Hypusine Is Required for a Sequence-specific Interaction of
Eukaryotic Initiation Factor 5A with Postsystematic Evolution of
Ligands by Exponential Enrichment RNA*
Aiguo
Xu
and
Kuang Yu
Chen
§¶
From the
Department of Chemistry, Rutgers-The State
University of New Jersey and § The Cancer Institute of New
Jersey, Piscataway, New Jersey 08854-8087
Received for publication, October 2, 2000
 |
ABSTRACT |
Hypusine is formed through a
spermidine-dependent posttranslational modification of
eukaryotic initiation factor 5A (eIF-5A) at a specific lysine residue.
The reaction is catalyzed by deoxyhypusine synthase and deoxyhypusine
hydroxylase. eIF-5A is the only protein in eukaryotes and
archaebacteria known to contain hypusine. Although both eIF-5A and
deoxyhypusine synthase are essential genes for cell survival and
proliferation, the precise biological function of eIF-5A is unclear. We
have previously proposed that eIF-5A may function as a bimodular
protein, capable of interacting with protein and nucleic acid (Liu,
Y. P., Nemeroff, M., Yan, Y. P., and Chen, K. Y. (1997)
Biol. Signals 6, 166-174). Here we used the method of
systematic evolution of ligands by exponential enrichment (SELEX) to
identify the sequence specificity of the potential eIF-5A RNA targets.
The post-SELEX RNA obtained after 16 rounds of selection exhibited a
significant increase in binding affinity for eIF-5A with an apparent
dissociation constant of 1 × 10
7
M. The hypusine residue was found to be critical for this
sequence-specific binding. The post-SELEX RNAs shared a high sequence
homology characterized by two conserved motifs, UAACCA and AAUGUCACAC.
The consensus sequence was determined as AAAUGUCACAC by sequence
alignment and binding studies. BLAST analysis indicated that this
sequence was present in >400 human expressed sequence tag sequences.
The C terminus of eIF-5A contains a cold shock domain-like structure, similar to that present in cold shock protein A (CspA). However, unlike
CspA, the binding of eIF-5A to either the post-SELEX RNA or the
5'-untranslated region of CspA mRNA did not affect the sensitivity of these RNAs to ribonucleases. These data suggest that the
physiological significance of eIF-5A-RNA interaction depends on
hypusine and the core motif of the target RNA.
 |
INTRODUCTION |
Eukaryotic initiation factor 5A
(eIF-5A),1 ubiquitously present
in eukaryotes and archaebacteria, but not in eubacteria, is the only
protein known to contain a hypusine residue (for review, see Refs.
1-3). Hypusine is formed in two steps: (i) deoxyhypusine synthase
catalyzes the transfer of a 4-aminobutyl moiety from spermidine to a
specific lysine residue to form a deoxyhypusine residue,
N
-(4-aminobutyl)lysine; and (ii)
deoxyhypusine hydroxylase catalyzes the hydroxylation of the
deoxyhypusine residue to form hypusine (N
-(4-amino-2-hydroxybutyl)lysine). The fact
that nature has committed two enzymes to produce one hypusine residue
on a single protein underscores the importance of this
posttranslational modification. Hypusine formation is tightly coupled
to cell proliferation and is essential for cell survival (1-3).
Disruption of either the eIF-5A or deoxyhypusine synthase gene in yeast
leads to a lethal phenotype (4-6). Inhibition of deoxyhypusine
synthase in mammalian cells causes growth arrest (7-9), cell death
(10), or tumor differentiation (9). In addition, hypusine formation
activity exhibits a marked increase in virally transformed cells (11) but a striking attenuation in senescent cells (12).
Despite the importance of eIF-5A in cell proliferation and survival,
the physiological function of this protein is unclear. The notion that
eIF-5A is an initiation factor comes from the earlier observations that
it can be isolated from the ribosome-bound fraction and that it can
stimulate the synthesis of methionyl-puromycin (13-15). However, the
role of eIF-5A in translation initiation has been questioned because of
a lack of correlation between eIF-5A and general protein synthesis
(16-18). Recent studies have suggested that eIF-5A may serve as a
target protein for the human immunodeficiency virus type I Rev protein
(19) and the human T-cell leukemia virus type 1 Rex protein (20).
However, conflicting data have appeared, and direct evidence of
interaction between eIF-5A and viral proteins is lacking (21).
X-ray diffraction studies of the eIF-5A precursor from two archaea
species show that it is composed of two domains connected by a flexible
hinge (22, 23). The N-terminal domain contains the hypusine residue,
which carries two positive charges and closely resembles spermidine and
spermine. The C-terminal domain consists of five
-strands, which
closely resemble the cold shock domain (CSD) present in bacterial cold
shock protein A (CspA). These studies suggest that eIF-5A may interact
with nucleic acids, particularly RNA. This notion is substantiated by
our previous finding that eIF-5A is capable of binding to Rev response
element (RRE) and U6 RNA in vitro (25) and the finding that
the TIF51A gene, which encodes eIF-5A in yeast, could
complement the temperature-sensitive growth and mRNA decay
phenotypes of ts1159 mutant yeast (24).
In the present study we have used the strategy of systematic evolution
of ligands by exponential enrichment (SELEX; Ref. 26) to enrich RNA
sequences that bind to eIF-5A with high affinity. We demonstrated that
the RNAs enriched over 16 rounds of SELEX shared a high sequence
homology, and, more importantly, the binding of eIF-5A to the selected
RNAs requires the presence of the hypusine residue.
 |
EXPERIMENTAL PROCEDURES |
Materials--
[
-32P]UTP and
[
-32P]ATP (3,000 Ci/mmol) were obtained from ICN
Chemical Radioisotope Division (Irvine, CA). The yeast expression vector pYES2 was a gift of Drs. Herbert and Celia Tabor (National Institutes of Health). Glutathione S-transferase-Rev and
pGEM-RRE were given by Dr. Michael H. Malim (University of
Pennsylvania), and pET11-CspA and pJJGO2 containing the CspA
gene were given by Dr. Masayori Inoyue (University of Medicine and
Dentistry of New Jersey). All NTPs and RNase inhibitor were purchased
from Roche Molecular Biochemicals. Restriction enzymes and other
molecular biological supplies including SP6 RNA polymerase and T7 RNA
polymerase were from Promega or Amersham Pharmacia Biotech.
