From the Department of Biomolecular Engineering,
Graduate School of Engineering, Tohoku University, Aoba-yama 07, Aoba-ku, Sendai 980-8579, Japan, the ¶ Hokkaido National
Industrial Research Institute, Tsukisamu-higashi, Sapporo 011-8511, Japan, the
Rational Drug Design Laboratory, Matsukawa, Fukushima
960-1242, Japan, and the ** Institute for Protein Research, Osaka
University, Yamadaoka, Suita 565-0871, Japan
Received for publication, January 18, 2001, and in revised form, April 4, 2001
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ABSTRACT |
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A structural and
thermodynamic study of the entropic contribution of salt bridge
formation to the interaction between hen egg white lysozyme (HEL) and
the variable domain fragment (Fv) of anti-HEL antibody, HyHEL-10, was
carried out. Three Fv mutants (HD32A, HD96A, and HD32AD96A) were
prepared, and the interactions between the mutant Fvs and HEL were
investigated. Crystallography revealed that the overall structures of
these mutant complexes were almost identical to that of wild-type Fv.
Little structural changes were observed in the HD32AD96A mutant-HEL
complex, and two water molecules were introduced into the mutation
site, indicating that the two water molecules structurally compensated
for the complete removal of the salt bridges. This result suggests that the entropic contribution of the salt bridge originates from
dehydration. In the singly mutated complexes, one water molecule was
also introduced into the mutated site, bridging the antigen-antibody
interface. However, a local structural difference was observed in the
HD32A Fv-HEL complex, and conformational changes occurred due to
changes in the relative orientation of the heavy chain to the light
chain upon complexation in HD96A Fv-HEL complexes. The reduced affinity of these single mutants for the antigen originates from the increase in
entropy loss, indicating that these structural changes also introduced
an increase in entropy loss. These results suggest that salt bridge
formation makes an entropic contribution to the protein
antigen-antibody interaction through reduction of entropy loss
due to dehydration and structural changes.
The ability of proteins to bind to one another in a highly
specific manner is an important feature of biological phenomena, and
the mechanism of antibody specificity and affinity toward target
proteins has been studied as a model of protein-protein interactions
(1). X-ray crystal structures of antibodies complexed with
proteinaceous antigens (2-6) suggest that the strict specificity of an
antibody originates from a high degree of complementarity between the
antigen-combining sites and the antigen. The complementarity is
composed of two factors: 1) apolar surfaces interacting by van der
Waals contact, where the surfaces of the antibody and antigen often fit
together to exclude most hydrated water molecules from the interface
and favorable close van der Waals contacts are formed; and 2) polar
surfaces interacting by hydrogen bond and salt bridge formation, where
the precise positioning of atoms allows charge-charge interactions
(i.e. salt bridge formation and hydrogen bond formation).
To elucidate the roles of the noncovalent forces involved in
antigen-antibody complementary association, both structural and thermodynamic information are crucial (7). X-ray crystal analysis can
clarify the structural aspects for the complementarity of the
interactions (2-5), and titration calorimetry can provide useful
information for the quantitative assessment of the contribution of
residues to the interaction (8-10). Thus, the combination of these two
approaches should give more precise insight into proteinaceous antigen-antibody interactions (11).
We have focused on the interaction between hen egg white lysozyme
(HEL)1 and its monoclonal
antibody HyHEL-10 (12-15), whose structural features have been
analyzed by x-ray crystallography (16) in the Fab-HEL complex. The
bacterial expression system of the HyHEL-10 Fv fragment, which consists
of the associated variable domains of an antibody, has been established
(17, 18), and the interactions have been thermodynamically investigated
using mutant Fv fragments (19-21). Recently, we reported the crystal
structure of the HyHEL-10 wild-type (WT) Fv-HEL complex at a resolution
of 2.3 Å (22). The combination of thermodynamic data with structural
results should be powerful for the precise description of the mutant
Fv-HEL interactions.
The role of salt bridge formation in antigen-antibody
interaction is particularly important since electrostatic
complementarity is critical for high specificity and affinity of
protein antigen-antibody interactions (2-5). The results of
alanine-scanning mutagenesis of protein-protein complexes have
demonstrated that, in the majority of cases, mutations of polar and
charged residues result in complex destabilization (23, 24). Two salt
bridges have been observed in the Fv-HEL complex; Lys97 of
HEL electrostatically interacts with both Asp32 and
Asp96 of HyHEL-10 VH (Fig.
