A Novel Type of Iron Hydrogenase in the Green Alga Scenedesmus obliquus Is Linked to the Photosynthetic Electron Transport Chain*

Lore Florin, Anestis Tsokoglou, and Thomas HappeDagger

From the Botanisches Institut der Universität Bonn, Karlrobert-Kreiten-Strasse 13, 53115 Bonn, Germany

Received for publication, September 15, 2000, and in revised form, November 20, 2000



    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Hydrogen evolution is observed in the green alga Scenedesmus obliquus after a phase of anaerobic adaptation. In this study we report the biochemical and genetical characterization of a new type of iron hydrogenase (HydA) in this photosynthetic organism. The monomeric enzyme has a molecular mass of 44.5 kDa. The complete hydA cDNA of 2609 base pairs comprises an open reading frame encoding a polypeptide of 448 amino acids. The protein contains a short transit peptide that routes the nucleus encoded hydrogenase to the chloroplast. Antibodies raised against the iron hydrogenase from Chlamydomonas reinhardtii react with both the isolated and in Escherichia coli overexpressed protein of S. obliquus as shown by Western blotting. By analyzing 5 kilobases of the genomic DNA, the transcription initiation site and five introns within hydA were revealed. Northern experiments suggest that hydA transcription is induced during anaerobic incubation. Alignments of S. obliquus HydA with known iron hydrogenases and sequencing of the N terminus of the purified protein confirm that HydA belongs to the class of iron hydrogenases. The C terminus of the enzyme including the catalytic site (H cluster) reveals a high degree of identity to iron hydrogenases. However, the lack of additional Fe-S clusters in the N-terminal domain indicates a novel pathway of electron transfer. Inhibitor experiments show that the ferredoxin PetF functions as natural electron donor linking the enzyme to the photosynthetic electron transport chain. PetF probably binds to the hydrogenase through electrostatic interactions.



    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Many prokaryotes and several eukaryotes have an enzyme complex in common catalyzing the reversible reduction of protons to molecular hydrogen. The diverse group of hydrogenases can be divided into three classes according to their metal composition in the active center (1). The nickel-iron hydrogenases are widespread among all bacteria families and have been well characterized during the last 30 years (2). The iron sulfur proteins consist of one to four subunits and have an additional nickel atom in the catalytic site (3, 4). In contrast, the iron hydrogenases possess only [Fe-S] clusters and an iron cofactor with an unique structure of six iron atoms (5, 6). The third class of hydrogenases lacks the iron sulfur clusters as well as additional metal atoms and was found only in methanogenic bacteria (7, 8).

Until now, iron hydrogenases have only been found in hydrogen-producing anaerobic bacteria and protozoa (9-13). The enzymes allow fermentative anaerobes to evolve H2 without exogenous electron acceptors other than protons (14). They show a high specific activity that is about 100 fold higher compared with the nickel-iron hydrogenases (15). Furthermore, all iron hydrogenases are extremely sensitive to oxygen and carbon monoxide. The structures of the iron hydrogenases from Clostridium pasteurianum and Desulfovibrio desulfuricans have recently been investigated by x-ray crystallography (16, 17). The proteins consist of one or two subunits and have a remarkable iron cofactor (H cluster) in the catalytic site. The H cluster contains an unusual supercluster comprising a [4Fe4S] subcluster and a [2Fe] center, which are bridged together by a single cysteinyl sulfur (18). A number of conserved amino acids forms a hydrophobic pocket that shields the [2Fe] subcluster from the solvent. In all known iron hydrogenases at least eight conserved cysteines were found at the N-terminal site of the protein that coordinate two further [4Fe-4S] clusters (F cluster). It is discussed that the F clusters are responsible for the electron transfer from the surface of the protein to the active site (17, 19).

In green algae, Gaffron (20) discovered a hydrogen metabolism 60 years ago. After anaerobic adaptation, he observed both H2 uptake and hydrogen evolution dependent on the CO2 partial pressure (21, 22). After bubbling the cells with an inert gas like argon, high rates of H2 production can be measured in the light (23). Electrons are supplied either by photochemical water splitting at photosystem II, which results in simultaneous production of hydrogen and oxygen, or by metabolic oxidation of organic compounds with release of CO2 (24-27). Light-dependent electron transport from organic substrate through the plastoquinone pool to the hydrogenase provides the cells with ATP under anaerobic conditions (28, 29).

From the unicellular green alga Chlamydomonas reinhardtii a monomeric iron hydrogenase with high specific activity has been isolated (30, 31). In contrast, a nickel-iron hydrogenase was described for another well examined green alga, Scenedesmus obliquus (32, 33). The protein consists of two subunits of about 36 and 55 kDa and might be located in the chloroplast.

To investigate whether hydrogenases of the iron-only type also occur in green algae other than C. reinhardtii, we decided to look for the gene of a hydrogenase in S. obliquus. Interestingly, we isolated the protein and the gene encoding a monomeric iron hydrogenase (HydA). Although the H cluster of the HydA protein of S. obliquus is very conserved, the N-terminal site is completely different compared with other iron hydrogenases. Further cysteines are not present. These cysteine residues coordinate the typical F clusters that are necessary for the electron pathway in other iron hydrogenases. We performed physiological measurements of the hydrogen evolving activity in the present of chloroplast ferredoxin specific inhibitors as well as antibodies against this protein. The results clearly indicated that ferredoxin transfers electrons to the hydrogenase and links the enzyme to photosynthesis. The expression of the hydA gene is regulated at the transcriptional level. The mRNA is transcribed very rapidly during the process of anaerobic adaptation.


    EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Algae Strains, Growth, and Anaerobic Conditions-- Wild-type S. obliquus Kützing 276-6 was obtained originally from the culture collection of algae at the University of Göttingen. Cells were cultured photoheterotrophically (34) in batch cultures at 25 °C under continuous irradiance of 150 µmol photons × m-2 s-1. For anaerobic adaptation, 4-liter cultures were bubbled vigorously with air supplemented with 5% CO2. After harvesting the cells in the mid exponential stage of growth, the pellet was resuspended in fresh Tris acetate phosphate medium. The algae were anaerobically adapted by flushing the culture with argon in the dark.

Hydrogen Evolution Assay-- The in vitro hydrogenase activity was measured by using a gas chromatograph from Hewlett Packard (HP 5890, Series II) equipped with a thermal conductivity detector and a molecular sieve column. Methylviologen reduced by sodium dithionite was used as electron donor as described before (30). 1 unit is defined as the amount of hydrogenase evolving 1 µmol H2 × min-1 at 25 °C.

The in vivo activity in the presence of different inhibitors of the photosynthetic electron flow was determined as described (30). After anaerobic adaptation, cells were harvested, diluted in fresh Tris acetate phosphate medium, and transferred to sealed tubes. Inhibitors were added 1 h before H2 evolving activity was measured. Cells were broken by sonification. Thylakoid membranes and photosynthetic electron transport chain remained intact as shown by O2 polarography. Ferredoxin of C. reinhardtii and S. obliquus was isolated according to the method of Schmitter et al. (35).

Rapid Amplification of cDNA Ends-Polymerase Chain Reaction-- RACE-PCR (36)1 was performed with the SMARTTMRACE cDNA Amplification Kit (CLONTECH Laboratories, Palo Alto, CA) according to the manufacturer's recommendations except for modifications of the PCR and hybridization conditions. Starting material consisted of 1 µg of mRNA from anaerobically adapted cells. The reverse transcription reaction was carried out with a Moloney murine leukemia virus reverse transcriptase in two separate reaction tubes containing either the 5'- or the 3'-RACE-PCR specific primer from the kit. The cDNA of each sample served as template for the following PCR. For the 5'-RACE-PCR, Universal Primer Mix (CLONTECH) and the antisense primer Sc7 were used. The amplification of the 3'-cDNA end was performed with Universal Primer Mix and the sense primer Sc6. To obtain more distinct PCR signals, the PCR was repeated for both reactions with nested universal primers and designed primers (inverse Sc6 and inverse Sc7, respectively) using a dilution of the products of the first PCR as template.

Primer Extension-- RACE-PCR was also implemented to map the transcription initiation site of the hydA mRNA (37). A gene-specific primer (Sc17) was used to carry out the first strand cDNA synthesis with the Supercript II reverse transcriptase (Life Technologies, Inc.) and 200 ng of mRNA as template. PCR was performed using either Sc12 or Sc27 and the SMARTTM specific adapter primer Universal Primer Mix. Two different DNA fragments of 234 and 183 bp were amplified under standard PCR conditions. Both fragments were cloned into the pGem®T-Easy vector (Promega, Madison, WI) and sequenced using primers from the polylinker of the vector.

Genome Walking with Genomic DNA-- Applying the CLONTECH Genome Walker Kit, genomic libraries from S. obliquus were generated by digestion with different blunt end cutting endonucleases (NaeI, DraI, PvuII, HincII, and EcoRV) and by adapter ligation at the ends of the resulting DNA fragments. These libraries were utilized as independent templates in five different PCR reactions (38). Two gene-specific primers (Sc27 and Sc35) derived from the hydA cDNA sequence of S. obliquus were used in combination with a kit adapter primer (AP1) in a first PCR reaction. Subsequently, 1 µl of the first PCR served as a template in a secondary PCR, applying two nested gene-specific primers (i-Sc10 and Sc32) along with a nested kit adapter primer (AP2). The resulting products were cloned into pGem®T-Easy and sequenced. Sequencing was performed by the dideoxy chain termination method (39).

Purification of the Iron Hydrogenase-- 40-liter cultures of S. obliquus were grown heterotrophically. After centrifugation (10 min at 5000 × g), the pellet was resuspended in 200 ml of Tris acetate phosphate medium. The cells were anaerobically adapted by flushing the solution with argon for 1 h in the dark. All further purification steps were performed in an anaerobic chamber (Coylab, Ann Arbor, MI). The cells were disrupted in a 50 mM Tris/HCl buffer, pH 8.0, 10 mM sodium dithionite by vortexing 3 min with glass beads. The further purification steps were made as described earlier for the isolation of the iron hydrogenase of C. reinhardtii (30). Automated Edman degradation of the N-terminal site of the protein was performed with an Applied Biosystem model 477A sequencer with online analyzer model 120 A.

RNA Blot Hybridization-- Total RNA of S. obliquus was isolated according to the method described earlier (40). Equal amounts (20 µg) were separated electrophoretically on 1.2% agarose gels containing formaldehyde (41). The RNA was transferred onto nylon membranes (Hybond+, Amersham Pharmacia Biotech) and hybridized with RNA probes labeled with DIG-dUTP using the in vitro transcription method. A 1.3-kilobase EcoRI cDNA fragment was used to detect transcripts of the hydA gene, whereas a DIG-dUTP-labeled cDNA encoding constitutively expressed plastocyanin (42) was used as control. Hybridization reactions were carried out using protocols supplied by the manufacturer (Roche Molecular Biochemicals).

