(Received for publication, February 20, 1996, and in revised form, September 30, 1996)
From the Department of Biochemistry, University of Vermont, Burlington, Vermont 05405-0068
The effects of the stoichiometric inhibitors
tissue factor pathway inhibitor (TFPI), antithrombin-III (AT-III) and
heparin cofactor-II (HC-II) on thrombin generation were evaluated in a reaction system composed of coagulation factors VIIa, X, IX, VIII, and
V and prothrombin initiated by tissue factor (TF) and phospholipids. Initiation of the reaction in the absence of inhibitors resulted in
explosive thrombin generation for factor VIIa·TF concentrations varying from 100 to 0.25 pM with the lag time or initiation
phase of thrombin generation increasing from 0 to 180 s with
decreasing factor VIIa·TF concentrations. During the propagation
phase, prothrombin is quantitatively activated to 1.4 µM
-thrombin. At normal plasma concentration (2.5 nM)
full-length recombinant TFPI prolonged the initiation phase of thrombin
generation 2-fold, and the rate of thrombin generation in the
propagation phase of the reaction was 25-50% that of the uninhibited
reaction when the reaction was initiated with 1.25-20 pM
factor VIIa·TF. Inhibition of the reaction by TFPI is associated with
a delay in factor V activation. In the presence of TFPI no explosive
thrombin generation was observed when factor VIII was omitted from
reactions initiated by factor VIIa·TF concentrations
20
pM. This indicates that in the presence of TFPI the factor
IXa·factor VIIIa pathway becomes essential at low factor VIIa·TF
concentrations. In the reconstituted system, AT-III (3.4 µM) did not prolong the initiation phase of thrombin generation when the reaction was initiated with 1.25 pM
factor VIIa·TF, nor did AT-III delay factor V activation. The rate of thrombin formation in the presence of AT-III was reduced to 30% that
of the uninhibited reaction, and the
-thrombin formed was rapidly
inhibited subsequent to its generation. The addition of HC-II alone at
its physiological concentration (1.38 µM) to the procoagulant mixture did not alter the rate or extent of thrombin generation. Subsequently, the thrombin formed was slowly inhibited by
HC-II. The slow inactivation of thrombin by HC-II does not contribute
to thrombin inhibition in the presence of AT-III. In contrast, the
combination of physiological levels of AT-III and TFPI inhibited
explosive thrombin generation initiated by 1.25 pM factor
VIIa·TF completely. The absence of prothrombin consumption indicated
that the combination of TFPI and AT-III is able to prevent the
formation of prothrombinase activity at low factor
VIIa·TF concentrations. The data indicate that TFPI potentiates the
action of AT-III by decreasing the rate of formation and thus the
amount of catalyst formed in the reaction, enabling AT-III to
effectively scavenge the limited traces of factor IXa and factor Xa
formed in the presence of TFPI. The initiation of thrombin generation by increasing factor VIIa·TF concentrations in the presence of physiological concentrations of TFPI and AT-III showed dramatic changes
in the maximal rates of thrombin generation over small changes in
initiator concentration. These data demonstrate that significant
thrombin generation becomes a "threshold-limited" event with regard
to the initiating factor VIIa·TF concentration in the presence of
TFPI and AT-III.
The extrinsic pathway of blood coagulation involves the activation of multiple coagulation factors leading to thrombin generation. The procoagulant reaction starts with the binding of activated factor VII (factor VIIa) to its cofactor, tissue factor (TF).1 TF is an integral membrane protein that is exposed as a result of vessel wall injury or cytokine activation of endothelial cells or peripheral blood monocytes. The membrane-bound factor VIIa·TF enzyme complex activates the zymogens factor X and factor IX by limited proteolysis (1). Factor IXa combines with factor VIIIa on the membrane surface to form a second complex that activates factor X. Once activated, factor Xa associates with factor Va on a membrane surface to form prothrombinase, which converts prothrombin into thrombin. (For reviews on blood coagulation and membrane-dependent reactions in blood coagulation see, respectively, Refs. 2 and 3). The thrombin initially formed accelerates further thrombin generation by feedback activation of the procofactors factor V and factor VIII. Thrombin may also activate factor XI (4, 5), which, in turn, activates more factor IX. Deficiencies in factors VII, X, IX, V, or VIII or prothrombin are associated with abnormal bleeding. Factor XI-deficient individuals rarely suffer from spontaneous bleeding; however, homozygotes may require replacement therapy during significant surgical challenge. Thrombin also activates platelets, which secrete their granule contents and aggregate upon activation. In addition, thrombin cleaves fibrinogen to generate the fibrin network and activates the protransglutaminase factor XIII. The fibrin-platelet aggregate, stabilized by factor XIIIa-catalyzed cross-links, forms the hemostatic plug, which maintains the integrity of the circulatory system following vessel wall perforation.
In normal hemostasis, the procoagulant system is in balance with anticoagulant systems involved in the termination of the hemostatic reaction and the fibrinolytic system, which dissolves clots once they are formed. The anticoagulant systems consist of several stoichiometric protease inhibitors, the tissue factor pathway inhibitor (TFPI), antithrombin-III (AT-III), and heparin cofactor-II (HC-II), and the dynamic protein C pathway, which involves thrombin, activated protein C, protein S, and thrombomodulin.
