Coagulation Factor XIIIa Undergoes a Conformational Change Evoked by Glutamine Substrate
STUDIES ON KINETICS OF INHIBITION AND BINDING OF XIIIa BY A CROSS-REACTING ANTIFIBRINOGEN ANTIBODY*

Olga V. MitkevichDagger , John R. Shainoff§, Patricia M. DiBello, Vivien C. Yee, David C. Teller, Gary B. Smejkal, Paul D. Bishopparallel , Irina S. KolotushkinaDagger , Karl Fickenscher**, and Gennady P. SamokhinDagger Dagger

From the Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, Dagger  Institute of Experimental Cardiology, Russian Cardiology Research Center, Moscow 121552, Russia,  Department of Biochemistry, University of Washington, Seattle, Washington 98195, parallel  ZymoGenetics Inc., Seattle, Washington 98102, ** Behring Diagnostics GmbH, Marburg, Germany D-35001, and Dagger Dagger  Department of Medicine, East Carolina University School of Medicine, Greenville, North Carolina 27853

    ABSTRACT
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Abstract
Introduction
Materials & Methods
Results
Discussion
References

Coagulation factor XIIIa, plasma transglutaminase (endo-gamma -glutamine:epsilon -lysine transferase EC 2.3.2.13) catalyzes isopeptide bond formation between glutamine and lysine residues and rapidly cross-links fibrin clots. A monoclonal antibody (5A2) directed to a fibrinogen Aalpha -chain segment 529-539 was previously observed from analysis of end-stage plasma clots to block fibrin alpha -chain cross-linking. This prompted the study of its effect on nonfibrinogen substrates, with the prospect that 5A2 was inhibiting XIIIa directly. It inhibited XIIIa-catalyzed incorporation of the amine donor substrate dansylcadaverine into the glutamine acceptor dimethylcasein in an uncompetitive manner with respect to dimethylcasein utilization and competitively with respect to dansylcadaverine. Uncompetitive inhibition was also observed with the synthetic glutamine substrate, LGPGQSKVIG. Theoretically, uncompetitive inhibition arises from preferential interaction of the inhibitor with the enzyme-substrate complex but is also found to inhibit gamma -chain cross-linking. The conjunction of the uncompetitive and competitive modes of inhibition indicates in theory that this bireactant system involves an ordered reaction in which docking of the glutamine substrate precedes the amine exchange. The presence of substrate enhanced binding of 5A2 to XIIIa, an interaction deemed to occur through a C-terminal segment of the XIIIa A-chain (643-658, GSDMTVTVQFTNPLKE), 55% of which comprises sequences occurring in the fibrinogen epitope Aalpha -(529-540) (GSESGIFTNTKE). Removal of the C-terminal domain from XIIIa abolishes the inhibitory effect of 5A2 on activity. Crystallographic studies on recombinant XIIIa place the segment 643-658 in the region of the groove through which glutamine substrates access the active site and have predicted that for catalysis, a conformational change may accompany glutamine-substrate binding. The uncompetitive inhibition and the substrate-dependent binding of 5A2 provide evidence for the conformational change.

    INTRODUCTION
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Abstract
Introduction
Materials & Methods
Results
Discussion
References

Factor XIII (fXIII)1 is a member of the class of enzymes known as transglutaminases, which catalyze displacement of amide ammonia at the gamma -position in glutamine residues by replacing it with another amine, usually an epsilon -amino group from a suitable lysine residue (1-4). Formation of epsilon -(gamma -glutamyl)lysine isopeptide bonds functions in both intra- and intermolecular cross-linking of proteins. Among the many functions of fXIII, the best known are 1) the cross-linking of fibrin, which stabilizes clots against redissolution by fibrinogen (5) and plasmin (6), 2) the linking of alpha 2-plasmin inhibitor to fibrin for further protection from plasmin (7, 8), and 3) the cross-linking of matrix proteins, among them, fibronectin, collagen, and thrombospondin, which facilitate wound healing (9). Platelet and placental fXIII are homodimers composed of two identical A chains, but plasma fXIII is a heterotetramer composed of two A and two B chains (10). Plasma fXIII is activated by two steps: 1) thrombin cleavage of a 4 kDa fragment from the N terminus of the A-chains and 2) Ca-dependent dissociation of the two B chains from the tetramer, yielding functionally active A-chain dimers (XIIIa) (11, 12). FXIII was discovered in 1948 (13), and since that time has been extensively studied. Its primary structure and its three-dimensional structure including its active site are all known (14-17). But still unknown are the determinants of substrate docking and specificity (18). Many of the domains are highly conserved and do not yield antibodies to probe their function. In this study, we describe multiple types of inhibition of XIIIa produced with a cross-reacting monoclonal antibody (5A2) directed to fibrinogen Aalpha -chains in the region of residues 529-539. The modes of inhibition change from uncompetitive to competitive depending on which substrate (amine donor or glutamine acceptor) is examined and these changes give insight into the order of substrate usage. Enhanced binding of 5A2 to XIIIa in the presence of substrate is viewed as evidence that substrate binding induces conformational changes in XIIIa. These observations support earlier inferences on the catalytic mechanism gained from crystallographic studies on recombinant fXIII A-chains (rXIIIA).

