The Polycystic Kidney Disease 1 Gene Product Modulates Wnt Signaling*

Emily KimDagger §, Thierry Arnould, Lorenz K. Sellin, Thomas Benzing, Melinda J. Fanparallel , Wolfram Grüning, Sergei Y. Sokolparallel , Iain Drummond**, and Gerd WalzDagger Dagger

From the Dagger  Laboratory of Molecular and Developmental Neuroscience, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, the  Renal Division and the parallel  Department of Molecular Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, and the ** Renal Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114

    ABSTRACT
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Abstract
Introduction
References

Two distinct signaling pathways, involving Wnt signaling and polycystin, have been found to be critical for normal kidney development. Renal tubulogenesis requires the presence of certain Wnt proteins, whereas mutations in polycystin impede the terminal differentiation of renal tubular epithelial cells, causing the development of large cystic kidneys that characterize autosomal dominant polycystic kidney disease. Polycystin is an integral membrane protein, consisting of several extracellular motifs indicative of cell-cell and cell-matrix interactions, coupled through multiple transmembrane domains to a functionally active cytoplasmic domain. We report here that expression of the C-terminal cytoplasmic domain of polycystin stabilizes soluble endogenous beta -catenin and stimulates TCF-dependent gene transcription in human embryonic kidney cells. Microinjection of the polycystin C-terminal cytoplasmic domain induces dorsalization in zebrafish. Our findings suggest that polycystin has the capacity to modulate Wnt signaling during renal development.

    INTRODUCTION
Top
Abstract
Introduction
References

The kidney is widely used as a model system to study the intricacies of tissue induction underlying vertebrate organogenesis. Kidney development begins with the condensation of mesenchyme around the ureteric bud in response to a signal from the ureter. Through reciprocal interactions between the ureteric bud and the mesenchyme, the metanephric mesenchyme evolves into the tubular epithelium that, together with a glomerulus, constitutes the mature nephron of the mammalian kidney (reviewed in Ref. 1). Embryonic kidney induction is thought to require the presence of Wnts, a highly conserved family of developmentally important secreted signaling molecules involved in embryonic induction, generation of cell polarity, and the specification of cell fate (reviewed in Refs. 2 and 3). The proposed pathway for Wnt signaling involves the inhibition of glycogen synthase kinase (GSK)1-3beta and the consequent posttranslational stabilization of soluble beta -catenin, leading to its accumulation in the cytoplasm and nucleus (reviewed in Ref. 3). In the nucleus, beta -catenin interacts with members of the TCF/LEF family of transcription factors to regulate gene expression (4, 5). Certain Wnt family members appear to mediate renal morphogenesis. Wnt-1, a Wnt family member not expressed in the kidney, nevertheless induces metanephric mesenchyme to differentiate into glomerular and renal tubular epithelia (6), whereas contact with the metanephric mesenchyme maintains Wnt-11 expression at the tip of the ureteric bud (7). Mesenchymal expression of Wnt-4 is required for kidney tubulogenesis; Wnt-4 -/- mice fail to form pretubular cell aggregates, a requisite stage of early tubule formation (8). Thus, in the developing kidney, Wnts appear to be involved in the reciprocal interactions between the ureteric bud and surrounding mesenchyme that enable the latter to differentiate into epithelial tubule cells.

Polycystin, the gene product of PKD1, is an integral membrane protein with 11 putative transmembrane helixes, an N-terminal extracellular region that contains motifs characteristic of cell-cell and cell-matrix interactions, and a C-terminal cytoplasmic domain of 226 amino acids (9, 10). Mutations of polycystin account for the majority of patients with autosomal dominant polycystic kidney disease, a common hereditary disease (1:1,000) of slowly progressive epithelial cyst formation. Mice lacking polycystin die perinatally with massively enlarged cystic kidneys. Analysis of PKD1 -/- mice indicates that polycystin is required during renal development for the elongation and maturation of tubular structures (11). Further functional characterization of polycystin and the genetic manipulation of full-length polycystin has been hindered by its complexity. To gain insight into the function of polycystin, we have begun to characterize the signaling pathways that are activated by the C-terminal domain of polycystin, and have recently demonstrated that the C-terminal 226 amino acids of polycystin trigger activation of the transcription factor AP-1 (12). This study demonstrates that the C-terminal cytoplasmic domain of polycystin activates Wnt signaling.

