©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
The Role of the Transcription Factor Sp1 in Regulating the Expression of the WAF1/CIP1 Gene in U937 Leukemic Cells (*)

(Received for publication, August 31, 1995; and in revised form, October 31, 1995)

Joseph R. Biggs (1) Jeffery E. Kudlow (2) Andrew S. Kraft (1)(§)

From the  (1)Divisions of Hematology/Oncology and (2)Endocrinology, University of Alabama at Birmingham, Birmingham, Alabama 35294

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

The Waf1/Cip1 protein induces cell cycle arrest through inhibition of the activity of cyclin-dependent kinases and proliferating cell nuclear antigen. Expression of the WAF1/CIP1 gene is induced in a p53-dependent manner in response to DNA damage but can also be induced in the absence of p53 by agents such as growth factors, phorbol esters, and okadaic acid. WAF1/CIP1 expression in U937 human leukemic cells is induced by both phorbol ester, a protein kinase C activator, and by okadaic acid, an inhibitor of phosphatases 1 and 2A. Both of these agents induce the differentiation of these leukemic cells toward macrophages. We demonstrate that phorbol esters and okadaic acid stimulate transcription from the WAF1/CIP1 promoter in U937 cells. This transcription is mediated by a region of the promoter between -154 and +16, which contains two binding sites for the transcription factor Sp1. Deletion or mutation of these Sp1 sites reduces WAF1/CIP1 promoter response to phorbol ester and okadaic acid, while a reporter gene under the control of a promoter containing only multiple Sp1 binding sites and a TATA box is induced by phorbol ester and okadaic acid. The WAF1/CIP1 promoter is also highly induced by exogenous Sp1 in the Sp1-deficient Drosophila Schnieder SL 2 cell line. These results suggest that phorbol ester and okadaic acid activate transcription of the WAF1/CIP1 promoter through a complex of proteins that includes Sp1 and basal transcription factors.


INTRODUCTION

Treatment of the human myeloid leukemic cell line U937 with phorbol esters such as phorbol myristate acetate (PMA), (^1)an activator of protein kinase C, leads to macrophage/monocyte-like differentiation over a 72-h period(1, 2) . This process involves changes in cell-substrate adherence, growth arrest in late G(1), and increased expression of monocyte markers(3, 4) . Similarly, treatment of U937 cells with okadaic acid, a natural product isolated from the black sponge and a potent inhibitor of protein phosphatases 1 and 2A, also induces differentiation of these cells(5) , cell cycle arrest, and eventual (72-h) apoptosis(6) . Both PMA and okadaic acid induce expression of the cyclin-dependent kinase inhibitor, WAF1/CIP1(7, 8) .

WAF1/CIP1 expression is induced by the p53 protein following irradiation of cells(9, 10) , but p53-independent expression of WAF1/CIP1 is associated with differentiation of myocytes (11, 12) , of HL 60 leukemic cells(13) , and of a number of other cells. WAF1/CIP1 is expressed in a number of tissues over the course of murine development, and expression in most tissues is not dependent on the presence of p53(14) . p53-independent expression of the WAF1/CIP1 gene can be induced in cultured cells by a number of agents, including, besides PMA and okadaic acid, platelet-derived growth factor, fibroblast growth factor, and transforming growth factor beta(15, 16) .

Preliminary analysis of the WAF1/CIP1 promoter suggests that the elements mediating response to serum in fibroblasts are located at least 1.9 kb upstream from the transcription start site(14) , while responsiveness to tumor growth factor beta is mediated by elements located somewhere in the promoter sequences 1.3 kb upstream of the transcription start site(17) . We now report that two sites that bind the transcription factor Sp1, located approximately 115 and 65 base pairs upstream from the transcription start site of the WAF1/CIP1 gene, are necessary for normal levels of basal, PMA-induced, and okadaic acid-induced transcription. These sites are also necessary for induction of the WAF1/CIP1 promoter by exogenous Sp1 in the Sp1-deficient Drosophila Schneider SL 2 cell line. Finally, our observation that a reporter plasmid containing multiple Sp1 binding sites and a TATA box shows transcriptional induction in response to PMA and okadaic acid in U937 cells suggests that the activity of Sp1 is sufficient for induced transcription of the WAF1/CIP1 gene.


