From the Department of Biochemistry, University of
Texas Health Center, Tyler, Texas 75710, § Department of
Biological Resource Sciences, Miyazaki University, Miyazaki 889-21, Japan, and ¶ Department of Biochemistry, Miyazaki Medical College,
Kiyotake, Miyazaki 889-16, Japan
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ABSTRACT |
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Human monoamine (M)-form and simple phenol (P)-form phenol sulfotransferases (PSTs), which are greater than 93% identical in their primary sequences, were used as models for investigating the structural determinants responsible for their distinct substrate specificity and other enzymatic properties. A series of chimeric PSTs were constructed by reciprocal exchanges of DNA segments between cDNAs encoding M-form and P-form PSTs. Functional characterization of the recombinant wild-type M-form, P-form, and chimeric PSTs expressed in Escherichia coli and purified to homogeneity revealed that internal domain-spanning amino acid residues 84-148 contain the structural determinants for the substrate specificity of either M-form or P-form PST. Data on the kinetic constants (Km, Vmax, and Vmax/Km) further showed the differential roles of the two highly variable regions (Region I spanning amino acid residues 84-89 and Region II spanning amino acid residues 143-148) in substrate binding, catalysis, and sensitivity to the inhibition by 2,6-dichloro-4-nitrophenol. In contrast to the differential sulfotransferase activities of M-form and P-form PSTs toward dopamine and p-nitrophenol, the Dopa/tyrosine sulfotransferase activities were found to be restricted to M-form, but not P-form, PST. Furthermore, the variable Region II of M-form PST appeared to play a predominant role in determining the Dopa/tyrosine sulfotransferase activities of chimeric PSTs. Kinetic studies indicated the role of manganese ions in dramatically enhancing the binding of D-p-tyrosine to wild-type M-form PST. Taken together, these results pinpoint unequivocally the sequence encompassing amino acid residues 84-148 to be the substrate specificity/catalytic domain of both M-form and P-form PSTs and indicate the importance of the variable Regions I and II in determining their distinct enzymatic properties.
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INTRODUCTION |
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Sulfation is a major pathway for the biotransformation/excretion of drugs and xenobiotics as well as endogenous compounds such as catecholamines, cholesterol, steroid and thyroid hormones, and bile acids (1-3). In mammalian cells, the cytosolic sulfotransferases constitute a group of enzymes that catalyze the transfer of a sulfonate group from the active sulfate, PAPS,1 to a substrate compound containing either a hydroxyl or an amino group (4). Two essential components of their catalytic actions therefore are the PAPS binding activity (which is common among various sulfotransferases) and the substrate binding activity (which is unique for individual sulfotransferases). Through sequence comparison, two highly conserved regions (YPKSGTXW close to the N terminus and RKGXXGDWKNXFT near the C terminus) among different cytosolic sulfotransferases had been identified (3). Of these two regions, the latter was shown to be similar to a motif, designated the P-loop, found in the sequences of many ATP- and GTP-binding proteins (5). It was further pointed out (6) that sequences homologous to the C-terminal conserved region are present in PAPS-synthesizing enzymes from Rhizobium meliloti (7) and Escherichia coli (8). Since PAPS is a co-substrate for the sulfation reactions catalyzed by all cytosolic sulfotransferases, it has been speculated that these highly conserved regions may be involved in PAPS binding (3, 5, 6). Some supporting evidence came from an affinity labeling study (9), which showed the labeling of two amino acid residues (lysine 65 and cysteine 66) that are proximal to the conserved region close to the N terminus as mentioned above. With regard to the conserved region near the C terminus, point mutations with alanines substituting for the critical glycine and lysine residues of the P-loop-related sequence in guinea pig estrogen sulfotransferase were shown to result in dramatic decrease in sulfotransferase activity and inability to photoaffinity label with [35S]PAPS (5). Another site-directed mutagenesis study using plant flavonol 3-sulfotransferase also revealed the importance of the invariant arginine residue of the P-loop-related sequence in PAPS binding (10).
