ACCELERATED PUBLICATION
Activation of Myosin Light Chain Kinase Requires Translocation of Bound Calmodulin*

Joanna K. KruegerDagger §, Stephen C. GallagherDagger , Gang Zhi, Ramaz Geguchadze, Anthony Persechini||, James T. Stull, and Jill TrewhellaDagger **

From the Dagger  Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545; the  Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Texas 75390; and the || Division of Molecular Biology and Biochemistry, University of Missouri-Kansas City, Kansas City, Missouri 64110

Received for publication, December 4, 2000



    ABSTRACT
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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

A novel translocation step is inferred from structural studies of the interactions between the intracellular calcium receptor protein calmodulin (CaM) and one of its regulatory targets. A mutant of CaM missing residues 2-8 (Delta NCaM) binds skeletal muscle myosin light chain kinase with high affinity but fails to activate catalysis. Small angle x-ray scattering data reveal that Delta NCaM occupies a position near the catalytic cleft in its complex with the kinase, whereas the native protein translocates to a position near the C-terminal end of the catalytic core. Thus, CaM residues 2-8 appear to facilitate movement of bound CaM away from the vicinity of the catalytic cleft.



    INTRODUCTION
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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

A wide variety of Ca2+-dependent cellular processes are regulated by the ubiquitous Ca2+-binding protein CaM,1 which interacts with a diverse array of target proteins, including a number of protein kinases (1). Ca2+/CaM-dependent activation of MLCK has provided a number of important insights into the molecular mechanisms underlying CaM-dependent kinase activation. All isoforms of MLCK include a conserved protein kinase catalytic core (2) that is followed immediately by a regulatory segment consisting of autoinhibitory and CaM-binding sequences. The catalytic core is believed to have the distinctive bilobal structure characterized first by the crystal structure of the cAMP-dependent protein kinase (3). A cleft between the lobes defines the catalytic site for substrate phosphorylation. In its inhibited state, the regulatory segment of MLCK maintains numerous contacts with the larger lobe of the catalytic core, including a few sites of contact in the catalytic cleft itself, thus preventing myosin light chain binding and catalysis (4-6). Our previous scattering studies of a catalytically competent truncation mutant of skMLCK (7) support the idea of the autoinhibitory release mechanism (6) for kinase activation in which CaM binding induces a significant movement of the regulatory segment away from the surface of the catalytic core necessary for binding and phosphorylating myosin regulatory light chain. More recently, we showed that substrate binding induces a closure of the catalytic cleft and a reorientation of CaM that allows for more extensive surface contact between the catalytic core and the N-terminal sequence of CaM (8).

CaM has two globular Ca2+-binding lobes connected by a central helix (9) that functions as a "flexible tether" (10, 11). The concept of the flexible tether function obtained its strongest support from structural studies of CaM complexed with isolated peptides containing the core CaM-binding sequences from kinases that show CaM undergoes a dramatic conformational collapse (11-15). Flexibility in the central helix allows the two lobes of CaM to come together, enfolding the helical-configured target peptides. A similar conformational collapse of CaM was observed in scattering studies of its complex with a catalytically competent skMLCK (7, 8). The known structures of all the CaM·peptide complexes show little or no interaction between the N-terminal leader sequence of CaM (residues 1-8, ADQLTEEQ) and the bound peptides. Nevertheless, deletion of residues 2-8 results in a CaM mutant (Delta NCaM) that binds with high affinity but completely fails to activate skMLCK (16). The mutant protein also has a reduced capacity to activate the smooth muscle form of the kinase but can fully activate neuronal nitric-oxide synthase activity, suggesting that the N-terminal leader sequence plays a specialized role in a subset of CaM-target complexes.

We present here the results of scattering experiments on Delta NCaM and native CaM in their respective complexes with a catalytically competent skMLCK (17) that reveal the dramatic structural basis for the inability of Delta NCaM to activate skMLCK catalysis.


    MATERIALS AND METHODS
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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Sample Preparation-- The Delta NCaM was expressed in Escherichia coli and purified as described in (16). CaM, skMLCK, and the complexes were prepared as described in (8). All samples contained excess Ca2+ so that the CaMs were Ca2+ saturated. Buffer conditions were 25 mM MOPS, pH 8.0, 25 mM KCl, 5 mM magnesium acetate, 1 mM dithiothreitol, and 2.5% glycerol.