MATERIALS AND METHODS

Cell Culture and Conditions

U937 human myeloid leukemic cells obtained from ATCC (Rockville, MD) and Dr. D. Kirkways (East Carolina University, Greenville, NC) were passaged in Dulbecco's modified Eagle's medium (Life Technologies, Inc.) supplemented with 10% heat-inactivated bovine calf serum (Life Technologies, Inc.) and antibiotics at 37 °C in 5% CO(2). Drosophila Schneider SL 2 cells were grown at room temperature in Schneider's Drosophila medium (Life Technologies, Inc.) supplemented with 20% fetal calf serum.

RNA Isolation and Northern blot Analysis

Total cellular RNA was extracted by the guanidium thiocyanate/CsCl ultracentrifugation method. RNA was separated on 1.2% agarose gels containing formaldehyde and transferred to a membrane. To detect specific transcripts, P-cDNA probes labeled by random priming were hybridized to the membranes. The probes used were a 2.1-kb fragment containing an approximately full-length WAF1/CIP1 cDNA and a 1.5-kb tubulin cDNA.

Plasmids

The CAT reporter plasmid containing 2.3 kb of WAF1/CIP1 promoter sequence was a gift from T. Waldman and B. Vogelstein (Johns Hopkins University, Baltimore, MD). Smaller regions of the WAF1/CIP1 promoter were obtained by PCR amplification using the larger promoter construct as template and primers containing the desired 5` and 3` promoter sequences. Mutations introduced into 5` primers were incorporated into the subsequent constructs. PCR products were first cloned into the pCRII vector (InVitrogen) and then moved into the CAT reporter plasmid pJFCAT1 modified by excision of the SV40 trimer cassette(18) . The -122/-61 deletion contruct was generated by excising sequences between SmaI sites at -125 and -63 from the construct containing promoter sequences from -154 to +16. 2.2 kb of upstream WAF1/CIP1 promoter sequence was inserted into this -122/-61 deletion construct using an ApaI site at -129 and a vector HindIII site at the 5` end of the promoter sequence. The -81/-62 deletion was made by cloning the -154/+16 fragment of promoter into the single-stranded vector M13 mp18 and then using a primer containing sequences from either end of the desired deletion for site-directed mutagenesis. To construct the Sp1-dependent luciferase reporter plasmid pGAGC6, oligonucleotides containing consensus Sp1 binding sites flanked by BamHI and BglII restriction sites were synthesized. A multimer of this oligonucleotide containing six Sp1 binding sites was obtained by BamHI/BglI digestion followed by ligation. The DNA fragment containing the Sp1 multimer was then cloned into a luciferase reporter plasmid upstream of the adenovirus major late initiator TATA box, which had been previously cloned into the luciferase plasmid. The plasmid pGAM, containing only the adenovirus TATA box, was used as a control.

Transfection and Reporter Gene Assays

U937 cells were transfected by electroporation, and CAT assays were performed as described previously(19) . 24 µg of WAF1/CIP1 promoter construct and 1 µg of cytomegalovirus/beta-galactosidase plasmid as a transfection control were used per 10 million cells. After electroporation, cells were divided into three culture dishes and allowed to recover for 6 h in medium containing 10% bovine calf serum. One dish was then treated with 200 nM PMA and one dish with 100 nM okadaic acid. 24 h later cells were harvested and subjected to freeze-thaw lysis, and 75 µg of total cell protein was used for CAT assays. For SL 2 cells, 5 million cells in 60-mm dishes containing 1 ml of plain medium were transfected with 4 µg of reporter plasmid using Lipofectin. After 6 h, the Lipofectin was removed and replaced with medium containing 20% fetal bovine serum. For luciferase assays, cells were transfected as above, then lysed in 125 mM Tris, pH 7.8, 10 mM dithiothreitol, 10 mM trans-1,2-diaminocyclohexane-N,N,N`,N`-tetraacetic acid, 50% glycerol, and 5% Triton X-100. 125 µg of total cell protein in 100 µl of lysis buffer was mixed with 100 µl of luciferin buffer (20 mM tricine, 1.07 mM magnesium carbonate, 2.67 mM MgSO(4), 0.1 mM EDTA, 33.3 mM dithiothreitol, 530 uM ATP, 270 uM coenzyme A, and 470 µM luciferin, pH 7.8), and light intensity was measured.

