Disruption of phacA, an Aspergillus nidulans Gene Encoding a Novel Cytochrome P450 Monooxygenase Catalyzing Phenylacetate 2-Hydroxylation, Results in Penicillin Overproduction*

José Manuel Mingot, Miguel Angel PeñalvaDagger , and José Manuel Fernández-Cañón§

From the Departamento de Microbiología Molecular, Centro de Investigaciones Biológicas del Consejo Superior de Investigaciones Científicas, Velázquez 144, Madrid, 28006, Spain

    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Aspergillus nidulans utilizes phenylacetate as a carbon source via homogentisate, which is degraded to fumarate and acetoacetate. Mutational evidence strongly suggested that phenylacetate is converted to homogentisate through two sequential hydroxylating reactions in positions 2 and 5 of the aromatic ring. Using cDNA substraction techniques, we have characterized a gene, denoted phacA, whose transcription is strongly induced by phenylacetate and which putatively encodes a cytochrome P450 protein. A disrupted phacA strain does not grow on phenylacetate but grows on 2-hydroxy- or 2,5-dihydroxyphenylacetate. Microsomal extracts of the disrupted strain are deficient in the NADPH-dependent conversion of phenylacetate to 2-hydroxyphenylacetate. We conclude that PhacA catalyzes the ortho-hydroxylation of phenylacetate, the first step of A. nidulans phenylacetate catabolism. The involvement of a P450 enzyme in the ortho-hydroxylation of a monoaromatic compound has no precedent. In addition, PhacA shows substantial sequence divergence with known cytochromes P450 and defines a new family of these enzymes, suggesting that saprophytic fungi may represent a source of novel cytochromes P450.

Phenylacetate is a precursor for benzylpenicillin production. phacA disruption increases penicillin production 3-5-fold, indicating that catabolism competes with antibiotic biosynthesis for phenylacetate and strongly suggesting strategies for Penicillium chrysogenum strain improvement by reverse genetics.

    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Aerobic degradation of aromatic hydrocarbons by microbes involves the action of oxygenases (enzymes that incorporate one or two atoms from dioxygen into substrates) acting at two different levels in specific catabolic pathways (1, 2). First, oxygenase enzymes acting at the upstream segment of these pathways incorporate one (monooxygenases, aromatic ring hydroxylases) or two (aromatic ring dioxygenases) oxygen atoms into the aromatic substrate as hydroxyl groups, preparing the ring for a subsequent ring-opening step. In this second step, the dihydroxylated aromatic ring is opened by ring-cleavage dioxygenases.

Monooxygenases are a mechanistically diverse group of enzymes (1) including, for example, flavoproteins such as p-hydroxybenzoate hydroxylase (3), multicomponent enzymes such as Pseudomonas mendocina toluene 4-monoxygenase, in which one of the terminal hydroxylase polypeptides contains a binuclear iron cluster (4), or heme-containing cytochrome P450 systems. Monooxygenases of the cytochrome P450 superfamily (5, 6) are widely distributed among living organisms and catalyze a multiplicity of biosynthetic and catabolic reactions, usually with narrow substrate specificity, including the hydroxylation of a variety of lipophylic drugs.

In common with other saprophytic microbes, the genetically amenable, obligate aerobic fungus Aspergillus nidulans shows notable metabolic versatility. For example, it can use the aromatic hydrocarbon compound phenylacetate (PhAc)1 as sole carbon source. Despite the abundant information available on the catabolic pathways of other aromatic compounds, which have been extensively studied in bacteria, our understanding of PhAc degradation pathways is scarce. In Pseudomonas putida U, it is known that PhAc is degraded through phenylacetyl-CoA (7), although the ring cleavage steps remain uncharacterized. In A. nidulans, PhAc degradation proceeds through 2,5-dihydroxy-PhAc (homogentisate, see Fig. 1). The three structural genes mediating the conversion of homogentisate to Krebs cycle intermediates (i.e. the "lower" PhAc pathway) have been characterized (8-10,) but the steps leading to homogentisate have not yet been reported. We describe here mutational and molecular analysis showing that A. nidulans PhAc catabolism proceeds via homogentisate through two sequential hydroxylating steps, of which the first is the 2-hydroxylation of the ring catalyzed by a novel cytochrome P450. Targeted inactivation of this gene results in penicillin overproduction.

    EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Fungal Strains, Media, and Growth Conditions-- A nidulans strains carried markers in standard use (11). Standard media for A. nidulans (12) were used for strain maintenance, growth tests, and transformation. Complementation tests were carried out in constructed diploids.

A biA1 strain was the source of cDNA, and a biA1 methG1 argB2 strain was the recipient strain for phacA gene disruption. A biA1 methG1 strain was used as wild type control in experiments with the disrupted strains. Culture conditions inducing high levels of expression of the PhAc catabolic genes have been described (13) and were used to grow mycelia for protein extraction. PhAc and its monohydroxy and dihydroxy derivatives were used as sole carbon source at 10 mM (although homogentisate was occasionally used at 25-50 mM), and 10 mM ammonium chloride was used as sole nitrogen source.

Isolation of PhAc Nonutilizing Mutants-- 2-, 3-, and 4-fluorophenylacetate at a 5 mM concentration were shown to prevent growth of A. nidulans in the presence of a derepressing carbon source, indicating that their catabolism was toxic for the mold. However, some residual growth was observed when plates were incubated for more than 3 days at 37 °C. Sectors of markedly more vigorous mycelia frequently arose after prolonged incubation. Although the reason why catabolism of these PhAc derivatives results in toxicity is not clear, preliminary tests showed that mutations preventing the toxicity of 2- and 3-fluorophenylacetate also prevented the catabolism of PhAc. Therefore, conidiospores of a yA2 pantoB100 strain were plated to obtain isolated colonies, which were transferred to minimal medium with 0.05% lactose (w/v) as carbon source in the presence of 5 mM 2- or 3-fluorophenylacetate. Sectors with more vigorous growth were purified and tested for the utilization of different PhAc derivatives as sole carbon source. Two major classes were found, which were denoted class I and II (see "Results").

phacA Gene Disruption-- A pUC18-based plasmid denoted pPhacA::argB was constructed by standard techniques. This plasmid contains (starting from the lacZ promoter in pUC18) 0.94 kbp of the phacA upstream region sequentially followed by its genomic coding region up to codon 297, a genomic, 3.2-kbp fragment containing an argB+ allele, the genomic sequence of phacA corresponding to codons 393-518, and finally 1.2 kbp of the phacA 3'-downstream region. This insert was purified from the plasmid after digestion with EcoRI and used for transformation (14). Transformed (arginine-independent) clones in which the resident phacA gene had been replaced by the transforming fragment were identified by Southern analysis. The mutated allele would encode a protein truncated at residue 297 and therefore would lack the predicted region involved in heme binding.

Characterization of phacA cDNA and Genomic Clones-- phacA cDNA clones were obtained by differential screening of a cDNA library enriched in PhAc-induced transcripts, as described (8-10). Seven cDNA clones were obtained. The insert of one such clone was used to isolate genomic clones from a standard lambda EMBL4 library. Cross-hybridizing sequences were mapped to two contiguous BamHI fragments, 2.4 kb and 1.9 kb long (a BamHI site was shown to split the phacA open reading frame). Nucleotide sequencing and comparison of genomic and cDNA clones revealed the intron-exon organization of the gene and showed that all seven cDNA clones were incomplete at the N-terminal coding region, the longest of which (at the 5'-end) ended within predicted codon 6. cDNAs including the predicted initiation codon were obtained by direct polymerase chain reaction amplification of the cDNA library using an internal phacA primer and lambda gt10-specific primers followed by a second polymerase chain reaction reaction primed with the above internal oligonucleotide and a second oligonucleotide ending 1 nucleotide upstream of the ATG codon. The presence of the ATG codon in the resulting cDNA product (and the absence of introns between codons 1 and 6) was confirmed by sequencing.

Preparation of Microsomes and Enzyme Assays-- Mycelia from the isogenic Delta phacA and phacA strains were pregrown in glucose minimal medium, washed, transferred to 10 mM PhAc minimal medium, and incubated for an additional 4 h at 37 °C to induce transcription of genes for PhAc catabolism (13). Cells were collected by filtration, washed, resuspended in 100 mM potassium phosphate buffer, pH 7.0, and disrupted with a glass bead beater (Braun, 0.5-mm glass beads) at 4 °C. Crude extracts were clarified after centrifugation at 22,000 × g for 15 min. Microsomal pellets were recovered after centrifugation at 100,000 × g for 1 h and resuspended in 100 mM potassium phosphate buffer, pH 7.0. These extracts contained 1-4 mg/ml protein. Enzyme activities of the microsomal extract were determined using standard procedures (15, 16) with minor modifications. NADPH-cytochrome P450 reductase was assayed in 1-ml reactions at 25 °C with 0.1 M phosphate buffer, pH 7.0, following the NADPH-dependent reduction of cytochrome c (0.05 mM initial concentration; epsilon 550 = 21 mM-1 cm-1) or ferrycyanide (0.5 mM initial concentration; epsilon 420 = 1.02) by the decrease of absorbance at 550 nm and 420 nm, respectively.

