Helical Stalk Segments S4 and S5 of the Plasma Membrane H+-ATPase from Saccharomyces cerevisiae Are Optimized to Impact Catalytic Site Environment*

Patricia Soteropoulos, Airat Valiakhmetov, Ryota Kashiwazaki, and David S. PerlinDagger

From the Public Health Research Institute, New York, New York 10016

Received for publication, December 11, 2000, and in revised form, January 25, 2001


    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The stalk segments of P-type ion-translocating enzymes are presumed to play important roles in energy coupling. In this work, stalk segments S4 and S5 of the yeast H+-ATPase were examined for helical character, optimal length, and segment orientation by a combination of proline substitution, insertion/deletion mutagenesis, and second-site suppressor analyses. The substitution of various residues for helix-disrupting proline in both S4 (L353P,L353G; A354P; and G371P) and S5 (D676P and I684P) resulted in highly defective or inactive enzymes supporting the importance of helical character and/or the maintenance of essential interactions. The contiguous helical nature of transmembrane segment M5 and stalk element S5 was explored and found to be favorable, although not essential. The deletion or addition of one or more amino acids at positions Ala354 in S4 and Asp676 in S5, which were intended to either rotate helical faces or extend/reduce the length of helical segments, resulted in enzyme destabilization that abolished most enzyme assembly. Second-site suppressor mutations were obtained to primary site mutations G371A (S4) and D676G (S5) and were analyzed with a molecular structure model of the H+-ATPase. Primary site mutations were predicted to alter the site of phosphorylation either directly or indirectly. The suppressor mutations either directly changed packing around the primary site or altered the environment of the site of phosphorylation. Overall, these data support the view that stalk segments S4 and S5 of the H+-ATPase are helical elements that are optimized for length and interactions with other stalk elements and can influence the phosphorylation domain.


    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The plasma membrane H+-ATPase of yeast is an essential enzyme that actively pumps protons across the cellular membrane in order to maintain intracellular pH and the electrochemical proton gradient necessary for growth and development. It belongs to the superfamily of P-type ion-translocating ATPases for which there are more than 150 members known (1). The fungal H+-ATPase is a class II, non-heavy metal-transporting enzyme that includes the plant H+-ATPase and the animal Na+,K+-ATPase, Ca2+-ATPase, and H+,K+-ATPase (2). It couples ATP hydrolysis in the cytoplasmic domain to ion transport in the membrane-embedded domain forming an acyl-phosphate intermediate during catalysis. Recently, the molecular structure of the Ca2+-ATPase of skeletal muscle sarcoplasmic reticulum (SERCA1a)1 was solved at 2.6-Å resolution (3). As expected, it confirmed a membrane transport domain with 10 transmembrane segments, a large cytoplasmic ATP hydrolysis domain, and a narrow stalk domain that links the cytoplasmic and membrane domains. The cytoplasmic region consists of three well separated domains, with the phosphorylation site in the central catalytic domain assuming a fold similar to haloacid dehalogenase and the adenosine-binding site formed 25 Å away on another domain (3). These essential features are conserved among P-type enzymes despite wide ranges in amino acid conservation between family members. A detailed comparison of 8 Å structures (4) of the H+-ATPase from Neurospora (5) and the Ca2+-ATPase from sarcoplasmic reticulum (6) confirmed that important structural properties are highly conserved.

There is extensive evidence that dynamic changes in protein structure occur during catalysis (7-9). However, it is not clear how conformational changes in the catalytic region are transmitted to the transmembrane domain. It has been suggested that positional changes in the stalk segments could play a role in mediating the coupling process (10, 11). In the Ca2+-ATPase, the stalk region is ~24 Å long and is divided into four largely helical, rod-like structures (3, 6, 12). The stalk densities are not as apparent in the 8-Å Neurospora H+-ATPase structure, which likely reflects an open conformation being used to derive structural information (5). Nonetheless, the helical stalk region is expected to provide a direct physical linkage between functional domains involved in ATP hydrolysis and ion transport.

