From the Department of Biology, Kyushu University
Faculty of Science, Fukuoka 812-8581, Japan and § CREST,
Japan Science and Technology Corporation, Tokyo 170-0013, Japan
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ABSTRACT |
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Rat cDNA encoding a 376-amino acid peroxin
was isolated by functional complementation of a peroxisome-deficient
Chinese hamster ovary cell mutant, ZP110, of complementation group 14 (CG14). The primary sequence showed 28 and 24% amino acid identity
with the yeast Pex14p from Hansenula polymorpha and
Saccharomyces cerevisiae, respectively; therefore, we
termed this cDNA rat PEX14 (RnPEX14). Human
and Chinese hamster Pex14p showed 96 and 94% identity to rat Pex14p,
except that both Pex14p comprised 377 amino acids. Pex14p was
characterized as an integral membrane protein of peroxisomes, exposing
its N- and C-terminal parts to the cytosol. Pex14p interacts with both
Pex5p and Pex7p, the receptors for peroxisome targeting signal type 1 (PTS1) and PTS2, respectively, together with the receptors' cargoes,
PTS1 and PTS2 proteins. Mutation in PEX14 from ZP161, the
same CG as ZP110, was determined by reverse transcription-PCR as
follows. A 133-base pair deletion at nucleotide residues 37-169 in one
allele created a termination codon at 40-42; in addition to this
mutation, 103 base pairs were deleted at positions 385-487, resulting
in the second termination immediately downstream the second deletion
site in the other allele. Neither of these two mutant forms of Pex14p
restored peroxisome biogenesis in ZP110 and ZP161, thereby
demonstrating PEX14 to be responsible for peroxisome deficiency in CG14.
The peroxisome is a ubiquitous, spherical intracellular organelle
bounded by a single membrane and with a diameter of 0.1-1 µm.
Peroxisomes are present in eukaryotic cells, from yeast to humans.
Peroxisomes function in a wide variety of metabolic pathways, including
We herein isolated rat, human, and Chinese hamster PEX14,
which restored peroxisome biogenesis in a CHO cell mutant ZP110, using
a genetic complementation cloning strategy (17-22). Pex14p is a
peroxisomal integral membrane protein, apparently involved in PTS1 and
PTS2 protein import mediated by Pex5p and Pex7p, respectively.
Rat Liver cDNA Library and Search for Complementing
cDNA--
Rat liver cDNA library in a ZAP Express predigested
vector (Stratagene, La Jolla, CA), containing unidirectionally inserted cDNA under the CMV promoter, was described (20, 21). The library was divided into small pools, each containing about 2,000 clones. Combined cDNA pools consisted of an equal amount of three small pool mixtures, containing approximately 6,000 independent clones. For
transfection of a cDNA library, ZP110 cells were cultured in Ham's
F-12 medium supplemented with 10% fetal calf serum. Cells that had
been plated on a coverslip 1 day before transfection at 1 × 105 cells/well of a six-well plate were washed twice with
serum-free F-12 medium. One µg of plasmid DNA and 12 µg of
Lipofectamine (Life Technologies, Inc.) were separately diluted with
100 µl of serum-free medium, Opti-MEM (Life Technologies). Both
solutions were mixed and left for 30 min at room temperature before
pouring onto the cells. After incubation for 1 h at 37 °C, the
DNA-liposome mixture was removed by aspiration, and fresh F-12 medium
with fetal calf serum was added. The cells were cultured for 2 days and
further incubated overnight in 2 ml of serum-free F-12 medium. Among
cDNA pools examined, a positive one (A9) that restored peroxisomes in ZP110 was further divided into subpools and screened. A cDNA fragment (SalI-NotI fragment) of an isolated
clone pBK-CMV·PAF-4, was blunted and subcloned into the
SmaI site of pBluescript II KS( Screening of Human and Chinese Hamster cDNA Library--
A
32P-labeled, 0.65-kilobase pair BamHI fragment
of open reading frame from rat (Rattus norvegicus;
Rn) PEX14, RnPEX14, was used as a
probe to screen 2.4 × 105 colonies of human liver
cDNA library (16) in pCMVSPORT I (Life Technologies). Three
positive clones were isolated; a longer one was sequenced. About
1.5 × 105 independent colonies of a cDNA library
from wild-type CHO-K1 cells (14) were also screened with a
32P-labeled RnPEX14 (0.85-kilobase pair
SalI-EcoRI) fragment. Two of 10 positive clones
were subcloned into pBluescript II SK( Transfection of PEX14--
An expression plasmid
pUcD2Hyg· RnPEX14 was generated by inserting a
SalI-ApaI fragment (nucleotide residues at Morphological Analysis--
Peroxisomes in CHO cells and human
fibroblasts were visualized by indirect immunofluorescence light
microscopy, under a Carl Zeiss Axioskop FL microscope (Oberkochen,
Germany) using a number 17 filter, as described by Tateishi et
al. (15). Antibodies used were rabbit antibodies to rat liver
catalase (11), human catalase (4), PTS1 peptide (14), 70-kDa
peroxisomal integral membrane protein (PMP70) (11). Anti-Pex14p
antibody was raised in rabbits by immunizing with synthetic peptide
comprising the C-terminal, 19-amino acid sequence of rat Pex14p (see
Fig. 2, dashed underline) supplemented with
cysteine at the N terminus that had been linked to keyhole limpet
hemocyanin (17). Antigen-antibody complex was detected using
fluorescein isothiocyanate-labeled sheep anti-rabbit immunoglobulin G
antibody (Cappel, Durham, NC), under a Carl Zeiss Axioskop FL microscope.
Mutation Analysis--
Total RNA was obtained from ZP110 and
ZP161 cells, by the acid-guanidinium-phenol-chloroform method (23).
