Conformational and Functional Differences between Recombinant Human Lens alpha A- and alpha B-Crystallin*

(Received for publication, November 18, 1996, and in revised form, December 17, 1996)

Tian-Xiao Sun , Biplab K. Das and Jack J.-N. Liang Dagger

From the Center for Ophthalmic Research, Brigham and Women's Hospital, and the Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02115

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
Acknowledgment
REFERENCES


ABSTRACT

Human and other mammalian lens proteins are composed of three major crystallins: alpha -, beta -, and gamma -crystallin. alpha -Crystallin plays a prominent role in the supramolecular assembly required to maintain lens transparency. With age, the crystallins, especially alpha -crystallin, undergo posttranslational modifications that may disrupt the supramolecular assembly, and the lens becomes susceptible to other stresses resulting in cataract formation. Because these modifications occur even at a relatively young age, it is difficult to obtain pure, unmodified crystallins for in vitro experiments. alpha -Crystallin is composed of two subunits, alpha A and alpha B. Before the application of recombinant DNA technology, these two alpha -crystallin subunits were separated from calf lens in the denatured state and reconstituted by the removal of the denaturant, but they were not refolded properly. In the present studies, we applied the recombinant DNA technology to prepare native, unmodified alpha A- and alpha B-crystallins for conformational and functional studies. The expressed proteins from Escherichia coli are in the native state and can be studied directly. First, alpha A and alpha B cDNAs were isolated from a human lens epithelial cell cDNA library. The cDNAs were cloned into a pAED4 expression vector and then expressed in E. coli strain BL21(DE3). Pure recombinant alpha A- and alpha B-crystallins were obtained after purification by gel filtration and DEAE liquid chromatography. They were subjected to conformational studies involving various spectroscopic measurements and an assessment of chaperone-like activity. alpha A- and alpha B-crystallins have not only different secondary structure, but also tertiary structure. 1-Anilino-8-naphthalene sulfonate fluorescence indicates that alpha B-crystallin is more hydrophobic than alpha A-crystallin. The chaperone-like activity, as measured by the ability to protect insulin aggregation, is about 4 times greater for alpha B- than for alpha A-crystallin. The resulting data provide a base line for further studies of human lens alpha -crystallin.


INTRODUCTION

alpha -Crystallin is the major lens structural protein responsible for the supramolecular assembly necessary to maintain lens transparency. It is isolated as a large water-soluble aggregate with a mass of 800 kDa and is composed of two homologous subunits, alpha A and alpha B, each with a molecular mass of 20 kDa. With aging and cataract formation, alpha -crystallins gradually become a high molecular weight aggregate and eventually an insoluble protein (1). This is manifested by the observation that the major crystallin component in the insoluble protein fraction of aged and cataractous lenses is alpha -crystallin (2). The aggregation and insolubilization of alpha -crystallin are believed to arise from posttranslational modifications, which accumulate significantly because of the low turnover rate of lens crystallins. Two-dimensional gel electrophoresis indicated that human lens crystallins were modified extensively even at a relatively young age (3). The significance of the modification is that crystallins become partially unfolded, and this partially unfolding exposes hydrophobic surfaces and promotes hydrophobic interaction. In aged and cataractous lenses, aggregation and insolubilization take place because the concentration of partially unfolded protein is high.

Currently, most human lens specimens available for in vitro studies are from old human subjects or cataract surgery, and the crystallins, especially alpha -crystallin isolated from these specimens, are extensively modified. Therefore, it is difficult to obtain pure and unmodified human lens alpha -crystallin for in vitro investigations. An alternative is to use an animal model, but this option raises concern about whether the results are species-specific or can be extrapolated to humans. For these reasons, many investigators used recombinant DNA technology to clone lens alpha -crystallin (4-11). The technique was employed to prepare not only unmodified alpha -crystallin but also site-specific mutants to study the effect of site-specific mutation on protein conformation and function. These studies, however, dealt only with either alpha A-crystallin (4-8, 10, 11) or alpha B-crystallin (9). Moreover, no comparative study on conformation and function between recombinant alpha A- and alpha B-crystallin has been reported. In the present study, we prepared both recombinant human alpha A- and alpha B-crystallins and characterized their conformations by molecular spectroscopy. The results provide a base line for further studies of modification-related changes in protein interaction and conformation. In addition, alpha A- and alpha B-crystallins were recently detected in other tissues, such as heart, kidney, spleen, and retina (12-15). These findings raise question about the role or function of alpha A- and alpha B-crystallins in these tissues. Similar studies of the corresponding recombinant alpha A- and alpha B-crystallins from tissues other than the lens may yield information on their conformation and aggregation state. This information may provide a clue to their biological functions.


