Inhibition of Cytochrome c Release in Fas-mediated Signaling Pathway in Transgenic Mice Induced to Express Hepatitis C Viral Proteins*

Keigo MachidaDagger §, Kyoko Tsukiyama-KoharaDagger , Eiji SeikeDagger , Shigenobu Toné||, Futoshi Shibasaki**, Masumi ShimizuDagger Dagger , Hidemi TakahashiDagger Dagger , Yukiko Hayashi§§, Nobuaki Funata§§, Choji Taya¶¶, Hiromichi Yonekawa¶¶, and Michinori KoharaDagger ||||

From the Dagger  Department of Microbiology and Cell Biology, The Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan, the § Department of Immunology, Graduate School of Medicine and Faculty of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan, the  Department of Internal Medicine, Self-Defense Forces Central Hospital, 1-2-24 Ikejiri, Setagaya-ku, Tokyo, 154-0001, Japan, the || Department of Biochemistry, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama, 7.1-0192, Japan, the ** Department of Molecular Cell Physiology, The Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan, the Dagger Dagger  Department of Microbiology and Immunology, Nippon Medical School, 1-1-5, Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan, the §§ Department of Pathology, Tokyo Metropolitan Komagome Hospital, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113-8677, Japan, and the ¶¶ Laboratory of Animal Science, The Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan

Received for publication, November 7, 2000, and in revised form, January 9, 2001



    ABSTRACT
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Persistent hepatitis C virus (HCV) infection often progresses to chronic hepatitis, cirrhosis, and hepatocellular carcinoma. Numerous viruses have been reported to escape from apoptotic mechanism to maintain persistent infection. In the present study, we characterized the effect of HCV proteins on the Fas signal using HCV transgenic mice, which expressed core, E1, E2, and NS2 proteins, regulated by the Cre/loxP switching system. The transgene expression of HCV transgenic mice caused resistance to Fas antibody stimulated lethality. Apoptotic cell death in the liver of HCV protein expressing mice was significantly reduced compared with nonexpressing mice. Histopathological analysis and DNA fragmentation analysis revealed that the HCV proteins suppressed Fas-mediated apoptotic cell death. To identify the target pathway of HCV proteins, we characterized caspase activity. The activation of caspase-9 and -3/7 but not caspase-8 was inhibited by HCV proteins. Cytochrome c release from mitochondria was inhibited in HCV protein expressing mice. These results indicated that the expression of HCV proteins may directly or indirectly inhibit Fas-mediated apoptosis and death in mice by repressing the release of cytochrome c from mitochondria, thereby suppressing caspase-9 and -3/7 activation. These results suggest that HCV may cause persistent infection, as a result of suppression of Fas-mediated cell death.



    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Hepatitis C virus (HCV)1 is a positive-strand RNA virus and major causative agent of post-transfusion-associated and sporadic nonA nonB hepatitis. Persistent HCV infection often progresses to chronic hepatitis, cirrhosis, and hepatocellular carcinoma. The mechanism involved in the development of persistent infection and the pathogenesis is still unclear.

A number of viruses have been reported to escape from the apoptotic mechanism to maintain persistent infection. The expression of HCV proteins is reported to influence apoptosis. HCV proteins have been variously found to suppress or activate anti-Fas antibody and/or tumor necrosis factor (TNF)-alpha mediated cell death (1-4). In addition, HCV reportedly activates nuclear factor kappa B (NF-kappa B) and represses Fas and TNF-alpha -mediated cell death (5-7). The effects of cell death by HCV proteins are not fully understood because several discrepancies have been observed in the activation and repression of Fas or TNF-alpha related cell death.

A transgenic mouse model using a stable expression system causes immunotolerance to transgene products. Therefore, an HCV protein switching expression system may be suitable for in vivo assay of HCV protein effects. Using the Cre/loxP system, we developed a transgenic mouse model with efficient conditional transgene activation of HCV cDNA (core, E1, E2, and NS2) (8). HCV proteins were mainly detected in the liver of conditionally expressing transgenic mice. These methods allowed us to investigate the importance of HCV proteins in apoptotic signaling pathways by the conditional expression of HCV proteins in vivo.

Fas-mediated cell death appears to induce hepatic injury (9). Fas-mediated liver injury is likely to play a critical role in some forms of immune-mediated hepatitis. It has become evident that activated T lymphocytes have the ability to kill Fas-bearing targets through membrane expression of Fas ligand (Fas-L) (10). Experimental liver injury models have demonstrated the important roles of this molecule: intraperitoneal administration of agonistic anti-Fas antibodies damages the liver by massive hepatocyte apoptosis, with (11) or without lethality (12). The involvement of the Fas/Fas-L system has been shown in many injury models (10). In addition, involvement of the Fas/Fas-L system in liver homeostasis was suggested by a report that Fas-deficient mice show substantial liver hyperplasia (13).

