From the Department of Molecular Biology, Max-Planck Institut für Biochemie, Am Klopferspitz 18a, 82152 Martinsried, Germany
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ABSTRACT |
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Kinesins comprise a large family of microtubule-based motor proteins, of which individual members mediate specific types of motile processes. Using the ezrin domain of the protein-tyrosine phosphatase PTPD1 as a bait in a yeast two-hybrid screen, we identified a new kinesin-like protein, KIF1C. KIF1C represents a member of the Unc104 subfamily of kinesin-like proteins that are involved in the transport of mitochondria or synaptic vesicles in axons. Like its homologues, the 1103-amino acid protein KIF1C consists of an amino-terminal motor domain followed by a U104 domain and probably binds to target membranes through carboxyl-terminal sequences. Interestingly, KIF1C was tyrosine-phosphorylated after peroxovanadate stimulation when overexpressed in 293 or NIH3T3 fibroblasts or in native C2C12 cells. Using immunofluorescence, we found that KIF1C is localized primarily at the Golgi apparatus. In brefeldin A-treated cells, the Golgi membranes and KIF1C redistributed to the endoplasmic reticulum (ER). This brefeldin A-induced flow of Golgi membranes into the ER was inhibited in cells transiently overexpressing catalytically inactive KIF1C. In conclusion, our data suggest an involvement of tyrosine phosphorylation in the regulation of the Golgi to ER membrane flow and describe a new kinesin-like motor protein responsible for this transport.
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INTRODUCTION |
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Proteins destined for secretion, plasma membrane, or intracellular organelles are synthesized in the endoplasmic reticulum (ER)1 and transported along the secretory pathway through the Golgi complex by coated vesicular carriers. Adjacent to the stacked cisternae of the Golgi complex, regions of the ER membrane bud off to form vesicles. These vesicles then seem to fuse with a tubular network at the cis face of the Golgi apparatus. Vesicles are formed from the ER by the action of the coat protein II complex, and they incorporate vesicle targeting molecules (known as v-SNAREs). These proteins interact with corresponding t-SNAREs, which are present on the target membranes of the cis-Golgi network. Proteins not only leave the ER near Golgi membranes but emerge at sites that are distributed apparently randomly over the cell. These sites are marked by little groups of vesicles and tubules (vesicular-tubular clusters). The vesicular-tubular clusters, containing the secreted proteins, travel along microtubules (MTs) to the Golgi apparatus, where they fuse with the cis-Golgi network. This flow of membranes into the Golgi complex needs to be counterbalanced by a reverse (Golgi to ER) membrane transport pathway that involves coat protein I-coated vesicles traveling along MTs (for reviews, see Refs. 1 and 2)
Intracellular transport along microtubules is controlled by two microtubule-based mechanochemical motor protein superfamilies: the dyneins and the kinesin-like proteins (KLPs) or kinesin family proteins (KIFs). Nucleation of microtubule growth by the microtubule-organizing center, which in many cell types is located close to the nucleus, results in a radially polarized array of MT with their fast growing (plus) ends at the periphery of the cell and their slow growing (minus) ends at the cell center. Dyneins and some members of the kinesin superfamily drive movement toward MT minus ends, whereas conventional kinesin and most kinesin-related proteins are involved in the movement of membrane-bound organelles and vesicles toward the plus ends of microtubules. In addition to their function in vesicle transport, motor proteins of both superfamilies are also involved in mitosis and meiosis. Recently, dynein was identified by Presley et al. (3) as the motor protein mediating the vesicle transport from the ER to the Golgi complex.
Kinesins share a conserved motor domain of ~340 amino acids, and
similarities have been used to construct molecular phylogenies of the
KLPs with so far eight subfamilies and numerous outgroups being
described (4, 5). Outside of the motor domain, the amino acid sequences
of the KLPs differ from each other, reflecting their different
functions. Like kinesin, most KLPs have a central -helical region.
