From the Department of Pharmacology and
§ Department of Biochemistry and Molecular Biology, Mayo
Foundation and Graduate School, Rochester, Minnesota 55905
![]() |
ABSTRACT |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
We have analyzed the properties of cAMP response element-binding protein (CREB) in solution with emphasis on dimerization and effects of phosphorylation. Using a purified CREB fusion protein, a novel dye-label technique, and sedimentation equilibrium analysis, we directly and conclusively demonstrate that, unlike Jun and Fos, CREB dimerization is DNA-dependent. CREB exists primarily as a monomer in solution and cooperatively assembles on DNA to form dimers. Sedimentation equilibrium analysis also indicates that dimerization is unaffected by cAMP-dependent protein kinase-phosphorylation or by the symmetry of the cAMP-responsive element binding site. Filter binding assays reveal that CREB binding is unaffected by phosphorylation regardless of the symmetry of the cAMP-responsive element binding site. Our results suggest that structurally similar members of the same bZIP superfamily may differ significantly in their regulation at the level of dimerization.
![]() |
INTRODUCTION |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
The cAMP response element-binding protein (CREB)1 regulates genes that control diverse biological functions such as memory (1), gluconeogenesis (2), opiate tolerance (3), spermatogenesis (4), and circadian rhythms (5). CREB regulates genes via binding to the cAMP-responsive element (CRE) (6, 7). Additionally, CREB activity is modulated by phosphorylation at one or more sites within a kinase-inducible domain (8-11). Like most members of the bZIP superfamily, CREB has a modular structure consisting of distinct and separable DNA binding, dimerization, and transactivation regions (12). The kinase-inducible domain, which harbors a cluster of consensus phosphorylation sites for various protein kinases, lies within the two glutamine-rich transactivation domains (Q1 and Q2) toward the amino terminus of CREB (12, 13). The DNA binding (basic) and dimerization (leucine zipper) domains are adjacent to each other and occupy the carboxyl terminus of the protein (12).
While many protein kinases are able to modify CREB within its kinase-inducible subdomain, the effects of phosphorylation are not thoroughly understood. Most information on the effects of CREB phosphorylation has been obtained using cAMP-dependent protein kinase (PKA), which modifies a single serine site at position 133 (8, 9). Phosphorylation at Ser133 is known to increase the association of a 265-kDa nuclear protein, CREB-binding protein (CBP), and the homologous P300 (14, 15). Phosphorylation by PKA does not appear to alter the global conformation of CREB (16). Rather, recent structural studies support a model by which phosphorylation promotes direct electrostatic interaction between CREB and CBP/P300 (17, 18). The binding of CBP cannot, however, account for all of the effects of CREB phosphorylation. For instance, CREB can also be modified by calmodulin kinase II at positions 133 and 142 (10). However, a CREB mutant that mimics the calmodulin kinase II-phosphorylated protein is unable to support transcriptional induction under these conditions although CBP is still able to associate with CREB (19). Phosphorylation at positions 133 and 142 may have effects other than abrogation of CBP binding. It is possible that CREB phosphorylation influences properties of CREB such as binding affinity or dimerization.
It is generally accepted that bZIP proteins form dimers on DNA based on many in vitro and in vivo studies of yeast GCN4 and mammalian CREB/ATF and Jun/Fos families of transcription factors (20-25). However, it is not clear whether CREB is always self-associated as a dimer or whether the dimerization is always necessary for DNA binding and transcriptional regulation. For example, the CREB monomer has been reported to be the "active" form (26). The DNA binding affinity of some ATF family members is known to increase via dimerization upon association with the human T-cell leukemia virus transactivator TAX protein, suggesting that CREB may not always exist as a dimer in solution (27, 28). There has also been considerable disagreement as to whether phosphorylation of CREB influences its binding affinity or dimerization. In vitro, no differences in DNA binding of purified CREB have been observed upon PKA phosphorylation in gel shift assays (29, 30). However, in the presence of cAMP, the DNA binding activity of CREB is known to increase, particularly at nonsymmetric CREs (31, 32).
