(Received for publication, December 24, 1996)
From the Department of Biological Sciences, Hedco Molecular Biology Laboratories, University of Southern California, Los Angeles, California 90089-1340
Escherichia coli DNA polymerase II
(Pol II) is a member of the group B, "-like" family of DNA
polymerases. Pol II is encoded by the damage-inducible dinA
gene and exhibits SOS induction under the control of Lex A
repressor. The polB gene was originally designated as the
structural gene for Pol II based on the absence of detectable Pol II
activity in cell lysates prepared from a strain containing the mutant
polB100 allele. Because polB and dinA
mapped at different chromosomal locations, it remained an open
question whether polB, in addition to lexA,
might be involved in regulating the expression of Pol II. We have
cloned and sequenced the polB100 mutant allele, including
adjacent surrounding sequences, and have expressed the mutant
dinA gene from Pol B100 on a high copy number plasmid. Our
sequence data reveal that polB and dinA
represent the same gene and that the original transduction
mapping of polB was inaccurate. We purified the mutant Pol
B100 polymerase and show that it retains 5 to 10% of the wild-type
level of polymerase activity. The Pol B100 mutation, Gly401
Asp401, is not located within any of the five conserved
domains that define group B polymerases. Pol B100 retains a wild-type
level of 3
5
exonuclease activity. We suggest that the normal
level of exonucleolytic proofreading associated with the mutant Pol B100 enzyme may explain the repeated failures, over the past two decades, to detect phenotypes in polB mutant strains.
Pol II1 was first isolated and
purified in 1970 from an Escherichia coli strain deficient
in Pol I (1). A Pol II mutant, polB100, was obtained by
mutagenizing cells and assaying for the absence of Pol II activity in
crude cell lysates (2). This "brute force" biochemical approach
used to obtain Pol II mutants was similar to that taken by DeLucia and
Cairns (3) to obtain Pol I mutants. Based on transduction mapping data,
Pol II was located clockwise of leu, at about 2 min on the
E. coli chromosomal map (4)(Fig. 1). The pol II mutant
allele, designated as polB100, showed no phenotype (4). The
mapping data for polB100 appeared consistent with the
approximate location of an independently isolated polB1
mutant (5).
In 1988, we observed that Pol II was induced as part of the SOS-regulon under control of the Lex A repressor (6). The structural gene for Pol II was subsequently found to coincide with dinA (7, 8), a DNA damage-inducible gene under SOS control (9). In contrast to polB, dinA was located counterclockwise of leu, mapping at about 1 min, between the genetic markers thr and ara (9, 10). DNA sequence analysis of the region surrounding dinA demonstrated that dinA was situated immediately adjacent to araD (Fig. 1)(7). The distance between dinA and polB is about 1 min on the E. coli chromosome.
To resolve the uncertainty concerning the relationship between polB and dinA, we cloned and sequenced the structural gene for Pol II and surrounding sequences from a strain (E. coli HMS83) containing the polB100 gene and purified and characterized the Pol II gene product. We refer to this product as Pol B100. Past difficulties in measuring a phenotype for the polB100 strain will be discussed in light of the biochemical properties of Pol B100.
HMS83 (polA1,
polB100, leu, cys
,
thy
) was obtained from Dr. R. Moses,
Oregon Health Sciences Center, and MC1061 (hsdR, araD139,
(araABC-leu)7679, galU, galK
lacX74, rpsL,
thi) was obtained from Dr. K. McEntee, UCLA. XL1-Blue
(supE44, hsdR17, recA1, ednA1, gyrA46, thi, relA1, lacF
[proAB+, lacIq, lacZDM15,
Tn10(tetR)] was purchased from Stratagene,
San Diego, CA. SG22099 (same as MC4100, but
clpA319::mini-kan) was provided by Dr.