This region is judged to mediate the homodimerization of kinesin heavy
chains (KHCs). The tail of kinesin is associated with two kinesin light
chains and thought to bind the cargo and/or accessory proteins. An
integral membrane protein, kinectin, has been identified to act as the
link between kinesin and its cargo (6). Very few associating proteins
have been identified for KLPs. Candidates include KAP3 for KIF3A/3B
regulating membrane binding of the KIF3 complex (7) and the
serine/threonine phosphatase PP1 for KLP38B (8). With only little
knowledge about associated proteins, the regulation of motility and
cargo binding of KLPs is poorly understood. Several reports have
suggested that serine phosphorylation, which was observed in kinesin
and kinectin (9, 10), may play an important role. By contrast, no
function of tyrosine phosphorylation has been demonstrated, although it
has been reported for KHC (11).
Here we describe the cloning and characterization of a new kinesin-like motor protein, KIF1C, which is a ubiquitously expressed member of the U104 subfamily. KIF1C is tightly associated to the Golgi membranes and required for the retrograde transport of Golgi vesicles to the ER. We also provide evidence that this motor protein is tyrosine-phosphorylated in intact cells, which adds tyrosine phosphorylation as a putative regulatory element in the vesicle transport between Golgi and ER.
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EXPERIMENTAL PROCEDURES |
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Yeast Two-hybrid Screen--
The ezrin-like domain of PTPD1
(Ref. 12; amino acids 1-345) was cloned into the LexA fusion protein
vector pBTM116 (kindly provided by S. Hollenberg and J. Cooper) and
transformed into Saccharomyces cerevisiae strain L40
(MATa trp1 leu2 his3 LYS2::lexA-HIS3 URA3::lexA-lacZ; Ref. 13), generating the L40
lexA-D1ezrin strain. A skeletal muscle cDNA library
fused to the GAL4 activation domain in the pGAD10 vector
(CLONTECH) was transformed into the L40
lexA-D1ezrin strain, and 106 transformants were
screened for interaction as described (14). Yeast plasmid DNA was
isolated from His+ -Gal+ colonies, rescued
into Escherichia coli HB101, retransformed into L40
lexA-D1ezrin, and assayed for
-galactosidase activity and growth on dropout medium with complete supplement lacking Trp, Leu,
Ura, Lys, and His (Bio101). The specificity of the interaction between
the ezrin domain of PTPD1 and potential candidates was proven by
transforming the candidate plasmid also into the L40 lexA and the L40
lexA-laminin strain.2
DNA Sequence Analysis and cDNA Library Screening--
Clones
interacting specifically with PTPD1 in the two-hybrid screen were
subjected to sequencing by the dideoxynucleotide chain termination
method. Nonredundant nucleotide data bases (GenBankTM and
EMBL) were searched for homologous sequences using the FASTA search
algorithm. To obtain 5'-terminal KIF1C sequences, the partial KIF1C
cDNA obtained from the two-hybrid screen (clone K1, base pairs
1196-4082) was used to screen a human hippocampus gt11 cDNA
library (CLONTECH) by standard protocols (15).
cDNA sequences encoding the amino-terminal part of KIF1C were fused
by conventional DNA cloning techniques to the KIF1C clone from the
two-hybrid screen generating a full-length cDNA of KIF1C.
Northern (RNA) Blot Analysis-- A multiple tissue Northern blot was obtained from CLONTECH. Hybridization was performed at 68 °C with 5 × 106 cpm/ml of 32P-random primed DNA probe as described by the manufacturer.
Antisera--
The KIF1C rabbit polyclonal antiserum was raised
against a glutathione S-transferase fusion protein of KIF1C
(amino acids 362-725). The PTPD1 and the PTPH1 antisera were raised
against peptides (PTPD1, residues 618-631; PTPH1, residues 900-913)
coupled to keyhole limpet hemocyanine. The anti-phosphotyrosine
antibody 4G10 was obtained from Upstate Biotechnology, Inc., and the
58K and -tubulin antibodies were obtained from Sigma.
