Expression of Nitric-oxide Synthase in Rat Kupffer Cells Is Regulated by cAMP*

Shamimunisa B. Mustafa and Merle S. OlsonDagger

From the Department of Biochemistry, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78284

    ABSTRACT
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Abstract
Introduction
Procedures
Results
Discussion
References

Treatment of cultured rat Kupffer cells with lipopolysaccharide (LPS) resulted in a time-dependent increase in the expression of the inducible isoform of nitric-oxide synthase (iNOS). Agents that elevated intracellular cAMP levels (e.g. forskolin, dibutyryl cAMP, cholera toxin, and isoproterenol) markedly decreased nitrite production and iNOS protein formation by LPS-stimulated Kupffer cells. Furthermore, inhibition of LPS-induced nitrite formation and iNOS protein levels by these agents was enhanced in the presence of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine. Forskolin, the most potent inhibitor of LPS-induced nitrite formation by Kupffer cells, decreased iNOS mRNA levels in a time-dependent manner. Time course studies indicated that forskolin was most effective at inhibiting LPS-induced nitrite formation and iNOS mRNA levels by Kupffer cells when added before LPS. Message stability studies established that forskolin did not enhance the rate of decay of LPS-induced iNOS mRNA. Nuclear run-on assays revealed that forskolin decreased LPS-induced transcription of the iNOS gene. Treatment of Kupffer cells with LPS induced the translocation of the p65 subunit of nuclear factor kappa B (NF-kappa B) into the nucleus, and this process was abolished by forskolin. In addition, the LPS-dependent degradation of Ikappa Balpha was not observed in forskolin-treated cells; the levels of the p65 subunit of NF-kappa B were minimal in the nucleus at the same time. Also, we observed that forskolin induced transcription of the Ikappa Balpha gene in a time-dependent manner and in addition up-regulated LPS-induced Ikappa Balpha mRNA levels. Taken together, this study indicates that the attenuation of LPS-induced iNOS formation in Kupffer cells by elevated intracellular cAMP levels occurs by preventing the degradation of Ikappa Balpha which suppresses the activation of NF-kappa B and inhibits the onset of transcription of the iNOS gene.

    INTRODUCTION
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Abstract
Introduction
Procedures
Results
Discussion
References

Nitric oxide (NO)1 has been identified as an important signaling molecule that is involved in regulating a wide array of biological activities in neural, vascular, and immune cellular systems (1). NO is generated from L-arginine and molecular oxygen in the presence of the enzyme NO synthase (NOS) (2). To date three distinct NOS isoforms have been identified from molecular cloning and sequencing analyses (3). The endothelial (eNOS) and neuronal (nNOS) isoforms are expressed constitutively in endothelial and neuronal cells, respectively. The amount of NO generated by these cell types is dependent upon the cellular content of NOS (4). NO generated by endothelial cells plays an important role in the control of vascular tone, whereas in neuronal tissue NO acts to regulate cGMP-mediated neurotransmission (1, 2). The third isoform of NOS, termed inducible NOS (iNOS), was first identified in macrophages stimulated with interferon-gamma and bacterial lipopolysaccharide (LPS) (3). iNOS has been identified in a wide variety of cell types including macrophages, mesangial cells, vascular smooth muscle cells, keratinocytes, chondrocytes, osteoclasts, and hepatocytes (1, 5). NO generated within these cells mediates macrophage cytotoxicity during host defense reactions, alterations in the contractile responses of mesangial cells, and in instances where NO exceeds normal physiological levels, instigates the inhibition of vascular smooth muscle tone, hepatocyte metabolism, and protein synthesis (6-8). Recent evidence indicates that elevated levels of NO play a major role in the pathogenesis of several chronic disorders and inflammatory processes. In particular, studies have indicated that an overproduction of NO in response to LPS and cytokines contributes to the development and prolongation of severe hypotension and peripheral vasodilation observed during endotoxic shock (9).

The activities of nNOS and eNOS are regulated by rapid, transient elevations of intracellular free calcium which enhance the binding of calmodulin to the NOS enzyme resulting in NO release over a time frame of seconds and minutes (10). In contrast, the expression of iNOS is thought to be regulated primarily at the transcriptional level of the iNOS gene. Once induced, iNOS produces NO for periods of several hours or days. Thus, given the magnitude of the wide variety of inhibitory actions of NO, it is of considerable interest and even may be of some therapeutic utility to delineate the mechanism(s) by which the production and resultant activity of NOS can be controlled or attenuated.

In the presence of LPS, Kupffer cells, the resident macrophage found in the sinusoids of the liver, produce large amounts of nitrite and nitrate, the stable end products of the NO pathway (11). It has become apparent recently that overproduction of NO by hepatic cells plays a major role in hepatic injury/necrosis associated with endotoxic shock. Kupffer cells also synthesize and release several cytokines in response to LPS which in turn stimulate neighboring hepatocytes to generate NO (8). The consequent overproduction of NO in the liver results in profound degenerative changes observed in hepatocytes (12). These changes include a decrease in total protein synthesis, cellular proliferation, and an increase in cGMP formation (13). The induction of iNOS by LPS in Kupffer cells requires the initiation of gene expression and de novo protein synthesis over a period of several hours. It is unclear whether classical second messengers such as cAMP are involved in iNOS gene expression and NO formation. Recent studies have indicated that agents that elevate levels of cAMP improve circulatory function in animal models of endotoxic shock; in particular isoproterenol was found to inhibit the development of vascular hyporeactivity in the endotoxic rat (14, 15). In contrast, certain in vitro studies have shown that elevation of cAMP caused an induction of iNOS, whereas in other studies increased levels of cAMP caused a reduction in iNOS (6, 7, 16). The present study was designed to investigate whether iNOS gene expression and/or enzymatic activity is regulated by elevated levels of cAMP in cultured rat Kupffer cells.