    EXPERIMENTAL PROCEDURES

Reagents and Plasmids-- MG132 (ProScript), lithium acetate (Sigma), cycloheximide (Sigma), and human epidermal growth factor (EGF) (Clonetics) were used at concentrations as indicated. The C-terminal domains of polycystin were expressed as membrane-bound fusion proteins using CD16-CD7 domains or the leader sequence of CD5 fused to the CH2 and CH3 domain of human IgG1 followed by the transmembrane region of CD7 as described previously (12, 16). The AP-1 and Siamois promoter constructs have been recently described (12, 13). The His6-tagged c-Jun and hemagglutinin (HA)-tagged ubiquitin were kindly provided by D. Bohmann, the HA-tagged GSK-3beta by J.R. Woodgett, and the EGF receptor HER453 by A. Ullrich. The cDNAs encoding the CD16.7-polycystin fusions were cut with XbaI and inserted in frame into the Spe1 site of the pXT7 vector, a derivative of pGEM4Z (Promega) and pSP64T. The GST-Axin 495-590 fusion protein contains amino acids 495 to 590 of mouse Axin fused to glutathione S-transferase (GST) and was generated by polymerase chain reaction, utilizing a myc-tagged full-length mouse axin as a template (kindly provided by F. Costantini).

Western Blot Analysis-- HEK 293T cells seeded in 6-well plates were transiently transfected by the calcium phosphate method. After incubation for 24 h, cells were lysed in sample buffer, fractionated on SDS-PAGE, and electroblotted to polyvinylidene difluoride membrane (NEN Life Science Products). For the time course, cells were incubated with cycloheximide for the indicated durations after pretreatment with lithium acetate or MG132 for 3 h. Western blot analysis was performed with M2 anti-Flag monoclonal antibody or anti-beta -catenin followed by incubation with horseradish peroxidase-coupled sheep anti-mouse immunoglobulin (Dako). Immobilized antibodies were detected by chemiluminescence (Pierce).

Subcellular Fractionation-- HEK 293T cells seeded in 10-cm plates were transiently transfected with the indicated constructs. After incubation for 24 h, cells were homogenized in 0.5 ml of 250 mM sucrose, 10 mM HEPES (pH 7.4), 2 mM MgCl2, 1 mM EGTA, 0.5 mM EDTA, 2 mM Na3VO4, containing 44 µg/ml phenylmethylsulfonyl fluoride, and protease inhibitor mixture (Boehringer Mannheim). After two successive centrifugations at 1,000 × g for 10 min at 4 °C, the anucleated supernatant was centrifuged at 100,000 × g at 4 °C for 1 h. The supernatant (S100 soluble fraction) was removed and the pellet (P100 membrane fraction) was resuspended in 0.1 ml of solubilization buffer (homogenization buffer containing 1% Triton X-100 and 1% SDS). After determining protein concentrations by the Bio-Rad DC protein assay, subcellular fractions containing equal amounts of protein were concentrated by acetone precipitation, eluted by heating in SDS-PAGE sample buffer, and subjected to SDS-PAGE and immunoblot analysis using an anti-beta -catenin monoclonal antibody (Santa Cruz Biotechnology, Inc.).

Ubiquitination Assay-- HEK 293T cells seeded in 10-cm plates were transiently transfected with the indicated constructs. After incubation for 24 h, cells were incubated with MG132 or medium alone for 3 h, lysed in 6 M guanidinium-HCl, 0.1 M Na2HPO4/NaH2PO4 (pH 8.0), 5 mM imidazole, and sonicated for 1 min. After centrifugation for 15 min at 4 °C, the His6-tagged c-Jun conjugates were precipitated from the cleared lysate with Ni2+-NTA-agarose (Qiagen) for 2 h at room temperature. Complexes were washed with 8 M urea, 0.1 M Na2HPO4/NaH2PO4 (pH 6.3), 0.01 M Tris, pH 8.0, and resuspended in sample buffer. Proteins were fractionated on SDS-PAGE, electroblotted to polyvinylidene difluoride membrane, and immunostained with rabbit polyclonal anti-HA serum (Santa Cruz) or rabbit polyclonal anti-Jun/AP-1 serum (Santa Cruz), followed by incubation with horseradish peroxidase-coupled anti-rabbit immunoglobulin (Amersham Pharmacia Biotech). Immobilized antibodies were detected by chemiluminescence (Pierce).

