Identification of a Firefly Luciferase Active Site Peptide Using a Benzophenone-based Photooxidation Reagent*

(Received for publication, February 18, 1997, and in revised form, May 27, 1997)

Bruce R. Branchini Dagger , Rachelle A. Magyar Dagger , Karen M. Marcantonio Dagger , Kate J. Newberry Dagger , Justin G. Stroh , Linda K. Hinz and Martha H. Murtiashaw Dagger

From the Dagger  Department of Chemistry, Connecticut College, New London, Connecticut 06320 and  Central Research Division, Pfizer Inc., Groton, Connecticut 06340

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES


ABSTRACT

Firefly luciferase catalyzes the highly efficient emission of yellow-green light from substrate luciferin by a series of reactions that require MgATP and molecular oxygen. We prepared 2-(4-benzoylphenyl)thiazole-4-carboxylic acid (BPTC), a novel benzophenone-based substrate analog, intending to use it in photoaffinity labeling studies to probe the luciferase active site. Instead, we found that while BPTC was a potent photoinactivating reagent for firefly luciferase, it was not a photoaffinity labeling agent. Using proteolysis, reverse phase high-performance liquid chromatography, tandem high performance liquid chromatography-electrospray ionization mass spectrometry, and Edman sequencing, we identified a single luciferase peptide, 244HHGF247, the degradation of which was directly correlated to luciferase photoinactivation. Results of enzyme kinetics and related studies were consistent with this peptide being at or near the luciferin binding site. Further, peptide model studies and additional investigations on the nature of the photoinactivation process strongly suggested that BPTC catalyzed the formation of singlet oxygen at the active site of the enzyme. We describe here an uncommon example of active site-directed photooxidation of an enzyme by singlet oxygen.


INTRODUCTION

Bioluminescence is a captivating process in which living organisms convert chemical energy into light. Beetle bioluminescence, including perhaps 2000 species of fireflies (1, 2), has provided a fertile area of basic and applied research mainly focused on the North American firefly Photinus pyralis. As Equations 1 and 2 indicate, the key firefly luciferase enzyme functions as a monooxygenase, although it does so without the apparent involvement of a metal or cofactor. By some means, amino acid residues are recruited to promote the addition of molecular oxygen to luciferin which is then transformed to an electronically excited state oxyluciferin molecule and CO2, each containing one oxygen atom from molecular oxygen. The light emission process is extremely efficient; nearly a single photon is emitted per reacted luciferin molecule (3).
<UP>Luciferase</UP>+<UP>luciferin</UP>+<UP>ATP </UP><LIM><OP><ARROW>⇄</ARROW></OP><UL><UP>Mg</UP><SUP><UP>2+</UP></SUP></UL></LIM><UP> Luciferase · luciferyl</UP>−<UP>AMP</UP>+<UP>PP<SUB>i</SUB></UP> (Eq. 1)
<UP>Luciferase</UP> · <UP>luciferyl</UP>−<UP>AMP</UP>+<UP>O<SUB>2</SUB> ⇄ Luciferase</UP>+<UP>oxyluciferin</UP>+<UP>AMP</UP>+<UP>CO<SUB>2</SUB></UP>+<UP>h</UP>&ugr; (Eq. 2)

The luciferase-catalyzed reactions provide the basis for a wide variety of biochemical assays, many with important clinical applications (4, 5). Moreover, the luciferase gene (6) is widely used as a reporter in studies of gene expression and regulation (7). Additionally, the cloning and sequencing of P. pyralis luciferase and homologues from several beetles (8) have revealed that these enzymes are closely related to a large family of nonbioluminescent proteins (9, 10) that catalyze reactions of ATP with carboxylate substrates to form acyl adenylates.

