The Transport of Group 2 Capsular Polysaccharides across the Periplasmic Space in Escherichia coli

ROLES FOR THE KpsE AND KpsD PROTEINS*

Carlos ArrecubietaDagger§, Tansy C. HammartonDagger, Brendan Barrett, Sorujsiri Chareonsudjai||, Nigel Hodson, David Rainey, and Ian S. Roberts**

From the University of Manchester, 1.800 Stopford Building, School of Biological Sciences, Oxford Road, Manchester, M13 9PT, United Kingdom

Received for publication, September 7, 2000, and in revised form, November 14, 2000



    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The cell surface expression of group 2 capsular polysaccharides involves the translocation of the polysaccharide from its site of synthesis on the inner face of the cytoplasmic membrane onto the cell surface. The transport process is independent of the repeat structure of the polysaccharide, and translocation across the periplasm requires the cytoplasmic membrane-anchored protein KpsE and the periplasmic protein KpsD. In this paper we establish the topology of the KpsE protein and demonstrate that the C terminus interacts with the periplasmic face of the cytoplasmic membrane. By chemical cross-linking we show that KpsE is likely to exist as a dimer and that dimerization is independent of the other Kps proteins or the synthesis of capsular polysaccharide. No interaction between KpsD and KpsE could be demonstrated by chemical cross-linking, although in the presence of both KpsE and Lpp, KpsD could be cross-linked to a 7-kDa protein of unknown identity. In addition, we demonstrate that KpsD is present not only within the periplasm but is also in both the cytoplasmic and outer membrane fractions and that the correct membrane association of KpsD was dependent on KpsE, Lpp, and the secreted polysaccharide molecule. Both KpsD and KpsE showed increased proteinase K sensitivity in the different mutant backgrounds, reflecting conformational changes in the KpsD and KpsE proteins as a result of the disruption of the transport process. Collectively the data suggest that the trans-periplasmic export involves KpsD acting as the link between the cytoplasmic membrane transporter and the outer membrane with KpsE acting to facilitate this transport process.



    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The export of capsular polysaccharides in Gram-negative bacteria from their site of synthesis on the inner face of the cytoplasmic membrane onto the bacterial surface presents a unique challenge to the micro-organism. It requires the translocation of a high molecular weight negatively charged macromolecule across two lipid bilayers. Understanding this process offers potential benefits in terms of engineering polysaccharides of biomedical importance in bacteria and in designing new antimicrobials that inhibit this process. In contrast to protein secretion, our understanding of how capsular polysaccharide transport is achieved is currently scant. The expression of capsular polysaccharides (or K antigens) in Escherichia coli offers an experimentally tractable system in which to try to understand the mechanisms of polysaccharide transport. E. coli can express over 80 chemically and serologically distinct capsular polysaccharides that have been divided into four groups according to a number of biochemical and genetic criteria (1). Group 2 capsules resemble those found on the surfaces of Neisseria meningitidis and Hemophilus influenzae and are often expressed by pathogenic E. coli isolates causing extraintestinal disease (2). The biochemistry and genetics of E. coli group 2 capsules, as typified by the K1 and K5 antigens, have been studied in most detail (reviewed in Refs. 1, 3, and 4). Group 2 capsules are synthesized by a hetero-oligomeric membrane-bound biosynthetic complex on the inner face of the cytoplasmic membrane by the sequential action of glycosyltransferases that elongate the polysaccharide at its nonreducing end (5). A common export pathway, irrespective of the repeat structure of the particular polysaccharide molecule, is then used to translocate group 2 capsules from their site of synthesis onto the cell surface (3, 6). Translocation across the cytoplasmic membrane is mediated by the KpsC, M, S, and T proteins, whereas translocation across the periplasm and outer membrane involves the KpsD and E proteins (1, 3, 4). The KpsC and S proteins are believed to attach phosphatidyl-Kdo1 to the reducing end of the nascent polysaccharide chain, and this substitution permits the entry of the polysaccharide molecule into the export pathway (1, 3, 7). However, the enzymology of this process still awaits elucidation. Translocation of the polysaccharide across the cytoplasmic membrane is achieved by the KpsM and T proteins that constitute an ABC-2 (ATP-binding cassette type 2) transporter (8), in which KpsM is the integral membrane protein and KpsT is the ATPase (9-12). Typically such ABC-2 transporters involved in polysaccharide export require two accessory proteins to complete the export process, a cytoplasmic membrane-periplasmic auxiliary protein (MPA), of which KpsE is an example, and an outer membrane auxiliary protein (8). Based on computer analysis, a predicted topology has been suggested for MPA proteins in which the protein is anchored to the cytoplasmic membrane via N- and C-terminal membrane-spanning domains separated by a large periplasmic domain (8). Typically, outer membrane auxiliary proteins are modified by the signal peptidase II to yield a lipoprotein (8) and, in the case of group I capsular polysaccharides, have been shown to form aggregates in the outer membrane (13). The role of KpsD as an outer membrane auxiliary protein is less clear as it has only limited homology to members of this family (8). In addition, KpsD has a typical N-terminal signal sequence and is believed to be a soluble periplasmic protein (14). As such its role in the export of group 2 capsular polysaccharides is unclear.

