O-Glycosylated MUC2 Monomer and Dimer from LS 174T Cells Are Water-soluble, whereas Larger MUC2 Species Formed Early during Biosynthesis Are Insoluble and Contain Nonreducible Intermolecular Bonds*

Magnus A. B. Axelsson, Noomi Asker, and Gunnar C. HanssonDagger

Department of Medical Biochemistry, University of Göteborg, Medicinaregatan 9, 413 90 Gothenburg, Sweden

    ABSTRACT
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

The MUC2 mucin is the major gel-forming mucin in the small and large intestine. Due to its sequence similarities with the von Willebrand factor, it has been suggested to dimerize in the endoplasmic reticulum and polymerize in the trans-Golgi network. Using an O-glycosylation-sensitive MUC2 antiserum, a dimerization has been shown to occur in the endoplasmic reticulum of LS 174T cells (Asker, N., Axelsson, M. A. B., Olofsson, S.-O., and Hansson, G. C. (1998) J. Biol. Chem. 273, 18857-18863). Using an antiserum immunoprecipitating O-glycosylated MUC2 mucin, monomers and dimers were shown to occur in soluble form in the lysate of LS 174T cells. The amount of O-glycosylated dimer was small, and no larger species were found even after long chase periods. However, most of the labeled MUC2 mucin was found in pelleted debris of the cell lysate. This insoluble MUC2 mucin was recovered by immunoprecipitation after reduction of disulfide bonds. Analysis by agarose gel electrophoresis revealed two bands, of which the smaller migrated as the O-glycosylated monomer and the larger migrated as the O-glycosylated dimer of the cell lysis supernatant. Mucins insoluble in 6 M guanidinium chloride could also be obtained from LS 174T cells. Such mucins have earlier been found in the small intestine (Carlstedt, I., Herrmann, A., Karlsson, H., Sheehan, J., Fransson, L.-Å., and Hansson, G. C. (1993) J. Biol. Chem. 268, 18771-18781). Reduction of the mucins followed by purification by isopycnic density gradient ultracentrifugation and analysis by agarose gel electrophoresis revealed two bands reacting with an anti-MUC2 tandem repeat antibody after deglycosylation. These bands migrated identically to the bands shown by metabolic labeling, and they could also be separated by rate zonal ultracentrifugation. These results suggest that the MUC2 mucin is forming nonreducible intermolecular bonds early in biosynthesis, but after initial O-glycosylation.

    INTRODUCTION
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Abstract
Introduction
Materials & Methods
Results
Discussion
References

The mucosal surfaces comprise a 1000-fold larger interface between the external and internal milieu than the skin. At the same time, the chemical and microbiological challenges are more demanding. The most important protective component of the mucosal surfaces is the mucus layer, the gel properties of which are due to macromolecules called mucins. A majority of the mucins known today belong to this classical gel-forming type, although a few glycoproteins defined as mucins are membrane-bound with yet unknown physiological functions (1). The present definition of mucin includes all glycoproteins that consist of more than 50% O-linked oligosaccharides and that have a majority of these oligosaccharides localized to mucin domains. These domains have a high number of O-glycosylated Ser and Thr amino acids, often appearing in tandem repeat sequences. Gel-forming mucins are probably altered in several diseases. Thus, alterations in the mucus barrier are probably essential in the pathogenesis of, for example, infections, peptic ulcers, and inflammatory bowel disease. Diseases such as cystic fibrosis and chronic bronchitis, and also trivial infections, are characterized by increased mucus viscosity. Despite its medical interest, the biochemical nature of these altered mucin properties is still poorly understood, largely due to the difficulties associated with the large size of these molecules.

The gel-forming mucins are proposed to be disulfide bond-stabilized linear polymers of highly glycosylated proteins (2), although other models are discussed (3). A typical example of such a mucin is encoded by the MUC2 gene, one of the few mucin genes fully sequenced (4). The MUC2 mucin occurs in small and large intestine (5, 6) and probably also in the airways upon epithelial stress, such as infection (7). The primary translation product, the mucin apoprotein, has a size of about 600 kDa, including N-glycans. It is composed of five major regions; three of these, one N-terminal, one C-terminal, and one central, are rich in Cys, whereas the two others are rich in Thr, Ser, and Pro. The two latter regions are the mucin domains, also called tandem repeat domains, and they become heavily O-glycosylated in the Golgi apparatus, thus obtaining extreme proteolytic resistance. The Cys rich N- and C-terminal regions show large similarities in the Cys positions to the corresponding parts of the von Willebrand factor (4, 8). This protein is known to form dimers stabilized by C-terminal disulfide bonds (9). The dimerization of the von Willebrand factor takes place in the endoplasmic reticulum, whereas a further N-terminal oligomerization occurs in the late Golgi compartments because it requires low pH (9). Due to the sequence similarities, a similar assembly procedure has been proposed for the MUC2 mucin. Using an antiserum (alpha -MUC2TR) directed against protein epitopes in the MUC2 tandem repeat, and thus only immunoprecipitating non-O-glycosylated MUC2 mucin species, we have previously shown that non-O-glycosylated MUC2 dimers are formed in LS 174T cells (10). Because O-glycosylation starts in the cis-Golgi or earlier, this indicates an endoplasmic reticulum-based dimerization in analogy with the von Willebrand factor. This assumption has now been verified by subcellular fractionation (11). By the help of an antiserum directed against protein epitopes unaffected by O-glycosylation, the monomer and dimer were identified in O-glycosylated form. However, the amount of especially O-glycosylated dimers was small, suggesting that these disappeared somewhere. The dimers were found to form insoluble MUC2 mucin occurring in cell debris pelleted from cell lysate. The oligomeric stage of this insoluble MUC2 mucin could not be analyzed, as reduction was necessary for solubilization. When analyzing the reduced insoluble MUC2 mucin, not only the monomeric forms expected from the von Willebrand factor analogy were found. Up to half the amount of monomers were linked together with nonreducible bonds and migrated as O-glycosylated dimers on agarose gels.

