120 ± 50 kDa. Regardless of the precise nature of the two species, this result shows that the C-terminal domain of Apoptin on its own is unable to form the type of higher order multimers such as MBP-Apoptin(1-121) does. Taken together, these results demonstrate that the N-terminal 69 residues of Apoptin contain sufficient structural elements to account for its multimerization behavior.
Apoptin Has Little if Any Ordered Structure
Next, we examined whether recombinant Apoptin protein harbors any secondary or tertiary structure, using circular dichroism (CD) and fluorescence spectroscopy.
CD Spectroscopy--
The far-UV CD spectrum of refolded
Apoptin-H6 (Fig.
4A) showed a very small mean
residue ellipticity ([]MRE) at 222 nm, indicating that
the protein was nearly devoid of
-helical structure (11). Refolded
Apoptin-H6 also contained little
-sheet structure, which could be deduced from a small positive [
]195 and a
small negative [
]216 (11). The k2d program for protein
secondary structure prediction from CD spectra (12) could not assign
any
-helical or
-sheet structure. Although refolded
Apoptin-H6 had little if any secondary structure, it did
not adopt a fully random conformation, which would be characterized by
a large negative [
]190-200 (11). It is possible that
the flexibility of the polypeptide is restricted because of the high
Pro content of Apoptin (12.4%). The negative
[
]200-210 could even suggest the presence of a
poly-L-proline helix, although an accompanying small
positive [
]228 was absent (13). Nevertheless, some of
Apoptin's Pro residues may adopt a helix-like conformation. The broad
negative [
]MRE of Apoptin between 210 and 230 nm was
reminiscent of CD spectra reported for
-turn- or -hairpin-containing
peptides (14, 15). However, the geometry of residues involved in
-turns and -hairpins can vary considerably, making their net
contribution to a CD spectrum poorly quantifiable. In general, the CD
spectrum of refolded Apoptin-H6 was very similar to that of
proteins that are mostly unstructured (16-19).
|
The CD spectrum of MBP-Apoptin confirmed these observations, being not
significantly different from that of MBP on its own (Fig.
4B) (20, 21). First, this result shows that the Apoptin moiety did not perturb the folding of MBP. Second, it demonstrates that
Apoptin did not contribute significantly to the secondary structure
content of the fusion protein. This is consistent with the very low
overall []MRE of refolded Apoptin-H6.
We conclude that Apoptin displays very little regular secondary
structure, but we cannot exclude that some residues adopt a
-strand
or -turn conformation.
Intrinsic Fluorescence of Tyr95--
Even though
Apoptin did not appear to adopt a well defined fold, the Apoptin
polypeptide might still display a certain degree of intra- or
intermolecular order within the multimeric complex. Because
Apoptin-H6 does not contain any Trp residues, the intrinsic fluorescence of its single Tyr95 can act as a structural
probe (22, 23). Tyr95 is outside the multimerization domain
defined by Apoptin's N-terminal residues. The excitation spectrum of
Tyr95 in refolded Apoptin-H6 was very similar
to that of protonated L-Tyr(-OH), with excitation maxima at
280 and 275 nm, respectively (Fig.
5A). However, the fluorescence
emission spectrum of Apoptin's Tyr95 at pH 6.5 closely
resembled that of deprotonated, free tyrosine (L-Tyr(-O)) at pH 12 (Fig. 5B).
This apparent discrepancy can be explained by the effect that
excitation has on the pKa of the Tyr side chain; the pKa of Tyr(-OH) is near 10 in the ground
state but decreases to ~4 upon excitation (24, 25). Because the
emission spectrum of refolded Apoptin-H6 was almost
entirely devoid of Tyr(-OH) fluorescence, an efficient proton transfer
to a nearby proton-accepting group (Glu, Asp, or His) must have
occurred upon excitation of Tyr95. This result indicates
that Tyr95 was hydrogen bonded. The fluorescence yield of
Tyr95 was increased by a factor of 4 to 5 relative to fully
deprotonated and solvent-exposed L-Tyr(-O
)
(at pH 12), which indicates that Tyr95 was at least
partially protected from the solvent. The presence of an internal
hydrogen bond involving the side chain of Tyr95 suggests
that at least one region of the Apoptin polypeptide was able to adopt a
more ordered conformation.