Previously, the dimerization state of CREB has been inferred from use of truncated partners (33) and gel electrophoresis techniques (29, 30) in which the gel matrix has the potential to form or to disrupt DNA-protein complexes. However, solution kinetic and spectroscopic studies have suggested that CREB dimerization may be DNA-dependent (34). If this is the case, then binding isoforms that have assumed dimer binding to the CRE may be incorrect. If and under what conditions the full-length CREB transits between monomer and dimer remains uncertain. Further, how phosphorylation influences dimerization and binding by various kinases is largely unknown.
To clarify the effects of phosphorylation on the dimerization potential of CREB, we have undertaken a systematic study of dimerization, binding, and transcriptional effects supported by CREB. In our studies, we employ exclusively solution techniques to avoid any potential artifacts produced by gel electrophoresis of CREB-DNA complexes. In this report, we focus on dimerization and binding properties of CREB before and after phosphorylation by PKA. The effects of PKA on the structure and function of CREB are well established, so there is no confusion concerning the site or extent of modification. In our experiments, we directly measure the dimerization state under solution conditions using sedimentation equilibrium analysis (35) including a novel dye-label technique and a purified CREB fusion protein. We demonstrate that, unlike Jun and Fos, CREB dimerization is completely DNA-dependent. CREB exists primarily as a monomer in solution and cooperatively assembles on DNA to form dimers. CREB binding as well as dimerization is unaffected by PKA-induced phosphorylation. Our results suggest a model in which structurally similar members of the same bZIP superfamily differ significantly in their regulation at the level of dimerization.
![]() |
MATERIALS AND METHODS |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Expression and Purification of His6-CREB
The His6-CREB fusion protein was constructed by
inserting the entire rat CREB-1 cDNA (9) together with a histidine
tag into the pET22b expression vector (Novagen, Madison, WI). The histidine tag, comprising six consecutive histidines
(His6), was located at the 5' terminus of the coding
region. BL21(DE3) cells (Novagen, Madison, WI) were transformed with
the plasmid containing the fusion proteins, and expression of the
protein was induced by treatment with 600 µM
isopropyl-1-thio--D-galactopyranoside. Bacterial cells
were harvested and lysed by lysozyme followed by sonication in binding
buffer (20 mM
Na2HPO4-NaH2PO4, 500 mM NaCl, 20 mM imidazole, 10 mM
2-mercaptoethanol, pH 7.8). The cell extract was clarified by
centrifugation and then directly applied to metal-chelated affinity
resin (Novagen, Madison, WI) charged with Ni2+. The bound
His6-CREB was eluted by a stepwise increase in imidazole from 50 to 500 mM. Purified His6-CREB, which
eluted at 200 mM imidazole, was concentrated under
N2 by an ultrafiltration apparatus (Amicon, Beverly, MA)
with a 30-kDa molecular mass cut-off filter. The concentrated protein
was dialyzed with 20 mM Hepes, 100 mM KCl, 10 mM dithiothrietol (DTT), pH 7.5.
Phosphorylation of His6-CREB
Phosphorylation of His6-CREB utilized the catalytic subunit of PKA (Promega, Madison, WI). A ratio of 1 unit of enzyme/5 pmol of protein was employed in each phosphorylation reaction. Samples were incubated with PKA at 30 °C for 15 min in 20 mM Hepes buffer (pH 7.5), 100 mM KCl, 10 mM DTT after the addition of 1 mM ATP, 3 mM Mg2+. The presence of phosphorylated CREB was verified by antibody detection using anti-phospho-CREB antibody (see below). The extent of phosphorylation was determined by gel mobility shift analysis using 32P-end-labeled 23-bp somatostatin CRE as a probe (see "Gel Mobility Shift Analysis"), the incorporation of [32P]ATP, and two-dimensional electrophoresis.
