From the Department of Pharmacology, College of Medicine, State University of New York Health Science Center at Syracuse, Syracuse, New York 13210-2339
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ABSTRACT |
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The ability of cAMP-dependent protein
kinase (PKA) to phosphorylate type I, II, and III inositol
1,4,5-trisphosphate (InsP3) receptors was examined.
The receptors either were immunopurified from cell lines and then
phosphorylated with purified PKA or were phosphorylated in intact cells
after activating intracellular cAMP formation. The former studies
showed that the type I receptor was a good substrate for PKA (0.65 mol
Pi incorporated/mol receptor), whereas type II and III
receptors were phosphorylated relatively weakly. The latter studies
showed that despite these differences, each of the receptors was
phosphorylated in intact cells in response to forskolin or activation
of neurohormone receptors. Detailed examination of SH-SY5Y
neuroblastoma cells, which express 99% type I receptor, revealed
that minor increases in cAMP concentration were sufficient to cause
maximal phosphorylation. Thus, VIP and pituitary adenylyl cyclase
activating peptide (acting through Gs-coupled pituitary
adenylyl cyclase activating peptide-I receptors) were potent stimuli of
type I receptor phosphorylation, and remarkably, even slight increases
in cAMP concentration induced by carbachol (acting through
Gq-coupled muscarinic receptors) or other Ca2+
mobilizing agents were sufficient to cause phosphorylation. Finally, PKA enhanced InsP3-induced Ca2+ mobilization in
a range of permeabilized cell types, irrespective of whether the type
I, II, or III receptor was predominant. In summary, these data show
that all InsP3 receptors are phosphorylated by PKA, albeit
with marked differences in stoichiometry. The ability of both
Gs- and Gq-coupled cell surface receptors to
effect InsP3 receptor phosphorylation by PKA suggests that
this process is widespread in mammalian cells and provides multiple
routes by which the cAMP signaling pathway can influence
Ca2+ mobilization.
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INTRODUCTION |
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Inositol 1,4,5-trisphosphate (InsP3)1 receptors form tetrameric channels in endoplasmic reticulum membranes that conduct Ca2+ in an InsP3-sensitive manner (1-3). Thus, they link cell surface receptor-mediated increases in InsP3 formation to increases in cytoplasmic free Ca2+ concentration ([Ca2+]i). To date, the coding regions of three mammalian InsP3 receptor genes have been sequenced (4-11). Their products, termed type I, II, and III receptors, are ~2700 amino acids in length and are 60-70% identical at the amino acid level (2-11). Each receptor is thought to have the same overall structure, being divided into three domains: an N-terminal ligand binding domain, a C-terminal channel forming domain, and an intervening sequence, termed the coupling domain, that contains sites either known or hypothesized to be involved in receptor regulation (2-4, 6, 12). Type I, II, and III receptors are expressed in different amounts in different cell types, and some cells co-express all three receptors (13, 14). InsP3 receptors form heterotetramers in intact cells, and such associations persist after receptor solubilization (14-17).
Characterization of differences between type I, II, and III receptors is at a preliminary stage, and it is not yet clear what properties are conferred upon a cell by the selective expression of a particular receptor. Recent studies have indicated, however, that type I, II, and III receptors bind InsP3 with different affinities (8, 18, 19), raising the possibility that this could influence the potency of InsP3 as a Ca2+ mobilizing agent. Also, from sequence analysis it is considered likely that the receptors will differ in other ways; for example, in their ability to be phosphorylated by cAMP-dependent protein kinase (PKA) (2, 3, 8-11). Two serines within the PKA consensus sequence (R/K)(R/K)XS (20) are present in the rat type I receptor coupling domain (serines 1589 and 1755 in the sequences RRDS and RRES, respectively), and in vitro studies on purified rat type I receptor (21) have shown that both residues can be phosphorylated. These sites are also conserved in mouse and human type I receptors (4, 7), testifying to their importance. In contrast, neither consensus sequence is conserved in type II and III receptors (8-11), and although other serines in PKA consensus sequences are present elsewhere (8-11), it is not yet known whether type II and III receptors are substrates for PKA. In the present study we have examined the ability of PKA to phosphorylate type I, II, and III receptors by analyzing PKA-induced phosphorylation of purified receptors and the ability of agents that raise cAMP to cause receptor phosphorylation in intact cells.
