COMMUNICATION:
Molecular Mechanism and Functional Significance of the MinK Control of the KvLQT1 Channel Activity*

(Received for publication, April 8, 1997, and in revised form, May 12, 1997)

Georges Romey Dagger , Bernard Attali §, Christophe Chouabe Dagger par , Ilane Abitbol §, Eric Guillemare Dagger **, Jacques Barhanin Dagger and Michel Lazdunski Dagger Dagger Dagger

From the Dagger  Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, 660 route des Lucioles, Sophia Antipolis, 06560 Valbonne, France and the § Weizmann Institute of Science, Department of Neurobiology, 76100 Rehovot, Israel

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS AND DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES


ABSTRACT

The very slowly activating delayed rectifier K+ channel IKs is essential for controlling the repolarization phase of cardiac action potentials and K+ homeostasis in the inner ear. The IKs channel is formed via the assembly of two transmembrane proteins, KvLQT1 and MinK. Mutations in KvLQT1 are associated with a long QT syndrome that causes syncope and sudden death and also with deafness. Here, we show a new mode of association between ion channel forming subunits in that the cytoplasmic C-terminal end of MinK interacts directly with the pore region of KvLQT1. This interaction reduces KvLQT1 channel conductance from 7.6 to 0.58 picosiemens. However, because MinK also reveals a large number of previously silent KvLQT1 channels (× 60), the overall effect is a large increase (× 4) in the macroscopic K+ current. Conformational changes associated with the KvLQT1/MinK association create very slow and complex activation kinetics without much alteration in the deactivation process. Changes induced by MinK have an essential regulatory role in the development of this K+ channel activity upon repetitive electrical stimulation with a particular interest in tachycardia.


INTRODUCTION

Delayed K+ rectifier channels initiate the repolarization that terminates the plateau phase of the action potential. The delayed rectifier K+ current is the addition of two components, a rapidly activating one, which is called IKr, and a very slowly activating current called IKs (1). Cardiac arrhythmias, based on abnormal repolarization, are visualized as a prolonged QT interval on an electrocardiogram. Congenital long QT (LQT)1 is an inherited disease characterized by prolonged ventricular repolarization that causes syncope and sudden death due to ventricular arrhythmia (2). The LQT syndrome is genetically heterogeneous with at least four chromosomal loci (LQT1 to LQT4) implicated in the disease. One of them, the LQT2 locus, corresponds to mutations in the HERG gene that encodes the rapidly activating delayed rectifier K+ channel generating IKr. Another one, the LQT1 locus, encodes a K+ channel protein, KvLQT1, that associates with another small transmembrane protein known as MinK, to generate the slowly activating K+ channel IKs (3, 4). Expression of IKs is not limited to the heart. KvLQT1 as well as MinK are also expressed in many other organs such as kidney and the stria vascularis of the inner ear (5). Some human mutations of the KVLQT1 gene lead to the Jervell-Lange-Nielsen syndrome (6). Patients suffering from this syndrome not only exhibit a long QT wave interval but also profound deafness from birth. On the other hand, mice carrying a null mutation on the MinK gene also display profound inner ear dysfunction associated with drastically altered K+ secretion into the endolymph of the inner ear leading to hair cell degeneration (7). Thus, the KvLQT1/MinK assembly forms a K+ channel that has a key electrogenic role in ventricular repolarization and a key secretory role in the control of endolymph homeostasis associated with normal hearing.

KvLQT1 has the classical structure of a K+ channel protein with six transmembrane regions and one pore structure that is known to confer K+ permeability (8), whereas MinK is a small protein (129 amino acids in the mouse) with a single transmembrane domain (9, 10), which serves as an essential modulator of the KvLQT1 subunit (3, 4, 11). This paper shows that MinK has unique properties of interaction with KvLQT1. This peculiar mode of interaction confers functional properties to the IKs channel that probably have very important physiopathological implications


EXPERIMENTAL PROCEDURES

Electrophysiology

Transfection of COS cells has been previously described (3). The whole cell, cell-attached, and outside-out configurations of the patch-clamp technique were used (12). The external solution at pH 7.4 contained (in mM): 140 NaCl, 5 KCl, 1 CaCl2, 2 MgCl2, 10 HEPES/NaOH. Pipette solutions contained either the external medium (cell-attached) or an internal solution at pH 7.3 with (in mM): 140 KCl, 2 MgCl2, 10 HEPES/KOH, 2 EGTA.

