From the Department of Physiology, Trinity College, Dublin 2, Ireland
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ABSTRACT |
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Long term potentiation (LTP) in dentate gyrus is
impaired in aged rats, and this has been associated with an age-related
decrease in membrane arachidonic acid concentration. In this study, we considered whether the trigger for this age-related decrease in arachidonic acid might be increased lipid peroxidation stimulated by
the proinflammatory cytokine, interleukin-1. Groups of aged and
young rats were fed on a control diet or a diet supplemented with
-tocopherol and assessed for their ability to sustain LTP. Aged rats
fed on the control diet exhibited an impaired ability to sustain LTP
and analysis of tissue prepared from these rats exhibited increased
interleukin-1
, increased lipid peroxidation, and decreased membrane
arachidonic acid concentration compared with young rats fed on either
diet. Aged rats fed on the supplemented diet sustained LTP in a manner
indistinguishable from young rats, and the age-related increases in
interleukin-1
and lipid peroxidation and the decrease in membrane
arachidonic acid concentration were all reversed. We propose that
interleukin-1
may be the trigger that induces these age-related
changes and may therefore be responsible for the deficit in long term
potentiation in aged rats. The observation that
-tocopherol reverses
these changes is consistent with the hypothesis that some age-related
changes in hippocampus might derive from oxidative stress.
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INTRODUCTION |
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Aging is associated with compromised neuronal activity, perhaps typified by a deficit in cognitive function. One particular age-related impairment in neuronal function in the rat is a decrease in ability to sustain long term potentiation (LTP1; Refs. 1-5), which is a putative biological substrate for cognitive function and a model for learning and/or memory. Evidence suggests that a cascade of biochemical changes, initiated by activation of the N-methyl-D-aspartate subtype of glutamate receptor is required to support this form of synaptic plasticity in the dentate gyrus (6). Although the underlying cause of the age-related impairment in ability to sustain LTP is not known, down-regulation of several components of the biochemical cascade has been identified in aged rats. Thus, N-methyl-D-aspartate receptor activity is compromised with age (e.g. Ref. 7), whereas down-regulation of other factors that play a role in induction of LTP, like calcium handling by cells (1) and activation of enzymes such as phospholipase A2 (3) and protein kinases (8), has been reported. Similarly, factors that play a role in maintenance of LTP, like increased glutamate release, have also been shown to be compromised in hippocampus of aged rats (3, 5, 9). In addition, the more persistent aspects of LTP, which rely on increased synthesis of new proteins (10-12) and morphological changes (13), are also impaired in the hippocampus of aged rats (12, 14). Despite this immense body of data, the underlying cause of the impairment in ability of aged rats to sustain LTP is not known.
One unifying cause of the age-related changes in biochemical parameters might be a change in membrane composition. Thus, one might predict that, if membrane fluidity is altered, the activation of membrane proteins like receptors, ion channels, and membrane-associated enzymes might also be altered. Membrane arachidonic acid concentration has been shown to be markedly decreased with increasing age (3, 5); this is likely to decrease membrane fluidity (see Ref. 15) and is therefore one change that might explain significant changes in activity of the hippocampal membrane-associated proteins that impact on expression of LTP.
In the context of the change in membrane arachidonic acid concentration and the presumed increase in membrane rigidity, it is important to consider the growing body of evidence that supports the original hypothesis of Harman (16) that free radicals contribute to age-related changes in the central nervous system. One hallmark of free radical damage is lipid peroxidation; most reports indicate that lipid peroxidation increases with increasing age (17, 18), and this is supported by the observation that lipofuscin, one characteristic reaction product, accumulates in the aged brain (18). With specific reference to the hippocampus, recent evidence from this laboratory has indicated that there is an age-related increase in lipid peroxidation in the hippocampus of aged rats (19), supporting earlier findings (Ref. 20; see also Ref. 21). It has been acknowledged for many years that lipid peroxidation will profoundly affect membrane composition since it results in depletion of polyunsaturated fatty acids (22); therefore, the increase in lipid peroxidation in hippocampus may be the underlying cause of the age-related decrease in membrane arachidonic acid concentration (3, 5).