In Vitro Binding Assay-- [35S]Methionine-labeled GSK-3beta was generated in a 40-µl reaction mix, using the Promega TNT system following the instructions of the manufacturer. 10-µl of the reaction mix was then incubated with 2 µg of GST-PKD 115-226 or GST-Axin 495-590, and immobilized on glutathione-Sepharose in the presence of 450 µl reaction buffer (50 mM potassium phosphate, pH 7.5, 150 mM KCl, 1 mM MgCl2, 10% (v/v) glycerol, 1% Triton X-100, and protease inhibitors). The reaction mix was incubated for 2 h, washed three times in reaction buffer, and separated on a 10% SDS acrylamide gel. Radiolabeled GSK-3beta was detected by autoradiography. The amount of bound GSK-3beta was compared with 5% of labeled input protein equivalent to 0.5 µl of the labeling mix.

In Vivo Coimmunoprecipitation-- HEK 293T cells were transiently transfected with 5 µg of plasmid encoding HA-tagged GSK-3beta and 5 µg of plasmids encoding sIg.7, sIg.7-PKD 115-226 (16), or myc-Axin (kindly provided by F. Costantini) by the calcium phosphate method. After incubation for 24 h, cells were washed twice with phosphate-buffered saline and then lysed in 1 ml of 1% Triton X-100, 0.5% Nonidet P-40 buffer containing 150 mM KCl, 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, and protease inhibitors. Cell lysates containing equal amounts of total protein were incubated for 4 h at 4 °C with 30 µl of protein G-Sepharose beads (Amersham Pharmacia Biotech); the myc-Axin containing lysate was additionally incubated with 5 µg of anti-myc mouse monoclonal antibody (Calbiochem). The beads were washed extensively with lysis buffer, and bound proteins were fractionated by 10% SDS-PAGE. Western blot analysis was performed with anti-HA rabbit polyclonal antibody (Santa Cruz) followed by incubation with horseradish peroxidase-coupled donkey anti-rabbit immunoglobulin (Amersham Pharmacia Biotech). Immobilized antibodies were detected by chemiluminescence (Pierce).

GSK-3 Kinase Assay-- HEK 293T cells were transfected with plasmids encoding CD16.7-PKD 115-226 or control CD16.7 as indicated. Total DNA amount was titrated to 10 µg with control vector. Cells from one 10-cm dish were lysed 24 h after transfection in 1 ml of cold lysis buffer containing 1% Triton X-100, 150 mM NaCl, 10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, and a protease inhibitor mixture (Boehringer Mannheim). After centrifugation for 15 min at 4 °C, GSK-3beta was immunoprecipitated from the cleared lysate with 5 µg of monoclonal anti-GSK-3beta antibody (Transduction Laboratories) for 2 h at 4 °C. Immune complexes were immobilized by adding 40 µl of Gamma-Bind-Sepharose (Amersham Pharmacia Biotech) and washed twice with 800 µl of lysis buffer. During the second wash, 200 µl of resuspended beads were removed for Western blot analysis. Immunoprecipitates were washed with 500 µl of kinase reaction buffer (20 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 5 mM dithiothreitol) and resuspended in 50 µl of kinase reaction buffer containing 50 µM of cAMP-responsive element binding phosphopeptide (NEB). The assay was carried out in the presence of 20 µM unlabeled ATP and 10 µCi [gamma -32P]ATP for 30 min at 30 °C. A 25-µl aliquot was applied to a phosphocellulose membrane spin column (Pierce), washed with 500 µl of 75 µM phosphoric acid, and assayed by scintillation spectrophotometry. GSK-3beta immunoprecipitates, removed before the kinase assay, were analyzed by SDS-PAGE and Western blot, using the anti-GSK-3beta monoclonal antibody in combination with a goat anti-mouse horseradish peroxidase antibody (Dako) and enhanced chemiluminescence (Pierce).

Luciferase Assay-- HEK 293T cells seeded in 12-well plates were transiently transfected with a luciferase reporter construct, a beta -galactosidase expression vector (kindly provided by C. Cepko), and vectors directing the expression of CD16.7 fusion proteins, Wnt signaling components, and the EGF receptor HER453 as indicated. Total DNA amount was 3 µg/well. Cells were serum starved for 24 h, harvested in cold phosphate-buffered saline, and lysed in 100 µl of reporter lysis buffer (Promega) for 15 min at room temperature. Lysates were centrifuged at 14,000 rpm for 3 min to remove insoluble material. Luciferase activity was determined using a commercial assay system (Promega) following the manufacturer's instructions, and normalized for beta -galactosidase activity to correct for the transfection efficiency. EGF at 20 ng/ml was added for 8 h before the assay.