Gel Mobility Shift

For gel mobility shift experiments, oligonucleotides containing the desired promoter sequences were synthesized, annealed, and labeled using T4 polynucleotide kinase. After polyacrylamide gel purification, the labeled oligonucleotides were incubated with 5 µg of U937 nuclear extract in a buffer containing 70 mM KCl, 20 mM Hepes, pH = 7.6, 1 mM dithiothreitol, 0.1 mM EDTA, 0.01 mM ZnCl(2), 60 µg/ml poly(dI-dC), and 30 µg/ml bovine serum albumin at 4 °C for 30 min. Nuclear extracts were made as described (20) in a buffer containing 25% glycerol. For experiments using antibodies, 1 µl of anti-Sp1 polyclonal antiserum (21) or preimmune serum was added to the protein extract plus buffer, and a 15-min preincubation on ice was carried out before the addition of radiolabeled oligonucleotide. For competition experiments, unlabeled competitor oligonucleotides were also added to protein extracts for a 15-min preincubation. The sequence of the control oligonucleotide lacking an Sp1 site used in Fig. 6is 5`-AGCCAGGAGCCTGGGCCCCGGGGAG.


Figure 6: Exogenous Sp1 stimulates transcription of the WAF1/CIP1 promoter in the Sp1-deficient Drosophila Schneider SL 2 cell line. 4 µg of the indicated WAF1/CIP1 reporter constructs were transfected into SL 2 cells either with (+) or without(-) 100 ng of the Sp1 expression vector, pPacSp1. The numbers below the Sp1 (+) lanes give average induction in response to Sp1 calculated from four independent transfection experiments.




RESULTS

Treatment of U937 cells with either PMA or okadaic acid induces accumulation of WAF1/CIP1 mRNA (Fig. 1). Treatment with 100 nM PMA results in maximum levels of RNA by 2-4 h, while cells treated with 100 nM okadaic acid do not accumulate maximal levels of WAF1/CIP1 mRNA until 8 h (Fig. 1). This difference in the rate of induction between these agents is based on the concentration of activator employed, since higher concentrations of okadaic acid, 500 nM, result in increases in the level of WAF1/CIP1 mRNA levels at earlier time points (Fig. 1).


Figure 1: Accumulation of WAF1 mRNA in response to treatment of U937 cells with PMA or okadaic acid. U937 cells were treated with 100 nM PMA, 100 nM okadaic acid, or 500 nM okadaic acid as indicated above the lanes. At the indicated times, cells were collected and used to prepare RNA for Northern blot analysis. 20 µg of total RNA was loaded in each lane, and filters were first probed with radiolabeled WAF1/CIP1 cDNA and then stripped and reprobed with tubulin cDNA as a loading control.



To determine whether PMA and okadaic acid stimulate transcription from the WAF1/CIP1 promoter, contructs containing varying lengths of the WAF1/CIP1 promoter in front of a CAT reporter gene were transfected into U937 cells. Each set of transfected cells was split into equal aliquots, which were treated with PMA or okadaic acid or left untreated as controls. The smallest construct, containing 170 base pairs of promoter sequence (from 154 bases upstream of the transcription start site at +1 to +16), was fully inducible by both PMA and okadaic acid (Fig. 2). When compared with the -2320/+16 construct (Fig. 2), the -154/+16 construct was more strongly induced by PMA (19.5- versus 7.3-fold) and okadaic acid (16.1- versus 9.8-fold), suggesting that upstream elements that repress transcription may be found between -2320 and -154. A series of intermediate constructs displayed a gradual increase in response as promoter sequence was deleted from -2320 to -154, but all constructs were induced by PMA and okadaic acid. (^2)The two p53 binding sites identified at positions -2.3 kb and -1.4 kb do not play any role in the induction by these two agents since they are deleted in the smaller constructs without any affect on transcription. Further analysis of promoter sequences downstream from -154 revealed that a deletion of promoter sequences between -122 and -61 eliminated both basal and inducible promoter activity (see Fig. 3). When the -122/-61 deletion is introduced into a construct containing 2.3 kb of upstream WAF1/CIP1 promoter sequence, induction of transcription in response to PMA or okadaic acid is lost (Fig. 2), demonstrating that these sequences are important for inducible transcription and cannot be replaced by upstream elements. These elements are also important for induction in response to p53. Although U937 cells contain no wild-type p53, cotransfection of a wild type p53 expression vector induces transcription from the undeleted 2.3-kb WAF1/CIP1 promoter (Fig. 2). The -122/-61 deletion, however, abolishes induction in response to p53. Similar results were obtained when the two promoter constructs were compared in the GM glioma cell line, which contains a wild type p53 gene under the control of a steroid-inducible promoter (data not shown). These results indicate that WAF1/CIP1 promoter elements within the -122/-61 region are necessary for both p53-dependent and p53-independent induction.


