(Received for publication, March 28, 1996, and in revised form, November 15, 1996)
From the Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Yoshida, Sakyo-ku, 606 Kyoto, Japan
To identify cell death-induced genes, we employed
a subtractive hybridization approach and isolated a cDNA encoding a
mouse homolog of carnitine palmitoyltransferase I (CPT I), an enzyme that resides at the outer mitochondrial membrane and facilitates passage of long-chain fatty acids into mitochondria for -oxidation. Induced expression of CPT I mRNA was observed upon programmed cell
death in the murine hematopoietic cell lines LyD9 and WEHI-231. To
elucidate the role of CPT I in programmed cell death, we examined the
effects of long-chain fatty acids and found that the addition of
palmitate or stearate to cultured cells led to activation of a death
program with a morphology resembling that of apoptosis. Other naturally
occurring fatty acids, including myristate and palmitoleate, had no
effect. Since both palmitate and stearate are sphingolipid precursors,
the effect of these fatty acids on sphingolipid metabolism was tested.
Our results indicate that apoptosis induced by palmitate or stearate is
correlated with de novo synthesis of ceramide. Inhibition
of CPT I by etomoxir enhanced palmitate-induced cell death and led to a
further increase in ceramide synthesis.
Superfluous cells frequently arise during development of multicellular organisms (1-4). In addition, cells damaged by mutational events may emerge anytime in life and lead to cancer. Efficient removal of these cells is important to maintain morphogenesis and homeostasis and is achieved by an intrinsic mechanism resulting in cellular suicide. This death appears to be genetically controlled and has been termed programmed cell death. Tight regulation is essential since both excessive cell death and insufficient cell death will disrupt tissue integrity and may have fatal consequences for the organism. Cells undergoing the death program can often be recognized by a number of distinct morphological changes that have been described as apoptosis (5, 6).
Several genes regulating programmed cell death have been identified, and some insight into their function has been obtained (7). Induced expression of a short polypeptide encoded by the reaper gene, for example, precedes apoptosis in Drosophila melanogaster. Inactivation of reaper suppresses virtually all programmed cell death in Drosophila (8). The reaper polypeptide appears to have homology to the death domains found in the cytoplasmic region of several mammalian cell-surface receptors, including CD95 and TNFR1 (9). Members of the interleukin 1-converting enzyme family of cysteine proteases also play an important role in the regulation of cell death. These enzymes promote apoptosis by converting inactive precursor polypeptides into their active form (10). The BCL-2 protein, on the other hand, often extends cell survival by inhibiting apoptosis (11, 12). Several BCL-2 homologs have been isolated, but their function remains vague.
Analysis of signal transduction pathways has pointed toward
sphingolipid metabolites as intracellular mediators of programmed cell
death. Ceramide, in particular, has been shown to incite antiproliferative responses and apoptosis (13, 14). Several stimuli
such as tumor necrosis factor-,
-interferon, and ionizing radiation initiate sphingomyelin hydrolysis, thereby generating ceramide (15-17). Downstream targets of ceramide action may include the retinoblastoma gene product c-myc and some protein
kinases (18, 19). Palmitoyl-CoA, an important precursor of
sphingolipids synthesized de novo, is also a substrate for
carnitine palmitoyltransferase I (CPT I),1
which resides at the outer mitochondrial membrane, and, together with
CPT II and a carnitine/acylcarnitine translocase, facilitates passage
of long-chain fatty acids into mitochondria for
-oxidation. CPT I
catalyzes the rate-limiting step in this process and is a site for
intracellular regulation of fatty acid metabolism, with malonyl-CoA
acting as a physiological inhibitor (20).
In this report, we demonstrate that two saturated fatty acids, palmitate and stearate, induce de novo synthesis of ceramide and programmed cell death. We show that inhibition of CPT I activity enhances both sphingolipid synthesis and palmitate-induced cell death.
bcl-2 cDNA was a kind gift from
Dr. D. Loh. Triacsin C and ISP1 were kindly provided by Dr. S. Omura
(Kitasato Institute, Tokyo, Japan) and Dr. T. Kawasaki (Kyoto
University, Kyoto, Japan), respectively. Etomoxir
(2-[6-(4-chlorophenoxy)hexyl]oxirane-2-carboxylic acid) was purchased
from ASAT AG (Zug, Switzerland). Fumonisin B1 and
C2-ceramide were from BIOMOL Research Labs Inc. (Plymouth Meeting, PA). L-[3-14C]Serine and the
sn-1,2-diacylglycerol assay system were from Amersham Corp.
