COMMUNICATION
Protein Kinase B/Akt Induces Resumption of Meiosis in Xenopus Oocytes*

Carsten B. AndersenDagger §, Richard A. Roth, and Marco ContiDagger parallel

From the Dagger  Department of Gynecology and Obstetrics, Division of Reproductive Biology, and  Department of Molecular Pharmacology, Stanford University School of Medicine, Stanford, California 94305-5317

    ABSTRACT
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

The activation of protein kinase B/Akt is thought to be a critical step in the phosphoinositide 3-kinase pathway that regulates cell growth and differentiation. Because insulin-like growth factor 1 stimulates the resumption of meiosis in Xenopus laevis oocytes via phosphoinositide 3-kinase activation, we investigated the Akt involvement in this process. Injection of mRNA coding for a constitutively active Akt in Xenopus oocytes induced germinal vesicle breakdown (GVBD) to the same extent as progesterone or insulin treatment. Injection of mRNA coding for the wild type Akt kinase was less effective in stimulating GVBD, whereas Akt bearing a lysine mutation in the catalytic domain that abolishes the kinase activity had no effect. A mutant Akt lacking a membrane-targeting sequence did not induce GVBD, despite high levels of expression and activity. As previously reported for insulin, induction of GVBD by Akt was prevented by incubating the oocytes with cilostamide, an inhibitor specific for the type 3 phosphodiesterase (PDE3), suggesting that the activity of a PDE is required for Akt action. That an increase in PDE activity in the oocyte is sufficient to induce meiotic resumption was demonstrated by expression of an active PDE protein. In addition, the constitutively active Akt caused a 2-fold increase in the activity of the endogenous PDE. These data demonstrate that Akt is in the pathway controlling resumption of meiosis in the Xenopus oocyte and that regulation of the activity of a PDE3 is a step distal to the kinase activation.

    INTRODUCTION
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

A critical step in growth factor stimulation of the target cell is the activation of the phosphoinositide 3-kinase (PI3-K)1 pathway. This signaling pathway has been implicated in a wide array of cellular events including mitogenesis, transformation, differentiation, and regulation of metabolism (1). Recently Akt, also known as protein kinase B or related to the A and protein kinase C (2-4), was identified as a kinase distal to PI3-K (1). Three Akt isoforms (alpha , beta , and gamma ) with closely related properties have been identified. These are proteins of approximately 60 kDa containing a pleckstrin homology (PH) domain (5) and a serine/threonine kinase domain structurally related to the catalytic domains of protein kinases A and C. Phosphatidylinositol 3,4-bisphosphate and 3,4,5-trisphosphate, the products of PI3-K, bind to the PH domain of Akt and serve to anchor the enzyme to the membrane as well as to induce a conformational change in the enzyme (6). The subsequent phosphorylation of Akt, at Thr-308 in the catalytic domain and Ser-473 of the C terminus of Aktalpha , is crucial for the activation of Akt (7, 8). At least one of the kinases phosphorylating Akt has been identified as the 3-phosphoinositide-dependent kinase-1 (9-11).

Akt is activated by insulin and IGF-1 (12), and this regulation mediates the activation of glucose uptake (13), the phosphorylation and deactivation of GSK3 (14), and activation of p70 S6 kinases (15). Akt also phosphorylates the proapoptotic protein Bad, promoting its interaction with 14-3-3 and thereby preventing apoptosis (16).

In somatic cells including adipocytes, insulin regulates the activation of cGMP-inhibited phosphodiesterase (PDE3) (17). This activation is mediated by PI3-K because wortmannin, a PI3-K inhibitor, blocks the insulin-dependent activation of the PDE (18). The PDE3B isoform expressed in adipocytes is phosphorylated following insulin stimulation, and the phosphorylation is associated with an increase in PDE activity (19). This PDE activation and the consequent decrease in intracellular cAMP are probably responsible for the decrease in the hormone-sensitive lipase activity (17) and for the antilipolytic effects of insulin. The properties of the kinase phosphorylating PDE3B have not been firmly established, although a recent report indicates that Akt coelutes with a kinase activity that phosphorylates PDE3B in a cell-free system (20).

