©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
Prosomatostatin Processing in Permeabilized Cells
CALCIUM IS REQUIRED FOR PROHORMONE CLEAVAGE BUT NOT FORMATION OF NASCENT SECRETORY VESICLES (*)

(Received for publication, July 12, 1995; and in revised form, October 26, 1995)

Cary D. Austin (1)(§) Dennis Shields (1) (2)(¶)

From the  (1)Departments of Developmental and Molecular Biology and (2)Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

Our laboratory has been using a permeabilized cell system derived from rat anterior pituitary GH(3) cells expressing prosomatostatin (pro-SRIF) to study prohormone processing and nascent secretory vesicle formation in vitro. Because calcium is necessary for prohormone processing enzyme activity, secretory granule fusion with the plasma membrane, and possibly sorting to the regulated pathway, we treated permeabilized cells with the calcium ionophore A23187 to determine the role of calcium in pro-SRIF cleavage and nascent vesicle formation from the trans-Golgi network (TGN). Here we demonstrate that pro-SRIF cleavage was markedly inhibited when lumenal free calcium was chelated with EGTA in the presence of A23187. Surprisingly, submillimolar free calcium (15 µM) was sufficient to maintain prohormone cleavage efficiency, a value far lower than that estimated for total calcium levels in the TGN and secretory granules. Experiments using both A23187 and the protonophore CCCP revealed that free calcium is absolutely required for efficient pro-SRIF cleavage, even at the optimal pH of 6.1. Secretory vesicle formation by contrast was not inhibited by calcium chelation but rather by millimolar extralumenal free calcium. Together, these observations demonstrate that pro-SRIF processing and budding of nascent secretory vesicles from the TGN can be uncoupled and therefore have distinct biochemical requirements. Interestingly, our data using intact GH(3) cells demonstrate that basal secretion of SRIF-related material is largely calcium-dependent and therefore cannot be equated with constitutive pathway secretion. These results underscore the importance of determining calcium requirements before assigning a secretion event to either the constitutive or regulated secretory pathway.


INTRODUCTION

The secretory pathway in eukaryotes consists of a series of topologically distinct intracellular membrane-enclosed compartments that are specialized in composition and function. Vesicular transport permits sorting without compromising compartmental identity. In polypeptide hormone-producing cells, the trans-Golgi network (TGN) (^1)has been implicated in specific protein trafficking and endoproteolytic maturation events in the distal secretory pathway (for review see Shields and Danoff (1993)). These cells can store and concentrate hormones in dense core secretory granules that deliver their contents to the cell surface by calcium-dependent fusion with the plasma membrane after an appropriate extracellular stimulus. In addition, neuroendocrine cells possess the ubiquitous constitutive pathway, whereby secretory proteins are continually delivered to the cell surface in a calcium-independent manner (Turner et al., 1992; Miller and Moore, 1991). Morphological evidence indicates that sorting of nascent secretory proteins into the regulated versus constitutive pathway occurs in the TGN (Sossin et al., 1990; Orci et al., 1987), although recent studies suggest that in pancreatic cells sorting may also occur after TGN exit in immature secretory granules through a pathway called ``constitutive-like secretion'' (Kuliawat and Arvan, 1994; Arvan and Castle, 1992). Biochemical evidence supports a model whereby the low pH and high calcium in the TGN lumen triggers selective aggregation and hence sorting of proteins destined for secretory granules (Song and Fricker, 1995; Colomer et al., 1994; Chanat and Huttner, 1991), whereas constitutively secreted proteins are excluded from such aggregates.

Lumenal ionic conditions also appear to play an important role in hormone activation. Many peptide hormones are initially synthesized as larger inactive polyprotein precursors or prohormones that undergo endoproteolytic maturation during intracellular transport. Prohormone cleavage occurs within the TGN and secretory granules at a defined set of basic residues by the recently discovered prohormone convertase enzymes (PCs) (for reviews see Seidah et al.(1993) and Steiner et al.(1992)). PC1/3 and PC2, which are the predominant PCs expressed in neuroendocrine tissue, are calcium-dependent endoproteases that require an acidic pH for maximal activity (Shennan et al., 1995; Rhodes et al., 1993; Davidson et al., 1988).

