From the Department of Pharmacology, School of Medicine, University of South Alabama, Mobile, Alabama 36688
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ABSTRACT |
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As a first step in determining what cellular
processes are regulated by the calcium-modulated protein S100A1 isoform
in neurons, the effects of ablated S100A1 expression on neurite
organization and microtubule/tubulin levels in PC12 cells were
examined. A mammalian expression vector containing the rat S100A1
cDNA in the antisense orientation with respect to a cytomegalovirus
promoter was constructed and transfected into PC12 cells. Indirect
immunofluorescence microscopy confirmed decreased S100A1 protein levels
in all three stable transfectants (pAntisense clones) that expressed
exogenous S100A1 antisense mRNA. In response to nerve growth
factor, pAntisense clones extended significantly more neurites than
control cells (4.01 ± 0.16 versus 2.93 ± 0.16 neurites/cell). This increase in neurite number was accompanied by an
increase in total -tubulin levels in untreated (4.0 ± 0.6 versus 1.76 ± 0.4 ng of
-tubulin/mg of total
protein) and nerve growth factor-treated pAntisense clones (4.15 ± 0.4 versus 2.04 ± 0.5 ng of
-tubulin/mg of
total protein) when compared with control cells. At high cell
densities, pAntisense clones exhibited a significant decrease in
anchorage-dependent growth. In soft agar, pAntisense clones
formed significantly more colonies (153 ± 8%) than control cells
(116 ± 5%). However, the pAntisense soft agar colonies were
significantly smaller than those observed in control cells (40.6 ± 3.0 versus 59.5 ± 1.2 µm). These data suggest
that cell density inhibits both anchorage-independent and
-dependent growth of pAntisense clones. In summary,
ablation of S100A1 expression in PC12 cells results in increased
tubulin levels, altered neurite organization, and decreased cell
growth. Thus, S100A1 may directly link the cytoskeleton and calcium
signal transduction pathways to cell proliferation.
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INTRODUCTION |
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The S100 protein family is a group of calcium-binding proteins
that exhibit a high degree of conservation in amino acid sequence, secondary structure, and genomic organization (1-3). To date, there
are 18 members of the S100 family, 13 of which are clustered in region
q21 of human chromosome 1 (see Refs. 4 and 5). A new nomenclature that
reflects the genomic organization of these proteins has recently been
adopted, and S100 is now designated S100A1 and S100
is designated
S100B (6). S100A1 and S100B were the original members of this family
and have been implicated in a diverse group of cellular functions
including cell-cell communication, cell growth, cell structure, energy
metabolism, contraction, and intracellular calcium signal transduction
(see Refs. 1 and 3).
Because each S100 monomer contains two EF-hand high affinity calcium binding domains, they have been included in the S100-troponin-calmodulin superfamily of calcium-modulated proteins. These proteins have no known enzymatic activity and function by modulating the activity of other proteins termed target proteins. Calmodulin appears to be the "workhorse" in calcium signaling and to be responsible for generating the main events triggered by changes in intracellular calcium levels. This view is consistent with the observations that calmodulin is present in all cells; that it is highly conserved among species, phyla, and even kingdoms; that it is not a family of proteins; and that there is signal amplification in calmodulin-regulated events. In contrast, S100 proteins are excellent candidates for providing cell type specificity and acting as modulators, rather than instigators of calcium signal transduction. S100 proteins are not ubiquitously expressed and exhibit very specific patterns of expression, are a diverse multi-component family, are expressed at significantly lower levels than calmodulin, and often act stoichiometrically rather than catalytically. Although S100 modulation of some target proteins is calcium-dependent, other S100 effects are calcium-independent, suggesting that these proteins may have important functions at resting intracellular calcium levels.
