The SH3 Domain of Amphiphysin Binds the Proline-rich Domain of Dynamin at a Single Site That Defines a New SH3 Binding Consensus Sequence*

(Received for publication, December 23, 1996, and in revised form, March 13, 1997)

Detlev Grabs Dagger §, Vladimir I. Slepnev Dagger , Zhou Songyang , Carol David Dagger par , Mary Lynch , Lewis C. Cantley and Pietro De Camilli Dagger Dagger Dagger

From the Dagger  Department of Cell Biology and Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center for Molecular Medicine, New Haven, Connecticut 06510 and the  Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES


ABSTRACT

Amphiphysin is an SH3 domain-containing neuronal protein that is highly concentrated in nerve terminals where it interacts via its SH3 domain with dynamin I, a GTPase implicated in synaptic vesicle endocytosis. We show here that the SH3 domain of amphiphysin, but not a mutant SH3 domain, bound with high affinity to a single site in the long proline-rich region of human dynamin I, that this site was distinct from the binding sites for other SH3 domains, and that the mutation of two adjacent amino acids in dynamin I was sufficient to abolish binding. The dynamin I sequence critically required for amphiphysin binding (PSRPNR) fits in the novel SH3 binding consensus identified for the SH3 domain of amphiphysin via a combinatorial peptide library approach: PXRPXR(H)R(H). Our data demonstrate that the long proline-rich stretch present in dynamin I contained multiple SH3 domain binding sites that recognize interacting proteins with high specificity.


INTRODUCTION

Dynamin I is a neuronal GTPase concentrated in nerve terminals that plays an essential role in synaptic vesicle endocytosis and recycling (for reviews, see Refs. 1 and 2). A temperature-sensitive mutation in the dynamin I gene of Drosophila leads to rapid and massive block of synaptic vesicle endocytosis resulting in a paralytic phenotype (3-5). Ultrastructural studies have shown that dynamin I forms rings at the neck of clathrin-coated pits, and it has been hypothesized that a conformational change of the ring that correlates with GTP hydrolysis represents a key step leading to vesicle fission from the plasmalemma (6, 7). In addition, dynamin I has also been implicated in rapid endocytosis, a form of Ca2+-triggered endocytosis detectable by capacitance measurement in neuroendocrine cells (8). Dynamin isoforms (dynamin II and dynamin III) are expressed in non-neuronal cells (9-11) where they are thought to play a general role in clathrin-mediated endocytosis (12, 13).

The COOH-terminal region of dynamin I contains a 100-amino acid-long proline-rich domain (14), which undergoes regulation by protein phosphorylation and binds a variety of SH3 domains (13, 15-18). An abundant SH3 domain-containing protein, which is a major binding partner for dynamin I in nerve terminals (19), is amphiphysin, the dominant autoantigen in paraneoplastic stiff-man syndrome (20-22). Amphiphysin is closely colocalized with dynamin I at synapses where, in addition to dynamin I, it binds the presynaptic inositol-5-phosphatase synaptojanin (23). Amphiphysin binds the plasmalemmal clathrin adaptor AP2 via a region distinct from its SH3 domain (19, 24), further supporting an involvement of amphiphysin in endocytosis. In addition, amphiphysin contains regions of similarity to two yeast proteins, Rvs167 and Rvs161 (20, 25, 26), which genetic studies have shown to be implicated both in endocytosis and in the function of the actin cytoskeleton (26, 27).

As a premise to further elucidate the functional interconnections between amphiphysin and dynamin I, we investigated regions that are crucial for reciprocal interactions in the two proteins. We report that the SH3 domain of amphiphysin bound with high affinity to a short amino acid stretch in dynamin I. The amino acid sequence of the binding site fits the novel consensus for the SH3 domain of amphiphysin, which we have identified by a combinatorial peptide library.


EXPERIMENTAL PROCEDURES

Antibodies

Affinity-purified rabbit antibodies directed against amphiphysin (CD5) were generated in our laboratory (19). Anti-dynamin and anti-Grb2 monoclonal antibodies were purchased from Transduction Laboratories. Anti-cortactin (4F11) and anti-PLC1-gamma (F-7-2) monoclonal antibodies were the kind gifts of Dr. T. Parson (University of Virginia) and Dr. S. G. Rhee (National Institutes of Health), respectively.

