From the § Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146
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ABSTRACT |
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Mitochondrial cytochrome P450c27 (product of the
CYP27A1 gene) is found to have significantly higher affinity for the
common redox partner adrenodoxin than another mitochondrial P450,
P450scc (product of the CYP11A1 gene). To investigate the basis of the ~30-fold difference in adrenodoxin binding, two sets of P450c27 mutants were generated, expressed in Escherichia coli, and
purified. Mutations of one set were within the putative
adrenodoxin-binding site containing conserved lysine residues also
crucial in P450scc for binding adrenodoxin. The second set included
mutations within a sequence aligning with the "meander region" of
P450BM-3 proposed to be a site of redox-partner interactions in P450s
(Hasemann, C. A., Kurumbail, R. G., Boddupalli, S. S.,
Peterson, J. A., and Deisenhofer, J. (1995) Structure
3, 41-62). Mutation of the P450c27 conserved lysines (K354A and K358A)
led to a ~20-fold increase in apparent Ks for
adrenodoxin, confirming that these two positively charged residues
conserved in mitochondrial P450s are important for adrenodoxin binding.
Mutation of Arg-418, conserved in the CYP27A1 family, to serine also
decreased the affinity for adrenodoxin ~20-fold. This residue is
predicted to be located in the meander region. A triple
K354A/K358A/R418S mutation profoundly reduced adrenodoxin binding.
Thus, in contrast to P450scc, where mutation of the two conserved
positively charged residues results in virtually complete inhibition of
adrenodoxin binding, in P450c27 there are three of such residues
(Lys-354, Lys-358, and Arg-418) important for adrenodoxin interaction.
Cytochromes P450 (P450)1
are a large superfamily of hemoproteins found in bacteria, yeast,
fungi, nematodes, plants, insects, fish, and mammals (1). These
monooxygenases metabolize different endogenous and exogenous substrates
including steroids, fatty acids, drugs, and xenobiotics. Eukaryotic
P450s are membrane-bound enzymes residing either in the
endoplasmic reticulum or the inner mitochondrial membrane. Microsomal
P450s accept electrons from NADPH through NADPH-cytochrome P450
reductase (P450 reductase) while mitochondrial P450s receive electrons
from NADPH via a two-protein redox chain consisting of ferredoxin
reductase and ferredoxin. Whereas different forms of P450s are
necessary to metabolize different substrates, a single form of P450
reductase for microsomal P450s and ferredoxin for mitochondrial P450s
function as P450 redox partners.
Mammalian mitochondrial P450s are involved in the biosynthesis of
steroid hormones, the production of bile acids, and the formation of
active and inactive vitamin D3 metabolites. Among mitochondrial P450 systems that involved the conversion of cholesterol to pregnenolone (P450scc) in bovine adrenal cortex and other
steroidogenic tissues have been most extensively characterized because
of its relative abundance. Development of efficient Escherichia
coli expression systems leading to availability of large
quantities of adrenodoxin reductase (Adr) (2), adrenodoxin (Adx) (3) (the names of ferredoxin reductase and ferredoxin in adrenal glands) and P450scc (CYP11A1) (4), and the opportunity for site-directed mutagenesis, has greatly facilitated structure-function studies of
mitochondrial P450s. By site-directed mutagenesis, lysine residues at
positions 338 and 3422
(according to numbering of the mature protein) in P450scc were found to
play crucial roles in binding bovine Adx (4). The importance of these
residues for Adx binding was implicated initially by chemical
modification studies (5, 6). The fact that the key basic residues
identified in P450scc are conserved (as lysine or arginine) among all
mitochondrial but not in microsomal P450s (Fig.
1) suggests that a similar
ferredoxin-binding domain is present in all mitochondrial P450s.
INTRODUCTION
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Abstract
Introduction
References
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Fig. 1.
The alignment of amino acid sequences of
mitochondrial P450s in the region of the putative adrenodoxin-binding
site (4). Original references for these sequences can be found in
the P450 superfamily summary (1). Residues mutated in the previous (4)
and present studies are underlined. Two positively charged
lysines important for the interaction with Adx in mitochondrial P450s
are boxed in black. The outlined glutamate and
arginine are the part of the ERR triad (19).
