Hydroxyeicosanoids Bind to and Activate the Low Affinity Leukotriene B4 Receptor, BLT2*

Takehiko YokomizoDagger §, Kazuhiko Kato||, Hiroshi HagiyaDagger , Takashi Izumi§**, and Takao ShimizuDagger §

From the Dagger  Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Tokyo and the § Japan Science and Technology Corporation (CREST), Tokyo 113-0033, Japan, the || Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd., Yokohama 222-8567, Japan, and the ** Department of Biochemistry, Faculty of Medicine, Gunma University, Maebashi 371-8511, Japan

Received for publication, December 18, 2000, and in revised form, January 17, 2001



    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Leukotriene B4, an arachidonate metabolite, is a potent chemoattractant of leukocytes involved in various inflammatory diseases. Two G-protein-coupled receptors for leukotriene B4 have been cloned and characterized. BLT1 (Yokomizo, T., Izumi, T., Chang, K., Takuwa, Y., and Shimizu, T. (1997) Nature 387, 620-624) is a high affinity receptor exclusively expressed in leukocytes, and BLT2 (Yokomizo, T., Kato, K., Terawaki, K., Izumi, T., and Shimizu, T. (2000) J. Exp. Med. 192, 421-432) is a low affinity receptor expressed more ubiquitously. Here we report the binding profiles of various BLT antagonists and eicosanoids to either BLT1 or BLT2 using the membrane fractions of Chinese hamster ovary cells stably expressing the receptor. BLT antagonists are grouped into three classes: BLT1-specific U-75302, BLT2-specific LY255283, and BLT1/BLT2 dual-specific ZK 158252 and CP 195543. We also show that 12(S)-hydroxyeicosatetraenoic acid, 12(S)-hydroperxyeicosatetraenoic acid, and 15(S)-hydroxyeicosatetraenoic acid competed with [3H]LTB4 binding to BLT2, but not BLT1, dose dependently. These eicosanoids also cause calcium mobilization and chemotaxis through BLT2, again in contrast to BLT1. These findings suggest that BLT2 functions as a low affinity receptor, with broader ligand specificity for various eicosanoids, and mediates distinct biological and pathophysiological roles from BLT1.



    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Leukotriene B4 ((5S,12R)-dihydroxy-6,14-cis-8,10-trans-eicosatetraenoic acid (LTB4))1 is a metabolite of arachidonic acid and is one of the most potent activators of granulocytes and macrophages (1-3). BLT, the LTB4-specific G-protein-coupled receptor (GPCR), is a target for anti-inflammatory drugs, and many BLT antagonists have been developed and are being evaluated. No BLT antagonists are yet approved available for clinical use; their lack of efficacy may be due in part to the presence of the other LTB4 receptors. We cloned BLT1, a high affinity LTB4 receptor (4), and BLT2, a low affinity LTB4 receptor (5), and showed that BLT1 is expressed almost exclusively in peripheral leukocytes, whereas BLT2 is expressed ubiquitously with the highest expression in spleen. The structural similarities of these receptors (45% amino acid identity) and the low homologies of BLTs to other known GPCRs suggest that these BLTs comprise a novel receptor family. BLT1 and BLT2 form a gene cluster both in human and mouse genomes. The human BLT2 open reading frame overlaps the promoter region of BLT1 (6), suggesting the expression of these two LTB4 receptors is tightly intertwined. Human granulocytes, eosinophils, and mononuclear cells express both BLT1 and BLT2 (7), so the precise pharmacological characterization of these two receptors using native cells is difficult. In this paper, we report the inhibitory effects of various BLT antagonists and eicosanoids on LTB4 binding using CHO cells stably expressing either human BLT1 or BLT2. We also show that several hydroxyeicosanoids other than LTB4 bind to and activate BLT2 but not BLT1. These findings provide insights into the possible functions of BLT2 and information helpful for the isolation of the other related GPCRs that recognize eicosanoids.


    EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Materials-- U75302 (8) and all of the eicosanoids other than LTB4 were purchased from Cayman Chemical Co. LTB4 is a generous gift from Ono Pharmaceutical Co. (Osaka, Japan). LY 255283 (9) and LY 223982 (10) were from Lilly Research Laboratories. CP 105696 (11) and CP 195543 (12) are from Pfizer Inc. ZK 158252 is from Schering AG (Berlin, Germany). [3H]LTB4 (6956 GBq/mmol) was purchased from PerkinElmer Life Sciences.

