Identification of a Novel AMP-activated Protein Kinase beta  Subunit Isoform That Is Highly Expressed in Skeletal Muscle*

Claire Thornton, Michael A. SnowdenDagger , and David Carling§

From the Medical Research Council Clinical Sciences Centre, Cellular Stress Group, Imperial College School of Medicine, Hammersmith Hospital, DuCane Road, London W12 0NN and Dagger  Enzyme Pharmacology Unit, GlaxoWellcome Research Group, Stevenage, Hertfordshire SG1 2NY, United Kingdom

    ABSTRACT
Top
Abstract
Introduction
Procedures
Results
Discussion
References

The AMP-activated protein kinase (AMPK) is a member of a growing family of related kinases, including the SNF1 complex in yeast, which respond to nutritional stress. AMPK is a heterotrimeric complex of a catalytic subunit (alpha ) and two regulatory subunits (beta  and gamma ), and proteins related to all three subunits have been identified in the SNF1 complex. We have used the two-hybrid system in order to identify proteins interacting with the catalytic subunit (alpha 2). Using this approach, we have isolated a novel AMPKbeta isoform, which we designate AMPKbeta 2. The N-terminal region of beta 2 differs significantly from that of the previously characterized isoform (beta 1), suggesting that this region could play a role in isoform-specific AMPK activity. Comparison of the C-terminal sequences of beta 1 and beta 2 with their related proteins in yeast identifies two highly conserved regions predicted to be involved in binding of the alpha and gamma  subunits. The expression of beta 1 and beta 2 was examined in a number of tissues, revealing that the beta 1 isoform is highly expressed in liver with low expression in skeletal muscle, whereas the opposite pattern is observed for the beta 2 isoform. These results suggest that the beta  isoforms have tissue-specific roles, which may involve altered responses to upstream signaling and/or downstream targeting of the AMPK complex.

    INTRODUCTION
Top
Abstract
Introduction
Procedures
Results
Discussion
References

In mammals, the AMP-activated protein kinase (AMPK)1 plays a major role in the response to metabolic stress (1-3). AMP activates AMPK via a number of independent mechanisms, including activation of an upstream kinase (AMPKK), which in turn phosphorylates and activates AMPK (4, 5). The effects of AMP are antagonized by high concentrations of ATP so that it appears that the kinase responds to the AMP/ATP ratio, rather than AMP itself (4). Once activated, AMPK phosphorylates a number of enzymes involved in biosynthetic pathways causing their inactivation and preventing further ATP utilization. These findings have led to the hypothesis that the AMPK system has evolved to monitor the energy status, or fuel supply, within the cell (2-4).

Molecular characterization of AMPK has revealed that it is composed of three distinct subunits: a catalytic subunit, alpha  (molecular mass approximately 63 kDa); and two regulatory subunits, beta  (30 kDa) and gamma  (36 kDa) (6-10). In vitro binding studies indicate that the alpha  and beta  subunits and the beta  and gamma  subunits interact directly, whereas the alpha  and gamma  subunits do not form a stable interaction (10). The formation of the heterotrimeric complex may therefore be mediated, at least in part, by the beta  subunit. Proteins related to all three subunits have been identified in the SNF1 kinase complex in Saccharomyces cerevisiae, which is involved in the derepression of glucose-repressible genes (11). AMPKalpha is 47% identical to Snf1p (we refer to the individual subunits as Snf1p, etc., and the complex as SNF1) (6), AMPKgamma is 35% identical to Snf4p (10, 12), and AMPKbeta is related to the Sip1p/Sip2p/Gal83p family of proteins (10, 12). In addition to their primary sequence similarities, the AMPK and SNF1 complexes are functionally related since SNF1, like AMPK, phosphorylates and inactivates acetyl-CoA carboxylase (13). Indeed, the similarities between the two complexes extend even further as it has recently been shown that active SNF1, e.g. under glucose derepressing conditions, most likely exists as a heterotrimeric complex of Snf1p, Snf4p, and one of the Sip1p/Sip2p/Gal83p proteins (14, 15). Furthermore, analogous to AMPK, formation of the complex is mediated by one of the Sip1p/Sip2p/Gal83p family of proteins (14, 15).

A second alpha  isoform has been isolated recently, which was termed, somewhat unconventionally, alpha 1 (the isoform that was first identified becoming alpha 2) (16). It was reported that the alpha 1 isoform accounted for virtually all of the AMPK activity measurable in rat liver extracts and that alpha 2 was virtually inactive (16), but a subsequent study has challenged this finding (17). Immunoprecipitation of specific isoforms from rat liver show that both alpha 1 and alpha 2 contribute equally to AMPK activity and expression of recombinant enzyme demonstrate that both isoforms have comparable specific activities (17). The only difference that could be detected between the two isoforms was in their specificity for peptide substrates (17). Although subtle, this does raise the possibility that there could be differences in the downstream targets in vivo leading to different physiological roles for alpha 1 and alpha 2.

