From the Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama 226-8501, Japan
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ABSTRACT |
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5,6-Dichloro-1- The nucleoside analog
DRB1 is a classic inhibitor
of transcription elongation by pol II (reviewed in Ref. 1). Although it has been used for more than three decades, its mode of action has long
been a mystery. DRB is unique in that it shows no effect on
transcription reconstituted with purified general transcription factors
(GTFs) and pol II, whereas it potently represses transcription in
cruder systems or in vivo (2-4). Therefore, one or more
factors apart from GTFs and pol II appear to be involved in
DRB-sensitive transcription (1). Such putative factors must play
general roles in pol II transcription in vivo because DRB
affects most of the class II genes (4-6).
Recently, we and others have identified two elongation factors
essential for DRB-sensitive transcription. One of them, positive transcription elongation factor b (P-TEFb), is a protein kinase that
phosphorylates the pol II C-terminal domain (CTD) in a DRB-sensitive fashion (7-12). The CTD phosphorylation likely plays a pivotal role in
pol II elongation and may be relevant to the P-TEFb function. The
other, DSIF, has been purified from HeLa cell nuclear extract, based on
its ability to induce DRB-sensitivity in vitro (3). DSIF is
composed of 160- and 14-kDa subunits, which are homologs of the
Saccharomyces cerevisiae transcription factors Spt5 and Spt4
(3, 13-15). DSIF/Spt4-Spt5 genetically and physically interacts with
pol II, and thus may directly regulate pol II processivity (3, 16).
The function of DSIF in the absence of DRB remains obscure. Small
amounts of DSIF, when added back to a DSIF-depleted transcription system, repress transcription only in the presence of DRB, without affecting transcription in its absence (3). Higher doses of DSIF,
however, repress transcription even in the absence of DRB (3). In
contrast, under limiting concentrations of NTPs, DSIF stimulates
transcription elongation in the absence of DRB (3). Genetic analysis in
yeast also suggests a stimulatory role for Spt4-Spt5 under low NTP
concentrations in vivo (16).
DSIF p160 has many distinctive structural features (Refs. 1 and 3; see
Fig. 1A). The N-terminal region is highly acidic, and
several hexapeptide repeats are found at the C terminus. In the central
domain, there are four stretches with significant similarity to NusG, a
prokaryotic transcription termination and anti-termination factor
(17-19). The corresponding region of NusG has recently been found to
share homology with a class of proteins involved in translation and is
termed the KOW motif, though its function is unknown (20). We have
named the four stretches KOW1 to 4, respectively (see Fig.
1A).
In this report, we have characterized the structure and function of
DSIF p160. We show that p160 is a nuclear protein that forms a stable
complex with p14 and interacts with the pol II largest subunit. In
addition, we map the regions of p160 that are involved in interactions
with p14 and pol II and those that are essential for DSIF activity.
Finally, we isolate a p160 mutant that acts in a dominant-negative
manner and use this mutant to present evidence that DSIF is a negative
regulator of transcription in vivo.
Plasmid Constructs--
For expression of recombinant proteins,
the following plasmids were constructed in the expression vector
pET-14b (Novagen) by subcloning from parent vectors described elsewhere
(3): pET-p160
For expression of p160 Preparation of Recombinant (r-) p160 and r-p14
Proteins--
Histidine-tagged p160 and p14 were expressed in
Escherichia coli strain BL21 (DE3) transformed with
pET-DSIFp160 or pET-DSIFp14 (3). Cells were harvested and lysed by
sonication, the lysates were cleared by centrifugation and filtration,
and r-p160 and r-p14 were purified from the supernatants by Ni-affinity
column chromatography according to the instructions of the manufacturer (Novagen). To further purify the recombinant proteins, the purified fractions were subjected to SDS-PAGE, and the full-length proteins were
recovered from a gel slice. The proteins were then precipitated with
acetone, denatured with 6 M guanidine-HCl, and
renatured by dialysis against 10 mM HEPES (pH 7.9), 10%
glycerol, 50 mM KCl, 0.2 mM EDTA, 0.5 mM dithiothreitol as described (23).
