From the Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724
Received for publication, October 11, 2000
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ABSTRACT |
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The nucleation of RNA polymerases I-III
transcription complexes is usually directed by distinct multisubunit
factors. In the case of the human RNA polymerase II and III small
nuclear RNA (snRNA) genes, whose core promoters consist of a proximal
sequence element (PSE) and a PSE combined with a TATA box,
respectively, the same multisubunit complex is involved in the
establishment of RNA polymerase II and III initiation complexes. This
factor, the snRNA-activating protein complex or
SNAPc, binds to the PSE of both types of promoters
and contains five types of subunits, SNAP190, SNAP50, SNAP45, SNAP43,
and SNAP19. SNAPc binds cooperatively with both Oct-1, an
activator of snRNA promoters, and in the RNA polymerase III snRNA
promoters, with TATA-binding protein, which binds to the TATA box
located downstream of the PSE. Here we have defined subunit domains
required for SNAPc subunit-subunit association, and we show
that complexes containing little more than the domains mapped here as
required for subunit-subunit contacts bind specifically to the PSE.
These data provide a detailed map of the subunit-subunit interactions
within a multifunctional basal transcription complex.
The basal transcription machineries that recruit RNA polymerases
I-III to promoters are all composed of large multisubunit complexes.
Such complexes are well suited to combinatorial mechanisms of
transcription regulation because they provide for a large amount of
flexibility by having many protein surfaces accessible for interactions
with transcription activators and repressors. Thus, the same
multisubunit complexes are used at many different promoters, but in
each case different subsets of protein surfaces are functional. The
small nuclear RNA
(snRNA)1-activating protein
complex SNAPc (1, 2), also called PTF (3, 4), is an
especially interesting example because SNAPc is involved in
the nucleation of both RNA polymerase II and RNA polymerase III
transcription initiation complexes.
The human RNA polymerase II and III promoters of small nuclear RNA
genes are quite similar in structure, and they recruit many of the same
transcription factors (see Ref. 5 and references therein). The enhancer
region of these genes contains several binding sites, one of which is
always an octamer sequence. The octamer is bound by the POU domain
transcription activator Oct-1. The core RNA polymerase II snRNA
promoters consist of a single essential element, the proximal sequence
element (PSE), whereas the core RNA polymerase III snRNA promoters
consist of both a PSE and a TATA box. In both types of promoters the
PSE recruits the multisubunit complex SNAPc, and in the RNA
polymerase III snRNA promoters, the TATA box recruits the TATA-binding
protein (TBP) (2, 6). TBP is also required for RNA polymerase II transcription from snRNA promoters, but in that case, it is not clear
how TBP is recruited to the promoter (7, 8).
SNAPc, which contains five types of subunits, SNAP190,
SNAP50, SNAP43, SNAP45, and SNAP19 (5), is known to be involved in a
number of interactions. The complex binds to the PSE, and this requires
a Myb domain present in SNAP190, as well as the SNAP43 and SNAP50
subunits (9, 10). On probes containing both an octamer motif and a PSE,
binding to DNA is cooperative with Oct-1. This cooperative binding
contributes to transcription activation and depends on a direct
protein-protein contact involving a small region surrounding lysine 900 in the SNAP190 subunit of SNAPc and glutamic acid 7 in the
Oct-1 POU domain (11-13). In the RNA polymerase III U6 snRNA promoter,
SNAPc also binds cooperatively with TBP (14) and is likely
to contact directly the transcription factors hB" and hBRFU, which are
both required for U6 transcription (15).
Because much is known about SNAPc, and because functional
SNAPc can be reconstituted from recombinant subunits, it
offers a unique system to understand the functions of the various
surfaces of a basal transcription multisubunit complex. It also offers a unique opportunity to understand how such a complex is assembled. Toward these goals, we have mapped subunit-subunit contacts within SNAPc. We show that we can assemble a complex missing
SNAP19, SNAP45, and large segments of SNAP190 and SNAP43 that is still capable of binding to DNA. Together, these results reveal the detailed
architecture of a DNA-binding complex capable of nucleating both RNA
polymerase II and III transcription initiation complexes.
