(Received for publication, March 31, 1997, and in revised form, May 28, 1997)
From the Department of Biochemistry, Recently, it was reported that the
activity of rabbit P450 1A2 is markedly increased at elevated salt
concentration (Yun, C-H., Song, M., Ahn, T., and Kim, H. (1996)
J. Biol. Chem. 271, 31312-31316). The activity
increase of P450 1A2 coincides with the raised The microsomal monooxygenase metabolizes a variety of endogenous
and xenobiotic compounds. This enzyme system includes cytochrome P450
(P450)1 enzymes, NADPH-P450
reductase, and phospholipids. Cytochrome b5 and
NADH-cytochrome b5 reductase may also contribute
to the electron flow (2). P450-dependent activities can be
reconstituted by mixing P450, NADPH-P450 reductase, and
phosphatidylcholine (PC) (3, 4). P450 and NADPH-P450 reductase seem to
be distributed randomly on the plane of membranes, and they interact
through lateral diffusion (5, 6). However, the organization of
constituent proteins in phospholipid membranes and their mechanism of
interaction are not fully understood yet. P450 and NADPH-P450 reductase
have been reported to interact by forming a functional complex for the
electron transfer (7). On the other hand, P450 is present in the
membrane in large excess over the reductase, with the molar ratios
ranging from 10:1 to 25:1, depending on treatment with inducers (8, 9).
Since NADPH-P450 reductase is the limiting component in
microsomes, different P450 enzymes must compete for the available
reductases.
Phospholipid molecules in the immediate vicinity of P450 in rabbit
liver microsomes have been known to be highly organized as compared
with those in bulk membrane (10). The functional properties of many
proteins in biological membranes, including the endoplasmic reticulum
membranes, seem to be closely related to the microenvironment provided
by membrane lipids (11, 12). It was proposed that the increase of P450
activities in a reconstituted system by the addition of phospholipid or
detergent was mainly due to the increased interaction between P450 and
NADPH-P450 reductase. It was also suggested that the interaction of
phospholipid with P450 may be necessary for maintaining an active
protein conformation and its ability to interact with NADPH-P450
reductase and necessary for efficient electron transfer (13).
The P450 1A2 is one of P450 enzymes that is induced by the polycyclic
aromatic hydrocarbon. It was demonstrated that the stimulated activity
of P450 1A2 by the high concentration of salt accompanies structural
change of P450 1A2 in the presence of phospholipid (14). It seems
worthwhile, therefore, to study the structural changes of P450 1A2
induced by phospholipid in detail to elucidate the relationship between
the structure and the activity. The catalytic reactions by cumene
hydroperoxide (CuOOH) are studied to evaluate the role of the protein
conformation in the catalytic function and to see how the environment
of the heme is influenced by the conformational change induced by
phospholipid and detergent. The relationship between phospholipid- or
detergent-induced conformational changes and activities of the P450 is
discussed. Here, the hydroperoxide system instead of NADPH-P450
reductase and NADPH is used to follow the change in the P450 1A2
conformation without a complicating effect of another protein,
NADPH-P450 reductase.
Sodium cholate, sodium deoxycholate, CHAPS, octyl
glucoside, CuOOH, and most of the phospholipids (DDPC, DUPC, DLPC,
DMPC, DPPC, DSPC, DAPC, DBPC, DLPE, DMPE, DPPE, DSPE, BBPC, BBPE, PS, PA, PI, PG, CL, and lyso-PC) were from Sigma. DCPC was obtained from
Doosan Serdary Research Laboratories (Englewood Cliffs, NJ). 7-Ethoxycoumarin, Triton X-100, Triton X-102, and Tween 20 were obtained from Aldrich. 7-Ethoxyresorufin and resorufin were obtained from Molecular Probes, Inc. (Eugene, OR). Emulgen 911 and 913 were
kindly provided by Dr. T. Shimada (Osaka Prefectural Institute of
Public Health, Osaka, Japan). Other chemicals were of the highest grade
commercially available.
The phospholipid vesicles or micelles were
prepared by hydrating a dry films in 100 mM potassium
phosphate buffer (pH 7.4), followed by sonication under nitrogen gas
while cooling on ice. Titanium particles and any residual multilamellar
structures were removed by centrifugation. The phospholipid
concentration was determined as described previously (15). The vesicles
were freshly prepared and added to the P450 solution just before the
activity assay and the spectral studies. It has been shown that P450
1A2 can be incorporated into preformed phospholipid vesicles (16).
