Interaction Mapping of a Dynein Heavy Chain
IDENTIFICATION OF DIMERIZATION AND INTERMEDIATE-CHAIN BINDING DOMAINS*

Andrea HaburaDagger , Irina TikhonenkoDagger , Rex L. Chisholm§, and Michael P. KoonceDagger parallel

From the Dagger  Division of Molecular Medicine, Wadsworth Center, Empire State Plaza, Albany, New York 12201-0509, the § Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611-3072, and the  Department of Biomedical Sciences, State University of New York, Albany, New York 12201-0509

    ABSTRACT
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Cytoplasmic dynein is a multisubunit microtubule-based motor protein that is involved in several eukaryotic cell motilities. Two dynein heavy chains each form a motor domain that connects to a common cargo-binding tail. Although this tail domain is composed of multiple polypeptides, subunit organization within this region is poorly understood. Here we present an in vitro dissection of the tail-forming region of the dynein heavy chain from Dictyostelium. Our work identifies a sequence important for dimerization and for binding the dynein intermediate chain. The core of this motif localizes within an ~150-amino acid region that is strongly conserved among other cytoplasmic dyneins. This level of conservation does not extend to the axonemal dynein heavy chains, suggesting functional differences between the two. Dimerization appears to occur through a different mechanism than the heavy chain-intermediate chain interaction. We corroborate the in vitro interactions with in vivo expression of heavy chain fragments in Dictyostelium. Fragments lacking the interaction domain express well, without an obvious phenotype. On the other hand, the region crucial for both interactions appears to be lethal when overexpressed.

    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Dynein is a microtubule-associated motor protein that is responsible for several distinct motility processes in eukaryotic cells (reviewed in Refs. 1-3). The dynein superfamily is divided into two major classes based primarily on their cellular function. Cytoplasmic dyneins act in positioning and transport of membrane-bound organelles and help organize spindle assembly during cell division. The more heterogeneous axonemal dyneins are largely responsible for the beating motions of eukaryotic cilia and flagella. All dyneins characterized to date are composed of multiple subunits. A complete dynein molecule typically contains two or three heavy chains (DHC)1 with molecular masses of >400 kDa; intermediate chains (DIC) of 70-80 kDa, some of which have been localized to the dynein tail (4, 5); and several light chains of different molecular masses (1, 3, 6-8). Many cytoplasmic forms also contain at least two light-intermediate chains (50-60 kDa) (9, 10).

Comparisons between axonemal and cytoplasmic dyneins suggest a functional division of the DHC into two regions. The carboxyl-terminal two-thirds is conserved and comprises the motor domain (11-14). The more divergent amino-terminal third forms at least part of the stalk and likely forms a scaffold upon which most of the other subunits assemble to form the cargo-binding tail (1, 11, 15, 16). The molecular interactions within the dynein tail are not well understood. They are important, however, because variation in the subunit composition is thought to specify and regulate cargo binding either directly or by controlling the interaction of dynein with other proteins such as dynactin (16-20).

Our work in Dictyostelium has shown that overexpression of the motor domain in this organism dramatically alters the interphase microtubule array, but the cells are viable (13). In contrast, a fragment containing the amino-terminal region can only be expressed at low levels; its overexpression appears to be lethal (21). Because this fragment can associate with the native DHC in vivo (21), its overexpression may cause the formation of one-headed or headless dyneins that can bind cargo but cannot function as motors (see also Ref. 22).

To further investigate the toxic effect of this dynein fragment, and characterize its domain structure, we have initiated a functional dissection of the DHC amino terminus from Dictyostelium. We identify here a highly conserved region that is important for DHC homodimerization and for intermediate chain binding. Both these interactive properties are confined to the same region of the DHC; however, they have different sequence requirements and are biochemically distinct in vitro. We further correlate these results with expression of similar regions of the DHC in vivo. This is the first identification of a subunit-interaction domain in a cytoplasmic dynein heavy chain, and it provides a target for investigations into the structure and assembly dynamics of the dynein tail.

