Alleviation of a Defect in Protein Folding by Increasing the Rate of Subunit Assembly*

Lili A. Aramli and Carolyn M. TeschkeDagger

From the University of Connecticut, Department of Molecular and Cell Biology, Storrs, Connecticut 06269-3125

Received for publication, February 26, 2001


    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Understanding the nature of protein grammar is critical because amino acid substitutions in some proteins cause misfolding and aggregation of the mutant protein resulting in a disease state. Amino acid substitutions in phage P22 coat protein, known as tsf (temperature-sensitive folding) mutations, cause folding defects that result in aggregation at high temperatures. We have isolated global su (suppressor) amino acid substitutions that alleviate the tsf phenotype in coat protein (Aramli, L. A., and Teschke, C. M. (1999) J. Biol. Chem. 274, 22217-22224). Unexpectedly, we found that a global su amino acid substitution in tsf coat proteins made aggregation worse and that the tsf phenotype was suppressed by increasing the rate of subunit assembly, thereby decreasing the concentration of aggregation-prone folding intermediates.


    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The primary amino acid sequence of a polypeptide encodes all of the information necessary for folding and assembly pathways, as well as the native three-dimensional structure (2). Substitutions and deletions in the amino acid sequence of a protein can have a significant impact on the ability of a protein to fold or assemble properly. Depending on the protein, such changes in the amino acid sequence can lead to protein misfolding, mislocalization caused by misfolding, or aggregation (3, 4). Amino acid substitutions in p53 lead to a misfolding problem compromising the function of the protein, resulting in cancer (5). Further, there are the diseases that result from mislocalization caused by protein misfolding. For example, in alpha 1-antitrypsin deficiency, a single amino acid substitution results in the misfolding of alpha 1-antitrypsin, leading to the accumulation of long chain polymers within the hepatocyte. This leads to a reduction of the plasma concentrations of alpha -antitrypsin and predisposes individuals to emphysema and liver disease (6). Therefore, understanding the nature of protein grammar is of paramount interest.

Although the process of protein folding is still not completely understood, it is known that larger proteins often have identifiable folding intermediates. These folding intermediates may interact inappropriately before reaching the native state. In fact, protein misfolding is a common problem faced by biotechnology companies that harvest proteins of commercial interest using recombinant DNA technologies in heterologous hosts (7-11). Often these proteins have problems with inclusion body formation, thereby decreasing the yield of pharmaceutically important products. Single amino acid substitutions can affect the folding pathway by shifting the folding from the productive pathway to off-pathway aggregation. For example, the amino acid substitutions in transthyretin causes a shift in the equilibrium between the native state and an aggregation-prone unfolding intermediate, resulting in amyloid formation. Individuals with any of the 50 known amino acid substitutions in transthyretin are predisposed to familial amyloidosis (12-15). During the folding of P22 tailspike proteins with tsf (temperature-sensitive folding) amino acid substitutions, a folding intermediate is aggregation-prone at high temperatures (16-19). Other proteins such as interluekin-1beta (20) and bovine growth hormone (21) have similar tendencies to aggregate.

We use coat protein of bacteriophage P22 as a model system to study the processes of folding and assembly in vivo and in vitro (22). P22 is a double-stranded DNA bacteriophage of Salmonella typhimurium. The T=7 icosahedral capsid is composed primarily of 420 coat protein subunits, each of which is a 47-kDa polypeptide of 430 amino acids. During the process of assembly, the monomeric coat protein subunits interact with 150-300 molecules of scaffolding protein (33 kDa) in a nucleation-dependent reaction to produce the procapsid, a precursor of the mature capsid. The nucleation-limited assembly reaction occurs by the addition of the monomeric coat protein subunits to the growing edge of the partially formed procapsid (23). Once the procapsid has assembled, the scaffolding protein exits through the holes present in the procapsid lattice while the DNA is actively packaged through the portal vertex. During this process there is an expansion of the capsid lattice into the mature capsid, which is characterized by a 15% increase in diameter, a change in shape from the spherical to icosahedral, and the partial closing of holes in the lattice (24-26).

Previously, a group of amino acid substitutions in phage P22 coat protein that result in a tsf phenotype were identified and characterized (27, 28). In vivo, the tsf amino acid substitutions significantly reduce the yield of soluble coat protein at high temperatures because the newly synthesized tsf coat polypeptides aggregate to form inclusion bodies prior to reaching the mature assembly-competent state required for capsid assembly (27, 29). As a consequence of the tsf defect, there is a decrease in the rate and yield of procapsid assembly both in vitro and in vivo (29, 30). In vitro, tsf coat protein monomers have altered secondary and tertiary structure, as well as increased surface hydrophobicity (30). Additionally, the tsf amino acid substitutions cause an increase in the rate of unfolding of coat protein, thereby increasing the concentrations of the folding intermediates.1 These in vitro properties may explain the increased propensity of the folding intermediates to aggregate.

