Mutational Analysis of the Fractalkine Chemokine Domain

BASIC AMINO ACID RESIDUES DIFFERENTIALLY CONTRIBUTE TO CX3CR1 BINDING, SIGNALING, AND CELL ADHESION*

Jeffrey K. HarrisonDagger §, Alan M. Fong, Peter A. W. SwainDagger , Shuzhen ChenDagger , Yen-Rei A. Yu||, Mina N. SalafrancaDagger , William B. GreenleafDagger , Toshio Imai**, and Dhavalkumar D. Patel||DaggerDagger

From the Dagger  Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, Florida 32610-0267, the Departments of  Medicine and || Immunology, Duke University Medical Center, Durham, North Carolina 27710, and ** KAN Research Institute, Kyoto 600-8815, Japan

Received for publication, November 10, 2000, and in revised form, March 5, 2001


    ABSTRACT
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Fractalkine (FKN/CX3CL1) is a unique member of the chemokine gene family and contains a chemokine domain (CD), a mucin-like stalk, a single transmembrane region, and a short intracellular C terminus. This structural distinction affords FKN the property of mediating capture and firm adhesion of FKN receptor (CX3CR1)-expressing cells under physiological flow conditions. Shed forms of FKN also exist, and these promote chemotaxis of CX3CR1-expressing leukocytes. The goal of the present study was to identify specific residues within the FKN-CD critical for FKN-CX3CR1 interactions. Two residues were identified in the FKN-CD, namely Lys-7 and Arg-47, that are important determinants in mediating an FKN-CX3CR1 interaction. FKN-K7A and FKN-R47A mutants exhibited 30-60-fold decreases in affinity for CX3CR1 and failed to arrest efficiently CX3CR1-expressing cells under physiological flow conditions. However, these mutants had differential effects on chemotaxis of CX3CR1-expressing cells. The FKN-K7A mutant acted as an equipotent partial agonist, whereas the FKN-R47A mutant had marked decreased potency and efficacy in measures of chemotactic activity. These data identify specific structural features of the FKN-CD that are important in interactions with CX3CR1 including steady state binding, signaling, and firm adhesion of CX3CR1-expressing cells.


    INTRODUCTION
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Leukocyte trafficking is a complex physiological process that is dependent upon a number of cellular events that include mechanisms of cell chemotaxis and adhesion (1, 2). In this regard, chemokines are a growing family of small molecular mass proteins whose role(s) are primarily involved in leukocyte migration (3). Fractalkine (FKN)1 is a newly described member of the chemokine gene family and the only known member of the CX3C subfamily (4, 5). The extended structure of FKN is distinct among the chemokine gene family in that it affords the molecule unique membrane localization where it can mediate FKN receptor, CX3CR1, -dependent cell adhesion (6-8). FKN is expressed primarily by neurons (9-11) and endothelial cells (6, 12). Neuronally expressed FKN is presumably involved in the central nervous system-based communication with CX3CR1-expressing microglia, whereas FKN expressed on endothelial cells likely mediates extravasation of CX3CR1-expressing cells, which include monocytes, T cells, and NK cells, out of the lumen of the blood vessel into the tissue parenchyma. FKN is also expressed by dendritic cells and intestinal epithelial cells, and CX3CR1 has recently been demonstrated on mast cells (13-16).

The dissection of molecular processes involving chemokines and chemokine receptors is an active area of investigation as development of therapeutic agents targeting these systems are likely to yield novel beneficial approaches in the treatment of inflammation, cancer, as well as inhibition of viral (human immunodeficiency virus) pathogenesis. Given the localization of FKN to endothelial, dendritic, and neuronal cells, this molecule also constitutes a potential therapeutic target for modulating several physiological processes including leukocyte trafficking and/or mechanisms involved neuroinflammation. The goal of the present study was aimed at identifying amino acid residues in the FKN molecule that are important determinants for binding to, and activating its receptor, CX3CR1. We employed deletional and site-directed mutagenesis in order to evaluate the effect of different lengths of the mucin stalk and determine the role of specific amino acid residues present in the FKN chemokine domain, in mediating FKN binding, signaling, and CX3CR1-dependent cell adhesion.

    EXPERIMENTAL PROCEDURES
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Mutagenesis-- DNA sequences encoding human fractalkine were cloned to the EcoRI/XhoI site of pcDNA3 (huFKN/pcDNA3). Five truncation mutants of FKN were prepared by site-directed mutagenesis using huFKN/pcDNA3 as the template and five paired sets of complementary oligonucleotides encoding a different region of the mucin stalk (see Fig. 1). The oligonucleotides used are as follows A, 5'-CGA GTC TGT GGT CCT CGA GCC CGA AGC CAC AGG-3' and 5'-CCT GTG GCT TCG GGC TCG AGG ACC ACA GAC TCG-3'; B, 5'-CGC CAA AGG CTC AGG CTC GAG GGC CTG TGG GCA CGG-3' and 5'-CCG TGC CCA CAG GCC CTC GAG CCT GAG CCT TTG GCG-3'; C, 5'-GGA GAA TGC TCC GTC TCG AGG CCA GCG TGT GTG G-3' and 5'-CCA CAC ACG CTG GCC TCG AGA CGG AGC ATT CTC C-3'; D, 5'-CCA TGC CAC CAT GGA CCT CGA GAG GCT GGG CGT CC-3' and 5'-GGA CGC CCA GCC TCT CGA GGT CCA TGG TGG CAT GG-3'; and E, 5'-CGC CCA GGC TGC CAC CTC GAG GCA GGC GGT GGG GC-3' and 5'-GCC CCA CCG CCT GCC TCG AGG TGG CAG CCT GGG CG-3'. Each mutation introduced an additional XhoI restriction endonuclease recognition sequence into the plasmid. Mutant DNA was generated using the PCR; the original template was excised by subsequent DpnI treatment. Plasmid DNA was isolated from random ampicillin-resistant bacterial colonies and subjected to restriction endonuclease digestion analysis using XhoI. The entire protein coding sequence of each mutant plasmid was subject to DNA sequence analysis in order to confirm the mutation and the fidelity of the DNA polymerase (Pfu) used in the PCR. An EcoRI/XhoI fragment of each truncation mutant was then subcloned to the same site(s) of pcDNA3.1/myc/His6 (Invitrogen); the specific version used depended on the frame in which the XhoI site was engineered. DNA sequence analysis of the final plasmid constructs was also performed in order to verify that the nucleotides encoding the myc/His6 were in frame with the nucleotides encoding the FKN.

