(Received for publication, October 10, 1996, and in revised form, January 2, 1997)
From the Division of Molecular Transport, Department
of Internal Medicine, University of Texas Southwestern Medical Center,
Dallas, Texas 75235-9121, § Preclinical Research and
Development, Astra Hässle AB, S-431 83, Mölndal, Sweden,
and the ¶ Department of Biochemistry and Biophysics,
Göteborg University, Medicinaregatan, 9C, S-41390,
Göteborg, Sweden
The vacuolar type proton pump of clathrin-coated vesicles has a multisubunit ATP hydrolytic center that is peripheral to the membrane. Polypeptides present in this domain include the well characterized subunits A, B, C, D, E, and F; SFD, a dimer composed of 50- and 57-kDa polypeptides; and polypeptides termed G and H. Of these, subunits A, B, C, and E have been shown to be necessary but not sufficient for significant ATPase activity; in addition, either polypeptide G or H is also required for ATP hydrolysis (Xie, X.-S. (1996) J. Biol. Chem. 271, 30980-30985).
In this study, the polypeptides G and H were purified and directly sequenced. Subsequent molecular analysis has revealed that these proteins are isoforms, which we designate G1 and G2. The cDNAs encoding the rat and bovine brain and chicken osteoclast forms of G1 have been cloned. The open reading frames of the rat and bovine clones encode hydrophilic proteins of 118 amino acids that differ at only five residues; bovine G1 has 36% identity with VMA10, a component of the proton channel of yeast. Northern blot analysis revealed a 1.0-kilobase pair transcript encoding G1 in bovine brain, kidney, heart, and spleen. The cDNA encoding bovine polypeptide H was cloned and sequenced, revealing this protein to be 64% identical to G1, constituting isoform G2. In Northern blot analysis, a single 1.7-kilobase pair transcript hybridized with a probe to G2 in brain, but not in heart, kidney, or spleen. An antibody against a bovine G1-specific domain reacts with V pump from bovine brain, kidney, and chromaffin granule, whereas an anti-G2 antibody reacts only with proton pump from brain. The bovine forms of G1 and G2 were subsequently expressed in Escherichia coli and Sf9 cells, respectively, and purified to homogeneity. Reconstitution of ATP hydrolysis was achieved by combination of recombinant subunits A, B, C, and E with either recombinant G1 or G2, demonstrating the role of these isoforms in pump function.
Vacuolar, or V-type, proton pumps are remarkable for their wide distribution within eukaryotic cells, where they are found in most organelles, as well as for their intense phylogenetic conservation. Physiologically, these pumps are responsible for urinary acidification and bone resorption and participate in the respiratory burst phenomenon of macrophages. At a constitutive level, V-type proton pumps are essential for glycosylation in Golgi membranes, degradation of cellular debris in lysosomes, and processing of endocytosed receptor-ligand complexes in endosomes. In regulated secretory pathways, these proton-translocating ATPases energize neurotransmitter and catecholamine storage and facilitate processing of insulin as well as packaging of histamine and heparin (1-6).
Much is now known about the general structure of V-pumps. In all instances, they are multisubunit hetero-oligomers with a large peripheral sector, which catalyzes ATP hydrolysis, and a transmembranous proton channel (1-6). Investigation of the structure and function of the V-pump of clathrin-coated vesicles has revealed that the peripheral sector is composed of polypeptides of 70, 58, 40, 33, and 14 kDa, termed subunits A, B, C, E, and F, respectively (7-11). Recent work has also identified other components of this sector. These include a dimer composed of 57- and 50-kDa polypeptides termed SFD (ub-ifty-eight kDa imer) (12), subunit D (13), and two polypeptides that we have designated G and H (14).
Definition of these components of the catalytic sector has been approached in several ways. It has been shown that the peripheral components of V-type pumps can be released from the proton channel by incubation with chaotropic agents, and/or nucleotides at 4 °C (15-16). These released polypeptides have been termed V1, in analogy to the peripheral sector of the mitochondrial ATPase, which was termed F1, (for actor 1) (1). There has been no report of ATPase activity associated with these released subunits, and thus V1 constitutes a group of peripheral subunits that are hypothesized to catalyze ATP hydrolysis. This supposition is well supported by nucleotide labeling experiments and identification of signature sites for ATP binding/hydrolysis in two of these peripheral components (subunits A and B) (17-21).
