©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
Cytoplasmic and Extracellular IsK Peptides Activate Endogenous K and Cl Channels in Xenopus Oocytes
EVIDENCE FOR REGULATORY FUNCTION (*)

(Received for publication, November 1, 1995; and in revised form, December 26, 1995)

Iris Ben-Efraim (1) Yechiel Shai (1)(§) Bernard Attali (2)(¶)

From the  (1)Department of Membrane Research and Biophysics and the (2)Department of Neurobiology, The Weizmann Institute of Science, Rehovot, 76100 Israel

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

IsK is a 14.5-kDa type III membrane glycoprotein which induces slowly activating K and Cl currents when expressed in Xenopus oocytes and HEK 293 cells. Recently, mutagenesis experiments identified amino- and carboxyl-terminal domains of IsK as critical for induction of Cl and K currents, respectively. This hypothesis was tested by examining effects of synthetic IsK hydrophilic peptides on untreated Xenopus oocytes. In agreement with IsK membrane topology, we show here that peptides derived from carboxyl and amino termini are sufficient to activate slow K and Cl channels whose biophysical and pharmacological characteristics are similar to those exhibited by the native IsK protein. That data provide further evidence that IsK is a regulatory subunit of pre-existing silent channel complexes rather than a channel per se.


INTRODUCTION

The last few years have been an exciting time for our understanding of the molecular structure and diversity of voltage-gated K channels belonging to the Shaker-like superfamily (for review, see (1, 2, 3, 4, 5) ). Contrastingly, since its original cloning by expression in Xenopus oocytes, the nature of the IsK protein (or minK) remained a mystery (for review, see (6) and (7) ). From a structural point of view, IsK is an exception to the family of K channels. IsK is a 14.5-kDa type III glycoprotein with one transmembrane segment which has no sequence homology with other cloned functional channels(8) . It is a member of a family of small bitopic membrane proteins which induce upon expression in Xenopus oocytes slowly activating voltage-dependent currents(6, 7, 9) . This family includes phospholemman(10) , influenza virus M2 protein(11) , CHIF(12) , and Mat-8(13) . When expressed in Xenopus oocytes or in HEK 293 cells, the IsK protein evokes a unique slowly activating, voltage-dependent K-selective current that closely resembles the slow component I of the cardiac delayed rectifier(8, 14) . Two main hypotheses concerning the nature of this protein were raised. The first was that IsK alone is sufficient to form a voltage-gated K channel, because mutations in the transmembrane domain altered the gating of the K current expressed in Xenopus oocytes and changed the relative permeabilities of NH(4) and Csversus K(15, 16) . However, attempts to express IsK as a K channel in a variety of host cells and after lipid bilayer reconstitution have failed(17) . The second hypothesis was that IsK forms functional K channels by association with an endogenous oocyte factor or with pre-existing silent channels(18, 19, 20) . Furthermore, IsK mutagenesis suggested that the amino-terminal domain is critical for the induction of Cl currents while the carboxyl-terminal domain is critical for the activation of K channel activity(18) . These findings hinted at the possibility that IsK is a regulatory subunit of heteromultimeric channel complexes rather than a channel per se.

To test this hypothesis, synthetic IsK hydrophilic peptides were applied to untreated Xenopus oocytes. In agreement with IsK membrane topology, we show here that internal or external application of IsK peptides derived from the carboxyl- and amino-terminal domains are sufficient to activate slow K or Cl currents, respectively. The peptide-activated channels displayed characteristics similar to those exhibited by the native IsK protein, namely voltage dependence, activation kinetics, ion selectivity, and pharmacology. Our data provide clear evidence for the nature of IsK as a prototypic member of a family of short membrane transport proteins with regulatory function.


EXPERIMENTAL PROCEDURES

Peptide Synthesis and Purification

Peptides were synthesized by a solid-phase method on pyridine-2-aldoximine methyl-amino acid resin (0.15 meq)(21) , as described previously(22) . The peptides were subjected to amino acid analysis to confirm their composition.

