Topology of the Stable Serpin-Protease Complexes Revealed by an Autoantibody That Fails to React with the Monomeric Conformers of Antithrombin*

Véronique PicardDagger , Pierre-Emmanuel MarqueDagger , Francis Paolucci§, Martine AiachDagger , and Bernard F. Le BonniecDagger

From Dagger  INSERM, Unité 428, Université Paris V, 75270 Paris Cedex 06 and § Sanofi Research, 34000 Montpellier, France

    ABSTRACT
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Abstract
Introduction
References

Solving the structure of the stable complex between a serine protease inhibitor (serpin) and its target has been a long standing goal. We describe herein the characterization of a monoclonal antibody that selectively recognizes antithrombin in complex with either thrombin, factor Xa, or a synthetic peptide corresponding to residues P14 to P9 of the serpin's reactive center loop (RCL, ultimately cleaved between the P1 and P'1 residues). Accordingly, this antibody reacts with none of the monomeric conformers of antithrombin (native, latent, and RCL-cleaved) and does not recognize heparin-activated antithrombin or antithrombin bound to a non-catalytic mutant of thrombin (S195A, in which the serine of the charge stabilizing system has been swapped for alanine). The neoepitope encompasses the motif DAFHK, located in native antithrombin on strand 4 of beta -sheet A, which becomes strand 5 of beta -sheet A in the RCL-cleaved and latent conformers. The inferences on the structure of the antithrombin-protease stable complex are that either a major remodeling of antithrombin accompanies the final elaboration of the complex or that, within the complex, at the most residues P14 to P6 of the RCL are inserted into beta -sheet A. These conclusions limit drastically the possible locations of the defeated protease within the complex.

    INTRODUCTION
Top
Abstract
Introduction
References

Serine protease inhibitors (serpins)1 are mainly composed of three beta -sheets (A, B, and C) united by nine alpha -helices (A-I); indeed, many are inhibitors that neutralize their target(s) by forming a, stoichiometric, stable complex (1-3). Formation of the stable complex involves the charge stabilizing system of the target protease (4-8) and a surface loop of the inhibitor called the reactive center loop (RCL). The RCL connects strand 4 of beta -sheet A to strand 1 of beta -sheet C; it is exposed to solvent in the inhibitory serpins. By analogy with protease substrates, the 20 amino acids constituting the RCL are numbered Pn- ... -P1-P'1- ... -P'n, where P1-P'1 is ultimately cleaved. The mechanism of protease inhibition involves multiple steps that initiate by the formation of a reversible association, converting to a stable complex, ultimately split into regenerated enzyme and RCL-cleaved (consumed) serpin (9-12). The RCL sustains a variety of conformations (13-16). In antithrombin (AT) that is heparin-activated (17, 18) and other inhibitory serpins such as alpha 1-antitrypsin (also called alpha 1-proteinase inhibitor; 19-21) or alpha 1-antichymotrypsin (22), the RCL is wholly exposed, whereas in the AT monomer, residue P14 of the RCL disrupts beta -sheet A (23-25). In latent AT, an intact but non-inhibitory conformer (25), and in latent type-1 plasminogen activator inhibitor (16), residues P14 to P3 of the RCL are completely inserted into beta -sheet A, constituting an additional, sixth, strand. The same conversion from a five- to six-stranded beta -sheet A occurs in the inhibitory serpins, following cleavage of the RCL (14, 26).

To date, no x-ray analysis of a serpin-protease complex has been reported; thus, its structure remains largely hypothetical. Based on functional studies of serpin variants and immunochemical investigations, a number of reports nevertheless suggest that, in the stable complex, the RCL is inserted into beta -sheet A. Antibodies have been characterized that fail to react with native serpin, but recognize binary complexes with a synthetic tetradecapeptide corresponding to residues P14 to P1 of the RCL, as well as consumed inhibitors (27-33). Thus, antibodies revealed that insertion of the RCL in beta -sheet A exposes neoepitopes that are not present in the intact inhibitor. The same neoepitopes being exposed in stable serpin-protease complexes led to the conclusion that, during trapping of the protease, the RCL inserts at least in part into beta -sheet A. Convincing evidence also suggests that the protease translocates away from the site at which initial attack occurs. Wright and Scarsdale (34) even proposed that the enzyme ends in a location almost opposite to that of the RCL in intact serpins, i.e. that, in the stable serpin-protease complexes, beta -sheet A is a six-stranded sheet. Stratikos and Gettins (35) demonstrated that, at least 21 Å separates position of the protease in the initial (reversible) Michaelis complex from that in the (virtually irreversible) final complex. Modeling considerations suggest a range of plausible stable structures: from full insertion of the RCL with upside-down translocation of the defeated protease, to limited RCL insertion (up to the P9 residue) with concomitant stacking of the target on the F-helix. Following cross linking experiments with stable complexes of type-1 plasminogen activator inhibitor, Wilczynska et al. (36) favored stacking on the F-helix, and partial rather than complete insertion of the cleaved RCL.

We report herein characterization of a monoclonal antibody (12A5) that provides definite insight into the structure of the stable AT-protease complex; unless AT was unfolded, the antibody failed to react with any AT monomers, while endorsing stable complexes with thrombin or factor Xa and binary complex with a short peptide derived from the RCL. The unexpected location of the neoepitope, on the distal part of beta -sheet A, has several fundamental implications. The conformations of beta -sheet A and/or of the F-helix in the stable complex must simultaneously differ from the five- and six-stranded structures of native and RCL-cleaved AT, respectively. Steric hindrance considerations also limit drastically the possible topologies of the complex.

    EXPERIMENTAL PROCEDURES

Materials-- Human thrombin and its S195A variant were prepared as described previously (8). Factor Xa was purchased from ERL (South Bend, IL). Porcine pancreatic elastase (type IV) and bovine serum albumin (BSA, protease-free) were from Sigma (St-Quentin Fallavier, France), as well as mouse AT and bovine, sheep, porcine, rabbit, and chicken plasma. Standard, unfractionated heparin (Heparin Choay) and pentasaccharide with high affinity for AT (M, 1714) were from Sanofi-Winthrop (Gentilly, France). Peptide P14-P9 derived from the RCL of AT (Ac-SEAAAS) was synthesized by Altergen (Schiltigheim, France).

