A Rare 920-Kilobase Chromosomal Inversion Mediated by IS1 Transposition Causes Constitutive Expression of the yiaK-S Operon for Carbohydrate Utilization in Escherichia coli*

Josefa Badía, Ester IbáñezDagger , Montserrat Sabaté, Laura Baldomà, and Juan Aguilar§

From the Department of Biochemistry, School of Pharmacy, University of Barcelona, Diagonal 643, 08028 Barcelona, Spain

    ABSTRACT
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

The regulator of the yiaK-S operon, currently assigned a carbohydrate utilization function in Escherichia coli, is inactivated by a genome rearrangement that leads to the constitutive expression of the operon. The yiaK-S constitutive cells acquire the ability to utilize the rare pentose L-lyxose. Restriction analysis and sequencing of the regulator gene indicate that it is disrupted by foreign DNA. The insert consists of a large inverted fragment of DNA of 920 kilobases flanked by two IS1 elements with opposite polarity. One corresponds to that found naturally at min 0.4 of the bacterial chromosome and the other to a new copy transposed into the regulator gene located at min 80.6. This insertion-inversion could be the result of the intramolecular transposition mechanism itself, a gene rearrangement rarely originated by IS1. Alternatively, it could be attributed to the homologous recombination between the IS1 at min 0.4 and the IS1 transposed intermolecularly into the yiaK-S regulator gene. The participation of a rare IS1-mediated inversion in the evolution of a stable phenotype is thus identified.

    INTRODUCTION
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Most known mobile IS1 can be inserted at a large number of chromosomal sites. They can subsequently be recombined and excised precisely or imprecisely, and they can engender inversions or deletions in neighboring chromosomal regions (1, 2). The presence of these insertion elements in the chromosome of Escherichia coli and other bacteria such as Salmonella typhimurium or Bacillus subtilis originally became apparent as a result of the transposition of these sequences from their natural positions into functional genes, which can lead to recognizable mutant phenotypes. As a corollary, their derived deletions, inversions, and other gene rearrangements could also result in phenotypic changes that could normally be stable and easily identifiable.

Relevant models of cryptic gene activation by insertion of IS elements into the regulator or promoter region have been reported by several authors. The cryptic cel operon is activated in E. coli by insertion of IS1, IS2 or IS5 into a region 72-180 bp upstream from the transcription start site due to an unknown mechanism that does not involve provision of a promoter sequence (3). It has also been described that certain excisions of inserted elements function as activation mechanisms. For example, phnE is activated by excision of an 8-bp insertion, which causes a frameshift mutation present in the cryptic phn operon (4). In another context, transpositions and subsequent deletions or inversions cause changes in the regular expression of several gene systems. Among the mutations mapped in the promoter region of the araBAD operon (5) two insertions of IS1 were found, which resulted in reduced expression of this operon and hence the inability to utilize arabinose (6). Inversion, of uncertain origin in this case, of a 6-kb fragment containing slpA and slpB in Lactobacillus acidophilus, leads to the positioning of the silent slpB gene behind the slpA promoter, which is located outside the inverted segment, whereas the slpA gene is stored at the silent position of the slpB gene (7). Various chromosomal rearrangements subsequent to Tn10-mediated inversions that connect the udp gene and promoters of rrn operons were characterized in E. coli (8), some of them involving more complicated events such as partial internal reinversions. Overexpression of udp in these mutants complemented thymine auxotrophy. Another example of these events is a rearrangement subsequent to a REP-mediated gene duplication, which placed the his operon under the control of argR in Salmonella typhimurium (9, 10).

Unexplained inversions and chromosomal rearrangements of up to one-third of the total length have been found in E. coli (11, 12) or Neisseria gonorrhoeae (13). Although precise phenotypic changes were not determined in these models, the effects of these major chromosomal rearrangements on cell fitness have been studied by Hill and Gray (14) following the growth of two E. coli strains with inversions between rRNA operons. According to this and other studies (15) establishment of the inversions appears to depend on the type of genes present within the chromosomal interval.