Electrophysiology

Xenopus laevis were purchased from C.R.B.M. (Montpellier, France). Ovarian lobes were surgically removed as described (23) in a Ca-free OR2 solution (82.5 mM NaCl, 2 mM KCl, 1 mM MgCl(2), 5 mM HEPES (pH 7.5 with NaOH)). Oocytes were kept for 2-9 days in OR2 standard solution (82.5 mM NaCl, 2 mM KCl, 1 mM MgCl(2), 1.8 mM CaCl(2), 5 mM HEPES (pH 7.5 with NaOH)) supplemented with 100 IU/ml penicillin and 100 µg/ml streptomycin.

Command pulse protocols, data acquisition, and analyses were performed using pClamp software (Axon Instruments, Foster City, CA) as described (23) . Oocytes were perfused in OR2 standard solution, and all experiments were carried out with the oocyte membrane held at -80 mV or -60 mV. Oocytes were injected with full-length mouse IsK cRNA or full-length dog phospholemman (10 ng/oocyte), and the resulting currents were recorded 2 days following cRNA injection. For intracellular peptide injection, a volumetrically calibrated micropipette was used and peptide content in 20 nl was ejected by brief pressure pulses. The peptides were dissolved in 20 mM HEPES (pH 7.4), 120 mM KCl and were briefly sonicated before microinjection. To record depolarization-activated currents, 20-s depolarizing pulses were applied from a holding potential of -60 mV at 45-s intervals and 1 min after microinjecting or superfusing the peptides. For C-27-activated currents, the reversal potential determination was performed by changing sequentially the perfusate to a series of OR2 solutions in which Na was replaced by K. For extracellular peptide perfusion, the peptides were dissolved in OR2 superfusing solutions. To record hyperpolarization-activated currents, 5-s hyperpolarizing pulses were evoked from a holding potential of -10 mV at 1-min intervals and 1 min after superfusing or microinjecting the peptides. For N-34-activated currents, the reversal potential determination was accomplished by changing sequentially the perfusate to a series of OR2 solutions in which Cl was replaced by MES. (^1)Values from experiments with multiple data points are expressed as mean ± S.E.


RESULTS

Mutagenesis experiments have shown that an intracellular carboxyl-terminal domain of IsK is essential for the induction of the slow K current in Xenopus oocytes(18) . A 27-mer hydrophilic peptide (C-27), spanning a carboxyl-terminal region of the rat IsK sequence (positions 68-94) (22) and located immediately downstream from the transmembrane segment, was synthesized and microinjected into Xenopus oocytes (Fig. 1). Within 30 s after C-27 peptide injection, membrane depolarization above a threshold of -50 mV evoked a slowly activating voltage-dependent outward current (Fig. 2, A and C). This current was very similar to the slow K current produced by the native IsK. The depolarization-activated current could last for more than 20 min after injection and then declined to 0 after 30 min. Current amplitude was partly dependent on peptide concentration, being activated above 20 µM C-27, but then rapidly saturating above 100 µM C-27 (not shown). No currents could be evoked, when internally applied C-27 was tested upon hyperpolarization or when it was perfused in the external bath solution (n = 4, 3 batches). A 34-mer hydrophilic peptide(N-34), spanning an amino-terminal region of the rat IsK sequence (position 10-43)(22) , did not evoke any current when injected into the oocyte (at 200 µM; n = 5, 3 batches). Other non IsK peptides such as P1, P2, and P3 (Fig. 1) were ineffective at 200 µM (n = 5, 3 batches).


Figure 1: List of the rat IsK-derived and control peptides. Peptides were synthesized using the solid phase method and high performance liquid chromatography-purified as described under ``Experimental Procedures.''




Figure 2: Expression of slow K currents in Xenopus oocytes microinjected with C-27 IsK peptide. A, currents were evoked by 20-s depolarizing pulses from a holding potential of -60 mV to + 60 mV in 20-mV increments at 45-s intervals and 1 min after microinjecting 100 µM C-27. B, from a holding potential of -80 mV, tails of C-27-activated currents were recorded by a 20-s depolarizing prepulse to +20 mV and followed by test potentials between -30 mV and -130 mV in 20-mV decrements. C, current-voltage relationships for water-injected (open triangles), 10 ng of full-length IsK cRNA-injected (open circles), and 100 µM C-27-injected (filled circles) oocytes. The current amplitudes were measured at the end of a 20-s depolarizing pulse from -50 mV to +50 mV. D, K selectivity of C-27-induced currents measured by tail current reversal potentials (open circles). The straight line is the Nernst relationship for a perfectly selective K current. Points shown are the means ± S.E. of 4 independent experiments.



