Diversity of the Escherichia coli Type 1 Fimbrial Lectin
DIFFERENTIAL BINDING TO MANNOSIDES AND UROEPITHELIAL CELLS*

(Received for publication, December 17, 1996, and in revised form, April 22, 1997)

Evgeni V. Sokurenko Dagger , Veronika Chesnokova Dagger §, Ron J. Doyle and David L. Hasty Dagger par **

From the Dagger  Department of Anatomy & Neurobiology, University of Tennessee, Memphis, Tennessee 38163, the  Department of Microbiology and Immunology, University of Louisville, Louisville, Kentucky 40292, and the par  Research Service (151), Veterans Affairs Medical Center, Memphis, Tennessee 38104

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES


ABSTRACT

Type 1 fimbriae are the most common adhesive organelles of Escherichia coli. Because of their virtual ubiquity, previous epidemiological studies have not found a correlation between the presence of type 1 fimbriae and urinary tract infections (UTIs). Recently it has become clear that type 1 fimbriae exhibit several different phenotypes, due to allelic variation of the gene for the lectin subunit, FimH, and that these phenotypes are differentially distributed among fecal and UTI isolates. In this study, we have analyzed in more detail the ability of isogenic, recombinant strains of E. coli expressing fimH genes of the predominant fecal and UTI phenotypes to adhere to glycoproteins and to uroepithelial cells. Evidence was obtained to indicate that type 1 fimbriae differ in their ability to recognize various mannosides, utilizing at least two different mechanisms. All FimH subunits studied to date are capable of mediating adhesion via trimannosyl residues, but only certain variants are capable of mediating high levels of adhesion via monomannosyl residues. The ability of the FimH lectins to interact with monomannosyl residues strongly correlates with their ability to mediate E. coli adhesion to uroepithelial cells. In this way, it would be possible for certain phenotypic variants of type 1 fimbriae to contribute more than others to virulence of E. coli in the urinary tract.


INTRODUCTION

Escherichia coli is a commensal inhabitant of the mammalian large intestine and the most common cause of urinary tract infections (UTIs)1 in humans (1, 2). A variety of the so-called urovirulence factors may be important in enabling E. coli to become established in a urinary tract niche (1, 2), but the array of specific genetic factors found in urinary tract isolates of E. coli vary such that no single factor can be considered essential. Fimbriae (3), or pili (4), of E. coli enable the bacteria to attach to mucosal surfaces and have long been considered to be primary urovirulence factor candidates (1, 2). The role of P fimbriae in pyelonephritis is well-established, due in large measure to accumulated epidemiological evidence showing that approximately 70% of E. coli strains from pyelonephritis, but less than 20% of normal intestinal isolates, produce P fimbriae (1, 2, 5). Still, P fimbriae are not required for UTIs, because significant numbers of isolates from asymptomatic bacteriuria, cystitis, and even pyelonephritis do not express P fimbriae (1).

A considerable body of evidence from in vitro and animal studies indicates a role for type 1 fimbriae in the virulence of E. coli in the urinary tract (6-12). Type 1 fimbriae, the most common adhesive organelles of E. coli, are the prototypical examples of adhesins containing lectins, their adhesive function being inhibited by D-mannose and its derivatives (3, 13-15). Despite relatively abundant evidence, significant controversy exists concerning the role of type 1 fimbriae as a virulence factor because up to 95% of all E. coli isolates, irrespective of origin, express type 1 fimbriae, and epidemiological studies do not show differential distributions of type 1 fimbriated E. coli between uropathogenic isolates and fecal isolates of healthy individuals (1, 2, 5, 16).

