Routing and Processing of Lactase-Phlorizin Hydrolase in Transfected Caco-2 Cells*

Joke OuwendijkDagger , Wilma J. M. PetersDagger , Rinske A. van de VorstenboschDagger , Leo A. GinselDagger , Hassan Y. Naim§, and Jack A. M. FransenDagger

From the Dagger  Department of Cell Biology and Histology, University of Nijmegen, P. O. Box 9101, 6500 HB Nijmegen, The Netherlands and § Department of Physiological Chemistry, Hannover School of Veterinary Medicine, Bünteweg 17, D-30559 Hannover, Germany

    ABSTRACT
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Abstract
Introduction
Procedures
Results & Discussion
References

Human lactase-phlorizin hydrolase (LPH) is a digestive enzyme that is expressed in the small intestinal brush-border membrane. After terminal glycosylation in the Golgi apparatus, the 230-kDa pro-LPH is cleaved into the 160-kDa brush-border LPHbeta and the 100-kDa profragment (LPHalpha ). Since LPHbeta is not transport-competent when it is expressed separately from LPHalpha in COS-1 cells, it was suggested that LPHalpha functions as an intramolecular chaperone. What happens to LPHalpha after cleavage is still unclear.

To analyze and localize LPHalpha in polarized epithelial cells, wild type and tagged LPH were stably expressed in Caco-2 cells. In tagged LPH, a vesicular stomatitis virus epitope tag was inserted into the LPHalpha region. Wild type and tagged proteins were processed at similar rates, and both cleaved LPHbeta forms were expressed at the apical cell surface. Pro-LPH was recognized by antibodies against LPH, a profragment epitope and the vesicular stomatitis virus tag. LPHalpha alone, however, could not be recovered by these antibodies. Our data suggest that LPHalpha is degraded immediately after cleavage.

    INTRODUCTION
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Abstract
Introduction
Procedures
Results & Discussion
References

Human lactase-phlorizin hydrolase (LPH)1 is a disaccharidase that is localized at the microvillar membrane of epithelial cells in the small intestine. It is responsible for the hydrolysis of lactose, the main carbohydrate in mammalian milk.

LPH is synthesized as a 1927 amino acid precursor, prepro-LPH. The first 19 amino acids of this precursor form the signal sequence that is cleaved off in the endoplasmic reticulum. The remaining 1908 amino acids form the pro-LPH, which is complex glycosylated on its way through the Golgi apparatus. A subsequent cleavage between Arg-734 and Leu-735 (1) takes place after export from the trans-Golgi and results in the 160-kDa LPHbeta and a stretch that is called the profragment or LPHalpha (2). LPHbeta is expressed at the apical membrane, where it is trimmed by luminal trypsin in the small intestine to the intestinal form of LPHbeta . In contrast to LPHbeta , the fate of LPHalpha after cleavage is not clear. In Fig. 1, the processing of LPH is illustrated.


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Fig. 1.   Cartoon of the processing of LPH. LPH is synthesized as prepro-LPH. Removal of the signal sequence results in pro-LPH. After complex glycosylation in the Golgi apparatus, pro-LPH is cleaved into LPHalpha (profragment) and LPHbeta (mature LPH). LPHbeta is expressed at the brush border, but the fate of LPHalpha remains unknown. In intestinal cells, LPHbeta is further trimmed by luminal proteases to intestinal LPHbeta .

Prepro-LPH consists of four homologous regions between the signal peptide and the hydrophobic stretch at the C terminus. In Fig. 2, prepro-LPH is schematically drawn. The first two regions, I and II, are localized in LPHalpha . None of them possess the Glu residue that was shown to be essential for the active sites (3). Indeed, LPHalpha is enzymatically not active toward lactose when expressed separately in COS cells or isolated after trypsin cleavage of the pro-LPH expressed in COS cells (4). Regions III and IV both contain the Glu residue and are responsible for the disaccharidase activity of LPHbeta (3). Because of the homology between LPHalpha and LPHbeta , and because LPHalpha comprises 40% of the primary translation product, various functions have been suggested for LPHalpha (2, 5).


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Fig. 2.   Schematic overview of several structural features in LPH. The encircled Glu residues depict the essential residues in the active sites. The homologous regions are denoted by roman numerals. The introduction site of the VSV tag is depicted as well. The alpha /beta cleavage site is located at Arg-734/Leu-735, intestinal LPH has Ala-869 at its N terminus.