Preparation and Characterization of the Various AT Conformers-- Human AT was purified from citrated frozen plasma essentially according to McKay (37) by affinity chromatography on heparin-Sepharose (Pharmacia, St-Quentin-en-Yvelines, France), followed by anion-exchange chromatography on a Mono-Q column (Pharmacia). AT cleaved by porcine pancreatic elastase was prepared by incubating AT (5 µM) with 50 nM enzyme for 4 h at 37 °C in 50 mM Tris-HCl, pH 8.5, containing 0.15 M NaCl and 0.1% polyethylene glycol (Mr 8000; w/v). The resulting C-terminal fragment was isolated by transfer onto polyvinylidene difluoride membrane after denaturing polyacrylamide gel electrophoresis (15% acrylamide), and analyzed by N-terminal sequencing (Biotechnology Department, Institut Pasteur, Paris, France). Two sites of cleavage were identified: Val389-Ile390 and Ile390-Ala391, corresponding to the P5-P4 and P4-P3 residues of the RCL, respectively. Identical cleavages are obtained following incubation of AT with human neutrophil elastase (38). Thrombin-cleaved AT was prepared by incubating AT (7 µM) with thrombin (0.1 µM) for 3 h at 37 °C in 50 mM Tris-HCl, pH 7.5, containing 0.15 M NaCl and trace amounts of SDS (0.02%, w/v; Refs. 39 and 40). Thrombin was neutralized by the addition of 1 mM phenylmethylsulfonyl fluoride (Sigma). Traces of SDS and excess phenylmethylsulfonyl fluoride were removed by extensive dialysis against 50 mM Tris-HCl, pH 7.5, containing 0.15 M NaCl. Denaturing polyacrylamide gel electrophoresis (Fig. 1) suggested that in these conditions AT was fully cleaved by thrombin, at a single site. In addition to the N terminus of AT, N-terminal sequencing of the reaction mixture revealed a single sequence starting with the P'1 residue of the RCL, indicating that thrombin cleaved a single bond in AT, between the P1 and P'1 residues of the RCL. AT cleaved by porcine pancreatic elastase or by thrombin were indistinguishable by polyacrylamide gel electrophoresis analysis (Fig. 1) and had a similar affinity for heparin (i.e. reduced compared with native AT); both eluted at about 0.3 M NaCl on heparin-Sepharose column. Latent AT was prepared according to Wardell et al. (41). Briefly, AT (1.7 µM) was incubated for 18 h at 60 °C in 10 mM Tris-HCl, pH 7.5, containing 0.35 M sodium citrate. Following incubation, the mixture was dialyzed against 10 mM Tris-HCl, pH 7.5, and latent AT was recovered by heparin-Sepharose chromatography and anion-exchange chromatography on Mono Q column, both developed with a linear gradient from 0.0 to 0.5 M NaCl; latent AT eluted at 0.3 and 0.2 M NaCl, respectively. The concentration of all AT monomers was estimated assuming an absorption coefficient of 3.8 × 104 M-1 cm-1 at 280 nm (epsilon %=6.5 cm-1). Stable complexes of AT with thrombin or factor Xa were prepared by incubating AT (5 µM) for 2 h at room temperature with thrombin (5 µM) or for 4 h with factor Xa (5 µM) in 50 mM Tris-HCl, pH 7.5, containing 0.15 M NaCl and 0.1% polyethylene glycol (Mr 8000; w/v). Virtually no monomer of AT (either native or RCL-cleaved) could be seen by polyacrylamide gel electrophoresis analysis in the presence of SDS (Fig. 1); therefore, concentrations of the complexes were assumed to be that of the added AT (5 µM). Reversible complex of AT with S195A thrombin was prepared by mixing AT (2 µM) with S195A thrombin (2 µM) in the presence of heparin (1 unit/ml); association was presumed to be almost instantaneous (8), and concentration of the reversible complex was assumed to be that of the added AT. Formation of a binary complex with the P14-P9 peptide derived from the RCL was carried out according to Schulze et al. (28) and Chang et al. (42), by incubation of AT (20 µM) and peptide (2 mM) in 50 mM Tris, pH 8.0, containing 50 mM NaCl. Binary complex was used immediately after a 60-min incubation at 37 °C: when less than 50% AT formed a complex and no polymerization of AT had yet occurred (Fig. 1). Concentration of the binary complex was assumed to be 40% that of the total amount of AT.


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Fig. 1.   Polyacrylamide gel electrophoresis of the various AT conformers used. Left panel, electrophoresis in denaturing conditions of: native AT (lane 1), AT cleaved by porcine pancreatic elastase (lane 2), AT cleaved by thrombin (lane 3), thrombin (lane 4), complex of AT with thrombin (lane 5), factor Xa (lane 6), and complex of AT with factor Xa (lane 7). Right panel, electrophoresis in non-denaturing conditions of: native AT (lane 1), latent AT (lane 2), and AT partially in complex with the P14-P9 peptide derived from the RCL (lane 3).

Preparation and Characterization of the Monoclonal Antibodies-- Murine monoclonal antibodies directed against human AT were selected, isolated and characterized by standard procedures (43). Four 6-week-old BALB/c females were immunized by subcutaneous injection of 20 µg of AT in complete Freund's adjuvant followed 3 weeks later by a further subcutaneous injection of 20 µg of AT in incomplete Freund's adjuvant. One hundred days later, mouse received 8 µg of AT subcutaneously and 12 µg of AT intravenously. One day prior to the AT injection, and for 5 days, mice also received a daily intraperitoneal injection of 20 µg of pentasaccharide. Three days after the final injection, spleen cells were fused with the mouse myeloma cell line P3-X63-Ag.8.653 by using 50% polyethylene glycol 1540, and cultured in hypoxanthine/aminopterin/thymidine media. Positive clones were detected by enzyme-linked immunosorbent assay (ELISA); Maxisorp microplates from Nunc (Polylabo, Strasbourg, France) were coated overnight at 4 °C with 5 µg/ml AT in 0.1 M Na2HCO3/NaH2CO3, pH 9.5, and washed three times in 20 mM Na2HPO4/NaH2PO4, pH 7.4, containing 0.15 M NaCl (PBS) and 0.5% Tween 20 (v/v). Residual sites were blocked for 1 h with 1 mg/ml BSA in PBS, and microplates were washed as above. Each hybridoma supernatant to be tested was added to one of the wells of the microplate and allowed to bind for 1 h at room temperature. After washing, a peroxidase-conjugated goat anti-mouse antibody (Bio-Rad, Ivry-sur-Seine, France) was added and allowed to bind for 1 h at room temperature. Following a final wash, ELISA was developed by adding 150 µl of orthophenylenediamine (0.5 mg/ml; Bio-Rad) in 0.1 M sodium citrate, pH 5.5, containing 0.3% H2O2 (v/v). The reaction was stopped after 30 min by the addition of 50 µl of H2SO4 (12.5%, v/v), and the absorbance at 490 nm was recorded. Selected clones were grown in pristane-primed BALB/c mice, and antibodies purified from the ascitic fluid by affinity chromatography on protein A-Sepharose (Pharmacia). IgG appeared pure by denaturing polyacrylamide gel electrophoresis analysis and were stored at -80 °C after dialysis against PBS. The subclass was determined with a commercial kit (Amersham, Les Ulis, France): monoclonal 12A5 described in this study was typed as IgG2a.

