From the Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138
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ABSTRACT |
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Alanine substitution mutagenesis of Escherichia coli DNA topoisomerase I, a member of the type IA subfamily of DNA topoisomerases, was carried out to identify amino acid side chains that are involved in transesterification between DNA and the active site tyrosine Tyr-319 of the enzyme. Twelve polar residues that are highly conserved among the type IA enzymes, Glu-9, His-33, Asp-111, Glu-115, Gln-309, Glu-313, Thr-318, Arg-321, Thr-322, Asp-323, His-365, and Thr-496, were selected for alanine substitution. Each of the mutant enzymes was overexpressed, purified, and characterized. Surprisingly, only substitution at Glu-9 and Arg-321 was found to reduce the DNA relaxation activity of the enzyme to an insignificant level. The R321A mutant enzyme, but not the E9A mutant enzyme, was found to retain a reduced level of DNA cleavage activity. Two additional mutant enzymes R321K and E9Q were also constructed and purified. Replacing Arg-321 by lysine has little effect on enzymatic activities; replacing Glu-9 by glutamine greatly reduces the supercoil removal activity but not the DNA cleavage and rejoining activities. From these results and the locations of the amino acids in the crystal structure of the enzyme, it appears that Glu-9 has a critical role in DNA breakage and rejoining, probably through its interaction with the 3' deoxyribosyl oxygen. The positively charged Arg-321 may also participate in these reactions by interacting with the scissile DNA phosphate as a monodentate. Because of the strict conservation of these residues, the findings for the E. coli enzyme are likely to apply to all type IA DNA topoisomerases.
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INTRODUCTION |
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DNA topoisomerases are enzymes that participate in nearly all cellular transactions of DNA, including replication, transcription, and chromosome condensation (for reviews see Ref. 1 and references therein). There are two types of DNA topoisomerases: the type I enzymes catalyze the transport of individual DNA strands through one another and the type II enzymes catalyze the interpenetration of double-stranded DNA segments. By doing so, the DNA topoisomerases alleviate the topological problems encountered by intracellular DNA.
The transport of DNA strands through one another requires the transient breakage of one of the encountering pair, and all DNA topoisomerases catalyze this reaction through the formation of covalent enzyme-DNA intermediates; the phenolic oxygen of an enzyme tyrosyl residue undergoes nucleophilic attack of a DNA phosphorous to break a DNA phosphodiester bond and form a phosphotyrosine link (2, 3). Following DNA strand breakage and passage, the deoxyribosyl hydroxyl formed during DNA breakage acts as the nucleophile to break the phosphotyrosine link and rejoin the DNA strand.
By analogy to the cleavage of DNA by nucleases (4) and from studies of the pH dependences of the reaction steps catalyzed by vaccinia virus topoisomerase (5, 6), it was suggested that transesterification mediated by the DNA topoisomerases might involve general acid-base catalysis. In the formation of the enzyme-DNA covalent adduct, a general base in the enzyme might assist in the removal of the hydroxyl proton of the active site tyrosine, and a separate general acid in the enzyme might assist in the protonation of the departing deoxyribosyl oxygen. The DNA rejoining reaction after strand passage could be the exact microscopic reversal of the DNA breakage reaction (5-7), but there has been no definitive experimental test of this conjecture.
In this work, we report a mutational analysis of Escherichia coli DNA topoisomerase I for the identification of amino acid side chains that might be directly involved in transesterification between Tyr-319 of the enzyme and the DNA scissile phosphorus (4). The E. coli enzyme is representative of a subfamily of type I DNA topoisomerases, the type IA enzymes, from a diverse collection of organisms including bacteria, eukarea, and archaea (1, 8). Alignment of amino acid sequences of these enzymes showed a large number of highly conserved amino acid residues (9). This subfamily of enzymes are very different, however, from the type IB DNA topoisomerases, whose members include eukaryotic DNA topoisomerase I and vaccinia virus topoisomerase, both in amino acid sequences and reaction mechanisms (1). Several studies on the identification of active site residues in vaccinia virus DNA topoisomerase have been reported recently (10-13).