In an attempt to identify novel downstream targets of AMPK, we screened a two-hybrid library with AMPKalpha 2 as bait. Here, we report the identification and characterization of a second isoform for AMPKbeta , which is highly expressed in skeletal muscle. Comparison of the amino acid sequences of the two beta  isoforms with Sip2p and Gal83p reveal two highly conserved regions, which are predicted to interact with the alpha  and gamma  subunits. The sequences of the beta isoforms diverge at their N termini, suggesting that this region may play an important role in conferring isoform specificity, either by targeting to different substrates and/or intracellular locations or by responding to different stimuli.

    EXPERIMENTAL PROCEDURES
Top
Abstract
Introduction
Procedures
Results
Discussion
References

Materials-- A human skeletal muscle library in pGAD10 was obtained from CLONTECH. pYTH9 used to make a fusion of AMPKalpha 2 with the DNA-binding domain of Gal4 was a gift from Dr. Julia White, GlaxoWellcome Research Group. Yeast strain Y190 was used for the two-hybrid screening, and standard methods were used for manipulation and growth of yeast (18). Oligonucleotides were obtained from Genosys. CCL13 cells were obtained from the American Type Culture Collection and grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum.

Plasmid Construction-- In order to remove an internal XbaI site, AMPKalpha 2 cDNA was amplified by the polymerase chain reaction (PCR) with the following oligonucleotides: AGATCGGACACTACGTG (forward primer) and CCTCATCATCGATGCTTTTAAAGTCCAGAAG (reverse primer). The amplified product was digested with ClaI and BglII and ligated with AMPKalpha 2 (ClaI/BglII). A second round of amplification was carried out on the modified AMPKalpha 2 cDNA using the following primers: GAGCTCAGGTCGACGCCCATGGCTGAGAAGC and TCTGTCAGGAATTCAGAAAGACAGAGACCG. The product was digested with SalI and EcoRI (restriction sites shown underlined in the primers) and cloned into the SalI-EcoRI sites of plasmid pYTH9 to yield pYTH9alpha 2. For mammalian cell expression, AMPK subunit cDNAs were cloned into pCDNA3 expression vector (Invitrogen). Plasmid DNA was prepared using a QIAGEN maxiprep kit according to the manufacturer's instructions.

Two-hybrid Library Screening-- The vector pYTH9alpha 2 was linearized with XbaI and integrated into the genome of yeast strain Y190. Competent cells were transformed with a human skeletal muscle library in pGAD10 and plated on selective medium lacking histidine, leucine, and tryptophan. After incubation for 10 days at 30 °C, filter lifts of the colonies were taken using Whatman No. 3MM paper and replicated onto fresh plates before being permeabilized by immersing twice in liquid nitrogen for 5 s. Filters were incubated with 5-bromo-4-chloro-3-indoyl beta -D-galactoside at room temperature for 1 h, and positive clones were identified by the appearance of blue colonies. The position of the positive colonies was marked on the replica plates, and these were incubated overnight at 30 °C. Positive clones were individually transferred to plates lacking leucine and tryptophan in order to isolate single colonies. Where appropriate, plasmid DNA was extracted from 1.5-ml yeast cultures and used to transform Escherichia coli.

Analysis of Positive Clones-- cDNA inserts in pGAD10 were amplified from yeast extracts by PCR using primers GADF1 (GATAAGATACCCCACCAAACCC) and GADR1 (CTTGCGGGGTTTTTCAGTATCTACG) flanking the cloning sites. Following an extended hot start of 10 min at 95 °C, 35 cycles of 95 °C for 1 min, 56 °C for 1 min, and 72 °C for 1 min were used. Products were analyzed by electrophoresis either before or after digestion with either RsaI, Sau3A, and HaeIII. Clones were grouped according to their restriction digest pattern, and two separate clones from each group were isolated from the original yeast colonies and transformed into E. coli. The inserts were sequenced by the dideoxy chain termination method (19) using Sequenase (version 2.0 from Amersham Pharmacia Biotech) in accordance with the manufacturer's instructions.

Identification of Human AMPKbeta 1 cDNA-- The nucleotide sequence of rat AMPKbeta (10) was used to search the dbEST (Expressed Sequence Tag) data base using the BLAST (Basic Local Alignment Search Tool) program. Two clones (accession numbers H06094 and R20494) were obtained from the IMAGE consortium. Plasmid DNA from the clones was prepared and the inserts sequenced.

Northern Blot Analysis-- A human multiple tissue Northern blot (CLONTECH) was probed with either random-primed human AMPKbeta 1 cDNA or AMPKbeta 2 cDNA. Following hybridization the blots were washed with 2 × SSC, 0.5% SDS at room temperature for 1 h, followed by 0.2 × SSC, 0.5% SDS at 60 °C for 2 × 1 h, and autoradiographed for 1-5 days at -70 °C.

Antibody Production-- Peptides based on the amino acid sequence of rat AMPKbeta 1 (residues 20-33, PRRDSSGGTKDGDR) and human AMPKbeta 2 (residues 44-57, SVFSLPDSKLPGDK) were synthesized and coupled to keyhole-limpet hemocyanin via a cysteine residue added at the N terminus of the peptide. The conjugated peptides were used to immunize sheep (AMPKbeta 1-specific peptide) or rabbits (AMPKbeta 2-specific peptide). In some cases, antibodies were affinity-purified from serum using the appropriate peptide conjugated to thiol-Sepharose (20).

