Mechanism of Activation of ERK2 by Dual Phosphorylation*

Claudine N. ProwseDagger and John Lew§

From the Department of Molecular, Cellular and Developmental Biology, Interdepartmental Program in Biochemistry and Molecular Biology, University of California, Santa Barbara, California 93106 and the Dagger  Graduate Program in Biomedical Sciences, University of California, San Diego, California 92093

Received for publication, September 6, 2000



    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS AND DISCUSSION
REFERENCES

The mitogen-activated protein (MAP) kinases are characterized by their requirement for dual phosphorylation at a conserved threonine and tyrosine residue for catalytic activation. The structural consequences of dual-phosphorylation in the MAP kinase ERK2 (extracellular signal-regulated kinase 2) include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In this study, we report the specific effects of dual phosphorylation on the individual catalytic reaction steps in ERK2. Dual phosphorylation leads to an increase in overall catalytic efficiency and turnover rate of approximately 600,000- and 50,000-fold, respectively. Solvent viscosometric studies reveal moderate decreases in the equilibrium dissociation constants (Kd) for both ATP and myelin basic protein. However, the majority of the overall rate enhancement is due to an increase in the rate of the phosphoryl group transfer step by approximately 60,000-fold. By comparison, the rate of the same step in the ATPase reaction is enhanced only 2000-fold. This suggests that optimizing the position of the invariant residues Lys52 and Glu69, which stabilize the phosphates of ATP, accounts for only part of the enhanced rate of phosphoryl group transfer in the kinase reaction. Thus, significant stabilization of the protein phosphoacceptor group must also occur. Our results demonstrate similarities between the activation mechanisms of ERK2 and the cell cycle control enzyme, Cdk2 (cyclin-dependent kinase 2). Rather than dual phosphorylation, however, activation of the latter is controlled by cyclin binding followed by phosphorylation at Thr160.



    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS AND DISCUSSION
REFERENCES

Members of the family of protein kinases referred to as the MAP1 kinases are critical components of the biochemical processes that define the essence of life, the ability of cells to sense their external environment and respond. The prototype member of the MAP kinase family that mediates signaling by all polypeptide mitogens is the extracellular signal-regulated kinase, ERK2. Like all MAP kinases, ERK2 participates in a three-tier protein phosphorylation cascade that, in response to growth factor receptor signaling, is activated by dual phosphorylation catalyzed by an upstream activating kinase, MEK1/MEK2. Among the targets of ERK2 are downstream kinases involved in cellular growth control as well as nuclear transcription factors. Thus, ERK2 provides an essential link in transducing the diverse signals from transmembrane growth factor receptors into gene regulatory events (for review see Refs. 1 and 2).

The x-ray crystallographic structure of ERK2 (357 amino acids) reveals a conserved catalytic core (residues 22-311) flanked by N- and C-terminal extensions that lie on the surface of the molecule. Like all protein kinases, the catalytic core is globular, consisting of an N- and C-terminal lobe, whose interface defines the active site cleft. Within the active site, the adenine ring of ATP is deeply buried, with the gamma -phosphate pointing toward the mouth of the active site where protein substrates bind and where phosphotransfer occurs (3).

All protein kinases display near their active sites an "activation loop," the conformation of which must be optimized for high catalytic activity (4). For most, but not all, protein kinases, this activity is dependent upon phosphorylation at a single conserved residue located at the C-terminal end of the activation loop (4). In many cases, phosphorylation at this site is catalyzed by a heterologous kinase and constitutes a physiological mechanism for kinase regulation. For example, phosphorylation of the cyclin-dependent kinases at the conserved activation loop threonine (Thr160 in Cdk2) by a cdk activating kinase (CAK) is critical for regulation of cell cycle progression (5). Similarly, the MAP kinases are regulated by phosphorylation by their upstream activators, the MAPK/ERK kinases (MEKs), in response to extracellular signaling (2).

