(Received for publication, November 14, 1996, and in revised form, February 10, 1997)
From the Diabetes Research Laboratory, Massachusetts General
Hospital East, Charlestown, Massachusetts 02129, Mitotix, Inc., Cambridge, Massachusetts 02139, and the
§ Howard Hughes Medical Institute, Division of
Hematology/Oncology, Children's Hospital,
Boston, Massachusetts 02115
Antimitogenic stimuli such as environmental or
genotoxic stress, transforming growth factor-, and the inflammatory
cytokines tumor necrosis factor and interleukin-1 activate two
extracellular signal-regulated kinase (ERK)-based signaling pathways:
the stress-activated protein kinase (SAPK/JNK) pathway and the p38
pathway. Activated p38 phosphorylates transcription factors important
in the regulation of cell growth and apoptosis, including activating
transcription factor 2 (ATF2), Max, cAMP response element-binding
protein-homologous protein/growth arrest DNA damage 153 (CHDP/GADD153).
In turn, p38 lies downstream of the Rho family GTPases Cdc42Hs and
Rac1, as well as at least three mitogen-activated protein kinase
(MAPK)/ERK-kinases (MEKs): MAPK kinases-3, -6, and SAPK/ERK-kinase-1.
Although many of the stimuli that activate p38 can also inhibit cell
cycle progression, a clear-cut role for the p38 pathway in cell cycle
regulation has not been established. Using a quantitative
microinjection approach, we show here that Cdc42Hs, but not Rac1 or
RhoA, can inhibit cell cycle progression at G1/S
through a mechanism requiring activation of p38. These results suggest
a novel role for Cdc42Hs in cell cycle inhibition. Furthermore, these
results suggest that although both Cdc42Hs and Rac1 can activate p38
in situ, the effects of Cdc42Hs and Rac1 on cell cycle
progression are, in fact, quite distinct.
Protein kinase signal transduction pathways that employ members of the extracellular signal-regulated kinase (ERK)1/mitogen-activated protein kinase (MAPK) family have been remarkably conserved in evolution (1-3). Typically these pathways consist of a three-tiered core of protein kinases wherein MAPK-kinase-kinases (MAPKKKs) activate MAPK/ERK kinases (MEKs), which, in turn, activate ERKs (1-3). In simple eukaryotes, these pathways are activated by a variety of stresses, including nutrient withdrawal and osmotic shock (3). In mammals and other multicellular eukaryotes, ERK/MAPK pathways are activated by both environmental stresses and more complex physiologic stimuli such as mitogens, developmental cues, neurotransmitters, vasoactive peptides, and inflammatory cytokines (1, 2, 4).
The stress-activated protein kinases (SAPKs, also referred to as JNKs) and p38 are the ERK components in two mammalian signaling pathways activated by a broad array of environmental stresses as well as by inflammatory cytokines (1, 2). Once activated, the SAPKs and p38 are responsible for the phosphorylation and activation of transcription factors necessary for the stress response including c-Jun, ATF2, Max, and CHOP (1, 2, 5).
The signaling components upstream of the SAPKs and p38 which have been identified thus far suggest a complex cell- and stimulus-dependent regulation consistent with the diversity of extracellular stimuli that activate these pathways. Several chromatographically distinct MEKs, including SEK1/MKK4, SAPK-kinase (SAPKK)4, and SAPKK5 can activate the SAPKs in situ and in vitro. (6-8). SAPKK4 and SAPKK5 are specific for the SAPKs, whereas SEK1 can also activate p38. By contrast, MKK3 and MKK6 are specific p38 activators (8-10).
