Fibroblast Growth Factor Homologous Factor 1B Binds to the C Terminus of the Tetrodotoxin-resistant Sodium Channel rNav1.9a (NaN)*

Chuan-ju LiuDagger §||, Sulayman D. Dib-HajjDagger §**, and Stephen G. WaxmanDagger §

From the Dagger  Department of Neurology and § Paralyzed Veterans of America/Eastern Paralyzed Veterans Association Neuroscience Research Center, Yale University School of Medicine, New Haven, Connecticut 06510 and the  Rehabilitation Research Center, Veterans Affairs Medical Center, West Haven, Connecticut 06516

Received for publication, February 20, 2001, and in revised form, March 15, 2001


    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

In this study we demonstrate a direct interaction between a cytosolic fibroblast growth factor family member and a sodium channel. A yeast two-hybrid screen for proteins that associate with the cytoplasmic domains of the tetrodotoxin-resistant sodium channel rNav1.9a (NaN) led to the identification of fibroblast growth factor homologous factor 1B (FHF1B), a member of the fibroblast growth factor family, as an interacting partner of rNav1.9a. FHF1B selectively interacts with the C-terminal region but not the other four intracellular segments of rNav1.9a. FHF1B binds directly to the C-terminal polypeptide of rNav1.9a both in vitro and in mammalian cell lines. The N-terminal 5-77 amino acid residues of FHF1B are essential for binding to rNav1.9a. FHF1B did not interact with C termini of two other sodium channels hNav1.7a (hNaNE) and rNav1.8a (SNS), which share 50% similarity to the C-terminal polypeptide of rNav1.9a. FHF1B is the first growth factor found to bind specifically to a sodium channel. Although the functional significance of this interaction is not clear, FHF1B may affect the rNav1.9a channel directly or by recruiting other proteins to the channel complex. Alternatively, it is possible that rNav1.9a may help deliver this factor to the cell membrane, where it exerts its function.


    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Ten distinct pore-forming alpha -subunits of sodium channels have been identified in the rat, and homologues have been cloned from various mammalian species including humans (1). Various alpha -subunits are expressed in a tissue- and developmentally specific manner (2). Aberrant expression patterns or mutations of voltage-gated sodium channel alpha -subunits underlie a number of human and animal disorders (3-7), including neuropathic pain (8, 9).

The alpha -subunits of sodium channels have been shown to interact with a number of proteins in addition to the auxiliary beta  subunits of the channel that affect their subcellular localization, their amplitude, and/or their kinetic properties. The C terminus of skeletal muscle and cardiac muscle alpha -subunits, SkM1 and SkM2, respectively, bind to syntrophin via their PDZ domain, thus linking these channels to the cortical actin network and extracellular matrix through the association of syntrophin with the dystrophin-associated protein complex (10). Brain alpha -subunits are phosphorylated at specific serine/threonine sites by protein kinase A that is anchored to the alpha -subunit via AKAP15 (11, 12). Receptor protein-tyrosine phosphatase (RPTPbeta ) interacts with brain alpha -subunits via its carbonic anhydrase homology extracellular domain and its intracellular phosphatase domain and phosphorylates tyrosine residues of the channel (13).

Peripheral sensory neurons in the dorsal root ganglia (DRG)1 and trigeminal ganglia produce pharmacologically and physiologically distinguishable Na+ currents, some of which are sensitive to the neurotoxin tetrodotoxin (TTX-S), and others which are resistant to this toxin (TTX-R). The TTX-R Nav1.9a (NaN) sodium channel is preferentially expressed in nociceptive neurons (14), and its expression is modulated in response to axotomy (14) and inflammation (15). The Nav1.9a channel produces a persistent TTX-R Na+ current that activates at potentials of -60 to -70 mV and is predicted to contribute to setting the resting membrane potential and modulating subthreshold electrogenesis in these neurons (16-18).

The yeast two-hybrid (Y2H) screening system (19) has proven to be an effective tool in identifying cytosolic partners of ion channels including voltage-gated K+ channels (20), AMPA receptors (21), the delta 2 glutamate receptor (22), and voltage-gated Na+ channels (23). To identify proteins that interact with the rNav1.9a C terminus, we screened brain Y2H cDNA library using a construct that encodes the C-terminal 198 residues of rNav1.9a as bait. These experiments identified fibroblast growth factor homologous factor 1B (FHF1B), which belongs to a subset of the FGF family (24), as a binding partner of rNav1.9a. It is possible that this interaction may help in exporting FHF1B to the exterior face of the cell membrane, or it may modulate the properties of rNav1.9a directly or indirectly by recruiting other factors to the channel complex.

    EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Antibodies and Mammalian Cell Lines-- The preparation and purification of an antiserum to rNav1.9 (NaN) has been previously described (25). Anti-GFP and anti-Myc polyclonal antibodies were purchased from CLONTECH. Human embryonic kidney (HEK) 293 cells and mouse NIH3T3 fibroblasts were used.