Type 1 fimbriae are encoded by the fim gene cluster (17) and are composed primarily of the structural subunit, FimA. Small amounts of the adhesin subunit, FimH (18), are found at intervals along the fimbrial shaft (19) and also at the tips (20). It was recently demonstrated that type 1 fimbriae exhibit a remarkable phenotypic variation not previously appreciated (21-23). Allelic variants of the fimH gene confer distinctly different receptor specificities not limited to oligomannose structures previously thought to be the primary receptor (22, 23). Adhesion of wild and recombinant strains to three model substrata revealed at least three phenotypic classes of FimH. The M phenotype mediates adhesion only to substrates rich in exposed mannose residues, such as yeast mannan (MN). The MF phenotype mediates adhesion not only to MN, but also to complex-type oligosaccharides, such as in human plasma fibronectin. The MFP phenotype mediates adhesion to MN and fibronectin and also to synthetic peptides completely devoid of saccharide moieties (22). This functional diversity was not considered in the previous epidemiological studies.

Further studies of the predominant M phenotype surprisingly revealed that adhesion to MN could vary by up to 10-fold or more among E. coli isolates, even though the morphologies of their fimbriae were indistinguishable (23). In fact, no effective substratum was found in this previous study for the low MN-adhesive FimH phenotype predominant among fecal strains. The potential relevance of the variations in the magnitude of MN adhesion to UTIs was highlighted by the finding that type 1 fimbriated E. coli isolates obtained from UTIs exhibited an average of a 3-fold greater ability to adhere to immobilized MN than type 1 fimbriated E. coli isolated from the feces of healthy individuals (23). Ninety percent of UTI strains adhered at levels above the median level of adhesion for fecal strains (23).

The present study was undertaken to characterize the underlying molecular basis for the apparent difference in the magnitude of adhesion to MN between urinary and fecal isolates. We will show that a recombinant strain expressing FimH derived from a urinary isolate and exhibiting 10-fold greater MN adhesion than an isogenic strain expressing FimH from a fecal isolate recognizes a distinct type of oligomannoside. This difference in receptor specificity could provide a basis for differential abilities of certain strains of E. coli to target uroepithelial cells and, thereby, explain the predominance of the high MN-adhesive phenotype among UTI strains.


EXPERIMENTAL PROCEDURES

Reagents

Salivary mucin was purified from whole human saliva, as described previously (24). Monomannosylated BSA (ManBSA) was obtained from EY Laboratories, Inc. (San Mateo, CA). alpha 1-3, alpha 1-6-D-mannotriose-BSA ((Man)3BSA) was obtained from V-Labs, Inc. (Covington, LA). Human laminin was purchased from Life Technologies, Inc. Purified Tamm-Horsfall protein was generously provided by Dr. I. Ofek (Tel-Aviv University, Tel-Aviv, Israel). All other reagents were obtained from Sigma.

Bacterial Strains and Plasmids

Most of the recombinant strains utilized here were constructed using a Delta fim K-12 derivative constructed by Blomfield et al. (25) and were described previously (23). Briefly, the fim gene cluster was deleted from E. coli MG1655 to create AAEC191A (25). AAEC191A was transformed with the recombinant plasmid pPKL114 to create strain KB18. Plasmid pPKL114 is a pBR322 derivative containing the entire fim gene cluster from the E. coli K-12 strain, PC31, but with a translational stop-linker inserted into the unique KpnI site of the fimH gene. Strain KB18 cells express no fimbriae or very few numbers of long, nonadhesive fimbriae. For the studies reported here, strain KB18 was co-transformed with a series of isogenic pGB2-24-based plasmids. Plasmid pGB2-24 is a previously constructed pACYC184 derivative that is convenient for expression of fimH genes polymerase chain reaction-cloned from different E. coli (22). Recombinant strains created using these plasmids express large numbers of fully functional, type 1 fimbriae (22, 23). In most of the experiments, we employed a recombinant strain, KB91 (KB18(pGB17)), expressing the fimHF-18 gene and an isogenic strain, KB54 (KB18(pGB6)), expressing the fimHCI12 gene. E. coli F-18 is a normal intestinal isolate (26), and E. coli CI12 is a UTI isolate (22, 23). Five other isogenic recombinant strains (KB21, KB23, KB59, KB92, and KB96) differed from KB91 and KB54 only in the allelic variant of the fimH gene that was present on the pGB2-24-based plasmid. The abilities of these recombinant strains to adhere to MN were variable and corresponded to the wild strain phenotypes. The phenotypes and deduced amino acid sequences of each of these FimH subunits were described previously (23).

