From the European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69012 Heidelberg, Federal Republic of Germany
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ABSTRACT |
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In polarized Madin-Darby canine kidney (MDCK) cells, sorting of membrane proteins in the trans-Golgi network for basolateral delivery depends on the presence of cytoplasmic determinants that are related or unrelated to clathrin-coated pit localization signals. Whether these signals mediate basolateral protein sorting through common or distinct pathways is unknown. The cytoplasmic domain of the cation-dependent mannose 6-phosphate receptor (CD-MPR) contains clathrin-coated pit localization signals that are necessary for endocytosis and lysosomal enzyme targeting. In this study, we have addressed the function of these signals in polarized sorting of the CD-MPR. A chimeric protein, made of the luminal domain of the influenza virus hemagglutinin fused to the transmembrane and cytoplasmic domains of the CD-MPR was stably expressed in MDCK cells. This chimera (HCD) is able to interact with the AP-1 Golgi-specific assembly proteins and is detected on the basolateral plasma membrane of MDCK cells where it is endocytosed. Deletion analysis and site-directed mutagenesis of the cytoplasmic domain of the CD-MPR indicate that HCD chimeras devoid of clathrin-coated pit localization signals are still transported to the basolateral membrane where they accumulate. A HCD chimera containing only the transmembrane domain and the 12 membrane-proximal amino acids of the CD-MPR cytoplasmic tail is also found on the basolateral membrane but is unable to interact with the AP-1 assembly proteins. However, the overexpression of this mutant results in partial apical delivery. It is concluded, therefore, that the basolateral transport of this chimera requires a saturable sorting machinery distinct from AP-1.
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INTRODUCTION |
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The plasma membrane of polarized cells can be divided into two distinct domains, apical and basolateral, which exhibit different protein and lipid compositions. The generation and the maintenance of these domains require a continuous supply of newly synthesized components. In MDCK1 cells, newly synthesized membrane proteins destined for the basolateral or the apical surface are sorted in the trans-Golgi network (TGN) and packaged into distinct transport vesicles (1). Vesicular transport from the TGN to the apical or basolateral plasma membrane domains are mechanistically different. Although the docking/fusion of transport vesicles with the basolateral plasma membrane relies, like many transport steps, on the presence of the common fusion machinery involving NSF/SNAP proteins, the apical delivery appears to be independent of these proteins (2). More recent studies have indicated that nonpolarized cells also make use of two types of transport intermediates for the delivery of membrane proteins to the plasma membrane, one dependent on the presence of NSF/SNAP proteins and another independent of these complexes (3). Thus, polarized and nonpolarized cells have fairly similar overall organization of membrane traffic within the secretory pathway.
Thus far, two distinct features have been shown to determine sorting to the apical domain: first, the glycosylphosphatidylinositol anchor of membrane proteins (4, 5) and second, the mannose-rich core part of N-glycans present in the luminal domain of proteins (6). Many studies have now illustrated that sorting of membrane proteins to the basolateral plasma membrane is determined by the presence of specific, dominant protein determinants in their cytoplasmic domains (reviewed in Ref. 7). Extensive mutagenesis has uncovered two types of sorting motifs for basolateral delivery. First, there are those related to signals for clathrin-coated pit localization, which either rely on a key tyrosine residue, like those found in the LDL receptor (proximal determinant) (8), the vesicular stomatatis virus G protein (9), and lysosomal membrane glycoproteins (10), or on a di-leucine motif, like in the IgG Fc receptor (11). Second, there are basolateral targeting signals that are unrelated to determinants for clathrin-coated pit localization. Examples can be found in the LDL receptor (distal determinant) and in the poly-IgA receptor (8, 12, 13). Interestingly, the same (or a very closely related) basolateral sorting signal can mediate the recycling of endocytosed membrane proteins from endosomes back to the plasma membrane (14, 15). The similarities between the determinants responsible for endocytosis, basolateral sorting, and plasma membrane recycling suggest that these processes are extremely related and involve similar sorting machineries that remain to be characterized.
