Side Reactions Catalyzed by Ribulose-bisphosphate Carboxylase in the Presence and Absence of Small Subunits*

(Received for publication, September 4, 1996, and in revised form, December 12, 1996)

Matthew K. Morell Dagger , Jean-Marc Wilkin §, Heather J. Kane and T. John Andrews

From the Molecular Plant Physiology Group, Research School of Biological Sciences, Australian National University, P.O. Box 475, Canberra, Australian Capital Territory 2601, Australia

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
Acknowledgments
REFERENCES


ABSTRACT

The large subunit core of ribulose-bisphosphate carboxylase from Synechococcus PCC 6301 expressed in Escherichia coli in the absence of its small subunits retains a trace of carboxylase activity (about 1% of the kcat of the holoenzyme) (Andrews, T. J (1988) J. Biol. Chem. 263, 12213-12219). During steady-state catalysis at substrate saturation, this residual activity diverted approximately 10% of the reaction flux to 1-deoxy-D-glycero-2,3-pentodiulose-5-phosphate as a result of beta  elimination of inorganic phosphate from the first reaction intermediate, the 2,3-enediol form of ribulose bisphosphate. This indicates that the active site's ability to stabilize and/or retain this intermediate is compromised by the absence of small subunits. Epimerization and isomerization of the substrate resulting from misprotonation of the enediol intermediate were not significantly exacerbated by lack of small subunits. The residual carboxylating activity partitioned product between pyruvate and 3-phosphoglycerate in a ratio similar to that of the holoenzyme, indicating that stablization of the penultimate three-carbon aci-acid intermediate is not perturbed by lack of small subunits. The underlying instability of the five-carbon enediol intermediate was revealed, even with the holoenzyme, under conditions designed to lead to exhaustion of substrate CO2 (and O2). When carboxylation (and oxygenation) stalled upon exhaustion of gaseous substrate, both spinach and Synechococcus holoenzymes continued slowly to beta  eliminate inorganic phosphate from and to misprotonate the enediol intermediate. With carboxylation and oxygenation blocked, the products of these side reactions of the enediol intermediate accumulated to readily detectable levels, illustrating the difficulties attendant upon ribulose-P2 carboxylase's use of this reactive species as a catalytic intermediate.


INTRODUCTION

The photosynthetic enzyme Rubisco1 (EC 4.1.1.39) catalyzes the addition of CO2 to ribulose-P2, producing two molecules of P-glycerate using a multistep reaction sequence involving at least three enzyme-bound catalytic intermediates (Scheme 1) (for reviews, see Refs. 1-3). Two of these intermediates, the first and the third, are strong nucleophiles by virtue of their enediol character. Both are susceptible to side reactions that abort the carboxylation sequence and compromise the catalytic efficiency of the enzyme.


Scheme 1. The catalytic sequence of Rubisco's carboxylation reaction illustrating the intermediates involved, their stereochemistry, and their propensities for side reactions. Solid arrows indicate the productive pathway, and dashed arrows show the side reactions. Me2+ is the essential divalent metal ion, normally Mg(II). R is -CHOH-CH2OPO32-.
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The first of these intermediates is the 2,3-enediol form of ribulose-P2 produced by removal, by an enzymatic base, of the proton attached to C-3 of ribulose-P2. There may be two or more differently protonated forms of this intermediate; only the enediol form is shown in Scheme 1. Collectively, we refer to all forms of this intermediate as "the enediol." This is the species that must be attacked by CO2 at C-2 and (concertedly or sequentially) by H2O at C-3 to form the six-carbon intermediate and thus allow carboxylation to proceed (Scheme 1). Three different classes of side reactions are known for this intermediate. First, the enediol is also attacked by O2 at C-2, resulting in the formation of 2-phosphoglycolate which is, in turn, partially recycled to photosynthetic metabolism by the photorespiratory glycolate pathway (4-6). Second, the enediol can be reprotonated incorrectly, producing pentulose bisphosphate isomers of the substrate (xylulose-P2 and 3-ketoarabinitol-P2) (7-12) which are strong inhibitors and/or very weak substrates of the enzyme (13-15). Third, mutants of Synechococcus PCC 6301 and Rhodospirillum rubrum Rubiscos were discovered that were compromised in their ability to stabilize the enediol. These mutant enzymes catalyzed the production of varying amounts of deoxypentodiulose-P as a result of beta  elimination of the phosphoryl group attached to C-1 of the intermediate (P1) (16, 17).

The final intermediate in the carboxylation sequence is produced following cleavage of the C-2/C-3 bond of the six-carbon intermediate (Scheme 1). This aci-acid form of the P-glycerate molecule, derived from carbons 1 and 2 of ribulose-P2 and the incoming CO2 molecule, requires stereospecific protonation at C-2 to form P-glycerate. Only a single kind of side reaction is known for this intermediate, beta  elimination of the phosphoryl moiety to produce enol-pyruvate and thus pyruvate (18). All wild-type Rubiscos studied so far partition approximately 0.7% of their aci-acid intermediate to pyruvate (18), and mutants are known in which this partitioning ratio is increased or decreased (14, 16, 17). Thus both of Rubisco's enediol-like intermediates are prone to beta  elimination of the P1 phosphate group, and there may be a common reason (16, 18). Stereoelectronic considerations suggest that the beta  elimination tendency is least when the beta  substituent is maintained coplanar with the enediol double bond. However, a single disposition of the C-1/bridge O bond cannot be found which satisfies this requirement for both intermediates because the double bond is differently positioned in the two intermediates. In order to maintain co-planarity with the double bond, the C-1/bridge-O bond must change its dihedral angle with respect to the C-2/carboxyl-C bond of the six-carbon and aci-acid intermediates through 90° following hydrolysis of the six-carbon intermediate (Scheme 1). This movement may not occur rapidly or completely enough to stabilize the aci-acid intermediate fully.