PhAc 2-hydroxylase was assayed by measuring the formation of 2-hydroxy-PhAc in a reaction that required PhAc (added at 1 mM) and NADPH (also at 1 mM) in the presence of a microsomal fraction. 2-Hydroxy-PhAc was chemically determined using diazotized 4-nitroaniline essentially as described (17). Absorbance was read at 550 nm and converted to nmol of 2-hydroxy-PhAc using a reference plot. The range of linear response was 1-100 nmol of 2-hydroxy-PhAc. This method also detected 3-hydroxy-PhAc, and therefore, the identity of the reaction product as 2-hydroxy-PhAc was confirmed by direct HPLC analysis of the reaction mixtures (see "Results"). Proteins were precipitated in the presence of 5% (w/v) trichloroacetic acid, and 10 µl samples were injected into a Nucleosil 300-5 C18 column (250 × 4 mm) coupled to a 11 × 4-mm precursor column of this support, using as mobile phase (at 0.6 ml/min) a solution containing 50 mM monohydrogen potassium phosphate, 0.1 mM EDTA, 100 mM trifluoroacetic acid, and 8% (v/v) acetonitrile. Detection was at 220 nm.

Penicillin Production-- Cultures for penicillin production were inoculated with spores of the Delta phacA or the phacA+ strain in penicillin production broth (18) with 2% lactose as the main carbon source, 2.5% (w/v) corn steep liquor, and the indicated concentrations of sodium phenylacetate. Flasks were shaken at 250 rpm at 37 °C. Samples were taken at different time points and used to measure penicillin using a bioassay with Micrococcus luteus, with penicillin G as standard (18).

    RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The "Upper" Phenylacetate Degradation Pathway-- We found that 2-, 3-, or 4-fluoro-PhAc prevents A. nidulans growth on 0.05% (w/v) lactose as carbon source. We therefore selected mutations resulting in fluorophenylacetate resistance, assuming that they would prevent PhAc utilization and following Apirion (19), who used fluoroacetate resistance to select acetate nonutilizing mutants. Mutations preventing PhAc utilization (phac) were efficiently selected with 2-fluorophenylacetate. They were recessive in diploids, indicating that they represent loss-of-function mutations. They were classified in two major classes. Class I mutants did not grow on PhAc but grew on 2-hydroxy-PhAc or 2,5-dihydroxy-PhAc. By contrast, class II mutants did not grow on either PhAc or 2-hydroxy-PhAc but grew on 2,5-dihydroxy-PhAc. Class I or II mutations did not affect growth on acetate, Phe, Tyr, 3- or 4-hydroxy-PhAc, and 3,4-dihydroxy-PhAc, showing that they specifically prevented PhAc catabolism. Mutations in class I complemented class II mutations. As 2,5-dihydroxy-PhAc is known to be an intermediate of PhAc catabolism (8-10), these data are consistent with the pathway shown in Fig. 1 in which PhAc is converted into homogentisate through two hydroxylating reactions, prevented by class I and class II mutations, respectively.


View larger version (33K):
[in this window]
[in a new window]
 
Fig. 1.   The A. nidulans phenylacetate degradation pathway. Shown are the steps required for PhAc degradation to Krebs cycle intermediates. Enzymes in the upper pathway are specific for PhAc. Enzymes in the lower pathway (gene names italized) are common to PhAc and Phe/Tyr catabolism (indicated with horizontal arrows). Phe and Tyr are also degraded through homogentisate.