Cell Culture and Flow Cytometry-- CHO cells stably expressing FLAG-tagged human BLT1 (CHO-FLAG-BLT1) or hemagglutinin (HA)-tagged human BLT2 (CHO-HA-BLT2) were established as described previously (5, 13). The cells were cloned by limiting dilution and their expression of the receptors confirmed by staining the cells with antibodies against the epitope added to the amino terminus of the receptors. The cells were fixed with PBS(-) containing 0.5% (w/v) paraformaldehyde for 5 min on ice and blocked with PBS(-) containing 2% goat serum (Life Technologies, Inc.). The cells were incubated with 30 µg/ml anti-FLAG antibody (clone M5, Eastman Kodak), 10 µg/ml anti-HA antibody (clone CA12-5, Roche Molecular Biochemicals), or 30 µg/ml control mouse IgG (Santa Cruz Biotechnology) in PBS(-) containing 2% oat serum for 30 min at room temperature, followed by staining with 500 × fluorescein isothiocyanate-labeled anti-mouse IgG (Beckman Coulter, CA) for 30 min at room temperature. The cells were washed twice with PBS(-), and analyzed with flow cytometry, Epics XL (Beckman-Coulter). Cells expressing each receptor were maintained in Ham's F12 medium supplemented with 10% fetal calf serum (Sigma), 0.3 mg/ml G418 (Sigma), 100 IU/ml penicillin, and 100 µg/ml streptomycin.

Binding Assay-- Cells were harvested and sonicated in a buffer containing 20 mM Tris-HCl, pH 7.4, 0.25 M sucrose, 10 mM MgCl2, 2 mM EDTA-Na2, 2 mM phenylmethylsulfonyl fluoride, and 1 µM pepstatin A. After centrifugation at 12,000 × g for 10 min at 4 °C, the remaining supernatants were further centrifuged at 105,000 × g for 60 min. The resulting pellets were used for the binding assay as membrane fractions. The concentrations of the protein were determined by the method of Bradford using a Bio-Rad protein assay. The membrane fractions of CHO cells were examined for [3H]LTB4 binding. The binding mixture (100 µl) contained membrane fractions (20 µg of protein) and 5 nM [3H]LTB4 (34.78 KBq/ml) with/without various concentrations of antagonists or eicosanoids in binding buffer (50 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 10 mM NaCl, and 0.05% BSA). For determination of the nonspecific binding, unlabeled LTB4 was added to the binding mixture to a final concentration of 10 µM. The mixtures were incubated at room temperature for 60 min with gentle agitation followed by rapid filtration through GF/C filters (Packard) and washing with 3 ml of binding buffer. The radioactivity levels of the filters were determined with a Top Count scintillation counter (Packard).

Measurement of Intracellular Calcium Concentration-- CHO cells were loaded with 3 µM Fura-2'AM (Dojin, Kumamoto, Japan) in a modified Hepes-Tyrode's BSA buffer (25 mM Hepes-NaOH, pH 7.4, 140 mM NaCl, 2.7 mM KCl, 1.0 mM CaCl2, 12 mM NaHCO3, 5.6 mM D-glucose, 0.37 mM NaH2PO4, 0.49 mM MgCl2, 0.01% (w/v) cremophore, and 0.1% (w/v) fatty acid-free BSA (Fraction V, Bayer)) at 37 °C for 1 h. The cells were washed twice and resuspended in Hepes-Tyrode's BSA buffer (without cremophore) at a density of 1 × 106 cells/ml. 0.5 ml of the cell suspension was applied to a CAF-100 system (Jasco), and 5 µl of ligand solution was added. The ligands were dissolved in Hepes-Tyrode's BSA buffer after evaporation of the stock solution in ethanol under nitrogen stream and used immediately. Intracellular Ca2+ concentration was measured by the ratio of emission fluorescence of 500 nm by excitation at 340 and 380 nm.

Chemotaxis Assay-- CHO cells expressing hBLT1 or hBLT2 were examined for their chemotactic responses as described previously (4). Briefly, 8 × 104 cells were left to migrate toward the ligands for 4 h through fibronectin-coated filters in a 96-well Boyden chamber. After migration, the cells on the upper side of the filter were wiped off and stained with Diff-Quik reagent, and the migrated cells were quantified by measuring the A595 nm using a microplate reader.

