Generation of Alzheimer Amyloid beta  Peptide through Nonspecific Proteolysis*

(Received for publication, August 22, 1996)

Lars O. Tjernberg Dagger §, Jan Näslund Dagger , Johan Thyberg par , Samuel E. Gandy **, Lars Terenius Dagger and Christer Nordstedt Dagger Dagger Dagger

From the Dagger  Laboratory of Biochemistry and Molecular Pharmacology, Section of Experimental Alcohol and Drug Addiction Research, Department of Clinical Neuroscience, and par  Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institute, S-171 77 Stockholm, Sweden and ** Department of Neurology and Neuroscience, Cornell University Medical College, New York, New York 10021

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
REFERENCES


ABSTRACT

Polymerization of Alzheimer amyloid beta  peptide (Abeta ) into amyloid fibrils is associated with resistance to proteolysis and tissue deposition. Here, it was investigated whether Abeta might be generated as a protease-resistant core from a polymerized precursor. A 100-amino acid C-terminal fragment of the Alzheimer beta -amyloid precursor protein (C100), containing the Abeta and cytoplasmic domains, polymerized both when inserted into membranes and after purification. When subjected to digestion using the nonspecific enzyme proteinase K, the cytoplasmic domain of C100 was degraded, whereas the Abeta domain remained intact. In contrast, dissociated C100 polymers were almost completely degraded by proteinase K. Mammalian cells transfected with the human Alzheimer beta -amyloid precursor gene contained a fragment corresponding to C100, which needed similar harsh conditions to be dissolved, as did polymers formed by purified C100. Hence, it was concluded that C100 polymers are formed in mammalian cells. These results suggest that the C terminus of Abeta can be generated by nonspecific proteases, acting on a polymerized substrate, rather than a specific gamma -secretase. This offers an explanation of how the Abeta peptide can be formed in organelles containing proteases capable of cleaving most peptide bonds.


INTRODUCTION

The Alzheimer amyloid beta  peptide (Abeta )1 is the primary constituent of the amyloid deposited in the brain parenchyma and blood vessels of the brain in association with Alzheimer's disease (AD) (1-4). Abeta is generated through proteolytic processing of Alzheimer beta -amyloid precursor protein (beta -APP), a transmembrane protein expressed in most mammalian cells (5). Much interest has been focused on the metabolic mechanisms generating Abeta (6). beta -APP can be metabolically processed by at least two pathways. The alpha -secretase pathway cleaves beta -APP in the central section of the Abeta domain (7, 8) and thereby precludes amyloidogenesis, whereas the beta -secretase pathway generates the free N terminus of intact Abeta (6). The C-terminal beta -APP fragment containing the intact Abeta and cytoplasmic domains will hereafter be referred to as C100 (9). Subsequently, both pathways converge, and the beta -APP metabolites are cleaved by a protease activity, tentatively named gamma -secretase, generating the free C terminus of Abeta and a related, nonamyloidogenic fragment, p3 (10, 11). The enzyme or enzymes catalyzing gamma -secretase cleavage have not yet been identified. The enzymatic characteristics of gamma -secretase activity include a certain degree of nonspecificity, since the beta -APP fragment can be cleaved after either amino acid 40 or amino acid 42 of the Abeta domain (6). In either case, the cleavage site is located within the predicted transmembrane region of beta -APP (5) and should therefore be protected by the phospholipid bilayer, raising the possibility that this cleavage may not occur on the C100 substrate as long as it is membrane-inserted.

Several studies suggest that gamma -secretase activity is localized to an endosomal or lysosomal compartment (12-14). Lysosomes contain a multitude of proteolytic enzymes (15) and therefore it seems unlikely that C100 in endosomes or lysosomes will encounter protease activities with defined and narrow substrate specificities (i.e. a specific gamma -secretase). Instead, C100 will be present in an environment of enzymes cleaving many or most peptide bonds. In an earlier article, it was reported that Abeta acquires protease resistance in association with polymerization (16). It is therefore possible that the Abeta domains of several C100 molecules can interact and generate a "protease-resistant core," capable of withstanding the nonspecific proteolytic environment of endosomes or lysosomes. In the present study we have investigated whether purified and membrane-associated C100 polymers can serve as a substrate for the generation of Abeta by nonspecific proteolysis, using recombinant C100 and proteases with various substrate specificities.


