Divergent Transcriptional Control of Multidrug Resistance Genes in Saccharomyces cerevisiae*

Timothy C. HallstromDagger and W. Scott Moye-RowleyDagger §

From the Dagger  Molecular Biology Program and the § Department of Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52242

    ABSTRACT
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Abstract
Introduction
Materials & Methods
Results
Discussion
References

Improper control of expression of ATP binding cassette transporter-encoding genes is an important contributor to acquisition of multidrug resistance in human tumor cells. In this study, we have analyzed the function of the promoter region of the Saccharomyces cerevisiae YOR1 gene, which encodes an ATP binding cassette transporter protein that is required for multidrug tolerance in S. cerevisiae. Deletion analysis of a YOR1-lacZ fusion gene defines three important transcriptional regulatory elements. Two of these elements serve to positively regulate expression of YOR1, and the third element is a negative regulatory site. One positive element corresponds to a Pdr1p/Pdr3p response element, a site required for transcriptional control by the homologous zinc finger transcription factors Pdr1p and Pdr3p in other promoters. The second positive element is located between nucleotides -535 and -299 and is referred to as UASYOR1 (where UAS is upstream activation sequence). Interestingly, function of UASYOR1 is inhibited by the downstream negative regulatory site. Promoter fusions constructed between UASYOR1 and the PDR5 promoter, another gene under Pdr1p/Pdr3p control, are active, whereas analogous promoter fusions constructed with the CYC1 promoter are not. This suggests the possibility that UASYOR1 has promoter-specific sequence requirements that are satisfied by another Pdr1p/Pdr3p-regulated gene but not by a heterologous promoter.

    INTRODUCTION
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Abstract
Introduction
Materials & Methods
Results
Discussion
References

Mammalian tumor cells can acquire the ability to detoxify several unrelated cytotoxic agents through alteration of a small number of genetic loci. This phenomenon, termed multidrug resistance, often involves the increased expression of an ATP binding cassette (ABC)1 transporter protein known as MDR1 (reviewed in Ref. 1). Recently, overexpression of a second ABC transporter protein has been shown to also confer a multidrug-resistant phenotype on cells. This membrane transporter protein was designated the multidrug resistance protein (Mrp) (2) and later shown to be responsible for production of a glutathione S-conjugate transporter activity in hepatocytes (3, 4). Control of the levels of expression of these mammalian ABC transporters is a critical determinant in the relative drug tolerance of an animal cell.

The yeast Saccharomyces cerevisiae also contains loci that can be altered to give rise to multiple or pleiotropic drug resistance (reviewed in Ref. 5). Dominant mutations in the Cys6 zinc finger transcription factors PDR1 and PDR3 result in elevated resistance to a broad array of toxic agents including cycloheximide and oligomycin (6-8). The ABC transporter protein-encoding gene PDR5 is transcriptionally activated by Pdr1p and Pdr3p and is required for Pdr1p/3p-mediated cycloheximide resistance (9, 10). Cells containing a Delta pdr5 allele are cycloheximide-hypersensitive but display no oligomycin sensitivity, indicating the presence of a second Pdr1p/3p target gene required to provide oligomycin resistance. This oligomycin resistance gene, YOR1, was subsequently cloned on the basis of its ability to strongly elevate tolerance to this compound when present on a high copy plasmid (11).

DNA sequence analysis of the YOR1 gene (11) demonstrated that this locus encodes a protein with striking sequence similarity to the human cystic fibrosis transmembrane conductance regulator (12), Mrp (2), and S. cerevisiae Ycf1p (13). YOR1 is transcriptionally regulated by Pdr1p and Pdr3p and inspection of the YOR1 promoter region indicated the presence of a potential Pdr1p/Pdr3p response element (PDRE) centered 215 bp upstream of the transcription start site (11). These PDREs have been defined previously by analysis of similar elements in the promoters of PDR3 (14), PDR5 (15), and SNQ2 (16, 17). Northern blot and lacZ gene fusion analysis showed that YOR1 retained significant expression in a Delta pdr1,3 strain (11). This is a marked difference from the behavior of the Pdr1p/Pdr3p-regulated PDR5 gene which is essentially inactive in a Delta pdr1,pdr3 mutant background, explaining the cycloheximide-hypersensitive phenotype of this strain (10). It was anticipated that YOR1 expression would also strongly depend on PDR1/PDR3 since a Delta pdr1,3 strain is extremely oligomycin-sensitive (10, 18).

To explore the nature of the differences between the co-regulated PDR5 and YOR1 promoters, we have carried out an analysis of the transcriptional control elements of YOR1. Deletion mutagenesis of a YOR1-lacZ gene fusion defined three regions important for normal regulation of YOR1 expression, two positively acting cis-elements and one negative regulatory site. One of the positive elements corresponds to the PDRE, confirming the role of this site in YOR1 transcriptional control. The second positive element is located upstream of the PDRE and is normally repressed by the action of the negative regulatory region. The activities of these other two elements appear to be independent of PDR1 and PDR3. These data are consistent with the YOR1 promoter receiving multiple regulatory inputs from PDR1/PDR3 and other as yet unknown factors.

