From the Department of Pathology and Microbiology,
University of Nebraska Medical Center, Omaha, Nebraska 68198-6495, the ** Department of Biochemistry, University of Bath, Bath BA2 7AY,
United Kingdom, and the
Department of Pathology and Laboratory
Medicine, University of Texas Health Sciences Center,
Houston, Texas 77030
![]() |
ABSTRACT |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Activating transcription factor 1 (ATF1) and
cAMP-responsive element (CRE)-binding protein (CREB) activate
transcription through CREs located in the promoters of cellular and
viral genes. We previously described a monoclonal antibody (mAb41.4)
that prevents ATF1 binding to DNA and reduces CRE-driven promoter
activity in vitro (Orten, D. J., Strawhecker, J. M., Sanderson, S. D., Huang, D., Prytowsky, M. B., and
Hinrichs, S. H. (1994) J. Biol. Chem. 269, 32254-32263). A single chain Fv (scFv) fragment from the mAb41.4-expressing hybridoma was generated to provide a means to
investigate transcription factor function via intracellular expression
of the scFv fragment. The affinity of scFv4 (subgroup: VL
-III, VH miscellaneous) for ATF1 was similar to that of
the parental mAb and the Fab fragment, but it demonstrated greater inhibitory activity and reacted with CREB. scFv4 disrupted the binding
of both ATF1 and CREB in electrophoretic mobility shift assays and
reduced expression of CRE-driven expression in vitro. Transient expression of scFv had no effect on the non-CRE-containing adenovirus major late promoter. The proliferating cell nuclear antigen
promoter, containing two CREs, was significantly more sensitive to
inhibition by scFv than the cytomegalovirus immediate-early promoter,
containing five CREs. Cotransfection of either ATF1 or CREB in the
presence of scFv restored basal levels of expression. The intracellular
expression of scFv provides a unique means to investigate the roles of
the transcription factors ATF1 and CREB.
![]() |
INTRODUCTION |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Activating transcription factor 1 (ATF1)1 and cAMP-responsive element-binding protein (CREB) are members of the large bZIP superfamily of transcription factors. Members of the CREB/ATF family bind to cAMP-responsive elements (CREs) within the promoter and enhancer sequences of many genes. ATF1, CREB, and the cAMP-responsive element modulator protein (CREM) constitute the CREB/ATF subfamily within the bZIP superfamily, which is defined by their ability to heterodimerize with each other but not with members of other subfamilies (1). ATF1, CREB, and CREM have similar structure and are highly homologous at the amino acid sequence level, especially within the bZIP region. Despite these similarities, members of the CREB multigene subfamily have distinct biological activity.
ATF1, CREB, and CREM may act as either positive or negative regulators of transcription. Through alternative mRNA splicing, numerous isoforms of ATF1, CREB, and CREM can be expressed that possess differing transcriptional regulatory activities (2, 3). Each protein or isoform also possesses differing patterns of phosphorylation, and the specific patterns contribute to their activity as regulators of transcription (1). CREB and CREM have been shown to play pivotal roles in basal and hormone-regulated transcription and differentiation; the role of ATF1 is less well defined. ATF1 homodimers appear to be weaker transcriptional activators than either CREB or certain forms of CREM since ATF1-mediated activation is enhanced by heterodimerization with either CREB or CREM (2). The overexpression of ATF1 has recently been correlated with the neoplastic phenotype of lymphoma (4) and the continuous proliferation of lymphocytes. Cell cycle progression in T cells stimulated by interleukin-2 has been shown to be regulated by ATF1 and CREB, and transgenic mice expressing a dominant-negative form of CREB are defective in thymocyte proliferation and interleukin-2 production (5, 6).
To investigate the functional role of ATF1, we previously generated a
panel of anti-ATF1 monoclonal antibodies (7). One antibody (mAb41.4;
hereafter mAb4) was found to inhibit the binding of DNA by ATF1 and to
inhibit ATF1-induced transcriptional activation (7). Fab4, the Fab
fragment of mAb4, retained the inhibitory properties of the parental
antibody (Ab) (8). To investigate the cellular role of ATF1, we
utilized current advances in the engineering of antibodies that have
made possible the cloning of small single chain Fv (scFv) fragments.
scFv fragments contain the antigen-binding variable domains of the
light and heavy chains connected by a peptide spacer (9-11). When
constructed in this manner, a single RNA transcript can be expressed
and translated into an active protein that has the potential to
interfere with the activity of targeted intracellular proteins.