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EXPERIMENTAL PROCEDURES |
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Cell Culture and Antisera-- SH-SY5Y human neuroblastoma cells, AR4-2J rat pancreatoma cells, and RINm5F rat insulinoma cells were obtained and cultured as monolayers in dishes (15 cm in diameter) as described (14). Rabbit polyclonal antisera termed CT1, CT2, and CT3 were raised against the C termini of rat type I, II, and III receptors, respectively, and were affinity purified and shown to be type-specific (14, 16, 22).
Phosphorylation of Purified InsP3
Receptors--
Receptors were immunoprecipitated from the three cell
lines (14, 16) and were phosphorylated in a manner similar to that described previously (23). After removal of culture medium, cells were
detached with 155 mM NaCl, 10 mM Hepes, 1 mM EDTA, pH 7.4 (HBSE), were centrifuged (500 × g for 2 min), and were disrupted with 12 ml of ice-cold
lysis buffer (50 mM Tris, 150 mM NaCl, 1%
Triton X-100, 1 mM EDTA, 0.2 mM
phenylmethylsulfonyl fluoride, 1 mM dithiothreitol, 10 µM leupeptin, 10 µM pepstatin, 0.2 µM soybean trypsin inhibitor, pH 8.0). After 30 min on
ice, cells were centrifuged (38,000 × g for 10 min at
4 °C). Supernatants were then incubated at 4 °C with either CT1,
CT2, or CT3 for 1 h and then for a further 1 h with protein
A-Sepharose CL-4B. Immune complexes were then isolated by
centrifugation (500 × g for 2 min), were washed twice
with ice-cold phosphorylation buffer (120 mM KCl, 50 mM Tris, 0.1% Triton X-100, 0.3 mM
MgCl2, pH 7.2), and were finally resuspended in
phosphorylation buffer. Aliquots of washed beads were then placed in
1.5-ml microfuge tubes together with [-32P]ATP (~5
µCi), 0-5 µM nonradioactive ATP, and 20 units of PKA catalytic subunit (final volume, 200 µl), were mixed gently, and were
then incubated at 30 °C. Reactions were stopped by adding 1.3 ml of
ice-cold phosphorylation buffer plus 1 mM ATP. Beads were
then centrifuged (16,000 × g for 10 s), were
washed twice with 1.5 ml of phosphorylation buffer plus ATP, and
finally were resuspended in 2× gel loading buffer (14).
Electrophoresis, Immunoblotting, and Autoradiography--
To
assess the concentration and phosphorylation of purified type I, II,
and III InsP3 receptors and the composition of
immunoprecipitates, samples of washed beads were electrophoresed in 4%
gels and were either silver-stained or immunoblotted as described (14,
16), the molecular mass and concentration of receptors being
established by comparison to standards of myosin (molecular mass, 205 kDa) and -galactosidase (molecular mass, 116 kDa). Radioactivity
associated with electrophoresed InsP3 receptors was
assessed initially by autoradiography of dried gels and then
quantitated by excision and scintillation counting of the
~240-280-kDa region.
Phosphorylation Stoichiometry--
Based on silver staining,
equal amounts of phosphorylated type I, II, and III receptor were
electrophoresed, and associated radioactivity was quantitated. The
number of moles of phosphate incorporated was then calculated from the
specific activity of the [-32P]ATP. The number of
moles of InsP3 receptor loaded onto the gel was determined
by measuring [3H]InsP3 binding to portions of
the receptor preparations destined for phosphorylation. Briefly, washed
beads in phosphorylation buffer were centrifuged (500 × g for 2 min) and were washed and resuspended in 20 mM Tris, 1 mM EDTA, pH 8.0, and were then
incubated with [3H]InsP3 as described (24).
The number of specific binding sites was then determined and was
assumed to equal the number of moles of InsP3 receptor.