LPHalpha has previously been isolated from intestinal biopsy samples using an antibody directed against the 12 amino acids directly after the signal peptide (Ser-20-Thr-31) (2). The molecular mass of the immunoprecipitated profragment in these experiments was ~100 kDa (2). Because none of the used glucosaminidases (endo-N-acetylglucosaminidase H (endo H) and endo-N-acetylglucosaminidase F/glycopeptidase F (endo F/GF)) influenced the apparent molecular weight, it was concluded that the 5 consensus N-glycosylation sites were not glycosylated (2), although some conflicting data exist (1). In addition it was shown that LPHalpha does not form a stable complex with LPHbeta after cleavage in intestinal biopsy specimens (2). These observations and further expression studies of the intestinal form of LPHbeta (from Ala-869 to the C terminus) in COS-1 cells lead to the hypothesis that LPHalpha functions as an intramolecular chaperone. Where exactly in the cell the cleavage takes places as well as what happens to LPHalpha after cleavage is still uncertain despite extensive published data (2, 5).

To track down LPHalpha after cleavage, we decided to express and analyze LPH in Caco-2 cells. These cells are able to express LPH endogenously, and to perform the cleavage between the alpha  and beta  domain of pro-LPH (6). Furthermore, we introduced a VSV tag into LPHalpha to generate additional recognition possibilities. Immunoprecipitation and localization studies were performed in Caco-2 cells that were stably transfected with wild type and VSV-tagged LPH cDNA. The results strongly suggest that the LPHalpha profragment is immediately degraded after cleavage in Caco-2 cells and therefore argue in favor of the intramolecular chaperone function.

    EXPERIMENTAL PROCEDURES
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Abstract
Introduction
Procedures
Results & Discussion
References

Antibodies-- HBB1/909 is an epitope-specific monoclonal antibody directed against LPH (6). This product of the hybridoma HBB1/909/34/74 was provided by Hans-Peter Hauri, Biozentrum der Universität Basel, Switzerland. V496 is a polyclonal antiserum directed against the first 12 amino acids following the signal sequence of prepro-LPH (2). P5D4 is a monoclonal antibody against a specific epitope in VSV-G-protein (7), the hybridoma was provided by Thomas Kreis, Dept. of Cell Biology, University of Geneva, Switzerland.

DNA Constructs-- To insert a VSV epitope tag into the LPH cDNA, the LPH cDNA (8) was cloned into a pBluescript KS+ vector in which the SstI site was removed. This construct was digested with SstI, blunted with T4 DNA polymerase (New England Biolabs, Beverly, MA) and treated with calf intestinal phosphatase (Boehringer Mannheim BV, Almere, The Netherlands). For the construction of a 43-base pair VSV-tag insert, coding for 11 amino acids of the epitope, two oligonucleotides (5'-GGAGATCTTATACAGACATAGAGATGAA-3' and 5'-GGGGGATCCCTTTCCAAGTCGGTTCATCTCTATGTCTGTA-3') were annealed and treated with Klenow (Boehringer Mannheim BV) in the presence of dNTPs to fill in the 5' overhangs. The double-stranded product was digested with BamHI and BglII, and the 5' overhangs were filled in by Klenow treatment. This blunted BamHI/BglII insert was ligated into the blunted SstI-digested LPH cDNA so that the reading frame remained intact. The orientation of the insert was determined by sequencing. Fig. 3 depicts the sequence of the insert and the flanking LPH sequences, as well as the amino acid sequence of the wild type LPH and the tag insert in this region. This construct, denoted LPHST, was cloned into a modified pSG5 (9) expression vector containing a puromycin resistance cassette (pSGpuro), which resulted in the pLPHST plasmid. Wild type LPH cDNA was cloned into pSGpuro as well (pLPHwt).


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Fig. 3.   Alignment of the amino acid and DNA sequences of wtLPH and LPHST around the introduction site of the VSV tag. Lines connect the corresponding amino acids. The box is drawn around the VSV tag sequence, which is shown in boldface type. Base pair numbering is given above the sequence, and amino acid numbering is given below.