By another approach, we have sought to define by a functional analysis the elements responsible for ATP hydrolysis in V-type pumps. This has required dissociating the enzyme, isolating the separated subunits and/or subcomplexes, and determining by add-back experiments the requirement for ATP hydrolysis. Dissociation of the enzyme by several biochemical procedures (e.g. urea treatment) results in a change in the characteristics of the ATPase activity of the pump; namely, the enzyme loses its capability of hydrolyzing MgATP and switches to a form capable of only CaATP hydrolysis (22). We have termed this peripheral catalytic sector VC, to distinguish these functional (and possibly structural) features from those of V1. Recently, evidence has emerged that the catalytic sector of V pumps exists as a multisubunit subcomplex, has Ca2+-activated ATPase activity, and reversibly associates with the transmembranous sector to increase pump number under physiological stimuli (23).
Our goal of defining the components of the pump responsible for ATP hydrolysis has centered upon identification of the minimal polypeptide requirements for CaATPase activity. This has resulted in the findings that four subunits (A, B, C, and E) are necessary, but not sufficient, for significant ATPase activity (7-10). Recently, it was found that an additional subunit was also necessary for ATPase activity and that either polypeptide G or H could fulfill this need. These studies were performed with recombinant subunits A, B, C, and E and biochemically prepared G and H polypeptides, and they were further remarkable for the fact that both CaATPase and MgATPase activity were reconstituted by these components (14).
To further explore the roles of these polypeptides in proton pump function, the molecular identities of polypeptides G and H have been investigated. Direct peptide sequencing, and cloning of their cDNAs reveal these components be highly homologous to each other and also to subunit G of the V pumps of yeast (24), Manduca sexta (23), and bovine chromaffin granule (25). These isoforms, which we term G1 and G2, differ in their tissue distributions. Northern blot analysis reveals that G2 is present in brain but not in kidney, liver, or spleen, whereas G1 is present in all tissues examined. G1 and G2 have been expressed in Escherichia coli and Sf9 cells, respectively, and purified to homogeneity. Reconstitution of MgATPase and CaATPase activity was achieved with recombinant subunits G1, G2, A, B, C, and E. In these experiments, it was determined that either G1 or G2, but not both, are required for reconstitution of ATP hydrolysis.
Restriction enzymes, T4 DNA ligase,
and a nick translation kit for DNA probe labeling were purchased from
Boehringer Mannheim; the GeneAmp PCR reagent kit with Thermus
aquaticus Taq DNA polymerase and DNA sequencing materials and
reagents were from Perkin-Elmer; a bovine brain cDNA library and a
rat brain cDNA library in ZAPII, their host E. coli
cells BB4 and XL1-Blue, and helper phage R408 were from Stratagene;
E. coli expression vector pET16b and its host cell BL21(DE3)
were from Novagen; baculovirus expression vector pVL1392 and
baculovirus (AcNPA) DNA were from PharMingen; [
-32P]dCTP, [
-32P]ATP, and an ECL kit
for Western blot analysis were from Amersham Corp.; nitrocellulose
membranes for plaque lift were from Millipore Corp.; and chemicals for
SDS-PAGE were from Bio-Rad. Bovine adrenal chromaffin granule membranes
were the generous gift of Dr. Joseph Albanesi (University of Texas
Southwestern Medical Center at Dallas). All other reagents were
obtained from Sigma.
Polypeptides G and H were separated by SDS-PAGE (15% acrylamide), and proteins were electrophoretically transferred to Immobilon Psc filters, from which both polypeptide bands were excised and digested with trypsin (26). Released peptides were separated by reverse-phase high pressure liquid chromatography using a 2.1 × 150-mm RP300 column (Perkin-Elmer) and were subjected to automated Edman degradation with a model 477A amino acid sequencer (Applied Biosystems, Inc.) using the manufacturer's standard program and chemicals.
Cloning of cDNAs Encoding Subunit G1 from Rat, Bovine, and Chicken SourcesZAPII phage DNA from an amplified rat brain
cDNA library was purified by standard methods (26) and used as a
template for PCR, which was performed with 1 µg of purified
ZAPII
DNA and 20 pmol of two synthetic oligonucleotide primers:
5
-GAAAGAACCGGAGGCTGAAGC-3
and 5
-CGGAAGTATGTCTGGAGGATGG-3
. The
sequences of these primers were based on direct peptide sequences and a
homologous human EST sequence C-IHF03 (EMBL accession number Z43645[GenBank]). A
183-bp1 PCR product was cloned into
pCRTMII vector using a commercial TA cloning kit
(Invitrogen). After positive selection by restriction enzyme digestion,
the insert was excised with EcoRI and purified by
preparative agarose gel electrophoresis.
