(Received for publication, March 14, 1997, and in revised form, May 9, 1997)
From the Laboratoire de Dynamique Moléculaire des Interactions Membranaires, CNRS UMR 5539, Université Montpellier II, Bât. 24, CC107, place Eugène Bataillon, 34095 Montpellier Cedex 5, France
Human recombinant ezrin, or truncated forms, were
coated in microtiter plate and their capacity to bind actin determined. F-actin bound ezrin with a Kd of 504 ± 230 nM and a molecular stoichiometry of 10.6 actin per ezrin.
Ezrin bound both - and
/
-actin essentially as F-form. F-actin
binding was totally prevented or drastically reduced when residues
534-586 or 13-30 were deleted, respectively. An actin binding
activity was detected in amino-terminal constructs (ezrin 1-310 and
1-333) provided the glutathione S-transferase moiety of
the fusion protein was removed. Series of carboxyl-terminal truncations
confirmed the presence of this actin-binding site which bound both F-
and G-actin. The F- and G-actin-binding sites were differently
sensitive to various chemical effectors and distinct specific ezrin
antibodies. The internal actin-binding site was mapped between residues
281 and 333. The association of ezrin amino-terminal fragment to
full-length ezrin blocked F-actin binding to ezrin. It is proposed
that, in full-length ezrin, the F-actin-binding site required the
juxtaposition of the distal-most amino- and carboxyl-terminal residues
of the ezrin molecule.
Proteins, located at the interface between the plasma membrane and the cytoskeleton, are essential elements involved in cell plasticity, and are expected to possess association properties tuned by both intra- and extracellular regulators. Ezrin is a protein linker between the cortical skeleton and the plasma membrane (1, 2), and, in polarized epithelial cells, colocalizes with actin predominantly in apical microvilli (1, 3-10). With talin, ezrin is part of the superfamily of protein 4.1-like proteins sharing a homologous NH2-terminal domain (11-14). With radixin (15, 16) and moesin (17, 18), which share ~70% homology, ezrin define the ERM1 family to which merlin (19, 20), is also related. Ezrin NH2-terminal domain is reported to interact with the plasma membrane, while the COOH-terminal domain would link the actin cytoskeleton (21). In some cell types, ERMs associate with CD44, a transmembrane receptor for hyaluronan (22) through regulation by the rho GTPase pathway (23).
In multicellular organisms, the relative level of ERM expression is tissue specific (4, 16, 24-28). However, ERMs are coexpressed and play a redundant role in most cell lines since major phenotypic alterations were only observed when the expression of all three ERMs was down-regulated (29). The subcellular redistribution of ERMs upon cell has been best studied in gastric parietal cells (2, 8). The elongation of ezrin-enriched secretory microvilli is linked to ezrin phosphorylation on serine and threonine residues (30), and ezrin acts as a protein kinase A anchoring protein in these cells (31). Ezrin is also tyrosine-phosphorylated (32-35) on two major sites (36) and a differential sensitivity to various growth factors exists between ERMs (37).
Ezrin can self-associate and, through interactions between two domains,
the N- and C-ERMADs, form dimers or oligomers, a property shared by
other ERMs, with which ezrin can form heterotypic associations (38-43). A COOH-terminal epitope is normally inaccessible in the molecule. Since this epitope is part of the C-ERMAD (42) and is
involved in F-actin binding (44), the masking of this site by
self-association, between monomers or within dimers, has been emphasized. Up to now little, and, sometimes, contradictory information were reported on the nature of interaction existing between actin and
ERMs. Radixin was characterized as a barbed-end capping protein (45).
Shuster and Herman (46) claimed that ezrin-actin interaction could not
be direct. On the contrary, using a column-affinity assay, Turunen
et al. (44) identified a F-actin-binding site within ezrin
35 COOH-terminal amino acids. This conclusion was enlarged to moesin
(47). Finally, gastric parietal cell ezrin was found to colocalize and
to preferentially interact with /
-actin (48, 49).