The recent solving of the crystal structure of luciferase without bound substrates or ligands revealed an apparently unique molecular architecture consisting of two distinct domains (11); however, a detailed description of the luciferase active site remains elusive. Early chemical modification studies have failed to document the involvement of any specific amino acid residues in substrate binding or enzyme catalysis. Many of these studies have been hampered by the high nucleophilic reactivity of several of the luciferase cysteine sulfhydryl groups. However, the results of mutagenesis and other studies (12-14) substantiate the nonessential role of the luciferase thiols. Additional mutational studies (8, 15-17) have identified individual amino acids and regions of the protein, the modification of which alters the color of bioluminescence. No direct structure-function relationship has emerged to account for the color variation of firefly bioluminescence. We have recently used mutational analysis directed at the structural motif common to the superfamily of adenylate-forming enzymes to demonstrate that Ser-198 is directly involved in determining the Km of substrate MgATP, the overall pKa of bioluminescence, and the emission decay rate kinetics.1

In this study, we initially focused on characterizing the firefly luciferin binding site. We prepared BPTC,2 a novel benzophenone-based dehydroluciferin analog (Fig. 1). Dehydroluciferin is a potent reversible luciferase inhibitor (18) and a nonbioluminescent substrate for the adenylation reaction (Equation 1). We intended to use BPTC as a photoaffinity labeling reagent, fully expecting the benzophenone photophore (19) to be superior to azide-based reagents that we had previously investigated without success.3 We report here that BPTC is a potent photoinactivating reagent for firefly luciferase, but not a photoaffinity labeling agent. Instead, we will show that BPTC is a site-specific photooxidizing reagent that has enabled us to identify a luciferase active site peptide.


Fig. 1. Structures of firefly luciferin and related inhibitors.
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EXPERIMENTAL PROCEDURES

Materials

The following items were obtained from the indicated sources: firefly luciferase (crystallized and lyophilized powder from P. pyralis, EC 1.13.12.7), MgATP (equine muscle), Nalpha -p-tosyl-L-lysine chloromethyl ketone-treated chymotrypsin and sequencing grade trifluoroacetic acid (Sigma); HHGF (Macromolecular Resources). 4-Cyano-benzophenone (20) was prepared in two steps from 4-cyanobenzaldehyde and phenylmagnesium bromide by the procedure of Wagner and Siebert (21). HOPhHA was synthesized by a literature method (22).

4-Benzoylphenylthiocarboxamide

Hydrogen sulfide was bubbled for 2 h at room temperature into a stirred solution of 4-cyanobenzophenone (400 mg, 1.9 mmol) in anhydrous pyridine (2.5 ml) containing a few drops of triethylamine. Nitrogen then was bubbled through the solution to remove excess H2S, and the mixture was rotary evaporated leaving a yellow solid (400 mg, 1.7 mmol, 87%). The crude product was purified by flash chromatography (silica gel, acetone:hexanes, 30:70, v/v) to a yellow crystalline solid (240 mg, 1.0 mmol, 53%), m.p. 164-167 °C. IR (KBr) 3336 (s), 3137 (s), 1643 (s), 1425 (m), 1309 (m), 1270 (s) cm-1; 1H-NMR (Me2SO-d6, 250 MHz) delta  7.54-7.61 (m, 3H), 7.67-7.77 (m, 4H), 7.99 (d, 2H), 9.71 (s, 1H), 10.11 (s, 1H); 13C-NMR (Me2SO-d6, 63 MHz) delta  127.3, 128.7, 129.2, 129.7, 133.0, 136.7, 138.7, 142.8, 195.3, 199.3; high resolution mass spectrometry (fast atom bombardment) MH+.
<UP>C<SUB>14</SUB>H<SUB>11</SUB>NOS</UP>
<UP>Calculated:</UP> 242.0641
<UP>Found:</UP> 242.0672

Ethyl-2-(4-benzoylphenyl)thiazole-4-carboxylate

Ethyl bromopyruvate was added slowly to 4-benzoylphenylthiocarboxamide (510 mg, 2.1 mmol) in absolute ethanol (60 ml), and the solution was refluxed under N2 for 1.5 h. The mixture was rotary evaporated yielding a solid that was washed with cold 50% aqueous ethanol and dried overnight in vacuo at 50 °C. The product was obtained as a white solid (549 mg, 1.6 mmol, 78%), m.p. 100-102 °C. IR (KBr) 3118 (w), 2981 (w), 1726 (s), 1645 (s), 1603 (m), 1446 (m), 1288 (s) cm-1; 1H-NMR (CDCl3, 250 MHz) delta  1.42 (t, 3H, J = 7.1 Hz), 4.44 (q, 2H, J = 7.1 Hz), 7.44-7.66 (m, 3 H), 7.77-7.81 (m, 2 H), 7.87 (d, 2H, J = 8.6 Hz), 8.12 (d, 2H, J = 8.6 Hz), 8.21 (s, 1 H); 13C-NMR (CDCl3, 63 MHz) delta  14.4, 61.7, 126.8, 127.9, 128.4, 130.0, 130.7, 132.8, 136.0, 137.2, 139.1, 148.6, 161.3, 167.4, 195.8; high resolution mass spectrometry (fast atom bombardment) MH+.
<UP>C<SUB>19</SUB>H<SUB>15</SUB>NO<SUB>3</SUB>S</UP>
<UP>Calculated:</UP> 338.0852
<UP>Found:</UP> 338.0895

