ATP-dependent Proteolysis in Mitochondria
m-AAA PROTEASE AND PIM1 PROTEASE EXERT OVERLAPPING SUBSTRATE SPECIFICITIES AND COOPERATE WITH THE mtHsp70 SYSTEM*

Alexander S. Savel'evDagger , Ludmila A. NovikovaDagger , Irina E. KovalevaDagger , Valentin N. LuzikovDagger , Walter Neupert§, and Thomas Langer§

From the § Institut für Physiologische Chemie der Universität München, Goethestr. 33, 80336 München, Germany and the Dagger  A. N. Belozersky Institute of Physico-Chemical Biology, M. V. Lomonosov State University, Moscow 119899, Russian Federation

    ABSTRACT
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Abstract
Introduction
Procedures
Results
Discussion
References

To analyze protein degradation in mitochondria and the role of molecular chaperone proteins in this process, bovine apocytochrome P450scc was employed as a model protein. When imported into isolated yeast mitochondria, P450scc was mislocalized to the matrix and rapidly degraded. This proteolytic breakdown was mediated by the ATP-dependent PIM1 protease, a Lon-like protease in the mitochondrial matrix, in cooperation with the mtHsp70 system. In addition, a derivative of P450scc was studied to which a heterologous transmembrane region was fused at the amino terminus. This protein became anchored to the inner membrane upon import and was degraded by the membrane-embedded, ATP-dependent m-AAA protease. Again, degradation depended on the mtHsp70 system; it was inhibited at non-permissive temperature in mitochondria carrying temperature-sensitive mutant forms of Ssc1p, Mdj1p, or Mge1p. These results demonstrate overlapping substrate specificities of PIM1 and the m-AAA protease, and they assign a central role to the mtHsp70 system during the degradation of misfolded polypeptides by both proteases.

    INTRODUCTION
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Abstract
Introduction
Procedures
Results
Discussion
References

Molecular chaperone proteins bind non-native protein structures and stabilize them against aggregation (1). By this means, they ensure proper folding of newly synthesized proteins, provide protection against heat denaturation, and mediate the vectorial translocation of polypeptides across biological membranes (2-7). Furthermore, evidence is accumulating that chaperone proteins play a pivotal role in ATP-dependent proteolytic processes (8-10). Chaperone and proteolytic activities thereby constitute a quality control system which prevents the possibly deleterious accumulation of misfolded polypeptides in the cell. For instance, the degradation of misfolded polypeptides by Lon-like proteases in Escherichia coli or mitochondria depends on Hsp70 proteins which prevent the aggregation of substrate polypeptides (11-15). In addition to classical chaperone proteins that cooperate with ATP-dependent proteases during proteolysis, intrinsic chaperone-like properties have been assigned to some ATP-dependent proteases themselves which may be crucial for the degradation of non-native polypeptides (9, 10). The best studied cases are the hetero-oligomeric Clp proteases of prokaryotes whose regulatory subunits exert ATP-dependent chaperone activity (16, 17).

Several ATP-dependent proteases have been identified in mitochondria, which mediate the selective degradation of proteins in this organelle (10, 18, 19). These proteases are required for maintenance of the respiratory competence of yeast cells suggesting important regulatory functions during the biogenesis of mitochondria. An ATP-dependent protease, highly homologous to Escherichia coli Lon protease, has been identified in the mitochondrial matrix space (20, 21). The corresponding genes from humans (22, 23) and yeast (24, 25) were cloned and termed PIM1 (for proteolysis in mitochondria) or LON. PIM1-mediated proteolysis is required for the maintenance of mitochondrial genome integrity (24, 25) and for the synthesis of mitochondrially encoded subunits of the respiratory chain (70). PIM1 protease forms a high molecular weight, presumably homo-oligomeric complex whose integrity depends on ATP and that mediates the degradation of misfolded polypeptides in the matrix space (26). Proteolysis requires the function of the mitochondrial Hsp70 protein Ssc1p and its co-chaperone Mdj1p, which stabilize substrate polypeptides in a conformation susceptible to degradation by PIM1 protease (15).

Proteins of the inner membrane of mitochondria that are not assembled to functional complexes are degraded by two AAA proteases (27-32). The subunits of these membrane-bound and metal-dependent proteases harbor characteristic, conserved ATPase domains (33, 34). The two known mitochondrial AAA proteases form high molecular weight complexes and expose their catalytic sites to opposite surfaces of the inner membrane: Yme1p, the solely identified subunit of the i-AAA protease, is active on the outer surface (35). In contrast, the catalytic sites of the m-AAA protease, composed of several copies of Yta10p (Afg3p) and Yta12p (Rca1p), are facing the mitochondrial matrix (28, 36). The m-AAA protease was found to stably interact with non-native substrate polypeptides suggesting a chaperone-like activity of this protease (28).

In the present manuscript, we report on the requirement of the mitochondrial Hsp70 system for the degradation of misfolded polypeptides by the PIM1 and the m-AAA protease. We exploited the observation that bovine apocytochrome P450scc, missorted to the matrix after import into yeast mitochondria, and a membrane-associated derivative thereof were subject to rapid proteolysis. While P450scc was degraded by PIM1 protease in the matrix, the proteolytic breakdown of the membrane-anchored variant was mediated by the m-AAA protease demonstrating overlapping substrate specificities of both proteases. In either case, proteolysis depended on the activity of the molecular chaperones Ssc1p and Mdj1p and on Mge1p, which acts as a nucleotide exchange factor for Ssc1p. Thus, the mtHsp70 system can cooperate with different ATP-dependent proteases in the degradation of non-native polypeptides in mitochondria.

