From the Cellular Pharmacology Laboratories,
Department of Ophthalmology, School of Medicine, University of
California, San Francisco, California 94143-0730 and the ¶ Mayo
Clinic, Rochester, Minnesota 55905
![]() |
ABSTRACT |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Expression of the trabecular meshwork inducible glucocorticoid response (TIGR) gene progressively increases from barely detectable levels to greater than 2% of total cellular mRNA over 10 days exposure of trabecular meshwork (TM) cells to dexamethasone. Cycloheximide blocked most of the TIGR mRNA induction, suggesting a requirement for ongoing protein synthesis. The genomic structure of TIGR (~20 kilobases) consists of 3 exons, and a 5-kilobase promoter region that contains 13 predicted hormone response elements, including several glucocorticoid regulatory elements, and other potentially important regulatory motifs. TIGR cDNA encodes an olfactomedin-related glycoprotein of 504 amino acids with motifs for N- and O-linked glycosylation, glycosaminoglycan initiation, hyaluronic acid binding, and leucine zippers. Recombinant TIGR (rTIGR) showed oligomerization and specific binding to TM cells. Anti-rTIGR antibody detected multiple translational/post-translational forms of TIGR produced by the cells (including secreted 66 kDa/55 kDa glycoproteins/proteins in the media and 55 kDa cellular proteins), whereas Northern blot showed a single mRNA species. The findings suggest potential mechanisms by which TIGR could obstruct the aqueous humor fluid flow and participate in the pathogenesis of glaucoma.
![]() |
INTRODUCTION |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
The trabecular meshwork inducible glucocorticoid response (TIGR)1 protein, which has significant homology in its C-terminal domain with olfactomedins, was initially cloned in our laboratories as a candidate gene for glaucoma using differential library screening in a trabecular meshwork cell culture model (1, 2). Mutations were recently found in this gene that co-segregated with both juvenile and adult forms of the disease (3).
Glaucoma is a major cause of blindness, with its most prevalent form thought to involve the specialized endothelial cells lining the outflow pathway of the eye, termed the trabecular meshwork (TM) (4, 5). The synthesis and/or degradation of a variety of extracellular molecules in the meshwork are thought to be regulated by the TM cells, and alterations in the type or amount of connective tissue elements have been postulated to explain the increased outflow resistance seen in glaucoma cases (6). However, an understanding of the biochemical changes that actually contribute to this process has remained elusive.
Previously, we described a highly expressed protein and related glycoprotein (55 and 66 kDa, respectively) found in the media of TM cell culture, but not in other cell types examined, after a prolonged exposure to dexamethasone (DEX) (2). We used this observation to define a cell culture model for "steroid-induced glaucoma" and elevated intraocular pressure due to corticosteroids. The extracellular induced proteins appeared as reasonable candidates for being involved in steroid glaucoma since the time course and dose response of their induction mimicked the intraocular pressure elevation and increased outflow resistance seen in patients receiving glucocorticoid (GC) therapy (7, 8).
Coincident with our research, there was substantial interest in the glaucoma GLC1A locus (9-11) which was mapped on the basis of genetic linkage studies in patients with juvenile glaucoma, a relatively rare form of the disease. The identification of defects in the TIGR gene in glaucoma that map to the glaucoma GLC1A locus (3) has provided a strong impetus for research into the mechanisms by which the TIGR gene might be involved in outflow obstruction and glaucoma pathogenesis.
The following report describes our characterization of the TIGR gene structure, its induction properties, and potentially important aspects of its extracellular protein/glycoprotein. These findings reinforce the rationale used in our cloning strategy and provide specific leads to understand how TIGR could participate in the obstruction of fluid outflow in the trabecular meshwork.