Molecular Cloning of phacA, a Gene Encoding a Novel Cytochrome P450-- We have previously used a differential screening procedure of a subtracted cDNA library to isolate cDNA clones representing transcripts induced by PhAc (8-10). This collection of cDNAs included clones for the three genes (fahA, maiA, and hmgA) of the lower PhAc pathway (Fig. 1; Refs. 8-10). Among the remaining cDNA clones, those that did not represent these previously described genes were identified and classified by restriction enzyme mapping and/or partial cDNA sequencing. Seven overlapping cDNAs represented a novel PhAc-induced transcript whose gene was named phacA and which contained an open reading frame putatively encoding a 518-residue polypeptide (Mr 58,495). DNA sequencing of genomic clones showed that the phacA-coding region is interrupted by three introns, 65, 56, and 53 nucleotides long. The nucleotide sequence of phacA and the amino acid sequence of its derived protein product have been deposited in the DDBJ/EMBL/GenBank data bases under accession number AJ132442. Blastp searches against nonredundant Swissprot+Translation of EMBL nucleotide sequence data bases revealed that all 20 entries showing the highest amino acid sequence identity to PhacA were cytochrome P450 proteins of the CYP1 family (of which 19 were CYP1A1 P450s), including mammalian and fish proteins but only a single human CYP. Identity levels were in the 25% range, with the highest identity (27.4% in a 465 residue overlap) shown by a Sparus aurata (gilthead sea bream) P450 protein. Cytochromes P450 are heme-thiolate enzymes, and PhacA residues 431 to 439 contain the peptide motif Gly-Xaa-Gly-Xaa-Xaa-Xaa-Cys-Xaa-Gly (where Xaa indicates any amino acid), which is involved in heme binding in proteins of this class (6). We conclude that phacA encodes a cytochrome P450. The above levels of identity are below those required to place it in an already existing family (>40% identity required (5)). Therefore, PhacA defines a new family of these proteins.2 It has been denoted CYP504 using the current P450 nomenclature system.

phacA Is Strongly Induced by Phenylacetate-- phacA transcript levels were analyzed by Northern analysis in cells grown in glucose and subsequently transferred to minimal media, each containing a different carbon source. This analysis (Fig. 2) showed that phacA transcription is strongly induced by PhAc and largely repressed by glucose (PhAc plus glucose, Fig. 2). The transcript was absent in cells transferred to glucose alone or to gluconeogenic substrates acetate or glutamate. 2-Hydroxy-PhAc was also a strong inducer (although less so than PhAc), and 3-hydroxy-PhAc was a weak inducer. 4-Hydroxy-PhAc, 2,5-, or 3,4-dihydroxy-PhAc did not induce phacA transcription. Notably, Phe (but not Tyr) induced phacA transcription to some extent. These results support the contention that phacA is involved in PhAc catabolism.


View larger version (56K):
[in this window]
[in a new window]
 
Fig. 2.   Northern analysis of phacA transcript levels. Cells were grown on minimal medium with 0.3% (w/v) glucose as sole carbon source for 16 h at 37 °C and transferred to media with the indicated carbon sources (glucose at 1% (w/v), all aromatic compounds, and glutamate at 10 mM and potassium acetate at 30 mM; -carbon indicates no carbon source added). These secondary cultures were incubated for a further 1 h at 37 °C. Mycelia were then harvested and used to isolate RNA (18). The probe was a 1.2 kb phacA cDNA clone (4FG4). Actin transcript was used as loading control.

The Phenotype of a Disruption Strain Is Consistent with phacA Encoding a PhAc 2-Hydroxylase-- To confirm the involvement of PhacA in PhAc catabolism, we constructed a disruption-deletion phacA mutation by reverse genetics. We transformed an argB2 arginine-requiring strain with a linear DNA fragment carrying a mutated phacA gene in which a 3.2-kbp fragment containing an argB+ allele replaced a 289-base pair NaeI-KpnI phacA genomic fragment including codons 298-392 (see Fig. 3). This mutant phacA gene would encode a PhacA protein truncated at residue 297 and therefore lacking the 221 C-terminal residues, which include the essential Cys-containing peptide motif involved in heme binding. Homokaryotic transformant clones were purified after repeated streaking on medium lacking arginine and analyzed by Southern blot hybridization. Two transformants showing an identical hybridization pattern consistent with the integration event shown in Fig. 3 were chosen for further analyses. Both grew normally on 0.05% (w/v) lactose but, in contrast to the wild type, showed residual growth (similar to that observed in the absence of a carbon source) on PhAc-minimal medium (Fig. 3), showing that PhacA is indeed involved in PhAc catabolism. By contrast, both transformants were able to grow on 2-hydroxy-PhAc (Fig. 3). These data indicate that PhacA is involved in the ortho-hydroxylation of PhAc. The disruption-deletion mutation (denoted Delta phacA) was recessive in diploids (in agreement with its predicted loss-of-function phenotype) and did not complement phac-4, a prototype of classical class I mutations that, in common with Delta phacA, leads to inability to use PhAc but allows growth on 2-hydroxy-PhAc. phac-4 is therefore a phacA allele that we renamed phacA4.