Embryo Microinjection-- Zebrafish embryos were injected at the 1-2 cell stage with 10-20 pg of either DNA expression vectors or capped mRNA. For DNA expression vector studies, embryos were injected with CD16.7-PKD 115-226 or the control vector CD16.7 in 200 mM KCl and 0.1% phenol red as a tracer. Capped mRNA for injection was synthesized using a MessageMachine kit (Ambion) and T7 polymerase. RNA was injected in a solution of 0.1% phenol red. Embryos were examined during epiboly, 5-10 h post-fertilization (hpf), and at 24 h.

    RESULTS

Polycystin Stabilizes Soluble beta -Catenin-- Recent studies indicate that Wnt signal transduction involves the inhibition of GSK-3beta activity, leading to a posttranslational stabilization of soluble beta -catenin that is commonly used as an indicator for Wnt signaling. We found that the C-terminal cytoplasmic domain of polycystin significantly elevated total beta -catenin steady-state protein levels (Fig. 1a) in HEK 293T cells that were transiently cotransfected with a Flag-tagged beta -catenin plasmid together with cDNA encoding the C-terminal cytoplasmic tail of polycystin, CD16.7-PKD 1-226. The C-terminal domain of polycystin was fused to the extracellular domain of CD16 and the transmembrane domain of CD7, forming a heterologous integral membrane protein. This approach has been used to delineate effector functions and protein-protein interactions of various receptor cytoplasmic domains by targeting them to the plasma membrane (14-16). The accumulation of beta -catenin was further localized to the C-terminal 113 amino acids of polycystin, whereas two controls, CD16.7, a construct with a stop codon shortly after the transmembrane domain and CD16.7-PKD 1-92, a construct containing the N-terminal 92 amino acids of the C-terminal cytoplasmic domain of polycystin, had no detectable effect on beta -catenin levels. As a positive control, exposure to lithium, a known inhibitor of GSK-3beta activity (17, 18), stabilized beta -catenin in CD16.7-transfected HEK 293T cells. Cells were cotransfected with a constant amount of plasmid DNA encoding Flag-tagged green fluorescent protein (F.gfp) to control for loading and transfection efficiency.


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Fig. 1.   The C-terminal domain of polycystin increases beta -catenin stability and inhibits c-Jun ubiquitination. a, the C-terminal domain of polycystin increases beta -catenin levels. Cell lysates of HEK 293T cells transiently cotransfected with Flag-tagged beta -catenin, F.gfp, and CD16.7 or CD16.7-PKD 1-92 (controls), CD16.7-PKD 1-226, or CD16.7-PKD 115-226 were immunoblotted with an anti-Flag antibody. b, beta -catenin is stabilized by MG132, lithium, and polycystin. HEK 293T cells were transiently transfected with control plasmids CD16.7 or CD16.7-PKD 115-226 and incubated with 40 µg/ml cycloheximide for 0, 1, 2, 4, 6, and 8 h after pretreatment with 40 µM MG132, 20 mM lithium acetate, or medium alone for 3 h as indicated. Cell lysates were immunoblotted with an anti-beta -catenin antibody. In all experiments, coexpression of F.gfp was utilized to monitor transfection efficiency and equal loading. F.gfp was stable over the course of 8 h, and not affected by lithium, MG132, or polycystin. A representative Western blot analysis of F.gfp expression is shown at the bottom of the panel. c, the C-terminal domain of polycystin increases soluble but not membrane beta -catenin levels. HEK 293T cells transiently transfected with CD16.7, CD16.7-PKD 1-226, or CD16.7-PKD 115-226 were lysed, anucleated, and fractionated at 100,000 × g. Lysates of subcellular fractions were immunoblotted with an anti-beta -catenin antibody (top). Amounts of cytoplasmic beta -catenin (black bars) were quantified by densitometric tracing after normalizing for membraneous fractions (white bars) (bottom). d, c-Jun ubiquitination is reduced in the presence of C-terminal polycystin. Cell extracts of HEK 293T cells transiently cotransfected with CD16.7 control, CD16.7-PKD 115-226, His6-tagged c-Jun, HA-tagged ubiquitin constructs as indicated and incubated with 40 µM MG132 or medium alone for 3 h. His6-tagged c-Jun conjugates were purified by nickel-chelate affinity chromatography and immunoblotted with an anti-HA antibody to detect c-Jun-ubiquitin conjugates. The lower panel shows the same blot developed with a polyclonal antibody against c-Jun.

