From the Institute of Biochemistry, University of
Fribourg, 1700 Fribourg, Switzerland, the § Department of
Microbiology and Molecular Genetics, University of Medicine and
Dentistry-New Jersey Medical School, Newark, New Jersey 07107, the
¶ Department of Biochemistry, Medical College of Wisconsin,
Milwaukee, Wisconsin 53226, and the
Kitasato Institute,
Shirokane, Minato-ku, Tokyo 108, Japan
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ABSTRACT |
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Apoptosis requires the activation of caspases
(formerly interleukin 1-converting enzyme-like proteases), in
particular those related to the caspase-3/7/6 subfamily. Recent data,
however, revealed that, although caspase-specific inhibitors delay
apoptosis, they are often incapable of preventing it. To obtain
evidence for caspase-independent steps of apoptosis, we artificially
created a high amount of short-lived or aberrant proteins by blocking the ubiquitin degradation pathway. A temperature-sensitive defect in
the ubiquitin-activating enzyme E1 induced apoptosis independent of the
activation of caspase-3 and -6 and the cleavage of their respective
substrates poly(ADP-ribose) polymerase and lamin A. In addition,
neither the caspase 3/7-specific inhibitor
N-benzyloxycarbonyl-Asp-Glu-Val-Asp-fluoromethylketone nor the general caspase inhibitor
N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone were
capable of blocking this type of cell death. By contrast, Bcl-2
overexpression effectively protected cells from apoptosis induced by a
defect in the E1 enzyme at the nonpermissive temperature. Bcl-2 acted
downstream of the accumulation of short-lived or aberrant proteins
because it did not prevent the overexpression of the short-lived
proteins p53, p27kip1, and cyclins D1 and B1 under conditions
of decreased ubiquitination. These results suggest the existence of
short-lived proteins that may serve the role of caspase-independent
effectors of apoptosis and attractive targets of the death-protective
action of Bcl-2.
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INTRODUCTION |
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Programmed cell death (apoptosis) is an essential process to maintain homeostasis in multicellular organisms. It is triggered by a variety of physiological and nonphysiological agents that generate a common set of morphological alterations such as cell shrinkage, surface blebbing, chromatin condensation, DNA and nuclear fragmentations, and the formation of membrane-enclosed apoptotic bodies that are phagocytosed by neighboring cells (1).
The apoptotic process can be divided into three, functionally distinct
phases: initiation, effector, and degradation (2). The initiation phase
involves the activation of heterogeneous intracellular signaling
pathways that are distinct ("private") for each death stimulus.
These pathways converge on a common effector phase that, in turn,
executes death by degrading various cellular components (3). The
crucial molecular players in these phases are a series of cysteine
proteases that are homologues of the interleukin 1-converting enzyme
(4). Unlike other cysteine proteases, these enzymes cleave their
substrates following aspartate residues and have therefore been named
caspases (5). All caspases exist as zymogens that require cleavage at
internal aspartate residues to generate two-subunit active enzymes (6).
Because of this property, they can activate each other and form an
amplified protease cascade similar to that seen in clotting and
complement activation (6). Most caspases are organized in "private"
signaling pathways because their deletion or inhibition by specific
inhibitors (Ac-YVAD-CHO, cytokine response modifier A (CrmA)) does not
interfere with all sorts of apoptosis (7-9). However, at least three
caspases, caspase-3, -6, and -7, seem to participate in the common
death effector phase of apoptosis (5, 10-12). They are the closest homologues of the Caenorhabditis elegans ced-3 gene product
shown to be essential for apoptosis during nematodal development (13). Moreover, specific peptide inhibitors of caspase-3 and -7 such as
Z-DEVD-fmk1 interfere with
most, if not all, forms of mammalian apoptosis. This suggests that the
caspases of the death effector phase have been evolutionarily
conserved, but that higher organisms have adopted variations of these
enzymes to regulate "private" initiation phases as well. Numerous
substrates of caspase-3, -7, and -6 have been identified in mammalian
cells. Among them are poly(ADP-ribose) polymerase (PARP), a repair
enzyme preferentially cleaved by caspase-3 and -7 (14), and lamin A, a
component of the nuclear lamina, preferentially cleaved by caspase-6
(15, 16).
Genetic studies in C. elegans further uncovered a gene product called ced-9, which has an inhibitory effect on apoptosis during nematodal development (13). The mammalian homologue of ced-9 is Bcl-2, a proto-oncogene product that was originally identified at a t(14;18) chromosomal breakpoint common to human follicular lymphomas (17). Bcl-2 can functionally substitute for ced-9 and prevent nematodal (17) as well as mammalian apoptosis induced by various, often unrelated death stimuli (18). Both, genetic and biochemical studies revealed that ced-9 and Bcl-2 act upstream of ced-3 and caspase 3, respectively, to block caspase activation and apoptosis (13, 19-21). However, neither ced-9 nor Bcl-2 directly interact with their respective caspases. The link has been provided by ced-4, another gene product essential for apoptosis in C. elegans (13). ced-4 directly binds to and activates ced-3/caspases and triggers nematodal and mammalian apoptosis in a ced-3-dependent manner (13, 22, 23). Simultaneously, ced-4 interacts with ced-9/Bcl-2 and allows the latter to block the activation of ced-3/caspases (22-24). Thus, ced-4 has a double impact on the effector phase of apoptosis; it can act as a caspase activator and as an adapter for Bcl-2 to inhibit caspases. A further characterization of the function of ced-4 has recently become available through the cloning of a mammalian homologue, called Apaf-1 (25).