An increase in lipid peroxidation will be triggered by an accumulation
of reactive oxygen species, and this arises from a compromise in
antioxidant defenses. Neuronal tissue is particularly vulnerable to
oxidative insults; the vulnerability results from high oxygen
consumption of brain tissue, coupled with modest antioxidant defense
(23), high concentrations of iron, particularly in hippocampus (24),
and high concentrations of polyunsaturated fatty acids, which are
target substrates for reactive oxygen species (25). Accumulation of
oxidative damage is readily observed in neuronal tissue (26, 27), and
there is substantial evidence that expression of the proinflammatory
cytokine interleukin-1 (IL-1
) and its receptor are increased in
brain tissue exhibiting oxidative damage (28).
The importance of -tocopherol, one naturally occurring antioxidant,
for normal neuronal cell function has been defined most clearly by
studies that assess the consequences of vitamin E deficiency. Thus,
vitamin E deficiency in patients leads to, among other problems, neurological deficiencies (29), whereas prolonged vitamin E deficiency
in rats leads to demyelination of axons and gliosis (30). Maintenance
of rats on a vitamin E-deficient diet markedly reduced the vitamin
concentration in whole brain (31) or cortex (32), and this was
associated with increased lipid peroxidation and increased
susceptibility to oxidative stress (17, 31, 32), whereas protection
against oxidative damage was offered by vitamin E administration (31).
In parallel, vitamin E-deficient rats had impaired ability to sustain
LTP (33), whereas
-tocopherol induced synaptic potentiation
(34).
In light of these observations, we considered that the impaired ability
of aged rats to sustain LTP might be due to a decreased in membrane
arachidonic acid. Our hypothesis is that this change is triggered by
increased lipid peroxidation, which in turn is triggered by IL-1,
perhaps through formation of reactive oxygen species. To test this
hypothesis, aged and young rats were fed on either a control diet or a
diet enriched in
-tocopherol and L-ascorbate (to limit
oxidation of
-tocopherol). At the end of this period, rats were
analyzed for their ability to sustain LTP in dentate gyrus in
vivo and these data were interpreted in parallel with analyses
performed on tissue prepared from hippocampus obtained from these rats
at the end of the electrophysiological recording period. We report that
dietary supplementation with antioxidant vitamins reversed the
age-related decrease in
-tocopherol concentration and concomitantly
restored ability of these rats to sustain LTP. The data presented are
consistent with the view that an age-related increase in endogenous
concentrations of IL-1
may be the underlying cause of certain
neuronal impairments in hippocampus of aged rats.
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EXPERIMENTAL PROCEDURES |
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Animals--
Groups of male Wistar rats (average ages: 4 months
and 22 months) were used in these experiments. Animals were housed in
groups of 2-4 under a 12-h light schedule. Ambient temperature was
controlled between 22 and 23 °C. Food and water was freely
available, and intake was measured daily for 2 weeks before
commencement of the experiment and throughout the experiment. Aged (22 months) and young (4 months) rats were then randomly subdivided into
two groups. One group received normal laboratory chow with added
DL--tocopheryl acetate (250 mg/rat/day dissolved in corn
oil; Beeline Healthcare, Dublin, Ireland) and drinking water to which
L-ascorbic acid (250 mg/rat/day; Beeline Healthcare) was
added. L-Ascorbic acid was included since it has the
ability to recycle vitamin E by reducing the oxidized form (35, 36).
The second group received normal laboratory chow with corn oil added to
ensure isocaloric intake with the first group. Food and water intake
did not vary between groups, and there was no significant difference in
daily food and water intake before and after dietary modifications were
made. Rats were fed on the respective control or supplemented diet for 3 months; at the end of this time, ability of animals to sustain LTP
was assessed. Animals were housed in the BioResources Unit at Trinity
College, Dublin under veterinary supervision for the duration of this
experiment.
Induction of LTP in Vivo-- Animals were anesthetized by intraperitoneal injection of urethane; the absence of a pedal reflex was used as the indicator of adequate anesthesia. Young animals initially received 1.5 g/kg urethane, and an additional increment of up to 0.5 g/kg if required; the mean dose of urethane administered to 4-month-old rats was 2.07 g/kg (± 0.11; S.E.). In the case of 22-month-old animals, the initial dose of urethane was reduced to 1.0 g/kg and topped up if necessary; the mean dose of urethane given to 22-month-old rats was 1.63 g/kg (± 0.10).
