Mechanistic Studies on the Inactivation of the Proteasome by Lactacystin in Cultured Cells*

(Received for publication, July 1, 1996, and in revised form, September 15, 1996)

Lawrence R. Dick Dagger , Amy A. Cruikshank , Antonia T. Destree , Louis Grenier , Teresa A. McCormack , Francesco D. Melandri , Sandra L. Nunes , Vito J. Palombella , Lana A. Parent , Louis Plamondon and Ross L. Stein

From ProScript, Inc., Cambridge, Massachusetts 02139

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS AND DISCUSSION
FOOTNOTES
REFERENCES


ABSTRACT

The natural product lactacystin exerts its cellular antiproliferative effects through a mechanism involving acylation and inhibition of the proteasome, a cytosolic proteinase complex that is an essential component of the ubiquitin-proteasome pathway for intracellular protein degradation. In vitro, lactacystin does not react with the proteasome; rather, it undergoes a spontaneous conversion (lactonization) to the active proteasome inhibitor, clasto-lactacystin beta -lactone. We show here that when the beta -lactone is added to mammalian cells in culture, it rapidly enters the cells, where it can react with the sulfhydryl of glutathione to form a thioester adduct that is both structurally and functionally analogous to lactacystin. We call this adduct lactathione, and like lactacystin, it does not react with the proteasome, but can undergo lactonization to yield back the active beta -lactone. We have studied the kinetics of this reaction under appropriate in vitro conditions as well as the kinetics of lactathione accumulation and proteasome inhibition in cells treated with lactacystin or beta -lactone. The results indicate that only the beta -lactone (not lactacystin) can enter cells and suggest that the formation of lactathione serves to concentrate the inhibitor inside cells, providing a reservoir for prolonged release of the active beta -lactone.


INTRODUCTION

Lactacystin is a natural product that was originally discovered for its ability to induce neurite outgrowth and differentiation in a mouse neuroblastoma cell line, Neuro 2A (1). Subsequent work (2, 3) demonstrated that the biological effects of lactacystin result from its ability to acylate and inhibit the proteasome, a ubiquitous intracellular protein-degrading machine (for reviews, see Refs. 4, 5, 6, 7, 8, 9, 10). Because the proteasome is involved both in the normal turnover of cellular proteins (11) and in the processing and degradation of regulatory proteins that control cell growth and metabolism (12), proteasome inhibitors can have profound biological consequences.

As part of a program to develop proteasome inhibitors into novel therapeutic agents, we have undertaken detailed studies of the mechanism of lactacystin. In a recent report (13), we demonstrated that lactacystin per se is not a proteasome inhibitor. Rather, lactacystin in aqueous solution can spontaneously undergo an intramolecular reaction (lactonization) to form the active proteasome inhibitory species, clasto-lactacystin beta -lactone. Herein we have extended these studies to examine the mechanism of proteasome inhibition by lactacystin in cultured cells. We show that cells are relatively impermeable to lactacystin, but highly permeable to beta -lactone. Thus, the efficacy of lactacystin as a proteasome inhibitor in cells will depend on the lactonization of lactacystin in the medium to generate beta -lactone. Once inside the cell, beta -lactone has multiple fates. It can hydrolyze to the inactive dihydroxy acid, it can react with the proteasome, or it can react with GSH to form a GSH conjugate with properties that are analogous to lactacystin. This GSH conjugate, which we call lactathione, is inactive as a proteasome inhibitor. Nevertheless, like lactacystin, it can spontaneously regenerate the active beta -lactone via intramolecular lactonization. These findings bring forth interesting questions pertaining to the relationship between the metabolism of beta -lactone and its efficacy as a proteasome inhibitor in vivo. In addition, the data point to several practical considerations for the ever increasing use of these compounds to study the biological function of proteasomes.


EXPERIMENTAL PROCEDURES

Synthesis

Lactacystin and clasto-lactacystin beta -lactone were prepared by the method of Corey et al. (14, 15). Lactathione was prepared by mixing beta -lactone (49 mg, 0.229 mmol) in 10 ml of acetonitrile with glutathione (360 mg, 1.17 mmol) and 1.35 ml of 1.00 N NaOH (1.35 mmol). The mixture was stirred for 4 h at room temperature, and 1 N HCl was added to obtain pH 4. The solution was concentrated on a rotovap and then lyophilized. The dry material was resuspended in a small volume of water and subjected to reverse-phase HPLC1 on a 19 × 300-mm Delta-Pak C18 column (Waters). Lactathione and the lactathione isomer (see Fig. 1) were collected in separate fractions eluting from the column. Lyophilization of the fractions afforded 48 mg of lactathione and 7.8 mg of the lactathione isomer. The structure of the synthetic materials was confirmed by proton magnetic resonance spectrometry and electrospray ionization mass spectrometry. beta -[3H]Lactone at a specific activity of 3.4 Ci/mmol was prepared from beta -lactone by the method described (3).