Another set of strains was based on a FimH- derivative of E. coli F-18 (27; gift of Dr. P. Cohen). E. coli F-18 FimH- was transformed with plasmid pGB6 (harboring fimHCI12) to create strain KBF109 or with plasmid pGB17 (harboring fimHF-18) to create strain KBF110.

Adhesion Assays

Assays of bacterial adhesion to glycoproteins bound to 96-well plates or to epithelial cells in 8-well tissue culture chamber slides (Nunc, Naperville, IL) were carried out as described previously (22, 23, 28). Briefly, glycoproteins were dissolved at 20 µg/ml in 0.02 M bicarbonate buffer, and 100-µl aliquots were incubated in microtiter wells for 1 h at 37 °C. The wells were then washed three times with PBS and quenched with 0.1% BSA in PBS. Bacteria were added in 0.1% BSA in PBS and incubated for 40 min at 37 °C without shaking to achieve saturation, and the wells were then washed with PBS. The number of bound bacteria was determined by a growth assay (28) or by using [3H]thymidine-labeled bacteria, as described previously (23). The density of bacteria used in all assays was 5 × 107 colony-forming units per 100 µl except for the equilibrium binding experiments where 12 to 16 serial dilutions of bacteria covering the densities 2.3 × 105 to 8 × 108 colony-forming units per 100 µl were utilized. Equilibrium measurements and other comparative studies were performed in parallel experiments. Adhesion to epithelial cells was determined as described previously, enumerating bound bacteria by light microscopic examination of stained samples (23).

Electron Microscopy

Suspensions of bacteria in PBS were adsorbed to Formvar-coated grids for 2 min, followed by staining on drops of 0.5% phosphotungstic acid (pH 4) for 2 min. After drying, bacteria were examined using a JEOL1200EX electron microscope.

Statistics

Correlation coefficient, r, was calculated using Cricket Graph (Cricket Software, Philadelphia, PA), where applicable. The significance of r was determined according to Fisher.


RESULTS

Adhesion to MN Correlates Directly with Adhesion to Uroepithelial Cells

We found a highly significant, direct correlation between the magnitude of adhesion to MN and the level of adhesion to either J82 human bladder epithelial cells (Fig. 1; r = 0.97, p > 0.995) or A498 human kidney epithelial cells (r = 0.93, p > 0.995; data not presented in figure) using 7 isogenic recombinant strains expressing subunits encoded by fimH genes derived from various E. coli strains. The results suggest that the differences in the magnitude of adhesion among the FimH-expressing recombinants are not restricted to immobilized MN and thus may represent fundamental differences in the fine sugar specificity of the allelic variants of FimH. To examine this possibility, the adhesion of two strains, KB91 and KB54, was studied in more detail. Strain KB91 represents the low MN-adhesive phenotype typical of normal intestinal isolates, whereas strain KB54 represents the high MN-adhesive phenotype typical of UTI isolates (23).


Fig. 1. Correlation of the abilities of seven recombinant strains to bind to MN with their abilities to adhere to J82 human bladder epithelial cells. Strain numbers are shown. Statistical analyses of the data are provided in the text.
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Scatchard Plot Analyses of Adhesion to MN

The adhesion of strains KB91 and KB54 to MN-coated wells was analyzed by equilibrium measurements. Scatchard plot analyses showed that at saturation, KB54 could utilize a maximum of 22.5 × 106 combining sites per well with a Ka ~5.0 × 10-5 and 4.4 × 106 combining sites with a Ka ~6.1 × 10-6 (Fig. 2). The analyses also revealed that the FimH of KB91 mediated adhesion through two apparent combining sites. There were approximately 4.1 × 106 low affinity combining sites per well for KB91, with a Ka ~7.1 × 10-5. Adhesion to one type of site exhibited a relatively high affinity (Ka ~1.1 × 10-7), but the number of such sites was limited to approximately 1.0 × 106 per well.