In thinking about evolutionary mechanisms, rearrangements have not been given the consideration that point mutations have received (16). Nevertheless, rearrangements subsequent to insertion element-jumping events appear to play a role in evolution. Among the insertion sequences able to participate in genome rearrangements, IS1 is one of the smallest autonomous transposable elements known. It can transpose in both a replicative and a conservative manner, but it rarely gives rise to inversions (17). An inversion induced by a resident IS1 of the lactose transposon Tn951 (18) may be mentioned as one of the few instances of IS1-mediated inversions documented so far. Here the yiaK-S operon (locus lyx at 80.6 min of the E. coli chromosome) (19), has been found to be constitutively expressed in the mutant strain JA134 selected for its ability to grow on the rare pentose L-lyxose (20). The constitutive expression of the operon has been shown to be the result of a chromosomal inversion secondary to an IS1 transposition into the repressor gene of the yiaK-S operon. The metabolic consequences of yiaK-S repressor disruption were found to be stable.

    MATERIALS AND METHODS
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Bacterial Strains and Cell Growth-- The bacterial strains used in this work are E. coli K12 derivatives. ECL1 is HfrC phoA8 relA1 tonA22 T2r (lambda ) (21). JA134 is Lyx+ rhaB but is otherwise isogenic to ECL1 (20). JA161 obtained from ECL1 in this work is yiaJ::cat. NM539 is F-, supF, hsd (rk-, mk+), lacY (P2) (22), and JC7623 is arg thi thr leu pro his strA recB21 recC22 sbcB15 (23).

Cells were grown aerobically on Luria broth or minimal medium (24). For growth on minimal medium, carbon sources were added at concentrations of 12 mM for L-lyxose, 20 mM for glycerol, and 0.5% for casein acid hydrolysate. When necessary, the antibiotics were used at the following concentrations: ampicillin, 100 µg/ml; tetracycline, 12.5 µg/ml; chloramphenicol, 30 µg/ml. 5-Bromo-4-chloro-3-indolyl-beta -D-galactoside and isopropyl-beta -D-thiogalactoside were used at 30 and 10 µg/ml, respectively.

Preparation of Cell Extracts and Enzyme Activities-- Cell extracts were prepared as described previously (24). L-Xylulose kinase activity was determined from the rate of NADH oxidation. The reaction mixture (1 ml) contained 100 mM Tris-HCl buffer (pH 8.0), 4 mM L-xylulose, 1 mM ATP, 1.75 mM phosphoenolpyruvate, 3.5 mM MgCl2, 0.25 mM NADH, 0.35 mM reduced glutathione and 2 units/ml of pyruvate kinase and lactate dehydrogenase.

DNA Manipulation-- Chromosomal DNA was obtained using the genomic DNA purification kit (Promega, Madison, WI). Plasmid DNA was routinely prepared by the boiling method (25). For a large scale preparation, a crude DNA sample was purified through a column (Qiagen GmbH, Düsseldorf, Germany). Other standard DNA manipulations were performed essentially as described by Sambrook et al. (26).

The DNA sequence was determined by the dideoxy chain termination procedure of Sanger et al. (27) by using the T7 sequencing kit from Amersham Pharmacia Biotech. Double-stranded plasmid DNA was used as the template. Ordered deletions were obtained with the Erase-a-Base system (Promega). Universal or T3 primers were used. To avoid sequencing errors, both strands were sequenced by regular dGTP as well as dITP or 7-deaza-dGTP reactions. Primer A (TTTGGTGGTCAGGCGAT) and primer B (CACACTTTGCGCTACCA) were also used to confirm the sequence of the two insertional points in the regulator gene. The GenBankTM data base was searched with the TFASTA algorithm (28).

For Southern blot analysis, restriction fragments were separated by agarose gel electrophoresis, blotted onto nylon filters, and fixed by incubating at 80 °C for 2 h. A 32P-labeled probe was used in the detection of hybridization products.

Library Construction-- Chromosomal DNA from strains ECL1 and JA134 were totally digested with SalI and then ligated with the lambda  EMBL4 cleaved with SalI as described by Sambrook et al. (26). The packaging of ligation mixtures in vitro was performed by using the Packagene extract system (Promega) and propagated on NM539 (P2) for Spi selection of recombinant phages. For strain JA134 a partial Sau3A genomic library was also prepared in a BamHI digested EMBL4 phage.