LTP was induced in perforant path-granule cell synapses as described previously (5). Briefly, rats were placed in a head holder in a stereotaxic frame and a window of skull was removed to allow placement of a bipolar stimulating electrode in the perforant path (4.4 mm lateral to lambda) and a unipolar recording electrode in the dorsal cell body region of the dentate gyrus (2.5 mm lateral and 3.9 mm posterior to bregma). The depths of the stimulating and recording electrodes were adjusted to maximize the slope of the excitatory postsynaptic potential (EPSP) and the stimulating intensity was adjusted to produce a population spike amplitude of about 1 mV. Test shocks were delivered unilaterally at 30-s intervals and after a 10-min control period, three trains of stimuli (250 Hz for 200 ms) were delivered with an intertrain interval of 30 s. (Previous experiments indicated that this stimulation paradigm produced saturation of LTP in perforant path-granule cell synapses.) Recording at test shock frequency then resumed for 40 min. Data were stored on disk for later analysis. At the end of the recording period, rats were killed by cervical dislocation. The dentate gyri from the ipsilateral (i.e. tetanized) and contralateral (i.e. untetanized) sides were cross-chopped (350 µm × 350 µm) and frozen separately in 1 ml of Krebs solution (composition of Krebs in mM: NaCl 136, KCl 2.54, KH2PO4 1.18, MgSO4·7H2O 1.18, NaHCO3 16, glucose 10, CaCl2 1.13) containing 10% dimethyl sulfoxide as described previously (37). The remaining hippocampal (i.e. whole hippocampus minus dentate gyrus) tissue was treated in the same way, and all samples were stored atAnalysis of IL-1 Expression--
We used an enzyme-linked
immunosorbent assay for analysis of IL-1
(DuoSet ELISA Development
System, Genzyme Diagnostics). Slices of dentate gyrus prepared from
aged and young animals were homogenized in Tris buffer (50 mM,; 250 µl, pH 7.4) containing 1 mM EDTA, 5 mM dithiothreitol, 0.1 mg/ml trypsin inhibitor, 50 µM leupeptin, and 50 µM aprotonin. For
analysis of IL-1
, 96-well plates were coated with 100 µl of
capture antibody (2.0 µg/ml final concentration, diluted in 0.1 M sodium carbonate buffer, pH 9.5; monoclonal hamster
anti-mouse IL-1
antibody) and incubated overnight at 4 °C. Wells
were washed several times with phosphate-buffered saline (PBS)
containing 0.05% Tween 20 and then blocked for 2 h at 37 °C
with 250 µl of blocking buffer (PBS, pH 7.3; 0.1 M with
4% bovine serum albumin). Blocking buffer was aspirated, and aliquots
(100 µl) of samples or IL-1
standards (0-1000 pg/ml) were added
to each well and incubated for 1 h at 37 °C. Plates were
washed, secondary antibody (100 µl; final concentration 0.8 µg/ml
in PBS containing 0.05% Tween 20 and 1% bovine serum albumin; biotinylated polyclonal rabbit anti-mouse IL-1
antibody) was added
to each well, and incubation continued for 1 h at 37 °C. The
plates were then washed, 100 µl of detection agent (horseradish peroxidase-conjugated streptavidin; 1:1000 dilution in PBS containing 0.05% Tween 20 and 1% bovine serum albumin) was added to each well,
incubation continued for another 15 min at 37 °C, and plates were
again washed. Aliquots of substrate (100 µl; tetramethylbenzidine liquid substrate; Sigma) were added, and the plate was incubated at
room temperature for 10 min. Absorbance was read at 450 nm within 30 min. Protein concentrations were determined (38), and results were
expressed as nanograms of IL-1
/mg of tissue corrected for protein
concentration.
Analysis of Lipid Peroxidation--
Formation of malondialdehyde
(MDA) was used to assess lipid peroxidation as described previously
(39). Aliquots (10 µl) of homogenate prepared from dentate gyrus were
incubated at 37 °C for 60 min, at which time 8.1% (w/v) SDS (30 µl), 20% acetic acid (pH 3.5 with NaOH; 225 µl), and 0.8% (w/v)
thiobarbituric acid (225 µl) were added. The volume was then adjusted
with H2O to 600 µl, and the samples were incubated for
another 60 min at 95 °C, cooled at room temperature, and absorbance
assessed at 532 nm with reference to a standard curve made using
1,1,3,3-tetramethoxypropane. In some experiments, IL-1 (3.5 ng/ml)
or hydrogen peroxide (5 mM) were added to analyze its
effect on lipid peroxidation; the effect of antioxidants
(±)-
-tocopherol phosphate (disodium salt; 200 µM) or
melatonin (5 mM) on IL-1
- or hydrogen peroxide-induced effects were also assessed. Results were expressed as nanomoles of
MDA/mg of protein for the in vitro experiments. Since we
observed that values for protein/mg of tissue were different in
hippocampal preparations obtained from aged and young rats, data from
these experiments were expressed as nanomoles/mg of tissue corrected for protein concentration.