In this paper we demonstrate that the C terminus of KpsE interacts with the periplasmic face of the cytoplasmic membrane probably via an amphipathic alpha -helix. By chemical cross-linking we demonstrate that KpsE is likely to exist as a dimer and that dimerization is independent of the other Kps proteins or the synthesis of capsular polysaccharide. No interaction between KpsD and KpsE could be demonstrated by chemical cross-linking although in the presence of both KpsE and Lpp, KpsD could be cross-linked to a 7-kDa protein of unknown identity. In contrast, both KpsD and KpsE showed increased sensitivity to proteinase K when either KpsE or KpsD were absent. The KpsD protein was detected not only in the periplasm but also in both the cytoplasmic and outer membrane fractions, and the correct membrane association of KpsD was dependent on KpsE, Lpp, and the secreted polysaccharide molecule. Collectively, the data suggest that the trans-periplasmic export of group 2 capsular polysaccharide involves KpsD acting as the link between the cytoplasmic membrane transporter and the outer membrane with KpsE acting to facilitate this transport process.


    EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Bacterial Strains and Plasmids-- E. coli strains used in this study are shown in Table I. Bacteria were grown in LB broth supplemented with 100 µg ml-1 ampicillin, 200 µg ml-1 streptomycin, 25 µg ml-1 chloramphenicol, or 75 µg ml-1 kanamycin as required. Plasmid pPC6 confers resistance to chloramphenicol and encodes for the expression of the K5 capsule (15). Plasmid pBR322 (16) was used as a source of periplasmic beta -lactamase.

DNA Procedures-- Recombinant DNA procedures were performed by standard methods (17). Restriction endonucleases and DNA-modifying enzymes were purchased from Roche Molecular Biochemicals or Life Technologies, Inc., and used in accordance with the manufacturers' instructions. Pfu polymerase (cloned version) was purchased from Stratagene and used for amplifying DNA for cloning purposes. Splicing by Overlap Extension (18) was used to generate plasmid pTH56 in which the C-terminal 29 amino acids of KpsE are fused onto the C terminus of beta -lactamase. The primers 5'-GATCCGATCGATAACCCTGATAAATGCTTC-3' (SOE1A) plus 5'-CCAGCAGGCGATCAAATACCAATGCTTAATCAGTGA-3' (SOE1B) were used in PCR A; the primer 5'-TATTTGATCGCCTGCTGG-3' (SOE1C) was used with primer 5'-GTGCTCATCGATTTAGTCTCGGTGATCTTC-3' (SOE1D) in PCR B. The reaction products from PCRs A and B were combined in a ratio 1:1 and subjected to PCR C using the primers SOE1A and SOE1D. The reaction product from PCR C was digested with ClaI and inserted into the ClaI site in pACYC184.

K5 Bacteriophage Sensitivity Assay-- Assays were carried out as described previously (6).

Protein Analysis Procedures-- Cell lysates for SDS-PAGE were generated by resuspending the pellet from 1 ml of culture in 25 µl of SDS-PAGE sample buffer (19). Total cell proteins, ~50 µg per lane, were separated by SDS-PAGE according to the method of Laemmli (19). Proteins were either stained with Coomassie Blue or transferred to Immobilon-P polyvinylidene difluoride membrane (Millipore) by Western blotting as described by High et al. (20). Antibodies to beta -lactamase and chloramphenicol acetyltransferase were obtained from CP Laboratories; antiserum against KpsE was previously generated (21). Antibodies to purified KpsD were generated in the laboratory.2

Spheroplast Formation and Protease Digestion of Periplasmic Proteins-- Spheroplasts were prepared using the method of Rosenow et al. (21). Proteinase K was added at a final concentration of 500 µg ml-1, and spheroplasts were incubated at 37 °C for the appropriate time before the enzyme was inhibited by the addition of trichloroacetic acid to a final concentration of 20%. Carboxypeptidases A and B were added to final concentrations of 200 and 100 µg ml-1, respectively, and samples were incubated at 37 °C for 24 h before the enzyme was inhibited by the addition of 20% trichloroacetic acid. After trichloroacetic acid precipitation, the pellet was washed with acetone, dissolved in the appropriate volume of SDS-PAGE sample buffer, and analyzed by SDS-PAGE followed by Western blotting.

