Since publication of this paper, a
couple of relevant matters have come to light. Our inability to clone a
glycogenin-2 cDNA or gene from rodent tissues prompted us to re-examine
the two pieces of evidence presented in this paper for the existence of glycogenin-2 in the rat. First, we had reported the presence of glycogenin-2 in rat H4IIEC3 cells as judged by Western blotting. We
have now found that the cells used were of human origin as judged by
the karyotype. Furthermore, when we reacquired H4IIEC3 cells from the
ATCC, we could not detect glycogenin-2 by Western blotting or by PCR. A
misidentification of the cell line had occurred, and therefore all
reference in this paper to H4EIIC3 cells must be reinterpreted as
referring to a human cell line of unknown origin. Second, efforts to
reproduce detection of glycogenin-2 directly in rat cell extracts (Fig.
6A) have not been successful. These findings contribute to
our current hypothesis that rodents do not have a glycogenin-2 gene.
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