Basolateral Sorting of the Cation-dependent Mannose 6-Phosphate Receptor in Madin-Darby Canine Kidney Cells
IDENTIFICATION OF A BASOLATERAL DETERMINANT UNRELATED TO CLATHRIN-COATED PIT LOCALIZATION SIGNALS*

Ben DistelDagger , Ulrike Bauer, Roland Le Borgne, and Bernard Hoflack§

From the European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69012 Heidelberg, Federal Republic of Germany

    ABSTRACT
Top
Abstract
Introduction
Procedures
Results
Discussion
References

In polarized Madin-Darby canine kidney (MDCK) cells, sorting of membrane proteins in the trans-Golgi network for basolateral delivery depends on the presence of cytoplasmic determinants that are related or unrelated to clathrin-coated pit localization signals. Whether these signals mediate basolateral protein sorting through common or distinct pathways is unknown. The cytoplasmic domain of the cation-dependent mannose 6-phosphate receptor (CD-MPR) contains clathrin-coated pit localization signals that are necessary for endocytosis and lysosomal enzyme targeting. In this study, we have addressed the function of these signals in polarized sorting of the CD-MPR. A chimeric protein, made of the luminal domain of the influenza virus hemagglutinin fused to the transmembrane and cytoplasmic domains of the CD-MPR was stably expressed in MDCK cells. This chimera (HCD) is able to interact with the AP-1 Golgi-specific assembly proteins and is detected on the basolateral plasma membrane of MDCK cells where it is endocytosed. Deletion analysis and site-directed mutagenesis of the cytoplasmic domain of the CD-MPR indicate that HCD chimeras devoid of clathrin-coated pit localization signals are still transported to the basolateral membrane where they accumulate. A HCD chimera containing only the transmembrane domain and the 12 membrane-proximal amino acids of the CD-MPR cytoplasmic tail is also found on the basolateral membrane but is unable to interact with the AP-1 assembly proteins. However, the overexpression of this mutant results in partial apical delivery. It is concluded, therefore, that the basolateral transport of this chimera requires a saturable sorting machinery distinct from AP-1.

    INTRODUCTION
Top
Abstract
Introduction
Procedures
Results
Discussion
References

The plasma membrane of polarized cells can be divided into two distinct domains, apical and basolateral, which exhibit different protein and lipid compositions. The generation and the maintenance of these domains require a continuous supply of newly synthesized components. In MDCK1 cells, newly synthesized membrane proteins destined for the basolateral or the apical surface are sorted in the trans-Golgi network (TGN) and packaged into distinct transport vesicles (1). Vesicular transport from the TGN to the apical or basolateral plasma membrane domains are mechanistically different. Although the docking/fusion of transport vesicles with the basolateral plasma membrane relies, like many transport steps, on the presence of the common fusion machinery involving NSF/SNAP proteins, the apical delivery appears to be independent of these proteins (2). More recent studies have indicated that nonpolarized cells also make use of two types of transport intermediates for the delivery of membrane proteins to the plasma membrane, one dependent on the presence of NSF/SNAP proteins and another independent of these complexes (3). Thus, polarized and nonpolarized cells have fairly similar overall organization of membrane traffic within the secretory pathway.

Thus far, two distinct features have been shown to determine sorting to the apical domain: first, the glycosylphosphatidylinositol anchor of membrane proteins (4, 5) and second, the mannose-rich core part of N-glycans present in the luminal domain of proteins (6). Many studies have now illustrated that sorting of membrane proteins to the basolateral plasma membrane is determined by the presence of specific, dominant protein determinants in their cytoplasmic domains (reviewed in Ref. 7). Extensive mutagenesis has uncovered two types of sorting motifs for basolateral delivery. First, there are those related to signals for clathrin-coated pit localization, which either rely on a key tyrosine residue, like those found in the LDL receptor (proximal determinant) (8), the vesicular stomatatis virus G protein (9), and lysosomal membrane glycoproteins (10), or on a di-leucine motif, like in the IgG Fc receptor (11). Second, there are basolateral targeting signals that are unrelated to determinants for clathrin-coated pit localization. Examples can be found in the LDL receptor (distal determinant) and in the poly-IgA receptor (8, 12, 13). Interestingly, the same (or a very closely related) basolateral sorting signal can mediate the recycling of endocytosed membrane proteins from endosomes back to the plasma membrane (14, 15). The similarities between the determinants responsible for endocytosis, basolateral sorting, and plasma membrane recycling suggest that these processes are extremely related and involve similar sorting machineries that remain to be characterized.

In addition to sorting membrane proteins destined for the apical or basolateral domains in the TGN, the polarized MDCK cells must also sort their newly synthesized lysosomal hydrolases bound to the mannose 6-phosphate receptors (MPRs). Previous studies have shown that one of the two MPRs, the mannose 6-phosphate/insulin-like growth factor II receptor (Man-6-P/IGF II), traffics within the basolateral domain of MDCK cells because it can be detected on the basolateral membrane of these cells (16). In nonpolarized cells, the endocytosis of this receptor relies on a tyrosine-based motif, whereas that of the other MPR, the cation-dependent mannose 6-phosphate receptor (CD-MPR), requires a weak tyrosine-based motif and a dominant motif containing two phenylalanine residues (17). On the other hand, efficient lysosomal enzyme targeting requires the presence of a di-leucine-based motif present at the carboxyl terminus of both MPRs (18-20). In addition, the signals required for efficient endocytosis of the MPRs contribute, although weakly, to efficient lysosomal enzyme targeting (19). The MPRs and their bound lysosomal enzymes are known to be sorted in the TGN via clathrin-coated vesicles. The first step in the formation of these transport intermediates is the interaction of the AP-1 Golgi-specific assembly proteins with TGN membranes. The MPRs are part of the membrane components that permit the efficient recruitment of AP-1 on membranes (21-23), a process regulated by the small GTPase ARF-1 (24). In the case of the CD-MPR, specific determinants in its cytoplasmic domain, in particular a casein kinase II phosphorylation site are required for high affinity interaction of AP-1 with TGN membranes (25).

