(Received for publication, February 3, 1997, and in revised form, March 5, 1997)
From Cornell University, Division of Nutritional Sciences, Savage
Hall, Ithaca, New York 14853-6301, and the Institut
de Génétique et de Biologie Moléculaire et
Cellulaire, BP 163, F-67404 Illkirch Cedex, France
The retinoid X receptor (RXR) regulates target gene transcription via its association with cognate DNA response elements either as a homodimer or as a heterodimer with a number of other nuclear receptors. We previously demonstrated that, in solution, RXR forms tetramers with a high affinity and that ligand binding leads to dissociation of receptor tetramers to smaller species. Here it is shown that RXR tetramers form stable complexes with direct repeats (DR-1 or DR-5) or palindromic (TREpal) response elements. Binding of RXR tetramers to cognate DNA occurs with a significantly higher affinity as compared with dimers. Ligand binding by DNA-bound RXR tetramers results in their dissociation to DNA-bound dimers, a process that is completely reversed upon removal of the ligand. Formation of stable tetramer-DNA complexes requires binding of two oligonucleotides/tetramer. It is proposed that ligand-dependent modulation of the oligomeric state of RXR is a regulatory feature of this nuclear receptor.
The retinoid receptors, retinoid X receptors (RXRs)1 and retinoic acid receptors (RARs), are ligand-activated transcriptional regulators that belong to a superfamily of nuclear receptors that mediate the effects of small lipophilic ligands on gene transcription. RARs can bind and are activated by the vitamin A metabolite all-trans-retinoic acid, while both RXRs and RARs can bind and are activated by the 9-cis isomer of retinoic acid (9cRA) (1, 2) although the physiological role of this ligand is not clear at present (3, 4). Besides RXR and RAR, the hormone nuclear receptor superfamily also includes the steroid receptor, thyroid hormone receptors (TR), vitamin D receptor (VDR), and peroxisome proliferator-activated receptor as well as a number of orphan receptors (for reviews, see Refs. 5-8). These proteins modulate transcription via their association with specific nucleotide sequences, termed response elements (REs), that are located in the promoter region of target genes. Recognition sequences for thyroid and retinoid receptors consist of everted, inverted, or direct repeats of the consensus motif RG(G/T)TCA (9-11).
The association of some receptors with cognate DNA and activation of their target genes requires the presence of only a single, albeit extended, half-site of a RE. However, DNA binding by most hormone nuclear receptors relies on formation of receptor dimers and requires the presence of two repeats of the consensus core motif with a variable spacing between them (12-15). Self-association of RXR, RAR, TR, and VDR results in the formation of homodimers that can bind to cognate DNA (16-21). However, heterodimerization of these receptors with RXR usually increases the affinity and specificity of their association of with DNA (22-26). This suggests that RXR can function as a general binding partner that communicates between converging signaling pathways (27-29). Indeed, it has been recently shown that RXR is a transcriptionally active partner in RXR·RAR heterodimers and can contribute to gene activation in vivo resulting in cell differentiation and apoptosis (30).
It is well documented that ligands are important for transcriptional regulation by retinoid receptors, but only recently have the exact functions of ligand binding for the activities of the receptors begun to become clear. It has been demonstrated that ligand binding by RAR and TR results in the release of factors that associate with the unliganded receptor and act as co-repressors (31-35). The association of these co-repressors with RXR is much weaker and is not affected by ligand, suggesting that ligand-dependent regulation of the activity of RXR relies on a different mechanism (32, 34, 35). Several co-activators, transcriptional intermediary factors, and general transcription factors have been recently shown to associate with RXR in a ligand-dependent or ligand-enhanced fashion (36-42). It was suggested that ligand binding induces nuclear receptors to undergo transconformations that generate surfaces for binding of the accessory factors (43-46).
Another important clue regarding the mechanism by which the activity of RXR may be regulated by its ligand emerged from our recent findings that RXR forms tetramers in solution with a high affinity and that the oligomeric state of this receptor is modulated by its ligand (47, 48). A regulatory role for this phenomenon was suggested by the observations that ligand binding by RXR tetramers displays a pronounced positive cooperativity and leads to a rapid and massive dissociation of receptor tetramers to dimers and monomers (48, 49). It was thus proposed that ligand-induced dissociation of RXR tetramers is an essential step in transcription activation mediated by this receptor. In the present work we set out to investigate the consequences of the self-association of RXR for the DNA binding pattern by the receptor.