Immunoprecipitations-- Affinity-purified isoform-specific antibodies were bound to either protein A-Sepharose (rabbit antibodies) or protein G-Sepharose (sheep antibodies) and used to immunoprecipitate AMPK from rat tissue extracts. For comparison of AMPK in liver and skeletal muscle, AMPK was partially purified by chromatography on DEAE-Sepharose (21). A mouse monoclonal antibody (clone 9E10; Ref. 22) was bound to protein A-Sepharose and used to immunoprecipitate AMPK (in which both the alpha 1 and alpha 2 subunits have the myc peptide EQKLISEEDL added at the N terminus) from transiently transfected CCL13 cells. Extracts were precleared by incubation with rabbit preimmune serum bound to protein A-Sepharose for 2 h at 4 °C and then incubated with an excess of the appropriate antibody for 2-16 h at 4 °C. Immune complexes were collected by centrifugation at 6000 × g for 5 min, washed extensively with buffer A (50 mM Tris-HCl, pH 7.5, 50 mM NaF, 5 mM sodium pyrophosphate, 1 mM EDTA, 1 mM dithiothreitol, 10% (v/v) glycerol, 1% (v/v) Triton X-100), and then analyzed for AMPK activity and by Western blotting.

Western Blots-- Samples were resolved by SDS-PAGE and transferred to polyvinylidene difluoride membrane. The membrane was blocked by incubation in 10 mM Tris-HCl, pH 7.4, 0.5 M NaCl, 0.5% Tween 20, 5% low fat milk powder overnight at 4 °C. The membrane was then incubated with primary antibody in the same buffer for 2-4 h at room temperature. After extensive washing with 10 mM Tris-HCl, pH 7.4, 0.5 M NaCl, 0.5% Tween 20, the membrane was incubated for 1 h at room temperature with either protein A conjugated with horseradish peroxidase (for primary antibodies raised in rabbits) or protein G conjugated with horseradish peroxidase (for sheep antibodies). After further extensive washing, the membrane was developed using enhanced chemiluminescence (Boehringer Mannheim).

In Vitro Translations-- cDNAs encoding AMPKbeta isoforms were constructed in pCDNA3 (Invitrogen). RNA transcripts were synthesized using T7 polymerase and translated in reticulocyte lysates using a coupled transcription/translation system (TNT system, Promega) in the presence of [35S]methionine. Total labeled products of translation were analyzed by SDS-PAGE and fluorography.

Mammalian Cell Transfections-- For mammalian expression all AMPK cDNAs were constructed in pCDNA3. cDNAs encoding alpha 1 and alpha 2 were constructed with a sequence encoding a 10-amino acid epitope tag derived from c-Myc (EQKLISEEDL; Ref. 22) immediately following the initiating methionine. CCL13 cells were transfected with plasmid (10 µg of each plasmid) by calcium phosphate precipitation (23). The precipitate was incubated with the cells overnight, followed by a 2-min incubation with phosphate-buffered saline containing 10% (v/v) dimethyl sulfoxide. Cells were harvested 60 h after transfection and lysed in buffer A. Insoluble material was removed by centrifugation and the supernatant analyzed by Western blotting or used for immunoprecipitation using anti-AMPK antibodies or an anti-Myc antibody (22).

AMPK Activity-- Activity was measured by phosphorylation of the SAMS peptide as described previously (24).

    RESULTS
Top
Abstract
Introduction
Procedures
Results
Discussion
References

Identification of a Novel AMPKbeta Subunit Using the Two-hybrid System-- In order to identify proteins interacting with the catalytic subunit of AMPK, we screened a human skeletal muscle two-hybrid library with AMPKalpha 2 as bait. A total of 2.3 million clones were screened, and 136 positive clones were identified by blue/white selection from the lacZ reporter gene. Grouping of the clones by restriction digest mapping and partial sequencing of the cDNA inserts revealed that 50 clones, which we designate AMPKbeta 2, shared considerable sequence identity with the cDNA encoding the rat AMPKbeta 1 subunit. A representative clone was sequenced in its entirety, and the nucleotide and predicted amino acid sequences are shown in Fig. 1A. The nucleotide sequence of beta 2 is 66.7% identical to rat beta 1, whereas the predicted amino acid sequence is 70.6% identical to the rat sequence (10). In order to determine whether the cDNA we had isolated was the human homologue of rat beta 1, we searched the human EST data base with the rat beta 1 and human beta 2 sequences. A number of clones were identified with sequences, which, although highly related to rat beta 1, did not match beta 2. Two of these clones (with accession numbers H06094 and R20494) encoded the sequence corresponding to the N-terminal region of rat beta 1 (including the initiating methionine) and were obtained from the IMAGE consortium through the UK Human Genome Mapping Resource Center, Cambridge. Sequence analysis of these clones revealed that they were identical, except that clone H06094 had a 343-base pair deletion compared with clone R20494 (see Fig. 1B). The nucleotide and predicted amino acid sequence of clone R20494, which we designate human beta 1, is shown in Fig. 1B. At the nucleotide level, human beta 1 is 89% identical to rat beta 1 (97% amino acid sequence identity) and 66% identical to human beta 2. These results indicate that clone R20494 is the human homologue of rat beta 1 and that beta 2, therefore, is a novel beta  subunit isoform.


