From the Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109
![]() |
ABSTRACT |
---|
![]() ![]() ![]() ![]() ![]() ![]() |
---|
The apoptotic machinery of Caenorhabditis elegans includes three core interacting components: CED-3, CED-4, and CED-9. CED-3 is a death protease composed of a prodomain and a protease domain. CED-4 is a P-loop-containing, nucleotide-binding molecule that complexes with the single polypeptide zymogen form of CED-3, promoting its activation by autoprocessing. CED-9 blocks death by complexing with CED-4 and suppressing its ability to promote CED-3 activation. A naturally occurring alternatively spliced form of CED-4 that contains an insertion within the nucleotide-binding region (CED-4L) functions as a dominant negative inhibitor of CED-3 processing and attenuates cell death. Domain mapping studies revealed that distinct regions within CED-4 bind to the CED-3 prodomain and protease domain. Importantly, the CED-4 P-loop was involved in prodomain binding. Disruption of P-loop geometry because of mutation of a critical lysine (K165R) or insertional inactivation (CED-4L) abolished prodomain binding. Regardless, K165R and CED-4L still retained CED-3 binding through the protease domain but were unable to initiate CED-3 processing. Therefore, the P-loop-prodomain interaction is critical for triggering CED-4-mediated CED-3 processing. Underscoring the importance of this interaction was the finding that CED-9 contacted the P-loop and selectively inhibited its interaction with the CED-3 prodomain. These results provide a simple mechanism for how CED-9 functions to block CED-4-mediated CED-3 processing and cell death.
![]() |
INTRODUCTION |
---|
![]() ![]() ![]() ![]() ![]() ![]() |
---|
Tissue homeostasis in multicellular organisms depends upon appropriately regulated programmed cell death (1-4). Disruption of this physiologic process, termed apoptosis, contributes to the pathogenesis of several human diseases (5-7). Apoptosis is evolutionarily conserved and genetically regulated (8). The genetic dissection of developmental cell death in the nematode Caenorhabditis elegans has illuminated three core components of the cell death pathway: CED-3, CED-4, and CED-9 (9). Although CED-3 and CED-4 induce cell death, CED-9 is a negative regulator and inhibits apoptosis.
Mammalian counterparts to the worm components include the Bcl-2 family that is related to CED-9 (10, 11), the caspase family of proteases that are similar to CED-3 (12), and the recently identified Apaf-1 molecule that is like CED-4 (13-15). The interchangeability of death components between worm and man emphasize their conservation and suggest that they likely share a fundamentally similar mechanism of action. For example, CED-3 transfected into mammalian cells will effectively activate endogenous caspases leading to cell death (16). Conversely, ced-9 loss of function worm mutants can be partially complemented by human Bcl-2 (10). Although similar in outline, the mammalian pathway is complex in that each family has a number of distinct gene products. The Bcl-2 family, for example, has upwards of 16 members (17-21), some of which, like CED-9, function to inhibit cell death, whereas others promote cell death. Given that the basic mechanism of action is likely conserved, we have sought to understand how the core components of the worm death machine function as a means of illuminating the underlying biochemistry of mammalian cell death.
CED-4 functions by binding CED-3 and facilitating its proteolytic autoactivation from the zymogen form to the active dimeric species (16, 22-26). Although CED-4 itself is not a protease, it contains a phosphate-binding P-loop motif and a magnesium-binding site that is observed in nucleotide-binding proteins, including ATPases (26). Mutation of the P-loop motif inhibits the ability of CED-4 to activate CED-3 (16, 26). Additionally, CED-4 can be photoaffinity labeled by the ATP analogue 8-N3ATP (26).1 Further, the noncleavable ATP analogue FSBA blocks CED-4-mediated activation of CED-3 (26), indicating a requirement for ATP hydrolysis. Collectively, these data indicate that CED-4-mediated activation of CED-3 requires an intact nucleotide binding capability and can be extrapolated to suggest that CED-4 functions as an ATPase. Native CED-4 is capable of simultaneously binding the proapoptotic CED-3 zymogen and the anti-apoptotic CED-9 molecule to form a neutral ternary complex (16, 23-25, 27, 28). In this complex, CED-4 bound to CED-9 is still able to bind CED-3 but is unable to stimulate its processing. Intriguingly, CED-4L contains an in-frame 72-base pair insertion between the P-loop and Mg2+-binding site (29) that should disrupt the architecture of the nucleotide-binding domain. In keeping with this notion, CED-4L could not be affinity labeled using an azido-ATP analog.2 A more comprehensive description of the binding regions involved was obtained by undertaking a deletional mapping study that unexpectedly provided mechanistic insight into how CED-4L and CED-9 abrogate cell death.