    EXPERIMENTAL PROCEDURES
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Abstract
Introduction
Procedures
Results
Discussion
References

Construction of pSu9(112)-P450scc-- Recombinant DNA techniques were applied as described previously (37, 38). To generate pSu9(112)-P450scc, a DNA fragment encoding mouse dihydrofolate reductase was excised from Su9(112)-dihydrofolate reductase cloned in pGEM4 (39) by restriction digest with BamHI and HindIII. The HindIII site was filled in with Klenow and the vector was ligated with a BamHI-SmaI fragment of the plasmid pYeDP/coxIV-P450scc (40) encoding mature bovine P450scc lacking the N-terminal 75 residues. The resulting hybrid protein consisted of the 112 N-terminal amino acids of the F0-ATPase subunit 9 fused to truncated P450scc.

Yeast Strains and Growth Conditions-- Yeast strains carrying mutations in PIM1 were previously described (26). To establish the dependence on the proteolytic activity, PIM1 and a proteolytically inactive variant, PIM1S1015A, were expressed in Delta pim1 cells from a multicopy plasmid under the control of a galactose-inducible promotor (26). Cells were grown at 30 °C on selective medium containing 2% glucose and 0.5% galactose to induce expression from the GAL1 promotor. Wild type and mutant PIM1 protease were approximately 30-fold overexpressed in these cells (26). Yeast strains carrying mutations in YTA10 or YTA12 are derivatives of W303-1A and are described elsewhere (28). yta10 or yta12 mutant strains were grown on YP medium supplemented with 2% galactose and 0.5% lactate at 30 °C according to standard procedures. Yeast strains carrying the temperature-sensitive mutant allele ssc1-3 (41) or mge1-3 (42) were grown at 24 °C on selective medium containing 2% galactose and 0.5% lactate. The mdj1-7 mutant strain (43) was grown at 24 °C on lactate medium containing galactose instead of glucose.

Import of Preproteins into Isolated Mitochondria and Protein Degradation-- Mitochondria were isolated as described previously except that zymolyase treatment was performed at 24 °C in the case of temperature-sensitive strains (44, 45). pP450scc and pSu9(112)-P450scc were transcribed using SP6 polymerase and synthesized in rabbit reticulocyte lysate in the presence of [35S]methionine (Promega) according to standard procedures (46). Import of the radiolabeled preproteins was performed for 25 min at 25 °C essentially as described (15) and halted by the addition of valinomycin (2 µM). To allow for proteolysis of newly imported proteins, mitochondria were further incubated in import buffer at 37 °C. At the time points indicated, aliquots were withdrawn and diluted 3-fold with ice-cold SHKCl (50 mM HEPES/KOH, pH 7.4, 0.6 M sorbitol, 80 mM KCl). To digest non-imported precursor proteins, samples were treated with trypsin (75 µg/ml) for 20 min at 4 °C when indicated. Protease digestion was inhibited by adding a 20-fold excess (w/w) of soybean trypsin inhibitor. Mitochondria were then reisolated by centrifugation, washed with SHKCl containing 1 mM phenylmethylsulfonyl fluoride and analyzed by SDS-PAGE.1

Subfractionation of Mitochondria-- The mitochondrial outer membrane was disrupted by osmotic swelling, and mitochondrial proteins were extracted by sodium carbonate pH 11.5 as described previously (36). Subfractionation of mitochondria by digitonin treatment was performed in the presence of proteinase K (50 µg/ml) according to published procedures (47).

    RESULTS
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Abstract
Introduction
Procedures
Results
Discussion
References

Bovine Apocytochrome P450scc Is Imported into Yeast Mitochondria, but Is Not Correctly Sorted to the Inner Membrane-- The precursor of bovine apocytochrome P450scc (pP450scc) was synthesized in rabbit reticulocyte lysate in the presence of [35S]methionine and imported into isolated yeast mitochondria in a membrane potential-dependent manner (data not shown). Upon import, the precursor form was converted with low efficiency into a mature form most likely mediated by the mitochondrial processing peptidase (48). Cytochrome P450scc molecules are integral parts of the inner membrane in adrenocortical mitochondria (49). To test the membrane association of newly imported bovine apocytochrome P450scc, yeast mitochondria were treated with carbonate at pH 11.5. This treatment results in the release of soluble proteins into the supernatant fraction, while integral membrane proteins, such as the ADP/ATP carrier, are resistant toward extraction under these conditions. P450scc was almost exclusively recovered from the soluble fraction upon alkaline extraction of the mitochondria, similar to cytochrome b2 and Mge1p, soluble proteins of the intermembrane and matrix space, respectively (Fig. 1A). Apparently, bovine P450scc is not inserted into the inner membrane of yeast mitochondria. To localize P450scc, yeast mitochondria were treated with increasing amounts of digitonin in the presence of externally added protease. The intermembrane and matrix space became successively accessible to the protease as indicated by the marker proteins cytochrome b2 (for the intermembrane space) and Mge1p (for the matrix space) (Fig. 1B). Newly imported precursor and mature P450scc were released from mitochondria in parallel with Mge1p indicating that both forms are localized in the mitochondrial matrix (Fig. 1B). These results demonstrate that bovine apocytochrome P450scc is imported into yeast mitochondria and, although with low efficiency, processed to the mature form. In contrast to adrenocortical mitochondria, however, P450scc molecules are not inserted into the inner membrane but accumulate in the matrix space. Thus, intramitochondrial sorting appears to depend on a specific sorting pathway in adrenocortical mitochondria or on the lipid composition of the membrane. It should be noted, however, that at least a fraction of P450scc appears to be correctly inserted into the inner mitochondrial membrane after expression in Saccharomyces cerevisiae, as submitochondrial particles exhibited cholesterol hydroxylase activity in the presence of externally added adrenodoxin and adrenodoxin reductase (40).