The Fas protein CD95 is a homotrimeric molecule and Fas-L causes clustering of the Fas death domains. An adapter protein called FADD then binds through its own death domain to the clustered receptor death domains (14). Upon recruitment by FADD, caspase-8 is activated through self-cleavage and oligomerization (15). Caspase-8 then activates downstream effector caspases such as caspase-3/7 (16). Bcl-2 family proteins play a pivotal role in controlling cell life and death, with some members such as Bcl-2 and Bcl-XL, inhibiting apoptosis, and others such as Bax, inducing cell death (17).

In the present paper, we establish an experimental system in which the effects of HCV protein on Fas-mediated apoptosis can be examined. Some of the potential target pathways of HCV proteins are also characterized.


    EXPERIMENTAL PROCEDURES
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Expression of HCV Transgene in Vivo-- HCV transgenic mice CN2 (BALB/c, 9-13 weeks old) were used in experiments. The CN2 transgenic mouse is the HCV genotype 1b transgenic mouse regulated by the Cre/loxP conditional switching system (8). AxCANCre and AxCAw1 recombinant adenoviruses were replication-deficient, lacking the E1A, E1B, and E3 regions (18). AxCANCre expressed recombinase Cre, and AxCAw1, which was used as the control adenovirus, lacked the inserted cre gene. AxCANCre or AxCAw1 (2 × 109 pfu) were intravenously injected into transgenic or nontransgenic female mice between 9 and 13 weeks of age. The CN2-8 mice were intravenously injected with AxCANCre and AxCAw1 virus and killed at 1, 2, 3, 4, 5, 6, and 7 days after the injection. The liver tissue was stained with anti-core polyclonal antibody RR8 using TSA direct method (PerkinElmer Life Sciences). Four days after the inoculation of recombinant adenovirus, mice were administered anti-Fas antibody (clone Jo2; PharMingen) intravenously, at a dose of 0.14 µg/g. The animals (6-10 mice) were observed for mortality over 24 h, and sacrificed 1, 2, 3, 4, 5, 6, 8, 12, and 24 h after anti-Fas antibody administration for liver histology and biochemical studies. Animal care was in accordance with institutional guidelines.

Quantification of HCV Core Protein in Mouse Liver-- Mouse liver was homogenized in 1 ml of RIPA buffer (1% SDS, 0.5% Nonidet P-40, 10 mM Tris (pH 7.4), 0.15 M NaCl). HCV core protein concentration of the liver lysates was quantified by fluorescent enzyme immunoassay (FEIA) (19). The FEIA is based on two high-affinity monoclonal antibodies directed against the HCV core protein. These two monoclonal antibodies, 5E3 and 5F11, recognize amino acids 21-40 and 41-60, respectively. These pretreated samples were added to EIA tubes precoated with monoclonal antibody 5F11. The tube was incubated for 10 min at 37 °C. Monoclonal antibody 5E3 conjugated to beta -D-galactosidase was added after washing with buffers, and the mixture was incubated for 9 min at 37 °C. 4-Methylumbelliferyl beta -D-galactopyranoside was used as a substrate. After incubation for 9 min at 37 °C, the relative fluorescence intensity was determined using a fluorometer after addition of 0.1 M glycine-NaOH (pH 10.3). HCV core protein concentration in the liver samples was divided by the total protein concentration and expressed as nanograms/mg of total protein.

Biochemical Analysis of Mouse Sera-- Blood samples were collected from the supra orbital veins at 0, 1, 2, 3, 4, 5, and 6 h after Fas antibody administration or heart puncturing of sacrificed mice, and centrifuged at 10,000 × g at 4 °C. Serum alanine aminotransferase (ALT) levels were assayed by enzyme reaction with Transnase Nissui kit (Nissui Pharmaceutical, Tokyo, Japan).