Cell Fractionation-- Cells were fractionated to give cytoplasmic membrane and outer membrane fractions using a modification of the method of Maier et al. (22). A 400-ml culture was grown to an A600 nm of 0.8 and cooled on ice. Cells were washed with 10 mM Tris-HCl, pH 8.0, and resuspended in 25 ml of 10 mM Tris-HCl, pH 8.0, 0.2 mM DTT, and 20 mg of DNase I were added. Bacteria were disrupted by passing them three times through a French pressure cell at 20,000 pounds/square inch or by sonication (5-s bursts with 10-s intervals for 3 min) on ice, and unbroken cells and debris were removed by centrifugation (7,000 × g for 15 min). The supernatant was subjected to centrifugation (80,000 × g for 1 h at 4 °C), and the resultant supernatant containing the cytoplasmic and periplasmic fractions was then decanted and saved for further analysis. The pellet containing the membrane fraction was washed with 20 ml of 10 mM Tris-HCl, pH 8.0, 0.2 mM DTT. The membranes were resuspended in ~1 ml of 10 mM Tris-HCl, pH 8.0, 0.2 mM DTT and layered on top of a two-step sucrose gradient (7.5 ml of 54% (w/v) sucrose on a cushion of 3 ml of 70% (w/v) sucrose in 10 mM Tris-HCl, pH 8.0). The gradient was centrifuged at 100,000 × g for 16-18 h at 6 °C, and fractions were collected from the sides of the tube with 23-gauge needles connected to 1-ml syringes and analyzed by SDS-PAGE followed by Western blotting. Following trichloroacetic acid precipitation the activity of the periplasmic enzyme alkaline phosphatase and the cytoplasmic enzyme malate dehydrogenase were assayed according to the method of de Maagd and Lugtenberg (23) to check for contamination of the membrane fractions with cytoplasmic and periplasmic enzymes. beta -NADH oxidase activity was measured in all the fractions from the gradient as a marker for the cytoplasmic membrane fractions (24), and the level of Kdo was used as a marker for the outer membrane (25). Membrane fractions were typically found to exhibit less than 2% total alkaline phosphatase and 3% total malate dehydrogenase activities indicating that they were essentially free of periplasmic and cytoplasmic contamination. Cytoplasmic plus periplasmic fractions were found to contain less than 4% of the total amount of Kdo and 5% total beta -NADH oxidase activity. Cytoplasmic membrane fractions were found to contain less than 2% of total amount of Kdo, and outer membrane fractions contained no more than 3% total beta -NADH oxidase activity, suggesting that these fractions were also predominantly free from contamination from other fractions of the gradient.

In VivoCross-linking with DSP-- Cross-linking with was carried out as described previously (26).


    RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Localization of the C Terminus of KpsE-- Previous attempts using beta -lactamase fusions as topology probes had established that KpsE was anchored to the cytoplasmic membrane via an N-terminal membrane-spanning domain located between residues 28 and 49 (21). However, these studies failed to assign an unequivocal location for the C terminus of KpsE and to establish whether the predicted C-terminal transmembrane domain located between residues 362 and 379 spanned the cytoplasmic membrane as predicted for MPA2 proteins (8). To determine whether the C terminus of KpsE is exposed in the periplasm, spheroplasts of the strain JM109[DE3] (pACYC184, pTH1), which expresses full-length KpsE protein (26), were incubated with either proteinase K or carboxypeptidases A and B. The digestion products were analyzed by Western blot using antisera to chloramphenicol acetyltransferase, beta -lactamase, KpsE, or EnvZ (Fig. 1). The periplasmic enzyme, beta -lactamase, was fully degraded by proteinase K and extensively degraded by carboxypeptidases A and B (Fig. 1). The extra bands of decreasing molecular weight seen in the presence of carboxypeptidases A and B can be explained by the slow release by these enzymes of aspartic acid, glutamic acid, and glycine residues, all of which occur in the C-terminal 30 amino acids of beta -lactamase. KpsE was also completely digested by proteinase K, confirming the presence of a large periplasmic loop (Fig. 1). The addition of carboxypeptidases A and B resulted in a limited digestion of KpsE, leading to a predominant truncated product ~1.0 kDa smaller than wild type KpsE (Fig. 1) confirming that the C terminus of KpsE is exposed to the periplasm. No degradation of the cytoplasmic enzyme chloramphenicol acetyltransferase could be detected (Fig. 1), confirming the integrity of the cytoplasmic membrane. To confirm the carboxypeptidase results, Western blot analysis using antisera against EnvZ was performed. EnvZ is an integral cytoplasmic membrane protein with cytoplasmic N and C termini with an extended periplasmic domain (27). No degradation of EnvZ was detectable following carboxypeptidase treatment (Fig. 1) confirming that the limited degradation of KpsE is due to the action of carboxypeptidase.