Recently, we have overexpressed P450c27 (CYP27A1), another
mitochondrial P450, in E. coli (7). This ubiquitous enzyme
participates in bile acid biosynthetic pathways catalyzing multiple
oxidation reactions at the C-27 atom of steroids in the classical
(hepatic) pathway (8) and cholesterol in the alternative (peripheral) pathway (9). The present study was initiated by the observation that
the two mitochondrial P450s, P450scc and P450c27, show significantly different elution times from Adx-Sepharose, an affinity resin used for
purification of both. Quantitation of the binding affinities using a
spectral binding assay revealed an almost 30-fold difference in
apparent Ks values between these two P450s for
ferredoxin, with P450c27 binding more tightly. Utilizing site-directed
mutagenesis we have established the primary basis of this difference.
Three positively charged residues of P450c27 (Lys-354, Lys-358, and Arg-418) play essential roles in binding Adx. Two of them (Lys-354 and
Lys-358) are aligned with residues previously identified as the 2 residues essential for ferredoxin binding to P450scc (4), while Arg-418
is a newly identified residue important for the interaction of
mitochondrial P450c27 with Adx. This residue aligns with Ser-402 in
P450scc. The much tighter binding of P450c27 to Adx due to the presence
of the additional positively charged residue may be important because
of the low levels of ubiquitously expressed P450c27 and Adx relative to
much higher levels of P450scc and Adx in steroidogenic mitochondria.
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EXPERIMENTAL PROCEDURES |
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Materials--
Restriction endonucleases and other modifying
enzymes were purchased from Boehringer-Mannheim. Reagents for bacterial
growth were from Difco. [4-14C]Cholesterol and
[7-3H]pregnenolone were from DuPont, while
5-cholestane-3
,7
,12
-triol, [3H]5
-cholestane-3
,7
,12
-triol,
5
-cholestane-3
,7
,12
,27
-tetrol, and
[3H]5
-cholestane-3
,7
,12
,27
-tetrol were
kindly provided by Dr. I. Bjorkhem (Karolinska Institute, Sweden).
Other chemicals were purchased from Sigma. Recombinant bovine Adx and
Adr were expressed and purified as described previously (2, 3).
Site-directed Mutagenesis-- This was carried out using in vitro QuikChangeTM Site-directed Mutagenesis Kit (Stratagene) according to the instruction manual. Templates and complementary mutagenic oligonucleotides used to introduce mutations by polymerase chain reaction are shown in Table I. Correct generation of desired mutations was confirmed by DNA sequencing. The StuI/XbaI fragments of P450c27 and the BsmI/KpnI fragment of P450scc containing the mutations were ligated into StuI/XbaI- and BsmI/KpnI-digested expression constructs containing wild-type P450c27 and P450scc, respectively. For each P450c27 mutant the region from upstream of the StuI site to beyond the XbaI site (BsmI and KpnI sites for P450scc) was sequenced to make sure that there are no undesired mutations as well as to confirm correct ligation.
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Preparation of Mutant P450s--
Expression in E. coli was as described previously for wild-type P450s (4, 7),
except that E. coli strain DH5F'IQ (Life Technologies,
Inc.) was used, and chloramphenicol at a concentration of 1 µg/ml
culture was added at the same time as
isopropyl-1-thio-
-D-galactopyranoside and
-aminolevulinic acid. These changes were found to increase the level
of expression.3 The
purification procedure for all mitochondrial P450s was the same as
described for human recombinant P450c27 (7). After the last
purification step, chromatography on Adx-Sepharose, both wild types and
mutated forms of P450 were concentrated to 30 µM using
ultrafiltration membrane cones (Amicon), dialyzed overnight against 100 volumes of 40 mM phosphate buffer (pH 7.4) containing 20%
glycerol to remove 1 M NaCl and 0.5% sodium cholate
present in the elution buffer which interfere with spectral binding and enzymatic assays, and then frozen at
70 °C.
Spectral Studies--
Adrenodoxin-induced spectral changes of
P450s (spectral binding assay) were measured at room temperature as
described (10) using 0.6-4 µM P450 in 20 mM
potassium phosphate buffer (pH 7.4), 20% glycerol, 0.1% Tween 20, and
50 µM substrate (5-cholestane-3
,7
,12
-triol for P450c27 or cholesterol for P450scc). The concentration of free
ligand was calculated as described previously (11). After titration,
P450 content was measured by reduced CO-difference spectrum (12) to
confirm that there was no denaturation during titration.