In addition to sorting membrane proteins destined for the apical or basolateral domains in the TGN, the polarized MDCK cells must also sort their newly synthesized lysosomal hydrolases bound to the mannose 6-phosphate receptors (MPRs). Previous studies have shown that one of the two MPRs, the mannose 6-phosphate/insulin-like growth factor II receptor (Man-6-P/IGF II), traffics within the basolateral domain of MDCK cells because it can be detected on the basolateral membrane of these cells (16). In nonpolarized cells, the endocytosis of this receptor relies on a tyrosine-based motif, whereas that of the other MPR, the cation-dependent mannose 6-phosphate receptor (CD-MPR), requires a weak tyrosine-based motif and a dominant motif containing two phenylalanine residues (17). On the other hand, efficient lysosomal enzyme targeting requires the presence of a di-leucine-based motif present at the carboxyl terminus of both MPRs (18-20). In addition, the signals required for efficient endocytosis of the MPRs contribute, although weakly, to efficient lysosomal enzyme targeting (19). The MPRs and their bound lysosomal enzymes are known to be sorted in the TGN via clathrin-coated vesicles. The first step in the formation of these transport intermediates is the interaction of the AP-1 Golgi-specific assembly proteins with TGN membranes. The MPRs are part of the membrane components that permit the efficient recruitment of AP-1 on membranes (21-23), a process regulated by the small GTPase ARF-1 (24). In the case of the CD-MPR, specific determinants in its cytoplasmic domain, in particular a casein kinase II phosphorylation site are required for high affinity interaction of AP-1 with TGN membranes (25).
In this study, we have investigated the sorting of the CD-MPR in polarized MDCK cells. For this, we have stably expressed a chimeric protein made of the luminal domain of the influenza virus hemagglutinin (HA) fused to the transmembrane and cytoplasmic tail of the CD-MPR. This HCD chimeric protein traffics within the basolateral domain and can be detected at the basolateral surface. Mutations of the different sorting signals proposed to mediate the interaction of the CD-MPR tail either with the Golgi-specific assembly proteins AP-1 or its plasma membrane counterpart AP-2 do not affect the basolateral delivery of the corresponding HCD chimeras. Truncations of the cytoplasmic domain indicated that a sorting determinant, unrelated to motifs necessary for clathrin-coated pit localization, is present in the membrane-proximal part of the CD-MPR cytoplasmic domain or the transmembrane domain, which confers basolateral targeting. This determinant, neither supports the AP-2-dependent endocytosis nor triggers the recruitment of AP-1 on membranes. This strongly suggests that an additional sorting machinery that recognizes signals unrelated to those mediating clathrin-coated pit localization could be responsible for basolateral targeting of membrane proteins in MDCK cells.
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EXPERIMENTAL PROCEDURES |
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Cell Culture-- MDCK cells (strain II) were grown as described (26). PA317 amphotropic retrovirus packaging cells (27) were maintained in MEM supplemented with 10% fetal calf serum, 4 mM glutamine, and antibiotics. All experiments, unless otherwise indicated, were performed with MDCK cells grown on 24.5-mm diameter, 0.4-mm pore size transwell units (Costar, Cambridge, MA). The polycarbonate filters were seeded with 0.5 to 1 × 106 cells each, and cell monolayers were used for experiments 4 days after plating.