Isolation of RNA and Northern Blot Hybridization-- For preparation of total RNA, cells of a 25-ml culture grown to an A650 of 0.5 were collected by centrifugation at 5,000 × g for 10 min and processed as described by Belasco et al. (29). Northern blot hybridization was performed with each RNA sample (10 µg) by following the procedure described previously by Moralejo et al. (30).

Inactivation by CAT Insertion-- The chloramphenicol resistance cassette CAT19 was used in the gene inactivation experiments (31) by inserting it into the restriction site indicated below. This cassette had no terminator in the downstream region of the CAT gene and thus did not cause polarity when inserted in the same orientation as the interrupted gene. The plasmid-carrying inactivated gene was linearized by SalI digestion and used to transform strain JC7623 to chloramphenicol resistance. This strain efficiently recombines linear DNA into its chromosome (32). P1vir lysates obtained from the selected chloramphenicol resistance recombinants were used to transduce the CAT insertions into the parental strain ECL1 or the mutant strain JA134.

PCR-- Reactions were performed on purified chromosomal DNA with Vent DNA polymerase (New England Biolabs, Beverly, MA) according to the manufacturer's protocol and 100 pmol of each of the oligonucleotide primers in 100 µl of reaction mixture. The reaction routinely consisted of 30 cycles. The primers used to amplify the junction region proximal to the origin (min 0) in the mutant strain were: REG2 (GTCACTTTCATCCCAGGC) and HIS1 (GAAATCATCACCTACGGC) and those used to amplify the distal junction region were REG1 (CGCCATGTAGATCTTGC) and 28K1 (AGCCTGGCCTTTGGTAGC). For Southern analysis covering the full-length of inverted DNA, over 50 probes of 1 kb at intervals of approximately 15 kb were prepared by PCR using pairs of primers of 18-20 bp (not shown), which were designed following the E. coli genome sequence in GenBankTM. PCR products were resolved by 2% agarose gel electrophoresis and purified by QIAquick PCR purification kit (Qiagen GmbH).

    RESULTS
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Expression of yiaK-S Operon-- Mutant strain JA134 is an ECL1 derivative selected for its ability to grow on the rare sugar L-lyxose, which does not support growth of wild-type strain. This mutant was characterized by constitutively expressing the L-xylulose kinase activity involved in the L-lyxose metabolism (20). The gene encoding L-xylulose kinase was cloned in plasmid pJC2 (Fig. 1). Here we have attempted the sequencing of the insert present in pJC2. This sequence displayed total identity with that of the open reading frame located between positions 6123 and 7619 in the entry AE000435 (section 325 of 400). The L-xylulose kinase gene was thus identified as part of a gene cluster, which according to its location at 80.6 min of the E. coli genome has been designated as yiaJ-S (33). Based on the genome analysis by Sofia et al. (34) this cluster is formed by nine genes encoding products for carbohydrate metabolism (yiaK to yiaS), transcribed clockwise, and one (yiaJ) transcribed anticlockwise (Fig. 1).


View larger version (12K):
[in this window]
[in a new window]
 
Fig. 1.   Physical and genetic map of the region encompassing the yiaK-S operon. The bar represents the SalI genome fragment with the relevant restriction sites labeled as follows: A, AgeI; B, BamHI; Bg, BglII; Bs, BstXI; H, HindIII; P, PstI; S, SalI; V, EcoRV; and X, XhoI. Arrows indicate the extent and direction of transcription of the genes included in the yiaK-S operon. Lines show the inserts of the indicated plasmids. CAT corresponds to a cassette-encoding chloramphenicol acetyltransferase used for site-directed mutagenesis of yiaJ.

Northern analysis of total RNA of strain JA134 using probes of the structural genes yiaP and yiaR showed that transcription of the operon was detected in all growth conditions used. Fig. 2A displays a typical experiment with a yiaP internal probe. The probe was 32P-labeled by the random primed method (26) using a 360-bp AgeI-XhoI fragment as template. The pattern of transcript molecules always showed several bands due to fragmentation of the long messenger RNA. In contrast, no transcript was found in wild-type strain preparations run in parallel. Determination of L-xylulose kinase in crude extracts of the same cultures used for isolation of RNA displayed similar high activity values of 105-115 nmol/min/mg. These results allowed us to use L-xylulose kinase as a reporter activity of the expression of the yiaK-S operon.