Our attempts to demonstrate a direct ezrin-actin interaction using experimental procedures similar or derived from those described above were unsuccessful. We therefore developed a new assay. Human recombinant ezrin purified from Escherichia coli was coated in wells of a microtiter plate. After incubation with actin, the specifically bound proteins were recovered and analyzed by SDS-PAGE and Western blotting. Ezrin, and a few ezrin recombinant constructs, bound F- and G-actin and the existence of a new actin-binding site in the NH2-terminal domain of ezrin was identified.
Chemicals, glutathione-agarose beads, and thrombin were from
Sigma, and antiprotease tablets from Boehringer Mannheim. Anti-actin monoclonal antibody was a gift of Dr. N. Lamb (Montpellier). Anti -actin antibody and all secondary antibodies were from Sigma. A
monoclonal antibody directed against ezrin residues 576-586 was from
Transduction Laboratories and was equivalent to the ZO36 antibody used
in Ref. 42.
Rabbit muscle acetone powder was
prepared and actin purified from as in Ref. 50. Actin was isolated as
CaATP-G-actin after cycling through 2-4 rounds of
polymerization-depolymerization prior to gel permeation chromatography
on Sephacryl S-300 in G buffer (2 mM Tris-HCl, pH 8.0, 0.2 mM ATPNa2, 0.2 mM
CaCl2, 0.005% NaN3, and 0.5 mM
-mercaptoethanol). Occasionally, actin purified by chromatography on
DEAE-Trisacryl was used and behaved similarly for ezrin-actin
interaction (not shown). A single 43-kDa band was stained by Coomassie
Blue when 30 µg of actin was loaded on SDS-PAGE. Actin was stored
either as G- or F-actin at 4 °C. After 3 weeks of storage, F-actin
was depolymerized, centrifuged for 2 h at 200,000 × g, and chromatographed again on Sephacryl S-300. Binding
studies involving G-actin were always performed with freshly gel-sized
G-actin.
Outdated platelets were obtained
from the CRTS (Montpellier, France). /
-Actin was purified on
polyproline-Sepharose (51) and eluted with 0.5 M KI (52),
dialyzed, polymerized, and stored in liquid nitrogen. A single 43-kDa
band was obtained after Coomassie Blue staining of 7 µg of protein
analyzed by SDS-PAGE.
The subcloning of the full-length
human ezrin cDNA into the pGEX-2T vector (Pharmacia Biotech Inc.)
was described (38) (Fig. 1, A and B).
Carboxyl-terminal deletions were obtained using the double stranded
nested deletion kit from Pharmacia. The ezrin-pGEX-2T vector was
cleaved with EcoRI and blunted with thio dNTPs. The plasmid
was cleaved with EspI to eliminate the codons corresponding to ezrin 53 COOH-terminal residues (Fig. 1D). Alternatively,
internal NH2-terminal deletions were performed after
NcoI digestion, filling with thio-dNTPs, and a final
digestion with BglII. This procedure eliminated the codons
corresponding to residues 12-114, favoring further deletions from the
115th position (Fig. 1C). In either case, exonuclease III
was added and the reaction stopped every 30 s for 10 min. After
nuclease S1 action, filling-in with the Klenow DNA polymerase fragment
and overnight ligation, TG1 bacteria were transformed with DNA.
Randomly picked clones were grown overnight in 3 ml of LB medium,
induced for 1 h with 0.5 mM
isopropyl--D-thiogalactopyranoside, sonicated, and
recombinant fusion proteins adsorbed on glutathione-agarose and
analyzed by Western blotting with anti-ezrin and/or anti-GST antibodies. Clones were selected according to the level of protein expression and mobility in SDS-PAGE, and inserts were sequenced using
the T7 Sequenase version 2.0 (Amersham). The full-length ezrin
cDNA, and the 5
-deleted clones, used its own stop codon. The
pGEX-2T vector provided the stop codon for carboxyl-terminal truncated
proteins. As a consequence 2 to 7 additional amino acids were encoded
by the vector and COOH-terminally added.
The replacement of RRRK (293-296) by LTGN was performed by
site-directed mutagenesis.2
Ez13-30 was obtained after digestion by NcoI
and BclI and filling-in with Klenow enzyme. The constructs
expressing ez310-586 and ez280-586 were
described (38).