Analysis of Reactive Oxygen Species Formation--
The formation
of reactive oxygen species was assessed in hippocampal tissue (minus
dentate gyrus) as described previously (40). The method relies on
oxidation of the non-fluorescent probe, 2',7'-dichlorofluorescein
diacetate, by reactive oxygen species, to the highly fluorescent
2',7-dichlorofluorescein. To assess reactive oxygen species production,
either homogenate or the synaptosomal pellet, P2, was
resuspended in 1 ml ice-cold 40 mM Tris buffer (pH 7.4).
Aliquots (1 ml) of synaptosomes or homogenate were incubated with
2',7'-dichlorofluorescein diacetate (10 µl; final concentration 5 µM; from a stock solution of 500 µM in
methanol) at 37 °C for 15 min. Analysis was performed in hippocampal
preparations under control conditions (i.e. no additions), in the presence of IL-1 (3.5 ng/ml) or in the presence of IL-1
(3.5 ng/ml) and (±)-
-tocopherol phosphate (disodium salt; 200 µM). In a separate group of experiments, we analyzed
reactive oxygen species production in tissue prepared from aged and
young rats that were fed on the control diet, and aged and young rats that were fed on the experimental diet. To terminate the reaction, the
dye-loaded synaptosomes were centrifuged at 13,000 × g
for 8 min. The pellet was resuspended in 3 ml of ice-cold 40 mM Tris buffer, pH 7.4. Aliquots of resuspended
synaptosomes (750 µl) were incubated in the presence or absence of
IL-1
(3.5 ng/ml). Fluorescence was monitored at a constant
temperature of 37 °C immediately before stimulation with IL-1
,
and 15 min after stimulation, at 488 nm excitation (bandwidth 5 nm) and
525 nm emission (bandwidth 20 nm). Reactive oxygen species formation
was quantitated from a standard curve of 2',7-dichlorofluorescein in
methanol (range: 0.05-1 µM). Results were expressed as
nanomoles/mg of tissue corrected for protein.
Analysis of Arachidonic Acid Concentration-- Aliquots (150 µl) of homogenate prepared from dentate gyrus were added to chloroform:methanol (2:1 (v/v); 1 ml) and fatty acids were extracted by vigorous shaking for 10 min followed by centrifugation at 1000 × g for 5 min to separate the phases. The aqueous layer was discarded, and the chloroform phase was evaporated under nitrogen and resuspended in ethanol for analysis. Arachidonic acid was analyzed as its 2-nitrophenylhydrazine derivatives by reverse phase HPLC (41). Fatty acids were derivatized by adding 2-nitrophenylhydrazine-HCl solution (0.02 M 2-nitrophenylhydrazine-HCl in 40 mM HCl-ethanol (3:1, v/v)) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride solution (0.25 M 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride in ethanol mixed in equal volumes with 3% ethanolic pyridine), and incubated at 60 °C for 20 min. After addition of KOH (15% (w/v) in MeOH:H2O, 80:20) samples were incubated at 60 °C for 15 min and cooled in running water. Fatty acid derivatives were concentrated; n-hexane and phosphate buffer (0.033 M, pH 6.4, in 0.5 M HCl) were added, samples were vortex mixed for 30 s, centrifuged for 5 min at 1500 × g, and the hexane phase evaporated to dryness under nitrogen. For HPLC analysis, samples were resuspended in methanol and injected onto a Microsorb C18 column (maintained in a column oven at 30 °C), and fatty acid derivatives were separated in isocratic mode with a mobile phase of 85% methanol:15% water (maintained at pH 4.5 with HCl) and detected by UV spectroscopy at 230 nm. Arachidonic acid concentration, expressed as micromoles/mg of tissue, was estimated using the external standard method.
Analysis of -Tocopherol--
Hippocampal preparations were
homogenized in ice-cold Krebs solution. Aliquots (150 µl) were
incubated in the presence of ethanol containing 0.025%
butyl-hydroxytoluene (150 µl), 25% ascorbic acid (70 µl), 10%
potassium hydroxide (135 µl) for 30 min at 60 °C. Hexane (540 µl) containing 0.025% butyl-hydroxytoluene was added, and samples
were vortex-mixed for 1 min and centrifuged at 1500 rpm for 6 min. The
hexane phase was removed and evapourated to dryness under nitrogen. For
HPLC analysis, dried samples were resuspended in methanol (150 µl)
containing 0.025% butyl-hydroxytoluene and 30-µl volumes were
injected onto an Intersil C18 column (maintained at 40 °C).