![]() |
MATERIALS AND METHODS |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Cell Culture and Glucocorticoid Treatments-- TM cells were propagated in tissue culture by techniques described previously (5). Confluent, stable monolayers were exposed to DEX to evaluate GC effects on mRNA expression over the calculated clinically relevant dose and time course (1). TM cells were treated with 500 nM DEX for 10 days for cDNA library construction and 100 nM DEX for 1, 4, 7, and 10 days for evaluating mRNA inductions.
cDNA Selection, Dot Blot Quantitation, and Sequencing
Strategies--
A cDNA library was constructed from total mRNA
of 10 day-DEX-treated TM cells, using Lamda Zap II, and bacterial
strain XL-1 from Stratagene (San Diego, CA) by standard protocols (12). Approximately 4 × 104 phages were used for
differential screening by hybridization of duplicate lifts with
-32P-labeled cDNA made from total mRNA of the
10-day DEX-treated and untreated TM cells as described (13). Clones
differentially expressed were used to make probes and hybridized to
total RNA dot blots from untreated and DEX-treated TM cells at 1, 4, 7, and 10 days. An additional RNA dot blot (of untreated cells) was used
to hybridize to total labeled cellular mRNA for estimation of total
mRNA. The dot blots used four serial dilutions of total RNA (each
being a 3-fold dilution of the original 1 µg total RNA) fixed on
Zeta-probe membranes using HYBRI-DOT (Bio-Rad). Clones that showed
major and progressive induction over the treatment schedule were
selected as candidate cDNA clones for further study. When partial
sequencing and HaeIII digestion showed that these candidates
contained pieces of the same gene, the longest one (~2 kb) was used
for sequencing and for probe preparation.
Genomic Structure Analyses-- The P1-TIGR genomic clone was obtained by screening a PAC library using our TIGR cDNA probe in a contract study with Genome Systems, Inc. (St. Louis, MO). The intronic regions and their sizes were estimated by polymerase chain reaction (PCR) using rTth DNA polymerase, XL (Perkin-Elmer) primers derived from cDNA sequence to amplify sections of the P1-TIGR clone. The results were verified using total genomic DNAs. The exon-intron junctions were established by sequencing the P1-TIGR genomic clone using the nested primers to sequence across the junctions. Due to its large size (~100 kb), only limited sequences were obtained from direct sequencing of the P1-TIGR clone. To facilitate sequencing studies, the clone was digested with EcoRI, HindIII, and PstI and subcloned to pZErO-2.1 vector (Genome Systems, Inc., St. Louis, MO). The subclones that showed positive signals with the TIGR cDNA probe were used. To select clones that have the 5' region of the cDNA for promoter sequencing, a primer near the start codon region (i.e. sk1a TTGGTGAGGCTTCCTCTGG (nt 38-56)), and vector primers (M13 forward and reverse), were used to amplify each of the clones. The sequencing of the cDNA, P1-TIGR genomic clones, and the subclones was achieved using methods designed for the Li-Cor automatic sequencer and/or by standard methods using the Sequenase Kit (U. S. Biochemical Corp.) with [35S]dATP for labeling of the reaction (14). Southern analyses were performed using HindIII, EcoRI, and PstI digestions of total genomic and P1-TIGR DNAs. The start site of the gene was identified by a primer extension study using the sk1a primer and protocol previously described (15). GCG (Genetics Computer Group, Madison, WI) Version 8 was used to carry out homology searches of GenBank, EBI, Swiss-Prot, and EST data bases. Northern analysis using TIGR cDNA was performed for TM cells and tissue blot (CLONTECH).
Cycloheximide Studies by Semiquantitative PCR--
To examine
whether the TIGR induction by DEX is dependent on protein synthesis,
four different incubations of cultured TM cells were examined.
Cycloheximide was used at 10 mg/ml. The first incubation was carried
out with DEX for 24 h; the second incubation was carried with
cycloheximide (CH) for 6 h; the third incubation was carried out
with both DEX and CH for 24 h; and the fourth incubation started
out with 6 h of CH followed by 24 h of DEX. The control used
culture TM cells with no treatment. Gene expression levels of TIGR, MT,
1-ACT, and
-actin were determined by semiquantitative PCR using
serial dilution methods similar to those described by Murphy (16). The
PCR conditions were performed according to the Taq
polymerase protocol of Perkin-Elmer for 30 cycles of amplification. Each includes 94 °C/30 s, 55 °C/30 s, 72 °C/30 s. A
72 °C/10 min extension step was included after the last cycle. PCR
products were stained with ethidium bromide (0.5 mg of dye/ml) after
size fractionation on a 1% agarose gel. Primers used for the study include: 5'-AGCAGTTAGTCCTGAAGGCCC and 3'-CTCCAGAGAGTCTCTCCACC for
TIGR, 5'-ATGGATCCCAACTGCTCC and 3'-TCAGGCGCAGCAGCTGC for MT (17),
5'-CTAATGCTTCTGATGCCTTGG and 3'-CAAATAAAGGGTGATGGGATAG for
1-ACT
(18), and 5'-CCTTCCTGGGCATGGAGTCCTG and 3'-GAGCAATGATCTTGATCTTC for
-actin (19).
