©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
Selective Activation of T Cell Kinase p56 by Herpesvirus saimiri Protein Tip (*)

(Received for publication, July 13, 1995; and in revised form, November 3, 1995)

Nicole Wiese Alexander Y. Tsygankov (1) Ulricke Klauenberg Joseph B. Bolen (2) Bernhard Fleischer Barbara M. Bröker (§)

From the  (1)Bernhard-Nocht-Institut für Tropenmedizin, Bernhard-Nocht-Strasse 74, 20359 Hamburg, Federal Republic of Germany, the Department of Microbiology and Immunology, Temple University Medical School, Philadelphia, Pennsylvania 19140, and the (2)DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

Infection with Herpesvirus saimiri, a T lymphotropic virus of non-human primates, immortalizes human T cells in vitro. The cells show a mature activated phenotype and retain their antigen specificity. We have previously shown that in H. saimiri transformed cells a viral gene product termed tyrosine kinase interacting protein (Tip) associates with the T cell-specific tyrosine kinase p56 and becomes phosphorylated by the enzyme on tyrosine residues. Here we show that p56 is activated by recombinant and native Tip in cell-free systems. A dramatic increase of Lck activity was also observed in T cell lines transfected with Tip. p60 and p53/56, the other Src-related kinases expressed in H. saimiri transformed T cells, did not phosphorylate Tip, and they were not activated by the protein. The selective activation of p56 by Tip could contribute to the transformed phenotype of H. saimiri infected cells, and it might explain the T cell selectivity of the transformation event.


INTRODUCTION

Certain strains of Herpesvirus saimiri readily transform human T cells to continuous growth in cell culture(1) . Remarkably, the antigen-specific response of the immortalized T cells as well as their mature and activated phenotype are very stable. The T cell receptor-CD3 complex, the CD4 or CD8 coreceptors, CD2, and the IL-2 (^1)receptors remain present and functionally competent over many months in culture(2, 3, 4, 5, 6) , suggesting that the signaling apparatus of mature T cells may be required for the viral transformation. The viral genome harbors approximately 75 open reading frames. Many have been tested for transcription in transformed human cells but, so far, only ORF1 and ORF2 (7) encoded on a single bicistronic mRNA have been found in the permanently growing cells. We have previously shown, that the gene product of ORF1, tyrosine kinase interacting protein (Tip) is expressed in transformed cells. Tip associates with the protein tyrosine kinase p56 and can be phosphorylated by this enzyme in vitro(8) . p56is a non-receptor tyrosine kinase of the Src family which is selectively expressed in thymocytes and mature T cells. The kinase tightly associates with the coreceptor molecules CD4 or CD8 and becomes activated after cross-linking of these receptors on the cell surface. One of the substrates of p56 is the -chain of the T cell receptor, and T cells deficient in p56 fail to respond to T cell receptor mediated signals. This attributes a crucial role to the enzyme in the antigen-specific response of T cells(9, 10) . The ORF2-encoded saimiri transformation-associated protein (StpC) is also expressed in H. saimiri transformed human T cells and might complement the action of Tip. StpC is a strong oncoprotein in rodent fibroblasts and epithelial cells of transgenic mice, but not in T cells(11, 12) . The mechanism of oncogenesis by StpC is not known. Transformation of T cells probably requires the action of Tip on the T cell-specific kinase p56in addition to StpC. Here we further characterize this interaction and show that Tip selectively interacts with p56, but not with the other Src-related kinases p60 or p53/56 which are expressed in human T cells immortalized by H. saimiri. Furthermore, this interaction leads to a dramatic activation of the enzyme. This may explain why many human cell types can be infected by H. saimiri but only T cells are transformed to permanent growth.


