COMMUNICATION:
Cystic Fibrosis Transmembrane Conductance Regulator Inverts Protein Kinase A-mediated Regulation of Epithelial Sodium Channel Single Channel Kinetics*

(Received for publication, January 2, 1997, and in revised form, April 8, 1997)

M. Jackson Stutts Dagger , Bernard C. Rossier § and Richard C. Boucher

From the Department of Medicine, Cystic Fibrosis/Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill, North Carolina 27599-7248 and the § Universite de Lausanne, Institut de Pharmacologie et de Toxicologie, 27 Rue du Bugnon, CH-1005 Lausanne, Switzerland

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
REFERENCES


ABSTRACT

Abnormal regulation of ion channels by members of the ABC transport protein superfamily has been implicated in hyperinsulinemic hypoglycemia and in excessive Na+ absorption by airway epithelia in cystic fibrosis (CF). How ABC proteins regulate ion conductances is unknown, but must generally involve either the number or activity of specific ion channels. Here we report that the cystic fibrosis transmembrane conductance regulator (CFTR), which is defective in CF, reverses the regulation of the activity of single epithelial sodium channels (ENaC) by cAMP. ENaC expressed alone in fibroblasts responded to activation of cAMP-dependent protein kinase with increased open probability (Po) and mean open time, whereas ENaC co-expressed with CFTR exhibited decreased Po and mean open time under conditions optimal for PKA-mediated protein phosphorylation. Thus, CFTR regulates ENaC at the level of single channel gating, by switching the response of single channel Po to cAMP from an increase to a decrease.


INTRODUCTION

Recent studies (1, 2) have identified ENaC as the channel that mediates amiloride sensitive Na+ absorption in mammalian airways. In cystic fibrosis (CF),1 ENaC-mediated Na+ absorption is increased 200-300% in airway epithelia and, abnormally, further stimulated by raising intracellular cAMP (3). Because most CF mutations result in little if any functional CFTR in the apical cell membrane of affected epithelia (4), we inferred that normal CFTR must either down-regulate the number of active Na+ channels or decrease the activity of individual Na+ channels. In the present study we have studied the effects of cAMP-dependent protein-phosphorylating conditions on the single channel kinetics of ENaC expressed alone or together with CFTR in NIH 3T3 fibroblasts.


EXPERIMENTAL PROCEDURES

alpha -, beta -, and gamma -ENaC subunits were stably expressed in NIH 3T3 cell lines that had been previously transduced with a truncated (inactive) interleukin-2 receptor (ENaC alone cells) or with human CFTR (ENaC + CFTR cells) (5). ENaC-mediated single channel currents were recorded from cell attached and excised membrane patches as described in the figure legends.


RESULTS

The single channel conductance (4-5 picosiemens) of ENaC expressed in NIH 3T3 fibroblasts, as well as cation selectivity (Li+ > Na+ > K+), amiloride inhibition (Ki approx  0.3 µM) and the slow gating pattern (MOT approx  1 s), are similar to what has been reported for the cloned channel expressed in oocytes (6, 7) and for endogenously expressed ENaC in rat cortical collecting tubule (8) or A6 cells (9) (Fig. 1). These similar results in very different cells suggest that cell specific cytoskeletal or other elements are not critical determinants of the basic biophysical characteristics of ENaC. The basal conductance and amiloride sensitivity of ENaC were not affected by co-expression with CFTR (Fig. 1).


