Mmot1, a New Helix-Loop-Helix Transcription Factor Gene Displaying a Sharp Expression Boundary in the Embryonic Mouse Brain*

(Received for publication, January 24, 1997, and in revised form, April 14, 1997)

Nicoletta Malgaretti Dagger §, Ombretta Pozzoli Dagger §, Alessandro Bosetti Dagger , Anna Corradi Dagger , Sonia Ciarmatori Dagger , Maddalena Panigada Dagger , Marco E. Bianchi Dagger , Salvador Martinez par and G. Giacomo Consalez Dagger **

From the Dagger  Department of Biological and Technological Research (DIBIT), San Raffaele Scientific Institute (HSR) and  Department of Genetics and Microbiology, University of Milan School of Biology, I-20132 Milan, Italy and par  Institute of Morphological Sciences, University of Murcia School of Medicine, E-30071 Murcia, Spain

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES


ABSTRACT

Several genetic factors have been proven to contribute to the specification of the metencephalic-mesencephalic territory, a process that sets the developmental foundation for prospective morphogenesis of the cerebellum and mesencephalon. However, evidence stemming from genetic and developmental studies performed in man and various model organisms suggests the contribution of many additional factors in determining the fine subdivision and differentiation of these central nervous system regions. In man, the cerebellar ataxias/aplasias represent a large and heterogeneous family of genetic disorders.

Here, we describe the identification by differential screening and the characterization of Mmot1, a new gene encoding a DNA-binding protein strikingly similar to the helix-loop-helix factor Ebf/Olf1. Throughout midgestation embryogenesis, Mmot1 is expressed at high levels in the metencephalon, mesencephalon, and sensory neurons of the nasal cavity. In vitro DNA binding data suggest some functional equivalence of Mmot1 and Ebf/Olf1, possibly accounting for the reported lack of olfactory or neural defects in Ebf-/- knockout mutants. The isolation of Mmot1 and of an additional homolog in the mouse genome defines a novel, phylogenetically conserved mammalian family of transcription factor genes of potential relevance in studies of neural development and its aberrations.


INTRODUCTION

A number of transcription factor genes regulate cell identity in specific body regions, both in invertebrates and vertebrates (1). In vertebrates, the Hox genes control identity along the body axis and provide positional cues for the developing neural tube, particularly the rhombencephalon and spinal cord from the branchial area to the tail. Conversely, development of the anteriormost body domain, including the metencephalic, mesencephalic, and prosencephalic territories (2), has remained relatively obscure in invertebrates and vertebrates alike. Indeed, the molecular specification of compartments or subdivisions in the vertebrate forebrain is still a matter of debate (3).

Specifically, regarding morphogenesis of the metencephalon-mesencephalon boundary, a recent breakthrough came with the identification of the mouse En-1 and En-2 genes, which were cloned based on their homology to the Drosophila segment polarity gene engrailed (4). En-2 homozygous mutant mice created by homologous recombination in embryonic stem cells are viable and exhibit a patterning defect in the cerebellum (5, 6). In contrast, En-1 homozygous mutant mice die at birth and show a deletion of most of the colliculi and cerebellum (7). Likewise, the Wnt and Pax families of genes have been implicated in cerebellar patterning by means of genetic or neurobiological studies (8, 9), whereas the Fgf-8 gene has been shown to play a critical role in the induction of the isthmic organizing center (10).

Despite these and other relevant advances, mostly based on developmental mechanisms conserved from Drosophila to vertebrates, our knowledge of rostral central nervous system differentiation in general and of metencephalic-mesencephalic specification in particular remains fragmentary to date, and many other as yet unidentified regulatory genes may at different times play a role in various cell fate specification or terminal differentiation processes.

To help elucidate some of the molecular mechanisms underlying the fine subdivision and differentiation of primary brain structures during midgestation brain development, our group set out to screen for developmentally regulated genes restricted in their spatial and temporal expression domains within the embryonic head. This was achieved through a modification (11)1 of a PCR-based2 differential screening technique named RNA fingerprinting (12, 13).

Among other embryonic central nervous system genes of regulatory significance found in this way, we have isolated a new helix-loop-helix (HLH) transcription factor gene, Mmot1 (metencephalon-mesencephalon-olfactory transcription factor 1), differentially expressed along the anteroposterior axis, displaying a sharp anterior expression boundary within the diencephalon as well as a high level specific expression in the sensory portion of the olfactory epithelium.