TFPI is a reversible, active site-directed inhibitor of factor Xa, which regulates coagulation by inhibiting factor VIIa·TF in a factor Xa-dependent manner (for a review on TFPI see Ref. 6). The TFPI·factor Xa complex binds to the factor VIIa·TF complex, resulting in the formation of a TF·factor VIIa·TFPI·factor Xa quaternary complex (7). Although no human deficiencies have been reported, the in vivo relevance of TFPI is supported by experiments that showed the sensitization of rabbits to TF-triggered disseminated intravascular coagulation after immunodepletion of TFPI (8).
AT-III is a serine protease inhibitor whose importance in hemostasis is confirmed by the association of thrombosis with heterozygous AT-III deficiency (for reviews on AT-III see Refs. 9 and 10). AT-III inhibits thrombin, factor Xa, factor IXa, factor VIIa, factor XIa, and factor XIIa in vitro by forming covalent complexes in which the active site of the protease is trapped. The action of AT-III is potentiated by heparinoids. The rate of inhibition by AT-III and the potentiation of the inhibition reaction by heparinoids varies for each target protease (10). Factor Xa is protected from inactivation by AT-III when in a membrane-associated complex with factor Va (11, 12). In contrast, factor VIIa inactivation by AT-III is only significant when the protease is bound to TF (13). The inactivation of TF-bound factor VIIa by AT-III probably involves the opening of the active site of factor VIIa upon TF binding, allowing factor VIIa inactivation by the active site-directed AT-III. The low enzymatic activity of free factor VIIa provides a mechanism by which traces of factor VIIa may circulate in blood (14).
HC-II is a serine protease inhibitor circulating in blood plasma at a concentration of ~1.2 µM (15). Thrombin is the only procoagulant reported to be inhibited by HC-II. The inhibition of thrombin by HC-II is potentiated by heparin and by dermatan sulfate (for a review on HC-II see Ref. 16). A heterozygous deficiency of HC-II (activity levels < 60%) is found in approximately 1% of the healthy population and does not appear to be a risk factor for thrombosis. A homozygous deficient individual has not yet been identified (16).
We have described reconstituted empirical (17) and mathematical (18) models for the tissue factor pathway to thrombin using purified coagulation factors and computer simulations based upon the known kinetic constants for the reactions thought to be essential in the procoagulant scheme. The reaction could be divided into two phases, an "initiation phase," in which factor V and factor VIII were quantitatively cleaved and trivial amounts of factor Xa and factor IXa were produced, and a "propagation phase" in which prothrombin was quantitatively activated (17). As the concentration of initiator (factor VIIa·TF) was reduced, the initiation phase was prolonged while the rate of thrombin generation in the propagation phase varied by only 5-fold over a 1000-fold range of factor VIIa·TF concentration. The initiation phase was shortened when the reaction was initiated in the presence of factor Va while the propagation phase was dependent upon factor VIII and factor IX at factor VIIa·TF concentrations below 100 pM. The data obtained with the reconstituted empirical model using purified coagulation factors were reasonably approximated by the mathematical model. The data presented here extend the empirical tissue factor pathway to thrombin studies to include TFPI, AT-III, and HC-II.
Phosphatidylserine from bovine brain,
phosphatidylcholine from egg yolk and Hepes were purchased from Sigma.
D-Phenylalanyl-L-arginine chloromethyl ketone
(FPR-ck) and the biotinylated product were provided as gifts by Dr. R. Jenny (Haematologic Technologies Inc., Essex Junction, VT). Spectrozyme
TH and Spectrozyme Xa were purchased from American Diagnostica, Inc.
Q-Sepharose FF was obtained from Pharmacia Biotech Inc. All other
reagents were of analytical grade. Mouse monoclonal antibodies
HFV-17,
HPC-2, and
HFX-10 were provided by Dr. William Church
(Thrombosis Center Antibody Core Facility, Department of Biochemistry,
University of Vermont).
In the experiments described, we mix together a potent mixture of
enzyme and cofactor precursors together. The prevention of spontaneous
activation requires extraordinary levels of purity. Contaminating
enzymes at femtomolar to picomolar levels cannot be tolerated. Human
coagulation factors X and IX and prothrombin were isolated from fresh
frozen plasma using the methods of Bajaj et al. (19). All
steps were performed at 4 °C. Factor X and factor IX were passed
over a polyclonal burro anti-human prethrombin-1 antibody
immunoaffinity column in 20 mM Tris, 150 mM
NaCl, 1 mM benzamidine pH 7.4 (TBS/benz) and concentrated
on a Q-Sepharose FF column. Traces of factor VII were removed by
washing the Q-Sepharose columns with 10 mM
CaCl2, 50 mM NaCl, 20 mM Tris, 1 mM benzamidine, pH 7.4. After the columns were washed with
buffer without CaCl2, factor X and factor IX were eluted
with TBS/Benz containing 1 M NaCl. Prothrombin was passed
over anti-factor X (HFX-10) and anti-protein C (
HPC-2)
immunoaffinity columns and was concentrated and depleted of trace
amounts of factor VII using Q-Sepharose as described for factor X and
factor IX. Prothrombin was eluted from the Q-Sepharose column with 20 mM CaCl2, 150 mM NaCl, 20 mM Tris, 1 mM benzamidine. To inhibit traces of
active enzymes in the zymogen preparations, the preparations of factor
X, factor IX, and prothrombin were treated with 1 mM
diisopropylphosphofluoridate at 25 °C for 1 h. The proteins
were then dialyzed against 20 mM Tris, 150 mM
NaCl, pH 7.4 (TBS) and treated with 10 µM FPR-ck for
1 h at 25 °C. Free FPR-ck and breakdown products were removed by extensive dialysis in TBS. The protein solutions were aliquoted and
frozen in liquid nitrogen and stored at
80 °C.