Plasmids and Clones-- Yeast expression vector pDBleu (Life Technologies) and pPC86 (Life Technologies) are fusion vectors for the linkage of proteins to the Gal4 DNA binding domain and to the VP16 transactivation domain, respectively. The segment encoding the C terminus (aa 1588-1765; GenBankTM accession number AF059030) of rNav1.9a (NaN) was amplified by PCR and cloned in-frame into the SalI/NotI sites of pDBleu (pDB-NaNC) to serve as bait in the screening assay. Fragments encoding sub-regions of the C terminus of rNav1.9a were also amplified and subcloned into the SalI/NotI sites of pDBleu: CR1+CR2 (aa 1588-1727; pDB-NaNCR1CR2), CR1 (aa 1588-1658; pDB-NaNCR1), CR2 (aa 1658-1727; pDB-NaNCR2), and UR (aa 1727-1765; pDB-NaNUR). Fragments encoding other cytoplasmic components of this channel were also cloned into the pDBleu vector: N terminus (aa 1-124; pDB-NaNN), L1 (aa 398-564; pDB-NaNL1), L2 (aa 802-1029; pDB-NaNL2), and L3 (aa 1288-1340; pDB-NaNL3). Similarly, cDNA fragments encoding the C termini of rNav1.8 (SNS) (aa 1725-1957; GenBankTM accession number X92184) and hNav1.7 (hNaNE) (aa 1753-1977; GenBankTM accession number X82835) sodium channel were cloned in-frame into the SalI/NotI sites of pDBleu vector to produce plasmids pDB-SNSC and pDB-hNEC, respectively. The full coding sequence of rat FHF1B was cloned in-frame into the SalI/NotI sites of pPC86 vector to generate plasmid pPC86-rFHF1B.

The bacterial expression vector pGEX-3X (Life Technologies) was used to produce recombinant proteins in Escherichia coli. The respective C-terminal fragments of rNav1.9 described above were subcloned in-frame into the BamHI/EcoRI sites of pGEX-3X to produce the plasmids pGEX-NaNC, pGEX-NaNCR1CR2, pGEX-NaNCR1, pGEX-NaNCR2, and pGEX-NaNUR.

Mammalian two-hybrid expression vectors pBIND and pACT (Promega) are fusion vectors for the linkage of proteins to the Gal4 DNA binding domain and to the VP16 transactivation domain, respectively. The complete coding sequence of rat FHF-1B cDNA was inserted in-frame into the SalI/NotI sites of pBIND fusion vector to produce plasmid pBIND-FHF1B. A cDNA fragment encoding the C terminus of rNav1.9a was inserted in-frame into the SalI/NotI sites of pACT fusion vector to produce plasmid pACT-NaNC. Plasmids for expression of tagged proteins in HEK293 cells were also constructed in the vectors pcDNA3.1/Myc-His (+) (Invitrogen) and pEGFP-N1 (CLONTECH). A cDNA fragment encoding the C terminus of rNav1.9a (described above) was inserted in-frame into the BamHI/EcoRI sites of pcDNA3.1/Myc-His(+)A to produce plasmid pcDNA3.1/Myc-His/NaNC. The complete coding sequence of FHF1B was subcloned in-frame into the BamHI/EcoRI sites of pEGFP-N1 vector (CLONTECH) to produce plasmid pFHF1B-GFP. This construct produces FHF1B-GFP fusion protein when introduced into mammalian cells.

All constructs were verified by sequencing their inserts. Sequencing was carried out at the Howard Hughes Medical Institute/Keck Biotechnology Resource Laboratory at Yale University. Sequence analysis was done using Lasergene (DNASTAR), GCG (GCG), and BLAST (National Library of Medicine) software.

RNA Preparation and Reverse Transcription-PCR-- Total cellular RNA was isolated from tissues of adult Harlan Sprague-Dawley rats by the single-step guanidinium isothiocyanate-acid phenol procedure (26). First-strand cDNA was synthesized from total RNA as previously described (27). PCR was carried out in two stages (27) for a total of 27 cycles by using a PTC 200 programmable thermal cycler (MJ Research).

FHF1B and GAPDH primers were used in a multiplex PCR reaction to determine the tissue distribution of FHF1B in adult rat tissues. FHF1B forward (5'-CACTCTAAAGGGACCGAAATGGAG-3') and reverse (5'-GAAGAGTTCTCAGCCAGGCTATG-3') primers amplify nucleotides 39-620 (GenBankTM accession number AF348446), whereas GAPDH forward (5'-TCACCACCATGGAGAAGGCTG-3') and reverse (3'-CCCTGTTGCTGTAGCCATATTC-3') primer amplify nucleotides 328-994 (GenBankTM accession number M17701). FHF1B and GAPDH primers were used at final concentrations of 2 and 0.2 µM, respectively, to reduce the inhibition of FHF1B amplification by excess GAPDH templates. Control PCR with water or RNA template produced no amplification products (data not shown).

Yeast Two-hybrid (Y2H) Library Screen-- Plasmid pDBNaNC (see above) was used as bait to screen rat brain pPC86 cDNA library (Life Technologies) using the protocol provided by the manufacturer with few modifications. Briefly, bait plasmid was first introduced into yeast MAV203 strain that contains three reporter genes, HIS+, URA+, and Lac Z (Life Technologies), and transformants were selected on defined medium lacking leucine. The rat brain cDNA library in pPC86 was then transformed into the resultant Leu+ yeast strain and plated on medium lacking tryptophan, leucine, histidine, and uracil but containing 25 mM 3-amino-1,2,4-trizone (SD leu-/trp-/his-/ura-/3AT+). After incubation for 7 days at 30 °C, colonies were screened for beta -galactosidase by a filter lift assay (28). Individual pPC86 recombinant plasmids were further verified for interaction with bait by repeating the Y2H assay.

Assay of Protein-Protein Interactions by Y2H Assay-- Three independent colonies were analyzed for the interaction in yeast between two proteins, one of which was fused to the Gal4 DNA binding domain and the other to the VP16 transactivation domain. The procedures of Vojtek et al. (29) and Hollenberg et al. (28) were followed for (i) growing and transforming the yeast strain MAV203 with the selected plasmids and (ii) testing by the beta -galactosidase assay and by growth phenotypes on (SD leu-/trp-/his-/ura-/3AT+) plates and on plates containing 5-fluoroorotic acid (SD leu-/trp-/5FOA+).

