Structural Determinants of Nicotinic Acid Adenine Dinucleotide Phosphate Important for Its Calcium-mobilizing Activity*

(Received for publication, April 15, 1997, and in revised form, June 3, 1997)

Hon Cheung Lee Dagger and Robert Aarhus

From the Department of Physiology, University of Minnesota, Minneapolis, Minnesota 55455

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENT
REFERENCES


ABSTRACT

Nicotinic acid adenine dinucleotide phosphate (NAADP) mobilizes Ca2+ through a mechanism totally independent of cyclic ADP-ribose or inositol trisphosphate. The structural determinants important for its Ca2+ release activity were investigated using a series of analogs. It is shown that changing the 3-carboxyl group of the nicotinic acid (NA) moiety in NAADP to either an uncharged carbinol or from the 3-position to the 4-position of the pyridine ring totally eliminates the Ca2+ release activity. Conversion of the 3-carboxyl to other negatively charged groups, either 3-sulfonate, 3-acetate, or 3-quinoline carboxylate, retains the Ca2+ release activity, although their half-maximal effective concentrations (EC50) are 100-200-fold higher. Changing the 6-amino group of the adenine to a hydroxyl group results in more than a 1000-fold decrease in the Ca2+ release activity. Conversion of the 2'-phosphate to 2',3'-cyclic phosphate or 3'-phosphate likewise increases the EC50 by about 5- and 20-fold, respectively. Similar to NAADP, all of the active analogs can also desensitize the Ca2+ release mechanism at subthreshold concentrations, suggesting that this novel property is intrinsic to the release mechanism. The series of analogs used was produced by using ADP-ribosyl cyclase to catalyze the exchange of the nicotinamide group of various analogs of NADP with various analogs of NA. An important determinant in NA that is crucial to the base exchange reaction was shown to be the 2-position of the pyridine ring. Neither pyridine-2-carboxylate nor 2-methyl-NA support the exchange reaction. The negative charge and the position of the 3-carboxyl group are nonessential since both pyridine-3-carbinol and pyridine-4-carboxylate support the base exchange reaction. In addition to the information on the structure-activity relationships of NAADP and NA, this study also demonstrates the utility of the base exchange reaction as a general approach for synthesizing NAADP analogs.


INTRODUCTION

A novel mechanism for mobilizing intracellular Ca2+ stores has recently been characterized in sea urchin eggs, which is activated by nicotinic acid adenine dinucleotide phosphate (NAADP),1 a metabolite of NADP (1, 2). The mechanism is independent of those activated by either inositol trisphosphate (IP3) or cyclic ADP-ribose (cADPR), since it is insensitive to inhibition by their respective antagonists, heparin and 8-amino-cADPR (2). The Ca2+ stores that are sensitive to NAADP can be separated by Percoll gradient density centrifugation from those sensitive to IP3 and cADPR (2), and these stores are also insensitive to thapsigargin, suggesting the presence of a novel Ca2+ pump (3). That the action of NAADP is likely to be mediated by a receptor is shown by specific binding of [32P]NAADP to egg microsomes, which can be competitively inhibited by NAADP but not by cADPR (4). Another unique feature that distinguishes this mechanism is that the agonist, NAADP, at subthreshold concentrations in the range of nanomolar, can totally inactivate the release mechanism (4, 5). This novel desensitization is shown to be occurring at the receptor level (4). A caged analog of NAADP has been synthesized (6), and releasing NAADP in live sea urchin eggs by photolyzing the analog produces not only a Ca2+ transient but long term Ca2+ oscillations lasting for more than 30 min (4), indicating that the Ca2+ release mechanism is present and operative in live cells.

NAADP, although structurally and functionally distinct from cADPR, can be synthesized enzymatically by ADP-ribosyl cyclase (1), the same enzyme that cyclizes NAD to produce cADPR (7). The cyclase can also use NADP as substrate and exchange the nicotinamide group of NADP with nicotinic acid (NA) at acidic pH to form NAADP (1). A soluble form of the cyclase has been purified and crystallized, and its crystal structure solved at 2.4 Å (8-10). The enzyme crystallizes as a dimer formed by two monomers in a head-to-head fashion, enveloping a central cavity (9). In this study, the structural determinants in NA critical for the base exchange reaction were assessed by testing a series of analogs of NA for their ability to support the reaction catalyzed by the cyclase. The products, analogs of NAADP, were assayed for Ca2+ release activity to determine the structure-function relationship of NAADP and its Ca2+-mobilizing activity.