Expression of Unmodified and Modified Histidine-tagged
eIF-5A--
The plasmid pQEh18K containg histidine-tagged the human
eIF-5A cDNA insert was constructed from pQTy21 as described
previously (27). The histidine-tagged eIF-5A precursor is termed
6xHis-18K-lys, where 18K and lys refer to the apparent molecular weight
and the unmodified lysine residue at position 50, respectively. The
plasmid pYER18K was constructed by inserting the 6xHis-human eIF-5A
cDNA between the HindIII and EcoRI sites of
yeast expression vector pYES2. The histidine-tagged eIF-5A is termed
6xHis-18K-hyp, where the lysine 50 residue has been converted to the
hypusine residue (hyp). The 6xHis-18K-lys protein was overexpressed in
Escherichia coli strain BL 21 (DE3), and the 6xHis-18K-hyp
protein was overexpressed in yeast (strain 2602). Both 6xHis-18K-lys
and 6xHis-18K-hyp were purified by metal affinity chromatography and
fast protein liquid chromatography.
SELEX--
The oligonucleotide template used for SELEX was first
constructed as a single-stranded 98 mer with the following sequence: 5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC(N)40GGGTCAATGCGTCATA-3', where the central 40 base pairs contained a random sequence (N) based on equal incorporation of A, G, C, and T at each position. The
complementary strand was synthesized by annealing a primer with the
sequence 5'-GCGGGATCCTATGACGCATTGACCC-3' (primer 2) followed by the DNA
polymerase reaction using a Klenow fragment. Primer 1, containing a T7
RNA polymerase promoter sequence, was used for polymerase chain
reaction (PCR) amplification:
5'-GCGGAATCCTAATACGACTCACTATAGGGAACAGTCCGAGCC-3'. Restriction sites for BamHI and EcoRI were
included in both primers for cloning. A degenerate double-stranded DNA
template was synthesized by PCR using primer 1 (T7 primer) and primer 2 (reverse primer). The template obtained from the PCR reaction was used
for in vitro transcription to generate a random pool of RNA
molecules as described (28). The transcribed RNA was purified by
electrophoresis on an 8% polyacrylamide gel. RNA was dissolved in an
annealing buffer (10 mM Tris-HCl, pH 8.0, 10 mM
KCl, and 2 mM MgCl2) heated at 95 °C for 2 min and then put on ice for 20 min before the start of the binding
reaction. To initiate the in vitro selection, the random
pool RNAs were first eluted through a 200-µl Ni-nitrilo triacetic
acid-resin column (Promega). The eluted RNAs were incubated at
4 °C with 6xHis-18K-hyp (2 µM) in a 20 mM
Tris-HCl buffer, pH 8.0, containing 10% glycerol, 1 mM
dithiothreitol, 30 mM KCl, 4 mM
MgCl2, and 3 µg tRNA for 30 min. The binding mixture was then passed through the Ni-nitrilo triacetic acid-resin column (bed
volume 20 µl) and washed with 6 ml of binding buffer containing 0.02 M imidazole and another 4 ml of binding buffer containing 0.05 M imidazole. The bound RNAs were then eluted with the
binding buffer containing 0.4 M imidazole. The eluted RNAs
were reverse-transcribed by avian myeloblastosis virus reverse
transcriptase (20 units; Roche Molecular Biochemicals) using primer 2 in the reverse transcription buffer (Roche Molecular Biochemicals). The
cDNAs obtained were then amplified by PCR using primers 1 and 2. The PCR product was purified by electrophoresis through 8%
polyacrylamide gels, eluted, and transcribed in vitro using
T7 RNA polymerase to generate the pool 1 RNAs for the second round of
selection. After the 16th selection cycle, the cDNAs obtained from
the selected RNAs were cloned into pCR II vector (Invitrogen) for
sequence determination.
RNA Preparation and Labeling--
The 252-nucleotide (nt) RRE
RNA was synthesized by T7 RNA polymerase using linearized pGEM-RRE as
the template in the presence of [
-32P]UTP and purified
by electrophoresis as described (29). The RNAs with sizes of 23, 44, and 86 nt were synthesized by transcribing of the fragment obtained
from restriction enzyme digestion of pBluescript IISK with,
respectively, XhoI, HindIII, and XbaI. The sequences of these RNAs are: 23 nt, GGCGAAUUGGGUACCGGGCCCCC; 44 nt,
GGCGAAUUGGGUACCGGGCCCCCCCUCGAGGUCGACGGUAUCG; and 86 nt, GGCGAAUUGGGUACCGGGCCCCCCCUCGAGGUCGACGGUAUCGAUAAGCUUGAUAUCGAAUUCCUGCAGCCCGGGGGAUCCACUA. The random RNAs (pre-SELEX RNA) were synthesized using the 98 mer
containing N40 as the template. The post-SELEX RNAs were obtained by T7
polymerase transcription of the selected oligonucleotides as template.
All the RNA probes were radiolabeled with [
-32P]UTP by
T7 RNA ploymerase, and the labeled RNA probes were gel-purified by
electrophoresis using a 12% polyacrylamide gel. The labeled RNA
was eluted with a solution containing 0.3 M sodium acetate, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA, and 0.5%
SDS at 37 °C. For preparation of single-stranded DNA (ssDNA)
probes, the specific primer was first labeled with
[
-32P] ATP by T4 kinase and PCR amplification, the
labeled PCR products were heated to 100 °C, and ssDNA was separated
by electrophoresis on an 8% polyacrylamide gel.