Determination of the AT Conformers Recognized-- To identify the conformers of AT recognized by the monoclonal antibodies, a sandwich ELISA was designed in which microplates were coated with purified IgG (5 µg/ml). Each AT conformer, diluted in PBS containing 0.5% Tween 20 (v/v) and 1 mg/ml BSA, was added to one of the wells of the microplate and allowed to bind for 2 h at room temperature. Sandwich ELISA was developed with a peroxidase-conjugated goat IgG directed against human AT (Sanofi Research, Montpellier, France). In assays with S195A-AT reversible complexes, 1 unit/ml heparin was present during the binding step, and all buffers included 0.1 unit/ml heparin to prevent release throughout the washing steps. Ability of the S195A-AT complexes to be retained on a microtiter plate was assessed by the use of a commercial kit designed to detect plasma thrombin-AT complexes (Enzygnost TAT micro; Behring, Marburg, Germany). A microplate, coated with a polyclonal antibody directed against thrombin was incubated with various amounts of S195A-AT complexes and developed with a polyclonal antibody directed against AT, according to the manufacturer's instructions except that 0.1 unit/ml heparin was added in all buffers.

Epitope Mapping-- Customized AT peptide sets were prepared by Chiron Mimotopes Peptide Systems (Clayton, Victoria, Australia) as biotinylated peptides linked to a cleavable polyethylene pin (44). A four-amino acid spacer (GSGS) separated the N terminus of each peptide from the pin, except for the peptide encompassing the first 14 amino acids of AT where the spacer was coupled to the C terminus. Binding assays were performed according to the supplier's instructions. Briefly, 100 µl of streptavidin (5 µg/ml in water) was added to each well of a microplate and evaporated to dryness at 37 °C. Biotinylated peptides (about 1 µM in PBS containing 1 mg/ml BSA) were added and incubated for 1 h at room temperature. Monoclonal antibody (0.1 µg/ml) in PBS containing 0.5% Tween 20 (v/v) and 1 mg/ml BSA was incubated for 2 h at room temperature. Washing and development were otherwise performed as described above for the ELISA, using the peroxidase-conjugated goat anti-mouse IgG.

Immunoblotting-- Immunoblotting of the various AT conformers (after polyacrylamide gel electrophoresis in denaturing conditions) was performed on nitrocellulose membrane (Bio-Rad) essentially as described previously (45). Incubation and washing were all completed at room temperature in 50 mM Tris-HCl, pH 8.0, containing 0.15 M NaCl and 0.5% Tween 20 (v/v). The membrane was saturated with nonfat dry milk (3% w/v), washed, and incubated for 1 h with monoclonal antibody 12A5 (0.5 µg/ml). After washing, the membrane was incubated for 1 h with an alkaline phosphatase-conjugated goat anti-mouse IgG (Bio-Rad). The immunoblot was further washed, and developed by adding a phosphatase substrate solution consisting of 0.15 mg/ml 5-bromo-4-chloro-3-indolyl phosphate and 0.3 mg/ml nitro blue tetrazolium in 0.1 M Tris, pH 9.5, containing 0.5 mM MgCl2.

Inhibition Kinetics-- The influence of monoclonal antibody 12A5 on thrombin and factor Xa inhibition by AT was studied in the absence and in the presence of heparin, essentially as described previously (46, 47). Assays were performed at 37 °C in 50 mM Tris-HCl, pH 7.6, containing 0.15 M NaCl, and 0.1% polyethylene glycol (Mr 8000; w/v); 1 mg/ml BSA was included in the assay of factor Xa inhibition. Without heparin, estimates of the association rate constant (kon), in the presence of 1 µM 12A5 (or control IgG), were obtained from kinetic experiments performed in microplates, under pseudo first-order conditions (20-100 nM AT with 2 nM thrombin, and 150-300 nM AT with 15 nM factor Xa). First order rate constants were estimated by non-linear regression analysis of the residual activities versus time (up to 90 min), and kon values deduced from the linear plot of the first order rate constants as a function of AT. The kon values in presence of heparin (1.4 units/ml), were estimated by analysis of data from progress-curve kinetics also completed in pseudo first-order conditions (0.2-0.6 nM AT with 10 pM thrombin, and 1-2 nM AT with 100 pM factor Xa). Inhibition of thrombin was initiated by its addition to a microtiter well containing 0.2 µM 12A5 (or control IgG), AT at various concentration, and 100 µM H-D-Phe-pipecolyl-Arg-p-nitroanilide (S-2238, Biogenic, Montpellier, France). Before initiating factor Xa inhibition, the release of p-nitroaniline from 400 µM benzyl-CO-Ile-Glu-(gamma -OR)-Gly-Arg-p-nitroanilide (S-2222, Biogenic) by factor Xa was monitored for 10-15 min, until a steady state velocity of about 0.2 µM min-1 was reached. The inhibition reaction was initiated by the addition of a mixture of 0.2 µM 12A5 (or control IgG) and AT at various concentrations. The release of p-nitroaniline was monitored for up to 3 h using a Lambda 14 Perkin Elmer spectrophotometer, but only data corresponding to less than 10% substrate hydrolysis were used in the analysis. Estimates of the kon values were obtained by fitting data to the equation for slow-binding inhibition and corrected for the competition introduced by the substrate.

