The Proteoglycan Lectin Domain Binds Sulfated Cell Surface Glycolipids and Promotes Cell Adhesion*

Ryu MiuraDagger , Anders AspbergDagger §, Iryna M. EthellDagger , Kazuki HagiharaDagger , Ronald L. Schnaar, Erkki RuoslahtiDagger , and Yu YamaguchiDagger parallel

From the Dagger  Burnham Institute, La Jolla, California 92037 and the  Department of Pharmacology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

    ABSTRACT
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The lecticans are a group of chondroitin sulfate proteoglycans characterized by the presence of C-type lectin domains. Despite the suggestion that their lectin domains interact with carbohydrate ligands, the identity of such ligands has not been elucidated. We previously showed that brevican, a nervous system-specific lectican, binds the surface of B28 glial cells (Yamada, H., Fredette, B., Shitara, K., Hagihara, K., Miura, R., Ranscht, B., Stallcup, W. B., and Yamaguchi, Y. (1997) J. Neurosci. 17, 7784-7795). In this paper, we demonstrate that two classes of sulfated glycolipids, sulfatides and HNK-1-reactive sulfoglucuronylglycolipids (SGGLs), act as cell surface receptors for brevican. The lectin domain of brevican binds sulfatides and SGGLs in a calcium-dependent manner as expected of a C-type lectin domain. Intact, full-length brevican also binds both sulfatides and SGGLs. The lectin domain immobilized as a substrate supports adhesion of cells expressing SGGLs or sulfatides, which was inhibited by monoclonal antibodies against these glycolipids or by treatment of the substrate with SGGLs or sulfatides. Our findings demonstrate that the interaction between the lectin domains of lecticans and sulfated glycolipids comprises a novel cell substrate recognition system, and suggest that lecticans in extracellular matrices serve as substrate for adhesion and migration of cells expressing these glycolipids in vivo.

    INTRODUCTION
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The lecticans are a family of chondroitin sulfate proteoglycans (CSPGs)1 characterized by the presence of a C-type lectin domain in their core proteins (1, 2). The C-terminal globular domains of lecticans, or "proteoglycan lectin domain" (PLD), consist of one or two epidermal growth factor (EGF)-like domains, a C-type lectin domain, and a complement regulatory protein (CRP) domain. This arrangement of domains is similar to that of selectins, suggesting that lecticans are also involved in the recognition of carbohydrate ligands.

Lecticans are the most abundantly expressed family of proteoglycans in the nervous system. The lectican family includes aggrecan (3), versican (4), neurocan (5), and brevican (6), all of which are expressed in the nervous system at certain stages of development (1, 7). Although aggrecan and versican were initially characterized as connective tissue proteoglycans, their expression in the nervous system has been demonstrated in a number of reports (8-12). Brevican and neurocan are specifically expressed in the nervous system (6, 13-15). Structural similarities with selectins and the abundant expression in the nervous system suggest that lecticans play major roles in carbohydrate recognition in the nervous system.

The identity of the ligand to PLDs has been a focus of our interest. We previously showed that the PLD of versican binds tenascin-R, an extracellular matrix (ECM) protein predominantly expressed in the nervous system (16). More recently, we demonstrated that the PLDs of all lecticans bind tenascin-R, and that brevican and tenascin-R indeed form a complex in adult rat brain extracts (17). These results suggest that the lectican-tenascin-R interactions, especially the brevican-tenascin-R interaction, are relevant to the assembly of the adult brain ECM. However, these interactions are not carbohydrate-protein interactions expected of C-type lectin domains; they are protein-protein interactions between the PLDs and fibronectin type III domains (FNIII) 3-5 of tenascin-R (17). Regarding carbohydrate interactions by PLDs, several studies have demonstrated that PLDs can bind simple sugars and heparin/heparan sulfate in vitro (18-20). However, these studies failed to identify the nature of any physiological carbohydrate ligands for PLDs. Thus the ability of PLDs to behave as C-type lectins in vivo and the identity of physiological carbohydrate ligands for PLDs are issues that have not been addressed.

We have previously shown that purified brevican binds to the surface of primary astrocytes as well as of several immortalized rat neural cell lines (21). Binding studies with B28 glial cells demonstrated that the binding is mediated by the C-terminal 80-kDa fragment of the brevican core protein which includes the PLD (21). It was initially suspected that tenascin-R deposited to the surface of these cells may act as the "receptor" for brevican. However, we have found that these cells do not have any tenascin-R on their surface nor did they secrete any tenascin-R into culture supernatants. Furthermore, a number of assays failed to identify any cell surface protein that specifically binds brevican PLD. Since it has been reported that the lectin domains of P- and L-selectins bind sulfated glycolipids, such as sulfatides (22) and HNK-1-reactive sulfoglucuronylglycolipids (SGGLs) (23), we examined the possibility that the cell surface brevican receptor is a glycolipid rather than a glycoprotein. In this paper, we report that the PLDs of lecticans bind these sulfated glycolipids. We also show that the interaction between brevican and sulfated glycolipids supports adhesion of cells expressing these glycolipids on their surfaces. These observations suggest that the PLD-sulfated glycolipid interactions are a novel cell substrate recognition system.