Analysis of intact cells in which the expression of S100 proteins has been ablated or up-regulated has been an extremely useful approach in identifying cellular processes that are regulated by S100 family members. Using an antisense RNA to inhibit S100B expression in C6 glioma cells, Selinfreund and co-workers (7) observed three phenotypic changes: 1) a more flattened morphology, 2) a more organized actin filament network, and 3) a slower growth rate. Lakshmi and co-workers (8) used retinoic acid and melanocyte-stimulating hormones to alter S100A4 (originally designated mts1) expression in melanoma cells and observed a direct relationship between S100A4 and depolymerized tubulin levels. More recent studies by Takenaga and co-workers (9) using antisense RNA to down-regulate S100A4 expression in lung carcinoma cells demonstrated that reduced S100A4 expression is associated with suppression of metastatic potential. Masiakowski and Shooter (10) used a sense expression vector to induce S100A6 (originally designated 42C) expression in PC12 cells and observed neurite extension without inhibition of cell proliferation in the absence of NGF.1 All of these effects were observed without manipulation of intracellular calcium levels and are consistent with the view that S100 proteins perform important functions at resting intracellular calcium levels. Furthermore, these studies directly implicate S100 proteins in the regulation of cell growth and cytoskeletal organization.
Previous studies have indirectly implicated another member of the S100
family, S100A1, in regulating cytoskeletal organization. Like S100A6,
S100A1 expression is up-regulated in NGF-treated PC12 cells that are
extending neurites (11). In addition, a number of S100A1 target
proteins are essential structural elements of the cytoskeleton
including tubulin, protein, intermediate filaments, caldesmon, and
myosin (see Ref. 1). Furthermore, several S100A1 target proteins are
associated with the cytoskeleton including aldolase,
glyceraldehyde-3-phosphate dehydrogenase, and phosphoglucomutase (see
Refs. 1 and 11). The expression of both S100A1 and the
microtubule-associated
protein in neuronal cells further suggests
that S100A1 regulates microtubule assembly in neuronal cells.
Rat pheochromocytoma cells (PC12 cells) are an extensively used model
system for studying neuronal cell differentiation and signal
transduction. Furthermore, antisense/sense approaches have been used in
PC12 cells to examine the in vivo functions of a wide
variety of neuronal proteins including (12), GAP-43 or neuromodulin
(13-16), the
subunit of Go (17),
calmodulin-dependent protein kinase II (18), annexin II
(19), and the plasma membrane calcium ATPase pump (20). To determine if
S100A1 regulates cytoskeletal organization in neuronal cells, we have
examined the effects of ablated S100A1 expression on
-tubulin levels
in PC12 cells as well as the number and length of neurites extended by
PC12 cells in response to NGF. We observed increases in
-tubulin
levels in PC12 cells that do not express S100A1. Furthermore, PC12
cells that do not express S100A1 extended more neurites per cell in response to NGF. These cytoskeletal changes were accompanied by reductions in anchorage-dependent and anchorage-independent
growth that were density-related. The fact that three members of the S100 protein family, S100B, S100A6, and now S100A1, modulate growth and
cytoskeletal organization suggest that these proteins link the
cytoskeleton and calcium signal transduction pathways to cell growth.
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EXPERIMENTAL PROCEDURES |
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Construction of the pAntisense Expression Vector-- To construct the pAntisense expression vector, a plasmid containing the rat S100A1 cDNA (21) was PCR-amplified using M13 reverse primer (CLONTECH, Palo Alto, CA) and a gene-specific primer containing an engineered XbaI restriction enzyme site (5'-GGGTCTAGAGACCCTCATCAATGT-3'). After digestion with XbaI and HindIII and gel purification, the fragment was ligated into gel-purified XbaI-HindIII-digested pCMV expression vector (22). The resulting DNA construct (Fig. 1A) was transformed into Escherichia coli, and ampicillin-resistant colonies were screened by restriction mapping and PCR analysis. DNA sequence analysis (23) of the 5' region was performed to verify the orientation of the insert DNA.
Cell Culture and Transfection-- PC12 cells were the generous gift of Dr. Jonathan Scammell (University of South Alabama, Mobile, AL) and grown as described previously (11). PC12 cells (1.7 × 106 cells in 0.8 ml) were transfected by electroporation at 400 V using a Bio-Rad Gene PulserTM apparatus (Bio-Rad), equipped with a 960-microfarad capacitor and 0.4-cm gap electrodes. A plasmid containing the neomycin resistance gene under the control of an SV40 promoter (InVitrogen, San Diego, CA) was cotransfected with the S100A1 pAntisense plasmid or plasmid containing no insert DNA. Cells were plated in 100-mm dishes, and 48 h later stable transfectants were selected by treatment with 1 µg/ml G418 (Life Technologies, Inc.). After 3 weeks, single colonies were transferred to 96-well microtiter plates and expanded.