Fig. 1. Reaction of clasto-lactacystin beta -lactone with GSH. A, 10 µl of 20 mM beta -lactone in acetonitrile was mixed with 10 µl of 0.1 M GSH in water at pH 7.5. After 6 min at room temperature, the sample was diluted with 980 µl of 0.06% trifluoroacetic acid in water. A 100-µl aliquot of the sample was subjected to reverse-phase HPLC as described under "Experimental Procedures." B, shown is a chromatogram of GSH incubated in the absence of beta -lactone. C, shown is a chromatogram of beta -lactone incubated in the absence of GSH.
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HPLC Analyses of Lactathione and Lactacystin

The HPLC methodology has been described in detail (13). Briefly, the stationary phase was a C18 column (Vydac, 218TP54), and the mobile phase consisted of a gradient of methanol (0.05% trifluoroacetic acid) in water (0.06% trifluoroacetic acid).

Analysis of Lactathione Hydrolysis

The values for the rate constants given in Scheme 1 were estimated based on measurements of the GSH dependence of the steady-state rate constant for lactathione hydrolysis in phosphate-buffered saline at pH 7.4 and 37 °C and on separate measurements of beta -lactone hydrolysis under the same conditions. The spectrophotometric assays and data analysis were the same as those used in a previous work to study lactacystin hydrolysis (13).


Scheme 1. Mechanism of aqueous hydrolysis of lactathione.
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Lactathione Inactivation of Proteasome Activity

The 20 S proteasome and PA28 activator were purified from rabbit reticulocytes as described (13). Hydrolysis of the substrate succinyl-Leu-Leu-Val-Tyr 7-amino-4-methylcoumarin by the 20 S proteasome-PA28 complex was assayed by continuously monitoring the fluorescence of the liberated 7-amino-4-methylcoumarin (13). kinact values for lactathione were estimated by analysis of progress curves obtained upon addition of lactathione (13) at a final concentration of 2 µM.

General Cell Culture

Cell lines were obtained from American Type Culture Collection. Jurkat cells (human T cell leukemia, clone E6-1) were grown in RPMI 1640 medium (JRH Biosciences) supplemented with 10% heat-inactivated FBS (Sigma) and antibiotics (1000 units/ml penicillin and 100 µg/ml streptomycin) (Sigma). HeLa cells (human erythroid carcinoma) were grown in Dulbecco's modified Eagle's medium (DME/HIGH modified, JRH Biosciences) supplemented with 10% FBS and antibiotics as described above. C2C12 cells (mouse myoblast) were grown in Dulbecco's modified Eagle's medium (DME/HIGH modified) supplemented with 10% heat-inactivated FBS and antibiotics described as above. All cells were grown at 37 °C in an atmosphere of 5% CO2. Cells were passaged biweekly and used for experimental purposes in exponential growth phase.

GSH Depletion Studies

For GSH depletion, Jurkat cells were kept at low density (<= 5 × 106 cells/ml) for 24 h in the presence of 1 mM buthionine sulfoxime (16). Following this treatment, the cells were harvested, resuspended in fresh medium, and aliquoted into 24-well plates at a density of 5 × 107 cells/ml for measurement of lactathione accumulation or 7 × 106 cells/ml for measurement of IL-2 production. Aliquots of 2 × 106 cells were harvested and washed, and total intracellular glutathione was measured enzymatically using the GSH-GSSG recycling assay (17).

Extraction of Lactathione from Cells

Following exposure to beta -lactone or lactacystin at the concentrations and times indicated in the figure legends, cells were harvested and washed twice with cold PBS. To each cell pellet (5 × 107 cells) in a microcentrifuge tube was added 200 µl of cold methanol with 0.1% trifluoroacetic acid, and the pellet was homogenized with a motorized pestle. The tubes were centrifuged at 16,000 × g for 10 min at 4 °C. The supernatant was collected and dried in a vacuum centrifuge. The dried samples were resuspended in water with 0.06% trifluoroacetic acid and subjected to reverse-phase HPLC (see above).

