Multiple p44 Genes Encoding Major Outer Membrane Proteins Are Expressed in the Human Granulocytic Ehrlichiosis Agent*

Ning Zhi, Norio Ohashi, and Yasuko RikihisaDagger

From the Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210-1093

    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Human granulocytic ehrlichiosis (HGE) is caused by infection with an obligatory intracellular bacterium, the HGE agent. We previously cloned a gene encoding HGE agent 44-kDa major outer membrane protein and designated it p44. In this study, we (i) identified five different mRNAs that are transcribed from p44-homologous genes in the HGE agent cultivated in HL-60 cells; (ii) cloned genes corresponding to the mRNAs from the genomic DNA of the HGE agent; (iii) showed that the genes being expressed were not clustered in the HGE agent genome; (iv) estimated that a minimum copy number of the p44-homologous genes in the genome is 18; (v) detected two different P44-homologous proteins expressed by the HGE agent; and (vi) demonstrated existence of antibodies specific to the two proteins in sera from patients with HGE. These findings showed that p44 multigenes have several active expression sites and the expression is regulated at transcriptional level, suggesting a potentially unique mechanism for generating the diversity in major antigenic outer membrane proteins of the HGE agent. Characterization of p44-homologous genes expressed by the HGE agent in a tissue culture would assist in understanding a role of the p44 multigene family in pathogenesis and immune response in HGE.

    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Human granulocytic ehrlichiosis (HGE),1 a tick-borne zoonosis, was first reported in 1994 and increasingly recognized in the United States (1-3). Serologic and PCR analyses suggest that HGE also exists in Europe (4-6). HGE is characterized by chills, headache, myalgia, and hematological abnormalities, including leukopenia and thrombocytopenia. It frequently requires prolonged hospitalization, and when the treatment is delayed due to misdiagnosis or in immunocompromized patients, HGE can be fatal (7). HGE is caused by infection with an obligatory intracellular bacterium, HGE agent. Comparison of 16 S rRNA gene sequences (2) and ultrastructure (8) indicates that the HGE agent is closely related to Ehrlichia phagocytophila, the agent of tick-borne fever, and Ehrlichia equi, the agent of equine ehrlichiosis. The HGE agent is transmitted by the Ixodes sp. tick (9), and the white-footed mouse is considered to be the major reservoir of the HGE agent in the United States (9).

Although the successful culture isolation of the organism was accomplished in 1995, little is known about the pathogenesis and intracellular parasitism of the HGE agent at the molecular level. Recent studies of the HGE agent showed that 38-49-kDa proteins of this organism are dominant antigens recognized by the sera from patients with HGE (10-13). We demonstrated that these proteins are present in the outer membrane fraction of our five human patient isolates of the HGE agent and of a tick isolate (USG3) of the granulocytic Ehrlichia (GE) sp. (12). More recently, we cloned, sequenced, and expressed in Escherichia coli a gene encoding a 44-kDa protein of the HGE agent (HZ isolate (8), also called isolate 13 (12-14)), and designated it p44 (13). Another ORF homologous to the p44 gene was found in the area downstream from the p44. This ORF lacked the universal start codon (AUG) and had two conserved sequences consisting of 59 and 65 amino acid residues identical to those of the p44 gene, which flanked a central hypervariable region. Genomic Southern blot analysis revealed more than 10 bands bound to the p44 gene probe, suggesting the existence of additional p44-homologous genes in the HGE agent genome. By Western blot analysis, a mouse antiserum against the recombinant P44 (rP44) protein recognized two to six P44-homologous proteins in each of the five isolates of the HGE agent and in USG3 isolate. Three monoclonal antibodies that react to the rP44 recognized one to four 44-kDa-range proteins in the whole organism as well as in the outer membrane fraction from these six isolates (14). Passive immunization with these antibodies induced a partial protection against infection with the live HGE agent in mice. These studies suggest that multiple antigenically cross-reactive proteins of 38-49 kDa are expressed in each isolate of the HGE and USG3 isolate in HL-60 cell culture and are potential protective antigens in HGE infection.

Murphy et al. (15) cloned three p44-homologous genes (msp-2a, msp-2b, and msp-2c) of USG3 isolate. The chemically determined amino acid sequences at N termini or internal segments of native 43- and 45-kDa proteins of the isolate approximately match with the segments of amino acid sequences predicted from one to two each of the three cloned genes. However, due to existence of highly conserved amino acid sequences among these P44-homologous proteins, whether these genes are actually expressed by the isolate was not conclusive in that study. Ijdo et al. (16) cloned a p44-homologous gene (hge-44) from the NCH-1 isolate of HGE agent and showed by RT-PCR and sequencing the product that this gene is expressed by the HGE agent in HL-60 cells.

