(Received for publication, November 25, 1996)
From the Takai Biotimer Project, ERATO, Japan Science
and Technology Corporation, c/o JCR Pharmaceuticals Co., Ltd., 2-2-10 Murotani, Nishi-ku, Kobe 651-22, Japan, the § Department of
Molecular Biology and Biochemistry, Osaka University Medical School,
Suita 565, Japan, and the
Department of Virology II, National
Institute of Health, Tokyo 162, Japan
The Rab small G protein family, consisting of nearly 30 members, is implicated in intracellular vesicle trafficking. They cycle between the GDP-bound inactive and GTP-bound active forms, and the former is converted to the latter by the action of a GDP/GTP exchange protein (GEP). No GEP specific for each Rab family member or Rab subfamily has been isolated. Here we purified a GEP from rat brain with lipid-modified Rab3A as a substrate. The purified protein was specifically active on Rab3A, Rab3C, and Rab3D of the Rab3 subfamily. Of these subfamily members, Rab3A and Rab3C are implicated in Ca2+-dependent exocytosis, particularly in neurotransmitter release. This GEP (Rab3 GEP) was active on the lipid-modified form, but not on the lipid-unmodified form. Rab3 GEP showed a minimum molecular mass of about 200 kDa on SDS-polyacrylamide gel electrophoresis. We cloned its cDNA from a rat brain cDNA library and determined its primary structure. The isolated cDNA encoded a protein with a Mr of 177,982 and 1,602 amino acids, which showed no homology to any known protein. The recombinant protein exhibited GEP activity toward Rab3A, Rab3C, and Rab3D. Northern blot and Western blot analyses indicated that Rab3 GEP was expressed in all the rat tissues examined with the highest expression in brain.
The Rab small G protein family consists of nearly 30 members and implicated in intracellular vesicle trafficking, such as exocytosis, endocytosis, and transcytosis (for reviews, see Refs. 1-6). All the Rab family members have unique C-terminal structures, which undergo posttranslational modifications with geranylgeranyl moieties in most cases. The Rab family members cycle between the GDP-bound inactive and GTP-bound active forms and between the cytosol and membrane fractions. These two types of cycling are essential for their action in vesicle trafficking. The conversion from the GDP-bound form to the GTP-bound form is regulated by two types of regulatory proteins; one is Rab GEP,1 which stimulates this conversion, and the other is Rab GDI, which inhibits this conversion. The conversion of the GTP-bound form to the GDP-bound form is regulated by Rab GAP. Rab GDI has been isolated and well characterized (6). Rab GDI interacts specifically with the GDP-bound form of all the Rab family members thus far examined and keeps them both in the GDP-bound form and in the cytosol or releases them from the membranes. In contrast to Rab GDI, little is known about Rab GEP and Rab GAP. As for Rab GEP, a yeast GEP, named DSS4, and its mammalian counterpart, named MSS4, have thus far been reported, but MSS4 is not specific for a Rab family member or Rab subfamily (7-10). No GEP specific for each Rab family member or Rab subfamily has been isolated.
The Rab3 subfamily consists of four members, Rab3A, Rab3B, Rab3C, and Rab3D (6). Of these members, Rab3A and Rab3C are implicated in Ca2+-dependent exocytosis, particularly in neurotransmitter release. A GEP active on Rab3A has been partially purified from rat brain, but neither its primary structure nor its precise property has been studied (10-12). Therefore, we have attempted here to isolate a GEP specific for Rab3A or the Rab3 subfamily.
Lipid-modified Rab3A, Rab3B, Rab3C, Rab3D, Rab2, Rab5A, Rab10, and Rab11 were purified from the membrane fraction of Sf9 cells expressing each cDNA (13, 14). Lipid-unmodified Rab3A was purified from Rab3A-overexpressing Escherichia coli as a fusion protein with N-terminal glutathione S-transferase, of which the glutathione S-transferase carrier was cleaved off from Rab3A by digestion with thrombin (15). MSS4 was purified from MSS4-overexpressing E. coli as described (10). Rab GDI was purified from bovine brain cytosol (16).