P450 1A2 was purified from liver
microsomes of 5,6-benzoflavone-treated rabbits as described (17). The
P450 1A2 was electrophoretically homogeneous and had a specific P450
content of 17 nmol/mg protein. NADPH-P450 reductase was purified to an
apparent homogeneity from phenobarbital-treated rabbits as described
(18).
For the assay, two different approaches were taken to examine the
effect of phospholipid and detergent on the P450 1A2 activity using the
method described elsewhere with slight modification (14). For the
NADPH-P450 reductase-supported reactions, 0.5 nmol of P450 1A2, 0.6 nmol of NADPH-P450 reductase, 15 µg of phospholipid or detergent, and
an NADPH-generating system were mixed together. The volume of the
reaction mixture in potassium phosphate buffer (100 mM, pH
7.4) was 0.5 ml. Reactions were initiated by adding 2 µl of either
7-ethoxycoumarin (to 50 µM) or 7-ethoxyresorufin (to 5 µM) as the substrates.
When the reaction was supported by CuOOH, each incubate contained 0.5 nmol of P450 1A2 and 15 µg of phospholipid or detergent in addition
to the same concentrations of the substrates and phosphate buffer as
described above for the NADPH-P450 reductase-supported reactions. The
reaction was initiated by the addition of 2 µl of CuOOH (to 0.1 mM).
The reaction mixtures were incubated at 25 °C for 10 min, and the
products were estimated fluorometrically as was described elsewhere
for the assay of 7-ethoxycoumarin O-deethylation (19) and 7ethoxyresorufin O-deethylation (20).
Protein was assayed using a bicinchoninic acid procedure according to
the manufacturer's directions (Pierce). P450 concentrations were
determined by Fe2+-CO versus Fe2+
difference spectroscopy (21).
All spectroscopic experiments were done in 100 mM potassium phosphate buffer (pH 7.4) at 25 °C using
the methods described elsewhere with slight modification (14).
Fluorescence intensity was measured on a Shimadzu RF5301 PC
spectrofluorometer (Shimadzu Corp., Tokyo) in a thermostated cuvette.
Circular dichroic spectra were recorded on a Jasco J700
spectropolarimeter (Japan Spectroscopic, Tokyo) in a thermostated
cuvette. Blanks (buffer with or without phospholipid/detergent) were
routinely recorded and subtracted from the original spectra. Absorption
spectra were recorded with a Perkin-Elmer Lambda 16 spectrophotometer
(Norwalk, CT).
Stock solutions of P450 1A2 and NADPH-P450 reductase contained 20%
glycerol, but the final glycerol concentrations in all experiments were
kept below 0.2%, since glycerol affects the NADPH-P450 reductase-mediated reactions (22) and the protein structure (14,
23).
To investigate the effect of the head group and acyl
chain length of phospholipids on the enzyme activity of P450 1A2, we examined the P450 1A2-catalyzed reaction in the presence of different types of phospholipids. The enzymatic activity of P450 1A2 was quantified by measuring its ability to catalyze the
O-deethylation of 7-ethoxycoumarin and 7-ethoxyresorufin.
The activities toward both 7-ethoxyresorufin and 7-ethoxycoumarin, as
determined in the presence of CuOOH in place of the reductase and
NADPH, increased by 10-38% when BBPC or BBPE was added to the P450
1A2 solution (Table I). When lyso-PC was
added, there were no changes in enzymatic activities. In the presence
of negatively charged lipids, PS, PI, or PG, a substantial increase in
the P450 1A2 activities (38-86%) was observed. PA and CL, on the
other hand, inhibited P450 1A2-catalyzed reactions by 40-78%.
Table I.
