From the Departments of Cancer Biology and § Biochemistry, Bowman Gray School of Medicine and Comprehensive Cancer Center of Wake Forest University, Winston-Salem, North Carolina 27157
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ABSTRACT |
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We have previously reported that the adenovirus E1A oncogene represses the transcription of the H subunit of the mouse ferritin gene. Subsequent analyses defined FER-1, a 37-nucleotide sequence located 4.1 kilobases proximal to the start site of transcription, as the target of E1A-mediated transcriptional repression and as an enhancer of the ferritin H gene. FER-1 is composed of an AP1-like sequence followed by an element with dyad symmetry. To achieve maximal enhancer activity and transcriptional repression by E1A, both elements were essential. Using gel retardation assays, we now demonstrate that the binding complex for the AP1-like sequence of FER-1 contains JunD, FosB, and ATF1. Furthermore, JunD and FosB were able to activate FER-1 enhancer activity by transient cotransfection with ferritin H-chloramphenicol acetyltransferase reporter constructs. This augmented enhancer activity was inhibited by E1A. In addition, we have defined the minimal sequence in the dyad element of FER-1 required for protein interaction. This was determined to be a C-rich sequence to which Sp1 and Sp3 bind. Experiments with recombinant proteins indicate that members of both transcription factor families simultaneously bind FER-1. Taken together, these results elucidate molecular mechanisms involved in the transcriptional regulation of a pivotal gene in iron metabolism and provide insights into the contribution of the Sp1 family to the activation of AP1-dependent enhancers.
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INTRODUCTION |
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Ferritin is a ubiquitous and highly conserved iron binding protein
that plays a role in the storage and partitioning of iron for
intracellular use. It consists of two types of subunits, designated H
and L. They assemble to form a 24-subunit ferritin shell in which the
H:L subunit ratio reflects the tissue type and physiological status of
the cell (reviewed in Ref. 1). The H and L subunits of ferritin are
encoded by independent genes (2), and expression of these genes is
regulated at both transcriptional and posttranscriptional levels. The
level of intracellular iron is the principal posttranscriptional regulator of ferritin. Translation of both H and L subunits of ferritin
are coordinately regulated through the interaction of RNA binding
proteins with the iron-responsive element in the 5-untranslated region
of the ferritin H and L mRNAs (see Ref. 3 for review). Ferritin
synthesis is also regulated at transcriptional level, in which the
preferential modulation of the H subunit rather than the L subunit of
ferritin is frequently observed. For instance, we reported that the
transcription of the H subunit of ferritin is selectively activated by
the inflammatory cytokines tumor necrosis factor (4-6) or interleukin
1 (4, 7).
In addition to these cytokines, we found that the adenovirus E1A
oncogene preferentially represses the transcription of the H subunit of
the ferritin gene in NIH3T3 mouse fibroblasts (8). To our knowledge,
E1A is the only negative modulator of transcription of ferritin H gene
identified to date, and this was another example showing independent
regulation of transcription in the H and L subunits of ferritin gene.
We have recently identified the target sequence (termed FER-1)
responsible for E1A-mediated transcriptional repression of the mouse
ferritin H gene (9). FER-1 is a
37-bp1 composite element
comprising an AP1-like sequence followed by an element with dyad
symmetry, and it is located approximately 4.1 kb 5 to the
transcription initiation site of the mouse ferritin H gene. Our
previous studies demonstrated that FER-1 serves as an enhancer of the
mouse ferritin H gene, which augments the transcription of this gene in
a position- and orientation-independent manner. We also found that
within FER-1, the AP1-like sequence alone had moderate enhancer
activity, the element with dyad symmetry by itself had no enhancer
activity, and both elements were required for maximal enhancer activity
(9). Thus, the two elements of FER-1 cooperatively activate the
transcription of the ferritin H gene. In addition, E1A decreased the
binding of Jun and Fos family members to the AP1-like element of FER-1
in the absence of an alteration in the binding of nuclear factors to
the dyad symmetry sequence of FER-1 (9), suggesting that the main
target for transcriptional repression of the ferritin H gene by E1A is the family of AP1 transcription factors in the FER-1 binding
complex.
