(Received for publication, November 5, 1996, and in revised form, June 18, 1997)
From the UMR 5539 CNRS, Département Biologie-Santé, Université Montpellier II, France and § Department of Biological Sciences, University of Warwick, Coventry CV4 7 AL, United Kingdom
Ricin is a heterodimeric protein toxin. The ricin A chain is able to cross the membrane of intracellular compartments to reach the cytosol where it catalytically inactivates protein synthesis. It is linked via a disulfide bond to the B chain, a galactose-specific lectin, which allows ricin binding at the cell surface and endocytosis. To examine the potential of ricin A to carry proteins into the cytosol and the requirement for unfolding of the passenger protein, we connected mouse dihydrofolate reductase (DHFR) to ricin A by gene fusion via a spacer peptide. DHFR-ricin A expressed in Escherichia coli displayed the biological activities of the parent proteins and associated quantitatively with ricin B to form DHFR-ricin. The resulting toxin was highly cytotoxic to cells (4-8-fold less than recombinant ricin). DHFR-ricin cytotoxicity was inhibited by methotrexate, a DHFR inhibitor stabilizing DHFR-ricin A in a folded conformation. The DHFR moiety of DHFR ricin bound to the plasma membrane. Although methotrexate prevented this binding, it did not significantly affect DHFR-ricin endocytosis, which proceeded via ricin B chain. Intoxication kinetics data and a cell-free translocation assay demonstrated that protection of cells from DHFR-ricin cytotoxicity resulted from a selective inhibition by methotrexate of DHFR-ricin A translocation. We conclude that ricin A is a potential carrier of proteins to the cytosol, provided that the passenger protein is able to unfold for transmembrane transport.
Toxins such as ricin and diphtheria toxin (DT),1 which have intracellular targets, are able to cross particular biological membranes. Both ricin and DT are heterodimeric proteins comprising an A chain bearing the catalytic activity responsible for the arrest of protein synthesis and a B chain enabling the toxin to bind at the plasma membrane (1, 2). Whereas DT recognizes a specific receptor (3), the lectin activity of ricin B chain (RTB) enables interaction with terminal galactose residues present on both glycoproteins and glycolipids. Ricin is then endocytosed, and electron microscopic studies revealed significant routing to endosomes and to the trans-Golgi network (2). Expression of mutant GTPases involved in regulating vesicular transport steps early in the secretory pathway protects cells against ricin toxicity, suggesting that this toxin may be further transported to the endoplasmic reticulum (4). At some point during ricin intracellular routing, the A chain is translocated across a membrane into the cytosol where protein synthesis is inhibited. This translocation step is rate-limiting for cytotoxicity (5), and a number of potential translocation sites are reported in the literature, namely the endosome (6), the trans-Golgi network/Golgi apparatus (7), or the endoplasmic reticulum (4). Nevertheless, a direct assay for ricin translocation is available only in the endosome system (6).
The unfolding requirement for protein translocation has essentially been studied using mouse dihydrofolate reductase (DHFR), which once fused to the appropriate targeting sequence, could be transported across the inner membrane of Escherichia coli (8), imported into mitochondria (9), or into the ER lumen (10). Such transport processes are blocked by methotrexate (MTX), a folate analogue that stabilizes the native conformation of DHFR, thereby preventing the unfolding deemed necessary for the membrane translocation of most polypeptides (8-10). However, when DHFR was targeted to the chloroplast (11), or the glycosome (12), import was not affected by MTX or its analogue, aminopterin. In the former case at least, this is due to a potent unfolding activity associated with the chloroplast surface (13).
Mutant DT A chain (DTA) and ricin A chain (RTA) with introduced disulfides were either not or poorly translocated (14, 15), indicating that toxin A chains must also unfold to cross membranes. Bacterial toxins such as Pseudomonas exotoxin A (16) and DTA (17, 18) can transport foreign proteins into the cytosol. This transfer also requires unfolding of the passenger protein as shown for DTA with both acidic fibroblast growth factor (17) and DHFR (18), using heparin and MTX, respectively, to stabilize the folded structure of the passenger protein and arrest translocation.
