Binding of Upstream Stimulatory Factor and a Cell-specific Activator to the Calcitonin/Calcitonin Gene-related Peptide Enhancer*

(Received for publication, February 21, 1997, and in revised form, May 7, 1997)

Thomas M. Lanigan Dagger and Andrew F. Russo Dagger §

From the Dagger  Molecular Biology Program, § Department of Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52242

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES


ABSTRACT

The calcitonin/calcitonin gene-related peptide (CT/CGRP) gene is selectively transcribed in thyroid C cells and neurons. We have previously shown that the rat CT/CGRP cell-specific enhancer is synergistically regulated by a helix-loop-helix (HLH) protein and the OB2 octamer-binding protein. In this report, we show that the HLH-OB2 enhancer is required for full promoter activity, even in the context of other HLH elements. Since this enhancer appears to be a major controlling element, we have characterized the HLH and OB2 DNA binding proteins. We have identified the major HLH complex as a heterodimer of the ubiquitous upstream stimulatory factor (USF)-1 and USF-2 proteins. USF bound the enhancer with a reasonably high affinity (KD 1.6 nM), comparable to other genes. Characterization of a series of mutations revealed that a portion of the HLH motif is also recognized by OB2 and confirmed that HLH activity requires OB2. We have shown that OB2 is a single DNA binding protein based on UV cross-linking studies. The 68-kDa protein-DNA complex was detected only in C cell lines, including a human C cell line that has robust HLH-OB2 enhancer activity. These results suggest that the calcitonin/CGRP gene is controlled by the combinatorial activity of a ubiquitous USF HLH heterodimer and an associated cell-specific activator.


INTRODUCTION

The calcitonin/calcitonin gene-related peptide (CT/CGRP)1 gene encodes the hormone CT and the neuropeptide CGRP (1). CT lowers serum calcium levels during calcium homeostasis and is used as a therapeutic agent to maintain bone calcium in certain types of osteoporosis and Paget's disease (2, 3). CGRP has pleiotrophic effects but has been best characterized as a potent vasodilatory neuropeptide (4, 5). Elevated CGRP levels have been detected in some cardiovascular disorders (5) and in vascular headaches (6). The CT/CGRP gene is transcribed in a large number of neurons of the peripheral and central nervous systems and in the neuroendocrine thyroid C cells. Interestingly, thyroid C cell lines and cultured C cells have a more neuronal phenotype that includes neurofilament expression, serotonergic properties, and CGRP production (7-9). This acquisition of neuronal properties is consistent with the common origin of C cells with serotonergic enteric neurons from a vagal sympathoadrenal progenitor in the neural crest (10).2 Consequently, thyroid C cell lines can be used as a model to study the transcriptional regulation of CT/CGRP in both C cells and neurons.

Cell-specific transcription of the CT/CGRP gene is controlled by a distal cell-specific enhancer. Transgenic mice containing 1.3 or 1.7 kilobase pairs of flanking DNA express reporter genes in thyroid C cells and peripheral neurons (11, 12). Both the rat and human CT/CGRP distal enhancers contain several helix-loop-helix (HLH) binding sites that contribute to enhancer activity (11, 13-16). Additional sequences near the promoter are responsive to signal transduction pathways induced by cAMP (17, 18), nerve growth factor (19), and activated Ras (20). We have found that the rat CT/CGRP enhancer requires not only the HLH factor but also a cell-specific protein that binds an adjacent octamer motif (16). Synergism between the HLH protein and the octamer-binding protein, referred to as OB2, is required for activity of the enhancer (designated as HLH-OB2). This type of combinatorial control is becoming an increasingly common theme in gene transcription (21).

Since the CT/CGRP HLH complex had been detected from both CT/CGRP expressing and non-expressing cell lines (16), this suggested that ubiquitous HLH proteins might recognize the HLH-OB2 enhancer. The ubiquitous E12/E47, ITF-2, and USF proteins have been implicated in combinatorial control with other proteins, including cell-specific factors. The E12/E47 and ITF-2 proteins have been shown to functionally interact with homeodomain proteins to help direct cell-specific gene expression (22-24). The USF proteins were initially identified as upstream stimulatory factors that control the adenovirus major late promoter (25-27). Since then, USF binding sites have been found in a number of cellular genes (28-33). USF often consists of a heterodimer of the ubiquitously expressed 43-kDa USF-1 and the 44-kDa USF-2 gene products, although homodimers can also bind DNA (34, 35). Furthermore, USF has been shown to work in combination with other factors (32, 33, 36-39). While we at first did not consider the USF proteins as candidates since the central dinucleotide of the CT/CGRP HLH motif does not fit the consensus USF binding site, USF has been shown to bind and transactivate degenerate sites, including one identical to the CT/CGRP enhancer (28, 29, 33, 40, 41).

In this report, we have demonstrated the functional importance of the CT/CGRP HLH-OB2 enhancer in the context of full promoter sequences containing other regulatory elements, including other HLH motifs, and have characterized the DNA binding proteins. We found that a heterodimer of USF-1 and USF-2 proteins comprises the major HLH binding complex. The USF binding site was shown to overlap the site recognized by the OB2 protein. OB2 consists of a single 68-kDa cell-specific polypeptide that was identified in rat and human C cell lines. These results demonstrate that the CT/CGRP HLH-OB2 enhancer, a key regulatory element of the CT/CGRP gene, is bound by the ubiquitously expressed USF HLH proteins and a cell-specific protein.


EXPERIMENTAL PROCEDURES

Cell Culture

The CA77 thyroid C cell line was maintained in Dulbecco's modified Eagle's medium (DMEM) (low glucose)/Ham's F12 (1:1), 10% fetal bovine serum (FBS) (Hyclone). For the other cell lines, the media were as follows: 44-2C, DMEM (high glucose), 10% equine serum, 0.1% L-glutamine; TT, minimum essential medium-alpha , 10% FBS; COS-7, DMEM (high glucose), 10% FBS; B103, DMEM (low glucose), 10% FBS; HeLa, Ham's F12, 10% FBS; GH3, DMEM (low glucose), 2.5% FBS, 15% equine serum; Rat-1, DMEM (high glucose), 10% bovine calf serum (Hyclone). Penicillin (100 units/ml) and streptomycin (100 µg/ml) were added to all media. Cells were incubated at 37 °C in 7% CO2.