    MATERIALS AND METHODS
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Abstract
Introduction
Materials & Methods
Results
Discussion
References

Yeast Strains and Media-- Yeast transformations were performed using the lithium acetate procedure (19). The previously described strains used in these studies are SEY6210 (MATalpha ; leu2-3,-112; ura3-52; his3-Delta 200; trp1-Delta 901; lys2-801 suc2-Delta 9; Mel-), PB4 (SEY6210; pdr1-Delta 2::hisG; pdr3-Delta 1::hisG) (10), and DKY7 (SEY6210; yor1-1::hisG) (11). TCH5 (SEY6210, yor1-Delta 1::hisG) was used for the RNase mapping experiments. Standard SD and YPD media (20) were used for normal yeast growth and determination of drug resistance. Drug resistance was assayed by spot testing on plates containing different drug concentrations (21). beta -Galactosidase assays were performed as described previously (22).

Primer Extension Analysis-- A YOR1-specific oligonucleotide was synthesized that corresponds to nucleotides +49 to +75 in the YOR1 gene. This oligonucleotide was labeled at its 5' end using [gamma -32P]ATP and T4 polynucleotide kinase. The radiolabeled oligonucleotide was then annealed to 50 µg of total RNA isolated using a rapid procedure (23) from strains of interest. Annealing was allowed to proceed for 45 min at 65 °C, then incubated for an additional 45 min at 45 °C, followed by reverse transcriptase extension of the RNA-DNA complexes. Primer extension was carried out for 1 h at 37 °C; the reaction was stopped by phenol extraction, and the nucleic acids were precipitated. Primer extension reactions were electrophoresed through a 6% polyacrylamide/urea gel in parallel with dideoxy sequencing reactions performed on a YOR1 template using the same oligonucleotide primer.

Plasmids-- The wild-type YOR1-lacZ plasmid pSM109-4 has been previously described (11). Briefly, pSM109-4 corresponds to a YOR1-lacZ gene fusion containing 1066 bp upstream of the transcription start site and 213 bp of coding sequence from YOR1. This same YOR1 DNA fragment was cloned into pBluescript KS II+ as a BamHI/EcoRI fragment to form pTH40. 5' deletions were generated by digesting pTH40 with EcoRI, treating this DNA with the bidirectional exonuclease Bal31, forming blunt ends with Klenow enzyme and deoxyribonucleotides, and then ligating an EcoRI linker to the resulting blunt-ended fragments. The ligation reaction was then cleaved with EcoRI/BamHI and cloned into similarly digested pSEYC102 (24). The 5' end of each deletion mutant was sequenced to precisely determine the 5' end point. An oligonucleotide (CCG GAA TTC GAA TAT GTC GAT TAC CGT GGG GGA T) was used to introduce an EcoRI site upstream of the YOR1 ATG in a PCR reaction, resulting in the plasmid pTH70. 3' promoter deletions were generated in the same fashion as the 5' deletion mutants, except pTH40 was cut initially with BamHI. These 3' deletions were cloned as EcoRI fragments into YOR1 5' deletion mutant constructs resulting in internal promoter deletions.

A clustered point mutation was introduced into the YOR1 PDRE by PCR mutagenesis as described (15). The plasmid pTH40 was PCR-amplified using the YOR1 primer (GGC TCT CTA CTA AAT GCT TGC G) and the T7 primer. A separate PCR reaction was performed on pTH40 using the mutagenic primer (ATT TCC GGC TAT gTC gAC GGA ACC ACG CGT TAT C) and the T3 primer. The mutant base pairs are indicated by lowercase letters. PCR products from these reactions were purified, combined, and re-amplified with the T3 and T7 primers. The product from this reaction was digested with Eco47III and BglII and cloned into Eco47III/BglII cut pTH40. Resulting plasmids were sequenced to verify that the clustered point mutation had been introduced into the PDRE and that no other mutations had been introduced.

The promoter fusion plasmids were constructed using either the CYC1-lacZ vector pCBS1 (25) or the PDR5-lacZ vector pPBDelta 112 (10). These plasmids contained versions of each promoter that replaced the normal upstream activation sequences with a unique EcoRI site. YOR1 promoter fragments were inserted as EcoRI fragments in their normal orientation relative to the heterologous promoters.