The H+-ATPase stalk segments, designated S2 (Gln164-Val189), S3 (Gly270-Asn291), S4 (Val348-Val372), and S5 (Gly670-Tyr689), are the cytoplasmic extensions from transmembrane segments M2, M3, M4 and M5, respectively. Segments M4-6 have been implicated in ion binding/release (13). S4 and S5 are presumed to play a pivotal role in coupling because they are contiguous with these transmembrane segments and are directly linked to the nucleotide binding-phosphorylation domain (2, 11). S4 is within 10 residues or 2.5 helix turns from the site of phosphorylation, Asp378. A mutation in the Ca2+-ATPase, Y763G, near the membrane interface of S5 uncouples ion transport from ATP hydrolysis (14). In the Ca2+-ATPase structure, M5-S5 is a long, centrally oriented, contiguous helical segment that forms a "mast-like" element extending from the outer membrane surface to the center of the cytosolic domain (3).

We previously demonstrated that the helical properties of S2 and S3 are important to enzyme function (15). In this report, we have extended that analysis to examine the importance of helical character in S4 and S5, which are more directly linked to coupling of ion transport. In addition, we have examined spatial interactions of S4 and S5 by identifying second site suppressor mutations that reverse the defective phenotypes observed from point mutations within the stalk segments. Our results strongly support the view that the helical properties of stalk segments S4 and S5 are important for H+-ATPase function, and the length of these segments as well as the juxtaposition of helical faces are optimized to interact with the phosphorylation domain.

    EXPERIMENTAL PROCEDURES
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Yeast Strains-- Primary site mutations generated in vitro were transformed into yeast strain SH122 (HO ade6-1 trp5-1 leu2-1 lys1-1 ura3-1 pma1Delta ::LEU2/PMA1) (16) or SY4 (MATa ura3-52 leu2-3 112 his4-619 GAL1::PMA1::URA3 s6-4ts GAL2) (17). Some suppressor mutations were isolated in the strain YAK2 (MATalpha ade2-101 leu2Delta 1 his3-Delta 200, ura3-52 trp1Delta 63 lys2-801 pma1Delta ::HIS3 pma2-Delta ::TRP1) (18), as indicated.

Site-directed Mutagenesis-- Site-directed mutants were created in plasmids pGW201 (19), pRS201Delta URA3, and Ycp2HSE-PMA1 (17) using the QuickChange Site-directed Mutagenesis Kit (Stratagene). For chromosomal integration, a 6.1-kilobase pair HindIII fragment containing a desired pma1 mutation linked to URA3 was transplaced into yeast strain SH122. Isogenic pma1 mutants were isolated as described by Harris et al. (16). All transformations were performed using the Alkali-Cation Yeast Transformation Kit (Bio 101).

pma1 mutants were grown in a 3-ml YPD (1% yeast extract, 2% peptone, 2% dextrose, pH 5.7) culture for 18-20 h, and chromosomal DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega). The PMA1 structural gene was amplified as a 3.5-kilobase pair fragment by the polymerase chain reaction using flanking primers (5'-GCTCCCCTCCATTAGTTTCG and 5'-GCGTGTTGTGAATTGTGC). PCR products were purified by the Wizard PCR Preps Purification System (Promega). All mutations were verified by DNA sequence analysis of PCR-amplified products.

Generation of Suppressor Mutations-- Suppressor mutations were generated by treating vector pRS201Delta URA3 containing the primary site mutation with 1 M hydroxylamine HCl, 50 mM sodium pyrophosphate, pH 7.0, 100 mM sodium chloride, 2 mM EDTA for 1-2 h at 75 °C. Hydroxylamine was removed from the plasmid using the Wizard PCR Preps Purification System (Promega). Additional suppressor mutations were generated by passing the plasmid through an XL1-Red mutator Escherichia coli strain (Stratagene). The mutagenized plasmids were used to transform the strain YAK2 (18), as described above. The transformed YAK2 were cured of their primary plasmid by plating on media containing 0.1% 5-fluoroorotic acid, 0.67% (w/v) yeast nitrogen base without amino acids, 0.2% (w/v) CSM-URA, 2% (w/v) dextrose, 50 µg/ml uracil, 0.1% 5-fluoroorotic acid, 2% agar). After 5 days of growth, colonies were isolated and suppressors selected on the basis of full or partial reversion to wild type growth. Plasmid DNA was isolated using the Wizard Plasmid Purification Kit (Promega), and suppressor mutations were located by DNA sequence analysis of the entire gene.