These experiments also confirmed that the equal amounts of type I, II,
and III receptor defined by silver staining bound approximately equal
amounts of InsP3.
Phosphorylation of InsP3 Receptors in Intact
Cells--
This was assessed either directly after labeling cells with
32Pi or with a back-phosphorylation procedure
(23, 25), in which inhibition of PKA-catalyzed transfer of
32P from [-32P]ATP to purified receptors
reveals the degree to which PKA consensus sites are occupied by
nonradioactive phosphate in intact cells. In the former procedure,
cells were harvested in HBSE, were washed once with phosphate-free,
95% O2/5% CO2-gassed minimal essential medium, and were resuspended in the same medium, and 500-µl portions were incubated in microfuge tubes with ~125 µCi of
32Pi for 30-45 min at 37 °C. Cells were
then stimulated, were centrifuged (16,000 × g for
10 s), and were resuspended in 1 ml of ice-cold lysis buffer plus
1 mM Na3VO4, 100 mM
NaF, and 100 nM okadaic acid. InsP3 receptors
were then purified by immunoprecipitation with CT1, CT2, or CT3 and
protein A-Sepharose CL-4B and were electrophoresed. In the
back-phosphorylation procedure, cells were harvested in HBSE, were
washed once in gassed minimal essential medium, and were resuspended in
the same medium, and 500-µl portions were stimulated. Cells were then
centrifuged (16,000 × g for 10 s) and were
resuspended in 1 ml of ice-cold lysis buffer plus 1 mM Na3VO4, 100 mM NaF, and 100 nM okadaic acid. InsP3 receptors were then
purified by immunoprecipitation, were phosphorylated with [
-32P]ATP and PKA as described above, and were
electrophoresed.
cAMP Measurement-- Cells were harvested in HBSE, were washed and resuspended in gassed minimal essential medium (containing 0.25 mM 3-isobutyl-1-methylxanthine (IBMX) in some experiments), and were aliquotted into 100-µl portions. After 10 min at 37 °C, cells were stimulated for 2 min, and 100 µl of ice-cold 1 M trichloroacetic acid was added. After 15 min on ice, samples were centrifuged (16,000 × g for 3 min), and 160 µl of supernatant was removed and thoroughly mixed with 40 µl of 10 mM EDTA and 200 µl of freon/octylamine (1:1). After centrifugation (16,000 × g for 5 min), 100 µl of supernatant was neutralized with 50 µl of 25 mM NaHCO3, and cAMP content was measured by radioimmunoassay.
45Ca2+ Mobilization and Receptor Phosphorylation in Permeabilized Cells-- Cells (1-2 dishes) were harvested in 10 ml of HBSE, were centrifuged (500 × g for 2 min), were resuspended in 10 ml of ice-cold cytosol buffer (120 mM KCl, 2 mM KH2PO4, 2 mM MgCl2, 10 µM EGTA, 2 mM ATP, 20 mM Hepes, pH 7.0), were centrifuged again (500 × g for 2 min at 4 °C), and were resuspended in 0.8 ml of cytosol buffer. Cells were then permeabilized with nine discharges of a 3 microfarad capacitor (field strength, 3.75 kV/cm) as described (26), were diluted with cytosol buffer to 5 ml, were re-centrifuged, and were finally resuspended at 0.8-1 mg protein/ml in 1.3 ml of cytosol buffer. Suspensions were then incubated at 25 °C without or with 100 units/ml PKA for 10 min and then with ~0.3 µCi of 45Ca2+/ml for 10 min. Aliquots of cell suspension (90 µl) were then added to tubes containing 10 µl of either InsP3 or ionomycin and were incubated at 25 °C for 1 or 2 min, respectively. Incubations were terminated by addition of 4 ml of ice-cold cytosol buffer and immediate filtration through Whatman GF/B filters; radioactivity bound to filters was assessed after addition of 4 ml of Ecoscint H and overnight extraction. Radioactivity (the amount of 45Ca2+ remaining sequestered in the permeabilized cells or "45Ca2+ content") is expressed as a percentage of 45Ca2+ uptake (that remaining sequestered in the absence of stimulus). Importantly, uptake was unaffected by PKA; in the absence and the presence of PKA, respectively, 45Ca2+ uptake was 37 ± 5 and 38 ± 5 × 103 cpm for SH-SY5Y cells, 37 ± 1 and 35 ± 1 × 103 cpm for AR4-2J cells, and 34 ± 2 and 36 ± 2 × 103 cpm for RINm5F cells. In some experiments, control or PKA-treated permeabilized cells were centrifuged (16,000 × g for 10 s), and InsP3 receptors were immunoprecipitated and back-phosphorylated exactly as described for intact cells.