To prepare in vitro translated p160 and its derivatives,
either pBS-p160, pBS-p160Nterm, or
pBS-p160Cterm was digested with appropriate restriction
enzymes, some of which cleave the p160 open reading frame to produce
the C-terminally truncated products. The linearized plasmids were then
transcribed with either T7 or T3 RNA polymerase, followed by
translation using a rabbit reticulocyte lysate system (Promega) in the
presence of [35S]methionine.
p160-p14 Interaction Analysis--
pCMV-FLAGp160 (10 µg) and
pCMV-HAp14 (10 µg) were transfected into 2 × 106
HeLa cells either individually or in combination. Forty-eight hours
post-transfection, the cells were lysed in 500 µl of high salt buffer
(50 mM Tris (pH 7.9), 500 mM NaCl, 1% Nonidet
P-40) and cleared by centrifugation. The lysates were incubated with 2 µg of anti-Flag M2 monoclonal antibody (Sigma) for 2 h at
4 °C and then with 20 µl of protein G-Sepharose (Amersham
Pharmacia Biotech) for 1 h at 4 °C. After washing three times
with high salt buffer, the immunoprecipitates were eluted with 50 µl
of SDS sample buffer and analyzed by immunoblotting. Flag-p160 and HA-p14 were probed with anti-Flag and anti-HA (12CA5) monoclonal antibody (Life Technologies, Inc.), respectively, and were visualized using an ECL kit (Amersham Pharmacia Biotech).
Either GST or GST-p14 protein (5 µg) was coupled to 20 µl of
glutathione-Sepharose 4B resin (Amersham Pharmacia Biotech). The resins
were incubated with in vitro translated and
35S-labeled p160 or its derivatives in 100 µl of NETN
buffer (50 mM Tris (pH 7.9), 150 mM NaCl, 0.5%
Nonidet P-40, 1 mM EDTA) for 1 h at 4 °C. After
washing three times with NETN, the bound materials were eluted with 50 µl of SDS sample buffer and analyzed by SDS-PAGE and fluorography.
In Vitro Transcription Assays--
HeLa cell nuclear extract and
the phosphocellulose (P11) column fractions (P.3 and P1.0) were
prepared as described (3). The template plasmid
pTF3-6C2AT, which produces a 380-nucleotide G-free
transcript (3), was used. Transcription reactions were performed as
described (3). In Fig. 3, 20-µl reactions containing P1.0 (2 µl),
P.3 (4 µl), r-p14 (3 ng), r-p160, or a derivative (30 ng) and
pTF3-6C2AT (250 ng) were incubated for 45 min at 30 °C.
Reactions were initiated by adding 2.5 µl of NTP mix (final 60 µM ATP, 600 µM CTP, 5 µM UTP,
80 µM 3'-O-methyl-GTP, and 50 units of RNase
T1) and 2.5 µl of buffer or DRB (final 50 µM). After a
10-min incubation, reactions were terminated and the G-free transcripts
analyzed by 8% urea-PAGE. In Fig. 7, reactions containing HeLa nuclear
extract (4 µl) and p160 Transfection and Luciferase Assays--
HeLa S3 cells were
maintained in minimal essential medium (Nissui) supplemented with 10%
fetal calf serum and L-glutamate. A total of 10 µg of DNA
(5 µg of reporter plasmid, various amounts of pCMV-FLAGp160 Amino Acid Sequence of DSIF p160--
In our previous study, we
reported the isolation of a cDNA encoding the 160-kDa subunit of
DSIF (3). The nucleotide and deduced amino acid sequences of p160 are
shown in Fig. 1A. There was no
ATG sequence upstream of the putative translation initiation site, and
the adjacent sequence fitted the Kozak consensus sequence (25) although
there was no upstream stop codon in frame. To confirm its identity, the
entire cDNA was transcribed and translated in vitro, and
the size of the 35S-labeled product was compared with that
of p160 purified from HeLa nuclear extract and 32P-labeled
by phosphorylation with casein kinase II (Fig. 1B). The
phosphorylation did not change the migration of p160 (data not shown).
