Characterization of Protein Kinase A and Protein Kinase C Phosphorylation of the N-Methyl-D-aspartate Receptor NR1 Subunit Using Phosphorylation Site-specific Antibodies*

(Received for publication, August 8, 1996, and in revised form, November 25, 1996)

Whittemore G. Tingley Dagger , Michael D. Ehlers Dagger , Kimihiko Kameyama Dagger , Carol Doherty Dagger , Janine B. Ptak , Clark T. Riley and Richard L. Huganir Dagger

From the Dagger  Department of Neuroscience,  Biopolymers Laboratory, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
Acknowledgments
REFERENCES


ABSTRACT

Modulation of N-methyl-D-aspartate receptors in the brain by protein phosphorylation may play a central role in the regulation of synaptic plasticity. To examine the phosphorylation of the NR1 subunit of N-methyl-D-aspartate receptors in situ, we have generated several polyclonal antibodies that recognize the NR1 subunit only when specific serine residues are phosphorylated. Using these antibodies, we demonstrate that protein kinase C (PKC) phosphorylates serine residues 890 and 896 and cAMP-dependent protein kinase (PKA) phosphorylates serine residue 897 of the NR1 subunit. Activation of PKC and PKA together lead to the simultaneous phosphorylation of neighboring serine residues 896 and 897. Phosphorylation of serine 890 by PKC results in the dispersion of surface-associated clusters of the NR1 subunit expressed in fibroblasts, while phosphorylation of serine 896 and 897 has no effect on the subcellular distribution of NR1. The PKC-induced redistribution of the NR1 subunit in cells occurs within minutes of serine 890 phosphorylation and reverses upon dephosphorylation. These results demonstrate that PKA and PKC phosphorylate distinct residues within a small region of the NR1 subunit and differentially affect the subcellular distribution of the NR1 subunit.


INTRODUCTION

Ionotropic glutamate receptors mediate most rapid excitatory transmission in the central nervous system and play important roles in synaptic plasticity, neuronal development, and neurological disorders (1-5). Glutamate receptors have been divided into NMDA1 (N-methyl-D-aspartate) and non-NMDA (kainate or AMPA) receptors based on their pharmacological and physiological properties (1, 2). Non-NMDA glutamate receptors activate and desensitize rapidly and mediate excitatory synaptic transmission. NMDA receptors are more slowly activated and desensitized and have a high Ca2+ permeability and a voltage-dependent Mg2+ block, two properties thought to underlie use-dependent synaptic plasticity in the brain (1-3). Molecular cloning studies have recently identified the genes encoding subunits for the NMDA and non-NMDA receptors (1, 2). NMDA receptors consist of two families of homologous subunits, the NR1 and NR2A-D subunits (6-9), and are thought to be pentameric or tetrameric complexes of the NR1 subunit with one or more of the NR2 subunits (10, 11). The differential expression of NR2 subunits in the various regions of the brain may account for the diversity of NMDA receptor subtypes (1). In addition, the NR1 subunit is highly alternatively spliced giving rise to at least seven forms of NR1 (NR1A-G) increasing the potential diversity of NMDA receptors in the brain (12-14).

Protein phosphorylation has been recognized as a major mechanism for the regulation of glutamate receptor function (15). NMDA receptors appear to be regulated by a number of protein kinases and phosphatases. Activation of protein kinase C (PKC) by phorbol esters have been demonstrated to activate (16, 17) or depress (18, 19) neuronal NMDA receptors. In addition, intracellular perfusion of purified PKC into spinal trigeminal neurons potentiates NMDA receptors (20), and PKC seems to mediate the modulation of NMDA receptors by µ-opioids in these cells (21). Activation of PKC by phorbol esters can also potentiate recombinant NMDA receptors expressed in Xenopus oocytes including homomeric NR1 receptors (13, 22, 23) and heteromeric receptors consisting of the NR1 subunit coexpressed with the NR2A or NR2B subunits (8, 24). In addition, recent studies in rat hippocampal neurons have suggested that cAMP-dependent protein kinase can also potentiate NMDA receptors (25).

Biochemical studies have demonstrated that NMDA receptors are directly phosphorylated by protein kinase C and protein tyrosine kinases (26-28). PKC has been shown to phosphorylate the NR1 subunit in neurons and in heterologous expression systems (26). This phosphorylation occurs on the C terminus of the NR1 subunit, and mutation of four serine residues in the C terminus dramatically reduce PKC phosphorylation of NR1 (26). These four serine residues are all contained within a single exon (C1) which is regulated by alternative splicing of NR1 mRNA (12, 13). Interestingly, the presence of the C1 exon induces the NR1 subunit to cluster into receptor-rich domains associated with the cell surface when expressed in heterologous cells, and PKC phosphorylation of the NR1 subunit disrupts these spontaneous aggregates of the receptor (29).

In the present study we have further characterized the phosphorylation of the NR1 protein in vitro and in situ using site-specific mutagenesis and phosphorylation site-specific antibodies. These phosphorylation site-specific antibodies recognize the NR1 protein only when specific serine residues are phosphorylated. We show that in addition to PKC, PKA also phosphorylates the C terminus of NR1. PKC specifically phosphorylates threonine 879 and serines 890 and 896, whereas PKA phosphorylates serine 897. Moreover, only phosphorylation of serine 890 by PKC induces the dispersal of the clusters of the NR1 subunit in fibroblasts, suggesting that PKC and PKA may differentially regulate NR1 subunits in the brain.


