Reduction of trans-4,5-Dihydroxy-1,2-dithiane by Cellular Oxidoreductases Activates gadd153/chop and grp78 Transcription and Induces Cellular Tolerance in Kidney Epithelial Cells*

(Received for publication, April 7, 1997)

Margaretann M. Halleck Dagger , Hong Liu , Jason North and James L. Stevens §

From the W. Alton Jones Cell Science Center, Lake Placid, New York 12946

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES


ABSTRACT

trans-4,5-Dihydroxy-1,2-dithiane, the intramolecular disulfide form of dithiothreitol (DTTox) transcriptionally activates the stress-responsive genes gadd153(chop) and grp78. Herein, we used a renal epithelial cell line, LLC-PK1, to investigate the mechanism(s) whereby DTTox activates a molecular stress response. DTTox activated both grp78 and gadd153 transcriptionally, but gadd153 mRNA stability also increased suggesting that both transcriptional and posttranscriptional mechanisms are involved. DTTox did not activate hsp70 transcription indicating that a heat shock response was not induced. Structure-activity studies showed that DTTox analogues lacking the intramolecular disulfide were inactive. Furthermore, the ring-open intermolecular disulfide form of DTTox, 2-hydroxyethyl disulfide, was only a weak inducer of grp78 and gadd153 but was a strong inducer of hsp70 mRNA and a potent oxidant that lowered the NADPH/NADP+ ratio and depleted reduced glutathione (GSH). DTTox had little effect on the overall GSH and NADPH levels; thus cells were not undergoing oxidative stress; however, the NADPH/NADP+ ratio decreased slightly indicating that reducing equivalents were consumed. LLC-PK1 cells reduced DTTox to DTT, and the kinetics as well as the concentration dependence for reduction correlated with induction of both grp78 and gadd153 mRNA. Prior treatment with DTTox rendered cells tolerant to the potent nephrotoxicant S-(1,1,2,2-tetrafluoroethyl)-L-cysteine. Bacitracin, an inhibitor of plasma membrane oxidoreductases, blocked DTTox reduction and gene activation as well as DTTox-induced tolerance. Thus, activation of stress genes and induction of cellular tolerance by DTTox is mediated by a novel mechanism involving cellular oxidoreductases.


INTRODUCTION

Mounting evidence indicates that perturbation of the cellular thiol-disulfide redox potential activates gene expression. For example, depletion of cellular GSH causes protein thiol oxidation and activation of hsp70 transcription (1, 2). Likewise, perturbing cellular Ca2+ with ionophores or toxicants that cause oxidative stress activates c-fos, c-myc, and the growth arrest and DNA damage-inducible gene, gadd153 (3-7). In some cases, oxidants may directly regulate transcription factors, such as oxyR, by protein thiol oxidation (8). On the other hand, oxidation of a cysteinyl residue in Fos or Jun blocks DNA binding (9). Other transcription factors including NFkappa B, p53, Egr-1, Usf, and Srf, to name a few, are also regulated directly or indirectly by oxidation (10-14).

Cellular sensors capable of transducing redox signals to the nucleus include protein phosphatases, protein kinases, hormone-responsive calcium pools, and cellular glutathione pools, all of which respond to oxidant exposure (15-18). In particular, oxidation or reduction of cellular protein and nonprotein thiols may be an important link between toxicant exposure and altered gene expression (1, 2). Although oxidative stress has been studied more extensively, reductive stress also activates stress-response genes. For example, organic thiols increase expression of the glucose-regulated protein grp78 and gadd153 genes (19-22). gadd153, also called chop for C/EBP homologous protein, is a member of the C/EBP gene family of transcription factors (23, 24) and is particularly interesting because, unlike grp78 (20), it is activated by either oxidative or reductive stress (22, 25, 26).