Expression and Purification of Glutathione S-Transferase (GST) Fusion Proteins-- For expressing GST fusion proteins, the appropriate plasmids (pGEX-NaNN (N terminus, aa 1-124); pGEX-NaNL1 (L1, aa 398-564); pGEX-NaNL2 (L2, aa 802-1029); pGEX-NaNC (C terminus, aa 1588-1765), pGEX-NaNCR1CR2, pGEX-NaNCR1, pGEX-NaNCR2, and pGEX-NaNUR (see above for coordinates)) were transformed into E. coli DH5alpha (Life Technologies). The fusion proteins were affinity-purified on glutathione-agarose beads as previously described (30).

In Vitro Binding Assay-- To examine the binding of FHF1B and its derivatives to the C terminus of rNav1.9 in vitro, glutathione-Sepharose beads (50 µl) preincubated with purified GST (0.5 µg), serving as control, or GST-NaNC (0.5 µg) were incubated with extract (500 µg of protein) of HEK293 cells transfected with an expression plasmid encoding either full-length FHF1B-GFP or its derivatives (i.e. FHF1B-(5-181)-GFP, FHF1B-(42-181)-GFP, FHF1B-(77-181)-GFP, and FHF1B-(143-181)-GFP), as indicated, in 150 µl of buffer AM (10 mM Tris, pH. 7.9, 10% glycerol, 1 mM MgCl2) (31) supplemented with 100 mM KCl and 0.5 mg/ml bovine serum albumin (30). The bound proteins were denatured in sample buffer and separated by 12% SDS-PAGE, and GFP fusion proteins were detected by immunoblotting with anti-GFP antibodies (CLONTECH). Similarly, GST fusion proteins of C terminus derivatives (0.5 µg) were analyzed in this binding assay to further delineate the sequence in the C terminus of rNav1.9 that is responsible for the interaction with FHF1B.

Far Western Assay-- Far Western (overlay blot) assay was carried out according to Lee et al. (32) with minor modification. After a 1-h incubation with anti-GFP antibodies, extracts of HEK293 cells transfected with an expression plasmid encoding either GFP or FHF1B-GFP fusion protein were incubated with protein A-agarose at 4 °C overnight. After washing five times in cell lysis buffer (phosphate-buffered saline, pH 7.5, 1% Triton X-100, 5 mM EDTA), the purified GFP (control), and FHF1B-GFP were loaded onto 15% SDS-PAGE and subjected to electrophoresis. The proteins were electrotransferred to a nitrocellulose membrane at 85 mA for 2 h. The proteins on the blot were denatured and renatured by sequential washings in 0.1 M CZ solution (20 mM HEPES, pH 7.9, 20% glycerol, 0.1 M KCl, 5 mM MgCl2, 01.mM ZnCl2, 0.1 mM EDTA, 2 mM dithiothreitol) plus 0.02% polyvinylpyrrolidone and 6 M guanidine-HCl for 30 min, one time, then three times in 0.1 M CZ solution plus 0.02% polyvinylpyrrolidone, each for 2 h. After blocking with 5% bovine serum albumin, the membranes were incubated with 50 µg of purified GST-NaNC protein, followed by incubation with anti-NaN antibodies (0.2 µg/ml) for 1 h. The signal was detected using the ECL chemiluminescent system (Amersham Pharmacia Biotech).

Two-hybrid Assay for Interacting Proteins in Mammalian Cells-- Components of the mammalian two-hybrid assay (Promega) were used to study the interaction of FHF1B and the C terminus of rNav1.9 in NIH3T3 cells. NIH3T3 cells were grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum and 100 unit/ml antibiotics. When cultures reached about 50% confluence, the cells were transfected with 1 µg each of the indicated plasmids using Lipofectin (Life Technologies) according to the manufacturer's recommendations. Plasmid pUC19 was used as a carrier to bring the total amount of DNA in the transfection solution to 3 µg. The cultures were harvested 48 h after transfection and lysed, and firefly luciferase activity was determined according to manufacturer's recommendations.

Co-immunoprecipitation-- HEK293 cells were grown in 100-mm dishes in Dulbecco's modified Eagle's medium containing heat-inactivated 10% fetal bovine serum. After reaching 50% confluence, the cultures were co-transfected with the mammalian expression plasmids pFHF1B-GFP and pcDNA3.1Myc-His/NaNC. The cultures were harvested 48 h later, and cell extracts were prepared according to Liu et al. (33). After a 1-h incubation with either anti-Myc (25 µg/ml) (CLONTECH) or anti-NaN (20 µg/ml) antibodies, aliquots (200 µl) from this mixture were incubated with 30 µl of protein A-agarose (Life Technologies) at 4 °C overnight. After washing five times with immunoprecipitation buffer (34), the bound proteins were released by boiling in 20 µl of 2× SDS loading buffer for 3 min (30). The released proteins were examined by Western blotting with anti-GFP antibodies (CLONTECH), and the signal was detected using the ECL chemiluminescent system (Amersham Pharmacia Biotech).

    RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Identification of FHF1B as a Binding Partner of rNav1.9a-- The tetrodotoxin-resistant (TTX-R) sodium channel rNav1.9a (NaN) is expressed preferentially in peripheral sensory neurons and is down-regulated after axotomy (14). This channel is also expressed at low levels in central nervous system tissues (14, 35). A screen based on the yeast two-hybrid (Y2H) system (19) was performed to identify proteins that interact with rNav1.9a. For this purpose, we linked the five intracellular regions of rNav1.9a, namely, the N terminus, loop 1, loop 2, loop 3, and the C terminus to the Gal4 DNA binding domain (GAL4DBD) in the plasmid pDBleu. We used the respective constructs as baits to screen a library of rat brain cDNA expressed as fusion proteins to the VP16 acidic activation domain (VP16AD) in the vector pPC86.