To generate the pSGpuro vector the SalI fragment of the pKSpuro vector, which was kindly provided by Peter Laird (University of Southern California School of Medicine, Norris Comprehensive Cancer Center, Los Angeles, CA) (10) was inserted into the partially SalI digested pSG8 (a pSG5 vector (9) with a multiple cloning site).

Cell Lines-- Monkey kidney COS cells (ATCC CRL-1650) were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum and antibiotics. Caco-2 TC7 and PD10 cells (a gift from Dr. Monique Rousset, Ref. 11) were cultured in Dulbecco's modified Eagle's medium supplemented with 20% heat-inactivated fetal calf serum, 1% nonessential amino acids and antibiotics (all from Life Technologies, Inc.). All cell lines were cultured at 37 °C in a humidified 5% CO2 incubator.

Transfection and Selection-- COS-1 cells were transfected via electroporation as described before (12). Caco-2 cells were transfected by LipofectAMINE treatment. One or two days prior to transfection, 0.2 × 106 Caco-2 TC7 cells were seeded per 35-mm culture dish, so that on the day of transfection the culture was 60-80% confluent but not polarized. During the whole transfection procedure, no antibiotics were used. On the day of transfection, cells were washed twice with OptiMEM (Life Technologies, Inc.). Solution A, consisting of 2 µg of linearized DNA in 200 µl of OptiMEM, was gently mixed with solution B, consisting of 6 µl of LipofectAMINE (Life Technologies, Inc.) and 200 µl of OptiMEM. The mixture was incubated at room temperature for 30 min, and 1600 µl of OptiMEM was added. The resulting 2 ml. was added to the cells and incubated at 37 °C and 5% CO2 for 6 h. Cells were washed with normal Caco-2 medium and incubated in this medium for 24 h, refreshed, and incubated for another 24 h. Cells were trypsinized and seeded into 96-well plates at a density of 5 × 103 cells per well. After 24 h, selection medium was added, which consisted of normal Caco-2 medium with an (empirically determined) puromycin concentration of 13 µg/ml. After 1 week, wells with only one colony were selected. After another week, these colonies were trypsinized and cultured further in selection medium.

Metabolic Labeling and Immunoprecipitation-- Caco-2 cells or transiently transfected COS-1 cells were metabolically labeled with 100 µCi Tran35S-label (ICN Biomedicals) as described by Naim et al. (8). After the labeling period, the cells were scraped in lysis buffer (1% Triton X-100, 0.2% bovine serum albumin in 100 mM phosphate buffer, pH 8.0, containing 1 tablet of complete protease inhibitor mixture (Boehringer Mannheim BV) per 25 ml) and lysed at 4 °C for 1 h. For COS cells usually 1 ml of ice-cold lysis buffer was used for each 100-mm culture dish (about 2-4 × 106 cells). For Caco-2 cells, 0.5 ml was used per filter. Lysates were stored at -135 °C until use. Detergent extracts of cells were centrifuged for 1 h at 100,000 × g at 4 °C and the supernatants were immunoprecipitated as described by Schweizer et al. (13).

SDS-PAGE-- SDS-PAGE was performed according to Laemmli (14), and the apparent molecular weights were assessed by comparison with high molecular weight markers (Bio-Rad) run on the same gel. In some experiments, deglycosylation of the immunoprecipitates with endo H and endo F/GF (also known as PNGase F) (both from New England Biolabs) was performed prior to SDS-PAGE analysis as described before (15).

Trypsin Sensitivity Assay-- The sensitivity for proteatic digestion of LPH and LPHST was compared using a trypsin sensitivity assay. Transfected COS-1 cells were metabolically labeled and lysed. LPH and LPHST were immunoprecipitated (see above) using the HBB1/909 antibody. Precipitates were divided into several fractions and treated with 50 µg/ml trypsin at 37 °C for the indicated incubation times. Samples were subjected to SDS-PAGE on a 8% gel that was analyzed by fluorography.

Enzyme Activities-- Disaccharidase activities of immunoprecipitated LPHs were measured according to Dahlqvist (16) using lactose as a substrate. The method was essentially the same as described by Naim et al. (8). LPH was immunoprecipitated using the HBB1/909 antibody from lysates of 165-cm2 culture flasks of (transfected) Caco-2 cells. The precipitates were dissolved in 2 ml of phosphate-buffered saline containing 0.3% Triton X-100 of which 25-µl aliquots were used for the enzyme activity assay. The amount of precipitated LPH was estimated in 500-, 300-, 200-, and 100-µl aliquots on an SDS-PAGE gel with a bovine serum albumin concentration standard series run on the same gel; the gel was stained with Coomassie Brilliant Blue.