E. coli DNA topoisomerase I is encoded by the topA gene comprised of 865 codons (14). For convenience, the positions of all amino acid residues in the 97-kDa single polypeptide protein are referred to by their codon numbers, even though the N-terminal methionine is removed post-translation (14). The three-dimensional structure of a 67-kDa fragment of the enzyme has been determined by x-ray crystallography (15), and that of a C-terminal fragment comprised of amino acids 745-865 has been determined by nuclear magnetic resonance (16). The latter fragment is dispensable for catalytic activity but appears to participate in substrate binding (17, 18). The polypeptide bridging these two fragments contains three motifs with four cysteines in each. These tetracysteine motifs are most likely the binding sites of three Zn(II) ions (19).
The 67-kDa N-terminal fragment is capable of covalent adduct formation with single-stranded DNA (15). Therefore, it is most likely to contain all residues that are essential for covalent catalysis. In the crystal structure of this fragment, the polypeptide is folded into four distinct domains (15). The fragment can be viewed to comprise a "base" formed by domains I and IV and a "lid" formed by domains II and III (see Fig. 1A). In the crystal, the base and the lid are touching on one side through contacts between domain III in the lid and domains I and IV in the base and are linked on the other side by a pair of long strands between domains II and IV. The four domains and the pair of connecting strands enclose a 28 Å hole. The active site tyrosine has been identified to be Tyr-319 (4). It is located in domain III at the interface between this domain and domains I and IV. This strategic position suggests that during the catalysis of DNA breakage, passage, and rejoining, domain III on one side and domains I and IV on the other are likely to undergo large relative movements (15). Following the formation of the covalent adduct, for example, the lid is probably lifted away from the base to allow the passage of a second strand (15). The structural features of the 67-kDa fragment and the homology alignment of the amino acid sequences of the type IA enzymes provided a useful backdrop for the studies described below.
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EXPERIMENTAL PROCEDURES |
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Site-directed Mutagenesis--
Alanine substitution mutants were
constructed by site-directed mutagenesis, using a commercial kit and
following the protocol of the supplier (CLONTECH).
The E. coli DNA topoisomerase I overexpression plasmid
pJW312 (16) was used in these constructions. A selection primer, which
changes a unique ScaI site within the -lactamase gene of
the plasmid to a SalI site, and mutagenesis primers for the
intended alanine codon substitutions, were purchased from commercial
suppliers. The nucleotide sequences of the mutagenesis primers used in
the construction of the E9A, H33A, D111A, E115A, Q309A, E313A,
T318A, Y319A, R321A, T322A, D323A, H365A, and T496A were, respectively,
5'-CTT-GTC-ATC-GTT-GCT-AGC-CCG-GCA-AAA-GCC, 5'-TCC-AGC-GTC-GGC-GCC-ATC-CGC-GAT-TTG-C,
5'-C-ATC-TAT-CTC-GCG-ACC-GCC-CTT-GAC-CGC-G, 5'-C-CTT-GAC-CGC-GCC-GGC-GAA-GCC-ATT-GCA-TG,
5'-CC-ATG-ATG-ATG-GCG-GCG-CGC-TTG-TAT-GAA-GCA-GGC, 5'-CAG-CGT-TTG-TAT-GCA-GCC-GGC-TAT-ATC-ACT-TAC,
5'-GCA-GGC-TAT-ATC-GCT-TAC-ATG-CGC-ACC-GAC-TCC-AC, 5'-CA-GGC-TAT-ATC-ACT-GCC-ATG-CGC-ACC-GAC-TCC-AC,
5'-C-TAT-ATC-ACT-TAC-ATG-GCC-ACC-GAC-TCC-AC, 5'-C-ACT-TAC-ATG-CGC-GCC-GAC-TCC-ACT-AAC,
5'-CT-TAC-ATG-CGT-ACG-GCC-TCC-ACT-AAC-CTG, 5'-C-TCA-CAG-GCA-GCG-GCC-GAA-GCG-ATT-CGC,
5'-CA-GAT-GCG-CAG-AAG-CTA-GCC-CAG-TTA-ATC-TGG-C, and
5'-GT-CGT-CCG-TCT-GCA-TAT-GCG-TCG-ATC (for clarity, hyphens are inserted in between codons). The oligonucleotides used in the
construction of E9Q and R321K were
5'-CTT-GTC-ATA-GTT-CAG-TCG-CCG-GCA-AAA-3' and
5'-ATC-ACT-TAC-ATG-AAG-ACC-GAC-TCC-ACT-3', respectively. In the
design of each mutagenesis primer, silent mutations were often included
to introduce a restriction site (underlined hexameric sequences in the
mutagenesis primers specified above), so that the presence of the
intended mutation could be checked by digestion of the mutated plasmid
with the particular restriction enzyme. In the construction of R321K, a
RsaI site in the wild-type topA gene was
eliminated for the same purpose. Further confirmation of the presence
of the intended mutation in each of the mutant plasmid was carried out
by DNA sequencing.
