Characterization of Inducible Nitric-oxide Synthase by Cytochrome P-450 Substrates and Inhibitors
INHIBITION BY CHLORZOXAZONE*

(Received for publication, October 1, 1996)

Stephan K. Grant Dagger , Barbara G. Green , Regina Wang , Stephen G. Pacholok and John W. Kozarich

From the Departments of Enzymology, Immunology & Inflammation, and Drug Metabolism, Merck Research Laboratories, Rahway, New Jersey 07065

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
Acknowledgments
REFERENCES


ABSTRACT

Nitric-oxide synthases (NOS, EC 1.14.13.39) are heme-containing enzymes that catalyze the formation of nitric oxide from L-Arg. General cytochrome P-450 inhibitors and cytochrome P-450 isoform-selective substrates and inhibitors were used to characterize the activity of recombinant human inducible NOS (iNOS). Classical cytochrome P-450 ligands such as the mechanism-based inactivator 1-aminobenzotriazole did not inhibit iNOS. Of a panel of 30 human cytochrome P-450 isoform-selective substrates and inhibitors, only chlorzoxazone, a cytochrome P-450 2E1 (CYP2E1) substrate, showed any significant inhibition of iNOS activity. Chlorzoxazone was not a substrate for iNOS but was a potent competitive inhibitor with respect to L-Arg with Ki = 3.3 ± 0.7 µM. The binding of chlorzoxazone to iNOS and human and rat liver microsomal cytochrome P-450 induced a high spin, type I spectra, which was reversed by imidazole. Although the binding of chlorzoxazone to iNOS and its inhibition of iNOS activity suggest some similarity between iNOS and CYP2E1 activity, other CYP2E1 substrates and inhibitors including zoxazolamine were not inhibitors of iNOS. Overall, iNOS activity is distinctly different from the major cytochrome P-450 enzymes in human liver microsomes.


INTRODUCTION

Nitric-oxide synthase (NOS, EC 1.14.13.39)1 belongs to a family of enzymes that catalyze the formation of nitric oxide (NO) and L-citrulline from L-Arg (for reviews see Refs. 1 and 78, 79, 80, 81). The reaction catalyzed by all the NOS isoforms is remarkable in its complexity by utilizing a host of substrates, cofactors, and regulatory factors, including L-Arg, NADPH, dioxygen, FAD, FMN, heme, tetrahydrobiopterin (BH4), Ca2+, and calmodulin. The NOS reaction mechanism has not been completely elucidated, but it is thought to proceed through two sequential cis-hydroxylations of one of the free guanidino-nitrogens of L-Arg. This is a five electron oxidation of L-Arg and requires 1.5 equivalents of NADPH and presumably 2 equivalents of O2 to form L-citrulline and the free radical gas, NO (2, 3, 4, 5).

Although the NOS isoforms catalyze the same reaction and require the same set of cosubstrates and cofactors, the primary sequences for the three mammalian enzymes, for example, are quite disparate except within defined regions associated with cofactor binding such as FAD, FMN, and NADPH. Two of the mammalian NOS isoforms are constitutively expressed, neuronal NOS (nNOS) and endothelial NOS (eNOS), and the third, inducible NOS (iNOS), is induced under cytokine stimulation within a large variety of cells. Nathan (6) and others (7, 8) have estimated that the primary sequences of the mammalian NOS isoforms average 53 ± 2% homology within a species. The conserved cofactor binding sites are contained within two separate structural domains of each subunit of homodimeric NOS (1, 2, 8). The N-terminal domain contains the oxygenase activity and binds heme, tetrahydrobiopterin, and the substrate L-Arg. The C-terminal domain has reductase activity (diaphorase) and binds FAD, FMN, and the nicotinamide cosubstrate. The calcium-dependent regulatory protein, calmodulin, binds within a region between the two domains and is thought to regulate electron transfer between the two NOS domains (9). For iNOS, calmodulin is tightly associated with the enzyme and represents a distinguishing feature for the inducible versus the constitutively expressed mammalian NOS isoforms (10, 11). The interaction between these two catalytic domains means that NOS isoforms have within a single protein the complete catalytic machinery of the two-protein microsomal electron transfer system, reminiscent of the bacterial fatty acid monooxygenase cyctochrome P-450BM-3 (12, 13).

The heme groups of all three mammalian NOS isoforms have been shown to be cytochrome P-450-type hemes by their CO difference spectra (14, 15, 16, 17, 18, 19, 20, 21). Unlike most cytochrome P-450s, however, the heme groups of these NOS isoforms are high spin, yet both iNOS and nNOS can bind imidazole to give low spin heme-imidazole complexes (18, 21). The application of resonance Raman scattering (22) and magnetic circular dichromism (23) spectroscopic techniques for studying the heme of rat nNOS further indicate that the heme environment for nNOS is distinct from other heme-containing enzymes such as cytochrome P-450CAM or fungal chloroperoxidase.