EXPERIMENTAL PROCEDURES

Synthesis

NADP (1 mM) or its analog was incubated with NA or its analog (20-30 mM) at pH 4.5 with the Aplysia ADP-ribosyl cyclase (0.2 µg/ml) for 1-2 h at 20-23 °C, and the products were purified by HPLC using either an AG MP-1 column as described previously (1) or the column described below. All substrates were prepurified by the same HPLC method. An alternative HPLC procedure used a Poros 20 HQ column on a BioCad HPLC system (PerSeptive Biosystems) with a flow rate of 10 ml/min. A nonlinear gradient of trifluoroacetic acid was used: 0-1.33 min, 100% A (water); 1.33-1.83 min, linearly to 4% B (B was 150 mM trifluoroacetic acid); 1.83-2.66 min, linearly to 8% B; 2.66-3.49 min, linearly to 16% B; 3.49-5.15 min, linearly to 32% B; 5.15-6.81 min, linearly to 100% B; at 6.81 min stepped down to 0% B and maintained until 7.68 min.

Ca2+ Release in Sea Urchin Egg Homogenates

Homogenates of sea urchin egg (Strongylocentrotus purpuratus) were prepared as described previously (6). Frozen egg homogenates (25%) were thawed at 17 °C and sequentially diluted to 1.25% with a medium containing 250 mM N-methylglucamine, 250 mM potassium gluconate, 20 mM Hepes, 1 mM MgCl2, 2 units/ml creatine kinase, 8 mM phosphocreatine, 0.5 mM ATP, and 2 µM fluo 3 (Molecular Probes, Inc.), pH 7.2, adjusted with acetic acid (6). Ca2+ release was measured spectrofluorimetrically with an excitation wavelength of 485 nm and emission wavelength of 535 nm. The measurements were done in a cuvette maintained at 17 °C, and the homogenates were continuously stirred. The volume of homogenate used was 0.2 ml, and additions were usually made in 2-µl volumes.

Materials

ADP-ribosyl cyclase was purified from Aplysia ovotestis as described previously (7, 8). [14C]NADP was synthesized by phosphorylating [14C]NAD (Amersham Corp.) with 10 mM ATP using a NAD kinase partially purified from sea urchin egg extracts as described previously (4). The radioactive product, [14C]NADP, was purified by HPLC. The concentrations of various analogs of NAADP were determined by absorbance using extinction coefficients of 16,000 at 250 nm for deamino-NAADP, 48,000 at 260 nm for 3-QCA-ADP, and 18,000 at 260 nm for the rest of the analogs. The extinction coefficients were determined by the absorbance values and by quantifying the phosphate content of the analogs. Samples were treated with 45 units/ml alkaline phosphatase and 1.6 units/ml nucleotide pyrophosphatase for 1 h at 37 °C with 4.5 mM MgCl2, and the amounts of inorganic phosphate liberated were measured using a malachite green reagent as described previously (6, 11).

Analogs of NA, 3-QCA, 3-PAA, 3-PSA, 3-PC, and 2-methyl-NA were from Aldrich. P-4-COOH, deamino-NADP, 2':3'-cyclic NADP, and 3'-NADP were from Sigma. P-2-COOH was from Fluka.


RESULTS

ADP-ribosyl cyclase cyclizes NAD to produce cADPR (7, 12, 13). The enzyme can also use NADP as substrate efficiently. Fig. 1 shows the two alternative catalytic pathways of the cyclase using NADP as substrate. At neutral and alkaline pH, the enzyme cyclizes NADP by linking position N-1 of the adenine ring with the anomeric carbon of the terminal ribose to produce cADPR phosphate (cADPRP) (1). At acidic pH and in the presence of NA, the cyclase predominantly catalyzes the exchange of the nicotinamide group of NADP with NA and produces NAADP (1). The structural determinants of NA that are important for the base exchange reaction can be determined by assessing analogs of NA for support of the reaction, and some of them are listed in Fig. 1. The products of the base exchange reaction, analogs of NAADP, can then be used to analyze their structure-function relationships with NAADP and their Ca2+-releasing activity.


