Transcription of the Acanthamoeba TATA-binding Protein Gene
A SINGLE TRANSCRIPTION FACTOR ACTS BOTH AS AN ACTIVATOR AND A REPRESSOR*

(Received for publication, September 12, 1996, and in revised form, November 12, 1996)

Weibiao Huang and Erik Bateman Dagger

From the Department of Microbiology and Molecular Genetics, Cell and Molecular Biology Program, Markey Center for Molecular Genetics, University of Vermont, Burlington, Vermont 05405

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
Acknowledgment
REFERENCES


ABSTRACT

Transcription of the Acanthamoeba TATA-binding protein (TBP) gene is regulated by TBP promoter-binding factor (TPBF), a previously described transactivator that binds as a tetramer to the TBP Promoter Element (TPE) and stimulates transcription up to 10-fold in vitro. Here we report that TPBF also functions as a transcription repressor by binding to a negative cis-element, located between the TATA box and the transcription initiation site. The negative element, referred to as the nTPE, is structurally similar to the TPE, and its disruption increases the transcription potency of the TBP promoter. TPBF binds to the nTPE, as demonstrated by mobility shift assays. However, the binding affinity of TPBF for the nTPE is about 10-fold lower than for the TPE. When placed upstream of the TATA box, the nTPE has very little effect on transcription. However, it inhibits transcription when placed at several positions downstream of the TATA box. Mechanistic studies with the TBP promoter suggest that binding of TPBF to the nTPE not only prevents TBP from binding to the TATA box but also displaces bound TBP, thereby inhibiting further assembly of the preinitiation complex. These results suggest a mechanism in which the cellular TPBF concentration controls the level of TBP gene transcription and show that a single factor can be stimulatory, inhibitory, or neutral depending on the sequence and the context of its binding site.


INTRODUCTION

Modulation of gene expression at the level of transcription initiation is a major regulatory strategy for eukaryotic cells to control their responses to intra- or extracellular stimuli. Similarly, the level of production of housekeeping genes is also tightly regulated at the level of promoter efficiency. Transcription initiation on eukaryotic promoters involves the sequential addition of individual transcription factors through protein-DNA and/or protein-protein interactions (1). While accurate initiation of transcription from most eukaryotic class II promoters requires RNA polymerase II as well as a set of general transcription factors that includes TFIID,1 TFIIB, TFIIF, TFIIE, and TFIIH (2), the level of transcription is mainly regulated by DNA-binding, sequence-specific transcription factors, also known as activators or repressors. Sequence-specific transcription factors bind to promoter elements via DNA-binding motifs and modulate transcription positively or negatively through direct or indirect (via coactivators) communication with the general transcription machinery (3). Most promoter elements are recognized by one single transcription factor. However, there are several examples in which one promoter element can be recognized by multiple factors or seemingly unrelated DNA elements can be recognized by a single factor (4-9).

TATA-Binding Protein (TBP) is a highly conserved eukaryotic basal transcription factor that is required for transcription by all three RNA polymerases both in vitro and in vivo (10-12). TBP can associate with distinct sets of proteins (TBP-associated factors) thereby forming the complexes TIF (13), TFIID (14), and TFIIIB (15, 16) required for RNA polymerase I, II, and III transcription, respectively.

Due to the central role that TBP plays in eukaryotic transcription, changes to cellular TBP levels would impact all biological events occurring during cell growth and differentiation. It is thus important to understand the regulatory mechanisms that control TBP gene transcription. Although the genomic DNAs encoding TBP have been cloned from various organisms (17-20), regulation of TBP gene expression is far from fully understood. We have previously performed detailed promoter mapping studies to investigate how TBP gene transcription is regulated in Acanthamoeba. Two major cis-elements that are necessary for efficient transcription were identified. One is the TATA box, which is required for basal transcription. The other major control element is the TBP promoter element (TPE), a 23-base pair element located between positions -94 and -72 of the TBP gene promoter, which can stimulate transcription up to 10-fold in vitro (21). A regulatory protein called TPBF, which specifically binds to the TPE, was previously purified from Acanthamoeba (21, 22), and the cDNA encoding TPBF was subsequently isolated (23).

TPBF is a novel tetrameric DNA-binding protein. It contains a C-terminal coiled-coil domain, which drives tetramerization. The pattern of protein-DNA contacts between tetrameric TPBF and TPE, which resembles the proposed model for the interaction between the p53 tetramer and its target DNA (24), is distinct from that produced by other coiled-coil transcription factors (22). Our domain mapping studies also suggest that TPBF, like p53, has an apparently large central region involved in specific DNA binding. TPBF is likely to bind to other Acanthamoeba promoter elements such as that of the polyubiquitin gene, which contains a near perfect TPE (25).

Several previous observations suggested, but did not prove, that TBP gene expression is subject to negative control by a TPBF-binding element between positions -5 and -19 of the TBP gene promoter. First, in vitro transcription of the TBP gene is surprisingly efficient given the low abundance of TBP mRNA in vivo (17). Second, analyses of 3' deletions showed that removal of sequences between positions -5 and -19 of the TBP gene promoter results in a 10-fold increase in transcription efficiency (26).2 Third, DNase I footprinting showed that at high concentrations TPBF can bind to several sites within the TBP gene promoter including the region between the TATA box and the start site (22). Finally, the addition of recombinant TPBF to extracts partly depleted of TPBF failed to show the expected stimulation of transcription in vitro.

Here we report the identification and characterization of another major cis-element, the nTPE, which is located between the TATA box and the transcription initiation site. The nTPE exerts a strong negative effect on TBP gene transcription in vitro. The nTPE is structurally similar to the TPE and is also specifically bound by TPBF. Comparison of TPBF binding affinities suggests that TPBF can sequentially bind to these two elements and thereby modulate TBP gene expression. By adding TPBF back into TPBF-free nuclear extracts, we show that the TPBF concentration determines the level of TBP gene transcription. Finally, we suggest a mechanism of TPBF-induced repression by showing that TPBF, when bound to the nTPE, actively displaces TBP from the TATA box. A working model of how TPBF regulates TBP gene expression is presented.