The hallmark of the MAP kinases is their unique requirement for dual phosphorylation at a conserved threonine and tyrosine residue belonging to the consensus sequence TXY for catalytic activation (6). In ERK2, these sites are Thr183 and Tyr185. The phosphorylation of both of these residues is catalyzed by the dual-specific upstream kinases MEK1 or MEK2 (2). The structural role of both phosphorylations in catalytic activation has been revealed by comparison of the crystallographic structures of (non-p)ERK2 (3) and (pp)ERK2 (7). The major alteration upon dual phosphorylation is a closure of the active site cleft, which results in optimal alignment of the key catalytic residues that contact the phosphate groups of ATP. In addition, there is significant remodeling of the activation loop as well as the P+1 surface pocket,2 the latter of which is necessary to accept the P+1 proline residue essential for the recognition of all ERK substrates.

Given the structural consequences of dual phosphorylation revealed by x-ray crystallography, it is not known how such changes in structure specifically affect the kinetics of individual reaction steps along the catalytic reaction pathway. Thus, a correlation between structure and mechanism, and therefore regulation, cannot be made. In this study, the catalytic reaction pathway for nonphosphorylated ERK2 was determined and compared with that of the fully active, dual-phosphorylated enzyme. The results provide a quantitative understanding of the mechanistic basis for catalytic activation by dual phosphorylation. Finally, our studies reveal functional similarities between ERK2 and Cdk2 in terms of their mechanisms of activation.


    EXPERIMENTAL PROCEDURES
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS AND DISCUSSION
REFERENCES

Materials-- All chemicals (KCl, EDTA, MgCl2, MOPS, sucrose, dithiothreitol) were purchased from Fisher, except for ATP (Sigma), and [gamma -32-P]ATP (ICN).

MBP Preparation-- Myelin basic protein (MBP) was purified from a bovine brain acetone powder (Sigma B0508) by acid extraction followed by cation exchange chromatography as described previously (8).

ERK2 Preparation-- Expression and purification of the recombinant rat ERK2 N-terminally fused to a hexahistidine tag was carried out as described previously (9). Briefly, ERK2 overexpressed in Escherichia coli was purified by Ni2+-NTA affinity chromatography followed by FPLC anion exchange chromatography on Uno Q (Bio-Rad). Select fractions from peak 1 (see "Results") were used for all experiments.

(pp)ERK2 was generated as described previously (8). (non-p)ERK2 purified by Ni2+-NTA affinity chromatography was subject to phosphorylation by recombinant MEK1 in vitro, and the dual-phosphorylated material was further purified by FPLC anion exchange chromatography on Uno Q (Bio-Rad). ERK2 was confirmed to be dual-phosphorylated by electrospray mass spectrometry. Both (non-p)ERK2 and (pp)ERK2, purified as described above, were essentially homogeneous based on analysis by SDS-polyacrylamide gel electrophoresis. The concentration of ERK2 was determined spectrophotometrically based on an extinction coefficient (epsilon 280 = 44, 825 cm-1 M-1) calculated from its primary amino acid sequence (10).

Kinase Assays and Data Analysis-- Kinase activity was monitored by a radioisotope assay in which the rate of incorporation of 32P from [gamma -32P]ATP into MBP was directly measured. Reactions were carried out in phosphorylation buffer (20 mM MOPS, pH 7.4, 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 10 mM MgCl2(total)) containing 3 µM ERK2 and varied concentrations of MBP. Reactions were initiated by the addition of [gamma -32P]ATP (300-500 cpm/pmol) at varied concentrations and allowed to proceed at 23 °C for 45 min, after which time they were terminated with 25% acetic acid. The 32P-labeled MBP product was resolved from unincorporated [gamma -32P]ATP by ascending chromatography on P81 phosphocellulose paper (Whatman) as described previously (8). Radioactivity was quantified by Cerenkov counting.

Steady-state kinetic parameters were determined by nonlinear least squares analysis of initial velocity data obtained from several concentrations of MBP at several fixed concentrations of ATP. The following equation (Equation 1) for a two-substrate sequential reaction was globally fit to the data using the program Scientist (Micromath Inc.),
<UP>v</UP>=<UP>V · A · B</UP>/(K<SUB>i<UP>A</UP></SUB> · K<SUB>m<UP>B</UP></SUB>+K<SUB>m<UP>B</UP></SUB><UP> · A</UP>+K<SUB>m<UP>A</UP></SUB><UP> · B</UP>+<UP>A · B</UP>) (Eq. 1)
where v is the initial velocity, V is the maximal initial velocity, A and B are the fixed and varied substrates, respectively, Km is the Michaelis constant, and KiA is the dissociation constant for A. kcat was determined by dividing the maximal initial velocity by the enzyme concentration.