Other chemicals were from Sigma and Wako Pure Chemical Industries (Osaka, Japan).
The murine cell lines LyD9 (21) and WEHI-231
(22) were cultured in RPMI 1640 medium (Life Technologies, Inc.) as
described (23). Long-chain fatty acids were dissolved in 30 mM KOH and gently heated when required. Fresh preparations
were used for induction of cell death. Triacsin C and
C2-ceramide were dissolved in ethanol and stored at
20 °C at concentrations of 2 and 40 mM, respectively.
ISP1 was in methanol (100 µM). The final concentration of
alcohol in cell cultures was
0.1%. Fumonisin B1 and
etomoxir were dissolved in water (10 mM each). Reagents
dissolved in water were prepared immediately before use as cell culture
additives.
Total cellular RNA was extracted by centrifugation through a cesium chloride cushion in the presence of guanidium isocyanate (24). Poly(A)+ RNA was purified by oligo(dT) column chromatography (24). The subtractive hybridization procedure was carried out as described (23), except that first-strand cDNA for library construction was generated using poly(A)+ RNA extracted from LyD9 cells that had been deprived of IL-3 for 6 h. For subtracted library construction, the Cr0t value (normalized for the presence of high salt) (25) was driven to 6565 mol/s/liter. The Cr0t value of a subtracted cDNA probe used for library screening reached 8307 mol/s/liter. This probe was radiolabeled to a specific activity of 2.5 × 109 dpm/µg. For plaque hybridizations, a sodium dodecyl sulfate-based hybridization solution (26) was used to improve the signal-to-noise ratio. Positive clones were picked; inserts were subcloned into a Bluescript® KS plasmid vector (Stratagene); and their nucleotide sequence was determined.
Cloning, Northern Analysis, and Sequencing of Mouse Liver CPT IFull-length cDNA was isolated from an ordinary LyD9 cDNA library, generated by standard procedures (24). Northern hybridizations were carried out as described (24). Nucleotide sequences were determined using a Model 373 DNA Sequencer® (Applied Biosystems, Inc.).
Detection of Programmed Cell DeathDNA ladder formation was detected as described previously (23). Nuclear fragmentation was analyzed on a FACScan® flow cytometer (Becton Dickinson) employing the method of Nicoletti et al. (27). Briefly, cells were collected and washed twice in cold phosphate-buffered saline. Samples were lysed and stained with propidium iodide for 1 h at room temperature before fluorescence-activated cell sorting analysis.
Thin-layer ChromatographyFor the analysis of de novo synthesized sphingolipids, LyD9 cells or WEHI-231 cells (5 × 106) were metabolically labeled for 3 h with [14C]serine (0.5 µM/ml) after starving them for 15 min in serine-free medium containing 10% dialyzed fetal calf serum. Lipids were extracted as described by Bligh and Dyer (28) and separated by one-dimensional TLC (29). Lipid extracts were dissolved in a volume of 20 µl of chloroform/methanol (2:1, v/v); 1-µl aliquots were spotted on TLC plates (Merck No. 5547) and separated in chloroform, methanol, 2 N ammonia (100:25:2.5, v/v/v). Radioactivity was detected and quantitated on a BAS 2000® image analyzer (Fujifilm).
Diacylglycerol Kinase AssayLipid extractions and ceramide
measurements were carried out as described (30), with the following
modifications. Lipids were dissolved in 20 µl of detergent consisting
of 7.5% (w/v) n-octyl -glucopyranoside and 5 mM cardiolipin in 1 mM DTPA. After sonication,
70 µl of a reaction mixture (containing 70 mM imidazole HCl, pH 6.6, 70 mM NaCl, 18 mM
MgCl2, 1.4 mM EGTA, 2.8 mM
dithiothreitol, and an Escherichia coli membrane preparation
enriched in DAG kinase) was added. Included standards were ceramide and
DAG. After TLC, radioactivity incorporated into ceramide phosphate and
phosphatidic acid was measured as described above.
The murine IL-3-dependent hematopoietic
precursor cell line LyD9 undergoes apoptosis after IL-3 deprivation
(21, 23). Apoptosis in LyD9 cells is inhibited by actinomycin D and
therefore requires de novo RNA synthesis. To isolate cell
death-associated genes, we screened a subtracted LyD9 cDNA library
enriched for cDNAs induced after IL-3 withdrawal. Differential
screening led to the isolation of a clone whose sequence is highly
homologous to that of rat liver CPT I (GenBankTM accession
number D49472[GenBank]) (31). Northern analysis confirmed the induced expression
of murine CPT I mRNA in IL-3-deprived LyD9 cells. To test whether
CPT I mRNA levels after death induction are also increased in other
cell lines, we used WEHI-231 cells, an immature B-cell line (22), and
found that CPT I gene expression was augmented after apoptosis, induced
by cross-linking of surface IgM (Fig. 1).
