Calcineurin Homologous Protein as an Essential Cofactor for Na+/H+ Exchangers*

Tianxiang Pang, Xiaohua Su, Shigeo WakabayashiDagger, and Munekazu Shigekawa

From the Department of Molecular Physiology, National Cardiovascular Center Research Institute, Fujishiro-dai 5-7-1, Suita, Osaka 565-8565, Japan

Received for publication, January 12, 2001, and in revised form, February 23, 2001


    ABSTRACT
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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES

The Na+/H+ exchangers (NHEs) comprise a family of transporters that catalyze cell functions such as regulation of the pH and volume of a cell and epithelial absorption of Na+ and bicarbonate. Ubiquitous calcineurin B homologous protein (CHP or p22) is co-localized and co-immunoprecipitated with expressed NHE1, NHE2, or NHE3 independently of its myristoylation and Ca2+ binding, and its binding site was identified as the juxtamembrane region within the carboxyl-terminal cytoplasmic domain of exchangers. CHP binding-defective mutations of NHE1-3 or CHP depletion by injection of the competitive CHP-binding region of NHE1 into Xenopus oocytes resulted in a dramatic reduction (>90%) in the Na+/H+ exchange activity. The data suggest that CHP serves as an essential cofactor, which supports the physiological activity of NHE family members.


    INTRODUCTION
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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES

The Na+/H+ exchanger (NHE)1 is an electroneutral plasma membrane transporter that catalyzes H+-extrusion coupled to Na+-influx (1, 2). Six identified NHE isoforms exhibit different tissue expression patterns (1-4): NHE1, in all tissues; NHE2-4, mostly in epithelial cells; NHE5, in brain; and NHE6, in mitochondria. These isoforms seem to have distinctive properties despite their overall structural similarity. For example, NHE1 and NHE3, which have been the most intensively studied isoforms, are involved in regulation of intracellular pH and cell volume in all cell types and Na+ and bicarbonate absorption in the epithelial cells, respectively. These two isoforms exhibit very different modes of regulation by many physiological factors as well as a large difference in the sensitivity to inhibitors such as amiloride derivatives (1, 2). Furthermore, targeted gene disruption of NHE1, NHE2, or NHE3 produces remarkably different phenotypes in mice; epilepsy and seizure for NHE1, reduced acid secretion in stomach for NHE2, and reduced salt absorption in kidney and low blood pressure for NHE3, respectively (5-7). The functional diversity may suggest that NHE isoforms have fundamental differences in the regulatory mechanism.

All NHE molecules comprise two major domains, amino-terminal transmembrane (~500 amino acids) and carboxyl-terminal cytoplasmic domains (~300 amino acids). The latter has been suggested to function as a regulatory domain involving multiple accessory factors (1, 2). For example, calmodulin (8, 9) and the NHE3 regulatory factor (10) have been suggested to regulate NHE1 and NHE3 by interacting with their cytoplasmic domains, respectively. Five years ago, Lin and Barber (11) identified a novel Ca2+-binding protein CHP that interacts with NHE1 and exerts regulatory influences on its activity. CHP is ubiquitously expressed and homologous to the calcineurin B subunit (11). This same protein has been identified independently as a factor (called p22) required for the vesicular transport of proteins (12). More recently, this protein has been reported to inhibit the calcineurin phosphatase activity (13) or to associate with microtubules (14). Lin and Barber (11) reported that overexpression of CHP with a tag for detection in CCL39 fibroblastic cells inhibits the activation of NHE1 induced by serum or small G proteins and suggested that CHP may be dissociated from the exchanger upon mitogenic stimulation (11). However, the role of endogenous CHP in NHE1 has not yet been clarified. Our preliminary experiment showed that CCL39 cells express a significant amount of endogenous CHP that may be sufficient to form a complex with endogenous NHE1 at a 1:1 molar ratio. Therefore, the interpretation of the reported effect of CHP overexpression in NHE1 function (11) may not be straightforward. Clearly, to assign the definitive role to CHP, it is necessary to compare the exchanger functions in the presence or absence of bound CHP.