    EXPERIMENTAL PROCEDURES
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Abstract
Introduction
Procedures
Results
Discussion
References

Isolation and Primary Culture of Rat Kupffer Cells-- Following enzymatic digestion of the rat liver, Kupffer cells were isolated by centrifugal elutriation as described previously (17). The viability of the Kupffer cell preparation was greater than 95% as determined by trypan blue exclusion. Freshly isolated Kupffer cells were maintained at 37 °C in RPMI 1640 culture medium (Life Technologies, Inc.) supplemented with 25 mM HEPES, L-glutamine, and 10% fetal bovine serum (Hyclone Laboratories, Inc., Logan, UT), 112 units/ml penicillin, and 112 units/ml streptomycin in 24-well plates or 60-mm tissue culture dishes. All cells were incubated in an atmosphere of 90% air and 10% CO2. On the 2nd day of culture the RPMI medium was changed. For experimental purposes, Kupffer cells were used within 3-4 days of their establishment in culture.

Measurement of Nitrite Formation-- Production of NO by iNOS was quantified by measuring the accumulation of nitrite in the culture medium using the Griess reaction (18). Kupffer cells were cultured in 24-well plastic tissue culture plates at a density of 1 × 106 cells/ml (1 ml/well) at 37 °C. After 4 days in culture the cells were washed, and complete medium without phenol red was added to each well. The cells were then exposed to several cAMP-elevating agents. After a specified incubation interval the medium from each well was removed. The nitrate in each sample was reduced to nitrite using the method described by Grisham et al. (19). Samples were then mixed with an equal volume of the Griess reagent (1% sulfanilamide, 5% H3PO4, 0.1% naphthylethylenediamine dihydrochloride) and incubated at room temperature for 10 min. The absorbance of each sample was measured spectrophotometrically at 543 nm using sodium nitrite as a standard. Nitrite formation was expressed as nmol/106 cells. Values denote the mean ± S.D. of quadruplicate determinations from at least two separate experiments, unless otherwise stated.

Preparation of Whole Cell, Nuclear, and Cytoplasmic Extracts-- Kupffer cells were plated at a density of 1 × 107 cells/60-mm dish. After 3 days in culture, the cells were rinsed with fresh medium and stimulated with LPS alone or with cAMP-elevating compounds. Following treatment, the cells were rinsed with phosphate-buffered saline three times; then 500 µl of lysis buffer (50 mM Tris-HCl, pH 7.4) containing 5 mM EDTA, 5 mM EGTA, 1 µM leupeptin, 1 µM pepstatin A, 1 µM aprotinin, and 1 µM phenylmethylsulfonyl fluoride was added to each dish, and the cells were scraped quickly. The resulting cell suspensions were subjected to three rapid freeze-thaw-vortex cycles to disrupt the Kupffer cells completely. A 100-µl sample of the broken cell preparation was used for protein quantitation, and the remainder of the sample was stored at -80 °C until analyzed.

Nuclear and cytoplasmic extracts were prepared according to Schreiber et al. (20). Briefly, 5 × 106 Kupffer cells were washed with cold phosphate-buffered saline and suspended in 400 µl of lysis buffer (10 mM HEPES, pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.1 M EGTA, 1 mM dithiothreitol), 0.5 mM phenylmethylsulfonyl fluoride, 1 µM leupeptin, 1 µM pepstatin A, and 1 µM aprotinin. The cells were allowed to swell on ice for 15 min, after which 12.5 µl of 10% Nonidet P-40 was added. The tube was vortexed vigorously for 10 s, and the homogenate was centrifuged for 30 s in a microcentrifuge. The supernatant (cytoplasmic extract) was removed carefully and stored at -80 °C until required. The nuclear pellet was resuspended in 25 µl of cold nuclear extraction buffer (20 mM HEPES, pH 7.9, 0.4 M NaCl, 1 mM EDTA, 1 mM EGTA, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, 1 µM leupeptin, 1 µM pepstatin A, and 1 µM aprotinin), and the tube was incubated on ice for 30 min with frequent vortexing. The nuclear extract was then centrifuged for 5 min in a microcentrifuge at 4 °C, and the supernatant was stored at -80 °C for later use.

Western Blot Analysis for iNOS-- Before SDS-PAGE the disrupted cell suspensions were dried using a Savant vacuum centrifuge. Sample pellets were solubilized in buffer containing 0.1 M dithiothreitol, 50% glycerol, 0.5 M Tris, pH 6.8, 2.5 mM pyronine Y, and 20% SDS and subjected to SDS-PAGE (7.5% gel) using the buffer system of Laemmli (21). The separated proteins were transferred electrophoretically to polyvinylidene difluoride membranes, using a semidry transfer blot system, and the membranes were soaked for 1 h in Tris-buffered saline, pH 7.4, containing 5% non-fat dried milk powder and incubated for 24 h with anti-iNOS antibody in 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.01% Tween 20, and 1% bovine serum albumin. The blots were then incubated with horseradish peroxidase-labeled goat anti-mouse IgG in the same buffer for 2 h. Finally, the blots were rinsed in 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, and 0.01% Tween 20. Peroxidase-labeled proteins were visualized by incubation with the peroxidase color development reagent 3,3'-diaminobenzidine and hydrogen peroxide.