A single
colony was used to seed 1 liter of LB medium. After 12-14 h growth,
0.5 mM isopropyl--D-thiogalactopyranoside
was added for 1 h and bacteria pelleted at 2500 × g for 10 min. The bacterial pellet was resuspended in 15 ml
of ice-cold phosphate-buffered saline containing 1 mM EDTA,
1 mM phenylmethylsulfonyl fluoride, and half a tablet of
protease inhibitor mixture. After sonication, the lysate was diluted to
45 ml with phosphate-buffered saline and centrifuged at 40,000 × g for 30 min at 4 °C. To the supernatant, 1 ml of
glutathione-agarose was added and the gel suspension left on a rotary
shaker for 18 h at 4 °C. The gel suspension was poured in a
column, washed with phosphate-buffered saline, and equilibrated in 50 mM Tris-HCl, pH 8.0. Full-length ezrin and constructs
expressing the carboxyl moiety of ezrin were eluted with 10 mM glutathione. Eluted proteins were dialyzed 18 h
against 20 mM Tris-HCl, pH 7.4, 0.1 mM EDTA,
and 13 mM
-mercaptoethanol. Proteins corresponding to
the NH2-terminal moiety of ezrin were eluted from
glutathione-agarose columns with 5 IU thrombin in 50 mM
Tris-HCl, pH 8.0, 150 mM NaCl, 2.5 mM
CaCl2, 1 mM dithiothreitol. After elution, 100 µl of p-aminobenzamidine-agarose beads (Sigma) were added.
Fusion proteins containing ezrin COOH-terminal parts were cleaved with
thrombin as described above before use. Thrombin was trapped on
p-aminobenzamidine-agarose beads and the GST moiety was
removed by adsorption on glutathione-agarose beads. After dialysis and
thrombin cleavage, full-length ezrin was purified on a Mono-Q FPLC
column equilibrated with 25 mM MES, pH 6.2, 20 mM NaCl, and 0.01%
-mercaptoethanol. Ezrin eluted with
45 mM NaCl. All proteins were stored in the presence of
0.05% NaN3 at 4 °C.
Ezrin (0.25 to 5 µg) was coated overnight in 96-well plate (Nunc) in 75 µl of 20 mM Tris-HCl, pH 7.4, 100 mM KCl, 5 mM MgCl2, and 0.2 mM dithiothreitol (F buffer) in a cell culture incubator at 37 °C. Wells were rinsed twice and residual binding sites blocked with 2% bovine serum albumin in coating buffer for 1 h at room temperature. Wells were rinsed three times and F-actin (0.1-10 µg; 50 nM to 5 µM) added in 50 µl of the same buffer containing 0.5 mM ATP and 2 µM phalloidin. When G-actin was added, wells were rinsed three times with G buffer (0.2 mM Tris-HCl, pH 7.4, 2 mM MgCl2, 0.2 mM dithiothreitol) and incubation was carried out in the G buffer. After 1 h incubation at room temperature, wells were rinsed rapidly 4 times with the corresponding incubation (F or G) buffer and 30 µl of 1 × SDS-PAGE sample buffer (54) were added to each well. Plates were incubated 20 min at 65 °C and the content of each well transferred in an Eppendorf test tube. Samples were heated to 100 °C for 3 min and run on a 10% SDS-PAGE. Gels were transferred using a semi-dry blotting technique on Immobilon P (Millipore).
Using an overnight coating procedure, ezrin binding on plastic was found a saturable and reproducible process. Half-maximum coating was achieved with 0.5-1 µg of ezrin. The maximum amount of ezrin coated was 50-100 ng (i.e. 0.7-1.4 pmol), a value similar to what announced by the manufacturer for IgGs. Therefore, the average ezrin density was estimated in the range of 2.5-5 pmol/cm2 (1 molecule/3000-6000 Å2). Thus, ezrin molecules could be evenly spaced on plastic and coating was not formerly dependent upon a self-association process although this cannot be definitively ruled out. 50 ng of coated ezrin led to a final ezrin concentration of 15 nM during the incubation step with actin. When different constructs had to be compared, 0.01 OD 280 nm of each construct was used for coating. Although all constructs had different molar extinction coefficient, this corresponded to an amount of protein larger than the one for which saturation of coating had been demonstrated with full-length ezrin. After quantification, the number of truncated molecules coated was found very similar. For the shortest constructs, at most a 2-fold increase in the number of molecules coated was measured when compared with full-length ezrin. Therefore, for these constructs, the actin binding ability would then be overestimated if no correction was introduced. This is of no consequence since they were actually found unable to bind actin (see "Results").