A Y2H rat brain cDNA library (Life Technologies) was screened with the construct encoding the cytosolic C terminus of rNav1.9a (aa 1588-1765). We screened about 5 million clones and identified 23 clones that activated the three reporter genes. Further tests involved the re-transformation of yeast with the purified target plasmids and bait. Only 2 of the original 23 yeast clones expressed full-length proteins that interact with the C-terminal bait (not shown). The plasmids in these clones contained 2.96-kilobase pair identical inserts.

Sequence Analysis of Full-length rFHF1B-- The sequence of the two inserts matched that of the chicken fibroblast growth factor homologous factor gFHF1B (36) and represents the rat homologue of this factor (Fig. 1). This factor belongs to the FGF family of growth factors (24). An open reading frame of 546 base pairs encodes a polypeptide of 181 amino acids with a predicted mass of 20 kDa (Fig. 1A). The rat factor, rFHF1B, is highly conserved compared with the chicken counterpart with 87% identity at the nucleotide level (not shown) and 97% identity at the amino acid level (Fig. 1B). FHF1B lacks a canonical signal peptide, like the other members of this subfamily (24). Unlike FHF1A, which possesses a nuclear targeting signal as a result of alternative splicing, rFHF1B lacks such a signal (Fig. 1B).


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Fig. 1.   Sequence of rat FHF1B. A, deduced amino acid sequence of rFHF1B. The sequence of rFHF1B was deposited into GenBankTM (accession number AF348446). The FGF family signature motif is in boldface type. Recognition sites of multiple protein kinases were identified in this sequence: protein kinase A site (RKSS, aa 162-165); PKC sites (SGR, aa 107-109; SRK, aa 161-163); casein kinase 2 (TKDE, aa 34-37; SVFE, aa 88-91; SLHE, aa 150-153). B, amino acid sequence alignment of rFHF1B and other members of the FHF1 subfamily. Stars represent residues that are identical to those of rFHF1B. The bipartite components of the nuclear localization signal in FHF1A are underlined. h, human; m, mouse; g, chicken; and r, rat.

Multiplex PCR of FHF1B and GAPDH was used to investigate tissue distribution of FHF1B in adult rat tissue. FHF1B is readily detectable in central and peripheral nervous tissues including cerebrum, cerebellum, spinal cord, DRG, and trigeminal ganglia (Fig. 2). The rFHF1B fragment that is amplified from DRG tissues using these rFHF1B-specific primers shows identical sequence to that obtained from the rat brain cDNA library. This factor is expressed, however, at lower levels in other neural tissues such as superior cervical ganglia and retina and in the cardiac and skeletal muscles, whereas it is undetectable under these amplification conditions in liver, and kidney (Fig. 2). The tissue distribution of rFHF1B shows an overlap with the expression of rNav1.9a in DRG and trigeminal ganglia (14).


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Fig. 2.   Tissue distribution of rFHF1B by reverse transcription-PCR. Amplification products from DRG, trigeminal ganglia, cerebral hemisphere, cerebellum, and spinal cord (lanes 1-5, respectively) are consistent with a predicted size of 581 base pairs (bp) for rFHF1B and 652 base pairs for GAPDH. A faint rFHF1B signal is detectable in superior cervical ganglia, retina, cardiac muscle, and skeletal muscle (lanes 6-9). No detectable rFHF1B signal is seen in liver (lane 10) and kidney (lane 11). All samples show a GAPDH amplification product, confirming that the absence of rFHF1B signal is due to lack of gene expression rather than the quality of the cDNA template. M lanes contain the 100-base pair ladder marker (Amersham Pharmacia Biotech).

The Y2H assay was repeated to verify the interaction between rFHF1B and the C terminus of rNav1.9a. For this purpose, the plasmid encoding the C terminus of rNav1.9a linked to the Gal4DBD and the plasmid encoding rFHF1B fused to the VP16AD were used to co-transform the yeast strain MAV203. Plasmid pairs encoding c-Jun/c-Fos and Rb/lamin were used as positive and negative protein-protein interaction controls, respectively (Fig. 3A). The interaction between the C terminus polypeptide of rNav1.9a and rFHF1B was verified by beta  galactosidase assay and growth phenotype on selective media (Fig. 3A). Like the c-Jun/c-Fos pair, which is known to interact, our assays indicate that rFHF1B interacts with the C terminus of rNav1.9a in yeast, based on the activation of the LacZ reporter gene (Fig. 3A, left panel), growth on the plates lacking histidine and uracil but containing 3-amino-1,2,4-trizole (Fig. 3A, middle panel), and inhibition of growth on plates containing 0.2% 5-fluoroorotic acid (Fig. 3A, right panel).


