Regulation of Tissue Factor Initiated Thrombin Generation by the Stoichiometric Inhibitors Tissue Factor Pathway Inhibitor, Antithrombin-III, and Heparin Cofactor-II*

(Received for publication, February 20, 1996, and in revised form, September 30, 1996)

Cornelis van `t Veer and Kenneth G. Mann Dagger

From the Department of Biochemistry, University of Vermont, Burlington, Vermont 05405-0068

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
Acknowledgments
REFERENCES


ABSTRACT

The effects of the stoichiometric inhibitors tissue factor pathway inhibitor (TFPI), antithrombin-III (AT-III) and heparin cofactor-II (HC-II) on thrombin generation were evaluated in a reaction system composed of coagulation factors VIIa, X, IX, VIII, and V and prothrombin initiated by tissue factor (TF) and phospholipids. Initiation of the reaction in the absence of inhibitors resulted in explosive thrombin generation for factor VIIa·TF concentrations varying from 100 to 0.25 pM with the lag time or initiation phase of thrombin generation increasing from 0 to 180 s with decreasing factor VIIa·TF concentrations. During the propagation phase, prothrombin is quantitatively activated to 1.4 µM alpha -thrombin. At normal plasma concentration (2.5 nM) full-length recombinant TFPI prolonged the initiation phase of thrombin generation 2-fold, and the rate of thrombin generation in the propagation phase of the reaction was 25-50% that of the uninhibited reaction when the reaction was initiated with 1.25-20 pM factor VIIa·TF. Inhibition of the reaction by TFPI is associated with a delay in factor V activation. In the presence of TFPI no explosive thrombin generation was observed when factor VIII was omitted from reactions initiated by factor VIIa·TF concentrations <= 20 pM. This indicates that in the presence of TFPI the factor IXa·factor VIIIa pathway becomes essential at low factor VIIa·TF concentrations. In the reconstituted system, AT-III (3.4 µM) did not prolong the initiation phase of thrombin generation when the reaction was initiated with 1.25 pM factor VIIa·TF, nor did AT-III delay factor V activation. The rate of thrombin formation in the presence of AT-III was reduced to 30% that of the uninhibited reaction, and the alpha -thrombin formed was rapidly inhibited subsequent to its generation. The addition of HC-II alone at its physiological concentration (1.38 µM) to the procoagulant mixture did not alter the rate or extent of thrombin generation. Subsequently, the thrombin formed was slowly inhibited by HC-II. The slow inactivation of thrombin by HC-II does not contribute to thrombin inhibition in the presence of AT-III. In contrast, the combination of physiological levels of AT-III and TFPI inhibited explosive thrombin generation initiated by 1.25 pM factor VIIa·TF completely. The absence of prothrombin consumption indicated that the combination of TFPI and AT-III is able to prevent the formation of prothrombinase activity at low factor VIIa·TF concentrations. The data indicate that TFPI potentiates the action of AT-III by decreasing the rate of formation and thus the amount of catalyst formed in the reaction, enabling AT-III to effectively scavenge the limited traces of factor IXa and factor Xa formed in the presence of TFPI. The initiation of thrombin generation by increasing factor VIIa·TF concentrations in the presence of physiological concentrations of TFPI and AT-III showed dramatic changes in the maximal rates of thrombin generation over small changes in initiator concentration. These data demonstrate that significant thrombin generation becomes a "threshold-limited" event with regard to the initiating factor VIIa·TF concentration in the presence of TFPI and AT-III.


INTRODUCTION

The extrinsic pathway of blood coagulation involves the activation of multiple coagulation factors leading to thrombin generation. The procoagulant reaction starts with the binding of activated factor VII (factor VIIa) to its cofactor, tissue factor (TF).1 TF is an integral membrane protein that is exposed as a result of vessel wall injury or cytokine activation of endothelial cells or peripheral blood monocytes. The membrane-bound factor VIIa·TF enzyme complex activates the zymogens factor X and factor IX by limited proteolysis (1). Factor IXa combines with factor VIIIa on the membrane surface to form a second complex that activates factor X. Once activated, factor Xa associates with factor Va on a membrane surface to form prothrombinase, which converts prothrombin into thrombin. (For reviews on blood coagulation and membrane-dependent reactions in blood coagulation see, respectively, Refs. 2 and 3). The thrombin initially formed accelerates further thrombin generation by feedback activation of the procofactors factor V and factor VIII. Thrombin may also activate factor XI (4, 5), which, in turn, activates more factor IX. Deficiencies in factors VII, X, IX, V, or VIII or prothrombin are associated with abnormal bleeding. Factor XI-deficient individuals rarely suffer from spontaneous bleeding; however, homozygotes may require replacement therapy during significant surgical challenge. Thrombin also activates platelets, which secrete their granule contents and aggregate upon activation. In addition, thrombin cleaves fibrinogen to generate the fibrin network and activates the protransglutaminase factor XIII. The fibrin-platelet aggregate, stabilized by factor XIIIa-catalyzed cross-links, forms the hemostatic plug, which maintains the integrity of the circulatory system following vessel wall perforation.

In normal hemostasis, the procoagulant system is in balance with anticoagulant systems involved in the termination of the hemostatic reaction and the fibrinolytic system, which dissolves clots once they are formed. The anticoagulant systems consist of several stoichiometric protease inhibitors, the tissue factor pathway inhibitor (TFPI), antithrombin-III (AT-III), and heparin cofactor-II (HC-II), and the dynamic protein C pathway, which involves thrombin, activated protein C, protein S, and thrombomodulin.

TFPI is a reversible, active site-directed inhibitor of factor Xa, which regulates coagulation by inhibiting factor VIIa·TF in a factor Xa-dependent manner (for a review on TFPI see Ref. 6). The TFPI·factor Xa complex binds to the factor VIIa·TF complex, resulting in the formation of a TF·factor VIIa·TFPI·factor Xa quaternary complex (7). Although no human deficiencies have been reported, the in vivo relevance of TFPI is supported by experiments that showed the sensitization of rabbits to TF-triggered disseminated intravascular coagulation after immunodepletion of TFPI (8).

