(Received for publication, November 25, 1996, and in revised form, January 6, 1997)
From the a Department of Internal Medicine I, Division of Hematology, the c Institute of Anaesthesiology, the d Department of Nuclear Medicine, the e Institute of General and Experimental Pathology, the f Department of Vascular Biology and Thrombosis Research, and the g Department of Internal Medicine III, Division of Rheumatology, The University of Vienna, A-1090 Vienna, Austria, the h Sandoz Research Institute, A-1235 Vienna, Austria, i St. Anna-Children's Hospital, A-1090 Vienna, Austria, and the j Institute of Immunology, A-1090 Vienna, Austria
The urokinase receptor system is involved in several biological processes including extracellular proteolysis, cell invasion, and chemotaxis. Mast cells are multifunctional perivascular cells that play an important role in the regulation of microenvironmental events. We report that primary human mast cells and the human mast cell line HMC-1 express the receptor for urokinase. As assessed by Northern blotting and reverse transcription polymerase chain reaction technique, purified human lung mast cells and HMC-1 cells expressed urokinase receptor mRNA in a constitutive manner. Using a toluidine blue/immunofluorescence double staining technique and monoclonal antibodies, surface expression of urokinase receptor was demonstrable in lung, skin, uterus, heart, and tonsil mast cells, whereas the low density lipoprotein receptor-related protein was not detectable. Binding of monoclonal antibody VIM5 (recognizing the urokinase binding domain of urokinase receptor) to HMC-1 could be blocked by high molecular weight but not low molecular weight urokinase. Binding analyses performed with 123I-urokinase revealed expression of 271,000 ± 55,000 high affinity urokinase binding sites per HMC-1 cell, with a calculated dissociation constant of 1.29 ± 0.3 nM. Purified urokinase induced dose-dependent migration of primary mast cells and HMC-1 in a chemotaxis assay without inducing release of histamine. The mast cell agonist stem cell factor also induced migration of HMC-1 and caused up-regulation of expression of urokinase receptor mRNA. Together, our data show that human mast cells express functional receptors for urokinase. Expression of urokinase receptors on mast cells may have implications for mast cell-dependent microvascular processes associated with fibrinolysis, migration, or local tissue repair.
The urokinase (uPA)1-uPA receptor (uPAR) system plays a crucial role in a number of microvascular processes including local fibrinolysis, cell migration, and tissue repair (1-5). In contrast to tissue-type plasminogen activator, uPA can induce plasmin generation in the absence of fibrin. The inactive precursor form of uPA, single-chain urokinase (scuPA), can be cleaved into the active molecular form, two-chain urokinase (1-3). A number of serine proteases including plasmin, kallikrein, trypsin, and mast cell tryptase are able to convert scuPA into the two-chain form of the molecule (1-3, 6). This activation process takes place primarily at uPA binding sites expressed on the cell surface membrane of local cells in the tissues (7, 8). Binding of urokinase to uPAR is important for the generation of enzymatic activity, since receptor-bound urokinase is protected from inhibition by plasminogen activator inhibitors (PAIs) (1-3, 7, 8).
The uPAR is broadly distributed throughout the mesenchymal cell system (7-13). Surface expression of uPAR has been described for endothelial cells (10), granulocytes (11), fibroblasts (12), mesenchymal tumor cells, and macrophages (12, 13). The receptor molecule has been characterized in detail and has been cloned (14-16). The mature protein is linked to the cell interior via a glycosylphosphatidylinositol anchor (9, 14, 15). Surface uPAR can bind uncomplexed uPA for long periods of time (17-19). However, when complexed with PAI-1, uPA may be internalized together with uPAR (17-19). Apparently, expression of the PAI-1 binding low density lipoprotein receptor-related protein (LRP, CD91) plays an important role for uPA-uPAR complex internalization (17-19). Thus, receptor-ligand complexes composed of LRP, PAI-1, uPA, and uPAR can be co-internalized (17, 18). The internalization is then followed by receptor-ligand disruption in endosomes and recycling of both LRP and uPAR to the cell membrane by vesicular transport (17). The signal-transduction cascade following activation of uPAR is complex and may involve protein tyrosine kinases (20, 21). Some of the uPAR-dependent signals (e.g. chemotaxis signal) may be delivered independent of receptor-complex internalization or presence of LRP.
Mast cells (MC) are multifunctional effector cells of the immune system (22, 23). These cells produce and store vasoactive and proinflammatory mediators (24-26). In response to diverse agonists, MC can release their mediators into the extracellular space (25-27). In contrast to other hemopoietic cells, MC are extravascular cells usually located in the vicinity of small vessels and postcapillary venules in connective tissues (22, 23). A number of previous and more recent studies suggest that MC are involved in local microvascular processes such as endothelial cell activation (28), transmigration of blood cells into tissues (29, 30), and metabolism and turnover of various tissue hormones and matrix molecules (31, 32). MC also produce heparin (33) and can accumulate in areas of ongoing inflammation, tumor invasion, angiogenesis, fibrosis, or thrombosis (22, 23, 29, 30, 34-36). More recently, among other cells, MC have been implicated in the regulation of endogenous fibrinolysis (37, 38). The aims of the present study were to elucidate whether human MC express uPAR (CD87) or LRP (CD91) and whether uPAR expression is associated with a specific functional response of MC to urokinase.
