(Received for publication, November 14, 1996, and in revised form, April 11, 1997)
From the Laboratory of Biochemistry and ¶ Laboratory of
Pathology, NCI, National Institutes of Health, Bethesda, Maryland 20892 and the Department of Biological Sciences, Purdue
University, West Lafayette, Indiana 47907
We describe a dominant negative (DN) to activation protein-1 (AP1) that inhibits DNA binding in an equimolar competition. AP1 is a heterodimer of the oncogenes Fos and Jun, members of the bZIP family of transcription factors. The DN, termed A-Fos, consists of a newly designed acidic amphipathic protein sequence appended onto the N-terminus of the Fos leucine zipper, replacing the normal basic region critical for DNA binding. The acidic extension and the Jun basic region form a heterodimeric coiled coil structure that stabilizes the complex over 3000-fold and prevents the basic region of Jun from binding to DNA. Gel shift assays indicate that A-Fos can inactivate the DNA binding of a Fos:Jun heterodimer in an equimolar competition. Transient transfection assays indicate that A-Fos inhibits Jun-dependent transactivation. Both the acidic extension and the Fos leucine zipper are critical for this inhibition. Expression of A-Fos in mouse fibroblasts inhibits focus formation more than colony formation, reflecting the ability of A-Fos to interfere with the AP1 biological functions in mammalian cells. This reagent is more potent than a deletion of either the Fos or Jun transactivation domain, which has been used previously as a dominant negative to AP1 activity.
The activation protein-1 (AP1)1 transcription factors
are immediate early response genes involved in a diverse set of
transcriptional regulatory processes (1). The AP1
complex consists of a heterodimer of a Fos family member and a Jun
family member. This complex binds the consensus DNA sequence (TGAGTCA)
(termed AP1) sites found in a variety of promoters (2, 3). The Fos
family contains four proteins (c-Fos, Fos-B, Fra-1, and Fra-2) (4-6),
while the Jun family is composed of three (c-Jun, Jun-B, and Jun-D)
(7-10). Fos and Jun are members of the bZIP family of
sequence-specific dimeric DNA-binding proteins (11). The C-terminal
half of the bZIP domain is amphipathic, containing a heptad repeat of
leucines that is critical for the dimerization of bZIP proteins (12, 13). The N-terminal half of the long bipartite -helix is the basic
region that is critical for sequence-specific DNA binding (14-16).
To dissect the function of the AP1 complex in cellular processes, investigators have used dominant negatives (DNs) to AP1 consisting of a deletion of the transactivation domain of either a Jun family member (17-19) or a Fos family member (20, 21). These truncated Fos or Jun proteins dimerize with endogenous transcription factors, which results in the loss of AP1 activity (21, 22). A conceptual disadvantage with this strategy is that the heterodimer between the endogenous transcription factor and the dominant negative still binds DNA, which makes it difficult to document a change in DNA occupancy that correlates with the expression of the dominant negative. A dominant negative that is deleted for the DNA binding domain would overcome this type of problem, but such potential dominant negatives do not work well because of the stabilization that occurs when bZIP proteins bind DNA (16, 23). We are interested in developing DNs (24) that stoichiometrically inhibit the sequence-specific DNA binding of the AP1 complex. These reagents should inhibit AP1 DNA binding in vivo, thus allowing us to monitor occupancy of AP1 cis elements in vivo (25).
We previously demonstrated that the DNA binding of the bZIP protein C/EBP could be inhibited stoichiometrically by appending an amphipathic acidic extension to the N-terminus of the C/EBP leucine zipper (26). We explored the generality of this strategy by appending the same acidic extension onto the Fos leucine zipper. This construct (4H-Fos) was not able to inhibit AP1 DNA binding in an equimolar competition. This paper describes a newly designed amphipathic acidic extension (termed A- or N4H-), which, when appended onto the N-terminus of the Fos leucine zipper, is able to inhibit the DNA binding of AP1 in an equimolar competition. When expressed in mammalian cells, A-Fos inhibits Jun-dependent transactivation and dramatically reduces Ha-ras-mediated cellular transformation in a leucine zipper-dependent fashion.
The Fos, Jun, VBP, and CREB bZIP domains were
constructed by polymerase chain reaction and cloned into the
prokaryotic expression vector pT5 as NdeI-HindIII
fragments (26). All of the proteins have a 13-amino acid N-terminal
10 leader ASMTGGQQMGRDP.
The human Fos bZIP domain spans from Lys128 to Asp208; chicken Jun bZIP domain spans from Ser222 to Phe310, the natural COOH terminus; chicken VBP bZIP domain spans from Lys232 to Leu311, the COOH terminus; mouse CREB bZIP domain spans from Leu274 to Asp341, the COOH terminus. The C/EBP bZIP domain has been described previously (26).
