From the School of Biochemistry & Molecular Genetics, Cooperative Research Centre for Food Industry Innovation, University of New South Wales, Sydney NSW 2052, Australia
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ABSTRACT |
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This study shows differences at the level of cell
cycle arrest between the response of yeast cells to hydrogen peroxide
and superoxide stress. These include both cell cycle phases at which arrest occurs and the involvement of the RAD9 checkpoint
gene. Wild-type and rad9 cells were treated with hydrogen
peroxide or the superoxide-generating agent menadione. rad9
mutants were up to 100-fold more sensitive to hydrogen peroxide but not
affected in their resistance to menadione. Hydrogen peroxide caused
G2-phase arrest, whereas menadione-treated cells arrested
in G1. G2 arrest, induced by methyl
2-benzimidazil carbamate, increased cellular resistance to hydrogen
peroxide but not to menadione. G1 arrest mediated by
-factor caused an increase in survival of wild-type cells treated
with menadione but not with hydrogen peroxide. A cdc28
mutant arrested in G1 was significantly more sensitive to hydrogen peroxide than other cdc mutants arrested in later
phases, including G2. rad9 cells have normal
stationary phase resistance to hydrogen peroxide, the ability to adapt
to it, glutathione content and induction of genes via the stress
responsive element. Although rad9-dependent
G2 arrest is important, other
rad9-dependent factors may be involved in the
resistance of cells to hydrogen peroxide since arrest in G2
did not make rad9 cells fully resistant.
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INTRODUCTION |
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Oxidative stress generated by hydrogen peroxide or the superoxide anion causes an adaptive response in the yeast, Saccharomyces cerevisiae. Cells pretreated with a low dose of these reactive oxygen species (ROS)1 subsequently adapt to become more resistant to higher doses (1-3). Study of the mechanisms that lie behind this response has focused mainly on the role of antioxidant enzymes and detoxifying molecules such as glutathione.
Several genes whose products either directly eliminate ROS or form part
of the glutathione synthesis pathway are transcriptionally regulated by
oxidative stress, e.g. CTT1 encoding cytosolic catalase, SOD1 and SOD2 encoding Cu, Zn- and Mn-superoxide
dismutase respectively, GSH1 encoding -glutamylcysteine
synthetase, GLR1 encoding glutathione reductase,
TRX2 encoding thioredoxin (a scavenger of ROS), and SSA1 encoding a stress-inducible heat shock protein (4-9).
Transcription factors and their target DNA sequences have been
identified in genes that respond to oxidative stress: Msn2p and Msn4p
function via the stress responsive element (STRE) (10), and the Yap1p binding site resembles the SV40 AP-1 binding sequence (8, 11). These
factors clearly play an important role in the stress response of yeast
cells since S. cerevisiae mutants deleted for
MSN2/4 or YAP1 are hypersensitive to various
types of stress including treatment with hydrogen peroxide.
Despite the fact that oxidative stress can cause damage to many
cellular components including proteins, lipids, and DNA, few studies
have considered the potential importance of cell cycle arrest in
response to DNA damage caused by hydrogen peroxide or superoxide. Both
prokaryotic and eukaryotic cells respond to DNA damage (caused by UV,
x-, or -irradiation) by delaying cell cycle progression to allow
repair of damage as well as preventing segregation of affected
chromosomes. In Escherichia coli, the SOS system controls cell cycle arrest and coordinates the activation of approximately 20 genes including those involved in nucleotide excision repair, recombination and mutagenesis (12, 13). An analogous system is thought
to exist in S. cerevisiae, although the precise mechanism is
not well understood.
Checkpoints can be defined as negative controls that cause cell cycle arrest in response to cellular damage. RAD9 is the best characterized of the S. cerevisiae checkpoint genes and has been shown to act at G1, S and G2 phases (14-16). The molecular function of RAD9 is unknown but cells that are defective in this gene exhibit increased sensitivity to DNA damaging agents. Treatment with the microtubule inhibitor methyl 2-benzimidazil carbamate (MBC), which blocks cells in the G2 phase, reversed this sensitivity indicating that RAD9 functions in cell cycle arrest and is not a DNA repair enzyme (16, 17). Curiously, however, MBC treatment did not rescue rad9 cells exposed to UV radiation, and this pointed toward a further function for RAD9 other than cell cycle arrest. Aboussekhra et al. (17) have recently shown that RAD9 is involved in transcriptional induction of genes responsible for multiple DNA metabolism/repair pathways, and postulated that the response pathways controlled by RAD9 may be the functional equivalents of the E. coli SOS response.
