From the Institut Cochin de Génétique
Moléculaire, U363 INSERM, Hôpital Cochin, 27 rue du
Faubourg Saint Jacques, 75014 Paris, ¶ Laboratoire de Biologie et
Ultrastructure du Noyau, UPR9044 CNRS, BP 8, 94801 Villejuif Cedex, and
Institut Curie, U248 INSERM, 26 rue d'Ulm,
75005 Paris, France
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ABSTRACT |
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The vav proto-oncogene is exclusively expressed in hematopoietic cells and encodes a 95-kDa protein that contains multiple structural domains. Vav is involved in the expansion of T and B cells, in antigen-mediated proliferative responses, and in the induction of intrathymic T cell maturation. It becomes rapidly and transiently tyrosine-phosphorylated upon triggering of a large number of surface receptors and catalyzes GDP/GTP exchange on Rac-1. We now provide evidence for the specific interaction of Vav with heterogeneous nuclear ribonucleoprotein (hnRNP) C. Vav and hnRNP C interact both in vivo and in vitro mediated through the carboxyl Src homology 3 domain of Vav and the proline-rich motif located in the nuclear retention sequence of hnRNP C. More importantly, Vav-hnRNP C complexes are present in living hematopoietic cells and both proteins localize in the nuclei, mainly on perichromatic fibrils but also on clusters of interchromatin granules. The Vav-hnRNP C interaction is regulated by poly(U) RNA, although a basal association is still detected in the absence of RNA. Furthermore, RNA homopolymers differentially alter the binding affinity of Vav to hnRNP C and hnRNP K. We propose that Vav-hnRNP interactions may be established in an RNA-dependent manner.
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INTRODUCTION |
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Oncogenic vav was originally isolated on the basis of its transforming properties in NIH3T3 fibroblasts (1). The corresponding cellular gene, the proto-oncogene vav, is only expressed in cells of hematopoietic lineage (2). However, recently, a homologue of vav, vav2, has been identified and is more widely expressed (3, 4). Vav and Vav2 have over 50% identical residues, and both harbor a number of conserved domains found in molecules involved in protein-protein interactions and signaling events (Fig. 1A) (see Ref. 5 for review).
Vav becomes rapidly and transiently tyrosine-phosphorylated upon
triggering of a variety of surface receptors in almost every hematopoietic cell. These include T cell receptor/CD3, CD2, and CD28 on
T cells, IgM receptor and CD19 on B cells, the IgE high affinity
FcRI receptor on mast cells, the lipopolysaccharide receptor on
monocytes/macrophages, and several cytokine and tyrosine kinase
receptors (5). The rapid tyrosine phosphorylation of Vav in all these
cell types suggests an important role for this protein in signal
transduction of hematopoietic cells. The Syk-Zap protein-tyrosine
kinases have recently been shown to participate in tyrosine
phosphorylation of Vav (6). Vav also binds to a hematopoietic specific
protein, SLP-76, and after antigen receptor activation, the two
proteins synergistically activate NF-AT and IL-2
gene expression (7). Furthermore, chimeric
vav
/
rag-2
/
mice
have shown that Vav participates in the expansion of both T and B cells
and is essential for antigen-mediated proliferative responses and for
induction of intrathymic T cell maturation (8-10).
Despite the importance of Vav, little is known about its function. Recently, it has been shown that tyrosine-phosphorylated Vav catalyzes GDP/GTP exchange on Rac-1. Rac-1 is implicated in cell proliferation and cytoskeletal organization, and stimulates c-Jun kinase, a downstream element in the signaling pathway of this GTPase (11, 12). As Vav exhibits a striking combination of structural motifs and is localized both in the cytoplasm and in the nucleus (13, 14), it is likely that Vav is involved in several distinct cellular functions. It has been reported that the carboxyl SH31 (C-SH3) of Vav interacts with the proline-rich sequences of hnRNP K, which has a role in the regulation of the late steps of RNA biogenesis and transport of mRNA (15, 16). This interaction has been detected mainly in the cytoplasmic fraction (16). On the other hand, we have shown that the same C-SH3 domain of Vav also interacts with Ku-70 independently of the presence of a proline motif (14). Ku-70 is the DNA-binding element of the DNA-dependent protein kinase, which has been implicated in DNA repair, replication, recombination, and transcription. In addition, it has recently been shown that the leucine zipper motif of Vav associates with ENX-1 (17). enx-1 is a novel human gene homolog of the Drosophila Enhancer of Zeste gene, a member of the Polycomb group of genes, which code for transcriptional regulators of homeobox gene expression.