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Materials-- Mixed bovine brain gangliosides, purified bovine brain sulfatides, and galactosylceramides were purchased from Sigma. Mixed neutral glycosphingolipids were obtained from Matreya (Pleasant Gap, PA). The HNK-1 monoclonal antibody was purchased from Becton Dickinson (Bedford, MA). Mouse monoclonal anti-tenascin-R antibody (clone 596) (24) and monoclonal anti-sulfatide antibody were gifts from Drs. Melitta Schachner (University of Hamburg, Hamburg, Germany) and Yoshio Hirabayashi (RIKEN, Wako, Japan), respectively. The Fc fusion protein of L-selectin (L-selectin Ig chimera) and a HeLa cell line transfected with cDNAs for HNK-1 sulfotransferase, glucuronyltransferase, and N-CAM were obtained from Dr. Minoru Fukuda (Burnham Institute, La Jolla, CA).

Recombinant Proteins-- A chimeric protein of brevican PLD fused with Fc region of human IgG (brevican PLD chimera) and biotinylated recombinant lectin domains (rCLDs) of all four lecticans were prepared as described previously (17). Brevican PLD chimera consists of a short segment of the central domain, a EGF domain, a lectin domain, and a CRP domain of rat brevican. rCLDs consist only of the lectin domains.

Preparation of Authentic, Full-length Brevican-- For preparation of recombinant brevican with no fusion partner, CHO cell were stably transfected with an expression vector pcDNA-rBV, which has a full-length rat brevican cDNA (3.0-kilobase pair EcoRI-EcoRI fragment) (25) inserted into a unique EcoRI site of pcDNAIAmp (Invitrogen, San Diego, CA). For the preparation of radiolabeled probe for TLC-ligand binding assay, cultures of a cloned CHO transfectant were metabolically labeled with 100 µCi/ml Tran35S-Label (ICN, Costa Mesa, CA). 35S-Labeled or unlabeled brevican was purified from culture supernatants by affinity chromatography on the FNIII3-5 fragment of tenascin-R as follows. The glutathione S-transferase fusion protein of the FNIII3-5 fragment (17) was bound to glutathione-agarose and covalently coupled with dimethylpimelimidate according to Gersten and Marchalonis (26). Culture supernatants of CHO transfectants were incubated overnight at 4 °C with the FNIII3-5 affinity resin preequilibrated with TBS containing 5 mM CaCl2. After extensive washing, the labeled brevican was eluted from the affinity resin with 20 mM EDTA.

Flow Cytometry, Immunoblotting, and Ligand Overlay Assays-- For flow cytometry, cells were dissociated with trypsin-EDTA (Irvine Scientific, Irvine, CA), suspended in 10% fetal calf serum in Dulbecco's modified Eagle's medium, and incubated in the medium for 2 h at 37 °C. Cell were then washed three times with PBS containing 0.1% sodium azide, and incubated with PBS containing 1% BSA and 0.1% sodium azide for 20 min on ice. Cells were then incubated with brevican PLD chimera or primary antibodies for 30 min on ice. After washing three times with PBS containing 0.1% sodium azide, the cells were incubated with fluorescein-conjugated goat antibodies to human IgG (Sigma) or to mouse IgG+IgM (Biosource) for 20 min on ice, washed again, and resuspended in 0.5-1.0 ml of PBS containing 0.1% sodium azide. The cells were examined on a FACSort (Becton Dickinson, Oxford, CA). Cell surface biotinylation was performed with sulfo-NHS-biotin (Pierce) according to the manufacture's instruction. Biotinylated molecules were collected by streptavidin-agarose. Ligand overlay and immunoblotting assays were performed as described previously (17).

Preparation of Glycolipids-- For preparation of total glycolipid fraction, tissues or cells were extracted by sonication with chloroform/methanol (2:1) and then with a chloroform/methanol/water (1:2:0.8). After removal of the remaining precipitates by centrifugation, the supernatant was dried under N2 stream. The resulting residue was dissolved in chloroform/methanol (2:1), and then 1/10 volume of 2 M KOH in methanol was added. The solution was incubated at 37 °C for 2 h to degrade phospholipids. The supernatant was collected by centrifugation, neutralized with 1/20 volume of acetic acid, and dried under N2 stream. The residue was suspended in water, dialyzed against water, evaporated to dryness, and then dissolved in chloroform/methanol/water (60:35:8). Acidic glycolipids were purified as described previously (27). HNK-1-reactive SGGLs were purified from dog sciatic nerve endoneurium as described previously (28). This sample contains 41% SO3-GlcU-nLc-4-Cer and 59% SO3-GlcU-nLc-6-Cer, both of which are recognized by anti-HNK-1 antibody.