Induction of Apoptosis by Palmitate and Stearate
The enhanced
expression of CPT I suggested an involvement of fatty acid metabolism
in programmed cell death. We intended to test whether substrates of CPT
I, like palmitoyl-CoA, affect apoptosis. Because palmitoyl-CoA is not
cell-permeable, we tested the effect of palmitate, the direct
precursor of palmitoyl-CoA, on cell survival. As shown in Fig.
2A, palmitate induced cell death in LyD9
cells as effectively as C2-ceramide, a cell-permeable
ceramide analog previously shown to cause apoptosis (13). After the
addition of palmitate, mitotic activity ceased. The following cell
death was accompanied by cellular shrinkage, nuclear condensation, and nuclear fragmentation, a morphology typically observed in cells undergoing apoptosis. Another long-chain fatty acid, stearate, had
similar effects. Other saturated fatty acids with carbon chain lengths
ranging from C4 to C14 were tested; however,
none of them could induce apoptosis. Furthermore, unsaturated fatty
acids with carbon chain lengths ranging from C16 to
C20, including several branched fatty acids, had no effect
on cell viability. Representative data from experiments using laurate,
myristate, palmitate, palmitoleate, and stearate, which have a similar
chemical structure, are shown in Fig. 2A. Cell death
induction by palmitate was also observed in WEHI-231 cells (Fig.
2B), but not in bcl-2-transfected WEHI-231 cells
(data not shown). As expected, induced expression of CPT I mRNA was
found also when cell death was triggered by palmitate (Fig. 1).
Impact of Lipid Metabolism Inhibitors on Apoptosis Induced by Palmitate
To determine whether CPT I activity can modulate
palmitate-induced cell death, we blocked the enzyme using etomoxir, a
highly specific irreversible CPT I inhibitor (32, 33). The drug was added to LyD9 cell cultures at concentrations that did not compromise viability (Fig. 3A). When etomoxir was
applied together with palmitate, a dramatic increase in DNA ladder
formation (Fig. 2B) and nuclear fragmentation (Fig.
3B) was observed. Indeed, concentrations of palmitate that
were not effective alone caused substantial cell death in combination
with etomoxir, as judged by the incidence of nuclear fragmentation
events (Fig. 3B). Similar results were obtained using
another cell line, WEHI-231 (data not shown). Thus, inhibition of CPT I
by etomoxir appears to reduce the threshold for palmitate-induced
apoptosis.
Long-chain fatty acids are not available as CPT I substrates until they are activated by acyl-CoA synthetase. The active form of etomoxir is its CoA ester, which is also formed by this enzyme (32). Therefore, both palmitate and etomoxir depend on acyl-CoA synthetase activity to gain access to the mitochondrial CPT system. Acyl-CoA synthetase activity is inhibited by a number of competitive inhibitors, with triacsin C being the most potent member of this group (34, 35). The addition of nontoxic concentrations of triacsin C to LyD9 cells grown in the presence of palmitate and etomoxir significantly reduced the occurrence of nuclear fragmentation (Fig. 3C). Thus, palmitoyl-CoA and/or etomoxir-CoA are necessary for apoptosis induction.
We speculated that inhibition of CPT I by etomoxir might prevent the entrance of palmitoyl-CoA into mitochondria and lead to its accumulation in the cytoplasm. Since palmitoyl-CoA is a precursor of sphingolipid synthesis, we considered the possibility that inhibition of CPT I leads to augmented production of ceramide. The initial step in ceramide synthesis is the formation of 3-ketodihydrosphingosine from palmitoyl-CoA and L-serine. This reaction is catalyzed by serine palmitoyltransferase, which is inhibited by the sphingosine analog ISP1 at picomole concentrations (36). The addition of ISP1 to LyD9 cells cultured with palmitate and etomoxir markedly reduced nuclear fragmentation (Fig. 3C). Similar data were obtained using fumonisin B1, which suppresses ceramide synthetase activity (37), the final step in de novo synthesis of ceramide. These results suggest that de novo synthesized sphingolipids may be mediators of apoptosis induced by simultaneous treatment with palmitate and etomoxir.