Overexpression and Purification of Mutant Proteins--
pJW312
or its mutated derivative, which expresses wild-type or mutant E. coli DNA topoisomerase I from an inducible lac promoter (4), was
transformed into E. coli BL21 topA+
or DM800 topA cells bearing a pACYC184-based
lacI clone. Induction of cells for overexpression of the
topoisomerase by the addition of
isopropyl-1-thio-
-D-galactoside and lysis of the cells
with lysozyme and the nonionic detergent Brij-58 were carried out as described previously for the preparation of wild-type E. coli DNA topoisomerase I (4). Following the removal of cell debris by centrifugation, the lysate was directly loaded on a phosphocellulose column and eluted as described (4). Peak fractions were pooled and
further purified by high pressure liquid chromatography, using an SP
column (Bio-Rad). Purity of each protein was examined by SDS-polyacrylamide gel electrophoresis. Expression levels of all mutants were found to be comparable with that of wild-type E. coli DNA topoisomerase I. Higher yields were generally achieved in
preparations from BL21 cells than DM800 cells, but preparations from
DM800 cells were used in the relaxation assays owing to the possibility
of contaminating wild-type DNA topoisomerase I in preparations from
BL21 cells.
DNA Cleavage Assays--
Cleavage of uniquely end-labeled DNA
was carried out in a buffer containing 40 mM Tris·HCl, pH
7.5, and 10 mM KCl. In some experiments, EDTA was also
present at 0.1 mM. A 388-base pair long
EcoRI-NcoI restriction fragment was used in the
cleavage assays. The plasmid pJW312 containing the fragment was first
cut with EcoRI, and the 5' ends were 32P-labeled
by a cycle of dephosphorylation with calf intestine alkaline
phosphatase and phosphorylation with T4 polynucleotide kinase in the
presence of [-32P]ATP. After phenol extraction and
ethanol precipitation in the presence of ammonium acetate to remove the
unincorporated triphosphate, the labeled DNA was resuspended and
digested with NcoI, and the DNA fragment labeled only at its
EcoRI end was purified by electrophoresis in a 1.5% agarose
gel. This uniquely end-labeled DNA was heat denatured and used in the
cleavage assays. Further details of the DNA relaxation and cleavage
assays are described in the legends to Figs. 2 and 3.
Reversal of DNA Cleavage by Addition of Salt-- Reversal of DNA cleavage by the addition of NaCl to 0.8 M or NaCl and MgCl2 to 0.8 M and 10 mM, respectively, was carried out to test whether a mutant enzyme capable of cleaving single-stranded DNA could rejoin the cleaved DNA (21, 22). Following incubation of the enzyme and the 5' end-labeled DNA fragment, each sample was split into three equal volume portions. One of each triplicate was used for measuring DNA cleavage by the addition of SDS to 1%. For the remaining two, MgCl2 was added to one to a final concentration of 10 mM, and NaCl was added to both to a final concentration of 0.8 M. The pair of samples were incubated at 37 °C for an additional 30 min before the addition of SDS. Following the removal of salt by ethanol precipitation, the samples were resuspended in water for electrophoresis in a 6% polyacrylamide DNA sequencing gel.
Oligonucleotide Cleavage and Rejoining-- A gel-purified nonamer 5'-CAATGCGCT-3', 32P-labeled at its 5' end, was used as the substrate. The concentrations of E. coli DNA topoisomerase I and the oligonucleotide were approximately 0.4 and 1 µM, respectively. Ethanol precipitation of the radiolabeled oligomer was done in the presence of glycogen (Boehringer Mannheim).