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Fig. 1.   Localization of bovine pP450scc in yeast mitochondria. A, subfractionation of mitochondria by alkaline extraction. Newly synthesized, [35S]methionine-labeled pP450scc was imported into isolated yeast mitochondria for 25 min at 25 °C, and non-imported preproteins were digested with trypsin as described under "Experimental Procedures." Mitochondria were then resuspended in 0.1 M Na2CO3, pH 11.5, and 1 mM phenylmethylsulfonyl fluoride and separated into soluble and pellet fractions by centrifugation as described previously (36). Protein fractions were analyzed by SDS-PAGE, followed by autoradiography and immunoblotting using polyclonal antisera directed against cytochrome b2 (cyt b2), the ADP/ATP carrier (AAC), and Mge1p. T, total; P, pellet fraction; S, supernatant fraction. B, digitonin fractionation of mitochondria. After import of pP450scc and trypsin digestion, mitochondria were treated with increasing amounts of digitonin in the presence of proteinase K. After centrifugation, proteins in the pellet fraction were analyzed by SDS-PAGE and blotted onto nitrocellulose. Immunoblotting of the endogenous marker proteins cytochrome b2 (Cyt b2) (open circle ) and Mge1p (bullet ) was performed using a chemiluminescence detection kit and quantified by laser densitometry. The amount of the protein in samples incubated with proteinase K in the absence of digitonin was set to 100%. black-triangle, pP450scc, precursor form of P450scc; triangle , mP450scc, mature form of P450scc.

ATP-dependent Degradation of P450scc by PIM1 Protease in the Matrix-- The stability of newly imported, missorted apocytochrome P450scc was analyzed in further experiments. After import of P450scc, the electrochemical gradient across the mitochondrial inner membrane was dissipated by adding the uncoupler valinomycin, and then mitochondria were incubated at 37 °C. Newly imported P450scc was degraded in mitochondria in a time-dependent fashion (Fig. 2). Proteolysis was impaired upon depletion of mitochondrial matrix ATP (Fig. 2).


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Fig. 2.   ATP-dependent degradation of newly imported P450scc. Newly synthesized pP450scc was imported for 25 min at 25 °C into isolated mitochondria as described under "Experimental Procedures." Import was halted by the addition of valinomycin (2 µM), and samples were further incubated at 37 °C in import buffer in the presence of an ATP-regenerating system (10 mM phosphocreatine, 100 µg/ml creatine kinase)(+ATP) or in the presence of oligomycin (20 µM) and apyrase (40 units/ml)(-ATP). At the time points indicated, aliquots were withdrawn and non-imported precursor proteins were digested with trypsin. Samples were analyzed by SDS-PAGE and autoradiography. p, precursor form of P450scc; m, mature form of P450scc.

To identify the protease responsible for the proteolytic breakdown of P450scc, mitochondria were isolated from yeast strains carrying mutations in either the PIM1 or the m-AAA protease, and the stability of newly imported P450scc was examined. Inactivation of either protease does not affect the accumulation of the other protease in mitochondria excluding indirect effects of the mutations (data not shown). Proteolysis of P450scc was impaired in mitochondria lacking PIM1 protease (Fig. 3A). Impairment of proteolysis of P450scc might result from a disturbed energy metabolism in pim1 mutant cells, which accumulate lesions in mtDNA and therefore are respiratory-deficient (24, 25). To exclude this possibility, the stability of P450scc was examined in Delta pim1 mitochondria which lack mtDNA but contain PIM1 protease. Wild type and the proteolytically inactive variant PIM1S1015A protease carrying a mutation in the proteolytic center (26) were expressed in Delta pim1 cells. Newly imported P450scc was degraded, although with reduced efficiency, in Delta pim1 mitochondria harboring the wild type form of the protease, while proteolysis was not restored upon expression of PIM1S1015A protease (Fig. 3A). Thus, degradation of P450scc depends on the proteolytic activity of PIM1. In contrast, degradation of P450scc was not significantly affected by mutations in the catalytic sites of Yta10p (E559Q) or Yta12p (E614Q), which are subunits of the membrane-bound m-AAA protease (Fig. 3B). Both mutations have previously been observed to abolish the proteolytic breakdown of non-assembled polypeptides in the inner membrane by the m-AAA protease (27, 28). We conclude from these experiments that missorted P450scc is degraded by PIM1 protease in the mitochondrial matrix space.


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Fig. 3.   Degradation of P450scc depends on the presence of a proteolytically active PIM1 protease in mitochondria but is not affected by mutations in m-AAA protease subunits. pP450scc was imported into mitochondria isolated from a Delta pim1 strain () and from Delta pim1 strains expressing PIM1 (Delta pim1/PIM1)(open circle ) or PIM1S1015A (pim1S1015A)(black-square) (A) or from wild type cells (WT)(open circle ), from a Delta yta10 strain expressing YTA10E559Q (yta10E559Q)(), and from a Delta yta12 strain expressing YTA12E614Q (yta12E614Q)(black-square) (B). After inhibition of import by adding valinomycin, samples were further incubated at 37 °C in the presence of an ATP-regenerating system and analyzed as described in Fig. 2. The total amount of imported mature and precursor forms of P450scc prior to incubation at 37 °C was set to 100%.

Missorted P450scc Is Prone to Aggregation in Mitochondria-- Newly imported P450scc, missorted to the mitochondrial matrix, most likely cannot attain its native conformation. It is therefore conceivable that P450scc is prone to aggregation in yeast mitochondria. P450scc, however, was recovered in the soluble fraction of mitochondria, even when these were incubated at 37 °C for 15 min (Fig. 4). Likewise, the solubility of P450scc was not affected after import into mitochondria lacking Yta10p or Yta12p and therefore m-AAA protease activity (Fig. 4). In contrast, P450scc formed insoluble aggregates in mitochondria lacking PIM1 protease (Fig. 4). These results demonstrate that missorted P450scc is prone to aggregation in yeast mitochondria and further substantiate the requirement of PIM1 protease for the proteolytic breakdown of P450scc.


