From the Department of Biochemistry, University of
Ottawa, Ottawa, Ontario K1H 8M5, Canada, the § Institute for
Biological Sciences, National Research Council of Canada, Ottawa,
Ontario K1A 0R6, Canada, and the ¶ Memorial Sloan-Kettering
Cancer Center, New York, New York 10021
![]() |
ABSTRACT |
---|
![]() ![]() ![]() ![]() |
---|
The murine antibody R24 and mouse-human
Fv-IgG1( Immunotherapy using monoclonal antibodies
(mAbs)1 to target neoplastic
cells has been shown to shrink the sizes of some tumors and, in some
cases, to induce regression. Successful therapy depends on many
factors, such as the recognition and binding of the antibody to the
tumor antigen, and the subsequent induction of an immune response
leading to tumor cell death.
Research into cell-surface tumor antigens has concentrated on human
melanoma malignancies, and several have been found that can induce
either a humoral or cellular immune response (1, 2). Unfortunately, few
antigens have been identified that are recognized by both components of
the immune system. At least six humoral-mediated melanoma antigens have
been reported to date, of which four have been found to be gangliosides
(1, 2). Gangliosides are glycolipids consisting of a ceramide tail with an oligosaccharide head group and have long been implicated in cellular
interactions, differentiation and oncogenesis (3). The ceramide moiety
is sequestered within the membrane, leaving the carbohydrate moiety
exposed to immune surveillance. One challenge encountered in exploiting
some gangliosides as tumor antigens can be poor observed
immunogenicity. This may be due, in part, to a limited presence of the
ganglioside on the surface of noncancerous tissues leading to a degree
of immune tolerance, and to the fact that gangliosides do not stimulate
T-cell help. The low immunogenicity of some gangliosides is offset by
the density with which they may be expressed on the surface of
malignant cells. A further factor is the diversity of available
antigens (4), where the relative abundance of many gangliosides on the
cell surface changes during cancerous mutation with each different
ganglioside providing a possible venue for therapy (5, 6).
One such antigen is disialoganglioside GD3, which is overexpressed on
the surface of melanomas, soft tissue sarcomas, and tumors of
neuroectodermal origin, while normal tissues display little or no GD3
(7). Four forms of GD3 have been identified, which vary somewhat in the
substitution pattern of the terminal NANA residue (8, 9, 10). The best
characterized GD3 has the structure
NANA-) chimeric antibody chR24 are specific for the cell-surface
tumor antigen disialoganglioside GD3. X-ray diffraction and surface
plasmon resonance experiments have been employed to study the mechanism of "homophilic binding," in which molecules of R24 recognize and bind to other molecules of R24 though their heavy chain variable domains. R24 exhibits strong binding to liposomes containing
disialoganglioside GD3; however, the kinetics are unusual in that
saturation of binding is not observed. The binding of chR24 to
GD3-bearing liposomes is significantly weaker, suggesting that
cooperative interactions involving antibody constant regions contribute
to R24 binding of membrane-bound GD3. The crystal structures of the
Fabs from R24 and chR24 reveal the mechanism for homophilic binding and confirm that the homophilic and antigen-binding idiotopes are distinct.
The homophilic binding idiotope is formed largely by an anti-parallel
-sheet dimerization between the H2 complementarity determining
region (CDR) loops of two Fabs, while the antigen-binding idiotope is a
pocket formed by the three CDR loops on the heavy chain. The formation
of homophilic dimers requires the presence of a canonical conformation
for the H2 CDR in conjunction with participation of side chains. The
relative positions of the homophilic and antigen-binding sites allows
for a lattice of GD3-specific antibodies to be constructed, which is
stabilized by the presence of the cell membrane. This model provides
for the selective recognition by R24 of cells that overexpress GD3 on
the cell surface.
INTRODUCTION
Top
Abstract
Introduction
References
(2
8)-NANA-
(2
3)-Gal-
(1
4)-Glc-ceramide (Fig.
1). Several antibodies specific for this
antigen have been identified, with one of the most actively
investigated being the murine mAb R24. R24 has been shown to prevent
tumor development in animal models (11), as well as to display
anti-tumor activity in human clinical trials (12, 13, 14). One of the
most intriguing aspects of R24 is its ability to display homophilic
binding through a heavy chain idiotope designated IDHOM
(15), where homophilic binding is the ability of an antibody to
recognize and to bind itself. Homophilic binding is thought to amplify
the effective valence of an antibody, and so increase its avidity. R24
has also shown the ability to bind other GD3-specific antibodies in a
similar manner (16).