    MATERIALS AND METHODS
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Abstract
Introduction
Materials & Methods
Results
Discussion
References

Proteins and Reagents-- Initial studies on inhibitory effects of 5A2 were carried out directly in plasma. Kinetic studies used ZymoGenetics Inc. (Seattle, WA) (rXIIIA) (19). Synthetic substrates for XIIIa were used as supplied in Berichrom® XIIIa assay kits except when supplemented with the glutamine substrate LGPGQSKVIG from Behring Werke (Marburg, Germany). Bovine N,N-dimethylated casein, dansylcadaverine, phenylmethylsulfonyl fluoride, trypsin, dithiothreitol, Aprotinin®, and guinea pig liver tissue transglutaminase were obtained from Sigma. Bovine thrombin was from U. S. Biochemical Corp. Hirudin was from Pentapharm AG (Basel). Phenylprolylarginine chloromethyl ketone was from Chemica Alta Ltd (Edmonton, Alberta, Canada). Antifibrinogen monoclonal antibodies (mAb) 5A2 and 7F8 were made as described previously (20, 21), as was the anti-FPA (22). Anti-angiotensin-converting enzyme mAb 9B9 (23) was generously provided by Dr. S. M. Danilov (Russian Cardiology Research Center, Moscow). Rabbit anti-XIIIa antibodies were generated using rXIIIA as antigen and purified by elution (0.35 M citrate, pH 3.5) from protein A-Sepharose (Amersham Pharmacia Biotech). Except where indicated, all protein solutions were in Tris-buffered saline containing 0.1 mM EDTA (TBSE) at pH 7.4.

Human fibrinogen was purified from the donor plasma by sequential precipitations with ethanol, glycine, and ammonium sulfate/epsilon -amino caproic acid as described (24). Where indicated, it was immunochemically freed of fXIII by passing it (36 mg, 3 ml) through a 10-ml column of affinity-purified rabbit anti-XIIIa antibody (20 mg) that was linked to beaded 2% glyoxyl-agarose gel (Global Imports, Tampa, FL) essentially as described (25). The columns were reused after a flush with 5 ml of 4 M guanidine-HCl and re-equilibrated in TBSE after temporarily resuspending the gel in 10 ml of TBSE containing enzyme inhibitors (0.2 mM phenylmethylsulfonyl fluoride, 0.1 mg/ml Aprotinin®, and 0.1 mM phenylprolylarginine chloromethyl ketone).

Effect of 5A2 on Cross-linking of Fibrinogen-- These studies simply involved adding calcium (5 mM) and dithiothreitol (2.5 mM) in the presence and absence of added 5A2 (4 mg/ml) to the fibrinogen (2 mg/ml) preparation before removing the contaminating XIIIa from the fibrinogen. The observed cross-linking was verified as due to the presence of active XIIIa in the fibrinogen preparation, because it occurred without thrombin activation and in presence of hirudin, it did not change during a parallel incubation (6 h, 22 °C) with 8 mg/ml rXIIIA added, and it did not occur at all after passing the fibrinogen through the anti-fXIII affinity gel.

Cross-linked products were separated by SDS-polyacrylamide gel electrophoresis of thiol-reduced specimens and identified after Coomassie staining by comparison with previous electropherograms identifying the polypeptide chain compositions of the products (26).