RNase Mapping Analysis of YOR1-lacZ Transcripts-- To detect the chimeric YOR1-lacZ transcripts with no interference from the chromosomal YOR1 gene, a strain was constructed that lacked the region of YOR1 around the transcription start site. This was accomplished by preparing a XhoI/HindIII subclone of YOR1 in pBluescript. An internal BglII fragment extending from -109 to +1129 was then replaced with the BamHI/BglII hisG-URA3-hisG fragment from pNKY51 to produce pTH140. A SacI/KpnI fragment of pTH140 was used to transform SEY6210 cells to URA3+. Appropriate integration was confirmed by Southern blotting (26). The resulting transformant was then treated with 5-fluoroorotic acid and used as host for the YOR1-lacZ fusion plasmids. This Ura- cell was designated TCH5. Total RNA was prepared from TCH5 transformants by hot phenol extraction (23). A YOR1-specific probe was generated by T7 transcription of BglII-linearized pTH40, and the PDC1 probe was produced by T3 transcription of a BamHI/SalI PDC1 subclone in pRS426 (27). The PDC1-containing plasmid was cleaved with BglII prior to in vitro transcription. RNase protection was carried out as described by the manufacturer (Ambion).

DNase I Footprint Analysis-- DNase I footprint analysis was performed as described (28). The vector pOTS-Nco12 (29) expressing a Myc-epitope tagged version of the N-terminal 248 amino acids of Pdr1p was used to produce protein for these experiments. This plasmid was constructed by inserting an oligonucleotide encoding the Myc epitope into the NcoI site of a pOTS-Nco12 derivative expressing the N-terminal 248 residues of Pdr1p (provided by Laurence Lambert).

    RESULTS
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Abstract
Introduction
Materials & Methods
Results
Discussion
References

Primer Extension Mapping of the YOR1 Transcription Start Site-- Previously, we localized the YOR1 transcription start site using an RNase protection assay (11). This assay provided an approximation of the site for transcription initiation accomplished by comparing the protected RNA species with an end-labeled set of DNA fragments. To locate precisely the YOR1 transcription start site, primer extension analysis was carried out. Total RNA was isolated from a wild-type strain transformed with the vector pRS315 or with pRS315 containing a dominant, gain-of-function allele of PDR1 designated PDR1-6. This gain-of-function PDR1 allele produced both elevated cycloheximide and oligomycin tolerance in addition to increasing expression of PDR5 and YOR1 (11, 30).

A 25-nucleotide YOR1-specific primer was radiolabeled with [gamma -32P]ATP and T4 polynucleotide kinase and then annealed to the RNA samples. This oligonucleotide was also used for dideoxy sequencing of a YOR1 template to generate a size standard for the primer extension products. The reverse transcription reaction products and the sequencing reactions were electrophoresed in parallel on a DNA sequencing gel (Fig. 1). These analyses generated two major products, sized 74 and 70 nucleotides. The 74-nucleotide extension product mapped to 185 nucleotides upstream of the first ATG codon in the YOR1 open reading frame and was designated as the transcription start site. This transcription start site agreed with separate primer extension analysis using a 25-base pair oligonucleotide spanning the YOR1 ATG (data not shown). As predicted from previous Northern blot and RNase protection analysis (11), YOR1 mRNA levels were elevated in the presence of PDR1-6. Having accurately located the start site for YOR1 transcription, we next carried out deletion analysis of the promoter region.


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Fig. 1.   Primer extension mapping of the YOR1 mRNA 5' end. Equal amounts of total RNA isolated from a wild-type strain (SEY6210) carrying a low copy plasmid (pRS315) or the same plasmid containing a PDR1 gain-of-function mutation (PDR1-6) were processed for reverse transcription using a YOR1-specific primer as described under "Materials and Methods." Primer extension was also performed on yeast tRNA (tRNA) as a control for primer specificity. The dideoxy sequencing reactions are labeled by the identity of the input dideoxy terminator used in each lane. The deduced position of the most upstream of the two major YOR1 transcription start sites is indicated by the arrow above the relevant YOR1 sequence.

5' Deletion Mapping of the YOR1 Promoter-- Low copy plasmids carrying the YOR1-lacZ fusion with progressively larger 5' promoter deletions were transformed into a wild-type strain or an isogenic Delta pdr1,3 strain. YOR1-lacZ-dependent beta -galactosidase activities were determined for each construct (Fig. 2).


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Fig. 2.   5' deletion analysis of the YOR1 promoter region. Each construct was transformed into either wild-type cells or a strain lacking both PDR1 and PDR3 (Delta pdr1,3). Selected transformants were grown in SD medium plus necessary supplement, and beta -galactosidase activity was determined as described (22). Values represent the average of at least five independent determinations ± S.D. The numbers refer to the nucleotide position relative to the transcription start site of YOR1. PDRE denotes the location of the Pdr1p/Pdr3p-binding site. The leftward arrow marks the position of the next recognizable upstream open reading frame, and the rightward facing arrow shows the location of the YOR1 5' mRNA end point. ATG indicates the location of the start of the YOR1 open reading frame. The amount of 5' DNA present in each YOR1-lacZ fusion gene is shown by the length of the line, and the coordinates of the end point are listed on the left.