MATERIALS AND METHODS

Isolation and Cloning of alpha A- and alpha B-Crystallin cDNA

A complementary DNA library of HLE cells prepared with a ZAP-cDNA Synthesis Kit (Stratagene, La Jolla, CA) was generously provided by Dr. Toshimichi Shinohara. The alpha A gene (689 bp)1 and the alpha B gene (657 bp) were isolated by PCR using two specific primers for each. The forward primer, d(AAGCTG<UNL>CATATG</UNL>GACGTGACCATCCAGCACCC) for alpha A and d(CTAGCC<UNL>CATATG</UNL>GACATCGCCATCCACC) for alpha B, corresponds to a cDNA sequence containing an ATG translation start codon and incorporating a two-base change (AACATG right-arrow CATATG) for alpha A and a three-base change (ACCATG right-arrow CATATG) for alpha B to create an NdeI restriction site. The reverse primer for alpha A, d(GAC<UNL>CCTAAG</UNL>CTTTCCTGGCTGCTCTCTCAAACC), corresponds to the cDNA sequence 125 nucleotides downstream of the AAG stop codon and incorporates a HindIII site. The reverse primer for alpha B, d(TATTAGCTTGATAATTTGGGCCTGCCCTTAGC), corresponds to the cDNA sequence 89 nucleotides downstream of the UAG stop codon. The two restriction sites were created for the purpose of cloning alpha A or alpha B cDNA into the T7 expression vector pAED4, a 3322-bp ampicillin-resistant plasmid containing the restriction sites NdeI and HindIII at the polycloning sites (16). The primers were custom-synthesized by Life Technologies, Inc.

For PCR, we used a protocol for Pfu DNA polymerase supplied by the manufacture (Stratagene). The PCR mixture (10 ng of human lens epithelial cDNA, 200 pmol of each amplification primer, 10 µl of 10 × Pfu DNA polymerase buffer, 20 nmol each of four dNTPs, and 2.5 units of Pfu DNA polymerase in a final volume of 100 µl) was first heated to 95 °C for 1 min to denature the template DNA; it was then subjected to 30 cycles of 95 °C (45 s), 55 °C (1 min), and 72 °C (2 min) each. The PCR product was purified by 2% agarose gel electrophoresis and was double-digested with NdeI and HindIII. The expression vector pAED4 was similarly double-digested. The digested gene and vector were then ligated by DNA ligase. Escherichia coli strain NM522, which lacks the gene for T7 RNA polymerase, was transformed with 10 µl of ligation mixture. The transformed cells were spread on agarose plates containing ampicillin (100 µg/ml), and 10 clones were picked out. The expression construct was screened by double digestion with NdeI and HindIII to confirm the presence of the cDNA insert. The expression construct was propagated in E. coli strain NM522. The nucleotide sequence of the alpha A or alpha B cDNA in the construct was determined by Sanger sequencing; a fluorescence-tagged dideoxy termination method was used with an ABI Automatic Sequencing System (Brigham and Women's Hospital Automatic Sequencing and Genotyping Facility).

Expression and Purification of alpha A- and alpha B-Crystallins

For the overexpression of alpha A- and alpha B-crystallins, E. coli strain BL21(DE3) was transformed with expression constructs pAED4-alpha A and pAED4-alpha B, respectively. A colony was picked out from the freshly streaked plate and inoculated with 50 ml of LB containing ampicillin (50 µg/ml). The culture was grown to log phase (A590, 0.6-0.9). A 20-ml volume of the log phase culture was inoculated into 1 liter of LB containing ampicillin (50 µg/ml). The culture was incubated at 37 °C with vigorous shaking until the optical density reached 0.6-0.9 at 590 nm, at which point expression was induced by the addition of 1 ml of 1 M isopropyl beta -D-thiogalactoside. Incubation was continued for another 3 h. Cells were then harvested by centrifugation at 5,000 rpm for 10 min and kept at -80 °C. A control culture of E. coli BL21(DE3) cells with pAED4 was incubated and induced simultaneously.

