From the Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AY, United Kingdom
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ABSTRACT |
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We have isolated a full-length cDNA encoding an acetylcholinesterase secreted by the nematode parasite Nippostrongylus brasiliensis. The predicted protein is truncated in comparison with acetylcholinesterases from other organisms such that the carboxyl terminus aligns closely to the end of the catalytic domain of the vertebrate enzymes. The residues in the catalytic triad are conserved, as are the six cysteines which form the three intramolecular disulfide bonds. Three of the fourteen aromatic residues which line the active site gorge in the Torpedo enzyme are substituted by nonaromatic residues, corresponding to Tyr-70 (Thr), Trp-279 (Asn), and Phe-288 (Met).
High level expression was obtained via secretion from Pichia
pastoris. The purified enzyme behaved as a monomeric hydrophilic species. Although of invertebrate origin and possessing the above substitutions in the active site gorge residues, the enzyme efficiently hydrolyzed acetylthiocholine and showed minimal activity against butyrylthiocholine. It displayed excess substrate inhibition with acetylthiocholine at concentrations over 2.5 mM and was
highly sensitive to both active site and "peripheral" site
inhibitors. Northern blot analysis indicated a progressive increase in
mRNA for AChE B in parasites isolated from 6 days postinfection.
Acetylcholine (ACh)1 is
the major excitatory neurotransmitter which regulates motor functions
in the nematode Caenorhabditis elegans (1), and cholinergic
motor neurons have been identified in both free-living (1, 2) and
parasitic (3, 4) nematode species. In contrast to vertebrates (5) and
insects (6), C. elegans possesses multiple genes which
encode acetylcholinesterases (AChEs). Genetic studies originally
defined three separate genes, ace-1, ace-2, and
ace-3 (7-9) which encoded different catalytic forms of AChE
(10), although recent data resulting from cDNA cloning indicate
that C. elegans possesses at least four separate genes for
AChE (11).
In addition to possessing cholinergic motor neurons, many parasitic
nematodes secrete AChE into the culture medium when maintained in
vitro (12-14). A nonneuronal origin for these enzymes has been confirmed by enzyme- and immunohistochemistry, which demonstrated that
they are synthesized by and secreted from specialized amphidial and
secretory glands (13, 15-17). This remarkable phenomenon is exhibited
predominantly by nematodes which inhabit the gastrointestinal tract of
their vertebrate hosts and has provoked much discussion on the putative
physiological role of these enzymes (18, 19).
Analysis of the AChE secreted by the human hookworm Necator
americanus indicated that the enzyme existed as a nonamphiphilic dimer (G2na form) (20), whereas both
G1na and G2na forms
were identified in secreted products of Trichostrongylus colubriformis, a nematode parasite of sheep (21). We are utilizing Nippostrongylus brasiliensis, the adult stages of which
colonize the jejunum of rats, as a model system in which to study AChE secretion and have recently purified and characterized three variants of secreted AChE (22). These variants (designated A, B, and C) are all
G1na forms, with apparent masses of 74, 69, and
71 kDa, respectively, when resolved by SDS-polyacrylamide gel
electrophoresis. Substrate and inhibitor specificities defined them as
true AChEs rather than pseudocholinesterases. Although they show
broadly similar enzymatic properties, they can be distinguished by
subtle differences in molecular mass, inhibitor profiles, and excess
substrate inhibition and display distinct and characteristic mobilities
on nondenaturating PAGE (22).
These forms are differentially expressed by N. brasiliensis
during residence in the small intestine of the rat. Thus, parasites which have entered the duodenum from the stomach by three days postinfection exclusively secrete isoform A. Following moulting to the
adult stage and migration to a more distal position in the jejunum
however, a switch to expression of forms B and C occurs, with form B
progressively becoming the most abundant secreted enzyme (22-24). In
this report, we have derived amino-terminal amino acid sequence of form
B and utilized this to isolate a full-length cDNA clone. High level
expression of a recombinant form of the enzyme was obtained via
secretion from the methylotrophic yeast Pichia pastoris. The
unusual primary structure of the enzyme is discussed in relation to the
enzymatic properties of the recombinant enzyme and to the structure and
properties of AChEs from other organisms.