Recombinant Proteins

Full-length polyhistidine-tagged human amphiphysin and a GST-fusion protein containing its wild type SH3 domain (construct V (amino acids 545-695) of Ref. 20) were generated as described previously (19, 20). A mutant amphiphysin SH3 domain was constructed as follows. A primer matching the 5' end (see Ref. 20) of the wild type SH3 domain fragment and a primer partially matching its 3' end (5'-ACG ATG AAT TCA ATC TAA GCG TCG GGT GAA GTT CTC TAG AAA GAG GCG TTT GTA GGT GGC-3'), which included two base changes resulting in the substitution of two amino acids at position 684 (glycine to arginine) and 687 (proline to leucine), were used in polymerase chain reactions using the wild type SH3 domain as the template. The resulting DNA fragment was cloned into pGEX2T and expressed using the Escherichia coli strain BL21 as a GST-fusion protein.

A polyhistidine human dynamin deletion construct missing the entire proline-rich domain (amino acid 1750) was expressed in the pET23c vector (Novagen). GST and GST-fusion proteins containing either the full-length proline-rich domain (PRD) or the COOH-terminal truncated forms of this domain (see Fig. 2A) were expressed in pGEX2T. The construct harboring point mutations at positions 835 (arginine to aspartic acid) and 836 (proline to alanine) (Fig. 3, construct 5) was obtained by polymerase chain reaction amplification using mutant primers. All dynamin constructs were partial sequences of the human dynamin isoform Iaa (11). Whereas the NH2-terminal primer for most constructs was the same (5'-GGC GGA TCC AGC ACG CCC ATG CCC CCG- 3'), the COOH-terminal primer used to construct the dynamin-PRD GST-fusion proteins (see Figs. 2A and 3A) was as follows: full-length PRD, 5'-C GGG AAT TCC GTC GAA GGG GGG CCT GGG-3'; 751-848 (Fig. 2A, construct 4), 5'- C GGG AAT TCC ACC CGA TCG GCT GGG GAC C -3'; 751-832 (Figs. 2A and 3A, construct 5), 5'-C GGG AAT TCC CAC CTG AGG GGG AGG GCC-3'; 751-815 (Fig. 2A, construct 6), 5'-C GGG AAT TCC CAC GGG GGG CGC CCC CCC-3'; 751-798 (Fig. 2A, construct 7), 5'-C GGG AAT TCC AGG GCC CCG CGA CCC GGG-3'; 751-783 (Fig. 2A, construct 8), 5'-C GGG AAT TCC GCG CTG CGG CGT GGG GC-3'; 751-838 (Fig. 3A, construct 2), 5'-GC GCC GGG AAT TCC GCG GTT GGG GCG CGA GGG CAC C-3'; 751-838mut (Fig. 3A, construct 3), 5'-GC GCC GGG AAT TCC GCG GTT GGC GTC CGA GGG CAC C-3'; 751-827 (Fig. 3A, construct 6), 5'-CGG GAA TTC CGC CGA AAG GGT CAG GGG-3'. For construct 4 of Fig. 3A, which starts at the PSRPNR sequence, the following primers were used: NH2-terminal primer, 5'-GGC GGA TCC CAG GTG CCC TCG CGC CCC-3'; COOH-terminal primer, 5'-C GGG AAT TCC GTC GAA GGG GGG CCT GGG-3'.


Fig. 2. Affinity chromatography of total rat brain homogenate on dynamin I deletion constructs. A, schematic drawing of the human dynamin Iaa deletion constructs used for the affinity purification. PRD, proline-rich domain. B, protein staining (Coomassie) of the SDS-PAGE gels of total brain homogenate (TH) and of the material eluted from the affinity columns. Lane numbers correspond to the number of the constructs shown in A. Heavy bands represent fusion proteins used as the immunoabsorbents. An arrow points to the amphiphysin bands visible in lanes 3 and 4. C, Western blotting with anti-amphiphysin antibodies of a gel identical to the one shown in B. The two immunoreactive bands (lanes 3 and 4) correspond to the two bands indicated by an arrow in B.
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Fig. 3. Identification of the sequence PSRPNR as the amphiphysin binding site in dynamin I. A, schematic drawing of the human dynamin Iaa constructs (expressed as GST-fusion proteins) used for the experiments of panels B, C, and D. B, rat brain total homogenate (TH) and material affinity-purified on the GST dynamin constructs comprising the full-length proline-rich domain (PRD) and constructs 1-6 shown in panel A were probed by Western blotting with antibodies directed against amphiphysin, phospholipase C-gamma I, and Grb2. C, amphiphysin overlays of SDS-PAGE gels containing the GST-dynamin constructs 1-3 and 5 shown in panel A. Both in the affinity chromatography and in the overlay experiments, only the constructs including the PSRPNR sequence bind amphiphysin.
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Protein Interaction Studies

