High glucose inhibits glucose-6-phosphate dehydrogenase via cAMP in aortic endothelial cells.

Zhiquan Zhang, Kira Apse, Jiongdong Pang, and Robert C. Stanton

Page 40046, legend to Fig. 5A: The first sentence should read "the PKA inhibitor H89 causes a stimulation of G6PD" (not "an inhibition of G6PD"). The figure and its correct legend are shown below.



View larger version (16K):
[in this window]
[in a new window]
 
Fig. 5.   A, the PKA inhibitor H89 causes a stimulation of G6PD activity in normal and high glucose. BAEC were treated as in Fig. 1B, incubated with glucose for 24 h, and then exposed to 50 µM H89 for 15 min. G6PD activity was measured. Data were normalized by protein and expressed as means ± S.E. of five separate experiments, each run in triplicate (**, p < 0.005; ***, p < 0.001 compared with control). B, effect of PKA on G6PD activity. BAEC were grown to 80% confluent and then lysed. Lysates were incubated for 30 min at room temperature in the presence of the PKA catalytic subunit in a buffer containing 25 mM Tris/HCl, pH 7.4, 1.3 mM dithiothreitol, and 5 mM Mg-ATP. Units of PKA correspond to picomoles of phosphate/min. Values shown are means ± S.E. of data from three separate experiments (**, p < 0.005; ***, p < 0.001 compared with control). C, high glucose increases phosphorylation of G6PD. BAEC that were 80% confluent were exposed to 32P. Then the glucose concentration was increased. After 3 h cells were harvested and lysed, and G6PD was immunoprecipitated using antibody to G6PD. Following immunoprecipitation, G6PD protein was resolved by SDS-polyacrylamide gel electrophoresis and then exposed to x-ray film. N, normal glucose; H, high glucose. Also the same experiment was done using cells that were transiently transfected with G6PD tagged with the epitope FLAG (data not shown). G6PD was then immunoprecipitated using the antibody directed against the FLAG epitope. The results were identical to those shown above in that high glucose led to phosphorylation of FLAG-G6PD.





This Article
Full Text (PDF)
Purchase Article
View Shopping Cart
Alert me when this article is cited
Alert me if a correction is posted
Services
Email this article to a friend
Similar articles in this journal
Similar articles in PubMed
Alert me to new issues of the journal
Download to citation manager
Copyright Permissions
Google Scholar
Articles by Zhang, Z.
Articles by Stanton, R. C.
Articles citing this Article
PubMed
PubMed Citation
Articles by Zhang, Z.
Articles by Stanton, R. C.


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   Biochemistry and Molecular Biology Education 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.