Four families of MAPKKKs have also been implicated in SAPK and p38
regulation. MEK kinases (MEKKs) 1 and 2, as well as the mixed lineage
kinase SH3 domain-containing, proline-rich kinase (SPRK), can activate
the SAPK pathway via direct activation of SEK1 (11-13). MEKK2 can also
activate the mitogenic MAPK/ERK1 and 2 pathway by directly
phosphorylating the ERK1/2 upstream activator MEK1 (11). Tpl-2 the rat
homolog of the human cot proto-oncogene product, like MEKK2,
can activate both SEK1 and MEK1 (14). MEKK3 can activate both the SAPK
and MAPK pathways, although SEK1 is not a MEKK3 substrate (11). In
addition, transforming growth factor--activated kinase-1 (TAK1) can
activate p38 in situ and has been implicated as a
transforming growth factor-
-activated MAPKKK upstream of SEK1, MKK3,
and MKK6 (15, 16). Finally, the p21-activated kinases (PAKs) and
germinal center kinase (GCK), mammalian homologs of Saccharomyces
cerevisiae STE20 and SPS1, respectively, can activate
the SAPK and p38 pathways, although their mechanisms of action are
unclear (17-20).
Genetic epistasis studies in yeast as well as biochemical and transfection experiments employing mammalian cells have identified members of the Ras superfamily as upstream elements regulating the core protein kinases in ERK signaling cascades (1, 2, 4, 19, 21-23). Thus, Ras is a critical upstream regulator of the Raf1 kinase, a MAPKKK in the MAPK pathway (4). Similarly, in mammalian cells, the Rho family GTPases Rac1 and Cdc42Hs can activate the p38 and SAPK pathways. This activation may involve the direct binding of Rac1 and Cdc42Hs to the PAKs. This binding results in PAK activation (18-20, 22-24).
A complete picture of the biological functions of these stress-activated pathways is only beginning to emerge. Recent data have implicated the SAPK and p38 pathways in the induction of apoptosis in response to nerve growth factor withdrawal in PC-12 cells, as well as heat shock, genotoxic chemotherapeutics (cis-platinum), and signaling pathways, which stimulate the generation of ceramide from membrane sphingomyelin (25-27). However, induction of apoptosis may not be the only biological consequence of activation of the SAPKs and p38. Moreover, the specific biological roles of the SAPK and p38 pathways in signaling mediated by Rac1 and Cdc42Hs have not been elucidated.
In this study, we used quantitative microinjection to begin to characterize the biological consequences of Cdc42Hs and Rac1 activation of p38. Here we report that Cdc42Hs is a potent inhibitor of cell cycle progression, arresting cells at the G1/S transition point. In addition, we demonstrate that this inhibition is mediated by elements of the p38 pathway. Rac1 and the SAPKs, although able to induce characteristic cellular and biochemical responses, do not inhibit cell cycle progression significantly.
NIH-3T3 cells were grown on glass coverslips
and synchronized in G0 by serum withdrawal for 24 h.
After this period, less than 1% of the cells incorporated
bromodeoxyuridine (BrdUrd) during an additional 24 h of
incubation. The arrested cells were released from serum starvation by
readdition of 10% calf serum and were microinjected in early
G1 with expression vectors harboring the HA-tagged or
FLAG-tagged cDNAs, using a Zeiss automated microinjection system
(28). As a control, cells were microinjected with the empty vector
together with an inert rabbit antibody marker to identify the injected
cells. S phase entry was monitored by BrdUrd (100 µM)
incorporation into cellular DNA (28, 29). Cells were fixed at the
indicated time after release from serum starvation as described. Cells
stained for phospho-p38 or phospho-c-Jun were fixed with 4%
paraformaldehyde according to the manufacturer's suggestions. Injected
DNA concentrations were: 100 ng/µl for HA-p38, HA-SAPK, HA-p44MAPK,
HA-p70S6 kinase, HA-MKK3, HA-MKK3-KR, HA-SEK1, HA-SEK1-KR, HA-MKK6,
M2-FLAG-GCK, and empty pMT3; 240 ng/µl for empty pMT3, HA-MKK3-KR, or
HA-SEK1-KR in the dominant negative experiments shown in Figs. 1, 3,
and 4; and 80 ng/µl for Rho family GTPases.