EXPERIMENTAL PROCEDURES

Cells and Cell Culture

The human CD4+ T cell clones 61/39, 61/48, 68/4, and 68/5 as well as their H. saimiri immortalized derivative T cell lines 39-HVS, 48-HVS, 68/4-HVS, and 68/5-HVS have been described previously(2, 8) . CB15 is a CD4+ T cell line derived from cord blood(1) , 3C a CD8+ T cell line derived from peripheral blood by infection with H. saimiri. Nontransformed T cell clones were cultured in complete medium (CM, RPMI 1640, 4 mML-glutamine, 50 µg/ml gentamicin, and 10% screened fetal bovine serum) supplemented with 100 units/ml recombinant human IL-2 (Eurocetus, Amsterdam, Netherlands) at 37 °C, 5% CO(2) in humidified atmosphere. They were restimulated every 14 days with 0.5 µg/ml phytohemagglutinin in the presence of irradiated peripheral blood mononuclear cells. After transformation with H. saimiri, human T cell lines were kept in 50% CG medium (Vitromex, Vilshofen, Germany) and 50% CM in the presence of 100 units/ml IL-2. Jurkat cells were derived from the original culture(13) . Raji and BJAB are Burkitt's Lymphoma lines. BW, a murine thymoma line stably transfected with the murine CD3--chain and the human -chain(14) , was a gift of B. Malissen. Raji, BJAB, and BW were grown in complete medium.

Antisera and Monoclonal Antibodies

Antisera to the unique regions of Src-related kinases were raised by immunizing rabbits with fusion proteins containing the unique regions of murine p56, p60, p53/56, and p62 and glutathione S-transferase. Antisera to the Src-kinases p55, p59, and p58 were generated by immunization of rabbits with synthetic peptides corresponding to the unique regions (15) . A monoclonal antibody reactive with p60 has been described(15) . The anti-phosphotyrosine antibody PY-20 was purchased from Transduction Laboratories (Lexington, KY), a rabbit antiserum to phosphotyrosine was obtained from UBI (Lake Placid, NY). Antisera against Tip were a gift of H. Fickenscher, Erlangen, Germany, and have been described(8) . The monoclonal anti-Tip antibody BNI2 was generated from spleen cells of Balb/c mice immunized with a beta-Gal-Tip fusion protein which has been described(8) . BNI2 reacts with beta-Gal-Tip in enzyme-linked immunosorbent assay and immunoblot sytems, and it stains a 40-kDa band in cell lines transfected with full-length Tip (see below) but not in nontransfected cells. Rabbit anti-mouse polyclonal sera were purchased from Dianova (Hamburg, Germany).

Immunoprecipitations

Cells (1.5 times 10^7/ml in serum-free medium) were lysed in TNE buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 2 mM EDTA, and 1% Nonidet P-40) supplemented with 1 mM sodium orthovanadate (Na(3)VO(4)), 5 mM NaF, and 10 µg/ml each of aprotinin and leupeptin (Sigma) for 20 min on ice. Lysates were cleared at 14,000 times g for 5 min, and the protein concentration in the supernatants was determined. 5 µl of antiserum/mg of protein were added for at least 1 h at 4 °C to precipitate Src-related kinases. This was followed by incubation with 50 µl of a 10% (v/v) suspension of Staphylococcus aureus particles (Pansorbin, Calbiochem). In the case of murine antibodies, the S. aureus particles were preincubated with 50 µg/ml rabbit anti-mouse antibodies and washed. The immunoprecipitates were washed five times in TNE buffer. For reprecipitation after the in vitro phosphotransferase reaction (see below), the phosphotransferase reaction was stopped by boiling samples in 1% SDS, 10 mM Tris, and 1 mM sodium orthovanadate. This dissociated the immune complexes. The detergent was then diluted at least 1/10 with 1% Triton, 10 mM Tris, 50 mM NaCl, 5 mM EDTA and the solution was precleared from the remaining antibodies by incubation with 50 µl of S. aureus suspension followed by centrifugation. The supernatants were incubated with the second antibody for 1 h at 4 °C and precipitated with S. aureus as described above.