Mucins insoluble in chaotropic solutions, such as 6 M guanidinium chloride, have earlier been reported in the small intestine (12, 13), and in the gall bladder bile of some patients (14). Here we report that the cell line LS 174T can also form mucins insoluble in 6 M guanidinium chloride. These were found to contain the MUC2 mucin with nonreducible bonds, migrating similarly to the metabolically labeled insoluble MUC2 mucin from cell debris pellets.

    MATERIALS AND METHODS
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Antibodies and Cell Lines-- The polyclonal sera alpha -MUC2TR and alpha -MUC2N3 have been described (10, 11). An antiserum called alpha -MUC2C2 was raised in a rabbit against a synthetic peptide, CIIKRPDNQHVILKPGDFK, based on amino acids 4995-5013 on the C terminus of the human MUC2 apoprotein (4). A New Zealand White rabbit was immunized once with 500 µg of peptide conjugated via Cys to 400 µg of keyhole limpet hemocyanin in Freund's complete adjuvant, and twice with 250 µg of peptide conjugated to 200 µg of keyhole limpet hemocyanin in Freund's incomplete adjuvant. The interval between the immunizations was 2 weeks. The specificity of the alpha -MUC2C2 antiserum was tested as described for the alpha -MUC2N3 antiserum (11) using 1 mg of immunizing peptide/25 µl of antiserum for inhibiting immunoprecipitation. The colon adenocarcinoma cell line LS 174T (ATCC CL 188), producing MUC2 mucin, was cultivated as described before (10).

Pulse-Chase, Immunoprecipitation, and Electrophoresis Procedures-- Newly confluent LS 174T cells were starved for 1 h in 2 ml of methionine-free minimum essential medium (Life Technologies, Inc.) with 10% fetal bovine serum and 2 mM glutamine per 27-cm2 Petri dish and metabolically labeled using 150 µCi of [35S]methionine (Redivue Promix [35S] Cell Labeling Mix, Amersham Pharmacia Biotech) per Petri dish. Brefeldin A (Epicentre Technologies), 10 µg (solubilized in 5 µl of ethanol) per ml of medium, was added 15 min before metabolic labeling. Ammonium chloride (25 mM) was added at the start of starvation. In pulse-chase experiments, the cells were chased with culture medium, and when chase time exceeded 1 day, the medium was replaced daily. The cells were washed twice in cold PBS1 (137 mM NaCl, 2.7 mM KCl, 1.8 mM KH2PO4, 10.1 mM Na2HPO4) before lysis on ice by the help of a cell scraper in 1 ml (per Petri dish) of PBS with 50 mM Tris-HCl, pH 7.4, 5 mM EDTA, 1% Triton X-100, 5 mM N-ethylmaleimide, 0.5 mM phenylmethylsulfonyl fluoride (Sigma), 20 µg/ml aprotinin (Trasylol, Bayer), 60 µg/ml leupeptin (Sigma), 3.8 µg/ml calpain inhibitor I (Boehringer Mannheim), 0.7 µg/ml pepstatin (Boehringer Mannheim), 0.02% sodium azide. The lysate was sonicated three times for 2 s each (intensity 15, MSE Soniprep 100) and clarified by centrifugation. The cell debris pellet thus obtained was washed several times in PBS, and its mucin content was solubilized by reduction in 200 µl of PBS, 100 mM Tris-HCl, pH 8, 50 mM dithiothreitol (Merck) for 1 h at 37 °C under agitation. Material not solubilized by the reduction was pelleted by centrifugation and discarded, and the supernatant was incubated with 150 mM iodoacetamide (Sigma) for 30 min at room temperature under agitation in the dark, and then diluted with PBS to 1 ml. This solution and the clarified cell lysate were then handled in similar ways. They were incubated with 25 µl of preimmune rabbit serum for 30 min followed by 30 min with 300 µl of 10% (v/v) immunoprecipitin (Life Technologies, Inc.). After pelleting the immunoprecipitin by centrifugation, the supernatant was incubated under agitation for 2 h at room temperature or overnight at 4 °C with 50 µl of alpha -MUC2N3, 50 µl of alpha -MUC2C2, or 25 µl of alpha -MUC2TR. 300 µl of immunoprecipitin was then added before further incubation for 30 min. The immunoprecipitates were pelleted by centrifugation and washed four times in washing solution (10 mM Tris-HCl, pH 7.4, 2 mM EDTA, 0.1% Triton X-100, 0.1% SDS). The samples were released from the immunoprecipitin for 5 min at 95 °C in 50 mM Tris-HCl, pH 6.8, 20% glycerol, 5% SDS and, if reducing conditions denoted, also 5% beta -mercaptoethanol and 10 mM dithiothreitol. The immunoprecipitin was pelleted, and bromphenol blue (0.015%) was added to the supernatant prior to electrophoresis. Agarose gel electrophoresis (1.5 mm thick) was run on a vertical gel apparatus (140 × 160-mm gels, Hoefer) casted on agarose gel support medium (Gel Bond Film, FMC). The separation gel was 1% Ultrapure (Life Technologies, Inc.) and 1% Sea Plaque low gelling temperature (FMC) or 2% Ultrapure (Life Technologies, Inc.). The buffer contained 0.378 M Tris-HCl, pH 8.8, and 0.1% SDS. The stacking gel was made of 0.8% agarose (SeaKem Gold, FMC) in 0.126 M Tris-HCl, pH 6.8, and 0.1% SDS. The electrode buffer at the cathode was 0.05 M Tris, 0.384 M glycine, and 0.1% SDS, and at the anode 0.025 M Tris, 0.192 M glycine, and 0.05% SDS. The current for electrophoresis was about 10 mA. Gels were fixed for 3 h in 30% ethanol and 10% acetic acid, soaked in Amplify fluorographic reagent (Amersham Pharmacia Biotech) with 5% glycerol for 30 min, dried on slab gel dryer for 2 h at 50 °C, and exposed at -80 °C to a Biomax MS film (Kodak).

