Metalloprotease-Disintegrin MDC9: Intracellular Maturation and Catalytic Activity*

Monireh RoghaniDagger , J. David Becherer§, Marcia L. Moss§, Ruth E. Athertonparallel , Hediye Erdjument-Bromage**, Joaquin ArribasDagger Dagger , R. Kevin Blackburn§§, Gisela WeskampDagger , Paul Tempst**, and Carl P. BlobelDagger ¶¶

From the Dagger  Cellular Biochemistry and Biophysics Program, ** Molecular Biology Program, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center,  Cell Biology and Molecular Biology Program, Graduate School of the Cornell University Medical College, New York, New York 10021, the § Department of Molecular Biochemistry and the §§ Department of Analytical Chemistry, Glaxo Wellcome Research and Development Inc., Research Triangle Park, North Carolina 27709, and the Dagger Dagger  Laboratori de Recerca Oncologica, Hospital General, Psg. Vall d'Hebron, 08035 Barcelona, Spain

    ABSTRACT
Top
Abstract
Introduction
References

Metalloprotease disintegrins are a family of membrane-anchored glycoproteins that are known to function in fertilization, myoblast fusion, neurogenesis, and ectodomain shedding of tumor necrosis factor (TNF)-alpha . Here we report the analysis of the intracellular maturation and catalytic activity of the widely expressed metalloprotease disintegrin MDC9. Our results suggest that the pro-domain of MDC9 is removed by a furin-type pro-protein convertase in the secretory pathway before the protein emerges on the cell surface. The soluble metalloprotease domain of MDC9 cleaves the insulin B-chain, a generic protease substrate, providing the first evidence that MDC9 is catalytically active. Soluble MDC9 appears to have distinct specificities for cleaving candidate substrate peptides compared with the TNF-alpha convertase (TACE/ADAM17). The catalytic activity of MDC9 can be inhibited by hydroxamic acid-type metalloprotease inhibitors in the low nanomolar range, in one case with up to 50-fold selectivity for MDC9 versus TACE. Peptides mimicking the predicted cysteine-switch region of MDC9 or TACE inhibit both enzymes in the low micromolar range, providing experimental evidence for regulation of metalloprotease disintegrins via a cysteine-switch mechanism. Finally, MDC9 is shown to become phosphorylated when cells are treated with the phorbol ester phorbol 12-myristate 13-acetate, a known inducer of protein ectodomain shedding. This work implies that removal of the inhibitory pro-domain of MDC9 by a furin-type pro-protein convertase in the secretory pathway is a prerequisite for protease activity. After pro-domain removal, additional steps, such as protein kinase C-dependent phosphorylation, may be involved in regulating the catalytic activity of MDC9, which is likely to target different substrates than the related TNF-alpha -convertase.

    INTRODUCTION
Top
Abstract
Introduction
References

Metalloprotease-disintegrin proteins (also known as MDC1 proteins, metalloprotease/disintegrin/cysteine-rich proteins; ADAMs, a disintegrin and metalloprotease(1)) are a family of membrane-anchored glycoproteins that play a role in sperm-egg binding and fusion (2-10), muscle cell fusion (11), neurogenesis, and modulation of the Notch receptor signaling pathway in Drosophila melanogaster and in Caenorhabditis elegans (12-16) and processing of the pro-inflammatory cytokine TNF-alpha (17-19) (for recent reviews see Refs. 1 and 20). Metalloprotease disintegrins are usually comprised of several different protein modules as follows: an N-terminal signal sequence is followed by a pro-domain, metalloprotease domain, disintegrin domain, cysteine-rich domain, epidermal growth factor repeat, transmembrane domain, and cytoplasmic tail (see Fig. 2A). About half of the currently known metalloprotease disintegrins are predicted to be active metalloproteases due to a catalytic site consensus sequence (HEXXH) in their metalloprotease domain. The family members that lack a catalytic site are not predicted to be active metalloproteases. Both catalytically active and inactive metalloprotease disintegrins may play a role in cell-cell and cell-matrix interactions (1, 20).

It has been postulated that catalytically active metalloprotease disintegrins may have a role in the shedding or release of the ectodomain of membrane-anchored precursor proteins (17, 18, 20). Several different types of membrane proteins undergo protein ectodomain shedding (21), including cytokines such as TNF-alpha (17, 18) and the kit ligand (22), cytokine receptors like the TNF-alpha receptors (23, 24) and the p75 nerve growth factor receptor (25), adhesion proteins such as L-selectin (26, 27), and other proteins, including the beta -amyloid precursor protein (28, 29), the angiotensin-converting enzyme (30, 31), and the protein tyrosine phosphatases LAR and PTPsigma (32). Shedding of many of these protein ectodomains can be stimulated by phorbol esters such as PMA and can be inhibited by hydroxamic acid-based metalloprotease inhibitors (21).

In this study, we have evaluated the biosynthesis and in vitro catalytic activity of the widely expressed metalloprotease disintegrin MDC9 (33). Our results demonstrate that the pro-domain of MDC9 is removed in the secretory pathway, most likely by a furin-type pro-protein convertase, and that MDC9 is phosphorylated when cells are stimulated with PMA. Furthermore, this study provides the first evidence that the soluble metalloprotease domain of MDC9 is catalytically active, has a different peptide substrate specificity than the related TNF-alpha convertase, and that the catalytic activity of MDC9 can be inhibited by hydroxamic acid-based metalloprotease inhibitors. These results are discussed in the context of a potential role of MDC9 in protein ectodomain shedding.

    MATERIALS AND METHODS

Cell Culture-- COS-7 cells were grown in Dulbecco's modified Eagle's medium (10% fetal calf serum, 1% glutamine, 1% penicillin/streptomycin). Transient transfections of COS-7 cells were performed using LipofectAMINE (Life Technologies, Inc.) or via electroporation (Bio-Rad Genepulser II).

Western Blot Analysis-- Cells were lysed in TBS containing 1% Nonidet P-40 and protease inhibitors (4) on ice for 5 min, and centrifuged for 10 min in a Sorvall tabletop centrifuge. Cleared lysates were mixed with sample loading buffer, heated for 5 min at 95 °C in 50 mM dithiothreitol, separated by SDS-PAGE, and transferred to nitrocellulose. The nitrocellulose filters were blocked with 5% reconstituted dry milk, incubated with primary and secondary antibodies, and bound antibodies were visualized using the ECL chemiluminescence detection kit as described (34). Where indicated, the samples were deglycosylated with endoglycosidase H or PNGase F as described previously (35).

Cloning and Site-directed Mutagenesis-- The cDNA encoding the soluble MDC9 metalloprotease (consisting of nucleotides 1-1253 (33)) was synthesized as follows: a cDNA encoding for a Myc epitope tag (EQKLISEEDL) and an XbaI restriction site was added to the 3' end of the cDNA by polymerase chain reaction. The resulting polymerase chain reaction product was cleaved with EcoRI and XbaI and subcloned into a pcDNA3 vector (Invitrogen, San Diego, CA). The transformer site-directed mutagenesis kit (CLONTECH Laboratories Inc.) was used to introduce specific mutations, which were confirmed by DNA sequencing.

Cell-surface Biotinylation and Immunoprecipitation-- Transfected COS-7 cells were grown to confluency on 6-well plates, washed in PBS at 4 °C, and incubated with the non-membrane-permeable biotinylation reagent NHS-LC-biotin (Pierce) for 45 min on ice. After washing with 0.1 M glycine in PBS, the cells were lysed directly on the dish with cell lysis buffer (see above). Cleared cell lysates were subjected to immunoprecipitation as described (33). The immunoprecipitated material was separated by SDS-PAGE, transferred to nitrocellulose, probed with horseradish peroxidase-coupled streptavidin, and detected using the ECL chemiluminescence detection kit (Amersham Pharmacia Biotech).

