The Internal Repeats in the Na+/Ca2+ Exchanger-related Escherichia coli Protein YrbG Have Opposite Membrane Topologies*

Annika Sääf, Louise Baars, and Gunnar von HeijneDagger

From the Department of Biochemistry and Biophysics, Stockholm University, S-106 91 Stockholm, Sweden

Received for publication, February 23, 2001


    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

We have determined the topology of the Escherichia coli inner membrane protein YrbG, a putative Na+/Ca2+ exchanger with homology to a family of eukaryotic ion exchangers. Our results show that the two homologous halves of YrbG both have five transmembrane segments but opposite membrane orientations. This has implications for our understanding of the function of Na+/Ca2+ exchangers and provides an example of "divergent" evolution of membrane protein topology.


    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

The Escherichia coli YrbG protein belongs to a superfamily of membrane transporters that includes both prokaryotic and eukaryotic Na+/Ca2+ exchangers (1). All proteins in this family have an internal repeat in their membrane domain that presumably has arisen from a primordial gene duplication event. A strongly conserved and functionally important so-called alpha -motif that spans two of the predicted transmembrane helices is present in each copy of the internal repeat (1, 2). Attempts to determine the membrane topology of the mammalian Na+/Ca2+ exchanger have yielded conflicting results (3-5), and it is not clear whether the two conserved alpha -motifs are located on the same or opposite sides of the membrane. Obviously, a resolution of this point will have important functional implications for this class of proteins.

In general, both prediction and experimental determination of membrane protein topology is easier for bacterial than for mammalian proteins, and we have thus undertaken a study of the E. coli family member YrbG. The sequence alignment in Fig. 1A shows clear evidence of the internal repeat, including the two alpha -motifs. Overall, there is ~34% identity between the N- and C-terminal halves of YrbG, and a BLASTP (6) search of the Entrez protein sequence data bank using the N-terminal half (residues 1-155) finds the C-terminal half (residues 172-320) with an E-value of 2 × 10-16 (data not shown).

Interestingly, topology predictions using TOPPRED II (7), TMHMM (8), and HMMTOP (9) suggest a topology with 10 transmembrane segments for YrbG, with the first and second internal repeat having opposite orientations in the inner membrane; see Fig. 1B. This is reminiscent of a recently described case of "divergent" topology evolution where two homologous E. coli inner membrane proteins, YdgQ and ORF193, each with 6 transmembrane segments, were shown to have opposite membrane orientations (10). The predicted topology for YrbG likewise suggests that a primordial protein with 5 transmembrane segments has evolved, after an internal gene duplication, into a protein with 10 transmembrane segments where the first and second halves insert into the membrane with opposite orientations. From this perspective, it is particularly noteworthy that the distribution of positively charged amino acids is different between the two halves of YrbG; in the N-terminal half, Arg and Lys residues are predominantly found in the loops between transmembrane segments 1/2 and 3/4, whereas in the C-terminal half they are more prevalent in the loop preceding the first transmembrane segment and between transmembrane segments 2/3 and 4/5; see Fig. 1B. The YrbG protein may thus be a second example of divergent topology evolution, this time following an internal gene duplication event.

Given the biological importance of the Na+/Ca2+ exchanger family and the topology evolution issue, we decided to experimentally map the topology of YrbG using the PhoA fusion approach (11). Our results provide strong support for the predicted topology with 5+5 transmembrane segments, placing the two alpha -domains on opposite sides of the membrane and suggesting that divergent topology evolution may be more prevalent than hitherto thought.

    MATERIALS AND METHODS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Enzymes and Chemicals-- Unless otherwise stated, all enzymes were from Promega (Madison, WI). T7 DNA polymerase, Taq polymerase, and [35S]Met were from Amersham Pharmacia Biotech. T4 ligase was from Life Technologies, Inc. Oligonucleotides were from CyberGene (Stockholm, Sweden). PhoA antiserum was from 5 Prime right-arrow 3 Prime, Inc. (Boulder, CO). The alkaline phosphatase chromogenic substrate PNPP (Sigma 104 phosphatase substrate) was from Sigma.

Strains and Plasmids-- Experiments were performed in E. coli strains MC1061 (Delta lacX74, araD139, Delta (ara, leu)7697, galU, galK, hsr, hsm, strA) (12) and CC118 (Delta (ara-leu)7697, Delta lacX74, Delta phoA20, galE, galK, thi, rpsE, rpoB, argE(am), recA1) (13). All constructs were expressed from the pING1 plasmid (14) by induction with arabinose.