It was difficult to describe accurately the activation kinetics of C-27-activated currents, since it did not reach steady-state even after several minutes (at + 40 mV, 6-12 s, by fitting the 20-s pulse). Unlike deactivation kinetics, the activation rates varied considerably among oocytes. Tail current deactivation was faster than activation kinetics, requiring seconds for full relaxation (Fig. 2B), and was fit as a biexponential decay (at -80 mV repolarization, = 0.27 ± 0.08 s and = 1.80 ± 0.25 s; n = 7; 3 batches). The current was selective for K ions. The slope of the tail current reversal potentials at various [K](o) (substituted for Na) was 52.6 mV per decade, consistent with a K selective current (Fig. 2D; n = 4, in 2 batches). In Xenopus oocytes, voltage steps to potentials above -20 mV usually give rise to transient Ca-activated outward Cl currents in OR2 standard recording solutions. To examine a possible interference of outward Cl conductance, we studied the C-27-activated outward currents under Cl-free recording solutions (Cl substituted with gluconate). Under these conditions, Cl influx was prevented and no Ca-activated Cl currents could be recorded in the presence of the Ca ionophore A23187 (1 µM) at voltage steps from -20 mV up to 60 mV (not shown). In gluconate recording solutions, intracellularly applied C-27 (100 µM) was able to evoke slowly activating K-selective currents upon step depolarization to 40 mV (Fig. 4A, control trace). Although the activation was slightly faster than in Cl-containing solutions, the pharmacology was nearly identical with that found with the expression of the native IsK protein. Fig. 4A shows that barium (5 mM) and clofilium (100 µM) blocked the C-27-induced K current by 70 ± 9% and 60 ± 10%, respectively (n = 5; 3 different batches). Tetraethylammonium (30 mM) caused a 50 ± 11% blockade of the current while lanthanum at 100 µM was ineffective (n = 5, 3 batches). The lack of lanthanum blockade is in contrast with a previous report (24) and suggests that other domains of IsK are necessary to obtain an effective inhibition. Similar results were obtained with aspartate or methanesulfonate recording solutions (not shown).


Figure 4: Pharmacology of C-27- and N-34-activated currents. A, effects of clofilium (100 µM) and Ba (5 mM) on K currents activated by 100 µM C-27, following a 20-s depolarizing pulse to +40 mV (1 min after C-27 microinjection). B, effects of Ba (1 mM) and DIDS (1 mM) on Cl currents activated by 50 µM N-34, following a 5-s hyperpolarizing pulse to -150 mV (1 min after starting peptide superfusion; holding potential -10 mV). C, ability of N-20 (200 µM) and N-13 (10 µM) to induce hyperpolarization-activated currents as in B.



The role of the amino-terminal domain of IsK in activating slow Cl currents was examined by applying extracellularly N-34 (Fig. 1) to untreated oocytes. Upon hyperpolarization, a slowly developing inward current was evoked above a threshold of -90 mV (Fig. 3). This current was very similar to that induced by phospholemman or by IsK (at high cRNA concentration)(10, 18) . Since some batches of untreated oocytes did express a similar slow inward current, we always tested the effects of the peptides in oocytes that did not exhibit this endogenous inward current. The N-34 action occurred within 15 s and could be readily reversed upon washing out of the peptide, suggesting that the peptide only weakly associates with the endogenous oocyte channel. Like C-27 and above a threshold of 30 µM, current amplitude was not strictly dependent on peptide concentration and saturated above 100 µM (not shown). This lack of linear concentration dependence suggests that the peptide binding to the oocyte channels does not follow a simple bimolecular reaction. N-34-induced current activated slowly with a sigmoidal waveform and failed to reach steady state within 1 min, as described previously for phospholemman(10) . Tail currents deactivated within less than 10 s at -10 mV, while the current relaxation process was much slower at -40 mV (Fig. 3B). No currents were evoked after depolarization or if N-34 (at 100 µM final concentration) was injected intracellularly. C-27, P1, P2, and P3 (Fig. 1) were ineffective at 200 µM (n = 4, 3 batches).