The LLC-PK1 cell line, a porcine renal epithelial cell line, retains many characteristics of the proximal tubule epithelium (27, 28) and has been used to investigate mechanisms of oxidative and reductive toxicity as well as stress-gene activation in the kidney (1, 6, 21, 22, 29-33). Dithiothreitol (DTT),1 a powerful disulfide reducing agent, is toxic to LLC-PK1 cells and activates both grp78 and gadd153 (20, 22). In preliminary structure-activity studies we found that the nontoxic intramolecular disulfide form of DTT, trans-4,5-dihydroxy-1,2-dithiane (DTTox), also induced gadd153 and grp78 mRNA. Since both genes are activated by thiol-induced reductive stress (20, 22), it was surprising that DTTox, an intramolecular disulfide with a very high reduction potential, was a good inducer. Herein, we show that reduction of DTTox by cellular oxidoreductases mediates transcriptional activation of gadd153 and grp78 as well as induction of cellular tolerance to chemical toxicants. In a related study, we show that induction of cellular tolerance by DTTox depends on its ability to activate an endoplasmic reticulum stress response and to increase expression of chaperone proteins located in the endoplasmic reticulum (34). Thus, DTTox is a novel activator of stress-gene expression and will be useful in probing the role of the thiol/disulfide redox status in the cellular and molecular responses to stress.


EXPERIMENTAL PROCEDURES

Cell Culture and Experimental Treatment

LLC-PK1 cells (27) were obtained from American Tissue Type Culture (Rockville, MD) and were used between passages 205 and 215. Cells were grown to confluence in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Life Technologies, Inc. or Upstate Biotechnology, Lake Placid, NY) in an atmosphere of 5% CO2, 95% air in a humidified 37 °C incubator. Cells (1.25 × 106) were plated in 100-mm dishes 5 days prior to experimental treatment. The medium was routinely changed 3 days after seeding. Confluent cultures of LLC-PK1 cells were rinsed with phosphate-buffered saline and treated with DTTox or TFEC prepared fresh in Earle's balanced salt solution (1.8 mM CaCl2, 5.4 mM KCl, 1.7 mM MgSO4, 26.2 mM NaHCO3, 1.0 mM NaH2PO4, 5.6 mM glucose, and 25 mM HEPES). Actinomycin D was dissolved in absolute ethanol at a concentration of 5.0 mg/ml, and 1 µl of the ethanol stock solution was added to 1 ml of Earle's balanced salt solution to achieve a final working concentration of 5 µg/ml.

Northern Analysis

Total RNA was isolated from cells using RNAzol supplied by Cinna/Biotecx (Houston, TX). Poly(A)+ RNA was isolated using oligo(dT)-cellulose, a standard technique. Northern analysis was performed on blots on which either total RNA (40 µg) or poly(A)+ RNA (5 µg) had been size separated by electrophoresis in 1.4% agarose denaturing gels. After prehybridization, blots were hybridized (21) overnight with cDNA probes for gadd153 (35) or grp78 (36), a gift of Dr. Amy Lee, and labeled with [alpha -32P]dCTP by random priming (37) using a kit (Boehringer Mannheim). Blots were allowed to decay before reprobing with a 200-base pair cDNA insert for mouse beta -actin cDNA excised from pMACTE4, an SK-based plasmid (a gift of Dr. D. Eisinger). Bands were visualized by autoradiography using DuPont Cronex film and an intensifying screen and were quantitated using a BioImage Densitometer (Ann Arbor, MI). To correct for differences in gel loading, integrated optical densities were normalized to beta -actin. Since gadd153 mRNA is barely detectable in untreated cells, the optical density values determined for gadd153 and grp78 expression after 10 mM DTTox or 10 mM DTT treatment were arbitrarily designated as the 100% response, and all other values were expressed as a percent of that value.

Nuclear Run-on Analysis

Following treatment, cells were lysed in 0.5% Nonidet P-40, and nuclei were stored at -70 °C in 40% glycerol (38). Newly transcribed RNA, labeled with [alpha -32P]UTP and purified by the method of Celano et al. (39), was hybridized to linearized plasmids that had been UV cross-linked to nylon filters. Plasmids containing cDNAs for beta -actin and hsp70 (plasmid pAT125, American Type Culture Collection) served as internal controls, and pBluescript SK (Stratagene, La Jolla, CA) was used to assess nonspecific binding. Run-on transcription was quantitated using a BioImage Densitometer. The run-on data presented in the results are a summary of three separate experiments but were not normalized to beta -actin since DTTox and cycloheximide both perturbed beta -actin transcription somewhat.

Biochemical Assays

Protein and nonprotein thiols were determined spectrophotometrically using Ellman's reagent (5,5'-dithiobis(2-nitrobenzoic acid)) (40) as described previously (41). Intracellular GSH was measured by the high performance liquid chromatography method of Reed et al. (42). Nonprotein thiol values measured with Ellman's reagent were very similar to GSH values measured directly by high performance liquid chromatography analysis. Pyridine nucleotides were determined using the enzymatic cycling assay of Passonneau and Lowry (43). Protein was determined using the Bio-Rad protein assay with bovine IgG as the standard. DTTox reduction to DTT was determined by measuring the nonprotein thiols in the medium.

