From the Division of Human Cancer Genetics, Dana Farber Cancer Institute, and the Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
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ABSTRACT |
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The RecT protein of Escherichia coli is a DNA-pairing protein required for the RecA-independent recombination events promoted by the RecE pathway. The RecT protein was found to bind to both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) in the absence of Mg2+. In the presence of Mg2+, RecT binding to dsDNA was inhibited drastically, whereas binding to ssDNA was inhibited only to a small extent. RecT promoted the transfer of a single-stranded oligonucleotide into a supercoiled homologous duplex to form a D (displacement)-loop. D-loop formation occurred in the absence of Mg2+ and at 1 mM Mg2+ but was inhibited by increasing concentrations of Mg2+ and did not require a high energy cofactor. Strand transfer was mediated by a RecT-ssDNA nucleoprotein complex reacting with a naked duplex DNA and was prevented by the formation of RecT-dsDNA nucleoprotein complexes. Finally, RecT mediated the formation of joint molecules between a supercoiled DNA and a linear dsDNA substrate with homologous 3'-single-stranded tails. Together these results indicate that RecT is not a helix-destabilizing protein promoting a reannealing reaction but rather is a novel type of pairing protein capable of promoting recombination by a DNA strand invasion mechanism. These results are consistent with the observation that RecE (exonuclease VIII) and RecT can promote RecA-independent double-strand break repair in E. coli.
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INTRODUCTION |
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In Escherichia coli, the major recombination pathway
requires the function of the RecA and RecBCD proteins (for reviews, see Refs. 1 and 2). The recombination and repair deficiencies in recB
recC mutants can be suppressed by two types of mutations called
sbcA and sbcB(sbcC) (3-5). The sbcA
mutations map on the cryptic Rac prophage and induce the expression of
the RecE and RecT proteins (for review, see Ref. 6). Recombination in
recB recC sbcA mutants occurs by what is called the RecE
pathway (7), which in many ways is similar to the bacteriophage Red
pathway (6). A distinctive property of the RecE pathway is that it promotes RecA-independent recombination of circular plasmids as well as
intramolecular recombination of linearized plasmid DNAs (8-11) and
also promotes RecA-independent double strand break repair
(DSBR)1 (12). These types of
recombination events have been shown to require functional
recE and recT genes (13, 14).
The recE gene product is an ATP-independent exonuclease, also called exonuclease VIII (15). Exonuclease VIII degrades preferentially linear duplex DNA in the 5' to 3' direction, yielding 5'-mononucleotides and also degrades single-stranded DNA (ssDNA) at low rates (16). The recT gene product was found to bind to ssDNA and to promote the renaturation of complementary ssDNA in an ATP-independent fashion (17). Also, the RecT protein in combination with exonuclease VIII was shown to promote homologous pairing and strand exchange between a circular ssDNA and a linear duplex DNA. In this reaction, exonuclease VIII degraded the linear duplex to expose ssDNA that was then annealed by RecT to a complementary region on the ssDNA circle. Subsequently, RecT promoted heteroduplex extension and partial strand exchange (18). This degradation/reannealing/strand exchange mechanism explains how the presence of RecE and RecT can render some types of recombination RecA-independent (6). However, in vivo evidence indicates that the ends of a linear duplex DNA may not be involved directly in the initial pairing event, and it was suggested that internal duplex-duplex initiation events could be promoted by the RecE pathway (11, 19). Also, it has been pointed out that DSBR, which clearly requires a pairing function, cannot occur simply by a degradation and reannealing mechanism (20). This suggests that recombination promoted by the RecE pathway involves pairing events more complex than annealing of ssDNA, possibly similar to those promoted by RecA (6). These pairing events could either involve additional unidentified protein factors or alternatively, they could be the result of a previously unrecognized RecT pairing activity.
In this paper, we investigate further the pairing activities promoted by RecT in vitro. We found that RecT can promote the invasion of a supercoiled DNA by either a homologous ssDNA or homologous single-stranded ends of a linear duplex, a DNA substrate similar to that generated by the RecE exonuclease. The joint molecules contain D-loops (displacement loops), supporting the idea that RecT could be involved directly in the initiation of DSBR in the RecE pathway. Our biochemical analysis of the DNA binding properties of RecT and the requirements for strand transfer indicate that RecT belongs to a novel class of DNA-pairing proteins.