From the indistinguishable migration of the two bands, we concluded
that the cDNA encodes full-length p160.
Two groups have reported nucleotide sequences of SUPT5H, a
human homolog of S. cerevisiae SPT5 (26, 27). The p160
cDNA that we have cloned is almost identical to these sequences.
However, the deduced amino acid sequences which we and Stachora
et al. (27) report are different from that reported by
Chiang et al. (26) over two large portions, probably because
of sequencing errors.
p160 Is a Ubiquitously Expressed, Nuclear Protein--
Northern
blot analysis of p160 using mRNA from several different human
tissues detected a ubiquitously expressed band of ~3.6 kilobases, a
length consistent with the isolated cDNA (Fig.
2A). Because p160 possesses
putative nuclear localization signals (Fig. 1A), we analyzed
the subcellular localization of p160. Mammalian vectors expressing
Flag-tagged p160 (Flag-p160) and HA-tagged p14 (HA-p14) were
transfected into HeLa cells, and their expression was examined by
immunofluorescence microscopy. p14/Supt4h is localized in the nucleus
(13).2 As shown in Fig.
2B, Flag-p160 was also exclusively localized in the nucleus,
regardless of co-expression of HA-p14. These results agree with the
postulated role of p160 as a general transcription elongation
factor.
p160 Forms a Stable Complex with p14--
Spt5 genetically and
physically interacts with Spt4 in yeast (16, 28). In addition, p160 was
co-fractionated with p14 through several different columns during
purification of DSIF (3). It is therefore likely that p160 associates
with p14. To test this, we expressed Flag-p160 and HA-p14 in HeLa cells either individually or in combination, and immunoprecipitated them with
anti-Flag antibody. As shown in Fig.
3A, HA-p14 was co-precipitated
with Flag-p160 (lane 8), demonstrating their interaction in vivo.
The interaction was verified in vitro. GST-p14 affinity
column or a control GST column was incubated with in vitro
translated and 35S-labeled p160 under various conditions.
After extensive washing with the same buffer, bound p160 was eluted and
analyzed. About 50% of p160 bound to the GST-p14 column, whereas no
binding to the control column was detected (Fig. 3B,
lanes 2 and 3). The interaction was unaffected by
the presence of 0.5% Nonidet P-40 and 1 M KCl (lane
6). Because p14 contains a putative zinc finger motif conserved
among different species (13), we examined the effect of divalent
cations (lane 4) and a high concentration of a chelating
agent (lane 5). Even under these conditions, p160 efficiently bound to p14, suggesting that the zinc finger is not required for the interaction.
We next mapped the region of p160 involved in p14-binding. Various
forms of 35S-labeled p160 were produced in
vitro, and their interactions with GST-p14 were analyzed (Fig.
3C). The C-terminal half of p160 was dispensable for the
interaction (lanes 8 and 9), and fine mapping of
the N terminus identified a minimal region of aa 176-313 sufficient
for p14-binding (lane 30). Because aa 1-270 of p160 also
bound to p14 equally well (lane 28), the minimal domain is aa 176-270.
Mapping the Region of p160 Important for DSIF Activity in
Vitro--
We next sought to identify regions important for DSIF
activity. We constructed a series of p160 mutants lacking various
structural motifs (see "Materials and Methods"). These mutants were
expressed in E. coli and purified, and the integrity of the
recombinant proteins was verified by either silver staining (Fig.
4A) or immunoblotting with an
antibody raised against the extreme C terminus of p160 (data not
shown).
