©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
The C-terminal Extension of Yeast Seryl-tRNA Synthetase Affects Stability of the Enzyme and Its Substrate Affinity (*)

(Received for publication, July 5, 1995; and in revised form, November 10, 1995)

Ivana Weygand-Durasevic (3) (2) Boris Lenhard (3) (2) Sanda Filipic (3) (2) Dieter Söll (1)(§)

From the  (1)From theDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520-8114, the (2)Department of Biochemistry, Faculty of Science, University of Zagreb, 10000 Zagreb, Croatia, and the (3)Department of Molecular Genetics, Rudjer Boskovic Institute, 10000 Zagreb, Croatia

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

Saccharomyces cerevisiae seryl-tRNA synthetase (SerRS) contains a 20-amino acid C-terminal extension, which is not found in prokaryotic SerRS enzymes. A truncated yeast SES1 gene, lacking the 60 base pairs that encode this C-terminal domain, is able to complement a yeast SES1 null allele strain; thus, the C-terminal extension in SerRS is dispensable for the viability of the cell. However, the removal of the C-terminal peptide affects both stability of the enzyme and its affinity for the substrates. The truncation mutant binds tRNA with 3.6-fold higher affinity, while the K for serine is 4-fold increased relative to the wild-type SerRS. This indicates the importance of the C-terminal extension in maintaining the overall structure of SerRS.


INTRODUCTION

Aminoacyl-tRNA synthetases are essential enzymes that catalyze the esterification of an amino acid to its cognate tRNA with exquisite specificity. The combination of biophysical, biochemical and genetic techniques have significantly deepened our understanding of the structure and function of these enzymes(1, 2, 3) . The amino acid sequences of seryl-tRNA synthetases from Escherichia coli(4) , Thermus thermophilus(5) , Bacillus subtilis(6) , Coxiella burnetii(7) , Saccharomyces cerevisiae(8) , Chinese hamster (partial)(9) , and human (^1)are known. According to common structural motifs they are typical representatives of class II synthetases(10) . The crystal structures of two prokaryotic enzymes isolated from E. coli(11) and T. thermophilus(12) are quite similar, as is their mode of interaction with tRNA. We have been working with yeast SerRS, (^2)which shows only moderate similarity (about 30%) with prokaryotic seryl-tRNA synthetases on the level of the primary structure(5) , but still recognizes bacterial tRNA and can functionally substitute for the bacterial enzyme in vivo(13) . The overexpression of the yeast SES1 gene in E. coli generated high amounts of functional enzyme(13) , although with somewhat lower specific activity and slightly different electrophoretic mobility (^3)compared to the enzyme isolated from yeast. This raises the possibility that the yeast enzyme may not be modified or folded correctly in the bacterial host. In this paper we describe the purification of yeast SerRS from an overproducing strain of S. cerevisiae. The alignment of the primary sequences of all SerRS proteins reveals that the enzymes from yeast(8) , Chinese hamster(9) , and human^1 contain C-terminal extension between 20 and 48 amino acids long not found in prokaryotic synthetases. We speculated whether this peptide was important for maintaining the structure of the eukaryotic enzymes or if it had another function. Thus, we deleted the part of the S. cerevisiae SES1 gene encoding the short C-terminal domain and analyzed the expressed truncated protein.


MATERIALS AND METHODS

General Procedures

[^14C]Serine (180.5 Ci/mmol) and [^3H]ATP (40 Ci/mmol) were from Amersham Corp. YPD medium contained 1% yeast extract, 2% peptone, and 2% glucose(14) . Selection for yeast auxotrophic markers was done in a medium of 0.67% nitrogen base and 2% glucose lacking amino acids, supplemented as needed with adenine (20 µg/ml), uracil (20 µg/ml), and amino acids (20-30 µg/ml). Sporulation medium contained 1% potassium acetate and amino acids as needed for auxotrophic diploids. For induction of the GAL promoter, glucose was replaced by galactose (2%) in all liquid media and agar plates. Transformation of yeast was performed by the lithium acetate procedure or by electroporation(14) .