Activation of rXIIIA-- To preactivate the rXIIIA (2 mg/ml), it was incubated (15 min, 37 °C) with thrombin (0.5 units/ml) at pH 7.4 in the presence of CaCl2 (2 mM) and a trace amount of fibrinogen (2 mg/ml). The activation was then terminated, and thrombin was inactivated with hirudin (10 units/ml). Activation of rXIIIA that was precoated on microtiter wells was performed with 0.07 units of thrombin with 5 mM calcium for 30 min at 37 °C, and the thrombin was subsequently inactivated with 0.3 units of hirudin before washing and blocking for enzyme-linked immunosorbent assay measurements.

Assays of XIIIa Activities Using Dimethylcasein As the Glutamine Substrate and Dansylcadaverine As the Amine Substrate-- These assays followed continuous changes in fluorescence arising from the incorporation of dansylcadaverine into casein by the method of Lorand et al. (27). The fluorescence was measured in 96-well microplates using a Perkin-Elmer luminescence spectrometer LS50, with excitation elicited at lambda ex = 360 nm and emission followed at lambda em = 510 nm. The buffer consisted of 0.1 M sodium borate containing 10 mM CaCl2 and 5 mM dithiothreitol at pH 9.0. The concentrations of preactivated XIIIa in most of the experiments was 0.03 mg/ml. Dimethylcasein concentrations were varied from 0.06 to 0.38 mg/ml in the presence of a saturating concentration of dansylcadaverine (200 mM), and dansylcadaverine concentrations were varied 1-5 mM in the presence of a saturating concentration of dimethylcasein (5 mg/ml). Reaction rates were assessed during initial stages when fluorescence varied linearly with time.

Measurements of XIIIa Activity Using Berichrom® fXIII Synthetic Substrates-- This assay uses a synthetic decapeptide (LGPGQSKVIG) as glutamine substrate and glycine ethyl ester as the amine substrate and measures transglutaminase activity from the rate of ammonia release from the peptide using an auxiliary enzyme system (28). The ammonia release is followed colorimetrically from consumption of NADH as the ammonia is driven into reaction with alpha -ketoglutarate by glutamate dehydrogenase, all of these components apart from NADH being packaged in the "reporting" solution of the Berichrom® fXIII kit. The assays were carried out according to the recommended procedure of the supplier except for two modifications made to characterize the kinetics of 5A2 inhibition in relation to LGPGQSKVIG concentrations. We substituted rXIIIA/5A2 mixtures for the plasma specified for measuring plasma fXIII in the kit. Secondly, since the reporting solution contained a saturating level of LGPGQSKVIG, we diluted it in half and varied the concentration of LGPGQSKVIG by supplementing the diluted solution with added peptide. As determined with the peptide reconstituted to its original level, apparent reaction velocities, both in the presence and absence of 5A2 reported with the diluted solutions, were consistently one-half those reported with the undiluted solution due to the lower concentration of reporting enzyme (glutamate dehydrogenase) in the diluted system.

Preparation of C-terminal-truncated 51-kDa Fragment of rXIIIA-- This tryptic derivative of rXIIIA was prepared essentially as described by Greenberg et al. (29). The digest contained rXIIIA (2 mg/ml), trypsin (1 mg/ml), and CaCl2 (10 mM) at 37 °C. SDS-polyacrylamide gel electrophoresis showed that full conversion of rXIIIA to the 51-kDa fragment required 2.5 h of incubation under these conditions. The digestion was terminated by adding Aprotinin® (0.14 mg/ml). The electropherograms showed that none of the 80-kDa parent rXIIIA remained unconverted. A shorter incubation of 1.5 h left 75% converted. The 51-kDa fragment precipitated out of solution during the incubation, was centrifuged, and resuspended in TBSE, and the suspension was divided into 0.1-ml aliquots, which were stored frozen. Just before assay, the suspension was thawed, and admixed with an equal volume of 6 M urea (3 M final) to dissolve the protein immediately before measuring its activity.

Enzyme-linked Immunosorbent Assay for Binding-- Nunc immuno plates (Vanguard Intl., Neptune, NJ) were coated with rXIIIA in TBS (10 µg/ml, 100 µl/well, 18 h, 4 °C), washed with TBS, and blocked with 3% bovine serum albumin in TBS containing 0.1% Tween-20 (TBSTw). Primary antibodies, either 5A2 or control anti-FPA mAbs (100 µg/ml, 100 µl/well), were added in 0.1% bovine serum albumin in TBSTw supplemented with either Behrichrom substrate or blank (10 µl/well). After 18 h of equilibration at 4 °C followed by a TBSTw wash, the retained 1° antibody was fixed in place with cold 0.25% glutaraldehyde in phosphate-buffered saline (100 µl/well, 4 °C, 10 min), and the plates were washed (TBSTw) and quenched 0.1% bovine serum albumin in TBSTw (200 µl/well, 37 °C, 10 min). After further washing, secondary reporter antibody (horseradish peroxidase-goat-anti-mouse Ig at 1:1000 dilution, 100 µl/well) was added, and the excess was washed away after 2 h at 23 °C. Retained 2° antibody was assessed from peroxidase activity toward orthophenylenediamine.