To further identify proteins capable of physically associating with Vav, we studied a positive clone isolated from a Jurkat cDNA library by the yeast two-hybrid system using as bait the SH2 and SH3 domains of Vav. In this paper, we provide evidence for the interaction of Vav with the heterogeneous nuclear ribonucleoprotein (hnRNP) C. hnRNP C proteins (C1 of 41,000 and C2 of 43,000 Mr proteins) are among the most abundant hnRNPs which bind to nascent pre-messenger RNA at an early posttranscriptional stage and are exclusively localized in the nuclei (see Ref. 18 for review). In particular, they bind strongly to the intron polypyrimidine tract of pre-mRNA (19), and this binding is negatively regulated by serine/threonine hyperphosphorylation (20). In vitro, hnRNP C proteins have been involved in pre-mRNA splicing (21). Using a variety of approaches, we characterized the Vav-hnRNP C association and analyzed the influence of RNA homopolymers on this interaction as well as the potential role of Vav as a hnRNP-interacting molecule.
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EXPERIMENTAL PROCEDURES |
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Plasmids, Cloning, and Mutagenesis-- Yeast pVJL10 derivatives included those containing SHVAV (residues 623-837 of human Vav), point mutant SHVAV P833L, SH3-C Vav (residues 787-837), SH3-C' Vav (residues 813-837), and SH3-N'/SH2 Vav (residues 623-812) (14). Plasmids encoding the RasV12 and Raf proteins were used as controls (14). A bacterial pGEX2T derivative containing Vav SH2/SH3-C (residues 666-840) (15) was also used. v90 from pGAD-v90 (residues 120-290 of hnRNP C1) was cloned in pGEX-4T-2 (Pharmacia Biotech Inc.) digested with EcoRI and NotI endonucleases. The mutants SHVAV Y836F and v90PA were constructed in the yeast two-hybrid vectors pVJL10-SHVAV and pGAD-v90, respectively, using the Transformer site-directed mutagenesis kit from CLONTECH. The mutagenic primers were 5'-C TGG TTC CCT GCC AAC TTC GTG TGA GTC GAC CTG CAG CC-3', for SHVAV Y836F, and 5'-CCA GCA CGT GTA GCT GCT GCA GCT GCT ATT GCT CGG GC-3', for v90PA. The mutated bases are underlined.
Library Screening--
Saccharomyces cerevisiae
strain L40 (MATa trp1 leu2 his3
LYS2::lexA-HIS3 URA3::lexA-lacZ) was grown at
30 °C in YPD medium containing 1% yeast extract, 2% polypeptone,
and 2% glucose, and sequentially transformed with pVJL10-SHVAV and a
Jurkat cell cDNA library (14). Double transformants were
plated on yeast drop-out medium lacking Trp, Leu, His, Lys, and uracil
(22). They were grown for 5 days at 30 °C, after which colonies were patched on the same medium and replica-plated on Whatman 40 filters to
test for -galactosidase activity (23). Positive clones were rescued
and tested for specificity by retransformation into L40 either with
pVJL10-SHVAV or with extraneous targets (pLexA-RasV12,
pLexA-Lamin, or pVJL10) (14).
Sequence Analysis-- Sequences of point mutations and cDNA inserts from positive clones of the two-hybrid screening were performed on both strands with an automatic sequencer (Applied Biosystems, model 373A) using the Sanger dideoxy-termination method (24). Sequence comparisons were done with the FASTA program.
Cell Culture and Lysis--
Jurkat cells (clone J77.6.8) were
grown in RPMI 1640 medium (Life Technologies, Inc.) supplemented with
10% heat-inactivated fetal calf serum (Boehringer Mannheim), 2 mM L-Glu, penicillin, and streptomycin, in a
5% CO2 humidified atmosphere at 37 °C. UT7-S Epo cells
(25) were maintained in -medium (Life Technologies, Inc.) with 10%
fetal calf serum and erythropoietin at 1-2 units/ml. Cell lysis was
performed with 108 cells/ml at 4 °C in 150 mM NaCl, 50 mM HEPES (pH 7.4), 1 mM
sodium vanadate, 10% glycerol, 0.875% Brij 96 (polyoxylethylene-10-oleylether), 0.125% Nonidet P-40, 1% aprotinin,
1 mM phenylmethylsulfonyl fluoride, 1 mg/ml pepstatin, and
1 mg/ml leupeptin for 20 min. The extract was centrifuged at
20,000 × g for 20 min, and the supernatant was placed
in N2 and stored at
80°C.
