Mitotic Arrest with Nocodazole Induces Selective Changes in the Level of O-Linked N-Acetylglucosamine and Accumulation of Incompletely Processed N-Glycans on Proteins from HT29 Cells*

(Received for publication, December 10, 1996, and in revised form, January 23, 1997)

Robert S. Haltiwanger Dagger and Glenn A. Philipsberg

From the Department of Biochemistry and Cell Biology, Institute for Cell and Developmental Biology, State University of New York, Stony Brook, New York 11794-5215

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES


ABSTRACT

O-Linked N-acetylglucosamine (O-GlcNAc) is a ubiquitous and abundant protein modification found on nuclear and cytoplasmic proteins. Several lines of evidence suggest that it is a highly dynamic modification and that the levels of this sugar on proteins may be regulated. Previous workers (Chou, C. F., and Omary, M. B. (1993) J. Biol. Chem. 268, 4465-4472) have shown that mitotic arrest with microtubule-destabilizing agents such as nocodazole causes an increase in the O-GlcNAc levels on keratins in the human colon cancer cell line HT29. We have sought to determine whether this increase in glycosylation is a general (i.e. occurring on many proteins) or a limited (i.e. occurring only on the keratins) process. A general increase would suggest that the microtubule-destabilizing agents were somehow affecting the enzymes responsible for addition and/or removal of O-GlcNAc. Our results suggest that the changes in O-GlcNAc induced by nocodazole are selective for the keratins. The levels of O-GlcNAc on other proteins, including the nuclear pore protein p62 and the transcription factor Sp1, are not significantly affected by this treatment. In agreement with these findings, nocodazole treatment caused no change in the activity of the enzymes responsible for addition or removal of O-GlcNAc as determined by direct in vitro assay. Interestingly, nocodazole treatment did cause a dramatic increase in modification of N-glycans with terminal GlcNAc residues on numerous proteins. Potential mechanisms for this and the change in glycosylation of the keratins are discussed.


INTRODUCTION

O-Linked N-acetylglucosamine (O-GlcNAc)1 is a ubiquitous and abundant form of post-translational modification found on nuclear and cytoplasmic proteins (1-3). It consists of the monosaccharide N-acetylglucosamine attached to the hydroxyl groups of serines and/or threonines. Although over 50 proteins bearing this modification have been identified to date, no example of a specific function for this modification on any of these proteins has yet been demonstrated. Nonetheless, these proteins share some common features that may suggest a role. Significantly, most of these proteins are also known to be phosphoproteins (1-3). Since both phosphate and GlcNAc modify the hydroxyl groups of serines and threonines, it has been proposed that the sugar may function by blocking sites of phosphorylation (4). Several recent reports demonstrating competition between glycosylation and phosphorylation on individual proteins have added support to this concept (5, 6). Modifying a protein with a neutral sugar such as O-GlcNAc could have significantly different effects on the protein compared with phosphorylation. Thus, O-GlcNAc modification of proteins may add an additional level of control to protein phosphorylation by constitutively blocking phosphorylation sites.

In addition to competing with phosphate, several lines of evidence suggest that O-GlcNAc may be a regulated modification much like phosphorylation. For example, in several cases, O-GlcNAc has been shown to turn over more rapidly than the proteins it modifies (7, 8). This suggests that the sugar is dynamically added to and removed from proteins during their lifetimes. Enzymes capable of addition and removal of the sugar from proteins have been purified and characterized (9, 10). They exist as large multisubunit soluble proteins in the cytoplasm of cells. Thus, a system capable of regulated addition and removal of the sugar exists. In addition to simply turning over more rapidly than the protein, changes in the levels of glycosylation induced by various stimuli have been observed in two separate systems. Kearse and Hart (11) showed that mitogenic activation of either isolated T lymphocytes or T-cell hybridomas resulted in rapid (<1 h) changes in the level of glycosylation of several nuclear and cytoplasmic proteins. Subsequently, Chou and Omary (12, 13) demonstrated that mitotic arrest of a human colon cancer cell line (HT29) with okadaic acid or microtubule-destabilizing agents (such as nocodazole) resulted in an increase in glycosylation of keratins 8 and 18. In addition, Chou and Omary (13) observed increased glycosylation of proteins in a crude nuclear extract as well as on several known plasma membrane proteins. The increase in terminal GlcNAc residues of the plasma membrane proteins could be mimicked by brefeldin A, a Golgi disrupter, suggesting that these changes were on extracellular glycans, not O-GlcNAc. The brefeldin A treatment had no effect on the glycosylation of keratins 8 and 18. Nonetheless, the increase in terminal GlcNAc residues on proteins from the nuclear extracts of these cells suggested that nocodazole may cause a general increase in the levels of O-GlcNAc on proteins in these cells.

We have sought to determine whether the increase in glycosylation of the keratins in HT29 cells induced by nocodazole is a selective or a general event. If there is an induction of an O-GlcNAc-transferase or an inhibition of an O-GlcNAc'ase by a stimulus such as nocodazole, one would expect to see changes in the level of glycosylation of several proteins. This appears to be what was observed by Kearse and Hart (11) in mitogenically activated T lymphocytes. We have examined the glycosylation of whole cell extracts as well as several specific proteins (keratins, a nuclear pore protein, and the transcription factor Sp1) in nocodazole-treated HT29 cells. Our data suggest that the change in glycosylation of the keratins is a selective event. We saw no major change in O-GlcNAc levels on the other proteins we examined. In agreement with Chou and Omary (12, 13), we saw a generalized increase in terminal GlcNAc residues on numerous proteins upon nocodazole treatment. We have demonstrated that this increase is largely due to an accumulation of incompletely processed N-glycans in nocodazole-treated cells.


