From the Fred Hutchinson Cancer Research Center,
A2-025, Seattle, Washington 98109 and the § Division of
Neuroscience, Children's Hospital, and Department of Neurobiology,
Harvard Medical School, Boston, Massachusetts 02115
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ABSTRACT |
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Reactive oxygen species (ROS) have been
implicated in the induction of apoptosis by tumor necrosis factor-
(TNF
) and other cytotoxic insults, although the molecule(s)
regulated by ROS in TNF
signaling have not been identified.
Apoptosis signal-regulating kinase 1 (ASK1) is a member of the
mitogen-activated protein kinase kinase kinase (MAPKKK) superfamily
that has been shown to be activated during TNF
-induced apoptosis.
ASK1 increases apoptosis when overexpressed, but the mechanism of ASK1
activation and the mechanisms of ASK1-induced apoptosis are unclear. We
now report that hydrogen peroxide induces the activation of ASK1 in 293 cells. TNF
-induced activation of ASK1 was inhibited by antioxidants.
Hydrogen peroxide-induced apoptosis was markedly enhanced by the
expression of ASK1. These results suggest that TNF
-induced
activation of ASK1 is mediated by ROS. We also examined how ASK1
activity is regulated by ROS. We found that ASK1 formed dimers or
higher order oligomers in 293 cells. TNF
or hydrogen peroxide
treatment increased the dimeric form of ASK1, and pretreatment with
N-acetylcysteine decreased it. Furthermore, synthetic
dimerization of an ASK1-gyrase B fusion protein by coumermycin resulted
in substantial activation of ASK1, suggesting that dimerization of ASK1
is sufficient for its activation. These results taken together suggest
that TNF
causes ASK1 activation via ROS-mediated dimerization of
ASK1.
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INTRODUCTION |
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The mitogen-activated protein kinases (MAPKs)1 participate in intracellular signaling pathways that are activated by extracellular stimuli in a variety of cell types (1-3). In mammalian cells, there are at least three distinct members of the MAPK family: MAPK (also known as extracellular signal-regulated kinase), stress-activated protein kinase (SAPK) (also known as c-Jun NH2-terminal kinase (JNK)), and p38. The "classical" MAPK is known to be activated and involved in growth factor-mediated cell proliferation and differentiation (1, 3). In contrast, SAPK and p38 are activated in response to proinflammatory cytokines and environmental stresses such as UV and osmotic shock and are implicated in inflammatory responses, cell cycle arrest, DNA repair, and apoptosis (3-5).
Considerable progress has been made in identifying the upstream kinases that regulate each member of the MAPK family. The central core of each MAPK pathway is a conserved cascade of three protein kinases, which are commonly referred to as MAPK, MAPK kinase (MAPKK), and MAPKK kinase (MAPKKK) (1, 2). The MAPKKs for the "classical" MAPK are MKK1/MEK1 and MKK2/MEK2; those for SAPK are SEK1/MKK4 and MKK7; and those for p38 are MKK3 and MKK6. At the MAPKKK level, Raf-1, B-Raf, and c-Mos (and MEKK1 and Tpl2) are known to activate the classical MAPK pathway, whereas MEKK1, MEKK2, MEKK3, MEKK4/MTK1, Tpl2/Cot, MLK3/PTK1/SPRK, MUK, TAK1, and ASK1/MAPKKK5 have been reported to activate the SAPK and p38 pathways (3, 6). MAPKs are activated by dual phosphorylation on Thr and Tyr residues catalyzed by MAPKKs, which in turn are activated by dual phosphorylation on Ser and Ser/Thr residues catalyzed by MAPKKKs. The activation of the MAPKKKs in the classical MAPK pathway has been studied extensively. The activation of Raf-1 has been shown to involve Ras-mediated membrane recruitment, phosphorylation at critical sites (7), and possibly dimerization (8, 9). However, the activation mechanism of MAPKKKs in the "stress-activated" MAPK pathways remains largely unknown and is one of the critical unanswered questions in signal transduction.
ASK1 (also called MAPKKK5) is a member of the MAPKKK family, which can
activate MKK4 and MKK7 (SAPK pathway) and MKK3 and MKK6 (p38 pathway)
by direct phosphorylation (10, 11). Several lines of evidence suggest
that ASK1 is involved in TNF-induced apoptosis signaling. First,
ASK1 is activated by TNF
treatment in many cells; second, the
overexpression of ASK1 induces apoptosis in stably transfected Mv1Lu
cells; and third, the expression of a dominant-negative ASK1 inhibits
TNF
-induced apoptosis in Jurkat cells (11). However, it remains to
be determined how ASK1 is regulated by TNF
signaling.