To demonstrate that the accumulation of beta -catenin was mediated through the stabilization of beta -catenin, we examined the effect of polycystin on the half-life of endogenous beta -catenin while inhibiting protein synthesis. HEK 293T cells were transfected with a control plasmid CD16.7 or the construct CD16.7-PKD 115-226 and treated with the protein synthesis inhibitor cycloheximide (Fig. 1b). HEK 293T cells transfected with CD16.7 showed a gradual decline of beta -catenin protein levels over the course of 8 h. beta -catenin is subject to ubiquitin-dependent proteasome degradation, a process downstream of GSK-3beta that can be retarded by inhibiting ubiquitination or the proteasome (19). Preincubation of CD16.7-transfected cells with lithium, an inhibitor of GSK-3beta and ubiquitination, or MG132, a proteasome inhibitor, prolonged the half-life of beta -catenin. A similar prolongation of beta -catenin half-life was seen in HEK 293T cells transfected with CD16.7-PKD 115-226. To determine whether polycystin increased the cytoplasmic fraction of beta -catenin, transfected HEK 293T cells were fractionated and endogenous beta -catenin was visualized. An increase in cytoplasmic beta -catenin levels was observed in HEK 293T cells transfected with CD16.7-PKD 1-226 and 115-226, but not control transfected cells (Fig. 1c).

Polycystin Inhibits Ubiquitination of c-Jun-- The observed stabilization of beta -catenin by polycystin prompted us to examine whether polycystin inhibits the ubiquitination of other proteins regulated by GSK-3beta . The transcription factor c-Jun is regulated at the posttranslational level by ubiquitination and subsequent proteolysis (20). Both the stability and activity of c-Jun are regulated by mitogen-activated protein kinases and GSK-3beta (18, 21, 22). To investigate whether polycystin affects the ubiquitination of c-Jun in vivo, we coexpressed the C-terminal 112 amino acids of polycystin with histidine-tagged c-Jun and HA-tagged ubiquitin in HEK 293T cells. After purification of c-Jun under denaturing conditions, multi-ubiquitinated forms of c-Jun were detected by immunoblotting. We found that polycystin reduced the multi-ubiquitination of c-Jun, even in the presence of the proteasome inhibitor MG132 (Fig. 1d). This is consistent with a mechanism where polycystin suppresses the ubiquitination and subsequent degradation of c-Jun.

Polycystin Inhibits GSK-3beta Activity-- To further discriminate the mechanism through which the C terminus of polycystin stabilized beta -catenin, we examined the relationship of polycystin and GSK-3beta . Polycystin does not directly interact with GSK-3beta in vitro or in vivo. Bacterially expressed recombinant PKD 115-226 fused with GST did not bind GSK-3beta in vitro, although recombinant GST-Axin, a known binding partner, clearly interacted with GSK-3beta (Fig. 2a). Similarly, in vivo, a HA-tagged GSK-3beta coimmunoprecipitated with Axin, but not with the C terminus of polycystin in transfected HEK 293T cells (Fig. 2b). However, polycystin inhibited endogenous GSK-3beta in a cellular system, suggesting that polycystin requires an intermediary molecule to modulate GSK-3beta activity. In HEK 293T cells transfected with CD16.7-PKD 115-226, GSK-3beta kinase activity was decreased by 33-45% compared with cells transfected with the controls CD16.7 or CD16.7-PKD 1-92 (Fig. 2c). This inhibition of GSK activity is comparable with that reported for Wg in mouse fibroblasts which is sufficient to promote Wnt signaling (23), and to the inhibitory effect of insulin-like growth factor-1 or insulin in muscle cells (24).