2-(4-Benzoylphenyl)thiazole-4-carboxylic Acid

To a solution of ethyl 2-(4-benzoylphenyl)thiazole-4-carboxylate (45 mg, 0.13 mmol) in ethanol (5 ml) was added 10% NaOH (1.7 ml). The yellow mixture was refluxed for 0.5 h, cooled to room temperature, and acidified to pH 3 (litmus) with 6 N HCl. A precipitate formed which was isolated by centrifugation, repeatedly washed with water, and dried in vacuo at 40 °C. Recrystallization from ethyl acetate provides a pure product (33 mg, 0.11 mmol, 81%), m.p. 220-222 °C (with decomposition). IR (KBr) 3300-2400 (br), 1695 (s), 1645 (s) cm-1; UV(NaPB) lambda max 256 nm (log epsilon  = 4.05) and 312 nm (log epsilon  = 4.31); 1H-NMR (Me2SO-d6, 250 MHz) delta  7.55-7.77 (m, 5H), 7.86 (d, 2H, J = 8.1 Hz), 8.13 (d, 2H, J = 8.1 Hz), 8.57 (s, 1 H), 13.28 (br s, ~1H); 13C-NMR (Me2SO-d6, 63 MHz) delta  126.5, 128.6, 129.6, 129.8, 130.6, 132.9, 135.7, 136.7, 138.3, 148.6, 162.0, 166.1, 195.0; high resolution mass spectrometry (fast atom bombardment) MH+.
<UP>C<SUB>17</SUB>H<SUB>11</SUB>NO<SUB>3</SUB>S</UP>
<UP>Calculated:</UP> 310.0538
<UP>Found:</UP> 310.0527.

Enzyme Assays

Luciferase activity was determined by a previously described peak height-based light assay method (14). Luciferase solution concentrations were determined in NaPB by absorbance measurements at 278 nm, epsilon  = 45,560 M-1 cm-1 (23). Luciferase adenylation activity with 0.1 mM luciferin or BPTC as substrates was assessed by measuring PPi generation at pH 7.8 and 8.6 using the Sigma Pyrophosphate Reagent.

Luciferase Photoinactivation Studies

Luciferase (0.5-0.6 mg/ml) in NaPB was mixed with BPTC (0.8-100 µM), and solutions were irradiated with a Rayonet 350 lamp (emitted light, 300-400 nm) at a distance of 7 cm. During photolysis, all samples were kept on ice under a Pyrex glass filter. In the standard photoinactivation protocol, no special precautions were made to remove O2. To exclude O2, NaPB solutions were bubbled with argon for 20 min and, in some cases, sodium dithionite (2.5 mM) was included.

Kinetic Constants

The reversible inhibition of luciferase by BPTC and HOPhHA was assessed with respect to luciferin and MgATP. For luciferin (6-40 µM), BPTC and HOPhHA levels ranged from 2.5 to 20 µM and 70 to 700 µM, respectively. For substrate MgATP (4-325 µM), BPTC and HOPhHA concentrations were 0.04-13.0 µM and 20-80 µM, respectively. Inhibitory constants (Ki) were estimated by linear least squares fits of data from initial velocity measurements performed in the dark. The data with each luciferase substrate were analyzed by the method of Dixon (24), and the Ki for MgATP was obtained using the method of Cornish-Bowden for uncompetitive inhibitors. An independent estimate of the BPTC binding constant was made from the irreversible photoinactivation data in Fig. 2. A plot of 1/kobsd versus 1/[BPTC] was used to determine Ki and k3 according to the equation 1/kobsd = Ki/k3[BPTC] + 1/k3 (24).