EXPERIMENTAL PROCEDURES

Expression of C100 in Insect Cells

Sf9 cells were grown in Grace's supplemented insect medium with 10% fetal bovine serum (Life Technologies, Inc.) to a density of 106 cells/ml (in a total volume of 500 ml in a 1,000-ml spinner flask). The cells were then infected with recombinant baculovirus containing the C100 gene (generously provided by Dr. Rachael Neve, Harvard University. Four days after infection, the cells were centrifuged at 1,000 × g for 10 min. The pellet was washed with phosphate buffer, pH 6.2, containing 1 mM phenylmethylsulfonyl fluoride (PMSF) and centrifuged again. The harvested cells were stored at -80 °C in aliquots of 108 cells/vial.

Gradient Centrifugation of Infected Cells

Infected and harvested cells (one vial, 108 cells) were homogenized in a Dounce homogenizer with 2 ml of 5 mM Hepes buffer, pH 7.4, containing 1 mM PMSF. The homogenate was centrifuged at 500 × g for 5 min. The supernatant was loaded on a 10-30% sucrose gradient with 5 mM Hepes, pH 7.4, and centrifuged in a swinging bucket rotor (Beckman SW 40 Ti) at 200,000 × g for 16 h. Twelve fractions, 800 µl each, were aspirated from the top of the tubing. A 100-µl aliquot of each fraction was subjected to SDS-PAGE and immunoblotting.

Purification of C100

Infected and harvested cells (two vials, 2 × 108 cells) were homogenized in a Dounce homogenizer with 2 ml of 5 mM Hepes buffer containing 1 mM PMSF. The homogenate was centrifuged at 20,000 × g for 10 min. The pellet was triturated in 1 ml of 5 mM Hepes buffer with 1 mM PMSF. A 23% sucrose/5 mM Hepes solution was underlaid the homogenized pellet. Centrifugation was performed at 125,000 × g for 1.5 h in a fixed angle rotor (Beckman TLA 45). The resulting pellet was extracted with 300 µl of methanol/200 µl of chloroform for 1 h. After addition of 100 µl of methanol, the sample was centrifuged for 30 min at 125,000 × g. The pellet was extracted overnight with 200 µl of 70% formic acid with continuous stirring. The sample was centrifuged for 30 min at 125,000 × g, and the supernatant was injected on a Superose 12 size exclusion column and eluted with 70% formic acid. Fractions were collected and analyzed by SDS-PAGE and immunoblotting using 6E10 and 369 as primary antibodies. The fractions with the highest content of C100 were pooled and aliquoted prior to evaporation of the mobile phase in a vacuum centrifuge.

Digestion of Purified C100 with Proteases

Purified C100 was incubated in Tris-buffered saline (TBS) containing 150 mM NaCl and 50 mM Tris-HCl, pH 7.4, at 37 °C for 96 h. The incubated fractions were digested with 0, 10, 100, or 1000 µg of proteinase K/ml for 5 h. Purified C100 was also incubated for 0, 4, 48, and 170 h prior to digestion with proteinase K. In the same experiment, polymerized C100 (48 h incubation) was dissolved in 70% formic acid with continuous stirring for 2 h, lyophilized, and digested with proteinase K (100 µg/ml). The polymerized and digested samples were centrifuged at 22,000 × g, and 170 µl of the supernatant was replaced with an equal volume of 2 mM PMSF, M urea, and 50 mM Tris, pH 10. After 3 days with continuous stirring, the samples were analyzed by SDS-PAGE and immunoblotting.

Digestion of Membrane-bound C100 with Proteases

The fractions from the gradient centrifugation with the highest C100 content, corresponding to 23-30% sucrose, were diluted with TBS, pH 7.4, and centrifuged at 100,000 × g for 1 h to remove the sucrose. The pellet was suspended in the same buffer, aliquoted, and incubated at 37 °C in a shaking water bath overnight. Two volumes of either trypsin (150 µg/ml in 50 mM ammonium bicarbonate buffer, pH 7.8), or proteinase K (50 µg/ml in 50 mM TBS, pH 7.4) were added to the samples. After 5 h at 37 °C, the samples were analyzed by SDS-PAGE and immunoblotting.