Presentation of the Data-- All figures shown contain representative data from at least two independent experiments with similar results.


    RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Binding Profiles of Various BLT Antagonists to BLT1 and BLT2-- In the light of our knowledge the actions of BLT antagonists that have been developed as anti-inflammatory drugs has to be re-evaluated. Most were characterized using human peripheral leukocytes before the identification of two kinds of LTB4 receptors, BLT1 and BLT2, and their coexpression in peripheral leukocytes (5), monocytes, and eosinophils (7). To distinguish the effects of BLT antagonists on these two receptors, we examined their inhibitory effects on [3H]LTB4 binding to the membrane fractions of CHO cells stably expressing either human BLT1 or BLT2. CHO cells are useful for this purpose because they do not express any intrinsic receptors for LTB4, as revealed by [3H]LTB4 binding and calcium mobilization assays (data not shown). Receptors tagged with FLAG or HA sequences at their amino termini were introduced into the cells, and several clones stably expressing each receptor were isolated. The proper expression of the receptors on the cell surface was confirmed by staining the cells with antibodies specific to the tag, which was followed by flow cytometry (Fig. 1). The membrane fractions of these cells (20 µg of membrane protein) were examined for their binding with 5 nM [3H]LTB4. Fig. 2 shows the competition of 5 nM [3H]LTB4 binding by various BLT antagonists. LTB4 binding to BLT1 was inhibited by CP 105696, ZK 158252, CP 195543, and U75302 in a dose-dependent manner but not by 10 µM LY 255283 or LY 223982. In contrast, LTB4 binding to BLT2 was inhibited by ZK 158252, LY 255283, and CP 195543 but not by 10 µM U75302, CP105695 or LY 223982. We also examined their agonistic activities on these receptors by calcium mobilization and found that 1 and 10 µM U75302 acts as a weak agonist on human BLT1, and 1 and 10 µM CP 195543 acts as a weak agonist on human BLT2 (data not shown).



View larger version (23K):
[in this window]
[in a new window]
 
Fig. 1.   Expression of FLAG-BLT1 or HA-BLT2 in CHO cells. CHO cells were transfected with empty vector (A), FLAG-BLT1 (B), or HA-BLT2 (C), selected with 1 mg/ml G418, and cloned by limiting dilution. The cells were stained with anti-FLAG, anti-HA, or control mouse IgG and fluorescein isothiocyanate-labeled secondary antibody as described under "Experimental Procedures." 10,000 cells were analyzed by flow cytometry.



View larger version (18K):
[in this window]
[in a new window]
 
Fig. 2.   Inhibition of [3H]LTB4 binding to BLT1 or BLT2 by BLT antagonists. 5 nM [3H]LTB4 binding to the membrane preparations (20 µg of membrane protein) from CHO-FLAG-BLT1 (A) and CHO-HA-BLT2 (B) cells was competed with various concentrations of BLT antagonists. Data are represented as % inhibition compared with 10 µM LTB4 as 100%. In CHO-FLAG-BLT1 cells, total binding and nonspecific binding were 62424 ± 563 and 1818 ± 72 dpm, respectively. In CHO-HA-BLT2 cells, total binding and nonspecific binding were 5070 ± 236 and 1374 ± 123 dpm, respectively (n = 3, average ± S.D.).