On the search for mammalian activators of caspases such as ced-4 homologues, we made the following assumption. If these activators were constitutively expressed, as it had been proposed (26), their death-promoting activities should be dormant in surviving cells. Any death stimulus would have to reactivate them by posttranslational modifications or the binding or release of regulatory proteins. An attractive, but often overlooked alternative is changes in the protein half-lives of the caspase activators. Since the ubiquitin system is a prominent regulator of protein turnover, we tested whether defects in this system would lead to caspase activation or apoptosis.
Ubiquitin-dependent degradation is necessary to eliminate damaged, misfolded proteins as well as proteins whose impact on biologic processes has to be of short duration (short-lived proteins) (27). In this pathway, ubiquitin (Ub) is first activated in an ATP-dependent manner by a thiolester linkage to the Ub-activating enzyme, E1. Activated Ub is then transferred to one of several Ub-conjugating enzymes, E2, to form another thiolester intermediate. From E2, Ub is ligated to lysine residues of target substrates sometimes with the help of specific E3 Ub protein ligases. Further ubiquitination of the mono-Ub protein substrates leads to the formation of multi-Ub chains that are recognized by the 26 S proteasome for degradation. An essential protease of the 26 S proteasome has recently been found to have a threonine residue in its active center. This site has subsequently been identified as the target for the specific proteasomal inhibitor lactacystin (28). While several E2 enzymes exist, only one E1 enzyme has been found thus far in mammalian cells (27). Thus, inactivation of the E1 enzyme abrogates ubiquitination and leads to an accumulation of aberrant or short-lived protein (29). This is accentuated by a further blockage of the proteasome by lactacystin.
We show here by using a cellular system of diminished E1 activity and simultaneous proteasomal inhibition that the turnover of short-lived proteins plays a crucial role in the apoptotic response. When present in high amounts, these proteins seem to activate a caspase-independent type of apoptosis that is still effectively impeded by Bcl-2 overexpression.
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MATERIALS AND METHODS |
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Reagents-- Hygromycin B, cycloheximide (CHX), Hoechst 33342, and Z-DEVD-fmk were purchased from Juro Supply/Calbiochem and DNase-free RNase from Boehringer Mannheim. Polyvinylidene difluoride (PVDF) membranes (Immobilon-P) was from Millipore, and the enhanced chemiluminescence (ECL) detection system was from Amersham. Z-VAD-fmk was from Enzyme System Prod., and MG132 was provided by Proscript Inc. The LIVE/DEAD fluorometric assay was purchased from Molecular Probes.
Cells-- The temperature-sensitive ts20 Balb/C 3T3 clone A31 fibroblast cell line and its E1-corrected H38-5 derivative were cultured as described previously (29). Vector control (pMV12) and Bcl-2-overexpressing (Bcl-2) ts20 cells were generated by retroviral transduction of the pMV12hygro plasmid lacking or containing the murine Bcl-2 cDNA. Construction of the viruses was exactly as described (30). Following infection, cells were selected on 200 µg/ml hygromycin for 10 days. Resistant clones were picked, expanded into cell lines, and analyzed for Bcl-2 expression by anti-Bcl-2 Western blot analyses. ts20Bcl-2#7 and ts20Bcl-2#13 expressing high and intermediate levels of murine Bcl-2, respectively, as well as the vector control cell line ts20pMV12, were used for further analyses. Other clones were tested as well and showed similar results. All ts20 cell derivatives were grown in Dulbecco's modified Eagle's medium containing 10% fetal calf serum and low amounts (50 µg/ml) of hygromycin to maintain Bcl-2 expression. Where indicated, specific drugs were directly added to the culture medium to induce apoptosis.
Analysis of Cell Proliferation and Viability-- Cells were seeded at a density of 1 × 105 cells/60-mm plate in 4 ml of Dulbecco's modified Eagle's medium plus 10% fetal calf serum (without hygromycin). They were left untreated or treated with various drugs, kept at 34 °C, or shifted to 39 °C to inactivate the E1 enzyme. Cells were counted on triplicate plates with media changes every second day. Cell viability was determined by trypan blue exclusion or the LIVE/DEAD fluorometric assay as described by the manufacturer (Molecular Probes).
DNA/Nuclear Fragmentation and Chromatin Condensation Assays-- To determine the degradation of chromosomal DNA into nucleosome-sized fragments, cells were trypsinized for 5 min and washed twice in 20 mM Tris, pH 8.0. DNA was immediately extracted by phenol/chloroform/isoamyl alcohol (25:24:1) and treated with 100 µg/ml RNase at 37 °C for 30 min to eliminate RNA. The pure DNA fraction was loaded onto a 2% agarose gel, electrophoresed, and visualized by ethidium bromide staining.