The ability to produce a small subunit-free version of Synechococcus Rubisco by expression of the cyanobacterial rbcL gene in Escherichia coli without the companion rbcS gene presents an opportunity for study of the influence of the small subunits on the catalytic mechanism. The large subunits assemble correctly into an octameric core that retains approximately 1% of the holoenzyme's kcat but with weaker substrate affinities (19). Although it is known that the L8 form distinguishes between CO2 and O2 as substrates similar to the L8S8 holoenzyme (20), information about the ability of L8 to suppress other side reactions is lacking. Such information would contribute toward understanding the way the small subunits influence the active-site structure to increase its activity and alter its kinetic properties. Because small subunits do not bind near the active site and make no direct contribution to its structure (21), such influences must be effected through indirect conformational changes. If the small subunit alters the way the P1 phosphate group is bound, as has been speculated from crystallographic structural information (22), then absence of the small subunit might be expected to perturb the ability to suppress beta  elimination side reactions.

In this study, we have assessed the extent of side reactions of the carboxylation sequence catalyzed by wild-type Rubiscos. We have avoided complications associated with the competing oxygenation pathway, itself prone to side reactions at least with mutant Rubiscos (23, 24), by excluding O2. In the case of the Synechococcus enzyme, we have studied both the L8S8 holoenzyme and its L8 core without small subunits. To amplify accumulation of side products derived from the enediol intermediate with both Synechococcus and spinach holoenzymes, we have used the device of allowing exhaustion of gaseous substrates to occur while a saturating concentration of ribulose-P2 still remains. Our data show that, even with wild-type Rubiscos, beta  elimination and misprotonation of the enediol intermediate are easily detectable and that beta  elimination of the enediol, but not other side reactions, is strikingly exacerbated by the absence of small subunits.


EXPERIMENTAL PROCEDURES

Materials

Ribulose-P2 (25), [1-14C]ribulose-P2 (26), and [carboxyl-14C]carboxypentitol-P2 (27) were prepared as described. NaH14CO3 was obtained from Amersham Corp. and o-phenylenediamine from Sigma. Rubisco holoenzyme (L8S8) expressed in E. coli from the rbcL and rbcS genes of Synechococcus PCC 6301 was purified as described (16). Spinach Rubisco was purified using a procedure involving polyethylene glycol precipitation followed by anion-exchange chromatography on a Waters Protein-Pak Q column, essentially as described by Edmondson et al. (7) but omitting the final gel filtration step.

Preparation of L8 Rubisco

E. coli HB101 harboring plasmid pDB53, which bears the Synechococcus PCC 6301 rbcL gene under the transcriptional control of the lac promoter (28), was used to produce large subunit octamers (L8) devoid of small subunits. L8 was extracted from E. coli, purified, and stored using the same procedures developed for the Synechococcus L8S8 holoenzyme (16). Immediately before use, L8 preparations were dialyzed overnight at 4 °C against 20 mM potassium phosphate buffer, pH 7.6, containing 1 mM dithiothreitol and concentrated using a Centricon 30 unit (Amicon).

Carboxylase Assay

The carboxylase activity of L8 Rubisco was routinely measured by 14CO2 fixation both in the presence and in the absence of added small subunits (19, 29).

Measurement of Pyruvate Production

Pyruvate was determined by measuring [14C]lactate formed during complete consumption of ribulose-P2 by Rubisco in the presence of 14CO2, lactate dehydrogenase, and NADH. The assay solution contained purified Synechococcus Rubisco (final concentrations, 150 µg·ml-1 of L8 or 5 µg·ml-1 of L8S8), 100 mM Epps-NaOH buffer, pH 8.0, 20 mM MgCl2, 0.1 mg·ml-1 of bovine serum albumin, and 20 mM NaH14CO3 (30 Bq·nmol-1). After preincubation for 10 min at 25 °C, 4 units·ml-1 of rabbit muscle lactate dehydrogenase (Boehringer Mannheim) and 0.2 mM NADH were added, and catalysis was initiated by adding 2 mM ribulose-P2. The assay solution was then incubated at 25 °C for a further 16 h. Unreacted NaH14CO3 was removed by adding H3PO4 to reduce the pH to 3.0 and vortexing for 5 min. Enzymes were then removed by filtration through Millipore Ultrafree-MC filters (10,000 nominal Mr cut-off), and a sample of the filtrate was chromatographed on a 5-µm Nucleosil C18 reverse-phase column (Macherey-Nagel, 0.46 × 25 cm) eluted at 0.9 ml·min-1 with 0.1% (v/v) aqueous trifluoroacetic acid (Merck, spectroscopic grade), pH 1.6, as the mobile phase. Fractions of 0.1 ml were collected and mixed with 3 ml of scintillant for scintillation counting. Pyruvate production was calculated as the ratio of 14C in lactic acid to the total 14C in lactic, glyceric, and 3-phosphoglyceric acids.

