From the Vienna Biocenter, Institute of Biochemistry
and Molecular Cell Biology, University of Vienna, A-1030 Vienna,
Austria, § Ludwig Boltzmann-Forschungsstelle für
Biochemie, A-1030 Vienna, Austria, the ¶ Institute of Biochemistry
and Molecular Biology, University of Berne, 3012 Berne, Switzerland,
and the
Institute of Microbiology, Academy of Sciences of
the Czech Republic, 14220 Prague, Czech Republic
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ABSTRACT |
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Eukaryotic initiation factor 3 (eIF3) consists of at least eight subunits and plays a key role in the formation of the 43 S preinitiation complex by dissociating 40 and 60 S ribosomal subunits, stabilizing the ternary complex, and promoting mRNA binding to 40 S ribosomal subunits. The product of the Saccharomyces cerevisiae RPG1 gene has been described as encoding a protein required for passage through the G1 phase of the cell cycle and exhibiting significant sequence similarity to the largest subunit of human eIF3. Here we show that under nondenaturing conditions, Rpg1p copurifies with a known yeast eIF3 subunit, Prt1p. An anti-Rpg1p antibody co-immunoprecipitates Prt1p, and an antibody directed against the Myc tag of a tagged version of Prt1p co-immunoprecipitates Rpg1p, demonstrating that both proteins are present in the same complex. A cell-free translation system derived from the temperature-sensitive rpg1-1 mutant strain becomes inactivated by incubation at 37 °C, and its activity can be restored by the addition of the Rpg1-containing protein complex. Finally, the rpg1-1 temperature-sensitive mutant strain shows a dramatic reduction of the polysome/monosome ratio upon shift to the restrictive temperature. These data show that Rpg1p is an authentic eIF3 subunit and plays an important role in the initiation step of translation.
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INTRODUCTION |
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Initiation of protein synthesis is a complex process requiring the participation of several factors promoting (a) the association of initiator tRNA with the 40 S ribosomal subunit in order to form the 43 S preinitiation complex; (b) the melting of secondary mRNA structures; (c) binding of the mRNA to the 43 S preinitiation complex; and (d) the addition of the 60 S ribosomal subunit to assemble an 80 S ribosome at the initiation codon (reviewed in Ref. 1). At this point, the initiation step of translation is accomplished, and the ribosome is committed to the elongation process. Up to now at least 10 proteins called initiation factors have been implicated in the initiation of protein synthesis (2). Among these initiation factors (eIFs),1 eIF3 represents the largest and the most complex one.
eIF3 is thought to be involved in the dissociation of the 80 S ribosome into 40 and 60 S subunits, to bind to 40 S subunits (3-5), and thereby to act as an anti-association factor hand in hand with eIF6 (5-8). Furthermore, eIF3 prevents dissociation of the Met-tRNAi·eIF2·GTP ternary complex caused by the addition of RNA (9), stabilizes the ternary complex by binding to the 40 S subunit (3, 5), and is required for mRNA binding to 40 and 80 S ribosomes (3, 5). eIF3 binds to eIF4F via the eIF4G subunit (10) and to eIF4B (11) and probably also interacts with eIF4A (12-14). eIF4F has been referred to as a protein complex composed of three subunits, eIF4G, eIF4A, and eIF4E, and was shown to be responsible for binding of the capped end of mRNA, melting of mRNA secondary structure, and binding to the 43 S preinitiation complex (1, 15, 16). This indicates that eIF3 may be the major factor orchestrating the accurate positioning of mRNA for binding to the 40 S subunit and subsequent recognition of the initiation AUG codon (6). Nevertheless, the timing of all translation initiation events as well as their coordination are only poorly understood. Thus, a characterization of not yet described proteins involved in that process can bring more light to our understanding of the mechanism of the initiation step of protein synthesis.
Mammalian initiation factor 3 is composed of at least 10 nonidentical subunits (17). It has been demonstrated that mammalian eIF3 directly interacts with the eIF4G subunit of eIF4F (10) and that the largest subunit of eIF3, p180, interacts with eIF4B (11). In addition, it has been proposed that p180 is necessary for binding and stabilization of the ternary complex (9). Thus, it seems that p180 plays a key role in all functions promoted by mammalian eIF3, resulting in formation of the 43 S mRNA complex. Only recently, mammalian p180 has been cloned and characterized (6). It shares significant sequence similarity with Saccharomyces cerevisiae, Caenorhabditis elegans, and Nicotiana tabacum proteins, which were suggested to probably represent the corresponding subunits of eIF3 in these organisms.