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EXPERIMENTAL PROCEDURES |
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Strains, Plasmids, and Media--
E. coli strains
Novablue (endA1
hsdR17(rK12mK12+)
supE44 thi-1 recA1 gyrA96 relA1 lac[F'
proA+B+
lacIqZ
M15::Tn10]) and Novablue(DE3),
containing a T7 RNA polymerase gene inducible with isopropyl
-D-thiogalactopyranoside, were from Novagen. pRac31 is
pBR322 containing a fragment of the Rac prophage which carries a
wild-type recT gene (13). pUC18 and pUC19 are from the
laboratory collection, and pHV2900OB is a pUC18 derivative carrying a
2.27-kilobase pair DNA fragment inserted into the polylinker (21).
E. coli cells were grown at 37 °C in LB medium (22)
supplemented with 0.2% glucose. Ampicillin and kanamycin were added to
final concentrations of 100 and 30 µg/ml, respectively.
Transformations were carried out as described previously (22).
Enzymes and Proteins-- Restriction enzymes, T4 polynucleotide kinase, T4 DNA ligase, DNA polymerase Klenow fragment, and bovine serum albumin were from New England Biolabs. Bacteriophage T7 gene 6 exonuclease and T7 DNA polymerase (Sequenase version 2.0) were from U. S. Biochemical Corp. Proteinase K was from Boehringer Mannheim.
Oligonucleotides-- Synthesis of oligonucleotides was performed at the Molecular Biology Core Facility, Dana Farber Cancer Institute. Primers used to amplify RecT were T1 (5'-cgggatccagaaggaatatgcaaatgactaagcaac) and T2 (5'-acgcgtcgacggtgcattacaccgccaggc); regions complementary to the recT region are underlined. Oligonucleotides used in DNA binding and D-loop assays were 50 nucleotides long. Oligonucleotide 26 (5'-caccgtcaccgacttgagccatttgggaattagagccagcaaaatcaccag) corresponds to positions 2629-2579 of bacteriophage M13 wild-type. Oligonucleotide 34 (5'-ctatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtg) corresponds to positions 146-195 of pUC18. Oligonucleotide 35 (5'-cacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatag) is complementary to oligonucleotide 34.
Overexpression and Purification of RecT--
A 0.85-kilobase
pair DNA fragment containing the recT gene was amplified by
polymerase chain reaction from pRac31 with primers T1 and T2, and the
polymerase chain reaction product was digested with BamHI + SalI and inserted into pET24+ (Novagen) cleaved with the
same enzymes. The resulting plasmid, pRDK577, carried a wild-type recT gene (verified by DNA sequencing) under the control of
the T7 promoter. The RecT-overproducing strain, RDK3294, was obtained by transformation of pRDK577 into Novablue(DE3). RecT synthesis was
induced by the addition of isopropyl
-D-thiogalactopyranoside (1 mM final
concentration) to a log-phase culture of RDK3294 (OD650 = 0.8) and incubation for 4 h. RecT purification was performed as
described previously (17). A final step was added in the purification
procedure to remove the KPO4 buffer from the RecT preparation obtained from the hydroxylapatite column. The RecT fraction
(28 mg of protein) was loaded at 25 ml/h onto a PBE94 column (20 × 1 cm) equilibrated with buffer A (20 mM Tris, pH 7.5, 10 mM 2-mercaptoethanol, 0.1 mM EDTA, 10% w/v
glycerol) containing 0.1 M NaCl and eluted with an 80-ml
linear gradient of buffer A containing 0.1-1 M NaCl. The
fractions containing RecT were pooled, dialyzed against buffer A
containing 60% w/v glycerol and 100 mM NaCl, and stored at
20 °C. Analysis by SDS-polyacrylamide gel electrophoresis and
Coomassie staining indicated that the final RecT fraction (25 mg of
protein) was greater than 95% pure. Protein concentrations were
determined by using the Bio-Rad assay kit with bovine serum albumin as
a standard.