View larger version (23K):
[in this window]
[in a new window]
 
Fig. 2.   Northern blots of total RNA from mutant strain JA134 and wild-type strain ECL1. Cells were grown on casein acid hydrolysate in the absence (-) or in the presence (+) of L-lyxose. Blots were hybridized with 32P-labeled fragments corresponding to the structural gene yiaP (A) or the regulator gene yiaJ (B). Size of the transcripts in kb according to markers used in the experiment are indicated.

Identification and Inactivation of the Regulator Gene-- The putative role of yiaJ gene as regulator of yiaK-S was approached by characterization and inactivation of this gene. Sequence analysis of the regulator gene product and comparison with other regulator sequences showed a helix-turn-helix motif of binding to DNA and a second motif of unknown function (Fig. 3). Both motifs were also found in regulator proteins such as glycerol activator (streptomyces), acetate repressor (S. typhimurium), or Kdg repressor involved in pectinolysis in Erwinia chrysanthemi, all belonging to the known "IclR" family (35).


View larger version (50K):
[in this window]
[in a new window]
 
Fig. 3.   Nucleotide sequence and amino acid translation of the yiaJ gene encoding the repressor of the yiaK-S operon. 5'-proximal shaded region corresponds to the helix-turn-helix motif, whereas the 3'-proximal one corresponds to the IclR family consensus of undetermined function. Target sequence for the IS1 transposition is boxed, and the position where the gene has been interrupted by the insertion in strain JA134 is indicated by the arrow. The BglII restriction site used for site-directed mutagenesis is labeled.

The proposed regulator gene upstream of the operon was inactivated by a CAT cassette insertion in wild-type cells. To this end a clone encompassing the complete operon was isolated from a SalI chromosomal library of wild-type strain ECL1 prepared as indicated under "Materials and Methods." The clone was selected by hybridization with a probe 32P-labeled by the random primed method (26) using as template a 520-bp XhoI-EcoRV internal fragment obtained from pJC2. The 3.2-kb HindIII fragment containing the proposed regulator gene was subcloned in pBR322 (plasmid pJB1). Inactivation of the putative regulator gene was carried out by insertion of CAT cassette in the BglII restriction site present in plasmid pJB1 insert, yielding plasmid pJB2 (Fig. 1) and the mutant gene transferred to the wild-type ECL1 background yielding strain JA161. The correct integration of the CAT resistance gene was confirmed by Southern blot analysis (not shown).

The effects of the inactivation of the putative regulator gene were analyzed in cell extracts of strain JA161 by measuring the L-xylulose kinase activity, which was found to be present in growth conditions that did not yield this same activity in wild-type cells. Furthermore, Northern analysis of total RNA of strain JA161 using probes of the structural genes yiaP and yiaR (not shown) were indistinguishable from those obtained with total RNA of strain JA134. When a BglII-AgeI probe internal to the regulator gene was used, Northern analysis showed a single constitutive transcript in RNA preparations of strain ECL1 (Fig. 2B) and no transcript in the CAT mutated strain JA161 (not shown). The derived constitutivity in the expression of the yiaK-S operon in strain JA161 indicated that the yiaJ had regulatory functions and that this system is under the control of a repressor protein encoded by this gene.

Characterization of the Mutation in the Regulator Gene of Strain JA134 -- The constitutive expression of yiaK-S operon in strain JA134 thus suggested that the mutation responsible could affect this operon regulatory region. To characterize the mutation, a probe 32P-labeled by the random primed method using a 640-bp EcoRV-HindIII fragment belonging to yiaK (see Fig. 1) was used to hybridize Southern blots of the wild type and mutant JA134 DNA digested with SalI, BamHI, or HindIII. The hybridization pattern obtained in these experiments for each of the indicated restriction enzymes was clearly different in the wild type and mutant strain (Fig. 4). These results are consistent with the presence of an insertion or deletion in the nearby region encompassing the regulator and the yiaK-S operon promoter.