EXPERIMENTAL PROCEDURES

Materials

NR1A cDNA was a generous gift of S. Nakanishi. PKC and the catalytic subunit of PKA were purified from rat brain and bovine heart, respectively, as described (30, 31). Other materials were purchased from the following sources: radioisotopes, DuPont NEN; phorbol esters and forskolin, Calbiochem; cellulose TLC (thin layer chromatography) plates and XAR autoradiography film, Kodak; PVDF membrane, Millipore; nitrocellulose membrane, VWR; tissue culture media, sera, and supplies, Life Technologies, Inc. All other chemicals, unless otherwise noted, were from Sigma.

Synthesis of Phosphopeptides

Peptides were synthesized using Applied Biosystems 430A Peptide Synthesizer using the FastMoc procedures and reagents supplied by the Perkin-Elmer, Applied Biosystems division. Di-tert-butyl-N,N-diisopropylphosphoramidite, FMOC serine (hydroxyl group not protected), and FMOC threonine (hydroxyl group not protected) were obtained from Novabiochem, La Jolla, CA. At each amino acid position where phosphorylation was desired, the FMOC serine without hydroxyl group protection was substituted using empty cartridges of the same amino acid and the same reaction vessel cycles as would have been used for the normal residue. The N-terminal residue was added as the t-butoxycarbonyl protected amino acid rather than the FMOC derivative so as to avoid the presence of a free amino group in subsequent reactions. A portion of the resin-bound unprotected pre-phosphorylated peptide may be removed at this point to produce the non-phosphorylated peptide by the normal methods of peptide cleavage and deprotection.

Resin-bound peptide (330 mg) and a magnetic stir bar were placed in an Applied Biosystems peptide synthesizer reaction vessel which was modified so that one cap did not have the drainage hole. Into the vessel was placed 1 g of di-tert-butyl-N,N-diisopropylphosphoramidite and 0.6 g of 1-H-tetrazole for each unprotected serine or threonine in the peptide and 3.7 ml of N,N-dimethylacetamide. A normal reaction vessel filter and normal cap were secured on the top of the reaction vessel, and the mixture was allowed to stir at room temperature for 3-5 h.

When the reaction was complete, the vessel was removed from the magnetic stirrer and inverted over a filter flask. The blank end cap was removed, and the reaction mixture was drained away from the resin. The resin was washed three times with 10-ml portions of N,N-dimethylacetamide and then with three 10-ml portions of diethyl ether and dried overnight under vacuum.

The resin-bound intermediate was weighed to confirm weight gain. The blank plug was replaced and the vessel was again placed on the stir plate. To the resin was added 1 ml of t-butylhydroperoxide and 3 ml of anhydrous N,N-dimethylformamide. The reaction vessel was resealed, and the mixture was allowed to stir at room temperature for 3-5 h. At the end of this time the vessel was inverted over a filter flask, and the oxidation mixture was drained away from the resin. The resin was then washed with three 5-ml portions each of t-amyl alcohol, N,N-dimethylacetamide, once with 5 ml of acetic acid, three times with t-amyl alcohol, and methanol. Finally, the resin was washed with three 10-ml portions of diethyl ether. The rinsed resin was dried under vacuum, still in the same reaction vessel. The dried resin was weighed to confirm weight gain.

To the resin-bound phosphopeptide in the reaction vessel was added 9.5 ml of trifluoroacetic acid, 0.25 ml of water, 0.25 g of thiocresol, and, in the case of arginine-containing peptides, 0.1 ml of thioanisole. The reaction vessel was sealed and the mixture was stirred for 3 h. After 3 h, the reaction mixture was filtered through a filter flask containing about 100 ml of cold methyl t-butyl ether. The resulting suspension was separated in 50-ml conical tubes. The mixture was centrifuged at 2000 rpm for 10 min; the supernatant was decanted, and the pellet was resuspended in fresh methyl t-butyl ether. This was repeated three more times. The precipitate was finally dissolved in 200 ml of 0.1% v/v trifluoroacetic acid in water, and the clear solution was shell-frozen and lyophilized. The peptide was subsequently purified by reverse-phase high performance liquid chromatography. TOF MALDI mass spectrometry of the purified peptides were performed using a Kompact MALDI III (Manchester, United Kingdom) in the Middle Atlantic Mass Spectrometry Laboratory, located at the Johns Hopkins University School of Medicine.

Generation of Anti-phosphopeptide Antibodies

The peptides KTSTLASSFKRRR and KFKRRRSSKDTST, corresponding to amino acids 884-895 and amino acids 891-902 of NR1A, respectively, were synthesized as described above. Lysine residues were included in the N-terminal positions of the peptides to facilitate glutaraldehyde coupling to carrier protein, thyroglobulin. During synthesis, FMOC unprotected serine residues (Nova Biochemicals) were included in positions 889, 890, 896, and/or 897. The resulting phosphopeptides were coupled to thyroglobulin and used to immunize New Zealand White rabbits, and sera were obtained periodically by Hazleton Research Products. Polyclonal anti-phosphopeptide antibodies were purified from sera by sequential chromatography on Affi-Gel 15 (Bio-Rad) columns covalently linked to unphosphorylated and phosphorylated peptides. Antibodies were eluted from the peptide affinity columns using 100 mM glycine (pH-2.7). Anti-NR1 C-terminal antibodies were described previously (26).