Western Blot Analysis for p65 and Ikappa Balpha -- Cytoplasmic and nuclear samples were resolved using SDS-PAGE (11% gel). The separated proteins were electrotransferred to polyvinylidene difluoride membranes. The membranes were then incubated in blocking buffer (Tris-buffered saline, pH 7.4) containing 10% non-fat dried milk powder for 1 h and then exposed to diluted primary antibodies against the p65 subunit of NF-kappa B or Ikappa Balpha overnight at 4 °C. The membranes were incubated for 1 h at room temperature with 5,000-fold diluted horseradish peroxidase-conjugated anti-rabbit IgG antibody. Protein bands were visualized using an enhanced chemiluminescence (ECL) assay kit.

Northern Blot Analysis-- Kupffer cells were plated at a density of 1 × 107 cells/60-mm dish. After 3 days in culture and the appropriate treatment, total RNA from cultured rat Kupffer cells was isolated using TRIzol reagent (Life Technologies, Inc.). RNA (3-4 µg) was separated by electrophoresis on a 0.8% agarose, 2.2 M formaldehyde gel and transferred using a Possiblot (Stratagene, La Jolla, CA) onto a Magna nylon membrane (Microns Separations Inc. Westborough, MA). A full-length murine iNOS cDNA probe kindly provided by Dr. S. H. Snyder (The Johns Hopkins University School of Medicine, Baltimore) or Ikappa -B cDNA kindly provided by Dr. A. Baldwin Jr. (University of North Carolina, Chapel Hill) was labeled with a multiprime DNA labeling system using [alpha -32P]dCTP (specific activity, 3,000 Ci/mmol). Northern blot hybridizations were performed in 50% formamide, 1 M NaCl, 10% dextran sulfate, 50 mM Tris-HCl, pH 7.5, 0.1% sodium pyrophosphate, and 0.2% Denhardt's solution at 42 °C for 16 h. The membranes were washed twice in 2 × SSC, 1% SDS at 65 °C for 20 min, twice in 0.1 × SSC, 0.1% SDS at 55 °C for 15 min, and finally at room temperature in 0.1 × SSC for 15 min. Radioactivity was visualized using a PhosphorImager (Molecular Dynamics, Sunnyvale, CA). Control hybridizations were performed using a 32P end-labeled oligonucleotide complementary to rat 18 S rRNA.

Nuclear Run-on Analysis-- Nuclei were isolated from treated Kupffer cells (5-6 × 107) according to standard protocols (22). Briefly, cells were rinsed once in ice-cold phosphate-buffered saline, scraped gently in 6 ml of phosphate-buffered saline, and centrifuged at 800 × g at 4 °C for 5 min. Lysis buffer (10 mM Tris-Cl, 10 mM NaCl, 3 mM MgCl2, 0.5% (v/v) Nonidet P-40, 2 ml) was added followed by vortexing for 10 s to disrupt the cell pellets. The cells were incubated on ice for 5 min and nuclei pelleted by centrifugation at 800 × g at 4 °C for 5 min. The pelleted nuclei were resuspended in storage buffer (50 mM Tris-HCl, 5 mM MgCl2, 0.1 mM EDTA, 40% (v/v) glycerol), frozen, and then stored in liquid nitrogen until needed. For in vitro transcription, freshly thawed nuclei were incubated in reaction buffer (10 mM Tris-HCl; 5 mM MgCl2; 0.3 M KCl; 100 mM ATP, CTP, GTP; 1 M dithiothreitol; 10 mCi/ml [alpha -32P]UTP (3,000 mCi/mmol, Amersham Corp.), shaking for 30 min at 30 °C. The reaction was quenched by the addition of RNase-free DNase I (10 mg/ml, Worthington) and proteinase K (20 mg/ml, Ambion, Austin, TX) and incubated for 30 min at 42 °C. Nascent labeled RNAs were purified by extraction with phenol/chloroform and two sequential precipitations with ammonium acetate. Equal amounts of 32P-labeled RNA were resuspended in 1 ml of TES/NaCl solution (10 mM TES, pH 7.4, 10 mM EDTA, 0.2% SDS, 0.3 M NaCl) and hybridized for 60 h at 65 °C to denatured DNA probes immobilized on nitrocellulose membranes. Following hybridization, the filters were washed twice in 2 × SSC at 65 °C for 1 h, once at 37 °C in 2 × SSC containing RNase A (10 mg/ml, Ambion) for 30 min, and once in 2 × SSC at 37 °C for 1 h. Radioactivity was visualized using a PhosphorImager.

Materials-- Anti-mac iNOS was obtained from Transduction Laboratories (Lexington, KY). Rabbit polyclonal antibodies raised against p65 and Ikappa Balpha were purchased from Santa Cruz Biotech Inc. (Santa Cruz, CA). Goat anti-mouse and goat anti-rabbit IgG horseradish peroxidase conjugate and prestained SDS-PAGE standards were obtained from Bio-Rad. Bacterial LPS (from Escherichia coli, serotype 011:B4), dibutyryl cAMP, cholera toxin, forskolin, isoproterenol, IBMX, and actinomycin D were purchased from Sigma.