Baculovirus Expression, Protein Purification, and Amino Acid Sequencing-- Following the ligation of TIGR cDNA to the PVL1393 vector, the construct was transferred to Sf9 cells. After 96 h of infection, the media and cells were collected for protein studies (20). The expressed TIGR proteins, which were aggregated, became soluble using 0.1% SDS and 1% Triton X-100. The removal of SDS was achieved by dialyses against sodium phosphate buffer, pH 7, and by use of an ion retardation (AG11-A8) column. The recombinant proteins were then partially purified by a Sephadex G-150 column. SDS-PAGE gel showed two protein bands in the 55-kDa range, referred to here as doublet 55-kDa proteins (a) and (b). For amino acid sequencing, the proteins were transferred to membranes, cut out separately, eluted, and precipitated for sequencing by an Applied Biosciences 470A sequencer with on-line 1208 PTH.
Antibody Synthesis, Western Blot, and Immunoprecipitation Assays
of Cultured TM Media, Cells, and Perfusates of Organ Culture
Eyes--
For antibody production, approximately 300 mg of partially
purified rTIGR was injected intradermally in rabbits. The rabbits received four booster intradermal injections with 200 mg each for 4 weeks before the sera were collected. For Western blots of the
perfusate, TIGR antibody binding was measured by the ECL chemiluminescence detection method (Amersham, UK) according to the
manufacturer instructions. In the immunoprecipitation studies, media
from DEX-treated TM cells or perfusate from eye organ culture were
diluted in radioimmune precipitation buffer (150 mM NaCl, 5 mM EDTA, 50 mM sodium fluoride, 10 mM sodium phosphate, 10 mM -glycerophosphate, 0.05% sodium deoxycholate, 1% Nonidet P-40, and
1 mM dithiothreitol at pH 7.4) for incubation with the
anti-rTIGR antibody at 4 °C overnight. The immune complex was
isolated on protein A-Sepharose and used for SDS-PAGE gel
electrophoresis, followed by autoradiography to identify
immunoprecipitated materials.
Chemical Cross-linking Assays and G-150 Gel Filtration-- Using a chemical cross-linking procedure, diluted TIGR (purified 55-kDa doublet bands only) or conditioned media of DEX-treated TM cells were incubated with 0.05% glutaraldehyde at 20 °C for 2 h (21). The treated samples were analyzed by SDS-PAGE, and the proteins were detected by anti-rTIGR antibody in Western blots.
Scatchard Analysis of Cell Binding-- Recombinant TIGR protein expressed from baculovirus Sf9 cells was metabolically labeled with [35S]methionine (10 mCi/mmol). Specific binding of rTIGR was examined by using labeled rTIGR in the presence and absence of 100-fold excess non-labeled rTIGR in confluent monolayers of cultured TM cells at 37 °C. Eight serially diluted samples were used for Scatchard analysis (22) using 3 h of incubation (as determined by equilibrium studies). The apparent binding constants were calculated based on the observed slopes.
![]() |
RESULTS |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Candidate Clone Selection and Induction Characteristics--
Nine
clones were confirmed to have progressive GC-induction characteristics
when they were used as probes and hybridized to total RNA of 1, 4, 7, and 10 day-DEX-treated TM cells. These clones were found to have
overlapping parts of the same sequence. The longest clone (2-kb insert)
was used for sequencing of TIGR and synthesis of its recombinant
protein after confirming that it had a full-length ORF. Other
GC-regulated clones that were found included 1-ACT (3 clones, minor
induction) and IGF-BP4 (8 clones, major reduction). Fig.
