From the Departments of Pathophysiology and
§ Medical Chemistry, University of Vienna, A-1090 Vienna,
Austria and the Division of Biochemistry, Institute of Medical
Biochemistry, Vienna Biocenter, University of Vienna,
A-1030 Vienna, Austria
Received for publication, May 31, 2000, and in revised form, November 8, 2000
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ABSTRACT |
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Minor group human rhinoviruses (exemplified by
human rhinovirus serotype 2 (HRV2)) use members of the low density
lipoprotein receptor family for cell entry; all these receptors possess
clathrin-coated pit localization signals. Viral infection should thus
be inhibited under conditions of impaired clathrin-mediated
endocytosis. However, Madshus et al. reported an increase
in the cytopathic effect of HRV2 infection in HEp-2 cells upon
suppression of clathrin-dependent endocytosis by hypotonic
shock and potassium depletion (Madshus, I. H., Sandvig, K.,
Olsnes, S., and van Deurs, B. (1987) J. Cell. Physiol.
131, 14-22.) To resolve this apparent contradiction, we investigated
the binding, internalization, conformational changes, and productive
uncoating of HRV2 in HeLa cells subjected to hypotonic shock and
potassium depletion. This treatment led to an increase in HRV2 binding,
with internalization being barely affected. The generation of
C-antigenic particles requiring pH About 50% of all mild infections of the upper respiratory tract,
generally recognized as common colds, are caused by human rhinoviruses.
These small RNA-containing viruses constitute a large genus within the
family Picornaviridae. The >100 serotypes are divided into
two groups based on their receptor specificity (1, 2); major group
viruses (91 serotypes) have access to the host cell by binding to
intercellular adhesion molecule-1 (ICAM-1/CD54)1 (3-5),
whereas minor group viruses (10 serotypes) bind to members of the low
density lipoprotein receptor (LDLR) family (6, 7). Internalization into
bona fide endosomes has been demonstrated for the minor
group virus HRV2 and the major group virus HRV14 (8). All receptors of
the LDLR family, including LDLR, LDLR-related protein (LRP), and very
low density lipoprotein receptor (VLDLR), contain tyrosine- and
dileucine-based internalization signals in their cytoplasmic tails that
direct them into clathrin-coated pits (9). At least for LDLR, it has
been explicitly shown that ligand internalization depends on functional
clathrin coats (10). Although specific binding of HRV2 to these
receptors is well characterized (6, 7, 11, 12), it is so far unknown
whether any of them is preferentially used by HRV2 for productive
infection of HeLa cells.
Soon after cell entry, native infectious viral particles undergo a
conformational change induced by the low pH ( Although the endocytic subcompartment where HRV2 uncoating takes place
has been characterized, the initial pathway of HRV2 entry is not
completely resolved. All receptors that have been demonstrated to
mediate HRV2 attachment contain clathrin localization signals, and
viral infection via clathrin-dependent endocytosis would
appear most likely. However, in 1987, when the nature of the minor
group rhinovirus receptor was unknown, Madshus et al. (18)
reported that inhibition of clathrin-dependent endocytosis by K+ depletion results in an increase in the cytopathic
effect of HRV2 in human epidermoid HEp-2 cells, a finding that is
strongly suggestive of clathrin-independent viral internalization.
HeLa cells stably transfected with a dominant-negative mutant of
dynamin-1 (dynaminK44A) have been generated (19). The
mutation results in loss of the GTPase activity, inhibition of
clathrin-coated pit constriction, and arrest of
clathrin-dependent endocytosis. Using these cells, it was
recently shown that productive infection by the major group virus HRV14
is dependent on functional dynamin (20), although the receptor for the
major group of human rhinoviruses, ICAM-1, lacks internalization
signals (21). We used this genetic defect in dynamin to exclude
nonspecific ion concentration effects as occur under
K+ depletion to obtain internalization data for HRV2.
These seemingly irreconcilable data on the entry of rhinoviruses and,
in particular, on that of the minor group virus HRV2 prompted us to
investigate in detail the early events in viral infection. In
particular, we dissected the effect of hypotonic shock/K+
depletion on the binding, internalization, conformational changes, and
productive uncoating of HRV2. The results thereof, together with data
obtained with cells expressing mutant dynamin-1, demonstrate that viral
internalization can occur via LRP and/or VLDLR by a clathrin-independent pathway.
Chemicals Buffer Solutions--
The following buffers were used: isotonic
KCl-free buffer (140 mM NaCl, 20 mM HEPES-NaOH
(pH 7.4), 1 mM CaCl2, 1 mM
MgCl2, 1 mg/ml glucose, and 0.5% bovine serum albumin),
isotonic KCl buffer (10 mM KCl, 130 mM NaCl, 20 mM HEPES-NaOH (pH 7.4), 1 mM CaCl2,
1 mM MgCl2, 1 mg/ml glucose, and 0.5% bovine
serum albumin), hypotonic medium (serum-free Leibovitz L-15 medium or
DMEM diluted 1:1 with water), PBS2+ (PBS with 1 mM CaCl2 and 1 mM
MgCl2), lysis buffer (100 mM Tris-HCl (pH 7.4),
100 mM NaCl, and 0.5% Triton X-100), and radioimmune precipitation assay (RIPA) buffer (50 mM Tris-HCl (pH 7.4),
150 mM NaCl, 1 mM EDTA, 1% sodium
deoxycholate, 0.1% SDS, and 1% Triton X-100) (27).
Cell Culture and Viral Propagation--
HeLa cells (Wisconsin
strain, kindly provided by R. Rueckert, University of Wisconsin) were
grown as monolayers in Eagle's minimal essential medium (MEM) (Life
Technologies, Inc.) containing heat-inactivated 10% fetal calf serum
(FCS), 2 mM L-glutamine, and 1% nonessential
amino acids or as a suspension culture in Joklik's DMEM (Life
Technologies, Inc.) supplemented with heat-inactivated 7% horse serum.
For K+ depletion, HeLa cells (2.5 × 106/plate) were grown in 12-well plates. HeLa cells stably
expressing dynaminK44A or dynaminwt were kindly
supplied by Dr. S. L. Schmid (Department of Cell Biology, Scripps
Research Institute, La Jolla, CA). The cells were cultivated in
DMEM with high glucose, L-glutamine, and sodium pyruvate
(Life Technologies, Inc.) supplemented with heat-inactivated 10% FCS,
400 µg/ml G418 (Life Technologies, Inc.), 200 ng/ml puromycin, and 1 µg/ml tetracycline. Cells were dislodged from subconfluent cultures
with trypsin/EDTA, and 1.5 or 3.5 × 105 cells/well
were plated on 12- or 6-well plates (Falcon), respectively, in the
absence of tetracycline for 48 h (induction) before use; at that
time, the cells finally were 60% confluent. HRV2 was propagated, labeled with [35S]cysteine/methionine, and purified as
described (28).
K+ Depletion in HeLa Cells following Hypotonic
Shock--
HeLa cells (Wisconsin strain, a subclone selected for
increased capacity to replicate human rhinoviruses) were incubated in serum-free Leibovitz L-15 medium for 30 min at 37 °C and
K+-depleted as described (10). Briefly, cells were
incubated in hypotonic medium for 5 min at 37 °C, followed by
incubation in isotonic KCl-free buffer for 30 min at 37 °C. For
control incubations, cells were maintained in Leibovitz L-15 medium as
indicated above, and all incubations were carried out in isotonic KCl buffer.