Binding Profiles of Various Eicosanoids to BLT1 and BLT2-- The membrane fraction of HEK 293 cells transfected with human BLT2 cDNA exhibited a specific and saturable binding for [3H]LTB4 with a Kd value of 23 nM, which is higher than that for human BLT1 by 20-fold (5). Thus, at that time we concluded that BLT2 is a low affinity receptor for LTB4. In the present study, we examined the inhibition by various eicosanoids of [3H]LTB4 binding to the membrane fractions of CHO cells stably expressing BLT1 or BLT2. Fig. 3 shows the inhibition of 5 nM [3H]LTB4 binding by various eicosanoids at a concentration of 5 µM. LTB4 binding to BLT1 is very specific, because only LTB4, 20-hydroxy-LTB4, and 12-epi-LTB4 showed significant competitions at this concentration (Fig. 3A). These results are consistent with our previous results using the membrane fractions of COS-7 cells transiently transfected with BLT1 (4). On the other hand, the binding of LTB4 to BLT2 was inhibited by various eicosanoids (Fig. 3B). 12(R)- and 12(S)-HETE, 12(S)-HPETE, 15(S)-HETE, and 15(S)-HPETE in addition to LTB4, 20-hydroxy LTB4, and 12-epi-LTB4 showed significant inhibition (>50%) of 5 nM [3H]LTB4 binding. Next, we tested various concentrations of LTB4, 20-hydroxy-LTB4, 12(S)-HPETE, 12(S)-HETE, 12(R)-HETE, and 15(S)-HETE in the competition with [3H]LTB4 binding to BLT1 and BLT2. Only LTB4 and 20-hydroxy LTB4 showed dose-dependent inhibitions of LTB4 binding to BLT1 (Fig. 4A). In the case of BLT2, however, all of the eicosanoids tested exhibited dose-dependent inhibition of LTB4, with a rank order of LTB4 > 12(S)-HETE > 12(S)-HPETE > 12(R)-HETE > 15(S)-HETE > 20-hydroxy LTB4 (Fig. 4B). These results clearly show that the recognition of the ligands by BLT1 and BLT2 differs, with more promiscuity in BLT2.



View larger version (28K):
[in this window]
[in a new window]
 
Fig. 3.   Inhibition of [3H]LTB4 binding to BLT1 or BLT2 by eicosanoids. 5 nM [3H]LTB4 binding to the membrane preparations (20 µg of membrane protein) from CHO-FLAG-BLT1 (A) and CHO-HA-BLT2 (B) cells was competed with 5 µM eicosanoids (n = 3, average ± S.D.). ETE, eicosatetraenoic acid; LX, lipoxin.



View larger version (22K):
[in this window]
[in a new window]
 
Fig. 4.   Inhibition of [3H]LTB4 binding to BLT1 or BLT2 by eicosanoids. 5 nM [3H]LTB4 binding to the membrane preparations (20 µg of membrane protein) from CHO-FLAG-BLT1 (A) and CHO-HA-BLT2 (B) cells was competed with various concentrations of eicosanoids (n = 3, average ± S.D.).

Calcium Mobilization by Various Eicosanoids through BLT1 and BLT2-- We next examined whether these eicosanoids could activate intracellular signaling through these two receptors. We used a calcium mobilization assay, because it is a very sensitive and quantitative method for detecting LTB4-BLT interaction. In CHO-FLAG-BLT1 cells, only LTB4 exhibited a robust increase in intracellular calcium concentrations (Fig. 5A). The one exception was seen in case of 12(S)-HPETE, where there was a very slight signal seen at 10 µM (Fig. 5B). In contrast, all of the ligands that were able to bind to BLT2 (Fig. 4B) exhibited significant increases in intracellular calcium concentrations in CHO-HA-BLT2 cells, showing that these eicosanoids are active on BLT2 (Fig. 5, A and B). Fig. 5, C and D, shows dose-response curves of increase in calcium concentrations on CHO-FLAG-BLT1 and CHO-HA-BLT2 cells, respectively. 12(S)-HETE and 15(S)-HETE up to a concentration of 10 µM did not induce measurable changes in intracellular calcium through BLT1 (Fig. 5C). On the other hand, these eicosanoids exhibited dose-dependent increases in calcium concentrations in CHO-HA-BLT2 cells (Fig. 5D). 12-epi-LTB4, a 12(S) epimer of LTB4, acts as a full agonist for BLT1 and BLT2 (Fig. 5, B and C), but a higher concentration of 12-epi-LTB4 is required. These results clearly show that BLT2 is able to bind to various eicosanoids and to transduce intracellular signaling.



View larger version (18K):
[in this window]
[in a new window]
 
Fig. 5.   Calcium mobilization by various eicosanoids through BLT1 or BLT2. A, typical results of calcium mobilizations by various eicosanoids. B, calcium increases in CHO cells by 10 µM eicosanoids (n = 3, average ± S.D.). C and D, dose responses of calcium mobilization by LTB4, 12(S)-HETE, 15(S)-HETE, and 12-epi-LTB4 in CHO-FLAG-BLT1 (C) and CHO-HA-BLT2 (D) cells (n = 3, average ± S.D.).

