Interaction of 11-cis-Retinol Dehydrogenase with the Chromophore of Retinal G Protein-coupled Receptor Opsin*

Pu ChenDagger , Terry D. Lee§, and Henry K. W. Fong

From the Dagger  Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry and  Departments of Ophthalmology and Microbiology, University of Southern California School of Medicine and Doheny Eye Institute, Los Angeles, California 90033 and § Beckman Research Institute of the City of Hope, Duarte, California 91010

Received for publication, November 17, 2000, and in revised form, March 26, 2001


    ABSTRACT
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
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DISCUSSION
REFERENCES

Vertebrate opsins in both photoreceptors and the retinal pigment epithelium (RPE) have fundamental roles in the visual process. The visual pigments in photoreceptors are bound to 11-cis-retinal and are responsible for the initiation of visual excitation. Retinochrome-like opsins in the RPE are bound to all-trans-retinal and play an important role in chromophore metabolism. The retinal G protein-coupled receptor (RGR) of the RPE and Müller cells is an abundant opsin that generates 11-cis-retinal by stereospecific photoisomerization of its bound all-trans-retinal chromophore. We have analyzed a 32-kDa protein (p32) that co-purifies with bovine RGR from RPE microsomes. The co-purified p32 was identified by mass spectrometric analysis as 11-cis-retinol dehydrogenase (cRDH), and enzymatic assays have confirmed the isolation of an active cRDH. The co-purified cRDH showed marked substrate preference to 11-cis-retinal and preferred NADH rather than NADPH as the cofactor in reduction reactions. cRDH did not react with endogenous all-trans-retinal bound to RGR but reacted specifically with 11-cis-retinal that was generated by photoisomerization after irradiation of RGR. The reduction of 11-cis-retinal to 11-cis-retinol by cRDH enhanced the net photoisomerization of all-trans-retinal bound to RGR. These results indicate that cRDH is involved in the processing of 11-cis-retinal after irradiation of RGR opsin and suggest that cRDH has a novel role in the visual cycle.


    INTRODUCTION
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
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DISCUSSION
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The continual synthesis of rhodopsin by recombination of its apoprotein with the chromophore, 11-cis-retinal, is an essential process that maintains visual excitation (1, 2). It has long been known that formation of 11-cis-retinal for regeneration of rhodopsin is dependent on retinoid metabolic reactions in the retinal pigment epithelium (RPE),1 where the majority of enzymes of the visual cycle are located (reviewed in Ref. 3). In the current model of the visual cycle, all-trans-retinal from bleached rhodopsin is reduced to all-trans-retinol by an all-trans-retinol-specific dehydrogenase (tRDH) located in photoreceptor outer segments (4-6). The all-trans-retinol is then delivered to the RPE, where it is converted by the lecithin:retinol acyltransferase to all-trans-retinyl ester (7, 8). A key step in the visual cycle is performed by an isomerohydrolase that catalyzes the formation of 11-cis-retinol from all-trans-retinyl ester (9-10). Alternatively, the isomerization of all-trans-retinol to 11-cis-retinol is achieved via an intermediate with an anhydro-like carbocation structure (11). An 11-cis-retinol-specific dehydrogenase (cRDH) is able to oxidize 11-cis-retinol to 11-cis-retinal (12-14), which is transferred to the outer segments of photoreceptors for recombination with opsin to form rhodopsin. Under light-adapted conditions, the rate of synthesis of 11-cis-retinal must be sufficient for regeneration of steady-state levels of visual pigments (15, 16).

Besides isomerohydrolase, another type of isomerase in the RPE may include the retinochrome-like visual pigment homologues peropsin or the RPE retinal G protein-coupled receptor (RGR) opsin (17, 18). In contrast to the visual pigments, RGR is bound in the dark to endogenous all-trans-retinal and is localized to intracellular membranes in RPE and Müller cells (19, 20). Upon illumination, all-trans-retinal bound to RGR is photoisomerized stereospecifically to the 11-cis isomer (20). These results provide evidence that RGR may function to generate 11-cis-retinal in vivo and participate in a light-dependent photic visual cycle.