Scattering Data Acquisition-- Scattering data were acquired using the x-ray instruments at Los Alamos (18, 19) and reduced to I(Q) versus Q as described in (18). Q is (4pi sintheta )/lambda where theta  is half the scattering angle and lambda  is the wavelength of the scattered radiation. Structural information is derived by calculating the inverse Fourier transform of I(Q), which yields P(r), the probable frequency distribution of interatomic vectors, r, within the scattering molecule (calculated using the program GNOM (20)). P(r) goes to zero at the maximum linear dimension, dmax, of the scattering particle and its zeroth and second moments give forward scatter, I0 and radius of gyration, Rg, respectively. The forward scatter, I0, is directly proportional to the product of the protein concentration, c, (in mg/ml) and the molecular weight of the scattering molecule, M. I0 is thus very sensitive to changes in the size of the scattering particle because of complex formation or aggregation.

Modeling the Scattering Data-- Model I(Q) and P(r) profiles for the two ellipsoid models were calculated using SASMODEL (21), which uses a rapid Monte Carlo integration routine to generate model P(r) functions (18). The P(r) and I(Q) profiles based on models using crystal structure coordinates were calculated using PRPDB (19). Both SASMODEL and PRPDB calculate I(Q) as the Fourier transformation of P(r). P(r) and I(Q) functions are then compared with the experimental data by calculating chi 2 for the experimental and model I(Q) profiles,


&khgr;<SUP>2</SUP>=(1/N) <LIM><OP>∑</OP></LIM> (I<SUB><UP>obs</UP></SUB>−I<SUB><UP>calc</UP></SUB>)<SUP>2</SUP>/&sfgr;<SUP>2</SUP> (Eq. 1)
where the summation is over N, the number of Q values for which I(Q) is measured, Iobs is the observed intensity at each Q value, sigma  is the S.D. for each Iobs, and Icalc is the calculated intensity at each Q value for the model being tested. A value of 1.0 is a perfect fit with the observed data within the errors.


    RESULTS
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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Scattering Data-- The scattering data (Fig. 1A) for free Delta NCaM indicate it has a slightly more compact structure than native CaM (Table I). Fig. 1C compares the vector length distribution functions, P(r), for the two proteins. Based on calculations of model P(r) functions using the crystal structure for CaM (not shown), this difference can be accounted for solely by the deletion of the N-terminal residues in the mutant protein. The scattering data thus indicate that Delta NCaM has a two lobed structure, similar to native CaM. In contrast, the solution structures of the Delta NCaM and CaM complexes with skMLCK differ dramatically. The experiments on the complexes were performed with and without substrates; a peptide containing the phosphorylation sequence from myosin regulatory light chain (pRLC, sequence KKRAARATSNVFS) and a nonhydrolyzable analog of adenosine-triphosphate (AMPPNP). The same differences were observed between the native CaM and Delta NCaM complexes in the presence or absence of substrates. We used data collected in the presence of the substrates for our analyses, because the native CaM·skMLCK complex is less prone to aggregation under these conditions. The I0 values indicate that the protein solutions contain monodisperse particles with the molecular weights expected for 1:1 complexes (Table I). As we have reported previously (7, 8), the P(r) function and Rg value for the CaM·skMLCK complex (Fig. 1D) indicates an elongated structure (Table I). In contrast, data for the Delta NCaM·skMLCK complex indicate a more symmetrical structure, evident in its significantly smaller dmax and Rg values (Table I).



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Fig. 1.   Scattering data and P(r) functions for CaM, Delta NCaM, and their complexes with skMLCK. A, I(Q) versus Q for CaM () and Delta NCaM (open circle ). B, the CaM·skMLCK (black-square) and Delta NCaM·skMLCK () complexes with substrates AMPPNP and myosin light chain peptide are presented. Data are binned and shown offset on an arbitrary log scale. Solid and dotted lines overlaying each data set in B represent I(Q) versus Q calculated for the best-fit uniform density and higher resolution models, respectively (see Fig. 2). Data were fit from Q = 0.02 to 0.20 Å-1. C, P(r) functions scaled by their respective molecular weights for CaM () and Delta NCaM (open circle ). D, the CaM·skMLCK (black-square) and Delta NCaM·skMLCK () complexes with the solid lines showing the P(r) calculated for the best-fit high resolution model shown in Fig. 2.