Cytotoxicity Assays

Cytotoxicity was assessed by measuring release of lactate dehydrogenase into the medium as described (44). Unlabeled or 35S-labeled S-(1,1,2,2-tetrafluoroethyl)-L-cysteine (TFEC) were synthesized as before (45). When covalent binding of reactive TFEC metabolites was determined as an indication of uptake and activation, cells were treated for 2 h with [35S]TFEC, and then binding of 35S-metabolites was determined as described (44).

Statistics Analysis

Analysis of variance followed by the Scheffe F test for multiple comparisons was used to compare means of three or more groups. The level of significance was set at p < 0.05.


RESULTS

Characterization of DTTox Induction of grp78 and gadd153 mRNA

Previous work in our laboratory demonstrated that DTT treatment induced both grp78 and gadd153 mRNA in LLC-PK1 cells (21, 22). Since DTT and DTTox differ only in their thiol oxidation state, we first characterized the induction of gadd153 and grp78 mRNA by DTTox and then compared the data to induction by DTT (Fig. 1). DTTox induced both gadd153 and grp78 mRNA expression in a time- and concentration-dependent manner. At 10 mM, DTT and DTTox were equally effective inducers of gadd153 and grp78 mRNA. However, DTT was effective at lower concentrations and at earlier times compared with DTTox. We also tested analogues of DTTox (Fig. 2). trans-1,2-Cyclohexanediol lacks the intramolecular disulfide bond and was ineffective. 2-Hydroxyethyl disulfide, the ring-open intermolecular disulfide analogue of DTTox, proved to be extremely toxic but induced grp78 and gadd153 only modestly. However, 2-hydroxyethyl disulfide was a potent inducer of hsp70 because of its ability to deplete cellular glutathione (see below), a known stimulus for hsp70 transcription (1, 2). Thus, the intramolecular disulfide was a necessary functional group for activation of grp78 and gadd153 by DTTox.


Fig. 1. Comparison of grp78 and gadd153 induction by DTTox and DTT. LLC-PK1 cells were treated with DTTox or DTT at various concentrations for 5 h (A and C) or with 10 mM DTTox or DTT for periods up to 5 h (B and D). Following treatment, poly(A)+ RNA was prepared for Northern blot analysis with either grp78 (A and B) or gadd153 (C and D). The inset in A shows a representative blot from a single experiment in which cells were either treated with Earle's balanced salt solution alone (lane 1) or were induced by treatment with 10 mM DTT (lane 2) or DTTox (lane 3). Blots from two experiments were quantitated by scanning densitometry, and the integrated optical densities were converted to a relative RNA value expressed as a percent relative to the response observed with 10 mM inducer (A and C) or after a 5-h treatment (B and D). The data are the average ± the range of two separate experiments (n = 2).
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Fig. 2. Structure of DTTox analogues. Each analogue was tested as an inducer of grp78, gadd153, or hsp70 mRNA. Cells were treated with the indicated concentrations of inducer and poly(A)+ or total RNA prepared for Northern blot analysis. The results of Northern analysis from at least three independent experiments (n = 3) are summarized for each compound with regard to the potency as an inducer of grp78, gadd153, or hsp70. A strong inducer indicates that there was at least a 10-fold induction in each experiment, whereas a weak inducer caused only a 2-5-fold increase. Induction of less than 2-fold was deemed no induction (none). The qualitative summary is derived from data collected in three separate experiments (n = 3).
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Transcriptional and Posttranscriptional Mechanisms of grp78 and gadd153 mRNA Induction

To determine if DTTox treatment transcriptionally activated gadd153 and grp78 in LLC-PK1 cells, nuclear run-on analyses were performed (Fig. 3). Since hsp70 is more responsive to oxidative than reductive stress (20) and is not activated by DTT (22, 29), we also analyzed hsp70 transcription. Basal grp78 transcription was much higher than gadd153; however, treatment with 10 mM DTTox transcriptionally activated grp78 and gadd153 16 ± 9- and 9 ± 4-fold, respectively (n = 3), but failed to activate hsp70 transcription. DTT also increased transcription of gadd153 and grp78 markedly, 13 ± 6- and 18 ± 14-fold, respectively (n = 3). There was also a significant and reproducible decrease in beta -actin transcription in cells treated with DTTox, but the mechanism underlying this effect is not clear at present.