Effect of phospholipid on the catalytic activities of P450 1A2
-helix content and
decreased
-sheet content. The presence of phospholipid magnified
this effect. Here, possible structural change of rabbit P450 1A2
accompanying the phospholipid-induced increase in its enzyme activity
was investigated by circular dichroism, fluorescence spectroscopy, and
absorption spectroscopy. Studies with the reconstituted system
supported by cumene hydroperoxide or NADPH showed that the P450 1A2
activities were found to be dependent on the head group and hydrocarbon
chain length of phospholipid. Phosphatidylcholines having short
hydrocarbon chains with a carbon number of 8-12 were very efficient
for reconstitution of the P450-catalyzed reactions supported by both
cumene hydroperoxide and NADPH. It was found that the phospholipid
increased the
-helix content and lowered the
-sheet content of
P450. Intrinsic fluorescence intensity is also increased in the
presence of phospholipid. The low spin iron configuration of P450 1A2
shifted toward the high spin configuration by most of the phospholipids
in the endoplasmic reticulum. Some synthetic phospholipids having short
hydrocarbon chains with a carbon number of 10-12 caused a shift in the
spin equilibrium of P450 1A2 toward low spin. The effect of detergents on the activity and conformation of P450 1A2 was also studied. It was
found that the addition of detergents to P450 1A2 solution increased
the enzyme activity of P450 1A2. Detergents also increased the
-helix content and lowered the
-sheet content of P450 1A2. Intrinsic fluorescence emissions also increased with the presence of
detergents. Octyl glucoside and deoxycholate caused a shift toward high
spin. On the other hand, cholate caused a shift toward low spin. It was
found that the activity increase of rabbit P450 1A2 coincides with the
conformational change including raised
-helix content. It is
proposed that the interaction with the phospholipid molecules
surrounding P450 1A2 in the endoplasmic reticulum is important for a
functional conformation of P450 1A2 in a monooxygenase system including
NADPH-P450 reductase.
Chemicals
Effects of Phospholipid and Detergent on the Catalytic Activities
of P450 1A2
Phospholipid
Activity
7-Ethoxycoumarin
O-deethylation
7-Ethoxyresorufin
O-deethylation
CuOOH
NADPH
CuOOH
NADPH
nmol product/min/nmol P450
None
0.136
0.120
0.080
0.182
BBPC
0.149
0.082
0.101
0.138
DCPC
0.211
0.295
0.152
0.511
DDPC
0.279
0.508
0.179
0.706
DUPC
0.237
0.372
0.163
0.599
DLPC
0.204
0.225
0.131
0.297
DMPC
0.185
0.103
0.106
0.173
DPPC
0.189
0.103
0.111
0.155
DSPC
0.188
0.108
0.110
0.146
DAPC
0.184
0.082
0.115
0.142
DBPC
0.185
0.096
0.118
0.144
Lyso-PC
0.129
0.239
0.083
0.495
BBPE
0.163
0.122
0.110
0.186
DLPE
0.188
0.101
0.096
0.173
DMPE
0.165
0.088
0.106
0.138
DPPE
0.189
0.130
0.101
0.129
DSPE
0.182
0.113
0.110
0.164
PS
0.231
0.179
0.149
0.260
PI
0.227
0.371
0.112
0.479
PA
0.072
0.304
0.048
0.411
PG
0.190
0.178
0.110
0.264
CL
0.041
0.212
0.018
0.351
Although mixed PC with various acyl chain lengths has been known to be an essential component of the microsomal P450 monooxygenase system, DLPC, a synthetic phospholipid, has been widely used for the reconstituted system (3, 4). We investigated the effect of the acyl chain length of phospholipids on the P450 1A2 activities using PC with a chain carbon number of 8-22 (Table I). PC is the major phospholipid in the endoplasmic reticulum. DCPC, DDPC, DUPC, and DLPC, having short hydrocarbon chains with a carbon number of 8-12, were very efficient for reconstitution of the CuOOH-supported reaction, and the activities increased by 50-124%. DMPC, DPPC, DSPC, DAPC, and DBPC having a chain carbon number of 14-22 were less efficient than PC with short hydrocarbon chains and showed the increase of activities by 32-48%. We also checked the chain length effect of PE, another major phospholipid in the endoplasmic reticulum. The effect of hydrocarbon chain length of PE is similar to that of PC. These results indicate that the chain length of both PC and PE exerts an effect on the P450 1A2-catalyzed reaction.
When the reconstituted system contained P450 1A2, NADPH-reductase, and NADPH, it was observed that the activities were also dependent upon the type of phospholipids (Table I). In the presence of BBPC, there was a 24-32% reduction in the activities of P450 1A2. When BBPE was added, there was no change in the activities. The addition of the negatively charged phospholipids to P450 1A2 solution caused the 1.4-3.1-fold increase in the activities. PI among them was the most efficient to reconstitute the P450 1A2-catalyzed reactions. These results indicate that certain anionic phospholipids may be important for the reconstitution. The effect of hydrocarbon chain length of PC on the P450 1A2 activities was also shown. When the synthetic PC with a chain carbon number of 8-12 was added to the P450 1A2 solution, the activities increased 1.6-4.2-fold. DDPC was the most efficient at reconstituting the P450 1A2 activities. The phospholipids with a chain carbon number of 14-22 were less efficient. Some of them inhibited up to 32% of the P450 1A2 activities. It is possible that an inefficient transfer of electrons from NADPH to P450 1A2 in the presence of PC vesicles with long hydrocarbon chains may cause the decreased catalytic activities.