Neither hydrogen peroxide nor the superoxide radical can directly attack DNA (18), but DNA strand breaks arise when cells are exposed to these reagents due to the production of the highly reactive hydroxyl radical (19, 20). The relative importance of the repair of this damage in the general response of yeast cells to oxidative stress remains unclear. However, slow proliferation of a S. cerevisiae mutant strain lacking Cu/Zn superoxide dismutase (sod1) was due to a 2-fold increase in time spent in G1 phase (21). Under 100% O2 the mutant permanently arrested in G1 due to inhibition of transcription of the autoregulated G1 cyclins, CLN1 and CLN2. Moreover, treatment with paraquat, a superoxide-generating compound, resulted in pronounced G1 arrest in G1-synchronized yeast cells (22). Interestingly, cell cycle arrest under these circumstances was found to be independent of the RAD9 gene.
This study investigated the role of RAD9 cell cycle arrest
in the response to hydrogen peroxide and the superoxide-generating agent, menadione. These compounds have been shown to elicit overlapping but different responses in S. cerevisiae (2, 3, 23). We have
shown that a rad9 mutation rendered cells 10-100-fold more sensitive to hydrogen peroxide but, surprisingly, did not affect resistance to menadione. Since RAD9 is involved in
G1, S, and G2 arrest in response to DNA damage,
arrest induced by MBC and the mating pheromone, -factor, as well as
cell cycle arrest in temperature-sensitive cdc mutants was
used to investigate which checkpoint might be involved. Overall
capacity of the rad9 mutant to resist oxidative stress was
also studied by assessing resistance in stationary phase, glutathione
levels, ability to mount an adaptive response, and transcriptional
activation through STRE elements. Our results show that the
RAD9 function defines a fundamental difference between the
response to peroxide and superoxide stress. In addition, the acute
sensitivity of the rad9 mutant to hydrogen peroxide, which
is not fully rescued by MBC, indicates that a further
rad9-dependent function as well as G2 checkpoint
arrest may be important for survival during hydrogen peroxide
stress.
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MATERIALS AND METHODS |
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Strains and Media--
Wild-type yeast strain 7830-2-4a
MATa his3 leu2 trp1 ura3 and its isogenic mutant
7833-1a MATa his3 leu2 trp1 ura3
rad9::TRP1 were a gift from L. Hartwell; Y382
MAT ura3 leu2 ade2 ade3 trp1 used for flow cytometry was
obtained from A. Bender; and strains carrying mutations in CDC4,
CDC13, CDC16 and CDC28 each backcrossed several times
to wild-type MATa ade2-1 trp1-1 can1-100 leu2-3
leu2-112 his3-11 his3-15 ura3 GAL psi+ or its MAT
counterpart were obtained from the Research Institute of Molecular
Pathology, Vienna.
Hydrogen Peroxide/Menadione Treatments-- Cells were grown overnight to an A600 of 1 at 30 °C with shaking in the appropriate medium. Temperature-sensitive mutants were grown at 23 °C overnight and shifted to 37 °C for 4 h before treatment. Hydrogen peroxide or menadione was added directly to the medium when cells were treated in either YEPD or SD medium. When treatments were carried out in buffer, cells were harvested by centrifugation at 25 °C (4 × 103 × g for 5 min), washed, and resuspended in an equal volume of 100 mM potassium Pi buffer, pH 7.4. Samples (5 ml) were treated with hydrogen peroxide or menadione as indicated. In all cases, appropriate dilutions were made before plating cells on YEPD agar plates to obtain viable counts.
YEPD Agar Plate Test of Resistance-- Cultures were grown to stationary phase and diluted to an A600 of 3 and 0.3. Drops (4 µl) of each culture were placed onto YEPD agar containing appropriate concentrations of hydrogen peroxide, tert-butyl peroxide or menadione. Plates were dried, incubated at 30 °C for 2 days, and photographed.