Yeast eIF3 comprises eight subunits with apparent molecular masses ranging from 16 to 135 kDa (18). Up to now, only four yeast homologues of mammalian eIF3 subunits have been identified. These are Prt1p, which exhibits a defect in Met-tRNAi binding to the 40 S ribosomal subunit in a corresponding conditional lethal mutant (19); Nip1p, originally identified as a protein required for efficient nuclear transport in yeast (20); Tif34p, a protein required for the assembly and maintenance of the eIF3 complex (21, 22); and Tif35p, a putative RNA-binding protein (22). These proteins are homologous to the human eIF3 subunits p110, p116, p36, and p44, respectively. The S. cerevisiae genome encodes one additional protein, p110, homologous to the human eIF3 subunit p180, which has not yet been identified and characterized. Yeast eIF3 also contains two previously described proteins, Gcd10p (23), and Sui1p (24), but corresponding homologues are not found in mammalian eIF3 (17). Nevertheless, the fact that the yeast factor can replace mammalian eIF3 in the methionyl-puromycin synthesis assay indicates strong conservation of function (18). Hence, the accurate definition of the subunit composition of yeast eIF3 and the description of subunit functions should also help to improve our understanding of mammalian eIF3 functions in the initiation of translation.
The essential S. cerevisiae gene RPG1 encoding a polypeptide with a calculated molecular mass of approximately 110 kDa has been described as being required for passage through the G1 phase of the cell cycle. Elutriated cells of the temperature-sensitive rpg1-1 mutant arrested in the early G1 phase of the first cell cycle and ceased cell growth when shifted to restrictive conditions (25). The Rpg1p amino acid sequence is 29% identical to that of p180. Here we report that S. cerevisiae Rpg1p is the functional homologue of the mammalian p180 protein, i.e. a subunit of yeast eIF3.
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EXPERIMENTAL PROCEDURES |
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Yeast Strains, Media, and General Techniques--
The
following strains were used in the work reported here: W303,
MATa, ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15,
ura3; YLV314U, MATa,
ura3::URA3::rpg1-1, rpg1-2::TRP1,
ade2-1, can1-100, leu2-3,112, his3-11,15 (25); YLV314L;
MATa, leu2-3,112::LEU2::rpg1-1, rpg1-
2::TRP1, ade2-1, can1-100, his3-11,15, ura3;
ABYS, MATa, pra1, prb1, prc1,
prs1, ade 2-1 (26); P501-1, MATa,
his6, ura3, prt1-1 (26); P501-1C,
MATa, his6, ura3, prt1-1,
YCpLVPRT1-Myc; YLVP10, MATa, ade2-1, trp1-1,
can1-100, leu2-3,112, his3-11,15, ura3, pRSLVPRT1-Myc; YLV3148,
MATa, leu2-3,112::LEU2::rpg1-1, rpg1-
2::TRP1, ade2-1, can1-100, his3-11,15,
ura3, YCpLV0318; YLV3148g, MATa,
leu2-3,112::LEU2::rpg1-1,
rpg1-
2::TRP1, ade2-1, can1-100, his3-11,15, ura3,
YCpLV718; YLV041, MATa,
ura3::URA3::MET3-RPG1, ura3,
rpg1-
1::LEU2, ade2-1, trp1-1, can1-100,
leu2-3,112, his3-11,15, YLV418, MATa,
ura3::URA3 ::MET3-RPG1, ura3,
rpg1-
1::LEU2, ade2-1, trp1-1, can1-100, leu2-3,112,
his3-11,15, YCpLV0318; YLV4180g, MATa,
ura3::URA3 ::MET3-RPG1, ura3,
rpg1-
1::LEU2, ade2-1, trp1-1, can1-100, leu2-3,112,
his3-11,15, YCpLV718; JJ-1A, MATa, arg1, thr1; JJ-1C,
MAT
, arg1, thr1. Strains were grown and subjected to genetic manipulations as described by Sherman et al.
(27).
Plasmid and Strain Constructions-- To construct strain YLV314L, a SacI-PstI fragment from YCpLV14 carrying the temperature-sensitive allele rpg1-1 (25) was inserted into the multiple cloning site of YIplac128 (28) to construct plasmid YIpLV314L. This plasmid was digested with BstEII and introduced into the diploid strain YLV03 (25) to produce the haploid strain YLV314L after sporulation and dissection of tetrads. Integration of the rpg1-1 allele into the LEU2 locus was confirmed by Southern blot analysis.