Strains and Plasmids

These are described in Table 1. SES1 was used for various purposes, and thus several different constructs were made. Plasmid pBRSES1(2) used for disrupting the SES1 gene (see scheme in Fig. 2) contained the genomic SES1 yeast DNA fragment inserted in the opposite orientation compared to pBRSES1(15) . For the construction of the centromeric plasmid pUN70SES1, the SalI/BamHI fragment of pBRSES1 was used. The truncation of the SES1 gene was carried out (see below) in pVTU-103SES1, where the BamHI cassette containing SES1(13) was inserted behind the ADH (alcohol dehydrogenase) promoter. The resulting gene, lacking the 60-bp encoding amino acids 443-462 of SerRS, was named SES1C20. For complementation of the null allele strain, a 2.1-kb SphI fragment containing SES1 or SES1C20 behind the ADH promoter was cloned into YEp351 (16) to generate YEp351SES1 and YEp351SES1C20, respectively. For overexpression, the BamHI cassettes with SES1 or SES1C20 were cloned behind the GAL promoter in pCJ11 (17) to generate the plasmids pCJ11SES1 and pCJ11SES1C20, respectively.




Figure 2: Disruption of the chromosomal SES1 gene. The plasmid pBRSES1(2) contains the coding region of the yeast SES1 gene (solid box) as well as the 5`- and 3`-noncoding regions (shaded box and arrow). In order to remove the HindIII site in the vector, plasmid was digested with SalI and religated. The internal 0.4-kb HindIII fragment of SES1 was replaced with the entire yeast LYS2 coding region (empty box), and the disrupted gene SES1::LYS2 was introduced into diploid yeast cells, as a BsmI/ClaI fragment, by electroporation. The rearrangement of the chromosomal region containing SES1 was confirmed by Southern blot, shown at the lower part of the figure. Genomic DNA was isolated from strain BR2727, before and after replacement of the chromosomal SES1 gene with the disrupted mutant allele (lanes 1 and 2, respectively). After digestion with BamHI, fragments were separated by gel electrophoresis, transferred to nitrocellulose, and probed with radioactively labeled entire SES1 gene. The position of the marker DNA fragments are shown on the left margin.



SES1 Gene Disruption

The experimental strategy (18) is presented in Fig. 2. In order to eliminate a HindIII site in the vector, pBRSES1(2) was digested with SalI and the larger fragment religated. The 0.4-kb HindIII fragment of the SES1 gene was excised and replaced with a LYS2 cartridge(19) . This is a 4.5-kb HindIII fragment with the LYS2 structural gene behind the CYC1 promoter. A 5-kb BsmI/ClaI fragment, in which the LYS2 gene was flanked by 137 and 379 bp of SES1 gene sequence, was then transformed by electroporation into S. cerevisiae diploid strain BR2727. Lys transformants were selected, sporulated, and tetrads dissected. Total DNA was also isolated from Lys transformants and used for Southern blot analysis.

In Vitro Mutagenesis

The SES1 gene was truncated by loop-out in vitro mutagenesis, with a 1:1 molar ratio of phosphorylated oligonucleotide to U-containing single-stranded pVTU-103SES1 DNA. The structure of the synthetic 37-mer was as follows: 5`-CATTCATGATATGCTATATTTATAAAAATTCTGGTTC-3`. After mutagenesis, DNA was transformed into E. coli strain DH5alpha and the plasmids with deleted SES1 were selected by colony hybridization using the mutagenic oligonucleotide as a probe.