In this study, we have investigated the sorting of the CD-MPR in polarized MDCK cells. For this, we have stably expressed a chimeric protein made of the luminal domain of the influenza virus hemagglutinin (HA) fused to the transmembrane and cytoplasmic tail of the CD-MPR. This HCD chimeric protein traffics within the basolateral domain and can be detected at the basolateral surface. Mutations of the different sorting signals proposed to mediate the interaction of the CD-MPR tail either with the Golgi-specific assembly proteins AP-1 or its plasma membrane counterpart AP-2 do not affect the basolateral delivery of the corresponding HCD chimeras. Truncations of the cytoplasmic domain indicated that a sorting determinant, unrelated to motifs necessary for clathrin-coated pit localization, is present in the membrane-proximal part of the CD-MPR cytoplasmic domain or the transmembrane domain, which confers basolateral targeting. This determinant, neither supports the AP-2-dependent endocytosis nor triggers the recruitment of AP-1 on membranes. This strongly suggests that an additional sorting machinery that recognizes signals unrelated to those mediating clathrin-coated pit localization could be responsible for basolateral targeting of membrane proteins in MDCK cells.

    EXPERIMENTAL PROCEDURES
Top
Abstract
Introduction
Procedures
Results
Discussion
References

Cell Culture-- MDCK cells (strain II) were grown as described (26). PA317 amphotropic retrovirus packaging cells (27) were maintained in MEM supplemented with 10% fetal calf serum, 4 mM glutamine, and antibiotics. All experiments, unless otherwise indicated, were performed with MDCK cells grown on 24.5-mm diameter, 0.4-mm pore size transwell units (Costar, Cambridge, MA). The polycarbonate filters were seeded with 0.5 to 1 × 106 cells each, and cell monolayers were used for experiments 4 days after plating.

HA and HA-MPR Constructs-- All manipulations of DNA were performed essentially as described in Ref. 28. To optimize expression of the chimeric proteins a Kozak consensus sequence was introduced into the cDNA encoding HA. Two complementary oligonucleotides with the sequences 5'-CAAGCTTGCCGCCACCATGG-3' and 5'-CCATGGTGGCGGCAAGCTTGGTAC-3' were ligated between the KpnI and MscI sites of plasmid pBHA (29) to create pBD16. To form pBD17 (wtHA), pBD16 was cleaved with HindIII and BamHI and the short fragment encoding the complete influenza virus hemagglutinin protein was cloned between EcoRI and BamHI sites of the retroviral vector pLXSN (30) after making the HindIII and EcoRI ends flush with Klenow. The construction of a chimeric gene, HCD, consisting of the luminal domain of the influenza virus HA fused to the transmembrane and cytoplasmic domains of the small MPR has been described previously (29). The chimeric gene HCD and all mutants derived thereof were inserted in pBD17 between the SalI and BamHI sites, thereby fusing the 5' end of HA, containing the Kozak consensus sequence, to all chimeric genes. The cytoplasmic domain truncation mutants (HCD-Delta 5 (pBD23), HCD-Delta 17 (pBD20), and HCD-Delta 55 (pBD26); see Fig. 1) and the substitution mutants (HCD-Delta 55,A-1 (pBD28) and HCD-A13A18A45 (pBD19)) were generated by oligonucleotide-directed mutagenesis of the wild type mouse CD-MPR cDNA (31) using the method of Kunkel (32). For the truncation mutants the codons for the CD-MPR cytoplasmic tail residues 63 (His) and 51 (Gln) were changed to stop codons. In the case of the substitution mutants, the last residue (Tyr) of the transmembrane domain and the cytoplasmic tail residues Phe13, Phe18, and Tyr45 were changed to alanines. The truncation mutant HCD-Delta 23 (pBD25) was generated by ligation of two complementary oligonucleotides with the sequences 5'-GCATGATTGG-3' and 5'-GATCCCAATCAT-3' between the PstI and BamHI sites of HCD, introducing a stop codon at Tyr45 in the cytoplasmic tail of the CD-MPR. Mutant HCD-Delta 55 (pBD26) and Delta 55,A-1 (pBD30) were constructed in a similar way using oligonucleotides with the sequences 5'-ATGGAGCAGTGAG-3' and 5'-GATCCTCACTGCTCCATTCCC-3'. This double-stranded DNA fragment was inserted between the BstXI and BamHI sites of HCD and HCD-A-1, respectively, creating a stop codon at residue 13 (Phe) of the cytoplasmic domain.

Stable Expression in MDCK Cells-- Infectious virus was generated for each of the constructs from the plasmid form of the retroviral vector by transient transfection of the packaging cells PA317 essentially as described (30). Virus-containing medium harvested from PA317 cells was used to infect MDCK cells. Stably transfected MDCK cells were selected in medium containing 0.8 mg/ml G418 (Life Technologies, Inc.) and cloned using glass cylinders. Cells expressing the protein of interest were identified by immunofluorescence. At least two independent clones expressing the highest levels of the respective chimeric protein were used for further experiments. All transfected cell lines used were fully polarized as judged by methionine uptake (basal/apical ratio greater than 4:1) (33) and secretion of an endogenous glycoprotein complex (34). Prior to experiments the tightness of monolayers was assessed with [3H]inulin (Amersham Corp.) (35). All experiments were performed using cell lines of passage numbers 5 through 10 after cloning.