Fig. 1. Characteristics of ENaC expressed heterologously in NIH 3T3 fibroblasts. A, representative pipette currents at a range of clamp voltages from a patch excised from an ENaC-expressing cell. B, IV plots, including the data from A, and similar data obtained from patches excised from cells expressing ENaC alone (bullet ) and cells expressing CFTR and ENaC (open circle ). Clamp voltage is plotted as -Vpipette. Downward (inward) currents represent cations leaving the pipette. C, effect of amiloride on ENaC contained in an outside out patch. D, amiloride concentration versus inhibition of initial Po (bullet  and open circle  as above). The approximate ED50 was 0.3 µM. Methods: NIH 3T3 fibroblasts that were previously stably transduced with CFTR or the inactivated interleukin-2 receptor (26) were infected with a tricistronic retrovirus containing alpha -, beta -, and gamma -rENaC cDNAs as reported (5) and maintained in Dulbecco's minimal essential medium-H supplemented with 10% fetal bovine serum, 1 µM amiloride, 100 units/ml penicillin, 0.1 mg/ml streptomycin, and 250 µg/ml G418 at 37 °C in an atmosphere of 95% air and 5% CO2. Cells were plated for patch clamp experiments on 35-mm culture dishes and incubated for 24 h in growth medium containing 2 mM sodium butyrate to stimulate ENaC expression by the long terminal repeat promoter, washed thrice with bath solution containing (in mM) 150 Tris aspartate, 2 MgSO4, 1 calcium gluconate, and 5 TES (buffered to pH 7.2 with CsOH), and studied on the stage of an inverted microscope at 22 °C using standard patch clamp configurations (27). Pipette solution, unless noted, was 280 lithium aspartate, 2 MgSO4, 1 calcium gluconate, and 5 TES. Current was recorded on VCR tape (Vetter Instruments). Selected recorded currents were filtered (50 Hz, Ithaco) and digitized (1000 Hz, Labmaster 1200) for analysis using PClamp 6.0 Software (Axon Instruments). Single channel amplitude at each voltage was determined by the separation of peaks in amplitude histograms or by the mean of at least 12 openings measured with the cursor of a digital oscilloscope. The means of two to three separate experiments are plotted in B. Selectivity was demonstrated by lack of reversed currents against Tris aspartate or potassium aspartate in the bath with 280 mM lithium aspartate (B) or 140 mM lithium aspartate or sodium aspartate in the pipette (not shown). Amiloride was exposed to outside out patches while recording active channels by diluting the bath by 1/2 with 200% of the desired final concentration. Po was calculated as the ratio of area under open peaks to total area of amplitude histograms.
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ENaC present in excised membrane patches exhibited a variable degree of rundown following excision. Rundown was partially reversed (Fig. 2A, panel i) or prevented (Fig. 2A, panel ii) by exposure of the cytoplasmic surface to PKA catalytic subunit and 2 mM ATP (CS + ATP). Fig. 2A, panel iii, summarizes the results from both paradigms, revealing positive regulation of ENaC activity by PKA. One explanation for a range of basal activity, for rundown following excision, and for variable degree of activation by CS + ATP is that the resting phosphorylation state differs from patch to patch. Moreover, it seemed possible that water-soluble reagents, such as PKA catalytic subunit, might have poor access to hydrophobic compartments within the membrane patch. We tested these possibilities with a specific peptide inhibitor of PKA (mPKI) that had been modified by myristoylation to promote its association with biologic membranes (10, 11). mPKI was effective in (6/6) inside out membrane patches, reversing the effects of exogenous CS + ATP (Fig. 2A) by inhibiting Po (Fig. 2A, panel iii) and MOT (not shown) to levels lower than "basal." This observation suggests that the level of basal phosphorylation in the system influences the gating of ENaC in the absence of external manipulation.