Antibody Detection of His6-CREB and P-His6-CREB
For detecting His6-CREB and/or P-His6-CREB, samples (20 µl) were separated on 12% SDS-polyacrylamide gels (16 cm × 16 cm × 1 mm) (SDS-PAGE) (36) at a constant current of 24 mA. After separation, the gel was transferred (in 39 mM glycine, 48 mM Tris, 0.04% SDS, 20% methanol) onto nitrocellulose membrane. The His6-CREB or P-His6-CREB could be detected by incubation with rabbit anti-CREB polyclonal antibody (CREB-1(240); Santa Cruz Biotechnology, Inc., Santa Cruz, CA), which detects both forms. P-His6-CREB could be distinguished from His6-CREB by detection with a rabbit anti-serum raised to the phosphorylated CREB (at Ser133) as described previously (20).
Gel Mobility Shift Analysis
The DNA binding activity of purified His6-CREB was examined by a gel mobility shift assay as described previously (20, 37). Oligonucleotide CRE probes included a 27-bp somatostatin CRE (5'-AGAGATTGCCTGACGTCAGAGA GCTAG-3'), a 26-bp Fos CRE (5'-TTCCGCTCATGACGTAGTAAGCCATT-3'), and a 23-bp dynorphin CRE1 (5'-GTACCTGGTACGTCACAGACATT-3'). Included as a negative control was a 26-bp AP-2 (5'-GATCGAACTGACCGCCCGCGGCCCGT-3') oligonucleotide. Roughly 6.4 µg of His6-CREB was incubated with 2.5 µg of salmon sperm DNA in gel shift buffer at room temperature for 5 min prior to the addition of 1 pmol of 32P-end-labeled probe. Complexes were analyzed by 5% nondenaturing polyacrylamide gel (37).
Filter Binding Assay
Constant amounts of 32P-labeled 23-base pair duplex
oligonucleotides or 23-mer strands containing the specific binding site (37, 38) were equilibrated with CREB proteins in binding buffer (37).
The concentration of CREBs varied over 3-4 orders of magnitude. Each
complex was gently filtered through nitrocellulose filters to obtain
the fraction bound. Radioactivity retained on the filter was detected
on a -scanner (Ambis). Dissociation constants were calculated from
the concentration of CREB protein that achieved half-maximal saturation
for the specific site [CREBtotal]1/2 = KD + (1/2)[DNAtotal] (39).
Cell Culture and Transient Assays
African green monkey kidney cells CV-1 were grown in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum. Plasmids were introduced into cells by calcium phosphate precipitation followed by glycerol shock (38). Transient transfection studies utilized a CREB or His6-CREB expression vector, a somatostatin-CAT reporter plasmid, and a PKA expression vector that have been previously described (37, 38). The CRE-containing somatostatin-CAT reporter was introduced into CV-1 cells with 5 µg of vector expressing native CREB, His6-CREB, or PKA catalytic subunit as indicated.
Sedimentation Equilibrium
Analysis of Free Protein--
Sedimentation equilibrium
measurements were performed on a Beckman model E analytical
ultracentrifuge equipped with interferometric optical system or on a
Beckman Optima XL-A equipped with a UV-visible detection system
(Beckman Instruments, Palo Alto, CA). Solutions of
His6-CREB or P-His6-CREB ranged from 0.5 to
16.0 µM, and the reference used in all experiments was
the dialysis buffer of the sample (see "Expression and Purification
of His6-CREB"). Double sector cells (30-mm height with
12-mm pass length) were used in experiments. Generally, 200 µl of
His6-CREB and P-His6-CREB were loaded into the
sample sector, and 215 µl of dialysate was added into the reference
sector of each cell. At room temperature, equilibrium was achieved at
24 h when the rotor speed was 12,000 rpm. The partial specific
volume (o) of His6-CREB was calculated to be
0.72 ml/g based on its amino acid sequence and the partial specific
volume of each amino acid (
i) (40). Utilizing the
interferometric optical system, the interferogram was acquired on
technical pan film (Eastman Kodak Co.). The distance (r)
from the middle of each fringe to the axis of the centrifuge was