Chemotactic Response of CHO-BLT2 Cells by Eicosanoids-- As LTB4 is a potent chemoattractant for granulocytes, eosinophils, and macrophages, and CHO cells expressing BLT1 or BLT2 migrate to LTB4 in vitro (4, 5, 7), we asked whether other eicosanoids could evoke chemotactic responses through BLT1 and BLT2. CHO cells expressing BLT1 showed clear chemotactic activities toward very low concentration of LTB4 and high concentration (>10 µM) of 12(R)-HETE and 12(S)-HETE, as shown in Fig. 6A. In the case of BLT2, the optimum concentrations of LTB4 and 12(S)-HETE are close, and the 12(S)-HETE is more effective than 12(R)-HETE (Fig. 6B). The dose-response curves of LTB4 and 12(S)-HETE in CHO-BLT2 cells are bell-shaped as expected in in vitro chemotaxis assays. 12-epi-LTB4 also induced chemotaxis both in CHO-BLT1 and CHO-BLT2 cells, with higher optimum concentrations than LTB4. CHO cells transfected with an empty vector, pcDNA3, as a control did not show any chemotaxis toward LTB4, 12(S)-HETE, 12(R)-HETE, or 12-epi-LTB4 (Fig. 6C).



View larger version (24K):
[in this window]
[in a new window]
 
Fig. 6.   Chemotactic responses of CHO cells toward eicosanoids. CHO cells were left to migrate toward various concentrations of eicosanoids for 4 h in 96-well Boyden chambers. The cells that migrated to the lower side of the filters were quantified by staining with Diff-Quik reagent followed by measuring the A595 nm and represented as the Chemotaxis Index (4). The absolute values of the OD of CHO-FLAG-BLT1, CHO-HA-BLT2, and CHO-vector cells in the absence of the ligands are 0.059 ± 0.0023, 0.0705 ± 0.0005, and 0.0715 ± 0.005, respectively (n = 4, average ± S.E.).



    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

LTB4 is biologically important for the clearance of microorganisms or foreign bodies by activating and recruiting granulocytes to the inflamed lesions (14). Overproduction of LTB4, however, is also involved in various inflammatory diseases including psoriasis (15), bronchial asthma (16), rheumatoid arthritis (17), ulcerative colitis (18), and ischemic reperfusion injury in various tissues (13). Attempts to understand the biological functions of LTB4 and to develop potent and selective BLT antagonists would be assisted by the molecular identification of LTB4 receptors. Several groups including ours have cloned and characterized two distinct LTB4 receptors, BLT1 and BLT2. BLT1 (4, 19-24), a high affinity and leukocyte-restricted LTB4 receptor, is presumably the classical BLT, and most of the BLT antagonists developed thus far are targeted to this receptor. Very recently, BLT2, with a structural similarity to BLT1, was identified as a low affinity receptor for LTB4 (5, 7, 25). One group reported that BLT2 is a high affinity receptor for LTB4 when expressed in Cos-7 cells (26), but the clear explanations for this discrepancy are not available. The BLT2 gene is localized very close to the BLT1 gene both in human and mouse, suggesting that these two receptors are linked genetically (6, 27). BLT2 shows a relatively ubiquitous expression with the highest level in the spleen, followed by the liver, ovary, and peripheral leukocytes (5, 7). Using semiquantitative reverse transcriptase-polymerase chain reaction analysis, BLT1 and BLT2 have been shown to be coexpressed in human granulocytes, mononuclear cells, and eosinophils (7). A number of papers have reported the inhibitory effects of various BLT antagonists on LTB4 binding and various LTB4-induced phenomena, but most of the results were obtained using leukocytes that intrinsically express both BLT1 and BLT2. To correctly understand the pharmacological characters of BLT1 and BLT2, we introduced the expression vectors for these receptors into CHO cells, which do not express any intrinsic LTB4 receptors, and compared the effects of various BLT antagonists and eicosanoids. These receptors are properly expressed on the cell surface as revealed by flow cytometry (Fig. 1). Binding assays using the fractionated cell membrane revealed clear differences in pharmacological profiles of various BLT antagonists on BLT1 and BLT2. CP 105696 and U75302 effectively inhibited [3H]LTB4 binding to BLT1, but they did not inhibit LTB4 binding to BLT2 (Fig. 2), showing that these compounds are specific to BLT1. In addition, U75302 acts as a weak agonist on BLT1, because it increased intracellular calcium in CHO-BLT1 cells (data not shown). On the other hand, LY 255283 inhibited [3H]LTB4 binding to BLT2 but not to BLT1. CP 195543 and ZK 158252 inhibited [3H]LTB4 binding to both receptors, but CP 195543 at 1 µM increased intracellular calcium in CHO-BLT2 cells (data not shown). Thus, CP 195543 acts as an antagonist for BLT1 but a weak agonist for BLT2. Various BLT antagonists have been developed and examined for their effects on inflammatory animal models, but no compounds are available for clinical use. If the expression/function of these two receptors are intertwined, as would be predicted based on their juxtaposition in the genome, a clinically important effect may lie in a compound that has independent effects on each receptor as well as one that blocks both. Our studies point to the need to re-evaluate past studies in light of the independent actions of the compound on each receptor and to find a way to screen future candidate compounds.

