Modulation of E2F Activity Is Linked to Interferon-induced Growth Suppression of Hematopoietic Cells*

(Received for publication, August 17, 1996, and in revised form, January 17, 1997)

Satsuki Iwase Dagger , Yusuke Furukawa §, Jiro Kikuchi , Makoto Nagai Dagger , Yasuhito Terui , Mitsuru Nakamura and Hisashi Yamada Dagger

From the Division of Hemopoiesis, Institute of Hematology, and the Department of Hematology, Jichi Medical School, Tochigi 329-04, the  Katsuta Research Laboratory, Hitachi Koki Company, Ltd., Ibaraki 312, and the Dagger  Department of Internal Medicine (Aoto) and the Department of Molecular Genetics, Institute of DNA Medicine, Jikei University School of Medicine, Tokyo 105, Japan

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
REFERENCES


ABSTRACT

E2F is a heterodimeric transcription factor that controls transcription of several growth-regulatory genes including cdc2. To investigate the mechanism of interferon-alpha (IFN-alpha )-mediated growth suppression of hematopoietic cells, we examined the effect of IFN-alpha on the expression and function of E2F using IFN-sensitive Daudi cells. Down-regulation of E2F-1, a subunit of E2F, was observed after 8 h of culture with IFN-alpha ; expression of E2F-4, another subunit of E2F, and DP-1, a heterodimeric partner of E2F, was unaffected. Gel shift assays revealed that the DNA binding activity of free E2F, which is composed of E2F-1 and E2F-4, was inhibited by IFN-alpha . In contrast, IFN-alpha did not affect the DNA binding ability of E2F-1 and E2F-4 in a complex with retinoblastoma (RB) susceptibility gene family proteins including pRB, p107, and p130. IFN-alpha could induce dephosphorylation of pRB, thereby turning active E2F-pRB complexes into transcriptional repressors. Transient chloramphenicol acetyltransferase assays revealed that the activity of the E2F-dependent cdc2 promoter was suppressed by IFN-alpha . These results suggest that the antiproliferative action of IFN-alpha is mediated through the modulation of E2F activity in two different ways: down-regulation of transcriptionally active free E2F and conversion of E2F-pRB complexes into transcriptional repressors.


INTRODUCTION

Interferons (IFNs)1 are a family of biological response modifiers with a broad spectrum of action on cellular proliferation as well as immunoregulation (1, 2). It is well known that IFNs can effectively inhibit the growth of some types of hematopoietic cells, and now they have been used as a therapeutic reagent for a variety of hematological malignancies including chronic myeloid leukemia, hairy cell leukemia, and low grade B-cell lymphomas (3). Although IFNs have an established status as a first-line drug for chronic myeloid leukemia and hairy cell leukemia, some problems remain unresolved, including drug resistance and numerous adverse effects. Insights into the molecular mechanisms of IFN action are helpful in resolving these problems. However, the mechanisms regulating the antiproliferative effect of IFNs have not been elucidated fully despite extensive investigations.

Recent investigations revealed that IFNs can induce activation of a group of IFN-stimulated gene products including a double-stranded RNA-activable protein kinase (4), the IFN regulatory factors IRF-1 and IRF-2 (5), gene 200 cluster proteins (6), and RNase L (7). Direct and indirect evidence indicates that several of these proteins may have tumor-suppressive activities. Among the growth-regulatory genes and gene products, IFNs are known to induce down-regulation of c-myc expression (8), dephosphorylation of the retinoblastoma (RB) susceptibility gene product (9), and inhibition of both the expression and activity of cyclin-dependent kinases (10-12). Although the antiproliferative effect of IFN is thought to be mediated through these events, further investigation should be required to elucidate fully the direct mechanisms of its action. For example, it is difficult to determine whether these events are the cause of the growth arrest or simply a consequence of IFN-induced failure of cell cycle progression. Furthermore, through the study of transcriptional activation in response to IFNs, the direct involvement of Jak-Stat (Janus kinases-signal transducers and activators of transcription) pathways in signal transduction of IFNs has recently been proved (13). The role of the Jak-Stat system in IFN-mediated growth suppression of hematopoietic cells is not yet clarified.

E2F is a heterodimeric transcription factor that was originally identified as an element needed for the E1A-dependent activation of a specific adenoviral E2 promoter (14). E2F is composed of each member of the E2F and DP families. Currently, five distinct E2F family members (E2F-1 to E2F-5) and three DP proteins (DP-1 to DP-3) are known (15). E2F-1 is the most characterized component of E2F and binds to DNA as a heterodimer with DP-1. Although DP-1 itself has little or no affinity for DNA, the association of E2F-1 and DP-1 leads to enhanced DNA binding and is required for E2F site-dependent transcriptional activation (16). E2F binding sites were detected in the promoter regions of many growth-responsive or growth-promoting genes such as c-myc, c-myb, cdc2, and genes for dihydrofolate reductase, thymidine kinase, DNA polymerase-alpha , and cyclin A (17). The role of E2F in the transcriptional regulation of the c-myc, dihydrofolate reductase, and cdc2 genes has been confirmed (18-20). Moreover, E2F is now considered to be a relevant target of RB family proteins including p107, p130, and RB protein (pRB) itself in their activity as growth suppressors (21). These observations strongly suggest that E2F can be a target of the action of growth-inhibitory factors such as IFNs and transforming growth factor-beta .

In this study, with this background in mind, investigations were carried out to clarify the involvement of E2F in IFN-induced growth suppression of hematopoietic cells. We have also studied the mechanisms of the inhibition of E2F activity by IFN-alpha with a special reference to its interaction with RB protein.