Thin-layer Chromatography (TLC) Ligand Binding Assays-- These assays were performed according to Taki et al. (29). Briefly, glycolipid samples were developed twice on high performance thin layer chromatography plates (Baker Inc., Phillipsburg, NJ) in chloroform/methanol/0.2% aqueous CaCl2 (60:35:8). After drying, the plates were dipped for 30 s in 0.4% polyisobutylmethacrylate (2.5% polyisobutylmethacrylate in chloroform was diluted with hexane to the final concentration of 0.4%), and air-dried. The dried plates were then incubated with various probes (antibodies, brevican PLD chimera, rCLDs, or 35S-labeled native brevican) in TBS containing 1-3% BSA at 4 °C overnight. After washing with TBS, the plates were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies or HRP-conjugated avidin for 1.5 h, followed by the visualization of reactive bands with chemiluminescence. In the case of 35S-labeled brevican, the dried plates were directly exposed to Kodak BioMax film for 3 days.

Cell Adhesion Assay-- For preparation of substrates, solutions of brevican PLD chimera or human IgG (100 µg/ml) in calcium- and magnesium-free Hank's balanced salt solution (CMF/HBSS) were applied on nitrocellulose-coated plastic (21), and incubated at 37 °C for 2 h. After washing three times with CMF/HBSS, uncoated surfaces were blocked by incubating with CMF/HBSS containing 2% heat-inactivated BSA (HBSS/BSA) for 2 h at 37 °C. After washing three times with CMF/HBSS, cells suspended in Opti-MEM (Life Technologies, Inc.) containing 0.1% heat-inactivated BSA were plated at a density of 5 × 105 (B28 cells), or 1 × 106 (MDCK cells) per ml, and incubated for 1 h at 37 °C. After gentle washing, attached cells were fixed with 4% paraformaldehyde in PBS and counted under microscope at 200× magnification. For perturbation with antibodies, cells were preincubated with 100 µg/ml HNK-1 or anti-sulfatide monoclonal antibodies diluted in CMF/HBSS/BSA for 30 min on ice prior to the plating of the cells. For perturbation by glycolipids, the substrate of brevican PLD chimera was preincubated with SGGLs, sulfatide, or galactosylceramide at concentration of 5 µg/ml in CMF/HBSS for 1 h at 37 °C before plating of the cells.

    RESULTS
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Brevican Binds to Cell Surfaces through Non-tenascin-R Binding Sites-- We have shown that the PLD-containing C-terminal 80-kDa fragment of brevican core protein binds to primary astrocytes and B28 cells, which is an immortalized glial cell line. Binding studies with cell monolayers ruled out hyaluronan, heparan sulfate, and chondroitin sulfate as cell surface "receptors" for the brevican C-terminal fragment (21).

To facilitate the identification of the putative brevican receptor, a fusion protein of brevican PLD and Fc region of human IgG (brevican PLD chimera) and a biotin-labeled recombinant lectin domain of brevican (brevican rCLD) were produced. We first examined the binding of brevican PLD chimera to a series of immortalized neural cell lines derived from BDIX rats (30) in flow cytometric assay. Among 16 cell lines tested, brevican PLD chimera bound to B28 cells (Fig. 1A) and four other glial cell lines, namely B9, B15, B49, and B92. The chimera showed no binding to other cell lines with fibroblastic or neuronal phenotypes, including B23 (Fig. 1B), B19, B27, B35, B50, B65, B82, B103, B104, B108, or B111 cells (data not shown). These results suggest that the cell surface binding of the 80-kDa fragment is mediated by the PLD of the brevican core protein, and that the brevican receptor is expressed in various neural cell lines.


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Fig. 1.   Brevican PLD chimera binds B28 cells. A and B, cell surface binding of brevican PLD chimera to B28 (A) and B23 (B) cells analyzed by flow cytometry. Cells were stained with brevican PLD chimera followed by fluorescence-conjugated anti-human IgG (solid line), or with fluorescence-conjugated anti-human IgG only (dotted line). Note that B28 cells bind brevican PLD chimera to their surface but B23 cells do not. C, ligand overlay assay of biotinylated cell surface proteins probed with brevican PLD chimera. Lanes 1-3, B28 cells were surface-biotinylated and biotinylated proteins were resolved in an 8-16% gradient gel under a nonreducing condition. Nitrocellulose filters blotted with the gel were probed with HRP-conjugated avidin (lane 1), brevican PLD chimera followed by HRP-conjugated anti-human IgG (lane 2), or HRP-conjugated anti-human IgG alone (lane 3). Incubation with brevican PLD or anti-human IgG was performed in the presence of 5 mM CaCl2. Lanes 2 and 3 were exposed for extended periods to show any positive bands. Note that brevican PLD chimera does not bind any specific proteins. This result indicates that B28 cells do not contain tenascin-R nor other proteins that specifically interact with brevican PLD on their surface. The band at ~200 kDa (indicated by asterisk) is due to nonspecific binding of HRP-conjugated anti-human IgG (see lane 3). Lane 4, positive control for the reactivity of the brevican PLD chimera. Adult rat brain extracts were resolved by SDS-polyacrylamide gel electrophoresis as described above, blotted to nitrocellulose, and probed with the brevican PLD in the presence of 5 mM CaCl2. Note that the brevican PLD chimera, although it did not bind to any specific proteins in lanes 1-3, recognized tenascin-R present in adult brain extracts (17).