Metabolic Labeling, Secretory Pathway Inhibitors, and Treatment with Recombinant Furin-- Transfected COS-7 cells were incubated in methionine/cysteine-free growth medium for 1 h and then pulse-labeled for 20 min with 200 µCi/ml Pro-Mix Label (NEN Life Science Products) (70% [35S]methionine and 30% [35S]cysteine). After the cells were washed in PBS, unlabeled growth medium was added for a chase period of up to 18 h. Where indicated, the secretory pathway inhibitors brefeldin A (5 µg/ml) and monensin (2, 10, and 25 µg/ml) were added during all steps of the experiment prior to lysis. For in vitro furin treatment, MDC9 precursor, immunoprecipitated from brefeldin A-treated cells, was incubated with 3 or 6 units of recombinant furin (kindly provided by Dr. R. Fuller) for 40 min at 37 °C in a modified furin cleavage buffer (35, 36). All immunoprecipitated materials were separated by SDS-PAGE. Following electrophoresis, the gels were fixed, incubated in Enhance solution (DuPont), dried, and exposed to Kodak XAR autoradiography film.

Purification of Soluble MDC9-MP-Myc and N-terminal Sequence Determination-- The soluble MDC9 metalloprotease was purified from supernatants of transiently transfected COS-7 cells using the anti-Myc-tag monoclonal antibody 9E10 coupled to protein G-Sepharose beads (37). The beads were washed with PBS, and the bound protein was eluted in 100 mM glycine, pH 3, neutralized immediately with M Tris/HCl, pH 8, and concentrated with a Centricon-10 concentrator (Amicon). The N terminus of the purified soluble MDC9 metalloprotease was determined as follows: after separation by SDS-PAGE, the sample was transferred to a polyvinylidene difluoride membrane (ProBlott, Applied Biosystems), stained with Coomassie Blue R-250, destained in 50% methanol, 10% acetic acid, and rinsed with doubly distilled H2O. The band of interest was excised, and the N-terminal amino acid residues were analyzed by automated Edman degradation, using an Applied Biosystems 477A sequenator, with instrument and procedure optimized for femtomole level analysis as described (38). The N-terminal sequence of the soluble MDC9 metalloprotease was identified as A206VLPQTR, which corresponds to the sequence immediately following the putative pro-protein convertase cleavage site RRRR205 in mouse MDC9 (33).

Insulin B-chain Cleavage Assay-- Purified soluble MDC9 MP-Myc, or recombinant purified TNF-alpha convertase (TACE) (18), or purified recombinant MMP-1 (39) were incubated with 50 µM oxidized insulin B-chain in 100 µl of reaction buffer (50 mM Tricine, pH 7.4, 200 mM NaCl, 10 mM CaCl2) at 37 °C for 1 or 6 h, in the presence or absence of 10 mM 1,10-phenanthroline. The reaction was stopped by addition of trifluoroacetic acid. The insulin B-chain and its cleavage products were detected by matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry using a Reflex III instrument (Bruker Franzen, Bremen, Germany) as described (40).

Inhibitor Titration of MDC9 to Determine Enzyme Concentration-- The MMP peptide substrate dinitrophenyl-proline cyclohexylalanineGC(Me)HK(N-methylanthranilic acid)NH2 (41), which is cleaved by MDC9 (see Table I), was diluted from a Me2SO stock to 30 µM in 10 mM Hepes, pH 7.5, containing 0.0015% Brij-35 (Buffer A). 60 µl of substrate was then added to 30 µl of CGS27023 (a potent hydroxamic acid-based inhibitor of MDC9, see below and Table III) at concentrations ranging from 0 to 10,000 nM. The reaction was initiated by addition of MDC9 (10 µl), so that the final dilution was 100-3000-fold and the reactions were run for 1-4 h at 22 °C. Initial velocities were calculated by measuring the fluorescence units of the reaction time and dividing by the fluorescence units when complete turnover of the substrate was observed (final reaction time). The initial velocities were plotted as a function of inhibitor concentration, and the curve was fit to Equation 1, a modified version of the Morrison equation (42). As an approximation, the Ki(app) was proposed to be comparable to the Ki as the substrate concentration was at least 5-fold below the Km. Note: an absolute number for the Km could not be determined as the substrate was not soluble at high concentrations.
  v=v<SUB>o</SUB><FENCE><FR><NU><RAD><RCD>(E<SUB>o</SUB>−I<SUB>o</SUB>−K<SUB>i(<UP>app</UP>)</SUB>)<SUP>2</SUP>+4K<SUB>i(<UP>app</UP>)</SUB>E<SUB>o</SUB></RCD></RAD>+(E<SUB>o</SUB>−I<SUB>o</SUB>−K<SUB>i(<UP>app</UP>)</SUB>)</NU><DE>2E<SUB>o</SUB></DE></FR></FENCE> (Eq. 1)
K<SUB>i(<UP>app</UP>)</SUB>=K<SUB>i</SUB>(1+<UP>S</UP>/K<SUB>m</SUB>) (Eq. 2)

Incubation of Candidate Substrate Peptides with MDC9 and Determination of Cleavage Sites and Kinetics-- Peptides corresponding to 12 amino acid residues surrounding the membrane proximal cleavage sites of selected proteins that are known to be released from the plasma membrane by metalloproteases (see Table I) were synthesized by Synpep (Dublin, CA). All peptides contained a dinitrophenyl (Dnp) group at their N terminus to facilitate detection at 350 nm. The peptide sequences and the reported cleavage sites are presented in Table I. MDC9 (1 nM) was incubated with each of the putative peptide substrates (30 µM) in 10 mM Hepes, pH 7.2, containing 0.0015% Brij (Sigma). Reactions were timed to allow approximately 5-20% turnover of the substrate. Reactions were quenched using 1% heptafluorobutyric acid, and products were separated by high pressure liquid reverse phase chromatography (C18 column, Vydac, Hisperia, CA) with absorbance monitored at 350 nm. Turnover was quantitated by integrating peak areas of the substrate and product(s). Liquid chromatography-mass spectrometry was used to determine the masses of the products and therefore the cleavage site(s) recognized by MDC9. Briefly, digestion mixtures were passed over a hypersil C18 column, and after UV detection at 350 nm, the sample was routed into the ionspray source of a Sciex API-III triple quadrupole mass spectrometer. Specificity constants were calculated from initial velocities using the equation: kcat/Km = (% turnover/100)/([E]·time). Conditions of kcat/Km were verified by running the reactions at more than one substrate concentration. The enzyme concentration for MDC9 was determined as described above. For the TNF and the MMP peptide substrates, two products were formed, and individual specificity constants were determined for each of the products since they were generated independently from one another (data not shown).

Evaluation of the Inhibitory Potential of Cysteine Switch Peptides-- Synpep Corp. (Dublin, CA) synthesized all of the Cys-switch peptides (see Table IV). All solvents and buffers used were sparged with argon to avoid oxidation of the thiol group. Peptides were dissolved in dimethyl formamide to yield a stock solution of 50 mM and were diluted as described below such that the final concentration of dimethyl formamide in the assay was 1%. MDC9 activity was measured using streptavidin-coated scintillation proximity assay (SPA) beads and a biotinylated peptide corresponding to the sequence of the cleavage site in pro-TNF-alpha . The digests were performed by the addition of 25 µl of titrated inhibitor in 3% dimethyl formamide (10 mM Hepes, pH 7.5) to 25 µl of substrate (biotin-SPLAQA*VRSSSRTP(3H) S-NH2) in Hepes with 0.1% bovine serum albumin. Reactions were initiated by addition of 25 µl of recombinant MDC9 or recombinant TACE (in Hepes with 0.1% bovine serum albumin). The digestion was quenched by addition of streptavidin SPA beads (Amersham Pharmacia Biotech) in EDTA. The final concentration of substrate in the assay was 200 nM. Enzyme was 1.0 nM, and cysteine-switch peptides were titrated from 100 µM with 3-fold dilution, 11 points per curve. Under the above conditions the substrate concentrations are significantly less than Km, and the Ki can be determined directly by plotting percent inhibition versus the log of the inhibitor concentration, where Ki(app) = Ki (1 + [S]/Km). No effects on the Ki were observed whether 0.5 mM cysteine was included or not.