Fig. 2. Scatchard plot analyses of binding of strains KB54 and KB91 to MN at equilibrium. Data from a single experiment are presented, but the experiment was repeated several times, and the results were essentially the same. Calculations of the average association constants (Ka) and numbers of binding sites are given in the text.
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Adhesion to a Spectrum of Glycoproteins

Detection of a relatively high affinity site for strain KB91 on the MN substratum prompted us to compare the patterns of the adhesion of strains KB91 and KB54 to a variety of immobilized glycoproteins (Fig. 3). As expected, neither of these M phenotype strains bound to the glycoproteins exhibiting exclusively complex type N-linked glycans that have no terminally exposed mannosyl residues or O-linked glycans that have no mannose: human serum apotransferrin, human alpha -acid glycoprotein, and bovine milk casein. Strain KB54 adhered in large numbers to each of the other glycoproteins that are known to possess at least a certain fraction of either hybrid or high mannose type oligosaccharide moieties, both of which have terminal mannose residues. In contrast, the adhesion of strain KB91 to these substrata varied dramatically. Adhesion to one group of glycoproteins (Tamm-Horsfall protein, human amylase, salivary mucin, intestinal mucin, and mouse IgAlambda ) exhibited a clear MN-like pattern, in that KB91 adhered at a much lower level than did KB54. Adhesion of KB91 to a second group of glycoproteins (porcine thyroglobulin, chicken egg albumin, human laminin, horseradish peroxidase, and mouse IgAkappa ) was increased, but still much below adhesion of KB54. Adhesion of strain KB91 to a third group of glycoproteins (bovine lactoferrin, human secretory IgA, and bovine RNase B) was roughly equal to that of KB54, in distinct contrast to the MN-like pattern.


Fig. 3. Adhesion of strains KB54 and KB91 to various glycoproteins. Abbreviations are as follows: bovine milk casein (CS), human serum apotransferrin (TR), human alpha -acid glycoprotein (alpha aGP), yeast mannan (MN), mouse IgAlambda (mIgAlambda ), intestinal mucin (iMC), salivary mucin (sMC), Tamm-Horsfall protein (THP), human laminin (LM), chicken egg albumin (OVA), mouse IgAkappa (mIgAkappa ), horseradish peroxidase (POX), porcine thyroglobulin (TG), human IgA (hIgA), bovine lactoferrin (LF), bovine RNase B (bRB). Values are means ± S.E. (n = 3).
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Scatchard Plot Analyses of Adhesion to RNase B

Because of the high level of binding of strain KB91 and because the structure of its high mannose type oligosaccharide moiety is simpler, more well-defined, and more homogeneous than those of the other glycoproteins tested (29), bovine RNase B (bRB) was used as a model substratum in equilibrium binding experiments to compare with MN. The numbers and affinities of the combining sites utilized by the two strains were similar, with approximately 15 × 106 sites per well and a Ka ~5 × 10-6 (Fig. 4). Because equilibrium measurements showed that the parameters of adhesion of strains KB91 and KB54 to MN differed while parameters of adhesion to bRB were similar, it would appear that different mechanisms of ligand-receptor interaction were involved.


Fig. 4. Scatchard plot analyses of binding of strains KB54 and KB91 to bRB at equilibrium. Data from a single experiment are presented, but the experiment was repeated several times, and the results were essentially the same. Calculations of the average association constants (Ka) and numbers of binding sites are given in the text.
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Inhibition of Adhesion to MN and bRB by Mannosides