The protein sequences of the acidic extensions of the dominant
negatives are as follows. The last L in the following sequences is the first d position (see Fig. 1) of the Fos leucine zipper (14), and for cloning convenience, the Q following the
first L of the Fos leucine zipper has been changed to an E to produce a
XhoI site: 4H-Fos,
DLEQRAEELARENEELEKEAEELEQENAELE; A-Fos,
DLEQRAEELARENEELEKEAEELEQELAELE. L is
the amino acid changed in the new acidic extension. The following VBP,
CREB, and C/EBP leucine zippers have been cloned downstream the acidic
extension as XhoI-HindIII fragments. The
construct A-VBP contains Asp in the a position of the acidic
extension. The acidic extensions fused to the leucine zippers have been
cloned as NdeI-HindIII fragments by polymerase
chain reaction into the pT5 vector. The construct 0H-Fos contains the
Fos leucine zipper that spans from Leu165 to
Asn208 and also has the mutation Q to E mentioned
above.
Protein Purification
Proteins were expressed in Escherichia coli, and those capable of binding DNA were purified over a heparin column as described previously (26), and subsequently purified over a Rainin HPLC system using a C18 column chromatographed from 0 to 100% acetonitrile in 0.1% trifluoroacetic acid. The proteins lacking DNA binding domains were purified over a hydroxylapatite column, eluted with 200 mM phosphate, and subsequently purified on a Rainin HPLC system as just described. The protein purification protocol was modified to purify the Jun bZIP protein. The initial pellet of the Jun sample was resuspended in 1 M KCl and centrifuged at 25,000 rpm. The pellet was gently brought to 5 M urea, sonicated, heated at 65 °C for 15 min, and centrifuged, and the supernatant was isolated. The proteins were dialyzed to 50 mM KCl, 20 mM Tris, pH 8, 1 mM EDTA and loaded onto a heparin column as described before (26). The molar concentrations were calculated as described previously (26). The AP1 complex was purified from T cells as described previously (45).
Circular DichroismTm values were
calculated as described before (46), converted to
Kd (37) and G(37) using a
Cp of
1.4 kcal/mol/ °C calculated from a
Tm versus
H plot for all of
the proteins used in this study. All thermal melts were reversible. The
spectra were recorded in a 0.5-cm cuvette.
Proteins (2 µl of 5 × 106 M dimer) were heated for 10 min at
65 °C in the presence of 1 mM dithiothreitol and added
to 20 µl of the gel shift reaction buffer (25 mM Tris (pH
8.0), 50 mM KCl, 0.5 mM EDTA, 2.5 mM dithiothreitol, 1 mg/ml bovine serum albumin, 10%
glycerol), incubated for 10 min at 25 °C, and then mixed with 8 pg
of the probe (32P-labeled double-stranded oligonucleotide
containing the AP1 site). The binding complexes were resolved on an 8%
polyacrylamide gel in 0.5% TBE buffer at room temperature. The
sequence of the AP1 probe is GTCAGTCAGTGACTCAATCGGTCA. The
sequence of the CREB probe is GTCAGTCAGTGACGTCAATCGGTCA. The
sequence of the VBP probe is GTCAGTCAGATTACGTAATATCGGTCA.
The DNA binding sites are in boldface type. The conditions used for the
DNA binding assay of the AP1 complex purified from T cells have been
described previously (34). The supershift analysis was performed using 3 µl of anti-JunD antibody (34) or anti-NF
B antibody (Santa Cruz
Biotechnology, Inc.) added to the binding reactions for 30 min before
adding the AP1 probe.
Transient transfections by calcium
phosphate were carried out in HepG2 cells as described previously (47).
Cells were transfected with 20 µg of DNA consisting of 10 µg of
reporter, 3 µg of Jun transactivator, and 3 µg of dominant negative
and salmon sperm DNA. After 2 days, cells were harvested and assayed
for chloramphenicol acetyltransferase (CAT) activity. CAT activities
were normalized to protein concentration and represented as -fold
activation over the reporter plasmid alone. The conditions for
transfecting the C/EBP transactivator and the C/EBP reporter gene
were identical except that 0.3 µg of transactivator and 0.3 µg of
dominant negative were transfected (26). Jurkat cells (107
cells) were suspended in 225 µl of RPMI complete medium containing 20% fetal calf serum at 4 °C. The addition of 8 µg of the
reporter construct p10, 1 µg of Rous sarcoma virus
-galactosidase,
and different concentrations of the dominant negative 4H-Fos was
followed by electroporation (200 V, 1180 microfarads) (48). The cells were cultured for 2 h in complete medium and then stimulated with phytohemagglutinin (2 µg/ml) and phorbol 12-myristate 13-acetate (50 ng/ml), and the cells were harvested after 18 h. CAT assays were
performed in triplicate.
The HepG2 cells were transfected with 10 µg of expression plasmid and 10 µg of carrier DNA. The cells were harvested in 250 mM Tris, pH 8, containing protease inhibitors (1 mM phenylmethylsulfonyl fluoride, 1 mM aprotinin A, 1 mM leupeptin, 1 mM pepstatin, 1 mM dithiothreitol, 0.5 mM EDTA, and 0.5 mM EGTA), the extract was freeze-thawed three times, and 30 µg of extract were run on a 16% acrylamide gel. After transfer, the membrane was probed with the FLAG M2 antibody (Eastman Kodak Co.) at a concentration of 0.5 µg/ml. The ECL kit from Amersham Corp. was used for SDS polyprotein detection.