A proposed mechanism of RGR function is that 11-cis-retinal dissociates from irradiated RGR and directly enters the pathway for regeneration of rhodopsin under photic conditions. A central hypothesis of the photoisomerase model is that exchange of the chromophore bound to RGR occurs and involves distinct geometrical isomers. Retinal must uncouple and bind anew to RGR through a stereospecific cycle that is driven by light energy. The observation that microsomal RGR can be labeled with 3H-labeled all-trans-retinal (21) and that ~50% of RGR isolated from RPE is in the form of the apoprotein (22) suggests that the binding of retinal is reversible and that the process of binding and dissociation of the chromophore occurs in vivo.

One of the factors that may control Schiff base hydrolysis and dissociation of 11-cis-retinal from RGR in vivo is specific protein interactions. We have observed that when RGR is isolated in digitonin solution from bovine RPE microsomes by immunoaffinity chromatography, it co-purifies consistently with a 32-34-kDa protein (p32) (22). It is possible that the co-purified protein forms a physical complex or associates functionally with RGR. Hence, the identification of the co-purified p32 protein may provide important insights into the mechanism of RGR function.

In this paper, we report the analysis and characterization of co-purified p32, which has been identified as 11-cis-retinol dehydrogenase of the RPE. We demonstrate enzymatic activity of the co-purified cRDH and discuss its potential role in RGR function.

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Materials-- Digitonin was obtained from Calbiochem-Novabiochem. Hydroxylamine and all-trans-retinal were purchased from Sigma. 11-cis-Retinal was provided by Dr. Rosalie Crouch (Medical University of South Carolina, Charleston, SC). An 11-cis-retinol standard was prepared by reduction of 11-cis-retinal in the presence of NaBH4 and was purified by high performance liquid chromatography (HPLC) as described previously (23). Organic solvents were HPLC grade. Dichloromethane and hexane were obtained from Fisher. Ether and methanol were from J. T. Baker Inc. Dioxane was from Burdick & Jackson.

Isolation of RGR-- All isolation procedures were performed under dim red light. RPE cells were isolated from fresh bovine eyes, as described previously (22). The cells were washed in ice-cold 0.25 M sucrose, 30 mM sodium phosphate buffer, pH 6.5, and homogenized in a glass Dounce homogenizer. After a low speed centrifugation at 300 × g, the homogenate was centrifuged at 15,000 × g for 20 min at 4 °C. RPE microsomes in the supernatant were sedimented by centrifugation at 150,000 × g for 1 h at 4 °C and stored at -80 °C until later use. For isolation of RGR, the microsomal membranes were extracted three times for 1 h at 4 °C with 1.2% digitonin solution containing 10 mM sodium phosphate, pH 6.5, 150 mM NaCl, and 0.5 mM EDTA. The extracts were centrifuged each time at 100,000 × g for 20 min at 4 °C. The pooled supernatants were incubated overnight at 4 °C with Affi-Gel 10 resin (Bio-Rad) conjugated to monoclonal antibody 2F4, which is directed to the carboxyl terminus of bovine RGR (21). The resin was transferred to a column and washed with 25 bed volumes of wash buffer (0.1% digitonin in 10 mM sodium phosphate, pH 6.5, 150 mM NaCl, and 0.5 mM EDTA). RGR was eluted from the column with 10 times 0.5 bed volumes of wash buffer containing 50 µM bovine RGR carboxyl-terminal peptide (CLSPQRREHSREQ). The eluted fractions were pooled and concentrated ~4-fold using a Centricon 3 concentrator (Amicon, Inc., Beverly, MA). The proteins were analyzed by SDS-polyacrylamide gel electrophoresis and stained with Coomassie Blue. The amount of isolated protein was determined with a densitometer by measurement of relative band intensity and comparison with known amounts of bovine serum albumin protein standard. The amount of RGR used in some experiments was based on a typical yield of ~64 ng/eye isolated from 15-80 eyes. In a modification of the isolation procedure, 20% glycerol was included in all buffers for the isolation of RGR from RPE microsomes, as indicated in Fig. 5.

