Identification of 12 New Yeast Mitochondrial Ribosomal Proteins Including 6 That Have No Prokaryotic Homologues*

Cosmin SaveanuDagger §, Micheline Fromont-RacineDagger , Alexis Harington||, Florence RicardDagger , Abdelkader Namane**, and Alain JacquierDagger DaggerDagger

From the Dagger  Génétique des Interactions Macromoléculaires, CNRS (URA2171),  Génétique Moléculaire des Levures, CNRS (URA2171), and ** Chimie Structurale des Macromolécules, CNRS (URA2185), Institut Pasteur, 25-28 Rue du Dr. Roux, 75724 Paris Cedex 15, France

Received for publication, December 1, 2000, and in revised form, January 18, 2001


    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Mitochondrial ribosomal proteins were studied best in yeast, where the small subunit was shown to contain about 35 proteins. Yet, genetic and biochemical studies identified only 14 proteins, half of which were predictable by sequence homology with prokaryotic ribosomal components of the small subunit. Using a recently described affinity purification technique and tagged versions of yeast Ykl155c and Mrp1, we isolated this mitochondrial ribosomal subunit and identified a total of 20 proteins, of which 12 are new. For a subset of the newly described ribosomal proteins, we showed that they are localized in mitochondria and are required for the respiratory competency of the yeast cells. This brings to 26 the total number of proteins described as components of the mitochondrial small ribosomal subunit. Remarkably, almost half of the previously and newly identified mitochondrial ribosomal components showed no similarity to any known ribosomal protein. Homologues could be found, however, in predicted protein sequences from Schizosaccharomyces pombe. In more distant species, putative homologues were detected for Ykl155c, which shares conserved motifs with uncharacterized proteins of higher eukaryotes including humans. Another newly identified ribosomal protein, Ygl129c, was previously shown to be a member of the DAP-3 family of mitochondrial apoptosis mediators.


    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

In yeast mitochondria, the majority of the characterized ribosomal proteins are essential for protein synthesis (for review see Ref. 1). Homologues of about 18 of the 21 prokaryotic small ribosomal subunit proteins can be identified by similarity searches in the yeast complete genomic sequence. However, the number of ribosomal proteins is larger in the small subunit of yeast mitochondria, estimated to be 33 or 36 (2, 3).

Thus far, yeast mitochondrial ribosomal proteins have been identified either by the study of mutant strains with mitochondrial dysfunction (pet mutants; for review see Ref. 4) or by direct biochemical approaches involving isolation of mitochondria, purification of mitochondrial ribosomes, and protein separation followed by microsequencing (5). Mitochondrial ribosome purification has thus far been technically difficult, because the ribosomal proteins represent only 2-3% of the mitochondrial proteins (2). To date, these approaches have identified only a subset of the total number of mitochondrial ribosomal proteins. In yeast, the eukaryote in which most of the studies were performed, only 14 proteins of the small mitochondrial ribosomal subunit have been characterized experimentally (1, 6).

We were originally interested in the study of the yeast YKL155C gene because it was found in a two-hybrid exhaustive screen to interact with the Prp11 splicing factor (7). To test whether this protein was associated with splicing factors under physiological conditions, we used the recently described tandem affinity purification technique (8) that allows the isolation of protein complexes by two successive affinity purification steps under mild conditions. Instead of containing splicing factors, the purified complex associated with Ykl155c was found to consist of the mitochondrial small ribosomal subunit. We took advantage of this rapid and efficient affinity purification strategy to identify and characterize twelve novel proteins of the small ribosomal mitochondrial subunit.

    EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Plasmids and Strains-- Strains used in this study are listed in Table I. Gene deletions were made by replacing the entire open reading frame by a TRP1 or KanMX6 cassette (9). The strains containing green fluorescent protein (GFP)1 fusion proteins or TAP-tagged fusion proteins were constructed by genomic insertion of the tag together with the TRP1 marker downstream of the affected genes to obtain C-terminally tagged fusion proteins. The Tag/TRP1 markers were generated by polymerase chain reaction either from pFA6a-GFP(S65T)-TRP1 for the GFP fusion (9) or pBS1479 for the TAP-tagged fusion proteins (8) using oligonucleotides designed according to the authors. The YGL129C-containing vector pAH028.1R was constructed by cloning a BamHI/NotI polymerase chain reaction fragment of YGL129C into a pCM190-derived plasmid (10).