HLH transcription factors are nuclear proteins that bind DNA as homo- or heterodimers. HLH transcription factors have been subdivided into various subfamilies (14), and their role has been recognized in Drosophila neurogenesis and sex differentiation as well as vertebrate myogenesis (15) and neurogenesis (16, 17). The newest subclass of HLH proteins identified so far includes two virtually identical, independently cloned genes: a mouse gene named Ebf (early B-cell factor) (18) and a rat gene named Olf1 (olfactory-neuronal transcription factor) (19) as well as their Drosophila homolog (collier) (20). A specific feature of this subfamily is that its members lack the basic domain found upstream of the first alpha  helix in basic HLH transcription factors, which mediates DNA binding. In Ebf, the establishment of DNA-protein interactions is mediated by an N-terminal domain, inclusive of a zinc finger element, whereas the HLH domain appears exclusively involved in dimerization (21).

The present paper describes the isolation, genetic characterization, in situ expression studies, and in vitro DNA binding properties of Mmot1, a new member of the Ebf/Olf1-like subclass of HLH transcription factors.


EXPERIMENTAL PROCEDURES

General Methods

Standard molecular techniques including nucleic acid purification, restriction analysis, gel electrophoresis, DNA ligation, cloning, subcloning, dideoxy sequencing, probe radiolabeling, Northern and Southern analysis, RNase protection assays, and library screening were performed according to established protocols (22). Automated sequencing with Dyedeoxy primers or Dyedeoxyterminators was performed on an ABI 373 machine. Hybridizations of Northern, Southern, and zoo blot filters (Pall) were performed at 65 °C in 125 mM sodium phosphate (pH 7.2), 250 mM NaCl, 7% SDS, 10% polyethylene glycol. Filters were washed at 65 °C to final stringencies of 0.2 × SSC (1 × SSC: 150 mM NaCl, 15 mM sodium citrate, pH 7) for 10 min. Phage plaque hybridizations and subsequent washes were carried out under comparable stringency conditions.

Tissue Preparation and RNA Extraction

Preparations of E12.5 embryonic central nervous system samples were done as follows. Under a dissection microscope, brain tissue was separated from surrounding mesoderm and ectoderm. Neuroectodermal tissue spanning fourth ventricle through midbrain was separated from prosencephalic territories. Fresh tissue preparations from two CD1 litters were pooled and lysed in guanidine isothiocyanate. RNA extraction was carried out on a cesium chloride gradient (22).

RNA Fingerprinting

Clone 203 was derived through a modification1 of the RNA fingerprinting protocol (13) comparing mRNAs of mouse E12.5 mesencephalon, E12.5 prosencephalon, and postnatal mouse brain and cerebellum. RNA fingerprinting was conducted as follows. A reverse transcription reaction was carried out using a (dT)16 primer on total RNAs extracted by the cesium chloride method (22) and digested with 4 IU of DNase I/µg of total RNA. Radioactive PCR reactions were performed in duplicate from 1 µl of each RT reaction in a 50-µl final volume with an arbitrary 12-mer (DR34, sequence 5'-GACGAGGCTGGA) (final concentration, 4 µM). PCR conditions were 3 min at 94 °C, 2 min at 80 °C in which Taq polymerase was added (hot start), followed by 35 cycles of 1 min at 94 °C, 1 min at 50 °C, 30 s at 72 °C, with a final elongation step of 5 min at 72 °C. 0.1 µl of [alpha -32P]dCTP was added to each reaction. Amplified products were separated on a 5% denaturing acrylamide gel and visualized by autoradiography. Differentially displayed bands were cut from the gel and electroeluted. The bands were reamplified using the same 12-mer primers and blunt-end cloned into pBluescript II SK+ (Stratagene) as described (11).

Sequence Analysis

Data bank searches (GenBankTM, GenEmbl, SwissProt, and Protein Identification Resource) were run through the BLAST server (23). Additional sequence analysis and contig assembly was done using the MacVector program (Oxford Molecular Group) and the Sequencher program (Gene Code Corp.), respectively. The nucleotide sequence of the gene was deposited into the GenBankTM data base with accession number U71189.

Genetic Mapping

Genetic mapping was done on 96 DNAs corresponding to the parentals and 94 N2 progeny of a (C57BL/6j × SPRET/Ei)F1 × SPRET/Ei (BSS) backcross generated and distributed by The Jackson Laboratory (Bar Harbor, ME) (24). An MspI restriction fragment length polymorphism was identified in an intronic sequence and amplified with primers p1 and p2 (sequences 5'-GGTTGGCCATAGGAACATT and 5'-TCTTTCCAGCTCCCCAGC) as described under "Results." Its segregation was followed, and linkage analysis was performed with the MapManager 2.6 program (25).