View larger version (36K):
[in this window]
[in a new window]
 
Fig. 1.   Nucleotide sequence and predicted amino acid sequence of human AMPKbeta 1 and AMPKbeta 2. A, beta 2. The initiating and stop codons are shown in bold. Nucleotides are numbered on the right and amino acids on the left. B, beta 1. Arrows mark the limits of the 343-base pair deletion found in clone H06094.

The predicted amino acid sequences of human beta 1 and beta 2 are 71% identical and are shown aligned in Fig. 2A. The two sequences are most divergent in the region spanning the N-terminal 75 amino acids (40% identity), although a predicted N-myristoylation signal is conserved in both isoforms (25), while the remaining C-terminal sequence is highly conserved. We have previously shown that rat beta 1 shares sequence identity with a family of yeast proteins that interact with Snf1p, the yeast homologue of AMPKalpha (10). Fig. 2B shows the amino acid sequence alignment of the conserved regions of two members of this family (Sip2p and Gal83p) with human beta 1 and beta 2. Two regions that show a strong degree of sequence conservation between all four proteins can be identified, corresponding to amino acid residues 79-155 and 203-270 in beta 1.


View larger version (82K):
[in this window]
[in a new window]
 
Fig. 2.   Amino acid sequence alignments of the beta  isoforms showing the regions of conservation with the yeast proteins, Sip2p and Gal83p. A, the deduced amino acid sequences of human beta 1 (top) and beta 2 (bottom) were aligned using the GAP program in the University of Wisconsin package with a gap weight of 3.0 and a length weight of 0.1. Dots indicate gaps introduced to maximize the alignment. Identities between the two sequences are boxed. B, the amino acid sequences of beta 1, beta 2, Sip2p, and Gal83p were aligned as above, using the PILEUP program. Numbers on the left refer to amino acid residues within the full-length polypeptides. The N-terminal regions showed no significant homology and have been omitted. A solid bar beneath the sequences marks the two highly conserved regions, which are contained within the KIS and ASC domains of Sip2p and Gal83p (15).

Tissue Distribution of AMPKbeta 1 and AMPKbeta 2-- Poly(A)-rich RNA isolated from a number of human tissues was probed with cDNA encoding beta 1 or beta 2 and the results are shown in Fig. 3. beta 1 mRNA is detected as a single band of approximately 3 kilobase pairs and is expressed in all tissues examined at approximately equal levels. A single band of approximately 7.5 kilobase pairs is detected with the beta 2 probe, although in this case there is clearly a difference in the pattern of expression. Relatively high levels of expression are detected in skeletal and cardiac muscle with low levels in the kidney and lung.


View larger version (41K):
[in this window]
[in a new window]
 
Fig. 3.   Northern blot analysis of beta 1 and beta 2. A human multiple tissue Northern blot (CLONTECH) was hybridized with a cDNA probe encoding either beta 1 or beta 2. In each case the blot was washed with 0.2 × SSC, 0.5% SDS at 60 °C and exposed at -70 °C. The migration of RNA standards is indicated.

Antibodies were raised against specific peptide sequences derived from either beta 1 or beta 2 and used to determine protein expression in a number of rat tissues. Preliminary experiments revealed that it was not possible to detect the subunits directly by Western blotting of tissue lysates (data not shown), and so tissue homogenates were first immunoprecipitated using the antibodies bound to protein A- or protein G-Sepharose and then the immune complexes were analyzed by SDS-PAGE, followed by Western blotting. Fig. 4A shows the expression pattern of the two beta  isoforms. The very prominent band migrating at approximately 56 kDa, detected in both blots, is due to a strong cross-reaction of the IgG heavy chains, present from the immunoprecipitation, with the protein A/G-conjugated horseradish peroxidase used for detection of the blotting antibody. In each case, however, an additional band specific for the beta  isoform, migrating ahead of the IgG band, can be detected. beta 1 expression is highest in the liver and brain with low level expression in kidney and skeletal muscle, whereas beta 2 is most highly expressed in skeletal muscle with low level expression in kidney, liver, and lung. At the present time, we cannot explain why beta 2 appears as a distinct doublet in lung. Although the predicted molecular mass of each isoform is 30 kDa, both beta 1 and beta 2 migrate anomalously on SDS-PAGE. beta 1, as we have reported previously, migrates with an apparent molecular mass of approximately 38 kDa (10), whereas beta 2 migrates with an apparent molecular mass of approximately 34 kDa (Fig. 4A). In vitro translation of beta 1 and beta 2 in a rabbit reticulocyte lysate system results in a similar discrepancy in the migration of the isoforms on SDS-PAGE (Fig. 4B). We have not been able to determine the reason for the anomalous migration of the beta  isoforms on SDS-PAGE.


