Pages 3593 and 3594: Figs. 4 and 6 were
reversed although the legends are correct. The correct figures and
legends are shown below.
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Fig. 4.
The expression of myogenin in antisense
decorin clones is reverted by specific decorin re-expression and by the
addition of exogenous decorin. A: left panel, myogenin
expression was evaluated in wild type cells and antisense
decorin-transfected cells (WT and A6) and in
transfected cells infected with an adenovirus containing the
full-length sequence for human decorin (WTi and
A6i). Northern blot analysis for myogenin under growth
conditions was performed. 10 µg of total RNA isolated from myoblasts
was separated by electrophoresis, blotted onto nylon membranes, and
hybridized with a 32P-labeled myogenin probe (Myo).
The methylene blue-stained nylon membrane is shown in the lower
part of the panel, and the ribosomal RNAs are indicated. The
transcript size is indicated. Right panel, the extent of
decorin synthesized by the adenovirus-infected cells described in
A was evaluated by specific immunoprecipitation with
antibodies against human decorin as described under "Experimental
Procedures." B, A6 cells were incubated for 48 h with
decorin purified from bovine cartilage (left panel) or from
mouse skeletal muscle (right panel). Northern blot analysis
for the effect of bovine decorin is shown in the left panel
as described above. The reversion effect of skeletal muscle decorin on
myogenin expression was evaluated using A6 myoblasts transiently
cotransfected with pMyoLuc and pRL plasmids. The cells were incubated
for 24 h in growth medium, harvested, and dual luciferase activity was
determined as described under "Experimental Procedures."
C, A6 cells were incubated with skeletal muscle decorin
pretreated with chondroitinase ABC (Cabc) and assayed as
explained in the legend of B. Decorin concentrations are as
follows: D1 = 0.1 µM and D2 = 0.5 µM. The
results shown are the means from two different experiments performed in
duplicate.
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Fig. 6.
Concentration dependence of TGF- -mediated
inhibition of myogenin expression in control and antisense decorin
cells. Wild type (WT) and antisense decorin-transfected
cells (A6) were incubated for 30 h in differentiation medium
containing the indicated concentrations of TGF-
1 (A, left
panel) and FGF-2 (B, left panel). RNA was isolated from
the cells, and 10 µg of total RNA was analyzed by Northern blot with
a 32P-labeled myogenin (Myo) cDNA probe.
Right panels show the graphical representations of TGF-
1
(A)- and FGF-2 (B)-dependent inhibition of
myogenin expression in antisense decorin cells (open
circles) and wild type cells (closed circles). Values
correspond to the means of three independent experiments. C
shows TGF-
1-dependent inhibition of myogenin expression in wild type
cells (closed circles) and wild type cells infected with
adenovirus containing the full-length sequence for human decorin
(open circles). Myogenin expression was evaluated as
described in the legend of Fig. 4B.
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