From the Department of Chemistry and Biochemistry, Concordia University, Montreal, Quebec H3G 1M8, Canada
Received for publication, January 24, 2001, and in revised form, March 19, 2001
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ABSTRACT |
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Protein-based radicals generated in the reaction
of ferricytochrome c (cyt c) with
H2O2 were investigated by electrospray mass
spectrometry (ESI-MS) using 3,5-dibromo-4-nitrosobenzenesulfonate (DBNBS). Up to four DBNBS-cyt c adducts were observed in
the mass spectra. However, by varying the reaction conditions (0-5
molar equivalents of H2O2 and substituting cyt
c with its cyanide adduct which is resistant to
peroxidation), noncovalent DBNBS adduct formation was inferred.
Nonetheless, optical difference spectra revealed the presence of a
small fraction of covalently trapped DBNBS. To probe the nature of the
noncovalent DBNBS adducts, the less basic proteins, metmyoglobin (Mb)
and Reactive oxygen species, such as H2O2 and
superoxide, are generated by all aerobic cells as by-products of a
number of metabolic reactions and in response to various stimuli.
Oxidative damage can occur when H2O2 reacts
with heme proteins, such as ferricytochrome c (cyt
c),1 to form
highly reactive oxyferryl-heme and transient protein-based radical
species (X·) that are linked to the initiation of lipid
peroxidation (1, 2). Detection of X· in biological systems is
often difficult because they are short-lived and highly reactive. Spin
traps, which are diamagnetic compounds containing a functional group
that reacts with X· to form a more stable paramagnetic adduct
(XST·), are frequently used in electron paramagnetic resonance
(EPR) investigations (3). Although EPR signals can provide information about a radical center and its environment, the specific
sites of radical formation in biomolecules are not identified.
Coupling of high performance liquid chromatography (HPLC) and mass
spectrometry (MS) has been used to identify spin adducts of various
small molecules (4-6). Our research group has extended the use of
LC/MS of spin adducts to proteins to overcome the inherent limitations
of EPR. We have found that conversion of the spin adduct XST· to
a stable diamagnetic mass adduct (XMA) via ascorbate reduction permits
the assignment of XMA to a specific amino acid residue when
spin trapping and peptide mass mapping by on-line LC/MS (ST/LC/MS) are
coupled (7-10). In addition to the increased specificity offered by
ST/LC/MS, it possesses enhanced sensitivity over EPR, since considerably smaller quantities (picomole versus nanomole)
of sample can be analyzed (11).
3,5-Dibromo-4-nitrosobenzenesulfonate (DBNBS), sometimes referred to as
Perkin's trap, was developed to trap carbon-centered radicals. It is
stable to temperature and light, and the introduction of the sulfonate
group onto the benzene ring has helped overcome problems encountered in
the use of lipophilic nitroso spin traps such as
2-methyl-2-nitrosopropane (MNP) (12). However, it is known that DBNBS
adducts can be formed through several nonradical reactions. For
example, prolonged incubation of DBNBS with unsaturated fatty acids
(13) or with free tryptophan (14) causes chemical modifications through
nonradical reactions such as the ene reaction between the nitroso group
in DBNBS and the double bond in tryptophan.
Barr and co-workers (11, 15) recently reported the trapping of a
protein-based tyrosyl radical by DBNBS in the reaction of cyt
c with H2O2 using EPR. In addition,
peaks corresponding to (DBNBS)n-cyt c adducts, with
n = 1-4, were observed by MALDI-MS, suggesting that as
many as four protein-based X· species were trapped by DBNBS
during the reaction of cyt c with 5 molar equivalents of
H2O2. However, the sites of DBNBS-cyt
c adduct formation were not identified. The radicals formed
in the reaction of cyt c with H2O2
are of interest, since they could cause mitochondrial membrane damage
and play a role in the apoptotic process (16). Using ST/LC/MS, we
reexamined the products of the cyt
c/H2O2/DBNBS reaction to determine
the extent of protein radical formation. Surprisingly, we detected
DBNBS-cyt c mass adducts in the absence of
H2O2, and in the cyanide-ligated protein, even
though cyanide binding inhibits heme-mediated peroxidation (11,
17).