Assay of 20 S Proteasome Activity in Crude Cell Extracts

Jurkat cells at 7 × 106 cells/ml in medium were treated for 30 min with different concentrations of beta -lactone. The cells were harvested and washed twice with cold PBS. The resulting pellets were ground with a motorized pestle in 50 µl of cold 5 mM HEPES, pH 7.5, with 1 mM dithiothreitol. The samples were centrifuged for 10 min at 16,000 × g in the cold, and the supernatants were collected. The total soluble protein was measured using the Coomassie Plus Protein Assay (Pierce). For measurement of 20 S proteasome peptidase activity, 10 µl of each sample (40-80 µg of total protein) was diluted into a cuvette containing 2 ml of 20 mM HEPES, 0.5 mM EDTA, pH 8, at 37 °C with 0.035% (w/v) SDS and 10 µM succinyl-Leu-Leu-Val-Tyr 7-amino-4-methylcoumarin. The substrate hydrolysis was measured by continuously monitoring the fluorescence of the liberated 7-amino-4-methylcoumarin. After 3-4 min, lactacystin was added to a final concentration of 10 µM, and the substrate hydrolysis was monitored for another 6-8 min. This lactacystin treatment is sufficient to inhibit the peptidase activity of purified 20 S proteasome by >= 99% under the same assay conditions (data not shown). Thus, the residual activity seen in crude extracts following lactacystin treatment presumably represents background from some other peptidase(s). This background was typically <= 5% of the total activity and was subtracted out.

Assay of Protein Turnover in C2C12 Cells

C2C12 cells were aliquoted into 12-well plates at 2 × 104 cells/well and grown overnight. The medium was exchanged with methionine-free Dulbecco's modified Eagle's medium (Life Technologies, Inc.) containing 10% dialyzed FBS and 1 µCi of [35S]methionine. The cells were grown for 48 h in the labeling medium and then washed three times with cold PBS containing 2 mM methionine. Fresh medium containing 10% FBS and 2 mM methionine was then added, and the cells were incubated for 2 h. The cells were washed once with PBS containing 2 mM methionine and cultured for an additional 4 h with medium containing 50% FBS, 2 mM methionine, and various concentrations of beta -lactone or lactacystin as indicated in Fig. 5. The media were harvested into microcentrifuge tubes, trichloroacetic acid was added to a final concentration of 10% (v/v), and the tubes were placed on ice for 15 min. The tubes were centrifuged at 16,000 × g for 5 min in the cold, and 100-µl aliquots of the resulting supernatants were subjected to liquid scintillation counting.


Fig. 5. Inhibition of the proteasome in cells by beta -lactone and lactacystin. A, Jurkat cells were treated for 30 min with various concentrations of beta -lactone. The cells were harvested, washed, and extracted to obtain soluble protein. 20 S proteasome peptidase activity in the extracts was determined as described under "Experimental Procedures." B, the proteins in C2C12 cells were metabolically labeled by culturing the cells for 2 days in the presence of [35S]methionine. Following a 2-h chase, fresh medium containing various concentrations of lactacystin or beta -lactone was put on the cells, and they were incubated for another 4 h. Protein degradation measured as the appearance of acid-soluble radioactivity was determined as described under "Experimental Procedures."
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Assay of Stimulated IL-2 Production in Jurkat Cells

Jurkat cells in 24-well plates at 7 × 106 cells in 1 ml of medium were treated with 0.1 µg of phorbol 12-myristate 13-acetate and 3.5 µg of ionomycin. After 4 h, the culture medium was collected and assayed for IL-2 using the Human IL-2 Quantikine kit (R&D Systems) according to the manufacturer's instructions.

