(Received for publication, April 4, 1997)
From the Laboratory of Lipoprotein Research,
Cardiovascular Institute, Mount Sinai School of Medicine, New York,
New York 10029, the
Department of Medicine, College of
Physicians and Surgeons, Columbia University, New York, New York
10032, ** The Kitasato Institute, Tokyo 108, Japan, and the
Department of Cell Biology, Harvard
Medical School, Boston, Massachusetts 02115
Apolipoprotein B (apoB) is the major protein component of atherogenic lipoproteins of hepatic origin. In HepG2 cells, the standard cell culture model of human hepatic lipoprotein metabolism, there is a limited availability of core lipids in the endoplasmic reticulum for association with nascent apoB. Under these conditions, apoB is partially translocated, interacts with cytosolic Hsp70, and undergoes rapid degradation. We show that increasing the expression of Hsp70 in HepG2 cells promotes apoB degradation. In addition, apoB is polyubiquitinated and its degradation both normally and after Hsp70 induction is blocked by inhibitors of the proteasome. The apoB that accumulates after proteasome inhibition is endoplasmic reticulum-associated and can be assembled into lipoproteins and secreted if new lipid synthesis is stimulated. Thus, apoB is the first example of a wild-type mammalian protein whose secretion is regulated by degradation in the cytosol via the ubiquitin-proteasome pathway. Furthermore, targeting of this secretory protein to the proteasome is regulated by the molecular chaperone Hsp70 and the availability of apoB's lipid-ligands.
Apolipoprotein B100 (apoB)1 is
essential for the assembly and secretion of very low density
lipoproteins from the liver, and the binding of apoB to the low density
lipoprotein receptor promotes the tissue uptake of low density
lipoproteins from the plasma (1, 2). The plasma level of apoB in humans
correlates directly with the risk of coronary artery disease (3, 4).
ApoB is a 540-kDa protein with a multidomain structure that includes a globular amphipathic domain at the amino terminus and several hydrophobic amphipathic sheet domains throughout its length (5, 6).
Like other secreted proteins, apoB is synthesized as a precursor
containing a signal peptide that targets the nascent protein for
translocation across the endoplasmic reticulum (ER) membrane (7).
Unlike most secretory proteins, however, a significant proportion of
newly synthesized apoB is degraded prior to secretion from hepatic
cells (8-10).
In HepG2 cells, a human hepatocarcinoma cell line, a large fraction of apoB undergoes rapid degradation, but if oleic acid (OA) is provided to the cells, there is stimulation of the synthesis of lipid components of apoB-containing lipoprotein particles, especially triglycerides, and protection of apoB from rapid degradation (10, 11). The relative increase in degradation when lipid is limiting is associated with an apparent slowing, or even halting, of translocation, resulting in a "bitopic" orientation of apoB in which some domains are exposed to the cytosol and some to the ER lumen (12-14). The protection of apoB by newly synthesized lipid may reflect a direct role in facilitating apoB translocation across the ER membrane or an indirect one by promoting the recently demonstrated (15, 16) association between apoB and microsomal triglyceride transfer protein (MTP), the ER-lumenal protein in liver required for the transfer of lipids to nascent apoB (17).
In its bitopic orientation, apoB associates with cytosolic heat shock protein 70 (Hsp70; 18), an abundant molecular chaperone which binds to unfolded proteins and facilitates their achieving native conformations or their translocation across membranes, including those of the ER (for recent reviews, see Refs. 19-22). In addition, recent studies have shown that Hsp70 and its cofactors participate in the degradation of certain proteins by the ubiquitin-proteasome pathway, by ATP-dependent proteases, and by lysosomes (23-25). Therefore, we set out to determine whether this chaperone may play a role in the degradation of nascent apoB. Because the protease inhibitor ALLN (acetyl-leucyl-leucyl-norleucinal; Refs. 26 and 27) protects apoB from degradation in HepG2 cells (e.g. Ref. 11), we also investigated the role of the ubiquitin-proteasome pathway in apoB degradation, given the recent demonstration that ALLN can inhibit protein breakdown by cytosolic proteasomes (28-30).
Herbimycin A (HA) and acetyl-leucyl-norleucinal (ALLN) were purchased from Sigma. ALLN was used at a concentration of 100 µM. MG132 was provided by ProScript, Inc. and used at a concentration of 10 µM. Lactacystin was either synthesized (31), provided by ProScript, Inc., or purchased from the laboratory of Dr. E. J. Corey, Department of Chemistry, Harvard University, and used at a concentration of 10 µM. The proteolytic inhibitors were dissolved in dimethyl sulfoxide; in control cell cultures, an equal volume of dimethyl sulfoxide ("buffer control") was added.
