(Received for publication, April 9, 1997)
From the Departments of Pharmacology and Chemistry
and Biochemistry, University of California at San Diego,
La Jolla, California 92093-0640
Mature protein kinase C is phosphorylated at a
conserved carboxyl-terminal motif that contains a Ser (or Thr)
bracketed by two hydrophobic residues; in protein kinase C II, this
residue is Ser-660 (Keranen, L. M., Dutil, E. M., and Newton, A. C. (1995) Curr. Biol. 5, 1394-1403). This contribution
examines how negative charge at this position regulates the function of
protein kinase C. Specifically, Ser-660 in protein kinase C
II was
mutated to Ala or Glu and the enzyme's stability, membrane
interaction, Ca2+ regulation, and kinetic parameters were
compared with those of wild-type protein phosphorylated at residue 660. Negative charge at this position had no significant effect on the
enzyme's diacylglycerol-stimulated membrane interaction nor the
conformational change accompanying membrane binding. In contrast,
phosphate caused a 10-fold increase in the enzyme's affinity for
Ca2+ and a comparable increase in its affinity for
phosphatidylserine, two interactions that are mediated by the C2
domain. Negative charge also increased the protein's thermal stability
and decreased its Km for ATP and peptide substrate.
These data indicate that phosphorylation at the extreme carboxyl
terminus of protein kinase C structures the active site so that it
binds ATP and substrate with higher affinity and structures
determinants in the regulatory region enabling higher affinity binding
of Ca2+. The motif surrounding Ser-660 in protein kinase C
II is found in a number of other kinases, suggesting interactions
promoted by phosphorylation of the carboxyl terminus may provide a
general mechanism for stabilizing kinase structure.
Phosphorylation is a widely used mechanism for reversibly regulating protein structure and function. Conformational or electrostatic changes promoted by phosphorylation modulate the enzymatic activity and macromolecular interactions of a plethora of cellular proteins (1). Since the discovery 4 decades ago that phosphorylation activates phosphorylase kinase (2), it has been clearly established that phosphorylation serves as a general mechanism for regulating kinase function (1, 3, 4). Best characterized is the requirement for negative charge on the activation loop of kinases that renders the kinase core competent for catalysis (3, 4).
Phosphorylation has recently been shown to play an essential role in regulating the protein kinase Cs (5, 6). These enzymes transduce the myriad of signals promoting phospholipid hydrolysis (7). They are recruited to membranes upon the production of diacylglycerol and, for the conventional isoforms, increased Ca2+ concentrations. Binding of these cofactors results in a conformational change that removes an autoinhibitory (pseudosubstrate) domain from the active site, thus promoting substrate binding and phosphorylation (8). It was recently established that protein kinase C is multiply phosphorylated in vivo and that these phosphorylations are required to process newly synthesized protein kinase C into the mature, cofactor-responsive enzyme that has been extensively studied over the past 2 decades (5, 6).
Mass spectrometric analysis established that the protein kinase C
present in the detergent-soluble fraction of cell or tissue extracts is
phosphorylated at three conserved positions, with non-phosphorylated or
partially phosphorylated forms partitioning exclusively in the
detergent-insoluble fraction (5). In protein kinase C II, these
phosphorylation sites are Thr-500, Thr-641, and Ser-660 (5, 6). Based
on distinct electrophoretic mobility shifts resulting from these
phosphorylations and the use of antibodies selective for
dephosphorylated Ser-660, the order of phosphorylations was established
to be Thr-500, followed by Thr-641, followed by Ser-660 (5). Each of
these phosphorylatable residues has an analogue in other protein
kinases: Thr-500 aligns with the activation loop phosphorylatable
residue found in many protein kinases (including Thr-197 in protein
kinase A (4)), Thr-641 has an analogous site on protein kinase A (S338)
(9), and Ser-660 is part of a hydrophobic phosphorylation motif present
in diverse kinases such as S6 kinase and protein kinase B (Akt kinase)
that has the consensus sequence FXXF(S/T)(F/Y)
(10, 11).
The first phosphorylation event, occurring on the activation loop,
appears to be catalyzed by an unidentified protein kinase C kinase (5,
12), whereas the two carboxyl-terminal phosphorylations are
autophosphorylations (13-15). Phosphorylation at the activation loop
is required to initiate the processing of inactive protein kinase C to
the cofactor-activable, mature form (12, 16, 17). However, once the
mature, carboxyl-terminal phosphorylated enzyme is formed, the
requirement for negative charge at the activation loop is relieved (5).