Solvent Viscosometric Studies-- Steady-state assays, as described above, were carried out in buffer containing sucrose ranging from 0 to 40%, to give relative solvent viscosities ranging between 1 and 4.2. Relative solvent viscosity was determined as described previously (8).

ATPase Assays-- ATPase activity of (non-p)ERK2 was determined in a radioisotope assay in which the rate of 32P production was monitored. Reactions were performed in phosphorylation buffer in a total volume of 20 µl at 23 °C. Typically, reactions containing 3 µM (non-p)ERK2 were initiated by the addition of [gamma 32-P]ATP (500 cpm/pmol) at various concentrations, allowed to proceed for 2 h at 23 °C, then terminated in 1 ml of 0.1 N HCl. To determine the amount of phosphate produced, the stopped reactions were incubated with 200 µl of charcoal solution (10% activated charcoal (Sigma C-6289), 10% acetic acid, 2.5 mM KH2PO4) for 1 h on ice and then centrifuged at maximum speed in a microcentrifuge for 30 min. Radioactivity in the supernatant was quantified by counting 500 µl of the supernatant using the Cerenkov method.

ATPase activity of (pp)ERK2 was determined using a coupled spectrophotometric assay (11). The coupling reagents and their concentrations were as follows: 15 units/ml lactate dehydrogenase, 7.5 units/ml pyruvate kinase, 1 mM P-enolpyruvate, and 130 µM NADH. All reactions were performed in phosphorylation buffer in a total volume of 75 µl at 23 °C. Reactions were initiated by the addition of 1 µM (pp)ERK to the reaction mix containing ATP at various concentrations. Progress of the reaction was monitored by a continuous decrease in absorbance at 340 nm in a Shimadzu UV1601 spectrophotometer. Initial velocities in µM/min were calculated based on an extinction coefficient for NADH of 6220 cm-1 M-1at 340 nm. Measurement of ATPase activity of (pp)ERK2 by spectrophotometric or by radioisotope labeling methods gave identical results. Steady-state kinetic parameters for both (non-p)ERK2 and (pp)ERK2 were determined from nonlinear regression analysis of initial velocities as a function of ATP concentration using the Michaelis-Menten equation.

Experiments to determine solvent viscosity effects on kcat and kcat/Km for ATPase reactions were carried out under the following conditions: (non-p)ERK2, 2 mM and 800 µM ATP; (pp)ERK2, 4 mM and 90 µM ATP. Sucrose was varied between 0 and 40%.


    RESULTS AND DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS AND DISCUSSION
REFERENCES

Purification of (non-p)ERK2-- (non-p)ERK2 was expressed and purified from E. coli. Purification using anion exchange chromatography revealed two peaks of protein corresponding to ERK2, both of which displayed identical activities and activation properties. These observations are consistent with those reported previously during the crystallization of ERK2, in which only peak 1 formed crystals (9). Accordingly, we have used this fraction of ERK2 for all of our studies.

The activity of each fraction from peak 1 was determined. When assayed for kinase activity, it was found that the latter fractions of the peak exhibited substantially higher kinase activity than those eluting earlier (Fig. 1). The high specific activity associated with the later fractions may be attributable to enzyme that had undergone autophosphorylation during induction. In all our studies, however, fractions free of the high kinase activity (fractions 42-43, Fig. 1) were used. In these fractions, the profile of kinase activity corresponded to the ERK2 protein concentration exactly, and when subjected to phosphorylation by MEK1 the enzyme could be activated to a form that displayed high catalytic activity (kcat/Km = 1 µM-1 s-1 and kcat = 10 s-1) (8).



