Ligand Binding Specificities of the Two Mannose 6-Phosphate Receptors*

(Received for publication, August 28, 1996, and in revised form, October 28, 1996)

David E. Sleat Dagger and Peter Lobel Dagger §

From the Dagger  Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey 08854 and the § Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
Acknowledgments
REFERENCES


ABSTRACT

Two mannose 6-phosphate (Man-6-P) receptors (MPRs) direct the vesicular transport of newly synthesized lysosomal enzymes that contain Man-6-P from the Golgi to a prelysosomal compartment. In order to understand the respective roles of the Mr = 46,000 cation-dependent (CD-) MPR and the Mr = 300,000 cation-independent (CI-) MPR in lysosomal targeting, an assay has been developed that simultaneously measures the relative affinity of each MPR for multiple ligands. Glycoproteins containing Man-6-P were affinity-purified from the metabolically labeled secretions of mutant mouse fibroblasts lacking both MPRs. They were incubated with purified MPRs, and the resulting receptor-ligand complexes were immunoprecipitated by anti-MPR monoclonal antibodies coupled to agarose beads. Ligands were eluted with Man-6-P and then quantified following SDS-polyacrylamide gel electrophoresis. Saturating concentrations of CI-MPR resulted in the complete recovery of each Man-6-P glycoprotein in receptor-ligand complexes. Apparent affinity constants ranged between 1 and 5 nM for the individual species. Ligands precipitated by the CD-MPR appeared identical to those bound by the CI-MPR, with apparent affinity constants ranging between 7 and 28 nM. The binding affinities of the two receptors for different ligands were not correlated, indicating that the two MPRs preferentially recognize different subsets of lysosomal enzymes. In addition, saturating levels of CD-MPR resulted in the precipitation of only 50% of the total input ligands, suggesting that the CD-MPR binds a subpopulation of the Man-6-P glycoproteins bound by the CI-MPR. These results provide a biochemical mechanism, which, in part, may explain the interaction of the two MPRs with overlapping yet distinct subsets of ligands in vivo.


INTRODUCTION

Most newly synthesized lysosomal hydrolases are targeted to the lysosome by a mannose 6-phosphate (Man-6-P)1-dependent pathway (1, 2). Man-6-P residues are added to select N-linked oligosaccharides in a pre-Golgi compartment and are specifically recognized by Man-6-P receptors (MPRs), which direct intracellular trafficking of lysosomal enzymes to a prelysosomal compartment. The low pH of this compartment causes the complex to dissociate, releasing the lysosomal enzymes and allowing the MPRs to recycle back to the Golgi. In the absence of MPRs, most Man-6-P-containing glycoproteins are secreted from the cell. The Man-6-P-dependent pathway therefore allows for the redirection of newly synthesized proteins from the secretory pathway to the lysosomal targeting pathway.

Most mammalian cells contain two distinct MPRs. The cation-independent MPR (CI-MPR) is a Mr 300,000 transmembrane glycoprotein, which, in addition to targeting newly synthesized lysosomal enzymes, can bind extracellular ligands, resulting in their subsequent endocytosis and transport to the lysosome. The Mr 46,000 cation-dependent MPR (CD-MPR) is an oligomeric transmembrane glycoprotein that also participates in the intracellular targeting of lysosomal enzymes. Like the CI-MPR, the CD-MPR also cycles to the cell surface, but it does not bind extracellular ligands containing Man-6-P under physiological conditions in vitro.

It is not known why most mammalian cells contain two MPRs. Mutant mice expressing only one of the two MPRs are viable (3, 4, 5, 6) in the appropriate genetic background, while disruption of both MPRs results in early death (7), indicating that each receptor can functionally compensate for the loss of the other. However, such compensation is, at best, partial. Transgenic mice lacking the CD-MPR have elevated levels of urine Man-6-P glycoproteins and plasma lysosomal enzymes (5), suggesting that the CI-MPR cannot completely substitute for loss of CD-MPR function. In addition, cultured cells lacking either MPR exhibit increased secretion of multiple lysosomal enzymes (4, 5, 8, 9).

Incomplete functional compensation suggests a selectivity in MPR-ligand interaction for which the molecular basis remains to be elucidated. The most obvious possibility is that the MPRs simply have intrinsically different affinities for different Man-6-P glycoproteins, which is reflected by selective targeting in vivo. Thus, the loss of one MPR would result in mistargeting of all ligands that are specifically bound by that MPR, while targeting of ligands common to both MPRs would be directed by the remaining MPR.

This possibility can be directly addressed by comparing the affinities of the two MPRs for different Man-6-P glycoproteins. In this report, we describe an in vitro binding assay for the simultaneous measurement of the relative affinities of both MPRs for a wide range of phosphorylated lysosomal enzymes. We demonstrate that while both MPRs bind the same array of proteins, the respective affinity of each MPR for different phosphorylated glycoproteins is varied, and there is no correlation between the affinities of the MPRs for different Man-6-P glycoproteins (i.e. a relatively high affinity ligand for the CI-MPR is not necessarily a high affinity ligand for the CD-MPR). Importantly, we also demonstrate that the CD-MPR is capable of binding only a subset of the ligands bound by the CI-MPR. This provides a biochemical basis for a number of studies demonstrating that even high level expression of the CD-MPR cannot fully compensate for loss of the CI-MPR in cultured cells.


EXPERIMENTAL PROCEDURES

Materials

Hybridoma cells producing monoclonal antibodies reacting against the CI-MPR and the CD-MPR were kindly provided by Dr. Don Messner. Yeast phosphomannan was a generous gift from Dr. M. E. Slodki (U.S. Department of Agriculture, Peoria, IL). Other reagents were from Sigma and analytical grade unless stated otherwise.

Purification and Coupling of Anti-MPR Monoclonal Antibodies

Monoclonal antibodies were purified from the media of hybridoma cells grown in vitro by passage over a Protein A-Sepharose column and low pH elution (10). For immunoprecipitation of CI-MPR-ligand complexes, a mixture of monoclonal antibodies (DM86 and DM70) were coupled together to Affi-Gel 10 (Bio-Rad) using the manufacturer's protocol at 1.5 mg/ml bed volume of each. For the CD-MPR, DM22 was coupled at 3 mg/ml Affi-Gel 10. DM86 was also coupled for the immunodepletion of contaminating CI-MPR during the purification of CD-MPR (see below). Coupled antibodies were stored at 4 °C in phosphate-buffered saline containing 0.02% sodium azide.