![]() |
EXPERIMENTAL PROCEDURES |
---|
![]() ![]() ![]() ![]() ![]() ![]() |
---|
Cells and Transfections-- Epitope-tagged expression constructs were made in pcDNA3 or pcDNA3.1/MycHisA (Invitrogen) using standard recombinant methods. 293T cells were transiently transfected with the indicated expression plasmids using the calcium phosphate method as described previously (16). The catalytic mutant (CED-3mt) was utilized as wild type CED-3 activated the apoptotic program upon overexpression. Cells were harvested 18-42 h following transfection for immunoprecipitation or protein purification.
Co-immunoprecipitation-- Immunoprecipitations were performed 24-38 h following transfection essentially as described previously (16). Briefly, cells were harvested and lysed in 1 ml of lysis buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA, 1% Nonidet P-40, and protease inhibitors). The lysates were divided equally and immunoprecipitated with control or specific antibodies as indicated for 2 h at 4 °C. The beads were washed with lysis buffer (adjusted to 500 mM NaCl) three times and transferred to nitrocellulose. Subsequent protein immunoblotting was performed as described previously (16).
In Vitro Binding Analysis-- CED-4 constructs were expressed in 293T cells and purified by immunoprecipitation and immobilization on protein G beads, followed by 2 h of incubation with 35S-labeled CED-3 at 4 °C. The samples were washed extensively in 500 mM NaCl buffer as described above and analyzed by SDS-polyacrylamide gel electrophoresis, followed by fluorography and autoradiography.
In Vitro Processing of CED-3-- Extracts were prepared from 293T cells 36 h following transfection with the indicated expression constructs. Cells were incubated in cold hypotonic buffer containing protease inhibitors. The swollen cells were lysed with 100 strokes in a Dounce homogenizer, and the lysates were clarified by centrifugation at 4 °C, 20,900 × g. 35S-Labeled CED-3 was synthesized by in vitro transcription/translation (Promega) (30 min, 30 °C). Prolonged incubation of the in vitro translate triggers auto-processing of CED-3 (30) and was therefore avoided. The processing reactions were performed using 5 µl of S-labeled CED-3 and 20 µl of lysate containing the indicated proteins and incubated for 1 h at 30 °C. The processing products were resolved by SDS-polyacrylamide gel electrophoresis and visualized by autoradiography.
Subcellular Localization-- Epitope-tagged expression constructs were transfected in 293T cells using calcium phosphate. The cells were analyzed 24 h following transfection. Briefly, cells were incubated with antibodies against the indicated epitopes for 1 h at 23 °C, followed by incubation with fluorescein isothiocyanate-conjugated (Sigma) or Cy3-conjugated secondary antibodies (Sigma) for 1 h at 23 °C. The cells were washed and mounted in Prolong Antifade (Molecular Probes) and analyzed by confocal microscopy (Bio-Rad MRC 600 scanning confocal microscope).
![]() |
RESULTS AND DISCUSSION |
---|
![]() ![]() ![]() ![]() ![]() ![]() |
---|
Deletion of the P-loop Motif Disrupts CED-4-CED-3 Prodomain Binding-- CED-4 deletion mutants (Myc-His or HA epitope-tagged) were co-expressed in 293T cells with FLAG epitope-tagged CED-3. The lysates were divided equally for immunoprecipitation with control or specific antibodies followed by Western blot analysis for CED-4 proteins co-precipitating with CED-3. Because CED-4269-549 did not bind CED-3 but C-terminal truncations containing amino acid residues 1-269 co-precipitated with CED-3 (Fig. 1, A-E), this segment contained the CED-3-binding site. Further, because the N-terminal truncation CED-4171-549 bound CED-3 (Fig. 1B), an interaction site could be localized to residues 171-269 of CED-4. However, because CED-41-152 also co-precipitated with CED-3 (Fig. 1D), two distinct CED-3 interaction domains must exist within the N-terminal half: residues 1-152 and 171-269. These domain interactions are also observed in an in vitro binding analysis of 35S-labeled CED-3 with CED-4171-549 and CED-41-152 (Fig. 1F).