So far, little is known about the relationship between the diversity of antigenically cross-reactive proteins of 38-49 kDa and multiple p44-homologous genes in the HGE agent. In this study, we characterized the structure, distribution, and expression of the p44-homologous genes of the HGE agent cultivated in HL-60 cells. Thereby, the study is expected to facilitate understanding of a role of the p44 multigene family in pathogenesis and immune responses in HGE infection and also to be helpful in designing a vaccine candidate by using these gene products.

    EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Culture and Purification of the HGE Agent-- The HGE agent (HZ isolate (8), also called isolate 13 (12-14)) was cultivated in HL-60 cells (human promyelocytic leukemia cell line) and purified by the Sephacryl S-1000 chromatography method as described elsewhere (17).

RT-PCR and Cloning of the cDNA-- A pair of oligonucleotides used for RT-PCR (p3708 and p4257 as shown in Fig. 1 and Table I) was designed based on the conserved regions between DNA sequences of the p44 gene and a truncated p44-homologous gene downstream from the p44. Total RNA was extracted from HL-60 cells infected with the HGE agent by using TRIzol reagent (Life Technologies, Inc.). The isolated RNA (3 µg) was heated at 70 °C for 10 min and reverse-transcribed in a 20-µl reaction mixture (0.5 mM deoxynuleoside triphophate mixture (dNTP), 200 units of SuperScript II reverse transcriptase (Life Technologies, Inc.), 2 pmol of p4257 primer, and 3 mM MgCl2) at 42 °C for 50 min. PCR was performed in a 100-µl reaction mixture containing 2 µl of the cDNA product, 10 pmol each of p3708 and p4257 primers, 0.2 mM dNTP mixture, 5 units of Taq DNA polymerase, and 1.5 mM MgCl2, with 3 min of denaturation at 94 °C followed by 30 cycles consisting of 1 min of denaturation at 94 °C, 1 min of annealing at 52 °C, and 2 min of extension at 72 °C. To rule out contamination of DNA in the RNA preparation, RT-PCR without reverse transcriptase was carried out (negative control). The amplified RT-PCR products were cloned in a pCRII vector by using the TA Cloning Kit (Invitrogen Co., San Diego, CA). Twenty-five cDNA clones, which were randomly selected from the transformants, were sequenced by dideoxy chain termination method with an Applied Biosystems 373 DNA sequencer.


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Fig. 1.   Primer positions in the p44 gene and the truncated ORF homologous to p44 in the recombinant plasmid pHGE1221 (13). Primers p3708 and p4257 were designed for RT-PCR to amplify the cDNA of p44-homologous genes. Primers hvf and hvr were designed to prepare the cDNA-specific probes by PCR for Southern and Northern blot analysis.

                              
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Table I
Oligodeoxynucleotides used in RT-PCR and PCR

Genomic Southern Blot Analysis-- DNA probes specific to each of the cDNAs for Southern blotting were designed based on a comparison of deduced amino acid sequences among these cDNAs. The central hypervariable regions (approximately 94 amino acid residues) in each cDNA and in the p44 gene were amplified by PCR with primer pairs as shown in Table I. The amplicons were cloned into a pCRII vector. The DNA insert excised from each recombinant plasmid was labeled with [alpha -32P]dATP by the random primer method with a kit (Amersham Pharmacia Biotech) and used as a probe. Hybridization was performed in rapid hybridization buffer (Amersham Pharmacia Biotech) as described elsewhere (13). The membrane was exposed to a Hyperfilm (Amersham Pharmacia Biotech).

Genomic Cloning of p44-homologous Genes of the HGE Agent-- The XbaI DNA fragments of the HGE agent, which were detected by genomic Southern blot analysis, were inserted into a pBluescript II KS (+) vector, and the recombinant plasmids were introduced into E. coli DH5alpha . By using the colony hybridization method (18) with the specific DNA probes same as those used for Southern blot analysis, three positive clones were isolated, and the DNA inserts were sequenced. The clones were designated pHGE3.0, pHGE3.4, and pHGE3.9, containing ehrlichial DNA fragments of 3.0, 3.4, and 3.9 kb, respectively.

Northern Blot Analysis-- Total RNA (15 µg) from the HGE agent-infected cells was separated on 1.2% denaturing agarose gel containing 0.22 M formaldehyde and transferred to Hybond-N+ nylon membrane (Amersham Pharmacia Biotech). The [alpha -32P]CTP-labeled single-stranded RNA probes (Table I) that were specific to each of cDNAs derived from the recombinant plasmids used for Southern blotting were prepared by using the riboprobe in vitro transcription system (Promega Corp., Madison, WI). The hybridization was performed separately with 1 × 107cpm/ml of each 32P-labeled RNA probe (a specific activity of 2 × 108 cpm/µg) in Prehyb/Hyb solution (Ambion, Inc., Austin, TX) at 65 °C for 16 h. After being washed twice each with low stringency solution and high stringency solution (Ambion) at 65 °C for 15 min, the membranes were exposed to a Hyperfilm. The densities of hybridized bands were analyzed with a PhosphorImager (Molecular Dynamics, Sunnyvale, CA).