The collected cells were suspended in TNE buffer (50 mM Tris-HCl, 1 mM EDTA, 100 mM NaCl) at a ratio of 3 ml of buffer to 1 g (wet weight) of cells, and 8 µl of 50 mM phenylmethylsulfonyl fluoride and 80 µl of lysozyme (10 mg/ml) per g of cells were added. The mixtures were kept at 4 °C for 20 min, after which deoxycholic acid (4 mg/g of cells) was added. After thorough mixing, DNase (1 mg/ml, 20 µl/g of E. coli cells) was added, and incubation at 37 °C was continued until the mixture was no longer viscous. By centrifugation at 12,000 rpm for 30 min, the cell lysates were separated into supernatant and insoluble pellets. The recombinant proteins were purified from the supernatant by the following successive steps: fractionation with 30-60% saturated ammonium sulfate, gel filtration on Sephacryl S-300HR, and ion exchange chromatography on DEAE-Sephacel. The purity of the recombinant alpha A- and alpha B-crystallins was confirmed with FPLC gel filtration, SDS-PAGE, and isoelectric focusing (IEF) gel electrophoresis, and the crystallin preparations were stored in 0.05 M phosphate buffer at -20 °C.

FPLC Gel Filtration

FPLC was carried out on a Superose-6 column (Pharmacia Biotech Inc. FPLC System) (17). The sample solutions were passed through a filter (pore size, 0.45 µm) before application to the column. The column was eluted at 0.3 ml/min. Gel filtration standards from Pharmacia were used for calibration.

SDS-PAGE and Western Blot

SDS-PAGE was performed in a slab gel (15% acrylamide) by the method of Laemmli (18) under reducing conditions. Electrophoresis was conducted with a constant voltage of 200 V for 1 h. The gels were stained with Coomassie Brilliant Blue R-250.

Proteins separated by SDS-PAGE were electrophoretically transferred to a nitrocellulose membrane with the Trans-Blot Transfer Cell (Bio-Rad). Transfer was carried out with low field current (30 V and 0.01 A) overnight. The detailed procedures were supplied by the manufacturer (Bio-Rad).

IEF

IEF was performed on the precast IsoGel agarose IEF gels (pH range, 3-10; FMC BioProducts, Rockland, ME) under either native or denatured conditions. Gels were stained with Coomassie Brilliant Blue R-250.

CD Measurements

CD spectra were measured with an Aviv Circular Dichroism Spectrometer (model 60 DS) (19). The reported CD spectra are the average of 5-10 scans, smoothed by polynomial curve fitting. The fit was checked with a statistical test so that the original data were not oversmoothed. The CD data were expressed as molar ellipticity in degrees cm2/dmol, with 115 as residue molecular weight.

Fluorescence Measurements

Fluorescence measurements were performed with a Shimadzu spectrofluorometer (model RF-5301PC). Trp fluorescence was measured with an excitation wavelength at 295 nm. The fluorescent probe, ANS, was used to study the hydrophobicity of protein molecules (20). The ANS (~50 µM) in alpha -crystallin (0.1 mg/ml in 0.05 M phosphate buffer, pH 7.6) was incubated at room temperature for 30 min before the measurements were made. The emission was measured with an excitation wavelength at 395 nm.

Chaperone-like Activity Measurements

Chaperone activity was assayed by aggregation of insulin. Aggregation was induced by dithiothreitol reduction of insulin B-chain in the presence and absence of alpha -crystallin. The procedures were similar to those reported by Farahbakhsh et al. (21), except that samples were prepared in a 96-well microplate. In brief, 0.4 mg of insulin was reduced with 10 mM dithiothreitol in the presence or absence of alpha -crystallin samples (0.05-1.6 mg) in a final volume of 250 µl. The microplate was read at 490 nm with a Bio-Tek EL-800 microplate reader at room temperature. The reading was recorded every 3 min until a plateau was reached. Observed optical densities were recorded and analyzed with the KC3 software provided by Bio-Tek.