To investigate prohormone sorting, endoproteolytic maturation, and secretory vesicle formation in vitro, we have developed a permeabilized cell system (Xu and Shields, 1994, 1993) derived from growth hormone and prolactin secreting rat anterior pituitary GH(3) cells stably expressing human or anglerfish prosomatostatin (pro-SRIF) (Elgort and Shields, 1994; Stoller and Shields, 1988). These cells possess significant levels of PC2 and cleave pro-SRIF with 70% efficiency to generate the amino-terminal 75-80 amino acid propeptide and the carboxyl-terminal 14-amino acid mature SRIF (Elgort and Shields, 1994; Stoller and Shields, 1988). Pro-SRIF cleavage in permeabilized GH(3) cells requires ATP to generate an acidic TGN lumenal pH (Xu and Shields, 1994). For concomitant formation of nascent secretory vesicles, both ATP and GTP are required (Xu and Shields, 1993).

We have employed the permeabilized cell system in conjunction with the calcium ionophore A23187 to examine the role of free calcium in prohormone processing and vesicle formation from the TGN. Here, we demonstrate that lumenal free calcium is absolutely required for efficient pro-SRIF cleavage but not for secretory vesicle formation. We also show that lumenal ionic conditions permissive for pro-SRIF cleavage in the TGN are nonpermissive extralumenally for vesicle formation.


EXPERIMENTAL PROCEDURES

Materials

[S]Methionine was purchased from Amersham Corp. EGTA was purchased from J. T. Baker, Inc. A23187, CCCP, and BAPTA were purchased from Calbiochem. Rabbit anti-growth hormone serum (Xu and Shields, 1993), anti-SRIF-propeptide serum, which recognizes prepro-SRIF, pro-SRIF, and the free propeptide (Elgort and Shields, 1994), and anti-SRIF serum (RSS1), which recognizes prepro-SRIF, pro-SRIF, and the carboxyl-terminal mature SRIF-14 (Warren and Shields, 1984), were described previously. Rabbit anti-prolactin serum was raised against a peptide corresponding to residues 200-213 of rat prolactin (CLRRDSHKVDNYLK), which was cross-linked to maleimide-activated hemocyanin (Pierce). Rat anterior pituitary GH(3) cells stably expressing human prepro-SRIF (GH(3).Hu.S) were described previously (Elgort and Shields, 1994).

Cell Culture and Pulse Labeling

GH(3).Hu.S cells were grown and pulsed with 500 µCi/ml [S]methoinine, which labels the amino-terminal propeptide of pro-SRIF as described previously (Stoller and Shields, 1988), and chased in the presence of complete growth medium for 2 h at 19 °C. These conditions result in retention of 95% of the radiolabeled hormones in the TGN (Xu and Shields, 1993). Cells were then placed on ice prior to subsequent treatment.

Permeabilized Cell Preparation and in Vitro Incubations

Preparation of mechanically permeabilized cells (>95% breakage as assessed by trypan blue staining) was described previously (Xu and Shields, 1993). The standard incubation condition for these experiments contained 5 times 10^5 permeabilized cells, 25 mM Hepes-KOH, pH 7.3, 125 mM KCl, 2.5 mM MgCl(2), 1 mM ATP, 200 µM GTP, 10 mM creatinine phosphate, 160 µg/ml creatinine phosphate kinase (ATP regenerating system), 0.5 mM phenylmethylsulfonyl fluoride, and 5 µg/ml trasylol in 300 µl. Background free calcium was determined to be 10-20 µM by measurement with a calcium-selective electrode (see below). Incubation for 2 h at 37 °C under these conditions is sufficient for both pro-SRIF maturation and nascent secretory vesicle formation (Xu and Shields, 1993). Because our previous studies demonstrated that pro-SRIF processing can be effectively monitored by measuring the consumption of pro-SRIF concomitantly with the accumulation of either the free propeptide by SDS-polyacrylamide gel electrophoresis or mature SRIF by HPLC (Elgort and Shields, 1994), we used the former protocol for these experiments. For incubations in the presence of A23187 (dissolved in dimethyl sulfoxide) or CCCP (dissolved in ethanol), final concentrations of solvent in the reactions did not exceed 0.5%. For incubations at pH 6.1, Hepes in the assay buffer was replaced by 25 mM MES, pH 6.1.