View larger version (8K):
[in a new window]
Fig. 1.
Schematic diagram of the most common form of
the melanoma tumor cell antigen glycosphingolipid disialoganglioside
GD3. The ceramide tail is anchored in the cell membrane, leaving
the four-sugar head group exposed to immune surveillance.
Interestingly, the antigen-binding site (designated by idiotope IDGD3) and IDHOM have been shown to be distinct, since specific mutations can disrupt homophilic binding without the loss of antigen binding (16). Homophilic binding in R24 is also distinct from the known ability of murine IgG3 to bind cooperatively to multivalent antigen through an Fc-dependent mechanism (17), because changes in residue identity in R24 near IDHOM in VH (and thus distant from the Fc) have been shown to disrupt this phenomenon (15). The avidity of a monoclonal antibody is a critical aspect of its function. Understanding the mechanism of homophilic binding in R24 will have potential impact on engineering this and other mAbs to increase their avidity without altering their specificity.
The forms of R24 that are available for study are the original murine
R24 mAb, the human-murine chimer chR24, and their respective Fabs. The
mAb chR24 is expressed from a construct in which the VL and
VH murine genes have been ligated onto the human IgG1() CL and CH genes. This form of R24 was prepared
in order to increase its effectiveness as a human therapeutic agent
while maintaining its GD3-binding properties. However, preliminary
surface plasmon resonance experiments indicated that the homophilic
binding properties of chR24 were significantly different from those of
the native R24, and a detailed comparison of the homophilic and
antigen-binding behavior using several methods was therefore necessary.
We present below the characterization of homophilic binding in R24 and
chR24 by surface plasmon resonance and x-ray crystal structure analysis.
![]() |
EXPERIMENTAL PROCEDURES |
---|
Antibody Cell Lines--
Murine anti-GD3 antibody R24 IgG3()
was obtained from hybridoma ascites of (BALB/c × C57BL)F1 mice (11). The mouse-human chimeric R24 (chR24)
was expressed from a construct consisting of the genes coding for the
R24 heavy and light variable region ligated to the respective genes
coding for human IgG1(
) constant domains, and was provided by Dr.
Alan Jarvis (Repligen Corp., Cambridge, MA).
Antibody and Fab Generation and Purification--
R24 and chR24
antibodies were purified from ascites using Protein A affinity column
chromatography (Bio-Rad). Although ascites does contain immunoglobulins
other than R24 or chR24 that will bind to a Protein A affinity column,
the purity of the mAb preparations was evident by inspection of
isoelectric focussing gels and by the subsequent crystallization of the
antigen-binding fragments. Fab fragments were prepared from the
purified antibodies by digestion with mercuripapain (Sigma, P-9886).
Mercuripapain was activated by -mercaptoethanol as described
previously (18). The mAb digest mixture contained IgG:papain in a 200:1
(w/w) ratio, and carried out in the presence of DTT (8:1 DTT:IgG molar
ratio) and brought to final volume with 20 mM sodium
acetate, pH 5.4. The digest reactions were quenched with iodoacetamide
equivalent to 2.2 mol of DTT. Time trial results established optimal
digests at 3.5 h for R24 and 2.5 h for chR24. Quenched digest
mixture was dialyzed overnight against 20 mM sodium
acetate, pH 5.4. The dialyzed digest was then filtered on a 0.22-µm
low protein-binding filter, and degassed in preparation for high
performance liquid chromatography purification (Gilson series 800 HPLC
system, Gilson Scientific Canada, Inc.). The Fab fragments were applied
to a Shodex IEC-CM825 ion exchange column (Phenomenex) in a mobile
phase of 20 mM sodium acetate, pH 5.4, and eluted against a
0-2 M sodium chloride gradient. Protein content was
assayed by absorption at 280 nm wavelength; A280/1.5 was used to calculate the Fab
concentration (in mg/ml). Absorbing fractions were assayed by
SDS-polyacrylamide gel electrophoresis (Pharmacia Phast System, 12.5%
homogeneous gels), to identify the fractions containing Fab, which were
pooled and concentrated (Centricon-10, Amicon) to between 8 and 12 mg/ml. Sodium azide was added to the concentrated Fab at 0.02% (w/v).
