Nodal Signaling Uses Activin and Transforming Growth Factor-beta Receptor-regulated Smads*

Amit KumarDagger , Vladimir NovoselovDagger §, Anthony J. Celeste, Neil M. Wolfman, Peter ten Dijke||**, and Michael R. KuehnDaggerDagger

From the Dagger  Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892,  Genetics Institute, Cambridge, Massachusetts 02140, and || Division of Cellular Biochemistry, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands

Received for publication, May 30, 2000, and in revised form, October 5, 2000



    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Nodal, a member of the transforming growth factor beta  (TGF-beta ) superfamily, is implicated in many events critical to the early vertebrate embryo, including mesoderm formation, anterior patterning, and left-right axis specification. Here we define the intracellular signaling pathway induced by recombinant nodal protein treatment of P19 embryonal carcinoma cells. Nodal signaling activates pAR3-Lux, a luciferase reporter previously shown to respond specifically to activin and TGF-beta . However, nodal is unable to induce pTlx2-Lux, a reporter specifically responsive to bone morphogenetic proteins. We also demonstrate that nodal induces p(CAGA)12, a reporter previously shown to be specifically activated by Smad3. Expression of a dominant negative Smad2 significantly reduces the level of luciferase reporter activity induced by nodal treatment. Finally, we show that nodal signaling rapidly leads to the phosphorylation of Smad2. These results provide the first direct biochemical evidence that nodal signaling is mediated by both activin-TGF-beta pathway Smads, Smad2 and Smad3. We also show here that the extracellular cripto protein is required for nodal signaling, making it distinct from activin or TGF-beta signaling.



    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Members of the transforming growth factor beta  (TGF-beta )1 superfamily of intercellular signaling factors regulate cell fate and behavior during development and in the adult (1). The three major subgroups based on sequence similarity are the TGF-beta s, activins and inhibins, and bone morphogenetic proteins (BMPs; Ref. 1). Nodal and related factors form a separate subgroup and are implicated in many events critical to the early vertebrate embryo, including mesoderm formation, anterior patterning, and left-right axis specification (2).

Signaling by TGF-beta and related ligands uses two types of receptors, type I and type II transmembrane serine-threonine kinases. Ligand binding results in the formation of heteromeric receptor complexes, in which type II receptors phosphorylate type I receptors (1, 3). Downstream signal transduction events are mediated by the intracellular Smad proteins. One class, the receptor-regulated Smads (R-Smads), are directly phosphorylated by activated type I receptors on a C-terminal SSXS motif. Upon phosphorylation, R-Smads form complexes with the co-Smad, Smad4 and then translocate to the nucleus and regulate transcription of target genes. Biochemical and biological studies have established that the R-Smads used by TGF-beta and activin signaling, Smad2 and Smad3, are distinct from those used by BMP signaling, Smad1, Smad5, and Smad8 (1, 3, 4).

The nodal signaling pathway awaits characterization at the biochemical level. However, mutational studies in the mouse and zebrafish and ectopic expression studies in Xenopus and zebrafish suggest that the nodal and activin signaling pathways may share receptors and Smads. Targeted mutations in the mouse Smad2 gene (5-8) and the activin type IB receptor gene (9) and combined mutations of the activin type IIA and IIB receptor genes (10) show gastrulation phenotypes resembling the mouse nodal mutant (11, 12). Nodal and activin have similar mesoderm-inducing capacity in Xenopus embryo explants or in whole embryos (13), whereas dominant negative forms of Smad2 (14) or activin receptors (15-17) disrupt endogenous mesoderm formation. One difference between nodal and activin signaling has been described in zebrafish. There is genetic evidence that the extracellular epidermal growth factor-Cripto, FRL1, Cryptic (EGF-CFC) protein one-eyed pinhead is required for signaling by zebrafish nodal-related factors but not for activin signaling (18). Whether nodal signaling in the mouse also depends on the function of the related EGF-CFC proteins cripto (19) and cryptic (20) has not been established. However, targeted mutations in both genes result in embryonic lethal phenotypes that are suggestive; cripto null embryos exhibit gastrulation defects (21, 22), and the absence of cryptic leads to left-right defects (23, 24).