HA and HA-MPR Constructs--
All manipulations of DNA were
performed essentially as described in Ref. 28. To optimize expression
of the chimeric proteins a Kozak consensus sequence was introduced into
the cDNA encoding HA. Two complementary oligonucleotides with the
sequences 5-CAAGCTTGCCGCCACCATGG-3
and 5
-CCATGGTGGCGGCAAGCTTGGTAC-3
were ligated between the KpnI and MscI sites of
plasmid pBHA (29) to create pBD16. To form pBD17 (wtHA), pBD16 was
cleaved with HindIII and BamHI and the short
fragment encoding the complete influenza virus hemagglutinin protein
was cloned between EcoRI and BamHI sites of the
retroviral vector pLXSN (30) after making the HindIII and
EcoRI ends flush with Klenow. The construction of a chimeric
gene, HCD, consisting of the luminal domain of the influenza virus HA
fused to the transmembrane and cytoplasmic domains of the small MPR has
been described previously (29). The chimeric gene HCD and all mutants
derived thereof were inserted in pBD17 between the SalI and
BamHI sites, thereby fusing the 5
end of HA, containing the
Kozak consensus sequence, to all chimeric genes. The cytoplasmic domain
truncation mutants (HCD-
5 (pBD23), HCD-
17 (pBD20), and HCD-
55
(pBD26); see Fig. 1) and the substitution mutants (HCD-
55,A-1
(pBD28) and HCD-A13A18A45 (pBD19)) were generated by
oligonucleotide-directed mutagenesis of the wild type mouse CD-MPR
cDNA (31) using the method of Kunkel (32). For the truncation
mutants the codons for the CD-MPR cytoplasmic tail residues 63 (His)
and 51 (Gln) were changed to stop codons. In the case of the
substitution mutants, the last residue (Tyr) of the transmembrane
domain and the cytoplasmic tail residues Phe13,
Phe18, and Tyr45 were changed to alanines. The
truncation mutant HCD-
23 (pBD25) was generated by ligation of two
complementary oligonucleotides with the sequences 5
-GCATGATTGG-3
and
5
-GATCCCAATCAT-3
between the PstI and BamHI
sites of HCD, introducing a stop codon at Tyr45 in the
cytoplasmic tail of the CD-MPR. Mutant HCD-
55 (pBD26) and
55,A-1
(pBD30) were constructed in a similar way using oligonucleotides with
the sequences 5
-ATGGAGCAGTGAG-3
and 5
-GATCCTCACTGCTCCATTCCC-3
. This
double-stranded DNA fragment was inserted between the BstXI and BamHI sites of HCD and HCD-A-1, respectively, creating a
stop codon at residue 13 (Phe) of the cytoplasmic domain.
Stable Expression in MDCK Cells-- Infectious virus was generated for each of the constructs from the plasmid form of the retroviral vector by transient transfection of the packaging cells PA317 essentially as described (30). Virus-containing medium harvested from PA317 cells was used to infect MDCK cells. Stably transfected MDCK cells were selected in medium containing 0.8 mg/ml G418 (Life Technologies, Inc.) and cloned using glass cylinders. Cells expressing the protein of interest were identified by immunofluorescence. At least two independent clones expressing the highest levels of the respective chimeric protein were used for further experiments. All transfected cell lines used were fully polarized as judged by methionine uptake (basal/apical ratio greater than 4:1) (33) and secretion of an endogenous glycoprotein complex (34). Prior to experiments the tightness of monolayers was assessed with [3H]inulin (Amersham Corp.) (35). All experiments were performed using cell lines of passage numbers 5 through 10 after cloning.
Cell Surface Transport Assay--
MDCK cells grown on Costar
Transwell units were rinsed with warm PBS++ (PBS containing
0.9 mM CaCl2 and 0.5 mM
MgCl2) and starved for 60 min in MEM lacking cysteine and
methionine (Select Amine-kit; Life Technologies, Inc.) containing 0.35 g/liter sodium bicarbonate, 20 mM Hepes, pH 7.3, and 0.5%
BSA (MEM-BSA). Cells were then pulse labeled in a wet chamber for 20 min at 37 °C from the basolateral side with labeling medium (MEM-BSA
supplemented with 2 mCi/ml Expre35S35S (1000 Ci/mmol, 10 mCi/ml) NEN Life Science Products). One set of filters was
washed three times with cold PBS++ and placed on ice in
MEM-BSA; the other sets of filters were chased at 37 °C in MEM-BSA
with a 100-fold excess methionine and cysteine. At the end of the chase
cells were washed with cold PBS++ and placed on ice in
MEM-BSA. Subsequent steps were performed at 4 °C. Cells were washed
once with MEM-BSA and incubated either from the apical or basolateral
side with a 1:500 dilution of an hemagglutinin antiserum (monoclonal
antibody H269, generous gift of J. Skehel) in MEM-BSA for 90 min on a
rocking platform. In some experiments the antibody was included in the
