(Received for publication, December 19, 1996, and in revised form, January 22, 1997)
From the Department of Genetics and Molecular
Biology, Institute for Virus Research, Kyoto University, Sakyo-ku,
Kyoto 606-01, Japan, the § Department of Molecular Biology,
Faculty of Science, Nagoya University, Chikusa-ku, Nagoya 464-01, Japan
and the ¶ Faculty of Pharmaceutical Sciences, Hokkaido
University, Sapporo 060, Japan
Ceramide has been proposed as a second messenger molecule implicated in a variety of biological processes. It has recently been reported that ceramide activates stress-activated protein kinase (SAPK, also known as c-Jun NH2-terminal kinase JNK), a subfamily member of mitogen-activated protein kinase superfamily molecules and that the ceramide/SAPK/JNK signaling pathway is required for stress-induced apoptosis. However, the molecular mechanism by which ceramide induces SAPK/JNK activation is unknown. Here we show that TAK1, a member of the mitogen-activated protein kinase kinase kinase family, is activated by treatment of cells with agents and stresses that induce an increase in ceramide. Ceramide itself stimulated the kinase activity of TAK1. Expression of a constitutively active form of TAK1 resulted in activation of SAPK/JNK and SEK1/MKK4, a direct activator of SAPK/JNK. Furthermore, expression of a kinase-negative form of TAK1 interfered with the activation of SAPK/JNK induced by ceramide. These results indicate that TAK1 may function as a mediator of ceramide signaling to SAPK/JNK activation.
Mitogen-activated protein kinase (MAPK)1 is a serine/threonine kinase that is commonly activated by growth factors and phorbol esters and is shown to play a crucial role in cell proliferation, differentiation, and early development (1-4). This classical MAPK (simply called MAPK here, also known as ERK) is activated by dual phosphorylation catalyzed by MAPK kinase (MAPKK), which is phosphorylated and activated by a serine/threonine kinase, generally called MAPKK kinase (MAPKKK). Recently, two novel members of MAPK-related enzymes have been identified (4). One subgroup is called stress-activated protein kinase (SAPK) or c-Jun NH2-terminal kinase (JNK) (5, 6), and the other is p38/MPK2 (7-9). MAPK, SAPK/JNK, and p38/MPK2 constitute the MAPK superfamily. It is generally thought that each subfamily of the MAPK superfamily is independently regulated by particular sets of extracellular stimuli and therefore may have distinct functions in various biological processes.
We have recently identified a novel member of mammalian MAPKKK, TAK1.
Several lines of evidence suggested that TAK1 functions in the signal
transduction pathways triggered by members of the transforming growth
factor- (TGF-
) superfamily (10). During the course of further
biochemical characterization of TAK1, we have found that TAK1 can be
activated in response to several stimuli that have been shown to
activate SAPK/JNK. Moreover, we noticed that these stimuli are known to
induce an increase in ceramide. Ceramide has recently emerged as a
second messenger molecule that induces multiple cellular responses such
as cell cycle arrest, differentiation, and apoptosis (11, 12). Most
recently, it has been reported that ceramide activates SAPK/JNK and the
ceramide-induced activation of SAPK/JNK is required for stress- and
ceramide-initiated apoptosis (13). This may highlight the role of
SAPK/JNK as a mediator of cellular responses induced by ceramide. But
the molecular mechanism by which ceramide induces the activation of
SAPK/JNK is unknown.
In this report, we first show that TAK1 is activated in cells treated with agents and stresses that induce the generation of ceramide. We then show that ceramide itself can activate TAK1. Furthermore, expression of an active form of TAK1 induced activation of SAPK/JNK and its activator, SEK1/MKK4. In addition, expression of a kinase-deficient form of TAK1 interfered with the activation of SAPK/JNK induced by ceramide. These results indicate that TAK1 acts as a mediator for the ceramide-induced activation of SAPK/JNK.
A673 human rhabdomyosarcoma cells, COS7 cells, and rat 3Y1 cells were cultured at 37 °C in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum.
Preparation of Recombinant ProteinsBoth kinase-negative
MPK2 (KN-MPK2) and wild-type SAPK were expressed as His-tagged
proteins and purified using a Ni2+-affinity column (14).
Both wild-type XMEK2/SEK1 and kinase-negative MAPK were expressed as
glutathione S-transferase fusion proteins and purified as
described (14). Recombinant c-Jun was expressed and purified as
described previously (14).
Wild-type and kinase-negative
forms of TAK1 cDNAs were subcloned into pSR-HA vector (15) to
generate pSR
-HA-TAK1 and pSR
-HA-KNTAK1, respectively. Rat
SAPK
, mouse SEK1, Xenopus MAPK, and Xenopus MAPKK cDNAs were also subcloned into pSR
-HA vector to generate pSR
-HA-SAPK, pSR
-HA-SEK1, pSR
-HA-MAPK, and pSR
-HA-MAPKK,
respectively. TAB1 (1-418) and a kinase-negative form of TAK1 were
previously expressed in the pEF vector (16). These plasmids were
transfected into COS7 cells by the LipofectAMINE method according to
the manufacturer's instructions (Life Technologies, Inc.).