EXPERIMENTAL PROCEDURES

Oligonucleotides

The oligonucleotides used in this research were as follows: TPEu, 5'-AACAAGCTGAGAAAAAACCAGGATCGG-3'; TPEb, 5'-CCGATCCTGGTTTTTTCTCAGCTTGTT-3'; mTPEu, 5'-AACCAGCTGAGAAACAACCAGGATAGG-3'; mTPEb, 5'-CCTATCCTGGTTGTTTCTCAGCTGGTT-3'; nTPEu, 5'-AAGGGGCCAATTTTTTTGTTGATTTGTTG-3'; nTPEb, 5'-CAACAAATCAACAAAAAAATTGGCCCCTT-3'; nTPEm, 5'-CAACAAATCAACGCTCGCGTTGGCCCCTT-3'; RTA3, 5'-CAATTTCACACAGGAAACAGCTATGAC-3'; RTA4, 5'-GCGATTAAGTTGGGTAACGCCAGGGTTT-3'; TBP, 5'-CGCCATGCCCGCGCTCTGAAGGACATTCGT-3'; Rev, 5'-AACAGCTATGACCATG-3', T7 5'-AATACGACTCACTATAG-3'; KS, 5'-CGAGGTCGACGGTATCG-3'.

Construction of Plasmids as the Templates for in Vitro Transcription

The DNA fragment for making the nTPE linker-scanning mutant was generated by PCR amplification of a wild type TBP promoter using primers TPEu and nTPEm. A linker-scanning mutant was then constructed by inserting the fragment into the EcoRV site of the pSK(-) vector. Similarly, a wild type control construct was generated by using primers TPEu and nTPEb. Plasmid TATA1 was made by inserting a synthetic TATA box (-TATATAAG-) into the EcoRI and BamHI sites of the pSK(-) vector. Plasmids TATA1-TPEa, TATA1-TPEb, and TATA1-TPEc were derived from plasmid TATA1 by insertion of double-stranded TPE DNA into the HincII, EcoRV, and KpnI sites, respectively. The same approach was used to generate plasmids TATA1-nTPEa, TATA1-nTPEb, and TATA1-nTPEc.

Affinity Purification of Anti-TPBF Serum

1 mg of purified, recombinant TPBF was fractionated on an SDS-polyacrylamide gel and transferred electrophoretically to a nitrocellulose membrane. The membrane was quickly stained with 0.2% Ponceau S in 1% acetic acid. The TPBF band was cut out, and the remaining stain was removed by washing with phosphate-buffered saline. The 1 × 5-cm strip containing TPBF was blocked in 20 ml of 5% nonfat dry milk in phosphate-buffered saline for 30 min with gentle agitation and then washed three times, each for 5 min, using 20 ml of phosphate-buffered saline. The strip was placed in a 15-ml centrifuge tube and incubated with 3 ml of crude rabbit anti-TPBF serum at 4 °C overnight (23). After the serum was removed, the strip was washed three times using 20 ml of phosphate-buffered saline. Antibodies were eluted by incubation with 1 ml of 0.1 M glycine (pH 2.5), 100 mM NaCl at 4 °C for 30 min. Purified antibodies were quickly neutralized to pH 7.0 by adding 6 M NaOH.

Immunodepletion of TPBF from Acanthamoeba Nuclear Extract

Affinity-purified TPBF antibodies were coupled to CNBr-activated Sepharose 4B (Pharmacia) under the conditions suggested by the manufacturer. Immunodepletion was carried out in a minicolumn containing 1 ml of antibody-coupled Sepharose 4B resin at 4 °C. The column was equilibrated with CB100 (20 mM HEPES (pH 7.5), 0.2 mM EDTA, 10% glycerol, 0.2 mM phenylmethylsulfonyl fluoride, 1 mM benzamidine, 0.1 mM N-tosyl-L-phenylalanine chloromethyl ketone, 2 µg/ml leupeptin, 2 µg/ml pepstatin, 1 mM DTT, and 100 mM KCl). 1 ml of Acanthamoeba nuclear extract (~5 mg in CB100) was loaded, and the flow-through was collected and subjected to another cycle of affinity chromatography. TPBF was quantitatively removed as confirmed by immunoblotting (23).

In Vitro Transcription and Primer Extension

Transcription and primer extension assays were performed essentially as described (21, 23). Briefly, 200 ng of supercoiled plasmid templates were incubated with 50 µg of Acanthamoeba nuclear extracts in 50-µl reactions containing 25 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM DTT, 75 mM potassium acetate, 0.02 mM EDTA, 2% glycerol, 1 unit of RNasin (Life Technologies, Inc.), and 0.4 mM each NTP. Reactions were carried out at 30 °C for 1 h. Transcripts were extracted with phenol-chloroform twice, precipitated with ethanol, and dissolved in 10 µl of annealing buffer containing 20 mM Tris-HCl (pH 8.3), 400 mM KCl, and 50,000-100,000 cpm of 32P-labeled primer. Annealing was performed by slowly cooling from 65 °C to room temperature. Primer extensions were started by adding 4 µl of 10 × RT buffer (500 mM Tris-HCl (pH 8.3), 60 mM MgCl2, 25 mM DTT), 4 µl of dNTPs (2.5 mM each), 1 unit of RNasin, and 20 units of Superscript II into a total volume of 40 µl, followed by incubation for 1 h at 45 °C. Primer extension products were precipitated and analyzed by electrophoresis in a 6% polyacrylamide, M urea gel in TBE buffer (27).