DNA Techniques-- All plasmid constructs were confirmed by DNA sequencing using T7 DNA polymerase. The yrbG gene was amplified by PCR1 from E. coli JM109 chromosomal DNA using Taq polymerase and the Expand Long Template PCR system (Roche Molecular Biochemicals). By use of appropriate PCR primers, a 5' XhoI and a 3' KpnI site were introduced in the regions flanking the amplified gene. The PCR products were cleaved with XhoI and KpnI and cloned behind the ara promoter in a XhoI-KpnI restricted plasmid derived from pING1 containing a lep gene with a 5' XhoI site just upstream of the initiator ATG and a KpnI site in codon 78. Relevant parts of the yrbG gene were amplified by PCR from the pING1 plasmid with a 5' SalI and a 3' KpnI site encoded in the primers. Finally, the amplified SalI-KpnI fragment carrying the lep upstream region and the relevant yrbG segment was cloned into a previously constructed plasmid (15) carrying a phoA gene lacking the 5' segment coding for the signal sequence and the first 5 residues of the mature protein and immediately preceded by a KpnI site. In all constructs, an 18-amino acid linker (VPDSYTQVASWTEPFPFC) was present between the YrbG part and the PhoA moiety.

Expression of Fusion Proteins-- E. coli strain CC118 transformed with the pING1 vector carrying the relevant construct under control of the arabinose promoter was grown at 37 °C in M9 minimal medium supplemented with 100 µg/ml ampicillin, 0.5% fructose, 100 µg/ml thiamin, and all amino acids (50 µg/ml each) except methionine. An overnight culture was diluted 1:25 in fresh medium, shaken for 3.5 h at 37 °C, induced with arabinose (0.2%) for 5 min, and labeled with [35S]methionine (75 µCi/ml). After 2 min, samples were acid-precipitated with trichloroacetic acid (10% final concentration), resuspended in 10 mM Tris/2% SDS, immunoprecipitated with antisera to PhoA, washed, and analyzed by SDS polyacrylamide gel electrophoresis. Gels were scanned in a FUJIX Bas 1000 phosphorimager and analyzed using the MacBAS software (version 2.31).

PhoA Activity Assay-- Alkaline phosphatase activity was measured by growing strain CC118 transformed with the appropriate pING1-derived plasmid in liquid culture for 2 h in the absence of arabinose and then for 1 h in the presence of 0.2% arabinose (16). Mean activity values were obtained from three independent measurements and were normalized by the rate of synthesis (mean of three experiments) of the fusion protein determined by pulse labeling of arabinose-induced CC118 cells as described above. Normalized activities were calculated as in Equation 1,


<UP>A</UP>=(<UP>A<SUB>0</SUB></UP>×<UP>OD<SUB>600</SUB></UP>×n<SUB><UP>Met</UP></SUB>)<UP>/CPM</UP> (Eq. 1)
where A0 is the measured activity, OD600 is the cell density at the time of pulse labeling, nMet is the number of Met residues in the fusion protein, and CPM is the intensity of the relevant band measured on the phosphorimager.

Topology Prediction and Sequence Alignment-- Topology predictions were done using TOPPRED II (7), TMHMM (8), and HMMTOP (9). Sequence alignments were done using BLASTP and PSI-BLAST (6) at the NCBI website and LFASTA (17) at the Biology Workbench 3.0 website on the Internet. Default parameter settings were used in all cases.

    RESULTS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Determination of the Topology of YrbG by PhoA Fusions-- To determine the membrane topology of YrbG, a series of PhoA fusions were made. As recommended (18), the fusion joints were generally placed near the C-terminal end of the putative periplasmic and cytoplasmic loops; see Fig. 1B. All fusions could be expressed in the phoA- strain CC118 (13), could be immunoprecipitated by a polyclonal PhoA antiserum, and were of the expected sizes; see Fig. 2A. Alkaline phosphatase activities were measured according to Ref. 16 and are shown in Fig. 2B.


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Fig. 1.   A, LFASTA alignment of the N- and C-terminal halves of YrbG. The predicted transmembrane segments are underlined, and the conserved alpha -motifs are boxed. Note that each alpha -motif consists of two parts separated by a short, less well conserved loop. B, proposed topology for YrbG. The two homologous halves are indicated by black and hatched transmembrane segments, respectively, and the two alpha -motifs are shown by thick lines. The number of Lys + Arg residues is indicated in each loop. Each fusion is indicated by its number; the exact position of each fusion joint is given in the legend to Fig. 2.


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Fig. 2.   A, expression of YrbG-PhoA fusion proteins in E. coli CC118 cells. Cells were labeled for 2 min by [35S]Met, and fusion proteins were immunoprecipitated by a PhoA antiserum and analyzed by SDS polyacrylamide gel electrophoresis. Lane 1, YrbG37-PhoA; lane 2, YrbG67-PhoA; lane 3, YrbG104-PhoA; lane 4, YrbG128-PhoA; lane 5, YrbG171-PhoA; lane 6, YrbG209-PhoA; lane 7, YrbG239-PhoA; lane 8, YrbG275-PhoA; lane 9, YrbG300-PhoA; lane 10, YrbG325-PhoA. B, alkaline phosphatase activities for the different YrbG-PhoA fusions. Black bars indicate the measured activities (in Miller units) before correcting for expression levels, and white bars indicate activities after this correction (with the expression level for fusion 1 set to 1; see "Materials and Methods").