Digestion of the Cytoplasmic Domain of C100 with Proteases

A synthetic peptide corresponding to amino acids 48-99 in C100 was a gift from Dr. Andrew J. Czernik (Rockefeller University). The peptide was incubated at 100 µM in TBS, pH 7.4, for 1 week. Proteinase K was added to final a concentration of 50 µg/ml. After 5 h at 37 °C, the samples were analyzed by SDS-PAGE and immunoblotting.

SDS-PAGE and Immunoblotting

Gradient gels, 5-18%, were run in a Tris-Tricine buffer system (17). Laemmli sample buffer containing 6 M urea was added to the samples. After boiling for 5 min, 80 µl of sample was applied to the gel. After 3.5 h at 70 mA, the gel was either stained with Coomassie Brilliant Blue or electroblotted overnight to a nitrocellulose membrane (0.2-µm pore size, Schleicher & Schuell). The membranes were incubated with monoclonal antibodies 6E10 and 4G8 (mouse), directed against residues 5-10 and 18-21 in Abeta , respectively, and polyclonal antibody 369 (rabbit), recognizing epitopes located in the C terminus of C100.2 Horseradish peroxidase-coupled secondary antibodies and chemiluminescence detection (Amersham) were used to visualize bound primary antibodies.

Electron Microscopy

To determine the intracellular localization of C100, infected cells were fixed in 2% formaldehyde/0.1% glutaraldehyde in phosphate-buffered saline, pH 7.3, dehydrated in graded ethanol, and embedded in LR White. Thin sections were immunogold stained with 369 as primary antibody and anti-rabbit IgG conjugated to 10 nm colloidal gold particles as secondary antibody. Purified C100 was incubated (96 h) as above. One sample was digested with proteinase K (50 µg/ml) for 5 h, and one was not digested. The samples were centrifuged for 1 h at 125,000 × g, and the pellet was triturated in water (Milli-Q, Waters). A 5-µl sample of this suspension was placed on a grid covered by a carbon-stabilized Formvar film. After 0.5-1 min, excess fluid was adsorbed on a filter paper, and the grids were air dried. Negative staining was made with 2% uranyl acetate in water. For immunostaining, the grids were incubated on droplets of Tris-buffered saline, pH 7.4, containing 0.1% bovine serum albumin to block nonspecific binding. The samples were then exposed to primary antibodies 6E10 and 369, washed, and exposed to secondary antibodies conjugated to colloidal gold particles of different sizes (anti-mouse IgG conjugated to 10 nm of colloidal gold particles and anti-rabbit IgG conjugated to 20 nm of colloidal gold particles). Both primary and secondary antibodies were dissolved in TBS, pH 7.4, with 0.1% bovine serum albumin, which also was used to wash the samples after exposure to antibodies.

Extraction of C100 from Chinese Hamster Ovary (CHO) Cells

CHO cells were stably transfected with beta -APP751SW (18). Approximately 40 × 106 cells were homogenized in a Dounce homogenizer with 1 ml of 0.3 M sucrose in 20 mM Tris, pH 7.3. The homogenate was centrifuged for 5 min at 500 × g. The supernatant was centrifuged for 1.5 h at 125,000 × g in a Beckman TLA rotor. The resulting pellet was suspended in 1% SDS in 20 mM Tris, 7.4, and the centrifugation was repeated. The supernatant was lyophilized, and the pellet was delipidated with methanol/chloroform. A solution of 9 M urea/100 mM Tris, pH 10, was added to the pellet. After 1 day with continuous stirring, Laemmli sample buffer was added to the sample. The lyophilized SDS extract was dissolved in Laemmli sample buffer supplemented with urea. The samples were then subjected to SDS-PAGE followed by immunoblot analysis with 6E10 or 369 as the primary antibody.