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Apoptin Did Not Bind Zn2+ Despite a Putative Zn2+-binding Motif-- Apoptin contains a potential metal-binding motif comprised of one His (His29) and three Cys residues (Cys30, Cys47, and Cys49). Although this arrangement does not bear a clear resemblance to any known metal-binding motifs, it is possible that binding of a metal ion by Apoptin influences its secondary structure content or multimerization behavior. The most obvious ligand for this motif would be Zn2+. Including Zn2+ in both the expression medium and purification buffers did not affect the expression level, solubility, or net yield of MBP-Apoptin and Apoptin-H6. Furthermore, the CD spectra of MBP-Apoptin and Apoptin-H6 were not significantly altered by the presence of Zn2+ added either after purification or during expression. The presence of Zn2+ affected neither efficiency nor specificity of thrombin digestion of MBP-Apoptin nor the intrinsic fluorescence of refolded Apoptin-H6. Finally, an assay based on the strong increase in fluorescence upon Zn2+ binding of the water-soluble Zn2+-selective chelating dye FluoZin-1 (26) indicated that MBP-Apoptin expressed and purified in the presence of Zn2+ contained less than 0.1 mole of Zn2+ per mole of protein (data not shown). We conclude that recombinant Apoptin does not form a stable complex with Zn2+.
MBP-Apoptin Expressed in Tumor Cells Exists in an Aggregated State
Having established that recombinant Apoptin protein exists exclusively as a remarkably homogenous and highly stable aggregate in vitro, we evaluated the aggregation state of ectopically expressed MBP-Apoptin in human tumor cells (Saos-2). As our data on recombinant MBP-Apoptin(66-121) and MBP-Apoptin(1-69)-H6 suggested that Apoptin's multimerization domain resided in its N-terminal domain, we expressed MBP-Apoptin(80-121) to compare the appearance of aggregated full-length MBP-Apoptin with that of a truncated, putatively non-aggregating MBP-Apoptin construct.
We inspected the morphological appearance of the two expressed
construct in intact cells by immunofluorescence microscopy. Two days
after transfection, a time point preceding significant apoptosis
induction in this cell type, full-length MBP-Apoptin, was seen to form
distinct globular particles in the cytoplasm and nucleus of Saos-2
cells (Fig. 6A). Most
strikingly, a large proportion of the protein accumulated at the
nuclear rim, which may be caused by "congestion" of
MBP-Apoptin particles at or in the nuclear pores. In contrast,
MBP-Apoptin(80-121) had a much more diffuse appearance and was
predominantly located in the nucleus (Fig. 6A). We observed
essentially the same distribution in HeLa cells (data not shown). These
results suggest that full-length MBP-Apoptin becomes aggregated in
tumor cells, whereas MBP-Apoptin(80-121) does not. Moreover, these
results confirm that MBP itself does not aggregate or contribute to the
aggregation of its fusion partner, which is in accordance with the
properties of bacterially expressed MBP-Apoptin fusion proteins.
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In parallel, we determined the aggregation state of both protein constructs in cell extracts by centrifugation and found that the bulk of the extracted full-length MBP-Apoptin could be pelleted at 10,000 × g, indicating that it is or is part of a very dense aggregate (Fig. 6B). Moreover, most of the MBP-Apoptin that remained in the supernatant could be pelleted at 30,000 × g. Under the same conditions, MBP-Apoptin(80-121) was fully soluble (Fig. 6A). Moreover, we verified that recombinant MBP-Apoptin alone did not precipitate upon dilution in lysis buffer (Fig. 6C). We assume that the trace amount of full-length MBP-Apoptin remaining in the supernatant after centrifugation at 30,000 × g corresponds to newly translated polypeptides that were not yet fully aggregated or had been absorbed by detergent micelles. Taken together with the immunofluorescence data, these results imply that aggregation of Apoptin occurs in vivo and that the determinants responsible for aggregation are located in the N-terminal part of the protein.