Analysis of the Products from [14C]Ribulose-P2

Experiments were conducted with Synechococcus L8S8 and L8 under anaerobic and CO2-saturating conditions. The enzymes were placed in the main compartment of a Warburg flask in 2 (L8S8) or 0.5 (L8) ml (final volume) of a solution containing 50 mM triethanolamine acetic acid buffer, pH 8.3, 20 (L8S8) or 8 (L8) mM magnesium acetate, and 40 mM NaHCO3. The L8 reaction also contained 10 mM K2HPO4 and 1 mM dithiothreitol. The phosphate was necessary to maintain solubility of L8 and its presence also necessitated the use of a lower Mg2+ concentration to avoid precipitation. Controls lacking enzyme and with decarbamylated L8S8 (minus Mg2+, plus 1 mM EDTA) were also included using otherwise the same conditions as for L8S8. The side arm of the flasks contained 0.59 µmol of [1-14C]ribulose-P2 (5.9 Bq·nmol-1). The flasks were installed in a Warburg apparatus, and an atmosphere of humidified 1% (v/v) CO2 in N2 was passed continuously through the flask during all stages of the experiment. After pre-equilibration with shaking for 30 min at 25 °C, the contents of the side arm were mixed with those of the main compartment to initiate the reaction. Shaking was stopped 20 s afterward, and incubation was continued for 2 h. To terminate the reaction, 0.1 volume of 0.5 M NaBH4 in 0.1 N NaOH (equilibrated under N2) was added via a septum-capped injection port, and the mixture was shaken for a further 30 min. Borohydride was discharged with Bio-Rad AG 50W-X8 resin (H+ form), and the sample was prepared for anion-exchange chromatography as described (16). A sample was applied to a 5-µm Spherisorb SAX column (0.46 × 25 cm, Alltech) and eluted with a phosphate gradient as described previously (16). Fractions comprising individual radioactive peaks were pooled, incubated with alkaline phosphatase, passed through Bio-Rad AG 1-X8 (formate) resin, and chromatographed on an Aminex HPX-87C column as described previously (16).

Additional experiments were conducted with Rubisco holoenzymes from spinach and Synechococcus under conditions designed to lead to exhaustion of CO2/HCO3- (and any traces of O2) while still leaving an excess of unreacted ribulose-P2. In this case, the enzymes were placed in the main compartment of the Warburg flask in 1 (spinach) or 0.5 (Synechococcus) ml (final volume) of a solution containing 92 mM triethanolamine acetic acid buffer, pH 8.3 (spinach) or 7.8 (Synechococcus), 23 mM magnesium acetate, 0.7 mM EDTA, 4 (spinach) or 3 (Synechococcus) mM NaHCO3, and 0.5 mg·ml-1 bovine carbonic anhydrase. Controls containing, in addition, 3 mM carboxypentitol-P2 were also included for each enzyme. The side arms of the flasks contained [1-14C]ribulose-P2 (6.4 (spinach) and 3.2 (Synechococcus) µmol (4 Bq·nmol-1)). The reaction mixtures were shaken for 15 min at 25 °C in a Warburg apparatus while an atmosphere of humidified 0.1% (v/v) CO2 in N2 was passed through. The flasks were then sealed before the contents of the side arm were mixed with those of the main compartment to initiate the reaction. Shaking was stopped 30 s later, and incubation was continued for 1 (spinach) or 3 (Synechococcus) h before termination. Termination and subsequent procedures were the same as described above for the saturating CO2 experiments.

For both CO2-saturating and CO2-limiting sets of experiments, the amounts of the different pentulose bisphosphates present at quenching were calculated from the amounts of radioactivity present in ribitol, arabinitol, and xylitol determined by chromatography on the Aminex HPX-87C column. The calculation assumes that the only bisphosphates present before reduction were those of ribulose, xylulose, and 3-ketoarabinitol, that reduction of ribulose-P2 produced the ribitol and arabinitol isomers in the same ratio as that observed in the minus-enzyme or plus-carboxypentitol-P2 controls, and that reduction of xylulose-P2 produced the arabinitol and xylitol isomers in equal amounts. Thus, [ribulose-P2] = (1 alpha )R; [xylulose-P2] = 2X; and [3-ketoarabinitol-P2] = A - alpha R - X, where R, A, and X are the concentrations of the bisphosphates of ribitol, arabinitol, and xylitol, respectively, present at quenching, and alpha  is the arabinitol/ribitol ratio observed in the minus-enzyme or plus-carboxypentitol-P2 controls. The value of alpha  averaged 0.76 in the present experiments.