Materials--
Peroxidase-conjugated antibodies, molecular mass
markers, dithiothreitol, protease inhibitors, IBMX, ionomycin, phorbol
12-myristate 13-acetate, and receptor agonists/antagonists were
obtained from Sigma; forskolin was from Calbiochem; okadaic acid,
thapsigargin, and NaF were from Alexis Corp.;
[3H]InsP3 (21 Ci/mmol),
45CaCl2 (10 Ci/g), and
32Pi (H3PO4,
carrier-free) were from NEN Life Science Products; [-32P]ATP (~3000 Ci/mmol) was from Andotek; and PKA
catalytic subunit was from Promega.
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RESULTS |
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Phosphorylation of Immunoprecipitated Type I, II, and III
InsP3 Receptors--
Previous studies have shown that
enrichment of type I, II, and III InsP3 receptors in
SH-SY5Y, AR4-2J, and RINm5F cells, respectively, makes these cells
convenient starting points for InsP3 receptor purification
(14, 16). Because SH-SY5Y cells contain 99% type I receptor, a
preparation composed solely of type I receptor can be
immunoprecipitated from these cells with antiserum CT1 (14, 16). Type
II and III receptor preparations immunoprecipitated from AR4-2J and
RINm5F cells with antisera CT2 and CT3 are, in contrast, not
homogeneous because they are "contaminated" with traces of
co-immunoprecipitating type I receptor, which represents 12 and 4% of
total receptor in these cell lines (14, 16).
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InsP3 Receptor Phosphorylation in Intact SH-SY5Y Cells-- Because the type I receptor is a good substrate for PKA in vitro (Figs. 1 and 2), we examined whether it is phosphorylated in intact SH-SY5Y cells in response to cAMP elevation using either a back-phosphorylation procedure (23, 25) or 32Pi labeling of intact cells. Fig. 3A shows that vasoactive intestinal peptide (VIP), which stimulates cAMP levels in SH-SY5Y cells (27), causes type I receptor phosphorylation in intact cells because back-phosphorylation was inhibited (lanes 1-5). Pituitary adenylyl cyclase activating peptide (PACAP), which belongs to the same neurohormone family as VIP (28), had a similar effect (lanes 6-8). Because IC50 values for VIP and PACAP were 7 and 0.15 nM, respectively (Fig. 3B), and PACAP-I receptors bind PACAP with ~100-fold higher affinity than VIP (28, 29) and are present in SH-SY5Y cells (29), it is likely that the effects of both PACAP and VIP are mediated by these receptors. Fig. 3C shows that forskolin, which also elevates cAMP levels in SH-SY5Y cells (30, 31), similarly inhibited back-phosphorylation (maximally by ~60%). This indicates that the effects of VIP and PACAP are meditated by increases in cAMP alone, a view supported by findings that 1 mM dibutyryl cAMP inhibited back-phosphorylation by 65 ± 4%, whereas 1 mM dibutyryl cGMP inhibited by only 20 ± 4% (mean ± S.E., n = 4).2
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InsP3 Receptor Phosphorylation in Intact AR4-2J and RINm5F Cells-- Given the limited extent to which type II and III receptors are phosphorylated in vitro (Fig. 1), we examined whether they are phosphorylated in intact cells. Fig. 4A (lanes 1-4) shows that agents likely to raise cAMP levels in AR4-2J cells (34) inhibit the back-phosphorylation of both type I and II receptors to a similar extent, maximally by ~60% (Fig. 4B). Similarly, agents likely to elevate cAMP levels in RINm5F cells (35) inhibit the back-phosphorylation of both type I and III receptors, by far the strongest effect (~75% inhibition) being seen with forskolin (Fig. 4B). Thus, type II and III receptors are phosphorylated in intact cells, a finding confirmed by the fact that the 32P-content of type II receptors is increased by VIP, forskolin and PACAP in 32Pi-labeled AR4-2J cells (Fig. 4C, lanes 1-4) and that forskolin clearly increases the 32P content of type III receptors in 32Pi-labeled RINm5F cells (lanes 5-8).