Nothing is known concerning the potential of RTA to deliver foreign proteins to the cytosol. Ultimately, however, enzymatically inactive ricin, like DT, is a potential carrier of antigenic peptides (19). This is of special interest since an initial step in the presentation of antigens by the major histocompatibility complex class I molecules requires peptide processing in the cytosol of antigen-presenting cells (20). Ricin may thus be used for cytosolic delivery of such peptides or even of whole proteins for subsequent processing, cell surface presentation and induction of a protective cytotoxic T lymphocyte (CTL) response (16, 19).
In this study, we genetically fused DHFR to RTA and demonstrated the ability of RTA to introduce fused DHFR into the cytosol. This import was blocked by MTX, indicating that DHFR must unfold in order for it to translocate with RTA. This finding has obvious implications for the use of RTA as a carrier of cellular epitopes.
All enzymes needed for DNA manipulations were obtained from Life Technologies, Inc. or Pharmacia. Taq DNA polymerase for polymerase chain reaction, plasmid miniprep purification system, DNA silver sequencing kit, and reticulocyte lysates were from Promega. Most of the chemicals were obtained from Sigma. Pure RTB (without any detectable RTA) was purchased from Inland Biologicals, Austin, TX, whereas recombinant RTA (rRTA) was provided by Zeneca (UK). Western blotting detection kit and radiochemicals were from Amersham.
Preparation of the DHFR-RTA ChimeraAn
EcoRI-PstI DHFR fragment was obtained by
polymerase chain reaction using the full-length coding sequence of the
mouse DHFR in pDS5/3 vector (21) as a template. The sense primer
(CTAAGAATTCATGGTTCGACCATTG) was used to introduce an
EcoRI site (
) immediately upstream of the initiation
codon. The antisense primer (CTTACTGCA
GGTCTTTCTTCTCGTA) provided a
PstI cleavage site (
) immediately before the natural stop
codon. The full-length RTA-coding sequence (22) in pKK 223.3 (Pharmacia) was used as a template to prepare by polymerase chain
reaction an PstI-HindIII RTA fragment. The sense
primer (GATCTCTGCA
GATATTCCCCAAACAA) provided a PstI site
(
) before the second codon of RTA and the antisense primer
(TTACCA
AGCTTTCAAAACTGTGACGA) provided a HindIII
site (
) after the stop codon. Polymerase chain reaction products
were gel-isolated after restriction and ligated in a stepwise manner
into pKK 223.3. Preliminary experiments showed that the fusion protein
prepared without any spacer was inactive in several assays for
biological or biochemical activities (data not shown). A
double-stranded oligonucleotide containing an internal BamHI
site and coding for a spacer peptide
(His-Ala-Ser-Thr-Pro-Glu-Pro-Asp-Pro-Val) was thus inserted using the
PstI site. This peptide linker is similar to the flexible
hinge region of a monoclonal antibody (23). Purified plasmids were
restricted with PstI before transformation of E. coli TG2. Clones were screened for acquisition of the
BamHI restriction site, and DNA sequencing enabled the
assessment of its orientation. The resulting protein is denoted
DHFR-RTA.