                              
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Table I
Yeast strains used in this study

Complex Purification-- Complex purification was done essentially by the method described in detail by Rigaut et al. (8) starting with 2 liters of yeast culture. Polyacrylamide gradient gel electrophoresis was done in the Tris-Tricine system (11). A gradient of 5-20% acrylamide was used in all cases. Protein bands were visualized by Coomassie Blue G-250 staining (12).

Mass Spectrometry-- In-gel digestion of the proteins was performed by the protocol of Shevchenko et al. (13), using bovine trypsin (Roche Molecular Biochemicals). The generated peptides were cleaned on a reversed-phase support using Millipore Zip-Tip C18 or Poros R2 (Perseptive Biosystems). The mixture of peptides was analyzed by matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry on a Voyager DE-STR system (Perseptive Biosystems) using alpha -cyano-4-hydroxycinnamic acid as matrix. The software used for data base searches was a local copy of the MS-Fit 3.2 part of the Protein Prospector package (University of California, San Francisco, Mass Spectrometry Facility). A minimum of four peptides was considered necessary for an accurate protein identification.

Tandem mass spectrometry was performed on a triple quadrupole API 365 mass spectrometer (Sciex) equipped with a nanoelectrospray source (Protana). Protein identification was done using the PeptideScan 3.02 software part of the BioToolBox 2.3 package (PE-Sciex) for Macintosh. Protein data bases were searched for matching entries using the peptide sequence tag methodology (14). Identification of a given protein was considered to be accurate if two or more fragmented peptides gave the same polypeptide match or one fragmented peptide and a peptide mass map identified the same protein.

Fluorescence Microscopy-- Fluorescence microscopy was done using a Leica DMRB fluorescence microscope equipped with a Hamamatsu C4880 cooled digital camera and a 100-watt UV excitation lamp. MitoTracker Red CMXRos from Molecular Probes was used as a specific mitochondrial marker following the manufacturer's instructions.

Informatics-- Image manipulation was done with Photoshop version 5 (Adobe). For sequence homology search, we used the PSI-BLAST 2.1 program (15) from the NCBI web site with an expected E-value of 10 for the first BLAST search. The PSI-BLAST iterations were initiated after manual selection of similar sequences based on the presence of potential sequence motifs. The same homologues were also detected using the precalculated Blastp alignments accessible as the Blink feature of the GenPept entries. A similarity between sequences coming from two different organisms was considered significant if searches starting with either sequence found the other as one of the best matches. The search for yeast homologues of prokaryotic ribosomal proteins was facilitated by the available clusters of orthologous groups (16). Multiple sequence alignments were made using the CLUSTALX program (17) and prepared for publication using GeneDoc version 2.6.

    RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Ykl155c Is Associated with Components of the Small Ribosomal Subunit of Mitochondria-- A yeast strain deleted for YKL155C was viable but unable to grow on glycerol as the sole carbon source (respiration-deficient; data not shown). We constructed the TAP-tagged chromosomal fusion of YKL155C according to Rigaut et al. (8). This construction ensured a natural level of tagged protein expression, and the strain was respiration-competent, indicating that the modified protein was functional. The TAP affinity purification of the Ykl155c-associated complex was performed under standard conditions as described (8).

The proteins that remained associated with the Ykl155c-TAP-tagged protein after the two successive affinity purification steps were separated on a denaturing polyacrylamide gradient gel (see "Experimental Procedures"). After Coomassie Blue staining, about 30 different bands were visible, with various intensities (Fig. 1A, lane 1). This variability of band intensities could result from different factors such as uneven protein recovery after trichloroacetic acid precipitation, dye binding dependent on protein sequence and length, bands that contain more than one protein, components of the complex that are not equimolar, or partial degradation.


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Fig. 1.   Proteins that copurify with Ykl155c are components of the small mitochondrial ribosomal subunit. A, the proteins that were isolated by tandem affinity purification using either a tagged version of Ykl155c (lane 1) or Mrp1 (lane 2) were separated by a Tris-Tricine 5-20% gradient gel electrophoresis. MW, molecular weight marker. Protein band identifications were done by mass spectrometry (see "Experimental Procedures"). B and C, RNA dot-blot analysis of the RNA that copurifies with Ykl155c (lane 1) or Mrp1 (lane 2) using 2 µg of total RNA as a control (T). Hybridization was done using radioactively labeled probes specific for the large subunit 21 S mitochondrial rRNA (B) and the small subunit 15 S mitochondrial rRNA (C).