The lectin domain of brevican binds tenascin-R by a protein-protein interaction (17). Although tenascin-R is a secreted protein, it is possible that tenascin-R is present on the surface of B28 cells through interaction with cell surface tenascin-R binding proteins (e.g. contactin) or by nonspecific aggregation, thereby acting as an apparent brevican receptor. However, as described previously (21), no tenascin-R was detected on the surface of B28 cells either by flow cytometric assay or by immunocytochemistry (data not shown). Furthermore, the ligand overlay assay with brevican PLD chimera, which we used to identify tenascin-R as a protein ligand to lectican PLDs in adult rat brain extracts (17), did not detect tenascin-R in surface-biotinylated proteins from B28 cells (Fig. 1C).

Having ruled out tenascin-R as the putative receptor for brevican PLD, we next examined the possibility that cell surface glycoproteins carrying specific carbohydrates would be the receptor for brevican PLD, as is the case with selectins. However, the ligand overlay experiment demonstrated that B28 cells lacks not only tenascin-R but also any cell surface proteins that specifically interact with brevican PLD chimera (Fig. 1C), suggesting that there are no glycoprotein ligands for the brevican PLD in B28 cells. We further searched for cell surface brevican-binding proteins in B28 cells by immunoprecipitation of surface-labeled materials and affinity chromatography on a column bearing the 80-kDa brevican fragment that includes PLD. None of these experiments could identify glycoproteins that would specifically bind brevican PLD (data not shown).

The Proteoglycan Lectin Domain Binds Sulfatides and HNK-1-reactive SGGLs-- It has been reported that sulfated cell surface glycolipids, sulfatides and HNK-1-reactive SGGLs, bind to the lectin domains of P- and L-selectin (22, 23). Therefore, we investigated the possibility that glycolipids would act as cell surface receptors for brevican PLD. To test this, we prepared glycolipids from the adult rat cerebellum and probed with brevican PLD chimera in the TLC-ligand overlay assay. As shown in Fig. 2A, brevican PLD chimera reacted with a single band (lane 2) among a number of glycolipid species extracted from the cerebellum (lane 1). The reactive band migrated at the same position as purified sulfatides (compare panel A, lane 2 with panel B, lane 1), suggesting that the band represents sulfatides. To further identify this reactive band, standard glycolipids were examined by TLC-ligand overlay assay (Fig. 2, B-F) The brevican PLD chimera bound to purified sulfatides (C, lane 1), but not to any of the neutral glycosphingolipids (lane 2) or gangliosides (lane 3). Binding to sulfatides was abolished in the presence of EDTA (D, lane 1), as expected of a C-type lectin interaction. The brevican PLD chimera did not bind galactosylceramide, an unsulfated precursor of sulfatides (indicated by asterisk in B, lane 2), suggesting that a sulfate group is necessary for binding. L-selectin Ig chimera also bound to sulfatides, but not to other glycolipids (E), consistent with previous reports (23). Human IgG did not bind any glycolipids (F).


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Fig. 2.   Brevican PLD binds sulfatide. A, total glycolipids isolated from adult rat cerebellum were analyzed for binding to brevican PLD chimera in TLC-ligand overlay assay. TLC plates were stained by orcinol to visualize total glycolipids (lane 1) or probed with brevican PLD chimera followed by HRP-conjugated anti-human IgG (lane 2). Note that brevican PLD chimera binds to a single glycolipid species (lane 2). This band comigrates with purified specimen of sulfatides (lane 1 in B). B-F, purified glycolipid specimens were analyzed for binding to brevican PLD chimera in TLC-ligand overlay assay. TLC plates were stained by orcinol (B), or probed with brevican PLD chimera in the presence of 5 mM CaCl2 (C), brevican PLD chimera in the presence of 20 mM EDTA (D), L-selectin Ig chimera in the presence of 5 mM CaCl2 (E), or normal human IgG in the presence of 5 mM CaCl2 (F). Lanes 1, bovine brain sulfatide (2.5 µg); lanes 2, mixed neutral glycosphingolipids (10 µg); lanes 3, mixed gangliosides (10 µg). BrePLD, brevican PLD chimera; LS-Ig, L-selectin Ig chimera; hIgG, human IgG. Asterisk in B indicates galactosylceramides. Note that brevican PLD chimera as well as L-selectin Ig chimera binds sulfatide in the presence of Ca2+.

The brevican PLD chimera contains not only the C-type lectin domain but also EGF and CRP domains flanking the lectin domain (17). To examine the location of the sulfatide binding site, the binding of biotinylated recombinant protein consisting of only the lectin domain (brevican rCLD) was examined. Like the PLD chimera, brevican rCLD specifically bound to sulfatides (Fig. 3E, lane 1), but not to neutral glycolipids, including galactosylceramide, or to gangliosides (Fig. 3E, lane 2). Moreover, rCLDs of aggrecan, neurocan, and versican also showed specific binding to sulfatide (Fig. 3, B-D). All of these interactions were completely suppressed in the presence of EDTA (data not shown). These results show that the C-type lectin domain of all four lecticans bind sulfatides in a divalent cation-dependent manner.


