©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
Different Glycosylation Requirements for the Synthesis of Enzymatically Active Angiotensin-converting Enzyme in Mammalian Cells and Yeast (*)

(Received for publication, November 1, 1995; and in revised form, January 2, 1996)

Ramkrishna Sadhukhan Indira Sen (§)

From the Department of Molecular Cardiology, Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

For facilitating crystallization and structural studies of the testicular isozyme of angiotensin-converting enzyme (ACE(T)), we attempted the production of enzymatically active ACE(T) proteins which are unglycosylated or underglycosylated. Expression in Escherichia coli of the rabbit ACE(T) cDNA resulted in the synthesis of an unglycosylated but inactive protein. Similarly, unglycosylated ACE(T) synthesized in HeLa cells, by using a cDNA in which all five potential N-glycosylation sites had been mutated, was inactive and rapidly degraded. Several ACE(T) variants carrying mutations in one or more of the potential N-glycosylation sites were used to examine the role of glycosylation at specific sites on ACE(T) synthesis, transport to the cell surface, cleavage processing, and enzyme activity. These experiments demonstrated that allowing glycosylation only at the first or the second site, as counted from the NH(2) terminus, was sufficient for normal synthesis and processing of active ACE(T). In contrast, ACE(T)g3, which had only the third glycosylation site available, was unglycosylated, enzymatically inactive and rapidly degraded. N-Glycosylated ACE(T) could also be produced in yeast. Surprisingly, the mutant ACE(T)g3 was synthesized, N-glycosylated, and properly transported in yeast. Wild type and mutant ACE proteins were cleavage-secreted from yeast and enzymatically active.


INTRODUCTION

Angiotensin-converting enzyme (ACE) (^1)(EC 3.4.15.1, dipeptidyl carboxypeptidase) plays a major role in blood pressure regulation and fluid and electrolyte homeostasis by acting on two major vasoactive peptides. It converts the inactive precursor angiotensin I to an active vasopressor peptide angiotensin II and inactivates the vasodepressor peptide bradykinin(1, 2, 3) . ACE has two structurally related isozymic forms (4, 5, 6) encoded by two different mRNAs, which arise from the same gene by tissue-specific choice of the alternative transcription initiation sites(7, 8, 9, 10, 11, 12, 13) . In rabbit, the smaller isozyme, testicular (T) ACE has 737 amino acid residues and the larger pulmonary (P) isozyme has 1309 residues(7, 9) . The COOH-terminal 665 residues of the isozymes are identical, whereas their NH(2) termini are unique. They carry signal sequences at their NH(2) termini which are removed during their biosynthesis. Both isozymes are extensively glycosylated and expressed as a cell surface Type 1 ectoprotein anchored in the plasma membrane by a 17-residue-long hydrophobic transmembrane domain near the COOH terminus. There is ample evidence to suggest that the COOH-terminal 30 residues constitutes the cytoplasmic tail and the rest of the protein is extracellular. In tissue culture(14, 15, 16, 17, 18) , as well as in vivo, the extracellular domain of ACE is released into the culture media or in body fluids, by a regulated proteolytic cleavage of the membrane anchoring domain. Indeed, a COOH-terminally truncated, soluble form of enzymatically active ACE is found in many body fluids, including serum(16, 18, 19) . Thus, several important post-translational modifications occur during biosynthesis of ACE.

Rabbit ACE(T) has five potential N-glycosylation sites as indicated by the presence of the Asn-X-Ser/Thr motif in its primary structure. In addition, it has a cluster of threonine residues near the amino terminus that can potentially be O-glycosylated(7) . ACE(T) isolated from tissues and transfected cell lines is heavily glycosylated carrying both N- and O-linked sugars. Using inhibitors of glycosylation and a mutant cell line defective in protein glycosylation, we have shown that complete blockage of glycosylation causes rapid intracellular degradation of ACE(T). However, ACE(T) synthesized without N-linked complex sugars and O-linked sugars is transported to the cell surface, cleavage-secreted, and enzymatically active(20) . Similarly, Ehlers et al.(21) have shown that a mutant ACE(T) devoid of most of its O-linked sugars has enzymatic activity.