Quantitative RT-PCR

Total RNAs from mouse preparations at E12.5, P4, and adult brain and liver were used in quantitative PCR reactions designed to work within the linear (exponential) range of amplification. Total RNA was reverse-transcribed using a random hexamer primer (Life Technologies, Inc.), and the quantity of cDNA synthesized in each RT reaction was first normalized by means of PCR amplifications with mouse glyceraldehyde-3-phosphate dehydrogenase primers (sequences 5'-CGCATCTTCTTGTGCAGTG and 5'-GTTCAGCTCTGGGATGAC). Each reaction was conducted incorporating 0.1 µl of [alpha -32P]dCTP in the mix. Amplification products were separated through polyacrylamide gels and quantitated by densitometry (Image Quant, Molecular Dynamics). At each stage examined, identical amounts of cDNA from each RT reaction were then used in two parallel PCR amplifications using Mmot1-specific primers (p1 and p2, see above). A touchdown PCR (26) was conducted using 3 min at 94 °C, 2 min at 80 °C in which Taq polymerase (Perkin-Elmer) was added; 2 cycles of 1 min at 94 °C, 1 min at 62 °C, 1 min at 72 °C; 2 cycles of 1 min at 94 °C, 1 min at 60 °C, 1 min at 72 °C; 2 cycles of 1 min at 94 °C, 1 min at 58 °C, 1 min at 72 °C; 21 cycles of 1 min at 94 °C, 1 min at 56 °C, 1 min at 72 °C; and a final elongation of 5 min at 72 °C.

In Situ Hybridization and Immunohistochemical Analysis of Mouse Tissue Sections

Radioactive and nonradioactive in situ hybridization (27) was carried out as follows. 7-µm paraffin serial sections from a single embryo were displaced in 4-6 adjacent series; two alternative series were used for each probe. Four embryos from at least two litters were studied at 12.5 and 13.5 days of embryonic development. Slides were deparaffinated in xylene, hydrated through an alcohol series, treated with paraformaldehyde and proteinase K, acetylated, and dehydrated through an ethanol series. For radioactive in situ studies, 1 µl (3 × 106 cpm) of Mmot1 riboprobe labeled with 35S-UTP (Amersham) in the hybridization mix was added to each slide. For nonradioactive in situ studies, 1 µg of Mmot1-linearized plasmid was transcribed in vitro in the presence of 0.8 µl of 10 mM digoxigenin-11-UTP (Boehringer Mannheim). Both sense and antisense probes were used. Hybridization was carried out overnight at 65 °C. Slides were washed under stringent conditions (65 °C, 2 × SSC, 50% formamide) and treated with RNase A. Autoradiography was performed with Kodak NT/B2 emulsion. Exposure times were 15 days. Sections were examined and photographed on dark and bright fields using a Zeiss SV11 microscope. Nonradioactive signal was revealed through an alkaline phosphatase-conjugated anti-digoxigenin antibody (Boehringer Mannheim) as recommended by the manufacturer. Immunohistochemical analysis was conducted with a monoclonal anti-PCNA antibody (clone PC10, Boehringer Mannheim). Sections were incubated with a peroxidase-conjugated secondary antibody, and signal was revealed with the Vectastain ABC kit (Vector Laboratories, Inc.) as recommended.

RNase Protection Assay

A Mmot1-specific 379-nt sequence was cloned into pBluescript and used to synthesize a riboprobe by in vitro transcription with T7 RNA polymerase and incorporation of [alpha -32P]UTP (800 Ci/mmol) (Amersham Life Science, Inc.). 50 µg of RNA from each of 11 different adult mouse tissues was hybridized, treated, and polyacrylamide gel electrophoresis-separated as described (28). Normalization was achieved through a mouse beta -actin riboprobe.