Quantification ProceduresImmobilon P membranes were stained with Coomassie Blue to check the efficiency of the transfer (5-10 ng of ezrin or actin could be detected). Western blotting was used to identify the bands, using either rabbit anti-ezrin or mouse anti-actin antibodies and appropriate peroxidase-labeled secondary antibodies. The ECL (Amersham) detection kit was used for the revelation. In some experiments, the Vistra ECF amplification module (Amersham) was used instead in conjunction with second antibodies labeled with alkaline phosphatase and blots scanned with a STORM PhosphorImager (Molecular Dynamics). Autoradiograms (ECL) and Coomassie Blue-stained membranes were scanned using a 8-bit CCD camera and the data digitized. In all cases, the ImageQuant software (Molecular Dynamics) was used to quantify the data. Calibrations were performed by running on separate gels serial dilutions of known amounts of ezrin and actin. These gels were processed similarly as the samples. When series of NH2- or COOH-terminal deletions had to be compared, their molar amounts were determined. It was assumed that the epitopes recognized by a mixture of antibodies recognizing either full-length ezrin or its NH2-terminal domain were evenly localized along the ezrin molecule. Thus the detected signal was corrected for the number of amino acids of each construct.
Anti-ezrin AntibodiesAnti-ezrin rabbit antibodies against full-length ezrin or its NH2-terminal domain (amino acids 1-310) were characterized by Andréoli et al. (38) and will be referred to as anti-C and anti-N antibodies, respectively. Antisera were used as such for Western blotting. For competition binding assays, both antisera were affinity purified on resins covalently coupled with the proteins used for immunization.
A summary of the
constructs used in this study is presented in Fig.
1. Constructs were produced as GST fusion
proteins (Fig. 1, A-D), purified, cleaved from the GST
moiety, and characterized by Western blotting with anti-ezrin
antibodies (Fig. 2).
Ezrin Is a F-actin-binding Protein
A new procedure to assay
actin-ezrin interaction was developed by analogy with enzyme-linked
immunosorbent assay tests (see "Materials and Methods"). A 96-well
plate was coated with purified full-length or truncated forms of ezrin
or the respective GST fusion proteins (Fig.
3). After incubation with /
-
(left panel) or
- (middle panel) F-actin and
subsequent washings, bound proteins were analyzed by Western blotting.
When GST was coated as a control, no actin was ever found bound.
However, a significant binding was reproducibly detected with some
specific ezrin constructs. Full-length ezrin (ez1-586)
bound actin. Interestingly, ez1-310 also bound actin,
whereas the respective GST fusion protein never did. GST as a fusion
protein with NH2-terminal forms of ezrin did lower or even
prevent actin binding to chimeric ezrin molecules, and this effect was
also observed when
-actin was used (not shown). Therefore, for all
subsequent studies, only recombinant proteins, from which the GST
moiety was cleaved, were used.
-Actin also bound full-length ezrin and other ezrin constructs (Fig.
3, middle panel). Western blots with anti-ezrin and anti-actin antibodies indicated the relative levels of construct coating and actin binding in each well, respectively. In Fig. 3
(right graph), the same data were normalized to allow a
quantitative comparison and shown for two actin concentrations.
Ez1-586 bound
-actin. Deleting the 53 COOH-terminal
amino acids (ez1-533) severely impaired actin binding.
This was consistent with previous results (44, 47). However, no binding
of actin to ez310-586, i.e. the COOH-terminal
part of ezrin containing the previously identified actin-binding site
was detected under these assay conditions, whereas ez1-310
did significantly bind actin. When amino acids 13-30 were deleted
(ez
13-30), actin binding was drastically reduced. These
residues were deleted because they were found important to regulate the
cell extension phenotype observed in insect cells.2
Overall, the presence of a binding site in the NH2 terminus
domain of ezrin was therefore consistently detected whatever the actin source.