TNF is a multifunctional proinflammatory cytokine, which induces
apoptosis or other responses depending on the cellular context. The
effects of TNF
are mediated by cell surface receptors (p55 TNF-R1
and p75 TNF-R2), their adaptor proteins, and intracellular messengers
(12, 13). Among them, ROS have been proposed to act as signaling
intermediates for TNF
-induced apoptosis, because they are produced
in response to TNF
stimulation (14-17) and because antioxidants
inhibit TNF
-induced cell death (18-28). Furthermore, sensitivity to
TNF
is inversely correlated with the levels of superoxide dismutase
(29, 30) and glutathione (28, 31), the most abundant antioxidants in
cells. These studies prompted us to examine whether ASK1 is regulated
by ROS in TNF
signaling. We have found that ROS can activate ASK1.
We further examined the molecular mechanism of ASK1 activation induced
by ROS and found that dimeric forms of ASK1 exist in ROS-stimulated
cells and that in vivo dimerization of ASK1 is sufficient
for its activation. Thus, we provide the first evidence that
dimerization is important for activating an enzyme that regulates the
stress-activated MAPK pathways.
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EXPERIMENTAL PROCEDURES |
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Cell Culture and Reagents--
Human embryonic kidney 293 cells
were maintained in Dulbecco's modified Eagle's medium containing 10%
fetal bovine serum. For hydrogen peroxide treatment, media were
replaced with Opti-MEM (Life Technologies, Inc.) without serum. Mink
lung epithelial (Mv1Lu) cells were stably transfected with either
vector alone (pMEP4) or wild-type ASK1-pMEP4 as described previously
(11). For induction of ASK1, the stable transformant cells were
incubated in Dulbecco's modified Eagle's medium containing 0.1%
serum in the presence of 50 µM ZnCl2
overnight. Recombinant human TNF was from Collaborative Biomedical
Products. Hydrogen peroxide, coumermycin, novobiocin,
N-acetylcysteine (NAC), catalase, pyrrolidine dithiocarbamate, and protein G-Sepharose were from Sigma.
2',7'-dichlorodihydrofluorescein diacetate was from Molecular Probes,
Inc. NAC was used after adjusting the pH to 7 with NaOH.
1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) was
from Pierce. Anti-hemagglutinin (HA) antibody (12CA5) (32) was a kind
gift from Dr. Davis P. Creemer and also purchased from Boehringer
Mannheim. Anti-Myc antibody (9E10) (33) was prepared from hybridoma
cells.
Expression Vectors and Transfections--
pcDNA3-ASK1,
pME18-HA-MKK6, and pSR-HA1-SAPK were described previously (11).
pME18-HA-ASK1 was constructed by ligating a
SalI-NotI fragment containing ASK1 into
pME18-HA. pME18-Myc-ASK1 was constructed by ligating ASK1 cDNA into
the SalI site of pME18-Myc. For constructing this plasmid,
SalI sites were created at both ends of ASK1 by polymerase
chain reaction-amplifying it with the primers
5'-CCGCGGTCGACGACTGTGAAAGTGACTTGCT-3' and
5'-GGCCGTCGACCCAGTCTGTTTGTTTCGAAAGTC-3'. The modified gyrase B vector,
which has a translation initiation site, gyrase B, and a linker for
fusion protein with Bluescript KS backbone, was generously provided by
Drs. Michael A. Farrar and Roger M. Perlmutter (University of
Washington) (8). SpeI sites were added to both ends of ASK1
by polymerase chain reaction-amplifying the full-length ASK1 cDNA
with the primers 5'-GGGGACTAGTGCGGCCGCTCAAGTCTGTTTGTTTCGAAA-3' and
5'-GGGGACTAGTATGAGCACGGAGGCGGACGAGGGCATCAC-3'. The amplified fragment was inserted into the SpeI site of the modified
gyrase B vector. The gyrase B-linker-ASK1 fragment was cut out with
NotI and inserted into NotI site of pcDNA3
(Invitrogen). For control experiments, gyrase B without ASK1 was also
introduced into pcDNA3. For transfections, 7 × 105 cells in 60-mm dishes were transfected with 1-5 µg
of DNA in total by using lipofectamine (Life Technologies, Inc.)
according to the manufacturer's instructions.