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Fig. 3.   Lectin domains of all four lecticans bind sulfatide. Glycolipid samples were separated in TLC and probed with rCLDs of lecticans. TLC plates were stained by orcinol (A), or probed with aggrecan rCLD (B), neurocan rCLD (C), versican rCLD (D), or brevican rCLD (E) in the presence of 5 mM CaCl2. Lanes 1, mixtures of purified sulfatides (2.5 µg) and galactosylceramides (2.5 µg); lanes 2, mixtures of mixed neutral glycosphingolipids (10 µg) and gangliosides (10 µg). GalC, galactosylceramides. Note that rCLD of all lecticans bind to sulfatide, but not to galactosylceramide.

Considering that L- and P-selectins bind another class of sulfated glycolipid, HNK-1-reactive SGGLs (23), we tested the binding of PLDs to the HNK-1-reactive SGGLs. A sample of dog sciatic nerve-derived SGGLs consisting of roughly equal amounts of SO3-GlcU-nLc-4-Cer and SO3-GlcU-nLc-6-Cer was tested for binding to brevican PLD chimera (Fig. 4). The brevican PLD chimera bound to both SO3-GlcU-nLc-4-Cer and SO3-GlcU-nLc-6-Cer (lane 3). The binding was EDTA-sensitive (lane 4). Consistent with previous reports, L-selectin Ig chimera bound SGGLs (lane 5), but human IgG did not (lane 6). These results demonstrate that PLD binds HNK-1-reactive SGGLs.


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Fig. 4.   Brevican PLD binds HNK-1-reactive SGGLs. Purified SGGLs from dog sciatic nerve containing 41% SO3-GlcU-nLc-4-Cer and 59% SO3-GlcU-nLc-6-Cer (5 µg/lane) were separated in TLC, and stained with orcinol (lane 1), or probed with HNK-1 monoclonal antibody (lane 2), brevican PLD chimera in the presence of 5 mM CaCl2 (lane 3), brevican PLD chimera in the presence of 20 mM EDTA (lane 4), L-selectin Ig chimera in the presence of 5 mM CaCl2 (lane 5), or human IgG in the presence of 5 mM CaCl2 (lane 6). HNK-1, HNK-1 monoclonal antibody; BrePLD, brevican PLD chimera; LS-Ig, L-selectin Ig chimera; hIgG, human IgG. Note that brevican PLD chimera binds both species of HNK-1-reactive SGGLs in the presence of Ca2+.

HNK-1-reactive carbohydrates are present on several glycoproteins as well as on glycolipids (31, 32). Thus, there remained a possibility that glycoproteins carrying HNK-1-reactive glycans could bind PLDs and act as PLD receptors. To address this question, we performed two experiments. First, we prepared extracts containing large amounts of HNK-1-positive glycoproteins from E19 mouse brain. These brain extracts indeed contained several protein bands intensely reactive with the HNK-1 antibody in immunoblotting (Fig. 5A, lane 1). We probed this sample with brevican PLD chimera in ligand overlay assay. Brevican PLD chimera, while it efficiently bound to tenascin-R in adult brain extracts (see Fig. 1C, lane 4), did not bind any of these HNK-1-positive glycoproteins (Fig. 5A, lane 2). Second, we examined by flow cytometry the binding of brevican PLD chimera to a HeLa-derived cell line that expresses HNK-1 carbohydrates only on glycoproteins, not on glycolipids. This cell line has been transfected with cDNAs for N-CAM, a glucuronyltransferase (33), and the HNK-1 sulfotransferase (34). The glucuronyltransferase acts only on glycoprotein glycans and not on glycolipids (33). Because the parental HeLa cells express no HNK-1-reactive SGGLs, all of the HNK-1 carbohydrates expressed by these cells are contained on glycoproteins, mainly on N-CAM.2 Flow cytometric analysis confirmed that these cells express high levels of cell surface HNK-1 carbohydrates (Fig. 5B, a). Despite this, these cells do not bind brevican PLD chimera at all (Fig. 5B, b). In contrast, B28 cells, which express HNK-1-reactive SGGL (see below), show strong binding of brevican PLD chimera (see Fig. 1). Although these results do not entirely rule out the possibility that HNK-1 carbohydrates carried by glycoproteins could be recognized by PLD under some conditions, they demonstrate that HNK-1 carbohydrates attached to glycoproteins are not recognized by PLDs as efficiently as HNK-1-reactive SGGLs.


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Fig. 5.   HNK-1 carbohydrates on glycoproteins are not efficiently recognized by brevican PLD. A, a microsome fraction from E19 mouse brain was resolved by SDS-polyacrylamide gel electrophoresis, transferred to nitrocellulose filters, and probed with the HNK-1 monoclonal antibody (lane 1) or brevican PLD chimera (lane 2). Note that brevican PLD chimera does not bind to any of the HNK-1 carrying glycoproteins present in the fraction. B, flow cytometric analysis of a HeLa cell line expressing high levels of glycoprotein-attached HNK-1 carbohydrates driven by HNK-1 sulfotransferase (34). These HeLa cells were incubated with the HNK-1 antibody (a) or brevican PLD chimera (b). Bound chimera or antibodies were detected with fluorescein-conjugated anti-mouse IgG+IgM (a) or anti-human IgG (b). Dotted lines, cells were stained with secondary antibodies alone. Note that brevican PLD chimera does not bind to these cells, although they express high levels of glycoprotein-attached HNK-1 epitope.