The Recombinant MBP-Apoptin Protein Complex Is Active as a Multimeric Species
In a separate paper, we show that microinjected recombinant MBP-Apoptin protein induces apoptosis in tumor Saos-2 cells, but not in normal VH10 cells.3 This observation prompted us to examine the role of aggregation of recombinant MBP-Apoptin in apoptosis induction.
We established that recombinant Apoptin aggregates were not dissolved by cellular factors in vitro under physiologically relevant conditions; neither VH10 nor Saos-2 cell lysate affected the size distribution of MBP-Apoptin aggregates in the presence of ATP and Mg2+, as tested by size exclusion chromatography (data not shown). Furthermore, in both Saos-2 and VH10 samples, the characteristic degradation pattern of MBP-Apoptin remained unchanged upon incubation and fractionation. A small amount of untagged Apoptin was present in the starting material, and it remained associated with the intact fusion protein without any changes in ratio (data not shown).
Next, to ensure that MBP-Apoptin aggregates did not dissolve upon
microinjection into living cells, we cross-linked the MBP-Apoptin aggregates by brief incubation with 0.05% glutaraldehyde. The majority
of these cross-links are expected to be between the MBP moieties if the
multimeric fusion protein, as they contain most of the Lys residues.
Cross-linked complexes did not contain detectable amounts of smaller
oligomers or monomers (Fig.
7A). DLS analysis of
cross-linked MBP-Apoptin confirmed the presence of a single particle with an RH of 14.0 ± 1.5 nm. First, this
finding demonstrated that cross-linking caused the MBP-Apoptin complex
to become more compact. Second, it showed that nearly all cross-linking
occurred within complexes, with a negligible number of linkages between different complexes being formed. To follow the fate of MBP-Apoptin complexes upon microinjection, the fluorescent label
fluorescein-5-maleimide was attached to Apoptin's single
solvent-exposed Cys residue prior to glutaraldehyde cross-linking. When
microinjected into the cytoplasm of Saos-2 cells, the
fluorescein-labeled, cross-linked MBP-Apoptin was imported into the
nucleus and induced the same level of apoptosis within 24 h as
non-cross-linked MBP-Apoptin (Fig. 7, B and C). However, the efficiency of nuclear import of cross-linked MBP-Apoptin appeared to be decreased in comparison to non-cross-linked MBP-Apoptin (data not shown),3 which may indicate that some of its
nuclear localization signals are obscured as a result of glutaraldehyde
treatment. In VH10 cells, cross-linked MBP-Apoptin remained in the
cytoplasm and did not induce apoptosis (Fig. 7, B and
D). Clearly, covalent cross-linking did not have any
significant effect on the activity of microinjected MBP-Apoptin,
implying that in vivo dissociation of the recombinant
Apoptin protein multimers is not required for tumor-specific apoptosis
induction.
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DISCUSSION |
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Structure of the Apoptin Complex-- We demonstrated with a range of biophysical techniques that recombinant Apoptin protein forms multimeric globules of a distinct size that contain about 30 monomers each. Our scanning force microscopy and electron microscopy studies indicated that Apoptin multimers have a roughly spherical shape. The presence of an 18-residue linker between MBP and Apoptin in the MBP-Apoptin fusion protein increased its hydrodynamic radius, leading to an overestimation of its molecular mass. When MBP-Apoptin complexes were cross-linked with glutaraldehyde, they became more compact as judged by DLS, presumably because the MBP moieties became firmly attached to each other and to the body of the Apoptin core. On the basis of its hydrodynamic radius, the theoretical molecular mass of the cross-linked MBP-Apoptin complex is 1.6 MDa, corresponding to 30 monomeric subunits. This is fully consistent with the monomer content of refolded Apoptin-H6 we observed.