MATERIALS AND METHODS

Reagents

Highly purified natural IFN-alpha derived from Sendai virus-infected Nawalwa cells (22) was provided by Sumitomo Pharmaceutical Co. Ltd. (Osaka, Japan). 20 IFN-alpha components were included in this preparation with specific activities of 1.3 × 108-2.6 × 108 IU/mg of protein (22).

Cells and Culture

Burkitt lymphoma cell line Daudi was maintained in RPMI 1640 medium supplemented with 10% fetal calf serum. Cellular DNA and RNA synthesis was monitored by pulse labeling the cells for a final 1 h of the culture with 5 µCi/ml [3H]thymidine and [3H]uridine (Amersham Corp.), respectively. Cell cycle distribution was determined by analyzing the samples of 106 cells stained with propidium iodide in a flow cytometry with FACScan/CellFIT system (Becton-Dickinson, San Jose, CA).

DNA Clones

The following cDNA clones were used in this study: a 1.4-kb EcoRI-BamHI fragment of human E2F-1 cDNA (provided by Drs. William G. Kaelin, Jr., James A. DeCaprio, and David M. Livingston, Dana-Farber Cancer Institute, Boston, MA) (23); a 0.9-kb KpnI-PvuII fragment of human cdc2 cDNA (provided by Dr. Paul Nurse, Oxford University, Oxford, U. K.); a 1.8-kb ClaI-EcoRI fragment of c-myc exon 3 cDNA (Oncor Inc., Gaithersburg, MD); and a 2.2-kb EcoRI-BamHI fragment of p53 cDNA (provided by Japanese Cancer Research Resources Bank). A 1.3-kb full-length fragment of murine DP-1 cDNA and a 1.2-kb full-length fragment of human E2F-4 cDNA were generated by reverse transcription-polymerase chain reaction based on the published sequences, respectively (24, 25).

Northern Blotting

Total cellular RNA was isolated by cesium chloride centrifugation using CS120FX ultracentrifuge and S100AT5 fixed-angle rotor (Hitachi Koki, Co. Ltd.). 10-µg samples were electrophoresed in a 1% agarose gel containing 6% formaldehyde, 20 mM MOPS, 5 mM sodium acetate, and 1 mM EDTA, and blotted onto Hybond N+ synthetic nylon membranes (Amersham Corp.). The membranes were hybridized with each cDNA probe, which was labeled with [32P]dCTP (DuPont-NEN) with the oligonucleotide random priming method.

Western Blotting

Cells were washed with ice-cold TBS buffer (25 mM Tris-HCl, pH 8.0, 150 mM NaCl), and lysed in EBC buffer (50 mM Tris-HCl, pH 8.0, 120 mM NaCl, 0.5% Nonidet P-40, 100 mM sodium fluoride, 200 mM sodium orthovanadate) containing protease inhibitors. An equal amount (150 µg) of the samples was separated on a 7.5% SDS-polyacrylamide gel and transferred onto nitrocellulose filters (Bio-Rad). After blocking in TBS buffer with 4% bovine serum albumin (fraction V, Sigma), the membranes were incubated for 16 h with 1 µg/ml anti-pRB monoclonal antibody PMG3-245 (Pharmingen, San Diego). The membranes were developed as described previously (26). For c-Myc protein detection, anti-c-Myc monoclonal antibody (Pharmingen) was used as a primary antibody, and the bands were visualized by the enhanced chemiluminescence system (Amersham Corp.).

Metabolic Labeling and Immunoprecipitation

IFN-treated Daudi cells were incubated in methionine-free RPMI 1640 medium supplemented with 150 µCi/ml [35S]methionine for 3 h. Then the cells were washed three times and lysed in EBC buffer. Incorporation of [35S]methionine was monitored by a scintillation counting of the trichloroacetic acid-insoluble fraction. An equal amount (7.5 × 107 cpm) of the lysates was immunoprecipitated overnight with anti-E2F-1-specific monoclonal antibodies KH95 (Santa Cruz Biotechnology, Santa Cruz, CA) and SQ41 (Upstate Biotechnology, Lake Placid, NY). Immune complexes were collected on protein A-Sepharose beads (Pharmacia Biotech Inc.) and separated by 7.5% SDS-polyacrylamide gel electrophoresis. The signal was detected by fluorography. To monitor the efficiency of immunoprecipitation and the integrity of protein, the samples were subsequently immunoprecipitated with anti-beta -actin antibody (Oncogene Science Inc., Uniondale, NY).

Gel Retardation Assay

Nuclear extract was prepared according to the method of Dignam et al. (27). 5-µg samples were incubated with approximately 0.5 ng (10,000 cpm) of 32P-labeled DNA fragment containing the E2F binding site (5'-ATTTAAGTTTCGCGCCCTTTCTCAA-3') in the presence of 1 µg of sonicated salmon sperm DNA in a final volume of 25 µl. Incubations were carried out at room temperature for 30 min in 20 mM Hepes, pH 7.9, 0.1% Nonidet P-40, 40 mM KCl, 1 mM MgCl2, 0.1 mM EDTA, 0.5 mM dithiothreitol, and 10% glycerol. The protein-DNA complexes were resolved on a 4% polyacrylamide gel (0.15 × 16 × 20 cm; acrylamide:bisacrylamide ratio, 86:1 w/w) in 0.25 × Tris borate-EDTA buffer at 4 °C.