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Fig. 2.   C-terminal polycystin inhibits GSK-3 activity in vivo, but does not associate with GSK-3beta in vitro or in vivo. a, Axin but not C-terminal polycystin binds GSK-3beta in vitro. The amount of [35S]methionine labeled GSK-3beta that bound to glutathione-Sepharose (GS), to the last 112 amino acids of polycystin fused to glutathione S-transferase (GST-PKD 115-226), or to GST-Axin was compared with 5% of labeled input protein. The GST fusion proteins were immobilized on glutathione-Sepharose and incubated with a mixture of equal amounts of 35S-GSK-3beta . b, Axin but not C-terminal polycystin binds GSK-3beta in vivo. The C terminus of polycystin was fused to sIg.7, a construct containing the leader sequence of human CD5 followed by the CH2 and CH3 domain of human IgG1, and the transmembrane region of human CD7. HEK 293T cells were transiently transfected with HA-tagged GSK-3beta and sIg.7 (control), sIg.7-PKD 115-226, or myc-Axin. Lysates were immunoprecipitated with protein G and blotted with anti-HA polyclonal antibody. The myc-Axin-containing lysate was additionally incubated with 5 µg of anti-myc mouse monoclonal antibody before immunoprecipitation with protein G. C, C-terminal polycystin inhibits GSK-3beta activity in vivo. HEK 293T cells, transfected with control CD16.7, CD16.7-PKD 1-92, or CD16.7-PKD 115-226 (left panel), were lysed and immunoprecipitated with an anti-GSK-3beta antibody, and assayed for kinase activity in two independent transfections. The amounts of precipitated endogenous GSK-3beta were demonstrated by Western blot analysis. HEK 293T cells, transfected with control CD16.7 and increasing amounts of CD16.7-PKD 115-226 (right panel), were lysed and immunoprecipitated with an anti-GSK-3beta antibody and assayed for kinase activity. Equal amounts of precipitated endogenous GSK-3beta were demonstrated by Western blot analysis.

Polycystin Induces TCF-dependent Gene Transcription-- To establish that the inhibition of GSK-3beta activity by polycystin functionally couples to downstream events of Wnt signaling, the formation of transcriptional active beta -catenin·TCF complexes was assayed by contransfecting a Xenopus Siamois luciferase reporter construct (-833pSiaLuc) into HEK 293T cells. The promoter of the dorsalizing homeobox gene Siamois is a direct target for the beta -catenin·TCF complex (25). Both CD16.7-PKD 1-226 and CD16.7-PKD 115-226, but not CD16.7-PKD 1-92, activated the Siamois promoter 10-12-fold (Fig. 3a). In contrast, CD16.7-PKD 1-226 and CD16.7-PKD 1-92, but not CD16.7-PKD 115-226 induced AP-1-dependent transcription (Fig. 3b). Wnt-dependent activation of the Siamois promoter requires the most proximal TCF-binding site (13). A truncated Siamois promoter construct (-245pSiaLuc) with a mutation of the proximal activating TCF-binding site showed significantly decreased responsiveness to polycystin (Fig. 4b) compared with the unmutated, truncated Siamois promoter (Fig. 4a), indicating that polycystin-mediated activation of the Siamois promoter depends upon the binding of beta -catenin·TCF complexes. The differences in signaling mediated between polycystin and EGF are demonstrated in Fig. 4c. EGF is a GSK-3 inhibitor that does not activate Wnt signaling. EGF stimulation of HEK 293T cells expressing EGF receptor did not activate the Siamois, but activated the AP-1 promoter 3-4-fold. In contrast, polycystin mediated a 10-12-fold induction of both AP-1 and Siamois promoter constructs. Thus, unlike growth factors such as EGF, which inhibit GSK-3beta without activating the Wnt signaling pathway, polycystin both inhibits GSK-3beta and activates Wnt signaling, a signaling pattern resembling that of canonical Wnt signaling molecules. Furthermore, polycystin augmented the activity of four Wnt signaling components, XWnt8, XDsh, rFz2, and beta -catenin (Fig. 5).


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Fig. 3.   Siamois and AP-1 are differentially activated by the C-terminal domain of polycystin. HEK 293T cells were cotransfected with the indicated constructs, a Siamois or AP-1 luciferase reporter construct, and a beta -galactosidase expression vector. Transactivation was determined after 24 h of incubation and expressed as relative light units (RLU) after normalization for beta -galactosidase activity. a, the Siamois promoter was activated by cotransfection with CD16.7-PKD 1-226 or CD16.7-PKD 115-226, but not CD16.7-PKD 1-92; b, in contrast, the AP-1 promoter was activated by CD16.7-PKD 1-226 or CD16.7-PKD 1-92 but not by CD16.7-PKD 115-226.