Recombinant RXR lacking the N-terminal A/B
domain (RXR
AB) was obtained by overexpression in E. coli and isolated as described previously (47, 50). Isolated
protein was found to possess 0.65-0.85 mol of ligand binding sites/mol
of protein. The receptor showed the expected ligand selectivity as it
tightly bound 9cRA and several RXR-selective ligands but not
all-trans-retinoic acid (51). The receptor also displayed
the expected specificity for consensus response elements; it bound
with a significantly stronger affinity to a DR-1 RE as compared with a
DR-5 or TREpal REs. Purified protein was dialyzed against a
buffer containing 10 mM Hepes, pH 8.0, 0.1 mM
EDTA, 0.4 mM dithiothreitol, 400 mM KCl, and
5% glycerol, and was stored at
20 °C in 50% glycerol. Protein
concentration was determined with the Bradford Assay (Bio-Rad) using
bovine serum albumin as a standard.
Bacterial extracts containing full-length hRXR were prepared as
described by Lupisella et al. (52).
The following oligonucleotides containing
the following response elements were used: DR-1, a response element
containing two direct repeats spaced by a single nucleotide
(5-TCGAGGGTAGGGGTCAGAGGTCACTCGTCGA-3
); DR-5, a response element composed of two direct repeats spaced by 5 nucleotides
(5
-AGCTTGGCGCCGGGTCACCGAAAGGTCAGAATT-3
);
TREpal (5
-AGCTTGGCGCCTCAGGTCATGACCTGAGAATT-3
); half-site,
5
-AGCTTGGCGCCAGGGGTCAGGTCAGAATT-3
; DR-1ext
(5
-TCGACGGCAACAGCGGACATTTAAGTCGGTTAAGGGCTAGGGGTCAGAGGTCACTCCATA ATTGTCACACCGGATAAGGAGATTTGTGCTCGA-3
).
The oligonucleotides were synthesized and purified at the Cornell DNA synthesis facility. Single-stranded DNA was annealed (53), and double-stranded DNA isolated on Centrex centrifugal filter units. Oligonucleotides were end-labeled with [32P]dCTP by filling in with Klenow fragments, and free nucleotides were removed with the Qiagen nucleotide removal kit.
Electrophoretic Mobility Shift Assays (EMSAs)1 µl of
labeled oligonucleotide (50-200 nM; final concentration,
2.5-10 nM) and 1 µl of 2.4 mg/ml dI-dC were mixed with
the indicated amounts of RXRAB in 18 µl of 10 mM
Hepes, pH 8.0, 0.1 mM EDTA, 0.4 mM
dithiothreitol; 100 mM KCl, and 15% glycerol. Ligands were
added from a concentrated solution in ethanol or Me2SO to a
final concentration of 1 µM, and mixtures were incubated for 15 min at room temperature. Protein-DNA complexes were resolved by
electrophoresis on 5% polyacrylamide gels (0.5 × TBE, 2-3 h prerun at 100 V, 2 h run at 25 mA/gel), the gel was dried, and protein-DNA complexes were visualized by autoradiography. During electrophoresis, the gel was cooled with circulating water at 12 °C.
Binding of RXR to DR-1 RE was analyzed by EMSAs using polyacrylamide gels with various acrylamide concentrations. The mobilities of receptor-DNA complexes were analyzed by the method of Ferguson as described by Bollag and Edelstein (54). The retardation factors (Rf values) of individual bands in gels of differing acrylamide concentrations were plotted versus the acrylamide concentration, and the resulting slopes were compared with those of protein standards with known molecular masses. These standards were ovalbumin (45 kDa), bovine serum albumin (67 kDa; monomer, dimer, and trimer), transferrin (76 kDa), transthyretin (55 kDa), and chymotrypsin (26 kDa).