In Vitro Phosphotransferase Assay

Immunoprecipitates were washed once in kinase buffer (20 mm MOPS, pH 7.0, and 5 mM MgCl(2)). The pellets were then incubated for 5 min at room temperature in 25 µl of kinase assay mixture containing 1 µM ATP (Boehringer Mannheim), 10 µCi of [-P]ATP (Amersham) and 2 µg of rabbit muscle enolase (Sigma) in kinase buffer. The phosphotransferase reaction was stopped with sample buffer (62.5 mM Tris, pH 6.8, 2% SDS, 10% glycerol, and 5% beta-mercaptoethanol). Samples were then incubated for 30 min at room temperature and microfuged. Supernatants were boiled for 5 min before separation by 8% SDS-PAGE. Gels were dried and autoradiographed. For the experiments described in Fig. 4D, 0.1 µg of recombinant p56 was incubated in 50 µl of kinase buffer in the presence of different amounts of beta-Gal or beta-Gal-Tip as indicated. The kinase reaction was started by the addition of 25 µCi of [-P]ATP and carried out for 5 min at room temperature. It was stopped with sample buffer, and samples were separated by 8% SDS-PAGE. Gels were dried and exposed to Kodak XAR5 film. Quantitative analysis was performed on a PhosphorImager (Molecular Dynamics).


Figure 4: Enzymatic activation of p56 by recombinant and native Tip. p56 was precipitated from Jurkat cells (200 µg of protein/sample), and an immune complex kinase assay was carried out in the presence of 2 µg of enolase as exogenous substrate. Increasing amounts of beta-Gal-Tip (A), beta-Gal (B), and Lck-depleted BW-Tip lysate as a source of native Tip (C) were added. The Lck-depleted lysate of untransfected BW cells served as control in C. In D, increasing amounts of beta-Gal-Tip were added to 0.5 µg of recombinant p56, and a phosphotransferase reaction was performed. beta-Gal was used as control. The proteins were separated by 8% SDS-PAGE, and the gels were dried and exposed to x-ray film. The positions of Lck, Tip, beta-Gal-Tip, and beta-Gal are on the right, molecular mass standards are on the left.



Immunoblots

For immunoblotting, cell lysates or immunoprecipitates were separated by 8% or 12% SDS-PAGE and transferred to nitrocellulose membranes. Blots were incubated for 1 h at room temperature in blocking buffer (phosphate-buffered saline, pH 7.4, 5% bovine serum albumin, and 0.5% Tween 20) followed by incubation with antisera diluted in blocking buffer. After thorough washing in phosphate-buffered saline containing 0.5% Tween 20, blots were incubated with 1 µCi/ml I-protein A (Amersham) in blocking buffer for at least 1 h, washed again, dried, and exposed to Kodak XAR5 film. Quantitative analysis was performed on a PhosphorImager (Molecular Dynamics). For the experiment described in Fig. 6B, the blot was incubated with the mouse monoclonal antibody BNI2. Bands were visualized by incubation with goat anti-mouse Ig coupled to horseradish peroxidase followed by enhanced chemiluminescence (Amersham) according to the manufacturer's instructions.


Figure 6: Tyrosine phosphorylation analysis. A, transformation by H. saimiri increases the cellular content of phosphotyrosine. Phosphotyrosine-containing proteins were precipitated from 2 mg of protein of T cell lines transformed by H. saimiri, from their nontransformed parental clones and from phytohemagglutinin-stimulated T cell blasts using the monoclonal antibody PY-20. For control, only PY-20 but no cell lysate was added. After separation by 12% SDS-PAGE and transfer to a nitrocellulose membrane, bands containing phosphotyrosine were revealed with a polyclonal antiserum and I-protein A followed by autoradiography. B, Tip is phosphorylated on tyrosine residues in vivo. Phosphotyrosine-containing proteins were precipitated from cell lysates (200 µg of protein) as described above (lanes 1-3) and separated by 8% SDS-PAGE. 50 µg of whole cell lysate from the same cell lines was loaded in lanes 4-6 of the same gel. Proteins were transferred to nitrocellulose, and the blot was probed with the anti-Tip monoclonal antibody BNI2. Bands were visualized by enhanced chemiluminescence. Tip expression in H. saimiri transformed cells (e.g. 39-HVS) regularly escaped detection by immunoblotting. The positions of Tip and the Ig heavy and light chains are shown on the right, the molecular mass markers are on the left.