Purification of Insoluble Mucins Followed by Western Blot-- LS 174T cells were cultured in roller bottles for 10 days with daily medium changes and washed twice in cold PBS. The purification was performed as described before (13). In short, the attached cells were extracted with guanidinium chloride (6 M guanidinium chloride, 5 mM EDTA, 10 mM NaH2PO4, pH 6.5, 5 mM N-ethylmaleimide, and 0.5 mM phenylmethylsulfonyl fluoride), homogenized in a Dounce homogenizer (loose pestle), and incubated for 1 h at room temperature under agitation. Insoluble material was pelleted by centrifugation for 25 min at 40,000 × g, and the pellet was washed and centrifuged six times in similar guanidinium chloride buffer. The pellets were brought into solution by reduction of disulfide bonds in reduction guanidinium chloride (6 M guanidinium chloride, 100 mM Tris, 5 mM EDTA, 10 mM freshly added dithiothreitol, pH 8.0) for 5 h at 37 °C under gentle agitation. Cysteine groups were alkylated by the addition of 150 mM iodoacetamide (powder) followed by incubation overnight at room temperature under agitation in the dark. The mucins were then purified by three rounds of isopycnic density gradient ultracentrifugation (15), two with 4 M guanidinium chloride and one with 0.2 M guanidinium chloride. After unloading the gradient into fractions, the mucin peaks were identified by periodic acid-Schiff slot blot, dialyzed against water, lyophilized, redissolved, and boiled for 5 min at 95 °C in 50 mM Tris-HCl, pH 6.8, 20% glycerol, and 5% SDS. After electrophoresis performed as above (without Gel Bond Film and without stacking gel), the proteins were electrophoretically transferred (1 mA/cm2 for 3 h at +4 °C, Sartoblot II-S) to a nylon blotting membrane (Immobilon-P, Millipore) using 48 mM Tris, 39 mM glycine, 0.0375% SDS, and 10% methanol (pH 8.3) as transfer buffer. The membrane was washed several times in water after blotting and dried before deglycosylation with gaseous hydrogen fluoride at room temperature overnight (16). The membrane was then blocked in saturated casein (Sigma) in 150 mM NaCl, 20 mM Tris-HCl, pH 7.5, 0.1% Tween 20 for 1 h at room temperature and stained with 1% alpha -MUC2TR antiserum for 1 h at room temperature, followed by incubation for 1 h at room temperature with peroxidase-conjugated anti-rabbit antibody (DAKO) diluted 1:1000. Both antibody incubations were in 10% of saturated casein in 150 mM NaCl, 20 mM Tris-HCl, pH 7.5, 0.01% Tween 20. The assay was developed by the ECL reagent (Amersham Pharmacia Biotech), according to the manufacturers recommendations and using Biomax MS film (Kodak).

Rate Zonal Ultracentrifugation-- MUC2 mucin insoluble in 6 M guanidinium chloride was purified as described above, lyophilized, and redissolved in water (2 µg/µl). 20 µl of this solution was layered on top of a linear 22-43% (w/w) sucrose gradient in 150 mM NaCl, 20 mM Tris-HCl, pH 7.5, 0.02% NaN3, with a volume of 5 ml in a 13 × 51-mm ultracentrifuge tube (Beckman). The ultracentrifugation was performed in a Beckman swinging bucket rotor (SW55Ti) for 15 h at 40,000 rpm at +5 °C. Fractions (200 µl) were recovered from the top, and the water was evaporated in a vacuum centrifuge (Heto, Allerød, Denmark). The material was dissolved and boiled in 50 mM Tris-HCl, pH 6.8, 20% glycerol, 5% SDS and subjected to SDS-agarose gel electrophoresis, Western blotting, hydrogen fluoride treatment, and assay as described above. The intensity of the bands obtained on the film was measured by video densitometry using a video camera from KAPPA Messtechnik (Gleichen, Germany) and software from Bildanalys (Stockholm, Sweden).

Subcellular Fractionation-- Subcellular fractionation was performed as described elsewhere (11). An alternative homogenization technique was also used. Cells were resuspended in ice-cold 130 mM KCl, 25 mM Tris-HCl, pH 7.5, and protease inhibitors (11) and homogenized by passing through syringe needles (5 × 22 G/0.7 mm, 5 × 25 G/0.5 mm, 3 × 27 G/0.4 mm).