Translations in Vitro--
The various truncated open reading
frames were subcloned into derivatives of the expression vector pCite
(Novagen). All SNAP190 truncations were fused to an HA tag at the N
terminus of the protein, whereas the C-terminal SNAP43 truncations were
fused at their C terminus to the last 14 aa of SNAP43, which are
recognized by the anti-SNAP43 antibody anti-CSH375 (1). The pCite
constructs were used as templates for in vitro transcription
and translation with the TNT T7 coupled reticulocyte lysate system
(Promega L4610). Two µg of DNA template were mixed with 25 µl of
TNT rabbit reticulocyte lysate and (i) 2 µl of reaction buffer, (ii)
1 µl of 1 mM amino acids minus methionine mix, and (iii)
1 µl of 40 units/µl RNasin ribonuclease inhibitor, all supplied by
the manufacturer, and 2 µl of [35S]methionine at 10 mCi/ml in a total volume of 50 µl, and incubated for 30 min at
30 °C. For proteins that contained few or no methionines, the 1 µl
of 1 mM amino acids minus methionine mix was replaced by 1 µl of 1 mM amino acids minus cysteine mix, and the 2 µl
of [35S]methionine at 10 mCi/ml were replaced by 2 µl
of [35S]cysteine at 10 mCi/ml.
Immunoprecipitations--
Five µl of the relevant in
vitro translated proteins were mixed with 10 µl of protein
G-agarose beads (packed bed volume) covalently coupled to the 12CA5
monoclonal antibody, which recognizes the HA tag (16), or 10 µl of
protein A-agarose beads (packed bed volume) covalently coupled to the
anti-SNAP43 anti-CSH375 antibody (CS48), which recognizes the last 14 aa of SNAP43 (1), or 10 µl of protein A-agarose beads coupled to the
anti-SNAP50 antibody (anti-CSH482, CS303 (17)), in 500 µl of
HEMGT-150 (25 mM HEPES (pH 7.9), 0.2 mM EDTA,
12.5 mM MgCl2, 10% glycerol, 0.1% Tween 20, 150 mM KCl, 1 mM dithiothreitol, 0.5 mM phenylmethylsulfonyl fluoride, 1 µM
pepstatin A, 0.5 mM bisulfite, and 0.5 mM
benzamidine), and the mixture was rotated for 1 h at room
temperature. The beads were then washed four times with HEMGT-150,
pelleted, and resuspended in 25 µl of 2× Laemmli buffer (125 mM Tris (pH 6.8), 20% glycerol, 4% SDS, 2%
2-mercaptoethanol, 0.01% bromphenol blue). The beads were boiled for 3 min and pelleted, and the supernatant was loaded onto a 15%
SDS-polyacrylamide protein gel. The
[35S]methionine-labeled proteins were then detected by autoradiography.
Assembly of SNAPc Subcomplexes--
Open reading
frames encoding various SNAPc subunits or truncated
subunits were subcloned into derivatives of the bacterial expression
vector pSBet (18) and expressed in BL21 bacterial cells according to
Studier et al. (19). The SNAP190 derivatives contained six
histidines fused to the C terminus. The subcomplexes were formed by
mixing the relevant soluble protein lysates for 2 h at 4 °C.
Each complex was purified by a 20-45% ammonium sulfate precipitation
followed by chromatography on Ni2+-nitrilotriacetic
acid-agarose beads (Qiagen). The various subcomplexes were analyzed for
DNA binding in an electromobility shift assay with a probe containing a
high affinity mouse U6 PSE or a mutated PSE as described by Mittal and
Hernandez (14).
By determining the abilities of various SNAPc subunits
translated in vitro to coimmunoprecipitate (2, 5, 9, 17), and by assembling SNAPc subcomplexes in insect cells
infected with recombinant baculoviruses overexpressing different
SNAPc subunits (10), we have established a crude map of
protein-protein contacts within SNAPc, which is shown in
Fig. 1. From such experiments we know
that SNAP19 and SNAP45 can each associate with SNAP190 and that SNAP43
can associate with SNAP50. SNAP43 can also associate with SNAP190, but
in coimmunoprecipitations of in vitro translated proteins,
this association is not detectable unless SNAP19 is present. This
suggests that SNAP43 has weak contacts with both SNAP190 and SNAP19,
only the sum of which is measurable by the stringent
coimmunoprecipitation assay. To map more precisely the regions of
protein-protein contacts within SNAPc, we expressed truncated or mutated versions of various SNAPc subunits by
translation in vitro, and we determined their ability to
coimmunoprecipitate with other SNAPc subunits. We first
concentrated on the contacts between SNAP190, SNAP19, and SNAP43.
INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
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Fig. 1.
Architecture of SNAPc. The N
and C termini of SNAP190 are indicated. See text for a description of
the known subunit-subunit interactions in SNAPc. Note that
the stoichiometry of the various subunits is not known.