In our current study, we evaluated the contributions of each of the five potential N-glycosylation sites of ACE(T) toward its synthesis, glycosylation, intracellular transport, cleavage secretion, and enzymatic activity. This was accomplished by site-directed mutagenesis of these sites individually or in combinations. Our studies demonstrated interesting differences among the contributions made by the different sites. Moreover, we attempted the production of wild type and mutant ACE(T) proteins in bacteria and yeast so that large quantities of these proteins can be easily produced for structural studies. In the process, we revealed an unexpected difference between yeasts and mammalian cells in the utilization of a specific glycosylation site in ACE(T).


EXPERIMENTAL PROCEDURES

Materials

Pichia pastoris GS115(His-4) was used for expression of ACE(T) and its mutants (the Pichia expression kit, Invitrogen Corp., San Diego, CA). Escherichia coli TOPIOF was used for all plasmid construction and propagation. The Muta-Gene phagemid in vitro mutagenesis kit and fluorescein-labeled anti-goat IgG were obtained from Bio-Rad and Vector Laboratories, respectively.

Expression of ACE(T) in E. coli

The plasmid pGEM9T-ACE(T)(20) and the two primers 5`CCGGAAT2TCGTCGACCCATATGGTAACAGTCAACCAGGGG-3` (sense) and 5`-CTGCAGGTCCTGAACCTCCTTTATGATGCGCTT-3` (antisense) were used in a polymerase chain reaction to amplify a portion of ACE gene (113-486 base pairs). The products were digested with EcoRI and BstEII and ligated to a EcoRI/BstEII-digested pGEM9T-EACE(20) . The positive clone was identified by restriction analysis and further confirmed by sequencing the entire insert. The resulting construct, pGEM9T-EACE34N, was digested with NdeI and BamHI, ligated to NdeI/BamHI-digested pET3a, a high expression bacterial vector (Novagen, Madison, WI), and expressed in E. coli BL21 (DE3). The construct pET3a-ACE(T) will encode for a smaller ACE protein, lacking the 34 NH(2)-terminal residues, not required for expression in E. coli and 63 COOH-terminal residues not essential for enzymatic activity or secretion(14) .

Generation of Site-specific Mutants

To eliminate the consensus sites for N-linked glycosylation in the wild type ACE protein, oligonucleotide-directed mutagenesis was used (22, 23) to substitute the Asn codon for Gln codon at each of the sites containing the consensus sequence (Asn-X-Ser/Thr) for N-linked glycosylation. The five potential N-linked glycosylation sites are designated g1, g2, g3, g4, and g5 corresponding to Asn found at ACE(T) residues 108, 125, 145, 373, and 622, respectively (Table 1). ACE cDNA cloned in pTZ18U vector was the starting material for mutagenesis, which was carried out by using single-stranded cDNA cloned in M13 vectors. The following mutant antisense oligonucleotides were used for mutating the five sites: g1, 5`-GTGGTGATCTGGGTGTTATAG-3`; g2, 5`-GAGGGTGTGCTGGGCTATCTG-3`; g3, 5`-CTGGTGGCCTGCTGGAAGTTG-3`; g4, 5`-CATCGACTTCTGCCAGAACTC-3`; g5, 5`-GGCTGACATCTGGGGCTGGCC-3`. A cDNA-containing mutation at one site served as a template for further mutagenesis and so on until all constructs were generated. The nucleotide sequence at the site of mutation was confirmed by sequencing to ensure that only the desired mutation has been created. A total of 19 N-linked glycosylation mutants were constructed, each designated ACE(T)g(n), where n is a set of numbers defining the N-linked glycosylation sites that are available: e.g. wild type ACE(T) is ACE(T)g12345, and a mutant lacking the g2 site is ACE(T) g1345 and a mutant lacking all five sites is ACE(T)g0 (see Table 1).