Cyt c, a highly basic protein (pI ~10), unfolds at low pH,
resulting in increased absorption and blue-shifting of the Soret maximum from 408 at pH 7.0 to 394 nm in the acid-denatured protein at
pH 2.0 (18). The addition of anions converts the unfolded state of cyt
c to a conformation resembling a molten globule by reducing
the electrostatic repulsion of positive charges on the protein surface
via Debye-Hückel screening and ion pairing (18, 19). We provide
data here that support the formation of noncovalent adducts under the
MS conditions (pH 2.0); in fact, DBNBS was found to stabilize a molten
globule state of acid-unfolded cyt c at pH 2.0 in a manner
similar to that reported for 8-anilino-1-naphthalenesulfonate (ANS)
(19). Our results reveal that caution must be used in interpretation of
the mass spectra of DBNBS-protein adducts, and the different
limitations in EPR and MS approaches to the analysis of spin-trapped
species are also highlighted.
Materials--
Horse (Type VI), cow and tuna (Type XI) heart
cytochromes c, horse heart metmyoglobin (Mb),
Methods--
Reactions were carried out by mixing 500 µM protein with 10 mM DBNBS and 0, 1, or 5 molar equivalents of H2O2 in 50 mM
ammonium acetate solution (pH 7.5) containing 200 µM
DTPA. The reactions were terminated after 10 min by injecting the
reactants onto a Vydac C18 column (4.6 × 300 mm) and
separating the protein from salts and low molecular weight reactants by
HPLC (HP1090, Hewlett-Packard) using a 10-55% acetonitrile (ACN)
gradient in 0.05% trifluoroacetic acid at 1 ml/min over 20 min. The
protein peak was collected, lyophilized, suspended in 1:1
methanol/water with 0.5% acetic acid, and infused at a flow rate of 5 µl/min using a syringe pump (Harvard Apparatus) directly into the
electrospray ionization (ESI) source of a Finnigan SSQ7000 mass
spectrometer (ThermoFinnigan) for molecular weight determination. The
protein was further digested with 1:50 (w/w) trypsin at 50 °C for
4 h. The digests were separated for peptide mass mapping on a
Vydac microbore C18 column (1 × 300 mm) using a
10-55% ACN gradient in 0.05% trifluoroacetic acid at 40 µl/min
over 100 min. The spray voltage was set at 4.5 kV, and the capillary
temperature was maintained at 210 °C, while the sheath and auxiliary
gas pressures were 40 and 15 p.s.i., respectively. Full-scan
acquisition was performed in profile mode using scan rates of 320-380
atomic mass units/s.
To probe DBNBS interaction with acid-unfolded cyt c, the
protein was incubated for 30 min with increasing amounts of DBNBS in
0.05% trifluoroacetic acid (pH 2.0) before carrying out optical or MS
measurements. For molecular weight determination, samples were infused
into the ESI source of the mass spectrometer by flow injection at 50 µl/min in 75% ACN containing 0.05% trifluoroacetic acid. Optical
spectra were recorded on a Beckman DU 650 spectrophotometer between 200 and 600 nm, using a scan rate of 2400 nm/min. Difference spectra were
generated using Origin 3.0 software (MicroCal).
Analysis of DBNBS Mass Adduct Formation with Ferricytochrome c in
the Presence and Absence of H2O2--
The
deconvolved ESI mass spectrum (Fig.