Pulsed-field Gel Electrophoresis (PFGE)-- The purified HGE agent (25 µg of protein) was suspended in 10 mM Tris-HCl (pH 7.2), 20 mM NaCl, and 20 mM EDTA. The suspension was mixed with an equal volume of 2% CleanCut agarose (Bio-Rad) at 45 °C to make a sample plug for PFGE. The plug (5 × 5 mm) was incubated for 16 h at 50 °C in 100 mM EDTA (pH 8.0), 1 mg/ml proteinase K, and 1% sodium lauroyl sarcosine. After being washed twice with 20 mM Tris-HCl (pH 8.0), 50 mM EDTA containing 1 mM phenylmethylsulfonyl fluoride, the plug was washed twice in the same solution without phenylmethylsulfonyl fluoride. Then, the DNA in the plug was digested with 100 units of BamHI and EagI for 16 h at 37 °C and was electrophoresed in 1% agarose gel in 0.5× Tris borate-EDTA buffer (45 mM Tris, 45 mM borate, and 1 mM EDTA (pH 8.0)) by using CHEF DRIII (Bio-Rad) apparatus at 14 °C. Electrophoretic conditions were set at 6 V/cm with ramped pulse time from 1 to 5 s for 12 h, then from 1 to 10 s for 8 h, and finally from 10 to 15 s for 6 h. The DNA bands in a gel were visualized by ethidium bromide staining and transferred to nylon membrane. Southern blotting was performed with a mixture of five 32P-labeled probes specific to each of the cDNAs (p44-2 to p44-19 in Table I).

Estimation of Copy Numbers of p44-homologous Genes-- Two primer pairs, pnf12/pnr12 and pcf12/pcr12, (Table I) were designed based on both N- and C-terminal regions that had no homology each other but were conserved among the deduced amino acid sequences of p44-homologous genes. A pHGE 3.0 plasmid carrying a p44-12 gene (see Fig. 4) was used as a template for PCR. After PCR amplification with the primer pairs and the template, the amplicons were labeled with [alpha -32P]dATP and used as DNA probes. The probes were designated p44-12N (pnf12/pnr12) and p44-12C (pcf12/pcr12) as shown in Table I and Fig. 4. For Southern blot analysis, the genomic DNA of the HGE agent was digested with XbaI and PstI. The hybridization was done separately with the two 32P-labeled probes as described above. The densities of hybridized bands were analyzed by PhosphorImager. Two 3.0-kb XbaI fragments of the HGE agent that comigrated in a gel contained three p44-homologous gene copies (p44 and p44-18 in pHGE1221 and p44-12 in pHGE3.0 (see Fig. 4)). Consequently, the density of this 3.0-kb hybridized band in the Southern blot analysis represented three p44-homologous gene copies and was used as a standard to estimate the copy number.

Western Immunoblotting and Indirect Fluorescent Antibody (IFA) Labeling-- To examine the existence of p44-homologous gene products in the HGE agent organisms, Western immunoblot analysis and IFA were performed by using antisera against synthetic oligopeptides specific to each P44-homologous proteins. Briefly, by using DNASTAR program (DNASTAR Inc., Madison, WI), we analyzed the antigenic index, surface probability, and hydrophilicity and selected the unique amino acid sequence from the hypervariable region of P44-2 and P44-18 proteins. Two oligopeptides with sequences of GHSSGVTQNPKLFST and KNQKSSDTDTGVEKA were synthesized (Alpha Diagnostic, San Antonio, TX) and named Pep2 and Pep18, respectively (Fig. 2). Antisera against these synthetic oligopeptides were generated by immunization of a rabbit and a mouse with keyhole limpet hemacyanin (KLH) (Pierce)-conjugated synthetic oligopeptides Pep2 and Pep18, respectively. Western immunoblot analysis and IFA test with the above sera were performed as described elsewhere (14, 13). The mouse anti-recombinant P44 serum was used as a positive control. For double IFA staining, a lissamine rhodamine-labeled goat anti-rabbit IgG and fluorescein isothiocyanate-labeled goat anti-mouse IgG (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used as secondary antibodies.


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Fig. 2.   Alignment of amino acid sequences deduced from cDNA clones and the corresponding p44-homologous genes of the HGE agent. Aligned positions of identical amino acids with P44 of the HGE agent are shown with dots. Gaps indicated by dashed lines were introduced for an optimal alignment of all proteins. A boxed area in the middle indicates the amino acid sequences deduced from nucleotide sequences of cDNAs. Hypervariable regions are shown in boldface. A bar indicates the N-terminal amino acid sequence of the native P44 protein, and an arrowhead shows the cleavage site of the putative signal peptide. The amino acid sequences underlined in the hypervariable regions of P44-2 and P44-18 indicate the sequences that were used to prepare synthetic oligopeptides, Pep2 and Pep18, respectively. The arrows point out the positions of the primers used in RT-PCR. The numbers on the right indicate the positions of amino acid residues in P44-homologous proteins from the N terminus to C terminus.