The concentrated Fabs were kept at 4 °C prior to crystallization trials.
Crystallization-- Primary determination of crystallization conditions for the Fabs from both R24 and chR24 were carried out using Crystal Screen I and II (Hampton Research, Laguna Hills, CA). Screens were carried out by mixing 1 µl of concentrated Fab with 1 µl of reservoir buffer and the resultant drop suspended on a plastic coverslip over 1 ml of reservoir buffer in a Linbro tissue culture 24-well plate (ICN Biomedicals, Inc., Aurora, OH). Refinement conditions were generated using reagents from Fluka Biochemika.
X-ray Crystal Structure Analysis-- Data collection was carried out at Queens University (Kingston, Ontario, Canada) using a MAR 30-cm image plate mounted on an Rigaku RU-II generator running at 6 kilowatts. Data reduction was carried out using DENZO (19). Rotation functions were carried out with MERLOT (20). Translation functions and positional refinements were carried out with XPLOR (21). Molecular modeling and fitting of electron density were carried out with FRODO (22) and SETOR (23). All molecular and electron density diagrams were generated using SETOR and SETORPLOT (23).
Surface Plasmon Resonance--
Analyses were performed using a
BIACORE 1000 biosensor system (Biacore Inc., Piscataway, NJ). Fabs used
in the experiments were from the same lot used for crystallization
studies. For antigen binding studies, ganglioside GD3 (BioCarb
Chemicals) was incorporated into liposomes also containing
dimyristoylphosphatidylcholine and Salmonella serogroup B
lipopolysaccharide as described previously (24). Se155-4, an IgG
specific for the Salmonella antigen, was immobilized on
research grade CM5 sensor chips (Biacore Inc.) and served as a capture
molecule for the GD3 liposomes (24). Liposomes (1000 RU) were captured
followed by injection of murine R24 IgG, chR24 IgG, murine R24 Fab, or
chR24 Fab over the liposome surfaces. IgG concentrations were
calculated on a per binding site basis. The liposome experiments were
carried out in 10 mM HEPES, pH 7.4, 150 mM
sodium chloride, 3.4 mM EDTA. Control surfaces were
prepared in the same way by capturing liposomes containing only
dimyristoyl- phosphatidylcholine and lipopolysaccharide. The Se155-4
IgG surfaces were regenerated by sequential injection of 1 and 4 mM sodium taurodeoxycholate in 10 mM sodium
acetate buffer, pH 4.5, and a contact time of 3 s in each
instance. For the measurement of homophilic binding under solution-like
conditions, murine R24 IgG was immobilized on research grade CM5 sensor
chips using the amine coupling kit supplied by the instrument
manufacturer. Mouse R24 IgG was then injected over this surface using
the phosphate-buffered saline, 0.05% Tween 20, 1% bovine serum
albumin, pH 7.4, buffer employed in Ref. 15 for the measurement of
homophilic binding by ELISA. Primary sensorgram data were evaluated
using the BIAevaluation 3.0 software.
![]() |
RESULTS |
---|
Crystallization of the Fab from R24-- Small crystals were noted for Crystal Screen I solutions 33, 36, and 45, and the drop sizes were scaled up to 4 and 6 µl and refinements proceeded by varying the pH of the solution along with the concentration and composition of precipitants. Solution 33 (originally 4.0 M sodium formate) yielded crystals after a few weeks with an average size of 0.1 × 0.25 × 0.2 mm3 from a solution at pH 7.5. Solution 36 (originally 8% PEG 8000, Tris-HCl, pH 8.5) yielded crystals in a few days with an average size of 0.15 × 0.2 × 0.3 mm3 from both 8% PEG 8000 in Tris-HCl at pH 8.0 and at pH 8.5, and from 10% PEG 8000 in Tris-HCl at pH 8.0. Solution 45 (originally 18% PEG 8000, 0.1 M sodium cacodylate, pH 6.5, 0.2 M zinc acetate) yielded crystals with an average size of 0.2 × 0.2 × 0.25 mm3 from this solution and from 23% PEG 8000, 0.2 M zinc acetate in 0.1 M sodium cacodylate, pH 7.0, from which grew the crystal that gave the highest resolution data.