Purification of MPRs

CD-MPR was purified using a modified version of the method of Sahagian et al. (11). 200 g of bovine liver acetone powder was homogenized in 4.5 liters of 0.2 M NaCl, 0.1 M sodium acetate, pH 6.0, 10 mM EDTA, in 0.5-liter batches for 30 s in a Waring blender. The suspension was centrifuged for 30 min at 7,500 × g at 4 °C, and then the pellet was resuspended in 4.8 liters of distilled water and recentrifuged. The pellet was resuspended in 0.4 M KCl, 50 mM imidazole, pH 6.7, 1% membrane grade Triton X-100 (Boehringer Mannheim), 1 mM phenylmethylsulfonyl fluoride, and solubilized at 4 °C for 16 h with gentle stirring. The material was centrifuged at 5000 × g at 4 °C for 60 min.

The CD-MPR was purified by affinity chromatography on pentamannosyl phosphate-Sepharose and immunodepletion of CI-MPR. Briefly, the supernatant was adjusted to 10 mM MnCl2 and applied to a 180-ml bed volume column of pentamannosyl phosphate immobilized on omega -aminoethyl-agarose (Sigma) as described (12). The column was washed with 5 volumes of column buffer (0.1 M NaCl, 0.1% Triton X-100, 50 mM imidazole, pH 6.7, 10 mM MnCl2, 0.02% sodium azide). The column was then connected in series to a 30-ml bed volume column of immobilized anti-CI-MPR DM86 antibody and eluted with 5 mM Man-6-P in the column buffer. Fractions containing the highest levels of protein according to the method of Bradford (13) were pooled, and Man-6-P was removed by three sequential dialyses for 15, 2, and 2 h against 40 volumes of column buffer without Man-6-P. The procedure was repeated using a second pentamannosyl phosphate affinity column (30-ml bed volume) and anti-CI-MPR mAB column (30-ml bed volume).

The pooled fractions containing CD-MPR were dialyzed for 3 × 1 h against 125 volumes of column buffer without MnCl2, aliquoted into small volumes, frozen in an ethanol-dry ice bath, and stored at -135 °C prior to use. The yield of CD-MPR was routinely between 5 and 10 mg/200 g of bovine liver acetone powder and was devoid of any CI-MPR detectable by (i) silver staining after SDS-PAGE; (ii) gel electrophoresis of the iodinated CD-MPR preparation, and (iii) by a functional assay (see "Results"). Purification of the soluble portion of the bovine CI-MPR from fetal bovine sera has been described previously (14). CD-MPR is not found in serum and did not therefore represent a potential contaminant of the CI-MPR.

Preparation of 35S-labeled Man-6-P Glycoproteins

Phosphorylated lysosomal enzymes were collected from TME6 embryonic mouse fibroblasts that lack both MPRs and hypersecrete most of their Man-6-P glycoproteins into the media (8). For development of the MPR binding assay, conditioned media from metabolically labeled cells was used as a source of Man-6-P glycoproteins. Cells (90-95% confluent) were incubated for 15 h in DME-F12 media containing 5% sCI-MPR-depleted fetal bovine serum, 10 µM methionine, and 0.1 mCi/ml [35S]methionine (DuPont NEN), and then chased for 4 h with the addition of unlabeled methionine to 0.1 mM (8). Inclusion of 5% receptor-depleted fetal bovine serum in the labeling media increased total incorporation of [35S]methionine by about 10-fold (data not shown). Fetal bovine serum was depleted of soluble CI-MPR by passage through a phosphomannan affinity column (14). Conditioned media were dialyzed for 3 × 2 h against 50 volumes of 0.1 M NaCl at 4 °C, aliquoted into 0.5-ml portions, and stored at -70 °C.

For determination of apparent MPR binding constants, Man-6-P glycoproteins were purified from conditioned media by affinity chromatography on immobilized sCI-MPR columns. Labeling was as described earlier but with a higher concentration of [35S]methionine (1.0 mCi/ml of media). Medium was collected and centrifuged at 5000 × g for 15 min. One-tenth volume of 10 × phosphate-buffered saline was added to the supernatant, which was then loaded onto a 2-ml bed volume Affi-Gel 10 affinity column containing 3 mg of sCI-MPR. The column was washed with 10 volumes of 1 × phosphate-buffered saline containing 5 mM beta -glycerophosphate and 1 mg/ml bovine serum albumin and eluted with the same buffer containing 10 mM Man-6-P. Eluate fractions containing the highest counts were pooled and dialyzed three times against 1 liter of 0.1 M NaCl, 5 mM beta -glycerophosphate for 15, 1, and 6 h before aliquoting and storage at -70 °C. Approximately 30% of the of the total labeled proteins in the TME6 conditioned media contained Man-6-P.

Iodination of Purified MPRs

CI-MPR and CD-MPR were iodinated as described (14), except the latter was dialyzed for 3 × 2 h against 1000 volumes of 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1% Triton X-100 prior to iodination.

Immunoprecipitation of Receptor-Ligand Complexes

For assay development, MPRs were incubated with 30 µl of dialyzed TME6 secretions (1.5 × 105 cpm) in a total volume of 200 µl containing 60 mM MES, pH 6.3, at 4 °C, 0.115 M NaCl, and 0.2% Tween (Pierce, Ultrapure grade). Optimization of this incubation buffer is described below. Incubations were at 4 °C for 15 h with rotation. Coupled antibodies were equilibrated by washing with 3 × 10 volumes of incubation buffer, and 30 µl of 1:1 slurry was added per reaction. Reactions were incubated for a further 4 h, and then beads were collected by centrifugation at 10,000 × g at 4 °C for 15 s. Pellets were washed once with 1 ml of incubation buffer. Man-6-P glycoproteins were eluted from the beads by boiling for 5 min in reducing SDS-PAGE loading buffer (60 mM Tris-HCl, pH 6.8, 10% glycerol, 2% SDS, 1% beta -mercaptoethanol, 0.002% bromphenol blue). Radiolabeled ligands were fractionated by 10% acrylamide SDS-PAGE.