Human factor V was isolated by using the methods of Nesheim et al. (20). Recombinant factor VIII and recombinant tissue factor (residues 1-242) were provided as gifts by Dr. Shu Len Liu and Dr. R. Lundblad (Hyland Division, Baxter Healthcare Corp.). Recombinant human coagulation factor VIIa was purchased from NOVO pharmaceuticals. Recombinant full-length TFPI produced in Escherichia coli was provided as a gift by Dr. K. Johnson (Chiron Corp). AT-III and HC-II were purified from barium citrate absorbed plasma by heparin-Sepharose chromatography according to the method described by Griffith et al. (21). Possible traces of heparin contamination in the AT-III preparation were removed by DEAE-cellulose chromatography (21).
Coagulation Activation ExperimentsThrombin generation initiated by factor VIIa·TF in a fully reconstituted procoagulant model was studied as described previously (17). TF was relipidated at 0.5 nM into 400 µM 75% phosphatidylcholine, 25% phosphatidylserine vesicles (PCPS), for 30 min at 37 °C in 20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH 7.4 (Hepes/Ca2+). The relipidated TF was incubated with factor VIIa for 20 min at 37 °C to allow factor VIIa·TF complex formation. Factor V and factor VIII were added to the equilibrated factor VIIa·TF mixture (total volume of addition not exceeding 0.25% of the final reaction volume), and immediately thereafter the reaction was started by the addition of a solution containing factor X, factor IX, and prothrombin, equilibrated in Hepes/Ca2+ at 37 °C for 3 min before the addition. When TFPI, AT-III, or HC-II was included it was added to the factor X, factor IX, and prothrombin mixture.
The final concentrations of the proteins in the reaction, chosen to
represent mean plasma values, were 160 nM factor X, 90 nM factor IX, 0.7 nM factor VIII, 20 nM factor V, and 1.4 µM prothrombin (17). The
factor VIIa concentration was varied, while TF was kept constant and in
excess at 0.25 nM. The final PCPS concentration was 200 µM. Following initiation of the reaction, aliquots were withdrawn from the reaction mixture and quenched into either 20 mM EDTA/TBS, pH 7.4, to assay for thrombin formation or
into 2% SDS, 0.062 M Tris, 10% glycerol, 0.04%
bromphenol blue, pH 6.8, for SDS-PAGE and immunoblotting. Samples
quenched in SDS were heated for 5 min at 95 °C and stored at
20 °C. Assays for thrombin activity were performed using the
chromogenic substrate Spectrozyme TH. The hydrolysis of the substrate
was monitored by the change in absorbance at 405 nm using a Molecular
Devices Vmax spectrophotometer. Thrombin
generation was calculated from a standard curve prepared by serial
dilutions of
-thrombin. When AT-III or HC-II were added to the
reaction mixture, samples were withdrawn into 20 mM
EDTA/TBS containing 0.4 mM Spectrozyme TH and assayed
immediately for thrombin activity. Quenching the reaction mixture in
the resulting concentration of substrate stops the inactivation of
thrombin by AT-III during the assay. Thrombin activity in a mixture of
125 nM
-thrombin and 3.4 µM AT-III was
stable for 15 min in the EDTA/Spectrozyme TH mixture. Without
Spectrozyme TH,
-thrombin was completely inactivated by AT-III under
these conditions within 4 min. During the thrombin activity assay,
thrombin or AT-III concentrations did not exceed 70 nM or
170 nM, respectively. These concentrations are well below
those used in the control experiment, which showed the stability of
thrombin in a thrombin/AT-III/Spectrozyme TH mixture, thus assuring
accurate thrombin measurement in the presence of AT-III. Factor Xa
generation was measured in samples quenched in a mixture of 40 mM EDTA/TBS containing excess hirudin and 0.4 mM Spectrozyme Xa. This mixture prevents further inhibition
of factor Xa by AT-III as discussed above for thrombin. The hydrolysis of the substrate was measured as described for the thrombin assay. Prothrombinase concentrations were calculated from the rate
of thrombin generation using a kcat of 5016 min
1 (22).
In order to follow thrombin generation via active site blotting, samples were quenched into 100 µM biotinylated FPR-ck, in TBS containing 20 mM EDTA and incubated for 20 min at 25 °C. After incorporation of the active site label, samples were further quenched in 2% SDS, 0.062 M Tris, 10% glycerol, 0.02% bromphenol blue, pH 6.8, and heated for 5 min at 95 °C.
For active site and Western blot analysis, samples were subjected to
SDS-PAGE on 4-12% polyacrylamide gels under conditions described by
Laemmli (23). Following SDS-PAGE, the proteins were transferred to
nitrocellulose membranes for immunoblot or active site blot analysis
using techniques described by Towbin et al. (24). Membranes
were blocked for nonspecific binding with 5% nonfat dry milk in TBS
containing 0.05% Tween and probed for prothrombin activation products
using a polyclonal burro anti-human prethrombin-1 antibody (4 µg/ml)
(17) or probed for factor V and the factor Va heavy chain using a
murine anti-human factor V monoclonal antibody (HFV-17, 10 µg/ml).