RESULTS

Proteolytic Digestion of Polymerized C100 Yields Peptides Indistinguishable from Synthetic Abeta

Previously, it was shown that polymerization of synthetic Abeta into fibrils leads to resistance to proteolytic degradation (16). We therefore investigated whether purified C100, which readily polymerizes and forms fibrils (9, 28), also develops protease resistance in association with fibril formation.

Purified C100 was allowed to polymerize by incubation in TBS for 96 h before the addition of proteinase K, an enzyme capable of cleaving most peptide bonds (19). The samples were then subjected to SDS-PAGE, followed by immunoblot analysis using 6E10 or 369 as primary antibody. The 6E10 epitope is located in the N terminus (amino acids 5-10), and the 369 epitopes are located in the cytoplasmic domain (amino acids 70-75 and 90-95) of C100. Polymerized C100 could not be completely dissolved under the conditions used, although the bulk of the material migrated as a monomer at ~12 kDa (calculated molecular mass, 11.3 kDa). In addition to this band, a number of high molecular mass species, corresponding to polymers of various sizes, were seen (Fig. 1, lane 1). The antibodies used bind with higher avidity to polymers than to monomers, and the amount of polymers is therefore likely to be overrepresented in the immunoblots.3 Proteinase K (10 µg/ml) treatment of polymerized C100 cleaved the protein and generated several fragments with molecular masses lower than that of native C100 (Fig. 1, lane 2). Addition of higher concentrations of proteinase K yielded a 6E10-reacting species with a molecular mass indistinguishable from that of synthetic Abeta (Fig. 1, compare lanes 3 and 4 with 5). The Abeta -specific antibody 4G8, recognizing amino acids 18-21 in Abeta , yielded an essentially identical pattern (data not shown). Analysis with antibody 369 showed that all material corresponding to the cytoplasmic domain of C100 had been degraded by the proteinase K (100 and 1000 µg/ml) treatment (data not shown).


Fig. 1. Generation of Abeta -like peptide from polymerized C100 through nonspecific proteolysis. Identical amounts of polymerized C100 were subjected to digestion with the indicated concentrations of proteinase K (Prot. K) for 5 h at 37 °C before termination of the reactions with PMSF (1 mM) and urea (9 M). Synthetic Abeta 1-40 was loaded on lane 5 as a reference. The positions of the molecular mass standards (kDa) are shown to the left.
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Polymerization of C100 Is Required for Generation of Abeta

In the next series of experiments, it was investigated whether polymerization of C100 is required for the generation of Abeta in a reaction catalyzed by a nonspecific protease. Purified C100 was incubated in TBS at 37 °C for the indicated periods (Fig. 2). To determine whether the polymerization and protease resistance is a reversible reaction, one polymerized aliquot was treated with formic acid, which dissociates the fibrils, and lyophilized prior to digestion. After addition of proteinase K, the samples were incubated for 5 h. The reaction was then stopped, and the samples were dissolved and analyzed by immunoblotting (Fig. 2). The nondigested sample showed a smearlike pattern, indicating that the fibrils had not been completely dissolved (Fig. 2, lane 1). Nonpolymerized C100 was almost completely digested, and no Abeta -like species were detected (Fig. 2, lane 3), indicating that monomeric C100 cannot serve as a substrate for the generation of Abeta by nonspecific proteolysis. This is in agreement with previous results using monomeric Abeta as a substrate (16). Polymerized C100 that had been treated with formic acid to dissociate the fibrils was not totally digested, and a faint band in the Abeta region (~4 kDa) was detected (Fig. 2, lane 2). The difference between this sample and the nonpolymerized sample may be due to the fact that the fibrils formed were not completely dissociated in formic acid. The polymerized and proteinase K-treated samples gave a strong signal in the Abeta region, i.e. ~4 kDa (Fig. 2, lanes 4 and 5).