In order to further investigate the NOS heme domain and its binding specificity compared with that of other human P-450-containing enzymes, we examined a series of human cytochrome P-450 isoform selective and general substrates and inhibitors as potential inhibitors of recombinant human iNOS. We found that iNOS was poorly inhibited by compounds that are general cytochrome P-450 inhibitors, including 1-aminobenzotriazole. Isoform-specific cytochrome P-450 substrates and inhibitors were not recognized by iNOS, except for chlorzoxazone, a cytochrome P-450 2E1 (CYP2E1) substrate (24, 25). The mechanism of inhibition of iNOS by chlorzoxazone was examined as well as the binding of chlorzoxazone to human and rat hepatic microsomal P-450s.


MATERIALS AND METHODS

Reagents and Proteins

N-Tris[hydroxymethyl]methyl-2-aminoethane-sulfonic acid (TES), L-arginine hydrochloride, NADPH, FAD, FMN, bovine serum albumin, bovine calmodulin, acetaminophen (phenacetin), 1-aminobenzotriazole, DL-aminoglutethimide, benzphetamine hydrochloride, caffeine, dapsone, dextromethorphan hydrobromide, monohydrate, disulfiram (tetraethyl-thiuramdisulfide), enoxacin, erythromycin, hemin, ketoconazole, lubrol PX, metoxyphenamine hydrochloride, (+)-naringenin, nifedipine, perphenazine, tacrine, tolbutamide, and warfarin, sodium salt were purchased from Sigma. 7,8-Benzoflavone (alpha -naphthaflavone), chlorzoxazone (5-chloro-2-hydroxybenzoxazole), diethyldithiocarbamic acid, sodium salt, ellipticine, imidazole, 4-methylpyrazole, phenytoin (5,5-diphenylhydantoin), quinidine, and zoxazolamine were purchased from Aldrich. 6(R)-5,6,7,8-Tetrahydrobiopterin (BH4) was purchased from Dr. B. Schircks (Jona, Switzerland). L-[2,3,4,5-3H]Arginine hydrochloride (57 Ci/mmol) and L-[2,3,4,5-3H]citrulline (44 Ci/mmol) were purchased from Amersham Corp.. Troleandomycin was purchased from BIOMOL (Plymouth Meeting, PA). Debrisoquine sulfate, furafylline, 6-hydroxy-chlorzoxazone, and (±)-propranolol hydrochloride were purchased from Research Biochemicals International (Natick, MA). Benzylhydrazine hydrochloride and metyrapone were purchased from Fluka (Ronkonkoma, NY). Sulfaphenazole was a gift from Dr. T. Shibata (Meiji Yakuhin Co.). All other reagents were of the highest quality available.

Recombinant human NOS isoforms were cloned and expressed in the Sf9-baculovirus expression system according to a recent report (26). Purification of iNOS was performed by immunoaffinity chromatography as described elsewhere (27). Cell lysates containing recombinant human constitutive NOS isoforms (nNOS and eNOS) were desalted and used without further purification. Human liver microsomes (HLM-UM621: male, caucasian, no drug history, normal liver, protein content of 13.5 mg/ml, P-450 content of 0.3 nmol/mg) was a gift from Dr. Judy Raucy (Agouron Institute, La Jolla, CA). Rat liver microsomes were prepared as described previously (28).