Cell Transfections and Luciferase Assays

The 1250-bp CT/CGRP plasmids contain a fragment from the KpnI site at -1250 bp to an artificial HindIII site +21 bp (16). The fragment was cloned into both the pSDK-lacZ vector provided by Dr. J. Rossant and the pGL3 luciferase vector (Promega). The herpes simplex thymidine kinase (TK) promoter (105 bp) and the 205-bp BglII CT/CGRP enhancer (-1125 to -920-bp) fragment upstream of the TK promoter plasmids have been described (42). The 1125-920 + BamHI-TK-luc plasmid was constructed by inserting an 8-bp BamHI linker (5'cggatccg3') (New England Biolabs) into the unique PvuII site of 1125-920-TK-luc. This yielded the enhancer sequence 5'CAGcggatccgCTGTGCAAT3', which contains a disrupted HLH motif with a reconstituted OB2 site. The 1250 CT/CGRP + BamHI-lacZ plasmid was constructed by replacing the BglII fragment (-1125 to -920 bp) with the BglII fragment containing the BamHI linker at the PvuII site. The CT/CGRP HLH-OB2 enhancer (-1043 to -1025 bp) plasmids (HO-TK-luc) contained either two or four tandem inserts in a (+)(+) or (-)(+)(-)(+) orientation, respectively. Similar activities were seen with 2 to 4 elements in either orientation. The HO + A-TK-luc reporter contains four copies of the HO + A element, all in the minus orientation and the HLHm1-TK-luc reporter contains 2 copies of the HLHm1 element in the plus orientation. These plasmids were constructed from annealed oligonucleotides containing BamHI ends ligated into the TK-luciferase plasmid, as described (16). CA77 and COS-7 cells were transfected by electroporation as described (42), except that COS-7 cells were electroporated at 260 V. The transfected cells from a single cuvette were grown on a 60-mm dish for 16-24 h. The cells were harvested using the reporter buffer from Promega and then assayed for luciferase and beta -galactosidase activity using reagents from Promega and Tropix Inc., respectively. Protein concentrations were determined by Bradford assays (Bio-Rad). To compare results from different experiments, the activities were normalized to an internal standard as indicated in the figure legends. In some experiments, the standard was the parental TK-luciferase plasmid, and in other experiments, a co-transfected plasmid containing a different reporter (luciferase or lacZ) under control of the cytomegalovirus (CMV) promoter was also included to allow for normalization. The CMV-luciferase and CMV-lacZ plasmids have been described (15, 42).

Electrophoretic Mobility Shift Assay

The electrophoretic mobility shift assays using the CT/CGRP HLH-OB2 enhancer element as a probe were performed essentially as described (42). Briefly, the probe was prepared by annealing 10 pmol of complementary CT/CGRP HLH-OB2 enhancer oligonucleotides with overhanging BamHI ends (lowercase) (5'gatccGGCAGCTGTGCAAATCCTg3'; 5'gatccAGGATTTGCACAGCTGCCg3') and labeled with [32P]dATP using Klenow DNA polymerase (16). All oligonucleotides used for the binding assays, except for the blunt-ended AP4, octamer and USF consensus oligonucleotides, contain the overhanging BamHI ends, which were filled with Klenow polymerase prior to use as competitors or probes. The binding reactions contained 0.02 pmol of labeled probe (50,000 cpm), 3 µg, unless otherwise noted, of nuclear extract prepared by a modified Dignam protocol (16), binding buffer (10 mM Tris, pH 7.5, 5% glycerol, 50 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol), poly(dI-dC) (0.1 µg) (Boehringer Mannheim), and 0.1 pmol of an unrelated double-stranded oligonucleotide (5'-GATCCACTATGTCTAGAG-3'). Omission of this unrelated oligonucleotide has subsequently been shown not to have any effect on the mobility shift complexes. Competitor DNAs and peptides were preincubated for 10 min on ice with the nuclear extract before addition of the probe. Antibodies were incubated 10-15 min after the addition of the probe, unless otherwise noted. Rabbit polyclonal IgG antibodies raised against USF-1 (C-20), USF-2 (N-18), USF-2 (C-20), or E12 (E2A.E12) (V-18) HLH proteins, or against CREB-binding protein (CBP) (A-22) were all obtained from Santa Cruz Biotechnology. In addition, the mammalian achaete-scute homolog 1 (MASH-1) and ITF-2 rabbit polyclonal antisera were provided by Dr. T. Brennan, and the monoclonal Pan (equivalent to human E47/E12) antiserum was provided by Dr. C. Nelson (43). All reactions were incubated for 10 min on ice before addition of 4 µl of 20% Ficoll dyes or 3 µl of 50% glycerol dyes, and then resolved by electrophoresis through a 6% (unless otherwise noted) non-denaturing polyacrylamide gel (1:29 bis/acrylamide), as described (16), and exposed to film overnight. Data were quantitated directly from dried gels using an InstantImager (Packard).

UV Cross-linking

The UV cross-linking experiments using modified CT/CGRP HLH-OB2 enhancer element as a probe were performed as described for the electrophoretic mobility shift assays with the exception that 9 µg of nuclear extract was used per reaction. The modified DNA contained bromodeoxyuridine and bromodeoxycytosine in place of dT and dC (Genosys Inc.) on both strands to increase UV cross-linking efficiency. Competitor oligonucleotides were preincubated for 10 min on ice with the nuclear extract before addition of the probe. The reactions were then UV irradiated for 15-45 min with a 302 nm UV light source (VWR; UVP model) at a distance of about 5 cm on ice. The reactions were resolved on a 10% SDS-polyacrylamide gel.


RESULTS

Activity of the CT/CGRP Enhancer in the Context of Flanking Sequences

We have previously shown that the CT/CGRP enhancer contains adjacent HLH and octamer binding sites (referred to as HLH-OB2, or HO, enhancer) (-1043 to -1025 bp) and that this DNA is sufficient for cell-specific enhancer activity (16). In this study, we have addressed whether the HLH-OB2 enhancer is required for enhancer activity in the context of flanking DNA. We made an insertion mutation in the HLH motif of the enhancer and measured activity in a C cell line and a non-C cell line. The mutation was tested in the context of two different 5'-flanking fragments of the CT/CGRP gene, a 1250-bp fragment that is sufficient to direct expression to thyroid C cells in transgenic mice (11) and a 205-bp fragment that contains other HLH sites implicated in control of the human CT/CGRP gene (13, 14), as well as consensus sites for SP1 and AP-2 factors. The 1250-bp region also contains elements responsive to cAMP and Ras-activated signal transduction pathways (17, 18, 20). The HLH site within the CT/CGRP HLH-OB2 enhancer was disrupted by inserting an 8-bp BamHI linker into the HLH site. Reporter genes containing either the 1250-bp CT/CGRP promoter (CT/CGRP-lacZ) linked to the beta -galactosidase gene or the 205-bp distal enhancer (1125-920-TK-luc) linked to the thymidine kinase promoter and luciferase gene were transfected into the CA77 C cell line. Mutation of the HLH site reduced the promoter activity of the 1250-bp CT/CGRP reporter gene to about 40% of the wild type promoter (Fig. 1A). Similarly, mutation of the HLH site reduced the distal enhancer activity to 20-25% of the wild type enhancer (Fig. 1A). When these constructs were transfected into the heterologous COS-7 cells, there was little to no significant change in promoter activity upon mutation of the HLH motif (Fig. 1B). Similar results were obtained when 1125-920-TK-luc was transfected into the CT/CGRP producing 44-2C cells or the non-CT/CGRP producing HeLa cells (data not shown).