Enzyme Assays--
Enzymatic activities of wild-type and mutated
forms of P450c27 were determined using
5-cholestane-3
,7
,12
-triol as a substrate as described
previously (7), except that a much higher molar excess of Adx
(140-fold, final concentration 5.6 µM) over P450 was
used. The reaction time was 10 min. To measure the activity of the
triple K354A/K358A/R418S P450c27 mutant, the reaction time was
increased to 20 min, and the final concentrations of the P450, Adx, and
Adr were 0.6, 22, and 1 µM, respectively. Enzymatic
activities of the wild-type and mutated forms of P450scc were
determined as described previously (13) using 50 µM
cholesterol as substrate in 40 mM phosphate buffer (pH 7.2)
containing 0.3% Tween 20. The reaction mixture (1 ml) contained 0.2 µM P450, 8 µM Adx, and 1 µM
Adr, and the reaction time was 10 min. Steroids were separated as
described previously after extraction with
CH2Cl2 and evaporation (7).
Matrix-assisted Laser Desorption Ionization Time of Flight Mass
Spectrometry (MALDI-TOF MS)--
Two microliters of wild-type P450c27
(250 µM in 30 mM potassium phosphate (pH
7.2), containing 15% glycerol) was mixed with 8 µl of ferulic acid
(10 mg/ml) in tetrahydrofuran, spotted on a MALDI-TOF sample plate, and
washed with 1% aqueous trifluoroacetic acid to remove glycerol. Mass
spectrometry was performed in a linear positive ion mode with a 350-ns
delayed pulse (linear delayed extraction mode) using a laser desorption
ionization mass spectrometer with a N2 pulse laser at 337 nm (PerSeptive Biosystems Voyager Elite). Signals from 255 excitation
pulses were accumulated using accelerating voltage, 25,000 V; grid
voltage, 94.0%; and guidewire voltage, 0.2%. Mass calibration was
performed using bovine serum albumin (average molecular weight is
66,431).
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RESULTS |
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Comparison of Binding of Wild-type P450c27 and P450scc to Adx-- To date, the mode of interaction of mitochondrial P450s with the redox partner Adx has only been investigated for bovine P450scc (4-6). When we overexpressed a second mitochondrial P450, human P450c27 in E. coli, affinity chromatography on the Adx-Sepharose column was used as a final purification step (7). P450c27 was found to elute from this column at a much higher salt concentration than P450scc. Since different detergents were used for solubilization of the mitochondrial P450s from the E. coli membranes, and different chromatographic steps preceded chromatography on Adx-Sepharose P450scc (4) and P450c27 (7), it was possible that these differences affected chromatographic behavior on Adx-Sepharose. To test this possibility, recombinant P450scc was purified exactly in the same way as P450c27 and applied to Adx-Sepharose. Changing the purification protocol did not affect the elution time of P450scc from Adx-Sepharose. It is eluted as one peak at 0.54 M NaCl, 0.34% sodium cholate, while P450c27 is eluted as two overlapping peaks, one at 0.9 M NaCl, 0.47% sodium cholate and the other at 1 M NaCl, 0.5% sodium cholate (Fig. 2). P450c27 in the two peaks has identical spectral parameters in the region between 260 and 715 nm, and identical electrophoretic mobility and purity as judged by the silver-stained SDS-polyacrylamide gel electrophoresis (data not shown). The two peaks were pooled and subjected to MALDI-TOF MS to check if there is any heterogeneity in the fraction. Three peaks are seen in the MALDI-TOF mass spectrum of P450c27 (Fig. 3). The first and the second peaks correspond to doubly and singly charged monomers of P450c27, respectively, and the third peak is a singly charged dimer of the protein which is a laser induced artifact of MALDI-TOF MS. The molecular weight of the singly charged monomer of P450c27 was 56,909 while the theoretical value of 56,910 is calculated from the primary sequence. The results of MALDI-TOF MS rule out heterogeneity of the sample due to post-translational modifications of P450c27 or its proteolytic digestion during purification. One possible explanation for the two-peak elution profile of P450c27 from Adx-Sepharose is based on our observation that in solution in the absence of detergent, P450c27 forms aggregates with an average molecular mass of 1,146 kDa (data of light scattering),4 approximately 20 molecules per aggregate. As a result of the aggregation, not all the residues important for binding Adx in P450c27 molecule may be available for interaction with immobilized Adx because of steric hindrance. The peak eluted first from Adx-Sepharose could result from nonspecific aggregation of P450c27 having only some of these residues available for Adx interaction. The second peak could be aggregates in which all residues in P450c27 important for the binding to Adx are available for interaction.