Recombinant Proteins and Cell Transfection

A fusion protein of p56 and glutathione S-transferase (GST-Lck) was produced in a baculovirus expression system and purified by affinity chromatography on glutathione-Sepharose columns(16) . For the experiment described in Fig. 4D, GST-Lck was cleaved with thrombin (ICN, Meckenheim, Germany), and uncleaved GST-Lck as well as GST were precipitated with glutathione-Agarose (Sigma). The supernatant contained p56.

A fusion protein of amino-terminally truncated Tip and beta-galactosidase as well as beta-galactosidase alone were gifts of B. Biesinger, Erlangen, Germany, and have been described(8) . Tip-encoding sequences on the plasmid p488PX (gift of B. Biesinger(7) , EMBL accession number M55264) were amplified by polymerase chain reaction with the following primers: forward, CGCGGGCTCGAGATGGCAAATGAAGGAGAAGAA; reverse, CCGGCCGCGGCCGCTTAACTTTTCATTCCTATATG. This inserted a XhoI restriction site 5` and a NotI restriction site 3` of the coding regions which were used to clone the gene into the eukaryotic expression vector BCMGSNeo (gift of H. Karasuyama(17) ). Plasmids were transfected into BW cells by electroporation. One day after transfection, 1 mg/ml G418 was added to the cultures for selection. Growing cell lines were characterized by immunoprecipitation of p56 followed by phosphotransferase assay and reprecipitation with the anti-Tip serum. A cell line expressing large amounts of Tip was cloned to obtain BW-Tip.


RESULTS

p56 but Not p60 or p53/56 Phosphorylate Tip in Vitro

As we have shown previously, p56 associates with the herpesviral gene product Tip in transformed T cells, and it phosphorylates the protein in vitro. We first determined whether Tip also interacts with other Src-related kinases which are expressed in the transformed T cells. For this we screened 5 human CD4+ T cell lines and 3 CD8+ T cell lines transformed by H. saimiri for the expression of Src-related protein tyrosine kinases (Lck, Fyn, Yes, Src, Lyn, Blk, Hck, and Fgr) with immunoprecipitation followed by in vitro phosphotransferase assay. They were compared with 5 nontransformed human T cell clones. As expected, in all T cell clones, basal activity of p56 and p60 was measured. All transformed T cell lines additionally exhibited significant enzymatic activity of p53/56, which is not commonly found in T cells. Fig. 1shows the gain of p53/56 activity in the human T cell clone 39-HVS after infection with H. saimiri. We then looked for co-immunoprecipitation of Tip with p60 or p53/56 in transformed T cells. This was never observed (data not shown). However, p60 and p53/56 are present in the T cells at much lower amounts than p56 (Fig. 1), and the level of Tip expression in transformed T cells is also very low. To increase the sensitivity of our assay, we precipitated p60 and p53/56 from the B cell lines Raji or BJAB where it is much more abundant. Furthermore, this excluded contamination of the immune complexes with p56. An in vitro phosphotransferase reaction was performed with recombinant Tip fused to beta-galactosidase. As expected, Tip was readily bound and phosphorylated by p56 precipitated from nontransformed human T cells or Jurkat cells. Again, p60 and p53/56 were unable to phosphorylate Tip (Fig. 2). To generate a source of native Tip, we transfected the mouse T cell line BW. BW-Tip expresses about 1 µg of Tip/mg of cell protein as determined by comparison with beta-Gal-Tip on immunoblots (Fig. 3A and data not shown), but about 200-fold less p56 than the human T cell line Jurkat which is representative of the other T cell lines used in our experiments (Fig. 3B). Immune complex phosphotransferase assays performed with p56 and p60 immunoprecipitated directly from BW-Tip again showed selective association and phosphorylation of Tip by p56 (Fig. 3A and data not shown). Finally, we used recombinant GST-Lck, GST-Fyn, and GST-Lyn as well as Lck, Fyn, and Lyn precipitated from noninfected human T cells and B cells as above to co-immunoprecipitate Tip from BW-Tip lysates which had been depleted of endogenous Lck. The results confirmed that p60 and p53/56 are not able to bind and phosphorylate H. saimiri Tip (data not shown).