CD spectroscopy indicated that Apoptin protein multimers contain little if any secondary structure, which may reflect a lack of internal ordering. This result raises the question of how a seemingly disordered Apoptin polypeptide can assemble into a biologically active and homogenous protein multimer. We hypothesize that Apoptin multimers consist of only a limited number of monomer conformations. Our experimental results on recombinant Apoptin protein support such an idea. First, we demonstrated that Apoptin's Tyr95 forms a stable hydrogen bond, which implies that there is at least some internal structure. Second, the CD spectrum of refolded Apoptin-H6 was clearly different from that of a true random coil polypeptide, indicating that its conformational freedom was restricted (11). If Apoptin multimers present an at least partially ordered surface, this characteristic would allow them to interact selectively with cellular factors. Such an interaction could give rise to a particular biological effect, namely the induction of apoptosis in a tumor-specific manner. Furthermore, it could be that small domains of the Apoptin multimer become ordered once recognized by cellular factors. There are precedents for such behavior; for example, high mobility group proteins specifically recognize DNA, yet in the absence of DNA do not adopt a regular conformation (27).
According to secondary structure prediction, Apoptin might fold as an
anti-parallel -sheet between Glu32 and Leu46
with Ala38 and Gly39 oriented in a
-turn or
-hairpin. The CD spectrum of refolded Apoptin-H6 is
consistent with such a short anti-parallel
-sheet. In this motif
(29HCREIRIGIAGITITLSLCGC49), small, mostly
hydrophilic residues alternate with seven Leu and Ile side chains. In
addition, this motif is flanked by a potential metal-binding site
(His29, Cys30, Cys47, and
Cys49). However, we showed that this configuration of Cys
and His residues did not constitute a Zn2+-binding site in
recombinant Apoptin. If this sequence indeed adopts a
-hairpin-type
conformation, the large hydrophobic residues would all protrude from
one of the faces of the hairpin, whereas the hydrophilic residues would
protrude from the other. Such an amphipathic motif could well be
essential for the multimerization properties of Apoptin, perhaps by
allowing the Leu and Ile residues of neighboring Apoptin monomers to
interlock. Our observation that the presence of OG caused MBP-Apoptin
to precipitate does suggest that the multimerization of Apoptin is, at
least to an extent, based on hydrophobic interactions. It may be that
OG is more effective than CHAPSO and Triton in disrupting Apoptin
multimers because of its small size and high micelle concentration
(~9 mM or ~0.3%), allowing it to penetrate the Apoptin
core of MBP-Apoptin.
The Leu/Ile clustering of the potential amphipathic -hairpin is
reminiscent of a Rev- or PKI
like nuclear export signal (NES) (28).
Although the NES sequences of PKI
and of p53 have been reported to
adopt an amphipathic
-helical conformation (29, 30), CD spectroscopy
showed that refolded Apoptin-H6 did not contain any
-helical regions. If indeed the isoleucines and leucines of the
putative NES of Apoptin are part of its multimerization motif, these
residues would, because of their hydrophobic nature, be largely buried
within the multimer. Thus obscured, Apoptin's putative NES would be
likely to have reduced activity.
Activity of the Apoptin Complex-- We established that ectopically expressed, full-length MBP-Apoptin was aggregated in live tumor cells, whereas truncated MBP-Apoptin(80-121) was not. This difference in aggregation state was consistent with our findings on recombinant MBP-Apoptin and truncated versions thereof. Moreover, the distribution of ectopically expressed MBP-Apoptin, including its accumulation at the nuclear envelope, was similar to that of the recombinant protein following microinjection.3 Furthermore, the size of the aggregates formed by full-length MBP-Apoptin in vivo was remarkably regular. Such homogeneity is strongly reminiscent of the narrow size distribution of bacterially expressed MBP-Apoptin. However, ectopically expressed MBP-Apoptin was contained in an aggregate particle that was denser than the recombinant Apoptin protein multimer. Therefore, it is likely that Apoptin expressed in vivo forms a larger aggregate or co-aggregates with other cellular proteins.