Dot Immunoblot Assay-- The assay was carried out as described elsewhere (13). The KLH-conjugated oligopeptides Pep2 and Pep18 were used as antigens. Convalescent sera tested were from two patients (patients 7 and 11) with clinical signs of HGE; diagnosis was confirmed by IFA and PCR (12).

Sequence Analysis-- DNA and amino acid sequence analyses and phylogenetic studies were performed as described previously (19).

    RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

mRNAs Transcribed from p44-homologous Genes-- By RT-PCR, approximately 550-bp cDNAs were amplified from the total RNA of the HGE agent in HL-60 cells. The amplicon was observed as a single band in the gel by ethidium bromide, but no amplicon was detected without a reverse transcriptase (data not shown), indicating the absence of contamination of genomic DNA in the RNA preparation. After cloning the amplicons, only five different nucleotide sequences were found in the 25 randomly selected cDNA clones, and the deduced amino acid sequences are shown in Fig. 2 (boxed area). The five sequences of the cDNAs were represented as P44-2, P44-18, P44-12, P44-15, and P44-19. The numbers of cDNA clones with nucleotide sequences identical to P44-2, P44-18, P44-12, P44-15, and P44-19 were 12, 5, 3, 3, and 2, respectively (Table II). One or two substitutions were found at the 3'-end of three nucleotide sequences in a set of 12 cDNA clones represented by P44-2. We assume that these substitutions were generated by nucleotide misincorporation with reverse transcriptase or Taq polymerase. No cDNA sequence identical to the p44 gene was found in these 25 cDNA clones. This indicates that at least five different mRNAs from p44-homologous genes are transcribed by the HGE agent cultivated in HL-60 cell.

                              
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Table II
Properties of p44-homologous genes of the HGE agent

A comparison of the deduced amino acid sequences among the five different cDNAs and p44 gene previously cloned (13) revealed that a central region of approximately 94 amino acid residues corresponding to the 175th to 269th amino acid sequence of a protein (P44) encoded by the p44 gene was hypervariable, and the flanking regions of approximately 30 residues each were highly conserved (Fig. 2). Within the hypervariable region, the highest amino acid sequence similarity was 32.8%, between the P44-2 and P44-18 proteins, and the lowest similarity was 19.9%, between P44-15 and P44 proteins. In comparison with flanking regions, the hypervariable region had higher hydrophilicity and antigenic index.

Southern Blotting and Genomic Cloning of the p44-homologous Genes Expressed by the HGE Agent-- By using a DNA probe specific to each of cDNAs of P44-18, P44-12, P44-19, and the p44 gene, a single DNA band was detected in all restriction digestions tested (Fig. 3). Because restriction enzymes used do not cut within a p44 gene or any cDNA clones, this suggests that each gene corresponding to the four probes is a single copy in the HGE agent genome. However, the probes specific to the cDNAs of P44-2 and P44-15 generated two or three bands (Fig. 3), showing that two or three gene copies with sequences identical or highly homologous to those of the cDNAs of P44-2 or P44-15 exist in the HGE agent genome. Hybridization patterns with the probes specific to P44-18 cDNA and the p44 gene were identical to each other in the four restriction digestions. This is consistent with the result of our sequence analysis data that the p44 gene and a p44-homologous gene (p44-18) without a universal start codon (AUG) were overlapped by 21 bp in an XbaI fragment in pHGE1221 plasmid (Fig. 4). Overall, at least nine copies of p44-homologous genes (a total of the copies of genes identified in Table II) either expressed and/or highly homologous were detected in the genome by using cDNA- specific probes.


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Fig. 3.   Genomic Southern blot analysis of the HGE agent with the DNA probes specific to each of five cDNAs and p44 gene. Probes are shown at bottom of each panel. Hybridization was performed at 65 °C for 16 h, and the membrane was washed twice with 0.1× SSC (1× SSC, 0.15 M sodium chloride and 0.015 M sodium citrate) and 0.1% SDS at 60 °C for 30 min. Numbers on the left indicate molecular sizes in kb.


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Fig. 4.   Schematic diagram of the DNA inserts of recombinant plasmids pHGE1221, pHGE3.9, pHGE3.0, and pHGE3.4. The arrow indicates the orientation of ORF from 5' to 3' end.