Based on the above observations, we present a model of folding and assembly of coat protein (Fig. 1). It has been established that the folding of coat protein proceeds through at least two intermediates ([I1] and [I2]) to form an assembly-competent coat protein subunit (30). In tsf coat proteins, at high temperatures, there is an accumulation of an aggregation-prone, off-pathway intermediate ([I*]) from either [I1] or [I2] (30). The reaction producing an aggregate is one of two essentially irreversible reactions in the folding of coat protein. The second irreversible reaction is the assembly of procapsids from the monomeric subunit, which occurs upon the addition of scaffolding protein (31). The tsf coat proteins assemble with slower kinetics than wild type (WT)2 coat protein as a result of the tsf folding defect (30) and assembly does not correct the conformational defects of tsf coat proteins (32).


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Fig. 1.   A model of the folding and assembly of coat protein. In our model, U represents the unfolded coat protein, [I1] and [I2] represent the intermediates, [I*] represents an off-pathway intermediate that is aggregation-prone, and N represents the folded state of coat protein monomers and assembles into procapsids in association with scaffolding protein. The irreversible reactions are indicated with the heavy unidirectional arrows, whereas the reversible reactions are shown with the lighter, bidirectional arrows. The tsf amino acid substitutions increase the rate of unfolding from the coat protein monomeric subunit to [I2].1

As a means of identifying additional amino acids that are critical for folding, second site su (suppressor) amino acid substitutions of tsf coat protein mutants were isolated (1). The most frequently isolated type of second site suppressors were global suppressors. Global suppressor amino acid substitutions are capable of alleviating the phenotype of multiple tsf mutants. These global suppressors were identified at positions 163, 166, and 170 in the amino acid sequence of coat protein (1), a region located in close proximity of a putative hinge domain of coat protein (33).

Here we examine the mechanism by which a global suppressor alleviates the tsf phenotype. Unexpectedly, we found that the presence of the global suppressor amino acid substitution T166I in the tsf coat proteins S223F and F353L lead to an increase in aggregation. Further, we have determined that the increase in aggregation of the tsf:su coat proteins is not likely to be the result of a decrease in the thermostability of the tsf:su coat proteins relative to the tsf parents. However, by a novel mechanism, the tsf phenotype was suppressed by an increase in the rate and yield of subunit assembly.

    EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Bacteria-- The bacteria used for all of the experiments were derivatives of S. typhimurium LT2. The amber suppressor minus host DB7136 (leuA414-am, hisC525-am) and its amber suppressor plus derivative DB7155 (leuA414-am, his C525-am, supE20-gln) have been described previously (34).

Bacteriophage-- The P22 bacteriophage used in this study were WT in gene 5, which encodes for coat protein, carried the tsf nucleotide substitutions in gene 5 leading to amino acid substitutions S223F or F353L, or carried tsf:su mutations in gene 5 leading to S223F/T166I or F353L/T166I (1). All phage strains used in these experiments carried the c1-7 allele, which prevents lysogeny. The phage also carried amber mutations in genes 3 and 13, to prevent DNA packaging and cell lysis, respectively, and to produce procapsids.

Chemicals-- Ultrapure guanidine hydrochloride, urea, and silver nitrate were purchased from Schwartz-Mann ICN. HGT Seakem agarose was purchased from American Bioanalytical. All other chemicals were reagent grade purchased from common sources.

Media-- LB was prepared as described by Life Technologies, Inc. and was used for initial bacterial growth of S. typhimurium, DB7155. A superbroth was prepared to support bacterial growth for procapsid isolation. The superbroth contained 32 g of tryptone, 20 g of yeast extract, 5 g of NaCl, and 5 ml of 1 N NaOH/liter of water (35).

Buffer-- The buffer utilized in all of the experiments was 20 mM sodium phosphate, pH 7.6. For procapsid preparations and storage of the shell stocks, the buffer utilized was 50 mM Tris base, 25 mM NaCl, and 2 mM EDTA, adjusted to pH 7.6 with HCl.