Mass Spectrometry-- Immunoaffinity-purified RGR was electrophoresed in a 15% SDS-polyacrylamide gel and stained with Coomassie Blue. A 32-kDa protein (p32) was found to co-purify consistently with RGR. The p32 protein band was excised from the gel and used for a trypsin in-gel digestion (24). A portion of the digested peptide mixture was analyzed using a custom-built capillary liquid chromatography system (25) interfaced directly to a Thermo-Finnigan (San Jose, CA) LCQ ion trap mass spectrometer, as described previously (26). Full mass range spectra, high resolution zoom scan spectra, and fragment ion (MS/MS) spectra were collected using the automated, data-dependent acquisition functions of the LCQ data system. The protein identification was made by correlating the collected MS/MS spectra to the OWL protein sequence data base using the Sequest data base search program (27).

11-cis-Retinol Dehydrogenase Assay with Exogenous Substrate-- The reduction activity of cRDH was measured as described previously (12, 28). The thiobarbituric acid method was used to monitor the colored derivative formed by retinal and thiobarbituric acid (29). The immunoaffinity-purified RGR or RPE microsomal proteins were used as the source of cRDH enzyme. For the reduction of exogenous substrate, the substrate solution contained 10 nmol of exogenous retinal isomer, 12% acetone, 1.2% Tween 80, 1 mM NADH or NADPH, and 0.2 M sodium acetate buffer, pH 5.0. Controls were performed by omitting the NADH or NADPH cofactor or by addition of a heat-inactivated protein sample. The reactions were initiated by the addition of 50 µl of substrate solution to 50 µl of protein solution. After incubation at 37 °C, the reactions were terminated by addition of 0.5 ml of ethanol. Subsequently, 0.2 ml of thiourea and 0.2 ml of thiobarbituric acid reagent were added, and the samples were incubated for 30 min at room temperature. The solutions were centrifuged at 14,000 rpm on a tabletop centrifuge (Eppendorf Centrifuge 5415C, Germany) for 1 min. 50 µl of distilled water was added to each supernatant to prevent precipitation. Color development was measured at 530 nm. Ethanol was used as a reagent blank for the color development. The concentration of exogenous retinal isomer was determined by absorbance measurements with a Hitachi U-3000 scanning spectrophotometer. NADH and NADPH were prepared immediately before use in 5 mM Tris-HCl, pH 7.5.

Extraction of the Chromophore of RGR by Hydroxylamine Derivatization-- The retinal chromophore of RGR was extracted under dim red light and analyzed by the method of hydroxylamine derivatization, as described by Groenendijk et al. (30, 31). In a typical extraction procedure, 100-300 µl of purified RGR were mixed with 0.1 volume of 2 M hydroxylamine, pH 6.5, and then 300 µl of methanol and 300 µl of dichloromethane. The sample volume was brought to 900 µl by the addition of 10 mM sodium phosphate buffer, pH 6.5, containing 150 mM NaCl and 0.5 mM EDTA. The extraction with dichloromethane (aqueous buffer/methanol/dichloromethane, 1:1:1 by volume) was performed by vortexing for 30 s. After centrifugation at 14,000 rpm for 1 min, the lower organic phase was removed, and the upper phase was extracted twice more with 300 µl of dichloromethane. The organic layers were pooled and dried under a nitrogen stream. The extracted retinaloximes were then solubilized in 600 µl of hexane, filtered through glass wool, and dried again under nitrogen. The residue was either stored in darkness at -80 °C or analyzed immediately by HPLC.

HPLC Analysis of Retinaloximes-- The isomers of retinaloximes were analyzed by HPLC, as described previously (20). The extracted retinaloximes were dissolved in hexane and applied to a LiChrosorb RT Si60 silica column (4 × 250 mm, 5 µm) (Merck). The HPLC system was equipped with a Beckman model 126 solvent module and model 166 UV-visible detector (Beckman Instruments). The 20-µl samples were injected and the components were resolved in a running solvent consisting of hexane supplemented with 8% diethyl ether and 0.33% ethanol with flow rate of 1 ml/min. The HPLC column was calibrated using all-trans- and 11-cis-retinaloxime standards. Identification of the retinaloxime isomers was based on the retention times of the known retinaloxime products. Absorbance was measured at 360 nm, and the absorbance peaks were analyzed with the Gold Nouveau software (Beckman Instruments). The proportion of each isomer in the loading sample was determined from the total peak areas of both its syn- and anti-retinaloxime and was based on the following extinction coefficients (epsilon 360, in hexane): all-trans syn = 54,900, all-trans anti = 51,600, 11-cis syn = 35,000, 11-cis anti = 29,600, 13-cis syn = 49,000, and 13-cis anti = 52,100 (31, 32).