Measurement of DNA Content-- Cells were fixed in 70% cold ethanol and stored overnight at 4 °C. Samples (10 ml) were washed and resuspended in 1 ml of 50 mM sodium citrate buffer, pH 7.5. RNase (25 µl of 10 mg/ml solution) was added, and the mixture was incubated for 1 h at 50 °C before addition of 50 µl of proteinase K followed by further incubation for 1 h at 50 °C. Propidium iodide (50 µg) in 50 mM sodium citrate buffer (1 ml) was added and samples were incubated in the dark at 4 °C overnight. Flow cytometry analysis of at least 100,000 cells was carried out for each sample in a MoFlo analyzer (Cytomation).
Cell Cycle Arrest and Treatment--
Cells were grown overnight
in the appropriate medium to an A600 of 0.5 at
30 °C with shaking, divided, and exposed to either -factor or
MBC.
-factor was purchased from Sigma and added at a final
concentration of 0.5 µg/ml. Stock MBC, purchased from Aldrich, was
prepared in a 10 mg/ml solution in Me2SO and added to cells
at a final concentration of 100 µg/ml. An equal volume of
Me2SO (without MBC) was added to the MBC treatment control culture. All cultures were incubated at 30 °C for one generation after which treatments were carried out as indicated above in the
presence of either
-factor or MBC, and cell survival was monitored
as described previously.
Adaptation to Hydrogen Peroxide-- Overnight cultures grown in YEPD to an A600 of 0.5 at 30 °C were exposed to hydrogen peroxide pretreatment doses at the concentrations indicated. Incubation at 30 °C was continued for a 1-h period after which cells were harvested, washed, resuspended in 100 mM phosphate buffer, and challenged with 1 mM hydrogen peroxide for 1 h. Cell survival was monitored as described previously.
Glutathione Assay-- Cultures were grown overnight to an A600 of 1, centrifuged, washed, and resuspended in ice-cold 8 mM HCl, 1.3% 5-sulfosalicylic acid. Cells were broken with glass beads in a mini-bead beater set at high speed for 40 s, microcentrifuged 15 min at 4 °C and the supernatant was collected. To measure total glutathione content, i.e. reduced (GSH) and oxidized (GSSG) glutathione, samples were diluted appropriately and added to buffer (143 mM sodium Pi, 6.3 mM EDTA, pH 7.4) containing 0.73 mM 5,5'-dithiobis(nitrobenzoic acid), 0.24 mM NADPH, 0.09% 5-sulfosalicylic acid. Reaction of 5,5'-dithiobis(nitrobenzoic acid) with GSH present in the sample led to formation of GSSG. This GSSG as well as that already present in the sample was measured by addition of 1.2 IU/ml GSSG-reductase and monitoring formation of 2-nitro-5-thiobenzoate from 5,5'-dithiobis(nitrobenzoic acid) over a 2 min period at 405 nm using a Molecular Devices Spectramax 340 microtiter reader.
To measure only GSSG, in a separate reaction the GSH present in the sample was derivatized by adding 5 µl of 2-vinylpyridine to a 100-µl sample and shaking 1 h at room temperature prior to measuring GSSG content as above. Subtraction of the amount of GSSG from the total glutathione allowed an estimation of GSH levels present in the sample.Plasmid Transformation--
Plasmid
7xSTRE::lacZ is described in Marchler et
al. (4). It includes seven copies of a synthetic STRE element
5'-GGTAAGGGGCCTTACC-3' in orientation cloned
upstream of a LEU2-lacZ fusion gene in vector pLS9 (4). The
construct was linearized by NcoI cleavage within the
URA3 gene prior to transformation into strains 7830-2-4a and
7833-1a using the lithium acetate method of Geitz et al.
(24). Single copy integration was checked by Southern blot analysis (data not shown) using the lacZ gene sequence as a
probe.
-Galactosidase Assays--
Assays for
-galactosidase
activity using o-nitrophenyl-
-D-galactose as
substrate were performed as described by Rose and Botstein (25).
Specific activity is expressed as nanomoles of o-nitrophenyl-
-D-galactose hydrolyzed × min
1 mg
1 protein. Protein concentration was
measured by the Bio-Rad assay method as indicated by the
manufacturer.