    RESULTS
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Abstract
Introduction
Materials & Methods
Results
Discussion
References

Inhibitory Effects of mAb 5A2 on Fibrinogen alpha  and gamma -Chain Cross-linking-- In the course of studies on the effect of 5A2 on the cross-linking of fibrinogen we observed (Fig. 1) that gamma gamma -dimer formation was inhibited to a degree comparable to the subsequent (26) incorporation of fibrinogen alpha -chains2into hybrid alpha gamma gamma -trimers. The inhibition of gamma -chain cross-linking was not detected in our preceding study, which characterized only the effect of 5A2 on end-stage plasma clots (20). The inhibition of gamma -chain cross-linking together with searches indicating that there was no homology between the 5A2 epitope on fibrinogen (20) and the amino acid sequences in the fibrinogen gamma -chains, prompted us to investigate whether the inhibitory effect of 5A2 was arising from a direct interaction with XIIIa. We then proceeded to examine the effects of 5A2 on substrates other than fibrinogen.


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Fig. 1.   Electropherograms illustrating the inhibitory effect of 5A2 on both gamma - and alpha -chain cross-linking of fibrinogen by XIIIa. The first lane (at left) shows the noncross-linked alpha , beta , and gamma  chains in the parent fibrinogen stored in 0.1 mM EDTA. Cross-linking initiated by adding calcium and dithiothreitol in the absence of 5A2 (center lane) caused a nearly complete incorporation of gamma -chains into characteristic gamma gamma -dimers and subsequent mixed alpha gamma gamma oligomers with accompanying substantial disappearance of noncross-linked alpha -chains. In the presence of 5A2 (right lane), the cross-linking of both gamma - and alpha -chains was appreciably arrested, as evidenced by the diminished disappearance of noncross-linked chains and reductions in the levels of both gamma gamma -dimers and alpha gamma gamma -trimers, the percentage change in gamma - and alpha -chains products being of comparable magnitudes. The restoration of noncross-linked gamma -chains appears greater than would be expected from the reduced levels of cross-linked products because 5A2 heavy chains co-migrated in that band. The light chains from added 5A2 appear at the bottom of the lane.

Kinetics of Dansylcadaverine Incorporation into Dimethylcasein-- This reaction was inhibited by 5A2 in a concentration-dependent manner and was investigated further in relation to the utilization of the dimethylcasein (Fig. 2) and the dansylcadaverine (Fig. 3) at varying concentrations. When dimethylcasein concentrations were varied, we obtained parallel, nonintersecting Lineweaver-Burk plots of 1/upsilon versus 1/[dimethlycasein] at 5A2 concentrations ranging to 0.6 mg/ml (Fig. 2). A replot of the 1/upsilon intercepts versus the 5A2 concentrations was linear, with a negative intercept at 5A2 concentrations corresponding to a Ki of 0.8 mM (Fig. 2, inset). The parallel Lineweaver-Burk plots and linear replot conformed with a pattern of inhibition termed "uncompetitive," where the inhibitor is directed principally toward the enzyme-substrate complex (30), the glutamine substrate in this instance. These effects were specifically caused by 5A2 because no inhibition was observed with 7F8 and were also specifically directed to XIIIa because no inhibitory effects of 5A2 were observed toward transamination of these substrates by tissue transglutaminase (negative results not shown).


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Fig. 2.   Lineweaver-Burk plots characterizing 5A2 as an uncompetitive type of inhibitor of the utilization of glutamine substrate (dimethylcasein) by rXIIIA. Reaction velocities (upsilon ) were determined from initial rates of increase in fluorescence arising (27) from incorporation of the dansylcadaverine at fixed concentration into dimethylcasein at varying concentrations in the presence and absence of 5A2. The presence of 5A2 diminished reaction velocities (indicated by the upward shifts in the intercepts on the 1/upsilon axis where 1/upsilon  = 1/Vmaxi,) but did not alter the slopes, a pattern typical of uncompetitive inhibition. The inset shows the linear relationship between the 1/upsilon -axis intercepts versus the 5A2 concentrations, which extrapolate to -0.8 µM for the value of -Ki.


