Affinity Purification of a Human RNA Polymerase II Complex Using Monoclonal Antibodies against Transcription Factor IIF*

(Received for publication, February 14, 1997, and in revised form, February 24, 1997)

Helen Cho , Edio Maldonado and Danny Reinberg Dagger

From the Howard Hughes Medical Institute, Division of Nucleic Acid Enzymology, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
CONCLUSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES


ABSTRACT

Five different monoclonal antibodies that immunoreact with RAP74, the large subunit of general transcription factor (TF) IIF, were produced and characterized. Using one of these antibodies, an affinity purification procedure was devised to isolate a human RNA polymerase II complex. This procedure is fast, simple, and reproducible and does not require extensive purification. The RNA polymerase II complex isolated using this procedure contains SRB (suppressor of RNA polymerase B) polypeptides, transcription factors IIE and IIF, limiting amounts of TFIIH, and the TATA-binding protein, but was devoid of TFIIB.


INTRODUCTION

Accurate initiation of transcription by RNA polymerase II (RNAPII)1 is a complex process that requires six general transcription factors (GTF; known as TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH) in addition to RNAPII and regulatory factors (reviewed in Refs. 1 and 2). The formation of a transcription complex begins with the recognition of the TATA motif by the TATA-binding protein (TBP) subunit of TFIID. The resulting protein-DNA complex provides a recognition site for the other factors that can enter either sequentially or as components of a pre-assembled complex, called the "RNAPII holoenzyme" (reviewed in Refs. 2-4).

A combination of genetic and biochemical experiments with the yeast Saccharomyces cerevisiae uncovered the existence of an RNAPII complex and demonstrated its biological significance (5, 6). The level of conservation between the transcription systems of yeast and higher eukaryotes suggested that a similar RNAPII complex exists in mammals. The first indication that such a complex exists in higher eukaryotes came from studies using rat liver extracts (7). It was found that antibodies directed against the CDK7/MO15 subunit of TFIIH immunoprecipitate RNAPII and the entire set of GTFs, except TFIIA. The immunoprecipitates could support RNAPII transcription in vitro. This was taken as evidence for a large RNAPII holoenzyme in mammals; however, it was not determined whether the immunoprecipitated material represented a single "holoenzyme" complex or many TFIIH·GTF complexes, as previous studies have shown that TFIIH can independently interact with many of the GTFs (8); nor was it demonstrated that the complex contained mammalian homologues of yeast SRB (for suppressor of RNA polymerase B; Refs. 9-13) proteins. The SRB genes were identified as supressors of the cold-sensitive phenotype associated with the partial truncation of the carboxyl-terminal domain (CTD) of RNAPII in S. cerevisiae (9, 14, 15). The CTD is an unusual motif present at the carboxyl terminus of the largest subunit of RNAPII, composed of a heptapeptide repeated 26 times in yeast and 52 times in mammals. The SRB proteins are hallmarks of the yeast RNAPII holoenzyme and distinguish the mammalian complexes as true RNAPII complexes.

Confirmation of the existence of mammalian RNAPII complexes came from studies using calf thymus and HeLa cells. The calf thymus complex contains substoichiometric amount of the GTFs TFIIE and TFIIH and cannot respond to activators (12), while the HeLa cell complex contains stoichiometric amounts of TFIIE and TFIIF and a substoichiometric amount of TFIIH and can respond to activators (13). More significantly, both complexes contain homologues of yeast SRB proteins: hSRB7 (12, 13) and cyclin C/CDK8 (hSRB11/hSRB10; Ref. 13).

From the studies described thus far, it is clear that the RNAPII complexes isolated contain different subsets of the GTFs. Adding complexity to the RNAPII complexes are the findings demonstrating that the mammalian RNAPII complexes contain a large number of other polypeptides, some of which may play important roles in nucleotide excision repair, DNA double strand break repair and/or cell cycle check point control (13), and chromatin remodeling (16); however, the majority remain elusive.

A known polypeptide that exists in stoichiometric amount with respect to the largest subunit of RNAPII in both the yeast (6, 15) and the human RNAPII complex (13) is transcription factor IIF. The subunits of TFIIF, RAP30 and RAP74, were first identified through their ability to interact with immobilized RNAPII (17). Soon after, TFIIF was independently purified as an essential RNAPII initiation factor (18-20). In addition to its role in initiation, TFIIF performs additional functions that increase the specificity and efficiency of RNAPII transcription. By stably associating with RNAPII, TFIIF can increase the rate of transcription elongation (19, 21-25). Additionally, TFIIF prevents spurious initiation by inhibiting, and reversing, the binding of RNAPII to non-promoter sites on DNA, drawing comparisons with the bacterial sigma  factors (26, 27).

We have taken advantage of the physical association between TFIIF and the human RNAPII complex to immunoaffinity purify a human RNAPII complex using monoclonal antibodies (mAbs) that recognize the large subunit of TFIIF. We also report the characterization of five different anti-RAP74 mAbs.


MATERIALS AND METHODS

Buffers

Buffer C contained 20 mM Tris-HCl, pH 7.8, 0.2 mM EDTA, pH 8.0, 1 mM dithiothreitol, 20% (v/v) glycerol (otherwise indicated), and 1 mM phenylmethylsulfonyl fluoride. TTBS (× 10) solution contained 100 mM Tris-HCl, pH 7.5, 2 M NaCl, and 0.5% (v/v) Tween 20. Laemmli buffer (× 1) contained 2% (w/v) SDS, 100 mM dithiothreitol, 60 mM Tris-HCl, pH 6.8, 0.001% (w/v) bromphenol blue, and 10% (v/v) glycerol.