Protein concentrations were determined with a Pierce bicinchoninic acid (BCA) assay (22).


RESULTS

Isolation and Cloning of alpha A- and alpha B-Crystallin cDNAs

alpha A- and alpha B-crystallin cDNAs amplified by PCR were analyzed on 2% agarose gel; alpha A cDNA (689 bp) showed a 0.7-kb band and alpha B cDNA (657 bp) showed a 0.65-kb band. After cloning into pAED4 and double digestion with NdeI and HindIII, the 1% agarose gel displayed a 3.3-kb band (3322-bp vector) and a 0.68-kb band (670-bp alpha A cDNA insert) for pAED4-alpha A and a 3.3-kb band (3322-bp vector) and a 0.60-kb band (607-bp alpha B cDNA insert) for pAED4-alpha B. A single digestion of pAED4-alpha A and pAED4-alpha B showed a single band of 4.0 kb (3322-bp vector plus insert). DNA sequencing of the 5'-end sequence of alpha A and alpha B cDNA in the expression constructs confirmed the correct reading frame.

Expression and Purification of alpha A- and alpha B-Crystallins

Human lens alpha A-crystallin (173 residues) and alpha B-crystallin (175 residues) were expressed in host E. coli BL21(DE3) using pAED4 expression plasmid. The isopropyl beta -D-thiogalactoside induction of pAED4-alpha A and pAED4-alpha B resulted in the expression of a polypeptide with an apparent molecular mass of 20 kDa, corresponding to the molecular mass of the alpha A and alpha B subunits (Fig. 1, lanes 2 and 4). After cell lysis, the water-soluble fractions of the lysates were used to purify recombinant alpha A-crystallin and alpha B-crystallins. The purified products were seen as a single band in SDS-PAGE and IEF gels (Figs. 1 and 2). Western blotting identified the alpha A-crystallin, but not alpha B-crystallin, with polyclonal anti-alpha antiserum (Fig. 3). This result may have been due to the low immunoaffinity for alpha B, as reported by Thomson and Augusteyn (23). The expression yield was about 30% of total bacterial protein, as estimated by FPLC profiles. alpha A- and alpha B-crystallins have almost the same apparent molecular mass, which is slightly lower than that of calf alpha -crystallin (700-800 kDa) (Fig. 4). This result indicates that recombinant alpha A and alpha B are properly folded and aggregated in E. coli. Their slightly smaller size may be due to the absence of modifications.


Fig. 1. SDS-PAGE of alpha -crystallins. Lane 1, standards; lane 2, crude recombinant alpha A-crystallin; lane 3, purified recombinant alpha A-crystallin; lane 4, crude recombinant alpha B-crystallin; lane 5, purified recombinant alpha B-crystallin; lane 6, human lens alpha -crystallin; lane 7, calf lens alpha -crystallin.
[View Larger Version of this Image (113K GIF file)]



Fig. 2. IEF of alpha -crystallins in native (A) and denatured (B) conditions. Lane 1, markers; lane 2, recombinant alpha A-crystallin; lane 3, recombinant alpha B-crystallin; lane 4, human lens alpha -crystallin.
[View Larger Version of this Image (37K GIF file)]



Fig. 3. SDS gel (A) and Western blot (B) of alpha -crystallins. Lane 1, prestained standards; lane 2, recombinant alpha A-crystallin; lane 3, recombinant alpha B-crystallin; lane 4, human lens water-soluble protein.
[View Larger Version of this Image (27K GIF file)]



Fig. 4. FPLC gel filtration profiles of alpha -crystallins on a Superose-6 column. The numbers at the top are the molecular weights of the markers. The flow rate is 0.3 ml/min.
[View Larger Version of this Image (23K GIF file)]


CD Measurements

The near-UV CD spectra of recombinant alpha A- and alpha B-crystallins are very different (Fig. 5). The difference may not arise solely from the different content of aromatic amino acids (Trp, Tyr, and Phe) but probably also involves a different tertiary structure; CD intensity is related more to the rotational strength of these residues than to their amount, and the rotational strength in turn depends on the compactness of the protein structure (24).