Compared with the PAPS binding site, much less information is available regarding the structural determinants for the substrate binding sites of sulfotransferase enzymes. Studies using plant flavonol 3-sulfotransferase and flavonol 4-sulfotransferase, which display distinct substrate and position specificity, have revealed an internal region spanning amino acid residues 92-194 of flavonol 3-sulfotransferase, being responsible for its substrate and position specificity (11). Another study using rat liver hydroxysteroid sulfotransferases as model enzymes showed that the central region spanning amino acid residues 102-164 is essential for the hydroxysteroid sulfotransferase activity (12). Precise structural determinants for the substrate specificity and/or catalytic activity for both these two sulfotransferase models, however, remain largely unknown, except for a recent mutational analysis study suggesting that His-118 of flavonol 3-sulfotransferase might be involved in catalysis (13). More work and better sulfotransferase models are needed to pin down unequivocally the particular regions and specific amino acid residues therein that are responsible for the substrate binding and/or catalytic activities.
In searching for sulfotransferase models for investigating their substrate binding/catalytic sites, our attention has been focusing on human phenol sulfotransferases. Three distinct phenol sulfotransferases have been identified in human tissues (14). One of them is the so called M-form PST that preferentially catalyzes the sulfation of monoamines such as dopamine (15). The other two phenol sulfotransferases, designated the P-form phenol sulfotransferases, preferentially catalyze the sulfation of simple phenolic compounds such as p-nitrophenol (16-18). In addition to their distinct substrate specificity, earlier studies have demonstrated M-form PST to be markedly more thermolabile and 3 orders of magnitude less sensitive to inhibition by 2,6-dichloro-4-nitrophenol (DCNP), a sulfation inhibitor (19). Our recent study also showed that, in contrast to P-form PSTs, M-form PST displayed stereoselective and manganese-dependent Dopa/tyrosine sulfotransferase activities (20). Since M-form and P-form PSTs are greater than 93% identical in their primary sequences (cf. Fig. 1A) and yet display distinct substrate specificity and other properties, these two enzymes serve as an excellent model for investigating the structural determinants for their substrate binding and catalytic activities.
In the present work, a series of chimeric PSTs were constructed by reciprocal exchanges of DNA segments between cDNAs encoding M-form and P-form PSTs. Wild-type and chimeric PSTs thus prepared were expressed in E. coli. Characterization of the recombinant enzymes with respect to substrate specificity and other enzymatic properties allowed for the localization of the domain responsible for their substrate binding/catalytic activities.
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EXPERIMENTAL PROCEDURES |
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Materials--
L-Dopa, D-Dopa,
L-p-tyrosine,
D-p-tyrosine,
DL-m-tyrosine, dopamine,
p-nitrophenol, aprotinin, thrombin, ATP, AMP, SDS, Hepes, Taps, Trizma base (Tris base), dithiothreitol, DCNP, and isopropyl -D-thiogalactopyranoside were products of Sigma.
L-p-Tyrosine-O-sulfate, D-p-tyrosine-O-sulfate,
DL-m-tyrosine-O-sulfate,
dopamine-O-sulfate, and a mixture of L-Dopa
3-O-sulfate and L-Dopa 4-O-sulfate)
were synthesized according to the procedure of Jevons (21). pGEX-2TK gene fusion vector, E. coli BL21 host cells, and
glutathione-Sepharose were products of Pharmacia Biotech Inc.
pBluescript II SK(+) and XL1-Blue MRF' E. coli host strain
were purchased from Stratagene. An I.M.A.G.E. cDNA clone 229827 (GenBankTM Accession number H67938) encoding an isoform of
P-form PST was from Genome Systems, Inc. AmpliTaq DNA
polymerase was from Perkin-Elmer. Long and accurate polymerase chain
reaction (PCR) kit version 2 was from Takara (Shiga, Japan).