De Novo Synthesis of Ceramide and Sphingomyelin upon Palmitate-induced ApoptosisTo verify that the addition of
palmitate causes de novo synthesis of ceramide, LyD9 cells
were metabolically labeled with [14C]serine. After lipid
extraction, samples were analyzed by TLC. The amount of de
novo synthesized sphingolipids was compared among samples treated
with various combinations of the inhibitors described above. The
results shown in Fig. 4 confirmed that palmitate
addition to cultured cells leads to de novo synthesis of
ceramide. An ~3-fold increase in radiolabeled ceramide was observed
in cells treated with 100 µM palmitate within 3 h as
compared with cells treated with carrier alone. The additional use of
etomoxir led to a further rise by a factor of 2. The use of palmitate
and etomoxir in combination with either ISP1 or fumonisin
B1 caused a reduction in de novo synthesized
ceramide. ISP1 blocked ceramide synthesis almost completely, whereas
fumonisin B1 was less effective, but still reduced ceramide build-up to a level similar to that seen in untreated cells (Fig. 4).
Of note, the amounts of ceramide synthesized in the presence of the
various reagents generally correlate with the extent of cell death
observed (Fig. 3).
To further validate the notion that palmitate-induced apoptosis is
mediated by de novo synthesized sphingolipids and that CPT I
confers a protective effect on this kind of cell death, it was
important to establish that cell membrane-derived sphingomyelin does
not contribute to ceramide production after treatment of cells with
palmitate. Total cellular ceramide content was determined in an assay
using E. coli DAG kinase. This assay allows the simultaneous measurement of ceramide phosphate derived from ceramide and of phosphatidic acid derived from DAG. The results substantiate our previous data that palmitate treatment leads to ceramide production that is enhanced in the presence of etomoxir (Fig.
5A). DAG levels were also increased in the
presence of palmitate and etomoxir (Fig. 5B). More
important, the use of ISP1 in addition to palmitate and etomoxir caused
a decrease in ceramide phosphate to levels well below those seen in
cells treated with carrier alone, indicating that, in fact, all
ceramide generated under these conditions is derived from de
novo synthesis. Therefore, sphingomyelin residing in the cell
membrane compartment does not appear to be released.
Different Effects of CPT I Inhibition on Sphingolipid Synthesis after Challenge with Palmitate and Stearate
Palmitoyl-CoA and stearyl-CoA are important components of ceramide and other sphingolipids. To test whether both fatty acids have similar effects on de novo sphingolipid synthesis, stearate-treated LyD9 cells were metabolically labeled with [14C]serine as described above. The ceramide increase detected after apoptosis induction by stearate was almost indistinguishable from that seen after palmitate treatment (Fig. 4). However, in contrast to the observations made after cell death induction with palmitate, inhibition of CPT I by etomoxir did not lead to an additional increase in ceramide levels when stearate was used to induce apoptosis (Fig. 4). In agreement with these findings, etomoxir could not accelerate stearate-induced cell death as efficiently as it did when palmitate was used (Table I).
|
In this report, we describe an experimental system that allows the investigator to study programmed cell death mediated by de novo synthesis of sphingolipids. Ceramide production is induced by culturing cells of hematopoietic origin in the presence of palmitate and is further enhanced by simultaneous administration of the CPT I inhibitor etomoxir. The use of the pharmacological inhibitors fumonisin B1 and ISP1, which interfere with ceramide synthesis, rescues cells from apoptosis (Fig. 3). Increased de novo synthesis of ceramide may ultimately lead to increased levels of other sphingolipids, such as sphingosine and sphingomyelin. The data presented here do not exclude the possibility that sphingolipids other than de novo synthesized ceramide are directly involved in the cell deaths observed. Increased levels of sphingomyelin, for example, may be a target for sphingomyelinase and in turn be degraded to ceramide again.