Purification of Recombinant TPBF and TBP

Purifications of recombinant TPBF and TBP proteins were performed essentially as described elsewhere (23, 28). Briefly, the pET3a vector containing the TPBF or TBP cDNA was freshly transformed into Escherichia coli strain LE392. The E. coli cells were grown to an A600 of 0.8 and infected with lambda CE6 (Novagen). Cells were harvested after 3.5 h of infection, and the cells were lysed by sonication. For TPBF purification, the cell lysate was subjected to nickel affinity chromatography. TPBF was eluted with 300 mM imidazole (pH 7.5). Fractions containing TPBF were pooled and concentrated using a Centricon 10 spin column (Amicon). Purified proteins were stored at -70 °C in buffer containing 20 mM HEPES (pH 7.5), 10% glycerol, 0.1 mM EDTA, and 0.2 mM phenylmethylsulfonyl fluoride.

For the production of recombinant Acanthamoeba TBP, the cell lysate was applied to DEAE-cellulose (Whatman) equilibrated with column buffer (20 mM HEPES (pH 7.5), 10% glycerol, 0.1 mM EDTA, 1 mM DTT, 1 mM phenylmethylsulfonyl fluoride, 0.1 mM benzamidine, 0.1% Triton X-100) containing 200 mM KCl. The flow-through was collected and applied to an Affi-gel heparin column (Bio-Rad) equilibrated with column buffer containing 200 mM KCl. TBP was eluted with column buffer containing 500 mM KCl. Active fractions, as judged by mobility shift assay, were pooled together and dialyzed against column buffer containing 100 mM KCl. TBP was purified to ~80% homogeneity.

Gel Mobility Shift Assays and Probe Preparations

Approximately 0.5 ng of 32P-labeled probe was mixed with the indicated amount of protein in each reaction. The binding reactions were carried out in a total volume of 15 µl containing 20 mM HEPES (pH 7.5), 50 mM KCl, 7.5 mM MgCl2, 0.2 mM EDTA, 10% glycerol, and 0.5 mM DTT at 30 °C for 20 min. In the experiments shown in Fig. 6, HindIII treatment was done by adding 10 units of enzyme and incubating for an additional 10 min. Probes containing single TPE or nTPE elements were produced by annealing appropriate complementary oligonucleotides and end-labeling using T4 polynucleotide kinase. Full-length TBP promoter was made by PCR amplification from the plasmid template TBP-97 (21) using primers TPEu and nTPEb. TPE-nTPE probe containing a HindIII site was made by inserting TPE and nTPE elements into the EcoRV and HincII sites of the pSK(-) vector, respectively, and then amplified using primers TPEu and nTPEu. All PCR products were gel-purified and labeled with 32P. Probe TPE-nTPE labeled with 32P at either the TPE side or the nTPE side was generated by PCR using an appropriate 32P-labeled primer and its corresponding pair. Probe TATA-nTPE was generated by PCR amplification of TBP-35 template (21) using primers KS and nTPEb.


Fig. 6.

TPBF is the only protein responsible for activation and repression. A, basal transcription remains unchanged after TPBF depletion. Immunodepletion, Western blotting, and in vitro transcription assays were performed as described under "Experimental Procedures." On the left and in the middle are Western blots assaying TPBF and TBP levels, respectively, in nuclear extract before and after immunodepletion. On the right is the transcription assay of the TATA1 promoter (diagramed in Fig. 5), using nuclear extract before and after immunodepletion. The indicated amounts of TPBF were included to assess the effect of TPBF on basal transcription. B, transcription recovery from TPBF-depleted nuclear extract. The templates assayed and the primers being used to detect the transcripts are indicated at the top and bottom, respectively. Template TBP-97 is the 5' deletion of the TBP promoter up to nucleotide -97. Template TATA1-TPEa is diagrammed in Fig. 5. RTA3 detects downstream transcription, while RTA4 detects transcription toward the TPE. Whether nuclear extract was TPBF-depleted or not and how much TPBF was added are indicated above each lane. Specific products are shown by triangles. C, TPBF is responsible for nTPE-induced repression in a native TBP promoter, TBP-35. Lanes 1 and 2 show transcription activities from a control nuclear extract and a TPBF-depleted nuclear extract, respectively. Lanes 3-7 show transcription activities after the indicated amounts of recombinant TPBF were added back into the TPBF-depleted nuclear extract. Transcripts were detected by primer extension with primer TBP.


[View Larger Version of this Image (18K GIF file)]



RESULTS

The TBP Gene Promoter Contains a Negative cis-Element between the TATA Box and the Transcription Initiation Site

Inspection of the DNA sequence between the TATA box and the transcription initiation site of the TBP gene promoter identifies a sequence that is similar to the upstream TPBF recognition site, the TPE, but inverted (Fig. 1A). In order to consolidate earlier results (see the Introduction) and to establish that this sequence functions as a negative element in the context of the native TBP gene promoter, we made a linker-scanning mutant in which the run of T nucleotides between nucleotides -14 and -8 of the TBP promoter are replaced with 5'-CGCGAGC-3' (Fig. 1B, top). The promoter activity of the linker-scanning mutant was tested by in vitro transcription and was found to be 4-fold higher than that of a wild type promoter (Fig. 1B, bottom). This result clearly demonstrates that the sequence is a negative element in the context of the TBP promoter. The 4-fold effect of the linker-scanning mutagenesis is less than the 10-fold effect found with a simple deletion to -19 (26). This could be because the TPE-related sequence is not completely disrupted by linker scanning. However, as shown below, the nTPE is both necessary and sufficient for negative regulation of the TBP gene.