The reactive bands were visualized with peroxidase-conjugated goat
anti-horse IgG or peroxidase-conjugated goat anti-mouse IgG (1:5000
dilution, Southern Biotechnology Associates, Inc.) using the
chemiluminescent substrate Luminol (Renaissance chemiluminescent
reagent, DuPont). Kodak X-Omat film was exposed to light emitted from
the hydrolysis of the added Luminol substrate. Active site blots were
blocked with 0.5% Tween in TBS and stained for incorporation of
biotinylated-FPR-ck using the Vectastain ABC kit (Vector Laboratories,
Inc.), which employs the multiple biotin binding sites on avidin to
link peroxidase-conjugated biotin to the active site-incorporated
biotinylated FPR-ck. Bound peroxidase was visualized using the
chemiluminescent technique as described above.
The effect of full-length TFPI on thrombin
generation initiated by the addition of factor VIIa·TF·PCPS to a
mixture of prothrombin and factors X, IX, V, and VIII was studied over
a wide range of initiator (factor VIIa·TF) concentrations. As
previously reported, in the absence of TFPI, the reaction profile can
be divided into two phases, an initiation or lag phase and a
propagation phase in which prothrombin is quantitatively cleaved to
thrombin (Fig. 1A). In the absence of TFPI
the rate of thrombin generation in the propagation phase increased only
5-fold when the initiator concentration was varied from 0.25 to 100 pM (Fig. 1A). However, increasing the factor
VIIa·TF concentration shortened the initiation phase of the reaction
from 180 s at 0.25 pM initiator to almost no lag with
100 pM factor VIIa·TF. The addition of 2.5 nM
TFPI (the approximate physiological concentration) resulted in
extension of the initiation phase (Fig. 1B) and a reduction
in the rate of thrombin generation during the propagation phase. A plot
of the duration of the lag time, estimated by the intersection of the
line through the linear part of the propagation phase and the
x axis, versus the factor VIIa·TF concentration
revealed that TFPI doubled the lag time, independent of the factor
VIIa·TF concentration (Fig. 2A). However,
explosive thrombin generation via ~100 pM prothrombinase still occurred during the propagation phase,
even at 0.25 pM factor VIIa·TF (Fig. 2B,
squares).
To investigate whether the effect of TFPI was a result of direct
inhibition of factor Xa or the inhibition of factor VIIa·TF activity,
an experiment was performed in which prothrombinase activity
was initiated by the addition of various factor Xa concentrations to a
mixture of 200 µM PCPS and factor IX, factor V, and
prothrombin (Fig. 3). The maximum concentration of
prothrombinase formed was calculated from the linear part of
the thrombin generation curve. TFPI (Fig. 3, open circles)
reduced prothrombinase activity by 50-65% when the
reaction was initiated with either 200 or 400 pM factor Xa.
At 100 pM factor Xa (Fig. 3, open circles), TFPI (2.5 nM) accounts for 90% inhibition of the rate of
thrombin generation. The inhibition of prothrombinase
activity became negligible when the factor Xa concentration approached
the TFPI concentration (2 nM factor Xa and 2.5 nM TFPI).
Effect of TFPI on Thrombin Generation in the Absence of Factor VIII
The influence of the factor IXa·factor VIIIa pathway on
factor Xa generation in reactions initiated by factor VIIa·TF in the presence of TFPI was studied by omitting factor VIII, thus mimicking the condition of hemophilia A. The experiment performed was otherwise identical to that shown in Fig. 1. Similar results were obtained when
factor IX was omitted from the mixture (results not shown). Thrombin
generation curves in the absence of factor VIII are shown in Fig.
4.
The propagation rate is decreased in the absence of factor VIII;
however, traces of factor VIIa·TF (0.25 pM) ultimately
result in accumulation of significant prothrombinase (Fig.
4A, inverted triangles). Significant TFPI
inhibition of thrombin formation is observed in the absence of factor
VIII (Fig. 4B). In the presence of 2.5 nM TFPI,
concentrations of factor VIIa·TF below 5 pM
(triangles) did not result in significant thrombin
generation. This implies that the explosive thrombin generation that
occurs at 5 pM factor VIIa·TF in the presence of factor
VIII in Fig. 1B is completely dependent upon the factor
VIIIa·IXa pathway. At 20 pM factor VIIa·TF in the
absence of factor VIII (Fig. 4B, squares) the
maximum rate of thrombin generation was inhibited 93% by TFPI. The
virtually linear rate of thrombin generation of 50 nM
min
1 observed at 20 pM factor VIIa·TF
suggests that a limited amount of factor Xa is formed before the factor
VIIa·TF activity is eliminated. This rate of thrombin generation
corresponds to the presence of 10 pM
prothrombinase. From the data shown in Fig. 3 it follows that ~90% of the factor Xa formed is inhibited by TFPI under these conditions, indicating the formation of approximately 100 pM of factor Xa in total. The rate of thrombin generation
by 20 pM factor VIIa·TF in the absence of TFPI indicates
the presence of 150 pM factor Xa after 1 min. Based upon
the rate of 150 pM min
1 of factor Xa
generation in the absence of TFPI, a concentration of 1500 pM factor Xa is expected at 10 min if TFPI had not
inhibited the factor VIIa·TF activity. Instead, only 100 pM factor Xa is produced, consistent with a 93% inhibition
of factor Xa generation at 10 min. The rate of thrombin generation,
however, does not significantly change after the first 2 min of the
reaction, leading to the conclusion that following the initial
formation of 100 pM factor Xa during the first 2 min of the
reaction, TFPI completely inhibited subsequent factor Xa generation.
These data provide evidence that in the reconstituted model TFPI
functions by inhibiting both factor Xa and factor VIIa·TF activity.