Inhibition Constants Against Batimastat, Marimastat, CGS 27023, and TNF-alpha Protease Inhibitor-- Synthesis of batimistat, CGS 27023, and the TNF-alpha protease inhibitor has been described (18, 43). The substrate Dnp-PChaGC(Me)HK(NMA)NH2 (41) was diluted from a Me2SO stock solution to a concentration of 30 µM in buffer containing 10 mM Hepes, pH 7.5, and 0.0015% Brij-35 (Buffer A). A 60-µl aliquot was removed and added to a black Polyfiltronics 96-well plate. Inhibitor was diluted 1/10 from 10 mM Me2SO stock solutions. Three-fold serial dilutions in Me2SO were performed across a Costar 96-well round bottom plate. The inhibitor was diluted 30-fold into Buffer A. An aliquot of inhibitor, 30 µl, was then added to the black Polyfiltronics 96-well plate. MDC9 was diluted 60-300-fold into Buffer A, and 10 µl was added. Fluorescence was monitored at an excitation of 343 nm and emission of 450 nm. Reactions were run from 0.5 to 4 h at 22 °C and liquid chromatography/mass spectrometry analysis was performed to determine cleavage sites in the substrate.

In a similar manner, Ki values were determined for 19-kDa truncated collagenase (MMP-1), 20-kDa truncated collagenase 3 (MMP-13), stromelysin-1 (MMP-3), and 50-kDa truncated gelatinase B (MMP-9) using the same fluorogenic substrate. Assays were conducted in a total volume of 0.180 ml of assay buffer (200 mM NaCl, 50 mM Tris, 5 mM CaCl2, 10 µM ZnSO4, 0.005% Brij 35, pH 7.6) in each well of a black 96-well microtiter plate. 19-kDa collagenase-1, 20-kDa collagenase-3, stromelysin-1, and 50-kDa gelatinase B concentrations were adjusted to 500 pM, 30 pM, 5 nM, and 100 pM, respectively. A dose response was generated using an 11-point 3-fold serial dilution with initial starting concentrations of 100, 10, or 1 µM. Inhibitor and enzyme reactions were incubated for 30 min at room temperature and then initiated with 10 µM fluorogenic substrate. The product formation was measured after 45-180 min. Finally, Ki values for these inhibitors against TACE were determined using the same SPA assay as described earlier for the cysteine-switch peptides. In all cases, the conditions were such that the substrate concentrations were significantly less than Km, and the Ki can be determined directly by plotting percent inhibition versus the log of the inhibitor concentration, where Ki(app) Ki (1 + [S]/Km).

Phorbol Ester Treatment of CHO Cells-- A CHO cell line stably expressing MDC9 or a control CHO cell line stably transfected with a pcDNA3 vector alone was labeled either with 35S-Pro-Mix Label (NEN Life Science Products) (70% [35S]methionine and 30% [35S]cysteine) or incubated in the presence 1 mCi/ml of 32P for 3 h. Cells were then treated for 5 min in the presence or absence of 1 µM PMA. Immunoprecipitated material was separated by SDS-PAGE and detected by autoradiography as described above.

    RESULTS

Intracellular Maturation of MDC9-- To evaluate the biosynthesis and maturation of mouse MDC9 in the secretory pathway, pulse-chase experiments were performed using transiently transfected COS-7 cells expressing MDC9. Immunoprecipitations at different time points show that MDC9 is first synthesized as a precursor of 110 kDa (Fig. 1A) which is later processed to an 84-kDa form. Only the processed form of MDC9 with a molecular mass of 84 kDa is immunoprecipitated from cell-surface biotinylated cells, demonstrating that pro-domain removal most likely occurs intracellularly (Figs. 1C and 2B, and see Ref. 33). The subcellular localization of pro-domain removal was further defined by assessing the effect of the secretory pathway inhibitors brefeldin A and monensin on MDC9 processing. Brefeldin A, which blocks vesicle budding in the endoplasmic reticulum (44), completely blocked processing of MDC9 at 5 µg/ml. Monensin, which is thought to prevent transport past the medial-Golgi apparatus (45), partially blocked MDC9 processing at 2 and 10 µg/ml and completely blocked processing at 25 µg/ml (Fig. 1B). Western blot analysis of an endoglycosidase H-treated sample of MDC9-expressing COS-7 cells showed that both the precursor and mature forms of MDC9 were sensitive to endo H (Fig. 1C, lane 2). Treatment of an identical sample with PNGase, which removes most or all N-linked carbohydrate residues, resulted in a faster migrating mature form of MDC9 compared with the endo H-treated sample. In contrast, the MDC9 precursor appeared to comigrate in the endo H- and PNGase-treated samples. The cell surface-labeled processed form of MDC9 displayed a similar change in migration on SDS-PAGE after endo H and PNGase treatment compared with the processed form of MDC9 detected by Western blot. This result suggests that at least one, but not all, of the N-linked carbohydrate moieties of mature MDC9 acquires resistance to endo H treatment through the conversion of high mannose glycans into complex carbohydrates in the medial-Golgi network. Taken together, these results suggest that MDC9 is proteolytically processed in the secretory pathway after passage through the medial-Golgi apparatus.


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Fig. 1.   Pulse-chase analysis of MDC9 maturation in COS-7 cells and in vitro cleavage by recombinant furin. A, MDC9 was immunoprecipitated from transiently transfected COS-7 cells with antibodies against the MDC9 cytoplasmic tail at different time points. B, comparison of MDC9 immunoprecipitated from transfected COS-7 cells immediately after pulse labeling (lane 1) and after a 3-h chase (lane 2), with MDC9 immunoprecipitated after a 3-h chase in the presence of the secretory pathway inhibitors brefeldin A (5 µg/ml, lane 3) or monensin (2, 10, and 25 µg/ml, lanes 4-6). C, Western blot of (upper panel) and immunoprecipitation from cell-surface biotinylated COS-7 cells (lower panel)-expressing MDC9. Prior to electrophoresis, the samples were deglycosylated with endo H (lane 2), or with PNGase F (lane 3), or mock-incubated as control (lane 1). In both cases the primary antibody was against the MDC9 cytoplasmic tail. D, MDC9 immunoprecipitated after a pulse label and 3-h chase from untreated COS-7 cells expressing MDC9 (lane 1) was compared with the products resulting from treatment of the MDC9 precursor with 3 units/20 µl or 6 units/20 µl of recombinant furin (lanes 3 and 4, respectively). The MDC9 precursor was immunoprecipitated from cells incubated with brefeldin A during the 3-h pulse (lane 2).