Subcellular Fractionation--
Jurkat and UT7-S Epo cells were
prepared according to Ref. 26, with minor modifications. Briefly, cells
grown to mid-log phase were harvested by centrifugation and washed with
cold phosphate-buffered saline (PBS). All subsequent steps were
performed on ice. The pellet was resuspended in ice-cold buffer A (10 mM HEPES (pH 7.9), 1.5 mM MgCl2, 10 mM KCl, and 0.5 mM dithiothreitol). Cells were allowed to stand for 10 min (or until no intact cells were observed under the microscope). Nuclei were collected by centrifugation at 2000 rpm for 10 min at 4 °C. The supernatant (cytoplasmic extract) was
mixed with buffer B (0.3 M HEPES (pH 7.9), 1.4 M KCl, and 0.03 M MgCl2) and
centrifuged at 100,000 × g for 60 min at 4 °C. The
high speed supernatant was designated as the S100 fraction. Pelleted
nuclei were recentrifugated at 25,000 × g for 20 min at 4 °C to remove residual cytoplasmic material. These crude nuclei were resuspended in buffer C (20 mM HEPES (pH 7.9), 25%
glycerol, 0.42 M NaCl, 1.5 mM
MgCl2, 0.2 mM EDTA, 0.5 mM
phenylmethylsulfonyl fluoride, and 0.5 mM dithiothreitol)
and nuclear protein extracted on ice for 30 min. Nuclear extract was
obtained by centrifugation at 25,000 × g for 20 min at
4 °C. S100 and nuclear fractions were dropped in liquid
N2 and stored at 80 °C.
Affinity Chromatography--
Expression of the
glutathione-S-transferase (GST) fusion proteins was induced
4 h at 30 °C by the addition of 1 mM
isopropyl-1-thio--D-galactopyranoside for GST-v90 and
125 nM for GST-Vav SH2/SH3-C. The fusion proteins were
isolated from bacterial lysates by affinity chromatography with
glutathione-Sepharose beads (Pharmacia). Lysates from Jurkat and UT7-S
Epo cells (107 or 2 × 107) were incubated
for 2 h with fusion protein (0.5-1 µg) bound to
glutathione-coupled Sepharose beads. The beads were washed six times in
lysis buffer and dissolved into sodium dodecyl sulfate (SDS)-sample
buffer, incubated at 95 °C for 10 min, and subjected to
SDS-polyacrylamide gel electrophoresis (PAGE).
Electrophoresis and Western Blot Analysis-- The gels were electroblotted onto nitrocellulose membranes and probed with the different antibodies. Peroxidase-coupled anti-rabbit IgG from donkey, anti-mouse IgG from sheep, and anti-rat IgG from sheep were from Amersham (Les Ulis, France). Immunoreactive bands were visualized using an enhanced chemiluminescence Western blotting system (ECL, Amersham) according to the manufacturer's protocol.
Antibodies-- Anti-Vav polyclonal antibodies were from Dr. A. Altman2 and Drs. M. Barbacid and X. R. Bustelo (Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton) (27). Anti-Vav polyclonal antibody for electron microscopy was from Transduction Laboratories (Lexington, KY). Anti-Vav monoclonal antibody was from UBI (Lake Placid, NY). Anti-Raf1 polyclonal antibody was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Monoclonal anti-hnRNP C (4F4) antibody was provided by Dr. G. Dreyfuss (University of Pennsylvania) (28), N3H10 (anti-Ku-70) monoclonal antibody by Dr. W. H. Reeves (University of North Carolina) (29), and anti-hnRNP K (B4B6) monoclonal antibody by Drs. J. Celis and K. Dejgaard (30).
