Page 8423, Fig. 9: The wrong figure was
published although the legend is correct. The correct figure is shown
below.
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Fig. 9.
Intracellular distribution of nascent
apoB-containing lipoproteins in microsomal lumen of control and
fructose-fed hepatocytes. Cultured primary hamster hepatocytes
were pulsed for 45 min with [35S]methionine, and the
radioactivity was chased for 0 or 1 h. Labeled cells were then
subjected to homogenization and fractionation of microsomes. Luminal
lipoproteins were extracted from microsomes by carbonate treatment and
were separated from the membrane fraction by centrifugation followed by
fractionation on a sucrose-gradient. After centrifugation,
gradient fractions were collected and immunoprecipitated with an
anti-hamster apoB antibody. Immunoprecipitates were analyzed by
SDS-PAGE and fluorography, and apoB radioactivity was quantitated by
cutting and scintillation counting of the apoB-100 band. A,
luminal lipoproteins in control hepatocytes at 0 and 1 h chase;
B, luminal lipoproteins in fructose-fed hepatocytes at 0 and
1 h chase.
Data Interpretation: The interpretation of the data in Fig. 9 does not significantly change with this correction. However, an important observation also reported previously should be emphasized further. As observed in the corrected Fig. 9, microsomes from control cells had a significant amount of dense apoB-containing lipoproteins (HDL density), at both 0 and 1 h, which were absent in hepatocytes from fructose-fed hamsters. The absence of HDL-dense apoB particles suggests the stimulated state of VLDL assembly and secretion in hepatocytes of fructose-fed hamsters.
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