Taq dye primer cycle sequencing kits were products of
Applied Biosystems, Inc. T4 DNA polymerase, T4 DNA ligase,
and all restriction endonucleases used were from New England Biolabs.
Sulfate-activating enzymes (ATP sulfurylase and APS kinase) from
Bacillus stearothermophilus were kindly provided by Dr.
Hiroshi Nakajima at Unitika (Uji, Japan). Oligonucleotide primers were
from Operon Technologies, Inc. Carrier-free sodium
[35S]sulfate was from ICN Biomedicals. All other
chemicals were of the highest grades commercially available.
PCR Cloning of Human Liver Phenol Sulfotransferases-- A set of sense (5'-CGCGGATCCATGGAGCTGATCCAGGACACC-3') and antisense (5'-GGCGAATTCTCACAGCTCAGAGCGGAAGCT-3') oligonucleotide primers, based on 5'- and 3'- homologous regions of the reported nucleotide sequences encoding human liver M-form PST (22) and an isoform of P-form PST (23), were synthesized with BamHI and EcoRI restriction sites incorporated at the ends. With these two oligonucleotides as primers, PCR in 100 µl reaction mixtures were carried out under the action of long and accurate Taq DNA polymerase using the human liver M-form PST or P-form PST cDNA as the template. Amplification conditions were 30 cycles of 1 min at 94 °C, 1 min at 55 °C, and 3 min at 72 °C. The final reaction mixtures were applied onto a 0.7% agarose gel, separated by electrophoresis, and visualized by ethidium bromide staining. The discrete 906-base pair PCR products detected were excised from the gel, and the DNA fragments therein were isolated by spin filtration. After BamHI and EcoRI digestion, the PCR products were individually subcloned into the BamHI/EcoRI site of pBluescript II SK(+) and transformed into E. coli XL1-Blue MRF'. To verify their authenticity, the inserts were completely sequenced in both directions using automated cycle sequencing method.
Preparation of Chimeric Constructs-- Plasmids containing chimeric constructs were prepared by reciprocal exchange of internal DNA fragments between wild-type M-form, P-form, or chimeric PSTs. To prepare the first set of chimeric PSTs, wild-type M-form and P-form PST cDNAs were used. Two chimeric PST cDNA clones, designated MPPM and PMMP, were generated by BanI restriction digestion of wild-type M-form and P-form PST sequences, followed by ligation of the middle 281-base pair fragment with the two flanking fragments derived from the opposite wild-type PST sequence. The chimeric PST sequences thus generated as well as wild-type sequences were individually subcloned into the pGEX-2TK prokaryotic expression vector. Construction of the four additional chimeric PST clones (MPMM, MMPM, PMPP, and PPMP) were carried out similarly by reciprocal exchange between the two wild-type (M-PST and P-PST) and two chimeric PSTs (MPPM and PMMP) packaged in pGEX-2TK following Bsu36I digestion. pGEX-2TK plasmids containing, respectively, the two wild-type and six chimeric PST constructs were transformed into E. coli BL21 host cells for the expression of the recombinant wild-type M-form, P-form, and chimeric PST enzymes.
Bacterial Expression and Purification of the Wild-type and
Chimeric PSTs--
Competent E. coli BL21 cells transformed
with pGEX-2TK harboring the cDNA encoding either wild-type or
chimeric PST were grown to A600 nm = ~0.1 in
750 ml of LB medium supplemented with 100 µg/ml ampicillin. Upon
induction with 0.1 mM isopropyl
-D-thiogalactopyranoside overnight at room temperature,
the cells were collected by centrifugation and homogenized in 20 ml of
an ice-cold lysis buffer (containing 10 mM Tris-HCl, pH
8.0, 150 mM NaCl, 1 mM EDTA) using an Aminco French press. The crude homogenate thus prepared was subjected to
centrifugation at 10,000 × g for 30 min at 4 °C.