A YOR1-lacZ construct with a 5' end point 1065 bp upstream of transcription start generated 15 units/A600 of beta -galactosidase activity in a wild-type strain and 5 units/A600 in a Delta pdr1,3 strain as described before (11). Additional 5' truncations had no significant effect on YOR1-lacZ activity until the region between -222 and -129 was removed. The -129 5' deletion pTH42 produced only 6 units/A600 of beta -galactosidase activity in a wild-type strain, and this reduced activity was not further diminished in a Delta pdr1,3 strain. Further reduction in the extent of YOR1 5'-noncoding sequence progressively reduced beta -galactosidase expression.

These data suggest that the DNA region from -222 to -129 contains the major PDRE and is consistent with our previous identification of a candidate PDRE centered at position -215 (11). This candidate YOR1 PDRE is identical in sequence with the site 2 PDRE present in the PDR5 promoter, which we demonstrated to function as a positive regulatory site for PDR5 transcription (15). These data support the view that the PDRE at position -215 is required for Pdr1p/Pdr3p control of YOR1 gene expression and that this element may represent the only PDRE present in the promoter.

YOR1 Promoter Internal Deletions-- Internal deletions in the YOR1 promoter were constructed to identify cis-acting elements that might have been missed in the 5' deletion analysis. Each internal deletion mutant was assayed in both the wild-type and Delta pdr1,pdr3 strains (Fig. 3).


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Fig. 3.   Internal deletion analysis of the YOR1 promoter region. Cells containing both PDR1 and PDR3 (wild-type) or lacking these two structural genes (Delta pdr1,pdr3) were transformed with YOR1-lacZ fusion plasmids. YOR1-dependent beta -galactosidase activity was determined for each transformant, and the values presented are the average of three determinations ± S.D. The symbols are as in Fig. 2.

An unexpected result from this analysis arose when the behavior of an internal deletion mutant lacking the -115 to -50 region of the YOR1 promoter was analyzed. This mutant YOR1-lacZ gene fusion produced 65 units/A600 in wild-type cells, and this elevated activity was only mildly affected by loss of PDR1 and PDR3 (reduced to 42 units/A600). A more extensive internal deletion mutation, lacking -190 to -50, also produced this same enhanced expression phenotype, with 57 units/A600 in wild-type cells and 45 units/A600 in the Delta pdr1,pdr3 strain. Further elimination of YOR1 promoter sequence (-299 to -50) caused a reduction in the expression of the resulting YOR1-lacZ construct but still produced a construct that was significantly more active than the wild-type, especially in the Delta pdr1/pdr3 background. The most extensive internal deletion mutant assayed (-535 to -50) essentially eliminated YOR1 expression.

The finding that deletion of the -115 to -50 region served to elevate expression of YOR1-dependent beta -galactosidase activity was consistent with the belief that loss of this segment of YOR1 enhanced gene transcription. Since the deleted region mapped close to the YOR1 mRNA start site, one possible concern was that the increase in fusion gene expression came about from a change in mRNA form, influencing fusion gene translation rather than amount of YOR1-lacZ mRNA. To evaluate this possibility, we analyzed the 5' ends of the the YOR1-lacZ transcripts produced in these deletion mutants. Each fusion gene was transformed into a strain lacking the 5' end of the endogenous YOR1 locus and the transcription start sites produced by each fusion gene analyzed by RNase mapping (Fig. 4).


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Fig. 4.   RNase mapping of YOR1-lacZ transcripts. Total RNA was isolated from the indicated S. cerevisiae strains. For each sample, the radioactive RNA probe was incubated with RNA, digested with RNase as described by the manufacturer (Ambion), and the protected fragments resolved on 6% urea/polyacrylamide gel electrophoresis. A, RNase protection using a YOR1-specific probe. Lane 1 represents the probe subjected to the RNase digestion protocol in the absence of RNase whereas lane 2 is identical but has RNase added. RNA was isolated from cells containing the wild-type YOR1 allele or the yor1-Delta 1::hisG allele as indicated. The bar of increasing width represents RNase digestions done using either 1 or 5 µg of total RNA. The longer protected products in the samples prepared from strains carrying the lacZ fusion plasmids occur due to fact that the probe contains sequences that correspond to the YOR1-lacZ fusion junction not found in the wild-type YOR1 gene. B, RNase protection using a PDC1-specific probe. 1 µg of total RNA was utilized for RNase mapping in each case.