Although the binding of both KB91 and KB54 recombinant strains to both receptors was mannose-sensitive, the concentrations of methyl alpha -D-mannopyranoside (alpha MM) required to inhibit adhesion by 50% (i.e. the IC50) differed dramatically. The IC50 for binding of strain KB54 to bRB was 45-fold higher than the IC50 for its adhesion to MN (Fig. 5). Interestingly, the adhesion of strain KB91 to bRB was 2.5-fold less sensitive to inhibition than was the adhesion of KB54. Due to the low level of adhesion of strain KB91 to MN, the measurement of an IC50 for alpha MM was not reliable. When the abilities of D-mannose and three aromatic alpha -glycosides of mannose (i.e. octyl-, phenyl-, and nitrophenyl-mannopyranoside) to inhibit adhesion of strains KB91 and KB54 were compared, a pattern comparable with that with alpha MM was found: the adhesion of KB54 to bRB was dramatically less sensitive to inhibition than was adhesion to MN, and the adhesion of strain KB91 to bRB was significantly less sensitive to inhibition than was the binding of strain KB54 (data not shown).


Fig. 5. Inhibition of adhesion of strains KB54 (circles) and KB91 (squares) to MN (open symbols) and bRB (filled symbols) by alpha MM. Data obtained were converted to percent inhibition using the number of bacteria binding in the absence of inhibitor as control. The dotted line indicates the IC50. The x axis is on a log scale, and concentrations of alpha MM are given in mM.
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Adhesion of Recombinant Strains to Simple Mannosides Coupled to BSA

Further study of FimH binding mechanisms was performed utilizing monomannoside and trimannoside coupled to BSA (ManBSA and (Man)3BSA, respectively) as receptor substrata (Fig. 6). These correlative studies were performed using KB54, KB91, and the five other recombinant strains used above. KB54 bound to immobilized ManBSA in 12-fold greater numbers than did strain KB91, and a strong positive correlation was found between the ability of all seven recombinant strains to bind to ManBSA and their ability to bind to MN (Fig. 6A; r = 0.98, p > 0.995). All of the strains adhered relatively well to the (Man)3BSA substratum, and there was a strong positive correlation between their abilities to bind to (Man)3BSA and to bRB (Fig. 6B; r = 0.77, p > 0.95). There was no positive correlation between either the ability of the strains to bind to (Man)3BSA and MN (r = 0.0) or between the ability of strains to bind to ManBSA and bRB (r = -0.3).


Fig. 6. Correlation of the levels of adhesion of seven recombinant strains to ManBSA with their adhesion to MN (A) and correlation of the adhesion of the same strains to (Man)3BSA with their adhesion to bRB (B). Strain numbers are shown. Analyses of the data are provided in the text.
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Interestingly, while the levels of adhesion of strain KB54 to ManBSA and (Man)3BSA substrata were quantitatively similar, the alpha MM IC50 for the adhesion to ManBSA was approximately 50-fold less than the alpha MM IC50 for adhesion to (Man)3BSA, similar to the differential inhibition observed for adhesion to MN and bRB (see Fig. 5). Binding of strain KB91 to (Man)3BSA was approximately 2-fold less sensitive to inhibition by alpha MM than was the adhesion of KB54. Thus, regarding the levels of both adhesion and sensitivity to alpha MM inhibition, the reactions of E. coli with MN and ManBSA were similar, and the reactions of E. coli with bRB and (Man)3BSA were similar. These observations are consistent with the foregoing results suggesting that high MN-adhesive subunits, but not low MN-adhesive subunits, are able to mediate adhesion effectively via individual mannose residues terminally exposed in high mannose-type or hybrid-type oligosaccharide structures. At the same time, all FimH subunits are capable of mediating strong adhesion via interaction with unsubstituted trimannosyl groups.

Adhesion of Recombinant Strains Constructed in an F-18 Background to MN, bRB, ManBSA, and (Man)3BSA

Our observations in previous publications have indicated that the MS-adhesive phenotype of the two-plasmid recombinant strains used here corresponded to the MS-adhesive phenotype of wild strains and is dependent on the fimH allele (23). To determine whether the differential pattern of binding via terminal mono- and trimannoside structures described above would be seen in the MS phenotypes of wild strains, we tested the adhesion of F-18 and CI12, the fimH genes of which were used to construct E. coli KB91 and KB54, to MN, bRB, ManBSA, and (Man)3BSA (Fig. 7). The differential binding pattern observed for wild-type strains corresponded to that seen with the recombinant strains.