View larger version (40K):
[in this window]
[in a new window]
 
Fig. 4.   Expression of beta  isoforms in rat tissues. A. Western blot analysis. Immune complexes, following immunoprecipitation of the indicated tissue extracts using antibodies specific for either beta 1 or beta 2, were resolved by SDS-PAGE. Proteins were transferred to a polyvinylidene difluoride membrane and probed with either beta 1- or beta 2-specific antibodies. The blotting antibody was detected with either protein G (beta 1) or protein A (beta 2) conjugated to horseradish peroxidase and visualized by enhanced chemiluminescence. Bands corresponding to beta 1 and beta 2 are marked by an arrow. Migration of molecular mass standards is indicated. B, [35S]methionine-labeled beta 1 or beta 2 was translated in vitro in rabbit reticulocyte lysate programmed with RNA synthesized from beta 1 or beta 2 cDNA in pCDNA3. Proteins were resolved by SDS-PAGE and labeled products detected by fluorography. Migration of molecular mass standards is indicated. C, AMPK was immunoprecipitated from partially purified extracts of rat liver (open symbols) or skeletal muscle (closed symbols) using antibodies specific for beta 1 (squares) or beta 2 (circles) and activity in the immune complexes determined. In order to obtain a linear rate of phosphorylation, 50-fold less protein (10 µg) was used for immunoprecipitation of beta 1 from liver. D, the immune complexes from C were resolved by SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and probed with either alpha 1- or alpha 2-specific antibodies.

It is clear from the Western blots that there is a marked difference in the relative expression of beta 1 and beta 2 in the liver compared with skeletal muscle. In order to investigate this difference in more detail, AMPK was partially purified from either rat liver or skeletal muscle by ion-exchange chromatography on DEAE-Sepharose (21) and immunoprecipitated using beta 1- or beta 2-specific antibodies. AMPK activity in the immune complexes was determined and is shown in Fig. 4C. AMPK activity is present in the immune complex following immunoprecipitation of liver or skeletal muscle extracts with either beta 1- or beta 2-specific antibodies. In liver, however, much higher activity is recovered with the beta 1-specific antibody, and in order to obtain a linear rate of phosphorylation it was necessary to use a 50-fold lower amount of liver extract when immunoprecipitating with beta 1 compared with beta 2. Despite using a lower amount of starting material, AMPK activity in the beta 1 immune complex is still greater than in the beta 2 complex (Fig. 4C), suggesting that nearly all the activity in liver is associated with the beta 1 isoform. In contrast, immunoprecipitation from a skeletal muscle extract with either beta 1 or beta 2 yields approximately equal activities. Following determination of AMPK activity, the immune complexes were analyzed by Western blotting using alpha 1- or alpha 2-specific antibodies. Both beta  isoforms form complexes with alpha 1 and alpha 2 in liver and skeletal muscle (Fig. 4D). Although the blots are not quantitative, it is possible to draw some general conclusions from them regarding the relative amounts of the different AMPK complexes present in the two tissues. In liver the beta 1 complexes (both alpha 1beta 1gamma and alpha 2beta 1gamma ) are more abundant than the beta 2 complexes (compare lanes 1 and 3 in Fig. 4D), consistent with the finding that most of the AMPK activity can be immunoprecipitated with beta 1-specific antibodies. In skeletal muscle the reverse is true, with the beta 2 complexes being more abundant than the beta 1 complexes (compare lanes 2 and 4). In this case, however, AMPK activity following immunoprecipitation with either beta 1 or beta 2 is approximately the same, although significantly lower than the corresponding activities from liver (Fig. 4C).

Expression of Subunits in Mammalian Cells-- cDNAs encoding alpha 1/beta 1/gamma 1, alpha 1/beta 2/gamma 1, alpha 2/beta 1/gamma 1, or alpha 2/beta 2/gamma 1 were co-transfected into CCL13 cells. AMPK was immunoprecipitated from transfected cells with an anti-Myc antibody (a Myc epitope tag is present on both alpha 1 and alpha 2) and kinase activity in the immune complexes determined. No significant differences in AMPK activity between beta 1-containing complexes compared with beta 2-containing complexes were detected (Fig. 5A). As has been reported previously, complexes containing alpha 1 were expressed at higher levels than those containing alpha 2 and yield correspondingly higher activities (7). The immune complexes were analyzed by Western blotting using an antibody that recognizes both beta  isoforms (10) (Fig. 5B). Transfections with beta 1 yield a single band migrating with an apparent molecular mass of approximately 38 kDa, whereas transfections with beta 2 reveal a single band migrating with an apparent molecular mass of 34 kDa. No products could be detected in untransfected cells. There is no significant difference in the Western blots from immunoprecipitates of alpha 1- or alpha 2-transfected cells, indicating that beta 1 and beta 2 can form complexes with either alpha 1 or alpha 2, consistent with the results from liver and skeletal muscle. As we have reported previously, co-transfection of alpha , beta , and gamma  is required in order to detect expression of recombinant protein or activity in this system (10) (data not shown).


