(Received for publication, September 23, 1996, and in revised form, November 11, 1996)
From the Institute of Human Nutrition,
Departments of Pediatrics and Physiology and Molecular
Biophysics, Columbia University College of Physicians and Surgeons,
New York, New York 10032 and § DuPont-Merck Research
Laboratories, Experimental Station,
Wilmington, Delaware 19880-0400
We have identified two yeast genes with similarity to a human cDNA encoding acyl-coenzyme A:cholesterol acyltransferase (ACAT). Deletion of both yeast genes results in a viable cell with undetectable esterified sterol (Yang, H., Bard, M., Bruner, D. A., Gleeson, A., Deckelbaum, R. J., Aljinovic, G., Pohl, T., Rothstein, R., and Sturley, S. L. (1996) Science 272, 1353-1356). Here, we expressed the human cDNA in the yeast double mutant, resulting in high level production of ACAT protein, but low in vivo esterification of ergosterol, the predominant yeast sterol. The activity of the human enzyme was increased by incubation of these cells with 25-hydroxy, cholesterol, an established positive regulator of mammalian sterol esterification. In contrast, the yeast enzymes were unaffected by this reagent. In vitro microsomal assays indicated no sterol esterification in extracts from the double mutant. However, significant activity was detected from strains expressing human ACAT when cholesterol was equilibrated with the microsomal membranes. The human enzyme in yeast utilized cholesterol as the preferred sterol and was sensitive to competitive (S58035) and non-competitive (DuP 128) ACAT inhibitors. The yeast esterifying enzymes exhibited a diminished sterol substrate preference and were sensitive only to S58035. Human ACAT had a broad acyl-CoA substrate specificity, the other substrate for this reaction. By contrast, the yeast enzymes had a marked preference for specific acyl-CoAs, particularly unsaturated C18 forms. These results confirm the yeast genes as functional homologs of the human gene and demonstrate that the enzymes confer substrate specificity to the esterification reaction in both organisms.
Free sterols are essential components of all eukaryotic membranes and exert a major influence on membrane fluidity and permeability and the activity of membrane-bound proteins (2). The esterification of sterol, i.e. the conjugation of fatty acids with sterols, plays an important role in sterol homeostasis, since it converts excess free sterol into a cytoplasmic storage form. In human cells, this esterification reaction is mediated by the enzyme, acyl-coenzyme A:cholesterol acyltransferase (ACAT (3)).1 ACAT activity has been localized to the rough endoplasmic reticulum and is present in all tissues tested (4). ACAT has several physiological functions with possible pathological consequences. In addition to maintaining intracellular cholesterol homeostasis, the ACAT reaction has been suggested to be involved in absorption of cholesterol from the intestinal lumen (5). Furthermore, the cholesterol ester synthesis rate in the liver may affect the secretion of very low density lipoprotein and thus plasma cholesterol and triglyceride levels (6). In adrenal cells, cholesterol ester is a sterol source for acute steroid hormone production. Most profoundly, the up-regulation of ACAT in macrophages and smooth muscle cells, accompanied by the accumulation of cholesterol ester in the arterial wall (foam cells), is an early event in the formation of an atherosclerotic plaque (7, 8).
Based on these functions of ACAT, pharmacological inhibitors of the esterification reaction have been proposed and developed as potential hypocholesterolemic and anti-atherogenic agents (9, 10). Such inhibitors could reduce cholesterol uptake in the intestine, increase the conversion of free cholesterol to bile acids in the liver, reduce very low density lipoprotein secretion, and decrease the accumulation of cholesterol ester in cells in the vascular wall. Despite the strong inhibitory effect of these compounds in vitro and in animal models, they have not been efficacious in lowering serum cholesterol in human trials (11-13). Whether the absorbable ACAT inhibitors may prevent foam cell formation is currently unknown. Sandoz 58035, one of the first specific ACAT inhibitors, is a competitive fatty acid homologue (14) while DuP 128 is a potent, non-competitive inhibitor of the reaction (IC50 of 10 nm (15, 16)). Compounds such as these, which inhibit mammalian ACAT by different mechanisms, may be useful in studying esterification in other organisms, and thus afford a more thorough overall understanding of the ACAT reaction.