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Fig. 4.   Aggregation of newly imported P450scc in the absence of a proteolytically active PIM1 protease. After import of P450scc and addition of valinomycin, mitochondria were incubated for 15 min at 37 °C, treated with trypsin to digest non-imported preproteins, and lysed for 15 min at 4 °C in 0.1% (w/v) Triton X-100, 10 mM MOPS/KOH, pH 7.2, 150 mM NaCl, 5 mM EDTA, 0.5 mM phenylmethylsulfonyl fluoride at a concentration of 1 mg/ml. Aggregation of P450scc was assessed by centrifugation for 15 min at 25,000 × g. The pellet and supernatant fractions were analyzed by SDS-PAGE and autoradiography. P450scc in the pellet fraction is given as percent of imported P450scc prior to incubation at 37 °C. Similar aggregation of the precursor and mature form of P450scc was observed in all strains.

The aggregation of P450scc in the absence of PIM1 might reflect a deficiency in a chaperone-like function of the protease, which has recently been proposed to exist independent of its proteolytic activity (50). Therefore, proteolytically inactive PIM1S1015A was overexpressed in pim1 null mutant cells, and aggregation of newly imported P450scc was analyzed (Fig. 4). Replacement of the conserved serine 1015 by alanine abolishes proteolytic activity of PIM1 but does not affect the overall protein stability nor the ATP-dependent assembly of the protease subunits (26, 50). PIM1S1015A protease did not stabilize P450scc against aggregation (Fig. 4), although it was present at an approximately 30-fold higher level in mitochondria when compared with wild type cells (26). Apparently, PIM1 protease on its own is not capable of maintaining missorted P450scc in a soluble state.

Requirement of the mtHsp70 System for the PIM1-mediated Degradation of P450scc-- To examine a possible role of the mitochondrial Hsp70 protein Ssc1p in preventing aggregation of P450scc, we used mitochondria from the ssc1-3 yeast strain containing a temperature-sensitive mutant form of Ssc1p (41). Furthermore, the stability of P450scc was analyzed in mitochondria carrying a conditional mutant form of Mdj1p (mdj1-7) (43), which also seems to act as a molecular chaperone in mitochondria and ensures efficient binding to and release from Ssc1p (15, 51). After import of pP450scc into mitochondria at permissive temperature, samples were incubated at 37 °C to inactivate the mutant forms of the proteins and to allow degradation to occur. While P450scc was degraded in wild type mitochondria, proteolysis was impaired in ssc1-3 and mdj1-7 mutant mitochondria at non-permissive temperatures (Fig. 5A). P450scc formed insoluble aggregates under these conditions (Fig. 5B). Thus, similar to other misfolded proteins, the degradation of P450scc depends on Ssc1p and Mdj1p which stabilize P450scc molecules against aggregation and thereby allow their proteolytic breakdown by PIM1.


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Fig. 5.   PIM1-mediated proteolysis of newly imported P450scc depends on the mtHsp70 system. pP450scc was imported into wild type (WT) (bullet ), ssc1-3 (triangle ), mdj1-7 (open circle ), and mge1-3 (black-triangle) mutant mitochondria for 25 min at 25 °C as described under "Experimental Procedures." A, degradation of P450scc is impaired in ssc1-3, mdj1-7, and mge1-3 mutant mitochondria at non-permissive temperature. After import at permissive temperature, samples were further incubated at 37 °C, and the stability of P450scc was analyzed as described in Fig. 2. B, newly imported P450scc aggregates in ssc1-3 and mdj1-7, but not in mge1-3 mutant mitochondria at non-permissive temperature. After completion of import, mitochondria were incubated for 15 min at 37 °C. Aggregation of P450scc was analyzed as described in Fig. 3C.

Polypeptide binding to Ssc1p is also regulated by Mge1p, which acts as a nucleotide exchange factor for Ssc1p (42, 52-54). A possible role of Mge1p during proteolysis of misfolded polypeptides in mitochondria, however, has not been reported until now. The degradation of P450scc was therefore analyzed in mitochondria carrying the temperature-sensitive mutant allele mge1-3 (42). Inactivation of Mge1-3p at non-permissive temperature resulted in the stabilization of newly imported P450scc (Fig. 5A). In contrast to ssc1-3 or mdj1-7 mitochondria, P450scc accumulated in a soluble form in mge1-3 mitochondria (Fig. 5B), most likely due to an impaired release of P450scc from Ssc1p (52). These results establish a crucial function of all components of the mtHsp70 system during the PIM1-mediated proteolysis of misfolded proteins in mitochondria.

An Apocytochrome P450scc Variant with an Amino-terminal Transmembrane Region Is Anchored to the Inner Membrane-- To ensure sorting of bovine apocytochrome P450scc to the mitochondrial inner membrane, the targeting sequence and the amino-terminal 75 amino acid residues of P450scc were replaced by the first 112 amino acids of the subunit 9 of the F1F0-ATPase of Neurospora crassa. This sequence contains the mitochondrial targeting sequence and the first transmembrane region of ATPase subunit 9 and has previously been used to target heterologous proteins to the inner membrane (55). Import of the resulting hybrid protein, Su9(112)-P450scc, depended on an electrochemical potential across the mitochondrial inner membrane (Fig. 6A). To determine the submitochondrial localization of Su9(112)-P450scc, mitochondria were fractionated by osmotic swelling (Fig. 6B). Su9(112)-P450scc was not released into the supernatant upon disruption of the outer membrane nor degraded by protease which was externally added to mitoplasts suggesting its presence in an internal mitochondrial compartment (Fig. 6B). The association of Su9(112)-P450scc with the inner membrane was examined by treatment of mitochondria with carbonate at pH 11.5 (Fig. 6B). In contrast to P450scc, the majority of newly imported Su9(112)-P450scc was recovered from the pellet fraction after extraction of mitochondria indicating the insertion of the protein into the inner membrane. In agreement with these findings, Su9(112)-P450scc became solubilized in parallel with the ADP/ATP carrier when mitochondria were treated with increasing amounts of digitonin (data not shown). We conclude from these experiments that Su9(112)-P450scc is tightly associated with the inner membrane.