Enzyme Assays

NOS activity was determined by a radiometric HPLC assay for L-[2,3,4,5-3H]citrulline produced from L-[2,3,4,5-3H]Arg as described previously (29, 30). A typical assay for L-[3H]citrulline production was carried out for 30 min at room temperature (22 °C) in 50 µl of assay buffer containing 0.1 M TES, pH 7.5, 1 µM L-Arg, 4 µCi/ml L-[2,3,4,5-3H]Arg, 5 µM FAD, 5 µM FMN, 0.5 mM NADPH, 0.2 mg/ml bovine serum albumin, 10 µg/ml calmodulin, 10 µM BH4, 0.25 mM dithiothreitol, 2 mM CaCl2, and 2-10 µg of enzyme. Enzyme reactions were quenched with 0.1 ml of stop buffer containing 0.2 mM sodium citrate, pH 2.2, 0.2 mM L-Arg, 0.2 mM L-ornithine, 0.2 mM L-citrulline, and 0.02% sodium azide. In preincubation experiments, the enzyme was added to assay buffer containing compound and 0.5 mM NADPH but without L-Arg for 15 min followed by the addition of L-Arg and assayed for 30 min. Conditions for the human hepatic CYP2E1 assay have been reported previously (31). Evaluation of the hydroxylation of chlorzoxazone (0.5 mM) by iNOS was performed under similar assay conditions for L-citrulline production (with 0.5 mM NADPH) but without L-Arg for 0, 0.3, 0.6, 1, 2, 3, and 5 h. Reactions (0.18 ml) were quenched with the addition of 20 µl of 85% H3PO4 and centrifuged to remove particulates. HPLC analysis of the reaction products of chlorzoxazone with iNOS or human hepatic microsomal P-450 was performed using a Zorbax SB-C8 (4.6 × 75 mm, 3.5 µm) reverse phase column with a gradient elution from 8 to 65% ammonium acetate in acetonitrile with 0.1% trifluoroacetic acid, 2 ml/min flow rate, and monitoring at 287 nm (30). HPLC retention times for the reaction products were confirmed by comparison with authentic chlorzoxazone and 6-hydroxy-chlorzoxazone as standards. Optical spectra were measured with a Hewlett Packard diode assay spectrophotometer (model HP8452A) equipped with a thermojacketed cell holder and circulating water bath. Spectra of iNOS (75 µg) were recorded at 15 °C in 0.3 ml of 20 mM TES, pH 7.4, 2 mM dithiothreitol, 10% glycerol, 1 mM 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane-sulfonate, 5 µM BH4, 2 µM FAD, FMN, and 0.2 M NaCl. Optical spectra of human liver (0.4 mg) and rat liver microsomal cytochrome P-450 (0.3 mg) and were recorded at 25 °C in 0.4 ml of 0.1 M KH2PO4, pH 7.4, 20% glycerol, and 0.2% lubrol PX. Optical spectra of hemin (6.7 µM) were recorded at 25 °C in 0.3 ml of 50 mM TES, pH 7.5. In order to minimize dilution of sample, additions of small volumes (0.3-1 µl) of concentrated imidazole and chlorzoxazone solutions were made to the sample cuvettes with a graduated 10-µl syringe.

Kinetic Analysis

The inhibitor dissociation constant, Ki, for chlorzoxazone was determined by fitting to the competitive inhibition equation: y = VMS/(S + Km(1 + I/Ki)), where y is initial velocity (pmol L-citrulline/min), S is L-Arg concentration, Km is the Michaelis constant, and I is chlorzoxazone concentration. IC50 and Ki determinations were made in either duplicate or triplicate and are given as average values with standard deviations. Apparent binding constants were determined from the difference spectra of the enzyme-inhibitor complex by fitting the change in absorbance to y = (aI)/(Kapp + I), where y is the change in absorbance, a is the maximum absorbance change, I is the inhibitor concentration, and Kapp is the apparent inhibitor dissociation constant. The equilibrium dissociation constant, Kd, for chlorzoxazone with iNOS was calculated after correction for the presence of imidazole, according to Kapp = Kd(1 + I/Kim) where I is the concentration of imidazole and Kim is the imidazole dissociation constant.


RESULTS

A series of general cytochrome P-450 inhibitors and isoform-selective cytochrome P-450 substrates and inhibitors were tested as potential inhibitors of recombinant human iNOS. The evaluation of general cytochrome P-450 inhibitors included reversible inhibitors and mechanism-based inactivators such as 1-aminobenzotriazole (32, 33). All of these compounds have been reported to inhibit or inactivate many cytochrome P-450s and therefore are not regarded as selective toward any single class of cytochrome P-450. In addition to the use of cytochrome P-450-specific antisera (34), the identification of a particular cytochrome P-450 activity can be determined by using a panel of compounds whose inhibitory activity or metabolism is associated with specific cytochrome P-450 isoforms, e.g. 1A1 or 2E1 (30, 35, 36, 37). We have examined both general cytochrome P-450 inhibitors and a series of cytochrome P-450 isoform selective substrates and inhibitors against iNOS in order to characterize its activity compared with human cytochrome P-450s. Each compound was tested at 0.1 mM against 1 µM L-Arg either competitively or by first preincubating the compound with iNOS for 15 min in the presence of NADPH. Preincubation of compounds with enzyme in the presence of NADPH was used to evaluated possible mechanism-based inactivation of iNOS.

