Inhibitory Specificity of the Anti-inflammatory Myxoma Virus Serpin, SERP-1*

Piers NashDagger §, Adrian Whittyparallel , Jason Handwerkerparallel , Joanne MacenDagger , and Grant McFadden§parallel **

From the Dagger  Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada, parallel  Protein Engineering Department, Biogen Inc., Cambridge, Massachusetts 02142, and the § Department of Microbiology and Immunology, University of Western Ontario and The J. P. Robarts Research Institute, London, Ontario N6G 2V4, Canada

    ABSTRACT
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Abstract
Introduction
Procedures
Results
Discussion
References

SERP-1 is a myxoma virus-encoded serpin, secreted from infected cells, that is required for virulence and has anti-inflammatory activity. We report that purified recombinant SERP-1 forms SDS-stable complexes with urokinase-type plasminogen activator (uPA), tissue-type plasminogen activator (tPA), plasmin, thrombin, and factor Xa. N-terminal sequencing confirmed Arg319-Asn320 as the site of reaction. Mutation of these residues to Ala-Ala abolished inhibitory activity but had no effect on the specific cleavage at Thr315-Leu316 seen with elastase and with cathepsin G. Kinetic analysis of the reactions with uPA, tPA, plasmin, thrombin, Xa, and C1s showed second-order rate constants to vary over 3 logs, from kinh = 3 × 105 M-1 s-1 with thrombin to ~600 M-1 s-1 with C1s, while steady-state inhibition constants ranged from KI = 10 pM with thrombin to ~100 nM with C1s. Stoichiometries of inhibition varied between SI = 1.4 ± 0.1 for uPA to SI = 13 ± 3 for thrombin. Analysis of the variations in inhibition kinetics shows that when serpins act at low concentrations, comparable with the target protease or with KI (as appears likely for SERP-1 in vivo), inhibitory specificity becomes less dominated by kinh and is increasingly dependent on partitioning within the branched reaction mechanism and on the lifetime of the inhibited complex.

    INTRODUCTION
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Abstract
Introduction
Procedures
Results
Discussion
References

Serpins comprise a conserved superfamily of single-chain proteins that is widely distributed in nature. Almost all serpin family members have activity as inhibitors of serine proteinases, and serpins have been shown to serve in the regulation of a variety of proteinase-mediated processes including extracellular matrix remodeling, modulation of inflammatory responses, fibrinolysis, complement activation, and blood coagulation (1). At least 90 naturally occurring serpin mutations have been associated with human disease (2). Given the critical role that proteinases and serpins play in the maintenance of homeostasis, it is not surprising that certain parasitic organisms use serpins to disrupt host processes that would otherwise be antagonistic to the parasite's survival. Several filarial parasites have been reported to express serpins (3-5), as have members of the poxvirus family of large DNA viruses (6). Myxoma virus, a leporipoxvirus, expresses SERP-1, the only known virus-encoded serpin that is secreted from infected cells (7, 8). Infection with myxoma virus produces a rapidly lethal disease state in European rabbits (Oryctolagus cuniculus) known as myxomatosis (9, 10), a characteristic feature of which is the specific down-regulation of host inflammatory and immune responses (11). Myxoma virus causes severe immune dysregulation, which disrupts the ability of the infected host to combat the infection; morbidity is generally the result of supervening Gram-negative infections, since the immunocompromised host is unable to counteract even routine bacterial assault. No single viral protein appears sufficient to cause this degree of immunodysfunction; rather, it is through the combined action of an array of secreted and intracellular factors that the virus accomplishes this task (12). Interest in SERP-1 arose from its identification as the first virus-encoded serpin to be among these virulence factors. The secretion of SERP-1 from infected cells serves to dampen the in vivo inflammatory response to myxoma virus infection, and gene knockout analysis revealed greater inflammation, more rapid resolution of the infection, and a consequent attenuation of virulence upon infection of immunocompetent host animals (8, 13). This finding suggests that SERP-1 contributes to viral pathogenesis by interacting with and inhibiting host proteins involved in the regulation of inflammation. Purified SERP-1 expressed from recombinant vaccinia virus has been tested in inflammatory models for restenosis following balloon angioplasty (14) and rheumatoid arthritis (15) and in both cases proved to be effective at reducing localized inflammation at very low levels of SERP-1 protein. SERP-1 is the product of a gene located within the terminal inverted repeat region of the myxoma genome (16) and thus is present in two copies (8). The expressed gene product is a secreted, N-glycosylated protein with a predicted molecular mass of 39.9 kDa, although the mature glycoprotein migrates as a diffuse band of 50-55 kDa on SDS-PAGE. A preliminary survey of the inhibitory properties SERP-1 using unfractionated supernatants from myxoma virus-infected cells has been reported by Lomas et al. (17).

Serpins interact with proteinases via a flexible exposed loop, known as the reactive center loop (RCL),1 that is critical to inhibitory function but is also susceptible to cleavage by non-target proteinases (18). The inhibitory specificity of serpins is largely defined by the nature of the residues at the P1-P1' positions (19) that flank the site in the RCL at which the serpin becomes cleaved upon reaction with target proteinases (20, 21). The branched kinetic mechanism in Scheme 1 has been proposed to account for currently available data on the mechanism of serpin action (22, 23). A key feature of this mechanism is that it includes an intermediate, [EI], which can partition to react either through hydrolysis to release free enzyme, E, and cleaved serpin, I', or alternatively by undergoing a major conformational change leading to the formation of a highly stabilized covalent inhibited complex, EI* (23-25). The structural rearrangement that produces EI* is accompanied by insertion of a portion of the cleaved RCL into beta -sheet A of the serpin structure (26) and results in a large increase in stability (27). This substantial conformational rearrangement has been suggested to distort the geometry of key residues in the active site of the proteinase and to thereby decrease the ability of the enzyme to achieve its release from the serpin through enzyme-catalyzed deacylation (28). Dissociation of EI* to give active enzyme plus cleaved serpin is typically extremely slow (29).


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Scheme 1.  

The range of proteinases inhibited under a given set of experimental conditions has been established for many serpins, and in numerous cases the kinetics of interaction with one or more proteinase targets have been investigated. However, relatively little attention has been paid to determining which steps in the mechanism contribute to inhibitory specificity. The relative lack of emphasis on this issue may be due to the fact that many of the earliest and most thoroughly investigated serpins are those found in the blood. These serpins typically circulate at concentrations that are orders of magnitude above their IC50 for interacting with target proteinases, such that specificity is governed almost entirely by relative on-rates. However, serpins such as SERP-1 or inhibitors of caspases and other intracellular targets, may act at much lower concentrations in vivo. Under these conditions, the possibility exists that other steps in the mechanism might influence whether or not a particular proteinase will be subject to effective inhibition by a given serpin.

In this report, we describe the purification to homogeneity of wild type SERP-1 and of a R319A/N320A double mutant in which the predicted P1-P1' residues in the RCL are changed to alanine, expressed using a recombinant vaccinia virus expression system. Both purified wild type SERP-1 and R319A/N320A were tested against a panel of proteinases for their ability to form SDS-resistant complexes or to be cleaved at the RCL. We also describe a detailed kinetic study of the reactions of SERP-1 with a panel of six proteinases that were inhibited by the serpin, using the techniques of slow binding inhibition kinetics and inhibition titrations. We interpret the results of these studies in terms of the contributions that specific steps in the inhibition mechanism make toward controlling the inhibitory specificity of SERP-1.