Cell Surface Transport Assay-- MDCK cells grown on Costar Transwell units were rinsed with warm PBS++ (PBS containing 0.9 mM CaCl2 and 0.5 mM MgCl2) and starved for 60 min in MEM lacking cysteine and methionine (Select Amine-kit; Life Technologies, Inc.) containing 0.35 g/liter sodium bicarbonate, 20 mM Hepes, pH 7.3, and 0.5% BSA (MEM-BSA). Cells were then pulse labeled in a wet chamber for 20 min at 37 °C from the basolateral side with labeling medium (MEM-BSA supplemented with 2 mCi/ml Expre35S35S (1000 Ci/mmol, 10 mCi/ml) NEN Life Science Products). One set of filters was washed three times with cold PBS++ and placed on ice in MEM-BSA; the other sets of filters were chased at 37 °C in MEM-BSA with a 100-fold excess methionine and cysteine. At the end of the chase cells were washed with cold PBS++ and placed on ice in MEM-BSA. Subsequent steps were performed at 4 °C. Cells were washed once with MEM-BSA and incubated either from the apical or basolateral side with a 1:500 dilution of an hemagglutinin antiserum (monoclonal antibody H269, generous gift of J. Skehel) in MEM-BSA for 90 min on a rocking platform. In some experiments the antibody was included in the apical or basolateral chase medium and allowed to bind for another 90 min on ice. The excess unbound antibodies were removed over 30 min by three washes with MEM-BSA and one wash with PBS++, filters were cut out of the holder, and cells were lysed in the presence of an excess of unlabeled protein. (For preparation of unlabeled lysates a cell line overexpressing the wild type HA was grown to confluency on 10-cm culture dishes. Cells from one dish were lysed on ice in 2.5 ml of B1 (50 mM Tris, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS containing freshly added protease inhibitor mixture (2 mg/ml leupeptin, 2 mg/ml aprotinin, 1 mM benzamidine, 1 mM phenylmethylsulfonyl fluoride)). The lysate was cleared by centrifugation for 5 min at 12,000 × g, and 1 ml of supernatant was used to lyse labeled cells.) The lysate was centrifuged in an Eppendorf microfuge for 5 min to remove debris. An aliquot (one-tenth of the lysate) was supplemented with additional antibody (anti-HA, mAb H269) and incubated overnight at 4 °C, and total labeled protein was isolated by the addition of protein A-Sepharose. The second aliquot (nine-tenths of the lysate) was frozen in liquid nitrogen and stored overnight at -70 °C. Labeled protein that had appeared on the cell surface and thus had bound antibody was precipitated by the addition of protein A-Sepharose. Precipitates were washed three times with B1, twice with B2 (50 mM Tris, pH 7.2, 100 mM NaCl, 2 mM EDTA, 0.1% Triton X-100, 0.5% SDS, 0.5% deoxycholate), twice with B3 (50 mM Tris, pH 7.2, 500 mM NaCl, 2 mM EDTA, 0.1% Triton X-100), and once with B4 (50 mM Tris, pH 7.2, 100 mM NaCl, 2 mM EDTA). Finally, proteins were released from the beads by boiling in Laemmli sample buffer and analyzed by SDS-PAGE on a 10% polyacrylamide gel (36). The band intensities were calculated using a PhosphorImager (Molecular Dynamics, Sunnyvale, CA), and the amount of protein transported to the cell surface was expressed as the percentage of the total immunoprecipitated protein. In some experiments cells were pretreated with 10 mM butyric acid (Sigma) 12 h prior to labeling to induce transcription of stably transfected cDNA constructs (8).

Sucrose Velocity Gradient Centrifugation-- MDCK cells grown on 60-mm plastic dishes to subconfluency and treated with 10 mM NH4Cl 12-16 h prior to the experiment to reduce the endogenous level of lysosomal proteases were metabolically labeled essentially as described above except that cells were labeled for 1 h and chased for 3 h. After labeling cells were lysed, and an aliquot of the lysate was analyzed by sucrose gradient centrifugation as described (37). Gradient fractions were immunoprecipitated with anti-HA and analyzed by SDS-PAGE and fluorography.

Internalization Assay Based on Surface Biotinylation-- Internalization rates of CD-MPR chimeras were determined by the surface biotinylation assay described in Ref. 38, except that MESNa was used for stripping of the cell surface biotin. Biotinylated CD-MPR chimera were detected by Western blotting with 125I-labeled streptavidin. Signals were quantified using a PhosphorImager.

AP-1 Recruitment-- HeLa cells were grown on coverslips in alpha -MEM supplemented with 10% fetal calf serum, 10 mM Hepes, 2 mM glutamine, 100 units/ml penicillin, and 100 µg/ml streptomycin. The cells were washed in medium devoid of serum and then incubated for 30 min with a recombinant virus that expresses the T7 polymerase gene (39). After washing the cells in medium supplemented with 5 mM hydroxyurea, the N-[1(2,3-dioleoyloxy)propyl]-N,N,N-trimethyl-ammonium-methylsulfate reagent (Boehringer Mannheim) was used to transfect the cells with the indicated constructs (HCD and HCD-Delta 55) cloned in pGEM1 vector, following the manufacturer's instructions. After 1 h, the cells were washed and allowed to express for 2-3 h in medium supplemented with 5 mM hydroxyurea. Pulse-chase experiments performed in parallel indicated that similar levels of HCD and HCD-Delta 55 were expressed under those conditions and transported through the secretory pathway with similar kinetics (data not shown). Cells were then fixed for 20 min with 3% paraformaldehyde, permeabilized with 0.1% Triton X-100 for 5 min, and subsequently incubated with the monoclonal 100/3 anti-gamma -adaptin antibody (kindly provided by E. Ungewickell) and a rabbit polyclonal anti-HA antibody for 30 min at room temperature. The bound antibodies were detected with fluorescein isothiocyanate or Texas Red-conjugated secondary antibodies (Dianova).

For quantitation of the fluorescent signal, randomly chosen fields were captured using a cooled charged and coupled device CH250 from Photometrics (Tuckson, AZ) having a Kodak KAF 1400 CCD CHIP (grade 2) for 12-Bit image collection that was controlled by the KHOROS software package running on a SUN 10/41 workstation (Silicon Graphics, USA). In every field, regions corresponding to the TGN area of transfected or nontransfected cells were selected, and the fluorescence in the Texas Red channel corresponding to the 100/3 monoclonal antibody was calculated using the AIM program developed by J. C. Olivo from the EMBL microcomputing and data acquisition group. After calculating the fluorescence intensities (mean intensity/pixel or total intensity) from 88 (HCD transfected), 122 (nontransfected), and 73 (HCD-Delta 55) cells, mean and standard error were calculated. The confidence limits of the results obtained were assessed by Student's t test.

    RESULTS
Top
Abstract
Introduction
Procedures
Results
Discussion
References

In MDCK cells, the Man-6-P/IGF II receptor traffics between the TGN and the endocytic organelles of the basolateral domain and is found on the basolateral plasma membrane (16). To study the transport of the CD-MPR in these cells, we have generated MDCK clones stably expressing chimeric proteins made of the luminal domain of the influenza virus HA, an apically sorted membrane glycoprotein, fused to the transmembrane and cytoplasmic domain of this receptor (Fig. 1) and performed pulse-chase experiments followed by cell surface immunoprecipitations to measure the appearance of these chimeric proteins on the apical or the basolateral domain.