HPLC Analysis of 11-cis-Retinol-- 11-cis-Retinol was co-extracted with retinaloximes and analyzed by HPLC, as described above. In some experiments, the retinoids were separated on a Resolve silica column (3.9 × 150 mm, 5 µm) (Waters Corp., Milford, MA) using a Waters 2690 HPLC module. The samples were resolved in a running solvent consisting of hexane supplemented with 8% diethyl ether and 0.33% ethanol. Retinaloximes and 11-cis-retinol were measured simultaneously with a Waters 2487 dual wavelength absorbance detector at 360 and 320 nm, respectively; sensitivity was ~6-fold higher than that of our Beckman system. The absorbance peaks were analyzed with the Millennium 32 Chromatography Manager software, version 3.20 (Waters Corp.). Identification of 11-cis-retinol was based on the retention time of a purified standard. The 11-cis-retinol standard (lambda max = 318 nm) was prepared from known 11-cis-retinal, as described previously (23). The HPLC system was calibrated with 8.8-0.88 pmol of 11-cis-retinol and 9.1-0.91 pmol of syn-all-trans-retinaloxime standards.

Irradiation of RGR and 11-cis-Retinol Dehydrogenase Activity toward the Endogenous Chromophore-- RGR was irradiated with 470-nm monochromatic light using an Oriel light source (Oriel Corp., Stratford, CT) equipped with a 150-W xenon arc lamp. Monochromatic light beams at 470 nm were formed by passing the light through a 470-nm interference filter (Oriel #53845) and 455-nm long pass filter (Oriel #51284). Nonirradiated control samples were held under dim red light, but otherwise were treated identically. The samples contained immunoaffinity-purified RGR in 0.1% digitonin, 10 mM sodium phosphate, pH 6.5, 150 mM NaCl, 0.5 mM EDTA, and 20% glycerol and were incubated in the presence of 3 mM NADH cofactor or in buffer only as indicated under "Results." NADH was freshly prepared in 5 mM Tris-HCl buffer, pH 7.5. The final reaction volume (100 µl) was adjusted by the addition of 10 mM sodium phosphate, pH 6.5, 150 mM NaCl, and 0.5 mM EDTA. Incubations were performed for the desired time at 37 °C in a quartz cuvette positioned 60 cm from the light source. After delivery of the intended amount of light, the mixture was taken out of the water bath, and 0.2 M hydroxylamine was added to stop the reaction. The retinal chromophore was extracted by hydroxylamine derivatization and analyzed by HPLC, as described previously. The incubation of RGR at 37 °C rather than room temperature did not affect the distribution of retinal isomers from irradiated RGR.

    RESULTS
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
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DISCUSSION
REFERENCES

Isolation of RGR and Identification of Co-purified 32-kDa Protein-- RPE microsomal membrane proteins were extracted in 1.2% digitonin solution at pH 6.5, and solubilized RGR was purified by immunoaffinity chromatography under dim red light. Under these conditions, a 32-kDa protein (p32) was found to co-purify reproducibly with RGR (Fig. 1). The p32 protein band was readily detectable in SDS-polyacrylamide gels by staining with Coomassie Blue. It was not recognized on Western blots by the antibody that was coupled to the immunoaffinity resin, even when the immunoblots were overdeveloped (results not shown). The relative amount of p32 that was co-purified with RGR was significantly increased by the inclusion of glycerol in the isolation procedure (Fig. 1A, lane 3). When the antibody binding sites of the immunoaffinity column were blocked by prewashing with excess peptide, neither RGR nor p32 was eluted from the column (Fig. 1B), and p32 did not appear isolatable as a nonspecifically adsorbed protein. The p32 band was excised from a SDS-polyacrylamide gel and analyzed by liquid chromatography MS/MS. When the resulting MS/MS spectra were correlated to the data base of known protein sequences, a number of them gave positive matches to 11-cis-retinol dehydrogenase, a known critical enzyme of the visual cycle (Fig. 2).