Chromatin condensation and nuclear fragmentation was assayed by Hoechst 33342 staining. The cells were grown on 12-mm glass coverslips and kept at 34 °C or shifted to 39 °C in the presence or absence of various drugs. At various time points, the cells were fixed in 4% paraformaldehyde containing 4 µg/ml Hoechst 33342 and incubated at 34 °C for 30 min. The stained nuclei were visualized under a Zeiss Axiovert microscope (magnification, ×1000) using a UV filter in the range of 355-465 nm.Cell Cycle Analysis by Flow Cytometry-- A recently developed DNA extraction method was used to simultaneously determine fragmented DNA (apoptotic cells) and DNA ploidy (cell cycle phases) (31). Briefly, cells were detached from the plate, fixed in 70% ethanol (v/v) on ice, rinsed once in phosphate-buffered saline, and incubated in DNA extraction buffer (0.2 M Na2HPO4, pH 7.8, 4 mM citric acid) for 5 min. After centrifugation, the cell pellet was suspended in DNA staining solution (10 mM Pipes, pH 6.8, 2 mM MgCl2, 100 mM NaCl, 0.1% (v/v) Triton X-100) containing 1 µg/ml 4',6'-diamidino-2-phenylindole (DAPI, Molecular Probes) and analyzed by flow cytometry using UV light at excitation 351 nm (argon ion line) and blue DAPI emission at 460-500 nm.
Protein Extraction/Western Blots--
Total cellular extracts
were prepared as follows; ts20 cell derivatives were washed three times
in phosphate-buffered saline and then lysed in H8 (20 mM
Tris-HCl, pH 7.5, 2 mM EDTA, 2 mM EGTA, 6 mM -mercaptoethanol, 10 µg/ml aprotinin, 2 µg/ml
leupeptin) containing 1% SDS, followed by sonication. Protein was
determined by the method of Lowry. Equal amounts of protein were loaded
onto 8-12% polyacrylamide gels, subjected to SDS-PAGE, and
transferred to a PVDF membrane as described (30). The following primary antibodies were used: rabbit polyclonal anti-mouse/rat Bcl-2 (27-6, 1:5000, Ref. 30); rabbit polyclonal anti-ubiquitin (1:1000, Ref. 32);
mouse monoclonal anti-p53 (Ab240, 1:2, Ref. 33) kindly provided by
Richard Iggo, ISREC, Lausanne, Switzerland; rabbit polyclonal
anti-caspase 3 p17 (1:10000, Ref. 34) kindly provided by Donald W. Nicholson, Merck Frosst, Quebec, Canada; rabbit polyclonal anti-PARP
(419, 1:2000, Ref. 35) kindly provided by Guy Poirier, Laval
University, Quebec, Canada; rabbit polyclonal anti-Bax (1:10000)
generously given by Le Sun, Upstate Biotechnology Inc., Lake Placid,
NY; mouse monoclonal anti-p27kip1 antibody (1:2500,
Transduction Laboratories); and goat polyclonal anti-Mch2 p20
(caspase-6), goat polyclonal anti-lamin A, rabbit polyclonal
anti-cyclin B1, and mouse monoclonal anti-cyclin D1 (all 1:1000,
Santa Cruz Biotechnology, Inc., Santa Cruz, CA). Secondary antibodies
were horseradish peroxidase-coupled goat anti-mouse and
anti-rabbit and donkey anti-goat antibodies (Jackson Laboratories). The detection system used was enhanced chemiluminescence (ECL).
Quantitation of Proteins-- Autoradiography of Western blots (ECL) was scanned with a Bio-Rad GS-700 densitometer, and the protein bands were quantified by imaging densitometry (Molecular Analyst® 2.1). Arbitrary integrated volume units (O.D. × area) were presented in the graphics. The values were corrected against the background of the autoradiography and the protein amount on the PVDF membrane determined by Amido Black staining.
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RESULTS |
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A Defect in the E1 Enzyme Leads to Growth Arrest-- ts20 is a derivative of Balb/c 3T3 fibroblasts that harbors a temperature-sensitive defect in the E1 ubiquitin-activating enzyme (29). At the nonpermissive temperature (39 °C), E1 enzyme activity is largely decreased, protein ubiquitination wanes and the cells are arrested at G1 or G2/M phases (see also Fig. 6). To rescue ts20 cells from the defects of ubiquitination and growth, H38-5 cells were generated by transfecting a wild-type allele of the E1 enzyme (29). At 34 °C, both H38-5 and ts20 cells displayed exponential growth characteristics over a time period of 4-7 days (Fig. 1A). The rate of proliferation was slower for H38-5, presumably due to a clonal effect (Fig. 1A). Upon shifting the temperature to 39 °C, H38-5 cells continued to proliferate while ts20 cells declined in number after an initial increase during the first 24 h (Fig. 1B).