RNase mapping confirmed that the level of YOR1-lacZ mRNA was increased in each promoter derivative that exhibited elevated beta -galactosidase activity. Two differences were seen between the pattern of 5' mRNA ends produced by the promoter mutants lacking the -115 to -50 region and the wild-type YOR1 promoter. First, the mutant promoters gave rise to a new transcription start site located upstream of the normal +1 start site. Second, a transcription start site (approximately +14) that is used less frequently than the +1 site in the wild-type YOR1 promoter was utilized as the major site for transcription initiation when the -115 to -50 segment was deleted. This analysis suggests that the region between -115 and -50 may play a role both in selection of the transcription start site that is utilized during YOR1 gene transcription and acts to negatively regulate YOR1 mRNA production. It should be noted that we cannot exclude that the appearance of these shorter mRNA species might be the result of degradation of a larger transcript.

These data suggest the presence of a positive regulatory site in the YOR1 upstream region between -535 and -299. This control element was designated UASYOR1. Function of UASYOR1 is negatively regulated by an element located between -115 to -50. To define better the negative regulatory site, two internal deletion mutants were prepared that removed smaller segments of the -115 to -50 interval. Each of these mutant YOR1 promoters (-86 to -50 and -115 to -86) had the effect of elevating expression of the resulting construct by approximately 2-fold relative to the wild-type promoter in both genetic backgrounds. Neither of these internal deletion mutants was able to elevate YOR1-dependent beta -galactosidase activity to the same extent as the derivative lacking the -115 to -50 region, suggesting the presence of multiple negative regulatory elements within this region.

The internal deletion series also provided confirmation for the ability of the PDRE located at position -215 to serve as the major, if not sole, mediator of Pdr1p/Pdr3p regulation. The expression levels of internal deletion mutants that lacked this element were not significantly different when introduced into the wild-type or the Delta pdr1,pdr3 strain. Two internal deletion mutants (+27 to +185 and -115 to +185) contain the PDRE but showed no differential expression when assayed in wild-type and the Delta pdr1,pdr3 strain. This lack of response to PDR1 and/or PDR3 was likely due to the highly defective nature of these derivatives as the expression levels supported by these two mutant promoters represented only 5-13% that of the wild-type YOR1-lacZ fusion, respectively.

Pdr1p Binds to an Element in the YOR1 Promoter-- The results of the 5' and internal deletion analysis of the YOR1 promoter strongly suggested that the PDRE located at -215 was likely to serve as a site for positive control of gene expression by Pdr1p (and likely Pdr3p). To test this idea directly, we produced Pdr1p in bacteria and assayed the ability of this recombinant protein to bind to the YOR1 PDRE. Protein extracts were prepared from E. coli cells carrying the empty expression vector or the same vector expressing an N-terminal fragment of Pdr1p that contains the zinc finger DNA binding domain. This Pdr1p fragment has previously been found to recognize the PDREs in the PDR5 promoter (15). Protein extracts were incubated with radiolabeled DNA templates prepared from the wild-type YOR1 promoter or from a mutant YOR1 promoter that lacks the PDRE. This mutant PDRE was constructed by changing 2 base pairs that have previously been found to be essential for function of a PDRE in the PDR5 promoter (15). The results of this DNase I protection experiment are shown in Fig. 5.


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Fig. 5.   DNase I footprint analysis of Pdr1p binding to the YOR1 PDRE. Binding of bacterially produced Pdr1p to either the wild-type PDRE (wt-PDRE) or a 2-bp substitution mutant (Mut-PDRE) was assessed using a DNase I protection assay. AG and CT indicate the purine- and pyrimidine-specific Maxam/Gilbert chemical sequencing reactions performed on the same restriction fragment used for DNase I protection. Individual DNase I protection assays were carried out in the absence of protein (No Protein), bacterial protein extract from cells carrying the empty expression vector (pOTSNcoI2), or with bacterial protein extract from cells expressing Pdr1p (Pdr1p). The sequence of the sense strand of YOR1 is shown on the left with the DNA sequence protected from DNase I cleavage underlined.

Bacterially expressed Pdr1p was able to protect the YOR1 DNA region from -226 to -208 from DNase I cleavage. No protection was seen if protein was omitted or if an equal amount of protein extract prepared from cells containing the empty expression vector was used. The mutant YOR1 promoter with the 2-base pair substitution mutation in the PDRE was not bound by Pdr1p.

This finding indicates that Pdr1p can bind in vitro to the YOR1 PDRE and that this binding can be blocked by an alteration in the PDRE. To confirm that the PDRE is the in vivo target of Pdr1p action, we analyzed the function of the YOR1 promoter containing the 2-base pair substitution in S. cerevisiae cells.

The PDRE Is Required for Control of YOR1 Expression by Pdr1p-- To assess the contribution of the PDRE to YOR1 promoter function, the mutant PDRE was introduced into the context of the YOR1-lacZ fusion gene present in pSM109-4. Both the wild-type and mutant PDRE-containing YOR1-lacZ fusion genes were transformed into Delta pdr1,pdr3 cells along with a low copy plasmid vector or this same vector plasmid carrying wild-type PDR1 or the dominant PDR1-6 allele. YOR1-dependent beta -galactosidase activities were then determined for each transformant (Fig. 6).