Kinase Assays--
Cells (60-mm dish) were extracted with a
buffer solution containing 20 mM Tris-Cl (pH 7.5), 10 mM -glycerophosphate, 5 mM EGTA, 5 mM NaF, 1 mM NaPPi, 150 mM NaCl,
1% Nonidet P-40, 2 mM dithiothreitol, 1 mM
sodium vanadate, 0.5% (v/v) aprotinin, and 1 mM
phenylmethylsulfonyl fluoride. After clarification by centrifugation, HA-ASK1, HA-SAPK, HA-MKK6, or Myc-ASK1 were precipitated with anti-HA
or anti-Myc with protein G-Sepharose. After washing the immunoprecipitates three times with the extraction buffer and once with
a buffer solution containing 20 mM Tris-Cl (pH 7.5), 10 mM MgCl2, 2 mM EGTA, 1 mM dithiothreitol, and 1 mM
phenylmethylsulfonyl fluoride, they were subjected to kinase assays.
For the ASK1 assay, the immunoprecipitates were incubated with 0.2 µg
of His-tagged MKK6 before adding 2 µg of His-tagged kinase-inactive
p38 as described previously (11). His-tagged kinase-inactive p38 and
GST-c-Jun were used as substrates for MKK6 and SAPK, respectively
(11).
Chemical Cross-linking-- Cells (60-mm dish) were washed with phosphate-buffered saline and homogenized with 150 µl of phosphate-buffered saline containing 1 mM dithiothreitol and 1 mM phenylmethylsulfonyl fluoride. After centrifugation at 10,000 × g for 3 min, 50 µl of the supernatants were incubated with 10 mM EDC for 10 min at 30 °C. To stop the reaction, EDC was removed by spin column (ProbeQuant G-50 MicroColumns; Amersham Pharmacia Biotech), and the eluates were boiled with Laemmli sample buffer.
Apoptosis Assay-- The terminal deoxynucleotidyltransferase end labeling assay was carried out using the In Situ Cell Death Detection Kit (Boehringer Mannheim) according to the manufacturer's instructions.
ROS Assay--
Intracellular ROS production was measured by the
method of Bass et al. (34) as modified by Bae et
al. (35). Briefly, dishes treated with or without TNF for 10 min were washed with Earle's balanced salt solution without phenol red
and incubated in the dark for 5 min in the presence of 5 µM dichlorodihydrofluorescein diacetate. When
dichlorodihydrofluorescein diacetate is oxidized within the cell, it
becomes a highly fluorescent 2'7'-dichlorofluorescein and can be
detected (excitation, 488 nm; emission, 515-540 nm).
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RESULTS |
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To study the activation mechanism of ASK1 in response to TNF
stimulation, we first examined whether ceramide can activate ASK1.
Ceramide is known to be generated in response to TNF
and can induce
apoptosis in U937, lymphoblastoid, and endothelial cells (36-38).
Human embryonic kidney 293 cells were transfected with HA-tagged ASK1
and treated with a membrane-permeable ceramide analogue,
C2-ceramide. HA-ASK1 was immunoprecipitated, and its activity was determined by a coupled kinase assay using recombinant MKK6 and a kinase-negative p38. As shown in Fig.
1A, the
C2-ceramide treatment had little effect on ASK1 activity
(~1.3-fold activation). However, the same concentration of
C2-ceramide induced apoptosis in 293 cells (data not
shown), suggesting that ceramide may not be a major mediator of ASK1
activation.
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TNF treatment increases ROS, such as H2O2,
that are thought to be essential for TNF
-induced apoptosis (see
Introduction). In fact, the ROS level measured by
dichlorodihydrofluorescein diacetate was increased after 15 min of
TNF
treatment in Mv1Lu cells (5.8-fold on average) in which ASK1
activation is highest at 30 min after TNF
treatment (11). Therefore,
it is possible that ROS is involved in ASK1 activation.
H2O2 treatment induced apoptosis in a
dose-dependent manner (EC50 ~ 50 µM) as determined by the appearance of apoptotic bodies
and nuclear condensation in 293 cells (data not shown). Importantly,
H2O2 treatment significantly increased ASK1
activity in 293 cells transiently transfected with HA-ASK1 (Fig.