1a demonstrates the
progressive induction of TIGR mRNA in a separate experiment. The
results showed that the GC induction of TIGR matched the selection
criteria we chose based on the steroid-induced glaucoma model (1, 2)
and the clinical response (7, 8).
|
|
TIGR Is a Leucine Zipper Glycoprotein with Homology to Olfactomedin-- Structural analyses of the TIGR cDNA sequence demonstrated it to be an extracellular molecule with homology to olfactomedin (23) (see Fig. 7a). The proposed ORF for TIGR has two possible start (ATG) sites adjacent to one another. The TIGR gene codes for glycosylation and extracellular binding sites, including an N-glycosylation site, Asn-Glu-Ser (at aa 57-59) (24), seven potential O-glycosylation sites, including Ser-Pro, Pro-Ser, Thr-Xaa-Xaa-Pro, Ser-Xaa-Xaa-Xaa-Pro throughout the molecule (24). It also has a consensus HA binding sequence Arg-Arg-Gly-Gln-Cys-Pro-Ser-Thre-Arg (at aa 181-189) that resembles the consensus motif B(X7)B, in which B is a basic aa and X is any aa (25). The cDNA also contains the GAG initiation sites Asp-Gln-Ser-Gly (at aa 42-45) and Ser-Gly-Glu-Gly (at aa 238-241) sequences that match consensus motifs Asp-Xss-Ser-Gly and Ser-Gly-Xaa-Gly described for proteoglycans (26, 27). Other important features of the molecule include clusters of two and seven leucine zippers and an olfactomedin homology domain of 178 aa at the carboxyl terminus that is considered in greater detail later. General features of the cDNA sequence include a signal sequence that is leucine-rich following the second ATG site, and two polyadenylation signal sequences in the 3'-untranslated region. The amino acid sequence proposed for TIGR and the predicted structural motifs are presented in Fig. 3.
|
Southern Analyses of Total Genomic and P1-TIGR Cloned DNAs-- EcoRI, HindIII, BamHI, PstI, and BglII restriction digestion showed identical patterns for both genomic and cloned DNAs indicating a single copy gene (data not shown). Northern analysis of total RNA isolated from 7 day-DEX-treated TM cells showed a single band at approximately 2.5 kb (Fig. 1b). Heart and skeletal muscle (but not brain, placenta, lung, liver, and kidney) showed a positive signal when commercially available multiple tissues (CLONTECH) were hybridized with the TIGR cDNA probe (Fig. 1b).
TIGR Codes for Multiple Translational and Post-translational Forms-- Immunoprecipitation assays using antibody against rTIGR were performed to evaluate secreted forms of TIGR in the media of DEX-treated TM cells labeled with [35S]methionine. As expected, the 66-kDa glycoprotein was found as the major extracellular form, and doublet proteins near 55-kDa range were found in smaller amounts (Fig. 4A). These precipitated proteins support the idea that glycosylated and non-glycosylated forms of TIGR are expressed extracellularly after prolonged DEX treatment of TM cells. Amino terminal sequencing of the 55-kDa doublet forms of the rTIGR protein (Fig. 4C) confirmed our proposed ORF for its cDNA sequence. The MRFFCA sequence obtained for the higher molecular mass doublet protein (a) confirmed the utilization of the first ATG. The RTAGL sequence obtained for the lower molecular mass doublet protein (b) agreed with the predicted cleavage site Ala-Arg (at aa 32-33) (28) for the proposed ORF. Based on the protein sequencing and antibody results, TIGR cDNA appears to code for the doublet 55 kDa (a) and (b) proteins of 504 and 472 aa, respectively. The finding that both the 66-kDa glycoprotein and 55-kDa doublet proteins are precipitated by the anti-rTIGR antibody indicates that the 66-kDa could be the glycosylated form of one (or both) forms of the 55-kDa protein. The putative motifs for secretion and glycosylation identified for the TIGR cDNA sequence also go along with this view. Our prior biochemical studies of these proteins using tunicamycin had suggested that N-glycosylation might be an important component of the 66-kDa glycosylated form of TIGR (29). The 55-kDa proteins could also be found in the DEX-treated TM cells, in a much lesser amount. The multiple forms of TIGR appear to be the result of translational and post-translational events of TIGR gene expression since only a single gene copy and transcript were found for TIGR. Proposed structures for the doublet 55-kDa (a) and (b) and the 66-kDa glycosylated (c) forms of TIGR found in the media are described graphically in Fig. 5 (iii).