EXPERIMENTAL PROCEDURES

Materials

Nocodazole, bovine milk galactosyltransferase, protein G-agarose, and ovalbumin were from Sigma. Galactosyltransferase was autogalactosylated prior to use as described (14). Peptide N-glycosidase F (PNGase F) was purified from culture filtrate of Flavobacterium menigisepticum as described (15). Anti-keratin monoclonal antibody (L2A1) ascites fluid was generously provided by Dr. Bishr Omary (Stanford University) (16). Anti-nuclear pore monoclonal antibody (414) ascites fluid was obtained from Babco (Berkeley, CA), and an isotype-matched control IgG (ascites fluid) was obtained from Sigma (mineral oil plasmacytoma 21). Rabbit anti-human Sp1 polyclonal antibody (PEP2) and competing peptide were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Horseradish peroxidase-conjugated goat anti-mouse IgG was obtained from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA). The human colon cancer cell line HT29 was obtained from the American Type Culture Collection (Rockville, MD). Dulbecco's modified Eagle's medium, fetal bovine serum, penicillin/streptomycin, and trypsin/EDTA were obtained from Life Technologies, Inc. UDP-[6-3H]galactose (50-60 Ci/mmol), UDP-[6-3H]GlcNAc (25.8 Ci/mmol), and EN3HANCE were from DuPont NEN. The disaccharide standards (galactose-beta 1,3-N-acetylgalactosamine, galactose-beta 1,3-N-acetylglucosamine, and galactose-beta 1,4-N-acetylglucosamine) were obtained from Sigma. The alditol derivatives of these standards were prepared by reduction with sodium borohydride as described (17). The peptide YSDSPSTST was synthesized at the Center for the Analysis and Synthesis of Macromolecules, State University of New York at Stony Brook. All electrophoresis reagents were from Bio-Rad. Enhanced chemiluminescence reagents were from Amersham Corp. All other reagents were of the highest quality available.

Cell Culture and Nocodazole Treatments

HT29 cells were maintained in a humidified incubator with 5% CO2 in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum and penicillin/streptomycin (complete Dulbecco's modified Eagle's medium). Nocodazole treatments were performed by replacing the media on subconfluent plates (50-75% confluent) with fresh complete media with or without 0.5 µg/ml nocodazole. At appropriate time points, the cells were washed once with 10 mM Tris, pH 7.5, 0.15 M NaCl (Tris-buffered saline) and removed from the plates with trypsin/EDTA. The cells were then washed three times with cold Tris-buffered saline, counted, and frozen at -80 °C until use.

Galactosyltransferase Labeling of Whole Cell Extracts

All galactosyltransferase labelings were performed as modifications of described procedures (14). Cell pellets prepared as described above were extracted with hot 1% SDS (0.5 ml/107 cells) for 5 min at 100 °C. Chromatin was sheared by repeated passage of the extracts through a 25-gauge needle. Insoluble material was removed by centrifugation at 10,000 × g for 10 min at 4 °C. Equivalent protein aliquots (100 µg) were precipitated with 8 volumes of acetone for >4 h at -20 °C. Protein pellets were collected by centrifugation and solubilized in 50 µl of 1% SDS by heating to 100 °C for 5 min. After cooling, the samples were diluted to 0.5 ml with galactosyltransferase labeling buffer (final concentrations: 50 mM Hepes, pH 7.4, 5 mM MnCl2, 100 mM galactose) containing 2% (w/v) Triton X-100. At this concentration of detergents (0.1% SDS and 2% Triton X-100), galactosyltransferase is fully active (data not shown). The labeling was conducted under saturating conditions for galactosyltransferase (41.3 milliunits) and UDP-[6-3H]galactose (2 µCi) at 37 °C for 1 h. Unincorporated radiolabel was separated from the labeled proteins by gel filtration chromatography (Sephadex G-50) as described (14). The protein fraction was concentrated by lyophilization and acetone precipitation prior to analysis by SDS-PAGE and fluorography.

Galactosyltransferase Labeling of Keratins

For galactosyltransferase labeling of keratins, cells were extracted essentially as described by Chou et al. (7). Briefly, cell pellets prepared as described above were extracted for 30 min on ice with 10 mM sodium phosphate, pH 7.4, 0.15 M NaCl (phosphate-buffered saline) containing 0.5% (w/v) Nonidet P-40 and protease inhibitor cocktails 1 and 2 (Pic 1 and Pic 2) (0.5 ml/107 cells) (14). Insoluble material was removed by centrifugation at 10,000 × g for 10 min at 4 °C. Equivalent amounts of protein (100 µg) of the extracts were labeled with galactosyltransferase and UDP-[6-3H]galactose as described above. The labeling was stopped by the addition of 25 mM EDTA, and the keratins were immunoprecipitated from the labeling mixture using monoclonal antibody L2A1 and protein G-agarose as described (18).