Fig. 5. Near-UV CD spectra of recombinant alpha -crystallins. The protein concentration is 1.5 mg/ml, and the cell path length is 10 mm. The CD ellipticity units are degrees cm2/dmol.
[View Larger Version of this Image (20K GIF file)]


The far-UV CD spectra are shown in Fig. 6; alpha B-crystallin displays a CD quite different from that of alpha A-crystallin. For alpha A-crystallin, the typical 218-nm band for beta -sheet conformation is apparent, and the position is shifted to a shorter wavelength for alpha B-crystallin. For a more precise estimate of the percentage of various secondary structures, we used the computer program SELCON provided by Greenfield (25), which is based on the work of Johnson (26) and Sreerama and Woody (27). The program utilized CD spectra of proteins for which the secondary structures had been determined by x-ray diffraction. Three structural components (alpha -helix, beta -sheet, and beta -turn) were obtained, with the remaining structure considered a random coil. The percentage of each secondary structure in this order is 5, 40, 16, and 39% for alpha A-crystallin and 14, 33, 18, and 35% for alpha B-crystallin. The figures agree well with previous reports that the major secondary structure of alpha -crystallin is beta -conformation (50-60%, beta -sheet and beta -turn combined) and that there is very little alpha -helical content (~5%) (28, 29); however, the present study indicates that alpha B-crystallin has a greater content of alpha -helix than alpha A-crystallin does.


Fig. 6. Far-UV CD spectra of recombinant alpha -crystallins. The protein concentration is 1.5 mg/ml, and the cell path length is 0.5 mm. The CD ellipticity units are degrees cm2/dmol.
[View Larger Version of this Image (19K GIF file)]


Trp and ANS Fluorescence

The Trp emission maximum provides a direct indication of whether the Trp residues (Trp-9 in alpha A-crystallin and Trp-9 and Trp-60 in alpha B-crystallin) are buried or exposed. Since all of these residues are all in the N-terminal domain (residues 1-63), which is hydrophobic and participates in high molecular weight aggregation, they should be relatively buried. alpha A- and alpha B-crystallin display emission maxima at 335 and 337 nm, respectively, which are close to that for calf alpha -crystallin (336 nm) (Fig. 7).


Fig. 7. Trp fluorescence of recombinant alpha -crystallins. The excitation wavelength is 295 nm, and the protein concentration is 0.1 mg/ml.
[View Larger Version of this Image (16K GIF file)]


ANS is a hydrophobic probe. Upon binding to protein hydrophobic surfaces, ANS fluorescence intensity increases, and the emission maximum shifts to a shorter wavelength, thus providing a sensitive measurement for probing protein hydrophobicity. Fig. 8 shows that ANS fluorescence is more than 3 times greater in alpha B- than in alpha A-crystallin, a difference indicating that alpha B- is more hydrophobic than alpha A-crystallin.


Fig. 8. ANS fluorescence of recombinant alpha -crystallins. The excitation wavelength is 395 nm. The protein and ANS concentrations are 0.1 mg/ml and 50 µM, respectively.
[View Larger Version of this Image (19K GIF file)]


Chaperone-like Activity

The protection of insulin from aggregation by calf alpha -crystallin has been extensively studied (21). We included calf alpha -crystallin for comparison in our study of recombinant alpha A and alpha B (Fig. 9). Clearly, alpha B-crystallin is far more effective than alpha A-crystallin in preventing insulin aggregation. In the protocol described under "Materials and Methods," 0.2 mg of alpha B-crystallin can completely protect insulin from aggregation, whereas 1.6 mg of alpha A-crystallin is required. Chaperone-like activity thus appears to correlate directly with protein hydrophobicity.


Fig. 9. Chaperone-like activity of alpha -crystallins. The insulin and dithiothreitol concentrations are 0.4 mg/ml and 10 mM, respectively. The numbers to the right of curves are the weight ratios of insulin to alpha -crystallin (mg/mg).
[View Larger Version of this Image (16K GIF file)]



DISCUSSION

Employing the DNA cloning technology, we have prepared pure alpha A- and alpha B-crystallins. The aggregation sizes of the pure preparations are slightly smaller than that of alpha -crystallin isolated from calf lens water-soluble fraction. It is not known whether this difference is due to the lack of modifications during folding and aggregation in E. coli, but alpha -crystallin does increase in size with development and aging. Conformational studies indicate that alpha A- and alpha B-crystallins have an amount of beta -conformation comparable with that of calf lens alpha -crystallin but that alpha B-crystallin has substantially more alpha -helical content, whose significance is not clear. The difference in tertiary structure, as manifested by the difference in near-UV CD (Fig. 5), may contribute to conformational stability. To our knowledge, no study of the difference in conformational stability between recombinant alpha A- and alpha B-crystallin has been reported.