[3H]Leucine was used at a concentration of 150 µCi/ml
and was supplied as L-[4,5-3H]leucine from
Amersham with a specific activity of 159 Ci/mmol. For the experiments
in Fig. 3, [35S]methionine/cysteine was used at a
concentration of 100 µCi/ml and was purchased from NEN Life Science
Products as EXPRESSTM Protein Labeling Mix (specific activity >1000
Ci/mmol).
Cell Culture
HepG2 cells were grown as described in Ref.
10. Briefly, after seeding into collagen-coated plates, cultures were
maintained at 37 °C/5% CO2 in minimal essential medium,
10% fetal bovine serum (with penicillin and streptomycin to inhibit
bacterial contamination). The medium was changed every three days and
experiments were started after cells were 70-90% confluent. During
the experiments, cells were maintained at 37 °C/5% CO2
in serum-free medium with the indicated additions or treatments. In
Figs. 3 and 5, OA was provided as a complex with BSA (prepared as in
Ref. 10). The molar ratio of OA:BSA was 2:1 and the concentration
specified for the OA·BSA complex refers to the OA moiety.
In experiments in which HepG2 cells were transfected, the cells were treated with either Transfectam (Promega) alone (mock transfection) or Transfectam plus rat hsp72 cDNA. 48 h later, the cells were labeled for 2 h with [3H]leucine in the absence or presence of the indicated inhibitor (ALLN or lactacystin). Cell lysates and conditioned media were analyzed by immunoprecipitation with an anti-apoB antibody as below.
Immunoprecipitation and Immunofluoresence ProceduresThe
immunoprecipitations were performed under either nondenaturing (Fig. 1,
panel D; Fig. 2, panel A; Fig. 5, panel
A) or denaturing conditions (all other figures) and the
immunoprecipitates resolved by SDS-PAGE as described previously (18,
32). The antibodies used were mouse anti-human Hsp 72/73 (Boehringer
Mannheim), rabbit anti-human apoA-I (Calbiochem), rabbit anti-human
apoB made by us (15) or obtained from Calbiochem, goat anti-human albumin (Boehringer Mannheim), and rabbit anti-ubiquitin
(StressGen).
For immunofluorescence studies, cells were fixed with 3% paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with 1% BSA. The primary antibodies used were rabbit anti-human apoB (Calbiochem), mouse anti-human apoB (Caltag Laboratories), rabbit anti-human apoA-I (Calbiochem), mouse anti-58K protein (Sigma), and rabbit anti-calnexin (StressGen). The secondary antibodies were either Texas Red-conjugated goat anti-rabbit IgG or fluorescein isothiocyanate-conjugated goat anti-mouse IgG (Jackson ImmunoResearch Laboratories).
Fluorescent images were collected by a Nikon Optiphot-2 microscope connected to a Sony Digital Photo camera. The digitized images were printed using the Adobe Photoshop program.
The initial experiments used herbimycin A (HA), an ansamycin antibiotic that inhibits tyrosine kinases and markedly induces Hsp70 in several cell lines (33-35). Unlike heat shock or other injurious treatments that induce Hsp70, long term exposure to low doses of HA does not have any adverse effects on protein maturation, protein solubility, the integrity of the intermediate filament cytoskeleton, or overall cell viability (34). HepG2 cells were pretreated with HA for 0, 0.5, or 5 h, and ALLN was added to half of the cultures for the final 30 min of the 5-h period. All of the cultures were then incubated with [3H]leucine for 8 min and immediately lysed. HA did not appear to be toxic to the cells, since incorporation of [3H]leucine into albumin or total protein (trichloroacetic acid-insoluble material) was not changed (data not shown). As shown in Fig. 1A, treatment for 5 h, but not 0.5 h, resulted in markedly increased levels of Hsp70, as determined by immunoprecipitation with an anti-Hsp70 antibody. There was no effect of either HA or ALLN on the incorporation of [3H]leucine into immunoprecipitable apoB (Fig. 1, B and C). Since apoB intracellular degradation is negligible within an 8-min labeling period, these results indicated that neither HA nor Hsp70 affected the rate of apoB synthesis.