Apparent binding constants were determined using affinity-purified Man-6-P glycoproteins (5 × 105 cpm) in 300-µl reactions with the following modifications. After incubation with MPR for 15 h, 200 µl of each reaction were transferred to microcentrifuge tubes containing 20 µl of antibody beads. The remaining incubation mix was used to measure total radiolabeled ligands in each sample. After 4 h of immunoprecipitation, the beads were pelleted, and the supernatant was reserved to measure the unbound ligands in each reaction. The beads were washed once with 1 ml of incubation buffer and repelleted. Man-6-P glycoproteins were specifically eluted from the antibody bead-bound MPRs by rotation at 4 °C for 60 min with 200 µl of 10 mM Man-6-P, which recovered 95-100% of bound ligands as judged by further extraction of the beads by boiling in SDS-PAGE sample loading buffer. The antibody beads were again pelleted, and the MPR-bound ligands were transferred to a fresh tube. SDS-PAGE sample loading buffer was added to 50 µl of each sample prior to boiling and electrophoresis. In order to prevent nonspecific absorption of these proteins, tubes were preblocked with 1% bovine serum albumin, 0.2% Tween-20 for 24 h at 4 °C prior to use.

Radiolabeled bands were visualized and quantified using a PhosphorImager 400 and ImageQuant 3.1 software. Samples of the total, bound, and unbound fractions from each binding reaction were routinely analyzed by SDS-PAGE. Recovery was generally 60-125% (data not shown). Background was measured from the corresponding position in lanes lacking the appropriate MPR (unless stated otherwise). As an internal standard, each precipitated glycoprotein is expressed as a percentage of the total at that molecular weight for each individual reaction. This was determined from lanes in which the total labeled material (removed prior to the addition of beads) was loaded directly for each MPR concentration.

Immunoprecipitation efficiency at each MPR concentration was determined using identical parallel reactions containing a tracer of iodinated MPR (20,000 cpm). Each sample was processed exactly as described above except that MPR concentration in each fraction (total, supernatant, and pellet) was determined by direct scintillation counting using a Cobra Autogamma counter (Packard, Downer's Grove, IL), which accurately reflected labeled MPR content as determined by gel electrophoresis and PhosphorImager analysis (data not shown). Binding data was corrected for incomplete immunoprecipitation of MPRs, assuming equivalent precipitation of free receptor and receptor ligand complexes.

Optimization of Incubation Conditions

The immunoprecipitation assay described here for the simultaneous measurement of MPR binding affinities and capacities failed to detect any ligands bound almost exclusively by one MPR (see "Results"). This was surprising, given previous findings (8, 9); therefore, we conducted the following series of control experiments to investigate a wide range of incubation conditions on MPR-ligand interactions. These were conducted at subsaturating receptor concentrations to detect potential effects on both affinity and specificity.

The secretions of MPR-deficient mouse fibroblasts contain a number of lysosomal enzymes, presumably including proteases and glycosidases. It was possible that these enzyme activities could be present under the ligand binding conditions and could influence MPR affinity and the molecular weight of the different lysosomal enzymes. However, no significant changes in total labeled protein content, total Man-6-P glycoproteins (determined by blotting with an iodinated sCI-MPR probe (14)), or the pattern of immunoprecipitated Man-6-P glycoproteins were observed under a variety of different experimental conditions (data not shown), including pH 5.5 and 7.5, and the presence or absence of EDTA, monoclonal antibodies, phosphatase inhibitor (beta -glycerophosphate), or protease inhibitors (leupeptin, pepstatin, and phenylmethylsulfonyl fluoride). The presence of manganese, while not affecting total protein content, had a significant effect on one ligand (band 9/10) and is discussed under "Results." These experiments confirmed that the monoclonal antibodies, MPR preparations, and ligand source were essentially free of protease, glycosidase, and phosphatase activities under the conditions tested. Sodium orthovanadate, a phosphatase inhibitor, inhibited MPR ligand binding, especially the CD-MPR, when present at 5 mM (data not shown).

Most studies comparing MPR-ligand specificities have used either MES or imidazole as a buffer and NaCl and MnCl2 as mono- and divalent salts, respectively. These conditions do not necessarily accurately model conditions in vivo and could potentially alter MPR specificity. We therefore evaluated a range of experimental conditions in terms of affinity and specificity of MPR-ligand interactions by comparing several buffers (MES, imidazole, PIPES, and MOPS, pH 6.5) and salts (sodium chloride, sodium acetate, potassium chloride, and potassium acetate). All proved to be equivalent for both MPRs in terms of ligand binding pattern and affinity. A number of detergents (Brij, CHAPS, Digitonin, SDS, Nonidet P-40, Tween-20, and Triton X-100) were also evaluated in terms of MPR specificity and affinity (data not shown). Since none affected the pattern of bound ligands, we routinely used 0.2% Tween 20, which gave the best signal:noise ratio in the assay.

Previous studies have generally used magnesium or manganese as a source of divalent cations for the CD-MPR in ligand binding experiments. We examined a number of divalent cations (data not shown) and found that the pattern of immunoprecipitated ligands was the same with all tested, with the exception of bands 9 and 10 (see "Results"). However, the total precipitated ligand varied; e.g. Mn2+ increased the total ligand bound by the CD-MPR approximately 1.5-fold compared with EDTA, while other divalent cations (10 mM Ca2+, Mg2+, Co2+, or Ni2+) had little effect. All divalent cations, especially Co2+, were slightly inhibitory for the CI-MPR. We also examined the effects of cytosolic levels of Ca2+ and Mg2+ on MPR-ligand interactions. Calcium was buffered using calcium chloride and the chelator BAPTA (Calbiochem) as calculated using the MaxChelator program (15). Free calcium concentrations ranging from 15 nM to 15 µM, in the presence or absence of 0.8 mM Mg2+, had little or no effect on the ligand binding specificity or affinity of either MPR (data not shown). The chelator dibromo-BAPTA (Calbiochem) selectively inhibited CD-MPR-ligand interactions, although this effect is probably unrelated to the divalent cation requirements of this receptor, since BAPTA and EDTA had no effect compared with reactions containing no chelating agents.


