From the Institut de Génétique et
Microbiologie, CNRS UMR 8621, Université Paris-Sud XI, Centre
Universitaire d'Orsay, Bâtiment 409, F-91405 Orsay Cedex, France
Received for publication, June 30, 2000, and in revised form, November 7, 2000
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ABSTRACT |
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Expression of the structural genes for alcohol
and aldehyde dehydrogenase, alcA and aldA,
respectively, enables the fungus Aspergillus nidulans to
grow on ethanol. The pathway-specific transcriptional activator AlcR
mediates the induction of ethanol catabolism in the presence of a
coinducing compound. Ethanol catabolism is further subject to negative
control mediated by the general carbon catabolite repressor CreA. Here
we show that, in contrast to alcA and alcR, the
aldA gene is not directly subject to CreA repression. A
single cis-acting element mediates AlcR activation of
aldA. Furthermore, we show that the induction of the
alc gene system is linked to in situ aldehyde
dehydrogenase activity. In aldA loss-of-function mutants,
the alc genes are induced under normally noninducing
conditions. This pseudo-constitutive expression correlates with the
nature of the mutations, suggesting that this feature is caused by an
intracellular accumulation of a coinducing compound. Conversely,
constitutive overexpression of aldA results in suppression
of induction in the presence of ethanol. This shows unambiguously that
acetaldehyde is the sole physiological inducer of ethanol catabolism.
We hypothesize that the intracellular acetaldehyde concentration is the
critical factor governing the induction of the alc gene system.
The fungal model organism, Aspergillus nidulans, is
able to grow on ethanol as the sole source of carbon (1, 2). Alcohol is
oxidized via acetaldehyde into acetate, which enters mainstream metabolism in its activated form, acetyl-CoA. The two enzymes responsible for acetate formation, i.e. alcohol
dehydrogenase I (ADHI)1 (EC
1.1.1.1) and aldehyde dehydrogenase (ALDH) (EC 1.2.1.5), are encoded by
the unlinked alcA and aldA genes, respectively. Ethanol catabolism is inducible by a variety of compounds and is driven
by the irreversible conversion of acetaldehyde into acetate.
Induction of ethanol catabolism further requires the action of a
pathway-specific DNA-binding activator protein, AlcR, regulating both
structural genes at the level of transcription (3-7). The encoding
alcR gene, closely linked to alcA, concomitantly
activates its own transcription. This autoactivation enables the
adaptation toward ethanol conversion upon induction. Moreover, the
alcR activation cascade mechanism facilitates a powerful
induction; alcA and aldA are among the most
highly transcribed, inducible fungal genes known to date (1, 8).
Upon addition of more preferable carbon sources, induced ethanol
catabolism ceases. This phenomenon, known as carbon catabolite repression, is likewise mediated at the level of transcription via a
DNA-binding protein, CreA (2, 9). Unlike induction, this negative
control circuit is polytrophic since many catabolic systems are subject
to carbon catabolite repression by means of the CreA repressor. CreA
has been shown to act directly on both the structural alcA
gene and the regulatory alcR gene, ensuring a rapid and
complete shut down of ethanol conversion under repressive conditions
(7, 9, 10). In the presence of both D-glucose and the
gratuitous inducer 2-butanone (ethylmethyl ketone, EMK), the catabolic
repression is so strong that it completely overrules the induction of
all three principal genes for ethanol utilization. However, the two
antagonizing control circuits allow a fine-tuning of the expression of
alcR and alcA within a culture subjected to less
extreme growth conditions (11). A schematic representation of the
regulation of the ethanol catabolic pathway in A. nidulans is shown in Fig. 1.
INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
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Fig. 1.
Schematic representation of the regulation of
the ethanol catabolic pathway in A. nidulans. In
the presence of a coinducer, the AlcR activator is able to activate the
two structural genes alcA and aldA encoding ADHI
and ALDH, respectively (thick arrows). In addition the
alcR gene is subject to positive autoregulation that is
visualized by a curved arrow. The CreA repressor, in the
presence of glucose, directly represses the alcR and
alcA genes (black bars). On the right,
the enzymes involved in the catabolism of ethanol, which is oxidized
via acetaldehyde into acetate, are depicted.