The 183-bp PCR fragment was labeled with [-32P]dCTP by
nick translation and used to screen a rat brain cDNA library in
ZAPII that had been transfected into E. coli strain BB4.
Plaques were transferred to nitrocellulose membranes by a double-lift
procedure (27). The membranes were then prehybridized for at least
4 h at 60 °C in a solution containing 5 × SSC, 5 × Denhardt's solution, 0.1 mg/ml sheared salmon sperm DNA, and 0.1%
SDS. Hybridization was performed at 42 °C overnight with the same
solution plus labeled probe, which was added at a concentration of
5-10 × 105 cpm/ml of hybridization solution.
Duplicate positive clones were rescreened through one or more cycles
until purified plaques were obtained.
Subunit G1 cDNA was cloned from a bovine brain cDNA library in
ZAPII, using the same conditions for screening the rat brain library, except for a different probe, which was a 685-bp insert of a
clone (R3-1) encoding rat brain subunit G1.
cDNA encoding chicken subunit G1 was cloned from a ZAP Express
cDNA library prepared from chicken
osteoclasts2 using procedures similar to
those described for cloning the cDNAs encoding the rat and bovine
forms of subunit G1. Specifically, 1 µg of
ZAP Express phage DNA
was used as template, and two synthetic oligonucleotides
(5
-ATGGCIAG(T/C)CA(A/G)TCIGAGGGIAT-3
and
5
-CGGTAITT(T/C)TC(G/A)TGGATTTCIGG-3
) were used as primers
for PCR amplification. The resulting 342-bp PCR product was sequenced
and found to be homologous to the cDNA encoding the rat and bovine
forms of subunit G1. Subsequently, probe labeling and screening were
carried out as described for cloning of the rat brain cDNA, except
E. coli strain XL1 Blue MRF
was infected with the
ZAP
Express lysate. Purified clones were in vivo excised into
pBK-CMV using helper phage ExAssist and E. coli strain
XLOLR. Four positive cDNA clones were identified as chicken subunit
G1. Only one clone (COG03) contained the putative start codon and a
28-bp upstream sequence.
Two oligonucleotide primers,
5-AAGGAGGAGGCICA(A/G)ATGGA(A/G)GT-3
and
5
-CTGCATICC(C/T)TGIAC(C/T)TGIC-3
, were synthesized in accord
with two of the peptide sequences. Deoxyinosine (I) was used in the
third position of some codons with a degeneracy of two or more.
ZAP
phage DNA from amplified bovine brain cDNA library was purified by
standard methods (27) and used as a template for polymerase chain
reaction. A 283-bp PCR product was cloned into pCRTMII
vector. The insert was excised with BamHI and
EcoRV, purified by preparative agarose gel electrophoresis,
and used to screen a bovine brain cDNA library by a double-lift
procedure, as described above.
Inserts from all positive clones were excised and cloned into pBluescript or pBK-CMV, with helper phage R408. Plasmid DNA was prepared by alkaline lysis, and DNA sequencing reactions were carried out with the ABI PRISMTM dye terminator cycle sequencing ready reaction kit with Ampli Taq DNA polymerase, and subjected to sequencing analysis using a model 377 ABI PRISM DNA sequencer. The positive clones were fully sequenced in both orientations using T7, T3 promotor sequences, and sequence-specific oligonucleotides as primers. DNA and protein data base searches were performed using a PC/GENE-based program.
Northern Blot AnalysisPoly(A)+ RNA (2 µg)
from designated bovine tissues was denatured and fractionated by 1%
formaldehyde-agarose gel electrophoresis and transferred to a
Zeta-Probe blotting membrane (Bio-Rad). After baking at 80 °C in a
vacuum oven for 1 h, the membrane was prehybridized for 4 h
at 50 °C in a solution consisting of 50% formaldehyde, 1.5 × saline/sodium phosphate/EDTA, 1% SDS, 0.5% nonfat dry milk, 0.5 mg/ml
denatured salmon sperm DNA. The probes for subunits G1 and G2, and
-actin were labeled with [
32P]dCTP by nick
translation and added to the hybridization solution at a concentration
of 1 × 106 cpm/ml of solution. Hybridization was then
carried out at 50 °C overnight. The membrane was sequentially washed
for 15 min at room temperature with 2 × SSC and 0.1% SDS,
0.5 × SSC and 0.1% SDS, and subsequently 0.1 × SSC and
0.1% SDS. A final wash was carried out at 60 °C with 0.1 × SSC and 0.1% SDS for 30 min, and autoradiography was performed with an
intensifier screen at
80 °C for 5 days.