Earlier studies on nodal function relied on overexpression from transfected or injected expression vectors. Here we describe the production of recombinant nodal protein. The availability of purified nodal protein allows functional characterization of this developmentally important pathway not previously possible. We describe a quantitative assessment of nodal signaling activity in P19 mouse embryonal carcinoma cells, a model system for the early mouse embryo (25). We have determined that P19 cells are competent to respond to nodal signaling and have exploited this capacity and the availability of recombinant nodal protein to investigate the intracellular pathway by which the nodal signal is transduced. In this study we provide direct evidence that nodal signals through activin-TGF-beta pathway-specific Smads but not BMP pathway-specific Smads. In addition, we show that nodal activity depends on the expression of cripto, suggesting a general requirement for EGF-CFC function in vertebrate nodal signaling.


    EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Recombinant Nodal Protein-- The mature region of human nodal protein was expressed in Escherichia coli and refolded essentially as described (26). Nodal dimer was isolated by ion exchange chromatography on S-Sepharose Fast Flow (Amersham Pharmacia Biotech) using a gradient from 0.05 to 1.0 M NaCl in 25 mM NaAc, 30% isopropanol (pH 4.0), followed by reversed phase high-performance liquid chromatography over a 2.0 × 0.46-cm C4 column (Supelco, Bellefonte, PA) with an acetonitrile gradient in 0.1% trifluoroacetic acid. Protein purity was assessed by SDS-polyacrylamide gel electrophoresis, and concentration was determined by amino acid analysis. Protein activity was assessed using the Xenopus animal cap assay. Briefly, animal caps isolated from stage 8-9 Xenopus embryos were incubated overnight at 16 °C with nodal protein in 50 mM NaCl, 1 mM KCl, 0.5 mM MgSO4, 1 mM CaCl2, 2.5 mM HEPES (pH 7.6), 0.1% bovine serum albumin.

Reagents-- Activin A, TGF-beta 1, BMP4, and BMP6 were from R & D systems (Minneapolis, MN). Tissue culture materials and transfection reagents were purchased from Life Technologies. The gradient gel and polyvinylidene difluoride membrane were obtained from Novex (San Diego, CA). Antibodies PS1 (specific to the C-terminal phosphorylated form of Smad1, Smad5, and Smad8) and PS2 (specific to the C-terminal phosphorylated form of Smad2) were described earlier (27, 28). Additional antibodies to the phosphorylated forms of Smad1 and Smad2, as well as antibodies to the unphosphorylated forms of Smad1, Smad2, and Smad3, were from Upstate Biotechnology (Waltham, MA). A Dual light reporter gene assay kit was purchased from Tropix (Bedford, MA).

Cell Culture-- P19 cells were obtained from American Type Culture Collection and were grown as monolayer cultures in high-glucose Dulbecco's modified Eagle's medium (DMEM) without sodium pyruvate (Life Technologies) supplemented with 7.5% fetal bovine serum (FBS, triple 0.1-µm filtered; Hyclone, Logan, UT). Serum was pretreated with dextran-coated charcoal as described (29). Cells were passaged at confluency (approximately every third day) at a ratio of 1:4. HepG2 human hepatoma cells were grown as monolayer cultures in low-glucose Dulbecco's minimal essential medium (Life Technologies) supplemented with 10% FBS. Cultures were passaged at confluency (approximately twice weekly) at a ratio of 1:10 using trypsin-EDTA.

Plasmids-- The luciferase reporter plasmids p(SBE)4 and p(CAGA)12 were described earlier (30, 31). The pAR3-lux and pTlx2-lux reporter plasmids were described earlier (32, 33). The pGL3 basic and pGL3 promoter vectors were obtained from Promega. The pCDNA3 expression vector was obtained from Invitrogen (Carlsbad, CA). The beta -galactosidase reporter pRSV-beta -galactosidase was provided by P. Yen (National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health). A HindIII-NotI fragment containing the full-length cripto cDNA (a gift from M. Shen) was subcloned into the pCDNA3 vector for expression.