We next examined the ligand specificity for BLT1 and BLT2. From initial screenings of various eicosanoids on ligand binding assays (Fig. 3), we selected several hydroxy- or hydroperoxyeicosatetraenoic acids to examine carefully the dose-dependent inhibition of [3H]LTB4 binding to BLT1 and BLT2. To our surprise, some of these eicosanoids effectively inhibited LTB4 binding to BLT2 (Fig. 4). The rank order of potency in binding to BLT2 is LTB4 > 12(S)-HETE > 12(S)-HPETE > 12(R)-HETE > 15(S)-HETE > 20-hydroxy LTB4 (Fig. 4B), which is different from the order of potency in binding to BLT1 (LTB4 > 20-hydroxy-LTB4 12(R)-HETE, Fig. 4A (4)). We next examined the agonistic activities of these eicosanoids using a calcium mobilization assay and found that some of them act as agonists on BLT2 (Fig. 5). Although high concentrations of the ligands are required, CHO-BLT2 cells exhibited clear calcium responses toward 1 µM 12(S)-HETE or 15(S)-HETE. 12(S)-HETE and 12(R)-HETE also induced significant cell migration in CHO-BLT2 cells at lower concentrations than for CHO-BLT1 cells (Fig. 6), suggesting that BLT2 recognizes these eicosanoids and transduces intracellular signaling at physiological concentrations. Despite the broad interests in leukotriene receptors, limited information is available on receptors for HETE and HPETE. Among these receptors, 12(S)-HETE binding sites have been well characterized in human epidermal and carcinoma cells (28-30). High affinity 12(S)-HETE binding sites in human skin were reported, and Kd values for 12(S)-HETE were in the nM range. The rank order of potency in inhibition of [3H]12(S)-HETE binding is 12(S)-HETE > 12(R)-HETE >=  LTB4, which is similar to that of BLT2. In murine B16 melanoma cells, 12(S)-HETE-dependent activation of protein kinase C is mediated by a GPCR and dependent on Gi-like G-protein (31). 12(S)-HETE-binding protein in the cytosolic and nuclear fractions of Lewis lung carcinoma cells was characterized and shown to interact as a heterodimer with steroid receptor coactivator-1 (SRC-1) in the presence of 12(S)-HETE (32). The peroxisome proliferator-activated receptor alpha  (PPARalpha ) was also reported to interact with 12(S)-HETE (33). These results suggest that 12(S)-HETE may possess a dual receptor system, cell surface GPCRs, and intranuclear transcriptional factors. BLT2 can interact with high concentrations of 12(S)-HETE, but the Kd of BLT2 for 12(S)-HETE is higher than the reported high affinity 12(S)-HETE receptor(s) in keratinocytes and melanocytes. The selectivity of BLT2 in the recognition of 12(S)-HETE and 15(S)-HETE and the structural information of BLT2 may be helpful for the identification of the high affinity GPCR for HETEs.

We also showed by calcium mobilization and chemotaxis assays that 12-epi-LTB4 is active both on BLT1 and BLT2. There are no published reports on the in vivo occurrence or biological functions of 12-epi-LTB4, but it is clear that it acts as an agonist for BLT1 and BLT2 in heterologously expressed systems. Further study is required on the biosynthesis and functions of 12-epi-LTB4.

In summary, we have revealed the specificity of various BLT antagonists on two LTB4 receptors and identified the novel activation of BLT2 by various HETEs and HPETEs. These findings will lead to the development of novel BLT antagonists that may be more specific and therefore more potent as anti-inflammatory drugs, also raising the possibility of the identification of other as yet unknown eicosanoid receptors.