Three DNA fragments of 3.0, 3.4, and 3.9 kb were cloned from the XbaI-digested genomic DNA of the HGE agent with the probes specific to the cDNAs of P44-2, P44-12, and P44-15 (Fig. 4). Sequencing of the 3.9- and 3.0-kb fragments revealed two complete ORFs of 1275 and 1173 bp encoding 425- and 391-amino acid proteins with molecular weights of 44,969 and 41,179, respectively. As expected, these ORFs (p44-2 and p44-12) contained the sequences identical to the cDNAs of P44-2 and P44-12, respectively (Figs. 2 and 4). In the 3.9-kb DNA, an additional small ORF (p44-c), which had a DNA sequence corresponding to 82 amino acid residues (9062 Da) at the C terminus of the P44-2 protein, was found at 111 nucleotides downstream from the p44-2 gene. The 3.4-kb DNA fragment contained an ORF of 834 bp encoding a 278-amino acid protein with a molecular weight of 29,387. This ORF (p44-15) included a sequence identical to P44-15 cDNA. The p44-15 did not have a universal start codon and lacked DNA sequence corresponding to 82 amino acid residues at the N terminus of the P44 protein. Consensus sequences of sigma 70 promoter (AT-rich region about 10 base pairs upstream of the transcription start site; the -10 sequence) and Shine-Dalgarno sequence were found in the regions upstream from the start codon of p44-2, p44-12, and p44-c. The properties of the p44-homologous genes identified in this study were summarized in Table II.

The N-terminal amino acid sequence (HDDVSALETG) of the native 44-kDa protein previously determined (13) was also found in P44-2 and P44-12 proteins as shown in Fig. 2. A comparison of deduced amino acid sequences of these cloned genes suggests that the identical amino acid sequence consisting of 15 residues at the N terminus of P44, P44-2, or P44-12 protein is a signal peptide. The alignment also showed that the N- and C-terminal portions are highly conserved among three P44 homologs (P44-2, P44-12, and P44), except for the existence of additional 34 amino acid residues at C terminus of P44-2 (Fig. 2). At N and C termini of both P44-15 and P44-18, proteins that are encoded by the ORFs without universal start codons had a short stretch of an amino acid sequence consisting of 8-28 residues without any homology to other P44 homologs, including P44, P44-2, and P44-12 (Fig. 2).

Northern Blot Analysis-- To confirm whether the relative proportion of the cDNA clones represented different levels of transcription of the p44-homologous genes and to determine the transcript sizes, the Northern blotting was performed with three RNA probes. The probe specific to P44-2 and P44-18 cDNAs strongly hybridized with the respective 1.5-kb mRNA transcript (Fig. 5). Using same amounts of total RNA and each probe, the density of band hybridized with P44-2-specific probe was at least 2-fold greater than that with P44-18-specific probe. No hybridized band was observed when p44-specific probe was used. These findings support the cDNA cloning results that, among the p44-homologous genes, p44-2 was more abundantly expressed in the HGE agent than p44-18, and the p44 gene was silent.


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Fig. 5.   Northern blot analysis of the mRNA expression of the p44-homologous genes. The single-stranded RNA probes specific to cDNAs of P44-2 and P44-18 and the p44 gene are shown at bottom of the lanes. Numbers on the left indicate molecular size in kb.

Expression Sites of p44-homologous Genes in the HGE Agent Genome-- In a PFGE gel, EagI and BamHI digestions produced 14 and 23 bands ranging from 9 to 145 kb and from 2 to 194 kb, respectively (data not shown). In the Southern blotting (Fig. 6), the probes hybridized to larger fragments of 100, 97, 49, and 12 kb in EagI digestion and 50, 20 and 18 kb in BamHI digestion. These results show that these five different expressed genes did not form a cluster in the genome of the HGE agent.


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Fig. 6.   Distribution of expressed p44-homologous genes in the HGE agent genome. Southern blot analysis after PFGE was performed by using a mixture of DNA probes specific to the cDNAs of P44-2, P44-12, P44-15, P44-18, and P44-19. Hybridization was performed at 65 °C for 16 h, and the membrane was washed twice with 0.1× SSC and 0.1% SDS at 60 °C for 30 min. Numbers on the right indicate molecular sizes in kb.

Copy Number of p44-homologous Genes-- In the genomic Southern blot analysis (Fig. 7), the hybridization patterns of major bands with p44-12N and p44-12C probes (no homology each other) in two restriction digestions were almost identical to each other, except that two restriction fragments of 2.2 and 1.5 kb in XbaI and three restriction fragments of 2.0, 1.7, and 1.2 kb in PstI hybridized only with either p44-12N or p44-12C probes (Fig. 7, asterisks). This suggests that two and three copies of p44-homologous genes lack the 3'- or 5'-end conserved regions, respectively. The restriction sites of these enzymes were not found in the DNA sequences of nine p44-homologous genes so far cloned (five in the present study, three in Ref. 15, and one in Ref. 16). However, one or two p44-homologous genes that have not been cloned may be digested by the restriction enzymes. Because any sequence divergences between the probes and target p44-homologous genes reduce the hybridization signal, ratios of densities of the bands to the 3.0-kb band approximately represent a minimum copy number of p44-homologous genes in each of these hybridization bands. Therefore, we concluded that there are at least 18 of p44-homologous genes in the HGE agent genome. Because by genomic Southern blotting with cDNA-specific probes, only nine copies of p44-homologous genes were detected (Fig. 3 and Table II), at least nine more copies with sequences in their hypervariable regions divergent from any of those expressed genes appear to exist in the HGE agent genome.