Transcriptional Response Assay-- The day before transfection, P19 cells were plated in 24-well plates at 50,000 cells/well in 0.5 ml of DMEM containing 7.5% FBS and were typically 50-60% confluent at the time of transfection. Plasmid DNA (typically 0.5 µg of total including 0.25 µg of luciferase reporter and 5 ng of pRSV-beta -galactosidase) was mixed with 2 µl of LipofectAMINE Plus reagent (Life Technologies) in 25 µl of DMEM and incubated at room temperature. After 15 min, 25 µl of media containing 1.5 µl of LipofectAMINE was added and incubated for another 30 min. Cells were washed once with DMEM (without FBS), and 200 µl of DMEM was added to each well. 50 µl of the transfection mixture was added to each well, and after 2 h, another 250 µl of DMEM containing 15% FBS was added to each well and incubated overnight (14-18 h). The next day, cells were washed once with DMEM, and fresh media were added. After overnight incubation, media were replaced with serum-free DMEM containing 0.1% bovine serum albumin with or without factors (activin, BMPs, TGF-beta , or nodal) as indicated. The cells were harvested for luciferase assay after 16-20 h of incubation. Similar starting cell numbers and transfection conditions were used for HepG2 cells. Transfections were carried out with 0.5 µg of total DNA (0.25 µg of reporter and 0.25 µg of cripto expression vector). In control reactions, 0.25 µg of pCDNA3.0 was used instead of cripto. HepG2 cells were treated with the same concentrations of ligands as described for P19 cells.

Luciferase and beta -Galactosidase Assay-- Luciferase and beta -galactosidase activity were measured using the Dual light chemiluminescent reporter gene assay kit (Tropix; Applied Biosystems, Foster City, CA) as specified by the manufacturer, in an EG&G Berthold Lumat LB 9507 luminometer. Briefly, cells were washed once with phosphate-buffered saline. After addition of 120 µl of lysis buffer, cells were scraped and centrifuged (4 °C, 12,000 × g, 5 min). 10 µl of the supernatant was added to 25 µl of buffer A, followed by 100 µl of buffer B. After a delay of 2 s, the luciferase signal was measured for 5 s. beta -Galactosidase activity was measured 60 min after measuring luciferase activity. All assays were performed in duplicate. Luciferase activity was normalized to beta -galactosidase activity.

Immunoblot Analysis-- P19 cells were plated in six-well plates (2 × 105 cells/well) in 2 ml of media on day 0. On day 2, media were removed and replaced with DMEM containing 0.1% bovine serum albumin for 3 h. Nodal, activin, or BMP was then added in 0.1% bovine serum albumin for varying times. Cells were washed with ice-cold phosphate-buffered saline, and 200 µl of 1× gel-loading dye was added to each well. Cell lysates were collected, sonicated for 15 s, and heated for 3 min at 95 °C. Samples were fractionated by SDS-polyacrylamide gel electrophoresis using a 10-20% gradient acrylamide gel. Transfer to the polyvinylidene difluoride membrane and processing were as described previously (34, 35). Briefly, membranes were incubated in TNT 20 (20 mM Tris-HCl (pH 7.6), 137 mM NaCl, 0.1% v/v Tween 20) with 5% w/v nonfat dried milk for 1 h, washed in TNT 20 (3-5 min), and incubated with primary antibody (1:1000) in TNT 20 containing 1% milk. This was done for 1 h at room temperature for non-phospho-specific antibodies and overnight at 4 °C for phospho-specific antibodies. After further washing in TNT 20, membranes were incubated for 1 h with horseradish peroxidase-linked anti-IgG secondary antibody (Pierce; 1:5000), and immunoreactive proteins were detected using SuperSignal chemiluminescent substrate (Pierce).


    RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

P19 Embryonal Carcinoma Cells Are Responsive to Recombinant Nodal Protein-- Unpublished work from our laboratory has shown that P19 embryonal carcinoma cells stably transfected with a nodal expression vector can differentiate into mesoderm without chemical induction.2 These results suggested that P19 cells are responsive to nodal and may provide an appropriate model system to study nodal function in the early mouse embryo. To confirm these results and establish a reporter assay to dissect the nodal signaling pathway, we carried out transient transfection of undifferentiated P19 cells using the p(SBE)4 luciferase reporter. p(SBE)4 has been shown to be a general reporter for the TGF-beta superfamily in HepG2 cells, responding to TGF-beta itself, activin, and BMP2 (30). Therefore, it was a good candidate reporter to confirm nodal signaling in P19 cells without knowing any details of the pathway. We first determined that p(SBE)4 is induced by TGF-beta , activin, and BMP2 in P19 cells. As shown in Fig. 1A, activin (20 ng/ml), BMP4 (40 ng/ml), and TGF-beta 1 (1 ng/ml) increased p(SBE)4 reporter activity by 9 ± 1.8-, 4 ± 2.3-, and 3 ± 0.4-fold (mean ± S.D.), respectively. These results established that p(SBE)4 is a general reporter for both TGF-beta -activin and BMP pathway-specific R-Smad activation in P19 cells, as in HepG2 cells.