Fig. 2. Photoinactivation of luciferase by BPTC. Luciferase (8 µM) was irradiated at 4 °C in NaPB in the absence (black-square) or presence of BPTC: square , 100 µM; bullet , 16 µM; open circle , 8 µM; triangle , 4 µM; ×, 2.8 µM; down-triangle, 0.8 µM. At the indicated times, 4-µl aliquots were withdrawn and analyzed for enzyme activity (14). Inset, the observed first order rate constant kobsd as a function of BPTC concentration. The data points were fitted to a straight line by the method of least squares.
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Proteolysis and Analysis of Luciferase Peptides

BPTC-inactivated luciferase (5% activity) and control enzyme preparations (irradiated and dark) were digested with Nalpha -p-tosyl-L-lysine chloromethyl ketone-treated chymotrypsin (protease:protein, 1:20, w/w) in NaPB for 2 h at 25 °C and 3 h at 37 °C and then quenched by addition of soybean trypsin inhibitor. Chymotryptic peptides were separated by RP-HPLC (14); specific conditions are detailed in the figure legends. The peak associated with BPTC photoinactivation was collected from control and inhibited luciferase preparations (Fig. 3), concentrated by lyophilization, purified by rechromatography, and analyzed by LC/ESMS (14) and N-terminal sequencing (Biotechnology Support Facility, University of Kansas Medical Center).


Fig. 3. RP-HPLC separation of control and BPTC-inactivated luciferase peptides. Chymotryptic digests were prepared from luciferase (8 µM) irradiated in NaPB without (upper trace) and with (lower trace) 4 µM BPTC. Peptides (~75 µg) were separated at 40 °C by RP-HPLC on a Rainin C18 5-µm column (4.6 × 250 mm) eluted at a flow rate of 1 ml/min, using 0.1% aqueous TFA containing linear gradients of acetonitrile: 5% (v/v) for 5 min; 15% after 10 min; 25% after 20 min; 50% after 45 min; and 95% after 60 min. Inset, the designated (*) peaks from 250 µg of chymotrypsin digests of control and BPTC-photoinactivated luciferase samples were collected, lyophilized, and rechromatographed using 0.1% aqueous TFA with a linear gradient of acetonitrile: 10% (v/v) for 5 min; 12% after 10 min; 20% after 36 min; 90% after 56 min. The indicated peaks (#) for the control (upper trace) and photoinactivated samples (lower trace) were collected and analyzed by LC/ESMS (14) and Edman sequencing.
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Model Photooxidation Studies

Solutions of peptide HHGF, BPTC, and various additives were irradiated in NaPB at room temperature as described under "Luciferase Photoinactivation Studies." Aliquots were withdrawn and analyzed by RP-HPLC (14). Elution conditions were 40 °C at a flow rate of 1 ml/min, using 0.1% aqueous TFA containing linear gradients of acetonitrile: 10% (v/v) for 5 min; 20% after 20 min; and 75% after 60 min. HHGF concentrations were determined by peak integration. In a separate experiment, the products from irradiation of HHGF (200 µM) in the presence of BPTC (400 µM) were analyzed by LC/ESMS.


RESULTS

Synthesis and Characterization of BPTC

BPTC (Fig. 1) was prepared in three steps in 33% overall yield from 4-cyanobenzophenone. The synthetic route was modeled after the preparation of dehydroluciferin developed by White et al. (25). The BPTC structure was confirmed by high resolution mass spectrometry, IR, UV-visible, 1H- and 13C-NMR spectroscopy (see "Experimental Procedures"). The high molar absorptivity of BPTC solutions in NaPB (lambda max 312 nm, log epsilon  = 4.31) enabled photolysis experiments to be carried out with a Rayonet 350 nm source. Prolonged irradiation of BPTC up to 2 h did not alter its absorption spectrum. In the dark, using 0.1 mM BPTC, 6.0 ± 1.0 nmol of pyrophosphate/min/mg luciferase were released at pH 8.6. Thus, BPTC was a substrate for the luciferase-catalyzed adenylation reaction at pH 8.6 (although not at pH 7.8) as shown in Equation 1.