Sequence Analysis Software-- Nucleic acid and protein sequences were analyzed with the programs Sci Ed Central (Scientific Educational Software) and ClustalW (43). The Blast server (44) of the National Center for Biotechnology Information (Bethseda, MD) was used for data base searches.

Recombinant Expression in Escherichia coli-- The hydA open reading frame was amplified by PCR using the primer pair Sc29 and Sc30 containing flanking NdeI-BamHI sites. The PCR product was cloned into the pGem®T-Easy vector. After digestion with NdeI-BamHI, the hydA gene was cloned into the corresponding site of the pET9a expression vector (Promega) producing pLF29.2. The insert of pLF29.2 was sequenced confirming that the fragment contains the exact full coding region of the hydrogenase without transit peptide. E. coli strain BL21(DE3)pLysS was transformed with pLF29.2. Expression was induced with 1 mM isopropyl-thio-beta -D-galactoside at an A600 of 0.3. Pelleted cells were resuspended in lysis buffer (100 mM Tris/HCl, 4 mM EDTA, 16% glycine, 2% SDS, 2% mercaptoethanol, 0.05% bromphenol blue, 8 M urea). After heating, the protein extract was separated by 10% SDS-PAGE and blotted onto a polyvinylidene difluoride membrane. Western blot analyses were performed using antiserum against the iron hydrogenase of C. reinhardtii at 1:1000 dilution as described earlier (31).


    RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Induction of Hydrogenase Activity and Purification of the Iron Hydrogenase Protein-- Anaerobic adaptation is the most efficient way to induce hydrogenase activity in S. obliquus. Bubbling the alga culture in the dark with argon led to a dramatic increase (10-fold) of hydrogenase activity during the first 2 h. We purified the enzyme of S. obliquus to homogeneity by successive column chromatography. Because the enzyme is irreversible inactivated by lowest oxygen levels, all purification steps were performed under strictly anaerobic conditions and in the presence of reducing agents (dithionite). The purification scheme resulted in a 5200-fold purification of HydA with 5% recovery (data not shown). The most powerful step for purifying the protein was a Q-Sepharose high performance column chromatography with pH gradient elution. Gel filtration chromatography of hydrogenase on a calibrated Superdex-75 column resulted in a single activity peak corresponding to a molecular mass of 45 kDa. The monomeric structure of the enzyme could also be shown on a SDS-polyacrylamide gel after Coomassie Blue staining (data not shown). The N-terminal sequence of HydA was determined by Edman degradation. The protein sequence (AGPTAECDRPPAPAPKAXHWQ) is, except for two amino acids, identical to the amino acid sequence deduced from the DNA data (AGPTAECDCPPAPAPKAPHWQ). In the course of our purification procedure, we never found a hint for a second hydrogenase in S. obliquus because the hydrogenase activity was never separated in several distinct fractions. Biochemical data show a high similarity of HydA to the iron hydrogenase from C. reinhardtii (Table I). The enzymes have a high temperature optimum of about 60 °C, are strongly inhibited by O2 and CO, and catalyze the H2 evolution with a typical high specific activity. Experiments with inhibitors of translation on ribosomes (data not shown) and analysis of the gene structure (see below) show that HydA from S. obliquus is translated in the cytoplasm and then transported into the chloroplast.


                              
View this table:
[in this window]
[in a new window]
 
Table I
Biochemical data comparison of purified iron hydrogenases from C. reinhardtii and S. obliquus

Ferredoxin Is the Natural Electron Donor of the Iron Hydrogenase-- Hydrogenase activity was determined in intact and broken cells after anaerobic adaptation. The integrity of the photosynthetic electron transport in the sonified cell preparation was demonstrated by the rate of oxygen evolution (154 µmol O2/mg Chl × h). This rate corresponds to 85% of the oxygen evolution measured with intact Scenedesmus cells.

In S. obliquus, the hydrogen evolution is linked to the photosynthetic electron transport chain through PSI. As shown in Table II, the cells were still able to photoproduce hydrogen when electron flow of PSII was blocked by 3-(3,4-dichlorophenyl)-1,1-dimethylurea. In contrast, addition of 2,5-dibromo-3-methyl-6-isopropyle-p-benzochinone resulted in inhibition of the H2 production, thus giving evidence of the involvement of PSI in the supply of electrons to hydrogenase. The electron transport from PSI to ferredoxin was inhibited using the artificial electron acceptor 2,6-dichlorophenolindophenol. In this reaction, 2,6-dichlorophenolindophenol is reduced instead of ferredoxin, and the electron transfer to hydrogenase is interrupted.


                              
View this table:
[in this window]
[in a new window]
 
Table II
Effects of different photosynthetical inhibitors on hydrogenase activity
After anaerobic adaptation, cells were harvested, diluted in fresh TAP medium, and incubated with inhibitors as described under "Experimental Procedures." alpha -PetF-antibody was raised against spinach ferredoxin. DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethylurea; DBMIB, 2,5-dibromo-3-methyl-6-isopropyle-p-benzochinone; Sulfo-DSPD, sulfo-disalicylidinepropanediamin; DCPIP, 2,6-dichlorophenolindophenol.