In this study, we have characterized the role of endogenous CHP in the functions of NHE1 and other NHE isoforms not determined previously. We show here that CHP is a key molecule that supports the activities of multiple exchanger isoforms. This is the first study to show that different exchangers require physical interaction with a common protein to express physiological activity.

    MATERIALS AND METHODS
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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES

Cell Culture and cDNA Transfection-- The exchanger-deficient PS120 cells (15) were cultured in Dulbecco's modified Eagle's medium (Life Technologies, Inc.) supplemented with 7.5% fetal calf serum at 37 °C in an atmosphere of 95% air and 5% CO2. Plasmids were transfected into PS120 cells (5 × 105 cells/10-cm dish) by means of the calcium-phosphate co-precipitation technique. Cell populations that stably express NHE variants were selected by means of the repetitive H+-killing selection procedure (16). For stable expression of CHP·GFP fusion protein variants, single clones expressing proteins were isolated using GFP fluorescence as a marker after selection with G418.

Construction of Expression Vectors for NHE and CHP Variants-- Plasmids carrying cDNAs for human NHE1, rat NHE2, and rat NHE3, and their mutants were all cloned into the mammalian expression vector pECE. All these constructs were produced by means of the polymerase chain reaction (PCR)-based strategy (16). The cDNA for CHP was obtained from human blood by reverse transcriptase-PCR based on the reported sequence (11). CHP·GFP fusion protein plasmids were constructed using vector pEGFP-N1 (Clontech) by means of the PCR-based method. Inserted DNA fragments containing mutations were confirmed by sequencing plasmids with a DNA sequencer model 377 (ABI) to ensure the fidelity of construction.

Expression and Purification of NHE and CHP Fusion Proteins-- MBP or GST fusion protein plasmids were constructed using vector pMAL-c (New England Biolabs) or pGEX-2TK (Amersham Pharmacia Biotech) by means of the PCR-based method. Proteins were purified according to the manufacturer's protocol. For production of recombinant CHP, the full CHP sequence with 6 His residues was cloned into bacterial expression vector pET11 (Stratagene). This plasmid was incorporated into Escherichia coli cells (BL21) either alone or together with pBB131 carrying cDNA for yeast N-myristoyltransferase (kindly provided by Dr. Gordon, Washington University). After protein induction, the transformed bacteria were sonicated and centrifuged. The pellet was suspended in the lysis buffer containing M guanidine hydrochloride. CHP proteins were purified on a ProBondTM resin column (Invitrogen), according to the manufacturer's protocol. Proteins were renatured by dialysis overnight against 150 mM NaCl and 10 mM Hepes, Tris, pH 7.4. His-tagged NHE1 proteins were also purified from E. coli in a similar way.

Immunoprecipitation, Immunoblotting, and Surface Labeling with NHS-Biotin-- Polyclonal anti-NHE1 was previously described (8). Polyclonal anti-NHE3 or anti-CHP antibodies were produced by immunizing rabbits with the MBP·NHE3-(470-831) or GST·CHP-(1-195) fusion proteins. Immunoprecipitation and immunoblotting were performed essentially as described previously (8, 16). Briefly, cells were solubilized with 1% Triton X-100 in 20 mM Hepes, Tris, pH 7.4, 150 mM NaCl, and protease inhibitors, and the cell lysate was incubated with the antibodies indicated and protein A-Sepharose. Precipitated materials were separated on 7.5 or 12% polyacrylamide gels and transferred to Immobilon membranes (Millipore Japan). After blocking, incubation with antibodies, and washing, protein signals were visualized using enhanced chemiluminescence (Amersham Pharmacia Biotech).

For surface labeling of NHE1 or NHE3, cells were incubated with 1 mM NHS-biotin for 30 min at room temperature, solubilized with the lysis buffer, and then incubated for 1 h with streptavidin-agarose beads as described (17). After washing, the proteins were released from the beads by boiling in 3% SDS buffer and then subjected to immunoblot analysis with the antibodies indicated.