Intact Brevican Also Binds Sulfated Glycolipids-- We next examined whether intact, full-length brevican, not just the recombinant PLD fragment, binds these sulfated glycolipids. To test this, we isolated 35S-labeled brevican from culture supernatants of CHO cells transfected with full-length rat brevican cDNA, and used it as a probe in the TLC-ligand overlay assay. As shown in Fig. 6, native brevican bound to both sulfatides and SGGLs (lane 1). No binding to neutral glycosphingolipids or gangliosides was found (lane 2). The glycolipid binding by brevican was inhibited with EDTA (lane 3), as was the case with the PLD chimera and the rCLDs. These results demonstrate that not only recombinant PLD fragment but also intact brevican binds sulfated glycolipids.


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Fig. 6.   Native brevican binds sulfatides and SGGLs. Glycolipid specimens were separated by TLC, and then probed with 35S-labeled brevican prepared from CHO transfectants. Samples were: mixtures of 2.5 µg of sulfatide and 5 µg of SGGLs (lanes 1 and 3) and mixtures of 10 µg of mixed neutral glycosphingolipids and 10 µg of gangliosides (lanes 2 and 4). Assay was performed in the presence of 5 mM CaCl2 (lanes 1 and 2) or 20 mM EDTA (lanes 3 and 4). Note that native brevican binds both sulfatides and HNK-1-reactive SGGLs. The binding is calcium-dependent.

Binding of Brevican PLD Chimera to B28 Cells Is Mediated by HNK-1-reactive Glycolipids-- To determine if the cell surface binding of brevican observed with the B28 cells (see Fig. 1A) (21) is indeed mediated by sulfated glycolipids, we examined whether these cells contain sulfatides and/or SGGLs. Analysis of an acidic glycolipid fraction from B28 cells demonstrated that B28 cells express HNK-1-reactive SGGLs but not sulfatides (Fig. 7A, lanes 2 and 3). Brevican PLD bound to the B28 cell SGGLs (lane 4).


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Fig. 7.   Binding of brevican PLD chimera to B28 cells is mediated by HNK-1-reactive glycolipids. A, B28 cells have HNK-1-reactive SGGLs. Acidic glycolipids were isolated from B28 cells and analyzed in TLC-ligand overlay assay. TLC plates in which B28 cell-derived acidic glycolipids had been separated were stained with orcinol (lane 1), or probed with the HNK-1 antibody (lane 2), anti-sulfatide monoclonal antibody (lane 3), or brevican PLD chimera (lane 4). Note that B28 cells contain HNK-1-reactive SGGLs but not sulfatides (lanes 2 and 3). Brevican PLD chimera binds B28 cell-derived SGGLs (lane 4). B, binding of brevican PLD chimera to B28 cells is inhibited by HNK-1 antibody. B28 cells that had been preincubated with or without the HNK-1 antibody, were incubated with brevican PLD chimera, followed by staining with fluorescein-conjugated anti-human IgG and analysis by flow cytometry. Solid line, binding of brevican PLD chimera to untreated B28 cells; broken line, binding of brevican PLD chimera to B28 cells pretreated with the HNK-1 antibody; dotted line, staining with fluorescein-conjugated secondary antibody alone. Note that the HNK-1 monoclonal antibody abolished the binding of brevican PLD chimera to B28 cells (broken line).

To confirm the role of HNK-1-reactive SGGLs in the cell surface binding of brevican, we examined whether the HNK-1 antibody would inhibit brevican PLD binding to the B28 cells. As shown in Fig. 7B, binding of the brevican PLD chimera to B28 cells was inhibited by preincubation of the cells with the HNK-1 antibody by flow cytometric assay. Taken together, these results indicate that the binding of brevican to B28 cells is mediated by an interaction between HNK-1-reactive SGGLs and the brevican PLD.