NR1 Fusion Protein Phosphorylation

Bacterial fusion proteins containing the C-terminal region of NR1A (amino acids 834-938) were constructed by polymerase chain reaction amplification of NR1A cDNA (primers 5'-ACCATGGATCCCGAGATCGCCTACAAGCGA-3' and 5'-ACCATGAATTCCTCAGCTCTCCCTATGACG-3'), subcloning the polymerase chain reaction products into restriction endonuclease sites (BamHI and EcoRI) in the pTrcHis vector (Invitrogen), and transforming BL21 Escherichia coli. Transformed bacteria were grown in 400-ml cultures, induced with isopropyl-1-thio-beta -D-galactopyranoside (10 mM), lysed in guanidinium (6 M), and fusion proteins purified on a Ni2+-NTA-agarose column (Qiagen) in the presence of 8 M urea, and dialyzed against Tris buffered-saline or phosphate-buffered saline (PBS), according to Invitrogen's protocol. For phosphorylation reactions, 1 µg of purified fusion protein was incubated with 20 ng of bovine cardiac PKC and/or 100 ng of rat brain PKA (catalytic subunit), or no kinase, for 30 min at 30 °C in 100 µl total volume. Phosphorylation reaction buffer contained 50 mM Hepes (pH 7.4), 10 mM MgCl2, 1 mM CaCl2, 50 µM ATP, 50 µg/ml phosphatidylserine, and 5 µg/ml diacylglycerol. 5 µCi of [gamma -32P]ATP was included. In most cases, fusion proteins were resolved by SDS-PAGE using 14% polyacrylamide gels and either excised from the gel for phosphopeptide mapping or transferred to PVDF membrane by electrophoresis at 30 V. In some cases, fusion protein was applied directly to nitrocellulose membranes ("slot blots"), using a Bio-Dot SF apparatus (Bio-Rad) according to the manufacturer's protocol.

Phosphopeptide Mapping

Polyacrylamide gel fragments containing radiolabeled, phosphorylated NR1 C-terminal fusion protein were incubated with 0.3 mg/ml trypsin (Sigma) in 1 ml of 0.4% NH4HCO3 for 20 h at 37 °C. After removal of NH4HCO3 by lyophilization, phosphopeptides were resuspended in 10 µl of H2O. The tryptic digests were spotted onto TLC plates (Kodak) and separated by electrophoresis (500 V, horizontal) in the presence of acetic acid/pyridine/H2O, 19:1:89 (v/v), and ascending chromatography in pyridine/butanol/acetic acid/H2O, 15:10:3:12 (32). Phosphopeptides were visualized by autoradiography.

Immunoblotting

PVDF or nitrocellulose membranes were fixed in acetic acid/methanol/H2O, 10:25:65 (v/v), blocked (1 h) with 0.5% nonfat dry milk (Carnation) and 0.1% Tween 20, incubated (1 h) with primary antibodies (150-500 ng/ml in blocking buffer, washed (5 × 5 min, blocking buffer), and incubated (1 h) with horseradish peroxidase-conjugated anti-rabbit Ig (1:5,000 in blocking buffer; Amersham Corp.). After final washes in Tris-buffered saline (5 × 5 min) membranes were immersed in chemiluminescence detection reagent (DuPont NEN) for 1 min and then exposed to XAR film. Exposure time ranged from 10 s to 10 min.

Cell Culture and Transfection

Quail fibroblast (QT-6) cells or human embryonic kidney-293 (HEK) cells were maintained at 37 °C under 5% CO2 and passaged every 4-6 days. QT-6 cell medium contained 199 Earles medium with 5% fetal bovine serum, 10% tryptose phosphate broth, and 1% dimethyl sulfoxide (Sigma). HEK cell medium included minimal essential media with 10% fetal bovine serum, 100 units/ml penicillin, and 100 µg/ml streptomycin. For transfections, the pRK5 expression vector containing NR1 cDNA was precipitated onto cultured cells by the calcium phosphate method (33) using 20 µg of DNA per 10-cm culture dish (1 × 106 cells). After incubation for 8-12 h under 3% CO2, remaining DNA was washed away with fresh media and cells were returned to 5% CO2. For immunocytochemical experiments, cells were immediately replated onto poly-lysine coated glass coverslips. Thirty to forty-five hours after transfection, cells were treated with 100 nM PMA, 20 µM forskolin, or vehicle solution, rinsed in PBS, and either fixed in 4% paraformaldehyde, or solubilized in SDS gel sample buffer and proteins resolved on 8% polyacrylamide gels.

Immunocytochemistry

QT-6 cells were fixed with 4% paraformaldehyde, 4% sucrose in PBS for 10 min, and blocked with 6% bovine serum albumin, 0.5% Triton X-100, PBS for 1 h. Primary antibody (30 ng/ml) was applied overnight at 4 °C in 1% bovine serum albumin, 0.5% Triton X-100, PBS and then washed away with three buffer changes over 30 min. Fluorescein (fluorescein isothiocyanate)-conjugated donkey anti-rabbit IgG (1:400; Jackson ImmunoResearch) secondary antibody was applied in the same buffer for 1 h at room temperature. After three more washes, cells were immersed in permafluor solution (Immunon) supplemented with 2.5% 1,4-diazabicyclo[2.2.2]-octane (Aldrich) and mounted on glass slides. Fluorescence was visualized and quantified using a Zeiss Axiophot microscope. In some cases, 1-2-µm thick optical sections were obtained in the x-y plane by scanning laser confocal microscopy using an argon laser and the Bio-Rad MRC-600 confocal microscope system. Excitation was at 488 nm, and emissions were taken between 510-515 nm. Confocal images were retained using COMOS version 6.03 software (Bio-Rad) and processed with Adobe Photoshop and ClarisDraw software.