View larger version (70K):
[in this window]
[in a new window]
 
Fig. 3.   Disruption of phacA. The phacA gene was replaced by a mutant version after transformation with a linear DNA fragment in which phacA codons 298-392 had been replaced by a 3.2-kbp DNA fragment containing the argB+ gene (see "Experimental Procedures"). This mutant allele encodes a PhacA protein truncated after residue 297.Two transformants (denoted Delta phacA #3 and #4) carrying the expected disruption-deletion mutation were purified and tested for growth on minimal medium with 0.05% (w/v) lactose, 10 mM PhAc, or 10 mM 2-hydroxy-PhAc as sole carbon source, as indicated. A wild type strain and a strain carrying a null (Delta hmgA) mutation in the homogentisate dioxygenase gene (see Fig. 1) were used as controls. Plates were incubated for 4 days at 37 °C before being photographed.

Delta phacA Microsomes Are Deficient in P450 PhAc 2-Hydroxylating Activity-- Most eukaryotic cytochromes P450 are microsomal enzymes. Electrons are transferred to their catalytic heme center from (NADPH)-cytochrome P450 reductase, a microsomal enzyme containing FAD and FMN (see Fig. 4A). We prepared microsomal fractions from wild type and Delta phacA cells induced with PhAc, which showed similar (NADPH)-cytochrome P450 reductase activity, as assayed by the NADPH-dependent reduction of artificial electron acceptors such as ferricyanide or cytochrome c (20) (Fig. 4A). The corresponding phacA+ and Delta phacA soluble fractions showed similar, high levels of homogentisate dioxygenase (a soluble enzyme of the PhAc degradation pathway, data not shown), indicating the equivalent induction of PhAc catabolism in both strains.


View larger version (22K):
[in this window]
[in a new window]
 
Fig. 4.   phacA disruption results in a marked reduction of microsomal PhAc 2-hydroxylase activity. A, a cytochrome P450 monooxygenase catalyzes the incorporation of one of the atoms from dioxygen (as an hydroxyl group) into the aromatic ring of PhAc. The source of electrons for such reaction is a NAPH-cytochrome P450 oxidoreductase, an enzyme that transfers electrons from NADPH through two flavin redox centers (20). In vitro, the activity of the reductase can be monitored by using artificial electron acceptors such as ferricyanide and cytochrome c, as indicated. Shown below are PhAc 2-hydroxylase and NAPH-cytochrome P450 oxidoreductase activities in the microsomal fractions of Delta phacA and phacA+ mycelia. Formation of 2-hydroxy-PhAc was monitored with a chemical method (17). B, HPLC analysis of PhAc 2-hydroxylase in the above microsomal fractions. The positions of standards were indicated by roman numbers as follows: I, 2,5-dihydroxy-PhAc; II, 3,4-dihydroxy-PhAc; III, 4-hydroxy-PhAc; IV, 3-hydroxy-PhAc; V, 2-hydroxy-PhAc; VI, PhAc. The retention time for authentic 2-hydroxy-PhAc was 11.82 min, whereas the product formed in the complete phacA+ and Delta phacA reactions showed retention times of 11.81 and 11.86 min, respectively.

Following incubation in vitro of wild type microsomes with PhAc and NADPH, we detected the formation of a monohydroxylated PhAc derivative using a chemical detection method ((Fig. 4A; see "Experimental Procedures"). Direct HPLC analysis of the reaction mixture showed that this compound was 2-hydroxy-PhAc and that its formation was absolutely dependent on the presence of both PhAc and NADPH (Fig. 4B). This showed that A. nidulans microsomes contain a phenylacetate ortho-hydroxylating activity. This activity was markedly and reproducibly reduced but not abolished by the Delta phacA mutation (Fig. 4, A and B). Finally, wild type mycelia pregrown in glucose and transferred to media containing PhAc secreted 2-hydroxy-PhAc to the culture supernatant (Fig. 5). In agreement with the above in vitro assays, secretion of 2-hydroxy-PhAc was also markedly reduced, but not abolished, by the Delta phacA mutation (Fig. 5). All these data, together with the growth characteristics of the Delta phacA strain (see above), strongly support the conclusion that phacA encodes a phenylacetate 2-hydroxylase and that a second, minor enzyme showing this activity is present in A. nidulans microsomes (see "Discussion").