    RESULTS
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Abstract
Introduction
Procedures
Results
Discussion
References

cAMP-elevating Agents Inhibit LPS-induced Nitrite and iNOS Protein Formation by Kupffer Cells-- Cultured unstimulated Kupffer cells exhibited a low basal level of nitrite production, whereas LPS caused an 8-fold increase in nitrite formation during the 24-h observation period (Table I). The addition of various cAMP-elevating agents attenuated LPS-induced nitrite formation by Kupffer cells, each in a dose-dependent manner (data not shown). The effect of the highest concentration of each agent used in this study is noted in Table I. The diterpene forskolin activates adenylate cyclase directly, resulting in an increase of intracellular cAMP levels; forskolin inhibited LPS-induced nitrite formation strongly. The membrane-permeable cAMP analog dibutyryl cAMP reduced LPS-induced nitrite formation by 34%. Both isoproterenol and cholera toxin stimulate adenylate cyclase via the stimulatory G-protein Gs, isoproterenol by binding to cell surface beta -adrenergic receptors and cholera toxin by ADP-ribosylation of Gs. We have used these agents previously to demonstrate cAMP-dependent changes in platelet-activating factor binding in Kupffer cells, and we have confirmed that isoproterenol causes an increase in intracellular cAMP in these cells (23). It is important to note that none of the cAMP-elevating agents (at the indicated concentrations) caused 1) any significant change in the rate of formation of nitrite above the control value when added to Kupffer cells in the absence of LPS (Table I) and 2) any morphological alterations or detachment of Kupffer cells after an incubation period of 24 h. In addition, LPS-induced nitrite formation by Kupffer cells in the combined presence of the aforementioned cAMP-elevating agents and the phosphodiesterase inhibitor IBMX was inhibited to a greater degree than in the absence of IBMX (Table I). Thus cAMP-elevating agents clearly are capable of decreasing LPS-induced iNOS activity in Kupffer cells.

                              
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Table I
Effects of various cAMP-elevating agents on LPS-stimulated nitrite production by Kupffer cells
Kupffer cells (1 × 106) were pretreated initially with cAMP-elevating agents for 1 h. They were then treated with or without LPS (10 ng/ml) and IBMX (5 mM) for 24 h. Nitrite accumulation in the culture medium was measured as described under "Experimental Procedures."

Elevated cAMP levels might exert direct metabolic control on preexisting iNOS or might decrease the synthesis of iNOS protein. To distinguish between these alternatives, the nitrite inhibition experiments were repeated, and the levels of iNOS protein in Kupffer cells were analyzed by immunoblotting with anti-iNOS antibody. As depicted in Fig. 1, in untreated Kupffer cells immunoreactive iNOS was undetectable, whereas LPS-treated cells produced a heavily stained band. The iNOS protein bands from Kupffer cells that had been treated with LPS, cAMP-elevating agents, and IBMX appeared to be lightly stained (lanes 3-6), indicating a substantial decrease in the amount of iNOS protein formed after 24 h. As forskolin was the most effective inhibitor of LPS-induced nitrite and iNOS protein formation by cultured Kupffer cells (Table I and Fig. 1) it was used for all subsequent experiments.


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Fig. 1.   Effects of agents that elevate cellular cAMP on LPS-induced iNOS protein formation by Kupffer cells. Kupffer cells were stimulated with vehicle (lane 1), LPS (10 ng/ml, lane 2), LPS and forskolin (50 µM) and IBMX (5 mM, lane 3), LPS and dibutyryl cAMP (10 µM) and IBMX (5 mM, lane 4), LPS and cholera toxin (1 ng/ml) and IBMX (5 mM, lane 5), and LPS and isoproterenol (10 µM) and IBMX (5 mM, lane 6) for 24 h. Samples were subjected to SDS-PAGE followed by immunoblot analysis using an iNOS antibody. Results are representative of three independent experiments, which gave essentially identical results.

Forskolin Inhibited LPS-induced Nitrite Formation and iNOS mRNA Levels in a Time-dependent Manner-- Kupffer cells were treated with either LPS or LPS and forskolin; nitrite formation was measured at the time intervals indicated in Fig. 2A. In agreement with other research groups, LPS stimulated nitrite formation by Kupffer cells in a time-dependent fashion. The inhibitory effects of forskolin on LPS-induced nitrite formation by Kupffer cells became apparent after 6-8 h of treatment and continued up to 24 h. The time dependence of LPS-induced iNOS mRNA accumulation in Kupffer cells is shown in Fig. 2, B and D; the iNOS mRNA level increased rapidly between 3 and 6 h and then declined by 24 h. The addition of forskolin caused a considerable decrease in the accumulation of iNOS mRNA, and the levels of message were barely detectable at 24 h (Fig. 2, C and D).


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Fig. 2.   Time course of the effects of forskolin on LPS-induced nitrite formation and iNOS mRNA levels. Panel A, Kupffer cells were stimulated with either LPS (10 ng/ml; filled squares) or LPS and forskolin (50 µM; open squares) for various periods of time. Nitrite accumulated in the medium was measured. Panel B, Kupffer cells were stimulated with LPS (10 ng/ml) or (panel C) with LPS and forskolin (50 µM) for various periods of time. After cell lysis and RNA purification, samples were hybridized with a cDNA probe for iNOS (upper) and then with a probe for 18 S rRNA (lower). The experiment is representative of three separate experiments, which gave nearly identical results. Panel D, graph of data from panels B and C, with smoothed interpolation lines. Filled squares, LPS alone; open squares, LPS and forskolin.

Kinetics of Inhibition of LPS-induced Nitrite and iNOS mRNA Formation by Forskolin-- Forskolin was added to Kupffer cells before, at the same time as, or at different times after the addition of LPS to determine the optimum time for the inhibition of LPS-induced nitrite production and iNOS mRNA levels. Nitrite accumulation in the culture medium was measured 24 h after the addition of LPS. Fig. 3A shows that maximal suppression of LPS-induced nitrite formation by Kupffer cells occurred when forskolin was present 1 h before the addition of LPS. When forskolin was added after LPS, its inhibitory effect decreased gradually with time. Fig. 3B depicts a similar experiment except iNOS mRNA was isolated and analyzed by Northern blotting. When Kupffer cells were pretreated with forskolin for 1 h before the addition of LPS, the level of iNOS mRNA formed after 6 h was barely detectable. However, the intensities of the iNOS mRNA bands increased when forskolin was added at the same time as LPS and subsequently 2 and 4 h after the addition of LPS.