apical or basolateral chase medium and allowed to bind for another 90 min on ice. The excess unbound antibodies were removed over 30 min by
three washes with MEM-BSA and one wash with PBS++, filters
were cut out of the holder, and cells were lysed in the presence of an
excess of unlabeled protein. (For preparation of unlabeled lysates a
cell line overexpressing the wild type HA was grown to confluency on
10-cm culture dishes. Cells from one dish were lysed on ice in 2.5 ml
of B1 (50 mM Tris, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS containing freshly added protease inhibitor mixture (2 mg/ml leupeptin, 2 mg/ml aprotinin, 1 mM benzamidine, 1 mM phenylmethylsulfonyl
fluoride)). The lysate was cleared by centrifugation for 5 min at
12,000 × g, and 1 ml of supernatant was used to lyse
labeled cells.) The lysate was centrifuged in an Eppendorf microfuge
for 5 min to remove debris. An aliquot (one-tenth of the lysate) was
supplemented with additional antibody (anti-HA, mAb H269) and incubated
overnight at 4 °C, and total labeled protein was isolated by the
addition of protein A-Sepharose. The second aliquot (nine-tenths of the
lysate) was frozen in liquid nitrogen and stored overnight at
70 °C. Labeled protein that had appeared on the cell surface and
thus had bound antibody was precipitated by the addition of protein
A-Sepharose. Precipitates were washed three times with B1, twice with
B2 (50 mM Tris, pH 7.2, 100 mM NaCl, 2 mM EDTA, 0.1% Triton X-100, 0.5% SDS, 0.5%
deoxycholate), twice with B3 (50 mM Tris, pH 7.2, 500 mM NaCl, 2 mM EDTA, 0.1% Triton X-100), and
once with B4 (50 mM Tris, pH 7.2, 100 mM NaCl,
2 mM EDTA). Finally, proteins were released from the beads
by boiling in Laemmli sample buffer and analyzed by SDS-PAGE on a 10%
polyacrylamide gel (36). The band intensities were calculated using a
PhosphorImager (Molecular Dynamics, Sunnyvale, CA), and the amount of
protein transported to the cell surface was expressed as the percentage
of the total immunoprecipitated protein. In some experiments cells were
pretreated with 10 mM butyric acid (Sigma) 12 h prior
to labeling to induce transcription of stably transfected cDNA
constructs (8).
Sucrose Velocity Gradient Centrifugation-- MDCK cells grown on 60-mm plastic dishes to subconfluency and treated with 10 mM NH4Cl 12-16 h prior to the experiment to reduce the endogenous level of lysosomal proteases were metabolically labeled essentially as described above except that cells were labeled for 1 h and chased for 3 h. After labeling cells were lysed, and an aliquot of the lysate was analyzed by sucrose gradient centrifugation as described (37). Gradient fractions were immunoprecipitated with anti-HA and analyzed by SDS-PAGE and fluorography.
Internalization Assay Based on Surface Biotinylation-- Internalization rates of CD-MPR chimeras were determined by the surface biotinylation assay described in Ref. 38, except that MESNa was used for stripping of the cell surface biotin. Biotinylated CD-MPR chimera were detected by Western blotting with 125I-labeled streptavidin. Signals were quantified using a PhosphorImager.
AP-1 Recruitment--
HeLa cells were grown on coverslips in
-MEM supplemented with 10% fetal calf serum, 10 mM
Hepes, 2 mM glutamine, 100 units/ml penicillin, and 100 µg/ml streptomycin. The cells were washed in medium devoid of serum
and then incubated for 30 min with a recombinant virus that expresses
the T7 polymerase gene (39). After washing the cells in medium
supplemented with 5 mM hydroxyurea, the
N-[1(2,3-dioleoyloxy)propyl]-N,N,N-trimethyl-ammonium-methylsulfate reagent (Boehringer Mannheim) was used to transfect the cells with the
indicated constructs (HCD and HCD-
55) cloned in pGEM1 vector,
following the manufacturer's instructions. After 1 h, the cells
were washed and allowed to express for 2-3 h in medium supplemented
with 5 mM hydroxyurea. Pulse-chase experiments performed in
parallel indicated that similar levels of HCD and HCD-
55 were expressed under those conditions and transported through the secretory pathway with similar kinetics (data not shown). Cells were then fixed
for 20 min with 3% paraformaldehyde, permeabilized with 0.1% Triton
X-100 for 5 min, and subsequently incubated with the monoclonal 100/3
anti-
-adaptin antibody (kindly provided by E. Ungewickell) and a
rabbit polyclonal anti-HA antibody for 30 min at room temperature. The
bound antibodies were detected with fluorescein isothiocyanate or Texas
Red-conjugated secondary antibodies (Dianova).