Intact Cell Incubations

After the 19 °C chase, cells were incubated for 10 min at 4 °C followed by 2 h at 37 °C in buffer containing 25 mM Hepes, pH 7.3, 130 mM NaCl, 3.2 mM KCl, 0.6 mM MgSO(4), 1.3 mM KH(2)PO(4), 6 mM glucose, 0.1% bovine serum albumin, and either 10 mM EGTA, 10 mM CaCl(2), or background levels of free calcium determined to be 10-50 µM by measurement with a calcium-selective electrode (see below) in the presence or the absence of 5 µM A23187.

Free Calcium Measurements

Background free calcium concentrations were determined with a calcium-selective electrode (Philips IS 561) equipped with a fresh poly(vinyl chloride) membrane. The potential difference between the calcium electrode and a reference electrode (double-junction Orion) was monitored with an Orion Expandable ionAnalyzer EA 920 meter. The calibration curve of this electrode was linear between 10 and 10M CaCl(2).

Immunoprecipitation and Densitometry

Sedimented intact or permeabilized cell pellets were lysed in 100 µl of phosphate-buffered saline containing 0.5% Nonidet P-40, 0.5% sodium deoxycholate, and a mixture of protease inhibitors as described previously (Stoller and Shields, 1988). Lysates and supernatants containing secreted or budded material were treated sequentially with each antisera in a buffer containing 2.5% Triton X-100 and precipitated with protein A-Sepharose as described previously (Xu and Shields, 1993). Immunoreactive material was resolved by SDS-polyacrylamide gel electrophoresis and detected by fluorography. Band intensities were quantitated using a Molecular Dynamics Model 300A computing densitometer and Image Quant 3.3 software (Molecular Dynamics, Sunnyvale, CA).


RESULTS

Lumenal Free Calcium Is Required for Endoproteolytic Maturation of pro-SRIF

To determine the free calcium requirement for pro-SRIF processing in permeabilized cells, the calcium concentration of the assay buffer was manipulated in the presence or the absence of the calcium ionophore A23187. As shown in Fig. 1, pro-SRIF cleavage efficiency was not significantly affected by perturbations of extralumenal calcium in the absence of A23187. Our standard in vitro incubation, which permits 35-50% pro-SRIF cleavage (Fig. 1B; Xu and Shields (1994)), includes only trace levels (15 µM) of free calcium. Chelation of this extralumenal free calcium with EGTA did not significantly alter cleavage efficiency (Fig. 1, A and B, hatched bars). Similarly, addition of up to 10 mM free calcium, a concentration similar to estimates for total (free plus bound) calcium in the TGN lumen (Chanat and Huttner, 1991), had little effect on pro-SRIF processing. However, chelation of free calcium with EGTA markedly inhibited pro-SRIF cleavage when A23187 was included to access the lumenal free calcium pools (Fig. 1, A and B, black bars). In contrast, cleavage efficiency was maintained in the presence of A23187 when free calcium was included in the incubations. Surprisingly, the trace free calcium of our standard incubation maintained efficient pro-SRIF cleavage in the presence of A23187. To eliminate the unlikely possibility that EGTA was inhibiting pro-SRIF cleavage by a mechanism other than chelation of free calcium, both EGTA and calcium were included simultaneously in the presence of A23187 (data not shown). Pro-SRIF cleavage was not inhibited by EGTA in these experiments, provided that the amount of added calcium was equal to or greater than that of EGTA (i.e. 50 µM free calcium or greater, as determined with a calcium-selective electrode). These results demonstrate that lumenal free calcium in the low micromolar range was sufficient for efficient endoproteolytic maturation of pro-SRIF in permeabilized cells. We cannot exclude the possibility, however, that a lower free calcium concentration would also support processing.