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Fig. 3.   Lineweaver-Burk plots characterizing 5A2 as a competitive type of inhibitor of the utilization of amine substrate (dansylcadaverine) by rXIIIA. Reaction velocities (upsilon ) were determined as for Fig. 3, but the concentration of dimethylcasein was fixed at a high level, and concentrations of dansylcadaverine were varied in the presence and absence of 5A2. Here, the presence of 5A2 shifted the slopes rather than the 1/upsilon axis intercepts, a pattern typical of competitive inhibition. The inset shows the linear relationship between the slopes versus the 5A2 concentrations, which extrapolate to -0.4 µM for the value of -Ki.

The Lineweaver-Burk plots of 1/upsilon versus 1/[dansylcadaverine], on the other hand, yielded slopes that varied with the 5A2 concentrations (Fig. 3) and conformed with a competitive mode of inhibition. A linear replot of the slopes versus 5A2 concentrations intercepted the negative 5A2 axis at Ki = 0.4 µM (Fig. 3, inset).

Transamination of Berichrom® Decapeptide LGPGQSKVIG with Glycine Ethyl Ester-- As illustrated (Fig. 4), 5A2 exhibited a concentration-dependent inhibition of XIIIa-catalyzed release of ammonia from the decapeptide in a pattern consistent with the uncompetitive mode of inhibition observed with the other nonfibrinogen-related glutamine substrate, dimethylcasein. The 1/upsilon intercepts (corresponding to values for 1/Vmax) at 1/S = 0 on the Lineweaver-Burk plots varied linearly with 5A2 concentrations (Fig. 4, inset). Extrapolating this replot to the base-line 1/Vmax = 0 yielded an estimate of 0.9 µM for the value of Ki, which could be off by a factor of two (95% confidence interval) because of the length of the extrapolation.


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Fig. 4.   Effect of 5A2 on transglutaminase activity of rXIIIA toward Berichrom® fXIII synthetic substrates (recorded from changes in absorbance (l = 340 nm) due to the color reaction of ammonia released from LGPGQSKVIG by glycine ethyl ester in the presence of varying concentrations of 5A2). Reaction velocities (upsilon ) were calculated from linear least square slopes of the plots. Inset, replot of the reciprocal slopes (1/upsilon ) versus the concentrations of inhibitor (5A2). The intercept at -0.9 µM 5A2 represents the value of -Ki.

Homology Searches-- The inhibitory effects of 5A2 on utilization of nonfibrinogen substrates and the absence of effect on tissue transglutaminase suggested that 5A2 was interacting with a segment of XIIIa with a sequence similar to that of the fibrinogen epitope. The fibrinogen epitope was identified from CNBr and tryptic fragments to be located in the region of Aalpha 529-539, the only fragment from the combined digests that substantially inhibited (18%) binding of 5A2 to fibrinogen Aalpha -chains (20). Searches for similarities between the sequence of this peptide and the amino acid sequences (16, 31) in XIIIA were made using both the PROPHET (Bolt Beranek and Newman, Inc., Cambridge MA) and the BLAST network service (NCBI). Both searches found a segment near the C terminus of the Aalpha -chain 643-658 of XIIIA that consists in part (55%) of sequences occurring in the region of the fibrinogen Aalpha -chain epitope, and no other regions were found (>3% homology) in either XIIIA, fibrinogen, or casein. XIIIA: 643GSDMTVTVQFTNPLKE; Fgn Aalpha : 529GS---GSGIFTN-TKE540.

No Effect on an Enzymically Truncated XIIIA-- To test the importance of the C-terminal domain for the inhibitory effects of 5A2, we examined its effect on cross-linking of a tryptic fragment of 5A2 lacking the putative cross-reacting domain. Greenberg et al. (29) observed that a tryptic fragment of XIIIA comprising residues Gly38-Lys513 retained 20% of its initial activity. On trypsinizing rXIIIA we obtained a pure 51-kDa fragment that precipitated during the digestion. The fragment exhibited transglutaminase activity in the Berichrom® fXIII assay when it was admixed (0.015 mg/ml) with the substrates immediately after dissolving it in 3 M urea. Unlike intact XIIIa, activity of the fragment was unaffected by 5A2 (Fig. 5). This result supported the hypothesis that 5A2 was inhibiting intact XIIIa by interacting with the segment in the XIIIa C-terminal domain, residues 643-658 with sequence similar to the 5A2 epitope.


