Antisera Directed against Anti-Histone H4 Antibodies Recognize Linker Histones
NOVEL IMMUNOLOGICAL PROBES TO DETECT HISTONE INTERACTIONS*

(Received for publication, November 18, 1996, and in revised form, May 22, 1997)

Christophe Thiriet and Jeffrey J. Hayes Dagger

From the Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York 14642

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES


ABSTRACT

We introduce a novel immunological approach to detect structural interactions between chromosomal proteins. Antigenically pure core histone H4 was prepared from chicken erythrocytes and used to produce anti-histone H4 antisera. IgG fractions were isolated from purified anti-H4 antibodies and used as antigens to produce "second generation" antisera. Epitopes cross-reacting with the second generation antisera were then identified within chromosomal proteins. These epitopes were presumed to mimic the complementary molecular surface of the original anti-H4 antibodies, and thus proteins containing these epitopes were putatively identified as specific ligands of H4 in chromatin. Surprisingly, we found this immunoreactivity was predominantly directed against H1 compared with H5 from chicken erythrocytes. Further, the immunoreactive epitopes were located within the C-terminal tail domain of the linker histones. These results suggest similar complementary interactions occur between H4 and the C-terminal tail domain of H1s in native chromatin. This could occur either within a single nucleosome as suggested by a previous report (Banères, J.-L., Essalouh, L., Jariel-Encontre, I., Mesnier, D., Garrod, S., and Parello, J. (1994) J. Mol. Biol., 243, 48-59) or between neighboring nucleosomes within the condensed chromatin fiber. The implications of these results with regard to the structure of the chromatin fiber and the future utility of this technique are discussed.


INTRODUCTION

The primary repeating subunit of chromatin is a protein-DNA complex composed of a histone protein octamer, (H2A/H2B/H3/H4)2, a single linker histone protein, and approximately 200 base pairs of DNA (1, 2). Adjacent nucleosomes are connected together by a DNA linker of variable length depending on the species (3). Nucleosomal structures are generally considered to have a fundamental role in many nuclear activities which involve DNA, such as transcription, replication, recombination, and DNA repair (2). Modifications of histone proteins may modulate the condensation/decondensation of the chromatin fiber, which in turn changes the accessibility of the DNA to enzymes or factors involved in these nuclear activities (2, 4-8).

The histone octamer is comprised of four heterotypic dimers comprised of either H2A and H2B or H3 and H4. The majority of each histone protein is contained within an evolutionarily conserved structure know as the "histone fold," which is comprised of a long, central alpha -helix flanked on each side by a short loop and a short alpha -helix (9-11). Two histone fold proteins interlock via the "handshake" motif with extensive complementary surfaces between heterodimerization partners (10). Histone dimers associate in an end-to-end fashion to form essentially a H2A/H2B-H4/H3-H3/H4-H2B/H2A helical tetramer of dimers onto which the DNA is wrapped (9, 10, 12).

Despite the well characterized nature of intra-core histone interactions, other histone-histone interactions likely to be found in chromatin have not been as well described. For example, in the compacted chromatin fiber it is likely that core histone proteins from nearby nucleosomes are brought into close proximity, and it has been proposed that inter-nucleosomal core or even histone-histone interactions are instrumental in directing the condensation of the fiber (13, 14). One manifestation of these interactions may be the propensity with which core histone octamers "close-pack" together when reconstituted with long DNA in vitro in the absence of linker histones (15). In addition, the interactions of many non-histone chromosomal proteins such as HMGs 1/2 or 14/17, which are likely to interact with multiple proteins within chromatin (16), have not been well defined.

Immunological approaches for analyzing the structural relationships between chromatin proteins have been extremely useful in the investigation of protein structures within chromatin (17). Indeed, since Stollar and Ward (18) first demonstrated that histones are adequate immunogens when complexed with RNA, numerous laboratories have obtained anti-histone antisera. The use of such antisera and anti-histone antibodies as probes has allowed the detection of many details of the surface of chromatin subunits, despite the limited amount of information concerning the molecular structure of epitopes found at the surface of these structures (19, 20). Further, Muller and colleagues have demonstrated that the production of antisera to synthetic peptides that mimic linear histone regions (21) could be used to detect the histone regions accessible to anti-peptide antibodies inside chromatin (4, 19-21). Anti-histone immunochemical reagents also have been instrumental in the elucidation of the role of histone posttranslational modifications in nuclear processes such as transcription and replication (6, 22, 23).