Fig. 2. Effect of C100 polymerization on the generation of an Abeta -like peptide. Purified C100 was allowed to polymerize at 37 °C for the indicated periods. One sample was not digested (lane 1). One sample was treated with formic acid to dissociate the fibrils prior to digestion (lane 2). This sample, a nonpolymerized sample (lane 3), and polymerized samples (lanes 4 and 5) were treated with proteinase K (100 µg/ml) for 5 h at 37 °C. The positions of the molecular mass standards (kDa) are shown to the left.
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Electron Microscopy of C100 Fibrils and Infected Cells

Purified and polymerized C100, digested with proteinase K or nondigested, were adsorbed to grids, negatively stained with uranyl acetate, and examined by electron microscopy. Like Abeta , C100 formed fibrils with a diameter of about 5-8 nm, typically arranged in tight bundles (Fig. 3, A and B). Immunogold staining, with 6E10 and 369 as primary antibodies, revealed that the fibrils contained epitopes located both in the Abeta and the cytoplasmic domains of C100 (Fig. 3D). Thus, it was concluded that the fibrils consisted of intact C100. Electron microscopic examination of the proteinase K-treated C100 fibrils revealed a fibrillar structure, indistinguishable from that of genuine Abeta (Fig. 3, compare A and C). Moreover, immunogold staining revealed that the proteinase K-treated C100 fibrils remained reactive with 6E10 but not with 369 (data not shown). Hence, it is concluded that nonspecific proteolysis of C100 can generate an Abeta species electrophoretically, immunologically, and morphologically equivalent to purified Abeta .


Fig. 3. Electron microscopic examination of fibrils and infected Sf9 cells. A, fibrils of Abeta purified from amyloid extracted from brain tissue, negatively stained with uranyl acetate. B, polymerized C100, stained as in A. C, polymerized, proteinase K-digested C100, stained as in A. D, immunogold staining of C100 fibrils with the Abeta -specific antibody 6E10 (10 nm gold) and antibody 369, which is specific for the C terminus of C100, (20 nm gold). E, infected Sf9 cells. Sf9 cells infected with recombinant baculovirus containing the C100 DNA-fragment were fixed in 2% formaldehyde/0.1% glutaraldehyde in phosphate-buffered saline, pH 7.3, dehydrated in graded ethanol, and embedded in LR White. Thin sections were immunostained using 369 as the primary antibody and anti-rabbit IgG conjugated to 10 nm colloidal gold particles as the secondary antibody. Labeled membrane vesicles and fragments are seen in a cell in an early lytic phase of virus infection. Bars, 100 nm.
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The intracellular localization of C100 in Sf9 cells infected with recombinant baculovirus containing DNA coding for C100 was examined with immunogold staining of thin sections of fixed and embedded cells. The bulk of the immunoreactivity was confined to a complex system of membranes found in both the cytoplasm and the nucleus (Fig. 3E), indicating that recombinant C100 is indeed inserted in membranes.

Proteolytic Digestion of Polymerized C100 in Cell Membranes

Like polymerized synthetic Abeta (16), the Abeta region of polymerized C100 was apparently partly resistant to proteolytic degradation. An intriguing possibility was therefore that C100 inserted into cell membranes can aggregate and thereby develop protease resistance. It was therefore investigated whether membrane-inserted C100 can polymerize, similar to the purified protein. For this purpose, a membrane preparation from Sf9 cells, infected with baculovirus-containing C100 DNA, was subjected to SDS-PAGE and immunoblot analysis with antibodies 6E10 and 369. Purified, monomeric C100 was loaded on the gel as a reference. The C100 protein from a crude membrane preparation displayed a smearlike pattern, strongly suggesting that C100 had formed polymers of different sizes (Fig. 4, lane 1), whereas the purified protein migrated as a single species with an apparent molecular mass of ~12 kDa (calculated molecular mass, 11.3 kDa) (Fig. 4, lane 2). It is unlikely that the polymers were formed after the addition of sample buffer, which contained a highly denaturing chaotrope (urea) and detergent (SDS), since purified monomeric C100 did not form polymers when it was analyzed under the same conditions. Hence, it is concluded that C100 can polymerize while still inserted in cell membranes.