Filter Binding Assay--
The filter binding assay was carried
out in a final volume of 15 µl binding buffer (10 mM
Tris-HCl, pH 8.0, 2 mM MgCl2 10 mM
KCl, 10% glycerol, 2 mM dithiothreitol, 0.25 mg/ml bovine
serum albumin (BSA), 0.5 mg/ml yeast tRNA) containing 6xHis-18K-hyp and
fixed amounts of RNA (~50 pM). The mixture was incubated
at 4 °C for 20 min. The binding mixture was then filtered through a
0.45-µm BA85 nitrocellulose filter paper (Schleicher & Schuell). The
filter paper was thoroughly washed with a solution containing 10 mM Tris-HCl, pH 8.0, 2 mM MgCl2,
and 10 mM KCl. Radioactivity retained on the filter paper
was measured by an LS650 liquid scintillation counter. The retention of
free RNA (always <10%) was substracted from all data points.
Gel Mobility Shift Assay--
Radioactively labeled RNA or ssDNA
(~10 fmol) was incubated with 6xHis-18K-hyp or 6xHis-18K-lys protein
for 15 min on ice in 15 µl of binding buffer (10 mM
Tris-HCl, pH 8.0, 2 mM MgCl2, 10 mM
KCl, 10% glycerol, 2 mM dithiothreitol, 0.25 mg/ml BSA, yeast tRNA at indicated concentrations). The stringent conditions referred to the binding reactions carried out in the presence of high
tRNA concentrations (>2 µM). The binding mixture was
loaded onto an 8% polyacrylamide gel made in Tris borate-EDTA buffer containing 5% glycerol, and electrophoresis was carried out at 4 °C. After the electrophoresis the gel was vacuum-dried and
visualized by autoradiography.
Ribonuclease Protection Assay--
RNA probes were radiolabeled
with [
-P32]UTP during in vitro
transcription. The labeled RNA was incubated with various amounts of
purified 6xHis-18K-hyp or other recombinant proteins in the RNA binding
buffer for 15 min on ice. RNase T1 or RNase A was then added to the
binding mixture to initiate RNA digestion. The reaction mixture was
kept on ice for 15 min and analyzed by electrophoresis on a 12%
polyacrylamide gel. The gel was fixed and dried on a DE-81 paper for autoradiography.
 |
RESULTS |
Binding of Histidine-tagged eIF-5A to RRE RNA--
Fig.
1A shows the purification of the
recombinant eIF-5A precursor (6xHis-18K-lys) from E. coli
and the recombinant eIF-5A (6xHis-18K-hyp) from yeast (Fig. 1A,
lanes 4 and 8). The RNA binding activity of these two
recombinant proteins was compared with that of the wild type HeLa
eIF-5A. Fig. 1B shows that HeLa eIF-5A and recombinant
eIF-5A 6xHis-18K-hyp, but not the eIF-5A precursor 6xHis-18K-lys, could
bind to RRE RNA (Fig. 1B, lanes 5 and 7 versus lane 3), indicating that the histidine tag
did not interfere with the RNA binding activity of recombinant eIF-5A.
We therefore could use 6xHis-18K-hyp affinity chromatography to
initiate the in vitro selection.

View larger version (42K):
[in this window]
[in a new window]
|
Fig. 1.
A, purification of unmodified and
modified eIF-5A. Lanes 1 and 5, protein markers,
lane 2, flowthrough; lane 3, eluent from a
Ni-nitrilo triacetic acid column; lane 4, purified
6xHis-18K-lys from fast protein liquid chromatography; lane
6, uninduced yeast extracts; lane 7, induced yeast
extracts; lane 8, 6xHis-18K-hyp purified from a Ni-nitrilo
triacetic acid column and fast protein liquid chromatography (Mono-S).
B, binding of RRE RNA with 6xHis-18K-lys, HeLa eIF-5A, and
6xHis-18K-hyp. Lane 1, RRE RNA alone; lanes 2 and
3, RRE RNA plus 6xHis-18K-lys (0.5 and 2.0 µg);
lanes 4 and 5, RNA probe with HeLa eIF-5A (0.5 and 2.0 µg); lanes 6 and 7, RNA probe with
modified eIF-5A, 6xHis-18K-hyp (0.5 and 2.0 µg).
|
|
Size Requirement of RNA for eIF-5A Binding--
To determine the
optimal size of RNA required for in vitro selection, we have
tested the binding of eIF-5A to RNAs of different sizes under
nonstringent conditions. Fig. 2 shows that
6xHis-18K-hyp did not bind to the 23- or 44-nt RNA (Fig. 2, lanes
1-6) but could bind to the longer RNA with a length of 86 nt
(Fig. 2, lanes 8 and 9 versus lane 7).
On the basis of this result, we designed a DNA template with a total
length of 98 nt, consisting of a variable region of 40 nt (N40) and a
5'-end-flanking region containing the T7 RNA polymerase promoter. Both
5'- and 3'-end-flanking regions were designed to avoid
self-complementarity and to minimize secondary structure formation that
may bias the selection (30).

View larger version (30K):
[in this window]
[in a new window]
|
Fig. 2.
Size dependence of the binding of eIF-5A with
RNA. RNA fragments of different lengths were generated by
transcribing the pBlueScriptII SK fragments in vitro using
T7 RNA polymerase. The XhoI fragment gives 23 nt; the
HindIII fragment gives 44 nt; and the XbaI
fragment gives 86 nt. Lane 1, RNA (23 nt); lane
2, RNA (23 nt) with eIF-5A (2 µg); lane 3, RNA (23 nt) with eIF-5A (8 µg); lane 4, RNA (44 nt); lane
5, RNA (44 nt) with eIF-5A (2 µg); lane 6, RNA (44 nt) with eIF-5A (8 µg); lane 7, RNA (86 nt); lane
8, RNA (86 nt) with eIF-5A (2 µg); lane 9, RNA (86 nt) with eIF-5A (8 µg).
|
|
In Vitro Selection of RNA Specifically Recognized by Human
eIF-5A--
The 98-nt template was used to generate a DNA pool
consisting of ~1024 unique sequences. The DNA pool was
transcribed in vitro to give a random RNA pool, termed
pre-SELEX RNA. The selection was initiated with the random RNA pool,
using 6xHis-18K-hyp as the bait protein (round 0). The RNA pool
enriched after 16 cycles of selection was termed post-SELEX RNA.