Poly(A)+ RNA was isolated using Oligo(dT)-Latex (Takara,
Tokyo, Japan). Reverse transcription-PCR using poly(A)+ RNA
was done with a pair of ClPEX14-specific PCR primers,
a sense f1 (5'-CCACTGCAGAGGAAGATGGCGTCC-3' (initiation
codon, underlined) and an antisense r1
(5'-GCCCACCTGCCACTGATG-3') to cover a full length of
PEX14 open reading frame. PCR products were cloned into pBluescript II SK( Expression of Epitope-tagged Pex14p--
An epitope,
myc, tagging to the C terminus of Pex14p was done
with a PCR-based technique, using a forward primer
(5'-CTGGATCCTGGAGTC-3') and a reverse primer
(5'-CCGGGTACCTCACAAGTCTTCTTCAGAAATCAGCTTTTGTTCGTCTCGCTCACTCTC-3') containing the myc epitope (underline) and a stop
codon. myc-tagged RnPEX14 was cloned into
pBluescript II KS( Subcellular Fractionation--
Liver was excised from a
7-week-old male Wistar rat and homogenized, as described by Miura
et al. (24), except that 1 µM each of
pepstatin and leupeptin was added to a homogenizing buffer. A
postnuclear supernatant fraction, prepared by centrifugation of the
liver homogenates at 750 × g for 10 min, was then
centrifuged at 2,500 × g for 10 min, 25,000 × g for 10 min, and 100,000 × g for 1 h
to separate heavy and light mitochondria, microsomes, and cytosol,
respectively (25, 26). The light mitochondrial fraction (12 mg of
protein) was further subjected to sucrose density gradient
ultracentrifugation (24). Protein and marker enzymes, catalase for
peroxisomes, glutamate dehydrogenase for mitochondria, esterase for
microsomes, and N-acetyl- Expression of Fusion Protein--
Glutathione
S-transferase (GST) fusion protein with rat Pex14p was
constructed in Escherichia coli expression vector pGEX4T-1 (Amersham Pharmacia Biotech) as follows. The 3'-half of the
PvuII fragment of pBS-RnPEX14 was inserted into
the SmaI site of pGEX4T-1. This vector expresses the
C-terminal region of Pex14p, termed GST-C. The 5'-half of
RnPEX14 was amplified using PCR primers (forward,
5'-GCCAGATCTGCGTCCTCGGAGCAG-3'; reverse, 5'-TCTTCATCCACGTGGCTCAC-3'). GST-Pex14p-expression plasmid was constructed by replacing the BamHI fragment of GST-C with the
BglII-BamHI fragment of the amplified 5'-half
products of RnPEX14. GST fusion protein with a shorter isoform of Pex5p (Pex5pS) and a longer, 37-amino acid-inserted isoform
Pex5p (Pex5pL) of Chinese hamster (14) was likewise expressed in
E. coli.2
His6-tagged human Pex7p-expression vector was constructed
as follows. Blunted SmaI-SalI fragment of human
PEX7 in pTZ18R
(28)3 was ligated into the
blunted SalI site of E. coli expression vector
pQE30, containing a histidine tag sequence (QIAGEN, Hilden, Germany).
Purification of Fusion Protein--
Five hundred µl each of
16-h culture of E. coli, separately expressing GST and GST
fusion protein, were diluted with 10 volumes of YT medium at 2-fold
concentration. After 1 h of culture at 37 °C,
isopropyl-
His-Pex7p was isolated as follows. His6-tagged
Pex7p-expressing E. coli cells were sonicated, and inclusion
bodies were isolated by centrifugation. After solubilized with 8 M urea, His-Pex7p was purified by affinity chromatography
using a nickel-nitrilotriacetic acid-agarose column (QIAGEN), where
elution was done with 0.5 M imidazole, 8 M
urea, according to the procedure recommended by the manufacturer.
His-Pex7p was used at a 10-20-fold dilution.
In Vitro Binding Assay--
Binding reaction mixture contained
protein components to be examined, including 20 µg of GST or
GST-Pex14p, His-Pex7p (5 µg), and 35S-labeled
3-ketoacyl-CoA thiolase (29) (5 µl) in the binding assay buffer
(400-500 µl) described above. The reaction was done overnight at
4 °C. A binding reaction was also done, using GST-Pex14p (3 µg),
each of the two isoforms of Pex5p (3 µg) (i.e. Pex5pS or
Pex5pL), 35S-labeled rat acyl-CoA oxidase (AOx) (30) (5 µl), GST-Pex14p (20 µg), and 35S-Pex14p (5 µl).
Proteins were recovered using glutathione-Sepharose, washed four times
with the binding assay buffer minus BSA, and analyzed by SDS-PAGE using
12% or 4-20% polyacrylamide gel. Radioactive protein bands were
detected using a FujiX BAS1000 Bio-Autoimazing Analyzer (Fuji Photo
Film, Tokyo, Japan), and His-Pex7p and Pex5p were detected by
immunoblot using mouse monoclonal anti-RGS-His tag antibody (QIAGEN)
and rabbit anti-Chinese hamster Pex5pS
antibody,4 respectively.
Other Methods--
Continuous cell labeling with
[35S]methionine and [35S]cysteine was done
by culturing cells for 24 h in a medium containing 20 µCi/ml
[35S]methionine and [35S]cysteine (Amersham
Pharmacia Biotech). In vitro transcription/translation (30)
were performed as described. Immunoprecipitation of proteins using
antibodies to rat AOx and 3-ketoacyl-CoA thiolase and a catalase
latency assay using digitonin were performed as described (11). For
Northern blotting, RNA was isolated from CHO cells and the liver of a
rat treated for 7 days with clofibrate and of an untreated rat. The
blot was hybridized with the BamHI fragment (nucleotide
residues at 232-605) of RnPEX14 labeled with
[ Cloning of a Rat PEX14 cDNA
We used a transient expression assay as a cDNA cloning
strategy (16, 18, 20, 21) on a CG14 CHO cell mutant, ZP110, defective
in peroxisome assembly (15) (Fig.
1a). A cDNA library divided into small pools was transfected to ZP110. Peroxisome-restoring positive cDNA clones were searched for by immunofluorescence
staining of catalase, a peroxisomal matrix marker enzyme. One combined pool (A9) yielded several peroxisome-restored cells of ZP110, in a
single dish (Fig. 1b, arrows). After a fourth
round of screening, one positive clone, named pBK-CMV·PAF-4, was
isolated, which restored peroxisomal import of catalase in ZP110 (data
not shown). The cDNA portion of pBK-CMV·PAF-4 was sequenced on
both strands, indicating that the cDNA was 1,921 bp in length with
an open reading frame encoding a protein consisting of 376 amino acids
(Fig. 2). The calculated molecular mass
of its deduced amino acid sequence was 40,936 Da. The amino acid
sequence showed 28 and 24% identity with those of Pex14p from
Hansenula polymorpha (31) and Saccharomyces cerevisiae (32, 33), respectively (Fig. 2). Thus, we termed this
cDNA rat PEX14, RnPEX14. RnPEX14 complemented
peroxisomal import of catalase in ZP110 (Fig. 1c). Human
(HsPEX14) and Chinese hamster (ClPEX14) cDNA
were also cloned by colony hybridization from human and Chinese hamster
cDNA libraries, using RnPEX14 as probe. Both
HsPEX14 and ClPEX14 encoded 377-amino acid
Pex14p, with 96 and 94% identity to rat Pex14p at a deduced amino acid sequence level, while rat Pex14p was shorter by one amino acid, at
alignment position 329 (Fig. 2). Pex14p apparently contained at least
one hydrophobic segment and a coiled-coil region (Fig. 2,
underline and broken overline).
