From the Cancer Research Campaign Nucleic Acid Structure Research Group, Department of Biochemistry, The University, Dundee DD1 4HN, United Kingdom
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ABSTRACT |
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The leu-500 promoter is inactivated
by a mutation in the 10 region but can be activated in topA
Escherichia coli and Salmonella strains. We have
found that the tetA gene plays a vital role in the
topA-dependent activation of a plasmid-borne
leu-500 promoter. In previous studies, the
leu-500 promoter and tetA gene have been arranged divergently. In this study we have reversed the polarity of
the tetA gene, thus locating the leu-500
promoter at the 3
end of tetA. Despite being formally
located in the downstream region of tetA, the
leu-500 promoter is equally well activated in a
topA strain in this environment, even though it is 1.6 kilobase pairs away from the promoter of the reversed tetA
gene. Activation of the leu-500 promoter depends on
transcription and translation of tetA but is largely
insensitive to the function of other transcription units on the
plasmid. These results require a change in viewpoint of the role of
tetA, from local to global supercoiling. We conclude that
transcription of the tetA gene is the main generator of
transcription-induced supercoiling that activates the
leu-500 promoter. Unbalanced relaxation of this
supercoiling leads to a net increase in the negative linking difference
of the plasmid globally, and there is a linear correlation between the
change in global plasmid topology and the activation of the
leu-500 promoter. Thus the leu-500 promoter
appears to respond to the negative supercoiling of the plasmid
overall.
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INTRODUCTION |
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The activation of the leu-500 promoter provides a good
illustration of the possible interrelationships between transcription and the topology of the DNA template in vivo.
leu-500 is a leucine auxotroph of Salmonella
typhimurium (1) that results from an A to G transition in the 10
region of the promoter of the leu biosynthetic operon (2).
It was found that leucine prototrophy was restored in
Salmonella bearing a supX mutation (3). The later
demonstration that supX was identical to topA
(4), the gene encoding DNA topoisomerase I, provided a strong
indication of a functional link between transcription and topology.
Thus the increase in negative supercoiling that should arise from the loss of the supercoiling-relaxation activity from the
Salmonella cell (5) might be expected to assist in the
function of the leu-500 promoter, coupling the additional
free energy of negative supercoiling to the opening of the more
refractory
10 region of the mutant promoter (6, 7).
More recent work in this laboratory has identified an additional level of complexity in this process. The demonstration of a direct requirement for a null topA background (8) led to the suggestion that the leu-500 promoter might be activated by variations in template supercoiling arising from transcriptional-induced supercoiling due to the transcription of a nearby gene (9, 10). According to the twin-supercoiled domain model of Liu and Wang (11), a rotationally hindered RNA polymerase in the elongation phase of transcription will tend to generate positive supercoiling ahead of its passage and negative supercoiling in its wake. These domains will be relaxed by DNA gyrase and topoisomerase I, respectively, in eubacteria, but unbalanced relaxation by topoisomerase activity due to either inhibition or mutation will lead to alteration in the local level of DNA supercoiling (12, 13). Thus the leu-500 promoter might be activated by negative supercoiling arising from the transcription of the putative nearby gene, which would be less efficiently relaxed in topA cells.
Although this model could explain the activation of the chromosomal leu-500 promoter in topA Salmonella, a further complication came to light when we sought to reproduce the effect on a plasmid. We found that we could only obtain significant activity of the leu-500 promoter when the plasmid also bore the gene encoding resistance to tetracycline, tetA. Using such plasmids we could achieve topA-dependent activation of the promoter in either Salmonella (10) or Escherichia coli (14). This implied a key role for the tetA gene, and a number of studies have indicated that the coupled transcription, translation, and membrane insertion of the tetA gene product are essential for efficient oversupercoiling of plasmids in topA eubacterial cells (13, 15-17) due to the anchorage of the transcribing RNA polymerase to the membrane. We showed that activation of the leu-500 promoter on a plasmid did indeed require transcription and translation of the tetA gene and insertion of the TetA polypeptide into the membrane (10, 18).