1a) of horse cyt c
revealed that (DBNBS)n-cyt c adducts
(n = 1-4) were formed in the reaction of cyt
c with 5-fold molar excess of H2O2,
as observed previously by MALDI-MS (11, 15). A similar pattern of
(DBNBS)n-cyt c adduct formation was observed when cyt c was reacted with only 1 molar equivalent of
H2O2 (Fig. 1b) despite the fact that
H2O2, a two-electron oxidant, can remove a
maximum of two electrons to generate two radical sites on cyt c. The reaction of horse cyt c with 1-5 molar
equivalents of H2O2 was repeated several times
in the presence of DBNBS, and multiple DBNBS adducts were consistently
observed. Moreover, in the absence of H2O2, up
to two DBNBS adducts were detected not only with horse cyt
c, but also with bovine and tuna cyts c (Fig. 1,
c-e). These data suggest that DBNBS interacts with cyt
c via a non-spin-trapping mechanism.
The cyanide ion serves as a high affinity ligand for the ferric state
of cyt c by displacing the axial Met80 ligand
(17, 20). Hence, cyanocyt c formation, which inhibits heme-catalyzed reactions and prevents cyt c heme degradation
by organic hydroperoxides (11, 17), was used to probe direct (i.e. non-heme mediated) peroxidation of the polypeptide.
Fig. 1f reveals that the addition of 3 mM KCN to
the horse cyt c/H2O2/DBNBS reaction
results in the same mass spectrum as that observed in the absence of
H2O2 (Fig. 1, f versus
c). Therefore, the (DBNBS)n-cyt c
adducts from the cyanocyt
c/H2O2/DBNBS reaction are unlikely due to peroxidation, supporting the absence of non-heme-mediated oxidation of cyt c residues by H2O2.
Nonetheless, the mass spectra reveal that the (DBNBS)n-cyt
c adduct intensities increase in the presence of
H2O2 (Fig. 1, a and
b versus c); hence, spin trapping of
X· by DBNBS is also likely occurring, consistent with the EPR
data that indicated trapping of a tyrosyl radical in cyt c
(11).
To further probe the nature of the DBNBS adducts detected by MS,
optical spectra of the reaction products obtained for horse cyt
c in the absence and presence of
H2O2 were recorded (Fig. 2). The difference spectrum (Fig. 2,
trace 3) of HPLC-purified products from the cyt
c/H2O2/DBNBS (1:5:20) reaction minus
those from the cyt c/H2O2 (1:5)
reaction reveals loss of DBNBS absorption seen at 288 nm (Fig. 2,
trace 2) and growth of a new absorption band at 300 nm. This
is consistent with increased conjugation of the chromophore, where the
nitroso group of DBNBS traps a radical on the aromatic ring of a
tyrosine residue (11). The possibility that the 300 nm absorption was
due to H2O2-induced oxidation of DBNBS was also
considered, but incubation of DBNBS with 5 molar equivalents of
H2O2 gave rise to a DBNBS species with an
absorption spectrum (Fig. 2, trace 1) essentially identical
to that of untreated DBNBS (Fig. 2, trace 2). Moreover, the
cyt c/DBNBS (1:10) minus cyt c difference
spectrum (data not shown) resembles that of DBNBS alone (Fig. 2,
trace 2). As an additional control, the cyt
c/H2O2 (1:5) minus cyt c
difference spectrum (Fig. 2, trace 4) was generated. A
relatively flat base line between 230 and 370 nm with negligible UV
absorption was observed, suggesting minimal oxidation of aromatic residues by H2O2. Thus, the new band at 300 nm
in the cyt c/H2O2/DBNBS minus cyt
c/H2O2 difference spectrum (Fig. 2,
trace 3) is not an artifact due to the subtraction
procedure, but can be assigned to a protein-based DBNBS spin adduct.