View larger version (14K):
[in this window]
[in a new window]
 
Fig. 1.   Schematic representation of the wild type HA, the wild type CD-MPR (CD), and the HA/CD-MPR chimera (HCD). HCD consists of the luminal domain of the hemagglutinin (open box) and the transmembrane domain (striped box) and cytoplasmic domain (stippled box) of the CD-MPR. The amino acid sequence of the transmembrane and cytoplasmic domain of HCD is enlarged. The transmembrane domain sequence is overlined. Arrows indicate the positions of carboxyl-terminal truncations (Delta  indicates the number of residues deleted) and point mutations (labeled with the code for the introduced amino acid and the position of the replacement).

HA/CD-MPR Chimeras Are Normally Transported through the Secretory Pathway and Are Present on the Basolateral Membrane of MDCK Cells-- The normal transport of HA to the cell surface depends on its proper folding and trimerization (37). Because modifications in the transmembrane and cytoplasmic domains of HA can affect its normal rate of transport (40, 41) we determined whether the HA/CD-MPR chimera (HCD construct) is properly folded and transported through the secretory pathway. We first performed classical pulse-chase experiments on MDCK cells expressing either the wild type HA or the HCD and immunoprecipitated these labeled proteins using a monoclonal antibody directed against the luminal domain of HA. After a 20-min pulse, the HA and the HCD were found in low molecular weight, immature forms that were rapidly converted upon a chase into higher molecular weight, mature forms reflecting the conversion of high mannose to complex type oligosaccharides (Fig. 2). These results indicate that the HCD chimera moves efficiently through the secretory pathway with similar rates as the HA (t1/2 approx  30 and 25 min, respectively). Such pulse-chase experiments were also performed with the HCD-Delta 55, which harbors a 55-amino acid-long truncation of the CD-MPR tail (Fig. 2). The rate of transport of this mutant was slightly slower than that of HCD (t1/2 approx  45 min), indicating that the deletion of the CD-MPR tail only moderately affects the transport of the corresponding chimera. The other mutants used in this study were transported through the secretory pathway with similar rates as that of HCD-Delta 55 (data not shown). The oligomeric state of HCD and HCD-Delta 55 were analyzed by centrifugation of detergent extracts on sucrose density gradients (37). After the pulse, HCD and HCD-Delta 55 immunoprecipitated from the different fractions distributed in a single peak, as the monomeric HA (Fig. 3). After a chase period, however, both chimeric proteins with complex type sugars were recovered in denser fractions, showing a similar profile as the mature trimeric HA. A slightly higher percentage of the HCD-Delta 55 remains in the lighter density fractions in comparison with HA or HCD following a 3-h chase. This suggests that the rate of oligomerization of the trucation mutant is slightly slower than that of HA or HCD. Nevertheless, these results show that both chimeric proteins acquire the same trimeric conformation as HA.


View larger version (20K):
[in this window]
[in a new window]
 
Fig. 2.   Intracellular transport of wild type HA and HA/CD-MPR chimeras in MDCK cells. Transfected cells were pulse labeled with [35S]methionine/cysteine for 20 min and chased for various periods of time as indicated. Cells were solubilized with Triton X-100 and HA, and the HA/CD-MPR chimeras were immunoprecipitated with the anti-HA monoclonal antibody, resolved by SDS-PAGE, and visualized by fluorography as described previously (25). Duplicates are shown for each time point. Fluorographs were quantified by PhosphorImager. The kinetics of maturation of the HA and the HA/CD-MPR chimeras is represented by the disappearance of the precursor (immature) form calculated as the percentage of the precursor present at t = 0.


View larger version (44K):
[in this window]
[in a new window]
 
Fig. 3.   Oligomerization of wild type HA and HA/CD-chimeras. Transfected cells were either pulse labeled with [35S]methionine/cysteine for 1 h (P) or pulse labeled for 1 h followed by a 3-h chase (C). Cell lysates were centrifuged on 5-25% (w/v) sucrose gradients containing 0.1% Triton X-100. Each fraction was immunoprecipitated with anti-HA and analyzed by SDS-PAGE and fluorography.

We next performed cell surface immunoprecipitations on biosynthetically labeled MDCK cells grown on filters to monitor the appearance of the newly synthesized wild type HA and HCD on the apical or the basolateral surface (Fig. 4). As found previously (35, 42) the bulk of the wild type HA was transported to and accumulated at the apical plasma membrane. Most of the newly synthesized HCD, however, progressively appeared on the basolateral plasma membrane. Typically, 90% of the cell surface HCD was found on the basolateral plasma membrane after a 40-min chase (Table I). As observed for the MPRs, the amount of the cell surface HCD was low due to its capacity to be endocytosed. Using an internalization assay based on cell surface biotinylation, the endocytosis rate of HCD was found to be approx 2.6%/min (Table I). Because only minor amounts of HCD could be detected at any time on the apical surface even when the antibody was present during the chase, we conclude that HCD is vectorially transported from the TGN for trafficking within the basolateral domain. These results suggest that either the transmembrane or the cytoplasmic domain of the CD-MPR contains a dominant basolateral sorting determinant.


View larger version (19K):
[in this window]
[in a new window]
 
Fig. 4.   Cell surface delivery of wild type HA and the HCD in MDCK cells. Filter grown MDCK cells expressing wild type HA or the HCD were pulse labeled with [35S]methionine/cysteine for 20 min and subsequently chased for 0-80 min. At each time point anti-HA was added to the apical (A) or basolateral (B) medium and allowed to bind on ice. Cells were lysed, and one-tenth of the sample was removed to determine the total amount of labeled HA or HCD. The rest of the lysate was incubated with protein A-Sepharose beads, and bound proteins were analyzed by SDS-PAGE and fluorography. Fluorographs were quantified by PhosphorImager, and the values for recovered cell surface protein were expressed as the percentage of total mature protein.