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RESULTS |
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Mutagenesis of Highly Conserved Amino Acid Residues in E. coli DNA Topoisomerase I-- To identify amino acid side chains of E. coli DNA topoisomerase I that might be directly involved in the catalysis of DNA breakage and rejoining, 12 point mutants, E9A, H33A, D111A, E115A, Q309A, E313A, T318A, R321A, T322A, D323A, H365A, and T496A, each designated by the particular amino acid residue replaced by alanine, were constructed by site-directed mutagenesis. An additional mutant Y319A was also constructed for comparison with the others, because inactivation of the enzyme by this mutation was anticipated from the known function of Tyr-319 in catalysis (4), as well as from previous studies of the Y319F and Y319S mutant enzymes (23).
In selecting the residues for alanine substitution, it was assumed that a particular catalytic residue must be present at corresponding positions in all members of this subfamily and that it must possess a polar side chain, which is likely to be involved in the catalysis of DNA breakage and rejoining. Variability in residues at a conserved position was deemed acceptable only if all residues at the position contained a similar chemical group: interchanges among aspartate, glutamate, asparagine, and glutamine at a particular position in the homology alignment, for example, were considered acceptable because of the presence of a carbonyl group in each of the residues; on the other hand, a highly conserved amino acid residue such as Tyr-312 was considered to be an unlikely candidate because of the presence of a phenylalanine at this position in Sulfolobus acidocaldarius reverse gyrase (9). Several amino acid residues, including Lys-90, Arg-209, Tyr-391, and Glu-547, which fulfill the criterion of being functionally conserved, were not selected for mutagenesis because of their relatively distal locations from the active site tyrosine Tyr-319 (15). The locations of Tyr-319 and the other 12 amino acid residues selected for alanine substitution mutagenesis are shown in Fig. 1B.
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Relaxation of Negatively Supercoiled DNA by Wild-type and Mutant
Enzymes--
Wild-type E. coli DNA topoisomerase I and the
13 alanine substitution mutant proteins were individually overexpressed
in topA cells harboring plasmids encoding the proteins.
All mutant proteins were found to be expressed to a high level
comparable with that of the wild-type enzyme. Each of the overexpressed
proteins was purified to homogeneity and assayed for its ability to
relax negatively supercoiled DNA. In Fig.
2, each set of four lanes represents assays in which the concentration of the topoisomerase was successively diluted 5-fold each time from left to right.
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Formation of Covalent Adduct between Mutant E. coli DNA
Topoisomerase I and DNA--
Fig. 3
illustrates the results of a typical experiment in which covalent
adduct formation between DNA and wild type (lane 1), E9A
(lane 2), E9Q (lane 3), Y319A (lane
4), and R321A mutant enzyme (lane 5) was examined. A
388-base pair-long DNA fragment uniquely labeled at a 5' end was used
in this experiment. The fragment was heat denatured and incubated with
wild-type or mutant E. coli DNA topoisomerase I in 40 mM Tris·HCl, pH 7.5, 10 mM KCl, and SDS was
then added to 1%. As shown previously, protein-DNA covalent adduct
formation is accompanied by cleavage of single-stranded DNA at sites
that are determined by both structural and sequence features (24, 25).
There is a strong preference of a cytosine at position 4, that is,
four nucleotides upstream of the cleavage site (2). In the case of
wild-type E. coli DNA topoisomerase I (lane 1 of
Fig. 3), the specificity of the cleavage reaction resulted in a
distinctive distribution of cleavage products (compare the pattern of
the lane 1 sample with that of the untreated control run in
lane 6). For the two mutant proteins E9A and R321A that showed little DNA relaxation activity (Fig. 2), the former showed no
cleavage activity (lane 2 of Fig. 3), whereas the latter
showed reduced but significant level of DNA cleavage (lane 5 of Fig. 3). In contrast to the E9A mutant, the E9Q mutant protein
showed full cleavage activity (lane 3 of Fig. 3). In some
cleavage assays, the reaction mixtures also contained 0.1 mM EDTA; no difference in the cleavage patterns was
observed by the inclusion of this metal chelating agent.