Site-directed mutagenesis of the chemokine domain of FKN (FKN-CD) was also carried out using oligonucleotide-directed PCR and subsequent DpnI treatment. Mutants were generated in the huFKN/pcDNA3 (full length) and the truncation mutant, form E (described above). The specific oligonucleotide pairs used for the mutagenesis are as follows (see Fig. 1 in reference to the specific residues mutated): K7A, 5'-GCA CCA CGG TGT GAC GGC ATG CAA CAT CAC GTG C-3' and 5'-GCA CGT GAT GTT GCA TGC CGT CAC ACC GTG GTG C-3'; K14A, 5'-GCA ACA TCA CGT GCA GCG CGA TGA CAT CAA AGA TAC C-3' and 5'-GGT ATC TTT GAT GTC ATC GCG CTG CAC GTG ATG TTG C-3'; M15K, 5'-GCA ACA TCA CGT GCA AGA AGA CAT CAA-3' and 5'-GGT ATC TTT GAT GTC TTC TTG CTG CAC GTG ATG TTG C-3'; K36A, 5'-CCA GGC ATC ATG CGG CGC ACG CGC AAT CAT CTT GG-3' and 5'-CCA AGA TGA TTG CGC GTG CGC CGC ATG ATG CCT GG-3'; R37A, 5'-CCA GGC ATC ATG CGG CAA AGC CGC AAT CAT CTT GGA G-3' and 5'-CTC CAA GAT GAT TGC GGC TTT GCC GCA TGA TGC CTG G-3'; R47A, 5'-GGA GAC GAG ACA GCA CGC GCT GTT CTG TGC CGA C-3' and 5'-GTC GGC ACA GAA CAG CGC GTG CTG TCT CGT CTC C-3'; K54A, 5'-GTT CTG TGC CGA CCC GGC GGA GCA ATG GGT CAA GG-3' and 5'-CCT TGA CCC ATT GCT CCG CCG GGT CGG CAC AGA AC-3'; R74A, 5'-CTG CTG CCC TAA CTG CAA ATG GCG GCA CCT TCG-3' and 5'-CGA AGG TGC CGC CAT TTG CAG TTA GGG CAG CAG-3'; and NIT to EIM, 5'-CAC CAC GGT GTG ACG AAA TGC GAG ATC ATG TGC AGC AAG ATG ACA TC-3' and 5'-GAT GTC ATC TTG CTG CAC ATG ATC TCG CAT TTC GTC ACA CCG TGG TG-3.

Expression of FKNs in HEK293T Cells-- Approximately 10 million HEK293T cells (seeded in two 100-mm dishes) were transfected using 10 µg of plasmid DNA/plate and LipofectAMINE according to the manufacturer's instructions. Cells were incubated in DMEM containing 10% FBS for the first 20 h post-transfection and subsequently incubated with DMEM containing 0.5% FBS for an additional 24 h. Media were harvested and subjected to Ni-NTA chromatography according to the manufacturer's (Qiagen QIAexpressionist 03/99, Valencia, CA) recommended procedures. Briefly, conditioned media were mixed with an equal volume of 2× buffer (100 mM NaH2PO4, pH 8.0, 600 mM NaCl, 20 mM imidazole, pH 8.0) and 1 ml of the Ni-NTA slurry. The mixture was rocked for 1 h at 4 °C. After rocking, the mixture was loaded into a filtration column, and the packed resin bed was subsequently washed with 8 ml of wash buffer (50 mM NaH2PO4, pH 8.0, 300 mM NaCl, 20 mM imidazole, pH 8.0). The protein was eluted with elution buffer (4 × 0.5 ml aliquots, 50 mM NaH2PO4, pH 8.0, 300 mM NaCl, 250 mM imidazole, pH 8.0), flash-frozen in liquid N2 in 100-µl aliquots, and stored at -80 °C until further use.

Western Blot Analysis-- Aliquots of conditioned media, Ni-NTA column flow-through, column washes, and the elutions were subjected to SDS-PAGE and Western blot analysis analysis as described previously (9). Aliquots were electrophoresed through 10% polyacrylamide gels. The proteins were transferred onto a polyvinylidene difluoride 0.45-µm membrane (Millipore, Bedford, MA) and blocked on a rotating table for 0.5 h in TBS-T (Tris-buffered saline/Tween), 5% milk (20 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.4). After drying, the membranes were incubated for 2 h with a rabbit anti-fractalkine antibody (1:10,000 dilution) in TBS-T, 5% nonfat instant milk. The membranes were washed 3 times (10 min per wash) in TBS-T and subsequently incubated in a goat anti-rabbit IgG/horseradish peroxidase (1:10,000) in TBS-T, 5% milk for 1 h. The membranes were washed 3 times (10 min per wash) in TBS-T and then developed for 5 min with Pierce Supersignal chemiluminescent substrate (Pierce). Membranes were finally exposed onto Amersham ECL film (Amersham Pharmacia Biotech).