Fig. 1. Two catalytic pathways of ADP-ribosyl cyclase. The cyclase produces cADPR by cyclizing NAD. It can also cyclize NADP by linking the position N-1 of the adenine with the terminal ribose. In the presence of nucleophiles such as NA, the cyclase catalyzes the exchange of the nicotinamide group (Nic) of NADP with NA producing NAADP. By using various analogs of NA and NADP, a variety of analogs of NAADP can be synthesized, and some of those used in this study are listed. Modifications include those on the pyridine ring (R and R1), the 6-amino group of the adenine (R4), and the phosphate group at the 2'- (R2) or 3'-position (R3).
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Structural Determinants of Nicotinic Acid Important for the Base Exchange Reaction

Fig. 2 shows HPLC chromatograms of the products of the base exchange reaction using NADP and NA or one of its three analogs as substrate. At acidic pH with NA as substrate, the predominant product was NAADP (Fig. 2A). A small amount of cADPRP was also produced. Similar results were seen with P-4-COOH as substrate (Fig. 2B), which has a carboxyl group at the 4-position of the pyridine ring instead of the 3-position in NA. However, if the carboxyl group is at the 2-position (Fig. 2C, P-2-COOH), no base exchange product was observed; instead, the cyclase cyclized NADP to cADPRP. It appears that it is not just the position of the carboxyl group that is critical; in fact, any substitution at the 2-position may be sufficient to render it incapable of supporting the base exchange reaction. This is shown in Fig. 2D using an NA analog with an extra methyl group at the 2-position (2-methyl-NA). Again, no base exchange product was observed.


Fig. 2. HPLC chromatograms of the reaction products of the exchange reaction catalyzed by ADP-ribosyl cyclase. The cyclase (0.2 µg/ml) was incubated with 1 mM NADP at room temperature at pH 4.5 for 60 min and with 20 mM NA (A), P-4-COOH (B), P-2-COOH (C), or 2-methyl-NA (D). The base exchange products, NAADP and its analog, 4-NAADP, are present in panels A and B, but only the cyclization product cADPRP is seen in panels C and D. The HPLC analyses were done with a Poros 20 HQ column on a BioCad system.
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The time courses of the reaction with P-4-COOH or P-2-COOH as substrates are shown in Fig. 3. About 20% of the products was cADPRP with P-4-COOH and NADP as reactants, and the rest was the base exchange product, 4-NAADP (Fig. 3A). On the other hand, with P-2-COOH as substrate, the sum of cADPRP and the residual NADP was conserved throughout the reaction, indicating cADPRP was the only product (Fig. 3B). The base exchange product, 4-NAADP, an analog of NAADP with a carboxyl group at the 4-position instead of the 2-position of the pyridine ring, was tested for its Ca2+ release activity. At concentrations as high as 30 µM, it produced no Ca2+ release in sea urchin egg homogenates (Fig. 3A, inset), whereas NAADP elicited maximal Ca2+ release at 200-fold lower concentrations in the same preparation. Therefore, the position of the carboxyl at the pyridine ring is a critical determinant of the Ca2+ release activity, showing the exquisite specificity of the release mechanism.


Fig. 3. Time courses of the base exchange and cyclization reactions catalyzed by ADP-ribosyl cyclase. Experimental conditions were as described in Fig. 2. The inset in panel A shows that 4-NAADP, the base exchange product of P-4-COOH and NADP, was not active in releasing Ca2+ from sea urchin egg homogenates. B, the P-2-COOH analog of nicotinic acid did not support the base exchange reaction, and only the cyclization product of NADP, cADPRP, was produced.
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To determine if the charge of the carboxyl group is important for the base exchange reaction a neutral analog of NA, 3-PC, with a carbinol group replacing the carboxyl group was tested. The chromatogram of the products after incubation with the cyclase is shown in Fig. 4A. To follow the exchange of the nicotinamide group, [nicotinamide-14C]NADP was added as tracer. In addition to the 3-PC peak, the absorbance tracing of the products showed a peak with elution time the same as the starting substrate, NADP (Fig. 4B). This peak was the product of the exchange reaction, 3-PC-ADP, and not NADP itself since the peak was devoid of 14C radioactivity (Fig. 4A). All the radioactivity eluted with nicotinamide, which co-migrated with the large 3-PC peak. 3-PC was so effective in forcing the cyclase to the base exchange mode that very little cADPRP, the cyclization product, was detected in its presence (Fig. 4A). In the absence of the NA analog, the cyclase readily cyclized NADP to form cADPRP as shown in Fig. 4B.