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Fig. 5.   Comparison of effects of 5A2 on activities of rXIIIA and the trypsin-truncated rXIIIA using the Berichrom® fXIII assay kit.

Binding of 5A2 with rXIIIA-- Attempts to detect binding of 5A2 to plated rXIIIA by enzyme-linked immunosorbent assay methods failed until we resorted to fixing the initially bound 5A2 with glutaraldehyde to prevent dissociative losses during exposure (30 min) to secondary, reporter antibody. This approach detected binding of 5A2 to the rXIIIA zymogen substantially above that of a control mAb, anti-FPA (Fig. 6). Activating the rXIIIA with thrombin promoted binding of both the 5A2 and anti-FPA mAbs (5A2 more so), whether the activation was performed before plating the rXIIIA (columns labeled XIIIa) or after plating (columns labeled XIIIA*). We suspect that the enhanced binding of the anti-FPA was due to an effect of thrombin, because plating thrombin caused some retention of the anti-FPA not observed with 5A2. Unlike the binding of anti-FPA, the 5A2 binding became substantially enhanced further when the synthetic substrate from the Berichrom® fXIII kit was added to the medium during incubation. The added substrate also caused a slight increase in binding to the zymogen, which could conceivably be due to the normal development of low levels of activity by zymogen without thrombin treatment. Essentially the same enhancing effects of added substrate were reproducibly obtained in repeated experiments. The enhanced binding of 5A2 after activation with thrombin and the further enhancement after adding substrate were viewed as indications that substrate binding was inducing a conformational change leading to greater exposure of the epitope for 5A2 binding.


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Fig. 6.   Effect of adding (+) or omitting (-) the Berichrom® substrate on the binding of 5A2 and control (anti-FPA) mAbs to rXIIIA plated either 1) without activation (XIIIA), 2) preactivated with thrombin (XIIIa), or 3) without pre-activation but activated with thrombin after plating (XIIIA*). ELISA, enzyme-linked immunosorbent assay.

    DISCUSSION
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Abstract
Introduction
Materials & Methods
Results
Discussion
References

The C-terminal domain of XIIIA had been shown by Greenberg et al. (29) to have some influence on the transglutaminase activity of the enzyme, as judged by the substantial (80%) loss of activity after removing the domain with trypsin. Attempts to probe its function immunochemically have proven difficult, because it is poorly immunogenic (29). As described here, we serendipitously found that an antibody (5A2) directed to a C-terminal domain of the fibrinogen Aalpha -chain (529-539) inhibits XIIIa in a manner dependent on the C-terminal domain of the enzyme. This inhibition is deemed to involve a cross-reactivity with residues 643-658 in the XIIIA chain, which share homology with the fibrinogen epitope, and further appears to be partially dependent on interaction with XIIIa complexed with substrate. The modes of inhibition have important implications for the structure and activity of XIIIa.

The crystallographic structure of XIIIA was recently elucidated (17, 32, 33). As illustrated (Fig. 7), the cross-reacting segment 643-658 forms a strand in the outer beta  sheet of the C terminus of the barrel 2 domain of XIIIA. The lysine 657 residue is located at the gap between the barrel 1 and barrel 2 domains, which has been identified as one of two possible loci through which glutamine substrates gain access to the catalytic site of the enzyme (32). The inhibition of utilization of glutamine substrates (both the dimethylcasein and the synthetic peptide LGPGQSKVIG) was uncompetitive, an indication (30) that the principal mode of that inhibition was directed to the enzyme-glutamine complex. Any other mode of enzyme-inhibitor interaction is incidental. In essence, the glutamine binding acts as an activator of the catalytic conformational change that enables utilization of the amine substrate. Utilization of the amine substrate was inhibited competitively, probably not because 5A2 acts as an analog of the amine substrate, but, more likely, because the amine substrate cannot bind to the ternary glutamine donor·XIIIa·5A2 complex. The independent observation of increased 5A2 binding to XIIIa in the presence of glutamine substrate is consistent with that explanation. Thus, these two modes of inhibition indicate that the transamination reaction is an ordered event dependent on docking of the glutamine substrate to trigger reaction with subsequent binding of the amine substrate.