    EXPERIMENTAL PROCEDURES
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Abstract
Introduction
Procedures
Results
Discussion
References

Materials-- The human enzymes plasmin, thrombin, trypsin, cathepsin G, C1s, tissue-type plasminogen activator (tPA, two-chain form), and urokinase-type plasminogen activator (uPA, low molecular weight form) were obtained from Sigma, as were thermolysin, subtilisin, and bovine chymotrypsin. Human neutrophil elastase (hNE), cathepsin G, and C1s were supplied by Calbiochem. Complement factor D was a generous gift of Fred Taylor of Biogen, Inc. The complement proteins factor B and C3, used for the generation of the alternative pathway C3 convertase, were obtained from Quidel (San Diego, CA). Polyacryalamide gels were from Novex (San Diego, CA). Proteinase substrates Chromozym-TRY, Chromozym-TPA, Chromozym-TH, Chromozym-U, and Chromozym-X were purchased from Boehringer Mannheim, and D-Val-Leu-Lys-p-nitroaniline was obtained from Sigma.

Viruses and Cell Lines-- The complete SERP-1 gene, including the signal sequence, was inserted into the thymidine kinase locus of vaccinia virus strain WR under the control of a strong, synthetic late promoter by homologous recombination as described (8), in order to produce a recombinant virus termed VV-S1. At late times (>8 h) after infection of BGMK cells with VV-S1 virus, SERP-1 accumulated as a stable glycoprotein in the culture supernatant. Expression was confirmed by Western blot analysis using polyclonal rabbit anti-SERP-1 serum. At 20 h postinfection, the supernatant was collected, and SERP-1 was purified as described below.

Preparation of a Reactive Center Mutant of SERP-1-- Reactive center mutant SERP-1, designated R319A/N320A, was produced using the U.S.E. mutagenesis system (Amersham Pharmacia Biotech). SERP-1 in the p22MAP B/H-8 vector (8) was subjected to site-directed mutagenesis using the oligonucleotide 5'-ATCGCCGTGAGGGCGGCCGCGGGGATGAGGGTGAT-3', which corresponds to a sequence change in the codons responsible for the P1 and P1' residues from Arg319-Asn320 to Ala319-Ala320. The resulting mutant was confirmed by sequencing, and a 1344-base pair BamHI/BglII fragment containing the complete open reading frame was then ligated into the BamHI site of pMJ601 (30). This pMJ-S1AA construct allowed insertion of the mutant SERP-1 gene into the thymidine kinase gene of vaccinia virus under the control of a strong, synthetic late promoter using previously described methods (8). Expression of the R319A/N320A protein from the recombinant vaccinia virus, designated VV-S1AA, was confirmed by immunoblotting using rabbit polyclonal anti-SERP-1 antiserum (8).

Purification of Wild Type and Mutant SERP-1-- BGMK cells (2 × 108 cells) were adsorbed with VV-S1 or VV-S1AA at a multiplicity of infection of 1 plaque-forming unit/cell for 2 h at 37 °C in 10 ml of Dulbecco's modified Eagle's medium containing 10% newborn calf serum. The inoculum was removed, and the cells were washed three times with 50 ml of sterile phosphate-buffered saline to remove medium and serum proteins. Serum-free Dulbecco's modified Eagle's medium (15 ml) was added to each bottle, and the infection was allowed to proceed for 20 h at 37 °C. The culture supernatant was collected, spun at 5000 × g to pellet cells and cell debris, and stored at -20 °C. The medium containing secreted viral proteins was concentrated approximately 50-fold using an Amicon pressure cell equipped with a 10-kDa cut-off membrane and was then dialyzed against 25 mM Tris, pH 8.0 at 4 °C using Spectrapore dialysis tubing with a 30-kDa molecular weight cut-off. The dialyzed samples were spun at 14,000 × g to remove precipitates, loaded onto a MonoQ anion exchange column (Amersham Pharmacia Biotech), and eluted with a linear salt gradient (0-300 mM NaCl in 25 mM Tris, pH 8.0). Fractions were analyzed by SDS-PAGE, and those containing SERP-1 were identified by immunoblotting using anti-SERP-1 antiserum (8), pooled, and concentrated to 1 ml using Centriprep 10 concentrators (Amicon). This material was further purified on a Superdex 75 gel filtration column (Amersham Pharmacia Biotech) (150 mM NaCl, 25 mM Tris, pH 8.0). Fractions were collected and analyzed for SERP-1 by SDS-PAGE and visualized by silver staining and immunoblotting using anti-SERP-1 antiserum (8).

Western Blot Analysis of the Products of Reaction of SERP-1 with Proteinases-- SERP-1 (180 nM) was incubated with a slight molar excess of each proteinase for the times indicated in a total reaction volume of 10 µl (100 mM NaCl, 2 mM CaCl2, 0.005% Triton X-100, 100 mM Tris-HCl, pH 7.5). Control experiments established that both SERP-1 and the proteinases are stable under these conditions. Reactions were quenched by boiling for 5 min in SDS-loading buffer containing 100 mM dithiothreitol and 2% SDS. Samples were separated on a 4-20% linear gradient or 10% Tris-glycine SDS-polyacrylamide gels (Novex) using the Laemmli buffer system (31). Proteins were transferred to Hybond C-extra (Amersham Pharmacia Biotech) nitrocellulose by electroblotting; blots were blocked in TBS (150 mM NaCl, 2.5 mM KCl, 25 mM Tric-HCl, pH 7.4) containing 5% (w/v) skimmed milk powder and 0.1% (v/v) Tween 20 for >2 h; and SERP-1 was detected by incubating for 1 h with 0.05% (v/v) polyclonal rabbit anti-SERP-1 antiserum (8) in blocking buffer. After washing with TBS containing 0.1% (v/v) Tween 20, a secondary goat anti-rabbit antibody conjugated to horseradish peroxidase (Bio-Rad) was applied for 1 h. Immunoreactive SERP-1 species were visualized using ECL detection (Amersham Pharmacia Biotech) on Eastman Kodak Co. x-ray detection type film. Reactions that showed complete cleavage of SERP-1 by the proteinase, with no evidence of the formation of a stable SERP-1-enzyme complex, were repeated with shorter incubation times in order to see whether reactions proceeded via a transient inhibited complex. Reactions in which the serpin appeared unchanged at the end of the incubation period were repeated at higher enzyme concentrations and for longer incubation periods.

Cleavage Site Sequence Analysis-- The position at which SERP-1 becomes cleaved upon treatment with thrombin, cathepsin G, or human neutrophil elastase was determined by N-terminal sequencing of the 5-kDa C-terminal cleavage fragment of SERP-1, according to Hewick et al. (32). Briefly, 30 pmol of SERP-1 was digested to completion by thrombin, cathepsin G, or hNE. The C-terminal fragment of SERP-1 was separated by SDS-PAGE and transferred to ProBlott membrane (Applied Biosystems) by semidry eletroblotting (10 mM CAPS, 10% methanol, pH 11). Protein bands were visualized by Coomassie Blue R-250 staining, and the 5-kDa band corresponding to the C-terminal fragment of SERP-1 was excised from the blot, washed in 50% HPLC grade methanol/water, and air-dried. The excised membrane was loaded onto an Applied Biosystems (ABI) model 470A gas phase sequencer, and the resulting phenylthiohydantoin-derivatives were analyzed on-line using an ABI 120A analyzer equipped with a phenylthiohydantoin C18 2.1 × 220-mm column. Data were analyzed using ABI 610A software.