A Small SNAP190 Region Extending from aa 84 to 133 Is Sufficient
for Association with Both SNAP19 and SNAP43 Together with
SNAP19--
To define the SNAP190 region required for association with
SNAP19 alone or SNAP43 together with SNAP19, we constructed a series of
SNAP190 truncations, all of which carried the HA epitope fused to the N
terminus of the protein. These HA-SNAP190 truncations were either
cotranslated or mixed after translation with SNAP19 or SNAP19 and
SNAP43. The mixture was then used for immunoprecipitations with either
the monoclonal anti-HA antibody 12CA5 (16) or an antibody directed
against SNAP43 (1). We then checked the immunoprecipitates for the
presence of SNAP19, or SNAP19 and SNAP43. Fig.
2 shows the SNAP190 truncations that were
tested in these coimmunoprecipitation assays, with the results
summarized to the right of the figure. Fig.
3 shows a selection of some of the
results obtained.
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We first tested the ability of SNAP19 to coimmunoprecipitate with HA-tagged SNAP190 truncations lacking increasing amounts of C-terminal sequences. The results are shown in Fig. 3A. The HA-SNAP190 truncations as well as SNAP19 could be expressed by in vitro translation (lanes 1-7). SNAP19 was coimmunoprecipitated with HA-SNAP190 truncations lacking C-terminal sequences up to aa 133 but not up to aa 92 (compare lane 13 to lanes 8-12). The anti-HA antibody did not immunoprecipitate SNAP19 in the absence of HA-SNAP190 (lane 14). Fig. 3B shows coimmunoprecipitations of the same HA-SNAP190 C-terminal truncations, SNAP19 and SNAP43. In vitro translated SNAP43 (lane 8) was mixed with cotranslated HA-SNAP190 truncations and SNAP19 (lanes 1-7), and the resulting mixtures of proteins were used for immunoprecipitations with the anti-SNAP43 antibody. HA-SNAP190 truncations lacking C-terminal sequences up to aa 133 but not up to aa 92 were coimmunoprecipitated with SNAP19 and SNAP43 (compare lane 14 to lanes 9-13). In no case did we see coimmunoprecipitation of SNAP43 with either SNAP190 or SNAP19 alone, consistent with our previous observation that in this assay SNAP43 associates efficiently with SNAP190 only in the presence of SNAP19 (2). As expected, the anti-SNAP43 antibody did not immunoprecipitate SNAP19 or HA-SNAP190 in the absence of SNAP43 (lane 15). An HA-SNAP190 truncation extending from aa 63 to 121 (SNAP190-(63-121)) could also associate with SNAP19 and SNAP43 together with SNAP19 (data not shown but see Fig. 2). Together, these results indicate that SNAP190 sequences C-terminal of aa 121 are dispensable for association with both SNAP19 and SNAP43 together with SNAP19.
We then deleted increasing amounts of N-terminal sequences from the HA-SNAP190-(1-133) truncation and, in the case of the largest N-terminal deletion, removed the first 92 aa from the HA-SNAP190-(1-261) truncation. We tested the resulting proteins for coimmunoprecipitation with SNAP19 or SNAP43 and SNAP19. As shown in Fig. 3C, all HA-SNAP190 truncations as well as SNAP19 and SNAP43 were expressed by in vitro translation (lanes 1-5 and 11-15). SNAP19 was coimmunoprecipitated with HA-SNAP190 N-terminal truncations missing up to the first 83 aa, but not the first 92 aa (compare lane 9 to lanes 6-8). Similarly, SNAP19 together with HA-SNAP190 truncations missing up to the first 83 aa, but not the first 92 aa, could be coimmunoprecipitated with SNAP43 (compare lane 19 with lanes 16-18). Interestingly, although both the HA-SNAP190-(84-133) and the HA-SNAP190-(63-121) truncations could coimmunoprecipitate SNAP19 and SNAP43 together with SNAP19, an HA-SNAP190-(84-121) truncation could coimmunoprecipitate SNAP43 together with SNAP19 but not SNAP19 alone (not shown but see Fig. 2). Thus, the smallest SNAP190 truncation capable of association with both SNAP19 alone and SNAP43 together with SNAP19 is the SNAP190-(84-133) truncation.
Amino Acid Changes on the Same Face of a Putative -Helix in
SNAP190-(84-133) Disrupt Association with SNAP19 and SNAP43 Together
with SNAP19--
When the SNAP190 protein sequence is analyzed by
programs that predict
-helical regions likely to form coiled coils,
such as COILS (20), the residues from aa 97 to 123 receive high scores (default parameters, 0.726-0.831 with a window of 14 and 0.995 with a
window of 28). Overlapping this region are leucines and glutamines
(circled in Fig.