View larger version (36K):
[in this window]
[in a new window]
 
Fig. 4.   Southern blot analysis of the yiaJ region. Comparison of Southern blot patterns between genomic DNA of strain ECL1 (lanes 1) and genomic DNA of strain JA134 (lanes 2) digested with SalI, BamHI, or HindIII. Blots were hybridized with a 32P-labeled probe corresponding to yiaK sequences (see text). Fragment size markers in kb are indicated on the right side of the panel.

Furthermore, the search of the regulator gene transcript using a BglII-AgeI probe internal to the regulator gene, showed no band of hybridization (Fig. 2B), consistent with the type of proposed mutation that could abolish initiation of transcription or synthesize an anomalous messenger not detected with the probe used.

Insertion-Inversion into the Repressor-- The absence of transcript of the regulator gene reported above was the result of a mutation in this gene and is not compatible with a mutation in the promoter region of the structural genes. For this reason the nature of the mutation was studied in the yiaJ regulator gene. A clone of the mutated regulator was isolated from a SalI library of strain JA134, using a probe prepared with the internal BglII-AgeI fragment (Fig. 1). Its SalI insert was transferred to Bluescript vector yielding clone pJB3 (Fig. 5). Restriction analysis of the insert in plasmid pJB3 indicated that foreign DNA sequences were present in the regulator.


View larger version (15K):
[in this window]
[in a new window]
 
Fig. 5.   Diagram of the inversion-insertion mutation in yiaJ gene of strain JA134. The map shows the location in the genomic DNA (open bar) of the inverted region (thick line) with a long nonrepresented interval (dashed line). For better comprehension the relevant restriction sites as well as the minute positions are indicated on the DNA. The extent and direction of transcription of different genes and IS1 copies (black arrows) and of the interrupted gene (shaded arrow) are also presented below. In the upper part, the sequence of the junction points is shown with the IS1 sequence boxed and the transposition-generated repeats derived from the target regulator sequences in bold. The bottom part displays the extent of the inserts in the plasmids used in the analysis of the junction regions (thin lines). A, AgeI; B, BamHI; Bg, BglII; H, HindIII; S, SalI; and V, EcoRV.

To further identify the DNA fragment not belonging to the regulator gene, different subclones of plasmid pJB3 were prepared and their terminal ends were sequenced. Computational analysis showed 100% similarity with sequences corresponding to IS186, and to the ant, dnaJ and dnaK genes, all located at 0.4 min. The shortest clone (pJB4) containing the fragment with the joining site for regulator and foreign DNA was obtained and the corresponding insert was totally sequenced. Comparison of the sequences of pJB4 clone with GenBankTM sequences allowed us to locate an IS1 insertion element between sequences corresponding to the regulator gene and those corresponding to the 0.4 min, which were found to be inverted with respect to their natural genome order (see Fig. 5).

To determine the other joining site, not included in pJB3, a clone from a Sau3A library of strain JA134 was isolated using a probe prepared with the yiaK internal fragment EcoRV-HindIII. Likewise, a 2-kb internal HindIII fragment of the Sau3A clone was transferred to Bluescript vector, yielding clone pJB5. Restriction analysis of the insert in plasmid pJB5 indicated again that DNA sequences not belonging to the regulator region were present. Sequencing of clone pJB6, derived from pJB5, showed sequences of the regulator and of another IS1 insertion element determining the other junction point in the interrupted repressor gene. This implied an IS1 transposition into the repressor. This insertion element sits in this case between the 5'-end part sequences of the regulator and sequences identical to those located between positions 20227 and 20460 in the 0-2.4 min region (accession number D10483). The IS1 transposed displayed opposite polarity and a totally identical sequence with the IS1 naturally found in the 0.4 min of the E. coli chromosome (36). The two IS1 elements had the transposition-generated repeats derived from the target regulator sequences on only one side.

The size of the inversion and possible changes in the gene order inside the inverted fragment were checked by Southern analysis. To this end, PCR fragments of about 1 kb were used to prepare 32P-labeled probes every 15 kb along the putative inverted region. Comparison of the patterns in the Southern experiments using digested DNA from wild-type strain ECL1 and mutant strain JA134 with BamHI, EcoRI, SalI, PstI or HindIII restriction enzymes displayed no differences. It was thus established that the inversion was up to 920 kb in length and that no other internal rearrangement occurred in the inverted fragment.