Figure 1: Expression of p53/56 in addition to p56 and p60 in human T cells after infection with H. saimiri. Lysates were prepared from the nontransformed T cell clone 61/39 (A) and from the same T cell clone 6 months after transformation by H. saimiri (39-HVS, B). Src-related kinases were immunoprecipitated from 500 µg of total cell protein/sample except for p56 where 100 µg of total cell protein was used. S. aureus particles without anti-kinase serum served as control. An in vitro kinase assay was performed, and the resulting phosphoproteins were separated by 8% SDS-PAGE. The two gels were electrophoresed in parallel, dried, and exposed to x-ray film for 2 h to reveal autophosphorylation. Molecular mass standards are on the right.




Figure 2: p56 but not p60 or p53/56 specifically phosphorylates recombinant Tip in vitro. p56 was immunoprecipitated from Jurkat cell lysates (600 µg/sample), p60 from Lyn-depleted lysates of BJAB cells (300 µg/sample), and p53/56 from lysates of Raji cells (900 µg/sample). S. aureus particles without anti-kinase antibody served as control. An immune complex kinase assay was performed without addition or in the presence of 1 µg of either the beta-Gal-Tip fusion protein or of unfused beta-Gal as indicated. Molecular mass standards are shown on the left, and the positions of beta-Gal-Tip or beta-Gal are on the right.




Figure 3: Characterization of the murine T cell line BW transfected with p40. A, association of p40 with Lck. p56 was precipitated from 100 µg of protein of the transfected T cell line BW-Tip (+) and from 3000 µg of protein of its mock-transfected counterpart BW-neo(-). An immune complex kinase assay was performed and proteins were separated by 8% SDS-PAGE. The gel was dried and autoradiographed. Different amounts of total cellular protein were used for immunoprecipitation in order to achieve comparable levels of Lck autophosphorylation (lanes 1 and 2). This was necessary in order to demonstrate convincingly the absence of a 40-kDa band in the BW-neo cells. The 40-kDa band in Lck immunoprecipitates from BW-Tip was then identified by dissociation of the immune complexes and reprecipitation of Tip with a specific antiserum (lanes 3 and 4). Proteins in these precipitates were separated on the same gel which was dried and autoradiographed. B, expression of p56. Lck was immunoprecipitated from different protein amounts of BW-Tip and of Jurkat cells as indicated and subjected to 8% SDS-PAGE. After transfer to a nitrocellulose membrane, the Lck protein amount was visualized by probing with a rabbit serum directed against Lck followed by I-protein A. The immunoblot was then autoradiographed. PhosphorImager analysis revealed that BW-Tip contained 220-fold less Lck than Jurkat cells. The positions of Lck and Tip are indicated on the left, molecular mass standards are on the right.



Increase of p56 Enzymatic Activity by Tip

p56 was immunoprecipitated from Jurkat cells, and the enzymatic activity was determined by a phosphotransferase assay in the presence of rabbit muscle enolase as exogenous substrate. Addition of increasing concentrations of beta-Gal-Tip fusion protein augmented the enzymatic activity of Lck by a factor of 3.4 while beta-Gal alone showed a negative effect if any (Fig. 4, A and B). Similarly, lysates of BW-Tip depleted of endogenous p56 enhanced the phosphorylation of enolase by Lck about 6-fold in this system, while lysates of the parent cell line did not affect Lck activity (Fig. 4C). Recombinant Lck could be used instead of the Lck immune complexes in the latter experiments with comparable results (data not shown). Neither beta-Gal-Tip nor native Tip precipitated from Lck-free cell lysates ever showed kinase activity in these systems (data not shown). Roughly equal amounts of both recombinant (Fig. 4A) and cell-derived (Fig. 4C) Tip were needed to activate Lck; the amount of p40 present in 1 mg of BW-Tip cell protein (1 µg) is equivalent to 2 µg of beta-Gal-Tip (M(r) = 90,000). At very high Tip concentrations, phosphorylation of enolase decreased again due to substrate competition (data not shown). This precludes accurate measurement of the maximal activation of Lck by Tip. The factor of about 3-6 is, therefore, a conservative estimate of the enzymatic activation of p56 by Tip. To exclude that activation of p56 by viral Tip is mediated by other T cell-derived molecules which might have coprecipitated with either Lck or Tip in the experiments shown in Fig. 4, A-C, Lck was cleaved from GST-Lck by thrombin and incubated with beta-Gal-Tip. Again, beta-Gal-Tip but not the fusion partner beta-Gal enhanced the enzymatic activity of this recombinant p56 preparation as shown by an increase of Lck autophosphorylation (Fig. 4D). Re-evaluation of an effect of Tip on p60 or p53/56 in the presence of enolase never showed activation of these enzymes (data not shown).