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Fig. 1.   Co-purification of RGR and p32 from bovine RPE. RGR was isolated from digitonin extracts of RPE microsomal proteins by an immunoaffinity procedure. The isolated proteins were electrophoresed on SDS-polyacrylamide gels and detected by Coomassie Blue (A) or silver staining (B). A, a 10-kDa protein ladder (10-200 kDa) was used as a molecular weight standard (lane 1). The isolation of RGR was performed in the absence (lane 2) or in the presence (lane 3) of 20% glycerol. RGR isolated from 6.7 and 4.3 eyes was loaded in lanes 2 and 3, respectively. B, RGR was isolated without glycerol (lane 1). The isolation procedure was also carried out with an immunoaffinity column that was first prewashed with one bed volume of 600 µg/ml peptide to block all antibody binding sites. All other purification steps were unchanged. In this control, the 32-kDa protein was not eluted from the column (lane 2). The arrows indicate RGR and the co-purified ~32-kDa protein.


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Fig. 2.   Mass spectrometric identification of p32. The co-purified p32 was excised from the SDS-polyacrylamide gel shown in Fig. 1A, lane 2. The protein band was subjected to trypsin in-gel digestion, and the peptide fragments were analyzed by liquid chromatography MS/MS. The MS/MS data were correlated to the data base of known protein sequences using Sequest. A, peptides of p32 that matched the sequence of cRDH, 11-cis-retinol dehydrogenase (Accession no. A55429), are indicated by lines drawn above the corresponding portion of the sequence. Observed and calculated (in parentheses) mass values for the doubly protonated molecular ion are given above the line. B, high resolution (Zoom) scan used to determine the accurate m/z value and charge state of the ion corresponding to the peptide LLWLPASYLPAR (Mr = 1398.8). C, MS/MS spectrum of the peptide LLWLPASYLPAR that was matched to the protein sequence data base. Members of the B and Y'' fragment ion series (51) are indicated along with their observed m/z values.

Co-purification of Retinol Dehydrogenase Activity-- An assay was performed to verify the presence of enzymatically active cRDH in preparations of RGR. Exogenous 11-cis-retinal was used as the substrate. The reduction of 11-cis-retinal was followed by its disappearance and was dependent on the amount of immunoaffinity-purified RGR added to the reaction (Fig. 3). The activity required the NADH cofactor. The reduction activity of cRDH was found to accompany each step in the purification of RGR and was abolished if RGR was heat-inactivated (results not shown). These observations indicated that cRDH is co-purified with RGR in a detergent solution and remains an active enzyme.


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Fig. 3.   Co-purification of RGR and 11-cis-retinol dehydrogenase activity. cRDH activity in immunoaffinity-purified RGR was shown by enzymatic reduction of exogenous 11-cis-retinal. Various amounts of the eluted RGR (3.8 ng/µl) were incubated with 10 nmol of 11-cis-retinal in the presence of 1 mM NADH for 6 min at 37 °C (100 µl reaction volume). Subsequently, retinal was determined by the thiobarbituric acid method and measured by absorbance at 530 nm (A530). Without NADH, A530 was 0.27 at the 25-µl RGR fraction. In the absence of exogenous 11-cis-retinal, background A530 was 0.01 at the 25-µl RGR fraction.

Stereospecificity and Cofactor Dependence of Co-purified cRDH-- Important features that distinguish cRDH from its counterpart, the tRDH in photoreceptor cells, are substrate stereospecificity and cofactor dependence of the enzyme. These properties of the dehydrogenase were tested in preparations of RGR to further characterize the co-purified cRDH. The cRDH in immunoaffinity-purified RGR reacted with exogenous 11-cis-retinal but not with all-trans-retinal (Fig. 4A). Co-purified cRDH preferred NADH rather than NADPH as the cofactor in the reduction reaction (Fig. 4, A and B).


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Fig. 4.   Substrate specificity and cofactor dependence of 11-cis-retinol dehydrogenase. The reduction activity of co-purified cRDH and RPE microsomal membranes was analyzed in reactions with exogenous retinal isomers. A and B, immunoaffinity-purified RGR (90 ng) or C and D, RPE microsomes (100 µg of protein) were incubated with exogenous all-trans- (×) or 11-cis-retinal (). Each reaction contained 10 nmol of retinal isomer in the presence of 1 mM NADH or NADPH, as shown. The reactions were performed for the indicated length of time at 37 °C (100-µl reaction volume). Retinal was determined by the thiobarbituric acid method and measured by absorbance at 530 nm. A, absorbance at the indicated time point; A0, absorbance at time zero.