Parasites--
N. brasiliensis were isolated from the
small intestine of male Sprague-Dawley rats between days 3 and 10 postinfection as described previously (24). Excretory/Secretory (ES)
products were collected from culture supernatants as described (22,
24), concentrated in Centricon 10 microconcentrators (Amicon 4205), washed into 50 mM phosphate-buffered saline, pH 7.4, and
the protein concentration determined via the Bradford assay.
Cloning and Sequencing--
We utilized a 5' primer tailored to
the 6 amino-terminal amino acids of AChE B (DDGPTV) and containing a
NotI restriction site
(5'-atgcggccgcGA(T/C)GA(T/C)GGNCCNACNGT-3'), and a 3' primer based on
the conserved sequence flanking the active site serine residue (FGESAG)
linked to a XhoI restriction site
(5'-atctcgagCCNGCN(C/G)(A/T)(C/T)TC(T/G/A)CC(A/G)AA-3') to amplify a
fragment by RT-PCR which would putatively encode the amino-terminal
region of AChE B (lowercase indicates nucleotides added for cloning
purposes). A standard 100-µl PCR reaction was utilized, using 30 ng
of cDNA, 500 ng of each primer, and 30 cycles of: 94 °C for
30 s, 54 °C for 1 min, 72 °C for 2 min. This procedure yielded a product of 580 bp, which was subcloned into pBluescript II
(Stratagene) and sequenced to confirm that it encoded a cDNA fragment which was homologous to AChEs. We then utilized this fragment
to screen a cDNA library constructed in Expression in P. pastoris--
N.
brasiliensis-secreted AChE B was expressed in P. pastoris via the expression plasmid pPICZ Purification of Native and Recombinant AChE--
The native
enzymes were purified from ES products of worms isolated 8 days
postinfection by affinity chromatography on
1-methyl-9-[N Denaturing and Nondenaturing Electrophoresis--
SDS-PAGE was
performed on 12% polyacrylamide gels followed by staining with
Coomassie Brilliant Blue. Purified recombinant AChE and ES products
were resolved under nondenaturing conditions by electrophoresis in
7.5% polyacrylamide gels in Tris-borate-EDTA (TBE) buffer, pH 8.0, and
enzyme activity was assayed by the method of Karnovsky and Roots
(25).
Sucrose Density Centrifugation--
Sedimentation analysis was
performed in 2-20% sucrose gradients (11 ml, with a 0.5-ml cushion of
50% sucrose) in phosphate-buffered saline in the presence or absence
of 1.0% Triton X-100. The purified enzyme was applied to the gradient
and centrifuged for 16 h at 36,000 rpm at 4 °C in an SW41 rotor
(170,000 × g). Fractions (0.25 ml) were collected and
assayed for AChE activity. Bovine liver catalase (11.3 S) and
Escherichia coli alkaline phosphatase (6.1 S) were included
as internal standards.
Northern Blotting--
Total RNA was isolated from parasites as
described previously (26). For Northern blots, RNA was denatured in
glyoxal, 10 µg per track separated on 1.2% agarose gels and
transferred to nylon filters. Equal loading of RNA was confirmed by
ethidium bromide staining of gels prior to blotting and methylene blue staining of filters following transfer. Filters were hybridized with
the full-length C43 cDNA probe labeled by random priming in 5×
SSC, 0.25 mg ml AChE Activity and Substrate and Inhibitor
Specificities--
AChE activity was determined by the method of
Ellman (57) with 1 mM acetylthiocholine (ASCh) iodide as
substrate in the presence of 1 mM
5,5'-dithiobis(2-nitrobenzoic acid) in 100 mM sodium
phosphate, pH 7.0, at 20 °C. The reaction was monitored by measuring
the absorbance at 412 nm, and hydrolysis of ASCh was calculated from
the extinction coefficient of 5,5'-dithiobis(2-nitrobenzoic acid) (27).
One unit of AChE is defined as 1 µmol of substrate hydrolyzed per min
at 20 °C. The Km value for ASCh was determined by
linear regression from plots of 1/V against 1/S, utilizing substrate at a range of concentrations between 25 and 200 µM (i.e. below substrate inhibition).
Inhibition constants (Ki) for eserine, BW284C51,
propidium iodide, and gallamine were determined using two fixed
inhibitor and six ASCh concentrations (0.05-0.20 mM).