Gel Mobility Shift Assay

In vitro transcription, transcript purification, and translation using rabbit reticulocyte lysate (Promega) were done according to manufacturer recommendations, with the addition of 10 µM ZnSO4. The efficiency of in vitro translation was assayed by running parallel translation reactions performed in the presence of [35S]methionine (Amersham). The double-stranded synthetic DNA fragment carrying the binding site for the Ebf/Olf1 protein (5'-ACCCATGCTCTGGTCCCCAAGGAGCCTGTC) (29) and a control DNA fragment where the binding site had been mutated (5'-ACCCATGCTCTGGTCAGCAAGGAGCCTGTC) were end-labeled with [gamma -32P]ATP (Amersham) using 20 units of T4 polynucleotide kinase (Ambion Inc.) according to established protocols (28). In vitro DNA binding and electrophoretic mobility shift assays were performed as described (30), except that 2 µg of poly(dI-dC) were used as a nonspecific competitor. In each binding reaction (20 µl) we employed 0.15 pmol of labeled double-stranded DNA (about 40,000 cpm); 10 µl were then applied to a nondenaturing 6% polyacrylamide gel.


RESULTS

Cloning of Mmot1 by PCR-based Differential Screening

We applied a modified RNA fingerprinting protocol (11)1 to the analysis of differential gene expression in the embryonic and postnatal mouse brain. By RNA fingerprinting, we compared the following stages and districts: E12.5, mesencephalon + cerebellar primordium; E12.5, prosencephalon; P4, brainstem + cerebellum; P4, forebrain. As primers we employed a panel of arbitrary 12-mers, some of which were carrying a partially degenerate position at their 3' ends obtained through computer simulations of PCR experiments run on a nonredundant mouse nucleotide data base.1 At embryonic day 12.5, band 203 (778 nt, nucleotides 939-1717 of the full-length transcript), obtained with primer DR34, amplified almost exclusively in the posterior region (metencephalon-mesencephalon), whereas it failed to show an obvious band at postnatal day 4 (P4) either in the anterior or posterior head (Fig. 1). The band was gel-excised, reamplified, and cloned into pBluescript II SK- (Stratagene). Clones were screened as described (11), and plasmid 203.14 was manually sequenced. A data base search was run with BLASTN and BLASTX using the Genetics Computer Group interface (31). The search revealed 72% identity at the nt level with a mouse gene named Ebf for early B-cell factor (18) encoding a helix-loop-helix transcription factor. Ebf is virtually identical to an independently cloned rat gene named Olf1 (olfactory transcription factor 1) (19). To confirm that Mmot1 and Ebf are indeed different genes, we analyzed the Mmot1 cDNA by restriction mapping, identifying SacII and HinfI restriction sites absent from Ebf (or Olf1) (positions 1529 and 1472 of the Mmot1 transcript, respectively) as predicted from sequence analysis.


Fig. 1. RNA fingerprinting experiment comparing cDNAs from anterior and posterior head territories on the 13th day of prenatal development (E12.5) and on the 5th day of postnatal development (P4). RNA fingerprinting RT-PCR reactions were conducted in duplicate at each stage examined. Arrowhead, band 203 corresponding to the Mmot1 transcript.
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Genetic Linkage Analysis Defines Mmot1 as a New Member of the Ebf/Olf1-like Gene Family

Genetic evidence obtained by other authors (32) had assigned Ebf to proximal mouse chromosome 11. To strengthen our evidence defining Mmot1 and Ebf as distinct genes, we set out to localize Mmot1 in the mouse genome by linkage analysis in the BSS backcross generated and maintained at The Jackson Laboratory (24). Using a primer pair (p1 and p2) from a region of low degree homology with Ebf, we amplified a 2.7-kilobase genomic fragment spanning an intronic sequence in the coding portion of the gene. The experiment was conducted on the parental strain DNAs of the BSS backcross (C57BL/6JEi, B6, and SPRET/Ei spretus). Automated sequencing of the product ends confirmed them as part of the Mmot1 gene. The PCR product was digested with frequent cutters (RsaI, Sau3AI, TaqI, and MspI). An MspI polymorphism was identified consisting of 2,450- and 250-base pair fragments in B6 DNA and a 2,700-base pair fragment in spretus DNA. This polymorphism was employed to type the 94 individual N2 progeny of the BSS backcross by PCR and restriction fragment length polymorphism analysis. 93 out of 94 progeny were typed successfully. Linkage analysis performed with MapManager 2.6 unequivocally localized Mmot1 to mouse chromosome 14, 1.1 centimorgan distal to Raftk (33) (lod score 25.6) and 2.3 centimorgan proximal to Nfl3 (34) (lod score 22.6). The data are summarized in Fig. 2. The human homolog of Nfl has been mapped to chromosome 8p21 (35, 36).