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Fig. 3.   Binding of rFHF1B to the C-terminal polypeptide of rNav1.9a. A, yeast two-hybrid assay to test the interaction between proteins fused to the VP16 AD and proteins fused to the Gal4 DBD. Each pair of plasmids, as indicated, encoding proteins fused to VP16 (above the line) in the vector pPC86 (i.e. pPC86-c-jun, pPC86-FHF1B, and pPC86-Rb) and those encoding proteins fused to Gal4 (under the line) in the vector pDBleu (i.e. pDB-c-fos, pDB-NaNC, and pDB-lamin) were co-transfected into yeast strain MAV203. Yeast transformants were selected on SD leu-/trp- plates and tested for beta -galactosidase activity (left panel) for growth on plates lacking histidine, uracil, and containing 3AT (middle panel, SD leu-/trp-/his-/ura-/3AT+) and for growth on plates containing 5 fluoroorotic acid (5FOA) (right panel, SD leu-/trp-/5FOA+). The known interaction between c-Jun and c-Fos is used as a positive control, whereas the lack of interaction of Rb and lamin serves as a negative control. B, schematic diagram of the rNav1.9a. N terminus (N-term, aa 1-124), loop 1 (L1, aa 398-564), loop 2 (L2, aa 802-1029), loop 3 (L3, aa 1288-1340), and the C terminus (C-term, aa 1588-1765) represent the five cytoplasmic regions of the sodium channel. The four domains comprising the alpha -subunit are designated I-IV. C, comparison of the binding of rFHF1B to the individual intracellular segments of rNav1.9a. A plasmid encoding the full coding sequence of rFHF1B fused to the VP16AD in the vector pPC86 and a plasmid encoding one of the five cytoplasmic region of rNav1.9a fused to the Gal4DBD in the vector pDBleu were co-transfected into the MAV203 yeast strain. Yeast transformants were selected on SD leu-/trp- plates. Three independent yeast transformants for each pair of plasmids were transferred onto a nitrocellulose membrane, and the beta -galactosidase activity was determined as previously described (28, 29).

The other cytoplasmic polypeptides of rNav1.9a (Fig. 3B) were tested for their ability to interact with rFHF1B. Filter-based beta -galactosidase assays were used to determine if the co-expression of the various rNav1.9a/Gal4DBD and rFHF1B/VP16AD fusion proteins activated the reporter LacZ gene. As shown in Fig. 3C, rFHF1B selectively interacts with the C-terminal polypeptide of rNav1.9a, among the five intracellular polypeptides tested in this assay.

rFHF-1B Binds Directly to the C-terminal Polypeptide of rNav1.9a in Vitro-- To test whether rFHF1B binds to the C-terminal polypeptide of rNav1.9a in vitro, we expressed rFHF1B-GFP fusion protein in HEK293 cells and expressed the C-terminal polypeptide of rNav1.9a as a GST fusion protein (GST-NaNC). Affinity-purified GST and GST-NaNC immobilized on glutathione-Sepharose beads were incubated with cell lysates expressing either GFP or FHF1B-GFP. Purified GST or GST-NaNC did not pull down GFP protein in this assay (Fig. 4A, lanes 1-3). Purified GST did not pull down rFHF1B-GFP fusion protein (Fig. 4A, lane 5), whereas GST-NaNC efficiently pulled down the rFHF1B-GFP fusion protein (Fig. 4A, lane 6).


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Fig. 4.   Direct interaction between rFHF1B and the C terminus polypeptide of rNav1.9a in vitro. A, binding of rFHF1B to NaNC in vitro (GST pull-down assay). Purified GST (lanes 2 and 5) or GST-NaNC fusion protein (lanes 3 and 6) immobilized on glutathione-Sepharose beads were incubated with extracts prepared from HEK293 cells transfected with a plasmid encoding either GFP alone (lanes 1-3) or rFHF1B-GFP fusion protein (lanes 4-6). HEK293 proteins that are trapped by the interaction with GST or GST-NaNC (lanes 1 and 4, respectively) were examined by immunoblotting with anti-GFP antibodies. GFP and rFHF1B-GFP as well as an unknown protein are indicated. B, direct interaction of rFHF1B with NaNC as determined by Far Western blotting. GFP (lane 1) or rFHF1B-GFP (lane 2) proteins, immunoprecipitated by anti-GFP antibodies from HEK293 cells transfected by the corresponding plasmid, were separated by 12% SDS-PAGE, electroblotted onto nitrocellulose membranes. The membranes were incubated with 50 µg of purified GST-NaNC followed by immunodetection using anti-NaN antibodies.

We used an overlay binding assay to show that rFHF1B binds directly to the C-terminal polypeptide of rNav1.9a. GFP or rFHF1B-GFP proteins were immunoprecipitated with anti-GFP antibodies from lysates of HEK293 cells transfected with the corresponding expression plasmid. These immunoprecipitated proteins were separated by SDS-PAGE electrophoresis, electrotransferred and immobilized onto nitrocellulose membrane, and incubated with affinity-purified GST or GST-NaNC. Binding was then detected using anti-NaN antibodies. Fig. 4B shows that the purified GST-NaNC fusion protein binds to the purified rFHF1B-GFP (lane 2) but not to GFP alone (lane 1); GST did not bind to either rFHF1B-GFP or GFP (not shown).

FHF-1B Binds to the C-terminal Polypeptide of rNav1.9a in Vivo-- To test whether rFHF1B binds to the C-terminal polypeptide of rNav1.9a in vivo, we performed a mammalian two-hybrid assay in NIH3T3 cells containing a reporter plasmid in which the expression of the firefly luciferase gene was driven by five Gal4-specific enhancer elements (Fig. 5A). By analogy to the Y2H system, the interaction between the C-terminal polypeptide of rNav1.9a and rFHF1B brings together the Gal4DBD and the VP16AD of the fusion proteins and activates the reporter gene in NIH3T3 cells (Fig. 5A). Fig. 5B shows that these two proteins interact in mammalian cells as well as in yeast. Therefore, any post-translational modifications that may occur in mammalian cells, but that are absent in yeast, do not interfere with the interaction of rFHF1B and the C-terminal polypeptide of rNav1.9a.