Separation of
-tocopherol was achieved using a mobile phase of 75%
acetonitrile:25% methanol at a flow rate of 1.2 ml/min and samples
were detected by uv spectroscopy at 292 nm.
-Tocopherol concentration was estimated by the external standard method and expressed as micrograms/mg of tissue, corrected for protein
concentration.
Statistical Analysis-- Either Student's t test or a one-way analysis of variance (ANOVA) was performed to determine whether there were significant differences between conditions. When ANOVA was used and when this analysis indicated significance (at the 0.05 level), post hoc Student-Neumann-Keul test analysis was used to determine which conditions were significantly different from each other.
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RESULTS |
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The mean pretetanus EPSP slope was similar in 4-month-old and
22-month-old rats (1.13 mV/ms ± 0.11 (S.E.) and 1.11 mV/ms ± 0.09, respectively). Dietary supplementation with -tocopherol and
ascorbate significantly increased mean EPSP slope to 1.40 mV/ms (± 0.10 S.E.; p < 0.05; Student's t test for
independent means) in young rats but not aged rats (mean value, 1.03 mV/ms ± 0.06). There was an age-related decrease in mean
amplitude of the response; the mean values were 3.51 ± 0.35 mV
and 2.18 ± 0.17 mV in 4-month-old and 22-month-old rats fed on
the control diet (p < 0.01; Student's t
test for independent means). This was partially reversed by dietary
supplementation; the corresponding mean values were 3.10 ± 0.21 mV and 2.59 ± 0.18 mV. The stimulus strength required to induce a
response at which the spike became evident was slightly, although not
significantly, greater in aged (4.29 ± 0.54 V) compared with
young (3.57 ± 0.21 V) rats.
Dietary supplementation with vitamins had little effect on induction of LTP in either 4-month-old or 22-month-old rats. In contrast, a significant effect on maintenance of LTP, particularly in aged rats, was observed; in this study, we compared mean EPSP slopes and mean amplitude of the responses in the last 5 min of the experiment with the values in 5 min immediately before tetanic stimulation. In 4-month-old rats, the mean percentage changes in EPSP slopes (± S.E.) were 134.27% (2.67) in rats fed on control diet compared with 126.88% (1.83) in rats fed on the experimental diet; these values were not significantly different (Fig. 1A). The corresponding values for mean amplitude of the response were 142.76% (3.56) and 128.55% (1.70) in the groups fed on control and experimental diets, respectively; thus mean amplitude of the response was significantly decreased in rats fed on the experimental diet (p < 0.05; Student's t test for independent means; data not shown).
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Fig. 1B indicates that LTP, as measured by the change in EPSP slope, was impaired in aged rats fed on the control diet compared with young rats, but that this impairment was reversed in aged rats fed on the experimental diet. The mean percentage changes (± S.E.) in EPSP slope in the last 5 min of the experiment compared with the 5 min immediately before tetanic stimulation were 107.99% (2.92) in aged rats fed on control diet compared with 124.07% (1.89) in aged rats fed on the experimental diet. The corresponding values for the mean percentage change in the amplitude of the response were 109.01% (2.47) and 131.51% (2.56) for aged rats fed on the control and experimental diets, respectively (data not shown).
We analyzed -tocopherol concentration in hippocampal homogenate
prepared from young and aged rats in both dietary groups; data are
presented for all samples analyzed, i.e. 11 young rats and
12 aged rats. Analysis revealed that there was an age-related decrease
in
-tocopherol (p < 0.05; ANOVA; Fig.
2A). Our results indicate
that, although dietary supplementation with
-tocopherol and
ascorbate did not significantly affect mean
-tocopherol
concentration in hippocampus of young rats, it significantly increased
it in aged rats and completely reversed the age-related decrease.
Analysis of the correlation between
-tocopherol concentration and
ability of rats to sustain LTP in dentate gyrus indicated a marked
age-related difference. We found that, although there was no
significant correlation between these parameters in young rats (Fig.
2B; r = 0.013; p = 0.97), a
significant correlation was observed in aged rats (Fig. 2C;
r = 0.65; p = 0.022).