AT-III is a serine protease inhibitor whose importance in hemostasis is confirmed by the association of thrombosis with heterozygous AT-III deficiency (for reviews on AT-III see Refs. 9 and 10). AT-III inhibits thrombin, factor Xa, factor IXa, factor VIIa, factor XIa, and factor XIIa in vitro by forming covalent complexes in which the active site of the protease is trapped. The action of AT-III is potentiated by heparinoids. The rate of inhibition by AT-III and the potentiation of the inhibition reaction by heparinoids varies for each target protease (10). Factor Xa is protected from inactivation by AT-III when in a membrane-associated complex with factor Va (11, 12). In contrast, factor VIIa inactivation by AT-III is only significant when the protease is bound to TF (13). The inactivation of TF-bound factor VIIa by AT-III probably involves the opening of the active site of factor VIIa upon TF binding, allowing factor VIIa inactivation by the active site-directed AT-III. The low enzymatic activity of free factor VIIa provides a mechanism by which traces of factor VIIa may circulate in blood (14).

HC-II is a serine protease inhibitor circulating in blood plasma at a concentration of ~1.2 µM (15). Thrombin is the only procoagulant reported to be inhibited by HC-II. The inhibition of thrombin by HC-II is potentiated by heparin and by dermatan sulfate (for a review on HC-II see Ref. 16). A heterozygous deficiency of HC-II (activity levels < 60%) is found in approximately 1% of the healthy population and does not appear to be a risk factor for thrombosis. A homozygous deficient individual has not yet been identified (16).

We have described reconstituted empirical (17) and mathematical (18) models for the tissue factor pathway to thrombin using purified coagulation factors and computer simulations based upon the known kinetic constants for the reactions thought to be essential in the procoagulant scheme. The reaction could be divided into two phases, an "initiation phase," in which factor V and factor VIII were quantitatively cleaved and trivial amounts of factor Xa and factor IXa were produced, and a "propagation phase" in which prothrombin was quantitatively activated (17). As the concentration of initiator (factor VIIa·TF) was reduced, the initiation phase was prolonged while the rate of thrombin generation in the propagation phase varied by only 5-fold over a 1000-fold range of factor VIIa·TF concentration. The initiation phase was shortened when the reaction was initiated in the presence of factor Va while the propagation phase was dependent upon factor VIII and factor IX at factor VIIa·TF concentrations below 100 pM. The data obtained with the reconstituted empirical model using purified coagulation factors were reasonably approximated by the mathematical model. The data presented here extend the empirical tissue factor pathway to thrombin studies to include TFPI, AT-III, and HC-II.


MATERIALS AND METHODS

Reagents

Phosphatidylserine from bovine brain, phosphatidylcholine from egg yolk and Hepes were purchased from Sigma. D-Phenylalanyl-L-arginine chloromethyl ketone (FPR-ck) and the biotinylated product were provided as gifts by Dr. R. Jenny (Haematologic Technologies Inc., Essex Junction, VT). Spectrozyme TH and Spectrozyme Xa were purchased from American Diagnostica, Inc. Q-Sepharose FF was obtained from Pharmacia Biotech Inc. All other reagents were of analytical grade. Mouse monoclonal antibodies alpha HFV-17, alpha HPC-2, and alpha HFX-10 were provided by Dr. William Church (Thrombosis Center Antibody Core Facility, Department of Biochemistry, University of Vermont).

In the experiments described, we mix together a potent mixture of enzyme and cofactor precursors together. The prevention of spontaneous activation requires extraordinary levels of purity. Contaminating enzymes at femtomolar to picomolar levels cannot be tolerated. Human coagulation factors X and IX and prothrombin were isolated from fresh frozen plasma using the methods of Bajaj et al. (19). All steps were performed at 4 °C. Factor X and factor IX were passed over a polyclonal burro anti-human prethrombin-1 antibody immunoaffinity column in 20 mM Tris, 150 mM NaCl, 1 mM benzamidine pH 7.4 (TBS/benz) and concentrated on a Q-Sepharose FF column. Traces of factor VII were removed by washing the Q-Sepharose columns with 10 mM CaCl2, 50 mM NaCl, 20 mM Tris, 1 mM benzamidine, pH 7.4. After the columns were washed with buffer without CaCl2, factor X and factor IX were eluted with TBS/Benz containing 1 M NaCl. Prothrombin was passed over anti-factor X (alpha HFX-10) and anti-protein C (alpha HPC-2) immunoaffinity columns and was concentrated and depleted of trace amounts of factor VII using Q-Sepharose as described for factor X and factor IX. Prothrombin was eluted from the Q-Sepharose column with 20 mM CaCl2, 150 mM NaCl, 20 mM Tris, 1 mM benzamidine. To inhibit traces of active enzymes in the zymogen preparations, the preparations of factor X, factor IX, and prothrombin were treated with 1 mM diisopropylphosphofluoridate at 25 °C for 1 h. The proteins were then dialyzed against 20 mM Tris, 150 mM NaCl, pH 7.4 (TBS) and treated with 10 µM FPR-ck for 1 h at 25 °C. Free FPR-ck and breakdown products were removed by extensive dialysis in TBS. The protein solutions were aliquoted and frozen in liquid nitrogen and stored at -80 °C.

Human factor V was isolated by using the methods of Nesheim et al. (20). Recombinant factor VIII and recombinant tissue factor (residues 1-242) were provided as gifts by Dr. Shu Len Liu and Dr. R. Lundblad (Hyland Division, Baxter Healthcare Corp.). Recombinant human coagulation factor VIIa was purchased from NOVO pharmaceuticals. Recombinant full-length TFPI produced in Escherichia coli was provided as a gift by Dr. K. Johnson (Chiron Corp). AT-III and HC-II were purified from barium citrate absorbed plasma by heparin-Sepharose chromatography according to the method described by Griffith et al. (21). Possible traces of heparin contamination in the AT-III preparation were removed by DEAE-cellulose chromatography (21).

Coagulation Activation Experiments

Thrombin generation initiated by factor VIIa·TF in a fully reconstituted procoagulant model was studied as described previously (17). TF was relipidated at 0.5 nM into 400 µM 75% phosphatidylcholine, 25% phosphatidylserine vesicles (PCPS), for 30 min at 37 °C in 20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH 7.4 (Hepes/Ca2+). The relipidated TF was incubated with factor VIIa for 20 min at 37 °C to allow factor VIIa·TF complex formation. Factor V and factor VIII were added to the equilibrated factor VIIa·TF mixture (total volume of addition not exceeding 0.25% of the final reaction volume), and immediately thereafter the reaction was started by the addition of a solution containing factor X, factor IX, and prothrombin, equilibrated in Hepes/Ca2+ at 37 °C for 3 min before the addition. When TFPI, AT-III, or HC-II was included it was added to the factor X, factor IX, and prothrombin mixture.