Affinity Chromatography of Brain Extracts

Soluble brain extracts were prepared as described (19). Recombinant proteins to be used as adsorbents were immobilized on glutathione-Sepharose 4B beads (Pharmacia Biotech Inc.) or on nickel-nitrilotriacetic acid-agarose (Qiagen). 50 µl of saturated beads were incubated with 1 ml of soluble protein extract (10 mg/ml total protein) for 4 h at 4 °C. Beads were washed extensively with incubation buffer and eluted with 10 mM glutathione in 50 mM Tris-HCl, pH 8.0 (GST proteins), or 250 mM imidazole (His-dynamin). Proteins were then separated on SDS-PAGE gels and reacted by Western blotting with various antibodies using the 4-chloro-1-naphthol reaction method.

Overlay Assays

GST-dynamin fusion proteins were separated on an SDS-PAGE gel, transferred to nitrocellulose, and incubated with His-amphiphysin (20 µg/ml) in 10 mM HEPES, 150 mM NaCl, 1 mg/ml bovine serum albumin, and 1% Triton X-100 for 2 h at room temperature. The nitrocellulose was then processed for bound amphiphysin immunoreactivity as described for Western blotting.

Combinatorial Library

An oriented peptide library (MAXXXXPXXPXXXAKKK, where X indicates all amino acids except Cys and Trp) synthesized as described (28) was used to investigate the specificity of the amphiphysin SH3 domain. GST-amphiphysin SH3 domain fusion protein, preadsorbed on glutathione-Sepharose beads, was incubated with the peptide library (1 mg) in phosphate-buffered saline (150 mM NaCl, 3 mM KCl, 10 mM Na2HP04, 2 mM KH2P04, pH 7.2), 1 mg/ml bovine serum albumin, and 0.01% Nonidet P-40 for 1 h at 4 °C. The beads were washed three times with phosphate-buffered saline. The peptides retained were eluted with 30% acetic acid, lyophilized, resuspended in distilled water, and sequenced as a mixture. To determine the relative selectivity of amino acids at each degenerate (X) position, the sequences of bound peptides were compared with those of control experiments using GST alone (28). Details concerning combinatorial library construction and screening can be found in Ref. 29.

Binding Assay

The GST-SH3 domain of amphiphysin was purified to homogeneity by affinity chromatography on glutathione-Sepharose followed by anion exchange chromatography on a Protein-Pack Q 8HR column (Waters) using a 0-350 mM NaCl gradient in 25 mM HEPES buffer, pH 7.0, on a fast protein liquid chromatography system (Pharmacia). 90 µg of purified protein was labeled with 125I using IODO-BEADS (Pierce) according to the manufacturer's instructions. The specific radioactivity of the labeled protein was determined as 1.772 × 106 cpm/µg, which corresponds to 7.974 × 107 cpm/nmol. A GST-fusion protein comprising the full-length proline-rich domain (PRD) of dynamin was coupled to Ultralink Biosupport medium (Pierce) at a concentration of 0.3 mg/ml. The radiolabeled GST-SH3 domain of amphiphysin was incubated with 5 µl of PRD beads in 100 µl of 20 mM HEPES, pH 7.2, 150 mM NaCl, 5 mg/ml bovine serum albumin, and 0.1% Tween 20 for 1 h at room temperature. Beads were collected by centrifugation in the Eppendorf microcentrifuge and washed twice with ice-cold reaction buffer. The radioactivity of the supernatant of the centrifugation (free) and beads (bound) was determined using a gamma -radiation counter. Specific binding was determined as the difference in binding in the absence and presence of a 100-molar excess of unlabeled amphiphysin SH3 domain. The affinity constant was calculated from the slope of the Scatchard plot of the binding data.

Miscellaneous Procedures

Preparation of total tissue homogenates and SDS-PAGE were performed according to Laemmli (30). Protein was measured using the BCA and Coomassie Plus protein assay kit (Pierce).