The second phosphorylation event, occurring on the first
carboxyl-terminal site, Thr-641, has been proposed to be critical to
the catalytic function of protein kinase C (5), to allow the release of
protein kinase C from the detergent-insoluble fraction of cells (18),
and to stabilize protein kinase C (19). Although most of the
phosphorylation sites are conserved throughout the protein kinase C
family, some isozymes contain a Glu in place of a phosphorylatable
residue in the hydrophobic motif (5). Furthermore, protein kinase C
, which has the most divergent activation loop sequence, does not
require phosphorylation of the residue corresponding to Thr-500 in
protein kinase C
II for the formation of mature, activable
enzyme (20).
This contribution explores the role of the final phosphorylation event,
that at position Ser-660, in regulating protein kinase C II.
Specifically, Ser-660 was mutated to Glu (present in two other isozymes
(5)) to mimic the effect of phosphate or Ala to mimic the neutral,
non-phosphorylated residue. Kinetic and binding analyses reveal that
negative charge at this position markedly increases the stability of
the enzyme, its affinity for substrate, and its affinity for
Ca2+. These data reveal that phosphorylation at this
carboxyl-terminal hydrophobic motif plays a role in structuring both
the kinase core and determinants in the regulatory domain.
Bovine brain L--phosphatidylserine,
1-palmitoyl-2-oleoyl-phosphatidylcholine,
1-palmitoyl-2-oleoyl-phosphatidylserine, and sn-1,2-dioleoylglycerol were purchased from Avanti Polar
Lipids, Inc. Dithiothreitol (DTT),1 HEPES,
EGTA, trypsin (type XIII from bovine pancreas 10 units µg
1), and ATP were from Sigma.
[
-32P]ATP (3000 Ci mmol
1) and
[3H]dipalmitoyl phosphatidylcholine were from NEN Life
Science Products, and calcium chloride (analytical grade) was from J. T. Baker, Inc. Peroxidase-conjugated goat anti-rabbit antibodies and
bovine serum albumin were obtained from Boehringer Mannheim.
Chemiluminescence SuperSignal substrates were from Pierce. Lipofectin
reagent was purchased from Life Technologies, Inc. and BaculoGold DNA
was from Pharmingen. A protein kinase C-selective peptide
(Ac-FKKSFKL-NH2; (21)) was synthesized by the Indiana
University Biochemistry Biotechnology Facility. All other chemicals
were reagent grade. A polyclonal antibody against the bacterially
expressed catalytic domain of protein kinase C
II was a gift from
Drs. Andrew Flint and Daniel E. Koshland, Jr.
Expression vectors encoding the cDNA
sequence of protein kinase C II with mutation of Ser-660 to Ala or
Glu were made by polymerase chain reaction using protein kinase C
II
in the pBluescript vector (pbluePKC) as the template. The sense primer
used for both mutants was GGAGCATGCATTTTTCCG and contains an
NsiI restriction site. Antisense primers corresponding to
the sequence around the codon for Ser-660 and containing the necessary
nucleic acid changes to encode the desired mutation were
CAGAGTTAACAAAGGCAAATCCTTCGAATTCTG (S660A mutation) and
CAGAGTTAACAAACTCAAATCCTTCGAATTCTG (S660E mutation). These
antisense primers contain an HpaI restriction site. The
polymerase chain reaction product and the pbluePKC template were
digested with NsiI and HpaI (unique sites in
pbluePKC), and the products were gel-purified and ligated together. The
mutant protein kinase C gene was then subcloned into the pVL1393
(Invitrogen) baculovirus transfer vector using XbaI and
SmaI (see below). The sequence of all mutants was verified
by DNA sequencing.
Sf21
insect cells were cotransfected with the baculoviral transfer vectors
encoding the protein kinase C mutants and linearized wild-type
baculovirus DNA (BaculoGold, Pharmingen) by liposome-mediated transfection. Isolated recombinant baculovirus was obtained by plaque
purification and amplified by two rounds of propagation in insect cells
as described in a manual from Pharmingen for the BaculoGold expression
system (Pharmingen, San Diego). Sf21 insect cells were then infected
with high titer (1 × 108 plaque-forming units
ml1) baculovirus encoding wild-type protein kinase C
II or its mutants. The cells were harvested after 3 days at 27 °C
and lysed by homogenization in buffer containing 50 mM
HEPES (pH 7.4), 0.2% Triton X-100, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 85 µM
leupeptin, 2 mM benzamidine, and 0.2 mM
phenylmethanesulfonyl fluoride (lysis buffer). A portion of the lysate
was retained, and the remainder was centrifuged at 100,000 × g for 20 min at 4 °C (TLA 120.2, Beckman). The pellet was
resuspended in lysis buffer, and lysate, supernatant
(detergent-soluble), and pellet (detergent-insoluble) fractions were
diluted 2-fold in glycerol and stored at
20 °C. All experiments
were performed using the high speed, detergent-soluble
supernatants.