Intracellular scFv fragments have been successfully employed to
decrease the expression of ErbB-2 (12, 13); the subunit of the high
affinity human interleukin-2 receptor (14); and proteins of human
immunodeficiency virus type 1, including Rev, reverse transcriptase,
and envelope protein gp120 (15-18). We therefore cloned scFv4 of mAb4
and present here its characterization and its functional effect on
intracellular gene expression.
![]() |
EXPERIMENTAL PROCEDURES |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Preparation of mAb and Fab Fragments-- Preparations of mAb4 were affinity-purified on a protein G column and quantitated by absorbance at 280 nm (assuming 1 mg of IgG/ml of IgG = 1.4 A280) and the Bradford assay (Bio-Rad). Fab fragments were generated as described previously (8). Purified mAb4 was incubated with papain-agarose beads, after which the digested Ab solution was purified away from Fc fragments by protein A column chromatography. Purified Fab4 was dialyzed into phosphate-buffered saline and concentrated by centrifugal ultrafiltration.
Cloning of scFv-- The single chain Fv fragment of mAb4 was cloned utilizing a modification of the procedure described by Winter and Milstein (11) and components of the Recombinant Phage Antibody system (Amersham Pharmacia Biotech). Total RNA was isolated from the mAb41.4 hybridoma and reverse-primed. The heavy and light variable regions (VH and VL, respectively ) were amplified in two separate reactions using degenerate primers (Amersham Pharmacia Biotech) corresponding to the framework regions bracketing the complementarity-determining regions (CDRs) of the VH and VL domains. The resulting polymerase chain reaction products were joined by a linker that encoded a flexible 15-amino acid chain of (Gly4-Ser)3 (Amersham Pharmacia Biotech). The linkage reaction thus generated the scFv cDNA encoding the VH-linker-VL domain. The cDNA product was subsequently cloned into the NotI and SfiI sites of the pCANTAB-5E phagemid vector (Amersham Pharmacia Biotech) as well as the pCANTAB5-H6 phagemid vector, which incorporates the c-myc antigen tag and a six-histidine tail (19).
Expression and Selection of scFv-- A recombinant phage antibody library was generated by transformation of Escherichia coli TG-1 cells with the phagemid vector containing the scFv cDNA products described above. Infection by M13-K07 helper phage allowed packaging of the recombinant phagemid into phage-expressing antibody. The production of antigen-reactive phage-displayed scFv was enriched through solid-phase panning of the phage library in tissue culture flasks coated with ATF1. Log-phase E. coli TG-1 cells were added to the panning flask and the ATF1-reactive phage-infected the cells. Individual plaques from the enriched phage library were subsequently screened by enzyme-linked immunosorbent assays (ELISAs) using recombinant ATF1 bound to the microtiter wells (7). Positive wells were detected with a horseradish peroxidase-conjugated anti-M13 Ab (Amersham Pharmacia Biotech). The phages capable of binding ATF1 were used to infect E. coli HB2151 for generation of soluble scFv secreted into the periplasm.
Production and Quantitation of Soluble scFv--
Soluble scFv
was produced and quantitated as described previously (20). E. coli HB2151 cells that had reached an A600
of 0.6 were induced with
isopropyl--D-thiogalactopyranoside and grown at 25 °C
for 4 h. The periplasm was extracted in a high salt lysate buffer,
clarified, and dialyzed. Yields averaged 1.5 mg of scFv/liter of
culture. scFv quantitation was performed through slot blotting of the
periplasmic extract and a peptide standard. The slot blots were stained
with an anti-c-myc tag Ab (murine 9E10 hybridoma, American
Type Culture Collection) and an alkaline phosphatase-conjugated
anti-mouse IgG heavy and light chain Ab (Jackson ImmunoResearch
Laboratories, Inc.). A standard curve (1-100 ng) using
c-myc-peptide-1 (Oncogene Scientific Inc.) was generated,
and the signal of scFv wells was visually compared for determination of
approximate concentration as well as digitally scanned for
densitometric analysis. Following normalization for mass (mass of
c-myc peptide = mass of scFv/8), the average
periplasmic concentration of scFv was observed to be 5 ng/µl.