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Fig. 7.   Estimation of minimum copy numbers of p44-homologous genes in the genome of the HGE agent. A, Southern blotting profiles by using the DNA probe specific to P44-12 cDNA and the DNA probes of p44-12N and p44-12C that were derived from N-terminal and C-terminal regions of p44-12, respectively. Each probe is shown at bottom of the respective lane. Hybridization was performed at 60 °C for 16 h, and the membrane was washed twice with 0.1× SSC at 60 °C for 30 min. The asterisks indicate that the unique restriction fragments recognized only by either p44-12N or p44-12C but not by both. Numbers on the left indicate molecular sizes in kb. B, schematic illustration of blotting patterns for the copy number analysis. The numbers on the right indicate a ratio of the band density against that of the 3.0-kb band in XbaI digestion.

Analysis of HGE Agent Organisms Expressing P44-homologous Proteins-- The rabbit anti-Pep2 and the mouse anti-Pep18 sera reacted with a single band of 44 and 43 kDa, respectively, in the HGE agent lysate (Fig. 8). In agreement with our RT-PCR and Northern blot results, the size of the protein recognized by the anti-Pep18 serum was not 26.4 kDa, as was predicted from the 759-bp ORF of the p44-18 gene. The result of Southern blotting with p44-18-specific probe ruled out the possibility of the existence of other expression loci for p44-18 in the genome of the HGE agent. Therefore, all these results suggest that transcriptional modification, such as splicing, is involved in the expression of p44-18. Mouse antibody against a recombinant P44 protein (rP44) that is expected to detect most of p44-homologous gene products because the rP44 protein includes N-terminal region highly conserved among P44 homologs (13), predominantly reacted with two proteins of 44 and 43 kDa (Fig. 8). The anti-rP44 serum was used as a positive control to visualize most organisms expressing P44 homologs by a double immunofluorescence labeling (Fig. 9). In host cells, the HGE agent resides in membrane-bound inclusions that appear as clusters of organisms that resemble mulberries and are therefore called morulae. The morulae are considered as microcolonies derived from a single organism. As shown in Fig. 9 (arrowheads), six morulae in an HL-60 cell that reacted with the mouse anti-rP44 serum were also recognized by the rabbit anti-Pep2 serum. All organisms were double-labeled in 100 infected cells scored in three independent labeling experiments. This means that a p44-2 gene is probably expressed in all of the HGE agent organisms cultivated in HL-60 cells. With a mouse anti-Pep18 serum, even at a low (1:5) dilution, the immunofluorescence labeling of organisms or morulae was extremely weak (data not shown), although Fig. 8 showed that a 43-kDa protein of the HGE agent was strongly recognized with the serum at 1:400. The reason may be that the epitope(s) of this oligopeptide is masked or sterically hidden in the HGE agent organism. Based on these results, the existence of mRNA for five p44-homologous genes, and the relative proportion of cDNA clones, we suggest that multiple p44-homologous genes are differentially expressed by each organism. Accordingly, HGE agents cultivated in HL-60 cells probably consist of a heterogeneous population of organisms expressing one, two, or multiple p44-homologous gene products.


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Fig. 8.   Western blot analysis of rabbit anti-Pep2, mouse anti-Pep18, and mouse anti-rP44 sera using the purified HGE agent. Ten µg of proteins of the purified organisms were used as an antigen in each lane. The three primary antisera were used at 1:400 dilution. The numbers on the left indicate molecular masses in kDa based on the broad-range prestained standards (Bio-Rad).


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Fig. 9.   Double immunofluorescence labeling of the HGE agent with rabbit anti-Pep2 and mouse anti-rP44 sera. The binding of each antibody was detected by using lissamine rhodamine-labeled goat anti-rabbit IgG and fluorescein isothiocyanate-labeled goat anti-mouse IgG. Fluorescein isothiocyanate-labeled (right) and lissamine rhodamine-labeled (left) morulae containing multiple HGE agent organisms are shown by arrows in the paired photomicrographs. Magnification, × 750.