FITC-Transferrin Binding and Internalization--
Control or
K+-depleted HeLa cells were cooled to 4 °C and incubated
with 50 µg/ml FITC-transferrin in K+-containing or
K+-free buffer for 60 min at 4 °C. Cells were washed
with PBS2+ and lysed in 0.5 ml of lysis buffer, and cell
debris were removed by centrifugation. The FITC fluorescence in the
supernatant was measured in a CytoFluor 2300 (Millipore Corp.) using a
standard filter set (excitation, 485 nm (20-nm slit width);
emission, 530 nm (25-nm slit width)). After subtraction of the
background fluorescence (unlabeled cells), the amount of
cell-associated FITC-transferrin was calculated using a calibration
curve derived from serial dilutions of FITC-transferrin in lysis buffer.
To determine the internalization of plasma membrane-bound transferrin,
an aliquot of the cells was washed with PBS2+ and incubated
in the respective K+-containing or
K+-free buffer at 37 °C for 20 min. To halt
internalization, cells were again cooled, and membrane-bound
transferrin was removed by washing the cells with 25 mM
acetic acid containing 150 mM NaCl for 15 min at 4 °C,
followed by washing with ice-cold PBS2+ for 15 min. This
treatment completely removes plasma membrane-bound transferrin (23).
After cell lysis, internalized transferrin was determined as described above.
Fluid-phase Uptake--
Control or K+-depleted cells
were incubated with 5 mg/ml FITC-labeled dextran (70 kDa) dissolved in
K+-containing or K+-free buffer, respectively,
for 20 min at 37 °C. Excess FITC-dextran was removed by washing the
cells three times with ice-cold PBS2+. Cells were lysed in
0.5 ml of lysis buffer, and cell debris were removed by centrifugation.
The FITC fluorescence in the supernatant was measured as described for
FITC-transferrin.
Viral Binding and Internalization--
To determine viral
binding, control or K+-depleted cells grown in 12-well
plates were incubated in isotonic K+-containing or
K+-free buffer, respectively, in the presence of ~25 × 103 cpm/well 35S-labeled HRV2 for 1 h
at 4 °C. Cells were then washed with the same ice-cold buffers, and
cell-associated radioactivity was measured in cell lysates using a
liquid scintillation counter. Nonspecific binding was determined by
incubation in the respective buffer in the presence of 10 mM NaEDTA (pH 7.4) for 1 h at 4 °C.
The efficiency of removal of HRV2 bound to the cell membrane at 4 °C
was assessed using the following methods: (i) extensive washing with
K+-free buffer without CaCl2 and
MgCl2, but supplemented with 10 mM NaEDTA; (ii)
incubation with 20 mM MES titrated with tetramethylammonium hydroxide to pH 5.0, 10 mM KCl, 140 mM NaCl, 1 mM CaCl2, 1 mM MgCl2,
and 1 mg/ml glucose for 2 × 5 min, followed by washing with
ice-cold PBS2+; (iii) incubation with 2.5% trypsin (100 µl/well) for 60 min (as cells were detached from the plastic dishes
by the latter treatment, they were collected and washed with
PBS2+ by low speed centrifugation); and (iv) determination
of 35S-labeled HRV2 remaining accessible at the cell
surface after immunoprecipitation by incubation with the virus-specific
monoclonal antibody (mAb) 8F5 (29). For Method iv, cells were incubated with 200 µl/well 8F5 hybridoma supernatant for 2 h, washed with PBS2+, and lysed in RIPA buffer (500 µl/well).
Virus-antibody complexes were then precipitated by addition of 30 µl
of a 10% (w/v) IgG-sorb suspension for 3 h at room temperature.
Immunocomplexes were pelleted at 13,000 × g for 5 min.
One-hundred µl of 8F5 was then added to the supernatant; and
incubation, immunocomplex formation, and pelleting were repeated.
Pellets were washed twice with RIPA buffer and twice with PBS and
suspended in 10 volumes of scintillation mixture (Ready-Value, Beckman
Instruments) for counting. Finally, for viral uptake
experiments, control or K+-depleted cells were incubated
with 25 × 103 cpm 35S-labeled HRV2 for 20 min at 34 °C in the respective buffer; cells were washed with
ice-cold PBS containing 10 mM NaEDTA and pelleted; and
cell-associated radioactivity was determined in the cell lysates as
described above.
Immunoprecipitation of HRV2--
S. aureus mAb 2G2
and S. aureus rabbit anti-HRV2 antiserum immunocomplexes
were made as described (30). After incubation of the cells with HRV2,
cell supernatants and cells were processed separately. Cell pellets
were lysed in 300 µl of RIPA buffer for 15 min at 4 °C. Cell
debris were removed, and C-antigenic particles were recovered by
incubation with the mAb 2G2 immunocomplexes. D-antigenic particles
remaining in the supernatant were then precipitated with rabbit
antiserum against HRV2 and S. aureus cells. Precipitates were washed twice with RIPA buffer and twice with PBS and then analyzed
on SDS-12% polyacrylamide minigels. Gels were soaked in 1 M sodium salicylate for 30 min, dried, and exposed to x-ray film. Quantification was by laser densitometry (Ultrascan XL, Amersham
Pharmacia Biotech) of the fluorographs.
Restoration of Cellular Protein Synthesis upon Hypotonic Shock
and K+ Depletion--
All incubations were carried out at
34 °C. Control or K+-depleted HeLa cells (1 × 106 cells/ml for each time point) were incubated in
isotonic K+-containing or K+-free buffer,
respectively. After 20 min, cells were resuspended in the respective
buffers containing 200 nM bafilomycin A1 to dissipate the low intravesicular pH. After 40 min, cells were transferred to methionine-free MEM with 2% FCS (containing bafilomycin A1) and incubated further for 4, 6, 8, and 17 h.
Cellular proteins were labeled with 1 µCi/ml
[35S]methionine for 1 h (10). Cells were washed with
ice-cold PBS, and incorporated [35S]methionine was
measured after protein precipitation with 10% trichloroacetic acid.
Protein synthesis in K+-depleted cells at a given
time point was related to that in the control cells, which was set to
100%. The efficiency of K+ depletion was always verified
by quantitation of FITC-transferrin internalization (see above).
Viral Protein Synthesis--
All incubations were at 34 °C.
Infection of control or K+-depleted HeLa cells (1 × 106 cells/ml) was carried out in K+-containing
or K+-free buffer, respectively, for each time point. HRV2
was added at a multiplicity of infection (m.o.i.) of 100 for 20 min. To halt uncoating of input virus, cells were transferred to fresh buffers
supplemented with 200 nM bafilomycin A1 and
further incubated for 40 min. The drug was also present during all
subsequent incubations. To allow for recovery of protein synthesis,
which is reduced under K+-depleted conditions, control and
K+-depleted cells were transferred to methionine-free MEM
with heat-inactivated 2% FCS. Seven h post-infection, 20 µCi/ml
[S35]methionine was added. Seventeen hours
post-infection, the cells were pelleted and lysed in 300 µl of RIPA
buffer. Virus was immunoprecipitated with anti-HRV2 antiserum and
analyzed by SDS-polyacrylamide gel electrophoresis. The intensity of
the viral protein bands was determined from fluorographs by laser densitometry.
Fluorescence Microscopy--
HeLa cells expressing HA-tagged
dynaminK44A or dynaminwt were plated at low
density on 8-well chamber glass slides and cultivated in
tetracycline-free DMEM for 48 h prior to the experiment.