Galactosyltransferase Labeling of Nuclear Pore Proteins and Sp1

For galactosyltransferase labeling of nuclear pore proteins and Sp1, cells were extracted with RIPA buffer (50 mM Tris, pH 8.0, 0.15 M NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1% Nonidet P-40) containing Pic 1 and Pic 2 for 30 min on ice. Aggregates were broken by passage of the lysates through a 25-gauge needle several times. Insoluble material was removed by centrifugation at 10,000 × g for 10 min at 4 °C. All immunoprecipitations were performed on extract containing 1 mg of protein. Nuclear pore proteins were immunoprecipitated with monoclonal antibody 414, and Sp1 was immunoprecipitated with polyclonal antibody PEP2 essentially as described (18). A control for the monoclonal antibody 414 immunoprecipitations was performed with isotype-matched mouse IgG ascites fluid. The Sp1 immunoprecipitations were done with and without the control peptide as described by the manufacturer (Santa Cruz Biotechnology). Immune complexes were collected with protein G-agarose and washed five times with RIPA buffer. Galactosyltransferase labeling was performed on the immune complexes bound to the protein G-agarose beads in 0.5 ml with galactosyltransferase labeling buffer containing 2% Triton X-100, 41.3 milliunits of galactosyltransferase, and 2 µCi of UDP-[6-3H]galactose for 1 h at 37 °C. The labeled immune complexes were then washed twice with RIPA buffer and analyzed by SDS-PAGE and fluorography.

Carbohydrate Analysis

PNGase F digestions of galactosyltransferase-labeled protein fractions were performed as described (19). The released glycans were separated from the protein fraction by gel filtration on Sephadex G-50 (1 × 50 cm) in 50 mM ammonium formate, 0.1% SDS, 0.02% sodium azide. Quantitation was performed by measuring the radioactivity of aliquots from fractions collected. The protein fractions were lyophilized and acetone-precipitated. Alkali-induced beta -elimination was performed on the precipitated, PNGase F-digested protein fractions as described (19). Released saccharides were separated from the protein fraction by gel filtration on Sephadex G-50 (1 × 50 cm) as described above. The released saccharide fractions were desalted by passage over Dowex 50-X8 (H+ form) and Dowex 1-X8 (formate form). The desalted saccharides were subjected to size fractionation on a Superdex peptide fast protein liquid chromatography column (Pharmacia Biotech Inc.). The column was chromatographed at 0.5 ml/min in Milli Q water (Millipore Corp.) and was calibrated with partially hydrolyzed dextran standards detected using an in-line Rainin refractive index monitor (20). The pooled saccharide fraction from the Superdex column was then separated by high pressure anion-exchange chromatography analysis on a CarboPac MA-1 column (4 × 25 mm; Dionex Corp.) with disaccharide standards as described (8).

Assay of O-GlcNAc-transferase, O-GlcNAc'ase, and UDP-HexNAc Levels

Cytosolic fractions for enzyme assays were made by sonicating cell pellets in 20 mM Tris, pH 7.5, 0.25 M sucrose containing Pic 1 and Pic 2 (2 × 107 cells/ml). Sonication was for 10 s on ice with a Branson Model 185E probe sonicator set on level 3. Sonicates were centrifuged at 10,000 × g for 10 min at 4 °C. The supernatants were then centrifuged at 100,000 × g for 60 min at 4 °C. The supernatants (cytosol) were desalted on 1-ml Sephadex G-50 columns as described (9) prior to assay. O-GlcNAc-transferase assays were performed essentially as described (9) using the SP-Sephadex assays and YSDSPSTST as the acceptor peptide, although the final volume of the assay was reduced to 25 µl. The O-GlcNAc'ase activity was assayed as described (10) using p-nitrophenyl-beta -N-acetylglucosamine as substrate. O-GlcNAc'ase assays were performed at pH 6.5 and in the presence of 50 mM GalNAc to inhibit lysosomal hexosaminidases. Similar O-GlcNAc'ase assays were also performed with [3H]GlcNAc-labeled YSDSPSTST as described (10). Essentially identical results were obtained.

UDP-HexNAc levels were determined by high pressure liquid chromatography analysis of perchloric acid extracts of cell pellets as described (21). Since UDP-GlcNAc and UDP-GalNAc do not separate using this technique, data are presented as total UDP-HexNAc levels. We have analyzed the ratio of UDP-GlcNAc to UDP-GalNAc by performing carbohydrate analysis after acid hydrolysis (22) of the UDP-HexNAc fraction. The ratio of GlcNAc to GalNAc in the pool is ~2:1, consistent with previously published results (21).

Other Methods

All gels were 10% SDS-polyacrylamide gels as described by Laemmli (23). Western blots were performed as described (18). Detection on Western blots was by ECL as described by the manufacturer (Amersham Corp.). Protein was estimated using the BCA assay (Pierce).


RESULTS

Nocodazole Induces a Dramatic Increase in the Level of O-GlcNAc on Keratin 18

Chou and Omary (12) demonstrated that nocodazole treatment of the human colon cancer cell line HT29 resulted in a significant increase in the glycosylation of keratins 8 and 18. We sought to extend this observation to determine whether the increase in glycosylation was a general phenomenon, or if the change was specific for the keratins. To demonstrate that nocodazole treatment of the HT29 cells does indeed cause a change in the level of O-GlcNAc on the keratins, a time course of nocodazole treatment was performed (Fig. 1). Cells were lysed at the indicated times, and portions of each lysate were labeled with galactosyltransferase under conditions where all accessible terminal GlcNAc residues are modified with [3H]galactose (see "Experimental Procedures"). Keratins 8 and 18 were then isolated by immunoprecipitation and examined by SDS-PAGE and fluorography. Glycosylation of keratin 18 was barely detectable in the untreated cells, but it increased markedly between 8 and 24 h of nocodazole treatment (Fig. 1A), coincident with mitotic arrest (12). Under these same conditions, we saw very little glycosylation of keratin 8. Chou and Omary (12) also reported significantly less glycosylation of keratin 8 than keratin 18. During the time course, there was essentially no change in the level of the keratins themselves (Fig. 1B), indicating that there is a true increase in glycosylation of keratin 18 and not just an increase in the level of the protein. We also saw an increase in the labeling of a protein that coprecipitates with the keratins. This protein is most likely an N-glycosylated protein called KAP85 (13). Thus, as described by Chou and Omary (12), nocodazole induces a dramatic increase in the level of glycosylation of keratin 18. 