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Fig. 6.   YOR1 PDRE is required for response to PDR1. A cell lacking both PDR1 and PDR3 was transformed with a YOR1-lacZ fusion plasmid either containing the wild-type YOR1 promoter sequence (wild-type) or carrying the 2-bp substitution mutation in the PDRE (mutant PDRE). Each fusion plasmid was co-transformed with a low copy plasmid vector (pRS315), this vector carrying wild-type PDR1 (PDR1) or the low copy plasmid containing a gain-of-function PDR1 allele (PDR1-6). YOR1-dependent beta -galactosidase activities were determined for each transformant. The values presented are the average of at least three independent determinations.

The YOR1-lacZ fusion gene exhibited a strong increase in beta -galactosidase levels in the presence of the PDR1-6 allele. The 2-base pair mutation in the PDRE completely blocked the ability of the fusion gene to respond to the PDR1-6 allele. The mutant PDRE also reduced the ability of the presence of the wild-type PDR1 gene to enhance YOR1-lacZ expression. The levels of YOR1-dependent beta -galactosidase activity were similar, irrespective of the presence of the PDRE in the Delta pdr1,pdr3 cells carrying the low copy vector.

These data provide a strong argument that the PDRE located at position -215 is the only site for Pdr1p (and likely Pdr3p) control of YOR1 transcription. We next evaluated the phenotypic requirement for the PDRE in YOR1-mediated oligomycin resistance.

Normal Oligomycin Tolerance Requires the Presence of a Functional YOR1 PDRE-- The 2-base pair substitution mutation in the PDRE of a YOR1-lacZ fusion gene blocked the ability of this promoter to respond to PDR1. To evaluate the consequence of loss of this sequence element on YOR1-mediated oligomycin tolerance, the mutant PDRE was introduced into the context of the wild-type YOR1 structural gene. This altered YOR1 structural gene was introduced into yor1 cells along with a low copy plasmid carrying the PDR1-6 allele or the vector plasmid alone. An equivalent wild-type YOR1 gene was transformed in a parallel fashion to provide a control for normal YOR1 function. Appropriate transformants were placed on rich medium lacking or containing oligomycin (Fig. 7).


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Fig. 7.   Normal oligomycin resistance mediated by YOR1 requires the presence of an intact PDRE. A strain lacking the chromosomal YOR1 gene was transformed with a low copy plasmid carrying the wild-type YOR1 locus (Wild-type PDRE) or a YOR1 gene containing the 2-bp substitution mutation in the PDRE (Mutant PDRE). These plasmids were co-transformed with a low copy vector plasmid (pRS315) or the same vector plasmid carrying a gain-of-function PDR1 allele (PDR1-6). Appropriate transformants were grown to an A600 of 1 and 1000 cells spotted on medium lacking drug (YPGE) or containing the indicated concentration of oligomycin (0.5 µg/ml oli).

Loss of the PDRE in the YOR1 promoter eliminated the ability of this mutant gene to normally complement the oligomycin hypersensitivity of the yor1 cells. This lack of complementation could not be suppressed by the presence of the PDR1-6 allele. The wild-type YOR1 gene conferred stronger oligomycin resistance in response to the PDR1-6 allele than in the presence of the wild-type PDR1 gene, consistent with the behavior of the YOR1-lacZ gene (Fig. 6). These data confirm that the PDRE is both required for normal Pdr1p responsiveness of the YOR1 promoter as well as for wild-type oligomycin tolerance.

Evidence for Promoter-specific Function of the Pdr1p-independent YOR1 UAS-- Along with the PDRE, the analysis described above pointed to the presence of a second positive transcriptional control element located between -299 and -535 (UASYOR1). To explore the function of this positive regulatory region, we constructed promoter fusions between various upstream fragments of YOR1 and two different UAS-deficient promoter-lacZ fusion constructs. The two promoter-lacZ constructs were the well characterized CYC1-lacZ fusion gene (22) or a similarly deleted PDR5-lacZ fusion that we have previously shown to lack its UAS (10). The YOR1 promoter fragments were placed upstream of these test promoters and introduced into Delta pdr1,pdr3 cells along with a low copy plasmid carrying the wild-type or PDR1-6 alleles of PDR1 or the vector alone. YOR1 promoter fragments were inserted upstream of the heterologous promoters in the same relative orientation as in the native YOR1 promoter. beta -Galactosidase activities of appropriate transformants were then determined (Table I).