Explosive thrombin generation does occur in the presence of TFPI and
the absence of factor VIII at factor VIIa·TF concentrations
100
pM (Fig. 4B, circles).
A comparison of the effect of TFPI on thrombin generation in the
absence and presence of factor VIII reveals interesting observations regarding the influence of TFPI in hemophilia A. A plot of the maximum
effective prothrombinase concentration formed during the reactions versus the initiating factor VIIa·TF
concentration (Fig. 5, data obtained from Figs. 1 and 4)
shows that in the hemophilia A situation (Fig. 5B) TFPI
(open squares) inhibits thrombin generation at low factor
VIIa·TF to less than 1% of the rate observed in its absence
(filled circles). In comparison, when factor VIII is present
(Fig. 5A) the effect of TFPI (open squares) on
the formed prothrombinase activity is limited. These results
emphasize the importance of the factor VIIIa·factor IXa pathway in
overcoming TFPI-mediated inhibition of factor VIIa·TF-initiated
thrombin generation and also demonstrates the potency of TFPI in
inhibiting thrombin generation at low initiator concentrations.
Concentration Dependence of TFPI Inhibition of Thrombin Generation
Thrombin generation initiated by factor VIIa·TF
(1.25 pM) in the presence (Fig.
6A) and absence (Fig. 6B) of
factor VIII was only inhibited at concentrations of TFPI 0.5
nM (filled squares). In the absence of the
factor VIIIa·factor IX pathway (Fig. 6B), the rate of
thrombin generation was decreased 80% by 1 nM TFPI (triangles), while at 2.5 nM the rate of
thrombin generation was only 1% of the control value
(diamonds). TFPI at 5 nM totally abolished
thrombin generation in the absence of the factor VIIIa·factor IXa
pathway (Fig. 6B, filled circles). When factor
VIII was present, the rate of thrombin generation was inhibited 60% by
1 nM TFPI, and a slight increase in the lag time was
observed (Fig. 6A, filled triangles). At 2.5 nM TFPI (diamonds), the initiation phase was doubled, when compared with the reaction in the absence of inhibitor, and the propagation rate was decreased by 70%. At 5 nM
TFPI the lag time was increased from 60 to 240 s, and the rate of
propagation was decreased by 90% (Fig. 6A, filled
circles).
Comparison of the Individual and Combined Effects of TFPI and AT-III on Factor VIIa·TF-triggered Thrombin Formation
A
comparative study was performed analyzing the effects of TFPI (2.5 nM) and AT-III (3.4 µM) at their respective
physiological concentrations on thrombin generation initiated by traces
of factor VIIa·TF. In the reconstituted procoagulant system, thrombin
generation initiated by 1.25 pM factor VIIa·TF in the
absence of inhibitors resulted in explosive thrombin generation after a
30-s lag time (Fig. 7, filled squares).
Immunoblotting showed complete prothrombin consumption at 3 min (Fig.
8A), which was in agreement with generation of maximum thrombin activity (Fig. 7, filled squares).
Immunoblotting for prothrombin fragments after SDS-PAGE under reducing
conditions (Fig. 9A) showed only limited
formation of prethrombin-2 (Mr 37,000) as
compared with generation of the thrombin B-chain
(Mr 30,000) derived from meizothrombin,
meizothrombin-des-F1, and -thrombin. The generation of meizothrombin
and meizothrombin-des-F1 are confirmed by the generation of
immunoreactive bands consistent with fragment 1.2A-chain
(F1.2A) (Mr 47,000) of
meizothrombin and fragment 2A-chain (F2A)
(Mr 18,000), the latter formed by
autoproteolysis of meizothrombin at Arg156 (25), leading to
the release of fragment 1 and the formation of meizothrombin-des-F1.
These data, together with the lack of prethrombin-2, indicate that
thrombin generation proceeded primarily by cleavage of prothrombin at
Arg320, forming meizothrombin, followed by cleavage at
Arg271, resulting in the formation of
-thrombin.
Accordingly, active site blotting using biotinylated-FPR-ck (Fig.
10A) shows formation of the
meizothrombin-derived meizothrombin-des-F1. Films exposed for a longer
interval demonstrate the initial generation of meizothrombin (not
shown). Prothrombin is also subject to cleavage by the various thrombins at Arg156 (25). This results in the
formation of prethrombin-1 that lacks the GLA domain containing
fragment 1. The inability to interact with a membrane via the GLA
domain results in reduced rates of activation of prethrombin-1 and
meizothrombin-des-F1 by prothrombinase.
The addition of 2.5 nM TFPI (Fig. 7, open circles) to the reaction increased the initiation phase from 60 to 150 s and reduced the rate of thrombin generation in the propagation phase by 70%. The point of total prothrombin consumption was delayed to 10 min as shown by immunoblotting (Fig. 8B), which is consistent with the thrombin activity data. TFPI did not seem to change the thrombin generation pathway, since meizothrombin products prevail on the reduced immunoblot and on the nonreduced active site blot in the presence of TFPI (Figs. 9B and 10B). Maximum thrombin activity is reached (Fig. 7) when a considerable amount of the Mr 50,000 component is present in the reaction (Fig. 8B, 10 min). These data suggest that the majority of the Mr 50,000 intermediate is meizothrombin-des-F1, which expresses activity toward the synthetic substrate in the thrombin activity assay.
The addition of 3.4 µM AT-III (Fig. 7, open
squares) to the reaction in the absence of TFPI did not
significantly influence the lag time of thrombin generation. However,
AT-III reduced the rate of appearance of active thrombin by 70%.