Enzymatic Activity of Lck is Increased in T Cells Transfected with Tip

Measurement of the specific activity of Lck in BW and BW-Tip showed a dramatic increase in the Tip transfected cell line (Fig. 5A). BW cells express extremely low quantities of Lck (Fig. 3B) precluding measurement of its activity in untransfected cells and necessitating precipitation from large protein amounts prior to detection on immunoblots (Fig. 5B). In contrast, specific activation of Lck could not be demonstrated reproducibly in H. saimiri infected T cells. Tip is expressed in very low amounts in these cells and can be detected only by the sensitive phosphotransferase assay but not on immunoblots. Any effect of Tip on Lck, which is very abundant in these cells, would be masked by the background activity of Lck which is not in complex with Tip.


Figure 5: Expression of Tip increases the enzymatic activity p56 in the murine T cell line BW. p56 was precipitated from different amounts of cell lysate from the Tip-transfected T cell line BW-Tip and from its mock-transfected counterpart BW-neo. For determination of enzymatic activity, immunoprecipitates were subjected to an immune complex kinase assay, and the resulting proteins were separated by 8% SDS-PAGE (A). For measurement of protein abundance, immunoprecipitates were separated on 8% SDS-PAGE, transferred to nitrocellulose membranes, and probed with serum directed against Lck followed by I-protein A (B). The dried gel (A) and the immunoblot (B) were then autoradiographed. Controls without precipitating antibody are boxed. Note the large amounts of cell lysate necessary to visualize Lck from these murine cells on immunoblots. The specific activity of Lck in BW-Tip is, therefore, much higher than what is usually observed in T cells. The positions of Lck, enolase, and Tip are shown on the right, molecular mass standards are on the left.



Tyrosine Phosphorylation in T Cells Expressing Tip

Since an increase of specific kinase activity of p56 could not be measured in H. saimiri infected cells, we probed their basal phosphotyrosine levels as a readout for kinase activity that might not be masked by excess of inactive enzyme. Expectedly, the amount of proteins phosphorylated on tyrosine in our T cells was generally low in the absence of further stimulation. However, an increase in the level of phosphotyrosine in three bands with molecular masses of 70, 55, and 30 kDa by an average factor of 2.1, 5.3, and 2.2, respectively, were observed in T cell lines transformed by H. saimiri as compared with their nontransformed parental clones or with phytohemagglutinin-blasts (Fig. 6A). The low levels of Tip expression in T cells transformed by H. saimiri precluded detection on immunoblots (Fig. 6, A and B). Therefore, we utilized the BW-Tip cell line to demonstrate phosphorylation on tyrosine residues of Tip in vivo. After immunoprecipitation with anti-phosphotyrosine antibodies, Tip was readily demonstrated on immunoblots from BW-Tip but not from the vector control BW-neo (Fig. 6B). However, the interaction of Tip with Lck was not blocked by excess of phosphotyrosine (data not shown).