Post-SELEX RNA was then tested for binding with 6xHis-18K-hyp using a
gel mobility shift assay. Fig. 3A
shows that the binding affinity of 6xHis-18K-hyp for the post-SELEX RNA
was ~10-fold greater than that for random RNA under nonstringent conditions (Fig. 3A, lane 4 versus lane
2). However, the difference in binding affinity became quite
pronounced under a more stringent binding condition. Thus, with a
higher concentration of tRNA (>2 µM) in the binding
buffer, the binding of eIF-5A to random RNA was completely abolished
(Fig. 3B, lanes 3 and 4 versus
lane 2). In contrast, the binding between eIF-5A and
post-SELEX RNA remained intact even in the presence of 20 µM tRNA (Fig. 3B, lane 8). We also compared
the binding of eIF-5A to RRE and U6 RNA with that to post-SELEX RNA.
Fig. 4 shows that eIF-5A bound to all three RNAs equally well under nonstringent conditions (Fig. 4, lanes 2, 6, and 10). However, under more stringent conditions,
eIF-5A bound only to post-SELEX RNA, but not RRE or U6 RNA (Fig. 4,
lanes 8 and 12 versus lane
4). Although the binding of eIF-5A to RRE or U6 RNA appeared
to be less specific, we cannot rule out the possibility that such
binding could still serve some useful functions in vivo.

View larger version (35K):
[in this window]
[in a new window]
|
Fig. 3.
Binding of 6xHis-18K-hyp with random and
post-SELEX RNA. The 32P-labeled RNA probe (10,000 cpm)
was incubated with eIF-5A, and the binding complex was resolved on a
native polyacrylamide gel (8%; acrylamide/bis-acrylamide = 29)
by electrophoresis. Positions of free RNA and protein-RNA complex are
shown by arrows. A, lane 1, random RNA alone;
lane 2, random RNA with 6xHis-18K-hyp (2 µg); lane
3, post-SELEX RNA; lane 4, post-SELEX RNA with
6xHis-18K-hyp (2 µg). B, lane 1, random RNA
alone; lanes 2-4, random RNA with 6xHis-18K-hyp (2 µg) in
the presence of E. coli tRNA (0.2, 2, and 20 µM, respectively); lane 5, post-SELEX RNA
alone; lanes 6-8, post-SELEX RNA with 6xHis-18K-hyp (2 µg) in the presence of E. coli tRNA (0.2, 2, and 20 µM, respectively).
|
|

View larger version (30K):
[in this window]
[in a new window]
|
Fig. 4.
Binding of 6xHis-18K-hyp with post-SELEX RNA,
U6 RNA, and RRE RNA. The 32P-labeled RNA probe (10,000 cpm) was incubated with 6xHis-18K-hyp at a final concentration of 4 µM, and the binding complex was analyzed by
electrophoresis on a native polyacrylamide gel (8%;
acrylamide/bis-acrylamide = 29). Lane 1, Post-SELEX
RNA alone; lanes 2-4, Post-SELEX RNA probe with
6xHis-18K-hyp in the presence of increasing amounts of E. coli tRNA (0.2, 2, and 20 µM, respectively);
lane 5, U6 RNA alone; lanes 6-8, U6 RNA with
6xHis-18K-hyp in the presence of increasing amounts of E. coli tRNA (0.2, 2, and 20 µM, respectively);
lane 9, RRE RNA alone; lanes 10-12, RRE RNA with
6xHis-18K-hyp in the presence of increasing amounts of E. coli tRNA (0.2, 2, and 20 µM, respectively).
|
|
Role of Hypusine Residue in the Binding of eIF-5A to Post-SELEX
RNA--
To determine the role of hypusine in RNA binding, we compared
the RNA binding activity of eIF-5A (6xHis-18K-hyp) with that of its
precursor (6xHis-18K-lys). Fig. 5 shows that
both BSA, an unrelated protein, and 6xHis-18K-lys did not exhibit any
binding activity with either random or the post-SELEX RNA (Fig. 5,
lanes 2-6 and 11-15). In contrast,
6xHis-18K-hyp exhibited strong binding with the post-SELEX RNA but not
with random RNA (Fig. 5, lanes 16-18 versus
lanes 7-9). Because the difference between 6xHis-18K-hyp and 6xHis-18K-lys is limited to only one amino acid residue, namely, hypusine versus lysine, the striking difference in their
binding activities toward post-SELEX RNA argues strongly for the
important role of hypusine in the eIF-5A-RNA interaction.

View larger version (34K):
[in this window]
[in a new window]
|
Fig. 5.
The hypusine residue is required for
eIF-5A-RNA binding. Radiolabeled and gel-purified random RNA or
post-SELEX RNA was incubated with BSA, 6xHis-18K-lys, or 6xHis-18K-hyp
at different concentrations. The reaction mixtures were then analyzed
by gel mobility shift assay on an 8% native gel. Lane 1,
random RNA probe alone; lanes 2 and 3, random RNA
with BSA (5 and 10 µM); lanes 4-6, random RNA
with 6xHis-18K-lys (7, 15, and 30 µM, respectively);
lanes 7-9, random RNA with 6xHis-18K-hyp (7, 15, and 30 µM, respectively); lane 10, post-SELEX RNA
probe alone; lanes 11 and 12, post-SELEX RNA with
BSA (5 and 10 µM); lanes 13-15, post-SELEX
RNA probe wih 6xHis-18K-lys (7, 15, and 30 µM,
respectively); lanes 16-18, post-SELEX RNA probe with
6xHis-18K-hyp (7, 15, and 30 µM, respectively).
18Klys, 6xHis-18K-lys; 18Khyp,
6xHis-18K-hyp.
|
|
Sequences and Binding Affinity of Cloned Post-SELEX RNA--
To
characterize the nature of post-SELEX RNA, we have isolated independent
clones from both pre- and post-SELEX RNA pools for sequence
determination and binding study. Table I
lists the sequences of these RNA clones. The individual post-SELEX RNA
clones exhibited strong binding to eIF-5A under stringent conditions (Fig. 6A, lanes 3, 6, 9, and
12). Assuming a 1:1 binding stoichiometry, the apparent
Kd value for the eIF-5A-RNA binding complex was
estimated to be ~1 × 10
7 (Fig.