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Fig. 5.   Interaction between rFHF1B and C terminus polypeptide of rNav1.9a in vivo. A, schematic representation of the two-hybrid assay in mammalian cells. The expression of the firefly luciferase is driven by five copies of the Gal4 DNA recognition sequence in the reporter plasmid (pG5luc). A fragment encoding the full open reading frame of rFHF1B is linked to a sequence encoding Gal4DBD in the vector pBIND-FHF1B. A fragment encoding the C terminus of rNav1.9a is linked to a sequence encoding the VP16AD in the vector pACT-NaNC. B, the plasmids, as indicated, were transfected into NIH3T3 cells. After incubation, the firefly luciferase activity was determined in cell lysates. C, co-immunoprecipitation (Ip) assay of fusion proteins expressed in HEK293 cells. Extracts prepared from HEK293 cells co-transfected with two expression plasmids encoding rFHF1B-GFP and Myc-tagged NaNC hybrid proteins were incubated with either polyclonal Myc antibodies (lane 2) or NaN antibodies (lane 3). The immunoprecipitated protein complex and cell extract (lane 1, which provides a positive control) were examined by immunoblotting (Wb) with an anti-GFP antibody. Bands for FHF1B-GFP, IgG, and protein X are indicated.

The result of the mammalian two-hybrid assay was confirmed by a co-immunoprecipitation assay (Fig. 5C). In this assay, the extracts of cells expressing both rFHF1B-GFP fusion protein (lane 1) and Myc-tagged C-terminal polypeptide of rNav1.9a were first incubated with either anti-Myc or anti-NaN antibodies, and the immunoprecipitated complexes were counter-tested with anti-GFP antibodies. A clear rFHF1B-specific band was present in the immunoprecipitated complexes brought down by either anti-Myc (lane 2) or anti-NaN (lane 3) antibodies, demonstrating that rFHF1B binds to the C terminus polypeptide of rNav1.9a in vivo.

The N-terminal Segment (aa 5-77) of rFHF1B Is Required for Association with the C-terminal Polypeptide of rNav1.9a-- A series of N-terminal deletions of rFHF1B were linked to GFP (Fig. 6A). Pull-down assays involving the immobilized GST (Fig. 6B, lanes 2, 5, 8, 11, and 14) and GST-NaNC (Fig. 6B, lanes 3, 6, 9, 12, and 15) and cell extracts prepared from HEK293 cells transfected with rFHF1B mutants fused to GFP (Fig. 6B, lanes 1, 4, 7, 10, 13) were performed to determine the ability of the various rFHF1B mutants to associate with the C-terminal polypeptide of rNav1.9a. Immunoblotting using anti-GFP antibodies shows that rFHF1B lacking the N-terminal pentapeptide binds GST-NaNC as efficiently as the full-length factor (compare lanes 3 and 6). Deletion of the N-terminal 42 residues significantly reduced the interaction of the two polypeptides (lane 9), whereas removal of 77 residues totally disrupted this interaction (lane 12). Not surprisingly, rFHF1B lacking the N-terminal 143 residues does not show any interaction with GST-NaNC (lane 15). As a negative control, GST did not pull down either of the rFHF1B derivative polypeptides (Fig. 6B, lanes 2, 5, 8, 11, 14). These data show that the N-terminal segment (aa 5-77) contains the molecular determinants for the interaction of rFHF1B with the C-terminal polypeptide of rNav1.9a.


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Fig. 6.   Identification of rFHF1B segments binding to rNav1.9a. Schematic diagram of FHF1B-GFP constructs used to map the binding of FHF1B to rNav1.9a. Numbers refer to the amino acid residues in FHF1B. Each segment (i.e. aa 1-4, 5-41, 42-76, 77-142, and 143-181) is encoded by a single exon. The binding or lack of binding of the various FHF1B segments to rNav1.9a, as revealed in B, are indicated by plus or minus signs, respectively. B, 0.5 µg of GST (lanes 2, 5, 8, 11, and 14) or 0.5 µg of GST-NaNC (lanes 3, 6, 9, 12, and 15) immobilized on glutathione-Sepharose beads was incubated with cell extracts prepared from HEK293 cells transfected with an expression plasmid encoding either full-length FHF1B-(1-181)-GFP (lanes 1-3), FHF1B-(5-181)-GFP (lanes 4-6), FHF1B-(42-181)-GFP (lanes 7-9), FHF1B-(77-181)-GFP (lane 10-12), or FHF1B-(143-181)-GFP (lane 13-15). The bound proteins and cell extracts (lanes 1, 4, 7, 10, and 13) were examined by immunoblotting with anti-GFP antibodies. For additional details, see "Experimental Procedures."

Conserved Segments of the C-terminal Polypeptide of rNav1.9a Are Important for rFHF1B Binding-- Alignment of the C-terminal polypeptides of all mammalian alpha -subunits (not shown) allowed us to divide this part of rNav1.9a polypeptide into two conserved regions (CR1 and CR2) of about 70 amino acids each and a terminal unique region (UR) of 38 amino acids. We subcloned fragments encoding the individual conserved and unique regions to identify the molecular determinants of rFHF1B binding. We first carried out Y2H assays using CR1, CR2, CR1+CR2, and UR GAL4DBD baits and full-length rFHF1B fused to VP16AD. These experiments demonstrate that conserved region 1 (CR1, aa 1588-1657) alone or together with conserved region 2 (CR1+CR2, aa 1588-1726) of the C-terminal polypeptide interact with rFHF1B, whereas the unique region (UR, aa 1727-1765) did not interact with this factor (data not shown). We also used a GST pull-down assay to determine quantitatively the efficiency of the interaction of the various constructs with rFHF1B (Fig. 7A). In these assays, comparable amounts of the various purified GST fusion proteins (Fig. 7C) were preincubated with glutathione-Sepharose beads (50 µl) followed by incubation with cell extracts from HEK293 cells expressing rFHF1B-GFP fusion protein (Fig. 7B, lane 1). The results confirm that the UR was neither capable of binding to this factor (Fig. 7B, lane 7) nor did its removal affect the interaction between rFHF1B and the conserved regions of the polypeptide (Fig. 7B, lane 4). Like the unique region, conserved region 2 alone did not interact with rFHF1B (Fig. 7B, lane 6). The removal of CR2, however, significantly reduced the strength of the interaction of CR1 with rFHF1B (compare lanes 4 and 5 of Fig. 7B). These results suggest that the binding site of rFHF1B straddles the CR1-CR2 junction or that CR2 influences the folding of CR1 such that the molecular determinants of rFHF1B binding are optimally accessible when CR2 is present. Further experiments are needed to distinguish between these two possibilities.