Assays were carried out in triplicate in the presence or absence of
inhibitor. Kinetic constants were determined using Graphpad Prism 2.0 (San Diego, CA).
cDNA Cloning of Secreted AChE B--
We purified native AChEs
(forms B and C) from ES products of worms isolated 8 days postinfection
by affinity chromatography on
1-methyl-9-[N Structural Features of N. brasiliensis Secreted AChE B--
The
most pertinent features of AChE B defined by the primary structure are
illustrated in Fig. 2 in which it is
aligned with that of C. elegans ACE-1 (28) and the T subunit
of T. californica AChE (29). The residues in the catalytic
triad, Ser-193, Glu-342, and His-466 (Ser-200, Glu-327, and His-440 in
Torpedo), are conserved. Six cysteine residues are present
(62-89, 247-276, and 428-543) that define the position of the three
intramolecular disulfide bonds conserved in cholinesterases (67-94,
254-265, and 402-521; Refs. 30, 31), and an additional two cysteine
residues are present in positions 232 and 263 in the N. brasiliensis sequence. The protein is truncated at the carboxyl
terminus by 45 residues relative to C. elegans ACE-1 or by
37 residues relative to the T subunit of the Torpedo enzyme.
There are three consensus sequences for N-linked
glycosylation, at positions 124, 377, and 480.
Eleven of the fourteen aromatic residues which line the wall of the
active site gorge in the Torpedo enzyme (32) are either conserved or show conservative substitutions in the nematode sequence, including Trp-79 (Trp-84) which binds the quaternary ammonium group of
acetylcholine in the active site (33, 34). The three residues which
show nonconservative substitutions are Thr-65 (Tyr-70), Asn-290
(Trp-279), and Met-301 (Phe-288).
Expression of AChE B in N. brasiliensis--
Expression of AChE B
during residence of N. brasiliensis in the intestinal tract
of the mammalian host was determined by Northern blotting, utilizing
RNA from parasites recovered at different time points postinfection and
the C43 cDNA as a probe. Fig. 3 demonstrates that the levels of mRNA for the secreted AChE remained relatively constant in parasites isolated between days 3 and 6 postinfection but, thereafter, rose progressively when assayed 8 and 10 days postinfection.
Expression in P. pastoris--
We next expressed N. brasiliensis AChE B in the methylotrophic yeast P. pastoris, utilizing the yeast
Recombinant AChE B was resolved by sucrose density gradient
centrifugation in a single peak at 4.5 S. There was no shift in sedimentation in the presence of 1% Triton X-100, indicative of a
monomeric nonamphiphilic (G1na) enzyme (data
not shown). The purified enzyme was next subjected to nondenaturing
PAGE and stained for AChE activity, and Fig. 5 demonstrates that it resolved as a
fast-migrating single band that co-migrated with form B of the native
secreted parasite AChEs.
Substrate and Inhibitor Specificities--
The specificity of AChE
B for a range of substrates was assayed, and the results are shown in
Fig. 6, demonstrating that ASCh was the
preferred substrate. Thus, at a substrate concentration of 1 mM, the rate of hydrolysis of A[
We next assessed the susceptibility of the recombinant enzyme to
cholinesterase inhibitors. Activity was unaffected by the butyrylcholinesterase inhibitor iso-OMPA at concentrations up to 10 mM (data not shown), but it was highly sensitive to eserine and BW284c51, the latter a specific inhibitor of AChEs (35). Both
eserine and BW284c51 inhibited in a competitive manner, and the
Ki values were calculated at 3.6 ± 1.2 and
6.4 ± 1.9 nM (n = 4), respectively.
The "peripheral" site ligands, propidium iodide and gallamine, also
inhibited AChE activity but in a noncompetitive manner, with
Ki values of 0.22 ± 0.03 and 0.80 ± 0.07 µM (n = 3), respectively (Fig.
7).