To test whether these treatments affected apoB degradation, a pulse-chase protocol was used. HepG2 cells, pretreated with HA and/or ALLN, were labeled with [3H]leucine for 15 min and then incubated without isotope for 40 min. For incubations in which ALLN was added in the pretreatment period, it was also present during the labeling and chase periods. Since rates of apoB synthesis were not altered by the treatments, and because minimal amounts of newly synthesized apoB are secreted in 40 min (10), the levels of radiolabeled apoB in the cell lysate at the end of the chase period reflected primarily intracellular degradation. As shown in Fig. 1, D and E, treatment with HA for 5 h, which markedly increased Hsp70 levels, resulted in significantly (p < 0.001) reduced recovery of apoB (lane 3 versus lane 1; bar 3 versus bar 1); thus, degradation of newly synthesized apoB was accelerated. This rapid degradation was inhibited by the addition of ALLN 30 min prior to the pulse labeling (lane 3 versus lane 4; bar 3 versus bar 4). In contrast, pretreatment with HA for only 30 min did not affect Hsp70 levels (Fig. 1A) and did not increase apoB degradation (lane 2 versus lane 1; bar 2 versus bar 1).
These consequences of HA treatment on apoB degradation were likely due to a direct effect of the induced chaperone. For example, after HA treatment, both Hsp70 levels and the association of Hsp70 with apoB were increased, as shown by co-immunoprecipitation of the two proteins with an anti-apoB antibody (Fig. 1D; lanes 3 and 4, lower bands). As noted above, HA appears to specifically induce Hsp70 in some cell types (35), but in others it can induce several heat shock proteins (34). To confirm that the results with HA were specifically due to the elevated level of Hsp70, HepG2 cells were either mock transfected or transfected with the cDNA for rat Hsp72. After a 2-h labeling period with [3H]leucine, apoB was immunoprecipitated from samples of cell lysate and conditioned medium. Hsp70 levels were significantly elevated following transfection and, consistent with the HA results, the recovery of apoB was decreased in the transfected cells, undoubtedly due to increased degradation, since ALLN blocked the effect of increasing the level of Hsp70 on apoB recovery (data not shown).
As noted earlier, when HepG2 cells are provided with OA, apoB translocation is favored and apoB degradation decreases, as does the extent of the association of Hsp70 with apoB (15, 18, 36). We therefore tested whether this protection from degradation would be affected by increased levels of Hsp70. HepG2 cells were treated with either OA alone, or with HA plus OA. After a 15-min incubation with [3H]leucine, cells were maintained in isotope-free medium, and labeled apoB in the cell lysate and medium was then measured at different times. As shown in Fig. 2, OA treatment significantly reduced apoB degradation, and, as a result, both cell and medium apoB levels were greater than in the untreated control wells (panels A and B). However, HA co-treatment, which markedly increased Hsp70 levels, decreased this protective effect of OA; thus, the amounts of both cell and medium-apoB in HA/OA-treated cells were less than those in OA-treated cells, but still more than those in control cells. As observed earlier (Fig. 1D), the greater apoB degradation observed in HA/OA-treated cells (compared with OA alone) was associated with increased apoB-Hsp70 complex formation (Fig. 2A, lower bands). In control experiments, treatment with HA for 4 h did not affect TG synthesis under either basal or OA-stimulated conditions, as measured by incorporation of [3H]glycerol into cellular TG (data not shown).
Taken together, these results suggest that elevated levels of Hsp70 increased the susceptibility of apoB to a degradative system, especially when lipid synthesis was not stimulated and apoB translocation was not favored. In addition, the enhanced apoB degradation due to increased Hsp70 levels was inhibited by ALLN, as is degradation under basal conditions (11).
Despite the important influence of degradation in regulating the net secretion of hepatic apoB-containing lipoproteins, the proteolytic machinery involved has not been identified definitively. A prime candidate is the proteasome, which has been shown recently to be the major site of degradation of cytosolic proteins in mammalian cells (28, 30). The proteasome is found in two major forms, a 20 S (700 kDa) and a 26 S (2000 kDa) particle. The 26 S form contains a 20 S particle associated with two 19 S (~600 kDa) regulatory complexes. The 20 S particle contains multiple peptidase activities and the 19 S complex provides the components necessary for the binding and ATP-dependent degradation of ubiquitinated proteins (30).