Assay for Rab3 GEP ActivityThe Rab3 GEP activity was
assayed by measuring the dissociation of [3H]GDP from
lipid-modified Rab3A as follows: Rab3A (3 pmol) was incubated for 20 min at 30 °C with 3 µM [3H]GDP in a
reaction mixture (5 µl) containing 50 mM Tris/Cl at pH
8.0, 5 mM MgCl2, 10 mM EDTA, 0.5 mM DTT, and 0.12% CHAPS. The reaction was stopped by
adding 2 µl of 100 mM MgCl2 and 5 µl of a
solution containing 50 mM Tris/Cl at pH 8.0, 5 mM MgCl2, 0.5 mM EDTA, and 1 mM DTT. The sample to be assayed was incubated for 10 min
at 30 °C with [3H]GDP bound to Rab3A in a reaction
mixture (50 µl) containing 50 mM Tris/Cl at pH 8.0, 12 mM MgCl2, 2 mM EDTA, 0.2 mg/ml
bovine serum albumin, 12 µM GTPS, and 0.06% CHAPS.
The mixture was applied to a nitrocellulose filter, and the
radioactivity retained on the filter was determined by counting. The
Rab3 GEP activity to stimulate the binding of
[35S]GTP
S to lipid-modified Rab3A was assayed as
described above, except that [3H]GDP and GTP
S were
replaced with GDP and [35S]GTP
S, respectively.
All the purification procedures
were performed at 0-4 °C. The synaptic soluble fraction was
prepared from 80 rat brains (17). A half of the fraction (500 ml, 455 mg of protein) was adjusted to 0.2 M NaCl and applied to a
Q-Sepharose FF column (2.6 × 10 cm) equilibrated with Buffer A
(20 mM Tris/Cl at pH 7.5 and 1 mM DTT)
containing 0.2 M NaCl. Elution was performed with 350 ml of
Buffer A containing 0.5 M NaCl. Fractions of 10 ml each were collected. The Rab3 GEP activity appeared in Fractions 5-19. These fractions (150 ml, 159 mg of protein) were collected, and NaCl
was added to give a final concentration of 2 M. The sample was applied to a phenyl-Sepharose column (2.6 × 10 cm)
equilibrated with Buffer A containing 2 M NaCl. Elution was
performed with a 360-ml linear gradient of NaCl (2-0 M) in
Buffer A, followed by 180 ml of Buffer A. Fractions of 6 ml each were
collected. The Rab3 GEP activity appeared in Fractions 52-63. These
fractions (72 ml, 8.6 mg of protein) were collected and applied to a
hydroxyapatite column (1.0 × 30 cm) equilibrated with Buffer B
(20 mM potassium phosphate at pH 7.8, 1 mM DTT,
0.6% CHAPS, and 10% glycerol). Elution was performed with a 75-ml
linear gradient of potassium phosphate (20-100 mM) in
Buffer B and a subsequent 75-ml linear gradient (100-300
mM) in Buffer B, followed by a 50-ml linear gradient
(300-500 mM) in Buffer B. Fractions of 2.5 ml each were collected. The Rab3 GEP activity appeared in Fractions 46-54. These
fractions (22.5 ml, 2.2 mg of protein) were collected, diluted with an
equal volume of Buffer C (20 mM bis-Tris/Cl at pH 5.5, 0.5 mM EDTA, 1 mM DTT, 0.6% CHAPS, and 10%
glycerol), and applied to a Mono Q HR 10/10 column equilibrated with
Buffer C. Elution was performed with a 60-ml linear gradient of NaCl
(0.2-0.5 M) in Buffer C. Fractions of 1 ml each were
collected. The Rab3 GEP activity appeared in Fractions 24-33. These
fractions (10 ml, 0.44 mg of protein) were collected, concentrated to
about 2 ml, and applied to a Superdex 200 column (1.6 × 60 cm)
equilibrated with Buffer D (20 mM Tris/Cl at pH 7.5, 0.5 mM EDTA, 1 mM DTT, 0.6% CHAPS, 0.45% sodium
cholate, 10% glycerol, and 0.15 M NaCl). Elution was
performed with the same buffer. Fractions of 2 ml each were collected.
The Rab3 GEP activity appeared in Fractions 26-30 (see Fig.
1A). These active fractions (10 ml, 45 µg of protein) were
collected. The other half of the synaptic soluble fraction was also
subjected to the successive column chromatographies in the same manner
as described above. The active fractions of the two Superdex 200 column
chromatographies were combined and applied to a high performance liquid
chromatography hydroxyapatite column (Koken Co. Ltd., Tokyo, 0.78 × 10 cm) equilibrated with Buffer B. Elution was performed with a
12.5-ml linear gradient of potassium phosphate (20-100 mM)
in Buffer B, followed by a 50-ml linear gradient of potassium phosphate
(100-500 mM) in Buffer B. Fractions of 1 ml each were
collected. The Rab3 GEP activity appeared in two peaks in Fractions
29-33 and 34-38 (see Fig. 1B). The first (5 ml, 15.5 µg
of protein) and second (5 ml, 7.5 µg of protein) peaks were
separately collected as Rab3 GEPI and GEPII, respectively, and stored
at 80 °C.