To confirm the specific mutation two oligomers flanking each of the junction sites of the inversion (see "Materials and Methods") were used in amplification of genomic DNA obtained from wild type (strain ECL1) and mutant strain JA134. A PCR product of amplification was found when mutant DNA, but not wild-type DNA, was the template.

Stability of Newly Acquired Phenotype-- The constitutivity of the yiaK-S operon expression subsequent to the IS1 transposition into its regulator implied phenotypic consequences reflected by the acquisition of the ability to utilize L-lyxose. The stability of this mutation is of obvious interest for its evolutionary significance. To determine the stability of the constitutive expression of yiaK-S operon in strain JA134, the permanence of the L-lyxose utilization as a reporter phenotype was followed in its offspring. To this end, a culture of 1 colony-forming unit/ml of strain JA134 was grown on LB broth up to 5 × 109 colony-forming unit/ml. This culture was diluted and reinoculated for a second growth to the same cell densities. The experiment allowed more than 30 generations without any selective pressure for L-lyxose utilization. At the end of the culturing, 5 × 103 colony-forming units were plated on glycerol and replica-plated on L-lyxose. All colonies kept the L-lyxose positive phenotype.

    DISCUSSION
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Among the multiple changes that may originate phenotypic modifications, we present a case in which the regulator gene of an operon is interrupted by an inserted DNA fragment. This fragment, of an unusual length of approximately 920 kb, is flanked by two IS1 elements. Fine analysis of the sequences of plasmids pJB4 and pJB6 suggested that the insertion into the regulator was the result of an intramolecular transposition, leaving the IS1 elements with opposite polarity. Furthermore, an inverted gene order of the foreign DNA is seen in plasmid pJB3, whereas sequences of the repressor and 0.4 min region are found adjacent to each of the terminal ends of the IS1 in plasmid pJB5. Attending to these observations, it is concluded that a genome inversion from 0.4 to 80.6 min, interrupting the regulator gene (yiaJ), has taken place in mutant strain JA134. The indicated region could be inverted in the process of IS1 intramolecular transposition itself as a consequence of ligation in opposite polarity of IS1 ends with the staggered break ends in the target (yiaJ) gene, precisely located in 80.6 min (Fig. 6). However, as indicated by Turlan and Chandler (17) for IS1 and by Weinert et al. (37) for IS903 these insertion-inversion events, although possible in intramolecular transposition, are very infrequent if present at all. The mutation presented here, despite the scarce occurrence of this type of rearrangements, indicates its potential participation in genome evolution. At present we have no information on the frequency of this or similar mutations.


View larger version (17K):
[in this window]
[in a new window]
 
Fig. 6.   Mechanism of the intramolecular transposition-mediated inversion. The model shows how intramolecular transposition of IS1 gives rise to an inversion. Both DNA strands are represented (continuous and dashed lines). A, strand cleavage of the target sequence (dots) in the yiaJ gene (gray bar) and of IS1 (black bar) is indicated by the black arrows. IS1 sense of transcription is indicated by the white-headed arrow, and several gene markers are also indicated. B, transposition of the IS1 into the target sequence in opposite polarity results in a branched molecule at each of the IS1 elements. C, map of the inverted region in mutant strain JA134. Replication (open symbols) through the transposon resolves the branched structure into a linear molecule with an inversion between the two IS1 elements.

Despite the relatively well known genetic organization of IS1, its mechanism of transposition and consequently the resulting rearrangement products are poorly understood. As has been widely discussed (1, 37) another speculative mechanism cannot be ruled out as an alternative to the process described above. Transposition, intermolecular in this case, and subsequent recombination between IS1 at min 0.4 and the IS1 newly inserted into the repressor, could also promote one such rearrangement. Since the products of any of the two mechanisms are indistinguishable, including the distribution of the transposition-generated repeats of the neighbor target sequences, we cannot at present discriminate between them. Nevertheless, the requirement of two independent events in this second proposed mechanism argues against the probability of its occurrence in the selection process.