The mean current-variance analysis on COS cells expressing KvLQT1/MinK channels was performed using the Biopatch software (Bio-Logic, Grenoble, France). Currents were sampled at 1 kHz and low pass filtered at 150 Hz. Methods of cRNA injection in Xenopus oocytes and electrophysiological recordings have been described (10).

Yeast Two-hybrid Interaction Assay

The fragments encompassing part of the N-terminal domain of human MinK (MinKN, aa 11-38) and the entire MinK C terminus (MinKC, aa 67-129) were amplified by polymerase chain reaction using the Vent DNA polymerase (Biolabs) and subcloned in fusion with the GAL4 DNA-binding domain of the yeast vector pAS2 (CLONTECH) into the SmaI/PstI and BamHI/PstI cloning sites, respectively. The domains of KvLQT1 spanning the entire N terminus (KvLQT1N, aa 1-64), the pore (KvLQT1P, aa 218-259) and the entire C terminus (KvLQT1C, aa 290-604) were amplified by polymerase chain reaction and subcloned in fusion with the GAL4 activation domain of the yeast vector pGAD 424 (CLONTECH) into the EcoRI/BamHI cloning sites. All constructs were verified by sequencing. The two types of hybrid plasmids were transformed into the yeast strain CG-1945 using the lithium acetate method, and transformants were grown on synthetic medium lacking Leu, Trp, and His in the presence of 5 mM 3-aminotriazole to inhibit basal levels of HIS3 expression. Primary HIS+ transformants were then tested for beta -galactosidase reporter gene activity using both a filter and a liquid assay. The beta -galactosidase units were calculated according to the CLONTECH protocol. The pore of KvLQT1 and the corresponding N and C termini did not exhibit any beta -galactosidase activity when cotransformed with the pAS2 vector alone, with pVA3 a plasmid encoding the murine p53, or with pLAM5' a vector encoding the human lamin C (not shown).

Affinity Chromatography

A series of glutathione S-transferase fusion proteins corresponding to C and N termini of MinK, MinKC (aa 67-129), and MinKN (aa 11-38) and to three different domains of KvLQT1, including the pore, the C and N termini, KvLQTP (aa 218-259), KvLQTC (aa 290-604), and KvLQTN (aa 1-64) were expressed in Escherichia coli (BL21) and purified on glutathione-Sepharose beads. Sf9-infected cells were homogenized and solubilized in lysis buffer (50 mM Tris-HCl buffer, pH 7.4, containing 5 mM EDTA, 1 mM phenylmethylsulfonyl fluoride, 5 mM benzamidine, 10 µg/ml leupeptin, 10 µg/ml aprotinin, 1% Triton X-100) and centrifuged at 21,000 × g for 20 min at 4 °C. The solubilized extracts (20 µg) were incubated in lysis buffer for 90 min at 4 °C with 10 µg of purified fusion protein previously bound to 30 µl of glutathione-Sepharose. Samples were washed three times (1 ml each wash) with lysis buffer. Bound proteins were eluted with sample buffer, resolved by SDS-polyacrylamide gel electrophoresis, and subjected to Western blotting. Immunodetection of MinK and Kv1.5 was carried out using rabbit polyclonal anti-MinK and anti-Kv1.5 antibodies, whereas that of Kv2.2 was performed by using monoclonal anti-FlagM2 antibodies.

Simulation

Kinetic schemes of Fig. 4 were simulated using "Mathematica" Software (Wolfram Research).


Fig. 4. Models of KvLQT1 and KvLQT1/MinK channels. A, KvLQT1 channel. C0 and C1, closed states; O, open state. B, KvLQT1/MinK channel. The C-terminal part of MinK being in the inner mouth of the pore (MK conformation) or inside the pore (MK* conformation). States are: closed (C0MK and C1MK), blocked (OMK and C1MK*), partially open (OMK*). C, simulation of the models using the indicated rate constant values. D, simulation of the experiment shown in Fig. 1E. E, extension of the model; MK (bullet ) or MK* (*) associated with 4 KvLQT1 subunits. In the states diagram, the channel is open if all MinK are in the MK* conformation and blocked if only one is in the MK conformation. F, probability of the channel to integrate 0, 1, 2, 3, or 4 MinK as a function of MinK concentration. G, time course of activation in the same Xenopus oocyte at a different time scale of stimulation. Voltage-clamp to +30 mV from -80 mV. H, simulation at two consecutive time scales of the fastest (KvLQT1/MinK) and the slowest (KvLQT1/4MinK) activation kinetics.
[View Larger Version of this Image (92K GIF file)]