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Fig. 6.   Import and localization of Su9(112)-P450scc in yeast mitochondria. A, Su9(112)-P450scc is imported into mitochondria in a Delta psi -dependent manner. Newly synthesized Su9(112)-P450scc was imported into isolated mitochondria for 20 min at 25 °C in the presence (-Delta psi ) or absence (+Delta psi ) of valinomycin (0.5 µM), oligomycin (20 µM), and antimycin A (8 µM). Non-imported preproteins were digested with trypsin when indicated. p, precursor form of Su9(112)-P450scc; m, mature form of Su9(112)-P450scc. B, newly imported Su9(112)-P450scc is sorted to the mitochondrial inner membrane. After import of Su9(112)-P450scc for 20 min at 25 °C, the outer membrane was disrupted by osmotic swelling in the presence or absence of proteinase K (20 µg/ml)(PK) as indicated. In parallel, mitochondria were extracted with 0.1 M Na2CO3, pH 11.5, and split into soluble and pellet fractions by centrifugation as in Fig. 1. Proteins in both fractions were separated by SDS-PAGE, blotted onto nitrocellulose, and analyzed by autoradiography and immunoblotting using cytochrome b2 (cyt b2), the ADP/ATP-carrier (AAC), and Mge1p as marker proteins.

Membrane-anchored P450scc Is Degraded by the m-AAA Protease-- Sonication of adrenocortical mitochondria in the presence of trypsin results in the clipping of endogenous cytochrome P450scc in two fragments of 26 and 29 kDa, which is considered to indicate a native conformation of the protein in the inner membrane (56, 57). Newly imported Su9(112)-P450scc, however, was completely degraded by trypsin when mitochondria were lysed by sonication (data not shown). Although attached to the inner membrane, the P450scc moiety of the hybrid protein apparently does not fold into its native conformation and is most likely exposed to the matrix. Therefore, the stability of Su9(112)-P450scc in mitochondria was examined in further experiments. The membrane-anchored fusion protein was degraded in wild type mitochondria (Fig. 7A). Su9(112)-P450scc was, however, stabilized in mitochondria lacking proteolytically active m-AAA protease: proteolysis was impaired in Delta yta10 and Delta yta12 mitochondria expressing proteolytically inactive forms of either subunit of the m-AAA protease, Yta10E559Qp or Yta12E614Qp (Fig. 7A). In contrast, the proteolytic breakdown of Su9(112)-P450scc occurred with similar kinetics in Delta pim1 mitochondria expressing either the wild type or the proteolytically inactive form of the PIM1 protease (Fig. 7B). Thus, membrane-anchored Su9(112)-P450scc, in contrast to matrix-located P450scc, is degraded by the m-AAA protease.


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Fig. 7.   Degradation of Su9(112)-P450scc in the mitochondrial inner membrane is mediated by the m-AAA protease. A, the stability of Su9(112)-P450scc in mitochondria containing proteolytically inactive Yta10p and Yta12p. Su9(112)-P450scc was imported for 20 min at 25 °C into mitochondria isolated from wild type cells (WT)(bullet ), from a Delta yta10 strain expressing YTA10E559Q (yta10E559Q)(), and from a Delta yta12 strain expressing YTA12E614Q (yta12E614Q)(n) as described under "Experimental Procedures." Proteolysis of newly imported Su9(112)-P450scc at 37 °C was determined as in Fig. 3. B, degradation of Su9(112)-P450scc in the presence of proteolytically inactive PIM1 protease. Su9(112)-P450scc was imported into Delta pim1 mutant mitochondria harboring wild type PIM1 protease (Delta pim1/PIM1)(open circle ) or the proteolytically inactive PIM1S1015A protease (pim1S1015A)(black-square), and the stability at 37 °C was determined as described in Fig. 3.

Degradation of Membrane-anchored P450scc by the m-AAA Protease Depends on the mtHsp70 System-- It is conceivable that molecular chaperones are required to maintain the matrix-exposed domain of Su9(112)-P450scc in a conformation susceptible to degradation by the m-AAA protease. To analyze the involvement of the mtHsp70 system in the proteolysis of membrane-anchored P450scc, Su9(112)-P450scc was imported at permissive temperature into ssc1-3, mdj1-7, and mge1-3 mutant mitochondria. These conditions allow import of Su9(112)-P450scc into mitochondria and its insertion into the inner membrane. The stability of newly imported, membrane-associated Su9(112)-P450scc was then examined at non-permissive temperature (Fig. 8). While Su9(112)-P450scc was degraded in wild type mitochondria, proteolysis was impaired upon inactivation of Ssc1p, Mdj1p, or Mge1p in ssc1-3, mdj1-7, or mge1-3 mitochondria, respectively. These results demonstrate that degradation of membrane-bound Su9(112)-P450scc by the m-AAA protease depends on Ssc1p and the co-chaperones Mdj1p and Mge1p which act after insertion of the polypeptide into the inner membrane.


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Fig. 8.   The proteolytic breakdown of Su9(112)-P450scc by the m-AAA protease depends on the mtHsp70 system. Su9(112)-P450scc was imported into wild type (WT)(open circle ), ssc1-3 (triangle ), mdj1-7 (open circle ), and mge1-3 (triangle ) mutant mitochondria for 25 min at 25 °C as described under "Experimental Procedures." After inhibition of import, samples were incubated at 37 °C and proteolysis was determined as in Fig. 2.