Immunofluorescence and Confocal Microscopy-- Cellular localization of expressed proteins in COS-1 cells and Caco-2 cells was studied with cells grown on coverslips. Cells were fixed with 3% paraformaldehyde and permeabilized with 0.1% Triton X-100. Immunolabeling was carried out using (as primary antibodies) monoclonal antibodies HBB1/909 against human LPH, P5D4 against the VSV epitope tag, and the polyclonal antibody V496 against the 12 amino acids directly after the signal sequence of pro-LPH. The secondary antibodies employed fluorescein isothiocyanate-conjugated goat anti-mouse or swine anti-rabbit IgG, Texas Red-conjugated goat anti-mouse or anti-rabbit IgG (all were from Boehringer Mannheim BV). Surface localization of proteins was assessed in transfected cells that were not fixed nor permeabilized. Labeling was carried out at 4 °C. Label was visualized using a Bio-Rad MRC1000 confocal scanning laser microscope using a double channel for fluorescein isothiocyanate and Texas Red or on a routine fluorescence microscope.

Immunoelectron Microscopy-- Ultrastructural localization studies were performed on transfected Caco-2 TC7 clones grown on a filter that had been confluent for 5 days. They were fixed with 1% paraformaldehyde and 0.1% glutaraldehyde in phosphate buffer (pH 7.3) for 1 h and stored until use in 1% paraformaldehyde. Filters were stacked in 10% gelatin and fixed in 1% paraformaldehyde for 24 h. Ultrathin cryosectioning was performed as described before (13, 17). Sections were incubated with the monoclonal antibodies HBB1/909 against human LPH or P5D4 against the VSV epitope tag, followed by a rabbit polyclonal serum against mouse IgG (Dako A/S, Glostrup, Denmark) and protein A complexed with 10 nm gold (17). Electron microscopy was performed with a JEOL 1010 electron microscope.

    RESULTS AND DISCUSSION
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Procedures
Results & Discussion
References

Processing of wtLPH and LPHST in COS-1-- To examine the influence of the introduction of the VSV tag into LPH, the processing, localization, and protease sensitivity of both wtLPH and LPHST were compared in COS-1 cells. Immunoprecipitations using the HBB1/909 antibody directed against pro-LPH and mature LPH resulted in precipitation of high mannose (~215 kDa) and complex glycosylated (~230 kDa) LPH (Fig. 4, first four lanes). No mature form (~160 kDa) could be observed, due to the absence in these cells of the protease that is responsible for the alpha /beta cleavage (1, 2, 8, 18). However, both LPHST and wtLPH were present at the cell surface as shown by immunofluorescence labeling (see below). The results clearly show that introduction of the VSV tag did not affect the transport competence of the mutant.


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Fig. 4.   Immunoprecipitation of wtLPH and LPHST from transfected COS-1 cells using the antibodies HBB1/909, P5D4, and V496. Transfected COS-1 cells were metabolically labeled with Tran35S-label for 1 h, and chased for 0 or 4 h. The aliquots were divided and precipitated with the indicated antibodies. Samples were analyzed by SDS-PAGE followed by fluorography. In the P5D4 lane 0, a faint background band is visible. Neither pro-LPHwt nor pro-LPHST are cleaved.

P5D4, the antibody against the VSV epitope tag, did not recognize wild type pro-LPH but did precipitate the tagged LPH construct LPHST (Fig. 4, middle lanes). The V496 antibody directed against an epitope of LPHalpha precipitated both constructs (Fig. 4, last four lanes). The pro-LPH species precipitated by the V496 antibody corresponds to the mannose-rich precursor as was determined by endo H treatment of the precipitates (not shown).

Trypsin Sensitivity Assay-- To compare sensitivities toward protease treatment of both LPH and LPHST proteins, they were precipitated from transfected COS-1 cells by the HBB1/909 antibody against LPH. The precipitates were treated for several time intervals with trypsin. In Fig. 5 is shown that insertion of the tag did not alter the sensitivity toward trypsin since both proteins show exactly the same pattern. Trypsin treatment results already after 75 s in a complete cleavage of the high mannose and complex forms of wtLPH and LPHST into two bands of around 140 kDa that remain trypsin insensitive for at least 1 h.