The alkaline phosphatase activities are in general in very good agreement with the predicted topology. The only apparent exception is the somewhat high activity of fusion 1 (placed in the loop between transmembrane segments 1 and 2). PhoA fusions directly after the first transmembrane segment in polytopic proteins with an Nout orientation (i.e., with a periplasmic N terminus) often give high activities, and there are now a number of examples where it has been found that more than one transmembrane segment needs to be present to ensure translocation of an N-terminal tail across the membrane (19). Because the hydrophobicity profile is very distinct in this area of the protein, and because all the other fusions are consistent with the predicted overall Nout-Cout topology, it seems likely that the high "uncorrected" activity of the most N-terminal PhoA fusion does not properly reflect the orientation of the first transmembrane segment in the full-length protein.

We conclude that YrbG has a two-domain structure where each domain has five transmembrane segments but where the two domains have opposite orientations in the inner membrane. This also places the two conserved alpha -motifs on opposite sides of the membrane. The 5+5 topology implies that YrbG has undergone a process of "internal" divergent topology evolution after the primordial gene duplication event.

    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

In this study, we have mapped the topology of the E. coli inner membrane protein YrbG, which belongs to a large family of Na+/Ca2+ exchangers that includes both prokaryotic and eukaryotic proteins. Sequence alignment, topology prediction, and experimental topology mapping using PhoA fusions strongly suggest a two-domain structure, each with five transmembrane segments and with opposite orientations in the membrane. As shown in Fig. 1B, this places the two copies of the conserved alpha -motif on opposite sides of the membrane, suggesting that this will also be the case for the eukaryotic family members. Some experimental support for this idea has been obtained previously (5). In terms of three-dimensional structure, this means that proteins in the Na+/Ca2+ exchanger family will have a quasi-symmetric organization relative to the plane of the membrane, somewhat reminiscent of the aquaporins where two copies of the so-called NPA loop, one entering the membrane from the cytoplasmic side and one from the extra-cytoplasmic side, are thought to interact in the middle of the pore (20).

Equally interesting, YrbG provides a clear example of divergent topology evolution where the two homologous halves of the protein have evolved opposite orientations in the membrane. Presumably, the redistribution of positively charged Arg and Lys residues between the different loops in the protein underlies this evolutionary process, as has been suggested earlier for the two homologous E. coli inner membrane proteins YdgQ and ORF193 (10). In fact, the bacterial homologues of YrbG found by a PSI-BLAST search of the Entrez protein sequence data base all have the same predicted Nout-Cout topology with 10 transmembrane segments, except for the Synechocystis homologue (slr0681), which has an extra predicted C-terminal transmembrane segment not present in the other proteins, and all have the same enrichment of positively charged residues in their predicted cytoplasmic loops as seen for YrbG (data not shown).

Whether the postulated primordial YrbG half-protein with five transmembrane segments had a single orientation in the membrane or could insert both ways (Nin-Cout and Nout-Cin) as is the case for, e.g. ductin (21), is unknown. If it had only a single orientation, it is unlikely that a topology with 10 transmembrane segments would be adopted by anything but a very small fraction of the molecules immediately after the gene duplication. Instead, the majority would most likely adopt a so-called "leave one out" topology where one of the hydrophobic segments would not insert across the membrane, and all highly charged loops would remain on the cytoplasmic side (22). A considerable number of mutational events would then be required before most molecules would insert into the membrane with the Nout-Cout topology seen today. In any case, our results underscore the importance of the "positive inside" rule (23, 24) for determining membrane protein topology and suggest that divergent topology evolution or even topology inversion may be a more prevalent phenomenon than hitherto thought.

    ACKNOWLEDGEMENTS

We thank Dr. Kyle Cunningham, Johns Hopkins University, for drawing our attention to the Na+/Ca2+ exchanger family.

    FOOTNOTES

* This work was supported by grants from the Swedish Cancer Foundation and the Swedish Natural and Technical Sciences Research Councils (to G.v.H.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed. Tel.: 46-8-16-25-90; Fax: 46-8-15-36-79; E-mail: gunnar@dbb.su.se.

Published, JBC Papers in Press, March 19, 2001, DOI 10.1074/jbc.M101716200

    ABBREVIATIONS

The abbreviation used is: PCR, polymerase chain reaction.

    REFERENCES
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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
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