A
1.5-liter culture of E. coli TG2 containing the DHFR-RTA
plasmid was grown at 30 °C. Expression was induced at an
A595 ~ 0.4 using 1 mM
isopropylthiogalactoside. After 2 h at 30 °C, E. coli lysates were prepared by sonication, clarified by
centrifugation for 30 min at 20,000 × g (22), and
dialyzed against 20 mM potassium phosphate, pH 7.4, 1 mM EDTA, 0.1 mM phenylmethylsulfonyl fluoride, 0.5 mM dithiothreitol (buffer A) before loading (at 0.2 ml/min) onto a 5-ml MTX-agarose column (Sigma). After washing with 45 ml of buffer A and 60 ml of buffer A supplemented with 1 M
KCl, DHFR-RTA was eluted with 1 mM MTX in buffer A and
stored sterile at 80 °C, after adding 15% glycerol. The final
yield of purified protein was greater than 10 mg/liter of culture. DHFR
activity was measured as described previously (24). The ability of RTA to inhibit protein synthesis was assayed using rabbit reticulocyte lysates (25) supplemented with globin mRNA (Life Technologies, Inc.).
The two proteins (20 µM each) were mixed in PBS in the presence of 8 mM GSH. After 3 h at room temperature and overnight dialysis at 4 °C against PBS, the mixture was analyzed by nonreducing SDS-PAGE. Recombinant ricin (rRTA-RTB) was prepared using the same protocol. We experienced that 125I labeling of isolated RTA impaired subsequent association with RTB (data not shown). DHFR-RTA, as well as rRTA, used as a control throughout this study, was thus radiolabeled with 125I (26) after association with RTB. Transferrin and DHFR-ricin were conjugated with FITC, and ricin was complexed to colloidal gold as described elsewhere (27).
Endocytosis and Cell-free Translocation AssaysEndocytosis efficiency of radiolabeled DHFR-RTA-RTB (DHFR-ricin) by mouse BW5147 lymphocytes was measured using recombinant ricin as a control. Washes with 0.1 M lactose were used to displace plasma membrane-bound molecules (6, 28).
The assay for toxin translocation from purified lymphocyte endosomes is
described elsewhere (6). 125I-Transferrin, as a
membrane-bound tracer, and 125I-horseradish peroxidase, as
a soluble maker, were used as negative controls in all translocation
experiments to monitor the integrity of endosomes. BW5147 cells were
labeled with DHFR-ricin for 30 min at 37 °C in Dulbecco's modified
Eagle's medium containing 0.2 mg/ml bovine serum albumin and 0.15 mg/ml low density lipoproteins/ml, before lactose-scraping to displace
membrane-bound ligand, ricin-gold binding, and lysis under hypotonic
conditions. Unbroken cells and nuclei were removed by low speed
centrifugation, and crude membranes collected by ultracentrifugation.
They were then layered on a discontinuous sucrose gradient
(40%/30%/20% sucrose). After 2 h at 100,000 × g, endosomes were obtained from the 30%/20% interface, washed, and finally resuspended in translocation buffer (110 mM KCl, 15 mM MgCl2, 1 mM dithiothreitol, 0.15 mg/ml bovine serum albumin, 20 mM Pipes, pH 7.1, supplemented with ATP except when otherwise indicated). Translocation was assayed for 2 h at
37 °C and was stopped by chilling on ice. The medium was then
separated from endosomes by ultracentrifugation (160,000 × g for 5 min) on a 17% sucrose cushion. Translocated
proteins were precipitated with 10% trichloroacetic acid and separated
by reducing SDS-PAGE before autoradiography. Quantification was
performed by densitometric analysis of films exposed within their
linear range of detection. Direct counting of sliced gels was
occasionally used to follow 125I-rRTA or
125I-RTB translocation and gave identical results. When
translocation of unlabeled DHFR-ricin and recombinant ricin was
examined, Western blots (29) of translocated proteins were quantified
using a Storm apparatus (Molecular Dynamics).
Exponentially growing BW5147 cells were washed, then labeled with DHFR-ricin-FITC and ricin-tetramethylrhodamine isothiocyanate for 30 min at 37 °C in Dulbecco's modified Eagle's medium/bovine serum albumin/low density lipoprotein (see above). After lactose scraping, cells were fixed for 15 min at 2 °C in PBS containing 3.7% paraformaldehyde before quenching for 15 min using 50 mM NH4Cl in PBS. They were then washed with PBS, mounted in PBS supplemented with 2.5% 1,4-diacylbicyclo(2,2,2)octane, and examined under a Leica confocal microscope using a 63× lens and medial optical sections. Bleed through from one channel to the other was negligible. In preliminary experiments transferrin-FITC and an anti-mouse CD45 (clone I3/2) labeled with Cy5 were used as endosomal and plasma membrane markers, respectively (27).