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Fig. 3.   Kinetics of inhibition of LPS-induced nitrite and iNOS mRNA formation by forskolin. Panel A, Kupffer cells were incubated with LPS (10 ng/ml). Forskolin (50 µM) was added to cultures simultaneously with LPS or delayed for different time intervals. Nitrite accumulated in the medium was measured 24 h after the addition of LPS. Panel B, Kupffer cells were untreated (lane 1) or treated with LPS (10 ng/ml, lanes 2-6). Forskolin (50 µM) was added 1 h before LPS (lane 3) at the same time as LPS (lane 4) and 2 and 4 h after LPS (lanes 5 and 6, respectively). After cell lysis and RNA purification 6 h after the addition of LPS, samples were hybridized with a cDNA probe for iNOS (upper) and then with a probe for 18 S rRNA (lower). Data shown are representative of two separate experiments, which gave essentially identical results.

Effects of LPS and Forskolin on the Half-life of iNOS mRNA-- To examine whether forskolin attenuated LPS-induced steady-state levels of iNOS mRNA by decreasing its stability, we assessed the effects of forskolin on the half-life of LPS-induced iNOS mRNA by coincubation of Kupffer cells with the transcriptional inhibitor actinomycin D. Kupffer cells were treated with LPS in the presence and absence of forskolin for 6 h to induce maximal iNOS mRNA accumulation. Actinomycin D (10 ng/ml) was added to the cells at this point to inhibit further transcription. At different times after the addition of actinomycin D, total RNA was isolated and examined by Northern analysis. To allow for differences in loading, the signal density of each RNA sample hybridized to the iNOS probe was corrected by that hybridized to the 18 S probe. Fig. 4 shows the decay of iNOS mRNA as ln (relative intensity) against time. Under these conditions, the half-life of iNOS mRNA can be calculated as -(ln (2)/gradient of regression line). The calculated half-lives of iNOS mRNA in LPS-stimulated Kupffer cells in the absence and presence of forskolin were 2.4 and 2.3 h, respectively; therefore, the reduction of LPS-induced iNOS mRNA levels by forskolin in Kupffer cells was not caused by a decrease in message stability.


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Fig. 4.   Effects of LPS and forskolin on the half-life of iNOS mRNA. Kupffer cells were treated with LPS (10 ng/ml, filled squares) and with LPS and forskolin (50 µM, open squares) for 6 h before the addition of actinomycin D (10 ng/ml). At the indicated times cells were lysed and RNA purified. RNA samples were hybridized with a cDNA probe for iNOS and then with a probe for 18 S rRNA. The 18 S signal was used to correct the iNOS signal for differences in loading of each RNA sample. The data are means of three independent experiments. Regression coefficients were 0.97 and 0.95 for LPS alone and LPS plus forskolin, respectively.

Effect of Forskolin on Transcription of the iNOS Gene-- iNOS gene transcription in Kupffer cells was measured directly using a nuclear run-on assay to confirm that forskolin caused inhibition of this process. Kupffer cells were incubated either alone, or with LPS or LPS and forskolin for 3 h and 5 h; cells were lysed and nuclei isolated. The transcription of iNOS and beta -actin by isolated nuclei was determined by hybridizing the elongated, labeled RNA transcripts to iNOS- and beta -actin-specific cDNA fragments that had been slot blotted onto a nitrocellulose membrane. Fig. 5A shows that iNOS gene transcription was barely detectable in control cells (lane 1), was increased greatly by 3-h exposure to LPS (lane 2), and that pretreatment with forskolin for 1 h attenuated the LPS effect (lane 3). In a similar experiment using a 5-h stimulation with LPS, a 1-h pretreatment with forskolin caused no apparent attenuation of iNOS gene transcription (compare lanes 2 and 3 in Fig. 5B). These results are in agreement with time-dependent changes in iNOS mRNA. Fig. 2D shows that after a 3-h stimulation with LPS a pulse of iNOS gene transcription has just commenced, and the effect of added forskolin will be maximal, i.e. the gradients of the curves with and without forskolin differ considerably. After 5 h of LPS stimulation the pulse of iNOS gene transcription is ending; mRNA levels fall after 6 h. At this time the effect of added forskolin will be minimal; the gradients of the curves with and without forskolin will differ very little as both approach their highest values. Taken together, the above findings indicated that forskolin attenuates LPS-induced iNOS mRNA formation, mainly at the transcriptional level.


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Fig. 5.   iNOS gene transcription in LPS- and forskolin-stimulated Kupffer cells. Kupffer cells were unstimulated (lane 1) or stimulated with 10 ng/ml LPS alone (lane 2) or in combination with 50 µM forskolin (lane 3) for 3 h (panel A) and 5 h (panel B). The transcription of iNOS and beta -actin by isolated nuclei was determined by hybridizing the labeled RNA transcripts to NOS and beta -actin cDNA. The results are representative of two independent experiments.