Michael Maurizi, National Cancer Institute, National Institutes of
Health, Bethesda, MD. ZQ100 is similar to MC1061 but contains a
kanamycin insertion at the clpA locus from SG22099 and also
carries F
from XL1-Blue. We have shown previously that Pol II is
absent in MC1061 (11). Restriction enzymes were purchased from Promega
Corp., Madison, WI. Anti-Pol I antibody was a generous gift from Dr. L. Loeb, University of Washington. E. coli Pol III
,
complex and SSB were gifts from Dr. M. O'Donnell, Cornell University
Medical College, NY. Antibiotic concentrations were: ampicillin, 100 µg/ml; and kanamycin, 30 µg/ml. Genetic and molecular procedures
were standard (12, 13).
Genomic DNA from HMS83 was prepared using phenol/chloroform extraction followed by ethanol precipitation (13). The genomic DNA was digested with BamHI and packaged using a Lambda EMBL4 vector, from Clontech, Palo Alto, CA., and plated on E. coli Y1088 (Stratagene). A 2.4-kilobase wild-type polB (dinA) fragment from pHY400 (14) was used as the probe to isolate the polB100 gene from the HMS83 genomic library. Phage DNA from a positive clone was purified and digested with PvuII and PstI. A 5.0-kilobase fragment containing the polB100 gene, determined by Southern hybridization, was purified by agarose gel electrophoresis. The vector pT7T318U (Pharmacia Biotech Inc.) was digested with PstI and SmaI, and the 5.0-kilobase fragment was then subcloned into pT7T318U for sequencing. The entire polB gene and surrounding regions, spanning about 300 base pairs to each side, were sequenced using Sequenase (U. S. Biochemical Corp.).
Construction of polB100 Overexpression PlasmidSite-directed mutagenesis (15) of the wild-type
polB overexpression plasmid, pHY400, was used to construct
the polB100 overexpressing plasmid, pZQ83. A 25-mer
oligonucleotide with a single base substitution in the middle was used.
The primer sequence was
5-CATCACGTAGCCGCAGGGCTGGCG-3
, where ,
located 14 nucleotides from the 5
terminus, was incorporated in place
of C to generate the mutant primer sequence. After this plasmid was
constructed, the 2.4-kilobase polB fragment was resequenced to ensure that this was the only mutation in the gene.
The pZQ83 plasmid (AmpR) was transformed into strain ZQ100 (KanR, TetR), a polB deletion strain, so that the mutant enzyme Pol B100 could be purified in the absence of wild-type Pol II. We succeeded in purifying Pol B100 using the same purification procedure used previously for wild-type Pol II (14, 16). Although we had no difficulty obtaining a high expression level of Pol B100 in the presence of wild-type Pol II, we were unable to overexpress Pol B100 substantially in a Pol II null mutant background. Because the specific activity for nucleotide incorporation of Pol B100 was also extremely low, about 5-10% of that of wild-type, Western gels were run with a high-titer anti-Pol II antibody (11) to identify Pol B100 after each purification step. The concentration of Pol B100 was determined by comparing its band intensity in a Western blot with that of a known amount of wild-type Pol II. Total protein concentrations were determined using the Bradford assay kit from Bio-Rad, Hercules, CA.
DNA Polymerase and 3Extension of a 5-32P-labeled primer was used
as an assay for DNA polymerase activity. Two primer-template DNA
constructs were used: the first contained a 32P-labeled
15-mer annealed to an M13 DNA; the second is a gapped primer-template
DNA formed by annealing an unlabeled 15-mer downstream from the
32P-labeled primer to generate a 60-mer gap. The assays
were carried out as described previously (14, 16). Following a 5-min
reaction, the elongated primer extension product was resolved by
polyacrylamide gel electrophoresis. The integrated intensities of the
primer extension bands were measured using a PhosphorImager (Molecular Dynamics) to determine the polymerase activities for the mutant and
wild-type Pol II.