Fig. 3. Nuclear run-on analysis of grp78 and gadd153 transcription. RNA newly transcribed in isolated nuclei in the presence of [alpha -32P]UTP was extracted and hybridized with cDNA inserts immobilized on nylon membranes. Nuclei were prepared from cells treated for 2.5 h with Earle's balanced salt solution alone (Control) or with 10 mM DTT or DTTox. The + designation indicates that cells were treated in the presence of cycloheximide (CXM, 50 µg/ml) to determine the dependence of transcription on protein synthesis. The data are from a single experiment representative of three (n = 3). Means with standard deviations for induction are reported in the text.
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Transcriptional activation can be classified as a primary or secondary response based on cycloheximide sensitivity (46). Cycloheximide treatment caused only a modest inhibition of DTT-mediated induction of gadd153 and grp78 mRNA (Fig. 4). In contrast, cycloheximide completely blocked induction of gadd153 and grp78 (94% inhibition) by DTTox. Similar results were obtained by nuclear run-on analysis; cycloheximide blocked DTT-mediated transcriptional activation of gadd153 and grp78 by 58 and 21%, respectively, whereas transcriptional activation induced by DTTox was completely blocked by cycloheximide for both genes (Fig. 3). These results indicate that transcriptional activation of grp78 and gadd153 by DTTox is dependent on protein synthesis.


Fig. 4. Effect of cycloheximide on induction of gadd153 and grp78 mRNA by DTTox. LLC-PK1 cells were treated with 10 mM DTTox or DTT in the presence and absence of cycloheximide (CXM, 50 µg/ml) and poly(A)+ RNA prepared for Northern analysis to assess gadd153 (A) and grp78 (B) relative mRNA expression. The data are the average ± the range of two separate experiments (n = 2).
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Although DTTox induction of gadd153 and grp78 mRNA appeared to be due in part to transcriptional activation, run-on experiments do not exclude effects on posttranscriptional processes (47). Therefore, we used actinomycin D, an inhibitor of transcription, to examine the contribution of mRNA stability to grp78 or gadd153 mRNA accumulation. Actinomycin D blocked the induction of both mRNAs by DTT (Fig. 5) or DTTox (data not shown). When we first induced grp78 with DTT and then added actinomycin D to prevent further transcription, there was no observable decrease in grp78 mRNA in the presence of actinomycin D alone or actinomycin plus DTTox (data not shown), indicating that grp78 mRNA was extremely stable (t1/2 >18 h). However, gadd153 mRNA was very unstable (t1/2 = 2 h) in the presence of actinomycin D (Fig. 5); DTTox dramatically increased gadd153 mRNA stability (t1/2 >18). Thus, posttranscriptional processes contribute to the increase in gadd153 but not grp78 mRNA after DTTox treatment.


Fig. 5. Effect of DTTox on the stability of gadd153 mRNA. Bottom panel, a representative Northern analysis of gadd153 mRNA expression with and without actinomycin D (ActD) present during the induction or chase period. The conditions for actinomycin D chasing are shown along with the beta -actin control from the same blot. To determine the effect of DTTox on gadd153 and grp78 mRNA stability, cells were first treated with Earle's balanced salt solution alone (control lane) or with 10 mM DTT for 2 h (lane labeled DTT) to induce gadd153 and grp78. When actinomycin D was added along with DTT during the 2-h induction period (lane labeled DTT+ActD), there was no increase in mRNA for either gene (grp78 data not shown). Grp78 mRNA was very stable in the presence of actinomycin D alone whether or not DTTox was present (data not shown; see text). When cells were chased in actinomycin D after the 2-h DTT induction, cells were rinsed, and then the medium was replaced with Earle's containing 5.0 µg/ml actinomycin D, to prevent further transcription during the chase period, in the presence (ActD + DTTox) or absence (ActD) of DTTox. The chasing time (from 0.5 through 6 h) is indicated by the numbers below the lanes on the right side of the lower panel. In all cases, poly(A)+ RNA was prepared for Northern analysis using grp78 and gadd153 cDNA probes. Top panel, semi-log plot showing the decay rate of gadd153 mRNA in the presence of actinomycin D (Act D) alone or actinomycin D plus DTTox (Act D/DTTox). Values were determined by densitometry and were normalized to beta -actin. The data are from a single experiment representative of two separate experiments (n = 2). The t1/2 values for grp78 and gadd153 mRNA are reported in the text.
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Induction of grp78 and gadd153 by DTTox Correlates with Reduction