The cells were lysed
in an extraction buffer (20 mM HEPES, pH 7.4, 150 mM NaCl, 12.5 mM -glycerophosphate, 1.5 mM MgCl2, 2 mM EGTA, 10 mM NaF, 2 mM dithiothreitol, 1 mM
Na3VO4, 1 mM phenylmethylsulfonyl fluoride, and 20 µg/ml aprotinin) containing 0.5% Triton X-100, and
lysates were clarified by centrifugation at 15,000 × g
for 10 min. For immunoprecipitation, the supernatants were incubated with a rabbit polyclonal antibody raised against COOH-terminal 19 amino
acids of TAK1 for 30 min at 4 °C. After the addition of protein
A-Sepharose (Pharmacia Biotech Inc.) beads, the lysates were incubated
for an additional 1 h. Immunoprecipitates were washed three times
with a wash buffer (20 mM Tris-Cl, pH 7.5, 500 mM NaCl, 10 mM
-glycerophosphate, and 5 mM EGTA) containing 0.05% Tween 20 and twice with
phosphate-buffered saline and subjected to in vitro coupled
kinase assays.
For immunoprecipitation of HA-TAK1, the cells were homogenized in an extraction buffer. For immunoprecipitation of other HA-tagged kinases, the cells were homogenized in the buffer containing 0.5% Triton X-100. Lysates were then clarified by centrifugation at 15,000 × g for 10 min. The supernatants were incubated with anti-HA monoclonal antibody (12CA5) for 30 min at 4 °C. After the addition of protein A-Sepharose beads, the lysates were incubated for an additional 1 h. The immunoprecipitates were washed as above and subjected to in vitro kinase assays.
Protein Kinase AssaysThe activity of immunoprecipitated
TAK1 was assayed by its ability to activate recombinant XMEK2/SEK1,
whose activity was assayed by its ability to phosphorylate recombinant
KN-MPK2. The immunoprecipitate was incubated with recombinant
XMEK2/SEK1 (0.5 mg/ml) in a solution (22 µl) containing 20 mM Tris-Cl, pH 7.5, 10 mM MgCl2,
and 100 µM ATP for 20 min at 30 °C. Then, KN-MPK2 (5 mg/ml, 3 µl) and [-32P]ATP (5 µCi) was added, and
the mixtures were incubated further for 10 min at 30 °C. The
reaction was stopped by the addition of Laemmli's sample buffer and
boiling. After electrophoresis, incorporation of phosphate into KN-MPK2
was detected by autoradiography and quantified by using an image
analyzer (Fujix BAS 2000). The activities of immunoprecipitated SAPK,
SEK1, MAPK, and MAPKK were assayed by their ability to phosphorylate
recombinant c-Jun, recombinant SAPK
, myelin basic protein, and
recombinant kinase-negative form of MAPK (KN-MAPK), respectively. The
immunoprecipitate was incubated with c-Jun (3.5 mg/ml), SAPK
(2 mg/ml), myelin basic protein (1 mg/ml), or KN-MAPK (1 mg/ml) in a
solution containing 20 mM Tris-Cl, pH 7.5, 10 mM MgCl2, and 100 µM
[
-32P]ATP (1 µCi) for 10 min at 30 °C. The
reaction was stopped by the addition of Laemmli's sample buffer.
Phosphorylated proteins were subjected to SDS-polyacrylamide gel
electrophoresis, visualized by autoradiography, and quantified by using
an image analyzer.
A673 human rhabdomyosarcoma cells were stimulated with TNF-,
IL-1, or anti-Fas antibody, and the immunoprecipitate with anti-TAK1 antibody was subjected to an in vitro coupled kinase assay.
This antibody specifically immunoprecipitated endogenous TAK1 (Fig. 1A). TAK1 activity increased in cells exposed
to these agents, peaked after 5-10 min, and declined gradually with
time (Fig. 1B). We next examined whether TAK1 could be
activated by treatment of cells with stresses. An expression construct
encoding epitope-tagged TAK1 (HA-TAK1) was transiently transfected into
COS7 cells. After treatment of the cells with UV irradiation or
sorbitol, TAK1 activity was determined in a coupled kinase assay. TAK1
activity increased within 5 min of exposure of cells to UV irradiation
and peaked at 10 min (Fig. 1C). Exposure of the cells to
hyperosmolarity with 0.5 M sorbitol also caused an increase
in TAK1 activity (Fig. 1C). TAK1 activity was maximal after
30 min, and the high level of activity was sustained for 60 min (Fig.
1C).