Electron Microscopy--
After centrifugation of UT7-S Epo
cells, the cell pellets were fixed for 1 h at 4 °C in 4%
paraformaldehyde in 0.1 M Sörensen phosphate buffer,
pH 7.3. After another centrifugation at the end of fixation, the
resulting pellets were washed with phosphate buffer for 1 h at
4 °C, dehydrated in increasing concentrations of methanol (30, 50, 70, and 90%), and embedded in Lowicryl K4M (31). Polymerization was
carried out under long wavelength UV light (Philips fluorescence tubes
T6, 6 watts) for 5 days at 20 °C and for 1 day at room
temperature. Ultrathin sections were mounted on carbon-Formvar-coated
gold grids (200-mesh). For immunolabeling, thin sections were incubated
for 30 min at room temperature either on a drop of anti-Vav polyclonal
antibody (diluted 1/50 in PBS) or anti-hnRNP C monoclonal antibody
(diluted 1/5000). After 15 min in PBS, the grids were floated for 10 min on a drop of either 1/50 dilution of anti-rabbit IgG or 1/50 of
anti-mouse IgG. Both second antibodies being conjugated to gold
particles 10 nm in diameter (Biocell Research Laboratories, Cardiff,
United Kingdom). After a final PBS wash, the grids were rapidly rinsed
in distilled water and air-dried. Before observation, grids were
processed according to the RNP preferential staining method of Bernhard (32). For controls, a 1/10 dilution of normal rabbit serum or 1/10
dilution of normal mouse ascites fluid were used instead of anti-Vav
and anti-hnRNP C, respectively.
Competition Experiments-- The peptide blocking experiments were done with GST fusion proteins preincubated for 30 min at 4 °C with 50-100 µM amounts of the polyproline peptide YPARVPPPPPIARAV derived from hnRNP C proteins (residues 126/139-140/153 of hnRNP C1/C2, respectively). For RNA experiments, nuclear extracts were preincubated for 30 min at 4 °C with poly(U), poly(rA), poly(rG), or poly(rC) RNA (Pharmacia) or nuclear RNA (preheated 10 min at 65 °C) at several concentrations before adding antibodies for coimmunoprecipitation experiments.
Coimmunoprecipitation Experiments-- Nuclear extracts from Jurkat and UT7-S Epo cells (1.5-3 × 107) were diluted 2.5 times with buffer C (see "Subcellular Fractionation") lacking NaCl and supplemented with 0.05% Brij 96. After centrifugation, supernatants were incubated with preimmune serum for 30 min and protein A-Sepharose beads (Pharmacia) for 1 h at 4 °C. Supernatants were incubated overnight with polyclonal anti-Vav or preimmune serum, followed by protein A-Sepharose beads for 1 h. After centrifugation, the beads were washed six times in PBS, 10% glycerol, 1% aprotinin, and 1 mM phenylmethylsulfonyl fluoride. Eluted proteins were dissolved into SDS-sample buffer, incubated at 95 °C for 10 min, and subjected to SDS-PAGE. Equivalent amounts of S100 fractions were directly used for coimmunoprecipitation experiments.
Isolation of Nuclear RNA and RNase Digestion-- Nuclear RNA was prepared by the acid/guanidinium isothiocyanate/phenol/chloroform extraction method (33). Digestions with RNase (400 µg/ml) were carried out with preboiled bovine RNase I A (Pharmacia) for 45 min at 4 °C. After centrifugation, the supernatants were used for coimmunoprecipitation experiments.
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RESULTS |
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Interaction of Vav and hnRNP C-- To study the signaling mechanism involved in hematopoietic cell development, we searched for proteins bound to Vav using the yeast two-hybrid approach. A Jurkat T cell cDNA library was screened using as bait SHVAV (residues 623-837 of human Vav) fused to the carboxyl terminus of the LexA DNA-binding domain. This portion of Vav includes the SH2 domain flanked by two SH3 domains (Fig. 1A), structures commonly involved in protein-protein interactions of signaling molecules. The v90 polypeptide was identified as interacting specifically with SHVAV both by the induction of lacZ expression and by the growth of yeast in absence of histidine, the two reporter genes used in the two-hybrid system. The specificity of this interaction was confirmed because neither the RasV12/v90 polypeptide nor the LexA DNA-binding domain/v90 polypeptide transactivated the reporter genes (Fig. 1B). Furthermore, full-length Csk, another protein with SH2 and SH3 domains, did not interact with the v90 polypeptide, whereas the full-length Vav was clearly positive (data not shown).
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The C-SH3 Domain of Vav Is Involved in the Interaction with hnRNP C-- To further delineate the Vav interacting domain, three fragments of SHVAV cDNA subcloned in frame with the LexA DNA-binding domain were used (14): the intact C-SH3 of Vav (SH3-C Vav), the COOH-terminal half of Vav C-SH3 (SH3-C' Vav), and the SHVAV lacking the COOH-terminal half of C-SH3 (SH3-N'/SH2). The study of these subclones by the two-hybrid system showed that the C-SH3 of Vav is responsible for the interaction with v90 polypeptide (Fig. 2A). The SH3-C' Vav was still able to bind the COOH-terminal region of hnRNP C, but SH3-N'/SH2 Vav could not interact with the v90 polypeptide.