Figure 2: Promoter sequences between -122 and +16 mediate phorbol ester and okadaic acid induction of the WAF1/CIP1 promoter. A, diagrammatic representation of CAT constructs containing between 2.3 kilobases and 170 bases of WAF1/CIP1 promoter sequence. All constructs shown have a 3` terminus at +16, where +1 is the transcription start site. B, constructs were electroporated into U937 cells; one-third were treated with 200 nM PMA, one-third were treated with 100 nM okadaic acid, and one-third were untreated controls, as indicated below the panels. 24 h later CAT activity was assayed; for each construct, one assay is shown and average induction in response to PMA or OK calculated from three independent transfection experiments is listed below each lane. All transfections included cytomegalovirus/beta-galactosidase plasmid; cell extracts were assayed for galactosidase activity to ensure equal transfection efficiency (galactosidase assays not shown). C, U937 cells were cotransfected with the indicated WAF1/CIP1 promoter constructs and vectors expressing either wild-type p53 (WT) or mutant p53 (Mut). CAT activity was assayed 24 h after transfection.




Figure 3: Regions containing Sp1 consensus binding sites mediate promoter response to PMA and okadaic acid. A diagram of CAT contructs with WAF1/CIP1 promoter sequence deleted between -154 and -61 is shown in the upper part of the figure. The lower part shows CAT assays from U937 cells transfected with the WAF1/CIP1 promoter construct indicated above each panel and then split into three aliquots and treated with 200 nM PMA, treated with 100 nM OK, or left untreated(-) as indicated beneath the panels. The basal transcription and -fold activation numbers shown below are the averages of three independent transfection experiments. All experiments included one transfection with the -154/+16 WAF1/CIP1 promoter construct as a standard for basal activity. -Fold activation for each construct was calculated based on the basal activity of that particular construct.



The WAF1/CIP1 promoter sequence between -122 and -61 does not contain consensus binding sites for factors such as AP 1, Egr-1, or NF-kappaB, which are known to activate other genes in response to phorbol esters or okadaic acid(22, 23) . However, the region does contain several consensus binding sites for the transcription factor Sp1(7) . DNase I footprint analysis of this region indicated that at least two Sp1 consensus binding sites, centered around -115 and -67, fell within protected regions.^2 PMA treatment of cells did not cause any noticeable change in the footprints, suggesting that the binding of factors to this region of the WAF1/CIP1 promoter is not enhanced by PMA. To verify that these potential Sp1 binding sites were important for induction of transcription in response to PMA and okadaic acid, a series of CAT contructs were generated with deletions or mutations of the Sp1 consensus sequences found in the -122/-61 region of the WAF1/CIP1 promoter (diagrammed in Fig. 3and Fig. 4). These constructs were transfected into U937 cells and analyzed for basal activity and for transcriptional response to PMA and okadaic acid ( Fig. 3and Fig. 4). As mentioned above, deletion of the -122/-61 sequence, which includes several Sp1 consensus binding sites, markedly decreased both basal and induced transcription. All transcription experiments included one transfection using the -154/+16 promoter construct; the basal transcription of this construct was set at 1.0, and basal transcription of all other constructs was normalized to this value. For each construct, percentage conversion of chloramphenicol to the acetylated form in PMA or okadaic acid-treated cells was divided by percentage conversion in untreated cells to obtain values for induced transcription.


Figure 4: A consensus Sp1 binding site between bases -117 and -112 is necessary for WAF1/CIP1 promoter activation in response to PMA or okadaic acid. Mutated PCR primers were used to introduce base pair changes to the wild type WAF1/CIP1 promoter sequence, which are indicated by underlining. Consensus Sp1 binding sites are shown above the sequence. U937 cells were transfected as described in Fig. 4; the numbers represent an average of three independent transfections, and were calculated as in Fig. 4.