Photobleaching of Holo-RXRRXRAB (10 µM
in buffer A) was complexed with 9cRA at a ligand:protein molar ratio of
5:1. A small aliquot was removed and kept in the dark at 4 °C. The
remainder of holo-RXR was photobleached using a hand-held UV lamp (4 watts, 366 nm). The lamp was placed about 1 inch above a quartz cuvette
on ice containing the holo-RXR solution for a total of 6 min.
We previously showed that RXR exists in
solution as a monomer, dimer, and tetramer and that tetramers become
the predominant species of the receptors at concentrations on the order
of 50-100 nM (47). As DNA binding by RXR is essential for
its activity, it was of interest to explore which of the receptor
oligomers can bind to cognate DNA. The DNA binding pattern of RXR was
analyzed by EMSAs in the presence of a DR-1 RE (Fig. 1,
lane 1), a DR-5 RE (lane 3), or a
TREpal RE (lane 2). As previously reported (11), DR-5 and TREpal displayed a lower affinity for RXR as
compared with DR-1 and required higher protein concentrations for
visualization of RXR·DNA complexes. Nevertheless, two distinct
RXR·DNA complexes, with the slower moving band being the more
pronounced complex, could be observed with all three REs. Thus, RXR
appears to have two modes of association with DNA containing two
half-sites of its recognition sequence. The presence of two RXR·DR-1
complexes in EMSAs carried out with receptor obtained by expression in
Escherichia coli COS-1 cells and insect Sf9 cells was
previously noted (11, 50), but their compositions were not
explored.
To examine whether the faster moving band might represent monomeric DNA binding by RXR, EMSA was performed using an oligonucleotide containing a single half-site (Fig. 1, lane 4). No specific complex between RXR and oligonucleotides containing a half-site could be observed even at very high RXR concentrations ([RXR] = 10 µM). These data are in agreement with the reported cooperativity of DNA binding by this receptor (55) and indicate that RXR monomers have a very low affinity for the response element. Thus, the faster moving band apparent in Fig. 1 reflects a receptor-DNA complex of a higher order than monomer.
The compositions of the two DNA·RXR complexes were determined by
analyzing EMSAs using the method of Ferguson, a procedure that allows
for determining the molecular weight of species resolved by
electrophoresis under nondenaturing conditions (54). RXR/DNA mixtures
were resolved on polyacrylamide gels of varying acrylamide concentrations. The Rf values of the two RXR/DNA
bands in gels of differing acrylamide concentrations were plotted
versus the acrylamide concentration, and the resulting
slopes were compared with those of protein standards with known
molecular masses. Fig. 2 shows the Ferguson analysis
relating the negative value of the slopes to the molecular weights of
the standards. The mobilities of the two RXR·DNA complexes
(arrows in Fig. 2) corresponded to molecular masses of
110 ± 14 and 181 ± 5 kDa (mean ± S.E.M., n = 3). As the molecular masses of RXRAB and the DR-1
oligonucleotide are 40,270 and 18,200 Da, respectively, the molecular
masses of the species represented by the faster moving band is most
consistent with a complex comprised of a DNA-bound RXR dimer, while the
slower moving band most likely represents a DNA-bound tetramer. Hence, these data demonstrate that RXR can bind to DNA containing a single response element either as a dimer or as a tetramer.
RXR Tetramers Associate with DR-1 RE at an Oligonucleotide:Tetramer Ratio of 2:1
The data in Fig. 2 identified the oligomeric state of the two DNA·RXR complexes observed in EMSAs. However, these data are not precise enough to ascertain whether DNA-bound RXR tetramers are associated with 1 or with 2 oligonucleotides/tetramer, i.e. whether each dimer within the tetramer can separately interact with a response element or whether only two of the tetramer subunits are involved in DNA-receptor interactions.