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The Proline-rich Motif of hnRNP C Is Implicated in the Binding to Vav-- We next sought to identify the hnRNP C sequence involved in the interaction with Vav. hnRNP C proteins have a proline-rich sequence stretch, YPARVPPPPPIARAV (35) (see Fig. 1C), which resembles previously identified SH3 binding motifs (39) and is contained in the v90 subclone. We examined whether this proline-rich sequence was responsible for the Vav-hnRNP C interaction.
First, the ability of GST-Vav SH2/SH3-C (15) to bind hnRNP C was tested. As hnRNP C proteins are completely restricted to the nucleus (36), subcellular fractionations were performed (see "Experimental Procedures"). The purity of the fractions were assessed on Jurkat and UT7-S Epo cells by the absence of Raf proteins in the nuclei fraction and the absence in the cytoplasmic fraction of hnRNP C (Fig. 3, A-C). Nuclear extracts from Jurkat cells were incubated with Sepharose beads carrying GST-Vav and the filters blotted with anti-hnRNP C monoclonal antibody. In these conditions hnRNP C interacted with GST-Vav (Fig. 3D, third lane). On lighter exposures, the closely migrating hnRNP C1 and C2 proteins were both detected. No interaction was detected with GST alone. Similar results were obtained with extracts from UT7-S Epo cells (data not shown). Therefore, the chimeric GST-Vav SH2/SH3-C recognized full-length human hnRNP C proteins in vitro.
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In Vivo Binding of Vav and hnRNP C-- To examine whether Vav and hnRNP C formed complexes in intact hematopoietic cells, we performed a series of coimmunoprecipitation experiments. For this purpose, nuclear extracts from UT7-S Epo cells were incubated with an anti-Vav polyclonal antibody (raised against a specific peptide of Vav corresponding to residues 456-469) or with the preimmune serum. The resulting immunocomplexes were blotted with anti-hnRNP C and, as illustrated in Fig. 4 (right panel), Vav coimmunoprecipitated with the endogenous hnRNP C proteins. Coimmunoprecipitations using the S100 fractions were performed as controls and no hnRNP C was detected. In fact, hnRNP C proteins were not present in lysates from S100 fractions of UT7-S Epo cells (Fig. 4, left panel), whereas Vav was detected in both fractions (data not shown). Similar experiments were carried out with a second anti-Vav polyclonal antibody, which recognizes a different epitope (27), and with extracts from Jurkat cells, and the results were equivalent to those described above (data not shown). In similar experiments, we also detected Vav proteins in the anti-hnRNP C immunoprecipitates. Thus, our findings show that full-length Vav and hnRNP C can form a complex in the nucleus of intact hematopoietic cells.
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Nuclear Localization of Vav and hnRNP C-- By confocal microscopy, we have shown previously that Vav is partially localized in the nucleus of UT7-S Epo cells (14). To evaluate the in vivo association of Vav and hnRNP C, the localization of both proteins in the nucleus of UT7-S Epo cells was observed by electron microscopy. After EDTA staining, chromatin was bleached while nuclear RNP components, including nucleolar and extranucleolar RNP components, remained contrasted (see "Experimental Procedures"). The localization of proteins recognized by anti-Vav and anti-hnRNP C antibodies were similar except that the former was always less intense (Fig. 5, A and B). In both cases, gold particles were clearly localized on perichromatin fibrils extending in the nucleoplasm between clumps of bleached condensed chromatin. The clusters of interchromatin granules (IG) were only slightly labeled. When visible, nuclear bodies, including beaded bodies, coiled bodies, and IG-associated zones, were never labeled, and no labeling occurred on the nucleoli. The cytoplasm was not labeled with anti-hnRNP C, whereas Vav was detected on a few non-structured spots, mainly distributed within polyribosomes containing areas. The distribution of Vav in the nucleus and in the cytoplasm was estimated by counting the number of gold particles in both structures. From five different electron micrographs, we evaluated that 27% of cellular Vav was localized in the nucleus of UT7-S Epo cells. We did not detect gold particles by incubation of grids with normal rabbit serum or with normal mouse ascites fluid (both 1/10 in PBS) followed by immunogold labeling.