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Fig. 7.   Model of XIIIA dimer adapted from Yee et al. (17) showing the location of the cross-reacting segment 643-658 situated in barrel 2 through which glutamine substrates are deemed to access the catalytic domain. The illustration was generated using computer programs MolScript (36) and Raster3D (37).

Binding of 5A2 to XIIIa was enhanced by co-incubation with the Berichrome® fXIII substrate system, an indication that substrate binding enhances exposure of the 5A2 epitope on XIIIa. It had been predicted that a conformational change accompanying the glutamine binding functions in the catalytic activity of the enzyme (32). Crystal structures of rXIIIA both before and after activation with either thrombin or high concentrations of calcium (34, 35) led to the inference that the conformational change that must accompany catalysis arises from the docking of the glutamine substrate. Our findings provide evidence for a substrate-induced conformational change and primacy of glutamine binding in the catalytic reaction.

    FOOTNOTES

* This work was supported in part by NHLBI, National Institutes of Health, Grants HL-16361 and HL-50355 and by the Russian Fund for Fundamental Investigation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed: Research Institute FF4, The Cleveland Clinic Foundation, Cleveland, OH 44195. Tel.: 216-444-5851; Fax: 216-445-5480; E-mail: shainoj{at}cesmtp.ccf.org.

1 The abbreviations used are: fXIII, factor XIII; XIIIa, the enzymically active form of blood coagulation fXIII; XIIIA, A-chains of fXIII; rXIIIA, recombinant fXIII A-chains; 5A2, anti-fibrin(ogen) alpha  529-539; mAb, monoclonal antibody; FPA, fibrinopeptide A (fibrinogen Aalpha 1-16); TBSE, Tris-buffered saline with EDTA; TBSTw, Tris-buffered saline with Tween.

2 Fibrinogen alpha -chains (64 kDa) differ from fibrin alpha -chains by containing the N-terminal fibrinopeptide A (1.5 kDa) that blocks the fibrin aggregation site located just penultimate to the fibrinopeptide. The fibrinogen alpha -chains are normally designated as Aalpha -chains except when qualified as fibrinogen rather fibrin chains. We defer the Aalpha convention here to simplify the multimeric structure of the cross-linked chains as alpha gamma gamma trimers rather than Aalpha gamma gamma trimers.

    REFERENCES
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Abstract
Introduction
Materials & Methods
Results
Discussion
References