                              
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Table I
Structural parameters for Delta NCaM, CaM, and their respective complexes with skMLCK in the presence of substrates (AMPPNP and myosin light chain peptide)

Two-Ellipsoid Model of the Complexes Reveals Sizes and Dispositions of Components-- To gain further insights to the relative sizes and dispositions of CaM or Delta NCaM and skMLCK, we simulated x-ray scattering data using SASMODEL (see "Materials and Methods"). Each complex was modeled as two uniform density ellipsoids, representing CaM or Delta NCaM and skMLCK. During this procedure the ellipsoids were required to be in contact, and their dimensions were loosely constrained to be in a range consistent with the dimensions for CaM and skMLCK determined in previous scattering studies (8). Specifically, the ellipsoid semi-axis lengths were constrained to be in the ranges 38-48 Å, 23-35 Å, and 16-22 Å for skMLCK and 22-28 Å, 18-24 Å, and 14-19 Å for CaM. For the Delta NCaM complex, we also allowed the bound Delta NCaM to assume the more elongated shape associated with its uncomplexed state, to test the possibility that the Delta NCaM might not collapse upon binding. In each calculation, ~104 different models were tested, with randomly chosen ellipsoid dimensions, relative orientations, and contact points. The best-fit models are depicted in Fig. 2 (green crosses), and their respective fits (chi 2) and structural parameters are given in Table II. As expected, the model for the native CaM·skMLCK complex is similar to the one proposed previously based on neutron contrast variation data for the complex with deuterated CaM (8). Contrast variation allows us to readily distinguish CaM and skMLCK in the complex, and we therefore were able to unequivocally assign the smaller ellipsoid to the CaM. The model for the Delta NCaM·skMLCK complex indicates individual ellipsoid dimensions similar to those for the native CaM·skMLCK complex. We therefore conclude that, like native CaM, Delta NCaM collapses about the core CaM-binding sequence of the kinase in this complex. A most significant difference between the complexes is that bound Delta NCaM is more centrally located with respect to the long axis of the kinase than the bound native protein.



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Fig. 2.   Best-fit ellipsoid models (pale green dots) for the CaM·skMLCK (bottom) and Delta NCaM·skMLCK (top) complexes superimposed with ribbon representations of the respective higher resolution models. The ribbons for skMLCK and the CaM-binding peptide (MLCK-I) are red and for CaM, cyan. The pale green dots represent random points that fill the ellipsoid shapes and are used to calculate model P(r) functions from which model scattering profiles are generated. The N terminus of CaM in both models is indicated with N.


                              
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Table II
Parameters for the best-fit models to the data and dimensions of the ellipsoids for CaM·skMLCK and Delta NCaM·skMLCK

Higher Resolution Structural Model for the Complex Reveals Position of the Catalytic Cleft with Respect to the CaM-binding Site-- To evaluate how sensitive the scattering data are to the position of CaM or Delta NCaM relative to the catalytic cleft in the kinase, we performed additional calculations using higher resolution structural models. We modeled the catalytic core of skMLCK using the crystal structure of the catalytic subunit of the cAMP-dependent protein kinase, cPKA (Ref. 3, PDB accession number 2cpk) and SwissModel, an automated homology modeling package (22). The cPKA and skMLCK catalytic cores have 29.4% amino acid sequence identity and 61% sequence similarity, making cPKA a good template for homology modeling. For sequences with 30-40% sequence identity, homology modeling can yield structures that give backbone root mean square deviation (RMSD) values from experimentally derived structures as low as 1.5 Å (23). To model bound CaM or Delta NCaM, we used the NMR structure for CaM complexed with a peptide based on the core CaM binding sequence in skMLCK (MLCK-I, Ref. 14, PDB accession number 2bbm), deleting residues 2-8 for the complex with Delta NCaM. The skMLCK model does not account for 32 N-terminal residues and 52 C-terminal residues in the kinase, as they have no corresponding sequences in cPKA. However, of the C-terminal residues, 23 are accounted for by the core CaM-binding sequence. Thus, the structural models for the complexes account for ~90% of the skMLCK sequence.