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Poly(U) RNA Increases the Vav-hnRNP C Interaction-- Previous studies have shown that hnRNP C binds to RNA molecules containing oligouridine stretches (18). Thus, to examine the physiological relevance of the Vav-hnRNP C interaction, we investigated the role of poly(U) RNA in this association. Coimmunoprecipitation experiments were performed with nuclear extracts from Jurkat or UT7-S Epo cells preincubated with increasing concentrations of poly(U) RNA (10-200 µg/ml) followed by the addition of anti-Vav antibodies. Blots were probed with anti-hnRNP C antibody and show that increasing amounts of poly(U) RNA enhanced the Vav-hnRNP C interaction (Fig. 6A) although similar amounts of Vav protein were immunoprecipitated (Fig. 6B). The opposite question, that of whether the total absence of RNA might impede the Vav-hnRNP C interaction, was also addressed. Coimmunoprecipitation experiments were performed as described above with nuclear extracts from UT7-S Epo cells either treated with or without RNase. Western blot analyses with anti-hnRNP C antibody revealed that a basal in vivo interaction was still detected in RNase treated extracts (Fig. 7, A and B). Similar results were obtained with nuclear extracts from Jurkat cells (data not shown). These findings indicate that, although Vav can bind to hnRNP C proteins that are either associated or free of poly(U) RNA, the higher affinity of Vav for hnRNP C-poly(U) RNA suggests that the folding of hnRNP C induced by poly(U) RNA is more favorable for an interaction with Vav.
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Role of RNA Homopolymers in Vav-hnRNP Interactions-- We next studied the Vav-hnRNP C association in the presence of nuclear RNA (hnRNA). hnRNA from UT7-S Epo cells was isolated and added to nuclear extracts from these cells. The amount of hnRNA used was similar to the amount found in the cells from which the nuclear extracts were isolated. After preincubation, extracts were immunoprecipitated with anti-Vav antibodies. Under these conditions, coimmunoprecipitated hnRNP C was detected, but at much lower levels than from nuclear extracts lacking hnRNA or with poly(U) RNA (Fig. 8, A and B). These data suggest that the presence of RNA molecules other than poly(U) RNA can decrease the Vav-hnRNP C interaction. This hypothesis was tested by analyzing the role of poly(U), poly(rC), poly(rG), and poly(rA) RNA as well as combinations of these RNA homopolymers with poly(U) RNA on Vav-hnRNP C binding. Coimmunoprecipitation experiments showed that both poly(rC) and poly(rG) RNA decreased the interaction of Vav with hnRNP C (6- and 2-fold, respectively), whereas poly(rA) RNA did not affect binding and, as expected, poly(U) RNA increased the association in a concentration-dependent manner (Fig. 9, A and B). The addition of poly(U) RNA to poly(rC) or poly(rG) RNA resulted in higher levels of associated hnRNP C as compared with poly(rC)- or poly(rG) RNA alone, but less than that observed with poly(U) RNA. In contrast, association in the presence of poly(rA·U)RNA and poly(U) RNA alone were similar. These results suggest that other RNA-binding proteins may play a role in the association of Vav with hnRNP C. To examine this possibility, we analyzed hnRNP K as a model of an RNA-binding protein with a preference for poly(rC) RNA. Anti-Vav immunoprecipitations were performed in the presence of RNA homopolymers or nuclear RNA and immunoblotted with an anti-hnRNP K monoclonal antibody (B4B6). As shown in Fig. 10 (A and B), the basal Vav-hnRNP K interaction was increased by poly(rC) RNA (5-fold), unchanged by poly(rA) RNA and decreased by the addition of poly(U) or poly(rG) RNA (5-fold each). Furthermore, the addition of nuclear RNA maintained the interaction, although at a low level. Overall, these findings suggest that the binding affinity of Vav for hnRNP proteins depends on the identity of associated RNA molecules. This opens new perspectives for the study of Vav interactions and functions.
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DISCUSSION |
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Vav is a complicated and interesting molecule because its structural features suggest that it may participate in several signaling pathways (5). Since it was first observed that Vav is rapidly tyrosine-phosphorylated on hematopoietic cells upon triggering of a large array of surface receptors (27, 40-42), several potential functions for Vav have been described (6, 7, 12, 14-17). Furthermore, in this report, we have demonstrated that Vav associates with hnRNP C proteins, suggesting a role for Vav as a hnRNP-interacting molecule. As hnRNPs are ubiquitous whereas vav is expressed solely in hematopoietic cells, Vav2 might replace Vav as an hnRNP partner in non-hematopoietic cells (3, 4).