Fig. 1. Activity of the CT/CGRP HLH-OB2 enhancer in the context of flanking sequences. Reporter fusion genes were transfected into CT/CGRP producing CA77 cells (A) and non-CT/CGRP producing COS cells (B). Cell extracts were assayed for luciferase or beta -galactosidase activity/20 µg of protein. Transfection efficiencies between the different plasmids were normalized by inclusion of internal cotransfection controls under control of the cytomegalovirus (CMV) promoter. CMV-luciferase was included with the CT/CGRP-beta -galactosidase plasmids and CMV-lacZ was included with the TK-luciferase plasmids. To facilitate comparison between different experiments, the means and standard errors from at least four experiments are reported relative to the mean activities of CT/CGRP-beta -gal and TK-luciferase. The striped and black boxes represent the 205-bp distal enhancer region and the 18-bp HLH-OB2 enhancer, respectively. The insertion point of the BamHI linker into the HLH-OB2 enhancer is indicated.
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In the heterologous COS cells, there was a relatively small increase in TK promoter activity mediated by the 205-bp distal enhancer. We had previously observed this increase in other heterologous cells and suggested that the distal enhancer contains both cell-specific and non-cell-specific elements (16). Since the mutant and wild type distal enhancers have essentially the same activity in COS cells, this demonstrates that the enhancement in heterologous cells is not due to a low level of HLH-OB2 enhancer activity but rather to the non-cell-specific sites. Taken together, these results indicate that the HLH-OB2 enhancer plays a major role in cell-specific CT/CGRP expression.

The CT/CGRP HB1 Complex Is a Heterodimer of USF-1 and USF-2 HLH Proteins

We then set out to identify the HLH protein that binds the HLH-OB2 enhancer. We have previously used electrophoretic mobility shift assays to characterize several factors that bind this enhancer (16). These factors include an HLH protein (HB1) and two octamer-binding proteins, the ubiquitous Oct-1 and the cell-specific OB2 protein. Weaker complexes, including some that may contain HLH proteins (16), have been detected with some nuclear extracts; however, characterization of these complexes has been hampered by inconsistent detection and variable competition results. It should be noted that a faint complex beneath Oct-1 that initially fit the criteria of a complex containing OB2 and an HLH (16) does not appear to contain OB2 upon examination of additional mutations discussed below (e.g. HOm4, HO + A). Consequently, we have focused on the major HLH complex, HB1. Identification of HB1 in the mobility shift assay is shown by competition with an excess of unlabeled DNA containing an AP4 HLH protein binding site and lack of competition by mutated HLH-OB2 DNA containing two point mutations in the HLH motif (HLHmut1) (Fig. 2A).


Fig. 2. The CT/CGRP HB1 complex is a heterodimer of USF-1 and USF-2 HLH proteins. Radiolabeled HLH-OB2 oligonucleotide probe was incubated with 44-2C nuclear extract and complexes resolved on a non-denaturing polyacrylamide gel. The positions of Oct-1, HB1, and OB2 are indicated. A, 44-2C nuclear extracts were preincubated without competitor (lanes 1 and 7) or with 50-fold molar excess of the unlabeled HLHmut1 (lane 2) or AP4 (lane 3) competitor oligonucleotides prior to incubation with the HLH-OB2 DNA probe, or incubated with three different USF polyclonal antisera after the addition of the HLH-OB2 probe (lanes 4-6, respectively). The supershift complex seen upon addition of USF-1 (C-20) antibody is indicated. In lanes 8-11, the extract was preincubated with a 5-fold molar excess of unlabeled octamer oligonucleotide, with or without antibodies, or a 50-fold molar excess of unlabeled USF oligonucleotide, as indicated. This concentration of octamer competitor was used to remove Oct-1 and allow better visualization of the supershift complex. B, HB1 is not recognized by an E12 antibody. Mobility shift assay as in A, except in this assay the samples were loaded in a glycerol buffer instead of a Ficoll buffer. The extracts were preincubated with the USF-2 (C-20) antiserum (lane 2), E12 HLH antiserum (lane 3), or CBP antiserum (lane 4).
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To identify the HLH protein within the HB1 complex, we tested several antisera against candidate proteins. Addition of a USF antibody to the mobility shift assay disrupted the HB1 complex, suggesting that HB1 is comprised of USF (Fig. 2A). Since this antibody (USF-2 C-20) recognizes both USF-1 and USF-2, we then determined which USF protein, or both, was present in HB1. To do this, we took advantage of antibodies that are specific for epitopes on USF-1 and USF-2. Addition of either antibody completely removed the HB1 complex (Fig. 2A). The USF-1 (C-20) antibody supershifted the HB1 complex, whereas addition of USF-2 (N-18) disrupted the HB1 complex. To more clearly resolve the supershifted complex from the Oct-1 complex, a small excess of consensus octamer competitor DNA was included in the binding assay (Fig. 2A, lanes 8-11). As a control, an excess of a DNA competitor containing the consensus USF binding site was also included and shown to compete the supershifted complex (Fig. 2A, lane 10). Identical results were seen in the absence of the octamer DNA competitor (data not shown). As additional controls, the effect of the USF-2 (C-20) antibody was blocked by preincubation with 0.5 µg of the C-20 peptide, and addition of preimmune sera from a different rabbit did not affect any of the complexes (data not shown). Consequently, three different polyclonal antisera raised against USF-1 and USF-2 specifically recognize HB1. Since antibodies to either protein were able to displace the entire complex, this demonstrates that the enhancer is bound by a heterodimer of USF-1 and USF-2.

We then tested antibodies against other proteins, including HLH proteins. Addition of antisera directed against the human E12 HLH protein or CREB-binding protein (CBP) did not affect the HB1 complex (Fig. 2B), even though these antisera cross-react with their rat homologs. Similarly, HB1 was not affected by addition of antisera against the ubiquitous HLH proteins Pan (rat homolog of E12/E47) and ITF-2 or the cell-specific MASH-1 HLH protein found in C cell lines (data not shown). These results demonstrate that the CT/CGRP HLH-OB2 enhancer selectively binds the USF HLH proteins. Furthermore, similar tests done on the HB1 complex from a variety of cell lines, including the human TT C cell line, showed similar results (data not shown). Consequently, we will refer to HB1 as USF.