Evaluation of General Cytochrome P-450 Inhibitors against iNOS

Human iNOS activity was not inhibited by any of the general cytochrome P-450 inhibitors tested at 0.1 mM (Table I) even after preincubation with the enzyme in the presence of iNOS cofactors and NADPH. 1-Aminobenzotriazole is a NADPH-dependent mechanism-based inactivator of many cytochrome P-450s and inactivates by alkylation of the heme group (38). This compound had no effect on iNOS activity. For hepatic microsomal cytochrome P-450s, the concentration of 0.1 mM 1-aminobenzotriazole was reported to be sufficient for the inactivation of enzyme activity (39). Its inhibitory activity is specific for cytochrome P-450-containing monooxygenases and does not affect other hemeproteins such as cytochrome b5 (40). Likewise, the mechanism-based cytochrome P-450 inactivator benzylhydrazine (41) had no detectable inhibition of iNOS. The P-450 ligands cyanide and azide were poor inhibitors of iNOS at 0.1 mM. iNOS activity was unaffected by NaN3 (up to 10 mM); however, KCN inhibited iNOS activity by greater than 75% at 5 mM. Apparently, iNOS binds low molecular weight anions very weakly. Together, these results demonstrate the uniqueness of iNOS activity compared with human cytochrome P-450 enzymes and most likely reflects the affinity of iNOS for cationic groups such as L-Arg rather than hydrophobic aromatics and anions.

Table I.

Effect of general cytochrome P-450 inhibitors against iNOS


Inhibitor Relative activity
PI = 0 mina PI = 15 minb

1-Aminobenzotriazole 1.00 1.02
DL-Aminoglutethimide 0.86 0.98
Benzylhydrazine 0.98 0.98
Metyrapone 1.11 0.97

a  Determined from relative values of L-[3H]citrulline formation at 0.1 mM compound against 1 µM L-Arg. Values shown are the average of duplicate determinations.
b  Assayed after 15 min of preincubation (PI) with enzyme in the presence of NADPH.

Evaluation of Cytochrome P-450 Isoform Selective Substrates and Inhibitors against iNOS

The identity of a particular hepatic cytochrome P-450 activity can be determined by the use of cytochrome P-450 isoform selective substrates and inhibitors (30, 35, 36, 37). Because these compounds are representative of substrates and inhibitors for the major human cytochrome P-450 families, they may be useful for determining if iNOS activity is similar to any human cytochrome P-450 activity. A panel of 30 compounds composed of human cytochrome P-450 isoform selective substrates and inhibitors were tested as inhibitors of iNOS. Table II summarizes the effect of these compounds against iNOS L-citrulline formation using 1 µM L-Arg. Only one compound, chlorzoxazone (5-chloro-2-hydroxybenzoxazole) (Fig. 1) showed any significant inhibition of iNOS. Chlorzoxazone is a substrate for CYP2E1 (24, 25), undergoing 6-hydroxylation, although some minor 6-hydroxylation has also been reported with cytochrome P-450 1A1 (42, 43, 44). However, two other CYP2E1 substrates, zoxazolamine, structurally similar to chlorzoxazone (see Fig. 1), and acetaminophen, did not inhibit iNOS. Moreover, the CYP2E1 inhibitors: disulfiram, 4-methylpyrazole, and diethyldithiocarbamic acid, were not inhibitors of iNOS. Whereas the inhibition of iNOS by chlorzoxazone suggests that iNOS may have similar substrate affinity as CYP2E1 (or cytochrome P-450 1A1), it is unlikely because none of the other CYP2E1 (or cytochrome P-450 1A1) selective compounds were inhibitors of iNOS. Also it is noteworthy that debrisoquine, which contains a guanidino-group, was not an inhibitor of iNOS even though debrisoquine can be hydroxylated to NG-hydroxy-debrisoquine by rat liver microsomal P-450 (45). Overall, the lack of inhibitory activity of general and isoform-selective cytochrome P-450 substrates and inhibitors toward iNOS demonstrates that iNOS substrate and inhibitor binding is unique from that of human cytochrome P-450 enzymes.

Table II.