Exogenous disulfides are reduced by GSH- and/or NAD(P)H-dependent cellular reductase systems (48). As a consequence, disulfides can induce oxidative stress by depleting cellular GSH and NAD(P)H. Although the redox potential of DTTox does not favor reduction (49), LLC-PK1 cells reduced DTTox in a time- and concentration-dependent fashion (Figs. 6 and 7). The concentration dependence for DTTox reduction correlated with induction of grp78 and gadd153 mRNA (Fig. 7). However, DTTox treatment did not alter the levels of GSH or protein thiols (Table I). The only significant change was in the nicotinamide adenine dinucleotide phosphate pool where a modest, yet significant, decrease in the NADPH/NADP+ ratio indicated that reducing equivalents were consumed by DTTox reduction (Table I). Although 2-hydroxyethyldisulfide was a poor inducer of grp78 and gadd153, it was reduced to a much greater extent than DTTox (666 ± 155 versus 174 ± 66 nmol/h for 10 mM 2-hydroxyethyldisulfide and DTTox, respectively). Accordingly, the NADPH/NADP+ ratio dropped dramatically after 2-hydroxyethyldisulfide treatment as did GSH (Table I), consistent with a severe oxidative stress. Severe GSH depletion activates the heat shock response (1, 2) and 2-hydroxyethyl disulfide was a strong inducer of hsp70 transcription (Fig. 2).


Fig. 6. Time-dependent reduction of DTTox by LLC-PK1 cells. Earle's balanced salt solution containing 10 mM DTTox was added to confluent cultures of LLC-PK1 cells, and aliquots of the medium were removed at various times thereafter for analysis of nonprotein thiols (NPSH) as a measure of DTTox reduction. The data show the result from triplicate wells taken from one experiment representative of three.
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Fig. 7. Comparison of the concentration dependence for DTTox reduction and induction of grp78 and gadd153 mRNA by LLC-PK1 cells. The concentration dependence for reduction of DTTox in LLC-PK1 cells and expression of gadd153 or grp78 mRNA is shown. Cells were treated with various concentrations of DTTox for 5 h at which time the nonprotein thiol (NPSH) concentrations in the medium were measured, as a measure of DTTox reduction, and poly(A)+ RNA was prepared for Northern analysis. The relative mRNA refers to the percent of the maximal response achieved with 10 mM DTTox treatment. The data are the average ± the range of two separate experiments (n = 2).
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Table I. Effect of DTTox and 2-HED on cellular NADPH and GSH

LLC-PK1 cells were grown in 100-mm dishes and were treated with either Earle's balanced salt solution alone or with Earle's containing 10 mM DTTox or 10 mM 2-hydryoxyethyl disulfide (2-HED) in a 10-ml volume. After 5 h the total nicotinamide adenine dinucleotide phosphate pool (Total) and the individual pools of NADPH and NADP+, in pmol/mg cellular protein, and the ratio were calculated for each experiment. Cellular GSH was determined by HPLC, and protein thiols (PSH) were determined using Ellman's reagent (see "Experimental Procedures") and are expressed as nmol of DTNB reactive substance per 100-mm dish. Significant differences were determined by analysis of variance. The data are the mean ± S.D. of data from 6 (n = 6) and 3 (n = 3) separate experiments for the controls and treatment groups, respectively.

Control DTTox 2-HED

Total (pmol/mg) 388  ± 46 414  ± 36 605  ± 127a
NADPH 373  ± 49 366  ± 49 367  ± 83
NADP+ 23  ± 5 37  ± 6 174  ± 42a
NADPH/NADP+ 17  ± 4 10  ± 1a 2  ± 1a
GSH (nmol) 30  ± 7 29  ± 3 11  ± 2a
PSH (nmol) 256  ± 84 262  ± 79 209  ± 30

a Means that were different from other treatment or control means.