Inhibition of transferrin internalization into dynaminK44A
cells was verified by fluorescence microscopy. Cells were depleted of
endogenous transferrin by incubation in serum-free DMEM for 30 min at
37 °C. They were then incubated in fresh medium containing 50 µg/ml FITC-transferrin for 20 min at 37 °C and cooled. Plasma membrane-bound transferrin was removed by acid wash (see above), followed by a wash with PBS, fixed with 4% paraformaldehyde for 1 h at room temperature, quenched with 50 mM
NH4Cl, permeabilized with 0.05% saponin, and incubated
with rhodamine-labeled anti-HA monoclonal antibody (1:80 dilution). For
HRV2 internalization, cells were then incubated with HRV2 at a m.o.i.
of 100 in DMEM for 20 min at 34 °C. This high m.o.i. was found to be
necessary for HRV2 detection. To study the influence of GST-RAP on HRV2 internalization, cells were preincubated in DMEM containing GST-RAP (100 µg/ml) at 4 °C for 1 h. GST-RAP was also present during
HRV2 internalization. Cells were then transferred to 4 °C and washed with ice-cold PBS2+, and plasma membrane-bound virus was
masked by incubating the cells with rabbit anti-HRV2 antiserum
(preadsorbed with non-infected cells and diluted 1:10) for 1 h at
4 °C. Cells were washed with PBS2+, fixed with 4%
paraformaldehyde in PBS for 1 h at room temperature, quenched with
50 mM NH4Cl, and permeabilized with 0.05%
saponin. First, internalized D- and C-antigenic HRV2 was detected by
indirect immunofluorescence using mAb 8F5 at 6 µg/ml and Alexa
488-labeled goat anti-mouse antibody (1:500). Then, cells
overexpressing HA-dynaminwt and HA-dynaminK44A
were identified using a rhodamine-labeled monoclonal antibody specific
for the HA epitope. It should be mentioned that a punctate fluorescence
appeared in cells devoid of HA-dynamin expression, but with
internalized HRV2. This resulted from interaction of the mouse anti-HA
monoclonal antibody with residual binding sites present on the
secondary anti-mouse antibody used to detect mAb 8F5 attachment to
HRV2. Various attempts to block these free binding sites failed, but
the appearance of the staining pattern allowed a clear differentiation.
Cells were mounted in Moviol and viewed with a Zeiss Axioskop 2 microscope. Digital images were processed with the Zeiss KS400 imaging
program. Confocal images were taken with a Leica TCS NT Universal
confocal microscope.
Determination of the Fraction of
DynaminK44A-expressing Cells--
Impaired
clathrin-dependent internalization directly correlates with
the number of transferrin receptors at the plasma membrane. The
percentage of dynaminK44A-expressing cells was thus
determined via fluorescence-activated cell sorting analysis of
FITC-transferrin binding (31). Induced cells were incubated for 30 min
in serum-free DMEM, cooled to 4 °C, and incubated with 50 µg/ml
FITC-transferrin in DMEM for 1 h. Excess transferrin was removed
by washing; cells were scraped off the dish, resuspended in PBS, and
immediately analyzed by flow cytometry. From the two peaks seen, the
one with higher fluorescence intensity was taken to correspond to those
cells that had a higher density of transferrin receptors as a result of
dynaminK44A expression. From these measurements, it was
inferred that 70% of the induced cells (subpopulation with high FITC
intensity) were positive for exogenous dynamin expression.
Endosome Labeling for Flow Cytometry--
To investigate the
effect of K+ depletion on endosomal pH, control or
K+-depleted HeLa cells were incubated in
K+-containing or K+-free buffer, respectively,
containing 4 mg/ml FITC-dextran (70 kDa) and 1 mg/ml Cy5-dextran (70 kDa) for 20 min at 37 °C. Cells were washed three times with
ice-cold PBS and analyzed immediately by flow cytometry (32). To
measure the endosomal pH in dynaminwt and
dynaminK44A cells, they were incubated at 37 °C in DMEM
containing 4 mg/ml FITC-dextran (70 kDa) and 1 mg/ml Cy5-dextran (70 kDa) either for 20 min or for 5 min, followed by incubation in
dextran-free DMEM for 15, 55, and 115 min, respectively. Cells were
washed three times with ice-cold PBS and analyzed immediately by flow cytometry.
Generation of a pH-standard Curve for Flow
Cytometry--
Standard buffers with pH values between 5.0 and 7.5 were obtained by mixing 50 mM HEPES with 50 mM
MES, both containing 50 mM NaCl, 30 mM ammonium
acetate, and 40 mM sodium azide. A pH calibration curve of
internalized FITC/Cy5-dextran was generated using aliquots of the
labeled cells (24). Cells were divided into eight aliquots, pelleted,
resuspended in the various buffers, and equilibrated at 4 °C for 10 min prior to analysis. Under these conditions, cells are depleted of
endogenous ATP; vacuolar ATPases do not function; and equilibration of
intravesicular and external media is accomplished by weak acid-base
buffers (24, 33).
Flow Cytometry and Calculation of Endosomal pH--
A dual-laser
FACSCalibur (Becton Dickinson Immunocytometry Systems) equipped with
argon ion and red diode lasers was used. FITC fluorescence was
determined at 488 nm using a 530-nm band-pass filter (30-nm bandwidth).
Cy5 fluorescence (635 nm) was determined using a 661-nm band-pass
filter (16-nm bandwidth). Each sample was measured eight times; samples
in standard buffers were determined in duplicate. The mean values of
FITC fluorescence and Cy5 fluorescence were calculated for each sample,
and the autofluorescence of unlabeled samples was subtracted. The ratio
of FITC to Cy5 was determined, and the mean pH of the labeled endocytic
compartments was calculated using the pH-standard curve (24).
Internalization of FITC-Transferrin, 125I-Labeled
Rabbit
Since we intended to use the fluid-phase marker FITC-dextran for
intravesicular pH measurements, the influence of hypotonic shock/K+ depletion on its uptake was also investigated.
Inhibition of clathrin-dependent endocytosis has been
reported to either reduce or not affect fluid-phase endocytosis
in different cell types (37, 52). Cells were incubated with
FITC-dextran for 20 min at 37 °C, and cell-associated fluorescence
was determined. As depicted in Fig. 1B, fluid-phase
uptake into HeLa cells was reduced in K+-depleted cells to
~50% of the control, a value that compares well with that previously
determined for rat fibroblasts (37).
Removal of Plasma Membrane-bound HRV2 Is Not
Quantitative--
Determination of HRV2 uptake requires removal of
cell surface-attached virus. Therefore, the influence of K+
depletion on viral binding to the plasma membrane of HeLa cells was
determined. Cells were incubated with
[35S]methionine-labeled virus at 4 °C for 1 h,
washed with PBS2+, and lysed; and cell-associated
radioactivity was measured. HRV2 binding to K+-depleted
cells was always found to be increased compared with control cells.
However, it largely varied in five individual experiments using
different viral preparations (between 120 and 300% of the control
values). The increased binding might be caused by plasma membrane
accumulation of the receptors resulting from impaired internalization
or by an increase in receptor affinity as reported for binding of low
density lipoprotein to LDLR (10). Next, the efficiency of various
treatments to remove plasma membrane-bound virus was examined. Cells
were incubated with 35S-labeled HRV2 as described above and
washed with PBS2+, and total cell-associated virus was
determined from an aliquot; this value was taken as 100%. The
remainder of the cells were subjected to the procedures listed in Table
I. After each treatment, the cells were
washed with PBS2+, and cell-associated radioactivity was
measured by scintillation counting. As EDTA specifically inhibits HRV2
attachment (38), nonspecific binding was determined in the presence of
10 mM EDTA. When present during incubation of the cells
with virus, EDTA indeed strongly reduced surface binding (only 10 and
3% bound to control and K+-depleted cells, respectively);
however, it failed to efficiently remove virus from the cells (with 59 and 38% of the virus remaining bound). The effect of washing with
sodium acetate buffer adjusted to pH 5.0 was similar, and even trypsin
at 2.5% was not able to quantitatively remove surface-bound
virus.