Simulated scattering data for the Delta NCaM or CaM complexes with skMLCK were generated by positioning the modeled components relative to each other and calculating the expected scattering profiles using PRPDB (see "Materials and Methods"). Two approaches were used. The first was to randomly orient and position the components with respect to each other while loosely constraining the distance between their centers of mass to be no more than 60 Å (22,400 models tested). Using this approach, ideal chi 2 values (chi 2 = 1.00) were obtained for both complexes. In the second approach, the two components were placed in the same arbitrary relative positions, and the CaM·MLCK-I component was systematically moved through every position on a 5 Å grid extending ± 50 Å in each of the x, y, and z directions (9,261 possible x, y, z coordinates tested). The best-fit model identified from the 5 Å grid search was further refined by performing a finer grid search (1Å resolution, ±5 Å in each direction, 1,331 possible x, y, z coordinates tested). In a final refinement step, the two components were randomly rotated about their origins in all directions (125,000 different orientations tested). Using this approach we also were able to obtain chi 2 values of 1.00 for both the CaM·skMLCK and Delta NCaM·skMLCK complexes. The relative positions of skMLCK and CaM based on the random search and the systematic grid search were equivalent, giving RMSD main chain deviations between the differently derived models of 0.22 and 0.26 Å for the CaM·skMLCK and Delta NCaM·skMLCK complexes, respectively. Fig. 2 shows the backbone traces of the best-fit higher resolution model for each complex, overlaid with its corresponding best-fit ellipsoid model. For both complexes, there is excellent agreement in the positions of each component determined using the two modeling approaches. Similar to our previous analysis based on neutron scattering data, the higher resolution model for the skMLCK component does not completely fill the ellipsoid dimensions calculated using the x-ray scattering data, which is to be expected given the N- and C-terminal sequence segments omitted from the homology model.

The best-fits to the scattering data obtained using the higher resolution structural models predict a specific orientation of the kinase catalytic cleft with respect to bound CaM or Delta NCaM (see Fig. 2). To evaluate the sensitivity of the scattering data to the relative orientations of the CaM and kinase components, we performed additional calculations in which they were rotated about the three perpendicular axes defined by their associated best-fit ellipsoids. The rotations allowed us to test different orientations of the kinase and CaM structures while leaving the ellipsoid envelopes unperturbed. At each rotation angle (sampled in 30° steps) a translation search of ± 5 Å in the x, y, and z directions with a 1 Å step size was performed to optimize the translation for each rotation angle. The best-fit obtained after this translational search was considered the best one for that rotation angle. Fig. 3 presents a plot of the best chi 2 values obtained for the calculated scattering profiles and the experimental data at each rotation angle. All rotations of the kinase or CaM components away from their positions in the starting best-fit models result in significantly poorer fits for both complexes. The sensitivity to rotation angle is greatest for the kinase component, which shows the narrowest and deepest minimum with respect to this parameter, presumably because of its more irregular shape. In contrast, fits for the CaM and Delta NCaM structures go through shallower and broader minima in chi 2 as a function of rotation angle. In the best-fit models for both complexes, the CaM-binding peptide of MLCK is positioned with its N terminus pointing toward the kinase, which allows it to be connected with this sequence. In addition, the N-terminal helix of CaM in the native complex is in contact with the kinase, as was observed for the model derived from the neutron contrast variation data (8). The I(Q) functions derived from the best-fit models for each complex are compared with the experimental I(Q) and P(r) data in Fig. 1, B and D, respectively.