    RESULTS

12A5, a Monoclonal Antibody That Distinguishes AT-Protease Complexes from Native, Latent, and RCL-cleaved AT-- AT may adopt at least four conformations: native, heparin-activated, latent, and RCL-cleaved. A fifth conformation is likely to occur when AT is trapped in a complex with one of its targets (thrombin or factor Xa), but the precise structure of AT within this complex remains largely unknown. In an attempt to probe the elusive conformation of trapped AT, we prepared a panel of monoclonal antibodies; 12 were selected, because they recognized AT that had been coated on a microplate. To determine which conformer of AT was recognized, the monoclonal antibodies were coated onto a microplate, then AT (native, RCL-cleaved, or in complex with thrombin) was added, and sandwich ELISA developed with a polyclonal antibody directed against AT. Two monoclonal antibodies retained our attention, because they did not react with native or RCL-cleaved AT, while giving a strong signal with complexes; the other monoclonal antibodies reacted with all three forms of AT, indicating that selectivity of the former was not due to an experimental artifact. Thus, even though AT-protease complexes were not included in the immunization mixture, all the antibodies reacted with AT in complex. Although surprising at first, we reasoned that complexes might have formed with endogenous mouse proteases, and that a neoepitope inaccessible in the native protein might be exposed in partially denatured AT. To delineate more precisely the specificity of one of the monoclonal antibodies (12A5), we evaluated its affinities for native, latent, and heparin-activated AT, for AT with the RCL cleaved by thrombin or porcine pancreatic elastase, for AT in stable complex with thrombin or factor Xa, and for AT in complex with a peptide derived from the P14-P9 residues of its RCL. AT in complex with either thrombin or factor Xa bound to 12A5 in a dose-dependent fashion, as did the binary complex of AT with the P14-P9 peptide, although to a lesser extent, but binding of the other AT conformers remained undetected at concentrations as high as 1 µM (Fig. 2). In contrast, 12A5 recognized all forms of AT following polyacrylamide gel electrophoresis in the presence of SDS and immunoblotting, even after reduction of the disulfide bridges with beta -mercaptoethanol. Thus, a motif recognized by 12A5, inaccessible in native, heparin-activated, latent, and RCL-cleaved AT, was exposed in denatured and target-complexed AT. Taken together, these data suggested that steric hindrance, rather than a need for a specific conformation, limited the ability of 12A5 to interact with native or RCL-cleaved AT.


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Fig. 2.   Antibody 12A5 recognizes only complexes of AT with thrombin, factor Xa, or with the P14-P9 peptide derived from the RCL. Antibody 12A5 was coated onto microtiter wells, and AT in complex with thrombin (bullet ), AT in complex with factor Xa (open circle ), native AT (×), latent AT (black-square), heparin-activated AT (black-triangle), AT cleaved by porcine pancreatic elastase (), AT cleaved by thrombin (triangle ), or AT in complex with the P14-P9 peptide derived from the RCL (black-down-triangle ) was added and allowed to bind for 2 h. Sandwich ELISA was developed with a peroxidase-conjugated polyclonal IgG directed against AT.

12A5 Recognizes Pentapeptide DAFHK on the Penultimate Strand of beta -Sheet A-- As 12A5 recognized unfolded AT after reduction of the disulfide bonds, it was reasonable to expect that the neoepitope was a contiguous sequence of amino acids. We scanned two sets of biotinylated peptides, covering the entire sequence of AT. The first set simply divided AT sequence into 31 peptides that each were 14 amino acids in length, starting with His1 of AT; the second set divided AT sequence into 30 peptides (each also 14 amino acids in length) but starting with Cys8 of AT. Thus, each peptide overlapped 2 peptides of the alternate set by 7 amino acids (except peptides 1 and 31 of the first set). Peptides were individually linked to the well of a microtitration plate, and incubated with 12A5. ELISA was developed with a peroxidase-conjugated goat anti-mouse antibody. Only two peptides of the whole bank were able to bind 12A5: GRDDLYVSDAFHKA and SDAFHKAFLEVNEE, suggesting that the neoepitope included the shared motif SDAFHKA. Consistent with this hypothesis, 0.5 µM thrombin-AT displaced 50% of the binding of 10 nM 12A5 to peptide SDAFHKA coated on a microplate. Similarly, 0.2 µM peptide SDAFHK displaced 50% of the binding of 2 nM thrombin-AT to 12A5 coated on a microplate. To refine the neoepitope, we determined the shortest sequence that 12A5 could recognize by preparing a third set of peptides that consisted of successive, discrete, amino acid exclusions from either ends of peptide VSDAFHKAF. The shortest sequence recognized was DAFHK (Table I). The importance of each amino acid within the pentapeptide was evaluated by alanine scanning: aspartate, phenylalanine, and lysine were strictly required for 12A5 binding; in contrast, alanine in position 2 and histidine in position 4 could be substituted without affecting the binding capability of 12A5 (Table I). In the structure of AT, motif DAFHK is on the penultimate strand of beta -sheet A, and is not accessible to solvent (Fig. 3). The identity of the neoepitope was further confirmed by testing the reactivity of 12A5 with AT from various species. Motif DAFHK is conserved in human, bovine, sheep, porcine, and rabbit AT; all were recognized by 12A5 in immunoblotting analysis. In chicken AT, residue 366 is a glutamate instead of an aspartate. Consistent with the hypothesis that Asp366 was strictly required, 12A5 failed to recognize chicken AT (Fig. 4). Unexpectedly, mouse AT also contains the motif DAFHK and was recognized in immunoblotting, implying that 12A5 was an autoantibody. However, mammalian synthesis of an autoantibody directed against a cryptic region of a self-protein is not implausible; in fact, Nordling and Björk (33) have characterized a rabbit serum that recognizes the sequence VDLFSP shared by rabbit and human AT.

                              
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Table I
Identification of 12A5 epitope
Table shows binding of 12A5 to peptides derived from sequence Val364 to Phe372 of AT. Biotinylated peptides (linked to a microplate) were considered as able to grasp 12A5 (+) when signal was in excess of 8-fold that of the background. The minimum sequence recognized was DAFHK; within this sequence, only D, F, and K were found to be critical.


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Fig. 3.   Location of neoepitope DAFHK within the native and latent conformers of human AT. Stereo views of native (upper panel) and latent (lower panel) conformers of AT drawn using Molscript (version 3.1). Coordinates were obtained from the Protein Data Bank (access code 1ANT). Views are centered on beta -sheet A, and the location of the F-helix is indicated, as are the side chains of the residues critical for 12A5 binding (Asp366, Phe368, and Lys370). Consistent with the lack of recognition of both conformers by 12A5, Phe368 is partly masked to solvent by the loop connecting the F-helix to strand 3 of beta -sheet A.