Indirect ImmunofluorescenceHepG2 cells were cultured in 1-ml slide flasks (Nunc). Cells were fixed in 4% formaldehyde in PBS for 20 min at room temperature followed by methanol for 10 min. After blocking with 3% bovine serum albumin in PBS and 0.1% Tween 20 for 30 min at room temperature, slides were incubated with a 1:200 FLAG M2 antibody and 3% bovine serum albumin in PBS for 2 h, followed by incubation for 1 h with fluorescein isothiocyanate-conjugated rat anti-mouse antibody used at a 1:200 dilution in PBS containing 3% bovine serum albumin. After each incubation with antibodies, cells were extensively washed with PBS, 0.05% Tween 20 twice for 10 min each at room temperature.
Eukaryotic PlasmidsThe eukaryotic expression plasmid containing chicken Jun is driven by the CMV promoter and has been described elsewhere (21). The CAT reporter plasmid containing a single AP1 binding site has been constructed by inserting the AP1 consensus site AGCTTGGATCCAGATCGAGCCCCAATGACTCATCATAGA in front of a minimal promoter p-35 Alb CAT described previously (47) and has been used for transfection in HepG2 cells. The CAT reporter plasmid p10 used for transfection experiments in Jurkat cells consists of a chimeric c-fos promoter gene fusion carrying the Gibbon ape leukemia virus-TPA-responsive element enhancer and has been described previously (49). Dominant negative coding sequences (0H-Fos, 4H-Fos, A-Fos, A-VBP, 4H-CREB, A-CREB) were cloned as NdeI-HindIII fragments into pRc/CMV vector (Invitrogen) modified to contain a N-terminal hemagglutinin epitope (MYPYDVPDYA) pRc/CMV566 or an N-terminal FLAG epitope (MDYKDDDK) and a new polylinker. The NdeI-HindIII fragments were obtained from the prokaryotic expression vector pT5 in which the dominant negatives had been cloned previously (see "Proteins").
Stable TransfectionsStable transfection of the murine
fibroblast cell line C3H10T1/2 (ATCC number CCL226) was
performed as described previously (29) using the calcium phosphate DNA
precipitation method. Individual precipitates containing 200 ng of pT24
Ha-ras (30) and 600 ng of each pRc/CMV566 construct were
added to two 100-mm tissue culture dishes, each seeded 24 h prior
to transfection with 5 × 105 cells and treated 2 h before the addition of precipitates with medium containing 10 µg/ml
chloroquine (Sigma). To assess focus formation, 24 h after
transfection, the cultures were split 1:3 and maintained in basal
modified Eagle's medium supplemented with 5% fetal calf serum. To
assess colony formation, of the cell suspension obtained
from both plates was further divided 1:6 and maintained in basal
modified Eagle's medium supplemented with 10% fetal calf serum and
400 µg/ml Geneticin (G-418 sulfate, Life Technologies, Inc.). After
14 days, the plates were stained with Giemsa (EM Diagnostic Systems,
Darmstadt, Germany), and the efficiency of both focus formation and
colony formation was determined by visual inspection. The efficiency of
focus formation was calculated for each experimental group based on the
number of foci obtained from a parallel group transfected with either
Ha-ras or with Ha-ras plus pRc/CMV566 DNA, which
is set at 1.00. The efficiency of colony formation was calculated for
each group based on the number of colonies obtained from a group
transfected with Ha-ras plus pRc/CMV566, which is set at
1.00. The values reported (in most instances) have been calculated from
the numbers obtained from multiple, independent experiments. The ratio
of colony forming efficiency (CE) to focus formation efficiency (FFE)
assesses the relative contribution of a decrease in CE to the observed
level of FFE. CE/FFE values greater than 1.0 are considered
significant.
Previously, we showed that three bZIP basic regions from C/EBP, VBP, and GBF1, when appended onto the N-terminus of the C/EBP leucine zipper, were able to form a heterodimeric coiled coil structure with a designed acidic amphipathic protein sequence. The acidic amphipathic extension had been appended onto the N-terminus of a leucine zipper designed to preferentially interact with the C/EBP leucine zipper (Fig. 1) (26, 27). This acid amphipathic extension (4H-) of the leucine zipper created a potent DN that heterodimerized with the bZIP protein C/EBP and prevented DNA binding. We explored the generality of this method by appending the acidic amphipathic extension onto the Fos leucine zipper in an attempt to inhibit Fos:Jun DNA binding. The rationale was that the acidic extension would electrostatically mimic DNA and provide the Jun basic region with an alternate interaction surface. The Jun basic region, instead of binding in the major groove of DNA, would form a heterodimeric coiled coil structure with the acidic amphipathic protein sequence.
The above strategy was unsuccessful because the Jun basic region is different from the three basic regions examined previously (Fig. 1). There is a hydrophobic amino acid (isoleucine) in the a position immediately N-terminal of the first d position of the Jun leucine zipper, while the three previous basic regions (C/EBP, VBP, and GBF1) contained a polar amino acid (asparagine, glutamate, or cysteine) in this position. Previously, we placed an asparagine in the corresponding position of the acidic extension (4H-) to create a polar interaction in the hydrophobic interface as is seen in the leucine zipper, which also contains an N in the a position (28). In the new acidic extension (N4H-) (Fig. 1), we have replaced this polar asparagine within 4H- with a hydrophobic leucine, reasoning that the hydrophobic isoleucine of the Jun basic region would interact more favorably with a leucine than an asparagine. For simplicity, we refer to N4H-Fos as A-Fos, where A refers to acidic extension.