For comparison, the activity of cRDH in RPE microsomal membranes was also assayed. As expected, microsomal cRDH was active in the reduction of 11-cis-retinal, and there was only little activity with all-trans-retinal as the substrate (Fig. 4, C and D). The enzyme in the microsomal membranes displayed substrate-specific activity with either cofactor, NADH or NADPH. These observed properties of microsomal cRDH were consistent with the results of previous studies (12-14).

Effect of cRDH on 11-cis-Retinal Bound to Irradiated RGR-- Since cRDH reacts specifically with 11-cis-retinal, the role of co-purified cRDH in RGR function may be to reduce and remove bound 11-cis-retinal from RGR after stereospecific photoisomerization of its all-trans-retinal chromophore. In previous experiments, cRDH reduction activity was not observed because the required cofactor was not added (20).

RGR was kept in the dark or irradiated with blue light. Subsequently, each sample was incubated in the dark in the presence or absence of NADH. The predominant chromophore extracted from nonirradiated RGR was all-trans-retinal, as expected (Fig. 5A), and incubation with NADH had no effect on the all-trans chromophore in the dark (Fig. 5B). The irradiation of RGR and subsequent incubation in the dark without NADH resulted in stereospecific isomerization of ~55% of the bound all-trans-retinal to 11-cis-retinal (Fig. 5C); these results were in agreement with previous studies (20). When RGR was irradiated and then incubated in the dark in the presence of NADH, there was a significant decrease in total retinal. The decrease in total retinal was brought about by the selective loss of 11-cis-retinal with little effect on the amount of the all-trans isomer (Fig. 5D). Changes in the chromophore of RGR were most prominent when NADH was present during irradiation of RGR. Under conditions in which the cofactor is included and cRDH is active, irradiation of RGR resulted in significant loss of both 11-cis- and all-trans retinal (Fig. 6).


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Fig. 5.   Interaction of co-purified 11-cis-retinol dehydrogenase with the chromophore of RGR. Equal aliquots of RGR from the same preparation were irradiated or not for the first 5 min without cofactor. Subsequently, each sample (260 ng of RGR) was incubated in the dark for an additional 5 min in the presence or absence of NADH. Retinal isomers were then extracted by the hydroxylamine derivatization method and analyzed by HPLC. A, incubation in the dark for 10 min without NADH. B, incubation in the dark for 5 min and then incubation in the dark for another 5 min in the presence of 3 mM NADH. C, irradiation for 5 min and then incubation in the dark for 5 min without NADH. D, irradiation for 5 min followed by incubation in the dark for 5 min in the presence of 3 mM NADH. The incubations were performed at 37 °C, and the irradiated samples were exposed to 470-nm monochromatic light. RGR in this and all following experiments was isolated in the presence of 20% glycerol. 11, 11-cis syn-retinaloxime; all, all-trans syn-retinaloxime; 13, 13-cis syn-retinaloxime. In these experiments, the anti isomers of the retinaloximes were below the level of detection.


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Fig. 6.   Irradiation of RGR in the presence of NADH-dependent 11-cis-retinol dehydrogenase activity. Equal aliquots of RGR from the same preparation were irradiated with 470-nm monochromatic light for 5 min at 37 °C in the absence (upper trace) or in the presence (lower trace) of 3 mM NADH (260 ng of RGR/reaction). The retinal isomers were then extracted by the hydroxylamine derivatization method and analyzed by HPLC. 11, 11-cis syn-retinaloxime; all, all-trans syn-retinaloxime; 13, 13-cis syn-retinaloxime. In these experiments, the anti isomers of the retinaloximes were below the level of detection.