Pulse-Chase of beta -[3H]Lactone in HeLa Cells

Four 15-cm plates of HeLa cells were grown to confluence and harvested to obtain 2.9 × 107 cells. The cells were suspended in 5 ml of medium, and 5 µl of 1.5 mM beta -[3H]lactone in acetonitrile was added (25 µCi of total 3H). The cells were incubated for 30 min, and 40 ml of ice-cold PBS was added. The cells were pelleted by centrifugation, the supernatant was removed, and the cells were resuspended in 6 ml of fresh medium. The cell suspension was aliquoted into microcentrifuge tubes at 4.8 × 106 cells/tube and pelleted by centrifugation. The supernatants were removed, and the cell pellets were resuspended in 500 µl of fresh medium. The tubes were incubated for the times indicated in Fig. 7A. At the indicated times, the cells were pelleted, and the supernatant was collected and acidified with 1 µl of concentrated trifluoroacetic acid. Tritium in the medium samples was determined by subjecting 50-µl aliquots to liquid scintillation counting. For HPLC analysis (e.g. Fig. 7B), aliquots of the medium were diluted 4:1 with water (0.06% trifluoroacetic acid) and filtered to remove particulate matter, and 180-µl aliquots of the filtered material were injected onto the column. Liquid scintillation counting of the samples before and after filtration showed that all of the tritium was recovered in the filtrate.


Fig. 7. Lactathione export from HeLa cells treated with beta -lactone. A, HeLa cells in suspension were incubated for 30 min with 1 µM beta -[3H]lactone, washed, and resuspended in fresh culture medium. At various times, the medium was collected, and the amount of tritium was measured by liquid scintillation counting (open circles). The closed circles represent lactathione accumulation as a function of time in HeLa cells treated with 50 µM unlabeled beta -lactone. Intracellular lactathione was determined by HPLC as described under "Experimental Procedures." B, an aliquot of the culture medium from 40 min into the chase period was subjected to reverse-phase HPLC, fractions were collected, and the tritium was determined by liquid scintillation counting. The UV chromatogram (lower trace) contains a small peak at 21.4 min that corresponds to 1 nmol of unlabeled lactathione that was added to the sample as an internal standard. The upper trace displays the tritium levels obtained in sequential fractions (1 min/fraction) of the HPLC eluate. The background for the liquid scintillation counter was 30-40 dpm.
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RESULTS AND DISCUSSION

Lactathione Formation In Vitro

-In aqueous solution, clasto-lactacystin beta -lactone readily reacts with N-acetyl-L-cysteine (NAC) to form the thioester adduct that is lactacystin (13). This prompted us to speculate that an analogous thioester adduct would form upon mixing beta -lactone with GSH. Fig. 1 shows the UV chromatograms of a reaction mixture containing beta -lactone and GSH along with controls for the two components incubated separately. Two new peaks were observed in the chromatogram of the reaction mixture. In addition, the peak corresponding to the beta -lactone had disappeared, but no peak corresponding to clasto-lactacystin dihydroxy acid was detected, indicating that the lactone had not hydrolyzed. The two new products were subsequently prepared in quantities sufficient for proton NMR and mass spectral analysis (data not shown). The larger of the two peaks (M + H = 521.8) corresponds to the predicted thioester adduct (Scheme 1), for which we suggest the trivial name lactathione. The smaller peak (M + H = 521.8) represents an isomer of lactathione, whose exact structure and origin are currently under investigation.

Aqueous Hydrolysis of Lactathione

In aqueous solution, lactacystin undergoes spontaneous hydrolysis to yield NAC and clasto-lactacystin dihydroxy acid. As shown in our previous work (13), this process proceeds exclusively through the intermediacy of the beta -lactone, and this latter species is solely responsible for proteasome inactivation. The hydrolysis of lactathione was studied employing the same methodology, and we obtained similar results as summarized in Scheme 1. Also, the inactivation of 20 S proteasome chymotrypsin-like activity by lactathione was examined as a function of GSH concentration as shown in Fig. 2. Increasing the GSH concentration, which will lower the steady-state level of beta -lactone (Scheme 1), also slows proteasome inactivation (Fig. 2). Likewise, at pH 6.5, where lactathione is stable because lactonization is suppressed (data not shown), the rate of proteasome inactivation by lactathione is greatly reduced (kinact/[I] <=  0.04 mM-1 s-1). Thus, the properties of lactathione in vitro are entirely analogous to lactacystin. Like lactacystin, lactathione does not function as a proteasome inhibitor, but it can serve as a source of the active species, clasto-lactacystin beta -lactone.