Periplasmic extracts were used to characterize the activity of scFv in
ELISAs, Western blots, electrophoretic mobility shift assays (EMSAs), and in vitro transcription.
DNA Sequencing and Analysis-- Sequencing of the scFv inserts in selected clones was performed through automated sequencing (ABI Advanced Biotechnologies, Inc.). 5'- and 3'-pCANTAB sequencing primers (Amersham Pharmacia Biotech) that anneal to the linker region of the cloned scFv fragment and the poly-cloning site of the pCANTAB vector respectively, were used. The complete sequences of three scFv4 clones (C9, A6, and D6) were determined and compared to confirm the absence of copying errors using the MacVectorTM 3.5 software package (International Biotechnologies, Inc.). The nucleotide sequence of scFv4 was translated to amino acid sequence and analyzed using the AbM Version 2.01 software package (Oxford Molecular Ltd., Oxford, United Kingdom) (21-23). Computer analysis identified unusual residues in each Fv fragment and applied the Kabat definitions to define framework regions and CDRs of each Fv fragment. The antibody subgroup and family of each Fv fragment was determined.
ELISA-- The ability of scFv to bind ATF1 and CREB was compared with its parental mAb and Fab forms. Competitive ELISAs were performed using the scFv-containing periplasmic extracts and mAb4 or Fab4 as the initial antigen binder. Bound scFv was detected through the use of an anti-c-myc tag Ab and an alkaline phosphatase-conjugated anti-mouse heavy and light chain Ab. Bound mAb4 and Fab4 were detected directly with the anti-mouse heavy and light chain Ab. Competitive ELISAs utilized ATF1- or CREB-coated microtiter wells and full-length ATF1 or CREB and their synthetic subsequences as competitors (7). The competitor inhibitory peptides, peptides C and F, at concentrations of 0.1-10 µM were incubated with solutions of scFv4 periplasmic extract (diluted 1:5 in phosphate-buffered saline) or mAb4 or Fab4 (diluted to ~1 nM in phosphate-buffered saline) before addition to the ELISA plate wells. Negative controls included a competitor peptide unrelated to the sequences of ATF1 or CREB and a non-scFv-containing periplasmic extract generated from E. coli HB2151 cells transformed with the recircularized pCANTAB-5E parental vector (no insert). Results of competitive ELISAs performed with scFv, mAb, Fab, and negative controls were read on an ELISA plate reader at 405 nm and plotted as inhibitor concentration versus percent inhibition of binding.
Electrophoretic Mobility Shift Assays--
Electrophoretic
mobility shift assays were performed as described previously (7, 8).
32P-Labeled oligonucleotide containing the consensus CRE
(5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3') was incubated with either 50 ng of full-length recombinant ATF1 or CREB
or one of the deletion mutants: CREB (bZIP region amino acids
254-327) or
ATF2 (bZIP region amino acids 350-505) (Santa Cruz
Biotechnology). The binding reactions were carried out in the presence
or absence of mAb4, Fab4, scFv4, or the control E. coli.
periplasm. Following electrophoresis, the bound and unbound fractions
of labeled oligonucleotide were quantitated by autoradiography for
6-12 h using a PhosphorImager (Molecular Dynamics, Inc.). The
PhosphorImager data were exported as tagged image file format files to
Canvas 5 and used to prepare Fig. 3.
In Vitro Transcription--
The effects of scFv on transcription
were evaluated as described previously (8) using the HeLa cell nuclear
extract in vitro transcription system from Promega. The scFv
fragment, antibody, or control periplasm was incubated with nuclear
extract containing 5 mM MgCl2 for 30 min before
adding ribonucleoside NTPs and one of the templates. The templates used
in this assay consisted of PCNA5-luc (24) and AdML-luc (25), which have
previously been described, and CMV-luc (provided by S. Rhode), which
contains CMV-IE promoter sequence 760 to +75 placed upstream of the
luciferase reporter gene. Specific RNA transcripts were detected using
a 32P-labeled primer complementary to nucleotides +55 to
+82 of the luciferase gene and Superscript IITM RNase H
reverse transcriptase (Life Technologies, Inc.). The resultant products
of the expected size were quantitated using a PhosphorImager. The
values for Fig. 3 were generated by setting the level of product in the
presence of the control mAb to 100% and calculating the values for
products in the presence of mAb4, Fab4, or scFv according to the level
of the control.