RESULTS

Previous studies have measured the affinity of both or either MPRs for a number of mannose 6-phosphorylated ligands, including monosaccharides, oligosaccharides, and some lysosomal enzymes (reviewed in Ref. 2). However, to date, a direct comparison of the affinities of the two MPRs for a wide range of naturally occurring ligands has not been conducted. This was the primary objective of this study, with the aim of shedding light on MPR targeting specificity in vivo.

Assay Specificity

Potentially confounding effects in the assay could include contamination of one MPR with the other, nonspecific binding of 35S-labeled proteins to the Affi-Gel or cross-reaction between the antibodies and the labeled proteins in the TME6-conditioned media. In Fig. 1, we address these possibilities and demonstrate that the antibodies are completely specific for their respective MPRs. Incubation of MPRs in the absence of coupled antibody does not result in precipitation of labeled proteins. A small quantity of the starting secretions nonspecifically coprecipitated with either coupled antibody alone or antibody incubated with the heterologous MPR. This demonstrates that the monoclonal antibodies are specific but also indicates some nonspecific entrapment. In view of this, a standard control for background quantitation of precipitated ligands was a reaction containing coupled antibody but no MPR.


Fig. 1. Specificity of MPR immunoprecipitation assay. Immunoprecipitation reactions contained a combination of MPRs (CI, 30 nM CI-MPR; CD, 100 nM CD-MPR) and coupled anti-MPR monoclonal antibodies (mAB) (CI, DM86 + DM70 anti-CI-MPR; CD, DM22 anti-CD-MPR) incubated with total labeled secretions of TME6 cells as a source of Man-6-P glycoproteins. Reactions containing CD-MPR also contained 10 mM MnCl2. A sample corresponding to (null)/1;12 of the TME6 secretions is shown in the Start lane. A slight difference in the mobility of a protein of 53 kDa is seen when bound by one MPR compared with the other. This is not seen when ligands are eluted with Man-6-P (see Fig. 3) and presumably represents distortion of the gel by the DM22 IgG heavy chain, which migrates at this approximate position. Exposure time was 16 h, and gels were printed in a color range of 1-200 machine counts.
[View Larger Version of this Image (74K GIF file)]


An additional concern was that binding of the antibody could influence MPR specificity. However, the pattern of precipitated ligands using soluble/solubilized MPR and coupled antibody was identical to the pattern of proteins detected using iodinated CI- or CD-MPR as an affinity probe in a blotting assay (14), indicating that this is not the case (data not shown).

pH Dependence of MPR-Ligand Interactions

The pH binding profiles at 4 °C of total phosphorylated lysosomal enzymes for both MPRs were determined using total labeled secretions from TME6 cells as a source of Man-6-P glycoprotein ligands (Fig. 2). Maximal binding of CD-MPR to phosphorylated lysosomal enzymes was at approximately pH 6.2 and dropped off steeply on either side of this peak. Binding was essentially abolished at pH > 8.2. In contrast, the CI-MPR had a similar pH optimum but retained binding activity at higher pH values; for example, at pH 8.0, the CI-MPR retained about 70% of its maximal ligand binding capabilities. Immunoprecipitation efficiency of both MPRs was unaffected by pH (tested at pH 5, 7, and 9; data not shown). These results are consistent with other studies of the effects of pH on the ligand interactions of the MPRs determined using different methods (16, 17, 18, 19, 20). The pattern of ligands bound by each MPR was identical at each pH, indicating that pH is not a modulator of relative MPR-ligand affinity (data not shown). Pohlmann et al. (9), using immobilized MPR affinity chromatography, also demonstrated that the pattern of ligands bound by each MPR was not influenced by pH.


Fig. 2. pH dependence of MPR-ligand interactions. MPR-ligand immunoprecipitation reactions were as described (see "Experimental Procedures") but contained 60 mM acetate (pH 4.8 or 5.3), 60 mM MES (pH 5.3, 5.8, 6.3, 6.8, or 7.3), or 60 mM HEPES (pH 7.3, 7.8, 8.3, or 8.8). MPR concentrations were 2 nM (CI-MPR) and 20 nM (CD-MPR). Reactions containing CD-MPR also contained 10 mM MnCl2. Error bars represent standard deviation of duplicate determinations (quadruplicate at overlapping pH values).
[View Larger Version of this Image (23K GIF file)]


Immunoprecipitation of MPR-Ligand Complexes

Development of the equilibrium binding assay was carried out using total labeled secretions of TME6 MPR-deficient embryonic mouse fibroblasts as a source of Man-6-P glycoproteins (Figs. 1 and 2). In contrast, apparent MPR binding affinities for different ligands were determined using Man-6-P glycoproteins that were further purified by affinity chromatography on immobilized sCI-MPR. This additional step removed components in the media that could potentially modulate MPR affinities (e.g. insulin-like growth factor II; reviewed in Ref. 21) and also allowed a known starting concentration of ligands to be used for definitive binding studies. Earlier studies (data not shown) suggested that the high concentration of coupled CI-MPR (~6 µM) would be sufficient to bind all Man-6-P glycoproteins in the media. For the purposes of this study, however, it was vital to ensure that no CD-MPR-specific ligands were being selectively lost. This was achieved by receptor binding assays on material that flowed through the affinity column (Ligand Source F, Fig. 3) or was bound and specifically eluted with Man-6-P (Ligand Source M, Fig. 3). In reactions containing CI-MPR and affinity-purified Man-6-P glycoproteins, most of the labeled ligands were immunoprecipitated as part of MPR-ligand complexes. No labeled proteins were immunoprecipitated when the column flow-through was a potential source of ligands, indicating that the affinity purification removed all available ligands for the CI-MPR. When the affinity-purified Man-6-P glycoproteins were used as ligands for the CD-MPR, approximately 50% were immunoprecipitated in receptor-ligand complexes. No labeled proteins were immunoprecipitated with the CD-MPR when the column flow-through was a source of potential ligands, indicating that CD-MPR-specific ligands were not lost during purification of the labeled Man-6-P glycoproteins. In support of this conclusion, the pattern of Man-6-P glycoproteins precipitated by both MPRs was identical when total labeled secretions of TME6 cells, rather than purified labeled Man-6-P glycoproteins, were used as a source of ligands (e.g. compare Figs. 1 and 3).