Proteins

Escherichia coli BL21 (DE3) transformants containing RAP74 deletion constructs (ZB317, ZB304, ZB325, ZB275, ZB329, and ZB370) were a kind gift from Dr. Z. Burton (28). HeLa cell nuclear extracts were fractionated on a phosphocellulose (Sigma) column as described previously (29). Proteins eluting in the 0.3-0.5 M KCl wash were dialyzed against buffer C containing 0.1 M KCl and loaded onto a DEAE-cellulose (Whatman, DE52) column as described previously for the purification of TFIIF (19). Bound proteins were eluted with buffer C containing 0.5 M KCl and dialyzed to 0.15 M KCl prior to immunoaffinity purification. Protein factors used in the in vitro transcription reactions were purified as follows. Recombinant human TBP (30), TFIIB (31), TFIIE-p56 (32), TFIIE-p34 (32), TFIIF-RAP30 (17), and TFIIF-RAP74 (28, 33, 34) were isolated from bacteria using previously published procedures (35). TFIIE and TFIIF activities were reconstituted by mixing the isolated recombinant subunits as described (35). Human RNAPII and TFIIH were isolated from HeLa cells. RNAPII was affinity-purified using monoclonal antibodies recognizing the CTD (36). This procedure yields a protein preparation that is greater than 99% pure as judged by silver staining (35). TFIIH (phenyl-Superose) was purified as described previously (37) or by affinity purification using monoclonal antibodies recognizing the 89-kDa subunit of TFIIH, ERCC3, using a procedure to be published elsewhere.2

Hybridomas and mAbs

Hybridomas producing monoclonal antibodies were generated against Ni-NTA-agarose-purified recombinant RAP74 polypeptides according to standard procedures (38). Positive clones were selected by enzyme-linked immunosorbent assay as well as by Western blot analysis. The supernatants were analyzed for their capability to recognize RAP74 in Western blots (data not shown). The isotype of each monoclonal antibody was determined using the ISOStrip mouse monoclonal antibody isotyping kit from Boehringer Mannheim.

Western Blot Analysis

For epitope mapping, extracts from E. coli BL21 (DE3) strains expressing RAP74 deletion proteins (28) were prepared and separated by electrophoresis on a 15% SDS-polyacrylamide gel. Proteins were transferred to nitrocellulose membranes, which were subsequently blocked for 30 min in 3% nonfat dry milk in 1 × TTBS, then washed extensively with 1 × TTBS and incubated with ascitic fluid derived from each of the anti-RAP74 mAbs at a dilution of 1:500 in 0.1% bovine serum albumin and 1 × TTBS. To prevent the mAbs from nonspecifically cross-immunoreacting with bacterial proteins, E. coli BL21 (DE3) extracts (50 µg/ml) prepared from a strain lacking RAP74 were mixed with the mAbs during the incubation. Alkaline phosphatase-conjugated anti-mouse IgG antibodies (Promega) were used as secondary antibodies. After incubation with the secondary antibodies, the membranes were washed with 1 × TTBS and developed with nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (Bio-Rad) according to the manufacturer's instructions.

For the characterization of the anti-RAP74 affinity-purified RNAPII complex, an aliquot of each fraction (30 µl) was loaded onto a 5-15% SDS-polyacrylamide gradient gel. Polypeptides were transferred to polyvinylidene difluoride membrane (Millipore) and probed with various antibodies as indicated. Western analysis was performed as above without the addition of E. coli extract.

Immunoprecipitations

Ascitic fluid, containing anti-RAP74 mAbs (1C11/G7, 4F8/G11, 6H10/F10, 7B3/E10, and 7E7/G11, 20 µl) were each bound to 10 µl of protein G-agarose beads (Boehringer Mannheim) for 1.5 h at 20 °C. The beads were subsequently washed with 1 ml of phosphate-buffered saline, followed by 1 ml of buffer C containing 0.1 M KCl, 0.1% (v/v) Nonidet P-40, and 0.05% (v/v) Triton X-100. The beads were then incubated for 2 h at 4 °C with the DEAE-cellulose column-bound fraction (150 µg, see "Proteins") that had been dialyzed in buffer C containing 0.1 M KCl, 0.1% Nonidet P-40 (v/v), and 0.05% Triton X-100 (v/v). Each immune complex was washed three times with 1 ml of buffer C containing 0.15 M KCl, 0.1% Nonidet P-40 (v/v), and 0.05% Triton X-100 (v/v), and 1 ml of buffer C containing 0.05 M KCl. The immune complexes were eluted by boiling in 2 × Laemmli buffer and analyzed by Western blot using either affinity-purified rabbit polyclonal antibodies against the CTD of RNAPII or RAP74. For peptide inhibition experiments, 5 µg of peptide were preincubated with mAbs bound to protein G beads in of buffer C containing 0.1 M KCl for 30 min at 20 °C, then washed extensively with buffer C containing 1 M KCl and buffer C containing 0.05 M KCl prior to incubation with the DEAE-cellulose protein fraction. Immune complexes were analyzed by Western blot for RAP74. The following peptides containing sequences of RAP74 were synthesized by HHMI/UCSF Protein Structure Laboratory (a, 231IPKAKKKAPLAKGGRKKKKKKGSDDEAF258; b, 202ASELRIHDLEDDLEMSSDASDASGE226; c, 411GKRVSEMPAAKRLRLDTGPQSLSGK435; d, 436STPQPPSGKTTPNSGDVQVTEDAVR460).