Fig. 1. A TPE-like negative sequence exists between the TATA box and transcription start site of the TBP promoter. A, nucleotide sequence of the TBP promoter region from -100 to +11. Two TPE-related sequences are underlined. The TATA box and the transcription start site are indicated by the dark bar and the arrow, respectively. B, disruption of the run of seven T nucleotides within the TPE-like sequence. A linker-scanning mutant was constructed as described under "Experimental Procedures." Lane 1 is transcription from a wild type TBP promoter, and lane 2 is from the linker-scanning mutant. Primer Rev was used for analyzing transcripts.
[View Larger Version of this Image (18K GIF file)]


TPBF Binds to the Negative Element in the TBP Promoter

Alignment of the TPE, as defined by chemical interference (22) and the inhibitory sequence between the TATA box and the transcription start site, shows that 13 out of 23 base pairs are identical between these two sequences (Fig. 2A). The inhibitory sequence contains half of the bases that form contacts with TPBF as suggested by chemical interference assays on the TPE sequence (22). Since this sequence can inhibit transcription, we refer to it as the nTPE.


Fig. 2. Specific interaction between TPBF and the nTPE. A, sequence alignment of the TPE and the nTPE. B, probes used in the mobility shift assays are indicated above each lane. 50 ng of either full-length TPBF (lanes 1, 3, and 5-7) or mutant TPBF (lanes 2 and 4) were included in each reaction. The specificity of TPBF binding to the nTPE was tested by using either affinity-purified anti-TPBF (lane 5), a specific competitor (lane 6), or a mutant competitor (lane 7). Complexes are identified on the right.
[View Larger Version of this Image (41K GIF file)]


The possibility that the nTPE is specifically recognized by TPBF was suggested by its striking sequence similarity to the TPE. In order to directly demonstrate that TPBF can bind to the nTPE, a pair of complementary oligonucleotides corresponding to nucleotides -24 to +2 of the TBP promoter were synthesized. Mobility shift assays using purified recombinant TPBF clearly show an interaction between the nTPE and TPBF (Fig. 2B, lane 3). The interaction was specific, as suggested by both antibody supershift and competition experiments in which the TPE, but not a mutant TPE, prevent binding to the nTPE (Fig. 2B). A TPBF deletion mutant, in which the tetramerization region is removed, totally abolished the interaction, indicating that TPBF tetramerization is also necessary for nTPE binding. Moreover, the complex between TPBF and the nTPE has the same mobility as the complex between TPBF and the TPE. We infer that TPBF binds to the nTPE as a tetramer as well.

The Inhibitory nTPE Is a Much Weaker TPBF Binding Element than the Activating TPE

In order to further characterize the nTPE and understand its function in TBP gene expression, we compared the affinities of TPBF binding to the TPE and to the nTPE. A TPBF titration experiment was done with identical amounts of either the TPE or the nTPE in mobility shift assays (Fig. 3). Quantitative analyses of the intensity of the shifted bands indicated that 10-fold more TPBF was needed for the nTPE probe to achieve the same level of occupancy as the TPE probe. The affinity between TPBF and the TPE is therefore approximately 10-fold higher than that between TPBF and the nTPE.


Fig. 3. Different binding affinities of TPBF for the TPE and the nTPE. Equal amounts of each probe were incubated with the indicated amount of TPBF under the conditions described under "Experimental Procedures." The complexes shown in lanes 3, 4, 13, and 14 were quantified by phosphor imaging. The relative values were 2.25, 5.38, 1.93, and 6.06, respectively.
[View Larger Version of this Image (54K GIF file)]


The difference in binding affinities between TPBF and these two binding sites provides a hypothetical basis for regulation of TBP gene expression by TPBF. At low concentration, TPBF predominantly occupies the high affinity site that leads to transcription activation. At higher concentrations, it occupies both binding sites and inhibits transcription. To directly test this hypothesis, we synthesized a probe containing both the TPE and the nTPE with a HindIII site between them (Fig. 4, top). When the probe was tested for TPBF binding using a mobility shift assay, we observed one single complex at low TPBF concentration (Fig. 4). With increasing amounts of TPBF, a second higher molecular weight complex appeared. A smooth transition from the lower molecular weight complex to the higher molecular weight complex was observed as the TPBF concentration increased. When the TPBF amount reached 100 ng, only the higher molecular weight complex, corresponding to occupancy of both sites, was present.


Fig. 4. TPBF sequentially binds to a fragment containing both the TPE and the nTPE. The probe containing both the TPE and the nTPE is diagrammed at the top. The asterisk indicates the location of 32P label. The contents of each binding reaction are shown above each lane. Lanes 1 and 8 were treated with HindIII as described under "Experimental Procedures." Complexes corresponding to the occupancy of a single site or both sites are identified as single or double.
[View Larger Version of this Image (87K GIF file)]


In order to determine the order of TPBF binding to the TPE and nTPE sites, we labeled the probe at either the TPE end or the nTPE end and incubated it with TPBF at a concentration that only generated the lower molecular weight complex (Fig. 4, lanes 2 and 9). When the binding reactions were treated with HindIII, the band between TPBF and the nTPE end-labeled probe completely disappeared (Fig. 4, lane 8), suggesting that the band was not due to binding to the nTPE. This was confirmed by the result that the band between TPBF and the TPE end-labeled probe was retained after HindIII digestion (Fig. 4, lane 1). A minor molecular weight change occurred because of trimming of the probe (Fig. 4, compare lanes 1 and 2). These data demonstrate that at low concentration TPBF only binds to the TPE site and that binding to both sites occurs only when the TPBF concentration reaches a certain threshold. We have failed to observe any evidence for cooperative binding by TPBF to the TPE and nTPE in any context (data not shown).