Our results suggest that aggregation of Apoptin in vivo does not preclude induction of apoptosis, which would imply that Apoptin does not have to be a properly folded molecule to be active. Moreover, as most if not all ectopically expressed MBP-Apoptin was found to be aggregated, it is very likely that this aggregated state represents the physiological form of Apoptin in tumor cells. Alternatively, it is possible that the protein undergoes a transition between a folded and an unfolded state in vivo upon binding to some cellular factor. We expect that an inducible Apoptin expression system in human tumor cells will allow us to evaluate the molecular mass distribution of ectopically expressed Apoptin in more detail.
Based on the findings reported here, we hypothesize that the multimeric state is the functional form of recombinant MBP-Apoptin, which we propose to be analogous to the aggregated state of ectopically expressed MBP-Apoptin. First, cell lysates could not bring about the efficient dissolution of the Apoptin multimers into monomers or smaller oligomers. Second, chemical cross-linking of MBP-Apoptin did not significantly affect its subcellular localization and apoptosis-inducing ability in human tumor cells. Moreover, cross-linked Apoptin protein multimers did not kill normal cells. Taken together, these results clearly demonstrate that Apoptin does not have to adopt a monomeric form to be active as a tumor-specific apoptosis-inducer. Nevertheless, these results do not formally rule out the possibility that Apoptin might exist as a monomer in vivo. However, it should be noted that both MBP-Apoptin and Apoptin-H6 multimers were found to be remarkably stable. They could not be dissolved under native conditions in vitro, suggesting that no significant amounts of monomeric Apoptin were ever released. Even under denaturing conditions (8 M urea or 6 M guanidinium hydrochloride), the interactions between individual Apoptin proteins were only partially abolished (data not shown). Clearly, the driving force behind multimer formation is dominant both in the intracellular environment of E. coli and while Apoptin-H6 was tethered to Ni2+-chelating resin under refolding conditions, which suggests that multimerization is probably the fate of a significant fraction of in vivo expressed Apoptin, as well. In all, we conclude that aggregated, or multimeric, recombinant Apoptin protein contains the essential features that allow Apoptin, expressed in vivo, to induce apoptosis in a tumor-specific manner. Moreover, we conclude that the lack of secondary structure in recombinant Apoptin protein is likely to reflect, at least to some extent, the intrinsically disordered nature of Apoptin.
There are many examples of proteins that can undergo controlled
denaturation/aggregation or polymerization in vitro
(31-33). In general, controlled aggregation occurs under conditions
that stabilize a particular folding intermediate. The disruption of a
folding pathway in vivo that leads to the accumulation of
such intermediates in intracellular aggregates is linked to several pathological processes. Hence, evolution of protein secondary and
tertiary structure is thought to be aimed, at least in part, on
bypassing such kinetic folding "sinks" of partially folded polypeptides (34). A well documented example of a pathogenic, aggregation-prone protein is huntingtin, the pathogenic effect of which
is characterized by the formation of intranuclear aggregates of
N-terminal huntingtin fragments, an event that is directly linked to
apoptosis induction (35). An example where protein aggregation seems to
represent "gain-of-function" is the human milk protein
-lactalbumin, which forms partially unfolded oligomers that are
imported into the nucleus of tumor and differentiating cells, whereas
the monomeric form is not. Nuclear import of oligomeric
-lactalbumin
was accompanied by induction of apoptosis (36, 37). It has been
suggested that the presence of globular aggregates of misfolded protein
in the cell may lead to cell death if these aggregates are efficient
nucleation sites for fibrous amyloid formation (33). However, the
globular aggregates of recombinant Apoptin did not form recognizable
fibrous amyloid deposits when microinjected into live
cells,3 and such amyloid formation by Apoptin was also not
observed in vitro in the current study. Therefore, the
mechanism of apoptosis induction by Apoptin is clearly different.
Moreover, apoptosis induction by recombinant Apoptin multimers is not a
general cytotoxic effect, as Apoptin does not appear to elicit any
harmful effects when introduced into several different normal primary
human cells.3 Apoptin will exert its pro-apoptotic function
only when a cell has entered the pathway that leads to a transformed
state (38). In that respect, Apoptin clearly differs from proteins that
give rise to aggregation-linked diseases (39).