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Fig. 1.   Anion exchange chromatography of (non-p)ERK2. ERK2 was purified by Ni2+ chelate chromatography and then further purified by FPLC over UnoQ (Bio-Rad). The Uno Q trace is shown. Solid circles () correspond to A280. Bars correspond to kinase activity toward MBP, determined as follows: 5 µl of each fraction was incubated with 100 µM MBP and 1 mM [gamma -32P]ATP (300 cpm/pmol) in phosphorylation buffer (15 µl total volume) for 1 h, and total 32P-labeled MBP was produced was determined as described under "Experimental Procedures." Fractions 42 or 43 were used for all assays. The dashed line (- - - ) indicates the salt gradient, which runs from 100 mM (fraction 38) to 250 mM (fraction 52) NaCl.

Steady-state Kinetic Analysis-- The steady-state kinetic parameters for the phosphorylation of myelin basic protein by (non-p)ERK2 were determined. MBP phosphorylation was linear for at least 2 h, indicating that autoactivation of (non-p)ERK2 did not occur within this time frame. A data set of initial reaction velocities obtained under conditions of varied MBP concentrations at several fixed concentrations of ATP was analyzed. Fig. 2 shows the experimental data and the best-fit regression curves in double reciprocal form. The regression analysis yielded values of kcat = 2 × 10-4 s-1, Km(MBP) = 50 µM and Km(ATP) = 700 µM. The kcat value is down 50,000-fold from that of (pp)ERK2, whereas the Km values for MBP and ATP are each up by only 10-fold. The changes in the steady-state parameters correspond to an overall catalytic efficiency toward MBP (kcat/Km(MBP)), which is attenuated 600,000-fold.



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Fig. 2.   Steady-state kinetic analysis of MBP phosphorylation. Initial velocities obtained at various MBP concentrations and several fixed concentrations of ATP were determined by a radioisotope assay as described under "Experimental Procedures." A Michaelis-Menten model describing a two-substrate sequential addition was globally fit to the data by nonlinear regression analysis. The best-fit curves and experimental data are shown in double reciprocal form. The optimized kinetic parameters resulting from the regression analysis are reported in Table I. ATP was fixed at 4000 (diamond ), 3000 (triangle ), 2000 (black-square), 1000 (open circle ), and 500 µM (+) (from bottom to top). The concentration of (non-p)ERK2 was 3 µM.

Solvent Viscosometric Analysis-- The Michaelis-Menten parameters described above are a composite of microscopic rate constants combined in a manner dependent upon the order of substrate addition. The steady-state data for (non-p)ERK2 are consistent with both randomly and compulsorily ordered mechanisms. However, if the kinetic mechanism of this enzyme is ordered, it is necessarily ordered with ATP binding first. This is true because the crystal structure of (non-p)ERK2 has been obtained with bound ATP alone (3). In addition, (non-p)ERK2 displays measurable ATPase activity in the absence of MBP (see below).

Under saturating conditions of ATP, the catalytic mechanism of (non-p)ERK2 can therefore be described by Scheme 1. In this scheme, catalytic efficiency is given by [kcat/Km(MBP) k2·k3/(k-2 + k3)], whereas the turnover rate is given by [kcat = k3·k4/(k3 + k4)] (12). To solve for the microscopic constants, k2, k-2, k3, and k4, we employed steady-state solvent viscosometric techniques, which allow separation of the diffusive (k2, k-2, k4) from the nondiffusive (k3) reaction steps (13-15). Initial velocity data were obtained as a function of MBP concentration at several fixed concentrations of sucrose, and the effect of solvent viscosity on kcat or kcat/Km(MBP) was determined. Since it was not possible to carry out these experiments at a single, saturating concentration of ATP, because of the high Km(ATP) value, viscosity data were collected at several subsaturating ATP concentrations. The viscosity effect on appkcat (Fig. 3A) or appkcat/Km(MBP) (Fig. 3B) was determined at each ATP concentration by plotting the fold decrease in the respective rate parameter as a function of the relative solvent viscosity. The viscosity effect is given by the slopes of the best-fit lines in Fig. 3, A and B. The true viscosity effect on kcat and kcat/Km(MBP) was determined by extrapolation to infinite ATP concentration. We saw no significant viscosity effect on either rate parameter at any ATP concentration tested, and we therefore conclude that both kcat and kcat/Km(MBP) are insensitive to the relative solvent viscosity.



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Scheme 1.  