Unfortunately, tryptic digestion of the products of the cyt
c/H2O2/DBNBS reaction yielded only native peptides. It has been observed that ascorbate reduction of
MNP-Mb spin adducts yields stable XMAs that can be identified by
ST/LC/MS (9, 10). However, addition of 5 mM ascorbate to
the cyt c/H2O2/DBNBS reaction did
not convert XST· to XMA, since the mass spectrum (data not
shown) was the same as that in Fig. 1a. This is likely due
to either low trapping efficiency of DBNBS toward cyt c
radicals or instability of the covalently trapped DBNBS-cyt
c adducts under the digestion conditions. The radical is
bonded directly to the nitroxide in the XST· formed with DBNBS,
which renders reverse or cleavage reactions of the spin-trapped species
favorable (3). Interestingly, Kim and co-workers (21) observed by EPR
that the decay of 5,5-dimethylpyrroline-N-oxide (DMPO) adducts of Mb
and hemoglobin were accelerated by denaturation (urea or guanidine HCl)
and proteolysis of the protein moiety. The data in Fig. 2 demonstrate,
nonetheless, that DBNBS-cyt c spin adducts formed in the
presence of H2O2 can be distinguished from
those formed in the absence of H2O2 by optical
difference spectroscopy.
Many reactions other than spin trapping have been reported for DBNBS.
For example, the formation of a nitroxyl free radical was detected by
EPR after a 60-min incubation of DBNBS with free tryptophan (14).
Chemical modification after 24-h incubation of low density lipoprotein
by DBNBS was detected by agarose gel electrophoretic mobility (13). The
DBNBS labeling was assigned to the ene reaction between the nitroso
group and a double bond, which results in an allylic hydroxylamine
(22). However, both reported studies indicated that labeling required
prolonged (
The possibility of "inverted spin trapping" was also considered,
which could occur if the cyt
c/H2O2/DBNBS reaction yielded an
oxidized form of the spin trap (DBNBS·+). Inverted
spin trapping of DBNBS·+ by an amino acid residue
could lead to a DBNBS-cyt c adduct identical to that formed
by "normal" spin trapping of X· by DBNBS (reviewed in Ref.
23). Horseradish peroxidase/H2O2 (E° FeIV = O/FeIII = 0.94 V)
catalyzed the oxidation of DBNBS over a 24-h incubation, as seen by EPR
(24). However, DBNBS·+ generation was not observed
by the peroxidase activity (E° FeIV = O/FeIII ~ 1 V) of Mb/H2O2 or cyt
c oxidase/H2O2 (25, 26), the Fenton reaction, FeII/H2O2
(E° FeIII/FeII = 0.77 V) (27), nor
in the oxidation of selenite by CeIV (E°
CeIV/CeIII = 1.44 V) (28). Since MNP and DBNBS
contain identical redox-active nitroso functional groups, it can be
assumed that the E° DBNBS·+/DBNBS is
comparable with E° MNP·+/MNP (2.06 V)
(23), which is beyond the reach of the high oxidation states of heme
proteins under non-forcing conditions. Hence, "inverted spin
trapping" is ruled out in the cyt
c/H2O2/DBNBS reaction, since (i) the
reaction time was only 10 min and (ii) the FeIV = O center
is rapidly converted to FeIII by endogenous electron
transfer to heme (data not shown). Also, the reduction potential of the
FeIII/FeII couple in horse cyt c
(E° = 0.25 V) is clearly insufficient to oxidize the
nitroso group.
DBNBS could also form sulfonamides with the Analysis of DBNBS Mass Adduct Formation with Metmyoglobin and
In Fig. 3a, the deconvolved mass spectrum of the
Mb/H2O2/DBNBS (1:5:20) reaction products shows
the formation of (DBNBS)n-Mb adducts with n = 1 and 2. DBNBS ene addition to Mb and/or ion pair formation can be
ruled out, since only native globin is detected in the deconvolved mass
spectrum in the absence of H2O2 (Fig. 3b). Inverted spin trapping was not reported in the
Mb/H2O2/DBNBS reaction (26), since the
oxyferryl heme of Mb (E° FeIV = O/FeIII ~ 1 V) cannot oxidize DBNBS. When
H2O2 was present at 1 molar equivalent (Fig.
3c), the relative intensity of the (DBNBS)2-Mb peak was negligible and that of the (DBNBS)-Mb peak decreased by 40%
compared with the 1:1 adduct following oxidation with 5 molar
equivalents of H2O2 (Fig. 3a).