Hippocampal Slice Preparation

The hippocampi of 6-8-week-old male Sprague-Dawley rats were dissected and chilled in ice-cold ACSF solution (126 mM NaCl, 5 mM KCl, 1.25 mM NaH2PO4, 25 mM NaHCO3, 10 mM D-glucose, 2 mM CaCl2, 2 mM MgCl2, saturated with 95% O2/5% CO2). Thick slices (400 µM) were prepared using a tissue chopper and incubated in an interface chamber for 60 min at room temperature. Eight to ten slices were transferred to a 35-mm diameter dish containing 1 ml of ACSF solution in a humidified 95% O2/5% CO2 gas saturated chamber. After 20 min incubation, slices were treated with 100 nM PMA and 20 µM forskolin for 15 min and then the slices were transferred in 5 ml of ice-cold ACSF solution to wash out the drug. After ACSF solutions were discarded, the slices were sonicated on ice with 1 ml of buffer A (10 mM Na3PO4 (pH 7.0), 100 mM NaCl, 5 mM EDTA, 5 mM EGTA, 1 mM Na3VO4, 10 mM Na4P2O7, 50 mM NaF, 10 units/ml trasylol) for 30 s. The homogenates were centrifuged at 12,000 × g for 5 min. After discarding the supernatant, the membrane pellet was resuspended in SDS sample buffer.


RESULTS

Phosphorylation of NR1 Protein by PKA and PKC

In order to identify kinases that directly phosphorylate the NR1 subunit, bacterial fusion proteins containing the C-terminal region of NR1 protein were incubated with purified kinases in the presence of [gamma -32P]ATP (Fig. 1). Previous results indicated that PKC phosphorylates the NR1 subunit in transfected cells and that mutation of four serine residues (serines 889, 890, 896, and 897) eliminated the majority of PKC phosphorylation (26). Consistent with these findings, PKC rapidly phosphorylated the C-terminal region of the NR1 subunit (Fig. 1C), with mutation of serine residues 889, 890, 896, and 897 to alanine dramatically reducing PKC phosphorylation (data not shown). Interestingly, based on the characterized consensus sequence of PKA substrates (34), the C-terminal region of the NR1 subunit also contains a potential site for PKA phosphorylation (serine 897). Indeed, PKA readily phosphorylated the NR1 C-terminal fusion protein (Fig. 1, C and D). Mutation of serine residues 896 and 897 to alanines (S896A, S897A) completely eliminated PKA phosphorylation of the fusion protein (Fig. 1D).


Fig. 1. PKA and PKC phosphorylation of the C-terminal region of NR1. A, schematic of the C-terminal region of NR1, including the fourth transmembrane domain (TMIV), the regions encoded by two alternatively spliced exons (CI and CII), and the protein sequence of the C1 exon. Serine and threonine residues subjected to site-specific mutagenesis are indicated by their residue numbers. B, schematic of a bacterial fusion protein containing amino acids 834-938 of NR1 protein. C, the wild-type fusion protein shown in B was incubated without kinase or with purified PKA, PKC, or both in the presence of [gamma -32P]ATP for 30 min at 30 °C and resolved by SDS-PAGE and analyzed by autoradiography. D, PKA was incubated with wild-type fusion protein or a mutant fusion protein in which serine residues 896 and 897 had been converted to alanine residues (S896A, S897A) and then analyzed by SDS-PAGE and autoradiography.
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In order to characterize PKA phosphorylation of the NR1 subunit, tryptic phosphopeptide map analysis was performed on C-terminal NR1 fusion proteins. Fusion proteins were phosphorylated in vitro by PKA and/or PKC, resolved by SDS-PAGE, and visualized by autoradiography. The phosphorylated NR1 fusion protein was excised from the SDS-PAGE gel, proteolyzed with trypsin, and the resulting tryptic phosphopeptides resolved by two-dimensional thin layer chromatography and visualized by autoradiography (see "Experimental Procedures"). PKA phosphorylation of the fusion protein produced phosphopeptides that differed markedly from those produced by PKC phosphorylation (compare Fig. 2, A and B). However, tryptic phosphopeptides generated from fusion protein that had been phosphorylated by both PKA and PKC (Fig. 2C) closely resembled tryptic phosphopeptides of the NR1 subunit isolated from neurons in culture as indicated by the numbered phosphopeptides (compare with Fig. 2 from Ref. 26).


Fig. 2. Phosphopeptide map analysis of tryptic phosphopeptides from phosphorylated NR1 fusion protein. Phosphorylated NR1 fusion proteins were excised from gels similar to those in Fig. 1, digested with trypsin, and the resulting tryptic phosphopeptides spotted onto thin layer chromatography plates and resolved in two dimensions by electrophoresis (horizontal) and ascending chromatography (vertical) as described under "Experimental Procedures." Tryptic phosphopeptides detected by autoradiography were numbered in accordance with similar phosphopeptides isolated from full-length NR1 protein phosphorylated in situ in neurons (see Tingley et al. (26)). Open circles indicate the origin. Phosphopeptide map analyses are shown for wild-type NR1 fusion protein (A-C) or mutant (S896A,S897A) fusion protein (D) phosphorylated with PKA (A), PKC (B), or both (C + D).
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To associate the tryptic phosphopeptides with specific residues in the NR1 protein, tryptic phosphopeptide maps of mutated NR1 C-terminal fusion proteins were obtained. Conversion of serine residues 896 and 897 in the NR1 fusion protein to alanine residues resulted in tryptic phosphopeptide maps of PKC and PKA phosphorylated fusion protein that lacked phosphopeptides 1,4,4',6 and 6' (Fig. 2D). Similarly, phosphopeptide maps of a mutant fusion protein where serine residues 889 and 890 were mutated to an alanine residue lacked phosphopeptides 3, 5, and 8, whereas mutation of threonine 879 to alanine eliminated phosphopeptide 2 (see Table I). Analogous mutants, expressed as full-length recombinant NR1 proteins in human embryonic kidney-293 (HEK) cells and phosphorylated in situ, yielded results virtually identical to those seen in vitro (data not shown). These results demonstrate that the majority of NR1 protein phosphorylation occurs on two pairs of serine residues (889-890, 896-897) in the C-terminal region of NR1 (see Fig. 1A). PKC phosphorylates both pairs of serines, whereas PKA phosphorylates only the second pair (896-897). PKC also phosphorylates threonine 879. In an attempt to determine which specific serine residues in the two pairs of serines are phosphorylated by the two kinases, we examined fusion proteins in which individual serines were converted to alanines. However, mutation of individual serines had little effect on phosphorylation of the mutant fusion proteins by PKA and PKC (data not shown) suggesting that both serines in each pair could be phosphorylated by the appropriate enzymes in these mutants. However, we were concerned that these results may not indicate which serine residues are phosphorylated by these kinases in the wild-type NR1 subunit. We therefore turned to phosphorylation site-specific antibodies to examine the physiological phosphorylation sites of NR1.