    EXPERIMENTAL PROCEDURES
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Construction of Vectors-- A fragment containing the amino-terminal 4007 base pairs of the Dictyostelium DHC cDNA was subcloned into the pET5 expression vector (Novagen) just downstream of the T7 transcription initiation site. Smaller amino-terminal fragments were generated by endo- and exonuclease digestion and cloned into pET5 or pRSET (Invitrogen) expression vectors (see Fig. 5 for summary).

A construct encoding the full-length Dictyostelium DIC cDNA (652 aa, GenBankTM accession number U25116 (23)), subcloned into pET 14 (Novagen), was used for the initial stages of this work. However, we found that a large EcoRI fragment of the DIC cDNA (aa 65-613) shows the same binding activity as the full-length DIC but is produced in greater amounts by BL21(DE3) cells. It is used almost exclusively in the data presented here. Plasmids were purified according to the alkaline lysis/polyethylene glycol method (24).

Construction of Affinity Resins-- Expression vectors encoding the DHC amino-terminal fragment and the full-length and partial DIC cDNAs were expressed in BL21(DE3) cells. Polypeptides were purified from inclusion bodies following the methods of Lin and Cheng (25). Inclusion bodies were solubilized in renaturing buffer (50 mM Tris, 500 mM NaCl, 0.05% Tween 80, 20% glycerol, pH 7.6) containing 8 M urea. Soluble protein was clarified at 25,000 × g for 20 min and adjusted to a concentration of 2 mg/ml. This solution was dialyzed stepwise at 2-h intervals against 25 volumes of buffer with decreasing concentrations of urea (4, 2, 0 M), then against CNBr coupling buffer (100 mM NaHCO3, 500 mM NaCl, pH 8.3) overnight, and clarified at 25,000 × g for 20 min. The concentration of soluble protein was determined by the Bradford assay, and the protein was then coupled to one-fourth volume activated CNBr resin (Amersham Pharmacia Biotech) overnight. This resulted in a final protein concentration on the resin of ~3 mg/ml. The affinity resin was blocked with 1 M glycine or 100 mM Tris, pH 8, washed, and stored in PHEM buffer (60 mM Pipes, 25 mM HEPES, 8 mM EGTA, 2 mM MgCl2, pH 6.9 (26)) containing 0.02% sodium azide on ice.

Affinity Analysis of Reticulocyte Lysate-expressed Proteins-- DHC constructs were expressed in vitro using the TnTTM reticulocyte lysate system (Promega). 50 µl of the reaction mixture was brought to 500 µl with PHEM + 250 mM NaCl (PHEM 250). This concentration of NaCl reduces nonspecific interactions between protein and resin but does not disrupt the subunit organization of native cytoplasmic dynein (not shown). The diluted lysate was clarified at 18,000 × g for 20 min and divided equally into two or three aliquots. Each aliquot was brought to 1 ml with PHEM 250 and incubated with 50 µl of the appropriate affinity or control resin at 4 °C for 2-4 h. The resin was washed three times with 1-ml volumes of PHEM 250, resuspended in an equal volume of 4× SDS-PAGE sample buffer, and boiled. Bound polypeptides were resolved by electrophoresis through a 7.5% SDS-polyacrylamide gel. Reticulocyte-lysate expressed bands were detected by soaking the gel in Amplify (Amersham Pharmacia Biotech), drying, and exposing to film. The amount of [35S]methionine-labeled polypeptide was determined using a Fujix phosphoimage BAS 2000 scanner (Fuji). Bands were selected and analyzed with TINA analysis software (Raytest) using the Autocontour feature.