Fig. 7. Adhesion of wild E. coli strains CI12-7 and F-18 (A) and the recombinant E. coli strains F-18 (FimH-), KBF109, and KBF110 to bRB (square ), MN (), (Man)3BSA (), and ManBSA () (B). The patterns of adhesion of recombinant strains to these glycoproteins is dependent upon the fimHCI12 and fimHF-18 genes expressed on plasmids pGB6 and pGB17 and is basically the same as that of the wild strains. Values are means ± S.E. (n = 3).
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To determine whether the MS-adhesive phenotype of the wild strains is also determined by the fimH allele, we tested the binding of a FimH- derivative of E. coli F-18. This strain did not bind to any of the tested substrates (Fig. 7). However, when this strain was transformed with plasmids containing fimHF-18 or fimHCI12 genes, creating KBF110 and KBF109, respectively, the transformants adhered in the same pattern as did the corresponding wild strains (Fig. 7). These observations reinforce the concept that MS-adhesive phenotype is dependent primarily on the fimH allele and not the host background strain.

Electron microscopic examination demonstrated that the FimH- derivative of F-18 expressed few numbers of fimbriae per cell, essentially the same as the K-12 derivative, KB18. The fimH transformants KBF109 and KBF110, however, expressed large numbers of fimbriae typical of type 1-fimbriated wild strains and with essentially identical morphology (Fig. 8).


Fig. 8. Electron microscopy of E. coli strains F-18 (FimH-), KBF109, and KBF110. The nonadhesive strain F-18 (FimH-) expresses few fimbriae per cell. Strains KBF109 and KBF110 express large numbers of fimbriae, and the morphologies are essentially the same. × 14,400.
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Adhesion of Recombinant Strains to Uroepithelial Cells

To determine whether adhesion to human uroepithelial cells corresponds more closely to the monomannoside-type binding (MN- and ManBSA-like) or the trimannoside-type binding (bRB- and (Man)3BSA-like), a quantitative comparison was performed using all seven recombinant strains. There was a significant correlation (r = 0.98, p > 0.995) between the level of bacterial adhesion to J82 human bladder epithelial cells and the ability to bind to monomannosyl receptors (Fig. 9A). Results were similar when adhesion to A498 human kidney epithelial cells was tested (r = 1.0, p > 0.995). In contrast, there was no correlation (r = 0.05-0.08) between the adhesion of the recombinant strains to epithelial cells and their ability to bind to trimannosyl receptor structures (Fig. 9B).


Fig. 9. Correlation of the levels of adhesion of seven recombinant strains to ManBSA (A) or (Man)3BSA (B) with the level of their adhesion to J82 human bladder epithelial cells. Strain numbers are shown. Analyses of the data are provided in the text.
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DISCUSSION

Although data can be found in the literature that point toward the phenotypic diversity of type 1 fimbriae (e.g. Refs. 30-32), very little, if any, attention was previously given to this phenomenon until recently (21-23). In a previous publication, we reported that different alleles of the lectin-like subunit, FimH, mediate very different levels of adhesion of type 1 fimbriated strains to MN. We showed previously that the 10-fold differences in adhesion that were observed were not explained by different levels of incorporation of different FimH proteins into the fimbrial structure, but by differences in the FimH structure. Interestingly, the low MN-adhesive phenotype predominated among fecal strains. It was not clear why such a large fraction of the population of normal E. coli should express FimH subunits that appeared to be relatively ineffective adhesins. Here, we identify an effective substratum for the low MN-adhesive phenotype and provide a possible mechanism to explain the quantitative differences that were observed previously.

Based on the data presented here and discussed in more detail below, it can be proposed: 1) that allelic variants of the FimH lectin of E. coli type 1 fimbriae are not all alike in their ability to recognize terminal mannose structures and exhibit at least two distinct mechanisms of ligand-receptor interaction; 2) that all FimH subunits studied to date are capable of mediating adhesion via trimannosyl residues, but only certain variants are capable of mediating adhesion via monomannosyl residues; and 3) that the ability of the FimH lectins to interact with monomannosyl residues strongly correlates with their ability to mediate E. coli adhesion to uroepithelial cells. Whether these phenotypic differences result in differences in tissue tropism in a human or animal host remains to be determined.