Fig. 4. C100 can polymerize in cell membranes. A membrane preparation from Sf9 cells infected with recombinant baculovirus containing C100 DNA (lane 1) and C100 purified by size exclusion chromatography (lane 2) were dissolved in Laemmli sample buffer and subjected to SDS-PAGE and immunoblot analysis with antibody 6E10. The positions of the molecular mass standards (kDa) are shown to the left.
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It was then examined whether C100 inserted in the membrane of transfected cells can serve as a substrate for the generation of Abeta , analogous to purified and polymerized C100. A membrane fraction from Sf9 cells infected with recombinant baculovirus containing C100 DNA was incubated overnight in TBS with or without the zwitterionic detergent CHAPS. CHAPS was added to dissolve potential membrane vesicles, thereby making both the N and C termini of C100 accessible to the protease. The CHAPS molecules act by hydrophobic interaction with the transmembrane region of C100, possibly substituting for the phospholipid bilayer, and the transmembrane region is thereby still shielded from proteases. After digestion with proteinase K for 5 h, the samples were analyzed by immunoblotting (Fig. 5). As seen in Fig. 5, lane 3, a fragment with a slightly higher apparent molecular mass than Abeta , ~6 kDa, was formed. The addition of CHAPS did not alter the apparent molecular mass of the proteolytic fragment generated by proteinase K (Fig. 5, lanes 2 and 4). A cleavage at lysine 53 (which is the first amino acid residue C-terminal to the predicted transmembrane region of C100) would generate a 5.9-kDa fragment. The detected species corresponds in molecular mass and immunoreactivity to such a fragment, indicating that the Abeta domain of C100 can form a protease-resistant core while still inserted in membranes, and that the transmembrane region is shielded from proteolysis by phospholipids and CHAPS. The experiment was repeated using trypsin instead of proteinase K. Trypsin cleaves C-terminally to lysine and arginine residues, a putative cleavage site being lysine 53. A cleavage after this residue should generate a 5.9-kDa fragment, provided that putative tryptic cleavage sites within the Abeta domain of C100 (i.e. Arg5, Lys16, and Lys28) were protease resistant. A 6E10 immunoreactive fragment with the same apparent molecular mass as the fragment generated by proteinase K was detected (data not shown). It is therefore concluded that C100 inserted in membranes can polymerize and develop protease resistance, and that dissolution of the phospholipid bilayer is necessary to generate the free C terminus of Abeta .


Fig. 5. Abeta can be generated from C100 inserted in membranes. Membranes from Sf9 cells infected with recombinant baculovirus containing the C100 gene were subjected to treatment with proteinase K (50 µg/ml) for 5 h at 37 °C in the presence or absence of CHAPS (1%) and thereafter analyzed by SDS-PAGE and immunoblotting with antibody 6E10. The positions of the molecular mass standards (kDa) are shown to the left.
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The Cytoplasmic Domain of C100 Forms Non-Protease-resistant Fibrils

To investigate the role of the cytoplasmic domain of C100 in fibril formation, a synthetic peptide corresponding to this part of C100 was incubated under the same conditions as purified C100. When the incubated peptide was analyzed by electron microscopy, fibrils similar to those generated from Abeta and C100 were found (data not shown). Thus, the cytoplasmic domain of C100 may be involved in the polymerization of C100, since this region can form fibrils per se. After digestion with proteinase K, no immunoreactivity was found when the sample was analyzed by SDS-PAGE followed by immunoblotting with 369 as a primary antibody. Apparently, the fibrils formed from the cytoplasmic domain of C100 were, in agreement with our other experiments, not protease-resistant.

CHO Cells Stably Transfected with beta -APP751SW Generate SDS-insoluble C100 Fragments

To investigate whether C100 generated from full-length beta -APP exists in an aggregated form, CHO cells were stably transfected with beta -APP751SW (18). The Swedish mutation was chosen, since it produces approximately five times more Abeta than the wild-type gene and, according to the present hypothesis, therefore should produce more C100 polymers. A postnuclear supernatant from CHO cells, stably transfected with beta -APP751SW, was centrifuged at 125,000 × g for 1 h. The resulting pellet was extracted with 1% SDS in TBS and centrifuged once more. The supernatant was lyophilized, and the pellet was dissolved in 9 M urea at pH 10 for 24 h. After addition of urea to the SDS-extracted sample and SDS to the urea-extracted sample, Laemmli sample buffer was added, and the samples were subjected to SDS-PAGE. Immunoblot analysis, using 6E10 or 369 as primary antibody, showed a remarkable difference between the urea-extracted sample and the SDS extract (Fig. 6). No immunoreactivity could be detected in the SDS extract, either with 6E10 or with 369 as the primary antibody. The urea treated sample displayed, with 6E10 as the primary antibody, one intense band in the C100 region. The same band was detected with 369, suggesting that the detected species corresponds to C100. A fragment with slightly lower molecular mass was also detected with antibody 369, probably corresponding to the C-terminal fragment of beta -APP generated by alpha -secretase cleavage, the Esch fragment (7). The absence of immunoreactive fragments in the SDS extract indicates that C100 generated from beta -APP751SW is tightly bound and, in accordance with our earlier results, probably occurs as a polymer.