The
coding region for bovine subunit G1 was amplified by PCR using cloned
cDNA B33-1 as template and two synthetic oligonucleotides (5-AGCCATACATATGGCCAGTCAGTCGCAGGG-3
and
5
-GCAATAGGAGATCTAGAGCTGCCTCTATCCATTTATGCGG-3
) as primers, which were
designed to contain NdeI and BglII restriction sites and initiator and stop codons at the 5
-ends, respectively, to
enable cloning into the bacterial expression vector pET16b. The
amplification reaction was performed in a Gene Machine thermal cycler
using the following conditions: 1 min at 94 °C and 5 min at
68 °C, for a total of 30 cycles. The PCR product was
size-fractionated by agarose gel electrophoresis, from which a single
380-bp fragment was purified; the fragment was identical to the
cDNA sequence as determined by direct DNA sequencing. Expression
vector pET16bG1 was constructed by replacing the NdeI and
BamHI fragment of pET16b with the PCR-amplified fragment,
which had been digested with NdeI and BglII. For
expression of subunit G, E. coli strain BL21 (DE3) was
transformed with expression vector pET16bG1 and grown and induced with
IPTG at 37 °C as described (28).
pET16bG1 encodes a fusion protein containing a histidine tag at the amino terminus of the subunit G1, potentially allowing the protein to be readily purified with Ni-NTA resin. However, the expressed subunit G1 was largely aggregated into insoluble form (inclusion bodies) in E. coli, and thus the following purification protocol was developed.
Step 1: Cell Lysis and Isolation of Inclusion Bodies500 ml of IPTG-induced BL21 (DE3) cells harboring pET16bG1 were harvested by centrifugation at 4000 × g for 15 min at 4 °C and resuspended in 6 ml of lysis buffer (50 mM Tris HCl, pH 8.0, 1 mM EDTA, 100 mM NaC1) containing 0.2 mM phenylmethylsulfonyl fluoride and 0.2 mg/ml lysozyme. The mixture was kept on ice and stirred occasionally for 20 min. Subsequently, 300 µl of 20% sodium cholate was added, and the mixture was incubated at 37 °C and continuously stirred with a glass rod for 5-10 min. When the lysate became viscous, 40 µl of DNase I (1 mg/ml) was added. The lysate was kept at room temperature until it was no longer viscous (30-40 min), and then it was centrifuged at 12,000 × g for 15 min at 4 °C. The supernatant was discarded, and the pellet was suspended in 10 ml of denaturing buffer consisting of 6 M guanidium HCl, 0.1 M NaH2PO4, and 10 mM Tris-HCl, pH 8.0. After centrifugation at 12,000 × g for 15 min, the supernatant was used for Ni-NTA resin chromatography.
Step 2: Ni-NTA Resin Chromatography and Renaturation of Recombinant Subunit G1The supernatant from step 1 was mixed with 1 ml of equilibrated Ni-NTA resin (Qiagen), and the mixture was shaken for 1 h at room temperature. After centrifugation, the Ni-NTA resin was loaded into a small column, which was then washed with 120 ml of wash buffer (50 mM NaH2PO4, 300 mM NaC1, 15 mM imidazole, 10 mM Tris-HCl, pH 8.0) containing a continuous urea gradient from 6 to 3 M with a flow rate of 1 ml/min. The protein was eluted with 10 ml of wash buffer containing 3 M urea and 200 mM imidazole, and 1-ml fractions were collected and analyzed by SDS-PAGE.
Step 3: Glycerol Gradient CentrifugationFor further purification and refolding of recombinant subunit G1, selected fractions from Ni-NTA chromatography were loaded onto a 10-20% glycerol gradient, which was prepared in 10 mM Tris-HCl (pH 7.5), 0.5 mM EDTA, 3 mM dithiothreitol, and 1 M urea. After centrifugation at 180,000 × g for 22 h at 4 °C, fractions were harvested from the upper fractions of the gradient, subjected to SDS-PAGE analysis, and used for reconstitution experiments.
Expression and Purification of Subunit G2 in Insect Sf9 CellsThe coding region for subunit G2 of H+-ATPase
was amplified by PCR using cloned cDNA as template and two
oligonucleotides (5-AACAACACATATGGCCAGTCAATCCCAGGGT-3
and
5
-GGAGTCGGATCCTAGGTTAGGTCCTAGGCAG-3
) and cloned into the vector
pET16b. Bacterial expression vector pET16bG2 was constructed by
replacing the NdeI-BamHI fragment of plasmid
pET16b with the PCR product after digestion with the same restriction
enzymes. pET16bG2 was digested with XbaI and
BamHI. The resultant 0.5-kilobase pair fragment, which
contained the entire coding region for subunit G2, was cloned into the
XbaI and BamHI sites of vector pVLL1392 to
generate the baculovirus expression vector pVLL1392-G2.