Purification of Wild-type and Mutant SerRS from the Overproducing Strain

Yeast strains that overproduce full-length SerRS and its truncated mutant SerRSC20 were generated by transformation of S. cerevisiae S2088 by plasmids pCJ11SES1 and pCJ11SES1C20, respectively. The transformants were grown in glucose selective medium at 30 °C until saturation, and than diluted 25-fold with galactose-containing medium for promoter induction. The growth was continued until late exponential phase (A 2.5). In a typical experiment, the culture (1500 ml) was harvested by centrifugation (giving about 3 g of cells), washed with water, and resuspended in cold lysis buffer (100 mM Tris-HCl, pH 7.5, 5 mM DTT, 0.1 mM EDTA, and 0.2 mM PMSF) to a volume of 8 ml, followed by addition of an equal volume of glass beads (0.5 mm diameter). The disruption of cells was carried out in the bead beater, equipped with a cooling jacket, by four 15-s bursts, separated by 30 s of cooling. The cell breakage was over 90%. The beads were rinsed with the lysis buffer. The lysate was clarified by centrifugation at 100,000 times g for 90 min at 4 °C, giving about 10 ml extract with 10 mg of protein/ml. SerRS was purified by chromatography on DEAE-cellulose (DE52, Whatman) and phosphocellulose (P-11 Whatman) columns, as described previously(20, 21) . Protein extract was dialyzed against 20 mM potassium phosphate buffer, pH 7.2, containing 10% glycerol, 3 mM DTT, 0.1 mM EDTA, and 0.2 mM PMSF and applied onto a DEAE-cellulose column (1.5 times 14 cm) equilibrated in the same buffer. The column was developed with 60 ml of potassium phosphate gradient (20 mM, pH 7.2 to 250 mM, pH 6.8) in the presence of 10% glycerol, 3 mM DTT, 0.1 mM EDTA, and 0.2 mM PMSF. SerRS-containing fractions were detected by aminoacylation assay and SDS-PAGE, while in the case of SerRSC20 fractionation, the distribution of full-length and truncated SerRS in the fractions eluted from DEAE-cellulose column were detected by Western blots, using rabbit antiserum raised against wild-type SerRS (8) . The proteins in the pooled fractions were carefully desalted on a Sephadex G-25 column (PD-10, Pharmacia Biotech Inc.) and applied to a phosphocellulose column (1.6 times 5 cm) equilibrated in HEPES/KOH buffer, pH 7.0, containing 10 mM potassium chloride, 10% glycerol, 3 mM DTT, 0.1 mM EDTA, and 0.2 mM PMSF. The column was eluted with a 60-ml gradient of potassium phosphate (10-700 mM) in a buffer of the same composition as for the equilibration of the column.

Determination of Kinetic Parameters

The aminoacylation was done at 30 °C. Standard reaction mixtures contained 50 mM Tris-HCl, pH 7.5, 15 mM MgCl(2), 5 mM ATP, 4 mM DTT, 4 mg/ml unfractionated brewer's yeast tRNA (Boehringer Mannheim), and 25 µM [^14C]serine as described(13) , except that the serine concentration was increased up to 1 mM in selected experiments. Kinetic parameters (k and K(m)) of the aminoacylation reaction were determined with unfractionated yeast tRNA preparations containing 6% serine-accepting tRNAs(13) . The concentrations of serine-accepting tRNA ranged from 30 to 1000 nM in the reactions with wild-type SerRS and from 10 to 270 nM with SerRSC20. The K(m) values for ATP and serine were determined in the aminoacylation reaction by varying the concentration of studied substrate: ATP from 2 to 150 µM and [^14C]serine from 2 to 360 µM, respectively, while the other substrates were kept saturating. The concentrations of SerRS and SerRSC20 were 2-5 nM. The SerRS active site concentration was determined by the formation of the enzyme bound intermediate seryl-AMP according to Borel et al.(22) .