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RESULTS |
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Effect of a Cell Cycle Checkpoint Mutation on Hydrogen Peroxide and Menadione Resistance-- Cells that are sensitive to DNA damage may also be more sensitive to peroxide or menadione stress even if other factors involved in the oxidative stress response are intact. Cells defective in the RAD9 checkpoint gene are sensitive to radiation due to their failure to induce cell cycle arrest in response to DNA damage. To determine whether this arrest malfunction would render cells more sensitive to oxidative stress, a wild-type strain and its isogenic rad9 derivative were grown to an A600 of 1 in YEPD medium and treated with various concentrations of hydrogen peroxide over a 2-h period. rad9 cells were 10-100 times more sensitive than the wild-type at concentrations of hydrogen peroxide above 1 mM (Fig. 1). At concentrations of 2 mM or more, rad9 cell samples taken after 2 h of exposure showed less than 0.001% survival.
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Cell Cycle Arrest in Response to Reactive Oxygen Species-- Since rad9 cells fail to arrest either in G1, S, or G2 phase, it was important to assess at which stage of arrest cells acquired resistance to hydrogen peroxide. In addition, since paraquat (also a superoxide generator) induces G1 arrest (22), it was of interest to know whether menadione could do so.
Survival of menadione- or peroxide-treated cells in defined SD medium was intermediate between survival in YEPD and buffer (results not shown). This medium was therefore used for both hydrogen peroxide and menadione treatments to allow normal cell cycle progression upon exposure to low doses of the reagents and enable comparison between the treatments. Cells were grown to A600 of 1 and the culture split into three. Over a 3-h period, one aliquot was allowed to grow without treatment (control) and the second and third aliquots were treated with hydrogen peroxide or menadione, respectively. The proportion of cells in G1 or G2 phase was inferred from flow cytometry analysis in which DNA content of cells was measured (Fig. 4). Menadione, like paraquat, caused arrest in G1, and since rad9 cells are not particularly sensitive to menadione, this arrest is relatively independent of RAD9 function. Hydrogen peroxide treatment, however, caused cells to arrest in G2, which appears therefore to be a RAD9-dependent effect.
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Effect of G1 and G2 Arrest on Resistance to
Hydrogen Peroxide and Menadione--
Since hydrogen peroxide and
menadione treatment cause cells to arrest in G2 and
G1, respectively, it was hypothesized that agents causing
cell cycle arrest in the appropriate phase prior to oxidative stress
treatment would confer resistance to each particular reagent. Wild-type
and rad9 cells in exponential phase (A600 of 0.5) were treated with either
-factor or MBC, which arrest cells in G1 and
G2/M phase, respectively (26, 27). Cultures were incubated
for a further generation time (3 h) and arrest was confirmed by
microscopic observation before treatment with various concentrations of
either hydrogen peroxide or menadione. To take into account differences
in sensitivity to hydrogen peroxide treatment of the wild-type and
rad9 strains, the experiment was conducted such that
comparison could be made between treatments, which resulted in similar
survival levels (2 mM hydrogen peroxide for the wild-type
and 1 mM hydrogen peroxide for rad9 cells) and similar treatment concentrations (i.e. 2 mM
treatments for both cell types). Survival was calculated as a
percentage of cells present before treatment with the ROS.
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Hydrogen Peroxide Resistance of Temperature-sensitive Cell Division Cycle (cdc) Mutants-- Many temperature-sensitive mutants have been isolated that arrest at defined points in the cell cycle when shifted to the restrictive temperature. A range of mutants was examined to determine the effect of each mutation on resistance to hydrogen peroxide. Strains tested included cdc28 mutants, which cause arrest at START, the control point at which cells leave G1 and enter the mitotic cycle (28, 29), cdc4, which arrests after START but prior to separation of the spindle pole bodies (28, 30), and cdc13 and cdc16, which both cause arrest in G2/M phase (31).
Wild-type and mutant cells were grown overnight at 23 °C and shifted to the restrictive temperature 4 h prior to treatment in the YEPD growth medium. All strains were significantly less sensitive to hydrogen peroxide at the restrictive temperature than at 23 °C (results not shown), which may have been due to the heat shock experienced. Arrest in G1 (cdc28 mutant) caused cells to be particularly sensitive to hydrogen peroxide, the least sensitive were the cdc13 and cdc16 mutants blocked in G2/M (Fig. 6), which would be expected if G2 arrest was needed to repair peroxide damage. Curiously, none of the arrested cdc mutants became more resistant than the wild-type.