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Fig. 1.   Recombinant nodal protein activates the p(SBE)4 reporter in P19 cells. Luciferase activity in the absence of any factor is taken as 100%. A, treatment with activin (20 ng/ml), BMP4 (40 ng/ml), or TGF-beta (1 ng/ml) leads to a significant increase in p(SBE)4 activity in P19 cells. The mean + S.D. for six experiments is plotted. B, treatment of P19 cells with increasing concentrations of nodal (0.25-4 µg/ml) leads to an increase in p(SBE)4 activity. The mean + S.D. of two experiments is plotted.

To analyze nodal signaling, we used recombinant human nodal protein, produced in bacteria and refolded in vitro. Nodal protein activity was assayed using Xenopus embryo explants. Animal caps treated with nodal protein (at 5 µg/ml) or activin (at 2.5 or 25 ng/ml) underwent elongations characteristic of mesoderm formation (data not shown), confirming the bioactivity of the recombinant protein preparation. We then evaluated p(SBE)4 activation by recombinant nodal treatment of P19 cells. As shown in Fig. 1B, nodal induced p(SBE)4 reporter activity in a dose-dependent manner with a 14-fold increase at 4 µg/ml. These studies confirmed that P19 cells are competent to respond to nodal and provide an experimental system for defining the intracellular signaling pathway. In further studies, we used 2 µg/ml nodal, because this amount still gave a significant level of induction within the range of activation induced by the other ligands.

Nodal Signaling Requires EGF-CFC Function-- The specific activity of recombinant nodal protein was low compared with the other ligands tested, perhaps because of a significant amount of inactive protein in the high-performance liquid chromatography-purified fraction. The requirement of using such large amounts of nodal protein raised the question of specificity: is the signaling truly representative of endogenous nodal signaling, or is it caused by low-affinity binding to nonphysiological receptors? To address this question, we analyzed nodal signaling in HepG2 cells. As shown in Fig. 2A, activin, BMP4, and TGF-beta induced p(SBE)4 activity in transfected HepG2 cells by 8.5 ± 1.4-, 8.8 ± 1.4-, and 5.8 ± 0.1-fold, respectively. However, we saw no activation of p(SBE)4 by nodal at 2 µg/ml, the same amount that gave a significant level of induction in P19 cells. This result indicated that our preparation of recombinant nodal protein, even at this concentration, does not cross-react with the TGF-beta , activin, and BMP receptors clearly present on HepG2 cells.



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Fig. 2.   Nodal activates p(SBE)4 in HepG2 cells only when Cripto is coexpressed. A, treatment of HepG2 cells with nodal has no effect on p(SBE)4 activity, whereas activin (20 ng/ml), BMP4 (40 ng/ml), or TGF-beta (1 ng/ml) leads to a significant increase. The mean + S.D. for three separate experiments is shown. B, HepG2 cells transfected with a Cripto expression vector show a significant increase in p(SBE)4 activity when treated with nodal (2 µg/ml). The mean + S.D. for three separate experiments is shown.

The nonresponsiveness of HepG2 cells to nodal treatment indicates these cells lack a component specific to the nodal signaling pathway. Based on results in zebrafish suggesting that nodal signaling requires EGF-CFC function (18) and unpublished data showing that HepG2 cells lack expression of full-length cripto,3 we asked whether transfection of a cripto expression vector could rescue nodal signaling in HepG2 cells. As shown in Fig. 2B, nodal treatment of HepG2 cells expressing cripto led to a significant increase in p(SBE)4 reporter activity (4.92 ± 0.95-fold) compared with controls. This result provides further support that the recombinant protein is acting physiologically and suggests that EGF-CFC activity is a general requirement for vertebrate nodal signaling.