To distinguish between these two possibilities, EMSAs were carried out
using a mixture of a short (31-base pair) and an extended (93-base
pair) oligonucleotide containing a DR-1 response element. It was
reasoned that if RXR tetramers associate with two DR-1 REs
simultaneously, a complex in which a tetramer is bound to one short and
one extended oligonucleotide will form when both oligonucleotides are
present. Such a complex is expected to display a mobility that is
intermediate between tetramer-DNA complexes observed in the presence of
each of the DR-1 REs separately. In the presence of the extended DR-1
(Fig. 3, lanes 1 and 2), a
predominant band with a low mobility and a minor band of higher
mobility were observed. The addition of 9cRA to mixtures of RXR with
the extended oligonucleotide led to a significant decrease in the
concentration of the slower moving band with a concomitant enhancement
of the population of the faster moving band (Fig. 3, lanes 3 and 4). We previously reported that ligand binding by RXR
tetramers in solution leads to rapid dissociation of receptor tetramers
to dimers and monomers (48, 49). The observations in lanes 3 and 4 thus indicate that, similar to the interactions of RXR
with the short DR-1 (Fig. 1), RXR binds to the extended DR-1 as a dimer and as a tetramer, reflected by the faster and the slower moving bands,
respectively. The data further imply that ligand binding by DNA-bound
RXR tetramers, similar to the behavior of tetramers in solution,
leads to their rapid dissociation to smaller species (see below). RXR
tetramers and dimers complexed with the short oligonucleotide displayed
a higher mobility than the corresponding species bound to the extended
oligonucleotide (Fig. 3, lane 9). In the presence of both
oligonucleotides, a band with an intermediate mobility between the
bands of RXR tetramers bound to the short versus the
extended oligonucleotide became visible (Fig. 3, lanes 5-8). This intermediate band most likely reflects the presence of
a complex of RXR tetramers associated with one short and one extended
DR-1 oligonucleotide, i.e. a complex that is composed of RXR
tetramers associated with two oligonucleotides. No additional bands
that might correspond to tetramers bound to a single DR-1 were
apparent.
The data thus suggest that the association of tetramers with a single oligonucleotide is weak and that binding of a second oligonucleotide significantly stabilizes the complex. Thus, binding of DR-1 oligonucleotides to RXR tetramers proceeds in a cooperative fashion. In addition, as free dimers efficiently associate with DR-1 REs, the low binding affinity toward the RE of each of the individual dimers within a RXR tetramer suggest that these dimers adopt a somewhat different conformation as compared with free dimers.
To investigate the relative affinity of RXR tetramers versus
dimers for a DR-1 RE, EMSAs of RXR with DR-1 were carried out at
increasing protein concentrations under conditions of excess protein or
excess cognate DNA (Fig. 4). Under conditions of
limiting DNA (Fig. 4A) and at a low protein concentration,
the predominant band corresponded to a tetrameric RXR·DNA complex
(Fig. 4A, lane 1). As the protein concentration
was incrementally raised, the tetrameric complex was enhanced until it
reached a peak at a protein concentration of about 50 nM
(lane 3) and then gradually diminished (Fig. 4A,
lanes 4-6). Concomitantly with the diminishment of the tetrameric complex, the dimeric complex gradually became the prominent species. In contrast, in the presence of excess DR-1, both the dimeric
and the tetrameric RXR·DNA complexes intensified monotonously as the
protein concentration was raised (Fig. 4B).
The origin of the apparent concentration dependence of DNA binding by RXR under conditions of a limiting DNA concentration is not completely clear to us at the present time. This behavior is especially puzzling if the concentration dependence of the oligomeric state of RXR in solution is taken into account; at low protein concentrations, where the concentrations of tetramers are low (47), the tetrameric species seems to be the predominant DNA-bound RXR. On the other hand, at high protein concentrations, where tetramers are the predominant species in solution (47), RXR·DNA complexes appear to be mainly dimeric. Thus, the apparent concentration dependence of protein-DNA complexes in Fig. 4A cannot be explained based on the thermodynamic properties of the system, suggesting that under some conditions EMSAs do not properly report on the equilibrium situation. It is possible that kinetic effects, such as differential instabilities of complexes during electrophoresis, play a role in bringing about the observed profiles. In support of this explanation, an examination of the bottom region of the gel shows that, in contrast to what could be expected, the amount of free DNA and the region across which free DNA diffuses increased continuously as the protein concentration was raised. This behavior is indicative of gradual dissociation of complexes during electrophoresis, which became more pronounced at higher protein concentrations. The origin of the instability of protein-DNA complexes at high protein concentration is not clear. Nevertheless, these observations demonstrate that under different conditions EMSAs can result in the appearance of either the tetrameric or the dimeric complex or in the appearance of both RXR·DNA complexes. Hence, unless a range of conditions is explored, the presence of more than one species might be overlooked.