Figure 1: Calcium requirement for pro-SRIF cleavage in vitro. A, cells were pulse-labeled with [S]methionine for 12 min at 37 °C, chased for 2 h at 19 °C, and permeabilized. Permeabilized cells were then preincubated with or without 1 µM calcium ionophore A23187 in the presence of 2 mM EGTA or 0.015 (standard condition), 0.5, 2.0, 5.0, or 10.0 mM additional calcium chloride for 10 min at 4 °C, followed by a further incubation with ATP and GTP for 2 h at 37 °C. Samples were then separated into pellet (P) and supernatant (S) fractions to monitor vesicle formation (see Fig. 4) by centrifugation at 15,000 times g for 10 s and immunoprecipitated with rabbit anti-SRIF-propeptide or anti-SRIF (RSS1) serum (see ``Experimental Procedures''). Immunoreactive peptides were resolved by SDS-polyacrylamide gel electrophoresis (20% acrylamide) and detected by fluorography. B, cleavage of pro-SRIF in permeabilized cells requires submillimolar lumenal free calcium. Quantitation by densitometry of fluorograms was as in A. Pro-SRIF cleavage efficiency = ([sum of the propeptide band intensities from the pellet and supernatant] [sum of the pro-SRIF and propeptide band intensities from the pellet and supernatant]) times 100. The values represent means ± S.E. from at least three experiments. Incubations with up to 10 mM EGTA resulted in similar cleavage efficiencies to those with 2 mM EGTA (not shown).




Figure 4: Nascent secretory vesicle formation does not require free calcium and is inhibited by millimolar extralumenal free calcium. The pellet and supernatant (nascent vesicle) fractions from Fig. 1were treated sequentially with anti-SRIF-propeptide, anti-prolactin, and growth hormone antisera and the immunoprecipitated material quantitated by densitometry of fluorograms. Budding efficiency = ([sum of the hormone band intensities from the supernatant] [sum of the hormone band intensities from the pellet and supernatant]) times 100. The values represent the means ± S.E. from at least three experiments. Budding efficiency in the absence of A23187 is shown and was not significantly affected by inclusion of 1 µM A23187 (Fig. 1A and data not shown).



To confirm the lumenal calcium requirement for pro-SRIF cleavage in vivo, intact cells were incubated at 37 °C with or without free calcium in the presence of A23187 following a 2 h chase at 19 °C. Consistent with the in vitro results, chelation of cellular free calcium with EGTA in the presence of A23187 inhibited pro-SRIF cleavage (Fig. 2). Pro-SRIF cleavage efficiency in the presence of A23187 was significantly higher in samples containing trace (10-50 µM) or 10 mM added calcium, although for unknown reasons, processing was not completely restored to control levels. Thus lumenal free calcium in the low micromolar range was also sufficient for endoproteolytic maturation of pro-SRIF in intact cells.


Figure 2: Cleavage of pro-SRIF in intact cells requires submillimolar calcium. Cells were pulse-labeled with [S]methionine for 12 min at 37 °C, chased for 2 h at 19 °C, and then preincubated with or without 5 µM A23187 in the presence of 10 mM EGTA, 0.03 mM (con), or 10.0 mM calcium chloride for 10 min at 4 °C, followed by a further incubation for 2 h at 37 °C. Pro-SRIF cleavage efficiency was determined by immunoprecipitation from cell lysates and media followed by densitometric quantitation of the fluorograms. The values represent the means ± S.E. from at least three experiments.