Synthesis of 11-cis-Retinol during Irradiation of RGR-- To confirm that 11-cis-retinal was reduced by the co-purified cRDH with retention of the expected isomeric configuration, we demonstrated the production of 11-cis-retinol upon irradiation of RGR in the presence of NADH. The retinoid extracts from irradiated preparations of RGR were analyzed after exposure to light for different lengths of time. Absorbance was measured at 320 nm to optimize the detection of 11-cis-retinol. The irradiation of RGR in the presence of NADH resulted in significant decline of all-trans-retinal and a concomitant time-dependent increase in 11-cis-retinol (Fig. 7). The amount of 11-cis-retinol formed corresponded closely to the amount of all-trans-retinal lost at 5 min; however, the production of 11-cis-retinol lagged the more rapid decline in all-trans-retinal in the first 1-2 min of incubation. Since cRDH uses the 11-cis isomer specifically, we conclude that the increase in 11-cis-retinol is due to the reaction of cRDH on free or bound 11-cis-retinal, the photoisomerization product of irradiated RGR.


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Fig. 7.   Production of 11-cis-retinol by irradiation of RGR in the presence of NADH. Equal aliquots of RGR from the same preparation were irradiated with 470-nm monochromatic light for various lengths of time at 37 °C in the presence of 3 mM NADH (640 ng RGR/reaction). The reactions were stopped, and the retinal isomers were extracted at the indicated times by the hydroxylamine derivatization method. 11-cis-Retinol was co-extracted and analyzed simultaneously by HPLC. Two representative experiments are shown for the decline in all-trans-retinaloxime, corresponding to the all-trans-retinal chromophore of RGR (A), and synthesis of 11-cis-retinol (B). The accumulation of 11-cis-retinol did not occur without NADH. C, HPLC chromatograms of the absorbance (AU) of 11-cis-retinol at 320 nm at various time points of a single experiment. D, representative HPLC chromatograms of the 11-cis-retinol standard (5.5 pmol) and extracted retinoids after 0 and 7-min incubations. The retinoids were detected by absorbance at 320 nm using the Waters HPLC system and were predominantly all-trans syn-retinaloxime and 11-cis-retinol (arrows) after 0 and 7-min incubations, respectively.


    DISCUSSION
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
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DISCUSSION
REFERENCES

The RGR opsin is a major all-trans-retinal-binding protein in the RPE. Physiological evidence that supports the processing of precursor all-trans-retinol and its incorporation into the chromophore of RGR in the dark has been obtained recently (33). Illumination of RGR results in stereospecific conversion of the bound all-trans-retinal to 11-cis-retinal. Since retinoid-binding proteins would be required to transfer the chromophore to or from RGR, the mechanism by which RGR functions may depend on specific protein interactions. In this paper, we demonstrate functional interaction in vitro between the RGR opsin and cRDH. Indeed, the isolation of highly purified RGR from bovine RPE results in consistent co-purification of cRDH in a manner that suggests that cRDH binds to RGR in a protein complex.

In the presence of glycerol, co-purification of cRDH was optimal and Coomassie Blue staining of cRDH was typically as intense and specific as that of RGR in polyacrylamide gels. A general effect of glycerol is to stabilize the protein complex and reduce nonspecific binding to the affinity resin (34). The co-purified enzyme was active and held several properties similar to those of microsomal cRDH (12-14), isolated cRDH (28), or recombinant cRDH (35, 36). It was efficient in the reduction of exogenous 11-cis-retinal and had little reactivity with the all-trans isomer. NADH was preferred over NADPH as a cofactor in the reduction reaction. In contrast, microsomal cRDH activity was observable with both NADH and NADPH (Fig. 4, C and D). Despite the presence in the RPE of cRDH activity that uses NADPH (12, 37), co-purification of the NADPH-dependent enzyme with RGR was not evident.

Most noteworthy was the substrate stereospecificity of cRDH in reactions with the chromophore of RGR. The co-purified enzyme was inert to all-trans-retinal bound to RGR in the dark but active toward endogenous 11-cis-retinal that was generated by irradiation of RGR. The cRDH was remarkably stable during co-purification with RGR over a period of 28 h, and its activity did not require purification of the enzyme in the presence of NADH, as reported previously (28).