Measurement of Caspase Activity by Cleavage of Fluorogenic Substrates-- The cytosolic fraction of liver tissues was isolated as previously reported, with partial modification (20). Briefly, liver tissue was homogenized in a Dounce glass homogenizer (loose type) with lysis buffer (0.3 M mannitol, 5 mM MOPS, 1 mM EGTA, 4 mM KH2PO4, 20 µg/ml leupeptin, 10 µg/ml pepstatin A, 10 µg/ml aprotinin, 2 mM phenylmethylsulfonyl fluoride) and fractionated into pellet, heavy membrane, light membrane, and cytosolic fractions. The lysates were centrifuged at 600 × g for 10 min at 4 °C to remove debris or fiber. The supernatants were centrifuged at 10,000 × g for 15 min at 4 °C and the pellets collected as the heavy membrane fraction. The supernatants were again centrifuged at 100,000 × g for 45 min at 4 °C and collected as the cytosolic fraction. The activities of caspase-8, -9, and -3/7 were measured using Ac-LETD-AFC, Ac-LQTD-AFC, Ac-LEHD-AFC, or Ac-DEVD-AFC (Enzyme Systems Products) as substrates, respectively. Substrates were preincubated for 10 min at 37 °C in reaction buffer composed of 20 µM substrate, 100 mM Hepes (pH 7.5), 10% sucrose, 10 mM dithiothreitol (pH 7.5), and 0.1% CHAPS (21). After preincubation, 100 µg of lysate was added to 1.25 ml of reaction buffer and incubated for 60 min at 37 °C. The fluorescence of released 7-amino-4-trifluoromethylcoumarin (AFC) was detected by a fluorescence spectrometer (model F2000; Hitachi). Excitation wavelength was 400 nm and emission wavelength was 505 nm. Specific caspase-3/7 or -8 activity was determined by subtracting the values obtained in the presence of 10 µM Ac-DEVD-CHO or Ac-IETD-CHO (Peptide Institute, Osaka, Japan), inhibitors of caspase-3/7 or -8, respectively. One unit corresponds to the activity required to cleave 1 pmol of the substrate in 60 min.

Western Blotting of Caspase-8 and Bcl-2 Family Members-- The liver was perfused with phosphate-buffered saline(-) at 37 °C through the portal vein to remove monocytes, sectioned, and rapidly frozen in liquid nitrogen. Liver tissue was homogenized in a Dounce glass homogenizer using five complete up and down cycles of a glass loose-type pestle in a homogenate buffer composed of 1% SDS, 0.5% Nonidet P-40, 0.15 M NaCl, 10 mM Tris (pH 7.4), and 1 × complete protease inhibitor mixture (Roche Molecular Biochemicals). The lysates were centrifuged at 10,000 × g for 15 min at 4 °C. The supernatant was collected and used as liver lysate. Lysates (30 or 50 µg) were separated via 15% SDS-polyacrylamide gel electrophoresis using Tris Tricine buffer (Daiichi Pure Chemicals) and transferred onto polyvinylidene difluoride membrane (Amersham Pharmacia Biotech). The membrane was incubated in blocking buffer composed of 5% skim milk in TBST (20 mM Tris (pH 7.4), 137 mM NaCl, 0.5% Tween 20) followed by primary antibody; anti-Caspase-8 (T-16, Santa Cruz Biotechnology), anti-Bcl-XL (S-18, Santa Cruz Biotechnology), Bid (R&D Systems), Bax (N-20, Santa Cruz Biotechnology), Bcl-2 (4C-11, Santa Cruz Biotechnology), and Bad (New England Biolabs, Inc.).

Western Blotting of Cytochrome c-- Liver tissue was minced and washed once in ice-cold homogenizing buffer composed of 0.1% bovine serum albumin, 1 mM EGTA, 300 mM sucrose, 5 mM MOPS, 5 mM KH2PO4 (pH 7.4), and 1 × complete inhibitor mixture (Roche Molecular Biochemicals). The minced liver was gently homogenized in the homogenate buffer in a Dounce glass homogenizer using five complete up and down cycles of a glass loose-type pestle (22). The homogenate was centrifuged at 600 × g for 15 min at 4 °C. The supernatant was recovered and centrifuged at 10,000 × g for 15 min at 4 °C. The pellet, representing the heavy membrane fraction, was washed once with homogenization buffer and denatured with SDS sample buffer (67 mM Tris (pH 6.8), 2% SDS, 10% glycerol, 5% 2-mercaptoethanol). The protein concentration of the lysates was measured using the Bradford method (Bio-Rad DC protein assay). Lysates (30 µg) were electrophoresed by 15% SDS-polyacrylamide gel electrophoresis using Tris Tricine Buffer (Daiichi Pure Chemicals), transferred to polyvinylidene difluoride membrane (Amersham Pharmacia Biotech), and blocked with 5% skim milk in TBST. The membrane was washed with TBST for 5 min and incubated with anti-cytochrome c rabbit polyclonal antibody2 in 5% skim milk in TBST for 16 h at 4 °C. The membrane was washed with TBST three times and incubated with anti-rabbit IgG (Amersham Life Science) for 2 h at room temperature. The signal was detected by the ECL system (Amersham Pharmacia Biotech).