Thrombin activity reached a peak of 600 nM after 3 min,
followed by a decline of thrombin activity with an observed
t1/2 of approximately 60 s. At 10 min,
essentially all thrombin activity was inhibited. Immunoblotting for
prothrombin products showed that AT-III reduced the rate of prothrombin
consumption by ~50%, resulting in a slight delay in prothrombin
consumption compared with the control reaction (Fig. 8, compare
A and C). After the appearance of thrombin
activity a Mr 97,000 immunoreactive band
appeared, indicating formation of an -thrombin·AT-III complex.
Upon longer exposure of the immunoblot, a reactive band was also
detected at Mr 120,000, indicating the formation
of a meizothrombin-des-F1·AT-III complex. The increase in intensity
of the
-thrombin·AT-III band parallels the decrease in thrombin
activity. Similarly, the transient incorporation of biotinylated FPR-ck
into the different forms of thrombin (Fig. 10C) also
parallels the peak of thrombin activity (Fig. 7, open squares). In general, the formation of thrombin·AT-III complexes paralleled the decline in thrombin activity; however, a significant amount of noncomplexed, free thrombin B-chain is observed in the immunoblot after 20 min (Fig. 9C). No thrombin activity is
detectable at this stage of the reaction, indicating that this
thrombin, while inhibited by AT-III during the reaction, is present as
an SDS-reversible thrombin·AT-III complex.
Thrombin generation was nearly abolished by the combination of 2.5 nM TFPI and 3.4 µM AT-III when the reaction
was initiated by 1.25 pM factor VIIa·TF (Fig. 7,
open diamonds). However, some active thrombin was still
generated at a rate of 1.4 nM min1. A maximum
of 14 nM thrombin was reached at 10 min, after which a
stable level of thrombin was maintained for up to 15 min. This was
followed by a slow decline in thrombin activity so that after 20 min 11 nM thrombin was still present. Significant prothrombin consumption was not observed, and densitometry of the prothrombin band
showed no significant decrease over the 20-min period evaluated (Fig.
8B). Meizothrombin-des-F1 and prethrombin-1 were formed in
time, and on longer exposures of the immunoblot a
Mr 120,000 reactive band is observed, indicating
the formation of meizothrombin-des-F1·AT-III complexes. Consistent
with this is the presence of prothrombin fragment 2-A observed starting
at 7 min on the reduced immunoblot (Fig. 9D). The relatively
stable activity of the thrombin formed under these conditions seems to
be the result of the activation of prothrombin and the inactivation of
thrombin occurring at similar rates, producing a thrombin steady
state.
TFPI and AT-III when added individually decrease the maximum rate of thrombin generation by 70%. The thrombin generation rate in the presence of both TFPI and AT-III was expected to be 10% of the control, or 100 nM/min, if the effects of TFPI and AT-III were multiplicative. However, the observed thrombin generation rate was 1.4 nM/min in the presence of both TFPI and AT-III. This result indicates that TFPI and AT-III together are ~70-fold more potent at inhibiting the reaction than the inhibitors acting separately. Thus, the two inhibitors, acting in concert, seem to provide a synergistic inhibitory effect.
In the absence of inhibitors, factor V activation is a major event
occurring during the initiation phase of the reaction (17). The effects
of TFPI and AT-III on factor V activation were studied by
immunoblotting with monoclonal HFV-17, which recognizes intact factor V and the factor Va heavy chain (residues 307-506). In the
control experiment and in the presence of AT-III, factor V was
partially activated at 30 s (Fig. 11A
and C). At 60 s, complete cleavage of factor V had
occurred, indicated by the disappearance of single chain factor V
(Mr 330,000) and the appearance of the heavy
chain of factor Va (Mr 105,000) (Fig. 11,
A and C). When TFPI was added, factor V cleavage
was delayed until 60 s but complete at 120 s (Fig.
11B). The activation of factor V in reactions with the
combination of AT-III and TFPI was only slightly slower than that
observed with TFPI alone (Fig. 11D). These data suggest that factor V is a preferred substrate for thrombin in the reaction, since
minimal amounts of thrombin produced during the initiation phase are
still able to quantitatively generate the heavy chain.
Effect of HC-II on Thrombin Generation
The experiment shown
in Fig. 12 compares the effects of HC-II and AT-III, at
their respective physiological concentrations, on thrombin formation.
HC-II (1.38 µM) does not influence thrombin generation
initiated by 1.25 pM factor VIIa·TF (Fig. 12,
filled squares). Subsequently, a slow decline in the
thrombin concentration is observed. AT-III (3.4 µM)
inhibits the rate of thrombin formation, and in contrast to the HC-II
case, the subsequent decrease in thrombin activity is much faster
(open squares). The combination of AT-III and HC-II resulted
in a thrombin generation curve identical to that observed with AT-III
alone (Fig. 12, diamonds), indicating that the contribution
of HC-II to thrombin inhibition is insignificant in the presence of the
faster acting AT-III.
The Dependence of Thrombin Formation on the Factor VIIa·TF Concentration in the Presence of both TFPI and AT-III
The
influence of varying factor VIIa·TF concentrations on thrombin
generation in the presence of both TFPI and AT-III is shown in Fig.