Deletion of sequences between -131 and -117, immediately 5` to the upstream Sp1 consensus binding site, decreased PMA induction by a small amount and decreased okadaic acid induction approximately 50%. A further deletion of sequences from -117 to -100, which eliminates the Sp1 binding sites entirely, markedly decreased basal transcription and induction in response to PMA and okadaic acid. In comparison, deletion of the downstream region between -81 and -62 knocked out okadaic acid response while having little effect on PMA response. PMA induction appears to require only the upstream element, while okadaic acid induction requires both the upstream and downstream elements.

To more precisely examine the role of the upstream Sp1 consensus site in mediating WAF1/CIP1 transcription, mutations were introduced into the -131/+16 promoter construct (Fig. 4). Mutation of three bases in the first Sp1 site decreased both basal and induced transcription by PMA and okadaic acid; the reduction in activity was approximately the same as that observed when the region was deleted. This result confirms that the Sp1 binding site is necessary for induction. Mutation of bases outside the Sp1 consensus sequence had little effect on promoter activity (Fig. 4).

To test for binding of Sp1 (or other factors) at these sites, double-stranded oligonucleotides containing WAF1/CIP1 promoter sequence from -128 to -99 or from -86 to -57 were used for gel mobility shift experiments with nuclear extracts from U937 cells (Fig. 5). Both oligonucleotides bind to a set of three proteins or protein complexes, which closely resemble the set of proteins previously observed to bind to both Sp1 sites and retinoblastoma control elements(24, 25) . These proteins are usually designated 1A, 1B, and 2, (see Fig. 5); 1A has been identified as the Sp1 gene product based on interactions with anti-Sp1 antibodies(25) , and the other bands are postulated to be Sp1-related proteins(26) . As shown in Fig. 5, preincubation of U937 nuclear extract with anti-Sp1 antibodies (21) disrupts binding of the 1A protein to both WAF1/CIP1 promoter oligonucleotides. All three proteins (1A, 1B, and 2) can also be competed off the WAF1/CIP1 -128/-99 promoter oligonucleotide with excess unlabeled -128/-99 oligonucleotide or an oligonucleotide containing the SV 40 Sp1 binding site (Promega) but not by an oligonucleotide that does not contain an Sp1 consensus sequence (see ``Materials and Methods'' for sequence), suggesting that the proteins that bind to both footprinted regions are Sp1 or Sp1-related factors.


Figure 5: Sp1 and related proteins bind to the WAF1/CIP1 promoter at the sites protected from DNase I digestion. Gel mobility shift experiments were performed using either radiolabeled -128/-99 oligonucleotide or radiolabeled -86/-57 oligonucleotide, containing the sequences from the WAF1/CIP1 promoter protected from DNase I digestion and necessary for PMA/okadaic acid induction, as shown in Fig. 3and Fig. 4. Oligonucleotides were incubated with U937 cell nuclear extract and preimmune serum or anti-Sp1 serum (first four lanes on the left). Nuclear extracts were also incubated with radiolabeled -128/-99 WAF1/CIP1 promoter oligonucleotide and with cold competitor oligonucleotides (right). The competitor oligonucleotides used are indicated above the lanes: the -128/-99 oligonucleotide itself, a WAF1/CIP1 promoter oligonucleotide containing no Sp1 sites, and an oligonucleotide containing the SV 40 Sp1 binding site. The set of proteins that bind to the Sp1 consensus sites are commonly designated 1A, 1B, and 2, as indicated on the left.



To verify that Sp1 activates transcription of the WAF1/CIP1 promoter, WAF1/CIP1 contructs were transfected into the Sp1-deficient Drosophila Schneider SL 2 cell line either in the presence or in the absence of the Sp1 expression vector pPacSp1 (27) . In the Sp1-deficient Drosophila cells, WAF1/CIP1 constructs containing sequence from -117 to +16 are highly induced in response to exogenous Sp1 expression (Fig. 6). Deletion of either the upstream or downstream Sp1 binding sites individually has a partial effect on the level of expression in response to Sp1, but deletion of both sites results in a much greater reduction in promoter activity induced by cotransfecting the Sp1 expression vector (Fig. 6). This result is similar to the pattern observed in U937 cells for response to PMA or okadaic acid, although deletion of individual sites can have a more severe effect on response to PMA or okadaic acid in the U937 cells.