RESULTS

To determine whether the binding of amphiphysin to dynamin I is critically dependent on the classical SH3 domain binding interface, we generated a mutant amphiphysin SH3 domain harboring point mutations at amino acid positions 684 (glycine to arginine) and 687 (proline to leucine). Each of these two mutations were found to abolish the binding properties of the SH3 domains of the Grb2Sem5Drk protein (31). GST-fusion proteins comprising either the wild type or the mutant amphiphysin SH3 domains were incubated with brain extracts, and the proteins that selectively bound to these constructs were analyzed by SDS-PAGE and protein staining. As shown in Fig. 1, both dynamin I and synaptojanin were specifically affinity-purified by the wild type but not by the mutant SH3 domain.


Fig. 1. Affinity purification of total rat brain homogenate on GST-fusion proteins containing the wild type or mutant (Gly684 right-arrow Arg and Pro687 right-arrow Leu) SH3 domain of amphiphysin. Material specifically retained by the beads was separated by SDS-PAGE and stained by Coomassie Blue. The wild type (wt) but not the mutant (mut) SH3 domain binds dynamin I and synaptojanin. The identity of the two proteins was confirmed by Western blotting (not shown).
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To identify the binding site for amphiphysin SH3 domain in dynamin I, a variety of dynamin I deletion constructs were generated (Fig. 2, A and B), and their ability to affinity-purify amphiphysin from brain extract was assessed by Western blotting of the affinity-purified material (Fig. 2C). As shown in Fig. 2, B and C (lane 3), a GST-fusion protein containing the entire proline-rich domain of dynamin I binds amphiphysin, while GST alone or a truncated form of dynamin I missing the entire proline-rich region does not (Fig. 2, B and C, lanes 1 and 2), confirming that the interaction is mediated as expected by the proline-rich region of dynamin I. Progressive deletion of the COOH-terminal portion of the proline-rich region of dynamin I defined the segment comprising amino acids 832 and 848 as essential for amphiphysin binding (Fig. 2, compare lanes 4 and 5). Since amphiphysin is the major band selectively enriched in the material, which is affinity-purified by constructs 3 and 4 (lanes 3 and 4 of Fig. 2B, arrows), these data also confirm that amphiphysin is indeed one of the major dynamin-binding proteins in rat brain.

Further deletions within the proline-rich domain of dynamin I allowed definition of the sequence PSRPNR as the amino acid stretch required for the interaction (Fig. 3A, constructs 2 and 5; Fig. 3B, lanes 2 and 5). This result was corroborated by the demonstration that mutation of the arginine and proline within this segment into an aspartate and alanine, respectively (construct 3 of Fig. 3A), also abolished amphiphysin binding (Fig. 3B, lane 3).

A common characteristic of SH3 domain binding sites is the presence of the core motif PXXP, which is often followed by an arginine at position +5 (the first P of the core PXXP being position 0) in class II binding ligands for SH3 domains (32-35). Disruption of amphiphysin binding by the mutation of constructs 3 and 5 is therefore compatible with a binding of amphiphysin either to the sequence PSRPNR or to the overlapping sequence PQVPSR. However, amphiphysin was still able to bind (albeit less efficiently) to construct 4, which includes only the PSRPNR motif (Fig. 3A, construct 4; Fig. 3B, lane 4), confirming the importance of this amino acid sequence. The less effective binding of the amphiphysin to this construct is not surprising since there is evidence that SH3 domain binding can be modulated by amino acid sequences flanking the main SH3 domain binding site (34).

In contrast to these findings, all Grb2 binding activity was lost in constructs 4, 5, and 6, indicating that the PQVPSR motif is the major Grb2 binding site (Fig. 3B). Mutation or deletion of the PSRPNR sequence did not decrease binding of the SH3-containing proteins PLC-gamma 1 (36) (Fig. 3B) and cortactin (37) (not shown), which therefore must bind to sequences NH2-terminal to this site. The crucial role of the PSRPNR region for the amphiphysin-dynamin I interaction was confirmed by overlaying blots of dynamin I constructs (Fig. 3A) with recombinant polyhistidine-tagged amphiphysin (Fig. 3C). This experiment rules out the possibility that the interactions revealed by affinity chromatography of brain extracts (Fig. 2, B and C, and Fig. 3B) may be indirect.