The phenomenon of cholinesterase secretion by parasitic nematodes
was first reported almost 30 years ago (12, 13, 15) and yet still
presents a conundrum which awaits a satisfactory explanation. We are
attempting to address the physiological significance of this process
and present here a molecular characterization of one of the enzymes
secreted by N. brasiliensis. As would be expected for a
secreted protein, it is hydrophilic, and the properties of the
recombinant AChE B are essentially the same as those of the native
enzyme (22). The specific activity of the native enzymes purified by
acridinium affinity chromatography (685 units mg Derivation of the primary structure of the protein from the cDNA
sequence provides an explanation for its monomeric nature, as the
carboxyl-terminal cysteine implicated in dimer formation is missing. In
fact, the carboxyl terminus is severely truncated in comparison with
vertebrate AChEs and therefore bears no resemblance to the peptides
that define hydrophobic (H) or tailed (T) subunits (36). Fig. 2
illustrates that the carboxyl terminus of the parasite enzyme aligns at
a position approximating to the end of the catalytic domain of
Torpedo AChE. In this respect, the parasite enzyme is similar to AChE from Bungarus fasciatus venom, which is also
unusual in that it is a hydrophilic monomeric enzyme with a truncated carboxyl terminus, although the carboxyl-terminus of ACE B is 12 residues shorter than that of the Bungarus AChE and thus
lacks the basic residues associated with the latter enzyme which are cleaved on secretion (37, 38).
In many other respects, the sequence of the N. brasiliensis
AChE B shows features consistent with AChEs from diverse species. The
residues which constitute the catalytic triad are conserved, as are the
six cysteine residues implicated in disulfide bond formation. An
additional two cysteine residues are present in the N. brasiliensis sequence, however, at positions 232 and 263. The
latter lies in an insertion of 17 amino acids (relative to Torpedo), which by analogy with the structure of the
T. californica AChE (32) most probably forms part of a loop
of 30 residues at the molecular surface stabilized by the second
disulfide bond.
One of the most striking features of the structure of the
Torpedo enzyme is the active site gorge, about 20-Å long,
which penetrates halfway into the enzyme (32). Fourteen aromatic
residues line a substantial part of the surface of the gorge. These
residues and flanking sequences are highly conserved in AChEs from
different species and are thought to delineate a substrate guidance
mechanism. Eleven of these residues are either conserved or show
conservative substitutions in the N. brasiliensis AChE. Two
of the aromatic residues (Tyr-70 and Phe-288 in Torpedo)
that are substituted in the nematode sequence by Thr-65 and Met-301 are
also substituted by nonaromatic residues in mammalian
butyrylcholinesterases (39) and C. elegans ACE-1 (28).
Mutagenesis studies on Torpedo and human AChE have shown
that Phe-288 and Phe-290 dictate substrate specificity, most probably
via steric occlusion but also possibly by stabilizing the substrate in
an optimal position for catalysis (34, 40, 41). It has been suggested
that the intermediate substrate specificity of certain invertebrate
enzymes such as C. elegans ACE-1 and Drosophila
melanogaster AChE (both enzymes hydrolyze BuSCh at approximately
50% the rate of ASCh) can be explained by the substitution of Phe-288
by glycine and leucine, respectively (28, 42). Replacement of Phe-288
in Torpedo and human AChE by nonaromatic residues greatly
enhanced the ability of these enzymes to hydrolyze BuSCh, in addition
to conferring sensitivity to inhibition by iso-OMPA (34, 40, 41). It is therefore surprising that Phe-288 is replaced by a methionine residue
in the N. brasiliensis sequence, as the enzyme shows little activity against BuSCh and no inhibition by iso-OMPA even at very high
concentrations (10 mM). We assume that alternative residues must restrict substrate and inhibitor accessibility. Phe-290 and Phe-331 in Torpedo AChE are both substituted by Trp in the
N. brasiliensis enzyme (Fig. 2), and it is possible that the
bulkier side chain restricts the size of the acyl pocket.
There are 3 consensus sequences for N-linked glycosylation,
at positions 124, 377, and 480 in the N. brasiliensis
sequence. Although it is not yet clear which of these sites are
glycosylated in the native protein, only one of these (Asn-480) is
closely aligned to a glycosylation site (Asn-557) in the
Torpedo enzyme (43). Asn-124 is unlikely to be glycosylated
as it is positioned directly after Tyr-123 (Tyr-130 in
Torpedo), one of the aromatic residues lining the active
site gorge.