Immunocytochemistry
Fixed coverslips were stained with anti-HA mouse monoclonal antibody or anti-M2-FLAG mouse monoclonal antibody, followed by biotinylated anti-mouse antibody and streptavidin-Texas Red staining as described previously (28, 29). The coinjected rabbit IgG was detected with a biotinylated anti-rabbit antibody, followed by streptavidin-Texas-Red staining. The incorporated BrdUrd was detected by anti-BrdUrd rat monoclonal antibody and anti-rat fluorescein isothiocyanate antibody. Nuclei were counterstained with Hoechst 33258 dye (1 µg/ml). To quantitate cells in S phase, BrdUrd-positive cells were scored blindly as a percent of the number of injected cells expressing the tagged cDNAs. To stain for p38 following anti-HA staining, rabbit anti-p38 antibody was used followed by a 9-amino-6-chloro-2-methoxyacridine conjugated anti-rabbit antibody staining. The phosphorylated portion of the expressed HA-p38, the endogenous p38 protein, or c-Jun protein was detected by anti-phospho-p38 or anti-phospho-c-Jun antibodies, followed by biotinylated anti-rabbit antibody and streptavidin-9-amino-6-chloro-2-methoxyacridine or streptavidin-fluorescein isothiocyanate staining. Actin stress fibers were stained with fluorescein phalloidin as described (30). Membrane ruffling was detected by staining as described (31).
CloningMKK3 and MKK6 were cloned by polymerase chain reaction from human B cell (RAMOS) cDNA. p38, SAPK, MKK3, and SEK1 cDNAs were expressed from the pMT3 (HA-tagged) vector. GCK, MKK6, Cdc42Hs, Rac1 and RhoA plasmids were expressed from the pCMV5-M2-FLAG vector.
Murine NIH-3T3 fibroblasts were rendered quiescent by
serum withdrawal and synchronized in G1 by serum
readdition. Cells were microinjected in early G1 with
expression vectors encoding HA-tagged p38, SAPK-p46I, p44-MAPK, p70
S6 kinase, or empty vector. Entry into S phase was monitored by
incorporation of BrdUrd into DNA. After 24 h, expression of the
relevant constructs was verified by immunostaining, and the expressing
cells were scored blindly for S phase entry. Cells injected with the
empty vector were identified by staining for coinjected rabbit IgG.
Cells were also counterstained with bisbenzimide (Hoechst 33258) to
visualize all nuclei and assess any abnormal nuclear morphology. Fig.
1 shows representative photomicrographs of the stained
cells.
Expression of p38 resulted in a striking 77.5% inhibition of S phase
entry compared with cells injected with empty plasmid (Fig.
2a). Similar results were obtained using mink
lung Mv1Lu cells (data not shown). The inhibition of S phase entry by
p38 is likely due to the presence of increased amounts of active p38 in
the microinjected cells. Staining of the injected cells with an
antibody specific for the active, phosphorylated form of p38 (1, 2)
revealed that the p38-expressing cells contained greater amounts of
phosphorylated p38 than did the uninjected cells (Fig.
2b).
The ability of p38 to arrest cells in G1 was specific, with other signaling kinases having little or no effect on cell cycle progression. p44-MAPK is an integral component of the mitogenic pathway recruited by Ras, and constitutive activation of this pathway is sufficient to transform NIH-3T3 cells (4). Not surprisingly, expression of p44-MAPK modestly enhanced G1/S progression (91% of p44-MAPK-expressing cells in S phase versus 69% of empty plasmid-injected cells, Fig. 2a). p70 S6 kinase is also activated by mitogens through mechanisms involving phosphatidylinositol 3-kinase and FRAP/RAFT/TOR (32, 33). Inhibition of p70 S6 kinase blocks G1/S transition (34). Expression of p70 S6 kinase from microinjected plasmid had no significant effect on G1/S transition (Fig. 2a). SAPKs are preferentially activated by the same antimitogenic stimuli as is p38 (1, 2). To our surprise, however, expression of injected SAPK, which increased intracellular SAPK activity and, consequently, substantially elevated the level of phosphorylated c-Jun (a SAPK substrate) in the injected cells (Fig. 2b), resulted in only modest inhibition of S phase entry (55% in S phase; Fig. 2a). In view of this result, we explored the effects on cell cycle progression of strong activation of the SAPKs.