    DISCUSSION
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Abstract
Introduction
Procedures
Results
Discussion
References

Molecular chaperone proteins fulfill essential functions during the proteolysis of misfolded polypeptides in mitochondria. The mtHsp70 system cooperates not only with PIM1 protease in the degradation of non-native polypeptides accumulating in the matrix, but in addition is required for the proteolytic breakdown of at least some polypeptides which are associated with the inner membrane and degraded by the m-AAA protease. The proteolysis of misfolded polypeptides depends on the activities of Ssc1p and Mdj1p, which both exert chaperone activity and thereby most likely prevent the irreversible misfolding of polypeptides prone to degradation. Moreover, we assign a crucial role for the proteolysis of misfolded proteins to Mge1p, which acts as a nucleotide exchange factor of Ssc1p.

The mtHsp70 system is required for both folding and degradation of mitochondrial proteins and thereby comprises a quality control system in mitochondria. The experiments described in the present manuscript, together with previous findings (8, 9, 15, 19), suggest that folding-competent polypeptides are distinguished from irreversibly misfolded polypeptides by kinetic partitioning. Newly imported preproteins attain their native state in the matrix upon cycles of binding to and release from Ssc1p. Folding occurs upon release from the chaperone in the unbound state or in association with the mitochondrial Hsp60 machinery (6, 58, 59). The ATP-dependent binding of a polypeptide to Ssc1p is regulated by Mdj1p and Mge1p. Irreversibly misfolded polypeptides undergo additional binding and release cycles and remain associated with the mtHsp70 system for a prolonged time. This may allow the recognition of the polypeptides by the protease and, after release from the chaperone protein, their degradation. A role of the mtHsp70 system for the folding of inner membrane proteins remains to be examined; however, our results suggest that soluble and at least some membrane-embedded proteins are affected similarly by chaperone proteins. The functional cooperation of the mtHsp70 system with two different ATP-dependent proteases suggests that no specific recognition of chaperone proteins by the proteases occurs and is therefore in agreement with a kinetic partitioning of substrate polypeptides between chaperone proteins and proteases.

The function of Mge1p is essential for the degradation of misfolded proteins by different proteases in mitochondria, most likely as it modulates their binding to Ssc1p (42, 51, 52, 60). While polypeptides prone to degradation form aggregates if the activity of the chaperone proteins Ssc1p and Mdj1p is impaired, they accumulate in the soluble state in the absence of Mge1p function. Substrate polypeptides apparently are not released from the chaperone proteins under these conditions. Mge1p promotes the release of ADP from Ssc1p allowing the binding of ATP to the chaperone (52-54). Upon ATP binding, substrate polypeptides dissociate from Hsp70 proteins (61-63). Thus, impaired nucleotide release from Ssc1p in the absence of Mge1p inhibits the release of associated substrate polypeptides, a prerequisite for their degradation.

The m-AAA protease stably binds non-native, integral membrane proteins which are prone to degradation suggesting chaperone-like properties of the protease (28). A chaperone-like activity of the protease might be needed to stabilize polypeptides in a conformation susceptible to proteolysis. This might include unfolding of hydrophilic domains or the extraction of transmembrane segments from the lipid bilayer. Nevertheless, degradation of at least some membrane-associated polypeptides by the m-AAA protease depends, in addition, on the mtHsp70 system. This finding is reminiscent of studies in E. coli which demonstrate the requirement of the DnaK system for the degradation of the soluble transcription factor sigma 32 by FtsH, a homologue of the mitochondrial m-AAA protease (64-67). It is conceivable that molecular chaperones are specifically required to prevent misfolding of large, solvent-exposed domains of membrane-associated polypeptides, while proteolysis of other membrane proteins by the m-AAA protease is independent of the mtHsp70 system. Indeed, the proteolytic breakdown by the m-AAA protease of non-assembled, mitochondrially encoded subunits of respiratory chain complexes, which lack large solvent-exposed domains, was not affected in ssc1-3 mutant mitochondria at non-permissive temperature.2

Our results demonstrate an overlapping substrate specificity of the m-AAA protease with the PIM1 protease. A misfolded polypeptide is degraded by either protease depending on its localization in mitochondria. This finding is in agreement with genetic evidence indicating functional similarities between both proteases (50, 68). Next to nothing is known about the sequence specificity of ATP-dependent proteases in mitochondria. Studies on the E. coli Lon protease, however, suggest a rather degenerate specificity of Lon-like proteases (69). Similarly, the analysis of proteolytic fragments of a model protein generated by the i- and m-AAA protease did not reveal a conserved cleavage motif (35). The degradation of mitochondrial proteins by ATP-dependent proteases is apparently mainly initiated by a non-native conformation of the polypeptide. Molecular chaperones and chaperone-like properties of the proteases may therefore play a crucial role in the recognition of substrate polypeptides.

    ACKNOWLEDGEMENTS

We are grateful to Dr. M. Waterman (University of Texas) for the bovine cytochrome P450scc cDNA clone, to Dr. S. A. Usanov (Minsk) for purified bovine cytochrome P450scc, and to Dr. E. Craig for the ssc1-3 mutant strain. The excellent technical assistance of Petra Robisch and Alexandra Weinzierl is gratefully acknowledged.

    FOOTNOTES

* This work was supported by Grant 96-48376 from the Russian Foundation for Basic Research and Grant 1/70516 from the Volkswagenstiftung (to V. N. L.) and by Grants La918/1-2 and SFB 184/B21 from the Deutsche Forschungsgemeinschaft (to T. L.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 49-89-5996-283; Fax: 49-89-5996-270; E-mail: Langer{at}bio.med.uni-muenchen.de.

The abbreviations used are: PAGE, polyacrylamide gel electrophoresis; MOPS, 4-morpholinepropanesulfonic acid .