                              
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Table I
Upstream activation sequence function of YOR1 5'-noncoding DNA fragments
A strain lacking both PDR1 and PDR3 was transformed with low copy plasmids carrying the wild-type PDR1 gene (PDR1) or the mutant PDR1-6 allele (PDR1-6). The low copy vector (pRS315) was introduced as a control for the absence of Pdr1p and Pdr3p. Three different lacZ fusion gene constructs were also introduced into each of the different PDR1 backgrounds. These plasmids were low copy lacZ fusions to either PDR5 or CYC1 promoters that lacked all wild-type UAS information. Two different YOR1 5'-flanking DNA elements were inserted upstream of the PDR5-lacZ and CYC1-lacZ gene fusions, corresponding to the region from -1065 to -115 or -1065 to -190. Transformants were grown in SD medium with necessary supplements and beta -galactosidase activities determined as described (22).

The successful use of the disabled CYC1-lacZ fusion plasmid as a probe for UAS function has been well documented (22), and these were the first YOR1 promoter fusions that were analyzed. None of the YOR1 upstream fragments tested were able to significantly activate CYC1-dependent lacZ expression, even in the presence of the gain-of-function PDR1-6 allele. To examine the possibility that the lack of YOR1 upstream fragment function might reflect the need for a different promoter context, we employed a fusion gene corresponding to a locus that, like YOR1, is regulated by Pdr1p/Pdr3p. PDR5 is responsive to Pdr1p/Pdr3p and contains multiple PDREs (9, 10, 15, 30). A deleted version of a PDR5-lacZ fusion plasmid carrying 112 bp upstream of the transcription start site was used as a host for the same YOR1 promoter fragments that did not function when placed upstream of CYC1. We have previously shown that the PDR5-lacZ plasmid (pPBDelta 112) lacks all normal PDR5 UAS function (10). Each of the YOR1-PDR5-lacZ fusion genes was transformed into Delta pdr1,pdr3 cells along with a low copy vector plasmid or the same vector carrying wild-type or the PDR1-6 allele of PDR1.

YOR1 promoter fragments extending from -1065 to -115 or -1065 to -190 were able to stimulate PDR5-lacZ expression in all genetic backgrounds tested, unlike similar derivatives placed upstream of CYC1-lacZ. Both of these YOR1 DNA regions contained the PDRE and were highly responsive to the presence of PDR1-6. The finding that these YOR1 fragments are able to activate expression of the PDR5-lacZ fusion gene in a Delta pdr1,pdr3 background confirms that a Pdr1p/Pdr3p-independent regulatory region is present in each of these YOR1 promoter segments. Additionally, the increase in transactivation seen when either of these two YOR1 fragments are placed upstream of the truncated PDR5 promoter but not CYC1 indicates that the PDR5 promoter context is permissive for function of the YOR1 upstream fragments, whereas the CYC1 context is not. In other experiments, we have found that a UASYOR1-containing fragment lacking the YOR1 PDRE is still able to activate function of the PDR5-lacZ fusion gene, albeit weakly.2 Possible explanations for the observed difference in YOR1 promoter fragment function between YOR1-CYC1 and YOR1-PDR5 chimeras will be considered below.

    DISCUSSION
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Although both PDR5 and YOR1 lie under the umbrella of PDR1/PDR3 gene regulation, these two loci do not exhibit common promoter structures. Both PDR5 and YOR1 contain PDREs, with PDR5 having three elements and YOR1 only one. The sequence of the YOR1 PDRE is identical to PDR5 PDRE site 2 (15). PDR5 expression is strictly dependent on the presence of either PDR1 or PDR3, whereas YOR1 expression exhibits a large component that is PDR1/PDR3-independent. This finding suggested the presence of other positive regulatory elements for transcription in the YOR1 promoter, a suggestion we have confirmed in the current study.

The second positive regulatory element for YOR1 expression (UASYOR1) lies between positions -535 and -299. The function of this element was not detected by 5' deletion analysis as UASYOR1 activity is under negative control by an upstream repression sequence (URSYOR1) lying between -115 and -50. YOR1 appears to represent a new example of a PDR gene in terms of having this fairly complex arrangement of interacting positive and negative transcriptional control elements superimposed on the PDRE-mediated control of expression. Studies of the PDR5 promoter have not indicated the presence of any regulatory sequences other than the PDREs (10, 15). Removal of the PDREs from the PDR3 promoter eliminates the ability of this gene to elevate the cycloheximide resistance of a Delta pdr1,pdr3 cell (14). This comparison suggests that YOR1 is likely to integrate transcriptional control signals from factors other than Pdr1p/Pdr3p. Identification of these other factors will provide new insight into the physiological role of Yor1p.

Another unique feature presented by the YOR1 promoter is the presence of a single PDRE in its 5'-noncoding region. All other known PDRE-containing promoters contain at least 2 (PDR3) to 3 (PDR5) PDREs. Interestingly, these multiple PDRE-containing promoters also exhibit markedly more responsiveness to Pdr1p/Pdr3p than does YOR1. This observation is consistent with our previous finding that each PDRE in the PDR5 promoter contributes approximately equally to the overall response of PDR5 to Pdr1p levels (15). These data suggest that the degree of Pdr1p/Pdr3p responsiveness of a given promoter can be largely predicted by the number of PDREs found in the promoter of interest.