DNA Constructs--
The pCMV-scFv and pEF-scFv expression
vectors were generated by placing the cDNA of scFv clone C9 into
the multiple cloning sites of pCMV4 and pEF-1 (provided by Dr. R. Lewis), respectively. pCMV4 incorporates the SV40 origin and the
translational enhancer from alfalfa mosaic virus 4 in addition to the
strong CMV-IE promoter (26). pEF-1 is a derivative of the pcDNA3
vector that has the CMV-IE promoter replaced with the promoter region
from elongation factor-1, which contains no CRE sequences (27).
BglII and MluI ends were introduced into scFv by
polymerase chain reaction for insertion into the respective sites of
pCMV4. The undigested scFv polymerase chain reaction product was
ligated into the Invitrogen T/A cloning vector, and the
EcoRI-NotI fragment from this ligation was
inserted into the appropriate sites of pEF-1.
Transient Cotransfections--
Transient cotransfections of HeLa
cells were performed according to established protocols using calcium
phosphate precipitation (28). The transfections were performed three to
four times in duplicate 35-mm wells containing one of the reporter
constructs (CMV-luc (5 µg), PCNA-luc (10 µg), and AdML-luc (10 µg)) and increasing amounts of the scFv vectors (0, 5, 10, and 20 µg). To control the assays for variations in transfection efficiency,
a Rous sarcoma virus--galactosidase construct (2 µg) was used as
an internal standard. Additionally, in the wells without scFv, a molar
equivalent of parent vector was used (without cDNA insert) to
maintain an equal number of promoter units in each transfection. The
cells were harvested 60-72 h post-transfection, and the reporters were assayed.
Luciferase and -Galactosidase Assays--
Measurement
of the reporter activity of firefly luciferase was carried out as
described relative to an internal
-galactosidase standard (29).
60-72 h post-transfection, cell extracts were prepared by freeze-thaw
lysis in a potassium phosphate buffer. ATP and luciferin were added,
and light output was measured with a Luminoskan RS microplate
luminometer (Lab Systems/Denley, Franklin, MA). To measure
-galactosidase,
o-nitrophenyl-
-D-galactopyranoside was added,
and the absorbance at 405 nm was read on an ELISA plate reader. The
luciferase value of each well was normalized to the internal
-galactosidase reporter. Results of three to four experiments were
then averaged to generate the data depicted in Fig. 4.
![]() |
RESULTS |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Sequence of scFv4--
Our goal of investigating the cellular
function of ATF1 and CREB prompted us to clone the scFv fragment of
mAb4 from its parental hybridoma cell line. However, the commonly used
myeloma cell line fusion partner, P3/NS1/1-Ag4-1 (NS1), constitutively
secretes a light chain (30) that can interfere with the cloning of scFv fragments from these hybridomas. Confirmation that the correct Fv
fragment was cloned was obtained by automated sequencing of the scFv
fragment. The amino acid sequence of the recombinant Fv fragment was
deduced from the nucleotide sequence of cDNA and compared with the
previously obtained N-terminal sequence of the parental monoclonal
antibody light chain. The N-terminal 55 amino acids of the
VL domain of mAb4, extending from framework region 1 through CDR2, were conserved in scFv, confirming that scFv4 did not
contain the NS1
chain.
scFv4 Maintains the Immunoactivity of mAb4 in Competitive
ELISA--
Cloned scFv fragments often maintain the activity of the
parental antibody. However, a number of factors may influence the binding characteristics of the scFv fragment, including structural perturbations due to the absence of the constant regions, the linkage
order of the variable domains (5'-VL-VH-3'
versus 5'-VH-VL-3'), and the
composition and length of the linker (35, 36). The initial binding
characteristics of scFv4 as compared with mAb4 and Fab4 were evaluated
through competitive ELISAs using ATF1 as antigen (7, 8). The scFv
fragment was demonstrated to specifically bind immobilized ATF1 as
determined by competition with soluble full-length recombinant ATF1.
Additional competitive ELISAs were performed using soluble peptide C as
competitor. Peptide C is the previously defined pentadecamer epitope of
mAb4 (7) that is adjacent to the basic DNA-binding region (Fig.