2 T. Langer, unpublished observations.

    REFERENCES
Top
Abstract
Introduction
Procedures
Results
Discussion
References

  1. Ellis, R. J. (1987) Nature 328, 378-379[CrossRef][Medline] [Order article via Infotrieve]
  2. Hartl, F. U. (1996) Nature 381, 571-579[CrossRef][Medline] [Order article via Infotrieve]
  3. Fenton, W. A., and Horwich, A. L. (1997) Protein Sci. 6, 743-760[Abstract/Free Full Text]
  4. Johnson, J. L., and Craig, E. A. (1997) Cell 90, 201-204[Medline] [Order article via Infotrieve]
  5. Parsell, D. A., and Lindquist, S. (1994) in The Biology of Heat Shock Proteins and Molecular Chaperones (Morimoto, R. I., Tissières, A., and Georgopoulos, C., eds), pp. 457-494, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
  6. Rassow, J., Voos, W., and Pfanner, N. (1995) Trends Cell Biol. 5, 207-212[CrossRef]
  7. Brodsky, J. L. (1996) Trends Biochem. Sci. 21, 122-126[CrossRef][Medline] [Order article via Infotrieve]
  8. Hayes, S. A., and Dice, J. F. (1996) J. Cell Biol. 132, 255-258[Medline] [Order article via Infotrieve]
  9. Gottesman, S., Wickner, S., and Maurizi, M. (1997) Genes Dev. 11, 815-823[CrossRef][Medline] [Order article via Infotrieve]
  10. Suzuki, C. K., Rep, M., Van Dijl, J. M., Suda, K., Grivell, L. A., and Schatz, G. (1997) Trends Biochem. Sci. 22, 118-123[CrossRef][Medline] [Order article via Infotrieve]
  11. Straus, D. B., Walter, W. A., and Gross, C. A. (1988) Genes Dev. 2, 1851-1858[Abstract]
  12. Keller, J. A., and Simon, L. D. (1988) Mol. Microbiol. 2, 31-41[Medline] [Order article via Infotrieve]
  13. Sherman, M., and Goldberg, A. L. (1992) EMBO J. 11, 71-77[Abstract]
  14. Jubete, Y., Maurizi, M. R., and Gottesman, S. (1996) J. Biol. Chem. 271, 30798-30803[Abstract/Free Full Text]
  15. Wagner, I., Arlt, H., van Dyck, L., Langer, T., and Neupert, W. (1994) EMBO J. 13, 5135-5145[Abstract]
  16. Schirmer, E. C., Glover, J. R., Singer, M. A., and Lindquist, S. (1996) Trends Biochem. Sci. 21, 289-296[CrossRef][Medline] [Order article via Infotrieve]
  17. Wawrzynow, A., Banecki, B., and Zylicz, M. (1996) Mol. Microbiol. 21, 895-899[Medline] [Order article via Infotrieve]
  18. Rep, M., and Grivell, L. A. (1996) Curr. Genet. 30, 367-380[CrossRef][Medline] [Order article via Infotrieve]
  19. Langer, T., and Neupert, W. (1996) Experientia 52, 1069-1076[Medline] [Order article via Infotrieve]
  20. Desautels, M., and Goldberg, A. L. (1982) J. Biol. Chem. 257, 11673-11679[Free Full Text]
  21. Kutejová, E., Durcová, G., Surovková, E., and Kuzela, S. (1993) FEBS Lett. 329, 47-50[CrossRef][Medline] [Order article via Infotrieve]
  22. Wang, N., Gottesman, S., Willingham, M. C., Gottesman, M. M., and Maurizi, M. R. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 11247-11251[Abstract]
  23. Wang, N., Maurizi, M. R., Emmert-Buck, L., and Gottesman, M. M. (1994) J. Biol. Chem. 269, 29308-29313[Abstract/Free Full Text]
  24. Van Dyck, L., Pearce, D. A., and Sherman, F. (1994) J. Biol. Chem. 269, 238-242[Abstract/Free Full Text]
  25. Suzuki, C. K., Suda, K., Wang, N., and Schatz, G. (1994) Science 264, 273-276[Medline] [Order article via Infotrieve]
  26. Wagner, I., Van Dyck, L., Savel'ev, A., Neupert, W., and Langer, T. (1997) EMBO J. 16, 7317-7325[Abstract/Free Full Text]
  27. Guélin, E., Rep, M., and Grivell, L. A. (1996) FEBS Lett. 381, 42-46[CrossRef][Medline] [Order article via Infotrieve]
  28. Arlt, H., Tauer, R., Feldmann, H., Neupert, W., and Langer, T. (1996) Cell 85, 875-885[Medline] [Order article via Infotrieve]
  29. Tzagoloff, A., Yue, J., Jang, J., and Paul, M. F. (1994) J. Biol. Chem. 269, 26144-26151[Abstract/Free Full Text]
  30. Nakai, T., Yasuhara, T., Fujiki, Y., and Ohashi, A. (1995) Mol. Cell. Biol. 15, 4441-4452[Abstract]
  31. Pearce, D. A., and Sherman, F. (1995) J. Biol. Chem. 270, 20879-20882[Abstract/Free Full Text]
  32. Weber, E. R., Hanekamp, T., and Thorsness, P. E. (1996) Mol. Biol. Cell 7, 307-317[Abstract]
  33. Kunau, W. H., Beyer, A., Franken, T., Gotte, K., Marzioch, M., Saidowsky, J., Skaletz-Rorowski, A., and Wiebel, F. F. (1993) Biochimie (Paris) 75, 209-224[CrossRef][Medline] [Order article via Infotrieve]
  34. Beyer, A. (1997) Protein Sci. 6, 2043-2058[Abstract/Free Full Text]
  35. Leonhard, K., Herrmann, J. M., Stuart, R. A., Mannhaupt, G., Neupert, W., and Langer, T. (1996) EMBO J. 15, 4218-4229[Abstract]
  36. Pajic, A., Tauer, R., Feldmann, H., Neupert, W., and Langer, T. (1994) FEBS Lett. 353, 201-206[CrossRef][Medline] [Order article via Infotrieve]