The New Acidic Extension of the Fos Zipper Stabilizes the Interaction with the Jun bZIP Domain 3000-foldThe thermal
stability of mixtures of the Jun and Fos bZIP domains and the Jun bZIP
domain with different potential dominant negatives was monitored using
CD spectroscopy (Fig. 2, Table I). The
dissociation constants were calculated at 37 °C because they provide
information about dimerization in vivo. The Fos bZIP domain is so unstable that we were unable to determine reliably a dissociation constant. The Jun bZIP domain, however, does produce an interpretable thermal melt with a Kd (37) = 105 M (Table I). The mixture of Fos and Jun
forms a heterodimer. This is demonstrated by the greater stability of
the mixture (Kd (37) = 9 × 10
8 M), which is greater than the sum of the
individual Fos and Jun thermal melts (solid line) (Fig.
2A). The deduced Kd (25) for
a Fos and Jun mixture is 1.3 × 10
8 M,
which is similar to the value of
Kd (25) = 2.3 × 10
8
M reported earlier using fluorescence energy transfer assay
(23).
Stability of the acidic extension appended to Fos zipper with Jun. A, CD thermal melting curves at 222 nm of 1) Jun, 2) Fos, 3) Jun + Fos, 4) Jun + 0H-Fos (the Fos zipper without the basic region), 5) Jun + 4H-Fos, and 6) Jun + A-Fos. The melts of Fos, Jun, and the mixture (Jun + Fos) are shown with open circles. The solid line labeled Sum is what we would expect if Fos and Jun did not interact. The melts of the mixtures of Jun with the three potential dominant negatives (Jun + 0H-Fos, Jun + 4H-Fos, and Jun + A-Fos) are shown with closed points. The fitted curve through each of the data sets was used to calculate Tm as described previously. The Kd(37) for each mixture are shown in M (26). B, CD thermal melting curves at 222 nm of 1) A-Fos, 2) Jun, and 3) Jun + A-Fos. The solid line labeled Sum is what we would expect if Jun and A-Fos did not interact. C, CD thermal melting curves at 222 nm of 1) A-Fos, 2) VBP, and 3) VBP+A-Fos. The solid line labeled Sum is what we would expect if VBP and A-Fos did not interact.
|
We then examined the thermal stability of the Jun bZIP domain mixed
with three potential dominant negatives: the Fos leucine zipper without
the basic region or the Fos leucine zipper with one of two different
acidic extensions appended onto the N-terminus (Fig. 2A,
Table I). The mixture of Jun with Fos without a basic region (Jun + 0H-Fos) is twice as stable (G =
0.4 kcal/mol) as the Jun + Fos mixture, indicating that the basic regions are repulsive; a similar result was seen with the C/EBP basic region (26).
A surprising result was that the ellipticity at 6 °C for the Fos + Jun mixture was greater than for the mixture of Fos without the basic
region (0H-Fos) and Jun. This suggests that the basic regions are
helical in the absence of DNA, an observation that is not seen for
C/EBP (26). The addition of the acidic amphipathic extension to the
N-terminus of the Fos leucine zipper dramatically stabilizes the
interaction with Jun. Using the previously described acidic extension
(4H-Fos), we observe a 30-fold increase (
G =
1.8 kcal/mol) in the stability of the Jun bZIP domain. The new acidic
extension containing the single Asn
Leu change (A-Fos) is 3000-fold
more stable (
G =
4.6 kcal/mol) than the Jun + 0H-Fos mixture (Fig. 2, A and B, Table I). The
single amino acid change increased the heterodimer stability 2.8 kcal/mol.
The specificity of the interaction of A-Fos with additional bZIP domains was determined by CD thermal denaturation. The VBP bZIP domain and A-Fos were thermally denatured, either alone or together, and no interaction was observed (Fig. 2C). Similar results were obtained for the mixture of 4H-Fos and C/EBP (data not shown). This suggests that the acidic extension is only able to interact with the basic region if the leucine zippers themselves are physically interacting.
To determine if the new acidic extension containing the Asn Leu
change (A-) stabilized other bZIP basic regions or was specific for a
basic region containing a hydrophobic residue in the a position, we appended the new acidic sequence onto the C/EBP leucine zipper (A-C/EBP) and determined the thermal stability of mixtures with
C/EBP (Table I). C/EBP interacts similarly with both acidic extensions
with the following dissociation constants:
Kd (37) = 7 × 10
10
M for 4H-C/EBP and 8 × 10
10
M for A-C/EBP. In the context of the C/EBP leucine zipper,
the new acidic extension only contributes to a 0.1 kcal/mol increase in
stability, which is negligible. These data demonstrate that the Asn
Leu change produces a new acidic extension that interacts well with all
bZIP basic regions examined but prefers to interact with basic regions
containing a hydrophobic residue in the a position,
e.g. the Jun basic region.