GCK is a mammalian homolog of S. cerevisiae SPS1 which, when expressed transiently, is constitutively active (1, 2, 17, 35). Coexpression of GCK and SAPK results in potent SAPK activation in the absence of external stimuli. At low levels of expression, GCK activation of SAPK is specific, with no p38 or ERK1 activation seen (17). At high levels of expression, however, GCK can activate p38 modestly.2 Injection of GCK into NIH-3T3 cells resulted in inhibition of G1/S transition to a degree commensurate with that obtained upon injection of p38 alone (25% in S phase versus 67% in S phase for cells injected with empty vector, Fig. 3). If KR-MKK3, a kinase-dead dominant inhibitory mutant of MKK3, which specifically blocks p38, but not SAPK activation (9 and see Fig. 4) is coinjected with GCK, this G1 arrest is abolished completely whether or not SAPK is coinjected with the GCK (Fig. 3). Thus, SAPK activation likely does not contribute to GCK-mediated cell cycle arrest.
Effects of MEKs Upstream of p38 on Cell Cycle ProgressionTo investigate further the role of activation of p38 in inhibition of G1/S transition, we asked if components known to lie upstream of p38 in stress signaling cascades could block S phase entry. MKK3, MKK6, and SEK1 are three mammalian MEKs that can activate p38 in situ and in vitro (9, 10). Although MKK3 and MKK6 are apparently specific for p38, SEK1 is also a strong SAPK activator (6, 9, 10). Expression of HA-tagged, wild-type SEK1, MKK3, or MKK6 from microinjected plasmids strongly inhibited S phase entry (14% in S phase for SEK1, 17% in S phase for MKK3, 15% in S phase for MKK6, 69% in S phase for empty plasmid). By contrast, expression of kinase-inactive mutant SEK1 or MKK3 plasmids, wherein the lysine residue critical for ATP binding has been mutated to arginine (KR mutants), was without significant effect (Fig. 4a).
Despite the lack of intrinsic activity, however, kinase-inactive SEK1 and MKK3 mutants can act as dominant inhibitors of coexpressed p38 activation and biological activity and can thus be used to assess the requirement for p38 activation for a particular biological function (6, 9, 10, 25-27). Accordingly, to test whether activation of p38 was necessary for p38-mediated cell cycle arrest, we coinjected p38 with KR-MKK3 or KR-SEK1. Consistent with a requirement for active p38, both KR-SEK1 and KR-MKK3 completely inhibited the ability of p38 to block S phase entry (Fig. 4b) without preventing p38 expression (Fig. 4c).
Effects of Rho Family GTPases on Cell Cycle ProgressionCdc42Hs, a member of the Rho subfamily of small
GTP-binding proteins, is a human homolog of S. cerevisiae
CDC42 (36). Cdc42p activates Ste20p as part of the yeast mating
pheromone signaling pathway. This pathway activates two ERKs, Fus3p and
Kss1p, and culminates in G1 cell cycle arrest (3, 21).
Likewise, GTP-charged Cdc42Hs or the related GTPase Rac1 can activate
the mammalian STE20 homologs PAK1 or PAK3 in situ
and in vitro; and recent evidence indicates that Cdc42Hs and
Rac1, but not RhoA, can activate p38 (and the SAPKs) in situ
via a mechanism requiring PAKs (18, 19, 22-24, 37). To investigate the
potential effects of Rho family GTPases on cell cycle arrest, we
microinjected expression plasmids encoding wild-type and mutant forms
of Cdc42Hs, Rac1 or RhoA into synchronized NIH-3T3 cells. Expression of
wt-Cdc42Hs resulted in a striking arrest of the cell cycle at
G1/S, exceeding that seen for p38. Of the cells injected
with empty plasmid, entry into S phase was apparent within 20 h
after release from serum starvation; and 75% of the cells injected
with empty plasmid had transited to S phase within 26 h. By
contrast, less than 7% of the cells expressing wt-Cdc42Hs entered S
phase within 30 h after serum readdition (Fig.
