(Received for publication, January 3, 1997, and in revised form, March 5, 1997)
From the Department of Biochemistry and the
¶ Division of Dermatology, Vanderbilt University School of
Medicine, Nashville, Tennessee 37232
In mitogenic signaling pathways, Shc participates in the growth factor activation of Ras by interacting with activated receptors and/or the Grb-2·Sos complex. Using several experimental approaches we demonstrate that Shc, through its SH2 domain, forms a complex with the cytoplasmic domain of cadherin, a transmembrane protein involved in the Ca2+-dependent regulation of cell-cell adhesion. This interaction is demonstrated in a yeast two-hybrid assay, by co-precipitation from mammalian cells, and by direct biochemical analysis in vitro. The Shc-cadherin association is phosphotyrosine-dependent and is abrogated by addition of epidermal growth factor to A-431 cells maintained in Ca2+-free medium, a condition that promotes changes in cell shape. Shc may therefore participate in the control of cell-cell adhesion as well as mitogenic signaling through Ras.
Shc (1, 2) is an adaptor protein and tyrosine kinase substrate
that contains an N-terminal phosphotyrosine-binding
(PTB)1 domain (3), a central collagen-like
region that contains three tyrosine phosphorylation sites (4-6), and a
C-terminal src homology 2 (SH2) domain (see Fig.
1A). The SH2 domain recognizes phosphotyrosine but in a
manner mechanistically and structurally distinct from the PTB domain.
Although Shc is known to participate in Ras activation by growth
factors, the properties of Drosophila Shc have suggested participation in other, unknown pathways (7). This is likely to occur
through protein-protein associations because Shc has no catalytic
function. In growth factor-dependent signal transduction, Shc phosphotyrosine residues mediate association with the Grb-2·Sos complex involved in Ras activation (8), whereas the PTB domain recognizes NPXpY sequences in several autophosphorylated
growth factor receptors and other tyrosine phosphorylated molecules
(3). Nonphosphorylated residues within the collagen-like region of Shc
mediate an interaction with -adaptin, a coated-pit component (9)
implicated in the endocytosis of growth factor receptors. The
functional significance of this interaction is, as of now, not known.
Whereas the identity of association partners with the SH2 domain of Shc
is unclear, over-expression of the Shc SH2 domain attenuates growth
factor-induced mitogenesis in a dominant-negative manner (10-12). We
present evidence that this SH2 motif mediates an interaction between
Shc and cadherins, transmembrane cell-cell adhesion receptors,
suggesting a function of Shc in the maintenance of cell-cell adhesion
and cell shape.
The antibodies used were rabbit IgG fractions to phosphotyrosine (Transduction Laboratories, horseradish peroxidase-coupled), to cadherin (pan-cadherin, ICOS Corp.), and to Shc for Western blotting (Transduction Laboratories). For the immunoprecipitation of Shc, antiserum to recombinant p52 Shc was produced. The coding sequence for the 52-kDa form of Shc was cloned into the pAcHLT-B baculovirus transfer vector (Pharmingen, Corp.) and transferred into sf9 insect cells. The His-tagged Shc protein was overexpressed in High 5 insect cells and purified via Ni2+ affinity chromatography (Qiagen). 150 µg of p52Shc from the 150 mM imidazole elution fraction was used as immunogen to subcutaneously inject a rabbit. Following three booster injections, immune serum was harvested. Sodium orthovanadate was purchased from Fisher, and hydrogen peroxide was from Sigma.
Yeast Two-hybrid ScreenThe SH2 domain (residues 373-469) of Shc (1) was fused to the LexA DNA-binding domain and used as bait to screen a mouse 10-day embryo library fused to VP16 transcription activation domain. The yeast two-hybrid assay with this library was carried out essentially as described elsewhere (13, 14) except that to tyrosine phosphorylate library proteins a constitutive active form of c-Src under the control of ADH1 promoter (15) was cloned into the NaeI site of the same bait plasmid containing the LexA-Shc-SH2 construct (pBTM116 Shc-SH2+Src).