Growth Phenotypes of pma1 Mutants-- Resistance to hygromycin B was determined on YPD agar plates containing 200 µg/ml hygromycin B (20). pH-dependent growth measurements were made on YPD agar at pH 2.5 with 20 mM acetate added or in liquid YPD with 20 mM acetate and the pH ranging from 2.5 to 8.0, as described previously (21). Growth rates were evaluated by inoculating a 50-ml culture of YPD (to A590 ~0.2) with stationary phase cultures of pma1 mutants and growing cells with shaking at 30 or 37 °C. Aliquots (0.3 ml) were removed every hour, and the optical density at 590 nm was determined.

Kinetic Properties of the pma1 Mutants-- Plasma membranes were isolated from cells grown to mid-log phase at 30 °C, as described previously (22). Secretory vesicles were isolated as described Nakamoto et al. (17). ATP hydrolysis assays were performed in 96-well microplates, as described in Wang et al. (19). The pH dependence of ATP hydrolysis was determined in a standard reaction medium with the pH adjusted to 4.5-8.5. Vanadate sensitivity was measured in a standard reaction containing 0-100 µM sodium vanadate. Kinetic parameters Km and Vmax were determined by measuring ATP hydrolysis with equimolar (1:1) complexes of ATP and MgSO4 from 0 to 10 mM. Glucose-dependent medium acidification by carbon-starved cells was examined, as described previously (23).

Other Procedures-- All mutations were identified by DNA sequence analysis at the New York University Medical Center Sequencing Facility. Protein concentrations were determined using the Coomassie Plus Protein Assay Reagent (Pierce). SDS-gel electrophoresis was performed using pre-cast 10% minigels (NOVEX). Relative protein abundance measurements were made by gel electrophoresis and Western blot analysis, as described previously (21).

    RESULTS
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Targeted Proline Mutagenesis of S4-- Scanning proline mutagenesis has been used to probe the helical properties of stalk segments S2 and S3 (15). This approach was extended to assess the importance of helical backbone structure in S4 and S5 by making strategic substitutions in largely non-conserved residues near the ends of the two stalk segments. Table I shows the positions of these mutations and their affects on cell viability, H+-ATPase-dependent growth phenotypes, hygromycin B resistance, low pH sensitivity (20, 23), enzyme kinetics, and sensitivity to orthovanadate. The substitution of proline in S4 for residues Leu353 and Ala354 near the membrane interface of M4 and at Gly371 resulted in defective enzymes incapable of supporting growth. A L353G mutation was also highly disruptive. In each case, the introduction of other amino acid substitutions produced active enzymes, which demonstrated that the disruptive propensity of proline, and in some cases glycine, was most likely due to its affect on backbone structure and not on amino side group character. An S368P substitution was viable, although the cells grew slowly and the enzyme displayed less than 50% of normal catalytic activity (Table I). Several mutations (e.g. S368F) at this position are known to induce >500-fold decreases in vanadate sensitivity (16), although the viable S368P mutant was fully vanadate-sensitive (Table I). However, a G371A mutation did show ~10-fold less vanadate sensitivity consistent with a recent study of this region (24). The viable mutant enzymes, L353I, A354G, G371A, and S386P, showed normal Km values and sensitivity to vanadate and, other than S368P, showed Vmax levels that were >= 78% of wild type enzyme.