Fig. 3. Purified Man-6-P glycoproteins as a source of ligands for the MPRs. Reactions contained CI-MPR affinity column-purified Man-6-P glycoproteins (M) or column flow-through (F) as a ligand source, incubated with either 3 nM CI-MPR or 100 nM CD-MPR as indicated. Reactions containing CD-MPR also contained 10 mM MnCl2. Three samples were taken from each receptor-ligand binding reaction: the mixture before the addition of antibody beads (Total); the supernatant after incubation with antibody beads (Supnt.); and the Man-6-P eluate of the pelleted and washed beads (Pellet). Exposure time was 6 days, and gels were printed in a color range of 1000-10,000 machine counts.
[View Larger Version of this Image (96K GIF file)]


It is of interest that a purified Man-6-P glycoprotein of approximately 37 kDa (denoted band 9 in Fig. 4) appears to be converted to a lower molecular weight form (band 10) during the course of the incubation, and this conversion is more pronounced in the presence of manganese (Fig. 3; compare total fractions from reactions containing CI-MPR and reactions containing CD-MPR/manganese). This conversion is diminished when ligands are incubated with the CD-MPR in the absence of manganese (data not shown). This may represent the action of a selective, manganese-dependent protease or glycosidase. Interestingly, the processed form appears to have lower affinity for the two receptors. No changes in the relative levels of any other purified ligands were observed, although a band of approximately 42 kDa in the flow-through fraction is also diminished upon incubation with manganese (Fig. 3, compare lanes 1 and 5 with lanes 3 and 7).


Fig. 4. SDS-PAGE of 35S-ligands coimmunoprecipitating with increasing concentrations of the two MPRs. Ligand binding reactions and immunoprecipitation of MPRs was as described under "Experimental Procedures". After elution with Man-6-P, precipitated ligands were boiled for 5 min in reducing sample buffer and centrifuged, and the supernatant was analyzed by electrophoresis on a 10% polyacrylamide gel. After fixation and drying, the gels were exposed to PhosphorImager screens for 16 h and printed in a color range of 3-500 machine counts.
[View Larger Version of this Image (71K GIF file)]


SDS-PAGE of immunoprecipitated MPR-ligand complexes resolved at least 11 major 35S-labeled glycoproteins (Figs. 1, 3, 4). The overall pattern of Man-6-P glycoproteins bound by both MPRs is very similar and clearly comparable with the pattern of Man-6-P glycoproteins secreted by TME6 fibroblasts lacking both MPRs (8). (For the sake of comparison, we have adopted the same numbering system used in Ref. 8.) This result was unexpected, given the differences observed in ligands secreted by fibroblasts lacking either MPR (8, 9). In particular, we expected to observe selective binding of the CD-MPR to band 5, which is secreted predominantly in the absence of the CD-MPR and, by the same reasoning, selective precipitation of band 7 (cathepsin D) by the CI-MPR.

Determination of Apparent Receptor Dissociation Constants

It is possible that the pattern of precipitated Man-6-P glycoproteins varies with MPR concentration, depending upon individual receptor-ligand affinities. We examined this possibility by measuring receptor-ligand interactions as a function of receptor concentration. Representative gels are shown in Fig. 4. As observed previously (Fig. 1, lanes 3 and 7 from right)), the pattern of ligands bound by both MPRs is identical, indicating that both bind the same mannose-6-phosphorylated species. This banding pattern was also obtained with both MPRs when total labeled TME6 secretions were used as a source of Man-6-P glycoproteins (Fig. 1). Our failure to identify any ligands bound specifically by either MPR is in accordance with previous studies, which used high density coupled MPR affinity columns to compare binding specificities (9).

In addition to the MPR-bound fraction, labeled ligands in the total and unbound fractions were also routinely analyzed by SDS-PAGE (data not shown). Changes in the amounts of ligands in the bound and unbound fractions were complementary; for example, reactions containing high concentrations of CI-MPR contained very low amounts of ligands detectable in the unbound fraction.

Total precipitated Man-6-P glycoproteins are defined as the integrated volume in each gel lane from which the background (integrated volume in gel lanes from immunoprecipitations lacking MPR) is subtracted. Binding of total Man-6-P glycoproteins to each MPR is quantitated in Fig. 5. It is clear that ligand binding at the highest concentrations of CI-MPR and CD-MPR (in the presence of manganese) reaches a plateau. This does not result from saturation of the coupled antibody at higher MPR levels, since immunoprecipitation efficiencies remained essentially constant with increasing MPR concentration (Fig. 5). It therefore appears that higher MPR concentrations are saturating, i.e. interacting with all available ligands in each binding reaction. This does not necessarily preclude the existence of a population of very low (i.e. micromolar) affinity ligands for the CD-MPR that would not be detected in the assay described here. A saturating concentration for the CD-MPR in the absence of manganese could not be determined. Importantly, saturating concentrations of CI-MPR resulted in the precipitation of all input Man-6-P glycoproteins, whereas saturating CD-MPR, in the presence of manganese, bound only approximately 50% of the total labeled ligands. This indicates that the CD-MPR is capable of binding only a subset of the ligands bound by the CI-MPR (at least at relatively high affinity).


Fig. 5. Binding of total Man-6-P glycoproteins to MPRs. Upper panel, total counts (i.e. the whole gel lane) from duplicate gels as represented by Fig. 4 were determined using the PhosphorImager and plotted against MPR concentration after subtraction of background (0 nM MPR). Plots represent total Man-6-P ligands as a percentage of total counts in each reaction bound by the CI-MPR (bullet ), and the CD-MPR in the absence (open circle ) and presence (×) of 10 mM MnCl2 and are expressed as the mean ± S.D. Total counts for each reaction were determined individually and varied from 100 to 122% of input (CI-MPR), 95-119% of input (CD-MPR), and 77-90% of input (CD-MPR/manganese). Data are corrected for immunoprecipitation efficiency (lower panel) using an average value of 74 and 64% for the CD- and CI-MPR, respectively.
[View Larger Version of this Image (20K GIF file)]


In a sequential receptor binding experiment, the CI-MPR effectively bound the Man-6-P glycoproteins contained within the immunoprecipitation supernatant of a binding reaction containing saturating CD-MPR (data not shown). This control confirms that binding of the CD-MPR to a restricted set of the ligands bound by the CI-MPR is an intrinsic property of the CD-MPR and does not reflect ligand loss during incubation (e.g. by the action of contaminating protease or phosphatase activities in the CD-MPR preparation).