In the present report, we present a novel immunological approach to detect potential protein-protein interactions within chromatin. This approach is based on the complementarity of surfaces that are in contact within interacting systems. This phenomenon is exemplified by hormone/hormone receptor systems in which it has been demonstrated that antibodies to anti-hormone antibodies are able to mimic physiological effects of hormones (reviewed in Ref. 24; see "Discussion"). This effect is due to the complementary structures of the receptor and ligand within these systems and the fact that anti-anti-hormone antibodies recapitulate many of the key facets of the original hormone structure (see Fig. 1). Here we demonstrate that IgG fractions from anti-H4 antibodies are able to induce production of antibodies that react with the original IgG fractions and also demonstrate specific immunoreactivity against the C-terminal tail domain of linker histones.


Fig. 1. Scheme of the method to detect proteins which make complementary interactions with histone H4. Anti-H4 antibodies are used to raise anti-anti H4 antibodies which are in turn used to identify H4-complementary proteins. The black and stippled double-headed arrows indicate complementary immunological and native protein-protein interactions, respectively.
[View Larger Version of this Image (15K GIF file)]


MATERIALS AND METHODS

Preparation of Histone H4

Chicken erythrocyte nuclei were isolated according to the procedure of Nothacker and Hildebrandt (25) with the modifications described by Loidl and Gröbner (26). Histones were extracted from nuclei with H2SO4 as described by Helliger et al. (27). Histone H4 was purified by chromatography on a Bio-Gel P-60 (Bio-Rad) column (28), followed by a series of chromatographic fractionations on Sephadex G-100 column (Pharmacia Biotech Inc.) equilibrated in 50 mM sodium acetate successively at pH 5 and 4 as reported by Muller and Van Regenmortel (29). Rabbit antiserum immunoreactive against all histone proteins (RAAHC)1 was prepared using acid extractable proteins from chicken erythrocyte nuclei. The purity of histone H4 was then assessed by Western blot probed the RAAHC antiserum.

Preparation of Antisera

Antisera against purified histone H4 of chicken erythrocyte were obtained by immunizing mice biweekly with H4·RNA complexes (3:1, w/w) as previously reported by Stollar and Ward (18). For each vaccination, each animal received by subcutaneous injection 10 µg of histone H4 emulsified in complete Freund's adjuvant for the first injection or in incomplete Freund's adjuvant for the subsequent injections. Antisera against IgG fractions either from anti-H4 antibodies or from antibodies recognizing no histone (control) were obtained by immunizing mice biweekly by subcutaneous injections with 5 µg of IgG molecules emulsified in complete Freund's adjuvant for the first injection or in incomplete Freund's adjuvant for subsequent injections. Antiserum was collected at regular intervals over a period of 20 weeks.

Preparation of Immunoglobulin G Fractions

Specific antibodies against H4 were isolated from anti-H4 antiserum by immunoaffinity on a nitrocellulose sheet according to the procedure described by Olmsted (30), with minor modifications. Briefly, chicken erythrocyte histones are resolved by preparative SDS-PAGE, electroblotted onto nitrocellulose sheet (31). The edge of the membrane was stained with Amido Black to localize histone H4 on the nitrocellulose sheet. After cutting out the band corresponding to H4, the nitrocellulose strip is saturated with 1% ovalbumin diluted in PBS-T (phosphate buffered saline with 0.05% Tween 20). Then the strip was incubated with anti-H4 antiserum diluted 1:10 in PBS-T for 1 h under gentle agitation. Unbound components from the antiserum were removed by washing the nitrocellulose strip three times in PBS-T for 5 min each with gentle agitation. Specific H4-antibody complexes were then eluted by washing the strip in 0.1 M glycine-HCl, pH 2.5, three times for 5 min each at room temperature with gentle agitation. The three acid washes were combined, the pH of the solution was adjusted to 7 by addition of 1 M Tris-HCl, pH 9, and the solution was dialyzed overnight against PBS. Control serum was prepared from mice immunized with mouse IgG molecules depleted of any trace anti-histone immunoreactivity by exhaustive immunoaffinity on nitrocellulose sheets onto which all histone proteins had been adsorbed. This procedure was repeated three times, and then fractions were dialyzed overnight against PBS. Control and anti-H4 IgG fractions were then isolated by affinity chromatography on Sepharose CL-4B covalently linked with Staphylococcus aureus protein A (Pharmacia). Protein A-antibody complexes were separated as described above, the IgG fractions were finally dialyzed overnight against PBS, and the absence of detectable amounts of histones was confirmed by Western blot.