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Fig. 3.   Plots of chi 2 values calculated for the experimental and model I(Q) profiles as a function of the rotation angle for skMLCK or CaM components away from the orientations obtained in the best-fit high resolution models. A, chi 2 values calculated for models obtained by rotating the kinase structure away from the orientation obtained for the final best-fit model for the CaM·skMLCK complex. Rotations are about the orthogonal axes; x (black-square), y (black-triangle), and z (black-diamond ). B, as in A but for rotations of CaM. C-D, as in A-B but for rotation of the skMLCK and Delta NCaM components of the Delta NCaM·skMLCK complex. The principal axes for the rotations are defined using a right-handed system, assigning the y axis to the longest dimension for each component.

Because of the potential importance of the changes in CaM residues 1-8 in the activation mechanism (16), three peptides were synthesized for competitive binding studies; the native sequence, the native sequence with Glu residues substituted for Ala, and a scrambled native sequence. None of these peptides affected the catalysis or CaM activation properties at 1 mM (data not shown). This result suggests that the N-terminal sequence is not simply functioning as a latch to hold bound CaM in the requisite position away from the catalytic site.


    DISCUSSION
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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Our results indicate that removal of the N-terminal leader sequence from CaM results in a complex with skMLCK in which the mutant protein blocks the catalytic cleft. This result is in stark contrast with the complex with native CaM, in which bound CaM appears to translocate so that the catalytic cleft is exposed, and the N-terminal helix of CaM interacts with the C-terminal portion of the catalytic core. The higher resolution modeling of the native CaM·skMLCK complex also resolves the ambiguity that remained in the interpretation of the original two ellipsoid modeling of the neutron data regarding which side of the kinase, with respect to the catalytic cleft, is the CaM-binding site (8). In both native and mutant complexes CaM adopts a collapsed structure in which both lobes likely interact with the core CaM-binding sequence in the kinase. These results are consistent with the fact that although native CaM and Delta NCaM bind skMLCK with equal affinities only the native protein is capable of activating the kinase catalytic function (16). The inability of Delta NCaM to activate the kinase would appear to be due at least in part to its blocking of the catalytic cleft. In this position, it may also fail to disrupt autoinhibitory interactions with the catalytic core. In contrast, a translocation of bound native CaM exposes the catalytic cleft and likely also disrupts interactions between the catalytic core and the autoinhibitory sequence, thus allowing catalysis to proceed. The precise role of the N-terminal leader sequence in facilitating translocation of bound CaM is not fully resolved. The simplest interpretation is that CaM residues 2-8 facilitate displacement of an autoinhibitory region flanking the core CaM-binding sequence in the kinase, allowing translocation of bound CaM to a position at the end of the C-terminal lobe of the enzyme, thereby allowing substrate binding to the exposed catalytic cleft.


    FOOTNOTES

* This work was performed under the auspices of the Department of Energy (DOE) under contract to the University of California and was supported by DOE/Office of Biological and Environmental Research Project KP1101010 (to J. T.) and by National Institutes of Health Grants GM40528 (to J. T.), HL26043 (to J. T. S.), and DK44322 (to A. P.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Present address: Chemistry Dept., University of North Carolina at Charlotte, Charlotte, NC 28223.

** To whom correspondence should be addressed: Bioscience Division, Mail Stop M-888, Los Alamos National Laboratory, Los Alamos, NM 87545. Tel.: 505-6672690; Fax: 505-6672891; E-mail: jtrewhella@lanl.gov.

Published, JBC Papers in Press, December 20, 2000, DOI 10.1074/jbc.C000857200


    ABBREVIATIONS

The abbreviations used are: CaM, calmodulin; cPKA, catalytic subunit of the cAMP-dependent protein kinase; MLCK, myosin light chain kinase; Delta NCaM, deletion mutant of calmodulin missing residues 2-8; skMLCK, skeletal muscle myosin light chain kinase; MOPS, 4-morpholinepropanesulfonic acid; AMPPNP, adenosine 5'-(beta ,gamma -imino)triphosphate.