Fig. 2. Mapping of Mmot1 in the mouse genome. Above, haplotype and linkage analysis of Mmot1 and flanking loci on mouse chromosome 14 through the analysis of the BSS backcross (The Jackson Laboratory). Empty squares indicate the Mus spretus allele; solid squares indicate the C57BL/6J allele. Shaded squares, genotype not determined. Numbers to the right indicate recombination fractions ± S.E. and lod scores. Columns represent different haplotypes observed on chromosome 14. Numbers below columns define the number of individuals sharing each haplotype. Below, position of Mmot1 on chromosome 14 with respect to nearby markers independently mapped by others on the BSS backcross. Numbers on the left represent approximate genetic distances from the most centromeric chromosome 14 marker in this cross.
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Cloning of the Full Coding Sequence

Based on the above evidence, we set out to isolate clones spanning the entire coding sequence of Mmot1. To this end, we plated out 6 × 105 plaque-forming units from an embryonic day 11.5 whole-embryo cDNA library (CLONTECH, ML1027). Again, as a probe, we utilized a region of Mmot1 displaying low degree similarity to Ebf. After high stringency hybridization and washes, we isolated five positives, one of which spanned the full-length transcript (5.4 kilobases, corresponding to the band detected by Northern analysis and not shown). By adopting a strategy involving both shotgun cloning and primer walking, we obtained the double strand sequence of the cDNA. The sequence contains a 1659-base pair open reading frame preceded by an in-frame stop codon (TAA, -66).

Protein Sequence Analysis

The deduced peptide sequence (553 residues) was analyzed with a variety of local and on-line programs. The primary sequence is 80.6% identical to the Ebf protein, with more conserved regions clustered around a putative zinc finger domain (residues 134-147, sequence HEVMCSRCCEKKSC) and a helix-loop-helix domain (residues 344-387, sequence KEMLL ... VPRNP). Also perfectly conserved is a putative nuclear targeting domain (residues 219-223, sequence RRARR). A Drosophila melanogaster gene named collier (accession number X97803) was also found to encode a highly similar protein (20). The deduced peptide sequence of Mmot1 is illustrated in Fig. 3 and compared with the two other known members of the subfamily (Ebf/Olf1 and Collier).


Fig. 3. Sequence alignment of Ebf/Olf1, Mmot1, and the Drosophila melanogaster protein Collier. Boxed residues are identical in different proteins, from N terminus to C terminus. Shaded residues represent the zinc finger element, nuclear targeting domain, and helix-loop-helix domain, respectively. The second helix of the conserved HLH domain is not present in the Collier protein.
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Mmot1 Belongs to an Expanding, Phylogenetically Conserved Gene Family

Conservation of the Mmot1 gene was assessed experimentally by zoo blot analysis. A Southern blot containing DNAs of six mammalians, a frog, and chicken was hybridized with a fragment of the Mmot1 coding sequence (positions 1340-2340) that shows the highest degree of divergence from Ebf. Both hybridization and washes were carried out at high stringency conditions (see "Experimental Procedures"). The experiment suggested the existence of strongly conserved homologs of Mmot1 in all organisms tested, including chicken and Xenopus laevis (Fig. 4a).


Fig. 4. Phylogenetic conservation. a, high stringency Southern (zoo blot) analysis of 10 µg of DNA from each of eight mammalian and nonmammalian species. The filter was hybridized with an Mmot1-specific probe displaying >40% divergence from the corresponding region of the Ebf transcript. For details on hybridization and washing conditions, see "Experimental Procedures." b, the analysis of the EST data base revealed the existence of several possible new members of the Ebf family of HLH factors in the human and mouse genomes. W14732 is an adult mouse brain EST, clearly distinct from Ebf and Mmot1. Boxed nucleotides are identical in at least two sequences. The region shown corresponds to nt 956-1305 of the Ebf gene and 1038-1387 of the Mmot1 gene, spanning the segment encoding the HLH domain.
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Because the isolation of Mmot1 in mouse defines a new family of closely related mammalian HLH proteins, we looked to identify possible new homologs of our gene and Ebf/Olf1 in the Expressed Sequence Tag (EST) data base (37). The search was conducted with the BLAST programs using the Mmot1 protein as a query sequence and revealed the existence of at least one other member of the same family, represented in the murine (W14732) (Fig. 4b) and human (W21838) EST collections. Murine EST W14732 and human EST W21838 are probably orthologous to each other and distinct from Ebf/Olf1 and Mmot1. At the nucleotide level, Mmot1 and W14732 are 77.1% identical. As mentioned, a search of GenBankTM revealed a homolog of Ebf/Olf1 and Mmot1 in Drosophila melanogaster (collier, accession number X97803). At the nucleotide level, Mmot1 and collier are 70.9% identical. One additional member of the same gene family was identified in Caenorhabditis elegans (accession number C13312). Finally, a dedicated analysis of the complete yeast genome through the TBLASTN program identified no high score homologs in that unicellular eukaryote.