We also investigated the effect of detergents on the P450 1A2 activities, because several detergents have been used for the P450 purification procedure and for the P450 reconstitution system (24, 25). The stimulatory effect of detergents with the exception of deoxycholate was routinely observed and provided about 1.2-1.9-fold enhancement depending on the head group type and chain length when CuOOH was present (Table II). With the reconstitution system containing P450 1A2, NADPH-P450 reductase, and NADPH in addition to detergent, it was observed that the activities increased up to 2.4-fold. In the case of cholate and octyl glucoside, there were no apparent changes of turnover numbers. These results may be due to the differential effect of each detergent on the conformation of P450 1A2 itself and also on the interaction of P450 1A2 with NADPH-P450 reductase.
|
It was reported that the absorption spectrum of P450 1A2 is sensitive to its own concentration and to the ionic strength of the solution (26). When the concentration of P450 1A2 was 1 µM and the concentration of potassium phosphate buffer (pH 7.4) was 100 mM, the spectrum was almost entirely originated from the absorption by the oxidized P450 1A2 in the presence of phospholipid or detergent without light scattering due to aggregation. Therefore, all of the spectroscopic studies were performed under these conditions.
The effect of phospholipid and detergent on the secondary structure of
the P450 1A2 was studied by CD in the far UV region. Fig.
1A shows the CD spectra of
P450 1A2 in 100 mM potassium phosphate buffer (pH 7.4) in
the presence of various types of PC and PE, the major phospholipids in
the endoplasmic reticulum (27). The CD spectra were curve-fitted by the
least squares method into the reference spectra obtained from five
proteins: myoglobin, lysozyme, ribonuclease A, papain, and lactate
dehydrogenase (28). Analysis of the CD spectrum for the P450 1A2 in the
absence of phospholipid and detergent yielded 32% -helix, 24%
-sheet, 23%
-turns, and 21% random structure (Table
III). When various types of PC and PE,
which have been known to have a high affinity on P450, were added, the
-helix content generally increased, while the amounts of
-sheet,
-turns, and random structure decreased (Fig. 1A and Table
III). The CD spectra of P450 1A2, obtained in the presence of synthetic
PC with a chain carbon number of 8-12, showed an increase of
-helix
content by 11-16% (Fig. 1A and Table III). However, the
effect of PC and PE with a chain carbon number of 8-12 was smaller
than that of these phospholipids with a chain carbon number of 14-22.
Overall, PE seems to be more effective in increasing the
-helix
content of P450 1A2 than PC. Lyso-PC was also efficient at increasing
the
-helix content and decreasing
-sheet and random structures of
P450 1A2.
|
To investigate the lipid specificity for the induction of secondary
structure of the P450 1A2, a comparison was also made between
zwitterionic (PC and PE) and anionic phospholipids (PS, PI, PA, PG, and
CL). Fig. 1B shows the CD spectra of P450 1A2 in 100 mM potassium phosphate buffer in the presence of different anionic phospholipids. For both PS and PA, the spectra with two minima
at 208 and 222 nm were observed, and the percentage of -helix was
calculated to be 42 and 48%, respectively (Table III). Although PS
induced a substantial increase in
-helical structure (Fig.
1B, Table III), it is less than what PC, PE, and PA brought about. The observed difference in
-helical content of the P450 1A2
upon interaction with different phospholipids is most probably due to
the head group specificity of the interaction. However, upon
interaction with other negatively charged phospholipids, PG, CL, and
PI, a decrease in CD spectral intensity of P450 1A2 was shown. It was
not possible in these cases to curve fit the CD spectra to estimate the
secondary structures using the reference spectra (28). It is possible
that the P450 1A2 aggregates in the presence of PG, CL, or PI with an
apparent molecular weight larger than its basic oligomeric structure of
P450 1A2 (29).