Spodoptera frugiperda (Sf9) cells were grown in monolayer or in suspension culture at 27 °C in either Grace's or IPL-41 medium with 10% heat-inactivated fetal bovine serum plus 0.1% pluronic polyol F68. Cells were split 1:5 for propagation every 3-4 days. Recombinant baculovirus was generated by co-transfection of Sf9 cells by the Lipofectin method with purified vector pVL1392-G2 DNA and linearized AcNPA viral DNA (9). Positive viral clones were isolated by plaque assay and identified by their ability to direct the expression of subunit G2 protein, as determined by SDS-PAGE and Western blot analysis. For expression and production of recombinant protein, Sf9 cells (2 × 106 cells/ml medium) were infected with pure recombinant baculovirus and grown in suspension at 27 °C for 60-72 h. 500 ml of infected Sf9 cells were harvested by centrifugation at 4,000 × g for 15 min. The cells were then resuspended in 50 ml of lysis buffer consisting of 50 mM Na2HPO4, 300 mM NaCl, 0.25% Tween 20, 0.2 mM phenylmethylsulfonyl fluoride, and protease inhibitor mixture (CompleteTM, Boehringer Mannheim), and sonicated three times for 1 min on ice. The lysate was then centrifuged at 40,000 rpm for 1 h at 4 °C with a Beckman Ti 70 rotor. The supernatant was discarded, and the pellet was suspended in 10 ml of denaturing buffer, consisting of 6 M guanidium HCl, 0.1 M NaH2PO4, and 10 mM Tris-HCl, pH 8.0. After centrifugation at 15,000 × g for 15 min, recombinant subunit G2 was purified by Ni-NTA resin chromatography and refolded, using procedures similar to those described for recombinant subunit G.
Reconstitution and Measurement of ATPase ActivityAll of
these components used for ATPase reconstitution are described herein
and elsewhere (8-10). ATPase activities were measured by the
liberation of 32Pi from
[32P]ATP. The assay solution consisted of 30 mM KCl, 50 mM Tris/MES (pH 7.0), 2.5 mM CaCl2 or MgCl2, and 2 mM [
32P]ATP (200-400 cpm/nmol). ATPase
samples were preincubated with 2.5 µg of phosphatidylserine for 2-5
min at room temperature, and reactions were initiated by the addition
of 100 µl of assay solution. After incubation for 30 min at 37 °C,
the reactions were terminated by the addition of 1 ml of 1.25 N perchloric acid, and the liberated
32Pi was extracted and counted as described
(29).
Two peptides, CEKETQEKMTILQTYFRQNRDE (specific to subunit G1) and CEQATRRQVQGMQSSQQRNRER (specific to subunit G2), were synthesized based on deduced protein sequence, coupled to keyhole limpet hemocyanin, and used for immunization of New Zealand White rabbits as described previously (30). For Western blot analysis, protein samples were separated by 15% SDS-PAGE and transferred electrophoretically to nitrocellulose paper. Immunodetection was performed using immune serum at a 1:5,000 dilution and an Amersham ECL Western blotting system.
Miscellaneous ProceduresBovine brain clathrin-coated vesicle H+-ATPase, its peripheral catalytic sector (VC), and integral membrane proton channel (VB) were purified as described (22, 31, 32). Briefly, VC was prepared by treating purified pump with urea, followed by glycerol gradient centrifugation, and VB was harvested from upper fractions of glycerol gradients used for purification of the intact proton pump. H+-ATPases from bovine kidney and chromaffin granule membranes were isolated as described for the pump of bovine brain clathrin-coated vesicles (22) except for the omission of hydroxyapatite chromatography. Protein determination (33) and SDS-PAGE (34) were performed as reported.
Direct protein sequencing of polypeptides G and H was performed as described under "Experimental Procedures." Shown in Table I are the determined amino acid sequences of tryptic peptides derived from G and H.
|
Data base analysis
using the amino acid sequences of polypeptide G (Table I) identified
several homologous human EST sequences. As the sequences directly
obtained from peptide sequencing were not ideal for generation of
oligonucleotide primers, we used flanking sequences within the EST
clones to guide primer synthesis. As described under "Experimental
Procedures," this led to the isolation of the 183-bp PCR product that
was used to screen a rat brain cDNA library (5 × 105 individual bacteriophage) in ZAPII. Three positive
clones, designated R3-1, R7-1, and R7-2, were isolated; all three
clones had identical sequences at overlapping regions (data not shown).