Our earlier studies demonstrated that a pH of 6.0-6.2 is necessary for pro-SRIF cleavage in the TGN (Xu and Shields, 1994), a value that corresponds to estimates of the endogenous lumenal pH in the TGN (Seksek et al., 1995; Yilla et al., 1993; Anderson and Orci, 1988). To determine if the lumenal free calcium requirement for pro-SRIF cleavage is independent of the pH requirement, permeabilized cells were incubated under varied calcium and pH conditions in the presence of both A23187 and the protonophore CCCP simultaneously (Fig. 3, black bars). When lumenal calcium was chelated with EGTA at the suboptimal pH of 7.3 (double nonpermissive condition), cleavage was inhibited. In contrast, when millimolar free calcium was present at the optimal pH of 6.1 (double permissive condition), pro-SRIF cleavage was 45-50% efficient. Chelation of lumenal free calcium with EGTA at the optimal pH of 6.1 (single nonpermissive condition) only partially inhibited pro-SRIF cleavage because the affinity of EGTA for calcium is strongly pH-dependent and greatly diminished at low pH (Bers et al., 1994). The calcium chelator BAPTA exhibits a much lower pH sensitivity and consequently is more effective at pH 6.1 (Bers et al., 1994). When permeabilized cells were treated with BAPTA at the optimal pH of 6.1, pro-SRIF maturation was potently inhibited (<10% cleavage). As observed for the single ionophore experiments, trace micromolar free calcium maintained efficient pro-SRIF cleavage at pH 6.1 in the presence of A23187 and CCCP (data not shown). Thus even at the optimal pH of 6.1 lumenal free calcium (no more than 15 µM) is absolutely required for efficient endoproteolytic maturation of pro-SRIF. In contrast to our previous single ionophore experiments with CCCP at pH 7.3 (Xu and Shields, 1994), inhibition of cleavage at this pH was not complete in the presence of both ionophores and 10 mM calcium (data not shown) for reasons that are unclear at present. Nevertheless, our data here demonstrate that although acidic lumenal pH is important, lumenal free calcium is absolutely required for efficient pro-SRIF cleavage. Cleavage efficiency was much less sensitive to calcium and pH manipulations when ionophores were omitted from the incubations (Fig. 3, hatched bars), consistent with the ability of the TGN and nascent secretory vesicles of the permeabilized cells to maintain a relatively stable lumenal environment in the presence of variations in the extralumenal environment.


Figure 3: Lumenal free calcium and pH requirements for pro-SRIF cleavage in vitro. Cleavage of pro-SRIF in permeabilized cells at optimal pH requires calcium. Permeabilized cells were preincubated with or without 1 µM A23187 and 30 µM CCCP at pH 7.35 or 6.1 in the presence of either 2 mM EGTA, 2 mM BAPTA, or 10.0 mM calcium chloride for 10 min at 4 °C, followed by a further incubation with ATP and GTP for 2 h at 37 °C. Pro-SRIF cleavage efficiency was determined by immunoprecipitation followed by SDS-polyacrylamide gel electrophoresis and densitometry. The values represent the means ± S.E. from at least three experiments.



Free Calcium Is Not Required for Nascent Secretory Vesicle Formation

To determine the free calcium requirement for nascent vesicle formation, we monitored budding efficiency from the TGN in vitro (Fig. 4). Vesicle budding from the TGN was very efficient (35-50% for growth hormone, prolactin, and pro-SRIF) when extralumenal calcium was chelated with EGTA in the absence of A23187 (Fig. 1A and Fig. 4). Identical results were obtained when incubations were performed in the presence of A21387 (Fig. 1A; data not shown). Our assay for vesicle budding is based on quantitating the release of radiolabeled hormones into a 15,000 times g supernatant following an in vitro incubation. To confirm that the appearance of radiolabeled hormones in the supernatant after incubation with A23187 and EGTA resulted from intact vesicle release rather than TGN lysis, sedimentation and protease protection assays were performed (Fig. 5). Most (66%) of the radiolabeled hormones appearing in the 15,000 times g supernatant after a standard incubation with EGTA (lane 2) could be sedimented at 200,000 times g (lane 4) with less (34%) remaining in the supernatant (lane 5). Furthermore, the radiolabeled hormones appearing in the 15,000 times g supernatant were protease-resistant (lane 7), whereas addition of detergent rendered them protease-sensitive (lanes 8 and 9). Thus the released radiolabeled hormones were membrane-enclosed and did not result from membrane lysis. We conclude that formation of nascent vesicles from the TGN of permeabilized cells does not require extralumenal nor lumenal free calcium. (^2)