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DNA Rejoining by Mutant E. coli DNA Topoisomerase I-- For the complex between single-stranded or negatively supercoiled DNA and bacterial DNA topoisomerase I, it is known that addition of excess salt leads to the dissociation of the complex to give DNA with intact strands (21, 22). Prior to salt addition, a fraction of the enzyme-DNA complex is presumably in the form of the covalent intermediate that can be revealed by the addition of a protein denaturant. The addition of salt to the enzyme-DNA complex therefore appears to drive the dissociation of the enzyme and the rejoining of the DNA. In the absence of added Mg(II) and in the presence of excess EDTA, however, a significant fraction of the enzyme, termed the "salt-stable complex," was found to remain bound to single-stranded DNA upon addition of molar amounts of salt (21, 22).
The salt-induced reversal of DNA cleavage was exploited to test whether a mutant enzyme that showed DNA cleavage activity might be deficient in rejoining the broken DNA; following the DNA cleavage reaction by a mutant enzyme blocked in its DNA rejoining activity, the addition of salt would not be expected to rejoin the cleaved DNA. Lanes 1-3 of Fig. 4 depict the results of such a salt-reversal experiment with wild-type E. coli DNA topoisomerase I. The lane 1 sample in Fig. 4 was treated in the same way as that analyzed in lane 1 of Fig. 3. A DNA fragment 32P-labeled at a unique 5' end was denatured and incubated with the E. coli enzyme. SDS was then added to a final concentration of 1% to denature the enzyme and to reveal the formation of the covalent complex. For the sample run in lanes 2 and 3, incubation of the denatured DNA and the E. coli enzyme was carried out in the usual manner. Before the addition of SDS, however, NaCl was added to the lane 2 sample to a final concentration of 0.8 M, and NaCl and MgCl2 were added to the lane 3 sample, to 0.8 and 10 mM, respectively. The pattern shown in lane 3 was expected from results of the earlier studies; exposure of the enzyme-DNA complex to high salt would lead to rejoining of any cleaved DNA and dissociation of the enzyme from the DNA. Thus in contrast to the sample run in lane 1, which showed substantial amounts of cleaved DNA, the bulk of the DNA in the lane 3 sample appeared uncleaved. The high salt-induced rejoining of DNA was also observed in the absence of added Mg(II) (lane 2). When EDTA was added to 10 mM before the addition of NaCl, however, rejoining was largely abolished unless Mg(II) was added to a concentration of 1 mM or higher (data not shown) as observed previously (21, 22).
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DISCUSSION |
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We have applied alanine substitution mutagenesis in assessing the plausible roles of 12 residues, in addition to the nucleophile Tyr-319, in the catalysis of DNA breakage and rejoining by E. coli DNA topoisomerase I. Each of the residues was selected for site-directed mutagenesis based on the strict conservation of a polar group at that position in all homologues of phylogenetically diverse organisms including bacteria, eukarea, and archaea. It is therefore surprising that only substitution at Glu-9 or Arg-321 was found to affect transesterification between the active site tyrosine Tyr-319 and the scissile DNA phosphorous. Alanine substitution at the other 10 highly conserved positions, including Asp-111 and Glu-115, showed little effect on the DNA relaxation activity of the enzyme. In the crystal structure of the 67-kDa fragment of E. coli DNA topoisomerase I (15), Asp-111, Asp-113, and Glu-115 form an acidic triad that resembles the structure of a similar triad in E. coli DNA polymerase I, which binds one of the two Mg(II) ions that participate in exonucleolytic cleavage of DNA (26, 27). Asp-113 of E. coli DNA topoisomerase I was not selected for mutagenesis in the present work because of a lack of strict conservation at this position (9). The retention of enzymatic activity by alanine substitution at each of the other two positions of the acidic triad suggests, however, that this triad probably does not play a crucial role in the transesterification reactions catalyzed by the type IA family of enzymes; at least not all members of this triad are essential.
In a topoisomerase-catalyzed breakage of DNA, a proton is removed from the tyrosyl hydroxyl in the formation of the phosphotyrosine bond, and a proton is added to the deoxyribose 3' oxygen as it departs from the scissile phosphate. In the DNA rejoining reaction, the removal and addition of protons are reversed from the corresponding steps in the DNA breakage reaction. It has been suggested that a general base might be involved in proton removal and a general acid in proton addition in the transesterification reactions (4-6). There are two general issues in the breakage and rejoining of DNA by transesterification. First, is the DNA rejoining reaction, which normally follows DNA breakage and strand passage, the exact microscopic reversal of the DNA breakage reaction? If a chemical group is serving as a general acid in one reaction, does its conjugate necessarily act as a general base in the other? Second, could proton removal at one site and addition at another involve a multi-centered proton relay to coordinate proton transfer in one direction during the DNA breakage step and in the reverse direction in the DNA rejoining step?