AP1 is a sequence-specific transcription factor consisting of a dimer of Jun and Fos family members (Jun family: c-Jun, JunB, and JunD; Fos family: c-Fos, FosB, fra1, and fra2). Members of the Jun family can form homo- and heterodimers and recognize a consensus DNA sequence (TGAGTCA) or related sequences. Fos family members cannot dimerize with each other but can associate with any member of the Jun family and augment transcriptional activation when compared with dimers of Jun family members (reviewed in Ref. 10). In addition to Jun and Fos family members, some ATF/CREB family members can recognize the AP1 binding sequence by dimer formation with Jun or Fos family members (11). Thus, the AP1 or AP1-related nucleotide sequences can bind at least three large families of transcription factors (Jun, Fos, and ATF/CREB families), and specific dimer formation induced by different environmental stimuli may determine the specificity of genes activated through AP1 binding sequences. AP1 was originally identified as a transcription factor essential for the basal enhancer of the human metallothionein IIA and SV40 promoters (12) and was also found to be inducible by various stimuli, such as growth factors, cytokines, and UV irradiation (13, 14). The activity of AP1 is regulated by several mechanisms, including alteration in the expression of specific AP1 components, which may affect dimer formation and consequent affinity, as well as protein phosphorylation, which regulates transcriptional activity without changing the ability to bind to a target DNA sequence (reviewed in Ref. 15).
Our previous studies identified FER-1 as both an enhancer and an E1A-responsive element of the mouse ferritin H gene and suggested that members of the Jun and Fos families recognize the AP1-like element of FER-1. However, the specific proteins binding to both the element with an AP1-like sequence and the element with dyad symmetry within FER-1 have not been elucidated. Characterization of nuclear factors that act on the FER-1 enhancer element is therefore essential not only to delineating regulatory mechanisms in transcriptional control of the ferritin H gene but also to better understanding the mechanism of E1A-mediated transcriptional repression.
We report here that 1) JunD, FosB, and ATF1 are contained in the binding complex that recognizes the AP1-like sequence of FER-1; 2) JunD and FosB are able to activate FER-1 enhancer activity, and this activation retains E1A sensitivity; 3) the C-rich sequence in the dyad element of FER-1 is involved in binding of Sp1 and Sp3 transcription factors; and 4) recombinant ATF1 and Sp1 are able to bind the FER-1 element simultaneously, suggesting that transcription factors that bind to components of the FER-1 element act in concert to stimulate ferritin H transcription.
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MATERIALS AND METHODS |
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Cell Culture-- All mouse cell lines used in this study (NIH3T3 fibroblasts, F9 embryonal carcinoma cells, BNL CL.2 normal liver cells, and L929 fibroblasts) were grown in high-glucose Dulbecco's modified Eagle's medium (Life Technologies, Inc.) supplemented with 10% fetal bovine serum (Gemini Bioproducts) at 37 °C in a humidified 5% CO2 atmosphere.
Plasmids--
Most of the 5- ferritin H-CAT plasmids used in
this study were described previously (9). Briefly, pBluescript II
KS(
)
4.8-kb FHCAT contains 4.8 kb of the 5
-mouse ferritin H
enhancer/promoter region (16) fused to the chloramphenicol
acetyltransferase (CAT) gene in the pBluescript II KS(
) vector.
pBluescript II KS(
)
StuI/EcoRV FHCAT lacks
the 3.8-kb region containing FER-1 and was constructed by deletion of
the StuI (
4.13 kb)-EcoRV(
0.32 kb) fragment
from pBluescript II KS(
)
4.8-kb FHCAT. Two copies
(antisense-antisense orientation) of the 37-bp FER-1 element
(5
-CCATGACAAAGCACTTTTGGAGCCCAACCCCTCCAAA-3
) or three copies
(antisense-antisense-sense orientation) of the 16-bp AP1-like element
(5
-CCATGACAAAGCACTT-3
) of FER-1 were inserted into the
SmaI site (
0.22 kb) of pBluescript II
KS(
)
StuI/EcoRV FHCAT to construct
pBluescript II KS(
)
StuI/EcoRV,
SmaI/FER-1CAT and pBluescript II
KS(
)
StuI/EcoRV, SmaI/AP1-like
CAT, respectively. These CAT constructs were used for cotransfection
assays with mouse JunD and FosB expression plasmids (Fig. 3) as well as
for transfection in various mouse cell lines (Table I). Expression of
JunD and FosB was under the control of the SR
promoter in the
pcDEB
vector (17). An expression plasmid of mouse CREB, pHDmCREB
(18), was kindly provided by Dr. Gunther Schutz (German Cancer Research Center, Heidelberg, Germany).