In Xenopus laevis oocytes, IGF-1 induces the dissolution of the nuclear membrane (GVBD) and completion of the first meiotic division (21). Although distinct signaling pathways may be activated, IGF-1, or the physiological stimulus progesterone, activates meiotic resumption by inducing a transient decrease in intraoocyte cAMP levels, thereby reducing the protein kinase A activity (22, 23). It has been proposed that activation of a PDE and a decrease in cAMP are crucial steps for reentry into the cell cycle. The effects of insulin and IGF-1 on meiosis are blocked by cilostamide, a specific inhibitor of the type 3 family of phosphodiesterases (24). Furthermore, insulin treatment or injection of an activated Ras leads to an increase in the PDE activity in the Xenopus oocyte (25).

Here we have tested the hypothesis that Akt is involved in the IGF-1-induced resumption of meiosis by activating a PDE present in the Xenopus oocyte. Our data demonstrate that Akt is a signal for resumption of meiosis in the Xenopus oocyte and that activation of a PDE3 is a step distal to the Akt activation.

    MATERIALS AND METHODS
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Abstract
Introduction
Materials & Methods
Results
Discussion
References

Construction of mRNA Expression Vectors-- The constructs encoding the wild type (WT)-Aktalpha , myristoylated (myr)-Akt, K179M-Akt, and A2myr-Akt have been described (12, 13). These constructs were transferred from the pECE vector to the XbaI-SalI sites of the mRNA expression vector pSP64(poly(A)) (Promega, Madison WI). The pCMV-5 iSH2-p110 (a gift from M. Birnbaum) was subcloned into pSP64(poly(A)) using XbaI and BamHI for the p110 subunit and XbaI for the inter-SH-2 domain (iSH2-I) of p85. The pCMV-5 rat PDE4D3 construct has been described previously (26). The complete open reading frame of PDE4D3 was subcloned from pCMV-5 into EcoRI of pBluescript KSII and then into pSP64(poly(A)) using AvaI-HindIII. All Akt constructs had the influenza virus HA epitope tag fused in-frame to the C terminus to monitor protein expression.

In Vitro mRNA Synthesis-- To express Akt, PI3-K, and PDE4D3 mRNAs, the pSP64(poly(A)) constructs were transcribed using the SP6 polymerase according to the procedure supplied by the manufacturer (Message Machine Kit, Promega). The transcribed mRNA was purified by phenol/chloroform extraction, precipitated at -20 °C with 1 volume of isopropyl alcohol, and resuspended in diethylpyrocarbonate water. The mRNA concentration was measured by A260, and transcript size was determined by formaldehyde gel electrophoresis. The mRNA was diluted to 1 mg/ml in diethylpyrocarbonate water and stored at -70 °C.

Injection into Xenopus Oocytes-- Ovary fragments were surgically removed from pregnant mare serum gonadotropin-primed X. laevis, and the oocytes were isolated manually. Stage VI oocytes were selected for all the experiments. Oocyte storage and experiments were carried out in OR2 solution (82.5 mM NaCl, 2.5 mM KCl, 1.0 mM CaCl2, 1.0 mM MgCl2, 1.0 mM Na2HPO4, 5.0 mM HEPES, pH 7.8). Oocytes were routinely tested for their insulin responsiveness by incubation of 10 oocytes in 1 µM insulin in OR2 solution overnight at room temperature. Oocytes that exhibited an insulin-stimulated GVBD of 80-100% were used. The mRNA or H2O (vehicle) was injected using a micromanipulator (Drummond) into defolliculated Xenopus oocytes. Resumption of meiosis was scored by the appearance of a white spot on the animal pole of the oocyte.