The dissociation of protein-DNA complexes during electrophoresis in Fig. 4A, lane 1, appears to be minimal. The relative distribution of tetrameric and dimeric RXR·DNA complexes in this lane may thus be used to assess the relative binding affinities of receptor dimers and tetramer for DR-1 RE. The concentration of RXR used in lane 1 was 12.5 nM, a concentration in which the fraction of RXR tetramers in solution is significantly lower than the fractions of either monomers or dimers (47). Nevertheless, the tetrameric RXR·DNA complex predominated, indicating that the binding energy driving the formation of the tetrameric RXR-DR-1 complex is significantly stronger as compared with that of the dimeric RXR-DR-1 complex.
Overall, the observations in Figs. 3 and 4 indicate that both RXR tetramers and dimers can bind to oligonucleotides containing two half-sites of their RE and that formation of a stable RXR tetramer-DNA complex requires the association of each dimer within the tetramers with a separate RE. The data also show that the affinity of REs for tetramers is higher than the affinity toward dimers.
Ligand Binding Induces Dissociation of DNA-bound RXR Tetramers to DNA-bound RXR Dimers in a Reversible FashionIt was previously
demonstrated that RXR tetramers, the predominant species of the
unliganded receptor in solution, dissociate into dimers and monomers
upon binding of ligand (48, 49). The observations in this paper that
RXR tetramers can bind to cognate DNA raise the question of whether
DNA-bound RXR tetramers respond to their ligand in the same vein. Fig.
5 clearly demonstrates that binding of 9cRA by DNA-bound
RXR leads to a significant decrease in the fraction of RXR bound as a
tetramer concomitantly with an increase in the fraction of RXR bound as
a dimer. The dimeric RXR·DNA complexes were enhanced upon the
addition of ligand over the entire range of protein concentrations used
in the experiment depicted in Fig. 4. The same effect was observed
using a variety of RXR-selective ligands (data not shown), indicating
that tetramer dissociation is a general response of this receptor to
cognate ligands. These observations demonstrate that, similar to the
behavior of the protein in solution, ligand binding by DNA-bound RXR
tetramers leads to their dissociation to DNA-bound dimers.
We previously suggested that ligand-induced dissociation of RXR tetramers might serve to regulate the activity of the receptor by varying the ratio between tetrameric and dimeric species. An important feature of such a regulatory mechanism would be the ability to "switch off," i.e. it has to be demonstrated that RXR tetramers not only dissociate upon ligand binding, but that they retain the ability to reversibly reassociate to form tetramers following removal of the ligand.
EMSA of apo-RXR with a DR-1 RE showed the expected presence of
tetrameric and dimeric protein-DNA complexes (Fig.
6A, lane 1). The addition of 9cRA
resulted in the dissociation of the tetrameric complex with a
concomitant enhancement of the dimeric complex (Fig. 6A,
lane 2). Holo-RXR was then depleted of its ligand by photobleaching. The protein was exposed to 2-min bouts of UV light for
a total of 8 min, resulting in degradation of 9cRA, which could be
followed by the decrease of the absorbance of the ligand (Fig.
6B). If ligand-induced tetramer dissociation is a reversible process, depletion of RXR of its ligand would result in reassociation of the protein to tetramers. Indeed, apo-RXR generated following photodegradation of 9cRA reassociated to form tetramers, which is
reflected by the reappearance of tetrameric RXR-DNA complex (Fig. 6,
lane 3). The similarity of the relative distribution of the
two RXR·DNA complexes prior to the addition and following the
depletion of ligand (compare lanes 1 and 3)
indicates that the ligand-induced dissociation of DNA-bound tetramers
was fully reversed upon removal of the ligand. These data provide
additional support to our hypothesis that variations in the local
concentrations of the ligand for RXR may serve as an on/off switch that
regulates activation/deactivation cycles of the receptor by modulating
its oligomeric state.