Binding Affinity of USF for the HLH-OB2 Enhancer

The absolute and relative binding affinities of USF for the HLH-OB2 enhancer were measured by mobility shift assays. This was a pertinent question since the central dinucleotide of the HLH-OB2 HLH site (CAGCTG) differs from the USF consensus (CACGTG), and this dinucleotide has been shown by others to play a role in USF binding (34, 40, 41, 44). Scatchard plot analysis of DNA binding to USF yielded a dissociation constant of 1.6 nM (Fig. 3). This is similar to measurements of recombinant and crude USF binding to the consensus element (0.75-1.24 nM) reported by others (35, 45). To directly compare the relative binding affinities of USF for the HLH-OB2 enhancer and the consensus USF motifs, competition assays were done using oligonucleotides containing the HLH-OB2 sequence, the AP4 HLH site, which also contains the GC central dinucleotide, and the USF consensus site (Fig. 4A). There was about 3-fold greater binding to the USF consensus site than either the AP4 or HLH-OB2 motifs based on the amount of DNA competitor required for 50% competition of binding to the HLH-OB2 probe (Fig. 4B).


Fig. 3. Binding affinity of USF to the HLH-OB2 enhancer. Mobility shift assays contained duplicate lanes of 0.002, 0.01, 0.02, 0.03, 0.04, 0.08, and 0.12 pmol of HLH-OB2 DNA and a constant amount of 44-2C nuclear extract. The amounts of bound DNA and free probe from two independent experiments were measured using an InstantImager, and the means and standard deviations are plotted in a Scatchard plot as the ratio of bound over free probe versus the concentration of bound DNA.
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Fig. 4. Relative affinities of USF for the HLH-OB2, AP4, and consensus USF motifs. A, mobility shift assay using the HLH-OB2 enhancer probe with 44-2C nuclear extract. The extract was incubated without competitor (lanes 1, 7, and 13) or with increasing amounts (1-25-fold molar excess) of unlabeled AP4 oligonucleotides (lanes 2-6) or consensus USF oligonucleotides (lanes 8-12). A similar experiment was done using the HLH-OB2 self-competitor (not shown). The positions of the different complexes and free probe are indicated. B, quantitation of binding of the USF complex to the HLH-OB2 probe in the presence of the indicated competitors relative to no competitors (control). The USF data are the means and standard deviations from two independent experiments; the self and AP4 data are from single experiments.
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USF and OB2 Binding Sites Overlap

We then performed a detailed analysis of the USF binding site on the CT/CGRP HLH-OB2 enhancer in a competition binding assay using a series of mutations in the enhancer (Table I). Binding of both USF and OB2, as well as Oct-1, were monitored in this assay. Mutation of the two 5'-nucleotides of the HLH motif (HLHm1) (ACGCTG) reduced binding of USF but not OB2 or Oct-1 (Fig. 5, lane 3). Interestingly, mutation of other nucleotides within the HLH motif (HOm1, HOm2, HOm3, and HOm4) reduced both USF and OB2 binding. In contrast, Oct-1 binding was not changed by these mutations. Conversion of the central GC dinucleotide of the HLH site to create a consensus USF site (HOm2) partially reduced the binding of OB2 (Fig. 5, lane 5). The reduction was comparable to that seen with the Octm2 mutation (Fig. 5 lane 13), which we have previously shown to have greatly reduced activity (16). This result argues that the GC dinucleotide contributes to OB2 binding. In support of this conclusion, both USF and OB2 bound a DNA containing the 3'-GCTG nucleotides of the HLH motif inserted between the HLH and octamer motifs (HO + 5) (Fig. 5, lane 9); insertion of 10 bp (HO + 10) that restores only the last two 3'-nucleotides (TG) of the HLH site has reduced OB2 binding similar to the HOm2 DNA (Fig. 5, lane 10). These data demonstrate that the OB2 site overlaps the 3' four bases of the USF site. The functional consequence of both the HO + 5 and HO + 10 insertions is greatly reduced enhancer activity (16).

Table I. HLH-OB2 enhancer oligonucleotide sequences and relative binding activities

Oligonucleotides containing wild type (WT) and mutant CT/CGRP HLH-OB2 enhancer sequences, as well as AP4, USF, and octamer sequences, are shown. The boxed sequences represent the HLH and octamer motifs. Underlined nucleotides indicate differences from the HLH-OB2 enhancer. All oligonucleotides except AP4, USF, and Oct contain BamHI ends (not shown). Relative binding activities of USF and OB2 for each element are indicated.

HLH OCT Binding
OB2 USF

WT HO       GGCAGCTG           TGCAAATCCT Yes Yes
HLHm1       GCACGCTG           TGCAAATCCT Yes No
HOm1       GCCACGGT           TGCAAATCCT No No
HOm2       GGCACGTG           TGCAAATCCT Low Yes
HOm3       GGCAGCGG           TGCAAATCCT No No
HOm4       GGCAGCTA           TGCAAATCCT Yes No
HO + A       GGCAGCTG          ATGCAAATCCT No Yes
HO + 5       GGCAGCTGTGCTG      TGCAAATCCT Yes Yes
HO + 10       GGCAGCTGTGCTAGAGTG TGCAAATCCT Low Yes
HOhum       GGCAGCTG           TGCAAACGGC Yes Yes
Octm1       GGCAGCTG           CGCAAATCCT Low Yes
Octm2       GGCAGCTG           TGCAATGCCT Low Yes
AP4   AAGAACCAGCTG           TGGAAT No Yes
USFcon CACCCGGTCACGTGGCCTACA No Yes
Oct con                   GATCGAATGCAAATCACTAGCT Low No