Southern and Northern Blot Hybridizations

Total yeast DNA was isolated from transformed and non-transformed yeast cells(14) . After digestion with BamHI, the fragments were separated on 0.7% agarose gel, transferred to nitrocellulose membrane and probed with 1.4-kb BamHI fragment (Table 1) containing the entire SES1 coding region. For Northern analysis, total yeast RNA was isolated by the guanidinium thiocyanate method(14) , separated on denaturing formaldehyde gel, transferred to Nytran membrane (Schleicher & Schuell), and hybridized to the entire SES1 gene, as described in the manufacturer's protocol.


RESULTS

Rationale

We wanted to test the functional and structural importance of the C-terminal extension of yeast SerRS, comprising 20 mostly basic and hydrophilic amino acids. Since this peptide is not a part of three highly conserved motifs characterizing class II synthetases(23) , and is not present in four prokaryotic SerRS enzymes (5, 7, 6, 24) (Fig. 1), a non-catalytic role was proposed. We therefore investigated charging by the truncated protein, both in vivo and in vitro, as well as its stability.


Figure 1: Schematic representation of the homology on the amino acid level between SerRS from E. coli, T. thermophilus, B. subtilis, C. burnetii, S. cerevisiae, Chinese hamster, and human. Numbers above the line indicate the position of the amino acids. The composition of the C-terminal extensions in the yeast enzyme (443-462) and the corresponding sequences of Chinese hamster and human SerRS (469-493) are shown by one-letter code. Only the sequence of 199 C-terminal amino acids of Chinese hamster SerRS is known. Horizontal bars indicate the approximate position of three motifs characteristic for class II synthetases. There is extensive homology between all SerRS proteins in motifs I, II, and III.



C-terminal Truncation of SerRS

A 60-bp fragment of the SES1 gene, coding for 20 C-terminal amino acids of SerRS, was deleted by in vitro loop-out mutagenesis using a discontinuously homologous oligonucleotide. The truncated protein SerRSC20 ends with Leu-442. It was expressed from a truncated SES1 gene, which has its natural TAA stop codon, followed by 19-bp of SES1 3`-flanking region up to the introduced BamHI site (see ``Materials and Methods''). The removal of the unusual, positively charged C-terminal peptide (amino acids 443-462; pI = 10.8), substantially changes the ionic properties of the whole enzyme: the calculated pI is 5.6 for the full-length protein and 5.3 for the truncated form.

Construction of S. cerevisiae SES1 Null Allele Strain

In order to test activity of the SES1 truncation mutant in vivo, we constructed the S. cerevisiae strain with inactivated SES1 allele, BR2727DeltaSES1 (Fig. 2). As previously shown by Southern blot analysis(8) , SES1 is a single-copy gene in the haploid genome, residing on a 15-kb BamHI fragment of chromosomal DNA. The SES1 gene contains an internal 0.4-kb HindIII fragment, which was replaced on the plasmid with a LYS2 cartridge, which also serves as a selectable marker. Lys transformants were selected, and the correct LYS2 integration at the SES1 locus was verified by Southern blot analysis (Fig. 2). Total DNA from BR2727DeltaSES1 and the parental strain BR2727 was digested with BamHI, separated by agarose gel electrophoresis, and transferred to nitrocellulose membrane. Hybridization with a radioactively labeled 1.4-kb BamHI fragment containing the entire SES1 structural gene, revealed the presence of one wild-type SES1 allele, giving a 15-kb BamHI fragment (Fig. 2, lane 1) of genomic DNA, while the other SES1 allele has been replaced in BR2727DeltaSES1 by the mutant allele SES1:LYS2 (Fig. 2, lane 2). Since the LYS2 gene carries an internal BamHI site, its integration into SES1 gives rise to two new BamHI fragments of 14 and 5 kb. Seven Lys isolates were sporulated and their tetrads dissected. All viable spores were lysine auxotrophs, and in no case did more than two of four separated spores form colonies. Inactivation of SES1 renders the cell inviable, indicating that the SES1 gene is essential and therefore indispensable for cell growth. The deletion could be rescued when SES1 was supplied on a centromeric or multicopy plasmid. Transformed diploids were sporulated and viable Lys haploids were isolated by the method of Rockmill et al.(25) . Selected haploids with a disrupted SES1 gene (Lys phenotype) where SerRS function was provided in trans (from a centromeric plasmid carrying wild-type SES1 gene), were used for complementation experiments via plasmid shuffling.