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Fig. 1.   Spheroplasts of strain JM109[DE3] (pACYC184, pTH1) were digested with either carboxypeptidases A and B (CP) or proteinase K (PK) as described under "Experimental Procedures" before Western blot analysis using antisera specific for chloramphenicol acetyltransferase (panel A), beta -lactamase (panel B), KpsE (panel C), or EnvZ (panel D). The arrows denote either chloramphenicol acetyltransferase (panel A), BlaM (panel B), KpsE (panel C), or EnvZ (panel D).-ve, spheroblasts were incubated without proteinase K and carboxypeptidases A and B.

To establish whether the hydrophobic C terminus of KpsE interacts with either the cytoplasmic or outer membrane, plasmid pTH56 was generated. This plasmid encodes the C-terminal 29 amino acids of KpsE fused in frame onto the C terminus of wild type beta -lactamase. Cells of strain PA360(pTH56) were fractionated by a two-step sucrose gradient to give cytoplasmic and outer membrane fractions and subjected to Western blot analysis (Fig. 2). As predicted, wild type beta -lactamase encoded by pBR322 was located in the soluble periplasmic fraction (Fig. 2), whereas in the case of pTH56, the presence of the C terminus of KpsE resulted in the localization of a significant amount of the beta -lactamase fusion protein to the cytoplasmic membrane (Fig. 2). The exposure of this fusion protein in the periplasm was confirmed by proteinase K treatment of spheroplasts prior to Western blot analysis. In both cases the proteins were digested by proteinase K, whereas no degradation of cytoplasmic chloramphenicol acetyltransferase was observed indicating that the cytoplasmic membranes of the spheroplasts were intact throughout the experiment (data not shown). The observation that plasmid pTH56 conferred resistance to ampicillin at levels greater than 100 µg ml-1 and that the fusion was sensitive to carboxypeptidase digestion (data not shown) confirms that the beta -lactamase fusion protein is functional and is anchored to the periplasmic face of the cytoplasmic membrane rather than being trapped in an unfolded state in the cytoplasmic membrane. Therefore, the C-terminal 29 amino acids of KpsE appear to be sufficient to localize beta -lactamase, the archetypal soluble periplasmic protein, to the cytoplasmic membrane, suggesting an intimate interaction between the C-terminal part of KpsE and the cytoplasmic membrane. The lack of other Kps proteins in strain PA360 would indicate that this association is not dependent on the interaction between the C terminus of KpsE and other Kps proteins.



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Fig. 2.   Western blot analysis of periplasmic (P) and cytoplasmic membrane (CM) fractions of strain PA360(pBR322) (panel A) and strain PA360(pTH56) (panel B) using antisera specific for beta -lactamase. The arrows denote either beta -lactamase (panel A) or the KpsE-beta -lactamase fusion protein (panel B).

Chemical Cross-linking of KpsE and KpsD-- To determine the interactions between KpsE and other proteins involved in capsule transport, whole cells of strain MS101 (Table I) were treated with the chemical cross-linking agent DSP. Strains with specific defects either in capsule export or in the production of several major proteins in the bacterial envelope (see below) were also analyzed in this way. The cross-linked proteins were separated by SDS-PAGE followed by Western blot analysis using antisera specific to KpsE and KpsD.


                              
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Table I
Strains used in this study

In MS101 four major cross-linked complexes were detected after treatment with DSP when KpsE-specific antiserum was used (Fig. 3, panel A). The most predominant cross-linked product had a molecular weight consistent with that of a dimer of KpsE. The three other cross-linked products had molecular weights of 110, 130 and in excess of 200 kDa (Fig. 3, panel A). Continued incubation with DSP resulted in higher molecular weight complexes that were difficult to resolve by SDS-PAGE (data not shown). The same pattern of cross-linking was seen in mutants lacking either KpsD, KpsM, KpsT, KpsS, or outer membrane proteins Pal, Lpp, OmpT, LamB, OmpF, OmpA, and OmpC (data not shown) suggesting that these proteins were not being cross-linked to KpsE. Cross-linking using cells expressing kpsE as the only gene from the capsular cluster resulted in the same pattern of cross-linking to that observed in MS101 (data not shown) indicating that the probable dimerization of KpsE is not dependent upon the expression of the polysaccharide molecule or on the presence of other Kps proteins. In all cases the cross-linking could be reversed by incubation in the presence of 50 mM DTT (data not shown). The ability of the purified KpsE protein to dimerize in vitro (Fig. 3, panel B) would suggest that the cross-linked complex at 86 kDa is likely to be dimeric KpsE.



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Fig. 3.   Panel A, Western blot analysis using KpsE-specific antisera of DSP cross-linked cells. Lane 1, PA360; lane 2, MS101. Panel B, Western blot analysis of purified KpsE. Lane 1, a Coomassie-stained gel of purified KpsE; lane 2, a Western blot of the same purified protein. Molecular masses are in kDa, and the arrows denote monomeric and dimeric KpsE.