Construction of Expression Plasmids--
For transient
expression in 293 and NIH3T3 cells, all cDNAs were cloned by
standard procedures into the cytomegalovirus immediate early
promotor-based expression plasmid pRK5 (16). For generation of the
catalytically inactive mutant of KIF1C, KIF1C-K103A, the codon of
lysine 103 was mutated to a codon for alanine using the method of
Kunkel et al. (17). In the NT-KIF1C protein amino acids
1-571 are replaced by the sequence MYPYDVPDYA, which contains the
nonapeptide of the hemagglutinin protein of human influenza virus.
Cell Lines and Cell Culture-- NIH3T3, RD, and C2C12 cells were grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum (Life Technologies, Inc.) and 2 mM glutamine. 293 and BOSC23 cells were maintained in F-12/Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum and 2 mM glutamine. PC12 cells were grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum, 10% horse serum, and 2 mM glutamine. Before stimulation with peroxovanadate (POV) (18), the cells were serum-starved for 20 h. Taxol (Sigma) was used at a concentration of 10 µM, colcemid (Sigma) at a concentration of 0.1 µg/ml, and brefeldin A (BFA; Sigma) at a concentration of 5 µg/ml. KIF1C stably overexpressing NIH3T3 cells (3T3/X/KIF1C) were generated by retroviral infection of exponentially growing cells. The retrovirus was obtained by transfection of BOSC23 cells with the pLXSN-KIF1C plasmid (19).
Transient Expression, Cell Lysis, and
Immunoprecipitation--
Transfection of 293 and NIH3T3 cells was
performed using the method of Chen and Okayama (20). Treatment and
lysis of the cells were performed as described (16). Briefly, the cells
were lysed in 1 ml of lysis buffer/10-cm plate (1% Triton X-100, 50 mM HEPES, pH 7.5, 10% glycerol, 150 mM NaCl,
1.5 mM MgCl2, 1 mM EGTA, 10 mM sodium pyrophosphate, 100 mM NaF, 1 mM sodium orthovanadate, 10 µg/ml aprotinin, 1 mM phenylmethylsulfonyl fluoride), and the lysates were
precleared by centrifugation at 13,000 rpm for 15 min at 4 °C. The
lysates were adjusted for equal protein concentration, and the
appropriate antibody (2 µl of serum or 2 µg of antibody) and
protein A-Sepharose (Amersham Pharmacia Biotech) were added and
incubated for at least 3 h at 4 °C. The immunoprecipitates were
washed extensively with HNTG buffer (20 mM HEPES, pH 7.5, 150 mM NaCl, 0.1% Triton X-100, 10% glycerol, 10 mM NaF, 1 mM sodium orthovanadate), separated
on an 8% SDS-polyacrylamide gel, transferred to a nitrocellulose
membrane, and incubated with the respective antibody. Bound antibodies
were visualized using the ECL system (Amersham Pharmacia Biotech) in
conjunction with horseradish peroxidase-conjugated goat-anti-mouse or
goat-anti-rabbit antibodies (Bio-Rad, Sigma). Before reprobing, blots
were incubated for 30 min in 62.5 mM Tris-HCl pH 6.8, 2%
SDS, and 0.1% -mercaptoethanol at 50 °C.
Immunofluorescence--
For immunofluorescence studies, NIH3T3
cells grown on uncoated glass coverslips were fixed for 20 min in
ice-cold methanol at 20 °C, washed with phosphate-buffered saline,
and incubated for 15 min with 0.1% NaBH4 and 0.1 M glycine in phosphate-buffered saline to block
autofluorescence. Nonspecific antibody binding was blocked for 45 min
with PBG (phosphate-buffered saline with 0.045% fish gelatin)
containing 5% normal goat serum and 1% bovine serum albumin.