Fig. 1. Nocodazole induces an increase in glycosylation of keratin 18 in HT29 cells. HT29 cells were treated with nocodazole for the indicated times and lysed. Proteins in the extract (100 µg) were labeled with galactosyltransferase as described under "Experimental Procedures." Keratins were immunoprecipitated out of the galactosylated samples and separated by SDS-PAGE. The migration positions of KAP85 (13), keratins 8 and 18 (K8 and K18), and the IgG heavy chain are indicated. Molecular masses (in kilodaltons) of standard proteins are indicated between the panels. A, fluorograph of the gel; B, Coomassie Blue stain of the gel.
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Nocodazole Induces a Significant Increase in Terminal GlcNAc Residues on Numerous Proteins in HT29 Cells

To determine whether the effect of nocodazole on the keratins was a general or a selective event, we examined the glycosylation of proteins from crude lysates of HT29 cells. Lysates of HT29 cells were galactosyltransferase-labeled exactly as described above except that no immunoprecipitation was performed. A dramatic increase in the labeling of numerous protein species occurred between the 8- and 24-h time points (Fig. 2A), coincident with the increase in the glycosylation of keratin 18 shown in Fig. 1. During this same period, no significant changes were seen in the levels of the proteins themselves (Fig. 2B). These results demonstrate that mitotic arrest with nocodazole induces a large increase in terminal GlcNAc residues on numerous proteins from HT29 cells. Interestingly, the labeling of at least one protein decreased upon nocodazole treatment (Fig. 2A, asterisk). We believe that this decrease is due to a decrease in the solubility of this protein under the extraction conditions used (phosphate-buffered saline and 0.5% Nonidet P-40). The decrease is no longer observed when cell extracts are made with either RIPA buffer or 1% SDS (see Fig. 4). For this reason, subsequent lysates were all made with either RIPA buffer or 1% SDS.


Fig. 2. Nocodazole induces an increase in terminal GlcNAc residues on numerous proteins in whole cell lysates of HT29 cells. Proteins from nocodazole-treated HT29 cells were extracted and labeled as described in the legend to Fig. 1. The samples were then resolved by SDS-PAGE. The asterisk marks the migration position of a protein for which the level of glycosylation decreases upon nocodazole treatment. Molecular masses (in kilodaltons) of standard proteins are indicated between the panels. A, fluorograph of the gel; B, Coomassie Blue stain of the gel.
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Fig. 4. Nocodazole does not induce major changes in the profile of O-GlcNAc-modified proteins. Galactosyltransferase-labeled samples (25 µg of protein) from control and nocodazole-treated HT29 cells were treated with and without PNGase F (as described in the legend to Fig. 3) and separated by SDS-PAGE. Molecular masses (in kilodaltons) of standard proteins are indicated between the panels. A, fluorograph of the gel; B, Coomassie Blue stain of the gel.
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Increase in Terminal GlcNAc Residues Is Due to N-Glycans and Not O-GlcNAc

Chou and Omary (13) had demonstrated that nocodazole could induce an increase in the level of terminal GlcNAc residues on cytokeratins, proteins in nuclear extracts, and extracellular proteins. Since the changes in GlcNAc on extracellular proteins were likely to result from changes in N-glycans, we sought to determine whether the increase in terminal GlcNAc residues shown in Fig. 2 was due to changes in O-linked and/or N-linked GlcNAc. We used the enzyme PNGase F to differentiate between GlcNAc linked to proteins through N-glycans or other means. In untreated cells, essentially all of the radiolabel was resistant to PNGase F digestion (Fig. 3A, compare 0 h - PNGase F with 0 h + PNGase F), implying that the majority of the GlcNAc residues are O-linked. In contrast, the nocodazole-treated samples showed significant sensitivity to the PNGase F digestion (Fig. 3A, compare 32 h - PNGase F with 32 h + PNGase F). In fact, the increase in galactose incorporation caused by the nocodazole treatment could be completely accounted for by the increase in PNGase F-sensitive (i.e. N-linked) material. Thus, nocodazole appeared to induce an increase in terminal GlcNAc residues on N-glycans in these cells, but it caused no significant changes in the PNGase F-resistant (i.e. O-linked) material.