Standardization of Enzyme and SERP-1 Solutions-- Plasmin, thrombin, and uPA were standardized by active site titration according to method A of Jameson et al. (33). Briefly, enzyme was added at a final concentration of 10-60 nM to a stirred quartz cuvette containing 4-methylumbelliferyl p-guanidinobenzoate (MUGB; 0.8-2.0 µM) in a total volume of 2.5 ml (100 mM Tris-Cl, 100 mM NaCl, 2 mM CaCl2 and 0.005% Triton X-100, pH 7.5, 25 °C). The molar concentration of active enzyme was determined from the magnitude of the burst in 4-methylumbelliferone (4-MU) formation upon addition of the enzyme, converted to molar units by reference to a standard curve for 4-MU fluorescence measured under identical conditions. Control experiments showed that the size of the burst was directly proportional to the final concentration of each enzyme. Concentrations of active enzyme in each stock solution were determined from the mean of at least three measurements. Individual measurements differed from the mean by <= 3% for uPA and plasmin and by <= 8% for thrombin. Factor Xa reacted with MUGB too slowly to allow this method to be used, so a concentrated incubation method (method B of Jameson et al. (33)) was used for this enzyme. This involved incubating 15, 30, or 40 µl of a factor Xa stock solution (~5 µM) with 10 µl of aqueous MUGB solution (0.2 mM) in a total reaction volume of 50 µl (final buffer: 80 mM Tris-Cl, 80 mM NaCl, 1.6 mM CaCl2, and 0.004% Triton X-100, pH 7.5, 25 °C). Aliquots (10 µl) were removed at 15-min intervals and diluted to a total volume of 2.5 ml in a quartz fluorescence cuvette (100 mM Tris-Cl, 100 mM NaCl, 2 mM CaCl2, and 0.005% Triton X-100, pH 7.5, 25 °C). The concentration of active factor Xa was determined by fluorometric measurement of the concentration of 4-MU released by reaction with the enzyme, extrapolated to zero reaction time and corrected for the background hydrolysis of MUGB by subtraction of the 4-MU fluorescence seen in a background reaction containing 0.2 mM MUGB but no enzyme. We estimate a precision of ±6% for these measurements. Neither tPA nor C1s reacted suitably with MUGB to allow standardization of these two enzymes with this reagent.

The concentration of purified SERP-1 was determined by absorbance at 280 nm using a molar extinction coefficient of 32,700 M-1 cm-1. This value was measured using mammalian cell-derived recombinant SERP-1 with an identical amino acid sequence.2 The experimentally determined extinction coefficient was within 5% of that calculated from the amino acid sequence according to the method of Gill and von Hippel (34).

Km Determinations for Chromogenic Proteinase Substrates-- Estimates of Km for the reaction of each enzyme with the appropriate chromogenic substrate were determined directly under the conditions used for the slow binding inhibition experiments. Km values for the reactions of uPA with Chromozym-U, tPA with Chromozym-tPA, C1s with Chromozym-TRY, thrombin with Chromozym TH, and factor Xa with Chromozym X were found to be 140 µM, 160 µM, 1.5 mM, 22 µM, and 510 µM, respectively.

Slow Binding Inhibition Kinetics-- Inhibition progress curves were obtained by incubating a limiting concentration of proteinase (5-125 pM) with various concentrations of SERP-1 from 0.125 to 100 nM in the presence of the appropriate chromogenic substrate. In all cases, serpin was present at >= 10-fold excess over proteinase in order to achieve pseudo-first-order conditions with respect to SERP-1, and concentrations of enzyme and substrate were chosen so that <= 10% of the substrate was hydrolyzed over the entire duration of the assay. Reactions were performed at 37 °C in a total volume of 800 µl (100 mM Tris-HCl, 100 mM NaCl, 2 mM CaCl2, 0.005% Triton X-100, pH 7.5) in plastic cuvettes sealed with Parafilm. The time-dependent inhibition of each enzyme by SERP-1 was monitored by following the rate of production of p-nitroaniline at 405 nm at 1- or 5-min intervals for a period of 1000 min, using a CARY 3E spectrophotometer equipped with a six-cell cuvette holder. Each set of six reactions comprised five reactions containing various concentrations of SERP-1 and one control reaction containing enzyme and substrate with no SERP-1. In the [SERP-1] = 0 controls, progress curves were observed to be linear, indicating that the enzyme activity was stable over the course of the reaction. For each enzyme, two to five sets of measurements spanning at least six different concentrations of SERP-1 were used to calculate the kinetic constants. The reactions were allowed to proceed until the steady state velocity of p-nitroaniline formation was attained, and the progress curves for each reaction were then fitted to the integrated rate equation for slow binding inhibition (Equation 1) (35, 36), in which At is the absorbance at 405 nm at time t, due to the evolution of p-nitroaniline; kobs is the apparent first-order rate constant for the inhibition of enzyme by SERP-1; vi and vs are the initial and steady-state velocities for reaction of the chromogenic substrate; and d is the initial absorbance at 405 nm.
A<SUB>t</SUB>=v<SUB>s</SUB>t+<FENCE><FR><NU>v<SUB>i</SUB>−v<SUB>s</SUB></NU><DE>k<SUB><UP>obs</UP></SUB></DE></FR></FENCE><FENCE>1−e<SUP><UP>−</UP>k<SUB><UP>obs</UP></SUB>t</SUP></FENCE>+d (Eq. 1)
Values for vi, vs, and kobs were obtained for the progress curves measured at each SERP-1 concentration using nonlinear regression analysis (DeltaGraph 3.5). Rate constants for the release of active enzyme from the enzyme-SERP-1 inhibited complex, kd, were measured by preincubating proteinase and SERP-1 together at high concentration (10-100 nM) and diluting the resulting inhibited complex 50-fold into a slow binding inhibition assay containing the appropriate chromogenic substrate. Reactivation of the enzyme was monitored by following the absorbance at 405 nm until the new steady state was reached. Progress curves were fitted to Equation 1, for which kobs = kd.

Stoichiometry of Inhibition-- The stoichiometry of inhibition (SI) was measured for the reactions of SERP-1 with uPA, plasmin, thrombin, and Xa using a method similar to that of Patston et al. (37). Accurately known, fixed concentrations of each proteinase were incubated with SERP-1 at various molar ratios in a total reaction volume of 25 or 30 µl (100 mM Tris-HCl, 100 mM NaCl, 2 mM CaCl2, 0.005% Triton X-100, pH 7.5, 37 °C). For each proteinase, a control reaction was included that contained no SERP-1. Enzymes were used at the following concentrations: uPA and plasmin, 40 nM; thrombin, 5 nM; Xa, 16.7 nM. Reactions were incubated for 1 h, except for the slower reaction with Xa, for which a 10-h incubation was required for complete reaction. In all cases, control experiments at longer reaction times were performed to show that reaction was complete and that incubation under these conditions did not cause any significant loss of activity in the enzyme only control. At the end of the incubation period, residual proteinase activity was determined in activity assays containing an appropriate chromogenic substrate. SI values for reaction with each enzyme were determined by plotting residual proteinase activity against the molar ratio of SERP-1 to proteinase and extrapolating the linear portion of the inhibition titration curve to zero residual activity to give the minimum number of equivalents of SERP-1 required to fully inhibit the enzyme. SI values were measured a minimum of three times in at least two independent experiments for each enzyme.