DISCUSSION

Previously we have suggested that two factors cooperate in the transformation of human T cells by H. saimiri with StpC acting as the basic oncoprotein complemented by the T cell-specific action of Tip(8) . The results of our analysis of the Tip/Lck interaction presented here lend support to our hypothesis. Tip binds to and directly activates the Src-related kinase p56, which is expressed in large amounts in T cells and thymocytes. Because of substrate competition at high concentrations of Tip, the maximal enzymatic activation of Lck could not be determined. The factor of 3-6 observed in our system is, therefore, a conservative estimate. Besides Lck, which is by far the most abundant Src-related kinase in human T cells, we observed significant activities of p60 and, unexpectedly, also of p53/56 in transformed T cells. However, neither p60 nor p53/56 was affected by Tip.

Expression of p53/56 in T cells is unusual. It has been observed previously only after infection of T cells by the retrovirus HTLV-I(18) . This could be partially explained by trans-activation of the Lyn promoter by the HTLV-I encoded transcription factor p40. However, p40 is not related to the H. saimiri encoded p40(19) . Furthermore, the appearance of Lyn in HTLV-I-infected T cells was accompanied by a gradual loss of Lck activity, which correlated with dedifferentiation of these cells to IL-2-independent growth (18) . (^2)Neither decrease of Lck abundance and activity nor IL-2 independence was observed in human T cells transformed to permanent growth by H. saimiri. The mechanisms of the aberrant activation of Lyn in these cells are not known at present.

What might be the consequences of the Tip-mediated activation of Lck, an enzyme which plays a pivotal role in T cell signaling? It is well documented that p56 which is constitutively active by virtue of mutation or deletion of its regulatory tyrosine 505 acts as an oncoprotein in fibroblasts. In these cells, wild-type p56 was not effective even when overexpressed(20, 21, 22) . In thymocytes of p56 transgenic mice, increased expression of both wild-type and active mutants of Lck induced the formation of thymomas(23) . Therefore, activation of p56 by Tip may contribute to the transformed phenotype of H. saimiri infected T cells. On the other hand, transfection of Tip into fibroblasts did not result in transformation(11) . However, fibroblasts do not express Lck, and our data show that enzymatic activation by Tip is highly selective for this Src family member. In agreement with this, no kinase activity was associated with Tip in transfected murine fibroblasts. (^3)

Transfection with constitutively active p56 resulted in increased responsiveness to T cell receptor-mediated signals in a murine T cell hybridoma, a model for mature T cells(24) . Enhanced tyrosine phosphorylation in response to TcR triggering in T cells transformed by H. saimiri has been documented earlier(3) . To investigate whether this could be the result of Lck activation by Tip, we first attempted to demonstrate an increase of the specific enzymatic activity of Lck in T cells transformed by the virus. While a dramatic increase of p56 activity was readily observed in T cells overexpressing Tip after transfection, we could not clearly show it in the T cell lines transformed by wild-type virus. This may be due to the very low level of Tip expression in these cells. Activation of a small fraction of Lck by the associated Tip would then be masked by the excess of nonactivated enzyme. However, the small fraction of Tip-activated Lck might be non-randomly distributed in the cells and still have a significant effect on the phosphorylation of selected substrates. We have, therefore, tested the basal level of tyrosine phosphorylation in T cells without any further stimulation. This most clearly reflects the influence of H. saimiri. Three bands with molecular masses of around 70, 55, and 30 kDa were more strongly phosphorylated in the virally transformed T cells. The prominent 55-kDa band, which faintly also appears in the nontransformed T cells, very likely corresponds to p56, which is by far the most abundant and most active Src family kinase in all our T cell lines. Lyn is much less active than Lck in H. saimiri transformed T cells, and it is not expressed in the nontransformed parental cell lines. Increased phosphorylation of p56 is probably a direct effect of its activation by Tip. However, it cannot be excluded that p56 or one of the other prominent bands on the phosphotyrosine immunoblot might also be a substrate of p53/56. Other nonidentified kinases or phosphatases may also play a role.