Immunoblotting

Protein samples were resolved by SDS-PAGE (32) and then electroblotted onto nitrocellulose sheets (31). The nitrocellulose sheets were blocked in (PBS-T) and 1% ovalbumin for 30 min at room temperature. The filters were then incubated with diluted primary antiserum for 1 h, washed three times for 5 min with PBS-T, and incubated for 1 h with diluted peroxidase-conjugated anti-IgG (Jackson, Interchim, France) or with peroxidase-coupled protein A. The membranes were finally washed thoroughly in PBS-T, and peroxidase was detected as described by Harlow and Lane (33). The same procedure was followed for the Dot Blot assay. For inhibition experiments, nonimmune serum, anti-H4 antiserum, or purified histones were incubated overnight at 4 °C with antiserum directed against anti-H4 IgG fractions. After centrifugation, the supernatant was used to immunoprobe histones resolved by SDS-PAGE. The reactivity of immune response was monitored by measurement of the optical density with a gel scan analyzer (Vilber Lourmat, France).

Preparation of Linker Histones

Calf thymus and chicken erythrocyte linker histones were prepared as described previously (34). Bacterially expressed H1°a and the C-terminal deletion mutant of this protein containing residues 1-101 were prepared as described elsewhere (35). Purified Tetrahymena H1 was a gift from Dr. David Allis, University of Rochester. H1 globular domain was prepared by treating 50 µg of total acid-extracted calf thymus H1 with 100 ng of trypsin in 10 mM Tris-HCl, 1 mM EDTA, 100 mM NaCl for 45-60 min at room temperature. The reaction was terminated by the addition of an excess of soybean trypsin inhibitor (Worthington) and used directly for SDS-PAGE.


RESULTS

Production of Specific Anti-H4 Antisera

Our approach was to raise antibodies against purified anti-H4 antibodies and then to determine which chromatin proteins cross-react with this antiserum (Fig. 1). Thus, we required antigenically pure H4. To ensure the purity of our H4 preparation we first isolated this histone by chromatographic methods and detected the protein by Western blot of fractions resolved on SDS-PAGE with antiserum directed against all histone proteins (RAAHC, see "Materials and Methods"). We obtained a fraction that clearly yielded a single band corresponding to histone H4 (data not shown but see Fig. 2). We then immunized three mice with the highly purified H4 fraction and monitored the resulting immune response over an extremely long immunization period. The results obtained with one animal are shown in Fig. 2A and correspond to the intensity of the immune response 1 week after each vaccination with the antigenic mixture. We observed that the immune response remained specific to histone H4 throughout the immunization period, although insignificant responses against H3 were observed at the beginning of the series of immunizations with this animal. Moreover, from the 5th to the 10th and final vaccination, the intensity of the immune response against H4 did not increase, but the antisera remained specific to histone H4. It is clear from the Western blot presented in Fig. 2B corresponding to the analysis of antiserum obtained after the 10th immunization that this antiserum is specific only to histone H4 when it is tested against all acid-extractable nuclear proteins. Thus, we have obtained even after a long immunization period specific antisera directed against histone H4, which is considered to be the least immunogenic chromosomal protein (17). These results show that our H4 preparation is highly antigenically pure and raises an immune response only against H4 protein within the mice. The antiserum produced against this preparation of H4 was selected as a source of anti-H4 antibodies for further studies and will be referred to as MAH4 antiserum.


Fig. 2. Production of anti-H4 antibodies by immunization of a mouse with histone H4·RNA complexes. A, antibody levels as measured by Western blots. 5 µg of total histones were resolved by SDS-PAGE, blotted, and probed with antiserum diluted 1:1000 from a mouse immunized with purified histone H4. The intensity of immune response was then monitored by optical density measurement (vertical axis, arbitrary units). triangle , anti-H3 antibodies; open circle , anti-H4 antibodies. Arrows indicate the immunization schedule (10 µg of H4 and 3.3 µg of RNA complex/injection). B, immunodetection of histones with anti-H4 antiserum obtained after the 10th vaccination. The left blot shows Amido Black-stained proteins and the right blot corresponds to the immunoblot with anti-H4 antiserum diluted 1:1000.
[View Larger Version of this Image (20K GIF file)]