Likewise, a less intense (DMPO)-Mb peak was observed by ESI-MS upon
decreasing the amount of H2O2 from 3 to 1 molar
equivalents in the Mb/H2O2/DMPO reaction (32).
It was reported that formation of oxyferryl Mb requires >1 molar
equivalent of H2O2, since
H2O2 is consumed in side reactions at the
porphyrin or other locations on the globin (9, 34). Nevertheless, the
efficiency of (XST·)n-Mb adduct formation depends on the
number of oxidizing equivalents present in the
Mb/H2O2/ST reaction for both DMPO and DBNBS,
in contrast to the cyt c/H2O2/DBNBS
reaction (Fig. 1, a versus b).
DBNBS adduct formation with DBNBS Adduct Formation with Horse Cyt c in the Absence of
H2O2 at pH
2.0--
Anion-dependent stabilization of compact
structures that resemble the molten globule state have been observed by
monitoring the Soret band upon incubation of cyt c with ANS
or with various strong acids and their neutral salts (18, 19). The
molten globule state of cyt c, which forms at high salt (500 mM NaCl) and low pH (pH 2.0), is characterized by a red
shift from 394 nm (the Soret maximum of acid-denatured cyt
c) to 400 nm, which is accompanied by band broadening (18).
To elucidate the mechanism by which the (DBNBS)n-cyt
c mass adducts (Fig. 1) are formed, acid-unfolded horse cyt
c was titrated with DBNBS at pH 2.0, and changes in the
Soret and mass spectra were monitored. The Soret maximum red-shifted
from 394 to 402 nm following 30-min incubation of cyt c with
25 molar excess DBNBS, consistent with molten globule formation (Fig.
5a). Similar effects were
observed with ANS (19), which promotes the refolding of cyt
c at low pH by Debye-Hückel screening and ion pair
formation (18). The mass spectra in Fig. 1, a and b
versus c, reveal enhanced binding of DBNBS to the
H2O2-oxidzied cyt c, which is also
seen in the greater red shifting of the Soret bands in Fig. 5,
b versus a. Of note, the absorbencies
of the acid denatured oxidized cyt c (
DBNBS titration of horse cyt c was also monitored by MS
(Fig. 6). The (DBNBS)n-cyt
c (n = 1-4) peaks in the mass spectra of
samples with
Precipitation of cyt c was observed upon addition of a large
excess of DBNBS at pH 7.5, which required limiting the amount of DBNBS
added to 500 µM cyt c to 10 mM.
Ion pair formation between the sulfonate group of DBNBS and basic
residues of cyt c would reduce the solubility of the
protein. Interestingly, cyt c is known to possess anionic
binding sites; specifically, there are two phosphate binding sites, one
near Lys87 with a dissociation constant
(Kd) of 200 µM and another close to
Lys25-His26-Lys27 with a
Kd of >2 mM (36). However, the
DBNBS-cyt c adducts must possess Kd
values in the low µM range, since phosphate-cyt
c adducts are not observed by ESI-MS following
reversed-phase HPLC purification, whereas DBNBS-cyt
c adducts are seen in Fig. 1, c-e. In fact, a
Kd of ~36 µM was estimated
from a double-reciprocal plot ( Conclusions--
DBNBS complexes with cyt c via strong
electrostatic interactions at pH 2.0, thereby complicating the analysis
of spin trapping by ST/LC/MS. However, noncovalent DBNBS adduct
formation clearly shows specificity for cyt c (Fig. 1,
c-e versus Figs. 3b and
4b), indicating that its formation cannot simply be
correlated with the total number of lysine residues, since horse cyt
c possesses 19, while horse Mb and cow -lactalbumin, were substituted for cyt c in the cyt
c/H2O2/DBNBS reaction. A maximum of
two DBNBS adducts were observed in the mass spectra of the products of
the Mb/H2O2/DBNBS reactions, whereas no adducts
were detected following
-lactalbumin/H2O2/DBNBS incubation, which is
consistent with adduct formation via spin trapping only. Titration with
DBNBS at pH 2.0 yielded noncovalent DBNBS-cyt c adducts and induced folding of acid-denatured cyt c, as monitored by
ESI-MS and optical spectroscopy, respectively. Thus, the noncovalent DBNBS-cyt c mass adducts observed are assigned to ion pair
formation occurring between the negatively charged sulfonate group on
DBNBS and positively charged surface residues on cyt c. The
results reveal the pitfalls inherent in using mass spectral data with negatively charged spin traps such as DBNBS to identify sites of
radical formation on basic proteins such as cyt c.
INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS AND DISCUSSION
REFERENCES
EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS AND DISCUSSION
REFERENCES
-lactalbumin from bovine milk, trifluoroacetic acid, and
DBNBS were purchased from Sigma and used without further purification.
H2O2 was purchased from Fisher, while potassium
cyanide (KCN) was obtained from BDH Chemicals. Sequencing grade trypsin
(Roche Molecular Biochemicals) was used for digestion, and ammonium
acetate (JT Baker Chemical Co.) Solutions containing
diethylenetriamine-N,N,N',N",N"-pentaacetic
acid (DTPA, ICN) were prepared using 18-M
-cm water obtained from a
Barnstead Nanopure system.
RESULTS AND DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS AND DISCUSSION
REFERENCES
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Fig. 1.
Deconvolved ESI mass spectra of the products
of a 10-min reaction of 500 µM cyt
c with H2O2 in the presence of
10 mM DBNBS. a, horse cyt c with
2.5 mM H2O2; b, horse
cyt c with 500 µM
H2O2; c, horse cyt c
without H2O2; d, tuna heart cyt
c without H2O2; e, cow
heart cyt c without H2O2;
f, horse cyt c with 2.5 mM
H2O2 and 3 mM KCN (to form cyanocyt
c). Reactions were carried out in 50 mM ammonium
acetate (pH 7.5) containing 200 µM DTPA. The
HPLC-purified reaction products (see "Experimental Procedures")
were collected, lyophilized, and suspended in 1:1 methanol/water with
0.5% acetic acid. Samples (~1 mg/ml) were directly infused into the
ESI source of the mass spectrometer at 5 µl/min for molecular weight
determination.
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Fig. 2.
Absorption spectra of (trace
1) 132 µM DBNBS
following a 10-min incubation with 660 µM H2O2 and
(trace 2) untreated 111 µM DBNBS. Difference optical
spectra of the HPLC-purified DBNBS-cyt c adduct (~11
µM cyt c) minus the products from the 10-min
reaction of 11 µM horse cyt c with 55 µM H2O2 (trace 3) and
11 µM horse cyt c following a 10-min
incubation with 55 µM H2O2 minus
11 µM horse cyt c (trace 4) are
shown. All solutions were prepared in 50 mM ammonium
acetate (pH 7.5) containing 200 µM DTPA. Spectra were
recorded at a scan rate of 2400 nm/min, and difference spectra were
generated using Origin 3.0 software.
1 h) exposure to DBNBS. We limited cyt c
exposure to DBNBS to 10 min and immediately separated the protein from
the low molecular weight reactants by HPLC.
-amino groups of
lysines, which account for ~20% of the residues in cyt c.
However, the deconvolved mass spectra (Fig. 1, a-f) reveal
m increments of 360 ± 2 Da for the cyt c
adducts, corresponding to ammoniated DBNBS adducts, whereas sulfonamide
formation would give rise to
m increments of 326 Da.
Furthermore, peptide mass mapping of the products of the cyt
c/H2O2/DBNBS reaction yielded
exclusively native horse cyt c peptides (data not shown),
indicating that no stable derivatives such as sulfonamides were formed.
This agrees with the work of Kalyanaraman and co-workers (13) who
reported that DBNBS-modified low density lipoprotein was formed by a
lysine-independent process.