View larger version (15K):
[in this window]
[in a new window]
 
Fig. 5.   Reduced secretion of 2-hydroxyphenylacetate in a Delta phacA strain. Culture supernatants of phacA+ (circles) and Delta phacA (triangles) strains were assayed for the presence of 2-hydroxy-PhAc. Mycelia were pregrown in glucose minimal medium and transferred to PhAc. Samples were taken at the indicated time-points after the transfer.

phacA Disruption Results in Increased Penicillin Production-- Penicillin-producing filamentous fungi use PhAc (activated as a CoA thioester) as a precursor for penicillin G biosynthesis. PhAc is exchanged with the L-aminoadipyl moiety of isopenicillin N to yield penicillin G in a reaction catalyzed by acyl-CoA::isopenicillin N-acyltransferase (see Ref. 21 for review). PhAc cannot be synthesized by fungi, and therefore it has to be fed to penicillin cultures. We reasoned that the PhAc degradation pathway would compete with the penicillin biosynthetic pathway for PhAc, suggesting that interrupting the initial step of the degradation pathway may improve the incorporation of PhAc into penicillins. This prediction was confirmed with the two above Delta phacA strains, which in independent experiments reproducibly showed a marked elevation in penicillin production over the wild type. Fig. 6 shows one such experiment, illustrating how the increase is already evident in cultures supplemented with 0.125% (w/v) PhAc (1.8 µg/ml in the wild type, 5.2 µg/ml in the Delta phacA strain). Moreover, reduction of PhAc to 0.0625% (w/v) resulted in nearly a 40% decrease in wild type penicillin production but had no decreasing effect in mutant strain production (Fig. 6; for simplicity, results with only one of the two disrupted strains are shown; see the legend). Under such conditions, the Delta phacA mutation resulted in a 4.9-fold increase in penicillin production.


View larger version (20K):
[in this window]
[in a new window]
 
Fig. 6.   Increased penicillin production resulting from phacA disruption. Time course of penicillin production in a Delta phacA (denoted here as phacA::argB) and a phacA+ strain. Media contained the indicated PhAc concentrations. PhAc is used as side-chain precursor for penicillin G biosynthesis (see text). Shown are data for transformant #3. Data for transformant #4 (not shown) were nearly indistinguishable in this and other penicillin production experiments.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

We have analyzed the "upper pathway" of PhAc catabolism (i.e. the conversion of PhAc to homogentisate, Fig. 1) in the filamentous fungus A. nidulans. Mutational and molecular evidence strongly indicates that this conversion proceeds via two sequential hydroxylating steps, the first of which is a 2-hydroxylation of the aromatic ring catalyzed by a cytochrome P450 monoxygenase encoded by the phacA gene. First, we have isolated a class of mutations preventing growth on PhAc but allowing growth on 2-hydroxy-PhAc or homogentisate as sole carbon source. Second, we describe a gene, denoted phacA, whose inactivation results in the same phenotype as the above mutations. This Delta phacA mutation does not complement with a prototypical classical mutation of the above class, indicating that both affect the same gene. Third, phacA encodes a CYP (cytochrome P450 monooxygenase). CYP enzymes are usually involved in a variety of biosynthetic and catabolic hydroxylating reactions (5, 6), in agreement with the predicted PhacA function. Fourth, phacA+ microsomal extracts catalyze the PhAc- and NADPH-dependent synthesis of 2-hydroxy-PhAc. By contrast, a mutant Delta phacA microsomal fraction is markedly deficient in this reaction. An A. niger microsomal, NADPH-dependent phenylacetate-2-monooxygenase activity previously reported by others (17) is possibly encoded by a phacA homologue. In agreement with our Northern analysis, such activity was detected in phenylacetate-grown cells and absent from glucose-grown cells (17).

The Delta phacA mutation constructed here is almost certainly a null mutation, which strongly suggested that the residual PhAc 2-hydroxylase activity that we detected with Delta phacA microsomal extracts is encoded by a different gene. We have identified the gene (denoted pshA) encoding this minor activity.3 In agreement with our prediction, a double Delta phacA Delta pshA mutation abolished microsomal PhAc 2-hydroxylation.3 A Delta pshA strain grows on PhAc but does not grow on 3-hydroxy-PhAc (which in the wild type is also catabolized through homogentisate), indicating that pshA encodes a 3-hydroxy-PhAc 6-hydroxylase (i.e. and ortho-hydroxylase), converting 3-hydroxy-PhAc to 2,5-dihydroxy-PhAc. This enzyme has been previously described in the fungus Trichosporum cutaneum, which converts PhAc to homogentisate through sequential hydroxylation of positions 3 and 6 of the ring (22).