We next used a combinatorial peptide library approach to identify the preferred binding motif of the SH3 domain of amphiphysin and its relation to the binding region identified in dynamin I. A combinatorial peptide library was incubated with the GST-amphiphysin SH3 domain, and the consensus amino acid sequence that binds the SH3 domain was established by sequencing of the bound peptides (Fig. 4). The consensus obtained was PXRPXR(H)R(H) in good agreement with the results of our binding studies.


Fig. 4. Peptide specificity (PXRPXR(H)R(H)) of the amphiphysin SH3 domain. The first fixed proline (underlined) in the library (MAXXXXXPXXPXXXAKKK) is considered as the 0 position. Panels A-F show selectivity values of amino acids in positions -1, +1, +2, +4, +5, and +6, respectively. Values that are higher than 1.5 indicate significant selection. No significant preference of amino acids was detected at other degenerate positions (not shown).
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To determine the affinity of the interaction between the proline-rich domain of dynamin I and the SH3 domain of amphiphysin we tested a 125I-labeled GST-SH3 domain of amphiphysin for its binding to immobilized GST-fusion protein comprising the proline-rich domain of dynamin I (construct 3 of Fig. 2A). The results are shown in Fig. 5. They are consistent with a single-site binding model and demonstrate a high affinity interaction with a dissociation constant of 190 ± 10 nM. Since the putative preferred binding sites for amphiphysin and Grb2 are partially overlapping in the proline-rich domain of dynamin, we tested whether the SH3 domain of amphiphysin and Grb2 can compete for binding to dynamin I. As shown in Fig. 6, Grb2 prevented the binding of amphiphysin, confirming the close localization of the binding sites for these two proteins in the dynamin I molecule.


Fig. 5. High affinity interaction of the amphiphysin SH3 domain with the proline-rich domain of dynamin I. A, binding of 125I-labeled GST-amphiphysin SH3 domain to a GST-fusion protein comprising the proline-rich domain of dynamin I (construct 3 of Fig. 2A) covalently coupled to the Ultralink beads. Data represent the average of two independent experiments with duplicated points. B, Scatchard plot of the binding data in panel A.
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Fig. 6. Grb2 competes with amphiphysin SH3 domain for binding to dynamin I. A GST-fusion protein comprising the proline-rich sequence of dynamin I (construct 3 of Fig. 2A) was incubated with 400 nM radiolabeled GST-amphiphysin SH3 domain in the absence (1) or presence (2) of 40 µM GST-Grb2-fusion protein.
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DISCUSSION

Dynamin I contains a proline-rich COOH-terminal region of about 100 amino acids that interacts in vitro and in vivo with a variety of SH3 domain-containing proteins, including PLC-gamma , the p85 subunit of phosphatidylinositol 3-kinase, Src, Grb2, and amphiphysin (15, 16, 19, 23, 37). The multiplicity of the binding partners for this domain revealed by in vitro and in vivo studies may be explained by either a promiscuous interaction of various SH3 domains with several proline-rich stretches or by the presence in the tail of dynamin I of an array of distinct sites, each of which specifically binds certain SH3 domains. We report here that amphiphysin, a binding partner for dynamin I in nerve endings (19), binds with remarkable specificity and high affinity to a unique site in its long proline-rich domain.

The sequence PSRPNR at the amino acid position from 833 to 838 was found to be critically required for amphiphysin binding. Strikingly, this sequence matches the consensus binding motif of the amphiphysin-SH3 domain very well as revealed by a combinatorial peptide library: PXRPXR(H)R(H). The only difference between the dynamin I binding site and this consensus is the presence of an alanine rather than an arginine at position +6 (see Fig. 3A). However, the position +6 is clearly not essential for dynamin I binding as shown by the similar binding of constructs 1 and 2 of Fig. 3A. This is a novel SH3 binding consensus that would predict a class II type orientation of the peptide, given the presence of an arginine at the COOH-terminal side (position +5) of the core (32-35). We note that an amino acid sequence that fits the consensus PXRPXR is present in synaptojanin, the other major amphiphysin binding protein in brain (23).

The PSRPNR sequence is placed at the COOH-terminal end of a longer proline-rich stretch in dynamin I (Fig. 3A) that contains the sequence PQVPSR. This sequence was recently reported to fit the consensus for binding to the NH2-terminal SH3 domain of Grb2 and to the SH3 domain of Src (35). While the core of these other consensus motifs is the PQVP sequence, our results suggest that the core of the amphiphysin binding site is located three amino acids closer to the COOH terminus and corresponds to the PSRP sequence. We do, however, provide support to the hypothesis that the PQVP motif represents the core of the major Grb2 binding site, which is consistent with the mapping of the Grb2 binding region in dynamin I reported by other groups (15, 18). Accordingly, the ability of Grb2 to compete with amphiphysin for binding to dynamin I supports a close localization of the binding sites for these two proteins.