Hydrogenase activity was dramatically reduced (up to 30-fold) by the ferredoxin antagonist sulfo-disalicylidinepropandiamine (Table II). Similar results were achieved with alpha -PetF-antibodies that specifically recognize the ferredoxin protein. In both cases, the hydrogenase enzyme can not evolve hydrogen, thus demonstrating the role of ferredoxin as the obligatory electron donor for the hydrogenase reaction.

The electron transfer properties of different plant-type ferredoxins were measured in vitro with dithionite as reducing reagent. The ferredoxin proteins of spinach, C. reinhardtii, and S. obliquus were comparable regarding their capability to reduce purified S. obliquus hydrogenase. In this assay, we obtained H2-evolving activities of 420, 390, and 350 units/mg protein with S. obliquus, C. reinhardtii, and spinach ferredoxin, respectively. No hydrogen production could be measured with other possible electron donors like cytochrome and NADPH. In D. desulfuricans the iron hydrogenase was reported to catalyze both hydrogen production and uptake with low potential multiheme cytochromes like cytochrome c3 (17).

Molecular Characterization of hydA Encoding an Iron Hydrogenase-- To isolate the gene encoding a iron hydrogenase in S. obliquus, we isolated poly(A)+ RNA from cell cultures after 1 h of anaerobic adaptation. Isolated RNA was transcribed and amplified by reverse transcription-PCR using oligonucleotides derived from conserved regions within the C. reinhardtii hydA gene.2 The complete cDNA clone of 2609 bp was obtained by 5'- and 3'-RACE PCR. It contains an open reading frame of 1344 bp encoding a polypeptide of 448 amino acids (Fig. 1) followed by an extensive 3'-untranslated region of about 1100 bp. The coding region of S. obliquus hydA exhibits features common to other green algae such as high GC content (64.2%) and a characteristic putative polyadenylation signal, TGTAA, 15 bp upstream of the poly(A)+ sequence (45).



View larger version (16K):
[in this window]
[in a new window]
 
Fig. 1.   Schematic representation of S. obliquus hydA genomic and cDNA structures. A, the coding region of the hydA cDNA is illustrated as a large arrow with the transit peptide shown in black. The untranslated 5' and 3' sequences are marked as lines. The arrows below indicate the sequencing strategy; each arrow represents an independent sequence determination. TSP, transcription start point; ATG, start codon. B, the mosaic structure of hydA is indicated by gray (exons) and white boxes (introns). The S2 probe and different restriction enzymes that were used in the Southern blot experiments are mentioned.

In an effort to examine the exon-intron structure and the promoter region of the hydA gene, about 5 kilobases of the genomic DNA from S. obliquus were sequenced. The gene comprises five introns with a total size of 1310 bp (Fig. 1) whose 5' and 3' ends contain typical plant splice donor and acceptor sites that follow the GT/AG rule.

A genomic Southern blot was probed with a 750-bp PCR fragment to determine the copy number of the hydA gene (Fig. 2). Single bands were observed in lanes with samples digested with HincII, EcoRV, and NdeI and a double band in the lane containing genomic DNA digested with SacI. The band migration positions matched the sizes predicted from the sequence of the hydA gene, indicating that HydA is encoded by a single copy gene (Fig. 2). The same hybridization pattern was observed even under low stringency conditions (hybridization temperature 50 °C; data not shown). The transcription start position was determined by primer extension using RACE-PCR and was found 139 bp upstream of the ATG start codon. We designed several primers within 100 bp of the 5' end of the known hydA cDNA to confirm the accuracy of the transcription initiation site. All of the sequenced PCR clones had the same 5' ends at position +1. As described for other green algae genes, a highly conserved TATA box element upstream of the transcription starting point is absent (46). However, the TACATAT motive at position -25 in a GC-rich region shows similarities to other TATA motives in C. reinhardtii and therefore might be involved in gene expression.



View larger version (85K):
[in this window]
[in a new window]
 
Fig. 2.   The hydA gene is located in the genome of S. obliquus as a single copy gene. Southern analysis was carried out by digesting genomic DNA of S. obliquus with four different restriction endonucleases (SacI, HincII, EcoRV, and NdeI). 10 µg of DNA was loaded per lane. The S2 DNA-Probe (750 bp) was used for the hybridization as indicated in Fig. 1.

HydA Is a Novel Type of Iron Hydrogenase-- The polypeptide derived from the cDNA sequence has a length of 448 amino acids and a predicted molecular mass of 48.5 kDa (44.5 kDa without transit peptide, respectively); consequently, HydA is the smallest hydrogenase protein known so far. The N terminus of HydA is basic and contains numerous hydroxylated amino acids and an Val-Xaa-Ala motive at position 35, a characteristic feature of chloroplast transit peptides (47, 48).

The processed HydA protein is compared with four bacterial and two eukaryotic iron hydrogenases as shown in Fig. 3. The homology in the C-terminal region of all proteins is quite striking. For example, the S. obliquus HydA protein shows 44% identity and 57% similarity to the C. pasteurianum iron hydrogenase (9). The H cluster in S. obliquus might be coordinated by four cysteine residues at positions 120, 175, 335, and 340. Other strictly conserved amino acid structures like FTSCCPGW (343), TGGVMEAALR (474), and MACPGGCXXGGGQP () probably define a pocket surrounding the active center as shown by the structural data of C. pasteurianum and D. desulfuricans (16, 17). On the other hand, the N-terminal region is completely different from all other iron hydrogenases. The protein sequences of the other enzymes comprise at least two [4Fe-4S] ferredoxin-like domains (called F cluster) that are necessary for the electron transport from the electron donor to the catalytic center. The iron hydrogenases of C. pasteurianum, Thermotoga maritima, and Nyctotherus ovalis (9, 12, 11) contain an extra [4Fe-4S] cluster and one [2Fe-2S] center. This N-terminal domain with the F cluster or other [Fe-S] centers is completely lacking in HydA of S. obliquus. This indicates that there is a direct electron transport pathway from the exogenous donor to the H cluster.