The final concentrations of the proteins in the reaction, chosen to represent mean plasma values, were 160 nM factor X, 90 nM factor IX, 0.7 nM factor VIII, 20 nM factor V, and 1.4 µM prothrombin (17). The factor VIIa concentration was varied, while TF was kept constant and in excess at 0.25 nM. The final PCPS concentration was 200 µM. Following initiation of the reaction, aliquots were withdrawn from the reaction mixture and quenched into either 20 mM EDTA/TBS, pH 7.4, to assay for thrombin formation or into 2% SDS, 0.062 M Tris, 10% glycerol, 0.04% bromphenol blue, pH 6.8, for SDS-PAGE and immunoblotting. Samples quenched in SDS were heated for 5 min at 95 °C and stored at -20 °C. Assays for thrombin activity were performed using the chromogenic substrate Spectrozyme TH. The hydrolysis of the substrate was monitored by the change in absorbance at 405 nm using a Molecular Devices Vmax spectrophotometer. Thrombin generation was calculated from a standard curve prepared by serial dilutions of alpha -thrombin. When AT-III or HC-II were added to the reaction mixture, samples were withdrawn into 20 mM EDTA/TBS containing 0.4 mM Spectrozyme TH and assayed immediately for thrombin activity. Quenching the reaction mixture in the resulting concentration of substrate stops the inactivation of thrombin by AT-III during the assay. Thrombin activity in a mixture of 125 nM alpha -thrombin and 3.4 µM AT-III was stable for 15 min in the EDTA/Spectrozyme TH mixture. Without Spectrozyme TH, alpha -thrombin was completely inactivated by AT-III under these conditions within 4 min. During the thrombin activity assay, thrombin or AT-III concentrations did not exceed 70 nM or 170 nM, respectively. These concentrations are well below those used in the control experiment, which showed the stability of thrombin in a thrombin/AT-III/Spectrozyme TH mixture, thus assuring accurate thrombin measurement in the presence of AT-III. Factor Xa generation was measured in samples quenched in a mixture of 40 mM EDTA/TBS containing excess hirudin and 0.4 mM Spectrozyme Xa. This mixture prevents further inhibition of factor Xa by AT-III as discussed above for thrombin. The hydrolysis of the substrate was measured as described for the thrombin assay. Prothrombinase concentrations were calculated from the rate of thrombin generation using a kcat of 5016 min-1 (22).

In order to follow thrombin generation via active site blotting, samples were quenched into 100 µM biotinylated FPR-ck, in TBS containing 20 mM EDTA and incubated for 20 min at 25 °C. After incorporation of the active site label, samples were further quenched in 2% SDS, 0.062 M Tris, 10% glycerol, 0.02% bromphenol blue, pH 6.8, and heated for 5 min at 95 °C.

For active site and Western blot analysis, samples were subjected to SDS-PAGE on 4-12% polyacrylamide gels under conditions described by Laemmli (23). Following SDS-PAGE, the proteins were transferred to nitrocellulose membranes for immunoblot or active site blot analysis using techniques described by Towbin et al. (24). Membranes were blocked for nonspecific binding with 5% nonfat dry milk in TBS containing 0.05% Tween and probed for prothrombin activation products using a polyclonal burro anti-human prethrombin-1 antibody (4 µg/ml) (17) or probed for factor V and the factor Va heavy chain using a murine anti-human factor V monoclonal antibody (alpha HFV-17, 10 µg/ml). The reactive bands were visualized with peroxidase-conjugated goat anti-horse IgG or peroxidase-conjugated goat anti-mouse IgG (1:5000 dilution, Southern Biotechnology Associates, Inc.) using the chemiluminescent substrate Luminol (Renaissance chemiluminescent reagent, DuPont). Kodak X-Omat film was exposed to light emitted from the hydrolysis of the added Luminol substrate. Active site blots were blocked with 0.5% Tween in TBS and stained for incorporation of biotinylated-FPR-ck using the Vectastain ABC kit (Vector Laboratories, Inc.), which employs the multiple biotin binding sites on avidin to link peroxidase-conjugated biotin to the active site-incorporated biotinylated FPR-ck. Bound peroxidase was visualized using the chemiluminescent technique as described above.


RESULTS

Effect of TFPI on Thrombin Generation by Varying Initiator Concentrations

The effect of full-length TFPI on thrombin generation initiated by the addition of factor VIIa·TF·PCPS to a mixture of prothrombin and factors X, IX, V, and VIII was studied over a wide range of initiator (factor VIIa·TF) concentrations. As previously reported, in the absence of TFPI, the reaction profile can be divided into two phases, an initiation or lag phase and a propagation phase in which prothrombin is quantitatively cleaved to thrombin (Fig. 1A). In the absence of TFPI the rate of thrombin generation in the propagation phase increased only 5-fold when the initiator concentration was varied from 0.25 to 100 pM (Fig. 1A). However, increasing the factor VIIa·TF concentration shortened the initiation phase of the reaction from 180 s at 0.25 pM initiator to almost no lag with 100 pM factor VIIa·TF. The addition of 2.5 nM TFPI (the approximate physiological concentration) resulted in extension of the initiation phase (Fig. 1B) and a reduction in the rate of thrombin generation during the propagation phase. A plot of the duration of the lag time, estimated by the intersection of the line through the linear part of the propagation phase and the x axis, versus the factor VIIa·TF concentration revealed that TFPI doubled the lag time, independent of the factor VIIa·TF concentration (Fig. 2A). However, explosive thrombin generation via ~100 pM prothrombinase still occurred during the propagation phase, even at 0.25 pM factor VIIa·TF (Fig. 2B, squares).


Fig. 1. Effect of TFPI on thrombin generation initiated with varying factor VIIa·TF concentrations. Thrombin generation is initiated by 0.25 (black-down-triangle ), 1.25 (black-diamond ), 5 (black-triangle), 20 (black-square), and 100 pM (bullet ) factor VIIa·TF. Thrombin generation curves in the absence of inhibitor are shown in A, and the thrombin generation curves in the presence of 2.5 nM TFPI are shown in B. TFPI prolongs the lag phase and reduces the rate of thrombin generation during the propagation phase, resulting in curves shifted to the right in the presence of TFPI (compare A and B).
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Fig. 2. Effect of TFPI on the lag time and the maximal rate of thrombin generation as a function of the factor VIIa·TF concentration. Lag times were estimated by the intersection of the line through the linear part of the thrombin generation during the propagation phase and the x axes. The data is obtained from the thrombin curves shown in Fig. 1. A, the lag times in minutes in the control reactions (bullet ) and in the presence of 2.5 nM TFPI (black-square). B, the maximal prothrombinase concentrations in the control reactions (bullet ) and in the presence of 2.5 nM TFPI (black-square).
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To investigate whether the effect of TFPI was a result of direct inhibition of factor Xa or the inhibition of factor VIIa·TF activity, an experiment was performed in which prothrombinase activity was initiated by the addition of various factor Xa concentrations to a mixture of 200 µM PCPS and factor IX, factor V, and prothrombin (Fig. 3). The maximum concentration of prothrombinase formed was calculated from the linear part of the thrombin generation curve. TFPI (Fig. 3, open circles) reduced prothrombinase activity by 50-65% when the reaction was initiated with either 200 or 400 pM factor Xa. At 100 pM factor Xa (Fig. 3, open circles), TFPI (2.5 nM) accounts for 90% inhibition of the rate of thrombin generation. The inhibition of prothrombinase activity became negligible when the factor Xa concentration approached the TFPI concentration (2 nM factor Xa and 2.5 nM TFPI).