Fig. 5. USF and OB2 recognition sites overlap. A, mobility shift assay using the CT/CGRP enhancer probe with 44-2C nuclear extract and a series of competitor DNAs. The extracts were incubated without competitor DNA (lanes 1 and 16), or preincubated with 50-fold excess wild type HLH-OB2 DNA (lane 2), mutated HLH-OB2 DNAs (lanes 3-10, 12 and 13), human HLH-OB2 DNA (lane 11), AP4 HLH DNA (lane 14), or consensus octamer DNA (lane 15). Similar results were seen with 25-fold molar excess competitors and using CA77 nuclear extract (not shown). B, schematic of the rat CT/CGRP HLH-OB2 enhancer showing the USF and OB2 binding sites determined from A. The positions of the HLH-OB2 mutations are indicated by asterisks, and the point of insertion of the +A, +5 bp, and +10-bp nucleotides are indicated by the arrow.
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Finer mutations of the USF and OB2 sites were then performed. Point mutations of the 3'-TG of the HLH site (HOm3 and HOm4) reduced USF binding, without affecting Oct-1 binding (Fig. 5, lanes 6 and 7). Mutation of the thymidine of the 3'-TG to a guanosine (HOm3) also prevented OB2 binding. However, mutation of the terminal guanosine to an adenosine (HOm4) did not affect OB2 binding. The HOm4 mutation created a consensus octamer motif. To conclusively test whether a consensus octamer motif would yield greater enhancer activity, we then inserted an adenosine residue, so as to not disrupt the HLH motif (HO + A). However, the HO + A DNA did not bind the OB2 complex (Fig. 5, lane 8). As expected, both the HOm4 and HO + A mutations bound Oct-1 better than the HLH-OB2 DNA. This confirms that OB2 does not prefer a consensus octamer motif and that the OB2 binding site extends from the octamer motif into the HLH motif.

Finally, the 3' boundary of OB2 binding was established. We had previously shown that mutation of the AT dinucleotide in the octamer motif (Octm2) reduced OB2 binding and activity (16). The human CT/CGRP HLH-OB2 enhancer differs from the rat sequence in the four most 3' nucleotides, including the thymidine mutated in Octm2. We have now shown that the human enhancer is capable of binding OB2 (Fig. 5, lane 11). It does not bind Oct-1, which underscores our previous argument that Oct-1 does not play a functional role at the HLH-OB2 enhancer (16). For comparison, additional competitors previously used to characterize the HLH-OB2 element are also shown (Fig. 5) (16). Mutation of a single residue in the 5' region of the octamer motif (Octm1) disrupts OB2 binding, the AP4 element selectively removes the USF complex, and the consensus octamer element completely removes the Oct-1 complex, and partially removes the OB2 complex. Taken together, these results suggest that the OB2 binding site overlaps with the HLH motif and that OB2 binding is fundamentally different from Oct-1.

To confirm the competition assay results we also directly tested binding and activity of the HO + A mutation. This mutation was chosen since it had the most deleterious effect on OB2 binding without affecting USF binding. Using HO + A as a probe in the mobility shift assay, the Oct-1 binding was easily observed, but OB2 binding could not be detected (Fig. 6A). This agrees with the competition assay results. The assignment of Oct-1 was confirmed by specific competition with consensus octamer DNA. In agreement with the competition studies, there was no detectable OB2 complex on the HO + A DNA. For comparison, the HLH-OB2 enhancer was used as a probe to mark the relative position and intensity of the OB2 complex.


Fig. 6. Inhibition of OB2 binding and enhancer activity by a single base insertion between the HLH and octamer motifs. A, mobility shift assay using the HO + A element as a probe with CA77 nuclear extract on a 5% gel. For reference, the far right lane contains the wild type HLH-OB2 DNA as the probe. Extract was preincubated with or without a 5-200-fold molar excess of unlabeled consensus octamer DNA competitor, as indicated. B, CA77 cells were transfected with TK-luciferase reporter genes containing the HLH-OB2 enhancer (HO) or the mutant enhancer (HO + A). Multiple experiments were compared by normalization to the HO-TK-luciferase reporter. The mean activities per 20 µg of extract and standard error of four independent experiments are shown.
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We then tested the functional consequence of the HO + A mutation. Tandem repeats of the HO + A enhancer were fused to the thymidine kinase promoter-luciferase reporter gene and transfected into CA77 C cells. The wild type HO-TK-luc reporter gave about an 8-fold increase in activity as compared with TK-luc alone, where as HO + A-TK-luc had little or no increase in activity over the parental TK-luc reporter (Fig. 6B). This indicates that the HO + A mutant enhancer has greatly reduced activity in CA77 C cells. These results are in agreement with our previous studies showing loss of activity with other mutations that affect binding (16). These results demonstrate that both the USF and OB2 binding sites are required for activity and confirm that the OB2 site functionally overlaps with the USF site.

Cross-linking of a Cell-specific Protein to the OB2 Binding Site

To characterize the factors that are contained within the OB2 complex, UV cross-linking reactions were performed using radiolabeled HLH-OB2 oligonucleotides with CA77 nuclear extract. The cross-linked products were resolved on a SDS-polyacrylamide gel and detected by autoradiography. Several bands were detected that were dependent on addition of nuclear extract and exposure to UV light (Fig. 7A). As a control, a 50-fold molar excess of unlabeled HLH-OB2 DNA was added as a competitor to determine the specificity of the cross-linking reaction (Fig. 7A, lane 4). Only the 68-kDa protein-DNA complex specifically bound the CT/CGRP HLH-OB2 enhancer. While we do not know the exact contribution of the cross-linked DNA to the 68-kDa complex size, we estimate that the minimal protein size would be about 60 kDa. This is too large to be USF (43 and 44 kDa) and too small to be Oct-1 (90 kDa). Hence it seemed likely that it could be OB2. Faint bands at the approximate sizes of USF and Oct-1 were detected using extracts from other cell lines and after competition with the HLHm1 DNA (see below), although further experiments will be needed to identify those complexes.


Fig. 7. Cross-linking of a 68-kDa protein-DNA complex to the OB2 binding site. A, radiolabeled oligonucleotide probe containing the HLH-OB2 enhancer was incubated with 9 µg of CA77 nuclear extract, UV cross-linked, and resolved on a SDS-polyacrylamide gel (lane 3). As controls, extract (lane 1) and UV light (lane 2) were omitted, and 50-fold molar excess of unlabeled HLH-OB2 DNA was included (lane 4). The apparent sizes of the molecular weight standards (prestained markers, Life Technologies Inc.) are indicated. The specific complex with an apparent size of 68 kDa is marked by an arrow. B, CA77 extracts were preincubated without a competitor (lane 1) or with 50-fold excess unlabeled wild type HLH-OB2 DNA (lane 2), the indicated mutant CT/CGRP enhancer (lanes 3 and 4), the consensus octamer DNA (lane 5), or the AP4 HLH DNA (lane 6). The 68-kDa complex is indicated by an arrow.
[View Larger Version of this Image (28K GIF file)]

To determine whether the apparent 68-kDa protein was OB2, oligonucleotides containing mutations in the HLH-OB2 enhancer or consensus HLH and octamer motifs were added as competitors in the cross-linking reactions (Fig. 7B). Both OB2 and the 68-kDa protein did not bind the HO + A mutant sequence or the AP4 HLH motif yet did bind the HLH-OB2 HLH mutant (HLHmut1) DNA and partially bound the octamer consensus sequence. These results indicate that the 68-kDa protein has the same binding properties as the OB2 complex defined in the mobility shift assays (compare Figs. 5 and 7).