IgG Fractions from Anti-histone H4 Antiserum Mimic Histone Epitopes

To easily determine whether the IgG fractions from anti-H4 antiserum were able to mimic any histone epitopes, we carried out the Dot Blot shown in Fig. 3. Different amounts of IgG fractions purified from MAH4 antiserum or mouse preimmune serum were deposited on a nitrocellulose sheet. The IgG fractions were then probed with RAAHC. Note that the use of the IgG fractions from two different species was required to ensure that secondary antibodies only will detect interactions between the IgG from MAH4 antiserum (mouse) and the RAAHC antiserum (rabbit) used as primary antibodies. We observed significant immunoreactions with the IgG fractions from MAH4 but not those prepared from preimmune serum. Clearly IgG molecules from anti-histone H4 antiserum are capable of mimicking histone epitopes. As a control we also determined that the anti-H4 IgG preparation exhibited no detectable immunoreaction against the co-injected RNA (results not shown).


Fig. 3. Immunoreactivity of IgG fractions from preimmune and anti-H4 antisera against all classes of histone proteins. IgG fractions from mouse preimmune or anti-H4 sera were spotted onto a nitrocellulose filter and probed with a 1:1000 dilution of antisera raised against a preparation containing all types of histone proteins (see "Materials and Methods"). The amount of IgG protein spotted is indicated.
[View Larger Version of this Image (69K GIF file)]

Antisera against IgG Fractions from Anti-H4 Antibodies Recognize Linker Histones

We wished to determine by immunochemical means which histone proteins contained the epitopes mimicked by the anti-H4 IgG fractions. To this end, we first purified the antibody fraction directed against H4 by nitrocellulose immunoaffinity fractionation of the antiserum as described previously by Olmsted (30). The purified antibody fraction was then deposited on a protein A column, and the IgG fractions were purified from other anti-H4 antibodies. The purity of the IgG fractions was finally assessed with RAAHC antiserum. The results presented in Fig. 4 show that there is no evidence of any histone protein contaminating these purified anti-H4 IgG fractions. Interestingly, in this Western blot, we observe immunodetection of the heavy chains of the IgG fractions, even when the RAAHC antiserum was omitted (data not shown). It seems therefore that denaturation of mouse IgG molecules in SDS-polyacrylamide gel exposes common epitopes between mouse IgG and rabbit IgG molecules, which are not revealed when the IgG molecules are folded in their native state (compare the IgG from preimmune serum in Fig. 3 and the IgG lane in Fig. 4).


Fig. 4. Assessment by Western blot of the absence of histone in the IgG fractions from anti-H4 antibodies. A, peptides stained with Amido Black and B, immunoblot with antiserum directed against all acid extracted nuclear proteins. IgG and CE correspond to IgG fractions from anti-H4 antibodies (H, heavy chains; L, light chains) and to chicken erythrocyte histone proteins from acidic extraction, respectively. The positions of linker histones (LH) and core histones (core) are indicated. Detection of the heavy chain is due to the use of peroxidase-protein A to detect the immunocomplexes. Protein size standards of 66, 45, 31, 21.5, 14.4, and 6.5 kDa are shown, from top to bottom, respectively (lane M).
[View Larger Version of this Image (60K GIF file)]

The purified anti-H4 IgG fractions were then used as antigens to induce antibody production in mice. Three animals were immunized by a series of subcutaneous injections of 5 µg each of anti-H4 IgG molecules over a period of 5 months. After a series of immunizations one mouse failed to respond against histone, but the other two mice induced similar and satisfactory immune responses. As a control for any immune response against histones due to the IgG molecules themselves, several mice were immunized with mouse IgG molecules that had been exhaustively depleted of immunoreactivity against any of the histone proteins (see "Materials and Methods").

Sera from mice immunized with the purified anti-H4 IgG fractions were tested for immunoreactivity against all histone subtypes. Western blots of acid-extracted chromatin proteins were probed with antiserum from the two mice that developed antibodies against histones and from two control mice immunized with IgG fractions depleted of anti-H4 immunoreactivity. A time profile of the immunological response from the serum obtained from these mice is shown in Fig. 5. After one immunization with purified anti-H4 IgG an unusually rapid and very weak response was detected against histone H3 (Fig. 5). In contrast, after seven to eight immunizations with the purified anti-H4 IgG fractions, a much stronger and more typical response was detected against linker histones (Fig. 5). Moreover, the intensity of immune response against linker histones increased to much higher levels than the response against histone H3 (Fig. 5B). No reactivity against either linker histone or H3 is detected with antisera raised against IgG molecules that were depleted of anti-histone immunoreactivity (Fig. 5A). Furthermore, no such reactivity was observed after similar numbers of immunizations with purified H4 (Fig. 4) or with over 50 mice immunized with various histone antigens.2