4A) separated by 6 aa and thus
predicted to reside on the same surface of an
-helix. To determine
whether these residues contribute to association with SNAP19 alone or SNAP43 and SNAP19, we changed them in pairs to alanines. The location of these double aa changes (L87A/Q94A, L101A/L108A, Q115A/L122A) and other mutations described below, as well as a summary of their effect on HA-SNAP190 association with SNAP19 alone or both SNAP43 and
SNAP19, are shown in Fig. 4A, and the results are shown in Fig. 4B. In the context of the SNAP190-(84-133) truncation,
all three double amino acid changes reduced association with SNAP19 to
undetectable levels (Fig. 4B, compare lanes 8-10
with lane 7). When the HA-SNAP190 mutants were mixed with
SNAP43 and SNAP19, very low levels of the HA-SNAP190 mutants and SNAP19
were recovered in an anti-SNAP43 immunoprecipitation (compare
lanes 13-15 with lane 12), suggesting that the
HA-SNAP190 mutants were still able to associate with SNAP19 together
with SNAP43 but only very weakly.
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We then introduced four more mutations. The double mutation
L89A/V96A changes two residues that are out of register with the leucines and glutamines mutated above and are, therefore, predicted to
reside on a different face of the -helix. The single aa changes Q94A, Q94L, and Q115L change the glutamines on the face of the helix
analyzed in Fig. 4B to either leucines or, in one case, alanine. As shown in Fig. 4C, the three single aa changes
reduced HA-SNAP190-(84-133) association with SNAP19 below detection
level (compare lanes 10-12 with lane 8). In
sharp contrast, the out of register mutation L89A/V96A had only
a small effect on association with SNAP19 (lane 9). The
L89A/V96A mutation had also a minimal effect on association with both
SNAP43 and SNAP19, as did the single amino acid changes Q94A and Q115L
(lanes 15, 16 and 18). The Q94L mutation had the
most severe effect on association with SNAP43 together with SNAP19, but
association was still clearly detectable (lane 17).
Together, these data indicate that in general association with SNAP19
alone is more sensitive to mutations than association with both SNAP43
and SNAP19. This is not unexpected and is consistent with the idea that
SNAP43 contacts weakly both SNAP19 and SNAP190 and thus stabilizes the
SNAP190-SNAP19 interaction. The results also support the idea that
SNAP190 adopts an
-helical structure within at least part of the
region analyzed, one face of which is involved in protein-protein
contacts with SNAP19 and SNAP43. The region is not a typical leucine
zipper, however, as replacement of the two glutamines with leucines is deleterious.
Mutations in the N-terminal Half of SNAP19 Disrupt Association with
SNAP190 and SNAP190 Together with SNAP43--
We then turned our
attention to SNAP19. The N-terminal half of SNAP19 contains five
leucines separated by 6 aa that could potentially form a leucine zipper
(2), and indeed, this region receives very high scores when analyzed
with the COILS program (default parameters, 0.907-0.961 between aa 4 and 20 with a window of 14; 0.906 to 0.999 between aa 1 and 36 with a
window of 28) (20). We suspected, therefore, that this region may
interact with the putative -helix in SNAP190. To test this
possibility, we introduced a number of single and double amino acid
changes, whose locations and whose effects on association with SNAP190 or SNAP43 together with SNAP190 are summarized in Fig.
5A. The results are shown in
Fig. 4, B and C. The mutations L8A, L15A/L22A, and L29A/L36A all modify leucines that are part of the putative leucine
zipper. In contrast, L18A modifies a leucine residue that is out of
register with the leucine zipper, and thus on another face of the
putative
-helix. All mutated versions of SNAP19 were efficiently
expressed by in vitro translation, but some of the mutations
significantly retarded the migration of the protein in an
SDS-polyacrylamide gel (Fig. 5B, lanes 1-6). When tested for association with HA-SNAP190-(84-133) alone, all mutations had a
dramatic negative effect except for the L18A mutation, which is out of
register with the leucine zipper and which had little or no effect
(Fig. 5B, lanes 7-12). When tested for association with
SNAP43 together with HA-SNAP190-(84-133), both double mutations had a
strong negative effect, whereas the L8A and L18A mutations still
retained some activity (Fig. 5C, lanes 7-12). Together, these results suggest that the N-terminal region of SNAP19 assumes an
-helical structure with one face, encompassing Leu-8 to at least
Leu-29 and perhaps Leu-36, important for contacts with SNAP190, and the
region from Leu-15 or perhaps Leu-22 to Leu-29 or Leu-36, important for
contacts with SNAP43 together with SNAP190.