Selection of Clones Expressing S100A1 Antisense
mRNAs--
Stable transfectants were screened for the presence of
exogenous S100A1 antisense mRNA using reverse transcriptase and PCR analysis. Total RNA was prepared from PC12 cells using RNA-Stat60 (Tel-Test "B", Inc., Friendswood, TX). First strand cDNA was
prepared using a Perkin-Elmer (Roche Molecular Systems, Inc.,
Branchburg, NJ) Gene-AmpTM RNA PCR kit. A reaction mix containing 1 µg total RNA, 50 mM KCl, 10 mM Tris-HCl, pH
8.3, 1 mM each dATP, dTTP, dCTP, and dGTP, 5 mM
MgCl2, 20 units of RNase inhibitor, 2.5 µM oligo(dT)16, and 50 units of murine leukemia virus reverse
transcriptase was incubated at room temperature for 10 min,
40-42 °C for 1 h, and 90-95 °C for 5 min, and then placed
in ice. PCR amplification reactions consisted of 4 µl of the reverse
transcriptase reaction, 50 mM KCl, 10 mM
Tris-HCl, pH 9.0, 0.1% Triton X-100, 1.25 mM MgCl2, 0.2 mM each dATP, dTTP, dCTP, and dGTP,
and 2.5 units of Taq DNA polymerase (Promega Corp., Madison,
WI). Oligonucleotide primers whose sequences correspond to the 3'
region of the S100A1 cDNA coding sequence
(5'-CAGTTTGTAGCAGGTCTTTCAGCT-3') and the human growth hormone
termination signal of the pCMV vector (5'-CTGGAGTGGCAACTTCCAAG-3') were
used for detection of the pAntisense S100A1 mRNA. The integrity of
all RT reactions was confirmed by amplification of a 541-bp -actin
fragment corresponding to nucleotides 144-685 of the
-actin cDNA using gene-specific oligonucleotide primers
(5'-GTGGGGCGCCCCAGGCACCAG-3' and 5'-CTCCTTAATGTCACGCACGATTTC-3'). The
integrity of the PCR was confirmed by using purified plasmid DNA,
rather than RT reactions as template.
Indirect Immunofluorescence Microscopy-- PC12 cells were grown on glass coverslips and processed for indirect immunofluorescence microscopy as described previously (11). A commercial anti-S100A1 mouse monoclonal antibody (1:20 dilution) (Sigma) was used as a primary antibody for S100A1 staining. Primary antibody binding was visualized with a fluorescein isothiocyanate-conjugated goat anti-mouse IgG (1:20 dilution) (Boehringer Mannheim). Slides were examined on a Leitz microscope equipped with epifluorescence optics and fluorescein filters. Images were recorded on Tri-X film (ASA 1600) with a 40× neofluor objective. To allow for fluorescence intensity comparisons between images, coverslips for control and pAntisense clones were processed simultaneously in each experiment. In addition, all images were recorded using a standard exposure time of 45 s. Furthermore, in each experiment, all images were developed and printed simultaneously using identical settings. Three independent experiments were performed using cells plated on different days.
Measurement of Neurites-- Quadruplicate 35-mm plates were seeded at low density, and 48 h later two plates were fed with medium only and two plates with medium containing 10 ng/ml NGF (Collaborative Research, Inc., Waltham, MA). Forty-eight hours later, five random fields were photographed from each plate using a Leitz microscope equipped with phase optics and a 20× objective. Individual/discernible cells were scored for number of neurites and neurite length on study prints (final magnification, ×645). Individual processes were measured in millimeters and converted to micrometers during analysis, with processes 2 µm or greater in length scored as neurites. Between 9 and 20 different fields consisting of 120-200 cells were scored for each clone. Each experiment was repeated using cells plated on different days. The data were expressed as the mean ± the standard error of the mean. An ANOVA (GraphPad, San Diego, CA) was used to determine the statistical significance of measured differences.
Anchorage-dependent Growth Analysis-- Anchorage-dependent growth was assessed by plating approximately 95,000 cells in triplicate 60-mm dishes and determining the cell number using a hemacytomer at 0, 24, 48, 72, and 192 h after plating. An ANOVA (GraphPad) was used to determine the statistical significance of measured differences.