Reagents and Buffers
Recombinant human (rh) stem cell factor (SCF) was purchased from Genzyme (Cambridge, MA). Collagenase type II was purchased from Sebak (Suben, Austria). Iscove's modified Dulbecco's medium, glutamine, penicillin, and streptomycin were from Life Technologies, Inc., and gentamycin, amphotericin B, and fetal calf serum (FCS) were from Sera-Lab (Crawley Down, United Kingdom). RPMI 1640 medium was from PAA Laboratories Co. (Linz, Austria). Highly purified human uPA (two-chain type; 90% high molecular weight, 10% low molecular weight type) was purchased from Laboratories Serono (Aubonne, Switzerland). Highly purified (>95%) high molecular weight urokinase (uPAh) and >95% pure low molecular weight urokinase (uPAl) were purchased from American Diagnostics (Greenwich, CT). scuPA was provided by Technoclone (Vienna, Austria). One liter of Ca2+/Mg2+-free Tyrode's buffer contained 0.2 g of KCl, 0.05 g of NaH2PO4·H2O, 0.8 g of NaCl, and 1 g of glucose.
Monoclonal Antibodies (mAb)
Antibodies against uPAR and LRP were obtained from the Fifth International Workshop and Conference on Human Leukocyte Differentiation Antigens (Boston, 1993) (39): these were the mAb L21 (subclass IgG2a; anti-uPAR), 3B10 (IgG2a; anti-uPAR), and MR19 (IgG1; anti-LRP). Anti-tryptase mAb (IgG1) was purchased from Chemicon (Temecula, CA). The mAb VIM5 (IgG1) directed against the uPA binding domain of uPAR was produced at the Institute of Immunology, University of Vienna. The anti-c-kit mAb YB5.B8 (40) (IgG1) was kindly provided by L. K. Ashman (University of Adelaide, Australia). The anti-monocyte chemotactic and activating factor mAb S14 (IgG1) served as control and was purchased from Anogen (Mississauga, Ontario, Canada).
Preparation and Culture of Mast Cells
Primary MC were prepared from surgical tissue specimens according to published techniques (41-43). Informed consent was obtained from patients in each case. Lung tissue was obtained at surgery (lobectomy or pneumectomy) from 11 patients suffering from bronchiogenic carcinoma. The tissue (5-9 g) was cut into small pieces and washed extensively in Tyrode's buffer (41). Then, tissue fragments were exposed to collagenase type II (2 mg/g of tissue) at 37 °C for 2 h. Dispersed cells were recovered by filtration through nytex cloth, incubated in FCS, and washed in RPMI 1640 medium. The primary lung cell suspensions contained 2.5-6.6% MC (by Giemsa staining). Specimens of uterus were obtained from two patients with uterine myomata. Tonsil MC were dispersed from surgical specimens removed from patients (n = 2) suffering from chronic tonsillitis. Human skin MC were dispersed from circumcised juvenile foreskin (n = 3). Lung, uterus, tonsil, and skin mast cells were isolated by use of collagenase without other enzymes. Human cardiac MC were isolated from atrial appendages of two patients suffering from cardiomyopathy (heart transplantation) as described (43). Cardiac MC were dispersed by collagenase, followed by exposure to DNase (0.5 mg/ml), hyaluronidase (0.5 mg/ml), and Pronase E (2 mg/ml). To determine the percentage and numbers of MC, cells were stained with Giemsa or toluidine blue and counted in a hematocytometer. MC were cultured at 37 °C in RPMI 1640 medium with 10% FCS, glutamine, and antibiotics.
In a series of experiments, lung MC were purified to apparent homogeneity. For this purpose, dispersed lung cells (four donors) were subjected to counter flow centrifugation (elutriation) (42). The elutriated cell fractions (n = 10) contained varying amounts of MC. In one donor, a fraction contained 91% MC and was used for Northern blotting. Fractions containing 10-55% MC were used for fluorescence staining analyses and sorting. In three donors, elutriated lung MC were further enriched by sorting with mAb YB5.B8 as described (42). After sorting, MC were >99% pure and used for rtPCR analysis or immunostaining after cytospin preparation. Enriched or highly purified MC were cultured in RPMI 1640 medium supplemented with 10% FCS and antibiotics.
The human mast cell line HMC-1 was established from a patient suffering from mast cell leukemia (44) and kindly provided by J. H. Butterfield (Mayo Clinic, Rochester, MN). HMC-1 cells were cultured in Iscove's modified Dulbecco's medium containing 10% FCS, glutamine, and antibiotics at 37 °C and 5% CO2.