Purification of Coat Proteins-- WT, S223F, S223F/T166I, F353L, and F353L/T166I used in the following experiments were obtained from empty procapsid shell stocks that were prepared as previously described (31, 32, 36, 37). Briefly, S. typhimurium (DB7155) were grown in LB to 1 × 108 cells/ml at 30 °C and infected with various strains of bacteriophage P22 at a multiplicity of infection of 0.075 to prepare a large phage stock. To determine whether reversions of the amber mutations or the tsf phenotype had occurred, the phage stocks were checked for reversion frequency and temperature sensitivity. Next, S. typhimurium (DB7136, an amber minus strain) were grown in the superbroth to 4 × 108 cells/ml at 28 °C with vigorous aeration and infected with bacteriophage P22 at a multiplicity of infection of 5. Because the phage carried amber mutations that prevent cell lysis and DNA packaging, the infected cells accumulated procapsids. After 5 h the chilled cells were pelleted by centrifugation at 4 °C at 10,000 rpm using a GSA rotor in a Sorvall RC-5B Superspeed centrifuge. The collected cells were suspended in a small volume of cold buffer. The cells were lysed by a freeze/thaw cycle and were treated with a final concentration of 0.1 M phenylmethylsulfonyl fluoride and then with 50 µg/ml RNase and 50 µg/ml DNase. The procapsids were pelleted in a Ti60 rotor in a Beckman L7-65 at 45,000 rpm for 35 min at 4 °C. The procapsid pellet was resuspended in a small amount of buffer with fresh phenylmethylsulfonyl fluoride by shaking overnight at 4 °C. The procapsids were purified using a Sephacryl S-1000 (Amersham Pharmacia Biotech) column to remove smaller contaminating proteins and membranes. To prepare empty procapsid shells that are composed solely of coat protein, the scaffolding protein was removed from the procapsids by repeated extractions with 0.5 M guanidine hydrochloride followed by centrifugation to pellet the shells. The shell stock concentrations were determined by absorbance of the unfolded shell stocks in 6 M guanidine hydrochloride using an extinction coefficient of 0.957 ml mg-1 cm-1 at 280 nm (38). All purified empty procapsid shells were suspended in buffer and stored indefinitely at 8.5 mg/ml at 4 °C.

Refolding of Coat Protein by Rapid Dilution-- Empty procapsid shells were unfolded at 2 mg/ml in 6.75 M urea, 20 mM phosphate buffer at pH 7.6 for 30 min at room temperature. Refolding was initiated by rapid dilution with phosphate buffer to yield a final coat protein concentration of 0.1 mg/ml with 0.34 M residual urea.

Refolding of Coat Protein by Dialysis-- Empty procapsid shells were unfolded in urea as described above. The denatured samples were diluted to 1.2 mg/ml and 4.0 M urea and then refolded by dialysis in a microdialyzer (Life Technologies, Inc.) at 4 °C at a rate of 0.75 ml/min with phosphate buffer. The refolded coat protein was collected when urea was no longer detected by refractometry. The samples were treated in a microcentrifuge for 2 min at 13,000 rpm at 4 °C. The protein concentration was determined by absorbance at 280 nm.

Native Polyacrylamide Gel and Agarose Electrophoresis-- The samples for native polyacrylamide gels were prepared by combining a portion of the protein with 3× native gel sample buffer (30% glycerol, 112 mM Tris, and 120 mM glycine). The samples (0.4 µg) were loaded onto native 4.3% polyacrylamide stacking gel (pH 8.3) and 7.5% native polyacrylamide resolving gel (pH 9.5) (39). Native polyacrylamide gels were run at 10 mA constant current at 4 °C for ~1 h. The bands on the native polyacrylamide gels were visualized by silver staining (40). The samples for the native agarose gel were prepared by combining a portion of the protein with agarose gel sample buffer (40 mM Tris base, 1 mM EDTA, 20% sucrose, pH 8.2, with acetic acid), and ~ 6 µg was loaded onto 1.2% Seakem HGT agarose gel made with the same buffer without the sucrose and run at 50 V for 3.5 h at room temperature (41). The agarose gels were stained with Coomassie Blue (10% acetic acid containing 0.03% Coomassie Brilliant Blue R-250 and 0.02% Coomassie Brilliant Blue G-250) overnight and destained (10% acetic acid and 10% isopropyl alcohol) over several days.

Procapsid Assembly Reactions-- To assemble coat protein into procapsids, refolded coat protein at a final concentration of 0.83 mg/ml was mixed with scaffolding protein at a final concentration of 1.6 mg/ml at 20 °C in a total volume of 100 µl in the SLM Aminco-Bowman 2 spectrofluorometer. The reaction was monitored by the increase in 90° light scattering at 500 nm with the bandpasses set to 4 nm. Samples from each reaction were run on 1.2% agarose gels as described above. Another method to monitor the assembly reaction is to refold urea-denatured coat protein and urea-denatured scaffolding protein together until the urea was no longer detectable by refractometry (35). Coat protein and scaffolding protein were each unfolded at 2 mg/ml in 6.75 M urea. The protein concentrations for scaffolding protein were determined at 280 nm using an extinction coefficient of 0.48 ml mg-1 cm-1 (42). Samples were refolded together at 1 mg/ml in a microdialyzer at a flow rate of 0.75 ml/min at 20 °C and 30 °C until residual urea was no longer detected. The samples were prepared and run on both native polyacrylamide and agarose gels as described above.

    RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

As a means of identifying additional amino acids that are critical for folding, second site suppressors of P22 tsf coat protein mutants were isolated (1). The most frequently isolated group of second site suppressors were global suppressors at positions 163, 166, and 170 of the coat protein (1). Global suppressors have been identified in other proteins (43-45). For example, the global suppressors of the tsf mutants in P22 tailspike protein have been shown to alleviate folding defects by decreasing the rate of aggregation through stabilization of a folding intermediate (17, 46-48). Here we examine the mechanism by which a global suppressor alleviates the tsf phenotype in coat protein. Coat proteins carrying the global suppressor substitution T166I in the tsf backgrounds were chosen for this study because the temperature-sensitive phenotype of S223F and F353L was alleviated by all three global suppressors. Additionally, T166I was the most frequently isolated global suppressor amino acid substitution and has proven capable of improving the folding and assembly of tsf coat proteins to WT levels (1).

Aggregation of tsf and tsf:su Coat Proteins during Folding-- Based on the results from the tailspike protein experiments, we believed it likely that the global suppressors of coat protein would also decrease the propensity of the protein to aggregate during folding. To determine the tendency of the tsf:su coat proteins to aggregate during folding, WT, S223F, S223F/T166I, F353L, and F353L/T166I coat proteins were first unfolded in denaturant. Refolding was initiated by rapid dilution at various temperatures. Aliquots were taken after refolding had been initiated and run on a native polyacrylamide gel (Fig. 2). The band of highest mobility corresponds to the folded monomeric form, whereas the aggregates are the ladder of bands of slower mobility (11, 18, 30, 49). None of the coat protein samples aggregated at 15 °C or below. The band of monomeric WT coat protein remained constant at all temperatures tested. As previously reported, a small amount of aggregation of WT coat protein was observed at 33 °C and above but appeared to originate from a band of decreased mobility, suggesting that the aggregation was likely due to incorrectly or slowly folding polypeptides that did not run in the monomer position on the native gel (30). In contrast, the tsf mutant S223F began to substantially aggregate at 20 °C. Surprisingly, S223F/T166I demonstrated a significant increase in the rate of aggregation at 20 °C compared with its tsf parent, as seen by an increase in the intensity and the number of bands of slower mobility present in the tsf:su coat protein (Fig. 2). With the increasing temperature, the intensity of the bands of slower mobility further increased and the intensity of the monomeric bands decreased in S223F/T166I over time when compared with its tsf parent, S223F. The increased tendency to aggregate when the su substitution T166I was present was also observed with F353L/T166I, although both F353L and F353L/T166I began to aggregate at a higher temperature than S223F and S223F/T166I. This is consistent with the in vivo phenotype of F353L (1, 50). F353L began to aggregate at 33 °C, whereas F353L/T166I had increased bands of aggregation at the same temperature. Based on this experiment we conclude that the presence of the su substitution T166I unexpectedly increased the propensity for the tsf coat proteins to aggregate during folding.


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Fig. 2.   Aggregation of WT, tsf, and tsf:su coat proteins during folding. Samples of WT, S223F, S223F/T166I, F353L, and F353L/T166I were unfolded in urea as described under "Experimental Procedures." Refolding was initiated by rapid dilution at various temperatures (4-36 °C). Aliquots were taken at 0.3, 3.5, 7.0, 12.0, and 15.0 min after the refolding reaction was initiated and placed in native gel sample buffer and held on ice. The samples were run on native polyacrylamide gel as described under "Experimental Procedures" (30, 39). The bands were visualized by silver staining (40). The band with the highest mobility corresponds to the folded monomeric form of the various coat protein species (solid circles). The bands of slower mobility are the aggregates (brackets).

Aggregation of tsf and tsf:su Coat Proteins from the Folded State-- One possible explanation for why the tsf:su coat proteins were more aggregation-prone was that these monomers were less thermostable than the tsf coat protein monomers. Previously, the folded tsf coat proteins were shown to be about as stable as WT coat protein by differential scanning calorimetry and denaturation with pressure (36, 51). However, recent equilibrium folding and unfolding experiments with the tsf coat protein mutants have indicated that the proteins were generally less stable to denaturant than WT coat protein.1 Therefore, to determine the thermostability of the tsf:su coat proteins, we first refolded tsf and tsf:su coat proteins to their native conformation at 4 °C and then shifted samples to higher temperatures. The circular dichroism and tryptophan fluorescence spectra of the refolded tsf proteins were the same as previously published (30). The spectra of the tsf:su proteins were similar to their tsf parents and were therefore consistent with folded structure (data not shown). Aliquots were taken after the temperature shift-up and run on native polyacrylamide gels to monitor aggregation of the proteins (Fig. 3). WT coat protein was resistant to aggregation at the various temperatures tested up to 39 °C, where aggregation begins, consistent with our previous work (30). The S223F/T166I coat protein was only slightly more aggregation-prone than the S223F coat protein. Similar results were obtained when comparing the thermostability of F353L and F353L/T166I, although they aggregated slightly less than S223F and S223F/T166I. Thus, it appears unlikely that the increase in aggregation of the tsf:su coat proteins was due to a decrease in the thermostability of the folded monomeric state.