The nTPE Inhibits Transcription When Placed Further Downstream of the TATA Box

To further investigate how the nTPE inhibits transcription and to demonstrate that the nTPE is sufficient for repression, we analyzed the effect of the nTPE on transcription when it is positioned at various sites within a heterologous promoter. The promoter that we used contains a synthetic TATA box, which is able to direct RNA polymerase II transcription in both orientations. In previous studies we showed that the positive element, the TPE, is able to stimulate downstream transcription and inhibit transcription toward it in this promoter context (29). Here, we tested whether the nTPE has similar effects on bidirectional transcription as those of the TPE. We subcloned the nTPE DNA fragment into three different sites (Fig. 5, top), which are 15, 36, and 55 base pairs downstream of the TATA box, respectively, and analyzed the effect of the nTPE on transcription. We employed a primer derived from the pSK(-) vector sequence and assayed the transcription activity of the constructs by primer extension. As shown in Fig. 5, the nTPE was able to repress transcription toward it in any of these three positions as efficiently as the TPE (Fig. 5, compare lanes 12-14 with lanes 9-11). However, unlike the TPE, which is able to stimulate downstream transcription severalfold (Fig. 5, compare lane 1 with lanes 2-4), the nTPE was not able to either activate or repress downstream transcription in any of these positions (Fig. 5, compare lane 1 with lanes 5-7). These data demonstrate that 1) the nTPE alone is a potent inhibitory element; 2) TPBF-induced repression occurs only when its binding site is located downstream of the TATA box, while activation requires the binding site located upstream of the TATA box; and 3) TPBF-induced activation depends on high affinity binding, whereas repression does not. Thus, binding of TPBF to its proximal target sequences is necessary but not sufficient to activate transcription. This was also suggested by the fact that another sequence located between the TPE and the TATA box, although bound by TPBF, does not exert any evident effect on transcription (21). The role of this nonfunctional TPBF binding element in the TBP promoter is unclear.


Fig. 5. Effects of the TPE and the nTPE on transcription activation and repression. Several TATA-containing heterologous promoters including a TPE or an nTPE were used in transcription assays. Each is diagrammed and named above the relevant lane. The orientation of transcription being detected is indicated by the arrow on each construct. Downstream and upstream transcription are indicated by an upward or downward pointing arrow, and detected by primers RTA3 and RTA4, respectively. Downstream transcripts are identified on the left, and upstream transcripts are identified on the right.
[View Larger Version of this Image (58K GIF file)]


TPBF Is Necessary and Sufficient for Activation and Repression

Although TPBF can bind to both the TPE and the nTPE, it was possible that other proteins were also responsible for their effects on transcription. To directly examine the effect of TPBF on activation and repression, we established a TPBF-free Acanthamoeba nuclear extract. As shown in Fig. 6A, we depleted TPBF from a nuclear extract using an affinity-purified anti-TPBF column. Immunodepletion completely removed TPBF (Fig. 6A, left) while not affecting the level of TBP or basal transcription (Fig. 6A, middle and right). We then compared levels of activated transcription supported by the TPBF-depleted nuclear extract with levels supported by normal nuclear extract from promoters containing a TPE. For both the natural TBP promoter and the TATA1-TPEa promoter, depletion of TPBF from the nuclear extract completely abolished the elevated transcription level that was observed with the control nuclear extract (Fig. 6B, lanes 1 and 2 and lanes 5 and 6). The direct involvement of TPBF in transcription repression was also investigated by assaying transcription toward the TPE in the TATA1-TPEa promoter (Fig. 6B, lanes 9-12). We detected at least a 5-fold transcription recovery after TPBF was removed from nuclear extract (Fig. 6B, compare lanes 9 and 10).

To further establish that TPBF is directly responsible for activation and repression, we added purified recombinant TPBF to the TPBF-depleted nuclear extract. We were able to successfully recover both activation and repression with the TATA1-TPEa promoter by adding 100 or 200 ng of recombinant TPBF (Fig. 6B, lanes 7, 8, 11, and 12). However, we were unable to recover activation from a wild type TBP promoter by adding the same amounts of recombinant TPBF (Fig. 6B, lanes 3 and 4). These contradictory results prompted us to analyze the role of TPBF in a minimal TBP promoter (TBP-35) that retains the nTPE (Fig. 6C). Transcription of TBP-35 increased when TPBF was removed from nuclear extracts by immunodepletion (Fig. 6C, lanes 1 and 2). The increase was eliminated by adding back 50 ng of recombinant TPBF (Fig. 6C, lanes 3-5). The activity of TBP-35 was further repressed by adding 100 or 200 ng of TPBF (Fig. 6C, lanes 6 and 7). Overall, the above data demonstrate the presence of both positive and negative TPBF response elements in the TBP gene promoter and show that adding back TPBF has different effects on activation and repression, depending on the context of its binding site.

TBP Gene Expression Is Regulated by a Combination of TPBF-induced Activation and Repression

Given these results, it seemed likely that the expression level of the TBP gene is determined by the sum of TPBF-induced activation and repression. To test this possibility, we performed a TPBF titration experiment on the TBP promoter with TPBF-depleted nuclear extract. The addition of up to 75 ng of recombinant TPBF gave a significant level (3-fold) of transcription recovery from the TPBF-depleted nuclear extract (Fig. 7). However, maximum recovery was achieved with only 10 ng of recombinant TPBF. Greater amounts of TPBF started to inhibit transcription, which eventually dropped to levels lower than basal transcription (Fig. 7). TPBF is thus able to inhibit both activated transcription and basal transcription. We infer that TPBF modulates TBP gene transcription in a concentration-dependent manner.