Higher eukaryotes have multiple cytochrome P450 monooxygenases catalyzing a variety of oxidative reactions. Such abundance is not found in the microbial world. For example, only three CYP genes are found in the genome of S. cerevisiae (23). The marked metabolic versatility of filamentous fungi would suggest a greater variety of CYP enzymes in their proteomes. In addition to phacA, four stc genes of the A. nidulans sterigmatocystin biosynthetic cluster encode CYP enzymes (24). The closely related organism A. niger has a very specific benzoate-4 hydroxylase enzyme, encoded by the bphA gene (25). Notably, PhacA and BphA each define a new CYP family, suggesting that metabolically versatile saprophytic fungi may represent an as yet unexplored source of variability for CYP enzymes catalyzing novel metabolic reactions.

Industrial penicillin production by Penicillium chrysogenum strains requires the addition of PhAc, which is the side-chain precursor for the synthesis of penicillin G. Part of the added PhAc is oxidized (26), and it is not transformed into penicillin. Therefore, strain improvement programs using mutation and selection techniques have been directed to prevent such oxidation (27). Engineered expression in P. chrysogenum of a bacterial phenylacetyl-CoA ligase leading to increased levels of phenylacetyl-CoA available for penicillin biosynthesis resulted in penicillin overproduction (28). We show here that targeted disruption of the gene mediating the first step of A. nidulans PhAc catabolism results in a 3- to 5-fold increase in penicillin production and makes the recombinant strain less dependent on the external supply of PhAc. This presumably results from increased availability of phenylacetate for penicillin G biosynthesis. A P. chrysogenum phacA homologue has been identified. Therefore, these results pave the way for the improvement of P. chrysogenum industrial strains using a similar methodology.

    ACKNOWLEDGEMENTS

We thank E. Reoyo for technical assistance, Brian Nowak-Thompson, Eduardo Díez, and José Luis García for critical reading of the manuscript, Beatriz Galán and Auxi Prieto for their advice with HPLC analysis, and David R. Nelson for his assignment of an standarized cytochrome P450 designation to PhacA.

    FOOTNOTES

* This work was supported by Spanish Comisión Interministerial de Ciencia y Tecnología Grants BIO94-932 and BIO97-348 and Antibióticos S. A. U.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AJ132442.

Dagger To whom correspondence should be addressed. Fax: (34 91) 5627518; E-mail: cibp173{at}fresno.csic.es.

§ Present address: Dept. of Molecular and Medical Genetics, Oregon Health Sciences University, 3181 SW Sam Jackson Park Rd., L103 Portland, OR 97201-3098.

2 David R. Nelson, personal communication.

3 J. M. Mingot, M. A. Peñalva, and J. M. Fernández-Cañón, unpublished results.

    ABBREVIATIONS

The abbreviations used are: PhAc, phenylacetate; CYP, cytochrome P450; kbp, kilobase pair(s); HPLC, high performance liquid chromatography.