The supernatant collected was fractionated using 0.5 ml of
glutathione-Sepharose, and the bound fusion protein was treated with 2 ml of a thrombin digestion buffer (containing 5 unit/ml bovine
thrombin, 50 mM Tris-HCl, pH 8.0, 150 mM NaCl,
and 2.5 mM CaCl2). After a 1-h incubation at
room temperature, 5 µg of aprotinin were added to inactivate
thrombin, and the preparation was subjected to centrifugation. The
recombinant enzyme present in the supernatant collected was used for
the enzymatic characterization.
Enzymatic Assay-- The sulfotransferase assays were performed using PAP[35S] as the sulfate donor. The standard assay mixture, with a final volume of 30 µl, contained 50 mM Hepes-NaOH, pH 7.0, 250 mM sucrose, 1 mM dithiothreitol, 14 µM PAP[35S] (15 Ci/mmol), and dopamine (25 µM) or p-nitrophenol (5 µM) as substrate. For assays examining the inhibitory effect of DCNP on the sulfation of dopamine or p-nitrophenol, the assay mixtures were supplemented with different concentrations of DCNP. For the assays using tyrosine or Dopa as substrates, the reaction mixture, with a final volume of 30 µl, contained 50 mM Taps-NaOH, pH 8.75, 250 mM sucrose, 1 mM dithiothreitol, 14 µM PAP[35S] (15 Ci/mmol), and 500 µM L-Dopa or 1 mM D-p-tyrosine or DL-m-tyrosine as substrate. All assay reactions were started by the addition of the purified enzyme preparation, allowed to proceed for 15 min at 37 °C, and terminated by heating at 100 °C for 3 min. The precipitates formed were cleared by centrifugation. The supernatants collected were subjected to the analysis of 35S-sulfated product by either thin-layer chromatography or a two-dimensional thin layer separation procedure previously developed (24).
Miscellaneous Methods-- PAP[35S] (15 Ci/mmol) was synthesized from ATP and [35S]sulfate using ATP sulfurylase and APS kinase from B. stearothermophilus as described previously (25). SDS-polyacrylamide gel electrophoresis was performed on 12% polyacrylamide gels using the method of Laemmli (26). Protein determination was based on the method of Bradford (27) with bovine serum albumin as the standard.
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RESULTS AND DISCUSSION |
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Since the human M-form and P-form PSTs are >93% identical in their primary sequences and yet display distinct substrate specificity and other properties, these two enzymes serve as an excellent model for investigating the structural determinants for the substrate binding specificity as well as the catalytic activity. Examining the aligned amino acid sequences of these two PSTs (22,23), two highly variable regions, designated Regions I and II, were noted (Fig. 1A). It was therefore hypothesized that these two regions may contain the structural determinants for the substrate specificity and/or catalytic activity of these two homologous enzymes. Taking advantage of two BanI restriction sites that are conveniently located close to the 5' and 3' side of the nucleotide sequences encoding Regions I and II (Fig. 1B), we decided to first prepare two chimeric cDNAs, designated MPPM and PMMP (Fig. 1B). To further narrow down the differential importance of Regions I and II, four more chimeric PST clones were prepared by making use of the Bsu36I restriction site located between Regions I and II (Fig. 1B). The recombinant chimeric PSTs as well as wild-type M-form and P-form PSTs were expressed for functional characterization.
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Construction and Expression of Chimeric Phenol Sulfotransferases-- Based on the procedure described under "Experimental Procedures," wild-type M-form and P-form PST cDNAs and chimeric PST cDNAs constructed were individually subcloned into the pGEX-2TK prokaryotic expression vector and transformed into E. coli host cells for expressing the recombinant enzymes. As shown in Fig. 2, the recombinant wild-type and chimeric PSTs fractionated from the homogenates of transformed E. coli cells using glutathione-Sepharose and cleaved off the fusion proteins by thrombin appeared to be highly homogeneous upon SDS-polyacrylamide gel electrophoresis. It was noted that there were some differences in electrophoretic mobility among M-form and P-form PSTs as well as chimeric PSTs despite the similar predicted molecular masses of these enzymes (cf. Fig. 1A). While the reasons are not clear, this aberrant phenomenon has previously been documented (17). It is to be pointed out that human M-form and P-form PSTs have also been expressed previously in either E. coli or COS cells (17, 22, 29-31).