Fig. 2. GSH dependence of the rate constant for proteasome inactivation by lactathione. Inactivation of the chymotrypsin-like activity of the 20 S proteasome-PA28 activator complex by lactathione (2 µM) was assayed fluorometrically as described under "Experimental Procedures."
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Lactathione Formation in Cells

Of particular interest in Scheme 1 is the second-order rate constant for combination of beta -lactone with glutathione to form lactathione, 3.6 M-1 s-1, measured in PBS at 37 °C. This result suggested that in the physiological range of intracellular GSH ([GSH] >=  1 mM), intracellular beta -lactone would react rapidly (t1/2 <=  3 min) to form lactathione. Fig. 3 shows chromatographic analyses of extracts from Jurkat cells that had been exposed to beta -lactone, lactacystin, or vehicle (dimethyl sulfoxide). In the extracts from both beta -lactone- and lactacystin-treated cells, a peak with the same retention time as lactathione (21.4 min) was observed. This component is absent in the extract from vehicle-treated cells. The identity of this component as lactathione was confirmed in separate experiments by collecting it and subjecting it to mass spectral analysis. The major component that was detected had a mass identical to synthetic lactathione (see above). Interestingly, only lactathione, and not lactacystin, could be detected in the extracts from lactacystin-treated cells. Although the absence of lactacystin in extracts from lactacystin-treated cells is a negative result, control experiments suggest that it is significant. The retention time of lactacystin in this chromatographic system is 24 min, which is well resolved from any of the background peaks. Also, as a control for recovery, we supplemented extracts from naive cells with lactathione and lactacystin and then prepared the extracts for chromatography. The peaks corresponding to both lactathione and lactacystin were observed (data not shown), and both were obtained with 79% overall recovery through the procedure.


Fig. 3. Lactathione accumulation in Jurkat cells treated with beta -lactone or lactacystin. Jurkat cells were treated for 30 min with 20 µM beta -lactone (trace A), 20 µM lactacystin (trace B), or vehicle (0.1% dimethyl sulfoxide) (trace C). Extracts were prepared and subjected to reverse-phase HPLC as described under "Experimental Procedures."
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In light of these control experiments, we feel that our inability to detect lactacystin in extracts from lactacystin-treated cells suggests that either lactacystin does not enter these cells or that it enters the cells and is very rapidly converted to lactathione (e.g. the conversion might be catalyzed by a GSH transferase). To test these possibilities, we examined the effect of glutathione depletion on accumulation of lactathione inside the cells. A treatment of Jurkat cells with the gamma -glutamylcysteine synthase inhibitor, buthionine sulfoxime, that was sufficient to lower intracellular GSH concentrations by >95% had the expected effect of lowering lactathione accumulation in both beta -lactone- and lactacystin-treated cells (Table I). Nevertheless, no lactacystin could be detected by chromatographic analysis of the GSH-depleted, lactacystin-treated cells. Additionally, we tested the ability of a commercially available rat liver GSH transferase preparation to catalyze lactathione formation. The integrity of this enzyme preparation was confirmed by monitoring the conjugation of 1-chloro-2,4-dinitrobenzene in a standard assay (18). Nevertheless, no lactathione formation could be detected when lactacystin was tested as a substrate (data not shown). Also, lactacystin showed no ability to inhibit GSH transferase activity in the standard assay (data not shown).

Table I.

Glutathione depletion decreases lactathione accumulation in Jurkat cells treated with beta -lactone or lactacystin

Jurkat cells were incubated for 24 h in standard medium in the absence (control) or presence (GSH-depleted) of 1 mM buthionine sulfoxime. Total intracellular GSH was determined using the 5,5'-dithiobis(2-nitrobenzoic acid)-GSSG reductase recycling assay (17). Following glutathione depletion, cells were treated with either 20 µM beta -lactone or 20 µM lactacystin for 30 min. The cells were harvested and washed, and intracellular lactathione was determined as described under "Experimental Procedures."
Cells [GSH + GSSG] [Lactathione]
 beta -Lactone-treated Lactacystin-treated

nmol/5 × 107 cells nmol/5 × 107 cells
Control 220 2.3 0.51
GSH-depleted 5.9 0.36 0.09
GSH-depleted as % of control 2.7 16 18