                              
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Table I
Growth phenotypes and kinetic properties of glycine/proline pma1 mutants

The prominent growth defects induced by the S368P mutation were most pronounced when assessing the proton transport properties of this mutant. Fig. 1 shows a detailed profile for pH-dependent growth in which the S368P mutant was most sensitive to acidic medium conditions. This enzyme appeared less competent in regulating intracellular pH via the H+-ATPase. The kinetic defect in this enzyme was apparent when examining whole-cell proton efflux mediated by the H+-ATPase from carbon-starved cells (Fig. 2). In this case, S368P pumped protons routinely at a rate 55-65% (n = 3) that of wild type enzyme or other mutants, which is consistent with its reduced Vmax in vitro. Mutant enzymes A354G and G371A were somewhat less efficient (15-20%) than wild type (Fig. 2). Interestingly, both of these enzymes showed rates of ATP hydrolysis that were >92% of wild type (Table I), suggesting that they may be partially uncoupled. Most of the other mutants showed wild type-like rates of H+-ATPase-mediated proton transport.


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Fig. 1.   pH-dependent growth of pma1 mutants. Mutant cells (103) were inoculated into rich YPD medium containing 20 mM potassium acetate and were grown for 24 h at 30 °C. Each point represents the average of cells grown in triplicate.


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Fig. 2.   H+-ATPase-mediated medium acidification. Carbon-starved cells were incubated in weakly buffered medium, and H+-ATPase-mediated proton efflux was assessed by following medium acidification with the pH indicator dye bromphenol blue. Changes in the initial linear rate of acid efflux relative to wild type (GW201) were taken as differences in the rate of H+-ATPase-mediated proton efflux. Each point is the average of three measurements.

Targeted Proline Mutagenesis of S5-- In the Ca2+-ATPase, M5 and S5 form a contiguous, long central helical element (3) that has been proposed to play a critical role in the coupling reaction (25). In contrast to the Ca2+-ATPase and related higher eukaryotic enzymes, the fungal H+-ATPase has a naturally occurring proline, Pro669, as well as a Gly670, near the M5 interface, which should disrupt helical continuity in this region and create hinge-like flexibility. The conversion of Pro669 to glycine and alanine had modest effects on enzyme function (Table I), suggesting that backbone flexibility is not important at this position.

In contrast, the introduction of proline at both Asp676 and Ile684 at opposite ends of S5 resulted in highly defective enzymes incapable of supporting growth. As observed with S4 mutations, the introduction of either glycine or alanine at these positions was well tolerated by the enzyme (Table I). These results suggest that helical character of S5 is important for function. Mutations D676R and D676G, but not D676A, produced enzymes with low rates of ATP hydrolysis, hygromycin B resistance, and low pH sensitivity indicative of a highly defective enzyme (Table I; Fig. 1). Whole-cell proton transport assays also confirmed that these mutant enzymes were less efficient in H+-ATPase-directed proton transport (Fig. 2). It is likely that side group mass at this position is more important than charge.

Suppressor Mutations of G371A and D676G-- The prominent growth characteristics of mutants G371A in S4 and D676G in S5 permitted the isolation of second-site suppressor mutations to evaluate short and long range protein-protein interactions. Spontaneous suppressor mutants were readily isolated but generally resulted in gene replacement events that restored the wild type PMA1 gene. To promote the generation of second site suppressor mutations, plasmid pRS201Delta URA3 containing a primary site mutation was either chemically mutagenized by treatment with hydroxylamine or passaged through a bacterial mutator strain (see "Experimental Procedures"). Suppressors to G371A were found at M405I and P535L, both located within the catalytic domain, and T608I, located in stalk segment S5. One suppressor mutation of D676G resulted from two second site mutations at E288K and V562I, which are located in Stalk segment S3 and the catalytic domain, respectively. The two other D676G suppressors were found at V748I in the cytoplasmic turn between membrane segments M6 and M7 and G888S in transmembrane segment M9. All suppressor mutations restored phenotypic wild type growth and yielded enzymes that had rates of ATP hydrolysis that were 100% of wild type for G371A and 81% for D676G. Whole-cell proton transport assays also indicated that the suppressor mutations largely restored wild type proton transport function (not shown).