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Fig. 3.   Aggregation propensity of WT, tsf, and tsf:su coat proteins from the native state. WT, S223F, S223F/T166I, F353L, and F353L/T166I coat proteins were unfolded in urea as described under "Experimental Procedures." The samples were refolded by dialysis at 4 °C as described under "Experimental Procedures." Temperature shift-up experiments were performed at 4-39 °C. Aliquots were taken at 0, 3.5, 7.0, 12.0, and 15.0 min after the sample was placed in circulating water bath at a specific temperature and immediately placed in native gel sample buffer and held on ice. The samples were run on a native polyacrylamide gel as described under "Experimental Procedures" and silver-stained. The bands of highest mobility correspond to the folded monomeric form of the various coat protein species (solid circles). The bands of slower mobility are the aggregates (brackets) (30).

Assembly of tsf and tsf:su Coat Proteins-- Since the tsf:su coat proteins have an increased propensity to aggregate during folding, the su amino acid substitution must suppress the tsf phenotype at a step other than the [I1 or I2] left-right-arrow [I*] transition (Fig. 1). Because procapsid assembly is the other irreversible step in the folding pathway of coat protein and could potentially shift the folding equilibrium to the right by decreasing the concentration of N, we examined the effect of the su amino acid substitution on this reaction. The samples of WT, S223F, S223F/T166I, F353L, and F353L/T166I coat proteins were refolded by dialysis at 4 °C to form monomeric coat proteins. The refolded coat proteins were mixed with scaffolding protein at 20 °C. The assembly reaction was monitored by the increase in light scattering at 500 nm (Fig. 4). WT coat protein was assembly-competent and formed procapsids, as seen by the increase in light scattering (31). The tsf coat protein S223F was assembly-incompetent, and F353L had low assembly activity. In contrast, addition of the su amino acid substitution in the tsf background dramatically increased both the rate and the yield of procapsid assembly. Because aggregates are large and can also scatter light, we confirmed that the increase in light scattering was the result of procapsid formation by running the assembly reactions on native agarose gels where coat protein was found to be in either procapsid form or as folded monomers (data not shown). Therefore, whereas the su amino acid substitutions increased the aggregation propensity of the tsf:su coat proteins during folding, once folded, procapsid assembly was enhanced.


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Fig. 4.   Assembly of WT, tsf, and tsf:su coat proteins. WT, S223F, S223F/T166I, F353L, and F353L/T166I coat proteins were unfolded in urea as described under "Experimental Procedures." The samples were refolded by dialysis overnight at 4 °C at a flow rate of 0.3 ml/min. Refolded coat protein and refolded scaffolding protein were mixed together and monitored by light scattering at 20 °C as described under "Experimental Procedures" (30).

In vivo, a P22 bacteriophage-infected cell would have scaffolding protein present during the folding of coat protein. To determine whether the presence of scaffolding protein during folding would decrease the aggregation reaction by favoring subunit assembly, unfolded coat and unfolded scaffolding protein were mixed together at equal concentrations and dialyzed at various temperatures until urea was no longer detected (52). The samples were run on a native agarose gel to detect procapsids (Fig. 5A). As expected, when the WT coat protein was folded with WT scaffolding protein, subunit assembly occurred, yielding procapsids. Conversely, the tsf coat proteins S223F and F353L did not assemble into procapsids. The tsf:su coat proteins S223F/T166I and F353L/T166I formed procapsids at both 20 °C (Fig. 5A) and 30 °C (data not shown), although there was a decrease in the yield of the procapsids at the higher temperature. Additionally, the same samples were run on native polyacrylamide gels to monitor aggregation (Fig. 5B). We observed a significant decrease in the intensity of the bands corresponding to aggregates in the reaction where the tsf:su coat proteins were refolded with scaffolding protein as compared with tsf:su coat protein refolded without scaffolding protein (Fig. 5B). Thus, our experiments indicate that the presence of scaffolding protein during folding of the tsf:su coat proteins decreased their tendency for aggregation by increasing the yield and rate of procapsid assembly.


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Fig. 5.   Assembly by refolding of coat protein with scaffolding protein. WT, S223F, S223F/T166I, F353L, and F353L/T166I coat proteins and scaffolding protein were unfolded in urea as described under "Experimental Procedures." After dilution, unfolded coat and unfolded scaffolding protein were refolded together by dialysis at various temperatures (20 and 33 °C) as described under "Experimental Procedures." A, the samples were run on an agarose gel as described under "Experimental Procedures." The bands were visualized by Coomassie staining. B, the samples were run on a native polyacrylamide gel as described under "Experimental Procedures" and silver-stained. The bands of highest mobility correspond to the folded monomeric form of the various coat protein species. The band of slowest mobility, not entering the gel, corresponds to the procapsid. The bands of slower mobility are the aggregates (30).