13. Thrombin generation occurred slowly when initiated with 10 pM or lower concentrations of factor VIIa·TF
(Fig. 13, triangles). Explosive thrombin generation was
observed at 25 pM or higher concentrations of factor
VIIa·TF (Fig. 13, squares). A plot of the maximal rate of
thrombin generation as a function of the factor VIIa·TF concentration
(based on the data shown in Fig. 13) is shown in Fig.
14A. The dramatic increase in the maximal rate of thrombin generation following initiation by factor VIIa·TF concentrations >10 pM defines a factor VIIa·TF
concentration threshold for explosive thrombin generation. This
observation is in marked contrast to that observed in the absence of
inhibitors in which only the initial phase of the reaction was
sensitive to factor VIIa·TF. These results clearly demonstrate the
threshold-limited nature of the reconstituted model in the presence of
TFPI and AT-III. This threshold in the factor VIIa·TF concentration
curve defines a "go" or "no go" switch for the blood
coagulation reaction. When the threshold (10-20 pM) is
overcome, the amount of thrombin made available over the first 10 min
is virtually independent of the initiator (factor VIIa·TF)
concentration (Fig. 14B).
From the experiments shown in Figs. 7, 8, 9, 10, 11 and 13 it follows that AT-III
must have an effect on factor Xa generation. An illustration of this is
shown in Fig. 15. The uninhibited factor Xa generation
rate (open circles) is decreased by 50% in the presence of
AT-III (open squares) during the initial 3 min of the
reaction. However, after 3 min the rates of factor Xa generation become equal regardless of the presence of the inhibitor. This result suggests
that the ability of AT-III to inhibit factor Xa is restricted to the
early beginning of the reaction. This observation is consistent with
the conclusion that factor Va protects factor Xa from inactivation by
AT-III (11, 12). Thus, subsequent to its activation, factor Va reduces
the potential for AT-III to inhibit factor Xa. The rate of factor Xa
formation at the later stages of the reaction is probably limited by
the concentration of active factor VIIIa in the reaction. Factor VIIIa
activity is only transiently present because of the dissociation of its
polypeptide chains (26-28) and/or proteolytic inactivation (29-33).
The observation that the ability of AT-III to inhibit factor Xa
generation is limited to the initial stage of the reaction is of major
importance with respect to the proposed synergistic action of AT-III
and TFPI. TFPI significantly delays factor V activation, thereby
increasing the ability of AT-III to inhibit factor Xa.
TFPI is a major inhibitor of thrombin generation, extending the
"lag" time, or initiation phase, of thrombin generation and reducing the rate of thrombin generation during the propagation phase.
The delay in the propagation phase of thrombin generation shows that
TFPI exerts a relatively rapid inhibitory effect on the reaction, which
is associated with delayed factor V activation. In the presence of TFPI
as the only inhibitor, the factor IXa·factor VIIIa complex ultimately
generates sufficient factor Xa, resulting in a bolus of
prothrombinase activity independent of the initiator concentration. In the absence of the factor IXa·factor VIIIa pathway, TFPI reduces the maximally formed prothrombinase activity to
1% of that observed in the absence of the inhibitor at low (5
pM) factor VIIa·TF concentrations. Thus, the propagation
phase of thrombin generation in the presence of TFPI is totally
dependent upon factor IXa·factor VIIIa activity at low concentrations
of factor VIIa·TF. These results provide quantitative support for the
hypothesis that the failure of the hemostatic response upon injury in
patients with hemophilia A or B is, in part, caused by the inactivation
of low concentrations of factor VIIa·TF by TFPI (34). In flow studies
using purified proteins Repke et al. (35) showed that in the
presence of TFPI, ongoing factor Xa generation by factor VIIa·TF was
only obtained in the presence of the factor IXa·factor VIIIa
pathway.
Several groups have reported the rate constants for the inhibition of factor Xa by full-length TFPI (36-38), and a recent study by Jesty et al. (39) reports the rate constants for the inhibition of the factor VIIa·TF complex by the preformed factor Xa·TFPI complex. The complexity of the procoagulant reactions (no steady state conditions established and enzyme occupation by multiple substrates) combined with the complexity of the inhibition reactions by TFPI makes it difficult to determine whether the observed inhibition of the reaction by TFPI can be explained by the reported kinetic constants. Significant (~90%) inhibition of factor VIIa·TF activity by TFPI·factor Xa was observed by Jesty et al. (39) after 60 s. This rapid effect of TFPI on factor VIIa·TF is in agreement with the rapid effect of TFPI on the initiation of the reaction in the reconstituted model.
AT-III inhibits serine proteases by trapping the enzyme at an intermediate stage of proteolysis of the Arg393-Ser394 peptide bond (40-42). Speculation based upon the relatively high (µM) physiological concentrations of AT-III compared with the trace amounts (2.5 nM) of TFPI in the circulation have suggested a potential role for AT-III as an inhibitor of factor VIIa·TF. However, no empirical comparisons on the relative potencies of TFPI and AT-III as inhibitors of factor VIIa·TF-dependent thrombin generation have been reported. The inhibitor AT-III displays a completely different profile of inhibition compared with TFPI. At 1.25 pM factor VIIa·TF, no increase in lag time is observed, while the rate of prothrombin consumption during the propagation phase was reduced by 50% in the presence of physiological concentrations of AT-III. These data demonstrate that full-length TFPI is the major inhibitor of factor VIIa·TF-initiated thrombin formation, while AT-III has no significant influence upon the initiation phase of the reaction, which is almost totally a function of the factor VIIa·TF concentration. The inhibition of factor VIIa bound to TF by AT-III (13) is too slow to significantly inhibit the reaction during its early phase.