Crystallization of the Fab from chR24-- Solution 45 from Crystal Screen I (0.2 M zinc acetate, 0.1 M sodium cacodylate, pH 6.5, 18% PEG 8000) yielded small crystals after 1 week. Initial refinements were carried out at 4 °C and 20 °C, and consisted of combinations of pH from 6 to 8.5 in increments of 0.5, PEG 4000, 8000, and 10,000, at concentrations of 13, 18, and 23%, with the concentrations of zinc acetate and sodium cacodylate kept constant. The crystal selected for data collection was obtained by macroseeding a drop of 0.2 M zinc acetate and 0.1 M sodium cacodylate at pH 7.0 with 23% PEG 8000.
X-ray Diffraction Data Collection--
For crystals of the Fab
from R24, 58,762 reflections were measured yielding 26,595 unique
reflections to 3.1-Å resolution (92% complete) with
Rsym = 0.075. The Fab was found to crystallize in the monoclinic space group P21 with cell
dimensions a = 139.2, b = 82.1, c = 73.6 Å, and = 94.1°. For crystals of the Fab
from chR24, 79,380 reflections were measured yielding 18,143 unique reflections to 2.5-Å resolution (94% complete) with
Rsym = 0.050. The protein was found to
crystallize in the monoclinic space group C2 with cell
dimensions a = 146.8, b = 56.0, c = 80.0 Å, and
= 119.2°.
Structure Solution--
The structure of the Fab from murine R24
was solved by molecular replacement methods using the known structure
of the Fab from YsT9.1 specific to Brucella abortus as a
trial structure (25, 26). The volume of the unit cell indicated that it
probably contained three molecules of R24 Fab per asymmetric unit;
however, the rotation solution revealed at best only two clear
solutions for the model of the constant domain dimer no matter what
combination of resolution range, cutoff, and integration radius was
used. The best results were found using all data with an input
resolution range of 6 to 4 Å and integration radius of 23 Å. The
translation solution for the two fragments (including the
cross-translation function) was determined with the aid of the
Patterson correlation (PC) refinement function in XPLOR. The two
variable domain dimers were positioned by executing individual
elbow-angle searches using PC refinement about the corresponding
constant domain dimers. Several rounds of rigid-body refinement with
all data from 6 to 4 Å resolution with I
3(
)I were carried out using first the entire Fab as a
rigid body, then the intact variable and constant domains, and finally
the heavy and light chains of each of the two domains. This resulted in
an R-factor of 0.26. The sequence corresponding to R24 was
then imposed on the model using SETOR. Hendrickson-Konnert refinements
were carried out, alternating with rounds of manual intervention to
position the CDRs and other residues. Due to the limited resolution of
the data (3.1 Å), all data (i.e. no
cutoff) was used
for the final cycles of refinement, no water molecules were added to
the trial structure, and individual temperature factors were not
allowed to refine independently. As the nature of the interaction
between the two molecules in the asymmetric unit was the object of the
analysis, the observed non-crystallographic two-fold symmetry axis was
not applied during refinement. The R-factor converged at
0.245 for all data. The final refined overall isotropic temperature
factor was 28.4 Å2, which is somewhat lower than the value
of 35.3 Å2 obtained from a Wilson plot. An
Rfree was calculated in a separate refinement to
be 0.334, which indicated that the refined model was somewhat
underdetermined at 3.1-Å resolution.
The structure of the Fab from chR24 was determined using the structure
for R24 as a trial structure. A search using XPLOR clearly revealed the
orientation and position of the variable domain dimer of chR24 (which
has the same sequence as the corresponding domain dimer from R24). An
elbow-angle search combined with PC refinement in XPLOR revealed the
position of the constant domain dimer. The sequence of the constant
domains of both the heavy and light chains were changed to correspond
to human IgG1() using SETOR. Refinement proceeded as for R24;
however, there were enough data for individual isotropic temperature
factors to be refined and for the positions of several water molecules
to be assigned. The structure was refined to a final resolution of 2.5 Å, with a final R-factor of 0.162 for all data with
I
2
(i), with 74 water molecules.