To regain the ura3 selection marker, cells of strain YLV04 (25) were grown on SC medium lacking methionine and supplemented with 0.1% 5-fluoroorotic acid. Following sequential streaking on four plates, a strain was obtained that retained the MET3-RPG1 fusion gene, but was auxotrophic for uracil (YLV041). pRSLVPRT1-Myc is a CEN6, HIS3 plasmid that contains the PRT1-Myc gene under its endogenous promoter. This plasmid was constructed in two steps. First, a PRT1 fragment generated by (PCR) using S. cerevisiae DNA as a template was digested using SalI-SacI PCR-incorporated artificial sites (a 5' SalI site at positionAssembly of the Full-length cDNA of p180-- To assemble the full-length sequence of p180, i.e. the cDNA encoding the human homologue of RPG1, a 4.75-kb MluI fragment containing the 3' coding region of p180 (nucleotides 564-4148) extended by 250 nucleotides of the flanking sequence was isolated from pUC19-3. The fragment was inserted into the MluI restriction site (at position 564 of the p180 nucleotide sequence) of pUC19-1 containing the 5'-end of the p180 sequence (nucleotides 1-574). The resulting plasmid carrying the full-length sequence of p180 was named pUCLVp180. Both pUC19-1 and -3 plasmids were kindly provided by Dr. Keith R. Johnson (University of Toledo, Toledo, OH).
For the construction of YCpLV018, a 4.6-kb CelII--SnaBI fragment containing the full-length p180 from pUCLVp180 was blunt-ended and inserted into a blunt-ended BamHI restriction site of YCpLV00 (25) producing the plasmid expressing p180 under control of the RPG1 promoter. A SacI-SalI fragment of YCpLV018 was ligated into SacI-SalI restriction sites of YCplac33 (28), resulting in YCpLV0318, which differs from the former plasmid merely in the selective marker URA3 instead of TRP1. YCpLV0318 was introduced into the YLV314L and YLV041 strains, resulting in strains YLV3148 and YLV418, respectively. For the construction of YCpLV718, a PCR-generated 5'-coding region of p180 (nucleotides 1-600) using YCpLV018 as a DNA template was digested with BamHI (PCR-incorporated artificial site immediately in front of the p180 ATG) and NcoI (endogenous restriction site at position +441 of the p180 nucleotide sequence). Then both a 4.2-kb NcoI-SalI fragment containing the 3'-coding region of p180 (nucleotides 441-4148) extended by 455 nucleotides of the flanking sequence and the former PCR-generated fragment were ligated into BamHI-SalI restriction sites of the 6.8-kb plasmid K2082 (provided by Dr. K. Nasmyth), yielding YCpLV718, which expresses p180 under control of the GAL1 promoter. The plasmid was introduced into strains YLV314L and YLV041 yielding strains YLV3148g and YLV418g, respectively.Mating Efficiency and -Pheromone Sensitivity
Assays--
Quantitative mating assays were performed as follows. The
cells were grown overnight at room temperature to mid-log phase, washed
once with sterile water, and resuspended in YPD medium (pH 4.7). Cells
(107) of both wild-type and rpg1-1 mutant
strains were mixed with an equal number of cells of mating tester
strains JJ-1A or JJ-1C; and mixtures were brought to a final volume of
1 ml; and cells were gently propagated for 4 h at either
permissive or restrictive temperature. Cells (104) from
mating samples were then plated onto minimal media without amino acids
to select for diploids and were incubated for 2 days. Subsequently, the
plates were scored for single colonies.
[3H]Leucine Labeling Experiments-- These were performed as described previously (30). Briefly, mutant cells of strain YLV314U were pregrown to mid-log phase in SC medium containing a reduced amount (25 mg/liter) of leucine (SC+ rLEU). The prewarmed (37 °C) and room temperature (25 °C) SC+ rLEU media were inoculated to a density of 5 × 106 cells/ml and incubated at the permissive or restrictive temperature for 24 h. Then cells from both incubations were split into two parts. Cells from one part were resuspended at a density of 5 × 107 cells/ml in 5 ml of prewarmed (37 °C) SC+ rLEU plus 10 µCi of [3H]leucine medium (hereafter called SC+[3H]LEU) and assayed for incorporation of the labeled amino acid at 37 °C. The second sample was manipulated similarly but resuspended in 25 °C SC+[3H]LEU medium and assayed at 25 °C. In the other set of experiments, YLV314U cells were propagated overnight in SC+ rLEU medium at room temperature, resuspended in SC+[3H]LEU medium, and, after 2 h of incubation at 25 °C, shifted to restrictive temperature and assayed.
After incubation, samples were processed as follows. 10 µg/ml of cycloheximide was added to each sample; then cells were isolated by centrifugation, washed once with TE buffer (20 mM Tris-HCl, pH 7.5), mixed with 600 µl of glass beads (0.45-0.50-mm diameter), brought to a final volume of 1 ml of TE buffer, and homogenized in a Braun homogenizer. After centrifugation at 10,000 × g, aliquots of clear supernatant were counted in a Packard scintillation counter. Radioactivity incorporated into protein was determined after applying equal aliquots onto Whatman DE81 filters, drying, incubating for 5 min in 20% trichloroacetic acid, and boiling for 5 min. Samples were counted in a scintillation counter using Ecolume (ICN) scintillation mixture after washing them twice with 20% trichloroacetic acid, washing once with 95% EtOH, washing once with pure acetone, and drying.Purification of the eIF3 Complex-- eIF3 was prepared from S. cerevisiae strain ABYS essentially as described previously (26).