Table I.

Phosphopeptide identification


Mutations Missing phosphopeptides

S896A, S897A 1, 4, 4', 6, 6'
S889A, S890A 3, 5, 8 
T879A 2

Phosphorylation Site-specific Antibodies for Serine Residues 890, 896, and 897

Phosphorylation site-specific antibodies has previously been used to examine the phosphorylation of individual proteins in vivo (35-41). We therefore attempted to generate phosphorylation site-specific antibodies that would specifically recognize the various phosphorylated forms of the NR1 subunit. Synthetic peptides corresponding to sequence of NR1 surrounding the pairs of serines were synthesized, and the peptides were chemically phosphorylated on serine residues 889, 890, 896, or 897 (see "Experimental Procedures"). These peptides were then used to immunize rabbits, and the resulting antibodies were affinity purified on phosphopeptide affinity columns.

The specificity of the phosphorylation site-specific antibodies was characterized using immunoblots of the NR1 fusion protein. Because of its bacterial origin, the fusion protein should have a minimal level of basal phosphorylation. Appropriately, the anti-phosphoserine 890, anti-phosphoserine 896, and anti-phosphoserine 897 antibodies only poorly recognized NR1 fusion protein (Fig. 3, A-C). In contrast, phosphorylation of the NR1 fusion protein with PKA markedly increased its recognition by the anti-phosphoserine 897 antibody (Fig. 3C), whereas phosphorylation of the NR1 fusion protein with PKC markedly increased its recognition by the anti-phosphoserine 890 and anti-phosphoserine 896 antibodies (Fig. 3, A and B). The anti-phosphoserine 889 antibody recognized both unphosphorylated and phosphorylated fusion protein equally well and was not investigated further (data not shown). These results indicate that the anti-phosphoserine 890, anti-phosphoserine 896, and anti-phosphoserine 897 antibodies recognize NR1 protein only when it is phosphorylated. In addition, these results suggest that PKA phosphorylates serine 897 and PKC phosphorylates serines 890 and 896. 


Fig. 3. Specificity of phosphorylation site-specific antibodies for phosphorylated NR1 fusion protein. Synthetic peptides corresponding to phosphorylation sites on NR1 protein were chemically phosphorylated and used to generate polyclonal antibodies. A-C and E, wild-type NR1 fusion protein was phosphorylated in the presence of unlabeled ATP, resolved by SDS-PAGE, transferred to PVDF membrane, and immunoblotted with phosphorylation site-specific antibodies generated against the phosphoserine residue(s) 890 (A), 896 (B), or 897 (C) or 896 and 897 (E). D, phosphorylation reactions containing wild-type fusion protein and no kinase, PKA, PKC, or both, were incubated for 30 min. In some reactions PKA or PKC were added sequentially, as indicated, and incubated for another 30 min, then proteins were applied directly to nitrocellulose membranes using a "slot-blot" device, and immunoblotted with phosphorylation site-specific antibodies.
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Intriguingly, although the anti-phosphoserine 896 antibody readily recognized the NR1 fusion protein after PKC phosphorylation, it reacted poorly after combined PKC and PKA phosphorylation (Fig. 3B). One possible explanation of this result is that PKA phosphorylation of serine 897 inhibits PKC phosphorylation at serine 896. Alternatively, phosphorylation at both serines 896 and 897 may simply reduce recognition of phosphoserine 896 by the anti-phosphoserine 896 antibody. To test these two possibilities the order of addition of PKA and PKC to the phosphorylation reaction was varied. Interestingly, even when NR1 fusion protein was incubated first with PKC for 30 min before addition of PKA, the immunoreactivity of the anti-phosphoserine 896 antibody decreased (Fig. 3D). This result indicates that PKA phosphorylation of serine 897 inhibits recognition of phosphoserine 896 by the anti-phosphoserine 896 antibody. To directly examine whether serines 896 and 897 could be simultaneously phosphorylated, antisera against a synthetic NR1 phosphopeptide in which serine residues 896 and 897 were both chemically phosphorylated was generated. In immunoblot experiments, this antibody strongly recognized the NR1 fusion protein after preincubation with both PKC and PKA and only weakly recognized the NR1 fusion protein which was phosphorylated by either kinase alone (Fig. 3E). These findings showed that serines 896 and 897 could be simultaneously phosphorylated by two distinct protein kinases.