Aliquots of cell lysate,
detergent-soluble supernatant, or detergent-insoluble pellet from Sf21
cells expressing wild-type protein kinase C II or the Ser-660
mutants were analyzed by SDS-polyacrylamide gel electrophoresis (7%
polyacrylamide unless otherwise stated). The proteins were then
transferred to polyvinylidene difluoride (Immobilon-P, Millipore) and
probed with antibodies to the catalytic domain of protein kinase C
II and peroxidase-conjugated secondary antibodies. Labeling was
detected using chemiluminescence.
Protein kinase C activity in 1-3
µl of detergent-soluble fractions was assayed by measuring the rate
of phosphorylation of a synthetic peptide in the presence or absence of
brain phosphatidylserine, diacylglycerol, and Ca2+, as
described (22). The reaction mixture contained 50 µM (or indicated amounts) protein kinase C-selective peptide in 20 mM HEPES (pH 7.5 at 30 °C), 1 mM DTT, 100 µM [-32P]ATP (unless otherwise
indicated), 5 mM MgCl2, and either 0.5 mM Ca2+ and lipid (sonicated dispersion of
brain phosphatidylserine (140 µM) and diacylglycerol (3.8 µM), prepared as described (22)) or 0.5 mM
EGTA in a final volume of 80 µl. Samples were incubated at 30 °C
for 4-6 min and quenched by the addition of 25 µl of a solution
containing 0.1 M ATP and 0.1 M EDTA (pH 8-9).
Aliquots (85 µl) were spotted on P81 ion-exchange chromatography
paper and washed four times with 0.4% (v/v) phosphoric acid, followed by a 95% ethanol rinse, and 32P incorporation was detected
by liquid scintillation counting in 5 ml of scintillation fluid
(Biosafe II, Research Products International Corp.).
Sucrose-loaded large
unilamellar vesicles composed of 40 mol % 1-palmitoyl-2-oleoyl-phosphatidylserine, 55 or 60 mol % 1-palmitoyl-2-oleoyl-phosphatidylcholine, and 0 or 5 mol % diacylglycerol and containing trace [3H]dipalmitoyl
phosphatidylcholine were prepared by extrusion as described (23). Stock
phospholipid concentrations were determined by phosphate analysis (24).
Concentrations of lipids recovered after extrusion were calculated from
radioactivity. The interaction of protein kinase C with sucrose-loaded
vesicles was measured as described by Rebecchi et al. (25)
and adopted for protein kinase C (26). Briefly, protein kinase C
(15-20 µl of detergent-soluble supernatant containing wild-type or
mutant protein kinase C) was incubated with vesicles (75 µM total lipid) in the presence of 100 nM to
1 mM Ca2+, in buffer containing 20 mM HEPES (pH 7.5), 0.3 mg ml1 bovine serum
albumin, and 100 mM KCl for 5 min at 22 °C.
Vesicle-bound enzyme was separated from free enzyme by centrifugation
of the vesicle/enzyme mixture at 100,000 × g for 30 min at 25 °C. Aliquots from the supernatant and pellet of the
binding experiment were assayed under identical conditions (1-2 µl
of detergent-soluble supernatant per assay), and the vesicle-associated
kinase activity was calculated as described (23).
Protein kinase C's sensitivity to trypsin was
determined by incubating the Sf21 cell supernatants containing 10-20
ng of wild-type or mutant protein kinase Cs in a total volume of 90 µl (containing 20 mM HEPES, 0.3 mM
Ca2+, with or without brain phosphatidylserine (250 µM) and diacylglycerol (7 µM)) in the
presence of 0-20 units ml1 trypsin, as indicated in the
legend to Fig. 5. Proteolysis was carried out for 10 min at 30 °C
and stopped by addition of 30 µl of SDS loading buffer. Samples were
analyzed by SDS-polyacrylamide gel electrophoresis (9% polyacrylamide)
and followed by Western blot analysis using antibodies that recognize
the catalytic domain of protein kinase C
II.
Free Calcium Determinations
Concentrations of free Ca2+ were calculated using a program provided by Dr. Claude Klee (27) that takes into account pH, Ca2+, Mg2+, K+, Na+, EGTA, EDTA, and ATP concentrations.
Data AnalysisThe dependence of protein kinase C's membrane binding on Ca2+ concentration was analyzed by a nonlinear least-squares fit to the Hill equation using the program Sigma Plot. The dependence of protein kinase C activity on ATP or substrate concentrations was analyzed by Michaelis-Menten kinetics using the program Sigma Plot. Apparent membrane association constraints were calculated by determining the ratio of bound protein kinase C:free protein kinase C divided by the total lipid concentration, as described (26).
Wild-type protein kinase C and the two carboxyl-terminal
phosphorylation site mutants, S660A and S660E, were expressed in insect
cells to characterize the effect of charge at position 660 on the
biochemical properties of protein kinase C. The Western blot in Fig.