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Fig. 7.   rFHF1B binds to the conserved region in the C terminus of rNav1.9a. A, schematic diagrams of GST-NaNC fusion proteins used to map the sites of rFHF1B binding on the C-terminal polypeptide of rNav1.9a. The numbers refer to amino acid residues in the C terminus of rNav1.9a. CR1, CR2, and UR stand for conserved region 1, conserved region 2, and unique region, respectively. The strengths of the binding of rFHF1B to the various GST-NaNC segments as revealed in B are indicated. B, binding of rFHF1B by the C terminus of rNav1.9a and its derivatives. Glutathione-Sepharose beads carrying GST and GST-NaNC or its derivatives as indicated were incubated with extracts prepared from HEK293 cells transfected with an expression plasmid encoding rFHF1B-GFP fusion protein, and the bound FHF1B-GFP was detected by immunoblotting with anti-GFP antibodies. The FHF1B-GFP band is indicated. C, expression of free GST or GST linked to the C-terminal polypeptide of rNav1.9a or its derivatives. Samples of affinity-purified GST and GST-NaNC or its derivatives (0.5 µg) as indicated were examined by SDS-PAGE and Coomassie Blue staining. The positions of size markers in kDa are indicated.

rFHF1B Binds Specifically to rNav1.9a-- The length of the C-terminal polypeptides of the Na+ channel alpha -subunits from rat tissues varies from 177 for rNav1.9a to 251 for rNav1.3a (brain type III). Alignment of these polypeptides (not shown) shows that the similarity of the C-terminal polypeptide of rNav1.9a ranges from 50% for rNav1.1a (brain type I) to 56.7% for rNav1.5a (rSkM2). This similarity is 51.1 and 53.4% to rNav1.8a (SNS) and hNav1.7a (hNaNE), respectively, both of which, like Nav1.9a, are expressed preferentially in DRG and trigeminal neurons (37, 38). Therefore, we tested whether the C-terminal polypeptides of these two channels are capable of binding the rFHF1B protein in Y2H assays. The data in Fig. 8 shows that rFHF1B selectively binds to the C-terminal polypeptide of rNav1.9a but not to those of rNav1.8a or hNav1.7a.


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Fig. 8.   rFHF1B selectively associates with the C-terminal of rNav1.9a. The beta -galactosidase assay was used to test the interaction between rFHF1B and the C termini of the sodium channels rNav1.9a (NaNC), hNav1.7a (hNEC), and rNav1.8a (SNSC). The known interaction between c-Jun and c-fos serves as a positive control, whereas the lack of interaction of Rb and lamin serves as a negative control. Three independent yeast transformants for each pair of plasmids were transferred onto the nitrocellulose membrane, and the beta -galactosidase activity was determined as previously described (28, 29).


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

We used the yeast two-hybrid (Y2H) screen to identify protein partners of the TTX-R sodium channel rNav1.9a (NaN). We present evidence that rFHF1B, which is a member of the FGF family of growth factors, binds directly to the C-terminal polypeptide of rNav1.9a. This interaction is specific to rNav1.9a and is not seen in other Na+ channels that, like rNav1.9a, are preferentially expressed in DRG and trigeminal neurons. We also present evidence that localizes the binding site to specific segments of rNav1.9a C terminus polypeptide and rFHF1B, respectively. Our results provide the first evidence for a direct link between a growth factor and a voltage-gated sodium channel.

Sodium channels interact with multiple protein partners, affecting their localization and the amplitude and kinetics of the currents that they produce. The beta 2 subunit is proposed to increase the delivery of pore-forming alpha -subunits to the cell membrane (39), whereas the beta 1 subunit modulates the amplitude and rate of inactivation of recombinant alpha -subunits expressed in Xenopus oocytes (40, 41) and permits interaction of the sodium channel complex with the extracellular matrix proteins tenascin-C and -R (42-45). Skeletal and cardiac muscle sodium channels interact directly with the cytoskeletal element syntrophin, which may determine their localization (10). The interaction of brain sodium channels with AKAP15 is required for dopaminergic modulation (11). More recently, the C-terminal polypeptide of rNav1.2 (brain type II) was shown to bind calmodulin, suggesting modulation of these channels by Ca2+ (23), and the rNav1.2-L1 was shown to interact with synaptotagmin in a Ca2+-regulated manner (46). An interaction with synaptotagmin may modulate sodium channel activity by controlling the accessibility of a protein kinase A phosphorylation site located inside the binding region of this complex or may play a role in the internalization of sodium channels (46). A more intriguing possibility is that this interaction may result in Na+-dependent exocytosis (46).

The TTX-R sodium channel rNav1.9a is preferentially expressed in small diameter C-type sensory neurons of DRG and trigeminal ganglia (14). The expression of rNav1.9a is dynamic and is modulated by the action of neurotrophic factors. Rat Nav1.9a transcripts and protein and the current attributable to rNav1.9a are significantly down-regulated in vivo after axotomy or chronic constriction injury of the sciatic nerve (14, 17, 47) and in an in vitro model of axotomy (47, 48). Expression of rNav1.9a after axotomy is restored by exposure to the neurotrophic factor glial derived neurotrophic factor, but not nerve growth factor (47, 48).