Bacitracin inhibits disulfide reduction by oxidoreductases at the plasma membrane (50) and inhibited DTTox reduction by LLC-PK1 cells almost completely (Fig. 8). Bacitracin also blocked 2-hydroxyethyl disulfide reduction (data not shown). To determine if reduction of DTTox was linked to gadd153 and grp78 expression, LLC-PK1 cells were treated with DTTox in the presence and absence of bacitracin, and Northern blot analyses were performed. As shown in Fig. 9 bacitracin attenuated the induction of gadd153 and grp78 mRNA in a dose-dependent manner.


Fig. 8. Concentration dependence for bacitracin inhibition of DTTox reduction by LLC-PK1 cells. LLC-PK1 cells were incubated for 5 h with various concentrations of bacitracin in the presence or absence of 10 mM DTTox. After 5 h, an aliquot of the medium was taken to determine the amount of nonprotein thiols (NPSH) present. Bacitracin itself reacted with Ellman's reagent weakly accounting for the slight rise in the base-line media nonprotein thiol concentration in the absence of DTTox. The entire concentration range was performed in only one experiment; however, the data at 10 mM bacitracin are representative of five separate experiments (n = 5).
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Fig. 9. Effect of bacitracin on grp78 and gadd153 mRNA induction. LLC-PK1 cells were treated for 5 h with DTTox (10 mM) in the presence or absence of bacitracin (3 or 10 mM). Total RNA was prepared and Northern analysis of grp78 and gadd153 mRNA expression performed on a 40-µg aliquot. The data shown in the Northern blot were quantitated by scanning densitometry, and the results are shown as induction relative to control (no treatment) at the right. The data are from a single experiment representative of three (n = 3).
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DTTox Treatment Causes Cytoprotection

Although DTTox did not activate a heat shock response, induction of other stress-response genes, including grp78, has been shown to protect cells against subsequent toxicant treatment (34, 51-54). Therefore we determined if DTTox treatment rendered cells tolerant to subsequent toxic insults. TFEC is a potent nephrotoxicant that kills renal epithelial cells after activation to a reactive acylating species (55). When cells were treated with TFEC immediately after DTTox treatment, there was no protection (Fig. 10). However, when cells were allowed to recover for 6 h, tolerance developed despite the fact that the cells were still able to activate [35S]TFEC to reactive species which covalently bound to cellular macromolecules indicating that there was no change in the uptake or metabolism of [35S]TFEC (Fig. 10, inset). Tolerance was maintained up to 24 h after DTTox removal. Simultaneous treatment with DTTox and bacitracin blocked induction of tolerance to TFEC even though bacitracin was removed during the recovery period (Fig. 11). Therefore, DTTox induces cellular tolerance in a manner consistent with activation of gene expression and by a signaling pathway linked to cellular oxidoreductase activity. DTTox itself at a concentration of 10 mM did not produce any increase in lactate dehydrogenase release compared with LLC-PK1 cells treated with EBSS alone under any conditions tested. In addition, DTTox treatment did not cause collapse of the domes (data not shown), an indication of active transport, under the conditions used here (22).