5a). This represents a dramatic 90.7%
decrease in S phase entry (Fig. 5, a and b).
Expression of wt-Cdc42Hs from injected plasmid also elevated the level
of activated, endogenous p38 phosphorylated at the sites critical for
activation, as determined by staining with an antibody specific for the
activated form of p38 (Fig. 5c).
Cdc42Hs, an upstream activator of p38, inhibits G1/S transition, whereas coexpression of Cdc42Hs with KR-MKK3, KR-SEK1 blocks Cdc42Hs-mediated cell cycle inhibition. Part a, time course of cell cycle progression in cells injected with the expression plasmids indicated. Part b, quantitation of S phase cells 26 h after release from serum starvation. Shown are the mean ± S.D. For each injection set the ratios of S phase expressing cells/total expressing cells were: empty vector: 29/37, 98/157, 130/172, 108/143, 150/197; wt-Cdc42Hs: 9/96, 2/25; 3/62, 1/45, 15/129, 14/145; wt-Cdc42Hs plus KR-MKK3 and KR-SEK1: 45/102, 24/68, 12/35, 26/80; V12-Cdc42Hs: 11/42, 18/50, 11/49; N17-Cdc42Hs: 7/34, 6/25, 10/35, 8/34. Part c, expression of wt-Cdc42Hs increases endogenous phosphorylation of p38 at the regulatory sites, whereas expression of N17-Cdc42Hs does not. Expression of the injected wt- and N17-Cdc42 cDNAs was verified with anti-FLAG staining (panels A and C, respectively). Staining for phospho-p38 is shown in panels B and D. Arrows indicate nuclear phospho-p38 staining (panel B) or mark the nuclei of the injected cells (panel D). Part d, expression of KR-SEK and KR-MKK3 has no effect on expression of coinjected Cdc42Hs. The expressed proteins (in red) were stained with anti-FLAG (panels A and B), and the rabbit IgG coinjected with the empty vectors were stained with anti-rabbit antibody (panel C). Nuclei of cells in S phase are stained with anti-BrdUrd and shown in green (panels A-C). All nuclei were stained with Hoechst 33258 (panels D-F). Injected cDNAs were: wt-Cdc42Hs (panels A and D), wt-Cdc42Hs plus KR-MKK3 and KR-SEK1 (panels B and F), empty vectors plus rabbit IgG (panels C and F).
Coexpression of dominant interfering (KR) SEK1 and MKK3, although having no effect on Cdc42Hs expression (Fig. 5d), resulted in a partial reversal of the cell cycle inhibitory effects of Cdc42Hs with 37% of the cells progressing to S phase, 44% of control (Fig. 5b). These results suggest that p38 activation is a necessary component in the mechanism by which Cdc42Hs inhibits cell cycle progression.
To investigate further the role of Cdc42Hs in G1 arrest, we next sought to determine the effects of constitutively active and dominant interfering mutants of Cdc42Hs on cell cycle progression. V12-Cdc42Hs is a GTPase-defective mutant of Cdc42 which is constitutively active. Microinjection of V12-Cdc42Hs, although still strongly able to arrest cells in G1, is less effective than wild-type (Fig. 5b). We attribute this to the possible nonspecific activation of mitogenic pathways by the activated Cdc42Hs mutant. N17-Cdc42Hs is a dominant interfering mutant of Cdc42Hs which is thought to act by sequestering nucleotide exchangers for Cdc42Hs and other Rho family GTPases (38). Thus N17-Cdc42Hs can act either by blocking Cdc42Hs, Rac1 or RhoA activation (38). Consistent with this, microinjection of N17-Cdc42Hs does not activate endogenous p38, as detected by staining with antibodies to phospho-p38 (Fig. 5c). However, to our surprise, N17-Cdc42Hs inhibited G1/S progression nearly one-third as effectively as wt-Cdc42Hs (Fig. 5b). This result supports the contention that the N17 mutant prevents activation of other Rho family GTPases, such as Rac1, which are required for cell growth.