1 shows that all three proteins were expressed at comparable levels in Sf21 cells; the antibody used labels
phosphorylated and dephosphorylated protein kinase C equally (5).
Lysate of cells expressing wild-type protein kinase C (L) contained a
major band migrating with an apparent molecular mass of 80 kDa
(double asterisk), and a minor faster-migrating form with an
apparent mass of 76 kDa (dash). The upper band partitioned
in the detergent-soluble fraction and the lower band partitioned in the
detergent-insoluble fraction. Mass spectrometric analysis previously
established that the upper band is quantitatively phosphorylated at the
two carboxyl-terminal phosphorylation sites, Thr-641 and Ser-660, and
that the lower band is not phosphorylated at either of these positions
(5) (an intermediate-migrating band, not labeled detectably in
wild-type lysate in Fig. 1, contains phosphate at Thr-641 but not
Ser-660). We have also shown previously (5) that phosphorylation at
each carboxyl-terminal position results in a distinct electrophoretic shift. In contrast, phosphorylation of Thr-500, the first
phosphorylation event, does not result in a change in protein kinase
C's electrophoretic mobility; in addition, only about half of the
mature protein kinase C (upper-migrating band) is phosphorylated at
this position (5).
The S660A mutant and S660E mutant in cell lysates (L) resolved into two main bands. For the S660A mutant, the upper main band migrated with an apparent mass of 78 kDa (indicated by single asterisk in Fig. 1), just below that of the fully phosphorylated (80 kDa) wild-type enzyme. This is the position where protein phosphorylated at Thr-641 but not Ser-660 migrates (5), indicating that substitution of Ser-660 with Ala mimics the electrophoretic mobility of wild-type protein with no phosphate at position 660. The lowest S660A band comigrated with the lowest band for wild type (dash), again indicating the equivalence of dephosphorylated Ser and Ala at position 660 in affecting the mobility of protein kinase C. For the S660E mutant, the upper band comigrated with the upper band of wild-type protein kinase C (double asterisk), revealing that Glu mimics phosphate in inducing an electrophoretic mobility shift. Consistent with this, the lowest S660E band migrated with an intermediate mobility (single asterisk). Thus, negative charge at position 660 decreases the electrophoretic mobility of protein kinase C, with Glu or phosphate causing the same mobility change. Curiously, a faintly stained band above the mature protein kinase C was apparent in Western blots of S660A and S660E, suggesting the possibility of additional phosphorylation on a small fraction of the mutant protein kinase C population.
The slower-migrating band for both mutants was preferentially, but not exclusively, localized in the detergent-soluble fraction (S). In particular, half of the slower-migrating (mature) band of S660A partitioned in the detergent-soluble fraction and half partitioned in the detergent-insoluble fraction (P). This contrasted with the slowest-migrating (mature) form of wild-type protein kinase C which partitioned almost exclusively in the detergent-soluble fraction. Thus, Ala at position 660 decreased the solubility of mature protein kinase C. As observed for wild-type enzyme, the faster migrating form of S660A, containing no phosphate at Thr-641, partitioned almost entirely in the detergent-insoluble fraction. A fraction of the mature S660E also partitioned in the detergent-insoluble fraction (P). However, in marked contrast to both wild-type enzyme and the S660A mutant, a significant fraction (65%) of the fastest migrating band of S660E partitioned in the detergent-soluble fraction. This indicates that protein with charge at Ser-660, but not Thr-641 (i.e. faster migrating S660E band), has an increased solubility compared with non-phosphorylated wild-type enzyme; conversely, protein with negative charge at Thr-641 but not Ser-660 (i.e. slower migrating S660A band), has a decreased solubility compared with fully phosphorylated wild-type enzyme. Lysis and fractionation in the absence of detergent revealed similar partitioning of the mature wild-type protein kinase C in the soluble fraction, revealing that it is localized in the cytosol (data not shown). Thus, negative charge at position 660 promotes the release of protein kinase C from the detergent-insoluble fraction into the cytosol.
In the following experiments, the biochemical properties of wild-type and mutant protein kinase Cs recovered in the detergent-soluble fraction of cell lysates were characterized. Importantly, the wild-type protein kinase C in these extracts was quantitatively phosphorylated on Ser-660, as assessed by its migration as a single 80-kDa band on SDS-polyacrylamide gel electrophoresis and as confirmed previously by mass spectrometry (5).
Fig. 2 shows that the baculovirus-expressed mutants
displayed lipid-dependent activity when assayed under
standard conditions (see "Materials and Methods"); however, the
specific activity of the mutants was reduced relative to wild type.