Using the phosphorylation site-specific antibodies, we examined the phosphorylation of the full-length NR1 protein expressed in cultured cells. HEK cells were transiently transfected with NR1 cDNA and treated with combinations of two kinase activators, PMA (phorbol 12-myristate 13-acetate), a phorbol ester that stimulates PKC, and forskolin, which stimulates PKA indirectly by increasing the production of cAMP by adenylyl cyclase. In immunoblots of total cell lysates, each of the phosphorylation site-specific antibodies recognized a single 120-kDa protein (Fig. 4, A-D) that comigrated with the NR1 subunit (Fig. 4E). Mutation of the appropriate serine residues in the NR1 subunit completely eliminated the recognition of the NR1 subunit by the relevant phosphorylation site-specific antibody (data not shown). Treatment of HEK cells expressing wild-type NR1 protein with PMA increased the recognition of the NR1 subunit by the anti-phosphoserine 890 and the anti-phosphoserine 896 antibodies, whereas prior forskolin treatment had no effect on immunorecognition of the NR1 subunit by these antibodies (Fig. 4, A and B). In contrast, forskolin treatment, but not PMA treatment, increased the binding of the anti-phosphoserine 897 antibody to the NR1 subunit (Fig. 4C). Finally, the anti-phosphoserines 896/897 antibody gave a strong signal primarily after combined PMA and forskolin treatment of transfected cells (Fig. 4D). Immunoblots using a phosphorylation state-independent antibody against the C terminus of NR1 indicated that the amount of NR1 protein was not affected by either PMA or forskolin treatment (Fig. 4E). The detection of the phosphorylated NR1 subunit was completely eliminated when the antibody was incubated with the phosphorylated peptide used as immunogen but not by the non-phosphorylated peptide (data not shown).


Fig. 4. Immunoblots of the recombinant NR1 subunit phosphorylated in situ with phosphorylation site-specific antibodies. Human embryonic kidney 293 (HEK) cells expressing wild-type NR1A were treated with control solution (basal), forskolin (20 µM), phorbol 12-myristate 13-acetate (TPA) (100 nM), or both for 30 min, and solubilized in SDS sample buffer. Total cellular proteins (~10 µg/lane) were resolved by SDS-PAGE and transferred to nitrocellulose and immunoblotted with the anti-phosphorylation site-specific antibodies generated against phosphoserine(s) 890 (A), 896 (B), 897 (C), 896/897 (D), or an antibody to the C terminus of NR1 protein (E).
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To investigate the phosphorylation of the NR1 subunit in the brain, hippocampal slice homogenates were subjected to immunoblot analysis using the phosphorylation site-specific antibodies. In contrast to transfected cells, the phosphorylation site-specific antibodies cross-reacted with proteins in the total homogenates. However, these cross-reacting proteins could be minimized by isolating membrane preparations from the hippocampal slices (data not shown). In the isolated membrane preparations from acute slices of adult rat hippocampus, the phosphorylation site-specific antibodies recognized a 120-kDa protein that comigrated with the NR1 subunit, indicating that these sites are phosphorylated in vivo (Fig. 5). Phorbol ester treatment of the hippocampal slices increased the immunorecognition of NR1 by the anti-phosphoserine 890, the anti-phosphoserine 896, and the anti-phosphoserines 896/897 antibodies but had no effect on the signal detected by the anti-phosphoserine 897 antibody. However, forskolin treatment increased the immunorecognition of NR1 by both the anti-phosphoserine 897 antibody and the anti-phosphoserine 896/897 antibody. Taken together, these data demonstrated that the NR1 subunit is basally phosphorylated on serine residues 890 and 897 in untreated hippocampal slice preparations and has a lower basal phosphorylation of serine 896. Phorbol ester treatment increased the phosphorylation of serine 896 and caused serines 896 and 897 to become phosphorylated simultaneously. In addition, forskolin treatment increased both the phosphorylation of serine 897 and the simultaneous phosphorylation of 896 and 897. The detection of the phosphorylated NR1 subunit was completely eliminated when the antibody was incubated with the phosphorylated peptide used as immunogen but not by the non-phosphorylated peptide (data not shown).


Fig. 5. Phosphorylation of the NR1 subunit in hippocampus in vivo detected using phosphorylation site-specific antibodies. Immunoblot analysis of the phosphorylation of NR1 in hippocampal slice preparations. Membrane preparations (50 µg) from hippocampus were immunoblotted with phosphorylation site-specific antibodies against phosphoserine 890 (A), phosphoserine 896 (B), phosphoserine 897 (C), and phosphoserine 896 and 897 (D). The slices were incubated for 15 min with control solution (basal) or with phorbol 12-myristate 13-acetate (TPA) (100 nM) or forskolin (20 µM) or TPA + forskolin. Upper inset (described as alpha  NR1 C terminus) shows the same blot reprobed with a phosphorylation state-independent C-terminal NR1 antibody after stripping the phosphorylation site-specific antibodies.
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Regulation of the Subcellular Distribution of NR1 Protein by PKC and PKA Phosphorylation

In addition to the phosphorylation sites, the C1 exon cassette in the C-terminal region of the NR1 subunit contains molecular cues that regulate its subcellular targeting in transfected cells (29). NR1 subunits containing the C1 cassette spontaneously aggregate into receptor-rich domains near the surface of these cells, whereas NR1 splice variants that lack the cassette do not form these receptor-rich domains and are diffusely distributed throughout the cells. We have previously shown that phorbol ester treatment of QT6 cells expressing the NR1 subunits containing the C1 exon disrupts the NR1-rich domains resulting in a diffuse distribution of the NR1 subunits similar to that seen with NR1 splice variants missing the C1 exon. This phorbol ester-induced redistribution of the NR1 subunit is due to the phosphorylation of serine residues within the C1 cassette (29). Indeed, mutation of serines 889, 890, 896, and 897 to alanine residues abolishes both the vast majority of PKC phosphorylation and eliminates the phorbol ester-induced redistribution of the NR1 subunit (26, 29). These results suggest that the C1 cassette interacts with cytoskeletal proteins and that this interaction is disrupted by phosphorylation of the NR1 subunit.