Fig. 3. Effect of TFPI on thrombin generation initiated by factor Xa directly. The reactions were carried out exactly the same as in the tissue factor-initiated reactions, except that factor VIIa, tissue factor, and factor VIII were omitted. Immediately after mixing the phospholipid, cofactors, and zymogens, factor Xa was added to start the reaction. Effective prothrombinase concentrations were calculated from the observed linear thrombin generation rate. Filled circles show thrombin generation in the absence of TFPI, and open circles show the reaction in the presence of 2.5 nM TFPI. The inset shows the percentage of prothrombinase inhibition by TFPI at varying factor Xa concentrations.
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Effect of TFPI on Thrombin Generation in the Absence of Factor VIII

The influence of the factor IXa·factor VIIIa pathway on factor Xa generation in reactions initiated by factor VIIa·TF in the presence of TFPI was studied by omitting factor VIII, thus mimicking the condition of hemophilia A. The experiment performed was otherwise identical to that shown in Fig. 1. Similar results were obtained when factor IX was omitted from the mixture (results not shown). Thrombin generation curves in the absence of factor VIII are shown in Fig. 4.


Fig. 4. Effect of TFPI on thrombin generation in the absence of factor VIII, a condition that mimics hemophilia A. Thrombin generation is initiated by 0.25 (black-down-triangle ), 1.25 (black-diamond ), 5 (black-triangle), 20 (black-square), and 100 pM (bullet ) factor VIIa·TF in the absence of factor VIII. Thrombin generation curves in the absence of inhibitor are shown in A, and B shows thrombin generation in the presence of 2.5 nM TFPI. In the absence of factor VIII, thrombin generation is virtually abolished by TFPI when initiated with <= 5 pM factor VIIa·TF.
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The propagation rate is decreased in the absence of factor VIII; however, traces of factor VIIa·TF (0.25 pM) ultimately result in accumulation of significant prothrombinase (Fig. 4A, inverted triangles). Significant TFPI inhibition of thrombin formation is observed in the absence of factor VIII (Fig. 4B). In the presence of 2.5 nM TFPI, concentrations of factor VIIa·TF below 5 pM (triangles) did not result in significant thrombin generation. This implies that the explosive thrombin generation that occurs at <= 5 pM factor VIIa·TF in the presence of factor VIII in Fig. 1B is completely dependent upon the factor VIIIa·IXa pathway. At 20 pM factor VIIa·TF in the absence of factor VIII (Fig. 4B, squares) the maximum rate of thrombin generation was inhibited 93% by TFPI. The virtually linear rate of thrombin generation of 50 nM min-1 observed at 20 pM factor VIIa·TF suggests that a limited amount of factor Xa is formed before the factor VIIa·TF activity is eliminated. This rate of thrombin generation corresponds to the presence of 10 pM prothrombinase. From the data shown in Fig. 3 it follows that ~90% of the factor Xa formed is inhibited by TFPI under these conditions, indicating the formation of approximately 100 pM of factor Xa in total. The rate of thrombin generation by 20 pM factor VIIa·TF in the absence of TFPI indicates the presence of 150 pM factor Xa after 1 min. Based upon the rate of 150 pM min-1 of factor Xa generation in the absence of TFPI, a concentration of 1500 pM factor Xa is expected at 10 min if TFPI had not inhibited the factor VIIa·TF activity. Instead, only 100 pM factor Xa is produced, consistent with a 93% inhibition of factor Xa generation at 10 min. The rate of thrombin generation, however, does not significantly change after the first 2 min of the reaction, leading to the conclusion that following the initial formation of 100 pM factor Xa during the first 2 min of the reaction, TFPI completely inhibited subsequent factor Xa generation. These data provide evidence that in the reconstituted model TFPI functions by inhibiting both factor Xa and factor VIIa·TF activity. Explosive thrombin generation does occur in the presence of TFPI and the absence of factor VIII at factor VIIa·TF concentrations >= 100 pM (Fig. 4B, circles).

A comparison of the effect of TFPI on thrombin generation in the absence and presence of factor VIII reveals interesting observations regarding the influence of TFPI in hemophilia A. A plot of the maximum effective prothrombinase concentration formed during the reactions versus the initiating factor VIIa·TF concentration (Fig. 5, data obtained from Figs. 1 and 4) shows that in the hemophilia A situation (Fig. 5B) TFPI (open squares) inhibits thrombin generation at low factor VIIa·TF to less than 1% of the rate observed in its absence (filled circles). In comparison, when factor VIII is present (Fig. 5A) the effect of TFPI (open squares) on the formed prothrombinase activity is limited. These results emphasize the importance of the factor VIIIa·factor IXa pathway in overcoming TFPI-mediated inhibition of factor VIIa·TF-initiated thrombin generation and also demonstrates the potency of TFPI in inhibiting thrombin generation at low initiator concentrations.