Under these assay conditions, S660A phosphorylated a synthetic peptide
based on the myristoylated alanine-rich C kinase substrate (MARCKS) protein (protein kinase C-selective peptide (21)) at approximately 25%
of the rate of wild-type enzyme, and S660E phosphorylated the peptide
at approximately 61% of the rate of wild-type enzyme.
To explore whether the reduced activity of the mutants arose because of
decreased thermal stability, activity was examined as a function of
incubation time in the presence or absence of lipid cofactors. Fig.
3A shows that wild-type protein kinase C () was relatively stable when incubated at 22 °C, losing only 20% of its activity after a 3-h incubation; no significant difference was observed in the presence or absence of lipid cofactors. In contrast, S660A (
) was thermally labile, losing half of its activity after a 20-min incubation in the presence of lipid (A) and
60 min in the absence of lipid (B). S660E (
) displayed
intermediate stability, losing half of its activity after approximately
90 min incubation in the presence of lipid (A); as with
S660A, the rate of activity loss of S660E was approximately three times
slower in the absence of lipid (B). This loss of activity
did not result from proteolysis, as assessed by analysis on
polyacrylamide gels, nor did it result from dephosphorylation:
inclusion of microcystin in incubation mixtures had no effect on the
loss of activity (data not shown). Thus, Ala at position 660 markedly
decreased the thermal stability of protein kinase C, with Glu at that
position partially protecting against destabilization. In addition, the
active, lipid-bound conformation of protein kinase C was significantly
more thermally labile than the inactive, unbound conformation.
To address whether the 660 mutants were impaired catalytically, or
whether the decreased activity observed in Fig. 2 arose because the
enzyme was so labile, activity was measured under conditions promoting
protein stability: in the presence of increasing concentrations of
glycerol. Fig. 4 shows that the specific activity of
both mutants approached that of wild-type as glycerol concentrations were raised. Specifically, inclusion of 20% glycerol in the reaction mixture increased the specific activity of S660A and S660E to 70 ± 10 and 90 ± 20%, respectively, of the wild-type specific activity assayed under the same conditions. Similarly, the specific activities of the mutants were closer to that of wild-type when assayed
on ice (data not shown). Thus, the decreased activity in Fig. 2 arises
because of instability of the mutants rather than impaired
catalysis.
We next tested whether the mutants underwent the same conformational
change upon membrane binding as wild-type protein kinase C. The hinge
separating the regulatory and catalytic moieties of protein kinase C is
sensitive to trypsin, and this sensitivity increases at least 10-fold
upon membrane binding (28).2 Fig.
5A shows that when wild-type, S660A, or S660E
enzymes were incubated in the presence of lipid, 0.2 units
ml1 trypsin resulted in proteolysis of most of the native
enzyme and the appearance of the approximately 50-kDa catalytic domain fragment (lane 3); no intact protein kinase C was apparent
after incubation with 2 units ml
1 trypsin (lane
4). In the absence of lipid, higher concentrations of trypsin were
required to observe similar proteolysis; intact protein kinase C was
still apparent after incubation with 2 units ml
1 trypsin
(lane 9) but was not apparent after incubation with 20 units
ml
1 trypsin (lane 10). Thus, the hinge of the
S660A and S660E mutants undergoes the same membrane
binding-dependent exposure as the hinge of protein
phosphorylated at Ser-660. One difference between the mutants and
wild-type protein kinase C was, however, noted: the cleaved catalytic
domain was considerably more sensitive to further proteolysis for the
mutants compared with wild-type enzyme. Although the cleaved catalytic
fragment of wild-type protein kinase C was relatively resistant to
further proteolysis (see Fig. 5A, lanes 3-5), the catalytic
fragment of both mutants was rapidly degraded (Fig. 5, B and
C, lanes 3-5). In summary, the hinge of both mutants became
exposed in the presence of phosphatidylserine and diacylglycerol,
consistent with undergoing the same conformational change as wild-type
enzyme upon membrane binding. However, the proteolytic sensitivity of
the cleaved catalytic domain of the mutants was increased, indicating
that phosphate on Ser-660 stabilizes the kinase core.
The effect of mutation of Ser-660 on protein kinase C's affinity for
membranes was examined in Fig. 6. Wild-type, S660A, and S660E bound phosphatidylcholine vesicles containing 5 mol % diacylglycerol and 40 mol % phosphatidylserine to comparable levels
(Fig. 6, solid columns); analysis of the binding data in
Fig. 6 revealed apparent membrane association constants on the order of
105 M1 for all three protein
kinase Cs. In the absence of diacylglycerol, membrane binding was
significantly decreased for wild-type protein kinase C (open
columns); analysis of data revealed a 30-fold drop in the apparent
membrane association constant, consistent with previous reports (26,
29, 30). This decrease was even more pronounced for the two mutants;
these displayed an approximately 250-fold drop in membrane affinity in
the absence of diacylglycerol. Thus, mutation of Ser-660 to Ala or Glu
caused an approximately 10-fold decrease in protein kinase C's low
affinity membrane interaction that occurs in the absence of
diacylglycerol but did not alter the biologically relevant high
affinity interaction that is induced by diacylglycerol.