6B). The apparent Kd value for the
binding of eIF-5A to random RNA was difficult to estimate because of
the low binding affinity under the stringent conditions. The maximal level of binding of eIF-5A with post-SELEX RNA was >100-fold higher than that with pre-SELEX RNA.

View larger version (25K):
[in this window]
[in a new window]
|
Fig. 6.
Binding characteristics of 6xHis-18K-hyp with
cloned post-SELEX RNA. A, gel mobility shift assay of
the binding between 6xHis-18K-hyp and independently cloned post-SELEX
RNA. Lane 1, clone 1 RNA alone; lanes 2 and
3, clone 1 RNA with 6xHis-18K-hyp (0.7 and 3.5 µM); lane 4, clone 2 RNA alone; lanes
5 and 6, clone 2 RNA with 6xHis-18K-hyp (0.7 and 3.5 µM); lane 7, clone 3 RNA alone; lanes
8 and 9, clone 3 RNA with 6xHis-18K-hyp (0.7 and 3.5 µM); lane 10, clone 4 RNA alone; lanes
11 and 12, clone 4 RNA with 6xHis-18K-hyp (0.7 and 0.5 µM); lane 13, random RNA probe; lanes
14 and 15 random RNA with 6xHis-18K-hyp (0.7 and 3.5 µM). B, filter paper binding assay. The filter
paper binding assay was carried out using variable amounts of
6xHis-18K-hyp with a fixed amount of 32P-labeled random RNA
probe (×) or post-SELEX RNA clones ( , , and ).
|
|
Consensus Sequences and BLAST Analysis--
With the sequences of
post-SELEX RNA available (Table I), we proceeded to examine whether
they shared any sequence homology. Fig. 7
shows that, on the basis of sequence alignment, the consensus sequence
of post-SELEX RNA can be defined as either CCUAACCACGCGCCU (sequence I)
or CUAAAUGUCACAC (sequence II). If gap formation is allowed, the
consensus sequence can also be defined as sequence I + II
(CCUAACCACGCGCCUnnCUAAAUGUCACAC). To determine which one is important
for eIF-5A binding, we have generated four additional 98-nt RNA
sequences (Table II, probes 2-5) containing
either sequence I or sequence II and compared their binding affinity to
eIF-5A with that of post-SELEX RNA. Table II shows that RNAs containing sequence II (probes 4 and 5) retained high binding affinity with eIF-5A, whereas RNAs containing sequence I (probes 2 and 3) exhibited reduced binding affinity with eIF-5A, indicating that sequence II is
required for high affinity eIF-5A binding. Because the two RNAs
containing sequence II at different positions exhibited similar binding
affinity to eIF-5A (Table II, probe 4 versus probe 5), the
position of sequence II within the 98-nt RNA sequence may not be
crucial for eIF-5A binding. We also generated a 40-nt RNA with the
sequence identical to that of the variable region (N40) of the clone 6 post-SELEX RNA. We found that the binding affinity of eIF-5A with the
40-nt RNA was only ~
of that obtained with the 98-nt
post-SELEX RNA (data not shown), suggesting that the flanking regions
are likely to contribute to the overall stabilization of the eIF-5A-RNA
complex.

View larger version (61K):
[in this window]
[in a new window]
|
Fig. 7.
Sequence alignment of post-SELEX RNA.
The conserved region was identified by sequence alignment and is
indicated by shading. In case (iii) sequence
alignment was performed by the Genetics Computer Group Pileup program
with gap formation allowed for maximal alignment. The consensus
sequence in each case was determined on the basis of the frequency of
appearance of a specific nucleotide. The nucleotides that appeared with
a frequency 64% are indicated. Nucleotides that appear with a
frequency >90% are underlined. n, no specific
nucleotide appears at that position with a frequency >50%.
|
|
Next we searched for a possible occurrence of sequences I and II in the
GenBank or EST data base by BLAST analysis. We found that the 11-nt
sequence I (AACCACGCGCCU) is present in 53 EST sequences, and the 11-nt
sequence II (AAAUGUCACAC) is present in 474 sequences. However, no
match could be found for the 30-nt sequence I + II in either the
GenBank or EST data base. Although the sequence II motif appears to be
important in the sequence-specific binding of eIF-5A, it remains to be
investigated whether the EST sequences that contain sequence II could
serve as the physiological targets of eIF-5A in vivo. Along
this line, we are currently using the specific nucleic acids associated
with proteins method (31) to identify the physiological ligands of
eIF-5A.
Binding of 6xHis-18K-hyp to ssDNA--
Most CSD-containing
proteins are capable of binding to ssDNA (32). We therefore examined
whether eIF-5A could also interact with ssDNA. Both sense and antisense
single-stranded DNA were generated from random and post-SELEX RNA
clones for gel mobility shift assay. Fig. 8
shows that eIF-5A could bind to the sense ssDNA derived from post-SELEX
RNA (Fig. 8, lane 7), similar to CspA (Fig. 8, lanes
2 and 3 versus lane 1). However,
when eIF-5A was used at a lower concentration, the mobility of ssDNA
was only partially retarded and appeared as a diffused band (Fig. 8,
lane 6, band a). We suspected that the band was attributable
to the interaction of CSD with ssDNA, because: (i) a similar diffused band was apparent when the eIF-5A precursor was used in the binding assay (Fig. 8, lanes 4 and 5, band a);
and (ii) the C-terminal half of eIF-5A (eIF-5A 84-154) could partially
retard the ssDNA (Fig. 8, lane 8). The antisense ssDNA from
either random or post-SELEX RNA or the sense ssDNA derived from
pre-SELEX RNA did not bind to eIF-5A under the same binding conditions
(data not shown). These data suggest that the eIF-5A-ssDNA binding was
sequence-specific.

View larger version (27K):
[in this window]
[in a new window]
|
Fig. 8.