Incubation with the primary antibody was done for 1.5 h at
37 °C after dilution in PBG containing 5% normal goat serum; the
affinity-purified KIF1C antibody was used at a concentration of 20 ng/µl unless otherwise stated, and the 58K antibody (Sigma) was
diluted as recommended by the manufacturer. After five washes with PBG,
primary antibody binding was detected with isotype-specific secondary
antibody conjugated with either DTAF or Cy3 (Dianova). The coverslips
were mounted in PermaFlour (Immunotech). Triton extraction of cells for
immunofluorescence under MT-stabilizing conditions was performed prior
to fixation for 5 min on ice in a buffer containing 100 mM
Pipes, pH 7.0, 5 mM MgSO4, 10 mM
EGTA, 2 mM dithiothreitol, 10% glycerol, 0.75% Triton
X-100, 10 µM taxol (Sigma), 10 µg/ml aprotinin, 1 mM phenylmethylsulfonyl fluoride. Lysis under
MT-destabilizing conditions was performed in the lysis buffer described
above lacking EGTA and with the addition of 80 µM
CaCl2 (21).
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RESULTS |
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Cloning of KIF1C-- PTPD1 belongs, together with PTPPEZ (22), PTPH1 (23), PTPMEG (24), and PTPBAS (25), to the group of cytosolic protein-tyrosine phosphatases containing an ezrin-like domain. This domain is homologous to the amino-terminal portion of the cytoskeleton-associated proteins of the band 4.1 superfamily, and in the case of the ERM proteins (ezrin, radixin, and moesin) mediates the association with the membrane-spanning protein CD44 (26), thereby linking the cytoskeleton with the plasma membrane. By contrast, the functional relevance of this ezrin-like domain in PTPs has not yet been described. To identify proteins binding to the ezrin-like domain of PTPD1, we employed the yeast two-hybrid system (for a review, see Ref. 27). A human skeletal muscle cDNA library was screened using the ezrin-like domain of PTPD1 (amino acids 1-345) fused to the lexA DNA binding domain as the bait. Out of 106 clones screened, nine clones were found to interact with the ezrin-like domain of PTPD1 in yeast. Sequence analysis suggested that the clones encoded cDNAs derived from the same gene. None of the clones isolated contained a full-length open reading frame, but all of them encoded an overlapping sequence of 96 amino acids (amino acids 714-809), apparently sufficient for binding PTPD1. The missing 5'-end of the cDNA was isolated from a human hippocampus library by conventional screening using the K1 cDNA obtained in the two-hybrid screen (base pairs 1196-4082) as a probe. The sequence showed greatest homology to the kinesin-like motor protein KIF1B (28), and in accordance with the nomenclature introduced by Nangaku et al. (28) and Okada et al. (29), this protein was named KIF1C. The complete cDNA of KIF1C has a size of 4.1 kilobase pairs and contains a single open reading frame coding for a 1103-amino acid protein with a calculated molecular weight of 122,000 (Fig. 1). KIF1C belongs to the family of kinesin-like proteins as evidenced by the presence of the kinesin signature (amino acids 242-253) in the amino-terminal motor domain. In addition, the protein contains a P-loop sequence (amino acids 97-104) characteristic for ATP- or GTP-binding proteins (30) and the consensus sequence of the nucleotide binding motif for kinesins (31, 32). Like all MT-dependent motor proteins, KIF1C is capable of binding microtubules in vitro and in intact cells (data not shown). Close to the amino-terminal motor domain, KIF1C contains a U104 domain, which is also found in the other kinesins belonging to the Unc104 subfamily and in the AF-6 and Cno gene products (33). The function of the U104 domain has not yet been defined.
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Expression of KIF1C-- To study the size of the transcript and the tissue distribution of KIF1C, Northern blot analysis using the cDNA fragment K9 (base pairs 2252-3126) as a probe was performed. A KIF1C transcript of approximately 4.2 kilobase was detected in all tissues examined but with especially high expression in heart and skeletal muscle (Fig. 3A).