Irreversible Photoinactivation of Luciferase by BPTC

Irradiation of solutions of native luciferase (8 µM) and BPTC (0.8-100 µM) effected a time- and concentration-dependent inactivation of bioluminescence activity (Fig. 2). Identical results were obtained when recombinant enzyme was irradiated with several concentrations of BPTC. Equimolar and higher concentrations of BPTC rapidly inactivated luciferase. Interestingly, substoichiometric levels of BPTC also could fully inhibit the enzyme. The failure of gel filtration, dialysis, and incubation with dithiothreitol to regenerate enzyme activity provided further evidence for the irreversible nature of the photoinactivation process (data not shown). Additional control studies (Table I) demonstrated that luciferase retained full activity when irradiated in the absence of BPTC or when incubated in the dark with BPTC, affirming that the photoinactivation of luciferase required BPTC.

Table I. Controls and protective effects on BPTC photoinactivation of luciferase


Additions/conditionsa Half-lifeb % loss of activity

min
BPTC omitted 0
No irradiation 0
None 7.5 98
HOPhHA (20 mM) 75.0 25
MgATP (10 mM) 30.1 55
Argon bubbling 14.3 84
Sodium dithionite (2.5 mM) 301.0 7
NaPB-D2O (96%) 4.4 99
Superoxide dismutase (1 unit/ml) 7.5 98
Mannitol (10 mM) 7.5 98
Sodium azide (10 mM) 10.0 94

a Reaction mixtures containing 8 µM luciferase and 4 µM BPTC in NaPB were irradiated for 40 min as described under "Experimental Procedures."
b Half-lives were calculated from rates measured by monitoring loss of enzyme activity as described under "Experimental Procedures."

BPTC Inhibition of Luciferase Is Active Site Directed

Steady-state kinetic analysis (data not shown) of the effect of BPTC (without irradiation) on luciferase flash height generation demonstrated that BPTC was a very good reversible competitive inhibitor (Ki = 3.0 ± 0.3 µM) with respect to substrate luciferin (Km = 15 ± 4.0 µM) (26). The measured Ki value for BPTC is similar to that reported (18) for dehydroluciferin (Ki = 1.0 µM), the structure BPTC was designed to resemble. With respect to substrate MgATP (Km = 110 µM) (27), BPTC was found to be an uncompetitive inhibitor (Ki = 0.55 ± 0.05 µM). The BPTC Ki value was independently estimated to be 7.5 µM from analysis of the irreversible inactivation data presented in Fig. 2 (inset). Attempts to determine whether luciferin or known analogs could protect against BPTC photoinactivation were unsuccessful because these compounds absorb strongly in the irradiated region. To circumvent this problem, we prepared HOPhHA (Fig. 1), the absorption spectrum of which is transparent between 300 and 400 nm. With respect to luciferin and MgATP, HOPhHA was determined to be a reversible competitive inhibitor (Ki = 160 ± 20 µM) and an uncompetitive inhibitor (Ki = 9.0 ± 1.0 µM), respectively. HOPhHA (20 mM) was effective in preventing BPTC photoinactivation of luciferase by ~75% (Table I), indicating that a substantial portion of the enzyme modification is proximal to the luciferin binding site. A protective effect of only ~45% by MgATP (10 mM) was observed in a similar experiment.

Identification of a Photoinactivated Luciferase Active Site Peptide

RP-HPLC was used to separate proteolytic fragments generated from control and photoinactivated luciferase digested with chymotrypsin, trypsin, or endoproteinase Glu-C. Only chymotrypsin digests presented a pattern of peptides with any differences between control and inactivated samples, the sole difference being a ~75% decrease in the area of the peak indicated by an asterisk in Fig. 3. The decrease in area of this peak was directly correlated with the loss of enzyme activity (data not shown). However, no new peaks appeared in the digest of inactivated protein.