Cross-linking of Reticulocyte Lysate-expressed Proteins-- DHC amino-terminal fragments were expressed in vitro as described above. The sample was divided into two or three aliquots and brought to 50 µl by addition of buffer containing 4 µl of DSP stock in N,N-dimethylformamide (final concentration 0.8 mg/ml), EDC stock in H2O (final concentration 0.16 mg/ml), or addition of N,N-dimethylformamide or water as control. Samples were incubated at room temperature for 30 min, and the reaction was stopped with 5 µl of 1 M ethanolamine. Samples were brought to 500 µl with PHEM 250, clarified at 18,000 × g for 20 min, and then incubated with antibodies raised against the DHC amino terminus (27) bound to Protein A-Sepharose (Amersham Pharmacia Biotech). The immunoprecipitate resin was then washed three times, and bound polypeptides were resolved by SDS-PAGE.

Statistical Methods-- The data from the DHC-DHC and DHC-DIC affinity resin experiments were examined using a cluster analysis (28). A set of 1000 distributions was generated by randomly assigning a fragment to one of two groups, and a mean group affinity was calculated. For each distribution, the mean squared error from the group mean was calculated for every fragment. The randomly generated distribution that had the lowest G statistic (defined as the sum of all mean squared errors) was designated as "best" and was used as the basis for designating fragments as either binding or nonbinding.

Expression of Polypeptides In Vivo-- Amino-terminal constructs were tagged by oligonucleotide addition of the c-Myc epitope (29) (details available upon request). These were subcloned downstream of the native DHC promoter on a plasmid containing a G418 selection marker (13, 21). Fresh AX-2 cell spores were plated onto 10-cm plastic Petri dishes and were transformed with plasmid DNA (13, 21) just before the cells reached confluency, at about 107 cells/dish. Following an overnight recovery, the medium was changed to include 10 µg/ml G418. Colonies were detected after 3-5 days of drug selection. Individual clones were picked with a pipette and transferred to a 24-well plate.

Biochemical Analysis of Dictyostelium Proteins-- Preparation of high speed supernatants (HSS), electrophoresis, and immunoblotting were performed as described in (30). c-Myc-tagged polypeptides were purified from HSS by incubating 1 ml of supernatant with 25 µl of anti-c-Myc monoclonal antibody from ascites for 1 h at 4 °C. 25 µl of Protein A-Sepharose (Amersham Pharmacia Biotech) was then added to bind the antibodies. The resin was washed three times and resuspended in 50 µl of PHEM buffer. Bound polypeptides were resolved by SDS-PAGE.

    RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Cross-linking of the DHC Amino Terminus-- Previous work has suggested that the heavy chains of cytoplasmic dynein associate directly in the absence of other dynein subunits (31). Because the two heavy chains are believed to interact within the amino-terminal third of the DHC, we tested whether this region can dimerize in vitro. A reticulocyte lysate expressing the amino-terminal 1327 aa (151 kDa) of the DHC was exposed to either DSP or EDC cross-linkers to covalently link polypeptides in close contact with one another. DSP is approximately 8 Å in length and cross-links the epsilon -amine of lysine residues; EDC is a zero-length cross-linker that forms a covalent bond between aspartate or glutamate and lysine. As indicated in Fig. 1, both cross-linkers produced labeled bands of MW ~300 kDa, a value close to the predicted dimer weight of the amino-terminal fragment.


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Fig. 1.   Cross-linking of DHC amino terminus. A reticulocyte lysate-expressed 1327-aa DHC amino-terminal fragment was incubated with the cross-linkers DSP, EDC, or with N,N-dimethylformamide (DMF) as a control. Protein was immunoprecipitated with anti-DHC antibody bound to Protein A-Sepharose. Both cross-linkers generate a band of approximately 300 kDa, the predicted dimer size of the 151-kDa fragment. No other high molecular weight bands are visible, even after longer exposures.

Purification of DHC Amino Terminus with DHC Affinity Resin-- To further investigate DHC dimerization, a construct encoding the amino-terminal 1327 aa of Dictyostelium DHC was expressed in Escherichia coli (see Fig. 2A) and coupled to Sepharose, producing a DHC affinity resin. Two types of control resins were constructed to measure nonspecific interactions: non-dynein proteins (actin or BSA) bound to Sepharose at the same concentration, and a similarly-treated protein-free resin.