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Fig. 5.   Immunoprecipitation of LPH and LPHST from three Caco-2 clones: WT2 and ST54, two clones transfected with wtLPH and LPHST, respectively, and PD10, which expresses LPH endogenously. Confluent Caco-2 clones were metabolically labeled for 1 h, and chased for 0, 4, and 24 h. LPH was immunoprecipitated from lysates using the HBB1/909 monoclonal antibody. Precipitates were treated with endo H (+ lanes) or left untreated (- lanes). Precipitates were analyzed by SDS-PAGE followed by fluorography. The processing pattern of transfected wtLPH and LPHST is essentially the same as the pattern of endogenous LPH. In the ST54 lanes, a persistent, endo H insensitive background band is marked with an asterisk (*). This band is also observed in immunoprecipitations of other proteins (sucrase-isomaltase, DPPIV) from Caco-2 clones, and is also faintly visible in lanes 1 and 7.

After 75 s, a band of about 100 kDa can be observed that gradually disappears after 15 min. In the same time, a band of about 69 kDa appears. Most likely these bands comprise LPHalpha as proposed earlier (1, 2).

Since differently folded molecules are expected to behave differently toward the same protease, in this case trypsin, the results lend strong support to the notion that the folding of LPH and LPHST in COS-1 cells is similar.

Processing and Enzymatic Activity of wtLPH and LPHST in Caco-2-- A Caco-2 clone with an undetectable endogenous LPH expression (TC7) (11) was transfected with the pLPHST and pLPHwt constructs. Stable transfectants were selected with puromycin and screened using immunofluorescence microscopy with antibodies directed against LPHbeta . From each transfection, the clone with the highest expression was chosen for further studies. For the LPHST construct, this clone is called ST54, the wtLPH clone is WT2. As a control, a clone with an endogenous LPH expression (PD10) (11) was used.

Immunoprecipitation of pro-LPH and mature LPH from pulse-chased WT2 cells shows that LPH is synthesized as a 215-kDa single-chain polypeptide (Fig. 6, lanes 1 and 2), which becomes complex glycosylated to 230 kDa and therefore endo H insensitive after 4 h of chase (lanes 3 and 4). At this point, LPHbeta appears as a faint band of 160 kDa. This band is more intense after 24 h of chase when the high mannose form has disappeared (lanes 5 and 6). This processing pattern matches the pattern observed in PD10 cells (lanes 13-18). In intestinal explants, the processing appears to be somewhat faster (2) but is essentially the same.


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Fig. 6.   Trypsin sensitivity assay of LPH and LPHST expressed in COS-1 cells. Transfected COS-1 cells were labeled with Tran35S-label for 4 h. LPH was precipitated using the HBB1/909 antibody. Precipitates were treated with 50 µg/ml trypsin for the indicated time intervals and analyzed by SDS-PAGE followed by fluorography.

Introduction of a VSV tag in LPH did not influence the processing since LPHST was processed in ST54 cells (Fig. 6, lanes 7-12) in a comparable fashion to wild type LPH in both WT2 and PD10 cells. Sometimes a band of about 180 kDa is observed in Fig. 6 (lanes 1, 3, 7, 9, 11, and 13). This is a nonspecific background band that can be observed in other immunoprecipitations as well, like with anti-sucrase-isomaltase antibodies from not only ST54 but from other Caco-2 cells as well (data not shown).

The enzymatic activity of immunoprecipitated lactase from a 165-cm2 culture flask wtLPH or LPHST transfected Caco-2 cells was determined. The lactase precipitated from both cell lines was capable of cleaving 59 and 61 nmol of lactose per minute, respectively. The protein quantities as determined by Coomassie staining of an SDS-PAGE gel with the immunoprecipitates were essentially the same. Therefore we conclude that both constructs display comparable lactase activities and that the introduction of the tag has no influence on this activity.