Peptide Mapping of Rab3 GEP and Molecular Cloning of the Rab3 GEP cDNA
Purified Rab3 GEPII (20 µg of protein) and GEPI (10 µg of protein) were separately subjected to SDS-PAGE (6.5%
polyacrylamide gel). Each protein band corresponding to a protein with
a molecular mass of about 200 kDa was cut out from the gel, digested
completely with a lysyl endopeptidase, and subjected to C18 reverse
phase high pressure liquid column chromatography (18). The amino acid sequences of the peptides were determined with a peptide sequencer. To
determine the N-terminal amino acid sequence of Rab3 GEPII, purified
GEPII (4 µg of protein) was subjected to SDS-PAGE and transferred to
a polyvinylidene difluoride membrane. The protein band was cut from the
membrane and directly subjected to the peptide sequencer. A rat brain
cDNA library in ZAPII (Stratagene) was screened using the
oligonucleotide probes designed from the partial amino acid sequences
(19). DNA sequencing was performed by the dideoxy nucleotide
termination method using an ABI373 DNA sequencer.
The cDNA of Rab3 GEP was cloned into the pCMV vector and the construct was transfected to COS7 cells with the DEAE-dextran method (20). The COS7 cells were homogenized with a buffer containing 20 mM Tris/Cl at pH 7.5, 1 mM DTT, 0.6% CHAPS, and centrifuged at 100,000 × g for 1 h. The supernatant (2 ml, 4.2 mg of protein) was subjected to Mono Q PC 1.6/5 column chromatography. Each fraction was assayed for the Rab3 GEP activity. The active fractions were collected and used as recombinant Rab3 GEP.
Rab3 GEP was purified from the synaptic soluble fraction of rat brain with lipid-modified (geranylgeranylated and methylated) Rab3A as a substrate by column chromatographies, including Q-Sepharose, phenyl-Sepharose, hydroxyapatite, Mono Q, and Superdex 200 column chromatographies. On these column chromatographies, Rab3 GEP appeared in a single peak. The GEP activity well coincided with one protein with a molecular mass of about 200 kDa on the last column chromatography (Fig. 1A). When this sample was further subjected to re-hydroxyapatite column chromatography, Rab3 GEP appeared in two peaks (Rab3 GEPI and GEPII), but its activity of both peaks well coincided with proteins with a molecular mass of about 200 kDa (Fig. 1B).
Rab3 GEPII was inactive on other Rab subfamily members, including Rab2,
Rab5A, Rab10, and Rab11 (Fig. 2A). Rab3 GEPII
was active on Rab3A and Rab3C, and partially active on Rab3D, but was
nearly inactive on Rab3B (Fig. 2B). Rab3 GEPII was active on
lipid-modified Rab3A, but not on the lipid-unmodified form (Fig.
3A). These properties of Rab3 GEPII were
different from those of MSS4 which was equally active on lipid-modified
and -unmodified Rab3A and active on many other Rab family members,
including Rab3A, Rab3C, Rab3D, Rab10, and Rab11 (Figs. 2 and
3A). Rab3 GEPII as well as MSS4 was inactive on Rab3A
complexed with Rab GDI (Fig. 3B). The properties of Rab3
GEPI, including the requirement for lipid modifications of Rab3A, the
substrate specificity, and the sensitivity to Rab GDI, were similar to
those of Rab3 GEPII described above (data not shown).
Both the Rab3 GEPI and GEPII proteins with molecular masses of about
200 kDa were accumulated from 800 rat brains by the same series of
column chromatographies as described above, and their peptide maps were
determined. The peptide maps of these proteins were apparently
identical (data not shown). Therefore, the amino acid sequences of the
nine peptides of Rab3 GEPII were determined. The N-terminal amino acid
sequence of Rab3 GEPII was further determined. On the basis of these
amino acid sequences, we cloned a cDNA from a rat brain cDNA
library and determined its nucleotide sequence (accession number
U72995[GenBank]). The deduced amino acid sequence included all the amino acid
sequences of the peptides (Fig. 4). The initial
methionine residue appears to be cleaved off after the translation. The
encoded protein consisted of 1,602 amino acids and showed a calculated
Mr of 177,982. Computer homology search revealed
homology to proteins encoded by Caenorhabditis elegans
cDNA yk26 g7.5 (accession number U49945[GenBank]) and by human DENN
(accession number U44953[GenBank]), of which functions are unknown. The deduced
amino acid sequence of yk26 g7.5 protein showed 35% identity over the
entire sequence to that of Rab3 GEP. The deduced amino acid sequence of
human DENN protein is almost identical over the entire sequence to that
of Rab3 GEP, while human DENN protein lacks about 300 C-terminal amino
acids. The exact relationship between Rab3 GEPI and GEPII is not known,
but it is most likely that Rab3 GEPI is a splicing isoform of Rab3 GEPII, since several splicing isoforms were isolated.