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Fig. 2.   Binding of the dynein amino terminus to affinity resin. A, lane 1 shows a Coomassie Blue-stained gel of the purified inclusion bodies that were used to construct the amino-terminal 151-kDa DHC affinity resin. Lane 2 shows an immunoblot of the same sample probed with a polyclonal DHC antibody. B, DHC amino terminus interaction with DHC affinity resin. Equal amounts of reticulocyte-expressed polypeptide (DHC aa 1-1327) were incubated with DHC affinity resin and with a control resin, either BSA, actin, or protein-free-Sepharose (Seph). Luciferase (luc), a control protein, was similarly expressed and incubated with either DHC or BSA affinity resin. This panel shows autoradiographs of material retained by the resin after washing. DHC affinity resin retains significantly more DHC than do any of the control resins (there were no statistical binding differences between the control resins). In contrast, the luciferase control bound with the same low affinity to the DHC as to control resin. C, the amount of radiolabel in each gel band was quantitated with a phosphoimager. This panel shows the mean ratio of protein bound to the DHC affinity resin versus control resin for the 1-1327 DHC and luciferase. DHC resin retains approximately 3-fold more DHC than do the control resins; luciferase binds equally well to the DHC affinity and BSA resin.

Incubation of the DHC affinity resin with the same, in vitro expressed polypeptide showed an approximately 3-fold higher retention of the soluble fragment than on control resins (Fig. 2, B and C). This suggests that the bound and soluble fragments interact in the assay. The resin-bound DHC fragment does not retain protein indiscriminately; luciferase does not bind to the affinity resin any more strongly than to the control resins. Moreover, the resins do not retain any of the more abundant protein components of the reticulocyte lysate at levels detectable by Coomassie Blue staining (not shown).

Dimerization Capabilities of DHC Subunits-- To determine a minimal HC fragment that supports dimerization, we produced a series of smaller fragments (summarized in Fig. 5) and tested their ability to interact with the DHC resin and to cross-link. As expected, the fragments showed different affinities for DHC resin (Fig. 3A). Specific retention was measured as the ratio of band intensity on the affinity resin to its relevant control. These ratios fell into two groups, suggesting a defined binding site which some, but not all, constructs contain. One group comprises DHC fragments that bind more than twice as efficiently to affinity resin than to control resin; these were considered to contain the binding site. In all cases, these polypeptides bound to DHC resin at least as well as did the full-length DHC amino terminus. The second group consists of those that exhibit a mean affinity resin:control resin intensity ratio of less than 2; these were considered noninteractors. Such low ratios are similar to the nonspecific behavior exhibited by luciferase in this system (see Fig. 2B). Statistical analyses showed that, relative to the stated null distribution, these results are significant (P value of 0.04). All of the constructs that showed binding activity contain the region between aa 627 and 780. No fragment lacking this region showed binding ability. However, this region alone is not sufficient for dimer interaction in vitro.


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Fig. 3.   Fragments of DHC amino terminus exhibit distinct DHC binding abilities. A, the autoradiograms in the upper panel show representative pairs of several amino-terminal fragments (encoded amino acids are listed below each pair; see Fig. 5 for a summary) bound to the DHC affinity resin and a control resin. The 1-1327 and luciferase samples are the same as shown in the previous figure and are provided here for reference. The lower panel provides the mean relative affinity of the DHC resin versus control resin from multiple independent experiments. The black bars represent fragments statistically identified as interactors; the white bars represent noninteractors. B, three examples of DSP cross-linking experiments. The panels show autoradiograms from reticulocyte lysate-expressed and immunoprecipitated samples following SDS-PAGE. + or - indicates whether DSP was added as a cross-linker. Constructs 1-565 and 780-1327 show no evidence of higher molecular weight bands as a result of adding cross-linker; fragment 629-1337 (predicted mass of 81 kDa) shows a band of approximately 160 kDa (arrow) in the presence of DSP. This work is summarized in Fig. 5.