Histology and Immunohistochemical Staining-- Histopathological analysis of hematoxylin- and eosin-stained tissue sections was undertaken. The liver tissues were frozen with O.C.T. compound (Tissue Tech) for immunohistochemical analysis. The sections were fixed with a 1:1 solution of acetone:methanol at -20 °C for 10 min and then washed with phosphate-buffered saline(-). Subsequently, the sections were incubated with the IgG fraction of an anti-HCV core rabbit polyclonal antibody (RR8) (8) in blocking buffer for 16 h at 4 °C. The sections were incubated with secondary antibody, horseradish peroxidase-conjugated anti-rabbit IgG, for 2 h at room temperature. Immunohistochemical staining was completed using tyramide signal amplification (PerkinElmer Life Sciences).

For cytochrome c staining, the sections were incubated in blocking buffer for 30 min at room temperature and incubated with rabbit anti-cytochrome c antibodies for 3 h at room temperature. The sections were washed with phosphate-buffered saline(-). Primary antibodies were detected by Texas Red-conjugated anti-rabbit IgG (CAPPEL). Fluorescently labeled sections were stained with 0.5 µg/ml Hoechst 33342 dye (Molecular Probes) for 1 min at room temperature before coverslipping to stain cell nuclei. Fluorescence was observed under a fluorescence microscope (Carl Zeiss).

FACS Analysis-- Cytofluorometry was performed by FACS Calibur (Becton-Dickinson, San Jose, CA). Primary hepatocytes were stained with an optimal dilution of anti-Fas-FITC antibody (06134D, PharMingen, San Diego, CA), and examined by FACScan.

Statistical Analysis-- Data represent the mean ± S.D. All statistical analysis was performed using Student's t test. Statistical significance was established at p < 0.05.


    RESULTS
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

HCV Protein Expression in the Liver of HCV Transgenic Mice-- HCV proteins were expressed in the liver of HCV transgenic mice. Expression of core protein in AxCANCre adenovirus-injected CN2-8 transgenic mice liver was confirmed by immunofluorescence staining. Core protein was expressed in 50-60% of hepatocytes in the lobule of the liver section at day 4, and 70-80% of hepatocytes at 7 days after AxCANCre injection (Fig. 1A). The genomic DNA was prepared from liver 4 or 5 days after the injection of adenovirus and analyzed by Southern blot analysis. Transgene recombination occurred in the livers of the transgenic mice (Data not shown). In contrast, core protein was not expressed in liver sections from transgenic mice without AxCANCre injection or in AxCAw1-injected CN2 mice (Fig. 1A). The core protein levels in hepatocytes were measured by FEIA. The mean level of core protein was 5.7 ng/mg total protein in the HCV transgenic mice liver 4 days after the administration of AxCANCre adenovirus (Fig. 1B). The expression of core, E1 and E2 proteins was detected in AxCANCre-injected mouse liver by Western blot analysis (data not shown).



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Fig. 1.   Immunofluorescence analysis of HCV core protein in liver. A, liver sections of CN2-8 mice were fixed and stained using rabbit anti-core polyclonal antibodies as described under "Experimental Procedures." The CN2 mice (n = 3) were sacrificed 0, 1, 2, 3, 4, 5, 6, and 7 days after AxCANCre injection (HCV expressed). The control adenovirus AxCAw1-injected livers were examined on day 4 (HCV not expressed). The immunohistochemistry of HCV protein expressing hepatocytes was assayed by staining with anti-core antibody. B, core protein levels in hepatocytes of AxCANCre-injected CN2-8 mice were quantified by FEIA. The quantity of core protein shown represents the mean and S.D. of three individual experiments. C, the serum ALT level after injection of AxCANCre adenovirus in CN2-8 mice (mean and S.D. of three individual experiments).

The livers of CN2 mice, 5 days after administration of AxCANCre and AxCAw1, did not show infiltration of mononuclear cells or an increase in serum ALT levels (Fig. 1C). A significant liver injury was not evoked until day 5. Therefore, 4 days after the administration of AxCANCre or AxCAw1 was a suitable time point to characterize the effect of HCV proteins on Fas-mediated cell death.

Inhibition of Fas-mediated Death by HCV Proteins-- Mice not expressing HCV were treated with 0.14 µg/g mice body weight of anti-Fas antibody, which killed 50% of the mice over the initial 24 h (n = 6). In contrast, all of the HCV protein expressing transgenic mice were resistant to Fas stimulated lethality over the initial 24 h (Fig. 2A). Another transgenic mice strain, CN2-29 (8) was also analyzed to better understand the effects of the transgene integration site. CN2-29 mice expressed an average of 0.54 µg/mg core protein on day 4 after AxCANCre administration. Ten of the AxCAw1 adenovirus-injected CN2-29 mice were administered 0.14 µg/g anti-Fas antibody and observed for 24 h (Fig. 2B). Half of the AxCAw1 adenovirus-infected CN2-29 mice died. In contrast, 80% of the AxCANCre adenovirus-injected CN2-29 mice survived after the administration of anti-Fas antibody (n = 10). To investigate the effects of adenovirus, AxCANCre and AxCAw1 adenovirus-injected nontransgenic BALB/c mice were administered 0.14 µg/g anti-Fas antibody. Within 24 h of administration, 50% AxCAw1 and 50% AxCANCre injected nontransgenic BALB/c mice died (n = 10) (Fig. 2C). Expression of HCV proteins appeared to inhibit the progression of death after the injection of anti-Fas antibody.