Gel Retardation Assay-- The oligonucleotides used for gel retardation assays were purified from 20% acrylamide, 50% urea gel, followed by elution, Sephadex G25 column chromatography, and annealing according to standard procedures (19). Synthetic double-stranded oligonucleotides were end-labeled with T4 polynucleotide kinase. DNA sequences of the oligonucleotides with deletions and mutations in the FER-1 element are shown in Fig. 4. Isolation of nuclear extracts and binding reaction were carried out as described previously (9). In gel supershift experiments, 5-20 µg of nuclear extracts were incubated with 2 µg of antibodies for 2 h at 4 °C prior to incubation with oligonucleotide probes at room temperature for 15 min. All antibodies for gel supershift assays were purchased from Santa Cruz Biotechnology; anti-Jun family (D, sc44x), anti-c-Jun (N, sc45x), anti-JunB (N-17, sc46x), anti-JunD (329, sc74x), anti-Fos family (K25, sc253x), anti-c-Fos (4, sc52x), anti-FosB (102, sc48x), anti-fra1 (N-17, sc183x), anti-fra2 (Q-20, sc604x), anti-ATF1/CREB1 (25C10G, sc270x), anti-CREB (C-21, sc186x, and 240, sc58x), anti-ATF1(C41-5.1, sc243x), anti-Sp1 (PEP2, sc59x) and anti-Sp3 (D-20, sc644x). Recombinant human ATF1 and Sp1 were purchased from Santa Cruz Biotechnology and Promega, respectively. Binding reactions were carried out at 37 °C for 30 min in the presence of 1.25 ng of end-labeled 37-bp FER-1 probe, 100 µg of bovine serum albumin, 1 µg of poly(dI·dC) in 20 µl of total volume. Protein-DNA complexes were separated by 5% polyacrylamide gel electrophoresis at 4 °C.
DNA Transfection and CAT Assay-- Transfection of test plasmids into NIH3T3 cells was performed by DEAE-dextran method (8), and F9, BNL CL.2, and L929 cells were transfected by calcium phosphate precipitation method (20). CAT assay was carried out as described previously (8), and individual spots on silica gel plates were measured by PhosphorImager analyzer (model 445Si, Molecular Dynamics).
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RESULTS |
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FER-1 Is an Enhancer of the Ferritin H Gene in a Wide Variety of Cell Types-- The FER-1 element was initially identified using NIH3T3 mouse fibroblasts (9). To determine whether the FER-1 element also participates in ferritin H transcriptional regulation in other cell types, we performed transfection experiments in the normal liver cell line BNL CL.2, the transformed cell line L929, and the teratocarcinoma cell line F9 as well. As shown in Table I, the insertion of the FER-1 element into a ferritin H-CAT construct lacking the FER-1 region increased the CAT activity in these cell lines. Similar results were observed in TA1 mouse preadipocytes (data not shown). These results suggest that the FER-1 element functions as a general basal enhancer of the ferritin H gene in cell lines with widely different tissue origins.
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Complexes Bound to the AP1-like Element of FER-1 Contain JunD, FosB, and ATF1-- Our previous studies demonstrated that Jun and Fos family members are components of the binding complex in the AP1-like element of FER-1 (9). To identify the specific members of AP1 transcription factors bound to this element, we performed gel supershift experiments using antibodies specific to each member of the AP1 family. Nuclear extracts prepared from NIH3T3 cells were preincubated with either an antibody that broadly cross-reacts to the members of the Jun family or with specific antibodies against c-Jun, JunB, or JunD, followed by addition of a probe oligonucleotide with the consensus AP1 sequence (TGACTCA) or the AP1-like sequence of FER-1 (TGACAAA). As we observed previously, anti-Jun family antibody altered the mobility of the protein-DNA complexes derived from both probes (Fig. 1A, lanes b and g). We then tested the reactivity of antibodies specific to each Jun family member to the proteins bound to the consensus AP1 oligonucleotide. As shown in Fig. 1A, all antibodies induced an additional band with slower migration (lanes c-e), indicating that these three Jun family members exist in NIH3T3 cells and are able to bind to the consensus AP1 sequence. The same experiment was carried out with the AP1-like sequence of FER-1 as a probe (Fig. 1A, lanes f-j). In contrast to the results obtained with the consensus AP1 oligonucleotide, only anti-JunD antibody induced a reproducible supershift in the mobility of the binding complex (Fig. 1A, lane j), suggesting that among Jun family members, JunD is the major component of the AP1-like element binding complex.