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RESULTS |
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In MDCK cells, the Man-6-P/IGF II receptor traffics between the TGN and the endocytic organelles of the basolateral domain and is found on the basolateral plasma membrane (16). To study the transport of the CD-MPR in these cells, we have generated MDCK clones stably expressing chimeric proteins made of the luminal domain of the influenza virus HA, an apically sorted membrane glycoprotein, fused to the transmembrane and cytoplasmic domain of this receptor (Fig. 1) and performed pulse-chase experiments followed by cell surface immunoprecipitations to measure the appearance of these chimeric proteins on the apical or the basolateral domain.
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HA/CD-MPR Chimeras Are Normally Transported through the Secretory
Pathway and Are Present on the Basolateral Membrane of MDCK
Cells--
The normal transport of HA to the cell surface depends on
its proper folding and trimerization (37). Because modifications in the
transmembrane and cytoplasmic domains of HA can affect its normal rate
of transport (40, 41) we determined whether the HA/CD-MPR chimera (HCD
construct) is properly folded and transported through the secretory
pathway. We first performed classical pulse-chase experiments on MDCK
cells expressing either the wild type HA or the HCD and
immunoprecipitated these labeled proteins using a monoclonal antibody
directed against the luminal domain of HA. After a 20-min pulse, the HA
and the HCD were found in low molecular weight, immature forms that
were rapidly converted upon a chase into higher molecular weight,
mature forms reflecting the conversion of high mannose to complex type
oligosaccharides (Fig. 2). These results
indicate that the HCD chimera moves efficiently through the secretory
pathway with similar rates as the HA (t1/2 30 and 25 min, respectively). Such pulse-chase experiments were also performed with the HCD-
55, which harbors a 55-amino acid-long truncation of the CD-MPR tail (Fig. 2). The rate of transport of this
mutant was slightly slower than that of HCD (t1/2
45 min), indicating that the deletion of the CD-MPR tail only
moderately affects the transport of the corresponding chimera. The
other mutants used in this study were transported through the secretory pathway with similar rates as that of HCD-
55 (data not shown). The
oligomeric state of HCD and HCD-
55 were analyzed by centrifugation of detergent extracts on sucrose density gradients (37). After the
pulse, HCD and HCD-
55 immunoprecipitated from the different fractions distributed in a single peak, as the monomeric HA (Fig. 3). After a chase period, however, both
chimeric proteins with complex type sugars were recovered in denser
fractions, showing a similar profile as the mature trimeric HA. A
slightly higher percentage of the HCD-
55 remains in the lighter
density fractions in comparison with HA or HCD following a 3-h chase.
This suggests that the rate of oligomerization of the trucation mutant
is slightly slower than that of HA or HCD. Nevertheless, these results
show that both chimeric proteins acquire the same trimeric conformation as HA.
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Mutations of Endocytosis Sorting Signals Do Not Affect Basolateral
Transport of HA/CD-MPR Chimeras--
To decipher the determinants
important for sorting to the basolateral domain, we introduced several
deletions and point mutations in the CD-MPR cytoplasmic tail (Fig. 1).
The mutations were designed to affect the sequences known to be
important for intracellular trafficking of the CD-MPR. We first deleted
the carboxyl-terminal di-leucine motif alone (construct HCD-5) or
together with the adjacent casein kinase II phosphorylation site
(HCD-
17), shown to be critical for efficient lysosomal enzyme
targeting (18, 25). Table I shows that after a 40-min chase, 80% of
these mutant proteins present on the cell surface were still detected on the basolateral plasma membrane. Pulse-chase experiments followed by
cell surface immunoprecipitation indicated that the mutant proteins
were vectorially transported from the Golgi complex to the basolateral
domain (data not shown). The endocytosis of the CD-MPR is mediated by
two independent endocytosis motifs, a dominant determinant containing
phenylalanine 13 and 18 and a weak determinant containing the tyrosine
45 (17). We therefore replaced these three critical amino acids in the
HA/CD-MPR chimera by alanine residues (construct HCD-A13A18A45).