We next examined the kinetics of lactathione accumulation in Jurkat cells treated with beta -lactone or lactacystin using the HPLC assay. Representative data are shown in Fig. 4A. Lactathione accumulation in beta -lactone-treated cells could be detected at the earliest time point that we were capable of obtaining. This time point is ~2 min, which is the time it takes to harvest and wash the cells after adding the drug. The intracellular lactathione concentration reaches a maximum at 15-30 min and then decays with a t1/2 of ~30 min. As a rough approximation, we can estimate the intracellular volume from the mass of the cell pellet assuming 1 mg/µl. 5 × 107 Jurkat cells have a mass of ~50 mg (i.e. 50-µl cell volume). Thus, the intracellular concentration of lactathione at the peak is ~140 µM, which is higher than the starting concentration (50 µM) of beta -lactone in the medium. We have also performed this experiment with HeLa cells, and the shape of the time course is nearly identical (data not shown). Nevertheless, the peak concentrations of lactathione observed in HeLa cells is lower by about two-thirds compared with Jurkat cells for the same dose of beta -lactone. This could reflect differences in the permeability of the drug in the different cell types; however, we suspect that this observation most likely reflects the condition that the HeLa cells are adherent cells and the Jurkat cells grow in suspension. On one occasion, we first harvested HeLa cells and then treated them with beta -lactone in suspension, and the lactathione accumulation was three times higher than when the cells were treated on the plates.


Fig. 4. Kinetics of lactathione accumulation in cells treated with beta -lactone or lactacystin. A, Jurkat cells were treated for various times with either 50 µM beta -lactone or 50 µM lactacystin. Extracts were prepared, and lactathione was measured by reverse-phase HPLC as described under "Experimental Procedures." B, shown is a hypothetical time course for the concentration of beta -lactone in aqueous medium starting with equal concentrations of either beta -lactone or lactacystin. The curves were calculated using the measured rate constants (see Ref. 1) for the lactonization of lactacystin (2.8 × 10-4 s-1) and the hydrolysis of beta -lactone (9.0 × 10-4 s-1) at pH 8 and 37 °C.
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The time course for lactathione accumulation in cells treated with lactacystin differed substantially from that with beta -lactone (Fig. 4A). The peak level was lower, and it was obtained at a longer time (45-60 min). Nevertheless, the decay was slower, so that at 60 min, the concentrations of lactathione in the lactacystin- and beta -lactone-treated cells are equal, and at longer times, the lactathione concentration is higher in the lactacystin-treated cells. The same observations were made in experiments with HeLa cells (data not shown). These results, together with consideration of the mechanism of lactacystin hydrolysis as elucidated in our previous work (13), suggested a model whereby only beta -lactone, but not lactacystin, can enter cells. To illustrate this point, we have performed a calculation of the time dependence of beta -lactone concentration in aqueous medium starting at zero time with equal concentrations of either beta -lactone or lactacystin (Fig. 4B). Starting with beta -lactone, the beta -lactone concentration decays in a first-order process due to hydrolysis. Stating with lactacystin, the beta -lactone concentration is initially zero and with time shows a transient accumulation. This represents the balance between production of beta -lactone via the lactonization of lactacystin and beta -lactone destruction via hydrolysis. The concentrations of lactacystin relevant to the cell culture experiments are relatively low (<= 50 µM), so that the NAC produced in the lactonization step never accumulates to a level that is high enough to drive the back-reaction (i.e. re-formation of lactacystin from beta -lactone and NAC) to an appreciable extent. Thus, one need only consider a simple model of two sequential first-order reactions for the calculation of beta -lactone concentration when starting with lactacystin. There are two crucial features of this system (Fig. 4B) that are relevant for consideration of the data (Fig. 4A). First, the concentration of beta -lactone will eventually be higher when starting with lactacystin. The timing of this (i.e. the time point at the intersection of the two curves) will depend on the relative magnitudes of the rate constants for lactonization and hydrolysis. Second, because the hydrolysis of lactacystin proceeds exclusively through the intermediacy of beta -lactone, the areas under the curves are equal. Both of these features parallel the lactathione accumulation data. First, we observed that at longer times (>= 60 min), lactathione concentration was higher in the lactacystin-treated cells. Second, the areas under the curves for the data in Fig. 4A (calculated by a model-independent approach (linear trapezoidal rule)) are 470 (nmol/105 cells) × min for the beta -lactone-treated cells and 410 (nmol/105 cells) × min for the lactacystin-treated cells. Thus, these kinetics suggest that a relationship of proportionality exists between intracellular lactathione accumulation and the extracellular concentration of beta -lactone. These results led us to propose the model of Scheme 2, where we hypothesize that lactonization of lactacystin occurs extracellularly and that beta -lactone is the only species that can enter the cells. Once inside, beta -lactone can reversibly react with GSH to form lactathione, or it can inactivate the proteasome.