Fig. 7 shows that mutation of Ser-660 to Ala caused a
marked decrease in protein kinase C's affinity for Ca2+.
Specifically, the concentration of Ca2+ required for
half-maximal binding to membranes was 7-fold higher for S660A (1.8 ± 0.3 µM) compared with wild-type (0.25 ± 0.03 µM) protein kinase C (Fig. 7A). Glu partially
mimicked the effect of phosphate in increasing protein kinase C's
Ca2+ affinity, with half-maximal binding requiring
0.67 ± 0.05 µM Ca2+. Similarly, the
concentration of Ca2+ eliciting half-maximal activation was
higher for the S660A mutant compared with wild-type enzyme (Fig.
7B). These data reveal that the negative charge on the
carboxyl terminus of protein kinase C contributes to the interaction of
protein kinase C with Ca2+.
The data in Fig. 7B reveal that the Ser-660 mutants had comparable activity to wild-type enzyme in this experiment. In addition to the increased activity promoted by having saturating concentrations of peptide substrate, we have found that the mutants are considerably more active when bound to the large unilamellar vesicles used in the assay in Fig. 7. Thus, binding to membranes containing 40 mol % phosphatidylserine and 5 mol % diacylglycerol stabilizes the Ser-660 mutants; this stabilization is not observed when protein kinase C interacts with the multilamellar phosphatidylserine in the sonicated lipid dispersion used in standard assays (e.g. Fig. 2).
The effect of mutation of Ser-660 on protein kinase C's
Km for ATP was explored in Fig. 8.
Analysis of data from six separate experiments revealed that mutation
of Ser-660 to Ala caused a 3-fold increase in the Km
of the enzyme for ATP, from 37 ± 1 to 100 ± 10 µM. In contrast, mutation of this residue to Glu had no
significant effect on the Km (44 ± 3 µM). The Km for ATP for all three
proteins was the same whether measured in the presence of 50 µM substrate (Fig. 8) or 500 µM substrate
(data not shown). The Km for peptide substrate was
also increased upon removal of the negative charge at Ser-660 (Fig.
9). Compilation of data from six separate experiments revealed that mutation of Ser-660 to Ala resulted in a 5-fold increase
in the Km for peptide substrate (280 ± 20 µM for S660A compared with 57 ± 3 µM
for wild-type), whereas mutation to Glu had no significant effect on
the Km for substrate (45 ± 3 µM). Thus, negative charge at position 660 increases the apparent binding of both ATP and peptide substrate, either by increasing the enzyme's affinity for these substrates or by increasing the catalytic rate constant.
The foregoing studies reveal that phosphorylation at the conserved
hydrophobic motif, FXXF(S/T)(F/Y), on protein
kinase C's carboxyl terminus affects both the kinase core and
regulatory domain of protein kinase C. Specifically, phosphate at
position 660 in protein kinase C II increases the thermal stability
of the kinase, increases the proteolytic stability of the kinase core,
and increases the enzyme's apparent affinity for ATP and peptide
substrate. In addition to these changes involving the kinase domain,
phosphate at Ser-660 increases the enzyme's affinity for
Ca2+ by almost an order of magnitude. It also increases the
enzyme's affinity for phosphatidylserine but does not alter the
enzyme's high affinity membrane interaction that is induced by binding diacylglycerol. Thus, phosphorylation at this conserved motif appears
to play a key role in structuring protein kinase C for higher affinity
binding of substrates to the active site, for higher affinity binding
of Ca2+ and phosphatidylserine to determinants in the
regulatory region, and for increased stability.
For most parameters examined, Glu was an effective mimic of phosphate. However, for some parameters, the S660E mutant displayed properties intermediate between those of the S660A mutant and the phosphorylated wild-type. Thus, the single negative charge of the carboxylate, and perhaps distance and orientational constraints, may have decreased the strength of stabilizing interactions normally formed by the phosphorylated carboxyl terminus.
Negative Charge at Position Ser-660 Alters Protein Kinase C's Electrophoretic MobilityAnalysis of the Ser-660 mutants revealed that the negative charge of Glu mimics the effect of phosphate in decreasing the electrophoretic mobility of protein kinase C. The precursor (non-phosphorylated) S660A in the detergent-insoluble fraction of cells comigrates with the wild-type precursor, whereas the S660E precursor migrates with a slower mobility, mimicking one phosphorylation event. Similarly, the mature S660A (in this case phosphorylated at Thr-500 (no effect on electrophoretic mobility) and Thr-641, with no modification possible at Ser-660) migrates faster than mature wild-type protein kinase C, presumably because there is no negative charge at position 660. Consistent with this, mature S660E comigrates with mature wild-type protein kinase C, presumably because S660E and wild-type have negative charge at position 660. Using selective dephosphorylation and antibodies selective for dephosphorylated Ser-660, we showed previously that phosphorylation of Ser-660 causes a decrease in protein kinase C's electrophoretic mobility. The present study confirms that one of the mobility shifts observed during the processing of protein kinase C arises from a negative charge on residue 660.