1). We recently reported the role of salt bridge formation in the HyHEL-10 Fv-HEL complex from the thermodynamic viewpoint (21). We replaced Asp32 in VH with Ala, Asn, and
Glu and observed increases in both negative enthalpy and entropy
changes. We concluded that salt bridge formation makes an entropic
contribution to the interaction. Thus, structural analyses of mutant
Fv-HEL complexes should give further insights into the molecular basis
for the entropic contribution of the salt bridge to the
interaction.
INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
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Fig. 1.
Salt bridge in HyHEL-10 Fv-HEL
interface. Stereo view of the C stick diagram of wild-type
Fv-HEL complex. Green, VL; sky
blue, VH; pink, HEL. The residues participating
in salt bridge formation were drawn in orange. Figure was
generated with WebLab Viewer (MSI). Numbering of the antibody residues
follows that of Kabat et al. (38).
Here, using isothermal titration calorimetry and x-ray crystallography,
we report the effect of deleting antibody side chains participating in
salt bridge formation on the complex formation. Moreover, we discuss,
from the structural viewpoint, the molecular basis for the entropic
contribution of the salt bridge to the protein antigen-antibody interaction.
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MATERIALS AND METHODS |
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Site-directed Mutagenesis-- The general recombinant DNA technology was essentially that of Sambrook et al. (25). Site-directed mutagenesis was performed with phagemid pTZ18U (Bio-Rad, Tokyo, Japan) according to the method of Kunkel (26). The DNA oligonucleotides for mutation at sites 32 and 96 of VH were 5'-ATCACCTCGGCCTACTGGTCG-3' and 5'-GCGAACTGGGCCGGCGACTAC-3', respectively (mutated sites are underlined). Correctness of mutations was confirmed by DNA sequencing using an Auto-Read sequencing kit and an ALF express sequencer (Amersham Pharmacia Biotech, Tokyo, Japan).
Preparation of HyHEL-10 Mutant Fv-HEL Complex-- The mutant Fv fragment was prepared essentially as described previously (17). The purified Fv fragment was concentrated by ultrafiltration centrifugation (0.45-µm membrane) at 3000 × g (Centriprep-10). The antigen, HEL (Seikagaku-Kogyo Inc., Tokyo, Japan), was purified on SP-Sepharose FF (Amersham Pharmacia Biotech), followed by gel filtration on 75 pg of Superdex equilibrated with phosphate-buffered saline. Purities of the proteins were confirmed by SDS-polyacrylamide gel electrophoresis in the buffer system described by Laemmli (27). The eluate was lyophilized and dissolved in water at a concentration of 0.54 mM prior to use.
Circular Dichroism Spectroscopy of Mutant Fv Fragment-- Circular dichroism (CD) spectra in the near- and far-UV regions were obtained with a Jasco J-720 spectropolarimeter (Tokyo, Japan) equipped with a water bath to control the temperature at 25 °C. The concentration of antibody fragments was 0.1 mg/ml for the near- and far-UV experiments. Sixteen scans were averaged to obtain the spectrum. Cells with path lengths of 1 and 0.1 mm were used for near- and far-UV data acquisition, respectively.
Inhibition Assay of HEL Enzymatic Activity-- Experimental procedure for inhibition assay has been in principle followed by Ueda et al. (17). In brief, the various concentrations of the Fv fragment were mixed with 1.5 µM HEL and incubated at 25 °C for 1 h in 30 µl of phosphate-buffered saline. Each mixture was then added to 970 µl of 50 mM NaH2PO4 buffer (pH 6.20, adjusted with NaOH) containing 340 µg of Micrococcus luteus cells. The initial rate of the decrease in A540 nm was monitored at 25 °C.
Isothermal Titration Calorimetry-- Thermodynamic parameters of the interactions between HEL and the wild-type or mutant Fv fragments were determined by isothermal microtitration calorimetry using an Omega titration calorimeter (MicroCal, Inc.) (28). The experimental conditions were as follows. In a calorimeter cell, the Fv fragment at a concentration of 5 µM in 50 mM phosphate buffer (pH 7.2) containing 200 mM NaCl was titrated with a 125 µM solution of HEL in the same buffer at four different temperatures (25 °C, 30 °C, 35 °C, and 40 °C). The ligand solution was injected 16 times in portions of 7 µl over 15 s. Thermogram data were analyzed with the computer program Origin (MicroCal, Inc.).