Far-Western Analysis and Pull-down Assay-- For far-Western analysis, blots for various MBP·NHE1 fusion proteins were incubated for 1 h with 100 µg/ml GST·CHP proteins, and then bound GST·CHP fusion proteins were visualized by immunoblotting with anti-GST antibody (Amersham Pharmacia Biotech). For the pull-down assay for CHP proteins, non-myristoylated or myristoylated CHP (150 µg each) was incubated for 30 min at 4 °C with 30 µl of amylose resin pretreated with the MBP·NHE1-(503-815) fusion protein (200 µg) in buffer (in mM: NaCl, 150; Hepes/Tris, pH 7.4, 10; and CaCl2, 1; or EDTA, 1). After washing, the proteins were eluted from resin with 50 mM maltose, electrophoresed, and then visualized by Coomassie Brilliant Blue staining.

Immunostaining-- Cells were fixed and permeabilized with cold methanol and incubated with anti-NHE1 antibody, followed by incubation with rhodamine-labeled second antibody as described (18). For observation of GFP fluorescence, cells stably expressing CHP·GFP proteins were placed in serum-free Dulbecco's modified Eagle's medium without phenol red. Images were taken under a fluorescent microscope equipped with a CoolSNAP imaging system (RS Photometrics).

Crude Membrane Preparation from Xenopus Oocytes-- Xenopus oocytes were stripped and defolliculated enzymatically with 1 mg/ml collagenase in a Ca2+-free ND96 solution (in mM: NaCl, 96; KCl, 2; MgCl2, 1; and Hepes/NaOH, pH 7.5, 5) for 30 min at room temperature. For membrane preparation, 100 oocytes injected with each protein were homogenized for 60 s with Physcotron (Nition Co.) in a medium (in mM: NaCl, 20; Mg-acetate, 10; phenylmethylsulfonyl fluoride, 1; and Tris-HCl, pH 7.6, 20), and then subjected to discontinuous sucrose gradient centrifugation. After centrifugation for 30 min at 15,000 g, the 20-50% interface was collected as the crude membrane fraction. Membranes were solubilized with lysis buffer (in mM: KCl, 100; MgCl2, 5; CaCl2, 1; phenylmethylsulfonyl fluoride, 1; and Tris-HCl, pH 8.2, 100) containing 1% Triton X-100, 0.5% SDS, and 1% sodium deoxycholate and was then subjected to immunoprecipitation.

Measurement of 22Na+ Uptake-- The rates of ethylisopropyl-amiloride (EIPA)-sensitive 22Na+ uptake by PS120 cells expressing NHE variants were measured using cells pHi-clamped at 5.6 by the K+/nigericin method (19). For measurement of 22Na+ uptake by oocytes, cells were preincubated for 1 h in NH4Cl medium (in mM: NH4Cl, 80; CaCl2,1; MgCl2, 1; and Hepes/Tris, pH 7.4, 10), washed twice with choline-Cl medium (in mM: choline-Cl, 80; CaCl2, 1; MgCl2, 1; and Hepes/Tris, pH 7.4, 10) and then incubated for 15 min in the same medium additionally containing 1 mM 22NaCl (370 kBq/ml), 1 mM ouabain, and 0 or 0.1 mM EIPA. Oocytes were washed four times with ice-cold, nonradioactive choline-Cl medium, and then 22Na radioactivity was counted.

    RESULTS AND DISCUSSION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES

Identification and Characterization of the CHP-binding Domain in NHEs-- CHP is a myristoylated protein with four Ca2+-binding motifs (EF-hands) (Refs. 11, 12; Fig. 1a). CHP is expressed in virtually all cells (11) including the exchanger-deficient PS120 cells used in our expression study (Fig. 1b). Co-immunoprecipitation studies involving PS120 cells stably expressing NHE1 revealed that CHP is tightly associated with NHE1 (Fig. 1b). To identify the CHP-binding domain in NHE1, we produced a series of MBP fusion proteins containing various regions of the carboxyl-terminal cytoplasmic domain (amino acids 503-815) of NHE1 (see Fig. 1a) and examined their interaction with GST·CHP fusion proteins by far-Western analysis (Fig. 1, c and d). The analysis revealed that amino acids 515-530 of NHE1 are required for CHP-binding (Fig. 1, c and d), which was further confirmed by co-immunoprecipitation experiments involving cells expressing several deletion mutants of NHE1 (Fig. 1e). The identified CHP-binding domain is different from the previously reported one (amino acids 567-635; Ref. 11). The sequence of the CHP-binding domain was found to be well conserved among mammalian NHE isoforms NHE1-5 (see Fig. 2a), suggesting that all these isoforms may interact with CHP. In fact, CHP bound to NHE3 (see Fig. 2d). Furthermore, we produced two chimeric exchangers, NHE1(N2) and NHE1(N4), in which amino acids 503-600 of NHE1 were replaced by the corresponding region of NHE2 or NHE4, respectively (Fig. 1c). CHP also bound to these exchangers (Fig. 1f). Thus, NHE1-4 all contain CHP-binding sites in the juxtamembrane region of the carboxyl-terminal cytoplasmic domain.