Negative Charge on Ser-660 Affects Protein Kinase C's PartitioningFractionation studies revealed that negative charge at position 660 promotes the partitioning of protein kinase C in the detergent-soluble fraction of cells. In particular, a fraction of the precursor S660E (lowermost band, consistent with no phosphorylation of Thr-641) partitioned in the detergent-soluble fraction, revealing increased solubility as a result of negative charge at position 660. Conversely, a fraction of the mature S660A (uppermost band, consistent with phosphorylation of Thr-500 and Thr-641) partitioned in the detergent-insoluble fraction, indicating decreased solubility. These results are consistent with the final phosphorylation event, phosphorylation of Ser-660, regulating the release of protein kinase C from a cytoskeletal anchorage into the cytosol.
Pulse-chase experiments by Fabbro and coworkers (31) showed that
protein kinase C is initially associated with the
detergent-insoluble cell fraction as a faster migrating,
dephosphorylated form and is chased into a slower migrating form that
partitions in the detergent-soluble fraction. However, Parker and
coworkers (32) have recently suggested that newly synthesized
(non-phosphorylated) protein kinase C localizes to the cytosol where it
is phosphorylated, and subsequent dephosphorylation of mature enzyme
results in association with the detergent-insoluble fraction.
Consistent with the former finding, mutation of the first
carboxyl-terminal phosphorylation site to Ala in protein kinase C
I
(Thr-642, corresponding to Thr-641 in protein kinase C
II) traps
precursor kinase in the detergent-insoluble fraction (18). A similar
result is observed when all potential phosphorylation sites around
Thr-641 are mutated in protein kinase C
II; mutation to Ala of
Thr-634, Thr-641, and Ser-654 (absent in protein kinase C
I) traps
precursor enzyme as a faster migrating form in the detergent-insoluble
fraction. Mutation of only Thr-634 and Thr-641 results in a
compensatory phosphorylation as evidenced by an additional
electrophoretic mobility shift and phosphatase sensitivity
experiments3; this compensatory
phosphorylation could account for a report showing that mutation of the
corresponding Thr-638 to Ala does not significantly affect the function
of protein kinase C
and results in mature protein with the same
electrophoretic mobility as wild-type enzyme (19). Although it is
possible that these carboxyl-terminal mutations generate an insoluble
enzyme because it is not properly folded, the increased partitioning of
partially phosphorylated S660E in the detergent-soluble fraction
described in this contribution is consistent with this final
phosphorylation event releasing protein kinase C into the cytosol.
Kinetic analyses revealed that negative charge at position 660 has a marked effect on parameters relating to the structure of the enzyme. That is, negative charge provided by phosphate or Glu at this position decreased the enzyme's Km for ATP and peptide substrate, suggesting that it participates in structuring the active site. Further evidence that phosphorylation of Ser-660 structures the kinase core derives from the finding that mutation of Ser-660 increases the proteolytic sensitivity of the kinase domain, as well as decreasing the thermal stability of the intact enzyme. It is noteworthy that Glu was a poor mimic of phosphate in stabilizing the cleaved catalytic domain from further proteolysis; Glu did, however, partially protect protein kinase C from thermal denaturation.
In the case of protein kinase A, phosphorylation of a carboxyl-terminal
residue, Ser-338 (analogous to Thr-641 in protein kinase C II), also
plays a key role in structuring the catalytic subunit. Determination of
the crystal structure revealed that phosphate at this position anchors
the carboxyl terminus to the top of the ATP-binding lobe of the kinase,
maintaining it away from the active site (33). Mutation of this Ser to
Ala, and to a lesser extent Glu, markedly decreases the enzyme's
stability (9). By analogy, the corresponding phosphorylated position in
protein kinase C
II (Thr-641), perhaps assisted by the phosphate on
Ser-660, may anchor protein kinase C's carboxyl terminus away from the
active site (see Fig. 10). For protein kinase C,
autophosphorylation of these residues appears to be intramolecular (13,
14), indicating that prior to phosphorylation the carboxyl terminus
accesses, or possibly binds, the active site. Thus, a prime function of carboxyl-terminal phosphorylation of protein kinases, in general, may
be to stabilize the kinase core by tethering the carboxyl terminus away
from the active site.