    ACKNOWLEDGEMENTS

We thank Dr. D. Wong (The University of Tokyo) for critically reading this manuscript and S. Ishii, N. Uozumi, and M. Taniguchi (The University of Tokyo) for discussions. We also thank Ono Pharmaceutical Company for LTB4 and Pfizer Inc., Lilly Research Laboratories, and Schering AG for BLT antagonists.


    FOOTNOTES

* This work was supported in part by grants-in-aid from the Ministry of Education, Culture, Sports, Science and Technology (Japan) and the Human Science Foundation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Recipient of grants from the Yamanouchi Foundation for Metabolic Disorders, the Uehara Memorial Foundation, and the Cell Science Research Foundation. To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan. Tel.: 81-3-5802-2925; Fax: 81-3-3813-8732; E-mail: yokomizo-tky@umin.ac.jp.

Published, JBC Papers in Press, January 18, 2001, DOI 10.1074/jbc.M011361200


    ABBREVIATIONS

The abbreviations used are: LTB4, leukotriene B4 ((5S,12R)-dihydroxy-6,14-cis-8,10-trans-eicosatetraenoic acid); HETE, hydroxyeicosatetraenoic acid; HPETE, hydroperoxyeicosatetraenoic acid; BSA, bovine serum albumin; GPCR, G-protein-coupled receptor; CHO, Chinese hamster ovary; HA, hemagglutinin; PBS, phosphate-buffered saline.