When KpsD-specific antiserum was used to analyze the cross-linked products, a complex of 67 kDa was detected in MS101 but not in MSFE101 or MSCA102 (Fig. 4). Strain MSCA102 is an isogenic lpp mutant of MS101 that lacks the 7-kDa major outer membrane lipoprotein Lpp. However, Western blot analysis using Lpp-specific antisera failed to detect any cross-linking of Lpp to KpsD (data not shown). As such the 7-kDa protein that is cross-linked to KpsD cannot be Lpp, and its identity is as yet unknown.



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Fig. 4.   Western blot analysis using KpsD-specific antisera of whole cell lysates (panel A) and those from cells first treated with DSP (panel B). Lane 1, MSFE101; lane 2, MSCA102; lane 3, MS101. The cross-linked KpsD complex is shown by an arrow.

Proteinase K Sensitivity of KpsE and KpsD in the Presence and Absence of Other Proteins-- To demonstrate possible interactions between KpsE and KpsD, their proteinase K sensitivity in different mutant backgrounds was assessed by Western blot analysis following a time course proteolysis of E. coli spheroplasts. After 30 min of incubation with proteinase K, no proteolysis of KpsD was observed in spheroplasts from MS101 with limited proteolysis of KpsE after 30 min (Fig. 5, panels A and B). However, in MSFE101, where KpsE was absent from the bacterial envelope, KpsD was quickly degraded by proteinase K (Fig. 5, panel A). Likewise in strain MSTH101 (Table I) that lacks KpsD, KpsE was very sensitive to proteinase K (Fig. 5, panel B). In MSCA102, lacking Lpp, KpsD was reproducibly more sensitive to proteinase K digestion than in MS101 (Fig. 5, panel A), although compared with MSFE101, a significant proportion of KpsD was still resistant to proteolysis (Fig. 5, panel A). In contrast KpsE was rapidly degraded in MSCA102 (Fig. 5, panel B). In all cases there was no appreciable difference in the proteinase K sensitivity of periplasmic beta -lactamase (data not shown) confirming that the spheroplasts of each strain were equally permeable to proteinase K. 



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Fig. 5.   Western blot analysis using KpsD-specific antisera (panel A) or KpsE-specific antisera (panel B) following a time course digestion with proteinase K of spheroplasts. Samples were extracted at different time points during the digestion, precipitated with trichloroacetic acid, and subjected to SDS-PAGE and Western blot analysis.

Detection of KpsD in the Bacterial Envelope-- In the E. coli Tol-PAL system the periplasmic protein TolB has been shown to interact with the main peptidoglycan-associated outer membrane proteins Pal, OmpA, and Lpp (28). This interaction results in the localization of TolB both in the periplasm and the outer membrane (29). To establish if KpsD also interacts with the cytoplasmic and outer membranes, strains MS101, MSFE101, MSCA102, and MSCP101 were fractionated by a two-step sucrose gradient to give cytoplasmic and outer membrane fractions, which together with the soluble periplasmic fraction were subjected to Western blot analysis (Fig. 6). In MS101, KpsD was detected in all three fractions with the majority of the protein in the periplasm (Fig. 6). In MSFE101 lacking KpsE, KpsD was detected exclusively as soluble protein in the periplasm with no protein detectable in either membrane fraction (Fig. 6). In MSCA102 KpsD was distributed evenly in both membrane fractions, with no soluble KpsD in the periplasmic fraction (Fig. 6). In strain MSCP101 (Table I), which has all of the polysaccharide export machinery but makes no polysaccharide, KpsD was detected exclusively in the outer membrane fraction (Fig. 6).



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Fig. 6.   Western blot analysis using KpsD-specific antisera of periplasmic (P), cytoplasmic membrane (CM), and outer membrane (OM) fractions of strains MS101, MSFE101, MSCA102, and MSCP101.



    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The sensitivity of KpsE to carboxypeptidase demonstrates that its C terminus must be exposed in the periplasm and not be located in the cytoplasm as suggested previously for the MPA family of proteins (8). In addition, the observation that the C-terminal 29 amino acids of KpsE appear to be sufficient to localize beta -lactamase, the archetypal soluble periplasmic protein, to the cytoplasmic membrane suggests an intimate interaction between the C terminus of KpsE and the cytoplasmic membrane. The decreased sensitivity of KpsE to carboxypeptidase digestion when compared with beta -lactamase could be a consequence of the close association of the C terminus of KpsE with the periplasmic face of the cytoplasmic membrane, which makes the protein less accessible to the carboxypeptidase enzymes. A similar situation has been observed with TolR, the C terminus of which interacts with the periplasmic face of the cytoplasmic membrane and which has reduced sensitivity to carboxypeptidase digestion (30).