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Fig. 4.   Polycystin-mediated Siamois activation depends on the presence of a functional TCF binding site. a, the truncated Siamois promoter construct (-245pSiaLuc) containing only one activating TCF site was activated by CD16.7-PKD 1-226 or CD16.7-PKD 115-226 in HEK 293T cells. b, the polycystin-mediated responses were drastically reduced in the -245pSiaLuc construct containing a point mutation in the activating TCF site, demonstrating that transactivation of the Siamois promoter by polycystin depends critically on functionally active TCF binding sites. c, EGF stimulates the AP-1 promoter but fails to trigger the Siamois promoter. In this experiment, HEK 293T cells were cotransfected with the human EGF receptor and either the AP-1 or the Siamois promoter construct, followed by stimulation with EGF (20 ng/ml for 8 h). In contrast, polycystin (CD16.7-PKD 1-226) induces a greater than 10-fold activation of both promoter constructs. The depicted results are the composite of several experiments performed in triplicate, and represent mean ± S.D.


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Fig. 5.   Polycystin augments Wnt signaling. a, cotransfection with CD16.7-PKD 115-226 augmented the activation of the Siamois promoter by XWnt8, XDsh, rFz2, and beta -catenin in HEK 293T cells; b, the augmentation of Wnt signaling by polycystin was most pronounced for rFz2, resulting in a greater than 3.5-fold increase of promoter activity. All experiments were performed in triplicate; results represent mean ± S.D.

Polycystin Dorsalizes Zebrafish Embryos-- Previous work has shown that Wnt signaling is involved in specifying dorsal anterior fates and antagonizing signals specifying ventral posterior cell fates in the early embryonic axis (26-28). In zebrafish, beta -catenin induces an ectopic embryonic axis, whereas lithium produces hyperdorsalization (29, 30). To examine whether polycystin has a similar activity during early zebrafish development, embryos were injected at the 1-2 cell stage with either the control DNA expression vector CD16.7 or CD16.7-PKD 115-226. Control injected embryos showed no morphological abnormalities at any stage of development indicating that expression of the CD16.7 fusion protein does not disrupt normal development (Fig. 6a). In contrast, a marked dorsalizing effect of CD16.7-PKD 115-226 DNA was observed in injected embryos examined at 24 hpf (Fig. 6b). Embryos formed relatively normal head and brain tissue but invariably showed defects in posterior trunk and tail development. Injections of RNA encoding the same portion of the polycystin C terminus resulted in similarly dorsalized embryos (Fig. 6c). In the most extreme cases the embryo failed to complete epiboly resulting in a constriction around the yolk cell and an exposed yolk cell mass at 24 hpf (Fig. 6c). Trunk muscle and pronephric duct development was detectable although the form and organization of these tissues was disrupted (data not shown). In the least severely affected embryos, the tail formed, but defects in the development of ventral tissue just posterior to the yolk extension were always observed (Fig. 6d). The results indicate that when ectopically expressed in the context of early zebrafish development, the polycystin C terminus can have hyperdorsalizing effects, similar to that seen with GSK-3beta inhibition by lithium.


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Fig. 6.   Expression of C-terminal polycystin dorsalizes zebrafish embryos. Embryos were injected at the 1-2 cell stage. a, control vector CD16.7 injection has no effect on zebrafish development at 24 hpf; b, injection of CD16.7-PKD 115-226 severely disrupts trunk and tail development, whereas dorsal anterior structures are less affected. In two separate experiments, injection of CD16.7-PKD 115-226 RNA also resulted in a failure in posterior development. c and d, in a representative experiment, five of eight embryos did not develop posterior tissue (c) and two failed to complete epiboly resulting in embryos with exposed yolk at 24 hpf (arrows, c). The remaining three embryos showed similar but less severe defects in the development of ventral posterior tissue (d).