Apoptin is a viral protein encoded by chicken anemia virus. The virus has a minimal single-stranded DNA genome, and Apoptin's gene fully overlaps with that of the VP2 protein, albeit with a shift in frame (40). One possible function of Apoptin in the replication cycle of CAV is to induce apoptosis of infected chicken thymocytes to release them from the host cell once the viral particles have matured. Transmission of CAV may be enhanced when the virus is encased in or associated with apoptotic bodies, analogous to adenoviral vectors (41). In such a process, the potential co-transmission of Apoptin globules, together with infectious viral particles, may also have a biological function. In this respect it is striking that Apoptin forms a globular particle roughly the size of a virus, so it may have an evolutionary relationship with a viral coat protein. The requirement of a compact genome in CAV has impelled the Apoptin gene to fully overlap with that of VP2 in the viral genome. The obvious price of this compactness is that the sequences of both genes are more strongly constrained. However, if a protein's function requires it merely to aggregate, rather than to adopt a well defined quaternary conformation, the constraints on its sequence will be less stringent.
It is well possible that Apoptin's original function has demanded its
multimerization, which it may have achieved through random aggregation
as outlined above, rather than through the formation of a specific
quaternary structure. If so, Apoptin provides an example of a novel
route for the evolution of protein function. There are several possible
explanations why the multimerization of Apoptin may be essential for
its biological function; e.g. aggregation may stabilize
Apoptin, which in its ill-defined monomeric form may be readily
degradable, or the formation of globular multimers may result in
cooperative binding of Apoptin moieties to certain large ligands or
molecular complexes (DNA, RNA, chromatin, nuclear pores, etc.).
Obviously these possibilities do not exclude one another, underscoring
the likeliness that in the case of Apoptin the classical
structure/function paradigm translates as an aggregation/function paradigm.
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ACKNOWLEDGEMENTS |
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We thank Hans van der Meulen (Leiden University Medical Center) for performing electron microscopic analysis and Remus Thei Dame (Molecular Genetics, Leiden University) for performing scanning force microscopic analysis of MBP-Apoptin. Furthermore, we extend our gratitude to Fred Wassenaar (Leiden University Medical Center) for the donation of the vector pMalTB. We are also grateful to Klaas Kooistra (Leadd B.V.) for culturing VH10 and Saos-2 cells.
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FOOTNOTES |
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* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
§ Contributed equally.
To whom correspondence should be addressed: P. O. Box 9502, 2300 RA Leiden, The Netherlands. Tel.: 31-071-5274213; Fax:
31-071-5274357; E-mail: abrahams@fwncism1.leidenuniv.nl.
Published, JBC Papers in Press, December 19, 2002, DOI 10.1074/jbc.M210803200
2 A. Danen-van Oorschot, Y.-H. Zhang, S. R. Leliveld, J. L. Rohn, M. C. M. J. Seelen, M. W. Bolk, A. van Zon, S. J. Erkeland, J. P. Abrahams, D. Mumberg, and M. H. M. Noteborn, manuscript in preparation.
3 Y.-H. Zhang, S. R. Leliveld, K. Kooistra, C. Molenaar, J. Rohn, H. J. Tanke, J. P. Abrahams, and M. H. M. Noteborn, submitted manuscript.
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ABBREVIATIONS |
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The abbreviations used are:
CAV, chicken anemia
virus;
CV, column volume;
DAPI, 2,4-diamidino-2-phenylindole;
DLS, dynamic light scattering;
IPTG, isopropyl--D-1-thiogalactoside;
mAb, monoclonal
antibody;
MBP, maltose-binding protein;
MM, molecular mass;
NES, nuclear export signal;
NTA, nitrilotriacetic acid;
OG, N-octylthioglucoside;
ORF, open reading frame;
RH, hydrodynamic radius;
PBS, phosphate-buffered saline;
CHAPSO, 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonic
acid.