Scheme 2. Mechanism of proteasome inhibition by lactacystin in cells.
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Proteasome Inhibition in Cells Treated with Lactacystin and beta -Lactone

The rapid hydrolysis of beta -lactone in aqueous solution (Scheme 1) is unavoidable in cell culture experiments. Nevertheless, this is offset to a large extent because of its rapid entry into cells (Fig. 4B). As a result, low micromolar concentrations of beta -lactone in medium can effect inhibition of the proteasome in cells. This is illustrated in Fig. 5A, which shows a dose-response curve for the inactivation of 20 S proteasome peptidase activity in Jurkat cells treated with beta -lactone. A 30-min incubation of the cells with >= 1 µM beta -lactone was sufficient to inactivate the proteasome peptidase activity by >= 90%. Inhibition of proteasome peptidase activity in cells can substantially suppress overall intracellular protein turnover (11). Fig. 5B shows dose-response curves for beta -lactone and lactacystin inhibiting the degradation of metabolically labeled "long-lived" proteins in C2C12 cells. Either compound was able to inhibit this turnover to a level of ~60%, and both showed similar potencies (IC50 approx  1 µM). Given the 5-10-fold lower activity that has been observed for lactacystin compared with beta -lactone in vitro (3, 13), these results were initially surprising. However, considering the different time scales for the cell culture experiments versus the in vitro assays of enzyme activity, the observation of similar potencies for the two compounds makes good sense. For example, the acid-soluble radioactivity that is used to monitor protein degradation in C2C12 cells (Fig. 5B) is accumulated over 4 h. In contrast, the assays with purified enzyme, as a matter of convenience, are designed so that the full extent of inactivation is obtained within a much shorter interval (<= 30 min). As illustrated in Fig. 4, at early times, the concentration of beta -lactone generated by lactonization of lactacystin is relatively low; however, given enough time, the cumulative exposure of cells to beta -lactone will be the same for both compounds.

The activation of the transcription factor NF-kappa B and, subsequently, the expression of the genes that NF-kappa B controls are dependent on the proteasome (12). The proteasome functions in this pathway by degrading Ikappa Balpha , an inhibitor of NF-kappa B that, under basal conditions, binds to NF-kappa B and sequesters it in the cytoplasm. The mechanism involves a signal-induced phosphorylation and ubiquitination of Ikappa Balpha , which targets this protein for degradation via the ubiquitin-proteasome pathway (19). Expression of the human IL-2 gene depends on NF-kappa B activation (20) and thus provides a useful system for studying a specific biological role of the proteasome and a specific consequence of proteasome inhibition (i.e. inhibition of IL-2 production). Fig. 6A shows the ability of lactacystin to suppress IL-2 production by stimulated Jurkat cells as a function of NAC concentration. The rationale for this experiment can be understood by reference to Scheme 2. Extracellular NAC will lower the extracellular beta -lactone concentration by driving the back-reaction to form lactacystin. Thus, unless lactacystin can enter the cells, the extracellular NAC should prevent proteasome inactivation. In the absence of NAC, 10 µM lactacystin applied 30 min prior to the stimulus decreased IL-2 production to ~10% of the control value (Fig. 6A). With increasing concentrations of NAC, IL-2 production was restored in the lactacystin-treated cells, reaching ~80% of the control value at 5 mM NAC. We also measured lactathione accumulation under these same conditions, and the results are presented in Fig. 6B. Addition of NAC suppressed intracellular lactathione accumulation in lactacystin-treated cells, and the dose response mirrors the restoration of IL-2 production (i.e. prevention of proteasome inhibition) in the cells. Independently of the effect of lactacystin, NAC suppressed IL-2 production by about one-third at 5 mM. This is most likely due to the well documented ability of NAC to prevent NF-kappa B activation (21), presumably reflecting the involvement of reactive oxygen intermediates in the signaling pathway and the ability of antioxidants such as NAC to buffer them (22).