    DISCUSSION

Autosomal dominant polycystic kidney disease, the leading genetic cause of renal failure, is primarily caused by mutations in polycystin. Polycystin is required for normal tubulogenesis during renal development, but its precise physiologic function is not yet defined. Studies of PKD1-/- mutant mice indicate that early stages of tubule morphogenesis, such as nephrogenic condensation and epithelialization, remain intact (11). Expression of polycystin can be detected at E14 in the ureteric bud, and subsequently in the S- and Comma-shapes of the condensing mesenchyme (31-37). Cyst formation begins at embryonic day 15.5 (E15.5) in proximal tubules and progresses rapidly to replace the entire renal parenchyma. Wnt-4 is also required for renal tubulogenesis during this time; at E15.5 the mesenchyme of Wnt-4 -/- mice fails to aggregate and differentiate, lacking pretubular aggregates and more developed tubules (8). Kidneys of Wnt-4 -/- mice are growth retarded at E15.5 and agenic at E18.5, consisting of undifferentiated mesenchyme interspersed with branches of collecting duct epithelium. In the developing kidney, the expression pattern of polycystin appears to overlap with at least two known Wnt family members, Wnt-11, expressed in the ureteric bud (38), and Wnt-4, expressed in mesenchymal aggregates undergoing epithelial transition (8). Both spatially and temporally, Wnt and polycystin localization and signaling events coincide, supporting the hypothesis that polycystin may modulate Wnt signaling. Polycystin augmented the transcriptional activity of XWnt8, XDsh, rFz2, and beta -catenin, indicating that polycystin may serve to reinforce or maintain Wnt signaling during critical stages of renal tubulogenesis. The combinatorial interaction of Wnt signaling with other signaling pathways promotes functions that are different from the function of Wnt molecules alone (reviewed in Ref. 2). Although Wnt family members induce mesenchymal-epithelial conversion, cross-talk with polycystin may be required at a later stage to maintain tubular structure, polarity, and integrity. It is particularly interesting that beta -catenin, a transcriptional mediator of Wnt signaling, recently has been reported to regulate the reorganization of renal epithelial cell aggregates into long tubules (39).

Ectopic expression of many Wnt pathway components upstream of beta -catenin can be mimicked by the overexpression of beta -catenin in a variety of systems, suggesting that the role of certain Wnt family members is to increase levels of soluble beta -catenin (40). Genetic and biochemical studies indicate that secreted Wnts, through the family of frizzled receptors, activate disheveled and inhibit glycogen synthase kinase, to stabilize beta -catenin and facilitate transcription of certain target genes (reviewed in Refs. 2 and 3). In the absence of Wnt signaling, cytoplasmic beta -catenin is rapidly degraded in mammalian cells by a process involving the adenomatous polyposis coli tumor suppressor protein, ubiquitination, and proteasome (19, 41). Inhibition of the ubiquitination-proteasome pathway or overexpression of beta -catenin results in cytoplasmic accumulation of beta -catenin and its translocation into the nucleus (42). A modest inhibition of GSK-3beta activity appears sufficient to transduce Wnt signaling, raising the possibility that only a Wnt-sensitive subcellular GSK-3beta pool is able to regulate the stability of beta -catenin (reviewed in Ref. 3). Our data suggests that expression of the C-terminal domain of polycystin stabilizes soluble beta -catenin and increases the amount of transcriptionally active beta -catenin. Although GSK-3beta activity was reduced in the presence of polycystin, we were unable to detect a direct interaction of the C terminus of polycystin with GSK-3beta in vitro or in vivo, indicating that polycystin does not directly inhibit GSK-3beta kinase activity through a physical interaction.

A critical role for GSK-3beta in early vertebrate pattern formation has been clearly established in dorsoventral axis formation (43-45). Lithium, an inhibitor of GSK-3beta , dorsalizes zebrafish and Xenopus (30, 46), beta -catenin induces induction of a complete secondary body axis in both species (29, 40), and some upstream components of the Wnt-signaling pathway induce an ectopic embryonic axis in Xenopus (reviewed in Ref. 3). A marked dorsalizing effect of the terminal 112 amino acids of polycystin was observed in zebrafish embryos. The polycystin phenotype strikingly resembles the exaggerated dorsoanterior structures resulting from lithium treatment, indicating that polycystin may similarly inhibit GSK-3beta during zebrafish development (30).

The ligand of polycystin remains unidentified; hence, it is still unknown how extracellular events may influence the capacity of polycystin to modulate Wnt signaling. We speculate that ligand-receptor interactions governing the activity of the C-terminal portion of polycystin will be found to influence cytoplasmic-signaling cascades during critical stages of renal tubular development.

    FOOTNOTES

* This work was supported by a grant from the Polycystic Kidney Research Foundation (to G.W.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Supported by Public Health Service Grant MH-01147.

Dagger Dagger To whom correspondence should be addressed. Fax: 617-667-1610; E-mail: gwalz{at}bidmc.harvard.edu.

    ABBREVIATIONS

The abbreviations used are: GSK, glycogen synthase kinase; PKD, polycystic kidney disease; HEK, human embryonic kidney cells; PAGE, polyacrylamide gel electrophoresis; AP-1, activation protein-1; GST, glutathione S-transferase; F.gfp, Flag-tagged green fluorescent protein; EGF, epidermal growth factor; HA, hemagglutinin; hpf, hours post-fertilization.

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