View larger version (23K):
[in this window]
[in a new window]
 
Fig. 5.   Expression of beta  isoforms in mammalian cells. A, AMPK activity determined in immune complexes of CCL13 cells following transient transfection with cDNAs encoding alpha 1beta 1gamma 1 (open circles), alpha 1beta 2gamma 1 (closed circles), alpha 2beta 1gamma 1 (open squares), or alpha 2beta 2gamma 1 (closed squares) is shown. Note the difference in scale between the activities of alpha 1 and alpha 2. B, the immune complexes from A were resolved by SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and probed with an antibody that recognizes both beta  isoforms. Migration of molecular mass standards is indicated.

    DISCUSSION
Top
Abstract
Introduction
Procedures
Results
Discussion
References

We report here the identification of a second isoform of AMPKbeta (termed beta 2), which we isolated from a two-hybrid screen for proteins interacting with the catalytic subunit of AMPK (alpha 2). cDNA encoding the human homologue of rat beta 1 was identified from a search of the EST data base, and a clone (accession number R20494) containing the entire coding sequence was obtained from the IMAGE consortium. In a recent paper, it was reported that at least three distinct gene products for AMPKbeta were represented in the EST data base (16). Our results do not support this claim, indicating that all of the beta  sequences in the EST data base correspond to either beta 1 or beta 2. It is worth noting, however, that most of the entries in the data base do not match exactly the sequences of beta 1 and beta 2 reported here, differing by a number of single base changes. We believe that these differences probably represent errors in the automated DNA sequence analysis used to generate the EST sequences. The majority of the discrepancies occur at the beginning and end of the EST sequences, which are more prone to reading errors. At the present time, therefore, we believe that only two beta  isoforms can be identified, although we do not exclude the possibility that additional isoforms exist (see also "Addendum"). In addition to the beta  subunit isoforms, two isoforms of the alpha  subunit have been characterized (16). Searching of the EST data base, combined with preliminary results obtained from two-hybrid screening using the beta 1 subunit as bait, demonstrate that there are at least three genes coding for isoforms of the gamma  subunit.2 The existence of different isoforms for each of the AMPK subunits suggests that a large subfamily of AMPK complexes is present in mammals (see below).

The amino acid sequences of beta 1 and beta 2 share considerable identity with a family of related proteins in yeast, which interact with the SNF1 protein kinase complex (10, 11, 16, 27, 28). Amino acid sequence alignment of beta 1, beta 2, Sip2p, and Gal83p reveals two highly conserved regions (Fig. 2B), which have been shown recently to be important in mediating the interaction of the yeast proteins with Snf1p and Snf4p (15). The conserved C-terminal region, termed the ASC domain, interacts with Snf4p, while the conserved internal region, termed the KIS domain, interacts with Snf1p (15). We have shown previously that the beta  subunit plays an important role in the formation of the ternary AMPK complex in vitro since it interacts with both alpha  and gamma , whereas alpha  and gamma do not appear to interact directly (10). The simplest interpretation of these results is that the alpha  and gamma  subunits bind independently to the beta  subunit, probably through the two regions related to the KIS and ASC domains in the yeast proteins. It remains to be determined whether binding of the alpha  and gamma  subunits to beta  then allows them to interact directly with one another, although there is evidence that this may be the case with the homologous subunits in the yeast complex (15). Studies in our laboratory are currently under way to examine these interactions in more detail. It is unlikely that the sole purpose of the beta  subunit is to mediate the formation of the AMPK complex, since both isoforms appear equally competent to perform this function in vitro and in vivo. Most of the amino acid sequence variation between beta 1 and beta 2 occurs within the N-terminal region, and this is also the case for Sip2p and Gal83p (data not shown). It seems likely, therefore, that functional differences between the beta  isoforms will be attributable to the divergent N-terminal regions and will not entail assembly of the heterotrimeric complex.

Both beta  isoforms are expressed in a wide range of tissues, but there is a marked difference in their expression patterns. beta 1 is most highly expressed in liver with low level expression in skeletal muscle, and this pattern is reversed for beta 2. These findings suggest that the beta  isoforms may play tissue-specific roles in regulating the activity and/or function of AMPK. To begin to address these questions, we examined the effect of expression of the different beta  isoforms on AMPK activity in a recombinant system. Co-expression of the alpha  and gamma  subunits with either beta 1 or beta 2 in CCL13 cells did not reveal a significant difference in AMPK activity between the two beta  isoforms, although, as reported previously, expression with alpha 1 led to substantially higher activity than with alpha 2 (7). Furthermore, beta 1 and beta 2 both interact with alpha 1 and alpha 2, and we did not detect any obvious difference in the association of the different alpha  and beta  isoforms in vitro. These results imply that the beta  isoforms do not directly alter the activity of AMPK per se, but they do not exclude a less direct role in the regulation of AMPK. We decided therefore to examine the relative expression and activity of beta 1- and beta 2-containing AMPK in vivo, and since there was an obvious difference in the expression of the isoforms between the liver and skeletal muscle, we concentrated on these tissues. Complexes containing all four possible combinations of the alpha  and beta  isoforms were detected in liver and skeletal muscle, indicating that there are no constraints on isoform-specific subunit composition in vivo. In liver the beta 1-containing complexes account for virtually all of the AMPK activity, and this may simply reflect the relative abundance of the beta 1 complexes compared with beta 2. Consistent with this result is the finding that purification of AMPK from rat liver yields a preparation in which the beta  subunit is almost exclusively beta 1, as judged by migration on SDS-PAGE and amino acid sequencing (9, 10, 26). It is clear from our results, however, that a proportion of AMPK from rat liver contains the novel beta 2 isoform. In skeletal muscle, although there is more beta 2 complex compared with beta 1, AMPK activity associated with the different beta  isoforms is approximately the same. These results suggest that the beta 2 complex, or a proportion of it, is in a relatively inactive state compared with the beta 1 complex. We reported previously that AMPK isolated from skeletal muscle is in a relatively inactive state and suggested that this could be due to lack of association of the alpha  subunit with the beta  and gamma  subunits (29). The results of our present study appear to rule out this possibility, and we are currently investigating the basis for the low activity state of AMPK in muscle.