Electrophoretic Mobility Shift Assay

The TAB (promoter DNA complex containing TBP, TFIIA, and TFIIB) and TBPolFEH (promoter DNA complex containing TBP, TFIIB, RNAPII, TFIIF, TFIIE, and TFIIH) complexes were formed on a 32P-labeled DNA fragment containing sequences of the adenovirus major late promoter (Ad-MLP) as described previously (39). Protein G-purified anti-RAP74 and anti-FLAG (Eastman Kodak Co.) mAbs were added (8 ng/µl) to the TAB and TBPolFEH complexes and incubated for an additional 30 min at 28 °C. The complexes were separated by electrophoresis on a 3.5% native polyacrylamide gel and visualized by autoradiography.

Anti-RAP74 Immunoaffinity Purification of RNAPII Complex

Monoclonal antibody 7B3/E10 or anti-beta -galactosidase (Promega) was covalently cross-linked to protein G-agarose beads (1 ml) using 20 mM dimethylpimelidate as described (40). Prior to incubation with the protein sample, the resin was equilibrated with buffer C containing 0.15 M KCl, 0.1% Nonidet P-40 (v/v), and 0.05% (v/v) Triton X-100. Proteins derived from the DEAE-cellulose column 0.5 M wash were dialyzed against buffer C containing 0.15 M KCl, 5 mM MgCl2, 0.1% (v/v) Nonidet P-40, and 0.05% (v/v) Triton X-100. An aliquot of the dialyzed fraction (5 mg) was subsequently incubated with 0.1 volume of the protein fraction with protein G-agarose beads for 30 min at 4 °C, which was then removed by centrifugation at 500 × g for 5 min. This precleared fraction was incubated with the resin (1 ml) for 3 h at 4 °C. The resin was washed with 30 column volumes of buffer C containing 0.2 M KCl, followed by 10 column volumes of buffer C containing 0.05 M KCl (Fig. 4A, (W)). The resin was then incubated (for 15 min at 4 °C) with 1 column volume of buffer C containing 0.05 M KCl and control peptide a (2.5 mg/ml; Fig. 4A, (C)). The RNAPII complex was then eluted by incubating the resin with 1 column volume of peptide c containing the epitope sequences (2.5 mg/ml in buffer C containing 0.05 M KCl) for 15 min at 28 °C or for 1 h at 4 °C (fraction 1). Three further fractions were collected by eluting each with 1 column volume of buffer C containing 0.05 M KCl (fractions 2-4). For transcription analysis, fractions 1 and 2 (2 ml) were pooled and concentrated to 0.5 ml on a concentrator (Millipore) and dialyzed against buffer C containing 0.1 M KCl. The gel filtration analysis was performed with fractions 1 and 2 (2 ml), which were concentrated (to 1 ml) and dialyzed against buffer C containing 0.5 M KCl, 50% (v/v) glycerol, 0.01% (v/v) Nonidet P-40, and 0.05% (v/v) Triton X-100. Anti-RAP74 affinity-purified complexes (100 µl) were loaded onto a 4-ml (0.5 cm × 20 cm) Sepharose CL-4B (Pharmacia) column. Fractions of 120 µl were collected at a flow rate of 30 µl/min in buffer C containing 0.5 M KCl, 20% (v/v) glycerol, 0.01% (v/v) Nonidet P-40, and 0.05% (v/v) Triton X-100. For Western blot analysis, every other fraction was precipitated with trichloroacetic acid, washed with 80% ethanol, and loaded onto a 5-15% SDS-polyacrylamide gradient gel. The gel filtration step was performed also with a cruder fraction derived from the DEAE-cellulose column (the input used for the affinity chromatography). The protein pool (144 mg/170 ml) was concentrated on a 40-ml S-Sepharose ion exchange column (98 mg/70 ml) before chromatography on the Sepharose CL-4B gel filtration column. The concentrated sample was dialyzed against buffer C containing 0.5 M KCl, 50% (v/v) glycerol, 0.01% (v/v) Nonidet P-40, and 0.05% (v/v) Triton X-100. Proteins (10.5 mg) were loaded onto the Sepharose CL-4B column (350 ml, 2.5 cm × 75 cm), and the column was developed with buffer C containing 0.5 M KCl, 20% (v/v) glycerol, 0.01% (v/v) Nonidet P-40, and 0.05% (v/v) Triton X-100. Fractions (1.2 ml) were collected at 2 ml/min. Every other third fraction was analyzed by Western blot analysis (20 µl) and for transcription (10 µl) in a reconstituted system containing only TBP, TFIIB, and TFIIH. Both Sepharose CL-4B columns were calibrated with thyroglobulin and blue dextran (Sigma).