Expression of Mmot1 in the Midgestation Embryo and Adult

To confirm the expression data obtained by RNA fingerprinting (Fig. 1) and quantitative RT-PCR (not shown) and to finely characterize the distribution of our transcript in the embryo, we performed in situ hybridization of mouse tissue sections at embryonic days 10.5-14.5 (Fig. 5). As a riboprobe, we employed a 377-nt fragment (position 1340-1717) displaying 66.3% identity to Ebf.


Fig. 5.

mRNA in situ hybridization of embryonic tissue sections at 10.5 (a and b), 12.5 (c-g), 13.5 (h-n and s), and 14.5 (o-q) days postcoitus. b, f, l, m, n, q, and s are coronal sections; all others are sagittal sections. g, k, and n are negative controls, hybridized with a sense riboprobe. r, schematic summary of transcript distribution at E12.5-13.5. s, nonradioactive in situ hybridization of an E13.5 mesencephalic coronal section. t, histochemical analysis (anti-PCNA monoclonal antibody) of an adjacent section. Cb, cerebellum; drg, dorsal root ganglia; fp, floor plate; DT, dorsal thalamus; GE, ganglionic eminence; I, isthmus; M, mesencephalon; ne, nasal epithelium; p1-p4, prosomeres; r1-r4, rhombomeres; rp, roof plate; sc, spinal cord; T, telencephalon; V, trigeminal ganglion; or, optic recess.


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E10.5

Mmot1 expression (Fig. 5, a and b) is localized to the whole rhombencephalon (r1-r4) and spinal cord caudally and to the mesencephalon (M) and pretectal anlage (prosomere 1) rostrally. Both alar and basal plate cells are positive, unlike the floor plate (fp) and roof plate (rp). In the peripheral nervous system, the gene is expressed in the trigeminal ganglion (maxillary mesenchyme) and in the dorsal root ganglia. Mmot1 expression was also detected in the dorsal maxillary epithelium.

E12.5

At this stage (Fig. 5, c-f, negative control in g), the expression of Mmot1 includes a rostral domain (metencephalic-mesencephalic territory, excluding the basal portion of the fossa isthmica), a caudal domain (spinal cord), and the anlage of the nasal epithelium. Rostrally, expression stops between mesencephalon and p1, to resume weakly in prosomere 1 and, more strongly, between p1 and the dorsal thalamus (p2), caudal to the retroflex or habenula-interpeduncular tract; the epithalamus is clearly negative. Moreover, low level signal is observed in the preoptic area and mamillary region. In the r1-r4 interval, expression spans the cerebellar plate and pontine nuclei. Caudally, the expression domain stops abruptly at the r4-r5 interrhombomeric boundary, to resume from r7 all the way to the tail, and is restricted to the alar plate neuroepithelium. The fossa isthmus does not present labeling in its ventral midline, whereas the trigeminal and dorsal root ganglia are still positive.

E13.5-14.5

At these stages (E13.5: Fig. 5, h-n, negative controls in k and n; E14.5: Fig. 5, o-q) central nervous system areas expressing the gene at high levels are virtually restricted to the mesencephalon and metencephalon, whereas the cerebellar plate appears less intense in its ventricular zone. Very weak labeling is also observed in the alar plate of the rostral diencephalon and telencephalon. While the alar spinal cord expresses Mmot1 at E13.5, this expression disappears at E14.5. The distribution of Mmot1 in the context of the prosomeric model (3) is schematically summarized in Fig. 5r. Extraneural labeling for Mmot1 can be detected in the limb bud. At all stages examined, very strong signal is present in the olfactory portion of the nasal cavity, with a sharp demarcation at the boundary with the respiratory epithelium, which does not express the gene. No other major expression sites could be seen at the embryonic stages examined.

High resolution, nonradioactive in situ analysis was conducted on E13.5 sections with an Mmot1 cRNA probe (Fig. 5s). In parallel, immunohistochemical analysis was carried out on adjacent sections with an anti-PCNA monoclonal antibody (Fig. 5t) used as a marker of proliferating neuroblasts (38). The comparison reveals that Mmot1 is expressed in a thin postmitotic stratum of the ventricular wall, apical to the interkinetic migration range of neuroepithelial cells.