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A Defect in the E1 Enzyme Induces Apoptosis-- The loss of ts20 cells at 39 °C was indicative of cell death in addition to growth arrest. Indeed, after 24 h at 39 °C, the ts20 cells (data not shown) and its vector control analog ts20pMV12 (Fig. 2A) exhibited morphological changes typical of apoptosis such as cell shrinkage and plasma membrane blebbing. In addition, the cells detached from the plate, stained with trypan blue, and displayed chromatin condensation, the fragmentation of nuclei (Fig. 2C), and the cleavage of genomic DNA into nucleosome-sized fragments (Fig. 2B). None of these changes were observed with ts20 and ts20pMV12 cells at 34 °C or H38-5 at both 34 °C and 39 °C at any time (Fig. 2 and data not shown). This indicates that a defect in the E1 enzyme provokes apoptosis.
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Overexpression of Bcl-2 Delays Apoptosis Due to a Defective E1 Enzyme-- To examine whether Bcl-2 interfered with apoptosis induced by a defective E1 enzyme, we infected ts20 cells with a retrovirus containing the murine Bcl-2 cDNA (ts20Bcl-2). In parallel, vector control cell lines were generated (ts20pMV12). Immunoblot analysis revealed various degrees of overexpression of the 26-kDa Bcl-2 in stable ts20Bcl-2 cell lines at both 34 °C and 39 °C (Fig. 1C). In addition, as shown by immunocytochemistry, the overexpressed Bcl-2 was correctly localized to mitochondrial, endoplasmic reticulum, and nuclear membranes (data not shown).
First, we measured the proliferation of ts20pMV12 and ts20Bcl-2 cells by counting viable (trypan-excluding) cells. At 34 °C, ts20Bcl-2#7 and ts20Bcl-2#13 cells proliferated slightly slower than the ts20pMV12 vector control cell line (Fig. 1A). As the parental cells, the vector control cells decreased in number after 24 h at 39 °C (Fig. 1B). On the other hand, both the ts20Bcl-2#7 and ts20Bcl-2#13 cell lines continued to proliferate at 39 °C although never as much as the E1-expressing H38-5 cells. Whereas the low Bcl-2-expressing cell line ts20Bcl-2#13 showed some cell loss after 48 h at 39 °C, this was not the case for the high Bcl-2-expressing ts20Bcl-2#7. Even after 7 days, the latter cells were still alive, although they did not further proliferate (Fig. 1B). At 48 h, no morphologic changes, chromatin condensation or nuclear or DNA fragmentation were seen in ts20Bcl-2#7 cells, whereas all these properties were evident in the ts20pMV12 vector control (Fig. 2). These data indicate that Bcl-2 partially rescues ts20 cells from growth inhibition and apoptosis at the nonpermissive temperature.Apoptosis Induced by a Defective E1 Enzyme Is Independent of Caspase Activation-- Next, we investigated the molecular mechanisms underlying apoptosis due to a defective E1 enzyme. For that purpose, we monitored the activation of caspase-3 and -6 and the cleavages of their respective substrates PARP and lamin A in ts20pMV12 cells at 39 °C for 72 h. Western blot analyses revealed that neither the 32-kDa proform of caspase-3 nor the 34-kDa proform of caspase-6 was converted into, respectively, active 17-kDa or 21-kDa species at any time during apoptosis of ts20pMV12 cells at 39 °C (Fig. 3, A and B, right panels). In addition, no cleavages of PARP (Fig. 3A) or lamin A (Fig. 3B) were detected. Since PARP is also a known substrate for caspase-7 (11), none of the effector caspases appeared to be activated during apoptosis due to a defect in the E1 enzyme. ts20pMV12 cells, however, displayed efficient maturation/activation of caspase-3 and caspase-6, as well as cleavages of PARP and lamin A upon apoptosis induced by staurosporine at 34 °C (data not shown) and 39 °C (Fig. 3, A and B, left panels), indicating that the cells were not deficient in activating the caspase execution program when exposed to appropriate apoptotic stimuli.