    RESULTS
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Migration of Non-O-glycosylated and O-Glycosylated MUC2 Species on SDS-Agarose Gel Electrophoresis-- We have previously shown that the alpha -MUC2TR antiserum precipitates only non-O-glycosylated MUC2 species (10), whereas the alpha -MUC2N3 antiserum also precipitates species migrating slower on SDS-agarose gels. These were regarded as O-glycosylated also because they occurred in the Golgi apparatus but not in the endoplasmic reticulum, of LS 174T cells (11). In order to further relate these different bands on SDS-agarose gel electrophoresis to each other, partially O-glycosylated MUC2 molecules, with intermediate sizes between non-O-glycosylated and O-glycosylated species, were obtained by Brefeldin A treatment of the LS174T cells and analyzed by SDS-agarose gel electrophoresis. An additional antiserum, supposed to immunoprecipitate both non-O-glycosylated and O-glycosylated MUC2, called alpha -MUC2C2, was also used to further verify that the different bands were indeed the MUC2 mucin.

Lysates from metabolically labeled LS174T cells were subjected to immunoprecipitation with the three different antisera mentioned, and the precipitates were analyzed by SDS-agarose gel electrophoresis and autoradiography (Fig. 1). Radioactive labeling for 20 min (Fig. 1A) revealed no O-glycosylated species, but the non-O-glycosylated monomer (M) was found by the O-glycosylation-sensitive antiserum directed against a MUC2 tandem repeat peptide (alpha -MUC2TR) (10) and by the alpha -MUC2N3 and the alpha -MUC2C2 antisera. The alpha -MUC2TR antiserum, reacting strongly compared with the two other sera, revealed that some non-O-glycosylated dimers (Fig. 1, D), also, had been assembled. When the cells were cultured for 3 h after radioactive labeling (Fig. 1C), O-glycosylated monomers (O-M) and dimers (O-D) were found by both the alpha -MUC2N3 and the alpha -MUC2C2 antisera. The O-glycosylation-sensitive alpha - MUC2TR antiserum, however, did not precipitate these species, but only the remaining non-O-glycosylated monomer and dimer. The O-glycosylated monomer migrated to a position slightly above the non-O-glycosylated dimer on the gel, whereas the O-glycosylated dimer migrated only a short distance from the stacking gel-separation gel interface. No larger bands specifically reacting with the alpha -MUC2N3 and alpha -MUC2C2 antisera could be found in the separation or stacking gels. Cells were also labeled for 30 min in the presence of Brefeldin A (Fig. 1B). This drug blocks the traffic of vesicles from the endoplasmic reticulum to the Golgi apparatus but not the traffic in the opposite direction, resulting in a transport of Golgi stack enzymes into the endoplasmic reticulum (17). As a result of this, glycosyltransferases from the proximal parts of the Golgi occur in the endoplasmic reticulum O-glycosylating proteins prematurely. Such O-glycosylation, however, is incomplete, giving smaller sized mucins than the native O-glycosylated molecule. Monomers (Fig. 1, o-M) and dimers (o-D) glycosylated in this way were precipitated by all of the antisera. This included the alpha -MUC2TR antiserum, reacting with a sequence repeated about 100 times in MUC2, whereas the Brefeldin A treatment probably gave a glycosylation of only some of the tandem repeats, allowing the antiserum to react with remaining non-O-glycosylated repeats. The partially O-glycosylated bands (Fig. 1, o-M and o-D), but not the non-O-glycosylated mono- and dimer bands (M and D), reacted with the Helix pomatia lectin (not shown). The monomers and dimers observed after Brefeldin A treatment migrated to a position between the non-O-glycosylated and the native O-glycosylated species. This shows that O-glycans decrease the electrophoretic mobility of the MUC2 mucin on SDS-agarose electrophoresis dependent on the amount of glycosylation and that migration on agarose gels could be used for interpreting the glycosylation status of mucins.


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Fig. 1.   Comparison of the mobility of non-O-glycosylated, partially O-glycosylated, and native O-glycosylated MUC2 species on SDS-agarose gel electrophoresis. Fluororadiography of SDS-agarose gel electrophoresis on immunoprecipitations from LS 174T cells labeled for 20 min (panel A), Brefeldin A treated and labeled for 30 min (panel B), and not treated, labeled for 2 h, and chased for 1 h (panel C). After cell lysis, cell debris discarding, and precipitation with preimmune serum, immunoprecipitations were performed with alpha -MUC2N3, alpha -MUC2C2, or alpha -MUC2TR. The separation gel was 1% Ultrapure (Life Technologies, Inc.) and 1% Sea Plaque low gelling temperature (FMC); the gel was analyzed under nonreducing conditions. The designation M refers to non-O-glycosylated monomer, D to non-O-glycosylated dimer, o-M to partially O-glycosylated monomer obtained by Brefeldin A treatment, o-D to partially O-glycosylated dimer obtained by Brefeldin A treatment, O-M to O-glycosylated monomer, and O-D to O-glycosylated dimer.

Larger MUC2 Species than O-Glycosylated Dimer Are Insoluble and Contain Nonreducible Intermolecular Bonds-- No MUC2 species larger than O-glycosylated dimer were observed on the agarose gels irrespective of the labeling and chase times used. To find out where the predicted larger forms of MUC2 were lost, the cell debris pelleted from the metabolically labeled cells, lysed with detergent and ultrasonicated, was reduced by dithiothreitol. The obtained solubilized material was immunoprecipitated with the alpha -MUC2N3 antiserum, followed by agarose gel electrophoresis (Fig. 2C). Two major MUC2 bands were found, of which the larger showed a partial separation into two. As a comparison, nonreduced MUC2 immunoprecipitated in the normal way from the clarified cell lysate is shown (Fig. 2B). This contains O-glycosylated monomer (O-M) and O-glycosylated dimer (O-D). A corresponding reduced lane was also included to demonstrate that the dimer (O-D) is reducible disappearing upon thiol reduction (Fig. 2A). The monomer (O-M) and dimer (O-D) correspond in size to the two main bands of the reduced insoluble portion. It seems therefore reasonable to believe that the upper band from the insoluble portion (Fig. 2C, O-X) consists of two monomers held together with bonds that are nonreducible by mercaptoethanol and dithiothreitol. This nonreducible material (O-X) is different from the in vivo-occurring reducible dimer (O-D) and will therefore be referred to as X-dimer in this paper. Bands migrating to the same positions as monomer and X-dimer could also be precipitated from the debris pellets by the alpha -MUC2C2 antiserum or by the H. pomatia lectin (not shown). No material at all in the reduced debris pellets could be precipitated using the alpha -MUC2TR antiserum (not shown). This shows that non-O-glycosylated MUC2 species are soluble and not incorporated into insoluble material. It should also be noted that no signs of nonreducible bonds have ever been observed in non-O-glycosylated MUC2 species (10, 11).