Fig. 1C shows the effect of detergents on the CD spectra of
P450 1A2. All of the detergents showed an effect on the CD spectra of
P450 1A2 similar to that of most phospholipids. All of the detergents
increased the -helix content by 14-20% and lowered
-sheet
content by 2-11% (Table III).
The intrinsic fluorescence of P450 1A2
reflects mainly the individual environments of its intrinsic
fluorophores, Trp, which are spaced evenly throughout its sequence
(30). The intrinsic fluorescence spectra of P450 1A2 obtained in the
presence of various types of phospholipids are shown in Fig.
2. The fluorescence intensity increased
in the presence of phospholipids, but there was no appreciable change
in the max value except for the case of PS (Fig.
2B). This indicates that the change brought about by
phospholipids reduces the quenching of the intrinsic fluorescence in
the P450 1A2, but otherwise the overall environment of the intrinsic
fluorophore appears to be remain unchanged. We did not observe any
further change in fluorescence intensity when the concentration of
phospholipid was increased (data not shown).
When PC with a chain carbon number of 8-12, which increased the P450 1A2-catalyzed reactions supported by both of CuOOH and NADPH, and DLPE were present, the intrinsic fluorescence intensity of the P450 1A2 increased by about 30-60% as compared with in the absence of phospholipid (Fig. 2A). Other PC and PE were less efficient in increasing the intrinsic fluorescence intensity. When DMPC, DPPC, DSPC, DAPC, or DBPC was added to the P450 1A2 solution, the intrinsic fluorescence spectra of P450 1A2 were similar to that of BBPC (data not shown). In the case of DMPE, DPPE, and DSPE, the spectra were similar to that of BBPE (data not shown). The addition of lyso-PC to the P450 1A2 solution caused a 2-fold increase in the intrinsic fluorescence intensity.
The effect of the negatively charged phospholipids on the intrinsic
fluorescence spectra of P450 1A2 was similar to those of the PC or PE
with a chain carbon number of 10-12. Interestingly, the addition of PS
to the P450 1A2 solution caused the typical red shift of the
max from 330 to 335 nm, which indicated that some
fluorophores were exposed to more hydrophilic environments.
The detergents also increased the fluorescence intensity of P450 1A2 at
max = 330 nm (Fig. 2C).
These results suggest that the stimulation of catalytic activity by phospholipid and detergent involves the increased secondary structure as well as the change of the overall conformation of P450 1A2.
Spin State of P450 1A2The P450 1A2 exists as a mixture of
high and low spin iron forms with corresponding absorption peaks at 395 and 414 nm, respectively. Fig. 3 gives
the absorption spectra of P450 1A2 complexed with different types of
phospholipids (Fig. 3, A and B) and detergents (Fig. 3C). The spectra obtained in the absence of
phospholipids and detergents are also given for comparison. When BBPC,
BBPE, or lyso-PC was present, the absorption spectra shifted toward the
high spin state in the spin equilibrium (Fig. 3, A and
D). But the spectra shifted toward the low spin in the
presence of synthetic PC or PE with a chain carbon number of 10-12.
When the negatively charged phospholipids, PS, PI, PG, and CL, were
added to the P450 1A2 solution, the absorption spectra shifted toward the high spin state (Fig. 3, B and E). Fig. 3,
C and F, show the effects of detergents on the
P450 1A2 spin state. Deoxycholate and octyl glucoside shifted the
equilibrium toward the high spin state, but cholate shifted the
equilibrium toward the low spin state. In contrast, no apparent changes
were noted in the spin equilibrium in the case of CHAPS, Tween 20, and
Lubrol WX.
We could not obtain the spectra in the presence of Triton X-100, Triton X-102, Emulgen 911, or Emulgen 913 because of the high light scattering of the detergents (data not shown).
The importance of the interaction of P450 with phospholipids in
microsomes was recognized early on (3, 4). PC has been known to be an
essential component in a reconstituted P450 monooxygenase system. The
effect of phospholipids on P450 activities seems to be the result of
their influences on the interaction between P450 and NADPH-P450
reductase for the electron transfer (31, 32). Although synthetic DLPC
and microsomal PC with varying lengths of acyl chain have been known to
be very effective in the reconstituted P450 monooxygenase system (4),
the effect of phospholipid on the P450 conformation without a
complicating effect of NADPH-P450 reductase is not fully understood
yet. Although some attempts were made to see the effect of phospholipid
on the P450 conformation (33-35), these were apparently overshadowed
by glycerol, which is usually used to stabilize the P450. The addition
of glycerol to the P450 1A2 solution induced an appreciable increase in
the -helix and decrease in the
-sheet content (14).