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Bcl-2 Neither Acts as E1 enzyme Nor Diminishes the Accumulation of Short-lived/Aberrant Protein at 39 °C-- To unravel how Bcl-2 protects ts20 cells from apoptosis induced by a defective E1 enzyme, we investigated the effect of Bcl-2 on the ubiquitin/proteasome degradation system. As shown by anti-ubiquitin Western blotting, a defect in E1 at 39 °C caused a marked diminution of overall protein ubiquitination, including that of histones and high molecular weight proteins (Fig. 5, A and B). Although this reduction was not complete due to residual E1 activity, it was sufficient to provoke the massive accumulation of short-lived proteins such as p53, the cyclin-dependent protein kinase (cdk) inhibitor p27kip1, and the cyclins D1 and B1 (Fig. 5, C, D, and F), all known targets of the ubiquitin/proteasome system (29, 36-39). While p53, p27kip1, and cyclin B1 control entry into the S and M phases, respectively, cyclin D1 primarily stimulates cell cycle progression during the G1 phase (36, 37, 40-42). Accumulation of these proteins perturbs cell cycle homeostasis and leads to cell cycle arrests or apoptosis at the G1/S (p53, p27kip1, and cyclin D1) or G2/M (cyclin B1) boundaries (42, 43). Interestingly, in both ts20Bcl-2#7 and ts20Bcl-2#13 cells, diminished ubiquitination was not restored to normal levels (Fig. 5, A and B). Moreover, p53, p27kip1, and the cyclins D1 and B1 all still accumulated to similar levels (Fig. 5, C, D, and F) as in ts20pMV12 cells during the 39 °C incubation. These results show that Bcl-2 cannot act as E1 enzyme to restore normal levels of ubiquitination during its death protective action. Furthermore, as exemplified by the accumulation of four different short-lived proteins, Bcl-2 cannot target these proteins to an ubiquitin-independent degradation pathway such as a direct degradation by the proteasome or by lysosomes.
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Apoptosis Induced by Decreased Ubiquitination Is Not Due to an Accumulation of the Pro-apoptotic Bax Protein-- Having established that Bcl-2 does not prevent the accumulation of short-lived/aberrant proteins at the nonpermissive temperature, we wanted to know whether expression of its pro-apoptotic partner Bax was enhanced as well. There is emerging evidence that increased Bax expression sensitizes cells for apoptosis (44). In addition, as compared with Bcl-2, which is an extremely stable protein, Bax has been shown to exhibit a high turnover (45). Whether this is due to a degradation by the ubiquitin/proteasome system has not yet been established. We performed an anti-Bax Western blot of H38-5, ts20pMV12, ts20Bcl-2#7, and ts20Bcl-2#13 extracts obtained from cells either grown at 34 °C or 39 °C. Fig. 5E shows that both H38-5 and ts20 cells express endogenous 21-kDa Bax at 34 °C. However, this level did not increase in either cell derivative at the nonpermissive temperature. This indicates that Bax is not degraded by the ubiquitin/proteasome system (Fig. 5E) and that apoptosis of ts20 cells at 39 °C is not due to an accumulation of the Bax protein.
Bcl-2 Bypasses G1/S and G2/M Cell Cycle Checkpoints-- Our finding that ts20Bcl-2#7 cells still proliferated (Fig. 1B) in the presence of high levels of p53, p27kip1, and cyclin B1 (Fig. 5, C, D, and F) suggested that Bcl-2 may have been able to disrupt G1/S or G2/M cell cycle checkpoints. To provide further evidence for this notion, we determined the cell cycle profile of ts20pMV12 and ts20Bcl-2#7 cells at both 34 °C and 39 °C. We used a flow cytometry method on DAPI-stained DNA (31) that allowed the simultaneous analysis of apoptotic cells with fragmented DNA (sub-G1 peak fraction) and nonapoptotic cells in various cell cycle states (DNA ploidy). At 34 °C, both ts20pMV12 and ts20Bcl-2#7 exhibited cell cycle profiles typical of adherent, asynchronously growing fibroblasts, i.e. 60% in G1, 23% in S, and 17% in G2/M (Fig. 6). As shown previously (29), ts20pMV12 cells were selectively lost from the S phase and majorly accumulated in the G1 and G2/M phase at 36 h after shift to 39 °C (Fig. 6). In addition, up to 50% of the cells displayed a DNA stainability lower than that of G1 cells, indicating that they contained low molecular weight DNA characteristic of apoptotic cells. Interestingly, none of these changes were seen with ts20Bcl-2#7 cells (Fig. 6). A significant proportion of these cells (20-25%) were still found in the S phase at 39 °C, indicating that they were cycling and thus bypassing the cell cycle checkpoints usually imposed by accumulating levels of p53, p27kip1, or cyclin B1.
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Cycloheximide Prevents Accumulation of Short-lived proteins at 39 °C and Blocks Apoptosis Induced by Decreased Ubiquitination-- The data so far are consistent with the idea that one or several short-lived proteins that accumulate in response to the inactivation of the E1 enzyme cause apoptosis. To support this hypothesis, we pretreated ts20pMV12 cells with cycloheximide for 2 h before exposing them to 39 °C. Because short-lived proteins are rapidly lost in cycloheximide-treated cells, they would not be available to trigger apoptosis at 39 °C. We found that this is indeed the case. ts20pMV12 cells pretreated with 5 µg/ml cycloheximide and then grown at 39 °C in the presence of lower concentrations of cycloheximide (1 µg/ml), did not accumulate short-lived proteins such as p53 (Fig. 7B) and did not die by apoptosis, as judged by morphological changes (Fig. 2A), chromatin condensation (Fig. 2C), and DNA fragmentation (Fig. 2B). Cycloheximide even had an effect when given after apoptosis induction at 39 °C. In this case, depending on the time of cycloheximide addition, p53 (Fig. 7D) rose to detectable levels, but apoptosis was still delayed (data not shown). Because less protein was synthesized in cycloheximide-treated cells, it was less ubiquitinated than in untreated cells at both 34 °C and 39 °C (Fig. 7, A and C). These results indicate that high levels of short-lived proteins are required to promote apoptosis. If their expression is diminished due to cycloheximide treatment, their apoptotic activity is reduced markedly.