Whole Cell Radioligand Binding Analysis-- Approximately 50,000 CHO cells stably expressing human or rat CX3CR1, or 300,000 rat microglia were seeded into the wells of a 12-well plate. Two days later, whole cell radioligand binding analysis was performed according to procedures published previously (9). Briefly, cells were rinsed (1 time) with PBS and subsequently incubated for 1 h in 0.5 ml of HBSS containing 10 mM Hepes-Na, pH 7.4, and 0.1% BSA (binding buffer) with 0.5-1 nM 125I-huFKN-CD (specific activity, 200-300 Ci/mmol) in the absence and presence of various concentrations of unlabeled inhibitors (FKN-CD, truncation mutants, or FKN-CD mutants). At the end of the incubation cells were rinsed 3 times (1 ml per wash) with ice-cold binding buffer. Radioactivity retained in the wells was extracted with 0.2 N NaOH and subject to gamma spectroscopy. Nonspecific binding was determined in the presence of 100 nM huFKN-CD. Data are expressed as percent of total specific binding. Graphical and statistical analysis was carried out using Prism 2.0 (GraphPad Software, Inc., San Diego, CA).

Measurement of Intracellular Calcium Levels-- Intracellular calcium levels in fura-2 AM-loaded CHO-huCX3CR1 cells were assayed as described previously (9) using a spectrofluorimeter (Photon Technology International, Monmouth Junction, NJ). Cells were illuminated alternately with ultraviolet light (1/s) of 340 and 380 nm wavelength and monitored for fluorescence at 510 nm. Due to the uncertainties involved in the calculation of [Ca2+]i, the signals are reported as changes in F340/F380, which gives a relative measure of [Ca2+]i.

FKN-ELISA-- The concentrations of purified soluble FKN proteins were determined by ELISA using anti-FKN antibodies. One hundred µl/well of 2 µg/ml capture anti-FKN (R & D Systems, Minneapolis, MN) was incubated overnight at room temperature in a 96-well plate. After washing three times in wash buffer (0.05% Tween 20 in PBS), the wells were blocked with 1% BSA, 5% sucrose, 0.05% NaN3 for 1 h. Following three more washes, 100 µl of each diluted protein sample was added and incubated for 1 h at 37 °C. After 3 washes, 100 µl of 100 ng/ml biotinylated anti-FKN (R & D Systems) diluted in PBS containing 0.05% Tween 20, 0.05% NaN3, 0.2% BSA was added and incubated for 1 h at 37 °C. The plates were then washed 3 times, and 100 µl of 1:4000 dilution streptavidin horseradish peroxidase (Zymed Laboratories Inc., South San Francisco, CA) was added and incubated for 20 min at room temperature. After 3 washes, horseradish peroxidase activity was detected colorimetrically using the TMB microwell peroxidase substrate system (Kirkegaard & Perry, Gaithersburg, MD) and the results read on a Dynex microplate reader. Protein concentrations were determined by averaging the results of three independent ELISA assays.

Chemotaxis Assays-- Chemotaxis toward soluble FKN and FKN-CD mutants was addressed by migration through transwells. Various dilutions of Ni-NTA-eluted fractions containing wild type and mutant proteins were made in 600 µl of medium (RPMI 1640 containing 10% FBS) and placed in the bottom of a transwell (6.5 mm diameter, 3-µm pore size, Costar, Corning, NY). One million L1.2 cells expressing CX3CR1-GFP were resuspended in 100 µl of RPMI medium and placed into the top chamber of a transwell. After 4 h of incubation at 37 °C, the bottom well was harvested. The number of migrating cells was determined by counting a 20-µl aliquot using flow cytometry (Coulter Epics XL-MCL). Ni-NTA column elution buffer had no inhibitory or enhancing effects on L1.2 cell chemotaxis. For each experiment, values were normalized to wild type FKN with maximal chemotaxis to wild type FKN set at 100%. Concentration-response curves were generated by curve fit interpolation analysis using Cricket Graph III (Computer Associates International, Islandia, NY). Statistical analysis was carried out using Statistica 3.0b (Statsoft Inc.)

Expression of FKNs in EA.hy 926 Cells-- EA.hy 926 cells were grown in DMEM containing 10% FBS. Cells (grown to 80% confluence) were transfected with 10 µg of DNA using a calcium phosphate precipitation-based method. Forty eight hours post-transfection, cells were seeded into media containing 0.5 mg/ml G418. G418-resistant cells were analyzed for cell surface levels of fractalkine by staining with a monoclonal antibody specific for the mucin domain of fractalkine (mAb 1D6). Results were visualized on a Coulter Epics-XL flow cytometer.

Capture and Firm Adhesion under Physiological Flow-- Firm adhesion of CX3CR1-expressing cells was assessed by a parallel plate flow chamber assay as described previously (7). Briefly, EA.hy 926 cells were plated onto glass coverslips and allowed to grow overnight at 37 °C. The coverslips were then placed in a parallel plate flow chamber. K562 or K562-CX3CR1 cells were resuspended in flow buffer (PBS containing 0.75 mM CaCl2, 0.75 mM MgCl2, 0.5% BSA) at a concentration of 1 × 106 cells/ml. The cells were perfused at a shear stress of 0.25 dynes/cm2 for 5 min. Following subsequent 1 min washes of 0.5, 1, and 1.85 dynes/cm2 for 1 min each, the cells were subjected to a high shear stress of 5 dynes/cm2 in flow buffer. The results of each run were recorded onto videotape. The number of K562 cells remaining bound to the monolayer was then counted. The number of captured cells on EA.hy 926 cell transfectants was determined by counting the number of cells that were in contact with the monolayer for at least 5 s during the initial loading phase in the flow chamber (shear stress of 0.25 dynes/cm2). For all monolayers, greater than 90% of the captured cells remained firmly adherent (n = 3).