The bands were excised from the gel, and the proteins were identified by MALDI peptide fingerprinting and nanoelectrospray tandem mass spectrometry (see "Experimental Procedures" and Fig. 2 for an example). A number of the identified proteins were known components of the small subunit of the mitochondrial ribosome, namely Nam9, Mrp4, Mrps28, Mrp2, Var1, Pet123, Mrp51, and Mrp1 (Fig. 1 and Table II). These findings, together with the respiratory phenotype that follows the deletion of the YKL155C gene, strongly suggested that Ykl155c was associated with the small subunit of the mitochondrial ribosome.


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Fig. 2.   Mass spectrometry analysis of the tryptic digest for the band that contained Ygl129c. A, after in-gel digestion the resulting peptides were analyzed by MALDI-TOF mass spectrometry. Marked peaks correspond to tryptic fragments of Ygl129c. The measured masses of the peptides are within 150 ppm of the corresponding predicted peptide masses. B, schematic representation of the peptides covering the Ygl129c. Top, peptides identified by MALDI-TOF. Bottom, peptides unambiguously identified by nanoelectrospray-MS/MS. Center, the thin line represents the protein sequence derived from the coding sequence. The thick sections summarize the sequences covered by peptides identified by MALDI-TOF and nanoelectrospray-MS/MS. No peptide was found between residues 1 and 88; this N-terminal fragment is predicted to undergo proteolytic cleavage (Psort II, Ref. 25). C, part of the MS/MS fragmentation spectrum for the most N-terminal sequenced peptide, which begins at position 89 in the predicted protein sequence. A search using these values as y" in a data base containing all the predicted open reading frame sequences for the yeast genome finds the Ygl129c protein. The matched peptide has the sequence VTPGSLYK. The differences between the masses of the marked peaks correspond to the masses of amino acid residues Gly, Pro, and Thr. D, the fragmentation spectrum for the peptide that is found within residues 400-408 in the predicted sequence of Ygl129c shows a very good signal-to-noise ratio. Fragmentation spectra that were generated for other peptides show a signal-to-noise ratio between the two extremes shown in C and D.

                              
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Table II
Summary of small mitochondrial ribosomal subunit proteins

Ykl155c and Mrp1 Are Associated with the Same Complex-- We wanted to confirm that the complex isolated by affinity purification with the Ykl155c-tagged protein indeed corresponded to the small subunit of the yeast mitochondrial ribosome. Therefore, we examined the protein profile of the affinity-purified complex obtained using the TAP-tagged version of the small subunit mitochondrial ribosomal protein Mrp1 (Mrp1-TAP). When compared, the band patterns of the two purified complexes were identical, with the exception of the tagged proteins, which migrated about 5 kDa higher than their unmodified version as a result of the remaining calmodulin binding domain tag fragment (Fig. 1). The presence of Ykl155c in the complex purified with Mrp1 was confirmed by mass spectrometry. The intensity of the Ykl155c band appears to be significantly lower in the Mrp1-TAP purification. It is possible that part of the tagged form of Ykl155c is not associated with the ribonucleoprotein complex, and therefore additional free protein was purified when using Ykl155c-TAP.

To search for RNA components in the purified complex, the RNAs extracted from the purified fractions were heat-denatured, spotted on a filter, and hybridized with either a 21 S (Fig. 1B) or a 15 S (Fig. 1C) yeast rRNA-specific probe. As expected, the 15 S probe for the small subunit mitochondrial rRNA hybridized specifically to the RNA associated with the Ykl155c and Mrp1 complexes, whereas the 21 S-specific probe did not.

Finally, we analyzed the cosedimentation of Ykl155c with Mrp1 and the 15 S rRNA by ultracentrifugation on a 15-30% sucrose gradient under dissociating conditions (0.5 M salt). Whole yeast soluble crude extracts from the Ykl155c-TAP- and Mrp1-TAP-tagged strains were mixed and loaded on top of the gradient. After ultracentrifugation, the fractions were analyzed for their protein content by denaturing gel electrophoresis and immunoblot specific for the protein A component of the tag. In addition, the RNAs of an aliquot from each fraction were spotted on a filter and probed with the 21 S- or 15 S-specific probe. Cosedimentation of Ykl155c with Mrp1 and the 15 S mitochondrial rRNA is shown in Fig. 3.