Western Blot Analysis-- Expression of HA-tagged Akt was analyzed after lysing injected oocytes in 10 µl of lysis buffer (250 mM sucrose, 1 mM KCl, 1 mM MgCl, 0.2 mM phenylmethylsulfonyl fluoride) per oocyte. Oocyte extracts were isolated by centrifugation at 15,000 × g for 10 min at 4 °C. The lipid supernatant was removed, and the clarified supernatants were transferred to Eppendorf tubes. Oocyte extract were analyzed by gel electrophoresis on 8% SDS-polyacrylamide gel electrophoresis (1:30 bis:acrylamide). After transfer, nitrocellulose membranes were blocked overnight at 4 °C in 5% bovine serum albumin in Tris-buffered saline with 0.02% Tween 20. Immunostaining to detect Akt-HA expression was performed by incubating for 1 h with a 1:1000 dilution of an HA.11 polyclonal antibody (Babco) and subsequently with a 1:5000 dilution of anti-rabbit Ig conjugated to horseradish peroxidase (Amersham Pharmacia Biotech). Blots were visualized by the ECL procedure (Amersham Pharmacia Biotech). Western blot for endogenous ERK2 phosphorylation was performed as described previously (27).

In Vivo PDE Assay-- PDE activity was measured in Xenopus oocytes by the method described by Sadler and Maller (23). In brief, oocytes were first stimulated with insulin or injected with either PI3-K, K179M-Akt, or myr-Akt mRNA. Three hours later, they were reinjected with 50 nl of 2 mM [3H]cAMP to a final concentration of 200 µM (500-700 cpm/pmol). The reaction products were separated and quantitated as previously described (23, 28).

Akt Assay-- Oocytes were lysed as described above, and the expressed Akt was immunoprecipitated and analyzed for activity as described (12). GSK peptide GRPRTSSFAEG was used as substrate (14). Akt activity was determined by both densitometry and scintillation counting.

    RESULTS
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Abstract
Introduction
Materials & Methods
Results
Discussion
References

Akt Stimulation of Meiotic Resumption in Xenopus Oocytes-- To test whether Akt is involved in the resumption of meiosis, an mRNA encoding a constitutively active Akt (7) was microinjected in Xenopus oocytes; in this construct, the Akt coding region with a deletion in the PH domain (Delta 4-129) is fused to a 14-amino acid leader containing the myr sequence of src (myr-Akt). This engineered protein is constitutively active in 3T3L1 cells by being targeted to the membrane (7). The expression of this constitutively active Akt caused resumption of meiosis as measured by GVBD (Fig. 1). The effect of active Akt expression was comparable with the insulin or progesterone stimulation (Fig. 1). When the time course of GVBD was monitored after myr-Akt injection, half-maximal maturation occurred 9.3 ± 1.6 h after injection (data not shown), whereas progesterone- and insulin-induced GVBD occurred with half-times of 2.9 ± 0.3 and 4.8 ± 0.7 h, respectively.


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Fig. 1.   Microinjection of mRNA coding for a constitutively active Akt (myr-Akt) stimulates meiotic resumption in Xenopus oocytes. Stage VI oocytes were treated with either 1 µM insulin or 10 µM progesterone (not injected groups) or injected with 50 nl of H2O or 50 ng (1 mg/ml) of myr-Akt (injected groups). The number in parentheses denotes the number of oocytes injected for each group. Oocytes were incubated at 20 °C after treatment, and GVBD was scored after 20 h. Maturation is expressed as the percentage of oocytes exhibiting GVBD ± S.E. of five independent experiments.