The hypothesis that different receptor specificities are responsible for the apparent magnitude of adhesion to MN was prompted by equilibrium binding measurements of adhesion of strains KB91 and KB54 to MN. Scatchard plot analyses of bacterial adhesion data can provide important information regarding receptor specificity, giving an indication of both affinity and the heterogeneity of binding sites (33). Scatchard plot analyses indicated the possibility that strain KB91 reacts relatively weakly with the high affinity MN receptors recognized by strain KB54 and either does not react or reacts at undetectable levels with the low affinity MN sites of strain KB54. However, a number of high affinity binding sites for strain KB91 in MN were detected, indicating the ability of this FimH adhesin to interact strongly with certain receptor structures which were exposed poorly in immobilized MN. Indeed, while KB91 exhibited low levels of adhesion to MN, it adhered avidly to several glycoprotein substrata, with IgA, lactoferrin, and bRB being the most prominent of those tested thus far. The equilibrium analyses of adhesion to bRB indicated that the FimH lectins of both strains probably interact with the same structural element on the bRB-coated surface and with the same effectiveness. Thus, it is likely that separate mechanisms of ligand-receptor interactions are responsible for the differential adhesion of these two strains to MN and bRB.

Inhibition of ligand-receptor interactions by receptor analogs is an important adjunct to direct adhesion studies. The more effective inhibition of bacterial adhesion to MN by soluble monomannosides than adhesion to bRB prompts us to suggest that the mechanism of adhesion to MN is primarily via interaction with single terminal mannose residues, whereas adhesion to bRB involves a more complex interaction with multiple mannose residues. Indeed, strain KB54 was able to adhere much better than strain KB91 to ManBSA, whereas both strains adhered well to (Man)3BSA. Interestingly, although the N-linked carbohydrate moieties of both MN and bRB do provide terminal mannosyl residues, terminal mannotriose structures are abundant in the Man5 and Man6 oligomannose units which constitute almost 90% of bRB oligosaccharide units, but not in the mannoproteins of Saccharomyces cerevisiae (34). Also, it is known that human IgAs contain hybrid-type oligosaccharides which have terminal mannotriose structures (35), whereas ovalbumin contains a mixture of oligomannose, hybrid, and multiantennary complex N-linked glycans. Whether other oligomannose structures would provide increased or decreased levels of adhesion in comparison to the trimannoside remains to be determined.

It has been proposed previously that the combining site of the type 1 fimbrial lectin is in the form of a complex, trisaccharide-sized pocket that has three adjacent subsites, each of which accommodates one residue of the trisaccharide (36). Many previous studies of the fine sugar specificity of type 1 fimbriae called attention to oligomannose structures as the primary, if not exclusive, receptors. However, the precise nature of the ligand-receptor interactions was not fully developed (14, 15). It is now reasonable to speculate that the strong binding of KB91 and KB54 FimH subunits to trimannosyl structures occurs via the interaction of the subsites of the combining pockets of both adhesins with multiple mannosyl residues. The ability of KB54 subunits to mediate adhesion to monomannosides could be due to the ability of a single subsite to react with sufficient affinity to accomplish adhesion. The requirement of the KB91 FimH for trimannose units could be because none of the subsites has a structure that allows high affinity interaction with a single mannosyl residue. The hypothesis that FimH can mediate adhesion via binding to monomannoside residues is quite novel for type 1 fimbriae and could have significant physiological implications.