Fig. 7. TPBF regulates TBP gene transcription in a concentration-dependent manner. A, titration of TPBF into TPBF-depleted nuclear extract. The indicated amounts of TPBF were added into TPBF-depleted nuclear extract to determine its ability to recover activated transcription from the TBP-97 promoter. Untreated nuclear extract was used as a control in lane 1. B, plot of the transcription data quantified by phosphor imaging.
[View Larger Version of this Image (17K GIF file)]


TPBF Prevents Binding and Can Displace TBP from the TBP Promoter

Because the nTPE element is located between the TATA box and the transcription initiation site, an attractive mechanism of repression by TPBF would involve blocking the assembly of the basal transcription machinery. In order to determine whether TBP and TPBF could simultaneously occupy the TATA box and the nTPE, respectively, a TBP promoter fragment containing only the TATA box and the nTPE site was used in a mobility shift assay. 50 ng of TPBF was incubated with the probe for 15 min, followed by the addition of either 50 or 100 ng of TBP and another 15-min incubation. There was no change between TPBF alone and TPBF incubated with TBP (Fig. 8, compare lane 2 with lanes 3 and 4), suggesting that TBP cannot bind to the TATA box when TPBF is bound to the nTPE.


Fig. 8. TPBF displaces TBP from the TATA box by binding to the nTPE. The order of the addition of TBP and TPBF and the amount added in mobility shift assays are indicated above each lane. The time interval between the additions was 15 min. Specific complexes are identified on the left. A diagram of the promoter fragment is shown at the bottom.
[View Larger Version of this Image (49K GIF file)]


Reversing the order of addition, we first incubated either 50 or 100 ng of TBP with the probe for 15 min and then added 50 ng of TPBF and incubated for another 15 min. In the absence of TPBF, a distinct complex generated by the binding of TBP to the TATA box was visible (Fig. 8, lanes 5 and 6). The complex completely disappeared with the addition of TPBF (Fig. 8, lanes 7 and 8). The absence of a supershifted complex suggests that the complex between TBP and the probe was disrupted by the binding of TPBF. These data indicate that TPBF, once bound to the nTPE, is able to prevent TBP binding and, furthermore, that it is able to disrupt the interaction between TBP and the TATA box.


DISCUSSION

We have shown that both activation and repression of the Acanthamoeba TBP gene promoter are mediated by the transcription factor TPBF. TPBF functions as an activator when bound to a cis-element located upstream of the TATA box. It functions as a repressor when bound to the lower affinity sequence located between the TATA box and the transcription initiation site. These observations permit construction of a general model describing how the cellular TBP level is controlled (Fig. 9). At a relatively low TPBF level, the TBP gene is highly expressed due to occupancy of the high affinity TPE. As the TPBF level increases, the lower affinity nTPE becomes occupied. This leads to a decrease in the rate of TBP gene expression by counteracting the effect of activating TPBF. If the TPBF level reaches a certain point, TBP gene expression can be completely shut off.


Fig. 9. Model of regulation of TBP gene expression. At a relatively low TPBF concentration, as shown at the top, the TBP gene is highly expressed due to TPBF occupancy of the high affinity TPE site. As the TPBF level increases, as shown at the bottom, the lower affinity nTPE site is occupied. This leads to a decrease of TBP gene expression by counteracting the effect of activating TPBF.
[View Larger Version of this Image (27K GIF file)]


In addition, these data show that the DNA sequence of the TPBF binding site determines whether bound TPBF can activate transcription. In this respect, TPBF is similar to MyoD, which also activates only when bound to particular DNA sequences (30). Although the mechanism is not clear, one can speculate that high affinity binding is necessary for activation. Alternatively, the propensity of the TPBF-bound DNA to bend or fail to bend (31) might determine whether the complex can activate transcription.

In contrast, all appropriately located TPBF-binding sequences can repress transcription. In the context of the TBP promoter, repression by TPBF is most likely caused by prevention of TBP binding to the TATA box or by displacement of prebound TBP. Both circumstances lead to inhibition of basal and activated transcription by preventing formation of the preinitiation complex. TPBF makes numerous contacts with the DNA-phosphate backbone (22). It is possible that these contacts might disrupt or prevent formation of the sharp DNA bend induced by TBP binding (32). However, in the context of the synthetic promoters, where the TPBF binding site is farther away from the TATA box, transcription away from the TPE or nTPE is not inhibited, but transcription toward the TPE is still inhibited (see Fig. 5). Here, it is not possible for TFIID to be displaced, but it is possible that the TPE or nTPE and bound TPBF are destabilizing one orientation of TFIID binding. This would thereby prevent the bidirectional transcription previously described for similar constructs or isolated TATA boxes (29). We note that in those promoters where the TPE is located further downstream from the TATA box, inhibition might result instead from a roadblock mechanism, although we have been unable to establish this point. In either case, displacement of TFIID binding is the most likely mechanism of repression in the context of the native TBP gene.

Transcription repression can be achieved by a variety of distinct mechanisms. Repressors can inhibit transcription of their target genes by inhibiting the functions of activators by simply competing for the binding sites on the promoters (33-35). Repressors can also inhibit transcription through down-regulation of the DNA-binding or transactivation activity of activators by forming complexes with them (36, 37). By targeting activators, these classes of repressors by and large only inhibit activated transcription. There is a third class of repressors that directly target a component(s) of the basal transcription machinery rather than inhibiting specific transcription activators. This class of repressor can destabilize or inhibit the formation of initiation complexes by targeting basal factors such as TFIID/TBP (38-41), TFIIB (5, 42), polymerase II (43), and TFIIE (44). For example, Dr1 prevents TFIIA and/or TFIIB from being recruited to the preinitiation complex by itself binding to TBP (40). The human cytomegalovirus immediate early protein 2 (IE2) (43) reportedly inhibits transcription by binding to an element located between the TATA box and the initiation site and blocking the recruitment of RNA polymerase II to the promoter. TPBF belongs to this third class of repressor. It functions by inhibiting the first essential step during transcription initiation, the association of TBP with the TATA box. While the exclusion of TBP from the TATA box is likely due to steric hindrance, it is somewhat surprising that TPBF is able to displace TBP from the TATA box. This mechanism of displacement gives TPBF the ability to dismantle an already assembled initiation complex and shut off TBP gene transcription completely, which might be necessary during some developmental stages in Acanthamoeba.