The sterol esterification reaction is conserved from yeast to humans
(17). In each organism, the predominant sterol is structurally distinct. In mammalian cells, the major sterol source is cholesterol; in yeast, it is ergosterol. Microsomal preparations from rat liver exhibit marked specificity of the ACAT reaction for cholesterol. Notably, the reaction was very sensitive to changes in the sterol side
chain; the addition of a 24--methyl group, as in ergosterol, resulted in esterification at about 5% the rate of cholesterol. In
contrast, yeast microsomal preparations esterified both ergosterol and
cholesterol to similar extents (18). The predominant fatty acyl-CoA in
each species has not been as well studied. However, an order of
preference for the rat liver enzyme was established as oleoyl > palmitoyl > stearoyl > linoleoyl-CoA (19). Whether the ACAT
homologs in different species prefer one particular acyl-CoA substrate
over others is unknown.
A comprehensive understanding of the ACAT reaction and the further
development of useful ACAT inhibitors has been hampered by the
inability to biochemically purify the ACAT enzyme to homogeneity. A
human macrophage ACAT cDNA has recently been identified (20). By
homology searching with this sequence as a probe, we identified two
yeast ACAT homologs, ARE1 and ARE2 (for ACAT
related enzymes 1 and 2, respectively).2
Disruption of these genes produced a viable yeast cell with no detectable sterol ester (1). In the current study, we determined the
relative enzyme activities of Are1p and Are2p using a microsomal in vitro sterol esterification assay. Subsequently, we
successfully expressed a human ACAT cDNA in the
are1 are2
yeast
strain. This facilitated the comparison of sterol and acyl-CoA substrate specificities and inhibitor sensitivities of the yeast and
human enzymes.
Congenic yeast
strains with deletions in the yeast homologs of human ACAT,
ARE1 and ARE2, have been described previously
(1). Four strains of common genetic background (can1-100,
his3-11, 15, leu2-3, 112, trp1-1, ura3-1) were used: SCY059
(MAT, met14
HpaI-SalI, are1
NA::HIS3,
are2
::LEU2), SCY060 (MAT
, ade2-1,
are1
NA::HIS3), SCY061 (MATa, met14
HpaI-SalI,
are2
::LEU2), and SCY062 (MATa, ade2-1).
Transformation of yeast was performed with lithium acetate by amino
acid prototrophy selection (22). Yeast extract, Yeast Nitrogen Base,
Bacto-peptone, and Bacto-agar were from Difco; D-dextrose,
D-galactose, and D-raffinose were from
Sigma. Complete (YEPD), synthetic complete (SC), or SC
lacking uracil (SC-ura) media were prepared as described (23, 24).
Competent cells
of Escherichia coli strain DH5 (Life Technologies, Inc.)
and DNA modifying enzymes (Promega) were used according to the
manufacturers instructions. The human ACAT cDNA sequence was
obtained from a human leukocyte cDNA library by polymerase chain
reaction amplification as described (25). The polymerase chain reaction
fragment corresponding to the coding region flanked by NotI
and ApaI sites was subcloned into the pCMV vector
(Invitrogen) at the same sites. The pCMV vector containing ACAT was cut
with ApaI and made blunt ended by T4 DNA polymerase
treatment and then digested with NotI. The ensuing fragment
contains a full-length human ACAT open reading frame with the
translation initiation codon four bases away from the NotI
site. The yeast shuttle vector pRS-426-GAL was cut by SacI
and blunt ended with the Klenow fragment of DNA polymerase I and then
digested by NotI. The NotI, ApaI fragment containing ACAT and the linearized vector were ligated by T4
DNA ligase to construct the ACAT expression vector pRS426-ACAT. This
placed the ACAT open reading frame directly downstream of the yeast
GAL1/10 promoter (Fig. 1A).
SDS-Polyacrylamide Gel Electrophoresis and Immunoblotting Analysis
Yeast cells were grown overnight in SC-ura dextrose
(2%) liquid medium. Cells were harvested at mid-log phase and washed
with sterile water twice. The cells were resuspended in 10 ml of SC-ura medium containing 2% galactose and 1% raffinose and grown overnight. Cells were lysed by vortexing with glass beads in 0.01 M
borate, 0.15 M NaCl and resuspension of the low speed
pellet in 1% SDS, 5 M urea. Denaturing gel electrophoresis
(5 µg of total protein per lane) was performed using 12%
polyacrylamide for the resolving gel in the presence of 0.1% sodium
dodecyl sulfate (26). After SDS-polyacrylamide gel electrophoresis
separation, the proteins were electroblotted to nitrocellulose. The
membrane was blocked in 5% non-fat milk in 20 mM Tris-HCl,
137 mM NaCl, and 0.1% Tween 20 (TBST) and probed with 0.28 µg/ml of the DM10 ACAT antibody (27) in TBST, 1% non-fat milk for
1 h. Detection of the immune complexes was attained using
horseradish peroxidase-conjugated secondary anti-rabbit IgG antibody
and the ECL Western blotting detection reagent (Amersham).