We can conceive of two roles for the tetA gene in the activation of the leu-500 promoter on a plasmid. First, transcription of the tetA gene could be the primary generator of supercoiling; tethering RNA polymerase to the membrane would be a particularly effective way in which to hinder its rotation about the DNA template, and thus efficient induction of supercoiling might be expected. The second role could be more passive: to provide a barrier to the diffusion of supercoiling. If negative and positive domains of supercoiling were generated by transcription elsewhere on the plasmid, these could diffuse around the circle and cancel each other by rotation about the duplex axis, providing a highly efficient nonenzymatic relaxation mechanism. However a point of anchorage (such as the insertion of the nascent TetA polypeptide into the membrane) should provide a barrier to the diffusion of supercoiling around the plasmid and might thus increase local levels of DNA supercoiling.
In the plasmid pLEU500Tc, with which we first achieved the topA-dependent activation of the leu-500 promoter (10), the tetA gene was oriented divergently to the leu-500 promoter, with a short distance between the promoters. This places the leu-500 promoter immediately upstream of the tetA promoter, which would be consistent with a very local effect whereby the leu-500 promoter responds to a domain of negative supercoiling directly upstream of tetA. We therefore wondered if the leu-500 promoter would still be activated in topA cells if the orientation of the tetA gene were reversed. We find that the leu-500 promoter is activated to the same level under these circumstances and that the activity remains fully dependent on the function of the tetA gene. We conclude that transcription of the tetA gene is the major source of negative supercoiling that activates the leu-500 promoter, but that this is mediated through the global topology of the plasmid.
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MATERIALS AND METHODS |
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Bacterial Strains and Their Growth Conditions
E. coli strains HB101 (F,
hsdS20 (r
B, m
B),
recA13, ara-14, proA2,
lacYI, galK2, rpsL20
(Smr), xyl-5, mtl-1,
supE44,
), and DM800
(
(topA-cysB)204 acrA13 gyrB225) (25, 26) have been used
in the experiments reported here. Bacteria were cultured at 37 °C
with aeration in LB medium or grown on 1.2% LB agar plates. Media were
supplemented with antibiotics as required; ampicillin and kanamycin
were both used at 50 µg/ml and tetracycline was used at 10 µg/ml
(except for strains related to E. coli DM800, where this was
reduced to 2 µg/ml tetracycline). E. coli strains were
transformed with plasmids using the calcium chloride procedure (27).
Plasmid Constructions
The plasmids used in this work are summarized in Table I
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pL500TR-- The plasmid pLEU500Tc (10) was cleaved with NheI and BalI, and pAT153 (28) was digested with EcoRI and BalI. The NheI and EcoRI termini were rendered flush by incubation with 2.5 units of VentR DNA polymerase (NEB) at 72 °C for 30 min. The smaller EcoRI-BalI fragment of pAT153, containing the entire tetA gene, and the larger NheI-BalI fragment of plasmid pLEU500Tc were isolated by preparative gel electrophoresis. The two blunt-ended fragments were then ligated together with T4 DNA ligase, and the resulting plasmid was transformed into E. coli HB101. The plasmid containing the complete tetA gene oriented anticlockwise (see Fig. 1) was identified by restriction digestion of isolated plasmid DNA.
pL500TR.Ptet.rev--
pL500TR was cleaved with
ClaI, and the resulting linear DNA was digested with mung
bean nuclease (35 units/µl for 25 min at 37 °C). The blunt-ended
DNA was religated to generate a plasmid that contained the modified
anticlockwise tetA promoter.
pL500TR.Ptet--
To remove the
10 region of
the original (clockwise) tetA promoter, pL500TR was
partially cleaved with HindIII followed by digestion with
mung bean nuclease (35 units/µl for 25 min at 37 °C). The
blunt-ended DNA was religated and transformed into E. coli HB101. Since the HindIII cleavage could occur at either of
the target sites, restriction digests and DNA sequencing were used to
identify the deletion of clockwise-oriented tetA
promoter.
pL500TR.Ptet
Ptet.rev--
This
plasmid contains deletions of both tet promoters of pL500TR.