Western Blot Analysis-- Samples containing eIF3, crude cell extracts, or immunoprecipitates were fractionated by SDS-PAGE (31) using the Mini Protean system (Bio-Rad) and immunoblotted to nitrocellulose (32). Immunodetection of proteins was carried out using either polyclonal (25) or monoclonal (33) antibodies directed against Rpg1p and either polyclonal rat anti-Prt1p antibody (26) or anti-Myc hybridoma supernatant antibody. As a secondary antibody, anti-rabbit, anti-rat, or anti-mouse IgG antibodies conjugated with alkaline phosphatase or with horseradish peroxidase, respectively, were used. Proteins were visualized either by using alkaline phosphatase substrates (nitro blue tetrazolium and 5-bromo-4-chloro-3-indolylphosphate) in AP buffer (100 mM Tris-HCl, 100 mM NaCl, 5 mM MgCl2, pH 9.5) or as described in the ECL manual (Amersham Pharmacia Biotech). The preparation of crude cell extracts used is described below. For maximum sensitivity, the gels were stained using a silver staining protocol (34).
The extract from human cells was prepared as follows. U937 promonocytes were cultured in RPMI medium containing 10% fetal serum and lysed in lysis buffer containing 10 mM Tris-HCl (pH 7.05), 50 mM NaCl, 30 mM NaPPi, 50 mM NaF, 2 mM EDTA, 1% Triton X-100, 1 mM phenylmethylsulfonyl fluoride, 0.1 mM sodium vanadate, 1 µg/ml pepstatin, and 3 µg/ml aprotinin. Extracts were cleared by centrifugation at 4,000 × g at 4 °C for 8 min.In Vitro Translation-- The following yeast strains were used for the preparation of cell-free translation systems: P501-1, YLV314L, and W303. Preparation of the extracts, cell-free translation, and the preparation of total yeast RNA were performed as described previously (35, 36).
Polysome Profile Analysis--
To analyze polysome profiles,
strains W303 and YLV314L were cultured overnight in YPD at room
temperature to an A600 of 0.8. Samples of 20 ml
were centrifuged at 500 × g for 3 min and resuspended in an equal volume of YPD either kept at room temperature or prewarmed to 37 °C, after which the samples were incubated for 1 h at the temperatures indicated. Just 5 min before harvesting, 10 µg/ml cycloheximide was added to the cultures, followed by quick cooling. The
cells were collected by centrifugation, resuspended in ice-cold buffer
A (20 mM Tris-HCl, pH 7.5, 100 mM KCl, 5 mM MgCl2, 0.1 mM EDTA, 7 mM -mercaptoethanol, 1 mM dithiothreitol),
and homogenized by shaking with a Vortex mixer using glass beads. The
cell lysates were clarified by centrifugation at 4000 × g for 2 min at 4 °C. Samples of 2 A260 units of whole extracts were fractionated
on linear 5-40% sucrose gradients for 40 min at 260,000 × g. Fractionation of polysomes was done by upward
displacement while scanning at 260 nm with an ISCO detector.
Coimmunoprecipitations-- Crude extracts were prepared as described previously (25), except that buffer A (30 mM HEPES (pH 8.8), 100 mM KAc, 3 mM magnesium acetate, 1 mM dithiothreitol, 0.1% SDS, 1% Nonidet P-40, 1 mM 4-(2-aminoethyl)-benzene sulfonyl fluoride (Sigma)) supplemented with Complete Protease Inhibitor Mix tablets (Boehringer Mannheim) was used for lysis of the cells, and cell lysates were centrifuged at 3,000 rpm for 20 min at 4 °C. Antibodies were preincubated for 2 h with 50 µl of GammaBind-G Sepharose beads (Amersham Pharmacia Biotech) in buffer A, except in the case of the monoclonal anti-Rpg1p antibody, which was directly incubated with samples. Samples containing 5 mg of total protein in a final volume of 100 µl were incubated for 1 h with 30 µl of 1:1 slurry of GammaBind-G Sepharose beads in buffer A. The beads were collected by brief centrifugation and discarded to remove antigens that bound nonspecifically to G-protein-Sepharose. The remaining supernatants were mixed either with 50 µl of beads already preincubated with anti-Myc antibody or with monoclonal anti-Rpg1p antibody for 2 h. Subsequently, 50 µl of fresh beads in buffer A were added. All samples were incubated overnight at 4 °C on a rotating mixer. Samples were centrifuged briefly, and the supernatants were removed. The beads were washed five times with 400 µl of buffer A and were then mixed with 50 µl of SDS buffer, heated for 2 min at 95 °C, and spun down. The supernatants were analyzed by SDS-PAGE and immunoblotted as described above. Equivalent portions of the supernatants and the pellet fractions were analyzed to estimate the fraction of the proteins bound in immune complexes.