Figure 5: Prolactin is packaged into sedimentable and membrane-enclosed secretory vesicles. Permeabilized cells were incubated as in Fig. 1with 1 µM A23187 in the presence of 2 mM EGTA. One sample (CON) was separated into 15,000 times g pellet (lane 1) and supernatant (lane 2) fractions; a second sample (SED) separated into 15,000 times g pellet (lane 3) and supernatant fractions. This supernatant was then centrifuged at 200,000 times g for 20 min to generate a second pellet (lane 4) (nascent vesicle) and supernatant (lane 5) fractions. Samples PtK and PtK/Det were incubated with 25 µg/ml proteinase K in the absence or the presence of 1% Triton X-100, respectively, for 15 min at 4 °C, quenched with 1 mM phenylmethylsulfonyl fluoride, and separated into 15,000 times g pellet (lanes 6 and 8) and supernatant (lanes 7 and 9) fractions. Budding efficiency was determined by immunoprecipitation using rabbit anti-prolactin serum. For the SED sample, budding efficiency is defined as ([prolactin band intensity from the SP fraction] [sum of the prolactin band intensities from the P, SP, and SS fractions]). Similar results were obtained for budding of growth hormone and SRIF propeptide + pro-SRIF (not shown).



Free Calcium Inhibits Nascent Secretory Vesicle Formation in Vitro but Is Required for Efficient Basal Secretion of pro-SRIF in Vivo

Formation of nascent secretory vesicles from the TGN of permeabilized cells was strongly inhibited by millimolar concentrations of extralumenal free calcium ( Fig. 1and Fig. 4). This inhibitory effect of millimolar calcium was not due to nonspecific vesicle aggregation or adherence to the permeabilized cells. (^3)Inhibition was half-maximal at 2-5 mM free calcium independent of the presence of A23187 (Fig. 1A and Fig. 4, and data not shown). To determine if calcium was also inhibitory in vivo, we monitored basal secretion from intact cells (Fig. 6). In contrast to the observations in vitro, basal hormone secretion from intact cells in the presence of A23187 was not inhibited by millimolar free calcium (Fig. 6). Thus the calcium-sensitivity of TGN exit in vitro could not be recapitulated in vivo. Interestingly, basal secretion of pro-SRIF and the pro-SRIF propeptide was dramatically inhibited in the presence of A23187 when free calcium was chelated with EGTA (68 ± 7% inhibition, Fig. 6, hatched bars). Inhibition was less pronounced for prolactin (40 ± 13% inhibition, Fig. 6, shaded bars) and growth hormone (50% inhibition, data not shown). Trace free calcium (10-50 µM) was sufficient to maintain efficient secretion in the presence of A23187. Thus free calcium is required for efficient basal secretion, particularly for pro-SRIF and the SRIF propeptide.


Figure 6: Basal secretion of pro-SRIF and prolactin from intact cells requires calcium. Cells were pulse-labeled with [S]methionine for 12 min at 37 °C, chased for 2 h at 19 °C, and then preincubated with or without 5 µM A23187 in the presence of 10 mM EGTA, 0.03 mM (con) or 10.0 mM calcium chloride for 10 min at 4 °C, followed by a further incubation for 2 h at 37 °C. Secretion efficiency was determined by immunoprecipitation from cell lysates and media followed by densitometric quantitation of the fluorograms and is defined as ([hormone band intensity from the medium fraction] [sum of the hormone band intensities from the medium and cell lysate fractions]). The values represent the means ± S.E. from at least three experiments.




DISCUSSION

An advantage of using a permeabilized cell system to study organelle function is that cytosolic conditions can be directly manipulated without necessarily changing conditions within the lumen of the organelle. Our permeabilized cell system allows us to examine an important event in the lumen of the TGN and immature secretory granules: the endoproteolytic maturation of a prohormone. In addition, this system permits examination of an isolated step in the transport of hormones from the TGN to the cell surface: the formation of nascent secretory vesicles. In this study, we exploited the permeabilized cell system to (a) determine the lumenal free calcium requirement for endoproteolytic maturation of pro-SRIF, (b) compare the requirements for low pH versus calcium for pro-SRIF processing, and (c) determine the effect of perturbed calcium concentration and pH on the formation of vesicles from the TGN. Our data demonstrate that low levels (15 µM) of lumenal free calcium are sufficient for efficient endoproteolytic maturation of pro-SRIF in permeabilized GH(3) cells (Fig. 1, A and B). This is independent of the requirement for low pH, because inefficient cleavage occurred at the optimal pH of 6.1 when lumenal free calcium was chelated with BAPTA (Fig. 3). The requirement for submillimolar free calcium could also be demonstrated in intact cells (Fig. 2).