The A/B Domain of RXR
Our studies of RXR, most importantly the
characterization of the physicochemical properties of receptor
oligomers (47-49), relied on usage of a truncated protein lacking the
A/B domain (RXRAB). An important question that has remained
unanswered is whether the A/B domain might influence the
self-association of the receptor or the response of protein oligomers
to ligand binding. Due to technical difficulties in obtaining pure
full-length receptor, the assays described below were carried out using
crude extracts of E. coli expressing full-length RXR
.
EMSAs of mixtures of the bacterial extract with DR-1 RE gave rise to
two bands (Fig. 7, lanes 1-3). As expected,
the mobilities of these bands were slower than the mobilities of the
two bands observed when the truncated protein was used (data not
shown). The presence of RXR within the two complexes was confirmed by
supershifting using antibodies against RXR (Fig. 7, lanes
7-9). The addition of 9cRA led to a significant decrease in the
population of the slower moving band with a concomitant enhancement of
the faster moving band (Fig. 7, lanes 4-6). Thus,
full-length RXR
, similarly to RXR
AB, forms both dimeric and
tetrameric complexes with DR-1 and responds to its ligand by
dissociation of the DNA-bound tetramers to DNA-bound dimers. These
results demonstrate that deletion of the A/B domain has little effect
on the characteristics of self-association of RXR
. The data also
indicate that the truncated receptor RXR
AB is a valid model for
studying the self-association of RXR and the factors that regulate this
process.
Previous studies of the self-association of RXR in solution revealed that this receptor forms tetramers with a high affinity and in a cooperative fashion and that tetramers become the predominant species at concentrations in the range of 50-100 nM. It was further demonstrated that ligand binding by RXR leads to rapid dissociation of tetramers to monomers and dimers (47-49). Here it is shown that tetramers can form stable complexes with REs consisting of two direct or inverted repeats of the consensus half-site. Analyses of tetramer-DNA complexes indicated that they are formed with a significantly higher affinity as compared with dimeric DNA complexes. Tetrameric DNA complexes were found to contain two REs/tetramer and did not reveal the presence of a species consisting of a tetramer and a single RE. These observations suggest that the association of each dimer within the tetramer with a separate RE is necessary to stabilize the interaction of tetramers with cognate DNA and thus that binding of the two REs to receptor tetramers proceeds cooperatively. As free dimers associate with REs with a high affinity, the observation that association of a RE with each of the dimers within RXR tetramers depends on DNA binding by the other leads to the conclusion that dimers within RXR tetramers adopt a somewhat different conformation from that of free dimers. The observed cooperativity in association of REs with tetramers also indicates that individual subunits within tetramers communicate between themselves. This last point is in agreement with our previous observation that ligand-induced dissociation of RXR tetramers proceeds cooperatively (49), pointing at the existence of efficient communication between individual subunits of RXR tetramers.
These observations raise the question of whether RXR tetramers might be able to associate with cognate DNA in vivo. The requirement for binding of two REs for the formation of a stable tetramer-DNA complex leads one to speculate that perhaps in special promoter contexts, DNA binding by RXR tetramers allows the receptor to serve as a bridging factor between two distant regulatory elements on the chromatin. The existence of regulatory sequences far upstream from the transcriptional start site is common to many eukaryotic promoters, and it has been suggested that such distant regulatory elements could be brought close to the start site via a DNA loop mediated through protein-protein interactions (56). One example that might be relevant in this context is that of two retinoic acid REs separated by 30 nucleotides that have recently been identified in the promoter region of the retinol-binding protein gene. The two regions were found to be present on the same side of the DNA helix, and occupancy of both by retinoid receptors was shown to be necessary for transactivation. It has been proposed that protein-protein interactions between receptors bound at the two regions might play a role in regulating the transcription of this gene (57). An intriguing possibility is that DNA binding by RXR tetramers might be involved in regulating the expression of genes that contain such a complex RE within their promoter regions.