A puzzling result from the thermal
denaturation experiments was the amount of ellipticity at 222 nm
indicative of -helical structure seen at low temperatures when the
samples are dimeric. The mixture of the Jun bZIP and Fos bZIP domains
(Jun + Fos) has more helicity than the mixture of Jun bZIP and the Fos
leucine zipper (Jun + 0H-Fos) (Fig. 2A). This suggests that
the basic regions of Fos and Jun are helical in the absence of DNA.
This result was not observed when similar experiments were done with C/EBP. The basic regions of the bZIP proteins GCN4 and C/EBP have been
shown to be nonhelical in the absence of DNA and to become helical when
bound to DNA (15, 16, 31). We measured the CD spectra from 200 to 250 nm of C/EBP and Fos:Jun in the absence and presence of
sequence-specific DNA (Fig. 3). CD ellipticity at 222 nm
is indicative of helicity. As reported earlier, we observe that the
C/EBP bZIP domain shows a 44% increase in helicity with the addition
of DNA (Fig. 3A) (31). In contrast, the Fos:Jun heterodimer
shows only a modest 10% increase in helicity after binding
sequence-specific DNA (Fig. 3B), an increase identical to
that reported earlier (32). Interestingly, the mixture of Jun with a
Fos zipper lacking the basic region (Jun + 0H-Fos) contains 40% less
helicity than the mixture of the Fos and Jun bZIP domains (Fig.
3B). This suggests that the Jun and Fos basic regions are
largely helical in the absence of DNA, unlike the C/EBP basic region,
although the basic regions are repulsive.
Inhibition of AP1 DNA Binding
Gel shift experiments were
undertaken to examine the number of molar equivalents of A-Fos that
would be needed to inhibit the DNA binding of a mixture of Fos and Jun.
Published data indicate that a Fos:Jun heterodimer binds DNA with a
Kd (25) = 2 × 1010
M (33). CD experiments presented in Table I indicate that A-Fos heterodimerizes with Jun with a
Kd (25) = 2.8 × 10
12 M. Therefore, an equimolar mixture of
Fos, Jun, and A-Fos should prevent Fos:Jun heterodimers from binding
DNA because of the preferred formation of the Jun:A-Fos heterodimer.
Fos, Jun, or a Fos + Jun mixture was incubated with a labeled 24-base
pair oligonucleotide containing a single AP1 site and tested for DNA
binding using a gel shift assay. Fig. 4A
shows that Fos does not bind AP1 DNA (lane 1) but that
Jun:Jun homodimers bind slightly (lane 2) and Fos:Jun
heterodimers bind well (lane 3). One molar equivalent of
A-Fos is able to totally inhibit Fos:Jun DNA binding (lane 6). The Fos leucine zipper without the acidic extension (0H-Fos) at equimolar conditions does not inhibit Fos:Jun binding (lane 4), while the previously described acidic extension (4H-Fos)
inhibits Fos:Jun binding only partially as indicated by the amount of
free probe remaining in the reaction. These data demonstrate that the mutation Asn
Leu has a dramatic effect on the ability of a DN to
prevent AP1 DNA binding. At equimolar concentrations, 0H-Fos and 4H-Fos
are not expected to inhibit Fos:Jun DNA binding, because they
heterodimerize with Jun with a lower affinity than Fos:Jun bound to
DNA. Indeed, Jun:0H-Fos shows a Kd (25) = 8.5 × 10
9 M and Jun:4H-Fos shows a
Kd (25) = 4.2 × 10
10 M.
Acidic extension appended to the Fos zipper
inhibits Fos:Jun DNA binding. A, the left panel
shows a gel retardation assay of Fos (lane 1), Jun
(lane 2), and Fos + Jun (lane 3), binding to an
AP1 (TGAGTCA) specific DNA probe that is 24 base pairs long. Inhibition
of Fos:Jun DNA binding was measured following the addition of a 1.1 molar equivalent of different DNs to the Fos:Jun complex. Lane
4, Fos + Jun + 0H-Fos; lane 5, Fos + Jun + 4H-Fos;
lane 6, Fos + Jun + A-Fos. A-Fos is able to inhibit Fos:Jun
DNA binding activity. The inhibition of Fos:Jun DNA binding is leucine
zipper-specific. The DNA binding of Fos:Jun was challenged with
different proteins containing the acidic extension appended to
different leucine zippers. A 1.1 molar equivalent of A-VBP (lane
7), 4H-CREB (lane 8), and A-CREB (lane 9)
was added to Fos + Jun and did not inhibit Fos:Jun DNA binding.
B, A-Fos does not inhibit the sequence-specific DNA binding
properties of CREB or VBP. Adding a 1.1 molar equivalent of 0H-Fos
(lanes 3 and 8), 4H-Fos (lanes 4 and
9), or A-Fos (lanes 5 and 10) does not
inhibit the binding of CREB or VBP to its target site DNA (lanes 2 and 7). C, A-Fos inhibits the DNA binding of AP1 purified
complex isolated from T cells. Lane 1 shows the DNA binding
of the AP1 purified complex on a AP1 DNA probe. Adding A-Fos to the AP1
complex (lane 2) inhibits AP1 binding, but adding A-VBP
(lane 3) did not inhibit AP1 DNA binding. Supershift
analysis using antibodies against Jun (lane 4) or against
NF-B (lane 5) added to the binding reactions shows that
this complex contains a Jun family member.