Anchorage-independent Growth Analysis-- Anchorage-independent growth was assayed in 0.15% agarose with a 0.8% agarose underlay. Approximately 50,000 cells were plated in triplicate 60-mm dishes. Cells were fed 7 days later and photographed 14 days later on a Leitz microscope using a 10× objective. Colony number and colony size were measured on two randomly chosen photographs from each plate, and the average of all measurements was used as the colony number/colony size for that clone. Each experiment was repeated using cells plated on different days. Within each experiment, the number of colonies formed by the parental PC12 cells was set to 100% and the percent colonies formed by control and pAntisense clones expressed as the number of colonies/number of PC12 colonies × 100. The data were expressed as the mean colony size and colony number (percent) for the six determinations (three pAntisense clones × two experiments). An ANOVA (GraphPad) was used to determine the statistical significance of measured differences.
Quantitation of -Tubulin Levels--
Duplicate 100-mm plates
were fed with media only or media containing 10 ng/ml NGF
(Collaborative Research, Inc.). Forty-eight hours later, the plates
were rinsed three times in phosphate-buffered saline and cell extracts
prepared as described previously (24). After rinsing in Extraction
Buffer (EB) (0.1 M PIPES, 0.1 mM
MgSO4, 2 mM EGTA, 0.1 mM EDTA, pH
7.0) plates were incubated in 500 µl of EB containing 0.1% Triton
X-100 at room temperature. After 8 min, the cells were mechanically
removed and this cell homogenate used for determining total
-tubulin. A portion of the cell homogenate was centrifuged for 15 min at 10,000 × g and 4 °C, the supernatant was
removed, and the pellet was resuspended in 250 µl of EB. The supernatant was used for determining soluble
-tubulin levels and the
pellet for polymerized
-tubulin levels. Quantitative analysis of
cell extracts was performed as described by Hanemaaijer and Ginzberg
(25) using serial dilutions of each sample and an
-tubulin standard
(Cytoskeleton, Denver, CO). Samples were applied to a nitrocellulose
membrane using a Bio-Rad dot blot apparatus. After blocking in
Tris-buffered saline (200 mM NaCl, 50 mM Tris,
pH 7.4) containing 0.5% Tween 20 and 10% nonfat dry milk, blots were
incubated with anti-
-tubulin monoclonal antibody (1-333 dilution;
Sigma) followed by an 125I-labeled goat anti-mouse
secondary antibody (ICN Biomedicals, Inc., Irvine, CA). Bound
radioactivity was determined using a Bio-Rad GS250 Molecular Imager and
Phosphor Analyst Software. The nanograms of
-tubulin in each dot
were determined by extrapolation from the
-tubulin standard curve.
All dilutions within the linear range of the standard curve were
averaged to determine the nanograms of
-tubulin in each sample,
which was normalized to the milligrams of total protein (26). An ANOVA
(GraphPad) was used to determine the statistical significance of
measured differences.
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RESULTS |
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Isolation of pAntisense Clones--
In generating the clones used
in this study, approximately 200 stable transfectants were isolated. Of
the 17 stable transfectants screened for expression of the exogenous
pAntisense S100A1 mRNA, three clones (A1, A2, and A3) exhibited the
predicted 265-bp PCR product (Fig.
1B). Our inability to detect a
PCR product in the remaining 14 clones was not due to technical
problems with the RT or PCR reactions, as all clones exhibited a 500-bp
fragment using primers for endogenous -actin mRNA. Indirect
immunofluorescence microscopy was used to determine if the three clones
that expressed the exogenous S100A1 antisense mRNA also exhibited
decreased S100A1 protein levels. Clones A1, A2, and A3 exhibited a
S100A1 staining intensity indistinguishable from that of secondary
antibody only and significantly less than that observed in parental
PC12 cells or cells transfected with vector containing no insert DNA
(Fig. 2). Altogether, these results
demonstrate that the A1, A2, and A3 clones (pAntisense clones) have
S100A1 protein levels that are significantly less than parental PC12
cells and are an appropriate model system for studying S100A1-regulated
processes.