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Fig. 2.   Survival rates of HCV transgenic mice after administration of anti-Fas antibody. Survival rate of CN2-8 (A), CN2-29 (B), or BALB/c nontransgenic mice (-) injected with AxCANCre (closed circle) or AxCAw1 (open circle) in response to an intravenous injection of 0.14 µg/g anti-Fas antibody.

Inhibition of Hepatic Injury in Transgenic Mice with HCV Protein Expression-- Serum ALT levels dramatically increased within 3 h of injection of the anti-Fas antibody in AxCAw1-injected CN2-8 mice (Fig. 3A). In contrast, significantly lower ALT levels were observed in HCV protein expressing CN2-8 transgenic mice 3 and 6 h after injection of the anti-Fas antibody (p = 0.0352, and p = 0.0266 respectively).



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Fig. 3.   Serum ALT level and histological analysis of liver after Fas antibody administration. A, serum ALT levels of Fas antibody injected HCV nonexpressing (open circles) and expressing (closed circle) mice. ALT levels represent the mean and S.D. of three individual experiments. B, hematoxylin and eosin staining of liver sections from transgenic mice, pretreatment and 4 h after anti-Fas antibody injection.

Liver tissue of anti-Fas antibody-treated mice was sampled from 0 to 4 h after the intravenous injection for histological examination (Fig. 3B). Four h after the anti-Fas antibody injection, hepatic injury was observed predominantly in the liver of mice not expressing HCV proteins (Fig. 3B) and fragmented nuclei were observed in over 80% of the hepatocytes. In contrast, histological analysis of livers from HCV protein expressing transgenic mice revealed that liver injury was suppressed and cell death was far less prevalent than in the liver of mice not expressing HCV proteins (Fig. 3B). The nuclear shrinkage and fragmentation observed in hepatic cell death resembled apoptotic cell death. Therefore, DNA fragmentation was assessed in the livers of anti-Fas antibody injected mice by genomic DNA laddering. Ladder formation was much more moderate in the HCV protein expressing transgenic hepatocytes than in the hepatocytes in the HCV-nonexpressing mice at 5, 6, 8, and 12 h after the injection of anti-Fas antibody (Fig. 4). These findings indicate that expression of HCV proteins inhibited the progress of apoptotic hepatocyte death caused by anti-Fas antibody injection.



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Fig. 4.   Genomic DNA fragmentation in HCV-negative (A) or HCV-protein expressing (B) hepatocytes. AxCAw1-injected (A) and AxCANCre-injected (B) CN2-8 mice were administered anti-Fas antibody on day 4 after adenovirus injection. Genomic DNA was prepared from livers at 0, 1, 2, 3, 4, 5, 6, 8, 12, and 24 h after anti-Fas antibody injection as described under "Experimental Procedures".

Expression of HCV Proteins Inhibited Activation of Caspase-3/7 and Caspase-9-- To clarify the mechanism of Fas-mediated apoptosis inhibition by HCV proteins, expression of Fas antigen on hepatocytes and activation of caspase-8, -9, and -3/7 proteases were examined in liver samples. Four h after the administration of anti-Fas antibody, Fas protein levels in hepatocytes and expression on the cell surface did not differ significantly between HCV-positive and HCV-negative mice (Fig. 5, A and B).



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Fig. 5.   A, expression of Fas protein after administration of anti-Fas antibody in the presence or absence of HCV protein was analyzed by Western blotting. B, FACS analysis of Fas expression on the surface of primary hepatocytes in the presence or absence of HCV, before anti-Fas antibody administration.

Caspase-8 was similarly activated within 1 h in both HCV-negative and HCV-positive transgenic mice treated with anti-Fas antibody (Fig. 6). The active form of the caspase-8 subunit (p18) appeared within 1 h after anti-Fas antibody injection (Fig. 6A). No significant difference was observed in p18 patterns between HCV protein expressing and nonexpressing CN2-8 mice (Fig. 6A). To determine the activity of caspase-8, -9, and -3/7, the fluorogenic substrates with the cleavage site by each caspase were used. The results were consistent with the results from Western blot analysis (Fig. 6A). Caspase-8 was similarly activated in the liver tissue of HCV protein expressing and nonexpressing mice within 1 h after anti-Fas antibody injection (Fig. 6B). HCV proteins expression did not repress substrate cleavage activity by caspase-8 as shown by the substrate cleavage assay using both Ac-LETD-AFC and Ac-LQTD-AFC, which are the known cleavage sites of Bid (22). After 3 and 4 h of Fas antibody administration, caspase-8 activity decreased in liver tissues from mice not expressing HCV proteins (Fig. 6B). The results suggested that the greater damage to liver tissue observed in mice not expressing HCV proteins contributed to the decrease in caspase-8 activity itself in HCV-negative livers. In contrast, activation of caspase-3/7 and -9 was suppressed in HCV protein expressing liver tissue, 3 and 4 h after antibody administration (Fig. 6B).