The enthalpy change (H) and binding constant
(Ka) on antigen-antibody interaction were
directly obtained from the experimental titration curve. The Gibbs free
energy change (
G =
RT ln
Ka) and the entropy change (
S = (
G +
H)/T) on the
association were calculated from
H and
Ka. The heat capacity change
(
Cp) was estimated from the temperature
dependence of the enthalpy change.
Estimation of Protein Concentration--
The concentration of
HEL was estimated using A
Crystallization, Data Collection, and Structural Determination of the HyHEL-10 Mutant Fv-HEL Complex-- The HyHEL-10 mutant Fv fragment-HEL complexes were crystallized as described previously (22). The best crystals of the Fv-HEL complex were grown from 100 mM Hepes buffer (pH 7.6-7.8), 9-11% w/v polyethylene glycol 6000, and 7-9% w/v 2-methyl-2,4-pentanediol. They were elongated bipyramid-shaped crystals.
All crystallographic data were collected on a DIP-2000 diffractometer
(MAC Science, Yokohama, Japan) with a 200-mm diameter imaging plate
(CuK radiation; 50 kV, 90 mA). The diffraction images were
integrated with the hkl program DENZO,2 and the
intensity data were processed with SCALA,2 and AGROVATA
(32) in the CCP4 suite (33). The crystallographic data and the
statistics of the data collection are summarized in Table
I.
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The crystals of these three mutant Fv-HEL complexes
were isomorphous with that of wild-type complex. The structure of
HEL-Fv was determined by a molecular replacement method with the
program AMoRe (34) in the CCP4 package. The model coordinates of the mutant complexes were derived from the wild-type complex structure (Protein Data Bank code 1c08). Refinements of the structure of the
Fv-HEL complex were carried out with the programs XPLOR (35) and REFMAC
(36) as described previously (22). The atomic parameters of the mutant
Fv-HEL complexes have been deposited in the Protein Data Bank (codes
1IC4, 1IC5, and 1IC7 for HD32A, HD96A, and HD32AD96A, respectively).
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RESULTS |
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Secretory Expression, Preparation, and Spectral Properties of Mutant Fv Fragments
To elucidate the structural and thermodynamic effects of deleting salt bridges in the HyHEL-10 Fv-HEL complex, we constructed two new mutants (HD96A and HD32AD96A). These new mutants and the HD32A mutant were expressed in Escherichia coli, and the mutant Fv fragments were purified by affinity chromatography using HEL-Sepharose, followed by gel filtration on 75 pg of Superdex. Purities of greater than 95% were obtained with these steps, and the purified Fv fragments were subjected to precise analysis. The final yield of each mutant was greater than 10 mg/liter of culture.
To examine the effect of mutation on the conformational stability, we measured the peptide and aromatic CD spectra of the mutants under the native condition (pH 7.2, 25 °C). Comparison of these CD spectra with those of the WT revealed no differences in the far- and near-UV CD spectra of wild-type Fv and mutant Fv fragments (data not shown). The overall CD spectra of three mutants were almost identical to the CD spectrum of WT, indicating that the secondary and tertiary structures of the mutants are quite similar to the structure of WT. Thus, the mutations have no significant influence on the overall structure.
Inhibition of Enzymatic Activity of Hen Lysozyme by Mutants
It has been shown that the HyHEL-10 Fv fragment inhibits
the enzymatic activity of its antigen, HEL, in the presence of a slight
molar excess of the Fv fragment, resulting from the binding of the Fv
to the active site of HEL (17). Therefore, the inhibition of the
enzymatic activity of HEL by three mutants (HD32A, HD96A, and
HD32AD96A) was investigated (Fig. 2).
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In comparison with wild-type Fv, these clones showed decreased inhibition toward HEL to different degrees. The HD32AD96A mutant Fv exhibited the largest degree of decreased inhibition of HEL enzymatic activity, suggesting that deleting both side chains resulted in a significant reduction of affinity for HEL.