Detection of HLH-OB2 Enhancer Activity and OB2 Protein in a Human C Cell Line

To test the significance of the 68-kDa OB2 protein in CT/CGRP gene expression, we asked whether the human TT C cell line has HLH-OB2 enhancer activity. As described above, other labs had reported that HLH motifs are important for human CT/CGRP enhancer activity; however, those studies did not directly test the HLH-OB2 motif. To do this, a reporter gene containing the enhancer (HO-TK-luciferase) was transfected into the TT C cell line. The HO-TK-luciferase reporter had 10-fold greater promoter activity over the parental TK-luciferase reporter (Fig. 8A). To test whether both the HLH and OB2 sites are required for activation, the HO + A-TK-luciferase and HLHm1-TK-luciferase reporters were transfected into the TT cells. These mutations greatly reduced promoter activity, demonstrating that the enhancer requires both the HLH and OB2 motifs (Fig. 8A). These data demonstrate that the rat CT/CGRP HLH-OB2 enhancer is active in a human C cell line.


Fig. 8. Cell-specific expression of the 68-kDa protein-DNA complex and USF-OB2 enhancer activity in a human C cell line. A, luciferase activity of TK-luciferase, HLH-OB2 (HO)-TK-luciferase, HO + A-TK-luciferase, and HLHm1-TK-luciferase fusion genes transfected into the TT C cell line. Transfection efficiencies were normalized by inclusion of the CMV-lacZ plasmid. Cell extracts were assayed for luciferase and beta -galactosidase activity per 20 µg of protein. To facilitate comparison between different experiments, the mean activity and standard error from three independent experiments in duplicate of TK-luciferase and HO-TK-luciferase and two independent experiments in duplicate of HO + A-TK-luciferase (indicated by the A above the HO enhancer) and HLHm1-TK-luciferase (indicated by the double asterisks above the HO enhancer) are reported relative to the mean activity of TK-luciferase. Similar results were seen in four additional experiments of TK-luciferase and HO-TK-luciferase without the CMV-lacZ normalization (not shown). B, the HLH-OB2 probe was UV cross-linked using 9 µg of nuclear extract from the human TT C cell line. Controls include omitting extract (lane 1) and UV light (lane 2). Cross-linking was done in the absence (lane 3) or presence of 50-fold excess unlabeled HLH-OB2 (lane 4), HO + A (lane 5), or HLHm1 (lane 6) DNAs. The molecular weight standards are indicated, with an arrow marking the 68-kDa complex. C, UV cross-linking using the HLH-OB2 probe with nuclear extracts from CA77 cells (lane 1), 44-2C cells (lane 2), HeLa cells (lane 3), B103 cells (lane 4), GH3 cells (lane 5), or Rat-1 cells (lane 6). The molecular weight markers are indicated, with an arrow marking the 68-kDa complex.
[View Larger Version of this Image (30K GIF file)]

We then asked whether the OB2 protein was present in the human C cell line nuclear extract. A 68-kDa protein-DNA complex was detected by cross-linking reactions, similar to that seen with the rat CA77 cells (Fig. 8B). Specific competitions confirmed that this protein had the same binding properties as the rat OB2 protein. The HLH-OB2 (self) and HLH-OB2 HLHmut1 competitors removed the human 68-kDa protein-DNA complex, whereas HO + A mutant DNA did not affect the binding of this protein (Fig. 8B). This agrees with our detection of OB2 binding in mobility shift assays using the human HLH-OB2 element (Fig. 5) and using TT nuclear extracts (data not shown). Hence, both rat and human C cell lines contain HLH-OB2 enhancer activity and the 68-kDa OB2 protein.

To further characterize the cell specificity of the 68-kDa protein-DNA complex, several CT/CGRP expressing and non-expressing cell lines were surveyed. The CT/CGRP producing CA77 and 442C nuclear extracts contains the 68-kDa protein, whereas HeLa, GH3, and Rat-1 cells, which do not express CT/CGRP, did not yield a 68-kDa cross-linking product (Fig. 8C). Since GH3 cells are a pituitary neuroendocrine cell line, this suggests that OB2 is apparently not expressed in all neuroendocrine cell types. However, we cannot rule out the possibility that OB2 is expressed at a low concentration or that it is not activated in these cells. Interestingly, the neuronal-like B103 cells do express CT and CGRP mRNAs, yet do not appear to have OB2 binding activity. This is consistent with our findings that the HLH-OB2 enhancer is not active in B103 cells in transfection studies and has little or no detectable OB2 complex in mobility shift assays (data not shown). These results suggest that OB2 is a cell-specific factor found in a subset of neuroendocrine cells.


DISCUSSION

We have found that the CT/CGRP HLH-OB2 enhancer contains overlapping motifs bound by USF HLH proteins and the cell-specific OB2 protein. The combination of OB2 with the HLH protein is required for activation of the enhancer. The relative importance of this enhancer was demonstrated by the reduced activity seen upon mutation of the USF site even in the context of flanking DNA containing other enhancer elements, including HLH sites that lack an adjacent OB2 motif. The significance of the HLH-OB2 enhancer was further underscored by its activity in a human C cell line and the presence of both USF and OB2 protein in these cells.

USF bound the CT/CGRP enhancer exclusively as a heterodimer of USF-1 and USF-2, which is consistent with reports that these proteins often dimerize with each other (34, 35). The finding that USF bound the HLH-OB2 enhancer was somewhat unexpected since USF has a fairly well established consensus binding site of CACGTG (25-27), which differs in the central dinucleotide from the CT/CGRP HLH-OB2 HLH motif of CAGCTG. This latter sequence is preferably recognized by the E12 and myoD class of HLH proteins, not the USF proteins (46). Hence, it was important to establish that USF was binding the HLH-OB2 enhancer with reasonable affinity. Our calculated dissociation constant of 1.6 nM and the finding that USF prefers the consensus site is in agreement with published observations for USF (34, 35, 40, 41, 45). It should be noted that USF binding to the CAGCTG element in vitro has been reported to be strongly influenced by magnesium concentration (44); however, we did not detect any effect of 0.1-2.5 mM MgCl2 on USF binding (data not shown). Irrespective of the in vitro data, the CAGCTG motif from the amyloid beta -protein precursor gene promoter has been shown to be bound and transactivated in vivo by USF (40), and USF has been shown to transactivate nonconsensus elements in other promoters (28, 29). These studies support the possibility that USF can recognize a nonconsensus site such as found in the CT/CGRP enhancer. The question then is why might the CT/CGRP enhancer have retained a less than optimal USF site? Based on the HOm2 mutation, we suggest that the nonconsensus USF site has been maintained to allow optimal binding of OB2.