Sp1 has been shown to interact with the TATA box binding protein (TBP)-associated protein TAF110 and, in collaboration with another TBP-associated protein, TAF250, activate transcription(28, 29) . If Sp1 and the complex of TBP proteins are sufficient for induction of transcription in response to PMA or okadaic acid, it is predicted that transcription of a reporter plasmid containing only multiple Sp1 binding sites and a TATA box would be stimulated by PMA or okadaic acid treatment of U937 cells. To evaluate this possibility the plasmid pGAGC6, containing six Sp1 binding sites and an adenovirus TATA box, was transfected into U937 cells, and the cells were treated with PMA or okadaic acid. Both PMA and okadaic acid induced transcription from this vector, while control vector not containing Sp1 sites was not induced (Fig. 7).


Figure 7: Transcription of a reporter plasmid containing six Sp1 binding sites is induced by PMA and okadaic acid in U937 cells. Plasmid pGAGC6, containing six Sp1 binding sites and an adenovirus TATA box upstream of a luciferase reporter gene, was transfected into U937 cells. Cells were treated with PMA or okadaic acid as in Fig. 2; cell extracts were then assayed for luciferase activity. -Fold induction is an average calculated from at least four independent transfection experiments. Vector without Sp1 sites (pGAM) was used as a control.




DISCUSSION

The association of WAF1/CIP1 expression with differentiation in many types of cultured cells suggests that p53-independent induction of WAF1/CIP1 may have a role in cell differentiation in vivo; the pattern of WAF1/CIP1 expression during mouse embryogenesis also correlates with terminal differentiation of skeletal muscle, cartilage skin, and nasal epithelium(12) . Identification of the WAF1/CIP1 promoter elements that mediate p53-independent induction of transcription should help to identify the signal transduction pathways that stimulate expression of WAF1/CIP1 during cell differentiation.

Since WAF1/CIP1 expression is stimulated by a number of agents, including platelet-derived growth factor, fibroblast growth factor, interleukin 2, tumor growth factor beta, phorbol esters, okadaic acid, retinoic acid, and vitamin D(3)(15, 30) , it is possible that a number of elements in the WAF1/CIP1 promoter function together to precisely regulate the level of WAF1/CIP1 expression. Such multiple signals might be necessary to generate sufficient WAF1/CIP1 expression for growth inhibition, since there is some evidence that the ratio of WAF1/CIP1 protein to target molecules, such as cyclin-dependent kinases, determines its effect(31) . This idea is supported by the fact that the WAF1/CIP1 promoter element responsible for induction in response to serum in fibroblasts appears to be located between -1,817 and -4699 bases upstream of the transcription start site(14) , while induction in response to tumor growth factor beta in SW480 cells is mediated by elements between -61 base pairs and -1.1 kb upstream(17) . These results suggest that there are at least two p53-independent pathways for induction of WAF1/CIP1 transcription.

We now report that induction of the WAF1/CIP1 promoter in U937 leukemic cells by PMA and okadaic acid involves Sp1 binding sites located approximately 115 and 80 bases upstream of the transcription start site; loss of these sites results in lack of response to PMA and okadaic acid as well as loss of WAF1/CIP1 promoter induction in response to exogenous Sp1 in the Sp1-deficient Drosophila Schneider SL2 cell line. The upstream(-115) Sp1 site is vital for full response of the promoter to PMA in U937 cells, but all potential Sp1 binding sites between -122 and -61 must be deleted to abolish induction by exogenous Sp1 in the SL2 cells. The requirements for utilization of an Sp1 binding site in U937 cells may be more stringent due to the presence of Sp1-related proteins (26) or Sp1-inhibitory proteins (25) that are not present in the Drosophila SL2 cells. The fact that the reporter plasmid pGAGC6 (containing multiple Sp1 binding sites upstream of a TATA box) is induced by PMA and okadaic acid in U937 cells suggests that Sp1 may be sufficient for increased transcription of the WAF1/CIP1 promoter in response to PMA or okadaic acid treatment.

The Sp1 transcription factor is found in glycosylated and phosphorylated forms, but little is known about how these modifications affect function(32) . Interactions between Sp1 and the retinoblastoma protein have also been reported; a 20-kDa inhibitor of Sp1 (Sp1-I) was identified that also bound to Rb, and it was proposed that Rb binds and inactivates Sp1-I, leading to transcriptional activation by Sp1(24) . A 74-kDa protein that binds to the transactivation domain of Sp1 and inhibits Sp1-mediated transactivation has also been identified(33) . These reports suggest that there are multiple inhibitors of Sp1, which could inhibit interaction of Sp1 with the transcription factor IID complex(28) . It is also possible that proteins of the transcription factor IID complex are modified in response to PMA, or that the composition of the transcription factor IID complex is altered by gain or loss of TBP-associated factors (TAFs). Recent studies have shown that some TAFs may serve as coactivators to mediate transcriptional regulation(34) .