Cytotoxicity AssaysCells (18,000 BW5147 in RPMI, 10% fetal calf serum or 6,000 L929 in RPMI, 5% fetal calf serum) were seeded in 96-well plates. Toxin solutions were added immediately (BW5147) or after 2 h at 37 °C to allow cell adherence (L929). After 24 h at 37 °C [35S]methionine (0.25 µCi) was added, and cells were incubated for a further 12-18 h. Precipitation with trichloroacetic acid was then performed either directly (BW5147) or following solubilization with 0.1 N NaOH after aspiration of the medium (L929). Proteins were collected onto glass fiber filters, washed with 5% trichloroacetic acid, then dried for radioactivity determination. Background incorporation was obtained from cells treated with 1 mM cycloheximide.
To examine the kinetics of protein synthesis inactivation by recombinant ricin and DHFR-ricin the above protocol was modified. The cell number was increased to 70,000/well (BW5147) or 15,000/well (L929). A pulse incorporation of [35S]methionine (1 h) was performed at various times after the start of intoxication.
Study of DHFR-RTA Stability during Intoxication by DHFR-RicinRadiolabeled toxin (5 nM of 125I-DHFR-ricin or 125I-recombinant ricin) was added to BW5147 cells (106/3 ml of RPMI/fetal calf serum) in the presence or absence of 50 nM MTX. After 0-24 h at 37 °C, cells were collected by centrifugation, washed three times with PBS, then lysed in 1 ml of immunoprecipitation buffer (20 mM Tris, 150 mM NaCl, 1 mM EDTA, 1 mM phenylmethylsulfonyl fluoride, 1% Nonidet P-40) (30). After 20 min on ice, insoluble material was removed by centrifugation (13,000 × g for 10 min). This step and the rest of the experiment were performed at 4 °C. The cleared lysate received 2 µl of sheep anti-RTA antibody and was mixed for 1 h on a rotating wheel before adding 7 µl of protein G-agarose (Sigma). After 1 h on the wheel, the immune complexes were recovered by centrifugation (10,000 × g for 3 min), washed twice with immunoprecipitation buffer, then once with PBS, and eluted by boiling in SDS-PAGE reducing sample buffer (30). Gels were exposed to storage phosphor screens that were analyzed using a Storm apparatus.
All experiments were done at least in triplicate and repeated twice. Errors are expressed as S.E.
Both DHFR (18) and
RTA (31) have been fused directly with a targeting protein to prepare
biologically active hybrids. To obtain a biologically active DHFR-RTA
chimera, we found it necessary to insert a linker peptide including
three prolines (23) between the N terminus of recombinant RTA and the C
terminus of DHFR (data not shown). The fusion protein was expressed in E. coli. A major band corresponding to the expected
molecular mass of 50 kDa and reacting to both anti-RTA and anti-DHFR
sera was observed on the gel of proteins from bacteria where expression was induced (Fig. 1).
The DHFR-RTA fusion was purified to homogeneity by affinity
chromatography using immobilized MTX (MTX-agarose), as ascertained by
protein staining and Western blotting using antisera against both DHFR
and RTA (Fig. 2). In particular,
cross-reactive material observed in E. coli sonicates using
anti-DHFR sera was eliminated by this single chromatographic step
(compare lane 4 in Fig. 1 and lane 3 in Fig. 2).
A typical yield was greater than 10 mg/liter of culture.