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Fig. 3.   Solvent viscosity effects on kcat and kcat/Km(MBP) for the phosphorylation of MBP. The "viscosity effect" on a given rate parameter is defined as the rate of change in reaction rate with respect to the relative solvent viscosity and corresponds to the slopes of the lines in panels A and B. The true viscosity effects on kcat (panel A) or kcat/Km(MBP) (panel B) were determined from the apparent rate values measured at three subsaturating concentrations of ATP (--- --- --- ---, 0.2 mM; ----------, 1 mM------open circle ------, 2 mM) as a function of increasing relative solvent viscosity, followed by extrapolation to infinite ATP concentration. In panel A, MBP was either varied or fixed at 600 µM. In panel B, MBP was fixed at 20 µM. The viscosity effects on kcat/Km (kmeta ) and kcat (kcateta ) relate to the individual rate constants in Scheme 1 as follows: kcat/Kmeta  = k3/(k-2 + k3); kcateta  = k3/(k3 + k4 (16). A value for kcat/Kmeta and kcateta approaching zero implies that k-2 k3 and k3 k4, respectively.

The viscosity effect (designated by superscripted eta ) on kcat/Km(MBP) is given by [kcat/Km(MBP)eta  = k3/(k-2 + k3)], whereas the viscosity effect on kcat is given by [kcateta  = k3/(k3 + k4)] (16). The kinetic constants derived from the viscosity studies are shown in Table I. The lack of viscosity effect on kcat implies that the overall rate of substrate turnover is limited by phosphoryl group transfer (k3) and that the diffusion of either product from the active site (k4) is not rate-limiting. The rate of phosphoryl group transfer can therefore be assigned a value of 0.012 min-1, which is 60,000-fold lower than that in (pp)ERK2.


                              
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Table I
Kinetic and thermodynamic parameters for (non-p)ERK2 versus (pp)ERK2

The viscosity effect on kcat/Km for ATP was also determined.3 No viscosity effect on this parameter was observed. Thus, the lack of a viscosity effect on both kcat/Km(MBP) and kcat/Km(ATP), as well as on kcat, supports a kinetic scheme in which both substrates exist in rapid equilibrium with the ternary Michaelis complex, whose breakdown to form products is entirely limited by phosphoryl group transfer. In such a model, the true affinities (Kds) of both substrates to form the ternary complex are given by their Km values. Thus, our steady-state data reveal that dual phosphorylation enhances the affinity of ATP binding by approximately 12-fold, whereas the affinity for MBP is enhanced >= 100-fold.

ATPase Activity-- An important consequence of dual-phosphorylation is the optimization of the alignment of the invariant residues, Lys52, which coordinates to the alpha - and beta -phosphates of ATP, and Glu69, which stabilizes Lys52, in (pp)ERK2 (7). To determine the relative contribution of these interactions to the overall increase in the phosphoryl group transfer step of the kinase reaction, we compared the fold increase in catalytic parameters of the kinase reaction to those of the ATPase reaction (Table I). The steady-state parameters and individual rate constants for the ATPase reaction for both (non-p)ERK2 and (pp)ERK2 were determined by solvent viscosometric analysis (see "Experimental Procedures"). Similar to the kinase reaction, the major effect of dual phosphorylation was a large (2000-fold) increase in the phosphotransfer rate, whereas the observed increase in ATP binding affinity was only 12-fold. However, the 2,000-fold increase in the rate of phosphotransfer is significantly less than the 60,000-fold rate enhancement of this step seen in the kinase reaction. This finding suggests that the net stabilization of the transition-state complex for phosphoryl group transfer to MBP occurs only in part by stabilization of the ATP moiety and that significant stabilization of the protein phosphoacceptor substrate moiety must also occur.

Mechanism of Activation by Dual Phosphorylation-- We previously characterized the kinetic reaction pathway of the dual-phosphorylated form of ERK2 (8). In comparison to (pp)ERK2, (non-p)ERK2 displays kcat/Km(MBP) and kcat values that are decreased by approximately 600,000- and 50,000-fold, respectively (Table I). Yet, the Km values for MBP and ATP are each increased by only 10-fold. Therefore, the exceedingly low activity of (non-p)ERK2 is due to the dramatically decreased rates of substrate capture and turnover and not the inability to saturate the enzyme with substrate under steady-state conditions.