Fig. 10. DTTox treatment prevents TFEC-induced cell death. Naïve cells (pretreated with balanced salt solution alone) or cells that had been pretreated with DTTox (10 mM) for 3 h were either treated immediately with TFEC (0.5 mM for 3 h) in a balanced salt solution or were returned to Dulbecco's modified Eagle's medium containing 10% fetal bovine serum and allowed to recover for various periods (6, 12, or 24 h). The time on the abscissa indicates the amount of time the cells were allowed to recover before TFEC was added. After allowing the cells to recover, they were challenged with TFEC (0.5 mM for 3 h) in a balanced salt solution and returned to complete medium. Cell death was measured as release of lactate dehydrogenase (%LDH Release) 3 h after TFEC treatment in all cases. Covalent binding was determined as described (44) after treating cells with [35S]TFEC (0.5 mM) for 2 h. Covalent binding of [35S]TFEC metabolites is shown in the inset and is reported as nmol bound/mg of cell protein. The lactate dehydrogenase data are the mean ± S.D. from triplicate wells collected in a single experiment and are representative of four separate experiments. The binding data are the mean ± S.D. from three separate experiments.
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Fig. 11. Bacitracin inhibits DTTox induction of cellular tolerance. Cells were treated with DTTox as in Fig. 10 in the presence (DTTox/BAC) or absence of 10 mM bacitracin. As a control, cells were also pretreated with bacitracin alone (BAC). After the 3-h induction period, both bacitracin and DTTox were removed, and the cells were allowed to recover for 6 h and then challenged with TFEC (0.5 mM for 3 h). Lactate dehydrogenase release (% LDH Release) was determined as described in the legend to Fig. 10. Means that were not significantly different (p < 0.05) are denoted by a common letter designation. Means with different letter designation are significantly different (p < 0.05) as determined by analysis of variance.
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DTT itself activates grp78 and gadd153 in LLC-PK1 cells (22). To determine if the ability of DTTox to activate gene expression and induce tolerance depended merely on the DTT produced by reduction of the oxidized form, we carried out two experiments. First, we added DTTox to the cells and then washed the cells and applied fresh DTTox every 30 min throughout the induction period (repeated addition protocol). In this way, DTT was never allowed to accumulate over a concentration of 25-50 µM (see Fig. 6). Using LDH release as an index of tolerance, the repeated addition protocol for DTTox treatment produced a reduction in LDH release, from 63% in unconditioned cells to 29% in DTTox-pretreated cells (average of two experiments) compared with 31% LDH release when DTTox was left in for the full 3-h pretreatment period. Second, we added DTT at concentrations of 10, 25, 50, and 100 µM to the culture medium for the full 3-h induction period. DTT treatment under these conditions did not induce tolerance to TFEC (data not shown). Finally, we showed that adding DTT at the above concentrations in the presence of 10 mM DTTox did not increase or decrease the development of tolerance (data not shown). Thus, it would appear that the induction of tolerance is not due simply to the reduction product of DTTox, i.e. DTT.


DISCUSSION

Cells maintain a reducing thiol-disulfide redox potential in the cytoplasm and an oxidizing potential in the endoplasmic reticulum (56). Perturbation of the thiol-disulfide redox potential in either compartment activates stress-response genes (1, 2, 19, 56). The molecular response to DTTox resembles the reductive stress response induced by DTT and was differentiated from the response to the ring-open intermolecular disulfide analogue 2-hydroxyethyl disulfide, a strong oxidant and activator of hsp70. DTTox also induced tolerance to the nephrotoxicant TFEC but did not activate hsp70 transcription consistent with the observation that prior heat shock does not protect against toxicant damage in LLC-PK1 cells (34). Bacitracin, an oxidoreductase antagonist, blocked disulfide reduction, inhibited gene activation, and prevented development of tolerance. Thus, DTTox is a novel inducer of stress-response genes and cellular tolerance but utilizes an hsp70-independent mechanism that requires reduction of the intramolecular disulfide by cellular oxidoreductases. Plasma membrane oxidoreductases that link the extracellular and intracellular redox environments may be involved (50).

We should point out that the bacitracin effect could be due in part to the fact that it inhibited [3H]leucine incorporation in LLC-PK1 cells (data not shown) since cycloheximide also blocked induction of gadd153 and grp78. However, several factors suggest this is not the case. First, the effect of bacitracin cannot be attributed solely to inhibition of protein synthesis since it was less effective in preventing grp78 induction compared with gadd153, yet both genes were equally sensitive to cycloheximide. Second, induction of grp78 and gadd153 by DTTox occurred even in a balanced salt solution which is already devoid of amino acids. Third, addition of bacitracin prevented reduction of DTTox and cellular tolerance even after the two compounds were removed and the cells returned to complete medium for 6-12 h. Thus, when taken together, the dose response, time course, structure-activity, and bacitracin inhibition data indicate that reduction of DTTox by cellular oxidoreductases is a prerequisite for gene activation.

The efficacy of DTTox in a particular cell type will depend on the presence of appropriate oxidoreductases since nonenzymatic reduction of DTTox is not favored (49). However, the nature of the oxidoreductase that mediates gene activation in LLC-PK1 cells is not clear. Either protein disulfide isomerase- or thioredoxin-like enzymes might reduce DTTox using GSH and/or nicotinamide adenine dinucleotide pools as reducing equivalents (48, 57, 58). DTTox was not reduced in cell lysates supplemented with NADPH alone (data not shown). Thus, an oxidoreductase system that utilizes the reducing potential of both GSH and NADPH may reduce DTTox, but further work will be necessary to determine the nature of this oxidoreductase activity. Since bacitracin inhibits exofacial redox activity at the plasma membrane (50), some DTTox reduction may take place outside the cell. Such an enzyme system may also serve as a redox-sensitive sensor between the extracellular and intracellular environments.