We assayed for potential trace contamination of Pol B100 by Pol I by
preincubating purified Pol B100 on ice for 5 min in the presence of
anti-Pol I antibody or by a mock preincubation serum in the absence of
antibody, followed by an additional 1-min preincubation at room
temperature. Primer elongation was then carried out at 37 °C for 5 min (14, 16), and the difference in elongation catalyzed by Pol B100 in
the presence and absence of anti-Pol I antibody served as a measure of
Pol I contamination. Klenow fragment (2 units) was used as a positive
control to show that the Pol I Ab was active; 1 unit catalyzes
incorporation of 10 nmol of nt in 30 min at 37 °C. Trace
contamination with Pol III was examined by incubating Pol B100 in the
presence of SSB or and
complex. Pol II and Pol III core, a
complex containing the
polymerase subunit, the
3
-exonuclease
proofreading subunit, and the
subunit, are both stimulated to carry
out highly processive DNA synthesis in the presence of
and
complex (17). However, SSB strongly inhibits Pol III core in the
absence of
,
complex, whereas Pol II activity is either
stimulated using primed M13 DNA or is unaffected by SSB using gapped
DNA (18).
3-Exonuclease activity was measured by degradation of either free
5
-32P-labeled primer molecules (15-mers) or degradation of
32P-labeled primer molecules annealed to M13 template DNA
(14, 16). The incubation conditions were similar to those used to measure primer elongation except that deoxynucleoside triphosphates were excluded from the reaction. A two-dimensional in situ
gel assay (following subsection) was used to determine the fraction of
exonuclease activity associated with the Pol B100 polypeptide and the
fraction of activity attributable to contaminating exonucleases.
A 5-32P-labeled 30-mer primer annealed to
M13 DNA contained a two-base mismatch at the 3
-primer terminus. The
labeled primer-template DNA was cast in a standard SDS-polyacrylamide
gel, and purified Pol B 100 was loaded on the gel. Electrophoresis,
carried out in the first dimension, resolved proteins having different
molecular weights. Removal of SDS by dialysis permitted protein
renaturation to occur, thereby allowing exonuclease digestion of
mismatched primer-template DNA in situ (19). Gel slices
containing product DNA were recast in a second gel, and primer
molecules of different lengths were resolved by electrophoresis carried
out in the second gel dimension. Integrated band intensities
corresponding to the presence of exonucleolytically degraded
32P-labeled primers of different lengths were quantified by
phosphorimaging.
We
introduced the wild-type dinA gene into HMS83 by growing
HMS83 to mid-log phase (A600, 0.5-0.6),
followed by infection with a lambda phage
(GG302) carrying wild-type
dinA. The HMS83 strain, containing lambda, carries both
wild-type Pol II and mutant Pol B100. A mixture of Pol B100 and
wild-type Pol II was purified from this strain by the standard Pol II
purification method (14, 16) and assayed for polymerase and
3
-exonuclease activities as described above.
The earliest Pol II mutants were assigned to the polB locus (4, 5), but recent evidence indicates that the damage-inducible dinA gene is the structural gene coding for Pol II (7, 8) and is located counterclockwise of polB by approximately 1 min on the E. coli chromosome (7, 10)(Fig. 1). A clearly defined role for Pol II in either replication or repair has yet to emerge; therefore, it is important to ask if polB might be a negative regulator of Pol II expression, or alternatively, if polB and dinA represent the same gene. To address this issue, we have investigated the relationship between polB and dinA and have purified and characterized the Pol B100 gene product and compared it with wild-type Pol II.
Pol B100 Has 10-20-Fold Lower Polymerase Activity Than Wild-type Pol IIThe strain HMS83, containing both the polB100
allele and an amber mutant in Pol I (polA1), was used to
isolate Pol II. Crude cell lysates prepared from HMS83 contained a
protein that cross-reacts with a high-titer, highly selective, anti-Pol
II polyclonal antibody (11) and shows the same migration on a Western
gel as purified wild-type Pol II (Fig. 2).
To isolate the gene coding for Pol II from HMS83, we used an
oligonucleotide containing the wild-type dinA sequence to
hybridize to HMS83 genomic DNA (see "Experimental Procedures"). We
cloned and sequenced the dinA gene from HMS83, containing
the polB100 allele, and found that it contained a single G
A base substitution at nucleotide position 1309 in dinA,
causing a substitution of Gly
Asp at position 401 (G401E) in Pol II
(7). The presence of a mutation in the structural gene encoding Pol II
in the polB100 mutant strain strongly suggests that
polB100 is this mutant dinA, i.e. polB is
identical to dinA. We will, therefore, refer to the mutant
Pol II protein (G401E) as Pol B100.
