From the Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565, Japan
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ABSTRACT |
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We have recently isolated SMAP (Smg GDS-associated protein; Smg GDS: small G protein GDP dissociation stimulator) as a novel Smg GDS-associated protein, which has Armadillo repeats and is phosphorylated by Src tyrosine kinase. SMAP is a human counterpart of mouse KAP3 (kinesin superfamily-associated protein) that is associated with mouse KIF3A/B (a kinesin superfamily protein), which functions as a microtubule-based ATPase motor for organelle transport. We isolated here a SMAP-interacting protein from a human brain cDNA library, identified it to be a human homolog of Xenopus XCAP-E (Xenopus chromosome-associated polypeptide), a subunit of condensins that regulate the assembly and structural maintenance of mitotic chromosomes, and named it HCAP (Human chromosome-associated polypeptide). Tissue and subcellular distribution analyses indicated that HCAP was ubiquitously expressed and highly concentrated in the nuclear fraction, where SMAP and KIF3B were also present. SMAP was extracted as a ternary complex with HCAP and KIF3B from the nuclear fraction in the presence of Mg-ATP. The results suggest that SMAP/KAP3 serves as a linker between HCAP and KIF3A/B in the nucleus, and that SMAP/KAP3 plays a role in the interaction of chromosomes with an ATPase motor protein.
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INTRODUCTION |
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We have isolated SMAP (Smg GDS-associated protein; Smg GDS: small G protein GDP dissociation stimulator) as a novel Smg GDS-associated protein, which has Armadillo repeats and is tyrosine-phosphorylated by Src tyrosine kinase (1). SMAP is a human counterpart of mouse KAP3 (kinesin superfamily-associated protein) that forms a complex with mouse KIF3A/B (a kinesin superfamily protein) (2). KIF3A and KIF3B form a heterodimer that functions as a microtubule-based fast anterograde translocator of membranous organelles (3). The head domain of KIF3A/B, containing the ATPase activity, binds to a microtubule and the tail domain binds to KAP3 (2, 3). Smg GDS is a regulator that stimulates the GDP/GTP exchange reaction of a group of small G proteins, including the Rho and Rap1 family members and Ki-Ras (4, 5). The interaction of SMAP with Smg GDS is regulated by the phosphorylation of SMAP by v-Src kinase (1). It is suggested that SMAP/KAP3 binds to the cargo and regulates the binding of KIF3A/B to its cargo and that SMAP/KAP3 links KIF3A/B to the Smg GDS-regulated small G proteins and Src kinase signalings. However, the precise function of SMAP/KAP3 remains unknown. One way to further clarify the mode of action of SMAP/KAP3 is to isolate a SMAP/KAP3-interacting protein(s).
We attempted here to isolate a SMAP-interacting protein from a human B-cell cDNA library by use of the yeast two-hybrid method, identified it to be a human homolog of Xenopus XCAP-E (Xenopus chromosome-associated polypeptide), and named it HCAP (Human chromosome-associated polypeptide). XCAP-E is an SMC (stability of minichromosomes) family member (6). The SMC family members are found in a range of organisms from yeast to mammal and required for the chromosome dynamics, including chromosome condensation, chromosome segregation, and X chromosome dosage compensation (for reviews, see Refs. 7 and 8). For instance, Xenopus XCAP-C and -E are required for both the assembly and structural maintenance of mitotic chromosomes (6), and mutants of SMC1 and SMC2 in Saccharomyces cerevisiae are defective in proper segregation of mitotic chromosomes (9). Our results suggest that SMAP/KAP3 plays a role in the interaction of chromosomes with an ATPase motor protein.
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EXPERIMENTAL PROCEDURES |
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Materials and Chemicals--
The
MBP1 fusion proteins of SMAP
and the C-terminal fragment of HCAP (865-1217 aa) (MBP-SMAP and
MBP-HCAP, respectively) and the GST fusion proteins of the C-terminal
fragments of HCAP (865-1217 aa) and HA-tagged KIF3B (593-747 aa)
(GST-HCAP and GST-HA-KIF3B, respectively) were purified from
Escherichia coli overexpressing each protein (1). A rabbit
anti-HCAP polyclonal antibody was generated against MBP-HCAP, purified
by a MBP-HCAP-coupled affinity column, and further purified by passage
over a MBP-coupled affinity column to be separated from an anti-MBP
polyclonal antibody. The cDNA fragment encoding the C-terminal
region of mouse KIF3B (593-747 aa) was cloned from a mouse brain
cDNA library (CLONTECH) using a polymerase
chain reaction (10). Anti-KIF3B polyclonal and anti-lamin A/C
monoclonal antibodies were kindly provided by N. Hirokawa (Tokyo
University, Tokyo, Japan) and Y. Matsuoka (Osaka University, Osaka,
Japan), respectively. An anti-1/
2-adaptin monoclonal antibody was purchased from Sigma.