INTRODUCTION
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
-oxidation of very long chain fatty acids; biosynthesis of
plasmalogens, structural ether lipids abundant in the central nervous
system; interconversion of cholesterol to bile acids; and glyoxylate
transamination (1). The functional significance of human peroxisomes is
implicated by fatal genetic diseases such as Zellweger syndrome, linked
to peroxisomal malfunction and failure of peroxisome biogenesis (2, 3).
Genetic heterogeneity has been seen in subjects with these
peroxisome-deficient disorders, comprising 12 different complementation
groups (CGs)1 (4-7).
Peroxisomal proteins, including membrane proteins, are encoded by
nuclear genes, translated on free polyribosomes in the cytosol, and
posttranslationally translocated to preexisting peroxisomes (8).
Genetic analyses of peroxisome-deficient mutants of yeast and mammalian
cells have led to identification of a number of protein factors
essential for peroxisome biogenesis (3, 9). Cell fusion studies on
peroxisome-deficient Chinese hamster ovary (CHO) mutant cell lines (4,
7, 10-14) and fibroblasts from patients with peroxisomal biogenesis
disorders identified 13 CGs (4-7), including rhizomelic
chondrodysplasia punctata manifesting the defect solely in
peroxisome-targeting signal type 2 (PTS2) protein import. Furthermore,
two novel CGs, CG14 and CG15, were identified by newly isolated CHO
mutants, ZP110 and ZP114, respectively (15). Thereby, more than 15 genes are likely to be involved in mammalian peroxisome biogenesis. We
delineated mammalian PEX1 (16), PEX2 (4, 17),
PEX5 (14), PEX6 (18, 19), and PEX12
(20, 21), using peroxisome-deficient CHO cell mutants, demonstrating
that peroxisome assembly-defective CHO cell mutants are a useful system
to study peroxisome biogenesis and to elucidate primary defects of
human peroxisomal biogenesis disorders.
EXPERIMENTAL PROCEDURES
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
) (Stratagene). Nucleotide
sequence of both strands was determined by the dideoxy chain
termination method using Dye-terminator DNA sequence kit (Applied
Biosystems, Foster City, CA). Alignment was done using a CLUSTAL W
program (EMBL, Heidelberg, Germany).
) (Stratagene) at
SalI and NotI sites.
16
to 1,315, taking A of the initiation codon as 1) of the original
RnPEX14 cDNA clone, pBK-CMV·PAF-4, to the
pUcD2SR
MCSHyg vector (20, 21). CHO cell mutant ZP110 was transfected
with pUcD2Hyg·RnPEX14, followed by selection in the
presence of 200 µg/ml hygromycin B (Sigma). The hygromycin
B-resistant colonies formed on the coverslips were examined for
complementation of peroxisomes, 4 days after transfection.
Peroxisome-restored colonies were counted. Seven other groups of CHO
cell mutants, Z24, Z65, ZP92, ZP105, ZP109, ZP114, and ZP119, and
fibroblasts derived from peroxisome-deficient patients were
similarly transfected with pUcD2Hyg·RnPEX14. A stable cell
clone of the RnPEX14 transformant of ZP110 was isolated as follows. Three of five hygromycin B-resistant transformant clones were
peroxisome-positive, on the basis of immunostaining; one of the three,
named 110P14, was cloned by the limiting dilution method. Transfection
with human and Chinese hamster PEX14 (HsPEX14 and
ClPEX14, respectively) was likewise done, as with
RnPEX14.
) and sequenced as described (21). ZP161-derived PEX14 cDNA was cloned into pUcD2SR
MCSHyg and
transfected to CHO cells by lipofection.
) (pBS·RnPEX14-myc) by replacing the
NheI-KpnI fragment of pBS·RnPEX14
with NheI-KpnI fragment of PCR-amplified
products using the primers described above. Plasmid
pUcD2Hyg·RnPEX14-myc was constructed by inserting the
XbaI-KpnI fragment of
pBS·RnPEX14-myc into pUcD2SR
MCSHyg vector (21).
Flag-tagging to the N terminus of RnPEX14 protein was
likewise done by PCR using a forward primer,
5'-GCCCAGATCTTGGCGTCCTCGGAGC, and a reverse primer,
5'-TCAGGGCTGTGCCCGTCCTT-3'. Flag-tagged RnPEX14 in
pUcD2Hyg·SR
vector (pUcD2Hyg·flag-RnPEX14) was
constructed as follows. The BamHI fragment of
pUcD2Hyg·RnPEX12 (21) was replaced with a
BglII-BamHI fragment of the PCR products; the plasmid was then digested with ApaI and ligated with the
ApaI fragment of pBS·RnPEX14. All plasmid
constructs were assessed by nucleotide sequence analysis.
Pex14p-myc and Flag-Pex14p were detected using mouse
monoclonal antibodies to human c-myc (9E10) (Santa Cruz
Biotechnology, Inc., Santa Cruz, CA) and Flag (M2) (Scientific Imaging
Systems, New Haven, CT), and Texas Red-labeled sheep anti-mouse IgG
second antibody (Amersham Pharmacia Biotech, Tokyo, Japan), in cells
that had been permeabilized with either 25 µg/ml of digitonin or 1%
Triton X-100.
-glucosaminidase for lysosomes were assayed, essentially as described (17). Protein in each fraction
was separated by SDS-PAGE, and electrophoretically transferred onto
polyvinylidene difluoride membrane (Bio-Rad). Pex14p and marker
proteins were probed with respective antibodies and then visualized
with ECL Western blotting detection reagent (Amersham Pharmacia
Biotech). For determination of intraperoxisomal localization, peroxisomal fraction was diluted with 20 mM Hepes-KOH, pH
7.6, and sonicated four cycles for 30 s each in the presence of 1 M NaCl (17). Membrane and soluble fractions were separated
by centrifugation for 30 min at 100,000 × g. Sodium
carbonate treatment (26) and Triton X-114 extraction (27) were
performed as described.