In this assay, actin
nonspecific binding was undetectable and binding to holo-ezrin was
saturable (Fig. 4, A and
B). Heating ezrin to 65 °C for 30 min before the coating
step led to a large decrease in actin binding capacity (Fig.
4B), although heat denaturation drastically enhanced ezrin
coating (see "Discussion"). Ezrin storage at 20 °C led to a
complete loss in actin binding (not shown). The threshold concentration
of
-actin required to achieve a significant binding was 50 nM (2 µg/ml). Half-saturation was reached at 20 µg/ml
-actin (0.5 µM, 1000 ng of actin added). Scatchard
analysis (Fig. 4A, inset) demonstrated that actin interacted
reversibly with a homogenous population of ezrin molecules, with a
Kd value of 504 ± 230 nM
(n = 13) and a stoichiometry of 10.6 actin monomers
bound per ezrin molecule (range 6-18.4).
Mapping of Actin-binding Sites
Actin binding was analyzed with all constructs described in Figs. 1 and 2. The results were compared for two actin concentrations after quantification and normalization (Fig. 1). The data were fully consistent with those described in Figs. 3 and 4. The short deletion of amino acids 13-30 drastically reduced actin binding (Figs. 1, 3 and 4B). Extending the deletion to residue 115, and beyond up to position 507, completely abolished actin binding. On the other hand, the truncation of ezrin COOH-terminal last 53 amino acids totally impaired actin binding while shorter constructs, ez1-310 and ez1-333, did bind actin, ez1-333 being more efficient that ez1-310 (Fig. 1). A further increase in size to position 366 led to a sudden loss in actin binding. This suggested that a small number of amino acids beyond position 333 were sufficient to negatively control the binding observed with shorter NH2-terminal ezrin fragments. COOH-terminal truncations of ez1-310 led also to a loss in actin binding as shown with ez1-280 and shorter constructs. As already noted above, ezrin COOH-terminal domains containing the previously published F-actin-binding site were incapable of binding (ez310-586 and ez280-586). Finally, the mutation of amino acids 293-296 from RRRK to LTGN in ez1-310 led to a significant drop in actin binding which was consistent with the demonstration that the same mutations in ez1-310 impaired actin-based cell extension in Sf9-infected cells.2
Full-length Ezrin and Ezrin NH2-terminal Domain Discriminate between F- and G-actinThe data suggested that ezrin
might contain two distinct actin-binding sites with distinct
properties. For instance, ez1-586 and ez1-333
bound F-actin with different stoichiometry (Fig. 4B). The
Kd of ez1-586 for G-actin (710 nM, n = 4) was similar to the value
determined for F-actin (see above and Fig. 4E). However, the
binding capacity was markedly different since the ratio of actin
monomer bound per ez1-586 was 0.9 (range 0.75-0.94). With
ez1-333, large variations were observed (see
"Discussion"). Ez1-333 bound 0.27-1.0 G- and 0.7-6.0 F-actin molecules (in paired experiments, the F-actin binding capacity
was 3-5 times that of G-actin). The Kd values for
F- and G-actin binding to ez1-333 were similar,
respectively, 205 (n = 5) and 280 (n = 3) nM. The comparison between ez1-586, ez1-333, and ez1-310 to bind either F- or
G-actin showed indeed selective differences (Fig.
5A). Ez1-310
bound equally F- and G-actin, while ez1-586 and
ez1-333 were more efficient in binding F-actin.
To better distinguish these different sites, the actions of various effectors were tested. NaCl addition did not alter F-actin binding to ez1-586 or ez1-333 (Fig. 5B). Using 40 µg/ml G-actin, increasing NaCl concentration resulted in enhanced actin binding, consistent with actin polymerization triggered by salt addition (Fig. 5B, right) and recovery of pelletable actin from the wells. At 4 µg/ml G-actin, a value below the critical G-actin concentration required for polymerization (55), the binding was independent of ionic strength (Fig. 5B, left). Therefore, the G-actin binding appeared to be initially due to the G form of actin. Whether this initial binding led to nucleation sites remains to be determined.