Fig. 2. Effect of CFTR on regulation of ENaC by PKA in excised patches. A: panel i, current recorded from an inside out patch of ENaC only cell, starting just after excision. "c" indicates all channels closed. The probability of one channel being open decreased from 0.72 in the first 60 s following excision to 0.42 in the 60 s before addition of CS + ATP (rundown) and increased during exposure to CS + ATP to 0.65 in the last 60 s before addition of mPKI. mPKI completely inhibited ENaC. Panel i is representative of six experiments carried out with this paradigm). Panel ii, experiment illustrating the excision of an ENaC only cell attached patch directly into bath solution containing CS + ATP. Up to six ENaC remain active until exposed to mPKI by addition to the bath. Panel ii is representative of five patches excised into CS + ATP. Panel iii, summary of Po calculated from data recorded (minimum duration of 60 s) from inside out patches exposed to different bath solutions. Basal (n = 11) includes the six patches from panel i and five patches studied under basal conditions only; CS + ATP (n = 11) includes all patches from panels i and ii; and mPKI (n = 6) includes five patches from panel i and one patch from panel ii. *, different from basal by unpaired t test, p < 0.05). **, different from CS + ATP by unpaired t test, p < 0.01). B: panel i, similar experiment as in A (panel i) but paradigm carried out on a patch excised from a ENaC + CFTR cell. Panel ii, effect of excision into CS + ATP on ENaC in a patch made from an ENaC + CFTR cell. Panel iii, summary of Po of ENaC + CFTR patches, as described for A, panel ii. Basal, n = 10, CS + ATP (n = 10), mPKI (n = 5). Methods: membrane patches were excised in the inside out mode. Basal refers to stationary channel activity following excision or just before exposure to CS and ATP. "CS + ATP" refers to the highest Po observed during a minimal interval of 60 s in the period 3-10 min following exposure to 100 units/ml CS (Promega) + 2 mM ATP to the bath. mPKI refers to the Po recorded in the period from 15 to 75 s following exposure to 1 µM mPKI (Biolmol) in the bath. Po was determined from amplitude histograms. For multichannel patches, nPo was calculated and Po derived assuming independent and equal gating of each channel and observation of maximal number of channels in the patch during recording.
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The presence of CFTR caused a dramatic change in the regulation of ENaC in excised patches by CS + ATP. Whereas the gating and rundown of ENaC in patches excised from CFTR expressing cells were not obviously abnormal under nonstimulated conditions, exposure to CS + ATP routinely inhibited ENaC activity in two different paradigms (Fig. 2B). First, in 4/5 excised inside out patches, CS + ATP decreased Po (Fig. 2B, panel i). Second, ENaC in 5/5 patches excised from CFTR expressing cells directly into CS + ATP demonstrated low Po (Fig. 2B, panel ii) and MOT (not shown). mPKI further decreased Po of ENaC co-expressed with CFTR (Fig. 2B, panels i and iii). Fig. 2B, panel iii, summarizes the very different pattern of regulation of ENaC by PKA in the presence of CFTR (compare with Fig. 2A, panel iii).

To study PKA and CFTR regulation of ENaC in the absence of excision-induced rundown, we exposed cells to permeant PKA activators (cpt-cAMP + forskolin (cpt-cAMP/FSK)) during cell-attached recording (Fig. 3). In ENaC-only cells cpt-cAMP + forskolin increased ENaC Po (Fig. 3A), whereas in ENaC + CFTR-expressing cells PKA activators routinely decreased Po (Fig. 3B). This result, coupled with the effects of CS + ATP in excised patches, strongly indicates that the CFTR-mediated regulation of whole cell amiloride-sensitive Na+ current observed previously (5) reflects modulation by CFTR of ENaC single channel gating.


Fig. 3. Effects of cAMP on open probability of ENaC studied on cell. A, cell-attached patch of ENaC only expressing cell. Pipette current was recorded at 30 mV (-Vpipette). Cell-permeant cAMP (cpt-cAMP) (500 µM) and forskolin (FSK, 10 µM) were added (as indicated by the arrow). The second and third traces were recorded 90 and 180 s later, respectively. For analysis, the Po during basal conditions (Basal, n = 8) and after stimulation (Stim, n = 8) were compared. (Histogram; p < 0.05, n = 8). B, effect of cpt-cAMP and forskolin (FSK) on ENaC activity in a cell attached patch from an ENaC plus CFTR expressing cell. Analyzed as in A. (Histogram; n = 8 in each condition). Methods: cell attached recordings were carried out under basal (Basal, prior to additions) and stimulated conditions (Stim, 3-8 min following 500 µM cpt-cAMP and 10 µM forskolin), at -Vpipette of -20 to -40 mV. A minimum of 60 s of data was analyzed from each experiment. Po was determined as above.
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The results in Figs. 2 and 3 suggest that negative regulation of ENaC by CFTR reflects an effect on ENaC activity rather than ENaC number. Additional analyses of our data support this conclusion. First, co-expression of CFTR with ENaC did not affect the number of ENaC channels observed per patch (2.17 ± 0.29 (n = 28) without CFTR and 2.29 ± 0.29 (n = 26) with CFTR). Second, the MOT of unambiguous single channel openings in excised, and cell-attached patches under optimal conditions of PKA activation were markedly decreased by the presence of CFTR (Fig. 4). Thus, CFTR negative regulation of ENAC can be explained by decreased activity of individual ENaC channels.