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Fig. 2.   Comparison of nonreduced and reduced soluble and reduced insoluble MUC2 mucin on SDS-agarose gel electrophoresis. Fluororadiography of SDS-agarose gel electrophoresis on immunoprecipitations with alpha -MUC2N3 of LS 174T cell lysate (panel A, reduced; panel B, nonreduced) and of the reduced corresponding cell debris pellet (panel C). Cells were labeled for 2 h, lysed, and ultrasonicated. Insoluble material was pelleted by centrifugation. The pellet obtained was washed in PBS, and the mucin content was solubilized by reduction in 50 mM dithiothreitol and alkylated in 150 mM iodoacetamide. Both the cell lysate and the reduced pellet were then subjected to preimmune immunoprecipitation followed by specific immunoprecipitation with alpha -MUC2N3. Shown is also a alpha -MUC2TR-stained Western blot of SDS-agarose gel electrophoresis on mucins insoluble in 6 M guanidinium chloride obtained from LS 174T cells (panel D). These mucins were solubilized by reduction in 10 mM dithiothreitol and alkylated in 30 mM iodoacetamide, prior to purification by three rounds of isopycnic CsCl density gradient ultracentrifugation, in which the fractions were assayed by periodic acid-Schiff slot blot. The result from the third centrifugation in 0.5 M guanidinium chloride is shown in panel E. Marked are the fractions pooled and subjected to dialysis, lyophilization, and SDS-agarose gel electrophoresis followed by Western blot. After deglycosylation, the Western blot was assayed using alpha -MUC2TR, peroxidase-conjugated secondary antibody, and the ECL reagent (panel D). For D, the same sample as in C was included, and it migrated identically on the gel (not shown). All gels used were 2% Ultrapure (Life Technologies, Inc.). The designation O-M refers to O-glycosylated monomer, O-D to O-glycosylated dimer (in panels A and B), and O-X to pairs of O-glycosylated monomers linked by nonreducible bonds, i.e. X-dimers (in panels C and D).

MUC2 Mucin, Which Was Insoluble in 6 M Guanidinium Chloride, Also Contained Nonreducible Bonds-- Mucins insoluble in 6 M guanidinium chloride have earlier been reported from small intestine (12, 13), where the major part in the rat has been shown to be due to Muc2 (6, 8). Cultivated LS 174T cells were extracted with 6 M guanidinium chloride, and the insoluble mucin complexes were pelleted by centrifugation and washed six times. The material was solubilized by dithiothreitol reduction and purified by three rounds of isopycnic density gradient ultracentrifugation in guanidinium chloride (15). The periodic acid-Schiff slot blot intensity of the fractions from the third preparative ultracentrifugation step, in 0.5 M guanidinium chloride, is shown in Fig. 2E, having a peak within the density interval between 1.53 and 1.49 g/ml, as expected for mucins (15). The material from these fractions was pooled and analyzed by SDS-agarose gel electrophoresis to compare the electrophoresis pattern to that obtained by immunoprecipitation. The mucins were Western blotted onto nylon membranes and deglycosylated by gaseous hydrogen fluoride before staining with the tandem repeat antiserum alpha -MUC2TR. Two bands were found, migrating to the same positions on the gel as monomer and X-dimer, respectively (Fig. 2D). No staining at all was found with alpha -MUC2TR on a non-deglycosylated control membrane (not shown). Because this antiserum does not bind O-glycosylated MUC2, this finding is further evidence that the species were O-glycosylated. This procedure also confirms that the bands at monomer and X-dimer position on the gels are indeed the MUC2 mucin, because the deglycosylation exposed the epitopes detected by the alpha -MUC2TR antiserum.

Separation of Monomer and X-dimer by Rate Zonal Ultracentrifugation-- As an alternative molecular size separation method to SDS-agarose gel electrophoresis, not dependent on electrical charge, the monomer and X-dimer were also subjected to rate zonal ultracentrifugation. MUC2 mucin insoluble in 6 M guanidinium chloride solubilized by reduction was layered on top of a sucrose gradient, which, after the centrifugation, was recovered as 25 fractions run on agarose gel and assayed by Western blot (Fig. 3A). The intensity of the obtained monomer and X-dimer bands in every fraction was measured by video densitometry (Fig. 3B). As expected for heterogeneously glycosylated molecules, both monomer and X-dimer showed a broad distribution over the gradient. Some of the material migrated to the bottom of the ultracentrifuge tube, probably due to aggregation, but not by covalent linkages because it could be dissolved by boiling in SDS prior to electrophoresis. Such aggregation might also have contributed to the broad distribution over the gradient. Both species, however, showed fairly distinct intensity maxima, probably representing similar glycosylation stages of nonaggregated molecules. These maxima were localized at 29.25% sucrose for the monomer and 31.5% sucrose for the X-dimer, giving sedimentation values of 14 S and 19 S, respectively, as calculated by the method of McEwen (18). As shown in Fig. 2E, both species had the same density, about 1.51 g/ml, as expected for O-glycosylated mucins (15). If the diffusion constant of the O-glycosylated MUC2 monomer is similar to that of monomer units from human cervical mucins in water (19), 4.7 × 10-8 cm2/s, our results would give a mass of the MUC2 monomer of about 2.7 × 106. The diffusion constant of the X-dimer is not known, making a calculation of its absolute mass impossible. However, together with the SDS-agarose gel electrophoresis results, the rate zonal ultracentrifugation makes it likely that the X-dimer indeed is a dimer. It also shows that the separation observed on the agarose gels is due to size differences and not, for example, charge.