Forskolin Decreased the Translocation of the p65 Subunit of NF-kappa B from the Cytoplasm into the Nucleus-- Recent studies by Xie et al. (24) have shown that activation of the transcription factor NF-kappa B and its binding to the promoter region of the iNOS gene are critical steps in the induction of iNOS synthesis by LPS in macrophages. In addition, Tran-Thi et al. (25) reported NF-kappa B-binding activity in Kupffer cells treated previously with LPS. In view of these findings, we decided to investigate the effect of forskolin on NF-kappa B activation in the presence and absence of LPS. Kupffer cells were either untreated, treated with LPS for 30 min, pretreated with forskolin for 1 h and then stimulated with LPS for 30 min, or pretreated with forskolin for 1 h and then stimulated with vehicle for 30 min; cytoplasmic and nuclear proteins were then isolated. A representative experiment is depicted in Fig. 6. Panel A represents cytoplasmic extracts, and panel B represents nuclear extracts. In untreated cells (lane 1) the p65 subunit of NF-kappa B was located mainly in the cytoplasm. A residual amount of p65 was detected in the nuclear extract. In contrast, LPS (lane 2) increased greatly the amount of p65 detected in the nucleus of Kupffer cells. Interestingly, in forskolin-treated Kupffer cells, both in the presence (lane 3) and absence (lane 4) of LPS, the amount of p65 detected in the nuclear extract was greatly diminished compared with LPS-treated cells (lane 2). Practically all of the p65 was retained in the cytoplasm (lanes 3 and 4), suggesting that forskolin interfered with the translocation of p65 from the cytoplasm into the nucleus.


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Fig. 6.   Effect of forskolin on the level of the p65 subunit of NF-kappa B. Kupffer cells were untreated (lane 1), stimulated with LPS (10 ng/ml) for 30 min (lane 2), pretreated with forskolin (50 µM) for 1 h and then incubated with LPS (10 ng/ml) for 30 min (lane 3), or treated with forskolin (50 µM) for 1 h (lane 4). Cytoplasmic protein (3 µg, panel A) and nuclear protein (3 µg, panel B) samples were subjected to SDS-PAGE followed by immunoblot analysis using a p65 antibody. Data shown are representative of two separate experiments, which gave essentially identical results.

Effects of Forskolin on the LPS-induced Degradation of Ikappa Balpha -- NF-kappa B proteins reside in the cytoplasm of resting cells complexed to a family of inhibitory proteins designated Ikappa B, which includes Ikappa Balpha and Ikappa Bbeta subunits. Activation of NF-kappa B, which results in the translocation of the free protein into the nucleus, is preceded by a rapid phosphorylation and proteolytic degradation of Ikappa B subunits (26). Using Western blot analysis we examined the levels of Ikappa Balpha protein, a major form of Ikappa B in the cytoplasmic extracts of untreated and treated Kupffer cells. A representative experiment is depicted in Fig. 7. In cytoplasmic extracts from untreated Kupffer cells (lane 1), an Ikappa Balpha -specific antibody detected an intense single band with a molecular mass of about 45 kDa. In contrast, after 30 min of treatment of Kupffer cells with LPS (lane 2), the amount of Ikappa Balpha protein detected was reduced greatly compared with that in untreated cells. However, in forskolin-treated Kupffer cells both in the presence (lane 3) and absence (lane 4) of LPS, the levels of Ikappa Balpha detected in the cytoplasm were comparable to the levels of the protein formed in untreated cells (compare lanes 1, 3, and 4). This finding strongly indicated that in the presence of forskolin the Ikappa Balpha protein in the cytoplasm of Kupffer cells remains intact and remains complexed to the NF-kappa B proteins.


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Fig. 7.   Effect of forskolin on the degradation of Ikappa Balpha in Kupffer cells. Kupffer cells were untreated (lane 1), stimulated with LPS (10 ng/ml) for 30 min (lane 2), incubated with forskolin (50 µM) for 1 h and then treated with LPS (10 ng/ml) for 30 min (lane 3), and incubated with forskolin (50 µM) for 1 h (lane 4). Cytoplasmic extracts were isolated as explained earlier. Western blotting analysis was performed using an antibody against Ikappa Balpha . Data shown are representative of two separate experiments, which gave essentially identical results.

Effect of Forskolin and LPS on Ikappa Balpha mRNA Levels in Kupffer Cells-- The above results showed that in the presence of forskolin, the integrity of Ikappa Balpha protein in the cytoplasm of Kupffer cells was maintained. We then investigated whether forskolin actually initiated Ikappa Balpha gene expression in Kupffer cells. Kupffer cells were stimulated with either forskolin or LPS or LPS and forskolin and at the times indicated in Fig. 8. RNA was isolated and employed in Northern blot analyses. The mRNA for Ikappa Balpha in Kupffer cells appeared as a single band at approximately 1.6 kilobases. It is important to note here that in untreated Kupffer cells the level of Ikappa Balpha mRNA was undetectable (data not shown). In the presence of forskolin (Fig. 8, panels A and D), Ikappa Balpha mRNA levels peaked after 2 h of stimulation and then declined to negligible levels by 6 h. In contrast, in the presence of LPS (panels B and D), Ikappa Balpha mRNA levels increased just after 30 min of stimulation, reached a peak by 1 h, and then remained elevated for up to 6 h. When Kupffer cells were pretreated for 1 h with forskolin and then stimulated with LPS over a period of 6 h (panels C and D), it was observed that forskolin up-regulated LPS-induced Ikappa Balpha mRNA levels for up to 4 h after the addition of LPS (Fig. 8D). These findings suggest that forskolin has the capacity to induce Ikappa Balpha mRNA synthesis and in turn increase the levels of protein in the cytoplasm of Kupffer cells. In the event of pretreating Kupffer cells with forskolin and subsequent stimulation by LPS the cytoplasmic levels of Ikappa Balpha are maintained and thereby attenuate the translocation of NF-kappa B into the nucleus.