At 4 °C, plasma membrane-bound virus should also remain accessible
to antibodies. We thus tried to distinguish bound virus from
internalized virus with the aid of mAb 8F5. This neutralizing antibody
binds bivalently to a linear epitope on the viral capsid protein VP2
and does not aggregate (29). Similarly to a polyclonal antiserum, it
also recognizes 135 S and 80 S particles as well as denatured VP2;
thus, it binds to all possible conformations of the virus. Cells with
35S-labeled HRV2 bound at 4 °C were incubated with mAb
8F5, and virus-antibody complexes were recovered by S. aureus aided immunoprecipitation. Scintillation counting revealed
that only 54 and 32% of the virus bound to control and
K+-depleted cells, respectively, were recovered in the
immunoprecipitates. Taken together, these experiments indicate that
plasma membrane-bound virus cannot be quantitatively removed or exactly
determined by either of these techniques and suggest that some of the
virus might be rendered inaccessible even at 4 °C by a so far
unknown mechanism. This is reminiscent of an earlier observation by
Lonberg-Holm and Whiteley (38) that HRV2 undergoes a transition
from weak binding to strong binding even at 4 °C.
Effect of Inhibition of Clathrin-dependent Endocytosis
on HRV2 Internalization--
As washing with EDTA removed about half
of the plasma membrane-bound virus from control and
K+-depleted cells and, at the same time, was less damaging
to the cells than incubation with trypsin or low pH buffer (see Table I), this treatment was chosen for the study of HRV2 internalization. Control and K+-depleted cells were incubated with
35S-labeled HRV2 for 20 min at 34 °C (the optimal growth
temperature of human rhinoviruses) and washed with the respective
buffers at 4 °C, and cell-associated virus was determined after EDTA
treatment. Under these conditions, 3577 ± 293 cpm/well was
associated with control cells, and 4590 ± 1371 cpm/well
(mean ± S.D. of three experiments done in triplicate) was
associated with K+-depleted cells. Thus, 28% more virus
was associated with K+-depleted cells compared with control
cells. As ~50% of the plasma membrane-bound virus can be removed by
EDTA treatment (Table I), this difference presumably reflects only the
elevated levels of HRV2 binding to the plasma membrane of
K+-depleted cells (see above). Thus, internalization
appears to proceed almost undisturbed in K+-depleted cells,
suggesting clathrin-independent viral uptake.
Low pH-mediated Conversion to C-antigenic Particles Is Decreased in
K+-depleted Cells--
After HRV2 internalization,
structural modifications of the capsid are exclusively triggered by pH
The pH of Endocytic Compartments Is Increased upon K+
Depletion--
To determine the endosomal pH, cells were incubated
with FITC-dextran (pH-sensitive) and Cy5-dextran (pH-insensitive)
fluid-phase markers for 20 min at 37 °C. After cooling, cells were
washed and immediately analyzed by flow cytometry. The average pH of all endosomal compartments labeled under these conditions was then
calculated from the ratio of the FITC and Cy5 fluorescence intensities
based on a pH-standard curve (Fig.
3A). As shown in Fig.
3B, the average pH of dextran-labeled compartments was found to be 6.2 in control cells, whereas cells subjected to K+
depletion showed an average pH of 6.6. Thus, hypotonic
shock/K+ depletion results not only in dissociation of
clathrin coats from the plasma membrane, but also in a severe reduction
of endosomal acidification by ~0.4 pH units. Since these values
represent an average of all compartments labeled under these particular
conditions, at least some of them must acidify to pH Is Productive Uncoating Affected by Hypotonic Shock and
K+ Depletion?--
Viral infection depends on release of
the RNA from the capsid and its efficient transfer from endosomes into
the cytoplasm (productive uncoating). Therefore, we asked whether
K+ depletion has any effect on productive uncoating. Native
virus is converted from 150 S to 135 S particles upon release of the innermost capsid protein VP4. Additional release of the RNA results in
80 S particles; both particles are C-antigenic and can thus not be
distinguished by mAb 2G2 immunoprecipitation. Furthermore, generation
of C-antigen is not necessarily correlated with productive uncoating;
nevertheless, it is a sine qua non condition. Consequently, de novo synthesis of viral proteins might be taken as a
measure for productive uncoating. However, hypotonic
shock/K+ depletion interferes with cellular protein
synthesis (40), which can be restored upon returning the cells to
K+-containing medium (10). Therefore, we first determined
the time required for protein synthesis to recover from the effects of
K+ depletion. Following the procedure detailed under
"Experimental Procedures," protein synthesis in
K+-depleted cells was found to be initially reduced to half
of the control values, but recovered within 6 h. It then remained
constant for up to 17 h (data not shown). Based on these results,
the following protocol for the analysis of viral protein synthesis was
established. Control and K+-depleted cells were challenged
with HRV2 at a m.o.i. of 100 for 20 min at 34 °C in the respective
buffers. To halt virus uncoating after return of the cells to normal
medium (MEM), they were washed and resuspended in buffers containing
200 nM bafilomycin A1 to elevate the endosomal
pH to neutrality (13). After incubation for 40 min, cells were
transferred into methionine-free MEM containing 2% FCS and bafilomycin
A1 to allow for regeneration of protein synthesis (see
above). Six h later, [35S]methionine was added, and
incubation was continued for a further 10 h (for experimental
setup, see Fig. 4). Cells were washed and lysed, and viral proteins were immunoprecipitated with anti-HRV2 antiserum. Proteins were separated by SDS-polyacrylamide gel
electrophoresis, and incorporation of radioactivity was quantified by
densitometry of the bands corresponding to VP1, VP2, and VP3 as seen on
the x-ray film upon fluorography. As depicted in Fig. 4, synthesis of
viral proteins in K+-depleted cells appeared to be slightly
reduced compared with control cells. Densitometry of gels obtained from
five independent experiments revealed a mean reduction in protein
synthesis to 80% of the control value. This reduction in de
novo viral protein synthesis has to be seen in relation to the
reduction in C-antigenic particles in K+-depleted cells
(Fig. 2). Since the conformational alteration from native virus to
C-antigenic particles is a prerequisite for uncoating and subsequent
infection, a reduction in these particles is expected to result in a
similar decrease in viral protein synthesis. However, the diminution of
C-antigenic particles in K+-depleted cells to 11% of the
control value (Fig. 2) does not parallel the observed reduction in
viral protein synthesis (80%). Thus, additional effects must account
for this apparently augmented transfer of the genomic RNA into the
cytoplasm in K+-depleted cells.
Taken together, depletion of cellular K+ not only leads to
inhibition of clathrin-dependent endocytosis, but also
differently affects HRV2 binding, endocytosis, structural modification,
and productive uncoating. Therefore, viral production cannot be
directly correlated with the extent of clathrin-dependent
endocytosis after inhibition by hypotonic shock and K+ depletion.
The pH of Endocytic Compartments Is Increased in HeLa Cells
Overexpressing DynaminK44A--
To avoid possible
artifacts caused by the alteration of ion fluxes or membrane potential
upon hypotonic shock and K+ depletion, we analyzed HRV2
entry by an approach similar to that recently chosen by DeTulleo
and Kirchhausen (20). These workers investigated the requirement of
functional clathrin-dependent endocytosis for infection by
several enveloped and non-enveloped viruses. HeLa cell lines expressing
either exogenous dynaminwt or dynaminK44A both
carrying a HA tag have been established previously (19). Although
overexpression of dynaminwt has no effect on
internalization via coated pits and coated vesicles, coated pits fail
to become constricted, and budding of clathrin-coated vesicles is
inhibited upon induction of dynaminK44A overexpression. As
a consequence, clathrin-dependent endocytosis is blocked.
It is known that exogenous dynamin is not homogeneously expressed
within the cell population (41), and only those cells (70-80%) that
overexpress dynaminK44A are completely blocked in
clathrin-dependent endocytosis. Using immunofluorescence
microscopy, the cells that express the mutant dynamin could be observed
individually for viral synthesis after infection at a low m.o.i. (<1)
(20). As pointed out above, HRV2 uncoating requires exposure to a low
pH environment for capsid modification and subsequent infection (14,
30). Therefore, it appeared important to first assess whether
overexpression of dynaminK44A has any influence on
endosomal pH.