N. brasiliensis AChE B is highly sensitive to inhibition by
eserine and BW284c51, with Ki values of 3.6 and 6.4 nM, but is also sensitive to the peripheral site ligands
propidium iodide and gallamine with Ki values of
0.22 and 0.80 µM, respectively (Fig. 7). The
Ki for BW284c51 is similar to that of
Torpedo AChE at 2 nM, whereas those for the
latter inhibitors are approximately an order of magnitude lower:
propidium and gallamine inhibited Torpedo AChE with
Ki values of 2.8 and 15 µM,
respectively (44). Peripheral site ligands interact with diverse
subsets of residues, and recently the low sensitivity of the B. fasciatus AChE to these inhibitors was attributed to substitution
of the aromatic residue Tyr-70 located at the entrance of the active
site gorge by methionine and of an acidic residue (Asp/Glu) at position
285 by lysine (38). Tyr-70 is replaced by threonine in N. brasiliensis AChE and serine in C. elegans ACE-1,
although both enzymes remain sensitive to peripheral site ligands:
propidium inhibits ACE-1 with a Ki of 1.3 µM (28). Although our alignment and that of Arpagaus
et al. (28) place an alanine and arginine residue at
position 285 in the two nematode AChEs, both these residues are
followed immediately by glutamate. Two other residues that have been
implicated in inhibition by peripheral site ligands are Trp-279 and
Trp-84 (Trp-286 and -86 in human AChE). Trp-279 lies at the rim of the
active site gorge, and the mutant W279A shows strongly reduced
inhibition by propidium (40, 34). Trp-84 is located in the active site (32) and binds the quaternary ammonium of choline (33, 34), and
substitution with alanine renders the enzyme almost completely refractory to inhibition by propidium (34). A model has been proposed
linking these two observations in which binding of propidium to Trp-279
induces a conformational change relayed via residues along the gorge,
resulting in reorientation of Trp-84 which interferes with
stabilization of substrate enzyme complexes (34). Although Trp-84
(Trp-79) is conserved in N. brasiliensis AChE B, Trp-279 is
replaced by Asn-290 (Fig. 2), highlighting a further discrepancy in the
basis for inhibition by peripheral site ligands in this enzyme.
These anomalies could be clarified by structural analysis of the
N. brasiliensis AChE. It is possible to obtain only
microgram quantities of native enzyme, but the recombinant enzyme is
fortuitously expressed in P. pastoris at very high levels.
Continuous culture for 7 days resulted in approximately 35 mg of enzyme
per liter of culture medium. Expression of rat and Bungarus
AChE in P. pastoris resulted in maximal levels of
approximately 1 and 2 mg per liter, respectively, when cultured for the
same period of time, although it was necessary to generate constructs
truncated at the carboxyl terminus to exclusively produce
nonamphiphilic monomers that were secreted more efficiently (45). It is
unclear why the N. brasiliensis secretory AChE should be
particularly amenable to high level expression in this system, although
these are highly hydrophilic proteins that are released naturally in
monomeric form (22). Moreover, they show an extremely high rate of
turnover, such that adult worms have been observed to synthesize and
secrete 4-fold the total body content of AChE when maintained in
culture for 24 h (46). Northern blot analysis (Fig. 3) suggests
that the progressive increase in parasite AChE levels during residence
in the jejunum (47) is regulated primarily at the level of
transcription. Moreover, the timing is consistent with a response to
elements of the host immune system, as previously suggested (48,
49).
Given these observations, it is interesting to speculate on the
putative role of acetylcholinesterases secreted not just by a cell but
by a whole organism, as the target for these enzymes must lie in the
intestinal tract of the mammalian host. One possibility lies in the
suggestion that AChEs might play a role in regulating differentiation
of hematopoietic cells by promoting apoptosis (50). An alternative
possibility lies in the role of the enteric nervous system in
regulating secretory processes of enterocytes. Nerve fibers in the
mucosa terminate subjacent to the basement membrane of epithelial and
enteroendocrine cells, on which muscarinic acetylcholine receptors
(mAChRs) have been localized (51). Muscarinic AChR agonists evoke
chloride secretion from intestinal epithelial cells, and the
electrogenic flux creates an osmotic gradient resulting in passive
movement of water in the lumen (52). In addition, these agonists evoke
mucous secretion from goblet cells (53) and exocytosis of Paneth cells,
epithelial granulocytes located at the base of the crypts of
Lieberkühn (54). The granules of these cells contain a variety of
antimicrobial products, including an array of pore-forming proteins
termed cryptdins or crypt defensins (55), although to our knowledge
these have not been tested for toxicity against macroparasites such as nematodes.