Indeed, these mutations significantly reduced the endocytosis rate of
the chimera by 60% (Table I). However, the sorting of this mutant was
not affected, and 90% of the cell surface protein was still found on
the basolateral plasma membrane. Therefore, it appears that neither the
endocytosis motifs nor the sequence important for lysosomal enzyme
delivery are essential for basolateral transport when mutated
individually.
Basolateral Sorting of HCD-55 Is Saturable--
Signal-mediated
sorting of membrane proteins in the secretory pathway of MDCK cells can
be saturated by overexpressing these membrane proteins. This has been
observed for the newly synthesized lgp 120 (10) or LDL receptor (8). To
test the possibility that basolateral sorting of the most truncated HCD
construct was signal-mediated and could become saturated, we selected
MDCK clones highly expressing the HCD-
55,A-1 construct (
7-fold
overexpression compared with HCD) and performed pulse-chase experiments
followed by cell surface immunoprecipitation. Fig.
5 shows that high levels of expression of
this mutant protein resulted in a significant missorting to the apical
domain when compared with lower expression levels. Thus, there is a
clear correlation between overexpression of this mutant and apical
delivery. The appearance on both plasma membrane domains occurred at
similar initial rates, strongly suggesting that the missorting of this
truncation mutant occurred in the trans-Golgi network. These results
suggest that the 12-amino acid-long sequence adjacent to the CD-MPR
transmembrane domain and/or the CD-MPR transmembrane domain contains a
determinant for basolateral transport and that the corresponding
sorting machinery could become saturated upon overexpression of this
chimera.
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HCD-55 Tail Does Not Interact with AP-1--
In nonpolarized
and polarized cells, the MPRs are sorted from the TGN via the
Golgi-specific assembly proteins AP-1-dependent pathway.
The recruitment of AP-1 on membranes can be triggered by the expression
of the MPRs (21-23). To test the possibility that the HCD-
55 could
still be sorted along an AP-1-dependent pathway, we
overexpressed HCD-
55 as well as HCD and monitored the recruitment of
AP-1 by immunofluorescence. Several established cell lines, such as
baby hamster kidney and Chinese hamster ovary cells, have been recently
shown to contain apical and basolateral cognate routes from the TGN to
the plasma membrane (3). These experiments were performed in the
nonpolarized HeLa cells, which most likely exhibit such cognate routes.
Fig. 6 shows that HeLa cells
overexpressing HCD exhibit, as expected, an increase in AP-1 staining
in the perinuclear region when compared with mock transfected cells.
However, AP-1 staining in cells overexpressing similar amounts of
HCD-
55 was indistinguishable from that of mock transfected cells.
Quantitation of these experiments indicated that expression of HCD
induced a 2-fold increase in AP-1 binding, whereas overexpression of
HCD-
55 remained without any effect (Fig.
7). We conclude from these results that
the HCD-
55 chimera is unable to recruit AP-1 on Golgi membranes.
Therefore, its basolateral targeting in MDCK cells does not involve the
AP-1-dependent pathway and may require an additional
sorting machinery.
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DISCUSSION |
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We have expressed in the MDCK cells chimeric proteins made of the transmembrane and cytoplasmic domain of the CD-MPR fused to the luminal domain of the apically directed hemagglutinin to study CD-MPR trafficking in polarized cells. We show that 1) the chimeric protein traffics within the basolateral domain, 2) the mutagenesis of determinants related to clathrin-coated pit localization signals in the CD-MPR tail does not affect its appearance on the basolateral membrane, 3) an additional sorting determinant is present in the cytoplasmic domain and/or the transmembrane domain of CD-MPR that mediates basolateral transport, and 4) this basolateral sorting determinant is not efficiently recognized by the Golgi-specific AP-1 assembly proteins and therefore may involve a different sorting machinery.