To examine the specificity of the A-Fos ability to inhibit a Fos:Jun mixture from binding DNA, we undertook two controls. The first control was used to determine whether the leucine zippers of 4H-Fos and A-Fos are critical for the inhibition of Fos:Jun DNA binding. Chimeric proteins were generated where the Fos leucine zipper was replaced with the VBP or the CREB leucine zipper. Fos:Jun DNA binding is not inhibited by A-VBP (lane 7), by 4H-CREB (lane 8), or by A-CREB (lane 9), indicating that the inhibition of Fos:Jun DNA binding is dependent on the Fos leucine zipper. The second control asked if A-Fos inhibits the binding of other bZIP proteins to DNA. To test this, we incubated equal molar quantities of the Fos DNs with two bZIP proteins, CREB or VBP. In Fig. 4B, we observe that 0H-Fos (lane 3), 4H-Fos (lane 4), and A-Fos (lane 5) do not inhibit the sequence-specific DNA binding of CREB (lane 2). The same results are obtained for sequence-specific VBP binding (Fig. 4B, lanes 8-10 and 7).
The ability of A-Fos to inhibit the DNA binding of a native AP1 complex
was determined (Fig. 4C). Purified AP1 protein complex, isolated from T cells, was bound to an AP1-specific DNA sequence (34).
A-Fos was able to totally inhibit DNA binding (lane 2), while the same acidic extension appended to the VBP leucine zipper was
not able to inhibit DNA binding (lane 3). The composition of
the AP1 purified complex was examined by performing an AP1 DNA binding
assay in the presence of supershifting antibodies directed against Jun
family proteins (lane 4). The AP1 complex is supershifted by
a Jun but not an NF-B antibody. These results demonstrate two
points: A-Fos is able to inhibit the DNA binding of a native AP1
complex, and the inhibition of DNA binding caused by A-Fos is leucine
zipper-dependent (Fig. 3C, lanes 3 and 4).
A transient transfection
assay in a human hepatoma cell line (HepG2) was employed to examine the
dominant negative properties of A-Fos. HepG2 cells were co-transfected
with the Jun transactivator and a CAT reporter gene containing a single
AP1 binding site. Jun is able to transactivate this promoter 10-fold
(Fig. 5A). Four different potential DNs were
tested for their ability to inhibit Jun transactivation at a 1:1 molar
ratio, an experimental condition where we tried to avoid overexpression
of the DN. These DNs are the Fos bZIP domain with the transactivation
domain deleted (bZIP-Fos), the Fos leucine zipper (0H-Fos), and the Fos
leucine zipper with the two acidic amphipathic extensions (4H- and A-) appended onto the N-terminus. Neither the Fos bZIP domain nor the Fos
leucine zipper, two possible DNs that could occur by the simple
deletion within the fos gene, were able to inhibit
transactivation under the experimental conditions used. 4H-Fos and
A-Fos inhibited Jun transactivation over 80%. Complete inhibition is
observed when a 3:1 molar ratio of the A-Fos to Jun transactivator is
used. The expression of the different DN proteins was checked by
Western blot (Fig. 5B) using the N-terminal FLAG epitope
present on each of the DNs. bZIP-Fos was overexpressed in HepG2 cells
compared with A-Fos, but it is not an efficient inhibitor of Jun
transactivation. 0H-Fos can hardly be detected on Western blot (data
not shown), but they can be seen at the same density as A-Fos using an
immunofluorescence assay (Fig. 5C). We think the 0H-Fos
epitope is proteolysed during the cell extract preparation, a problem
we have encountered previously (26).
Acidic extension to the Fos zipper inhibits
Jun transactivation. A, human hepatoma cells (HepG2) were
transiently transfected with three plasmids, a CAT expression plasmid
driven by a single AP1 cis element, the Jun transactivator,
and different dominant negatives. CAT activity was measured and
expressed as -fold activation ± S.D. relative to the activity of
the reporter plasmid alone. Jun transactivates a single AP1 site
approximately 8-fold. The Fos bZIP domain and the Fos leucine zipper
are not effective at inhibiting Jun-dependent
transactivation. The inhibition is leucine zipper-dependent. Replacing the Fos zipper with the CREB,
VBP, or C/EBP zippers does not affect Jun transactivation.
Transfections were carried out using 10 µg of the reporter containing
a single AP1 cis element, 3 µg of CMV Jun and 3 µg of
the different dominant negatives, except A-Fos labeled with a
star for which 9 µg were also transfected. B,
Western blot analyses of whole-cell extract prepared from HepG2
nontransfected cells (control) and cells transfected with different
DNs. The blot was incubated with an anti-FLAG antibody. C,
indirect immunofluorescence staining of FLAG epitope-tagged proteins
0H-Fos and A-Fos. HepG2 cells were transfected with 0H-Fos and A-Fos,
fixed, and stained with FLAG antiserum (left), Hoescht 33342 (center), or viewed through phase (right).