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Fig. 3.   Separation of monomer and X-dimer by rate zonal ultracentrifugation. Western blot, performed as in Fig. 2D, showing every second fraction from a sucrose density gradient ultracentrifugation of the reduced and alkylated 6 M guanidinium chloride insoluble mucins from LS 174T cells (panel A). Also shown (panel B) are video densitometry measurements of monomer (bullet ) and X-dimer (open circle ) of all fractions from the ultracentrifugation, which were recovered from the top. Sucrose concentrations (black-square) according to refractive index measurements are shown. O-X and O-M are as in Fig. 2.

Pulse-Chase Analysis of the Appearance of Soluble and Insoluble MUC2 Mucin-- In order to study the appearance of non-O-glycosylated and O-glycosylated soluble MUC2 species, immunoprecipitation with the alpha -MUC2N3 antiserum (Fig. 4A) serially followed by immunoprecipitation with the alpha -MUC2TR antiserum (Fig. 4B) was performed on the soluble parts of the lysates from LS 174T cells that had been pulse-chased for up to 3 days. The O-glycosylated molecules appeared after about 1 h and reached maximum radiolabeling after about 2 h (Fig. 4A). The amounts of both species then quickly decreased to levels that still remained constant in the cells after three days. As shown previously (10, 11), the non-O-glycosylated precursors appeared within 0.5 h and showed maximum intensity after 1 h, as is also shown in Fig. 4B. The non-O-glycosylated monomer decreased to low levels after only a few hours, whereas the dimer, formed from the monomer, was still present in reasonable amounts after 24 h.


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Fig. 4.   Pulse-chase analysis of the appearance of soluble MUC2 mucin. Immunoprecipitations with the alpha -MUC2N3 antiserum (panel A), serially followed by immunoprecipitations with the alpha -MUC2TR (panel B), both on nonreduced SDS-agarose gel electrophoresis. LS147T cells were labeled with [35S]methionine for the time denoted under the lanes, or, when this exceeded 2 h, labeled for 2 h and chased with new medium for the rest of the time. The cells were then lysed, insoluble cell debris were pelleted, and precipitation with preimmune serum was performed on the supernatants, followed by specific precipitation. The separation gel used in panel A was 2% Ultrapure (Life Technologies, Inc.) and in panel B was 1% Ultrapure (Life Technologies, Inc.) and 1% Sea Plaque low gelling temperature (FMC). The gels were assayed by fluororadiography. O-D, O-M, D, and M are as in Fig. 1.

The appearance of the insoluble MUC2 mucin was studied by immunoprecipitation with the alpha -MUC2N3 antiserum on reduced debris pellets from the immunoprecipitations in Fig. 4. Both the monomers and X-dimers turned up after 1 h (Fig. 5), i.e. at the same time as the O-glycosylated bands in solution (Fig. 4A). The appearance of these insoluble forms of MUC2 this early suggests a fast synthesis of the nonreducible bonds and a cellular location relatively early in the biosynthesis pathway. The bands remained in the cells in the same intensity range after 3 days. In other experiments, high activity was still observed after 5 days, and weaker activity was observed after 7 days (not shown). The lowered radiolabeling intensity at 3 and 8 h is, to our experience, not real but rather a randomly occurring artifact, probably caused by incomplete reduction (incomplete solubilization) or destruction of the antibodies by the reducing or alkylating agents.


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Fig. 5.   Pulse-chase analysis of the appearance of insoluble MUC2 mucin. Fluororadiography of SDS-agarose gel electrophoresis on immunoprecipitations with alpha -MUC2N3 on the reduced cell debris pellets from the experiment in Fig. 4 (panel A) and from LS 174T cells metabolically labeled for 2 h and chased for 24 h in the presence of 25 mM ammonium chloride (panel B). The pellets were washed in PBS, the mucin content solubilized by reduction in 50 mM dithiothreitol and alkylated in 150 mM iodoacetamide prior to preimmune immunoprecipitation followed by specific immunoprecipitation with alpha -MUC2N3. The gel was 2% Ultrapure (Life Technologies, Inc.). O-X and O-M are as in Fig. 2.

Subcellular Fractionation-- In an attempt to localize the cellular location for the formation of the insoluble MUC2 molecules, subcellular fractionation was performed, using two different types of homogenization. However, all of the insoluble material after homogenization was recovered in the pellet obtained by centrifugation at 1,400 × g prior to subcellular fractionation (not shown). No nonreducible X-dimers were found in the subcellular fractions. This means that the appearance of insoluble MUC2 could not be studied by subcellular fractionation.