Yeast strains containing pRS426-ACAT or vector control were grown in 5 ml of SC-ura (2% glucose) medium to a density of 107 cells/ml. The cells were harvested, washed twice with sterile water, and then resuspended in 10 ml of SC-ura media containing 2% galactose and 1% raffinose. For experiments to test the effect of 25-hydroxycholesterol on sterol esterification, 10 or 25 µg/ml 25-hydroxycholesterol in ethanol was added to the growth medium at this point. After 4 h, 1 µCi/ml [3H]oleate in tyloxapol/ethanol (1:1) was added and cells were pulsed for a further 4 h. Total lipids were prepared and analyzed as described (1). Incorporation of label into ergosterol ester was determined after scintillation counting and normalization to a [14C]cholesterol internal standard and the dry weight of the cells. The data is expressed as means of triplicate assays over at least two experiments with the corresponding standard deviations.
In Vitro (Microsomal) Sterol Esterification AssayYeast
cells containing pRS426-ACAT or the vector control were grown in 5 ml
of SC-ura media containing 2% glucose to a density of about
108 cells/ml. The cultures were diluted to 500 ml of SC-ura
media containing 2% glucose and grown overnight. The cells were
harvested, washed twice with sterile water, and then resuspended in 500 ml of ura-CM media containing 2% galactose and 1% raffinose and
allowed to grow for 6 h before freezing as a cell pellet. Frozen
yeast pellets were quick-thawed at 37 °C, washed in 2 × pellet
volumes of homogenization buffer (HB: 0.1 M potassium
phosphate, 0.5 mM EDTA, 1 mM glutathione, 20 µM leupeptin, 10 µg/ml benzamidine, and 2 mM phenylmethylsulfonyl fluoride) and spun at 2,000 × g to re-pellet cells. The supernatant was removed and
another 2 volumes of HB added to resuspend the cells. The suspension
was shaken with intermittent cooling in the presence of 1.0 g of
0.5-mm diameter glass beads in a mini-beadbeater (Biospec Products) at 5,000 rpm for 3 × 1-min intervals. The resulting homogenate was spun at 1,000 × g for 5 min and 15,000 × g for 15 min. The supernatant was removed and spun at
105,000 × g for 1 h to pellet the microsomes. This pellet was resuspended in ACAT buffer (0.1 M potassium
phosphate, 1 mM GSH, pH 7.4) and protein concentration
determined (28). Microsomes were aliquoted and frozen at 70 °C.
Rat liver microsomes were prepared as described previously (29).
Enzyme activity was determined by the rate of incorporation of [14C]oleoyl-CoA or [14C]cholesterol into steryl ester (29). The standard assay, in duplicate or triplicate, contained 200 µg of microsomal protein, 1 mg of bovine serum albumin, 20 nmol of oleoyl-CoA, and 20 µg of cholesterol in a final volume of 200 µl of 0.1 M potassium phosphate butter, pH 7.4, containing 1 mM glutathione. In control assays, labeled oleoyl-CoA or [14C]cholesterol was added after the reaction had been stopped. In assays where inhibitors were tested, the inhibitors were added in 5 µl of Me2SO and compared to a solvent control of 5 µl of Me2SO. S58035 and DuP 128 were used at a concentrations of 50 and 0.5 µM, respectively. For experiments on substrate preferences utilizing [14C]oleoyl-CoA (30,000 dpm/nmol), cold sterols were included whereas those utilizing [14C]cholesterol (40,000 dpm/nmol) used unlabeled acyl-CoAs. Where added, sterols were suspended in reaction buffer with the aid of Triton WR-1339 at a ratio of 30:1 (Triton/sterol, w/w) (29). After preincubation for 15 min at 37 °C, the reaction was initiated by the addition of oleoyl-CoA. The assay was stopped after 2.5 min by the addition of 4 ml of chloroform/methanol (2:1). Phase separation was induced by the addition of water (800 µl) and [3H]cholesteryl oleate (30,000 dpm) and 15 µg of cholesteryl oleate were added as the internal standard and carrier, respectively. The chloroform layer containing the lipids was dried under nitrogen and resuspended in 100 µl of chloroform for spotting on ITLC-SA thin layer plates (Gelman Sciences). Lipids were separated and quantitated as described above. The specific activity of ACAT was determined as picomoles of either cholesterol or oleoyl-CoA converted to steryl ester/min/mg of protein.