Our previous work in nonpolarized cells has shown that HA/MPR chimeras colocalize with the endogenous MPRs, indicating that the information contained within these chimeric proteins is sufficient to specify their intracellular localization (29). We have now expressed these HA/CD-MPR chimeras in polarized MDCK cells. Cell surface immunoprecipitation experiments revealed that more than 90% of the cell surface chimera is present at the basolateral plasma membrane indicating that this protein traffics within the basolateral domain of MDCK cells. This result agrees with that of Breuer and co-workers (44), who showed that the CD-MPR is predominantly present on the basolateral plasma membrane of MDCK cells. Thus, both the CD-MPR and the Man-6-P/IGF II receptor (16) traffic within the same cellular domain of MDCK cells. This HCD chimera is able to trigger the recruitment of the AP-1, Golgi-specific assembly proteins on membranes. Therefore, it is likely that its appearance on the basolateral membrane reflects its efficient sorting to endosomes along the AP-1-dependent pathway followed by the recycling of a fraction of this chimeric protein back to the basolateral plasma membrane.
To identify the minimal determinant required for this basolateral
sorting, we first mutagenized the signals in the CD-MPR cytoplasmic
domain known to be important for its endocytosis (phenylalanine 13 and
18 and tyrosine 45), efficient lysosomal enzyme targeting (carboxyl-terminal di-leucine motif), and high affinity binding of AP-1
on membranes (the casein kinase II phosphorylation site adjacent to the
carboxyl-terminal di-leucine motif). The re-expression of CD-MPR
mutants in MPR-negative fibroblasts has indicated that the mutation of
either the carboxyl-terminal di-leucine motif alone or the endocytosis
motif alone (phenylalanine 13 and 15 and tyrosine 45) does not affect
the efficient AP-1 recruitment (25). Consistent with this notion, the
same mutations introduced in the HCD chimera do not affect its
basolateral delivery. This suggests that the protein is still able to
follow the AP-1-dependent pathway in MDCK cells. The most
striking finding is that mutations in the CD-MPR cytoplasmic domain
removing the different clathrin-coated pit localization signals do not
alter the basolateral delivery of the corresponding HCD mutant
(HCD-17,A13A18A45). Even the largest truncation of the CD-MPR tail
did not result in the apical delivery of the truncation mutant
(HCD-
55). Such an HCD mutant cannot trigger the recruitment of AP-1
on Golgi membranes nor can it be endocytosed at the plasma membrane.
Therefore, it appears that this membrane protein devoid of
clathrin-coated pit localization signals is transported to the
basolateral domain of MDCK cells via an AP-1-independent pathway.
Basolateral sorting of membrane proteins generally relies on signals
located in their cytoplasmic domains. We therefore examined the 12 amino acids left in the CD-MPR cytoplasmic tail of HCD-55 for the
presence of putative sorting signals (Fig.
8). First, a YQRL motif is present that
is identical to that found in TGN 38. This determinant was shown to be
important for the retrieval of TGN 38 from the plasma membrane to the
TGN in nonpolarized cells (45, 46) and basolateral sorting in MDCK
cells (47). Hydrophobicity plots of the CD-MPR predict that the
tyrosine residue in this YQRL motif is the last amino acid of the
transmembrane domain (17) and that this motif would be barely
accessible to the sorting machinery. However, it has been noticed that
Golgi-resident proteins have on average shorter transmembrane domains
(
15 residues) than plasma membrane proteins (
20 residues) (48,
49). Because the CD-MPR transmembrane domain is 20-25 amino acids
long, it remained possible that in the context of the Golgi membrane
the tyrosine contained in this YQRL motif could be accessible to the basolateral sorting machinery. A 15-amino acid-long transmembrane domain could provide the minimal spacing for accessibility of this
tyrosine-based motif. For example, the basolateral sorting of the
-amyloid precursor is dependent upon a key tyrosine residue in the
cytoplasmic tail of the protein located only 5 residues away from the
transmembrane domain (50). However, mutation of the tyrosine residue to
an alanine in our most truncated HCD chimera (HCD-
55,A-1) did not
influence its basolateral transport at low levels of expression.
Therefore, this tyrosine-based motif does not appear to be essential
for basolateral transport of the most truncated mutant.