Equal amounts (30 ng) of these mutants were assayed for DRB
sensitivity-inducing activity (Fig. 4B). This protein amount
corresponds to the "low dose" of our previous report (3). Wild type
r-p160, in conjunction with r-p14, converted DRB-insensitive
transcription to a process sensitive to DRB (lanes 1-4), as
reported previously (3). Analysis of the Interaction between p160 and pol II--
p160/Spt5
physically interacts with pol II (3, 16). In addition, Spt5 genetically
interacts with the two largest subunits of pol II in yeast (16). We
therefore wished to determine which subunit of pol II is involved in
interaction with p160. Pol II purified from HeLa cell nuclear pellet
was separated by SDS-PAGE, transferred to polyvinylidene difluoride
membrane, renatured, and probed with 35S-labeled p160. As
shown in Fig. 5A, a clear band
of ~200 kDa, which co-migrates with the largest subunit of pol II,
was detected. p160 also reacted with GST-p14 protein which was
similarly blotted onto the membrane. From these results, we conclude
that p160 interacts with pol II through its largest subunit pol
IIa.
We next mapped the region of p160 involved in pol II binding. Various
forms of p160 were synthesized in vitro, and incubated with
purified pol II and anti-CTD antibody. The immunocomplexes were
precipitated, and the presence of p160 was analyzed. As shown in Fig.
5B, the C-terminal deletion of p160 did not affect the interaction (lanes 1-8). One predicted role for the KOW
motif is interaction with RNA polymerases (20). Fine mapping, however, revealed that aa 313-420 of p160, an N-terminal region in close proximity to the KOW motifs, is sufficient for pol II binding (lane 23). aa 420-757 of p160, which contains all four of
the KOW motifs, also bound to pol II weakly (lane 24).
Results of deletion analyses described above are summarized in Fig.
6
Identification of a p160 Mutant That Represses DSIF Activity in a
Dominant-Negative Manner in Vitro--
Because DRB is a
nonphysiological compound, it is important to address the role of DSIF
in the absence of DRB. Functional analyses of DSIF to date have been
limited to in vitro experiments, and there is no evidence
regarding its role in vivo. As a first step, we sought to
isolate a dominant-negative mutant of p160. We speculated that p160
mutants that complex with p14, but have no DSIF activity, would act in
a dominant-negative manner. We employed one such mutant, DSIF Represses Transcription from Various Promoters in
Vivo--
Next, we used Structure of DSIF p160--
Results of deletion analyses are
summarized in Fig. 6. Functions of p160 to interact with p14 and pol II
were assigned to different domains between the acidic region and the
KOW motifs. p160-p14 interaction was shown to be very stable, remaining
unaffected by 1 M KCl or 0.5% Nonidet P-40 (Fig.
3B). However, we could not find any protein-protein
interaction motifs within the p14-binding domain. Because this region
is relatively hydrophobic (Fig. 6, top), hydrophobic
interactions may contribute to the stable interaction between p160 and
p14. This interaction seems dispensable for p160-pol II interaction
because p160 mutants lacking the p14-binding domain can still bind to
pol II (Fig. 5).
The C-terminal repeat sequences were dispensable for DSIF activity
in vitro (Fig. 4). Analysis in yeast, however, has
demonstrated that deletion of the C-terminal part of SPT5
impairs complementation of Spt Negative Versus Positive Role of DSIF--
In this study, we
demonstrated that DSIF acts as a negative regulator, at least on some
promoters, in vivo.
SPT4 and SPT5, yeast counterparts of the DSIF
subunits, have been identified as extragenic suppressors of
In Fig. 4, p160 wild-type and the Analysis Using a Dominant-Negative Mutant--
In yeast,
overexpression of SPT5 using a 2µ plasmid leads to Spt
It was estimated that the reporter expression was stimulated
several-fold in parallel with the reduction of endogenous DSIF activity. This indicates that the endogenous DSIF generally represses transcription from the reporter gene; if the endogenous DSIF influenced only part, say 10%, of the transcription, the dominant-negative effect
would not be observed. This is consistent with the idea that DSIF
generally affects transcription of the class II genes, as expected from
its involvement in DRB action.
DSIF subunits are also implicated in other cellular processes such as
chromatin structure, cell cycle, and HIV replication (27, 36; reviewed
in Refs. 1 and 34). The dominant-negative mutant of p160 isolated here
would be a useful tool to examine possible DSIF involvement in these events.