Fig. 4. CFTR alters cAMP regulation of ENaC kinetics (Po and MOT). Excised inside out patches or cell-attached patches that demonstrated only single ENaC during the entire experiment or patches with two channels that exhibited infrequent coincident openings were selected from the experiments presented in Figs. 2 and 3 to determine the effect of CFTR on ENaC gating in the presence of maximal PKA activity. Methods: Po was calculated as above, and lists of the durations of unambiguous openings were compiled from each experiment, with the events list feature of PClamp 6 (Axon Instruments). Very long openings precluded sufficient observations for conventional analysis of the distribution of open time durations. Accordingly, the arithmetic average of all openings greater than 40 milliseconds was calculated as an estimate of mean open time (MOT), for each experiment (minimum 60 s or 40 openings analyzed). *, ENaC + CFTR (n = 7) different from ENaC (n = 9) by unpaired t analysis (p < 0.02).
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DISCUSSION

Our data reveal a surprisingly strong positive regulation of ENaC alone by PKA. The low Po recorded in the presence of mPKI (Fig. 3) and the high Po and long MOT measured during PKA activation (Fig. 4) indicate that increasing protein phosphorylation increased the time ENaC occupied a stable open conformation. This result differs from the cAMP-dependent increase of the number of endogenous amiloride sensitive Na+ channels seen in A6 epithelial cells (9), which are reported to regulate surface expression of transport elements by membrane insertion and retrieval (12), but is similar to cAMP-dependent regulation of Po of partially purified renal (13) and lung alveolar type II cell Na+ channels (14). Studies of heterologously expressed ENaC in oocytes (15) and of reconstituted ENaC in lipid bilayers (16) detected no effect of PKA activation on single channel gating. gamma -rENaC used in our study contains two consensus PKA phosphorylation sites, but these are not highly conserved across species (6, 7). Thus, PKA regulation of ENaC gating may well involve the phosphorylation and function of an additional protein or proteins, including cytoskeletal components such as actin (17). Cell-specific expression of these proteins could explain why fibroblasts reproduce the defect in CF airways better than oocytes (15).

In intact oocytes (15), or in ENaC reconstituted in lipid bilayers after expression in oocytes (16), the presence of CFTR decreased whole cell currents or single channel open probability. Thus, CFTR appears to exert a negative modulatory regulation of ENaC in several distinct cell types, including human airway epithelia, mouse fibroblasts, and amphibian oocytes.

The present findings help explain the long standing observation that Na+ absorption across CF airway epithelia is increased and inappropriately further stimulated by cAMP (3). In CF airways, the abnormally high rate of basal Na+ absorption reflects the absence of negative regulation of ENaC by CFTR under basal phosphorylating conditions, and increased PKA activity leads only to further absorption. In contrast, CFTR function in normal airways converts the activation of PKA into a stimulus for both inhibition of ENaC-mediated Na+ absorption and stimulation of CFTR-mediated Cl- secretion. Despite previous reports of abnormal regulation of Na+ channel activity in CF (18-20), this conclusion was in doubt until now, because PKA has been reported to regulate only the number of active amiloride-sensitive Na+ channels in A6 cells (9), and because another genetic disease associated with excessive Na+ reabsorption (Liddle's syndrome) has been attributed solely to increased ENaC number (21). More recently, the mutations associated with Liddle's syndrome have been shown to act predominantly by increased ENaC Po and MOT (22). This observation, coupled with the present results, make it clear that regulation of ENaC single channel kinetics is broadly implicated in the control of epithelial sodium absorption.

A general mechanism of regulation of ion channels by ABC proteins is yet to be identified (23), but it is clear that CFTR regulates ENaC at the level of single channel gating. This observation is an important consideration for understanding the mechanism by which ABC proteins, including not only CFTR but also SUR and MDR (23), can influence other ion channels. Potentially, ABC proteins regulate the activity of other ion channels through transported substrates, as proposed for CFTR-mediated ATP release (24, 25). Alternatively, ABC proteins may regulate the activity of other ion channels by direct or indirect protein-protein interactions.


FOOTNOTES

*   The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Dagger    To whom correspondence should be addressed: Dept. of Medicine, Cystic Fibrosis/Pulmonary Research and Treatment Center, 6023 Thurston-Bowles Bldg., The University of North Carolina, Chapel Hill, NC 27599-7248. Tel.: 919-966-1077; Fax: 919-966-7524; E-mail: hoopster{at}unc.med.edu.
1   The abbreviations used are: CF, cystic fibrosis; CFTR, cystic fibrosis transmembrane conductance regulator; MOT, mean open time; ENaC, epithelial sodium channel(s); PKA, protein kinase A; CS, catalytic subunit; mPKI, myristoylated protein kinase A inhibitor; TES, 2-{[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino}ethanesulfonic acid.

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