Because TNF- (17, 18), IL-1 (19, 20), anti-Fas antibody (21, 22),
and several stresses (13) were all known to induce an increase in
ceramide, we tested whether TAK1 could be activated by treatment of
cells with ceramide itself. COS7 cells were transfected with HA-TAK1
and treated with the membrane-permeable ceramide analogue,
C2-ceramide. HA-TAK1 was isolated by immunoprecipitation, and its activity was determined. TAK1 activity increased within 5 min
of exposure of cells to C2-ceramide, was maximal after
10-30 min, and declined with time (Fig. 2B).
C2-ceramide stimulated TAK1 activity in a
dose-dependent manner (Fig. 2C). This activation was not observed in immune complexes from cells transfected with an HA
epitope-tagged, kinase-negative form of TAK1 (Fig. 2A). The
endogenous TAK1 was also activated by treatment with
C2-ceramide (Fig. 2D). Taken together, these
results have demonstrated that ceramide is capable of inducing TAK1
activation.
We tested the effect of other lipids on the TAK1 activity. C2-dihydroceramide, which lacks the trans double bond at C-4-C-5 of the sphingoid base backbone, and 1,2-dioctanoyl-sn-glycerol, which is an activator of protein kinase C, failed to activate TAK1 activity efficiently (Fig. 2E). Arachidonic acid also failed to activate TAK1 (Fig. 2E). Thus, C2-ceramide specifically activates TAK1.
TAK1 was originally identified as a mediator of the signal
transductions triggered by the TGF- superfamily (10). Most recently, one of TAK1-binding proteins, TAB1, has been identified as an activator
for TAK1 (16). A truncated form of TAB1 lacking the TAK1-binding domain
(TAB1 (1-418)) was shown to act as a dominant-negative inhibitor of
TGF-
-induced gene expression (16). Overexpression of TAB1 (1-418)
almost completely suppressed the activation of TAK1 induced by TGF-
stimulation but had little effect on the ceramide-induced activation of
TAK1 (data not shown). These results may indicate that ceramide and
TGF-
activate TAK1 through TAB1-independent and
-dependent pathways, respectively.
A recent report of Verheij et al. (13) suggested the central
role of SAPK/JNK in the ceramide-induced signal transduction. We
therefore examined whether TAK1 participates in SAPK/JNK activation induced by ceramide. We have previously shown that a truncated form of
TAK1 lacking NH2-terminal 20 amino acids (TAK1N) is
constitutively active (10). The expression vector encoding TAK1
N was
co-transfected into COS7 cells with an expression vector encoding
HA-tagged SAPK/JNK or HA-tagged MAPK, and the activity of SAPK/JNK or
MAPK was determined in the immunoprecipitate obtained with anti-HA
antibody. Co-expression of TAK1
N induced activation of SAPK/JNK
(Fig. 3A) but not that of MAPK (Fig.
3B). Co-expression of TAK1
N with SEK1/MKK4 or MAPKK, direct activators of SAPK/JNK and MAPK, respectively, resulted in
activation of SEK1/MKK4 (Fig. 3C) but not that of MAPKK
(Fig. 3D). These results demonstrated that TAK1 is able to
activate the SEK1/MKK4
SAPK/JNK cascade but unable to activate the
MAPKK
MAPK cascade. Furthermore, when a kinase-negative form of
TAK1 was co-expressed, the C2-ceramide-induced activation
of SAPK/JNK was suppressed markedly (Fig. 3E), indicating
that TAK1 is required for SAPK/JNK activation induced by ceramide.
In this study we have shown that TAK1, a recently identified MAPKKK family molecule, is activated in cells treated with cytokines and stresses that are known to induce the generation of ceramide (13, 17-22). We have here further shown that ceramide itself is able to activate TAK1 (Fig. 2). TAK1 activation was not observed in cells treated with other types of lipids, including diacylglycerol and arachidonic acid (Fig. 2). These results thus suggest that TAK1 may participate in the signal transduction pathway initiated by ceramide.
In the present study, expression of a constitutively active mutant of TAK1 resulted in the activation of SAPK/JNK and its direct activator SEK1/MKK4 (Fig. 3, A and C). Expression of a kinase-negative mutant of TAK1 suppressed the activation of SAPK/JNK induced by ceramide (Fig. 3E). Therefore, TAK1 is necessary and sufficient for the activation of SAPK/JNK induced by ceramide. Although Raf-1, another member of MAPKKK family, has been shown to be a mediator of ceramide signaling to classical MAPK (23-25), a molecular link between ceramide and SAPK/JNK has been missing. Our present results have identified TAK1 as a crucial mediator of ceramide signaling to SAPK/JNK.
The mechanism of TAK1 activation in response to ceramide should be
elucidated. Several molecules have previously been reported as direct
targets for ceramide, including ceramide-activated protein kinase (26,
27), ceramide-activated protein phosphatase (28), protein kinase C-
(29), and Raf-1 (30). These molecules might lie upstream of TAK1.