The C-terminal Extension Is Dispensable for Cell Viability

The recipient S. cerevisiae haploid BR2727DeltaSES1, where viability of the cell is ensured by a wild-type SES1 on the URA3-containing plasmid pUN70SES1, was cotransformed with YEp351SES1C20, which carries the LEU2-selectable marker and SES1C20 behind the ADH promoter. The function of the deleted gene was then assayed by complementation of SerRS function, by growing double transformants in the presence of uracil and replica plating the LysLeu colonies to 5-fluoroorotic acid containing medium(14) . This excludes the presence of the URA3-containing plasmid with the wild-type S. cerevisiae SES1 gene. The ability to select viable haploids carrying SES1C20, as the only source of seryl-tRNA synthetase activity, shows that 20 amino acids(443-462) at the C terminus of the enzyme are not required for the survival of the yeast cell, although the growth rate was significantly impaired (doubling time increased by 70%) when compared to BR2727DeltaSES1 complemented with the same plasmid carrying wild-type SES1.

Overexpression and Purification of Wild-type and Truncated Seryl-tRNA Synthetases from Yeast

In order to study the effect of removal of the C-terminal extension on the activity and the stability of the enzyme, full-length SerRS and mutant SerRSC20 were purified from yeast. To facilitate the purification of substantial amounts of proteins required for in vitro studies, we were interested in developing a high level overexpression system. Only 4-fold overproduction of SerRS was determined by Western blot analysis and aminoacylation with crude protein extract prepared from yeast cells transformed with pVTU103SES1 (Table 2). The expression of the truncated protein from pVTU103SES1C20 was negligible under the same experimental conditions. Induction of the GAL promoter on pCJ11SES1, transformed in strain S2088, resulted in 150-fold overproduction of full-length SerRS protein and a corresponding increase of the serylation activity measured in the crude protein extract (Fig. 3A, lanes a and b; Table 2). We found this system very suitable for the purification of the wild-type enzyme. However, in the protein extract made from induced yeast cells transformed with pCJ11SES1C20, the overproduction of truncated protein, as judged by scanning of the bands on the Western blot (not shown), was only 10 times above the control (extract made from non-induced cells) (Table 2) and thus undetectable on a Coomassie-stained gel (Fig. 3A, lanes c and d). In addition, the serylation activity of the extract, determined by standard aminoacylation assay (25 µM serine) was very low. It seems, therefore, that the C-terminal fragment of yeast SerRS influences the expression of the protein or its accumulation in the cell. This poor level of overexpression of the truncated protein, together with the necessity to separate the enzyme from endogenous wild-type SerRS present in the yeast strain S2088, made the purification procedure much more difficult and inefficient. pCJ11SES1C20 transformants of BR2727DeltaSES1 could not be used as a source of truncated protein, because the strain carries a mutation in the galactose permease gene and is not fully galactose inducible(26) .