determined by adding the distance from the middle of each fringe to the
wire of the cell under the microcomparator and the distance from the axis to the wire of the cell (provided by the manufacturer). Utilizing the absorbance optical system, protein boundaries were monitored at 258 nm, the wavelength that gives maximal difference in absorbance between
the sample and the reference. Molecular weights for one or two
components were obtained from the best fit of the data using the
nonlinear Levenberg-Marquardt fitting routine (41) according to the
equation,
![]() |
(Eq. 1) |
Analysis of DNA-Protein Complexes by Fluorescein End Labeling of
DNA--
For DNA-protein complexes, His6-CREB and
P-His6-CREB were incubated with 1.0-2.5 µM
somatostatin CRE (5'-GCCTCCTTGGCTGACGTCAGAGAGAG-3') or dynorphin CRE3
(5'-GTGGCTGCTGCGTCAGAGCATGA-3') that was end-labeled with fluorescein
(42). Complexes were formed after incubation of protein with DNA
overnight at room temperature. All experiments were carried out at
20 °C and at speeds between 8,000 and 20,000 rpm until equilibrium
was achieved. Equilibrium boundaries were detected by scanning the
absorbance across the cell at 494 nm, the maximum absorbance wavelength
for fluorescein. The use of a fluorescein tag on the DNA allows
analysis of DNA-protein complexes without interference from free
protein or DNA as long as the DNA is fully bound. Neither
His6-CREB nor P-His6-CREB absorbs at 494 mm.
Boundaries were analyzed as described above using relevant partial
specific volumes. The o value of the fluorescein-labeled CRE-containing oligonucleotides were calculated by inverting DNA effective density (
) in salt solution (43). Since average DNA
is
about 1.7 g/ml (44),
o of the DNA is 0.588 ml/g (
o = 1/
). The partial specific volumes of protein-DNA complexes were estimated by the equation,
![]() |
(Eq. 2) |
Gel Filtration
Purified His6-CREB (6 ml) was applied to a 2.5 × 110-cm2 Sephacryl S-200 HR column that had been packed
and equilibrated with Hepes buffer (20 mM Hepes, 100 mM KCl, 10 mM DTT, pH 7.5). The column was
calibrated by blue dextran 2,000 and protein molecular mass standards
between 13.7 and 232 kDa (Amersham Pharmacia Biotech). Void volume
(o) of the column was 203 ml. Proteins were eluted at a flow
rate of 1.5 ml/min and collected as 4-ml aliquots. Fractions were
concentrated 40-fold by a Centricon-30 concentrator (Amicon, Beverly,
MA) at 4 °C and were analyzed by antibody detection using a rabbit
anti-CREB Ab as described under "Antibody Detection of
His6-CREB and P-His6-CREB."
Limited Proteolysis and Peptide Sequencing
The His6-CREB-DNA complexes were formed by incubation of 1.1 nmol of His6-CREB or P-His6-CREB with 2.4 nmol of 27-bp somatostatin oligonucleotide at room temperature for 1 h. For limited proteolysis reaction, His6-CREB and P-His6-CREB or their DNA complexes were incubated with trypsin at a weight ratio of 100:1 (protein:trypsin). Since P-His6-CREB samples contained some PKA (which is also a substrate for trypsin), PKA was also added to the CREB samples. However, ATP was not included in these reactions to prevent phosphorylation. The reactions were incubated at 37 °C and terminated by the addition of SDS sample buffer, and proteolytic fragments were separated on 15% SDS-PAGE gel. For amino acid sequencing, proteolytic products were transferred by electroblotting from SDS-PAGE separating gel to polyvinylidene difluoride membrane (Millipore, Bedford, MA) in transfer buffer (190 mM glycine, 25 mM Tris, 15% methanol). After transfer, bands on the membrane were visualized by staining with a Coomassie Brilliant Blue R250 solution (0.1% Coomassie Brilliant Blue R250, 40% methanol, 1% glacial acetic acid). Each proteolysis band was excised and sequenced by Edman degradation (pulsed liquid method) in a protein sequencer (Applied Biosystems 492 Precise Protein Sequencing System, Perkin-Elmer).