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RESULTS |
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Mating Ability of rpg1-1 Mutant and Sensitivity to
-Factor--
We demonstrated recently that the S. cerevisiae essential gene RPG1 is required for the
passage through the G1 phase (25). There are three points
in the yeast cell cycle where the further progression depends on active
protein synthesis, of which the G1 phase is the most
important (37). Mating of haploid S. cerevisiae cells of
opposite mating types also requires a functional translational mechanism. When it appeared to us that the RPG1 gene product
could be a homologue of the mammalian p180 (6), a subunit of eIF3, we
examined the mating ability of the rpg1-1 ts mutant at
permissive and restrictive temperature, to obtain further preliminary
evidence for an impairment of translation in the absence of functional Rpg1p. YLV314U mutant cells as well as wild-type W303 cells were propagated overnight at 25 °C, and a mating assay was then performed at either 25 or 37 °C. As shown in Table
I, the mating efficiency of the
rpg1-1 ts mutant is strikingly diminished (5-fold) even at
the permissive temperature compared with wild-type cells. The ability
to mate was completely lost in rpg1-1 cells when mating was
performed at the restrictive temperature. Mutant alleles of both mating
types yielded virtually the same results, consistent with the
assumption that protein synthesis of mutant cells might be
affected.
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The rpg1-1 ts Mutation Causes a Block of Protein Synthesis in Vivo-- The mating ability experiments are consistent with the assumption that translation is blocked in rpg1-1 cells at the restrictive temperature. To obtain more direct evidence, we examined the incorporation of radiolabeled amino acids into total protein of the rpg1-1 mutant cells. As shown in Fig. 1A, rpg1-1 mutant cells (strain YLV314U (25)) exhibit a severe block of leucine incorporation into protein upon shift to the restrictive temperature. We also tested leucine incorporation occurring in rpg1-1 cells incubated for 24 h at 37 °C and shifted back to 25 °C at time point 0 of the assay. This experiment revealed that the observed block of protein synthesis is reversible.
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Rpg1p Comigrates with Prt1p on Sucrose Gradients-- Rpg1p exhibits significant similarity at the amino acid level to the recently characterized largest subunit of the human eIF3, p180 (6, 17, 25). To address the question of whether Rpg1p is indeed one of the subunits of yeast eIF3, we first attempted to detect Rpg1p in a fraction containing highly purified eIF3 (26). Prt1p was previously identified as the second largest subunit of yeast eIF3 (18) and was therefore used as a specific marker for the presence of the eIF3 complex, which was enriched in a four-step purification (26). A ribosomal salt wash fraction prepared from wild-type strain ABYS was fractionated by DEAE-cellulose and phosphocellulose chromatography and by sucrose density gradient centrifugation. The optical density profile of the sucrose gradient showed a major peak of rapidly sedimenting material in fractions 10-12 (not shown). Sucrose gradient fractions 8-14 were further analyzed by SDS-PAGE and subsequently by Western blotting. Silver staining of the gel revealed the copurification of five polypeptides in fractions 10-12 corresponding to five proteins with molecular masses of approximately 120, 80, 75, 40, and 30 kDa, previously identified as subunits of the yeast eIF3 (26) (Fig. 2A, arrows). As demonstrated by Western blotting (Fig. 2B), a polypeptide migrating as a protein of 120 kDa reacts with the monoclonal anti-Rpg1p antibody. The intensity of the signal obtained with the anti-Rpg1p monoclonal antibody, like the eIF3 subunit, peaks at fraction 11. Since the anti-Rpg1p antibody recognizes exclusively one band of about 120 kDa corresponding to Rpg1p (110 kDa calculated molecular mass) (25), the additional immunoreactive band detected as a 100-kDa protein (arrow) appears to represent a degradation product of Rpg1p, as was suggested already (26). The same probably applies to the 50-kDa immunoreactive band observed in the phosphocellulose (PC) fraction and fraction 11 of the sucrose gradient (Fig. 2B, arrowhead). Subsequent decoration of the same blot using polyclonal rat anti-Prt1p antibody showed that Rpg1p and Prt1p exhibit virtually the same mass distribution over sucrose gradient fractions 9-14 (Fig. 2C). Since the sucrose density gradient fractions composed of five polypeptides with defined molecular weights (fractions 10-12 (Fig. 2A, arrows)) have been proposed to represent a core complex of yeast eIF3 (17), our results strongly suggest that Rpg1p is the large subunit of this core complex.