Figure 3: The overexpression and purification of SerRS and SerRSC20 from yeast. A, an equal amount of protein extract (50 µg), prepared from cells transformed with the indicated plasmids under inducing and non-inducing conditions, was separated by SDS/8% PAGE and stained with Coomassie Brilliant Blue G250. Lane a, pCJ11SES1, non-induced; lane b, pCJ11SES1, induced; lane c, pCJ11SES1C20, non-induced; lane d, pCJ11SES1C20, induced. Lanes e and f contain full-length SerRS after purification on DEAE-cellulose and phosphocellulose columns (2 and 4 µg, respectively). B, chromosomally encoded SerRS and overproduced SerRSC20 were separated on a DE52 column. Aliquots (40 µl) from the eluted fractions were run on an SDS gel, and the proteins were electrophoretically transferred to nitrocellulose and probed with polyclonal antibodies against yeast SerRS(8) . The presence of the bound antibody was detected by anti-rabbit antibody conjugated with horseradish peroxidase. Lanes a and b, 100,000 times g supernatant from S2088/pCJ11SES1C20 (70 and 15 µg of protein, respectively); lanes c-m, DE52 fractions; lane n, pure SerRS, 0.2 µg. C, the separation of SerRSC20 on the P-11 column was followed by SDS-PAGE and silver staining of the gel. Lane a, DE52 fraction (same as k on panel B) loaded on the P-11 column; lanes b-d, three successive P-11 fractions with highest serylation activity. Black arrow indicates the position of full-length yeast SerRS; white arrow indicates the position of truncated protein. The position of the marker proteins are shown in the left margin (fructose-6-phosphate kinase, 84 kDa; pyruvate kinase, 58 kDa; fumarase, 48.5 kDa; lactate dehydrogenase, 36.5 kDa).



Full-length SerRS was purified to apparent homogeneity (Fig. 3A, lanes e and f) by a quick two-step chromatographic procedure that involves fractionation on DEAE-cellulose and phosphocellulose columns (20, 21) as the important steps in isolation of SerRS from non-overproducing yeast strains. The procedure allows the purification of 1 mg of protein from 1 g of yeast cells, with a specific activity of 115 nmol mg min, as determined in the standard aminoacylation assay with 25 µM serine. By increasing the concentration of serine to 1 mM (see below), a specific activity for the wild-type enzyme of 280 nmol mg min was obtained. The purified enzyme has two active sites for seryl-AMP per protein dimer (i.e. full site reactivity), as determined by the formation of the SerRS-bound intermediate seryl-AMP according to the method of Borel et al.(22) . The purification procedure for the truncated SerRS mutant was essentially the same as for SerRS, except the overall yield was 2 orders of magnitude lower (about 8 µg of protein/g of cells), both due to lower expression and to the narrow pools taken from both ion exchangers (see below). The separation of truncated enzyme from the wild-type SerRS by fractionation on DEAE-cellulose column was analyzed by the aminoacylation assay and by SDS-PAGE followed by Western blot analysis (Fig. 3B). The activity peak was found in fractions j and k. Since the best separation of full-length and truncated SerRS enzymes was found in fraction k, this material was applied on the phosphocellulose column after careful desalting. Truncated protein was eluted with 10 mM KCl, immediately following the majority of unbound proteins, indicating very weak interaction of SerRSC20 with the anionic resin. Fig. 3C shows the separation of the proteins from the P-11 eluted fractions on a silver-stained SDS gel. Apparently homogeneous truncated enzyme eluted in fraction d, was concentrated and used in additional experiments. When assayed in the presence of elevated serine concentration (1 mM), SerRSC20 shows specific activity similar to that of the full-length SerRS (257 nmol mg min).

Deletion of the C-terminal Peptide Affects the Kinetics of Serylation and Decreases Thermal Stability of the Protein