Effect of cytochrome P-450-selective substrates and inhibitors against iNOS


Relative activitya

CYP1A1
  Inhibitors
    Ellipticine 0.94
    7,8-Benzoflavone 0.93
CYP2C8, CYP2C9, CYP2C18
  Substrates
    Benzphetamine 1.00
    Phenytoin 0.79
    Tolbutamide 1.00
    Warfarin 1.26
  Inhibitors
    Sulfaphenazole 0.97
CYP2E1
  Substrates
    Acetaminophen 1.01
    Chlorzoxazone 0.10
    Zoxazolamine 0.98
  Inhibitors
    DDCAb 0.99
    Disulfiram 0.96
    4-Methyl pyrazole 0.89
CYP1A2
  Substrates
    Caffeine 0.96
    Phenacetin 0.94
    Tacrine 0.97
  Inhibitors
    Enoxacin 1.01
    Furafylline 0.88
CYP2D6
  Substrates
    Debrisoquine 0.92
    Dextromethorphan 1.14
    Metoxyphenamine 0.88
    Perphenazine 0.74
    Propranolol 0.85
  Inhibitors
    Quinidine 0.89
CYP3A4
  Substrates
    Dapsone 0.92
    Erythromycin 0.93
    Nifedipine 0.89
  Inhibitors
    Ketoconazole 1.01
    (+)-Naringenin 1.05
    Troleandomycin 0.89

a  Determined in duplicate from relative values of L-[3H]citrulline formation at 0.1 mM compound against 1 µM L-Arg.
b  DDCA, diethyldithiocarbamic acid.


Fig. 1. Chemical structures of L-Arg, chlorzoxazone, and related compounds.
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Inhibition of iNOS by Chlorzoxazone

Chlorzoxazone was titrated against 1 µM L-Arg and inhibited iNOS L-citrulline production with an IC50 value of 6.9 ± 1.8 µM. The inhibition of iNOS by chlorzoxazone was competitive with respect to L-Arg with Ki = 3.3 ± 0.7 µM (Fig. 2). 6-Hydroxy-chlorzoxazone (see Fig. 1) was a less potent inhibitor of iNOS (relative activity = 0.52 @ 0.1 mM), but zoxazolamine did not inhibit iNOS even at 0.5 mM. Chlorzoxazone was also tested against recombinant human nNOS and eNOS as a measure of NOS isoform selectivity. IC50 values for the inhibition of nNOS and eNOS by chlorzoxazone were 12.9 ± 0.8 and 8.5 ± 0.5 µM, respectively, against 1 µM L-Arg. Chlorzoxazone inhibited all three human NOS isoforms with nearly equal potency.


Fig. 2. Competitive inhibition of iNOS by chlorzoxazone. Double recriprocal plot of initial velocity (pmol L-citrulline/min) versus L-Arg concentration at varying chlorzoxazone concentration (0.0 µM, circles; 1.0 µM, squares; 2.5 µM, triangles; 5.0 µM, inverted triangles; and 10.0 µM, diamonds). Kinetic parameters were determined from duplicate experiments (with a single experiment shown) by fitting to y = VMS/(S + Km(1 + I/Ki)), where Km = 1.7 ± 0.3 µM (L-Arg), VM = 0.50 ± 0.03 pmol L-citrulline/min, and Ki = 3.3 ± 0.7 µM.
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Although CYP2E1 catalyzes the 6-hydroxylation of chlorzoxazone, the likelihood of iNOS catalyzing the hydroxylation of a benzoxazole is remote because the substrate specificity of NOS isoforms is reported to be restricted to close L-Arg structural analogs such as L-homoarginine or NG-hydroxy-L-arginine (1, 3, 46). Although unlikely, this possibility was examined by incubating iNOS with chlorzoxazone under normal assay conditions with NADPH but without L-Arg. As a control we also incubated chlorzoxazone and NADPH with human hepatic microsomal P-450. The products were then analyzed by HPLC using authentic chlorzoxazone (6.4 min) and 6-hydroxy-chlorzoxazone (4.1 min) as standards. Fig. 3 shows representative HPLC traces for the products of chlorzoxazone with hepatic microsomal P-450 and iNOS. 6-Hydroxy-chlorzoxazone (4.1 min) was produced from chlorzoxazone (6.4 min) after incubation with hepatic microsomal P-450 (Fig. 3A). However, there was no detectable 6-hydroxy-chlorzoxazone or other products formed after 5 h of incubation of 0.5 mM chlorzoxazone with iNOS (at an enzyme concentration 10 times greater than for normal assays) (Fig. 3B). These results indicate that chlorzoxazone does not undergo any significant 6-hydroxylation by iNOS.