Fig. 6. Extraction of C100-like material from mammalian cells. Left panel, CHO cell membranes extracted with urea or SDS as described under "Experimental Procedures" and purified C100 were separated by SDS-PAGE. Following electroblotting, immunoreactive material was visualized using the Abeta region-specific antibody 6E10. Right panel, the same samples as in A, but here immunoreactivity was visualized using antibody 369, which is specific for the cytoplasmic region of beta -APP. Filled arrowhead, C100; open arrowhead, the Esch fragment.
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DISCUSSION

Here, we have presented evidence that Abeta can be generated from polymerized, but not from nonpolymerized, C100 by the nonspecific proteolytic activity of proteinase K.

C100, a metabolic fragment generated through beta -secretase cleavage of beta -APP (13, 20-23), can polymerize and form fibrils similar to those of Abeta (24). In our experiments, polymerization occurred both when the protein was inserted in cell membranes and after purification of the protein. A reasonable explanation is that C100 polymerizes through interaction between Abeta domains, since Abeta readily forms fibrils (25). The cytoplasmic domain of C100 also forms fibrils per se, and this interaction may enhance the ability of C100 to form fibrils.

Polymerization of synthetic Abeta is associated with increased resistance of the molecule to proteolytic enzymes with varying substrate specificities in vitro (16). It has also been shown that aggregates of synthetic Abeta 1-42 internalized by cultured human skin fibroblasts are stable for several days and co-localizes with late endosomes and lysosomes (26). Therefore, we wanted to investigate whether the Abeta domains in C100 could form "protease-resistant cores" through a similar mechanism. Intracellular proteolysis in endosomes and lysosomes involves several endoproteases and exoproteases with various substrate specificities (15, 27). Since it would be difficult to mimic this combination of enzymes in a reconstituted system, we decided to use proteinase K, a bacterial protease with a wide substrate specificity, cleaving all natural peptide bonds (19). This enzyme has previously been suggested to be capable of generating Abeta -like peptides from beta -APP (28). Digestion of polymerized C100 with proteinase K rendered a fragment chromatographically, immunologically, and morphologically indistinguishable from that of synthetic Abeta . These results demonstrate that: (i) polymerization of C100 is associated with formation of a protease-resistant core of tightly bound Abeta domains; and (ii) an enzyme cleaving all natural peptide bonds can substitute for the putative gamma -secretase. When C100 was digested in cell membranes, in the presence or absence of detergent, a species with slightly higher molecular mass than Abeta was formed. The transmembrane domain of C100 is apparently shielded from proteolysis by phospholipids or detergent. However, the phospholipid membrane can be degraded in lysosomes, exposing the transmembrane domain of the protein to proteases. The proposed model is described schematically in Fig. 7.


Fig. 7. Proposed model of generation of the Abeta peptide in cells. When beta -APP is cleaved by beta -secretase, en route to or at the plasma membrane, C100 is generated (1). A fraction of these molecules bind to each other by interactions between their Abeta domains and possibly also between their cytoplasmic domains. Thereby, protease-resistant Abeta cores are formed (2). Bound and free C100 molecules are transported to an organelle, in which they are exposed to a multitude of proteolytic enzymes (3). Nonresistant parts of the molecules are degraded (4), whereas the protease-resistant Abeta cores remain intact and subsequently are secreted from the cells (5).
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It was also demonstrated that most of the C100 produced by beta -APP751SW-transfected CHO cells was SDS-insoluble. The C100 produced by these cells could, like C100 polymers formed from purified C100 or C100 in Sf9 cells, be dissolved in 9 M urea at pH 10. The SDS insolubility is also characteristic for the Abeta fibrils found in Alzheimer's disease brains (29). These findings indicate that the C100 molecules produced by the beta -APP751SW-transfected cells are tightly associated, probably in polymeric form. If present in human brain cells, such polymers could be partially degraded, leaving a protease-resistant core of polymeric or oligomeric Abeta .