To extend the characterization of Mmot1 expression beyond embryonic development, we determined the distribution of the transcript in 11 adult mouse tissues by RNase protection assay (Fig. 6). In the adult mouse, Mmot1 is specifically expressed in the cerebellum, muscle, heart, ovary, and testis. Lower expression levels are shown in the adult brain. Very low level or absent signal is observed in the thymus, kidney, liver, spleen, and intestine.


Fig. 6. Expression of Mmot1 in 11 adult mouse tissues tested by RNase protection assay with a 379-nt Mmot1-specific antisense riboprobe featuring 36% divergence from the corresponding region of the Ebf cDNA. Persistent expression in the adult cerebellum and, at very low levels, in the adult brain is revealed by this assay.
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Mmot1 Binds the Nucleotide Target Site of Olf1 in Vitro

To characterize the DNA binding properties of Mmot1, in a comparison with its close cognate Ebf/Olf1, we synthesized the protein by in vitro transcription and translation of a full coding Mmot1 cDNA subclone (ab1). The translation product (60 kDa) matched the size of our deduced amino acid sequence. The DNA binding domains of Mmot1 and Ebf/Olf1 are extremely similar, and we therefore tested whether Mmot1 could recognize the same sequence bound by Ebf/Olf1. Labeled double-stranded oligonucleotides corresponding to the wild type and mutant Olf1 recognition sites (29) were employed in a gel mobility shift assay. A band shift was observed after incubation of the Mmot1 protein produced by in vitro translation with the wild type double-stranded oligonucleotide but not with a mutant double-stranded oligonucleotide in which the Olf1 target site was disrupted (Fig. 7).


Fig. 7. Gel mobility shift assay revealing specific affinity of the Mmot1 protein for the Olf1 nucleotide binding site described in Ref. 29. Lanes contain the following: no lysate, gamma -32P end-labeled double-stranded oligonucleotide; RRL, same as no lysate but with the addition of rabbit reticulocyte lysate; 4 µl and 1 µl, in vitro synthesized, unlabeled protein after incubation with 4 and 1 µl gamma -32P end-labeled double-stranded oligonucleotide, respectively. A band shift (doublet) is observed after incubation of Mmot1 with the wild type (wt) nucleotide site but not with a mutant (mut) nucleotide site featuring two substitutions that disrupt the palindrome (see "Experimental Procedures" for sequences of wild type and mutant sites).
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DISCUSSION

The present paper reports the successful application of a PCR-based, internally primed RNA fingerprinting technique (13, 39)1 to the isolation of a gene displaying a restricted pattern of expression in the midgestation embryonic mouse brain. Among other potentially significant results,4 this approach has led to the identification of Mmot1, a new helix-loop-helix-type DNA-binding protein homologous to Ebf/Olf1 (19). To date, this family included three known members: a rat gene named Olf1 (19), a virtually identical mouse gene named Ebf (32), and the Drosophila gene collier (20).

collier appears to be involved in anterior head patterning in Drosophila. Its expression is dependent on a gene expression program involving cytoplasmic polarity genes (bicoid) and gap rule genes (buttonhead, in particular) (20). A phenocopy of the collier mutant, produced by transgenic insertion of an antisense construct, has displayed down-regulation of the En gene in the intercalary segment but not in the mandibular one (20). In Drosophila, collier is only expressed in actively proliferating territories of neuroectodermal origin (20). In a comparison with Mmot1 and Ebf/Olf1, the Drosophila protein appears to lack the second helix of the HLH domain featured in its mammalian counterparts (Fig. 3). This suggests that Collier might be unable to assemble into dimers or bind a palindromic sequence and thus might be involved in the transcriptional regulation of a nonoverlapping developmental pathway with respect to its mammalian homologs.

Although the mouse gene Ebf and the rat gene Olf1 are reportedly translated from different start sites, they most likely represent orthologous sequences and encode virtually identical proteins (18, 19), with well characterized regulatory functions in B-cell and olfactory development. In the present paper, we provide genetic evidence that Mmot1 represents a separate, phylogenetically conserved member of the same expanding gene family. Moreover, through the analysis of the EST data base (37, 40), we report the identification of one high quality homologous sequence (accession number W14732) corresponding to an adult mouse brain cDNA, suggesting the existence of an additional, as yet uncharacterized, Ebf/Olf1-like transcription factor gene in mammalian genomes.