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A SNAP43 Region Extending from aa 164 to 268 Is Sufficient for
Interaction with SNAP19 and SNAP190--
To determine which region of
SNAP43 is required for interaction with SNAP19 and SNAP190, we
constructed a number of SNAP43 truncations. Fig.
6A shows the location of these
truncations and a summary of their ability to interact with SNAP19 and
SNAP190 (1st column). Some of the results for association
with SNAP19 and SNAP190 are shown in Fig. 6B. The SNAP43
deletions were in general difficult to translate in vitro,
and several constructs gave rise to a number of bands (see for example
in Fig. 6B, lanes 2, 3, 5, and 6). We mixed the
various SNAP43 deletions with SNAP190-(1-261) and SNAP19, and we used
an antibody directed against the C terminus of SNAP43 for
immunoprecipitation. SNAP190-(1-261) migrated well above the SNAP43
bands and was therefore easily visible on the gels, but SNAP19 (marked
with white arrowheads in Fig. 6B) migrated close
to some of the SNAP43 bands and was therefore more difficult to see
(see Fig. 6B). Nevertheless, by comparing the
immunoprecipitated bands (lanes 8-14) with the bands
present in the starting materials (lanes 1-7), it was
possible to obtain unambiguous results. Full-length SNAP43
coimmunoprecipitated SNAP19 and SNAP190-(1-261) (see Fig. 3B,
lane 10), but neither the N-terminal (SNAP43-(1-185)) nor the
C-terminal (SNAP43-(187-368)) half of SNAP43 did (Fig. 6B, lanes
10 and 11). We therefore truncated increasing amounts
of N-terminal sequences from full-length SNAP43, and we found that a
truncation missing the first 163 aa (SNAP43-(164-368)) was still active (not shown, see Fig. 6A). Thus, the N-terminal border
of the region required for association with SNAP19 and SNAP190-(1-261) is located between aa 164 and 187. C-terminal truncations showed that a
SNAP43 protein ending at position 268 coimmunoprecipitated SNAP19 and
SNAP190-(1-261) (Fig. 6B, lanes 9), but one ending at
position 227 did not (Fig. 6B, lane 13, and data not shown; see Fig. 6A). We then constructed a SNAP43 truncation
extending from 164 to 268 and, as shown in lane 12 of Fig.
6B, this truncation efficiently coimmunoprecipitated
SNAP190-(1-261) and SNAP19. Thus, this SNAP43 region is sufficient for
association with SNAP190-(1-261) and SNAP19.
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The First 168 aa of SNAP43 Are Sufficient for Association with SNAP50-- SNAP43 associates directly with SNAP50 (17), and we therefore checked the abilities of the various SNAP43 truncations to associate with SNAP50. The results are summarized in Fig. 6A (2nd column), and some of the results are shown in Fig. 6C. When full-length SNAP50 was mixed with full-length SNAP43 and immunoprecipitated with an anti-SNAP50 antibody, SNAP43 was coimmunoprecipitated (Fig. 6C, lane 10). Similarly, the N-terminal half of SNAP43 extending from aa 1 to 185, but not the C-terminal half extending from aa 187 to 368, was coimmunoprecipitated with SNAP50 (lanes 11 and 12). A further truncation containing aa 1-163 could also associate with SNAP50 (not shown). Truncations removing aa from the N terminus showed that deletions of the first 32 aa of SNAP43 did not prevent association with SNAP50 (lanes 14 and 15), but deletion of the first 57 aa did (not shown, see Fig. 6A). Thus, the N-terminal border of the SNAP43 region required for association with SNAP50 lies between aa 33 and 58. These data also show that SNAP43 sequences between aa 33 and 163 are required for association with SNAP50 although, because we did not test whether a SNAP43 truncation extending from aa 33 to 163 can still associate with SNAP50, we do not know that these sequences are sufficient. Nevertheless, these results delimit sequences required for association with SNAP50 and show that they are completely separate from the sequences required for association with SNAP190 and SNAP19.
A Small SNAP190 Region Extending from aa 1281 to 1393 Is Sufficient
for Efficient Association with SNAP45--
We have shown before that
SNAP45 associates directly with the C-terminal half of SNAP190 (10). To
map more precisely the SNAP190 region required for this association, we
generated the HA-tagged SNAP190 truncations shown in Fig.
7A, and we tested them for
association with SNAP45 in immunoprecipitations with the anti-HA 12CA5
monoclonal antibody. The results are summarized in Fig. 7A
and some of them are shown in Fig. 7B.