|
|
CED-4-mediated CED-3 Processing Requires the P-loop-Prodomain Interaction-- Because both CED-4L (16) and the P-loop inactivating point mutant K165R (Fig. 1E) bound CED-3 but did not initiate its processing (26), we hypothesized that this was because of lack of the crucial P-loop-prodomain interaction. In such a scenario, the observed binding would be mediated exclusively through the protease domain. Consistent with this, CED-4K165R, as well as CED-4L, bound the CED-3 protease domain but failed to interact with the CED-3 prodomain (Fig. 3A).
|
CED-9 Selectively Disrupts the CED-3 Prodomain Binding to CED-4-- In the presence of CED-9, a ternary complex is assembled with CED-4 still bound to CED-3 (Fig. 4A), but no longer able to trigger its processing (16, 22-26, 27, 28). To define the mechanism by which CED-9 accomplishes this, we asked if, in the CED-3-CED-4-CED-9 ternary complex, the crucial prodomain-P-loop interaction was disrupted. As anticipated, full-length CED-3 and CED-3 protease domain co-precipitated with CED-4 complexed to CED-9 (Fig. 4A), but CED-3 prodomain did not (Fig. 4A). This is consistent with CED-9 inhibiting binding of the P-loop to the prodomain. Such an inhibition could be the result of steric hindrance, especially if CED-9 also bound the P-loop (22). Consistent with this, CED-9 was found to bind native CED-4 but not CED-4171-549, which lacks the P-loop motif or K165R, the P-loop inactivating point mutant (Fig. 4B). Regardless, disruption of the P-loop-prodomain interaction within the ternary CED-3-CED-4-CED-9 complex likely accounts for the mechanism by which CED-9 neutralizes the ability of CED-4 to activate CED-3.
|
CED-3, CED-4, and CED-9 Form a Ternary Complex in Cells-- To confirm that the three core components can indeed form a ternary complex in vivo consistent with the biochemical data (16) each component was expressed individually or together in 293T cells. The subcellular localization was determined by immunostaining and confocal microscopy. When expressed alone in mammalian cells, CED-9 displayed a compact granular pattern confined to the perinuclear region and membranes of intracellular organelles (presumably mitochondria) (Ref. 35; Fig. 5A). By contrast, CED-4 exhibited a diffuse cytoplasmic labeling, whereas CED-3 stained a punctate cytoplasmic pattern reminiscent of bacterial inclusion bodies and suggesting insolubility (Fig. 5A). Although recent studies have shown that CED-9 can redistribute the subcellular localization of CED-4 from cytosolic to intracellular membranes (35), we show for the first time that CED-3-CED-4-CED-9 can assemble as a ternary complex at intracellular membranes (Fig. 5B).
|
Summary-- It has recently been shown that CED-9 can serve as a substrate for the CED-3 protease and that a competitive inhibition mechanism may in part account for the cell death inhibitory activity of CED-9 (36). Although competitive inhibition may be part of the mechanism by which CED-9 exerts its antiapoptotic effect, our studies support an active role within the CED-3-CED-4-CED-9 ternary complex in which CED-9 suppresses CED-3 activation by selectively inhibiting the prodomain-P-loop interaction.
![]() |
ACKNOWLEDGEMENTS |
---|
We thank the following members of the Dixit laboratory: I. Jones, Y. Kuang, H. Duan, M. Muzio, J. McCarthy, E. Humke, C. Vincenz, and S. Hu.
![]() |
FOOTNOTES |
---|
* This work was supported by National Institutes of Health Grant 07863.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Present address: 1 DNA Way, Genentech, Inc., South San Francisco,
CA 94080.
§ To whom correspondence should be addressed. Tel.: 650-225-1312; Fax: 650-225-6127; E-mail: dixit{at}gene.com.
1 The abbreviations used are: 8-N3ATP, 8-azidoadenosine-5'-triphosphate; FSBA, 5'-fluorosulfonyl benzoyladenosine; mAb, monoclonal antibody; HA, hemagglutinin.
2 D. Chaudhary and V. M. Dixit, unpublished observation.
![]() |
REFERENCES |
---|
![]() ![]() ![]() ![]() ![]() ![]() |
---|