Isolation and Cloning of HCAP cDNA--
pLexA-SMAP was
obtained by inserting the cDNA fragment encoding the N-terminal
region of SMAP (1-562 aa) into the polylinker site of pBTM116-HA (1)
and used as a yeast two-hybrid bait vector. Yeast two-hybrid screens of
a human B-cell cDNA library in pACTII
(CLONTECH) were performed and evaluated as
described (1). The positive clone encoding the C-terminal region of
HCAP (865-1217 aa) was isolated. A human brain cDNA library in
ZAPII (Stratagene) was screened to determine the full-length
sequence using the positive clone as a probe. Multiple overlapping
clones covering the entire coding region were sequenced to assemble the full-length sequence. The HCAP cDNA sequence reported in this work
is based on the determination of cDNA sequences of both strands of
the HCAP cDNA.
Assay for Direct Interactions of SMAP with HCAP-- GST-HCAP or GST (0.1 nmol) was mixed with MBP-SMAP or MBP (0.1 nmol) in 0.2 ml of Buffer A (25 mM Tris/HCl at pH 7.5, 0.5 mM EDTA, and 1 mM DTT) and incubated for 2 h at 4 °C. After the incubation, each mixture was applied to a glutathione-Sepharose column (0.1 ml) equilibrated with Buffer A. After the column was washed with Buffer A, the proteins bound to GST-HCAP or GST were coeluted by 0.3 ml of Buffer A containing 20 mM glutathione. Each eluate was subjected to SDS-PAGE, followed by protein staining with Coomassie Brilliant Blue.
Subcellular Fractionation of COS-7 Cells-- Subcellular fractionation of COS-7 cells was performed as described (11). Briefly, COS-7 cells grown in 10-cm dishes (1) were rinsed twice with cold phosphate-buffered saline and harvested by scraping. All manipulations were performed at 0-4 °C. Cells were pipetted in 7 volumes of homogenizing buffer (10 mM HEPES/KOH at pH 7.3, 10 mM KCl, 5 mM MgCl2, 0.5 mM DTT, 3 mg/liter cytochalasin B, 0.2 mM PMSF, and 10 mg/liter leupeptin) and incubated for 30 min. They were homogenized in a Potter-Elvehjem Teflon-glass homogenizer and centrifuged at 1,000 × g for 10 min. The supernatant was used as the cytoplasmic fraction. The pellet was resuspended in 10 volumes of Buffer B (10 mM triethanolamine/HCl at pH 7.5, 5 mM MgCl2, 0.2 mM PMSF, and 10 mg/liter leupeptin) containing 1.62 M sucrose, layered on the top of a 2.3 M sucrose cushion, and centrifuged at 160,000 × g for 1 h. The pellet was used as the nuclear fraction.
Immunostaining of COS-7 Cells-- The cells were fixed and stained with the anti-HCAP antibody (4 µg/ml protein) or preimmune rabbit IgG (4 µg/ml protein) as described (12). Fluorescein-conjugated donkey anti-rabbit IgG (Chemicon) was used as the second antibody. Chromosomes were stained with DAPI (Sigma). The stained cells were observed with a Zeiss Axiophoto microscope (Carl Zeiss, Oberkochen, Germany) and photographed with a peltier cooling 3CCD color camera (C5810-01; Hamamatsu Photonics KK., Hamamatsu, Japan).
Coimmunoprecipitation of SMAP and KIF3B with HCAP-- All manipulations were performed at 0-4 °C. The nuclear fraction of COS-7 cells (2.4 mg of protein) was resuspended with 0.8 ml of Buffer B containing 5 mM ATP, sonicated, and incubated for 1 h. After centrifugation at 60,000 × g for 20 min, the supernatant was used as the Mg-ATP extract. The pellet was resuspended with 0.8 ml of Buffer B. Twenty percent Nonidet P-40 was added to the Mg-ATP extract, giving a final concentration of 1%, and the mixture was immunoprecipitated with the anti-HCAP antibody, followed by Western blotting using the indicated antibodies as described (1).