                              
View this table:
[in this window]
[in a new window]
 
Table I
Polarized surface expression and internalization of HA/CD-MPR chimeras

Mutations of Endocytosis Sorting Signals Do Not Affect Basolateral Transport of HA/CD-MPR Chimeras-- To decipher the determinants important for sorting to the basolateral domain, we introduced several deletions and point mutations in the CD-MPR cytoplasmic tail (Fig. 1). The mutations were designed to affect the sequences known to be important for intracellular trafficking of the CD-MPR. We first deleted the carboxyl-terminal di-leucine motif alone (construct HCD-Delta 5) or together with the adjacent casein kinase II phosphorylation site (HCD-Delta 17), shown to be critical for efficient lysosomal enzyme targeting (18, 25). Table I shows that after a 40-min chase, 80% of these mutant proteins present on the cell surface were still detected on the basolateral plasma membrane. Pulse-chase experiments followed by cell surface immunoprecipitation indicated that the mutant proteins were vectorially transported from the Golgi complex to the basolateral domain (data not shown). The endocytosis of the CD-MPR is mediated by two independent endocytosis motifs, a dominant determinant containing phenylalanine 13 and 18 and a weak determinant containing the tyrosine 45 (17). We therefore replaced these three critical amino acids in the HA/CD-MPR chimera by alanine residues (construct HCD-A13A18A45). Indeed, these mutations significantly reduced the endocytosis rate of the chimera by 60% (Table I). However, the sorting of this mutant was not affected, and 90% of the cell surface protein was still found on the basolateral plasma membrane. Therefore, it appears that neither the endocytosis motifs nor the sequence important for lysosomal enzyme delivery are essential for basolateral transport when mutated individually.

We introduced further truncations in the CD-MPR cytoplasmic domain to remove both determinants. The chimeric proteins in which the sequences containing the tyrosine 45 (construct HCD-Delta 23) or both the tyrosine 45 and the phenylalanine 13 and 18 (construct HCD-Delta 55) were removed also targeted predominantly to the basolateral domain (more than 80%, Table I). A similar cell surface distribution was found for the construct HCD-Delta 17,A13A18A45. Therefore, none of the determinants involved either in endocytosis or in lysosomal enzyme targeting are essential for basolateral sorting of the HA/CD-MPR chimera. Examination of the CD-MPR tail and transmembrane domain of the most truncated HA/CD-MPR revealed the presence of a tyrosine residue (Tyr-1) contained within a sequence motif, YQRL, that resembles a classical coated pit localization signal (43). To test whether this tyrosine was essential for basolateral targeting of the most truncated HA/CD-MPR chimera, this residue was mutated into an alanine (construct HCD-Delta 55,A-1). As shown in Table I, this mutant protein was mostly targeted to the basolateral domain (more than 80%). Together, these results indicate that an additional signal(s), unrelated to those mediating clathrin-coated pit localization, must be present to mediate basolateral transport of the most truncated form of the HA/CD-MPR chimera.

Basolateral Sorting of HCD-Delta 55 Is Saturable-- Signal-mediated sorting of membrane proteins in the secretory pathway of MDCK cells can be saturated by overexpressing these membrane proteins. This has been observed for the newly synthesized lgp 120 (10) or LDL receptor (8). To test the possibility that basolateral sorting of the most truncated HCD construct was signal-mediated and could become saturated, we selected MDCK clones highly expressing the HCD-Delta 55,A-1 construct (approx 7-fold overexpression compared with HCD) and performed pulse-chase experiments followed by cell surface immunoprecipitation. Fig. 5 shows that high levels of expression of this mutant protein resulted in a significant missorting to the apical domain when compared with lower expression levels. Thus, there is a clear correlation between overexpression of this mutant and apical delivery. The appearance on both plasma membrane domains occurred at similar initial rates, strongly suggesting that the missorting of this truncation mutant occurred in the trans-Golgi network. These results suggest that the 12-amino acid-long sequence adjacent to the CD-MPR transmembrane domain and/or the CD-MPR transmembrane domain contains a determinant for basolateral transport and that the corresponding sorting machinery could become saturated upon overexpression of this chimera.


View larger version (9K):
[in this window]
[in a new window]
 
Fig. 5.   Effect of expression level of truncated HA/CD-MPR chimera on cell surface delivery. Cell surface delivery of the truncated HA/CD-MPR chimera (HCDDelta 55,A-1) was determined in low (A) and high (B) expressing MDCK cells using the cell surface immoprecipitation assay described in the legend to Fig. 4. Expression levels of the HA/CD-MPR chimera in low expressing MDCK cells was comparable with that of the endogenous Man-6-P/IGF II receptor, whereas the highly expressing MDCK cells showed a 7-fold overexpression.

HCD-Delta 55 Tail Does Not Interact with AP-1-- In nonpolarized and polarized cells, the MPRs are sorted from the TGN via the Golgi-specific assembly proteins AP-1-dependent pathway. The recruitment of AP-1 on membranes can be triggered by the expression of the MPRs (21-23). To test the possibility that the HCD-Delta 55 could still be sorted along an AP-1-dependent pathway, we overexpressed HCD-Delta 55 as well as HCD and monitored the recruitment of AP-1 by immunofluorescence. Several established cell lines, such as baby hamster kidney and Chinese hamster ovary cells, have been recently shown to contain apical and basolateral cognate routes from the TGN to the plasma membrane (3). These experiments were performed in the nonpolarized HeLa cells, which most likely exhibit such cognate routes. Fig. 6 shows that HeLa cells overexpressing HCD exhibit, as expected, an increase in AP-1 staining in the perinuclear region when compared with mock transfected cells. However, AP-1 staining in cells overexpressing similar amounts of HCD-Delta 55 was indistinguishable from that of mock transfected cells. Quantitation of these experiments indicated that expression of HCD induced a 2-fold increase in AP-1 binding, whereas overexpression of HCD-Delta 55 remained without any effect (Fig. 7). We conclude from these results that the HCD-Delta 55 chimera is unable to recruit AP-1 on Golgi membranes. Therefore, its basolateral targeting in MDCK cells does not involve the AP-1-dependent pathway and may require an additional sorting machinery.


View larger version (72K):
[in this window]
[in a new window]
 
Fig. 6.   AP-1 immunostaining in HeLa cells overexpressing different HA-CDMPR constructs. HeLa cells were infected with a recombinant T7 RNA polymerase vaccinia virus and transfected with cDNAs encoding the HCD chimera (a and b) or the mutant HCD-Delta 55 (c and d). After 3 h of expression, cells were fixed and labeled with the 100/3 monoclonal anti-gamma -adaptin antibody (a and c) and the polyclonal anti-HA antibody (b and d) as indicated under "Experimental Procedures." (Representative fields, the nontransfected cells are indicated with asterisks.)