To initiate a molecular analysis of sterol esterification in the
eukaryotic micro-organism Saccharomyces cerevisiae (yeast), we identified two yeast gene products that exhibit significant structural and functional homology to the putative catalytic component of cholesterol esterification in human macrophages. We have shown that
deletion of these genes produces a yeast cell lacking sterol ester (see
Ref. 1 and Fig. 2). To confirm that these genes encode catalytic
components of the esterification complex we isolated microsomes from
wild type, single and double are mutant yeast
strains. The in vitro measurements of sterol
esterification essentially mirrored the assays performed in
vivo. Deletion of both ARE1 and ARE2
produces a strain devoid of microsomal ACAT activity. ARE1
encodes the minor isoform in terms of its contribution to
esterification, whereas Are2p is the major esterification enzyme (~80% of wild-type), in vitro and in vivo. The
activity of the yeast isoforms in wild-type cells was elevated
2-3-fold after equilibration of exogenous cholesterol into microsomes
prior to the assay (not shown). This increase in activity is similar to that observed in rat liver microsomes (29).
|
To compare and contrast the esterification activities conferred by the
yeast and human enzymes, we expressed a human ACAT cDNA originally
isolated from macrophages. The cDNA was fused to the divergent
inducible promoter from the yeast GAL1/GAL10 genes in pRS426-ACAT as described above (Fig.
1A). A conditional promoter (in this case
induced by galactose) was used to circumvent possible toxicity of high
level expression of human ACAT in yeast. In fact, no toxicity was
observed. The plasmid was used to transform ARE1
are2, are1
ARE2,
are1
are2
, and wild-type
yeast strains. Transformants were incubated overnight in SC-ura,
galactose/raffinose to induce expression of hACAT. Proteins were
prepared and analyzed by SDS-polyacrylamide gel electrophoresis and
immunoblotting with DM10 anti-ACAT antibody. A protein of approximately
48 kDa was detected under inducing conditions in the ACAT expressing
strain and not in the non-expressing strain (vector control, Fig.
1B). The size of the protein is smaller than the predicted
size of the ACAT primary translation product (63.8 kDa) but co-migrates
with proteins isolated from mouse adrenal microsomes, consistent with
previous reports (27, 30). The lower molecular weight species is most
likely a degradation product of the hACAT protein; it is detectable in
the mouse adrenal extracts on darker exposure (not shown), and is also
observed in preparations described elsewhere (27).
ACAT expressing strains were assayed in vivo for their
ability to esterify endogenous yeast sterols (primarily ergosterol). Despite the high level expression of hACAT protein there were no
alterations in incorporation of [14C]oleate into sterol
ester in wild type or single ARE mutant yeast cells.
However, in the are1
are2
double disruption strain expressing
pRS426-ACAT, a low but significant esterification activity was detected
at about 4% of a wild type yeast cell (Fig.
2A). The activity endowed by the hACAT
construct was further confirmed as genuine when it was observed to
increase in response to incubation of the cells with 25-OH cholesterol (Fig. 2B). Despite this, the level of activity was not
commensurate with the mass of protein observed in the immunoblot. It is
possible that the human ACAT expressed in yeast lost most of its
activity due to mis-folding or aberrant secondary modification of the
protein.
Another explanation of the low level in vivo activity of
hACAT expressed in yeast relates to the utilization of the preferred ACAT reaction substrate. In yeast, the predominant sterol is ergosterol which differs from cholesterol by the presence of unsaturation at the
C-7 position and at C-22, with an extra methyl group at C-24. It has
been shown that ergosterol is not a good substrate for rat liver
microsomal ACAT (18). To test if the human ACAT protein expressed in
yeast is functional and that the observed activity represents low
affinity of the human enzyme for ergosterol, we utilized an in
vitro assay system to measure ACAT activity (Table
II). The addition of exogenous cholesterol to the
microsomal assay greatly increased esterification by the hACAT
expressing strain approximately 6-fold. This suggests that one reason
for the low activity observed in vivo is the lack of
appropriate substrate for the mammalian enzyme. The in vitro
ACAT activity of microsomes from yeast expressing the hACAT was
sensitive to both competitive (Sandoz 58035) and non-competitive (DuP
128) inhibitors. Interestingly, the endogenous yeast enzymes were only
affected by the competitive inhibitor (S58035, Table II), even though
DuP 128 was present at 50 times its IC50 for mammalian
ACAT. This suggests that the binding site for DuP 128 is not conserved
between yeast and humans. To test if the individual yeast ARE enzymes
differ in their sensitivity to S58035, we prepared and assayed
microsomes from single are1 and
are2
mutants (strains SCY060 and SCY061).