DNA Substrates--
All DNA concentrations are expressed in
nucleotide residues. Double-stranded oligonucleotides were obtained by
mixing complementary oligonucleotides in equimolar amounts (2 mM) and annealing them by incubation in a water bath at
95 °C for 5 min in buffer R (25 mM Tris-HCl, pH 7.5, 1 mM EDTA, 100 mM NaCl) followed by slow cooling
to room temperature over 12 h. The duplex oligonucleotide was
purified from unannealed oligonucleotides by high performance liquid
chromatography on a GEN-PAC FAX column (Waters, Milford, MA) using a
NaCl gradient (25 ml) from 0.3 to 1 M, in 10 mM
Tris-HCl, pH 8.0, 1 mM EDTA, run at 0.75 ml/min. Under
these conditions, only duplex DNA bound to the column and eluted as a
single peak at 0.67 M NaCl. Single- or double-stranded
oligonucleotides were 5'-end labeled with [-32P]ATP
(Amersham) and T4 polynucleotide kinase and then purified on a Sephadex
G-25 column (Boehringer Mannheim) to remove unincorporated label.
DNA Binding Assay-- Reaction mixtures (30 µl) contained 32P-labeled ssDNA or dsDNA (0.83 and 1.66 µM, respectively) in buffer T (20 mM Tris-HCl, pH 7.5, 100 µg/ml bovine serum albumin, and 0.5 mM dithiothreitol). The concentrations of NaCl, MgCl2, and RecT are indicated in the figure legends. The reaction mixtures were incubated 20 min at 37 °C and filtered by means of a double-filter system (23) through KOH-treated nitrocellulose (24) and DEAE-cellulose membranes (Schleicher & Schuell). For stability analysis, the RecT-DNA complexes were preformed in 240-µl volumes containing 25 mM NaCl, as described above. A 75-fold molar excess of unlabeled ss- or dsDNA was added to the preformed complex, and aliquots (20 µl) were taken at various time points, filtered, and rinsed with 100 µl of binding buffer at 4 °C, as described (23). Data were quantified with a PhosphorImager (Molecular Dynamics). All experiments were repeated at least three times.
D-loop Formation Assay-- Reaction mixtures (30 µl) contained 0.83 µM 32P-labeled single-stranded oligonucleotide, 25 mM NaCl, 20 mM Tris-HCl, pH 7.5, 100 µg/ml bovine serum albumin, 0.5 mM dithiothreitol, and RecT as indicated in figure legends. Mixtures were incubated for 20 min at 25 °C, and supercoiled DNA was added at the concentrations indicated in individual experiments. Mixtures were then incubated for an additional 30 min at 37 °C, and the reactions were stopped by the addition of 0.5 M EDTA, pH 8.0, 10% SDS, and 10 mg/ml proteinase K to the final concentrations of 50 mM, 0.2%, and 550 µg/ml, respectively. Incubation at 37 °C was continued for 20 min, and the mixtures were supplemented with 5 µl of tracking dye (0.25% bromphenol blue, 0.25% xylene cyanol, and 60% glycerol) and loaded on a 0.9% agarose gel in TAE buffer (40 mM Tris acetate, pH 8.0, 2 mM EDTA). Electrophoresis was performed for 150 min at 5 volts/cm at 25 °C. The gel was soaked in 300 ml of water containing 1 µg/ml ethidium bromide for 20 min, photographed, and soaked in 300 ml of 7% trichloroacetic acid for 30 min at 4 °C, neutralized in 200 mM Tris-HCl, pH 8.2, for 20 min, and dried onto Whatman 3MM paper for 3 h at 60 °C. Radiolabeled DNA species were then quantified using a PhosphorImager. Conditions for the reactions using DNA substrates resected with T7 gene 6 exonuclease were the same except that 19 µM resected pUC19 DNA (0.11 pmol) and 40 µM supercoiled pHV2900OB (0.12 pmol) were used as substrates. Electrophoresis was carried out in a 0.7% agarose gel for 16 h at 1.6 volts/cm at 25 °C.
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RESULTS |
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RecT Binds to ssDNA and dsDNA-- To examine the DNA binding properties of RecT, nitrocellulose filter binding assays were carried out with single- and double-stranded oligonucleotides, in buffer T containing 25 mM NaCl and no MgCl2. RecT was capable of binding to ssDNA and dsDNA (Fig. 1), and for both DNAs, binding was maximal after 5 min (data not shown). Surprisingly, RecT exhibited a greater affinity for dsDNA than for ssDNA because half-maximum binding was observed at a protein/DNA ratio of 1 RecT monomer/3.3 base pairs and 1 RecT monomer/1.6 bases, respectively. To investigate the effect of Mg2+, binding reactions were repeated in the same buffer containing 5 mM MgCl2 (Fig. 1). RecT binding to dsDNA was reduced dramatically (half-maximum binding at 8 RecT monomers/base pair), whereas binding to ssDNA was much less affected (half-maximum binding at 1 RecT monomer/1.2 bases).