Quality of the X-ray Crystal Structures-- The standard deviation of the distribution of the bond lengths and bond angles about ideal values after the final cycles of refinement was found to be 0.022 Å and 3.8°, and 0.015 Å and 1.35° for R24 and chR24, respectively. The Ramachandran plots (not shown) for both structures reveal that few non-glycine residues lie outside the allowed regions. The electron density is well defined over the course of most of the polypeptide chain, even for the 3.1 Å resolution R24 structure. Interestingly, it was the lower resolution R24 structure that was solved first, where the trial phases from the molecular replacement solution were sufficient to trace the entire polypeptide chain. The conformations of all six CDRs in both structures lie in well defined electron density, and the H2 CDR loops from both structures are presented in Fig. 2.
|
Comparison of R24 and chR24 Structures--
The positions of all
atoms in the two molecules in the asymmetric unit for R24 were refined
independently. Although the R24 Fab crystals scattered to only 3.1 Å resolution, a least-squares superposition of the -carbon atoms
applied separately to the Fv and CH1-CL domain
dimers of the two molecules shows no significant difference in the
positions of main chain atoms, and small changes in conformation of a
few side chains. The "elbow" angles (defined as the angle subtended
by the pseudo two-fold axes of the variable and constant domains of an
Fab) are not significantly different between R24 and chR24 at 166.7 and
167.3°. A least-squares superposition of the
-carbon atoms of the
Fvs of chR24 onto either Fv from R24 gives a root-mean-square deviation
of less than 1.0 Å, and shows that the humanization process has had no
significant effect on the conformation of the Fv.
Characterization of Homophilic Binding--
Surface plasmon
resonance experiments showed that both murine R24 IgG and chimeric
chR24 IgG bound to GD3-bearing liposome surfaces, but with strikingly
different affinities (Fig. 3,
a and b). At concentrations of 2 µM, R24 gave a response of 1700 RU at the end of the
injection whereas chimeric R24 IgG gave a response of only 35 RU under
the same conditions. The most interesting feature of the sensorgrams is
the apparent lack of saturation associated with R24 binding to
GD3-bearing liposomes. This is characteristic of homophilic binding,
and is similar to the results reported previously for the binding of
R24 IgG to human melanoma cell lines expressing GD3 (15). The binding
kinetics were observed to be complex at all concentrations of IgG.
Distinctly biphasic behavior was evident at higher R24 IgG
concentrations of 1 and 2 µM. A "fast-binding" phase
is observed to dominate in the first few seconds, accounting for
approximately 70% of the bound antibody, followed by a
"slow-binding" phase, which gradually increases the amount of bound
antibody over a period of several minutes. The rapid initial
association may reflect both GD3 antibody recognition and homophilic
binding, with the slower association attributable solely to homophilic
interactions. The kinetics are simpler at lower concentrations of R24
(Fig. 3a, inset) where biphasic behavior is not
observed; however, even these data do not fit to a simple bivalent
analyte model. The data collected at lower IgG concentrations do fit
well to a simple one-to-one interaction model, which gives an
association rate constant of 1.3 × 105
M1 s
1 and a dissociation rate
constant of 2.3 × 10
3 s
1
(KD = 18 nM). It was not possible to
derive similar rate constants for chR24 because complex biphasic
binding is observed at all concentrations (Fig. 3b).
|
Scatchard analysis of equilibrium binding data obtained for the binding of murine R24 Fab to liposomes containing 10% GD3 is presented in Fig. 4. The plot of bound versus bound/free R24 shows that homophilic binding was occurring, as a straight line with a positive slope indicates that the proportion of available Fab that bound to liposomes increases with increasing Fab concentration. The extent of Fab dimerization resulting from homophilic binding would increase with concentration, and the resulting higher proportion of bivalent Fab would be manifested as increased functional affinity. Equilibrium binding was achieved very rapidly at the Fab concentrations used, and the dissociation of Fab from the liposomes was too rapid to allow the derivation of kinetic constants. These results also agree with reported experiments binding R24 IgG to human melanoma cell lines (15). Scatchard analysis of the R24 IgG data did not give a straight line and confirmed the non-saturable nature of analyte binding. It is not surprising that the IgG data was not as easily analyzed as that of the Fab, since bivalency adds complexity to the binding reaction.
|
The affinity of chR24 Fab for GD3-bearing liposomes was significantly weaker than that of the murine Fab. A concentration of 80 µM gave an equilibrium response of 160 RU; this is the same response obtained with murine R24 at a concentration of 6 µM. A Scatchard plot of the equilibrium binding data obtained with chR24 Fab was consistent with non-saturable binding in that a line with a slope of zero was obtained instead of the negative slope expected for a simple one-to-one interaction.