To understand the underlying molecular mechanisms, we have investigated the requirements in cis for AlcR-mediated activation and CreA-mediated repression in the structural alcA gene (12, 13) and the regulatory alcR gene (7, 10). In both genes the in vivo functional cis-acting elements were identified. Multiple AlcR targets in alcA were shown to act synergistically upon transcriptional activation, which could explain the extraordinary strength of the alcA promoter upon induction, whereas alcR only contains a single activation target. Furthermore, repression and induction were shown to be mutually exclusive at the molecular level due to a competition between the two DNA-binding proteins, as their respective cis-acting elements in both the alcA and alcR promoters reside in close proximity. In addition, we have provided evidence for the coexistence of a distinct mode of action by which CreA establishes transcriptional repression of the alcR gene.
In this communication, we analyze the means by which the two regulatory circuits impose their control on the transcriptional induction of the structural aldA gene. aldA has been previously cloned and characterized at the nucleotide level (4, 14, 15). Our current study reveals that at the molecular level, the regulation of aldA transcription differs substantially from that of the two other principal genes of ethanol catabolism.
The control of aldA expression could be important,
considering the second prerequisite for induction, a coinducing
compound. It is generally accepted that ALDH can only catalyze
acetaldehyde oxidation, i.e. it is unable to reduce acetate
(16). This irreversible conversion drives ethanol catabolism (17).
Moreover, acetaldehyde is the first intermediate common to ethanol,
L-threonine, and ethylamine utilization, all processes
provoking induction of the alc gene system (1, 3, 5, 18,
19). The presumed key role of ALDH in multiple catabolic pathways
suggests that the expression of the encoding aldA gene is
likely to be subject to tight control. Both ethanol and acetaldehyde
have been proposed as physiological coinducers of the alc
gene system (3, 20). However, direct evidence of the identity of the
physiological inducer has not been obtained to date. To address the
role of the coinducer in the induction process, we have investigated
how the expression of ALDH affects the induction of the alc
gene system. This approach has led to the clear identification of the
physiological inducer with regard to ethanol catabolism. Furthermore,
we provide evidence here indicating that a subtle control of
aldA and alcA gene expression is important for
the onset and maintenance of an optimal catabolic flow from ethanol.
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EXPERIMENTAL PROCEDURES |
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A. nidulans Strains, Media, Growth Conditions, and Transformation-- A. nidulans strains and transformants used in this study are listed in Table I. Refer to Clutterbuck (21) for gene annotations. Media composition, supplements, and basic growth conditions are as described by Cove (22), unless otherwise stated. Ammonium chloride (10 mM) was normally added as the nitrogen source. Ethanol was use in plates at 1% (v/v), uric acid at 100 mg/liter and L-threonine at 100 mM. Generation of biomass for RNA isolation was by submerged growth in minimal medium, either for 8 h at 37 °C with 0.1% D-fructose or for 24 h at 37 °C with 3% lactose and 5 mM urea as sole carbon and nitrogen sources, respectively. Induction was achieved by adding ethanol or 2-butanone (EMK, ethyl methyl ketone) at 50 mM, and biomass was harvested after a further 2.5 h of incubation (induced conditions). For repressed conditions 1% D-glucose was added simultaneously with the inducer. Noninduced biomass was allowed to grow further on the original growth medium for the aforementioned induction period of 2.5 h. Semicarbazide was administered as a freshly prepared 1 M stock solution of the hydrochloride salt set at pH 7.
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Protoplast generation and transformation of A. nidulans with plasmid DNA were performed as described by Tilburn et al. (23). Aspergillus genomic DNA was isolated according to Specht et al. (24).
Generation of a Strain Constitutively Expressing alcR (TgpdA:alcR)-- BF001 was transformed with bAN8 (13) resulting in the L-arginine prototrophic transformant TgpdA:alcR. Southern analysis indicated that it contained two copies of the chimeric gpdA:alcR gene, integrated at two ectopic loci (not shown). Subsequent Northern analysis showed that this transformant is expressing alcR constitutively at a high level under all applied growth and induction conditions.
Generation of Strains Carrying Mutations in AlcR-binding Sites in
the aldA Promoter (Tma aldA)--
First, two plasmids were constructed
to identify the smallest restriction fragment able to complement the
aldA67 mutation. The first plasmid (pMF369) carries the
2.2-kb NheI (220)/SspI (+1970) fragment from
pAN212 (14) cloned into the XbaI and EcoRV sites
of pBluescript KS+
(Stratagene).2 The second
(pMF371) harbors the larger 2.8-kb SalI
(
768)/SspI (+1970) fragment cloned into the
SalI and EcoRV sites of pBluescript KS+ and
contains the complete upstream region present in pAN212. Both are able
to complement BF111 for growth on ethanol when cotransformed with pFB39
(25) (not shown).