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RecT Promotes D-loop Formation-- The results presented above revealed that RecT has a greater affinity and forms more stable complexes with dsDNA than with ssDNA. To test if RecT could promote a pairing reaction involving a fully duplex DNA, we monitored the invasion of a supercoiled DNA by a 32P-labeled ssDNA (50 nucleotides). The RecT-ssDNA complexes were preformed separately and then mixed with the supercoiled DNA substrate. The joint molecules, which are D-loops, were detected as the comigration of radioactivity with the supercoiled plasmid DNA. The kinetics of joint molecules formation is presented in Fig. 4. After about 30 min, the reaction reached a plateau corresponding to 33% of the supercoiled DNA being converted into D-loops. A slight increase in D-loop formation was observed reproducibly at 120 min (Fig. 4B), indicating that the reaction had not yet reached completion. Similar levels of D-loop formation were obtained with oligonucleotides homologous to several different loci in the supercoiled plasmid, indicating that D-loop formation is not specific for a particular DNA sequence (data not shown).
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dsDNA-RecT Complexes Cannot Pair with ssDNA-- In the experiments presented above, formation of joint molecules was obtained when ssDNA-RecT complexes were preformed before the addition of dsDNA, consistent with the idea that the RecT-ssDNA filament promoted pairing (26). To investigate further the role of the dsDNA-RecT complexes in joint molecule formation, dsDNA was preincubated with RecT, and the dsDNA-RecT complex was then mixed with uncoated ssDNA. Under these conditions, D-loops were not detected even after 60 min of incubation (data not shown). These results indicate that the RecT-dsDNA complex is incapable of promoting the pairing reaction. However, when RecT was added to a mixture of uncoated ss- and dsDNA substrates, weak but significant D-loop formation (1% of the dsDNA converted) was observed (data not shown). This suggests that RecT can promote pairing of ss- and dsDNA substrates but that the formation of RecT-dsDNA complex inhibits the pairing reaction.
To test this possibility further, the effect of RecT concentration on D-loop formation was examined. Varying amounts of RecT were preincubated with the ssDNA, and the reaction was initiated by adding dsDNA to the mixture. The results are presented Fig. 5. The amount of joint molecules formed increased with increasing concentrations of RecT and reached an optimum at between 0.3 and 0.43 µM RecT, corresponding to a ratio of about 1 RecT monomer/2.5 bases of ssDNA. This optimal pairing activity occurred at subsaturating concentrations of RecT, where 20-26% of the ssDNA is bound (see Fig. 1). At RecT concentrations exceeding 0.43 µM, D-loop formation diminished sharply, indicating that pairing was inhibited by an excess of RecT. One possible explanation for this inhibition is that in excess of the optimum concentration, enough RecT would be available to bind to the dsDNA substrate and inhibit the pairing reaction.
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RecT Mediates the Strand Transfer of the Single-stranded Ends of a DNA Duplex-- We have shown that RecT has a higher affinity for dsDNA than for ssDNA and that the RecT-dsDNA complexes inhibited pairing. Together, these findings raised the question of whether RecT-ssDNA complexes competent for strand transfer could be formed at the single-stranded ends of a duplex DNA that has been resected by an exonuclease. Such a resected duplex is assumed to be the DNA substrate to initiate recombination in the RecE pathway (6). To test this idea, pUC19 DNA was linearized by NarI and treated briefly with an exonuclease that degrades the 5'-strands. This linear duplex DNA with 3'-single-stranded tails (3'-ssDNA) was then preincubated with varying amounts of RecT, and supercoiled pHV2900OB was added to initiate the reaction. The formation of joint molecules (Fig. 7) increased with the amount of RecT and reached an optimum at 0.67 µM RecT, where 8% of the supercoiled DNA was trapped in joint molecules. Increasing the RecT concentration further resulted in a decrease in joint molecule formation. The formation of joint molecules was not observed if the exonuclease treatment of the linear pUC19 DNA was omitted (data not shown), consistent with the known requirement of RecT for ssDNA ends (18). As a control, a DNA substrate having 29 base pairs of heterology at each single-stranded end was generated by cleaving pUC19 with XbaI, at the center of the polylinker region (which is not present in pHV2900OB (21)), followed by limited degradation of the 5'-strands with T7 gene 6 exonuclease. No joint molecule formation was observed with this DNA substrate (data not shown), indicating that homologous single-stranded tails are required for the formation of D-loops. These results show that RecT can promote pairing of a supercoiled DNA with a DNA duplex having homologous single-stranded tails, indicating that RecT filaments can form on those single-stranded tails. Interestingly, strand transfer occurred at subsaturating concentrations of RecT (1 RecT monomer/14 base pairs), where one would predict that most of the RecT protein would be bound to dsDNA (see Fig. 1).