Endosomal compartments were labeled by continuous internalization of
FITC- and Cy5-dextran into dynaminwt and
dynaminK44A cells. Cells were cooled, washed, and
immediately analyzed by flow cytometry. The pH values of all endosomal
compartments labeled under these conditions were then calculated from
the FITC/Cy5 ratio of the fluorescence intensities based on a
pH-standard curve (see Fig. 3A). Although fluid-phase uptake
of dextran was found to be reduced to ~70% in
dynaminK44A cells compared with dynaminwt
cells, the average pH of the labeled compartments was increased by 0.7 units in dynaminK44A cells (Fig.
5A). To investigate whether
endosomal and/or lysosomal acidification was affected, pulse-chase
experiments were carried out. Endosomes were pulse-labeled with FITC-
and Cy5-dextran by co-internalization for 5 min at 37 °C, followed
by a chase in dextran-free medium for the times indicated in Fig.
5B. The average intravesicular pH gradually decreased from
6.5 at 5 min to ~5.0 at 120 min in both cell types. However, that of
dynaminK44A cells was significantly higher at 15 and 55 min
of chase with respect to the values measured in dynaminwt
cells. This suggests that the pH in early endosomes and lysosomes is
unaffected, whereas acidification of late endosomes is altered most
severely. This finding precluded experiments involving the measurement
of de novo viral synthesis since the elevated pH in late
endosomes of mutant cells was expected to inhibit the conformational change required for uncoating. Consequently, internalization of HRV2
into dynaminwt and dynaminK44A cells was
analyzed first.
Internalization of HRV2 into Dynaminwt and
DynaminK44A Cells--
Lack of transferrin internalization
into cells overexpressing dynaminK44A (but not into control
cells) is shown in Fig. 6A.
Whereas dynaminwt cells internalized transferrin
irrespective of overexpression of dynaminwt, cells
overexpressing dynaminK44A were clearly negative for
transferrin staining. Binding and uptake of HRV2 were then followed by
indirect immunofluorescence using mAb 8F5 and Alexa 488-labeled
anti-mouse antibody. Exogenous dynamin expression was monitored via
rhodamine-labeled anti-HA antibody. First, binding of HRV2 to the cells
was monitored. Incubation was for 1 h at 4 °C, whereupon the
cells were washed, and cell surface-bound virus was visualized (Fig.
6B). Under these conditions, a typical plasma membrane
staining (small punctate and homogenous distribution over the entire
cell surface) was observed. Next, HRV2 internalization was
investigated. Since we found that virus could not be efficiently
removed from the cell surface (see above and Table I), HRV2 remaining
at the plasma membrane was masked with rabbit antiserum prior to
incubation with mAb 8F5. Control experiments confirmed that virus bound
to the plasma membrane could be rendered inaccessible to mAb 8F5 using
this procedure (data not shown). Dynaminwt and
dynaminK44A cells were incubated with HRV2 for 20 min at
34 °C and cooled; plasma membrane-bound virus was masked; and cells
were fixed, permeabilized, and processed for immunofluorescence. As
shown in Fig. 6C, cells expressing dynaminwt
exhibited a HRV2 staining pattern characteristic for early and late
endosomes that was clearly different from surface binding (compare with
Fig. 6B). In agreement with the experiments using K+ depletion, HRV2 was found to be internalized also into
cells expressing dynaminK44A as verified by HA staining. At
the level of resolution of fluorescence microscopy, internalization
appeared to be slightly less in the cells overexpressing the mutant
dynamin (arrows) with respect to cells not expressing the
exogenous protein.
Further confirmation for intracellular localization of HRV2 was
obtained by confocal fluorescence microscopy. An example of a
peripheral and a central layer of 12-layer stacks through a HA-dynaminwt cell and a HA-dynaminK44A cell is
shown in Fig. 7. In these images, HRV2 is
clearly seen within peripheral and perinuclear endosomes regardless of
whether dynaminwt or dynaminK44A was expressed.
Nevertheless, upon examination of 145 individual dynaminK44A-expressing mutant cells, only 68% were found
to internalize HRV2 (data not shown).
HRV2 Can Enter via LRP and/or VLDLR by a Clathrin-independent
Pathway--
HeLa cells express LDLR, VLDLR, and LRP, which all bind
HRV2. Nevertheless, it is currently unknown which of these receptors is
preferentially used for infection. RAP binds with high affinity to
VLDLR (Kd = 0.7 nM) and LRP
(Kd = 4 nM), but with much lower
affinity to LDLR (Kd = 500 nM) (42) and
has therefore been used extensively in competition studies. HRV2
binding and infection of familial hypercholesterolemia fibroblasts, which are devoid of LDLR, can be blocked by GST-RAP (6). Therefore, we
determined whether GST-RAP is able to inhibit viral uptake in
dynaminK44A-overexpressing cells. Dynaminwt and
dynaminK44A cells were preincubated with GST-RAP at 4 °C
for 60 min to block plasma membrane receptors. HRV2 was then added, and
incubation was continued at 34 °C in the presence of GST-RAP for 20 min. Cells were washed and prepared for immunofluorescence. In several independent experiments, as exemplified in Fig. 6D, a marked
reduction in HRV2 internalization by GST-RAP was seen regardless of
dynaminwt or dynaminK44A overexpression. This
specific effect of GST-RAP on HRV2 internalization strongly suggests
that virus is internalized preferentially by LRP and/or VLDLR in HeLa
cells expressing wild-type as well as mutant dynamin-1. The low numbers
of LDLR molecules present as a result of growing the cells in 10%
fetal calf serum containing enough cholesterol to down-regulate
receptor expression thus do not appear to appreciably contribute to
HRV2 internalization.
De Novo Viral Synthesis Occurs Regardless of Overexpression of
Dynaminwt or DynaminK44A--
To confirm viral
internalization and uncoating by means of viral replication in
dynaminK44A cells, the synthesis of viral protein was
monitored by indirect immunofluorescence after infection at a low
m.o.i. Cells induced to overexpress either dynaminwt of
dynaminK44A were infected with HRV2 at a m.o.i. of 1. The
cells were then incubated for 17 h at 34 °C and fixed, and
virus was visualized with mAb 8F5 followed by Alexa 488-labeled
anti-mouse antibody. As shown in Fig. 8,
cells stained positive for viral proteins irrespective of
overexpression of the mutant dynamin.
Exposure of cells to hypotonic medium followed by incubation in
the absence of extracellular potassium results in dissociation of
clathrin coats from the plasma membrane (43). As a consequence, internalization is impaired for the receptors for low density lipoprotein, epidermal growth factor, transferrin, and other membrane proteins carrying cytoplasmic amino acid sequences that interact with
the clathrin adapter complex AP2 (10, 18, 44).
Minor group rhinoviruses use members of the LDLR family, all possessing
internalization signals, for cell entry. The currently accepted
assumption that these receptors are exclusively internalized by the
clathrin-coated pit pathway clearly conflicts with the observed data,
an increase in the cytopathic effect after HRV2 infection of
potassium-depleted cells (18). To clarify this apparent contradiction,
we investigated the effects of K+ depletion on individual
steps in viral infection. Having previously characterized the infection
pathways of HRV2 and HRV14 in HeLa cells, these cells were chosen for
analysis. In these cells, potassium depletion inhibited uptake of
transferrin and RNA release from the virion is triggered by the low pH prevailing in
endosomal carrier vesicles and in late endosomes (13). It is intimately
linked with a structural change in the viral capsid that can be
detected with mAb 2G2, specific for C-antigenic subviral particles
(30). Comparison of potassium-depleted and control cells with respect
to the low pH-induced generation of C-antigen revealed that this step
was reduced to ~11% in the treated cells compared with the control
cells. This was found to be due to an increase in the endosomal pH by
~0.4 units (Fig. 3). The observed pH increase after K+
depletion is at variance with the findings of Madshus et al. (47), who reported that hypotonic shock leads to a temporary vesicular
alkalinization, with the acidic pH being restored after return to
isotonic medium. However, these workers internalized FITC-dextran for
6 h preceding the hypotonic shock and consequently recorded
preferentially the pH changes taking place in lysosomes.