    RESULTS
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Abstract
Introduction
Procedures
Results
Discussion
References

SERP-1 and the RCL mutant R319A/N320A were cloned into a vaccinia virus expression system, expressed and purified from viral supernatant as described under "Experimental Procedures." Fig. 1 shows the purification of SERP-1 protein as monitored by silver-stained SDS-PAGE. Lane 1 shows the crude viral supernatant, lane 2 shows the partially purified material after the MonoQ FPLC step, and lane 3 shows SERP-1 purified through the final gel filtration step to an apparent purity of >95%, as judged by overloaded Coomassie-stained and silver-stained gels and by the observation of a single peak on reversed-phase HPLC (data not shown). Both wild type and mutant SERP-1 migrate on SDS gels as a diffuse band, with an apparent molecular mass of 50-55 kDa due to the presence of N-linked glycosylation (8). The yield of SERP-1 after the two-column purification was ~25 µg/108 BGMK cells infected. A faint Western blot-positive band at 110 kDa, corresponding to a dimer, was sometimes observed by SDS-PAGE, as has been seen for other serpins (8, 17). The presence and intensity of this band varied from gel to gel, even for the same SERP-1 sample. This observation and the fact that the band could be eliminated under conditions of high SDS plus reducing agents (data not shown) suggested that this band was due to formation of a small amount of dimer during SDS-PAGE sample preparation. Gel shift experiments, performed as described below, confirmed that all of the SERP-1 protein was able to react with appropriate target proteinases, indicating that no latent or inactive SERP-1 was present.


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Fig. 1.   Silver-stained SDS-polyacrylamide gel showing stages of purification of the SERP-1 protein. The arrow indicates the position at which SERP-1 migrates, corresponding to an apparent molecular mass of ~55 kDa. Lane 1, crude supernatant from cells infected with VV-S1. Lane 2, pooled fractions recovered from MonoQ FPLC anion exchange chromatography. Lane 3, SERP-1 fraction recovered from gel filtration chromatography on a Superdex 75 column.

Purified SERP-1 Forms SDS-stable Complexes with Plasmin, uPA, tPA, Xa, and Thrombin-- Inhibition of serine proteinases by serpins is accompanied by the formation of an SDS-stable complex that can be seen as a high molecular weight band on SDS gels (38). Lomas et al. (17) have reported that gel-shifted complexes of this type can be observed upon incubation of SERP-1 from unfractionated viral supernatants with tPA, uPA, plasmin, and C1s but not with thrombin, factor Xa, or other proteinases tested. In order to better define the inhibitory specificity of SERP-1, we have repeated and extended their survey by investigating the reactions of purified SERP-1 with an extensive panel of proteinases. While few, if any, of the enzymes tested are plausible candidates for the target of SERP-1 inhibition in vivo, a broad panel of available proteinases was examined in the expectation that the results would help establish what properties in vivo targets for SERP-1 inhibition are likely to possess. In an initial screen, purified SERP-1 (~180 nM) was incubated for 2 h with a slight molar excess of each enzyme (100 mM Tris-HCl, 100 mM NaCl, 2 mM CaCl2, 0.005% Triton X-100, pH 7.5, 37 °C), as described under "Experimental Procedures." The distribution of SERP-1 in the product mixture between native, complexed, and cleaved forms was determined from the migration of the corresponding SERP-1 bands on SDS-PAGE, as detected by Western blotting using a rabbit anti-SERP-1 polyclonal antibody (Fig. 2). In cases where no high molecular weight complex with the proteinase was observed in the initial screen, the experiment was repeated with different incubation times and at different enzyme:SERP-1 ratios to ensure that the existence of a slow forming or transient complex was not being missed. Fig. 2A shows that purified SERP-1 reacts with plasmin (lane 2), uPA (lane 3), tPA (lane 4), and Xa (lane 6) to form SDS-stable complexes that migrate with apparent molecular masses that are consistent with the expected values for their respective complexes with SERP-1 of about 135, 93, 123, and 105 kDa. Most of the inhibited proteinases contain disulfide-linked subunits, which allowed the identity of the higher molecular weight bands to be confirmed by performing electrophoresis under reducing conditions and checking that the apparent molecular weight of the SERP-1-proteinase complexes changed by the expected amounts. In addition to forming an inhibited complex, reaction with these enzymes also produced varying amounts of a species running at about 50 kDa, which is the size expected for SERP-1 that has been cleaved at or near the predicted cleavage site in the RCL. The generation of some amount of cleaved serpin has been frequently found to accompany the inhibition of proteinases by serpins (38, 39), either through the hydrolytic breakdown of the inhibited complex or because of the operation of a branched kinetic mechanism (22). The observation of an inhibited complex with Xa was unexpected, since this enzyme had been reported previously to cleave SERP-1 without forming a stable complex (17). Our result was confirmed by the observation that SERP-1 is able to inhibit Xa in kinetic experiments (see below). Thrombin (lane 5) initially appeared to form exclusively cleaved serpin, migrating at approximately 50 kDa, without the formation of any detectable higher molecular weight complex. However, by reducing the reaction time, we were able to observe the transient formation of a small amount of a SERP-1-thrombin complex, as shown in Fig. 2B. This result was confirmed by the observation that thrombin is inhibited by SERP-1 in kinetic experiments. hNE and cathepsin G also appeared to exclusively form cleaved SERP-1 (Fig. 2A, lanes 8 and 9). However, unlike thrombin, additional tests under varied conditions gave no evidence for any stable complex between SERP-1 and either of these enzymes. The inability of SERP-1 to inhibit cathepsin G was confirmed in kinetic experiments. Reaction with C1s also failed to give evidence for the formation of a stable complex (Fig. 2A, lane 10), although the results of Lomas et al. suggest that SERP-1 does inhibit this enzyme, and we also observed evidence for weak inhibition of C1s by SERP-1 in kinetic experiments. Reaction with trypsin produced two discrete bands, corresponding to the 50-kDa cleaved form seen with the other enzymes together with an additional smaller form of cleaved SERP-1 (Fig. 2A, lane 7); the two fragments appeared rapidly and were stable once formed. Reaction with chymotrypsin also gave two discrete SERP-1 cleavage fragments, although in this case both observed products were substantially smaller than 50 kDa (data not shown). The inability of SERP-1 to inhibit chymotrypsin was confirmed in kinetic experiments (data not shown). No reaction was apparent upon incubation of SERP-1 with kallikrein, factor D, beta -XIIa, or the alternative pathway C3 convertase (Fig. 2A, lanes 11 and 12 and data not shown). Bovine factor beta -XIIa was tested because of a published report of a bovine serpin, believed to target this enzyme, that has an asparagine at the putative P1' position in the RCL and either lysine or arginine at the P1 position (40). This is the only example, other than SERP-1, of a serpin reported to have a P1' asparagine and thus raised the possibility that SERP-1 might target beta -XIIa. Finally, and as expected, proteinases from other structural and mechanistic classes (cathepsin B, cathepsin D, thermolysin, and subtilisin) either failed to react with SERP-1 or caused the complete disappearance of the SERP-1 band, presumably due to its cleavage into small fragments that were not detectable by Western blotting (data not shown). Our results, compared with those of Lomas et al. (17), are summarized in Table I.