Several observations are consistent with a role for the proteasome in apoB degradation. 1) The breakdown of pre-secretory apoB is not reduced by chloroquine or ammonium chloride,2 which inhibit lysosomal proteolysis; 2) ALLN, although originally identified as an inhibitor of cysteine proteases, such as the calpains and cathepsins (26, 27), can also inhibit proteasomes (28-30); 3) apoB degradation requires ATP (37)3 as does the ubiquitin-proteasome pathway; and 4) recent studies suggest that Hsp70 and its co-factors (such as the DnaJ homologues) can participate in the degradation of other proteins by the ubiquitin-proteasome pathway (23, 25).
The hypothesis that the proteasome degrades apoB in HepG2 cells was tested by using inhibitors of the proteasome, MG132 and lactacystin, which are more potent and specific than ALLN (28, 30, 38-40). Like ALLN, MG132 is a peptidyl aldehyde that competitively inhibits the chymotrypsin-like and peptidyl glutamyl sites on the 20 S proteasome. Lactacystin is a natural product that covalently modifies the active site threonine of the 20 S catalytic site and does not affect any other known protease (30, 39). When used at the indicated concentrations for 2 h in the presence of [35S]methionine/cysteine, all three agents were comparably effective inhibitors of apoB degradation (Fig. 3B). Moreover, the radiolabeled apoB pool that accumulated was confined to the cell (Fig. 3A, top versus bottom). These data are consistent with the recent report that another peptidyl aldehyde, MG115, which weakly inhibits the proteasome, also decreased apoB degradation in HepG2 cells (41). Overall, then, the results in Fig. 3, A and B, suggest that apoB can be degraded by proteasomes in HepG2 cells and the reported effects of ALLN on apoB degradation (e.g. Refs. 11, 13, and 14) can be attributed to its inhibition of proteasomes and not other proteolytic activities.
We next investigated whether the apoB that accumulated when proteasome function was blocked could be secreted when lipid synthesis was stimulated. HepG2 cells were incubated as in Fig. 3A, but in some wells OA was added along with the proteasome inhibitors. A large fraction (typically over 50%) of the labeled apoB was recovered from the conditioned medium at the end of the experiment (Fig. 3C, bottom). In pulse-chase experiments, a similar result was obtained: cells were incubated for 15 min with [35S]methionine/cysteine in the presence or absence of lactacystin. This medium was then replaced by one that was isotope-free and the incubation continued for 20 min to allow for all of the incomplete radiolabeled apoB molecules to be translated into full-length proteins (8, 42). OA was then added to some wells, and aliquots of conditioned medium from the three treatment groups (no OA or lactacystin, lactacystin, OA + lactacystin) were collected for immunoprecipitation analysis at different times following OA addition. As shown in Fig. 3B, over the duration of the 150-min incubation with OA, the secretion of radiolabeled apoB synthesized in the presence of lactacystin was stimulated. These two experiments (Fig. 3, C and D) demonstrated that the apoB spared from degradation by the proteasome could be incorporated into lipoproteins and secreted if lipid synthesis was stimulated either during or after the translation of apoB. Similar results (i.e. the stimulation of apoB secretion) were obtained even when OA was added to lactacystin-treated cells as long as 2 h after the 15-min labeling period (data not shown). None of the inhibitors significantly affected the total protein content of the cells or the synthesis and secretion of total radiolabeled protein (n = 6 for each parameter). In addition, the cellular content of another apolipoprotein, apoA-I, which is the major protein of high density lipoproteins, was not significantly affected by proteasomal inhibition (n = 5).
We next investigated the cellular location of apoB that accumulated
during proteasomal inhibition. Cells were incubated with [35S]methionine/cysteine for 2 h and some cells were
treated with either MG132 or lactacystin. Cell lysates were centrifuged
at 4 °C and 100,000 × g for 1 h to obtain
microsomal (pellet) and supernatant (S100) fractions. Whatever the
treatment, over 80% of the labeled apoB was found typically in the
microsomal fraction (data not shown), suggesting that apoB protected
from degradation remained membrane-associated. Another approach was to
study the cells by immunofluorescence, which showed (Fig.
4) an accumulation of apoB in the cells after
proteasomal inhibition, in agreement with the subcellular fractionation
data and Fig. 3A. Without the inhibitor (Fig.