In human cells, hnRNPs comprise a group of about 20 proteins that associate with pre-mRNA as RNA synthesis proceeds. Their functions are not fully understood, but they are thought to participate in posttranscriptional events such as regulated splicing and mRNA export (43). Although hnRNPs have a general affinity for RNA, the majority have distinct sequence preferences. For example, hnRNP C prefers poly(U) RNA whereas hnRNP K binds poly(rC) RNA (18). The initial model in which pre-mRNAs are packaged into a globular ribonucleoprotein structure, reminiscent of the way DNA is packaged in nucleosomes, contrasts with the current dynamic model. At present, there is evidence for the existence of transcript-specific complexes in which the protein composition varies during processing (43). The most abundant hnRNPs belong to groups A, B, and C, which are referred to as the core proteins. hnRNP C1 and C2 are identical, except for an insertion of 13 residues in the latter (35), and are restricted to the nucleus (36). The hnRNP C proteins have a RBD, acidic domain, NLS, NRS, putative NTP-binding site, and possible phosphorylation sites (18).
Through a two-hybrid screen in yeast, we detected a specific interaction of Vav with hnRNP C. This binding was confirmed in vitro using GST fusion proteins of both Vav and hnRNP C. The proline-rich motif present in the NRS of hnRNP C is involved in this interaction. In fact, a proline-rich peptide (YPARVPPPPPIARAV) contained in hnRNP C proteins or a proline to alanine mutant in this region were indeed capable of blocking the Vav-hnRNP C association. Using various subclones of Vav, we showed that the association with hnRNP C is mediated by the C-SH3 domain of Vav and that a P833L mutation in this region, which abolishes interactions with proline-rich motifs, completely eliminated the binding of Vav to hnRNP C. These data show that the interaction between Vav and hnRNP C is an SH3 domain interaction with a proline-rich sequence (39). Moreover, these findings raise the possibility that Vav participates in the mechanism of nuclear retention of hnRNP C, a hypothesis that remains to be tested.
Coimmunoprecipitation experiments and the nuclear localization of Vav and hnRNP C by electron microscopy suggest a functional role for Vav-hnRNP C association in hematopoietic cells. It was important to use non-transfected cells because ectopic expression of these genes, which would magnify the protein levels, might result in non-physiologically relevant results. In two hematopoietic cell lines (Jurkat and UT7-S Epo) using two different anti-Vav antibodies to immunoprecipitate the endogenous Vav, we were able to recover hnRNP C proteins and in the immunoprecipitate with anti-hnRNP C we detected Vav. These findings confirm the results found in vitro and in the yeast system. As described previously (36), we detected hnRNP C proteins only in the nuclear fraction of cells. Interestingly, electron microscopy of UT7-S Epo cells showed that both proteins localized on perichromatin fibrils (PF) and slightly on clusters of interchromatin granules (IG). The PF are formed at sites of transcription of pre-mRNA at the border of condensed chromatin and are the first extranucleolar structures to be labeled after short pulses with radioactive uridine (44, 45). After their formation, these fibrils migrate toward the interchromatin space while their RNA is being processed (46-48). A series of results using a variety of methodological approaches including parallel biochemical and morphological studies of purified subnuclear fractions (49, 50) led to the model that the PF correspond to structures containing newly synthesized pre-mRNA and to the main substrates of splicing. This conclusion is in agreement with the detection of small nuclear RNP antigens (51), small nuclear RNAs (52), and poly(A)+ RNAs (53) in association with PF. Cluster of IG have also been shown to contain high levels of spliceosomal components (51, 53). However, in contrast to PF, the IG were only slightly labeled with radioactive uridine, indicating that they were probably not active sites of transcription and splicing of pre-mRNA. Consequently, IG are thought to be involved in pre- or post-splicing events such as the assembly of spliceosomes, intron degradation, etc. (47, 52, 54). However, recently, small quantities of poly(A)+ RNA have been detected in some IG clusters (53), suggesting that IG might also be involved in some steps of intranuclear transport or sorting of mRNA molecules.
We and others have detected Vav in the nuclei (13-15, 17, 41). From our subcellular fractionations, we estimated that around 25% of cellular Vav was localized in the nuclei of UT7-S Epo cells (see Fig. 3A), which is similar to the amount calculated from the electron micrographs. Nevertheless, the colocalization experiments (Vav-hnRNP C) are probably meaningless due to the abundance of hnRNP C in the nuclei related to the amount of Vav.