Double-stranded oligonucleotides containing the AP-1 binding site (5'-CTAGTGATGAGTCAGCCGGATC-3') and E2F-1 site (shown above) were used for competition assays.

For antibody perturbation experiments, 1 µg of each antibody was added to the above noted reaction mixture. Specific antibodies used in this study were as follows: anti-E2F-1, C-20; anti-E2F-4, C-108; anti-pRB, C36; anti-pRB, XZ55; anti-p107, SD9; and anti-p130, C-20 (all purchased from Santa Cruz Biotechnology except C36 and XZ55 from Pharmingen). Densitometric analysis of the results was performed by a Shimazu flying spot scanner CS-9000 (Shimazu Seisakusyo, Tokyo, Japan).

Immunoprecipitation-Deoxycholate Release Assay

Whole cell lysates of Daudi cells (5 mg) were incubated for 60 min at 4 °C with anti-pRB antibody C36 in IP-DOC buffer (20 mM Hepes, pH 7.9, 40 mM KCl, 6 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, and 0.1% Nonidet P-40) containing protease inhibitors. Immune complexes were recovered on protein A-Sepharose beads and washed three times in IP-DOC buffer. The associated proteins were released by the addition of 16 µl of 0.8% deoxycholate and then neutralized with 4 µl of 6% Nonidet P-40 (28). The supernatants (4 µl/assay) were used for E2F gel shift assays as described above.

Transient Transfection and Chloramphenicol Acetyltransferase (CAT) assay

Plasmids were introduced into Daudi cells by electroporation as described previously (29). Briefly, cells (2 × 107 cells/transfection) were resuspended in 500 µl of RPMI 1640 medium containing 20% fetal calf serum with 40 µg of linearized plasmid DNA. Electropulse was delivered at 250 V, 960 microfarads by a Gene Pulser (Bio-Rad). The cells were placed on ice for 15 min, resuspended at 5 × 105 cells/ml in RPMI 1640 containing 10% fetal calf serum, and split equally into two flasks. IFN-alpha was added into one of them at the final concentration of 100 IU/ml. Cell extracts were prepared after 24 h of the culture, and the protein concentration was determined by the Bradford method (60). CAT activity was assayed with 50 µg of each sample. CAT assays were carried out according to the standard procedure, and the activities were measured quantitatively by a liquid scintillation counting (30). The 5'-untranslated sequence of the cdc2 promoter up to nucleotide -383 relative to the transcription start site was linked to the CAT gene in pCAT-basic vector (Promega, Madison, WI) and was used as a reporter plasmid (30). pCAT-control vector (Promega), which contains SV40 promoter and enhancer sequences, was transfected simultaneously and used as a positive control. As a negative control, pCAT-basic vector containing the cdc2 promoter (up to -383) with the mutated E2F binding site was used (30). All plasmids were purified by cesium chloride gradient ultracentrifugation, linearized by appropriate restriction enzymes, and purified again by ethanol precipitation before transfection. Each result was adjusted according to the value obtained with the transfection of pCAT-control vector into corresponding cells.


RESULTS

Effect of IFN-alpha on Cell Cycle Distribution and DNA Synthesis of Daudi Cells

Among hematopoietic cells, Daudi is the most sensitive to IFN and easily arrests in G0/G1 phase of the cell cycle in response to a relatively low amount of IFN-alpha (31). We first examined the effect of IFN-alpha on cell cycle distribution and DNA synthesis of Daudi cells. The cells were seeded at an initial concentration of 5 × 105 cells/ml and grown in the absence or presence of 100 IU/ml IFN-alpha . The change of cell cycle distribution was monitored by serial analysis of the DNA histogram. The proportion of S phase was approximately 45% in untreated Daudi cells (Fig. 1A, T-0). When they were cultured with IFN-alpha , the S phase fraction was decreased gradually and reached to 21% after 24 h of culture (Fig. 1A, T-24). Conversely, approximately 60% of the cells were found to be arrested in G0/G1 phase after 24 h. Cellular DNA synthesis, as determined by [3H]thymidine uptake, correlated well with the portion of the cells in S phase, i.e. it was suppressed to be about 40% of the untreated control at 24 h (Fig. 1B). RNA synthesis was also suppressed by IFN-alpha in a similar manner (Fig. 1C). Using this culture system, we investigated the effect of IFN-alpha on E2F.


Fig. 1. Effect of IFN-alpha on proliferation of Daudi cells. Daudi cells were cultured at an initial concentration of 5 × 105 cells/ml in the absence (-) or presence (+) of 100 IU/ml IFN-alpha for 24 h. Panel A, DNA histogram was obtained by flow cytometric analysis of propidium iodide-stained cells (106 cells/sample). The percentage of S phase was calculated with the CellFIT program. Panel B, cellular DNA synthesis was monitored by pulse labeling the cells for a final 1 h of the culture with 5 µCi/ml [3H]thymidine. The data were corrected for the number of viable cells at each time point and expressed as cpm/105 cells. Panel C, cellular RNA synthesis was monitored simultaneously with the same procedure using [3H]uridine. Data shown are representative of three independent experiments.
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Effect of IFN-alpha on mRNA Expression of Major Subunits of E2F Transcription Factor, E2F-1, E2F-4, and DP-1

Total cellular RNA was isolated from Daudi cells at various time points after IFN-alpha treatment, and the expression of E2F-1, E2F-4, and DP-1 mRNA was examined by Northern blot hybridization. In agreement with our previous observation (30), both E2F-1 and DP-1 mRNA transcripts were detected readily in untreated Daudi cells, reflecting the proliferative state of the cells. As shown in Fig. 2A, the amount of E2F-1 mRNA decreased significantly after 8 h of culture with IFN-alpha . It is of note that [3H]thymidine incorporation, a sensitive marker of cell proliferation, was not suppressed at this time point (Fig. 2B). This clearly indicates that down-regulation of E2F-1 mRNA is not a simple consequence of the growth arrest and may have a causative role in IFN-induced growth arrest. In striking contrast, the E2F-4 mRNA level was unchanged during this culture period. DP-1 mRNA expression was also unaffected by the IFN treatment, although a minor decrease was observed after 8 h (Fig. 2A).