It contains a 4-bp1 deletion
at the HindIII site overlapping the clockwise-oriented tetA promoter and a 4-bp deletion at the ClaI
site of the anticlockwise-oriented tetA promoter.
pL500TR.bla--
This plasmid contains a deletion between the
SspI site and the ScaI site of the bla
gene. pL500TR was cleaved at the SspI and ScaI
sites, the largest blunt-ended fragment was isolated by preparative
electrophoresis in an agarose gel, and circularized with T4 DNA
ligase.
pL500TR.bla
Ptet--
This plasmid combines
the deletion between the SspI site and the ScaI
site of the bla gene and the 4-bp deletion at the
HindIII site of the clockwise-oriented tetA
promoter in pL500TR.
Plasmids Containing Translation Terminators within the tetA Gene of pL500TR-- Pairs of complementary oligonucleotides (10) were ligated into the plasmid pL500TR linearized by the appropriate restriction enzyme; NheI (partial digestion was required since there are two NheI sites in pL500TR), BamHI, SalI, and NruI, generating pL500TR.Tet48, pL500TR.Tet96, pL500TR.Tet188, and pL500TR.Tet296, respectively.
pL500TR.Bla12, pL500TR.Bla80-- These plasmids contain translation termination codons inserted into either the Eco57 or ScaI sites within the bla gene of pL500TR. Self-complementary oligonucleotides encoding a universal translation terminator (18) were inserted into the Eco57 or the ScaI sites within the bla gene of pL500TR, generating pL500TR.Bla12 and pL500TR.Bla80, respectively.
Extraction and Analysis of Cellular RNA
RNA was isolated using essentially the method described
previously (10). RNA was prepared from 200-µl cultures in the
mid-exponential growth phase by the addition of an equal volume of 20 mM sodium acetate (pH 5.2), 2% SDS, 0.3 M
sucrose and transferring to a boiling water bath for 1 min. The sample
was then extracted twice with phenol/chloroform, and the nucleic acids
were precipitated with ethanol. After the addition of 0.2 pmol of the
appropriate radioactively [5-32P]-labeled DNA primer,
the sample was heated to 90 °C in 4.5 µl of 50 mM
Tris-HCl (pH 8.0), 50 mM KCl, and rapidly cooled. 25 units
of RNasin (0.5 µl) were added, and the solution was incubated at
43 °C for 20 min before the addition to 12 µl of 70 mM
Tris-HCl (pH 8.0), 70 mM KCl, 15 mM
MgCl2, 15 mM dithiothreitol, 1.3 mM deoxynucleoside triphosphate mixture containing 50 units of moloney murine leukemia virus reverse transcriptase (Superscript Plus; Life
Technologies, Inc.) and incubated at 42 °C for 2 h. cDNA transcripts were electrophoresed in 6% polyacrylamide gels in 90 mM Tris borate (pH 8.3), 10 mM EDTA (TBE)
containing 7 M urea, next to sequence markers generated by
dideoxy sequence reactions (29) using the same primer. After drying the
gels, radioactive fragments were visualized by autoradiography at
70 °C with intensifier screens or with storage phosphor screens
and a 400 S PhosphorImager (Molecular Dynamics). Quantitation of
radioactivity was performed directly upon the phosphorimage using
ImageQuant (Molecular Dynamics).
Analysis of Linking Number of Extracted Plasmid DNA-- E. coli cells were grown in 30 ml of LB plus appropriate antibiotics to mid-exponential growth phase, and the plasmid DNA was extracted using the Wizard Plus DNA extraction system (Promega). The purified DNA was electrophoresed in 1% agarose gels in TBE containing 2 µg/ml chloroquine. After electrophoresis, the gels were subjected to extensive washing in water followed by staining in 1 µg/ml ethidium bromide and further washing in water. The stained gels were photographed under UV illumination with red and green filters to remove background fluorescence. The photographic negatives were scanned electronically, and a negative image was presented.