Thermodynamic Analysis of the Interactions between Hen Lysozyme and Mutant Fv Fragments
To quantitatively investigate the interactions between mutants and HEL from a thermodynamic viewpoint, we performed an isothermal titration calorimetry study of the association between mutant Fv fragments and lysozyme under the same conditions as described previously (19-21). Calorimetric titration was performed at four different temperatures. Thermodynamic parameters (30 °C, pH 7.2) calculated from the titration curves are presented in Table II, and temperature dependences of the enthalpy and entropy changes are shown in Figs. 3 and 4, respectively.
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On comparison of all mutant Fv fragments with the wild-type Fv, the
negative values of the enthalpy change for the interaction between each
mutant and HEL were increased by 22 kJ mol1 at 30 °C.
The binding constants were decreased slightly in two mutants (HD32A and
HD96A) and significantly in one mutant (HD32AD96A), resulting in a
smaller change in the Gibbs energy. These results indicate that the
increases in the negative enthalpy change for each mutant Fv fragment
were compensated for by the increase in negative enthalpy change,
leading to the decrease in affinity. From the temperature dependence of
the enthalpy change shown in Fig. 4, the heat capacity change was
estimated to be 1.8-2.1 kJ mol
1 K
1 at
30 °C for the mutant Fvs, which was 0.4-0.7 kJ mol
1
K
1 larger than the
Cp of
wild-type Fv.
Crystal Structures of Mutant Fv-HEL Complexes
To investigate the mutant Fv-HEL complexes from the structural viewpoint, we determined the crystal structures of the three mutant-HEL complexes. Crystallographic data are summarized in Table I. The maximum resolution of the x-ray data used in the refinements ranged from 2.1 to 2.5 Å, and the R factors of the refined structures were 0.165-0.184 (37). The overall structures of these mutant complexes are almost identical to that of wild-type Fv.
The mutant Fv-HEL complexes were superimposed onto the wild-type Fv-HEL
complex. The root-mean-square differences between the C atoms of the
mutant Fv structures and those of the wild-type Fv are summarized in
Table III. The results demonstrated that
the overall structures of the HyHEL-10 mutant Fv-HEL complexes are similar to that of the HyHEL-10 wild-type Fv-HEL complex (Table III).
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No major changes in the relative orientations of VL, VH, and HEL were
observed in the HD32A mutant Fv-HEL and HD32AD96A-HEL complexes.
However, superimposition of the C coordinates of Fv (VL and VH) of
the HyHEL-10 HD96A mutant complex onto those of the wild-type Fv
complex yielded root-mean-square differences of 0.21 for VL, 0.26 for
VH, and 0.58 for HEL. These root-mean-square differences originated
from the different relative orientations of Fv to HEL in the HD96A
mutant complex and the wild-type complex (Fig.
5).
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The calculated interfacial areas of the mutant Fv-HEL complexes were 1715, 1729, and 1722 Å2 for the HD32A, HD96A, and HD32AD96A mutant complexes, respectively (Table IV); these values were about 100 Å2 smaller than the value for the wild-type Fv (1835 Å2). The most significant decreases in the areas, >20 Å2, were in Asn19, Arg73, and Asn77 of HEL.
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Local Structural Changes Observed in Mutant Fv-HEL Complexes
HD32A Mutant-HEL Complex--
Although little structural change
occurred in the antigen HEL in the HD32A Fv-HEL complex, a structural
difference was observed in the complementarity-determining region 1 in
the heavy chain (CDR-H1) (Fig. 6). One
water molecule (W33) was introduced into the mutation site,
bridging Lys97 of HEL with the main chain of CDR-H1 (Fig.
6A). Tyr53 in CDR-H2 penetrated into the
antigen, making more van der Waals contacts with HEL (Fig.
6B). In addition, one water molecule (W2) formed
a hydrogen bond with the hydroxyl group of HTyr53, bridging
the loop of the lysozyme with the antibody. These observations suggest
that deletion of the carboxyl group at site 32 in VH led to the local
structural changes of the antibody in the complex.
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HD96A Mutant-HEL Complex--
No structural changes were observed
in the Fv of the HD96A Fv-HEL complex, except at the mutated site. One
water molecule was introduced into the mutation site (W15,
Fig. 7), bridging HAsp32 and
HAla96 with HAsn94, and Lys97 of
HEL, respectively. The structural change of the CDR-H3 and the change
of the relative orientation of Fv to HEL occurred, resulting from the
deposition of the water molecule. These results suggest that the effect
of deleting the Asp96 side chain in VH is compensated for
by introducing one water molecule and the change of relative
orientation of each chain.