Among them, clone R3-1 was found to contain the full coding region.
The complete sequence contains 685 base pairs consisting of a 63-bp
untranslated region at the 5
-end, a 265-bp noncoding region at the
3
-end, and a 357-bp open reading frame (Fig.
1A).
To clone the cDNA encoding bovine subunit G1, the entire rat
cDNA fragment (R3-1) was used to screen 5 × 105
phages of a bovine brain cDNA library. Three positive clones (B33-1, B42-1, and B43-1) were identified. Clones B42-1 and B43-1 contained only partial coding regions, and clone B33-1 contained the
entire open reading frame. All positive clones had identical sequences
at the overlapping region of both coding and noncoding regions. The
sequence of clone B33-1 includes a 357-bp open reading frame and
untranslated regions of 76 bp at the 5-end and 542-bp at the 3
-end.
While these studies were being performed, the cDNA sequence of
bovine subunit G1 was published elsewhere (25), and the sequences of
our clones were identical to that published.
To clone the chicken G1, a 342-bp cDNA fragment was used to screen
6 × 105 phages of a chicken osteoclast cDNA
library in ZAP Express. Four positive clones (COG01-04) were
isolated. All clones had identical sequences differing only in the
length of their 5
end (data not shown). Clone COG03 was found to
contain the full coding region. The sequence of clone COG03 from the
chicken osteoclast cDNA library is shown in Fig. 1C. The
sequence of clone COG03 included a 357-bp open reading frame and
untranslated regions of 28 bp at the 5
end and 563 bp at the 3
end.
The primary amino
acid sequences of polypeptide H (Table I) were used to design primers
instrumental in developing a probe, as described under "Experimental
Procedures." After screening 5 × 105 individual
bacteriophages, nine positive clones, designated B3-1, B9-1, B11-1,
B13-1, B15-1, B16-2, B17-1, and B17-2 were isolated. DNA
sequencing demonstrated that clones B3-1, B9-1, B13-1, B14-1, B15-1, and B17-2 contained full coding regions, and all of the positives had identical sequences at the overlapping region of both
coding and noncoding regions, with the exception of clone B3-1, which
had a 19-bp insertion within the coding region. (Since this would
induce a frame shift, this was probably due to a library artifact.).
The full sequence of bovine clone B17-2, which includes a 357-bp open
reading frame and an untranslated region of 51 bp at the 5-end and 955 bp at the 3
-end, is shown in Fig. 1B.
The translations
of open reading frames of the bovine, rat, and chicken subunit G1
clones predict 118-amino acid proteins with calculated molecular masses
of 13,682, 13,710, and 13,552 Da, respectively, which is in close
agreement with the apparent molecular mass of the bovine G1 subunit as
assessed by SDS-PAGE. Such is also the case with bovine G2, where
translation of the open reading frame of its clone predicts a 118-amino
acid protein with a calculated molecular mass of 13,565 Da. Although G1
and G2 are nearly identical in mass, they have isoelectric points of
8.22 and 10.89, respectively, which likely accounts for observed differences in their mobilities during SDS-PAGE (14). Kyte-Doolittle analysis (35) predicted no membrane-spanning domains for G1 or G2. This
characteristic is consistent with the experimental observation in our
laboratory that subunits G1 and G2 copurify with the peripheral
catalytic sector (VC) of the V-type ATPase of brain. The
primary structures of the bovine, rat, and chicken subunits G1 are
highly conserved. Only five amino acid residues were found to be
different among the 118 residues of the rat and bovine forms, and only
18 amino acid residues were different in the bovine and chicken forms.
Data base searches revealed that the predicted sequence of bovine
subunit G1 shares 36, 53, and 100% identity to VMA10 (24), to the G
subunits of the V pump of tobacco hornworm (23), and to the bovine
chromaffin granule (25), respectively. Bovine subunits G1 and G2 share
64% identity. Bovine G2 has 34 and 51% homology with VMA10 and the G
subunit of tobacco hornworm. In general, residues of the amino-terminal half of the protein are more highly conserved than those of the carboxyl-terminal region (Fig. 2).