Kinetic parameters for wild-type SerRS and mutant SerRSC20 were determined in the aminoacylation reaction (Table 3). Due to the lack of pure yeast tRNA, the experiments were performed with bulk yeast tRNA, 6% of which was tRNA, thus only relative K(m) values for tRNA are meaningful. Mutant enzyme shows 3.6-fold higher affinity for tRNA at 30 °C. Interestingly, this increased affinity is not detectable at 37 °C, which is probably the result of a subtle, temperature-dependent, conformational change of the mutant enzyme (see below). Another difference in the kinetic parameters between SerRS and SerRSC20 is a 4-fold increased K(m) value for serine obtained for the mutant enzyme. There are no significant differences in k values for the substrates, with mutant and wild-type enzyme, when measured at high serine concentration (1 mM). It should be noted that our experiments revealed a higher K(m) value for serine for wild-type SerRS (Table 3) than reported earlier (10 µM)(27) , which was the reason for the increase in [^14C]serine concentration in the aminoacylation assay. The observed differences in the kinetic parameters for aminoacylation may be the consequence of the overall change in the enzyme conformation due to deletion of the positively charged C-terminal fragment, which can interact with other parts of the protein via electrostatic interactions. The involvement of the C-terminal peptide in the stabilization of the overall enzyme structure was further demonstrated by the comparison of the thermal stability of the full-length SerRS and the mutant SerRSC20. The proteins were first incubated at 42 °C. After various time intervals, aliquots were withdrawn and tested for aminoacylation activity. Much faster heat inactivation of truncated protein compared to the full-length SerRS was observed (Fig. 4), without significant degradation of the protein, as confirmed by Western blotting (not shown). This confirms that removal of the C-terminal fragment of SerRS strongly influences protein stability. The truncated protein may be folded into a less stable conformation and is therefore more accessible to the intracellular proteolytic apparatus, which would then not allow high level accumulation of SerRSC20 in the cell.




Figure 4: Heat inactivation curve of full-length (circle) and truncated (box) SerRS. An equal amount of protein was incubated at 42 °C in 50 mM Tris-HCl, pH 7.5, 2 mM DTT, 5% glycerol. At various time intervals, aliquots were withdrawn and used in the aminoacylation assay. The remaining serylation activity is shown; 100% activity corresponds to 31 and 29 pmol of charged tRNA for full-length and truncated enzyme, respectively. The concentration of serine in the assay was 1 mM.



C-terminal Truncation of SerRS Does Not Affect Its mRNA Level

This low level of overproduction of truncated SerRSC20 protein, raised the question of whether the transcriptional level of SES1C20 and the stability of its mRNA are altered. Thus, total RNA was isolated from yeast strain BR2727 transformed with either plasmid pVTU-103SES1 or pVTU-103SES1C20, which carry the structural gene SES1 and its truncated form, respectively, distal to the ADH promoter. Northern blot analysis showed that the truncation of 60 bp at the 3`-end of the SES1 coding region, does not influence mRNA production and/or its stability (Fig. 5). The multiple bands in lanes b and c (Fig. 5) may reflect the difference in the size of the transcripts originated from chromosomally encoded and plasmid-contained SES1 genes.


Figure 5: Northern blot analysis of total yeast RNA isolated from strain BR2727 transformed with pVTU-103 (a, 50 µg), pVTU-103SES1 (b, 25 µg), or pVTU-103SES1C20 (c, 25 µg). RNA was fractionated on a 1.5% agarose/formaldehyde gel and transferred to a Nytran membrane (Schleicher & Schuell). Hybridization to a 1.4-kb BamHI fragment of P-labeled yeast DNA, containing the SES1 gene, was at 42 °C, as was the final wash of the membrane.




DISCUSSION

Alignments of primary structures of a number of aminoacyl-tRNA synthetases have established that subunit sizes of eukaryotic enzymes are often larger than those of the corresponding enzymes from prokaryotic sources(28, 29) . These polypeptide chain extensions are found to be located at one extremity of the molecules, usually the N terminus, rather than as insertions within the conserved regions. The exceptions are eukaryotic isoleucyl- (30, 31) and seryl-tRNA synthetases, which carry the extensions at the C terminus. The common feature of N-terminal extensions of eukaryotic synthetases, is a high content of basic amino acids. However, there is no homology or common structural motif between these fragments. As regards the functional significance of N-terminal extensions, it was proposed earlier that this domain confers on the synthetase the ability to bind to polyanionic carriers, promoting the compartmentalization of these enzymes within the cytoplasm, through electrostatic interactions with as yet unidentified cellular components carrying negative charges(28, 32) . This hypothesis was experimentally tested on the aspartyl (33, 34, 35) , glutaminyl(36, 37) , lysyl(28, 38) , and methionyl (39) systems. It was demonstrated that truncated enzymes lose affinity for anionic carriers(28, 33, 37) . Although in some cases the extension, or part of it, was important for structure/activity of the enzyme, extensions were not found to be essential for the viability of the cells(36, 39) . These studies reinforce the concept that eukaryotic synthetases have quasi-independent domains not found in their prokaryotic counterparts, which may confer a function distinct from aminoacylation. Recent experiments show that the N-terminal extensions of mammalian aspartyl- and arginyl-tRNA synthetases are responsible for mediating its association with the multisynthetase complex(40, 41) , as also shown earlier for rat liver arginyl-tRNA synthetase(42) .