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Fig. 1.   Interaction of NHE with CHP. a, models of the structures of NHE1 and CHP. NHE1 comprises two major domains, amino-terminal transmembrane (amino acids 1-500) and carboxyl-terminal cytoplasmic domains (amino acids 500-815). In this figure, the secondary structure model of NHE1 is shown as reported previously (17). b, co-immunoprecipitation of CHP and NHE1 proteins performed with specific antibodies. Lysates of exchanger-deficient PS120 cells or NHE1 transfectants were subjected to immunoprecipitation (IP) followed by immunoblot analysis (IB) with the antibodies indicated in the figure as described under "Materials and Methods." c, constructs for MBP fusion proteins containing various regions of the NHE1 cytoplasmic domain and their CHP binding ability determined as in b by far-Western assaying with GST·CHP fusion protein (+++, strong; +, weak; -, no binding). The last two constructs represent chimeric exchangers in which amino acids 503-815 of NHE1 were replaced by corresponding regions of NHE2 or NHE4. d, far-Western analysis was carried out as described under "Materials and Methods." The left panel shows protein inputs (10 µg). e and f, cells expressing various NHE variants were subjected to co-immunoprecipitation experiments with the antibodies indicated in the figure. g, pull-down assay for non-myristoylated or myristoylated CHP. Proteins were eluted through amylose resin immobilized with MBP·NHE1 and visualized by Coomassie Brilliant Blue staining as described under "Materials and Methods." Control, no pretreatment with the MBP·NHE1 protein.


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Fig. 2.   Properties of NHE mutants. a, amino acid sequence alignment of the CHP-binding domain of NHEs and the mutant constructs. The CHP binding domains of mammalian NHE1-5, Xenopus NHE, and the mutant constructs were aligned. Hydrophobic amino acids are underlined. The helical wheel diagram of the CHP-binding domain is shown for amino acids 518-535 of NHE1. b, expression level of various NHE variants. Lysates (20 µg) of cells expressing the mutant exchangers shown in a were analyzed by immunoblotting with anti-NHE1 (left) or anti-NHE3 (right) antibody. c, surface labeling of the NHE1 or NHE3 protein was carried out using NHS-biotin as described under "Materials and Methods." d, lysates of cells expressing various NHE variants were subjected to co-immunoprecipitation experiments with anti-CHP antibody followed by immunoblotting with ant-NHE1 or anti-NHE3 antibody. e and f, the extents of surface labeling of NHE1 variant proteins with NHS-biotin as in c and the amounts of them recovered on immunoprecipitation with anti-CHP antibody as in d, normalized as to the expression level of the high molecular weight NHE1 protein. The data represent means ± S.D. (n = 3), relative to the values for wild-type NHE1 (WT). g, the rates of EIPA-sensitive 22Na+ uptake by NHE1 variants were measured using cells pHi-clamped at 5.6. Inset, data for NHE2 and NHE3 and their mutants. Data are means ± S.D. (n = 3).

We have produced His-tagged recombinant CHP proteins with and without a myristoyl moiety using an E. coli expression system. Both CHP proteins bound to NHE1 in the absence or presence of Ca2+, as revealed in the pull-down assay with MBP·NHE1 fusion proteins (Fig. 1g). Thus, myristoylation and Ca2+ binding in CHP are not required for the interaction of CHP with NHE1. It is noteworthy that the Ca2+-bound form of myristoyl-CHP migrated faster (Fig. 1g). Thus, the Ca2+-induced conformational change in CHP seems to require its myristoylation as in the case of other Ca2+-binding myristoylated proteins (20).