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Fig. 2.   A, SERP-1 forms SDS-stable complexes with plasmin, tPA, uPA, and factor Xa. Purified SERP-1 (lane 1) was incubated for 2 h with a slight molar excess of the following proteinases: plasmin (lane 2), uPA (lane 3), tPA (lane 4), thrombin (lane 5), factor Xa (lane 6), human trypsin (lane 7), human neutrophil elastase (lane 8), cathepsin G (lane 9), C1s (lane 10), factor D (lane 11), and factor beta -XIIa (lane 12). The products were separated by nonreducing SDS-PAGE and detected by Western blot using polyclonal anti-SERP-1 antiserum. Uncleaved SERP-1 is visible as an ~55-kDa band in lanes 1, 2, 4, 6, 10, 11, and 12. SERP-1 cleaved in or near the RCL appears as an ~50-kDa band in lanes 3-9. Higher molecular mass bands indicating the formation of SDS-stable complexes between SERP-1 and the proteinase appear in lanes 2-4 and 6. B, SERP-1 forms a short lived SDS-stable complex with thrombin. Purified SERP-1 (180 nM) was incubated with approximately equimolar human thrombin for 2 min (lane 1), 12 min (lane 2), or 60 min (lane 3). A small amount of a high molecular mass complex of 90 kDa, corresponding to the size expected for a complex between thrombin and SERP-1, is seen at 2 and 12 min, but both uncleaved SERP-1 and the complex have disappeared by 60 min, leaving only the band at ~50 kDa that corresponds to SERP-1 cleaved at or near the RCL. C, SERP-1 mutant R319A/N320A does not react with the proteinases that form complexes with wild type SERP-1. Purified R319A/N320A (lane 1) was incubated with an excess of plasmin (lane 2), uPA (lane 3), tPA (lane 4), factor Xa (lane 5), thrombin (lane 6), human neutrophil elastase (lane 7), and cathepsin G (lane 8), and the resulting products were separated by SDS-PAGE and visualized by Western blotting. Intact R319A/N320A appears as an ~55-kDa band seen in lanes 1-6, while cleavage at or near the RCL yields a product of ~50 kDa seen in lanes 7 and 8.

                              
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Table I
Summary of the interactions of wild type and R319A/N320A mutant SERP-1 with various proteinases as determined by SDS-PAGE and Western blot analysis
Purified SERP-1 (180 nM) was incubated at 37 °C for 2 h with a slight molar excess of each proteinase (100 mM Tris-HCl, 100 mM NaCl, 2 mM CaCl2, 0.005% Triton X-100, pH 7.5). The products were separated by SDS-PAGE and detected by Western blot using polyclonal anti-SERP-1 antiserum (see Fig. 2). pPE, porcine pancreatic elastase. I indicates the observation of a high molecular weight band, relative to that for unreacted SERP-1, indicating the formation of an SDS-stable inhibited complex between SERP-1 and the enzyme. The observation of such a complex was invariably accompanied by the formation of some amount of a lower molecular weight species corresponding to SERP-1 that has been proteolytically cleaved in or near the RCL (see Fig. 2). S indicates that only lower molecular weight species and unreacted SERP-1 were observed in the product mixture, indicating that SERP-1 was proteolytically cleaved without the concomitant formation of a detectable high molecular weight complex. N indicates that incubation with the proteinase resulted in no apparent change in the SERP-1 band, even when retested at higher concentrations of enzyme or for longer incubation times. Cases in which the interaction was not tested are denoted ND.

Identity and Functional Importance of P1-P1' Residues in SERP-1-- The location of the P1-P1' cleavage site in the RCL was confirmed by sequencing the N terminus of the 5-kDa SERP-1 cleavage fragment released upon reaction with thrombin followed by denaturation of the reaction products. A sequence of NALTAIVANK was determined, which corresponds to residues 320-329 of the SERP-1 sequence and thus confirms that reaction with thrombin occurs at the bond between Arg319 and Asn320. In order to explore the functional importance of these residues for SERP-1 inhibitory activity, a mutant SERP-1 was constructed in which arginine 319 and asparagine 320 were changed to alanine residues. Mutation of the P1-P1' site in other serpins has been shown to abolish inhibitory activity (41). The resulting mutant protein, termed R319A/N320A, was tested for its ability to interact with the same panel of proteinases used to test wild type serpin, as described under "Experimental Procedures." The results are summarized in Table I. Fig. 2C shows that R319A/N320A did not react with plasmin, tPA, uPA, thrombin, or Xa (lanes 2-6, respectively). No high molecular weight complexes were observed; nor was there any evidence of the production of any smaller cleaved forms of the molecule. R319A/N320A was tested for inhibition of plasmin, tPA, and uPA in activity assays and showed no effect in either slow binding inhibition assays or by end point measurements of enzyme activity after prolonged incubation with an excess of the mutant serpin. The abolition of interactions with SERP-1 by the mutations in positions 319 and 320 confirms the importance of the P1-P1' residues for inhibition by SERP-1. Interestingly, R319A/N320A reacted with elastase and cathepsin G to yield a 50-kDa product (Fig. 2C, lanes 7 and 8), identical to the result seen when these enzymes were reacted with wild type SERP-1. This result suggests that these enzymes react at a site in or near the RCL but not at Arg319-Asn320. This was confirmed by Edman degradation sequencing of the 5-kDa fragment released upon reaction of wild type SERP-1 with cathepsin G and hNE. The results showed that both of these enzymes cleave after Ile314 in the RCL of wild type SERP-1, five residues upstream of Arg319. This result shows that cleavage of SERP-1 at a site close to P1-P1' in the RCL does not lead to formation of an inhibited complex (41).

Reaction Kinetics for Inhibition of Plasmin, tPA, uPA, Thrombin, and Xa by SERP-1-- Of the extensive panel of proteinases tested, only plasmin, tPA, uPA, thrombin, and Xa were observed to form SDS-stable complexes with SERP-1, although Lomas et al. have shown evidence for the formation of a complex between SERP-1 and C1s (17). We attempted to quantify the effectiveness of SERP-1 at inhibiting these six enzymes by investigating their reactions with SERP-1 using the techniques of slow binding inhibition kinetics (36, 42). Each proteinase was incubated with various concentrations of SERP-1 in the presence of a fixed concentration of an appropriate chromogenic proteinase substrate. The time-dependent inactivation of the enzyme through reaction with SERP-1 was monitored continuously by following the accompanying decrease in the rate of substrate turnover. Experiments were carried out at a low concentration of each enzyme (5-125 pM) so that the reaction could be followed for many hours without significantly depleting the chromogenic substrate. SERP-1 was always present at concentrations that were in >10-fold excess with respect to the proteinase, thus giving rise to pseudo-first-order conditions with respect to SERP-1. Fig. 3 shows a family of inhibition progress curves for the reaction of tPA with 0-20 nM SERP-1. The data show that higher concentrations of SERP-1 cause the reactions to approach the steady state more rapidly and give a lower velocity of substrate turnover at the steady state, indicating that a greater fraction of the enzyme has become inhibited through reaction with SERP-1. The rate of substrate turnover was observed to decrease to a finite steady-state level and not to zero as would be expected if inhibition by SERP-1 proceeded to completion, indicating the existence of one or more pathways by which the inhibited complex can break down to release active enzyme (36). Inhibition data were measured for each proteinase at at least six different SERP-1 concentrations chosen to span the range from slow or negligible inhibition to rapid and extensive reaction with SERP-1. Progress curves for reaction at each SERP-1 concentration were fitted to Equation 1 (36) to obtain values for the rate constant for the approach to the steady state, kobs, and the velocity of substrate turnover at the steady state, vs, as described under "Experimental Procedures." Fig. 3 (inset) shows the relationship between vs and the concentration of SERP-1 for reaction with tPA. The solid line represents the fit to Equation 2 and gives an apparent inhibition constant (IC50) of 360 pM for reaction with this enzyme.
v<SUB>s</SUB>=v<SUB>i</SUB>/<FENCE>1+[<UP>SERP-1</UP>]/<UP>IC</UP><SUB>50</SUB></FENCE> (Eq. 2)
The IC50 value determined for the reaction with each enzyme was corrected for the binding of chromogenic substrate in the assay as described by Stone and Hermans (36), to give a steady-state inhibition constant, KI(app), which represents the concentration of SERP-1 at which the enzyme is distributed equally between free and SERP-1-inhibited (complexed) forms. Values for KI(app) for all of the enzymes tested are given in Table II.