Since DHFR-RTA was purified using its affinity for MTX, it is clear that the DHFR portion of the chimera recognizes MTX. This binding is known to stabilize the folded conformation of DHFR (9). The high protease resistance of DHFR-RTA, probably due to the well documented resistance of the RTA moiety of the chimera to proteases (13), prevented us from monitoring the folding of the DHFR moiety using protease protection experiments, as conventionally performed (8-10, 18). On the other hand, this resistance conferred intracellular stability on the fusion protein (see below).
After purification, DHFR-RTA was tested on rabbit reticulocyte lysates for its ability to inactivate protein synthesis. The resulting IC50 (30 ± 10 nM) is similar to that of rRTA (15 ± 7 nM). DHFR-RTA also exhibited a DHFR activity (2.5 108 units/mol), which is virtually identical to the catalytic activity of native mouse DHFR (2.7-2.8 108 units/mol) (11). Together these data indicate that connection of DHFR to the N terminus of rRTA did not affect enzymatic activity of either of the parent proteins.
Preparation of DHFR-RicinMixing equimolecular amounts of the
DHFR-RTA with RTB and 8 mM GSH before dialysis generated
homogenous DHFR-RTA-RTB (DHFR-ricin) as seen by nonreducing SDS-PAGE
(Fig. 3, lane 6). This shows
that the chimera, DHFR-RTA, interacts as efficiently as rRTA with the B
chain. Formation of RTB dimers is observed only when the A chain is
absent from the mixture (lane 4). Recombinant ricin
(rRTA-RTB, lane 1) was generated using the same procedure.
This toxin was used as a control throughout the rest of this study.
Recombinant ricin migrated slightly faster (lane 1) than
native ricin (shown in lane 2) due to the absence of
oligosacharides on the A chain.
DHFR-Ricin Is Highly Cytotoxic
To examine the ability of rRTA to bring DHFR in the cytosol we first investigated intoxication of various cell types by DHFR-ricin. The results of these tests are summarized in Table I. The isolated chains of ricin were essentially nontoxic, whereas DHFR-RTA surprisingly showed moderate cytotoxicity, indicating that fused DHFR somehow promotes more efficient RTA uptake. Nevertheless, since association of DHFR-RTA with RTB to produce DHFR-ricin increased toxicity by 150-650-fold (Table I), DHFR-mediated internalization was not significantly involved in DHFR-ricin cytotoxicity as compared with uptake via the B chain (see below). DHFR-ricin is only 4-fold less toxic than ricin to L929 fibroblasts. It is also highly toxic to BW5147 lymphocytes (8-fold less than ricin).
|
To examine the possibility
that DHFR-ricin toxicity resulted from processing of DHFR-RTA within
cells to generate RTA, which would then be free to translocate to the
cytosol, we studied the stability of DHFR-RTA within cells during
intoxication by DHFR-ricin. As shown in Fig.
4, 125I-DHFR-RTA is stable
upon BW5147 cells intoxication by 5 nM
125I-DHFR-ricin, and no processing could be observed even
after 24 h of contact. Quantitative analysis of images obtained
from storage phosphor screens showed that beyond 24 h less
125I-DHFR-RTA was recovered from cells (Fig. 4). This
likely arose because more than 92% of cells have by then been killed
by 5 nM DHFR-ricin and consequently a proportion of cells
are nonpelletable by centrifugation.
Identical results have been observed when 125I-rRTA was taken up by cells in the form of free subunit or as 125I-recombinant ricin (L.M.R., unpublished). These results strongly indicate that, during intoxication by DHFR-ricin, DHFR-RTA does not break down to generate free rRTA by proteolysis. Together with the toxicity of DHFR-ricin we conclude that rRTA can carry DHFR into the cytosol.
DHFR-Ricin Cytotoxicity Is Inhibited by MTXTo examine whether unfolding of DHFR is required at any stage during intoxication by DHFR-ricin, MTX was used. However, as experienced by others, it was found that MTX alone interfered with the cytotoxicity assay (18), and we could not use concentrations significantly higher than the IC50 (10-30 nM) of MTX. Nevertheless 10 nM MTX was found to slightly protect BW5147 cells (1.5-3-fold) against intoxication by DHFR-ricin but did not protect against recombinant ricin (Table I).