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HD32AD96A Mutant-HEL Complex--
In contrast to the HD32A Fv-HEL
and HD96A Fv-HEL mutant complexes, there were few structural changes in
both the antigen and antibody of the HD32AD96A mutant Fv-HEL complex.
One water molecule (W4) was located in the mutated site and
formed two hydrogen bonds, filling a local structurally imperfect
complementarity (Fig. 8A). In
addition, one water molecule (W123) was retained in the
interface, participating in hydrogen-bonding networks in the
antigen-antibody interface (Fig. 8B).
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DISCUSSION |
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We recently reported the entropic contribution of salt bridge formation to the HyHEL-10 Fv-HEL interaction (21). To obtain the structural basis for the contribution to the interaction, two mutants (HD96A and HD32AD96A) were newly constructed, and the interactions of these two mutants and a third mutant (HD32A) with HEL were investigated by thermodynamic and structural analyses. As shown previously (15, 42) and in the present study, each mutation does not lead to major structural changes in the Fv fragment, and the stability of each mutant is almost identical to that of wild-type Fv. Thus, the structural and thermodynamic changes in the antigen-antibody complexes by mutation do not originate from changes in the structure of the mutants in the antigen-free state. In the following subsections, we discuss and correlate our thermodynamic and structural findings.
Thermodynamic Analysis of Mutant Fv-HEL Interactions--
The
values for both the enthalpic (H) and entropic
(
T
S) contributions to the interaction
between the mutants and HEL became increasingly negative in the order
HD32A < HD96A < HD32AD96A. The binding constants
(Ka) were decreased slightly in two mutants (HD32A
and HD96A) and significantly in one mutant (HD32A96A), resulting in a
smaller change in the Gibbs energy (
G). These results
indicate that deletion of salt bridges in the HyHEL-10 Fv-HEL complex
is enthalpically favorable and entropically unfavorable, suggesting
that the salt bridges in the HyHEL-10 Fv-HEL interaction make an
entropic contribution to the interaction. Substitution of
Asp32 with other residues (e.g. Asn and Glu)
also led to increases in both negative enthalpy and entropy changes
(21), which is almost identical to the results reported here. Thus, we
conclude that the salt bridges in the HyHEL-10-HEL complex make an
entropic contribution to the interaction.
Precise analyses of another protein antigen-antibody interaction, namely the HyHEL-5-HEL interaction, demonstrated that salt bridge formation made a significant contribution to the interaction (42-44). The salt bridge formed in an environment of low dielectric constant and, thus, the contribution of the salt link may be highly enthalpic (43, 44). On the other hand, an entropic contribution of the salt bridge was indicated in the HyHEL-10-HEL interaction.
To correlate the results obtained from thermodynamic analyses with structural information, the crystal structures of mutant Fv-HEL complexes were solved. We now discuss the role of salt bridge formation in the HyHEL-10-HEL interaction on the basis of structural information.
Comparison of the Mutant Fv-HEL Structure with the Wild-type Fv-HEL Structure-- Mutations did not affect the crystal packing. Thus, the local structural changes due to the mutations, including interface solvents, can be discussed directly by comparison of the mutant structures with the wild-type structure.
The overall structures of the mutant Fv-HEL complexes are similar to
that of the wild-type Fv-HEL complex (Fig. 5). However, the area of HEL
covered by mutant Fvs decreased by 46-84 Å2 in comparison
with the wild-type Fv-HEL complex, indicating a decrease in burial of
surface areas by mutation. The changes in thermodynamic parameters can
be accounted for in terms of changes in solvent-accessible polar and
apolar surface area (1, 45-49). Thus, the Gibbs energy changes of the
mutant Fv-HEL interactions have been estimated based on the polar and
apolar buried surface areas of the crystal structures. On comparison of
all mutant Fv fragments with the wild-type Fv, the calculated negative
values of the Gibbs energy change for the mutant-HEL interactions were decreased by 9.2, 8.6, and 10.0 kJ mol1 for HD32A, HD96A,
and HD32AD96A, respectively, at 30 °C. These values indicate that
the degree of reduction of the changes in polar and apolar surface
areas does not correspond to that of the decrease in affinity for the
antigen determined by experiments (Table II), suggesting the
compensation by other non-covalent bond formations.