Other Procedures-- SMAP, HCAP, and KIF3B transferred to nitrocellulose sheets after SDS-PAGE were detected using the ECL immunoblotting detection system (Amersham Pharmacia Biotech). The amount of each protein was determined by densitometric tracing at 420 nm with each purified protein as a standard in a linear range as described (13).
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RESULTS AND DISCUSSION |
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We first attempted to isolate a SMAP-interacting protein from a human B-cell cDNA library by the yeast two-hybrid method with SMAP as bait. Among 4 × 105 total transformants, nine clones were positive for the screening. DNA sequencing of the insert DNAs of these clones revealed that three clones (1.4 kilobase pairs) were identical and encoded a novel amino acid sequence, which was homologous to Xenopus XCAP-E, an SMC family member (6). Since all the isolated clones were truncated forms, we next isolated the full-length cDNA clone from a human brain cDNA library using the positive clone as a probe and determined its nucleotide sequence. The encoded protein consisted of 1,217 aa with a calculated Mr of 141,540 and was named HCAP (accession number AF020043) (Fig. 1A). The neighboring sequence of the first ATG was consistent with the translation initiation start site proposed by Kozak (14), and in-frame stop codons were present upstream of the first ATG. Moreover, the position of the in vitro translated product of full-length HCAP was similar to that of endogenous HCAP on SDS-PAGE (data not shown). We have concluded from these results that the isolated clone contains the open reading frame of HCAP. An initial data base search demonstrated that HCAP shared 53, 41, and 22% overall amino acid sequence identities with Drosophila DCAP (15), Aspergillus SUDA (16), and Xenopus XCAP-E, respectively. DCAP and SUDA are also SMC family members (15, 16). HCAP had a head-rod-tail structural organization, which the SMC family members commonly have (Fig. 1B) (7, 8). The head contained an NTP-binding motif and the tail contained a DA-box. These results indicate that HCAP is an SMC family member. Recently, the full sequence of the rat basement membrane-chondroitin sulfate proteoglycan was directly submitted to GenBankTM (accession number U82626). The cDNA of this protein was isolated by screening the cDNA expression library with polyclonal antiserum raised against basement membrane proteoglycans, and the properties of this protein were not reported (17), but this protein shared 98% amino acid sequence identity with HCAP.
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We confirmed using the yeast two-hybrid method that the isolated HCAP indeed interacted with SMAP (Fig. 2A). In these experiments, the N-terminal two-thirds of SMAP (1-562 aa) and the C-terminal one-third of HCAP (865-1217 aa) were used. Ras(G12V) and Raf, known to interact in the yeast two-hybrid method (18), were used as positive controls. Moreover, we examined using the recombinant samples whether HCAP directly interacts with SMAP in a cell-free system. MBP-SMAP bound to GST-HCAP, but not to GST (Fig. 2B). MBP did not bind to GST-HCAP (data not shown). These results indicate that SMAP directly interacts with HCAP.
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Western blot analysis using the anti-HCAP antibody detected only an ~140-kDa protein in all the rat tissues examined and the molecular mass was similar to a Mr value calculated from the open reading frame (Fig. 3A). The subcellular distribution of HCAP in intact COS-7 cells indicated that HCAP was highly concentrated in the nuclear fraction and hardly detected in the cytoplasmic fraction (Fig. 3B). Both SMAP and KIF3B were detected in both the nuclear and cytoplasmic fractions. Immunostaining of COS-7 cells with the anti-HCAP antibody showed that HCAP was localized on mitotic chromosomes (Fig. 3C). Chromosomes were not stained with preimmune rabbit IgG (data not shown). These results suggest that SMAP forms a complex with HCAP and/or KIF3A/B in the nucleus.