Examination of the intoxication curves revealed that, at a DHFR-ricin
concentration below its IC50, 5 nM MTX had a
strong protective effect (Fig. 5),
whereas MTX provided no protective effect when recombinant ricin was
added to cells (not shown). We believe that protection by MTX is more
efficient at low DHFR-ricin concentration because the ratio
[MTX]/[DHFR-RTA] is higher and might ensure the binding of MTX to
DHFR even in intracellular vesicles where, as a result of endocytosis,
DHFR-RTA is more concentrated compared with MTX.
Endocytosis of DHFR-Ricin
Cell intoxication by toxins
is a multistep procedure and before drawing any conclusions from
cytotoxicity data it was necessary to determine at which stage MTX was
interfering with the DHFR-ricin intoxication process. To study binding
and endocytosis, the initial steps of the intoxication process, cells
were labeled with ricin-tetramethylrhodamine isothiocyanate and
DHFR-ricin-FITC for 30 min at 37 °C before lactose scraping,
fixation and examination under a confocal microscope (Fig.
6). Ricin was essentially intracellular,
in transferrin-positive endosomes (not shown and ref. 6) and 50 nM MTX did not affect uptake. In the absence of MTX,
intracellular DHFR-ricin labeled the same intracellular elements
as ricin.
Nevertheless, most DHFR-ricin molecules were plasma membrane-bound and insensitive to lactose scraping, suggesting that this chimera also interacted with the cell surface via its DHFR moiety. When MTX was present during DHFR-ricin endocytosis, most cell-surface labeling was displaced by the lactose washes, as observed for ricin (Fig. 6). Identical results were obtained using radiolabeled toxins and a conventional (6, 28) internalization assay (not shown). These data show that DHFR-ricin cell surface binding at 37 °C is mediated not only by its RTB (i.e. lactose sensitive), but also by its DHFR moiety (i.e. MTX sensitive). The ability of MTX to prevent this DHFR-mediated binding suggests that this interaction normally requires unfolding of the fused DHFR.
Does MTX affect internalization of DHFR-ricin? Neither confocal microscopic examination (Fig. 6), nor endosome fractionation (not shown) provided any evidence for this. We conclude that DHFR-ricin binding via DHFR is not significantly involved in its internalization, which is largely directed by RTB in a process which is insensitive to MTX. These observations are in complete agreement with the cytotoxicity data (Table I).
Translocation of DHFR-Ricin in Intact Cells Is Inhibited by MTXWe first examined the action of MTX on the translocation rate
of DHFR-RTA using cytotoxicity assays. Previous studies have shown that
the kinetic profile of protein synthesis inactivation by ricin consists
of an initial lag followed by a (pseudo)first order decrease in protein
synthesis. This slope directly reflects the membrane translocation rate
of RTA (5, 32). Using BW5147 lymphocytes, a
concentration-dependent lag was observed before the onset
of protein synthesis inactivation by recombinant ricin (Fig.
7A), as reported earlier for
ricin in other cell types (32). This lag period (also observed during
DHFR-ricin intoxication, Fig. 7B) is disregarded when
comparing the slopes of such inactivation curves (5, 32).
At all concentrations of recombinant ricin tested, 50 nM MTX did not alter the rate of protein synthesis inactivation (Fig. 7A). This MTX concentration did, however, affect cell killing by 3 nM DHFR-ricin (Fig. 7B). Here, the rate of cell killing is decreased almost 3-fold from 1 log/12 h to 1 log/31 h, showing that DHFR-RTA translocation is significantly inhibited by MTX. Similar results were obtained using L929 cells (data not shown).