The changes in the interaction between the double mutant (HD32AD96A)
and HEL reflect the complete deletion of salt bridges in the
HyHEL-10-HEL complex. Double mutation led to an order of magnitude
decrease in affinity (Table II), suggesting that recognition of the
amino group in Lys97 by Asp residues made a positive
contribution to the interaction. Little changes, including the relative
orientation of three chains and local loop structures, were observed in
the double-mutant complex. Two water molecules (Fig. 8, W4
and W123) were introduced into the mutated sites. These two
water molecules bridge the antigen-antibody interface by hydrogen
bonding (Fig. 8). These results indicate that structural compensation
by water molecules leads to the increase in negative values of
Cp and
H, suggesting that the
salt bridges make an entropic contribution through complete dehydration.
The negative values of heat capacity change
(Cp) calculated for each mutant complex
increased by 0.4-0.7 kJ K
1 mol
1 in
comparison with the wild-type value (Table II, Fig. 3). A negative
Cp has been proposed to be a consequence of
an increase in hydrophobic interaction (50, 51), burial of interfacial surface area (51), the presence of waters buried in the interface (52),
and/or local folding due to binding (53, 54). A recent report of the
interaction between mutant DNA gyrase and the antibiotic novobiocin
proposed that the negative values of
Cp
increase by ~200 ± 130 J K
1 mol
1
per interfacial water molecule (1 cal = 4.18 J) (52). The
increases in negative values of
Cp,
H, and
S may originate from refilling water
molecules in the interface.
On the other hand, in the HD32A and HD96A mutant complexes, at least one water molecule was newly retained at the mutated site, and structural changes (e.g. changes in the relative orientation of the variable domains and local loop structure) were observed.
The side chain at site 32 of VH is almost buried (the
calculated accessible surface area is 3 Å2) in the
complex. An Asp32 Ala mutation resulted in refilling
one water molecule at the mutated site in the mutant Fv-HEL complex
(Fig. 6). The water molecule makes two hydrogen bonds with the side
chain of HEL Lys97 and the main chain of the H chain
Ala32, bridging HEL with the Fv. In addition, the mutation
led to structural changes in CDR-H1, suggesting that structural changes
in the loop were introduced into CDR-H1 by substitution of
Asp32 with Ala. These results indicate that deleting the
side chain at site 32 of VH is compensated for by one water molecule
and local structural changes in the CDR-H1 loop. It has been suggested that the unfavorable entropy change results in part from reduction of
conformational flexibility in the antibody upon complexation (55,
56).
Replacement of Asp96 with Ala resulted in refilling one water molecule at the mutated site in the mutant Fv-HEL complex. The water molecule makes hydrogen bonds with Lys97 of HEL and HAsp32, HAsn94, and HAla96 of Fv, bridging HEL with the Fv. There were no significant changes in the main chains of the polypeptides (i.e. VH, VL, and HEL). The side chain at site 96 of VH is highly exposed (the calculated accessible surface area is 34 Å2). In addition, the relative orientation of VH to VL was changed by the mutation at site 96 of VH (Table III). These results suggest that one water molecule and structural changes (i.e. changes in the relative orientations of the chains) compensate for deleting the side chain at site 96 of VH. It has been indicated that the changes in the relative orientations of the chains reflected the induced fitting of the Fv to the protein antigen (57). The results reported here might suggest that removal of the side chain of Asp96 in VH HyHEL-10 Fv require the excess induced fitting for the recognition of the antigen.
It should be noted that the values of H of the
interaction between each of the single mutants (HD32A and HD96A) and
HEL are almost the same as that of the HD32AD96A mutant (Table II).
Moreover, the difference in affinity between each of the single mutants and the HD32AD96A mutant originates from the entropy change. Structural analyses have indicated that both a refilling water molecule and structural changes may lead to an increase in negative entropy change.
Therefore, an additional decrease in negative entropy change might be
required for further enhancement of the antibody affinity for the
antigen. Introducing two Asp residues in the antibody reduces the
entropy loss upon binding of the antibody to the antigen. These results
suggest that the salt bridge makes an entropic contribution to the
interaction through a decrease in entropy loss due to dehydration and
structural changes.