In experiments initiated with 5 pM factor VIIa·TF, AT-III inhibited factor Xa generation by 50% during the initiation phase. This is consistent with a slight decrease in prothrombin consumption observed in the presence of AT-III. However, the reduced amounts of factor Xa and factor IXa generated by factor VIIa·TF when TFPI is present seem to become efficiently scavenged by AT-III, thus preventing explosive thrombin generation (Fig. 7, open diamonds).
Factor Va has been reported to protect factor Xa from inactivation by AT-III (11, 12). Factor V is cleaved by 2 min in the reaction and the picomolar amounts of factor Xa generated contemporaneously are saturated by this excess of factor Va and thus relatively protected against inactivation by AT-III. However, factor Xa inactivation can occur prior to factor V activation and/or prothrombinase complex formation. In this respect, the observed delay of factor V activation in the presence of TFPI may play an important role in the potentiation of the action of AT-III by TFPI (Fig. 7).
In the absence of TFPI, protection of factor IXa by factor VIIIa against inactivation by AT-III is probably of less importance because of the relatively low concentration and the transient presence of active factor VIIIa due to its dissociation (26-28) and/or proteolytic inactivation (29-33). In contrast to prothrombinase, the amount of factor VIIIa cofactor is probably the limiting factor for the formation of the factor IXa·factor VIIIa complex. In the presence of TFPI, however, the observed inhibitory effect of AT-III is probably also due to scavenging of the trace amounts of factor IXa generated. In this model the threshold for explosive thrombin generation in the presence of 2.5 nM TFPI and 3.4 µM AT-III is ~20 pM factor VIIa·TF. The threshold level coincides with the minimal factor VIIa·TF concentration (20 pM) needed to generate considerable thrombin in the presence of TFPI and the absence of the factor IXa·VIIIa pathway (Fig. 4). This is an indication that AT-III prevents explosive thrombin generation by inhibiting the factor IXa·VIIIa pathway. This coincidence may, however, also be the result of a change in the reaction in the range of 10-20 pM from a reaction with no lag time to a reaction with a considerable lag time, which allows the effects of the additional factor Xa generation by factor IXa·factor VIIIa and the effects of the inhibitors to become more prominent. Overall, the effects of TFPI and AT-III acting in concert seem to result in a synergistic inhibition of thrombin generation at low concentrations of initiator, leading to a 70-fold higher inhibition than expected if their combined action would have been multiplicative. Although it seems that TFPI and AT-III act in synergy under these conditions, such claims of synergism must be made with caution without full knowledge of the mechanisms involved (43). At present the complexity of the reaction does not allow a proof of true synergism between TFPI and AT-III.
The major inhibitory effect of TFPI on factor VIIa·TF-initiated thrombin generation in the reconstituted model predicts that a TFPI deficiency would be a major risk factor for thrombosis. Titration of the effect of TFPI on thrombin generation revealed that TFPI exerts a significant inhibitory effect at 1 nM (Fig. 6). This TFPI concentration is approximately 50% of the normal plasma concentration, suggesting that an individual with a 50% TFPI level would derive a significant inhibitory benefit. Our data suggest, however, that a homozygous TFPI deficiency would result in massive thrombin formation, which may not be compatible with life. However this hypothesis does not take into account that the dependence on TFPI of inhibition of factor VIIa·TF-initiated thrombin generation might be less in the presence of the dynamic protein C pathway.
Experiments with physiological concentrations of HC-II (1.38 µM) predict an insignificant role for this inhibitor as compared with AT-III. Thrombin generation proceeds in a similar fashion whether or not HC-II is present. Although a marginal effect of HC-II was observed on the activity of thrombin after the quantitative activation of prothrombin, no additional thrombin-inhibitory potential was observed when HC-II was combined with physiological concentrations of AT-III. This result supports the hypothesis that HC-II is not important as a coagulation inhibitor and is in agreement with the lack of a thrombotic tendency in individuals with reduced HC-II levels (16).
The combined effects of TFPI and AT-III prevent explosive thrombin generation by traces of factor VIIa·TF in the fully reconstituted system. The dramatic change in the rate of thrombin generation over a small change in the initiating factor VIIa·TF concentration (Figs. 13 and 14) clearly demonstrates that significant thrombin generation becomes a threshold-limited event with regard to the initiating factor VIIa·TF concentration in the presence of TFPI and AT-III. The presence of traces of fibrinopeptide A, prothrombin F1·2, and thrombin·AT-III complexes in the plasma of normal individuals indicates that very low, but constant triggering of the coagulation cascade occurs in the unperturbed circulation. The present data suggest that the low level of basal activation of the coagulation system is largely controlled by the combined action of inhibitors like TFPI and AT-III, which prevent this apparent basal activity from turning into massive thrombin formation.
Portions of this work were presented at the 37th annual meeting of the American Society of Hematology, December 1-5, 1995, Seattle, WA (44).
We thank Dr. Kirk Johnson (Chiron Corp.) for the generous gift of recombinant TFPI; Dr. Shu Len Liu and Dr. Roger Lundblad (Hyland Division, Baxter Healthcare Corp.) for providing us with recombinant factor VIII and recombinant tissue factor; Dr. William Church (Antibody Core, Department of Biochemistry, University of Vermont) for providing the monoclonal antibodies; Dr. Richard Jenny (Haematologic Technologies) for providing FPR-ck; and Dr. Michael Kalafatis for critical reading of the manuscript. We thank Kelly Begin and Neal Golden for technical assistance.