Fig. 3. Increase in terminal GlcNAc residues induced by nocodazole is largely due to GlcNAc residues on N-glycans. A, HT29 cells were treated with nocodazole for 0 or 32 h, lysed with 1% SDS, and labeled with galactosyltransferase as described under "Experimental Procedures." Samples were treated or mock-treated with PNGase F, and the radioactivity remaining associated with the protein fraction was quantitated. B, PNGase F-resistant material (from A) was subjected to beta -elimination to release the O-linked sugars from the proteins. Released and resistant fractions were separated by gel filtration and quantitated. C, shown is the Superdex gel filtration profile of a representative sample of material released by the beta -elimination (from B; identical results with either the 0- or 32-h nocodazole sample). The migration positions of hydrolyzed dextran standards are indicated (diamonds). D, shown is the high pressure anion-exchange chromatography profile (MA-1 column) of a representative sample migrating as a disaccharide on the Superdex column (from C). The migration positions of the following standards are indicated: D-galactose-beta 1,3-D-N-acetylgalactosaminitol (1), D-galactose-beta 1,3-D-N-acetylglucosaminitol (2), and D-galactose-beta 1,4-D-N-acetylglucosaminitol (3).
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To confirm that the labeled sugars remaining on the proteins after PNGase F digestion were in the form of O-GlcNAc, the PNGase F-resistant material from both the control and nocodazole-treated samples was subjected to alkali-induced beta -elimination. This caused the majority (>90%) of the radioactivity to be released from the protein fraction (Fig. 3B), indicating that the PNGase F-resistant radioactivity was O-linked to the protein. To determine the structure of the material released by the beta -elimination, the samples were subjected to size (Fig. 3C) and high pressure anion-exchange chromatography (Fig. 3D) analyses. These analyses demonstrated that the released sugars were essentially all in the form of [3H]galactose-beta 1,4-N-acetylglucosaminitol, the expected product of galactose-labeled O-GlcNAc. Thus, essentially all of the PNGase F-resistant [3H]galactose was linked to O-GlcNAc.

To examine whether we could see changes in the level of O-GlcNAc on proteins in response to nocodazole treatment, we examined the pattern of O-GlcNAc-modified proteins from control and nocodazole-treated cells by SDS-PAGE and fluorography after PNGase F treatment (Fig. 4A). As shown above, a large increase in PNGase F-sensitive terminal GlcNAc residues was induced by the nocodazole treatment. In contrast, the pattern of the PNGase F-resistant species (which represent the O-GlcNAc-labeled species as demonstrated above) did not change dramatically upon nocodazole treatment. Some minor changes could be seen on the gel, but the overall pattern was unchanged. Likewise, the overall total protein pattern was largely unchanged by nocodazole treatment (Fig. 4B). Thus, it appears that nocodazole does not induce major changes in the levels of glycosylation on the more abundant O-GlcNAc-bearing proteins (i.e. those that can be detected in a crude cell lysate) in HT29 cells. We can conclude from these data that global changes in the pattern of O-GlcNAc-modified proteins like those observed by Kearse and Hart (11) in mitogenically activated T-cells are not induced by nocodazole treatment.

Nocodazole Treatment Has Little or No Effect on the Glycosylation of the Nuclear Pore Protein p62 or the Transcription Factor Sp1

To more carefully examine changes in the glycosylation of specific O-GlcNAc-modified proteins other than the keratins, we performed galactosyltransferase labelings of the nuclear pore protein p62 and the transcription factor Sp1 from control and nocodazole-treated cells. Both of these proteins are known to be modified with O-GlcNAc (24-26). The nuclear pore proteins are among the most abundant and heavily O-GlcNAc-modified proteins in mammalian cells (24). Thus, they serve as a good marker of the level of O-GlcNAc modification in a cell. Monoclonal antibody 414, which reacts primarily with p62 and more weakly with other nuclear pore proteins (25), was used to immunoprecipitate the protein from control and nocodazole-treated HT29 cells. The immunoprecipitates were then labeled with galactosyltransferase to detect O-GlcNAc. As can be seen in Fig. 5A, p62 labeled quite well using this technique. The presence or absence of nocodazole had very little effect on the level of its glycosylation. The levels of the p62 protein were monitored by Western blot analysis with monoclonal antibody 414 (Fig. 5B). If anything, this analysis showed that nocodazole induces a slight increase in the amount of the p62 protein. Thus, there does not appear to be an increase in the sugar/protein ratio on p62. We also examined the glycosylation of the transcription factor Sp1 using the same procedure. Sp1 exists as two major molecular mass species in cells (95 and 105 kDa), both of which have been shown to be glycosylated (26). Both species were present in untreated HT29 cells (Fig. 5D), and both appeared to be glycosylated (Fig. 5C). Nocodazole treatment caused an accumulation of the 105-kDa form (Fig. 5, C and D), but the overall level of glycosylation on the protein did not increase. Taken together, these data suggest that the changes seen in glycosylation of the keratins are selective in that similar changes do not occur on all O-GlcNAc-modified proteins in the cell.


Fig. 5. Nocodazole does not induce changes in the glycosylation of nuclear pore proteins or the transcription factor Sp1. HT29 cells were treated with or without nocodazole as described in the legend to Fig. 3, and the cells were lysed in RIPA buffer. Immunoprecipitations of the nuclear pore proteins and Sp1 were performed from extracts containing 1 mg of protein as described under "Experimental Procedures." The control for the nuclear pore antibody (414) was an isotype-matched mouse IgG. The control for the Sp1 antibody (PEP2) was competition with the peptide against which the antibody was raised. The nuclear pore protein (A) and Sp1 (C) immunoprecipitates were labeled with galactosyltransferase. All samples were analyzed by SDS-PAGE and fluorography. The migration positions of Sp1 (both the 95- and 105-kDa species) and of the nuclear pore protein p62 are indicated. Molecular masses (in kilodaltons) of standard proteins are indicated between the panels. The migration position of the IgG heavy chain, which also labels with galactosyltransferase, is indicated. Western blotting of parallel samples using antibodies to nuclear pore proteins (B) and Sp1 (D) was also performed.
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Nocodazole Treatment Has No Effect on the O-GlcNAc Glycosylation Machinery