Fig. 4. Immunoaffinity purification of the RNAPII complex. A, schematic diagram of the steps used during the anti-RAP74 affinity purification of the RNAPII complex. For details, see "Materials and Methods." B, Western blot analysis of the fractions of the immunoaffinity purification of the RNAPII complex. Each blot was probed for different polypeptides as indicated on the right of the panels. Lane 1, 1% of input (IN); lanes 2 and 8, wash fraction with buffer C containing 0.05 M KCl (W); lanes 3 and 9, fractions from the control peptide elution (C); lanes 4 and 10, fractions from peptide c elution; lanes 5-7 and 11-13, additional buffer elutions. C, silver stain of the anti-RAP74 affinity-purified RNAPII complex. M, molecular weight marker; lane 1, eluate of anti-beta -galalactosidase column (20 µl); lane 2, eluate from the anti-RAP74 column (20 µl); lane 3, core RNAPII. Subunits of core RNAPII (IIo, IIa, IIc, IId, IIe, IIf, IIg, IIh, IIi, and IIj) and TFIIF (RAP74 and RAP30) are indicated on the right side of the panel.
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Reconstituted Transcription Assays

Transcription reactions (40 µl) were reconstituted on a linear DNA containing the Ad-MLP fused to a 392-nucleotide G-less cassette (41). Transcription factors were purified as described above and were added as indicated. Factors used were human TBP (5 ng), TFIIB (5 ng), TFIIE (15 ng), TFIIF (23 ng), TFIIH (500 ng), and RNAPII (50 ng). alpha -Amanitin was added to the reaction mixture to 2 µg/ml prior to the addition of nucleotides as indicated. Products of the reactions were separated on a 7 M urea, 6% polyacrylamide gel and quantitated using a phosphorimager (Bio-Rad).


RESULTS AND DISCUSSION

Epitope Mapping of Anti-RAP74 Monoclonal Antibodies

A series of mAbs directed against the large subunit of TFIIF (RAP74) were generated. Immunization was performed with bacterially expressed, hexahistidine-tagged RAP74 that was purified by metal affinity chromatography. The particular epitopes recognized by the various antibodies were defined by two methods: immunoreactivity of RAP74 truncated polypeptides and immunoprecipitation studies.

Five different RAP74 mAbs (1C11/G7, 4F8/G11, 6H10/F10, 7B3/E10, and 7E7/G11) were selected and analyzed for their ability to recognize different truncated RAP74 polypeptides using Western blots (Fig. 1). This analysis allows us to approximate the domain of RAP74 recognized by various mAbs. For example, mAb 1C11/G7 (1C11) specifically recognizes polypeptide ZB275 (amino acids 407-517), while it does not recognize polypeptide ZB370 (amino acids 363-452). Therefore, the epitope recognized by mAb 1C11 resides in the boundary of amino acids 453-517. The same approach was applied to the other mAbs. Monoclonal antibodies 4F8/G11 (4F8) and 6H10/F10 (6H10) recognize an epitope located between amino acids 207 and 258, whereas mAbs 7B3/E10 (7B3) and 7E7/G11 (7E7) recognize an epitope within residues 407-452 in RAP74.


Fig. 1. Epitope mapping of RAP74 mAbs using deletion mutants. Top, protein extracts from E. coli BL21 (DE3) strains overproducing different RAP74 proteins, as indicated at the bottom of the panel, were resolved on a 15% SDS-PAGE for Western blot analysis. Different blots, containing the same polypeptides, were probed with the mAb depicted below the blots. Bottom, schematic representation of the RAP74 deletion proteins used in the upper panel and the epitopes that are recognized by each mAb as defined by Western blot analysis.
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We next analyzed whether the different RAP74 mAbs could immunoprecipitate TFIIF and TFIIF-associated factors such as RNAPII. Western blot analysis using polyclonal antibodies against the large subunit of RNAPII and RAP74 showed that four of the antibodies (4F8, 6H10, 7B3, and 7E7) did co-immunoprecipitate RNAPII (Fig. 2A). Monoclonal antibody 1C11 failed to immunoprecipitate TFIIF. Co-immunoprecipitation of RNAPII was not a result of cross-reactivity with RNAPII, since none of the antibodies directly immunoprecipitate RNAPII when a TFIIF-free RNAPII fraction was used (data not shown).


Fig. 2. Characterization of the specificity of the different mAbs by immunoprecipitations and electrophoretic mobility shift assays. A, monoclonal antibodies co-immunoprecipitate RNAPII. Different monoclonal antibodies were used to immunoprecipitate TFIIF and analyzed for their ability to co-immunoprecipitate RNAPII. The protein fraction used was the DEAE-cellulose, which contained TFIIF and RNAPII. Immunoprecipitation and Western blots were performed as described under "Materials and Methods." The antibodies used are indicated on the top of the lanes. IIa denotes the largest subunit of RNAPII, which was detected using polyclonal antibodies against the CTD. The lower panel indicates the immunoprecipitation of RAP74, which was detected using polyclonal antibodies. B, epitope mapping of the RAP74 mAbs using synthetic peptides to inhibit immunoprecipitation of RAP74. Synthetic peptides were preincubated with each mAb prior to incubation with the DEAE-cellulose protein fractions. Immunoprecipitations followed by Western blot analysis were performed as described under "Materials and Methods." Lanes 2, 5, 8, 12, and 16 show immune complexes formed without peptide preincubation. Lanes 3, 6, 11, 15, and 19 show immune complexes formed after preincubation with peptide a; lanes 4 and 7 show immune complexes after preincubation with peptide b; lanes 9, 13, and 17 show immune complexes after preincubation with peptide c; lanes 10, 14, and 18 show immune complexes formed after preincubation with peptide d. The mAbs used in the immunoprecipitation are indicated above the Western blot. Lane 1 is 10% of the input sample that was directly loaded onto the gel. C, monoclonal antibodies 6H10 and 7B3 change the electrophoretic mobility of the TBPolFEH preinitiation complex, but not of the TAB preinitiation complex intermediate. The TAB and TBPolFEH complexes were formed on the Ad-MLP as described under "Materials and Methods." The complexes were then incubated with protein G-purified mAbs for 30 min at 28 °C. Complexes were resolved on a native polyacrylamide gel as described under "Materials and Methods." Lane 1, the complexes in the absence of antibodies. All other lanes contain mAbs as indicated at the top of the panel.
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The epitopes recognized by the different antibodies were further defined by designing peptides overlapping the residues mapped above. The peptides were analyzed for their ability to specifically compete with the immunoprecipitation of RAP74. The regions of RAP74 that were recognized by the different antibodies, defined in Fig. 1, were analyzed by the ANTIGENIC program of the Genetics Computer Group (GCG Package). This algorithm generates a prediction of the antigenicity of a particular peptide sequence. Two overlapping peptides, each containing predicted antigenic sequences, were designed and synthesized for regions of RAP74 mapped by Western blot. Monoclonal antibody 1C11 failed to immunoprecipitate RAP74, and therefore was not used in this analysis. Peptides a and b were derived from amino acids 207-258 (recognized by mAbs 4F8 and 6H10), and peptides c and d were from amino acids 407-452 (recognized by mAbs 7B3 and 7B7).