![]() |
RESULTS |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
His6-CREB Fusion Protein Functions Normally-- The His6-CREB is purified almost to homogeneity in 200 mM imidazole elution from an Ni2+-charged affinity column (Fig. 1A). Because the histidine tag adds 33 amino acids to native CREB-1, the purified fusion protein migrates more slowly on SDS-PAGE compared with native CREB (Fig. 1B). That the protein band is His6-CREB is confirmed by antibody detection (Fig. 1B) and by its ability to specifically bind to CRE-containing oligonucleotides (Fig. 1C). Further, the His6-CREB fusion protein was efficiently phosphorylated by PKA (Fig. 1, D and E). In previous studies, we had found that the DNA-protein complex containing phospho-CREB migrates more slowly than CREB under gel shift conditions. After modification by PKA, we find that the entire His6-CREB-shifted band migrates more slowly relative to complexes of unmodified His6-CREB (Fig. 1D). We confirmed that greater than 95% of the His6-CREB is modified by PKA phosphorylation using isolelectric focusing (data not shown) and incorporation of radiolabeled ATP. All of the modified CREB is detected by an antibody that recognizes only a Ser133-phosphorylated form of CREB (20) and a regular anti-CREB-1 antibody (Fig. 1E). Taken together, these data suggest that our preparation of His6-CREB and P-His6-CREB represents a pure population of each form, suitable for physical studies.
|
|
The Apparent Binding Constant of CREB Is Not Changed by PKA-dependent Phosphorylation-- To quantify the effects of phosphorylation on the binding affinity of CREB in solution, we measured the dissociation constants of CREB and P-CREB by filter binding. We observed no change in the apparent KD among the different templates containing both symmetric and asymmetric CREs (compare Som CRE and Dyn CRE-1; Fig. 2B). Binding is, however, CRE-dependent, since the dissociation constant is small for CRE-containing oligonucleotides (Som CRE and Dyn CRE-1; Fig. 2B) and larger for non-CRE containing template (Enk AC; Fig. 2B) or for a point mutation (Dyn CRE-1.2; Fig. 2B) that abrogates CREB binding to dynorphin CRE-1 in gels (38). For P-CREB, the binding constant is indistinguishable from CREB for any template examined (Fig. 2B). We conclude that the apparent binding constant of CREB is not changed by PKA-dependent phosphorylation regardless of the symmetry of the CRE region.
Dimerization of CREB Is DNA-dependent-- The apparent dissociation constants were calculated based upon an assumption that a preformed CREB dimer binds to the CRE in both unphosphorylated and phosphorylated forms. Many nonsolution studies using truncated or mutant molecules and gel shift analysis indicate that dimers of unphosphorylated CREB bind to a CRE (33, 45). However, whether CREB remains as a dimer after phosphorylation has not been directly examined. If phosphorylation by PKA alters the self-association state of CREB, then the binding constants will be affected. To determine the form of CREB both on and off DNA in solution, we measured the self-association state of CREB and P-CREB by sedimentation equilibrium analysis. The advantage of the method is that the molecular mass of a macromolecule or macromolecular complex can be directly measured in solution. The concentration of CREB in PC-12 cells has been estimated to be roughly 0.4 µM (46). Based on this estimate, concentration conditions used for the experiment were selected to be near physiological, ranging between 0.5 and 16.0 µM.