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As we observed previously (10), a SNAP190 truncation encompassing aa 800 to 1469 could associate with SNAP45 (not shown, Fig. 7A). We then tested separately the N-terminal half and C-terminal half of this SNAP190 truncation; the N-terminal half extending from aa 800 to 1176 did not coimmunoprecipitate SNAP45, whereas the C-terminal half extending from aa 1177 to 1469 did (not shown, Fig. 7A). When this C-terminal truncation was further divided into two halves, extending from aa 1177 to 1338 and from 1338 to 1469, the ability to coimmunoprecipitate SNAP45 was lost (Fig. 7B, lanes 10 and 11). We then generated N- and C-terminal truncations of the HA-SNAP190-(1177-1469) fragment. The N-terminal border of the region required for association with SNAP45 is between aa 1281 and 1308 because the HA-SNAP190-(1281-1393) truncation coimmunoprecipitated SNAP45 efficiently, whereas the HA-SNAP190-(1308-1393) truncation coimmunoprecipitated SNAP45 less efficiently (Fig. 7B, compare lanes 9 and 12). The C-terminal border is between aa 1364 and 1393, because the HA-SNAP190-(1281-1393) truncation coimmunoprecipitated SNAP45, whereas the HA-SNAP190-(1281-1364) truncation did not (Fig. 7B, lanes 9 and 13). Thus, the smallest SNAP190 fragment we tested that was still capable of associating fully efficiently with SNAP45 extends from aa 1281 to 1393.
Single and Double aa Changes within the SNAP190 1281-1393 Region
Debilitate Association with SNAP45--
We further defined the SNAP190
sequences required for association with SNAP45 by introducing the
leucine to alanine changes shown in Fig.
8A in SNAP190-(1281-1393) and
testing their effect on association with SNAP45. The results are
summarized in Fig. 8A and shown in Fig. 8, B and
C. Changing leucine 1297 and glutamine 1304 had no effect,
and changing leucines 1301 and 1308 had little effect on association
with SNAP45 (Fig. 8B, compare lanes 10 and 11 with lane 9). However, changing leucine 1314 to alanine severely debilitated association with SNAP45 (lane
14). This is consistent with the analysis of N-terminal SNAP190
truncations above (Fig. 7B, lanes 9 and 12),
which indicates that aa 1281-1308 contribute to efficient association
with SNAP45 but are not absolutely required.
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The C-terminal part of the 1281-1393 SNAP190 region contains 4 leucines (circled in Fig. 8A) separated by 6 aa. However, it is unlikely to form a leucine zipper structure because of the presence of a proline (9), and indeed, the region does not score well when analyzed with the COILS program (20). We mutated three of the leucines that are in register, namely leucine 1355 together with leucine 1362 as well as leucine 1369 to alanines. We also mutated leucine 1364, which is out of register, to alanine. Mutation of leucines 1355 together with 1362 as well as mutation of the out of register leucine 1364 reduced association with SNAP45 to undetectable levels (Fig. 8C, compare lanes 6 and 7 with lane 5). In contrast, mutation of leucine 1369 weakened but did not abolish association with SNAP45 (Fig. 8B, lane 13). These data further argue against this region forming a leucine zipper and are consistent with the deletion analysis above (Fig. 7, A and B, lanes 9 and 13) which indicates that the C-terminal border of the SNAP190 region required for interaction with SNAP45 is between aa 1364 and 1393. The mutation analysis suggests that the C-terminal border lies C-terminal of Leu-1369.
SNAPcs Consisting of Little More Than Protein Sequences
Mapped as Required for Subunit-Subunit Interactions Can Be Assembled
and Bind to the PSE--
We have shown before that we can assemble a
subcomplex of SNAPc, which we call mini-SNAPc
and which consists of SNAP190 sequences extending from aa 1 to 514, full-length SNAP43, and full-length SNAP50. Although in
coimmunoprecipitations of in vitro translated proteins,
SNAP19 is required for detectable association of SNAP190 and SNAP43,
SNAP19 is dispensable for the association of mini-SNAPc in
insect cells, perhaps because a high SNAPc subunit
concentration can be achieved in baculovirus-infected cells (10).
Mini-SNAPc is capable of binding to the PSE and of
directing basal RNA polymerase II and III transcription of snRNA genes
(10). The analysis above identifies several regions within the first
514 aa of SNAP190 and within SNAP43 that are dispensable for
subunit-subunit interactions in the coimmunoprecipitation assay. We
therefore asked whether complexes capable of binding to the PSE could
be assembled from Escherichia coli overexpressed subunits
lacking these regions. The compositions of the various subcomplexes we
tested are shown in Fig. 9A
and an electromobility shift assay (EMSA) performed with probes
containing either the high affinity wild type PSE derived from the
mouse U6 gene (7) or a mutant PSE is shown in Fig. 9B.