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RESULTS |
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Reversal of the tetA Gene of pLEU500Tc--
In previous studies we
showed that the activation of the leu-500 promoter on the
plasmid pLEU500Tc in topA S. typhimurium was dependent on
the function of the adjacent tetracycline resistance gene
tetA (10). The orientation of the tetA gene in
pLEU500Tc is opposite to that of the leu-500 promoter,
i.e. the leu-500 promoter is located immediately
upstream of the tetA gene. Thus transcription of
tetA might be the major generator of negative supercoiling
in this local region, by the mechanism of Liu and Wang (11). Activation
of the leu-500 promoter in pLEU500Tc required the coupled
transcription and translation of tetA and the membrane insertion of its product (10, 18). This suggested that membrane insertion of the TetA protein was essential to provide an anchorage point, which might act as a topological barrier against the diffusion of DNA supercoiling. These two related yet distinct roles for the
tetA gene might be dissected if its polarity were reversed in the plasmid, and we therefore constructed a new plasmid pL500TR that
contains a tetA gene oriented anticlockwise in the
conventional depiction of pBR322-based plasmids. The reversed
tetA gene is fully functional, and transformed cells have
normal levels of resistance to tetracycline. pL500TR still contains the
original clockwise tetA promoter, but the gene
(tetA) is truncated at the 48th codon. It also contains the
anticlockwise antitet promoter. The plasmid map of pL500TR
is shown in Fig. 1.
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topA-dependent Activation of the leu-500 Promoter of pL500TR-- In our earlier study, we demonstrated topA-dependent activation of the leu-500 promoter carried on plasmid pLEU500Tc containing a clockwise tetA gene. To investigate the effect of a reversed polarity tetA gene on the activity of the leu-500 promoter, RNA was isolated from pL500TR-carrying topA or top+ E. coli cells in mid-exponential growth, and transcripts initiated at the leu-500 promoter were sought. This was achieved by means of run-off reverse transcription using a primer that hybridizes to the vector sequence upstream of the S. typhimurium DNA (10). A cDNA corresponding to RNA initiated at the leu-500 promoter should be 191 nuceotides in length. Since the antitet promoter (the tetR promoter transcribing the same strand as the leu-500 promoter) is retained on pL500TR, cDNA corresponding to initiation at this promoter would be 281 nucleotides in length and provides a useful reference for quantitation.
The results of the reverse transcription analysis are shown in Fig. 2A. There is a clear band of cDNA corresponding to initiation at the leu-500 promoter in DM800 (
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Activation of the leu-500 Promoter Requires Transcription of the
Reversed tetA Gene--
In its original orientation in pLEU500Tc, the
tetA gene must be transcribed to activate the
leu-500 promoter (10). We therefore investigated whether
this was also required when tetA was reversed in pL500TR.
Plasmid pL500TR.Ptet.rev was constructed containing a
4-bp deletion in the ClaI site upstream of the reversed
tetA promoter (anticlockwise). Cells containing the modified
plasmid are sensitive to tetracycline, demonstrating the inactivation of the tetA gene. Cellular RNA was extracted from DM800
(
topA) harboring pL500TR or
pL500TR.
Ptet.rev and analyzed by reverse transcription
as before. The results (Fig. 3) show that
initiation of transcription at the leu-500 promoter in
pL500TR was significantly reduced by the promoter deletion in the
reversed tetA promoter. Thus the
topA-dependent activation of the
leu-500 promoter in pL500TR requires transcription of the
reversed tetA gene.
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Activation of the leu-500 Promoter Requires Translation of the Reversed tetA Gene-- By analogy with the role of the clockwise tetA gene of pLEU500Tc, it seemed probable that translation would be required in the reversed gene of pL500TR. This was examined by provoking premature termination of translation of the reversed tetA gene by introducing translation terminators at various positions in the coding sequence. This was achieved by introducing complementary oligonucleotides into the NheI, BamHI, SalI, and NruI restriction sites along the tetA gene, thereby generating truncated TetA polypeptides of 48, 96, 188, and 296 amino acids, respectively. These can be compared with the full-length TetA that is 394 amino acids in length. These plasmids are called pL500TR.Tet48, pL500TR.Tet96, pL500TR.Tet188, and pL500TR.Tet296, respectively.