Fig. 2. Effect of IFN-alpha on mRNA expression of major subunits of E2F transcription factor, E2F-1, E2F-4, and DP-1. Daudi cells were cultured in the absence (-) or presence (+) of 100 IU/ml IFN-alpha for 24 h. Panel A, total cellular RNA was isolated at the indicated time points and subjected to sequential analysis for E2F-1, E2F-4, and DP-1 mRNA expression. Ethidium bromide-stained 28 S rRNA is shown as a loading control. Panel B, cell proliferation was monitored simultaneously by an [3H]thymidine incorporation assay as described in the legend of Fig. 1. The results are shown as the percent of the untreated control (([3H]thymidine uptake of IFN-alpha -treated cells)/([3H]thymidine uptake of the untreated cells at the same time point) × 100). Data shown are representative of three independent experiments.
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Effect of IFN-alpha on De Novo Synthesis of E2F-1 Protein

Next, we examined the effect of IFN-alpha on de novo synthesis of the E2F-1 gene product by metabolic labeling and subsequent immunoprecipitation. IFN-treated Daudi cells were pulse labeled for 3 h with [35S]methionine, and whole cell lysates were subjected to immunoprecipitation with anti-E2F-1-specific monoclonal antibodies to monitor the amount of a newly synthesized E2F-1 protein. As a control, the samples were subsequently immunoprecipitated with anti-beta -actin antibody. In accordance with the marked reduction of the amount of mRNA transcript, a newly synthesized E2F-1 protein was barely detected after 8 h of the treatment with IFN-alpha , whereas the amount of beta -actin was unaffected (Fig. 3). A minor increase in E2F-1 protein was observed in untreated cells after 8 h of culture. This may be the result of a small increase in the amount of E2F-1 mRNA (see Fig. 2A) during spontaneous cell growth (see Fig. 1B).


Fig. 3. Effect of IFN-alpha on de novo synthesis of E2F-1 protein. Daudi cells were cultured in the absence (-) or presence (+) of 100 IU/ml IFN-alpha for 8 h. The cells were pulse labeled with [35S]methionine for 3 h. An equal amount (7.5 × 107 cpm) of the lysates was immunoprecipitated with anti-E2F-1-specific monoclonal antibodies and subsequently with anti-beta -actin antibody. Immune complexes were separated by 7.5% SDS-polyacrylamide gel electrophoresis, and the signal was detected by fluorography. 14C-Labeled standard proteins (Amersham Corp.) were used as molecular weight markers (M.W.). The position of each band is shown on the left. Data shown are representative of three independent experiments.
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Effect of IFN-alpha on DNA Binding Activity of E2F

To investigate whether this reduction of the amount of E2F-1 protein affects the transcriptional activity of E2F, we first examined the DNA binding ability of E2F in IFN-treated Daudi cells. Nuclear extracts were prepared at various time points after IFN treatment and tested for E2F activity by gel retardation assays using an E2F consensus oligonucleotide from the adenovirus E2 promoter as a probe (32). As shown in Fig. 4A, when the nuclear extract from untreated Daudi cells was used, multiple bands with retarded mobilities were resolved on a 4% native polyacrylamide gel. By oligonucleotide competition assays, seven distinct bands (designated as A to G) were found to be specific E2F complexes. These bands were considered to represent the baseline E2F activity of proliferating Daudi cells. Two other fast migrating bands were considered to be nonspecific, since they were not eliminated by the addition of 100 molar excess of unlabeled E2F oligonucleotide (indicated by stars in Fig. 4). Then we performed antibody perturbation experiments to identify the nature of each complex. Specific antibodies against E2F-1, E2F-4, pRB, p107, and p130 were included in the reaction mixture, and the E2F gel shift assay was carried out as above (Fig. 4B). The results were quantitated by densitometric analysis and are summarized in Table I. Given that any specific antibody against RB family proteins (pRB, p107, and p130) did not affect the intensity of the bands E, F, and G, these complexes represent so-called free E2F. Furthermore, anti-E2F-4- but not anti-E2F-1-specific antibody could eliminate band E completely, indicating that this band is composed mainly of E2F-4 (Fig. 4B). On the other hand, two faster migrating free E2F bands (F and G) were not supershifted by anti-E2F-4 antibody, but the signal intensities were slightly but significantly decreased in the presence of anti-E2F-1 antibody (Fig. 4B and Table I). This suggests that complexes F and G contain E2F-1 and other components of the E2F family such as E2F-2 and E2F-3. Four other bands (A to D) were found to be complexes of E2F and RB family proteins. Anti-pRB antibody effectively supershifted both bands C and D, and the former was eliminated by anti-E2F-4. These results therefore indicate that band C represents a complex containing E2F-4 and pRB. In contrast, band D was not abolished by either anti-E2F-1 or anti-E2F-4, suggesting that other E2F family members such as E2F-2 and E2F-3 form a complex with pRB in D. Similarly, the complex A was found to contain E2F-4, p107, and p130. Complex B disappeared with the addition of anti-p130, but it was not affected by either anti-E2F-1 or anti-E2F-4. Thus, complex B may be composed of p130 and a member of the E2F family other than E2F-1 and E2F-4. E2F-5 is a strong candidate as the E2F molecule present in this complex, as suggested by recent investigations (33, 34).