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Fig. 3.   Progress curves for inhibition of tPA by various concentrations of SERP-1. The enzyme (25 pM) was incubated with SERP-1 (0-20 nM) in the presence of the chromogenic substrate Chromozym-TPA (0.25 mM; 100 mM Tris-HCl, 100 mM NaCl, 2 mM CaCl2, 0.005% Triton X-100, pH 7.5, 37 °C). The reaction was followed at 405 nm. Curve a shows the rate of substrate turnover in the absence of SERP-1. Curves b-e show progress curves for the time-dependent inhibition of tPA activity by reaction with 2, 4, 10, and 20 nM SERP-1. Curve f shows the very slow background hydrolysis of Chromozym-TPA in the absence of tPA. Experimental progress curves are shown by the symbols, which for clarity show only every 10th time point; the solid lines represent the best fit of the data to Equation 1, from which were determined values for the initial and final velocities of substrate turnover, vi and vs, and the observed rate constant for the approach to steady state, kobs, at each concentration of SERP-1. The inset plot shows the dependence of the final steady state velocity of substrate turnover relative to uninhibited enzyme, vs/vi, determined from fitting individual progress curves from several experiments to Equation 1, on the concentration of SERP-1. The solid line represents the best fit to Equation 2, from which was determined a value of KI(app) = 0.14 nM for the inhibition of tPA by SERP-1 (see "Results").

                              
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Table II
Observed kinetic parameters for the reaction of SERP-1 with six inhibited proteinases
Values for the apparent inhibition constant, KI(app), the apparent second-order rate constant for inhibition, kapp, and the measured rate constant for dissociation of the inhibited complex, kd(exp), were determined in slow binding inhibition experiments as shown in Figs. 3 and 4 (100 mM Tris-HCl, 100 mM NaCl, 2 mM CaCl2, 0.005% Triton X-100, pH 7.5, 37 °C). Both KI(app) and kapp are corrected for the competitive binding of chromogenic substrate in the slow binding inhibition assays as described under "Results." Calculated values for the rate constant for dissociation of the inhibited complex, kd(calc), were determined using the equation kd(calc) = KI(app)kapp, as described under "Results." SI values were measured as shown in Fig. 5 (100 mM Tris-HCl, 100 mM NaCl, 2 mM CaCl2, 0.005% Triton X-100, pH 7.5, 37 °C). ND indicates that the given experimental data were not obtained.

Fig. 4 shows the dependence of kobs on the concentration of SERP-1 for reaction with uPA, tPA, plasmin, thrombin, and factor Xa. These plots are linear and show no evidence of hyperbolic curvature that would indicate the existence of one or more stable intermediate complexes forming prior to the rate-limiting step for formation of the final inhibited complex. The slopes of the best fits to the data in Fig. 4 give values for the apparent second-order rate constants for reaction of SERP-1 with plasmin, tPA, uPA, thrombin, and Xa of kapp = 4.8 ± 0.5 × 104, 4.3 ± 0.4 × 104, 5.0 ± 0.6 × 104, 2.6 ± 0.4 × 104, and 1.7 ± 0.5 × 103 M-1 s-1, respectively, after correction for competitive binding of the chromogenic substrate present during the reaction (36). Values for kapp and KI(app) for C1s were estimated from measurements made at a single SERP-1 concentration of 100 nM; the weak inhibition observed for this enzyme required amounts of SERP-1 that were too great to allow more detailed investigation of this reaction. The apparent second-order rate constants for these reactions are presented in Table II. The kinetic constants for reaction with plasmin, tPA, and uPA are in good agreement with those measured by Lomas et al., who reported kapp values of 3.4 × 104, 3.6 × 104, and 4.3 × 104 M-1 s-1, respectively, using crude viral supernatants (17). Our estimated value for C1s is also in reasonable agreement with their value of kapp = 1.3 × 103 M-1 s-1.


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Fig. 4.   Second-order replots for the reactions of SERP-1 with tPA (A), uPA (B), plasmin (C), thrombin (D), and factor Xa (E). For each enzyme, the observed rate constant for the approach to steady state, kobs, was determined at various concentrations of SERP-1 in slow binding inhibition experiments such as that shown in Fig. 3. The resulting values were corrected as described under "Discussion" to compensate for the competitive binding of chromogenic substrate in the slow binding inhibition assay. Plots of kobs (corrected) against the concentration of SERP-1 are linear, and the slopes give values for kapp, the apparent second-order rate constant for the reaction of each enzyme with SERP-1, which are given in Table II.

The rate at which each inhibited complex breaks down to release active enzyme was measured directly by incubating each enzyme with a small excess of SERP-1 at concentrations well above KI(app) and then diluting the resulting enzyme-serpin complex 50-fold, to a concentration below KI(app), into reaction buffer containing chromogenic substrate. The release of inhibition manifests itself as upward curvature in the progress curve, tending to a new steady-state velocity of substrate turnover commensurate with the lower concentration of SERP-1 present in the final reaction mixture. Equation 1 was used to fit the reactivation progress curves by nonlinear regression analysis, such that kobs = kd. Measured rate constants for the dissociation of complexes formed between SERP-1 and plasmin, tPA, thrombin, and Xa were kd = 3 ± 0.4 × 10-5, 6 ± 0.5 × 10-6, 2 ± 0.2 × 10-6, and 8 ± 0.4 × 10-6 s-1, respectively. Rate constants for the release of active enzyme from the inhibited complexes can also be calculated from the measured kapp and KI(app) values using the relationship kd KI(app)kapp. Values for kd that were calculated in this way are shown in Table II and agree well with the kd values that were measured directly.

Stoichiometries of Inhibition-- Fig. 2, A and B, show evidence for the formation of varying amounts of cleaved SERP-1 upon reaction with plasmin, tPA, uPA, thrombin, and Xa. The kd values shown in Table II suggest that the inhibited complexes formed with these enzymes dissociate too slowly for any significant amount of cleaved serpin to be released during the 2-h incubation period used for the measurements in Fig. 2A, although the possibility that the complex breaks down more readily during SDS-PAGE sample preparation cannot be discounted. An alternative explanation for the origin of the cleaved SERP-1 is the existence of a branched kinetic mechanism, illustrated in Scheme 1, such as has been demonstrated for other serpins (22, 26, 43-45). If this is the case for SERP-1, then the large variations in the proportion of cleaved serpin formed from reaction with the different enzymes in Fig. 2, A and B (from very little with uPA to >=  90% with thrombin) suggest the existence of correspondingly large variations in partitioning within the branched pathway for reaction with different enzymes. In order to determine whether the cleaved serpin seen in Fig. 2, A and B, arises from a branched mechanism and to quantify any differences in partitioning within such a mechanism, we measured the SI for the reactions of SERP-1 with uPA, plasmin, thrombin, and Xa. SI values were determined using a SERP-1 stock solution that had been accurately standardized by its absorption at 280 nm using an extinction coefficient determined by amino acid analysis and reacting it with enzyme solutions that had been accurately standardized by active site titration. Gel shift experiments, such as those described above, established that all of the SERP-1 protein in this standardized solution was active. Fig. 5 shows the titration of a fixed concentration of uPA with varying concentrations of SERP-1 and establishes that 1.4 ± 0.1 equivalents of SERP-1 are required to achieve exactly 100% inhibition, corresponding to an SI = 1.4 ± 0.1 (37). This value was consistent with the distribution of SERP-1 between cleaved and complexed forms that was estimated by Western blot or measured by quantitative densitometry of Coomassie Blue-stained SDS-polyacrylamide gels (data not shown). Fig. 5 also shows that titrations of plasmin, thrombin, and factor Xa with SERP-1 similarly give rise to linear titration curves, resulting in the SI values shown in Table II. The values range from the relatively low value found for reaction with uPA to a high of SI = 13 ± 3 for reaction with thrombin. The active site titrant MUGB did not react with tPA in our hands, and, lacking an accurate method for the standardization of this enzyme, we were unable to perform a titration against SERP-1 of the type shown in Fig. 5. However, the roughly equal intensities of the bands for cleaved and complexed SERP-1 seen in Western blots suggest that for reaction with tPA, SI approx  2 (limits were 1.5-3).