Immunoprecipitation of LPHalpha -- V496 is an antibody directed against the 12 amino acids following the signal sequence of pro-LPH (2). In previous studies, a 100-kDa band could be observed after immunoprecipitations from intestinal explants using this antibody, which was suspected to be LPHalpha (2). A band of this size was never observed in immunoprecipitation studies from Caco-2 clones in this study. Only the high mannose pro-LPH could be precipitated by the V496 antibody from both Caco-2 WT2 and ST54 cells. No complex glycosylated bands were observed even after a chase period of 24 h (not shown). Apart from this high mannose band, no other specific band was visible. This lower affinity of V496 for complex glycosylated LPH was found in COS-1 cells as well.

P5D4 did not precipitate any specific protein from WT2 cells but precipitated both high-mannose and complex glycosylated form from ST54 cells (not shown). No other specific band could be found. Overall no specific LPHalpha band could be precipitated by the different antibodies and from the different cellular models.

Localization of wtLPH and LPHST in COS-1 Cells-- HBB1/909 is a monoclonal antibody that recognizes pro-LPH and mature LPH (6). In immunofluorescence studies on permeabilized wtLPH and LPHST-transfected COS-1 cells, this antibody labeled the cell surface and an intracellular network, probably the endoplasmic reticulum and the Golgi apparatus (Fig. 7, panel A and D). On nonpermeabilized cells, only the surface was labeled (not shown). The V496 antibody labeled an intracellular network, probably the endoplasmic reticulum and the endoplasmic reticulum-Golgi intermediate compartment (Fig. 7, panel B and E). On nonpermeabilized cells, no labeling could be observed (not shown), indicating that V496 was not able to label LPH at the cell surface. By contrast, P5D4 did not only show labeling of an intracellular network but also surface labeling on permeabilized cells that were transfected with LPHST (Fig. 7, panel F). P5D4 was also able to label the cell surface of nonpermeabilized LPHST cells (not shown). P5D4 but not V496 recognizes complex glycosylated pro-LPHST, indicating that only complex glycosylated LPHST reaches the cell surface, as was already shown by Sterchi et al. (1). Cells transfected with wtLPH were not labeled by P5D4 (Fig. 7, panel C).


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Fig. 7.   Immunofluorescence microscopy of permeabilized COS-1 (panels A-F) and Caco-2 cells (panel G-L), transfected with wtLPH (panels A, B, C, G, H, and I) or LPHST (panels D, E, F, J, K, and L) using antibodies against pro-LPH and mature LPHbeta (HBB1/909), LPHalpha (V496), or the VSV epitope tag (P5D4). Bar, 25 µm.

Localization of wtLPH and LPHST in Caco-2 Cells-- In immunofluorescence studies using HBB1/909 on permeabilized 5 day confluent Caco-2 WT2 and ST54 cells, a patchy labeling pattern was visible on some clusters of cells, whereas others did not show label (Fig. 7, panel G and J). After subcloning using a limiting dilution protocol, the same distribution was observed (not shown). A comparable mosaic expression has been described for in vivo expression of LPH and for other transfections in Caco-2 cells before (11, 19). Labeling of nonpermeabilized cells also showed a patchy labeling pattern, indicating that mainly the cell surface was labeled (Fig. 8, panel A and D). This surface was shown to be the apical membrane by confocal microscopy (Fig. 9).


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Fig. 8.   Immunofluorescence microscopy of nonpermeabilized Caco-2 WT2 (A and B) and ST54 (C and D) cells labeled with HBB1/909 (A and C) or P5D4 (B and D). Bar, 25 µm.


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Fig. 9.   Confocal microscopy on permeabilized Caco-2 LPHST cells showed that only the apical surface of the cells was labeled. LPH was labeled with HBB1/909 and rabbit anti-mouse-fluorescein isothiocyanate, and the nuclei were stained using propidium iodide. On the line across panel A, a vertical section was made that is represented in panel B. Bar, 25 µm.

Immunoelectron microscopy studies on WT2 and ST54 cells confirmed the immunofluorescence data. Some cells did not have any label, whereas others were clearly labeled the brush border (Fig. 10). At the basolateral membrane (Fig. 10, arrow), no labeling was observed. Some gold particles could be found in the endoplasmic reticulum. The labeling pattern of WT2 (Fig. 10, panel A) and ST54 (panel B) was essentially the same.


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Fig. 10.   Electron microscopy on Caco-2 wtLPH (A) and LPHST (B) cells shows immunogold staining at the brush border (BB). No labeling could be detected at the basolateral membrane (arrows) Bar, 200 nm.