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Chromatography on Adx-Sepharose clearly shows that there are
significant differences in affinities between the substrate-free forms
of two mitochondrial P450s. To quantitate these differences, Adx
binding constants were estimated using a spectral binding assay. As
shown earlier (14), binding of adrenodoxin to P450scc in the presence
of substrate (cholesterol) and the nonionic detergent Tween 20 induces
a change in spin state of the heme iron and a shift in the absorbance
from 418 to 389 nm. When cholesterol is absent, the Adx-induced spin
state change is much smaller (14). The value of the apparent binding
constant, Ks, is obtained from double reciprocal
plots of the concentration of free Adx and the differences in
absorbance change upon titration with Adx (Fig.
4). Ks and hence the
interaction between P450scc and Adx is found to be affected by several
factors. Presence of cholesterol increases the affinity of P450scc for
Adx (13-20-fold) (14), while binding of Adx to both substrate-free
P450scc and the cholesterol complex is significantly reduced as ionic
strength is increased (10, 14). Binding is unaffected by the nonionic detergent Tween 20 (14). The fact that the Ks value for Adx is substrate-dependent means that apparent binding
constants of different mitochondrial P450s determined in the presence
of different substrates may not accurately reflect physiological differences in the affinity of these P450s for Adx. P450c27 has a wide
substrate specificity, the best physiological substrate being
5-cholestane-3
,7
,12
-triol (15). Cholesterol is also an
important physiological substrate, although the rates of metabolism by
P450c27 are much slower than those for the triol substrates. Titrations
of recombinant P450c27 with up to 10 µM Adx in the presence of 0.1% Tween 20 and either 50 µM
5
-cholestane-3
,7
,12
-triol, or 50 µM
cholesterol, or without any substrate showed spectral changes only in
the presence of 5
-cholestane-3
,7
,12
-triol. Thus the
titration buffer (20 mM potassium phosphate, 20% glycerol, 0.1% Tween 20) for P450scc contained 50 µM cholesterol
while that for P450c27 contained 50 µM
5
-cholestane-3
,7
,12
-triol. The apparent binding constants
of Adx for recombinant P450c27 and recombinant P450scc were determined
to be 0.049 and 1.42 µM, respectively (Table
II), confirming the original
chromatographic observation that P450c27 binds much more tightly to Adx
than P450scc. We believe that the almost 30-fold difference in
Ks values between two P450s rather accurately
depicts the difference in affinities to Adx-Sepharose (Fig. 2), and two
independent methods (chromatography on Adx-Sepharose and spectral
binding assay) can be used for evaluation of the effect of amino acid
substitutions in P450s on their binding properties to Adx.
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Selection of Amino Acid Residues for Mutation--
Calculation of
the difference in free energy of binding, (
G), between
P450scc-Adx, and P450c27-Adx using spectral binding constants permits
prediction of the type of additional protein-protein interactions which
might be involved in formation of the P450c27·Adx complex. While
dependent on the environment and solvent exposure, the value of 1.97 kcal/mol indicates that it could be either one additional salt bridge
(while environmental dependent, the disruption of electrostatic
interaction between charged groups is reported to weaken binding energy
by about 2.0 kcal/mol (16)), or more than one hydrogen bond, involving
non-polar groups (the weakening of binding energy in this case is
0.5-1.5 kcal/mol (17)). Previous site-directed mutagenesis studies of
human Adx identified two negatively charged residues (Asp-76 and
Asp-79) as being important for high affinity binding to bovine P450scc
(18). These 2 residues are located in a region (residues 68-86) within
mitochondrial ferredoxins containing 5 additional acidic residues that
could also potentially be involved in the interaction with P450c27. Based on the fact that much higher ionic strength is needed to elute
P450c27 from Adx-Sepharose, we surmised that stronger binding between
P450c27 and Adx compared with the P450scc-Adx pair is due to an
additional electrostatic interaction between the two proteins involving
a positive charge on P450c27 and a negative one on Adx. Alignment of
amino acid sequences in the region which is highly conserved among
mitochondrial P450s containing the two lysine residues crucial for the
interaction of P450scc with Adx (Lys-338 and Lys-342) (Fig. 1) shows
that only three amino acid residues at positions 356, 357, and 364 (P450c27 numbering) are different between human P450c27 and bovine
P450scc, and none of these are positively charged. The conserved
Lys-354 and Lys-358 were converted to Ala to determine whether they are
also important for the binding to Adx in P450c27. We also mutated each
of 3 residues at positions 356, 357, and 364 in P450c27 to the
corresponding residues in P450scc in addition to generating a triple
V356S/L357I/Y364H P450c27 mutant making the complete stretch (residues
351-365) in P450c27 identical to that in P450scc.