Signal transduction pathways that activate or inactivate such Sp1 inhibitors may serve to regulate Sp1 transcriptional activation and may therefore regulate WAF1/CIP1 and other genes involved in cell differentiation. A number of signal transduction pathways composed of kinase cascades have been described in the literature(35) . However, a variety of signals that activate either the mitogen-activated protein kinase pathway, the stress-activated protein kinase pathway, or the p38 kinase pathway fail to induce WAF1/CIP1 transcription in U937 cells. These signals include UV irradiation, activated mitogen-activated protein kinase kinase, and osmotic shock.^2 This suggests that the signals that induce WAF1/CIP1 transcription via Sp1 may be part of an as yet unidentified pathway.


FOOTNOTES

*
This work was supported by Grant CA42533 and American Cancer Society (ACS) Grant DHP 83 (to A. S. K.), Grant DK 43652 (to J. E. K.), and an institutional ACS grant and Milheim Foundation grant (to J. B.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
To whom correspondence should be addressed. Tel.: 205-934-4436; Fax: 205-975-6911.

(^1)
The abbreviations used are: PMA, phorbol myristate acetate; kb, kilobase(s); CAT, chloramphenicol acetyltransferase; TBP, TATA box binding protein; TAF, TBP-associated factor; OK, okadaic acid.

(^2)
J. Biggs, unpublished observations.


ACKNOWLEDGEMENTS

We thank Bert Vogelstein and Todd Waldman for clones of the WAF1/CIP1 promoter sequence.