Northern Blot Analysis
Primary lung MC (91% purity, 3 × 107 cells in
each sample, four samples in total) were incubated in RPMI 1640 medium
plus 10% heat-inactivated FCS in the presence (n = 2 points) or absence (n = 2) of rhSCF (100 ng/ml) at
37 °C and 5% CO2 for 2 h. HMC-1 cells (3 × 107 cells for each point) were incubated in Iscove's
modified Dulbecco's medium plus 10% FCS in the absence or presence of
rhSCF (100 ng/ml) for 2, 6, or 12 h. RNA extraction and Northern
blot analysis were performed essentially as described (45). Total
cellular RNA was extracted from cells by the guanidinium
isothiocyanate/cesium chloride method (46). Ten µg of RNA were
size-fractionated on 1.2% agarose gels and then transferred to
synthetic membranes (Hybond N, Amersham Corp.) with 20 × SSC
(1 × SSC = 150 mM NaCl, 15 mM sodium
citrate, pH 7.0) overnight. Then, RNA was cross-linked to membranes by
UV irradiation (UV Stratalinker 1800, Stratagene). Prehybridization was
performed at 65 °C for 4 h in 5 × SSC, 10 × Denhardt's solution (1 × Denhardt's solution = 0.02%
bovine serum albumin, 0.02% polyvinylpyrrolidone, 0.02% Ficoll), 10% dextran sulfate, 20 mM sodium phosphate, pH 7.0, 7% SDS,
100 µg/ml sonicated salmon sperm DNA, 100 µg/ml
poly(A)+. Hybridization was done using
32P-labeled synthetic oligonucleotide probes (Table
I) for 16 h at 65 °C in prehybridization buffer.
Probes were labeled by terminal nucleotidyl transferase and
[-32P]dATP. Blots were washed once in 5% SDS, 3 × SSC, 10 × Denhardt's solution, 20 mM sodium
phosphate, pH 7.0, for 30 min at 65 °C and once in 1 × SSC,
1% SDS for 30 min at 65 °C. Bound radioactivity was visualized by
exposure to XAR-5 film at
70 °C using intensifying screens
(Eastman Kodak Co.).
|
RNA Isolation and rtPCR
For rtPCR, total RNA was isolated from >99% pure lung MC
(2 × 104 cells each point, n = 2 donors) or purified CD19+ B-cells (47) using the
guanidinium isothiocyanate acid phenol extraction procedure
(RNAzolTM B method, Biotecx Laboratories, Houston, TX) as
reported (42). In brief, MC were centrifuged (400 × g,
10 min) and lysed in 0.8 ml of RNAzolTM B. Then, 80 µl of
chloroform were added. Samples were shaken vigorously and stored at
4 °C for 5 min. After centrifugation at 12,000 × g
(4 °C, 15 min) the upper aqueous phase was collected. Four µg of
carrier RNA (yeast tRNA, stored as 4 µg/µl of solution in
RNase-free water) were added before precipitating RNA with 0.4 ml of
isopropanol overnight at 20 °C. Precipitated RNA was centrifuged
for 15 min at 12,000 × g (4 °C). Then, the
supernatant was removed and the RNA pellet washed once with 75%
ethanol. Finally, the pellet was dissolved in 20 µl of RNase-free
water and stored in liquid nitrogen. cDNA synthesis was performed
using the first strand cDNA synthesis kit (Pharmacia Biotech Inc.)
according to the manufacturer's instructions. Briefly, total RNA was
dissolved in 20 µl of RNase-free water, heated to 65 °C for 10 min, quick-chilled on ice, and then incubated with 11 µl of bulk
first-strand reaction mix (cloned, FPLC PureR reverse
transcriptase, RNAguard, RNase/DNase-free bovine serum albumin, 1.8 mM each dATP, dCTP, dGTP, and dTTP in aqueous buffer), 1 µl of dithiothreitol solution (200 mM aqueous solution),
1 µl of pd(N)6 primer (random hexadeoxynucleotides at 0.2 µg/ml in aqueous solution) at 37 °C for 1 h. The reaction was
terminated by heating to 90 °C for 5 min. Samples were chilled on
ice immediately and stored at
20 °C for subsequent analysis.
Aliquots of the cDNA product, i.e. 3 µl for the
constitutively expressed
-actin gene as a positive internal control
of rtPCR efficiency and 15 µl for the uPAR gene (16), were used for
rtPCR. The reaction mixture contained 10 µl of 10-fold PCR buffer
(100 mM Tris-HCl, 500 mM KCl, 15 mM
MgCl2, and 0.01% (w/v) gelatin (Perkin-Elmer)), 200 µmol
of dNTP-Mix (Pharmacia), and 2.5 units of Taq DNA polymerase (Perkin-Elmer). One hundred pmol of each primer pair (5
uPAR, 23-mer,
gene position 587-609 (16): 5
-TTCCACAACAACGACACCTTCCA-3
; 3
uPAR,
23-mer, gene position 986-1008: 5
-AGGGTGATGGTGAGGCTGAGATG-3
; 5
-actin, 20-mer, gene position 969-988:
5
-AGGCCGGCTTCGCGGGCGAC-3
; 3
-actin, 21-mer, gene position
1327-1347: 5
-CTCGGGAGCCACACGCAGCTC-3
) were added to a final volume
of 100 µl. A master mix of PCR components was made up for each set of
reactions prior to addition of the cDNA templates. Primer sequences
were oriented on separate exons of the genes so that the PCR product of
the cDNA could readily be distinguished from the PCR product
amplified from any contaminating genomic DNA. Samples were then
subjected to PCR to amplify the 422-base pair DNA fragment between
nucleotides 587 and 1008 of the uPAR cDNA (16) by 35 cycles at
94 °C for 1 min and 72 °C for 1 min after initial denaturation at
95 °C for 1 min. An aliquot of each reaction mixture was subjected
to electrophoresis on a 2% agarose gel in 1 × Tris acetate-EDTA
buffer. The PCR products were visualized by ethidium bromide staining
and photographed.