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Fig. 3.   Ykl155c is associated with the small subunit of the mitochondrial ribosome. 0.5 ml of a mix of total yeast extracts of strains that contained TAP-tagged versions of Ykl155c and Mrp1 was separated by ultracentrifugation on a 38-ml high salt (50 mM Tris-HCl, pH 7.4, 0.5 M ammonium chloride, 10 mM magnesium acetate, and 1 mM dithiothreitol) 15-30% sucrose gradient in the presence of protease inhibitors (Roche Molecular Biochemicals). The arrow indicates the direction of sedimentation. Western blot analysis (1/5000 dilution of peroxidase-antiperoxidase antibodies from Sigma in phosphate-buffered saline with 0.2% Tween 20 and revealed with an ECL kit from Amersham Pharmacia Biotech) of proteins from fractions 10-29 shows that Ykl155c (A) cosediments with the known small subunit ribosomal protein Mrp1 (B). A substantial amount of both Ykl155c and Mrp1 may be seen in the fractions of the upper part of the gradient and may represent forms of the tagged proteins that are not tightly bound to the ribonucleoprotein particle. C, integrated PhosphorImager signal obtained by dot-blot analysis of the RNAs from fractions 6-29 hybridized to probes specific for the large (black-diamond ) and small (black-square) subunit rRNA. The second peak seen at fraction 25 may represent a small ribosomal subunit depleted in proteins due to the high ionic strength of the ultracentrifugation buffer, as previously reported for bacterial ribosomes (26). The shoulder seen in the large subunit peak most likely results from experimental variations in RNA extraction and sampling.

In addition to the proteins previously described as components of the mitochondrial small ribosomal subunit, we found 12 proteins that had not been previously biochemically characterized. Only 6 of these 12 proteins had clear known ribosomal prokaryotic homologues (Table II).

The nomenclature of the mitochondrial ribosomal proteins is as variable as the methods employed for their characterization, but MRP (for mitochondrial ribosomal protein) is the most frequently used. However, this name does not allow one to discriminate between small and large subunit proteins and thus may be misleading. For example, Mrp2 is not the homologue of the prokaryotic S2 ribosomal proteins but belongs to the S14p family. Accordingly, we preferred to designate the newly identified genes RSMxx genes, where RSM stands for ribosomal small subunit of mitochondria, and xx is the number of the corresponding prokaryotic protein family (see Table II). The genes that encode proteins that are not significantly similar to prokaryotic proteins were named with the same prefix RSM followed by a number that begins with 22, because there are 21 prokaryotic small ribosomal subunit protein families. Thus, the proposed name of the YKL155c gene is RSM22 (Table II).

A large amount of functional data was generated in the course of genetic screenings and functional genomic studies in yeast. Hence, for a number of the identified proteins, some cellular or functional data were already available in public data bases. In addition, for some of the less well characterized proteins, we analyzed the cellular localization of the GFP C-terminally tagged proteins and the respiratory competency of strains disrupted for the corresponding genes. These data are summarized in Table II and described below.

Functional Data on the New Proteins That Belong to Known Ribosomal Protein Families-- Ribosomal protein families S7p and S10p are represented in yeast mitochondria by the corresponding homologues Yjr113c and Ydr041w. Mammalian homologues of S7 (19) and S10 (20) were also recently described as components of the bovine small mitochondrial ribosomal subunit. As expected, the fluorescently labeled yeast proteins localized to mitochondria (Fig. 4).


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Fig. 4.   Mitochondrial localization for novel components of the small ribosomal subunit. GFP(S65T)-tagged genomic versions of the genes were obtained by integrative recombination ("Experimental Procedures"). Each row corresponds to a given strain as indicated on the left. The columns were labeled as follows: Nomarski for differential interference contrast images of cells, Mitotracker for images of a specific mitochondrial marker in living cells, and GFP for the image of the fluorescently tagged proteins in the same cells. The respiratory phenotype of the strains is shown at the right of the figure as an indication of the functionality of the fusion proteins. *, Ygl129c localization was visualized using a plasmid carrying the GFP-tagged version of the gene under a doxycycline-regulated promoter ("Experimental Procedures"). Images were obtained in a diploid strain after 12 h of doxycycline derepression. Dagger , image obtained in a diploid strain obtained by crossing wild-type BMA64 (Matalpha ) and the haploid strain LMA134 (Mata) carrying the chromosomal YNR037C GFP-tagged gene. The LMA134 strain was respiration-deficient and showed no active mitochondria when using the Mitotracker marker. WT, strain not modified, wild type.