To generate an aldA complementation unit in which the
putative AlcR target has been disrupted, the 1.1-kb SalI
(768)/EcoRV (+349) fragment from pMF371 was subjected to
site-directed mutagenesis according to Kunkel et al. (26).
An oligonucleotide
(5'-GAACAGGTCAGGCGGAGCACATCTTGTTGAG) was used
to introduce four base changes (underlined bases, see also Fig. 3). The
mutations were subsequently verified by nucleotide sequence analysis,
and the NheI (
220)/EcoRV (+349) fragment, carrying the mutations, was re-introduced into pMF371 to yield pMF371m.
Finally, the 2.0-kb ClaI/XhoI fragment of the
A. nidulans urate oxidase (uaZ) gene (27) was
introduced. Both pMF371 and pMF371m were digested with ClaI
(
440) and XhoI (5' of the aldA insert) and
ligated with the uaZ fragment to yield pMM376 (wild type
aldA) and pMM377 (promoter-mutated aldA), respectively.
pMM376 and pMM377 were used to transform strain BF151. The uric acid+ transformants utilized in this study (TaldA and TmaaldA, respectively) contain two copies of the introduced plasmid, integrated at the uaZ locus (results not shown).
Generation of Strains Constitutively Expressing aldA at a High
Level (TgpdA:aldA)--
A plasmid (pCP352) was constructed, which
carries the aldA gene fused to the gpdA promoter
at its initiation codon. The 2.3-kb EcoRI/NcoI
fragment from pAN521, carrying the A. nidulans gpdA promoter (28), was cloned in pLITMUS28 (New England Biolabs). The 5'
region of the coding area of aldA was subsequently amplified with Pfu DNA polymerase (Stratagene), using pAN212 as the
template and two aldA-specific oligonucleotides. One
oligonucleotide (5'-CCCGCTCATCATGACTGATT) was used to introduce a
BspHI site at the initiation codon (
2), whereas the
second, (5'-GCTCTAGAGCAGCGGCCT) created an Xba site 3' of
the intrinsic PstI site (+814). The amplified fragment was digested with BspHI and XbaI and ligated into the
NcoI/XbaI linearized vector containing the
gpdA promoter. The polymerase chain reaction-derived insert
was checked for any polymerase chain reaction-induced mutations by DNA
sequencing. Finally, the 3' region of aldA was introduced by
cloning the 2.6-kb EcoRV (+349)/XbaI fragment
from pAN212 into the gpdA:aldA fusion plasmid, opened at the
intrinsic EcoRV and XbaI sites, 3' of the insert,
yielding pCP352.
pCP352 was introduced in the recipient strain BF111 by cotransformation with pFB39, containing the A. nidulans argB gene. L-Arginine prototrophic transformants were isolated.
Isolation of RNA and Quantitative Analysis-- Total RNA was isolated from A. nidulans as described by Lockington et al. (5) and separated on glyoxal agarose gels according to Sambrook et al. (29).
The 32P-labeled probes used were the entire genes cloned either into the plasmid Bluescript for alcR (19) and alcA (12), or pAN212 for aldA (14), or into pSF5 (30) for actin.
The actin gene is presumed to be constitutively expressed under all conditions used and served as an internal control to normalize the amounts of mRNA present on a single blot. Autoradiographs were exposed for various times to avoid saturation of the film. Densitometric scanning was performed with a Biosoft-Orkis system. The intensities of the signals were also quantified using a PhosphorImager (Molecular Dynamics). Experiments were repeated three times.
Cross-feeding Assay-- aldA mutant strains were inoculated on adequately supplemented minimal medium/ethanol plates. After 2 days of incubation at 37 °C, the alcA mutant BF097 was coinoculated at a distance of 1.5 cm from the aldA15 and aldA67 strains. Cross-feeding could be scored after a further 4 days of incubation at 37 °C.
Characterization of aldA Mutants--
A 2.8-kb DNA fragment,
encompassing the larger aldA complementation unit described
above, was amplified from genomic DNA from mutant strains carrying the
aldA15, aldA57, and aldA67 alleles, respectively, with Pfu DNA polymerase and two
aldA-specific oligonucleotides. One oligonucleotide
(5'-AGTCGTCGACCAAACTGCAAATACAAGTGTACGA) contains the upstream
SalI site (768), and the second
(5'-ATGCTCTAGATAGTGTTCAGCAACCGTGGA) introduces a unique XbaI
site 3' of the downstream SspI site (+1970). Amplified
material was digested with SalI and XbaI and
ligated into pBluescriptKS+. The complete inserts were subjected to
nucleotide sequence analysis. In each case, a single base change was
found with respect to the wild type aldA sequence (see
"Results"). The three mutations were verified upon a second,
independent amplification, in the case of aldA67, utilizing
DNA from a second mutant strain. The nucleotide data from the
wild type gene and the three mutant alleles have been submitted to
GenBankTM under accession numbers AF260123
(aldA), AF260124 (aldA15), AF260125
(aldA57) and AF260126 (aldA67), respectively.