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Binding of KIF1C to PTPD1-- As described above, KIF1C was isolated as a cDNA encoding a protein capable of binding to the ezrin-like domain of PTPD1. Nine cDNAs were isolated, which all differ in length from each other and thus are independent and not derived from clonal amplification of the same original cDNA. They all contain a region in the carboxyl-terminal half of KIF1C, encoding the amino acids 714-809. This demonstrates that these 96 residues are sufficient to bind PTPD1 (Fig. 4A). While the two-hybrid system has now been successfully used many times to demonstrate protein-protein binding, it is also known to be capable of producing false positives. To confirm the interaction of PTPD1 and KIF1C in a different system, we examined the association under the condition of overexpression within cells. Therefore, we expressed KIF1C alone or together with PTPD1 transiently in 293 cells. After immunoprecipitations using the PTPD1 antiserum, the precipitated proteins were separated by SDS-PAGE, transferred to nitrocellulose, and blotted with the affinity-purified KIF1C antibody. As shown in Fig. 4B, KIF1C coprecipitated with PTPD1. The specificity of the KIF1C interaction was investigated in a similar coexpression experiment with a related tyrosine phosphatase, PTPH1, also containing an amino-terminal ezrin-like domain. However, no coprecipitation of KIF1C with PTPH1 could be observed, although KIF1C was expressed (Fig. 4B). Furthermore, we tested the ezrin-like domains of PTPBAS, PTPMEG, and PTPD2 and did not find an association with KIF1C (data not shown). The binding of KIF1C is therefore specific for PTPD1, and future work is necessary to show the physiological relevance of this interaction.
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Tyrosine Phosphorylation of KIF1C-- Since KIF1C was identified by its association with a PTP, we investigated whether KIF1C itself can be tyrosine-phosphorylated. We therefore expressed KIF1C in 293 cells and stimulated the cells with POV. In a parallel experiment, we coexpressed KIF1C with the cytosolic tyrosine kinase c-Src. As shown in Fig. 5A, KIF1C was tyrosine-phosphorylated upon treatment with POV and also upon coexpression with c-Src. To prove equal amounts of KIF1C expression in the experiment, we reprobed the filter with KIF1C antibody. The lower band probably represents an unphosphorylated degradation product.
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Localization of KIF1C in NIH3T3 Cells-- To elucidate the functions of KIF1C, we performed immunofluorescence experiments utilizing the affinity-purified antibody against endogenous KIF1C. Studies in NIH3T3 fibroblasts revealed that KIF1C is localized mainly on a tubular structure close to the nucleus, which is reminiscent of the stacks of the Golgi apparatus (Fig. 6A). In a control experiment, the Golgi apparatus was detected using a monoclonal antibody against the protein 58K (Fig. 6C) which is described to be located specifically at the cis/medial site of the Golgi apparatus by binding simultaneously to microtubules and the Golgi membranes (39, 40). To prove that KIF1C indeed colocalizes with the Golgi, we performed double staining of KIF1C and 58K. Conventional microscopy (Fig. 6, D and E) as well as laser confocal microscopy (data not shown) clearly demonstrated the colocalization of KIF1C with the 58K protein at the Golgi stacks. Treatment of cells with taxol and the microtubule-disrupting agent colcemid leads to a fragmentation of the Golgi membrane system into numerous vesicle-like structures of varying size that are distributed throughout the cytoplasm (41, 42). This characteristic scattered pattern was also found for KIF1C after incubation of the cells for 4 h either with taxol or with colcemid (data not shown).
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Inhibition of the Flow of Golgi Membranes into the ER by Transient Overexpression of Catalytically Inactive KIF1C-- Golgi to ER transport is MT-dependent and occurs toward the plus ends of microtubules (40). Since all proteins of the Unc104 subfamily are described to be MT plus end-directed motors (28, 29), we wondered whether KIF1C functions as a vesicle-transporting motor protein in the membrane flow from the Golgi to the ER. A lysine to alanine mutation in the P-loop has been described for other ATP-binding proteins to abolish ATP binding (30, 46). KIF1C-K103A carries such a mutation and is catalytically inactive, while its other properties should not be affected. Consequently, overexpressed KIF1C-K103A should compete with and replace endogenous KIF1C on Golgi membranes. We overexpressed KIF1C-K103A transiently in NIH3T3 cells and analyzed these cells by immunofluorescence. A double staining of the cells using the 58K antibody and the affinity-purified KIF1C antibody was performed. The concentration of the KIF1C antiserum was chosen to detect KIF1C only in overexpressing cells. As shown in Fig. 7A, overexpressed KIF1C-K103A primarily localized to the Golgi compartment but was also found diffusely distributed in the cytoplasm due to the high expression level. The morphology of the Golgi apparatus as evidenced by the localization of the marker protein 58K was not significantly altered in these cells (Fig. 7B). If KIF1C is the proposed motor protein involved in the Golgi to ER vesicle transport, the BFA-induced flow of the Golgi vesicles to the ER should be inhibited in cells overexpressing the inactive KIF1C-K103A. In Fig. 7, C and D, the morphology of the Golgi is shown after 15 min of incubation of the cells with BFA. While in KIF1C-K103A-overexpressing cells the Golgi still formed discrete structures and overexpressed KIF1C-K103A primarily localized to the Golgi apparatus, in untransfected cells the 58K protein was redistributed to the ER and no juxtanuclear Golgi structure could be detected.