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Fig. 4.   Antibody 12A5 is an autoantibody. Immunoblotting of sheep (lane 1), rabbit (lane 2), porcine (lane 3), and bovine plasma (lane 4); AT from all species share the motif DAFHK and were recognized by 12A5. In chicken AT (lane 5), residue 366 is a glutamate (instead of an aspartate); chicken plasma was not recognized by 12A5. Surprisingly, purified mouse AT (lane 6) was recognized by 12A5. Mouse AT contains a DAFHK sequence, implying that immunized mice synthesized an autoantibody against a cryptic region of their own protein. The last lane was obtained with purified human AT; the small lower band corresponds to beta -AT (45).

The Conformational Change Unveiled by 12A5 Is Subsequent to the Step Controlled by Ser195 of the Protease-- To achieve formation of a stable complex, serpin-protease interactions involve multiple steps, but in the path of AT inhibition, only the very last concerns the catalytic serine of the protease. S195A thrombin is an inactive variant, in which the catalytic serine has been replaced by alanine (8). S195A binds to AT with a kon value similar to that of thrombin, indicating that Ser195 of thrombin is not involved in the rate-limiting step of complex formation. The main difference between thrombin and S195A actually resides in the rate of complex dissociation; in contrast to the thrombin-AT complex, the S195A-AT complex is reversible (KI = 3 nM with heparin, 3 µM otherwise). Unless AT was denatured, the motif DAFHK appeared accessible to 12A5 only when AT was in complex with a peptide or a target. Thus, the combination of S195A and 12A5 constituted a remarkable tool to probe the concluding step of thrombin-AT complex formation; if the particular folding of AT unveiled by 12A5 takes place after formation of the initial complex, but before the irreversible step, AT bound to S195A must react with 12A5. On the contrary, if neoepitope exposure is concomitant with final stabilization of the complex, then motif DAFHK must remain invisible to 12A5, whether or not S195A adheres to AT. The high KI value of AT for S195A in the absence of heparin precluded study of 12A5 binding to the reversible complex, but saturation experiments were feasible in the presence of heparin. Hence, saturating amounts of S195A-AT complexes were not retained on a microplate coated with 12A5 (Fig. 5). This observation implied that neoepitope display was independent from initial binding of AT to thrombin; it was instead concomitant (or subsequent) to the catalytic step involving Ser195. Consistent with this hypothesis, 12A5 had no detectable influence on thrombin or factor Xa inhibition by AT; kon values were virtually identical, whether or not the target had been preincubated with saturating concentration of 12A5, and that reaction proceeded in the presence or not of heparin (Table II and Fig. 6). In fact, mice producing 12A5 were not abnormally ill, suggesting that autoantibody did not interfere with normal hemostasis, at least until neoepitope DAFHK was exposed (i.e. after formation of the stable complex). In this model, the autoantibody would not be harmful; the only consequence might have been a faster clearance of the protease-AT complexes.


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Fig. 5.   Antibody 12A5 fails to recognize the reversible complex of AT with S195A thrombin. Binding of the stable thrombin-AT complex (black-square) and of the reversible S195A-AT complex (black-triangle) to microtiter wells coated with 12A5. Sandwich ELISA was developed with a peroxidase-conjugated goat IgG directed against human AT. Lack of recognition does not originate from S195A-AT complex dissociation during the washing steps; middle curve (black-down-triangle ) represents binding of the S195A-AT complex to microtiter wells coated with a rabbit IgG directed against thrombin and revealed by a peroxidase-conjugated rabbit IgG directed against AT. Saturation suggests that washing does not dissociate the S195A-AT complex.

                              
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Table II
Association rate constants (kon) for thrombin and factor Xa inhibition by AT, with or without 12A5 antibody, and with or without heparin
Estimates of kon (in M-1 s-1) are given together with their standard error (in parentheses). With or without 12A5, kon values were similar, suggesting that 12A5 did not bind to AT until a stable complex was formed.


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Fig. 6.   Antibody 12A5 does not influence thrombin and factor Xa inhibition by AT. Progress curves of thrombin inhibition (upper panel) and factor Xa inhibition (lower panel) in the presence of heparin. Reactions were performed in the presence of 12A5 antibody (bullet ) or in the presence of a monoclonal antibody that does not recognize AT, thrombin, or factor Xa (open circle ). Solid lines were obtained by non-linear regression analysis of the data according to the equation for slow binding inhibition.


    DISCUSSION

Our data establish that AT in complex with thrombin or factor Xa differs from all conformers of known structure (native, heparin-activated, latent, or RCL-cleaved); they also imply that protease permits access to neoepitope DAFHK within the complex.

In none of the structures of AT available (18, 23, 25, 26) is the motif DAFHK entirely accessible to solvent. The side chains of Ala367 and His369 (dispensable for 12A5 binding) are internal; those of Asp366, Phe368, and Lys370 (critical for 12A5 binding) are directed toward the exterior, but in the native conformer they are partly hidden from solvent, except for Asp366 (Fig. 3). In latent AT, Asp366 contacts Val389 and Leu395 (P4 and P'2 residues of the RCL, respectively). Most remarkably, the side chain of Phe368 neighbors segment Ile198-Pro203 of the loop connecting the F-helix to strand 3 of beta -sheet A, both in the native and latent conformers of AT. Phe368 actually occupies a hydrophobic pocket lined by Val201, Ile202, Pro203, and Ala206; somehow, this pocket must open for 12A5 to bind. Lys370 also neighbors segment Ile198-Pro203; it is ion-paired to Asp200. Molecular contacts in the RCL-cleaved conformer of bovine AT are similar to those occurring in latent (human) AT. Thus, this neoepitope is constrained in native, as well as in latent and RCL-cleaved AT, and it is reasonable to predict that these constraints prevented 12A5 binding; conversely, formation of the stable complexes recognized by 12A5 must have released these constraints. The conformational change that accompanies complex formation and allows exposure of the neoepitope could be so dramatic, that the overall folding of AT has collapsed. This hypothesis would be consistent with our observation that 12A5 also recognized unfolded AT (i.e. blotted on nitrocellulose membrane or adsorbed on plastic wells). However, such a model would be difficult to reconcile with the dynamics of protease trapping by a serpin; in general, bonds are cleaved more efficiently in denatured substrates than in folded proteins (48). Thus, if formation of an initial Michaelis complex triggers gross disruption of AT folding, it would be expected that AT is a good substrate rather than an inhibitor. More satisfactory is a model in which the overall folding of AT is preserved: failure of 12A5 to react would originate from a steric hindrance that complex formation releases. Epitopes have been characterized that are recognized in the latent, RCL-cleaved, and complexed conformers, not in the native serpin; hence, RCL-cleaved and native conformers share a common overall folding, implying that limited conformational change indeed can trigger exposure of a neoepitope (27, 33, 49). Two limited alterations in the structure of AT would permit motif DAFHK to access solvent: partial disruption of beta -sheet A and/or motion of the loop connecting the F-helix to strand 3 of beta -sheet A (Fig. 3).