View larger version (118K):
[in this window]
[in a new window]
 
Fig. 3.   Comparison of the deduced HydA protein sequence with other iron hydrogenases. The protein alignment was done by using the Vector NTI program (InforMax). White letters with black background indicate amino acids identical to the HydA protein. Black letters with gray background indicate conserved changes of the amino acids. S. o., S. obliquus (this work); M. e., Megasphaera elsdenii (13); D. d., D. desulfuricans (17); T. v., Trichomonas vaginalis (10); C. p., C. pasteurianum (9); T. m., T. maritima (12); N. o., N. ovalis (11).

To verify that the isolated cDNA encodes a iron hydrogenase, the hydA clone was expressed in the heterologous system E. coli. One band of recombinant HydA protein was observed on SDS-PAGE at ~44 kDa, in agreement with the molecular mass of the polypeptide predicted from the cDNA sequence. Antibodies raised against the HydA protein of C. reinhardtii, which cross-react with other iron hydrogenases but not with nickel-iron hydrogenases (data not shown), were applied in Western blot analysis. One distinct signal with the overexpressed HydA protein of S. obliquus was obtained (Fig. 4).



View larger version (74K):
[in this window]
[in a new window]
 
Fig. 4.   Recombinant expressed HydA reacts with antibodies raised against C. reinhardtii iron hydrogenase. The hydA gene coding region corresponding to amino acid 36 to residue 448 was cloned NdeI-BamHI into pET9a. HydA protein was expressed upon induction with IPTG. Lane 1, recombinant expressed HydA protein from S. obliquus; lane 2, recombinant expressed iron hydrogenase from C. reinhardtii; lane 3, purified hydrogenase from C. reinhardtii; lane 4, total proteins from induced E. coli cells without plasmid. A, SDS-PAGE. Lane M, molecular mass marker (Bio-Rad) indicating relative molecular masses in kDa. SDS-PAGE stained with Coomassie Blue is shown. B, Western blot probed with HydA antibody. The recombinant proteins of lanes 1 and 2 from A were diluted 1:10.

The lysate of induced E. coli cells exhibited no hydrogenase activity. This result corresponds to observations by Voordouw et al. (50) and Stokkermans et al. (51), who also detected no H2 production of recombinant iron hydrogenases in E. coli cells. The reason for that might be that the bacterial cells do not have the ability to assemble the special H cluster of iron hydrogenases.

Rapid Induction of hydA mRNA during Anaerobic Adaptation-- The regulation of the hydA gene expression was examined by Northern blot analysis and reverse transcription-PCR. Aerobically grown cells of S. obliquus did not show a hydrogenase activity (Fig. 5A). Total RNA and also mRNA were isolated from cells that were induced by argon bubbling for 0, 1, and 4 h. Northern blot analysis and reverse transcription-PCR demonstrated that the hydA gene is expressed after anaerobic adaptation. There is a very weak signal without adaptation (t = 0), but strong signals of the transcript could be detected after anaerobic induction (Fig. 5, B and C). The full length of the hydA cDNA clone was confirmed by the transcript signal (2.6 kilobases) on the Northern blot.



View larger version (28K):
[in this window]
[in a new window]
 
Fig. 5.   Induction of the hydrogenase activity and differential expression of the hydA gene during anaerobic adaptation. A, S. obliquus cells were anaerobically adapted by flushing the culture with argon in the dark. After removing cell samples at the indicated times, the algae were broken by Triton X-100 treatment. The in vitro hydrogenase activity was measured as described under "Experimental Procedures." B, Northern hybridization was performed with the hydA-specific probe. Adapted cells were harvested at 0, 1, and 4 h, and the RNA was isolated. 20 µg of total RNA was loaded per lane. C, the same RNA was hybridized with a constitutive expressed gene (plastocyanin) as control.



    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

In green algae, the occurrence of a hydrogen metabolism induced by anaerobic conditions is well established. Despite the great interest in hydrogen evolution for practical applications ("biophotolysis"), the hydrogenase genes from green algae have not yet been isolated. The hydA gene and the isolated HydA protein of S. obliquus that we present in this work belong to the class of iron hydrogenases.

Iron hydrogenases have been isolated only from certain anaerobic bacteria and some anaerobic eukaryotes as well as from the anaerobically adapted green alga C. reinhardtii (30). The enzymes are found to exist in monomeric (9, 13, 53, 54), dimeric (17), and multimeric (12) forms; however, in eukaryotes only monomeric proteins have been isolated (10, 11).

The HydA protein of S. obliquus is synthesized in the cytoplasm. The first 35 residues (Met1-Ala35) of the amino acid sequence derived from the cDNA sequence are supposed to function as a short transit peptide that routes the nuclear encoded protein to the chloroplast. Several positively charged amino acids that describe a typical feature for algal transit peptides (47) are found in HydA. The three terminal residues of the signal sequence, Val-Xaa-Ala, constitute the consensus sequence for stromal peptidases (48).