-Lactalbumin in the Presence and Absence of
H2O2--
To establish whether or not DBNBS
adduct formation with proteins exhibits specificity, Mb and
-lactalbumin were selected for further MS investigations. Mb, a
heme-containing protein with a pI of 7.0, is not reported to have a
high affinity for anionic compounds (29). Spin trapping of X·
generated in the Mb/H2O2 reaction has been
demonstrated by both EPR and ST/LC/MS using DBNBS and other spin traps,
and X· has been assigned primarily to Tyr103 (10,
30-33). The difference spectrum (data not shown) of HPLC-purified products from the Mb/H2O2/DBNBS (1:5:20)
reaction minus those from the Mb/H2O2 (1:5) is
similar to that seen with cyt c (Fig. 2, trace
3), which is indicative of DBNBS-tyrosine spin adduct formation in
both proteins. An estimate of
302 for the DBNBS-tyrosine spin adduct (42 mM
1
cm
1) was obtained by assuming a trapping
efficiency of 70% for the tyrosyl radical in Mb, based on the relative
intensities of the peaks in the mass spectrum of the
Mb/H2O2/DBNBS (1:5:20) reaction products (Fig.
3a). Using this estimated
302, the yield of DBNBS-cyt c spin adduct
formation in the cyt c/H2O2/DBNBS
(1:5:20) reaction is ~20%, compared with ~10% estimated from EPR
measurements (11). Yields of 10-20% are sufficient to identify
modified peptides by mass mapping, but tryptic digests of DBNBS-labeled
cyt c contained exclusively native peptides, indicating that
DBNBS labeling is not stable to peptide mass mapping, as discussed
above.
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Fig. 3.
Deconvolved ESI mass spectra of the products
of a 10-min reaction of 500 µM
horse heart metmyoglobin with H2O2 in the
presence of 10 mM DBNBS: with 2.5 mM
H2O2
(a), without
H2O2
(b), and with 500 µM H2O2
(c). Experimental
conditions are given in the legend to Fig. 1.
-lactalbumin was also investigated here,
since it is an acidic protein with a pI ~4 and contains basic
residues evenly dispersed over its surface. In fact, due to its high
negative charge under the spin trapping conditions used (pH 7.5),
-lactalbumin should repel the negatively charged DBNBS. Similarly,
Matulis and co-workers (35) observed by fluorescence spectroscopy that
very little ANS bound to bovine serum albumin (pI 5.8) at pH > 11.
-Lactalbumin also lacks the heme prosthetic group found in Mb
and cyt c; thus there should be no reaction with
H2O2 to generate X· unless
H2O2 directly oxidizes the polypeptide. As
expected, the deconvolved mass spectra of
-lactalbumin both in the
presence (Fig. 4a) and absence
(Fig. 4b) of H2O2 revealed no DBNBS
adduct formation, since only native
-lactalbumin was detected. This rules out direct peroxidation of the polypeptide, consistent with the
results for cyanocyt c (Fig. 2f), and it also
rules out ion pair formation.
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Fig. 4.
Deconvolved ESI mass spectra of the products
of a 10-min reaction of 500 µM
cow -lactalbumin with
H2O2 and 10 mM DBNBS: with 2.5 mM H2O2
(a) and without
H2O2
(b). Experimental
conditions are given in the legend to Fig. 1.
394 = 140 mM
1
cm
1) and its DBNBS-stabilized molten globule
(
402 = 94 mM
1
cm
1) are less than those of the unoxidized
forms (
394 = 209 mM
1
cm
1;
394 = 120 mM
1
cm
1).
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Fig. 5.
Soret absorption spectra of 10 µM non-oxidized (a) and
oxidized (b) horse cyt c incubated
for 30 min in 0.05% trifluoroacetic acid (pH 2.0) in the presence of 0 (trace 1), 1 (trace 2), 5 (trace 3), 15 (trace 4), 20 (trace 5), and 25 (trace 6) molar
equivalents of DBNBS. Oxidized protein was prepared by treating
100 µM horse cyt c with 5 molar equivalents of
H2O2 for 5 min at pH 7.5. Spectra were recorded
at a scan rate of 2400 nm/min.