To study the effect of the Gly to Asp mutation on the enzymatic
properties of Pol II, we made a site-directed G A base substitution at nucleotide position 1309 in the dinA gene and placed it
on a high copy number plasmid. We purified Pol B100 using as an assay its cross-reactivity against anti-Pol II antibody in Western gels (Fig.
2).
It is necessary to overexpress Pol B100 in a strain devoid of wild-type Pol II activity to investigate the polymerase and proofreading properties of the mutant enzyme. For reasons that are unclear, we found previously that the level of overproduction of an exonuclease-deficient mutant of Pol II on a plasmid (16) was roughly 50-fold less in a Pol II null-mutant background than in a wild-type background. The level of overproduction of Pol B100 was found to be compromised in a similar manner. Nevertheless, we were able to purify Pol B100 from the Pol II null-mutant background and assay its polymerase and proofreading activities distinct from either Pol I or Pol III. Clp A, a member of the Clp family of proteases (20), was found to copurify with Pol B100. Therefore, a clpA deletion strain, ZQ100, was used for purification of Pol B100 (see "Experimental Procedures").
A standard 32P-labeled primer extension assay was used to
compare deoxynucleotide incorporation activities of Pol B100 with wild-type Pol II (Fig. 3). Wild-type Pol II was observed
to fill a 60-nt gap entirely, whereas Pol B100 added an average of only 2 or 3 nt with barely observable extension bands out to about 10 nt.
Pol B100 has approximately 5-10% of the polymerase activity of
wild-type Pol II, based on integration of the primer extension band
intensities when equimolar concentrations of each enzyme were present
in the primer extension assay (Fig. 3). 3
5
exonucleolytic degradation of the primers was observed for both polymerases, as shown
by the appearance of lower molecular weight bands below the original
primer band (Fig. 3). We will later present data showing that the
proofreading activities of Pol B100 and wild-type Pol II are similar,
if not identical.
Pol B100 has a 10-20-fold lower polymerase-specific activity than
wild-type Pol II and cannot be overexpressed significantly in a null
dinA mutant. Therefore, it is necessary to show that the low
polymerase activity is attributable to Pol B100 and not to the presence
of trace contaminants of either Pol I or Pol III. A primer extension
assay run in either the presence or absence of polyclonal Ab prepared
against Pol I was used to show that residual Pol B100 activity is not
caused by Pol I (Fig. 4). Weak primer extension
catalyzed by Pol B100 is unaffected by the presence of anti-Pol I Ab
(Fig. 4: compare lane 2 Pol I Ab, with lane 3, + Pol
I Ab). The 10-20-fold higher rate of primer extension catalyzed
by wild-type Pol II is also insensitive to the presence of anti-Pol I
Ab (Fig. 4, lanes 4 and 5), in contrast to the
strong inhibitory of effect of anti-Pol I Ab on Pol I Klenow fragment activity (Fig. 4, lanes 6 and 7).
A hallmark property of Pol II is its ability to interact with the processivity clamp protein in the presence of the
clamp loading
complex (17, 21, 22). The
,
complex are components of the Pol
III holoenzyme, a complex containing a Pol III core and the
sliding
clamp and five-protein subunit clamp loading
complex (23). Both Pol
II and Pol III core synthesize DNA processively in the presence of
,
complex and SSB (Fig. 5, lanes 5 and
8, respectively). Synthesis by either Pol II or Pol III core
alone is considerably less processive (Fig. 5, lanes 6 and
9), as shown by the presence of many more intermediate bands migrating between the unextended primer (Fig. 5, lane 1) and
60-nt full-length product DNA (Fig. 5, lanes 6 and
9). The mutant Pol B100 was stimulated to synthesize
full-length product DNA in the presence of
,
complex and SSB
(Fig. 5, lane 2), whereas synthesis was weakly distributive
in the absence of the processivity factors (Fig. 5, lane 3).