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RESULTS |
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Induction of aldA Transcription Correlates with the
Intracellular Steady State Level of AlcR in the Presence of a
Coinducer--
It has been well established that the aldA
gene has an absolute requirement for a functional alcR gene
and a coinducing compound for induction (3-5, 7). Furthermore,
convincing evidence has been provided that aldA is expressed
constitutively at basal levels independently of AlcR (7, 11). Fig.
2 recapitulates these two distinct
features of aldA expression. A mutant completely lacking
both the alcA and alcR genes (alc500)
is unable to induce aldA transcription regardless of the
presence or absence of a coinducing compound. The deletion strain,
however, exhibits a basal level expression of aldA
comparable with wild type expression under noninduced conditions (Fig.
2A). The converse occurs in a strain in which the
transcription of alcR is driven by the strong, A. nidulans glyceraldehyde-3-phosphate dehydrogenase
(gpdA) promoter (gpdA:alcR), as shown in Fig.
2B. In this strain, aldA is overexpressed due to
the constitutive presence of high steady state levels of the
transactivator protein but only in the presence of a coinducing compound. As expected from our previous work, 2-butanone (EMK) is a
better inducer than ethanol. Induction of aldA thus
correlates with the amount of AlcR present in the cell but always
requires the presence of a coinducer. We confirm also that basal level expression is independent from both AlcR and coinducer. In this respect, aldA expression differs significantly from that of
the other structural gene necessary for ethanol utilization,
alcA.
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Repression of Induced aldA Transcription Is Imposed Indirectly via
CreA-mediated Repression of alcR--
The repression of induction of
aldA is mediated via the transcriptional repressor CreA, as
shown for the first time by Lockington et al. (5). This
could be due solely to CreA-mediated repression of the
transactivator-encoding gene, alcR (9). The AlcR-binding sites in the aldA promoter are shown in Fig.
3. However, results have been described
suggesting that aldA, like alcR and
alcA, is under direct control of CreA (7, 11). To determine
if aldA transcription was also subject to the same control,
transcriptional analysis of aldA expression was performed in
an alcR derepressed genetic context.
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Recently, we generated strains carrying a functional alcR
gene in which cis-acting elements mediating CreA repression
of transcription had been disrupted (10). These derepressed
transformant strains provide the proper means to investigate whether
aldA transcription is subject to direct control by CreA, as
only the regulatory alcR gene is able to escape genuine
repression. Fig. 4 shows that a transformant carrying a disruption of one of the functional CreA targets in alcR (TmC3) induces transcription of the
introduced derepressed alcR gene and the genuine
aldA gene, essentially to the same extent under induced and
repressed conditions, i.e. regardless of the presence or
absence of D-glucose. Essentially identical results were
obtained with derepressed alcR transformants harboring distinct promoter mutations (disrupting either one of the two functional CreA targets in alcR) (results not shown).
Therefore, unlike alcA, repression of aldA
induction occurs solely by CreA-mediated repression of the regulatory
alcR gene.
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AlcR Controls the aldA Promoter via a Unique Palindromic Site-- Considering the relevance of the AlcR transcriptional activator for both the activation and repression of induced aldA expression, we set out to identify the cis-acting element(s) in the aldA promoter. The functional aldA gene resides on a 3.7-kb SalI fragment able to complement the stringent aldA67 mutation for growth on ethanol as the sole carbon source (14).
However, the complementation of the aldA67 mutation also occurs using the smaller 2211-bp NheI/SspI fragment (results not shown). This complementation unit contains 220 bp upstream of the proposed initiation codon of aldA and 383 bp downstream of the proposed termination codon. The 5'-non-coding region from the NheI/SspI fragment, depicted in Fig. 3, harbors two conjugated AlcR sites with the consensus core WGCGG (6, 31), appearing as an inverted repeat separated by two bases. The repeats are present 160 and 171 bp upstream of translation initiation site, respectively. Recent analyses in alcA and alcR have shown that only conjugated alcR consensus sites, organized either as inverted or direct repeats, function as AlcR targets in vivo (10, 12). Previously, a double-stranded oligonucleotide containing the inverted repeat present in the aldA promoter was shown to be bound in vitro by a His-tagged AlcR protein (1:197) (32). We have now tested the in vivo functionality of this sole repeat of the AlcR consensus sites in aldA.