An important and obvious question is whether Abeta can be formed through nonspecific proteolysis not only in the artificial systems described here but also in intact cells. A recent study (30) demonstrated that Abeta secreted from cells was present as soluble oligomers of various size. The oligomers were detectable after separation under denaturing conditions in SDS-PAGE, indicating that they were joined by strong bonds. It can be speculated that the oligomers had been formed prior to gamma -secretase cleavage and secretion in a reaction similar to the one described here, rather than from monomeric Abeta in the cell media. When beta -APP-transfected human kidney cells are treated with aggregated Abeta 1-42, the accumulation of stable, insoluble amyloidogenic aggregates is stimulated (31). One possible explanation to this finding is that the Abeta domains in endogenously produced C100 bind to internalized Abeta 1-42 and thereby form protease-resistant cores. The fact that an intact fragment corresponding to C100 lacking the Abeta domain has yet not been detected in cells may also be an argument against the idea that the gamma -secretase is a specific enzyme (see Ref. 21).

Apparently, nonspecific protease activity cleaving any peptide bond can serve as gamma -secretase, if the substrate has the right higher order structure. This model suggests that Abeta can be formed in organelles such as endosomes and lysosomes, containing a multitude of proteases covering a broad spectrum of substrate specificities (15, 27). Low pH promotes formation of Abeta fibrils in vitro (32). Through a similar mechanism, the acidic environment in late endosomes and lysosomes may enhance the formation of C100 fibrils with a protease-resistant core corresponding to Abeta .

The mechanism for Abeta formation proposed here may have pharmacological implications. It has been suggested that protease inhibitors capable of inhibiting beta - and gamma -secretase activity may be used to reduce Abeta production in vivo and, therefore, are candidate drugs for the treatment of Alzheimer's disease-associated amyloidosis (3). Considering the present model, it would not be possible to find a selective gamma -secretase inhibitor. Alternatively, a molecule capable of antagonizing C100 polymerization would prevent the generation of a protease-resistant core of Abeta , thereby preventing amyloidogenesis in Alzheimer's disease.


FOOTNOTES

*   This study was supported in part by grants from the Swedish Medical Research Council (to J. T. and L. T.) and the Swedish Heart Lung Foundation (to J. T.), USPHS Grant AG11508 (to S. E. G.), and the King Gustaf V 80th Birthday Fund (to J. T.). The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Dagger Dagger    Recipient of fellowships from the Axel Margret Ax:son Johnson Foundation, the Jeansson Foundation, the Sandoz Foundation, and the Nicholson Foundation.
   Supported in part by a fellowship from The Swedish Society for Medical Research. Present address: Laboratory of Molecular and Cellular Neuroscience The Rockefeller University 1230 York Ave., New York, NY 10021.
§   To whom correspondence should be addressed. Tel.: 46-8-729-46-02; Fax: 46-8-34-19-39; E-mail: tjern{at}ian.ks.se.
1    The abbreviations used are: Abeta , Alzheimer amyloid beta -peptide; beta -APP, Alzheimer beta -amyloid precursor protein; C100, C-terminal 100 amino acids of beta -APP; TBS, Tris-buffered saline; PMSF, phenylmethylsulfonyl fluoride; PAGE, polyacrylamide gel electrophoresis; CHO, Chinese hamster ovary; CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid.
2    F. Lindqvist, J. Georgieva, L. O. Tjernberg, C. Lilliehöök, S. E. Gandy, L. Terenius, and C. Nordstedt, manuscript in preparation.
3    L. O. Tjernberg J. Näslund, and C. Nordstedt, unpublished observations.

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