The reactivities of mAbs 4F8 and 6H10, which recognize the same set of RAP74 deletion mutants (Fig. 1), were distinguished by this method. Immunoprecipitation of RAP74 by antibody 4F8 was competed by peptide a, but not peptide b (Fig. 2B, lanes 3 and 4), whereas immunoprecipitation by antibody 6H10 was competed by peptide b, but not peptide a (Fig. 2B, lanes 6 and 7). This demonstrates that mAbs 4F8 and 6H10 recognize unique epitopes located between amino acids 231-258 (peptide a) and 202-226 (peptide b), respectively. In a similar manner, mAbs 7B3 and 7E7 were further characterized. Immunoprecipitations of RAP74 by mAbs 7B3 and 7E7 were specifically competed by peptide c (Fig. 2B, lanes 9 and 13), but not by peptides d or a (Fig. 2B, lanes 10, 11, 14, and 15). This inhibition by peptide c was specific to mAbs 7B3 and 7E7, because this peptide has no effect on mAb 4F8 (Fig. 2B, lane 17). Therefore, we concluded that the epitope(s) recognized by mAbs 7B3 and 7E7 is (are) located between amino acids 411 and 435 (peptide c).

Monoclonal Antibodies Recognize TFIIF within a Transcription Preinitiation Complex

Our immediate goal was to isolate antibodies that allow affinity purification of TFIIF-containing protein complexes, i.e.. RNAPII complexes. We reasoned that mAbs recognizing RAP74 within a transcription pre-initiation complex, could, in principle, recognize RAP74 present in RNAPII complexes. Toward this goal, we analyzed whether the different mAbs affected the migration of the TBPolFEH complex formed on the Ad-MLP (Fig. 2C, lane 1; top). As a control, the preinitiation complex intermediate TAB (Fig. 2C, lane 1; bottom), which lacks TFIIF but contains TBP, TFIIA, and TFIIB, was also formed on the Ad-MLP.

Monoclonal antibodies 6H10 and 7B3 were found capable of supershifting the complex containing TFIIF, but not the TAB complex (Fig. 2C, lanes 5 and 6). In contrast mAbs 1C11, 4F8, and 7E7 have no effect on the complexes (Fig. 2C, lanes 2, 4, and 7). These observations allowed us to distinguish mAbs 7B3 and 7E7. The epitope recognized by mAbs 7B3 and 7E7 is contained between amino acids 411 and 435 in RAP74 (Fig. 2B), yet mAb 7B3, but not mAb 7E7, supershifted the TBPolFEH complex (Fig. 2C, lanes 6 and 7). None of the mAbs affected transcription, under conditions where rabbit polyclonal-anti-RAP74 antibodies inhibited transcription (data not shown).

From the studies described above, we concluded that mAbs 7B3 and 6H10 are likely to be effective in isolating TFIIF-containing RNAPII complexes, as both antibodies co-immunoprecipitated RNAPII and were capable of recognizing RAP74 epitopes within the transcription pre-initiation complex. All of the mAbs were classified as IgG1 and kappa  isotype. The properties of the different monoclonal antibodies are summarized in Fig. 3.


Fig. 3. Summary of the characteristics of the anti-RAP74 mAbs. Figure is a schematic representation of human RAP74. The domains that interact with the small subunit of TFIIF (RAP30) and RNAPII (28) and the epitopes recognized by each mAb characterized in this analysis are depicted. The other properties of the mAbs are also indicated.
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Immunoaffinity Purification of RNA Polymerase II Complex

Our previous studies have demonstrated that the conventionally purified human RNAPII complex contains stoichiometric amounts of TFIIF, with respect to the largest subunit of RNAPII (13). Therefore, the mAb 7B3 was used to affinity purify an RNAPII complex from a HeLa cell-derived crude protein fraction.

Anti-TFIIF and anti-beta -galactosidase mAbs were covalently linked to agarose beads coated with protein G and used in the purification as described in Fig. 4A and "Materials and Methods." Protein fractions from the second step of the conventional chromatographic RNAPII complex purification procedure (13) was used as input material for the affinity purification. Briefly, HeLa cell nuclear extracts were fractionated on a phosphocellulose column as described previously (29). The 0.3-0.5 M KCl wash was applied onto a DEAE-cellulose column, and the bound proteins were eluted as described previously (19). This fraction was dialyzed to 0.15 M KCl before loaded onto the antibody affinity column (Fig. 4A; DEAE 52 0.5 M). Proteins that bound nonspecifically to the column were washed with buffer C containing 0.2 M KCl, 0.1% (v/v) Nonidet P-40, and 0.05% (v/v) Triton X-100, followed by a second wash with the same buffer containing 0.05 M KCl (Fig. 4A, (W)). Bound proteins were further washed with a buffer containing 0.05 M KCl and the irrelevant peptide a (see "Materials and Methods"; Fig. 4A, (C)). Proteins that remain bound were eluted from the column with peptide c, which contains the epitope recognized by the 7B3 mAbs.