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The AP1-like Sequence of FER1 Is Optimal for Basal Enhancer
Activity--
Selective members of the AP1 and ATF/CREB family
recognize a consensus AP1 binding sequence or a consensus CREB binding
sequence more strongly than related nucleotide sequences (21). This was true when the AP1-like sequence of FER-1 was changed to a consensus AP1
binding sequence, which increased total protein binding by approximately 6-fold in gel retardation assay (data not shown, but see
Fig. 1). We therefore asked whether substitution of the AP1-like
sequence of FER-1 for a consensus AP1 or CRE sequence is able to
increase the enhancer activity of the ferritin H gene. To address this
question, we introduced nucleotide changes into the AP1-like sequence
(TGACAAA) of FER-1 in pBluescript II KS()
4.8-kb FHCAT plasmid to
create a consensus AP1 site (TGACTCA,
4.8-kb AP1FHCAT) or a consensus
CRE site (TGACGTCA,
4.8-kb CREFHCAT). These CAT constructs were
cotransfected into NIH3T3 cells with pUC18 or p12SE1A, and CAT activity
in the cells was measured. As shown in Fig.
2, mutation of the AP1-like sequence of
FER-1 to either the consensus AP1 or CRE site failed to increase basal enhancer activity of FER-1 or responsiveness to E1A-mediated
repression. These results suggest that the participation of selective
members of the AP1 and ATF/CREB families in the binding complex of the FER-1 element is more important than the magnitude of total protein binding and that the AP1-like nucleotide sequence of FER-1 is optimal
for the enhancer activity of the ferritin H gene.
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JunD and FosB Activate the FER-1 Enhancer--
We next assessed
the functional involvement of JunD and FosB proteins in FER-1 enhancer
activity. For this purpose, the AP1-like element of FER-1 or the entire
FER-1 element was inserted into a ferritin H-CAT reporter gene lacking
the FER-1-containing 3.8-kb region (pBluescript
IIKS()
StuI/EcoRVFHCAT (9)). These constructs were cotransfected into F9 cells with mouse JunD and FosB expression plasmids. F9 cells were chosen for this assay because they have low AP1
activity (22), which would minimize the effect of endogenous AP1
activity on the expression of our CAT reporter plasmids. The ferritin
H-CAT construct lacking the FER-1 region expressed minimal CAT activity
in F9 cells even if JunD and FosB expression plasmids were
cotransfected (Fig. 3, columns
a-d). In contrast, insertion of three copies of the AP1-like
element of FER-1 by itself slightly increased the basal expression of
CAT, probably due to a minor amount of endogenous AP1 activity in F9
cells (Fig. 3, column e). The CAT activity was further
increased by cotransfection with JunD and FosB expression plasmids
(Fig. 3, column g), whereas transfection of a mouse CREB
expression plasmid failed to increase the CAT activity (data not
shown). Insertion of two copies of the entire FER-1 element (containing
the AP1-like element and the dyad element) in the same CAT vector by
itself further increased the basal expression of CAT (Fig. 3,
column i), as we observed previously (9), and this CAT
expression was similarly augmented by transfection of JunD and FosB
expression plasmids (Fig. 3, column k). In both cases,
cotransfection of E1A decreased the CAT activity stimulated by JunD and
FosB expression plasmids (Fig. 3, compare column g with
column h and column k with column l). These results support the concept that JunD and FosB participate in
FER-1 enhancer activity and that they are targets for transcriptional repression by E1A.