ImmunoprecipitationA-431 and NIH 3T3 cells were grown in Dulbecco's modified Eagle's medium with 10% calf serum at 37 °C under 5% CO2. Upon reaching confluence and growth factor treatment (where indicated), cells were lysed in TGH buffer (1% Triton X-100, 10% glycerol, 50 mM Hepes, pH 7.2, and 100 mM NaCl) supplemented with 10 ng/ml leupeptin, 10 ng/ml aprotinin, 544 µM iodacetamide, 1 mM phenylmethylsulfonyl fluoride, and 1 mM sodium vanadate. 5 µl preimmune serum or serum containing p52Shc antibody were added to 500 µg of cell lysate, and after incubation at 4 °C for 2 h, immunocomplexes were collected by addition of protein A conjugated to Sepharose beads (Sigma). After washing with immunoprecipitation washing buffer (20 mM Hepes, pH 7.2, 100 mM NaCl, 10% glycerol, and 0.1% Triton X-100), bound proteins were eluted with SDS sample buffer, subjected to SDS-PAGE and transferred to nitrocellulose filters. The filters were blocked with TBSTB buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Tween 20, and 3% bovine serum albumin) and incubated with primary antibody in TBSTB buffer for 2 h at room temperature. The filters were washed with TBST buffer without 3% bovine serum albumin, followed by incubation with protein A-horseradish peroxidase (Zymed) in TBSTB buffer for 1 h. The filters were then washed with TBST buffer, incubated with ECL working solution (Amersham Corp.) for 1 min, and exposed to x-ray film.
In Vitro Tyrosine Phosphorylation of GST-N-Cadherin C TerminusClone S24 corresponding to the N-cadherin intracellular domain (residue 792-906) was fused in-frame to GST, expressed in Escherichia coli, and purified on glutathione-Sepharose 4B beads according to the manufacturer's manual (Pharmacia Biotech Inc., pGEX-5X-1 vector). Purified c-Src (Upstate Biotechnology Inc.) was used in kinase assays according to the manufacturer's instructions.
Gel Overlay AssayThe nitrocellulose filter was treated with 6 M guanidine hydrochloride to denature proteins at 4 °C for 10 min in Hyb buffer (20 mM Hepes, pH 7.6, 75 mM KCl, 0.l mM EDTA, 2.5 mM MgCl2, 1 mM dithiothreitol, and 0.05% Nonidet P-40), and proteins were renatured at 4 °C by five successive dilutions (40 min each) of the guanidine HCl to a final concentration of 0.185 M in the same buffer. Following two 30-min washes with Hyb buffer, the filter was blocked by 30-min incubations in 5 and 1% milk in Hyb buffer. The filter was incubated with purified p52Shc overnight at 4 °C, washed with Hyb buffer, and blotted with anti-Shc (Transduction Laboratory).
To identify tyrosine phosphorylated
molecules that recognize the SH2 domain of Shc, a modified yeast
two-hybrid screen was performed in a system that included the Shc SH2
domain fused to the LexA DNA-binding domain, a mouse embryo library
(13, 14) fused to the VP16 transactivation domain, and a constitutively active form of the tyrosine kinase c-Src regulated by the ADH1 promoter
(15), because SH2 interacting molecules are expected to contain
phosphotyrosine. One positive clone, clone S24 (Fig. 1B), contained sequences that when translated
correspond to residues 792-906 within the cytoplasmic domain of mouse
N-cadherin (Fig. 1B). Cadherins are transmembrane proteins
that regulate cell-cell adhesion in a
Ca2+-dependent manner (16). The cytoplasmic
domains of the three major cadherins are relatively conserved in
sequence, particularly within the region corresponding to clone S24
(Fig. 1C). Clone S24 was subsequently retested in the
two-hybrid assay in the presence and the absence of c-Src. No
interaction of S24 with the Shc SH2 motif was detected in the absence
of c-Src. Also, the central region of PLC-1, which contains two SH2
domains, did not, when substituted for the Shc SH2 domain, interact
with clone S24 in the presence of c-Src.2
These results indicate a putative recognition of cadherin by the SH2
domain of Shc.