It was further established that viral protein synthesis after infection
proceeds at an only slightly reduced level after potassium depletion
(Fig. 4). Therefore, one has to assume that transfer of the viral RNA
into the cytoplasm occurs with higher efficiency in potassium-depleted
cells and thereby compensates for the lower efficiency of structural
modification (generation of C-antigen) due to elevated pH. How this is
brought about is currently unknown, but might be related to changes in
the endosomal membrane potential facilitating translocation of the RNA.
Hypotonic shock/K+ depletion therefore not only dissociates
clathrin coats from the plasma membrane, but has multiple additional
effects that need to be considered when interpreting data from
experiments involving this treatment (47).
To avoid side effects of K+ depletion, internalization
experiments were also performed with HeLa cells overexpressing either dynaminwt or the nonfunctional mutant
dynaminK44A. With these cells, DeTulleo and Kirchhausen
(20) demonstrated the requirement of clathrin function for infection
with the major group virus HRV14. By double staining immunofluorescence
microscopy, these workers demonstrated that cells expressing the mutant
dynamin (as detected via a HA tag) also synthesized viral protein (as detected via a virus-specific antiserum). HRV14 uncoating and subsequent de novo synthesis of viral protein can occur at
neutral pH (8, 48). Uncoating of HRV2, however, is strictly dependent on pH To distinguish between LRP-dependent and -independent
internalization of the urokinase-type plasminogen activator receptor as
a function of polarized or unpolarized growth of Madin-Darby canine
kidney cells, competition with RAP was used (51). Evidence for
LRP/VLDLR being capable of clathrin-independent internalization of HRV2
was thus also substantiated by competition with GST-RAP. In the
presence of GST-RAP, the virus did not enter the cells, clearly
indicating that interaction with LRP or VLDLR was involved in the
process (Fig. 6D). Finally, clathrin-independent
internalization and uncoating were confirmed by demonstrating de
novo viral protein synthesis in the mutant cells (Fig. 8).
Comparison with Fig. 6 reveals that the pattern of viral fluorescence
was different. Whereas internalized virus appeared as perinuclear
punctate staining, viral protein originating from viral replication was
distributed evenly over the cytoplasm.
Carpentier et al. (52) demonstrated a reduction in
internalization of the specific LRP ligand,
Besides clathrin-dependent endocytosis, there are
alternative access ways to the cell. For example, caveolae have been
recently implicated in the uptake of various viruses (56-58); but
caveolin expression is very low in HeLa cells, and caveolae can be
excluded as entry vehicles (51, 59). Viral entry must thus occur via non-coated vesicles by a mechanism that is largely obscure.
Contrary to the belief that
glycosylphosphatidylinositol-anchored membrane proteins are
taken up in caveolae, clathrin- and caveolin-independent endocytosis of
a glycosylphosphatidylinositol-linked diphtheria toxin receptor in HeLa
cells was demonstrated (59). Apparently, a pathway involving non-coated
vesicles can be utilized by glycosylphosphatidylinositol-anchored proteins as well as by otherwise clathrin-dependent receptors, at least by LRP and VLDLR when their normal mode of entry is abolished by K+ depletion or expression of dynaminK44A.
Although our preliminary
results2 indicate that RAP
can be taken up as well in a clathrin-independent manner, it might be
considered that endocytosis of the multivalent virus could be triggered
in some way by multiple attachment of the receptors around the virion,
thereby inducing receptor clustering.
5.6 was strongly reduced due to an
elevation of the pH in endosomal compartments. However, K+
depletion only slightly affected de novo viral protein
synthesis, suggesting that productivity of viral RNA in the cytoplasm
is enhanced and thus compensates for the reduction in C-antigenic particles. The distinct steps in the entry pathway of HRV2 are thus
differently influenced by potassium depletion. Viral internalization under conditions of inhibited clathrin-dependent
endocytosis without the need to disturb the ionic milieu was confirmed
in HeLa cells overexpressing the nonfunctional dynamin-1 mutant K44A.
Unexpectedly, overexpression of dynamin-1 K44A resulted in elevated
endosomal pH compared with overexpression of wild-type dynamin.
INTRODUCTION
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
5.6) prevailing in
endocytic carrier vesicles and late endosomes (13, 14). These
modifications account for the formation of subviral "A-particles" sedimenting at 135 S that still contain the genomic viral RNA. Release
of the RNA from the protein shell creates subviral "B-particles" that sediment at 80 S. Both particles are detected early in infection and are probably intermediates (A-particles) and remainders
(B-particles) of the uncoating process (15). Whereas the native virus
is "D-antigenic," the subviral particles expose other epitopes and
are "C-antigenic" (16). We have shown that inhibition of endosomal
acidification by the specific vacuolar ATPase inhibitor bafilomycin
A1 completely blocks the conformational modification from
D- to C-antigenicity and prevents viral infection by HRV2 (14). As
demonstrated by size-selective release of fluid-phase markers, which
were co-internalized with the virus into endocytic compartments, pores
are opened in response to the low pH in the presence of the virus (17).
This suggests that the viral RNA enters the cytosol through a pore in
the endosomal membrane. The acid-induced conformational change thus
either precedes or is directly coupled to the transfer of the RNA
through the endosomal membrane into the cytosol. Modified subviral
particles are in part digested in lysosomes and in part recycled to the
cell surface and can therefore be found in the cell supernatant early
after infection. Upon arrival of the genomic RNA in the cytosol,
synthesis of new virus is initiated.
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--
All chemicals were obtained from Sigma unless
specified otherwise. Bafilomycin A1, kindly provided by Dr.
K. H. Altendorf (University of Osnabrück, Osnabrück,
Germany), was dissolved in Me2SO at 20 mM and
stored at
20 °C. The final concentration of Me2SO
(which was also added to the control samples) was kept below 1%.
GST-RAP was prepared as described elsewhere (22). Conjugation of
transferrin with fluorescein was carried out as described (23).
FITC-dextran (70 kDa) was extensively dialyzed against Tris-buffered
saline (pH 7.4) and finally against phosphate-buffered saline (PBS)
before use. Cy5.18-OSu (Cy5) was obtained from Amersham Pharmacia
Biotech (Buckinghamshire, United Kingdom) and coupled to dextran (70 kDa) as described (24). Alexa 488-labeled goat anti-mouse
antibody was purchased from Molecular Probes, Inc. (Eugene, OR);
rhodamine-labeled mouse anti-hemagglutinin (HA) monoclonal antibody was
obtained from Roche Molecular Biochemicals. Texas Red-coniugated
transferrin was dissolved in PBS (5 mg/ml) and stored at
20 °C.
Moviol 4-88 was purchased from Calbiochem and used at 10% in
water. [35S]Cysteine/methionine
(Tran35S-label) and Na125I were obtained from
American Radiolabeled Chemicals (St. Louis, MO)
125I-Labeled
-VLDL (1.6 × 108 cpm/mg)
was prepared by the IODO-GEN method (25) using Na125I as
described elsewhere (26). Lyophilized Staphylococcus aureus cells (IgG-sorb) were obtained from the Enzyme Center (Malden, NM).