These fragments also displayed differential cross-linking properties (Fig. 3B). Two fragments, encoding aa 567-1327 and 629-1327, generated high molecular weight bands in the presence of cross-linker. Both novel bands are approximately double the predicted mass of the expressed fragments, suggesting dimerization rather than nonspecific cross-linking to reticulocyte lysate proteins. DHC fragments that only contain the central region produced some faint higher molecular weight smearing in the presence of DSP, but no distinct banding patterns could be detected. The fragments of the DHC amino terminus that do not contain the central region do not form high molecular weight species in the presence of cross-linker.

While the affinity resin and cross-linking assays do not generate identical results (summarized in Fig. 5), this may reflect subtle differences between the interactions being measured; the resin work measures the ability of a fragment to interact with the full 1327-aa amino-terminal polypeptide, whereas the cross-linking evaluates the interaction between two identical polypeptides. Nonetheless, the two assays agree that there is a crucial dimerization core region between aa 629 and 780, whose interaction is supported by flanking sequence on the carboxyl-terminal side. This additional sequence may play a role in stabilizing the structure of the interaction region.

Differential Interaction of DHC Subunits with DIC-- Because DHC amino terminus is also predicted to interact with the DIC, we constructed an affinity resin with bacterially expressed DIC and tested its ability to bind the DHC amino terminus. Reticulocyte-lysate-produced DHC also binds to DIC resin (Fig. 4A), with a 4-fold mean enhancement of binding over control. As in the DHC affinity resin, luciferase does not show an increased affinity for the DIC resin. This indicates that the DHC amino terminus also contains a binding site for the DIC.


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Fig. 4.   DHC fragment binding identifies a DIC binding site. A, lane 1 shows a Coomassie Blue-stained gel of the purified inclusion bodies containing the 60-kDa DIC fragment used to construct the DIC affinity resin. Lane 2 shows an immunoblot of the same sample probed with an intermediate chain antibody (23). B, the autoradiogram shows the relative binding of reticulocyte-expressed 1-1327-aa heavy chain fragment and luciferase for the DIC affinity resin versus a BSA control resin. Similar differences were obtained with actin or protein-free control resins. The graph below shows the quantified difference averages between the DIC and control resins for several independent binding experiments. C, the autoradiograms in the upper panel show representative pairs of several DHC fragments (encoded amino acids are listed below each pair; see Fig. 5 for a summary) bound to the DIC affinity resin and a control resin. The 1-1327 and luciferase samples are the same as shown in panel B. The lower panel provides the mean relative affinity of the DIC resin versus control resin from multiple independent experiments. The black bars represent fragments statistically considered to interact, the white bars represent noninteractors.

To search for a DIC interaction site within the DHC amino terminus, we tested the ability of the DHC fragment series to interact with DIC resin (Fig. 4B). The mapping pattern is similar to the DHC dimerization work but defines a smaller minimal region for this activity. The best statistical grouping of fragments predicted to contain the DIC-DHC interaction site is attained when fragments 1-1327, 567-1327, 629-1327, 1-730, 1-940, and 534-760 contain the site (P value = 0.012) (Fig. 5). This suggests that the DIC interaction site is located within aa 629-730, just upstream of the DHC-DHC site.


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Fig. 5.   Summary diagram of the amino-terminal DHC deletion series. The encoded DHC amino acids of each fragment are listed on the left. The relative positions of the fragments are shown in the middle. The right side of the figure summarizes data from Figs. 1-4, indicating which of these fragments did (+) or did not (-) show evidence of chemical cross-linking (X-LINK), binding to DHC affinity resin (DHC), or DIC affinity resin (DIC).