Figure 3: Expression of slow Cl currents in Xenopus oocytes perfused with N-34 amino-terminal IsK peptide. A, current records were evoked by 5-s hyperpolarizing pulses from a holding potential of -10 mV between -90 mV to -170 mV in 20-mV decrements at 1-min intervals and 1 min after superfusing 50 µM N-34. B, from a holding potential of -10 mV, tails of N-34-induced currents were studied using a 5-s hyperpolarizing prepulse to -150 mV and followed by test potentials between -40 mV and +20 mV in 10-mV increments at 1-min intervals. C, current-voltage relationships for water-injected (open triangles), 10 ng of full-length phospholemman cRNA-injected (open circles), and 50 µM N-34-perfused (filled circles) oocytes. The current amplitudes were measured at the end of a 5-s hyperpolarizing pulses from 0 mV to -200 mV. D, Cl selectivity of N-34-induced currents measured by the tail current reversal potentials (open circles). The straight line is the Nernst relationship for a perfectly selective Cl current with a [Cl] = 65 mM. Points shown are the means ± S.E. of 3 independent experiments.



To determine the ionic selectivity underlying the N-34-induced inward current, we measured the dependence of the tail current reversal potential on [Cl](o) (Fig. 3D). As expected for Cl channels, the currents reversed direction at about -25 mV in standard solutions, and the reversal potential shifted to more positive values, when [Cl](o) decreased (Fig. 3D). However, the deviation of the curve from the Nernst relation, especially at low [Cl](o), suggests that other conductances may also be involved. This feature is reminiscent of that found for phospholemman-induced slow inward currents (10) and for slow endogenous oocyte channels I

In order to narrow down more precisely the active domain of the IsK amino-terminal, we looked at the effects of two externally applied peptides (Fig. 1). N-20, which overlaps with the proximal portion of N-34, was inactive (up to 200 µM; n = 3, 2 batches) ( Fig. 1and Fig. 4C). By contrast, N-13 (amino acids 31-43) was active and reached its maximal effect at 10 µM ( Fig. 1and Fig. 4C; n = 3, 2 batches). Thus, this short domain suffices by itself to act on the channel complex.


DISCUSSION

In this report we have shown that intracellular or extracellular application of specific hydrophilic IsK peptides was sufficient to mimic the native IsK induction of slow K and Cl currents(6, 7, 8, 18) . It is clear that these peptides could not form by themselves ion-conducting pores, since they do not interact with the membrane and their action is not irreversible. The peptide-induced channel activity is specific since non-IsK peptides were ineffective. The specificity of the peptide interaction is further evidenced by the ability of intracellular carboxyl terminus and extracellular amino terminus peptides to exclusively evoke K or Cl currents, respectively. This is in agreement with the IsK membrane topology. Our data strongly support the notion that IsK must associate with some endogenous oocyte component to form a functional channel complex(18, 19, 20) . However, mutations in the transmembrane domain of IsK were found to alter channel selectivity, open channel block, and gating kinetics(15, 16) . To explain this apparent controversy, we suggest that IsK, acting as a regulatory subunit, has some contribution in defining the K pore properties and in modulating channel gating. This is actually the case with the beta1 subunit of voltage-dependent Na channels (same membrane topology as IsK) and voltage-dependent Ca channels which alter the voltage dependence of channel gating(26) .

It has been suggested recently that IsK might become functional by interacting with either a rare lipid, a cytoskeletal protein, or a channel protein subunit(19) . Regarding our data, we favor the latter proposal for two main reasons. First, it is energetically unfavorable for a hydrophilic peptide to associate with a lipid to form a functional channel. Second, it seems unlikely that an extracellularly applied peptide such as N-34 will interact with an intracellular cytoskeletal component to produce a current. Naturally, it does not exclude that the whole channel complex could be linked to the cytoskeleton. Thus, the reasonable explanation is that the IsK peptides interact with endogenous oocyte channels to activate them. In such a model, IsK may act at least in one of the two ways. 1) It could function as an activator or a regulatory subunit which activates pre-existing silent channels by direct protein-protein interactions. 2) Alternatively, IsK could act by recruiting inactive channels and functions as a chaperone that facilitates assembly of multimeric channel complexes. However, the relatively fast peptide action together with the ability of externally applied N-34 or N-13 to evoke slow Cl currents are not compatible with this view.