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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
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Although FKN is expressed as a membrane-attached protein, extracellular forms of this protein are readily detected. The specific cleavage site(s) that generate these shed forms of FKN are not known. FKN released from the cell surface has an observed molecular mass greater than predicted and thus is likely to contain all or a portion of the glycosylated mucin-like stalk. Our first goal was directed toward determining the effect of various lengths of this mucin-like domain on the affinity and efficacy of the FKN-CD for binding and activating CX3CR1. Fig. 1 depicts the entire protein sequence of human FKN, in alignment with the chemokine domains of the two known rodent orthologs (rat and murine FKN-CD). In order to evaluate the effect of various lengths of the mucin stalk on FKN-CD function, five truncation mutants of human FKN (designated A-E) were prepared in which DNA sequences encoding the signal peptide, the chemokine domain, and differential lengths of the mucin stalk were cloned in frame with C-terminal six histidine residues (His6). The C-terminal addition of the His6 residues facilitated purification of the various secreted FKN truncation mutants from the conditioned media of the HEK293T cell transfectants (using Ni-NTA chromatography). In addition, the figure highlights conserved lysine and arginine residues, present in the chemokine domain of the various fractalkine orthologs, that were targeted for site-directed mutagenesis (to alanine). Since chemokine receptors typically contain a number of aspartate and glutamate residues within their extracellular regions, these negatively charged side chains present in the receptor are likely candidate amino acids for interaction with positively charged amino acids that are prevalent not only in FKN but also in other chemokine sequences. Thus, we hypothesized that specific basic residues present within the FKN molecule are critical for interactions with its receptor CX3CR1. In addition, a single methionine residue (at position 15) and a unique N-linked glycosylation sequence (NIT) present in human and rat FKN are highlighted. Met-15 was previously suggested to interact with a peptide containing the sequences of the N terminus of human CX3CR1 (17); this residue was mutated to a lysine. From structural studies, the single N-linked glycosylation in the human FKN sequence, which is also present in the cognate region of rat FKN, appears to be a readily available substrate for this post-translational modification. Thus, in order to assess the role of this putative glycosylation site in the interaction of FKN with CX3CR1, we mutated the N-linked glycosylation sequence to "EIM"; this tri-amino acid motif is EIM in the murine ortholog.


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Fig. 1.   Alignment of human fractalkine (huFKN) with rat and murine fractalkine chemokine domains (ratFKN-CD and murFKN-CD). Basic residues conserved within the three FKN orthologs (Lys-7, Lys-14, Lys-36, Arg-37, Arg-47, Lys-54, and Arg-74) were changed to alanine and are indicated in boldface. Boldface methionine (Met-15) was mutated to a lysine. The unique N-linked glycosylation consensus sequence (NIT) present in human and rat FKN-CDs is underlined. The starred residues denote the four conserved cysteine residues; the boldface and underlined sequences correspond to the transmembrane spanning domain.

The effect of the length of the mucin stalk on the binding of the FKN-CD to CX3CR1 and the stimulation of intracellular calcium mobilization in CX3CR1-expressing cells were assessed. Western blot analysis (Fig. 2A) of the five 293T cell-expressed truncation mutants of FKN, purified by Ni-NTA chromatography, indicated that all five forms were expressed and secreted into the HEK293T cell-conditioned media. All of the secreted proteins migrated as broad, diffuse bands thru SDS-PAGE at molecular masses greater than their calculated molecular masses based upon the predicted protein backbones (Table I). This is likely due to the glycosylated characteristics of the mucin stalk. Nevertheless, the relative sizes of the various truncation mutants were consistent with the protein containing the CD and progressively increasing lengths of the mucin stalk. Competition binding analysis (125I-FKN-CD binding to huCX3CR1-CHO cells) of each of these truncation mutants indicated that each form displayed a comparable affinity for human CX3CR1 (Fig. 2B). Furthermore, each form of the protein stimulated similar increases in intracellular calcium levels in the fura-2-loaded huCX3CR1-CHO cells (Fig. 2C). Collectively the data indicate that the mucin stalk does not contribute to FKN binding affinity and efficacy for stimulating calcium mobilization in CX3CR1-expressing cells.


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Fig. 2.   Binding and activation of CX3CR1 by huFKN-CD is not modulated by the length of the mucin stalk. Truncated forms of human FKN were prepared as indicated under "Experimental Procedures." A, Western blot analysis of huFKN-CD with various lengths of the mucin stalk. 293T cells were transfected with plasmid DNA encoding five different forms of huFKN (A-E, as designated in Fig. 1). Myc/His6-tagged proteins present in the conditioned media 2 days post-transfection were purified using Ni-NTA chromatography, and an aliquot (1 µl) of one of the elution fractions was subject to SDS-PAGE and Western blot analysis using the anti-hufractalkine antibody according to procedures indicated under "Experimental Procedures." The 1st lane contains 2.5 pmol of human FKN chemokine domain (CD) purchased from R & D Systems. Numbers to the left denote relative molecular mass of protein standards. B, competition binding analysis of huFKN truncation mutants. Radioligand binding analysis of 125I-huFKN-CD binding to huCX3CR1-CHO cells. Whole cell binding analysis of 125I-huFKN-CD in the absence and presence of increasing amounts of the NI-NTA-eluted protein was performed according to procedures outlined under "Experimental Procedures." Data are plotted as % Bound as a function of volume of the eluate added. Results are expressed as mean ± S.E. from three independently conducted experiments performed in triplicate. Inset depicts competition by huFKN-CD. C, stimulation of intracellular calcium mobilization by huFKN truncation mutants. Representative traces from a single experiment are depicted. Results are representative of three independently conducted experiments.

                              
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Table I
Predicted and observed molecular masses of FKN truncation mutants
Shown are the calculated molecular masses (in kilodaltons) of the predicted protein backbones of the various FKN truncation mutants as well as the molecular mass ranges of the expressed proteins as observed by SDS-PAGE and Western blot analysis (depicted in Fig. 2). The predicted molecular masses exclude the mass contributed by the putative signal peptide sequence.