Immunostaining of Surface uPAR
Combined Toluidine Blue/Immunofluorescence Staining TechniqueExpression of cell surface markers on primary MC was
analyzed by a combined toluidine blue/immunofluorescence staining
technique as described previously (48). In brief, cells were incubated with mAb for 30 min at 4 °C and washed twice in phosphate buffered saline. Cells were then conjugated with a "second step"
fluorescein-labeled goat F(ab)2 IgG + IgM anti-mouse
antibody (30 min, 4 °C). Thereafter, cells were fixed in 0.025%
glutaraldehyde solution for 1 min and incubated with toluidine blue
(0.0125%) for 8 min at room temperature. After washing, cells were
analyzed under bright field and fluorescent light with a fluorescence
microscope (Olympus, Vienna).
After exposure of HMC-1 cells to first step and second step antibody (see above), flow cytometry was performed on a FACScan (Becton Dickinson, San Jose, CA) as described (42). Standard beads, provided by the Fifth International Workshop on Human Leukocyte Typing (39), were used to calibrate the FACScan in all measurements. In each staining experiment, isotype-matched control antibodies were used. A 5% cutoff channel was set as negative/positive gate to discriminate between positive and negative cells. To confirm expression of uPA binding sites detected by the mAb, blocking experiments were performed using uPAl and uPAh. In these experiments, HMC-1 cells were preincubated with uPAl (300 units/ml), uPAh (300 units/ml), or control medium at 4 °C for 3 h. Then, cells were washed in phosphate buffered saline and incubated at 4 °C with anti-uPAR mAb VIM5 (recognizing the uPA binding domain on uPAR). After 30 min, cells were washed, exposed to the second step goat anti-mouse IgG/IgM (4 °C, 30 min), washed again, and then subjected to fluorescence-activated cell sorter analysis. Epitope blocking was quantified as the difference in mean fluorescence intensities observed between cells exposed to ligands versus cells exposed to control medium.
In Situ Staining Experiments
Lung tissue was obtained from one patient suffering from encephalomalacia (autopsy), cardiac tissue from one patient with auricular thrombosis (autopsy), and small intestine tissue from one patient with cardiac infarction (autopsy). Autopsies were part of a study approved by the local ethical committee (36). Skin tissue was obtained from juvenile foreskin (circumcision, n = 1) after informed consent was obtained. Tissue was snap-frozen in precooled isopentane and prepared for cryostat sections. Sequential double immunohistochemistry was performed using mAb to uPAR and MC tryptase (second antigen) essentially as described (36). Endogenous peroxidase was blocked by 5% H2O2/methanol. Sections were first incubated with mAb VIM5, then with biotinylated horse anti-mouse IgG, and then with streptavidin-biotin-peroxidase complexes and aminoethyl carbazole (Vector, Burlingame, CA) as chromogen, giving a reddish brown reaction product with horseradish peroxidase. Slides were then photographed. Thereafter, alkaline phosphatase-conjugated anti-tryptase mAb was applied and the reaction visualized by fast blue salt. Then, the same regions (as for VIM5) were photographed again. In control experiments, an isotype-matched control antibody was used. As a further control, staining results were verified on serial sections (heart and skin). The immunoalkaline phosphatase staining technique was applied on cytospin preparations of enriched human lung mast cells and HMC-1 cells. In these experiments the cells on cytospin slides were incubated with mAb VIM5 for 60 min, washed, and incubated with a biotinylated horse anti-mouse IgG for 30 min. Then, slides were exposed to streptavidin-alkaline phosphatase complexes. Neofuchsin was used as chromogen, giving a red reaction. Slides were counterstained in Gill's hematoxylin. Control slides were similarly treated either with the primary antibody omitted or using isotype-matched control mAb.
Labeling of uPA with 123I and Radio Receptor Analysis
Human urokinase was labeled with 123I using lactoperoxidase. For this purpose, 100 µg of uPA dissolved in 0.1 M phosphate buffer (pH 7.0) was labeled with 1 mCi of [123I]NaI (Cyclotron Research Center, Karlsruhe, Germany) by slowly mixing with 0.3 µg of H2O2 and 5 µg of lactoperoxidase (Sigma). The reaction mixture (50 µl) was injected into a reversed phase C18 high performance liquid chromatography column and eluted with a gradient of 25 to 50% MeCN in 0.1% aqueous trifluoroacetic acid. The effluent was monitored by UV (280 nm) and radioactivity detectors. The 123I-uPA peak was isolated, evaporated under vacuum, and redissolved in phosphate buffered saline. Radiochemical purity was analyzed by horizontal zone electrophoresis run on cellulose acetate stationary phase in 0.1 M barbital buffer (pH 8.6) using a field of 300 V for 10 min. Under these conditions, free [123I]iodide migrated about 45 mm as verified by an appropriate standard. 123I-uPA was obtained in 70% isolated radiochemical yield at a specific activity of about 4 mCi/mg. Radiochemical purity was more than 97% and remained stable for at least 20 h.