-D-ribofuranosylbenzimidazole
(DRB) is a classic inhibitor of transcription elongation by RNA
polymerase II (pol II). We have previously identified and purified a
novel transcription elongation factor, termed DSIF (for DRB
sensitivity-inducing factor), that
makes transcription sensitive to DRB. DSIF is composed of 160- and
14-kDa subunits, which are homologs of the Saccharomyces cerevisiae transcription factors Spt5 and Spt4. DSIF may either repress or stimulate transcription in vitro, depending on
conditions, but its physiological function remains elusive. Here we
characterize the structure and function of DSIF p160. p160 is shown to
be a ubiquitous nuclear protein that forms a stable complex with p14 and interacts directly with the pol II largest subunit. Mutation analysis of p160 is used to identify structural features essential for
its in vitro activity and to map the domains required for its interaction with p14 and pol II. Finally, a p160 mutant that represses DSIF activity in a dominant-negative manner is identified and
used to demonstrate that DSIF represses transcription from various
promoters in vivo.
INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
REFERENCES
MATERIALS AND METHODS
acidic, which lacks amino acids (aa) 1-175 of p160;
pET-p160
p14BD, which lacks aa 176-314 of p160; pET-p160
NusG,
which lacks aa 314-516 of p160; and pET-p160
repeat, which lacks aa
758-936 of p160.
KOW1 and
KOW2, which lack aa 421-447 and
aa 473-499 of p160, respectively, a polymerase chain reaction-based overlap extension technique was employed (21). Forward and reverse mutagenic primers encompassing the deletion points were used and the
amplified fragments cloned into pET-14b, generating pET-p160
KOW1 and
pET-p160
KOW2. For expression of GST-p14, a fragment of pBS-DSIFp14 was subcloned into pGEX-5X-3 (Amersham Pharmacia Biotech) to generate pGEX-p14. To construct pCMV-FLAGp160 and pCMV-FLAGp160
NusG,
fragments of pBS-FLAGp160 and pBS-FLAGp160
NusG were inserted into
pCAGGS (22). To construct pCMV-HAp14, p14 full-length cDNA was
inserted into pCHA, a pCAGGS derivative that contains a sequence
encoding HA epitope YPYDVPDYA.
NusG mutant (0-200 ng) were incubated for
the indicated times, and processed as above.
NusG,
and the empty vector pCAGGS) were transfected by a standard calcium
phosphate method (24) into 3 × 105 cells plated on
6-cm dishes 20 h before transfection. DNA-CaPO4 co-precipitates were removed 6 h later. The cells were harvested 48 h post-transfection, and their luciferase activities were
measured. The values were normalized by protein amount and expressed as -fold activation.
RESULTS
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Fig. 1.
A, nucleotide and amino acid sequences
of DSIF p160. The nucleotide sequence was deposited in the
GenBankTM/EBI Data Bank under accession number AB000516.
Residues identified by sequencing purified p160 (3) are
underlined. The putative nuclear localization signals are
double underlined. The acidic region is indicated by an
arrow, and acidic residues within this region are
circled. Four stretches with similarity to the KOW motif of
E. coli NusG are named KOW1 to 4 and indicated by
arrows. The hexapeptide repeat sequences are indicated by
open boxes. B, comparison of in vitro
translation (IVT) product of the cDNA and purified p160.
The entire cDNA was transcribed and translated in vitro,
and the size of the 35S-labeled product was compared with
that of p160 purified from HeLa nuclear extract and
32P-labeled by phosphorylation with casein kinase II.
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Fig. 2.
Analysis of the expression pattern of
p160. A, Northern blot analysis. A multiple tissue
Northern blot (CLONTECH) containing 2 µg of
poly(A)+ mRNA per lane was probed for p160 expression.
Northern blotting was performed using a standard technique (24) with a
random-labeled 417-base pair fragment (from 1528 to 1944 nucleotides)
of the p160 cDNA. A human -actin probe
(CLONTECH) was also used to confirm equivalent
loading of intact RNA. The positions of size markers (in kilobases) are
indicated at left. B, subcellular localization of
p160. pCMV-FLAGp160 and pCMV-HAp14 were, either individually or in
combination, transfected into HeLa cells plated onto coverslips. The
cells were fixed with methanol-acetic acid (3:1) solution and
sequentially incubated with 5% milk-phosphate-buffered saline,
anti-Flag antibody, rhodamine-conjugated secondary antibody (Chemicon),
and 0.01 mg/ml 4',6'-diamidino-2-phenylindole (DAPI).