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Agonist-induced Changes in cAMP Concentration-- We next examined cAMP levels in the three cell types to establish the extent to which VIP, PACAP, and forskolin were stimulatory and whether [Ca2+]i-elevating agents might also raise cAMP levels, because this could account for the effects of carbachol, ionomycin and thapsigargin on phosphorylation (Fig. 3D).
In SH-SY5Y cells in the absence of IBMX (Fig. 5A, lower panel), VIP and PACAP increased cAMP levels only modestly (by ~100%). Thus, IBMX was included to amplify agonist effects and facilitate accurate measurement of potency. In the presence of IBMX (Fig. 5A, upper panel), VIP and PACAP produced ~1,000 and 1,200% increases with EC50 values of 580 and 17 nM, respectively. Interestingly, VIP and PACAP exhibited the same ~50-fold potency difference as that seen for phosphorylation (Figs. 3B), but the absolute IC50 values for inhibition of back-phosphorylation were ~100-fold lower than the absolute EC50 values for stimulation of cAMP formation. Thus, a receptor reserve exists for phosphorylation and submaximal increases in cAMP concentration are sufficient to cause maximal phosphorylation. Carbachol also elevated cAMP levels (Fig. 5A), by ~35% in the absence of IBMX and by ~130% in the presence of IBMX (EC50 = 0.27 µM). This modest effect was blocked by atropine (Fig. 5A) and was mimicked by ionomycin (0.2 µM) and thapsigargin (1 µM), which produced ~170 and ~180% increases, respectively.2 Thus, the effect of carbachol on cAMP levels appears to result from elevation of [Ca2+]i, and InsP3 receptor phosphorylation correlates with increases in cAMP concentration.
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Consequences of Receptor Phosphorylation-- To establish whether InsP3 receptor function was modulated by PKA, we examined whether PKA altered InsP3-induced Ca2+ mobilization in permeabilized cells. Firstly, however, we sought to establish that PKA could enter and phosphorylate InsP3 receptors in permeabilized cells. This was found to be the case (Fig. 6A, inset), because back-phosphorylation of type I receptor was much greater in control permeabilized SH-SY5Y cells (lane 1) than in permeabilized cells exposed to PKA (lane 2), and analogous results were obtained for permeabilized AR4-2J and RINm5F cells.2 In each cell type, PKA significantly enhanced the potency of InsP3 by ~20% (Fig. 6, A-C) and in SH-SY5Y cells also caused an ~10% increase in maximal response (Fig. 6A). These effects on InsP3 action were truly PKA-dependent, because they were blocked if PKA was denatured by heating (see Fig. 6A legend), a manipulation that also blocked the kinase activity of PKA (Fig. 6A, inset, lane 3). Further, PKA did not enhance ionomycin-induced 45Ca2+ release (Fig. 6, A-C) or alter Ca2+ uptake (see Experimental Procedures), showing that the PKA-mediated modification of InsP3 action was not due to a nonspecific change in Ca2+ store characteristics. Finally, combination of the data in Fig. 6 (n = 4) with other independent determinations of InsP3 potency yielded the following EC50 values for InsP3 in the absence or the presence of PKA; for SH-SY5Y cells, 82 ± 4 and 68 ± 3 nM (n = 12), for AR4-2J cells, 72 ± 3 and 56 ± 2 nM (n = 11), and for RINm5F cells, 400 ± 35 and 301 ± 18 nM (n = 10), respectively, the effect of PKA being significant in all cell types (p < 0.05, by unpaired t test). That InsP3 exhibits relatively low potency in RINm5F cells (Fig. 6C) appears to reflect the predominance in this cell type of type III receptor, which has a lower affinity for InsP3 than type I or II receptors (8, 18, 19, 45).