The effect of mutating the species conserved basic residues, the single N-linked glycosylation consensus sequence, and Met-15, in the FKN-CD, on the binding of FKN to CX3CR1 was evaluated. Site-directed mutants of these residues were prepared in the context of the longest secreted truncation mutant (CD with all of the mucin stalk, i.e. form E) and purified by Ni-NTA chromatography. Western blot analysis (Fig. 3A) indicated that all of the Ni-NTA-purified mutants were present at concentrations equivalent to the levels of the purified "wild type" form. Ni-NTA-purified proteins were then evaluated for their relative potency for inhibiting the binding of 125I-FKN-CD to the huCX3CR1-CHO cells. The K7A and R47A mutants displayed significantly lower binding potencies than the wild type and all other mutant forms (Fig. 3B). Estimates of the relative shift in potencies of these two forms were ~30-60-fold. FKN-CD mutants showing comparable binding affinities for CX3CR1 were evaluated for their ability to stimulate increases in intracellular calcium in fura-2-loaded huCX3CR1-CHO cells. At concentrations that achieve near full receptor occupancy (~90%), each of these FKN mutants stimulated increases in intracellular calcium that were comparable to the "wild type" FKN (Fig. 3C). At concentrations equivalent to ~75% receptor occupancy by the wild type FKN, FKN-K7A, and -R47A mutants stimulated lower increases in intracellular calcium that correlated with the decreased binding affinity of these mutants.


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Fig. 3.   Lysine 7 and arginine 47 are critical amino acids in the binding of FKN to human CX3CR1. Western blot and binding analysis of huFKN-CD mutants. Site-directed mutagenesis of conserved basic residues within the huFKN-CD was performed according to procedures indicated under "Experimental Procedures." Plasmid DNA encoding the longest truncation mutant (CD with all of the mucin stalk) served as the template in the mutagenesis reactions. Proteins were expressed in 293T cells and purified by Ni-NTA chromatography. A, Western blot analysis of wild type (WT) and CD mutants (K7A, K14A, M15K, K36A, R37A, R47A, K54A, R74A, NIT to EIM) was performed as described above. The equivalent of 0.25 µl of each mutant were electrophoresed. The numbers below each lane indicate the site of the specific mutation. B, competition binding analysis of huFKN-CD mutants. Radioligand binding analysis of 125I-huFKN-CD binding to huCX3CR1-CHO cells is shown. Whole cell binding analysis of 125I-huFKN-CD in the absence and presence of increasing amounts of the Ni-NTA-eluted protein was performed according to procedures indicated above. Data are plotted as % Bound as a function of volume of the eluate of a single fraction added. Results are expressed as mean ± S.E. from three independently conducted experiments performed in triplicate. WT (black-triangle), K7A (black-down-triangle ), K14A (black-diamond ), M15K (), K36A (), R37A (triangle ), R47A (down-triangle), K54A (diamond ), R74A (open circle ), NIT to EIM (×). C, stimulation of intracellular calcium mobilization by huFKN-CD mutants. Representative traces from a single experiment are depicted. Results are representative of three independently conducted experiments.

The concentration-dependent effect of FKN to promote chemotaxis of CX3CR1-expressing cells was evaluated for each of the mutants (Fig. 4). FKN-K7A and -R47A mutants displayed reduced chemotactic activity relative to that observed with wild type FKN. The FKN-R47A mutant was nearly ineffective at stimulating a chemotactic response, with maximal activity less than 10% of the wild type activity, whereas the FKN-K7A mutant retained ~60% of the wild type maximal activity. The FKN-K36A mutant had slightly enhanced maximal chemotactic activity. Mutation of Met-15 (to Lys) yielded an FKN that was ~10-fold less potent at promoting chemotaxis; these data are consistent with the slight reduction in binding affinity of FKN-M15K (see Fig. 3B). Table II summarizes the effect of the mutations on the relative concentration dependence and maximal chemotactic activities of the mutant FKNs relative to the respective wild type FKN parameters.


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Fig. 4.   Chemotaxis of CX3CR1-GFP-expressing L1.2 cells to FKN-CD mutants. Chemotaxis toward different concentrations of soluble FKN mutant proteins was tested as described under "Experimental Procedures." Mutant FKN protein concentrations were determined by ELISA. Shown are the concentration-response curves for mutant FKN proteins (filled circles with solid lines) compared with wild type FKN (open circles with dashed lines). Shown are the means ± S.E. from three independent experiments. The numbers of migrating cells were normalized to wild type FKN with 100% representing the peak chemotaxis to wild type FKN in each experiment.

                              
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Table II
Relative EC50 values and maximal chemotaxis of FKN-CD mutants
Shown are EC50 values and maximal chemotactic activity for FKN-CD mutants, relative to EC50 and maximal response of wild type (WT) FKN at stimulating chemotaxis of CX3CR1-L1.2 cells. Results are expressed as means ± S.D. from three independent experiments. ND, not determined.

As stated, the specific basic residues targeted for mutagenesis are conserved in human, rat, and mouse FKNs. Analysis of the relative potencies of the mutants in binding to rat CX3CR1 revealed similar decreased affinities of the K7A and R47A mutants. This was evident regardless if the source of rat CX3CR1 was recombinant (Fig. 5A, rat CX3CR1-CHO cells) or native cell expressed (Fig. 5B, rat microglia). These data indicate similar interactions of the FKN with CX3CR1 in humans and rodents and point to conserved amino acids in these receptor orthologs as the counter amino acid residues interacting with amino acids Lys-7 and Arg-47 in FKN.


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Fig. 5.   Analysis of huFKN-CD mutant binding to rat CX3CR1. A, radioligand binding analysis of 125I-huFKN-CD binding to rat CX3CR1-CHO cells in the presence of huFKN-CD mutants. Whole cell binding analysis of 125I-huFKN-CD in the absence and presence of increasing amounts of the Ni-NTA-eluted protein was performed according to procedures indicated above. Data are plotted as % Bound as a function of volume of the eluate of a single fraction added. Results are expressed as mean ± S.E. from one experiment performed in triplicate. The results are representative of two experiments. WT (black-triangle), K7A (black-down-triangle ), K14A (black-diamond ), M15K (), K36A (), R37A (triangle ), R47A (down-triangle), K54A (diamond ), R74A (open circle ), NIT to EIM (×). B, radioligand binding analysis of 125I-huFKN-CD binding to rat microglial cells in the presence of huFKN-CD mutants. Whole cell binding analysis of 125I-huFKN-CD in the absence and presence of increasing amounts of the Ni-NTA-eluted protein was performed. Data are plotted as % Bound as a function of volume of the eluate of a single fraction added. Results are expressed as mean ± S.E. from one experiment performed in triplicate. The results are representative of two experiments. WT (black-triangle), K7A (black-down-triangle ), R47A (down-triangle).