View larger version (18K):
[in this window]
[in a new window]
 
Fig. 7.   Quantitation of the amount of gamma -adaptin associated with the TGN in cells overexpressing the wild type or mutant version of the HCD chimeras. HeLa cells were transiently transfected as in Fig. 6 and similarly processed for double indirect immunofluorescence. The intensity of the fluorescence signal corresponding to gamma -adaptin (Texas Red channel) was quantitated for 122 cells (nontransfected cells), 88 cells (HCD), and 73 cells (HCD-Delta 55) cells, and the results were processed as described under "Experimental Procedures." Values correspond to the means ± S.E. of four independent experiments (expression of the chimera for 2 or 3 h). Using the Student's t test, the confidence limits of the sample populations were found to be higher than 99% for HCD and not significantly different for HCD-Delta 55 when compared with nontransfected cells.

    DISCUSSION
Top
Abstract
Introduction
Procedures
Results
Discussion
References

We have expressed in the MDCK cells chimeric proteins made of the transmembrane and cytoplasmic domain of the CD-MPR fused to the luminal domain of the apically directed hemagglutinin to study CD-MPR trafficking in polarized cells. We show that 1) the chimeric protein traffics within the basolateral domain, 2) the mutagenesis of determinants related to clathrin-coated pit localization signals in the CD-MPR tail does not affect its appearance on the basolateral membrane, 3) an additional sorting determinant is present in the cytoplasmic domain and/or the transmembrane domain of CD-MPR that mediates basolateral transport, and 4) this basolateral sorting determinant is not efficiently recognized by the Golgi-specific AP-1 assembly proteins and therefore may involve a different sorting machinery.

Our previous work in nonpolarized cells has shown that HA/MPR chimeras colocalize with the endogenous MPRs, indicating that the information contained within these chimeric proteins is sufficient to specify their intracellular localization (29). We have now expressed these HA/CD-MPR chimeras in polarized MDCK cells. Cell surface immunoprecipitation experiments revealed that more than 90% of the cell surface chimera is present at the basolateral plasma membrane indicating that this protein traffics within the basolateral domain of MDCK cells. This result agrees with that of Breuer and co-workers (44), who showed that the CD-MPR is predominantly present on the basolateral plasma membrane of MDCK cells. Thus, both the CD-MPR and the Man-6-P/IGF II receptor (16) traffic within the same cellular domain of MDCK cells. This HCD chimera is able to trigger the recruitment of the AP-1, Golgi-specific assembly proteins on membranes. Therefore, it is likely that its appearance on the basolateral membrane reflects its efficient sorting to endosomes along the AP-1-dependent pathway followed by the recycling of a fraction of this chimeric protein back to the basolateral plasma membrane.

To identify the minimal determinant required for this basolateral sorting, we first mutagenized the signals in the CD-MPR cytoplasmic domain known to be important for its endocytosis (phenylalanine 13 and 18 and tyrosine 45), efficient lysosomal enzyme targeting (carboxyl-terminal di-leucine motif), and high affinity binding of AP-1 on membranes (the casein kinase II phosphorylation site adjacent to the carboxyl-terminal di-leucine motif). The re-expression of CD-MPR mutants in MPR-negative fibroblasts has indicated that the mutation of either the carboxyl-terminal di-leucine motif alone or the endocytosis motif alone (phenylalanine 13 and 15 and tyrosine 45) does not affect the efficient AP-1 recruitment (25). Consistent with this notion, the same mutations introduced in the HCD chimera do not affect its basolateral delivery. This suggests that the protein is still able to follow the AP-1-dependent pathway in MDCK cells. The most striking finding is that mutations in the CD-MPR cytoplasmic domain removing the different clathrin-coated pit localization signals do not alter the basolateral delivery of the corresponding HCD mutant (HCD-Delta 17,A13A18A45). Even the largest truncation of the CD-MPR tail did not result in the apical delivery of the truncation mutant (HCD-Delta 55). Such an HCD mutant cannot trigger the recruitment of AP-1 on Golgi membranes nor can it be endocytosed at the plasma membrane. Therefore, it appears that this membrane protein devoid of clathrin-coated pit localization signals is transported to the basolateral domain of MDCK cells via an AP-1-independent pathway.

Basolateral sorting of membrane proteins generally relies on signals located in their cytoplasmic domains. We therefore examined the 12 amino acids left in the CD-MPR cytoplasmic tail of HCD-Delta 55 for the presence of putative sorting signals (Fig. 8). First, a YQRL motif is present that is identical to that found in TGN 38. This determinant was shown to be important for the retrieval of TGN 38 from the plasma membrane to the TGN in nonpolarized cells (45, 46) and basolateral sorting in MDCK cells (47). Hydrophobicity plots of the CD-MPR predict that the tyrosine residue in this YQRL motif is the last amino acid of the transmembrane domain (17) and that this motif would be barely accessible to the sorting machinery. However, it has been noticed that Golgi-resident proteins have on average shorter transmembrane domains (approx 15 residues) than plasma membrane proteins (approx 20 residues) (48, 49). Because the CD-MPR transmembrane domain is 20-25 amino acids long, it remained possible that in the context of the Golgi membrane the tyrosine contained in this YQRL motif could be accessible to the basolateral sorting machinery. A 15-amino acid-long transmembrane domain could provide the minimal spacing for accessibility of this tyrosine-based motif. For example, the basolateral sorting of the beta -amyloid precursor is dependent upon a key tyrosine residue in the cytoplasmic tail of the protein located only 5 residues away from the transmembrane domain (50). However, mutation of the tyrosine residue to an alanine in our most truncated HCD chimera (HCD-Delta 55,A-1) did not influence its basolateral transport at low levels of expression. Therefore, this tyrosine-based motif does not appear to be essential for basolateral transport of the most truncated mutant.


View larger version (17K):
[in this window]
[in a new window]
 
Fig. 8.   Sequence comparison of the cytoplasmic domains of different basolaterally targeted membrane proteins. Sequences are shown in single-letter code. Triangles mark the first residue of the cytoplasmic domains, and asterisks mark the last residue. The cytoplasmic domain of the most truncated HCD chimera (HCD-Delta 55) is aligned with the cytoplasmic tails of the beta -amyloid precursor and TGN 38 based on its YQRL motif and with the polyIgR and cation-independent MPR cytoplasmic tails based on the RXXV motif. Tyrosine residues in the clathrin-coated pit localization motifs are highlighted with ovals, and the arginine and valine residues in the RXXV motifs are highlighted with squares.