In order to further investigate the effects of phosphorylation on the targeting of the NR1 subunit, we examined the subcellular distribution of wild-type and mutant NR1 proteins in transfected QT6 cells treated with kinase activators. The QT-6 cell line has been used as a model system for studying the clustering of neurotransmitter receptors such as the nicotinic acetylcholine receptor (42) and the NMDA receptor (29). To determine which serine was necessary and sufficient for the phorbol ester-induced redistribution of the NR1 subunit, four different NR1 mutants were examined where three out of the four serines were converted to alanine residues. None of these mutations had any effect on the formation of NR1-rich domains in the absence of phorbol ester treatment (Fig. 6A). However, mutation of serines 890, 896, and 897 (NR1-SAAA), serines 889, 890, and 897 (NR1-AASA), or serines 889, 890, and 896 (NR1-AAAS) to alanines completely abolished the phorbol ester-induced redistribution of the NR1 clusters (Fig. 6A). In contrast, mutation of serines 899, 896 at 897 (NR1-ASAA) to alanine residues did not inhibit the PKC phorbol redistribution of NR1 site domains. These findings indicated that the subcellular redistribution of NR1 caused by PMA treatment was specifically due to PKC phosphorylation of serine 890 and not the neighboring serines 889, 896, or 897. Consistent with these results, immunostaining and confocal microscopy using the anti-phosphoserine 890 antibody revealed an intracellular pool of phosphorylated NR1 protein present around the nucleus of phorbol ester-treated cells but not untreated cells (Fig. 6B). Virtually identical regulation of the subcellular distribution of the NR1 subunit occurred in HEK cells transfected with NR1 cDNA (data not shown).


Fig. 6. Protein phosphorylation of serine 890 regulates the subcellular distribution of NR1 protein. Quail fibroblast (QT6) cells were transfected with wild-type NR1A cDNA (wt NR1A) or mutant NR1A cDNA in which serine residues 890, 896, and 897 (NR1-SAAA), serines 889, 896, and 897 (NR1-ASAA), serines 889, 890, and 897 (NR1-AASA), or serines 889, 890, and 896 (NR1-AAAS) were mutated to alanines. The cells were treated for 30 min with either control solution (left panels) or 100 nM phorbol 12-myristate 13-acetate (TPA) (right panels) immediately before fixation and staining with either anti-NR1 C-terminal antibody and rhodamine-conjugated secondary antibody (A) or anti-phosphoserine 890 antibody and fluorescein isothiocyanate-conjugated secondary antibody (B). Stained cells were then visualized by either immunofluorescent microscopy (A) or scanning laser confocal microscopy (B). Bars, 10 µm.
[View Larger Version of this Image (70K GIF file)]


Examination of the time course of NR1 protein phosphorylation and redistribution was examined in QT6 cells expressing wild-type NR1 protein indicated that phosphorylation of serine 890 increased within 5 min of PMA treatment (Fig. 7A), and the level of NR1 protein did not change for up to 6 h of PMA treatment (Fig. 7B). Quantification of NR1 distribution using immunofluorescence microscopy revealed that the number of cells containing NR1-enriched domains began to decrease within 5 min of PMA treatment and were virtually absent within 20 min (Fig. 7C). This phorbol ester-induced redistribution of NR1 was reversible. Treatment of NR1 expressing cells with a water-soluble phorbol ester, phorbol 12,13-diacetate (PDA), followed by washout of the phorbol ester, resulted in the reaggregation of the NR1 subunit over several minutes (Fig. 7D). In contrast, forskolin treatment had no effect on NR1-enriched domains (Fig. 7E), despite an increase in phosphorylation of serine 897 as revealed by the anti-phosphoserine 897 antibodies (data not shown). These findings indicate that the NR1 subunit rapidly and reversibly redistributes between subcellular compartments in response to phosphorylation of serine 890. 


Fig. 7. Time course of NR1 protein phosphorylation and redistribution after PKC and PKA activation. A-C, QT-6 cells expressing wild-type NR1 cDNA were treated with PMA for varying lengths of time and immediately solubilized in SDS gel sample buffer (A and B) or fixed in paraformaldehyde (C). Total proteins were immunoblotted with anti-NR1 C-terminal antibody (A) or anti-phosphoserine 890 antibody (B). C, cells were stained with anti-NR1 C-terminal antibody and fluorescein (fluorescein isothiocyanate)-conjugated secondary antibody, and immunofluorescence microscopy was used to quantitate transfected cells with diffuse intracellular staining (circles), NR1 enriched domains (squares), or both (diamonds). The percentage of transfected cells examined (160-180 cells) at each time point are plotted as a function of the length of PMA treatment. Data are from a typical experiment. D, cells were treated with the water-soluble phorbol ester PDA for 30 min and then the phorbol ester was washed out. At various times after removal of PDA, the cells were fixed and immunofluorescence microscopy was used to quantitate the number of cells containing diffuse intracellular staining, NR1-enriched domains, or both. E, QT-6 cells expressing wild-type NR1 cDNA were treated with control solution, PMA, or forskolin for 30 min, fixed, immunostained, and quantitated for NR1-enriched domains, diffuse intracellular staining, or both. The percentage of transfected cells is expressed as the average of three experiments in which 150-190 cells were quantitated for each condition. Bars indicate the standard deviation.
[View Larger Version of this Image (36K GIF file)]