The aldA67 mutation provides an ideal genetic background since, as will be shown later in this paper, the mutant aldA67 gene yields heavily impaired transcript levels. Therefore, we can investigate AlcR-mediated induction of aldA at the level of transcription in this background. The putative AlcR target was modified as follows: three residues within the distal consensus site were changed, whereas the proximal site was mutated at the ambiguous first position (see Fig. 3 and "Experimental Procedures" for details). We know from previous studies (12) that a single AlcR site is not functional in vivo.
Transformants with the aldA mutant promoter
(TmaaldA) were unable to grow on
L-threonine (or ethanol) as shown in Fig. 5A. Note that growth on
L-threonine is under the positive control of the
alcR gene. It requires ADHI as well as ALDH activities (review in Ref. 1). Transcriptional analysis of one of these transformants (Fig. 5B) shows a major loss of
aldA induction.
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The residual aldA induction observed is the consequence of the induction of the host aldA67 allele (which is not expressed under noninduced conditions), in addition to the two extra copies of the mutated aldA promoter gene, which are expressed at a basal level under all conditions. As expected, the alcA and alcR genes remain normally inducible. On the other hand, the control transformant expresses both the introduced aldA gene and the alcA and alcR genes at wild type levels. Hence, the results from growth plates and Northern blots are in agreement; the inverted repeat of sites constitutes the cis-acting element involved in AlcR-mediated induction of aldA and is absolutely necessary for growth on L-threonine or ethanol.
Structural Mutations in aldA Give Rise to Induction of the alc Gene
System Under Normally Noninducing Conditions--
Acetaldehyde is a
catabolic intermediate in ethanol utilization as well as in
L-threonine degradation and in ethylamine deamination (1,
3, 18). It is possible that aldA is subject to multiple pathway-specific activation circuits responding to distinct induction signals. To address the inducing signal in ethanol conversion, we have
investigated the transcriptional behavior of the alc gene system in three existing, allelic mutants in aldA (3, 33). Two of these have been characterized to some extent by Pateman et
al. (3). aldA67 is a stringent mutation that does not
allow growth on ethanol or on L-threonine (Fig.
6D). Conversely, the aldA15 mutation shows a leaky phenotype on ethanol (Fig.
6C). Neither mutant produces detectable ALDH activity. Both
are reported to exhibit some expression of ADH activity under
noninduced, nonrepressed growth conditions. We have also investigated
the aldA57 mutant which shows a stringent phenotype for
ethanol and L-threonine comparable to that of
aldA67 (results not shown).
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We have determined the respective mutations within these three aldA alleles. In all cases, a single point mutation was found within the coding region. In aldA67, a G458A mutation introduces an opal termination codon at the position of Trp-131 (index position number 237).3 The truncated protein is unlikely to harbor enzymatic activity, and aldA67 should be considered as an absolute loss-of-function mutation. In aldA57, G1077A changes Gly-338 (index position 489) into Ser. Gly-338 is one of the few highly conserved residues found among ALDHs (34, 35). The stringent nature of the aldA57 mutation suggests that this Gly residue is indispensable for enzymatic activity. We presume that the mutant produces a full-length but inactive protein. Finally, the leaky phenotype of aldA15 is caused by C922T and A286V (index position 424) in the protein. The sequence similarity directly N-terminal of the catalytic Cys residue (index position 437) is poor even among proteins closely related to A. nidulans ALDH (34). Human liver cytosolic ALDH1 and mitochondrial ALDH2 both carry an Ala at index position 424. However, the deduced amino acid sequences of the ALDHs from Alternaria alternata and Cladosporium herbarum specify a Val (36).
The residual enzymatic activity of the mutant ALDH15 protein is sufficient to allow delayed growth and sporulation on ethanol plates as shown in Fig. 6C. Under such conditions acetaldehyde produced from ethanol is excreted into the medium, giving rise to a cross-feeding phenomenon of structural mutants in alcA when coinoculated on the same plate (33). alc mutants show typical nutrient-starved growth on ethanol plates, sparse mycelial outgrowth and very poor conidiation, similar to that of wild type strains on agar plates without carbon source (37). Cross-feeding is visualized by an increased mycelial density and sporulation of the alcA mutant in the vicinity of the aldA15 mutant, which provides the acetaldehyde supporting this local growth (Fig. 6A). Conversely, aldA67 mutants do not grow at all on ethanol and are unable to cross-feed the alcA mutant (Fig. 6B). Note that mutants in alcR cannot be cross-fed (results not shown), because the aldA gene is not inducible in such strains (cf. "Results").