A number of studies suggest that several events, including formation of the stable complex, dramatically modify the structure of beta -sheet A in serpins. In type-1 plasminogen activator inhibitor, for instance, a temperature-dependent transition exposes the penultimate strand of beta -sheet A, as attested by its susceptibility to non-target proteases (50); at 0 °C, the native (inhibitory) conformer is cleaved within motif QALQK (i.e. within the region homologous to neoepitope DAFHK of AT); at 37 °C, the motif resists hydrolysis. The main structural rearrangement that seems to accompany complex formation consists of an insertion of the N-terminal (P) side of the RCL into beta -sheet A, but it is unclear which of the P14 to P1 residues are concerned (49, 51-54). It is likely that insertion is subsequent to the release by the protease of the C-terminal (P') side of the cleaved RCL (11, 55), whereas the P1 residue remains attached to the charge stabilizing system of the protease. Insertion of peptides with the same sequence as the RCL also causes conformational changes in AT; there, the extent of the insertion triggers various consequences, suggesting that many conformations are achievable (29, 42, 56, 57). Structural data obtained by x-ray diffraction (18, 25), fluorescence spectra data on the S380W variant of AT (17), immunochemical data (58), and susceptibility of the P1 arginine to chemical modification (59) all suggest that at least the P14 residue of the RCL is inserted in native (inhibitory) AT; this conformer was not recognized by 12A5 antibody. Insertion of a 7-amino acid peptide, homologous to residues P14-P8 of the RCL, induces a slow polymerization of AT (42), but insertion beyond the P8 residue, prevents polymerization (57). Neoepitope was recognized by 12A5 in the binary complex of AT with a peptide homologous to residues P14-P9 of its RCL. On the other hand, the neoepitope was no longer recognized by 12A5 in the conformer of AT having the RCL cleaved at the P5-P4 bond (i.e. with the RCL inserted up to the P5 residue), nor in AT cleaved at the P1-P'1 bond by thrombin (i.e. with the RCL inserted up to the P4 residue; Ref. 26). Finally, when a synthetic tetradecapeptide homologous to residues P14 to P1 of the RCL is locked in beta -sheet A, AT is a substrate rather than an inhibitor (30, 31). Thus, insertion of not less than two, but no more than nine, residues in beta -sheet A induced a particular conformation in AT that allowed 12A5 to bind the motif DAFHK. This neoepitope was also accessible to 12A5 in the stable complexes with thrombin or factor Xa. Therefore, it is tempting to conclude that AT in complex with either a target or the P14-P9 peptide share similar structural rearrangements: absent from the five- as well as from the six-stranded beta -sheet A conformers. Combined with the assumption that part of the RCL occupies beta -sheet A within the stable complex, the inference is that insertion concerns at least the P14-P13 residues of the RCL, at most the P14 to P6 segment.

An alternative to partial disruption of beta -sheet A would be that failure of 12A5 to react originates from a steric hindrance that is unrelated to the conformation of the penultimate strand of beta -sheet A. Unless it is masked, the motif DAFHK would be recognized by 12A5 in the five- as well as in the six-stranded conformers: structure of beta -sheet A would not be the limiting factor per se. Displacement of the loop connecting the F-helix to strand 3 of beta -sheet A would authorize neoepitope recognition by 12A5. Somehow, insertion of the RCL into beta -sheet A (as observed in latent and RCL-cleaved serpins) requires that the connection to the F-helix moves: otherwise, the RCL would have to sneak into a very narrow passage underneath the connecting loop. Fairly limited motion of this surface loop would suffice to clear access to motif DAFHK. Once the RCL is inserted, the surface loop could return to a location similar to that which it occupied initially, with the result that the neoepitope is masked again in the conformer with fully inserted RCL. It is unlikely that surface loop moves alone; comparison of RCL-cleaved and native serpins suggests that strands 1-3 of beta -sheet A, the F-helix, and the connecting loop constitute a mobile block that shifts relative to the remainder of the structure (60). Considering that the neoepitope was masked in both the native and RCL-cleaved conformers of AT, the inference is that the motif DAFHK is accessible to 12A5 only in the utmost distorted position of the mobile block, and that this distorted conformation is similar in the stable complex with a target. Consistent with this hypothesis, studies of a functional epitope in type-1 plasminogen activator inhibitor and of natural variants of AT or C1 inhibitor pointed out an unexpected role of the F-helix and of its connecting loop to the beta -sheet A (61-63).

The observation that motif DAFHK remained accessible within the complex AT-protease restricts the possible topologies of the complex (34-36). One of the proposals is that the enzyme ends in a location almost opposite to the RCL in intact serpins. This model is compatible with our data, as the protease would not cover motif DAFHK. However, it is difficult to reconcile the hypothesis that beta -sheet A is a six-stranded structure in the stable complex with our observation that neoepitope is not exposed following full insertion of the cleaved RCL. Thus, our data imply that if the protease rotates fully around the serpin, then either beta -sheet A is grossly unfolded within the complex (i.e. it is neither a five nor a six-stranded structure), or the F-helix has moved away to release motif DAFHK. Assuming on the contrary that the overall folding of AT is preserved, and that the cleaved RCL inserts partially into beta -sheet A, the complexed protease cannot rotate further than the F-helix around AT (i.e. about half-way), as it would otherwise cover the motif DAFHK. In this respect, it is interesting to note that 12A5 was incapable of even slowing down the inhibition reaction, and did not recognize the complex of AT with the S195A thrombin mutant; the lack of adverse effects on AT function suggests that only the very last step along the path of the reaction triggered exposure of the neoepitope. It is likely that this step is subsequent to the cleavage of the RCL.

    ACKNOWLEDGEMENTS

We thank Dr. Jean François Gaucher from the Université Paris V for Fig. 3, Dr. Paul Hopkins from the Gladstone Institute of Cardiovascular Disease San Francisco for helpful discussion, and Gregory Chang (Inserm U428) for careful reading of the manuscript.