By SPR analysis, R24 homophilic binding was only observed in the
presence of GD3-bearing liposomes. When R24 IgG was covalently coupled
to the dextran matrix of the sensor chips to give IgG surface densities
of 12,500 RU, which corresponds to approximately 12.5 ng/mm2, injected R24 IgG at concentrations as high as 1 µM, did not bind to these surfaces, showing that no
significant level of homophilic binding occurs in the absence of
liposomes containing GD3. This is in contrast to ELISA data, which
showed significant binding of biotinylated R24 IgG, over a
concentration of 1-100 µg/ml (0.01-1.3 µM), to plates
coated with R24 IgG at a concentration of 5 µg/ml (15). Coating the
ELISA plate at this antibody concentration would give an IgG surface
density of 3 ng/mm2 if 100% of the antibody adsorbed to
the plate. The discrepancy between the ELISA data and the SPR results
obtained using immobilized IgG may reflect the fact that IgG would
exist under solution-like conditions when linked to dextran chains on
sensor chips, whereas adsorption to microtiter plates would present the
IgG in a rigid two-dimensional environment more closely resembling cell
membrane and liposome surfaces.
![]() |
DISCUSSION |
---|
Comparison of the Fabs from R24 and chR24--
The chR24 mAb is a
chimer, with the Fv domain sharing sequence identity with the Fv from
R24, and with the CH1-CL domain dimer corresponding to human IgG1(). The chR24 mAb was created in order to
preserve the GD3 recognition site while lowering in humans the degree
of adverse immune reaction against a murine mAb. This is the first
report of the three-dimensional structures of both the uncomplexed
murine Fab and mouse-human Fv- CH1-CL chimer. The two published reports of chimeric Fab structures describe, respectively, the structure of a native murine Fab (27), and a
comparison of the complexed and uncomplexed forms of a chimeric Fab
(28). The interface between the Fv and CH1-CL
domain dimers has been implicated in transmitting an allosteric
response to binding from the Fv to the CH1-CL
domain dimer of the Fab upon complexation with antigen (29). The
transition from murine to human amino acids occurs after residues
Leu-108 and His-123, which are both located in hinge regions of the
Fab. The elbow angle for chR24 is comparable to R24, and the constant
domains of chR24 occupy a position and orientation that is close to the
native murine form.
GD3 Binding Site--
R24 and chR24 antibodies are specific for
the ganglioside GD3 (8, 9, 10), of which the most common form consists
of NANA-(2
8)-NANA-
(2
3)-Gal-
(1
4)-Glc-ceramide (Fig.
1). Examination of the binding surface of the Fvs shows that the three
CDRs of the VH cooperate to form a deep pocket which serves
to recognize and to bind the antigen (Fig.
5). The pocket is lined by polar amino
acids (serine, threonine, histidine) as well as some tyrosine residues
and has a well defined mouth or lip. The pocket measures approximately
8.5 × 12 × 8 Å3, which allows it to completely
accommodate the terminal sialic acid residue on GD3. The substitution
of the CL-CH1 domains has had little effect on
the conformation of residues lining the binding pocket between the R24
and chR24 structures, as a superposition of the residues lining the
binding pocket of chR24 shows a root-mean-square deviation of only 0.56 Å.
|
Characterization of Homophilic Binding--
The binding to
liposomal GD3 of R24 and chR24 IgG and Fab was investigated in order to
better understand the factors contributing to their different binding
profiles, especially given that mutations in VH have been
shown to disrupt the homophilic binding phenomenon in chR24 (16).
Murine R24 IgG displays strong binding to GD3-bearing liposomes, which
can be attributed to homophilic binding between molecules of R24 and
the formation of a multivalent antibody lattice. The reduced levels of
binding displayed by the chimeric chR24 IgG reveal the heavy
participation of the constant regions of the murine R24 IgG in
homophilic binding. As the structures of the Fvs of R24 and chR24 have
been found in this study not to be significantly different, the
difference in activity observed between R24 and chR24 must stem from
the change in constant regions from murine IgG3() to human
IgG1(
). Similar differences in antibody affinities that accompany
changes in constant region subclass have been reported (30), and
attributed largely to the ability of murine IgG3 antibodies to form
multivalent lattices. However, the chimeric chR24 antibody with its
human IgG1 heavy chain still displays some homophilic binding.