Role of Salt Bridge Formation in the HyHEL-10-HEL Interaction: Conclusion-- Salt bridge formation is a major contributor to various biomolecular interactions (17, 58). The salt bridges in the HyHEL-10-HEL complex, as shown here, are buried (Asp32-Lys97) and partially buried (Asp96-Lys97); deletion of these salt bridges led to structural compensation, including refilling water molecules. We conclude that salt bridge formation makes an entropic contribution to the interaction through a decrease in entropy loss due to dehydration and structural changes.
In this study, we found that deleting salt bridges resulted in an
unfavorable Gibbs energy change of 3-5 kJ mol1 per salt
bridge (Table III). Water molecules, however, were introduced into the
mutated sites, and the water molecules bridge the antigen-antibody interface by hydrogen bonding (Figs. 6-8). It has been suggested that
burial of water molecules makes a favorable contribution to
biomolecular interactions (59-62), and that one water molecule retained at the interface of proteins can make as many as four hydrogen
bonds, leading to a favorable contribution of ~10 kJ mol
1 per interfacial water molecule (51, 63). Supposing a
favorable contribution of the water newly retained in the interface,
salt bridges themselves in the interaction make a favorable
contribution of 13-15 kJ mol
1 to the Gibbs energy, which
is indistinguishable from the values for salt bridges in completely
dehydrated environments (e.g. the HyHEL-5-HEL interaction)
(43). Recent analyses of mutant human lysozymes have suggested the
contributions of salt bridge to the thermodynamic stability of the
protein correlated with the solvent inaccessibility of the salt
bridges, the salt bridge contribution was small when 100% accessible,
whereas it was about 9 kJ mol
1 if 100% inaccessible
(64). Computational studies of binding free energies based on the
results reported here may provide further precise description of the
interaction (23, 65-67).
Finally, the present study demonstrates that effects of mutation on the
interaction cannot be discussed without structural information (68,
69). Changes in the thermodynamic parameters include all changes due to
the mutation, including water refilling and/or conformational changes.
In this study, combination of thermodynamic data with the results of
structural analyses could give further insight into the role of salt
bridge formation in the interaction. Structural analyses of HyHEL-10
single mutant-HEL complexes, already investigated thermodynamically,
are now in progress; these analyses will be useful in correlating
thermodynamic parameters with structural changes due to mutation.
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ACKNOWLEDGEMENTS |
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We thank Dr. Katsumi Maenaka (National Institute of Genetics, Mishima, Japan) for critically reading the manuscript and Dr. Kazufumi Takano (Osaka University, Osaka, Japan) for valuable comments concerning the titration calorimetry.
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FOOTNOTES |
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* This work was supported in part by a fellowship for Japanese junior scientists (to M. S.) and a grant-in-aid for younger scientists (to K. T.) from the Japan Society for the Promotion of Science; by grants-in-aid for priority areas from the Ministry of Education, Science, Sports, and Culture of Japan; and by the Proposal-based R&D Promotion Program and the Industrial Technology Research Grant Program in 2000 from the New Energy and Industrial Technology Development Organization of Japan.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The atomic coordinates and the structure factors (code 1IC4, 1IC5, and 1IC7) have been deposited in the Protein Data Bank, Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, NJ (http://www.rcsb.org/).
§ To whom correspondence may be addressed. Fax: 81-22-217-7276; E-mail: tsumoto@mail.cc.tohoku.ac.jp.
To whom correspondence may be addressed. Fax:
81-22-217-6164; E-mail: kmiz@mail.cc.tohoku.ac.jp.
Published, JBC Papers in Press, April 10, 2001, DOI 10.1074/jbc.M100480200
2 Unpublished data.
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ABBREVIATIONS |
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The abbreviations used are:
HEL, hen egg white
lysozyme;
VH, variable region of immunoglobulin heavy chain;
VL, variable region of immunoglobulin light chain;
Fv, fragment of
immunoglobulin variable regions;
HD32A mutant, mutant HyHEL-10 Fv in
which Asp32 of the VH chain is replaced by Ala;
CDR, complementarity-determining region;
IPTG, isopropyl
-D-thiogalactopyranoside;
WT, wild-type.
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REFERENCES |
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