The most striking difference between alpha A- and alpha B-crystallin is the greater hydrophobicity and thus the greater chaperone-like activity for alpha B- than for alpha A-crystallin. The significance of this finding is related to the facts that alpha B-crystallin is expressed in more tissues than alpha A-crystallin (13), that alpha B-crystallin is expressed at higher levels than alpha A-crystallin in some tissues (30, 31), and that alpha B-crystallin, like the small heat-shock protein, may function as a stress protein and may be expressed under various stress conditions and diseases (32-34). The greater chaperone function of alpha B-crystallin thus effectively stabilizes other proteins under stress and confers thermostability to cells. The mechanism for the chaperone-like activity of alpha -crystallin is interaction with other proteins partially unfolded by heat, denaturant, or other mechanisms such as the reduction of insulin (21, 35, 36).

The alpha A and alpha B subunits were conventionally separated under denatured conditions (e.g. in urea or guanidine HCl), and the alpha A- and alpha B-crystallins were reconstituted by removal of the denaturant (23, 37-39). The molecular weights of these reconstituted alpha -crystallins were always lower than that of native alpha -crystallin, indicating that the reaggregation is incomplete. The recombinant alpha A- and alpha B-crystallins thus provide the advantage that they are in the native form, which can be important to studies of the dynamic quaternary structure of alpha -crystallin. Previous studies with renatured alpha -crystallin (homopolymer and heteropolymer) indicated that the alpha A and alpha B subunits are structurally equivalent and interchangeable (23, 39). Such studies using recombinant alpha A- and alpha B-crystallins may yield more relevant data, since in vivo alpha A and alpha B subunits are interchanged under the native conditions after alpha A- and alpha B-crystallins are expressed and folded.

The biological significance of the 3:1 ratio of alpha A to alpha B subunits in the mammalian alpha -crystallin has not been established, although previous reports indicated that any ratios can be formed in vitro (23, 39). It is believed that the significance of the alpha A:alpha B ratio may be implied by the result of a comparative study on the conformational stability of alpha -crystallins formed by various ratios of alpha A and alpha B subunits. It is possible that alpha -crystallin with a 3:1 ratio of alpha A to alpha B is the most stable form.

A recent chaperone study used a site-specific mutation on alpha A-crystallin to study conformational change of the substrate bound to alpha -crystallin (40). A Trp-free alpha A-crystallin mutant was prepared, and Trp fluorescence of the substrate protein was probed during the chaperone action. The results, along with those of many previous recombinant studies (4-11), demonstrated the versatility of the recombinant DNA technology. During cataract formation, many posttranslational modifications, including nonenzymatic glycation, protein-protein and mixed disulfide formation, and photooxidation of Trp residues, were observed. Use of site-specific alpha -crystallin mutants may help us to understand modification-induced aggregation and insolubilization. For example, use of a Trp-free alpha -crystallin mutant in the UV irradiation studies may shed light on the role of Trp residues in lens damage due to sunlight. This and other studies mentioned above are under way in our laboratory.


FOOTNOTES

*   This work was supported by National Institutes of Health Grant EY 05803.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Dagger    To whom correspondence should be addressed: Center for Ophthalmic Research, 221 Longwood Ave., Boston, MA 02115. Tel.: 617-278-0559; Fax: 617-278-0556.
1   The abbreviations used are: bp, base pair(s); kb, kilobase pair(s); ANS, 1-anilino-8-naphthalenesulfonate; FPLC, fast performance liquid chromatography; IEF, isoelectric focusing; PAGE, polyacrylamide gel electrophoresis; PCR, polymerase chain reaction.

Acknowledgment

We are grateful to Dr. Toshimichi Shinohara for the gift of human epithelial cell cDNA library and for helpful discussion during the course of this study.


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