    FOOTNOTES

* This work was supported by INSERM, France.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: INSERM U428, Faculté de Pharmacie, Université Paris V, 4 Av. de l'Observatoire, 75270 Paris Cedex 06, France Tel.: 33-1-53-73-98-28; Fax: 33-1-44-07-17-72; E-mail: lebonnie{at}infobiogen.fr.

    ABBREVIATIONS

The abbreviations used are: serpin, serine protease inhibitor; AT, antithrombin (previously called antithrombin III); RCL, reactive center loop (also called reactive site loop); BSA, bovine serum albumin; PBS, phosphate-buffered saline; ELISA, enzyme-linked immunosorbent assay.

    REFERENCES
Top
Abstract
Introduction
References
  1. Huber, R., and Carrell, R. W. (1989) Biochemistry 28, 8951-8966[Medline] [Order article via Infotrieve]
  2. Potempa, J., Korzus, E., and Travis, J. (1994) J. Biol. Chem. 269, 15957-15960[Free Full Text]
  3. Engh, R. A., Huber, R., Bode, W., and Schulze, A. J. (1995) Trends Biotechnol. 13, 503-510[CrossRef][Medline] [Order article via Infotrieve]
  4. Lawrence, D. A., Olson, S. T., Palaniappan, S., and Ginsburg, D. (1994) J. Biol. Chem. 269, 27657-27662[Abstract/Free Full Text]
  5. Schulze, A. J., Huber, R., Bode, W., and Engh, R. A. (1994) FEBS Lett. 344, 117-124[CrossRef][Medline] [Order article via Infotrieve]
  6. Olson, S. T., Bock, P. E., Kvassman, J., Shore, J. D., Lawrence, D. A., Ginsburg, D., and Björk, I. (1995) J. Biol. Chem. 270, 30007-30017[Abstract/Free Full Text]
  7. Fa, M., Karolin, J., Aleshkov, S., Strandberg, L., Johansson, L. B.-Å., and Ny, T. (1995) Biochemistry 34, 13833-13840[Medline] [Order article via Infotrieve]
  8. Stone, S. R., and Le Bonniec, B. F. (1997) J. Mol. Biol. 265, 344-362[CrossRef][Medline] [Order article via Infotrieve]
  9. Patston, P. A., Gettins, P., Beechem, J., and Schapira, M. (1991) Biochemistry 30, 8876-8882[Medline] [Order article via Infotrieve]
  10. Stone, S. R., and Hermans, J. M. (1995) Biochemistry 34, 5164-5172[Medline] [Order article via Infotrieve]
  11. Hopkins, P. C. R., Chang, W.-S. W., Wardell, M. R., and Stone, S. R. (1997) J. Biol. Chem. 272, 3905-3909[Abstract/Free Full Text]
  12. O'Malley, K. M., Nair, S. A., Rubin, H., and Cooperman, B. S. (1997) J. Biol. Chem. 272, 5354-5359[Abstract/Free Full Text]
  13. Loebermann, H., Tukuoka, R., Deisenhofer, J., and Huber, R. (1984) J. Mol. Biol. 177, 531-557[Medline] [Order article via Infotrieve]
  14. Baumann, U., Huber, R., Bode, W., Grosse, D., Lesjak, M., and Laurell, C. B. (1991) J. Mol. Biol. 218, 595-606[Medline] [Order article via Infotrieve]
  15. Baumann, U., Bode, W., and Huber, R. (1992) J. Mol. Biol. 226, 1207-1218[Medline] [Order article via Infotrieve]
  16. Mottonen, J., Strand, A., Symersky, J., Sweet, R. M., Danley, D. E., Geoghegan, K. F., Gerard, R. D., and Goldsmith, E. J. (1992) Nature 355, 270-273[Medline] [Order article via Infotrieve]
  17. Huntington, J. A., Olson, S. T., Fan, B., and Gettins, P. G. W. (1996) Biochemistry 35, 8495-8503[CrossRef][Medline] [Order article via Infotrieve]
  18. Jin, L., Abrahams, J. P., Skinner, R., Petitou, M., Pike, R. N., and Carrell, R. W. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 14683-14688[Abstract/Free Full Text]
  19. Song, H. K., Lee, K. N. L., Kwon, K.-S., Yu, M.-H., and Suh, S. W. (1995) FEBS Lett. 377, 150-154[CrossRef][Medline] [Order article via Infotrieve]
  20. Elliott, P. R., Lomas, D. A., Carrell, R. W., and Abrahams, J. P. (1996) Nat. Struct. Biol. 3, 676-681[Medline] [Order article via Infotrieve]
  21. Elliott, P. R., Abrahams, J.-P., and Lomas, D. A. (1998) J. Mol. Biol. 275, 419-425[CrossRef][Medline] [Order article via Infotrieve]
  22. Wei, A., Rubin, H., Cooperman, B. S., and Christianson, D. W. (1994) Nat. Struct. Biol. 1, 251-258[Medline] [Order article via Infotrieve]
  23. Schreuder, H. A., de Boer, B., Dijkema, R., Mulders, J., Theunissen, H. J. M., Grootenhuis, P. D. J., and Hol, W. G. (1994) Nat. Struct. Biol. 1, 48-54[Medline] [Order article via Infotrieve]
  24. Carrell, R. W., Stein, P. E., Fermi, G., and Wardell, M. R. (1994) Structure 2, 257-270[Abstract]
  25. Skinner, R., Abrahams, J.-P., Whisstock, J. C., Lesk, A. M., Carrell, R. W., and Wardell, M. R. (1997) J. Mol. Biol. 266, 601-609[CrossRef][Medline] [Order article via Infotrieve]
  26. Mourey, L., Samama, J. P., Delarue, M., Petitou, M., Choay, J., and Moras, D. (1993) J. Mol. Biol. 232, 223-241[CrossRef][Medline] [Order article via Infotrieve]
  27. Skriver, K., Wikoff, W. R., Patston, P. A., Tausk, F., Schapira, M., Kaplan, A. P., and Bock, S. C. (1991) J. Biol. Chem. 266, 9216-9221[Abstract/Free Full Text]
  28. Schulze, A. J., Baumann, U., Knof, S., Jaeger, E., Huber, R., and Laurell, C.-B. (1990) Eur. J. Biochem. 194, 51-56[Abstract]
  29. Schulze, A. J., Fronhert, P. W., Engh, R. A., and Huber, R. (1992) Biochemistry 31, 7560-7565[Medline] [Order article via Infotrieve]