Fractions from the control and 7B3 columns were analyzed for the presence of different polypeptides using Western blot analysis and silver staining. The results from the Western blot analysis demonstrate that the column containing the mAb 7B3, but not the control column containing the beta -galactosidase mAb, retained the large subunit of TFIIF (RAP74), the largest subunit of RNAPII (IIa), and CDK8, the human homologue of SRB10 (10, 11), and a hallmark of the yeast RNAPII holoenzyme (9) among other factors (Fig. 4B and data not shown; see below). These polypeptides were specifically retained by the mAb 7B3 column, and specifically eluted with peptide c (Fig. 4B, lanes 4-7), since these polypeptides were absent in the eluate obtained with the unrelated peptide a (Fig. 4B, lane 3). Moreover, silver staining of an SDS-polyacrylamide gel, containing the protein pool derived from the control and 7B3 mAb columns, reveals that a large number of polypeptides are present in the peptide-eluate fraction derived from the 7B3 mAb column (Fig. 4C, lane 2). No polypeptides were detected in the peptide-eluate fraction derived from the control column (Fig. 4C, lane 1).

Analysis of the polypeptides present in the silver-stained gel reveals the presence of the different subunits of RNAPII (Fig. 4C, compare lanes 2 and 3). The two most predominant polypeptides, however, correspond to the two subunits of TFIIF. This is not an unexpected result, as the fraction used in the affinity purification was crude and contains "free" TFIIF as well as TFIIF within the RNAPII complex. We have observed that fractionation of the input sample (DEAE-cellulose) on a DEAE-5PW column prior to affinity chromatography results in the separation of free TFIIF from the RNAPII complex (data not shown). Consistent with our previous report (13), a large number of unidentified polypeptides were also present in the affinity-purified sample.

To analyze whether the affinity-purified sample represents one complex or interactions of TFIIF with multiple complexes, the sample was analyzed by gel filtration on a Sepharose CL-4B column. Sepharose CL-4B resolves polypeptides/complexes between 6 × 104 and 2 × 107 daltons and was previously used successfully to characterize the RNAPII complex (13). Fractionation of the affinity-purified sample on this column resulted in the resolution of two peaks containing RNAPII, as detected by Western blot using antibodies against the largest subunit of RNAPII (Fig. 5A). The smaller peak (fraction 21-25) eluted with an apparent mass of approximately 1.3 to 1.5 MDa, whereas the second peak (fraction 15-17) of RNAPII eluted with an apparent mass larger than 2 MDa (blue dextran). When the input sample used in the affinity purification step was directly loaded onto a similar gel filtration column, two distinct RNAPII complexes were also resolved (Fig. 5B). The resolution of two RNAPII-containing peaks is not unexpected. The human RNAPII previously isolated, using multiple chromatographic steps, was estimated to have a mass of approximately 1.5 MDa, and found to sediment close to the 60 S ribosomal subunit (13). However, the RNAPII in HeLa cell nuclear extracts sedimented on sucrose gradients in a broad peak, between the 60 S and 80 S ribosome subunits (13). These previous results were interpreted to suggest that factors present in a large RNAPII complex were removed during extensive chromatography. Since the purification procedure described in the present studies involves only three steps, it is possible that the integrity of a larger RNAPII-containing complex is maintained. We suspect that the smaller complex isolated in the present studies corresponds to the RNAPII complex isolated previously using conventional chromatography. In agreement with this assessment, we found that the smaller RNAPII complex copurifies with TFIIF, TFIIE, and CDK8 (human SRB10; Refs. 9-11), the catalytic subunit of the DNA-dependent protein kinase (DNAPKcs; Ref. 42), and other previously identified factors such as cyclin C (human SRB11; Refs. 9-11) and human SRB7 (Fig. 5 and data not shown). This complex was transcriptionally active in an assay reconstituted only with TBP, TFIIB, and TFIIH (Fig. 5B and see below). The larger RNAPII-containing complex copurifies with DNAPKcs, but surprisingly, this complex was devoid of the GTFs, as exemplified by the absence of TFIIF (Fig. 5 and data not shown). Consistently, this complex was capable of directing transcription in an assay reconstituted with all the GTFs (data not shown), but was not capable of directing transcription in a reconstituted assay lacking TFIIE and/or TFIIF (Fig. 5B). Since the complexes resolved on the Sepharose CL-4B column were isolated by affinity chromatography using monoclonal antibodies against the large subunit of TFIIF, and only the smaller complex was immunoreactive to subunits of TFIIF, we conclude that the two complexes resolved on the gel filtration column do not represent different entities existing in vivo, and suggest that these complexes were generated during chromatography from a larger RNAPII-containing complex.