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NGF Responsiveness of pAntisense Clones-- Previous studies demonstrating that S100 proteins regulate microtubule assembly in vitro (see Ref. 1) and increased S100A1 levels in PC12 cells treated with NGF (11) suggest that S100A1 regulates microtubule polymerization/depolymerization. To test this hypothesis, phase contrast microscopy and morphometric techniques were used to examine the NGF-induced neurite extension in pAntisense clones. To maximize the length of neurites extended, the effects of NGF were assayed at low confluence. As shown in Fig. 3, the morphology of the three pAntisense clones was identical to that of control cells in the absence and presence of NGF. Furthermore, there was no difference in the NGF dose-response curve for the pAntisense clones and control cells (data not shown). These results demonstrate that reduced S100A1 levels do not inhibit or promote NGF-induced neurite extension in PC12 cells.
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Tubulin Levels in PC12 Cells--
The increased number of neurites
in NGF-treated pAntisense clones suggests that S100A1 may regulate the
level of polymerized/unpolymerized tubulin in PC12 cells. To test this
hypothesis, an immunodot assay was used to quantitate -tubulin
levels in PC12 cell total homogenates as well as in fractions
containing polymerized or unpolymerized tubulin. Western blot analysis
of PC12 cell homogenates confirmed that the commercial antibody used
for the immundot experiments recognized a single protein species (data
not shown). As shown in Fig.
5A, pAntisense clones
contained significantly more
-tubulin protein (4.0 ± 0.6 versus 1.76 ± 0.4 ng/mg of total protein) than control
cells. Furthermore, NGF treatment did not significantly alter the
-tubulin levels in control or pAntisense clones. It should be noted
that the total
-tubulin levels in this study are approximately
2-fold higher than those previously reported for PC12 cells (24), and
this may account for the small processes that we observe in cells which
are not treated with NGF, as well as the fact that we achieve maximal
neurite extension in our cells after 48 h whereas other
investigators routinely use 7 days. In summary, ablation of S100A1
expression in PC12 cells results in an NGF-independent increase in
-tubulin levels.
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Growth Properties of pAntisense Clones-- Because changes in the cytoskeleton are often associated with altered growth properties and members of the S100 family have been shown to regulate cell growth, the anchorage-dependent and anchorage-independent growth properties of the pAntisense clones were assayed. Although the growth properties of pAntisense clones at low densities were not different from control cells, at high densities pAntisense clones exhibited a significant decrease in anchorage-dependent growth (Fig. 6). Anchorage-independent growth was also altered in the pAntisense clones (Fig. 7). When all colonies larger than a single cell were scored, the three pAntisense clones formed significantly more colonies (153 ± 8%) in soft agar than control cells (116 ± 5%). Because cells were initially plated at equivalent numbers, this increase in colony number cannot be attributed to an increase in the number of cells plated. The increase in colony size cannot be attributed to experimental variability either, as the colony number in each experiment was normalized to the number of colonies formed by the parental PC12 cells which was set at 100%. These results demonstrate increased anchorage-independent growth in PC12 cells that do not express S100A1. Significant differences in colony size were also detected with the pAntisense clones forming colonies that were significantly smaller (40.6 ± 0.03 µm) than cells transfected with vector only and the parental PC12 cell line (59.5 ± 1.2 µm). Because control and pAntisense cells were similar in size, the smaller pAntisense colony size can be attributed to fewer cells in the colony. These results demonstrate that as cell density increases, anchorage-independent growth of pAntisense clones is reduced. Altogether, these results suggest that S100A1 modulation of cell growth is both complex and density-dependent.
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DISCUSSION |
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This study reports the first information regarding the function of
the calcium-binding protein S100A1 in intact cells. Decreased S100A1
levels in PC12 cells resulted in an increase in tubulin levels and the
number of neurites extended in response to NGF. In theory, cells that
are assembling more processes would have higher levels of structural
components such as microtubules. In fact, PC12 cells that do not
express S100A1 did have increased levels of -tubulin. We also report
that decreased S100A1 levels in PC12 cells alter cell growth in a
density-dependent manner. Although the reductions in S100A1
initially increase anchorage-independent growth, as cells become more
dense, the net result in both anchorage-independent and
anchorage-dependent growth is a reduction in cell number. These results suggest that S100A1 expression promotes cell growth and
eliminates some aspect of contact-inhibited cell growth. One model that
accounts for our results would be that S100A1 directly modulates
tubulin/microtubule levels in PC12 cells and that the effects on cell
growth and calcium homeostasis are indirect effects, which occur as a
consequence of the changes in tubulin/microtubules. This model is
consistent with previous studies, which have identified tubulin and
tubulin-associated proteins as S100A1 target proteins (see Ref. 1).