The hydrogenase of S. obliquus represents a novel type of iron hydrogenase. The monomeric enzyme of 448 amino acids and a calculated molecular mass of 44.5 kDa for the processed protein is the smallest iron hydrogenase isolated so far. The protein sequence consists of an unusual N-terminal domain and a large C-terminal domain containing the catalytic site. The structurally important C terminus of the S. obliquus HydA sequence is very similar to that of other iron hydrogenases. Four cysteine residues at positions 120, 175, 336, and 340 coordinate the special [6Fe] cluster (H cluster) of the active site (Fig. 6). A number of additional residues define the environment of the catalytic center. Peters et al. (16) postulated 12 amino acids in C. pasteurianum to form a hydrophobic pocket around the cofactor. Ten residues are strictly conserved, while two amino acids vary within the iron hydrogenase family (Ser232 and Ile268 in C. pasteurianum, Ala119 and Thr155 in T. vaginalis, and Ala44 and Thr80 in S. obliquus). A small insertion of 16 amino acids is noted in S. obliquus, but this addition occurs in an external loop of the protein and probably has no special function (Fig. 6).



View larger version (29K):
[in this window]
[in a new window]
 
Fig. 6.   Schematic alignment of the conserved cysteine residues and other important amino acids of the H cluster. The protein is illustrated as a large gray arrow. Small arrows indicate parallelograms which demonstrate conserved amino acids in the protein. Cysteines participating at the coordination of the [Fe-S] clusters are gray, whereas identical amino acids are black. An insertion of 16 amino acids in the S. obliquus protein is illustrated as a spotted bar. FS4, [4Fe-4S] cluster; FS2, [2Fe-2S] cluster.

Until now, all iron hydrogenases possessed a ferredoxin-like domain in the N terminus coordinating two [4Fe4S] clusters (FS4A and FS4B; Refs. 10 and 13 and Fig. 6). The iron sulfur clusters facilitate the transfer of electrons between external electron donors or acceptors and the H cluster. The N terminus of the S. obliquus protein is strongly reduced compared with other iron hydrogenases, and no conserved cysteines are found. Therefore, we postulate that all accessory Fe-S clusters (FS2, FS4A, FS4B, and FS4C) are missing. No hints for a second subunit have been observed during purification of the protein.

In contrast to earlier observations in S. obliquus (32), we could neither detect the postulated two subunits of a potential nickel-iron hydrogenase nor find a nickel dependence related to the hydrogenase activity. Francis reported about two forms of hydrogenases in S. obliquus (52), but although we used the same alga strain and identical adaptation conditions, we were not able to detect a second hydrogenase activity during the purification steps.

Physiological studies have shown that the hydrogen evolution is coupled to the light reaction of the photosynthesis (24-26). In contrast to earlier observations in S. obliquus (25, 26), we measured PSII independent H2 production that is not influenced by 3-(3,4-dichlorophenyl)-1,1-dimethylurea. The electrons required for H2 evolution come from redox equivalents of the fermentative metabolism and are supplied into the photosynthetic electron transport chain via the plastochinone pool.

For the first time we demonstrate that the ferredoxin PetF functions as the in vivo electron donor of the iron hydrogenase from S. obliquus. Hydrogenase activity can be specifically blocked by addition of the ferredoxin antagonist sulfo-disalicylidinepropandiamine (55) and antibodies raised against the PetF protein. In vitro, a hydrogen evolution by HydA was only measured with plant-type [2Fe-2S] ferredoxins like PetF of S. obliquus, C. reinhardtii, and spinach as electron mediators. Bacterial iron hydrogenases are known to be reduced by [4Fe-4S] ferredoxins and do not accept electrons from plant-type proteins (56).

The analysis of the three-dimensional structure of the iron hydrogenase from C. pasteurianum (CpI) gave evidence that the interaction with external electron donors might occur at the accessory [Fe-S] clusters in the N-terminal domain (14). Based on the x-ray structure of CpI, we modeled the iron hydrogenase of S. obliquus (57). As shown in Fig. 7, a region of positive surface potential is observed within HydA based on a local concentration of basic residues. In contrast to the docking position of ferredoxin in CpI, these charged amino acids in the S. obliquus iron hydrogenase are located within the C-terminal domain, forming a niche for electron donor fixation.



View larger version (31K):
[in this window]
[in a new window]
 
Fig. 7.   Schematic view of the structures of S. obliquus HydA (A) and the electron donor ferredoxin (B). The figure shows the alpha  carbons and the side chains of charged residues that might be important for the electron transfer reaction or the interaction between HydA and the ferredoxin from S. vacuolatus (58). The 16-amino acid insertion of the hydrogenase appears as external loop and is distinguished as dotted line. The amino acid sequence of the mature HydA protein (His19-Tyr404) was submitted to the SWISS-MODEL server (59). We generated a model of HydA with the known three-dimensional structure of the iron hydrogenase from C. pasteurianum (16) as template, sharing 57% sequence identity with the submitted sequence. The Protein Data Bank file was visualized by the Swiss-PDB viewer (57).

The known algal ferredoxin proteins exhibit high degrees of sequence identity (over 85%), and the charged amino acids are strictly conserved. The petF sequence of S. obliquus is unknown, but very recently the x-ray model of the ferredoxin from another Scenedesmus species (Scenedesmus vacuolatus; Ref. 58) was published. The structure revealed negatively charged amino acids like aspartate and glutamate near the [2Fe-2S] cluster. The [Fe-S] center and the H cluster of the hydrogenase probably come into close proximity through electrostatic interactions. This geometry is consistent with efficient electron transfer among these prosthetic groups.