From Fig. 5, the apparent dissociation constants (Kd') of the CD- and CI-MPR for total Man-6-P glycoproteins were estimated to be >30 and 2 nM, respectively. The addition of MnCl2 decreased the Kd' of the CD-MPR for total phosphorylated lysosomal enzymes to approximately 11 nM.

In Fig. 5, binding of total Man-6-P glycoproteins with each MPR is quantitated, providing an overall picture of MPR-ligand interaction. An important question remains whether the CD-MPR binds 50% of all ligands bound by the CI-MPR or binds relatively more of some and less of others. In order to resolve this question, we quantitated MPR-ligand interactions and constructed binding curves, which allow determination of both the affinity and capacity of each MPR for the individual ligands.

Binding curves are presented in Fig. 6, from which apparent Kd values and relative binding capacities (Bmax) were estimated (Table I). (Bands 9 and 10, which do not remain in stable equilibrium during the incubation (see above), were excluded from this analysis.) Kd' values for binding to the CI-MPR were relatively similar for all ligands and varied between 1 and 5 nM. In the absence of manganese, Kd' values for binding to the CD-MPR were >25 nM. In the presence of manganese, Kd' values for the CD-MPR varied between 7 and 28 nM. The dissociation constants for the two MPRs for different ligands are comparable with those determined for individual lysosomal enzymes using different techniques (beta -galactosidase binding for the CI-MPR, 20 nM (22) and 25 nM (23) and to the CD-MPR, 270 nM (21); beta -glucuronidase binding to the CD-MPR, 0.28 nM (18) and 4.4-5.1 nM (20)).


Fig. 6. Binding of individual Man-6-P glycoproteins to MPRs. Each individual band depicted in Fig. 4 was quantitated, background-subtracted, and corrected for immunoprecipitation efficiency. Background was defined as the machine counts at the equivalent position in 0 nM MPR lanes. Results are the average of duplicate determinations and are plotted as a percentage of the total counts in each reaction for the individual bands.
[View Larger Version of this Image (37K GIF file)]


Table I.

Apparent dissociation constants and binding capacities of the MPRs for the individual ligands

Results are presented as the apparent dissociation constant ± S.D./Bmax as a percentage of total ± S.D. Binding constants are estimated from the receptor concentration required to precipitate half of the maximally bound ligand and are the averages of two independent experiments analyzed as described in the legend to Fig. 5.
Band CI-MPR CD-MPR CD-MPR/Manganese

1 1.5  ± 0.5 nM/101  ± 8% >80  ± 20 nMa/>33  ± 13% 11  ± 1 nM/44  ± 0%
2 2.2  ± 0 nM/87  ± 6% >70  ± 30 nM/>11  ± 1% 25  ± 2 nM/28  ± 3%
3 1.7  ± 0.2 nM/111  ± 11% >35  ± 15 nM/>30  ± 6% 13  ± 5 nM/75  ± 15%
4 2.0  ± 0.6 nM/78  ± 4% >30  ± 0 nM/>21  ± 2% 9  ± 4 nM/49  ± 15%
5 2.0  ± 0.6 nM/93  ± 3% >45  ± 15 nM/>23  ± 8% 25  ± 10 nM/56  ± 5%
6 2.1  ± 0.6 nM/111  ± 11% >65  ± 15 nM/>20  ± 4% 28  ± 2 nM/40  ± 2%
7 2.3  ± 0.3 nM/96  ± 9% >25  ± 5 nM/>22  ± 1% 7  ± 1 nM/37  ± 1%
8 4.4  ± 1.2 nM/83  ± 10% NDb 19  ± 7 nM/39  ± 6%
9 ND ND ND
10 ND ND ND
11 3.6  ± 1.6 nM/111  ± 0% >75  ± 5 nM/>16  ± 1% 23  ± 2 nM/36  ± 1%

a  The highest concentration of CD-MPR in the absence of Mn2+ did not appear to saturate ligand binding.
b  ND, not determinable.

Most, if not all, of the input ligands were associated with the CI-MPR at saturating concentrations, with Bmax values ranging from 78 to 111%. This MPR is therefore capable of binding most or all of the purified Man-6-P glycoproteins, with relatively similar affinities (1-5 nM). In contrast, saturating levels of CD-MPR bound a maximum of between 28 and 75% of the available ligands, depending on which particular ligand is examined. The CD-MPR, therefore, differs from the CI-MPR in that both its binding affinities and capacities are markedly different from one Man-6-P glycoprotein to another.

In Fig. 7, A and B, the binding capacity and apparent Kd values are compared for each ligand and are found to be unrelated for both MPRs. This confirms that the highest MPR concentrations were actually saturating for the high affinity ligands; if not, we would expect that at limiting MPR concentrations, ligands would compete for receptor binding, and thus the relative proportion of any given ligand bound by the MPR would be proportional to the affinity of the MPR for that ligand. The relative binding capacities of the MPRs for the different ligands were also uncorrelated (data not shown).


Fig. 7. Comparison of the apparent affinities and capacities of the MPRs for different ligands. Apparent dissociation constants for the CI-MPR and the CD-MPR (in the presence of manganese) for the different ligands, are plotted against each other. A, Kd' CI-MPR versus Bmax CI-MPR; B, Kd' CD-MPR versus Bmax CD-MPR; C, Kd' CI-MPR versus Kd' CD-MPR.
[View Larger Version of this Image (23K GIF file)]


In Fig. 7C, the apparent affinities of the two MPRs are compared for each ligand. Little or no correlation was observed, suggesting that the two MPRs have distinct sets of preferred ligands. This finding may, at least in part, provide a biological basis for observations suggesting that the two MPRs transport overlapping yet distinct subsets of lysosomal enzymes (8, 9).