4A), most of the apoB signal appeared to be localized to the
Golgi, based on its appearance and its co-localization with the 58-kDa
Golgi-specific protein, but not with the ER-specific protein, calnexin
(data not shown). This was expected, since we have previously shown
that once apoB was transported to the Golgi, it was relatively stable
(43), whereas rapid degradation of apoB in the absence of inhibitor
would have resulted in a small steady-state pool of ER-associated apoB.
After inhibition of proteasomes (Fig. 4B), the apoB signal
was not only distributed more generally than in the untreated control
cells (Fig. 4A), but it also substantially overlapped with
the signal for calnexin (data not shown). These findings suggested that
pre-Golgi apoB was now stable and did not accumulate in the cytosol.
Moreover, these immunofluorescence results were specific for apoB,
since lactacystin treatment did not alter the distribution of apoA-I in
the cells (data not shown).
As noted earlier, the increased apoB degradation in cells with elevated levels of Hsp70 was inhibited by ALLN. To test more conclusively whether this degradation was also mediated by the proteasome, HepG2 cells were either mock-transfected or transfected with rat hsp72 cDNA, and the effects of lactacystin examined. Cells were radiolabeled with [3H]leucine for 15 min and incubated for 20 min in isotope-free medium. Transient transfection resulted in elevated Hsp70 levels (Fig. 5A, lanes 3 and 4). Hsp70 overexpression, however, did not affect albumin synthesis or secretion (data not shown). In contrast, radiolabeled cell apoB levels in the hsp72 cDNA transfected cells were approximately 50% lower than in the mock-transfected (control) cells (n = 3, p < 0.01; Fig. 5A, lane 3 versus 1; Fig. 5B, bar 3 versus 1). The addition of lactacystin to either the mock or hsp72 cDNA-transfected cells increased the total recovery of apoB by approximately 2- and 4-fold, respectively (Fig. 5A, lane 1 versus 2, lane 3 versus 4; 5B, bar 1 versus 2, bar 3 versus 4). Thus, both basal apoB degradation and that induced by high levels of Hsp70 were mediated by the proteasome.
Although some polypeptides may be directly degraded by 20 S
proteasomes, the selective elimination of many regulatory proteins and
proteins with highly abnormal conformations involves their covalent
conjugation to multiple ubiquitin molecules. This modification leads to
their rapid hydrolysis by the 26 S proteasome complex (30). We
therefore tested in HepG2 cells whether degradation of apoB involved
its ubiquitination. After a 1-h incubation with [3H]leucine, radiolabeled material with a higher
molecular weight than apoB was detected after cell lysates were first
immunoprecipitated with the anti-ubiquitin antibody, resuspended, and
then immunoprecipitated with the anti-apoB antibody (Fig.
6A, lane 2). That this material represented
apoB-ubiquitin conjugates was also supported by competition experiments
in which the addition of unlabeled apoB (in the form of low density
lipoprotein) to samples before the second immunoprecipitation step
reduced the recovery of labeled material migrating at or above the
expected position of apoB (Fig. 6A, lane 3). There was no
evidence for apoB-ubiquitin conjugates in the conditioned medium (data
not shown). Furthermore, when a 4% polyacrylamide gel was used to
better resolve high molecular weight proteins (Fig. 6B), treatment with lactacystin led to the accumulation of apoB-ubiquitin conjugates that migrated as a broad smear. This finding indicates that
apoB molecules were attached to polyubiquitin chains of different lengths. Thus, these data and a very recent report (41) suggest that
apoB in HepG2 cells is ubiquitinated prior to degradation by the 26 S
proteasome.
Overall, the results in this report show that in the standard
model of human liver lipoprotein metabolism, the HepG2 cell line, apoB
degradation is mediated by the proteasome and that Hsp70 participates
in this process. Although apoB is a secretory protein, and Hsp70 and
the proteasome are found in the cytosol, recent studies have shown that
proteasomes can degrade other proteins that are either secretory
(44-46) or transmembrane (29, 47-49). These examples, however,
primarily involve the degradation by the ubiquitin-proteasome pathway
of a non-secretory or mutant protein (e.g. CFTR (29) or
1-antitrypsin Z (45)). In contrast, apoB is a wild-type
mammalian secretory protein targeted to the proteasome as part of a
metabolic regulatory mechanism; i.e. degradation by the
ubiquitin-proteasome pathway is increased when lipid synthesis is low.