Fig. 4. The base exchange reaction with 3-pyridyl carbinol as substrate. [nicotinamide-14C]NADP (1 mM, 60,000 cpm) was incubated with the cyclase (0.2 µg/ml) at pH 5 for 30 min with (A) or without (B) 20 mM 3-PC. Radioactivity (filled squares) in 10% of each fraction was determined. The peak labeled 3-PC-ADP in panel A has the same elution time as NADP shown in panel B, and it was the product of the base exchange reaction since it lost the 14C label. HPLC was done with an AG MP-1 column.
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The effectiveness of the uncharged analog, 3-PC, in supporting the exchange reaction raises the possibility that the uncharged form of NA may be the reactive species. This could explain the acidic requirement for the base exchange reaction (1). NA has a pK value of about 4.8 and about half would be uncharged at the pH of the reaction (pH 4.5). The neutral reactant requirement was tested using an analog of NA, 3-PSA, with a sulfonic acid group at the 3-position instead. The sulfonate is expected to be fully charged at the pH of the reaction. Fig. 5 shows that 3-PSA fully supported the base exchange reaction. After 40 min of reaction, about equal amounts of the exchange product, 3-PSA-ADP, and cADPRP were produced. The proportion of cADPRP was higher with 3-PSA as substrate than when NA was used (cf. Fig. 2), which could be due to the difference in size of the sulfonate group as compared with the carboxyl group. These results show that either a neutral or a fully charged group at the 3-position of the pyridine ring is capable of supporting the exchange reaction.


Fig. 5. The base exchange reaction with 3-pyridylsulfonic acid as substrate. NADP (1 mM) was incubated with 20 mM 3-PSA at pH 5 before (T = 0 min) or after 40 min with the cyclase (0.2 µg/ml) (T = 40 min). HPLC was done with an AG MP-1 column. The peak corresponding to the product of the base exchange reaction is labeled 3-PSA-ADP.
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Structure-Function Relationship of NAADP and Its Ca2+ Release Activity

Unlike the analog 4-NAADP described above, 3-PSA-ADP elicited full Ca2+ release from egg homogenates as shown in Fig. 6. It also desensitized the homogenates to subsequent challenge with NAADP. Likewise, homogenates desensitized to NAADP would not respond to 3-PSA-ADP. A unique property of the NAADP-sensitive Ca2+ release mechanism is that NAADP can induce complete self-inactivation at subthreshold concentrations (4, 5). Treatment of egg homogenates with non-releasing concentrations of 3-PSA-ADP for 3 min produced such inactivation that saturating NAADP added afterward induced no further Ca2+ release (Fig. 6). Similarly, homogenates inactivated by pretreatment with nanomolar concentrations of NAADP did not respond to maximal concentrations of 3-PSA-ADP. These results indicate that 3-PSA-ADP acts on the same Ca2+ release site as NAADP.


Fig. 6. The Ca2+ release activity of an analog of NAADP. The base exchange product, 3-PSA-ADP, produced using 3-pyridylsulfonic acid as substrate (described in Fig. 5) was tested for its Ca2+ release activity in sea urchin egg homogenates using fluo 3 as a Ca2+ indicator. The last two sets of tracings show that at non-releasing concentrations either 3-PSA-ADP (0.5-2 µM) or NAADP (0.5-2 nM) can inactivate the Ca2+ release mechanism such that a subsequent challenge with the other agonist is much less effective.
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Fig. 7 compares the concentration response of four analogs of NAADP with different substitutions on the pyridine ring. At the 3-position, these substitutions are sulfonic acid for 3-PSA-ADP, acetic acid for 3-PAA-ADP, and carbinol for 3-PC-ADP. Also shown is the analog with 3-quinoline carboxylic acid substituting for NA, 3-QCA-ADP. The half-maximal effective concentration (EC50) of 3-PSA-ADP was about 3 µM, more than 200-fold higher than that of NAADP. The EC50 values for 3-PAA-ADP were even higher at about 10 µM. For 3-QCA-ADP, the largest in size of the series, only a small Ca2+ release was detected at 10 µM. It thus appears that the effectiveness of this series of analogs in releasing Ca2+ decreases roughly with increasing size of the substitution on the pyridine ring. However, the negative charge of the substitution is definitely critical. In the absence of a negative charge, as in the case of 3-PC-ADP, an analog with a carbinol group at the 3-position and the smallest in size of the series, there was no Ca2+ release activity even at 10 µM. This charge requirement is consistent with the previous demonstration that NADP, with an uncharged amide group at the 3-position of the pyridine ring, is likewise inactive in releasing Ca2+ (2, 14).