It has been pointed out by several authors that insertion-inversion events that change the location of certain genes with respect to the origin of replication may be detrimental or lethal (38). This is seen as one of the functional barriers to inversion detection. To explain this, several functional consequences are invoked. They are, among others, changes in copy number of some fundamental genes, obstructive orientation impeding replication, or disturbance of the symmetrical positioning of the origin, requiring one of the growing forks to copy more than half of the chromosome. The fragment inverted in mutant strain JA134 spanned from 80.6 to 0.4 min and thus involved the replication origin oriC located at 84.5 min. It is of interest that in our study, not only was the insertion-inversion mutant viable and able to be isolated, but also growth on different carbon sources and conditions were indistinguishable from that obtained with wild-type strain.

In this model a genome rearrangement (insertion-inversion) of specific phenotypic consequences is presented as well as the mechanism leading to it. This is in contrast to most of the similar or larger insertion-inversions of unknown origin and undetermined phenotypic consequences. The constitutivity of yiaK-S operon, as indicated by the ability to utilize L-lyxose, is a stable genetic trait indicating that the mutation inactivating the repressor does not easily revert. In this context it is of interest to consider that reversion of insertion mutations such as the one described here are likely to be imperfect, leaving the IS1 element or other parts of the transposed DNA in place. These reversions would maintain the target gene interruption, giving rise to stable phenotypes.

Acknowledgments-- We thank Josep Casadesús for critical reading of this manuscript and Robin Rycroft for editorial assistance.

    FOOTNOTES

* This research was supported by Grant PB94-0829 from the Dirección General de Investigación Científica y Técnica, Madrid, Spain, and by the help of the Comissionat per Universitats i recerca de la Generalitat de Catalunya.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) U00039, AJ223474, AJ223475.

Dagger Recipient of a predoctoral fellowship (FPI) from the Comissio Interdepartamental de Recerca i Innovació Tecnològica (Generalitat de Catalunya).

§ To whom correspondence should be addressed. Tel.: 34-3-402-4521; Fax: 34-3-402-1896; E-mail: Jaguilar{at}farmacia.far.ub.es.

1 The abbreviations used are: IS, insertion sequence; CAT, chloramphenicol acetyltransferase; PCR, polymerase chain reaction; bp, base pair(s); kb, kilobase(s).