Cell-free Translocation of DHFR-Ricin Is Blocked by MTXTo
study more selectively the inhibition of DHFR-RTA translocation by MTX
we made use of the only cell-free system enabling to follow ricin
translocation across intracellular membranes. This assay uses endosomes
purified from lymphocytes labeled with 125I-ricin (6). As
reported earlier for native ricin (6), when translocation of
radiolabeled recombinant ricin was examined, both 125I-rRTA
(30 kDa) and 125I-RTB (34 kDa) were transported through the
endosome membrane (Fig. 8A).
Background 125I-material present in the medium at the
beginning of the experiment was released during the last homogenization
step. Both ricin chains displayed the same translocation rate whether
translocation of recombinant (Fig. 8A) or native (6) ricin
was examined, demonstrating that rRTA behaves like native RTA in this
assay. Negative controls such as125I-transferrin, a
membrane-bound ligand, and 125I-horseradish peroxidase, a
soluble tracer, were not transferred through the endosome membrane
(6).
Translocation of 125I-DHFR-ricin proceeded at half the rate of 125I-recombinant ricin (not shown) indicating that 125I-DHFR-RTA translocation is below 10-15% of the efficiency of 125I-rRTA translocation. Most of the material transported through the membrane of 125I-DHFR-ricin-loaded endosomes was thus 125I-RTB whose translocation rate was unaffected whether 125I-RTB was endocytosed with 125I-rRTA or 125I-DHFR-RTA (not shown). These data are in agreement with our previous results demonstrating independent translocation of ricin chains (6).
The translocation rate of radiolabeled recombinant ricin was unaffected when as much as 10 µM MTX was added to the media (shown in Fig. 8B for 125I-RTA). When the same experiment was performed with 125I-DHFR-ricin-loaded endosomes, a complete inhibition of 125I-DHFR-RTA translocation by MTX was observed (Fig. 8B), whereas 125I-RTB translocation remained unchanged (not shown). These results indicate that, although the ability of MTX to cross biological membranes can be questioned (8), enough MTX molecules could enter the endosome lumen to affect DHFR-RTA transmembrane transport when 10 µM MTX was used in the cell-free assay. Translocation of DHFR-RTA was also selectively blocked when DHFR-ricin endocytosis and all subsequent steps were performed in the presence of 1 µM MTX (a concentration not affecting uptake over the labeling time) to ensure maximum endosome loading by MTX (not shown). The RNA N-glycosidase activities of DHFR-RTA and RTA, as checked on rabbit reticulocyte lysates, were insensitive to MTX up to 10 µM (not shown).
Altogether, these data provide compelling evidence that protection of cells by MTX from DHFR-ricin toxicity arises by selectively inhibiting the translocation of DHFR-RTA.
Unfolding is a general requirement for protein translocation into the ER (10), the mitochondria (9), or through E. coli plasma membrane (8). Appropriate targeting sequences fused to the N terminus of mouse DHFR has enabled translocation in these systems. Binding of the folate analogue MTX to the DHFR portion of these fusions prevents their unfolding and consequently their transport through the target membrane. The presence of NADPH (a cofactor of DHFR) was required in addition to MTX to block the translocation of a fusion protein consisting of DHFR connected to the precursor of outer membrane protein A (8), although this cofactor is not always essential.
Previous studies on DTA (14) and rRTA (15) translocation where the toxins were internally cross-linked by disulfidebridge engineering indicated that their unfolding was a prerequisite for translocation. A similar conclusion was reached using fusion proteins between DTA and the acidic fibroblast growth factor (17) or DHFR (18): a specific ligand impairing folding of the transported protein prevented translocation of the chimera.
There are a number of known differences between the translocation mechanisms of DTA and RTA (1, 2). One of these is the energy source, namely, direct ATP hydrolysis for RTA and the pH gradient (endosome-cytosol) for DTA (2, 5, 6). A further difference is the requirement for B chain to assist directly in the membrane traversal step. DTA translocation depends on the presence of the DT B chain-translocation domain, whereas for ricin, RTB can be readily replaced by other ligands (1). It was not therefore obvious that the requirement for unfolding in the case of DTA passenger proteins would also be relevant in the case of RTA.