Using a range of concentrations of the inhibitor, we estimated the
IC50 of Are1p and Are2p to be 31 and 34 µM,
respectively, whereas the IC50 of a preparation from wild
type cells was 20 µM. The IC50 of rat ACAT in
the same experiment was estimated as 40 µM.
|
Having demonstrated the activity of the human ACAT cDNA in yeast, we extended these observations to assess the affinity of the human and yeast enzymes for different sterol or acyl-CoA substrates (Tables III and IV). Despite the fact that the dominant sterol in yeast is ergosterol, the yeast ARE enzymes also readily esterified cholesterol. In contrast, human macrophage ACAT expressed in the double are mutant yeast showed a substrate selectivity similar to the mammalian enzyme as opposed to yeast, with little activity against ergosterol (Table III). Both yeast and rat enzymes esterified 7-dehydrocholesterol, while no apparent activity utilizing this substrate was observed with hACAT expressed in yeast.
|
|
To assess the acyl-CoA substrate specificity, the assay conditions were identical to those for sterol specificity except that activity was followed by the esterification of radiolabeled cholesterol in the presence of various unlabeled acyl-CoAs. Human ACAT expressed in yeast utilized various acyl-CoAs as substrates with palmitoyl, oleoyl, linoleoyl, and arachidonyl CoAs showing similar activity and stearoyl-CoA somewhat less. In contrast, yeast esterification was specific for C18 unsaturated fatty acids. Rat liver enzymes showed a broader specificity than either of the yeast enzymes, but in no case was activity against arachidonyl-CoA observed (Table IV).
Our previous demonstration of the in vivo requirement of the yeast ARE genes in sterol esterification provokes two hypotheses with regard to their role in this reaction. These proteins are homologous to a mammalian protein shown to be required for sterol esterification, in vitro (30). However, it was hypothetically possible that the ARE gene products mediate substrate transport to the rough endoplasmic reticulum, conventionally the site of esterification. A more parsimonious explanation would be that ARE1 and ARE2 encode isoforms of the catalytic components of this enzyme. We now find this latter possibility to be the case. The fact that microsomes from yeast strains deficient in ARE1, ARE2, or both genes display identical relative levels of in vitro activity to those observed in vivo, rules out a direct role for these proteins in sterol translocation. Furthermore, the substrate specificities of both the yeast and human enzymes expressed in yeast are most consistent with the ARE and hACAT proteins acting as catalytic components of the enzymatic complex as opposed to regulatory factors.
The observation of two enzymes for sterol esterification in yeast is
intriguing. The requirement for multiple enzymes for the same reaction
would be advantageous to a cell if one enzyme was either differentially
regulated, alternatively localized, or specific for a substrate. We are
currently developing reagents such as isoform-specific antibodies which
will enable us to address these issues. Presently, however, we are
incapable of determining if the observed difference in levels of sterol
esterification observed in are1 or are2
mutants represents different rate constants for the reaction or
different levels of protein expression. Clearly both enzymes are
active, since a null phenotype occurs only when both genes are
deleted.
To some extent the differences between the ARE isoforms and human
macrophage ACAT are as interesting as their similarities. Despite the
overall 49% identity at the protein level of Are1p and Are2p, the
proteins are quite divergent, particularly at the NH2-terminal regions. Similarly the yeast proteins show the
highest degree of conservation with human macrophage ACAT in the
COOH-terminal domain. Thus far, we have been unable to distinguish
Are1p and Are2p except in terms of their overall relative contributions to sterol ester mass. The human enzyme, however, is quite distinct. Yeast enzyme is neither induced by 25-hydroxycholesterol nor sensitive to the non-competitive ACAT inhibitor, DuP 128. However, the human enzyme demonstrates the predicted response to these reagents, namely
increased activity and inhibition, respectively. Since the
transcription of the hACAT cDNA is driven by a yeast promoter regulated only by carbon source, these responses, particularly the
induction by 25-hydroxycholesterol, clearly act at a
post-transcriptional point. This is in confirmation of previous studies
(30). The 71% inhibition observed with S58035 in wild type yeast could
be due to preferential inhibition of Are2p or to partial inhibition of
both isoforms. An analysis of microsomal preparations from single
are mutants refutes this latter hypothesis;
Are1p and Are2p were equally sensitive to the fatty-acyl analogue. Our
observations contradict those of Yu et al. (21), who suggest
that non-competitive inhibitors of the human ACAT reaction, also
inhibit the activity of the yeast enzymes. It should be noted that
these workers used the inhibitors at concentrations 3 orders of
magnitude higher than the observed IC50 for the human
reaction. We therefore conclude that the yeast enzymes differ at
whatever domain responds to these reagents. This is
consistent with the differences we observed in response to another
regulatory molecule, 25-hydroxycholesterol.