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A C-rich Sequence in the Dyad Element of FER-1 Represents the
Minimal Element Required for Protein Binding and Transcriptional
Activation--
Our previous results demonstrated that a mutation in
the dyad element that eliminates both dyad symmetry and the ability to bind nuclear factors decreased FER-1 enhancer activity (9). However,
the key nucleotide sequences in the dyad element required for protein
binding and the nature of nuclear factors binding to this element
remain unknown. To address these questions, we tested FER-1
oligonucleotides containing various partial sequences of the dyad
element for their ability to bind proteins in a gel retardation assay
(Fig. 4). Oligonucleotide probes
containing the AP1-like element alone (Fig. 4, AP1-like),
the AP1-like element plus a partial dyad sequence containing a deletion
of 15 nucleotides (FER-115) and the AP1-like element plus
a dyad sequence containing a deletion of 9 nucleotides
(FER-1
9) all gave rise to a similar retarded band. This
represented the interaction between AP1 transcription factors and the
AP1-like element, as judged by competition experiments demonstrating
competition by the AP1-like oligonucleotide and absence of competition
by the dyad (Fig. 4, FER-1
9). In contrast, reconstitution
of FER-1 by the addition of 9 nucleotides conferred the ability to
interact with additional proteins (see Fig. 4, FER-1, no
competitor), suggesting that the complete dyad sequence or at least the
3
portion of the dyad element including 5
-CCCTCCAAA-3
(compare
FER-1
9 with FER-1) is important for protein interaction. In fact,
mutations in this sequence (Fig. 4, mDyad-2) consistently caused the loss of ability to bind a set of proteins.
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Sp1 and Sp3 Bind to the C-rich Sequence of the FER-1 Dyad Element-- The middle of the dyad element has a C-rich sequence spaced by AA and T (Fig. 4; CCCAACCCCTCC), suggesting a potential Sp1 binding site (CCCGCCCC or related GC-rich sequences) (23). Based upon the results shown in Fig. 4, we anticipated that this C-rich sequence in the dyad element might play a role in protein binding. We therefore examined the possibility that Sp1 or Sp1-related factors may bind to the dyad element of FER-1. For this purpose, we performed gel retardation assays using the consensus Sp1 oligonucleotide as a probe or a competitor. As shown in Fig. 5A, the 22-bp dyad element of FER-1 and the 22-bp consensus Sp1 oligonucleotide (Promega) gave rise to a similar pattern of retarded bands, although the dyad element of FER-1 had a lower apparent affinity for nuclear proteins than the consensus Sp1 sequence (Fig. 5A, lanes a and g). In addition, the interaction between nuclear proteins and the dyad element of FER-1 was inhibited not only by the unlabeled dyad element (Fig. 5A, lanes b and c) or the complete FER-1 element (lane f) but to a further extent by the consensus Sp1 oligonucleotide in a dose-dependent fashion (lanes d and e). In the reverse experiment, the interaction between the nuclear proteins and the consensus Sp1 binding sequence was partially inhibited in a dose-dependent manner by excess unlabeled dyad oligonucleotide (Fig. 5A, lanes j and k) or the complete FER-1 element (lane l). Taken together, we conclude that the C-rich sequence in the dyad element of FER-1 contributes to the protein binding and that it binds Sp1 or Sp1-family proteins.
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Binding of Recombinant Proteins to the FER-1 Element-- We have previously demonstrated that the AP1-like element and the dyad element of FER-1 cooperatively activate the transcription of the ferritin H gene (9). To determine whether this synergy is due to the interaction of binding proteins to the two elements of FER-1, we tested the ability of purified recombinant proteins to bind to the FER-1 element. A gel retardation assay was performed using recombinant ATF1 and Sp1, both of which were detected in the binding complex of FER-1 in gel supershift experiments (Figs. 1C and 5B). In support of the supershift experiments, both recombinant proteins individually bound to the FER-1 element in a dose-dependent fashion (Fig. 6, lanes a-d). When ATF1 and Sp1 proteins were mixed together in the binding reaction, both were able to recognize the FER-1 element with no apparent effect of the binding of one protein on the affinity of the other (Fig. 6, compare lanes a and c with lane e). At higher concentrations of ATF1 and Sp1, a new complex with slower migration was detected (lane f). This complex was not affected by control rabbit IgG, but it disappeared following incubation with anti-ATF1 or anti-Sp1 antibody (data not shown), suggesting the formation of ternary complex between these two recombinant proteins and the FER-1 element. These results suggest that the two elements of FER-1 have the ability to bind the corresponding transcription factors simultaneously and that one transcription factor (e.g. ATF1) does not recruit the other (e.g. Sp1) to the FER-1 element.