1A). The ability of peptide C
to inhibit the binding of mAb4, Fab4, scFv4, and a control periplasmic
extract to immobilized ATF1 was compared (Fig. 1B). The
competition curves generated were used to determine the peptide
concentration giving 50% inhibition of binding. This value
(IC50) can be assumed to be a function of affinity
(Kd) and allows comparison between antigen binding
activities (37). The IC50 of soluble peptide C for scFv
binding to immobilized ATF1 was determined to be 1.5 × 107 M. This is in the same range as the
IC50 values for mAb4 and Fab4, which are 1 × 10
7 and 2 × 10
7 M,
respectively. A control peptide (peptide X) of similar length and
charge to peptide C did not inhibit the binding of scFv4 by immobilized
ATF1 (Fig. 1B).
|
scFv4 Specifically Inhibits ATF1 and CREB Binding to the CRE
in EMSAs--
We have previously shown that both mAb4 and Fab4 inhibit
ATF1 binding to a CRE in gel mobility shift assays (7, 8). The
inhibitory effect of scFv4 on ATF1·CRE complex formation was equal to
that which occurred with mAb4 or Fab4 (Fig.
2A, left panel). When molar equivalents of scFv, mAb4, or Fab4
(normalized for valence) were used, the Fv fragment inhibited
ATF1·CRE complex formation by 95%. Although mAb4 did not inhibit
CREB·CRE complex formation in EMSA, Fab4 partially inhibited specific
complex formation (Fig. 2A, right
panel, third lane). This finding is
consistent with our previous in vitro transcription results
showing partial inhibition of the CMV-IE promoter by Fab4 since CREB is
believed to contribute to the transcriptional activity of the CMV-IE
promoter (8). A clear demonstration of the reactivity of scFv4 with CREB was apparent in the EMSA studies, where scFv4 reduced CREB·CRE complex formation to 5% of controls (Fig. 2A,
right panel, fourth lane),
whereas equivalent amounts of mAb4 had no effect, and Fab4 had a
partial effect. The inhibition of ATF1 or CREB binding occurred whether
the Fv fragment was added before or after the reactions were at
equilibrium. Additional faster migrating complexes were present that
were eliminated by addition of nonspecific competitor DNA, suggesting
they were due to proteins present in the recombinant CREB preparation.
Mutant derivatives of bZIP family proteins, CREB and
ATF2, were
used to demonstrate specific activity of scFv in EMSA studies.
CREB
was chosen because it contains the dimerization and DNA-binding domains
of CREB but is truncated at the beginning of the epitope (peptide F
region) that interacts with scFv4. EMSA studies showed no interactions
between
CREB and mAb4, Fab4, or scFv4 (Fig. 2B,
left panel). In contrast, the binding of
ATF2,
which does not share any homology in the region of epitope C or F, to a
CRE was not affected by the presence of mAb, Fab, or scFv (Fig.
2B, right panel).
|
scFv4 Prevents Transcription from CRE-driven Promoters in Vitro-- The binding of DNA by transcription factors and the process of transcriptional activation are related but separate functions. Since the scFv fragment prevents binding of ATF1 to DNA, the reduction of transcriptional activation was the anticipated secondary effect. In vitro transcription experiments were therefore important to determine whether the scFv inhibition of ATF1 and CREB binding to DNA resulted in reduced levels of transcription. Fig. 3A shows a comparison of the promoter constructs used in the in vitro assays. Our interest was to determine whether the apparent reactivity of scFv4 for ATF1 and CREB in EMSA would correlate with inhibitory activity using a promoters such as those of PCNA and CMV-IE. As a control for nonspecific interference of transcription, the AdML promoter was chosen. The AdML promoter (Fig. 3A) is a minimal promoter containing a TATA box and a pair of MAZ and Sp1 elements (38-40). There are no CREs or CRE-like sequences present, allowing for demonstration of specificity of the mAb and scFv. scFv4 was found to inhibit transcription from the PCNA promoter by 93% (Fig. 3B), comparable to the level of inhibition achieved previously with the mAb and Fab fragment (7, 8). Our previous experiments demonstrated that Fab4 reduced the production of product expressed from the CMV-IE promoter in vitro by 95% (8). We observed that incremental increases of scFv added to transcription reactions progressively decreased the intensity of transcript product to approximately two-thirds of that with Fab4. No effect was observed on the production of transcription product from the AdML promoter in the presence of Fab4, mAb4, or scFv4. The control periplasmic extract did not alter expression of the transcription products from these promoters, nor did control antibodies.