In addition to examining the level of O-GlcNAc on proteins in nocodazole-treated cells, we assayed for changes in the activity levels of the enzymes responsible for the addition (O-GlcNAc-transferase) or removal (O-GlcNAc'ase) of O-GlcNAc. Changes in the activity of these enzymes would be indicative of a general effect of nocodazole on glycosylation. We have modified the standard assay for both of these enzymes so that they can be detected in crude extracts of ~107 cells (see "Experimental Procedures"). Using these modified assays on extracts of HT29 cells treated for increasing times with nocodazole, we saw no significant changes in activity that correlate with the increase in glycosylation seen on the keratins (Fig. 6, A and B). In addition, since UDP-GlcNAc is a substrate for O-GlcNAc-transferase and could be limiting, we examined the levels of UDP-GlcNAc over the course of nocodazole treatment (Fig. 6C). The technique used for analysis of UDP-GlcNAc was not capable of resolving UDP-GlcNAc from UDP-GalNAc (21). Thus, the data are shown as the total UDP-HexNAc levels. The ratio of UDP-GlcNAc to UDP-GalNAc in a cell is determined by an epimerase capable of interconverting the two, and a relatively constant ratio of ~2:1 (UDP-GlcNAc/UDP-GalNAc) is found in most cells (see "Experimental Procedures"). A dramatic increase in UDP-HexNAc levels was observed during the latter time points of the nocodazole treatment, but these changes occurred much later than the observed increase in O-GlcNAc levels on the keratins. Thus, we could detect no gross changes in the glycosylation machinery responsible for the addition and/or removal of O-GlcNAc from proteins. These data are consistent with the observations discussed above and suggest that the change in glycosylation of the keratins induced by nocodazole is a selective phenomenon.


Fig. 6. Nocodazole does not induce changes in the cellular machinery responsible for the addition or removal of O-GlcNAc from proteins. HT29 cells were treated with nocodazole for the indicated times and extracted as described under "Experimental Procedures." Assays for O-GlcNAc-transferase and O-GlcNAc'ase were performed. Parallel samples were extracted with perchloric acid and used to quantitate nucleotide sugar levels, as described under Experimental Procedures." A, O-GlcNAc-transferase activity; B, O-GlcNAc'ase activity; C, UDP-HexNAc levels.
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DISCUSSION

The modulation of post-translational modifications such as phosphate groups on proteins is well documented. The level of phosphate on proteins is controlled by the relative activity of the enzymes responsible for addition (kinases) and removal (phosphatases) of the phosphate. One implication of such systems is that stimuli that activate or inhibit these enzymes can cause changes in modification of numerous target proteins. For example, stimulation of cAMP-dependent protein kinase with cAMP results in phosphorylation of numerous target proteins. Thus, if nocodazole were a modulator of a system controlling O-GlcNAc levels, one would predict that the levels of glycosylation on numerous proteins would change. Since nocodazole is a microtubule-destabilizing agent that arrests the cell at the G2/M interface of the cell cycle, such an effect could be associated with cell cycle-induced changes. The goal of this report was to test this prediction and to determine whether such a system is operating in HT29 cells with nocodazole as a stimulator.

In this report, we have shown that nocodazole causes a marked increase in the level of O-GlcNAc on keratin 18, as was originally reported by Chou and Omary (12). In contrast, analysis of O-GlcNAc levels on other proteins in the nocodazole-treated cells showed few, if any, changes. Consistent with this observation is the fact that we saw no changes in the glycosylation machinery (O-GlcNAc-transferase, O-GlcNAc'ase, and UDP-GlcNAc levels) induced by nocodazole that would explain the increase in glycosylation of keratin 18. Thus, nocodazole appears to induce an increase in glycosylation of keratin 18 selectively. This implies that the glycosylation machinery is not activated by nocodazole treatment and that the increase in glycosylation of keratin 18 must result from a change in the keratins themselves.

Since nocodazole causes a reorganization of the cytoskeletal components, including the intermediate filaments (12), the simplest explanation for the change in glycosylation of the keratins is that it is related to this reorganization. Omary's group (12, 13) has done extensive work showing that agents that cause reorganization of intermediate filaments in HT29 cells result in dramatic increases in both the glycosylation and phosphorylation of the keratins. In addition to the changes induced by inhibitors that arrest the cell cycle at the G2/M interface (okadaic acid and microtubule-destabilizing agents such nocodazole and colcemid) (12, 13), Omary and co-workers (27) have shown that heat shock causes a rapid increase in the glycosylation and phosphorylation of keratins 8 and 18. These results suggest that the changes in phosphorylation and/or glycosylation are related to the reorganization. Mutation of a major phosphorylation site in keratin 18 does not alter intermediate filament assembly, but it does interfere with filament reorganization of keratins 8 and 18 induced by G2/M arrest in transfected cells (28). Thus, phosphorylation appears to play an active role in filament reorganization in HT29 cells. A similar role for the glycosylation of the keratins is not yet clear. Mutation of the major glycosylation sites of keratin 18 had no effects on filament assembly in transfected cells (29), although the effects of the mutation on reorganization induced by G2/M arrest have not been examined. Recent studies have demonstrated that mutation of a conserved arginine in keratin 18 is sufficient to induce a significant reorganization of intermediate filament structures. The glycosylation and phosphorylation of this mutated keratin 18 are significantly increased compared with the wild-type protein (30). The increase in glycosylation observed on this keratin 18 bearing a point mutation strongly suggests that the change in glycosylation is a result of intermediate filament reorganization, and not a cause. Such a conclusion is consistent with our findings that the increase in glycosylation caused by nocodazole is apparently selective for the keratins. Further work will be needed to conclusively determine the role glycosylation plays in intermediate filament reorganization. Interestingly, not all treatments that cause reorganization of intermediate filaments have the same effects. Rotavirus infection of HT29 cells, which causes intermediate filament reorganization, results in an increase in phosphorylation of keratins 8 and 18, but no change in their glycosylation (27). Thus, the signals that result in changes in glycosylation or phosphorylation of the keratins appear to be distinct.

