Mechanisms of hepatic very low density lipoprotein overproduction in insulin resistance. Evidence for enhanced lipoprotein assembly, reduced intracellular apoB degradation, and increased microsomal triglyceride transfer protein in a fructose-fed hamster model.

Changiz Taghibiglou, André Carpentier, Stephen C. Van Iderstine, Biao Chen, Debbie Rudy, Andrea Aiton, Gary F. Lewis, and Khosrow Adeli

Page 8423, Fig. 9: The wrong figure was published although the legend is correct. The correct figure is shown below.


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Fig. 9.   Intracellular distribution of nascent apoB-containing lipoproteins in microsomal lumen of control and fructose-fed hepatocytes. Cultured primary hamster hepatocytes were pulsed for 45 min with [35S]methionine, and the radioactivity was chased for 0 or 1 h. Labeled cells were then subjected to homogenization and fractionation of microsomes. Luminal lipoproteins were extracted from microsomes by carbonate treatment and were separated from the membrane fraction by centrifugation followed by fractionation on a sucrose-gradient. After centrifugation, gradient fractions were collected and immunoprecipitated with an anti-hamster apoB antibody. Immunoprecipitates were analyzed by SDS-PAGE and fluorography, and apoB radioactivity was quantitated by cutting and scintillation counting of the apoB-100 band. A, luminal lipoproteins in control hepatocytes at 0 and 1 h chase; B, luminal lipoproteins in fructose-fed hepatocytes at 0 and 1 h chase.

Data Interpretation: The interpretation of the data in Fig. 9 does not significantly change with this correction. However, an important observation also reported previously should be emphasized further. As observed in the corrected Fig. 9, microsomes from control cells had a significant amount of dense apoB-containing lipoproteins (HDL density), at both 0 and 1 h, which were absent in hepatocytes from fructose-fed hamsters. The absence of HDL-dense apoB particles suggests the stimulated state of VLDL assembly and secretion in hepatocytes of fructose-fed hamsters.





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