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REFERENCES |
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---|
1. | Noteborn, M. H., Todd, D., Verschueren, C., de Gauw, H., Curran, W., Veldkamp, S., Douglas, A., McNulty, M., van der Eb, A., and Koch, G. (1994) J. Virol. 68, 346-351[Abstract] |
2. |
Danen-van Oorschot, A. A.,
Fischer, D.,
Grimbergen, J.,
Klein, B.,
Zhuang, S.-M.,
Falkenburg, J.,
Backendorf, C.,
Quax, P.,
van der Eb, A.,
and Noteborn, M.
(1997)
Proc. Natl. Acad. Sci. U. S. A.
94,
5843-5847 |
3. | Zhuang, S.-M., Landegent, J. E., Verschueren, C. A., Falkenburg, J. F., van Ormondt, H., van der Eb, A. J., and Noteborn, M. H. (1995) Leukemia 9 Suppl. 1, 118-120[Medline] [Order article via Infotrieve] |
4. | Kapust, R., and Waugh, D. (1999) Protein Sci. 8, 1668-1674[Abstract] |
5. | Wang, C., Castro, A., Wilkes, D., and Altenberg, G. (1999) Biochem. J. 338, 77-81[CrossRef][Medline] [Order article via Infotrieve] |
6. | Stempfer, G., Holl-Neugebauer, B., and Rudolph, R. (1995) Nature Biotechnol. 14, 329-334 |
7. | Lee, G. (1989) J. Cell Sci. 94, 443-447[Abstract] |
8. | Minascheck, G., Bereiter-Hahn, J., and Bertholdt, G. (1989) Exp. Cell Res. 183, 434-442[Medline] [Order article via Infotrieve] |
9. |
Danen-van Oorschot, A.,
van der Eb, A.,
and Noteborn, M.
(2000)
J. Virol.
74,
7072-7078 |
10. | Riddles, P. W., Blakeley, R. L., and Zerner, B. (1983) Methods Enzymol. 91, 49-60[Medline] [Order article via Infotrieve] |
11. | Rodger, A., and Norden, B. (1997) in Circular Dichroism and Linear Dichroism. Oxford Chemistry Masters (Compton, R. G. , Davies, S. G. , and Evans, J., eds) , pp. 20-22, Oxford University Press, Oxford |
12. | Andrade, M., Chacon, P., Merelo, J., and Moran, F. (1993) Protein Eng. 6, 383-390[Medline] [Order article via Infotrieve] |
13. | Kelly, M., Chellgren, B., Rucker, A., Troutman, J., Fried, M., Miller, A., and Creamer, T. (2001) Biochemistry 40, 14736-14383[CrossRef][Medline] [Order article via Infotrieve] |
14. | Ramirez-Alvarado, M., Blanco, F., Niemann, H., and Serrano, L. (1997) J. Mol. Biol. 273, 898-912[CrossRef][Medline] [Order article via Infotrieve] |
15. | Griffiths-Jones, S., Maynard, A., and Searle, M. (1999) J. Mol. Biol. 292, 1051-1069[CrossRef][Medline] [Order article via Infotrieve] |
16. |
Kriwacki, R.,
Hengst, L.,
Tennant, L.,
Reed, S.,
and Wright, P.
(1996)
Proc. Natl. Acad. Sci. U. S. A.
93,
11504-11509 |
17. | Cregut, D., and Serrano, L. (1999) Protein Sci. 8, 271-282[Abstract] |
18. |
McCutchen-Maloney, S.,
Matsuda, K.,
Shimbara, N.,
Binns, D.,
Tanaka, K.,
Slaughter, C.,
and DeMartino, G.
(2000)
J. Biol. Chem.
275,
18557-18565 |
19. | Adkins, J., and Lumb, K. (2002) Proteins Struct. Funct. Genet. 46, 1-7[CrossRef][Medline] [Order article via Infotrieve] |
20. | Ganesh, C., Shah, A., Swaminathan, C., Surolia, A., and Varadarajan, R. (1997) Biochemistry 36, 5020-5028[CrossRef][Medline] [Order article via Infotrieve] |
21. |
Fischer, M.,
Corringer, P.,
Schott, K.,
Bacher, A.,
and Changeux, J.
(2001)
Proc. Natl. Acad. Sci. U. S. A.