The binding of eIF-5A with ssDNA. ssDNA
was prepared as described under "Experimental Procedures."
Lane 1, ssDNA only; lanes 2 and 3,
ssDNA with CspA (0.5 and 3.0 µg); lanes 4 and
5, ssDNA with 6xHis-18K-lys (0.5 and 3 µg); lanes
6 and 7, ssDNA with 6xHis-18K-hyp (0.5 and 3 µg);
lane 8, ssDNA with CSD (eIF-5A 84-154; 3 µg). The binding
mixture was analyzed by electrophoresis on an 8% polyacrylamide gel.
Bands a and b, retarded protein-DNA
complexes.
|
|
Functional Assay--
Because eIF-5A shares a certain structural
similarity with CspA (22, 23), it is tempting to speculate that they
may share some functional similarity. CspA has been proposed to
function as an RNA chaperon because it facilitates the
ribonuclease-catalyzed degradation of 5'-untranslated region (UTR) CspA
RNA (32). It is therefore of interest to test whether eIF-5A shows a
similar effect in the same assay system. A direct comparison of the
effect of eIF-5A with that of CspA on the 5'-UTR degradation seems
warranted, because both proteins appeared to bind to the 5'-UTR equally
well under the experimental conditions (Fig.
9, lanes 2 and 5 versus lane 1). Whereas the binding of CspA to
the 5'-UTR could facilitate the degradation of the RNA (Fig. 9,
lane 4 versus lane 3), as previously
reported (32), the binding of eIF-5A to the 5'-UTR did not alter the
sensitivity of the RNA to RNase T1 (Fig. 9, lanes 9-11
versus lanes 6-8). We then performed a similar
ribonuclease assay using the post-SELEX RNA as the target RNA. Fig.
10 shows that the ribonuclease digestion
patterns of post-SELEX RNA with either RNase A or RNase T1 were almost
identical whether measured in the absence or in the presence of eIF-5A
(Fig. 10, lanes 7-10 versus lanes
3-6 and lanes 15-18 versus lanes
11-14). These results suggest that eIF-5A may not functionally
resemble CspA, at least under the present assay system.

View larger version (70K):
[in this window]
[in a new window]
|
Fig. 9.
Effects of CspA and eIF-5A on the RNase
T1-catalyzed degradation of 5'-UTR CspA mRNA. Ribonuclease
assay was performed as described under "Experimental Procedures."
The RNA substrate was derived from the 5'-UTR of the CspA mRNA with
the region from +1 to +142 placed under a T7 promoter. The RNA was
labeled with with [ -32P]UTP. The RNase assay was
carried out at 15 °C for 10 min. The amount of RNase T1 is shown in
units. The protein CspA or 6xHis-18K-hyp at 3 µg/assay was added (+)
with the RNA before RNase digestion. The final reaction volume was 15 µl. Lane 1, RNA substrate alone; lane 2, RNA
with CspA; lane 3, RNase T1 (1 unit) was added to RNA;
lane 4, RNase T1 (1 unit) was added to RNA with CspA;
lane 5, RNA with 6xHis-18K-hyp; lanes 6-8, 10, 1, and 0.1 units of RNase T1, respectively, added to RNA; lanes
9-11, 10, 1, and 0.1 units of RNase T1, respectively, added to
RNA with 6xHis-18K-hyp. Immediately after the reaction, the reaction
mixture was loaded onto an 8% acrylamide gel, and electrophoresis was
carried out at 150 V at 4 °C.
|
|

View larger version (83K):
[in this window]
[in a new window]
|
Fig. 10.
Effect of eIF-5A binding on the degradation
of RNA catalyzed by RNase T1 or RNase A. Ribonuclease assay was
performed as described under "Experimental Procedures." The cloned
post-SELEX RNA (clone 3) was radiolabeled with
[ -32P]UTP and used as the substrate. The reaction
mixture (final volume of 15 µl) was incubated for 10 min at 15 °C.
The amount of ribonuclease is shown in µg. The eIF-5A protein,
6xHis-18K-hyp, was added at the amount of 3 µg/assay before the
addition of RNase T1 or RNase A. Lane 1, clone 3 RNA alone;
lane 2, RNA plus 6xHis-18K-hyp; lanes 3-6,
0.063, 0.125, 0.25, and 0.5 µg of RNase T1, respectively, added to
the reaction mixture; lanes 7-10, same as lanes
3-6, except that 6xHis-18K-hyp (3 µg) was first mixed with RNA;
lanes 11-14, 0.05, 0.1, 0.2, and 0.4 µg of RNase A,
respectively, added to the reaction mixture; lanes 15-18,
same as lanes 11-14, respectively, except that
6xHis-18K-hyp (3 µg/assay) was first mixed with RNA. Immediately
after the reaction, the reaction mixture was applied to a 12%
polyacrylamide gel, and electrophoresis was carried out at 150 V at
4 °C.
|
|
 |
DISCUSSION |
eIF-5A is the only cellular protein known to contain hypusine
(1-3). Although eIF-5A has been shown to be essential for growth and
proliferation, its precise physiological function is still unclear. On
the basis of the finding that eIF-5A can bind to RRE and U6 RNA, we
have proposed that eIF-5A may function as an RNA-binding protein (25).
In the present study we have used SELEX, an in vitro
selection method, to enrich and identify RNAs that may specifically bind to eIF-5A. The enriched RNAs, termed post-SELEX RNAs, shared a
high sequence homology (Table I and Fig. 7) and showed enhanced binding
affinity with eIF-5A (Fig. 6). The binding specificity between eIF-5A
and post-SELEX RNA was quite high, as indicated by the observation that
the binding occurred even in the presence of 20 µM tRNA
(Figs. 3 and 4). The finding that eIF-5A can bind to RNA in a
sequence-specific manner suggests that eIF-5A may indeed function as an
RNA-binding protein. More importantly, the binding of eIF-5A to the
selected RNA requires the presence of a hypusine residue (Fig. 5),
suggesting that hypusine has a role in the eIF-5A-RNA interaction and
that the hypusine-dependent RNA binding might be
biologically relevant. The notion that eIF-5A may selectively affect
the expression and/or degradation of a small group of certain mRNAs
(18, 24) is consistent with possibility that some of the EST sequences
that contain sequence II may serve as the physiological ligands of
eIF-5A.