Thus, the mutant Pol B100 retained the ability to synthesize DNA
processively in the presence of
,
complex + SSB, despite its
compromised ability to synthesize DNA.
The presence of SSB in the reaction, in the absence of ,
complex, provides a means to distinguish between primer extension catalyzed by either Pol II or Pol III. Pol III core activity is inhibited strongly by SSB, whereas Pol II is insensitive to SSB on a
gapped DNA template (18). Primer extension products catalyzed by Pol
III core were significantly reduced in size and intensity in the
presence of SSB (Fig. 5, lane 10). In contrast to the strong inhibitory effect of SSB on synthesis by Pol III core, DNA synthesis by
wild-type Pol II was slightly stimulated in the presence of SSB (Fig.
5, lane 7). The key finding is that synthesis by Pol B100
was essentially unaffected by SSB (Fig. 5, lane 4). Thus, it
is unlikely that trace contamination by Pol III core is responsible for
the primer extension activity observed in the purified Pol B100
fraction.
-To demonstrate that 3-exonuclease activity is an
integral part of Pol B100, we ran an in situ two-dimensional
polyacrylamide gel (19), which allows identification of exonucleolytic
degradation products and the relative size of the 3
-exonuclease that
forms each product. A 32P-labeled primer annealed to ssM13
DNA was cast within a SDS-polyacrylamide gel prior to loading Pol B100.
The gel was run under denaturing conditions in the first dimension to
separate proteins having different molecular mass. Exonucleolytic
degradation of the primer molecules occurred in situ
following removal of the denaturant by diffusion (see "Experimental
Procedures"). Lanes cut from the original gel were run on a separate
gel, in a second dimension, to resolve primers of different lengths. A
detailed technical description of the in situ activity assay
is given by Longley and Mosbaugh (19).
The intense horizontal band observed on the gel corresponds to
nondegraded 30-mer primer molecules (Fig. 6).
Exonucleolytic degradation products, ranging from 29 to 23 nt, are
present as less intense bands running below the undigested primer band
(Fig. 6). The location of each of the product bands along the
horizontal direction is determined by the molecular mass of the
3-exonuclease.
Wild-type Pol II gives rise to a single product band (Fig.
6a) at a position corresponding to the 89.9-kDa molecular
mass of Pol II (7, 8). Two sets of primer degradation bands were observed for Pol B100 (Fig. 6b); one at the 89.9-kDa
location of Pol II, and the other generated by a contaminating
exonuclease having a molecular mass of about 30 kDa. When Pol B100 and
wild-type Pol II were mixed together and assayed in situ
(Fig. 6c), the banding pattern was identical to that for Pol
B100 (Fig. 6b). The mixing data support the idea that Pol
B100 contains an associated 3-exonuclease activity. We measured the
rate of primer degradation and found that Pol B100 has approximately
the same level of exonuclease activity as wild-type Pol II. Note that
in the gel shown in Fig. 6, the amount of wild-type Pol II used was 10 times greater than Pol B100.
Pol I Klenow fragment (Fig. 6d) was run in a separate
control lane to verify that its degradation products ran at a location consistent with the molecular mass (68 kDa) of the enzyme. We also
verified that the degradation products of Pol I ran at a molecular mass
of 109 kDa, but the bands were less intense because of a loss of signal
emanating from the removal of the 5-32P label by Pol
I-associated 5
3
exonuclease activity (data not shown).
The strain carrying polB100 was
originally characterized as having no detectable Pol II activity (4).
Although we have shown that Pol B100 purified from the
polB100 (HMS83) mutant strain contains a Gly401
Asp401 replacement causing a large reduction in
polymerase activity, there remains a remote possibility for the
existence of an additional mutation in HMS83 that might suppress or
inactivate Pol II. To investigate this possibility, we infected HMS83
with a lambda phage containing a wild-type copy of Pol II (see
"Experimental Procedures") and carried out the standard Pol II
purification.