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Hydrogen Peroxide Resistance of Stationary Phase rad9 Cells-- Mutational analysis has shown that stationary-phase cells are in a unique state and are not arrested at a point in the mitotic cell cycle (32). Stationary phase cells are more resistant to several types of stress including oxidative stress (2, 33). If the rad9 sensitivity to hydrogen peroxide is due to its dysfunctional checkpoint arrest then in stationary phase when cells are not undergoing cell division, their resistance to hydrogen peroxide should increase.
The rad9 and wild-type cells were grown to stationary phase in YEPD (a 3-day growth) and treated for 1 h with various concentrations of hydrogen peroxide (Fig. 7). Comparison with data in Fig. 1 (exponential phase cells) reveals that, as shown by others, survival of the wild-type was substantially higher in cells grown to stationary phase. Of more interest, however, is that rad9 cells not only increased their survival rate but became as resistant to hydrogen peroxide as wild-type cells (4 mM hydrogen peroxide treatment killed more than 99.9% of exponential phase rad9 cells but less than 50% of stationary phase cells). The RAD9 function, therefore, does not play a role in the increased resistance of stationary phase cells to oxidative stress.
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Assessment of Antioxidant Defense Systems in rad9 Cells-- G2/M arrest by MBC significantly increased survival of rad9 cells but did not restore wild-type survival levels. RAD9 has recently been shown to control not only the cell cycle checkpoint delay but also DNA damage-dependent transcriptional induction of a "regulon" of genes whose products are involved in several DNA repair pathways. This secondary control appears to be independent of the cell cycle (17). It was important therefore to determine whether RAD9 also played a role in induction of the overall response to oxidative stress and whether the increased sensitivity of rad9 cells to hydrogen peroxide treatment reflected an inability to activate the correct stress response in addition to the inability to arrest at the RAD9 checkpoint. Therefore, rad9 cells were assessed for their level of the important antioxidant glutathione; their ability to show an adaptive response to hydrogen peroxide; and induction of transcription through the STRE element.
Glutathione, an ubiquitous thiol which acts as a scavenger of free radicals, is essential for resistance and adaptation to hydrogen peroxide (34, 35). It is the most abundant antioxidant molecule (36) in the cell and was thus a good candidate for investigation of the intrinsic ability of rad9 cells to eliminate free radicals prior to them reaching the nucleus. Moreover, the ratio of reduced to oxidized glutathione is an indication of the capacity of cells to deal with oxidative stress. Glutathione content of exponential phase wild-type and rad9 cells was analyzed in YEPD-grown cultures. Both oxidized and reduced glutathione levels in wild-type (12 ± 2 and 559 ± 95 nmol/109 cells, respectively) and rad9 (13 ± 3 and 473 ± 77 nmol/109 cells, respectively) were similar and glutathione content, therefore, appears to be unrelated to RAD9. The adaptive response to oxidants includes transcriptional induction of genes encoding protective proteins. There are some difficulties interpreting the results when assessing whether rad9 cells were impaired in this protective mechanism due to the large differences in sensitivity to hydrogen peroxide compared with wild-type cells. It was estimated from kill curve data that a 0.1 mM pretreatment of wild-type cells may be equivalent to a 0.05 mM pretreatment of rad9 cells. Pretreatments were carried out for 1 h in YEPD, and concentrations varied from 0.05 to 0.2 mM hydrogen peroxide while the challenge treatment (1 mM for 1 h in 100 mM Pi buffer) was kept constant (Fig. 8). Despite different sensitivities of the cell types, the magnitude of the adaptive response was comparable when pretreatments were equivalent i.e. a 3.5-fold increase in survival for both strains upon pretreatment with 0.1 mM peroxide; 6.4- and 9.5-fold survival increase for wild-type and rad9 strains, respectively, when pretreated with 0.2 mM peroxide. It was concluded therefore that there was no impairment of the adaptive response per se in rad9 cells despite their increased sensitivity to hydrogen peroxide.
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DISCUSSION |
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In addition to their UV, x-, and -radiation sensitivity, this
study has demonstrated that cells defective in RAD9 are also extremely sensitive to hydrogen peroxide compared with wild-type cells.