Since MDC9 contains a consensus cleavage sequence for the pro-protein convertase furin between its pro- and metalloprotease domain, and because furin resides predominantly in the trans-Golgi network (45), furin or related pro-protein convertases are good candidates for processing MDC9 in the secretory pathway. To provide direct evidence for a potential role of furin in processing MDC9, we immunoprecipitated the precursor of full-length MDC9 from brefeldin A-treated COS-7 cells and incubated it with recombinant furin (36) (Fig. 1D). The furin-treated MDC9 precursor co-migrated with the MDC9 as it is processed in vivo in COS-7 cells. Increasing amounts of furin also generated an additional faster migrating band, perhaps due to an additional cleavage site that is not accessible to the membrane-anchored form of furin or a related pro-protein convertase in vivo. Alternatively, this site may be less efficiently cleaved and therefore only utilized when relatively high concentrations of furin are added in vitro.

To evaluate the sequence requirements for intracellular processing and metalloprotease activity of MDC9, the following mutations were introduced into both a soluble and a membrane-anchored form of MDC9 by site-directed mutagenesis (see Fig. 2A): 1) removal of the furin cleavage site (Delta 4R) between the pro- and metalloprotease domains; 2) conversion of the putative pro-domain cysteine-switch residue (46, 47), which is predicted to inhibit the protease during biosynthesis by binding to the active site, into an alanine (pro-Cys right-arrow Ala); 3) mutation of the "catalytic" glutamic acid in the metalloprotease active site (MP-Glu right-arrow Ala) (48).


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Fig. 2.   Processing of full-length and soluble MDC9 mutants. A, diagram of the domain organization of MDC9 and other metalloprotease disintegrins, and of the mutations generated for this study. B, Western blot (top panel), or immunoprecipitation of cell-surface biotinylated material (bottom panel) from COS-7 cells transfected with vector alone (lane 1), or expressing full-length wild-type MDC9 (lane 2), MDC9 lacking the predicted furin cleavage site (Delta 4R, lane 3), MDC9 with a catalytic site mutation (MP Glu right-arrow Ala, lane 4) and MDC9 with a pro-domain cysteine-switch mutation (pro-Cys right-arrow Ala, lane 5). Lane 6 shows a Western blot of a separate sample of wild-type full-length MDC9 expressed in COS-7 compared with the Delta 4R mutant (lane 7), which were separated on a 7.5% SDS-gel to better resolve subtle differences in their relative motility. A rabbit polyclonal antibody against the MDC9 cytoplasmic domain was used as primary antibody. C, Western blots of cell lysates (top panel) and of culture supernatants (bottom panel) of COS-7 cells transfected with the vector (lane 1), or expressing soluble wild-type MDC9 (lane 2), or soluble MDC9 Delta 4R (lane 3), pro-Cys right-arrow Ala (lane 4), or MP Glu right-arrow Ala (lane 5). The 9E10 monoclonal antibody against the Myc tag was used as primary antibody. Pro-MP refers to the soluble precursor protein, whereas MP refers to the processed metalloprotease domain lacking the pro-domain. The 51- and 80-kDa proteins that are also present in the nontransfected control lane most likely represent proteins with which the 9E10 monoclonal antibody against the Myc tag cross-reacts. D, material resulting from affinity purification of MDC9 was separated under non-reducing conditions by SDS-PAGE and stained with Coomassie Brilliant Blue. IgG indicates immunoglobulin that coelutes from an affinity column with the 9E10 monoclonal antibody. N-terminal sequence analysis of the soluble MDC9 metalloprotease (MP) confirmed the identity of the purified protein and demonstrated that pro-domain removal had occurred by cleavage next to the furin cleavage site (RRRR, see "Materials and Methods"). DTT, dithiothreitol; WT, wild type.

The effects of the mutations on intracellular processing were first tested by comparing the relative amounts of MDC9 precursor and processed forms in Western blots and in cell-surface biotinylated samples (Fig. 2B). On Western blots, the 110-kDa precursor of MDC9 and the processed form of 84 kDa can both be detected (Fig. 2B). However, only the processed form of 84 kDa can be immunoprecipitated from cell surface-labeled samples (Figs. 1C and 2B, see also Ref. 33). Both removal of the predicted furin cleavage site, and the Glu right-arrow Ala mutation in the catalytic site did not detectably affect MDC9 processing and appearance on the cell surface (Fig. 2B). However, removal of the putative cysteine-switch residue in the pro-domain led to a dramatic decrease of the processed 84-kDa form of MDC9 in the cell lysate as well as on the cell surface. The Delta 4R and the Pro-Cys right-arrow Ala mutations also resulted in small amounts of additional faster migrating bands compared with wild-type MDC9, which most likely result from aberrant processing.

Similar results were obtained with a recombinant soluble pro- and metalloprotease domain of MDC9 (Fig. 2C). The wild-type soluble protein and all three mutant forms were detected as proteins of ~47 kDa in Western blots of COS-7 cell lysates (Fig. 2A, predicted molecular mass of soluble wild-type MDC9 is 44.29 kDa). The supernatant of cells expressing the soluble wild-type and the MP Glu right-arrow Ala mutant contained mainly a ~28-kDa protein, suggesting that the pro-domain had been removed intracellularly (Fig. 2C, lower panel, lanes 2 and 5). N-terminal sequence analysis of the affinity purified soluble wild-type MDC9 metalloprotease domain (Fig. 2D, lane 1) confirmed that processing had occurred immediately after the predicted pro-protein convertase cleavage site in MDC9 (RRRR205-AVLPQTR, see "Materials and Methods"). Soluble MDC9 Delta 4R was secreted both as an unprocessed precursor of 47 kDa and as a processed form of ~33 kDa (Fig. 2C, lane 3). Evidently processing of MDC9 can occur in the absence of a furin cleavage site at an adjacent position. This finding prompted a re-evaluation of the processing of the full-length MDC9 Delta 4R mutant by comparing its migration to that of the wild-type MDC9 on a Western blot of proteins that had been separated on a 7.5% SDS-polyacrylamide gel. Under these conditions the full-length Delta 4R mutant migrated slightly slower than the wild-type protein (Fig. 2B, lanes 6 and 7), consistent with a cleavage at an adjacent but distinct site. Similar to the behavior of the full-length pro-Cys right-arrow Ala mutant, very little of the soluble pro-Cys right-arrow Ala MDC9 mutant could be detected in the supernatant, although the protein was clearly present in the cell lysate (Fig. 2C, lane 4).

Catalytic Activity of Soluble MDC9, TACE, and MMP-1 on the Insulin B-chain-- To determine whether MDC9 is catalytically active, the soluble wild-type metalloprotease was affinity purified from COS-7 cell supernatants (Fig. 2D). The affinity purified material was incubated with the insulin B-chain peptide, a generic protease substrate consisting of 30 amino acid residues (49). The resulting insulin B-chain fragments were identified by mass spectrometry (Fig. 3, A-C). After 1-h incubation with MDC9, cleavage between insulin B-chain residues Tyr16 and Leu17, and Tyr26 and Thr27 could be detected (Fig. 3A). After 6 h, the substrate had been completely converted into products resulting from cleavage between Tyr16 and Leu17, and Tyr26 and Thr27. No processing was seen after 6 h in the presence of the metalloprotease inhibitor 1,10-phenanthroline (Fig. 3A) or after co-incubation of the insulin B-chain for 6 h with a similar amount of the MP Glu right-arrow Ala catalytic site mutant purified under identical conditions (data not shown). When the insulin B-chain was incubated with purified soluble TACE (Fig. 3B) or MMP-1 (Fig. 3C) under identical conditions, in each case only one cleavage between Tyr16 and Leu17 was observed. As would be expected, 1,10-phenanthroline inhibited TACE- and MMP-1-mediated cleavage of the insulin B-chain (Fig. 3, B and C). Under the conditions used in this study, the apparent cleavage specificity of MDC9, TACE, and MMP-1 on the insulin B-chain is thus more restricted than what has been reported for most other proteases, including related snake venom metalloproteases (49, 50).