It is conceivable that injection of wt-Cdc42Hs could nonspecifically
recruit other Rho family GTPases that inhibit cell cycle progression.
To investigate the specificity of cell cycle inhibition by Cdc42Hs, we
assayed for cell cycle inhibition mediated by other members of the Rho
family. To our surprise, injection of either a wild-type or
constitutively active allele of Rac1 (V12-Rac1), either of which can
activate p38 in situ (19), was far less effective at
inhibition of G1/S transition than was wt-Cdc42Hs (50% in
S phase for both Rac1 constructs versus 67% in S phase for
cells injected with empty vector; Fig. 6a),
even though injection of either wt- or V12-Rac1 was sufficient to
activate p38 to an extent commensurate with that induced by Cdc42Hs, as
judged by staining for activated endogenous p38 with anti-phospho-p38
antibodies (Fig. 6b). Moreover, injection of V12-Rac1
stimulated membrane ruffling, a characteristic response of the cell to
activation of Rac1 (31) (Fig. 6c). This result is consistent
with earlier observations indicating that Rac1 is in fact a necessary
component for Ras transformation (39). Thus, although Rac and Cdc42Hs can both activate p38 in situ (18, 19, 23), each may also activate additional pathways that together result in distinct biological effects. RhoA has not been shown to lie upstream of p38 or
the SAPKs (22, 23). Injection of a constitutively active (V14) RhoA
allele, although stimulating the characteristic formation of actin
stress fibers (30) (Fig. 6c), yielded cell cycle results similar to those obtained upon injection of Rac1 (49% in S phase, Fig.
6a). Thus, among Rho family GTPases, cell cycle inhibition appears to be specific to Cdc42Hs.
p38 and the SAPKs are activated by an array of ligands that are
known to be either antimitogenic or proapoptotic (1, 2). In contrast to
the mitogenic pathways, which are regulated largely by polypeptide
ligands coupled to tyrosine kinases, the stimuli that activate the
stress pathways are remarkably diverse and include ionizing radiation,
heat shock, chemical DNA damage, oxidative stress, reperfusion injury,
and the inflammatory cytokines tumor necrosis factor- and
interleukin-1. Thus, the upstream molecular components that feed into
the p38 and SAPK pathways are accordingly complex and diverse. The Rho
family GTPases Cdc42Hs and Rac1 represent two distinct mechanisms of
p38 and SAPK activation with different biological functions.
We have demonstrated herein that activation of Cdc42Hs inhibits cell
cycle progression at G1/S. Insofar as Cdc42Hs (and p38) inhibition of cell cycle progression can be at least partially reversed
upon expression of kinase-dead MKK3 and SEK1, we conclude that
recruitment of p38 is a critical component of Cdc42Hs-mediated cell
cycle arrest. In addition, we have shown that p38 or elements immediately upstream of p38 (SEK1, MKK3, or MKK6) can also arrest cells
in G1. By contrast, Rac1, which can also activate p38 (19, 23), fails to inhibit strongly cell cycle progression. Indeed, Rac1 has
been implicated in Ras transformation (39) and may recruit additional
signaling pathways that prevent inhibition of the cell cycle or promote
cell cycle progression. Thus, although activation of p38 alone is
sufficient to arrest cells in G1, we cannot rule out that
p38, which can be activated weakly by mitogenic signals, could, in
conjunction with other signaling pathways, lead to responses distinct
from G1 arrest. These ideas are summarized in the model
shown in Fig. 7.