Characterization of Deletion and Alanine Insertion Mutants-- The helical character of the stalk elements was further probed by single residue deletion and sequential four amino acid insertions (i + 1 ... i + 4) around Asp354 in S4 and Asp676 in S5. The deletion and alanine insertion mutants were expressed in a secretory vesicle system (17) to better determine the biochemical properties of the mutant H+-ATPase enzymes. Fig. 3 shows the level of assembled enzyme and rates of ATP hydrolysis for the mutant enzymes. The data indicate that both S4 and S5 are highly sensitive to changes in the helical axis. In all cases, except for a single alanine insertion in S4, the deletion and addition mutations were highly disruptive resulting in an unstable enzyme that assembled poorly, even in the Sec vesicles. These mutants were highly reminiscent of assembly defects encountered with mutations of the phosphorylation site, Asp378 close to S4 (26). A single alanine addition was tolerated resulting in a largely assembled enzyme (65% of wild type) with only 35% of wild type enzyme activity.


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Fig. 3.   Effect of addition/deletion mutations on enzyme assembly and activity. Addition and deletion mutants were evaluated in enzymes expressed in secretory vesicles. The membrane vesicles were purified, the proteins separated by SDS gel electrophoresis, and the level of H+-ATPase evaluated with a whole anti-H+-ATPase antibody. Densitometric scans were used to visualize the bands (A). ATP hydrolysis was evaluated in quadruplicate in purified vesicles (B).


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The P-type ATPase stalk region is widely viewed as a mediator of energy coupling between the catalytic ATP binding/hydrolysis and ion translocation domain. Genetic evidence from several systems support this view by demonstrating that mutations in several stalk elements either partially or fully uncouple ion transport from ATP hydrolysis. These mutations include I183A in S2 (19), G158D near the S2/M2 interface (27), H285Q in S3 (28) of the yeast H+-ATPase, and Y763G in the S5 (14) of the Ca2+-ATPase. The Y763G mutation in S5 has been proposed to directly modulate coupling via an affect on M5/M6, which has been implicated in ion binding (11, 29). Furthermore, a K758I mutation in the Ca2+-ATPase leads to changes in the rates of dephosphorylation and Ca2+ binding (30), and perturbations in S3 produced by either mutation or the binding of the inhibitor thapsigargin interfere with energy coupling (31).

In this work, the helical character and length of stalk segments S4 and S5 of the yeast H+-ATPase were shown to be important for enzyme function and stability. The substitution of various residues for helix-disrupting proline (or in some cases glycine) in both S4 (L353P,L353G; A354P; and G371P) and S5 (D676P and I684P) resulted in highly defective or inactive enzymes (Table I). In each case, substitutions with residues other than proline restored normal or near-normal enzymatic function (Table I). Deletion or addition of one or more amino acids at positions Ala354 in S4 and Asp676 in S5, intended to either rotate helical faces or extend/reduce the length of helical segments, were in most cases deleterious resulting in enzyme destabilization that significantly decreased enzyme assembly (Fig. 3). The assembly defects observed were comparable to those observed by mutation of Asp378, the site of phosphorylation, and neighboring residues (26, 32). These results indicate that S4 and S5 play more than a simple structural role bridging the catalytic phosphorylation and nucleotide binding domains to the membrane-embedded ion transport domain. Rather, the helical elements are juxtaposed or packed in an optimal manner that is not highly flexible.