Neither MN nor bRB are likely to be important receptors for E. coli on host mucosal surfaces. However, their use as model substrata helped to identify two mechanisms of interaction of the FimH lectins which could help to dissect the mechanism of binding to physiologically relevant glycoproteins (e.g. salivary and intestinal mucins (37), IgA (35), lactoferrin (38), uroplakins (39), leukocyte integrins (40), etc.). The divergent mechanisms of interaction of FimH with terminal mannosyl residues described here imply that FimH subunits recognizing monomannoside residues, as represented by the KB54 FimH, should mediate a broader spectrum of bacterial adhesion than FimH subunits recognizing only trimannoside residues, as represented by the KB91 FimH. The stricter selectivity of the trimannose-specific FimH subunits may allow targeting of the strains to surfaces that are physiologically important for normal intestinal E. coli and may help to avoid or reduce the impact of nonspecific host defense barriers, such as lectino-phagocytosis (41). The reduced sensitivity of the trimannose-specific FimH subunits to the inhibition by soluble compounds containing exposed mannose could be another advantage of this phenotype. Thus, the trimannose-specific phenotype could provide more efficient adhesion for E. coli cells in an environment where mucosal surfaces are bathed with mannose-rich glycoproteins. On the other hand, strains bearing the monomannose-specific FimH subunits may have an increased chance to find a substratum containing an acceptable mannosylated compound. Such an expanded receptor specificity for the monomannose-specific FimH might provide a selective advantage for type 1-fimbriated E. coli in the colonization of certain ecological niches (42) and, for example, may be of great importance in the contribution these organelles make to the virulence of E. coli in the urinary tract (12). Although the exact structure of the oligosaccharides exposed on the uroepithelial surface is not yet defined (38), the strong correlation between the ability to bind to ManBSA and the ability to bind to uroepithelial cells among the FimH-expressing strains suggests that bacterial attachment is accomplished primarily via the monomannoside binding mechanism. Thus, the enhanced ability to bind to uroepithelial cells may explain why UTI isolates express predominantly fimbriae bearing monomannose-specific FimH.

It is not yet clear precisely how uropathogenic E. coli orchestrate the contributions of type 1 fimbriae and other virulence factors in the complex process that results in cystitis or pyelonephritis. The observations reported here strongly suggest that some phenotypic variants of FimH contribute much more to UTI than do others by increasing the ability of E. coli to adhere better to uroepithelial cells. It may be of interest in this regard to consider the recent observations reported by Connell et al. (12). Clonally related uropathogenic strains of E. coli O1:K1:H7 were tested for virulence in a mouse UTI model. The strains expressed type 1 and P fimbriae and were shown to be members of the same clone by serotyping and by multilocus enzyme electrophoresis. However, they were recovered in dramatically different numbers from kidneys and bladders after experimental UTI in mice. We have recently found that the more highly virulent strain recovered from kidneys and bladders in high numbers expresses monomannose-specific FimH, while the strain exhibiting relatively low virulence and recovered from kidneys and bladders in much lower numbers expressed trimannose-specific FimH.2


FOOTNOTES

*   This work was supported by Veterans Affairs Medical Research funds, by United States Public Health Service Grant DE-07218 (to D. L. H.), by the United States-Mexican Science Foundation (to R. J. D.), and by a grant from the Kentucky branch of the American Lung Association (to R. J.  D.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
§   Current address: Dept. of Microbiology, Nations Friendship University, Moscow, Russia.
**   To whom correspondence should be addressed: Research Service (151), Veterans Affairs Medical Center, 1030 Jefferson Ave., Memphis, TN 38104. Tel.: 901-523-8990 (Ext. 7556); Fax: 901-577-7273; E-mail: dhasty{at}utmem1.utmem.edu.
1   The abbreviations used are: UTI, urinary tract infection; MN, yeast mannan; ManBSA, mannosylated bovine serum albumin; (Man)3BSA, trimannosyl-BSA; alpha MM, methyl-alpha -D-mannopyranoside; bRB, bovine RNase B; PBS, phosphate-buffered saline.
2   E. V. Sokurenko and D. L. Hasty, unpublished observations.

ACKNOWLEDGEMENTS

We thank Drs. Ithzak Ofek, Harry Courtney, and James Dale for stimulating discussions and for helpful comments during the preparation of the manuscript.


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