Nonreducible Bond Formation Does Not Require Low pH-- The N terminus of the MUC2 mucin shows similarities in the localizations of the Cys residues with the von Willebrand factor. Because the von Willebrand factor dimers are known to form polymers stabilized by N-terminal disulfide bonds by a process that requires an acidic pH (9), a similar mechanism has been proposed for the MUC2 mucin. No disulfide-stabilized MUC2 species larger than dimers were detected in this study. To study whether a lowered pH could influence the formation of the nonreducible bonds in MUC2, LS 174T cells were cultured in the presence of ammonium chloride. This neutralizes the acidic pH in the distal Golgi apparatus and secretory vesicles (20). The ratio between monomer and X-dimer was constant after ammonia treatment (Fig. 5B), although the amount of both species was decreased, which we have noticed before.2 This indicates that the formation of the nonreducible bonds in MUC2 is not pH-sensitive.

    DISCUSSION
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

The striking similarities in the localizations of the Cys residues between the N and C termini of MUC2 and the von Willebrand factor made it likely that the assembly followed similar pathways. The present results show that this is not the case, and instead an insoluble form of MUC2 is formed early via intermolecular bonding that cannot be reduced. The present results are dependent on the specificity of the anti-MUC2 antisera available. The antisera alpha -MUC2N3 and alpha -MUC2C2 were able to precipitate glycosylated MUC2 monomers and reducible dimers from the soluble cell lysate portion and monomers and nonreducible X-dimers from reduced insoluble cell debris pellet. The conclusion that the immunoprecipitated species were O-glycosylated was based on the observations that they (i) were larger than the non-O-glycosylated species and the incompletely glycosylated species produced upon Brefeldin A treatment (Fig. 1), (ii) appeared later in time during pulse-chase than the non-O-glycosylated species (Fig. 4A), (iii) showed nonreducible size polydispersity on the gels, most probably due to heterogeneous glycosylation, (iv) were localized to the Golgi apparatus according to subcellular fractionation (11), (v) reacted with the H. pomatia lectin (11), and (vi) were not immunoprecipitated by alpha -MUC2TR (Fig. 1). In addition, monomers and X-dimers from the guanidinium chloride insoluble preparation were regarded as O-glycosylated because they (vii) reacted with the alpha -MUC2TR antiserum on Western blot only after deglycosylation (Fig. 2D), (viii) had a density of 1,51 g/ml (Fig. 2E), as expected for O-glycosylated mucins (15), and (ix) had higher sedimentation coefficients on ultracentrifugation than (SDS-treated) non-O-glycosylated species, as for monomers 14 S (Fig. 3B) and 8 S (11), respectively. It should be noted that the alpha -MUC2TR antiserum is much more efficient in immunoprecipitating MUC2 than the O-glycosylation-insensitive antisera, as shown in Fig. 1. This makes immediate kinetic comparisons between the amounts of O-glycosylated and non-O-glycosylated MUC2 mucin, immunoprecipitated with the different types of antisera, impossible. The reason for the difference in effectiveness is not totally clear, but it might include different antibody titers and a large consumption of O-glycosylation-insensitive antibodies by epitopes on nonlabeled MUC2 mucin stored in the cells. The O-glycosylation-insensitive antisera are directed against nonrepeated protein epitopes, whereas the epitopes of alpha -MUC2TR are tandemly repeated about 100-fold in every molecule. This might also contribute to the differences wherein the MUC2TR antiserum should be less sensitive for the peptide conformation as caused by, for example, the high number of probable Cys-linkages in the nontandem repeat regions. Because the MUC2 apomucin is very large, repeated binding sites might also minimize mechanical dissociation from the immunoprecipitin during the washing steps.

The two bands observed on the agarose gels of reduced insoluble MUC2 mucin were regarded as a monomer and a dimer (called X-dimer), because they migrated similarly on the gel to the monomer and reducible dimer of the soluble MUC2 mucin. Because mobility comparisons are uncertain when dealing with broad bands caused by heterogeneous O-glycosylation, and because separation could be due also to charge, rate zonal ultracentrifugation was performed to verify that the monomer and X-dimer were indeed of different size. This analysis suggested a mass of 2.7 MDa for the monomer using a previously published diffusion coefficient for human cervical mucins (19). With a predicted size for the non-O-glycosylated MUC2 monomer of 600 kDa, this suggests that the mass is made up of about 80% O-glycans, which is within the usual range of mucin glycosylation (13, 21). The lack of information on the diffusion constant for the X-dimer makes it impossible to calculate its mass and state that it is indeed a dimer. For human cervical mucins, which have MUC5AC, MUC5B, MUC2, and MUC6 as known gel-forming components (22, 23), it has been shown that oligomers, with the average size of four monomer units, have a sedimentation coefficient about twice that of the monomers (40.4 for oligomers and 19.2 S for monomers in water) (19, 21). The sedimentation coefficient of the X-dimer was only 1.3-1.4 times larger than that of monomer, and if oligomerization does not affect the diffusion properties of MUC2 and human cervical mucins in very different ways, these figures may suggest that the X-dimer consists of two or, less likely, three monomer units. The ratio between sedimentation coefficients for non-O-glycosylated, SDS-treated MUC2 dimer and monomer was just above 1.2, as was the ratio between apoB100 and apoB53/48 (11). Dimerization of O-glycosylated and non-O-glycosylated MUC2 apomucins may affect the diffusion constants in proportional ways, if O-glycans and bound SDS, respectively, cause similar stretching of the tandem repeat regions. If this is the case, one should thus expect identical ratios. The small differences found for the ratios, together with the results from the SDS agarose gel electrophoresis support the interpretation that the X-dimer is indeed a dimer.