The Nav1.9a channel has unique properties compared with other sodium channels. The Nav1.9a channel is persistent at -60 to -70 mV and shows a significant window current around these potentials (16), thus contributing to setting the resting membrane potential and to subthreshold electrogenesis (18). Heterologous expression of recombinant rNav1.9a in HEK293 mammalian cells or DRG neurons in culture for 7 days after biolistic transformation did not show TTX-R currents similar to those observed in vivo.2 One possibility is that these expression systems may lack a factor(s) that is important for the stability or efficient anchoring of the rNav1.9a channels in the membranes of these cells or the modulation of these channels so that they can produce the predicted Na+ currents. The expression of the Drosophila sodium channel para in heterologous expression systems is dependent upon the co-expression of tipE factor (49).

This study presents evidence from multiple independent assays that demonstrates the direct interaction of rFHF1B with the C-terminal polypeptide of rNav1.9a both in vivo and in vitro. Despite the 50% similarity of the amino acid sequence of the C-terminal polypeptides of sodium channels, rFHF1B did not bind the C termini of hNav1.7 (hNaNE) and rNav1.8a (SNS), the two other sodium channels that are preferentially expressed in DRG neurons. Although the conserved region CR1 of rNav1.9a (aa 1588-1657) is sufficient to bind this factor, the full conserved region CR1+CR2 (aa1588-1726) is needed for efficient binding with rFHF1B. These data clearly demonstrate the specific interaction between the C-terminal polypeptide of rNav1.9a and rFHF1B.

FHFs constitute a distinct branch of the FGF family with significant homology to other FGF members in the central domain but with divergent N and C termini (24). Alternative splicing produces variants of FHFs that contain a nuclear localization signal and others that lack such signals (24, 50-52). All FHF members discovered to date that lack a nuclear localization signal (24, 50-52), like FGF-1 and FGF-2, also lack a canonical signal peptide for secretion via the endoplasmic reticulum/Golgi pathway. Consistent with the lack of signal peptides, recombinant FHFs expressed in HEK293 cells are not secreted into the culture media (24). Although some FHF isoforms can interact with extracellular heparin sulfate proteoglycans, they do not activate FGF receptors nor have they been directly linked to a biological activity (36, 53). The ectopic expression of FHF-2 in chicken limb buds, however, leads to morphological abnormalities, suggesting that this factor play a role in pattern formation (36).

Like other members of the FGF family of growth factors, FHF1B is highly conserved among various species. The similarity at the amino acid level between full-length rat (this study) and chicken (36) FHF1B is 97%. Rat FHF1B is 100 and 97% similar, respectively, to the corresponding sequences that have been reported for mouse and human FHF1B (52). The expression of rFHF1B in adult rat tissue as determined by reverse transcription-PCR (Fig. 2) is similar to its expression pattern in the chicken (36), except for the presence of rFHF1B in adult rat DRG and trigeminal tissue. The expression of FHF1B, however, is more extensive than that of rNav1.9a. Therefore, this factor may interact with other proteins, possibly including other sodium channels. More experiments are needed to determine if rFHF1B interacts with sodium channels that are expressed in the brain.

The lack of a canonical signal peptide and nuclear localization signal in rat and chicken FHF1B suggests that FHF1B may exert its effect via interaction with cytosolic proteins either directly or by acting as a bridge to assemble a functional protein complex. Alternatively, FHF1B may be exported to the extracellular space via an endoplasmic reticulum/Golgi-independent mechanism similar to that responsible for FGF2 export (54), which is inhibited by cardenolides, suggesting a role of the alpha -subunit of Na+/K+-ATPase in this pathway (55, 56). It is not unreasonable to speculate that Nav1.9a may have a function other than the generation of a persistent sodium current in DRG neurons (16) and that it may participate in the export of FHF1B to the cell surface or the exterior of the cell (55, 56), where it might exert its function. Alternatively, the interaction between rFHF1B and the C terminus of rNav1.9a may modulate this channel directly or indirectly by recruiting other proteins to the channel complex.

    ACKNOWLEDGEMENTS

We thank Dr. Joel A. Black and William N. Hormuzdiar for providing DRG cultures, Dr. Ted Cummins for helpful discussions, and Bart Toftness for technical assistance.

    FOOTNOTES

* This work was supported in part by grants from the National Multiple Sclerosis Society and the Rehabilitation Research and Development Service and Medical Research Services, Department of Veterans Affairs and by gifts from the Paralyzed Veterans of America and Eastern Paralyzed Veterans Association.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF348446.

|| Supported by a Spinal Cord Research Fellowship from the Eastern Paralyzed Veterans Association.

** To whom correspondence should be addressed: PVA/EPVA Neuroscience Research Center, Yale University School of Medicine, 127A, Bldg. 34, 950 Campbell Ave., West Haven, CT 06516. Tel.: 203-937-3802; Fax: 203-937-3801; E-mail: sulayman.dib-hajj@yale.edu.

Published, JBC Papers in Press, March 16, 2001, DOI 10.1074/jbc.M101606200

2 S. D. Dib-Hajj, T. R. Cummins, L. Tyrrell, and S. G. Waxman, unpublished information.

    ABBREVIATIONS

The abbreviations used are: DRG, dorsal root ganglia; TTX, tetrodotoxin; TTX-R, tetrodotoxin-resistant; Y2H, yeast two-hybrid screening; FGF, fibroblast growth factor; FHF, FGF homologous factor; GFP, green fluorescent protein; HEK cells, human embryonic kidney cells; aa, amino acids; PCR, polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GST, glutathione S-transferase; PAGE, polyacrylamide gel electrophoresis; DBD, DNA binding domain; AP, activation domain; UR, unique region; CR, conserved region; SD, synthetic dropout base.

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