Fig. 5. Immunoreactivity against histones of antiserum from mice immunized with IgG fractions from anti-H4 antibodies. Acid extracted histone proteins were separated by SDS-PAGE and Western blotted with the antiserum raised against anti-H4 IgG fractions. A, time course of immune response of mice immunized with IgG fractions from anti-histone antibodies. Shown are Western blot analysis of antisera from two mice immunized with IgG fractions from anti-H4 antibodies (I and II) and two mice immunized with IgG fractions from antibodies recognizing no histone (III and IV). 5 µg of histones resolved by SDS-PAGE were blotted and probed with antisera diluted 1:1,000. 1:100, or 1:10,000 yielded similar detections. The gel at the far left shows the Amido Black-stained gel of histone proteins used for the blots. Blots of these proteins were probed with antisera obtained, respectively, after 1, 9, and 10 immunizations are shown as indicated in the panel corresponding to each mouse. Open arrows on the right indicate the positive immunoreactivity detected against linker histones H1s and H5 (top) and histone H3 (bottom). B, plot of immune response against specific histone proteins detected in A versus time. open circle , anti-H3 antibodies; diamond , anti-H1 antibodies; triangle , anti-H5 antibodies. The intensity of the immune response was monitored by optical density measurement (vertical axis, arbitrary units). Arrows indicate the immunization schedule (5-µg IgG fractions from anti-H4 antibodies).
[View Larger Version of this Image (28K GIF file)]

To confirm that the specific immunoreactivity we detected was actually induced by the H4-complementary portions of the anti-H4 antibodies, we carried out inhibition assays. Increasing quantities of either control serum or anti-H4 antiserum (MAH4 antiserum) were added to the mouse antisera raised against the anti-H4 IgG fractions, and the effects on the immunoreactivity of the latter serum were determined. When increasing amounts of the control serum were added, there was no evidence of any inhibition of the immunoreactivity of the mouse antiserum raised against anti-H4 IgG to either immobilized linker histones (Fig. 6A) or H3 (results not shown). In contrast, increasing quantities of MAH4 antiserum clearly inhibited the binding of anti-anti-H4 IgG fractions to these immobilized proteins. Moreover, Western blots of IgG fractions purified from anti-H4 antiserum and probed with antiserum from mice immunized with these antigens showed no evidence of any specific recognition of denatured IgG molecules and the antiserum (results not shown). This result strongly suggests that only native IgG purified from anti-H4 antibodies and not the IgG molecules themselves are able to induce immune responses against histones and that antibodies raised against anti-H4 IgG fractions are directed against the idiotopes of the anti-H4 antibodies. We thus conclude from these experiments that IgG fractions from anti-H4 antibodies induced the production of antibodies that are directed against linker histones, and that this effect is dependent upon the original H4 epitopes.


Fig. 6. Specific inhibition of anti-H1 immunoreaction by the antiserum raised against anti-H4 IgG fractions by anti-H4 antiserum. Increasing quantities of either mouse normal serum (A) or anti-H4 antiserum (B) were added to a 1:1000 dilution of antiserum directed against anti-H4 IgG fractions. Mixtures were tested by Western blot with 150 ng of H1 resolved by SDS-PAGE. Percent inhibition of the specific response is plotted against microliters of serum or antiserum added with 100% taken as the uninhibited response. black-diamond , black-square, and bullet  represent the reactivity against all H1 species, H1a, and H1b, respectively.
[View Larger Version of this Image (17K GIF file)]

We wished to further isolate the epitopes responsible for the observed specific immunoreation. Because of the atypical and weak profile of reactivity detected against H3, we chose to further characterize only the immunoreactivity detected against linker histone. A blot of H1s from calf thymus, bacterially expressed Xenopus H1°, and linker histones isolated from chicken erythrocytes and Tetrahymena revealed strong reactivity against H1 subtypes (Fig. 7). Interestingly, much less relative response was detected against the H5 or H1° subtypes compared with H1s (Fig. 7; compare bands in lane 2 and lanes 1 versus 3). We also observed specific immunoreactivity against the Tetrahymena H1, a protein that does not have a protease-resistant globular domain (1, 35). This suggests that the common epitopes reside in the flexible N- or C-terminal tail domains. Indeed, we find the reactive epitopes are not present within a preparation containing only the protease-resistant globular domain of linker histones (Fig. 7, lane 5) or in a linker histone peptide lacking only the C-terminal tail domain (lane 6).