RAD9 checkpoint arrest in response to DNA damage operates in
G1, S, and G2 phases, although the mechanism by
which it does so is not entirely clear (14-16). Flow cytometry
analyses revealed that hydrogen peroxide arrests cells in
G2 phase, and it is suggested, therefore, that this is at
least partly due to the cell cycle arrest function of
RAD9.
Hydrogen peroxide does not directly damage DNA but forms the hydroxyl radical that can then attack bases, sugars, and the phosphate backbone of the DNA helix (13). It has been estimated that approximately 65% of DNA damage caused by ionizing radiation is also due to hydroxyl radical attack, whereas approximately 35% is due to direct ionization effects (37). Hence, it could be anticipated that the responses to hydrogen peroxide and ionizing radiation would overlap considerably.
MBC treatment can functionally substitute for G2 arrest and
rescue rad9 cells treated with x- and -radiation (16,
17). However, MBC treatment prior to hydrogen peroxide challenge only partially rescues rad9 cells. This may imply that whereas
ionizing radiation-sensitivity of rad9 cells is entirely due
to the cell cycle arrest function of RAD9, the
RAD9-dependent response to hydrogen peroxide relies on
another function of RAD9 other than G2 arrest.
Although RAD9 transcript is not induced by UV or
x-irradiation (38, 39) and transcriptional induction is not required
for G2 cell cycle arrest after DNA damage, RAD9
is responsible for increased expression of genes involved in DNA repair
pathways (17, 39). Interestingly, Aboussekhra et al. (17)
found that MBC treatment of rad9 cells prior to UV
irradiation, like hydrogen peroxide treatment, did not restore survival
to wild-type levels. They speculated, therefore, that RAD9
may play only a checkpoint role in x- and
-irradiation defense but
that it may control transcriptional activation as well as checkpoint
arrest in response to UV damage. This appears to be the case for the
response to hydrogen peroxide although the response to hydrogen
peroxide and UV radiation is also different (see later discussion).
Like hydrogen peroxide, the superoxide radical is not capable of causing DNA damage and is converted to the hydroxyl radical to do so (19, 20). In theory, therefore, the cellular response to DNA damage caused by menadione should be similar to the response to ionizing radiation- and hydrogen peroxide-induced damage. However, the rad9 mutation had little effect on resistance to menadione. This highlights a significant difference between the response of S. cerevisiae to peroxide and superoxide stress extending our previous observation that the adaptive response of S. cerevisiae to menadione and hydrogen peroxide differs (3).
Both superoxide stress in G1-arrested wild-type cells, and
dioxygen stress in a mutant unable to efficiently dispose of
superoxide, resulted in G1 arrest (21, 22). Our flow
cytometry results show that menadione arrests asynchronous cells in
G1 phase. In addition, arrest in G1 using
-factor caused an increase in wild-type resistance to menadione
treatment. Siede et al. (14) suggest that arrest by
-factor precedes the RAD9 checkpoint in G1
and that increased survival after UV irradiation due to cell cycle arrest is dependent on a process that operates between these two points. If menadione-induced arrest also occurs at this point it would
account for the resistance of rad9 cells to menadione as
well as the increased survival of pheromone-arrested wild-type cells.
Wild-type stationary phase cells are more resistant to hydrogen peroxide than exponential phase cells (9). This study has shown that increased resistance in stationary phase is independent of RAD9 function. Lee et al. (21) found that dioxygen-induced G1 arrest was due to inactivation of G1 cyclins and likened the arrest to a stationary phase state in which antioxidant defenses are activated at the expense of cell functions that promote growth and/or proliferation. For resistance to hydrogen peroxide, however, the effects of G1 arrest and stationary phase are opposite. This would support the theory that stationary phase cells are in a unique state which is "outside" the cell cycle (32) and not in a prolonged G1 phase.
Furthermore, the ability of rad9 cells to increase their resistance to hydrogen peroxide up to wild-type levels on entering stationary phase is interesting in light of the fact that neither wild-type nor rad9 cells in stationary phase are more resistant to UV than exponential phase cells; in fact, both cell types appear to be slightly more sensitive to UV radiation in stationary phase (17). Thus, the effect of stationary phase on wild-type and rad9 resistance to hydrogen peroxide and UV treatment emphasizes the fact that a different defense mechanism is put in place in response to these stresses despite their common requirement for a function of RAD9 other than cell cycle arrest.