The peaks designated in Fig. 3 were further purified by rechromatography (Fig. 3, inset). By Edman sequencing and LC/ESMS, both peaks were found to contain two coeluting peptides, HHGF (MH+ = 497.4) and VRGPM (MH+ = 559.4), corresponding to regions 244-247 and 392-396 in the deduced luciferase sequence (6). The mole percentage of HHGF:VRGPM in the peak from the control sample was estimated from the sequencing data to be 78:22. In the photoinactivated sample, the HHGF content decreased ~70%, while the amount of VRGPM remained unchanged. Therefore, the loss of peptide 244HHGF247 was correlated to the BPTC-mediated photoinactivation process.

Model Photooxidation Studies with HHGF

The absence of any evidence for BPTC-peptide adduct formation suggested that no true photoaffinity labeling had occurred during the photoinactivation process. Instead, it seemed likely that photooxidation of one or both of the imidazole side chains of 244HHGF247 may be a major cause of luciferase inactivation. A model study was undertaken in which synthetic HHGF (200 µM) was irradiated with BPTC (400 µM). As evidenced by the analysis of the reaction products by LC/ESMS (Fig. 4), BPTC did catalyze the photooxidation of HHGF. Moreover, the formation of the putative products (Fig. 4, inset), assigned from the mass data and by analogy to reported studies (28, 29) on the dye-sensitized photooxidation of histidine, suggested that BPTC irradiation generates singlet oxygen. Additional model studies (Table II) supported the involvement of singlet oxygen: (i) sodium azide, a scavenger of singlet oxygen prevented degradation of HHGF; (ii) the use of buffers prepared in deuterium oxide, which prolongs the lifetime of singlet oxygen, accelerated HHGF photooxidation 3-fold; (iii) superoxide dismutase and mannitol, reagents that destroy superoxide and hydroperoxide radicals, respectively, had very little or no effect; and (iv) reduction of soluble oxygen by argon bubbling slowed the rate of HHGF photooxidation, while sodium dithionite, which removes soluble oxygen (30), nearly halted the process. None of these reagents affected HHGF photooxidation in the absence of BPTC.


Fig. 4. LC/ESMS analysis of HHGF photooxidation products. HHGF (200 µM) and BPTC (400 µM) in NaPB were irradiated at room temperature for 1 h. A portion (35 µl) of the mixture was analyzed by LC/ESMS (14), and the RP-HPLC chromatogram is shown. Inset, calculated and observed masses (MH+) for the putative products are tabulated. No assignable mass data were obtained for peaks 2 and 6, and peaks 8-10 corresponded to known system contaminants.
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Table II. Effects of oxygen depletion, quenchers, and solvent on the rate of BPTC-mediated photooxidation of HHGF


Additions/conditionsa Half-lifeb % loss of HHGFc

min
None 23.8 84
Sodium azide (10 mM) 0
NaPB-D2O (87%) 7.7 99
Superoxide dismutase (1 unit/ml) 17.8 90
Mannitol (10 mM) 24.3 82
Argon bubbling 38 64
Sodium dithionite (2.5 mM) 509.4 11

a Reaction mixtures containing 8 µM HHGF and 200 µM BPTC in NaPB were irradiated as described under "Experimental Procedures."
b Half-lives were calculated from rates measured by monitoring [HHGF] using RP-HPLC as described under "Experimental Procedures."
c Values calculated from RP-HPLC peak area measurements after 60 min of irradiation.

BPTC Photooxidizes Luciferase

The results of luciferase photoinactivation experiments in which soluble oxygen was reduced by argon bubbling and sodium dithionite indicated decreased or strongly suppressed enzyme inhibition (Table I). A control trial showed that sodium dithionite had no effect on BPTC stability. Therefore, unlike photoaffinity labeling experiments that are usually retarded by oxygen, BPTC-mediated photoinactivation required it. Since substoichiometric concentrations of BPTC completely inactivated luciferase (Fig. 2) and since BPTC was not consumed in the process (Fig. 3), the benzophenone derivative must function as a photooxidation catalyst. Although sodium azide only modestly protected luciferase against BPTC photooxidation, evidence supporting the involvement of singlet oxygen included (Table I): (i) a 1.7-fold decrease in the inhibition half-life measured in deuterium oxide buffers; and (ii) the failure of superoxide dismutase or mannitol to alter the inactivation rate.