Additional constructs were used to determine whether the kinase activity and the membrane targeting are necessary for the Akt-induced resumption of meiosis. The glycine in position 2 of the myristoylation sequence of myr-Akt was mutated to alanine (A2myr-Akt). This possesses basal activity but is not constitutively activated, presumably because its interaction with the membrane is disrupted. A kinase-deficient mutant was obtained by a lysine to methionine mutation (K179M) in the ATP binding motif (K179M-Akt). Constructs encoding a full-length Akt clone (WT-Akt) and a constitutive active PI3-K were used as controls. Oocytes injected with myr-Akt produced 100% GVBD, whereas the WT-Akt was less effective (Fig. 2A). The myristoylation-deficient A2myr-Akt and kinase-deficient K179M-Akt were both inactive in promoting meiotic resumption (Fig. 2A). Western blot analyses using an anti-HA polyclonal antibody demonstrated that the A2myr-Akt and the K179M-Akt proteins accumulated at high levels in the oocyte (Fig. 2B). This finding rules out the possibility that the constructs with the point mutations are inactive because of instability and impaired accumulation of these mutant proteins. The assay of the Akt kinase activity confirmed that constitutively active myr-Akt has an 8-10-fold higher activity than WT-Akt, whereas the K179M-Akt has no measurable activity (Fig. 2C). Although completely inactive in the induction of meiosis, the A2myr-Akt had considerable kinase activity (Fig. 2C). These data indicate that Akt has to be both localized and active to stimulate resumption of meiosis. Western blot analyses of Xenopus extracts with mitogen-activated protein kinase antibodies verified that injection of the constitutively active Akt, but not the kinase-dead mutant, induces phosphorylation of ERK2 together with the induction of GVBD (data not shown).


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Fig. 2.   Stimulation of meiotic resumption by different Akt constructs. A, stage VI oocytes were subjected to the following injections or treatments: stimulated with 10 µM progesterone or 1 µM insulin (Not injected group); or injected with 50 nl of H2O or 50 ng of mRNA per oocyte encoding PI3-K, myr-Akt, A2myr-Akt, WT-Akt, or K179M-Akt (Injected group). Maturation is expressed as the percentage of oocytes exhibiting GVBD, and the values are the means ± S.E. of three independent experiments with a total of 60 or more oocytes per group. B, Western blot of the expressed HA-Akt. Immunostaining was performed using anti-HA.11. C, Akt activity of the expressed Akt. Akt activity was analyzed as described under "Materials and Methods." Results shown are representative of the two experiments performed.

Activation of a PDE3 Is Distal to Akt in the Maturation Pathway-- Previous work by Sadler (24) had shown that the maturation induced by insulin and IGF-1 is blocked by type 3 PDE inhibitors. To investigate whether a PDE is distal to Akt, different concentrations (0.2, 0.5, and 1.0 mg/ml) of constitutively active Akt (myr-Akt) mRNA were injected into Xenopus oocytes preincubated with inhibitors of PDE3. A 1-h preincubation with 10 µM cilostamide effectively prevented the myr-Akt-induced meiotic resumption (Fig. 3). Under the same conditions, 10 µM cilostamide also blocked insulin-induced maturation (data not shown). Whereas preincubation of oocytes in 10 µM cilostamide reduced the effect of microinjected myr-Akt no inhibition was observed by preincubating oocytes with a type 4 PDE inhibitor, rolipram (data not shown). This suggests that Akt-stimulated resumption of meiosis requires the activity of a PDE3, as does IGF-1-stimulated oocyte maturation (24).


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Fig. 3.   Effect of PDE3 inhibitors on the Akt-stimulated resumption of meiosis. Oocytes were injected with 50 nl of either 0.2, 0.5, or 1.0 mg/ml myr-Akt mRNA with or without preincubation for 1 h in 10 µM cilostamide (Cil), as indicated. Resumption of meiosis (GVBD) was scored at 15 h after injection, and the results are reported as the percentage of oocytes exhibiting GVBD. The mean ± S.E. of three independent experiments is reported.

To determine whether PDE activation per se is sufficient to induce resumption of meiosis, a PDE mRNA was injected into the oocytes. The mRNA coding for a PDE4 (PDE4D3) rather than PDE3 was used for these experiments to distinguish the expressed PDE from the endogenous PDE activity. Injection of the PDE4D3 mRNA induced GVBD to the same extent as progesterone or insulin treatment (Fig. 4). This effect was blocked by inhibitors specific for type 4 PDE (10 µM rolipram) but not by inhibitors specific for type 3 PDE (cilostamide) (Fig. 4). This finding demonstrates that the activity of the expressed PDE4 is required for induction of meiosis. It is also important to note that cilostamide could not block the meiotic resumption induced by the PDE4D3 injection, thus ruling out the possibility that the effect of this inhibitor on the Akt-stimulated maturation is because of effects other than PDE3 inhibition.