Fluorescence microscopy was carried out using an Axiovert (Carl
Zeiss).
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Fig. 3.
p160 forms a stable complex with p14.
A, in vivo interaction. pCMV-FLAGp160 and
pCMV-HAp14 were transfected into HeLa cells either individually or in
combination. The cell lysates were immunoprecipitated with anti-Flag
antibody. The lysates (10% of the input, lanes 1-4), and
the immunoprecipitated materials (lanes 5-8) were analyzed
by immunoblotting with anti-Flag and anti-HA antibody. B,
in vitro interaction. 35S-labeled p160 was
incubated with either GST-p14 affinity column (lanes 3-6)
or control GST column (lane 2) under various conditions.
After extensive washing with the same buffer, bound p160 was eluted and
analyzed. Lane 1 represents 20% of the input. The binding
and washing conditions were as follows: NETN buffer (lanes 2 and 3); NETN + 10 mM ZnCl2, 10 mM MgCl2 (lane 4); NETN + 10 mM EDTA (lane 5); NETN + 1 M KCl
(lane 6). C, mapping the region of p160 involved
in p14-binding. Various forms of 35S-labeled p160 were
produced in vitro, and their interactions with GST-p14 were
analyzed as in panel B. Results are summarized in
panel D.
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Fig. 4.
Mapping the region of p160 important for DSIF
activity in vitro. A, SDS-PAGE
analysis of a series of p160 deletion mutants. r-p160 wild type and
various mutants were expressed and purified from E. coli.
Aliquots of these proteins (20 ng) were subjected to 7.5% SDS-PAGE and
stained with silver. The positions of size markers are indicated at
left. B, DRB sensitivity-inducing activity of the
p160 mutants. r-p160 wild type and mutants were assayed for their DSIF
activity, as shown in panel C. Three different preparations
of the recombinant proteins were tested except for p14BD, and the
representative experiment is shown. Results are summarized in
panel D.
repeat similarly induced DRB-sensitivity
(lanes 11 and 12). These proteins also moderately
reduced the basal level of transcription, i.e. in the
absence of DRB. In contrast, all other mutants had no effect
(lanes 5-10 and 13-16). The slight variations seen in
p14BD and
NusG were not reproducible. The acidic region, the p14-binding region, and the NusG-homology regions of p160, therefore, appear to be important for DSIF activity.
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Fig. 5.
Analysis of the interaction between p160 and
pol II. A, p160 interacts with the largest subunit of
pol II. pol II was purified from HeLa cell nuclear pellet as described
(37). pol II, GST-p14, and GST (200 ng each) were subjected to
SDS-PAGE, transferred to polyvinylidene difluoride membrane, and
renatured as described (38). The membranes were incubated with in
vitro translated and 35S-labeled p160, and
p160-interacting proteins were visualized by fluorography (Far
Western). The same gel was stained with Coomassie Brilliant Blue
(CBB). B, mapping the region of p160 involved in
pol II-binding. In vitro translated p160 wild type or its
derivatives were incubated with purified pol II (250 ng) and anti-CTD
(8WG16) monoclonal antibody (BabCo) (2 µg) for 2 h at 4 °C in
a buffer containing 20 mM HEPES (pH 8.0), 20% glycerol,
100 mM KCl, 0.5 mM EDTA, and 1 mM
dithiothreitol. The immunocomplexes were precipitated with protein
G-Sepharose, washed three times with the same buffer, eluted with SDS
sample buffer, and analyzed by SDS-PAGE and fluorography. Input
(In) lanes represent 10% of the inputs. C,
summary of the results. Relative binding to pol II is calculated using
NIH Image software and is indicated at right. Wild type
binding is expressed as 100. The positions of the KOW motifs are
indicated by black boxes.
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Fig. 6.