Effects of Mutation within CHP-binding Domains of NHEs on Surface Expression, CHP-binding, and the Exchange Activity-- The CHP-binding domain of NHE1 is predicted to form a conserved alpha -helix like the calcineurin B-binding domain within the calcineurin A subunit (Refs. 21, 22; Fig. 2a). A helical wheel diagram for amino acids 518-535 of NHE1 revealed a cluster of hydrophobic residues (Fig. 2a). Based on the previous finding that a hydrophobic interaction is important for the interaction between two calcineurin subunits (21, 22), we produced several mutant plasmids carrying amino acid substitutions of hydrophobic to hydrophilic residues (Gln or Arg) as well as other substitutions (to Ala) (see Fig. 2a for the naming of mutants). When transfected into cells, these NHE1 variants were expressed in high and low molecular weight forms that correspond to glycosylated mature and immature NHE1 proteins, respectively (Ref. 23; Fig. 2b, not shown for some mutants). The mature proteins from all these constructs were labeled efficiently with membrane-impermeable NHS-biotin applied from the outside (Fig. 2c), indicating the surface expression of these proteins. NHE1 mutants 4Q, 4R, 6Q, and 2Q/4R were not co-immunoprecipitated with CHP (Fig. 2d). These findings suggest that CHP is not required for the surface expression of NHE1.

Intriguingly, these mutations 4Q, 4R, 6Q, and 2Q/4R dramatically reduced the exchange activity, as measured as EIPA-sensitive 22Na+ uptake at an acidic intracellular pH of 5.6, to <10% of the level in the wild-type (Fig. 2g). A similar reduction in activity also occurred for deletion mutants Delta 510-530 and Delta 515-530 (not shown). As summarized in Fig. 2, f and g, the exchange activity changed in parallel with the CHP-binding ability for various NHE1 mutants, despite the fact that nearly the same amounts of proteins were expressed in the surface (Fig. 2e). These data suggest that CHP binding is required for optimal activity of NHE1. We could not further analyze regulatory or kinetic properties of CHP binding-defective mutant exchangers (4Q, 4R, 6Q, and 2Q/4R) because of their low activity (Vmax). However, cells expressing a mutant (518Q/522Q) with low affinity for CHP but with modest activity show similar properties to the wild-type NHE1, i.e. an intracellular pH dependence of 22Na+ uptake with a pK of ~6.6 and cytoplasmic alkalinization by 0.1 ~ 0.2 pH unit in response to alpha -thrombin, platelet-derived growth factor, phorbol ester, and hyperosmotic stress (data not shown). These properties probably reflect those of the CHP-bound but not CHP-unbound mutant exchanger because only the former exhibits measurable high activity as shown in oocyte experiments described below. Similar to NHE1 mutants, NHE2-3R or NHE3-4R mutants (see Fig. 2a) did not interact with CHP (see Figs. 2d and 4, h-k). In addition, these CHP binding-defective mutants of NHE2 and NHE3 exhibited markedly reduced exchange activity (Fig. 2g, inset).

Injection of CHP-binding Region of NHE1 Dramatically Reduces the Activity of Xenopus Oocyte Exchanger-- The above data strongly suggest that CHP binding is required for the exchange activity of NHE1-3. To examine this further, we used a Xenopus oocytes expression system. Oocytes are known to have an endogenous NHE, whose amino acid sequence is similar to that of human NHE1 (overall homology, 78%; Refs. 24, 25). CHP-binding domains were well conserved in the two species (see Fig. 2a). Oocytes also have an endogenous CHP detectable on immunoblotting with anti-human CHP antibody (Fig. 3a). We produced His-tagged human NHE1 proteins (amino acids 503-600) containing the wild-type sequence or CHP binding-defective mutations (Fig. 3b). Injection of the His-tagged wild-type protein into oocytes, but not that of mutant proteins, resulted in the disappearance of Xenopus NHE1 from the CHP immunoprecipitates (Fig. 3c, middle). Furthermore, anti-CHP antibody co-precipitated the His-tagged wild-type protein with endogenous CHP, but not mutant proteins (Fig. 3c, bottom). These data indicate that the injected human CHP-binding protein depletes bound CHP from Xenopus NHE. Importantly, we found that injection of the His-tagged wild-type protein abolished the endogenous exchange activity in oocytes almost completely (Fig. 3d). A relatively long time (>= 3 h) was required for inhibition of the exchange activity after injection of proteins (Fig. 3e), suggesting that CHP is slowly released from Xenopus NHE. On the other hand, the exchange activity of human NHE1 expressed in oocytes was significantly increased when human CHP was co-expressed (Fig. 3f). These data also strongly suggest that CHP is required for high exchange activity.