Despite the fact that V496 labeled some intracellular structures in transfected COS-1 cells in immunolocalization studies, no labeling could be found under the same conditions in transfected Caco-2 cells (Fig. 7, panels H and K). This is a consequence of the differences in the steady state in COS-1 and Caco-2 cells. In transiently transfected COS-1 cells, the protein synthesis level is much higher then in stably transfected Caco-2. Therefore relatively more LPH in COS-1 cells is present in its high mannose glycosylated form, and recognized by V496. P5D4 against the VSV tag was able to show a faint label at the surface of a few (less then 0.1%) Caco-2 cells expressing tagged LPH (Fig. 7, panel L; Fig. 8, panel D). This surface staining is most likely labeling of complex glycosylated pro-LPH, which could be precipitated in surface immunoprecipitation experiments on Caco-2 ST54 and WT2 cells using the HBB1/909 antibody (Fig. 11). From the apical membrane, LPHbeta and complex glycosylated pro-LPHST could be precipitated (lane 1). From the basolateral membrane, some pro-LPHST but not LPHbeta could be precipitated (lane 2). Expression of pro-LPH at the cell surface has been found before in biopsy samples (20). In transfected Madin-Darby canine kidney cells, it was found at both membrane domains as well (21, 22). The ultimate fate of pro-LPH on the basolateral membrane, degradation, or transport to the brush border needs further analysis.


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Fig. 11.   Surface immunoprecipitation of Caco-2 ST54 cells. Confluent Caco-2 ST54 cells on filter were metabolically labeled for 24 h. At 4 °C, HBB1/909 was added to the basolateral or the apical compartment of the filter. Cells were lysed, and the antibody-bound LPH was precipitated. The immunoprecipitates were analyzed on SDS-PAGE followed by fluorography. At the apical membrane (Ap), complex glycosylated pro-LPH can be found as well as LPHbeta (mature). At the basolateral side (Bl), only complex glycosylated pro-LPH is present. WT2 cells showed essentially the same pattern (not shown).

Concluding Remarks-- Many proteins undergo proteolytic processing after translation. Among these proteins are lysosomal (23), secretory (24, 25), and some plasma membrane proteins. Most disaccharidases are plasma membrane proteins that are cleaved into their subunits (26). LPH is an example of a disaccharidase that undergoes proteolytic cleavage. Unlike other examples LPH has a C-terminal instead of a N-terminal transmembrane region, and both its active sites are located on one cleavage product. Furthermore, it consists of four homologous regions instead of two, which are probably derived from a double gene duplication (20). Two of these regions, III and IV, contain the active sites and are both localized on the same cleavage product, LPHbeta , which is expressed on the brush-border membrane. The second cleavage product, LPHalpha , possesses the other two homologous regions, I and II, but does not show any activity toward disaccharides (2, 4). Two separate studies have reported a role for LPHalpha as an intramolecular chaperone (2, 5). An additional function could be hypothesized because of the internal homologies and because LPHalpha is relatively large (714 residues). Therefore we developed a model in which we could study LPHalpha in more detail. This model consists of Caco-2 cells expressing wild type or modified LPH containing a VSV epitope tag in its LPHalpha domain for additional recognition possibilities. Immunoprecipitation studies from these cells using both an antibody against LPHalpha and an antibody against the LPHalpha -inserted tag did not result in a specific profragment band. Furthermore, localization studies in Caco-2 cells did not result in specific labeling in any LPH-positive cell. We suggest that in Caco-2 cells LPHalpha is degraded soon after cleavage since no intra- or extracellular accumulation could be observed. Our results do not ascertain whether degradation of LPHalpha occurs on its way to the brush border or at the brush border itself.

    FOOTNOTES

* This work has been supported by Grant 902-22-088 of the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (Medische Wetenschappen) (to J. O.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

1 The abbreviations used are: LPH, lactase-phlorizin hydrolase; VSV, vesicular stomatitis virus; LPHST, LPH tagged with a VSV epitope tag in its alpha  region; wtLPH, wild type LPH; endo H, endo-N-acetylglucosaminidase H; endo F/GF, endo-N-acetylglucosaminidase F/glycopeptidase F; PAGE, polyacrylamide gel electrophoresis.

    REFERENCES
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Abstract
Introduction
Procedures
Results & Discussion
References

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