OB2 was shown to be a single ~68-kDa DNA binding protein whose binding site extended from the octamer motif into the HLH motif. Fine mapping of the OB2 binding site strongly argues that OB2 differs from Oct-1 and that Oct-1 binding to the CT/CGRP HLH-OB2 enhancer is nonfunctional, as previously suggested (16). This is best exemplified by the HO + A mutation, which created a consensus octamer site, yet virtually eliminated OB2 binding and enhancer activity. Likewise, the human CT/CGRP HLH-OB2 enhancer binds OB2 but does not bind Oct-1. Another interesting feature of OB2 that came from these studies is its cell specificity. The B103 cerebellum cell line expresses the CT/CGRP gene, yet lacks CT/CGRP HLH-OB2 enhancer activity and OB2 protein. Consistent with this observation, CT/CGRP promoter fragments containing the HLH-OB2 enhancer can direct expression to peripheral neurons and C cells in transgenic mice but apparently not to the central nervous system (11, 12). These results suggest that different enhancer factors may control CT/CGRP gene expression in the central nervous system.

The synergistic CT/CGRP HLH-OB2 enhancer activity is an ideal target for regulation. We have previously demonstrated this point by showing that retinoic acid (42), dexamethasone (15), and a serotonergic agonist3 all can repress CT/CGRP gene expression through the CT/CGRP HLH-OB2 enhancer. The possibility that USF activity can be regulated has been suggested by Riccio et al. (32), who have shown that transforming growth factor-beta can regulate gene expression through overlapping USF-CTF/NF-I sites in the type 1 plasminogen activator inhibitor gene (32). Mutations in either the USF or CTF/NF-1 sites reduced transcriptional activation upon exposure to transforming growth factor-beta . These results suggest that synergistic interactions between USF and other factors may be a common target for transcriptional regulation.

Based on this study, we propose a model in which the CT/CGRP gene is controlled by the combinatorial action of a ubiquitous USF HLH heterodimer and the cell-specific OB2 activator. The mechanism by which USF-1 and/or USF-2 interact with OB2 to activate gene expression remains to be determined but may involve a direct protein-protein interaction between these proteins or interactions through unidentified cofactors. Using the mobility shift assay, we have been unable to unambiguously identify a complex containing both USF and OB2. Whether USF and OB2 can co-occupy the enhancer or bind in a mutually exclusive or sequential manner remains to be determined. In either case, USF and OB2 are apparently not required for each other's DNA binding activity since both proteins could bind to DNA with relatively high affinities, at least in vitro.4 While we cannot exclude the possibility that cell-specific or other HLH proteins can fulfill the HLH role in vivo, we can rule out the MASH-1 protein, since we and others have now shown that the CT/CGRP gene is expressed in mice lacking MASH-1 (47).2 There is precedence for ubiquitous HLH proteins allowing cell-specific gene expression via functional interactions with other transactivators. For example, the insulin gene has been proposed to be controlled by the combinatorial actions of E47 HLH proteins and cell-specific homeodomain proteins (22, 23). The USF HLH proteins have also been shown to functionally interact with other DNA binding proteins to activate transcription, including in a cell-specific manner (32, 33, 38). In the case of the CT/CGRP enhancer, we propose that cell specificity is provided by the OB2 protein.


FOOTNOTES

*   This work was supported by National Institutes of Health Grant R01 HD25969, with tissue culture support provided by the Diabetes and Endocrinology Center (DK25295).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
   To whom correspondence should be addressed: Dept. of Physiology and Biophysics, 51 Newton Rd., University of Iowa, Iowa City, IA 52242. Tel.: 319-335-7872; Fax: 319-335-7330; E-mail: andrewrusso{at}uiowa.edu.
1   The abbreviations used are: CT/CGRP, calcitonin/calcitonin gene-related peptide; CT, calcitonin; HLH, helix-loop-helix; OB2, octamer-binding protein 2; USF, upstream stimulatory factor; TK, thymidine kinase; CMV, cytomegalovirus; CBP, CREB-binding protein; MASH-1, mammalian achaete-scute homolog-1; HO, HLH-OB2; HB1, HLH binding protein 1; CTF/NF-I, CCAAT-binding transcription factor/nuclear factor I; DMEM, Dulbecco's modified Eagle's medium; bp, base pair(s); FBS, fetal bovine serum.
2   T. M. Lanigan and A. F. Russo, submitted for publication.
3   P. Durham and A. F. Russo, submitted for publication.
4   B. Gierasch and A. F. Russo, unpublished data.

ACKNOWLEDGEMENTS

We gratefully acknowledge Bill Gierasch, Paul Durham, Shannon DeRaad, and Lois Tverberg for their discussions and generous assistance with these studies.