RESULTS AND DISCUSSION

Although the activation kinetics and level of K+ channel expression of KvLQT1 and KvLQT1/MinK are very different, the deactivation kinetics are comparable with both tau deact ~500 ms at -50 mV (Fig. 1, A-C). The open state accumulation that provokes the increasing successive K+ current responses upon repetitive stimulations cannot be obtained with KvLQT1 alone but necessitates the presence of MinK in the channel complex (Fig. 1, D-E). With a stimulation duration in the range of the cardiac action potential (300 ms), a 100% enhancement of the outward current elicited by consecutive depolarizing pulses is observed when the stimulation rate is 120 stimulations/min (Fig. 1F), whereas this cumulative effect is totally absent at 40 stimulations/min. The frequency-cumulative current increase relationship (Fig. 1G) is centered on the value of 80 stimulations/min, a value close to the human heart beat frequency. Thus, MinK association with KvLQT1 potentially explains the role of the channel in the rate-dependent shortening of normal cardiac action potentials preventing the myocardium from premature excitation (13, 14). A dysfunction of IKs in patients with inherited LQT syndrome linked to the LQT1 locus will decrease or suppress this large K+ current, which develops at high stimulation frequencies i.e. with tachycardia. It can be predicted that hearts from these patients would be unable to protect themselves against arrhythmias when sympathetic activity increases with emotional and/or physical stress that precipitates life-threatening events. It makes sense that the most efficient pharmacological strategy in these patients is to prevent tachycardia with beta -blockers (15).


Fig. 1. Transient expressions of KvLQT1 and KvLQT1/MinK in transfected COS cells. Fast activating (A) KvLQT1 and slow activating KvLQT1/MinK (B) channels in COS cells. Voltage pulses from -80 to +50 mV in 10-mV steps. Tail currents at -40 mV. C, voltage-dependent kinetics of deactivation fitted with a single exponential. Inset, stimulation protocol. D and E, pairs of 300-ms pulses from -80 to +30 mV separated by time intervals from 0 to 330 ms in 30-ms increments. The accumulation process was absent for KvLQT1 (D) and present for KvLQT1/MinK (E). F, frequency-dependent enhancement of KvLQT1/MinK current. The protocol was 300-ms pulses to +30 mV from -80 mV at different rates. G, maximum amplitude of the current as a function of the rate of stimulation (three experiments).
[View Larger Version of this Image (33K GIF file)]

At the unitary channel level, the effects of the association MinK-KvLQT1 are also spectacular. COS cells transfected with KvLQT1 only express a small number of channels (1 or 2) in each active patch (n = 10) with a single channel conductance 7.6 ± 0.7 pS (Fig. 2, A-C). Single channel currents cannot be easily detected in most patches (n = 40) from COS cells expressing KvLQT1/MinK. In these patches, the recorded current mimics the global current (Fig. 2D, a). Therefore, variance analysis was used to estimate both the number of active channels and the value of the unitary current. Fig. 2D (b) illustrates this method applied to an outside-out patch. Patches typically contained 50-100 channels with a unitary conductance of 0.58 ± 0.14 pS (n = 25) (Fig. 2, D and E). Because the variance-mean current analysis hypothesizes that current fluctuations are directly related to channels flickering between closed and open states, it is not surprising that the parabola fits indicate the existence of numerous channels of small conductance. Furthermore, Fig. 2 (E and F) provide convergent results in favor of the validity of the parabola fits: (i) the single channel current-voltage curve intersects the voltage axis at the K+ equilibrium potential (approx  -80 mV) (Fig. 2E), and the calculated number of channels is near constant for the same patch. (ii) A single channel analysis has also been carried out, in rare patches displaying "visible" single channel activities. Elementary conductances and number of channels calculated by the two methods, i.e. variance analysis and amplitude histograms, have given similar results (0.6 pS, two channels). In the Xenopus oocyte, noise analysis on KvLQT1/MinK containing macropatch yields the same unitary conductance (0.52 ± 0.2 pS, n = 20) with a mean number of active channels of about 1000 (not shown). These results are in agreement with those obtained from cardiac patches, suggesting that IKs is due to the activity of a high density very small K+ conductance channel (16, 17).