Loss of homophilic binding in chR24 had been demonstrated previously to occur in certain VH mutants, leading to the assignment of a specific idiotope for homophilic binding designated IDHOM (16). The levels of homophilic binding that are displayed by chR24 IgG can be attributed directly to cooperative VH-VH interactions through IDHOM.
Interestingly, neither the R24 or chR24 IgGs display a significant level of homophilic binding under solution conditions, indicating that there is a requirement for the GD3 antigen and/or a membrane-like surface to induce this phenomenon. Thus, stable binding of GD3 by R24 is a cooperative process requiring VH-VH interactions (which can be disrupted by single point mutations), Fc-Fc interactions (apparent from the difference between R24 Fab and IgG binding), and the immobilized antigen presented on a membrane. The formation of antibody lattices on cell surfaces would obviously also be influenced by GD3 concentration in the membrane.
A significant difference in binding was also observed between the R24 and chR24 Fabs, where the Fab from R24 again showed superior homophilic binding ability. Like their parent molecule, the Fabs from murine IgG3 antibodies display the ability to form multivalent species through their constant regions. This behavior of murine R24 manifested itself during crystallization trials, where the protein was found to be less soluble than the Fab from chR24 (2.5 versus 12 mg/ml).
Fab Dimerization and the Assignment of
IDHOM--
Structural studies of the Fabs corresponding to
R24 and chR24 were undertaken to allow a characterization of the
interactions of the variable domains in the crystalline state in the
absence of the Fc. The Fabs from both R24 and chR24 are indeed observed to form dimers in the crystal lattice. R24 crystallizes in space group
P21, where the dimers are composed of two
independent molecules in the asymmetric unit related by a
non-crystallographic two-fold axis of symmetry. Fabs corresponding to
chR24 crystallize in space group C2, where the dimers are
composed of two molecules related by a true crystallographic two-fold
axis. Despite the difference in packing arrangement, the dimerization
interactions exhibited by R24 and chR24 are almost identical. A
schematic and wire-frame model of the contact area of dimerization for
chR24 in the solid state is given in Fig.
6. The dimerization proceeds though a
continuation of the anti-parallel -sheet structure of the H2 loop.
Table I details the four observed
intermolecular main chain hydrogen bonds that are formed, and lists
several side chain hydrogen bonds and a hydrophobic interaction that
contribute to the stability of the dimer. The dimer displays a degree
of flexibility in that R24 and chR24 display slightly different
dimerization geometry. The angles between the pseudo two-fold axis of
the VL and VH are 167° in R24 and 155° in
chR24. Examination of the rotational freedom about the
-sheet
interface shows that the dimer can flex through an angle of
approximately 20° without generating significant steric interactions,
or disrupting the intermolecular
-sheet interaction.
|
|
The remaining intermolecular contacts made by the Fab dimers in the crystal lattice are different in R24 and chR24, where R24 shows contacts from all various of heavy and light chains, while chR24 displays contacts primarily from the VL domains to the CH1 domains of neighboring Fabs in the crystal lattice.
Fab Dimerization and the Formation of an Antibody Network--
An
-carbon backbone of the chR24 dimer as it occurs in the crystal
structure is presented in Fig. 7. The
orientation of the two-fold axis relating the Fvs is such that the N
terminus of the heavy chains of the two Fabs in the dimer can approach
the cell surface close enough to simultaneously bind the terminal residue of GD3 in the antigen-binding pocket while in the dimerized state.
|
This conformation of the dimer in combination with the surface plasmon resonance studies leads to a model for the formation of an antibody network. Fig. 8 depicts a tumor cell expressing ganglioside GD3 on its surface and the resulting antibody network in which molecules of chR24 are bound to the surface antigen and to each other through IDHOM. The necessary proximity of the dimer Fv surfaces to the membrane serves to immobilize and stabilize the dimer, and allows for possible interactions between the membrane and the Fv. This model explains the apparent requirement for the presence of the membrane, and why R24 is a relatively labile dimer in solution but forms strong homophilic networks while bound to a GD3-bearing surface. The large number of cooperative interactions formed by the mAb lead to stabilization of the dimer and to the formation of larger antibody networks. The cooperative network of dimers would give the antibody a high potential valence, leading to a low off rate.