A helical wheel plot (31) of the C-terminal 20 amino acids of KpsE demonstrates the presence of a putative amphipathic alpha -helix with a segregation of hydrophobic and hydrophilic residues on either side of the helix (data not shown). It has been demonstrated that the interactions between PBP5 (32), PBP6 (33), and TolR (30, 34) and the periplasmic face of the cytoplasmic membrane are mediated by C-terminal amphipathic alpha -helices in which hydrophobic residues are buried in the membrane interior and the hydrophilic residues are located on the other side of the helix and interact with the aqueous environment (35). Therefore, it is likely that the interaction between the C terminus of KpsE and the periplasmic face of the cytoplasmic membrane may be also mediated by an amphipathic alpha -helix. Taken as a whole these data establish the topology of KpsE, the archetypal MPA protein, and remove any ambiguity about the location of the C termini of this family of proteins involved in the transport of capsular polysaccharides across the periplasmic space in Gram-negative bacteria.

The cross-linking data are consistent with KpsE functioning as a dimer, and the ability to dimerize was independent of the presence of other Kps proteins or the synthesis of capsular polysaccharide. The ability of KpsE to dimerize is likely to be mediated by a predicted coiled-coil structure present within KpsE (26). The cross-linking of KpsD to an unidentified 7-kDa protein is curious. The observation that this cross-linking was abolished in the absence of KpsE and Lpp suggested that KpsE and Lpp were required for this interaction. One must be cautious in assigning any specific role to Lpp in mediating this interaction, since mutations in lpp are known to have pleiotropic effects on the integrity of the periplasm (36), and this could explain the differences in the pattern of KpsD cross-linking in an lpp mutant. The failure to cross-link KpsE and KpsD to each other could reflect that these proteins interact very transiently and/or via the exported polysaccharide molecule, making it impossible to detect the interaction with DSP cross-linking. It has been demonstrated in type I protein secretion in Gram-negative bacteria that the interaction between components in the secretion pathway is mediated by the exported protein molecule (37, 38). Likewise, in the Tol system for the uptake of colicins, it has been suggested that the colicin itself may provide a bridge between its outer membrane receptor and periplasmic TolA protein (39). As such it is possible that a similar situation exists in the transport of group 2 capsular polysaccharides, whereby the exported polysaccharide molecule plays a key role in linking the different components of the transport pathway together. The alteration in the proteinase K sensitivity of KpsD and KpsE in the different mutant backgrounds probably reflects conformational changes in the KpsD and KpsE proteins as a result of the disruption of the transport process. This supports the notion that these proteins are likely to interact in the transport process.

Previously, KpsD has been assigned as a soluble periplasmic protein involved in group 2 polysaccharide transport (40). The identification of KpsD in both the cytoplasmic and outer membrane fractions suggests that the trans-periplasmic export of group 2 capsular polysaccharides requires the interaction of KpsD with both membranes as well as being present in the periplasm. One interpretation is that KpsD may cycle between the membranes moving polymer across the periplasm in the process. In this model KpsD would engage with the polysaccharide as it is exported across the cytoplasmic membrane by the KpsMT transporter before docking with the outer membrane and transporting the polysaccharide across the periplasm. Alternatively, some form of trans-periplasmic export complex consisting of KpsD may be formed. Which ever model is correct it would appear that KpsE is vital to allow the interactions between KpsD and the respective membranes, and it is possible that KpsE functions to bring the membranes together to facilitate this process. The lack of soluble periplasmic KpsD in MSCA102 suggests that in the absence of Lpp the export process is disrupted such that either a trans-periplasmic complex cannot form or KpsD cannot cycle between the two membranes. Whether this reflects a specific role for Lpp in the transport process or is a consequence of pleiotropic effects of the lpp mutation on the integrity of the periplasm are as yet unresolved. The lack of detectable KpsD in either the periplasmic or cytoplasmic membrane fractions of strain MSCP101, which is unable to synthesize K5 polysaccharide, indicates that there is an absolute requirement for the polysaccharide molecule to be engaged in the translocation process for the trans-periplasmic export pathway to be operational. In the absence of polysaccharide the KpsD protein is detected in the outer membrane fraction. These results support the notion that the polysaccharide molecule itself may mediate interactions between proteins involved in the different stages of the transport process and extends our earlier observations that there is a direct coupling between polysaccharide biosynthesis and export (5).

The translocation of polysaccharide across the outer membrane is still an area of conjecture. Unlike group 1 capsular polysaccharides, which have an outer membrane pore-forming protein (13), no such protein is encoded by group 2 capsule gene clusters. The observation that strain BL21[DE3]omp8(pPC6), which lacks OmpT, LamB, OmpF, OmpA and OmpC (41), is still able to make a K5 capsule3 would suggest that these major outer membrane proteins do not function in this capacity.