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Fig. 5.   Stoichiometry of inhibition for the reactions of uPA (open circle ), plasmin (), factor Xa (down-triangle) and thrombin (triangle ) with SERP-1. Fixed concentrations of each enzyme were incubated with SERP-1 at various molar ratios for 1 h at 37 °C (100 mM Tris-HCl, 100 mM NaCl, 2 mM CaCl2, 0.005% Triton X-100, pH 7.5), except for Xa, for which a 10-h incubation was required to reach the final level of inhibition. At the end of the incubation, residual proteinase activity in each reaction was determined in a chromogenic substrate assay. In all cases, control experiments at longer reaction times were performed to show that reaction was complete and that incubation under these conditions did not cause any significant loss of activity in the enzyme only control. The SI for each proteinase corresponds to the minimum number of equivalents of SERP-1 required to achieve complete inhibition of enzyme activity and was determined by extrapolating residual proteinase activity to its intercept on the x axis as shown by the solid lines in the figure. SI values for each enzyme were measured a minimum of three times in at least two independent experiments and are given in Table II.

    DISCUSSION
Top
Abstract
Introduction
Procedures
Results
Discussion
References

The gel shift experiments shown in Fig. 2 confirm the observations of Lomas et al. (17), made using wild type SERP-1 from unfractionated viral supernatant, that this secreted viral serpin inhibits tPA, uPA, and plasmin. We additionally identify factor Xa and thrombin as in vitro targets for SERP-1 inhibition. Both thrombin and Xa were reported by Lomas et al. to cleave SERP-1 without forming any detectable inhibited complex. Our subsequent kinetic analysis showed thrombin to be among the most reactive targets of SERP-1 inhibition; however, the high SI seen for this enzyme made the observation of an inhibited complex on Western blots quite difficult. Another difference between our results and those of Lomas et al. was that in the previous study no reaction was seen between SERP-1 and cathepsin G (17), whereas in the present study reaction at a somewhat higher enzyme:SERP-1 ratio showed that cathepsin G does react with SERP-1, cleaving at a single site in the RCL. A final difference between our results and those of Lomas et al. is that in the present study we were unable to detect any stable inhibited complex between SERP-1 and C1s on Western blots, although we did find evidence for weak inhibition of this enzyme by SERP-1 in kinetic experiments. The discrepancies between the two studies illustrate that the ability to observe a stable inhibited enzyme-serpin complex on SDS gels can be quite sensitive to the relative and absolute concentrations of serpin and enzyme used, to the duration and conditions of the reaction, and possibly also to the purity of the serpin preparation.

Thrombin was shown, by sequencing of the cleaved serpin, to react with SERP-1 at the predicted site between Arg319 and Asn320. All of the other enzymes that were found to be inhibited by SERP-1 also have a primary specificity for cleaving after a basic residue, and the fact that mutating Arg319-Asn320 to Ala-Ala abolished the reactivity of SERP-1 toward all of these enzymes argues strongly that they all act at this position in the RCL. The failure of these enzymes to be inhibited by the mutant SERP-1 is unlikely to be due to improper folding or long range disruption of the serpin structure because of the location of the mutations in a flexible exposed loop (41). This assumption is supported by the observation that cathepsin G and elastase, which were shown to act at Ile314-Thr315, just five residues upstream of Arg319 in the RCL, appear on Western blots to display the same highly specific single cleavage of wild type and the mutant SERP-1.

Table II contains rate constants for the formation of the inhibited complex and for its decomposition, together with SI values, for reaction with the six proteinases that were found to be inhibited by SERP-1. These results comprise an unusually comprehensive and quantitative data set for direct comparison of the reactivity of a single serpin with a series of target proteinases. In order to properly evaluate the factors that govern the specificity of SERP-1 for the enzymes tested, we must consider the data in Table II in terms of the branched kinetic mechanism shown in Scheme 1. The minimal mechanism in Scheme 1 incorporates the basic features of the serpin inhibition mechanism as established for the reactions of other serpin-proteinase pairs (22, 23). The applicability of this branched mechanism to the reaction of SERP-1 with the enzymes listed in Table II is demonstrated by the observation that, in every case, reaction leads to the formation of cleaved SERP-1 concomitantly with the formation of the inhibited enzyme-serpin complex, leading to SI values that are in all cases greater than 1 (37, 46). The precise chemical and structural nature of the partitioning intermediate, [EI], in the serpin inhibition mechanism remains in question. Published suggestions include the possibility that [EI] is an acyl-enzyme intermediate (39, 47), a related covalent species trapped at the tetrahedral intermediate stage (48), or possibly a noncovalent intermediate that precedes P1-P1' bond cleavage (29). Our data provide no evidence to indicate which of these possibilities applies to the reactions of SERP-1. Despite the undefined nature of the partitioning intermediate, we can nevertheless use SI to calculate the partitioning ratio between the pathway that leads to inhibited complex (EI*) and the pathway that leads directly to the release of cleaved SERP-1 (I'), using the relation SI = 1 + k3/k4 (22). Partitioning ratios for the reactions of SERP-1 with plasmin, tPA, uPA, thrombin, and Xa are shown in Table III. In order for us to interpret the remaining data in Table II in terms of the branched kinetic mechanism shown in Scheme 1, we must correct the apparent second-order rate constants, kapp, to account for the effect of partitioning, as described by Hood et al. (49). Multiplying kapp by SI compensates for the fact that for an SI of 10, for example, only 1 out of every 10 reactions between enzyme and serpin leads to the formation of the inhibited complex, EI*. Because of this effect, the true second-order rate constant for reaction between E and I in such a case is 10 times higher than the apparent rate constant for the formation of EI* that is given by kapp (49). The true second-order rate constants for reaction of SERP-1 with each of the six proteinases for which we have kinetic data, calculated from kinh = kapp × SI, are collected in Table III. The apparent steady-state KI(app) values from Table II must also be corrected for the effect of partitioning, in this case by dividing by a factor of SI, if they are to reflect the balance between the true rate constants for the formation and decomposition of EI*(49). The corrected values for KI are also given in Table III.

                              
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Table III
Final kinetic parameters for the reaction of SERP-1 with six inhibited proteinases after correction for the effects of partitioning in the branched kinetic mechanism
Values for the true second-order rate constant for inhibition, kinh, were calculated from the kapp values in Table II by correcting for the effect of partitioning in the branched kinetic mechanism using the formula kinh = kappSI, as described under "Discussion." Similarly, true KI values were calculated from the KI(app) values in Table II using the formula KI = KI(app)/SI. Values for kd are not affected by partitioning and so were taken directly from Table II; the directly determined experimental values are used except for reaction with uPA and with C1s, for which the calculated values (kd(calc)) are given. The partitioning ratio k3/k4 was calculated using the formula SI = 1 + k3/k4, as described in the text. ND indicates that the experimental data were not obtained (see "Discussion").