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Fig. 3C indicates that there was no significant effect of dietary manipulation on membrane arachidonic acid concentration in dentate gyrus. However, mean arachidonic acid concentration was significantly decreased in tissue prepared from aged rats fed on the control diet, compared with young rats fed on either diet (p < 0.05; ANOVA). The age-related decrease in arachidonic acid concentration was reversed in 22-month-old rats fed on the experimental diet, and the mean value was significantly higher in aged rats fed on the experimental diet compared with that in aged rats fed on the experimental diet (p < 0.05; ANOVA).
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We analyzed lipid peroxidation in aliquots of the same tissue used for
analysis of arachidonic acid, since an increase in lipid peroxidation
might account for the decreased membrane arachidonic acid
concentration. Fig. 3B indicates that there was an
age-related increase in lipid peroxidation in rats fed on the control
diet (p < 0.01; ANOVA). Although dietary manipulation
had no significant effect on lipid peroxidation in 4-month-old rats,
dietary supplementation with -tocopherol and ascorbate reversed the
age-related increase in this measure. The mean value was significantly
lower in aged rats fed on the experimental diet compared with that in
aged rats fed on the experimental diet (p < 0.01;
ANOVA).
Previous evidence indicated that one trigger for lipid peroxidation was
IL-1; therefore, we analyzed the concentration of this cytokine in
aliquots of the same tissue used in the analyses described above. Fig.
3A indicates that IL-1
concentration was significantly
increased in aged rats fed on the control diet compared with young rats
fed on either diet (p < 0.01; ANOVA). Dietary supplementation with
-tocopherol and ascorbate reversed the
age-related increase in IL-1
concentration in dentate gyrus; the
mean value in aged rats fed on the experimental diet was similar to
those observed in young rats and significantly greater than that in the
group of aged rats fed on the experimental diet (p < 0.01; ANOVA).
In parallel with the coupled age-related increases in IL-1 and lipid
peroxidation in vivo, our data revealed that IL-1
significantly increases lipid peroxidation in hippocampal homogenate
prepared from 4-month-old rats in vitro (p < 0.01; ANOVA; Fig. 4A). The data indicate that this effect was mimicked by hydrogen peroxide (p < 0.01; ANOVA), and both the IL-1
-induced
increase and the hydrogen peroxide-induced increases were inhibited by
-tocopherol and the antioxidant, melatonin (Fig. 4A).
These results suggested that IL-1
induced its effect by formation of
reactive oxygen species. This supported by the demonstration that
IL-1
induced a significant increase in reactive oxygen species
production in hippocampus prepared from 4-month-old rats in
vitro (p < 0.05; ANOVA; Fig. 4B); we
observed that this effect was inhibited by
-tocopherol. Consistent
with the in vitro findings, we report that there was a
significant age-related increase in reactive oxygen species production
in hippocampus prepared from rats fed on the control (Fig.
4C; p < 0.05; ANOVA), but this effect was reversed in tissue prepared from aged rats that were fed on the experimental diet; thus, mean reactive species production was significantly lower in 22-month-old rats fed on the diet supplemented with
-tocopherol and ascorbate, compared with that in 22-month-old rats fed on the control diet (p < 0.05; ANOVA).
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DISCUSSION |
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We addressed the hypothesis that the impaired ability of aged rats
to sustain LTP might be due to decreased membrane arachidonic acid
concentration triggered by oxidative stress. If this hypothesis is to
be accepted, it must be predicted that reversing the cause of the
oxidative stress will restore ability of aged rats to sustain LTP. The
data presented indicate that dietary supplementation with the
antioxidants -tocopherol and ascorbate reversed the age-related
decreases in
-tocopherol and arachidonic acid concentrations in
dentate gyrus and also reversed the age-related impairment in LTP.
Aged rats exhibited an impaired ability to sustain LTP in perforant
path-granule cell synapses, confirming previous findings (1-5).
Several age-related changes have been proposed as factors that might
lead to this impairment in LTP (7-14); however, because many of the
age-related changes involve membrane-associated functions, we have
proposed that altered membrane composition, specifically a decrease in
membrane arachidonic acid concentration might be the underlying cause
(3, 5). In support of this hypothesis, we have demonstrated that, if
membrane arachidonic acid concentration in hippocampus of aged rats is
restored to levels observed in young rats reversed by dietary
supplementation with arachidonic acid and its precursor -linolenic
acid, the impairment in LTP is reversed (5).