Fig. 7A shows the
transcriptional behavior of the three principal alc genes in
the structural aldA mutants. Interestingly, lesions in ALDH
lead to induction of the alc gene system under normally
noninducing conditions. The level of this noninduced expression appears
to correlate with the nature of the aldA mutant. The leaky
aldA15 mutant exhibits a modest but clear transcription of
alcA under noninducing conditions. Induction of
aldA and alcR over their respective basal level
expression seen in the wild type strain is likewise apparent.
Conversely, the aldA67 absolute loss-of-function mutant
expresses alcA and alcR to virtually the same
levels in the presence or absence of ethanol. The stringent aldA57 mutant provides an intermediate response. An
intracellular accumulation of a coinducing compound during growth on
the noninducing carbon source lactose could explain the acquired
constitutivity in structural aldA mutants. This typical
phenomenon is termed pseudo-constitutive expression. The inducer
involved has to be an in vivo substrate of ALDH. We presume
that this inducer is acetaldehyde formed for instance by
L-threonine turnover (38) or by constitutive pyruvate
decarboxylase activity (39). Acetaldehyde is the main substrate for
A. nidulans ALDH in vitro (17). A similar
situation occurs in case of the A. nidulans purine
utilization pathway. Loss-of-function mutations in the urate oxidase
(uaZ) gene exhibit a pseudo-constitutive expression of the
structural genes in this pathway due to an accumulation of uric
acid (27, 40).
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The direct correlation between the level of pseudo-constitutive expression and the stringency of aldA mutations suggests that alc gene expression depends on the intracellular concentration of the coinducing compound. This could be confirmed in vivo using the aldehyde scavenger semicarbazide (41). Fig. 7B shows that the level of pseudo-constitutive expression in aldA67 reduces progressively with increasing amounts of semicarbazide in a concentration range that does not affect transcription of the actin gene.
One would expect that the mutated aldA genes are induced concomitantly with the alc genes under both growth conditions in all aldA mutants. However, the nonsense mutation in aldA67 not only results in a truncated protein but also in severely reduced transcript levels (Fig. 7A). This feature enabled us to use aldA67 as a recipient background in the identification of the functional cis-acting element mediating aldA induction, described above. The transcript levels in the aldA67 mutant are comparable with that in the wild type under noninduced conditions but do not represent a basal level in aldA67. The mutant gene is regulated normally by the AlcR transactivator because no aldA transcript can be detected in alcR/aldA67 double mutants (results not shown). It is most likely that the mutant messenger is subject to nonsense-mediated mRNA decay under all tested growth conditions (42, 43).
Constitutive Overexpression of aldA Results in a Suppression of the Induction of the alc Gene System in the Presence of Ethanol-- To address further the apparent relationship between the intracellular coinducer concentration and ALDH activity, we have investigated the effects of a fully constitutive overexpression of the aldA gene on the induction of the alc gene system in the presence of ethanol. For this purpose we constructed a plasmid in which the aldA gene is under the control of the strong constitutive and nonrepressible gpdA promoter (for details, see "Experimental Procedures"). Transformants (TgpdA:aldA) that were able to grow on L-threonine as the sole carbon source, implying that aldA function had been restored, were selected.
Fig. 8 shows a transcriptional analysis
of one of these transformants under two different induced conditions:
ethanol and 2-butanone (EMK), respectively. Interestingly, the
induction on ethanol of the alcR and alcA genes,
found in the wild type, is drastically suppressed in the
gpdA:aldA transformant. Constitutive overexpression of
aldA apparently reduces intracellular accumulation of
acetaldehyde, which normally induces the alc genes, by
converting the acetaldehyde initially formed from ethanol into acetate.
Constitutive overexpression of aldA thus suppresses the
induction of the alc gene system on ethanol. The gratuitous
inducer, EMK, on the other hand, is not a substrate for ALDH and
retains its inducing capacity in the gpdA:aldA transformant.
Hence, for the first time direct evidence is obtained showing that
ethanol is not inducing the alc gene system by itself but
that acetaldehyde formed from ethanol is the physiological inducer of
ethanol catabolism.