N-Ethylmaleimide treatment of ez1-586 or
ez1-333 had no effect on either F- or G-actin binding
(Fig. 5C). Succinimidyl-6-(biotinamido)hexanoate addition
reduced F-actin binding to ez1-333 only, while 3,3-dithio-bis(propionic acid N-hydroxysuccinimide ester)
treatment abolished F-actin binding of both constructs. None of these
compounds significantly altered G-actin binding to either protein (Fig. 5C). Therefore, the regions for both ezrin constructs
involved in F-actin binding shared similar sensitivities to these
effectors, and were clearly distinct from those involved in G-actin
binding.
Next, the effects of different affinity purified antibodies were tested on the binding of F- or G-actin to ez1-586 or ez1-333 (Fig. 5D). The anti-C antibody did not recognize the NH2-terminal ezrin domain (positions 1-310) (21, 38).3 Anti-N and anti-C antibodies bound both ez1-586 and ez1-333. Anti-C antibody bound much better onto holo-ezrin than onto ez1-333, and subsequent addition of either F- or G-actin did not interfere with antibody binding (not shown). No major effect of anti-N and -C antibodies was detected on F-actin binding to ez1-586. However, anti-N antibody interfered slightly with F-actin binding to ez1-333. At high concentrations, anti-N antibody reduced G-actin binding to ez1-586, while both anti-N and -C antibodies, alone or in combination, blocked G-actin binding to ez1-333. A commercial monoclonal antibody directed against amino acids 576-586 failed to bind coated ez1-586 and to block actin-binding (data not shown). Therefore, it was concluded that: 1) the F-actin-binding sites on either ez1-586 or ez1-333 was distinct from the G-actin-binding site; and 2) the G-actin-binding site detected in ez1-333 should involve residues 281-310, the only stretch of residues accessible to anti-N antibodies and residues 311-333 accessible to C blocking antibodies.
NH2-terminal Fragments of Ezrin Associate with Ezrin and Block Actin BindingAt variance with the results of Turunen
et al. (44), no F-actin binding was detected with ezrin COOH
terminus constructs (Figs. 1 and 3). To test if this failure resulted
from the coating procedure, ez310-586 was added in the
assay as a competitor of F-actin binding to coated
ez1-586. The competitive action of ez1-310
was also compared (Fig. 6, left
panel). Whether F-actin was added alone or in combination with
ez310-586, no differences were observed for actin binding
to ez1-586. On the contrary, when ez1-310 was
added together with F-actin, actin binding to
ez1-586 was drastically reduced.
Ez1-310 appeared as a strong competitor for actin
binding to ez1-586. However, ez1-310 was
recovered from wells suggesting that an association between
ez1-310 and ez1-586 took place during the
assay. The possibility that such interaction was responsible for the
observed loss of actin binding was next tested using
ez1-333 which behaved similarly (Fig. 6, right
graph). When ez1-333 was added after F-actin had
bound to ez1-586, it still associated with ezrin (not
shown) but did not affect much actin binding. Simultaneous addition of
actin and ez1-333 led to a dramatic drop in actin binding.
Incubation of ez1-333 prior to actin addition resulted in
an almost complete block in actin binding. Ez1-333 binding
to ez1-586 was independent of the presence of bound actin,
whereas the presence of ez1-333 did prevent actin binding
to full-length ezrin.
The major result of this paper is the description of the actin-binding properties of recombinant ezrin using a solid phase assay and the characterization of important determinants within ezrin NH2-terminal domain involved in actin binding.
Validity of the AssayThe assay was specific on different
criteria. Actin never bound to proteins such as bovine serum albumin or
GST. Ezrin and actin interacted within a reasonable range of
concentration: in the nanomolar range for ezrin; saturation was
achieved with micromolar actin concentrations. Among 24 ezrin
constructs, only four, including ezrin, bound actin. One of them was
deleted of a few amino acids (ez13-30) and showed very
reduced binding. The two others are very similar constructs and only
differ by a 23-amino acid extension (ez1-310 and
ez1-333). The effects of various compounds (Fig. 5) and
competitors (Fig. 6) strongly argue for the selectivity and specificity
of binding events.