The receptor assay was performed using HMC-1 cells essentially as described (49). In a first set of experiments, specific binding of 123I-uPA to intact HMC-1 cells was analyzed as a function of time. In saturation experiments, HMC-1 cells were incubated with increasing concentrations (0.01-8.0 nM) of 123I-uPA in the presence or absence of unlabeled ligand (500 nM). Experiments were done in duplicate and performed six times. In saturation experiments, cells were incubated with 15 nM 123I-uPA at 4 °C for 45 min in the presence or absence of increasing concentrations (0.01-500 nM) of unlabeled ligand. The binding data were analyzed according to Scatchard.
Chemotaxis Assay
Mast cell migration was quantified using a 24-well double chamber chemotaxis assay as described recently (50). Briefly, lung MC (n = 5), skin MC (n = 3), or HMC-1 cells were resuspended in RPMI 1640 medium and adjusted to a final cell concentration of 3 × 106 cells/ml. In initial experiments, the agonists, i.e. various concentrations of rhSCF (1, 10, and 100 ng/ml), uPA (0.2-400 nM; 1 nM corresponds to 5 units/ml), or control medium (RPMI 1640) were placed into the lower chamber of the wells. Then, microporous filter membranes (0.6 cm2; pore size, 3.0 µm; Cyclopore, Aalst, Belgium) were inserted. Thereafter, MC were placed in the upper chambers and incubated at 37 °C in 5% CO2 for 3 h. The membranes were then detached and removed together with nontransmigrated cells. The migrated HMC-1 cells in the lower chamber were incubated with the fluorescence dye Calcein AM (5 mM; Molecular Probes, Eugene, OR) for 30 min at room temperature. Then, labeled HMC-1 cells were measured in a multiplate reader (Biosearch, Hamburg, Germany), and the number of migrated cells was calculated by the calculation program delivered by the manufacturer (Biosearch). In "blocking experiments," MC or HMC-1 were preincubated with mAb VIM5 (recognizing the uPA binding domain of uPAR) or an isotype-matched control mAb before starting chemotaxis experiments. In the case of primary MC, the cellular histamine values in transmigrated cells were measured as an objective parameter of MC migration (uPA did not induce histamine secretion). To delineate the enzymatic effect of uPA from its migration-inducing effect, chemotaxis experiments were performed on HMC-1 cells using natural, active uPA, enzymatically inactive scuPA, and diisopropyl fluorophosphate (Hoechst, Vienna, Austria) -treated uPA (DFP-uPA). DFP-uPA showed less than 5% specific enzymatic activity compared with untreated uPA. To differentiate between directed migration (chemotaxis) and nondirected migration (chemokinesis) of cells, checkerboard analyses were performed. In these experiments, the agonist uPA was placed into either the lower or upper wells, or both, of the chamber assay before cells were added.
Histamine-release Experiments
Histamine-release experiments were carried out on lung MC
(n = 7). Experimental conditions were essentially as
described earlier (51). MC were exposed to various concentrations of
uPA (uPAl or uPAh) for 30-90 min at 37 °C in 5% CO2.
For IgE-dependent release, MC were preincubated with
myeloma IgE (myeloma cell line U266) for 3 h at 4 °C, washed,
and resuspended in histamine-release buffer (Immunotech, Marseille,
France). In selected experiments (n = 2), lung MC were
preincubated with uPAh (150 units/ml, 30 nM), uPAl (150 units/ml), rhSCF (1 ng/ml), or control medium for 15 min prior to
anti-IgE activation. After preincubation, MC were exposed to various
concentrations of the anti-IgE mAb E-124-2-8 (0.1-10 µg/ml) for 30 min in 96-well microtiter plates (Costar, Cambridge, MA) at 37 °C
for 30 min. Thereafter, cells were centrifuged at 4 °C and the
cell-free supernatants recovered and analyzed for the amount of
(released) histamine. Total histamine (extracellular plus
intracellular) was quantified in whole cell suspensions. Histamine
release was calculated and expressed as percentage of total histamine.
Histamine was measured in supernatants and cell lysates by a
radioimmunoassay (Immunotech) as described (48, 51). This assay showed
a detection limit of 0.2 nM and no cross-reactivity with
heparin, tryptase, rhSCF, tumor necrosis factor , or other cytokines.
Statistical Analysis
Standard tests including Student's paired t test were applied to evaluate the significance of differences in the results. Results were considered significantly different when the p value was <0.05.
Northern blot
analysis and rtPCR revealed expression of uPAR mRNA in primary
human lung MC and HMC-1 cells. In Northern blot experiments, primary
lung MC (91% pure) and HMC-1 cells were found to express uPAR mRNA
in a constitutive manner. Resting HMC-1 cells expressed significant
amounts of uPAR mRNA. In contrast, the level of constitutively
expressed uPAR mRNA in unstimulated primary MC was rather low (Fig.