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A basolateral sorting determinant could reside in the RLVV sequence overlapping the YQRL motif (Fig. 8). A similar motif (HRRNV), unrelated to classical coated pit localization signals and closely located near the transmembrane domain, has been shown to mediate basolateral delivery of a truncated version of the poly-Ig receptor (13). Within this sequence motif the histidine, the first arginine, and the carboxyl-terminal valine are most essential for basolateral sorting. Two of three essential residues (arginine and valine) are conserved in the CD-MPR membrane-proximal determinant. Interestingly, the Man-6-P/IGF II receptor cytoplasmic domain also contains a similar sequence motif (RETV) close to the transmembrane segment (Fig. 8). Because further truncations of the 12-residue-long tail of the HCD chimera resulted in retention of the protein in the endoplasmic reticulum (data not shown), we cannot exclude the remote possibility that the transmembrane domain of the CD-MPR contributes to basolateral delivery. A more likely explanation, however, is that these 12 amino acids contain a determinant (possibly the RLVV motif) that mediates basolateral delivery of HCD in the absence of the known clathrin-coated pit localization signals. The final characterization of this signal awaits more extensive mutational analysis. If this sorting determinant mediates the basolateral delivery of the luminal domain of HA used as a reporter, it also remains to be determined to what extent it contributes to CD-MPR trafficking.
It is interesting to note that the overexpression of the most truncated
HCD-55 mutant results in the significant delivery of the protein to
the apical membrane. This observation was not only made with high
expressing MDCK clones (this study) but also with low expressing MDCK
clones treated with butyrate, which induces a 5-10-fold increase in
the expression level (data not shown). HCD-
55 or HCD-
55,A-1 were
significantly missorted to the apical plasma membrane after
butyrate-induced overexpression. Under such conditions, 40% of the
cell surface proteins were present on the apical membrane, strongly
suggesting that a saturable, AP-1-independent transport machinery
recognizes a basolateral sorting determinant present in the 12-amino
acid-long cytoplasmic tail and/or transmembrane domain of CD-MPR. A
similar result was also obtained with the HCD-
17,A13A18A45 mutant.
In contrast, such an increase in the expression level of HCD, which
follows an AP-1-dependent pathway at the exit of the TGN,
had almost no effect on its basolateral delivery. Thus, these two
sorting machineries can be saturated in a different manner. Little is
known about the machineries responsible for basolateral delivery of
membrane proteins in polarized cells. Similarities between several
basolateral and clathrin-coated pit localization signals have suggested
that basolateral transport of membrane proteins requires coat
components related to the assembly proteins of clathrin-coated
vesicles. Thus far, the µ subunits of the AP-1 and AP-2 assembly
proteins have been shown to interact with tyrosine-based motifs in the
yeast two hybrid system (51, 52). In a similar manner, the µ subunit
of the newly described AP-3 complex that shares structural similarities
with AP-1 and AP-2 also recognizes tyrosine-based sorting signals (52).
Several basolateral targeted membrane proteins have been shown to
contain tyrosine-independent sorting determinants. It remains to be
determined whether these tyrosine-independent sorting determinants can
be recognized by coat components related or unrelated to the AP-1 and
AP-2 assembly proteins.
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ACKNOWLEDGEMENTS |
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We thank Dr. J. Skehel for the anti-HA
monoclonal antibody, Dr. E. Ungewickell for the 100/3 anti--adaptin
monoclonal antibody, and Dr. M.-J. Getting for providing the HA
cDNA.
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FOOTNOTES |
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* This work was supported in part by funds from Vaincre les Maladies Lysosomales and the European communities (to B. D.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Present address: Dept. of Biochemistry, Academic Medical Center,
Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.
§ To whom correspondence should be addressed. Present address: Institut de Biologie de Lille, EP CNRS 525, Institut Pasteur de Lille, BP 447, 1 rue Calmette, 59021 Lille Cedex, France. Tel.: 33-3-20-87-10-25; Fax: 33-3-20-87-10-19; E-mail: bernard.hoflack{at}Pasteur-Lille.fr.
1 The abbreviations used are: MDCK, Madin-Darby canine kidney; TGN, trans-Golgi network; MPR, mannose 6-phosphate receptor; CD-MPR, cation-dependent mannose 6-phosphate receptor; HA, hemagglutinin; IGF, insulin-like growth factor; PAGE, polyacrylamide gel electrophoresis; Man-6-P, mannose 6-phosphate; MEM, minimum Eagle's medium; PBS, phosphate-buffered saline; BSA, bovine serum albumin.
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REFERENCES |
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