Fig. 5. Calculated maximal effective prothrombinase concentration as a function of the factor VIIa·TF concentration in the presence of factor VIII (A) and in the absence of factor VIII (B) in the absence of inhibitor (filled circles) or with 2.5 nM TFPI (open squares). The data is calculated from the thrombin generation curves shown in Figs. 1 and 4.
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Concentration Dependence of TFPI Inhibition of Thrombin Generation

Thrombin generation initiated by factor VIIa·TF (1.25 pM) in the presence (Fig. 6A) and absence (Fig. 6B) of factor VIII was only inhibited at concentrations of TFPI >= 0.5 nM (filled squares). In the absence of the factor VIIIa·factor IX pathway (Fig. 6B), the rate of thrombin generation was decreased 80% by 1 nM TFPI (triangles), while at 2.5 nM the rate of thrombin generation was only 1% of the control value (diamonds). TFPI at 5 nM totally abolished thrombin generation in the absence of the factor VIIIa·factor IXa pathway (Fig. 6B, filled circles). When factor VIII was present, the rate of thrombin generation was inhibited 60% by 1 nM TFPI, and a slight increase in the lag time was observed (Fig. 6A, filled triangles). At 2.5 nM TFPI (diamonds), the initiation phase was doubled, when compared with the reaction in the absence of inhibitor, and the propagation rate was decreased by 70%. At 5 nM TFPI the lag time was increased from 60 to 240 s, and the rate of propagation was decreased by 90% (Fig. 6A, filled circles).


Fig. 6. Concentration-dependent inhibition of thrombin by TFPI generation initiated by 1.25 pM factor VIIa·TF in the presence (A), or absence of factor VIII (B). TFPI concentrations are as follows: 0 (open circle ), 0.5 (black-square), 1.0 (black-triangle), 2.5 (black-diamond ) and 5 nM (bullet ) TFPI.
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Comparison of the Individual and Combined Effects of TFPI and AT-III on Factor VIIa·TF-triggered Thrombin Formation

A comparative study was performed analyzing the effects of TFPI (2.5 nM) and AT-III (3.4 µM) at their respective physiological concentrations on thrombin generation initiated by traces of factor VIIa·TF. In the reconstituted procoagulant system, thrombin generation initiated by 1.25 pM factor VIIa·TF in the absence of inhibitors resulted in explosive thrombin generation after a 30-s lag time (Fig. 7, filled squares). Immunoblotting showed complete prothrombin consumption at 3 min (Fig. 8A), which was in agreement with generation of maximum thrombin activity (Fig. 7, filled squares). Immunoblotting for prothrombin fragments after SDS-PAGE under reducing conditions (Fig. 9A) showed only limited formation of prethrombin-2 (Mr 37,000) as compared with generation of the thrombin B-chain (Mr 30,000) derived from meizothrombin, meizothrombin-des-F1, and alpha -thrombin. The generation of meizothrombin and meizothrombin-des-F1 are confirmed by the generation of immunoreactive bands consistent with fragment 1.2A-chain (F1.2A) (Mr 47,000) of meizothrombin and fragment 2A-chain (F2A) (Mr 18,000), the latter formed by autoproteolysis of meizothrombin at Arg156 (25), leading to the release of fragment 1 and the formation of meizothrombin-des-F1. These data, together with the lack of prethrombin-2, indicate that thrombin generation proceeded primarily by cleavage of prothrombin at Arg320, forming meizothrombin, followed by cleavage at Arg271, resulting in the formation of alpha -thrombin. Accordingly, active site blotting using biotinylated-FPR-ck (Fig. 10A) shows formation of the meizothrombin-derived meizothrombin-des-F1. Films exposed for a longer interval demonstrate the initial generation of meizothrombin (not shown). Prothrombin is also subject to cleavage by the various thrombins at Arg156 (25). This results in the formation of prethrombin-1 that lacks the GLA domain containing fragment 1. The inability to interact with a membrane via the GLA domain results in reduced rates of activation of prethrombin-1 and meizothrombin-des-F1 by prothrombinase.


Fig. 7. Comparison of the effect of physiological TFPI and AT-III concentrations on factor VIIa·TF (1.25 pM) initiated thrombin generation. Immunoblotting analyses for prothrombin and factor V products of this experiment are shown in Figs. 8, 9, and 11. Thrombin active site blots of this experiment are shown in Fig. 10. The reactions are control reaction in the absence of inhibitors (black-square), in the presence of 2.5 nM TFPI (open circle ), in the presence of 3.4 µM AT-III (square ), and in the presence of the combination 3.4 µM AT-III and 2.5 nM TFPI (diamond ).
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Fig. 8. Effect of TFPI and AT-III on prothrombin activation as evaluated by immunoblotting of SDS-quenched samples of the experiment described in Fig. 7. Reaction time is indicated in minutes above the gel lanes. A, control without inhibitors; B, 2.5 nM TFPI; C, 3.4 µM AT-III; D, 3.4 µM AT-III, 2.5 nM TFPI. II, prothrombin (Mr 72,000); mIIa des-F1, meizothrombin-des-fragment 1 (Mr 50,000); Pre-1, prethrombin-1 (Mr 50,000); IIa, alpha -thrombin (Mr 38,500); F1.2, fragment 1·2 (Mr 37,000); F2, fragment 2 (Mr 14,000), TAT, thrombin·AT-III complex (Mr 97,000).
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Fig. 9. Immunoblot of prothrombin activation after SDS-PAGE under reducing conditions of the experiment shown in Fig. 7. Reaction time is indicated in minutes above the gel lanes. A, control without inhibitors; B, 2.5 nM TFPI; C, 3.4 µM AT-III; D, 3.4 µM AT-III, 2.5 nM TFPI. II, prothrombin (Mr 76,000); Pre-1, prethrombin-1 (Mr 50,000); F1·2A, fragment 1·2A-chain (Mr 47,000); B-chain, thrombin B-chain (Mr 30,000); F1·2, fragment 1·2 (Mr 40,000); Pre-2, prethrombin-2 (Mr 37,000); F2A, fragment 2A-chain (Mr 18,000); F2, fragment 2 (Mr 14,000); TAT, thrombin·AT-III complex (Mr 85,000).
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Fig. 10. Active site blot of the experiment described in Fig. 7 using biotinylated-FPR-ck showing the thrombin species with an active site at different times in the reaction. Reaction time is indicated in minutes above the gel lanes. A, control without inhibitors; B, 2.5 nM TFPI; C, 3.4 µM AT-III; D, 3.4 µM AT-III, 2.5 nM TFPI. mIIa, meizothrombin (Mr 72,000); mIIa des-F1, meizothrombin-des-fragment 1 (Mr 50,000); IIa, alpha -thrombin (Mr 38,500).
[View Larger Version of this Image (55K GIF file)]


The addition of 2.5 nM TFPI (Fig. 7, open circles) to the reaction increased the initiation phase from 60 to 150 s and reduced the rate of thrombin generation in the propagation phase by 70%. The point of total prothrombin consumption was delayed to 10 min as shown by immunoblotting (Fig. 8B), which is consistent with the thrombin activity data. TFPI did not seem to change the thrombin generation pathway, since meizothrombin products prevail on the reduced immunoblot and on the nonreduced active site blot in the presence of TFPI (Figs. 9B and 10B). Maximum thrombin activity is reached (Fig. 7) when a considerable amount of the Mr 50,000 component is present in the reaction (Fig. 8B, 10 min). These data suggest that the majority of the Mr 50,000 intermediate is meizothrombin-des-F1, which expresses activity toward the synthetic substrate in the thrombin activity assay.