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Fig. 3.   Analysis of NHE function in Xenopus oocytes. a, immunoblot for oocyte proteins (50 µg) with anti-CHP antibody. b, purified recombinant His-tagged proteins (10 µg) containing the wild-type sequence (amino acids 503-600 of NHE1) or CHP binding-defective mutations were visualized by Coomassie Brilliant Blue staining. c, His-tagged proteins (20 ng/50 nl) were injected into oocytes, from which crude membranes were prepared 8 h later. Using solubilized crude membranes, co-immunoprecipitation experiments were performed with the antibodies indicated (anti-His antibody from Roche Molecular Biochemicals. d, eight hours after the injection of His-tagged proteins, 22Na+ uptake into oocytes containing endogenous NHE was measured in the presence or absence of 0.1 mM EIPA as described under "Materials and Methods." The 22Na+ uptake was 40-50 pmol/oocyte/15 min in the presence of EIPA. e, time course of EIPA-sensitive 22Na+ uptake after injection of the His-tagged wild-type protein. f, three days after injection of cRNAs (50 ng) into oocytes, EIPA-sensitive 22Na+ uptake was measured. Data are means ± S.D. of the values for at least 10 oocytes.

Subcellular Localization of GFP-tagged CHP Proteins-- CHP fusion protein conjugated with green fluorescent protein (CHP·GFP) was expressed uniformly in the cytosol of PS120 cells (Fig. 4c). This fusion protein became partly localized in the cell surface when exogenous NHE1 was co-expressed (Fig. 4d). However, CHP·GFP was not localized in the surface membrane when NHE1 mutant 4Q was expressed in the membrane (Fig. 4, b and e). Therefore, NHE1 seems to be the principal target for CHP in the membrane. We stably overexpressed two GFP-conjugated CHP mutant proteins (CHP2A·GFP and CHPEF3·GFP) that lack the ability to be myristoylated or to bind Ca2+ (12). Consistent with the in vitro binding data (see Fig. 1g), these proteins were partly localized in the surface membrane together with the NHE1 protein (Fig. 4, f and g), suggesting that they might have replaced endogenous CHP. Because expression of these proteins did not inhibit 22Na+ uptake activity (Fig. 4l), it appears that neither myristoylation nor Ca2+ binding is essential for exchange activity. CHP·GFP was also partly localized in the surface membrane when it was co-expressed with NHE2 or NHE3, although a significant amount of CHP·GFP still remained in the cytosol or an intracellular compartment (Fig. 4, h and j). However, such surface expression was abolished by mutations (NHE2-3R and NHE3-4R) introduced into these NHE isoforms (Fig. 4, i and k), indicating that CHP binding was lost in these mutants.


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Fig. 4.   Subcellular localization of CHP·GFP proteins and exchange activity of various stable transfectants. PS120 cells expressing various NHE variants and/or CHP·GFP proteins were seeded onto 60-mm dishes. a and b, immunostaining with anti-NHE1 antibody. c-k, fluorescent images of cells stably expressing CHP·GFP proteins. Gly-2 of CHP was replaced by Ala in CHP2A·GFP, whereas Asp-133/Glu-134 in the third EF-hand motif were replaced by Ala/Met, respectively, in CHPEF3·GFP. Scale bar, 20 µm. l, EIPA-sensitive 22Na+ uptake activity was measured using cells stably expressing NHE1 and various CHP·GFP fusion proteins. Data are means ± S.D. (n = 3).

It has generally been considered that a single polypeptide for each NHE isoform is sufficient for normal exchange activity. However, the present data provide evidence that NHE1 requires an extra cofactor protein, CHP, for it to express physiological activity. Our data also suggest the same role of CHP for NHE2-4. A potential CHP-binding motif also exists in NHE5 (Ref. 3; see Fig. 2a), but not in mitochondrial NHE6 (4). Based on these findings, we propose that CHP functions as an integral cofactor common to plasma membrane-type Na+/H+ exchangers.