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Aggregation is a serious problem both for biotechnology utilizing recombinant DNA technologies in heterologous hosts (7-11) and for various proteins with amino acid substitutions such as transthyretin (12-15). Thus, learning to control aggregation is of the utmost importance. Here we have investigated the effect of a global suppressor amino acid substitution on the aggregation propensity of tsf coat proteins in an effort to determine the mechanism of suppression of the tsf folding defects. Surprisingly, we have found that the tendency to aggregate increases in the presence of the suppressor amino acid substitution and that the suppression of the original tsf defect occurs by increasing the rate and yield of subunit assembly. This may have general implications in improving the folding and yield of multimeric proteins.

Global Suppressors-- In addition to our global suppressors of tsf mutants in P22 coat protein (1), other second site suppressors have been found that alleviate an original folding defect. P22 tailspike protein is a well studied protein. A group of tsf mutants of P22 tailspike protein have been identified and characterized to be defective in folding and not stability (16, 53, 54). Analysis of the folding pathway revealed that aggregation of tailspike protein occurs by the association of a partially folded monomeric intermediate rather than the native trimeric species. The thermolabile intermediate preferentially partitions onto the aggregation pathway as the temperature increases (55). To determine other positions in the amino acid sequence important to the folding process, second site suppressors of the tsf tailspike protein mutants were isolated (56). Two major global suppressor substitutions were found to alleviate the folding defects of multiple tsf mutants of tailspike protein (46). Structural resolution of a truncated version of tailspike protein revealed that the two global suppressors, V331A and A334V, were located in the parallel beta -helix domain and the associated "dorsal fin domain" of the fishlike structure. The global suppressors of the tsf mutants in tailspike protein decrease aggregation in vivo and in vitro (47). However, the global suppressor amino acid substitutions act by different mechanisms (48). The suppressor, V331A, alleviates the tsf folding defects by stabilizing the completely folded protein by reducing steric hindrance in the native state. The suppressor, A334V, alleviates the folding defects in a more complicated way. The A334V suppressor substitution accelerates unfolding at high temperature, thereby decreasing the stability of the trimeric tailspike protein. The A334V substitution also increases the stability of an early folding intermediate by improving hydrophobic stacking during folding, therefore improving the overall folding of the tailspike protein.

Examples of global suppressors of folding mutants of proteins other than phage proteins exist. For instance, variants of chloramphenicol acetyltransferase, a bacterial enzyme that confers resistance to the antibiotic chloramphenicol, have been isolated. These mutants quantitatively aggregate into cytoplasmic inclusion bodies, resulting in a lack of chloramphenicol resistance. Van der Schueren et al. (57) isolated second site suppressors of these variants of chloramphenicol acetyltransferase. The global suppressor, L145F, improved the thermostability of the protein and its ability to fold into a soluble, enzymatically active conformation. Similarly, temperature-sensitive mutants of the human receptor-like protein-tyrosine phosphatase LAR have both stability and folding defects that result in the aggregation of the protein. The presence of a suppressor amino acid substitution in leukocyte-antigen related (LAR) decreased the aggregation propensity as seen by an increase in the production of the properly folded protein. The decrease in aggregation occurs, at least in part, by an increase in the stabilization of mutant protein (43). A second amino acid substitution, M182T, is often found along with substitutions in TEM-1 beta -lactamase that confer increased resistance to antibiotics (44). The M182T substitution was shown to decrease the stability of the protein to denaturant but increased the solubility of the double mutant (58). The exact mechanism by which M182T functions to decrease aggregation is still to be established. Here the suppressor substitution has been proposed to either inhibit aggregation by changing the conformation of an aggregation-prone intermediate or to alter the folding mechanism in a way to kinetically avoid aggregation (58).

Mechanisms for Suppression of Folding Defects-- Together, the different suppressor amino acid substitutions of the various mutants of P22 tailspike protein, chloramphenicol acetyltransferase, and human receptor-like protein tyrosine phosphatase LAR have been shown to correct the folding problem by stabilizing either the native conformation of the protein or a folding intermediate. These examples are in contrast to the novel mechanism we have elucidated for tsf:su coat protein mutants. Based on our experiments, it is clear that there is actually an increased propensity of the tsf:su coat protein mutants to aggregate during folding. However, the tsf:su mutant coat proteins have improved subunit assembly capability, thereby suppressing the original tsf phenotype. We believe that this method could be used generally to improve the folding yield of multimeric proteins.