Brevican PLD Supports Cell Adhesion through Sulfated Cell Surface Glycolipids-- Our finding that sulfated cell surface glycolipids bind lecticans, which are ECM components, suggests that these proteoglycans may act as adhesive substrates for cells expressing sulfated glycolipids. To test this possibility, we studied the attachment of B28 cells, which express HNK-1-reactive SGGLs (see above), and MDCK cells, which have been shown to express sulfatides (35), on substrates of brevican PLD. The expression of sulfatides by MDCK cells was confirmed by flow cytometric assay and TLC-immunoblotting with anti-sulfatide monoclonal antibody (data not shown). The brevican PLD chimera was adsorbed to nitrocellulose-coated dishes, and cell adhesion to the substrate was quantitatively analyzed. Both the B28 and MDCK cells adhered to the brevican PLD substrate (Fig. 8, panels A1 and B1) but not to a human IgG substrate (panels A5 and B5). Preincubation of cells with the HNK-1 antibody (panel A2) or anti-sulfatide antibody (panel B2) significantly reduced the number of cells that adhered to the brevican PLD substrate. This adhesion was also inhibited by pretreatment of the brevican PLD substrate with purified SGGLs (panel A3) or sulfatides (panel B3) before plating of cells. Pretreatment of the substrates with galactosylceramides did not have any effect on the adhesion of either cell type (panels A4 and B4). Quantitative analysis of these results is shown in Fig. 8 (C and D). These results demonstrate that the brevican PLD-glycolipid interactions can support cell adhesion, and suggest that brevican present in the brain ECM may serve as an adhesive substrate for neural cells expressing HNK-1-reactive SGGLs or sulfatides.


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Fig. 8.   Brevican PLD-glycolipid interactions support cell adhesion. A, adhesion of B28 cells to the substrate of brevican PLD chimera. Cells were plated on the substrate of brevican PLD chimera (panels 1-4) or human IgG (panel 5). In panel 2, cells were preincubated with 100 µg/ml HNK-1 monoclonal antibody before plating. In panels 3 and 4, substrates were preincubated with 5 µg/ml SGGLs or 5 µg/ml galactosylceramide, respectively, before plating. Photographs were taken 1 h after plating. B, adhesion of MDCK cells to the substrate of brevican PLD chimera. The arrangement of the experiment was the same as in A, except that cells were preincubated with anti-sulfatide monoclonal antibody instead of HNK-1 monoclonal antibody in panel 2, and with sulfatide instead of SGGLs in panel 3. C and D, quantitation of cell adhesion. Cell adhesion assays were performed as described above. Number of adhered cells per field was counted under 200× magnification. Results represent the mean ± 1 S.D. of adhered cells from experiments in quadruplicate. Experimental arrangement in columns 1-5 corresponds to those of panels 1-5 in A and B. Bre, brevican PLD chimera; hIgG, human IgG; HNK-1, HNK-1 monoclonal antibody; alpha Sulf, anti-sulfatide monoclonal antibody; Sulf, sulfatides; GalC, galactosylceramide.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

In this paper, we demonstrate that the PLDs of the lectican family proteoglycans bind two types of sulfated cell surface glycolipids, sulfatides and HNK-1-reactive SGGLs, in a divalent cation-dependent manner. Not only the Ig chimera of the brevican PLD but also the intact brevican core protein possesses the same binding activity. These interactions support the adhesion of sulfatide- or SGGL-expressing cells.

Previous reports on the carbohydrate interaction of PLDs have demonstrated that PLDs are capable of binding simple carbohydrates, including fucose, galactose, and N-acetylglucosamine (16, 18, 19), and heparin/heparan sulfate (20). However, these studies failed to provide data indicating biological significance of the carbohydrate interactions or to identify physiological ligands carrying these carbohydrate determinants. The present study for the first time identifies sulfatides and SGGLs as physiologically relevant carbohydrate ligands for PLDs.

The PLDs of lecticans share several binding properties with selectins. Like PLDs, P- and L-selectins have been shown to bind sulfatides and HNK-1-reactive SGGLs (22, 23, 36). Both the PLD-sulfated glycolipid and the selectin-sulfated glycolipid interactions are divalent cation-dependent. The presence of sulfate residues on terminal sugars is required for binding (22, 23, 36). On the other hand, selectins and PLDs bind differently to gangliosides, another class of acidic glycolipids containing sialic acids. P- and L-selectins have been shown to bind not only sulfated glycolipids but also gangliosides (37, 38), whereas we showed that brevican PLD does not bind gangliosides. This suggests that the mere presence of negative charges is not sufficient for the binding of brevican PLD to sulfated glycolipids.

A noteworthy property of PLDs is that they do not recognize glycoproteins carrying HNK-1 carbohydrates (see Fig. 5), whereas they do bind to HNK-1 carbohydrates carried by a glycolipid. There are two possible explanations for this selective binding. PLDs may recognize not only the terminal sulfoglucuronyllactosamine, the structure that is shared by HNK-1-reactive glycolipids and glycoprotein glycans, but also additional sugar residues of glycolipid chains. In this case, it would be necessary for the PLD to recognize more than four sugar residues to distinguish between HNK-1-reactive glycolipids and glycoproteins. This seems unlikely, because functional groups of carbohydrate determinants interacting with selectins are restricted to the three terminal sugar residues (39, 40). It seems more likely that the low density of HNK-1 carbohydrates on neural glycoproteins limits their ability to bind PLDs. In fact, this situation would be similar to what has been encountered with selectins. Ordinary glycoproteins, even if they carry a selectin-binding carbohydrate determinant such as sialyl Lewisx, are weak selectin binders; the presence of clusters of the carbohydrate determinant on a polypeptide backbone is required for efficient selectin binding (41, 42). As a result, essentially all known glycoprotein ligands for selectins are mucin-type glycoproteins that contain clusters of O-linked glycans (43-46). On the other hand, neural glycoproteins that carry HNK-1 carbohydrates are not mucin-type glycoproteins, and therefore presumably are incapable of sustaining high affinity interactions. Although it is possible for PLDs to bind to HNK-1-reactive oligosaccharides when presented at a high density, it is unlikely that neural glycoproteins carrying HNK-1 carbohydrates act as the receptor for the PLDs in vivo.