-D-thiogalactoside was added to the final
concentration of 1 mM. After 2 h at 37 °C, the
cells were harvested, resuspended in 1 ml of chilled PBS containing 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, and
then sonicated. Lysates were centrifuged, and supernatant was
collected. The supernatant was incubated with 100 µl of 50% slurry
glutathione-Sepharose (Amersham Pharmacia Biotech) at 4 °C for
1 h. Glutathione-Sepharose beads were washed three times and
resuspended in 250 µl of binding assay buffer: 50 mM
HEPES-KOH, pH 7.4, 150 mM NaCl, 0.2% Nonidet P-40, 10%
glycerol, 0.25% BSA, 25 µg/ml each of leupeptin and antipain, 50 units/ml aprotinin, 1 mM phenylmethylsulfonyl fluoride, 1 mM EDTA, and 1 mM dithiothreitol. GST-Pex5pS
and GST-Pex5pL were separately mixed with Glutathione-Sepharose beads
at 4 °C. After a thorough wash, the protein-bound Sepharose beads
were suspended in 10 volumes of a cleavage buffer, 50 mM
Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol, 0.1% Nonidet P-40. GST-Pex5p was
cleaved with PreScission Protease (Amersham Pharmacia Biotech) at
4 °C for 5 h, and Pex5p was isolated as an eluate according to
the procedure recommended by a manufacturer.
-32P]dCTP (Amersham Pharmacia Biotech). Western blot
analysis was done with primary antibodies, including anti-Pex12p
antiserum (20, 21), and a second antibody, donkey anti-rabbit IgG
antibody conjugated to horseradish peroxidase (Amersham Pharmacia
Biotech), using ECL Western blotting detection reagent (Amersham
Pharmacia Biotech). 12-(1'-pyrene)dodecanoic acid/long wavelength
ultraviolet light (P12/UV) and 9-(1'-pyrene)nonanol/UV (P9OH/UV)
resistance was determined under conditions of 2 µM/1.5
min and 6 µM/2 min (4), respectively.
RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
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Fig. 1.
Restoration of peroxisomes in CG14 CHO mutant
cells. a and e, peroxisome-deficient mutant ZP110
cells; b, peroxisome-restored ZP110, after lipofection with a combined
pool (A9) of rat cDNA library. The arrows indicate the
complemented cells. Cytosolic appearance of catalase was apparent in
the other cells. c, ZP110 transfected with
pUcD2Hyg·RnPEX14 plasmid. d and f,
110P14 cells, stable RnPEX14-transformants of ZP110 cells.
Cells were stained with antisera to catalase (a-c), PTS1
peptide (d), and PMP70 (e and f),
respectively. Magnification, × 630; bar, 20 µm.
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Fig. 2.
Amino acid sequence alignment of
PEX14 protein from three mammalian species and yeast
Pex14p from S. cerevisiae and H. polymorpha. Deduced amino acid sequence of rat
(Rn) PEX14 was compared with those of Pex14p from
human (HsPEX14), Chinese hamster (ClPEX14),
S. cerevisiae (ScPEX14) and H. polymorpha (HpPEX14). Hyphens represent
spaces. Identical amino acids between species, including two or more
mammalian ones, are shaded. A putative membrane-spanning
segment is underlined; the dashed
overline indicates a predicted coiled-coil region, where
conserved hydrophobic amino acids are marked by dots. The
sequence used for chemical synthesis of Pex14p peptide is shown by a
dashed underline. The solid
arrowheads indicate the position of mutation in ZP161 (see
Fig. 4). The GenBankTM data base accession numbers for rat,
human, and Chinese hamster PEX14 genes are AB017544,
AB017546, and AB017545, respectively.
PEX14 Restored Peroxisome Biogenesis in ZP110
Several phenotypic abnormalities due to peroxisome deficiency, such as impaired import of PTS1 and PTS2 proteins, were found in ZP110 (15). To determine whether RnPEX14 could correct these phenotypes, a stable RnPEX14 transformant of ZP110, named 110P14, was isolated. PTS1 proteins were noted in numerous vesicular structures, presumably peroxisomes, when stained with antibodies to PTS1 (Fig. 1d), catalase and 3-ketoacyl-CoA thiolase (data not shown). Numerous PMP70-positive particles were detected in 110P14 cells (Fig. 1f), whereas peroxisomal remnants, larger PMP70-positive vesicles but fewer in number, were discernible in ZP110 (15) (Fig. 1e). These results strongly suggested that 110P14 cells had morphologically normal peroxisomes, as seen in the wild-type CHO-K1 cells. When other CHO cell mutants, ZP111 (15) and ZP161 (7), of the same CG as ZP110, were transfected with human PEX14 (HsPEX14), PTS1 proteins were likewise localized in peroxisomes, demonstrating HsPEX14 to be functional in CHO cells (see Table II).
In peroxisome-deficient cells, peroxisomal proteins are mislocalized to
the cytosol, rapidly degraded, or not converted to mature forms,
despite normal synthesis (4, 11, 13). In the digitonin titration assay,
nearly 60% of the catalase activity was latent at the digitonin
concentration of 100 µg/ml in the wild-type cells (Fig.
3A). In ZP110 cells, full
activity of catalase was detected at 100 µg/ml digitonin, with the
same latency profile as lactate dehydrogenase, a cytosolic enzyme;
hence, catalase was present in the cytosol (Fig. 3A). This
was consistent with our earlier observation (4, 11, 16, 21). In 110P14
cells, catalase showed almost the same latency as in the wild-type
CHO-K1 cells, demonstrating restoration of peroxisome biogenesis.
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AOx, the first enzyme of the peroxisomal fatty acid -oxidation
system, is synthesized as a 75-kDa polypeptide (A component) and is
proteolytically converted into 53- and 22-kDa polypeptides (B and C
components, respectively) in peroxisomes (11, 30, 34). All three
polypeptide components were evident in CHO-K1 cells, upon continuous
cell labeling with [35S]methionine and
[35S]cysteine followed by immunoprecipitation (Fig.