1A). Activation of ASK1 by H2O2 was
detected not only by a coupled kinase assay but also by an
autophosphorylation assay for ASK1 (Fig. 1B). The doses of
H2O2 required for the activation of ASK1
(EC50 ~30 µM) were approximately the same
as those required for inducing apoptosis (Fig. 1B). The
activation of ASK1 by H2O2 treatment was
sustained for at least 2 h (Fig. 1C). Endogenous ASK1
was also activated by H2O2 treatment as
determined by immunoprecipitation with an anti-ASK1 antibody (Fig.
1D). In contrast, treatment with 10 µg/ml anisomycin did
not increase ASK1 activity (Fig. 1A), although anisomycin is
a strong activator of the SAPK and p38 pathways. This implies that ASK1
is only activated by a subset of the stimuli that activate p38 and
SAPK. In fact, it is not clear that ASK1 is a major MAPKKK for p38 and
SAPK in TNF signaling.
Activation of ASK1 by either TNF or H2O2 was
3-5-fold compared with basal level (Fig. 1). The -fold activation of
MAPKKKs is generally low compared with that of MAPKs. A model proposed by Ferrell might explain why a small change in activity of a MAPKKK can
trigger robust activation of a MAPK; the requirement for dual phosphorylation to activate the MAPKK and MAPK, the three-kinase cascade (MAPKKK-MAPKK-MAPK), and near saturation of the enzymes produce
multistep cooperativity or "ultrasensitivity" and thus create a
switch-like response from a graded stimulus (39).
Since H2O2 activated ASK1 in the cell, we next
examined if ROS are required for TNF-induced activation of ASK1. We
pretreated 293 cells with an antioxidant, NAC, and then treated the
cells with TNF
. In the presence of 20 mM NAC, the
activation of ASK1 by TNF
treatment was markedly inhibited, as shown
in Fig. 2. The basal activity of ASK1 was
also reduced by NAC treatment (~30% reduction) (Fig. 2). In addition
to NAC, catalase (3000 units/ml) (a peroxide-scavenging enzyme) and
pyrrolidine dithiocarbamate (100 µM) reduced the
TNF
-induced activation of ASK1 (data not shown; Fig. 2). These
results suggest that the production of ROS is essential for ASK1
activation in TNF
signaling.
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Since ASK1 was activated by H2O2 and ASK1 has been implicated in apoptosis signaling, we tested whether ASK1 expression enhances H2O2-induced apoptosis. ASK1 was inducibly expressed in Mv1Lu cells stably transfected with an ASK1 construct driven by the metallothionein promoter (11). Only a small fraction of cells grown in serum undergo apoptosis by ASK1 expression (Fig. 3).2 Treatment of the ASK1-expressing cells with 50 µM H2O2 for 3 h effectively induced apoptosis (~90% of the cells), whereas less than 10% of the cells underwent apoptosis in the Mv1Lu cells transfected with an empty vector (Fig. 3). More than 200 µM H2O2 was required to induce apoptosis in the latter cells. Therefore, ASK1 synergized with H2O2 in inducing apoptosis, which is consistent with the hypothesis that ASK1 plays a role in ROS-mediated apoptosis signaling. A kinase-dead mutant (K709R) ASK1 did not inhibit H2O2-induced apoptosis in 293 cells (data not shown), suggesting that ASK1 is not the only target for H2O2 in inducing apoptosis in 293 cells.
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We then investigated the molecular mechanism of ASK1 activation induced by ROS. Because Raf1, another member of the MAPKKK superfamily, has recently been reported to be activated by dimerization, we examined whether ASK1 also forms a dimer. First we carried out chemical cross-linking experiments using EDC, which cross-links between carboxyl groups and amine groups. After treatment with or without EDC, the cell lysates were subjected to immunoblotting with anti-HA to determine the mobility of HA-ASK1. In addition to the monomeric HA-ASK1 (~190 kDa), some bands at the position around 350 kDa were detected only in the lysate incubated with EDC (Fig. 4). The mobility of this band appeared to correspond to the molecular weight of a dimer of ASK1. To confirm that ASK1 forms a dimer, Myc-tagged ASK1 and HA-tagged ASK1 were either co-expressed or expressed separately in 293 cells to see if they would coprecipitate. Myc-tagged ASK1 was detected in anti-HA immunoprecipitates from the cells expressing both HA- and Myc-tagged ASK1 but not from the cells expressing only either Myc- or HA-ASK1 (Fig. 5). In the converse experiment, HA-tagged ASK1 was detected in anti-Myc immunoprecipitates only from the cells expressing both HA- and Myc-ASK1 (Fig. 5). These results suggest that ASK1 either forms dimers or higher order oligomers.