Fig. 7. Concentration-response curves of the Ca2+ release activity of NAADP analogs. A series of analogs with various modifications at the 3-position of the pyridine ring was synthesized as described in the text. The conditions for the Ca2+ release assay were as described in Fig. 6.
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In addition to the analogs with various substitutions in the pyridine ring described above, structural changes can also be imposed on other parts of the NAADP molecule by using analogs of NADP as substrates for the exchange reaction with NA. Results shown in Fig. 8 indicate that the position of the phosphate group at the 2'-position of the ribose is important but not critical for the Ca2+ release activity. Changing the 2'-phosphate to a cyclic form linking both the 2'- and 3'-positions, 2',3'-cyclic NAADP, decreases its effectiveness but does not eliminate its Ca2+ release activity. A further decrease in effectiveness is seen when the 2'-phosphate is changed to the 3'-position as in 3'-NAADP in which EC50 increases by about 20-fold to 0.3 µM as compared with 15 nM for NAADP. Therefore, as long as a phosphate group is present at the ribose, the Ca2+ release activity is retained. Removal of the phosphate as in NAAD, however, does eliminate its biological activity as has been shown previously (2).


Fig. 8. Changes in the Ca2+ release activity of NAADP analogs with modifications at the 2'-phosphate. The analogs were synthesized using 2',3'-cNADP or 3'-NADP and NA as substrates for the base exchange reaction. The Ca2+ release activity of the corresponding products, 2',3'-cNAADP and 3'-NAADP, was assayed and compared with that of NAADP using conditions as described in Fig. 6.
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An unexpected structural determinant in NAADP that is critical for its Ca2+ release activity is the amino group at the 6-position of the adenine ring. Using nicotinamide hypoxanthine dinucleotide phosphate (deamino-NADP) and NA as substrates, deamino-NAADP was synthesized. Fig. 9 shows that the Ca2+ release activity of the analog was only detectable at higher than 5 µM. It did, however, inactivate the release mechanism at non-activating concentrations in a manner similar to NAADP, indicating that it did act on the NAADP-sensitive mechanism. This is in contrast to 3-PC-ADP that did not appear to interact with the NAADP receptor at all since it neither released Ca2+ nor induced desensitization.


Fig. 9. Ca2+ release activity and desensitization induced by analogs of NAADP. Deamino-NAADP was synthesized using deamino-NADP and NA as substrates for the base exchange reaction. The carbinol derivative of NAADP, 3-PC-ADP, was synthesized as described in Fig. 4. The Ca2+ release activity was assayed as described in Fig. 6. For the desensitization experiments, egg homogenates were first treated for 5 min with the indicated concentration of the analog and subsequently challenged with 0.14 µM NAADP.
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DISCUSSION

The Ca2+-releasing activity of NAADP is novel in three aspects. First, the release mechanism activated by NAADP is completely independent of the two known mechanisms mediated by the IP3 and ryanodine receptors, indicating that it is a new and hitherto undescribed pathway (1-5). Second, the NAADP-activated mechanism is the first receptor-mediated mechanism that shows full inactivation by non-stimulating concentrations of the agonist (4, 5). Third, releasing NAADP in live sea urchin eggs by photolyzing its caged analog produces long term Ca2+ oscillations, a function shared by neither IP3 nor cADPR (4). These three unique and novel features make the NAADP-dependent Ca2+-signaling mechanism a worthy topic of investigation. This study represents the first to systematically analyze the structure-activity relationship between NAADP and its Ca2+-releasing function.

It is shown that there are at least three critical structural determinants on NAADP. The first one is the negative charge at the 3-position of the pyridine ring. Elimination of the charge or changing it to the 4-position completely inactivates the molecule. The site has some tolerance toward the size of the group containing the negative charge, and the analogs show a decrease in effectiveness as the bulkiness of the group increases. The second important determinant is the 2'-phosphate. The requirement is lenient as long as a phosphate group is attached to the ribose. The molecule retains activity whether the phosphate is on the 2'- or 3'-position or cyclically linked to both positions. Removal of the phosphate as in NAAD, however, eliminates its Ca2+ release activity (2). The third important site is the amino group at the 6-position of the adenine ring. Conversion to an -OH group reduces the effectiveness of its Ca2+ release activity by more than 1000-fold, even though -OH is just about the same size as -NH2. These results indicate that the NAADP-sensitive Ca2+ release mechanism is highly specific and are consistent with it being mediated by a high affinity receptor (4).