The putative Adx-binding site in mitochondrial P450s also contains the glutamate and arginine residues (Glu-359 and Arg-362 in P450c27, Fig. 1) which are invariant in all P450 sequences. In three soluble bacterial P450s with known tertiary structure, these invariant glutamate and arginine are part of the so called ERR triad in which the glutamate forms salt bridges with both arginines located 2 and ~50 residues away from it in the P450 primary sequences (19). The second arginine in the ERR triad is located in the meander region (Arg-415 in P450c27 aligns with the conserved ERR arginine in the meander region, Fig. 5) and in different P450s it is arginine, histidine, or asparagine (19). The meander region (~20 amino acids) has been proposed to be a site of interaction with redox partners in P450s (19). Despite more than 50 amino acids separating the first 2 residues in the ERR triad from the last one, they are spatially very close in the three bacterial P450s forming a set of salt bridge interactions between each other and carbonyl oxygens in the meander (19). This implies that in mitochondrial P450s the regions containing residues of the ERR triad (the conserved Adx-binding site (Fig. 1) containing the first 2 residues of the ERR triad and the meander region containing the last residue of the ERR triad, Fig. 5) may be spatially close, and that the meander region could contain residues which interact with Adx. Alignment of mitochondrial P450s in the meander region (Fig. 5) shows that Arg-418 is conserved within the CYP27A1 family but is not conserved among all mitochondrial P450s. This arginine was mutated to serine in P450c27. Later, after obtaining data on properties of this serine mutant, a reciprocal mutant of P450scc, S402R, and additional mutants of P450c27, H414T, and K354A/K358A/R418S were generated.
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Expression and Purification of Mutant P450s-- All the mutants except two were overexpressed at levels comparable with the wild-type enzymes. Immunoblotting analysis using antiserum against human recombinant P450c27 showed no detectable protein after the expression of the Y364H P450c27 and V356S/L357I/Y364H P450c27 mutants in E. coli (data not shown). Thus of the 10 mutants only eight were studied. The last purification step, chromatography on Adx-Sepharose, was also used to monitor the affinity of the substrate-free forms for Adx in the absence of nonionic detergent. Three single P450c27 mutants, K354A, K358A, and R418S, were eluted much earlier from Adx-Sepharose than wild type (Fig. 2C), indicating alteration of Adx binding properties. The elution of these mutants was close to that of wild type P450scc (Fig. 2A). When the triple K354A/K358A/R418S P450c27 mutant was subjected to chromatography on Adx-Sepharose, ~50% of the amount applied was found in the flow-through fraction, while the rest, bound to the resin, was eluted much earlier than any of the single mutants (Fig. 2C). The fact that a portion of K354A/K358A/R418S P450c27 mutant bound to Adx-Sepharose may indicate that besides Lys-354, Lys-358, and Arg-418 there are some other residues in P450c27 involved in the binding to Adx. However, because very low ionic strength was needed to elute a triple mutant from Adx-Sepharose, the total contribution of such residues appears to be much smaller than those of Lys-354, Lys-358, or Arg-418. The flow-through portion of the K354A/K358A/R418S P450c27 mutant may represent nonspecific aggregates in which these additional residues are not available for interaction with Adx-Sepharose. The elution profiles of the other single P450c27 mutants, V356S, L357I, and H414T, showed modest changes in binding to the column compared with the wild type (Fig. 2B). The reciprocal S402R P450scc mutant was generated to determine if this substitution will improve the interaction of P450scc with Adx. Chromatographic behavior of this mutant on Adx-Sepharose, however, indicated it actually binds more weakly to Adx-Sepharose than wild type (Fig. 2A).
