Page 37713: Fig. 1,
A and B, was incomplete. The corrected figure is
shown below.
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Fig. 1.
A, schematic structure of BACE-HA
showing signal peptide (SP), propeptide domain
(PD), active site motifs (DTG and
DSG), transmembrane domain (TM), and
hemagglutinin epitope tag (HA). Numbers refer to
amino acid positions; asterisks denote N-linked
glycosylation sites; and the arrow indicates the D93A active
site mutation. The signal peptide and propeptide domain sequence is
shown below in brackets, with the underlined RLPR
representing the furin recognition motif, and the thin arrow
indicating the propeptide domain cleavage site at Glu-46. B,
appropriate propeptide cleavage of the active site mutant D93A BACE-HA.
D93A BACE-HA from transfected 293 cells labeled with
[3H]threonine (left) or
[3H]phenylalanine (right) was radiosequenced. The
figures show radiactivity in counts/min per sequencing cycle. The D93A
BACE-HA radiosequences show a major peak of [3H]threonine in
cycle 2 and a major peak of [3H]phenylalanine in cycle 15, consistent with mature BACE starting at Glu-46. Radiosequencing of
wild-type BACE reveals the same Glu-46 start site (data not shown). The
N-terminal sequence of mature BACE is shown below, with the
cycle 2 threonine and the cycle 15 phenylalanine indicated by
numbers.
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