At all stages tested by in situ hybridization, the Mmot1 transcript is found in a finely delimited marginal stratum of the neural tube. The distribution of Mmot1 compared with that of a proliferation marker (PCNA) (38) suggests that our gene is expressed precociously in a nascent postmitotic, subventricular layer, possibly involved in the genesis and/or differentiation of specific neural cell types.

The Mmot1 transcript is expressed from the mesencephalon to the tail at 10.5 days of embryonic development but becomes restricted to the metencephalon-mesencephalon and nasal neuroepithelium at E14.5, after which it remains expressed in the adult cerebellum. Between E12.5 and E13.5, the embryonic distribution of the Mmot1 transcript presents the uncommon feature of a sharp anterior expression boundary within the embryonic diencephalon (between prosomere 1 and 2), with low level expression in prosomeres 3-5 and undetectable levels in the telencephalon. This property may contribute to the definition of factors involved in the fine subdivision of the embryonic forebrain, providing a posterior boundary (between p1 and p2) in the context of the prosomeric model reviewed in Refs. 3 and 41.

In addition to displaying high expression levels in the cerebellar primordium, midbrain, and dorsal thalamus, Mmot1 is transcribed at remarkable levels in the olfactory neurons of the nasal cavity and vomeronasal organ. Olf1, a closely related gene, was found by other authors to drive the expression of several olfactory-specific proteins (19). However, mice homozygous for induced mutations of Ebf, the mouse ortholog of Olf1, display no alterations in the morphogenesis of the olfactory area or in the expression of olfactory proteins (42). Likewise, they present with no obvious abnormalities in midbrain or hindbrain development. Evidence of strong similarities in the expression of Ebf/Olf1 (42) and Mmot1 (present paper) at many sites in the developing brain and nasal neuroepithelium provides a possible explanation for the lack of neurodevelopmental and olfactory defects in Ebf-/- knockout mutant mice. The notion of genetic redundance in the pathway involving Ebf/Olf1 and Mmot1 is strengthened by in vitro functional evidence presented in this paper, which proves that Ebf/Olf1 and Mmot1 share DNA binding affinity and specificity (29) as expected based on the marked similarities in their dimerization and DNA binding domains. Moreover, similarities in the HLH domains of the two proteins suggest the possibility that they might assemble as heterodimers in those territories where the corresponding genes are coexpressed. In this scenario, the generation of Mmot1 lack- or gain-of-function mutants and the analysis of double knockout mutants for Mmot1 and Ebf will assist in the genetic dissection of functional pathways involving the two genes while clarifying their role in midbrain/hindbrain subdivision and olfactory development.

In summary, Mmot1 is a new, developmentally regulated, restrictedly expressed member of a novel, expanding family of neural transcription factors whose analysis may have considerable impact on the study of midgestation neural development in general and the mechanisms of normal and aberrant cerebellar ontogeny in particular.


FOOTNOTES

*   This work was supported by Italian Telethon Grant B14 and European Union Grant BMH4-CT96-0777.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) U71189.


§   These two authors contributed equally to this work.
**   To whom correspondence should be addressed: DIBIT-HSR, via Olgettina 58, I-20132 Milano, Italy. Fax: 39-2-26434855; E-mail: consaleg{at}dibit.hsr.it.
1   G. G. Consalez, A. Cabibbo, A. Corradi, C. Alli, M. Sardella, R. Sitia, and R. Fesce, submitted for publication.
2   The abbreviations used are: PCR, polymerase chain reaction; HLH, helix-loop-helix; RT, reverse transcription; PCNA, proliferating cell nuclear antigen; contig, group of overlapping clones; BSS, (C57BL/6j × SPRET/Ei)F1 × SPRET/Ei; nt, nucleotide(s); EST, expressed sequence tag.
3   R. Turner and J. Nadeau, unpublished data.
4   A. Corradi and G. G. Consalez, unpublished data.

ACKNOWLEDGEMENTS

The contribution of Alessandro Bulfone (Telethon Institute of Genetics and Medicine, Milan) has been truly essential for completion of this work. Thanks to Antonello Mallamaci for helpful discussions and to Fabio Grassi for the gift of mouse RNAs. We thank Lucy Rowe and Mary Barter (The Jackson Laboratory) for sharing the BSS genetic mapping panel. We are deeply thankful to the late Emilia Viganotti for her generous donation to our study of brain development.


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