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DISCUSSION |
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In this paper we have investigated the requirement of the pathway-specific AlcR transactivator and a coinducing compound for the transcriptional induction of the A. nidulans aldA gene (the structural gene encoding aldehyde dehydrogenase). We have established that all elements required in cis for proper aldA expression reside within 220 bp upstream of the start of translation of the aldA gene. We have identified within that promoter the sole functional target mediating the activation of transcription by AlcR. Like all other AlcR activation targets identified in vivo so far (6, 10, 12), it consists of two adjacent AlcR consensus sites. The target in aldA is organized as an inverted repeat of consensus sites, both with an A at the ambiguous first position (44). Furthermore, by using a transformant strain carrying a derepressed functional alcR gene, we have shown now that aldA itself is not subject to repression by means of the general carbon catabolite repressor CreA. Unlike alcA, the other structural gene required for ethanol utilization, CreA-mediated repression of the regulatory alcR gene completely accounts for aldA transcriptional repression. The sole putative CreA consensus sequence within the aldA promoter, although tentatively eclipsing the single functional AlcR target, is not sufficient to subject aldA directly to carbon catabolite repression. Most physiologically relevant CreA targets characterized in A. nidulans to date consist of two adjacent CreA consensus sites (10, 13, 45, 46).
We have now elucidated the means by which the two antagonizing control circuits, pathway-specific induction and general carbon catabolite repression, impose regulation on the three principal genes of ethanol catabolism (see Fig. 3). In both alcR and aldA, activation is mediated via a single AlcR inverted-repeat target, whereas in alcA three functional targets are present. The targets in alcA have been shown to act in synergy (12), and this could well account for the strength of the alcA promoter upon induction of the alc gene system. However, the aldA promoter should be considered at least as powerful as that of alcA, despite the fact that it contains only one activation target. The direct involvement of the CreA repressor in the expression of alcA and alcR could explain this. Previously, evidence was presented that suggests that the repressor and activator compete for binding on the same promoter region in both these genes under all growth conditions (11). Carbon catabolite repression is a phenomenon that depends on the carbon source catabolic flow. In other words, carbon catabolite repression is never totally absent. As a result, disruption of functional CreA targets in both the alcR and alcA genes does not only lead to a derepression in the presence of D-glucose but also results in overexpression under induced conditions (7, 10, 13). Induction of aldA transcription is regulated in a more straightforward manner since the genuine aldA promoter is only subject to AlcR-mediated activation. The expression of aldA thus reflects the true force of the AlcR activation cascade mechanism mediated via a single cis-acting element.
Additionally, the differences in promoter strength could depend on the composition and the context in which the various functional AlcR targets reside. Previous studies have shown that the core context, spacing between the two half-sites of a target as well as orientation of the half-sites, influence AlcR binding in vitro (32, 44). It has been shown recently that one basic residue (Arg-6) in AlcR, outside the AlcR zinc binuclear cluster, is involved not only in DNA binding but also in transcriptional regulation. The rationale for the reduced transcription of aldA lies in the lack of binding of AlcR to the inverted repeat target when this basic residue is mutated (44). Another possible important consideration is the distance between the activation target and the site where the transcription machinery assembles, whereby the aldA promoter may be more accessible to the general transcription machinery. Indeed, it has been shown recently that nucleosome rearrangement can occur in A. nidulans in response to an induction signal (47). Finally, considering the involvement of ALDH in multiple catabolic pathways, the existence of a second, yet unidentified transactivator for aldA should not be excluded.
The second major finding reported in this paper is that acetaldehyde is the physiological inducer of ethanol utilization in A. nidulans. Our experiments with the aldA mutants indicate that when acetaldehyde (formed from regular cellular metabolism under noninducing growth conditions) is not sufficiently converted into acetate, the alc gene system is induced (cf. Fig. 7A). In the aldA mutants the expression level of alcR and aldA mRNAs in noninduced growth conditions reflects the stringency of the aldA mutation, i.e. the expected accumulation of acetaldehyde. Consistent with this, we recently showed that acetaldehyde can evoke induction when added externally to pregrown cultures.4 Ethanol utilization represents an example of a degradation pathway induced by a catabolic intermediate and not by the growth substrate itself.