In light of the high levels of intralumenal calcium (estimated to be 10 mM or higher) in the TGN and secretory granules (Chanat and Huttner, 1991), it was surprising that such low concentrations of free calcium were sufficient to support pro-SRIF cleavage. However, much of this calcium may in fact be bound to high capacity, low affinity calcium-binding proteins such as the chromogranins (Rosa et al., 1992; Huttner et al., 1991; Reiffen and Gratzl, 1986). Indeed, studies using a calcium-selective electrode suggest that in chromaffin granules, only 24 µM calcium exists in the free unbound state (Bulenda and Gratzl, 1985). Thus our present observations showing a low micromolar free calcium requirement for pro-SRIF cleavage is consistent with earlier measurements of endogenously available lumenal free calcium in regulated secretory granules. We do not know if the exchange between free and protein-bound pools of calcium is sufficiently rapid to permit depletion of the protein-bound pool when free calcium is chelated during our incubations with A23187, although laser scanning confocal and ion microscopy studies revealed significant (70-90%) depletion of total lumenal calcium in the Golgi of porcine kidney LLC-PK(1), mouse fibroblast 3T3, and rat myoblast L5 cells by 5 min using EGTA in the presence of A23187 (Chandra et al., 1991). Thus the free calcium requirement for pro-SRIF cleavage in our system could be indirect through maintenance of the protein-bound lumenal pool.

A direct requirement for submillimolar free calcium is consistent with the observed in vitro properties of PC2, the enzyme likely responsible for pro-SRIF cleavage in our system (Brakch et al., 1995; Seidah et al., 1994; Xu and Shields, 1994; Mackin et al., 1991). Using PC2 purified from rat insulinoma granules, Hutton and colleagues observed low micromolar calcium optima for cleavage of purified chromogranin A (K 11 µM) (Arden et al., 1994), proopiomelanocortin (K = 5-80 µM) (Rhodes et al., 1993), and proinsulin (K = 100 µM) (Bennett et al., 1992). The calcium optimum for cleavage by PC2 synthesized in vitro using Xenopus egg extracts, however, was higher (K = 1-4 mM) (Shennan et al., 1995). In contrast to PC2 purified from granules, PC1 displayed a much higher calcium optimum in vitro (K = 2.5 mM for proinsulin cleavage; Davidson et al.(1988) and Zhou and Lindberg(1993)).

Our data also demonstrate that free calcium is not required for vesicle formation from the TGN of permeabilized cells (Fig. 1A and 4). A similar observation was made previously using permeabilized MDCK cells (Bennett et al., 1988). Interestingly, a recent study investigating the sorting of secretogranin II from the TGN of permeabilized PC12 cells (Carnell and Moore, 1994) suggests that lumenal free calcium may not be required for sorting. In our system, formation of vesicles from the TGN was strongly inhibited by extralumenal free calcium roughly 5 orders of magnitude greater than physiological cytoplasmic conditions (Fig. 1A and Fig. 4). We were unable to demonstrate a calcium block on TGN exit in intact cells (Fig. 6) and presume that in permeabilized cells, millimolar calcium nonspecifically disrupts the TGN budding machinery. Interestingly, elevated cytosolic calcium levels (100 µM) in permeabilized cells can cause mistargeting of plasma membrane clathrin-coated vesicle adaptor complexes (Seaman et al., 1993). We also found that an acidic extralumenal pH of 6.1 strongly inhibits both budding in permeabilized cells and secretion in intact cells (not shown). Inhibition of clathrin-mediated transport events from the TGN and plasma membrane by cytosol acidification is well documented (Hansen et al., 1993; Cosson et al., 1989; Heuser and Anderson, 1989; Heuser, 1989; Sandvig et al., 1987) and appears to involve reversible structural alterations in the clathrin cages.