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Sulfotransferase Activities of Recombinant Wild-type M-form, P-form, and Chimeric PSTs toward Dopamine and p-Nitrophenol-- To examine whether the sequence defined by the variable Regions I and II is indeed responsible for the substrate specificity, the recombinant wild-type and chimeric PSTs were subjected to sulfotransferase assays using dopamine (a typical substrate for M-form PST activities) and p-nitrophenol (a typical substrate for P-form PST activities). As shown in Table I, wild-type M-form and P-form PSTs, as expected, displayed distinct substrate specificities, with the former having higher Vmax/Km for dopamine and the latter having higher Vmax/Km for p-nitrophenol. Interestingly, when the regions coded by the 281-base pair BanI fragments (cf. Fig. 1) were reciprocally exchanged, the substrate phenotypes of the resulting chimeric PSTs (MPPM and PMMP) switched to the ones originally displayed by the opposite PSTs. The Vmax/Km values of PMMP for dopamine or p-nitrophenol were surprisingly close to those of the wild-type M-form PST. The Vmax/Km values of MPPM for dopamine or p-nitrophenol, although showing some variations, were also close to those of the wild-type P-form PST. Based on these results, it can be concluded that the internal region coded by the 281-base pair BanI fragment indeed contains the structural determinants for the substrate specificity and, to a large extent, the catalytic activity of either M-form or P-form PST. This is also supported by the data on the Dopa/tyrosine sulfotransferase activities (cf. Table II). For chimeric PSTs (MPMM, MMPM, PMPP, and PPMP) that have been subjected to reciprocal exchanges of shorter sequences encompassing either Region I or Region II, the results were also informative, although somewhat more complicated. Their kinetic constants compiled in Table I indicate the importance of both Regions I and II in maintaining the substrate specificity of M-form and P-form PSTs. For MPMM and MMPM with either Region I or II exchanged with that of P-form PST, dramatic decrease in Vmax/Km and increase in Km were found with dopamine as substrate. These results indicate that both Regions I and II of M-form PST are needed for the efficient binding of dopamine, a positively charged compound at physiological pH. With p-nitrophenol as substrate, although the Km of MPMM remained in the same order of magnitude, the Km of MMPM decreased more than eight times. This latter finding indicates that the Region II sequence of the P-form PST may encompass amino acid residue(s) favoring the binding of p-nitrophenol, an uncharged compound. For PMPP and PPMP with either Region I or Region II exchanged with that of M-form PST, the changes in Km with p-nitrophenol as substrate were much greater than those with dopamine as substrate. Furthermore, a dramatic decrease in Vmax/Km with p-nitrophenol as substrate was observed. These results may suggest that the incorporation of Region I or II sequence derived from M-form PST, which are relatively more enriched in charged amino acid residues (cf. Fig. 1A), may hamper the binding of the uncharged p-nitrophenol. For all eight wild-type M-form, P-form, and chimeric PSTs, it was noted that the ones that contain Region I sequence derived from M-form PST displayed markedly higher Vmax values than those containing Region I sequence derived from P-form PST. These results imply that the catalytic amino acid residues present in Region I sequence of M-form PST may be intrinsically more efficient than those present in the Region I sequence of P-form PST. Taken collectively, the kinetic constant data compiled in Table I provide ample evidence indicating that the sequence coded by the 281-base pair BanI fragments (cf. Fig. 1) contains the structural determinants for both the substrate binding and catalytic activities. Excluding the identical or homologous amino acid residues present within this sequence, it can be further postulated that the variable Regions I and II may in fact account for the distinct substrate binding and catalytic activities between M-form and P-form PSTs. It is interesting to note that analysis of aligned amino acid sequences has revealed a low degree of homology in the central region among cytosolic sulfotransferases (3, 32). It is possible, therefore, that the central region may also constitute the substrate specificity domain for other sulfotransferase enzymes. The results presented above indeed provide support for this hypothesis.