Parallel mutations were generated in the full-length FKN, in order to assess the role of these residues on FKN-dependent cell adhesion. The endothelial cell line, EA.hy 926, was transfected with each of these mutant constructs, and G418-resistant clonal cells were sorted. EA.hy 926 cells are derived from human umbilical vein endothelial cells and display a number of endothelial cell-like properties. Cell surface expression levels of these mutant FKNs in the stably transfected cells were roughly equivalent in all cell lines generated, as evidenced by flow cytometry (Fig. 6). The ability of CX3CR1-expressing cells to bind to the EA.hy 926 cells expressing the mutant FKNs was determined by a parallel plate flow chamber assay. Fig. 7 shows that K562-CX3CR1 cells efficiently bind to all of the mutant FKN EA.hy 926 cells to the same extent as the wild type FKN-expressing EA.hy 926 cells with the exception of the K7A and R47A mutant FKN-expressing cells. A 60% reduction in both the capture and firm adhesion of K562-CX3CR1 cells to these two mutant FKN-expressing EA.hy 926 cells, as compared with wild type FKN-EA.hy 926 cells, was evident.


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Fig. 6.   Expression analysis of "full-length" huFKN-CD mutants in EA.hy 926 cells. EA.hy 926 cells stably transfected with various full-length FKN-CD mutants were tested for surface expression by flow cytometry. Shown is the expression of FKN as determined using mAb 1D6 (dark histograms) that recognizes the mucin domain. Background staining with the control mAb P3 is indicated by the clear histograms.


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Fig. 7.   CX3CR1-K562 cell capture by and firm adhesion to FKN mutants expressed on EA.hy 926 cells. Left panel, cell capture. Shown are the relative amounts of K562-CX3CR1 cells captured at low shear stress by EA.hy 926 cells expressing the various FKN mutants compared with wild type FKN at a shear stress of 0.25 dynes/cm2. K562-CX3CR1 cells were allowed to interact with confluent EA.hy 926 monolayers under physiological flow conditions using the parallel plate flow chamber. Capture was defined as cell contact with the monolayer for at least 5 s. Right panel, firm adhesion. Shown are the relative amounts of K562-CX3CR1 cells remaining firmly bound to EA.hy 926 monolayers expressing various FKN mutants following high shear stress. After the initial 5-min loading time at 0.25 dynes/cm2, the cells were subjected to increasing shear stresses as outlined under "Experimental Procedures." After 2 min at 10 dynes/cm2, the number of K562-CX3CR1 cells remaining bound to the monolayer was counted. The values for capture and firm adhesion have been normalized with 100% capture and firm adhesion being the number of cells interacting with or firmly adherent to EA.hy 926 monolayers expressing wild type FKN. Shown are the means and standard deviations from three independent experiments. The FKN mutants with a statistically significant (p < 0.05) difference from the wild type protein are indicated with an asterisk.

A chief concern in the analysis of functional properties of site-directed mutants is whether or not the specific mutation alters global protein conformation, as opposed to disrupting a specific protein-protein interaction. Thus, the effect of the various site-specific mutations on global FKN-CD conformation was assessed using a panel of monoclonal antibodies directed against the CD of human FKN. Cell surface reactivity of each of the mAbs was assessed using the EA.hy 926-expressed proteins. Reactivity of each of the mAbs toward the wild type FKN and the K7A, K14A, K54A, R74A, and NIT-EIM FKN mutants were equivalent (Table III). In contrast, the reactivities of mAbs 2A5 and 8C5 were reduced in the M15K, K36A, and R37A mutants. The monoclonal antibody, mAb 1B7, was less reactive toward the M15K and R37A mutants, whereas mAb 4F2 did not react with the R47A mutant. The spatial proximity of Met-15, Lys-36, and Arg-37 (Fig. 8) is consistent with similar reductions in reactivity by mAbs 2A5, 8C5, and 4F2.

                              
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Table III
Epitope mapping of FKN-CD mutants
Shown are the cell surface reactivities of monoclonal antibodies directed to the mucin (1D6) and chemokine domains of FKN. Cell surface reactivity with FKN mutants was determined by indirect immunofluorescence and flow cytometry. Reactivity was considered negative (-) if the mean fluorescence was less than 20% of maximal.


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Fig. 8.   Model of the FKN-CD depicting the location of the Lys-7 and Arg-47 amino acid residues. The ribbon diagram was generated using the program MOLSCRIPT (25), and the atomic coordinates and structure factors (1f2l) are deposited in the Protein Data Bank, Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, NJ (21). The side chains of Lys-7 and Arg-47 are depicted in red. The side chains of Met-15 and Arg-37 are in blue, and the side chain of Lys-36 is shown in purple. The conserved Cys residues are green. The N and C termini of this structure are marked with N and C, respectively.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The major goal of the current study was aimed at identifying specific basic residues in the chemokine domain of FKN that are critical for receptor interactions. Two amino acid residues, namely Lys-7 and Arg-47, were identified as important amino acid determinants in the binding of FKN to CX3CR1. The significance of these residues in binding interactions was confirmed in two independent methods used to assess binding of FKN to CX3CR1, namely in steady state binding analysis and in in vitro flow chamber assays in which the FKN-CX3CR1 interaction is measured under conditions mimicking physiological blood flow. Whereas the K7A and R47A mutants behaved similarly in assays quantifying binding activities, these mutants had differential effects in measures of signaling via CX3CR1. Although FKN-R47A was essentially ineffective at stimulating intracellular calcium mobilization and chemotaxis of CX3CR1-expressing cells, the FKN-K7A mutant retained signaling activity. Three additional findings were noted in the present study. First, the single N-linked glycosylation site present in human and rat FKN is not glycosylated to any appreciable extent in heterologous expression systems and also does not appear to have any functional significance in the binding or activation of CX3CR1 by FKN. Second, a methionine residue (Met-15), previously suggested to interact with the N terminus of CX3CR1, does not appear to be as critical at interacting with CX3CR1, as expressed in mammalian cells. Third, the affinity and efficacy of the FKN-CD are independent of the length of the mucin stalk.