  37. Ausubel, F. J., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. (1992) Current Protocols in Molecular Biology, Greene Publishing Associates and Wiley-Interscience, New York
  38. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
  39. Ungermann, C., Neupert, W., and Cyr, D. M. (1994) Science 266, 1250-1253[Medline] [Order article via Infotrieve]
  40. Savel'ev, A. S., Novikova, L. A., Drutsa, V. L., Isaeva, L. V., Chernogolov, A. A., Usanov, S. A., and Luzikov, V. N. (1997) Biochemistry (Mosc.) 62, 779-786[Medline] [Order article via Infotrieve]
  41. Gambill, B. D., Voos, W., Kang, P. J., Miao, B., Langer, T., Craig, E. A., and Pfanner, N. (1993) J. Cell Biol. 123, 109-117[Abstract]
  42. Westermann, B., Prip-Buus, C., Neupert, W., and Schwarz, E. (1995) EMBO J. 14, 3452-3460[Abstract]
  43. Westermann, B., Gaume, B., Herrmann, J. M., Neupert, W., and Schwarz, E. (1996) Mol. Cell. Biol. 16, 7063-7071[Abstract]
  44. Herrmann, J. M., Fölsch, H., Neupert, W., and Stuart, R. A. (1994) in Cell Biology: A Laboratory Handbook (Celis, D. E., ed), pp. 538-544, Academic Press, San Diego
  45. Zinser, E., and Daum, G. (1995) Yeast 11, 493-536[Medline] [Order article via Infotrieve]
  46. Söllner, T., Rassow, J., and Pfanner, N. (1991) Methods Cell Biol. 34, 345-358[Medline] [Order article via Infotrieve]
  47. Segui-Real, B., Kispal, G., Lill, R., and Neupert, W. (1993) EMBO J. 12, 2211-2218[Abstract]
  48. Ou, W. J., Okazaki, H., and Omura, T. (1989) EMBO J. 8, 2605-2612[Abstract]
  49. Omura, T., and Ito, A. (1991) Methods Enzymol. 206, 75-81[Medline] [Order article via Infotrieve]
  50. Rep, M., van Dijl, M., Suda, K., Schatz, G., Grivell, L. A., and Suzuki, C. K. (1996) Science 274, 103-106[Abstract/Free Full Text]
  51. Prip-Buus, C., Westermann, B., Schmitt, M., Langer, T., Neupert, W., and Schwarz, E. (1996) FEBS Lett. 380, 142-146[CrossRef][Medline] [Order article via Infotrieve]
  52. Schneider, H. C., Westermann, B., Neupert, W., and Brunner, M. (1996) EMBO J. 15, 5796-5803[Abstract]
  53. Dekker, P. J., and Pfanner, N. (1997) J. Mol. Biol. 270, 321-327[CrossRef][Medline] [Order article via Infotrieve]
  54. Miao, B., Davis, J. E., and Craig, E. A. (1997) J. Mol. Biol. 265, 541-552[CrossRef][Medline] [Order article via Infotrieve]
  55. Rojo, E. E., Stuart, R. A., and Neupert, W. (1995) EMBO J. 14, 3445-3451[Abstract]
  56. Ou, W. J., Ito, A., Morohashi, K., Fujii-Kuriyama, Y., and Omura, T. (1986) J. Biochem. (Tokyo) 100, 1287-1296[Abstract]
  57. Usanov, S. A., Chernogolov, A. A., and Chashchin, V. L. (1990) FEBS Lett. 275, 33-35[CrossRef][Medline] [Order article via Infotrieve]
  58. Stuart, R. A., Cyr, D. M., Craig, E. A., and Neupert, W. (1994) Trends Biochem. Sci. 19, 87-92[CrossRef][Medline] [Order article via Infotrieve]
  59. Langer, T., and Neupert, W. (1994) in The Biology of Heat Shock Proteins and Molecular Chaperones (Morimoto, R. I., Tissières, A., and Georgopoulos, C., eds), pp. 53-83, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
  60. Voos, W., Gambill, B. D., Laloraya, S., Ang, D., Craig, E. A., and Pfanner, E. A. (1994) Mol. Cell. Biol. 14, 6627-6634[Abstract]
  61. Palleros, D. R., Reid, K. L., Shi, L., Welch, W. J., and Fink, A. L. (1993) Nature 365, 664-666[CrossRef][Medline] [Order article via Infotrieve]
  62. Szabo, A., Langer, T., Schröder, H., Flanagan, J., Bukau, B., and Hartl, F. U. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 10345-10349[Abstract/Free Full Text]
  63. Theyssen, H., Schuster, H. P., Packschies, L., Bukau, B., and Reinstein, J. (1996) J. Mol. Biol. 263, 657-670[CrossRef][Medline] [Order article via Infotrieve]
  64. Tomoyasu, T., Gamer, J., Bukau, B., Kanemori, M., Mori, H., Rutman, A. J., Oppenheim, A. B., Yura, T., Yamanaka, K., Niki, H., Hiraga, S., and Ogura, T. (1995) EMBO J. 14, 2551-2560[Abstract]
  65. Herman, C., Thevenet, D., D'Ari, R., and Bouloc, P. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 3516-3520[Abstract]
  66. Blaszczak, A., Zylicz, M., Georgopoulos, C., and Liberek, K. (1995) EMBO J. 14, 5085-5093[Abstract]
  67. Gamer, J., Multhaup, G., Tomoyasu, T., McCarty, J. S., Rüdiger, S., Schönfeld, H. J., Schirra, C., Bujard, H., and Bukau, B. (1996) EMBO J. 15, 607-617[Abstract]
  68. Rep, M., Nooy, J., Guélin, E., and Grivell, L. A. (1996) Curr. Genet. 30, 206-211[CrossRef][Medline] [Order article via Infotrieve]
  69. Maurizi, M. R. (1987) J. Biol. Chem. 262, 2696-2703[Abstract/Free Full Text]
  70. Van Dyck, L., Neupert, W., and Langer, T. (1998) Genes Dev. 12, 1515-1524[Abstract/Free Full Text]


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