Heavy Chain and Intermediate Chain Binding Activities-- Despite mapping to the same region of the DHC sequence, the DHC and the DIC binding activities are not identical. We analyzed the resin assay interaction under conditions of varying ionic strength. While the homodimerization of the 1327-aa DHC fragments was salt-sensitive, the DHC-DIC interaction was stable even in the presence of 2 M NaCl (Fig. 6). This suggests that the DHC-DHC and the DHC-DIC interact through different mechanisms. The in vitro DHC-DIC interaction is partially disrupted in the presence of 0.6 M KI (not shown), a result consistent with the dissociation of the intermediate chain from native chicken cytoplasmic dynein (9).


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Fig. 6.   DIC and DHC affinity resins bind expressed DHC fragment by different interactions. Bound affinity resins were washed with 4 volumes of PHEM buffer containing either 250 mM or 2 M NaCl. The upper panel shows the representative autoradiogram while the bottom panel provides a graphical quantitation of the number of bound counts. The interaction between the 1-1327 polypeptide and the DIC affinity resin was much more stable in high salt (2 M NaCl) than with the DHC affinity resin.

In Vivo Expression of DHC Amino-terminal Fragments-- Previous attempts to overexpress the 1327-aa amino-terminal region of DHC in Dictyostelium suggest that large amounts of this polypeptide are toxic (21). A reasonable interpretation is that the fragment interacts with native dynein to produce defective molecules that impact cell viability. Because our in vitro work suggests that the central amino-terminal region is crucial for dynein assembly, fragments containing only this region should disrupt dynein assembly in vivo and cause severe phenotypic effects. To test this hypothesis, we attempted to express DHC constructs comprising aa 1-567 (amino), 567-817 (central), and 780-1327 (carboxy) in Dictyostelium. The amino- and carboxy-fragment expression is shown in Fig. 7. In cells containing either of these two constructs, a single polypeptide of the predicted size is abundantly expressed and is readily immunoprecipitated by anti-c-Myc monoclonal antibody. Consistent with the in vitro work, none of the dominant dynein subunits (DHC, DIC, or light intermediate chains) appear to co-purify here (Fig. 7) or on a c-Myc immunoaffinity column (not shown). Moreover, cells expressing these fragments do not have obvious phenotypic defects and grow at rates similar to those of wild type cells.


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Fig. 7.   Expression of amino-terminal fragments in vivo. Dictyostelium expression constructs containing aa 1-567 and 780-1406 of the DHC amino terminus were epitope-tagged with c-Myc and expressed in vivo. The first two lanes show the Coomassie Blue-stained high speed supernatant (HSS) and the c-Myc immunoprecipitate (IP) from Dictyostelium cells expressing the predicted 64-kDa polypeptide (aa 1-567). The second pair of lanes show an immunoblot of the same samples probed with an antibody to the amino terminus of the DHC (27). The high molecular mass native heavy chain can be seen in the HSS, but it is not enriched in the IP, suggesting that it does not interact with this amino-terminal fragment in vivo. The expressed fragment is also labeled by the antibody. The third pair of lanes show an identical immunoblot probed with an antibody to the Dictyostelium dynein intermediate chain (23). This polypeptide can also be seen in the HSS but is not enriched by immunoprecipitation. The second set of three panels follows the same order but show cells expressing the predicted 61-kDa fragment encoded by aa 780-1406. Again, there is no indication here that this fragment interacts with either the DHC or the DIC in vivo.

In contrast, attempts to express the central region (aa 561-817) of the amino terminus have been unsuccessful. Despite several transformations, no viable clones have been identified that express detectable levels of the predicted 29 kDa fragment.

Conservation of Sequence Among Cytoplasmic Dyneins-- The results described above suggest that at least two activities important for dynein subunit assembly are localized to a relatively small region of the DHC amino terminus. Sequences from nine species were compared with the amino-terminal region of the DHC of Dictyostelium (Fig. 8A). Homologous regions were aligned and the degree of identity to the Dictyostelium sequence was determined for intervals of 100 aa. While the amino-terminal region is generally not as conserved as the rest of the polypeptide (1, 11, 27), there are three striking areas of homology evident among the ten cytoplasmic sequences. The most conserved lies between aa 600 and 800 in the Dictyostelium sequence and includes the sequence we experimentally identify as essential for heavy-chain dimerization and heavy chain-intermediate chain binding. This further suggests that the assembly activities localized within aa 629-780 in Dictyostelium DHC are conserved among the other cytoplasmic dynein heavy chains.