  1. Lorand, L., Konishi, K., and Jacobsen, A. (1962) Nature 194, 1148-1149
  2. Dadabay, C. Y., and Pike, L. J. (1989) Biochem. J. 264, 679-685[Medline] [Order article via Infotrieve]
  3. Folk, J. E. (1970) Methods Enzymol. 17, 889-894
  4. Folk, J. E. (1980) Annu. Rev. Biochem. 49, 517-531[CrossRef][Medline] [Order article via Infotrieve]
  5. Shainoff, J. R., and Page, I. H. (1962) J. Exp. Med. 116, 687-707
  6. Francis, C. W., and Marder, V. J. (1988) Blood 71, 1361-1365[Abstract]
  7. Sakata, Y., and Aoki, N. (1982) J. Clin. Invest. 69, 536-542[Medline] [Order article via Infotrieve]
  8. Reed, G. L., Matsueda, G. R., and Haber, E. (1992) Thromb. Haemostasis 68, 315-320[Medline] [Order article via Infotrieve]
  9. Barry, E. L., and Mosher, D. F. (1988) J. Biol. Chem. 263, 10464-10469[Abstract/Free Full Text]
  10. Schwartz, M. L., Pizzo, S. V., Hill, R. L., and McKee, P. A. (1973) J. Biol. Chem. 248, 1395-1407[Abstract/Free Full Text]
  11. Lorand, L., and Konishi, K. (1964) Arch. Biochem. Biophys. 105, 58-67
  12. Curtis, C. G., Brown, K. L., Credo, R. B., Domanik, A., Gray, P., Stenberg, P., and Lorand, L. (1974) Biochemistry 13, 3774-3780[Medline] [Order article via Infotrieve]
  13. Laki, K., and Lorand, L. (1948) Science 108, 280
  14. Ichinose, A., and Davie, E. W. (1988) Adv. Exp. Med. Biol. 231, 15-27[Medline] [Order article via Infotrieve]
  15. Ichinose, A., McMullen, B. A., Fujikawa, K., and Davie, E. W. (1986) Biochemistry 25, 4633-4638[Medline] [Order article via Infotrieve]
  16. Ichinose, A., Hendrickson, L. E., Fujikawa, K., and Davie, E. W. (1986) Biochemistry 25, 6900-6906[Medline] [Order article via Infotrieve]
  17. Yee, V. C., Pedersen, L. C., Le Trong, I., Bishop, P. D., Stenkamp, R. E., and Teller, D. C. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 7296-7300[Abstract]
  18. Taubenfeld, S. M., Song, Y., Sheng, D., Ball, E. L., and Matsueda, G. R. (1995) Thromb. Haemostasis 74, 923-927[Medline] [Order article via Infotrieve]
  19. Bishop, P. D., Teller, D. C., Smith, R. A., Lasser, G. W., Gilbert, T., and Seale, R. L. (1990) Biochemistry 28, 1861-1869
  20. Mitkevich, O. V., Sobel, J. H., Shainoff, J. R., Vlasik, T. N., Kalantarov, G. F., Trakht, I. N., Streltsova, Z. A., and Samokhin, G. P. (1996) Blood Coagul. Fibrinolysis 7, 85-92[Medline] [Order article via Infotrieve]
  21. Mitkevich, O. V., Samokhin, G. P., Petrov, A. D., Egorova, E. F., Sergienko, V. B., Malov, A. G., Kuznetsova, E. V., and Zhdanovich, M. Y. (1994) J. Label. Cmpd Radiopharm. 35, 407-409
  22. Valenzuela, R., Shainoff, J. R., DiBello, P. M., Urbanic, D. A., Anderson, J. M., Matsueda, G. R., and Kudryk, B. J. (1992) Am. J. Pathol. 141, 861-880[Abstract]
  23. Danilov, S. M., Muzykantov, V. R., Martynov, A. V., Atochina, E. N., Sakharov, I. Y., Trakht, I. N., and Smirnov, V. N. (1991) Lab. Invest. 64, 118-124[Medline] [Order article via Infotrieve]
  24. Shainoff, J. R., Lahiri, B., and Bumpus, F. M. (1970) Thromb. Diath. Haemorrh. Supplementum 39, 203-217
  25. Shainoff, J. R. (1993) Adv. Electrophor. 6, 61-177
  26. Shainoff, J. R., Urbanic, D. A., and DiBello, P. M. (1991) J. Biol. Chem. 266, 6429-6437[Abstract/Free Full Text]
  27. Lorand, L., Lockridge, O. M., Campbell, L. K., Myhrman, R., and Bruner-Lorand, J. (1971) Anal. Biochem. 44, 2221-2231
  28. Fickenscher, K., Aab, A., and Stuber, W. (1991) Thromb. Haemostasis 65, 535-540[Medline] [Order article via Infotrieve]
  29. Greenberg, C. S., Enghild, J. J., Mary, A., Dobson, J. V., and Achyuthan, K. E. (1988) Biochem. J. 256, 10134-10192
  30. Segel, I. H. (1993) Enzyme Kinetics, p. 329, John Wiley & Sons, Inc., New York
  31. Takahashi, T., Takahashi, Y., and Putnam, F. W. (1986) Proc. Natl. Acad. Sci. U. S. A. 83, 8019-8023[Abstract]
  32. Pedersen, L. C., Yee, V. C., Bishop, P. D., Le Trong, I., Teller, D. C., and Stenkamp, R. E. (1994) Protein Sci. 3, 1131-1135[Abstract/Free Full Text]
  33. Kurochkin, I. V., Procyk, R., Bishop, P. D., Yee, V. C., Teller, D. C., Ingham, K. C., and Medved, L. V. (1995) J. Mol. Biol. 248, 414-430[CrossRef][Medline] [Order article via Infotrieve]
  34. Yee, V. C., Pedersen, L. C., Bishop, P. D., Stenkamp, R. E., and Teller, D. C. (1995) Thromb. Res. 78, 389-397[CrossRef][Medline] [Order article via Infotrieve]
  35. Yee, V. C., Le Trong, I., Bishop, P. D., Pedersen, L. C., Stenkamp, R. E., and Teller, D. C. (1996) Semin. Thromb. Hemostasis 22, 377-384[Medline] [Order article via Infotrieve]
  36. Kraulis, P. J. (1991) J. Appl. Crystallogr. 24, 946-950[CrossRef]
  37. Merritt, E. A., and Murphy, M. E. P. (1998) Acta Crystallogr. Sec. D 50, 869-873


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