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DISCUSSION |
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In the present study, we used the ezrin-like domain of PTPD1 in a functional screen to detect possible targets of the phosphatase, and we identified KIF1C, a protein belonging to the family of kinesin-like proteins. The association observed in the yeast two-hybrid screen was verified in vitro and in 293 cells transiently overexpressing both proteins. Although the evaluation of the physiological relevance of this association is still in progress, this association of a kinesin-like motor protein with a PTP is an important finding and may lead to new insights into the regulation of intracellular membrane flow by tyrosine phosphorylation.
A number of kinesin-like proteins have recently been identified by a
variety of methods, including a systematic polymerase chain reaction
screen of a murine brain cDNA library (47). In this screen the
mammalian members of the Unc104 subfamily, namely KIF1A (29) and KIF1B
(28), were cloned and further characterized. Sequence analysis of KIF1C
revealed a high similarity with KIF1B in the motor domain but weaker
homology in the central or tail domains. We therefore consider KIF1C to
be a new member of the Unc104 subfamily with similar properties as
described for KIF1B, which is a plus end-directed
microtubule-dependent motor protein. Although we have not
yet demonstrated the motor activity of KIF1C itself, there are several
hints suggesting such an activity; next to the high homology between
KIF1C and KIF1B in the motor domain and the presence of the conserved
kinesin nucleotide binding motifs (32), immunoprecipitations of
-tubulin from lysates of 293 cells resulted in a coprecipitation of
KIF1C. In addition, KIF1C associated with purified MT (data not shown).
After lysis of NIH3T3 cells using MT-stabilizing conditions prior to
fixation, we could still detect KIF1C in immunofluorescence
experiments, whereas it was depleted after lysis of the cells under
MT-destabilizing conditions, a behavior that has also been observed for
the motor protein dynein (21). Finally, transient overexpression of
wild-type KIF1C in NIH3T3 fibroblasts resulted in an accumulation of
KIF1C in the cell periphery. This is similar to experiments using
neurons, where accumulation of motor proteins on the proximal or distal site of ligated axons indicates plus or minus end-directed movement (48). It supports the idea that KIF1C indeed is a plus end-directed motor protein that moves under the conditions of overexpression along
MTs to the cell periphery. In vitro data suggested that the
variable tail domain of KHC is responsible for vesicle cargo binding
(49). Since the amino-terminally truncated
NT-KIF1C protein, lacking
the motor and the U104 domain, is still capable of binding Golgi
membranes, our data indicate that for KIF1C the vesicle binding site is
also located in the carboxyl terminus of the protein. We thus assigned
the two characteristic features of motor proteins, which are motor
activity and cargo binding, to the amino terminus and the carboxyl
terminus, respectively.
In contrast to KIF1A, KIF1B and KIF1C are expressed in a wide variety of tissues including brain, skeletal muscle, heart, and intestine, suggesting a general function in intracellular transport. This assumption has been proven for KIF1B, which was found to transport mitochondria along microtubules (28), whereas KIF1A, being exclusively expressed in brain, is involved in the transport of synaptic vesicles in axons (29). We have shown in our immunofluorescence studies that KIF1C associates to the membranes of the Golgi apparatus, as evidenced by colocalization with the Golgi marker protein 58K.