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Fig. 8.   Kinetics of forskolin- and LPS-induced Ikappa Balpha mRNA levels. Kupffer cells were incubated with forskolin (50 µM, panel A) or LPS (10 ng/ml, panel B) or pretreated with forskolin (50 µM) for 1 h and then incubated with LPS (10 ng/ml, panel C) for various periods of time. After cell lysis and RNA purification, samples were hybridized with a cDNA probe for Ikappa Balpha (upper) and then with a probe for 18 S rRNA (lower). Panel D, to correct for differences in loading the signal density of each RNA sample hybridized to the Ikappa Balpha probe was divided by that hybridized to the 18 S probe. The data are expressed as means ± S.D. of three separate experiments. Filled squares, LPS; open squares, LPS plus forskolin; filled circles, forskolin alone.

    DISCUSSION
Top
Abstract
Introduction
Procedures
Results
Discussion
References

The release of NO by Kupffer cells under conditions of endotoxic shock represents an important contribution to the pathophysiology of the liver (27). Thus it is of considerable interest to investigate the intracellular signaling pathways that regulate the induction/suppression of iNOS gene expression. In Kupffer cells, agents that elevate intracellular cAMP levels attenuated both LPS-induced nitrite formation (Table I) and LPS-induced iNOS protein (Fig. 1). Furthermore, in the presence of forskolin LPS-induced iNOS mRNA formation was suppressed greatly (Fig. 2, C and D). This suggested that elevated levels of cAMP may have 1) directly suppressed the onset of iNOS gene transcription, 2) decreased transcription of the iNOS gene, or 3) destabilized iNOS mRNA following transcription. The half-life of iNOS mRNA was essentially the same in the presence or absence of forskolin, eliminating the possibility of altered mRNA stability (Fig. 4). Experiments in which forskolin was added to cultured Kupffer cells before, with, or at different times after LPS indicated that maximal inhibition of iNOS mRNA required the presence of forskolin before the addition of LPS (Fig. 3B). This favors alternative 1) above, i.e. suppression of the initiation of transcription. Direct measurement of iNOS gene transcription showed that at 3 h after LPS stimulation forskolin decreased (Fig. 5A) the transcriptional process substantially, whereas by 5 h after LPS stimulation forskolin had little effect.

The promoter region of the recently cloned rat gene encoding iNOS has been found to contain consensus sequences for the binding of numerous transcription factors (28). Activation of these factors is critical in the induction of iNOS by LPS or cytokines. One transcription factor of paramount importance required during the induction of iNOS by LPS in macrophages is NF-kappa B. The promoter region of the rat gene encoding iNOS contains two copies of the NF-kappa B binding site consensus sequence (24). NF-kappa B is an inducible, ubiquitous transcription factor present in the cytoplasm of cells. It is composed of a dimer of p50 and p65 (Rel A) subunits. In resting cells the NF-kappa B complexes are sequestered in the cytoplasm by association with a family of inhibitory proteins which includes mainly Ikappa Balpha and Ikappa Bbeta (26). Activation of NF-kappa B can be initiated by a variety of agents including mitogens such as phorbol 12-myristate 13-acetate and inflammatory cytokines such as LPS and tumor necrosis factor alpha . After cellular activation the Ikappa Balpha proteins undergo phosphorylation and subsequent proteolytic degradation via the ubiquitin pathway (29). Free NF-kappa B protein rapidly translocates into the nucleus and binds to its consensus DNA sequence(s) (26) regulating a variety of genes responsible for immunological and inflammatory reactions (30).

As noted above, pretreatment of Kupffer cells by forskolin resulted in maximal attenuation of LPS-induced iNOS mRNA in Kupffer cells. We surmised that forskolin inhibited the LPS-stimulated nuclear translocation of NF-kappa B in Kupffer cells, resulting in decreased transcription of the iNOS gene and consequently decreased steady-state levels of iNOS mRNA. Western blot analysis of cytoplasmic and nuclear extracts (Fig. 6) confirmed that in Kupffer cells forskolin pretreatment greatly reduced the amount of NF-kappa B (p65 subunit) which was translocated to the nucleus by LPS stimulation. A similar result was obtained using cytoplasmic and nuclear extracts from Kupffer cells that had been treated only with forskolin. These findings confirmed our conjecture that forskolin inhibits LPS-induced iNOS mRNA formation in Kupffer cells by functionally inactivating NF-kappa B.

Attenuation of NF-kappa B activity by forskolin could occur by two alternative pathways: either forskolin could directly prevent the degradation of Ikappa Balpha proteins and thus the translocation of NF-kappa B into the nucleus, or forskolin could cause cytoplasmic retention of NF-kappa B by some other mechanism without preventing degradation of the Ikappa Balpha protein. LPS treatment of Kupffer cells decreased levels of cytoplasmic Ikappa Balpha , and this decrease coincided with the appearance of the p65 subunit of NF-kappa B in the nucleus. In cytoplasmic extracts of Kupffer cells that had been pretreated with forskolin there appeared to be no decrease in Ikappa Balpha upon stimulation with LPS (compare lanes 2 and 3, Fig. 7). These results indicate that forskolin protects Ikappa Balpha presumably by preventing its phosphorylation and degradation.