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DISCUSSION |
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To investigate the pairing reactions promoted by RecT, we examined the interactions between RecT and DNA, using a nitrocellulose filter binding assay. We found that RecT bound to ssDNA as well as to dsDNA but with an apparent higher affinity for dsDNA than for ssDNA. Binding to dsDNA was inhibited by the addition of Mg2+. Previous filter binding studies only revealed RecT binding to ssDNA, and previous electron microscopy studies revealed RecT-ssDNA helical nucleoprotein filaments but only sparse internal binding of RecT with dsDNA (17, 26). These differences can be explained by the fact that the previous binding studies were carried out in the presence of high salt and high Mg2+, conditions which strongly inhibited binding of RecT to dsDNA (see Figs. 1 and 2). Interestingly, RecT binding to dsDNA was enhanced by the addition of 25 mM NaCl, whereas binding to ssDNA was not. Analysis of the decay of RecT-ssDNA and RecT-dsDNA complexes revealed that RecT displayed at least two modes of binding to DNA. Each complex was composed of two forms, an unstable complex with a half-life < 1 min, and a very stable complex with a half-life > 120 min. Under our experimental conditions, more of the bound DNA was of the stable form with dsDNA (45%) than with ssDNA (22%). Although the role of these different complexes is not known, the results of competition experiments suggest that the stable form of the RecT-ssDNA complexes is the most active species involved in pairing and could possibly be the only active species.
A striking feature of RecT binding to DNA is its sensitivity to Mg2+. The addition of 5 mM MgCl2 to the reaction drastically reduced the binding of RecT to dsDNA and, to a much smaller extent, its binding to ssDNA. This observation explains why previous binding studies, carried out in the presence of 13 mM MgCl2, did not detect RecT binding to dsDNA (17). Electron microscopy studies revealed that in the presence of MgCl2, RecT monomers assemble into doughnut-shaped oligomers containing 7 or 8 monomers and rod-like structures but that in the absence of MgCl2, no oligomer was formed (26). Together these results suggest that optimum binding to DNA is achieved when RecT is only in a monomer form, suggesting in turn that RecT oligomers might not be proficient for binding, especially to dsDNA.
The results presented in this study show that RecT protein can efficiently transfer a ssDNA into a homologous duplex DNA in vitro, leading to the formation of a joint molecule containing a D-loop. Strand transfer did not require any high energy cofactor; it occurred at 0, 1, and 2.5 mM MgCl2 and was inhibited completely by the presence of 5 mM MgCl2. Optimum strand transfer was achieved when RecT was preincubated with ssDNA at a ratio of 1 RecT monomer/2.5 bases of ssDNA and subsequently reacted with uncoated dsDNA, indicating that pairing is mediated by a RecT-ssDNA complex. RecT-dsDNA complexes were unable to participate in pairing with uncoated ssDNA or with RecT-ssDNA complexes. However, both strand transfer and RecT binding to dsDNA displayed the same sensitivity to salt and Mg2+ concentrations, suggesting that the capacity of RecT to bind dsDNA likely plays some role in strand transfer. In contrast, RecT binding to dsDNA was not required for the annealing of complementary ssDNA because this reaction occurred efficiently in the presence of high MgCl2 concentrations (17, 18).2 Taken together, these results suggest that RecT promotes joint molecule formation by a mechanism that is different from annealing. Finally, RecT can mediate D-loop formation between a supercoiled dsDNA and a linear dsDNA having homologous 3'-single-stranded tails, indicating that, despite its higher affinity for dsDNA, RecT is able to polymerize on the single-stranded tails, making them invasive. Indeed, this type of binding was observed previously by electron microscopy under conditions in which RecT would not bind to dsDNA (26).