    REFERENCES
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES


1. Means, A. R. (2000) Mol. Endocrinol. 14, 4-13[Free Full Text]
2. Hanks, S. K., and Quinn, A. M. (1991) Methods Enzymol. 200, 38-62[Medline] [Order article via Infotrieve]
3. Knighton, D. R., Zheng, J. H., Ten Eyck, L. F., Ashford, V. A., Xuong, N. H., Taylor, S. S., and Sowadski, J. M. (1991) Science 253, 407-414[Medline] [Order article via Infotrieve]
4. Krueger, J. K., Padre, R. C., and Stull, J. T. (1995) J. Biol. Chem. 270, 16848-16853[Abstract/Free Full Text]
5. Gallagher, P. J., Herring, B. P., Trafney, A., Sowadsi, J., and Stull, J. T. (1998) J. Biol. Chem. 268, 26578-26582[Abstract/Free Full Text]
6. Kemp, B. E., and Pearson, R. B. (1991) Biochim. Biophys. Acta 1094, 67-76[Medline] [Order article via Infotrieve]
7. Krueger, J. K., Olah, G. A., Rokop, S. E., Zhi, G., Stull, J. T., and Trewhella, J. (1997) Biochemistry 36, 6017-6023[CrossRef][Medline] [Order article via Infotrieve]
8. Krueger, J. K., Zhi, G., Stull, J. T., and Trewhella, J. (1998) Biochemistry 37, 13997-14004[CrossRef][Medline] [Order article via Infotrieve]
9. Babu, Y. S., Bugg, C. E., and Cook, W. J. (1988) J. Mol. Biol. 204, 191-204[Medline] [Order article via Infotrieve]
10. Persechini, A., and Kretsinger, R. H. (1988) J. Biol. Chem. 263, 12175-12178[Abstract/Free Full Text]
11. Meador, W. E., Means, A. R., and Quiocho, F. A. (1993) Science 262, 1718-1721[Medline] [Order article via Infotrieve]
12. Meador, W. E., Means, A. R., and Quiocho, F. A. (1992) Science 257, 1251-1255[Medline] [Order article via Infotrieve]
13. Heidorn, D. B., Seeger, P. A., Rokop, S. E., Blumenthal, D. K., Means, A. R., Crespi, H., and Trewhella, J. (1989) Biochemistry 28, 6757-6764[Medline] [Order article via Infotrieve]
14. Ikura, M., Clore, G. M., Gronenborn, A. M., Zhu, G., Klee, C. B., and Bax, A. (1992) Science 256, 632-638[Medline] [Order article via Infotrieve]
15. Osawa, M., Tokumitsu, H., Swindells, M. B., Kurihara, H., Orita, M., Shibanuma, T., Furuya, T., and Ikura, M. (1999) Nat. Struct. Biol. 6, 819-824[CrossRef][Medline] [Order article via Infotrieve]
16. Persechini, A., Gansz, K. J., and Paresi, R. J. (1996) J. Biol. Chem. 271, 19279-19282[Abstract/Free Full Text]
17. Gao, Z.-H., Zhim, G., Herring, B. P., Moomaw, C., Deogny, L., Slaughter, C. A., and Stull, J. T. (1995) J. Biol. Chem. 270, 10125-10135[Abstract/Free Full Text]
18. Heidorn, D. B., and Trewhella, J. (1988) Biochemistry 27, 909-915[Medline] [Order article via Infotrieve]
19. Gallagher, S. C., Callaghan, A., Zhao, J., Dalton, H., and Trewhella, J. (1999) Biochemistry 38, 6752-6760[CrossRef][Medline] [Order article via Infotrieve]
20. Svergun, D. I., Semenyuk, A. V., and Feigin, L. A. (1988) Acta Crystallogr. Sect. A 44, 244-250[CrossRef]
21. Zhao, J., Hoye, E., Boylan, S., Walsh, D. A., and Trewhella, J. (1998) J. Biol. Chem. 273, 30448-30459[Abstract/Free Full Text]
22. Peitsch, M. C. (1996) Biochem. Soc. Trans. 24, 274-279[Medline] [Order article via Infotrieve]
23. Sanchez, R., and Sali, A. (1997) Curr. Opin. Struct. Biol. 7, 206-214[CrossRef][Medline] [Order article via Infotrieve]
24. Krueger, J. K., Bishop, N. A., Blumenthal, D. K., Zhi, G., Becknigham, K., Stull, J. T., and Trewhella, J. (1998) Biochemistry 37, 17810-17817[CrossRef][Medline] [Order article via Infotrieve]


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