The data in Table III show that the rates at which SERP-1 reacts with the six proteinases listed vary from 3.4 × 105 M-1 s-1 for reaction with thrombin to 5.4 × 103 M-1 s-1 for reaction with Xa, with C1s having an estimated value for kinh that is an order of magnitude lower still. These variations in kinh, which span 3 orders of magnitude, account for most of the variations in KI. Values for KI vary from 10 pM for thrombin to 1.3 nM for Xa, and span ~4 orders of magnitude if the estimated value of KI of ~100 nM for C1s is included. Interestingly, values for kd. vary over a relatively narrow range, indicating that the kinetic barrier for decomposition of EI* is less sensitive to differences in the structure and catalytic properties of the proteinase than are the other kinetic parameters for the reaction. Of the reactions included in Table III, only that with thrombin approaches the second-order rate constants seen for reactions between serpins and the proteinases believed to represent their specific targets in vivo, which typically exceed 105 M-1 s-1 (50, 51). Reaction with thrombin, however, is characterized by a high partitioning ratio, indicating that the majority of reactions between SERP-1 and this enzyme are nonproductive and lead to cleavage and inactivation of SERP-1 rather than to inhibition of the enzyme. In general, the partitioning ratios in Table III are higher than is typically seen for the reaction of serpins with their specific target proteinases (37). Thus, none of the enzymes in Table III appear to possess all of the characteristics of fast inhibition, low SI, and slow dissociation of the inhibited complex that would be anticipated to exist for the reaction of SERP-1 with a true in vivo target (50, 52).

The data in Tables II and III allow us to draw several conclusions about which steps in the inhibition mechanism are involved in governing the inhibitory specificity of SERP-1. Under conditions in which the serpin is present at concentrations significantly higher than both KI and the concentration of its proteinase target, the specificity of SERP-1 is primarily governed by the rate of its initial reaction with enzyme; even for an enzyme that reacts with a high SI, the amount of serpin lost through nonproductive cleavage will be insignificant. However, even under conditions of excess serpin, variations in SI may influence inhibitory specificity. An example of this is provided by thrombin, for which, as a consequence of its high SI, inhibition is effectively slower than inhibition of uPA or tPA despite the fact that thrombin undergoes a substantially faster initial reaction with SERP-1. Variations in SI assume a larger role in governing inhibitory specificity under conditions in which the serpin is present at comparable concentration to its target or targets. Under these conditions, inhibition of proteinases for which a high SI is observed will be partial at best, irrespective of how fast the reaction occurs, because most of the serpin will be inactivated through cleavage at the RCL. Moreover, it is possible that the presence in vivo of a highly reactive proteinase with a high SI would reduce the likelihood that any other proteinase present will become inhibited, by competing for serpin and irreversibly cleaving it to the inactive form, I'. Finally, at concentrations of serpin that are very low, the dissociation rate of EI* may also begin to influence inhibitory specificity. For example, plasmin and tPA are both inhibited by SERP-1 at similar rates and have similar values for SI. However, at concentrations of ~100 pM, SERP-1 would be expected to inhibit tPA much more effectively than plasmin, if present in excess over both, because at this concentration it exceeds the KI of 70 pM for reaction with tPA but is well under the KI of 220 pM for reaction with plasmin. The difference in KI values for these two enzymes arises from the 5-fold faster rate at which EI* decomposes in the case of plasmin. The results in Table III therefore suggest that at high concentrations of SERP-1, inhibitory specificity is predominantly governed by kapp, which depends on kinh and k3/k4. At concentrations of serpin that are comparable with that of the target proteinase, specificity becomes much more strongly dependent on the magnitude of k3/k4. Finally, at very low serpin concentrations that begin to approach KI, specificity can also be influenced by the magnitude of kd. Although many serpins, most notably those that circulate in the blood, are present at very high concentrations in vivo, it is likely that SERP-1 achieves its anti-inflammatory effect at much lower concentrations, acting at levels comparable with those of its target proteinases (8, 14, 15). This may also be true in other biologically important situations, such as the inhibition of intracellular caspases by CrmA (52) or other serpin inhibitors of apoptosis.

Initial interest in SERP-1 was sparked by the discovery that it is a virulence factor of myxoma virus, acting in concert with other secreted and intracellular receptor mimics and inhibitors to cause severe immune dysfunction in the host (12). Furthermore, SERP-1 was observed to act as an anti-inflammatory agent during myxoma virus infection (8) and when administered as a purified protein in two in vivo models of inflammation (14, 15). Therefore, the target proteinases of SERP-1 are likely to be critical to initiating or propagating the early inflammatory signal in response to virus infection. The low doses of SERP-1 required for efficacy in various in vivo models (14, 15) suggest that its target proteinase or proteinases are present at exceedingly low levels at the site of action. Although the results obtained in this study do not serve to identify the proteinase target or targets for SERP-1 that are responsible for its in vivo effects, they confirm that it is likely to be a trypsin-like proteinase with specificity for cleaving after a basic residue. In addition, our results shed some light on the mechanistic parameters that govern the inhibitory specificity of SERP-1, while highlighting the fact that the specificity of a serpin for inhibiting one or another proteinase may be significantly influenced by reaction conditions at the site of action and particularly by the relative concentrations of proteinase and serpin. The ability of SERP-1 to dampen the inflammatory response to the virus infection offers clues to the roles that serine proteinases may be playing in these processes. A better understanding of the underlying mechanisms may help in the development of anti-inflammatory drugs based on serpins and small molecule proteinase inhibitors.

    ACKNOWLEDGEMENTS

We thank Susan Foley for sequencing the C-terminal cleaved peptides of SERP-1; Joe Budman for testing the activity of SERP-1 against the alternative pathway C3 convertase; Michele McKay for determining the extinction coefficient of SERP-1; Betty Chao for performing an HPLC analysis of purified vaccinia virus SERP-1; Carley Hodgkinson for exceptional technical assistance; and Peter Gettins, Christopher Borysenko, and the late Stuart Stone for thought-provoking discussions. We also thank Rem Baciu, Kam Cheung, Konrad Miatkowski, Claire Moore, Renee Shapiro, Fred Taylor, and, in particular, Steve Fawell and Leona Ling, all of Biogen, Inc., for helpful discussions and support.

    FOOTNOTES

* This work was supported by the Medical Research Council of Canada, the National Cancer Institute (Canada), and Biogen, Inc.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Recipient of an Alberta Heritage Foundation for Medical Research Studentship and a University of Alberta Dissertation Fellowship.

** A Medical Research Council of Canada Senior Scientist. To whom correspondence should be addressed: Robarts Research Institute, 1400 Western Rd., SDRI 133, London, Ontario N6G 2V4, Canada. Tel.: 519-663-3184; Fax: 519-663-3847. 

The abbreviations used are: RCL, reactive center loop; tPA, tissue-type plasminogen activator; uPA, urokinase-type plasminogen activator; hNE, human neutrophil elastase; SI, stoichiometry of inhibitionMUGB, 4-methylumbelliferyl p-guanidinobenzoate4-MU, 4-methylumbelliferoneTBS, Tris-buffered salinePAGE, polyacrylamide gel electrophoresisBGMK, baby green monkey kidney epithelial cell lineCAPS, 3-cyclohexylamino-1-propanesulfonic acidHPLC, high pressure liquid chromatographyFPLC, fast protein liquid chromatography.

2 M. McKay, unpublished data.

    REFERENCES
Top
Abstract
Introduction
Procedures
Results
Discussion
References

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