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Fig. 8.   Sequence of the central region of the DHC amino terminus is conserved among the cytoplasmic dyneins. A, sequences homologous to Dictyostelium aa 1-1327 were determined for cytoplasmic DHCs of nine other species: Aspergillus nidulans (GenBankTM accession number P45444), Paramecium tetraurelia (Q27171), Drosophila melanogaster (P37276), Rattus norvegicus (P38650), Nectria hematococca (P78716), Saccharomyces cerevisiae (P36022), Schizosaccharomyces pombe (O13290), Neurospora crassa (P45443), and Caenorhabditis elegans (Q19020) (see Ref. 39). Sequence alignment was facilitated with the PileUp utility of GCG, and identity was calculated over 100-aa intervals. Three peaks of conservation are evident, and the most strongly conserved (aa 600-800) contains the region of Dictyostelium DHC essential for both DHC and DIC interactions (shaded band). B, amino termini of axonemal dynein heavy chains (Chlamydomonas reinhardtii beta  (Q39565) and gamma  (Q39575), Anthocidaris crassispina beta  (P39057), P. tetraurelia beta  (Q94709) (39)) were compared using C. reinhardtii beta  DHC as the standard. Areas of high homology are evident, but they do not align with the conserved regions of the cytoplasmic DHC amino termini.

Sequences for the axonemal Chlamydomonas, sea urchin, and Paramecium beta  DHCs, and for Chlamydomonas gamma  DHC, were also analyzed (Fig. 8B). The amino terminus of the Chlamydomonas beta  DHC interacts with several other dynein subunits (22), and the homologous regions of the sea urchin and Paramecium beta  DHCs show regions of good conservation with the Chlamydomonas beta  sequence. However, the overall pattern of conservation within the beta  amino-terminal fragments is distinct from the cytoplasmic dyneins.

    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The organization of the cytoplasmic dynein tail and the specific interactions between its subunits at the amino acid level have not been well characterized. Electron microscopy has been useful in localizing some of the subunits (4, 5), and considerable progress has been made in investigating interactions of these subunits with other dynein-associated proteins (15, 18-20). Subunit organization of axonemal dyneins has been primarily studied through isolation of dynein gene mutations (reviewed in Ref. 2). These often produce organisms with nonlethal motility defects, which are amenable to biochemical and structural analyses. Similar analyses are becoming informative for cytoplasmic dyneins (21, 23, 32-35), mapping out the heavy chain domain structure and identifying the microtubule-binding motif. However, the physical organization of the cytoplasmic dynein tail has not been dissected in sufficient detail to identify heavy chain domains that mediate interactions with other subunits. Because the tail region links the motor to a cargo, understanding the interactions here is important in determining how dynein is targeted and regulated.

Previous work in vivo suggested that the amino-terminal third of the dynein heavy chain is responsible for interactions between the DHCs and with other dynein subunits (21, 22). We used both chemical cross-linking and in vitro affinity methods here to identify a region that promotes DHC dimerization. This supports an earlier report suggesting recombinant cytoplasmic DHCs directly form multimers, even in the absence of other subunits (31). We have further determined that the sequences crucial for this interaction lie within an approximately 150-aa region of the DHC. While this core region is necessary for dimerization, it seems to function most efficiently in the context of flanking sequences. The additional amino acids may be important in stabilizing the structure of this central domain. The sequence on the carboxyl-terminal side, which likely forms at least part of the stalk connecting the tail to the globular head, is especially effective in this role.