Detection of Antibodies Specific to P44-2 and P44-18 Proteins in the Sera of Patients with HGE Infection-- Whether antibodies against P44-2 and P44-18 proteins were present in convalescent sera from patients with HGE was examined by dot immunoblot assay with the oligopeptides of Pep2 and Pep18 as antigens (Fig. 10). Serum 7 reacted strongly to both Pep2 and Pep18. Serum 11 also reacted strongly to Pep18 but weakly to Pep2. This result indicates that p44-2 and p44-18 genes are actually expressed by the HGE agent in infected patients; thus, antibodies specific to these P44 homologs were developed.


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Fig. 10.   Dot immunoblot assay of HGE patient sera (patients 7 and 11) with oligopeptide Pep2 and Pep18. KLH-conjugated Pep2 and Pep18, and KLH as a negative control, were blotted onto the nitrocellulose membrane at 200 ng of protein per dot. The patient sera were preabsorbed with KLH and used at a 1:300 dilution.

Phylogenetic Analysis-- A phylogenetic analysis revealed nine P44 homologs had identities of 58.3-99.5% (Table III) and made a cluster separated from two major surface protein 2 (MSP2) (2-11.2 and 2-DF (20, 21)) and a MSP4 (22) of Anaplasma marginale (Fig. 11). The result is in agreement with phylogenetic analysis based on 16 S rRNA sequence comparison. This also indicates that P44 homologs of HGE and USG3 isolates were not segregated either by geography (e.g. New York versus Massachusetts) or host origin (e.g. human versus tick).

                              
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Table III
Identities and evolutionary distances among amino acid sequences predicted from entire genes of HGE agent P44 and its homologs


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Fig. 11.   Phylogenetic classification among the proteins of P44 family and other major outer membrane proteins of the closely related rickettsiae based on the amino acid sequence similarities. Scale bar shows 10% divergence in amino acid sequences. The GenBankTM accession numbers of the published sequences are as follows: P44 (HGE agent, HZ strain), AF059181; HGE-44 (HGE agent, NCH-1 strain), AF037599; MSP-2A (GE sp., USG3 strain), AF029322; MSP-2B and -2C (GE sp., USG3 strain) and MSP4 (A. marginale, Florida strain), Q07408; MSP2-11.2 (A. marginale, Florida strain), U07862; and MSP2-DF (A. marginale, Florida strain), U36193.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

This study demonstrated that multiple p44 genes are expressed by the HGE agent in HL-60 cells and probably in patients, which may be the reason why multiple bands of 38-49 kDa were found in our previous Western blot analysis studies with patients' sera (12) or with monoclonal antibodies (14). Our data set the framework for a better understanding of the mechanism(s) underpinning the expression of p44 multigenes in the HGE agent. Four of expressed p44-homologous genes (p44-2, p44-12, p44-15, and p44-18) and one silent gene (p44) from the genomic DNA were cloned. The proteins encoded by these p44-homologous genes were found to consist of a single central hypervariable region of approximately 94 amino acid residues and N- and C-terminal regions highly conserved among the homologs. It appears that two kinds of mechanisms may involve in the expressions of p44-homologous genes: (i) the normal expression of the genes, such as p44-2 and p44-12, with complete ORFs from the respective expression sites, and (ii) the unique expression with a specific event, probably transcriptional modification such as splicing, of the genes, such as p44-18 and p44-15, which lack a universal start codon. The latter mechanism may come to existence to overcome the deficiency in creating antigenic diversity by a common mechanism, such as recombination.

p44 multigenes are homologous to two A. marginale msp2 genes that had been cloned (~ 45% amino acid similarity (13, 15, 16)). Recently, the study on A. marginale revealed that multiple (at least 4) msp2 genes were expressed in each peak of rickettsemia that occurred at 6-8-week intervals in two cattle (20). The distribution of the genes in the genome (broad distribution throughout the genome) and the protein structure (e.g. a central hypervariable region flanked with conserved regions) are similar between msp2 genes and p44 multigenes. Because the copy number of msp2 multigenes in A. marginale was estimated as 10 (21), the relative ratio (4 in 10) of expressed genes against the total copy number of msp2 multigenes at a given stage of infection is higher than that (5 in 18) of p44 multigenes of the HGE agent. In other words, the HGE agent may have more potential genetic capacity to generate the diversity of the P44-homologous proteins. Because for A. marginale, the msp2 genes corresponding to the transcripts were not identified, the expression mechanism is unknown.

Among rickettsia closely related to HGE agent, persistent infection and recurrence after recovery from the clinical disease are known for Ehrlichia canis, Ehrlichia platys, E. phagocytophila, Cowdria ruminantium, and A. marginale (20, 23). The HGE agent was detected in the serum of one untreated patient by PCR at 30 day after onset of illness (24), suggesting that the HGE agent may also cause persistent infection in humans. In addition, persistence of the HGE agent in reservoir rodent host would be an important adaptation that allows greater access of uninfected tick populations to an infectious blood meal. French et al. (20) proposed that the expression of distinct sets of msp2 genes at each rickettsemia peak in A. marginale infection of cattle may allow immunoevation of anaplasma to persist in immunocompetent hosts. Multiple expression of p44 multigenes may be also related to immunoavoidance in human and rodent hosts and potential persistence.