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Mini-SNAPc assembled in baculovirus-infected insect cells bound specifically to the PSE, as observed before (10) (Fig. 9B, lanes 2, 3, and 17). Furthermore, complex 1, which is nearly identical to mini-SNAPc except for a slightly shorter SNAP190 truncation (ending at aa 505 instead of 514 in mini-SNAPc), also bound specifically to the PSE (lanes 4, 5, and 18). Complex 1 formed a slightly faster migrating DNA-protein complex in the EMSA than mini-SNAPc expressed in insect cells. This may due to the small difference in size of the SNAP190 truncation or to differences in post-translational protein modifications. Complex 2 contains a truncated SNAP43 lacking the region (aa 269-368) dispensable for association with both SNAP190 and SNAP50 in coimmunoprecipitations (see Fig. 6A). It also bound efficiently to the PSE, although surprisingly, it formed a slower migrating DNA-protein complex than complex 1, even though it is smaller. Perhaps deletion of part of SNAP43 changes the conformation of the complex.
Complexes 4, 6, and 8 contain the same truncated SNAP43 as complex 2 and SNAP190 truncations lacking increasing amounts of N-terminal
sequences up to aa 84. These SNAP190 N-terminal sequences are not
required for association with SNAP43 and SNAP19 in the coimmunoprecipitation assay (see Fig. 2). All these complexes could
bind specifically to the PSE (lanes 6-13 and
19-22). Complex 15 contains full-length SNAP50, SNAP43,
SNAP19, and a SNAP190 truncation lacking aa 1-84 as well as the region
from aa 134 to 262, which separates the domain required for association
with SNAP43 and SNAP19 (aa 84-133, see Fig. 2) from the Myb repeat domain (aa 263-503). This complex could bind specifically to the PSE,
albeit not as efficiently as the other subcomplexes (lanes 14, 15, and 23). The same complex assembled in the absence
of SNAP19 did not generate a DNA-protein complex in the EMSA (data not
shown), suggesting that, with this particular SNAP190 truncation, either association with SNAP43 is not efficient enough in the absence
of SNAP19 for assembly of the complex or SNAP19 is required for DNA
binding. Importantly, however, these results show that DNA-binding
complexes can be assembled from truncated subunits lacking sequences
dispensable for coimmunoprecipitation of individual subunits. This
observation strongly suggests that the protein-protein contacts mapped
by the coimmunoprecipitation assay do indeed occur within
SNAPc.
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DISCUSSION |
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We have mapped protein regions required for subunit-subunit association within SNAPc. The assay we have used, coimmunoprecipitation of polypeptides translated in vitro, is a stringent assay that probably does not detect weak interactions. Thus the interactions we have mapped are probably the strongest but not necessarily the only protein-protein interactions within SNAPc.
Fig. 10 summarizes the functional
domains mapped in this and previous work in the various
SNAPc subunits. Within SNAP190, aa 84 to 133 are sufficient
for association with SNAP19 alone and with SNAP43 together with SNAP19.
This SNAP190 region, and the N-terminal part of SNAP19, are likely to
form -helices and may be involved in a coiled-coil type of
interaction with each other. Indeed, SNAP19 contains five leucines
spaced by 6 aa, and mutations of these leucines have a strong negative
effect, whereas mutation of a leucine out of register has little effect
(Fig. 5). Similarly, SNAP190 contains six leucines and glutamines that
are separated by 6 aa and are, therefore, predicted to reside on the
same face of an
-helix. Mutations that change subsets of these aa
debilitate association with SNAP19 and SNAP43 together with SNAP19,
whereas a double mutation that changes 2 aa predicted to reside on
another face of the helix, one of which is a leucine, has a much weaker negative effect on these associations (Fig. 4). Interestingly, changing
in register glutamines to leucines (mutations Q94L and Q115L) was as
debilitating as changing them to alanines (see Fig. 4A).
Thus, although this SNAP190 region is likely to form an
-helix involved in a coiled-coil type of interaction, it does not correspond to a classical leucine zipper. In SNAP43, aa 164-268 are sufficient for association with SNAP190 and SNAP19 and aa 1-163 are sufficient for association with SNAP50. Thus, these two association domains in
SNAP43 are completely separable.