All purified mutants exhibited UV and visible absorption spectra indistinguishable from wild-type P450s. They all were isolated as substrate-free, low spin forms with specific heme contents of 16.5-17.5 nmol/mg of protein, except the triple mutant, K354A/K358A/R418S, which had lower heme content (9.2 nmol/mg of protein in the flow-through and 11.4 nmol/mg of protein for the fraction bound to Adx-Sepharose). After chromatography on hydroxylapatite, which precedes chromatography on Adx-Sepharose, the specific heme content of the K354A/K358A/R418S P450c27 mutant was 9.8 nmol/mg of protein, a value close to that obtained for the wild-type P450c27 at this step (7). The lack of significant enrichment of specific heme content of the fraction eluted from Adx-Sepharose accompanied by no significant change of specific heme content of the flow-through fraction indicates that simultaneous replacement of 3 residues did not cause major structural perturbations resulting in the formation of apoprotein or denatured protein. No P420 form was detected in any of the purified mutants.
Spectral Assay of Adx Binding--
Addition of adrenodoxin to
purified P450 mutants produced difference spectra qualitatively similar
to those elicited by the wild-type enzymes allowing determination of
apparent binding constants summarized in Table II. The double
reciprocal plots for titration of wild-type and mutated forms of
P450scc and P450c27 are shown in Fig. 4. Approximately the same
increase in Ks values (20-fold) was obtained for
each of the three single P450c27 mutants (K354A, K358A and R418S)
corresponding to an increase in apparent free energy of binding
(
G) of ~1.7 kcal/mol (Table II). These data are
consistent with results from chromatography on Adx-Sepharose and
suggest that all three positively charged residues at positions 354, 358, and 418 are important for interaction with Adx. The other three
P450c27 mutants (V356S, L357I and H414T) showed slight decreases
(2-3.5-fold) in apparent binding constants with only small decreases
of apparent free energy of binding
(
G) of 0.4-0.7 kcal/mol (Table II). Only the triple K354A/K358A/R418S P450c27 mutant
(both bound and unbound to Adx-Sepharose fractions) did not show any
spectral shift even upon addition of a very high concentrations of Adx
(up to 36 µM), or when the concentration of the P450 used
in the spectral binding assay was increased 7-fold (data not shown).
Mutation of serine to arginine in P450scc at position 402 which was
predicted to increase the affinity for Adx based on studies with
P450c27, in contrast led to a 2.6-fold increase in
Ks value (Table II).
Enzyme Assays--
The mutant forms of P450c27 and P450scc were
also tested for 27-hydroxylation of
5-cholestane-3
,7
,12
-triol and side chain cleavage activity
of cholesterol, respectively, by reconstitution with Adx and Adr.
Activity in this assay requires binding of Adx to Adr and P450 and
productive electron transfer. These experiments were carried out using
a 140-fold molar excess of Adx over P450 to compensate for the
differences in affinity of Adx for different mutant P450s. To measure
the specific activity of the triple K354A/K358A/R418S P450c27 mutant
(both unbound and bound to Adx-Sepharose fractions) a 15-fold higher
concentration of P450 was used and the reaction time was 2 times
longer. All mutants except the unbound fraction of the triple
K354A/K358A/R418S P450c27 mutant showed enzymatic activities but had
reduced turnover numbers (Table II).
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DISCUSSION |
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Details of the interactions of different mitochondrial P450s with their common redox partner ferredoxin are derived from chemical modification and site-directed mutagenesis studies using bovine P450scc. Finding that the key basic residues for interaction with Adx in P450scc are conserved among all mitochondrial P450s suggests that this interaction involves a similar conserved binding domain. In the present study it is confirmed with a second mitochondrial P450, human P450c27, that these conserved lysine residues are important for Adx binding. However, it is also shown that an additional positively charged residue (Arg-418) participates in Adx binding, leading to tighter binding of Adx to P450c27 than to P450scc. This residue is located outside the immediate environment of the conserved lysine residues.