The expression mechanisms of the several multigene families in the human pathogens Neisseria gonorrhoeae (pil (25) and opa (26)), Borrelia hermsii (vmp (27)), Borrelia burgdorferi (vls (28)), African trypanosomes (vsg (29)), and Plasmodium falciparum (var (30)), have been well studied. These multigene families are involved in pathogenesis, e.g. antigenic variation (or phase variation) and cytoadhesin. The switching of gene expression can be divided into two major mechanisms: one depends on DNA rearrangement, and another occurs at transcriptional level. In pil, vmp, vls, and vsg, switching of expression between members of the corresponding gene families occurs through programmed DNA rearrangements (gene conversion), moving a transcriptionally silent gene into an active expression site. In opa, expression is regulated by a reversible frameshift mutation of DNA, i.e. a slipped-strand mispairing of the number of pentanucleotide coding repeat units in the single peptides (26), whereas in the var gene of P. falciparum, each parasite expresses only a single and distinct var gene product. Each var gene is an independent transcription unit in which promoter activity determines the expression status. p44 multigene expression appears to be different from those of pil, vmp, vls, and vsg in that multiple expressed p44 genes are located in several large DNA fragments in the HGE agent genome, i.e. the transcription of the genes apparently does not occur from a unique expression site. The expression p44 multigene, therefore, may not involve DNA rearrangement. Expressions of p44 multigenes, as well as msp2 genes (21), are also different from that of var genes, because a single organism appear to express more than two gene products.

In ehrlichiae, recently, additional multigene families encoding major outer membrane proteins have been discovered. We identified the omp-1 gene family of Ehrlichia chaffeensis (31) and the p30 gene family of E. canis (19). More than a dozen copies of the omp-1 and p30 gene families are tandemly arranged in the genome, in contrast to p44 multigenes and msp2 genes. Furthermore the protein structures encoded by the omp-1 and p30 gene families is distinct from those of p44 and msp2, consisting of three short, hypervariable segments interposed with relatively conserved segments (19, 31). This suggests that the expression mechanism of omp-1 and p30 gene families is probably different from those of p44 and msp2. Immunization with a recombinant P28 protein (one of the omp-1 multigene products) of E. chaffeensis and native MSP2 of A. marginale has been demonstrated to induce almost complete and partial protections against the infection in mice and cattle, respectively (31, 32). We previously observed that passive immunization with monoclonal antibodies specific to P44-homologous proteins of the HGE agent induced partial protection against challenge with the HGE agent in mice (14). Active immunization with the recombinant P44 protein also partially protected mice from HGE infection.2 This indicates that the p44 multigene family encodes a potential protective antigen. However, because only a fraction of genes is differentially expressed as shown in this study, it seems to be essential to determine which genes are expressed in the host for identification of the most protective antigen.

By a GenBankTM data base homology search, the P44-homologous proteins of the HGE agent were found to possess a similarity (~64%) with the N-terminal region of Hsf protein (surface fibrils) involved in binding of Hemophilus influenzae type b to human epithelial cells (33). The similar sequence identified was located in the center of all P44 homologs, including the hypervariable domain (span of approximately 200 amino acid residues). This finding suggests that P44s may have a similar function, such as cytoadhesion to human granulocytes.

The basic information obtained in this study would facilitate the understanding of the role of the p44 multigene family in causing disease in humans, in the persistence of HGE agent in white-footed mice, and in transmission of this agent from tick to human. Further analysis of expression mechanism of these genes and the genes encoding more protective antigens would assist in designing an effective vaccine candidate against the human ehrlichiosis.

    FOOTNOTES

* This work was supported by National Institutes of Health Grant RO1 AI40934.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF135254, AF135255, AF135256, AF135257, AF135258, AF135259, AF132260, AF132261, AF132262, and AF132263.

Dagger To whom correspondence should be addressed: Dept. of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Rd., Columbus, OH 43210-1093. Tel.: 614-292-9677; Fax: 614-292-6473; E-mail: rikihisa.1{at}osu.edu.

2 N. Zhi, N. Ohashi, and Y. Rikihisa, unpublished data.

    ABBREVIATIONS

The abbreviations used are: HGE, human granulocytic ehrlichiosis; RT, reverse transcription; PCR, polymerase chain reaction; IFA, indirect fluorescent antibody; KLH, keyhole limpet hemacyanin; PFGE, pulsed-field gel electophoresis; ORF, open reading frame; kb, kilobase(s); bp, base pair(s); rP44, recombinant P44.

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
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