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
  1. Harayama, S., Kok, M., and Neidle, E. L. (1992) Annu. Rev. Microbiol. 46, 565-601[CrossRef][Medline] [Order article via Infotrieve]
  2. Harayama, S., and Timmis, K. N. (1992) in Metal Ions in Biological Systems (Sigel, H., and Sigel, A., eds), pp. 99-155, Marcel Dekker Inc., New York
  3. Schreuder, H. A., Prick, P. A., Wierenga, R. K., Vriend, G., Wilson, K. S., Hol, W. G., and Drenth, J. (1989) J. Mol. Biol. 208, 679-696[Medline] [Order article via Infotrieve]
  4. Whited, G. M., and Gibson, D. T. (1991) J. Bacteriol. 173, 3010-3016[Medline] [Order article via Infotrieve]
  5. Nelson, D. R., Koymans, L., Kamataki, T., Stegeman, J. J., Feyereisen, R., Waxman, D. J., Waterman, M. R., Gotoh, O., Coon, M. J., Estabrook, R. W., Gunsalus, I. C., and Nebert, D. W. (1996) Pharmacogenetics 6, 1-42[Medline] [Order article via Infotrieve]
  6. Nebert, D. W., and Gonzalez, F. J. (1987) Annu. Rev. Biochem. 56, 945-993[CrossRef][Medline] [Order article via Infotrieve]
  7. Olivera, E. R., Minambres, B., García, B., Muñiz, C., Moreno, M. A., Fernández, A., Díaz, E., García, J. L., and Luengo, J. M. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 6419-6424[Abstract/Free Full Text]
  8. Fernández-Cañón, J. M., and Peñalva, M. A. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 9132-9136[Abstract]
  9. Fernández-Cañón, J. M., and Peñalva, M. A. (1995) J. Biol. Chem. 270, 21199-21205[Abstract/Free Full Text]
  10. Fernández-Cañón, J. M., and Peñalva, M. A. (1998) J. Biol. Chem. 273, 329-337[Abstract/Free Full Text]
  11. Clutterbuck, A. J. (1993) in Genetic Maps. Locus Maps of Complex Genomes (O'Brien, S. J., ed), pp. 3.71-3.84, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
  12. Cove, D. J. (1966) Biochim. Biophys. Acta 113, 51-56[Medline] [Order article via Infotrieve]
  13. Fernández-Cañón, J. M., and Peñalva, M. A. (1997) Anal. Biochem. 245, 218-221[CrossRef][Medline] [Order article via Infotrieve]
  14. Tilburn, J., Scazzocchio, C., Taylor, G. G., Zabicky-Zissman, J. H., Lockington, R. A., and Davies, R. W. (1983) Gene 26, 205-211[CrossRef][Medline] [Order article via Infotrieve]
  15. Strobel, H. W., and Dignam, J. D. (1978) Methods Enzymol. 52, 89-96[Medline] [Order article via Infotrieve]
  16. Benveniste, I., Lesot, A., Hasenfratz, M. P., and Durst, F. (1989) Biochem. J. 259, 847-853[Medline] [Order article via Infotrieve]
  17. Sugumaran, M., and Vaidyanathan, C. S. (1979) FEMS Microbiol. Lett. 5, 427-430
  18. Espeso, E. A., and Peñalva, M. A. (1992) Mol. Microbiol. 6, 1457-1465[Medline] [Order article via Infotrieve]
  19. Apirion, D. (1965) Genet. Res. 6, 317-329[Medline] [Order article via Infotrieve]
  20. Vermilion, J. L., and Coon, M. J. (1978) J. Biol. Chem. 253, 8812-8819[Medline] [Order article via Infotrieve]
  21. Luengo, J. M., and Peñalva, M. A. (1994) in Aspergillus: 50 Years On (Martinelli, S. D., and Kinghorn, J. R., eds), pp. 603-638, Elsevier Science Publishers B.V., Amsterdam
  22. Anderson, J. J., and Dagley, S. (1980) J. Bacteriol. 141, 534-543[Medline] [Order article via Infotrieve]
  23. van den Brink, H. M., van Gorcom, R. F., van den Hondel, C. A., and Punt, P. J. (1998) Fungal Genet. Biol. 23, 1-17[CrossRef][Medline] [Order article via Infotrieve]
  24. Brown, D. W., Yu, J. H., Kelkar, H. S., Fernandes, M., Nesbitt, T. C., Keller, N. P., Adams, T. H., and Leonard, T. J. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 1418-1422[Abstract/Free Full Text]
  25. van Gorcom, R. F., Boschloo, J. G., Kuijvenhoven, A., Lange, J., van Vark, A. J., Bos, C. J., van Balken, J. A., Pouwels, P. H., and van den Hondel, C. A. (1990) Mol. Gen. Genet. 223, 192-197[Medline] [Order article via Infotrieve]
  26. Hockenhull, D. J. D., Walker, A. D., Wilkin, J. D., and Winder, F. G. (1951) Biochemistry 50, 605-609
  27. Lein, J. (1986) in Overproduction of Microbial Metabolites (Vanek, Z., and Hostalek, Z., eds), pp. 105-139, Butterworths, Stoneham, MA
  28. Minambres, B., Martínez Blanco, H., Olivera, E. R., Garcia, B., Diez, B., Barredo, J. L., Moreno, M. A., Schleissner, C., Salto, F., and Luengo, J. M. (1996) J. Biol. Chem. 271, 33531-33538[Abstract/Free Full Text]


Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.