A modular structure of p160. Results of
deletion analyses are schematically summarized. Regions required for
p14-binding, pol II-binding, and DSIF activity are indicated with
thick bars. Also shown at the top is a Kyte-Doolittle
hydropathy plot (39).
NusG, and
tested its dominant-negative effect in vitro. Increasing
amounts of the mutant protein were incubated with HeLa nuclear extract
for 45 min, and then transcription reactions were carried out in the
presence or absence of DRB (Fig. 7A). Addition of 200 ng of the
mutant slightly reduced DRB-sensitivity of the nuclear extract
(lanes 7 and 8). Furthermore, when the preincubation time was prolonged to 180 min, DRB sensitivity was markedly reduced (Fig. 7B, lanes 11 and
12). The nuclear extract used contains ~30 ng of
endogenous p160 protein (3). Thus, 6- to 7-fold excess of the p160
mutant protein, and a 3-h incubation period, were sufficient to
inactivate most of the endogenous DSIF. It is likely that during this
period, endogenous wild type p160 is replaced by the mutant to form an
inactive DSIF complex with p14. These results also suggest that the
p160 mutant is inactive, not simply because of incorrect folding, but
because a specific function of p160 encoded within the deleted region
has been lost.
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Fig. 7.
Identification of a p160 mutant that
represses DSIF activity in a dominant-negative manner in
vitro. A and B, indicated amounts
of p160 NusG protein were incubated with HeLa nuclear extract
(NE) and template for the indicated times, and transcription
reactions were carried out as shown at the top. The
transcripts were quantified using a BAS image analyzer (Fuji). The
relative inhibition by DRB was calculated and shown below.
NusG to analyze the effect of DSIF on
transcription in vivo. Four different reporter plasmids were
used in which expression of the luciferase gene is controlled by the
adenovirus E4 promoter, the HIV-1 long terminal repeat (Fig.
8A, LTR), the rat somatostatin
(som.) promoter, and the mouse metallothionein (metal.) promoter. These promoters possess a typical TATA
box but no "initiator" consensus sequence and are subject to
regulation by transactivators of different classes. The E4 promoter is
activated by ATF and GABP (23, 29); the HIV-1 long terminal repeat is activated by NF-
B, Sp1, and Tat (30); the somatostatin promoter is
stimulated by the c-AMP signaling pathway and CREB (31); and the
metallothionein promoter is induced by heavy metal and MTF-1 (32). One
of these reporter plasmids (5 µg) was co-transfected with increasing
amounts of a plasmid expressing
NusG into HeLa cells. The total
amount of DNA added per transfection was adjusted to 10 µg with the
empty vector pCAGGS.
NusG stimulated luciferase expression 5- to
10-fold in a dose-dependent manner, irrespective of the
promoter used (Fig. 8A). The same transfectants were
examined for expression of the
NusG protein. Immunoblotting with
anti-p160 antibody detected the band of
NusG just below the
endogenous p160 (Fig. 8B). Provided that the reporter and
the effector plasmids were introduced and expressed in 30% of the
total cells, as estimated by immunostaining (not shown), approximately
1-, 3-, and 10-fold excesses of the mutant protein were obtained
(lanes 2-4). These levels are consistent with that at which
the dominant-negative effect is observed in vitro (Fig. 7).
Taken together, these results indicate that DSIF represses
transcription from these promoters in vivo, and reduction of
the endogenous DSIF activity leads to derepressed transcription.
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Fig. 8.
DSIF represses transcription from various
promoters in vivo. A, reporter plasmid
(5 µg) containing either adenovirus E4 promoter (E4), HIV
long terminal repeat (LTR), mouse somatostatin promoter
(som.), or mouse metallothionein promoter
(metal.) was co-transfected with pCMV-FLAGp160 NusG (0.2, 1, 5 µg) into HeLa cells. The total amount of DNA per transfection
was adjusted to 10 µg with pCAGGS. Luciferase activities were
normalized by protein amount and expressed as -fold activation.