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C-terminal to the SNAP19/SNAP43 association domain, SNAP190 contains an
unusual Myb domain extending from aa 263 to 503, with four and a half
Myb repeats designated the Rh (for R half), Ra, Rb, Rc, and Rd repeats
(9). The last two Myb repeats (Rc and Rd), but not the first two and a
half (Rh, Ra, and Rb), are required for binding to the PSE (10). Within
the C-terminal half of SNAP190 are two additional regions involved in
protein-protein contacts. The Oct-1 interaction domain (OIR) lies
between aa 869 and 912 (13), and the region required for interaction
with SNAP45 lies between aa 1281 and 1393. Thus, the SNAP190 regions
required for association with SNAP19/SNAP43 and with SNAP45 lie at
opposite ends of the linear molecule. In our original description of
SNAP190, we pointed out that the region defined here as required for
association with SNAP45 contains leucine residues spaced by 6 aa, but
we stressed that the leucines were unlikely to form a leucine zipper
because of the presence of a proline (9). Indeed, our mutagenesis of this SNAP190 region does not support a model in which this region would
form an -helix with one face of the helix involved in
protein-protein contacts with SNAP45 (Fig. 8).
The protein-protein contacts described above were defined in an assay in which we tested the abilities of two or three SNAPc subunits to coimmunoprecipitate. The observation that we can assemble subcomplexes of SNAPc with subunits lacking many of the regions dispensable in the coimmunoprecipitation assay (see Fig. 9) strongly suggests, however, that the protein-protein contacts mapped in the coimmunoprecipitation assay reflect protein-protein contacts that indeed occur within SNAPc. The assembly of subcomplexes also provides some additional information. In our previous work, we assembled mini-SNAPc, a complex missing SNAP19, SNAP45, and the last two-thirds of SNAP190 (10). However, because in the coimmunoprecipitation assay, SNAP43 does not associate efficiently with SNAP190 in the absence of SNAP19, and because mini-SNAPc was assembled from recombinant subunits overexpressed in insect cells, we could not exclude the possibility that a SNAP19 endogenous to insect cells was getting incorporated into mini-SNAPc. Here we show that subcomplexes assembled from subunits expressed in E. coli and missing SNAP19 can also be assembled and bind DNA. This confirms that SNAP19 is not absolutely required for assembly of SNAPc. SNAP19 probably has a stabilizing role, however, because when we used a SNAP190 subunit consisting only of aa 84-133 and 263-518, we observed DNA binding only when SNAP19 was present in addition to SNAP43 and SNAP50.
Neither the C-terminal two-thirds of SNAP190 nor SNAP45 are required
for basal RNA polymerase II and III transcription of snRNA genes (10),
suggesting that this region of the complex has a regulatory role.
Indeed, this region is involved in down-regulation of SNAPc
binding to DNA because deletion of this entire region results in a
complex that binds DNA much more efficiently than complete
SNAPc (10). This region is also required for cooperative binding with the Oct-1 POU domain and contains the OIR, a small SNAP190
region sufficient for association with Oct-1 in an electrophoretic mobility shift assay (13). It is possible that a region within the
C-terminal two-thirds of SNAP190 and/or SNAP45 normally mask the DNA
binding domain of SNAPc but undergo a conformational change upon binding of the OIR to the Oct-1 POU domain. It will be interesting to determine whether the C-terminal two-thirds of SNAP190 and/or SNAP45
are capable of associating with mini-SNAPc or derivatives thereof in trans. This work provides a detailed map of
subunit-subunit contacts within a multisubunit complex involved in both
RNA polymerase II and III basal transcription and opens the way to
determining the functions of SNAPc subunit domains not
required for subunit-subunit interactions.
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ACKNOWLEDGEMENTS |
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We thank R. W. Henry for help and suggestions during the course of this work. We also thank P. S. Pendergrast and A. Saxena for comments on the manuscript and M. Ockler, J. Duffy, and P. Renna for artwork and photography.
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FOOTNOTES |
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* This work was supported in part by National Institutes of Health Grants GM38810 (to N. H.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Supported by the Howard Hughes Medical Institute.
§ To whom correspondence should be addressed: Cold Spring Harbor Laboratory, 1 Bungtown Rd., Cold Spring Harbor, NY 11724. Tel.: 516-367-8362; Fax: 516-367-6801; E-mail: Hernande@cshl.org.
Published, JBC Papers in Press, October 30, 2000, DOI 10.1074/jbc.M009301200
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ABBREVIATIONS |
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The abbreviations used are: snRNA, small nuclear RNA; aa, amino acids; PSE, proximal sequence element; TBP, TATA-binding protein; HA, hemagglutinin; EMSA, electromobility shift assay; OIR, Oct-1 interacting region.
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