Neither of the above two questions can be answered from the available experimental data. It has been predicted that there are large conformational changes in the enzyme-DNA complex during the various steps of DNA strand cleavage, passage, and rejoining (15), and therefore the DNA breakage reaction and the DNA rejoining reaction after strand passage might go through similar but nonidentical transition states. The lack of three-dimensional structural information for covalent and noncovalent enzyme-DNA complexes makes it a rather daunting task to arrive at a detailed mechanistic picture. At the present time, only one crystal structure, that of a 67-kDa fragment of the free enzyme, is known (15). Whereas in this crystal there is an extensive hydrogen-bonded network involving the active site tyrosine and other highly conserved polar residues, the positions of a significant number of these residues in the enzyme-DNA complexes are likely to be different from those seen in the protein crystal, and the possibility of a multi-centered proton relay cannot be assessed.
The mutagenesis approach itself has limitations in that it provides no information on the plausible involvement of backbone polar groups in the catalysis of DNA breakage and rejoining. Furthermore, it is also plausible that the strict sequence conservation criterion adopted by us in the selection of residues for mutagenesis might have overlooked essential side chains. Substitution of alanine for Arg-136, for example, was found to affect the DNA relaxation activity of the enzyme but not its DNA cleavage activity.1 Arg-136 was not selected in the mutagenesis work reported here because of uncertainties about its being strictly conserved. The ability of the mutant to cleave DNA suggests, however, that Arg-321 is not directly involved in the catalysis of DNA cleavage.
Despite the shortcomings, the results reported here provide several significant clues on the mechanism of type IA enzymes in general and E. coli DNA topoisomerase I in particular. First, no amino acid side chain appears to fit the role of a general base in proton removal from Tyr-319. Glu-9, being in domain I of the 67-kDa protein crystal structure, appears to be too far from the active site tyrosine Tyr-319, which is located in domain III. This is especially so in view of the prediction that the two domains I and III would move away from each other upon the binding of DNA (15). Although mutating Arg-321 to alanine reduces the DNA relaxation activity of the E. coli enzyme, the mutation has only a minor effect on DNA breakage (Figs. 3 and 4), and the mutant enzyme appears to be capable of rejoining the severed DNA strand (Fig. 4). The very high pKa of an arginyl side chain also makes it an unlikely general base at neutral pH. Because Arg-321 can be replaced by lysine without a significant reduction in DNA relaxation activity (Fig. 2), it appears to participate as a monodentate group. This positively charged side chain probably interacts with an oxygen of the scissile phosphate, or it might have a minor effect on Tyr-319 deprotonation through stabilization of the negatively charged phenolate intermediate (28-30).
Second, because of the much higher pKa value of a sugar hydroxyl relative to that of a tyrosyl hydroxyl, a well positioned proton acceptor is probably necessary to serve the role of a general base in proton removal from the 3' hydroxyl group during DNA rejoining and, similarly, that of a proton donor during DNA breakage. From the results reported here, Glu-9 appears to be the best candidate for these roles. Either by itself or through a bridging water, Glu-9 could serve a dual role of a proton donor and acceptor. Studies of the E9Q mutant protein suggest that such a dual role is apparently unaffected by the substitution of the glutamate at this position by glutamine.
The results shown in Fig. 4 raise questions on the role of Mg(II) in DNA cleavage and rejoining. It is known that Mg(II) is required in the removal of DNA negative supercoils by E. coli DNA topoisomerase I (7, 31) but not in the cleavage of DNA by the enzyme (2, 21). For the DNA rejoining reaction, a previous study showed that the transfer of an enzyme-linked DNA strand to the 3'-hydroxyl end of another DNA molecule requires Mg(II) (32), a finding that appears to contradict the results shown in Fig. 4, which suggest, superficially, that Mg(II) is not obligatory for intramolecular DNA rejoining.