1A). To exclude a signal delivered by contaminating cells, rtPCR analysis was applied using highly enriched (>99% pure) lung MC. These highly purified unstimulated MC expressed uPAR mRNA as determined by rtPCR (Fig. 1B). In Northern
blot experiments, a small increase in expression of uPAR mRNA in
lung MC was found after incubation of cells with the MC agonist rhSCF
(100 ng/ml, 2 h) (Fig. 1A). Similar results were
obtained using the HMC-1 cell line; again, exposure of HMC-1 cells to
rhSCF (100 ng/ml, 2-12 h) resulted in an increased expression of uPAR
mRNA (Table II). Urokinase or LRP mRNA were not
expressed in unstimulated MC or in SCF-stimulated MC (Table II). In
addition to uPAR and LRP, several control genes were examined by
Northern blotting. "Positive control genes"
(glyceraldehyde-3-phosphate dehydrogenase, c-kit) were found
to be transcribed in primary MC and HMC-1, whereas "negative control
genes" (c-fms, CD25, SCF) were not (Table II). rtPCR was
controlled by using primers specific for T-cell receptor chain and
bcl-2, giving negative results for pure MC (see Ref. 42), thereby
excluding the presence of significant levels of RNA from contaminating
cells.
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In a first set of experiments, the reactivity of primary human MC obtained from lung, skin, uterus, heart, and tonsils with mAb clustered as CD87 (uPAR) was assessed by staining of cells with both toluidine blue and indirect immunofluorescence. MC from all organs tested were recognized by the anti-uPAR mAb L21 and 3B10 (Table III). More than 80% of the MC were stained by these mAb, irrespective of the origin of MC. In contrast, the tissue MC showed little or no surface reactivity (<10% of MC) with mAb VIM5 directed against the uPA binding domain of uPAR (Table III). Exposure of primary MC to pH 3.8 (30 min) resulted in an increased reactivity of MC with VIM5. However, the cells also showed an increased uptake of trypan blue compared with untreated cells.
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Binding of anti-uPAR mAb could also be demonstrated for the human MC
line HMC-1 (Table III). All three anti-uPAR mAb including VIM5 bound to
HMC-1 cells at pH 7.4. Binding of VIM5 antibody was inhibitable by
preincubation of HMC-1 cells with uPAh but not by uPAl (mean
fluorescence intensity: control (2.9) versus VIM5 (37.4)
versus VIM5 + uPAh (10.3) versus VIM5 + uPAl
(31.8)) (Fig. 2). Incubation of MC or HMC-1 with rhSCF
(100 ng/ml, 37 °C, 2-12 h) resulted in an increased expression of
uPAR in fluorescence-activated cell sorter analyses (VIM5) compared
with control (more than 2-fold increase in mean fluorescence intensity)
(not shown). LRP was not detectable on either the surface of primary MC
or on HMC-1 (Table III).
In Situ Detection of uPAR in Human Mast Cells
To confirm uPAR
expression in MC, in situ staining experiments on tissue
sections were performed. The VIM5 mAb was used in these studies, since
the mAb was found to recognize the uPAR in the cytoplasm of HMC-1
cells. As assessed by double immunoperoxidase staining using VIM5 and
anti-tryptase mAb, MC in all organs tested (lung, skin,
gastrointestinal tract, and heart) were found to express uPAR. VIM5
labeling was found in cytoplasmic compartments of MC and showed a
granular pattern. Almost all MC were labeled by the anti-uPAR mAb VIM5.
Other cells in the tissues, including vascular cells, were also found
to react with VIM5. Fig. 3 shows in situ
double immunoperoxidase staining of one skin MC for uPAR (Fig.
3A) and tryptase (Fig. 3B). The uPAR could also
be detected in purified lung MC (Fig. 3C) and HMC-1 (Fig.
3E) by immunostaining using cytospin slides and mAb
VIM5.
Characterization of 123I-uPA Binding Sites on HMC-1 Cells
In initial experiments, the time course of association and
dissociation of 123I-uPA binding to HMC-1 cells was
analyzed. Association of binding showed a rapid increase and reached an
apparent equilibrium within 20 min of incubation (Fig.
4). The calculated association rate constant
k1 (ln 2/ × Lo (ligand concentration
at time point "0");
= 234 s) was 5.92 × 10
5 M
1 s
1.
Binding of 123I-uPA to HMC-1 membranes rapidly declined
following addition of an excess (500 nM) of unlabeled uPA
(Fig. 4). The dissociation rate constant
k
1 (ln 2/
;
= 378 s) was
1.8 × 10
3 s
1. The
Kd value (1.3 × nM) of our
saturation experiments thus fits quite well with the time rate
constants (Kd = k
1/k1 = 3 × nM), both being in the lower nanomolar range.