Nodal Induces a TGF-beta and Activin Response but Not a BMP Response-- To determine whether intracellular nodal signals are mediated through the TGF-beta -activin or BMP pathway, or both, we used two reporters that differentiate between these pathways. pAR3-lux contains an activin response element from the Xenopus Mix.2 gene and is activin and TGF-beta inducible in Xenopus embryos (36) and in HepG2 cells (32). The pTlx2-lux reporter contains a BMP-responsive element and is BMP2, BMP7, and Smad1 inducible in P19 cells (33, 37). pTlx2-lux has also been shown to be induced by constitutively active forms of the BMP-specific type I receptors activin receptor-like kinase 2 and activin receptor-like kinase 6 but not by a constitutively active activin receptor-like kinase 4 receptor, an activin-specific type I receptor (37). We transiently transfected P19 cells with pAR3-lux and pTlx2-lux reporters and treated them with nodal (2 µg/ml), activin (20 ng/ml), BMP4 (40 ng/ml), and BMP6 (40 ng/ml). As shown in Fig. 3A, activin treatment of cells transiently transfected with pAR3-lux resulted in a 4.8 ± 0.9-fold induction of luciferase activity, consistent with previous reports (37). When cells were treated with nodal, pAR3-lux activation increased by 3 ± 0.4-fold. Neither BMP4 nor BMP6 had any significant effect on the activity of pAR3-lux. As shown in Fig. 3B, BMP4 and BMP6 induced pTlx2-lux by 1.8 ± 0.12- and 2.2 ± 0.26-fold, respectively. As expected, we did not see any induction by activin. Nodal also had no detectable effect on the Tlx2 promoter. Taken together, our results suggest that nodal uses only the TGF-beta -activin signaling pathway in P19 cells.



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Fig. 3.   Nodal activates pAR3-lux but not pTlx-2-lux in P19 cells. Luciferase activity in the absence of any factor is taken as 100%. A, the pAR3-lux reporter responds to activin (20 ng/ml) and nodal (2 µg/ml) but not to BMP4 or BMP6 (each 40 ng/ml). B, pTlx-2-lux only responds to BMPs and not to either activin or nodal. The mean + S.D. for three experiments is plotted.

Nodal Signaling Is Mediated by Smad3-- The response of the activin response element to activin and TGF-beta treatment was previously shown to be mediated by either Smad2 or Smad3 (38-40). To determine whether nodal signals through Smad2 or Smad3, or both, in P19 cells, we first used the p(CAGA)12 luciferase reporter. This reporter is activated only in response to activin and TGF-beta signaling and only through Smad3 and not Smad2 in HepG2 and Mv1Lu cells (31). As shown in Fig. 4, activin and TGF-beta induced p(CAGA)12 by 9.4 ± 3.1- and 6.5 ± 3.1-fold respectively in P19 cells, whereas BMP4 had no significant effect. Treatment with nodal led to a 6.2 ± 1.5-fold increase in p(CAGA)12 activity. These results indicate that Smad3 can mediate nodal signaling in P19 cells.



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Fig. 4.   Nodal activates the Smad3-specific luciferase reporter p(CAGA)12 in P19 cells. Luciferase activity in the absence of any factor is taken as 100%. Activin (20 ng/ml), nodal (2 µg/ml), and TGF-beta 1 (1 ng/ml) lead to a significant increase in p(CAGA)12 reporter activity, whereas BMP4 (40 ng/ml) does not. The mean + S.D. for five experiments is plotted.

Nodal Signaling Is Reduced by a Dominant Negative Smad2-- To determine the involvement of Smad2 in nodal signaling, we first used a dominant negative approach. Previous work has shown that C-terminal serines in Smad2 are phosphorylated by the TGF-beta receptor, and mutating these serines to alanines prevents Smad2 phosphorylation and its nuclear accumulation (41). These mutant forms of Smad2 can still associate with the receptor, however, and can act as dominant negatives. We used the dominant negative Smad2 (3S-A) to test whether it affected nodal-mediated p(SBE)4 activation. P19 cells were transfected with p(SBE)4 either alone or together with Smad2 (3S-A), and luciferase activity was measured after treatment with nodal or activin. The results are shown in Fig. 5A. In the absence of Smad2 (3S-A), p(SBE)4 activity was increased 6- and 9-fold by nodal and activin, respectively, which was taken as 100% activity. In the presence of Smad2 (3S-A), we observed only 3.96- and 6.75-fold induction by nodal and activin, respectively, a 34 and 25% reduction. We repeated these experiments using the activin- and TGF-beta -specific reporter pAR3-lux. As shown in Fig. 5B, expression of the dominant negative Smad2 led to a 40% reduction of nodal-induced pAR3-lux activity. These results indicate that nodal also can use Smad2 for intracellular signaling in P19 cells.