|
|
TIGR Gene Structure-- Two genomic clones, PAC-59-F4 and PAC-63-B19, were obtained in a screening of approximately 10,000 PAC clones using TIGR cDNA. The clones appeared to be similar to each other based on identical mapping by the HindIII digestion of their DNAs and on a Southern assay using TIGR cDNA probe to show identical banding patterns. The clones were estimated to be about 100 kb and termed P1-TIGR. The subclones (that showed positive signals when hybridized to TIGR cDNA) include six EcoRI clones (namely 4A, 6A, 9B, 10B, 12B, and 2C), five HindIII clones (12C, 1D, 1E, 9E and 12E), and one Pst-I clone (1F). Our mapping results showed clones 12B (7 kb), 1E (6 kb), and 4A, 6A, 6B, 9B, 2C (5 kb) were overlapping and clones 9E, 12E, 1F, (2 kb), 10 B (2.5 kb), and 12C (4 kb) were overlapping. Positions of these subclones are shown in Fig. 5(i). Based on the mapping and sequencing of these subclones, the TIGR gene structure was established. This structure includes three exons interrupted by two introns, plus 5' and 3' untranslated regions regions (Fig. 5(ii)). The transcriptional initiation sites include two cytosine residues adjacent to the TATA and CAT boxes.
Due to the possibility that GCs could initiate gene expression from far upstream GRE elements (30, 31), we sequenced a 5-kb region of the P1-TIGR genomic clone to characterize its promoter structure (Fig. 6). Putative sequences found for the TIGR promoter include (a) consensus sequences TATA, CAT boxes, start sites; (b) multiple hormone and cell signaling response elements including seven GREs, three nGREs (four GREs and two nGRE are proximal, within 2.5-kb promoter region), three ERE (estrogen response element), a PRE (progesterone response element), and a TRE (proximal thyroid response element); (c) early and immediate gene response elements including an SRE (serum response element), three AP-1 sites and an AP-2 site, and an ICS (interferon consensus sequence); (d) elements that could relate to oxidative damage, DNA damage, shear stress, and heat shock responses, including one NF-
|
Homology-- Using the Blast search, the best fit of TIGR cDNA sequence was found for mucus olfactomedin family members at the carboxyl terminus. Its leucine zipper domain has homology to various known leucine zipper proteins. The alignment presented in Fig. 7a shows the TIGR homology to olfactomedin of bullfrogs (23, 34), the Z domain of a neuron-specific olfactomedin-related glycoprotein from rat brain (35), and an EST sequence from human brain (36). These domains share very similar aa positions to one another, indicating a closely related family gene (with an exception being the truncated human clone in which the position with respect to its full-length sequence has not been established). A consensus motif for TIGR and olfactomedins is also proposed. Of particular interest is the cysteine residue 433 within the most conserved region of 11 aa found for species. This cysteine residue was thought to be involved in the protein oligomerization by disulfide-linked polymer formation in other olfactomedins.
|
High Molecular Mass Complex Formation of TIGR Protein-- Evidence suggesting that TIGR could oligomerize was found in various assays. In G-150 gel filtration studies, we found that TIGR protein existed in a high molecular mass form above 200 kDa (Fig. 8a), while its monomer (55 kDa) could be obtained when placed in heated SDS under reduced conditions. A cross-linking study was used to further evaluate the possible roles of TIGR's leucine zipper domains for oligomerization. Western analysis of the cross-linked products for diluted rTIGR or conditioned media of DEX-treated TM cells demonstrated that TIGR could exist as a dimer or oligomer (seen as 110 and 200 kDa in equal amounts in Fig. 8b). It is possible that both leucine zippers and the conserved cysteine 433 residue contribute to this high molecular mass formation as discussed later.
|
Characterization of TIGR Binding Affinities to TM Cells--
Fig.