The functional significance of RGR and cRDH interaction may lie in the mechanism of chromophore dissociation from RGR. Like squid rhodopsin and other invertebrate visual pigments (38, 39), the chromophore of RGR opsin does not readily dissociate after photoisomerization in vitro, and irradiation does not lead to bleaching of RGR, as determined by difference absorption spectra before and after light exposure (22). If RGR operates in vivo as a stereospecific photoisomerase to directly generate the 11-cis chromophore in the visual cycle, then all-trans-retinal should be photoisomerized and 11-cis-retinal should be released from RGR catalytically. The previous experiments on irradiation of RGR were run without NADH and achieved at most ~50% net conversion of all-trans- to 11-cis-retinal (20). We now can demonstrate more complete photoisomerization of the bound all-trans-retinal to the 11-cis isomer. When RGR is irradiated in the presence of NADH, isomerization of the chromophore is coupled to efficient reduction by cRDH. These results strongly suggest that 11-cis-retinol is generated in a photic visual cycle. Possible steps in further processing of 11-cis-retinol include its conversion to 11-cis-retinyl esters or binding to cellular retinaldehyde-binding protein. The results do not exclude an additional mechanism of dissociation and direct transfer of 11-cis-retinal from RGR to cellular retinaldehyde-binding protein or any effect of cellular retinaldehyde-binding protein on the interaction between RGR and cRDH.

The functional coupling of RGR with cRDH extends the evidence that RGR and rhodopsin have evolved with distinct, yet parallel, features. The chromophores of RGR and rhodopsin are photoisomerized in opposite directions; hence, each is bound to 11-cis- and all-trans-retinal at a specific photochemical state of the opsin. After photoisomerization of the chromophore, 11-cis-retinal from RGR is reduced by cRDH, and all-trans-retinal from rhodopsin is reduced by tRDH to the respective alcohols. The cRDH and tRDH are highly homologous retinol dehydrogenases (6, 35, 36). The reduction of all-trans-retinal participates in the inactivation of rhodopsin and is a rate-limiting step of the visual cycle at high light levels (5). The tRDH is tightly associated with the rod outer segments (40-43); however, its orientation in the rod outer segment membrane and possible binding to rhodopsin are unknown. In comparison, there is evidence that the catalytic domain of cRDH in the RPE is situated toward the lumen of the smooth endoplasmic reticulum (44). This membrane topology of cRDH suggests that 11-cis-retinal from irradiated RGR is reduced by cRDH and presented at the lumenal surface of the smooth endoplasmic reticulum for transport or further processing of the alcohol.

The physiological significance of the 11-cis-retinal reduction reaction involving cRDH and RGR needs to be corroborated by in vivo studies. Another reductase role for cRDH was first proposed by Lion et al. (12). Most models of the visual cycle since then have emphasized the oxidation reaction of cRDH instead, because no experiment to regenerate rhodopsin with 11-cis-retinol in excised mammalian retina has succeeded (45-47). On the other hand, cone pigments in excised retina can be regenerated with 11-cis-retinol (48). In this case, the reduction of 11-cis-retinal from RGR to 11-cis-retinol might be sufficient for chromophore synthesis by the RPE in a cone visual cycle. Alternatively, the 11-cis-retinol from RGR may be converted to the retinyl ester for storage or usage as a requisite intermediate in the synthesis of 11-cis-retinal. Our results provide a new argument for a reductase role of cRDH in addition to its role in oxidation of 11-cis-retinol. The placement of RGR and cRDH in a common pathway may generate additional insights into the molecular defect in cases of fundus albipunctatus that involve mutations in the human cRDH gene (49, 50).

    ACKNOWLEDGEMENT

We thank Wenshan Hao and Daiwei Shen for advice and technical assistance.

    FOOTNOTES

* This work was supported in part by grants from the Hoover Foundation (to H. K. W. F.) and National Institutes of Health Grants EY03040 and EY08364 (to H. K. W. F.) and RR06217 and CA33752 (to T. D. L.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Published, JBC Papers in Press, March 27, 2001, DOI 10.1074/jbc.M010441200

|| To whom correspondence should be addressed: Doheny Eye Institute, 1355 San Pablo St., Los Angeles, CA 90033. Tel.: 323-442-6675; Fax: 323-442-6688; E-mail: hfong@hsc.usc.edu.

    ABBREVIATIONS

The abbreviations used are: RPE, retinal pigment epithelium; RGR, RPE retinal G protein-coupled receptor; cRDH, 11-cis-retinol dehydrogenase; tRDH, all-trans-retinol dehydrogenase; HPLC, high performance liquid chromatography; MS/MS, tandem mass spectrometry.

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
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