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DISCUSSION |
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Ferritin H, a gene expressed in most tissues, is subject to
transcriptional control in response to a variety of endogenous and
exogenous stimuli, including oncogenes, cytokines, hormones, and
chemical inducers of differentiation (5, 7, 28-30). The 5 regulatory
region of ferritin H reflects this complex repertoire of responses. We
have identified two elements, FER-1 and FER-2, located approximately
4-kb 5
to the transcriptional start site of ferritin H. FER-2, located
at
4.7 kb, mediates tumor necrosis factor responsiveness through
binding of nuclear factor
B factors (31); FER-1, located at
4.1
kb, mediates basal enhancer activity through interaction with an
independent set of transcription factors, which we identify here. FER-1
is also a target of E1A-mediated transcriptional repression (8). FER-1
is composed of two elements: an AP1-like sequence followed by an
element with dyad symmetry (9). In the present study, we have
characterized specific transcription factors that interact with these
components of the FER-1 element. Binding proteins interacting with the
AP1-like element of FER-1 were identified as JunD, FosB, and ATF1 by
gel retardation assays using antibodies specific to AP1 and ATF/CREB
family members (Fig. 1).
Through genetic modification of the FER-1 sequence, we were able to
test whether this specific constellation of transcription factors was
in fact required for optimal enhancer activity of FER-1. When the 16-bp
AP1-like sequence in the FER-1 element containing TGACAAA was changed
to the consensus AP1 binding sequence TGACTCA, the affinity of total
protein binding was increased by approximately 6-fold in a gel
retardation assay (data not shown, but see Fig. 1). This was
accompanied by a change in members of the AP1 and ATF/CREB
transcription factors in the binding complex (Fig. 1). However, the
conversion of the AP1-like sequence of FER-1 into the consensus AP1
sequence in the 4.8-kb ferritin-HCAT did not further increase CAT
expression following transient transfection into NIH3T3 cells (Fig. 2).
The report that cAMP increases ferritin H transcription in rat thyroid
cells (29) and in rabbit smooth muscle cells (32) suggests that other
ATF/CREB family members may also participate in the mouse ferritin H
regulation through the FER-1 element. However, the conversion of the
AP1-like sequence into a consensus CRE sequence (TGACGTCA) did not
increase the FER-1 enhancer activity; rather, it caused it to decrease
(Fig. 2). These results suggest that the particular array of
transcription factors bound to FER-1 is optimal for transcriptional
activation of the ferritin H gene.
Using functional assays, JunD and FosB were shown to activate ferritin H enhancer activity in F9 cells (Fig. 3), whereas CREB failed to do so (data not shown), suggesting that these AP1 family proteins contribute to ferritin H transcription in vivo. However, it should be noted that transfection of a c-Jun expression plasmid alone (data not shown) or c-Jun plus c-Fos expression plasmids (9) was also able to stimulate the FER-1-CAT reporter plasmid in F9 cells despite the apparent lack of binding of c-Jun or c-Fos proteins to the AP1-like sequence of FER-1 (Fig. 1). Although we do not have a precise explanation for these results, a possibility is that overexpressed c-Jun and c-Fos may be able to bind weakly to the AP1-like element of FER-1 or recruit endogenous JunD, FosB, or ATF1 to form a dimer competent to recognize the AP1-like element of FER-1, or that they may transcriptionally induce JunD, FosB, or ATF1 expression. These mechanisms may serve to confer a flexibility to the transcriptional regulation of ferritin H that may come into play in different cell types or under different physiological conditions.
Our previous studies demonstrated that the AP1-like element of FER-1 by itself had moderate enhancer activity and required a second element with dyad symmetry for maximal enhancer activity (9). Analysis of the key nucleotide sequences in the dyad element essential for protein interaction presented here demonstrated that a C-rich sequence (AGCCCAACCCCTCC) in the dyad element plays a role in binding of nuclear factors (Fig. 4). This C-rich sequence has homology to the binding site of GC box binding factors, Sp1, or Sp1-related proteins. In gel retardation assays, we found that the binding of proteins to the dyad element was in fact strongly competed by the consensus Sp1 oligonucleotide (Fig. 5A), suggesting that the C-rich sequences in the dyad element of FER-1 bind GC box binding factors, including Sp1. Among the Sp1-related transcription factors so far described (33), we identified Sp1 and Sp3 as the transcription factors associated with the dyad element of FER-1 (Fig. 5B). In addition, our results indicated that dyad symmetry per se was not important for protein binding or enhancer activity of FER-1 (Table II).