Fig. 4. Characterization of E2F complexes present in Daudi cells by gel retardation assays with antibody perturbation. Panel A, nuclear extracts were prepared from untreated Daudi cells and incubated with 32P-labeled DNA fragment containing E2F binding sites. The protein-DNA complexes were resolved on a 4% polyacrylamide gel in 0.25 × Tris borate-EDTA buffer. Double-stranded oligonucleotides containing the AP-1 binding site and E2F binding site were used for competition assays. The bands indicated by stars were found to be nonspecific. Panel B, E2F gel shift assays were performed in the absence or presence of antibodies against E2F-1, E2F-4, pRB, p107, and p130. For quantitation of the results, see Table I. Data shown are representative of three independent experiments.
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Table I. Quantitation of the effect of specific antibodies on signal intensities of the E2F complexes

The signal intensity of each band was quantitated by densitometric analysis of the autoradiogram shown in Fig. 4B with that of upper nonspecific band (indicated by an asterisk) setting at 1.0.  The signal intensity of each band was quantitated by densitometric analysis of the autoradiogram shown in Fig. 4B with that of upper nonspecific band (indicated by an asterisk) setting at 1.0. 
Additions

None  alpha E2F-1  alpha E2F-4  alpha pRB  alpha p107  alpha p130
A 0.58 0.72 0.03a 1.22 NDa,b 0.03a
B 0.13 0.20 0.62 0.48 0.03a
C 0.48 0.48 0.18a NDa,b 0.36 0.31
D 0.31 0.44 0.39 ND a,b 0.28 0.30
E 1.46 1.20 0.19a 1.28 1.18 1.39
F 2.35 1.78a 2.42 2.05 2.20 2.13
G 0.59 0.12a 0.45 0.46 0.38 0.61
* 1.0 1.0 1.0 1.0 1.0 1.0

a More than 20% decrease in intensity compared with the control (None).
b ND, not detected (under the detection limit).

The effect of IFN-alpha on E2F activity was analyzed next by comparing the intensities of these bands before and after treatment. Fig. 5A displays the representative result of gel retardation assays with nuclear extracts from Daudi cells isolated after 0, 8, and 24 h of culture with 100 IU/ml IFN-alpha . The intensity of bands F and G (corresponding to free E2F-1) was decreased significantly by IFN-alpha after 8 h of IFN treatment, which is consistent with the reduction of E2F-1 mRNA and protein. After 24 h, the amounts of free E2F-1 were reduced to approximately 25% of the untreated control by densitometric comparison. The abundance of the band E, which corresponds to free E2F-4, was unaltered at 8 h but was reduced markedly after 24 h of treatment (Fig. 5A). IFN also reduced the amounts of E2F-pRB complexes (C and D) and the E2F-4-p107 complex (A). The decrease in the E2F-p107 complex may correspond to the reduction of S phase cells by IFN, since this complex was reported to be preferentially formed in S phase (35).


Fig. 5. Effect of IFN-alpha on DNA binding activity of E2F. Panel A, nuclear extracts were prepared after 0, 8, and 24 h of the culture with 100 IU/ml IFN-alpha . E2F activity was examined by gel shift assays as described in the legend of Fig. 4. Specific E2F complexes are indicated by letters A to G, and nonspecific bands are indicated by stars. Data shown are representative of three independent experiments. Panel B, pRB-associated proteins were immunoprecipitated from whole cell lysates of Daudi cells before (lanes 1 and 2) and after (lanes 3-6) the IFN treatment, and the E2F gel shift assay with antibody perturbation was carried out after deoxycholate release of the supernatants. E2F activity was detected in the absence (lanes 1 and 3) or presence of antibodies against E2F-1 (lane 4), E2F-4 (lane 5), and cold competitor (lanes 2 and 6).
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Next, we performed an immunoprecipitation-deoxycholate release assay to confirm that E2F-4 really made a complex with pRB and also to see more precisely the effect of IFN on the E2F-4-pRB complex. pRB-associated proteins were immunoprecipitated from whole cell lysates of Daudi cells before and after the IFN treatment, and E2F gel shift assays with antibody perturbation were carried out after deoxycholate release of the supernatants. As shown in Fig. 5B, two bands (designated as X and Y) were observed, and both of them were eliminated completely by the cold competitor (lanes 2 and 6). Anti-E2F-4 antibody could reduce the intensity of band X, indicating that E2F-4 really is present in E2F-pRB complexes (lane 5). Similarly, E2F-1 was shown to be present in band Y (lane 4). There was no difference in the amounts of pRB-bound E2F between pre- and post-treatment samples (compare lanes 1 and 3) despite the decrease in the intensities of E2F-pRB complexes (C and D in Fig. 5A) in gel retardation assays. This suggests that E2F molecules associated with a single pRB molecule are not decreased even after the IFN treatment. This is fully consistent with the previous report by Melamed et al. (36), wherein inhibition of E2F activity started after 8 h of treatment with interleukin-6 or IFN, but it was not eliminated completely even after 24 h. In keeping with the previous assumption that E2F binds preferentially to unphosphorylated pRB (37, 38), this may be explained by the change in phosphorylation status of pRB. Therefore, we then examined the effect of IFN-alpha on the amount and phosphorylation status of RB protein. This may be also helpful in characterizing further the function of E2F-pRB complexes, since the activity of E2F was regulated mainly by its association with pRB.