The C-terminal quarter of the Yer050c sequence is similar to prokaryotic S18 ribosomal proteins (16). In the case of the haploid strain LMA139 (YER050C-GFP; Table I), no strong mitochondrial signal is visible either in the case of the specific mitochondrial marker or in the case of the GFP (Fig. 4), and the strain is respiration-deficient. Mitochondria are visible in a heterozygous diploid strain (obtained by crossing LMA139 and wild-type BMA64 Matalpha ), which can also grow on glycerol as the sole carbon source, but no GFP signal could be detected (data not shown). Hence, the GFP tag interferes with the function of the protein. Although this precludes the determination of its cellular localization, it shows that the protein is essential for mitochondrial function, because the haploid strain LMA139 is respiration-deficient.

A similar case was observed after the GFP tagging of Ynr037c, a homologue of the S19 prokaryotic ribosomal proteins. However, in a heterozygous diploid strain, we were able to detect a weak GFP signal that matches the mitochondrial marker (Fig. 4, bottom).

Novel Proteins Not Similar to Prokaryotic Ribosomal Components Are Essential for Mitochondrial Function-- Given that six newly identified ribosomal proteins have no obvious similarity with previously known ribosomal proteins, it was important to determine the cellular localization and the involvement of these proteins in mitochondrial function. The result of the cellular localization of the GFP C-terminally tagged proteins is shown in Fig. 4 and summarized in Table II. With the exception of Yjr101w, which was not analyzed, all these proteins were unambiguously localized to the mitochondria. Ykl155c, Ygl129c, Ydr175c, and Yjr101w were previously reported in the literature or public data bases to be essential for mitochondrial function (see Table II for references). We confirmed the direct involvement of Ykl155c in mitochondrial function by showing that the defective growth on glycerol resulting from the deletion of its gene could be complemented by a plasmid expressing a wild-type version of the protein (data not shown). For Yil093c, no previously reported functional data were available. We deleted the gene, which proved to be essential for growth on a medium containing glycerol as the sole carbon source (data not shown). All these data confirmed that the newly identified proteins were localized to mitochondria and/or were essential for mitochondrial function.

    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Affinity Purification of the Ribosomal Subunit Is Complementary to Previously Described Methods-- The number of protein components of the yeast small mitochondrial ribosomal subunit was estimated by mitochondrial purification, preparative ultracentrifugation, and two-dimensional gel electrophoresis to lie between 33 (2) and 36 (3). We used an affinity purification method with tagged versions of yeast Ykl155c and Mrp1 that allowed us to identify, by mass spectrometry, 20 different proteins of this ribosomal particle. Some of the previously described components of the small subunit were not found (Table II). Our affinity-purified complex is unlikely to correspond to a specific particle, distinct in vivo from the bona fide small mitochondrial ribosomal subunit, because of the identical patterns of proteins obtained with either Ykl155c or Mrp1 (a previously described mitochondrial ribosomal protein). Thus, the discrepancies observed between the presented method and previously described techniques might result from the loss of specific proteins during the purification procedure or from our inability to identify some of the components. Conversely, at least Ykl155c, with an apparent molecular mass of 68 kDa, is conspicuously absent from the two-dimensional gels previously published for the mitochondrial small ribosomal subunit in which no proteins with an apparent mass above 60 kDa were revealed. The two approaches may thus be regarded as complementary.

Yeast and Mammalian Mitochondrial Ribosomal Proteins-- An international consortium for the study of mammalian mitochondrial ribosomal proteins has been created recently (Mammalian Mitochondrial Ribosomal Consortium). A number of mammalian homologues of the prokaryotic ribosomal proteins have been identified by using the bovine ribosome as a source for the isolation and purification of the mitochondrial ribosomes (19-22). Mammalian proteins belonging to prokaryotic families S7 (19), S12 (21), and S10 and S15 (20) were described this way. We were able to identify in yeast, along with other mitochondrial homologues of prokaryotic ribosomal proteins, the corresponding proteins for the S7 (Yjr113c/Rsm7) and S10 family (Ydr041w/Rsm10) and also the previously identified S15 homologue, Mrps28 (23).