Our results are in contrast with data implicating Rho family GTPases, notably Cdc42Hs, in cell growth and transformation (40). It is conceivable that the concomitant recruitment of Ras and several Rho family GTPase signaling pathways could result in cell growth, whereas activation of Cdc42Hs alone or in conjunction with other antimitogenic pathways results in cell cycle arrest. In this regard, care must be taken in the interpretation of results concerning Cdc42Hs mutants. Our results presented in Fig. 5 suggest that wt-Cdc42Hs is the most potent inhibitor of cell cycle progression, whereas a constitutively active mutant (V12-Cdc42Hs), although still growth inhibitory, is less so than wild-type. This result may reflect the ability of the overexpressed, constitutively active Cdc42Hs mutant to recruit nonspecifically mitogenic signaling mechanisms, possibly including those normally regulated by other Rho family GTPases, more effectively than the overexpressed wild-type Cdc42Hs. By the same token, overexpression of dominant negative Cdc42Hs, although unable to activate endogenous p38, was growth inhibitory, possibly because of nonspecific sequestering of the activation machinery for other Rho family GTPases. For example, Ost, a proto-oncogene, is a guanine nucleotide exchanger for Rho, Cdc42Hs, and Rac1 (38).
We do not believe that Cdc42Hs inhibition of G1/S transition is the result of nonspecific recruitment of p38 inasmuch as wild-type p38 itself, as well as MKK3, MKK6, and SEK1, all p38 activators, are also able to arrest NIH-3T3 cells at G1/S. Moreover, recent evidence indicates that p38 overexpression inhibits mitogen induction of G1 cyclins (41). Thus it is not surprising that p38 activation, by a Cdc42Hs-dependent mechanism, could result in growth arrest. Moreover, precedent for Cdc42Hs as a G1 inhibitor has been observed in lower eukaryotes. Thus, S. cerevisiae Cdc42p is an essential component of the mating pheromone response pathway, which also results in G1 arrest (3, 21). Inasmuch as the experiments implicating Cdc42Hs in cell growth have employed Swiss 3T3 cells (40), our results with NIH-3T3 and Mv1Lu cells may indicate that the growth-promoting effects of Cdc42Hs in Swiss 3T3 cells are cell-specific.
The reasons for G1 arrest in response to stress signals have not been clearly defined; however, it is plausible to propose that cell cycle arrest would be followed by repair of stress-related damage. Alternatively, the cell could arrest in G1 and await restoration of a normal cellular milieu conducive to continued growth. The targets of Cdc42Hs and p38 which mediate cell cycle arrest are not known. However, inhibition of induction of G1 cyclins (41) is a logical candidate. Moreover, CHOP, a p38 substrate, promotes G1 arrest as part of the response to genotoxic stress (5, 42).
Finally, several recent studies indicate that recruitment of the SAPKs or p38 results in apoptosis. Withdrawal of nerve growth factor from PC-12 cells activates both the SAPKs and p38 and promotes apoptosis. Expression of KR-MKK3 or KR-SEK1 blocks this effect, and constitutively active mutants of MKK3 or SEK1 promote apoptosis in the presence of nerve growth factor (25). Likewise, thermotolerant fibroblasts display defective SAPK activation and cell death in response to heat shock. Expression of SEK1 restores heat sensitivity to these cells (26). Finally, treatment of macrophage cell lines with agonists that stimulate sphingomyelin hydrolysis (tumor necrosis factor, UV radiation, x-irradiation, oxidant stress) results in apoptosis, a response that can be reversed upon expression of dominant inhibitory SEK1 (27). Despite these findings, in none of the experiments shown in Figs. 1, 2, 3, 4, 5, 6 did we see evidence of apoptosis. Thus, cycle arrest at G1/S, rather than apoptosis, appears to be the characteristic response of NIH-3T3 cells to activation of the Cdc42Hs/p38 pathway, indicating that different cells respond distinctly to p38 activation.
We thank J. Settleman for Rac, Rho, and Cdc42Hs cDNAs; J. H. Kehrl for GCK cDNA; A. Nebreda for anti-p38 antiserum; J. Avruch for p70 S6 kinase cDNA and for a critical reading of the manuscript; D. Ron for CHOP cDNA; M. Pagano, R. Bruns, and S. Brill for advice; the Massachusetts General Hospital Molecular Oncology Laboratory for use of their microinjection apparatus; and the Cutaneous Biology Research Center for the use of their microscope facilities. Á. M. thanks Á. Molnár, Sr. for continuous support.