9 shows evidence for two sites of rTIGR
protein binding to TM cells. A saturable site with high affinity
(kd = 4.3 × 109 M)
and a non-saturable site with lower affinity (kd = 2.3 × 10
8 M) were observed. The high
affinity site in TM cells was not observed in similar analyses
conducted using fibroblasts or Matrigel, which did show the low binding
affinity site (data not shown). The findings were verified by two
additional studies. The high affinity site suggests a possible
ligand-receptor type for TIGR-TM cell interactions. It is likely that
this interaction does not involve intergrin receptor since no RGD motif
(42) was identified for TIGR.
|
DEX-induced Organ Culture Expression of TIGR-- DEX produced a progressive increase of TIGR expression in the trabecular meshwork organ culture system as shown in Fig. 4B. This finding demonstrated that the GC-induction of TIGR is not a cell culture artifact. The 55-kDa doublet proteins were detected by Western assay in the perfusate from the organ culture eye, using the rTIGR polyclonal antibody. The 66-kDa form of TIGR might be retained in the meshwork due to predicted interactions with other extracellular matrix molecules. An in vivo role for TIGR (and its potential effect in obstructing outflow) is reinforced by recent findings of increased TIGR gene products in DEX-treated organ culture and glaucoma eyes (43).
![]() |
DISCUSSION |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
In this paper, we have shown that the induction of the TIGR gene expression in TM cells by prolonged DEX treatment results in high levels of new extracellular proteins. We have presented evidence that the TIGR gene products interact with TM cells and are capable of forming oligomeric complexes. The promoter regulatory elements and protein synthesis requirements found for TIGR may be related to the unusual, prolonged time course of the protein's induction. Evaluations of these data support the original model that led us to the cloning of TIGR as a glaucoma gene and also provide new insights into the biochemical and cellular mechanisms that could explain the molecule's effects.
The cycloheximide study, which demonstrated a need for protein synthesis for the induction of TIGR, suggested a requirement for new or continuous production of transcription factors. The finding may also help to explain the progressive nature of the TIGR induction which is distinct from more common GC-inductions (such as MT and alpha-1 ACT shown here) that reach maximal levels by 24 h (44).
The high copy number of GREs, nGREs, and other hormone response elements found throughout the promoter region suggests the importance of Cis-acting elements for the observed progressive GC-induction of TIGR. Our recent studies2 have identified a sequence alteration adjacent to one putative GRE composite site (45) that co-segregated with glaucoma in a chronic open-angle glaucoma pedigree. The role of this or other potential promoter mutations in glaucoma pathogenesis is currently being explored. The variety of cellular regulators that might interact with the TIGR gene's promoter based on the motifs present suggest potential physiological as well as stress-response roles for the TIGR protein/glycoprotein.
A large amount of the 66-kDa glycoprotein was found in the media of DEX-treated TM cultures, with some of the 55-kDa form detected in both media and cells. The ability of anti-rTIGR antibody to precipitate both forms agrees with our previous suggestion that the 66-kDa form could be due to glycosylation of the 55-kDa protein (1, 2). The predicted N- and O-glycosylation sites identified in the TIGR sequence also support this possibility. The potential GAG initiation and GAG binding sites could also be of relevance to the molecule's extracellular interactions. GAGs, including HA, are distributed throughout the TM, and HA has been of substantial interest concerning its influence on fluid dynamics in the meshwork (46).
The sequence of TIGR cDNA, including two ATG sites near the amino terminus, provides interesting clues to the multiple forms of TIGR expression in TM cells. Although the ATG sites do not have the consensus Kozak sequence (47), criteria for a consensus signal sequence (i.e. an 18-20 aa leucine-rich sequence from its putative cleavage site Arg-Ala) (28) suggests that the 55 kDa (b) form (472 aa) could have been made from a protein in which the second ATG site served as the point of initiation of translation. The presence of this mature form (b) in the cells, and some of the larger form (a) in the media without its signal sequence being cleaved, indicates that the protein trafficking studies of TIGR gene products in the TM cells would be useful.
The significant homology of TIGR to the mucus glycoprotein olfactomedin could be relevant to an understanding of TIGR's postulated extracellular interactions. Importantly, this domain is the region in which several structural mutations have been described that have been linked with glaucoma phenotypes (3, 48-50). The observation that this domain is highly conserved when the olfactomedin genes of fish, frog, rat, mouse, and human are compared (51) supports its functional significance. The olfactomedin domain may play a distinct role compared with other domains of the molecule. This is supported by studies of the olfactomedin-related brain glycoprotein gene which produced four different molecules (AMY, BMY, AMZ, and BMZ), two of which contain an olfactomedin-like domain within their Z regions (35). Oligomerization is known to be a characteristic of olfactomedins, but not much is known about this function. In the case of TIGR, oligomerization could be a particular important feature in the obstruction of the trabecular meshwork.