The most dramatic change in glycosylation induced by nocodazole is the increase in terminal GlcNAc residues on N-glycans. Other workers have shown that short-term treatments with nocodazole (e.g. 1-3 h) have no effect on the movement or glycosylation of proteins through the Golgi apparatus (31). Microtubules are believed to be involved in retrograde transport of proteins from the Golgi apparatus to the endoplasmic reticulum (32), and nocodazole has been used to inhibit this transport. The effects of nocodazole on retrograde transport are also seen after fairly short treatments (<3 h). We see no effect on glycosylation until after 8 h (see Fig. 2A). Thus, the effects we see are apparently not related to the short-term effects of microtubule disassembly, but are more likely related to arrest of the cells at the G2/M interface. Most vesicular traffic in cells, including endoplasmic reticulum to Golgi apparatus and intra-Golgi traffic, has been shown to be interrupted during mitosis (33). The Golgi apparatus fragments, assisting in even distribution of the Golgi apparatus between the two daughter cells. Thus, the accumulation of the terminal GlcNAc residues on N-glycans that we see is most likely a result of the mitosis-induced interruption of medial to trans-Golgi transport. Since galactosyltransferase is a trans-Golgi enzyme (34), lack of medial to trans-Golgi movement would result in an accumulation of unmodified GlcNAc residues. The accumulation we see may suggest that there is a slight time delay between interruption of intra-Golgi transport (medial to trans) and that of endoplasmic reticulum to Golgi transport. The mechanism of Golgi fragmentation, as well as the rationale for why transport stops at mitosis, is not well understood (33). Several workers have suggested that Golgi vesiculation may result from the normal process of vesicular budding from Golgi stacks coupled with an inhibition of vesicle fusion. Thus, vesicles form but are unable to fuse with the next stack. The fact that terminal GlcNAc moieties on N-glycans increase under these conditions may serve as a useful assay to follow this process and allow for a better understanding of the underlying mechanisms.

The results presented in this paper suggest several important factors that must be kept in mind when analyzing changes in the levels of O-GlcNAc on proteins. Galactosyltransferase is still the best method available for analyzing O-GlcNAc levels, and PNGase F digests must be performed on each labeling to assess the contribution of N-glycans to the final pattern. If we had analyzed only the galactosyltransferase-labeled samples from the untreated cells with PNGase F, we would have concluded that >90% of the galactose incorporated was on O-GlcNAc and that PNGase F controls were unnecessary in this cell type. In contrast, the nocodazole treatment caused a dramatic change in galactose labeling of N-glycans. In addition, this study underscores the importance of following protein levels as well as sugar levels before conclusions about changes in glycosylation of any protein can be made. Initial studies with Nonidet P-40 lysates of HT29 cells showed an apparent decrease in glycosylation of some proteins (see Fig. 2). We believe that the decrease has to do with changes in the solubility of proteins induced by the nocodazole treatment of the cells, and not with true changes in the level of glycosylation. Once the change in the levels of the proteins was abrogated by a more efficient extraction technique (e.g. RIPA buffer or 1% SDS), the changes in the glycosylation level disappeared. Thus, in future studies on the levels of O-GlcNAc on proteins, it will be essential to control for labeling of N-glycans with PNGase F and to closely follow the level of the proteins being examined as well as the level of the sugar on them.


FOOTNOTES

*   This work was supported in part by National Institutes of Health Grant GM 48666. Work performed at the Center for Analysis and Synthesis of Macromolecules, State University of New York at Stony Brook, was supported by National Institutes of Health Grant RR02427.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Dagger    Recipient of an American Cancer Society junior faculty research award. To whom correspondence should be addressed. Tel.: 516-632-7336 Fax: 516-632-8575.
1   The abbreviations used are: O-GlcNAc, O-linked N-acetylglucosamine; O-GlcNAc-transferase, UDP-GlcNAc:peptide beta -N-acetylglucosaminyltransferase (9); O-GlcNAc'ase, peptide O-GlcNAc-beta -N-acetylglucosaminidase (10); PNGase F, peptide N-glycosidase F; PAGE, polyacrylamide gel electrophoresis; RIPA, radioimmune precipitation assay; UDP-HexNAc, uridine diphospho-N-acetylhexosamine.

ACKNOWLEDGEMENTS

We thank Scott Busby, Kathleen Grove, Sean Li, Lillian Medina, Daniel Moloney, Dr. Deborah Brown, and Dr. James Trimmer for critical reading of this manuscript and helpful discussions. We also thank Mona Sheth, Scott Busby, and Patrick Reilly for assistance in modifying the assays used for both O-GlcNAc-transferase and O-GlcNAc'ase. Peptide synthesis was performed at the Center for Analysis and Synthesis of Macromolecules and the Center for Biotechnology, State University of New York at Stony Brook.