10-fold excess DBNBS recorded without prior HPLC
purification are more intense than the corresponding peaks in the
HPLC-purified sample (Fig. 6, c-f versus Fig.
1c). Fig. 6 also reveals that the abundance and
stoichiometry of the (DBNBS)n-cyt c adducts
increased as a function of DBNBS concentration, which is consistent
with noncovalent adduct formation. Ali and co-workers (19) observed
peaks corresponding to (ANS)n-cyt c adducts
(n = 1-7) at pH 2.0 by ESI-MS, but no adduct formation was observed with nile red, a neutral hydrophobic dye, revealing the
importance of electrostatic interactions.
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Fig. 6.
Deconvolved ESI mass spectra of 20 µM horse cyt c
incubated for 30 min in 0.05% trifluoroacetic acid (pH 2.0) in
the presence of 0 (a), 1 (b), 10 (c), 15 (d), 20 (e),
and 25 (f) molar equivalents of DBNBS. Samples in
75% ACN containing 0.05% trifluoroacetic acid were infused into the
ESI source of the mass spectrometer by flow injection at 50 µl/min.
Abs
1
versus [DBNBS]
1) of the data
in Fig. 5 for both oxidized and non-oxidized horse cyt c,
similar to the calculated Kd (3-50
µM) for the interaction of ANS to cationic polyamino
acids at pH 2.0 (35).
-lactalbumin have
19 and 12, respectively. Lysine residues are highly conserved in cyts
c and are clustered predominately around the exposed heme
edge, forming anionic binding sites (36). In Mb and
-lactalbumin,
the lysine residues are more or less distributed evenly over the entire
protein surface. Also, the fact that DBNBS and ANS exhibit comparable
efficiencies in inducing cyt c folding at low pH, despite
the significantly larger hydrophobic moiety of ANS, is consistent with
electrostatic interactions being the principal determinant of binding.
Ion pair formation between proteins and negatively charged probes such as DBNBS and ANS may be a common occurrence at the low pH values used
for ESI-MS analyses in positive ion mode. Therefore, it is essential to
carry out the appropriate controls before interpreting MS data
involving protein-probe adducts such as the (DBNBS)n-cyt c mass adducts observed in the present study. Finally, our
results reveal that compared with Tyr103 of Mb, the cyt
c radicals are not very reactive and/or accessible at pH
7.5.
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FOOTNOTES |
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* This work was supported by grants from the Natural Sciences and Engineering Research Council of Canada and the Fonds pour la Formation des Chercheurs et L'Aide à la Recherche (FCAR) of Quebec (to A. M. E.) and by scholarships from FCAR and the J. W. McConnell Memorial Graduate Fellowship Fund (Concordia University) (to A. F.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 514-848-3338;
Fax: 514-848-2868; E-mail: english@vax2.concordia.ca.
Published, JBC Papers in Press, March 21, 2001, DOI 10.1074/jbc.M100644200
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ABBREVIATIONS |
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The abbreviations used are: cyt c, ferricytochrome c; ACN, acetonitrile; ANS, 8-anilino-1-naphthalenesulfonate; DBNBS, 3,5-dibromo-4-nitrosobenzenesulfonate; DBNBS·+, oxidized 3,5-dibromo-4-nitrosobenzene sulfonate; DMPO, 5,5-dimethylpyrroline-N-oxide; DTPA, diethylenetriamine-N,N,N',N",N"-pentaacetic acid; ESI, electrospray ionization; EPR, electron paramagnetic resonance; HPLC, high-performance liquid chromatography; MALDI, matrix-assisted laser desorption ionization; Mb, metmyoglobin; MNP, 2-methyl-2-nitrosopropane; MNP·+, oxidized 2-methyl-2-nitrosopropane; MS, mass spectrometry; ST/LC/MS, spin trapping coupled to on-line peptide mass mapping; X·, protein-based radical; XMA, diamagnetic mass adduct of X·; XST·, spin adduct of X·.
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