Continuous Spectrophotometric Measurement of Deoxypentodiulose-P Formation

Deoxypentodiulose-P produced during catalysis by wild-type spinach and Synechococcus Rubiscos was measured continuously by exploiting the absorbance at 330 nm of the quinoxaline adduct with o-phenylenediamine. Reaction mixtures contained, in a total volume of 2 ml, 60 mM Epps-NaOH buffer, pH 7.9, 12 mM MgCl2, approximately 3 mM NaHCO3, 0.6 mM EDTA, 20 mM o-phenylenediamine, 0.5 mg·ml-1 of bovine carbonic anhydrase, and 3 to 4 mg·ml-1 of Rubisco. A control, lacking Rubisco, was also included. All components except the Rubisco were dissolved in N2-sparged solutions. The reaction mixture was placed in a septum-capped spectrophotometer cuvette, and the headspace was flushed with N2. After several minutes pre-equilibration at 25 °C, the reaction was initiated at time 0 by adding ribulose-P2 solution from a syringe to bring the final concentration to 6.1 mM. The ribulose-P2 solution had been neutralized to pH 6.8 and sparged with N2 just before use. The absorbance was monitored at 330 nm, and 20-µl aliquots were withdrawn with a syringe at intervals and added to 500 µl of 2% (w/v) perchloric acid at 4 °C. These samples were neutralized with K2CO3 and centrifuged, and aliquots of the supernatant were assayed for P-glycerate spectrophotometrically in a 2-ml assay solution containing 92 mM Epps-NaOH, pH 8.0, 18 mM MgCl2, 0.2 mM NADH, 50 mM phosphocreatine, 12 units of rabbit muscle creatine phosphokinase, 36 units of yeast P-glycerate kinase, 15 units of rabbit muscle glyceraldehyde-phosphate dehydrogenase, 36 units of rabbit muscle triose-phosphate isomerase, and 12 units of rabbit muscle glycerol-phosphate dehydrogenase (all enzymes from Boehringer Mannheim). P-glycerate content was measured from the reduction in absorbance at 340 nm which followed addition of ATP to 50 µM. Experiments with deoxypentodiulose-P produced by prolonged room temperature storage of ribulose-P2 at pH 8 (30) showed that the quinoxaline adduct is formed with a half-time of approximately 60 s under these assay conditions. Therefore, although adduct formation does induce a lag in these assays, it is not an unacceptably long one, given the slow rate of deoxypentodiulose-P formation and the long assay periods used. The amounts of deoxypentodiulose-P produced were calculated using the molar absorptivity of the quinoxaline adduct determined by measuring inorganic phosphate (31) released from chromatographically isolated adduct by alkaline phosphatase. The value obtained (9300 M-1·cm-1 in aqueous solution at pH 8) approximates those reported for other quinoxalines (32).

Protein Determination

Protein was determined with the Cu-bicinchoninic acid procedure (33) using Pierce reagents or by measuring the absorbance at 280 nm (34, 35). L8 concentrations were calculated from determinations of its carboxylase activity, assuming a specific activity of 50 nmol·min-1·mg-1 under the standard assay conditions where the bicarbonate concentration (25 mM) is approximately half-saturating for L8 (19).


RESULTS

Pyruvate Production by L8

The partitioning of Rubisco's aci-carbanion intermediate between its alternative products, P-glycerate and pyruvate (measured as lactate), was determined for both the L8 core and the L8S8 holoenzyme forms of Synechococcus Rubisco. In this study, a reverse-phase HPLC method was used to separate the lactic acid from the other labeled products. Three radioactive peaks were observed, corresponding to 3-phosphoglyceric acid, glyceric acid, and lactic acid (Fig. 1). Glycerate presumably is formed by dephosphorylation of a portion of the major product P-glycerate by traces of phosphatases contaminating the enzyme preparations. The large enzyme concentrations and extended assay periods necessitated by the feeble activity of L8 amplifies the effects of even tiny traces of phosphatases. No pyruvate (retention time 4.3 min) was apparent, confirming the efficacy of the coupling system. The partitioning ratios observed were 0.61 ± 0.05% (n = 6) and 0.71 ± 0.05% (n = 5) for L8 (Fig. 1A) and L8S8 (Fig. 1B), respectively. The value for the wild-type enzyme is in agreement with previous observations (16, 18).


Fig. 1. Reverse-phase HPLC separation of products formed by L8 (A) and L8S8 (B) Synechococcus Rubiscos in the presence of 14CO2, lactate dehydrogenase, and NADH. See "Experimental Procedures" for details. The elution of 14C during chromatography of the reaction products is shown aligned with the absorbance at 214 nm observed during separate chromatography of 3-phosphoglyceric, glyceric, and lactic acids standards. The filtered, acidified reaction mixture described under "Experimental Procedures" was diluted 4-fold with mobile phase before injection of 120 µl.
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Other Reaction Products of L8

Other side reactions catalyzed by L8 and L8S8 Synechococcus Rubiscos were examined by observing the products derived from [1-14C]ribulose-P2. Anaerobic, saturating-CO2 conditions were used. The labeled products were separated by anion-exchange HPLC after borohydride reduction (16). Partitioning between mono- and bis-phosphorylated sugar alcohols and P-glycerate for the L8S8 enzyme was the same as described previously (16) (Fig. 2A). For L8, a fourth radioactive peak eluting just after P-glycerate was also observed. Table I summarizes the distribution of radioactivity between the various peaks for both enzyme forms. The mono- and bisphosphate peaks from the L8 chromatogram were pooled and treated with alkaline phosphatase. The sugar-alcohol moieties were then chromatographed on a carbohydrate-separating column.