Nuclear NF-kappa B itself can induce the expression of the Ikappa Balpha gene, thus rapidly replenishing the depleted Ikappa Balpha protein pool in the cytoplasm of cells (31). The newly synthesized Ikappa Balpha proteins then complex with NF-kappa B in the cytoplasm, limiting its translocation into the nucleus. This Ikappa B homeostasis operates in LPS-stimulated Kupffer cells, where Ikappa Balpha mRNA levels remained elevated for longer than 6 h (Fig. 8, panel B); it provides the most likely explanation for why LPS-induced iNOS mRNA levels in Kupffer cells are not expressed indefinitely but were observed to decrease substantially by 24 h (Fig. 2, B and D). Forskolin alone induced transient Ikappa Balpha mRNA formation in Kupffer cells, with the mRNA levels peaking after 2 h of stimulation and decreasing to control values by 6 h (Fig. 8, panels A and D). Interestingly, pretreatment of Kupffer cells for 1 h with forskolin up-regulated LPS-induced Ikappa Balpha mRNA levels (Fig. 8, panels C and D). It is important to note that maximal up-regulation of Ikappa Balpha mRNA levels in Kupffer cells pretreated with forskolin for 1 h and then stimulated with LPS occurred after 1 h (Fig. 8, panel D), but in fact the Kupffer cells had been in contact with forskolin for 2 h, at which time forskolin induces maximum amounts of Ikappa Balpha mRNA in Kupffer cells (Fig. 8D). It may be of importance to consider that the time at which maximal inhibition of LPS-induced iNOS mRNA levels in Kupffer cells occurs is when the cells have been pretreated with forskolin for 1 h compared with the situation where forskolin was added after LPS (Fig. 3B), which coincides with maximal Ikappa Balpha mRNA levels in the cells. In contrast, when forskolin was added to Kupffer cells several hours after LPS, the transcriptional and translational pathways of iNOS formation are already well established, and thus there is no opportunity for forskolin to exert its inhibitory mode of action on iNOS synthesis.

Recent reports have shown that LPS stimulation of Kupffer cells activates NF-kappa B within 1 h, both in vitro (32, 33) and in the intact rat (34). Moreover, CD18/ICAM-1-dependent NF-kappa B activation leads to nitric oxide production in Kupffer cells (35). To our knowledge, the present report is the first detailed analysis of the NF-kappa B control system during iNOS response to endotoxin in the Kupffer cell. Also, this is the first report to characterize the effects of cAMP on this regulatory system; a previous study using Kupffer cells reported no effect of dibutyryl cAMP on LPS-stimulated iNOS induction (36).

The effects of cAMP on iNOS production are of increasing interest since the first report (37) that cAMP-elevating agents induced iNOS in cultured vascular smooth muscle cells and that this induction was synergistic with that elicited by inflammatory cytokines. cAMP elevation has been shown to have similar effects in renal mesangial cells (6) and in rat brown adipoctyes (38). Although cAMP alone does not induce iNOS in unstimulated cardiac myocytes, it augments iNOS induction in interleukin 1beta -stimulated cells (39). NF-kappa B/Rel is regulated positively by the cAMP cascade to help initiate iNOS gene expression in response to LPS stimulation of the macrophage line RAW 264.7, and inhibition of adenylate cyclase attenuates the activation of iNOS in these cells (40). In 3T3 fibroblasts different signaling pathways including elevation of cAMP lead to the induction of iNOS by NF-kappa B mediation (41). In contrast, elevation of cellular cAMP has been shown to down-regulate iNOS in endotoxin-activated cultures of rat microglia (quiescent brain macrophages (42)), rat primary astrocytes (43), and J774 cells (murine macrophage line (44)).

Our present findings suggest that pretreatment of Kupffer cells with forskolin both prevents the degradation of Ikappa Balpha and induces Ikappa Balpha mRNA formation, thereby increasing the pool of Ikappa Balpha proteins in the cytoplasm. When these same cells are stimulated with LPS, degradation of Ikappa Balpha proteins that are complexed with NF-kappa B subunits occurs, but the newly activated NF-kappa B proteins reassociate with the newly synthesized Ikappa Balpha proteins rapidly, thus limiting their translocation into the nucleus. A reduced translocation of NF-kappa B proteins into the nucleus results in decreased iNOS gene transcription and consequently low levels of iNOS formation.

The ability of cAMP to attenuate LPS-induced NO formation by Kupffer cells provides a model in which to characterize the intracellular signaling pathways that regulate iNOS gene expression in the liver under conditions of endotoxic shock. Oxidant stress up-regulates and antioxidants down-regulate NF-kappa B, and hypoxia alters cellular signal transduction in shock and sepsis (45). Treatment of Kupffer cells in vitro which models hypoxia/ischemia may permit systemic shock to be evaluated separately from simple endotoxin exposure; it will be of interest to determine whether these secondary shock-related effects on NF-kappa B are abrogated by cAMP elevation.

    ACKNOWLEDGEMENTS

We thank Lynnette Walters for isolating the Kupffer cells and for typing this manuscript. We are grateful to Dr. Katherine M. Howard for helpful comments and to Dr. Stephen A. K. Harvey for thoughtful criticism of the manuscript.

    FOOTNOTES

* This work was supported by National Institutes of Health Grant DK-33538 and by Robert A. Welch Foundation Grant AQ-0728.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Biochemistry, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78284.

1 The abbreviations used are: NO, nitric oxide; NOS, nitric-oxide synthase; eNOS, nNOS, and iNOS, endothelial, neuronal, and inducible NOS, respectively; LPS, lipopolysaccharide; PAGE, polyacrylamide gel electrophoresis; NF-kappa B, nuclear factor kappa B; TES, 2-{[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]]amino}ethanesulfonic acid; IBMX, 3- isobutyl-1-methylxanthine.

    REFERENCES
Top
Abstract
Introduction
Procedures
Results
Discussion
References

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