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Rpg1p Coimmunoprecipitates with Prt1p, a Known Subunit of eIF3-- To provide additional evidence for Rpg1p being a subunit of yeast eIF3, we asked whether it could be coimmunoprecipitated with the Prt1 protein (18, 26). Prt1p was already shown to coimmunoprecipitate with Gcd10p (23) and Sui1p (24) and to interact directly with Tif34p (22). A Myc-tagged version of Prt1p and anti-Myc antibodies were used for Prt1p immunodetection. The tagged protein was shown to complement the ts defect of the prt1-1 ts mutant (strain P501-1), demonstrating that it is functional (result not shown). Crude extracts from strains expressing a tagged or an untagged version, respectively, of Prt1p were prepared and used for immunoprecipitation experiments with antibodies directed against Rpg1p or Prt1-Mycp. The resulting immunocomplexes were separated by SDS-PAGE and probed by immunoblot analysis using either anti-Rpg1p or anti-Myc antibodies. Both antibodies immunoprecipitated similar amounts of Rpg1p and Prt1-Myc fusion protein from the crude extracts as shown in Fig. 3, lanes 5 and 6. In control experiments, anti-Myc antibody immunoprecipitated detectable amounts of neither PRT1 nor RPG1 gene products of the Prt1p-untagged strain (Fig. 3, lane 8). Similarly, in an immunoprecipitate obtained with anti-Rpg1p antibody, untagged Prt1p was not detectable by anti-Myc antiserum (Fig. 3, lane 7). The finding that monoclonal anti-Rpg1p antibody coimmunoprecipitates Prt1p, a known subunit of yeast eIF3, and that anti-Prt1-Mycp antibody coimmunoprecipitates tagged Rpg1p provides direct evidence that Rpg1p is an integral component of yeast initiation factor 3.
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Rpg1p Is Required for the Initiation of Protein Synthesis-- The finding that the RPG1 gene is essential for cell growth and mating in addition to the fact that it encodes one of the eIF3 subunits, led us to investigate whether Rpg1p is required for translation initiation. It has been shown that Prt1p (26), Tif34p (21, 22), Sui1p (24, 39), and Gcd10p (23), all yeast eIF3 subunits, constitute essential functional components of the yeast translational machinery. We approached this question using an in vitro translation assay based on cell-free systems derived from ts strains deficient in translation initiation when the extract of cells is briefly preincubated at 37 °C (26). We first tested the Rpg1p-Prt1p-containing protein complex isolated as described above (Fig. 2) for biological activity in a cell-free system derived from strain P501-1. When the cell-free system derived from this prt1-1 mutant was heated for 30 s at 37 °C, the methionine incorporation was dramatically diminished in the absence of endogenous RNA or after the addition of total yeast RNA compared with the nonheated extract (Fig. 4D versus 4A). The addition of the sucrose gradient fraction 11 (Fig. 2A) containing eIF3 restored the initiation of translation (about 6-fold stimulation) (Fig. 4D), while translation in the nonheated extract was stimulated only 1.5-2-fold (Fig. 4A). These results are consistent with results reported previously (26) and show that Rpg1p in the protein complex is active as a translation factor in vitro.
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Human eIF3-p180 Cannot Replace Its Yeast Counterpart RPG1 in Vivo-- Many mammalian factors can substitute for their yeast counterparts (2), indicating functional homology between translational factors. Importantly, it has been shown that yeast eIF3 could substitute functionally for human eIF3 in a methionyl-puromycin synthesis assay with mammalian components, which mimics the formation of the first peptide bond (18). Only recently, a human cDNA homologue of the RPG1 gene has been cloned and named p180 (6). S. cerevisiae Rpg1p and human p180 share 29% sequence identity and 54% similarity. The N-terminal regions of both homologues show more similarity than C-terminal ones (6, 25). An investigation of the functional relatedness of both homologues could provide additional evidence for evolutionary conservation of the translation process from the unicellular yeast to humans.