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Fig. 3.   MALDI-TOF analysis of insulin B-chain cleavage products produced by soluble MDC9. Matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) mass spectrometric traces of products generated from incubating the oxidized insulin B-chain with affinity purified soluble MDC9 for 1, 6, or 6 h in the presence of 1,10-phenanthroline (A); recombinant TACE (B); and MMP-1 for 6 h or 6 h in the presence of 1,10-phenanthroline (C). The numbers above the major peaks indicate the experimental monoisotopic masses (m/z). D, sequence of the insulin B-chain and the predicted protonated monoisotopic masses of the oxidized insulin B-chain and of the corresponding cleavage products. The predicted protonated monoisotopic masses were calculated with Procomp software, assuming that both cysteine residues in the insulin B-chain have the mass of the oxidized form of cysteine, cysteic acid (C(O3H)). The cleavage sites are marked by arrowheads.

Kinetic Analysis of Candidate Substrate Peptide Cleavage by MDC9-- Several different types of membrane proteins are known to be released from the plasma membrane by metalloproteases. To evaluate further the cleavage specificity of MDC9 and to test whether MDC9 might in principle have a role in protein ectodomain shedding, the soluble MDC9 metalloprotease was incubated with peptides corresponding to cleavage sites of specific membrane proteins that are known to be released from the membrane by metalloproteases (21). Table I shows that MDC9 was able to cut the cleavage site peptide of the beta -amyloid precursor protein (beta -APP) (28), tumor necrosis factor alpha  (TNF-alpha ) (51), the p75 TNF receptor (p75-TNFR) (52), and the c-kit ligand-1 (KL-1) (53). To determine the cleavage sites, the fragments of peptides that were processed by affinity purified MDC9 were analyzed by mass spectrometry. As indicated in Table I, MDC9 did not cleave any of the peptides tested here at the same site that is known to be used by sheddases in cells in vivo. The c-kit ligand and TNF-alpha peptide were cut in more than one position by recombinant MDC9. Furthermore, we found that affinity purified MDC9 was able to process an MMP substrate peptide that is not processed by TACE.2 Finally, affinity purified soluble MDC9 did not process cleavage site peptides for the interleukin-6 receptor (54), the p55 TNF receptor (p55-TNFR) (55), pro-transforming growth factor alpha  (TGF-alpha ) (56), and L-selectin (55) under the conditions used here.

                              
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Table I
Peptides mimicking the membrane-proximal cleavage site of proteins known to be shed by metalloproteases were synthesized and evaluated for cleavage by MDC9
An asterisk denotes the predicted cleavage site based on published studies (see "Results" for references). Those peptides that were resistant to MDC9 proteolysis are denoted by minus. For those peptides where multiple cleavages occurred, the products are listed in order of abundance with P1 > P2 > P3.

The cleavage kinetics of the beta -APP peptide, the TNF-alpha peptide, and also of the MMP peptide by MDC9 were determined as described under "Materials and Methods." The kinetic analysis revealed that the kcat/Km of beta -APP cleavage by MDC9 is 4.3-fold higher than the kcat/Km for cleavage of the P1 site in TNF-alpha , 6.8-fold higher than the kcat/Km for cleavage of the P2 site in TNF-alpha , and 21-fold higher than the kcat/Km for cleavage of the MMP substrate peptide by MDC9 (Table II).

                              
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Table II
kcat/Km values were determined, as described under "Materials and Methods," for three of the peptides listed in Table I
%cv indicates the coefficient of variation in percent.

Hydroxamic Acid Derivatives and Cysteine-switch Peptides as Inhibitors of MDC9 Metalloprotease Activity-- Since TACE can be inhibited by hydroxamic acid-based metalloprotease inhibitors, we asked whether (a) hydroxamic acid derivatives are also inhibitors of the MDC9 metalloprotease activity, and (b) whether there is some specificity of four previously described hydroxamic acid-based inhibitors toward MDC9 or TACE. Table III shows the Ki of different inhibitors toward TACE and MDC9, as well as toward the matrix-type metalloproteases MMP-1, MMP-3, MMP-9, and MMP-13. Under the in vitro cleavage conditions used here, CGS 27023 was the most selective for MDC9 over TACE (~50-fold), whereas marimastat was the most selective for TACE over MDC9 (~12-fold). These data further demonstrate that MDC9 has a unique inhibitor profile which does not resemble that of TACE or any of the MMPs tested here.

                              
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Table III
Inhibition constants were determined for each enzyme using the MMP fluorescent substrate assay
For TACE, which does not cleave the MMP fluorescent substrate, the TNF SPA peptide assay was used instead (see "Materials and Methods").

Metalloprotease disintegrins are predicted to be kept inactive during biosynthesis via a cysteine-switch mechanism (46, 47). The predicted cysteine-switch residue is defined as an odd-numbered cysteine that is only present in the pro-domain of metalloprotease disintegrins that contain a catalytic site but not in those that lack a catalytic site. To provide experimental support for this hypothesis, we examined the inhibitory potential of peptides mimicking the predicted cysteine-switch sequence of human or mouse MDC9, human TACE, human ADAM10 (KUZ/MADM), and mouse ADAM12 (meltrin alpha ) on the catalytic activity of mouse MDC9 and human TACE. As shown in Table IV, the TACE and mouse MDC9 cysteine-switch peptides were the best inhibitors of TACE and MDC9. The human MDC9 cysteine-switch peptide, which differs from the mouse peptide in one amino acid residue, inhibited both proteases somewhat less efficiently. The cysteine-switch peptides of ADAM10 and ADAM12 did not significantly inhibit TACE or MDC9 up to a concentration of 100 µM.

                              
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Table IV
IC50M) values for various Cys-switch peptides were determined against TACE and MDC9 using the SPA TNF peptide assay (see "Materials and Methods")
(>150) denotes those peptides for which an IC50 could not be determined but where the IC50 is higher than 150 µM since in all cases inhibition of the enzyme by these Cys peptides was less than 20% at 100 µM. h, human; m, mouse.

Phorbol Ester-dependent Phosphorylation of MDC9-- Metalloprotease-induced protein ectodomain shedding of a large variety of cell-surface proteins, including cytokines, cytokine receptors, adhesion proteins, and the beta -amyloid precursor protein, can be triggered by addition of the phorbol ester PMA to cells, presumably by activating protein kinase C (21, 57). A CHO cell line stably expressing MDC9 (see Fig. 4, lane 2) was labeled with 32P in the absence or presence of the phorbol ester PMA. MDC9 immunoprecipitated from these cells was only found to be labeled with 32P in extracts of PMA-treated cells (Fig. 4, lane 5) but not in unstimulated cells (Fig. 4, lane 4).


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Fig. 4.   Phorbol ester-dependent phosphorylation of MDC9. Immunoprecipitation of MDC9 from [35S]Met/Cys-labeled (lane 2) or from 32P-labeled CHO cells stably expressing MDC9, incubated in the presence (lane 5) or absence of PMA (lane 4). Control immunoprecipitations were performed on CHO stably transfected with the expression vector and labeled with 35S (lane 1) or 32P (lane 3).