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Importantly, both TNF and H2O2 treatment
moderately increased the amount of ASK1 at the dimer position (Fig. 4).
Moreover, pretreatment with NAC markedly reduced the amount of ASK1 at
the dimer position (Fig. 4). Therefore, TNF
and its downstream
mediator H2O2 may promote dimerization of ASK1
within the cell.
The next important question is whether ASK1 can be activated by dimerization. To address this, we produced a fusion protein between gyrase B and ASK1 to determine if dimerization of ASK1 is sufficient for its activation. The application of coumermycin to cells induces the dimerization of gyrase B, but novobiocin, a monovalent analogue of coumermycin, does not (Ref. 8; Fig. 6A). Activation of ASK1 within the cell was monitored by the activity of a co-transfected substrate, MKK6. Treatment of the gyrase B-ASK1-transfected 293 cells with coumermycin induced activation of ASK1 efficiently (Fig. 6B). In contrast, coumermycin had little effect on MKK6 activity in cells transfected with gyrase B without ASK1 (Fig. 6B). In addition, novobiocin did not induce gyrase B-ASK1 activation (Fig. 6B). The activation of gyrase B-ASK1 by coumermycin was also detected when the activity of ASK1 was monitored by the activity of another co-transfected substrate, SAPK (JNK) (Fig. 6C). These results strongly suggest that dimerization of ASK1 is sufficient for its activation.
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DISCUSSION |
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Very little is known about the activation mechanism of the MAPKKKs
in the stress-activated MAPK pathways, although the activation and the
functions of those pathways have been studied extensively (4). In this
report, we have investigated the activation mechanism of ASK1, a MAPKKK
that is involved in TNF-induced apoptosis. Since both ROS and ASK1
are thought to be important for propagating cytotoxic effects of
TNF
, we examined the relationship between ROS and ASK1. We propose
that ROS, especially H2O2, are involved in
TNF
-induced ASK1 activation based on the following evidence: 1)
TNF
induces both H2O2 production and ASK1
activation (11, 16, 17), 2) H2O2 treatment
increases ASK1 activity (this study), 3) antioxidant treatment
antagonizes TNF
-induced ASK1 activation (this study), and 4)
H2O2 treatment and ASK1 expression synergize in
inducing apoptosis (this study). These results are consistent with the
idea that ASK1 is activated downstream of H2O2
in TNF
signaling. Therefore, ASK1 as well as other potential ROS
targets (direct or indirect; cf. NF-
B, AP-1, MAPK,
phospholipase A2, protein kinase C, SAPK, p38, Lck, and
protein-tyrosine phosphatases (40-48)) might participate in mediating
ROS-induced cellular effects.
We further examined the molecular mechanism of ASK1 activation. The
results presented here demonstrate that ASK1 dimerizes in
vivo. In addition, we have shown here that 1) treatment of the
cells with either H2O2 or TNF increased the
dimeric form of ASK1, 2) NAC treatment decreased the dimeric form, and
3) synthetic dimerization of ASK1 by a gyrase B-coumermycin system was
sufficient for ASK1 activation in the cells. These data strongly
suggest that dimerization of ASK1 plays a role in its activation. This hypothesis is consistent with the fact that overexpression of ASK1
leads to a high level of basal activity in the absence of stimulation
(11). Also, it is possible that immunoprecipitation artificially
increases ASK1 activity by aiding dimer formation. Therefore, it would
be good to develop a method to assay ASK1 without immunoprecipitation.
It still remains to be addressed whether mechanisms other than
dimerization are involved in ASK1 activation and whether the
dimerization of ASK1 is required for its activation.
It is not clear whether ASK1 is a direct or indirect target for ROS.
In vitro incubation of an ASK1 immunoprecipitate with H2O2 did not increase its
activity.3 However, the
altered oligomerization of ASK1 induced by H2O2 and TNF may be due to changes in sulfhydryl oxidation in ASK1. When
we carried out anti-HA immunoblotting of extracts prepared from
HA-ASK1-expressing cells under nonreducing conditions, we observed
extra bands that were not detected in extracts prepared under standard
reducing conditions.3 This might suggest that ASK1 is
directly modulated by ROS. Interestingly, ASK1 has a cysteine-rich
domain in its NH2 terminus, which might be a target for the
action of ROS. In future studies, it will be important to determine
whether this domain is involved in the dimerization and activation of
ASK1.