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Fig. 6.   A, analysis of caspase-8 in hepatocytes by Western blotting after injection of anti-Fas antibody. Migration positions of full-length (Procaspase-8, 57 kDa), cleaved intermediate (p44 and p46), and the activated subunit (p18) of caspase-8 are indicated. B, the time course of caspase activity of hepatocytes in AxCANCre- or AxCAw1-injected CN2-8 mice after treatment with anti-Fas antibody. Caspase activity was measured in cytosolic fractions prepared from hepatocytes 0, 1, 2, 3, and 4 h after injection of anti-Fas antibody (HCV not expressed, open bars; HCV expressed, black bars). Caspase and Bid cleavage activities were measured by release of AFC from peptide substrates. Units represent the mean and S.D. of three individual experiments.

Immunoblot Analysis of Bcl-2 Family Proteins in Hepatocytes of Mice Injected with Anti-Fas Antibody-- The inhibitory mechanism of Fas-mediated cell death by HCV genome expression was analyzed further. We examined expression levels of the Bcl-2 protein family, which localize in the mitochondria and regulate the progression of apoptotic cell death. Bcl-2 family protein level, including Bid, Bcl-XL, Bcl-2, Bad, and Bax was analyzed by immunoblot (Fig. 7, A and B). Caspase-8 activity of Bid cleavage was not inhibited by HCV proteins. Translocation of the C-terminal of Bid protein into mitochondria was not repressed by HCV proteins 4 h after the administration of anti-Fas antibody in HCV transgenic mice (Fig. 7A). Bcl-2 protein increased gradually after the administration of anti-Fas antibody in liver tissue of both HCV protein expressing and nonexpressing mice (Fig. 7B). Bcl-XL protein was consistently expressed in liver tissue of HCV protein expressing mice after 3 h (Fig. 7B). In contrast, the Bcl-XL protein decreased to the basal level over 3 h in nonexpressing mouse liver tissue (Fig. 7B). HCV protein did not contribute to the degradation of Bad protein after anti-Fas antibody-mediated cell death. Bax was consistently expressed even after the administration of anti-Fas antibody in both HCV protein expressing and nonexpressing mice.



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Fig. 7.   A, Bid was analyzed by Western blotting of heavy membrane and cytosolic fractions of transgenic mice livers at 0, 1, 3, and 4 h after injection of anti-Fas antibody. B, Western blot analysis of Bcl-2 family proteins (Bcl-2, Bad, Bcl-XL, and Bax) in hepatocytes of anti-Fas antibody injected mice. C, subcellular distribution of Bax protein in the liver after anti-Fas antibody injection. Bax was analyzed by immunoblot of cytosol (soluble fraction, S), plasma membrane (light membrane fraction, LM), mitochondria-rich (heavy membrane fraction, HM), and nuclear, (contained nuclei and some mitochondria; low speed pellet, P1) fractions of livers from transgenic mice, pretreatment and after anti-Fas antibody injection.

Bax protein level was analyzed by Western blot in four fractions, including the soluble, light membrane, heavy membrane (mitochondria rich), and low speed pellet fractions in liver lysates taken 0, 1, 2, 3, and 4 h after the administration of anti-Fas antibody. Bax protein translocated similarly from the cytosolic fraction to the mitochondria-rich fraction within 1 h of the administration of anti-Fas antibody in both HCV nonexpressing and expressing mice (Fig. 7C). This translocation of Bax has also been reported in some cell lines (23).

Inhibition of Cytochrome c Release by Expression of HCV Proteins-- To clarify HCV mediated-inhibitory mechanisms on the Apaf-1/caspase-9 amplification loop, the distribution pattern of cytochrome c was examined by immunohistochemistry. The originally prepared anti-cytochrome c antibody3 strongly detected cytochrome c in cytoplasm released from mitochondria, and weakly detected cytochrome c in mitochondria in a spotty pattern. Liver sections of pretreated mice showed a spotty staining pattern in the cytoplasm (Fig. 8A). Immunoreactivity of cytoplasmic cytochrome c increased in HCV-negative hepatocytes after 4 h of Fas antibody administration (Fig. 8A), reflecting the release of cytochrome c from mitochondria. In contrast, staining of cytochrome c was much weaker in HCV protein expressing hepatocytes (Fig. 8A). Therefore, HCV protein inhibited the release of cytochrome c from mitochondria.