The extreme rate enhancements caused by dual phosphorylation are the most dramatic of any protein kinase for which the kinetic basis for activation by phosphorylation has been investigated. The large increases in catalytic efficiency and turnover rate are attributable to an approximate 60,000-fold increase in the rate of phosphotransfer, 12-fold higher binding affinity for ATP, and a minimum 100-fold higher affinity for MBP. The ability to bind MBP more tightly correlates to a remodeling of the P+1 surface pocket that functions to bind the essential proline residue found in all ERK2 substrates; this is achieved in part by hydrogen bonding between the phosphoryl group oxygens of PO3-Tyr185 and the side chains of Arg189 and Arg192 (7). Although our data provides only a lower limit value on the absolute enhancement in substrate binding affinity, it should be noted that tighter binding of the substrate to (pp)ERK2 will not result in higher catalytic efficiency, because the rate of MBP binding to (pp)ERK2 already occurs at the diffusion-controlled limit (8). Thus, the catalytic efficiency of the dual-phosphorylated enzyme depends only on the rate of substrate encounter.

Dual phosphorylation results in a rotation of the N- and C-terminal lobes, which closes the active site cleft and optimizes the alignment of the essential catalytic residues, Lys52 and Glu69 (7). This results in a 12-fold tighter binding of ATP to the enzyme, and a 2000-fold increase in the rate of phosphoryl group transfer with respect to the ATPase reaction. Nonetheless, this increase in phosphotransfer rate is approximately 30-fold down from that seen in the kinase reaction, demonstrating that stabilization of the phosphate moieties of ATP accounts for only a portion of the overall enhanced rate of chemistry with respect to the phosphorylation of protein substrates.

Correlation of the ATPase and kinase activities of ERK2 reveals similarities in its mechanism of activation to that of Cdk2/cyclin A. For example, activation of both enzymes involves two steps. In Cdk2/cyclin A, these steps are cyclin binding followed by phosphorylation at Thr160 (5), whereas in ERK2, phosphorylation at Tyr185 followed by phosphorylation at Thr183 is required (6, 17). In Cdk2/cyclin A, the rate of phosphotransfer in the ATPase reaction is unchanged by phosphorylation at Thr160 (18). This is consistent with crystallographic information which shows that the role of phosphorylation at Thr160 is not to align the conserved lysine (Lys33) and glutamate (Glu51) side chains, thus stabilizing ATP. Instead, alignment of these residues in Cdk2 is achieved by the binding of cyclin. However, the rate of the same step in the kinase reaction of Cdk2/cyclin A is enhanced nearly 3000-fold (18). Thus, although cyclin binding serves to stabilize the ATP portion of the transition state for phosphoryl group transfer, phosphorylation at Thr160 serves to stabilize the protein phosphoacceptor group. Thr160 in Cdk2 and Thr183 in ERK2 occupy structurally analogous roles (4); both coordinate to a positively charged triad of arginine side chains that bridge the N- and C-terminal lobes of the catalytic cores. Thus, it is possible that phosphorylation at Thr183 in ERK2 may function analogously to that at Thr160 in Cdk2/cyclin A, whereas phosphorylation at Tyr185 in ERK2 may play a role analogous to cyclin binding.

In summary, we have demonstrated that dual phosphorylation of ERK2 results in 10-100-fold greater rate enhancements compared with other protein kinases in which activation is dependent on phosphorylation at only a single site. Most of the increase in catalytic power in ERK2 is attributable to an increased rate of phosphotransfer, which is coordinately accomplished by two independent mechanisms: 1) stabilization of the phosphate moieties of ATP via alignment of Lys52 and Glu69 and 2) stabilization of the protein phosphoacceptor group. In Cdk2, we propose that these may be separately achieved by cyclin binding and phosphorylation at Thr160, respectively (18). We hypothesize that a similar mechanism of activation may be functionally conserved in ERK2, except that stabilization of the ATP and protein phosphoacceptor groups may instead be controlled separately by phosphorylation at Tyr185 and Thr183.


    ACKNOWLEDGEMENTS

We are grateful to Dr. Larry Brunton, University of California, San Diego, Biomedical Sciences Program, for invaluable support and encouragement.