Fig. 3. HPLC analysis of reaction products. Human hepatic microsomal preparations and iNOS were incubated with 0.5 mM chlorzoxazone and 0.5 mM NADPH and other cofactors for up to 5 h at room temperature. Chlorzoxazone (6.4 min) was converted to 6-hydroxy-chlorzoxazone (4.1 min) by human hepatic cytochrome P-450 (A). Chlorzoxazone was unchanged after 5 h of incubation with iNOS (B) with no detectable formation of 6-hydroxy-chlorzoxazone. Inset, expanded section of chromatogram. Note: Peaks eluting before 3.0 min were also observed in control sample without iNOS. Chromatograms are representative of duplicate experiments.
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Reversible Type I Binding of Chlorzoxazone to iNOS

Because the inhibition of iNOS by chlorzoxazone is competitive with respect to L-Arg, chlorzoxazone is most likely binding within the heme domain. Perturbation of the heme optical spectra upon ligand binding has been used to characterize the mode of binding of substrates and inhibitors within the active sites of hemeproteins (47, 48, 49). The binding of various compounds to hemeproteins can induce low spin or high spin transitions of the heme iron, which accounts for the observed shifts in the heme optical spectra. For membrane-bound cytochrome P-450s the optical heme spectra can be complicated by the presence of detergents and other factors that can affect the spin state (49). Yet this method has been very useful for determining the binding of substrates and cofactors to soluble NOS isoforms (2, 18, 19). The iNOS substrate L-Arg and L-Arg analog inhibitors such as NG-methyl-L-arginine induce type I perturbations of the NOS heme spectra (18, 21, 50) and therefore are termed type I ligands. Type I ligands bind near the heme group but do not coordinate directly to the heme iron, and their binding induces a high spin heme-iron transition (48, 49). Zoxazolamine, for instance, induces a type I spectral shift of the optical spectra of rat hepatic microsomal P-450 (51). Based on the structural similarity of chlorzoxazone and zoxazolamine, we predicted that chlorzoxazone might also bind as a type I ligand to iNOS. However, the structure of chlorzoxazone is not very similar to L-Arg but more closely resembles the aromatic nitrogen-containing NOS inhibitors: 1-phenylimidazole (21, 52, 53, 54, 55) and 7-nitroindazole (56, 57). Imidazole and 1-phenylimidazole are type II ligands of iNOS, meaning that they bind as 6-axial ligands of the iron-heme group and induce a low spin heme-iron transition (21). These compounds have been reported as competitive inhibitors of iNOS with respect to L-Arg (21). The reversible binding spectra of 7-nitroindazole to iNOS has not yet been reported. Our attempts to measure the optical spectra of iNOS with 7-nitroindazole have been complicated by the strong absorbance of 7-nitroindazole, which interferes with the heme Soret band.2 In order to further characterize the nature of the binding of chlorzoxazone to iNOS, we measured the shift in the optical spectra induced by its addition to iNOS, hepatic cytochrome P-450s, and hemin.

We first examined the effect of increasing amounts of chlorzoxazone on the optical spectra of iNOS. This was performed by initially converting the native high spin heme of iNOS to low spin by the addition of the type II ligand, imidazole. The addition of chlorzoxazone then reversed the type II imidazole-induced spectra of iNOS, to give a type I difference spectra (lambda max at 394 nm and lambda min at 432 nm) (Fig. 4). A Kd value of 2.9 ± 0.2 µM was calculated for chlorzoxazone binding after the correction for the presence of imidazole. This value is virtually the same as the Ki = 3.3 ± 0.7 µM determined for the competitive inhibition of iNOS. Therefore, chlorzoxazone is a type I ligand of iNOS and most likely inhibits iNOS activity by binding competitively with respect to L-Arg within the heme domain at or near the L-Arg and imidazole binding sites.


Fig. 4. Type I difference spectra for iNOS with chlorzoxazone. Initially iNOS was converted to its low spin heme by the addition of 0.25 mM imidazole, a type II ligand (spectra not shown). The addition of increasing amounts of chlorzoxazone then reversed the imidazole-induced type II spectra of iNOS (75 µg, 15 °C) to give new absorbance maxima at 390 nm and minima at 430 nm with an isosbestic point at 412 nm. Representative difference spectra are shown: a-e, 5, 11.5, 16.4, 36, and 65.4 µM chlorzoxazone, respectively. Inset, reversible binding saturation curve for absorbance change (A390-430) versus chlorzoxazone concentration.
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Reversible Binding of Chlorzoxazone to Microsomal Cytochrome P-450

Because chlorzoxazone is a CYP2E1 substrate, we were also interested in determining its reversible mode of binding to human and rat liver microsomal cytochrome P-450s. Human and rat hepatic microsome preparations containing chlorzoxazone hydroxylating activity were analyzed by optical spectroscopy with chlorzoxazone and imidazole. Chlorzoxazone binding caused type I spectral changes for both human and rat liver microsomal cytochrome P-450s (Fig. 5). The addition of imidazole reversed the type I chlorzoxazone-induced spectra to give a type II spectra (lambda max at 432 nm and lambda min at 412 nm, spectra not shown). The addition of imidazole alone also gave a type II spectral shift with human and rat liver microsomal cytochrome P-450s (spectra not shown). Therefore, the binding of imidazole to either iNOS or microsomal cytochrome P-450s induces type II spectral changes and is exclusive with respect to the binding of chlorzoxazone.