3B, lane 1), but only the A component
was seen in the ZP110, in a much smaller amount, probably due to rapid
degradation (11) (Fig. 3B, lane 2).
The three components of AOx were found in 110P14, as in CHO-K1 cells
(Fig. 3B, lane 3), indicative of
proper import and proteolytic conversion of AOx. Peroxisomal
3-ketoacyl-CoA thiolase, the third enzyme of the peroxisomal
-oxidation system, is synthesized as a larger precursor with an
amino-terminal presequence that contains PTS2 (35, 36) and is converted
to a mature form in peroxisomes (11, 29, 37, 38). In wild-type CHO-K1
cells, only the matured thiolase was detected (Fig. 3B,
lane 4), thereby reflecting rapid processing of
the precursor form. In ZP110 cells, only the larger precursor was found
(lane 5), implying the absence of processing activity. 110P14 cells showed only the mature form of thiolase, demonstrating the complementation of PTS2 protein import and processing (Fig. 3B, lane 6). Proper processing
of AOx and thiolase was also evident, as assessed by immunoblotting, in
161P14, i.e. ZP161 cells stably transfected with
RnPEX14, confirming the restored biogenesis of peroxisomes
(Fig. 3C). These results demonstrated that
RnPEX14 restored peroxisome biogenesis in ZP110 and ZP161.
Treatment with P12/UV specifically kills peroxisome-deficient mutants, due to almost no synthesis of plasmalogen (4, 39), whereas P9OH/UV selection kills wild-type cells, which incorporate this fatty alcohol analogue into plasmalogen molecules (4, 40). 110P14 cells showed the resistance to P12/UV treatment and P9OH/UV-sensitive phenotype, as was the case for CHO-K1 cells (Table I). Conversely, ZP110 was sensitive to the P12/UV but resistant to the P9OH/UV, consistent with our earlier observation (15). Taken together, RnPEX14 could fully complement the abnormality of CG14 ZP110.
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At the 4th day after RnPEX14 transfection, CG14 CHO mutants, ZP110, ZP111, and ZP161, all were mostly complemented for peroxisome assembly, whereas none of seven other CGs of peroxisome-deficient CHO cell mutants, ZP92, Z24/ZP107, Z65, ZP105, ZP109, ZP114, and ZP119, showed peroxisomes (Table II). Furthermore, RnPEX14 was introduced into fibroblasts from patients with peroxisomal biogenesis disorders, such as Zellweger syndrome, of CGs A, B, D, and G of Gifu University (Gifu, Japan) (15) and CG-VI of the Kennedy-Krieger Institute (Baltimore, MD) (15) that were distinct from CHO mutants. As expected, none of the peroxisomal biogenesis disorder fibroblasts were morphologically restored for peroxisome assembly (Table II). These results, together with those described above, demonstrate that Pex14p is a peroxisome biogenesis factor only for CG14.
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Expression of PEX14
Peroxisomes and peroxisomal proteins are induced in rat liver by
administration of hypolipidemic agents, such as clofibrate (41, 42).
RnPEX14 mRNA was not induced by clofibrate, as assessed by Northern blotting (Fig. 4A,
lanes 1 and 2, upper
panel), under which AOx mRNA was induced by about 50-fold
(middle panel), findings consistent with previous
observations (21, 42, 43). PEX14 mRNA was detected as a
single band of ~2 kilobase pairs in CHO-K1 and ZP161 (Fig.
4A, lanes 3 and 4). The
amount and size of PEX14 mRNA were indistinguishable
between the mutant and wild-type CHO cells, suggesting that alteration
in the PEX14 gene in ZP161 was not at a transcriptional
level. PEX14 mRNA in CHO cells was similar in size but
at much lower levels, as compared with those in rat liver (Fig.
4A; note that poly(A)+ RNA was loaded on
lanes 3-5). It is noteworthy that
PEX14 mRNA in ZP110 was nearly undetectable, implying
alteration at a transcriptional level or rather rapid degradation of
PEX14 mRNA (Fig. 4A, lane 5). The undetectable level of PEX14 mRNA in
ZP110 was confirmed by reverse transcription-PCR, using up to 2-5 µg
of poly(A)+ RNA (data not shown).
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Dysfunction of Pex14p in CHO Mutants
PEX14 in Mutants--
To investigate dysfunction of Pex14p in
ZP161, we determined the nucleotide sequence of Pex14p cDNA
isolated from ZP161 by reverse transcription-PCR. In 12 independent
cDNA clones isolated, six clones showed a 133-bp deletion from
nucleotide C at position 37 of a codon for Pro13 to G at
position 169 of a codon for Gly57, termed
ClPEX14del133, resulting in a frameshift that created a
mutation at Pro13 to Gly immediately followed by a
termination codon at amino acid alignment position 14 (Fig.
4B, ZP1611, upper panel). In six remaining
cDNA clones, a 103-bp deletion from nucleotide A at position 385 of
a codon for Lys129 to G at position 487 of a codon for
Val163 was likewise identified, termed
ClPEX14del133/103, in addition to the 133-bp deletion
described above, thereby resulting in another frameshift with a
termination codon at position 129 in the deduced sequence (Fig.
4B, ZP161
2, lower panel). Thus, dysfunction of the Pex14p caused by a deletion is most likely to be the primary defect
in the mutant ZP161. No reverse transcription-PCR product was obtained
from ZP110-derived mRNA, consistent with the finding by Northern
analysis (see Fig. 4A) (data not shown).
Complementation of Protein Transport by ClPEX14-- When mutant ZP161 cells were transfected with ClPEX14 cDNA from the wild-type CHO-K1, PTS1 proteins were found in punctate staining, thereby indicating complementation of peroxisomal protein import (Fig. 4C, a), as was the case by HsPEX14 (data not shown). Transfection of a mock vector did not alter intracellular location of PTS1 proteins in ZP161 (data not shown). To assess the impaired function of Pex14p in ZP161, two types of ZP161-derived PEX14 cDNAs, ClPEX14del133 with a 133-bp deletion and ClPEX14del133/103 with two-site, 133- and 103-bp deletions, were separately transfected back to the mutant cells. PTS1 proteins were present in the cytosol, in a diffuse manner, in both ZP110 transfected with ClPEX14del133 and ClPEX14del133/103-transfected ZP161 (Fig. 4C, b and c, respectively), hence demonstrating dysfunction of the mutated forms of Pex14p. Similar results were likewise obtained for ClPEX14del133-transfected ZP161 and ClPEX14del133/103-transfected ZP110 cells (data not shown). Import of catalase was not restored in these transfectants as assessed by cell staining with anti-catalase antibody (data not shown). Moreover, ZP111, another mutant of CG14 (15), showed cytosolic staining of PTS1 proteins in transfectants with ZP161-derived PEX14 (data not shown), confirming the impaired function of ClPEX14del133 and ClPEX14del133/103.