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DCNP Inhibition of Wild-type M-form, P-form, and Chimeric PSTs-- Sensitivity to the inhibition by DCNP, a sulfotransferase inhibitor (19), has been widely used to distinguish M-form and P-form PSTs present in a variety of human tissues (33, 34). We were interested in investigating the structural basis for the differential sensitivity of these two highly homologous sulfotransferases to DCNP. As shown in Fig. 3, similar to the results previously reported (33, 34), recombinant wild-type P-form PST was approximately 3 orders of magnitude more sensitive to the inhibition by DCNP than wild-type M-form PST. For the two chimeric PSTs (MPPM and PMMP) with the regions coded by the 281-base pair BanI fragments (cf. Fig. 1) reciprocally exchanged, the DCNP inhibition phenotype changed to that of the opposite PST. For chimeric PSTs (MPMM, MMPM, PMPP, and PPMP) that have been subjected to reciprocal exchange of a shorter sequence encompassing either Region I or Region II, the sensitivities lie in between those of the M-form and P-form PSTs. Their IC50 values, however, were closer to those of the parental sources of the newly incorporated sequences that contained Region II. These results collectively indicate that for both M-form and P-form PSTs, the sequence defined by the variable Regions I and II (cf. Fig. 1) also contains the structural determinants for the sensitivity to the inhibition by DCNP. Moreover, of the two variable regions, Region II appeared to play a more important role in determining this property.
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Dopa and Tyrosine Sulfation-- In a recent study (20), we have demonstrated that M-form PST exhibited unique Dopa/tyrosine sulfotransferase activities. This finding led us to hypothesize that M-form PST, among other physiological involvements, may also contribute to the homeostatic regulation of Dopa and tyrosine by converting the excess of these compounds to the more water-soluble sulfated forms, thereby facilitating their removal from the body. Since Dopa and tyrosine serve as precursors for the biosynthesis of catecholamines (including dopamine, norepinephrine, and epinephrine), the M-form PST may be important in vivo for the normal functioning of the nervous system and/or the endocrine system. To investigate the structural determinants for the Dopa/tyrosine sulfotransferase activities of M-form PST, we decided to characterize the enzymatic activities of the wild-type M-form, P-form, and chimeric PSTs. As shown in Table II, in contrast to the differential activities of M-form and P-form PSTs toward dopamine or p-nitrophenol, only M-form, but not P-form, PST could catalyze the sulfation of Dopa and tyrosine isomers. When the regions coded by the 281-base pair BanI fragments (cf. Fig. 1) were reciprocally exchanged, the chimeric PMMP, but not MPPM, displayed the similar Dopa/tyrosine sulfotransferase activities. These results provided the most clear-cut evidence indicating that the sequence defined by the variable Regions I and II in M-form PST contains the structural determinants for the substrate specificity for Dopa and tyrosine. Of the other four chimeric PSTs, MPMM and PPMP, but not MMPM and PMPP, displayed lower, yet comparable, Dopa/tyrosine sulfotransferase activities for L-Dopa, D-Dopa, and DL-m-tyrosine. No sulfotransferase activities were detected for MPMM and PPMP with D-p-tyrosine or L-p-tyrosine as substrate. Although it is possible that their activities might have been below the detection limit of the analytical method used, the results may indicate that the presence of the M-form PST Region II sequence alone may allow only the sulfation of m- hydroxyl group, but not p- hydroxyl group. A combination of the M-form PST Regions I and II may be required for the sulfation of p- hydroxyl group. In another study, we have demonstrated unequivocally the sulfation of Dopa, as catalyzed by M-form PST, being exclusively on the m- hydroxyl group.2 Taken together, the results compiled in Table II indicate that variable Region II of M-form PST plays a predominant role in the sulfotransferase activities for Dopa and tyrosine, although variable Region I may be needed for the sulfotransferase activity for L- or D-p-tyrosine.