The oligomeric nature of the IsK channel complex remains unknown; however, recent studies suggested that it could be made of just two IsK monomers associated with as yet unidentified non-IsK subunits(20) . The very slow gating kinetics of IsK suggest that it could activate by a unique mechanism. A model in which IsK channels activate by voltage-dependent subunit aggregation has been proposed (27) . Cross-linking or Hg-induced chelation of IsK subunits were found to hold the channel complex in an open conducting state(27, 28) . This mechanism of subunit aggregation also accommodates our view of a heteromultimeric subunit oligomerization process.

It is clear that the slow K and Cl currents are not specifically and exclusively induced by the IsK protein in Xenopus oocytes. Other structurally similar small bitopic membrane proteins like CHIF, Mat-8, or phospholemman are also able to activate these slowly activating currents(10, 12, 13) . For example, IsK and CHIF evoke slow K currents which fail to reach steady state within tens of seconds and are sensitive to block by Ba and clofilium. Since these proteins share no sequence similarity, it suggests that the specificity requirements for binding to the endogenous channel complex are relatively low. The conformation that the peptides adopt upon binding is unknown; however, the various peptidic motifs must share some yet undefined structural or conformational similarities. The transient character of the peptide action suggests that the transmembrane domain and may be other regions such as the extreme amino terminus may help in stabilizing the physical interaction between IsK and other subunits of the channel complex, independently of the gating process.

In conclusion, our findings provide further evidence for the nature of IsK as a member of a family of short bitopic membrane proteins which are capable of activating endogenous and otherwise silent ion channels. It is now crucial to investigate which physiological roles subserve IsK-channel protein interactions in epithelia, T lymphocytes, or cardiac cells and what are the molecular structures of these interacting channel proteins.


FOOTNOTES

*
This study was supported in part by research grants from Leukemia Research (Chicago) and the James E. Roundstein Foundation (Hotpoint, Alaska) (to B. A., a recipient of an Israel Cancer Research Fund career development award) and from the Basic Research Foundation administered by the Israel Academy of Sciences and Humanities and the MINERVA Foundation, Munich, Germany (to Y. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence may be addressed. Tel.: 972-8-342-315; Fax: 972-8-344-131; bnattali{at}weizmann.weizmann.ac.il.

§
To whom correspondence may be addressed. Tel.: 972-8-342-711; Fax: 972-8-344-112; bmshai{at}weizmann.weizmann.ac.il.

(^1)
The abbreviations used are: MES, 2-(N-morpholino)ethanesulfonic acid; DIDS, 4,4`-diisothiocyanostilbene-2,2`-disulfonic acid; SITS, 4-acetamido-4`-isothiocyanostilbene-2,2`-disulfonic acid.


ACKNOWLEDGEMENTS

We are grateful to Prof. Vivian Teichberg for helpful discussions and for use of his voltage clamp setup and to Prof. Steve Karlish and Dr. Nava Moran for critical reading of the manuscript.