FKN is a dual function chemokine capable of promoting CX3CR1-expressing cell chemotaxis and cell adhesion. Shed or secreted forms of FKN stimulate leukocyte chemotaxis, whereas the membrane-attached form of FKN mediates cell adhesion (4, 6, 7). A limited level of structure-function analysis of FKN and CX3CR1 has been reported. Although the chemokine domain of FKN is critical for interaction of FKN with CX3CR1, less information is available on the role of the mucin stalk. Under conditions mimicking physiological blood flow, cells expressing "full-length" FKN with the entire mucin domain are more efficient at capturing leukocytes as compared with FKN-CD proteins, i.e. without the mucin stalk (18). Although the arrest of CX3CR1-expressing cells under flow conditions is dependent on the chemokine domain of FKN, the function of the mucin stalk in this setting appears to be as a molecular extender of the FKN-CD to circulating leukocytes. The ability of FKN to arrest cells appears to be a unique property of the FKN chemokine domain as chimeric molecules in which the mucin domain was replaced with a stalk from E-selectin was fully functional in leukocyte arrest (18). Furthermore, chimeric molecules in which other chemokines were "fused" to the FKN mucin stalk failed to arrest cells expressing the cognate chemokine receptor (19).

Given the importance of the chemokine domain of FKN in binding and functional responses of CX3CR1-expressing cells, our studies primarily addressed the roles of specific amino acids in the FKN-CD in interacting with the receptor. A single N-linked glycosylation site is present in the human and rat orthologs of FKN and, according to structural data, is spatially available for post-translational modification (17). Although the human and rat FKN molecules have the potential N-glycosylation site (CNITC), mouse FKN does not contain an N-glycosylation site in the CX3C bulge (CEIMC), indicating that glycosylation may not be important. However, murine FKN does not support arrest of CX3CR1-expressing cells under flow (20). Furthermore, evidence that N-glycosylation may play a role under certain conditions comes from the fact that synthetic, non-glycosylated FKN has diminished activity for monocytes (4). One possibility is that N-glycosylation contributes to monocyte activation and adhesion. In our heterologous expression system, we did not find evidence for glycosylation of this site in the human protein. The relative migration of the NIT to EIM FKN mutant in SDS-PAGE was not significantly different than the mobility of the wild type FKN. Furthermore, these amino acid changes did not affect binding and functional properties, including affinity for the receptor, stimulation of intracellular calcium mobilization, potency, and efficacy in promoting chemotaxis and cell adhesion.

There are six basic residues present within the FKN-CD that are conserved in human, rat, and murine FKN. The similar binding affinities of human and rodent FKN for rat CX3CR1 (9) suggests that conserved residues in FKN are the likely amino acid residues that interact directly with motifs present in the receptor. Mutation of each of these residues to an alanine appeared to have little effect on the global conformation of the FKN-CD. This conclusion is based on two observations from the mAb epitope mapping studies. First, in general the mAbs used in this study reacted as efficiently with the mutants as they did with the wild type FKN. Second, those mutants that showed reduced mAb reactivity (e.g. M15K, K36A, and R37A) did not display significant differences in binding affinities, stimulation of calcium mobilization, or cell adhesive properties; a slight reduction in the potency of the M15K mutant at stimulating chemotaxis was seen. Our approach identified two key residues, namely Lys-7 and Arg-47, as important amino acids for interaction with CX3CR1. These mutant FKNs displayed reduced affinity for CX3CR1 in steady state whole cell binding assays and did not support either efficient capture or firm adhesion of CX3CR1-expressing cells under physiological flow. However, despite similar effects of these mutations on FKN-CX3CR1-binding interactions, differential effects of these mutations on CX3CR1 signaling were observed. Mutation of Arg-47 produced a protein largely ineffective at stimulating either intracellular calcium mobilization or chemotaxis, at least at the concentrations tested. However, mutation of Lys-7 yielded a molecule that retained most of the agonist activity, although maximal chemotaxis of the L1.2 cells expressing CX3CR1 toward the K7A mutant was slightly reduced. More notable, the potency of this mutant in this assay was similar to the wild type FKN, which was not expected based on measures of affinity in the whole cell binding assays. This difference may reflect receptor host cell differences (L1.2 versus CHO). Alternatively, K7A may be stimulating chemotaxis via a state of CX3CR1 that is not sensitive to mutation at this amino acid position in FKN. Measurement of the relative binding affinities of the mutant panel in the whole cell binding assay is based upon inhibition of the binding of subsaturating levels of 125I-FKN-CD and thus represents binding to a high affinity state of the receptor. This state of the receptor may be all or partially distinct from the population of receptors that mediate the chemotaxis response. Nonetheless, the results identify regions of the FKN-CD that differentiate binding and signaling properties of the FKN molecule.

Slight reductions in steady state binding affinity and potency in the chemotaxis assay were observed for the M15K mutant. Met-15 was previously suggested to be a key determinant in the interaction of the FKN-CD with CX3CR1. From solution NMR measurements of bacterially expressed protein, Mizoue et al. (17) determined that this residue displayed a significant chemical shift upon titration with a chemically synthesized peptide corresponding to the N terminus of human CX3CR1. Our approach toward identifying amino acids in FKN important for CX3CR1 binding and signaling was based on investigation of these interactions using longer forms of the FKN molecule, expressed by mammalian cells as either a membrane-attached or -secreted form, as well as full-length, mammalian cell-expressed receptor. Thus, the identification of Lys-7 and Arg-47 as critical amino acids suggests that regions other than the N terminus of CX3CR1 contribute to the binding energy. Furthermore, post-translational modifications of the receptor may also confer FKN binding determinants.