REFERENCES

  1. Harris, P., and Ralph, P. (1985) J. Leukocyte Biol. 37, 401-422
  2. Kraft, A. S., Barker, V. V., and May, W. S. (1987) Oncogene 1, 111-118 [Medline] [Order article via Infotrieve]
  3. Yen, A., Forbes, M., DeGala, G., and Fishbaugh, J. (1987) Cancer Res. 47, 129-134 [Abstract]
  4. Hass, R., Bartels, H., Topley, N., Hadam, M., Kohler, L., Goppelt-Strube, M., and Resch, K. (1989) Eur. J. Cell Biol. 48, 282-293 [Medline] [Order article via Infotrieve]
  5. Aduyah, S. E., Unlap, T. M., Franklin, C. C., and Kraft, A. S. (1991) J. Cell. Physiol. 151, 415-426
  6. Ishida, Y., Furukawa, Y., DeCaprio, J. A., Saito, M., and Griffin, J. D. (1992) J. Cell. Physiol. 150, 484-492 [Medline] [Order article via Infotrieve]
  7. El Diery, W., Tokino, T., Velculescu, V. E., Levy, D. B., Parsons, R., Trent, J. M., Lin, D., Mercer, W. E., Kinzler, K. W., and Vogelstein, B. (1994) Cell 75, 817-825
  8. Harper, J. W., Adami, G. R., Wei, N., Keyomarsi, K., and Elledge, S. J. (1993) Cell 75, 805-816 [Medline] [Order article via Infotrieve]
  9. Dulic, V., Kaufmann, W. K., Wilson, S. J., Tlsty, T. D., Lees, E., Harper, J. W., Elledge, S. J., and Reed, S. (1994) Cell 76, 1013-1023 [Medline] [Order article via Infotrieve]
  10. El Deary, W. S., Harper, J. W., O'Connor, P. M., Velculescu, V. E., Canman, C. E., Jackman, J., Pietenpol, J. A., Burrell, M., Hill, D. E., Wang, Y., Wiman, K. G., Mercer, W. E., Kastan, M. B., Kohn, K. W., Elledge, S. J., Kinzler, K. W., and Vogelstein, B. (1994) Cancer Res. 54, 1169-1174 [Abstract]
  11. Halevy, O., Novitch, B. G., Spicer, D. B., Skapek, S. X., Rhee, J., Hannon, G. J., Beach, D., and Lasser, A. B. (1995) Science 267, 1018-1021 [Medline] [Order article via Infotrieve]
  12. Parker, S. B., Eichele, G., Zhang, P., Rawls, A., Sands, A. T., Bradley, A., Olsen, E. N., Harper, J. W., and Elledge, S. J. (1995) Science 267, 1024-1027 [Medline] [Order article via Infotrieve]
  13. Jiang, H., Lin, J., Su, Z., Collart, F. R., Huberman, E., and Fisher, P. B. (1994) Oncogene 9, 3397-3406 [Medline] [Order article via Infotrieve]
  14. Macleod, K. F., Sherry, N., Hannon, G., Beach, D., Tokino, T., Kinzler, K., Vogelstein, B., and Jacks, T. (1995) Genes & Dev. 9, 935-944
  15. Michieli, P., Chedid, M., Lin, D., Pierce, J. H., Mercer, W. E., and Givol, D. (1994) Cancer Res. 54, 3391-3395 [Abstract]
  16. Elbendary, A., Berchuck, A., Davis, P., Havrilesky, L., Bast, R. C., Iglehart, J. D., and Marks, J. R. (1994) Cell Growth & Differ. 5, 1301-1307
  17. Datto, M. B., Li, Y., Panus, J., Howe, D., Xiong, Y., and Wang, X. F. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 5545-5549 [Abstract]
  18. Fridovich-Keil, J. L., Gudas, J. M., Bryan, I. B., and Pardee, A. B. (1991) BioTechniques 11, 572-578 [Medline] [Order article via Infotrieve]
  19. William, F., Wagner, F., Karin, M., and Kraft, A. S. (1990) J. Biol. Chem. 265, 18166-18171 [Abstract/Free Full Text]
  20. Andrews, N. C., and Faller, D. V. (1991) Nucleic Acids Res. 19, 2499 [Medline] [Order article via Infotrieve]
  21. Shin, T. H., Paterson, A. J., Grant, J., II, Meluch, A. A., and Kudlow, J. E. (1992) Mol. Cell. Biol. 12, 3998-4006 [Abstract]
  22. Cao, X., Mahendran, R., Guy, G. R., and Tan, Y. H. (1993) J. Biol. Chem. 268, 16949-16957 [Abstract/Free Full Text]
  23. Lin, Y., Brown, K., and Siebenlist, U. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 552-556 [Abstract]
  24. Udvadia, A. J., Rogers, K. T., Higgins, P. D. R., Murata, Y., Martin, K. H., Humphery, P. A., and Horowitz, J. M. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 3265-3269 [Abstract]
  25. Chen, U. I., Nishinaka, T., Kwan, K., Kitabayashi, I., Yokoyama, K., Fu, Y. F., Grunwald, S., and Chiu, R. (1994) Mol. Cell. Biol. 14, 4380-4389 [Abstract]
  26. Kingsley, C., and Winoto, A. (1992) Mol. Cell. Biol. 12, 4251-4261 [Abstract]
  27. Courey, A. J., and Tjian, R. (1988) Cell 55, 887-898 [Medline] [Order article via Infotrieve]
  28. Gill, G., Pascal, E., Tseng, Z. H., and Tjian, R. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 192-196 [Abstract]
  29. Weinzierl, R. O. J., Dynlacht, B. D., and Tjian, R. (1993) Nature 362, 511-517 [CrossRef][Medline] [Order article via Infotrieve]
  30. Nourse, J., Firpo, E., Flanagan, W. M., Coats, S., Polyak, K., Lee, M. H., Massague, J., Crabtree, G. R., and Roberts, J. M. (1994) Nature 372, 570-572 [Medline] [Order article via Infotrieve]
  31. Zhang, H., Hannon, J. G., and Beach, D. (1994) Genes & Dev. 8, 1750-1758
  32. Jackson, S. P., MacDonald, J. J., Lees-Miller, S., and Tjian, R. (1990) Cell 63, 155-165 [Medline] [Order article via Infotrieve]
  33. Murata, Y., Kim, H. G., Rogers, K. T., Udvadia, A., and Horowitz, J. M. (1994) J Biol. Chem. 269, 20674-20681 [Abstract/Free Full Text]
  34. Tjian, R., and Maniatis, T. (1994) Cell 77, 5-8 [Medline] [Order article via Infotrieve]
  35. Levin, D. E., and Errede, B. (1993) J. NIH Res. 5, 49-52

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