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Fig. 5.   Nodal signaling is decreased in P19 cells expressing dominant negative Smad2. Cells were cotransfected with 0.2 µg p(SBE)4 or pAR3-lux and 0.01 µg of Smad2 (3S-A). The level of luciferase activity after either nodal or activin treatment in the absence of Smad2 (3S-A) is taken as 100%. A, in Smad2 (3S-A)-transfected cells treated with nodal (2 µg/ml) or activin (20 ng/ml), there is a significant reduction of p(SBE)4 activity. The mean + S.D. for three experiments is shown. B, cells expressing Smad2 (3S-A) treated with nodal (2 µg/ml) or activin (20 ng/ml) show a similar reduction of pAR3-lux activity.

Nodal Signaling Results in Phosphorylation of Smad2-- As a second approach to determine Smad2 involvement in nodal signaling, we examined Smad2 phosphorylation after nodal treatment of P19 cells. These studies used antisera specific for the C-terminal phosphorylated form of Smad2. These antibodies do not recognize the unphosphorylated (nonactivated) forms of these Smads. P19 cells were treated with nodal (2 µg/ml), activin (20 ng/ml), or BMP4 (40 ng/ml) for various times, and cell lysates were analyzed by Western blotting. As shown in Fig. 6A, nodal activated Smad2 within 15 min, and phosphorylation was found for at least 4 h before returning to basal levels by 24 h. Activin treatment also led to Smad2 phosphorylation in P19 cells. However, peak levels for activin stimulation were seen at 1 h with a return to basal level by 4 h.



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Fig. 6.   Nodal signaling leads to phosphorylation of Smad2 but not BMP R-Smads in P19 cells. A, immunoblots of nodal- and activin-treated samples. Top panel, samples analyzed with the PS2 antibody specific to phosphorylated Smad2. Both ligands cause rapid phosphorylation of Smad2 but with different kinetics. Bottom panel, same samples analyzed with anti-Smad2 antibody. B, immunoblots of BMP4- and nodal-treated samples. Top panel, samples analyzed with the PS1 antibody specific to phosphorylated Smads 1, 5, and 8. BMP4 treatment results in rapid phosphorylation, whereas nodal has no effect. Bottom panel, same samples analyzed with anti-Smad1 antibody. The results shown are representative of three separate experiments.

To confirm that nodal signaling does not go through BMP R-Smads, we used antisera specific for the C-terminal phosphorylated forms of Smads 1, 5, and 8. As shown in Fig. 6B, BMP4 treatment led to rapid phosphorylation of these BMP R-Smads, which returned to basal levels by 24 h. However, treatment with nodal had no effect. These results provide direct evidence that nodal signals through Smad2 and conclusively show that BMP R-Smads are not used.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

We have obtained several lines of evidence indicating that nodal activity in P19 cells is regulated through the activin-TGF-beta pathway mediated by Smad2 and Smad3 and not through BMP-regulated Smads. First, nodal induces pAR3-lux, which was previously shown to be specifically responsive to TGF-beta and activin (37). Nodal does not activate pTlx2-lux, which has been shown to be strictly BMP inducible (33, 37). Second, nodal activates the p(CAGA)12 reporter, which was previously shown to be activated specifically by Smad3 (31). Third, nodal activation of either p(SBE)4 or pAR3-lux is reduced significantly by expression of a dominant negative form of Smad2. Fourth, nodal treatment of P19 cells rapidly induces the phosphorylation of Smad2 but not BMP R-Smads.

The involvement of Smad2 in nodal signaling in P19 cells suggests that a similar pathway exists in the intact embryo. Several different targeted mutations of Smad2 in the mouse germ line have been reported (5-8). The studies by Nomura and Li (7) and Weinstein et al. (6) have described gastrulation defects similar to those of the nodal null mutant, which lacks mesoderm formation (11, 12, 42). The similarity of these phenotypes is consistent with nodal signaling using Smad2 in the gastrulating embryo. Nomura and Li (7) also reported that embryos heterozygous for both the Smad2 and nodal mutations have phenotypic defects in left-right axis development and anterior patterning. Heyer et al. (8) reported similar phenotypes in Smad2 mutant embryos rescued through gastrulation. These latter phenotypes are similar to that produced by a hypomorphic nodal mutation,4 indicating that nodal signaling uses Smad2 in developmental processes other than mesoderm formation. However, the Smad2 knockouts reported by Waldrip et al. (5) and Heyer et al. (8) showed transient mesoderm formation, complicating the conclusion that nodal uses Smad2 in the induction of mesoderm in the embryo. Our conclusive demonstration that nodal signals through Smad2 in P19 cells will aid in the interpretation of these different Smad2 knockout phenotypes.