D, 4H-Fos and A-Fos do not inhibit C/EBP transactivation
properties. Transfections were carried out in HepG2 cells as described
in A, except that the reporter gene contains a single C/EBP
cis element, and the transactivator is C/EBP. C/EBP
is
able to transactivate the C/EBP-containing promoter and is not
inhibited by 4H-Fos or A-Fos. Transactivations were carried out using 10 µg of the reporter containing
the C/EBP cis element, 0.3 µg of mouse sarcoma virus C/EBP
, and 0.3 µg of 4H-Fos or 0.3 µg of A-Fos. E,
4H-Fos inhibits TPA-induced T cell activation. Jurkat cells were
transfected with 8 µg of the reporter plasmid p10 and either 0.5 or 2 µg of 4H-Fos expression vector and induced with phorbol 12-myristate
13-acetate and phytohemagglutinin for 18 h. CAT activity was
measured and expressed as -fold activation ± S.D. relative to the
activity of the reporter plasmid alone (noninduced).
To investigate whether A-Fos inhibition of Jun transactivation is dependent on the Fos leucine zipper (Fig. 5A), chimeric DNs were generated where the Fos leucine zipper was replaced by three different leucine zippers (C/EBP, CREB, or VBP). These chimeric DNs are not able to inhibit Jun transactivation, indicating that A-Fos is acting in a leucine zipper-specific manner.
To examine whether A-Fos is acting nonspecifically by inactivating
transcription, we determined whether A-Fos inhibited the transactivation of C/EBP, another bZIP transactivator. HepG2 cells
were transfected with a CAT reporter gene containing 1) a single C/EBP
binding site in the promoter and C/EBP
alone, 2) C/EBP
plus
4H-Fos, or 3) C/EBP
plus A-Fos (Fig. 5D). C/EBP
is
able to activate a single C/EBP cis element 7-fold. Either acidic extension appended onto the Nterminus of the Fos zipper does not inhibit C/EBP
transactivation, demonstrating that the dominant negative to AP1 does not inhibit the function of other bZIP
proteins.
The human Jurkat T cell line was used as a model to examine the effect of the Fos dominant negative (4H-Fos) on TPA-induced T cell activation (Fig. 5E). Incubation of Jurkat cells with phytohemagglutinin and phorbol 12-myristate 13-acetate phorbol ester (equivalent to TPA) results in the production of interleukin-2. The interleukin-2 promoter contains a TPA-responsive element that binds AP1 proteins and is a major target for the phorbol ester response. Jurkat cells were transfected with a CAT reporter gene containing three TPA-responsive elements. The addition of TPA results in a 10-fold activation of the reporter gene, and the co-transfection of 4H-Fos inhibits the TPA activation in a dose-dependent fashion. This result demonstrates that 4H-Fos is able to inhibit the transcriptional activity of a native AP1 complex in T cells.
DN to AP1 Inhibits Ha-ras-mediated Cellular Transformation More than Cell GrowthAP1 is an immediate early protein complex that plays an important role in the initiation of cellular growth (3). The activity of AP1 also is a critical downstream mediator of the proliferative effects of several oncoproteins, most notably members of the Ras family (35-37). To investigate the possibility that DNs to AP1 could be used to dissect further the role of AP1 in cellular growth and transformation, C1H10T1/2 mouse fibroblasts were stably transfected with the human Ha-ras oncogene and a 3-fold molar excess of vector DNA, bZIP-Fos, 0H-Fos, or A-Fos. The transfected cells were plated as described under "Experimental Procedures" to assay for focus formation as well as to assay for the ability of DN expressing cells to produce stable, G418r colonies (Table II). In most cases, the results from multiple, independent experiments were averaged, and the focus forming and colony forming efficiencies are expressed relative to the appropriate Ha-ras control. The results show that all three DN constructs inhibit both C1H10T1/2 cell growth and Ha-ras-mediated cellular transformation. The Fos bZIP domain lacking a transactivation domain, the type of DN previously used (20), reduced foci formation to 59% of the Ha-ras control while only inhibiting colony formation to 78% of controls. Using the same concentration of input DNA, 0H-Fos inhibits foci formation to 40%, while the acidic extension (A-Fos) decreases foci formation to 30% and inhibits colony formation to only 63%. Interestingly, when the average reduction in CE is expressed relative to the average inhibition in FFE, the A-Fos construct was shown to inhibit transformation 2-fold over the level anticipated from the observed impact of this protein on C1H10T1/2 cell growth. Reductions in focus formation similar to those seen with the DNs to AP1 were not observed when 4H-CREB and 4H-C/EBP were tested in Ha-ras transformation assays, indicating that the inhibition in foci formation is dependent on the Fos leucine zipper (Table II). We take these results to mean that A-Fos has the potential to be used as a highly specific DN to experimentally separate the various biological activities of AP1 in mammalian cells.
|
We have generated a DN protein termed A-Fos that inhibits the DNA
binding of AP1 in an equimolar competition. AP1 is a heterodimer composed of two bZIP proteins, a Fos family member and a Jun family member. A-Fos contains an acidic amphipathic protein sequence appended
onto the N-terminus of the Fos leucine zipper, and the acidic extension
physically interacts with the Jun basic region. The interaction of the
Jun basic region with the acidic extension extends the leucine zipper
dimerization interface into the basic region, thus preventing the basic
region in this heterodimeric structure from binding DNA (Fig.