Leibovitz L-15 medium was purchased from Life Technologies, Inc.
-VLDL Internalization--
Control or K+-depleted
HeLa cells were incubated in the respective
K+-containing or K+-free buffers containing 10 µg/ml 125I-labeled
-VLDL for 20 min at 37 °C. Cells
were cooled to 4 °C and washed with PBS2+. Plasma
membrane-bound
-VLDL was removed by incubation in the same buffers
containing 10 mg/ml suramin for 2 × 30 min at 4 °C. Cells were
lysed in lysis buffer; nuclei were removed by centrifugation at
10,000 × g for 1 min; and radioactivity in the
supernatant was determined by liquid scintillation counting.
Nonspecific uptake of 125I-labeled
-VLDL was determined
by incubation in the presence 10 mg/ml suramin.
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EXPERIMENTAL PROCEDURES
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DISCUSSION
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-VLDL, and FITC-Dextran into HeLa Cells Is Strongly Reduced upon
Hypotonic Shock and K+ Depletion--
Using the procedure
leading to dissociation of clathrin coats from the plasma membrane of
HEp-2 cells previously employed by Madshus et al. (18),
internalization of HRV2 into HeLa cells was studied. Transferrin is
generally accepted as a ligand, exclusively internalized via the
clathrin-coated pit pathway (34, 35). Therefore, plasma membrane
binding (at 4 °C for 1 h), as well as subsequent
internalization (after warming to 37 °C), was determined with
FITC-transferrin in control buffer and after K+ depletion.
Although binding was slightly augmented (110% of the control value),
internalization was inhibited to ~40% of the control value by
K+ depletion (Fig.
1A).
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Fig. 1.
Hypotonic shock and K+ depletion
inhibit internalization of FITC-transferrin (A),
FITC-dextran (70 kDa) (B), and
125I-labeled -VLDL
(C) into HeLa cells. Confluent cells in 12-well
plates were incubated with serum-free Leibovitz L-15 medium at 37 °C
for 30 min, followed by exposure to hypotonic Leibovitz L-15 medium
(diluted 1:1 with water) for 5 min and isotonic KCl-free buffer for 30 min. Control incubations were in isotonic Leibovitz L-15 medium and
KCl-containing buffer. In the case of FITC-transferrin, binding (50 µg/ml at 4 °C) and subsequent internalization (after warming to
37 °C for 20 min) were determined. Internalization was halted by
cooling the cells to 4 °C, and plasma membrane-bound
FITC-transferrin was removed by washing with acetic acid followed by
PBS2+ (pH 7.4). 125I-Labeled
-VLDL (1 µg/ml) and FITC-dextran (5 mg/ml) were continuously internalized in
the respective buffers for 20 min at 37 °C.
-VLDL was removed by
incubation with suramin (10 mg/ml) at 4 °C for 1 h (60).
Cell-associated fluorescence or radioactivity was measured; background
fluorescence and nonspecific binding of 125I-
-VLDL, as
determined by incubating the cells in the presence of 10 mg/ml suramin,
were subtracted. The means ± S.D. from three independent
experiments, each carried out in triplicate, are shown.
-VLDL is preferentially internalized via LDLR if not enriched
in apoE (36) and has also been shown to be internalized via
clathrin-coated pits (10). Cell monolayers were incubated with
125I-labeled rabbit
-VLDL for 20 min at 37 °C to
ensure localization in endosomes, but not in lysosomes (8, 14, 30);
plasma membrane-bound ligand was removed; and internalized ligand was determined by
-counting. As expected, hypotonic shock and
K+ depletion reduced internalization into HeLa cells to
~30% of the control value (Fig. 1C).
Efficiency of various treatments to detach plasma membrane-bound HRV2
from the cell surface
5.6 within late endocytic compartments and can thus be correlated
with viral internalization. As C-antigenic particles of HRV2 are also
recycled into the medium, they were determined both in the cell lysate
and in the supernatant. 35S-Labeled HRV2 was internalized
into control and K+-depleted HeLa cells for 20 min at
34 °C. Cells were pelleted and washed with cold EDTA/PBS, and
C-antigenic particles and native virus were then determined in cell
lysates by sequential immunoprecipitation. C-antigenic virus was
precipitated with mAb 2G2 (30), and the remaining native virus was
subsequently recovered with rabbit anti-HRV2 antiserum. From the
supernatant, only C-antigen was precipitated. In accordance with the
results described above, total radioactivity, as represented by the sum
of all immunoprecipitations (with mAb 2G2 and anti-HRV2 antiserum),
revealed that total cell-associated HRV2 plus C-antigenic particles
recycled into the supernatant were increased by 26% in
K+-depleted cells compared with control cells. However,
whereas under control conditions 74% of the total virus was present in the form of C-antigenic particles, only 8% of the total virus was
C-antigenic under K+-depleted conditions (Fig.
2). Since the conformational change reflects viral internalization into endosomes with pH
5.6, the substantial reduction in C-antigenic virus seen under
K+-depleted conditions suggests that internalized virus did
not encounter a pH low enough to allow for structural modification.
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Fig. 2.
Conformational change in native HRV2 to
C-antigenic subviral particles is decreased in K+-depleted
HeLa cells. 35S-Labeled HRV2 was internalized for 20 min into control and K+-depleted cells grown in 12-well
plates. Cells were washed with EDTA/PBS, and C-antigenic subviral
particles were immunoprecipitated with mAb 2G2 in cell lysates and in
the incubation medium. Native virus was then precipitated from the cell
lysates with rabbit anti-HRV2 antiserum. Radioactivity in the
precipitates was measured by liquid scintillation counting. Values
indicated are the means ± S.D. from three experiments, each
carried out in quadruplicate.
5.6, which is
necessary for the conformational modification (39). The difference of 0.4 pH units might thus easily explain the reduction in C-antigenic particles recovered from K+-depleted cells compared with
control cells (13).
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Fig. 3.
The average pH of endocytic compartments is
elevated in K+-depleted HeLa cells. A,
shown are the pH-standard curves of FITC/Cy5-dextran-labeled endosomes.
FITC-dextran (4 mg/ml; 70 kDa) and Cy5-dextran (1 mg/ml) were
internalized for 20 min at 37 °C, and the cells were incubated with
buffers of the given pH values in the presence of NaN3 and
ammonium acetate to allow for equilibration of intravesicular pH with
that of the incubation buffers. The mean values of FITC and Cy5
fluorescence of each sample were determined and used to calculate the
FITC/Cy5 ratio. B, labeled dextrans were internalized for 20 min at 37 °C in K+-depleted and control cells. Cells
were quickly cooled, washed, and analyzed by flow cytometry. The ratio
of the fluorescence intensities of FITC and Cy5 were calculated, and
the mean pH of labeled endocytic compartments was determined using the
pH-standard curve. The means ± S.E. from three individual
experiments with eight parallel determinations are shown.
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Fig. 4.
Viral protein synthesis is only slightly
reduced upon infection of K+-depleted cells. Control
or K+-depleted cells were challenged with HRV2 at a m.o.i.
of 100 for 20 min at 34 °C. Cells were transferred to the respective
bafilomycin A1-containing buffers and incubated for 40 min.
Thereafter, buffers were replaced with methionine-free medium
containing 2% FCS and bafilomycin A1. At 7 h
post-infection, [35S]methionine was added, and de
novo synthesized viral proteins were determined at 17 h
post-infection by immunoprecipitation and SDS-polyacrylamide gel
electrophoresis followed by fluorography. One representative
fluorograph out of five individual experiments carried out in duplicate
is shown. The experimental setup is indicated at the top.
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Fig. 5.