The analysis of the bovine and yeast mitochondrial ribosome shows that an important number of proteins have no similarity with known ribosomal proteins from prokaryotes. For this group of yeast proteins, significant similarities can be detected with sequences of the relatively distant fungi Schizosaccharomyces pombe. In some cases, similar proteins from other fungi may also be found, and one example is a hypothetical protein from Neurospora crassa (NCBI GenPept data base gi 7899415) that is similar to the yeast Ydr175c/Rsm24 protein. Yjr101w/Rsm26 is similar to the yeast mitochondrial small ribosomal subunit protein Mrp1. Interestingly, both proteins also share similarities with proteins of the superoxide dismutase family.

No prokaryotic homologues of Ykl155c/Rsm22 may be detected, but we found significant similarities of its sequence with hypothetical proteins of yet unknown function in several higher eukaryotes, including humans (Fig. 5). It is tempting to speculate that these proteins are true homologues of the yeast Ykl155c/Rsm22 ribosomal protein and are components of the corresponding mitochondrial ribosomes.


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Fig. 5.   Ykl155c is similar to eukaryotic human, nematode, or fruit fly proteins of unknown function. The presented alignment depicts the conserved motifs of predicted proteins from Saccharomyces cerevisiae Ykl155c (Sc) (gi 6322694), Drosophila melanogaster CG13126 (Dm) (gi 7297582), Homo sapiens (Hs) (gi 10436813), and Caenorhabditis elegans SwissProt P91862 (Ce) (gi 7500302). The sequence identification numbers "gi" are from the NCBI GenPept data base. Similar residues conserved in all sequences are shaded in black, residues that are conserved in three of four sequences are shown in white on gray, and those that are conserved in only two sequences are shaded gray; if large insertions were present, the number of residues is shown between parentheses. The succession of conserved residues of the type CXHX3CXnC is reminiscent of a zinc finger-like structure that may be involved in protein-RNA or protein-protein interactions.

Ygl129c/Rsm23 was found by similarity search to be a member of the DAP-3 family of apoptosis mediator proteins (24). Just like Ygl129c/Rsm23, the mouse mDAP-3 protein is localized into the mitochondrial matrix. Ygl129c/Rsm23 is the yeast sequence most similar to the DAP-3 proteins, but this similarity is weak (17% identity with the mouse mDAP-3). Nevertheless, a BLAST search using the S. pombe Ygl129c homologue sequence (SPBC29A3.15c) also finds the human hDAP-3 sequence. Moreover, mDAP-3 is able to partially complement a mitochondrial DNA loss phenotype observed in yeast strains deleted for YGL129C (24). If Ygl129c/Rsm23 is the true homologue of hDAP-3, these observations would suggest an interesting link between modulation of apoptosis and the mitochondrial protein synthesis machinery.

In conclusion, an efficient affinity purification technique allowed us to characterize novel ribosomal components and to bring to 26 the total number of proteins identified as components of the yeast mitochondrial small ribosomal subunit. These findings and the discovery of the yeast Ykl155c/Rsm22 and Ygl129c/Rsm23 as essential components of the mitochondrial ribosome and members of conserved eukaryotic protein families contribute to extending the classification of mitochondrial ribosomal proteins to three different classes. A first group, which probably exists in every eukaryotic organism, consists of proteins similar to prokaryotic ribosomal components. Another group contain proteins with no prokaryotic homologues but only conserved in related species. Finally, a third group that includes the Ykl155c and Ygl129c protein families comprises mitochondrial ribosomal proteins that are conserved across diverse eukaryotic species.

    ACKNOWLEDGEMENTS

We thank B. Séraphin for providing plasmid pBS1479, V. Galy for help on fluorescence microscopy, L. Decourty for help on strain constructions, P. Legrain and O. Bârzu for critical reading of the manuscript, B. Dujon for discussions, and J.-C. Rousselle for help with mass spectrometry.

    FOOTNOTES

* This work was supported by grants from Groupe d'Interet Publique-Aventis, CNRS, and Ministere de l'Education Nationale (France).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Recipient of financial support from Groupe d'Interet Publique- Aventis.

|| Supported by a grant (EUROFAN2 Bio4-CT97-2294) from the European Economic Community.

Dagger Dagger To whom correspondence should be addressed. Tel.: 33 140 613 205; Fax: 33 145 688 790; E-mail: jacquier@pasteur.fr.

Published, JBC Papers in Press, February 14, 2001, DOI 10.1074/jbc.M010864200

    ABBREVIATIONS

The abbreviations used are: GFP, green fluorescent protein; TAP, tandem affinity purification; Tricine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine; MALDI-TOF, matrix-assisted laser desorption ionization time of flight.

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

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