Scanning alanine mutagenesis of S4 by Ambesi et al. (24) supports the notion that side group interactions are essential in this segment. A sided preference was observed for inactivating mutations from Lys362 to Leu375 which may define potential interaction sites. These authors further demonstrated that various mutations in consecutive residues Ile359 through Gly371 produced enzymes with significantly reduced sensitivity to orthovanadate. Similarly, G371A in this study showed a 10-fold reduced vanadate sensitivity. This behavior is linked to a shift in E1-E2 equilibrium toward the vanadate-insensitive E1 conformation. It is supported by previous studies on vanadate insensitivity (16, 33), by related studies on yeast H+-ATPase M4 (34) and S4 (24), as well as S4 in the Ca2+-ATPase. In the latter study, mutations in the S4 region altered E1-E2 and/or Ca2+·E1P right-arrow Ca2+·E2-P transitions suggesting that this segment links phosphorylation and Ca2+ binding (35). Collectively, these results suggest that perturbations in S4 are linked directly to changes in the disposition of the site of phosphorylation Asp378.

In the 2.6-Å resolution structure of the Ca2+-ATPase, S5 and M5 form a long continuous alpha -helical structure of ~60 Å that spans the membrane domain and a significant portion of the cytoplasmic domain. It is worth noting that a naturally occurring proline, Pro669, occurs at the interface of S5 and M5 in the yeast H+-ATPase. This proline could disrupt the continuity of the extended M5-S5 helical segment observed in the Ca2+-ATPase. However, it is likely that the distortion imposed by the naturally occurring proline is minimal since Pro669 can be converted to alanine thereby restoring helical integrity with little affect on enzyme behavior (Table I). This behavior may reflect the fact that Pro669 lies at the interface of helical elements constrained by different dielectric environments and/or through close interactions with other stalk and membrane elements.

The directed proline mutagenesis, helix lengthening, and shortening and twisting of stalk segment S5 in this work supports the importance of an optimized helical element. This likely results from the location of specific residues on one or more faces of the helix to form critical interactions. In fact, saturation mutagenesis of S52 revealed two faces of the helix that tolerate mutation poorly and may be potential regions of interaction. Assorted S5 mutations in the Ca2+-ATPase alter the rate of the reaction sequence HnE2 right-arrow Ca2E1 associated with Ca2+ binding on sites at the cytoplasmic face sites and on the rate of the dephosphorylation of the ADP-insensitive phosphoenzyme, HnE2P right-arrow HnE2 (25). S5 is likely to play a pivotal role in mediating communication between the Ca2+-binding pocket and the catalytic domain. In addition, a critically conserved residue, Arg751, appears important for both structural and functional integrity of the enzyme (25). S5 is linked directly to the transmembrane ion binding domain composed of M4-M6, and M8, through its connection with M5.

The intramolecular rearrangement of stalk segments during catalysis remains obscure. The analysis of second site suppressor mutations (SSSM) enables short and long range interactions within a protein to be explored. In the current context, the presumptive behavior of primary and secondary site mutations were analyzed by superposition of the yeast H+-ATPase primary sequence on conserved portions of the molecular coordinates of the SERCA Ca2+-ATPase (Fig. 4). Primary site mutation G371A lies on the edge of stalk 4, seven residues upstream of the site of phosphorylation Asp378 (Fig. 4). This mutation introduces a slightly bulkier side group at this position suggesting some steric crowding. Given its proximity to Asp378 and the finding that mutations in this region influence the phosphorylation state of the enzyme (32), it is likely that the G371A mutation alters the environment around Asp378 via main chain interactions involving twisting or other distortions. The introduction of proline at this position was highly destabilizing resulting in a non-viable enzyme (Table I). Second site mutations appear to have both short and long range effects that in each case stabilize the main chain containing Asp378. SSSM T680I lies on an adjacent descending chain and shows close contact with the primary site mutation G371A. The side group change may relieve some steric crowding introduced by the primary site mutation. SSSM P535L lies within the highly conserved sequence D534PPR, which has been linked to coordination of Mg2+ ions (36). This side group and backbone change may compensate for the primary site effect by directly impacting the environment around Asp378, perhaps by making it more favorable for phosphate transfer through a decreased polarity. SSSM M405I lies 27 residues downstream of the phosphorylation site. In the Ca2+-ATPase structure, this residue lies at the end of an elongated chain that turns abruptly back toward the membrane. It may exert its effects via main chain twisting, but there is no indication that the chain is sufficiently structured to facilitate such a molecular distortion. Rather, in the H+-ATPase, it appears more likely that M405I actually lies closer to Asp378 because the next 62 of 82 residues, including the immediately following 34 residues of the SERCA pump, are absent in the yeast enzyme (Fig. 4). This gap is predicted to bring M405I much closer to Asp378 in which the isoleucine side group can more directly impact the environment of the site of phosphorylation. Overall, it is suggested the primary impact of the G371A mutation is to alter the environment around Asp378, and the role of the second site suppressor mutations is to restore a more normal environment either by changing the immediate environment or altering the main chain distortion.