Binding of Adx to P450c27 exhibits several differences compared with that to P450scc. First, it is 30-fold tighter. Despite this, the contribution of each of the conserved lysines (Lys-354 and Lys-358) is approximately an order of magnitude weaker than in P450scc. For example, in P450scc mutation of K338A resulted in about a 250-fold increase in apparent Ks value, while the replacement of the corresponding lysine residue in P450c27 (K354A) led to only about a 20-fold change of Ks. A smaller 150-fold increase in Ks was observed upon the mutation of the second conserved lysine in P450scc (K342Q) with ~20-fold increase in Ks arising from mutation of the corresponding K358A in P450c27. The finding that P450c27 binds more tightly to Adx than P450scc and that the conserved lysine residues in P450c27 play a smaller role in Adx binding than in P450scc suggests that additional residue(s) in P450c27 participate in the interaction with Adx. At first we checked the effect of differences in the primary sequence between the two P450s in the 15-amino acid stretch containing the conserved lysine residues important for Adx binding. Between bovine P450scc and human P450c27 there are three differences in this stretch (Val-356, Leu-357, and Tyr-364) and replacement of Val-356 and Leu-357 in P450c27 with the corresponding residue in P450scc (Fig. 1) showed no significant effect on Adx binding. Neither the Y364H nor V356S/L357I/Y364H mutants were detected using immunoblotting analysis during expression in E. coli. Apparently Tyr-364 in P450c27 is important for maintaining structural integrity of the protein, and P450c27 cannot be properly folded when this residue is replaced with histidine, the residue present in P450scc. Mutation of V356S and L357I actually slightly improved binding of Adx to P450c27 indicating that these 2 residues do not contribute to tighter complex formation between Adx and P450c27. Despite the fact that affinity to Adx was increased, the V356S and L357I mutants showed moderately decreased enzymatic activity implying that these mutations directly or indirectly affect some other steps in the P450 catalytic cycle. Because Val-356 and Leu-357 are located within the Adx-binding site, their mutation could affect the rate of electron transfer and hence the turnover number. Thus, while the conserved lysine residues are important for Adx binding to P450c27, additional residues essential for this are located outside this region.
To identify these additional residues we utilized the results of a
comparative analysis of crystal structures of three bacterial P450s
(19). This analysis argues that the general structure of eukaryotic
P450s resembles that of the bacterial enzymes, and most relevant to
this study identifies the ERR triad and the meander region as typical
features of P450 structure (19). The meander region was proposed to be
a likely site of redox-partner interactions (19). Mutation of the
positively charged Arg-418, predicted to be located in the P450c27
meander region and conserved in the CYP27A1 family decreased the
affinity of P450c27 to Adx ~20-fold, a comparable effect to that
caused by the mutation of conserved Lys-354 and Lys-358. This mutant
was expressed at the same level as the wild-type enzyme, did not
exhibit any abnormalities during purification, showed a peak at 448 nm
without any trace of a peak at 420 nm in the reduced CO-difference
spectrum and was able to support the activity in the reconstituted
system, although full activity was not achieved because of the lower
affinity to Adx. This mutation did not cause any major structural
perturbations in the protein and the substantial decrease of affinity
for Adx is most likely the result of the neutralization of a positive charge at position 418. The 1.7 kcal/mol increase in apparent free
energy of binding caused by this mutation is not significantly different from the value of 1.97 kcal/mol, characterizing
(
G) between P450scc-Adx and P450c27-Adx. Such close
correspondence of these two values implies that Arg-418 is the most
important among the residue(s) in P450c27 which confer(s) the
additional strength for Adx binding. Mutation of His-414 which is also
located in the meander region, only 3 residues away from Arg-418, to
Thr the corresponding residue in P450scc, did not decrease but slightly improved binding to Adx, indicating that His-414 is not the additional residue in P450c27 important for the interaction with Adx.
The present studies raise new questions regarding the interaction of
mitochondrial P450s with the common ferredoxin redox partner. The most
important one is: what is the physiological significance of greatly
different affinities of mitochondrial P450s for ferredoxin? Besides the
present work demonstrating that two mitochondrial P450s have
significantly different Adx-binding properties, there are several
studies showing that mitochondrial P45011 (CYP11B1) has lower
affinity for Adx than P450scc (11, 20, 21). Thus, differences in
binding affinity for ferredoxin may be a general phenomenon among
mammalian mitochondrial P450s. Perhaps the different levels of
expression of ferredoxin in different tissues and the tissue
distribution pattern of mitochondrial P450s provide an explanation. The
lower the level of expression of ferredoxin and P450 in a given tissue
(liver or kidney, for example) the higher the affinity between two
proteins and vice versa. In tissues where several mitochondrial P450s
are present simultaneously at relatively high levels (P450scc and
P45011
in adrenal cortex), different affinities may serve to
regulate the preferential partitioning of electron transfer toward one
P450. If the level of expression of two P450s is significantly
different, higher binding affinity of the more poorly expressed P450 is
a way to compete efficiently for ferredoxin.