Na+/H+ exchange presumably occurs through the transmembrane domain of an exchanger. Cells expressing a NHE1 mutant (Delta 515) with the complete carboxyl-terminal cytoplasmic domain (amino acids 516-815) deleted still retain low exchange activity that can be detected by expressing a high copy number of a mutant molecule (16). This is consistent with the present finding that mutants lacking the CHP-binding site are able to exhibit low exchange activity (5-10% of the wild-type level). We previously showed that the exchange activity at physiological pHi is markedly decreased by deletion of different regions in the amino terminus (subdomain I, amino acids 515-595) of the NHE1 cytoplasmic domain (19). These data suggest that subdomain I is essential for normal exchange activity. This domain with bound CHP would therefore function as a key structure that interacts with the transmembrane domain (see Fig. 1a), thereby permitting the ion translocation pathway to maintain its physiologically relevant active conformation. More detailed structural information including the crystal structure of the CHP·NHE complex is necessary to reveal how CHP is involved in this important task of subdomain I.

Subdomain I contains the CHP-binding region (amino acids 515-530) as well as two potential phosphatidylinositol 4,5-bisphosphate (PIP2) binding motifs (amino acids 509-516 and 552-560 for the human NHE1 sequence) identified by Aharonovitz et al. (26) who provided evidence that a reduction of cell PIP2 causes marked inhibition of the exchange activity of NHE1. Their study, however, provided little information regarding whether or not the effect of PIP2 is mediated by the above binding motifs in situ. In this study, we found that the injected His-tagged wild-type NHE1 protein (amino acids 503-600) almost completely abolished the exchange activity in oocytes, whereas mutant protein 4R lacking the ability to bind CHP did not (Fig. 3d). This finding seems to argue against the view that the function of CHP is directly related to PIP2, because the injected wild-type and mutant 4R proteins would not be much different in their PIP2 binding (see Fig. 2a).

We observed that the surface localization of CHP·GFP in NHE1 transfectants was not affected significantly by ionomycin, ATP depletion, phorbol ester, serum, or other growth factors (not shown), which is inconsistent with the previous proposal (11) that CHP may be dissociated from NHE1 upon growth factor activation. Whether or not the bound CHP is involved in acute regulation of the exchanger is not clear at present. Although CHP interacts with multiple NHE isoforms, it is still possible that the activity of the exchanger is regulated through post-translational modifications of the associated CHP in response to extracellular stimuli. Indeed, myristoylated CHP alters its conformation in a Ca2+-dependent manner (see Fig. 1g), similar to some other Ca2+-myristoyl switch proteins (20). It is possible that such a conformational change of bound CHP alters the structure of subdomain I and regulates the transport activity.

In conclusion, the present study revealed that multiple exchangers require physical interaction with a common protein, CHP for exchange activity. This function does not appear to require Ca2+ binding and myristoylation in CHP. To our knowledge, this is the first finding to show that a Ca2+-binding protein supports the activity as an integral component of the secondary active transporter.

    ACKNOWLEDGEMENT

We thank Dr. T. Y. Nakamura for technical assistance in the Xenopus oocyte experiments.

    FOOTNOTES

* This work was supported by Grants-in-aid for Scientific Research, 09680642 and 10470013, from the Ministry of Education, Science, and Culture of Japan, and the Uehara Memorial Foundation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed. Tel.: 81-6-6833-5012; Fax: 81-6-6872-7485; E-mail: wak@ri.ncvc.go.jp.

Published, JBC Papers in Press, February 28, 2001, DOI 10.1074/jbc.M100296200

    ABBREVIATIONS

The abbreviations used are: NHE, Na+/H+ exchanger; CHP, calcineurin B homologous protein; GFP, green fluorescent protein; MBP, maltose-binding protein; GST, glutathione S-transferase; PCR, polymerase chain reaction; NHS-biotin, N-hydroxysuccinimido-biotin; EIPA, ethylisopropyl-amiloride.

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES

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