Curiously, treatment of mature protein kinase C with protein phosphatase 2A dephosphorylates Ser-660 (and Thr-500) to yield a protein with phosphate only on Thr-641 that is fully active under standard assay conditions (15). The finding that the mature S660A mutant (i.e. phosphorylated on Thr-641) is so thermally labile that only partial activity is observed under standard assay conditions suggests that mature protein that has been dephosphorylated on Ser-660 is not entirely equivalent to protein that has never had negative charge at Ser-660. Possibly tethering of the carboxyl terminus proposed above requires phosphorylation at both carboxyl-terminal positions, and once tethered, dephosphorylation of Ser-660 does not have as significant an effect on the interaction as having no charge at that position during the maturation. It should be noted, however, that wild-type enzyme dephosphorylated at Ser-660 is unable to re-autophosphorylate when lipid is presented in the form of Triton X-100 mixed micelles (15), consistent with the reduced affinity for cofactors reported for the S660A mutant in this contribution.
Negative Charge on Ser-660 Regulates Ca2+ Binding SiteA surprising finding from this research was that
phosphorylation of Ser-660 affects determinants in the regulatory
region of protein kinase C. Specifically, phosphate at position 660 increases protein kinase C's affinity for Ca2+ by almost
an order of magnitude compared with Ala at that position and about
2-fold compared with Glu. In addition, phosphate at this position
increases protein kinase C's low affinity interaction with
phosphatidylserine by an order of magnitude. These data suggest that
phosphate on Ser-660 may form stabilizing contacts with determinants in
the C2 domain of protein kinase C (see Fig. 10). This domain contains
the Ca2+ binding site, an aspartate-lined mouth formed by
two ends of a strand-rich domain, as well as determinants for
binding acidic phospholipids (8). In contrast, there is no significant
difference in membrane affinity for the mutants and wild-type enzyme in
the presence of diacylglycerol. Diacylglycerol's binding site is in the C1 domain, a separate membrane-targeting domain that is not allosterically regulated by the Ca2+ site (34); presumably
when this domain drives the membrane interaction, the contribution of
Ser-660 in allowing the C2 domain to bind to membranes is too small to
be apparent or, alternatively, the binding of diacylglycerol may
compensate for conformational changes ordinarily mediated by the
negative charge at position 660.
One possible explanation for the effect of negative charge at Ser-660
on C2 domain interactions is that the phosphate (or Glu) at position
660 structures part of the Ca2+ binding site by providing
coordinating ligands for Ca2+. Another possibility is that
phosphorylation of Ser-660 induces a conformational change that
structures the Ca2+ binding site for higher affinity
binding of Ca2+. The possibility that the carboxyl terminus
regulates the Ca2+ binding site is supported by two
findings. First, protein kinase C I and
II, which differ only in
the carboxyl-terminal 50 residues, have different Ca2+
affinities.4 Second, a regulatory
domain-directed RNA inhibitor binds protein kinase C
I and
II
with different affinities ((35),4 suggesting this molecule
binds a surface containing determinants shared by the regulatory domain
and carboxyl terminus. The findings that low affinity membrane binding
and Ca2+ binding, both of which are mediated by the C2
domain, are of decreased affinity for the S660A mutant are consistent
with carboxyl-terminal interactions regulating the Ca2+
binding (C2) domain.
The phosphorylation motif of a Ser or Thr bracketed by two aromatic hydrophobic residues occurs at the carboxyl terminus of a number of kinases, presenting the possibility that phosphorylation here might provide a general mechanism for structuring protein kinases. For example, protein kinase B (Akt) is markedly stimulated by phosphorylation of the Ser in its hydrophobic motif (11). Unlike protein kinase C, this phosphorylation results from cell stimulation and is catalyzed by another kinase. Similarly, S6 kinase has a hydrophobic phosphorylation motif at the carboxyl terminus of its kinase core; rapamycin treatment of Swiss 3T3 cells promotes the dephosphorylation of the Ser at this position, an event that is accompanied by inactivation of the kinase (10). Thus, phosphorylation at this hydrophobic phosphorylation motif may provide one mechanism for anchoring the carboxyl termini of kinases in such a way as to lock the enzymes in a catalytically favorable conformation.
ConclusionIn summary, studies with protein kinase C II
reveal that the role of the hydrophobic phosphorylation motif
FXXF(S/T)(F/Y) is to stabilize both the kinase
core and regulatory moiety of protein kinase C. The possibility that
phosphate here tethers the carboxyl terminus away from the active site,
providing interactions with determinants in the regulatory moiety, is
supported by the finding that phosphate at Ser-660 increases the
enzyme's affinity for Ca2+ and promotes substrate
binding.
We thank Maritess Jamosmos for assistance in cell culture and protein kinase C expression.