|
Intracellular Expression of scFv Interferes with CRE-driven
Transcription--
HeLa cells were chosen to determine the
intracellular effects of scFv as in vitro transcription
assays had been performed using HeLa cell nuclear extracts. ATF1 has
been shown to be expressed at significantly higher levels than CREB in
HeLa cells (4); therefore, we expected to observe significant
expression from the CRE-driven luciferase reporters. The three separate
luciferase reporters (CMV-luc, PCNA-luc, and AdML-luc) used in the
in vitro transcription assays were cotransfected with either
scFv expression vectors or control vectors without a cDNA insert.
To control the experiments for variations in transfection efficiency, a
Rous sarcoma virus--galactosidase construct was used. By assaying the
-galactosidase activity and normalizing for transfection efficiency, the cellular transcriptional activity was compared between
experiments and the different constructs. A base line for each reporter
was thereby established. The strong CMV-IE-luc reporter produced ~50
relative light units/µl of lysate, whereas the weaker PCNA-luc and
AdML-luc reporters generated 0.3 and 0.02 relative light units/µl,
respectively.
|
![]() |
DISCUSSION |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Although studied extensively, the cellular roles of ATF1 and CREB have not been fully determined. CREB is an important mediator of cAMP-activated transcription, whereas ATF1 activates transcription in some cell lines (41) and represses transcription in others (3). To elucidate the roles of transcription factors, investigators have attempted to knock out the activity of the transcription factors. In studies with ATF1 and CREB, activity has been reduced in vitro with an ATF1-sequestering CRE oligonucleotide (42) and in vivo through the generation and study of transgenic and mutant mice (6, 43, 44). Other investigators have used intracellularly expressed scFv fragments as modified gene knockout systems (12, 13). The recent success in the application of these modified knockout systems led us to evaluate the inhibitory activity of the scFv fragment derived from mAb4.
The ATF1 binding activity of mAb4 was maintained by scFv in ELISA (displaying a similar affinity for ATF1). In EMSA, scFv inhibited the formation of ATF1·CRE complexes, as had occurred with the mAb, and also inhibited the formation of CREB·CRE complexes, similar to the effect of the Fab fragment. Previous studies in our laboratory demonstrated that both mAb4 and Fab4 inhibited transcript production from the PCNA promoter (7, 8). The inhibition of ATF1·CRE and CREB·CRE complexes in EMSA correlated with reduction of expression from the CRE-containing promoters in in vitro transcription assays. The cell transfection experiments confirmed the inhibitory effect of scFv on CRE-containing promoters. Therefore, the EMSA and in vitro transcription studies were predictive of the effect of scFv in cells, but the specific level of inhibition was different in each assay.
The mAb interfered solely with ATF1, whereas the Fab fragment possessed additional reactivity for CREB as observed by EMSA and in vitro transcription from the CMV-IE promoter. The scFv fragment maintained reactivity for CREB, but the affinity was lower for CREB than for ATF1. Although the scFv fragment has an apparent lower affinity for CREB than for ATF1, it has greater inhibitory activity than either the Fab fragment or mAb in vitro and maintains this activity in cells. The apparent difference in the relative reactivities of the scFv construct and its parental mAb for the two transcription factors could derive, in principle, from several factors that are still to be determined. First, structural constraints in the antigen-binding site may be imposed by the covalent linkage of the VL and VH domains via the 15-residue linker (Gly4-Ser)3) or by the order of the two domains (the VL C terminus linked to the VH N terminus versus the VH C terminus linked to the VL N terminus as in this study). Although scFv constructs generally retain the activity of the parental antibodies, the length and composition of the linker (35, 45), as well as the assembly order of the VL and VH domains, have been reported to influence the antigen binding affinity and avidity in certain cases (36, 45). Second, an increased ability of scFv to diffuse into small spaces, due to the smaller size of the former molecule (27 kDa versus 150 kDa for the mAb), may increase the accessibility of the antigenic epitope within the transcription factor-DNA complex. The tertiary structure of CREB may be such that certain domains are accessible by the small scFv fragment, but not by the large intact antibody. Third, aggregation reactions are known to occur in certain Fv preparations (46, 47), which may alter the avidity of the binding for the two transcription factors. Significant intermolecular noncovalent interactions between the variable domains present in different scFv molecules could potentially occur at the scFv concentrations employed in our experiments (0.3-3 µM), depending on the affinity constant for noncovalent VL·VH complexations. Fourth, the presence of unusual residues in our scFv fragment may indicate that non-somatic mutations are responsible for the additional activity. The scFv fragment is notable for the lack of a CDR3 in its VH domain. The CDR3 deletion in our scFv fragment most likely arose from a somatic event since nucleotide sequencing of multiple isolated clones indicated the occurrence of this deletion, and examination of the sequence data revealed a frameshift occurring at this point in the VH domain. In consideration of the above issues, we propose that scFv binds to ATF1 or CREB and limits transcription factor interaction with the CRE. Interference with the binding of ATF1 or CREB to the CRE is believed to prevent the recruitment of transcriptional machinery and subsequent gene expression. Although the reactivity of scFv for both ATF1 and CREB is a useful property, further affinity maturation toward either transcription factor may allow specific inhibition of biological activity.