Receptor binding experiments using 123I-uPA and HMC-1 cells
revealed specific binding at 4 °C. To assess whether the binding behavior of unlabeled uPA differs from that of 123I-labeled
uPA, binding experiments with a constant uPA concentration (5 nM) but different proportions of unlabeled to labeled uPA
were performed. In these experiments, no significant difference in the
binding behaviors between unlabeled and labeled ligand was found (not
shown). Binding of 123I-uPA to HMC-1 cells was displaced by
addition of unlabeled uPA, reaching an IC50 value of
5.1 ± 0.9 nM (Fig. 5). Scatchard plot analysis of binding of 123I-uPA to HMC-1 cells revealed a
single class of 271,000 ± 55,000 high affinity uPA binding sites
with a calculated Kd of 1.29 ± 0.3 nM (Fig. 6, A and
B).
uPAR-mediated Migration of Human Mast Cells
To demonstrate a
specific function for the uPAR on MC, a chemotaxis assay was applied.
uPA induced a chemotactic response both in primary lung MC (control,
100 ± 18.4%; 20 nM uPA, 640.7 ± 42.3%) and
skin MC (control, 100 ± 5.9%; 20 nM uPA, 1630 ± 97%) as well as in HMC-1 cells (control, 100 ± 18%; 300 nM uPA, 179 ± 44%) (Figs. 7, 8, 9).
Figs. 7 and 9 show the migration-inducing effect of uPA on HMC-1 cells,
and Fig. 8 shows the effect of uPA on lung (Fig.
8A) and skin (Fig. 8B) MC. The migration-inducing effect of uPA on MC was dose-dependent with optimal
concentrations ranging between 0.2 and 20 nM (1 and 100 units/ml) for primary MC (p < 0.05) and between 150 and 300 nM (750 and 1500 units/ml) (p < 0.05) for HMC-1 cells (Figs. 7 and 9). SCF also induced MC chemotaxis
in these experiments (optimal concentration, 10-100 ng/ml) and
cooperated with uPA in the induction of chemotaxis in HMC-1 (Fig. 7).
To provide evidence that the migration-inducing effect of uPA was
mediated via uPAR, antibodies against the uPA binding domain of the
uPAR (VIM5) were used in blocking experiments. In these experiments,
lung MC or HMC-1 were preincubated with mAb VIM5 (10 µg/ml) or an
isotype-matched control antibody for 30 min at 4 °C. Then, cells
were washed and added to the chamber system. Preincubation with mAb
VIM5 resulted in an almost complete inhibition of uPA-induced migration
(p < 0.01) of HMC-1 cells (Fig. 7) and lung MC (Fig.
8A), whereas a control antibody did not block
uPA-dependent migration (Fig. 7).
To determine whether the migration-inducing effect of uPA on MC is dependent on the enzymatic activity of the ligand (uPA), chemotaxis experiments were performed with the enzymatically inactive single-chain precursor of uPA (scuPA) and with DFP-uPA that exhibited less than 5% of the specific enzymatic activity when compared with untreated uPA. In these experiments, both scuPA and DFP-uPA induced chemotaxis of HMC-1 cells similar to natural purified active uPA (Fig. 9).
To discriminate between chemokinesis (undirected migration) and chemotaxis (directed migration) of MC against uPA, checkerboard analyses were performed using HMC-1 cells. These experiments revealed a (directed) chemotactic response of human MC against urokinase (Table IV).
|
According to previous
observations, anti-IgE (after preincubation of cells with IgE) induced
histamine release from lung MC (Fig. 10), and
preincubation of MC with rhSCF resulted in an increased response to
anti-IgE (Fig. 10B). By contrast, between 1.5 and 150 units/ml (lower nanomolar range, i.e. concentrations
inducing chemotaxis in lung MC) uPA (uPAl and uPAh) failed to induce
histamine secretion in MC (Fig. 10A). Furthermore, in
contrast to rhSCF, uPAl and uPAh did not promote (or inhibit)
IgE-dependent release of histamine from MC (Fig.
10B). The anti-uPAR mAb VIM5 (10 µg/ml), L21 (10 µg/ml),
and 3B10 (10 µg/ml) were also tested (cross-linking of uPAR) but did
not induce histamine release (Fig. 10A).
A number of previous and more recent observations suggest that MC are involved in several microvascular processes such as activation of endothelial cells, vasodilation, capillary leak formation, or transmigration of blood-derived cells into tissues (22, 28-30). Moreover, MC and their products have been implicated in the process of extracellular proteolysis and fibrinolysis (24, 31, 37, 38). The receptor for urokinase is an important cellular antigen that mediates fibrinolysis, cell migration, and tissue repair in general (7-11, 52, 53). The results of this study demonstrate expression of uPAR on primary tissue MC and the human mast cell line HMC-1. Expression of the receptor for uPA was demonstrable by indirect immunofluorescence staining experiments, by in situ staining, by Northern blot analysis, and by rtPCR. The functional significance of this MC receptor was also demonstrable; in particular, this receptor apparently mediates MC chemotaxis.