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Bcl-2 Protects against Apoptosis Induced by the Simultaneous Inhibition of Ubiquitination and Proteasomal Degradation-- How does Bcl-2 neutralize the action of apoptotic short-lived proteins? It may direct these proteins to the proteasomal or lysosomal degradation system without prior ubiquitination, although such a mechanism does not seem to account for p53, p27kip1, and the cyclins D1 and B1 (see Fig. 5, C, D, and F). We tested this hypothesis by blocking proteasomal or lysosomal activity using respective inhibitors. Treatment of ts20pMV12 cells with 5 µM amounts of the proteasome inhibitor lactacystin (28) markedly accelerated apoptosis at 39 °C (Fig. 8A). This can be explained by a further accumulation of short-lived proteins, which, in the absence of lactacystin, were still partially ubiquitinated and degraded due to an inefficient inactivation of the E1 enzyme at 39 °C. Importantly, ts20Bcl-2#7 and, to a lesser extent, ts20Bcl-2#13 cells were still protected from apoptosis induced by a lactacystin treatment at 39 °C (Fig. 8A). Similarly, MG132, a effective inhibitor of the proteasome, calpains, and some lysosomal proteases (cathepsin B) (46) triggered rapid and almost complete loss of ts20pMV12 cells at 39 °C, whereas ts20Bcl-2 cells were effectively spared (Fig. 8B). This cell loss was by apoptosis as the genomic DNA of MG132-treated ts20pMV12 cells was fragmented (data not shown). These results indicate that Bcl-2 does not bypass ubiquitination to directly stimulate the degradation of short-lived, apoptosis-promoting proteins by the proteasome or lysosomal systems. It may instead inhibit the apoptotic activity of these proteins by direct protein-protein interactions.
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DISCUSSION |
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The present study describes a novel, caspase-independent form of apoptosis that can be effectively delayed by Bcl-2 overexpression. The molecular players in this process appear to be short-lived or aberrant proteins that are usually degraded by the ubiquitin/proteasome system. Due to diminished ubiquitination, these proteins accumulate to levels that are probably sufficient to trigger apoptosis without the participation of caspases. Because Bcl-2 overexpression still allows these proteins to accumulate but not to trigger apoptosis, they represent attractive targets for the death-protective action of Bcl-2.
Ubiquitin-dependent degradation mainly serves to rid the cell of abnormal and misfolded proteins and to limit the time and amounts of availability of critical regulatory proteins (short-lived proteins) (27). This is essential for numerous cellular processes such as cell cycle control (38), gene transcription (47), chromatin maintenance (48), cytoskeletal (49) and surface receptor functions (50), intracellular trafficking (51), antigen presentation (52), and stress responses (53). It is therefore conceivable that defects in the ubiquitination pathway perturb cell homeostasis and, if becoming extensive, can promote cell death. The present data support this notion and confirm previous reports showing that proteasomal inhibition induced apoptosis (54, 55) and an up-regulation of ubiquitination prevented cell damage generated by heat shock or hypoxia (ischemia) (53, 56).
Nevertheless, pro-apoptotic activities of the ubiquitin system have also been reported. Muscle cell death in the hawkmoth Manducta sexta involved the transient up-regulation of ubiquitin mRNA (57, 58). This, however, occurred at a late stage of the process and probably served to eliminate cytoplasm before phagocytosis rather than to execute cell death. On the other hand, certain forms of apoptosis in neurons and thymocytes were shown to depend on a functional proteasome (59, 60), presumably because a recently identified proteasomal caspase was required to activate death effectors in an early phase of apoptosis (61). However, neurons and thymocytes do not differ from other cells in being susceptible to apoptosis when persistently exposed to proteasome inhibitors (59, 60).
Short-lived Proteins as Death Effectors or Inhibitors of Survival
Factors--
What might be the effector molecules that trigger
apoptosis in response to decreased ubiquitination? Whereas aberrant
proteins may interfere with vital cellular functions in rather
nonspecific ways, accumulated short-lived proteins may have selective
targets. Two mechanisms of action can be envisaged. First, short-lived proteins may be death effectors at high levels. They would be present
in minute, nontoxic amounts in normal cells but accumulate to levels
that activate some unknown "death substrates" in cells with a
defective E1 enzyme. If apoptosis is induced by other stimuli such as
staurosporine, tumor necrosis factor , or Fas/APO, the short-lived
proteins may accumulate due to a posttranslational modification that
prevents their ubiquitin-dependent degradation. Various
transcription factors such as p53, Myc, Jun, E2F, and cell cycle
components such as the cyclins and cdc25 are rapidly degraded by the
ubiquitin degradation pathway following phosphorylation, dephosphorylation, or other modifications (41, 62-66). Interestingly, most of these proteins promote apoptosis when accumulating or forced to
be overexpressed (43, 67-71). It is, however, unknown how their usual
function as cell cycle or transcriptional regulators is converted into
an apoptotic activity. According to the data here, this may be done by
changing their half-lives. A similar mechanism has already been
suggested for the conversion of these proteins into oncogene products
(65) and indicates that the ubiquitin system may be an attractive,
common regulator of tumorigenesis and apoptosis.