This study also provides information on the unique self-inactivation property of the NAADP receptor. All of the analogs tested that are agonists can also induce inactivation at subthreshold concentrations. The efficacy of the analogs in inducing inactivation directly parallels the efficacy in releasing Ca2+. Analogs that have no Ca2+-releasing activity also do not cause inactivation. This inextricable linkage between activation and inactivation suggests the inactivation is likely to be intrinsic to the function of the receptor. It is possible that the NAADP receptor contains two binding sites for NAADP. The regulatory site would be of high affinity, and its occupancy results in inactivation. The other site would be of lower affinity and represents the Ca2+-releasing site. This two-site mechanism is consistent with the present results.

The series of analogs used in this study was synthesized by taking advantage of the base exchange reaction catalyzed by the Aplysia ADP-ribosyl cyclase (1, 7, 8). By choosing systematically a set of NA analogs it can be concluded that one of the most critical determinants for the base exchange reaction is the 2-position of the pyridine ring. Any substitution at that position makes the analog incapable of supporting the reaction. It has previously been proposed that the base exchange reaction results from an attack by nicotinic acid on the anomeric carbon of an activated ADP-ribose moiety that is formed as an enzyme intermediate during catalysis by the cyclase (15, 16). Substitution at the 2-position apparently reduces the reactivity of the nitrogen of the pyridine ring either by steric hindrance or by reduction of its nucleophilicity. As a result, the chance for the competing intramolecular attack by the N-1 of the adenine of the activated ADP-ribose increases, resulting in the cyclization reaction being the predominant pathway.

The carboxyl group and its negative charge are nonessential for the base exchange reaction. Indeed, the neutral 3-PC was so effective in promoting the base exchange reaction that the cyclization reaction appeared inhibited by its presence. The analog, however, did not really inhibit the cyclase but instead forced the cyclase into the exchange pathway. The apparent inhibition is due to the fact that the product of the exchange has the same HPLC elution time as the substrate NADP, making it appear that no product was formed. Nicotinamide behaves in much the same manner as 3-PC (data not shown). In the absence of nicotinamide, the cyclase readily cyclizes NAD or NADP. The presence of nicotinamide forces the enzyme into the exchange mode, and since the product and the substrate are identical, the enzyme appears inhibited. These results caution against the casual use of nicotinamide and similar compounds as inhibitors of the cyclase without careful determination if the enzyme is forced into an exchange mode instead.

This study demonstrates the usefulness of the base exchange reaction for synthesizing a wide variety of analogs of NAADP. Although only a small number of commercially available substrate analogs was used in this study, the demonstrated feasibility of the approach should set the stage for more thorough investigations into other structural determinants on NAADP or NA that are important for the Ca2+-releasing and enzymatic activities, respectively. The large number of pyridine derivatives and analogs of NADP available make this simple synthesis particularly versatile. In addition to the information on the structure-activity relationships of NAADP and NA, the approach can conceivably lead also to the synthesis of other novel analogs of NAADP, such as fluorescent analogs that may be useful in visualizing the NAADP-sensitive Ca2+ stores in cells.


FOOTNOTES

*   This work was supported by National Institutes of Health Grant HD17484 (to H. C. L.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Dagger    To whom correspondence should be addressed: 6-182 Lyon Laboratory, Dept. of Physiology, University of Minnesota, Minneapolis, MN 55455. Tel.: 612-625-7120; Fax: 612-625-0991; E-mail: leehc{at}maroon.tc.umn.edu; http://enlil.med.umn.edu/www/phsl/faculty/hcl1.htm.
1   The abbreviations used are: NAADP, nicotinic acid adenine dinucleotide phosphate; NAAD, nicotinic acid adenine dinucleotide; cADPR, cyclic ADP-ribose; cADPRP, cADPR phosphate; IP3, inositol 1,4,5-trisphosphate; NA, nicotinic acid; 3-PSA, 3-pyridinesulfonic acid; 3-PSA-ADP, 3-PSA adenine dinucleotide phosphate; 3-PAA, 3-pyridylacetic acid; 3-PAA-ADP, 3-PAA adenine dinucleotide phosphate; 3-QCA, 3-quinoline carboxylic acid; 3-QCA-ADP, 3-QCA adenine dinucleotide phosphate; 3-PC, 3-pyridylcarbinol; 3-PC-ADP, 3-PC adenine dinucleotide phosphate; 2-methyl-NA, 2-methylnicotinic acid; P-4-COOH, pyridine-4-carboxylic acid; 4-NAADP, pyridine-4-carboxylic acid NAADP; P-2-COOH, pyridine-2-carboxylic acid; HPLC, high pressure liquid chromatography.

ACKNOWLEDGEMENT

We thank Richard Graeff for critical reading of the manuscript.


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