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Fig. 8.   A, liver sections from HCV protein expressing and nonexpressing mice at 0 and 4 h after anti-Fas antibody injection were stained with anti-cytochrome c antibody. B, cytochrome c (Cyt. c) was analyzed by Western blotting of the heavy membrane fractions (HM) from transgenic mice livers. C, cytochrome c was analyzed by Western blotting of the cytosolic fractions (Cytosol) from transgenic mice livers. The quantification of detected cytochrome c by Western blotting with chemiluminescence assay was performed with a Chemi Doc (Bio-Rad) and relative intensity of each band was indicated.

To further examine the localization of cytochrome c, the liver lysates were fractionated by centrifugation into supernatant, heavy membrane, and nuclear fractions. The heavy membrane fraction was a mitochondria-rich fraction that contained cytochrome c, as indicated by immunoblot analysis with anti-cytochrome c antibody (Fig. 8B). The mitochondrial fraction of liver tissue was collected 0, 1, 3, and 4 h after anti-Fas antibody administration from both HCV protein expressing and nonexpressing mice.

The quantity of cytochrome c in the heavy membrane fraction of HCV protein expressing mice liver after 4 h of Fas antibody administration was not significantly reduced compared with HCV nonexpressing mice (Fig. 8B). Hence, the cytoplasmic fraction after both 3 and 4 h in HCV protein expressing mice liver had 3-4-fold decrease of cytochrome c than that of hepatocytes in HCV nonexpressing mice (Fig. 8C). These results indicated that the release of cytochrome c from mitochondria to cytoplasm in hepatocytes was suppressed by HCV protein expression.


    DISCUSSION
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

A part of the defense mechanism against virus infection is induced to initiate apoptotic cell death by signals delivered from CTL. On the other hand, numerous viruses have been reported to escape from apoptotic mechanism to maintain persistent infection (24-28). In this study, we investigated that HCV might cause persistent infection, as a result of suppression of Fas-mediated cell death and inhibition of HCV-infected hepatocyte rejection in the liver (Fig. 9).



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Fig. 9.   The model of the HCV protein target in the signaling pathway of Fas-mediated apoptosis. Two discrete apoptotic signaling pathways, a mitochondrial pathway dependent on cytochrome c/Apaf-1/caspase-9, and another signaling pathway involving direct activation of caspase-3, -6, and -7 (30, 35) are shown. Expression of HCV proteins may directly or indirectly suppress the release of cytochrome c from mitochondrial intermembrane.

Some reports indicated that the HCV core protein has antiapoptotic (1, 2, 5-7) and proapoptotic (3, 4) functions. The reason for the discrepancy among these reports is still unclear. Moreover, NF-kappa B has an antiapoptotic function against anti-Fas antibody and TNF-alpha -induced cell death. It was recently shown that HCV core protein activated NF-kappa B-associated signal (5-7). It may be that the core has multifunctional roles in the apoptotic signaling. This discrepancy may be explained by the possibility that it was caused by using established cell lines and clonically selected permanent transfectant cells in the previous studies. To avoid the artificial effects in cell lines and transgenic mouse with persistent expression of HCV protein, we examined the transgenic mouse using conditional transgene expression system (8). These hepatocytes of transgenic mouse retain original sensitivity to the apoptosis mediated by Fas signal. The conditional transgene expressed mouse makes it possible to examine native effect of HCV protein to Fas-mediated apoptosis.

The results of the present study revealed that Fas-mediated apoptotic cell death was suppressed by the expression of HCV proteins (core, E1, E2, and NS2) in vivo. In the presence of HCV proteins, lethality after the administration of anti-Fas antibody significantly decreased (Fig. 2). The survival rate correlated to HCV protein expression levels in transgenic mice (CN2-8 and 2-29) (Fig. 2, A and B).

These results revealed that apoptosis was inhibited by HCV protein expression, as shown by DNA ladder examination of mouse liver (Fig. 4). Expression of Fas on the surface of hepatocytes did not differ significantly among AxCANCre- or AxCAw1-injected mice and untreated mice (Fig. 5). Therefore, the downstream target of Fas signaling pathway was characterized. Many caspases were involved in the downstream of Fas signaling pathway (Fig. 9). Each caspase has a specific function, for example, as an initiator or effector. The initial caspase-8 cascade was activated in both HCV protein expressing mice and nonexpressing mice (Fig. 6, A and B). Caspase-9 and -3/7 activities were down-regulated in liver tissue of HCV protein expressing mice (Fig. 6). Apoptosis of hepatocytes requires effector caspases such as caspase-3/7 (16, 29). These results suggest that caspase-9 and -3/7 activation were inhibited by HCV proteins and/or that the mitochondria amplification loop of the Fas-mediated signaling pathway is inhibited by HCV proteins (Fig. 9).