One of the most striking changes in the behavior of human T cells transformed by H. saimiri is their hyper-reactivity to ligation of CD2. Because the ligand for CD2, LFA-3, is also expressed on these cells, cell contact leads to ligation of CD2, which results in autostimulation, IL-2 production, and autocrine growth(6) . Blockade of the CD2/LFA-3 interaction can halt the growth of H. saimiri transformed T cells, so that this autocrine loop appears to be essential for the transformed phenotype (6) . (^4)p56 has been found in complex with CD2, and the enzyme becomes activated after CD2 cross-linking(25, 26) . Therefore, activation of p56 by Tip could enhance the T cell responses to CD2. However, the dissection of CD2-mediated signaling in H. saimiri transformed T cells requires further investigation.

The mechanism by which Tip activates p56 is not understood. It is likely that the hydrophobic stretch at the carboxyl terminus of Tip inserts into the cell membrane and brings the molecule into proximity of Lck. In fact, the Tip homologue of a related H. saimiri strain is located in the outer cell membrane(27) . The Tip sequence contains a 10-amino acid stretch with strong similarity to a sequence in the carboxyl terminus of Src family kinases as well as a type II SH3-domain binding motif(8) . These are necessary and sufficient for efficient binding of Tip to Lck(28) . Analysis of the crystal structure of the regulatory domains of Lck revealed dimerization of the SH2/SH3 domain structure and binding of the regulatory carboxyl-terminal peptide containing tyrosine 505 to the contact area of the two Lck molecules(29) . At the cell membrane, such a closed conformation could block the catalytic domains of Lck and inactivate the enzyme. The authors suggest that ligand binding to the SH2 or SH3 domain of Lck might induce the open, enzymatically active state(29) . We have shown that Tip is phosphorylated on tyrosine residues in transfected T cells, and that it can be phosphorylated by Lck on tyrosine residues in cell-free systems(8) . But phosphotyrosine does not interfere with the association of Tip with Lck making involvement of the SH2 domain of Lck unlikely. However, interaction of the class II SH3 binding motif with the SH3 domain of Lck could induce the active open conformation of the enzyme.

Polyoma virus middle T antigen is another viral protein which binds and activates Src family kinases, namely p60, p62, and p59(30) . Besides Src family kinases, middle T binds a multitude of cellular proteins involved in signal transduction, the function of which seems to converge in the activation of the mitogen-activated protein kinase pathway(30) . Whether there are other signaling molecules associated with Tip remains to be discovered. It could be that the oncoprotein StpC complements Tip so that the two factors together achieve a function comparable to that of polyoma middle T antigen.

The relevance of Tip-induced activation of p56 for the transformation of human T cells by H. saimiri remains to be finally proven. Our data support the notion that in the presence of the oncoprotein StpC the action of Tip on the T cell kinase p56 could be a decisive factor. This would explain the T cell selectivity of the transformation by H. saimiri.


FOOTNOTES

*
This work was financed in part by the Bundesministerium für Forschung und Technologie (Projekt Autoimmunität). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank(TM)/EMBL Data Bank with accession number(s) M55264[GenBank].

§
To whom correspondence should be addressed. Tel.: 49-40-311-82-240; Fax: 49-40-311-82-386.

(^1)
The abbreviations used are: IL-2, interleukin-2; GST, glutathione S-transferase; HTLV-I, human T lymphotropic virus type I; PAGE, polyacrylamide gel electrophoresis; Stp, saimiri transformation-associated protein; Tip, tyrosine kinase interacting protein; MOPS, 4-morpholinepropanesulfonic acid.

(^2)
J. B. Bolen, unpublished observations.

(^3)
N. Wiese and B. M. Bröker, unpublished observations.

(^4)
B. M. Bröker, unpublished observation.


ACKNOWLEDGEMENTS

We thank B. Biesinger for cell line CB15, beta-Gal-Tip, and the p488PX plasmid, H. Fickenscher for cell line C3 and for the anti-Tip serum, H. Karasuyama for the BCMGSNeo vector, and B. Malissen for the BW cell line. C. Steeg and A. Hutloff are gratefully acknowledged for performing the transfection experiments, and K. Breuer for generation of the monoclonal antibody BNI2.


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