These secretory events constitute components of innate immunity which
most likely contribute to expulsion of pathogens and noxious agents
from the gastrointestinal tract and are regulated in part by the
enteric nervous system. It is therefore an attractive proposition that
AChEs secreted by nematode parasites act to inhibit secretory responses
by hydrolyzing acetylcholine. The reagents generated by cloning and
high-level expression of nematode secretory AChEs will help to define a
biological role for these enzymes, in addition to clarifying the
molecular basis for their enzymatic properties.
INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
ZAP (Stratagene) and
isolated several clones, which were sequenced on both strands by
dideoxy chain termination following subcloning into pBluescript.
(Invitrogen). The
nucleotide sequence encoding the mature enzyme was amplified from
cDNA clone C43 using the sense primer
5'-tatactgcagATGATGGTCCGACGGTGGTG-3' and the antisense primer
5'-attatctagaTTGCCTTTAGCTTCATCAAT-3'. The fragment was cloned into
pPICZ
B cut with PstI and Xbal, and the reading
frame was verified by partial sequencing of the recombinant plasmid,
which was then linearized with SacI. The construct was integrated into competent P. pastoris X-33 via homologous
recombination according to the instructions of the manufacturer
(Invitrogen), and transformants were confirmed by PCR with
oligonucleotide primers homologous to pPICZ
B sequences flanking the
insertion site. The construct thus encoded the mature AChE B protein
with an amino-terminal signal peptide provided by the pre-pro sequence
of the
-mating factor of Saccharomyces cerevisiae and
with a carboxyl-terminal polyhistidine tag. Recombinant yeast were
grown in BMGY medium (1% yeast extract, 2% peptone, 0.1 M
potassium phosphate, pH 6.0, 1.34% yeast nitrogen base, 400 ng
ml
1 biotin, 1% glycerol) to saturation (2 days), and
expression was induced in BMMY medium (same as BMGY, but with glycerol
replaced by 1% methanol) at 30 °C. Culture supernatants were
harvested at different times postmethanol induction and analyzed for
AChE activity.
-(
-aminocaproyl)-
-aminopropylamino]-acridinium bromide hydrobromide-Sepharose 4B (a kind gift of Prof. I. Silman) as
described previously (22). Amino-terminal amino acid sequence of the
major AChE isoform (form B) was obtained following resolution by 10%
SDS-PAGE and blotting onto a polyvinylidene difluoride membrane
(Bio-Rad) and was performed by Severn Biotech (Kidderminster, UK). The
recombinant AChE was purified from culture supernatants of transformed
P. pastoris by nickel-charged chelating Sepharose chromatography. Chelating Sepharose (Amersham Pharmacia Biotech) was
charged with 1% NiSO4, washed with distilled water and
equilibrated with 50 mM sodium phosphate, pH 8.0, containing 300 mM sodium chloride (buffer A). The yeast
culture supernatant was clarified by centrifugation at 25,000 × g for 10 min at 4 °C, concentrated on an Amicon cell, and
dialyzed against buffer A. The dialysate was loaded onto the column at
a flow rate of 40 ml h
1, which was subsequently
extensively washed with buffer A and then buffer A plus 10 mM imidazole. The enzyme was then eluted from the column
with buffer A plus 200 mM imidazole, and the purity of the
enzyme was determined by SDS-PAGE.
1 salmon sperm DNA, 0.25% SDS, 0.25%
dried milk powder at 65 °C with 106 cpm
ml
1 of probe, and blots were washed in 0.1× SSC, 0.25%
SDS at 65 °C.
RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
-(e-aminocaproyl)-
-aminopropylamino]-acridinium bromide hydrobromide-Sepharose 4B, resolved the enzymes by
SDS-PAGE, and blotted them onto a polyvinylidene difluoride membrane.
This procedure resulted in the determination of just six amino acids of
amino-terminal sequence of the major isoform (form B) as DDGPTV. This
information allowed us to design a primer that was utilized in
combination with another to the conserved sequence flanking the active
site serine residue of AChEs (FGESAG) to amplify a cDNA fragment of
approximately 580 bp by RT-PCR, using RNA from parasites isolated 5 days postinfection. This cDNA fragment was sequenced and showed
significant homology to cholinesterases from diverse sources and was,
therefore, used to screen a cDNA library constructed in
ZAP.