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We, therefore, examined whether SMAP interacts with HCAP and/or KIF3A/B in the nuclear fraction. It has been shown that hydrolysis of ATP disrupts the interaction of an ATPase protein(s) with other protein(s): myosin I ATPase is extracted from an actin filament in the presence of Mg-ATP (19); and NSF ATPase is extracted from a complex of SNAPs and SNAP receptors in the presence of Mg-ATP (20). Moreover, it has recently been reported that 13 S condensin, a complex containing XCAP-C and -E, has a DNA-stimulated ATPase activity and that the XCAP-C and -E subunits are indispensable for this activity (21). These observations suggest that HCAP and/or KIF3A/B should be extracted with SMAP from the nuclear fraction in the presence of Mg-ATP. When the nuclear fraction of COS-7 cells was incubated in the presence of Mg-ATP, SMAP, HCAP, and KIF3B were extracted (Fig. 4A). None of these proteins was extracted in the absence of Mg-ATP. Most of SMAP and KIF3B, but only a small amount of HCAP, were extracted. Furthermore, SMAP and KIF3B were coimmunoprecipitated with HCAP with the anti-HCAP antibody from the Mg-ATP extract (Fig. 4B). These results suggest that SMAP forms a ternary complex with HCAP and KIF3A/B in the nucleus and that hydrolysis of ATP by HCAP and/or KIF3A/B disrupts their interactions with other proteins. The reason why only a small amount of HCAP was extracted is not known, but since it was estimated by Western blotting that in the nuclear fraction, the amount of HCAP was about 10-fold more than that of SMAP, only the SMAP·HCAP·KIF3A/B complex might be extracted.
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Our present results indicate that SMAP forms a ternary complex with
KIF3B and HCAP, a human SMC family member, and suggest that this
ternary complex is associated with unknown proteins in the nucleus.
XCAP-E forms at least two major complexes, 8 and 13 S condensins, and 8 S condensin contains two SMC family members, XCAP-C and -E, whereas 13 S condensin contains three subunits, XCAP-D2, -G, and -H, in addition
to XCAP-C and -E (22). 13 S condensin is required for chromosome
condensation, but the function of 8 S condensin remains unknown (22).
Moreover, a recombination protein complex (RC-1) purified from calf
thymus contains two SMC family members, the 160- and 130-kDa
polypeptides, and two additional subunits, DNA polymerase and DNA
ligase III (23). The 160- and 130-kDa polypeptides alone exhibit a DNA
reannealing activity, whereas RC-1 has the activity to catalyze
recombinational repair of double strand gaps and deletions in DNA in
addition to the DNA reannealing activity (23). It is likely that an SMC family member interacts with different sets of subunits, thereby modifying and acquiring its unique functions. The SMAP·HCAP·KIF3A/B complex might have a novel function, different from those thus far
reported in the SMC family members.
We have shown here that HCAP is associated with mitotic chromosomes as described for XCAP-E (6, 22). Sea urchin kinesin II is associated with pericentriolar and intranuclear regions during prophase, with kinetochore-to-pole microtubules near the kinetochores during metaphase (24), suggesting that a mammalian counterpart, KIF3A/B, also binds to the spindle and plays a role in mitosis. Taken together, it is likely that HCAP forms a complex with SMAP and KIF3A/B in the interphase nucleus and that during mitosis the complex tethers chromosomes to the spindle and plays a role in chromosome movement, like the microtubule-dependent motor CENP-E (25). Further study is necessary to clarify the function of the SMAP·HCAP·KIF3A/B complex in the nucleus.
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ACKNOWLEDGEMENT |
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We are grateful to Drs. Yoshihiro Yoneda and Taro Tachibana (Osaka University Medical School) for helpful discussions.
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FOOTNOTES |
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* This work was supported by grants-in-aid for Scientific Research and for Cancer Research from the Ministry of Education, Science, Sports, and Culture, Japan (1996, 1997), by grants-in-aid for Abnormalities in Hormone Receptor Mechanisms and for Aging and Health from the Ministry of Health and Welfare, Japan (1996, 1997), and by grants from the Human Frontier Science Program (1996, 1997) and the Uehara Memorial Foundation (1996).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF020043.
To whom correspondence should be addressed: Dept. of Molecular
Biology and Biochemistry, Osaka University Medical School, 2-2 Yamada-oka, Suita 565, Japan. Tel.: 81-6-879-3410; Fax: 81-6-879-3419; E-mail: ytakai{at}molbio.med.osaka-u.ac.jp.
1 The abbreviations used are: MBP, maltose-binding protein; GST, glutathione S-transferase; aa, amino acids; HA, influenza hemagglutinin; DTT, dithiothreitol; PAGE, polyacrylamide gel electrophoresis; PMSF, phenylmethylsulfonyl fluoride; DAPI, 4',6'-diamidino-2-phenylindole.
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REFERENCES |
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