A number of reports have indicated that aging is associated with changes in membrane composition (15, 42, 43), and, although the underlying cause remains unclear, it has been accepted that lipid peroxidation, apparently triggered by reactive oxygen species, can lead to a decrease in membrane polyunsaturated fatty acids (23), which contributes to membrane rigidity (22, 44). We have found that lipid peroxidation is increased, whereas membrane arachidonic acid concentration is decreased, in hippocampal tissue prepared from aged rats2; these data, albeit obtained in separate experiments, suggested that arachidonic acid may be one target molecule for lipid peroxidation. The results of the present study develop this argument further, inasmuch as, in separate aliquots prepared from the same sample of dentate gyrus, we observed an age-related increase in lipid peroxidation coupled with an age-related decrease in membrane arachidonic acid concentration. Although this cannot be considered as conclusive proof of a causal relationship between these measures, it is consistent with the hypothesis that lipid peroxidation depletes membrane arachidonic acid. We also report here that there was an age-related increase in reactive oxygen species production in hippocampus, supporting the view that formation of free radicals contributes to changes associated with aging (45).
In theory, increased formation of reactive oxygen species will occur if
there is down-regulation of the enzymatic defense strategies or a
decrease in concentration of reactive oxygen species scavengers, like
vitamins E or C or glutathione. Results from studies investigating
antioxidant enzyme activities in aged brain are generally consistent
with reduced ability to cope with free radicals (46-48), although
there is no consensus about age-related changes in -tocopherol
concentration in brain, with some reports indicating an increase (27)
and others reporting no change (49) depending on the species, brain
area, and precise age. The results of the present experiment indicate a
marked age-related decrease in
-tocopherol concentration in dentate
gyrus. This compromise in one component of the antioxidant defense
strategy might be sufficient to account for the increase in free
radical formation described in the present study in the hippocampus and
previously in other brain areas (26).
Our prediction was that, if the age-related increases in reactive
oxygen species production and lipid peroxidation could be inhibited,
then the age-related decrease in membrane arachidonic acid
concentration and impairment in LTP would be reversed. The data
presented demonstrate that aged rats which received a dietary supplement of -tocopherol and ascorbate for 12 weeks sustained LTP
in a manner indistinguishable from young rats. The age-related decrease
in
-tocopherol concentration in dentate gyrus was also reversed,
consistent with the previous observation that incorporation of the
vitamin occurs after a relatively long period of dietary supplementation (29). We observed that there was an increase in EPSP
slope in young rats, but not aged rats, which received the experimental
diet. One explanation for this finding might be that the action of
-tocopherol relies on increased release of arachidonic acid (50),
which is attenuated in aged rats due to decreased availability.
The argument that the underlying cause of the age-related impairment in
LTP was due to decreased -tocopherol concentration is supported by
the observation that there is a significant correlation between
-tocopherol concentration in hippocampus and ability of aged rats to
sustain LTP. Interestingly, no correlation was observed in young rats.
A significant role for vitamin E in generation of LTP in area CA1
in vitro has been suggested by previous studies. Thus rats
fed on a vitamin E-deficient diet showed impaired ability to sustain
LTP (33), whereas application of
-tocopherol induced a slowly
developing form of potentiation (34).
-Tocopherol might have a direct effect on LTP, although it is more
likely that its effect may be explained by its antioxidant action (22).
This is supported by the observation that the impairment in LTP in
vitamin E-deficient rats (33) is mimicked by application of free
radicals (51). The data presented here provide strong evidence to
further support this hypothesis; we observed that age was associated
with a concomitant decrease in
-tocopherol and increases in reactive
oxygen species and increase lipid peroxidation, all of which were
reversed by dietary manipulation with antioxidant. The tight coupling
of these parameters, in tandem with age-related and diet-related
changes in LTP, are suggestive of a causal relationship between these
parameters.
Lipid peroxidation was enhanced in dentate gyrus of aged compared with young rats; this observation confirms our previous data in whole hippocampus (19) and the previous data of others (Refs. 17, 18, and 20; but see Ref. 21). The parallel decrease in membrane arachidonic acid concentration 1) supports the finding that there is an increase in lipid peroxidation and 2) suggests that this polyunsaturated fatty acid might be one target lipid molecule for oxidation.