Distinct differences in cell cycle arrest defense mechanisms depending on the source of oxidative stress have been noted in mammalian cell lines (see Ref. 40 for review). For example, cumene hydroperoxide causes delayed cell activation before onset of S phase and a shortening of G1 phase after the first mitosis in diploid skin fibroblasts. In amniotic fluid-derived fibroblasts, cumene hydroperoxide caused a prolongation of S and G2 phases in addition to delayed cell activation and shortening of the G1 phase after the first mitosis. The breakdown product of lipid peroxides, 4-hydroxynonenal, induces delayed activation and prolongation of G1 phase without affecting other cell cycle compartments. Cumene hydroperoxide and 4-hydroxynonenal, therefore, disturb cell cycle patterns in a manner that triggers different, but perhaps partially overlapping, cellular targets. However, human lymphoid B-cells exposed to 20 µM paraquat exhibit an entirely different pattern; there is a prolonged S phase, a shortened G1 phase, and cycling cells are arrested in the first G2 phase. It is possible, therefore, that S. cerevisiae has incorporated into its defense mechanisms specific responses to different forms of stress that include, like the mammalian response, cell cycle arrest at distinctly different stages.
CDC28 encodes a protein kinase essential for traversing
START, the point at which cells enter the cell cycle from the
G1 state (41). cdc28 temperature-sensitive
mutants shifted to the nonpermissive temperature were considerably more
sensitive to hydrogen peroxide treatment than wild-type. This is
consistent with the observation that arrest in G1 by
-factor also results in decreased resistance to hydrogen peroxide.
This phenomenon may be partly due to the fact that the asynchronous
wild-type culture already contains cells that are in, or close to, the
appropriate arrest point to resist hydrogen peroxide, whereas the
cdc28 culture, in which cells are arrested in G1
phase only, does not. Interestingly, mutations which resulted in arrest
at other stages of the cell cycle including G2/M did not
improve survival over wild-type levels. It may be that MBC (which
improves resistance to hydrogen peroxide) arrests cells at a specific
point in G2/M that does not correspond to any of the
mutations chosen for investigation. Alternatively, it could be that the
heat shock endured by the cells prior to treatment can elicit a
response in wild-type cells, which it is unable to do in the mutant
cell types.
S. cerevisiae mutants deficient in superoxide dismutase, catalase, or glutathione do not differ significantly in radiation sensitivity (42). However, since we concluded that factors other than the G2 cell cycle arrest function were partly responsible for the increased sensitivity of rad9 cells to hydrogen peroxide, it was of interest to assess whether indicators of the "classic" response to oxidative stress were compromised in the rad9 strain. All of the functions tested paralleled those of wild-type cells, thus, many of the features commonly associated with resistance to oxidative stress are intact in rad9 cells despite their striking sensitivity to hydrogen peroxide. The reason for the intrinsic susceptibility of rad9 cells to hydrogen peroxide as well as their ability to resist attack by menadione is still unclear.
In conclusion, given the acute sensitivity of the rad9 strain to hydrogen peroxide, cell cycle arrest in response to this oxidant may be as important as the ability to induce synthesis of the classic antioxidant defense molecules. Differences between the responses to UV, ionizing radiation, hydrogen peroxide, and menadione infer that part of the mechanism of cell cycle arrest in response to each of these stresses is very specific. In addition, since ionizing radiation, hydrogen peroxide, and superoxide cause DNA damage mainly by the same means, through formation of the hydroxyl radical, the question arises as to whether there may be some other trigger for cell cycle arrest in response to these stresses apart from DNA damage.
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ACKNOWLEDGEMENTS |
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We thank Fiona MacIver and Prerna Badhwar for technical assistance, Andrew Collins for help with fluorescence-activated cell sorter analyses, Lee Hartwell and Barbara Garvik for supplying the isogenic wild-type and rad9 knockout strains, and Alan Bender for strain Y382.
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FOOTNOTES |
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* This work was supported in part by a grant from the Australian Research Council.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Recipient of an Australian Postgraduate Research Award.
§ To whom correspondence should be addressed. Tel.: 61-2-9385-2089; Fax: 61-2-9385-1050; E-mail: i.dawes{at}unsw.edu.au.
1 The abbreviations used are: ROS, reactive oxygen species; STRE, stress responsive element; MBC, methyl 2-benzimidazil carbamate.
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REFERENCES |
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