Either the mild stress on the NADPH/NADP+ ratio caused by DTTox reduction or the presence of DTT, the reduction product, could play a role in DTTox activation of gene expression. DTT is a potent reducing agent that inhibits intramolecular disulfide formation in the endoplasmic reticulum causing unfolded polypeptides to accumulate (59, 60). Agents that block protein processing in the endoplasmic reticulum activate both grp78 and gadd153 expression (61-63). On that note, it is possible that cycloheximide prevents grp78 and gadd153 activation because protein synthesis is inhibited, thus preventing nascent polypeptide chains from accumulating in the endoplasmic reticulum. However, the ability of DTTox to induce grp78 and gadd153 is not simply due to the reduction to DTT since DTT at the concentrations expected from reduction of DTTox were not effective in inducing tolerance. It seems that the ability of DTTox to induce grp78 and gadd153 depends on a complex combination of the enzymatic reduction coupled to use of cellular reducing equivalent in addition to the production of DTT.

DTT and other classic endoplasmic reticulum stress inducers not only disrupt protein processing but also inhibit protein synthesis generally and can be quite toxic (64, 65). Unlike DTT, DTTox does not inhibit protein synthesis, did not induce dome collapse in LLC-PK1 cells, as does DTT (22), nor did it cause lactate dehydrogenase release from LLC-PK1 cells, yet paradoxically, it activated grp78 expression. It seems likely that the answer to this apparent paradox lies in the fact that cells compensate for the mild stress caused by DTTox treatment by increasing expression of chaperones, like grp78, thus maintaining endoplasmic reticulum protein processing. When DTTox is removed, the cell is left with a high level of chaperones in the endoplasmic reticulum and cell injury is prevented. [35S]Methionine metabolic labeling studies show that GRP78 and GRP94 synthesis occur during the first 4 h after DTTox removal, a time course that correlates with the induction of tolerance (34). In addition, expression of an antisense grp78 mRNA in LLC-PK1 cells blocked the ability of DTTox to produce a tolerant phenotype to another toxicant, iodoacetamide (34). Therefore, the ability of DTTox to induce tolerance depends on induction of grp78 expression. Induction of gadd153 by endoplasmic reticulum stress has been reported by others (22, 62, 63). However, it seems unlikely that gadd153 plays a role in tolerance given that ectopic expression of gadd153 induces apoptosis in myeloblastic leukemia cells (66).

In conclusion, DTTox is a novel pharmacological tool to investigate the relationship between stress protein induction and cell death. Since DTTox is a potent radioprotective agent in mice in vivo under conditions that cause no apparent adverse effect to the animals (67), its utility may extend to whole animals. Moreover, a novel mechanism that links cellular oxidoreductases with the signaling pathways which activate transcription of two stress-responsive genes, grp78 and gadd153, has been elucidated. Given that redox regulation of gene expression is an important physiological mediator of genomic stress responses, our data suggest that cellular oxidoreductase activity may be an important determinant of cellular responsiveness. When taken in context with our observations that induction of tolerance by DTTox depends on grp78 induction (34), the data suggest that DTTox may be a very useful tool to study the physiological role of endoplasmic reticulum stress in cellular tolerance in vivo and in vitro.


FOOTNOTES

*   These studies were supported by U. S. Public Health Service Grants DK46267 and DK38965 (to J. L. S.) and National Research Services Award ES05569 (to M. M. H.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Dagger    Present address: Schering-Plough Research Institute, P. O. Box 32, Lafayette, NJ 07848.
§   To whom correspondence should be addressed: W. Alton Jones Cell Science Center, 10 Old Barn Rd., Lake Placid, NY 12946. Tel.: 518-523-1253; Fax: 518-523-1849; E-mail: jstevens{at}northnet.org.
1   The abbreviations used are: DTT, dithiothreitol; DTTox, oxidized dithiothreitol; TFEC, S-(1,1,2,2-tetrafluoroethyl)-L-cysteine.

ACKNOWLEDGEMENTS

We thank Dr. Amy Lee for providing the grp78 cDNA probe, for helpful discussions, and for sharing unpublished data. Critical reading of the manuscript by Dr. Nikki Holbrook was greatly appreciated.


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