Fig. 6. Effect of NAC on lactathione accumulation and induced IL-2 production in Jurkat cells treated with lactacystin. A, Jurkat cells were treated with 10 µM lactacystin (closed circles) or vehicle (0.1% dimethyl sulfoxide) (open circles) for 30 min in the presence of various concentrations of NAC. The cells were then stimulated with phorbol ester and calcium ionophore. At 4 h following the stimulus, IL-2 production was determined as described under "Experimental Procedures." B, Jurkat cells cultured in the presence of various concentrations of NAC were treated for 1 h with 50 µM lactacystin. Intracellular lactathione accumulation (closed circles) was determined as described under "Experimental Procedures." The data on IL-2 production in lactacystin-treated cells (A) were averaged and recalculated as percent of control for each NAC concentration and are replotted in B (open circles).
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Metabolic Fate of Lactathione

Inside cells, glutathione conjugation to xenobiotic electrophiles such as clasto-lactacystin beta -lactone is a common mechanism for detoxification of these compounds. In this aspect, the formation of lactathione upon entry of beta -lactone into cells may serve as a buffer against beta -lactone-mediated proteasome inactivation. Alternatively, the ability of lactathione to spontaneously regenerate the beta -lactone (via lactonization) suggests that lactathione could serve as a reservoir for the prolonged release of the active species. The rapid accumulation of lactathione in beta -lactone-treated cells (Fig. 4A) suggests that the cells are freely permeable to this molecule, whereas the observation that lactathione accumulates to a level that exceeds that of extracellular beta -lactone suggests that the cells are relatively impermeable to lactathione. Thus, lactathione formation concentrates beta -lactone inside the cell, albeit in an inactive form. However, this concentrating effect may be counterbalanced by subsequent metabolism of the lactathione. In vitro at physiological GSH concentrations (>= 1 mM), lactathione is very stable (t1/2 >=  15 h). In Jurkat or HeLa cells, lactathione disappears, with a half-time of ~30 min (e.g. Fig. 4A). This loss of lactathione from the cells may be mediated by a glutathione sulfur conjugate export pump in the cell membrane (23, 24, 25). Irrespective of the mechanism, it is clearly too fast to be accounted for by spontaneous lactonization of lactathione and subsequent hydrolysis of the beta -lactone. To examine the fate of intracellular lactathione, we prepared radiolabeled beta -lactone and performed pulse-chase experiments in HeLa cells. Fig. 7A shows the time course for the appearance of tritium in the medium from cells following a 30-min pulse of beta -[3H]lactone. These data are superimposed upon the time course for lactathione accumulation in HeLa cells treated with beta -lactone measured in a separate experiment. The appearance of tritium in the medium mirrored the loss of lactathione from inside the cells, suggesting that intracellular lactathione was transported out of the cells. To ascertain the identities of the tritiated species, aliquots of chase medium were subjected to reverse-phase HPLC, fractions were collected, and the column fractions were subjected to liquid scintillation counting (Fig. 7B). The major peak of radioactive material had a retention time coincident with lactathione (21-22 min). A second broad peak of radioactive material at an earlier retention time (8-11 min) may correspond to clasto-lactacystin dihydroxy acid (retention time = 8 min; see Fig. 1), which would be produced from lactonization and hydrolysis of lactathione during the chase interval. However, the peak fraction is at 10-11 min, which more closely corresponds to the cysteine sulfur adduct of the beta -lactone (i.e. desacetyllactacystin), which has a retention time of 10 min in this chromatographic system (data not shown). This species could potentially be generated via the sequential actions of gamma -glutamyl transpeptidase and dipeptidases upon export of lactathione from the cells (for review, see Ref. 26). Near the beginning of the chase interval, the major portion of the tritium was found associated with the lactathione peak (retention time = 24 min). At longer times, the major potion of the tritium was found associated with the earlier eluting peaks (retention time = 8-11 min). These kinetics suggest that lactathione is the predominant form being exported from the cells, with subsequent generation of the other species by either spontaneous chemical conversion (i.e. lactonization and hydrolysis) or enzyme-catalyzed reactions taking place outside the cells. A final point worth noting is that in vivo, the pathway for detoxification that is initiated by GSH conjugation is completed by uptake of the resulting cysteine sulfur conjugates into kidney tubules, where they are acted upon by N-acetyltransferases to produce mercapturic acids that are excreted in urine. Thus, the ultimate fate of lactacystin in vivo is predicted to be excretion of the mercapturate derived from lactathione, i.e. lactacystin.2


FOOTNOTES

*   The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Dagger    To whom correspondence should be addressed: ProScript, Inc., 38 Sidney St., Cambridge, MA 02139. Tel.: 617-374-1470; Fax: 617-374-1488; E-mail: ldick{at}proscript.com.
1    The abbreviations used are: HPLC, high performance liquid chromatography; FBS, fetal bovine serum; IL-2, interleukin-2; PBS, phosphate-buffered saline; NAC, N-acetyl-L-cysteine.
2    We call this hypothesis "Metabolic Irony."

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