The conformation of the coated proteins is an important issue. Some change(s) did occur in ezrin since the soluble form was unable to bind actin in standard biochemical assays. Did coating promote ezrin denaturation or self-association? Heat denaturation increased coating by a factor of 10 (not shown), whereas the resulting normalized actin binding was low (Fig. 4B), and presumably represented binding to ezrin molecules that had either escaped denaturation or renatured during overnight coating. The enhancement of coating reflected aggregation of ezrin molecules after heat treatment. Therefore, when native ezrin was coated, if self-association took place, it should have remained minimal. The antibody directed against residues 576-586 failed to interact with ezrin coated on plastic, a result consistent with the C-ERMAD being not accessible in full-length ezrin unless the protein is SDS-treated (42). Altogether the data supported that ezrin was coated as a majority of monomers, in a configuration distinct from soluble ezrin, but also distinct from heat-denatured, thawed, or blotted ezrin. Since the presence of intact both C- and N-ERMADs (42) is necessary to achieve oligomerization, all other constructs tested could not self-associate.
Discrepancies with Other StudiesThe ezrin-actin interactions
are not yet fully understood. Conflicting results were reported,
probably because different sources of ezrin, with various
post-translational states, and different assays were used. Soluble
gastric ezrin discriminated between /
- and
-actin isoforms
(48, 49). On overlay assay, blotted ERMs bound F-actin but not G-actin
(47). The truncation of the 22 COOH-terminal residues in GST-moesin
abolished actin binding. Turunen et al. (44) used an
immobilized GST-ezrin COOH-terminal domain to demonstrate that the last
35 amino acids of the molecule are required for F-actin binding. Using
GST-fusion proteins, no binding was detected in ezrin (44) and moesin
(47) NH2-terminal domains. We used recombinant proteins
from which the GST moiety was cleaved. A free NH2-terminal
extremity is of critical importance for unmasking ezrin
NH2-terminal actin-binding properties. In this respect,
Henry et al. (56) stressed the importance of proper radixin
tagging for correct cellular addressing. SDS treatment is a
prerequisite of any overlay assay (47) and Turunen et al. (44) mentioned that a SDS step was necessary to solubilize
NH2-terminal constructs. Since SDS treatment inactivated
the association properties of ezrin N-ERMAD (42), it might have also
affected actin binding in previous studies.
The deletion of amino acids 13-30, a sequence most probably hindered by the GST moiety in fusion proteins, considerably reduced actin binding, and this result further strengthens the importance of ezrin NH2-terminal residues in actin binding. Actually, ez1-310 and ez1-333 were much more efficient than any COOH-terminal ezrin construct to bind actin. One should note, however, that some actin binding was detected in COOH-terminal constructs, but this required that, at the ECL reaction step, films were exposed much longer (15 min instead of 30 s) (not shown). It is possible that, when C-domains were coated, the F-actin-binding site stayed masked or was involved in interaction with plastic. In both cases it would be inaccessible to actin. However, the soluble form of the C-domain did not act as a competitor either (Fig. 6). Then it is possible that this site is of much lower affinity than was earlier anticipated and that it cannot be detected in this type of assay. Actually, no Kd value was previously measured for ezrin C-domain.
Albeit, apparently 10-fold higher, the Kd (500 nM) for F-actin determined in this study agrees with the
value measured by Yao et al. (48). Their analysis was
performed in the reciprocal way keeping actin concentration constant
and varying that of ezrin. Therefore, the slope of the Scatchard was
expressed in nM1 ezrin (Kd = 50 nM). In both studies, one ezrin molecule bound every
10 actin monomers. Either due to steric hindrance or because of actin
conformational change due to ezrin binding, the actual actin binding
motif is an actin decamer. Thus, the affinity of actin for ezrin was
either 500 nM relatively to the full population of actin
molecules or 50 nM when the decameric actin binding motif
is considered.
The low stoichiometry (<1.0) between G-actin and ez1-586 or, especially, ez1-333 suggested that either some actin was lost during the final washing steps due to a high dissociation rate or some uncontrolled aggregation occurred during coating, especially with NH2-terminal constructs of low solubility. If resulting aggregates were unable to bind actin, the measured stoichiometry would be lowered accordingly. Alternatively, the G-actin-binding site might be a nucleation center. Such a process to be effective would then concern only those of ez1-333 molecules which were close enough to bring three actin molecules together.