Fig. 7. Immunoreactivity of linker histones from different species with anti-anti-H4 antiserum. Gel, Coomassie staining of linker histones and globular domains of linker histones resolved in SDS-PAGE. Blot, immunodetection of peptides with anti-anti-H4 antiserum, previously resolved in SDS-PAGE and transferred onto nitrocellulose. The lanes 1-6 correspond, respectively, to calf thymus H1s, linker histones isolated from chicken erythrocyte, recombinant H1° from Xenopus, linker histones from Tetrahymena, globular domain of calf thymus linker histones obtained by trypsin treatment, and globular domain of Xenopus H1° obtained by bacterial overexpression. The schematics in the center indicate the presence or absence of the short N-terminal tail (short horizontal line), globular domain (oval), and C-terminal tail domain (long horizontal line) in each of the samples analyzed.
[View Larger Version of this Image (32K GIF file)]


DISCUSSION

The major conclusion of this study is that antibodies directed against histone H4 can serve as antigens to induce the production of antibodies directed against other histone proteins and that this method has identified specific protein-protein interactions that might occur within native chromatin (Figs. 5 and 6). We demonstrated that immunization of mice with purified IgG fractions from anti-H4 antibodies induced the production of antibodies against the antigen but, significantly, these antibodies also specifically recognized linker histones and, perhaps, to lesser extent histone H3. However, the kinetics of these two responses were quite different (Fig. 6), and it is unclear at this time why the response against H3 was so unexpectedly rapid and weak in nature, and why it did not increase with subsequent injections. Nonetheless, the response against linker histones followed a kinetic profile typical for an immunogenic reaction and yielded a much higher antibody titer. For these reasons we further characterized only the anti-linker histone immunoreactivity.

Several lines of evidence suggest that the specific response observed is most likely due to antibodies produced specifically against anti-H4 IgG. First, immunization with IgG fractions depleted of anti-H4 immunoreactivity did not give rise to antisera that exhibit immunoreactivity against any histone proteins. Second, we found the response is specific, such that either linker histones or the purified anti-H4 IgG fraction can effectively inhibit the anti-linker histone reactivity of antisera raised against anti-H4 IgG. Finally, similar specific responses were obtained with two mice against only certain histone proteins and were not obtained with control mice immunized with control IgG fractions. Interestingly, the immunoreactivity is largely directed against the H1-type histones relative to the H5 or H1° subtypes (Fig. 6). Thus, anti-idiotypic reactivity is predominantly against the least-represented subtype of linker histone found in the erythrocyte cells from which the H4 was prepared, arguing against contamination of the H4 preparation by linker histone.

Previous work has demonstrated that H1 exists in close proximity to the N-terminal tail of histone H4 within chromatin (36). We detected cross-species H1 reactivity, suggesting the putative H4-H1 interaction is a general feature of H1-containing chromatin. We have demonstrated that the simultaneous recognition of IgG fractions from anti-H4 antibodies and the histone proteins occurs via the same immunoreactive entity. Thus both the IgG fractions and linker histones possessed elements of complementary structures with the anti-anti H4 antibodies (summarized in Fig. 1). Interestingly, the immunoreactive epitopes existed within the C-terminal tail domain of H1 (Fig. 7). We conclude that the C-terminal tail domain of H1s must possess surfaces or structures that are complementary with histone H4 and that possibly the H4 N-terminal tail domain specifically interacts with linker histones in vivo.

A recent model of the binding of linker histone to the nucleosome suggests that the globular domain of this protein is situated near a point where the H4 N-terminal tail cross-links to the DNA (37, 38). This would bring the H1 C-terminal domain into close proximity to the H4 tail. Alternatively, it is possible that H4 makes contact with linker histone internucleosomally, i.e. between neighboring nucleosomes, which are made when the chromatin fiber is compacted. Experiments to determine the availability of linker histone epitopes in extended and condensed chromatin fiber are presently underway. Further characterization of the epitopes which exhibit complementary structures with H4 can be made by employing peptides from identified proteins (21). Preliminary results with the mouse antiserum raised against anti-H4 IgG used in the present work suggested that the epitopes within linker histone are located near the end of the C-terminal tail domain.2 It is interesting to note that antibodies induced by immunization with anti-histone antibodies possessed a high degree of specificity for histones. The use of monoclonal antibodies may also allow a finer determination of exactly what epitopes are complementary but the production of monoclonal antibodies against histones is somewhat problematic (39-42).