A basolateral sorting determinant could reside in the RLVV sequence overlapping the YQRL motif (Fig. 8). A similar motif (HRRNV), unrelated to classical coated pit localization signals and closely located near the transmembrane domain, has been shown to mediate basolateral delivery of a truncated version of the poly-Ig receptor (13). Within this sequence motif the histidine, the first arginine, and the carboxyl-terminal valine are most essential for basolateral sorting. Two of three essential residues (arginine and valine) are conserved in the CD-MPR membrane-proximal determinant. Interestingly, the Man-6-P/IGF II receptor cytoplasmic domain also contains a similar sequence motif (RETV) close to the transmembrane segment (Fig. 8). Because further truncations of the 12-residue-long tail of the HCD chimera resulted in retention of the protein in the endoplasmic reticulum (data not shown), we cannot exclude the remote possibility that the transmembrane domain of the CD-MPR contributes to basolateral delivery. A more likely explanation, however, is that these 12 amino acids contain a determinant (possibly the RLVV motif) that mediates basolateral delivery of HCD in the absence of the known clathrin-coated pit localization signals. The final characterization of this signal awaits more extensive mutational analysis. If this sorting determinant mediates the basolateral delivery of the luminal domain of HA used as a reporter, it also remains to be determined to what extent it contributes to CD-MPR trafficking.

It is interesting to note that the overexpression of the most truncated HCD-Delta 55 mutant results in the significant delivery of the protein to the apical membrane. This observation was not only made with high expressing MDCK clones (this study) but also with low expressing MDCK clones treated with butyrate, which induces a 5-10-fold increase in the expression level (data not shown). HCD-Delta 55 or HCD-Delta 55,A-1 were significantly missorted to the apical plasma membrane after butyrate-induced overexpression. Under such conditions, 40% of the cell surface proteins were present on the apical membrane, strongly suggesting that a saturable, AP-1-independent transport machinery recognizes a basolateral sorting determinant present in the 12-amino acid-long cytoplasmic tail and/or transmembrane domain of CD-MPR. A similar result was also obtained with the HCD-Delta 17,A13A18A45 mutant. In contrast, such an increase in the expression level of HCD, which follows an AP-1-dependent pathway at the exit of the TGN, had almost no effect on its basolateral delivery. Thus, these two sorting machineries can be saturated in a different manner. Little is known about the machineries responsible for basolateral delivery of membrane proteins in polarized cells. Similarities between several basolateral and clathrin-coated pit localization signals have suggested that basolateral transport of membrane proteins requires coat components related to the assembly proteins of clathrin-coated vesicles. Thus far, the µ subunits of the AP-1 and AP-2 assembly proteins have been shown to interact with tyrosine-based motifs in the yeast two hybrid system (51, 52). In a similar manner, the µ subunit of the newly described AP-3 complex that shares structural similarities with AP-1 and AP-2 also recognizes tyrosine-based sorting signals (52). Several basolateral targeted membrane proteins have been shown to contain tyrosine-independent sorting determinants. It remains to be determined whether these tyrosine-independent sorting determinants can be recognized by coat components related or unrelated to the AP-1 and AP-2 assembly proteins.

    ACKNOWLEDGEMENTS

We thank Dr. J. Skehel for the anti-HA monoclonal antibody, Dr. E. Ungewickell for the 100/3 anti-gamma -adaptin monoclonal antibody, and Dr. M.-J. Getting for providing the HA cDNA.

    FOOTNOTES

* This work was supported in part by funds from Vaincre les Maladies Lysosomales and the European communities (to B. D.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Present address: Dept. of Biochemistry, Academic Medical Center, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.

§ To whom correspondence should be addressed. Present address: Institut de Biologie de Lille, EP CNRS 525, Institut Pasteur de Lille, BP 447, 1 rue Calmette, 59021 Lille Cedex, France. Tel.: 33-3-20-87-10-25; Fax: 33-3-20-87-10-19; E-mail: bernard.hoflack{at}Pasteur-Lille.fr.

1 The abbreviations used are: MDCK, Madin-Darby canine kidney; TGN, trans-Golgi network; MPR, mannose 6-phosphate receptor; CD-MPR, cation-dependent mannose 6-phosphate receptor; HA, hemagglutinin; IGF, insulin-like growth factor; PAGE, polyacrylamide gel electrophoresis; Man-6-P, mannose 6-phosphate; MEM, minimum Eagle's medium; PBS, phosphate-buffered saline; BSA, bovine serum albumin.