The recombinant protein was prepared from the Rab3 GEP cDNA-transfected COS7 cells. The properties of the recombinant Rab3 GEP, including the requirement for lipid modifications of Rab3A, the substrate specificity, and the sensitivity to Rab GDI, were similar to those of the native Rab3 GEPII described above (data not shown). Northern blot and Western blot analyses indicated that Rab3 GEP was expressed in all the rat tissues examined, including heart, brain, spleen, lung, liver, skeletal muscle, kidney, and testis, with the highest expression in brain (data not shown).
We have isolated here for the first time a GEP specific for one Rab subfamily, the Rab3 subfamily, determined its primary structure, and characterized it. Our Rab3 GEP is the most active on Rab3A and Rab3C and partially on Rab3D, but inactive on Rab3B. This substrate specificity is apparently consistent with the similar properties of Rab3A and Rab3C concerning their tissue and subcellular distributions and functions (6, 21). Rab3A and Rab3C are present in cells with a regulated secretion pathway and abundant in brain where both are highly concentrated on synaptic vesicles. Both Rab3A and Rab3C have been implicated in Ca2+-dependent exocytosis, particularly in neurotransmitter release. The tissue distributions of Rab3B and Rab3D are different from those of Rab3A and Rab3C, and their functions remain to be clarified. Because Rab3 GEP is inactive on Rab3B, Rab3B may have its own specific GEP which may be different from Rab3 GEP. GEPs for other small G proteins, including the Ras and Rho family members, have been isolated and characterized (for a review, see Ref. 22). GEPs for the Ras and Rho family members share the common catalytic domains specific for each family. Our Rab3 GEP does not have any homologous region to these GEPs. Our present result that Rab3 GEP is specific for the Rab3 subfamily members suggests that each Rab family member or Rab subfamily has their own specific GEP. It is important to isolate GEPs specific for each Rab family member or Rab subfamily and to know whether they have a common catalytic domain or their own specific catalytic domain.
Rab GDI functions as a regulatory protein for the two types of cycling of the Rab family members between the GDP-bound and GTP-bound forms and between the cytosol and membrane fractions (6). We have shown here that Rab3 GEP is inactive on Rab3A complexed with Rab GDI, suggesting that another factor is further necessary for the conversion. A factor, named GDF, has been shown to be necessary for the dissociation of GDP-Rab5 and GDP-Rab9 from Rab GDI (23, 24). Although Rab5 or Rab9 GDF has not been identified, it has been suggested to be located on the membrane fraction (23, 24). We have shown previously that GDP-Rab3A complexed with Rab GDI stays in the cytosol of nerve terminals, and that rabphilin3 is associated with synaptic vesicles (6). We have recently detected a GDF activity to Rab3A in isolated synaptic vesicles.2 Taken together, the conversion from the GDP-bound form to the GTP-bound form occurs in the proximity of synaptic vesicles where once the GTP-bound form is produced, it is immediately transferred to rabphilin3. It may be noted that Rab3 GEP as well as Rab GDI requires the posttranslational lipid-modifications of Rab3A, whereas rabphilin3 does not. The lipid moieties of Rab3A may be masked by both Rab GDI and Rab3 GEP until it is converted to the GTP-bound form. Once the GTP-bound form is produced, Rab3A interacts with synaptic vesicles through both protein-protein (Rab3A-rabphilin3) and lipid-lipid (geranylgeranyl-vesicle phospholipid) interactions. Thus, the lipid modifications of Rab3A are important to determine the intracellular compartment of Rab3A, its regulators, and target.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) U72995[GenBank].
We thank Dr. M. Kasuga (Kobe University School of Medicine, Kobe, Japan) for providing us the baculovirus carrying the Rab3D cDNA, Dr. D. W. Russell (University of Texas Southwestern Medical Center at Dallas, Dallas, TX) for the pCMV vector, and Dr. M. Zerial (EMBL, Heidelberg, Germany) for the cDNAs of Rab2, Rab5A, and Rab10.