Several lines of evidence suggest that the PLD-sulfated glycolipid interactions are physiologically significant. For instance, the tissue distribution of lecticans overlaps with the distribution of sulfatides and SGGLs. Sulfatides and SGGLs are enriched in nervous tissues, as are the lecticans. SGGLs are particularly abundant in embryonic cerebral cortex and adult cerebellum (31). Neurocan and brevican are highly expressed in embryonic brain and adult cerebellum, respectively (21, 47). Sulfatides are a major component of myelin (48) and are expressed in oligodendrocytes and Schwann cells in culture (49, 50). Versican has been shown to be expressed in cultured oligodendrocytes and Schwann cells (51, 52) and brevican in oligodendrocytes (53).

Our demonstration that PLD-sulfated glycolipid interactions support cell adhesion also suggests that these interactions have biological significance. Roles for sulfatides and SGGLs in neural cell interactions have been proposed in earlier studies. For example, it has been suggested that SGGLs and sulfatides are involved in the adhesion of dissociated cerebellar cells and outgrowth of neurites and astrocytic processes (54). SGGLs have also been shown to have effects on Schwann cell adhesion (28, 55). More recently, production of higher levels of SGGLs in cerebellar granule neurons has been shown to correlate with enhanced neurite outgrowth from these cells (56). Despite these observations, the identity of the SGGL/sulfatide-binding molecule(s) involved in these adhesion events has been elusive. Although several heparin-binding proteins have been shown to bind sulfatides and SGGLs, including laminin (57, 58), thrombospondin (59), von Willebrand factor (60), tenascin-C (61), tenascin-R (62), and SBP-1 (63), no lectin-type molecule expressed in the nervous system has been identified as a ligand for these sulfated glycolipids. Lecticans, on the other hand, bind sulfatides and SGGLs in the manner expected of C-type lectin interactions and their distribution overlaps with that of SGGLs and sulfatides. Thus, lecticans are strong candidates as physiological ligands for these sulfated glycolipids in nervous tissues. It is possible that at least some of the cell surface interactions reported by the studies cited above are mediated by lecticans.

The fact that the PLD promotes cell adhesion is, at first glance, somewhat perplexing, since chondroitin sulfate can be inhibitory to cell adhesion and neurite outgrowth. In fact, it has been shown that brevican can inhibit neurite outgrowth from cerebellar granule neurons, and that the chondroitin sulfate moiety is responsible for this activity (6). However, it should be noted that lecticans do not always carry chondroitin sulfates. One of the versican splicing variants expressed mainly in the brain lacks domains that contain glycosaminoglycan attachment sites (64). Brevican is a so-called part-time proteoglycan and roughly 20-50% of brevican molecules isolated from whole brain are devoid of chondroitin sulfates (6, 7). Thus, our present findings suggest the possibility that brevican may act as a bifunctional substrate for cell adhesion, depending on whether it contains chondroitin sulfate chains. Such a putative dual effect on cell adhesion suggests that the regulation of chondroitin sulfate chain synthesis could serve as a biological switch determining whether a CSPG is inhibitory or promoting to cell adhesion.

    ACKNOWLEDGEMENTS

We thank Drs. Melitta Schachner, Yoshio Hirabayashi, and Minoru Fukuda for providing reagents and cell lines. We also thank Drs. Fukuda, William Stallcup, and Douglas Ethell for critical reading of the manuscript.

    FOOTNOTES

* This work was supported by National Institutes of Health Grants NS32717 and HD25938 (to Y. Y.) and CA28896 (to E. R.), and by Cancer Center Support Grant CA30199.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Current address: Dept. of Cell and Molecular Biology, Lund University, P. O. Box 94, S-221 00 Lund, Sweden.

parallel To whom correspondence should be addressed: Burnham Institute, 10901 N. Torrey Pines Rd., La Jolla, CA 92037. Tel.: 619-646-3124; Fax: 619-646-3199; E-mail: yyamaguchi{at}burnham-inst.org.

2 M. Fukuda, personal communication.

    ABBREVIATIONS

The abbreviations used are: CSPG, chondroitin sulfate proteoglycan; PLD, proteoglycan lectin domain; CRP, complement regulatory protein; ECM, extracellular matrix; SGGL, sulfoglucuronylglycolipid; rCLD, recombinant C-type lectin domain; FNIII, fibronectin type III; EGF, epidermal growth factor; CHO, Chinese hamster ovary; TBS, Tris-buffered saline; MDCK, Madin-Darby canine kidney; HRP, horseradish peroxidase; PBS, phosphate-buffered saline; BSA, bovine serum albumin; CMF, calcium- and magnesium-free; HBSS, Hank's balanced salt solution.

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