The addition of 3.4 µM AT-III (Fig. 7, open squares) to the reaction in the absence of TFPI did not significantly influence the lag time of thrombin generation. However, AT-III reduced the rate of appearance of active thrombin by 70%. Thrombin activity reached a peak of 600 nM after 3 min, followed by a decline of thrombin activity with an observed t1/2 of approximately 60 s. At 10 min, essentially all thrombin activity was inhibited. Immunoblotting for prothrombin products showed that AT-III reduced the rate of prothrombin consumption by ~50%, resulting in a slight delay in prothrombin consumption compared with the control reaction (Fig. 8, compare A and C). After the appearance of thrombin activity a Mr 97,000 immunoreactive band appeared, indicating formation of an alpha -thrombin·AT-III complex. Upon longer exposure of the immunoblot, a reactive band was also detected at Mr 120,000, indicating the formation of a meizothrombin-des-F1·AT-III complex. The increase in intensity of the alpha -thrombin·AT-III band parallels the decrease in thrombin activity. Similarly, the transient incorporation of biotinylated FPR-ck into the different forms of thrombin (Fig. 10C) also parallels the peak of thrombin activity (Fig. 7, open squares). In general, the formation of thrombin·AT-III complexes paralleled the decline in thrombin activity; however, a significant amount of noncomplexed, free thrombin B-chain is observed in the immunoblot after 20 min (Fig. 9C). No thrombin activity is detectable at this stage of the reaction, indicating that this thrombin, while inhibited by AT-III during the reaction, is present as an SDS-reversible thrombin·AT-III complex.

Thrombin generation was nearly abolished by the combination of 2.5 nM TFPI and 3.4 µM AT-III when the reaction was initiated by 1.25 pM factor VIIa·TF (Fig. 7, open diamonds). However, some active thrombin was still generated at a rate of 1.4 nM min-1. A maximum of 14 nM thrombin was reached at 10 min, after which a stable level of thrombin was maintained for up to 15 min. This was followed by a slow decline in thrombin activity so that after 20 min 11 nM thrombin was still present. Significant prothrombin consumption was not observed, and densitometry of the prothrombin band showed no significant decrease over the 20-min period evaluated (Fig. 8B). Meizothrombin-des-F1 and prethrombin-1 were formed in time, and on longer exposures of the immunoblot a Mr 120,000 reactive band is observed, indicating the formation of meizothrombin-des-F1·AT-III complexes. Consistent with this is the presence of prothrombin fragment 2-A observed starting at 7 min on the reduced immunoblot (Fig. 9D). The relatively stable activity of the thrombin formed under these conditions seems to be the result of the activation of prothrombin and the inactivation of thrombin occurring at similar rates, producing a thrombin steady state.

TFPI and AT-III when added individually decrease the maximum rate of thrombin generation by 70%. The thrombin generation rate in the presence of both TFPI and AT-III was expected to be 10% of the control, or 100 nM/min, if the effects of TFPI and AT-III were multiplicative. However, the observed thrombin generation rate was 1.4 nM/min in the presence of both TFPI and AT-III. This result indicates that TFPI and AT-III together are ~70-fold more potent at inhibiting the reaction than the inhibitors acting separately. Thus, the two inhibitors, acting in concert, seem to provide a synergistic inhibitory effect.

In the absence of inhibitors, factor V activation is a major event occurring during the initiation phase of the reaction (17). The effects of TFPI and AT-III on factor V activation were studied by immunoblotting with monoclonal alpha HFV-17, which recognizes intact factor V and the factor Va heavy chain (residues 307-506). In the control experiment and in the presence of AT-III, factor V was partially activated at 30 s (Fig. 11A and C). At 60 s, complete cleavage of factor V had occurred, indicated by the disappearance of single chain factor V (Mr 330,000) and the appearance of the heavy chain of factor Va (Mr 105,000) (Fig. 11, A and C). When TFPI was added, factor V cleavage was delayed until 60 s but complete at 120 s (Fig. 11B). The activation of factor V in reactions with the combination of AT-III and TFPI was only slightly slower than that observed with TFPI alone (Fig. 11D). These data suggest that factor V is a preferred substrate for thrombin in the reaction, since minimal amounts of thrombin produced during the initiation phase are still able to quantitatively generate the heavy chain.


Fig. 11. Effect of AT-III and TFPI on the activation of factor V during the reaction as followed by the disappearance of intact factor V (FV, Mr 330,000) and appearance of the factor Va heavy chain (HC, Mr 105,000) by immunoblotting with monoclonal antibody alpha HFV-17 as described under "Materials and Methods." Reaction time is indicated in minutes above the gel lanes. Samples of the experiment shown in Fig. 7 were withdrawn and quenched in SDS. A, control without inhibitors; B, 2.5 nM TFPI; C, 3.4 µM AT-III; D, 3.4 µM AT-III, 2.5 nM TFPI.
[View Larger Version of this Image (79K GIF file)]


Effect of HC-II on Thrombin Generation

The experiment shown in Fig. 12 compares the effects of HC-II and AT-III, at their respective physiological concentrations, on thrombin formation. HC-II (1.38 µM) does not influence thrombin generation initiated by 1.25 pM factor VIIa·TF (Fig. 12, filled squares). Subsequently, a slow decline in the thrombin concentration is observed. AT-III (3.4 µM) inhibits the rate of thrombin formation, and in contrast to the HC-II case, the subsequent decrease in thrombin activity is much faster (open squares). The combination of AT-III and HC-II resulted in a thrombin generation curve identical to that observed with AT-III alone (Fig. 12, diamonds), indicating that the contribution of HC-II to thrombin inhibition is insignificant in the presence of the faster acting AT-III.