Implications for Ezrin FunctionOur attempts to demonstrate ezrin-actin interactions using conventional procedures failed except when ezrin was immobilized on a solid phase. It is tempting to speculate that adsorption to plastic mimics anchoring to the plasma membrane. This is consistent with a recent model describing soluble ezrin in a dormant state (39). Similarly, the re-association of ezrin COOH- and NH2-terminal domains was never possible in solution, whereas, it is very efficient using either overlay assays (38, 41-42) or surface plasmon resonance technology,4 i.e. after immobilization of one partner.
The observation that the NH2-terminal domain possesses
actin-binding properties justifies to reconsider previous models of ezrin function. Deletions of ezrin NH2-terminal residues
(1-30 or 13-30) or truncation of the COOH-terminal last ones
(566-586) promoted a cell extension phenotype with concomitant actin
mobilization in Sf9 cells.2 For ez13-30 or
COOH-terminal truncated forms, actin binding was either reduced or
abolished. Thus, the F-actin-binding site could involve both the very
NH2- and COOH-terminal ends of the molecule implying a
folding process or the involvement of NH2- and
COOH-terminal ends of two distinct molecules to constitute the
actin-binding site. This interpretation is consistent with the
specificity of anti-ezrin antibodies (38, 21, 42), and their lack of
effect on F-actin binding (Fig. 5D). Whether ezrin molecules
are positioned head-to-tail or folded, there exists an additional site
within amino acids 281-333 as previously suggested (21). It exhibits
distinct properties toward F- and G-actin (see Figs. 4 and 5), and we
propose that it actually represents a G-actin-binding site. Amino acids
334-366 prevent the accessibility of this site and this further
suggests that a folding event occurs within full-length ezrin masking
this G-actin site (there is no evidence that upon F-actin addition this
internal G-actin site is still accessible). Such a head-to-tail
interaction exists in vinculin and prevents actin binding. Addition of
phosphatidylinositol 4,5-bisphosphate released this conformation and
allows actin and talin binding (57). Such a mechanism, controlled by
intracellular signaling, may well be involved in ezrin for which a
phosphatidylinositol 4,5-bisphosphate-binding site is located in
ez1-310 (58). The association of ez1-310 or
ez1-333 with ezrin prevented F-actin binding to
full-length ezrin (Fig. 6). Such association would open the ezrin
molecule and the NH2- and COOH-terminal moieties would no
longer form the actin binding pocket.
The capacity for some forms of ezrin to bind G-actin is of physiological interest. It is important to determine if this site can be turned on/off in native ezrin, upon (de)phosphorylation, and whether it can act to regulate microfilament assembly/disassembly. The F-actin binding capacity of radixin and ezrin were similar (data not shown). Radixin acts as a barbed-end capping protein (45) which implies that one actin filament would bind one radixin molecule. Other studies concluded that ERM proteins bind along the sides of actin filaments (47, 49), a proposal consistent with the comparable amount of both ezrin and actin found in purified microvilli (2, 39), and with the stoichiometry determined in vitro (this paper and Ref. 49). Ultimately, both types of association may exist. One, along the sides of the filaments, would consist in a structural role and stabilize the cortical architecture. The second would act on actin barbed ends and would help regulate membrane extensions (53, 59, 60). We propose that the latter function is only possible by disruption of the former type of association.
In Sf9 cells,2 extensions promoted by ezrin NH2-terminal constructs, required the mobilization of tubulin, a situation reminiscent to what happened in physiological systems (60, 61). Preliminary in vitro experiments clearly showed that ez1-310 binds tubulin. Whether ezrin acts as a cross-talking protein between microfilaments and microtubules can now be tested directly using the solid phase assay. In cells, microfilaments and microtubules are of extreme importance to regulate membrane dynamics such as membrane movement at the cell leading edge, localization of new adhesion plaques, and guidance of axonal growth cone. Proteins involved in the cross-talk between the two filament systems have not yet been clearly described and it is of interest that ERM proteins might be potential candidates.
We are particularly indebted to Dr. M. Arpin for helpful discussion and communication of results prior publication. Dr. N. Lamb is thanked for providing anti-actin mouse monoclonal antibodies. D. De Rigal is thanked for help with the expression and purification of recombinant proteins. The digital processing of the data would not have been possible without the precious expertise of A. Sahuquet.