A 32P-labeled primer annealed to M13 DNA was extended using
either 10 ng of Pol II purified from polB100
(HMS83(GG13)), which carried a single copy of the dinA
gene on lambda and a single chromosomal copy of polB100,
(Fig. 7, lane 2), or extended using 5 ng of
purified wild-type Pol II (Fig. 7, lane 3). DNA synthesis in
lane 2 results from the combined action of wild-type Pol II and mutant
Pol B100, present presumably at approximately equimolar concentrations.
Pol B100 makes a negligible contribution to the integrated band
intensities in lane 2 because the mutant polymerase is 10-20 times
less active than wild-type Pol II. The primer extension rates are
roughly similar in lanes 2 and 3, and because twice as much protein was
present in the lane 2 reaction, it follows that the specific activities
were similar for wild-type Pol II purified from the
-infected
polB100 and wild-type polB+ strains.
We, therefore, conclude that the 10-20-fold reduction in Pol II
activity in the polB100 mutant is caused by replacement of a
single amino acid, Gly401
Asp401, in the
dinA gene rather than by the presence of an additional mutation in a "putative" polB control gene.
In the 27 years since E. coli Pol II was discovered in 1970 (1), a specific role for this enzyme in DNA replication or repair has not been defined. The structural gene for Pol II was originally thought to be at the polB locus located clockwise of leu, at about 2 min on the E. coli chromosomal map (4, 5)(Fig. 1). However, it was subsequently shown that the structural gene is the SOS-controlled dinA gene, mapping at about 1 min (7, 8, 10). The difference in location left open the possibility that polB might act as a control gene for Pol II. In this study, we set out to clarify the relationship between polB and dinA and to characterize Pol II purified from the dinA gene of the polB100 mutant strain (4).
polB and dinA Are the Same GeneWe found a single G A
base substitution at nucleotide position 1309 in the dinA
gene cloned from the E. coli strain HMS83 carrying the
polB100 mutant allele. This mutation results in the substitution of Gly
Asp at amino acid position 401 in Pol II. Sequence analysis of the upstream region of dinA from HMS83
showed that the promoter element was located two nucleotides downstream from the 3
end of araD, as shown previously for wild-type
dinA (7), and a comparison of sequences surrounding
dinA from HMS83 found them to be identical to their
wild-type counterpart (7, 8). The observation that the
polB100 mutant strain HMS83 contains a mutant
dinA gene having the same surrounding sequences as wild-type dinA is conclusive evidence that polB and
dinA are identical.
The polB100 mutant was
originally identified by the absence of Pol II polymerase activity in
cell lysates prepared from the mutant strain (2). We made a
site-directed G A base substitution at nucleotide position 1309 in
the dinA gene, placed it on a high copy number plasmid, and
purified the mutant Pol II protein (G401E), which we refer to as Pol
B100, on the basis of its cross-reactivity against anti-Pol II antibody
in Western gels (Fig. 2). We found that Pol B100 contains about 5-10%
of the wild-type Pol II polymerase activity (Fig. 3). It is noteworthy
that the substitution of Gly401
Asp401
causes a dramatic reduction in nucleotide incorporation activity because this mutation is not located in any of the five conserved domains characteristic of the group B ("
-like") polymerases (24, 25). Replacement of the nonpolar Gly by the polar Asp probably does not
cause a dramatic change in the overall physical properties of Pol B100
compared with wild-type Pol II because the purification scheme used for
wild-type Pol II (14, 16) was used successfully in purifying the mutant
enzyme. However, a large change may be occurring in the active
conformation of the mutant polymerase, which will be interesting to
investigate by x-ray crystallography (26).