Fig. 12. Effect of HC-II on thrombin generation compared and combined with AT-III. Thrombin generation was initiated with 1.25 pM factor VIIa·TF. open circle , control; black-square, 1.38 µM HC-II; square , 3.4 µM AT-III; black-diamond , 1.38 µM HC-II, 3.4 µM AT-III.
[View Larger Version of this Image (17K GIF file)]


The Dependence of Thrombin Formation on the Factor VIIa·TF Concentration in the Presence of both TFPI and AT-III

The influence of varying factor VIIa·TF concentrations on thrombin generation in the presence of both TFPI and AT-III is shown in Fig. 13. Thrombin generation occurred slowly when initiated with 10 pM or lower concentrations of factor VIIa·TF (Fig. 13, triangles). Explosive thrombin generation was observed at 25 pM or higher concentrations of factor VIIa·TF (Fig. 13, squares). A plot of the maximal rate of thrombin generation as a function of the factor VIIa·TF concentration (based on the data shown in Fig. 13) is shown in Fig. 14A. The dramatic increase in the maximal rate of thrombin generation following initiation by factor VIIa·TF concentrations >10 pM defines a factor VIIa·TF concentration threshold for explosive thrombin generation. This observation is in marked contrast to that observed in the absence of inhibitors in which only the initial phase of the reaction was sensitive to factor VIIa·TF. These results clearly demonstrate the threshold-limited nature of the reconstituted model in the presence of TFPI and AT-III. This threshold in the factor VIIa·TF concentration curve defines a "go" or "no go" switch for the blood coagulation reaction. When the threshold (10-20 pM) is overcome, the amount of thrombin made available over the first 10 min is virtually independent of the initiator (factor VIIa·TF) concentration (Fig. 14B).


Fig. 13. Thrombin generation by varying factor VIIa·TF concentrations in the presence of 3.4 µM AT-III and 2.5 nM TFPI. Initiating factor VIIa·TF concentrations are 5 (black-diamond ), 10 (black-triangle), 25 (black-square), and 125 pM (bullet ). Explosive thrombin generation initiated by factor VIIa·TF becomes a threshold event in the presence of AT-III and TFPI (compare 10 pM and 25 pM factor VIIa·TF).
[View Larger Version of this Image (16K GIF file)]



Fig. 14. The maximal rate of thrombin formation in the presence of both AT-III (3.4 µM) and TFPI (2.5 nM) is shown in A as a function of the factor VIIa·TF concentration. The integrated area under the thrombin formation curves over the first 10 min of the reaction is shown in B. The data are obtained from the thrombin curves shown in Fig. 13.
[View Larger Version of this Image (16K GIF file)]


From the experiments shown in Figs. 7, 8, 9, 10, 11 and 13 it follows that AT-III must have an effect on factor Xa generation. An illustration of this is shown in Fig. 15. The uninhibited factor Xa generation rate (open circles) is decreased by 50% in the presence of AT-III (open squares) during the initial 3 min of the reaction. However, after 3 min the rates of factor Xa generation become equal regardless of the presence of the inhibitor. This result suggests that the ability of AT-III to inhibit factor Xa is restricted to the early beginning of the reaction. This observation is consistent with the conclusion that factor Va protects factor Xa from inactivation by AT-III (11, 12). Thus, subsequent to its activation, factor Va reduces the potential for AT-III to inhibit factor Xa. The rate of factor Xa formation at the later stages of the reaction is probably limited by the concentration of active factor VIIIa in the reaction. Factor VIIIa activity is only transiently present because of the dissociation of its polypeptide chains (26-28) and/or proteolytic inactivation (29-33). The observation that the ability of AT-III to inhibit factor Xa generation is limited to the initial stage of the reaction is of major importance with respect to the proposed synergistic action of AT-III and TFPI. TFPI significantly delays factor V activation, thereby increasing the ability of AT-III to inhibit factor Xa.


Fig. 15. Effect of AT-III on factor Xa generation. Thrombin generation (filled symbols) and factor Xa generation (open symbols) were initiated by 5 pM factor VIIa·TF in the absence (circles) or presence (squares) of AT-III (3.4 µM) and measured as described under "Materials and Methods."
[View Larger Version of this Image (17K GIF file)]



DISCUSSION

TFPI is a major inhibitor of thrombin generation, extending the "lag" time, or initiation phase, of thrombin generation and reducing the rate of thrombin generation during the propagation phase. The delay in the propagation phase of thrombin generation shows that TFPI exerts a relatively rapid inhibitory effect on the reaction, which is associated with delayed factor V activation. In the presence of TFPI as the only inhibitor, the factor IXa·factor VIIIa complex ultimately generates sufficient factor Xa, resulting in a bolus of prothrombinase activity independent of the initiator concentration. In the absence of the factor IXa·factor VIIIa pathway, TFPI reduces the maximally formed prothrombinase activity to 1% of that observed in the absence of the inhibitor at low (<= 5 pM) factor VIIa·TF concentrations. Thus, the propagation phase of thrombin generation in the presence of TFPI is totally dependent upon factor IXa·factor VIIIa activity at low concentrations of factor VIIa·TF. These results provide quantitative support for the hypothesis that the failure of the hemostatic response upon injury in patients with hemophilia A or B is, in part, caused by the inactivation of low concentrations of factor VIIa·TF by TFPI (34). In flow studies using purified proteins Repke et al. (35) showed that in the presence of TFPI, ongoing factor Xa generation by factor VIIa·TF was only obtained in the presence of the factor IXa·factor VIIIa pathway.

Several groups have reported the rate constants for the inhibition of factor Xa by full-length TFPI (36-38), and a recent study by Jesty et al. (39) reports the rate constants for the inhibition of the factor VIIa·TF complex by the preformed factor Xa·TFPI complex. The complexity of the procoagulant reactions (no steady state conditions established and enzyme occupation by multiple substrates) combined with the complexity of the inhibition reactions by TFPI makes it difficult to determine whether the observed inhibition of the reaction by TFPI can be explained by the reported kinetic constants. Significant (~90%) inhibition of factor VIIa·TF activity by TFPI·factor Xa was observed by Jesty et al. (39) after 60 s. This rapid effect of TFPI on factor VIIa·TF is in agreement with the rapid effect of TFPI on the initiation of the reaction in the reconstituted model.

AT-III inhibits serine proteases by trapping the enzyme at an intermediate stage of proteolysis of the Arg393-Ser394 peptide bond (40-42). Speculation based upon the relatively high M) physiological concentrations of AT-III compared with the trace amounts (2.5 nM) of TFPI in the circulation have suggested a potential role for AT-III as an inhibitor of factor VIIa·TF. However, no empirical comparisons on the relative potencies of TFPI and AT-III as inhibitors of factor VIIa·TF-dependent thrombin generation have been reported. The inhibitor AT-III displays a completely different profile of inhibition compared with TFPI. At 1.25 pM factor VIIa·TF, no increase in lag time is observed, while the rate of prothrombin consumption during the propagation phase was reduced by 50% in the presence of physiological concentrations of AT-III. These data demonstrate that full-length TFPI is the major inhibitor of factor VIIa·TF-initiated thrombin formation, while AT-III has no significant influence upon the initiation phase of the reaction, which is almost totally a function of the factor VIIa·TF concentration. The inhibition of factor VIIa bound to TF by AT-III (13) is too slow to significantly inhibit the reaction during its early phase.