The presence of the negatively acting URSYOR1 element was detected by an increase in YOR1-lacZ expression that occurred upon deletion of the -115 to -50 region of the YOR1 promoter. We believe that this increase in expression is due to an unmasking of the UASYOR1 rather than a change in spacing of promoter elements for two reasons. First, several other YOR1 internal deletions also exhibit this enhancement of expression (Fig. 3) while each varies significantly in terms of its position relative to the transcription start site. Second, when these same internal deletion end points are used to construct chimeric promoters with PDR5, all are capable of conferring significant Pdr1p/Pdr3p-independent levels of lacZ expression (Table I). The simplest explanation of these results is that the -535/-299 UASYOR1 is activated upon removal of the URSYOR1 site. Additionally, the -115 to -50 promoter segment appears to contribute to 5' end production in YOR1 transcription.

Other S. cerevisiae promoters have been found to have this arrangement of positive elements controlled by linked negative regulatory sites. The CAR1 gene encodes arginase and is highly repressed unless arginine is present as the nitrogen source (31). CAR1 also has a UAS element that is under control of a downstream URS. The negative regulation of this downstream URS is overcome in CAR1 when the inducer of CAR1 expression is present. Interestingly, this URS does not respond to the presence of inducer when it is placed between the UAS and TATA region of the heterologous CYC1 gene (32). The inability of this CAR1 promoter segment to function in a heterologous environment is reminiscent of the activity we have seen for the YOR1 promoter fragments placed upstream of CYC1 or PDR5. We do not yet know if the YOR1-PDR5 chimeras are active due to the presence of an additional positive site or the absence of a negatively acting element that is found in CYC1.

The behavior of the YOR1 UAS region placed upstream of CYC1-lacZ was unexpectedly complex. This UAS region was not able to stimulate CYC1-dependent beta -galactosidase activity to an appreciable extent, irrespective of the genetic background examined. Even in the presence of the PDR1-6 allele, the YOR1-CYC1-lacZ fusion genes were essentially inactive. This was especially surprising considering that we previously have shown that an oligonucleotide corresponding to a PDRE present in the PDR5 promoter is capable of acting as a UAS when placed upstream of the same CYC1-lacZ test promoter (15). There are two important differences between the current study and these previous experiments. First, a different PDRE was used in each experiment. Second, the YOR1 PDRE was provided in the context of its wild-type upstream sequence while the PDR5 PDRE was inserted as an oligonucleotide. These differences are likely to explain the distinctions in the behavior of these PDREs as UAS elements for CYC1.

One of the major differences between the PDR5 and CYC1 fusion vectors used to test YOR1 UAS function is likely to be the TATA element provided by each fusion plasmid. The inability of the YOR1 UAS to stimulate CYC1-lacZ expression might be explained by an inability of this UAS region to productively function with the TATA elements contributed by CYC1. Differential TATA utilization has been seen for the HIS3 (33) and HIS4 (34) promoters, consistent with the idea that certain TATA elements are only capable of responding to particular types of transcriptional regulatory proteins. Alternatively, the presence of some feature of the CYC1 promoter may act to inhibit the productive interaction between the YOR1 fragments and the CYC1 TATA region. Further experimentation is required to discriminate between these possible explanations for the unusual promoter specificity of the YOR1 UAS.

Recent experiments have demonstrated that YOR1 is required for resistance to the drug reveromycin A and that YOR1 transcription was inducible by this drug (35). It will be of great interest to determine which DNA sequences are involved in this observed transcriptional induction of YOR1. The work presented here provides important information about candidate regulatory elements that may mediate reveromycin A induction of YOR1 expression. Future work will determine if this drug inducibility of YOR1 acts through the PDRE or the other Pdr1p/Pdr3p-independent transcriptional control elements we have identified.

    ACKNOWLEDGEMENTS

We thank Laurence Lambert for constructing and providing the Myc-Pdr1p expression plasmid and David Katzmann for a critical review of the manuscript.

    FOOTNOTES

* This work was supported by National Institutes of Health Grant GM49825 (to W. S. M.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Established Investigator of the American Heart Association. To whom correspondence should be addressed. Tel.: 319-335-7874; Fax: 319-335-7330; E-mail: moyerowl{at}blue.weeg.uiowa.edu.

1 The abbreviations used are: ABC, ATP binding cassette; UAS, upstream activation sequence; bp, base pair; PDRE, Pdr1p/Pdr3p response element; PCR, polymerase chain reaction.

2 T. Hallstrom, unpublished data.

    REFERENCES
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

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