The mechanism of vesicle transport from the Golgi to the ER has been a subject of controversial experiments. Based on microinjection of anti-KHC antibody into fibroblasts, it was suggested that this transport is mediated by kinesin (50). However, by using antisense oligonucleotide techniques, Feiguin et al. (51) have demonstrated that kinesin is responsible for maintaining the Golgi structure in astrocytes but not for the BFA-induced Golgi to ER transport. By expression of a dominant negative KHC mutant in fibroblasts, Nakata and Hirokawa (52) demonstrated that KHC is involved in the anterograde transport of lysosomes but not in the retrograde Golgi to ER transport. The different techniques that have been used could account for these in part contradictory results. The microinjected antibody against KHC may cross-react with other KLPs due to the high homology between the kinesin superfamily members. It is therefore possible that other proteins of the kinesin superfamily are involved in the Golgi to ER pathway that are not affected by the antisense approach and the dominant negative mutant but are blocked by the microinjected antibody (53). We used a similar technical approach as Nakata and Hirokawa (52) by transiently expressing a catalytically inactive form of the protein of interest. Consequently, our data support their result that KHC is not involved in the Golgi to ER membrane flow and suggest a newly identified motor protein, KIF1C, for this process.
The predicted cycling of KIF1C between Golgi and ER must be tightly regulated to prevent an imbalance between the two compartments. One possible regulatory element could be the observed tyrosine phosphorylation of KIF1C. Based on in vitro experiments, a basal tyrosine phosphorylation of KHC has already been suggested by Matthies et al. (11), but the physiological role of this phosphorylation remained unclear. By contrast, an extensive in vivo phosphorylation on serine has been described for kinesin and kinesin-like proteins as well as for dynein by several groups (9, 54-59). Serine phosphorylation of kinesin by protein kinase A and protein kinase C had no effect on the velocity of microtubule translocation of kinesin in vitro (54) but remarkably reduced the affinity of kinesin to vesicles (55). We found that a part of the cellular pool of KIF1C was tyrosine-phosphorylated even in serum-starved cells. This tyrosine phosphorylation was enhanced by treatment of the cells with the PTP inhibitor POV. Since tyrosine phosphorylation and dephosphorylation are regulative elements in many cellular processes (60), we speculate that the observed tyrosine phosphorylation of KIF1C could be an important regulator of either its motor activity or its association with distinct vesicles and thereby its localization to different compartments within the cell.
In conclusion, KIF1C is a ubiquitously expressed motor protein that belongs to the Unc104 subfamily of KLPs. In the cell it mainly localizes to the Golgi apparatus and probably is involved in the membrane trafficking from the Golgi to the ER. Future work is necessary to determine the role of the observed tyrosine phosphorylation and the physiological relevance of the association of KIF1C with the PTPD1.
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ACKNOWLEDGEMENTS |
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We thank S. Hollenberg and J. Cooper for providing two-hybrid reagents, N. Tonks for providing the PTPH1 cDNA, and I. Sures for critically reading the manuscript.
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FOOTNOTES |
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* This work was supported by a grant from Sugen Inc.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) U91329 (KIF1C cDNA).
Present address: Medical Clinic IV, University of Tübingen,
Otfried-Müller-Str. 10, 72076 Tübingen, Germany.
§ Present address: Max-Planck Institut für Entwicklungsbiologie, Spemannstr. 35, 72076 Tübingen, Germany.
¶ Present address: Novo-Nordisk, Novo Allee, 2880 Bagsværd, Denmark.
To whom correspondence should be addressed. Tel.:
49-7071-2987599; Fax: 49-7071-295974; E-mail:
rrlammer{at}med.uni-tuebingen.de.
The abbreviations used are: ER, endoplasmic reticulum; BFA, brefeldin A; KHC, kinesin heavy chain; KIF, kinesin family protein; KLP, kinesin-like protein; MT, microtubule; POV, peroxovanadate; PTP, protein-tyrosine phosphatase; PAGE, polyacrylamide gel electrophoresis.
2 S. Hollenberg and J. Cooper, unpublished results.
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REFERENCES |
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