Collectively, we conclude that dysfunction of Pex14p caused by truncation is the primary defect in impaired peroxisome biogenesis in CG14 CHO mutant ZP161.
Intracellular Localization of Pex14p
The C-terminal peptide of rat Pex14p (residues 357-376) was used
to raise rabbit antibody. This antibody reacted with a protein of an
apparent mass of ~56 kDa, larger than the predicted size, in
immunoblot of rat liver homogenates (Fig.
5A, lane
4). The mobility of Pex14p in SDS-PAGE was indistinguishable
from that of Pex14p synthesized in vitro by coupled
transcription/translation of RnPEX14 that was specifically
immunoprecipitated by anti-Pex14p antibody but not by preimmune serum
(lanes 1-3), thereby indicating that a cloned
RnPEX14 encodes bona fide Pex14p. This result implies the
synthesis of Pex14p at its final size, consistent with a general rule
for peroxisomal proteins (8) (see below). The reason for the reduced
mobility of Pex14p in SDS-PAGE is presently unknown.
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Intracellular localization of Pex14p was investigated by subcellular
fractionation of rat liver. Pex14p was detected in a postnuclear
supernatant fraction and then exclusively present in the organellar
fraction, not in cytosol (Fig. 5A, lanes
5-7). Pex14p was apparently enriched in isolated
peroxisomes (lane 8). Upon further fractionation
of the light mitochondrial fraction by sucrose density gradient
centrifugation, Pex14p was detected as a single band and co-sedimented
with a peroxisomal marker enzyme, catalase, and AOx as well as
peroxisomal integral membrane proteins, such as Pex12p, although less
in amount (20, 21) and PMP70 (data not shown), thereby indicating that
Pex14p is a peroxisomal protein (Fig. 5B). This was
consistent with morphological observation (see below). The apparently
slight difference in the distribution of Pex12p, as compared with that
of Pex14p and AOx, is presently unknown. The distribution of Pex14p on
the gradient was distinct from those of the marker enzymes, glutamate
dehydrogenase for mitochondria, esterase for microsomes, and
N-acetyl--glucosaminidase for lysosomes, confirming the peroxisomal
location of Pex14p.
The subcellular localization of Pex14p was also determined by
immunofluorescent microscopy using Pex14p tagged at its N terminus with
an epitope Flag. In the wild-type CHO-K1 cells transfected with
Flag-RnPEX14, Pex14p was detected in a punctate staining pattern with anti-Flag antibody (Fig.
6A, a). The pattern
was superimposable on that obtained using anti-PTS1 antibody, thereby demonstrating that Flag-Pex14p was targeted to peroxisomes (Fig. 6A, b and c). Similar results were
obtained when RnPEX14 was expressed in CHO-K1 cells and
stained using anti-Pex14p peptide antibody (data not shown). The
flag-Pex14p fully restored peroxisome assembly in ZP110, as efficiently
as Pex14p and was colocalized with PTS1 protein, indicating that the
N-terminal tagging did not interfere with Pex14p function (Fig.
6A, d and e). These results were
interpreted to mean that Flag-Pex14p and Pex14p were translocated to
peroxisomes. Flag-Pex14p was colocalized with PMP70 in peroxisomal
ghosts in other complementation groups of CHO cell mutants, such as
PEX2-defective Z65 (17) (Fig. 6A, f
and g), as was the case for Pex12p (21). Thus, translocation
of Pex14p does not appear to be impaired in these mutant cells. It is
noteworthy that endogenous Pex14p was also detectable by anti-Pex14p
antibody, in CHO-K1 and PEX6-deficient ZP92 (4, 18) (Fig.
6A, h and i). Collectively, the data
demonstrate peroxisomal localization of Pex14p.
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Hydropathy analysis of Pex14p suggested that Pex14p contains at least one hydrophobic segment (see Fig. 2, solid line). Pex14p was not extracted with 50 mM Hepes-KOH (pH 7.6), from freshly isolated rat liver peroxisomes (data not shown). The integrity of Pex14p was verified by sonication in 50 mM Hepes-KOH (pH 7.6), 1 M NaCl; treatment with 1% Triton X-114 (27); and extraction with 0.1 M sodium carbonate (pH 11.5) (26, 44) (Fig. 6B). Pex14p was not extractable with either 1 M NaCl or sodium carbonate, as was the case for peroxisomal integral membrane proteins, PMP70 (25) and Pex12p (20, 21), but in contrast to a matrix enzyme, AOx, thereby strongly suggesting that Pex14p is an integral membrane protein (Fig. 6B, lanes 2, 3, 6, and 7). Upon treatment with Triton X-114, Pex14p, but not PMP70 and Pex12p, was recovered more in an aqueous phase than in a detergent phase (lanes 4 and 5), probably due to the presence of a highly charged region at the N-terminal part of Pex14p. A similar observation for highly charged membrane glycoproteins was reported by Bodier (27).
Membrane Topology of Pex14p
Topology of Pex14p in peroxisomal membranes was investigated by
the differential cell permeabilization procedure, where detection of
Pex14p was performed using anti-epitope tag antibody. When the
wild-type CHO-K1 cells transfected with RnPEX14-myc encoding C-terminally myc-tagged Pex14p were permeabilized with 25 µg/ml of digitonin, under which plasma membranes are selectively
permeabilized and intraperoxisomal proteins are inaccessible to
exogenous antibodies (20, 21). Pex14p-myc was observed in a
punctate staining pattern, whereas almost no staining of cells was
noted with anti-PTS1 antibody (Fig.
7A, a and
b). When the Pex14p-myc-expressing CHO-K1 cells were treated with Triton X-100, which solubilizes all cellular membranes, both Pex14p-myc and PTS1 proteins were detected
in particulates similar in number, presumably peroxisomes (Fig.