    REFERENCES
Top
Abstract
Introduction
Procedures
Results
Discussion
References

  1. Wandering-Ness, A., Bennett, M. K., Antony, C., and Simons, K. (1990) J. Cell Biol. 111, 987-1000[Abstract]
  2. Ikonen, E., Tagaya, M., Ullrich, O., Montecucco, C., and Simons, K. (1995) Cell 81, 571-580[Medline] [Order article via Infotrieve]
  3. Yoshimori, T., Keller, P., Roth, M. G., Simons, K. (1996) J. Cell Biol. 133, 247-256[Abstract]
  4. Brown, D. A., Crise, B., and Rose, K. (1989) Science 245, 1499-1501[Medline] [Order article via Infotrieve]
  5. Lisanti, M. P., Caras, I. W., Davitz, M. A., Rodriguez-Boulan, E. (1989) J. Cell Biol. 109, 2145-2156[Abstract]
  6. Scheiffele, P., Peränen, J., and Simons, K. (1995) Nature 378, 96-98[CrossRef][Medline] [Order article via Infotrieve]
  7. Matter, K., and Mellman, I. (1994) Curr. Opin. Cell Biol. 6, 545-554[Medline] [Order article via Infotrieve]
  8. Matter, K., Hunziker, W., and Mellman, I. (1992) Cell 71, 741-753[Medline] [Order article via Infotrieve]
  9. Thomas, D. N. C., Brewer, C. B., and Roth, M. G. (1993) J. Biol. Chem. 268, 3313-3320[Abstract/Free Full Text]
  10. Höning, S., and Hunziker, W. (1995) J. Cell Biol. 128, 321-332[Abstract]
  11. Hunziker, W., and Fumey, C. (1994) EMBO J. 13, 2963-2969[Abstract]
  12. Casanova, J. E., Apodaca, G., and Mostov, K. (1991) Cell 66, 65-75[Medline] [Order article via Infotrieve]
  13. Aroeti, B., Kosen, P. A., Kuntz, I. D., Cohen, F. E., Mostov, K. E. (1993) J. Cell Biol. 123, 1149-1160[Abstract]
  14. Aroeti, B., and Mostov, K. (1994) EMBO J. 13, 2297-2304[Abstract]
  15. Matter, K., Whitney, J. A., Yamamoto, E. M., Mellman, I. (1993) Cell 74, 1053-1064[Medline] [Order article via Infotrieve]
  16. Prydz, K., Brändli, A. W., Bomsel, M., and Simons, K. (1990) J. Biol. Chem. 265, 12629-12635[Abstract/Free Full Text]
  17. Johnson, K. F., Chan, W., and Kornfeld, S. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 10010-10014[Abstract]
  18. Johnson, K. F., and Kornfeld, S. (1992) J. Biol. Chem. 267, 17110-17115[Abstract/Free Full Text]
  19. Johnson, K. F., and Kornfeld, S. (1992) J. Cell Biol. 119, 249-257[Abstract]
  20. Chen, H. J., Remmler, J., Delaney, J. C., Messner, D. J., Lobel, P. (1993) J. Biol. Chem. 268, 22338-22346[Abstract/Free Full Text]
  21. Le Borgne, R., Schmidt, A., Mauxion, F., Griffiths, G., and Hoflack, B. (1993) J. Biol. Chem. 268, 22552-22556[Abstract/Free Full Text]
  22. Le Borgne, R., Griffiths, G., and Hoflack, B. (1996) J. Biol. Chem. 271, 2162-2170[Abstract/Free Full Text]
  23. Le Borgne, R., and Hoflack, B. (1997) J. Cell Biol. 137, 335-345[Abstract/Free Full Text]
  24. Stamnes, M. A., and Rothman, J. E. (1993) Cell 73, 999-1005[Medline] [Order article via Infotrieve]
  25. Mauxion, F., Le Borgne, R., Munier-Lehmann, H., and Hoflack, B. (1996) J. Biol. Chem. 271, 2171-2178[Abstract/Free Full Text]
  26. Matlin, K. S., Reggio, H., Helenius, A., and Simons, K. (1981) J. Cell Biol. 91, 601-613[Abstract/Free Full Text]
  27. Miller, A. D., and Buttimore, C. (1989) Mol. Cell. Biol. 6, 2895-2901
  28. Sambrook, J, Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
  29. Mauxion, F., Schmidt, A., Le Borgne, R., Hoflack, B. (1995) Eur. J. Cell Biol. 66, 119-126[Medline] [Order article via Infotrieve]
  30. Miller, A. D., and Rosman, G. J. (1989) BioTechniques 7, 980-990 [Medline] [Order article via Infotrieve]
  31. Ludwig, T., Ruther, U., Metzger, R., Copeland, N. G., Jenkins, N. A., Lobel, P., Hoflack, B. (1992) J. Biol. Chem. 267, 12211-12219[Abstract/Free Full Text]
  32. Kunkel, T. A. (1985) Proc. Natl. Acad. Sci. U. S. A. 82, 488-492[Abstract]
  33. Balcarova-Stander, J., Pfeiffer, S. E., Fuller, S. D., Simons, K. (1984) EMBO J. 3, 2687-2694[Abstract]
  34. Urban, J., Parczyk, K., Leutz, A., Kayne, M., and Kondor-Koch, C. (1987) J. Cell Biol. 105, 2735-2743[Abstract]
  35. Brewer, C. B., and Roth, M. G. (1991) J. Cell Biol. 114, 413-421[Abstract]
  36. Laemmli, U. K. (1970) Nature 227, 680-685[Medline] [Order article via Infotrieve]
  37. Copeland, C. S., Doms, R. W., Bolzau, E. M., Webster, R. G., Helenius, A. (1986) J. Cell Biol. 103, 1179-1191[Abstract]
  38. Prill, V., Lehmann, L., Von Figura, K., Peters, C. (1993) EMBO J. 12, 2181-2193[Abstract]
  39. Fuerst, T. R., Niles, E. G., Studier, F. W., Moss, B. (1986) Proc. Natl. Acad. Sci. U. S. A. 83, 8122-8126[Abstract]
  40. Doyle, C., Roth, M. G., Sambrook, J., and Gething, M.-J. (1985) J. Cell Biol. 100, 704-714[Abstract]
  41. Doyle, C., Sambrook, J., and Gething, M.-J. (1986) J. Cell Biol. 103, 1193-1204[Abstract]
  42. Matlin, K. S., and Simons, K. (1984) J. Cell Biol. 99, 2131-2139[Abstract]
  43. Trowbridge, I. S., Collawn, J. F., and Hopkins, C. R. (1993) Annu. Rev. Cell Biol. 9, 129-161[CrossRef]
  44. Breuer, P., Körner, C., Böker, C., Herzog, A., Pohlmann, R., and Braulke, T. (1997) Mol. Biol. Cell 8, 567-576[Abstract]
  45. Bos, K., Wraight, C., and Stanley, K. K. (1993) EMBO J. 12, 2219-2228[Abstract]
  46. Humphrey, J. S., peters, P. J., Yuan, L. C., Bonifacino, J. S. (1993) J. Cell Biol. 120, 1123-1135[Abstract]
  47. Rajasekaran, A. K., Humphrey, J. S., Wagner, M., Miesenbock, G., Le Bivic, A., Bonifacino, J. S., Rodriguez-Boulan, E. (1994) Mol. Biol. Cell 5, 1093-1103[Abstract]
  48. Bretscher, M. S., and Munro, S. (1993) Science 261, 1280-1281[Medline] [Order article via Infotrieve]
  49. Pelham, H. R. B., and Munro, S. (1993) Cell 75, 603-605[Medline] [Order article via Infotrieve]
  50. Haass, C., Koo, E. H., Capell, A., Teplow, D. B., Selkoe, D. J. (1995) J. Cell Biol. 128, 537-547[Abstract]
  51. Boll, W., Ohno, W., Songyang, Z., Rapoport, I., Cantley, L. C., Bonifacino, J. S., Kirchhausen, T. (1996) EMBO J. 15, 5789-5795[Abstract]
  52. Ohno, H., Stewart, J., Fournier, M. C., Bosshart, H., Rhee, I., Miyatake, T., Saito, T., Galluser, A., Kirchhausen, T., Bonifacino, J. S. (1995) Science 269, 1872-1875[Medline] [Order article via Infotrieve]


Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.