Significantly, our results reveal that pro-SRIF endoproteolytic maturation and entry into nascent secretory vesicles are not obligatorily coupled events (compare Fig. 1B and Fig. 4). Pro-SRIF cleavage was inhibited by chelation of lumenal free calcium, whereas formation of nascent vesicles was unimpaired. Conversely, vesicle formation but not pro-SRIF cleavage was inhibited by raising extralumenal free calcium to millimolar levels or by lowering the pH, which reinforces our previous finding that pro-SRIF endoproteolytic maturation can proceed in the TGN if exit is prevented (Xu and Shields, 1993). These observations contrast with recent studies using pancreatic islets (Huang and Arvan, 1994), which suggested that proinsulin endoproteolytic maturation cannot proceed if TGN exit is blocked. It is not clear if this discrepancy is due to tissue- or substrate-specific differences or methodological differences. It is noteworthy that proinsulin processing requires both the activities of PC1 and PC2, the former having significantly higher calcium and lower pH requirements than the latter (Zhou and Lindberg, 1993; Rhodes et al., 1993; Davidson et al., 1988). Overall, our data demonstrate that by using the permeabilized cell system, prohormone endoproteolytic maturation can be dissociated from entry into secretory vesicles.

Inhibition of pro-SRIF basal secretion from intact cells by chelation of free calcium, and to a lesser extent that of prolactin and growth hormone, reveals a calcium-dependent transport step distal to TGN exit (Fig. 6). An important distinction must be made between basal secretion and constitutive pathway secretion. Distal steps in constitutive secretion do not require calcium (Turner et al., 1992; Miller and Moore, 1991). In the regulated pathway by contrast, calcium-dependent fusion of secretory vesicles with the plasma membrane has been well documented (Turner et al.(1992) and Martin and Walent(1989); for review see Kelly (1993)). We propose that calcium-dependent basal secretion is in fact secretion by the regulated pathway rather than the constitutive pathway. It is conceivable that the threshold for activation of the regulated pathway has been diminished in immortalized cell lines such as GH(3), permitting some secretion by this pathway even in the absence of secretagogues. Consistent with this hypothesis, two-thirds of basally secreted SRIF-related material was released in a calcium-dependent manner (Fig. 6). If this material were largely derived from the regulated pathway, it should have undergone significant processing. Indeed, we found that two-thirds of basally released SRIF-related material appeared as free propeptide (67 ± 9%, data not shown) and mature SRIF (60%, Stoller and Shields, 1988). Accordingly, we feel that calcium dependence more precisely defines regulated secretion than secretagogue stimulation above basal levels.


FOOTNOTES

*
This work was supported by National Institutes of Health Grant DK21860 and in part by a grant from the Juvenile Diabetes Foundation (to D. S.). Core support was provided by National Institutes of Health Cancer Center Grant P30CA13330. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
Supported by a Life and Health Insurance Medical Research Fund M.D.-Ph.D. Scholarship.

To whom correspondence should be addressed: Dept. of Developmental and Molecular Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461. Tel.: 718-430-3306; Fax: 718-430-8567; shields@aecom.yu.edu.

(^1)
The abbreviations used are: TGN, trans-Golgi network; BAPTA, 1,2-bis(2-aminophenoxy)ethane-N,N,N`,N`-tetraacetic acid; CCCP, carbonyl cyanide m-chlorophenylhydrazone; SRIF, somatostatin; PC, prohormone convertase enzyme; MES, 4-morpholineethanesulfonic acid.

(^2)
A requirement for low nanomolar free calcium (Ronning and Martin, 1986), however, cannot be excluded.

(^3)
C. D. Austin and D. Shields, unpublished data.


ACKNOWLEDGEMENTS

We thank Dr. Wai Lam Wong and Yeguang Chen for helpful suggestions and discussions.


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