|
Comparison with Reported Structures of Antibody Antigen-binding
Sites--
Of the Fab and Fv structures deposited in the Protein Data
Bank (31) that were accessible to date, only one other structure (the
Fab from mAb HIL; code 8FAB) displays dimerization through any part of
the Fv site. The dimerization of HIL occurs though a similar pairing of
the H2 loop main chain and side chain interactions as found for R24 and
chR24. Four major canonical conformation have been associated with the
H2 loop (32), which are represented schematically in Fig.
9. R24, chR24, and HIL all have H2 loops that belong to type 3, which is the only conformation in which all the
main chain hydrogen bonding groups are positioned to form an
anti-parallel -sheet interaction. The formation of main chain hydrogen-bonded pairs in the crystal lattice has not been observed for
type 1, 2, and 4 H2 loops.
|
There are at least 12 other Fab or Fv structures that display type 3 H2 loop conformations, yet only R24 chR24 and HIL display dimerization through the H2 loop. This indicates that not only must the H2 canonical form be present on the Fab, it must also possess a primary structure that is capable of forming appropriate side chain interactions. Evidence for this point can be seen in studies of homophilic binding for chR24 mutants (16), where changes in residue identity at or near IDHOM almost always disrupt homophilic binding.
Interestingly, -sheet dimerization of Fabs is not restricted to the
Fv. Three other structures (7FAB (33), 1DBJ (34), 1GGB (35)) show
-sheet coupling through their CH1 domains, and well away
from the antigen-binding site. The parent antibodies of these three
structures do not share a common heavy chain, being, respectively,
murine IgG1, murine IgG2a, and human IgG1. Homophilic binding through
the first constant region can serve a purpose similar to binding
through the Fv, but cannot be properly classed as an idiotope.
Significance of Homophilic Binding in Cancer Therapy--
Direct
comparisons in mouse models of the therapeutic value of high and low
affinity mAbs suggest that higher affinity antibodies are more
effective in cancer treatment (36, 37). The changes observed in R24 and
chR24 antigen binding that occur with the changes in their ability to
display homophilic binding demonstrate that this class of phenomenon
should be explored further as a means of increasing effective antigen
affinity without altering specificity.
![]() |
ACKNOWLEDGEMENT |
---|
We thank Dr. Z. C. Jia at Queens University, Kingston, Ontario, Canada, for access to his data collection equipment and assistance with the data collection.
![]() |
FOOTNOTES |
---|
* This work is funded by a grant from the Medical Research Council of Canada (to S. V. E.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The atomic coordinates and structure factors (codes 1R24/R1R24SF for R24 and 1BZ7/R1BZ7SF for chR24) have been deposited in the Protein Data Bank, Brookhaven National Laboratory, Upton, NY.
To whom correspondence should be addressed: Dept. of
Biochemistry, University of Ottawa, 451 Smyth, Ottawa, Ontario K1H
8M5, Canada. Tel.: 613-562-5800 (ext. 8437); Fax: 613-562-5440; E-mail: evans{at}uottawa.ca.
![]() |
ABBREVIATIONS |
---|
The abbreviations used are: mAb, monoclonal antibody; CDR, complementarity determining region; CH1, first constant region of an antibody heavy chain; CL, constant region of an antibody light chain; DTT, dithiothreitol; ELISA, enzyme-linked immunosorbent assay; Fab, fragment comprising the antibody Fv and first constant region domain dimer; Fc, carboxyl-terminal fragment comprising the second and third constant regions of an IgG antibody; Fv, fragment comprising the antibody variable domain dimer; Gal, galactose; GD3, disialoganglioside GD3; Glc, glucose; ID, idiotope; NANA, N-acetylneuraminic acid; PC, Patterson correlation; PEG, polyethylene glycol; RU, resonance unit(s); VH, heavy chain of an antibody variable region; VL, light chain of an antibody variable region.
![]() |
REFERENCES |
---|
![]() ![]() ![]() ![]() |
---|