To determine whether the
native Shc protein interacts with cadherin, co-immunoprecipitation
assays were performed with A-431 and NIH 3T3 cells. The results shown
in Fig. 2 demonstrate the specific co-precipitation of
cadherin in Shc immunoprecipitates obtained from both cell types and in
the absence of exogenous growth factor stimulation. Therefore, under
typical cell culture conditions the association of Shc and cadherin is
constitutive and likely dependent on the basal activity of tyrosine
kinases.
The extracellular domain of cadherins binds Ca2+ and mediates Ca2+-dependent cell-cell association. This recognition event involves the lateral dimerization of cadherin molecules (17-19) and the homophilic association of cadherin extracellular domains between adjacent cells (16). Cell-cell interaction then transmits biochemical signals through the cadherin cytoplasmic domain to effector molecules, such as the catenins, that bring about changes in actin cytoskeletal structure.
When placed in Ca2+-free medium, adherent and spread-out
A-431 cells undergo a rapid morphological change to a round morphology following the addition of epidermal growth factor (EGF) (20). Given the
Ca2+ dependence of cadherin function in cell-cell
association, we examined the state of Shc association with cadherin in
the presence or the absence of extracellular Ca2+ and EGF.
The results presented in Fig. 3A demonstrate
that the addition of EGF to A-431 cells in Ca2+-containing
medium has no significant influence on cadherin co-precipitation with
Shc (lanes 1 and 2). However, when the cells were
placed in a Ca2+-free medium and EGF was added, a large
decrease in cadherin association with Shc is detected (lanes
3 and 4). Because the incubation period for this
experiment was 30 min and cell rounding occurs within this time, the
observed loss of cadherin association with Shc could be a consequence
of the change in cell shape that occurs when EGF is added to cells in
the Ca2+-free medium. Therefore, under the same conditions
A-431 cells were analyzed for Shc-cadherin association at much earlier
times (1-30 min). As shown in Fig. 3B, cadherin association
with Shc was significantly decreased 1 min (lane 2) after
the addition of EGF to A-431 cells in this Ca2+-free
medium. Hence, cadherin interaction with Shc is disrupted prior to
observable changes in cell morphology. However, the biochemical mechanism underlying dissociation of the Shc-cadherin complex under
these experimental conditions is not known.
In this assay system, prolonged incubation in Ca2+-free medium without EGF does decrease Shc-cadherin association to a moderate extent. However, the addition of EGF dramatically enhances the rapidity and extent of complex dissociation. The low level of cadherin that remains detectable in Shc immunoprecipitates obtained from cells treated with EGF in Ca2+-free medium (Fig. 3B, lanes 4 and 6), is due, at least in part, to cadherin present nonspecifically in precipitates obtained from A-431 cells with preimmune serum (Fig. 2).
The Src dependence of the two-hybrid assay results and the known
properties of SH2 domains would predict that the SH2 domain of Shc
recognizes phosphotyrosine within the cytoplasmic domain of cadherin.
Although none of the six tyrosine residues in the cadherin cytoplasmic
domain (Fig. 1C) have been identified as phosphorylation
sites, Tyr851 and Tyr883 conform to the
consensus recognition sequence of the Shc SH2 domain (pY with L/I/M at
the +3 position), as deduced from random peptide libraries (21) and the
T cell receptor chain peptide used to solve the solution structure
of a liganded Shc SH2 domain (22). Others have reported the presence of
phosphotyrosine at low levels on cadherin (23-25), although cadherin
is not generally cited as a tyrosine phosphorylated protein. Perhaps
complicating phosphotyrosine detection is the reported association of
phosphotyrosine phosphatases with cadherin (25-27). We have assayed
A-431 cells for the presence of phosphotyrosine on cadherin (Fig.
4A). In the presence but not the absence of
pervanadate, an inhibitor of phosphotyrosine phosphatases, tyrosine
phosphorylation of cadherin is more readily detected (lane
2).