Fig. 2. Chromatography of the NaBH4-reduced products derived from [1-14C]ribulose-P2 in the presence of L8S8 (dashed lines) and L8 (solid lines) Synechococcus Rubiscos. See "Experimental Procedures" and Table I for details. A, anion-exchange HPLC of the reduced products. The peaks labeled mono-P, P-glycerate, and bis-P were identified by their co-chromatography with D-ribulose-5-phosphate, P-glycerate, and ribulose-P2, respectively. These standards were detected by their absorbance at 214 nm (not shown). B and C, Aminex HPX-87C chromatography of the pooled radioactivity eluting in the mono-P (B) and bis-P (C) fractions of the L8 chromatogram in A, after dephosphorylation. The refractive index traces indicate the elution of ribitol, arabinitol, and xylitol standards, labeled R, A, and X, respectively. U indicates unidentified radioactive peaks.
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Table I.

Partitioning of reaction products by L8 and L8S8 Synechococcus Rubiscos under saturating CO2 conditions

The data were obtained from a series of chromatograms similar to those shown in Fig. 2. The numbers in parentheses are the percentages that each product represents of the total products. See "Experimental Procedures" for further details.
Holoenzyme (L8S8)a L8

Conditions
  Rubisco protomers (µM) 0.4 20
  Initial HCO3- (mM) 40 40
  Initial ribulose-P2M) 293 1170
  Final bisphosphate (µM)b 5 59
  Reaction time (min) 120 120
Product analysis
  Deoxypentodiulose-P (µM)b 0  (0) 68  (6)
  P-glycerate (µM)b 279  (99) 966  (89)
  Unknown phosphorylated acidb 0  (0) 41  (4)
  Xylulose-P2M)c 1  (0) 12  (1)
  3-Ketoarabinitol-P2M)c 1  (0) 1  (0)

a  Fully carbamylated L8S8 in the presence of 20 mM Mg2+. Similar data were obtained with L8S8 under the experimental conditions required to maintain L8 solubility (8 mM Mg2+, 10 mM K2HPO4, 1 mM dithiothreitol, see "Experimental Procedures"). A control with decarbamylated L8S8 in the absence of Mg2+ gave results similar to those for the enzyme-free control.
b  Measured as the radioactivity eluting in the relevant regions of the anion-exchange chromatograms (Fig. 2), after subtraction if appropriate for the traces of radioactivity seen in the analogous regions in controls lacking enzyme.
c  Calculated as described under "Experimental Procedures."

Three peaks of radioactivity were obtained for the sugar-alcohol moiety derived from the monophosphate fraction co-chromatographing with the D-ribulose 5-phosphate standard. The middle peak was broader and larger than the flanking peaks, consistent with it being composed of two overlapping peaks (Fig. 2B). None of the peaks coeluted with either ribitol, arabinitol, or xylitol, the expected products of reduction of pentuloses. The elution pattern was similar to that obtained for the reduced sugar moiety of the monophosphate by-product seen with the T65V mutant form of Synechococcus Rubisco. This by-product was identified as deoxypentodiulose-P (16). The unknown peak eluting near P-glycerate (Fig. 2A) co-chromatographs with a peak produced nonenzymatically from [1-14C]ribulose-P2 under strongly alkaline conditions (data not shown) that we have tentatively identified as being composed of both isomeric, branched, carboxylic acids produced by benzylic acid-type rearrangement of deoxypentodiulose-P (30). If this assignment is correct, then the radioactivity in this peak should be summed with that in the deoxypentodiulose-P peak. Since the combined total concentration of deoxypentodiulose-P and its putative rearrangement products at quenching was many fold greater than the concentration of enzyme protomers (Table I), these compounds must be genuine products, not merely the consequence of release of enzyme-bound enediol intermediate on quenching.

Reduction and dephosphorylation of the bisphosphate fraction remaining after exposure of [14C]ribulose-P2 to L8 yielded all three pentitols together with a small unknown peak frequently seen in controls lacking enzyme (Fig. 2C). Ribitol and arabinitol predominated, suggesting that the consumption of ribulose-P2 was incomplete as might be expected from the poor affinity of L8 for ribulose-P2 (19). Furthermore, the arabinitol/ribitol ratio was similar to that observed following reduction of ribulose-P2 in the minus-enzyme control, suggesting that little if any 3-ketoarabinitol-P2 had been produced (Table I). A small amount of xylitol was also observed, but its amount indicates that only a trivial amount of xylulose-P2 had been produced (Table I).