The endosomal pH is increased in HeLa cells
overexpressing dynaminK44A. Dynaminwt
(WT) and dynaminK44A (K44A) cells
were incubated with FITC-dextran and Cy5-dextran in DMEM for 20 min at
37 °C (A) or for 5 min at 37 °C (pulse), followed by a
chase by further incubation in DMEM without dextran (B).
Cells were cooled, washed with PBS, and analyzed by flow cytometry.
Endosomal pH was calculated based on a pH calibration curve (for
details, see "Experimental Procedures" and the legend to Fig.
3A). The means ± S.E. from three individual
experiments, each comprising eight determinations, are shown.
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Fig. 6.
HRV2 is internalized into HeLa cells
overexpressing dynaminwt or dynaminK44A.
A, cells overexpressing dynaminK44A do not
internalize transferrin. Upon induction of overexpression of
dynaminwt and dynaminK44A, cells were depleted
of endogenous transferrin and incubated with FITC-transferrin (50 µg/ml) for 20 min at 37 °C. Cells expressing exogenous HA-dynamin
were detected with rhodamine-labeled anti-HA antibody. B,
upon induction of overexpression of exogenous dynamin, cells were
incubated with HRV2 for 1 h at 4 °C, washed, and stained by
indirect immunofluorescence using mAb 8F5 and Alexa 488-labeled
anti-mouse antibody. C, cells were incubated with HRV2 for
20 min at 34 °C. Following blockage of plasma membrane-bound virus,
internalized virus was detected by indirect immunofluorescence as
described for B. Cells expressing HA-dynamins
(arrows) were stained with rhodamine-labeled anti-HA
antibody. D, dynaminwt and
dynaminK44A HeLa cells were preincubated with GST-RAP (100 µg/ml) at 4 °C for 1 h. HRV2 was added at a m.o.i. of 100, and incubation was continued for 20 min at 34 °C. Cells were cooled,
washed, and processed for immunofluorescence. Cells expressing
HA-dynamin are indicated by arrows.
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Fig. 7.
Confocal images of internalized HRV2.
Dynaminwt and dynaminK44A HeLa cells were
infected with HRV2 and processed for confocal immunofluorescence
microscopy as described in the legend to Fig. 6. Images of 12 layers
through the cell were recorded. Layer 11 (closer to the cell surface)
and layer 6 (cut through the cell body) are shown. Upper
panels, HRV2; lower panels, expression of the HA-tagged
dynamins (as described in the legend to Fig. 6).
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Fig. 8.
De novo viral synthesis occurs
after infection of dynaminK44A cells. Cells
overexpressing dynaminwt (upper panels)
and dynaminK44A (lower panels) were infected
with HRV2 at a m.o.i. of 1. Seventeen h post-infection, cells were
fixed, and HRV2 was stained with mAb 8F5 followed by Alexa 488-labeled
anti-mouse antibody. HA-dynamin expression was detected with
rhodamine-labeled anti-HA antibody. Cells were viewed in a fluorescence
microscope.
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-VLDL, a potent LDLR ligand (Fig. 1). Under the same
conditions, we found that HRV2 binding was increased, whereas
internalization appeared to be almost unimpaired. The former effect
might reflect a retention of receptors on the cell surface, as shown
for canine parvovirus (45), or an increased affinity, as reported
previously for LDLR (46); the latter finding, however, indicates that
the virus and, consequently, its receptor(s) are indeed capable of cell entry in a clathrin-independent manner. Possible candidates mediating viral internalization under these conditions are LRP and VLDLR, as LDLR
is clearly clathrin-dependent (10) (see below). When LDLR
expression is down-regulated by cholesterol, there is still internalization of HRV2, possibly by other members of the LDLR family
(6).
5.6, as demonstrated by complete inhibition of viral
replication by monensin (30) or bafilomycin A1 (14). Our
finding of an increase in the mean endocytic pH in potassium-depleted
cells thus called for caution and led us to determine the endosomal pH
in HeLa cells upon induction of dynaminK44A overexpression.
Indeed, a substantial increase in the pH was found in late endosomal
compartments, but not in lysosomes (Fig. 5). The latter finding is in
accordance with recent reports on lysosomal degradation of proteins
being unaffected in dynaminK44A-overexpressing cells (49,
50). Recently, association of dynamin-2 with late endosomes has been
reported; this results in defective trafficking from late endosomes to
the trans-Golgi network (50). As the membrane composition
affects the endosomal pH, it is likely that a defect in membrane
transport indeed leads to alterations in endosomal pH. We thus chose
not to use viral replication as an indirect measure for viral uptake,
but rather to determine internalized virus directly by
immunofluorescence microscopy. In accordance with the results of
K+ depletion, virus was found to be internalized into cells
overexpressing dynaminK44A, as shown by staining with
anti-HA tag antibody (Figs. 6 and 7).
2-macroglobulin conjugated to gold particles, into
3T3-L1 cells upon potassium depletion. Similarly, Sandvig et
al. (53) showed that uptake of Pseudomonas exotoxin,
another ligand of LRP, is severely reduced after potassium depletion in
L cells and baby hamster kidney cells. These findings strongly favor
internalization of these ligands by a clathrin-dependent pathway. This was recently confirmed by experiments demonstrating that
the deletion of internalization signals in the cytoplasmic tail of LRP
results in impaired internalization (54). On the other hand, it was
shown that the toxicity of Pseudomonas exotoxin is
enhanced under potassium depletion (55). Similar to the situation with
HRV2, multiple effects of K+ depletion complicate the
interpretation of these results. However, when intoxication is
increased in the latter case, Pseudomonas exotoxin must at
least be able to be internalized under conditions of K+
depletion. Therefore, LRP and/or VLDLR appears to be capable of being
taken up into the cell in the absence of functional clathrin coats to
some extent. Our results show that this occurs with substantial efficiency when HRV2 is bound.
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ACKNOWLEDGEMENTS |
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We particularly thank Dr. S. L. Schmid for HeLa cells expressing wild-type and mutant dynamins, Drs. A. Dautry- Varsat and C. Lamaze for instructions on the handling of the mutant cells, and Dr. K. H. Altendorf for the kind gift of bafilomycin A1.
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FOOTNOTES |
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* This work was supported by Austrian Science Foundation Grants P-12967-GEN and P-10618-MED (to R. F.) and P-12269-MOB (to D. B.) and Jubiläumsfonds der Österreichischen Nationalbank Grant 7511 (to R. F.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of
Pathophysiology, University of Vienna, Währinger Gürtel
18-20, A-1090 Vienna, Austria. Tel.: 43-1-40-400-5127; Fax:
43-1-40-400-5130; E-mail: renate.fuchs@akh-wien.ac.at.
Published, JBC Papers in Press, November 9, 2000, DOI 10.1074/jbc.M004722200
2 N. Bayer, D. Schober, M. Hüttinger, D. Blaas, and R. Fuchs, manuscript in preparation.
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ABBREVIATIONS |
---|
The abbreviations used are:
ICAM-1, intercellular adhesion molecule-1;
LDLR, low density lipoprotein
receptor;
LRP, low density lipoprotein receptor-related protein;
VLDLR, very low density lipoprotein receptor;
-VLDL,
-very low density
lipoprotein;
HRV2, human rhinovirus serotype 2;
HRV14, human rhinovirus
serotype 14;
GST, glutathione S-transferase;
RAP, receptor-associated protein;
FITC, fluorescein isothiocyanate;
PBS, phosphate-buffered saline;
HA, hemagglutinin;
DMEM, Dulbecco's
modified Eagle's medium;
RIPA, radioimmune precipitation assay;
MEM, minimal essential medium;
FCS, fetal calf serum;
dynaminK44A, dynamin-1 mutant K44A;
dynaminwt, wild-type dynamin-1;
MES, 2-(N-morpholino)ethanesulfonic
acid;
mAb, monoclonal antibody;
m.o.i., multiplicity of
infection.
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