This apparent conflict could be interpreted in two very different ways. In one view, Mg(II) is not required in the catalysis of either DNA breakage or rejoining by the E. coli enzyme. According to this view, in the supercoil removal reaction or the intermolecular rejoining reaction catalyzed by the enzyme, Mg(II) is mainly required for conformational changes in the enzyme-DNA complexes rather than for DNA strand cleavage and rejoining. The finding from fluorescence measurements that the binding of Mg(II) changes the environment of the tryptophan residues (33) is consistent with this interpretation. These conformation changes in the enzyme-DNA complexes are most likely of key importance in the E. coli DNA topoisomerase I-mediated movements of DNA strands but may be less crucial in DNA strand breakage and rejoining. A case in point is the finding with the E9Q mutant protein; it exhibits both DNA breakage and rejoining activity, yet it shows little DNA relaxation activity.
In the other view, there is an intrinsic asymmetry in Mg(II) requirement in DNA breakage and rejoining by E. coli DNA topoisomerase I; the divalent ion may be dispensable in the former, but it is required in the latter. In this view, the results in Fig. 4 could be attributed to either the presence of Mg(II) tightly associated with the enzyme or its inadvertent introduction when NaCl was added to 0.8 M in the salt-induced reversal experiment. Direct analysis of Mg(II) by atomic absorption spectroscopy showed no significant quantity of enzyme-bound Mg(II) in the preparation of enzyme used in the experiments reported in this work (datum not shown), which provides strong support of the notion that no Mg(II) is required in the DNA cleavage reaction (2, 21). In the salt-induced DNA rejoining reaction, the amount of Mg(II) from added NaCl is estimated to be less than 10 µM at a NaCl concentration of 0.8 M from analytical data provided by the supplier (Sigma ultra grade NaCl). This level of contamination is an order of magnitude lower than the free Mg(II) concentration required for a stoichiometric ratio of one bound Mg(II) per E. coli DNA topoisomerase I (33). Nevertheless, it is plausible that the enzyme-DNA complexes might have a much higher affinity for Mg(II) than the free enzyme. The finding that salt-induced rejoining of DNA cleaved by E. coli DNA topoisomerase I was largely absent in the presence of 10-20 mM EDTA, unless Mg(II) was added to 1 mM or higher, is consistent with the second interpretation. Whereas the available experimental data cannot rule out either interpretation, it seems clear that the concentration of Mg(II) required for removal of negative supercoils is much higher than that for DNA rejoining; thus one important role of Mg(II) is to facilitate conformational changes in the enzyme-DNA complexes.
Finally, it is noteworthy that in the study of DNA cleavage by the mutant Y319A, faint but discernible cleavage products were seen (Fig. 3, lane 4). These products could be attributed to the presence of a low amount of wild-type enzyme in the purified mutant protein, because the mutant protein used in the cleavage experiment was purified from strain BL21 topA+ cells overexpressing a plasmid-borne topA (Y319A). Such an explanation seems unlikely, however. For multiple preparations of the mutant proteins Y319A, E9A, and R321A from strain BL21 cells, only Y319A consistently showed low amounts of cleavage products (compare the patterns of lanes 2, 4, and 5 of Fig. 3). A more plausible interpretation is therefore that in the absence of a bulky tyrosyl group at position 319, a solvent water molecule could occupy this location and serve as a nucleophile in DNA cleavage.
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ACKNOWLEDGEMENTS |
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We thank Yuk-ching Tse-Dinh for exchanging results prior to publication, Qiyong Liu for help in the preparation of Fig. 1, Janie Ho for assistance in mutant construction, and Mark Ptashne for the use of an oligonucleotide synthesizer. We are most grateful to Robert Shapiro in the laboratory of Bert Vallee, who kindly measured for us the divalent metal ion contents in the preparation of E. coli DNA topoisomerase I used in this work.
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FOOTNOTES |
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* This work was supported by National Institutes of Health Grant GM24544 from the U. S. Public Health Service.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
To whom correspondences should be addressed: Dept. of Molecular
and Cellular Biology, Harvard University, 7 Divinity Ave., Cambridge,
MA 02138.
1 Y.-C. Tse-Dinh, personal communication.
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REFERENCES |
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