We showed that residual polymerase activity in the purified Pol B100 preparation was not attributable to contamination by either Pol I or Pol III. Incubation of Pol B100 in the presence of anti-Pol I antibody had no measurable effect on deoxynucleotide incorporation, demonstrating that Pol I was not responsible for the low level of polymerase activity in purified Pol B100 fractions (Fig. 4, lanes 2 and 3). Contamination by Pol III core was ruled out by showing that SSB inhibited primer elongation by Pol III core (Fig. 5, lanes 9 and 10) but not by Pol B100 (Fig. 5, lanes 3 and 4).
Both Pol III and Pol II were shown to carry out processive DNA
synthesis in the presence of the sliding clamp and clamp loading
complex (17, 21). The weak DNA synthesis catalyzed by Pol B100 was
primarily distributive in the absence of
and
complex (Fig. 5,
lane 3). However, despite its low polymerase activity, Pol
B100 was able to catalyze processive DNA synthesis in the presence of
,
complex + SSB (Fig. 5, lane 2), demonstrating that
Pol B100 retained Pol II-like biochemical properties. Pol II contains
an active 3
5
exonuclease proofreading activity (16). In marked
contrast to the strongly detrimental effect of the Gly401
Asp401 replacement on polymerase activity, the mutant
enzyme retained a normal level of 3
5
exonuclease proofreading
activity.
A
mutator phenotype resulted when Pol II was replaced on the E. coli chromosome by a proofreading-defective Pol II allele (27). We
found an increase in forward spontaneous mutations rates at
rpoB and gyrA loci in a Pol III antimutator
(dnaE915) background in normally dividing cells, and a
measurement of the spectrum of rpoB chromosomal mutations
revealed a G A transition hot spot specific to the
proofreading-defective Pol II exo
. There was also an
increase in reversion rates of F
(lacZ) base substitutions
and frameshifts in the presence of wild-type Pol III (27). In addition
to the mutator effects of Pol II in dividing cells, a loss of Pol II
proofreading also resulted in an increased reversion rate of an
F
(lacZ) frameshift mutation in nondividing cells (28).
These results document the importance of Pol II-associated proofreading
in controlling chromosomal and episomal mutagenesis in vivo.
Therefore, the difficulty in defining a phenotype for the
polB100 strain may be attributed to the presence of a
wild-type proofreading activity for the mutant Pol B100 (Fig. 6).
Although a well defined role for Pol II in replication or repair has
not yet been defined, it has now been established that Pol II is
involved in controlling mutation rates in vivo (27, 28). The
ability of Pol II to share polymerase accessory proteins with Pol III
to achieve high processivity offers the potential for Pol II to "take
over" for Pol III, perhaps to carry out specialized tasks requiring
moderate to high processivity that cannot be performed by Pol I. We
have found that the number of Pol II molecules per cell is in the range
of 30-50.3 Because there are only about
four Pol III molecules per cell (18), one might expect to find
conditions in which Pol II might compete favorably against Pol III for
access to the processivity clamp.
Pol II levels are induced by 7-fold following induction of SOS (6), whereas Pol III levels increase by only 2-fold, at most (6). Thus, in the presence of DNA damage, the increase in intracellular Pol II concentrations might enable the enzyme to recruit the processivity subunits to fill in repair patches numbering perhaps hundreds to thousands of bases. We speculate that Pol II can substitute for Pol III and vice versa in catalyzing long-patch repair. However, the small number of Pol III molecules might place stringent restrictions on the sole use of Pol III to carry out both semi-conservative replication and repair DNA synthesis. It may then fall to Pol II to "pick up the slack" in either or both processes, particularly when SOS is turned on.
Now that Pol II has been shown to play a role in the synthesis of chromosomal and episomal DNA in dividing and nondividing cells, it is timely and important to eliminate ambiguities arising from the different map locations of polB and dinA. Data presented in this paper, documenting that polB and dinA are the same gene, eliminate an uncertainty in the pathway governing the regulation of Pol II.
We express our appreciation to Dr. Kevin McEntee of UCLA for generous help in all aspects of this work and to Dr. McEntee and Dr. John Petruska, USC, for constructive comments concerning the manuscript. We are grateful for the efforts of Dr. Gary Trump, USC, for preparation of Pol II antibodies and for his continued interest in these studies.