B, the same representative samples were also examined for
expression of p160. The cell lysates (10 µg) were analyzed by
immunoblotting with anti-p160 monoclonal antibody (3). The positions of
the expressed mutant and the endogenous (endo.) p160 are
indicated by arrows.
DISCUSSION
phenotype (15). It is
therefore possible that the C-terminal repeats play some regulatory
roles in vivo. Interestingly, this portion is very rich in
Ser, Thr, and Tyr and has many phosphorylation sites for several
protein kinases. Thus, DSIF activity may be regulated by
phosphorylation in vivo (27). On the other hand, most of the
N terminus was required for DSIF activity. Specifically, deletion of
either the acidic region, the p14-binding domain, or one of the two
N-terminal KOW motifs abolished p160 function. At present, however, we
could not assign functions to the acidic region or the KOW motifs.
These elements may be involved in interactions with unidentified partners.
insertion mutations of HIS4 or LYS2 genes (14, 15, 33; reviewed in Ref. 34). When the
sequence is inserted in the
upstream region of a gene, the transcription signal directs
transcription from the
promoter and interferes with normal
transcription of the adjacent gene. Another mutation in SPT4
or SPT5 gene suppresses the aberrant transcription,
restoring transcription from the normal site. A possible interpretation
of this result is that Spt4-Spt5 up-regulates transcription from the
, and down-regulates transcription of the adjacent gene. We
therefore do not discount the possibility that DSIF also functions as a
positive regulator on some promoters that we have not tested.
repeat mutant not only induced
DRB sensitivity but moderately repressed transcription in the absence
of DRB. In fact, both phenomena are often observed at the same time. We
postulate that the DRB sensitivity-inducing activity (low doses) and
the repression activity (high doses) described previously (3) are
inseparable functions of DSIF, and both are related to P-TEFb.
Recently, we found that DSIF and P-TEFb possess opposite functions and
act antagonistically on pol II elongation. In the absence of P-TEFb
activity, DSIF strongly represses transcription regardless of the
presence or absence of DRB (35). Thus, in our current model, DRB
inhibits P-TEFb from alleviating the negative effect of DSIF (1, 35).
The requirement for DRB in the repression by DSIF may vary with the ratio of DSIF to P-TEFb.
phenotype (15). The expression level of Spt5 is presumably important
for its function. Thus, data obtained from overexpression experiments
might prove difficult to interpret. We did in fact perform experiments
of that kind and could not observe any clear-cut effect of wild type
p160.2 We therefore used a dominant-negative mutant of p160
to probe the in vivo function of DSIF in this study.
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ACKNOWLEDGEMENTS |
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We thank Drs. M. Hagiwara, A. Shimomura, T. Nishigaki, J. Sawada, A. Berk, and K. Hisatake for providing plasmids; Dr. D. Price for helpful discussions; Dr. T. Kishimoto's lab for help with the fluorescence microscopy; and Dr. C. Sawa for assistance with Northern blotting.
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FOOTNOTES |
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* This work was supported by a Grant-in Aid for Scientific Research on Priority Areas from the Ministry of Education, Science, Sports and Culture of Japan (to H. H and T. W.), a grant from Yazaki Memorial Foundation for Science and Technology, a Research Grant from CREST of Japan Science and Technology Corporation, and a grant from New Energy and Industrial Technology Development Organization (to H. H.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AB000516.
Research Fellow of the Japan Society for the Promotion of Science.
§ To whom correspondence should be addressed. Tel.: 81-45-924-5797; Fax: 81-45-924-5834; E-mail: hhanda{at}bio.titech.ac.jp.
2 Y. Yamaguchi and H. Handa, unpublished data.
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ABBREVIATIONS |
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The abbreviations used are:
DRB, 5,6-dichloro-1--D-ribofuranosylbenzimidazole;
pol II, polymerase II;
DSIF, DRB sensitivity-inducing factor;
GTF, general
transcription factor;
P-TEFb, positive transcription elongation factor
b;
CTD, C-terminal domain;
aa, amino acids;
HA, hemagglutin;
PAGE, polyacrylamide gel electrophoresis;
GST, glutathione
S-transferase.
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REFERENCES |
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