    DISCUSSION

This study provides the first analysis of the intracellular maturation and catalytic activity of the metalloprotease disintegrin MDC9. Several lines of evidence demonstrate that MDC9, like MDC15 (35), is processed in a late compartment of the secretory pathway, most likely by a pro-protein convertase such as furin. First, processing of MDC9 can be prevented by addition of the secretory pathway inhibitors brefeldin A (44) and monensin (45), suggesting that MDC9 is processed after passage through the medial-Golgi apparatus. Second, incubation of pro-MDC9 with recombinant furin in vitro results in pro-domain removal. Third, N-terminal sequence analysis of a soluble secreted MDC9 metalloprotease domain confirmed that processing occurs next to the predicted furin cleavage site (RRRR) in cells. Finally, after deletion of the furin cleavage site, both full-length membrane-anchored MDC9 and a soluble pro- and metalloprotease-domain construct appear to be processed at a different site than the wild-type proteins. Nevertheless, removal of the furin site did not noticeably affect the efficiency of processing of full-length membrane-anchored MDC9. One explanation for this result could be that additional cleavages in the MDC9 pro-domain normally occur after processing at the furin site, something that has also been observed for mMDC15 (35). Such additional cleavage sites in the pro-domain may facilitate its removal after cleavage by furin and may only become apparent after the furin cleavage site is eliminated.

The first evidence for catalytic activity of MDC9 was obtained by incubating affinity purified soluble MDC9 with the insulin B-chain, a generic protease substrate. Our results demonstrate that MDC9, TACE, and MMP-1 can all cleave the insulin B-chain, albeit with somewhat different specificity. Cleavage of the insulin B-chain and of the general protease inhibitor alpha 2-macroglobulin (58) may thus be useful initial assays to assess the catalytic activity of other metalloprotease disintegrins.

Because metalloprotease disintegrins have been hypothesized to function in protein ectodomain shedding, we evaluated the ability of soluble MDC9 to cleave selected candidate substrate peptides that mimic the cleavage sites of proteins that are shed from the plasma membrane by a metalloprotease activity. Of the eight peptides tested here, the beta -APP peptide was the most efficiently cleaved, followed by the pro-TNF-alpha , p75-TNFR, and KL-1 peptides. MDC9 did not process peptides mimicking the cleavage site of the p55-TNFR, L-selectin, pro-TGF-alpha , and the interleukin-6 receptor. Mass spectrometric analysis of the products generated by MDC9 revealed that none of the four substrate peptides were cleaved at the site that is used during release of the corresponding protein from cells. Therefore MDC9 is most likely not the secretase responsible for processing the proteins that were included in the present study. Indeed, TACE can process TGF-alpha , L-selectin, and p55-TNFR peptides in the correct position in vitro.3 Furthermore, a targeted deletion of TACE results in the inability of cells to release TNF-alpha , TGF-alpha , and L-selectin from the cell surface, although it remains formally possible that some of these substrates are cleaved by another protease that needs TACE to become activated (17, 59).

With respect to beta -APP processing, TACE is apparently the major protein kinase C-dependent alpha -secretase in cultured cells, and it also cleaves the beta -APP peptide at the major alpha -secretase site in vitro (28, 60). Although MDC9 clearly does not target the major alpha  secretase site in vitro, in light of the relatively efficient processing of the beta -APP peptide observed here it will be interesting to determine whether MDC9 may have some role in beta -APP processing, perhaps restricted to certain cell types. In this context it is worth pointing out that MDC9 cleaves beta -APP within a peptide sequence (HHQK) which apparently plays a role in inducing neurotoxic microglia (61). Finally, we note that a standard MMP peptide substrate is cleaved in a similar fashion by MDC9 and MMPs -1, -3, -9, and -13, but not by TACE, and that MDC9 cleaves the TNF-alpha peptide at the same two sites as MMP-1 and MMP-9.4 It will thus also be interesting to evaluate whether MDC9 may have substrates in the extracellular matrix in vivo.

These results provide the first evidence for differences in the substrate specificity and cleavage site selection between two distinct metalloprotease disintegrins, at least as far as soluble peptide substrates and soluble proteases are concerned. In addition to any inherent substrate specificity of metalloprotease disintegrins in vitro, additional factors may determine which substrates are cleaved in vivo. These determinants may include potential targeting or recognition events between the substrate and other parts of the metalloprotease disintegrin protein, such as the disintegrin domain, cysteine-rich region, or epidermal growth factor repeat (20). Furthermore, access to substrates may also depend on regulated or constitutive co-localization in the same subcellular compartment(s) (35).

An evaluation of the inhibitory potential of different hydroxamic acid-based metalloprotease inhibitors, which are known to potently inhibit TACE (17, 18, 62), revealed that these compounds are also potent inhibitors of MDC9 and of certain matrix-type metalloproteases. Furthermore, we observed differences in the selectivity of the compounds tested here toward TACE or MDC9. An extension of this approach may therefore yield even more selective inhibitors of these or other metalloprotease disintegrins.

To address whether removal of the prodomain, for example by furin, is a prerequisite for catalytic activity (33, 35, 63, 64), we tested whether peptides corresponding to the cysteine-switch sequence of various metalloprotease disintegrins inhibit the catalytic activity. In matrix type metalloproteases (46, 47) and the snake venom metalloproteases adamalysin II (47), the free sulfhydryl of the cysteine-switch residue in the pro-domain is thought to bind to the Zn2+ ion in the catalytic site. Our results suggest that MDC9 and TACE are indeed both inhibited by their respective cysteine-switch peptides. The inhibitory potential of the mouse MDC9 cysteine-switch peptide for mouse MDC9 is similar to that of the adamalysin II cysteine-switch peptide for adamalysin II (47). The relatively potent inhibition of mouse MDC9 by its cysteine-switch peptide may explain why mutating the cysteine-switch residue strongly decreased transport of full-length and soluble MDC9 to the cell surface. In analogy to the protease subtilisin, where the pro-domain is thought to function as intramolecular chaperone (65), the interaction of the free sulfhydryl residue with the active site may be necessary for the pro- and metalloprotease domains of MDC9 to fold properly. If so, removing the cysteine-switch residue might prevent productive folding, resulting in retention of the mutant protein by endoplasmic reticulum-resident chaperones and subsequent degradation (66). In contrast to MDC9, mutating the cysteine-switch residue in soluble human ADAM12 (meltrin alpha ) did not affect its secretion (58), suggesting differences in the contribution of the cysteine-switch peptide to how these two proteins fold.

Finally, we demonstrate that MDC9 is phosphorylated after addition of PMA to cells. Since protein ectodomain shedding can be triggered with phorbol esters (21, 57), this result raises the intriguing possibility that the metalloprotease activity of MDC9 could be regulated by inside-out signaling due to protein kinase C-dependent phosphorylation of the cytoplasmic tail. However, since PMA stimulation can result in the phosphorylation of a variety of different proteins, the physiological significance of this result remains to be determined.

In conclusion, this study demonstrates that MDC9 is made as a precursor which is processed by a pro-protein convertase in the secretory pathway. Processing removes the presumably inhibitory pro-domain of MDC9 and is thus most likely a prerequisite for the protease to become active. After pro-domain removal, there may be additional mechanisms to regulate protease activity, such as phosphorylation of the cytoplasmic tail by protein kinase C. Furthermore, this study provides the first evidence that MDC9 is catalytically active, that it has a different substrate specificity than TACE, and that it can be inhibited by hydroxamic acid-based inhibitors. The catalytically active metalloprotease domain of MDC9 will be an important tool for further biochemical and functional analysis of this widely expressed metalloprotease disintegrin protein.

    ACKNOWLEDGEMENTS

We thank Dr. R. Fuller for kindly providing recombinant furin; Dr. J. E. Rothman for aliquots of the 9E10 anti-Myc tag monoclonal antibody; Dr. P. C. Andrews for providing the Procomp software; Dr. R. Andrews and C. Haffner at Glaxo Wellcome for the synthesis of the hydroxamate inhibitors used in this study; and L. Lum and J. Schlöndorff for comments on the manuscript.