X-ray structures of archaebacterial eIF-5A reveal the presence of
hypusine at the N-terminal domain and CSD in the C-terminal domain (22,
23). Although both the hypusine site and CSD have the potential to
interact with RNA, the finding that eIF-5A, but not the eIF-5A
precursor, binds to post-SELEX RNA suggests that the hypusine site may
be more critical than CSD in the sequence-specific eIF-5A-RNA
interaction. In this regard, it may not be surprising that eIF-5A
behaves differently from CspA, the namesake of CSD, in the ribonuclease
protection assay (Figs. 8 and 9). However, we cannot rule out the
possibility that eIF-5A may still function as an RNA chaperon with
certain other target RNAs.
RNA-binding proteins such as Rev and NS1 are bimodular in that they
have a nucleic acid binding domain containing a basic amino acid
cluster at the N terminus and a leucine-rich domain at the C terminus
used for protein interaction (33). Motif analysis shows the presence a
stretch of basic amino acids clustered at the hypusine site and a
leucine-rich stretch at the C-terminal domain in eIF-5A (25). Thus,
instead of binding only with RNA, eIF-5A may use its bimodular
structure to interact with both RNA and protein. Indeed, eIF-5A has
been shown to interact with deoxyhypusine synthase (35),
transglutaminase II (36), L5 (37), CRM1 (38), and exportin 4 (39). It remains to be determined whether any of these proteins is
truly the physological substrate of eIF-5A. Nevertheless, these studies
and the finding that eIF-5A can bind to RNA in a sequence-specific
manner support the notion that eIF-5A is a bimodular RNA-binding
protein. RNA-binding proteins have diverse functions related to RNA
processing, turnover, nucleocytoplasmic transport, and transcription
and translation (33, 34). Although the precise functions of eIF-5A
in vivo have been difficult to define, the possibility that
eIF-5A may function as an RNA-binding protein provides useful clues on
the physiological functions of eIF-5A in living cells.
 |
ACKNOWLEDGEMENTS |
We are grateful to Dr. Michael H. Malim
(University of Pennsylvania) for pGEM-RRE and glutathione
S-transferase-Rev, Drs. Herbert Tabor and Celia Tabor for
pYES2, and Dr. Masayori Inouye for the CspA plasmid. We also thank Dr.
Rashmi Gupta in our group for constructing pYER18K and pYER21K.
 |
FOOTNOTES |
*
This work was supported in part by United States Public
Health Services Grant RO1 CA49695 from the NCI, National Institutes of
Health.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
¶
To whom correspondence should be addressed: Dept. of
Chemistry, Rutgers-The state University of New Jersey, P. O. Box
939, Piscataway, NJ 08855-0939. Tel.: 732-445-3739; Fax: 732-445-5312; E-mail: KYCHEN@rutchem.rutgers.edu.
Published, JBC Papers in Press, November 1, 2000, DOI 10.1074/jbc.M008982200
 |
ABBREVIATIONS |
The abbreviations used are:
eIF-5A, eukaryotic
initiation factor 5A;
SELEX, systematic evoluton of ligands by
exponential enrichment;
6xHis-18K-lys, histidine-tagged human eIF-5A
precursor;
6xHis-18K-hyp, histidine-tagged human eIF-5A;
RRE, Rev
response element;
CSD, cold shock domain;
CspA, cold shock protein A;
hyp, hypusine;
PCR, polymerase chain reaction;
nt, nucleotide;
ss, single-stranded;
BSA, bovine serum albumin;
EST, expressed sequence
tag;
UTR, untranslated region.
 |
REFERENCES |
1.
|
Park, M. H.,
Wolff, E. C.,
and Folk, J. E.
(1993)
Biofactors
4,
95-104[Medline]
[Order article via Infotrieve]
|
2.
|
Chen, K. Y.,
and Liu, A. Y.
(1997)
Biol. Signals
6,
105-109[Medline]
[Order article via Infotrieve]
|
3.
|
Park, M. H.,
Lee, Y. B.,
and Joe, Y. A.
(1997)
Biol. Signals
6,
115-123[Medline]
[Order article via Infotrieve]
|
4.
|
Schnier, J.,
Schwelberger, H. G.,
Smit-McBride, Z.,
Kang, H. A.,
and Hershey, J. W. B.
(1991)
Mol. Cell. Biol.
11,
3105-3114[Medline]
[Order article via Infotrieve]
|
5.
|
Sasaki, K.,
Abid, M. R.,
and Miyazaki, M.
(1996)
FEBS Lett.
384,
151-154[CrossRef][Medline]
[Order article via Infotrieve]
|
6.
|
Park, M. H.,
Joe, Y. A.,
and Kang, K. R.
(1998)
J. Biol. Chem.
273,
1677-1683[Abstract/Free Full Text]
|
7.
|
Park, M. H.,
Wolff, E. C.,
Lee, Y. B.,
and Folk, J. E.
(1994)
J. Biol. Chem.
269,
27827-27832[Abstract/Free Full Text]
|
8.
|
Shi, X. P.,
Yin, K. C.,
Ahern, J.,
Davis, L. J.,
Stern, A. M.,
and Waxman, L.
(1996)
Biochim. Biophys. Acta
1310,
119-126[Medline]
[Order article via Infotrieve]
|
9.
|
Chen, Z. P.,
Yan, Y. P.,
Ding, Q. J.,
Knapp, S.,
Potenza, J. A.,
Schugar, H. J.,
and Chen, K. Y.
(1996)
Cancer Lett.
105,
233-239[CrossRef][Medline]
[Order article via Infotrieve]
|
10.
|
Tome, M. E.,
and Gerner, E. W.
(1997)
Biol. Signals
6,
150-156[Medline]
[Order article via Infotrieve]
|
11.
|
Chen, Z. P.,
and Chen, K. Y.