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Fig. 4.   Effect of PDE mRNA microinjection on meiotic resumption. Oocytes were treated with 10 µM progesterone with or without a 1-h preincubation in 10 µM rolipram (Not injected). Alternatively, they were injected (Injected) with 50 nl of H2O or 50 ng per oocyte rat PDE4D3 mRNA with or without 1-h preincubation with either 10 µM cilostamide (+cil.) or 10 µM rolipram (+rol.). Maturation is expressed as the percentage of oocytes exhibiting GVBD. Results are averages of two independent experiments on 40 or more oocytes per group.

To test whether Akt activates the endogenous PDE, we next measured cAMP hydrolysis. Oocytes were injected with 50 ng of myr-Akt mRNA 3 h prior to injection of [3H]cAMP (23). Oocytes injected with K179M-Akt were used as a control. In parallel, a group of oocytes was stimulated with insulin 2 h before [3H]cAMP injection. Injection of the constitutively active Akt caused a 2-fold increase in cAMP hydrolysis over the control (Table I). As previously reported (23), insulin treatment also stimulated cAMP hydrolysis (data not shown).

                              
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Table I
Akt stimulation of oocyte PDE activity in vivo
Stage VI oocytes were injected with 50 ng of mRNA encoding either constitutively active PI3-K, constitutively active (myr) Akt, or kinase-deficient (K179M) Akt. After 3 h the oocytes were reinjected with 50 nl of 2 mM [3H]cAMP. The cAMP hydrolysis was measured in three groups of five oocytes per sample as described under "Materials and Methods." Values are averages ± S.D.

    DISCUSSION
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Akt is a serine-threonine kinase in the PI3-K signaling pathway that mediates growth factor regulation of cell differentiation and survival (1). Here we provide evidence for an additional role of this Akt kinase in the control of the cell cycle and meiosis. Akt expression in the Xenopus oocyte causes germinal vesicle breakdown and mitogen-activated protein kinase phosphorylation, hallmarks of the resumption of meiosis. Furthermore, our findings indicate that a PDE3 is distal to Akt activation. This conclusion is supported by both the pharmacological manipulation of the oocyte PDE and by the observation that an increase in PDE activity follows expression of the Akt kinase in oocyte. Finally, the finding that expression of a PDE in the oocyte per se is sufficient to promote a meiotic resumption is consistent with the above conclusions.

Numerous observations have underscored the importance of the lipid PI3-K in insulin and growth factor signaling in somatic cells (1) as well as in Xenopus oocytes. Because insulin and IGF-1 effects on meiosis are inhibited by wortmannin (29), PI3-K has been implicated in oocyte maturation. Injection of a constitutively active PI3-K mRNA causes resumption of meiosis (30), whereas injection of the lipid phosphatase SIP/SHIP blocks the insulin effects (31). Our data extend these observations by identifying downstream steps in the pathway by which IGF-1 induces resumption of meiosis. As for somatic cells where the PI3-K effects are mediated by activation of Akt, we have shown that Akt activates resumption of meiosis in a manner similar to insulin/IGF-1.

Although the myr-Akt efficiently promoted oocyte maturation, a wild type Akt was only partially effective in the oocyte model, consistent with observations in mammalian cells showing that wild type Akt has low basal activity in the absence of insulin or growth factor signals (7, 8, 12, 13). Unlike the myr-Akt, the A2myr-Akt lacking the PH domain and with a mutation in the myristoylation signal was ineffective in inducing resumption of meiosis. This protein was efficiently expressed, and the overall Akt activity recovered in oocyte extracts was comparable with that obtained by expression of myr-Akt. When the activity of A2myr-Akt was corrected for the amount of protein expressed, this mutant kinase had one-fourth the specific activity of the myr-Akt. Because the only difference between A2myr-Akt and myr-Akt is the ability to interact with the lipid bilayer, we can conclude that Akt interaction with lipids is essential for the signaling meiotic resumption.