Our understanding of homologous pairing reactions comes mostly from the extensive studies of the E. coli RecA protein (for a review, see Ref. 27) and similar proteins such as the bacteriophage T4 UvsX protein (28), Saccharomyces cerevisiae Rad51 (29), and human Rad51 (30). This class of homologous pairing proteins requires a high energy cofactor and Mg2+ to form a nucleoprotein filament on the ssDNA which then promotes synapsis with the homologous duplex partner. RecT protein also forms nucleoprotein filaments with ssDNA which then catalyze the strand transfer reaction. However, unlike RecA, RecT performs strand transfer without ATP and in the absence of Mg2+ as well as at low concentrations of Mg2+. Other ATP-independent DNA-pairing proteins have been shown to promote pairing and strand exchange in vitro, such as the bacteriophage T7 gene 2.5 protein (31), E. coli RecO protein (32), and S. cerevisiae Sep1 protein (33, 34). However, in all cases, the addition of Mg2+ was required for the reaction. This feature of RecT, which could be related to its higher affinity for dsDNA than for ssDNA, suggests that RecT differs from these other DNA strand transfer proteins. Alternately, the inhibition of strand transfer by high Mg2+ concentrations could be similar to the situation with RecA protein where presynaptic filament formation occurs more efficiently at low concentrations of Mg2+ compared with high concentrations of Mg2+, presumably because of the inhibitory effect of secondary structure in ssDNA at high Mg2+ concentrations (35). It also suggests that the level of unbound Mg2+ in the cell could regulate the RecT-promoted strand transfer reactions. Unfortunately, no accurate measurement of free Mg2+ levels is currently available (36 and references therein), although they are generally thought to be at low levels in the range on 1 mM where RecT promotes D-loop formation.
Genetic evidence indicates that RecT is required for DSBR in recBC sbcA strains (14). This RecT-promoted DSBR is independent of RecA function and occurs by a conservative mechanism (12, 37). Here, we provide in vitro evidence that RecT can promote the invasion of a DNA duplex by a ssDNA to form a D-loop, which is the predicted initiation step in DSBR models (38, 39). Therefore, our results indicate that RecT can initiate recombination not only by promoting DNA-reannealing and heteroduplex exchange (18) but also by a DNA strand invasion mechanism, thus providing an explanation for the RecA independence of DSBR in recBC sbcA strains. Double strand breaks have been shown to induce recombination-dependent DNA replication in recBC sbcA strains where the RecE pathway of recombination is activated (40). In this background, recombination-dependent DNA replication was shown to depend on a functional RecE pathway and partially on RecA function. It was suggested that the residual recombination-dependent DNA replication activity observed in the absence of RecA could be attributed to the pairing activity of RecT (40). Our finding that in vitro, RecT promotes efficient D-loop formation with DNA substrates similar to those that the RecE exonuclease would generate strongly supports this interpretation. Taken together, these results suggest that RecT can promote D-loop formation in vivo. The biological importance of this process is underlined by mounting evidence suggesting that D-loop formation can be used not only to repair double-strand breaks but also to generate active replication forks (for review, see Ref. 41).
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ACKNOWLEDGEMENTS |
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We are grateful to Pascale Bertrand, Hernan Flores-Rozas, Gerry Marsischky, Tiehua Ni Abhijit Datta, and Marie Francoise Noirot-Gros for helpful discussions and critical reading of this manuscript and to all other members of our laboratory for enthusiastic support. We also are very grateful to Era Cassuto for constructive comments on this manuscript.
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FOOTNOTES |
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* This work was supported by National Institutes of Health Grant GM26017 (to R. D. K.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Present address: INRA, CRJJ, 78352 Jouy-en-Josas, France.
§ Present address and to whom correspondence should be addressed: Ludwig Institute for Cancer Research, University of California San Diego School of Medicine, CMME3080, 9500 Gilman Dr., La Jolla, CA 92093-0660.
1 The abbreviations used are: DSBR, double strand break repair; D-loop, displacement loop; ssDNA, single-stranded DNA; dsDNA, double-stranded DNA.
2 P. Noirot, unpublished results.
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REFERENCES |
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