    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES


1. Samuelsson, B., Dahlen, S. E., Lindgren, J. A., Rouzer, C. A., and Serhan, C. N. (1987) Science 237, 1171-1176[Medline] [Order article via Infotrieve]
2. Serhan, C. N., Haeggstrom, J. Z., and Leslie, C. C. (1996) FASEB J. 10, 1147-1158[Abstract/Free Full Text]
3. Shimizu, T., and Wolfe, L. S. (1990) J. Neurochem. 55, 1-15[Medline] [Order article via Infotrieve]
4. Yokomizo, T., Izumi, T., Chang, K., Takuwa, Y., and Shimizu, T. (1997) Nature 387, 620-624[CrossRef][Medline] [Order article via Infotrieve]
5. Yokomizo, T., Kato, K., Terawaki, K., Izumi, T., and Shimizu, T. (2000) J. Exp. Med. 192, 421-432[Abstract/Free Full Text]
6. Kato, K., Yokomizo, T., Izumi, T., and Shimizu, T. (2000) J. Exp. Med. 192, 413-420[Abstract/Free Full Text]
7. Kamohara, M., Takasaki, J., Matsumoto, M., Saito, T., Ohishi, T., Ishii, H., and Furuichi, K. (2000) J. Biol. Chem. 275, 27000-27004[Abstract/Free Full Text]
8. Falcone, R. C., and Aharony, D. (1991) Eur. J. Pharmacol. 206, 333-338[Medline] [Order article via Infotrieve]
9. Sehmi, R., Rossi, A. G., Kay, A. B., and Cromwell, O. (1992) Immunol. 77, 129-135[Medline] [Order article via Infotrieve]
10. Surette, M. E., Krump, E., Picard, S., and Borgeat, P. (1999) Mol. Pharmacol. 56, 1055-1062[Abstract/Free Full Text]
11. Showell, H. J., Breslow, R., Conklyn, M. J., Hingorani, G. P., and Koch, K. (1996) Br. J. Pharmacol. 117, 1127-1132[Abstract]
12. Showell, H. J., Conklyn, M. J., Alpert, R., Hingorani, G. P., Wright, K. F., Smith, M. A., Stam, E., Salter, E. D., Scampoli, D. N., Meltzer, S., Reiter, L. A., Koch, K., Piscopio, A. D., Cortina, S. R., Lopez-Anaya, A., Pettipher, E. R., Milici, A. J., and Griffiths, R. J. (1998) J. Pharmacol. Exp. Ther. 285, 946-954[Abstract/Free Full Text]
13. Noiri, E., Yokomizo, T., Nakao, A., Izumi, T., Fujita, T., Kimura, S., and Shimizu, T. (2000) Proc. Natl. Aca. Sci. U. S. A. 97, 823-828[Abstract/Free Full Text]
14. Ford-Hutchinson, A., Doig, M. V., Shipley, M. E., and Smith, M. J. (1980) Nature 286, 264-265[Medline] [Order article via Infotrieve]
15. Iversen, L., Kragballe, K., and Ziboh, V. A. (1997) Skin Pharmacol. 10, 169-177[Medline] [Order article via Infotrieve]
16. Turner, C. R., Breslow, R., Conklyn, M. J., Andresen, C. J., Patterson, D. K., Lopez, A. A., Owens, B., Lee, P., Watson, J. W., and Showell, H. J. (1996) J. Clin. Invest. 97, 381-387[Abstract/Free Full Text]
17. Griffiths, R. J., Pettipher, E. R., Koch, K., Farrell, C. A., Breslow, R., Conklyn, M. J., Smith, M. A., Hackman, B. C., Wimberly, D. J., Milici, A. J., et al.. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 517-521[Abstract]
18. Cole, A. T., Pilkington, B. J., McLaughlan, J., Smith, C., Balsitis, M., and Hawkey, C. J. (1996) Gut 39, 248-254[Abstract]
19. Owman, C., Sabirsh, A., Boketoft, A., and Olde, B. (1997) Biochem. Biophys. Res. Commun. 240, 162-166[CrossRef][Medline] [Order article via Infotrieve]
20. Huang, W. W., Garcia-Zepeda, E. A., Sauty, A., Oettgen, H. C., Rothenberg, M. E., and Luster, A. D. (1998) J. Exp. Med. 188, 1063-1074[Abstract/Free Full Text]
21. Martin, V., Ronde, P., Unett, D., Wong, A., Hoffman, T. L., Edinger, A. L., Doms, R. W., and Funk, C. D. (1999) J. Biol. Chem. 274, 8597-8603[Abstract/Free Full Text]
22. Masuda, K., Yokomizo, T., Izumi, T., and Shimizu, T. (1999) Biochem. J. 342, 79-85[CrossRef][Medline] [Order article via Infotrieve]
23. Toda, A., Yokomizo, T., Masuda, K., Nakao, A., Izumi, T., and Shimizu, T. (1999) Biochem. Biophys. Res. Commun. 262, 806-812[CrossRef][Medline] [Order article via Infotrieve]
24. Boie, Y., Stocco, R., Sawyer, N., Greig, G. M., Kargman, S., Slipetz, D. M., O'Neill, G. P., Shimizu, T., Yokomizo, T., Metters, K. M., and Abramovitz, M. (1999) Eur. J. Pharmacol. 380, 203-213[CrossRef][Medline] [Order article via Infotrieve]
25. Tryselius, Y., Nilsson, N. E., Kotarsky, K., Olde, B., and Owman, C. (2000) Biochem. Biophys. Res. Commun. 274, 377-382[CrossRef][Medline] [Order article via Infotrieve]
26. Wang, S., Gustafson, E., Pang, L., Qiao, X., Behan, J., Maguire, M., Bayne, M., and Laz, T. (2000) J. Biol. Chem. 0011006272
27. Nilsson, N. E., Tryselius, Y., and Owman, C. (2000) Biochem. Biophys. Res. Commun. 274, 383-388[CrossRef][Medline] [Order article via Infotrieve]
28. Arenberger, P., Kemeny, L., and Ruzicka, T. (1993) Epithelial Cell Biol. 2, 1-6[Medline] [Order article via Infotrieve]
29. Arenberger, P., Kemeny, L., Rupec, R., Bieber, T., and Ruzicka, T. (1992) Eur. J. Immunol. 22, 2469-2472[Medline] [Order article via Infotrieve]
30. Arenberger, P., Ruzicka, T., and Kemeny, L. (1991) Skin Pharmacol. 4, 272-277[Medline] [Order article via Infotrieve]
31. Liu, B., Khan, W. A., Hannun, Y. A., Timar, J., Taylor, J. D., Lundy, S., Butovich, I., and Honn, K. V. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 9323-9327[Abstract]
32. Kurahashi, Y., Herbertsson, H., Soderstrom, M., Rosenfeld, M. G., and Hammarstrom, S. (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 5779-5783[Abstract/Free Full Text]
33. Forman, B. M., Chen, J., and Evans, R. M. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 4312-4317[Abstract/Free Full Text]


Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.