The uPAR has recently been clustered as CD87 (39). In this study, three different mAb clustered as CD87 were found to bind to MC. One of these antibodies, VIM5, is directed against the uPA binding domain of the uPAR (39). Correspondingly, preincubation of HMC-1 cells with high molecular weight (but not low molecular weight) uPA resulted in a significant loss of reactivity with mAb VIM5, whereas the binding of other mAb against uPAR was not altered. The VIM5 domain of the uPAR was detectable on the surface of intact HMC-1 cells as well as by in situ (cytoplasmic) staining of primary tissue MC or HMC-1 cells. However, almost no surface reactivity of primary MC with mAb VIM5 was found, although the other anti-uPAR mAb showed significant reactivity. The most likely explanation for this phenomenon is receptor coverage by endogenous ligand (uPA) (4) or by other surface molecules. Alternatively, the VIM5 epitope is constantly shed from the MC surface. The fact that mAb VIM5 bound more effectively to MC at pH 3.8 than at pH 7.4 would be in line with the "coverage hypothesis." However, since MC at pH 3.8 show increased trypan blue uptake (due to disrupted membranes), the reactivity of VIM5 with MC at low pH (3.8) may also be due to binding to intracellular uPAR. The possibility that MC do not synthesize the VIM5 epitope seems rather unlikely, since the in situ staining experiments showed a clear reactivity of mAb VIM5 with the cytoplasm of MC and since uPA-induced chemotaxis of MC was inhibitable by the mAb VIM5.
So far, little is known about the regulation of expression of uPAR in MC. In this study, human MC expressed uPAR mRNA and surface uPAR in a constitutive manner, although the amount of expressed uPAR mRNA in unstimulated primary MC was rather low. However, an increase in expression of uPAR mRNA was found after stimulation of primary MC (and HMC-1 cells) with the MC agonist SCF. This cytokine, SCF, is a well recognized stimulator of MC differentiation, survival, and activation (54-57). The observation that SCF augments expression of uPAR in MC further supports the concept that this cytokine is a major regulator of MC.
The binding behavior of uPA to MC membranes was analyzed by a receptor assay using radiolabeled uPA and intact HMC-1 cells. In these experiments, HMC-1 cells expressed approximately 200,000-300,000 high affinity 123I-uPA binding sites with a calculated Kd of 1.29 ± 0.3 nM. A similar range of uPAR has recently been described for blood monocytes, vascular endothelial cells, and tumor cells (4, 7, 12, 13, 15, 58). The number and binding constants of uPAR expressed on primary tissue MC could not be determined in this study because of the difficulty of purifying enough cells. However, when comparing fluorescence intensities for anti-uPAR mAb (L21 and 3B10), the numbers of uPAR expressed on primary MC might be in a lower range compared with HMC-1.
The fate of receptor-bound uPA depends on the cell type, the mobility of the receptor, and the presence of additional molecules. Thus, uncomplexed (free of PAIs) uPA may be expressed in association with uPAR on the cell membrane for prolonged periods of time without significant receptor turnover, internalization, or shedding (7, 17-19). However, in the presence of PAI-1 and LRP, uPAR may be internalized (17, 18). In this study, human MC were found to express uPAR but not uPA or LRP. Thus, endogenous receptor-bound uPA on MC may derive from neighboring cells (but not mast cells) and usually not be internalized by a LRP/PAI-1-dependent mechanism. These observations would favor the hypothesis that active uPA is expressed on MC in tissues for prolonged time periods. In this respect it is also noteworthy that MC are a unique source of tryptase (59), an enzyme that effectively activates uPA (6). Additionally, since MC are a source of uncomplexed tissue-type plasminogen activator (37, 38) but not PAIs, the currently favored concept is that the mast cell is a primary site of tissue fibrinolysis.
Recent data suggest that uPAR is not only a cellular substrate of endogenous fibrinolysis but also a "chemotaxis receptor" (4, 9, 39, 52, 53). We therefore asked whether uPA could be a MC chemoattractant. The results of this study show that uPA induces a significant chemotactic response in both primary human tissue MC and the human mast cell line HMC-1. The reason for the differences between HMC-1 cells and primary MC regarding their responsiveness to uPA (different effective concentrations) are at present unknown. The range of the dissociation constant of the uPAR on HMC-1 (lower nanomolar range) fits quite well with the concentrations of uPA that induced chemotaxis in primary MC but fits less well with concentrations of uPA that could induce migration of HMC-1. One possibility could be that HMC-1 cells lack a potent signal transducer (such as a co-expressed surface signal-transducer molecule) required for induction of chemotaxis. The fact that preincubation of MC with mAb VIM5 (against the uPA binding domain of the uPAR) was followed by a significant blockage of uPA-induced chemotaxis strongly suggests that the effect of uPA was mediated via the uPAR in both types of cells.
We also asked whether uPA can influence mast cell functions other than chemotaxis. However, in the present study, uPA did not induce or promote release of histamine from human MC. This is in contrast to SCF, another product of activated endothelial cells. Thus, SCF, unlike uPA, was able to augment both chemotaxis and histamine release in MC, confirming earlier observations (57, 60). An interesting aspect is that SCF promotes expression of uPAR on MC. Thus, SCF and uPA may cooperate through multiple mechanisms in the induction of MC chemotaxis and MC accumulation in tissues.
Together, our data show that human mast cells express functional uPA receptors. These receptors may be involved in the accumulation of MC and in mast cell-dependent processes associated with fibrinolysis.
We thank Doris Gludovacz, Petra Buchinger, Dieter Printz, and Hans Semper for skillful technical assistance.