Several clones were isolated by this procedure and sequenced. The
sequence of the longest clone (C43, 1799 bp) is shown is Fig.
1. It contains a short (12 bp)
5'-untranslated region, a single open reading frame of 1680 nucleotides, and a 3'-untranslated region of 107 bp, containing a
consensus polyadenylation site (AATAAA, underlined) and a poly(A) tail.
Analysis of the amino acid sequence encoded by C43 predicts a signal
peptide of 19 residues, defined by the amino-terminal sequence of the
native protein (underlined). The primary structure of the mature
protein exhibits clear similarities to cholinesterases from numerous
species, including 30% identity to ACE-1 of C. elegans (28)
and 35% identity to Torpedo californica AChE (29).
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Fig. 1.
Nucleotide and derived amino acid sequence of
cDNA encoding the secreted AChE B of N. brasiliensis. The amino acid sequence is numbered from
the initiating methionine, and the amino-terminal sequence of the
mature secreted protein is underlined. Residues predicted to
comprise the catalytic triad of the enzyme are circled, and
three potential sites for N-linked glycosylation are
boxed. A consensus sequence for polyadenylation (AATAAA) in
the 3'-untranslated sequence is also underlined.
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Fig. 2.
Alignment of N. brasiliensis, T. californica,
and C. elegans AChE sequences.
The derived amino acid sequence of the N. brasiliensis
secreted AChE (Nb) was aligned with that of the T subunit of
T. californica (Tc) and ACE-1 from C. elegans (Ce) using the CLUSTALW program (56). The
N. brasiliensis and the T. californica AChEs are
numbered from amino terminus of the mature proteins (marked
"1"). The residues in the catalytic triad (Ser-His-Glu)
are depicted with an asterisk, and the position of three
potential sites for N-linked glycosylation marked with a
square. Cysteine residues conserved at positions which are
involved in intramolecular disulfide bonds are indicated by solid
triangles, whereas open triangles depict the two
additional cysteine residues in the N. brasiliensis AChE.
The positions of aromatic residues that line the active site gorge in
Torpedo AChE are marked with circles. Solid
circles indicate conserved residues or conservative substitutions
at these positions in the N. brasiliensis AChE, whereas
open circles indicate nonaromatic residues. The protein is
truncated at the carboxyl-terminus. For orientation, the
arrow indicates the branch point for T and H subunits in
Torpedo.
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Fig. 3.
Expression of AChE B is up-regulated during
residence in the intestinal tract. Total RNA from parasites
recovered from the jejunum of rats at different time points from day 3 (d3) to day 10 (d10) postinfection were subjected
to analysis by Northern blotting, utilizing the C43 cDNA as a
hybridization probe. A single mRNA species of 2.3 kilobases is
indicated by an arrow. The levels of this mRNA are
progressively elevated from day 8 postinfection.
-mating factor signal peptide to
direct secretion of the enzyme, and colonies were selected that
expressed high levels of AChE. In a representative example, AChE
activity was detected in the culture medium assayed just 30 min after
methanol induction. The concentration of total protein secreted by this
recombinant yeast clone rose from approximately 6 µg
ml
1 at 24 h to 27 µg ml
1 at 5 days
postinduction (Fig. 4A),
although in practice we changed the culture medium daily. Fig.
4B, lane 2 shows that AChE B constituted approximately 90% of the total secreted protein. After 2 days postinduction, the medium was collected and the recombinant enzyme purified by nickel-chelating chromatography. The purified enzyme had a
high specific activity when assayed against ASCh of 2,080 units
mg
1. It displayed an apparent mass of 69 kDa when
resolved by SDS-PAGE under nonreducing conditions and one of 72 kDa
under reducing conditions (Fig. 4B, lane 3),
indicative of a monomeric protein with intramolecular disulfide
bonds.
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Fig. 4.