On a mechanistic level, both the apparent ligand-receptor interaction of TIGR with the TM cells and the oligomerization of TIGR could have an important impact on the outflow. Polymerization of the molecule is also suggested by the elution of rTIGR above 150 kDa during isolation by Sephadex G-150. It is possible that TIGR dimers or polymers are linked to form a higher molecular mass structure via a cysteine-cysteine formation similar to that predicted in olfactomedins (23). Mutations affecting this domain might alter TIGR oligomerization in a way that could increase its cell surface and/or matrix interactions and contribute to intraocular pressure elevation. Independently, Raymond et al. (50)3 postulated a role for TIGR oligomerization to help explain their genetic findings, in which homozygotes with TIGR mutations were normal while heterozygotes showed the disease for some glaucoma family members.
While most of our findings and prior concepts of outflow resistance in the eye direct attention at the extracellular function of TIGR, we cannot exclude intracellular interactions with cytoskeleton or other cellular structures as playing a role. The report of Kobuta et al. (37) that described a myosin-like protein (called myocilin or MYOC, GenBank accession no. D88214, which has cDNA sequence that differs from that of TIGR by only a 2-base pair GA insertion at nt 104-105) supports a structural role for the 55-kDa gene products in the cilium of photoreceptor cells. The authors emphasized that myocilin could have myosin-related properties, due to the similarity of the leucine zipper domain for myocilin and myosin. This idea was also brought forward by Coca-Prados et al. (52) in a report showing a high expression of TIGR in iris muscle. However, as we pointed out in Fig. 7b, the leucine zipper domain also has substantial homology with other proteins. This raises the question of whether TIGR's biological properties should be linked to myosin on the basis of the leucine zippers. Although we have not evaluated whether TIGR is glycosylated or exists extracellularly in muscle or photoreceptors, our cell culture findings support the idea that TIGR's extracellular forms could be TM cell-specific. If confirmed by further evaluations, the cell type-specific expression of the TIGR gene could be of importance to understanding its roles in glaucoma.
In summary, our findings have shown that TIGR is primarily expressed as a major extracellular glycoprotein/protein after sustained GC exposure. Characterization of the gene and its products has provided clues as to the mechanisms by which the TIGR could produce biochemical changes that result in physiologic and pathogenic effects.
![]() |
ACKNOWLEDGEMENTS |
---|
We thank Drs. Julia E. Richards and Robert R. H. Anholt for helpful discussions relating to glaucoma genetics and olfactomedin research, respectively. We appreciate the help of Dr. Larry Takemoto for the synthesis of the anti-rTIGR antibody.
![]() |
FOOTNOTES |
---|
* This work was supported by National Institutes of Health Grants EY08905 (to T. N.), EY02477 (to J. P.), EY07065 (to D. J.), and EY02162 (University of California, San Francisco, Ophthalmology Core grant), The Glaucoma Research Foundation, That Man May See, Inc., and InSite Vision, Inc.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) U85257, AF007562, AF007563, AF007564, AF007565, AF007566, and AF012654.
§ To whom correspondence should be addressed: Tel.: 415-476-5633 (T. N.), 415-476-2827 (J. R. P.); Fax: 415-502-2926 (T. N.), 415-476-0336 (J. R. P.); E-mail: thai{at}cgl.ucsf.edu.
1 The abbreviations used are: TIGR, trabecular meshwork inducible glucocorticoid response; TM, trabecular meshwork; rTIGR, recombinant TIGR; GC, glucocorticoid; GRE, glucocorticoid response element; nGRE, negative glucocorticoid response element; CH, cycloheximide; MT, metallothionein; ACT, antichymotrypsin; DEX, dexamethasone; PAC, P1-derived artificial chromosomes; EST, expressed sequence tag; nt, nucleotide(s); aa, amino acid(s); kb, kilobase(s); PCR, polymerase chain reaction; PAGE, polyacrylamide gel electrophoresis; ORF, open reading frame.
2 Nguyen et al., unpublished data.
3 Raymond, V., personal communication.
![]() |
REFERENCES |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|