In this novel mechanism, the itinerary of a secretory protein is
regulated by the availability of its associated ligand (lipid). Such
regulation may serve a protective function by preventing the
accumulation of potentially toxic apoB aggregates in the ER when the
co-translational lipidation of apoB is inadequate; e.g. when
lipid synthesis or the activity of MTP is reduced.
The association of Hsp70 with apoB, which increases under conditions promoting degradation, and the enhanced degradation observed when Hsp70 is overexpressed indicate an important role for this chaperone in targeting apoB to the ubiquitin-proteasome pathway. ApoB tends to form irreversible aggregates in vitro and in vivo (50), especially when it is poorly lipidated. By binding to cytosolic domains in the non-native (unlipidated) state, Hsp70 could promote proteasomal degradation by maintaining aggregation-prone apoB in a conformation that makes it a better substrate for ubiquitin-conjugating enzymes or the proteasome itself. It is noteworthy, then, that the rapidly degraded CFTR variant is associated with Hsp70 (51). In addition, the Hsp70 cofactor Ydj1, a member of the DnaJ family of chaperones, is necessary in yeast for ubiquitination of certain unfolded proteins, while SIS, another family member, is essential for the subsequent hydrolysis of these substrates by the 26 S proteasome (23, 25).
A number of potential mechanisms exist for the targeting of ER-associated proteins to the 26 S proteasome (reviewed in Ref. 52): 1) direct access of components of the ubiquitin-pathway to a cytosolically exposed domain of the protein (e.g. Refs. 29 and 45); 2) retrograde transport of a partially or fully translocated protein from the ER to the cytosol (e.g. Refs. 44, 46, 48, 49, and 53); 3) accumulation of proteins in specialized regions of the ER from which vesicles are generated that contain proteins ultimately digested by the proteasome (54). In the latter two models, ubiquitination would presumably occur after a domain of the protein became exposed to the cytosol.
Regarding apoB, consistent with the first mechanism are data derived from HepG2 cells, McArdle RH7777 cells, and rat primary hepatocytes (12, 43, 55) that apoB has domains exposed to the cytosol. Also in support is a recent report in which an ALLN-sensitive protease degraded the COOH terminus of apoB, thereby generating a lumenal 70-kDa N-terminal fragment, which is secreted by HepG2 cells (13, 14).
Since apoB appears to be only partially translocated at the time it becomes susceptible to degradation, the second mechanism is also plausible, and could involve the retrograde movement of nascent apoB being facilitated by Hsp70. Directional movement of a protein across the ER membrane may be determined by its binding to chaperones or ligands outside the translocon, which would act as "rachets" (56-59). Thus, for apoB, association with lipids or MTP could promote forward movement (15), since both factors are known to facilitate apoB translocation, while Hsp70 binding to a cytosolic domain could promote retrograde movement, either directly or by favoring ubiquitin conjugation. This "tug-of-war" scenario would be consistent with the decreased level of apoB degradation observed when Hsp70 was induced in the presence versus the absence of OA (Fig. 2).
Either the direct access of the ubiquitin-proteasome machinery to, or the retrograde transport of, partially translocated apoB is compatible with the ability of HepG2 cells to secrete apoB spared from degradation when lipid synthesis was stimulated by OA after translation had been completed (Fig. 3D). By the time OA was added (20 min after the end of the 15-min labeling period), significant apoB degradation is readily detectable in HepG2 cells incubated in the absence of fatty acids and proteasome inhibitors (10). Notably, the apoB that accumulated in the presence of the inhibitor, but before OA addition, remained competent for translocation and assembly with lipids to form a lipoprotein. Thus, it is likely that apoB is associated with the ER membrane at the time it is attacked by the proteasome and neither dislocated to the cytosol, as can occur to MHC class I molecules in cells expressing either the CMV US2 or US11 gene product (48, 49), nor packaged into non-secretory vesicles (as are misfolded MHC class I molecules in mice deficient in the peptide transporter TAP (54)). Additional support for the ER association of apoB targeted for the proteasome comes from the results of our immunofluorescence (Fig. 4) and subcellular fractionation studies described above.
A diagram summarizing the major features of apoB degradation implied by
our data is shown in Fig. 7. Although additional studies are needed to define these features in greater detail, it is clear that
Hsp70 and the ubiquitin-proteasome pathway have the potential to
regulate the hepatic production of atherogenic lipoproteins.
We thank Dr. Ruben Mestril, University of California, San Diego, for the rat hsp72 cDNA plasmid and Jill F. Fisher for editorial assistance.