The sequence PSRPNR is clearly not required for the binding of dynamin I to the SH3 domain of PLC-gamma , in agreement with previous results indicating that PLC-gamma and the p85 subunit of phosphatidylinositol 3-kinase bind to an amino acid sequence upstream of this region (amino acids 778-795) (15, 36). On the other hand, a dynamin sequence including the PSRPNR site was proposed to be a PLC-gamma binding site based on combinatorial peptide library data (35). These results stress the importance of complementing information on SH3 domain consensus sequences obtained using small peptides with studies on longer protein stretches. These findings provide support to the hypothesis that the long proline-rich COOH-terminal region of dynamin I represents a template for the recruitment of different SH3-containing proteins via highly specific interactions mediated by distinct binding sites. The proline-rich domain of dynamin I undergoes phosphorylation/dephosphorylation (38). It is possible that the phosphorylation of specific amino acid positions may serve to selectively regulate the interactions of dynamin I with one or a subset of SH3 domains.

Many of the SH3 domain-containing proteins that interact with dynamin I can also interact via distinct domains with proteins that are concentrated at plasmalemmal clathrin-coated pits. For example, PLC-gamma , the p85 subunit of phosphatidylinositol 3-kinase and Grb2, also contains SH2 domains that bind to tyrosine-phosphorylated growth factor receptors (39). Amphiphysin interacts via a region distinct from its SH3 domain with the clathrin adaptor AP2 (19, 24). The presence of binding sites for these various proteins in the tail of dynamin I may serve to guarantee an efficient recruitment of dynamin I at clathrin-coated buds (19) where dynamin I plays a key role in the fission reaction (13). Conversely, due to its property of interacting with multiple SH3 domains dynamin I itself may serve to further recruit other SH3 domain-containing proteins in a positive feedback loop. In agreement with these considerations, we have recently shown that disruptions of the SH3-mediated interactions of dynamin in living nerve terminal preparations potently inhibits the fission reaction of clathrin-coated vesicles (40). Furthermore, a previous study has shown that an SH3 domain binding motif in dynamin I is required for its targeting to clathrin-coated pits in COS7 cells (41). This motif was mapped to a site upstream to the sequence PSRPNR described here, which could be deleted without abolishing dynamin I targeting in these cells (41). However, the SH3 domain of amphiphysin may play a dominant role in the recruitment of dynamin I at clathrin-coated pits involved in synaptic vesicle reformation, while other SH3 domain-containing proteins may play a dominant role at other types of clathrin-coated pits.

The data here open the possibility of generating mutant dynamin I molecules that are selectively impaired in their property of binding the SH3 domain of amphiphysin and therefore studying the precise role of this interaction in nerve terminal function.


FOOTNOTES

*   This work was supported by grants from the Donaghue Foundation, the Human Frontier Science Programme, and the National Institutes of Health (CA46128) (to P. D. C.) and by postdoctoral fellowships from the Deutscher Akademischer Austauschdienst (to D. G.), the Muscular Dystrophy Association (to V. I. S.), and the United States Army Medical Research and Development Command (to C. D.).
§   Present address: Institut fuer Anatomie und Spezielle Embryologie, Universitaet Fribourg, CH-1700 Fribourg, Switzerland.
par    Present address: Dept. of Immunology, Weizmann Inst. of Science, Rehovot, Israel.
Dagger Dagger    To whom correspondence should be addressed: Dept. of Cell Biology and Howard Hughes Medical Inst., Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Ave., New Haven, CT 06510. Tel.: 203-737-4461; Fax: 203-737-1762; E-mail: pietro_decamilli{at}quickmail.yale.edu.
1   The abbreviations used are: PLC, phospholipase C; GST, glutathione S-transferase; PAGE, polyacrylamide gel electrophoresis; PRD, proline-rich domain.

ACKNOWLEDGEMENTS

We thank Dr. S. Schmid for the gift of dynamin clones and Drs. T. Parson and S. G. Rhee for the gift of antibodies.


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