    FOOTNOTES

* This work was supported in part by National Institutes of Health Grant R55GM51988, by a grant from Glaxo Wellcome (to C. P. B.), by National Science Foundation Grant DBI-9420123 (to P. T.), and by the Memorial Sloan-Kettering Cancer Center Support Grant NCI-P30-CA-08748.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

parallel Supported in part by the Ford Foundation.

¶¶ To whom correspondence should be addressed: Cellular Biochemistry and Biophysics Program, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, Box 368, 1275 York Ave., New York, NY 10021. Tel.: 212-639-2915; Fax: 212-717-3047; E-mail: c-blobel{at}ski.mskcc.org.

The abbreviations used are: MDC, metalloprotease/disintegrin/cysteine-rich proteins; ADAMs, a disintegrin and metalloprotease; TNF, tumor necrosis factor; TNFR, TNF receptor; PMA, phorbol 12-myristate 13-acetate; TACE, TNF-alpha convertase; beta -APP, beta -amyloid precursor protein; PAGE, polyacrylamide gel electrophoresis; PNGase F, peptide N-glcosidase; PBS, phosphate-buffered saline; TGF-alpha , transforming growth factor alpha ; MMP, matrix metalloproteinase; MP, metalloprotease; endo H, endoglycosidase H; Tricine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine; SPA, scintillation proximity assay; Dnp, dinitrophenyl; MALDI-TOF, matrix-assisted laser-desorption/ionization time-of-flight; CHO, Chinese hamster ovary.