Short-lived Proteins as Targets of Bcl-2-- In this study, Bcl-2 acts after the accumulation of aberrant or short-lived proteins to produce two cellular effects: protection against apoptosis and disruption of G1/S and G2/M cell cycle checkpoints, usually imposed by persistently high levels of p53, p27kip1, or cyclin B1, respectively. We do not yet know whether these two effects are causally linked nor how Bcl-2 achieves to regulate them. Three mechanisms can be envisaged.
(i) Bcl-2 directs accumulating proteins to a ubiquitin-independent proteolytic degradation system. Such "antizymes" have been identified for p53 (E6) (47), cyclin A/B2 (p34cdc2) (74), and ODC (ODC antizyme) (75) and serve to accelerate the degradation of the respective short-lived protein. The death substrates that may be "antizymed" by Bcl-2 are, however, distinct from p53, p27kip1, or the cyclins D1 and B1 because the latter accumulate to similar levels in vector control and Bcl-2-overexpressing cells. Moreover, the degradation machinery of these substrates are neither the proteasome nor lysosomes. Proteasomal inhibitors as well as lysosomotropic agents enhance the accumulation of short-lived proteins and provoke apoptosis much faster than decreased ubiquitination alone, but Bcl-2 is still effectively death-protective. These data do not, however, exclude the possibility that Bcl-2 exploits another proteolytic system to degrade specific short-lived death effectors. Moreover, Bcl-2 may sequester short-lived proteins into lysosomes where they are not degraded but unable to trigger apoptosis. (ii) Alternatively, Bcl-2 converts aberrant or short-lived proteins into an apoptosis-incompetent conformation. This could be best explained by a chaperoning function of Bcl-2. Chaperones like the well known heat shock proteins are ATPases that hold aberrant, denatured proteins in a nontoxic conformation. Bcl-2 cooperates with heat shock proteins to protect cells from lethal doses of heat shock (76), and certain functions of Bcl-2 such as its antioxidant activity can also be performed by heat shock proteins (77). However, Bcl-2 lacks an ATP binding site and may require other chaperones to effectively neutralize aberrant proteins (co-chaperone model). Such an activity of Bcl-2 would explain why this protein is "sticky" and interacts with numerous substrates such as R-Ras, Raf, the prion protein, Nips, and others (78), which cannot be easily defined as physiological partners of Bcl-2 (79). Apart from simply neutralizing aberrant protein, Bcl-2 may interact with specific short-lived death effector proteins and inhibit their apoptotic action. Two attractive targets are p53 and Bax. It has been reported that overexpressed p53 can induce apoptosis (70). Thus, the death described herein may be p53-dependent. Preliminary data, however, indicate that conditional overexpression of p53 in ts20 cells does not lead to apoptosis but to growth arrest (data not shown). In addition, the expression of Bax, a p53-inducible gene (80), does not increase upon p53 accumulation in ts20 cells at 39 °C. Thus, neither Bax nor p53 seem to be the targets of Bcl-2 and mediators of the apoptotic response induced by decreased ubiquitination. (iii) Finally, Bcl-2 could replace a survival factor whose activity is blocked by decreased ubiquitination. As discussed above, the activity of the survival factor c-Rel/NFCycloheximide Effects Support the Involvement of Short-lived Proteins in Apoptosis-- It has become widely accepted that the apoptotic effector machinery is constitutively expressed in mammalian cells (26). If all death effectors were stable proteins, a blockage of their synthesis by cycloheximide would not immediately affect their apoptotic potential. However, if some of them were short-lived proteins, they would be rapidly eliminated from the cell. This is what seems to happen with apoptosis induced by decreased ubiquitination or the additional treatment with proteasome inhibitors. A short-term cycloheximide treatment before apoptosis induction entirely abrogates the apoptotic response. Even when given shortly after apoptosis induction, cycloheximide still had a slight protective effect, presumably because aberrant or accumulated short-lived proteins failed to reach the apoptotic threshold level. These results are in agreement with previous data in neurons, where cycloheximide saved the cells from apoptosis due to nerve growth factor withdrawal up to a point of no return (approximately 18 h) (81). Based on the latter finding, it was proposed that the apoptosis-promoting factors were de novo synthesized death effector proteins. Here, we suggest an alternative view to this paradigm: the accumulation of pre-existing death effector proteins.