Effect of IFN-alpha on Phosphorylation Status of pRB

Whole cell lysates were prepared from Daudi cells at various time points after the IFN treatment and were subjected to immunoblot analysis for pRB expression. Previous reports have documented that RB protein is unphosphorylated in G0/G1 phase of the cell cycle and is specifically phosphorylated at the G1/S boundary; phosphorylated pRB is dominant throughout the S and G2/M phases (39, 40). Only underphosphorylated RB protein can function as a suppressor of cell growth. These two functionally distinct forms of pRB are clearly distinguishable on a 7.5% polyacrylamide gel.

As shown in Fig. 6A, RB protein was present almost exclusively in heavily phosphorylated forms in untreated Daudi cells, reflecting the active proliferative status of the cells. IFN-alpha induced the accumulation of an underphosphorylated form of pRB after 24 h of treatment (Fig. 6A). Recently, Weintraub et al. (41) reported that unphosphorylated RB protein, upon binding to E2F, switches E2F from a transcriptional activator to a repressor. Thus, E2F-pRB complexes present after the IFN treatment may function as a suppressor of cell growth. Taken together, these results indicate that IFN-alpha suppresses transcriptional activity of E2F in two ways: through reduction of transcriptionally active free E2Fs (E2F-1 and E2F-4) and through induction of unphosphorylated pRB to change E2F-pRB complexes to transcriptional repressors.


Fig. 6. Effect of IFN-alpha on phosphorylation status of RB protein. Panel A, whole cell lysates were isolated from IFN-alpha -treated (+) and untreated control (-) Daudi cells at the indicated time points and subjected to immunoblotting with anti-pRB monoclonal antibody. The positions of underphosphorylated RB protein (pRB) and phosphorylated RB protein (pRBphos) are indicated. Panel B, the growth-inhibitory effect of IFN-alpha was monitored simultaneously by [3H]thymidine uptake as described in the legend of Fig. 1. Data shown are representative of three independent experiments.
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Effect of IFN-alpha on E2F-dependent transcription of the cdc2 Gene

We next investigated the effect of IFN-alpha on the transactivating ability of E2F in vivo. For this purpose, we chose to examine whether IFN-alpha could suppress the activity of the cdc2 promoter by transient CAT assay. A 467-base pair fragment of the 5'-flanking region of the cdc2 gene (-383 to +87) was linked to the CAT gene in pCAT-basic vector and used as a reporter plasmid (hereinafter designated as pCAT-5'-cdc2). In our previous study, it was shown that this segment had a strong promoter activity that was dependent on the binding of E2F at nucleotide positions -124 to -117 (30). A nonbinding mutation was introduced into the E2F binding site of pCAT-5'-cdc2 (5'-TTTCGCGC-3' to 5'-TTTCGATC-3') and used as a negative control. The plasmid containing SV40 promoter-driven CAT (pCAT-control) was transfected simultaneously into corresponding cells and used as a positive control. These plasmids were transiently transfected into Daudi cells by electroporation, and the cells were split equally into two flasks. IFN-alpha was added into one of them, and the cells were cultured for 24 h and harvested for measurement of intracellular CAT activity. The promoter activity of pCAT-control was independent of E2F and was shown to be unaffected by IFN-alpha (data not shown). In contrast, IFN-alpha could suppress the CAT activity of pCAT-5'-cdc2 to 74 ± 4% of the untreated control in four independent experiments (p < 0.01 by Student's t test, Fig. 7, left panel). As anticipated, IFN-alpha could not inhibit the promoter activity of the mutant plasmid lacking a functional E2F binding site (Fig. 7, right panel). This indicates that E2F-dependent promoter activity was regulated negatively by IFN-alpha in vivo. This result facilitated further examination of the expression of the genes whose promoter contains functional E2F binding sites in IFN-treated Daudi cells.


Fig. 7. Inhibition of the E2F-dependent cdc2 promoter activity by IFN-alpha . A 467-base pair fragment of the 5'-flanking region of the cdc2 gene (-383 to +87) was linked to the CAT gene in pCAT-basic vector (Wild Type) and transfected into Daudi cells by electroporation. A nonbinding mutation was introduced into the E2F site of the same plasmid and used as a negative control (Mutant). The cells were split equally into two fractions, and IFN-alpha (100 IU/ml) was added into one of them (+). Cell extracts were prepared after 24 h, and 50 µg of each sample was used for CAT assay. Relative CAT activity was calculated as the percentage of the value obtained in the absence of IFN (-). The mean ± S.D. (bar) of four independent experiments is shown. Statistical analysis was performed with Student's t test.
[View Larger Version of this Image (65K GIF file)]

IFN-alpha Could Suppress the Expression of c-myc and cdc2 mRNA

Both c-myc and cdc2 had functional E2F binding sites in their promoter regions, and their transcription was known to be regulated by E2F (18, 20, 30). The effect of IFN-alpha on expression of c-myc and cdc2 mRNAs was investigated in IFN-treated Daudi cells. Expression of p53 mRNA, whose transcription is independent of E2F, was examined simultaneously as a negative control. As shown in Fig. 8A, IFN-alpha could effectively inhibit the expression of c-myc and cdc2, whereas p53 mRNA expression was unaffected. IFN-induced suppression of cdc2 mRNA occurred after 12 h of treatment, whereas down-regulation of c-myc mRNA became evident after 72 h. The inhibition of cdc2 and c-myc mRNA was apparently preceded by the suppression of E2F activity. This is entirely compatible with the notion that IFN-induced suppression of these growth-related genes is mediated at least in part through the inhibition of E2F activity. Down-regulation of these genes may contribute somewhat to the growth arrest of Daudi cells, although many other factors should be involved in this process (Fig. 8B).