Since Cys, Met, Tyr, Trp, and His are especially susceptible to photooxidation (31), we compared the content of these amino acids in irradiated control and BPTC-inactivated luciferase (Table III). A loss of His consistent with the determined level of 244HHGF247 photooxidation and a very minor decrease in Cys were the only differences noted (including all other amino acids not shown in Table III).

Table III. Partial amino acid analysis of luciferase irradiated with and without BPTC


Amino acid Predicteda Irradiated controlb BPTC inactivatedb,c

Cys 4 3.5  ± 0.2 3.0  ± 0.2
His 14 12.8  ± 0.1 12.0  ± 0.1
Met 14 19.7  ± 1.1 19.7  ± 1.1
Trp 2 1.9 1.5
Tyr 19 20.9  ± 1.0 20.6  ± 1.0

a Values deduced from the cDNA sequence (6).
b Values for Cys are averages based on duplicate thiol titrations (14). Trp values are from a single determination, and all others are averages based on four analyses by Commonwealth Biotechnologies.
c Residual activity was 8% of the irradiated control.


DISCUSSION

Active Site-directed Photoinactivation of Luciferase

Molecular modeling was used to design BPTC, a dehydroluciferin analog containing a benzophenone moiety (Fig. 1). BPTC very effectively inactivated luciferase irreversibly by a process requiring irradiation (Fig. 2). Furthermore, luciferase modification occurred at or near the luciferin binding site as: (i) BPTC was found to be a substrate for the adenylation reaction shown in Equation 1; (ii) the aryl ketone derivative was an effective reversible competitive inhibitor (Ki = 3.0 ± 0.3 µM) with respect to luciferin; and (iii) the BPTC-promoted inactivation process was effectively retarded by the luciferin analog HOPhHA (Table I). BPTC also was shown to be an uncompetitive inhibitor (Ki = 0.55 ± 0.05 µM) with respect to another luciferase substrate, MgATP. Since MgATP provided modest protection against photoinactivation, a portion of the luciferase inactivation may occur at a second site, possibly an allosteric binding site for ATP (32). Using proteolysis, RP-HPLC, LC/ESMS, and Edman sequencing (Fig. 3), we identified a single luciferase peptide, 244HHGF247, the degradation of which was directly correlated to luciferase photoinactivation. However, since complete enzyme inhibition was accompanied by the loss of only ~70-75% of the peptide, the BPTC-mediated photoinactivation process must occur at more than one site, possibly a cysteine residue. While HHGF was identified as the major site of luciferase modification by comparison to controls, no modified peptides were found. As suggested by model studies (Fig. 4), several modified HHGF peptides probably were formed, but no single one was produced at a measurable level.

Nature of the Photoinactivation Process

The modification of luciferase at 244HHGF247 could not, as expected for a benzophenone derivative, be associated with photoaffinity labeling because: (i) the photoinactivation process required oxygen; (ii) substoichiometric amounts of BPTC fully inactivated luciferase; (iii) no BPTC-HHGF adduct was detected; and (iv) BPTC was not consumed during the photoinactivation process. Instead, BPTC is a catalyst for luciferase photooxidation. There are many examples of substituted aryl ketones functioning as triplet sensitizers (35). In the BPTC structure, the extended conjugation provided by the para-substituted thiazole ring must shift the key electronic transition from n-pi * to pi -pi *, thereby producing a molecule much less reactive to H abstraction required for photoaffinity labeling (19). The extended conjugation in the BPTC structure also may make it a more efficient triplet sensitizer.

Although it is difficult to distinguish between type II (singlet oxygen) and type I (electron transfer between an excited triplet state and a substrate molecule) photodynamic processes, we propose that BPTC generates singlet oxygen at the luciferase active site in very close proximity to the peptide 244HHGF247. The results of model studies undertaken with synthetic HHGF (Table II; Fig. 4) strongly support our contention that BPTC is a sensitizer for singlet oxygen production. Likewise, evidence consistent with BPTC-promoted singlet oxygen inactivation of luciferase was compiled (Table I). However, two significant differences were noted between the enzyme and model studies: (i) azide ion was much less effective in protecting the protein; and (ii) the enzyme inactivation process was ~150 times faster than the photooxidation of the model peptide. Previously, Seliger and co-workers reported (33) the inactivation of luciferase with singlet oxygen generated by irradiating an immobilized Rose Bengal catalyst. They determined that luciferase was ~10 times more sensitive to singlet oxygen than histidine. The even greater sensitivity of luciferase to BPTC-photooxidation than the actual peptide being modified very likely reflects the close proximity of both oxygen and BPTC to the 244HHGF247 region. The failure of sodium azide to retard luciferase photooxidation in our study presumably reflects the inability of the ion to reach the enzyme site of singlet oxygen generation.