REFERENCES

  1. Miller, C. (1991) Science 252, 1092-1096 [Medline] [Order article via Infotrieve]
  2. Salkoff, L., Baker, K., Butler, A., Covarrubias, M., Pak, M. D., and Wei, A. (1992) Trends Neurosci. 15, 161-166 [CrossRef][Medline] [Order article via Infotrieve]
  3. Pongs, O. (1992) Physiol. Rev. 72, S69-S88
  4. Hoshi, T., and Zagotta, W. N. (1993) Curr. Opin. Neurobiol. 3, 283-290 [Medline] [Order article via Infotrieve]
  5. Pongs, O. (1995) Semin. Neurosci. 7, 137-146 [CrossRef]
  6. Kaczmarek, L. K. (1991) New Biol. 3, 315-323 [Medline] [Order article via Infotrieve]
  7. Swanson, R., Hice, R. E., Folander, K., and Sanguinetti, M. C. (1993) Semin. Neurosci. 5, 117-124
  8. Takumi, T., Ohkubo, H., and Nakanishi, S. (1988) Science 242, 1042-1045 [Medline] [Order article via Infotrieve]
  9. Blumenthal, E. M., and Kaczmarek, L. K. (1992) Neurochem. Res. 17, 869-876 [Medline] [Order article via Infotrieve]
  10. Moorman, J. R., Palmer, C. J., John, J., III, Durieux, M. E., and Jones, L. R. (1992) J. Biol. Chem. 267, 14551-14554 [Abstract/Free Full Text]
  11. Pinto, L. H., Holsinger, L. J., and Lamb, R. A. (1992) Cell 69, 517-528 [Medline] [Order article via Infotrieve]
  12. Attali, B., Latter, H., Rachamim, N., and Garty, H. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 6092-6096 [Abstract/Free Full Text]
  13. Morrison, B. W., Moorman, J. R., Kowdley, G. C., Kobayashi, Y. M., Jones, L. R., and Leder, P. (1995) J. Biol. Chem. 270, 2176-2182 [Abstract/Free Full Text]
  14. Freeman, L. C., and Kass, R. S. (1993) Circ. Res. 73, 958-973
  15. Takumi, T., Moriyoshi, K., Aramori, I., Ishii, T., Oiki, S., Okada, Y., Ohkubo, H., and Nakanishi, S. (1991) J. Biol. Chem. 266, 22192-22198 [Abstract/Free Full Text]
  16. Goldstein, S. A. N., and Miller, C. (1991) Neuron 7, 403-408 [CrossRef][Medline] [Order article via Infotrieve]
  17. Lesage, F., Attali, B., Lakey, J., Honore, E., Romey, G., Faurobert, E., Lazdunski, M., and Barhanin, J. (1993) Receptors Channels 1, 143-152 [Medline] [Order article via Infotrieve]
  18. Attali, B., Guillemare, E., Lesage, F., Honore, E., Romey, G., Lazdunski, M., and Barhanin, J. (1993) Nature 365, 850-852 [CrossRef][Medline] [Order article via Infotrieve]
  19. Blumenthal, E. M., and Kaczmarek, L. K. (1994) J. Neurosci. 14, 3097-3105 [Abstract]
  20. Wang, K.-W., and Goldstein, S. A. N. (1995) Neuron 14, 1303-1309 [Medline] [Order article via Infotrieve]
  21. Shai, Y., Bach, D., and Yanovsky, A. (1990) J. Biol. Chem. 265, 20202-20209 [Abstract/Free Full Text]
  22. Ben-Efraim, I., Strahilevitz, J., Bach, D., and Shai, Y. (1994) Biochemistry 33, 6966-6973 [Medline] [Order article via Infotrieve]
  23. Honore, E., Attali, B., Romey, G., Heurteaux, C., Ricard, P., Lesage, F., Lazdunski, M., and Barhanin, J. (1991) EMBO J. 10, 2805-2811 [Abstract]
  24. Hice, R. E., Folander, K., Salata, J. J., Sanguinetti, M. C., and Swanson, R. (1994) Pfluegers Arch. Eur. J. Physiol. 426, 139-145 [Medline] [Order article via Infotrieve]
  25. Kowdley, G. C., Ackerman, S. J., John, J., III, Jones, L. R., and Moorman, J. R. (1994) J. Gen. Physiol. 103, 217-230 [Abstract]
  26. Isom, L. L., De Jongh, K. S., and Catterall, W. A. (1994) Neuron 12, 1183-1194 [Medline] [Order article via Infotrieve]
  27. Varnum, M. D., Maylie, J., Busch, A., and Adelman, J. P. (1995) Neuron 14, 407-412 [Medline] [Order article via Infotrieve]
  28. Busch, A. E., Waldegger, S., Herzer, T., Raber, G., Gulbins, E., Takumi, T., Moriyoshi, K., Nakanishi, S., and Lang, F. (1995) J. Biol. Chem. 270, 3638-3641 [Abstract/Free Full Text]

©1996 by The American Society for Biochemistry and Molecular Biology, Inc.