The ability of regulatory proteins to act as either repressors or activators depending on the context of their binding site relative to other promoter elements is emerging as an important theme in transcription regulation (45, 46). lambda  repressor provides an example of a phage repressor that, at low concentrations, can activate transcription from the cI promoter by binding to the OR1 and OR2 sites within the operator. At higher concentrations it inhibits transcription by binding to the lower affinity OR3 site in the operator (47). Indeed, since lambda  repressor does not bind cooperatively to OR3, this mechanism is strikingly analogous to that used within the Acanthamoeba TBP gene promoter.

In eukaryotic systems, several regulatory proteins can act as activators or repressors depending on context or concentration, such as herpes simplex virus ICP4 (5), Drosophila Kruppel (44, 48), the human Kruppel-related factor YY1 (8, 9), and p53 (49). Various mechanisms are involved in the action of these factors. For example, YY1 activates transcription by binding to the initiator sequence of the adenovirus P5 promoter (9), but YY1 can act as a repressor by binding to an upstream element (8). While the action of YY1 depends on the context of its binding sequence, Drosophila Kruppel determines activation or repression solely based on its concentration. Monomeric Kruppel interacts with TFIIB to activate transcription, whereas dimeric Kruppel, as a result of an increase in concentration, interacts with TFIIE to repress transcription (44). Our work with TPBF describes another protein of this type with additional novel features. TPBF appears to be the first eukaryotic transcription factor whose action is determined by both its concentration and the sequence context of its binding sites. TPBF binds to two structurally similar elements with different affinities. Our data show that a strong interaction between TPBF and its binding sites is required for transactivation, while only a modest interaction is sufficient for repression (Fig. 5). Although both TPBF-induced activation and repression are likely to be carried out through its interaction with basal factors, different mechanisms are clearly involved. While the mechanism of activation by TPBF is somewhat unclear, the close proximity of the nTPE to the TATA box suggests a mechanism for repression. Association between TBP and the TATA box, which is the first step during transcription initiation, is impeded by the binding of TPBF to the nTPE. Deciding between activation and repression as a consequence of the strength of interaction between transcription factors and their responding elements, as well as the location of these elements with respect to the TATA box could be a regulatory mechanism employed widely by cells.

In summary, we have described a eukaryotic promoter that is regulated in a fashion formally analogous to the lambda  repressor system. Since the rate of TBP gene expression is controlled by the cellular concentration of TPBF, it will also be of interest to determine how the level of TPBF is regulated, particularly since TPBF is likely to be involved in transcription of other genes, such as polyubiquitin (25). One working model is that TPBF gene expression is controlled at the basal level, specifically by the concentration of TBP. An increase in TBP concentration would stimulate TPBF transcription, which in turn would cause repression of the TBP gene by TPBF.


FOOTNOTES

*   This work was supported in part by National Eye Institute Grant EY 08706 (to E. B.) and a grant from the Lucille P. Markey Charitable Trust to the Department of Microbiology and Molecular Genetics, University of Vermont. The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Dagger    To whom correspondence should be addressed. Tel.: 802-656-8608; Fax: 802-656-8749; E-mail: ebateman{at}moose.uvm.edu.
1    The abbreviations used are: TF, transcription factor; TBP, TATA-binding protein; TPE, TBP promoter element; nTPE, negative TPE; TPBF, TBP promoter-binding factor; PCR, polymerase chain reaction; DTT, dithiothreitol.
2    W. Huang and E. Bateman, unpublished results.

Acknowledgment

We thank Dr. Tom Orfeo for critical comments on the manuscript.