Fig. 5. Analysis of affinity and conventionally partially purified RNAPII complexes by gel filtration chromatography. A, the affinity-purified complex was applied onto a Sepharose CL-4B column as described under "Materials and Methods." Odd-numbered fractions (11-29) were analyzed for the presence of the large subunit of RNAPII (RPB1), DNAPKcs, CDK8, TFIIE-p34, and TFIIF-RAP74 by Western blot as indicated. B, the partially purified RNAPII complex used in the affinity purification step (DEAE-cellulose-bound fraction) was directly applied onto a Sepharose CL-4B gel filtration column as described under "Materials and Methods." Every third fraction (75-135) was analyzed for the presence of the large subunit of RNAPII (RPB1), TFIIF-RAP74, hSRB7 (B) by Western blot analysis and transcription activity. The input sample revealed two immunoreactive polypeptides to the human SRB7 antibodies. The faster migrating form of SRB7 cofractionated with the transcriptionally active complex, whereas the transcriptionally inactive, larger complex contained exclusively the slower migrating form of SRB7. The implication of this observation, if any, remains to be determined. Transcription activity was reconstituted with the column fractions supplemented with the general transcription factors TBP, TFIIB, and TFIIH. The autoradiograph of the 392-nucleotide transcript is shown (bottom panel). Fraction numbers are indicated on top of each panel. Five percent of input material of each column was loaded in lanes labeled In.
[View Larger Version of this Image (43K GIF file)]


Functional Characterization of the Affinity-purified RNAPII Complex

Our previously characterized RNAPII complex, purified by conventional chromatography, was found to contain stoichiometric amounts of TFIIE, TFIIF, and limiting amounts of TFIIH, and was devoid of TFIIB, TBP, and TBP-associated factors (13). We next functionally analyzed which GTFs were present in the complex purified by affinity chromatography. Transcription reactions were reconstituted with the GTFs and either core RNAPII that was affinity-purified over an anti-CTD mAb column (Fig. 4C, lane 3), or the RNAPII complex purified on an anti-RAP74 (mAb 7B3) column (Fig. 4C, lane 2). The results presented in Fig. 6 demonstrate that the affinity-purified complex contains a transcriptionally active form of RNAPII (lane 10). Transcription was sensitive to low concentrations of alpha -amanitin (Fig. 6A, lane 14). Transcription directed by the core RNAPII was, as expected, dependent on each of the GTFs (Fig. 6A, lanes 2-7); however, transcription directed by the RNAPII complex was only dependent upon TFIIB (Fig. 6A, lane 9). The omission of TFIIE or TFIIF from the reconstituted system was without an effect on transcription directed by the RNAPII complex (Fig. 6A, lanes 11 and 12). Consistent with our previous findings, we observed that the RNAPII complex contains limiting amounts of TFIIH (Fig. 6A, lane 13); however, contrary to our previous observations, we also found limiting amounts of TBP (Fig. 6A, lane 8). The presence of TBP within the affinity-purified RNAPII complex, but not in the conventionally purified RNAPII complex (13), is perhaps not surprising since our previously characterized RNAPII complex included a larger number of chromatographic steps that, most likely, removed TBP from the complex. The important finding, however, is that both conventionally and affinity-purified RNAPII complexes contain TFIIE and TFIIF, are devoid of TFIIB, and contain limiting amounts of TFIIH.


Fig. 6. Transcription of the affinity-purified RNAPII complex depends upon the addition of TBP, TFIIB, and TFIIH. A, lanes 1-7 show transcription reconstituted with core RNAPII. Lanes 8-14 show transcription reconstituted with the RNAPII complex purified by immunoaffinity chromatography using mAb 7B3. Lane 1, complete reaction; lanes 2-13, the indicated factor was omitted from the reconstituted transcription assay. Lane 14 is identical to lane 10, but alpha -amanitin was added. B, comparison of the transcriptional activity of core RNAPII and immunoaffinity-purified RNAPII complex. Equivalent amounts (normalized by Western blot analysis using polyclonal antibodies against RPB1, RPB2, and RPB7) of the RNAPII preparations were added to reconstituted transcription assays containing the entire set of GTFs at a saturating level. Lane 1 shows that the reconstituted transcription assay is dependent on the addition of RNAPII. The relative transcriptional activity of each lane, quantitated by phosphorimager, is shown as a bar graph above the autoradiogram. C, comparison of the transcriptional activity of core RNAPII (lanes 1-4) and immunoaffinity-purified RNAPII complex (lanes 5-7) using the subset of factors (TBP, TFIIB, and TFIIH, TBP/IIB/IIH) that are absent or limiting in the affinity-purified RNAPII complex. Additions were as indicated on the panel.
[View Larger Version of this Image (31K GIF file)]


To further compare the transcription activity directed by core RNAPII and the RNAPII complex, the exact amount of RNAPII in each preparation was estimated using quantitative Western blots and antibodies against three subunits of RNAPII: RPB1, RPB2, and RPB7 (data not shown). Similar amounts of core RNAPII and affinity-purified RNAPII complex were added to reconstituted transcription systems containing all of the GTFs (Fig. 6B), or only TBP, TFIIB, and TFIIH (Fig. 6C, lanes 2-7). We found that the core RNAPII was approximately 1.5-fold more active than the RNAPII complex in a reconstituted system containing all the GTFs at saturating levels (Fig. 6B). In agreement with the results presented above (Fig. 6A), we found that only the affinity-purified RNAPII complex could direct transcription in a reconstituted system lacking TFIIE and TFIIF (Fig. 6C). The addition of TFIIE and/or TFIIF did not increase the levels of transcription observed with the affinity-purified RNAPII complex (data not shown). Therefore, the results presented above, collectively, allow us to conclude that, regardless of the method used to purify the RNAPII complex, transcription factors IIE and IIF are present at saturating levels and transcription factor IIB is absent from the human RNAPII complex. Limiting amounts of TFIIH are consistently observed in the human RNAPII complex. The presence of limiting amounts of TFIIH in the RNAPII complex, and of TBP in the affinity-purified RNAPII complex, is most likely because TFIIH and TBP can interact with many components of the complex. This suggests that TFIIH and TBP are not integral components of the human RNAPII complex.