Expression of N. brasiliensis
AChE B in P. pastoris. A,
kinetics of secretion into the culture medium following induction of
expression with methanol. Total secreted protein is indicated by
closed circles, and AChE activity is indicated by open
circles. B, purification of recombinant AChE. Culture
medium from P. pastoris X-33 wild-type cells (lane
1) or cells transfected with cDNA encoding N. brasiliensis AChE (lane 2), and the purified enzyme
(lane 3) were resolved via 12% SDS-PAGE under reducing
conditions. The gel was fixed and stained with Coomassie Blue. The
position of molecular mass markers is shown in kDa.
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Fig. 5.
Resolution of AChE B by nondenaturing
PAGE. Secreted proteins from the culture medium of P. pastoris X-33 wild type cells (lane 1) and purified
recombinant AChE (lane 3) were resolved by nondenaturing
7.5% PAGE alongside native secreted AChEs from parasites collected 4 days postinfection (lane 2). AChE activity was visualized
according to Karnovsky and Roots (25). The migration of native
secretory enzymes designated as forms A, B, and C (22) is
indicated.
M]SCh, PSCh, and BuSCh
relative to ASCh were 61, 15, and 5%, respectively. Excess substrate
inhibition was observed for the former substrates at concentrations
exceeding 2.5 mM, and the Km value for
the enzyme was calculated at 0.23 ± 0.10 mM
(n = 8).
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Fig. 6.
Substrate specificity of AChE B. The
rate of hydrolysis of acetylthiocholine ( ),
acetyl-[
-methyl]thiocholine (
), propionylthiocholine (
) and
butyrylthiocholine (
) were determined as described under
"Experimental Procedures," utilizing a range of substrate
concentrations between 0.05 and 10 mM.
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Fig. 7.
Sensitivity of AChE B to inhibitors.
Inhibition of AChE activity by eserine, BW284c51, and the peripheral
site ligands propidium iodide and gallamine was assessed. Assays were
performed with varying concentrations of ASCh in the absence ( ) and
the presence of 1 nM (
) or 5 nM (
)
eserine and BW284c51, and 0.1 µM (
) or 0.5 µM (
) propidium iodide and gallamine. Lineweaver-Burk
plots (1/V versus 1/S) are shown,
indicating competitive inhibition by eserine (Ki = 3.6 ± 1.2 nM) and BW284c51 (Ki = 6.4 ± 1.9 nM) and noncompetitive inhibition by
propidium (Ki = 0.22 ± 0.03 µM)
and gallamine (Ki = 0.80 ± 0.07 µM).
DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
1) was
considerably lower than that of the recombinant enzyme purified by
nickel-chelating chromatography (2,080 units mg
1)
however, and this is consistent with previous data (22). We attribute
this to residual binding of decamethonium to the native enzymes, which
can only be purified in low microgram quantities. Partial purification
of native AChE B without the use of an affinity ligand gave a value of
1,450 units
mg
1,2
suggesting that the estimation of specific activity for the recombinant enzyme is close to that of the native enzyme.
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ACKNOWLEDGEMENTS |
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We gratefully acknowledge Drs. Kurt Giles, Xavier Cousin, and Leo Pezzementi for valuable discussion.
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FOOTNOTES |
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* This work was supported by the Wellcome Trust.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF052508.
Present address: Dept. of Histochemistry, Imperial College of
Science, Technology and Medicine, Hammersmith Campus, Ducane Rd.,
London W12 0NN, UK.
§ Present address: Dept. of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322.
¶ Supported by Minority International Research Training Grant T37-TW00046 from the Fogarty International Center at the National Institutes of Health. Present address: Dept. of Microbiology and Immunology, University of North Carolina at Chapel Hill, NC 27599-7290.
To whom correspondence should be addressed. Fax: +44 171 225 0960; E-mail: m.selkirk{at}ic.ac.uk.
2 Grigg, M. E., unpublished observations.
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ABBREVIATIONS |
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The abbreviations used are: ACh, acetylcholine; AChe, acetylcholinesterase; PAGE, polyacrylamide gel electrophoresis; ES, excretory/secretory; RT-PCR, reverse transcriptase polymerase chain reaction; bp, base pair(s); ASCh, acetylthiocholine; BuSCh, butyrylthiocholine; iso-OMPA, tetraisopropyl pyrophosphoramide; BW284c51, 1,5-bis(4-allyldimethylammoniumphenylpentan-3-one dibromide.
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REFERENCES |
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