Caspase-independent Apoptosis Blockable by Bcl-2: Existence of Novel Death Effectors?-- To our surprise, apoptosis induced by decreased ubiquitination was caspase-independent. This was because (i) the death effector proteases caspase-3 and -6 were not activated; (ii) PARP, a specific substrate of both effector caspases-3 and -7 and lamin A, a specific substrate of caspase-6, were not cleaved; and (iii) both Z-DEVD-fmk, a caspase-3/7-specific inhibitor, and Z-VAD-fmk, a general caspase inhibitor, were incapable of blocking this type of apoptosis. It is difficult to imagine that caspases exist that would not be blocked by any of these inhibitors. However, we still cannot exclude such a possibility. In this respect, it would have been informative to test another, well known general caspase inhibitor, the baculovirus p35 protein (82), for its effect on this type of apoptosis; unfortunately, we did not succeed in achieving high level expression of this protein in ts20 cells. Irrespective of the possible involvement of unknown caspases, the apoptosis described herein was effectively blocked by Bcl-2. This shows for the first time that Bcl-2 targets molecules in addition to the presently known caspases to achieve death protection.
Recent reports have provided evidence for caspase-independent steps of apoptosis. (i) The overexpression of Bax activated caspase-3 but the resulting apoptosis was not blocked by Z-VAD-fmk (83). (ii) Degranulation-initiated target cell lysis by cytotoxic T cells could not be blocked by caspase inhibitors, yet DNA fragmentation could (84, 85). (iii) Nuclear fragmentation induced by DNA damage or by the overexpression of myc or Bak were blocked by Z-VAD-fmk, but other apoptotic features such as membrane blebbing persisted for days and the cells could not be rescued from the death fate (86). (iv) Bak and the C. elegans death gene product ced-4 induced cell death in yeast with morphological changes similar to those with apoptosis in mammalian cells, but no caspases have yet been described in yeast (87, 88). These data indicate that caspases do not control all aspects of apoptosis. What might be the mechanisms of caspase-independent apoptosis? Based on the finding that Z-VAD-fmk did not prevent the rapid fall in the mitochondrial membrane potential in response to Bax overexpression (83), it was proposed that caspase-unrelated death effectors may perturb mitochondrial functions leading to an opening of the permeability transition (PT) pore or the release of pro-apoptotic factors such as cytochrome c and an apoptogenic protease (2). Indeed, mitochondrial perturbations are associated with many forms of apoptosis (2), and Bcl-2 can block the fall in the mitochondrial membrane potential as well as the appearance of cytochrome c and the apoptogenic protease in the cytoplasm (2, 89, 90). However, the treatment of ts20 cells with the PT pore blockers bongkrekic acid and cyclosporine A failed to delay apoptosis induced by decreased ubiquitination (data not shown), thus excluding the involvement of the PT pore in the caspase-independent steps of this type of apoptosis. Another attractive, caspase-independent mediator of apoptosis is the nematodal ced-4 protein. Based on genetic analysis in C. elegans, the ced-4 protein participates in caspase (ced-3)-dependent and -independent forms of apoptosis that can both be blocked by ced-9/Bcl-2 (13). In addition, ced-4-induced (and, so far, ced-3/caspase-independent) chromatin condensation and killing in yeast is inhibited by direct physical association with ced-9 (88). The recent isolation of a mammalian homologue of ced-4, called Apaf-1 (25), will make it possible to test whether ced-4-like molecules are short-lived targets of Bcl-2 for the inhibition of both, caspase-dependent and -independent forms of apoptosis. ![]() |
ACKNOWLEDGEMENTS |
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We are grateful to D. W. Nicholson for the anti-caspase 3 antibody p17, to G. G. Poirier for the anti-PARP, to R. Iggo for the anti-p53 antibody Ab240, to Le Sun for the anti-Bax antibody, and to Proscript Inc. for MG132. We thank A. Conzelmann, S. Rusconi, J.-L. Dreyer, and C. Waeber for critically reading the manuscript.
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FOOTNOTES |
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* This work was supported by Grants 31-36152.92 and 31-34600.92 from the Swiss National Science Foundation, Grant 421 from the Swiss Cancer League, and a grant from the Foundation for Aging Research (all to C. B.); by Grant AG04821 from the NIA, National Institutes of Health (to H. L. O.); and by Grant GM34009 from the National Institutes of Health (to A. L. H.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
** To whom correspondence should be addressed. Tel.: 41-26-300-86-52; Fax: 41-26-300-97-35; E-mail: christoph.borner{at}unifr.ch.
1 The abbreviations used are: Z-DEVD-fmk, N-benzyloxycarbonyl-Asp-Glu-Val-Asp-fluoromethylketone; CHX, cycloheximide; DAPI, 4',6'-diamidino-2-phenylindole; MG132, carbobenzoxyl-leucinyl-leucinyl-leucinal-H; PARP, poly(ADP-ribose) polymerase; PT, permeability transition; PVDF, polyvinylidene difluoride; Ub, ubiquitin; Z-VAD-fmk, N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone; PAGE, polyacrylamide gel electrophoresis; Pipes, 1,4-piperazinediethanesulfonic acid; E1, ubiquitin-activating enzyme; E2, ubiquitin carrier protein; E3, ubiquitin-protein isopeptide ligase.
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