To investigate whether the human p180 cDNA can replace the yeast gene in vivo, we first had to assemble the 5.3-kb full-length p180 cDNA clone from two incomplete p180 cDNAs and place it behind either an endogenous RPG1 or the GAL1-10 promoter. Since RPG1 is an essential gene, we were able to use the no-growth phenotype of both the Rpg1p depletion and the rpg1-1 ts mutant strains for a complementation test. Human p180 cDNA driven from either the RPG1 or the GAL1-10 promoter failed to substitute for RPG1 in the Rpg1p-depleted strains (YLV0418 and YLV0418g strains) and to rescue the growth of the rpg1-1 ts mutant strains (YLV3148 and YLV3148g strains) at the restrictive temperature (data not shown). Northern blot analysis with the human p180 cDNA probe revealed that the human p180 is transcribed well (data not shown). The accumulation of human p180 in S. cerevisiae was tested by Western immunoblot analysis using an anti-p180 hybridoma supernatant antibody (a kind gift of Dr. Keith R. Johnson) (Fig. 6). Human p180 is expressed under control of the GAL1-10 (lane 2) as well as the RPG1 promoter (lane 4). No significant cross-reactivity was observed between the polyclonal anti-Rpg1p antibody and human p180 (not shown). Similarly, the monoclonal anti-p180 antibody did not recognize the yeast Rpg1p (Fig. 6). These data demonstrate that the lack of complementation of the rpg1-1 mutation by human p180 is not caused by insufficient expression of this protein in yeast.
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DISCUSSION |
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The essential S. cerevisiae gene RPG1 has
recently been shown to be required for the passage through the
G1 phase of the cell cycle. Depletion of Rpg1p and/or
inactivation of the ts Rpg1 mutant protein at the restrictive
temperature resulted in cessation of cell growth and the accumulation
of mutant cells in the G1 phase (25). By virtue of data
base searches, S. cerevisiae Rpg1p has been shown to share
significant sequence similarities with proteins from the nematode
C. elegans, the plant N. tabacum, and the mammals Mus musculus and humans (6, 25). Just recently, the human cDNA homologue (p180) of the RPG1 gene has been
identified as the largest subunit of the eukaryotic translation
initiation factor 3. Since S. cerevisiae Rpg1p and human
p180 share 29% sequence identity and 54% similarity, it was suggested
that yeast RPG1 constitutes the corresponding subunit of
yeast eIF3 (6). The failure of mutant cells lacking functional
RPG1 to pass through the G1 S transition and
the report on the human homologue of RPG1 led us to examine
the involvement of RPG1 in translation. Here we present
several lines of evidence showing that Rpg1p indeed represents a
subunit of yeast translation initiation factor 3 required for the
initiation of translation.
The passage through the regulatory step of the G1 phase
called START requires efficient protein synthesis (37). Translational mechanisms have also been suggested to play a regulatory role in the
genetic coordination of growth and cell division (41). Depending on
growth conditions, eIF3 influences Cln3p synthesis (and probably not
only Cln3p) and thus, as a consequence, controls the G1 S transition (41). Furthermore, protein synthesis has been shown to be
essential for the progression of the mating pheromone pathway (42). We
showed here that rpg1-1 ts mutant cells are defective in
incorporation of a labeled amino acid into protein and fail to mate and
to respond to
-pheromone at the restrictive temperature.
These results are consistent with the previous finding that a
lack of a functional Tif34p, another yeast eIF3 subunit, results in
G1 arrest and in an inability to mate and to respond to
-factor (22). They further illustrate the dependence of the mating
pheromone pathway on functional protein synthesis.
Our monoclonal antibody against Rpg1p cross-reacted with a polypeptide with an apparent molecular mass of 120 kDa, which comigrates on sucrose density gradients with Prt1p and three other subunits together referred to as the core complex of eIF3 (Fig. 2). We have shown that this fraction possesses the biochemical activity ascribed to eIF3, e.g. the restoration of translation in an extract in which an endogenous eIF3 subunit has been inactivated (Fig. 4) (26). It should be noted that the RPG1 gene product was reported to be a protein ranging in molecular mass between 120 and 135 kDa (25, 26) whereas the calculated molecular mass derived from the amino acid sequence is 110 kDa. Likewise, the reported molecular mass of human p180 according to SDS-PAGE varies between 170 and 180 kDa (3, 6, 43) and also exceeds that deduced from its sequence (166 kDa).
The direct interaction between hPrt1 and the p180 subunit of mammalian eIF3 has already been demonstrated using Far Western analysis (40). p180 was also reported to be the subunit of mammalian eIF3 through which eIF3 interacts with eIF4B (6). We have shown here that Rpg1p could be specifically coimmunoprecipitated with the Prt1p subunit of eIF3 using antibodies against Rpg1p and the Myc tag fused to Prt1p (Fig. 3). This indicates but does not prove that Rpg1p interacts directly with Prt1p. Furthermore, S. cerevisiae Rpg1p may possess more interacting partners than only Prt1p. The precise investigation of these interactions is needed to improve our understanding of the roles of particular eIF3 subunits in the initiation of translation.