Fig. 5. Type I difference spectra for hepatic microsomal cytochrome P-450 preparations with chlorzoxazone. A, addition of chlorzoxazone (1 mM) induced a type I spectral shift of human hepatic microsomal P-450 (1.0 mg/ml) with difference spectra absorbance maxima at 410 nm and minima at 424 nm and an isosbestic point at 418 nm. B, a type I spectra (difference spectra, A412-425) was also observed upon addition of chlorzoxazone (0.2 mM) to rat hepatic microsomal P-450 (0.8 mg/ml) with difference spectra absorbance maxima at 412 nm and minima at 425 nm with an isosbestic point at 420 nm.
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Reversible Binding of Imidazole and Chlorzoxazone to Hemin

Imidazole and other nitrogen-containing heterocycles interact with the heme iron through the ring nitrogen lone pair electrons (37, 59). It is this interaction for imidazole that induces the low spin heme iron transition with its characteristic type II optical spectra (37, 49). Structurally, chlorzoxazone might be expected also to bind as a type II ligand, but this is not what we observed for iNOS and the hepatic cytochrome P-450s examined here. This raises the question as to whether chlorzoxazone has an intrinsically weak bond with heme iron that makes its ligation undetectable by optical spectroscopy under these conditions. To evaluate this possibility we used chlorzoxazone and imidazole binding to hemin as a model for their binding to hemeproteins. As expected, imidazole caused a type II spectral change (lambda max at 432 nm and lambda min at 414 nm) upon addition to hemin with a very large Kd value of 8.7 ± 1.0 mM (data not shown). Chlorzoxazone alone also induced a type II spectral shift (lambda max at 438 nm and lambda min at 370 nm) upon binding to hemin with a Kd value of 0.26 ± 0.03 mM (Fig. 6). These dissociation constants may represent the binding of two molecules of either imidazole or chlorzoxazone to both sides of the porphyrin. This is different from what would be expected for iNOS and cytochrome P-450 enzymes where only a single molecule of chlorzoxazone or imidazole can bind axially because a thiolate ligand is also bound (16). These results demonstrate that chlorzoxazone can ligate with hemin to form a low spin heme iron complex. Therefore, although chlorzoxazone induces a low spin spectral shift for hemin, it binds specifically as a type I (high spin) ligand for iNOS and human and rat liver cytochrome P-450s.


Fig. 6. Type II difference spectra for hemin with chlorzoxazone. The addition of increasing amounts of chlorzoxazone to hemin (6.7 µM) induced a type II spectral shift with absorbance maxima at 438 nm and minima at 370 nm and an isosbestic point at 410 nm. Representative spectra are shown: a-e, 0.1, 0.2, 0.4, 1.0, and 2.0 mM chlorzoxazone, respectively. Insert, reversible binding saturation curve for absorbance change (A438-370) versus chlorzoxazone concentration.
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DISCUSSION

Human cytochrome P-450s have a conserved heme binding motif but overall have disparate primary sequences (36, 37, 60, 61, 62). Yet despite the sequence diversity among cytochrome P-450s, these enzymes still maintain the structural and catalytic requirements for NADPH-dependent hydroxylation of their substrates. This conservation of catalytic motifs for a family of enzymes with diverse primary sequences is even more dramatic for the NOS isoforms. Indeed, the cofactor requirements for NOS activity makes the NOS family of enzymes one of the most complex of the hemeproteins. Our results further demonstrate that iNOS activity is distinct from human cytochrome P-450 enzymes as evaluated by a panel of general and isoform-selective cytochrome P-450 substrates and inhibitors. Based on the results of 30 isoform-selective compounds, iNOS activity would appear to be more closely related to CYP2E1 activity, because: (i) chlorzoxazone (a CYP2E1 substrate) was the only compound from this collection identified as a potent, competitive inhibitor of iNOS and (ii) chlorzoxazone is a type I ligand of iNOS similar to that for human and rat hepatic microsomal P-450s. However, it can be equally argued that iNOS activity is not related to CYP2E1 because chlorzoxazone was the only CYP2E1-selective compound with inhibitory activity against iNOS. Several reports have also examined the effects of cytochrome P-450 substrates and inhibitors against eNOS and nNOS. The cytochrome P-450 substrate 7-ethoxyresorufin and inhibitors clotrimazole and octadecynoic acid had no inhibitory effect at 0.1 mM on eNOS activity in human umbilical vein endothelial cell lysates (63). There was some inhibitory activity reported for the cytochrome P-450 inhibitors miconazole, dihydroergotamine, and troeandomycin with bovine aortic eNOS (64). However, the reported inhibition was quite modest ranging between 26.3 and 44.0% inhibition for 0.05-0.10 mM inhibitor (64). Miconazole has also been tested as an inhibitor of nNOS along with ketoconazole and clotrimazole (65). Wolff et al. (65) reported that these three compounds were calmodulin antagonists and inhibited both bovine brain nNOS and calmodulin-dependent cyclic nucleotide phosphodiesterase. They concluded that these compounds were not competitive inhibitors of nNOS with respect to L-Arg, which is different from the reported inhibition of nNOS by imidazole (58, 65).