    ACKNOWLEDGEMENTS

We thank Carole Capen, Shannon Doyle, Dr. Todd Garabedian, Walter Nakonechny, and Mark Tardie for helpful comments and discussions.

    FOOTNOTES

* This work was supported by National Institutes of Health Grant GM53567.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Molecular and Cell Biology, U-3125, University of Connecticut, 75 N. Eagleville Rd., Storrs, CT 06269-3125. Tel.: 860-486-4282; Fax: 860-486-4331; E-mail: teschke@uconn.edu.

Published, JBC Papers in Press, April 13, 2001, DOI 10.1074/jbc.M101759200

1 C. M. Teschke, unpublished data.

    ABBREVIATIONS

The abbreviations used are: WT, wild type; LAR, leukocyte-antigen related.

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

1. Aramli, L. A., and Teschke, C. M. (1999) J. Biol. Chem. 274, 22217-22224[Abstract/Free Full Text]
2. Anfinsen, C. B. (1973) Science 181, 223-230[Medline] [Order article via Infotrieve]
3. Thomas, P. J., Qu, B.-H., and Pedersen, P. L. (1995) Trends Biochem. Sci. 20, 456-459[CrossRef][Medline] [Order article via Infotrieve]
4. Welch, W. J., and Brown, C. R. (1996) Cell Stress Chaperones 1, 109-115[Medline] [Order article via Infotrieve]
5. Vogelstein, B., and Kinzler, K. W. (1992) Cell 70, 523-526[Medline] [Order article via Infotrieve]
6. James, E. L., Whisstock, J. C., Gore, M. G., and Bottomley, S. P. (1999) J. Biol. Chem. 274, 9482-9488[Abstract/Free Full Text]
7. Marston, F. A. O. (1986) Biochem. J. 240, 1-12[Medline] [Order article via Infotrieve]
8. Mitraki, A., and King, J. (1989) BioTechnology 7, 690-697
9. Teschke, C. M., and King, J. (1992) Curr. Opin. Biotechnol. 3, 468-473[Medline] [Order article via Infotrieve]
10. Thatcher, D. R., and Hitchcock, A. (1992) in Mechanisms of Protein Folding (Pain, R. H., ed) , pp. 229-261, Oxford University Press, New York
11. Betts, S. D., and King, J. (1999) Struct. Fold. Des. 7, R131-R139[Medline] [Order article via Infotrieve]
12. Lai, Z., Colon, W., and Kelly, J. W. (1996) Biochemistry 35, 6470-6482[CrossRef][Medline] [Order article via Infotrieve]
13. Kelly, J. W. (1997) Structure 5, 595-600[Medline] [Order article via Infotrieve]
14. Kelly, J. W., Colon, W., Lai, Z., Lashuel, H. A., McCulloch, J., McCutchen, S. L., Miroy, G. J., and Peterson, S. A. (1997) in Protein Misassembly (Wetzel, R., ed), Vol. 50 , pp. 161-181, Academic Press, San Diego
15. Kelly, J. W. (1998) Curr. Opin. Struct. Biol. 8, 101-106[CrossRef][Medline] [Order article via Infotrieve]
16. Goldenberg, D. P., and King, J. (1981) J. Mol. Biol. 145, 633-651[Medline] [Order article via Infotrieve]
17. Mitraki, A., Danner, M., King, J., and Seckler, R. (1993) J. Biol. Chem. 268, 20071-20075[Abstract/Free Full Text]
18. Speed, M. A., Wang, D. I. C., and King, J. (1996) Nat. Biotechnol. 14, 1283-1287[Medline] [Order article via Infotrieve]
19. Betts, S. D., and King, J. (1998) Protein Sci. 7, 1516-1523[Abstract/Free Full Text]
20. Chrunyk, B. A., Evans, J., Lillquist, J., Young, P., and Wetzel, R. (1993) J. Biol. Chem. 268, 18053-18061[Abstract/Free Full Text]
21. Brems, D. N. (1988) Biochemistry 27, 4541-4546
22. Prevelige, P. E., Jr., and King, J. (1993) Prog. Med. Virol. 40, 206-221[Medline] [Order article via Infotrieve]
23. Prevelige, P. E., Jr., Thomas, D., and King, J. (1993) Biophys. J. 64, 824-835[Abstract]
24. King, J., and Casjens, S. (1974) Nature 251, 112-119[Medline] [Order article via Infotrieve]
25. Earnshaw, W., Hendrix, R., and King, J. (1980) J. Mol. Biol. 134, 575-594
26. Prasad, B. V. V., Prevelige, P. E., Jr., Marieta, E., Chen, R. O., Thomas, D., King, J., and Chiu, W. (1993) J. Mol. Biol. 231, 65-74[CrossRef][Medline] [Order article via Infotrieve]