Multiple approaches were taken to demonstrate that the activity of scFv was specific for the CRE-binding proteins, ATF1 and CREB. The adenovirus major late promoter was chosen because it contains only a TATA box and a pair of MAZ and Sp1 elements (38-40). No CRE or CRE-like sequences are present in the AdML promoter. Additionally, adding back ATF1 or CREB to the intracellular scFv system reversed the effects of scFv upon the PCNA promoter.
In our transfection studies, scFv showed a greater inhibitory
effect on the PCNA promoter than the CMV-IE promoter, with 56 and 25%
decreases in the promoter activities, respectively. The PCNA construct
encompasses 182 to +143 of the promoter and contains single Ap2, Oct,
and Sp1 elements as well as a tandem CRE. These two CREs have been
shown to contribute the major activity of this promoter and are
primarily activated by ATF1 (24). The CMV promoter we used contained
region
760 to +75 of the major IE promoter, and five 19-base pair
repeat elements with a CRE at each of their cores are located in this
region. In addition to CREs, the CMV-IE promoter contains other binding
sites for transcription factors, including NF-
B, Ap1, Sp1, C/EBP,
and NF1. The presence of these additional binding sites for other
transcriptional activators most likely accounts for the decreased
effect of scFv upon the CMV-IE promoter as compared with the PCNA
promoter. The relative strengths of ATF1 and CREB as activators of the
CRE (48) and the intracellular ratios of ATF1 to CREB may also
contribute to the observed effects. Whether the difference in
activation will correlate with a physiologic change in cell viability
or activity is yet to be determined. However, due to its ability to
interfere with the activity of ATF1 and CREB, the scFv fragment is a
useful tool for novel studies of CRE-mediated cellular differentiation, tumorigenesis, and viral pathogenesis.
![]() |
ACKNOWLEDGEMENT |
---|
We are grateful to Andrew H. Henry for sequence analysis of ScFv.
![]() |
FOOTNOTES |
---|
* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF039853 and AF039854.
§ Present address: Information Services Div., Harris Corp., Bellevue, NE 68005.
¶ Present address: Creighton University School of Dentistry, 2802 Webster Plaza, Omaha, NE 68131.
To whom correspondence should be addressed: Dept. of Pathology
and Microbiology, University of Nebraska Medical Center, 600 South 42nd
St., Omaha, NE 68198-6495. Tel.: 402-559-4116; Fax: 402-559-4077;
E-mail: shinrich{at}mail.unmc.edu.
1 The abbreviations used are: ATF1, activating transcription factor 1; CREB, cAMP-responsive element-binding protein; CRE, cAMP-responsive element; CREM, cAMP-responsive element modulator; mAb, monoclonal antibody; Ab, antibody; scFv, single chain Fv; VH, variable heavy; VL, variable light; CDR, complementarity-determining region; ELISA, enzyme-linked immunosorbent assay; EMSA, electrophoretic mobilityshift assay; PCNA, proliferating cell nuclear antigen; AdML, adenovirus major late; CMV-IE, cytomegalovirus immediate-early; bZIP, basic leucine zipper.
![]() |
REFERENCES |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|