Possible Functional Role of 244HHGF247

To explain the greater sensitivity of luciferase to singlet oxygen, Seliger proposed (33) that a histidine residue was located at a putative enzyme binding site for molecular oxygen. Mutagenesis studies support a similar proposal for the importance of a histidine residue in oxygen binding in the bioluminescent photoprotein aequorin (34). Possibly, in the firefly protein, the region 244HHGF247 is involved in binding oxygen for subsequent addition to the luciferyl adenylate (Equation 2). BPTC binding near this site would be expected to make His-244 and/or His-245 the most susceptible residue(s) to photooxidation as we have observed. Alternatively, the tetrapeptide may be involved in luciferin binding. Interestingly, His-245 is conserved among all of the 13 known firefly luciferase sequences (8). Preliminary results of ongoing mutagenesis studies support an important functional role for His-245 since the mutant proteins H245Q and H245A show altered light emission kinetics and ~200- and ~400-fold lower relative activities, respectively, compared with wild-type luciferase. Furthermore, 244HHGF247 is located in the 226-250 region of the luciferase primary sequence in which minor changes produce dramatic shifts in the color of bioluminescence (8, 16). This region is part of the beta -sheet B subdomain, which is located in the large N-terminal domain of the luciferase structure (11). It appears that the tetrapeptide is located near an internal cavity containing ordered water molecules. The cavity is in a surface groove close to several invariant residues that comprise the putative luciferase active site (11). When the x-ray coordinates become available, it will be possible to better determine the location of the 244HHGF247 in the luciferase three-dimensional structure.

Concluding Remarks

We have described here an uncommon example of active site-directed photooxidation of an enzyme by singlet oxygen. At least one other example of site-directed photooxidation has been reported (36), although the active species responsible for oxidation of a Cys residue in Delta -3-ketosteroid isomerase was not determined. The benzophenone group, so useful in photoaffinity labeling studies (19), has been used here to efficiently catalyze the photooxidation of a histidine-containing firefly luciferase peptide. Potentially, BPTC and other substituted benzophenones with extended conjugation may prove generally useful as site-directed photooxidation reagents. We are currently investigating this possibility and extending the ongoing mutation studies to better determine the functional role of 244HHGF247 in firefly bioluminescence.


FOOTNOTES

*   This work was supported by the McCollum-Vahlteich Endowment and Pfizer Central Research. Portions of this work were presented at the Tenth National Conference on Undergraduate Research, Asheville, North Carolina, April, 1996.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Dagger    To whom correspondence should be addressed: Dept. of Chemistry, Connecticut College, 270 Mohegan Ave., New London, CT 06320. Tel.: 860-439-2479; Fax: 860-439-2477; E-mail: brbra{at}conncoll.edu.
1   Thompson, J. F., Geoghegan, K. F., Lloyd, D. B., Lanzetti, A. J., Magyar, R. A., Anderson, S. M. and Branchini, B. R., (1997) J. Biol. Chem. 272, in press.
2   The abbreviations used are: BPTC, 2-(4-benzoylphenyl)thiazole-4-carboxylic acid; HOPhHA, 6-(4-hydroxyphenyl)hexanoic acid; LC/ESMS, tandem high performance liquid chromatography-electrospray ionization mass spectrometry; NaPB, 0.1 M sodium phosphate buffer (pH 8.0); RP-HPLC, reverse phase-high performance liquid chromatography; TFA, trifluoroacetic acid. HHGF, histidylhistidylglycylphenylalanine; VRGPM, valinylarginylglycylprolylmethionine.
3   B. R. Branchini and M. H. Murtiashaw, unpublished results.

ACKNOWLEDGEMENTS

We thank Jeff Kiplinger and Richard Ware for providing the mass spectral analyses of the synthetic compounds and Evelyn Bamford for technical assistance.


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