27. Gordon, C. L., and King, J. (1993) J. Biol. Chem. 268, 9358-9368[Abstract/Free Full Text]
28. Gordon, C. L., and King, J. (1994) Genetics 136, 427-438[Abstract/Free Full Text]
29. Nakonechny, W. S., and Teschke, C. M. (1998) J. Biol. Chem. 273, 27236-27244[Abstract/Free Full Text]
30. Teschke, C. M. (1999) Biochemistry 38, 2873-2881[CrossRef][Medline] [Order article via Infotrieve]
31. Prevelige, P. E., Jr., Thomas, D., and King, J. (1988) J. Mol. Biol. 202, 743-757[Medline] [Order article via Infotrieve]
32. Capen, C. M., and Teschke, C. M. (2000) Biochemistry 39, 1142-1151[CrossRef][Medline] [Order article via Infotrieve]
33. Lanman, J., Tuma, R., and Prevelige, P. E., Jr. (1999) Biochemistry 38, 14614-14623[CrossRef][Medline] [Order article via Infotrieve]
34. Winston, R., Botstein, D., and Miller, J. H. (1979) J. Bacteriol. 137, 433-439[Medline] [Order article via Infotrieve]
35. Fuller, M. T., and King, J. (1981) Virology 112, 529-547[Medline] [Order article via Infotrieve]
36. Galisteo, M. L., Gordon, C. L., and King, J. (1995) J. Biol. Chem. 270, 16595-16601[Abstract/Free Full Text]
37. Teschke, C. M., and Fong, D. G. (1996) Biochemistry 35, 14831-14840[CrossRef][Medline] [Order article via Infotrieve]
38. Teschke, C. M., and King, J. (1993) Biochemistry 32, 10839-10847[Medline] [Order article via Infotrieve]
39. Andrews, A. T. (1986) in Electrophoresis: Theory, Techniques and Biochemical and Clinical Application (Peacocke, A. R. , and Harrington, W. F., eds) , pp. 5-78, Oxford University Press, New York
40. Rabilloud, T., Carpentier, G., and Tarroux, P. (1988) Electrophoresis 9, 288-291[Medline] [Order article via Infotrieve]
41. Serwer, P., and Pichler, M. E. (1978) J. Virol. 28, 917-928[Medline] [Order article via Infotrieve]
42. Greene, B., and King, J. (1999) J. Biol. Chem. 274, 16135-16140[Abstract/Free Full Text]
43. Tsai, A. Y. M., Toh, M., Streuli, M., Thai, T., and Saito, H. (1991) J. Biol. Chem. 266, 10534-10543[Abstract/Free Full Text]
44. Huang, W., and Palzkill, T. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 8801-8806[Abstract/Free Full Text]
45. McGee, W. A., and Nall, B. T. (1998) Protein Sci. 7, 1071-1082[Abstract/Free Full Text]
46. Fane, B., Villafane, R., Mitraki, A., and King, J. (1991) J. Biol. Chem. 266, 11640-11648[Abstract/Free Full Text]
47. Mitraki, A., Fane, B., Haase-Pettingell, C., Sturtevant, J., and King, J. (1991) Science 253, 54-58[Medline] [Order article via Infotrieve]
48. Schuler, B., and Seckler, R. (1998) J. Mol. Biol. 281, 227-234[CrossRef][Medline] [Order article via Infotrieve]
49. Speed, M. A., Wang, D. I. C., and King, J. (1995) Protein Sci. 4, 900-908[Abstract/Free Full Text]
50. Gordon, C. L., Sather, S. K., Casjens, S., and King, J. (1994) J. Biol. Chem. 269, 27941-27951[Abstract/Free Full Text]
51. Foguel, D., Teschke, C. M., Prevelige, P. E., Jr., and Silva, J. L. (1995) Biochemistry 34, 1120-1126[Medline] [Order article via Infotrieve]
52. Fuller, M. T., and King, J. (1980) Biophys. J. 32, 381-401[Abstract]
53. Goldenberg, D. P., Smith, D. H., and King, J. (1983) Proc. Natl. Acad. Sci. U. S. A. 80, 7060-7064[Abstract]
54. Goldenberg, D. P. (1988) Annu. Rev. Biophys. Biophys. Chem. 17, 481-507[Medline] [Order article via Infotrieve]
55. Haase-Pettingell, C., and King, J. (1988) J. Biol. Chem. 263, 4977-4983[Abstract/Free Full Text]
56. Fane, B., and King, J. (1991) Genetics 127, 263-277[Abstract/Free Full Text]
57. Van der Schueren, J., Robben, J., and Volckaert, G. (1998) Protein Eng. 11, 1211-1217[Abstract]
58. Sideraki, V., Huang, W., Palzkill, T., and Gilbert, H. F. (2001) Proc. Natl. Acad. Sci. U. S. A. 98, 283-288[Abstract/Free Full Text]


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