By-products of Catalysis by Rubisco Holoenzymes Under Conditions Leading to Exhaustion of Gaseous Substrates

Under the usual CO2-saturating conditions, the Synechococcus holoenzyme produced negligible traces of by-products derived from the enediol intermediate, even when the reaction continued until the ribulose-P2 had been almost exhausted (Fig. 2A, Table I). In order to maximize the possibility of detecting enediol by-products with L8S8 holoenzymes, assay conditions were designed so that the gaseous substrates were exhausted well before complete consumption of ribulose-P2. The steady-state pool size of enzyme-bound enediol would be expected to be maximal under these conditions and, since carboxylating and oxygenating activities are suppressed by lack of substrates, the remaining ribulose-P2 would be exposed to large concentrations of enzyme for extended periods, amplifying the chance of detecting by-products. The data obtained (Table II) show that P-glycerate production ceased, as expected, when its concentration became approximately equal to the concentration of bicarbonate originally supplied. Given the high concentration of enzyme present, this would have occurred only seconds after initiation of the reactions. Subsequently, both spinach and Synechococcus holoenzymes converted the remaining ribulose-P2 to deoxypentodiulose-P and pentulose bisphosphate isomers of the substrate. Although production of these by-products was slow, readily measurable amounts had accumulated by the end of the experiments (Table II). While xylulose-P2 and 3-ketoarabinitol-P2 have been detected previously as trace products of spinach Rubisco (10-12), this is the first demonstration of the production of deoxypentodiulose-P by a wild-type Rubisco. In the case of the spinach enzyme, it is clear that deoxypentodiulose-P production represents true catalytic turnover because the amount of deoxypentodiulose-P produced far exceeded the concentration of active sites present. It is also possible that the deoxypentodiulose-P detected underestimates that actually produced because of further conversion of some of the deoxypentodiulose-P to other compounds, such as the rearrangement products mentioned above. These were not always detected as a discrete peak following the P-glycerate peak, as in Fig. 2A (solid line), and sometimes occurred as an unquantifiable tail on the P-glycerate peak (Fig. 2A, dashed line). For the Synechococcus holoenzyme, the deoxypentodiulose-P detected did not exceed the concentration of active sites present despite the longer incubation period.

Table II.

Partitioning of reaction products by spinach and Synechococcus Rubisco holoenzymes under conditions leading to exhaustion of CO2

The data were obtained from a series of chromatograms (not shown) analogous to those shown in Fig. 2. The numbers in parentheses are the percentages that each product represents of the total products. See "Experimental Procedures" for further details.
Spinach Synechococcus

Conditions
  Rubisco protomers (mM) 0.18 0.22
  Approximate initial HCO3- (mM) 4 3
  Initial ribulose-P2 (mM) 6.43 6.44
  Final bisphosphate (mM)a 1.87 3.28
  pH 8.3 7.8
  Reaction time (min) 60 180
Product analysis
  Deoxypentodiulose-P (mM)a 0.43  ( 8.0) 0.091  ( 2.3)
  P-glycerate (mM)a 4.13  (76) 3.07  (77)
  Xylulose-P2 (mM)b 0.64  (12) 0.50  (13)
  3-Ketoarabinitol-P2 (mM)b 0.21  ( 3.8) 0.31  ( 7.8)

a  Measured as the radioactivity eluting in the relevant regions of the anion-exchange chromatograms (eg. Fig. 4), after subtraction if appropriate of the traces of radioactivity seen in the analogous regions in controls with fully carboxypentitol-P2-inhibited Rubisco.
b  Calculated as described under "Experimental Procedures."

Deoxypentodiulose-P formation during catalysis by spinach and Synechococcus holoenzymes under conditions designed to lead to rapid exhaustion of gaseous substrate was also measured by observing the absorbance at 330 nm of the quinoxaline adduct formed between deoxypentodiulose-P and o-phenylenediamine (Fig. 3). For this experiment, it was important to exclude the possibility that other alpha -dicarbonyl species might also contribute to the absorbance at this wavelength. Two other alpha -dicarbonyl species are known to be produced by Rubisco side reactions. One is pyruvate which, like other alpha -keto acids (32), could form a quinoxaline adduct under acidic conditions. However, this possibility was excluded by a control experiment which showed that negligible increase in absorbance at 330 nm occurred when 0.5 mM pyruvate was added to this assay system at pH 7.9 (Fig. 3). Another is the alpha -dicarbonyl by-product of Rubisco's oxygenase reaction, detected with some mutants of R. rubrum Rubisco (23, 24). Use of strictly anaerobic conditions avoided this second potential complication in the present experiments.