Another unanswered question is how many binding sites for Adx are in mitochondrial P450s, and if there are several sites, are they formed by the same stretch of primary sequence in different mitochondrial P450s? In the absence of the tertiary structure of P450c27 we do not know if Lys-354, Lys-358, and Arg-418 are part of one site. If the overall tertiary structure of P450c27 is not too different from that of bacterial P450s, P450cam, P450terp, and P450BM-3, then these 3 residues should be spatially close to each other forming a single binding site. Our data also indicates that there may be other residues in P450c27 interacting with Adx which form a separate binding site, which contributes much less to Adx binding than Lys-354, Lys-358, or Arg-418. Thus, in mitochondrial P450s with high affinity for Adx there may be two sites for interaction with Adx, one being common and the other one being specific for each P450.
Site-directed mutagenesis studies have shown that four conserved
negatively charged residues (Asp-72, Glu-73, Asp-76, and Asp-79)
located within the highly acidic region (positions 68-86) in
mitochondrial ferredoxins are important for binding of human ferredoxin
to bovine P450scc (18). The contribution of these residues to the
affinity for P450scc is different being the greatest for Asp-76 and
Asp-79 (Ks for D76N and D79A mutants was increased
~6- and 8-fold, respectively) followed by Asp-72 and Glu-73 (3- and
4-fold increase of Ks for D72N and E73Q mutants)
(18). Recently the tertiary structure of a truncated form of bovine Adx
where 3 residues from the N terminus and 20 residues from the C
terminus were removed by genetic engineering has been determined (22).
This truncated form exhibits 3.2- and 6-fold decreased
Km values for P450scc and P45011, respectively,
compared with that of the wild type, and 4.5-fold enhanced efficiency
of substrate conversion catalyzed by P45011
(23). Subsequently we
determined the tertiary structure of full-length bovine
Adx.5 Residues Asp-72,
Glu-73, Asp-76, and Asp-79 are located on the surface of the molecule
in both structures with their side chains directed toward the solvent.
The carbon atoms of the carboxylate groups of these 4 residues form the
corners of a quadrilateral with length of the sides between 6.6 and 9.1 Å. Thus, 4 acidic residues in adrenodoxin are potentially available
for the pairwise electrostatic interactions with mitochondrial P450s.
Determination of which of the residues are paired in electrostatic
interactions with P450c27 and P450scc by analysis of the interactions
of mutant forms of both partners could establish whether differences in paired interactions exist between mitochondrial P450s which contribute to both binding and catalytic activity.
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ACKNOWLEDGEMENTS |
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We thank Dr. V. Q. Nguyen for carrying out MALDI-TOF MS of P450c27 and Dr. R. M. Caprioli for discussion of mass spectrometry techniques and data.
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FOOTNOTES |
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* This work was supported in part by United States Public Health Service Grants P30 ES00267 (pilot project funds to I. A. P.) and GM37942 (to M. R. W.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Biochemistry,
Vanderbilt University School of Medicine, Nashville, TN 37232-0146. Tel.: 615-343-1373; Fax: 615-322-4349; E-mail: pikuleva{at}toxicology.mc.vanderbilt.edu.
The abbreviations used are:
P450, cytochrome
P450; P450scc, cholesterol side chain cleavage cytochrome P450, product
of the CYP11A1 gene; P450c27, cytochrome P450 catalyzing the multiple
oxidation reactions at C-27 of steroids, product of the CYP27A1 gene; P450cam, cytochrome catalyzing the stereospecific hydroxylation of the
camphor, product of the CYP101 gene; P450terp, cytochrome catalyzing
the oxidation of the -terpinol, product of the CYP108 gene; P450BM-3, cytochrome catalyzing the oxidation of a variety of fatty
acids, product of the CYP102 gene; P45011
, cytochrome P450
catalyzing the 11
-hydroxylation of 11-deoxycortisol to cortisol and
the production of aldosterone, product of the CYP11B1 gene (1); Adx, adrenodoxin; Adr, adrenodoxin reductase; MALDI-TOF MS, matrix-assisted
laser desorption ionization time of flight mass spectrometry.
2 Lys-377 and Lys-381 are according to numbering of the precursor form of P450scc which is used in Ref. 4. In the present paper, numbering of the residues is based on their positions in the primary sequence of the mature forms of P450scc and P450c27.
3 K. Kusano, unpublished data.
4 I. A. Pikuleva, C. Cao, and M. R. Waterman, unpublished data.
5 I. Pikuleva, manuscript in preparation.
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REFERENCES |
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