98,
3567-3570 |
22. | Härd, T., Hsu, V., Sayre, M., Geiduschek, E., Appelt, K., and Kearns, D. (1989) Biochemistry 28, 396-406[Medline] [Order article via Infotrieve] |
23. | Ferreira, S., Stella, L., and Gratton, E. (1994) Biophys. J. 66, 1185-1196[Abstract] |
24. | Szabo, A., Lynn, K., Krajcarski, D., and Rayner, D. (1978) FEBS Lett. 94, 249-252[CrossRef][Medline] [Order article via Infotrieve] |
25. | O'Neill, J., and Hofmann, T. (1987) Biochem. J. 243, 611-615[Medline] [Order article via Infotrieve] |
26. | Gee, K., Zhou, Z., Qian, W., and Kennedy, R. (2002) J. Am Chem. Soc. 124, 776-778[CrossRef][Medline] [Order article via Infotrieve] |
27. | Reeves, R. (2001) Gene 277, 63-81[CrossRef][Medline] [Order article via Infotrieve] |
28. |
Elfgang, C.,
Rosorius, O.,
Hofer, L.,
Jaksche, H.,
Hauber, J.,
and Bevec, D.
(1999)
Proc. Natl. Acad. Sci. U. S. A.
96,
6229-6234 |
29. | Hauer, J., Barthe, P., Taylor, S., Parello, J., and Padilla, A. (1999) Protein Sci. 8, 545-553[Abstract] |
30. | Lee, W., Harvey, T., Yin, Y., Yau, P., Litchfield, D., and Arrowsmith, C. (1994) Nat. Struct. Biol. 1, 877-890[Medline] [Order article via Infotrieve] |
31. | Roefs, P., and de Kruif, K. (1994) Eur. J. Biochem. 226, 883-889[Abstract] |
32. |
Chiti, F.,
Webster, P.,
Taddei, N.,
Clark, A.,
Stefani, M.,
Ramponi, G.,
and Dobson, C.
(1999)
Proc. Natl. Acad. Sci. U. S. A.
96,
3590-3594 |
33. | Buccianti, M., Giannoni, E., Chiti, F., Baroni, F., Formigli, L., Zurdo, J., Taddei, N., Ramponi, G., Dobson, C., and Stefani, M. (2002) Nature 416, 507-511[CrossRef][Medline] [Order article via Infotrieve] |
34. |
Lansbury, P.
(1999)
Proc. Natl. Acad. Sci. U. S. A.
96,
3342-3344 |
35. |
Li, S.,
Lam, S.,
Cheng, A.,
and Li, X.
(2000)
Hum. Mol. Genet.
9,
2859-2867 |
36. |
Svennson, M.,
Sabbharwa, H.,
Hakansson, A.,
Mossberg, A.-K.,
Lipniunas, P.,
Leffler, H.,
Svanborg, C.,
and Linse, S.
(1999)
J. Biol. Chem.
274,
6388-6396 |
37. |
Svensson, M.,
Hakansson, A.,
Mossberg, A.-K.,
Linse, S.,
and Svanborg, C.
(2000)
Proc. Natl. Acad. Sci. U. S. A.
97,
4221-4226 |
38. | Noteborn, M., Zhang, Y., and van der Eb, A. (1998) Mutat. Res. 400, 447-255[Medline] [Order article via Infotrieve] |
39. | Dobson, C. (2001) Philos. Trans. R. Soc. Lond.-Biol. Sci. 356, 133-145[CrossRef][Medline] [Order article via Infotrieve] |
40. | Noteborn, M., de Boer, G., van Roozelaar, D., Karreman, C., Kranenburg, O., Vos, J., Jeurissen, S., Hoeben, R., Zantema, A., Koch, G., van Ormondt, H., and van der Eb, A. (1991) J. Virol. 65, 3131-3139[Medline] [Order article via Infotrieve] |
41. | Mi, J., Li, Z., Ni, S., Steinwaerder, D., and Lieber, A. (2001) Hum. Gene Ther. 12, 1343-1352[CrossRef][Medline] [Order article via Infotrieve] |