These plasmids were transformed into E. coli DM800 (
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Negative Supercoiling of Reversed-tetA Plasmids Isolated from
topA E. coli--
When plasmids carrying a functioning
tetA gene are isolated from topA E. coli or
S. typhimurium in exponential growth and their linking
number distribution examined by electrophoresis in agarose gels
containing the intercalator chloroquine, it is generally observed that
there is a bimodal distribution of topoisomers, one fraction of which
is very highly negatively supercoiled. We have previously shown this to
be the case for pLEU500Tc and demonstrated a correlation between the
degree of activation of the leu-500 promoter and the extent
of this hypersupercoiled fraction (14). We therefore examined the
plasmids carrying the reversed tetA gene to see if these
were similarly subject to hypersupercoiling.
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Local Gene Expression and the Activation of the leu-500 Promoter in pL500TR-- Analysis of the topA-dependent activation of the leu-500 promoter in pL500TR clearly highlights the importance of the reversed tetA gene. We discussed two conceivable roles for this gene: as a direct generator of negative supercoiling by transcription with hindered rotation of RNA polymerase and as a topological barrier against the diffusion of negative supercoiling. Since the promoter of the tetA gene is a significant distance from the leu-500 promoter in pL500TR, it is possible that the primary role is the latter function and that other more local promoters are important in the generation of supercoiling. We therefore turned our attention to other gene expression occurring within the vicinity of the leu-500 promoter. This arises primarily from the bla gene and the original tetA gene of which the promoter is retained in pL500TR.
To determine the effect of local gene expression on the activity of the leu-500 promoter, a number of new plasmids were constructed. pL500TR.
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Effect of Premature Termination of Translation of the bla Gene on
the Activation of the leu-500 Promoter in pL500TR--
Previous
experiments showed that in the original construct with a clockwise
tetA gene (pLEU500Tc), initiation of transcription at the
leu-500 promoter was influenced by translation of the
bla gene under some circumstances (18). We therefore
examined the effect of modulating the function of the bla
gene on the activation of the leu-500 promoter in the
presence of the reversed tetA gene. Two new plasmids were
constructed to examine the influence of bla translation.
pL500TR.Bla12 and pL500TR.Bla80 contain translation termination codons
inserted into the bla coding sequences at the Eco57 and the ScaI sites, respectively,
generating -lactamase polypeptides shortened from 263 amino acids to
12 or 80 amino acids, respectively.
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DISCUSSION |
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Our results clearly demonstrate that the leu-500 promoter can be activated on a plasmid in topA E. coli by the presence of a tetA gene in either orientation. Activation requires the full function of the tetA gene, but the leu-500 promoter can be located in a position that can be regarded either as primarily upstream or one that is downstream of this gene. Moreover the role of the tetA gene is paramount; although other promoters present in pL500TR are of relatively minor consequence, inactivation of tetA function reduces the activity of the leu-500 promoter to background levels. In summary, the tetA gene is essential for the topA-dependent activation of the leu-500 promoter, but its orientation is unimportant.
It might be regarded as surprising that this effect is independent of tetA orientation; that the activation of the leu-500 promoter is equally efficient when it is placed in what is formally the domain of positive supercoiling (downstream of tetA) (11), as when it is located in the upstream domain of negative supercoiling. We therefore change our perspective from a local view of variation in superhelix density to a more global view. The local view supposes that the leu-500 promoter must be located directly within the domain of negative supercoiling to be activated. In the global view, unbalanced relaxation of transcriptional-induced supercoiling from the tetA gene results in a net reduction in the linking difference of the plasmid. If the tetA gene is the primary generator of supercoiling (because of its membrane anchoring effect), then it will create local domains of negative and positive supercoiling. If only the latter can be relaxed in a topA cell, the overall effect will be to lower the linking number of the plasmid. If the leu-500 promoter is responding to this global change in topology, then it will do so independent of relative orientation or separation.
We arrive at the same conclusion following a second line of argument. As we discussed in the introduction, an alternative role of membrane anchorage by coupled transcription, translation, and insertion of TetA could be to provide a topological barrier so that the domains of positive and negative supercoiling generated by transcription (in theory from any promoter) cannot diffuse around the circular plasmid and undergo self-cancellation by a simple rotation of the helix. If this were true, it would require the existence of a second barrier on the opposite side of the circular plasmid, and it has been suggested that the replication origin might function in this way (19). The combined effect of two such barriers would effectively isolate the lower half of the plasmid in topological terms. However, in pL500TR, the promoter of the reversed tetA gene would be located in this domain, isolated topologically from the leu-500 promoter. Yet we have shown that the single most important factor on the plasmid for the topA-dependent activation of the leu-500 promoter is the tetA promoter. We therefore conclude that it cannot be located in a separate domain and that the barrier model does not hold. We are left with the primary role of membrane anchorage as the provision of rotational hindrance to RNA polymerase transcribing the tetA gene. Since the tetA and leu-500 promoters are separated by more than 1.6 kbp, this must be considered as an essentially global phenomenon in the plasmid.