Taken as a whole the data presented in the paper provide the first information on the transport of group 2 capsular polysaccharides across the periplasm and the roles of KpsE and KpsD in this process. The identification of homologues of these Kps proteins in other Gram-negative pathogens (3) indicate these findings will be applicable in the transport of capsular polysaccharides in these other medically important micro-organisms.


    ACKNOWLEDGEMENTS

We thank Dr. J.-C. Lazzaroni for antiserum against Lpp and Dr. M. Inouye for antisera against EnvZ.


    FOOTNOTES

* This work was supported in part by the BBSRC, the Wellcome Trust, and the Lister Institute for Preventive Medicine.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Both authors contributed equally to this work.

§ Recipient of a Wellcome Travel fellowship.

Recipient of an MRC quota studentship.

|| Recipient of a Royal Thailand Student'ship.

** To whom correspondence should be addressed: 1.800 Stopford Bldg., School of Biological Sciences, University of Manchester, Oxford Rd., Manchester M13 9PT, UK. Tel.: 44 161 275 5601; Fax: 44 161 275 5656; E-mail: ISRobert@fs1.scg.man.ac.uk.

Published, JBC Papers in Press, November 14, 2000, DOI 10.1074/jbc.M008183200

2 C. Arrecubieta, unpublished results.

3 C. Arrecubieta and I. S. Roberts, unpublished results.


    ABBREVIATIONS

The abbreviations used are: Kdo, 2-keto-3-deoxyoctonate; MPA, membrane-periplasmic auxiliary protein; DSP, dithiobis(succinimidyl propionate); PAGE, polyacrylamide gel electrophoresis; DTT, dithiothreitol; PCR, polymerase chain reaction.