However, S100A1 has been shown to regulate the activity of a number of
growth regulatory proteins in vitro, including the tumor
suppressor p53 (28). Thus, it is possible that S100A1 interacts with
multiple target proteins and directly regulates both
tubulin/microtubule levels and cell growth. Additional experiments will
be needed to determine if S100A1 directly or indirectly regulates various cellular processes.
The results that we observed in PC12 cells are in accordance with previous in vitro studies on S100 target proteins. First, previous studies have reported that S100A1 proteins interact with numerous in vitro target proteins, suggesting that S100A1 will regulate multiple diverse processes in cells and that S100A1 regulation of these processes can be modulated at multiple sites. Consistent with these studies are our observations that S100A1 regulates multiple cellular processes in PC12 cells and that the effects of S100A1 on these processes can change under various conditions such as cell density. Second, the fact that S100A1 regulates most in vitro target proteins in a stoichiometric rather than catalytic fashion suggests that the effects of S100A1 on cellular process will be modulatory and not all-or-none. As predicted, in PC12 cells we observed modulatory rather than all-or-none effects. Third, the fact that some S100A1 target proteins are regulated in a calcium-independent manner suggests that altered S100A1 expression would have significant effects on cell phenotype even in the absence of agents that raise intracellular calcium levels. This view is supported not only by our observations with S100A1, but also by studies on S100B (7), S100A4 (8), and S100A6 (10). Because we have observed a small decrease in resting intracellular calcium levels in pAntisense cells, we cannot completely rule out the possibility that the effects which we observed are calcium-dependent.2 Nonetheless, we can say that these processes are regulated by S100A1 in absence of the large changes in intracellular calcium usually associated with activation of the calcium signaling cascade. Additional studies will be needed before the target proteins involved in and calcium dependence of S100A1 regulation of tubulin/microtubule levels, neurite organization, and cell growth in PC12 cells can be ascertained.
The results presented in this study also provide new insights into the relationship between S100 family members. First, all of the S100 proteins that have been studied in intact cells have been documented to regulate the cytoskeleton and cell growth (7-10). These observations suggest that S100 family members have redundant functions. However, when one examines an individual cell type, each family member appears to regulate different aspects of these processes. For example, in PC12 cells, S100A6 initiates neurite extension in response to NGF (10) whereas S100A1 determines how many neurites will be extended. Furthermore, S100A6 does not alter cell growth whereas S100A1 does. Additional studies on other S100 family members will be required before the universality of nonredundant function for S100 family members can be established.
In summary, this study provides direct evidence that S100A1 regulates tubulin/microtubule levels, neurite organization, and cell growth in PC12 cells. In fact, S100A1 may be a molecule that directly links the cytoskeleton and cell growth. The observations that increases in microtubule stability can reverse neuronal cell death associated with Alzheimer's disease (29) as well as neurotoxic agents such as acrylamide and carbon disulfide (30), glutamate (31), and N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (32) suggest that S100A1 may be a suitable pharmacological target for treating neurological disorders. S100A1 antagonists may also be useful agents for the treatment of cancers such as renal carcinoma because these cancers express high levels of S100A1 (see Ref. 1), and this study demonstrates that decreased S100A1 expression/function is associated with decreased growth rates.
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ACKNOWLEDGEMENTS |
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We thank Alexander Landar for technical assistance and J. Chessher for assistance in preparing the figures.
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FOOTNOTES |
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* This work was supported by Grant NS 30660 from the National Institutes of Health, Grant BIO-920038 from the National Science Foundation, and a grant from the Pine Family Foundation, Inc.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
To whom correspondence should be addressed: MSB 3130, Dept. of
Pharmacology, University of South Alabama, Mobile, AL 36688. Tel.:
334-460-7056; Fax: 334-460-6798; E-mail: dzimmer{at}jaguar1.usouthal.edu.
1 The abbreviations used are: NGF, nerve growth factor; PCR, polymerase chain reaction; RT, reverse transcriptase; bp, base pair(s); ANOVA, analysis of variance; EB, Extraction Buffer; PIPES, 1,4-piperazinediethanesulfonic acid.
2 D. B. Zimmer, E. H. Cornwall, P. D. Reynolds, and C. M. Donald, unpublished observation.
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REFERENCES |
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