REFERENCES

  1. Rosenfeld, M. G., Mermod, J.-J., Amara, S. G., Swanson, L. W., Sawchenko, P. E., Rivier, J., Vale, W. W., and Evans, R. M. (1983) Nature 304, 129-135 [Medline] [Order article via Infotrieve]
  2. McDermott, M. T., and Kidd, G. S. (1987) Endocr. Rev. 8, 377-390 [Abstract]
  3. Copp, D. H. (1992) Endocrinology 131, 1007-1008 [Medline] [Order article via Infotrieve]
  4. Brain, S. D., Williams, T. J., Tippins, J. R., Morris, H. R., and MacIntyre, I. (1985) Nature 313, 54-56 [Medline] [Order article via Infotrieve]
  5. Preibisz, J. J. (1993) Am. J. Hypertens. 6, 434-450 [Medline] [Order article via Infotrieve]
  6. Edvinsson, L., and Goadsby, P. J. (1994) Cephalagia 14, 320-327 [CrossRef][Medline] [Order article via Infotrieve]
  7. Russo, A. F., Lanigan, T. M., and Sullivan, B. E. (1992) Mol. Endocrinol. 6, 207-218 [Abstract]
  8. Clark, M. S., Lanigan, T. M., and Russo, A. F. (1995) Methods: A Companion to Methods in Enzymology 7, 253-261 [CrossRef]
  9. Russo, A. F., and Lanigan, T. M. (1996) in Genetic Mechanisms in Multiple Endocrine Neoplasia Type 2 (Nelken, B. D., ed), pp. 137-161, Landes, Austin, TX
  10. Clark, M. S., Lanigan, T. M., Page, N. M., and Russo, A. F. (1995) J. Neurosci. 15, 6167-6178 [Abstract]
  11. Stolarsky-Fredman, L., Leff, S. E., Klein, E. S., Creshaw, E. B., III, Yeakley, J., and Rosenfeld, M. G. (1990) Mol. Endocrinol. 4, 497-504 [Abstract]
  12. Baetscher, M., Schmidt, E., Shimizu, A., Leder, P., and Fishman, M. C. (1991) Oncogene 6, 1133-1138 [Medline] [Order article via Infotrieve]
  13. Peleg, S., Abruzzese, R. V., Cote, G. J., and Gagel, R. F. (1990) Mol. Endocrinol. 4, 1750-1757 [Abstract]
  14. Ball, D. W., Compton, D., Nelkin, B. D., Baylin, S. B., and deBustros, A. (1992) Nucleic Acids Res. 20, 117-123 [Abstract]
  15. Tverberg, L. A., and Russo, A. F. (1992) J. Biol. Chem. 267, 17567-17573 [Abstract/Free Full Text]
  16. Tverberg, L. A., and Russo, A. F. (1993) J. Biol. Chem. 268, 15965-15973 [Abstract/Free Full Text]
  17. Monia, Y. T., Peleg, S., and Gagel, R. F. (1995) Mol. Endocrinol. 9, 784-793 [Abstract]
  18. deBustros, A., Baylin, S. B., Levine, M. A., and Nelkin, B. D. (1986) J. Biol. Chem. 261, 8036-8041 [Abstract/Free Full Text]
  19. Watson, A., and Latchman, D. (1995) J. Biol. Chem. 270, 9655-9660 [Abstract/Free Full Text]
  20. Thiagalingam, A., deBustros, A., Borges, M., Jasti, R., Compton, D., Diamond, L., Mabry, M., Ball, D. W., Baylin, S. B., and Nelkin, B. D. (1996) Mol. Cell. Biol. 16, 5335-5345 [Abstract]
  21. Struhl, K. (1991) Neuron 7, 177-181 [Medline] [Order article via Infotrieve]
  22. German, M. S., Wang, J., Chadwick, R. B., and Rutter, W. (1992) Genes Dev. 6, 2165-2176 [Abstract]
  23. Peers, B., Leonard, J., Sharma, S., Teitelman, G., and Montminy, M. R. (1994) Mol. Endocrinol. 8, 1798-1806 [Abstract]
  24. Yoon, S. O., and Chikaraishi, D. M. (1994) J. Biol. Chem. 269, 18453-18462 [Abstract/Free Full Text]
  25. Carthew, R. W., Chodosh, L. A., and Sharp, P. A. (1985) Cell 43, 439-448 [Medline] [Order article via Infotrieve]
  26. Sawadogo, M., and Roeder, R. G. (1985) Cell 43, 165-175 [Medline] [Order article via Infotrieve]
  27. Miyamoto, N. G., Moncollin, V., Egly, J. M., and Chambon, P. (1985) EMBO J. 4, 3563-3570 [Abstract]
  28. Carthew, R. W., Chodosh, L. A., and Sharp, P. A. (1987) Genes Dev. 1, 973-980 [Abstract]
  29. Chodosh, L. A., Carthew, R. W., Morgan, J. G., Crabtree, G. R., and Sharp, P. A. (1987) Science 238, 684-688 [Medline] [Order article via Infotrieve]
  30. Scotto, K. W., Kaulen, H., and Roeder, R. G. (1989) Genes Dev. 3, 651-662 [Abstract]
  31. Potter, J. J., Cheneval, D., Dang, C. V., Resar, L. M. S., Mezey, E., and Yang, V. W. (1991) J. Biol. Chem. 266, 15457-15463 [Abstract/Free Full Text]
  32. Riccio, A., Pedone, P. V., Lund, L. R., Olesen, T., Olsen, H. S., and Andreasen, P. A. (1992) Mol. Cell. Biol. 12, 1846-1855 [Abstract]
  33. Bresnick, E. H., and Felsenfeld, G. (1993) J. Biol. Chem. 268, 18824-18834 [Abstract/Free Full Text]
  34. Pognonec, P., and Roeder, R. G. (1991) Mol. Cell. Biol. 11, 5125-5136 [Medline] [Order article via Infotrieve]
  35. Sirito, M., Lin, Q., Maity, T., and Sawadogo, M. (1994) Nucleic Acids Res. 22, 427-433 [Abstract]
  36. Morgan, J. G., Courtois, G., Fourel, G., Chodosh, L. A., Campbell, L., Evans, E., and Crabtree, G. R. (1988) Mol. Cell. Biol. 8, 2628-2637 [Medline] [Order article via Infotrieve]
  37. Meier, J. L., Luo, X., Sawadogo, M., and Straus, S. E. (1994) Mol. Cell. Biol. 14, 6896-6906 [Abstract]
  38. Navankasattusas, S., Sawadogo, M., Van Bilsen, M., Dang, C. V., and Chien, K. R. (1994) Mol. Cell. Biol. 14, 7331-7339 [Abstract]
  39. Halle, J.-P., Stelzer, G., Goppelt, A., and Meisterernst, M. (1995) J. Biol. Chem. 270, 21307-21311 [Abstract/Free Full Text]
  40. Kovacs, D. M., Wasco, W., Witherby, J., Felsenstein, K. M., Brunel, F., Roeder, R. G., and Tanzi, R. E. (1995) Hum. Mol. Genet. 4, 1527-1533 [Abstract]
  41. Vostrov, A. A., Quitschke, W. W., Vidal, F., Schwarzman, A. L., and Goldgaber, D. (1995) Nucleic Acids Res. 23, 2734-2741 [Abstract]
  42. Lanigan, T. L., Tverberg, L. A., and Russo, A. F. (1993) Mol. Cell. Biol. 13, 6079-6088 [Abstract]
  43. Vierra, C. A., Jacobs, Y., Ly, L., and Nelson, C. (1994) Mol. Endocrinol. 8, 197-209 [Abstract]
  44. Bendall, A., and Molloy, P. M. (1994) Nucleic Acids Res. 22, 2801-2810 [Abstract]
  45. Sawadogo, M. (1988) J. Biol. Chem. 263, 11994-12001 [Abstract/Free Full Text]
  46. Dang, C. V., Dolde, C., Gillison, M. L., and Kato, G. J. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 599-602 [Abstract]
  47. Blaugrund, E., Pham, T. D., Tennyson, V. M., Lo, L., Sommer, L., Anderson, D. J., and Gershon, M. D. (1996) Development 122, 309-320 [Abstract/Free Full Text]

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