7A, c and d). The data demonstrating
exactly the same topology were also obtained with a set of antibodies
to Pex14p C-terminal peptide and catalase (not shown). Similar results
were likewise obtained in studies of RnPEX14-myc-transfected
ZP110 cells (data not shown). CHO-K1 cells expressing
Pex14p-myc were pemeabilized as above and then incubated
with Proteinase K. In an immunoblot of mock-treated cells,
Pex14p-myc was detected using anti-myc antibody,
where AOx was used as a matrix protein marker (Fig. 7B,
lane 1). Upon cell permeabilization with
digitonin, Pex14p-myc became sensitive to Proteinase K and
was no more detectable, while AOx was not accessible to the protease
(lane 2). Both Pex14p and AOx were no more
discernible after protease treatment in the presence of Triton X-100
(lane 3). The data together strongly suggest that the C-terminal part of Pex14p-myc is exposed to the
cytosol.
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To determine the membrane topology of N-terminal part of Pex14p, N-terminally Flag-tagged Pex14p was expressed in CHO-K1 cells. Flag-Pex14p was detected in an immunoblot, using antibodies to Flag (Fig. 7B, lanes 1) and Pex14p C-terminal peptide (data not shown). After cell permeabilization using digitonin, Flag-Pex14p became accessible to Proteinase K digestion (Fig. 7B, lane 2), as was the case for Pex14p-myc, thereby indicating the Flag N-terminal part of Pex14p to be facing the cytosol. This conclusion was confirmed by using anti-Pex14p C-terminal peptide antibody (data not shown). It is noteworthy that Flag-RnPEX14-transfected CHO-K1 was not readily stained using anti-Flag antibody (data not shown). It is plausible that the Flag epitope was inaccessible to the antibody, presumably due to steric hindrance by other peroxisomal proteins.
Taken together, the results demonstrate that N- and C-terminal parts of Pex14p are exposed to the cytosol.
Expression and Purification of Fusion Proteins
To investigate whether Pex14p interacts with other peroxins such as a PTS2 receptor Pex7p and Pex5p, a PTS1 receptor, fusion protein of Pex14p with GST, GST-Pex5pS, GST-Pex5pL, and His-tagged human Pex7p were expressed in E. coli. Each protein was purified by affinity chromatography of E. coli lysates using their respective ligand columns. Pex5pS and Pex5pL were isolated from the GST fusion protein. The purity of all proteins was assessed by SDS-PAGE.
Pex14p Interacts with Pex5p and Pex7p
In an in vitro Pex14p-binding assay,
35S-labeled 3-ketoacyl-CoA thiolase, a PTS2 protein (35,
38), was detected at 20% recovery of the input, as determined by
quantitation of the autoradiogram, by incubation with GST-Pex14p plus
His-Pex7p (Fig. 8A,
lane 5), while 35S-labeled thiolase
was found at a nearly background level with His-Pex7p plus GST,
GST-Pex14p, or GST (lanes 2-4), thereby
demonstrating Pex7p-mediated interaction of Pex14p with PTS2 protein to
be specific (see also Fig. 8A, lower panel).
35S-labeled AOx, a PTS1 protein (24, 30), was found in an
unbound fraction in the assay, thus indicating Pex7p to be specific for PTS2 (data not shown). Moreover, 35S-labeled Pex7p was
recovered by incubation with GST-Pex14p, indicating direct binding of
Pex7p to Pex14p (data not shown).
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We identified two isoforms of Pex5p, Pex5pS and Pex5pL, that restored peroxisome biogenesis in CG-II CHO cell mutants, ZP105 and ZP139 (14). Pex5pL is involved in peroxisomal import of not only PTS1 proteins but also PTS2 proteins, while Pex5pS apparently transports only PTS1 proteins (14, 45). Both Pex5pS and Pex5pL bound to GST-Pex14p, as assessed by immunoblot, together with 35S-AOx in the Pex14p-binding assay (Fig. 8B, lanes 3 and 4). GST-Pex14p showed almost no direct binding to 35S-AOx (lane 2), while both GST-Pex5pS and GST-Pex5pL bound to 35S-AOx at a similar level (data not shown), thereby implying that both Pex5p isoforms mediate the interaction of Pex14p with PTS1. Specific Pex5p-mediated PTS1 protein binding to Pex14p was envisaged by quantitation of 35S-AOx, where nearly 40% of the input AOx was recovered in the case of both Pex14p/Pex5pS and Pex14p/Pex5pL, evidently indicative of a complex comprising these three components (Fig. 8B, lower panel). In contrast, no binding of 35S-labeled thiolase was observed in this assay, confirming Pex5p to specifically recognize PTS1, not PTS2 (data not shown). Similar results showing interaction of Pex14p with Pex5p/35S-AOx were obtained using His-tagged protein such as His-Pex5p (data not shown). Moreover, 35S-labeled Pex5pS and Pex5pL bound to GST-Pex14p (data not shown), confirming the direct interaction of Pex14p with Pex5p.
Taken together, Pex14p was evidently demonstrated to interact with Pex7p/PTS2 protein and Pex5p/PTS1 protein, thereby implying Pex14p-mediated import of PTS1 and PTS2 proteins into peroxisomes.
Binding to Pex14p
To determine whether Pex14p interacts with itself, GST-Pex14p was
incubated with 35S-labeled Pex14p. 35S-Pex14p
was recovered with GST-Pex14p at a nearly 4-fold amount as compared
with the amount with GST, suggesting that Pex14p proteins bind each
other in a homomeric manner (Fig. 9,
A and B). The forms of homomeric interaction,
i.e. dimer or oligomer, are presently unknown.
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DISCUSSION |
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In the present work, we isolated rat PEX14 cDNA encoding 41-kDa integral membrane protein of peroxisomes, by functional complementation of peroxisome-deficient CHO cell mutant, ZP110. CG14 mutants, ZP110, ZP111, and ZP161, were restored in peroxisome assembly, for both morphological and biochemical criteria. None of the fibroblasts from patients with peroxisome deficiency disorders of 11 CGs was complemented, indicating that the PEX14 gene is not the causal gene of any CG of human peroxisome-deficient disorders thus far classified. PEX14 is novel; the eighth gene required for peroxisome assembly in mammals, including humans; and responsible for the defects in peroxisome assembly of CG14. We have shown that a cloning strategy using mammalian expression vector is highly efficient in delineating peroxisome biogenesis factors in mammals, i.e. PEX1 (16), PEX2 (17, 22), PEX5 (14), PEX6 (18, 19), and PEX12 (20, 21), including the present work. While several human orthologues of yeast genes have recently been cloned by expressed sequence tag search, such as PEX1 (46, 47), PEX5 (48), PEX7 (28, 49, 50), PEX12 (51), and more recently identified PEX10 (52, 53) and PEX16 (54), direct cDNA cloning by functional complementation is an independent, parallel approach that often complements the cloning of human cDNAs of interest using expressed sequence tags. It is noteworthy that Fransen et al. (55) very recently identified Pex14p, using a combination of a peroxisomal protein import system in semipermeabilized CHO cells and antisera to peroxisomal membrane proteins. Human Pex14p was shown to bind Pex5pS in ligand blots, in good agreement with our findings.