How is ASK1 activated by dimerization? The dimerization might result in a conformational change that either renders ASK1 active or allows ASK1 to be activated by upstream activator(s). Alternatively, the dimerization could result in intermolecular autophosphorylation that is involved in activation, as is the case for receptor-tyrosine kinases. However, it is not clear thus far whether phosphorylation is important for ASK1 activation.
Previous papers have shown that Raf1 is also activated by synthetic dimerization using either the gyrase B-coumermycin system or the FKBP-FK1012A system (8, 9). Raf1 is also known to be activated by membrane recruitment by active Ras and subsequent phosphorylation by unknown kinase(s) (7). It is not clear if membrane recruitment and phosphorylation are required for Raf1 dimerization and vice versa. In contrast to Raf1, ASK1 is mostly localized in the cytoplasm even after stimulation with H2O2,3 so membrane recruitment is unlikely to be a prerequisite for activation of ASK1.
It is possible that binding protein(s) and phosphorylation are also involved in the activation of MAPKKKs in the stress-activated MAPK pathways. STE20, a yeast Ser/Thr kinase, is genetically upstream of STE11, a yeast MAPKKK. There exist a number of mammalian STE20 homologues such as PAKs, MST1/2/3, HPK1, SOK1, GCK, NIK, LOK, and KHS (3). SOK1 is activated by H2O2 (49), so it would be interesting to see if SOK1 can phosphorylate ASK1. Binding of the STE20-like kinases to MAPKKKs has been shown in some cases such as HPK1-MEKK1, NIK-MEKK1, and HPK1-MLK3 (50-52), but there is no evidence that STE20-like kinases can directly activate MAPKKKs. It is plausible that some MAPKKKs are downstream of the STE20-like kinases, since dominant-negative mutants of MEKK1, MLK3, or TAK1 have been reported to inhibit HPK1- or GCK-induced SAPK activation (10, 50, 52). In addition, MAPKKKs may be activated without STE20-like kinases. Recent papers have reported direct binding of Rac1 and Cdc42 to MEKK1, MEKK4, and MLK3 (53, 54), and this event may regulate activity. A newly identified protein, TAB1, binds to and activates TAK1 (55), but the precise activation mechanisms have not been determined.
In the present study, we have provided the first evidence that a stress-activated MAPKKK can be activated by dimerization. In addition to ASK1, other MAPKKKs such as MEKK1, MLK3, and TAK1 are highly active without activation/mutation when they are overexpressed in mammalian cells (49, 50), suggesting that they may also be stimulated by oligomerization. Furthermore, it is known that a yeast MAPK cascade, STE20-STE11-STE7-FUS3/KSS1, is activated by dimerization of a tethering protein STE5 and its activator STE4 (see Ref. 56 and references therein). Therefore, it is an intriguing possibility that dimerization/multimerization is a common mechanism for activation of some of the MAPKKK class of kinases.
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ACKNOWLEDGEMENTS |
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We thank Drs. Michael A. Farrar and Roger M. Perlmutter (University of Washington) for providing the plasmid containing gyrase B. We thank Drs. Michael E. Greenberg (Children's Hospital, MA) and Eisuke Nishida (Kyoto University) for helpful discussions and valuable support. We are also grateful to Drs. Ricardo Dolmetch and Jon Kornhauser for critical reading of the manuscript, to Drs. Hidenori Ichijo and Masao Saitoh (Cancer Institute, Japan) for stimulating discussions, and to the members of the Cooper and Greenberg laboratories for discussions and encouragement.
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FOOTNOTES |
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* This work was supported in part by the Japan Society for the Promotion of Science, by the Uehara Memorial Foundation (to Y. G.), and by National Institutes of Health Grant CA54786 (to J. A. C.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
¶ To whom correspondence should be addressed: Children's Hospital, 300 Longwood Ave., Enders 260, Boston, MA 02115. Tel.: 617-355-6332; Fax: 617-738-1542.
1 The abbreviations used are: MAPK, mitogen-activated protein kinase; MAPKK, MAPK kinase; MAPKKK, MAPK kinase kinase; SAPK, stress-activated protein kinase; JNK, c-Jun NH2-terminal kinase; ROS, reactive oxygen species; TNF, tumor necrosis factor; ASK1, apoptosis signal-regulating kinase 1; NAC, N-acetyl-L-cysteine; EDC, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride; HA, hemagglutinin.
2 H. Ichijo, personal communication.
3 Y. Gotoh, unpublished observation.
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REFERENCES |
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