Previous observations have indicated changes in membrane composition,
particularly in arachidonic acid concentration, with age (3, 19, 42,
43). Arachidonic acid modulates synaptic function (3, 5), and its
membrane concentration correlates with ability of aged rats to sustain
LTP (3); therefore, it was predicted that the age-related changes in
arachidonic acid may underlie some of the impairments in neuronal
function that accompany aging (e.g. Ref. 15). Significant
support for this hypothesis was obtained when we established that
reversing the age-related decrease in membrane arachidonic acid
concentration reversed the age-related impairment in LTP (5). This is
further supported by the results of the present study, which
demonstrated that dietary supplementation with -tocopherol and
ascorbate reversed the age-related decrease in membrane arachidonic
acid concentration and restored ability of aged rats to sustain LTP. We
propose that this effect is secondary to its effect on reactive oxygen
species production and lipid peroxidation.
We report that there was an age-related increase in IL-1
concentration in dentate gyrus. Although it must be considered that the
procedures involved in tissue preparation contributed to the increase
in IL-1
concentration and that this was exacerbated in tissue in
which
-tocopherol concentration was decreased, the data presented
support our previous observation in whole hippocampus (52, 53). The
data also indicate a correlation between IL-1
concentration in
hippocampus and ability to sustain LTP; thus, IL-1
was increased in
hippocampus of aged rats maintained on the control diet (compared with
young rats), and these rats showed impaired ability to sustain LTP,
whereas the experimental diet reversed both the age-related increase in
IL-1
and the age-related deficit in LTP. Thus, a causal relationship
between the two parameters may exist, consistent with the findings that
IL-1
inhibited full expression of LTP in perforant path-granule cell
synapses in vitro (54) and in vivo (53). The
mechanism by which IL-1
inhibits LTP might relate to the finding
that IL-1
inhibits glutamate release and calcium influx in the
hippocampus in vitro (52), but the molecular mechanism
underlying its inhibitory effect remains to be established. It has been
reported that IL-1
increased reactive oxygen species production in
pancreatic islets (55), and we report here that IL-1
also increased
reactive oxygen species production in hippocampus in vitro.
This action of IL-1
might explain its inhibitory effect on LTP (53,
54), whereas the age-related increase in endogenous IL-1
together
with the compromised antioxidant defenses in the aged brain might
explain the age-related compromise in LTP (1-3), since reactive oxygen
species inhibit LTP (51). This action might also couple two parallel
changes reported here, i.e. the age-related increase in
endogenous IL-1
and the impaired ability of aged rats to sustain
LTP. Thus, IL-1
may trigger formation of reactive oxygen species
in vivo, leading to increased lipid peroxidation and, in
turn, to a decrease in membrane arachidonic acid concentration. The
observation that the age-related increase in IL-1
concentration was
reversed by the experimental diet, in parallel with the changes in the
other parameters, supports this proposal; therefore, the data presented are broadly consistent with the "free radical hypothesis" of aging. The mechanism by which dietary supplementation with
-tocopherol and
ascorbate reverses the age-related increase in IL-1
concentration is
unclear, but may be due to the antioxidant property of these vitamins
since it has been reported that stress, including oxidative stress,
induces IL-1
(28).
Current and previous (3, 5) data have indicated that one factor which
contributes to the poor ability of aged rats to sustain LTP is a
decrease in membrane arachidonic acid; the present data indicate that a
decrease in -tocopherol concentration in dentate gyrus also
correlates with the deficit in LTP. When these decreases are reversed,
as they were by dietary supplementation with
-tocopherol and
ascorbate, ability of aged rats to sustain LTP is restored; these
observations are consistent with the view that both rely on the ability
of the hippocampus to sustain an adequate antioxidant defenses in age.
We propose that the age-related increase in endogenous IL-1
in
hippocampus may be the trigger leading to the biochemical deficits
observed, but additional studies are clearly necessary to provide
conclusive evidence to support this proposal.
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ACKNOWLEDGEMENT |
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We are grateful to Beeline Healthcare, Dublin,
Ireland for providing us with -tocopherol and ascorbate.
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FOOTNOTES |
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* This work was supported by Forbairt (Ireland) and the Provost's Academic Development Fund (Trinity College, Dublin, Ireland).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 353-1-608-1770;
Fax: 353-1-679-3545; E-mail: lynchma{at}tcd.ie.
1
The abbreviations used are: LTP, long term
potentiation; ANOVA, analysis of variance; IL-1, interleukin-1
;
PBS, phosphate-buffered saline; MDA, malondialdehyde; HPLC, high
performance liquid chromatography; EPSP, excitatory postsynaptic
potential.
2 C. A. Murray and M. A. Lynch, unpublished data.
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REFERENCES |
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