DISCUSSION

Protein phosphorylation of ligand-gated ion channels is a primary mode of regulation of their function and may play a central role in the regulation of synaptic transmission (15, 43). NMDA receptors are ligand-gated ion channels that are critical for several forms of activity-dependent synaptic plasticity in the brain such as long term potentiation and long term depression (3). Thus, the regulation of NMDA receptor function by protein phosphorylation could have dramatic effects on these processes. PKC has been suggested to regulate NMDA receptor function since intracellular perfusion of PKC in spinal trigeminal neurons potentiates NMDA receptor function (20). In addition, PKA has recently been suggested to potentiate NMDA receptors in hippocampal neurons in culture (25). Phorbol ester activation of PKC can also potentiate NMDA responses in Xenopus oocytes expressing homomeric receptors consisting of the NR1 subunit (13, 23) and heteromeric receptors consisting of the NR1 subunit and either the NR2A or NR2B subunit (8, 24). Interestingly, although homomeric NR1 receptors are potentiated by phorbol esters, receptors comprised of the NR1 and NR2C or 2D subunits are not affected by phorbol esters (8, 24). Moreover, NR1 splice variants which lack the C1 exon are dramatically potentiated by phorbol esters (13), in spite of the fact that most, if not all, of the PKC phosphorylation sites on NR1 appear to be contained within this exon (26). These results suggest that phorbol ester regulation of NMDA receptor function in Xenopus oocytes is complex and may be mediated in part by phosphorylation of endogenous proteins in the oocytes.

Previous results from our laboratory have demonstrated that PKC directly phosphorylates the NR1 subunit and that this phosphorylation can be eliminated by mutation of serines 889, 890, 896, and 897 in the C-terminal domain of NR1. Here we have further characterized the phosphorylation of the NR1 subunit using site-specific mutagenesis and phosphorylation site-specific antibodies. We have demonstrated that in addition to PKC, PKA directly phosphorylates the NR1 subunit of the NMDA receptor in vitro, in transfected cells, and in hippocampal slices. In addition, using site-specific mutagenesis and phosphorylation site-specific antibodies, we have shown that PKC phosphorylates serine 890, serine 896, and threonine 879, whereas PKA specifically phosphorylates serine 897 in vitro and in vivo. Moreover, activation of PKA and PKC results in the simultaneous phosphorylation of neighboring serine residues 896 and 897.

NMDA receptors in the brain cluster in the postsynaptic membrane and are also found in intracellular pools of unknown function (28, 44-46). Although the molecular mechanisms behind synaptic targeting are poorly understood, increasing evidence suggests that NMDA receptors may interact with the cytoskeleton (47). Recent studies have demonstrated that the NR1 subunit localizes to discrete receptor-rich domains in fibroblasts and that the formation of these receptor-rich domains is regulated by both alternative splicing and protein phosphorylation of the C1 exon (29). The C1 cassette has also recently been demonstrated to bind calmodulin (48). In this study we show that only phosphorylation of serine 890 by PKC, and not PKC phosphorylation of serine 896 or PKA phosphorylation of serine 897, affects NR1 distribution. One intriguing possibility is that PKC phosphorylation of serine 890 specifically regulates the interaction of the NR1 subunit with cytoskeletal proteins. It is interesting to note that the C1 cassette of the NR1 subunit is highly homologous to the effector domain of the cytoskeletal organizing protein MARCKS (myristoylated, alanine-rich, protein kinase C substrate), a protein initially identified as one of the major cellular substrates for PKC (49). The effector domain of MARCKS contains the major PKC phosphorylation sites as well as binding sites for actin and calmodulin. This region of the MARCKS protein is thought to mediate the reversible attachment of the actin cytoskeleton with the plasma membrane (49). In addition, the binding of the MARCKS effector domain to actin and calmodulin is inhibited by PKC phosphorylation. Thus, in a manner similar to the MARCKS protein, PKC phosphorylation of the C1 cassette of NR1 may play an important role in the regulation of the interaction of NR1 with the cytoskeleton. Future studies will address the role of the C1 cassette in regulating NR1 targeting in neurons and the role of PKC phosphorylation and calmodulin in regulating this process.

Essential to these studies was the generation of phosphorylation site-specific antibodies. These antibodies show remarkable specificity, with each antibody only recognizing NR1 when it was phosphorylated on a specific serine residues. These antibodies were useful for the study of NMDA receptor phosphorylation in vitro, in transfected cells, and in hippocampal slices. Moreover, these antibodies can differentiate between adjacent phosphoserine residues and are specific for the simultaneous phosphorylation of these two neighboring serine residues. These antibodies should be powerful reagents to study the regulation of NR1 phosphorylation in vivo during a variety of experimental paradigms such as model systems for the study of synaptic plasticity, activity-dependent neuronal development, and excitotoxicity.


FOOTNOTES

*   The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
   To whom correspondence should be sent: Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Dept. of Neuroscience, 725 North Wolfe St., 900 PCTB, Baltimore, MD 21205-2185. Tel.: 410-955-4050; Fax: 410-955-0877; E-mail: rick.huganir{at}qmail.bs.jhu.edu.
1    The abbreviations used are: NMDA, N-methyl-D-aspartate; PKC, protein kinase C; PKA, cAMP-dependent protein kinase; PMA, phorbol 12-myristate 13-acetate; PDA, phorbol 12,13-diacetate; FMOC, N-(9-fluorenyl)methoxycarbonyl; PBS, phosphate-buffered saline; PVDF, polyvinylidene difluoride; PAGE, polyacrylamide gel electrophoresis; MARCKS, myristoylated, alanine-rich, protein kinase C substrate.

Acknowledgments

We thank Dr. Sheenah Mische and Dr. Farzin-Gharahdaghi of the Rockefeller University for providing us with the initial protocol for phosphorylation of peptides. We thank Andy Mammen and Lit-Fui Lau for helpful comments on the manuscript.


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