We also show that the central domain of the DHC amino terminus directly interacts with the dynein intermediate chain in vitro. The sequence does not appear to require an additional structural support to bind the DIC, suggesting that the DHC and DIC interactions are distinct and that their activities are not dependent on one another. The different biochemical properties of the two interactions lend strength to this hypothesis.

The in vitro work is supported by in vivo expression of DHC fragments. The amino- and carboxy-regions of the DHC amino terminus can be expressed at high levels in Dictyostelium; selective immunoprecipitation of these fragments shows no evidence that they bind the DHC or DIC. We cannot rule out the possibility that the fragments are misfolded and their activities do not reflect native behavior. However, previous expression of the amino-terminal region indicated that larger fragments (158 kDa) are able to dimerize with a native heavy chain, suggesting some degree of proper folding (21). Attempts to overexpress the entire amino terminus in Dictyostelium led to the idea that this fragment contains a functional motif that can perturb native dynein activity, resulting in cell death. This lethal activity appears to be preserved within the central 29 kDa of the amino terminus, within the region we indicate contains the dimerization and DIC binding sites. We do not know whether the toxic effect is because of perturbation of DIC binding, dissociation of the two heavy chains, or both.

Because most dyneins form multi-polypeptide assemblies through interactions in their tail, it seems reasonable to expect some degree of conservation within this region. While the amino-terminal tail region of the DHC is less conserved than the carboxyl-terminal head domain (1, 11, 12), it does contain small areas of strong conservation. The highest degree of sequence identity corresponds to the area we identify here to mediate DHC and DIC interactions. Among cytoplasmic heavy chains, this region is generally 40-65% identical to the Dictyostelium sequence. Even the most divergent yeast sequences show some degree of conservation in this region. In contrast, axonemal dynein heavy chains show very little sequence conservation with cytoplasmic dyneins in this region (1, 2, 36-38, and this study). Given their conserved structures seen by electron microscopy, this seems surprising. It is certainly possible that tertiary structure is preserved in this region and that similar interaction mechanisms occur. However, axonemal dyneins more often form heterodimers or heterotrimers of distinct DHC polypeptides, whereas cytoplasmic dyneins dimerize from identical heavy chains. While the amino-terminal portion of the DHC is important for assembly in both cytoplasmic and axonemal dyneins, the mechanisms of interaction may be distinct.

The current work suggests a functional division of the DHC amino terminus into three regions. The central core contains an interaction domain that assembles two heavy chains and binds the intermediate chain. Moreover, expression of this region in vivo appears to generate a tool to disrupt native dynein assembly and activity. Directed mutagenesis and in vivo expression should prove useful here in determining the exact location and mechanism of the DHC dimerization and DIC interaction sites, thereby providing information on the interaction dynamics of the cytoplasmic dynein tail.

    ACKNOWLEDGEMENTS

We thank Dr. Andrew Reilly of the Computational Molecular Biology and Statistics Core (Wadsworth Center) for the statistical analyses and Dr. Renjie Song of the Molecular Immunology Core (Wadsworth Center) for assistance with phosphoimaging. We also gratefully acknowledge the Molecular Genetics Core Facility for oligonucleotide synthesis and DNA sequencing.

    FOOTNOTES

* This work was supported in part by a Grant GM51532 from the National Institutes of Health (to M. P. K.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

parallel To whom correspondence should be addressed: Wadsworth Center, Empire State Plaza, P. O. Box 509, Albany, NY 12201-0509. Tel.: 518-486-1490; Fax: 518-474-7992; E-mail: Michael.Koonce{at}wadsworth.org.

    ABBREVIATIONS

The abbreviations used are: DHC, dynein heavy chain; DIC, dynein intermediate chain; aa, amino acid(s); Pipes, 1,4-piperazinediethanesulfonic acid; PAGE, polyacrylamide gel electrophoresis; HSS, high speed supernatant(s); BSA, bovine serum albumin; DSP, dithiobis(succinimidyl propionate); EDC, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide.

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
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