    REFERENCES
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

  1. Galas, D. J., and Chandler, M. (1989) in Mobile DNA (Berg, D. E., and Howe, M. M., eds), pp. 109-162, American Society for Microbiology, Washington, DC
  2. Kleckner, N. (1990) Annu. Rev. Cell Biol. 6, 297-327[CrossRef]
  3. Parker, L. L., and Hall, B. G. (1990) Genetics 124, 473-482[Abstract/Free Full Text]
  4. Makino, K., Kim, S. K., Shinagawa, H., Amemura, M., and Nakata, A. (1991) J. Bacteriol. 173, 2665-2672[Medline] [Order article via Infotrieve]
  5. Eleuterio, M., Griffin, B., and Sheppard, D. E. (1972) J. Bacteriol. 111, 383-391[Medline] [Order article via Infotrieve]
  6. Miyada, G. C., Sheppard, D. E., and Wilcox, G. (1983) J. Bacteriol. 156, 765-772[Medline] [Order article via Infotrieve]
  7. Boot, H. J., Kolen, C. P. A. M., and Pouwels, P. H. (1996) Mol. Microbiol. 21, 799-809[Medline] [Order article via Infotrieve]
  8. Fonstein, M., Nikolskaya, T., Zaporojets, D., Nikolsky, Y., Kulakauskas, S., and Mironov, A. (1994) J. Bacteriol. 176, 2265-2271[Abstract]
  9. Anderson, R. P., and Roth, J. R. (1978) J. Mol. Biol. 119, 147-166[Medline] [Order article via Infotrieve]
  10. Shyamala, V., Schneider, E., and Ames, G. F.-L. (1990) EMBO J. 9, 939-946[Abstract]
  11. Xia, X. M., and Enomoto, M. (1986) Mol. Gen. Genet. 205, 376-379[Medline] [Order article via Infotrieve]
  12. Hill, C. W., and Harnish, B. W. (1981) Proc. Natl. Acad. Sci. U. S. A. 78, 7069-7072[Abstract]
  13. Gibbs, C. P., and Meyer, T. F. (1996) FEMS Microbiol. Lett. 145, 173-179[CrossRef][Medline] [Order article via Infotrieve]
  14. Hill, C. W., and Gray, J. A. (1988) Genetics 119, 771-778[Abstract/Free Full Text]
  15. Mahan, M. J., and Roth, J. R. (1991) Genetics 129, 1021-1032[Abstract/Free Full Text]
  16. Roth, J. R., Benson, N., Galitski, T., Haack, K., Lawrence, J. G., and Miesel, L. (1996) in Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology (Neidhardt, F. C., Ingraham, J. L., Low, K. B., Magasanik, B., Schaechter, M., and Umbarger, H. E., eds), pp. 2256-2276, American Society for Microbiology, Washington, DC
  17. Turlan, C., and Chandler, M. (1995) EMBO J. 14, 5410-5421[Abstract]
  18. Cornelis, G., and Saedler, H. (1980) Mol. Gen. Genet. 178, 367-374[Medline] [Order article via Infotrieve]
  19. Blattner, F. R., Plunkett, G., III, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, A., Goeden, M. A., Rose, D. J., Mau, B., and Shao, Y. (1997) Science 277, 1453-1462[Abstract/Free Full Text]
  20. Sánchez, J. C., Giménez, R., Schneider, A., Fessner, W. D., Baldomà, L., Aguilar, J., and Badía, J. (1994) J. Biol. Chem. 269, 29665-29669[Abstract/Free Full Text]
  21. Lin, E. C. C. (1976) Annu. Rev. Microbiol. 30, 535-578[CrossRef][Medline] [Order article via Infotrieve]
  22. Frischauf, A. M., Lehrach, H., Poustka, A., and Murray, N. (1983) J. Mol. Biol. 170, 827-842[Medline] [Order article via Infotrieve]
  23. Wackernagel, W. (1973) Biochem. Biophys. Res. Commun. 51, 306-311[Medline] [Order article via Infotrieve]
  24. Boronat, A., and Aguilar, J. (1979) J. Bacteriol. 140, 320-326[Medline] [Order article via Infotrieve]
  25. Holmes, D. S., and Quigley, M. (1981) Anal. Biochem. 114, 193-197[Medline] [Order article via Infotrieve]
  26. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
  27. Sanger, F., Nicklen, S., and Coulson, A. R. (1977) Proc. Natl. Acad. Sci. U. S. A. 74, 5463-5467[Abstract]
  28. Pearson, W. R., and Lipman, D. J. (1988) Proc. Natl. Acad. Sci. U. S. A. 85, 2444-2448[Abstract]
  29. Belasco, J. G., Beatty, T., Adams, C. W., von Gabain, A., and Cohen, S. N. (1985) Cell 40, 171-181[Medline] [Order article via Infotrieve]
  30. Moralejo, P., Egan, S. M., Hidalgo, E., and Aguilar, J. (1993) J. Bacteriol. 175, 5585-5594[Abstract]
  31. Fuqua, W. C. (1992) BioTechniques 12, 223-225[Medline] [Order article via Infotrieve]
  32. Winans, S. C., Elledge, S. J., Krueger, J. H., and Walker, G. C. (1985) J. Bacteriol. 161, 1219-1221[Medline] [Order article via Infotrieve]
  33. Koonin, E. V., Tatusov, R. L., and Rudd, K. E. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 11921-11925[Abstract]
  34. Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G., and Blattner, R. (1994) Nucleic Acids Res. 22, 2576-2586[Abstract]
  35. Reverchon, S., Nasser, W., and Robert-Baudouy, J. (1991) Mol. Microbiol. 5, 2203-2216[Medline] [Order article via Infotrieve]
  36. Yura, T., Mori, H., Nagay, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., and Nakata, A. (1992) Nucleic Acids Res. 20, 3305-3308[Abstract]
  37. Weinert, T. A., Schaus, N. A., and Grindley, N. D. (1983) Science 222, 755-765[Medline] [Order article via Infotrieve]
  38. Segall, A., Mahan, M. J., and Roth, J. R. (1988) Science 241, 1314-1318[Medline] [Order article via Infotrieve]


Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.