Here we report the first successful use of RTA to transport a protein through membranes. DHFR-RTA, a fusion protein consisting of RTA fused via a spacer to the C terminus of DHFR, was produced while maintaining the biological activity of the parent proteins: both catalytic activities were apparent, and the propensity to associate with RTB remained unperturbed. The converse fusion, RTA-DHFR was produced in E. coli, but was unable to associate with RTB (data not shown). This was probably due to steric hindrance of the RTA C terminus which is normally involved in a number of non covalent interactions with RTB (33).
Using DHFR-ricin, a strong inhibition of membrane translocation by MTX was observed when studying inactivation curves of protein synthesis by intact cells. Unfortunately, the cytotoxicity of MTX alone limited the maximum concentration which could be used for these experiments to just 50 nM MTX. A complete arrest of DHFR-RTA translocation could be obtained using a cell-free ricin-translocation assay and micromolar MTX concentrations. These data indicate that unfolding of the passenger protein is required for translocation.
DHFR fusion proteins have been invaluable tools to study protein import into mitochondria (9, 34), and DHFR-RTA was therefore selected to provide information on RTA translocation intermediates. An unexpected observation from this study was the affinity of the ricin-fused DHFR for the cell surface in the absence of MTX. Such binding of DHFR has never before been reported. For most translocation studies, DHFR was targeted to intracellular organelles (8-11) and DHFR-DT binding to entire cells was examined at room temperature for 20 min (18), conditions unlikely to reveal interaction of fused DHFR with the plasma membrane, a process which requires over 10 min at 37 °C to become significant (not shown).
The ability of MTX to impair binding of ricin-fused DHFR to the cell surface stresses the point that drugs can affect the way cells handle toxin-conjugates at several stages. It is therefore necessary to separately examine all the steps involved in cell intoxication. Here, MTX was found to inhibit both the translocation of DHFR-RTA and the initial cell-surface binding of DHFR-ricin. Nevertheless, only the first of these MTX effects resulted in protection from DHFR-ricin. Furthermore, MTX did not affect cell-surface binding via RTB, which appears to be the only ligand allowing significant endocytosis of DHFR-ricin and subsequent cytotoxicity.
Truncated Pseudomonas exotoxin A efficiently introduced cellular viral epitopes in the cytosol of target cells for sensitization and lysis by peptide-specific CTLs (16). On the basis of our findings, enzymatically inactive rRTA would appear to be a suitable candidate to transport antigens into the cytosol for stimulation of cell-mediated immunity (19). A double codon mutation ensures virtually complete (i.e. > 1,000-fold) reduction in catalytic activity of RTA (35).
The CTL epitope (usually less than 20 residue long) could be envisaged as a fusion at the N terminus of this RTA mutant. It is known that the N-terminal residue is the only identified targeting signal for degradation via the ubiquitin-proteasome pathway. This cytosolic processing is required for subsequent transport of the epitope to the endoplasmic reticulum lumen followed by presentation at the cell surface by major histocompatibility complex class I molecules and activation of CD8+ CTLs (20). From the ability of DHFR-RTA to interact with RTB, an enzymatically-inactive rRTA with a short N-terminal extension should readily associate with RTB to ensure efficient endocytosis prior to delivery of the CTL epitope to the cytosol. The potential superiority of ricin over bacterial toxins for antigen delivery to the cytosol and CTL induction due to its higher number of cell surface binding sites (1) remains to be tested.
We are grateful to Drs. Richard Wales, Jean-Bernard Ferrini, John Chaddock, Paul Mangeat, and Philippe Montcourrier for help in various experiments and to Dr. Karin Becker (München, Germany) for her kind gift of anti-DHFR serum.