As already shown in various studies, a correlation exists between the duration of time of the anaerobic adaptation and the increase of hydrogen production (30, 32). Reverse transcription-PCR and Northern blot analyses with mRNA of aerobic and anaerobically adapted cells from S. obliquus showed an increased level of hydA transcript after 1 h of induction. Correspondingly, hydrogen evolution was only measured after a short time of anaerobic adaptation. These results suggest that the expression of the hydA gene is regulated at the transcriptional level. The small amount of transcript that was detected at t = 0 may be due to transcript synthesis induced by microanaerobic conditions during the RNA isolation procedure. Alternatively, a low level of hydA transcript might be constitutively present in the cell and is only drastically increased after anaerobic adaptation.


    ACKNOWLEDGEMENTS

We thank Dr. W. Hachtel for critical reading and A. Kaminski for providing us with sequence information before publication.


    FOOTNOTES

* This work was supported by Deutsche Forschungsgemeinschaft Grant Ha2555/1-1.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

This paper is dedicated to Jan Vlcek who found evidence for an iron hydrogenase in S. obliquus for the first time. Unfortunately, he died 4 years ago in a tragic car accident.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AJ271546.

Dagger To whom correspondence should be addressed: E-mail: t.happe@ uni-bonn.de.

Published, JBC Papers in Press, November 28, 2000, DOI 10.1074/jbc.M008470200

2 T. Happe, unpublished results.


    ABBREVIATIONS

The abbreviations used are: RACE, rapid amplification of cDNA ends; PCR, polymerase chain reaction; bp, base pair(s); PAGE, polyacrylamide gel electrophoresis; PSI, photosystem I.