DISCUSSION

Cell lines of embryonic mouse fibroblasts lacking both MPRs secrete most of their newly synthesized lysosomal enzymes containing Man-6-P (8, 9). In this study, we have used media conditioned by such cells as a source of Man-6-P glycoproteins to compare the interaction of immunoprecipitable MPRs with a wide range of ligands resolvable by SDS-PAGE. This approach has revealed that (i) the CI-MPR has greater affinity for the different ligands than the CD-MPR, (ii) the arrays of Man-6-P glycoproteins bound by each MPR appear to be identical, (iii) the two MPRs have different affinities for different Man-6-P glycoproteins, (iv) the affinities of the two MPRs for different ligands are not correlated, and (v) the CD-MPR is capable of binding only a subset of the ligands bound by the CI-MPR.

MPR-ligand interactions have been examined in a number of studies designed to clarify the roles played by the two receptors in lysosomal enzyme trafficking. The distinct functions of the two MPRs in this process, however, remain unclear. Two general approaches have been used to compare MPR function: first, directly measuring MPR-ligand binding in vitro; and second, indirectly measuring MPR-ligand interactions by examining lysosomal enzyme sorting in cell lines with altered expression levels of MPRs. In vitro measurements of MPR-ligand binding have made use of purified MPRs and a variety of potential ligands. In equilibrium dialysis experiments, both MPRs were found to have similar affinities for most of the saccharide and oligosaccharide analogs of Man-6-P tested with the exception that the CI-MPR, but not the CD-MPR, interacts with slime mold oligosaccharides that contain methyl-covered Man-6-P residues (17, 22). Also, the CI-MPR had considerably higher affinity for high mannose oligosaccharides containing two phosphomonoester residues than the CD-MPR. In a related study, the affinities of both receptors for a number of saccharides were measured by competition with beta -glucuronidase for immobilized MPRs (19). Again, the MPRs had very similar affinities for most ligands except oligosaccharides containing methyl-blocked Man-6-P residues.

Another in vitro study compared the binding of various phosphorylated oligosaccharides and lysosomal enzymes to immobilized MPRs (16). Only a third of the Man-6-P glycoproteins that bound to and eluted from immobilized CI-MPR were found to tightly bind to a column of immobilized CD-MPR at pH 7. Interestingly, another third of the CI-MPR ligands bound weakly to the immobilized CD-MPR, and the other third was unbound, suggesting that the ligands bound by the CI-MPR represent three categories of ligands for the CD-MPR (high, low, and very low affinity). It is worth stating that when the binding was conducted at pH 6.3, 90% of the input ligands bound to the immobilized CD-MPR. One possible explanation is that under optimal conditions, even weak ligands are bound by the high concentration of immobilized receptor, thus obscuring even marked differences in affinity. Thus, in our study, it is possible that the subset of ligands bound by submicromolar concentrations of CD-MPR are equivalent to the high affinity ligands detected previously.

The molecular basis for the differences in ligand binding between the two MPRs also remains to be elucidated. One possibility is that determinants at the amino acid level could influence the affinity of different ligands for either MPR. For example, cathepsin L contains polypeptide components that decrease its affinity for the CI-MPR (24). However, this does not seem a likely explanation for the failure of the CD-MPR to interact with the ligands bound by the CI-MPR, since it binds a subset of the same polypeptides. It is possible, however, that changes in protein conformation could play a role in modulating MPR affinity, perhaps by limiting steric availability of Man-6-P residues. Perhaps the most likely possibility is that lysosomal enzymes exist in a number of mannose 6-phosphorylation isoforms that are bound preferentially by the two receptors. Indirect support for this possibility comes from carbohydrate analysis of phosphorylated lysosomal enzymes secreted by cells deficient in either MPR, which has revealed subtle differences in phosphorylation, depending on which MPR is lacking (25).

The respective roles of the MPRs have also been examined by monitoring the effects of altering the expression of either or both MPRs on the sorting of lysosomal enzymes in cultured cells. Many of these studies have used mouse L cells, which are deficient in the CI-MPR but contain endogenous CD-MPR. These cells secrete 60% of their newly synthesized beta -glucuronidase but secrete only 5% when transfected with the CI-MPR (18). However, even when overexpressing high levels of transfected CD-MPR, these cells still secrete 30-40% of the beta -glucuronidase (18, 20). Similar results have been obtained with cathepsin D (26, 27). In other studies, CD-MPR function has been blocked by adding anti-CD-MPR antibodies to cultured cells (28). Such immunodepletion in CI-MPR-deficient cells results in increased secretion of lysosomal enzymes, demonstrating that the CD-MPR has some capacity for targeting. However, immunodepletion of the CD-MPR in cells containing the CI-MPR has no effect on lysosomal enzyme sorting (28). Finally, lysosomal enzyme targeting has also been studied in transgenic mouse fibroblasts lacking both or either MPRs (8, 9). Secretion of lysosomal enzymes was considerably more pronounced in the absence of the CI-MPR than the CD-MPR, although loss of both MPRs resulted in the greatest missorting (9). In addition, while the pattern of secreted Man-6-P glycoproteins in the absence of either MPR was very similar, there were subtle differences depending on which MPR was absent, suggesting that the two MPRs target overlapping but distinct populations of ligands in vivo (8, 9).

Our finding that the CD-MPR binds only a subset of the ligands bound by the CI-MPR provides a rational explanation for the failure of the CD-MPR to compensate for loss of the CI-MPR. Our results also predict that the CI-MPR should be capable of fully compensating for loss of the CD-MPR in vivo. However, while the CI-MPR, in general, has a greater capacity for substituting for CD-MPR function in terms of lysosomal enzyme secretion than vice versa (9), it is clear from the studies described previously that this prediction is not borne out. This indicates that either MPR targeting specificity in vivo is regulated by more than just receptor-ligand affinity or that MPR-ligand affinities in vivo differ from those measured here. The fact that we observe in vitro binding of the same ligands to both MPRs, whereas in cultured cells, several Man-6-P glycoproteins are secreted only in the absence of one MPR (8, 9) only serves to further illustrate the potential complexities of the targeting process. Nonetheless, our results quantitatively demonstrate that the two MPRs have different affinities and capacities for Man-6-P glycoproteins in vitro, which is very likely to influence their targeting specificities in vivo.