We found that both CREB and P-CREB free in solution are primarily monomers in a concentration range from 0.5 to 16.0 µM (Fig. 3A). The predicted molecular mass of CREB from rat is 36.6 kDa (9), and the addition of the histidine tag increases the molecular mass to 40.4 kDa. The best fit to the concentration gradient reveals a major form with a molecular mass very close to the predicted mass of a monomer (Fig. 3A). Some amount of dimer and aggregation were apparent in the sample. Since the analysis yields a "weight average" molecular mass, the presence of some aggregated material skews the fitted value toward a slightly higher mass. Despite the error, it is clear from the data that CREB is not a dimer (80.8 kDa) under near-physiologic concentrations. Gel filtration chromatography confirms the results of the sedimentation experiment. The major eluate co-migrated with the 43-kDa protein standard, indicating that the monomer was the predominant form (Fig. 3B). We also detected a small amount of dimer (Fig. 3B) and some high molecular weight aggregation (not shown). The profile of free CREB was similar to that of free P-CREB (not shown). Thus, PKA-mediated phosphorylation does not promote dimerization of the free protein.
|
|
|
Dimerization of CREB at the CRE Site Is Cooperative-- There are two possible mechanisms by which CREB monomers can assemble on a DNA template. Each has biological significance. Monomers of CREB may assemble cooperatively on DNA to form dimers. In this case, a fraction of CRE binding sites may be unoccupied while others bind dimers. In a cooperative model, little to no CREB monomer should be bound at any ratio of CREB to DNA (Fig. 6A). Alternatively, CREB monomers may bind in a noncooperative manner, filling CRE sites as monomers before CREB dimers are formed (Fig. 6A). In the noncooperative model, a CREB monomer has equal probability to bind to a free CRE site or to a CREB monomer-occupied site. Therefore, at a low protein:DNA molar ratio, the major form in solution should be a monomer-DNA complex. However, with the increase of the ratio of protein to DNA, we expect that the fraction of the dimer-DNA complex should increase (Fig. 6A).
|
![]() |
DISCUSSION |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Many studies have led to the understanding that CREB is a dimer when bound to DNA (20, 33, 45). However, how CREB dimerizes or the effect of phosphorylation on the dimerization state has not been examined in solution. CREB binding has been analyzed largely using gel shift assays and other nonsolution techniques that cannot reliably distinguish between dimer and monomer states. Here we directly measure the effect of phosphorylation on CREB dimerization in solution. Our results reveal several novel properties of CREB dimerization and clarify aspects of DNA binding that have been previously reported.
First, free CREB does not homodimerize. Although CREB has never been crystallized, it has been suggested that dimerization of CREB is like that of Jun and Fos based on structural similarities of these bZIP family members (12, 49). Both solution (50) and x-ray diffraction (21) studies have shown that Jun and Fos dimerize on or off DNA as Jun-Jun or Jun-Fos complexes (21, 50). However, we find that, unlike Jun-Fos or Jun-Jun complexes, CREB is largely a monomer in solution. CREB indeed dimerizes at a CRE binding site but dimerization is DNA-dependent. Detailed spectroscopic and kinetic studies of bZIP peptides had suggested that CREB might bind to DNA as a monomer (34). Our results for the first time directly demonstrate this is the case for native CREB. Further, monomeric forms of bZIP family members may play a significant role in transcriptional activation. For example, SKN-1, a Caenorhabditis elegans transcriptional factor, binds DNA with high affinity as a monomer through its basic region, which is very similar to that of bZIP proteins (51, 52). Thus, dimerization may not be required for some bZIP proteins to either bind DNA or activate transcription. Structurally similar members of the bZIP family are very different with respect to dimerization.
The rules that govern dimerization are not fully understood. Many studies have correlated structural features of interfaces among leucine zipper proteins with their dimerization potential. Association between the two zippers is primarily due to van der Waals forces between the hydrophobic residues at the a and d positions in the helix (12, 53). Because of the structure and size, the presence of leucine at the d positions allows most stable packing at the dimer interface (54) and promotes formation of coiled-coils with a dimer of parallel helices. The hydrophobic positions a are much less conserved than positions d, although alanine substitutions at some a positions can be destabilizing (23). Additionally, the interface of the dimer is reinforced by interhelical salt bridges between polar residues at positions e and g (12). Dimerization is weakened if there is electrostatic repulsion between these positions. The electrostatic force between the interhelical e and g positions has been used to explain why Fos does not homodimerize (55, 56). CREB has a typical leucine zipper region in which the d positions are occupied exclusively by leucine and the a positions are generally occupied by valine or leucine. Overall, the e and g residues in CREB-1 are nearly neutral (12). Based on structural features of its leucine zipper interface, the lack of a significant CREB homodimer in solution is difficult to explain. However, residues outside the leucine zipper region also contribute to dimer stability (56-58), and the CREB protein may contain residues beyond the leucine zipper region that are important for dimer destabilization. Whatever the constraints, DNA binding clearly leads to dimerization. It remains to be determined whether bound CREB dimer forms a canonical leucine zipper interface or whether monomer binding simply facilitates the association of a second molecule.