    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES


1. Wu, L.-F., and Mandrand, M. A. (1993) FEMS Microbiol. Rev. 104, 243-270[CrossRef]
2. Albracht, S. P. J. (1994) Biochim. Biophys. Acta 1188, 167-204[Medline] [Order article via Infotrieve]
3. Przybyla, A. E., Robbins, J., Menon, N., and Peck, H. D., Jr. (1992) FEMS Microbiol. Rev. 88, 109-136[CrossRef]
4. Volbeda, A., Charon, M. H., Piras, C., Hatchikian, E. C., Frey, M., and Fontecilla-Camps, J. C. (1995) Nature 373, 580-587[CrossRef][Medline] [Order article via Infotrieve]
5. Adams, M. W. W. (1990) Biochim. Biophys. Acta 1020, 115-145[Medline] [Order article via Infotrieve]
6. Nicolet, Y., Lemon, B. J., Fontecilla-Camps, J. C., and Peters, J. W. (2000) Trends Biochem. Sci. 25, 138-142[CrossRef][Medline] [Order article via Infotrieve]
7. Zirngibl, C., van Dongen, W., Schworer, B., von Bunau, R., Richter, M., Klein, A., and Thauer, R. K. (1992) Eur. J. Biochem. 208, 511-520[Abstract]
8. Thauer, R. K., Klein, G., and Hartmann, C. (1996) Chem. Rev. 96, 3031-3036[CrossRef][Medline] [Order article via Infotrieve]
9. Meyer, J., and Gagnon, J. (1991) Biochemistry 30, 9697-9704[Medline] [Order article via Infotrieve]
10. Bui, E. T. N., and Johnson, P. J. (1996) Mol. Biochem. Parasitol. 76, 305-310[CrossRef][Medline] [Order article via Infotrieve]
11. Akhmanova, A., Voncken, F. G. J., van Alen, T., van Hoek, A., Boxma, B., Vogels, G. D., Veenhuis, M., and Hackstein, J. H. P. (1998) Nature 396, 527-528[CrossRef][Medline] [Order article via Infotrieve]
12. Verhagen, M.-F., O'Rourke, T., and Adams, M. W. W. (1999) Biochim. Biophys. Acta 1412, 212-229[Medline] [Order article via Infotrieve]
13. Atta, M., and Meyer, J. (2000) Biochim. Biophys. Acta 1476, 368-371[Medline] [Order article via Infotrieve]
14. Peters, J. W. (1999) Curr. Opin. Struct. Biol. 9, 670-676[CrossRef][Medline] [Order article via Infotrieve]
15. Adams, M. W. W., Mortenson, L. E., and Chen, J. S. (1981) Biochim. Biophys. Acta 594, 105-176
16. Peters, J. W., Lanzilotta, W. N., Lemon, B. J., and Seefeldt, L. C. (1998) Science 282, 1853-1858[Abstract/Free Full Text]
17. Nicolet, Y., Piras, C., Legrand, P., Hatchikian, E. C., and Fontecilla-Camps, J. C. (1999) Structure 7, 13-23[CrossRef][Medline] [Order article via Infotrieve]
18. Adams, M. W. W., and Stiefel, E. I. (2000) Curr. Opin. Chem. Biol. 4, 214-220[CrossRef][Medline] [Order article via Infotrieve]
19. Cammack, R. (1999) Nature 397, 214-215[CrossRef][Medline] [Order article via Infotrieve]
20. Gaffron, H. (1939) Nature 143, 204-205
21. Gaffron, H. (1940) Am. J. Bot. 27, 273-283
22. Gaffron, H., and Rubin, J. (1942) J. Gen. Physiol. 26, 219-240[Free Full Text]
23. Bishop, N. I. (1966) Annu. Rev. Plant. Physiol. 17, 185-208
24. Senger, H, and Bishop, N. I. (1979) Planta 145, 53-62
25. Randt, C., and Senger, H. (1985) Photochem. Photobiol. 42, 553-557
26. Ben-Amotz, A., and Gibbs, M. (1975) Biochem. Biophys. Res. Commun. 64, 335-359
27. Boichenko, V. A., and Hoffmann, P. (1994) Photosynthetica 30, 527-552
28. Bamberger, E. S., King, D., Erbes, D. L., and Gibbs, M. (1982) Plant Physiol. 69, 1268-1273
29. Greenbaum, E., and Lee, J. W. (1998) Biohydrogen 31, 235-240
30. Happe, T., and Naber, J. D. (1993) Eur. J. Biochem. 214, 475-481[Abstract]
31. Happe, T., Mosler, B., and Naber, J. D. (1994) Eur. J. Biochem. 222, 769-774[Abstract]
32. Schnackenberg, J., Schulz, R., and Senger, H. (1993) FEBS Lett. 327, 21-24[CrossRef][Medline] [Order article via Infotrieve]
33. Zinn, T., Schnackenberg, J., Haak, D., Romer, S., Schulz, R., and Senger, H. (1994) Z. Naturforsch. 49, 33-38
34. German, D. S., and Levine, R. P. (1965) Proc. Natl. Acad. Sci. U. S. A. 54, 1665-1669[Medline] [Order article via Infotrieve]
35. Schmitter, J. M., Jacquot, J.-P., de Lamotte-Guery, F., Beauvallet, C., Dutka, S., Gadal, P., and Decottignies, P. (1988) Eur. J. Biochem. 172, 405-412[Abstract]
36. Chenchik, A., Moqadam, F., and Siebert, P. (1996) in A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis (Krieg, P. A, ed) , pp. 273-321, Wiley-Liss, Inc., New York
37. Gong, B., and Ge, R. (2000) BioTechniques 28, 846-852[Medline] [Order article via Infotrieve]
38. Siebert, P. D., Chenchik, A., Kelogg, D. E., Lukyanov, K. A., and Lukyanov, S. A. (1995) Nucleic Acids Res. 23, 1087-1088[Medline] [Order article via Infotrieve]
39. Sanger, F., Nicklen, S., and Coulson, A. (1977) Proc. Natl. Acad. Sci. U. S. A. 74, 5463-5467[Abstract]
40. Johanningmeier, U., and Howell, S. H. (1984) J. Biol. Chem. 259, 13541-13549[Abstract/Free Full Text]
41. Sambrook, J., Tritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual , 2nd Ed. , Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
42. Quinn, J. M., and Merchant, S. (1998) Methods Enzymol. 297, 263-279[Medline] [Order article via Infotrieve]
43. Thompson, J. D., Higgins, D. G., and Gibson, T. J. (1994) Nucleic Acids Res. 22, 4673-4680[Abstract]
44. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D. J. (1985) Nucleic Acids Res. 25, 3389-3402[Abstract/Free Full Text]
45. Silflow, C. D., Chisholm, R. L., Conner, T. W., and Ranum, L. P. (1985) Mol. Cell. Biol. 5, 2389-2398[Medline] [Order article via Infotrieve]
46. Silflow, C. D. (1998) in The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas (Rochaix, J. D. , Goldschmidt-Clermont, M. , and Merchant, S., eds) , pp. 25-40, Kluwer Academic Publishers, Dordrecht, The Netherlands
47. Franzen, L. G., Rochaix, J. D., and von Heijne, G. (1990) FEBS Lett. 260, 165-168[CrossRef][Medline] [Order article via Infotrieve]
48. Keegstra, K. (1989) Cell 56, 247-253[Medline] [Order article via Infotrieve]
49. Deleted in proof
50. Voordouw, G., Hagen, W. R., Kruse-Wolters, K. M., van Berkel-Arts, A., and Veeger, C. (1987) Eur. J. Biochem. 162, 31-36[Abstract]
51. Stokkermans, J., van Dongen, W., Kaan, A., van den Berg, W., and Veeger, C. (1989) FEMS Microbiol. Lett. 49, 217-222[Medline] [Order article via Infotrieve]
52. Francis, K. (1989) Photosynthetica 23, 43-48
53. Kaji, M., Taniguchi, Y., Matsushita, O., Katayama, S., Miyata, S., Morita, S., and Okabe, A. (1999) FEMS Microbiol. Lett. 181, 329-336[CrossRef][Medline] [Order article via Infotrieve]
54. Gorwa, M.-F., Croux, C., and Soucaille, P. (1996) J. Bacteriol. 178, 2668-2675[Abstract]
55. Trebst, A. (1980) Methods Enzymol. 69, 675-715
56. Moulis, J.-M., and Davasse, V. (1995) Biochemistry 34, 16781-16788[Medline] [Order article via Infotrieve]
57. Guex, N., Diemand, A., and Peitsch, M. C. (1999) Trends Biochem. Sci. 24, 364-367[CrossRef][Medline] [Order article via Infotrieve]
58. Bes, M. T., Parisini, E., Inda, L. A., Sraiva, L. M., Peleato, M. L., and Sheldrick, G. M. (1999) Structure 7, 1201-1213[CrossRef][Medline] [Order article via Infotrieve]
59. Guex, N., and Peitsch, M. C. (1997) Electrophoresis 18, 2714-2723[Medline] [Order article via Infotrieve]


Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.