Effects of Manganese Ions on the Kinetic Constants of M-form PST-- In an earlier study (35), we observed a remarkable stimulation of the Dopa/tyrosine sulfotransferase activities present in HepG2 human hepatoma cells by manganese ions. Although the physiological relevance of this manganese dependence of Dopa/tyrosine sulfotransferase activity remains unclear, it was speculated that there may be a link to the manganese poisoning previously reported to occur frequently among miners (36). The underlying hypothesis is that, for those afflicted with manganese poisoning, the Dopa/tyrosine sulfotransferase may be activated in vivo. Increasing amounts of L-Dopa, L-p-tyrosine, and L-m-tyrosine, which serve directly or indirectly as biosynthetic precursors for catecholamines (37), may become sulfated and excreted. The lowered levels of these Dopa and tyrosine isomers in turn may result in decreased production of dopamine and other catecholamines. Indeed, individuals afflicted with manganese poisoning have been reported to display symptoms resembling those of Parkinson's disease (38), and reduction or even elimination of some of these symptoms has been observed in those receiving treatment with L-Dopa (28, 38). In a recent study (20), we have demonstrated unequivocally that the manganese-dependent Dopa/tyrosine sulfotransferase activities found in HepG2 cells in fact are associated with M-form PST. The stimulatory effect of manganese ions on M-form PST was found to be the strongest with D-p-tyrosine as substrate. To investigate the mechanistic basis of the manganese stimulation of the Dopa/tyrosine sulfotransferase activities of M-form PST, we therefore decided to examine the kinetics of the sulfation reaction with D-p-tyrosine as substrate. As shown in Fig. 4, the Lineweaver-Burk double reciprocal plot derived from the data obtained indicate that, although the Vmax of the sulfation of D-p-tyrosine in the presence or absence of manganese ions remained in the same order of magnitude, the Km values appeared to be dramatically different. The calculated Vmax, Km, and Vmax/Km values under these two assay conditions are compiled in Table III. Based on these results, it can be concluded that the presence of manganese ions greatly enhanced the binding of D-p-tyrosine and the catalytic efficiency (by more than 260-fold as reflected by Vmax/Km) of M-form PST. Exactly how this is achieved at the molecular level, however, remains to be elucidated.
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ACKNOWLEDGEMENTS |
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We thank Junko Katafuchi and Ken Yanagisawa for technical assistance and Dr. Hiroshi Nakajima for kindly providing us with sulfate-activating enzymes.
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FOOTNOTES |
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* This work was supported in part by Grant-in-aid 95G-564 from the American Heart Association, Texas affiliate, a joint research grant from the Monbusho International Scientific Research Program, Japan (to M. S.), and a Japan Society for the Promotion of Science (JSPS) post-doctoral fellowship for research abroad (to Y. S.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of
Biochemistry, The University of Texas Health Center, P. O. Box 2003, Tyler, TX 75710. Tel: 903-877-2862; Fax: 903-877-2863; E-mail: liu{at}uthct.edu.
1 The abbreviations used are: PAPS, adenosine 3'-phosphate 5'-phosphosulfate; PST, phenol sulfotransferase; Dopa, 3,4-dihydroxyphenylalanine; DCNP, 2,6-dichloro-4-nitrophenol; Taps, 3-[tris(hydroxymethyl)methyl]aminopropanesulfonic acid; PCR, polymerase chain reaction; APS, adenosine 5'-phosphosulfate.
2 M. Suiko, Y. Sakakibara, H. Sakaida, H. Yoshikawa, and M.-C. Liu, unpublished results.
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REFERENCES |
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