As described above, RNA sequences that bind with high affinity to hnRNP C contain stretches of 5-14 uridines (55). We therefore analyzed the influence of poly(U) RNA on the Vav-hnRNP C association and found that the presence of oligouridine stretches significantly enhanced the interaction. Moreover, it was important to know whether the Vav-hnRNP C interaction was still maintained in the absence of RNA, because RNA strands might be responsible for the detected association (18). Coimmunoprecipitation of Vav and hnRNP C were obtained from nuclear extracts treated with RNase, indicating that the Vav-hnRNP C interaction is not mediated by RNA strands. These results agree with the SHVAV-v90 interaction in absence of the RNA-binding domain in the v90 subclone. Although Vav and RNA bind to separate sites within the hnRNP C molecule, it is tempting to speculate that the specific configuration of the proline-rich motif (in the NRS) changes upon binding of poly(U) RNA to the RBD motif of hnRNP C, hence allowing increased interaction with Vav. In HeLa cells, a dynamic cycle of hnRNP C phosphorylation/dephosphorylation takes place, regulating its binding to pre-mRNA. Indeed, hyperphosphorylated hnRNP C proteins do not bind to pre-mRNA (20). As the hnRNP C NRS also contains potential phosphorylation sites for casein kinase II and protein kinase C (36), it is possible that phosphorylation may negatively regulate the Vav-hnRNP C interaction. Thus, hyperphosphorylation of hnRNP C might abolish its binding to pre-mRNA and Vav.
Furthermore, these studies have shown that different RNA homopolymers modify the Vav-hnRNP C association. Vav binds strongly to hnRNP C if poly(U) RNA is added, but this interaction decreases in the presence of poly(rC) RNA. In contrast, the addition of poly(rC) RNA increases the binding of Vav to hnRNP K, probably because the poly(rC) RNA-hnRNP K complex has a structure that allows a better association with Vav. In addition, both poly(rC) and poly(rG) RNA decrease the basal Vav-hnRNP C interaction, indicating that other RNA-binding proteins might also bind to Vav. hnRNPs E, F and H bind to poly(rG) RNA, and hnRNPs J and K prefer poly(rC) RNA (18). It is tempting to postulate that at least some of these proteins might interact with Vav. In addition, Vav also localizes in polyribosome-containing areas, supporting this hypothesis. More importantly, we have shown that Vav-hnRNP C interactions were maintained in the presence of native nuclear RNA implying a biological relevance of these associations.
The role of hnRNPs in splicing derives from the preferential affinity of these proteins for splicing signals and from the activity of several hnRNPs in splice-site selection. It seems that the relative levels of hnRNPs and other splicing factors can influence alternative splicing modes, affecting the expression of a wide variety of genes (56, 57). Proteins other than hnRNPs may be involved in the configuration of perichromatin fibrils, after specific RNA-protein and protein-protein interactions. These proteins would present the substrate and recruiting splicing factors to selected sites as well as for mRNA export. We propose that Vav might participate in a cycle of interactions with hnRNP proteins from pre-mRNA synthesis to mRNA export. This putative role for Vav would depend on the composition of RNA molecules and the identity and phosphorylation state of associated hnRNPs.
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ACKNOWLEDGEMENTS |
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We thank A. Altman, M. Barbacid, X. Bustelo, J. Celis, K. Dejgaard, G. Dreyfuss, O. Hobert, W. H. Reeves, and A. Ullrich for their generous contribution of reagents, without which these studies could not have been performed. We also thank N. Taylor for critical reading of the manuscript, and F. Pozo and A. Viron for technical assistance.
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FOOTNOTES |
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* This work was supported in part by INSERM and Ligue Nationale Contre le Cancer.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
§ Supported by postdoctoral fellowships from the EEC and Fondation pour la Recherche Médicale. To whom correspondence should be addressed. Tel.: 33-140469332; Fax: 33-146339297; E-mail: romero{at}icgm.cochin.inserm.fr.
1 The abbreviations used are: SH, Src homology domain; C-SH, carboxyl-SH; hnRNP, heterogeneous nuclear ribonucleoprotein; PBS, phosphate-buffered saline; GST, glutathione S-transferase; PAGE, polyacrylamide gel electrophoresis; RBD, RNA-binding domain; NLS, nuclear localization signal; NRS, nuclear retention sequence; IG, interchromatin granule(s); PF, perichromatin fibril(s).
2 A. Altman, unpublished data.
3 F. Romero, A. Germani, E. Puvion, J. Camonis, N. Varin-Blank, S. Gisselbrecht, and S. Fischer, unpublished work.
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