Fig. 2. Analysis of detectable (KvLQT1 channel) and nondetectable (KvLQT1/MinK channel) unitary currents in COS membrane patches. A, single KvLQT1 channel activities at different potentials relative to the resting potential (RP). B, amplitude histogram of unitary currents at +60 mV/RP. C, current-voltage relationship (mean values from three patches). D, a, KvLQT1/MinK channel, outside-out patch, representative K+ current response to step depolarization to +40 mV. b, variance-mean current plot and fit with the parabolic function: sigma 2 = i·I(t- I(t)2/N, where sigma 2 = variance of the current, i = unitary current, and N = number of functional channels (n = 90, i = 0.076 pA). E, current-voltage relationship for the unitary currents. bullet , mean from 30 cell-attached patches; black-diamond  and black-square, from patches shown in D and F, respectively. F, a, cell-attached patch with detectable slowly activating K+ currents in response to a step depolarization to +100 mV relative to RP followed by a return to +40 mV. b, noise analysis. c, amplitude histogram from the same data.
[View Larger Version of this Image (42K GIF file)]

The large increase in K+ current following association of MinK with KvLQT1 results from the melange of two factors: a large reduction in the unitary channel conductance overcompensated by a larger increase in the number of functional channels. The 4-fold increase of the current density in COS cells expressing KvLQT1/MinK (43.1 ± 4 pA/pF, n = 31 at +30 mV) as compared with cells expressing KvLQT1 alone (10.2 ± 1.3 pA/pF, n = 29) reflects a 60-fold increase of the number of functional K+ channels. Most KvLQT1 channels are "nonfunctional" or in a "silent" state with a low probability of opening. The association of MinK converts them into small conductance channels but with a high open state probability.

A series of experiments (Fig. 3) demonstrates the interaction of the MinK C-terminal domain, known to be an essential element for the KvLQT1/MinK expression (3, 11), with structural elements within or close to the KvLQT1 pore. First, the yeast two-hybrid assay shows that the MinK C terminus interacts strongly with the pore of KvLQT1 (Fig. 3A). It does not interact significantly with either the entire N terminus or the entire C terminus of the KvLQT1 channel protein. The N terminus of MinK fails to interact with any hydrophilic domain (N-terminal, C-terminal, or pore) of KvLQT1 (not shown). Second, affinity chromatography of MinK to the various domains of the KvLQT1 protein was performed. The whole MinK protein was produced in Sf9 insect cells infected with recombinant baculovirus (18). Detergent cell extracts were incubated with the different domains of KvLQT1 produced as glutathione S-transferase fusion proteins in E. coli and bound to glutathione-Sepharose beads. The retained proteins were resolved by SDS-polyacrylamide gel electrophoresis and Western blotted using polyclonal anti-MinK antibodies (18). Again, the results show that only the pore region of KvLQT1 specifically associates with MinK (Fig. 3B). The two-hybrid and the affinity chromatography assays have been used in several studies designed to map domains mediating protein-protein interactions in K+ channels (19, 20). However, interactions involving other domains of MinK or KvLQT1 may exist that have not been identified in the present study.


Fig. 3. Interaction of the C-terminal domain of MinK with the P domain of KvLQT1. A, inset, the various domains of MinK (bait) and KvLQT1 (prey) used in the yeast two-hybrid assay are in bold. Inset, 1, MinK N terminus; 2, MinK C terminus; 3, KvLQT1 N terminus; 4, KvLQT1 P; 5, KvLQT1 C terminus. Main panel, two-hybrid interactions quantified by the beta -galactosidase assay. Data are means ± S.E. (n = 3). B, immunodetection of MinK following affinity chromatography with the N-terminal, C-terminal, or pore domains of KvLQT1. The two bands at 23 and 27 kDa represent glycosylated forms of the MinK protein expressed in Sf9 cells as demonstrated by deglycosylation treatments described in Ref. 18.
[View Larger Version of this Image (22K GIF file)]