In experiments initiated with 5 pM factor VIIa·TF, AT-III inhibited factor Xa generation by 50% during the initiation phase. This is consistent with a slight decrease in prothrombin consumption observed in the presence of AT-III. However, the reduced amounts of factor Xa and factor IXa generated by factor VIIa·TF when TFPI is present seem to become efficiently scavenged by AT-III, thus preventing explosive thrombin generation (Fig. 7, open diamonds).

Factor Va has been reported to protect factor Xa from inactivation by AT-III (11, 12). Factor V is cleaved by 2 min in the reaction and the picomolar amounts of factor Xa generated contemporaneously are saturated by this excess of factor Va and thus relatively protected against inactivation by AT-III. However, factor Xa inactivation can occur prior to factor V activation and/or prothrombinase complex formation. In this respect, the observed delay of factor V activation in the presence of TFPI may play an important role in the potentiation of the action of AT-III by TFPI (Fig. 7).

In the absence of TFPI, protection of factor IXa by factor VIIIa against inactivation by AT-III is probably of less importance because of the relatively low concentration and the transient presence of active factor VIIIa due to its dissociation (26-28) and/or proteolytic inactivation (29-33). In contrast to prothrombinase, the amount of factor VIIIa cofactor is probably the limiting factor for the formation of the factor IXa·factor VIIIa complex. In the presence of TFPI, however, the observed inhibitory effect of AT-III is probably also due to scavenging of the trace amounts of factor IXa generated. In this model the threshold for explosive thrombin generation in the presence of 2.5 nM TFPI and 3.4 µM AT-III is ~20 pM factor VIIa·TF. The threshold level coincides with the minimal factor VIIa·TF concentration (20 pM) needed to generate considerable thrombin in the presence of TFPI and the absence of the factor IXa·VIIIa pathway (Fig. 4). This is an indication that AT-III prevents explosive thrombin generation by inhibiting the factor IXa·VIIIa pathway. This coincidence may, however, also be the result of a change in the reaction in the range of 10-20 pM from a reaction with no lag time to a reaction with a considerable lag time, which allows the effects of the additional factor Xa generation by factor IXa·factor VIIIa and the effects of the inhibitors to become more prominent. Overall, the effects of TFPI and AT-III acting in concert seem to result in a synergistic inhibition of thrombin generation at low concentrations of initiator, leading to a 70-fold higher inhibition than expected if their combined action would have been multiplicative. Although it seems that TFPI and AT-III act in synergy under these conditions, such claims of synergism must be made with caution without full knowledge of the mechanisms involved (43). At present the complexity of the reaction does not allow a proof of true synergism between TFPI and AT-III.

The major inhibitory effect of TFPI on factor VIIa·TF-initiated thrombin generation in the reconstituted model predicts that a TFPI deficiency would be a major risk factor for thrombosis. Titration of the effect of TFPI on thrombin generation revealed that TFPI exerts a significant inhibitory effect at 1 nM (Fig. 6). This TFPI concentration is approximately 50% of the normal plasma concentration, suggesting that an individual with a 50% TFPI level would derive a significant inhibitory benefit. Our data suggest, however, that a homozygous TFPI deficiency would result in massive thrombin formation, which may not be compatible with life. However this hypothesis does not take into account that the dependence on TFPI of inhibition of factor VIIa·TF-initiated thrombin generation might be less in the presence of the dynamic protein C pathway.

Experiments with physiological concentrations of HC-II (1.38 µM) predict an insignificant role for this inhibitor as compared with AT-III. Thrombin generation proceeds in a similar fashion whether or not HC-II is present. Although a marginal effect of HC-II was observed on the activity of thrombin after the quantitative activation of prothrombin, no additional thrombin-inhibitory potential was observed when HC-II was combined with physiological concentrations of AT-III. This result supports the hypothesis that HC-II is not important as a coagulation inhibitor and is in agreement with the lack of a thrombotic tendency in individuals with reduced HC-II levels (16).

The combined effects of TFPI and AT-III prevent explosive thrombin generation by traces of factor VIIa·TF in the fully reconstituted system. The dramatic change in the rate of thrombin generation over a small change in the initiating factor VIIa·TF concentration (Figs. 13 and 14) clearly demonstrates that significant thrombin generation becomes a threshold-limited event with regard to the initiating factor VIIa·TF concentration in the presence of TFPI and AT-III. The presence of traces of fibrinopeptide A, prothrombin F1·2, and thrombin·AT-III complexes in the plasma of normal individuals indicates that very low, but constant triggering of the coagulation cascade occurs in the unperturbed circulation. The present data suggest that the low level of basal activation of the coagulation system is largely controlled by the combined action of inhibitors like TFPI and AT-III, which prevent this apparent basal activity from turning into massive thrombin formation.


FOOTNOTES

*   This work was supported by National Institutes of Health Grant P01HL46703 (to K. G. M.) and a TALENT-stipendium from the Netherlands Organization for Scientific Research (to C. V. V). The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Portions of this work were presented at the 37th annual meeting of the American Society of Hematology, December 1-5, 1995, Seattle, WA (44).


Dagger    To whom correspondence should be addressed.
1    The abbreviations used are: TF, tissue factor; TFPI, tissue factor pathway inhibitor; AT-III, antithrombin-III; HC-II, heparin cofactor-II; FPR-ck, D-phenylalanyl-L-arginine chloromethyl ketone; TBS, Tris-buffered saline; PAGE, polyacrylamide gel electrophoresis; F1, fragment 1; F2, fragment 2; IIa, alpha -thrombin.

Acknowledgments

We thank Dr. Kirk Johnson (Chiron Corp.) for the generous gift of recombinant TFPI; Dr. Shu Len Liu and Dr. Roger Lundblad (Hyland Division, Baxter Healthcare Corp.) for providing us with recombinant factor VIII and recombinant tissue factor; Dr. William Church (Antibody Core, Department of Biochemistry, University of Vermont) for providing the monoclonal antibodies; Dr. Richard Jenny (Haematologic Technologies) for providing FPR-ck; and Dr. Michael Kalafatis for critical reading of the manuscript. We thank Kelly Begin and Neal Golden for technical assistance.


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