Finally, the ligand binding assay described here should prove to be a valuable analytical tool in the analysis of Man-6-P glycoproteins bound preferentially by either MPR. Identification of these ligands and characterization of their respective carbohydrate structures will provide a direct approach for understanding the selectivity in MPR-ligand interactions at the molecular level, which, in turn, should shed light on the respective functions of the MPRs in vivo.


FOOTNOTES

*   This work was supported by National Institutes of Health Grant DK45992. The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
   To whom correspondence should be addressed. Tel.: 908-235-5360; Fax: 908-235-4850.
1    The abbreviations used are: Man-6-P, mannose 6-phosphate; Kd, dissociation constant; Kd', apparent Kd; MPR, Man-6-P receptor; CD-, cation-dependent; CI-, cation-independent; sCI-, soluble cation-independent; BAPTA, 1,2-bis-(o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid); PAGE, polyacrylamide gel electrophoresis; CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid; MES, 4-morpholineethanesulfonic acid; MOPS, 4-morpholinepropanesulfonic acid; PIPES, 1,4-piperazinediethanesulfonic acid.

Acknowledgments

We thank Jill Remmler for excellent technical assistance and Drs. Aaron Shatkin and Bernard Hoflack for critical review of the manuscript.


REFERENCES

  1. Kornfeld, S. (1992) Annu. Rev. Biochem. 61, 307-330 [CrossRef][Medline] [Order article via Infotrieve]
  2. Hoflack, B., and Lobel, P. (1993) in Advances in Cell and Molecular Biology of Membranes (Tartakoff, A. M., Storrie, B., and Murphy, R. F., eds), Vol. 1, pp. 51-80, Jai Press, Inc., Greenwich, CT
  3. Filson, A. J., Louvi, A., Efstratiadis, A., and Robertson, E. J. (1993) Development 118, 731-736 [Abstract/Free Full Text]
  4. Koster, A., Saftig, P., Matzner, U., von Figura, K., Peters, C., and Pohlmann, R. (1993) EMBO J. 12, 5219-5223 [Abstract]
  5. Ludwig, T., Ovitt, C. E., Bauer, U., Hollinshead, M., Remmler, J., Lobel, P., Ruther, U., and Hoflack, B. (1993) EMBO J. 12, 5225-5235 [Abstract]
  6. Wang, Z. Q., Fung, M. R., Barlow, D. P., and Wagner, E. F. (1994) Nature 327, 464-467
  7. Ludwig, T., Eggenschwilrer, J., Fisher, P., D'Ercole, A. J., Davenport, M. L., and Efstratiadis, A. (1996) Dev. Biol. 117, 517-535 [CrossRef]
  8. Ludwig, T., Munier-Lehmann, H., Bauer, U., Hollinshead, M., Ovitt, C., Lobel, P., and Hoflack, B. (1994) EMBO J. 13, 3430-3437 [Abstract]
  9. Pohlmann, R., Wendland, M., Boeker, C., and von Figura, K. (1995) J. Biol. Chem. 270, 27311-27318 [Abstract/Free Full Text]
  10. Ey, P. L., Prowse, S. J., and Jenkin, C. R. (1978) Immunohistochemistry 15, 429-436
  11. Sahagian, G. G., Distler, J. J., and Jourdian, G. W. (1982) Methods Enzymol. 83, 392-396 [Medline] [Order article via Infotrieve]
  12. Distler, J., and Jourdian, G. W. (1987) Methods Enzymol. 138, 504-509 [Medline] [Order article via Infotrieve]
  13. Bradford, M. M. (1976) Anal. Biochem. 72, 248-254 [CrossRef][Medline] [Order article via Infotrieve]
  14. Valenzano, K. J., Kallay, L. M., and Lobel, P. (1992) Anal. Biochem. 209, 156-162 [CrossRef]
  15. Bers, D., Patton, C., and Nuccitelli, R. (1994) Methods Cell Biol. 40, 3-29 [Medline] [Order article via Infotrieve]
  16. Hoflack, B., Fujimoto, K., and Kornfeld, S. (1987) J. Biol. Chem. 262, 123-129 [Abstract/Free Full Text]
  17. Tong, P. Y., and Kornfeld, S. (1989) J. Biol. Chem. 264, 7970-7975 [Abstract/Free Full Text]
  18. Watanabe, H., Grubb, J. H., and Sly, W. S. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 8036-8040 [Abstract]
  19. Distler, J. J., Guo, J., Jourdian, G. W., Srivastava, O. P., and Hindsgaul, O. (1991) J. Biol. Chem. 266, 21687-21692 [Abstract/Free Full Text]
  20. Ma, Z., Grubb, J. H., and Sly, W. S. (1991) J. Biol. Chem. 266, 10589-10595 [Abstract/Free Full Text]
  21. Nissley, P., and Kiess, W. (1991) in Molecular Biology and Physiology of Insulin and Insulin-like Growth Factors (Raizada, M. K., and LeRoith, D., eds), pp. 311-324, Plenum Publishing Corp., New York
  22. Tong, P. Y., Gregory, W., and Kornfeld, S. (1989) J. Biol. Chem. 264, 7962-7969 [Abstract/Free Full Text]
  23. Jourdian, G. W., Li, M., and Distler, J. J. (1987) Protides Biol. Fluids Proc. Colloq. 35, 395-398
  24. Lazzarino, D., and Gabel, C. A. (1990) J. Biol. Chem. 265, 11864-11871 [Abstract/Free Full Text]
  25. Munier-Lehmann, H., Mauxion, F., Bauer, U., Lobel, P., and Hoflack, B. (1996) J. Biol. Chem. 271, 15166-15174 [Abstract/Free Full Text]
  26. Lobel, P., Fujimoto, K., Ye, R. D., Griffiths, G., and Kornfeld, S. (1989) Cell 57, 787-796 [Medline] [Order article via Infotrieve]
  27. Johnson, K. F., and Kornfeld, S. (1992) J. Biol. Chem. 267, 17110-17115 [Abstract/Free Full Text]
  28. Stein, M., Zijderhand-Bleekemolen, J. E., Geuze, H., Hasilik, A., and von Figura, K. (1987) EMBO J. 6, 2677-2681 [Abstract]

©1997 by The American Society for Biochemistry and Molecular Biology, Inc.