Our findings that PDE3 inhibitors block the oocyte maturation induced by insulin and Akt, but not progesterone, indicate that a PDE3 is involved in the PI3-K signal transduction pathway activated by IGF-1/insulin. In agreement with this hypothesis, we have shown that insulin, PI3-K, and Akt stimulate the activity of PDE3 endogenous to the oocyte. That Akt activation of PDE3 may be a sufficient signal for meiosis resumption is strongly suggested by the observation that expression of a PDE causes GVBD. Our conclusion is consistent with the finding in adipocytes, where it has been shown that insulin through the PI3-K pathway activates PDE3B (32).

Our data do not exclude the possibility that Akt is at a branch point in the signaling pathway controlling maturation. Upon activation, this kinase may phosphorylate several substrates in addition to a PDE3, and the activation of a PDE3 has only a permissive role on meiotic maturation. According to this model, both the release of a cAMP-dependent blockade and the activation of a distinct signaling cascade may be required for meiotic resumption. For instance, Akt activates the p70 ribosomal S6 kinase, which may be involved in the regulation of mos translation, a crucial step in oocyte meiosis (33).

Regardless of the mechanism of Akt action, our findings indicate a role for this enzyme in oocyte maturation. Interestingly, the cloning of 3-phosphoinositide-dependent kinase-1, the kinase-phosphorylating Thr-308 of Aktalpha , has uncovered a structural and function homology with the Drosophila DSTPK61 kinase. Although little is known about the function of the PI3-K and Akt pathway in Drosophila, DSTPK61 kinase may play an important role in the differentiation of the female and male germ cells (9). Our data in the Xenopus oocyte are in line with the view that this pathway is involved in oocyte maturation. The physiological signals activating the PI3-K and the Akt pathway are unknown at present but may be a means by which somatic cells control the function of germ cells. It is worth noting that the PDE3A mRNA (34) and protein are expressed in mammalian oocytes. Consistent with that shown in Xenopus, PDE3 inhibitors block the spontaneous resumption of meiosis in rat and mouse oocytes as well as the maturation induced by the luteinizing hormone in the intact follicle (34, 35). Recent in vivo observations have further confirmed the crucial role of PDE3 for resumption of meiosis in mammals (35). Thus, our data on the Xenopus oocyte open the possibility that, in the mammalian follicle, physiological signals promoting resumption of meiosis regulate the intraoocyte cAMP levels via the PI3-K/Akt signaling pathway.

    ACKNOWLEDGEMENTS

We thank Dr. Jim Ferrell for the polyclonal anti-ERK2 antibody and the helpful discussion; Eric Machleder and Mike Wu for help with microinjections; Morris Birnbaum for iSH2-p110 clone; and Kristina Kovacina and Aimee Kohn for pECE-Akt constructs.

    FOOTNOTES

* This work was supported in part by National Institutes of Health Grants HD20788 and P50 HD31398 Project IV (to M. C.) and DK34976 (to R. A. R.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Supported by Grant UDL9526 from The Danish Research Academy and Grant 9600503 from The Danish Research Councils.

parallel To whom correspondence should be addressed. Tel.: 650-725-2452; Fax: 650-725-7102; E-mail: marco.conti{at}forsythe.stanford.edu.

1 The abbreviations used are: PI3-K, phosphatidylinositol 3-kinase; PDE, phosphodiesterase; IGF-1, insulin-like growth factor 1; HA, hemagglutinin; myr, myristoylation; PH, pleckstrin homology; GVBD, germinal vesicle breakdown; WT, wild type.

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Abstract
Introduction
Materials & Methods
Results
Discussion
References

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