In the serine system, three eukaryotic seryl-tRNA synthetases, from S. cerevisiae(8) , Chinese hamster(9) , and human,^1 share sequence homology with their prokaryotic counterparts from E. coli(4) , T. thermophilus(5) , C. burnetii(7) , and B. subtilis(6) , except that they contain a C-terminal fragment of 20 or more mostly basic and hydrophilic amino acids (Fig. 1). Interestingly, the sequence of the first 25 residues(469-493) in the C-terminal extension in human SerRS is almost identical (with the exception of one amino acid) to the sequence of the corresponding peptide of Chinese hamster enzyme. Crystallographic studies have shown that two prokaryotic enzymes, isolated from E. coli and T. thermophilus fold to very similar conformations(5) . Since none of the eukaryotic seryl-tRNA synthetases has been crystallized yet, the influence of the C-terminal domain on the overall structure of the protein is unknown. Although the deletion of the C-terminal extension of the yeast enzyme is not essential for the viability of the cell, thermal stability of the truncated enzyme determined in vitro is much below that of the full-length form. The abundance of positively charged (lysines) and hydrophilic (serines) amino acids in the C-terminal peptides of eukaryotic enzymes (Fig. 1), makes it likely that this domain is involved in maintaining the overall structure of the enzyme via electrostatic interactions. While the truncated enzyme shows full activity in the aminoacylation reaction in the presence of elevated serine concentration, as shown by very similar specific activities and k values for SerRS and SerRSC20 (Table 3), its affinity for the substrates differs from the full-length protein. At 30 °C, the K(m) for tRNA is 3.6-fold reduced for truncated enzyme, while the K(m) for serine is 4-fold elevated. These relatively small differences in kinetic parameters could be explained by an overall change of protein conformation, rather than by the loss of direct contacts with substrate binding sites, which would be expected to lead to far more dramatic changes.


FOOTNOTES

*
This work was supported by grants from the National Institutes of Health, NIH/FIRCA, and UNIDO. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
To whom correspondence should be addressed: Dept. of Molecular Biophysics and Biochemistry, Yale University, P. O. Box 208114, 266 Whitney Ave., New Haven, CT 06520-8114. Tel.: 203-432-6200; Fax: 203-432-6202.

(^1)
M. Härtlein, personal communication.

(^2)
The abbreviations used are: SerRS, seryl-tRNA synthetase; SerRSC20, C-terminal 20 residue-truncated SerRS; DTT, dithiothreitol; PMSF, phenylmethylsulfonyl fluoride; PAGE, polyacrylamide gel electrophoresis; bp, base pair(s); kb, kilobase pair(s).

(^3)
I. Weygand-Durasevic, B. Lenhard, S. Filipic, and D. Söll, unpublished observation.


ACKNOWLEDGEMENTS

We are indebted to B. Rockmill for the help with gene disruption experiment and the gift of S. cerevisiae strains. We thank T. Vernet and D. Pridmore for the plasmids, F. Borel for useful advice concerning enzyme purification, and M. Härtlein for communicating the amino acid sequence of human SerRS prior to publication.


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