Expression of Subunit G1 in E. coli and Purification of Recombinant Protein
The open reading frame encoding bovine G1 subunit was
amplified by PCR and cloned into the bacterial expression vector
pET16b, resulting in vector pET16bG1, as described under
"Experimental Procedures." E. coli BL21(DE3) cells
harboring the vector were grown to A600 = 0.7-0.9 at 37 °C. After IPTG induction and lysis, a dominant
protein band was visualized by SDS-PAGE and Western blot (Fig.
3). About 10% of the total protein in induced BL21(DE3) cells was recombinant protein (Fig. 3, lane 3), which
strongly cross-reacted with an anti-G1 antibody (Fig. 3B).
Recombinant subunit G1 was solubilized and purified as described under
"Experimental Procedures" and is shown in Fig. 3, lane
4. It appears to have a higher molecular mass than the native
subunit G1 because of additional factor Xa and histidine residues.
Expression and Purification of Recombinant Subunit G2
Bacterial expression vector pET16bG2, containing the entire
coding region for the subunit G2, was used to express subunit G2 in
E. coli. However, the resultant recombinant protein in
E. coli was subject to severe proteolysis during the
expression. Repeated attempts to control the proteolysis were
unsuccessful. As an alternative, we constructed and purified a
recombinant baculovirus containing the cDNA sequence encoding
subunit G2 that was utilized to transfect insect Sf9 cells. The
recombinant baculovirus directed the expression of a fusion protein
containing a histidine tag at the amino terminus. As shown in Fig.
4, subunit G2 was expressed in Sf9 cells. Production of
recombinant protein reached a maximal level in Sf9 cells after
infection for 72 h, constituting approximately 3-5% of the total
cellular proteins.
Although significant expression of the subunit G2 was obtained in Sf9 cells, the recombinant protein was largely aggregated, requiring the use of a guanidium-denaturing procedure as described under "Experimental Procedures." The purified protein is shown in Fig. 4, lane 4.
Expression of Subunits G1 and G2 in Different TissuesThe
tissue distributions of mRNAs encoding bovine subunits G1 and G2
were investigated by Northern blot analysis, as shown in Fig.
5. A single transcript of 1.0 kilobase pair in bovine brain, heart, kidney, and lung hybridized with the subunit G1 probe
(Fig. 5B), whereas the G2 probe hybridized with a single mRNA band of 1.7 kilobase pairs in bovine brain, but not in heart, kidney, or lung (Fig. 5A). Analysis with -actin probe
revealed that the amount of mRNA used for Northern blot analysis
was roughly equal for all tissues (Fig. 5C).
To further demonstrate the differential expression of subunits G1 and
G2, Western blot analysis was performed as demonstrated in Fig.
6. Subunit G2 was detectable only in the V-ATPase
isolated from bovine brain, and not in the ATPases from the kidney and chromaffin granule (Fig. 6A). Subunit G1 was present in all
pump preparations (Fig. 6B).
A Subunit G Isoform Is Required for Significant ATPase Activities
We have demonstrated that four subunits, A, B, C, and
E, are necessary, but not sufficient, for V-ATPase activity (7-10). To
investigate the role of subunits G1 and G2 in ATP hydrolysis, recombinant components (shown in Fig. 7) were tested for
the ability to support ATP hydrolysis. As demonstrated in Table
II, recombinant A and B subunits supported minor ATP
hydrolytic activity (10) that was not stimulated by the addition of the
recombinant C and E subunits. However, the addition of subunit G1 to
these four recombinant subunits resulted in significant
Ca2+- and Mg2-activated ATPase specific
activities of 0.43 and 1.1 µmol of Pi·mg of
protein1·min
1, respectively. Subunit G2
can replace subunit G1 to support significant Ca2+- and
Mg2+-activated ATPase activities of 0.8 and 2.0 µmol of
Pi·mg of protein
1·min
1;
these specific activities are about two times higher than those achieved by the reconstitution with subunit G1. The differences in
specific activities reconstituted with subunit G1 or G2 are further
illustrated by the titration experiments of Fig. 8.
|
Subunit G Is a Component of the Peripheral Catalytic Sector
Subunits G1 and G2 lack membrane-spanning domains, as
determined by Kyte-Doolittle analysis (35) of the deduced amino acid sequence, supporting the notion that they belong to the peripheral catalytic sector (VC) of the H+-ATPase. In
contrast, subunit G (VMA10) of the yeast and bovine V-ATPases have been
proposed to be membrane-associated (24, 25). To address this issue, we
generated a purified transmembrane sector (Fig. 9,
lane 2). SDS-PAGE and Western blot analysis demonstrated that subunits G1 and G2 copurify with the peripheral sector, and not
with the transmembrane sector (Fig. 9). More importantly, the
reconstitution of ATPase activities in the current studies demonstrates
that subunits G1 and G2 are required for significant ATP hydrolysis,
indicating that the components are part of the catalytic sector.