(1997)
Cancer Lett.
115,
235-241[CrossRef][Medline]
[Order article via Infotrieve]
|
12.
|
Chen, Z. P.,
and Chen, K. Y.
(1997)
J. Cell. Physiol.
170,
248-254[CrossRef][Medline]
[Order article via Infotrieve]
|
13.
|
Benne, R.,
Brown-Luede, M.,
and Hershey, J. W. B.
(1978)
J. Biol. Chem.
253,
3070-3075[Medline]
[Order article via Infotrieve]
|
14.
|
Park, M. H.
(1989)
J. Biol. Chem.
264,
18531-18535[Abstract/Free Full Text]
|
15.
|
Smit-McBride, Z.,
Schnier, J.,
Kaufma, R. J.,
and Hershey, J. W. B.
(1989)
J. Biol. Chem.
264,
18527-18530[Abstract/Free Full Text]
|
16.
|
Duncan, R. F.,
and Hershey, J. W.
(1986)
J. Biol. Chem.
261,
12903-12906[Abstract/Free Full Text]
|
17.
|
Gordon, E. D.,
Mora, R.,
Meredith, S. C.,
and Lindquist, S. L.
(1987)
J. Biol. Chem.
262,
16590-16595[Abstract/Free Full Text]
|
18.
|
Kang, H. A.,
and Hershey, J. W. B.
(1994)
J. Biol. Chem.
269,
3934-3940[Abstract/Free Full Text]
|
19.
|
Ruhl, M.,
Himmelspach, M.,
Bahr, G. M.,
Himmerschmid, F.,
Jaksche, H.,
Wolff, B.,
Aschauer, H.,
Farrington, G. K.,
Probst, H.,
and Bevec, D.
(1993)
J. Cell Biol.
123,
1309-1320[Abstract]
|
20.
|
Katahira, J.,
Ishizaki, T.,
Sakai, H.,
Adachi, A.,
Yamamoto, K.,
and Shida, H. J.
(1995)
J. Virol.
69,
3125-3133[Abstract]
|
21.
|
Mattaj, I. W.,
and Englmeier, L.
(1998)
Annu. Rev. Biochem.
67,
265-306[CrossRef][Medline]
[Order article via Infotrieve]
|
22.
|
Peat, T. S.,
Newman, J.,
Waldo, G. S.,
Berendzen, J.,
and Terwilliger, T. C. S.
(1998)
Structure
6,
1207-1214[Medline]
[Order article via Infotrieve]
|
23.
|
Kim, K. K.,
Hung, L. W.,
Yokota, H.,
Kim, R.,
and Kim, S. H.
(1998)
Proc. Natl. Acad. Sci. U. S. A.
95,
10419-10424[Abstract/Free Full Text]
|
24.
|
Zu, D.,
and Jacobson, A.
(1998)
EMBO J.
17,
2914-2925[Abstract/Free Full Text]
|
25.
|
Liu, Y. P.,
Nemeroff, M.,
Yan, Y. P.,
and Chen, K. Y.
(1997)
Biol. Signals
6,
166-174[Medline]
[Order article via Infotrieve]
|
26.
|
Turek, C.,
and Gold, L.
(1990)
Science
1249,
505-510
|
27.
|
Tao, Y.,
and Chen, K. Y.
(1994)
Biochem. J.
302,
517-525[Medline]
[Order article via Infotrieve]
|
28.
|
Milligan, J. F.,
and Uhlenbeck, O. C.
(1989)
Methods Enzymol.
180,
51-62[Medline]
[Order article via Infotrieve]
|
29.
|
Heaphy, S.,
Dingwall, C.,
Ernberg, I.,
Gait, M.,
Green, S. M.,
Karn, J.,
Lowe, A. D.,
Singh, M.,
and Skinner, M. A.
(1990)
Cell
60,
685-693[Medline]
[Order article via Infotrieve]
|
30.
|
Berglund, J. A.
(1997)
Nucleic Acids Res.
25,
1042-1049[Abstract/Free Full Text]
|
31.
|
Triffillis, P.,
Day, N.,
and Kiledjian, M.
(1999)
RNA
5,
1071-1082[Abstract/Free Full Text]
|
32.
|
Jiang, W. N.,
Hou, Y.,
and Inouye, M.
(1997)
J. Biol. Chem.
272,
196-202[Abstract/Free Full Text]
|
33.
|
Burd, C. G.,
and Dreyfuss, G.
(1994)
Science
265,
615-621[Medline]
[Order article via Infotrieve]
|
34.
|
Cusack, S.
(1999)
Curr. Opin. Struct. Biol.
9,
66-73[CrossRef][Medline]
[Order article via Infotrieve]
|
35.
|
Lee, Y. B.,
Joe, Y. A.,
Wolff, E. C.,
Dimitriadis, E. K.,
and Park, M. H.
(1999)
Biochem. J.
340,
273-281[CrossRef][Medline]
[Order article via Infotrieve]
|
36.
|
Singh, U. S.,
Li, Q.,
and Cerione, R.
(1998)
J. Biol. Chem.
273,
1946-1950[Abstract/Free Full Text]
|
37.
|
Schatz, O.,
Oft, M.,
Dascher, C.,
Schebesta, M.,
Rosorius, O.,
Jaksche, H.,
Dobrovnik, M.,
Bevec, D.,
and Hauber, J.
(1998)
Proc. Natl. Acad. Sci. U. S. A.
95,
1607-1612[Abstract/Free Full Text]
|
38.
|
Elfgang, C.,
Rosorius, O.,
Hofer, L.,
Jaksche, H.,
Hauber, J.,
and Bevec, D.
(1999)
Proc. Natl. Acad. Sci. U. S. A.
96,
6229-6234[Abstract/Free Full Text]
|
39.
|
Lipowsky, G.,
Bischoff, F. R.,
Schwarzmaier, P.,
Kraft, R.,
Kostka, S.,
Hartmann, E.,
Kutay, U.,
and Gorlich, D.
(2000)
EMBO J.
19,
4362-4371[Abstract/Free Full Text]
|
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.