Acetaldehyde is considered to be highly toxic (48, 49). Our results pose a challenging new question; how can the cell resolve the inducing and the toxic properties of this intermediate? As noted earlier, aldA67 mutants can still grow on carbon sources like lactose despite the apparent lack of ALDH activity. Therefore, the acetaldehyde accumulated in this mutant might be detoxified by other means. One possibility would be by reduction to ethanol, catalyzed by the alcA-encoded ADHI that is expressed pseudo-constitutively in aldA mutants. Acetaldehyde has been shown to be an excellent substrate for ADHI in vitro; reduction by ADHI is considerably faster than oxidation by ALDH (50). One has to note however that other mechanisms of detoxification may be operational, such as the presence of another ALDH activity that would not be sufficient by itself to support growth on ethanol. In the presence of ethanol as the sole carbon source, limiting amounts of coinducer would trigger the formation of more acetaldehyde since the ADHI reaction equilibrium is fully shifted toward oxidation of ethanol. One of the most prominent features of modest pseudo-constitutive expression in aldA15 is the presence of a considerable alcA transcript level. A preferential induction of alcA in the early stages of the induction process could enable enough accumulation of acetaldehyde to permit an optimal catabolic flow from ethanol. With the increase in intracellular acetaldehyde, the activation system can adapt the expression profile of the responsive genes until a steady state acetaldehyde concentration (below the toxic level) is reached. The presence of three synergistically acting AlcR targets in the alcA promoter together with differences in affinity among the functional AlcR targets in alcA, alcR and aldA could contribute to a preferential alcA induction in response to limiting amounts of acetaldehyde. In support of this, we have previously obtained evidence indicating that the proximal AlcR targets in the alcA promoter are predominant in transcriptional activation (12). Recently, we have shown that mutants, carrying a functional alcR gene but which is not subject to autoactivation (due to the disruption of the AlcR functional target), are perfectly able to grow on ethanol as the sole carbon source (10) even though the induction of the alc-responsive genes is far weaker than that in the wild type. This puts the autoregulation of the transactivator-encoding alcR gene into another perspective. The adaptation in response to increasing coinducer levels apparently does not require induced alcR expression but is facilitated by its endogenous regulation.
Another important question arising from the finding of acetaldehyde as the coinducer concerns the onset of induction. Induction requires acetaldehyde whose production requires ADHI. But expression of ADHI requires induction of alcA by AlcR via acetaldehyde. To enable initial induction of alcA, a constitutive basal level expression of the alcR gene is required, and indeed, alcR basal transcription is substantial and is clearly observed in alcR loss-of-function mutants (7, 10, 11, 31). For the onset of the induction process, the level of acetaldehyde should further increase beyond the conversion capacity of the constitutively present ALDH. Changes in acetaldehyde concentration are important in determining the onset and maintenance of the induction process. Clear evidence is provided by transformants expressing aldA from the strong constitutive gpdA promoter (Fig. 8). Prominent expression of aldA in the early phases of induction, as is the case in such transformants, slows down the build-up of intracellular acetaldehyde. As a consequence, the induction process is suppressed due to a failure to induce alcA sufficiently. This implies that the apparent in situ level of ALDH plays an important role in the transduction of the coinducer signal to the transcriptional activator AlcR.
A subtle control of both aldA and alcA gene
expression, in response to differential intracellular concentrations of
acetaldehyde, could therefore be essential for the onset and
maintenance of an optimal catabolic flow from ethanol.
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ACKNOWLEDGEMENTS |
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We thank Prof. H. N. Arst and Prof. H. M. Sealy-Lewis who kindly provided us with various aldA mutant strains; Dr. C. Velot for helpful comments; and Dr. M. Blight for correcting the English.
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FOOTNOTES |
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* This work was supported in part by CNRS Grant UMR 8621, the Université Paris-Sud XI, and from the European Community Contracts BIO4-CT96-0535 and QLCK3-CT1999-00729.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF260123, AF260124, AF260125, and AF260126.
§ Supported by the Erasmus student exchange program. Present address: Fachbereich Biologie, Philipps-Universität Marburg, Karl-von Frischstrasse, D-35032 Marburg, Germany.
¶ Present address: Laboratoire de Neurogénétique Moléculaire, E. 9913, GENOPOLE, 2 Rue Gaston Crémieux, CP5724, F-91057 Evry Cedex, France.
To whom correspondence should be addressed. Tel.: 33 1 69156328; Fax: 33 1 69157808; E-mail: felenbok@igmors.u-psud.fr.
Published, JBC Papers in Press, December 1, 2000, DOI 10.1074/jbc.M005769200
2 The numbers given in parentheses refer to the position in the aldA sequence (GenBankTM accession number AF260123) with respect to the initiation codon.
3 The index position numbers refer to the ALDH superfamily sequence alignment (34).
4 M. Flipphi, and B. Felenbok, unpublished data.
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ABBREVIATIONS |
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The abbreviations used are: ADHI, alcohol dehydrogenase I; ADH, alcohol dehydrogenase; ALDH, aldehyde dehydrogenase; EMK, ethylmethyl ketone (2-butanone); kb, kilobase pair; bp, base pair.
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