Fig. 8. Effect of IFN-alpha on expression of c-myc and cdc2 mRNA transcripts in Daudi cells. Panel A, Daudi cells were cultured in the absence (-) or presence (+) of 100 IU/ml IFN-alpha , and total RNA was isolated after 0, 24, 48, and 72 h. The expression of c-myc, cdc2, and p53 mRNA transcripts was analyzed by Northern blotting. Panel B, the growth-inhibitory effect of IFN-alpha was monitored simultaneously by [3H]thymidine uptake as described in the legend of Fig. 1. Data shown are representative of three independent experiments.
[View Larger Version of this Image (54K GIF file)]


DISCUSSION

Two decades ago the antiproliferative activity of IFNs was first identified (42). However, the mechanism of IFN-mediated growth suppression is not fully understood. Previous investigations have shown that IFN causes G0/G1 arrest through the reduction of c-myc proto-oncogene (8) and dephosphorylation of pRB (9). Regarding the cell cycle-regulatory elements, down-regulation of cyclin A and cdk2 mRNA transcripts by IFN-alpha was observed recently (11). Inhibition of cyclin E- and cyclin D1- but not cyclin A-dependent cdk2 kinase activity was also reported (12). Although the action of IFN is thought to be mediated through these events, it is not clear whether or not this is a direct action. It is possible that some of these events were the result of IFN-induced growth arrest. Given the fact that IFN is now used widely as a therapeutic reagent, further investigation should be required to elucidate fully the direct mechanism of its action for better clinical application.

In this work, we investigated the effect of IFN-alpha on transcription factor E2F, one of the most important regulators of G1/S transition. We found that expression of E2F-1 mRNA, which encodes a major subunit of E2F, was reduced markedly after 8 h of the culture with IFN-alpha , whereas expression of DP-1, another subunit of E2F, was unaffected. Down-regulation of E2F-1 mRNA was faster than that of any other genes whose expression was regulated negatively by IFN (11). Because of the inhibition of E2F-1 mRNA expression, a newly synthesized E2F-1 protein became undetectable after 8 h, which was accompanied by the suppression of its DNA binding activity. In addition, IFN-alpha also diminished the DNA binding ability of E2F-4, another member of the E2F family with an affinity to the same binding site as E2F-1. In contrast to E2F-1, IFN-induced suppression of E2F-4 activity is suspected to be post-transcriptional, since E2F-4 mRNA expression was not inhibited by IFN. Gel shift assays revealed that IFN could especially repress the DNA binding ability of E2F-1 and E2F-4 without a complex with RB family proteins (free E2F). Since it has been established that both free E2F-1 and free E2F-4 activate transcription of many growth-promoting genes (43, 44), suppression of free E2F activity may be implicated in IFN-induced cell growth arrest. By contrast, IFN did not reduce the amounts of E2F in a complex with RB protein in immunoprecipitation-deoxycholate release assays. Accumulation of an underphosphorylated, growth-suppressive form of RB protein was induced simultaneously by the IFN treatment. Recently, it has been shown that unphosphorylated RB protein, upon binding to E2F, switches E2F from a transcriptional activator to a repressor (41). Therefore, E2F-pRB complexes present after the IFN treatment may function as suppressors of cell growth. Taken together, these results indicate that IFN-alpha suppresses E2F activity in two different ways: through the reduction of transcriptionally active E2F-1 and E2F-4 and through the induction of unphosphorylated pRB to generate transcriptional repressor complexes containing E2F. As a result, E2F-dependent cdc2 promoter activity was indeed suppressed by IFN-alpha in vivo. The suppression of E2F activity was followed by down-regulation of the growth-regulatory genes such as c-myc and cdc2 whose transcription is controlled by E2F.

Recent studies using E2F-1 have demonstrated a central role for E2F in cell cycle regulation. Expression of E2F-1 can prevent quiescence upon serum deprivation and can induce quiescent cells into S phase (45). Recombinant adenovirus-mediated overexpression of E2F-1 can activate transcription of DNA synthesis-related and G1/S-regulatory genes such as those for DNA polymerase-alpha , thymidylate synthase, proliferating cell nuclear antigen, cyclin A, and cdc2 in REF52 cells without serum stimulation (46). Moreover, infection of Mv1Lu mink lung epithelial cells with adenovirus vector containing E2F-1 cDNA overcomes transforming growth factor-beta -mediated growth suppression (47). Similarly, overexpression of E2F-1 can bypass a G1 arrest caused by the inhibition of G1-specific cyclin-dependent kinase activity and by gamma -irradiation (48). Recently, Guy et al. (49) reported that overexpression of E2F-1 in transgenic mouse under the control of PF4 promoter resulted in the block of megakaryocytic maturation and caused abnormal proliferation of megakaryocytes. Their study clearly demonstrates the role of E2F-1 in cell proliferation and differentiation in vivo. From these observations, E2F-1 is thought to be a general target for both growth-stimulatory and -inhibitory signals. Our present finding is in line with this view and may provide a new concept on signaling pathways of IFN-mediated growth suppression.