    FOOTNOTES

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed. Tel.: 805-893-5336; Fax: 805-893-4724; E-mail: lew@lifesci.ucsb.edu.

Published, JBC Papers in Press, October 2, 2000, DOI 10.1074/jbc.M008137200

2 P0 is the substrate phosphorylation site.

3 Initial velocities were determined at 1 mM MBP, 175 µM ATP, and varied sucrose. Identical conditions, except in 300 µM ATP, gave proportionally higher rates, indicative of true kcat/Km conditions.


    ABBREVIATIONS

The abbreviations used are: MAP, mitogen-activated protein; ERK2, extracellular signal-regulated kinase 2; (non-p)ERK2, nonphosphorylated ERK2; (pp)ERK2, dual-phosphorylated ERK2; Cdk, cyclin-dependent kinase; MBP, myelin basic protein; MOPS, 4-morpholinepropanesulfonic acid; FPLC, fast protein liquid chromatography.


    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS AND DISCUSSION
REFERENCES


1. Cobb, M. H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500[CrossRef][Medline] [Order article via Infotrieve]
2. Lewis, T. S., Shapiro, P. S., and Ahn, N. G. (1998) Adv. Cancer Res. 74, 49-139[Medline] [Order article via Infotrieve]
3. Zhang, F., Strand, A., Robbins, D., Cobb, M. H., and Goldsmith, E. J. (1994) Nature 367, 704-711[CrossRef][Medline] [Order article via Infotrieve]
4. Johnson, L. N., Noble, M. E., and Owen, D. J. (1996) Cell 85, 149-158[Medline] [Order article via Infotrieve]
5. Morgan, D. O. (1995) Nature 374, 131-134[CrossRef][Medline] [Order article via Infotrieve]
6. Cobb, M. H., and Goldsmith, E. J. (1995) J. Biol. Chem. 270, 14843-14846[Free Full Text]
7. Canagarajah, B. J., Khokhlatchev, A., Cobb, M. H., and Goldsmith, E. J. (1997) Cell 90, 859-869[Medline] [Order article via Infotrieve]
8. Prowse, C. N., Hagopian, J. C., Cobb, M. H., Ahn, N. G., and Lew, J. (2000) Biochemistry 39, 6258-6266[CrossRef][Medline] [Order article via Infotrieve]
9. Zhang, F., Robbins, D. J., Cobb, M. H., and Goldsmith, E. J. (1993) J. Mol. Biol. 233, 550-552[CrossRef][Medline] [Order article via Infotrieve]
10. Gill, S. C., and von Hippel, P. H. (1989) Anal. Biochem. 182, 319-326[Medline] [Order article via Infotrieve]
11. Cook, P. F., Neville, M. E., Jr., Vrana, K. E., Hartl, F. T., and Roskoski, R., Jr. (1982) Biochemistry 21, 5794-5799[Medline] [Order article via Infotrieve]
12. Cleland, W. W. (1975) Biochemistry 14, 3220-3224[Medline] [Order article via Infotrieve]
13. Brouwer, A. C., and Kirsch, J. F. (1982) Biochemistry 21, 1302-1307[Medline] [Order article via Infotrieve]
14. Caldwell, S. R., Newcomb, J. R., Schlecht, K. A., and Raushel, F. M. (1991) Biochemistry 30, 7438-7444[Medline] [Order article via Infotrieve]
15. Adams, J. A., and Taylor, S. S. (1992) Biochemistry 31, 8516-8522[Medline] [Order article via Infotrieve]
16. Nakatani, H., and Dunford, H. B. (1979) J. Am. Chem. Soc. 83, 2662-2665
17. Haystead, T. A., Dent, P., Wu, J., Haystead, C. M., and Sturgill, T. W. (1992) FEBS Lett. 306, 17-22[CrossRef][Medline] [Order article via Infotrieve]
18. Hagopian, J. C., Kirtley, M. P., Stevenson, L. M., Gergis, R. M., Russo, A. A., Pavletich, N. P., Parsons, S. M., and Lew, J. (2001) J. Biol. Chem. 276, 275-280[Abstract/Free Full Text]


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