Second, our results directly demonstrate that the binding constant of CREB does not need correction for alterations in its "form". Several groups have previously reported that PKA does not influence the apparent binding constant of CREB. However, in all other studies, dimerization after CREB phosphorylation was assumed but not tested. Here, we directly show that CREB cooperatively assembles on DNA to form a dimer and that dimerization is unaffected by PKA-dependent phosphorylation. Using nuclear or cellular extracts, PKA has been reported to increase the binding affinity of CREB particularly at nonsymmetric CRE sites (32). Based on our results, an increase in CREB binding under these conditions is likely to involve other dimerization partners. In this regard, it is significant that CREB binds cooperatively to the CRE. Cooperative binding allows greater regulation of transcription, since the monomer can receive signals that may influence the choice of dimer partner, which, in turn, attracts adapter proteins such as CBP. It has been reported that CREB can dimerize with ATF-1 (59) and CREM (60). Phosphorylation may control selection of a CREB dimerization partner. We have recently found that, in contrast to PKA, calmodulin kinase II phosphorylation abrogates CREB-dependent transcriptional activity and CREB dimerization on the prodynorphin CRE.2 Our results suggest that CREB phosphorylation at positions 133 and 142 by calmodulin kinase II may promote the association of a "repressive" dimer partner. Alternatively, CBP may not bind to the monomer if phosphorylation at 133 and 142 inhibits the ability of CREB to dimerize.
Finally, our studies suggest that dimerization properties may distinguish the roles of structurally similar bZIP proteins. Our results show that free CREB exists primarily as a monomer in the cell but cooperatively binds to DNA as a dimer. Neither DNA binding activity nor the self-association state of both free and DNA-bound CREB are regulated by PKA-induced phosphorylation regardless of the symmetry of the CRE site. In contrast, Jun and Fos proteins readily dimerize in solution and bind to DNA as a dimer. CREB is a constitutive protein in the cell, while expression of Jun and Fos are induced in response to external stimuli. Jun and Fos dimerize for immediate function but are not always present in the cell. Thus, bZIP proteins of the CREB family are constitutively transcribed but are regulated at the level of dimerization. In contrast, Jun and Fos are regulated at the level of transcription but are designed to constitutively dimerize for immediate function. Structurally similar members of the same superfamily appear to differ significantly in their regulation at the level of dimerization.
![]() |
ACKNOWLEDGEMENTS |
---|
We thank Debbie S. Schwerman for preparing the manuscript and Pamela S. Mertz for assistance with the transient CAT assays.
![]() |
FOOTNOTES |
---|
* This work was supported by the Mayo Foundation, National Institutes of Health Grant DK 43694-01A2 (to C. T. M), National Science Foundation Grant IBN 9222848 (to C. T. M), and the Levy Foundation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
¶ To whom correspondence and reprint requests should be addressed: Dept. of Pharmacology, Mayo Foundation, 200 First St., S.W., Rochester, MN 55905. Tel.: 507-284-1597; Fax: 507-284-9111; E-mail: mcmurray.cynthia{at}mayo.edu.
The abbreviations used are: CREB, cAMP response element-binding protein; CRE, cAMP-responsive element; PKA, cAMP-dependent protein kinase; CBP, CREB-binding protein; DTT, dithiothreitol; bp, base pair(s); PAGE, polyacrylamide gel electrophoresis; CAT, chloramphenicol acetyltransferase.
2 X. Wu, unpublished observations.
![]() |
REFERENCES |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|