The global view of the activation is consistent with measurement of the linking difference of isolated plasmids (e.g. Fig. 5), which is a measure of the global topology by definition. This shows that the fraction of hypersupercoiled plasmid DNA is generated whenever the tetA gene is present in cis, whatever its orientation. Indeed, we obtain a linear correlation between the level of activation of the leu-500 promoter in topA E. coli with the fraction of hypersupercoiled plasmid DNA isolated from the cells (Fig. 7). In situ probing of the formation of cruciform structures by alternating adenine-thymine ((AT)n) sequences can be used as a means of testing local negative superhelix density in cellular DNA (20), and we have shown that reporter (AT)n sequences introduced in the region corresponding to that upstream of tetA in pLEU500Tc detect unconstrained oversupercoiling in topA strains (21). However, contrary to initial expectations, we also detected elevated negative supercoiling at (AT)nsequences placed downstream of the tetA gene,2 i.e. in the region that might be expected to experience transcriptional induction of positive supercoiling. Once again this result is more consistent with a global view of the induction of negative plasmid supercoiling in topA cells.
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The topA-dependent activation of the leu-500 promoter in pL500TR does differ in some respects from that in the original pLEU500Tc containing the clockwise tetA gene. One is the effect of bla expression; we observed that bla deletion lowered the level of leu-500 promoter activation in pLEU500Tc (18), whereas there is little influence of bla in the presence of the anticlockwise tetA gene of pL500TR. However, we found that the effect of bla deletion on the leu-500 promoter in pLEU500Tc could be removed when a tac promoter was introduced into this plasmid, suggesting that subtle effects may occur in this region. Another difference is the effect of spacing. When we introduced random DNA fragments between the leu-500 and tetA promoters of pLEU500Tc, this reduced the level of initiation of transcription at the former, whereas in pL500TR, the crucial Ptet.rev is almost diametrically opposite to the leu-500 promoter. At present we are unable to account for this difference.
There have been reports of activation of the leu-500 promoter in topA cells using plasmids that do not include the tetA gene (22, 23). We find these observations perplexing, because in our experiments the role of the tetA gene is paramount. It is conceivable that other factors play a role in these constructs, but it is possible that the overall level of activation of transcriptional initiation was lower in those investigations. It is beyond question that in the plasmids based upon pLEU500Tc, the role of the tetA gene is essential for the observed level of activation and cannot be replaced by any other gene that we have explored. Moreover, correlation with the physical level of hypersupercoiling in our plasmids has been independently confirmed by the experiments of Mojica and Higgins (24), who measured the level of unconstrained plasmid supercoiling using an intercalation assay.
In summary, the leu-500 promoter is activated highly efficiently in topA cells when it is borne on a plasmid carrying the tetA gene in cis, irrespective of orientation. The most probable explanation is that it is activated by negative supercoiling arising from the transcription of the tetA gene and that this process is most effective when RNA polymerase is effectively tethered due to coordinate transcription, translation, and membrane insertion. The coupling between the promoters can be fully explained by topological effects operating within the plasmid globally.
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ACKNOWLEDGEMENTS |
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We thank Dr. Richard Bowater for discussions and the Medical Research Council and Cancer Research Campaign for financial support.
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FOOTNOTES |
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* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
A European Molecular Biology Organization fellow.
§ To whom correspondence should be addressed. Tel.: 44-1382-344243; Fax: 44-1382-201063; E-mail: dmjlilley{at}bad.dundee.ac.uk.
1 The abbreviation used is: bp, base pair(s).
2 R. P. Bowater, D. Chen, and D. M. J. Lilley, unpublished data.
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REFERENCES |
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