  30. Björk, I., Nordling, K., Larsson, I., and Olson, S. T. (1992) J. Biol. Chem. 267, 19047-19050[Abstract/Free Full Text]
  31. Björk, I., Ylinenjärvi, K., Olson, S. T., and Bock, P. E. (1992) J. Biol. Chem. 267, 1976-1982[Abstract/Free Full Text]
  32. Debrock, S., and Declerck, P. J. (1995) FEBS Lett. 376, 243-246[CrossRef][Medline] [Order article via Infotrieve]
  33. Nordling, K., and Björk, I. (1996) Biochemistry 35, 10436-10440[CrossRef][Medline] [Order article via Infotrieve]
  34. Wright, H. T., and Scarsdale, J. N. (1995) Proteins: Struct. Funct. Genet. 22, 210-225[Medline] [Order article via Infotrieve]
  35. Stratikos, E., and Gettins, P. G. W. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 453-458[Abstract/Free Full Text]
  36. Wilczynska, M., Fa, M., Karolin, J., Ohlsson, P.-I., Johansson, L. B.-Å., and Ny, T. (1997) Nat. Struct. Biol. 4, 354-357[Medline] [Order article via Infotrieve]
  37. McKay, E. J. (1981) Thromb. Res. 21, 375-382[Medline] [Order article via Infotrieve]
  38. Gettins, P., and Harten, B. (1988) Biochemistry 27, 3634-3639[Medline] [Order article via Infotrieve]
  39. Urano, T., Strandberg, L., Johansson, L. B.-Å., and Ny, T. (1992) Eur. J. Biochem. 209, 985-992[Abstract]
  40. Munch, M., Heegaard, C. W., and Andreasen, P. A. (1993) Biochim. Biophys. Acta 1202, 29-37[Medline] [Order article via Infotrieve]
  41. Wardell, M. R., Chang, W.-S. W., Bruce, D., Skinner, R., Lesk, A. M., and Carrell, R. W. (1997) Biochemistry 36, 13133-13142[CrossRef][Medline] [Order article via Infotrieve]
  42. Chang, W.-S. W., Whisstock, J., Hopkins, P. C. R., Lesk, A. M., Carrell, R. W., and Wardell, M. R. (1997) Protein Sci. 6, 89-98[Abstract/Free Full Text]
  43. Harlow, E., and Lane, D. (1988) Antibodies: A Laboratory Manual, pp. 139-282, Cold Spring Harbor Laboratory, Plainview, NY
  44. Geysen, H. M., Rodda, S. J., Mason, T. J., Tribbick, G., and Schoofs, P. G. (1987) J. Immunol. Methods 102, 259-274[Medline] [Order article via Infotrieve]
  45. Picard, V., Ersdal-Badju, E., and Bock, S. C. (1995) Biochemistry 34, 8433-8440[Medline] [Order article via Infotrieve]
  46. Le Bonniec, B. F., Guinto, E. R., and Stone, S. R. (1995) Biochemistry 34, 12241-12248[Medline] [Order article via Infotrieve]
  47. Djie, M. Z., Le Bonniec, B. F., Hopkins, P. C. R., Hipler, K., and Stone, S. R. (1996) Biochemistry 35, 11461-11469[CrossRef][Medline] [Order article via Infotrieve]
  48. Le Bonniec, B. F., Myles, T., Johnson, T., Knight, C. G., Tapparelli, C., and Stone, S. R. (1996) Biochemistry 35, 7114-7122[CrossRef][Medline] [Order article via Infotrieve]
  49. Björk, I., Nordling, K., and Olson, S. T. (1993) Biochemistry 32, 6501-6505[Medline] [Order article via Infotrieve]
  50. Kjøller, L., Martensen, P. M., Sottrup-Jensen, L., Justesen, J., Rodenburg, K. W., and Andreasen, P. A. (1996) Eur. J. Biochem. 241, 38-46[Abstract]
  51. Shore, J. D., Day, D. E., Francis-Chmura, A. M., Verhamme, I., Kvassman, J., Lawrence, D. A., and Ginsburg, D. (1995) J. Biol. Chem. 270, 5395-5398[Abstract/Free Full Text]
  52. Wilczynska, M., Fa, M., Ohlsson, P.-I., and Ny, T. (1995) J. Biol. Chem. 270, 29652-29655[Abstract/Free Full Text]
  53. Eldering, E., Verpy, E., Roem, D., Meo, T., and Tosi, M. (1995) J. Biol. Chem. 270, 2579-2587[Abstract/Free Full Text]
  54. Lukacs, C. M., Zhong, J. Q., Plotnick, M. I., Rubin, H., Cooperman, B. S., and Christianson, D. W. (1996) Nat. Struct. Biol. 3, 888-893[Medline] [Order article via Infotrieve]
  55. Lawrence, D. A., Ginsburg, D., Day, D. E., Berkenpas, M. B., Verhamme, I. M., Kvassman, J.-O., and Shore, J. D. (1995) J. Biol. Chem. 270, 25309-25312[Abstract/Free Full Text]
  56. Mast, A. E., Enghild, J. J., and Salvesen, G. (1992) Biochemistry 31, 2720-2728[Medline] [Order article via Infotrieve]
  57. Fitton, H. L., Pike, R. N., Carrell, R. W., and Chang, W.-S. W. (1997) Biol. Chem. 378, 1059-1063[Medline] [Order article via Infotrieve]
  58. Asakura, S., Hirata, H., Okazaki, H., Hashimoto-Gotoh, T., and Matsuda, M. (1990) J. Biol. Chem. 265, 5135-5138[Abstract/Free Full Text]
  59. Pike, R. N., Potempa, J., Skinner, R., Fitton, H. L., McGraw, W. T., Travis, J., Owen, M., Jin, L., and Carrell, R. W. (1997) J. Biol. Chem. 272, 19652-19655[Abstract/Free Full Text]
  60. Stein, P., and Chothia, C. (1991) J. Mol. Biol. 221, 615-621[Medline] [Order article via Infotrieve]
  61. Debrock, S., and Declerck, P. J. (1998) Thromb. Haemost. 79, 597-601[Medline] [Order article via Infotrieve]
  62. Bruce, D., Perry, D. J., Borg, J.-Y., Carrell, R. W., and Wardell, M. R. (1994) J. Clin. Invest. 94, 2265-2274[Medline] [Order article via Infotrieve]
  63. Zahedi, R., Aulak, K. S., Eldering, E., and Davis, A. E., III (1996) J. Biol. Chem. 271, 24307-24312[Abstract/Free Full Text]


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