Sphingolipid metabolism has been shown to play an important role in the regulation of programmed cell death (38). We show that CPT I, a component of the mitochondrial CPT enzyme complex, can protect from palmitate-triggered cell death by down-regulation of sphingolipid synthesis. This conclusion is based mainly on three observations. First, cell death induced by palmitic acid was accelerated after inhibition of CPT I by etomoxir (Fig. 3 and Table I). Second, an ~2-fold increase in de novo synthesized ceramide was observed in cells treated with etomoxir and palmitic acid as compared with cells treated with palmitic acid alone (Fig. 4). The palmitate-mediated build-up of ceramide is blocked in the presence of ISP1 and, to a lesser extent, fumonisin B1. As a result, total cellular ceramide levels drop (Fig. 5). Cell membrane-derived sphingomyelin does not appear to be utilized to replenish ceramide levels because, in the presence of ISP1, ceramide levels drop below those seen in cells treated with carrier alone. The various aspects of sphingolipid metabolism are restricted to different cellular compartments (39). Sphingomyelin within the cell membrane may not be released without the appropriate signals. Third, the sphingolipids produced after simultaneous treatment with palmitate and etomoxir were the cause of apoptosis because inhibition of sphingolipid synthesis by ISP1 and fumonisin B1 blocked the death program (Fig. 3). It is important to stress that the amounts of ceramide produced in the presence of the various inhibitors correlate well with the extent of cell death observed (Figs. 3 and 4 and Table I). ISP1-dependent depletion of intracellular sphingolipid levels has recently been shown to cause cell death in the interleukin-2-dependent cytotoxic T-cell line CTLL-2 (40). As suggested by Nakamura et al. (40), this may be due to a cell lineage-specific sensitivity toward sphingolipid depletion since viability in several other cell lines tested by the authors, including another interleukin-2-dependent cell line (F7), was not compromised. In our hands, neither LyD9 nor WEHI-231 cell growth was affected by 100 nM ISP1.
The protection from apoptosis afforded by CPT I after death induction
with palmitate is likely to be the consequence of long-chain fatty
acyl-CoA clearance from the cytoplasm. The CPT enzyme complex effectively transfers activated long-chain fatty acids into the mitochondrial matrix, where they serve as fuel for -oxidation. Sphingolipid precursors, like palmitoyl-CoA, are subject to removal from the cytoplasm by this mechanism. Cells that express high amounts
of CPT I per se are therefore expected to withstand
palmitate-induced death. Indeed, hepatoma cell lines that are known to
be rich in mitochondria were resistant to palmitate-induced apoptosis
(data not shown).
In addition to palmitate, only stearate was able to induce apoptosis and led to increased ceramide synthesis. However, inhibition of CPT I by etomoxir did not enhance sphingolipid synthesis when stearate was used for cell death induction. Since palmitoyl-CoA and stearyl-CoA are important components of ceramide and both are substrates for CPT I, the reason for this difference was not immediately obvious. However, the two activated fatty acids enter the sphingolipid synthesis pathway at different points. While palmitoyl-CoA, the substrate for serine palmitoyltransferase, constitutes the major non-amide-linked fatty acid component of ceramide, stearate is the predominant amide-linked fatty acid component of ceramide (41). The addition of the stearyl group to the ceramide backbone is one of the last steps in ceramide synthesis, whereas the condensation of serine and palmitoyl-CoA is the first dedicated step in ceramide synthesis. It may be that an accumulation of palmitoyl-CoA as a result of CPT I inhibition immediately influences ceramide metabolism, while the accumulation of stearyl-CoA without sufficient palmitoyl-CoA resources cannot effectively boost ceramide synthesis. Therefore, the available amount of palmitoyl-CoA may be rate-limiting in de novo synthesis of ceramide. Moreover, stearyl-CoA may be replaced by other long-chain fatty acids like palmitoyl-CoA to form the amide-linked component of ceramide, whereas the high substrate specificity of serine palmitoyltransferase prevents the use of another long-chain fatty acyl-CoA instead of palmitoyl-CoA.
Inhibition of CPT I by etomoxir boosts cell death also in WEHI-231 cells (Fig. 2B), which have been shown to accumulate ceramide after cross-linking of surface Ig (42). Ceramide has been reported to incite a stress-activated protein kinase. Of note, a <2-fold increase in intracellular ceramide levels was sufficient to induce stress-activated protein kinase and apoptosis (19). Other protein kinases may also be activated by increased ceramide levels (43, 44).
CPT I association with programmed cell death was initially discovered based on enhanced expression of its mRNA in LyD9 cells deprived of IL-3 (Fig. 1). However, apoptosis in LyD9 cells induced by growth factor withdrawal was only slightly accelerated in the presence of etomoxir (data not shown). Moreover, de novo synthesis of ceramide after IL-3 deprivation was only marginal (Fig. 4). Therefore, the mitochondrial CPT system may play an additional, yet to be elucidated role in the lipid metabolism of programmed cell death. CPT I isoforms with overlapping tissue-specific expression have been characterized (45). CPT I isoform switching has recently been shown to take place in development (46). Proteins with CPT activity are also associated with peroxisomes and microsomes (47). Murthy and Pande (48) have described a microsomal stress-regulated protein containing CPT activity. Additional analysis of the mitochondrial CPT system in the context of the cellular stress response will be required to further define the apoptosis-related aspects of this multienzyme complex.