We have detected the gamma  subunit in all of the complexes isolated in vivo, although we have not been able to determine which particular isoform is present in the different complexes. AMPK isolated from liver contains predominantly the gamma 1 isoform, as judged by amino acid sequencing (9, 10, 26), although the gamma 2 and gamma 3 isoforms are also expressed in liver (25).2 It is likely that, at least in liver, some complexes will contain the gamma 2 and gamma 3 isoforms.

We believe that our results provide valuable clues regarding the possible role of the beta  subunit in the regulation of AMPK. The marked difference in the expression patterns of beta 1 and beta 2 in liver and skeletal muscle strongly suggests a tissue-specific role for the isoforms. It was reported recently that AMPKalpha 2, but not AMPKalpha 1, is activated in response to increased contraction in rat skeletal muscle (30). Although we have not assessed the relative amounts of the alpha  isoforms in skeletal muscle, the predominant beta  isoform is beta 2. Interestingly, however, both beta 1 and beta 2 account for approximately equal AMPK activity in muscle. It is possible that association with the different beta  isoforms could play a part in an altered response to the upstream signaling pathways that lead to AMPK activation. This could explain the differential activation of alpha 2 in skeletal muscle during contraction if there were a pool of inactive alpha 2beta 2-containing AMPK. Subtle differences in substrate recognition have been reported between alpha 1 and alpha 2, suggesting that the isoforms could phosphorylate different proteins, at different rates, within the cell (17, 31). The ability of the different isoform complexes to have a varying response to specific stimuli, coupled with the differing substrate specificity between alpha 1 and alpha 2, would allow an enormous degree of flexibility within the AMPK cascade.

    ACKNOWLEDGEMENT

We are grateful to Julia White for help with the two-hybrid screening.

    Addendum

During the preparation of this manuscript, Stapleton et al. reported the partial sequence of a mouse cDNA identified in the EST data base (accession number W07176), which corresponds to the mouse homologue of beta 2 (26).

    FOOTNOTES

* This work was supported by the Medical Research Council (United Kingdom) and by a studentship from GlaxoWellcome (to C. T.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AJ224515 and AJ224538.

§ To whom correspondence should be addressed: MRC Clinical Sciences Centre, Cellular Stress Group, Imperial College School of Medicine, Hammersmith Hospital, DuCane Rd., London W12 0NN, United Kingdom. Tel.: 44-181-383-4314; Fax: 44-181-383-2028; E-mail: dcarling{at}rpms.ac.uk.

1 The abbreviations used are: AMPK, AMP-activated protein kinase; AMPKK, AMP-activated protein kinase kinase; EST, expressed sequence tag; PAGE, polyacrylamide gel electrophoresis; PCR, polymerase chain reaction; SAMS, the synthetic peptide substrate with the sequence HMRSAMSGLHLVKRR.