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Fig. 4.   Putative locations for primary and secondary site mutations. The primary amino acid sequence of yeast PMA1p was overlaid on the molecular structure coordinates for the SERCA Ca2+-ATPase (A) by an iterative single residue matching process and visualized with the InsightII (Molecular Simulations, Inc.) structure modeling package. The backbone trace of the SERCA pump is shown with conserved PMA1 residues (26.4% identity; 37.3% similarity) shown in green (identical) and orange (similar), non-conserved residues shown in black, and residues absent in the PMA1p enzyme shown in dark gray. B and C represent a topside view of the cytoplasmic portion of the enzyme without the membrane portion displayed and show the backbone position of second site suppressor mutations (green dots) obtained for primary site mutations G371A and D676G (red dots), respectively. The highly conserved 11-amino acid stretch (Ile374-Thr384) main chain, containing the site of phosphorylation, Asp378 (blue dot), is designated by the solid arrow (blue-gray). The structure was displayed using Swiss-PDBViewer version 3.61 (Glaxo Wellcome).

Primary site mutation D676G on stalk 5 is complemented by SSSM V748I, G888S, and E288K,V562I. Asp676 is upstream of the highly conserved Asp634 and Asp638, which are presumed to help coordinate nucleotide-bound divalent cation (37, 38). One likely effect is that the D676G mutation alters this coordination. SSSM E288K maps to stalk segment S3 that lies in the same horizontal plane as D676G, suggesting a close interaction during catalysis. SSSM V748I maps to the cytoplasmic region between M6 and M7 further suggesting another potential interaction with S5. Finally, V562I lies just outside the conserved motif LTGD560. In the haloacid dehalogenase family, the highly conserved residue, Ser118, which is equivalent to PMA1 Thr558, is believed to form a hydrogen bond with substrate carboxylate (37, 38). The V562I mutation may help increase this interaction, which was diminished by the primary site mutation D676G. In this scenario, changes in S5 are strongly linked to changes in nucleotide binding at the catalytic site.

The wide distribution of second site suppressor mutations clustering on protein structure elements that directly or indirectly impinge upon the catalytic Asp378 suggests that the conformational dynamics of the enzyme are highly dependent on the phosphorylation state of this residue, as expected. More generally, this work suggests that second site suppressor studies, when coupled with a working molecular structure model, can yield valuable information about local protein structure interactions and dynamics. Such studies can be especially useful when evaluating interactions occurring as a result of a primary site mutation that alters a short lived conformational state.

Overall, the results in this study provide evidence that stalk segments S4 and S5 of the yeast H+-ATPase are optimized helical elements that are closely linked to phosphorylation events occurring within the catalytic site.

    FOOTNOTES

* This work was supported by National Institutes of Health Grant GM 38225 (to D. S. P.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Public Health Research Institute, 455 First Ave., New York, NY 10016. Tel.: 212-578-0820; Fax: 212-578-0804; E-mail: perlin@phri.nyu.edu.

Published, JBC Papers in Press, February 5, 2001, DOI 10.1074/jbc.M011115200

2 M. Miranda and C. W. Slayman, unpublished observations.

    ABBREVIATIONS

The abbreviations used are: SERCA, skeletal muscle sarcoplasmic reticulum; PCR, polymerase chain reaction; SSSM, second site suppressor mutations.

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

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