Fig. 3. Continuous spectrophotometric measurement of deoxypentodiulose-P formation with wild-type spinach (solid line) and Synechococcus (dashed line) Rubiscos. Deoxypentodiulose-P and P-glycerate (open circle , spinach; bullet , Synechococcus) formation were measured as described under "Experimental Procedures" under conditions designed to lead to rapid exhaustion of gaseous substrates (total initial concentration of CO2 plus HCO3- plus O2, approximately 3 mM) while still leaving an excess of unreacted ribulose-P2 (initial concentration, 6.1 mM). Controls lacking enzyme (dotted line) and with ribulose-P2 replaced by 0.5 mM pyruvate (dotted-dashed line) were also included.
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For comparison, P-glycerate production was measured in small aliquots withdrawn at intervals from the cuvettes. For both enzymes, P-glycerate production was initially rapid but ceased abruptly when the P-glycerate concentration approached twice the concentration of NaHCO3 initially present, confirming CO2/HCO3- exhaustion (Fig. 3). This indicates that o-phenylenediamine did not seriously inhibit the carboxylase activity of either enzyme. The continuing rise in absorbance at 330 nm confirmed that deoxypentodiulose-P was indeed produced by both enzymes, supporting the conclusions from the analysis of 14C-labeled products (Table II) and establishing beyond doubt that this compound is a true by-product of both enzymes and not solely the result of degradation of enediol intermediate released at quenching. Using the molar absorptivity estimated for the quinoxaline adduct (see "Experimental Procedures"), it may be calculated from the absorbance increase that approximately 1% of the added ribulose-P2 had been converted to deoxypentodiulose-P by the spinach enzyme in 20 min. Allowing for differences in enzyme concentration and reaction time, this is in approximate agreement with the amount of this by-product seen in the product analysis experiments (Table II). No sign of a lag in the rate of absorbance increase was observed with either enzyme during the early period before CO2/HCO3- exhaustion. CO2/HCO3- obviously caused little suppression of deoxypentodiulose-P production, at least at the moderately low concentrations initially present in these experiments.

The rate of deoxypentodiulose-P production slowed progressively for both enzymes throughout the experiment (Fig. 3). For the spinach enzyme, this might be a result of inhibition caused by accumulating misprotonation by-products. Indeed, the kinetics of the decline for the spinach enzyme appeared quite reminiscent of the decline usually seen during catalysis which has been attributed to that cause (7-10). Synechococcus Rubisco, however, is not subject to progressive inactivation during catalysis, at least at CO2 saturation (16), and it showed a more pronounced decline such that, after 15-20 min, the rate of absorbance increase had fallen to approach the basal rate seen in the enzyme-free control. Therefore, accumulation of misprotonation products is not likely to be the cause of the inactivation in this case. Decarbamylation in the CO2-free conditions may be a more plausible reason.

The very slow increase in absorbance at 330 nm in the control lacking enzyme (Fig. 3) is consistent with known rate of spontaneous production of deoxypentodiulose-P from ribulose-P2 (30). Rapid conversion of the expected amount of ribulose-P2 to P-glycerate also engenders confidence that o-phenylenediamine does not quickly cause extensive ribulose-P2 degradation.


DISCUSSION

beta Elimination of the Enediol Intermediate Occurs Even with Wild-type Rubiscos

The discovery that mutagenic perturbation of Rubisco's active site in the region that binds the P1 phosphate of the substrate resulted in a tendency of the enediol intermediate to beta  eliminate the P1 group (16, 17) raised a question about whether this tendency existed at all with wild-type Rubiscos and, if so, whether it varied between different Rubiscos. Morell et al. (16) speculated that the improvement in catalytic effectiveness under limiting CO2 conditions that accompanied the evolution of higher plant Rubiscos might have been achieved by increasing the fraction of enzyme active sites in the enediol-bound form during steady-state catalysis. If so, any tendency to beta  eliminate the enediol intermediate would be more obvious with higher plant Rubisco than with bacterial or cyanobacterial enzymes which are adapted to higher CO2 concentrations. Experiments at saturating CO2 with wild-type Rubisco showed little sign of the beta  elimination product, deoxypentodiulose-P (Table I). Therefore, to address this question, it was necessary to devise experimental conditions where the wild-type enzyme could be exposed to ribulose-P2 for extended periods but prevented from disposing of it rapidly via carboxylation or oxygenation. This was achieved under anaerobic conditions by the simple expedient of ensuring that the starting concentration of ribulose-P2 was well in excess of the CO2/HCO3- concentration. With sufficient Rubisco present to exhaust the inorganic carbon supply (and any traces of O2) in seconds, residual ribulose-P2 would be left at a still saturating concentration for the remainder of the experiment. With the main routes of conversion of the enediol blocked by lack of gaseous substrates, the active site would thus be expected to remain maximally charged with enediol, maximizing its vulnerability to side reactions. This approach will only be successful if inactivation by decarbamylation does not occur quickly in the absence of CO2. For the spinach enzyme at least, the data of Edmondson et al. (8) provide confidence that decarbamylation is very slow under these conditions, providing ribulose-P2 remains saturating. This confidence is sustained by data which showed that deoxypentodiulose-P appeared in reaction mixtures containing both spinach and Synechococcus Rubiscos in easily detectable quantities under these conditions (Table II, Fig. 3) but not in a control with decarbamylated enzyme (Table I, footnote). Deoxypentodiulose-P formation was established both chromatographically, by resolving the products from [1-14C]ribulose-P2 (Table II), and spectrophotometrically, by measuring the formation of the quinoxaline adduct with o-phenylenediamine (Fig. 3). Both methods agreed that spinach Rubisco produced larger amounts of deoxypentodiulose-P than Synechococcus Rubisco. Either spinach Rubisco has a larger fraction of its active sites occupied by the enediol under these conditions, as speculated by Morell et al. (16), or it stabilizes or retains the enediol less effectively than Synechococcus Rubisco. An idea of the scale of deoxypentodiulose-P production can be obtained by comparing the amounts observed with those of xylulose-P2, previously shown to be produced by spinach Rubisco once in every 400 catalytic turnovers while a constant supply of CO2 was maintained (10). In the present experiments, spinach Rubisco produced approximately two-thirds as much deoxypentodiulose-P as xylulose-P2, whereas for the Synechococcus enzyme the ratio was approximately one-fifth (Table II).