Bcl-2 family proteins are involved in progression of apoptotic cell death in response to Fas signal (30). Bid is likely the activator of the mitochondrial pathway through cleavage by caspase-8 and translocation into the mitochondria. Bid then interacts with Bcl-2 and/or Bax protein causing the release of cytochrome c from mitochondria into the cytoplasm (31, 32). In the present study, distribution of Bid protein in the cytosol and heavy membrane fraction did not differ significantly between HCV protein expressing and nonexpressing mice (Fig. 7A). Therefore, the Bid pathway was not influenced by HCV protein. Bcl-2, Bad, and Bax protein quantities did not differ between HCV protein expressing and nonexpressing mice (Fig. 7, A and B). On the other hand, in the presence of HCV protein, Bcl-XL protein did not decrease 3 and 4 h after the administration of Fas antibody. This may have resulted from the down-regulation of caspase-3/7 activity (Fig. 7A) (16, 29). Therefore, the Bcl-2 family may not be significantly influenced by HCV proteins.

Immunohistochemical analysis of affected liver regions confirmed the release of cytochrome c from the mitochondria of HCV nonexpressing mice and expressing mice by anti-Fas antibody administration. The release of cytochrome c from the mitochondria to cytoplasm was suppressed in the HCV protein expressing mice liver compared with the HCV nonexpressing mice liver (Fig. 8A). Consistent with this result, cytochrome c released 5.2-6.6-fold increase from heavy membrane fraction to cytoplasmic fraction in the HCV nonexpressing liver tissue after 3 and 4 h of Fas antibody administration (Fig. 8, B and C). In contrast, cytochrome c was mainly present in the heavy membrane fraction, and the release of cytochrome c from the mitochondria into the cytoplasm was suppressed in the presence of HCV after 3 and 4 h of Fas antibody administration (Fig. 8, B and C). These results indicated that the release of cytochrome c from mitochondria to cytoplasm was inhibited by the expression of HCV proteins in transgenic mice. The inhibition of the activation of effector caspase-3/7 resulted from the suppression of the cytochrome c/Apaf-1/caspase-9 amplification loop by HCV and decreased lethality after Fas antibody administration. Further experiments are needed to determine whether the inhibition of cytochrome c from mitochondoria is due to the direct interaction of particular HCV proteins with a cellular protein. Or, it is possible that the interaction of expression of HCV E1 and E2 glycoproteins may induce a more general cellular response, stimulating chaperones and delaying the entry into apoptosis (33, 34). Further elucidation of the apoptotic signaling functions of HCV will not only advance the understanding of molecular mechanisms of HCV pathogenesis, but also shed light on the basic mechanism of apoptosis.


    ACKNOWLEDGEMENTS

We express our gratitude to Tadatsugu Taniguchi for support of this study and Kazuaki Inoue and Kentaro Tomita for advice on histology. We thank Masao Miwa, Otoya Ueda, and Mitugu Takahashi for breeding the transgenic mice.


    FOOTNOTES

* This work was supported in part by a research fellowship from the Japan Society for the Promotion of Science, a grant-in-aid for Specially Promoted Research on Viral Diseases from the Tokyo Metropolitan Government, a grant from the Ministry of Education, Science, and Culture of Japan, and a grant from the Ministry of Health and Welfare of Japan.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|||| To whom correspondence should be addressed: Dept. of Microbiology and Cell Biology, The Tokyo Metropolitan Institute of Medical Science, 3-18-22, Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan. Tel.: 81-3-3823-2101; Fax: 81-3-3828-8945; E-mail: mkohara@rinshoken.or.jp.

Published, JBC Papers in Press, January 18, 2001, DOI 10.1074/jbc.M010137200

2 F. Shibasaki, manuscript in preparation.

3 F. Shibasaki et al., manuscript in preparation.


    ABBREVIATIONS

The abbreviations used are: HCV, hepatitis C virus; ALT, alanine aminotransferase; FEIA, fluorescent enzyme immunoassay; Tricine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine; CHAPS, 3-[(3-cholamidopropyl)dimethylammoniol]-1-propanesulfonic acid; TSA, tyramide signal amplification; Ac-DEVD-AFC, Ac-Asp-Glu-Val-Asp-7-amino-4-(trifluoromethyl)-coumaride; NF-kappa B, nuclear factor-kappa B; MOPS, 4-morpholinepropanesulfonic acid; FACS, fluorescence-activated cell sorter; TNF-alpha , tumor necrosis factor-alpha .


    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES


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