The finding of nonreducible bonds associated with MUC2 mucin insolubility was an unexpected phenomenon. Because the insoluble MUC2 portion had to be solubilized by reduction prior to analysis, the rate of disulfide bond-stabilized oligomerization could not be investigated. It is therefore impossible to say whether nonreducible links between disulfide bond-stabilized dimers are enough to give the insolubility or whether the mucins are further oligomerized by disulfide bonds before the nonreducible links are assembled. If the von Willebrand factor analogy is correct, no further oligomerization than dimerization should occur until the trans-Golgi network, because such oligomerization requires low pH. It seems possible that the nonreducible bonds are assembled earlier because their formation does not require low pH, as shown by ammonium chloride treatment (Fig. 5B). As already mentioned, no nonreducible bonds could be seen using alpha -MUC2TR either in supernatant (10, 11) or pellet (not shown), showing that no non-O-glycosylated species were linked with such bonds. This indicates a localization of the nonreducible bond formation in the Golgi rather than in the endoplasmic reticulum. Attempts to verify this theory by two different subcellular fractionation protocols failed, as the insoluble MUC2 mucin was found already in the cell debris routinely pelleted after homogenization, and thus was excluded from the ultracentrifugation step. It is not clear why the insoluble mucins were pelleted in these experiments when they should have been encapsulated into membrane vesicles. One possibility for this could be that they gave the whole vesicle a density high enough for pelleting; another possibility is that they in some way caused the vesicles to rupture. However, vesicles containing Golgi markers were obtained at subcellular fractionation and contained soluble O-glycosylated mono- and dimers (11). This might suggest that there could exist different vesicles containing soluble and insoluble MUC2 mucins.

The nature of the nonreducible bonds is presently unknown. The fact that the linkages are nonreducible and survive 6 M guanidinium chloride or boiling in 5% SDS suggests an uncomplicated covalent nature. This linkage could be between amino acids within the primary MUC2 sequence. Another possibility is that the bonding is via smaller linking proteins or peptides. Mucins have previously been proposed to be polymerized via a linking peptide, but an isolated linking peptide has been shown to be encoded by the C terminus of Muc2 (24, 25).

The X-dimers, obtained by reduction, are obviously soluble because they can be immunoprecipitated. Our results contradict that such soluble particles exist in vivo in substantial amounts (Fig. 2A). Thus, nonreducible bonds between monomers seem to be absent or rare in vivo. If monomers are not forming nonreducible links, this suggests a conformational change of the MUC2 molecule upon disulfide-stabilized dimer formation, allowing the formation of the nonreducible bond. This would imply that the sites for the two different kinds of bonds are localized near each other on the molecule, in the C terminus if the von Willebrand factor analogy is correct. If the nonreducible bonds are C-terminal and if they are formed in the Golgi, one may speculate on the presence of a specific enzyme system catalyzing their formation. Attempts were made to move an eventual bond formation catalyzing enzyme system from the Golgi to the endoplasmic reticulum by the help of Brefeldin A. This did not, however, give rise to any nonreducible bonds after 30 min either in the soluble or insoluble portion of the cell lysate (not shown). If the formation of the nonreducible bonds takes place in the early Golgi, the C terminus heparin binding domain on the MUC2 mucin could participate in some way, because this domain is cleaved off already in the cis-Golgi apparatus (26). Such a function of this domain might be to bind heparan sulfate and thus orient the MUC2 dimers in a way facilitating nonreducible bond formation.

In Fig. 2B, the X-dimer band consists of two equally strong adjacent bands. The presence and intensity of the upper of these is, in our experience, highly variable, and often only one X-dimer band can be found. However, the intensity of this additional band had a tendency to increase upon prolonged pulse-chase times (Fig. 5A). The variable additional bands indicate that some MUC2 monomer residues might be involved in more than one nonreducible bond and that the formation of the second bond could be delayed. The presence of nonreducible bonds within MUC2 from the intestine has been proposed by Carlstedt et al. (27). They have shown that reduced MUC2 from intestinal tissue can form a ladder of bands upon agarose gel electrophoresis, suggesting that more than two nonreducible bonds can be formed in vivo.

Mucins that are insoluble in 6 M guanidinium chloride are consistently found in the small intestine (12, 13) and, to a more variable extent, in for example the gall bladder (14). The physiological function of mucin insolubility could be to improve the mucus barrier in water-filled lumen organs. The finding that both guanidinium chloride insoluble MUC2 mucins and the metabolically labeled water-insoluble MUC2 mucins contain the unexpected X-dimers suggests that water and guanidinium chloride insolubilities are reflecting an identical phenomenon. This could propose that one of the key events in the formation of the very resistant and insoluble MUC2 is the formation of nonreducible bonds. How the bonds give rise to insolubility is not clear. It could also be the case, of course, that they are not vital for insolubility but are only synchronized with disulfide bond-stabilized polymerization and/or oligosaccharide elongation, yielding the insolubility. An attractive hypothesis, however, could be that the nonreducible bonds serve as cross-links between linear MUC2 oligomers.

    FOOTNOTES

* This work was supported by Swedish Medical Research Council Grants 7461 and 10443, the IngaBritt and Arne Lundbergs Stiftelse, the Göteborg Medical Society, and the Swedish Cystic Fibrosis Foundation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed. Tel.: 46-31-7733488; Fax: 46-31-416108; E-mail: gunnar.hansson{at}medkem.gu.se.

1 The abbreviation used is: PBS, phosphate-buffered saline.

2 M. A. B. Axelsson, N. Asker, and G. C. Hansson, unpublished data.

    REFERENCES
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

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