CONCLUSION

In these studies we described an affinity purification procedure, using monoclonal antibodies against the large subunit of TFIIF, that permits the isolation of the human RNAPII complex. The procedure was based on previous observations, demonstrating that the yeast (5, 6) and human (13) RNAPII complexes contain stoichiometric amounts of TFIIF. The procedure is fast, simple, and reproducible and yields a transcriptionally active form of the human RNAPII complex. In addition, the present studies also describe five different monoclonal antibodies, which recognize different regions of the large subunit of TFIIF. These reagents will be valuable tools in attempts to understand the roles of TFIIF during the transcription cycle.

The purification procedure described here for the human RNAPII complex resulted in the isolation of a multiprotein complex. Transcription assays and Western blot analyses indicate that the complex contains stoichiometric amounts of TFIIE and TFIIF and limiting amounts of TFIIH, and is devoid of TFIIB. Identical results were obtained with the human RNAPII complex isolated using an elaborate conventional chromatographic procedure (13).

Different RNAPII complexes have been isolated (6, 12, 13, 15). Each of these complexes contains a different subset of the GTFs. For example, the yeast complex isolated by Kornberg and colleagues contains only TFIIF (6), which is present in stoichiometric amounts, whereas the yeast complex isolated by Young and colleagues contains stoichiometric amounts of TFIIB, TFIIF, and TFIIH but is devoid of TFIIE and TBP (5, 15). As for the mammalian complexes, the complex isolated from calf thymus contains substoichiometric amounts of TFIIE and TFIIH and is devoid of TBP, TFIIB, and TFIIF (12), while the conventionally purified human RNAPII complex contains TFIIE and TFIIF in stoichiometric amounts, contains limiting amounts of TFIIH, and is devoid of TFIIB and TBP (13). Similar results were obtained with the affinity-purified human RNAPII complex. The only difference was the presence of limiting amounts of TBP in the affinity-purified complex. We suspect that TBP, TFIIB, and TFIIH are not integral components of the RNAPII complex, and their association may simply reflect the ability of these factors to interact with multiple components in the RNAPII complexes. Interestingly, each of the factors we found absent in the RNAPII complex have been suggested to participate in steps of the transcription cycle that are subject to regulation by activators (43).

The "RNA polymerase holoenzyme" term was initially applied to the bacterial RNA polymerase, which contains the core polymerase and sigma  subunits (44). An important property of the bacterial RNA polymerase holoenzyme is its ability to recognize promoters and initiate transcription. In higher eukaryotic systems, such a complex has yet to be defined. As discussed above, none of the RNA polymerase II complexes thus far isolated do have a complete set of GTFs. These complexes are not, therefore, capable of specifically initiating transcription. Additional factors (GTFs) are required. We therefore believe that using the term holoenzyme, when referring to the eukaryotic RNAPII complexes isolated thus far, is misleading. We suggest that they be termed RNAPII complexes. We do not imply, however, that there are no RNA polymerase II holoenzymes complexes in vivo. A purification scheme with minimal manipulations is more likely to result in the purification of intact and physiological RNA polymerase II complex. In this respect, the procedure described here should provide the basis to isolate true RNA polymerase II holoenzyme complexes from eukaryotic cells.


FOOTNOTES

*   This work was supported by Grant GM37120 from the National Institutes of Health and from the Howard Hughes Medical Institute (both to D. R.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Dagger    To whom correspondence should be addressed. Tel.: 908-235-4195; Fax: 908-235-5294; E-mail: reinbedf{at}umdnj.edu.
1   The abbreviations used are: RNAPII, RNA polymerase II; GTF, general transcription factor; TF, transcription factor; TBP, TATA box-binding protein; SRB, suppressor of RNA polymerase B; CTD, carboxyl-terminal domain; mAb, monoclonal antibody; Ad-MLP, adenovirus major late promoter; TTBS, Tris-buffered saline solution + Tween 20; TAB complex, promoter DNA complex containing TBP, TFIIA, and TFIIB; TBPolFEH complex, promoter DNA complex containing TBP, TFIIB, RNAPII, TFIIF, TFIIE, and TFIIH; DNAPKcs, catalytic subunit of the DNA-dependent protein kinase.
2   G. Le Roy, L. Weis, R. Drapkin, and D. Reinberg, submitted for publication.

ACKNOWLEDGEMENTS

We thank L. Weis and T. Kim for the gift of recombinant RAP74 polypeptide, Dr. R. Drapkin for the initial screening of the monoclonal antibodies, and Xiaoqing Sun for reagents and experiments. We also thank Dr. Z. Burton for the RAP74 deletion constructs and L. Weis and Dr. G. Orphanides for critical reading of the manuscript. We also acknowledge Dr. A. Jamett and Dr. P. Valenzuela from Bios-Chile I.G.S.A. and its subsidiary Austral Biologicals (San Ramón, CA) for producing the monoclonal antibodies and providing the ascitic fluids. We are grateful to Dr. E. Golemis from the Fox Chase Cancer Center for providing antibodies against the human RNAPII-RPB7 subunit, and to Dr. E. Nigg for anti-CDK8 serum.


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