6). The acidic protein sequence stabilizes the
interaction of the Fos zipper with the Jun bZIP domain 4.6 kcal/mol or
3000-fold. This increase in heterodimer stability makes this DN an
effective competitor in a stoichiometric competition. Gel shift
experiments indicate that A-Fos is indeed able to inhibit Fos:Jun DNA
binding in an equimolar competition experiment. Neither the Fos leucine
zipper alone nor a previously described acidic extension (4H-) appended
onto the N-terminus of the Fos zipper was able to inhibit Fos:Jun DNA
binding, demonstrating that the newly described acidic extension is
critical for the function of the A-Fos DN. The specificity of the
inhibition of DNA binding was assayed by replacing the Fos leucine
zipper with sequences obtained from two heterologous zipper proteins,
C/EBP and VBP. These proteins were not able to inhibit Fos:Jun DNA
binding, indicating that the specificity of the DN is derived from the leucine zipper and the stability of the complex from the acidic extension.
The ability of this newly designed DN (A-Fos) to inhibit the biological
function of AP1 was tested in a variety of assays. A co-transfection
assay using human hepatoma cells (HepG2) shows that A-Fos is able to
inhibit Jun-dependent transactivation of a promoter
containing a single AP1 cis element. When equal molar quantities of A-Fos plasmid DNA were added to the transfection, Jun-dependent transactivation was decreased to 20% of
controls. When a 3-fold molar excess of DN was added,
Jun-dependent transactivation was totally abolished. The
specificity of the inhibition was tested using two separate approaches.
The inhibition of Jun-dependent transactivation was tested
by appending the acidic extension onto several other leucine zipper
sequences that do not dimerize with either the Fos or Jun leucine
zippers. These potential DNs did not inhibit Jun-dependent
transactivation. The second approach was to test whether A-Fos inhibits
transactivation by the bZIP protein C/EBP. The transactivation of
C/EBP
is not inhibited by the A-Fos DN, demonstrating that A-Fos
only inhibits leucine zippers with which it can physically
interact.
The human Jurkat T cell line was used as a model to examine the effect of the Fos dominant negative on TPA-induced T cell activation. Incubation of Jurkat cells with phytohemagglutinin and TPA results in the production of interleukin-2. We showed that 4H-Fos is able to inhibit the transcriptional activity of a native AP1 complex in T cells, implying that it can be used as a reagent to study the the cascade of events leading to the the activation of the interleukin-2 gene.
A cellular transformation assay in C1H10T1/2 cells was utilized to explore whether A-Fos could inhibit Ras-dependent transformation. As an immediate early transcription complex, AP1 has been shown to be important for the initiation of cell growth (1, 38). In addition, the oncogenicity of retrovirally encoded variants of the c-Fos and c-Jun proteins has demonstrated a role for AP1 activity in cellular transformation. As a target for positive regulation by the mitogen-activated protein and Jun kinase cascades (35, 39), AP1 components have been implicated as downstream effectors in transformation by a number of oncogenes, and recent experiments have shown that transformation by the Ha-ras oncogene relies on the function of wild-type Jun (35, 37). Our results show that the DNs that inhibit AP1 DNA binding (A-Fos) are able to inhibit transformation better than DNs that are deleted for the transactivation domain (bZIP-Fos). The observation that A-Fos and bZIP-Fos have different effects on foci formation as compared with colony formation indicate that these reagents may be useful in unraveling the relationship between cell growth and transformation. Additional experiments in which stable cell lines expressing various levels of A-Fos are analyzed for growth and transformation properties will be necessary to establish the dose of A-Fos protein required to separate the biological effects of AP1 in mammalian cells (40).
Dominant negatives to the AP1 complex have been reported by several groups (41-44). These proteins act by interacting with endogenous AP1 family members and binding to DNA, which inhibits transactivation and transformation. A-Fos, the DN described in this manuscript, acts in a novel fashion; it heterodimerizes with endogenous AP1 family members, which inhibits DNA binding, thereby inhibiting transactivation and transformation. A-Fos, in both transactivation and transformation assays is more effective than bZIP-Fos or the Fos leucine zipper, the type of DNs used previously.
An additional advantage of the DN strategy presented here is the ability to explore repressive effects of a transcription factor. In different promoter contexts, some DNA-binding proteins can be either activators or repressors (41). The typical DN consisting of the bZIP domain could inhibit transactivation but not repression because the DNA site would remain occupied. Thus, previously described DNs are valuable for exploring the transactivation properties of a transcription factor but not the repression properties. The DNs described here should reveal any repressive properties of the AP1 complex in certain cell types and physiological conditions (40).
The experimental problem with these truncated proteins consisting of only the bZIP domain is the difficulty of demonstrating the mode of action. The DN to AP1 we have synthesized and characterized in this study forms heterodimers with Jun and prevents the normal AP1 family complex from binding DNA, creating a situation akin to ablating Jun family members' function in a cell. The inhibition of DNA binding should allow the demonstration in biological assays of the absence of DNA site occupancy using an in vivo footprint assay (25) and allow for the design of future experiments to identify target genes regulated by AP1.
We thank C. Peterson and J. Moitra for comments on the manuscript, M. Birrer and N. Colburn for insightful conversations, and Claude Klee for support and encouragement.