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DISCUSSION |
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The major findings presented herein are (i) that all of the known InsP3 receptor types are phosphorylated by PKA in vitro, albeit inefficiently in the case of type III and particularly type II receptors, (ii) that each of the receptors is phosphorylated in intact cells in response to neurohormone receptor activation, (iii) that slight changes in intracellular cAMP concentration cause maximal phosphorylation, and (iv) that PKA-dependent phosphorylation enhances Ca2+ mobilization irrespective of which InsP3 receptor type is predominant.
It is clear from these and previous findings (2, 3), that the type I receptor is an excellent substrate for PKA. Previous measurements of phosphorylation stoichiometry in vitro using column chromatography-purified cerebellar type I receptors showed that although relatively high PKA concentrations incorporated 2 mol Pi/mol receptor, consistent with the presence of two serines within PKA consensus sequences (2, 3), lower PKA concentrations phosphorylated only serine 1755 and thus incorporated only 1 mol Pi/mol receptor (21, 36). Recent studies have also indicated that serine 1755 is phosphorylated preferentially in intact cells (37). Our value of 0.65 mol Pi/mol SH-SY5Y cell type I receptor is consistent with the view that only one serine, presumably serine 1755, is phosphorylated. It is possible that the incorporation is < 1 mol/mol in our studies because some of the PKA sites are already occupied by nonradioactive phosphate groups, an argument supported by the fact that the type I receptor is clearly a phosphoprotein in unstimulated cells (Fig. 3E, lane 1). These phosphates remain receptor-associated during immunoprecipitation (Figs. 1-4) but may not survive purification by column chromatography (21, 36).
Our work also shows, for the first time, that PKA phosphorylates type II and III receptors, albeit with low efficiency (0.04 and 0.14 mol Pi/mol receptor, respectively). This was not an artifact resulting from the incubation of purified receptors and kinases, because both receptors were phosphorylated in intact cells, most notably in response to forskolin. The identity of the sites phosphorylated and the reason why the phosphorylation stoichiometry is so low were not examined in the present study. Regarding the first point, however, although the sites phosphorylated in the type I receptor are not conserved in type II or III receptors, other serines within the PKA consensus sequence (R/K)(R/K)XS (20) are present in the coupling domains of type II and III receptors: in rat and human type II receptors at serine 1687 (8, 10), in rat and human type III receptors at serines 934 and 1133, and in rat type III receptor at serine 1460 (9-11). Thus, it is quite plausible that type II and III receptors are PKA substrates. Regarding the stoichiometry, the low values for type II and III receptors cannot be explained by occupation of phosphorylation sites by nonradioactive phosphate, because the receptors were not heavily phosphorylated in 32Pi-labeled resting cells. Rather, either the conformation of the type II and III receptors or their orientation when tetramerized may make phosphorylation of the consensus sequences unfavorable.
Overall, our studies in intact cells show that modest increases in cAMP concentration result in type I, II, and III InsP3 receptor phosphorylation. That the type I receptor is phosphorylated in SH-SY5Y cells is, to our knowledge, the first demonstration of this modification in intact neuronal cells and a number of aspects of this work are worthy of comment. Firstly, very modest increases in cAMP concentration are sufficient to maximally stimulate InsP3 receptor phosphorylation. Thus, a receptor reserve is apparent for VIP and PACAP, which via Gs-coupled PACAP-I receptors activate adenylyl cyclase (28, 29) to produce >10-fold increases in cAMP concentration. In contrast, activation of m3 receptors produced much smaller increases in cAMP concentration, and thus, half-maximal values for carbachol-induced phosphorylation and cAMP formation were similar. The mechanism by which carbachol increases cAMP levels is intriguing because m3 receptors are coupled via Gq to phosphoinositidase C (33). Previous studies in SH-SY5Y cells (30, 31), however, indicate that cAMP elevation can result from activation of Ca2+-dependent adenylyl cyclases, which are abundant in neuronal cells (38, 39). Our observation that ionomycin and thapsigargin elevate cAMP levels supports this view and suggests that carbachol, by elevating [Ca2+]i (33), stimulates Ca2+-activated adenylyl cyclases, which are likely to be present in SH-SY5Y cells (39). Thus, in SH-SY5Y cells and possibly other neuronal cells, cell surface receptors coupled to either Gs or Gq can increase cAMP levels and thus PKA-dependent type I InsP3 receptor phosphorylation. Although the effects of this phosphorylation are not currently clear (2, 3), these data make it apparent that analyses of Ca2+ mobilization in neuronal cells in response to agonists that act via Gq-coupled receptors (e.g. carbachol) should take into account the possibility that type I receptors become phosphorylated at the same time that InsP3 is activating InsP3 receptors.