The peroxin Pex14p from three mammalian species (i.e. rat, human, and Chinese hamster, each comprising 376, 377, and 377 amino acids) all showed nearly 30% identity in the amino acid sequence to Pex14p of H. polymorpha (31) and S. cerevisiae PEX14 (32, 33), indicating a high degree of conservation of Pex14p in eukaryotic evolution. At present, however, we do not know if the yeast PEX14 gene can complement ZP110 and the mammalian PEX14 restores peroxisomal protein import in the yeast pex14 mutants (31-33). This type of complementation may be less likely, since no direct complementation by yeast genes of mammalian peroxisome assembly-defective mutant cells, including human patient fibroblasts, has been reported to date (3, 7). In the present study, dysfunction of Pex14p in ZP161 cells was evidently demonstrated to be due to truncation in PEX14 gene transcripts. Furthermore, both alleles of PEX14 were found to be expressed in CHO cells, at least in ZP161, although the type of gene expression in CHO cells has been postulated to be hemizygotic. The level of PEX14 mRNA derived from each allele remains to be determined.
The present finding that Pex14p is located in the peroxisomal membranes as an integral PMP, with topology of the N- and C-terminal parts exposed to the cytosol, agrees well with the observation by Wendland and Subramani (56) demonstrating in patient fibroblasts that the molecular defects are mostly associated with the organelles, while cytoplasmic factors seem to be functionally normal. It is noteworthy that S. cerevisiae Pex14p has been shown to be peripheral (32) or integral (33) membrane protein of peroxisomes, H. polymorpha Pex14p being integral (31). While peroxisomal transport of membrane proteins, including PMP70, appears to be normal in the CG14 mutants, ZP110, ZP111, and ZP161, import of soluble proteins such as PTS1 and PTS2 proteins is defective. We showed that Pex14p interacts with a WD motif protein Pex7p (PTS2 receptor) and PTS2 protein, as was the case for S. cerevisiae Pex14p (32, 33). Pex14p likewise binds to a tetratricopeptide repeat (TPR) protein Pex5p (PTS1 receptor), both isoforms Pex5pS and Pex5pL (14), carrying their cargo PTS1 protein. This is compatible with the finding in S. cerevisiae by in vivo studies using the yeast two-hybrid system (32, 33), although only one type of Pex5p is present in yeast.
Given the fact that Pex5p and Pex7p bind to PTS1 and PTS2 proteins,
respectively, it is more likely that several types of the
receptor-cargo complex, such as Pex5p-PTS1 protein and Pex7p-PTS2 protein, translocate to a peroxisomal import machinery, presumably comprising Pex14p as well as other peroxin(s) and mediating the transport of polypeptides into the peroxisomal matrix. Thereby, Pex14p
possibly plays a pivotal role in the peroxisome assembly process as a
factor, e.g. a convergent component of the import machinery
of matrix proteins but not membrane polypeptides. The interacting
site(s) of Pex14p with Pex5p and Pex7p, both carrying their cargoes,
are presumably in the cytosolic phase of Pex14p. Precise domains of
Pex14p, each responsible for the interaction with Pex5p and Pex7p as
well as for the homomeric binding, remain to be investigated. It would
be also intriguing to study whether Pex14p interacts with other
mammalian peroxisome biogenesis factors so far identified, including
RING peroxins Pex2p (17, 22, 43), Pex10p (52, 53), and Pex12p (20, 21,
51); AAA family peroxins Pex1p (16, 46, 47) and Pex6p (18, 19, 57); a
Src homology 3 peroxisomal membrane protein Pex13p (58); and Pex16p
(54). Such studies may be done by in vitro binding assay
developed in this report and using semipermeabilized CHO cells,
including wild-type CHO-K1 as well as PEX14 mutants ZP110, ZP111, and ZP161.
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ACKNOWLEDGEMENTS |
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We thank N. Shimozawa and H. Moser for patient fibroblasts of complementation groups A, B, D, and G and group VI, respectively. We also thank T. Tsukamoto, N. Shimozawa, and T. Osumi for comments and K. Mizuno and other members of the Fujiki laboratory for discussion.
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FOOTNOTES |
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* This work was supported in part by a CREST grant (to Y.F.) from the Japan Science and Technology Corporation and Grants-in-Aid for Scientific Research 07408016, 08249232, 08557011, 09044094, 09258215, and 10129224 (to Y. F.) from the Ministry of Education, Science, Sports and Culture.
¶ To whom correspondence should be addressed: Dept. of Biology, Kyushu University Faculty of Science, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Tel.: 81-92-642-2635; Fax: 81-92-642-4214 or 81-92-642-2645; E-mail: yfujiscb{at}mbox.nc.kyushu-u.ac.jp.
2 H. Otera, and Y. Fujiki, unpublished observations.
3 S. Mukai, and Y. Fujiki, unpublished observations.
4 T. Harano, and Y. Fujiki, unpublished observations.
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ABBREVIATIONS |
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The abbreviations used are: CG, complementation group; AOx, acyl-CoA oxidase; CHO, Chinese hamster ovary; GST, glutathione S-transferase; Pex5pS and Pex5pL, shorter and a longer isoforms of Pex5p; PEX14, cDNA encoding the peroxin Pex14p; PMP70, 70-kDa peroxisomal integral membrane protein; P9OH/UV, 9-(1'-pyrene)nonanol/ultraviolet; P12, 12-(1'-pyrene)dodecanoic acid; PTS1 and PTS2, peroxisome targeting signal types 1 and 2; CMV, cytomegalovirus; PAGE, polyacrylamide gel electrophoresis; bp, base pair(s).
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REFERENCES |
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