It was previously shown that four RXR molecules can cooperatively bind to the RARE of the CRBPI gene, a RE that consists of 4 or 5 half-sites separated by a single base pair (58). It is not clear, however, whether the mode by which multiple RXR subunits associate with REs containing multiple adjacent repeats is similar to the mode by which RXR tetramers associate with REs composed of only two half-sites as reported in the present work. While the tetrameric RXR·DNA complexes formed with a single RE reflect binding of DNA to preformed tetramers, the association of four RXR subunits with the CRBPI RE might simply reflect association of two separate receptor dimers with the complex RE.
The data in the present work demonstrate that, similarly to the effect of ligand on the oligomeric state of RXR in solution, binding of ligand to DNA-bound RXR tetramers induces their dissociation and leads to a concomitant increase in the population of DNA-bound receptor dimers (Fig. 5). In addition, the ligand-induced tetramer dissociation was found to be fully reversible, such that, upon removal of the ligand, the receptor reassociated and efficiently formed tetrameric RXR-DNA complexes (Fig. 6). Ligand-induced increases in the population of RXR dimers, and in DNA binding by these dimers, has been previously reported (20, 59). To account for these findings, it was suggested that ligand binding by RXR enhances the affinity of receptor monomers for each other. In conflict with this hypothesis, measurements of the equilibrium dissociation constants governing dimer formation by RXR showed that they are very similar in the absence and in the presence of ligand (48). The data in the present work, in agreement with the previous reports (20, 59), show that the population of the DNA-bound RXR dimers is indeed significantly increased upon binding of 9cRA but indicate that this response stems from ligand-induced weakening of receptor tetramers and not, as previously suggested, from changes in the affinity of receptor monomers for each other.
The observations reported here further support our postulate that binding of ligand to RXR may regulate the activity of the receptor by modulating the relative distribution of receptor monomers, dimers, and tetramers (47-49). According to this model, the bulk of apo-RXR in cells is sequestered in the form of tetramers that serve as a transcriptionally inactive reservoir for the receptor. Changes in the distribution of receptor species occur upon increases in the cellular level of 9cRA to a concentration that is sufficient for efficient binding to the receptor (see Ref. 49 for discussion of the cooperative nature of the association of 9cRA with RXR). Ligand binding leads to dissociation of receptor tetramers into dimers that can activate the homodimeric pathway of RXR and into monomers that can then interact with partner receptors and participate in heterodimeric pathways.
Similar to the present observations that unliganded RXR is bound to DR-1 as a tetramer while the liganded receptor associates with the RE as a dimer, it has been reported that unliganded VDR binds to a DR-3 RE mainly as a dimer but dissociates into monomers in the presence of 1,25(OH)2-vitamin D3 (21). It was also shown that TR binds to cognate DNA as a monomer and a dimer in the absence of ligand but binds exclusively as a monomer in the presence of T3 (19, 60, 61). These findings, taken together, suggest that modulation of the oligomeric state of hormone nuclear receptors might be a general mode of response of these proteins to their ligands. If receptor homo-oligomerization serves to sequester active subunits into an inactive reservoir as suggested above, then the question arises of why unliganded RXR self-associates to homotetramers while VDR and TR only form homodimers. An important distinction between RXR versus VDR and TR is that while RXR homodimers are transcriptionally active, homodimers of both VDR and TR are poor transcriptional activators, since these receptors seem to function predominantly via heterodimers with RXR (5, 8). It hence might be speculated that formation of homodimers by TR and VDR is sufficient to render these receptors relatively inactive. In contrast, inactivation of RXR requires sequestration of both monomers and dimers, a need that can be fulfilled by the formation of homo-oligomers of a higher order, i.e. tetramers.
In summary, the data reported here demonstrate that RXR tetramers can bind to cognate DNA containing a single response element with high affinity and that formation of RXR tetramer-DNA complexes requires a binding stoichiometry of two REs/tetramer. The data further show that ligand binding reversibly induces dissociation of DNA-bound receptor tetramers concomitantly with enhancement of the population of DNA-bound RXR dimers. Additional studies will be required to delineate the implications of this mode of DNA binding by RXR for the in vivo function of the receptor.
We thank Peter Reczek for providing bacterial
extracts containing full-length RXR and Hoffman La Roche for the
gift of 9cRA.