A definition of the components of V pumps has been approached by several strategies. The finding that subunit A could be labeled with nucleotides led to the view that this component was the site of ATP hydrolysis. Comparisons of V pump architecture with that of the mitochondrial ATP synthetase led to the notion that a prominent peripheral structure of V pumps (V1), was similar to F1 of the mitochondrial ATPase and thus was the catalytic domain of the enzyme. Release of components of V1 from the membrane led to the identification of a collection of polypeptides that lacked ATPase activities but were considered to constitute the catalytic core of the enzyme.
In a different approach, we have sought to define the components of the catalytic sector by reconstituting ATPase activities from isolated subunits. Initially, this entailed attempts at purifying the peripheral subunits and reassembling them to restore ATPase activity. Because of difficulties in obtaining sufficient amounts of pure components by standard biochemical procedures, we have instead tried to assemble the catalytic center from recombinant subunits where purity and quantity are not problematic. By using hybrid particles, composed of recombinant subunits and biochemically prepared subcomplexes, we have shown that subunits A, B, C, and E are necessary for ATP hydrolysis (7-10). Recently, it was determined that these four recombinant subunits could not together support ATP hydrolysis; rather, either of the biochemically isolated 14- and 15-kDa polypeptides of VC, designated polypeptides G and H, were shown to be necessary to activate ATPase activity (14).
In this study we have molecularly characterized polypeptides G and H and have determined that they encode two closely related polypeptides, which have homology to the G subunit of the proton pump of yeast (24), tobacco hornworm (23), and bovine chromaffin granule (25). Because of differences in tissue distribution, it appears that these proteins are isoforms, which we term G1 and G2. This notion is supported by the demonstration that recombinant G1 and G2 may be interchangeably used to reconstitute ATP hydrolysis. Further, the essential nature of subunit G and the finding that only the G1 isoform is detectable in several tissues together indicate that it is unlikely that both G1 and G2 are required for the in vivo function of all V-type proton pumps. At present, we are uncertain of the significance of the observed differences (Table II and Fig. 8) when G1 and G2 are used to reconstitute ATP-hydrolysis. The isolation of recombinant G1 and G2 subunits were achieved by different procedures, and it is possible that the differences observed simply occur because one isoform has been renatured and purified in a more active form. However, it is perhaps notable that similar reconstitution experiments performed with biochemically prepared polypeptides G (subunit G1) and H (subunit G2) also demonstrate reproducible differences in the reconstitution of ATPase activities, and it thus remains a possibility that in brain the isoforms interplay to produce differences in pump activities.
In addition to demonstration of isoform variability in subunit G, we have succeeded in the reconstitution of ATPase activity from entirely recombinant components, thereby providing a system to define subunit interactions within the catalytic sector.
Questions remain regarding the roles of the other peripheral components of the V pump. At present, it appears that subunit F, which is not essential for ATP hydrolysis (14), may serve to structurally link the catalytic sector to VB, the proton channel. SFD has likewise been shown to be essential for linking ATP hydrolysis to proton flow (12). Biochemical, structural, and functional data from this work clearly place subunit G within the catalytic center of the enzyme, in accord with studies performed with the V pump of tobacco hornworm (23) and in contrast to previous reports that this subunit is membrane-associated (24, 25). In this respect, it notable that the yeast phenotype resulting from the knock-out of VMA10 gene resembles that observed when other V1 (but not VO) subunits are deleted. This would further suggest that subunit G is not the V pump equivalent of subunit b of FO, as has been proposed (25). Despite this, it is likely that subunit G, like subunit F, may sit at the interface of the catalytic and transmembranous sectors and that its release from membrane components may vary depending upon the protocol used. This could account for the finding that the isolated V1 preparation from tobacco hornworm has both the G subunit and Ca2+-activated ATPase activity, whereas other preparations of V1 are inert. It will be of interest to determine where subunit G localizes by other V1 release protocols to investigate the possibility of Ca2+-activated ATPase activity in V1 preparations of other systems.
Ultimately, reconstitution of this catalytic center from purely recombinant components will allow for an understanding of the roles of subunit G and SFD in pump function and thereby define the entire catalytic requirements of the V pump of clathrin-coated vesicles.