Our results provide evidence for a cooperative activation of the
ferritin H enhancer by members of the AP1 and Sp1 transcription factor
families. Although Sp1 by itself has the ability to activate the
transcription of target genes, several lines of evidence have recently
indicated that Sp1 also interacts and functionally cooperates with
other transcription factors, such as nuclear factor B (34) on
composite binding elements of HIV-1 enhancer. Very recently, the
cooperativity of AP1 and Sp1 in the transcriptional activation of human
granulocyte-macrophage colony-stimulating factor (35), human leukocyte
integrin, CD11c (36), and human Pi class glutathione S-transferase (37) has been reported. Our results suggest
that this cooperative interaction extends to a broad range of both AP1
and Sp1 family members, including JunD and FosB, as well as Sp1/Sp3.
Although the mechanisms underlying these effects remain to be
clarified, it has been suggested that in the case of the CD11c
promoter, Sp1 binding facilitates the binding of AP1 (36). In contrast,
our gel retardation experiments indicated that the dyad element and the
complete FER-1 element (the AP1-like element plus the dyad element)
were equivalently effective as competitors for binding to an Sp1
element (Fig. 5A, lanes k and l). These results,
as well as experiments with recombinant transcription factors (Fig. 6),
suggest that the cooperative effect of AP1 and Sp1 family members in
ferritin H transcriptional activation may proceed via a mechanism that
does not involve recruitment of one transcription factor by the
other.
The effect of Sp1 and/or Sp3 on ferritin H transcription was evident as a substantial augmentation of enhancer activity when compared with that driven by AP1 alone (Fig. 3). However, the inhibition of FER-1 enhancer activity by E1A did not appear to target the binding activity of Sp1. Thus, E1A did not alter binding of Sp1/Sp3 to FER-1 or facilitate the replacement of Sp1 family members bound to FER-1 by alternative inhibitory family members (data not shown) as seen in the repression of the fibronectin gene by E1A, in which a negative regulator (G10BP) competes for the binding of Sp1 to the G-rich sequences in the promoter region and represses transcription (38). In contrast, our previous results have demonstrated that E1A decreases binding of AP1 family members to FER-1 (9). Further experiments will be required to address whether the transcriptional inhibitory properties of E1A reside solely in its ability to target AP1 or whether modulation of the transcriptional activation properties of Sp1 and/or Sp3 or of transcriptional adaptor proteins also plays a role in the complex response of the ferritin H gene to multiple environmental and intracellular signals. The role of FER-1 in ferritin H transcriptional activation in a variety of cell types (Table I) suggests that elucidation of mechanisms of interaction of these transcription factors will provide insight into transcriptional regulation of this critical component of iron metabolism.
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ACKNOWLEDGEMENTS |
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We thank Y. Nakabeppu and G. Schutz for providing us with Jun/Fos expression plasmids and a CREB expression plasmid, respectively. We thank J. Ninomiya-Tsuji for discussion and E. Roesch for oligonucleotide synthesis.
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FOOTNOTES |
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* This work was supported by Grant DK-42412 from the National Institutes of Health. Phosphoimaging analysis was performed in a facility supported by Grant CA12197 from the National Institutes of Health and Grant 9510-IDG-1006 from the North Carolina Biotechnology Center. DNA Synthesis was performed by Core Laboratory of the Comprehensive Cancer Center of Wake Forest University, which is supported in part by Grant CA-12197 from the National Institutes of Health.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 910-716-0232;
Fax: 910-716-0255; E-mail: ytsuji{at}bgsm.edu.
1 The abbreviations used are: bp, base pair; kb, kilobase; CAT, chloramphenicol acetyltransferase; CRE, cAMP response element; ATF/CREB, activating transcription factor/c-AMP response element binding protein.
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REFERENCES |
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