As expected from previous studies (10-12), spinach Rubisco also produced considerable amounts of pentulose bisphosphate isomers under these conditions. Our data show that the wild-type Synechococcus holoenzyme produces these isomers also, and in approximately similar amounts (Table II). The method for calculating the contributions of xylulose-P2 and 3-ketoarabinitol-P2 to the pool of substrate isomers assumes that these are the only two isomers produced. A third isomer, 3-keto-D-ribitol-1,5-bisphosphate, is also a theoretically possible misprotonation product although not shown so far to be the product of any Rubisco. If it is present, the method for calculating the amounts of individual pentulose bisphosphates collapses because it provides a potential second source of ribitol and xylitol. Nevertheless, the conclusion that pentulose bisphosphate isomers of the substrate, in general, are produced remains unassailed.

Absence of Small Subunits Exacerbates beta  Elimination of the Enediol Intermediate, but Not of the aci-Acid Intermediate

The intrinsic tendency of the Synechococcus Rubisco holoenzyme to catalyze beta  elimination of its enediol intermediate is small enough to be overlooked in experiments at saturating CO2 because of very rapid conversion of all of the ribulose-P2 supplied to P-glycerate (Table I). In the absence of small subunits, however, the tendency becomes very large (Table I), rivalling that seen when Thr-65 in the P1 binding site of the large subunit was mutated (16). Thus it must be concluded that lack of small subunits also alters active-site geometry in ways that impair Rubisco's ability to bind or stabilize the enediol. This observation might provide some support for the idea that binding of the small subunits indirectly influences the arrangement of the P1-binding site within the active site (22). Alternatively, since lack of small subunits is known to weaken the affinity of the active site for ligands such as 2'-carboxyarabinitol-1,5-bisphosphate and to increase the Km (ribulose-P2) (19), increased beta  elimination might simply reflect a weakening of the active site's grip on the enediol with consequent decay of this intermediate after release into solution.

The impairment of the active site's ability to stabilize the enediol intermediate induced by absence of small subunits was not accompanied by a similar degradation of the ability to protect this intermediate from misprotonation (Table I). Nor was the stability of the aci-acid intermediate compromised by lack of the small subunits because L8 partitioned its carboxylated product between P-glycerate and pyruvate in a ratio similar to that of L8S8 (Fig. 1). Thus, although enolization and processing of the six-carbon intermediate are impaired to approximately equal extents by lack of small subunits (36), the impairment is quite specific in its effect on side reactions; only beta  elimination of the enediol is selectively stimulated. Similar specificity is not apparent with mutant Rubiscos with enhanced enediol beta  elimination. Where examined, these mutants of both Synechococcus and R. rubrum Rubiscos also show either enhanced misprotonation of the enediol and/or alterations in the amount of pyruvate produced (16, 17, 24, 37). The L8 data thus provide evidence that different features within the active site must be involved in stabilizing the enediol and aci-acid intermediates and that, even in the case of the enediol intermediate, different features are involved in suppressing its beta  elimination and its misprotonation.


FOOTNOTES

*   This work was supported by Australian National University's Center for Molecular Structure and Function. The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Dagger    Supported by an Australian Research Council Research Fellowship.
§   Currently Chargé de Recherches du Fonds National de la Recherche Scientifique (Brussels, Belgium). Present address: Centre d'Ingénierie des Protéines, Institut de Chimie, B6, Université de Liège, B-4000 Sart-Tilman (Liège 1), Belgium.
   To whom correspondence and reprint requests should be addressed: Research School of Biological Sciences, Australian National University P.O. Box 475, Canberra ACT 2601, Australia. Tel.: +61-6-249-5072; Fax: +61-6-249-5075; E-mail: John.Andrews{at}anu.edu.au.
1    The abbreviations used are: Rubisco, ribulose-P2 carboxylase/oxygenase; ribulose-P2, D-ribulose-1,5-bisphosphate; xylulose-P2, D-xylulose-1,5-bisphosphate; 3-ketoarabinitol-P2, 3-keto-D-arabinitol-1,5-bisphosphate; P-glycerate, 3-phospho-D-glycerate; deoxypentodiulose-P, 1-deoxy-D-glycero-2,3-pentodiulose-5-phosphate; carboxyarabinitol-P2, 2'-carboxyarabinitol-1,5-bisphosphate; carboxypentitol-P2, unresolved isomeric mixture of carboxyarabinitol-P2 and 2'-carboxyribitol-1,5-bisphosphate; L8, L8S8, large subunit octamer and holoenzyme of Rubisco from Synechococcus PCC 6301, respectively; kcat, substrate-saturated turnover rate; Epps, N-[2-hydroxyethyl]piperazine-N'-3-propanesulfonic acid; HPLC, high performance liquid chromatography.

Acknowledgments

We thank Nerida O'Shea for technical assistance and Gabriel Quinlan for preliminary measurements of pyruvate production by L8.


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