CCR11 is a functional receptor for the monocyte chemoaattractant protein family of chemokines.

Vicki L. Schweickart, Angela Epp, Carol J. Raport, and Patrick W. Gray

In this paper, we reported that CCR11 is a functional receptor for the MCP family of chemokines. Gosling and colleagues (Gosling, J., Dairaghi, D. J., Wang, Y., Hanley, M., Talbot, D., Miao, Z., and Schall, T. J. (2000) J. Immunol. 164, 2851-2856) simultaneously reported ELC, SLC, and TECK to be the ligands for this receptor (which they called CCR10). Upon further investigation, the transfected cell line used for our original study was found to express undetectable levels of CCR11 by Northern analysis. To generate this cell line, we utilized the selective method of chemotaxis to enrich for cells that responded to chemokines. The selected cells responded to MCP-4 and other members of the MCP family as reported, but new Northern analysis data show an up-regulation of the endogenous murine CCR2 in these cells. Furthermore, cells transfected with recombinant murine CCR2 have a ligand recognition pattern and chemotactic response similar to our reported CCR11. Consequently, we believe that our observed data were not due to CCR11 but instead may be attributable to up-regulation of the endogenous murine CCR2 gene. We have since expressed CCR11 in both L1.2 and HEK293 cells and confirmed high levels of expression with a CCR11-specific antiserum. Both cell lines demonstrate binding to ELC, SLC, and TECK. Therefore, we are in agreement with the results of Gosling et al. and have confirmed ELC, SLC, and TECK as ligands for CCR11. Other information in our report regarding the sequence, chromosomal localization, and tissue distribution of the receptor is accurate and not affected by this correction.