REFERENCES

  1. Zawel, L., and Reinberg, D. (1995) Annu. Rev. Biochem. 64, 533-561 [CrossRef][Medline] [Order article via Infotrieve]
  2. Buratowski, S. (1994) Cell 77, 1-3 [Medline] [Order article via Infotrieve]
  3. Struhl, K. (1995) Annu. Rev. Genet. 29, 651-674 [CrossRef][Medline] [Order article via Infotrieve]
  4. Qasba, P., Lin, E., Zhou, M., Kumar, A., and Siddiqui, M. A. Q. (1996) Mol. Cell. Biol. 12, 1107-1116 [Abstract]
  5. Gu, B., Kuddus, R., and DeLuca, N. A. (1995) Mol. Cell. Biol. 15, 3618-3626 [Abstract]
  6. Lansford, R. D., McFadden, H. J., Siu, S. T., Cox, J. S., Cann, G. M., and Koshland, M. E. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 5966-5970 [Abstract]
  7. Jenkins, D. E., Martens, C. L., and Mocarski, E. S. (1994) J. Gen. Virol. 75, 2337-2348 [Abstract]
  8. Shi, Y., Seto, E., Chang, L.-S., and Shenk, T. (1991) Cell 67, 377-388 [Medline] [Order article via Infotrieve]
  9. Seto, E., Shi, Y., and Shenk, T. (1991) Nature 354, 241-245 [CrossRef][Medline] [Order article via Infotrieve]
  10. Hernandez, N. (1993) Genes & Dev. 7, 1291-1308 [CrossRef][Medline] [Order article via Infotrieve]
  11. Schultz, M. C., Reeder, R. H., and Hahn, S. (1992) Cell 69, 697-702 [Medline] [Order article via Infotrieve]
  12. Yamashita, S., Hisatake, K., Doi, K., Roeder, R. G., Horikoshi, M., and Nakatani, Y. (1993) Science 261, 463-466 [Medline] [Order article via Infotrieve]
  13. Comai, L., Tanese, N., and Tjian, R. (1992) Cell 68, 965-976 [Medline] [Order article via Infotrieve]
  14. Pugh, B. F., and Tjian, R. (1992) J. Biol. Chem. 267, 679-682 [Free Full Text]
  15. Kassavetis, G. A., Joazeiro, C. A. P., Pisano, M., Geiduschek, E. P., Colbert, T., Hahn, S., and Blanco, J. A. (1992) Cell 71, 1055-1064 [Medline] [Order article via Infotrieve]
  16. White, R. J., and Jackson, S. P. (1992) Cell 71, 1041-1053 [Medline] [Order article via Infotrieve]
  17. Wong, J., Liu, F., and Bateman, E. (1992) Gene (Amst.) 117, 91-97 [CrossRef][Medline] [Order article via Infotrieve]
  18. Chalut, C., Gallois, Y., Poterszman, A., Moncollin, V., and Egly, J. (1995) Gene (Amst.) 161, 277-282 [CrossRef][Medline] [Order article via Infotrieve]
  19. Horikoshi, M., Wang, C. K., Fujii, H., Cromlish, J. A., Weil, P. A., and Roeder, R. G. (1989) Nature 341, 299-303 [CrossRef][Medline] [Order article via Infotrieve]
  20. Sumita, K., Makino, Y., Katoh, K., Kishimoto, T., Muramatsu, M., Mikoshiba, K., and Tamura, T. (1993) Nucleic Acids Res. 21, 2769 [Medline] [Order article via Infotrieve]
  21. Liu, F., and Bateman, E. (1993) Nucleic Acids Res. 21, 4321-4329 [Abstract]
  22. Liu, F., and Bateman, E. (1994) J. Biol. Chem. 269, 18541-18548 [Abstract/Free Full Text]
  23. Huang, W., and Bateman, E. (1995) J. Biol. Chem. 270, 28839-28847 [Abstract/Free Full Text]
  24. Jeffrey, P. D., Gorina, S., and Pavletich, N. P. (1995) Science 267, 1498-1502 [Medline] [Order article via Infotrieve]
  25. Hu, Q., and Henney, R., Jr. (1996) Biochim. Biophys. Acta, in press
  26. Wong, J., Liu, F., and Bateman, E. (1992) Nucleic Acids Res. 20, 4817-4824 [Abstract]
  27. Ausubel, F. M., Brent, R., and Kingston, R. (1989) Current Protocols in Molecular Biology, Greene Publishing Associates and Wiley-Interscience, New York
  28. Wong, J., and Bateman, E. (1994) Nucleic Acids Res. 22, 1890-1896 [Abstract]
  29. Huang, W., Wong, J., and Bateman, E. (1996) Nucleic Acids Res. 24, 1158-1163 [Abstract/Free Full Text]
  30. Huang, J., Blackwell, T. K., Kedes, L., and Weintraub, H. (1996) Mol. Cell. Biol. 16, 3893-3900 [Abstract]
  31. Goodsell, D. S., Kaczor-Grzekowiak, M., and Dickerson, R. E. (1994) J. Mol. Biol. 239, 79-96 [CrossRef][Medline] [Order article via Infotrieve]
  32. Kim, Y., Geiger, J. H., Hahn, S., and Sigler, P. B. (1993) Nature 6446, 512-520
  33. Vincent, A. C., and Struhl, K. (1992) Mol. Cell. Biol. 12, 5394-5405 [Abstract]
  34. Kageyama, R., and Pastan, I. (1989) Cell 59, 815-825 [Medline] [Order article via Infotrieve]
  35. Schule, R., Umesono, K., Mangelsdorf, D. J., Bolado, J., Pike, J. W., and Evans, R. M. (1990) Cell 61, 497-504 [Medline] [Order article via Infotrieve]
  36. Ma, J., and Ptashne, M. (1987) Cell 50, 137-142 [Medline] [Order article via Infotrieve]
  37. Benezra, R., Davis, R. L., Lockshon, D., Turner, D. L., and Weintraub, H. (1990) Cell 61, 49-59 [Medline] [Order article via Infotrieve]
  38. Austin, R. J., and Biggin, M. D. (1995) Mol. Cell. Biol. 15, 4683-4693 [Abstract]
  39. Um, M., Li, C., and Manley, J. L. (1995) Mol. Cell. Biol. 15, 5007-5016 [Abstract]
  40. Inostroza, J. A., Mermelstein, F. H., Lane, W. S., and Reinberg, D. (1992) Cell 70, 477-489 [Medline] [Order article via Infotrieve]
  41. Fondell, J. D., Brunel, F., Hisatake, K., and Roeder, R. G. (1996) Mol. Cell. Biol. 16, 281-287 [Abstract]
  42. Baniahmad, A., Ha, I., Reinberg, D., Tsai, S., Tsai, M. J., and O'Malley, B. W. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 8832-8836 [Abstract]
  43. Lee, G., Wu, J., Luu, P., Ghazal, P., and Flores, O. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 2570-2575 [Abstract/Free Full Text]
  44. Sauer, F., Fondell, J. D., Ohkuma, Y., Roeder, R. G., and Jackle, H. (1995) Nature 375, 162-164 [CrossRef][Medline] [Order article via Infotrieve]
  45. Cowell, I. G. (1994) Trends Biochem. Sci. 19, 38-42 [CrossRef][Medline] [Order article via Infotrieve]
  46. Hanna-Rose, W., and Hansen, U. (1996) Trends Genet. 12, 229-234 [CrossRef][Medline] [Order article via Infotrieve]
  47. Ptashne, M. (1992) A Genetic Switch Phage Lamda and Higher Organisms, 2nd Ed., Cell Press and Blackwell Scientific Publications, Cambridge, MA
  48. Sauer, F., and Jackle, H. (1993) Nature 364, 454-457 [CrossRef][Medline] [Order article via Infotrieve]
  49. Kristjuhan, A., and Maimets, T. (1995) Eur. J. Biochem. 234, 827-831 [Abstract]

©1997 by The American Society for Biochemistry and Molecular Biology, Inc.