Several P-450 isoforms can catalyze reactions similar to the NOS-catalyzed oxidation of L-Arg. For example, debrisoquine (45) and 2-amino-5-chlorobenzophenone aminohydrazone (66) are hydroxylated by cytochrome P-450 2C3 to their N-hydroxyguanidine products. Rat hepatic microsomes can also catalyze the oxidation of NG-hydroxy-L-arginine to NO and L-citrulline and is inhibited by cytochrome P-450 3A4 inhibitors (67, 68). Although these examples have some similarities to the NOS-catalyzed reaction, they are notably different from the overall reaction, which proceeds without release of NG-hydroxy-L-arginine, except under specifically defined conditions and only at very low concentration (69). Perhaps the homodimeric nature of the NOS isoforms or the pterin cofactor are required for the sequential, uninterrupted cis-hydroxylation of L-Arg.

Chlorzoxazone is a skeletal muscle relaxant with a long history of use in humans (24, 70). Its pharmacokinetic profile has been studied extensively, and it is metabolized primarily by CYP2E1 (24, 25, 42, 43, 44). Several researchers have suggested that chlorzoxazone 6-hydroxylation may be a useful in vivo marker for hepatic CYP2E1 activity (24, 70) by following the pharmacokinetics of blood chlorzoxazone and 6-hydroxy-chlorzoxazone levels after a dose of 250 mg of chlorzoxazone (70, 71, 72). Our results with chlorzoxazone and human NOS isoforms may have some implications for the use of this compound as a marker for CYP2E1. Although variability was reported for the disposition of chlorzoxazone within populations of volunteers, mean maximum blood concentrations of 5.0 ± 1.9 µg/ml (30 µM) chlorzoxazone were measured 1-2 h after dosing (72, 73). At this blood concentration, chlorzoxazone might also inhibit NO biosynthesis. Given the multiple physiological roles of NO (1, 2, 8) as a neurotransmitter, vasodilator, and mediator in host defense, the use of chlorzoxazone should be exercised with caution in regard to its potential effects on NO biosynthesis.

There is a large pharmaceutical interest for the development of NOS inhibitors as potential therapeutics in a variety of human diseases including inflammation (74, 75) and cerebral ischemia (76, 77). The results presented here demonstrate that chlorzoxazone is a potent inhibitor of iNOS and to our knowledge is the first report of a benzoxazole class of NOS inhibitor. Given that chlorzoxazone is a potent NOS inhibitor and is considered to be a relatively safe drug with few side effects (24, 70, 71, 72, 73), it may have further utility in understanding the physiological and pathological roles for NO and may represent a novel structural class for the design of NOS isoform selective inhibitors.


FOOTNOTES

*   The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Dagger    To whom correspondence should be addressed: Merck Research Laboratories, P. O. Box 2000, Rahway, NJ 07065.
1    The abbreviations used are: NOS, nitric-oxide synthase; NO, nitric oxide; iNOS, inducible nitric-oxide synthase; eNOS, endothelial nitric-oxide synthase; nNOS, neuronal nitric-oxide synthase; CYP2E1, cytochrome P-450 2E1; BH4, 6(R)-5,6,7,8-tetrahydrobiopterin; TES, Tris[hydroxymethyl]methyl-2-aminoethane-sulfonic acid; HPLC, high pressure liquid chromatography.
2    S. K. Grant, unpublished results.

Acknowledgments

We are grateful to J. Calaycay, E. McCauley, S. Madhusudana, G. Wolfe, K. MacNaul, and Dr. N. Hutchinson for providing purified recombinant human iNOS and for nNOS and eNOS lysates. We also thank Dr. Debra Luffer-Atlas for advice concerning isoform-selective human cytochrome P-450 substrates and inhibitors and to Dr. Anthony Lu for critical reading of this manuscript.


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