    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES


1. Whitfield, C., and Roberts, I. S. (1999) Mol. Microbiol. 31, 1307-1319[CrossRef][Medline] [Order article via Infotrieve]
2. Robbins, J. B., McCracken, G. H., Gotschlich, E. C., Orskov, I., and Hanson, L. A. (1974) N. Engl. J. Med. 90, 267-271
3. Roberts, I. S. (1996) Annu. Rev. Microbiol. 50, 285-315[CrossRef][Medline] [Order article via Infotrieve]
4. Bliss, J. M., and Silver, R. P. (1996) Mol. Microbiol. 21, 221-231[Medline] [Order article via Infotrieve]
5. Rigg, G. P., Barrett, B., and Roberts, I. S. (1998) Microbiology 144, 2905-2914[Abstract]
6. Roberts, I. S., Mountford, R., Hodge, R., Jann, K. B., and Boulnois, G. J. (1988) J. Bacteriol. 170, 1305-1310[Medline] [Order article via Infotrieve]
7. Bronner, D., Sieberth, V., Pazzani, C., Roberts, I. S., Boulnois, G. J., Jann, B., and Jann, K. (1993) J. Bacteriol. 175, 5984-5992[Abstract]
8. Paulsen, I. T., Beness, A. M., and Saier, M. H. J. (1997) Microbiology 143, 2685-2699[Abstract]
9. Smith, A. N., Boulnois, G. J., and Roberts, I. S. (1990) Mol. Microbiol. 4, 1863-1869[Medline] [Order article via Infotrieve]
10. Pavelka, M. S., Wright, L. F., and Silver, R. P. (1991) J. Bacteriol. 173, 4603-4610[Medline] [Order article via Infotrieve]
11. Pavelka, M. S., Hayes, S. F., and Silver, R. P. (1994) J. Biol. Chem. 269, 20149-20158[Abstract/Free Full Text]
12. Pigeon, R. P, and Silver, R. P. (1994) Mol. Microbiol. 140, 871-881
13. Drummelsmith, J., and Whitfield, C. (2000) EMBO J. 19, 57-66[Abstract/Free Full Text]
14. Silver, R. P., Aaronson, W., and Vann, W. F. (1987) J. Bacteriol. 169, 5489-5495[Medline] [Order article via Infotrieve]
15. Pazzani, C., Rosenow, C., Boulnois, G. J., Bronner, D., Jann, K., and Roberts, I. S. (1993) J. Bacteriol. 175, 5978-5983[Abstract]
16. Bolivar, F., Rodriguez, R. L., Greene, P. J., Betlach, M. C., Heyneker, H. L., Boyer, H. W., Crosa, J. H., and Falkow, S. (1977) Gene (Amst.) 2, 95-105[Medline] [Order article via Infotrieve]
17. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual , 2nd Ed. , Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
18. Horton, R. M. (1997) in PCR Cloning Protocols: From Molecular Cloning to Genetic Engineering (White, B. A., ed) , pp. 141-149, Humana Press Inc., Totowa, NJ
19. Laemmli, U. K. (1970) Nature 227, 680-685[Medline] [Order article via Infotrieve]
20. High, N. J., Hales, B. A., Jann, K., and Boulnois, G. J. (1988) Infect. Immun. 56, 513-517[Medline] [Order article via Infotrieve]
21. Rosenow, C., Esumeh, F., Roberts, I. S., and Jann, K. (1995) J. Bacteriol. 177, 1137-1143[Abstract]
22. Maier, C., Bremer, E., Schmid, A., and Benz, R. (1988) J. Biol. Chem. 263, 2493-2499[Abstract/Free Full Text]
23. de Maagd, R. A., and Lugtenberg, B. (1986) J. Bacteriol. 167, 1083-1085[Medline] [Order article via Infotrieve]
24. Dobrogozs, W. J. (1981) in Manual of Methods for General Microbiology (Gerhardt, P., ed) , pp. 365-392, American Society for Microbiology, Washington, D. C.
25. Karkhanis, Y. D., Zeltner, J. Y., Jackson, J. J., and Carlo, D. J. (1978) Anal. Biochem. 85, 595-601[Medline] [Order article via Infotrieve]
26. Hammarton, T. C. (1999) Analyses of the KpsE Protein of Escherichia coli K5Ph.D. thesis , University of Manchester, Manchester, UK
27. Hsing, W., Russo, F. D., Bernd, K. A., and Silhavy, T. J. (1998) J. Bacteriol. 180, 4538-4546[Abstract/Free Full Text]
28. Clavel, T., Germon, P., Vianney, A., Portalier, R., and Lazzaroni, J.-C. (1998) Mol. Microbiol. 29, 359-367[CrossRef][Medline] [Order article via Infotrieve]
29. Isnard, M., Rigal, A., Lazzaroni, J.-C., Lazdunski, C., and Lloubes, R. (1994) J. Bacteriol. 176, 6392-6396[Abstract]
30. Lazzaroni, J. C., Vianney, A., Popot, J. L., Benedetti, H., Samatey, F., Lazdunski, C., Portalier, R., and Geli, V. (1995) J. Mol. Biol. 246, 1-7[CrossRef][Medline] [Order article via Infotrieve]
31. Schiffer, M., and Edmundson, A. B. (1967) Biophys. J. 7, 121-135[Medline] [Order article via Infotrieve]
32. Jackson, M. E., and Pratt, J. M. (1988) Mol. Microbiol. 2, 563-568[Medline] [Order article via Infotrieve]
33. Phoenix, D. A., Peters, S. E., Ramzan, M. A., and Pratt, J. M. (1994) Microbiology 140, 73-77[Abstract]
34. Lazdunski, C., Bouveret, E., Rigal, A., Journe, L., Lloube, S. R., and Benedetti, H. (1998) J. Bacteriol. 180, 4993-5002[Free Full Text]
35. Gittins, J. R., Phoenix, D. A., and Pratt, J. M. (1994) FEMS Microbiol. Rev. 13, 1-12[Medline] [Order article via Infotrieve]
36. Choi, D.-S., Yamada, H., Mizuno, T., and Mizushima, S. (1986) J. Biol. Chem. 261, 8953-8957[Abstract/Free Full Text]
37. Letoffe, S., Delepelaire, P., and Wandersman, C. (1996) EMBO J. 15, 5804-5811[Abstract]
38. Thanabalu, T., Koronakis, E., Hughes, C., and Koronakis, V. (1998) EMBO J. 17, 6487-6496[Abstract/Free Full Text]
39. Benedetti, H., Lazdunski, C., and Lloubes, R. (1991) EMBO J. 10, 1989-1995[Abstract]
40. Wunder, D., Aaronson, W., Hayes, S., Bliss, J., and Silver, R. P. (1994) J. Bacteriol. 176, 4025-4033[Abstract]
41. Prilipov, A., Phale, P. S., Van Gelder, P., Rosenbusch, J. P., and Koebnik, R. (1998) FEMS Microbiol. Lett. 163, 65-72[CrossRef][Medline] [Order article via Infotrieve]
42. Stevens, M. P., Hanfling, P., Jann, B., Jann, K., and Roberts, I. S. (1994) FEMS Microbiol. Lett. 124, 93-98[CrossRef][Medline] [Order article via Infotrieve]
43. Petit, C., Rigg, G. P., Pazzani, C., Smith, A., Sieberth, V., Stevens, M., Boulnois, G., Jann, K., and Roberts, I. S. (1995) Mol. Microbiol. 17, 611-620[Medline] [Order article via Infotrieve]
44. Esumeh, F. (1996) Molecular Studies of the Escherichia coli K 5 Capsule Gene ClusterPh.D. thesis , University of Leicester, Leicester, UK
45. Yanisch-Perron, C., Vieira, J., and Messing, J. (1985) Gene (Amst.) 33, 103-119[CrossRef][Medline] [Order article via Infotrieve]


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