Clearly, our approach does not detect all species interacting with the substrate in question. Within the histone octamer, H4 makes some contacts with histones H2B at the dimerization interface between the H4/H4 and H2A/H2B dimers (9). There are several possibilities for absence of reactivity of anti-anti-H4 with H2B. All regions of H4 are not immunogenic (4), and it is possible that the anti-H4 IgG fraction may not contain antibodies specific for the region of H4 that interacts with H2B. It is possible that this surface within H4 may be somewhat masked within the H4·RNA complex used to raise the initial anti-H4 antisera. The conformation of H4 within the H4·RNA complex is probably very different from that when this protein is complexed with histone H3 (43). Thus, the H2B-interacting surface of H4 may be more properly presented within the (H3/H4)2 complex. Experiments to test this possibility are underway.

We have investigated whether the histones interacting with H4 could be detected by antibodies by using the strategy successfully applied to hormone/hormone receptor systems (reviewed in Ref. 24). The novel immunological probes described in the present work demonstrate that the interactions between H4 and the linker histone are probably due to the recognition of complementary motifs found at the surface of these histones. Future applications of this approach could detect other interactions between histone proteins which may be relevant to contacts made between nucleosomes within the chromatin fiber as well as shed light on the poorly defined protein-protein interactions made by the many non-histone chromosomal proteins within chromatin.


FOOTNOTES

*   The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Dagger    Supported by National Institutes of Health Grant R01GM52426. To whom correspondence should be addressed. Tel.: 716-273-4887; Fax: 716-271-2683.jjhs{at}uhura.cc.rochester.edu.
1   The abbreviations used are: RAAHC, rabbit antiserum raised against all histone proteins; MAH4, mouse antisera raised against the anti-H4; PAGE, polyacrylamide gel electrophoresis; PBS, phosphate-buffered saline.
2   C. Thiriet, unpublished results.

ACKNOWLEDGEMENTS

We thank Drs. C. Dave Allis, Martin Gorovsky, and Cheeptip Benyajati for a critical reading of the manuscript and for helpful discussions.