Shed forms of FKN are evident in both transfected and native cell systems (3, 9). Transfection of 293 cells with the full-length form of FKN leads to the detection of both cell surface protein as well as protein in the conditioned media (3). Brief exposure of primary rat neurons with glutamate leads to increased levels of shed FKN and corresponding decreases in membrane-attached FKN. This neurotoxicity-induced proteolytic cleavage of FKN may be mediated by specific proteases as the matrix metalloproteinase inhibitor batimastat prevents this cleavage (20). Alterations in the levels of low molecular weight forms of FKN are also evident in the facial motor nucleus after peripheral nerve injury and may play a role in neuronal regeneration (9). The presence of various forms of shed FKN could suggest distinct physiological roles for the cleaved forms of the protein. Previously published data (4, 6, 18) indicated that FKN-CD and FKN-CD with the entire mucin stalk were equally effective at binding and stimulating CX3CR1; these earlier studies did not address the functionality of the FKN-CD with intermediate lengths of the mucin stalk. In order to examine possible biphasic effects of the mucin stalk region (i.e. determine if shorter stalks reduced or enhanced a FKN-CX3CR1 interaction), we generated five soluble recombinant FKN constructs in which the FKN-CD contains various lengths of the mucin stalk. Western blot analysis of each of these forms demonstrated that each form migrated in SDS-PAGE at molecular masses greater than the masses predicted by their respective protein backbones. This indicates that glycosylation of the mucin stalk occurs along the entire length of this domain. From a functional standpoint, our data indicate that the length of the stalk does not influence either the binding affinity of the FKN-CD for CX3CR1 or the efficacy in stimulating a calcium mobilization response in receptor-expressing cells. Although there may be multiple sites of cleavage in the FKN mucin domain, the physiological significance of potentially distinct forms of shed FKN remains to be determined. Furthermore, the context within which the receptor is expressed, i.e. the specific cell type, may determine the differential sensitivity toward various shed forms of FKN.

In summary, these data exemplify the importance of amino acid residues within the FKN-CD as the critical determinants in an FKN-CX3CR1 interaction. The structure of the FKN-CD has been reported using both NMR and x-ray crystallography (17, 21). Considering this structural information, the side chains of Lys-7 and Arg-47, while remotely distant from one another, appear to extend out from the same face of the FKN-CD (Fig. 8). Thus, it is conceivable that both of these residues are simultaneously interacting with specific, different amino acid residues found in the extracellular domain of CX3CR1. Mutation of both residues interfered to a similar extent, with the ability of FKN to bind to CX3CR1, as measured in steady state binding and adhesion assays. However, the K7A mutant retained the potency and most of the efficacy of the FKN for stimulating chemotaxis of CX3CR1-expressing cells. The results indicate that residues within the FKN-CD differentially regulate binding to and activation of CX3CR1. Site-directed mutagenesis of other chemokines has revealed similar interactions between these chemokines and their cognate receptors. For instance, mutation of the Arg residue in the ELR motif of interleukin-8 (equivalent to Lys-7 in FKN) yields a molecule that displays reduced affinity for its neutrophil receptor (22). Similarly, mutation of Lys-49 in MCP-1, an amino acid found in the analogous position as Arg-47 of the FKN-CD, yields a mutant with reduced affinity for CCR2 (23). Therefore, common mechanisms of chemokine-chemokine receptor protein interactions seem likely. The identification of the specific counter amino acids residing on the receptor, which interact specifically with the FKN-CD, will require additional experimentation and will have to include further mutational analysis and/or structural determination of the receptor in complex with the ligand. Toward the latter goal, the recent demonstration of the x-ray crystal structure of rhodopsin (24) suggests that more precise structures of other members of the G-protein-coupled receptor superfamily may be forthcoming. Identification of specific FKN-CX3CR1 interactions will aid future development of therapeutic agents that target this receptor system in disease.

    ACKNOWLEDGEMENTS

We thank Aron Boney for performing the FKN ELISA assays and Drs. Art Edison for help with constructing the FKN-CD model, Wolfgang Streit for assistance in culturing rat microglia, and Stephen Baker for critical discussions of the data.

    FOOTNOTES

* This work was supported in part by National Institutes of Health Grants NS34901 (to J. K. H.) and AR39162 (to D. D. P) and an American Heart Association-Florida Affiliate grant-in-aid (to J. K. H.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence may be addressed: Dept. of Pharmacology and Therapeutics, College of Medicine, Box 100267, University of Florida, Gainesville, FL 32610-0267. Tel.: 352-392-3227; Fax: 352-392-9696; E-mail: harrison@pharmacology.ufl.edu.

Dagger Dagger To whom correspondence may be addressed: Depts. of Medicine and Immunology, Duke University Medical Center, Box 2632, 223 MSRB, Durham, NC 27710. Tel.: 919-684-4234; Fax: 919-681-9399; E-mail: patel003@mc.duke.edu.

Published, JBC Papers in Press, March 8, 2001, DOI, 10.1074/jbc.M010261200

    ABBREVIATIONS

The abbreviations used are: FKN, fractalkine; CD, chemokine domain; CX3CR1, receptor for fractalkine; DMEM, Dulbecco's modified Eagle's medium; FBS, fetal bovine serum; PBS, phosphate-buffered saline; CHO, Chinese hamster ovary; HEK, human embryonic kidney; Ni-NTA, nickel-nitrilotriacetic acid; BSA, bovine serum albumin; PAGE, polyacrylamide gel electrophoresis; PCR, polymerase chain reaction; WT, wild type; ELISA, enzyme-linked immunosorbent assay; mAb, monoclonal antibody.

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

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