At present, there is little consensus regarding the effects of PKA on InsP3 receptor function (3). On one hand, PKA enhances the effects of InsP3 on purified reconstituted cerebellar type I receptors (40) and appears to enhance InsP3 action in intact hepatocytes (41). On the other hand, PKA inhibits the effects of InsP3 on cerebellar microsomes (42) and appears to decrease sensitivity to InsP3 in platelets (43) and trachea (44). Our studies indicate that PKA causes a modest (~20%) enhancement of InsP3 potency in SH-SY5Y, AR4-2J, and RINm5F cells and also enhances by ~10% the maximal effect of InsP3 in SH-SY5Y cells. It is noteworthy that the dual effect of PKA on SH-SY5Y cells parallels the modest effects of PKA on purified cerebellar type I InsP3 receptor (40).
Thus, PKA enhances InsP3 action irrespective of the fact that SH-SY5Y, AR4-2J, and RINm5F cells express different receptor complements. Although it might be expected that InsP3 potency would be affected in SH-SY5Y and RINm5F cells in which a relatively large proportion of the receptors are phosphorylated, it is somewhat surprising that a potency shift was seen in AR4-2J cells in which the predominant InsP3 receptor type is very weakly phosphorylated. It may be significant though that this cell type contains 12% type I receptor (14), which is a excellent substrate for PKA and which forms heterotetramers with type II receptors (14, 16, 17). Perhaps such heterotetramers are affected by PKA to a similar extent as, or even more than, homotetramers of type I receptor. This is an intriguing possibility, particularly because hepatocytes express type I and II receptors in similar proportion to AR4-2J cells (13, 14, 16), and Ca2+ signaling in hepatocytes appears particularly sensitive to PKA (2, 41).
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ACKNOWLEDGEMENTS |
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We thank Carol Jones for performing cAMP assays and Grant Kelley, Jon Oberdorf, and Chang-Cheng Zhu for many helpful discussions.
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FOOTNOTES |
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* This work was supported by Grant DK49194 from the National Institutes of Health, the Sinsheimer Fund, and a grant-in-aid from the American Heart Association (New York State Affiliate, Inc.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Pharmacology,
College of Medicine, SUNY Health Science Center at Syracuse, 750 East
Adams St., Syracuse, NY 13210-2339. Tel.: 315-464-7956; Fax:
315-464-8014; E-mail: WOJCIKIR{at}VAX.CS.HSCSYR.EDU.
1 The abbreviations used are: InsP3, inositol 1,4,5-trisphosphate; PKA, cAMP-dependent protein kinase; IBMX, 3-isobutyl-1-methylxanthine; VIP, vasoactive intestinal peptide; PACAP, pituitary adenylyl cyclase activating peptide.
2 R. J. H. Wojcikiewicz and S. G. Luo, unpublished data.
3 That the radioactivity seen in Fig. 3E (lane 1) reflects metabolic incorporation of 32P was demonstrated by the fact that addition of 32Pi to cells immediately prior to cell lysis did not lead to the recovery of radioactive receptors.
4 R. J. H. Wojcikiewicz and S. G. Luo, submitted for publication.
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REFERENCES |
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