2 M. Moss, submitted for publication.

3 R. Black, personal communication.

4 M. Moss, unpublished observation.

    REFERENCES
Top
Abstract
Introduction
References

  1. Wolfsberg, T. G., and White, J. M. (1996) Dev. Biol. 180, 389-401[CrossRef][Medline] [Order article via Infotrieve]
  2. Primakoff, P., Hyatt, H., and Tredick-Kline, J. (1987) J. Cell Biol. 104, 141-149[Abstract]
  3. Blobel, C. P., Wolfsberg, T. G., Turck, C. W., Myles, D. G., Primakoff, P., and White, J. M. (1992) Nature 356, 248-252[CrossRef][Medline] [Order article via Infotrieve]
  4. Blobel, C. P., Myles, D. G., Primakoff, P., and White, J. W. (1990) J. Cell Biol. 111, 69-78[Abstract]
  5. Myles, D. G., Kimmel, L. H., Blobel, C. P., White, J. M., and Primakoff, P. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 4195-4198[Abstract]
  6. Almeida, E. A. C., Huovila, A.-P. J., Sutherland, A. E., Stephens, L. E., Calarco, P. G., Shaw, L. M., Mercurio, A. M., Sonnenberg, A., Primakoff, P., Myles, D. G., and White, J. M. (1995) Cell 81, 1095-1104[Medline] [Order article via Infotrieve]
  7. Evans, J. P., Kopf, G. S., and Schultz, R. M. (1997) Dev. Biol. 187, 79-93[CrossRef][Medline] [Order article via Infotrieve]
  8. Evans, J. P., Schultz, R. M., and Kopf, G. S. (1997) Dev. Biol. 187, 94-106[CrossRef][Medline] [Order article via Infotrieve]
  9. Evans, J. P., Schultz, R. M., and Kopf, G. S. (1995) J. Cell Sci. 108, 3267-3278[Abstract/Free Full Text]
  10. Shilling, F. M., Krätzschmar, J., Cai, H., Weskamp, G., Gayko, U., Leibow, J., Myles, D. G., Nuccitelli, R., and Blobel, C. P. (1997) Dev. Biol. 186, 155-164[CrossRef][Medline] [Order article via Infotrieve]
  11. Yagami-Hiromasa, T., Sato, T., Kurisaki, T., Kamijo, K., Nabeshima, Y., and Fujisawa-Sehara, A. (1995) Nature 377, 652-656[CrossRef][Medline] [Order article via Infotrieve]
  12. Fambrough, D., Pan, D., Rubin, G. M., and Goodman, C. S. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 13233-13238[Abstract/Free Full Text]
  13. Rooke, J., Pan, D., Xu, T., and Rubin, G. M. (1996) Science 273, 1227-1230[Abstract]
  14. Pan, D., and Rubin, J. (1997) Cell 90, 271-280[Medline] [Order article via Infotrieve]
  15. Wen, C., Metzstein, M. M., and Greenwald, I. (1997) Development 124, 4759-4767[Abstract/Free Full Text]
  16. Sotillos, S., Roch, F., and Campuzano, S. (1997) Development 124, 4769-4779[Abstract/Free Full Text]
  17. Black, R., Rauch, C. T., Kozlosky, C. J., Peschon, J. J., Slack, J. L., Wolfson, M. F., Castner, B. J., Stocking, K. L., Reddy, P., Srinivasan, S., Nelson, N., Boiani, N., Schooley, K. A., Gerhart, M., Davis, R., Fitzner, J. N., Johnson, R. S., Paxton, R. J., March, C. J., and Cerretti, D. P. (1997) Nature 385, 729-733[CrossRef][Medline] [Order article via Infotrieve]
  18. Moss, M. L., Jin, S.-L. C., Milla, M. E., Burkhart, W., Cartner, H. L., Chen, W.-J., Clay, W. C., Didsbury, J. R., Hassler, D., Hoffman, C. R., Kost, T. A., Lambert, M. H., Lessnitzer, M. A., McCauley, P., McGeehan, G., Mitchell, J., Moyer, M., Pahel, G., Rocque, W., Overton, L. K., Schoenen, F., Seaton, T., Su, J.-L., Warner, J., Willard, D., and Becherer, J. D. (1997) Nature 385, 733-736[CrossRef][Medline] [Order article via Infotrieve]
  19. Rosendahl, M. S., Ko, S. C., Long, D. L., Brewer, M. T., Rosenzweig, B., Hedl, E., Anderson, L., Pyle, S. M., Moreland, J., Meyers, M. A., Kohno, T., Lyons, D., and Lichenstein, H. S. (1997) J. Biol. Chem. 272, 24588-24593[Abstract/Free Full Text]
  20. Blobel, C. P. (1997) Cell 90, 589-592[CrossRef][Medline] [Order article via Infotrieve]
  21. Hooper, N. M., Karran, E. H., and Turner, A. J. (1997) Biochem. J. 321, 265-279[Medline] [Order article via Infotrieve]
  22. Huang, E. J., Nocka, K. H., Buck, J., and Besmer, P. (1992) Mol. Biol. Cell 3, 349-362[Abstract]
  23. Porteu, F., and Nathan, C. (1990) J. Exp. Med. 172, 599-607[Abstract]
  24. Porteu, F., Brockhaus, M., Wallach, D., Engelmann, H., and Nathan, C. F. (1991) J. Biol. Chem. 266, 18846-18853[Abstract/Free Full Text]
  25. DiStefano, P. S., and Johnson, E. M., Jr. (1988) Proc. Natl. Acad. Sci. U. S. A. 85, 270-274[Abstract]
  26. Kishimoto, T. K., Jutila, M. A., Berg, E. L., and Butcher, E. C. (1989) Science 245, 1238-1241[Medline] [Order article via Infotrieve]
  27. Kahn, J., Walcheck, B., Migaki, G. I., Jutila, M. A., and Kishimoto, T. K. (1998) Cell 92, 809-818[Medline] [Order article via Infotrieve]
  28. Selkoe, D. J. (1996) J. Biol. Chem. 271, 18295-18298[Free Full Text]
  29. Sisodia, S. S. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 6075-6079[Abstract]
  30. Oppong, S. Y., and Hooper, N. M. (1993) Biochem. J. 292, 597-603[Medline] [Order article via Infotrieve]
  31. Ramchandran, R., and Sen, I. (1995) Biochemistry 34, 12645-12652[Medline] [Order article via Infotrieve]
  32. Aicher, B., Lerch, M. M., Muller, T., Schilling, J., and Ullrich, A. (1997) J. Cell Biol. 138, 681-696[Free Full Text]
  33. Weskamp, G., Krätzschmar, J. R., Reid, M., and Blobel, C. P. (1996) J. Cell Biol. 132, 717-726[Abstract]
  34. Inoue, D., Reid, M., Lum, L., Krätzschmar, J., Weskamp, G., Myung, Y. M., Baron, R., and Blobel, C. P. (1998) J. Biol. Chem. 273, 4180-4187[Abstract/Free Full Text]
  35. Lum, L., Reid, M. S., and Blobel, C. P. (1998) J. Biol. Chem. 273, 26236-26247[Abstract/Free Full Text]
  36. Bravo, D. A., Gleason, J. B., Sanchez, R. I., Roth, R. A., and Fuller, R. S. (1994) J. Biol. Chem. 269, 25830-25837[Abstract/Free Full Text]
  37. Harlow, E., and Lane, D. (1988) Antibodies: A Laboratory Manual, pp. 511-522, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
  38. Tempst, P., Geromanos, S., Elicone, C., and Erdjument-Bromage, H. (1994) Methods Comp. Methods Enzymol. 6, 248-261[CrossRef]
  39. Becherer, J. D., Howe, A., Patel, I., Wisely, B., LeVine, H., and McGeehan, G. M. (1991) J. Cell. Biochem. 15 (suppl.), 139
  40. Erdjument-Bromage, H., Lui, H., Lacomis, L., Grewal, A., Annan, R. S., McNulty, D. E., Carr, S. A., and Tempst, P. (1998) J. Chromatogr. 826, 167-181[CrossRef]
  41. Bickett, D. M., Green, M. D., Berman, J., Dezube, M., Howe, A. S., Brown, P. J., Roth, J. T., and McGeehan, G. M. (1993) Anal. Biochem. 212, 58-64[CrossRef][Medline] [Order article via Infotrieve]
  42. Williams, J. W., and Morrison, J. F. (1979) Methods Enzymol. 63, 437-467[Medline] [Order article via Infotrieve]
  43. Nemunaitis, J., Poole, C., Primrose, J., Rosemurgy, A., Malfetano, J., Brown, P., Berrington, A., Cornish, A., Lynch, K., Rasmussen, H., Kerr, D., Cox, D., and Millar, A. (1998) Clin. Cancer Res. 4, 1101-1109[Abstract]
  44. Helms, J. B., and Rothman, J. E. (1992) Nature 360, 352-354[CrossRef][Medline] [Order article via Infotrieve]
  45. Vey, M., Schäfer, W., Berghöfer, S., Klenk, H.-D., and Garten, W. (1994) J. Cell Biol. 127, 1829-1842[Abstract]
  46. Van Wart, H. E., and Birkedal-Hansen, H. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 5578-5582[Abstract]
  47. Grams, F., Huber, R., Kress, L. F., Moroder, L., and Bode, W. (1993) FEBS Lett. 335, 76-80[CrossRef][Medline] [Order article via Infotrieve]
  48. Grams, F., Reinemer, P., Powers, J. C., Kleine, T., Pieper, M., Tschesche, H., Huber, R., and Bode, W. (1995) Eur. J. Biochem. 228, 830-841[Abstract]
  49. Beynon, R. J., and Bond, J. S. (1989) in Proteolytic Enzymes: The Practical Approach (Beynon, R. J., and Bond, J. S., eds), pp. 76-78, IRL Press at Oxford University Press, Oxford
  50. Fox, J. W., and Bjarnason, J. B. (1995) Methods Enzymol. 248, 368-387[Medline] [Order article via Infotrieve]
  51. Pennica, D., Nedwin, G. E., Hayflick, J. S., Seeburg, P. H., Derynck, R., Palladino, M. A., Kohr, W. J., Aggarwal, B. B., and Goeddel, D. V. (1984) Nature 312, 724-729[Medline] [Order article via Infotrieve]
  52. Crowe, P. D., Walter, B. N., Mohler, K. M., Otten-Evans, C., Black, R. A., and Ware, C. F. (1995) J. Exp. Med. 181, 1205-1210[Abstract]
  53. Pandiella, A., Bosenberg, M. W., Huang, E. J., Besmer, P., and Massague, J. (1992) J. Biol. Chem. 267, 24028-24033[Abstract/Free Full Text]
  54. Mullberg, J., Oberthur, W., Lottspeich, F., Mehl, E., Dittrich, E., Graeve, L., Heinrich, P. C., and Rose-John, S. (1994) J. Immunol. 152, 4958-4968[Abstract/Free Full Text]
  55. Mullberg, J., Durie, F. H., Otten-Evans, C., Alderson, M. R., Rose-John, S., Cosman, D., Black, R. A., and Mohler, K. M. (1995) J. Immunol. 155, 5198-5205[Abstract]
  56. Arribas, J., and Massague, J. (1995) J. Cell Biol. 128, 433-441[Abstract]
  57. Arribas, J., Coodly, L., Vollmer, P., Kishimoto, T. K., Rose-John, S., and Massague, J. (1996) J. Biol. Chem. 271, 11376-11382[Abstract/Free Full Text]
  58. Loechel, F., Gilpin, B. J., Engvall, E., Albrechtsen, R., and Wewer, U. M. (1998) J. Biol. Chem. 273, 16993-16997[Abstract/Free Full Text]
  59. Peschon, J. J., Slack, J. L., Reddy, P., Stocking, K. L., Sunnarborg, S. W., Lee, D. C., Russel, W. E., Castner, B. J., Johnson, R. S., Fitzner, J. N., Boyce, R. W., Nelson, N., Kozlosky, C. J., Wolfson, M. F., Rauch, C. T., Cerretti, D. P., Paxton, R. J., March, C. J., and Black, R. A. (1998) Science 282, 1281-1284[Abstract/Free Full Text]
  60. Buxbaum, J. D., Liu, K. N., Luo, Y., Slack, J. L., Stocking, K. L., Peschon, J. J., Johnson, R. S., Castner, B. J., Cerretti, D. P., and Black, R. A. (1998) J. Biol. Chem. 273, 27765-27767[Abstract/Free Full Text]
  61. Giulian, D., Haverkamp, L. J., Yu, J., Karshin, W., Tom, D., Li, J., Kazanskaia, A., Kirkpatrick, J., and Roher, A. E. (1998) J. Biol. Chem. 273, 29719-29726[Abstract/Free Full Text]
  62. McGeehan, G., Bickett, D. M., Wiseman, J. S., Green, M., and Berman, J. (1995) in Proteolytic Enzymes: Aspartic and Metallo Peptidases (Barrett, A. J., ed), Vol. 248, pp. 35-46, Academic Press, San Diego
  63. Krätzschmar, J., Lum, L., and Blobel, C. P. (1996) J. Biol. Chem. 271, 4593-4596[Abstract/Free Full Text]
  64. Herren, B., Raines, E. W., and Ross, R. (1997) FASEB J. 11, 173-180[Abstract/Free Full Text]
  65. Shinde, U. P., Liu, J. J., and Inouye, M. (1997) Nature 389, 520-522[CrossRef][Medline] [Order article via Infotrieve]
  66. Suzuki, T., Yan, Q., and Lennarz, W. J. (1998) J. Biol. Chem. 273, 10083-10086[Free Full Text]


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