In addition to demonstrating the differences between the yeast and human reaction, our work also confirms the fidelity of the yeast expression system with which to study the human enzyme. It is clear that the expression of hACAT in yeast occurs at high levels in terms of mass and that the reaction proceeds in a similar manner to that performed in mammalian cells. Thus, it seems likely that the sterol substrate specificities and pharmacological inhibitions described here for recombinant hACAT, essentially reproduce those observed with the enzyme in its normal cellular context.
The acyl-CoA specificity profile is more complex. Under the assay conditions employed, the hACAT expressed in yeast utilized a much broader range of fatty acids for esterification than either the yeast or the rat liver enzyme (Table IV). This may suggest that the enzyme, although functional, is in a slightly different membrane environment, such that the active site is accessible by CoAs containing acyl chains of different chain lengths and desaturation. Species and tissue differences cannot be ruled out, since in this study, the properties of human macrophage ACAT are compared to rat liver ACAT. In previous studies, DuP 128 inhibition and sterol substrate specificity were similar between liver or macrophage preparations from human or rat.3 The yeast enzymes appear to be very specific for C18 unsaturated fatty acids; this is in agreement with previous work (31, 32) but different to that found by Taylor (33) who saw no significant changes in in vivo esterification with different fatty acid substrates. The effect of linoleic acid on the ARE proteins is difficult to assess, since normally this fatty acid is not present in yeast. The fatty acid specificity of rat liver ACAT in our study is similar to that observed previously with the exception of linoleic acid. Previous studies (19, 34), found an esterification rate for linoleoyl-CoA between 15 and 40%, the rate of oleate, while in our experiments they were equivalent. There are several possible explanations for the observed differences. The specific activity of ACAT in the earlier experiments was about 10-fold less than under present conditions and the corresponding assay times were much longer; 2.5 min versus 30-60 min previously. The specificity of steryl ester hydrolase could play a role over the longer assay period. This longer assay time could also affect the amount of fatty acid being incorporated into phospholipid, where linoleate was much preferred to oleate (19). In those experiments, the ratio of linoleate/oleate in sterol ester was low (0.15), however, the ratio in phospholipid was high (3.4) suggesting a preference for linoleate incorporation into phospholipid under these assay conditions. Furthermore, in the previous experiments, radiolabeled cholesterol was added as a tracer in acetone, while in our experiments, the cholesterol was equilibrated with microsomes using the detergent Triton WR-1339. The non-ionic detergent may facilitate the interaction of linoleoyl-CoA with the microsomal ACAT. The predominant mammalian hepatic sterol ester which reflects ACAT activity is cholesteryl oleate, but again this may be due to normally low concentrations of the essential 18:2 fatty acid or to alternate metabolism. In this regard, when a diet rich in linoleate was fed to African Green monkeys, the amount of hepatic cholesteryl linoleate increased by 3.3-fold (35).
The demonstration of distinct substrate specificities of the human reaction is an important observation. The relative occurrences of these substrates in different tissues and in different species will reflect both physiological and nutritional influences and is probably variable. The presence of multiple ACAT isoforms with substrate specificity or differential tissue-specific expression would confer greater flexibility to this critical homeostatic process, which could be considered in some organs to be a detoxification process. It is noteworthy that the human macrophage ACAT mRNA is most abundant in adrenal tissues but barely detectable in liver and intestine, and yet these latter tissues exhibit exceptionally high ACAT activity (36). Of interest, we have recently identified other ACAT related gene products in the data base of expressed sequence tags (1), which may be candidates for isoforms that mediate sterol esterification in specific tissues or cell types, possibly with alternate substrate specificities. The study of these isoforms in the yeast are mutant background is currently being performed in this laboratory.
We thank J. J. Rich for a critical reading of the manuscript and Richard Deckelbaum and Peter Gillies for support and encouragement. We are grateful to Dr. T-Y. Chang for provision of the DM10 antisera and Scott Schissel and Ira Tabas for the gift of mouse adrenal protein preparations.