We have shown that nodal signaling also can use Smad3 in P19 cells. However, targeted mutation of Smad3 in the mouse does not lead to early developmental defects (43-45), suggesting that Smad3 is not involved in nodal signaling in the embryo or that Smad2 can compensate for the loss of Smad3. Interestingly, embryos heterozygous for both Smad2 and Smad3 can display left-right abnormalities.5 This finding suggests that nodal signaling may indeed involve both Smad2 and Smad3 in the embryo.

Although we show here that nodal uses an activin-like intracellular signaling pathway, we have also found some major differences between nodal and activin signaling. We have found that human HepG2 cells are not responsive to human nodal protein unless transfected with an expression vector encoding the extracellular EGF-CFC protein cripto. Previous genetic studies demonstrated a requirement for the zebrafish EGF-CFC protein one-eyed pinhead in nodal-related signaling in the zebrafish but not for activin signaling (18). A recent study showed that injection of a mouse nodal expression vector into Xenopus embryos resulted in induction of a luciferase reporter carrying an enhancer element from the nodal gene, but at a significantly lower level than that obtained with an activin expression vector. However, co-injection of nodal and cripto expression vectors led to similarly high levels as with the activin expression vector (46). Together these data suggest that there is a general requirement for EGF-CFC protein function in vertebrate nodal signaling.

Another difference between activin and nodal signaling is in the persistence of Smad2 phosphorylation occurring in response to these two factors. We found that during continuous activin treatment of P19 cells the levels of activated Smad2 peaked at 1 h and then declined. This is similar to what was found for Smad2 activated by continuous TGF-beta treatment of HaCaT cells (47). However, Smad2 phosphorylation induced by continuous nodal treatment of P19 cells was sustained for much longer. This difference may be attributable to differences at the level of receptor-mediated phosphorylation of Smad2 or at the level of Smad2 turnover. Lo and Massagué (47) showed that TGF-beta activation of Smad2 and translocation to the nucleus leads to multiubiquitination of Smad2 and subsequent degradation by the proteasome. Smad2 activated by nodal may persist longer, because it may be ubiquitinated with different kinetics than Smad2 activated by TGF-beta or activin. Alternatively, the receptor complex brought together by nodal may be more stable than that formed by TGF-beta or activin receptor binding, perhaps through the action of extracellular EGF-CFC proteins. Increased receptor complex stability might result in more sustained activation of Smad2.

In conclusion, we have shown that nodal signaling in P19 cells is mediated intracellularly through the TGF-beta -activin pathway, providing important biochemical evidence that nodal signaling in the embryo uses Smad2 and Smad3.


    ACKNOWLEDGEMENTS

We thank Mark deCaestecar for the Smad2 dominant negative construct, Jeff Wrana for the pAR3-lux and pTlx2-lux reporters, Michael Shen for the cripto cDNA, Ester Piek for many useful suggestions, and Anita Roberts and Linda A. Lowe for critical reading of the manuscript.


    FOOTNOTES

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Present address: Dept. of Embryology, Moscow State University, Moscow 119899, Russia.

** Supported by the Dutch Organization for Scientific Research (Project 809.67.021).

Dagger Dagger To whom correspondence should be addressed: Experimental Immunology Branch, NCI, National Institutes of Health, Bldg. 10, Rm. 4B-36, 10 Center Dr., Bethesda, MD 20892-1360. Tel.: 301-435-6476; Fax: 301-496-0887; E-mail: mkuehn@mail.nih.gov.

Published, JBC Papers in Press, October 9, 2000, DOI 10.1074/jbc.M004649200

2 V. Novoselov and M. R. Kuehn, unpublished data.

3 D. Salomon, personal communication.

4 L. A. Lowe, S. Yamada, and M. R. Kuehn, submitted for publication.

5 M. Weinstein and C. X. Deng, personal communication.


    ABBREVIATIONS

The abbreviations used are: TGF, transforming growth factor; BMP, bone morphogenetic protein; R-Smad, receptor-regulated Smad; DMEM, Dulbecco's modified Eagle's medium; FBS, fetal bovine serum; SBE, Smad binding element; EGF-CFC, epidermal growth factor-Cripto, FRL1, Cryptic.


    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES


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