If pervanadate enhances cadherin tyrosine phosphorylation and Shc association with cadherin is phosphotyrosine-dependent, then it is expected that pervanadate will increase the level of Shc-cadherin complexes. The data in Fig. 4B show that pervanadate substantially increases the amount of cadherin present in Shc immunoprecipitates (lanes 1 and 2) and the level of Shc, particularly the p52 isoform, detectable in cadherin immunoprecipitates (lanes 3 and 4). These data, therefore, are consistent with the association of Shc and cadherin in a phosphotyrosine-dependent manner. Because EGF stimulates Shc tyrosine phosphorylation (1) but does not enhance Shc association with cadherin (Fig. 3B), the pervanadate influence on this association is likely due to the increased phosphotyrosine on cadherin.
Shc Association with Tyrosine Phosphorylated Cadherin in VitroTo determine whether Shc interacts directly with cadherin
and to resolve the issue of whether cadherin must be tyrosine
phosphorylated to affect this association, the in vitro
experiments described in Fig. 5 were performed. The
clone S24 sequence corresponding to residues 702-906 of N-cadherin was
expressed as a GST fusion protein and purified by absorption on
glutathione-Sepharose. As a control, GST was absorbed to the
glutathione matrix. Aliquots of GST and GST-cadherin were then
incubated with c-Src in the presence or the absence of ATP, eluted from
the column with glutathione, separated by SDS-PAGE, and, following
transfer to nitrocellulose, blotted with anti-phosphotyrosine (Fig.
5A) or anti-GST (Fig. 5B). The results show
clearly that GST-cadherin is tyrosine phosphorylated in the presence of
c-Src and ATP.
In a parallel experiment, following incubation with c-Src and/or ATP, GST and GST-cadherin were transferred to filters, denatured in 6 M guanidine HCl, and then gradually renatured. The filters were subsequently incubated with a baculovirus expressed, purified p52 form of Shc.3 After washing, the filters were incubated with anti-Shc. As shown in Fig. 5C, Shc association with GST-cadherin was detected only in those samples where GST-cadherin had been previously incubated with c-Src and ATP. This demonstrates a direct interaction between Shc and the tyrosine phosphorylated cytoplasmic domain of cadherin. As shown in Fig. 5D, this direct interaction was also observed when isolated p52 Shc was added to Sepharose beads coupled to GST or GST-cadherin, which had been preincubated with c-Src and/or ATP. Following washing of the beads, elution with glutathione, and SDS-PAGE, Western blotting showed that Shc associated only with tyrosine phosphorylated GST-cadherin.
Physiological requirements for cell proliferation, particularly within
tissues, include the coordinated modulation of intracellular functions,
such as nuclear transcription and cytoskeletal structure, with changes
in the relationship of cells to their immediate extracellular environment, such as neighboring cells and the extracellular matrix. Cadherins represent a major molecular system by which cell-cell adhesion occurs. The results described in this manuscript demonstrate a
Shc-cadherin association that is modulated by extracellular Ca2+ and EGF. This raises the possibility that Shc, a
tyrosine kinase substrate, may participate in the control of cadherin
function in addition to its known role in the mitogenic activation of
Ras and thereby nuclear signaling. Interaction of cells with the
extracellular matrix is mediated by receptors termed integrins.
Recently, Shc association with the tyrosine phosphorylated
4 integrin subunit has been reported (28). The
4-Shc complex has been shown to be dissociated by EGF
treatment of cells (29) and the loss of Shc association capacity by
integrins results in aberrant cell cycle progression (30). Hence Shc
may function to coordinate multiple alterations in cell physiology
necessary for proliferation.
We thank Drs. Stan Hollenberg, Kathleen Keegan, and Steve Hanks for providing reagents for the yeast two-hybrid system. Dr. Benjamin Margolis is acknowledged for the generous gift of p52 Shc cDNA. We also thank Lan Qian, Feng-Lei Sun, and Sandra Ermini for DNA sequencing and cell culture assistance.