Incubation of the rpg1-1 ts mutant at the restrictive temperature rapidly blocks the initiation of protein synthesis as assessed from the polysome profile carried out 1 h after the upshift (Fig. 5). However, such an experiment cannot provide information on an exact role of RPG1 in the initiation process; hence, this role still remains to be elucidated.
Many translation initiation factors have been tested for their ability to functionally replace the corresponding yeast factors (for a review, see Ref. 2). Up to now, only three translation components are known, namely eIF4A (2), hPrt1 (40), and human eIF3-p36 (21), which failed to substitute for their yeast counterparts. We show here that the human eIF3-p180 cDNA is also not able to replace its yeast homologue, RPG1, even when the protein is overexpressed (Fig. 6). Since the exact roles of both proteins are still unclear, it is rather difficult to speculate about the reasons why human p180 is unable to fulfill functions of the yeast Rpg1p in vivo. One possible reason might be the inability of human p180 to incorporate efficiently into the yeast eIF3 multiprotein complex. The failure of three human eIF3 subunits to complement is somewhat surprising in light of the fact that yeast eIF3 functions in a mammalian methionyl-puromycin assay system (18). This finding demonstrates the substantial diversity, apart from significant conservation, of translation initiation between these two evolutionary fairly distant species.
Only recently, mammalian eIF3 has been purified from rabbit reticulocyte lysates using an assay that specifically measures its ability to stimulate the binding of the ternary complex to 40 S ribosomal subunits (8). Strikingly, these eIF3 preparations lacked the 180-kDa polypeptide, which was present in mammalian eIF3 preparations purified in other laboratories based on translation assays (3, 43). The finding that an eIF3 preparation lacking p180 was fully competent to stimulate Met-RNAi binding to 40 S ribosomal subunits led the authors to speculate that the p180 subunit might not be a true subunit of mammalian eIF3 but might rather associate with eIF3 in a discrete step in the initiation process (8). Similarly, the yeast homologue of p180, Rpg1p, was not observed as an abundant eIF3 component in all S. cerevisiae eIF3 preparations described up to now (18, 26, 44). Hershey et al. (44) mentioned that they have seen the p110 component of eIF3 in some, but not all, of their eIF3 preparations. They suggested p110 to be an eIF3 subunit (44) by virtue of peptide sequencing of the p21 eIF3 component (18), which exactly matched the sequence of p110 (open reading frame YBR079c, Saccharomyces Genome Database). They named the corresponding complete open reading frame TIF32 (44). This gene is identical with the recently described gene RPG1 (25). Hershey et al. (44) concluded that the previously identified eIF3 component p21 probably represents an Rpg1p/Tif32p degradation product (44). The absence of Rpg1p/Tif32p in some of their eIF3 preparations might mean that either Rpg1p/Tif32p is rather unstable under conditions of the eIF3 complex preparation used by this laboratory or that Rpg1p/Tif32p exerts some specific function(s) outside of the eIF3 complex and under certain conditions is not associated with or preferentially dissociates from the eIF3 complex. The absence of human p180 in the preparation of human eIF3 by Maitra and co-workers (8) could be consistent with this latter interpretation. Other open questions concern the finding that some noncore eIF3 subunits in yeast do not have corresponding mammalian homologues and vice versa (17). More experiments are needed to reveal both the true subunit composition and details of the functions of eIF3 in yeast and mammals.
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ACKNOWLEDGEMENTS |
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We thank Keith R. Johnson for the pUC19
plasmids containing cDNAs of p180 as well as for the anti-p180
hybridoma supernatant antibody, Pavel Kovarik for the extract of U937
human cells, and Kim Nasmyth for plasmid K2082. We also thank Gustav
Ammerer, Pavel Kovarik, Michael Altmann, Jan Paleek,
Vladimír Reiser, and Gerard Griffioen for helpful advice and
support throughout this project. The technical assistance by Harald
Nierlich is gratefully acknowledged.
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FOOTNOTES |
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* This work was supported by a grant from the Bundesministerium für Wissenschaft und Verkehr (Vienna, Austria) (to H. R.), by Swiss National Foundation Grant 31-45528.95 (to H. T.), and by the Grant Agency of the Czech Republic (Prague, Czech Republic) Grant 204/96/1368 (to J. H.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
** To whom correspondence should be addressed: Institut für Biochemie und Molekulare Zellbiologie, Dr. Bohrgasse 9, A-1030 Wien, Austria. Tel.: 431-4277-52815; Fax: 431-4277-7528; E-mail: hr{at}abc.univie.ac.at.
The abbreviations used are: eIF, eukaryotic translation initiation factor; PCR, polymerase chain reaction; PAGE, polyacrylamide gel electrophoresis; ts, temperature-sensitive; kb, kilobase pair(s).
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REFERENCES |
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