REFERENCES

  1. Haltiwanger, R. S., Kelly, W. G., Roquemore, E. P., Blomberg, M. A., Dong, L.-Y. D., Kreppel, L., Chou, T.-Y., and Hart, G. W. (1992) Biochem. Soc. Trans. 20, 264-269 [Medline] [Order article via Infotrieve]
  2. Hart, G. W., Kreppel, L. K., Comer, F. I., Arnold, C. S., Snow, D. M., Ye, Z., Cheng, X., DellaManna, D., Caine, D. S., Earles, B. J., Akimoto, Y., Cole, R. N., and Hayes, B. K. (1996) Glycobiology 6, 711-716 [Medline] [Order article via Infotrieve]
  3. Haltiwanger, R. S., Busby, S., Grove, K., Li, S., Mason, D., Medina, L., Moloney, D. J., Philipsberg, G. A., and Scartozzi, R. (1997) Biochem. Biophys. Res. Commun., in press
  4. Holt, G. D., Haltiwanger, R. S., Torres, C.-R., and Hart, G. W. (1987) J. Biol. Chem. 262, 14847-14850 [Abstract/Free Full Text]
  5. Chou, T.-Y., Hart, G. W., and Dang, C. V. (1995) J. Biol. Chem. 270, 18961-18965 [Abstract/Free Full Text]
  6. Kelly, W. G., Dahmus, M. E., and Hart, G. W. (1993) J. Biol. Chem. 268, 10416-10424 [Abstract/Free Full Text]
  7. Chou, C.-F., Smith, A. J., and Omary, M. B. (1992) J. Biol. Chem. 267, 3901-3906 [Abstract/Free Full Text]
  8. Roquemore, E. P., Chevrier, M. R., Cotter, R. J., and Hart, G. W. (1996) Biochemistry 35, 3578-3586 [CrossRef][Medline] [Order article via Infotrieve]
  9. Haltiwanger, R. S., Blomberg, M. A., and Hart, G. W. (1992) J. Biol. Chem. 267, 9005-9013 [Abstract/Free Full Text]
  10. Dong, D. L.-Y., and Hart, G. W. (1994) J. Biol. Chem. 269, 19321-19330 [Abstract/Free Full Text]
  11. Kearse, K. P., and Hart, G. W. (1991) Proc. Natl. Acad. Sci. U. S. A. 88, 1701-1705 [Abstract]
  12. Chou, C.-F., and Omary, M. B. (1993) J. Biol. Chem. 268, 4465-4472 [Abstract/Free Full Text]
  13. Chou, C.-F., and Omary, M. B. (1994) J. Cell Sci. 107, 1833-1843 [Abstract/Free Full Text]
  14. Roquemore, E. P., Chou, T.-Y., and Hart, G. W. (1994) Methods Enzymol. 230, 443-460 [Medline] [Order article via Infotrieve]
  15. Tarentino, A. L., Gomez, C. M., and Plummer, T. H., Jr. (1985) Biochemistry 24, 4665-4671 [Medline] [Order article via Infotrieve]
  16. Chou, C. F., and Omary, M. B. (1991) FEBS Lett. 282, 200-204 [CrossRef][Medline] [Order article via Infotrieve]
  17. Haltiwanger, R. S., Holt, G. D., and Hart, G. W. (1990) J. Biol. Chem. 265, 2563-2568 [Abstract/Free Full Text]
  18. Harlow, E., and Lane, D. P. (1988) Antibodies: A Laboratory Manual, pp. 421-510, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
  19. Lin, A. I., Philipsberg, G. A., and Haltiwanger, R. S. (1994) Glycobiology 4, 895-901 [Abstract]
  20. Kobata, A. (1994) Methods Enzymol. 230, 200-208 [Medline] [Order article via Infotrieve]
  21. Wice, B. M., Trugnan, G., Pinto, M., Rousset, M., Chevalier, G., Dussaulx, E., Lacroix, B., and Zweibaum, A. (1985) J. Biol. Chem. 260, 139-146 [Abstract/Free Full Text]
  22. Hardy, M. R., and Townsend, R. R. (1994) Methods Enzymol. 230, 208-225 [Medline] [Order article via Infotrieve]
  23. Laemmli, U. K. (1970) Nature 227, 680-685 [Medline] [Order article via Infotrieve]
  24. Holt, G. D., Snow, C. M., Senior, A., Haltiwanger, R. S., Gerace, L., and Hart, G. W. (1987) J. Cell Biol. 104, 1157-1164 [Abstract]
  25. Davis, L. I., and Blobel, G. (1987) Proc. Natl. Acad. Sci. U. S. A. 84, 7552-7556 [Abstract]
  26. Jackson, S. P., and Tjian, R. (1988) Cell 55, 125-133 [Medline] [Order article via Infotrieve]
  27. Liao, J., Lowthert, L. A., and Omary, M. B. (1995) Exp. Cell Res. 219, 348-357 [CrossRef][Medline] [Order article via Infotrieve]
  28. Ku, N.-O., and Omary, M. B. (1994) J. Cell Biol. 127, 161-171 [Abstract]
  29. Ku, N.-O., and Omary, M. B. (1995) J. Biol. Chem. 270, 11820-11827 [Abstract/Free Full Text]
  30. Ku, N.-O., Michie, S., Oshima, R. G., and Omary, M. B. (1995) J. Cell Biol. 131, 1303-1314 [Abstract]
  31. Stults, N. L., Fechheimer, M., and Cummings, R. D. (1989) J. Biol. Chem. 264, 19956-19966 [Abstract/Free Full Text]
  32. Lippincott-Schwartz, J., Donaldson, J. G., Schweizer, A., Berger, E. G., Hauri, H.-P., Yuan, L. C., and Klausner, R. D. (1990) Cell 60, 821-836 [Medline] [Order article via Infotrieve]
  33. Warren, G. (1993) Annu. Rev. Biochem. 62, 323-348 [CrossRef][Medline] [Order article via Infotrieve]
  34. Kornfeld, R., and Kornfeld, S. (1985) Annu. Rev. Biochem. 54, 631-664 [CrossRef][Medline] [Order article via Infotrieve]

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