Both electrophysiological and biochemical data have been used to propose a minimal model that could help explain how the KvLQT1/MinK association leads to drastic changes in properties of the KvLQT1 channel such as activation kinetics and unitary conductance while preserving K+ selectivity and deactivation kinetics (Fig. 4). MinK first binds to the outer shell of the KvLQT1 channel, probably via its transmembrane domain. This step provides a closer positioning of the C-terminal domain of MinK to the pore of KvLQT1. Once the KvLQT1 channel reaches the open conformation (O), the C-terminal domain of MinK enters and binds to the pore (OMK). This leads to a total occlusion that is later transformed into a partial occlusion resulting in a narrower pore (OMK*) that creates an additional barrier to K+ mobility and drastically reduces the unitary conductance. The total pore occlusion produced by MinK before relaxation to a partial occlusion is supported by the fact that the normal 7.6 pS KvLQT1 conductance was never recorded with the KvLQT1/MinK channel before observing the small conductance behavior. The slow kinetics of activation reflect the conformational change (OMK right-arrow OMK*) leading to the partial opening of the pore. The difficulty for the channel to close when occupied by the C-terminal end of MinK, the "foot-in-the-door" process (21), leads to the accumulation of open channels (OMK*) and to an increase in the number of functional channels. Actually, long open times (>1 s) are observed in the few patches with detectable unitary currents (Fig. 2F, a). Assigning an arbitrary value of 1 to the rate constant of the transition between the closed states C0 and C1, one can then set all the other rate constants (Fig. 4) for a quantitative treatment of the model. A computer simulation provides a satisfactory fit of the key current properties of KvLQT1/MinK, i.e. the slow activation kinetics, the higher level of K+ channel expression, the unchanged rates of deactivation and the frequency-dependent accumulation as in Fig. 1E. An extension of the model is presented in Fig. 4E in which a tetramer of KvLQT1 can bind from 1 to 4 MinK subunits depending on the concentration of MinK in the membrane. This model explains (i) the complex kinetic behavior of the slow KvLQT1/MinK channel after a long-lasting depolarization (compare Fig. 4, G and H) and (ii) previous results demonstrating that the kinetics of activation of the slow K+ channel formed in the Xenopus oocyte depends on the levels of the MinK protein present in the membrane (22, 23). Thus, MinK appears to be a regulatory protein that finely tunes the KvLQT1 channel activity in a concentration-dependent manner. This property is supported by results shown in Fig. 5. The rapidly activating KvLQT1 currents can be first expressed in Xenopus oocytes injected with KvLQT1 cRNA alone and then converted into slowly activating ones following expression of MinK 24 h later. This modification of pre-existing channels by the association of the non-pore-forming MinK subunit clearly contrasts with the situation described for classical K+ channel beta -subunits (24) where the association with a subunits only occurs during the translation process in the endoplasmic reticulum (25, 26).


Fig. 5. Time course of the MinK functional expression. A, typical current traces evoked at +30 mV in Xenopus oocyte injected with KvLQT1 cRNA (KvLQT1, 48 h) and then reinjected with MinK cRNA for 24 h (+MinK, 24 h). B, bar graph showing the mean peak currents elicited by voltage steps to +30 mV with delayed injection of MinK cRNA. Data are means ± S.E. (n = 4-9).
[View Larger Version of this Image (24K GIF file)]

The effect of a change in the MinK concentration on KvLQT1/MinK kinetics is of a particular physiological interest because the level of expression of MinK is known to be altered during development (10) and by hormones such as oestrogens (27). The latter process being a potential explanation for sex differences in cardiac LQT and vulnerabilities to "torsades de pointes" (28).


FOOTNOTES

*   This work was supported in part by the Centre National de la Recherche Scientifique, Ministère de l'Enseignement Supérieur et de la Recherche Contract MESR ACC SV9 9509113, and the Association Française contre les Myopathies and by grants from Israel Cancer Research Fund (Research Cancer Development Award) and the Israel Academy of Science (to B. A.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
   Incumbent of the Philip Harris and Gerald Ronson Career Development Chair.
par    Recipient of a grant from the Association Française contre les Myopathies.
**   Present address: Sanofi Recherche, Département Cardio-Vasculaire, 371 rue du Prof. Joseph Blayac, 34184 Montpellier cedex 04, France.
Dagger Dagger    To whom correspondence should be addressed. Tel.: 33-4-93-95-77-02; Fax: 33-4-93-95-77-04; E-mail: ipmc{at}unice.fr.
1   The abbreviations used are: LQT, long QT; aa, amino acid(s); pS, picosiemens; RP, resting potential.

ACKNOWLEDGEMENTS

We give special thanks to Dr A. Patel for reading the manuscript. We are very grateful to M. Jodar and D. Doume for expert technical assistance.


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