REFERENCES

  1. Van Holde, K. E. (1989) Chromatin, Springer-Verlag, New York
  2. Wolffe, A. P. (1995) Chromatin Structure and Function, Academic Press, London
  3. Kornberg, R. D. (1977) Annu. Rev. Biochem. 46, 931-954 [Medline] [Order article via Infotrieve]
  4. Muller, S., Chaix, M. L., Briand, J. P., and Van Regenmortel, M. H. V. (1991) Mol. Immunol. 28, 763-772 [Medline] [Order article via Infotrieve]
  5. Thoma, F., Koller, T., and Klug, A. (1979) J. Cell Biol. 83, 403-427 [Abstract]
  6. Hebbes, T. R., Thome, A. W., and Crane-Robinson, C. (1988) EMBO J. 7, 1395-1402 [Abstract]
  7. Felsenfeld, G. (1992) Nature 355, 219-224 [CrossRef][Medline] [Order article via Infotrieve]
  8. Garcia-Ramirez, M., Rocchini, C., and Ausio, J. (1995) J. Biol. Chem. 270, 17923-17928 [Abstract/Free Full Text]
  9. Arents, G., Burlingame, R. W., Wang, B. C., Love, W. E., and Moudrianakis, E. N. (1991) Proc. Natl. Acad. Sci. U. S. A. 88, 10148-10152 [Abstract]
  10. Arents, G., and Moudrianakis, E. N. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 10489-10493 [Abstract]
  11. Baxevanis, A. D., Arents, G., Moudrianakis, E. N., and Landsman, D. (1995) Nucleic Acids Res. 23, 2685-2691 [Abstract]
  12. Richmond, T. J., Finch, J. T., Rushton, B., Rhodes, D., and Klug, A. (1984) Nature 311, 532-536 [Medline] [Order article via Infotrieve]
  13. Garcia-Ramirez, M., Dong, F., and Ausio, J. (1992) J. Biol. Chem. 267, 19587-19595 [Abstract/Free Full Text]
  14. Fletcher, T. M., and Hansen, J. C. (1995) J. Biol. Chem. 270, 25359-25362 [Abstract/Free Full Text]
  15. Tatchell, K., and van Holde, K. E. (1977) Biochemistry 24, 5295-5303
  16. Bustin, M., and Reeves, R. (1996) Prog. Nucleic Acid Res. Mol. Biol. 54, 35-99 [Medline] [Order article via Infotrieve]
  17. Bustin, M. (1979) Curr. Top. Microbiol. Immunol. 88, 105-142 [Medline] [Order article via Infotrieve]
  18. Stollar, B. D., and Ward, M. (1970) J. Biol. Chem. 245, 1261-1266 [Abstract/Free Full Text]
  19. Muller, S., Erard, M., Burggraf, E., Couppez, M., Sautière, P., Champagne, M., and Van Regenmortel, M. H. V. (1982) EMBO J. 1, 939-944 [Medline] [Order article via Infotrieve]
  20. Muller, S., Himmelspach, K., and Van Regenmortel, M. H. V. (1982) EMBO J. 1, 421-425 [Medline] [Order article via Infotrieve]
  21. Hacques, M.-F., Muller, S., De Murcia, G., Van Regenmortel, M. H. V., and Marion, C. (1990) Biochem. Biophys. Res. Commun. 168, 637-643 [Medline] [Order article via Infotrieve]
  22. Turner, B. M. (1991) J. Cell Sci. 99, 13-20 [Medline] [Order article via Infotrieve]
  23. Perry, C. A., Dadd, C. A., Allis, C. D., and Annunziato, A. T. (1993) Biochemistry 32, 13605-13614 [Medline] [Order article via Infotrieve]
  24. Strosberg, A. D. (1983) Springer Semin. Immunopathol. 6, 67-78 [Medline] [Order article via Infotrieve]
  25. Nothacker, K. D., and Hildebrandt, A. (1985) Eur. J. Cell Biol. 39, 278-282
  26. Loidl, P., and Gröbner, P. (1987) J. Biol. Chem. 262, 10195-10199 [Abstract/Free Full Text]
  27. Helliger, W., Lindner, H., Hautplorenz, S., and Puschendorf, B. (1988) Biochem. J. 255, 23-27 [Medline] [Order article via Infotrieve]
  28. Von Holt, C., Brandt, W. F., Greyling, H. J., Lindsey, G. G., Retief, J. D., Rodrigues, J. de A., Schwager, S., and Sewell, B. T. (1989) Methods Enzymol. 170, 431-523 [Medline] [Order article via Infotrieve]
  29. Muller, S., and Van Regenmortel, M. H. V. (1989) Methods Enzymol. 170, 431-523 [Medline] [Order article via Infotrieve]
  30. Olmsted, J. B. (1981) J. Biol. Chem. 256, 11955-11957 [Abstract/Free Full Text]
  31. Thiriet, C., and Albert, P. (1995) Electrophoresis 18, 357-361
  32. Laemmli, U. K. (1970) Nature 227, 680-685 [Medline] [Order article via Infotrieve]
  33. Harlow, E., and Lane, D. (1988) Antibodies, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
  34. Hayes, J. J., and Wolffe, A. P. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 6415-6419 [Abstract]
  35. Hayes, J. J. (1996) Biochemistry 35, 11931-11937 [CrossRef][Medline] [Order article via Infotrieve]
  36. Banères, J.-L., Essalouh, L., Jariel-Encontre, I., Mesnier, D., Garrod, S., and Parello, J. (1994) J. Mol. Biol. 243, 48-59 [CrossRef][Medline] [Order article via Infotrieve]
  37. Pruss, D., Hayes, J. J., and Wolffe, A. (1995) BioEssays 17, 161-170 [Medline] [Order article via Infotrieve]
  38. Pruss, D., Bartholomew, B., Persinge, J., Hayes, J. J., Arents, G., Moudrianakis, E. N., and Wolffe, A. P. (1996) Science 274, 614-617 [Abstract/Free Full Text]
  39. Turner, B. M. (1981) Eur. J. Cell Biol. 24, 266-274 [Medline] [Order article via Infotrieve]
  40. Mendelson, E., and Bustin, M. (1984) Biochemistry 17, 3459-3466
  41. Muller, S., Jockers-Wretou, E., Sekeris, C. E., Van Regenmortel, M. H. V., and Bautz, F. A. (1985) FEBS Lett. 182, 459-464 [CrossRef][Medline] [Order article via Infotrieve]
  42. Dousson, S., Gorka, C., Gilly, C., and Lawrence, J.-J. (1989) Eur. J. Immunol. 19, 1123-1129 [Medline] [Order article via Infotrieve]
  43. Karantza, V., Freire, E., and Moudrianakis, E. N. (1996) Biochemistry 35, 2037-2046 [CrossRef][Medline] [Order article via Infotrieve]

©1997 by The American Society for Biochemistry and Molecular Biology, Inc.