It was reported that P450 1A2-catalyzed reactions in the DLPC system proceeded at a rate more than twice that in the egg yolk PC vesicles, but no pronounced differences were seen in NADPH oxidase activity between the two types of reconstituted systems (36). The short synthetic phospholipid, DLPC, forms small micelles instead of bilayers (37). The membranous reconstitution system has been shown to exhibit different P450 activities than the micellar DLPC system (36). It was also observed that P450 has different affinities for the NADPH-P450 reductase in different phospholipid vesicles (16, 38). These findings suggest that each phospholipid may show the differential effect on the conformation of P450 itself and also on the interaction of P450 with NADPH-P450 reductase.
It was found that some detergents such as Emulgen 911, Emulgen 913, Triton N-101, Triton X-100, Triton X-114, CHAPS, and Lubrol PX could substitute for phospholipid in a reconstituted P450 system (24, 25). Ionic detergents such as cholate and deoxycholate were found to be rather ineffective. It was suggested that the role of phospholipid is physical rather than chemical and that both DLPC and certain detergents form micelles of appropriate dimensions to facilitate interaction between P450 and NADPH-P450 reductase. It was also reported that the negative charge of the membrane is important for the catalytic activity of P450 2B4 (33).
Recently, it has been shown that some typical P450 substrates, inhibitors, cytochrome b5, or high salts can cause a shift in the spin equilibrium of P450 1A2 toward high spin (24, 39, 40). It has also been reported that detergents, several P450 substrates, or alcohols influence the conversion of rabbit and rat P450 1A2 from a high to low spin iron configuration (26, 41-43). Interestingly, the effect of most of the phospholipids, except the PC with a small chain carbon number of 10-12, on P450 1A2 is very similar to their effect on P450 2B4. The effect includes the configurational change toward the high spin state (33). It has been reported that there is no correlation between the spin state and the catalytic activity of the rat P450s in reconstituted systems and that not all of the P450s exhibited a relationship between the spin state and reduction potential (42).
In the present study, we have investigated the possible correlation
between the increased enzyme activity and the conformational change of
the P450 1A2 when the protein interacts with various types of
phospholipids or detergents. The ability of the P450 1A2 to adopt the
-helix conformation has been proposed to be important for the high
enzymatic activity (14). This investigation established that the
conformation of P450 1A2 is highly dependent on the presence of
phospholipid and detergent. The results clearly show that all of the
phospholipids and detergents tested here can increase the
-helix
content and lower the
-sheet content of P450 1A2. Intrinsic
fluorescence emissions also increased with the presence of all of the
phospholipids (except for PS) and detergents. The low spin iron
configuration of P450 1A2 shifted toward the high spin configuration in
the presence of the phospholipids including PC and PE and detergents.
These phospholipid- or detergent-induced conformational change
coincided with elevated activity of P450 1A2-catalyzed reactions. These
structural studies revealed a strong interaction of the P450 1A2 with
phospholipid and detergent. It was impossible to determine whether the
high
-helix content of P450 1A2 induced by phospholipid and
detergent was the cause of the increased catalytic activity or if the
high
-helix contents were simply parallel phospholipid- or
detergent-induced phenomena without direct connection. Since the P450
is in close contact with phospholipids in the endoplasmic reticulum,
the conformation of P450 1A2 induced by phospholipid may actually be
the physiologically active form, which has high affinity for the
NADPH-P450 reductase. The stimulation of the activity by phospholipid
is consistent with the conformational change of P450 1A2 in addition to
the stimulated interaction between P450 and NADPH-P450 reductase. Changes in the lipid microenvironment are likely to control how one of
many P450s in the endoplasmic reticulum couples with a reductase
present in relatively small amounts. It is also possible that the
binding of a specific substrate alters the
-helix:
-sheet ratio of
P450, allowing coupling with a reductase.
The picture now emerging from this and previous studies (14) on the
interaction of the P450 1A2 and phospholipid is that the P450 1A2 binds
preferentially to phospholipids present in the membrane. The P450 1A2
with high -helix content and low
-sheet content appears to have a
high affinity to NADPH-P450 reductase. The conformation of P450 1A2
should be important for the enzymatic activity and the interaction with
NADPH-P450 reductase for an efficient electron transfer.