Convincing evidence has accumulated suggesting that E2F is a relevant target of the action of RB protein as a growth suppressor. The interaction of pRB, especially in its underphosphorylated form, with E2F results in a switch of E2F from a transcriptional activator to a repressor (41). The ability of pRB to interact with E2F correlates directly with the ability of pRB to arrest cells in G0/G1 (50). Mutant RB protein that lacks the ability to cause a growth arrest also lacks the ability to bind E2F and to inhibit E2F-dependent transcription. In the G0/G1 phase of the cell cycle, unphosphorylated pRB binds directly to E2F and inhibits E2F-dependent transcription (37, 38). As cells pass the G1/S boundary by growth stimulation, pRB becomes phosphorylated, and E2F is released. The unbound free E2F (mainly E2F-1 and E2F-4) is presumed to be transcriptionally active. Conversely, some negative growth factors such as transforming growth factor-beta can induce dephosphorylation of pRB, thereby inhibiting E2F activity and leading cells to G1 arrest (26, 51). The accumulation of the underphosphorylated, growth-suppressive form of RB protein and its negative effect on E2F activity might be important for IFN-mediated growth suppression. In this study, we demonstrated that E2F-4, in addition to E2F-1, formed a specific complex with pRB in Daudi cells. E2F-4 is a recently identified member of the E2F family which makes a complex with RB family proteins (25). E2F-4-p107 and E2F-4-p130 complexes are present in early to mid G1 phase of the cell cycle and are shown to act as repressors of E2F site-directed transcription of B-myb (52) and cdc2 (53). In previous reports, E2F-4 was shown to bind preferentially p107 and p130 and was believed not to bind pRB (43, 44). This is somewhat contradictory to our present observation that E2F-4 is present in a complex with pRB. However, Ikeda et al. (54) recently described that E2F-4 was the major species of E2F in quiescent fibroblasts and in differentiated HL-60 cells, where E2F-4 made complexes with unphosphorylated pRB and p130 to induce cell cycle arrest. They claimed that it is the ratio of free E2F to pRB-bound E2F which is critical in the decision to pass through the G1/S transition. Our finding is fully consistent with their assertion and demonstrates that the relative increase in the ratio of pRB-bound E2F to free E2F, mainly because of the decrease in free E2F, is linked to IFN-induced suppression of E2F-mediated transcription and cell cycle arrest.

How does IFN-alpha repress E2F-1 mRNA expression? Transcriptional activation in response to IFN-alpha is known to be mediated through the activation of a multiprotein DNA-binding complex described as IFN-stimulated gene factor 3 (ISGF3) (55). The components of ISGF3 were purified and are recognized now as Stat1, Stat2, and p48 (56). Tyrosine phosphorylation of Stat proteins by IFN-alpha /beta receptor-associated Jak kinases is necessary for activation of ISGF3. The phosphorylated Stat proteins move to the nucleus and bind specific DNA elements called IFN-stimulated response elements to direct transcription (57). However, there is no binding site for ISGF3 in the promoter region of the E2F-1 gene according to recent reports (58, 59). Therefore, direct involvement of the Jak-Stat signaling pathway in IFN-induced suppression of E2F-1 mRNA transcription is unlikely. Recent investigations suggest that transcription of the E2F-1 gene is under autoregulatory control through the E2F binding sites in its promoter (58, 59). Accordingly, transcription of E2F-1 is repressed when E2F binds to the promoter region of the E2F-1 gene in a complex with underphosphorylated pRB, since this complex acts as a transcriptional repressor. Although this is a plausible explanation, down-regulation of E2F-1 mRNA started apparently prior to dephosphorylation of pRB in our experiments. It is possible that other molecules in the E2F family play a role in IFN-mediated inhibition of E2F-1 mRNA. E2F-5 may be a good candidate because it binds to the RB family protein p130 and is the predominant E2F complex in the early phases of the cell cycle (G0 to mid G1) (34, 35). To understand the precise mechanism of the repression of E2F-1 expression requires a study of the effect of IFN-alpha on other members of the E2F family. Detailed analysis of these subjects is currently under way in our laboratory.

Finally, we have to mention the discrepancy between our study and the previous one by Melamed et al. (36) regarding the expression patterns of c-Myc after IFN treatment. As shown in Fig. 8, down-regulation of c-myc mRNA was observed after 72 h of IFN treatment in our study. However, they described that c-Myc protein levels were inhibited at 8 h in Daudi cells (36). Thus, we also performed Western blot analysis of c-Myc expression in IFN-treated Daudi cells. Consistent with their result, the c-Myc protein was down-regulated after 24 h of culture with IFN in our system (data not shown), whereas c-myc mRNA expression was unaffected at this time point. This suggests that there might be multiple distinct mechanisms for IFN-induced suppression of c-Myc including E2F-dependent inhibition of mRNA transcription and post-translational modification of the protein levels. The precise mechanism of the latter is at present unknown.


FOOTNOTES

*   This work was supported in part by a grant-in-aid from the Ministry of Education Science and Culture of Japan and by a grant from the Ichiro Kanehara Foundation (to Y. F.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
§   To whom correspondence should be addressed: Division of Hemopoiesis, Institute of Hematology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-04, Japan. Tel.: 81-285-44-2111; Fax: 81-285-44-7501.
1   The abbreviations used are: IFN, interferon; RB, retinoblastoma; Jak-Stat, Janus kinases-signal transducers and activators of transcription; kb, kilobase; MOPS, 4-morpholinepropanesulfonic acid; CAT, chloramphenicol acetyltransferase; ISGF3, IFN-stimulated gene factor 3.

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