2 P. C. F. Cheung and D. Carling, manuscript in preparation.

    REFERENCES
Top
Abstract
Introduction
Procedures
Results
Discussion
References

  1. Corton, J. M., Gillespie, J. G., and Hardie, D. G. (1994) Curr. Biol. 4, 315-324[Medline] [Order article via Infotrieve]
  2. Hardie, D. G., Carling, D., and Halford, N. (1994) Semin. Cell Biol. 5, 409-416[CrossRef][Medline] [Order article via Infotrieve]
  3. Hardie, D. G. (1994) Cell. Signalling 6, 813-821[CrossRef][Medline] [Order article via Infotrieve]
  4. Hardie, D. G., and Carling, D. (1997) Eur. J. Biochem. 246, 259-273[Abstract]
  5. Hawley, S. A., Selbert, M. A., Goldstein, E. G., Edelman, A. M., Carling, D., and Hardie, D. G. (1995) J. Biol. Chem. 270, 27186-27191[Abstract/Free Full Text]
  6. Carling, D., Aguan, K., Woods, A., Verhoeven, A. J. M., Beri, R. K., Brennan, C. H., Sidebottom, C., Davison, M. D., and Scott, J. (1994) J. Biol. Chem. 269, 11442-11448[Abstract/Free Full Text]
  7. Dyck, J. R. B., Gao, G., Widmer, J., Stapleton, D., Fernandez, C. S., Kemp, B. E., and Witters, L. A. (1996) J. Biol. Chem. 271, 17798-17803[Abstract/Free Full Text]
  8. Mitchelhill, K. I., Stapleton, D., Gao, G., House, C., Michell, B., Katsis, F., Witters, L. A., and Kemp, B. E. (1994) J. Biol. Chem. 269, 2361-2364[Abstract/Free Full Text]
  9. Stapleton, D., Gao, G., Michell, B. J., Widmer, J., Mitchelhill, K., Teh, T., House, C. M., Witters, L. A., and Kemp, B. E. (1994) J. Biol. Chem. 269, 29343-29346[Abstract/Free Full Text]
  10. Woods, A., Cheung, P. C. F., Smith, F. C., Davison, M. D., Scott, J., Beri, R. K., and Carling, D. (1996) J. Biol. Chem. 271, 10282-10290[Abstract/Free Full Text]
  11. Celenza, J. L., and Carlson, M. (1986) Science 233, 1175-1180[Medline] [Order article via Infotrieve]
  12. Gao, G., Fernandez, C. S., Stapleton, D., Auster, A. S., Widmer, J., Dyck, J. R. B., Kemp, B. E., and Witters, L. A. (1996) J. Biol. Chem. 271, 8675-8681[Abstract/Free Full Text]
  13. Woods, A., Munday, M. R., Scott, J., Yang, X., Carlson, M., and Carling, D. (1994) J. Biol. Chem. 269, 19509-19515[Abstract/Free Full Text]
  14. Jiang, R., and Carlson, M. (1996) Genes Dev. 10, 3105-3115[Abstract]
  15. Jiang, R., and Carlson, M. (1997) Mol. Cell. Biol. 17, 2099-2106[Abstract]
  16. Stapleton, D., Mitchelhill, K. I., Gao, G., Widmer, J., Michell, B. J., Teh, T., House, C. M., Fernandez, C. S., Cox, T., Witters, L. A., and Kemp, B. E. (1996) J. Biol. Chem. 271, 611-614[Abstract/Free Full Text]
  17. Woods, A., Salt, I., Scott, J., Hardie, D. G., and Carling, D. (1996) FEBS Lett. 397, 347-351[CrossRef][Medline] [Order article via Infotrieve]
  18. Sherman, F. (1991) Methods Enzymol. 194, 3-21[Medline] [Order article via Infotrieve]
  19. Sanger, F., Nicklen, S., and Coulson, A. R. (1977) Proc. Natl. Acad. Sci. U. S. A. 74, 5463-5467[Abstract]
  20. Duggan, M. J., and Stephenson, A. F. (1990) J. Biol. Chem. 265, 3831-3835[Abstract/Free Full Text]
  21. Carling, D., Clarke, P. R., Zammit, V. A., and Hardie, D. G. (1989) Eur. J. Biochem. 186, 129-136[Abstract]
  22. Evans, G. I., Lewis, G. K., Ramsay, G., and Bishop, B. M. (1985) Mol. Cell. Biol. 5, 3610-3616[Medline] [Order article via Infotrieve]
  23. Chen, C., and Okayama, H. (1987) Mol. Cell. Biol. 7, 2745-2752[Medline] [Order article via Infotrieve]
  24. Davies, S. P., Carling, D., and Hardie, D. G. (1989) Eur. J. Biochem. 186, 123-128[Abstract]
  25. Johnson, R. D., Bhatnagar, R. S., Knoll, L. J., and Gordon, J. I. (1994) Annu. Rev. Biochem. 63, 869-914[CrossRef][Medline] [Order article via Infotrieve]
  26. Stapleton, D., Woollatt, E., Mitchelhill, K. I., Nicholl, J. K., Fernandez, C. S., Michell, B. J., Witters, L. A., Power, D. A., Sutherland, G. R., and Kemp, B. E. (1997) FEBS Lett. 409, 452-456[CrossRef][Medline] [Order article via Infotrieve]
  27. Celenza, J. L., Eng, F. J., and Carlson, M. (1989) Mol. Cell. Biol. 9, 5045-5054[Medline] [Order article via Infotrieve]
  28. Yang, X., Jiang, R., and Carlson, M. (1994) EMBO J. 13, 5878-5886[Abstract]
  29. Verhoeven, A. J. M., Woods, A., Brennan, C. H., Hawley, S. A., Hardie, D. G., Scott, J., Beri, R. K., and Carling, D. (1995) Eur. J. Biochem. 228, 236-243[Abstract]
  30. Vavvas, D., Apazidis, A., Saha, A. K., Gamble, J., Patel, A., Kemp, B. E., Witters, L. A., and Ruderman, N. B. (1997) J. Biol. Chem. 272, 13255-13261[Abstract/Free Full Text]
  31. Michell, B. J., Stapleton, D., Mitchelhill, K. I., House, C. M., Katsis, F., Witters, L. A., and Kemp, B. E. (1996) J. Biol. Chem. 271, 28445-28450[Abstract/Free Full Text]


Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.