Expression of ACE(T) and Its Mutants in Yeast

Vector Construction and Transformation

P. pastoris, a methylotropic yeast host, was chosen for this study, since its glycosylation pattern resembles more closely that of mammalian cells(24) . Expression vector pHIL-S1 (Invitrogen Corp.) containing methanol-inducible alcohol oxidase (AOX1) promoter and PHO1 signal sequence was used to express ACE(T) and its various mutants. Briefly, a 2.4-kilobase EcoRI fragment containing the full coding sequence of ACE(T) was excised from the parent plasmid (pTZ18U-ACE(T)) and ligated to a EcoRI cleaved pHIL-S1 vector plasmid. Proper orientation of the insert was analyzed by restriction analysis using SfiI and XhoI. The resulting recombinant plasmid pHIL-S1-ACE(T)WT codes for a fusion protein containing signal sequences from both PHO1 gene and ACE(T). The recombinant plasmid pHIL-S1-ACE(T)WT was linearized with BglII and later transferred into Pichia yeast host through lithium acetate transfortmatiom method(25) . Several transformants were cultured and screened for ACE expression. The purified recombinant deglycosylated ACE(T) from P. pastoris migrated on SDS-PAGE at 70 kDa (Fig. 8). Hence, it is expected that both the signal sequences (PHO1 and ACE(T)) were cleaved, and the resulting mature ACE(T) is a nonfusion protein and resembles the native enzyme from mammalian cells. Amino-terminal sequencing is in progress to further confirm the cleaveage of both the signal sequences. Similarly three other constructs, pHIL-S1-ACE(T)g2, pHIL-S1-ACE(T)g3, and pHIL-S1-ACE(T)g13 with the coding sequences of the respective glycosylation mutants cloned in the vector were also generated, introduced in the genome of P. pastoris, and expressed.


Figure 8: Glycosylation status of ACE(T) produced in P. pastoris. Culture media from P. pastoris expressing ACE(T)WT (lanes 1-4) or ACE(T)g3 (lanes 5-8) were immunoprecipitated with anti-ACE antibody 2 days after methanol induction. The immunoprecipitates were boiled with SDS and left untreated(-) or treated with (+) deglycosylating enzymes as indicated.



Yeast Media, Growth, and Expression of ACE(T) Protein

Cells were grown in 100 ml of BMGY medium (yeast extract, 10 g; peptone, 20 g; glycerol, 10 ml; biotin, 400 µg; yeast nitrogen base with ammonium sulfate, 13.4 g; and 100 ml of 1 M potassium phosphate buffer, pH 6.0, per liter) at 30° C. After 2 days, cells were harvested, resuspended in BMMY medium (same as BMGY media with the exception that 5 ml of methanol/liter was added in place of glycerol) and incubated at 30° C for another 2 days to induce expression. Finally, the cultures were centrifuged, and the supernatant containing the secreted ACE(T) proteins were concentrated, extensively dialyzed against 20 mM Hepes buffer, pH 7.0, containing 300 mM NaCl and 50 µM ZnCl(2), and analyzed by Western blot analysis using anti-ACE antibody to detect the presence of ACE(T) proteins as well as assayed for enzymatic activity.

Transient Expression of ACE(T) Proteins, Pulse-Chase Analysis, Immunoprecipitation, and Deglycosylation

ACE(T) proteins were expressed in HeLa cells using the vaccinia virus-T7 RNA polymerase system as described earlier(20) . The transfected cells were pulse-labeled with [S]methionine for 30 min, and the label was chased for the indicated time. ACE-related proteins were immunoprecipitated from the cell extracts and media and analyzed by SDS-PAGE. For deglycosylation, immunoprecipitates were boiled with SDS as described earlier (20) and treated sequentially with 400 milliunits of N-glycosidase F, 10 milliunits of neuraminidase, and 1 milliunit of O-glycosidase for total deglycosylation or with one or a combination of the enzymes as indicated in the figures.

Western Analysis and Enzyme Activity Measurements

Western analysis was carried out using either anti-rabbit lung ACE (anti-ACE) or anti-COOH-terminal peptide (antipeptide) antibody (23) . ACE-enzyme activity was measured (23) using hippuryl-L-histidyl-L-leucine (Hip-His-Leu) as substrate.

Immunodetection of ACE(T) in Transfected HeLa Cells by Indirect Immunofluorescence

HeLa cells were grown on glass coverslips and transfected with ACE(T)WT or ACE(T)g3 cDNA as described. For labeling cell surface ACE(T), the transfected cells were fixed with 3.7% paraformaldehyde for 10 min at room temperature. To localize ACE(T) proteins in the intracellular compartment, the paraformaldehyde-fixed cells were permeabilized by treatment in 0.25% Triton X-100 for 5 min. After washing with 500 mM NaCl, 20 mM Tris-HCl, pH 7.5 (Tris-buffered saline), containing 2% fetal bovine serum, cells were incubated sequentially with anti-ACE antibody (1:3000) and fluorescein conjugated anti-goat IgG (1:100) for 30 min each, mounted, and photographed (Nikon Optiphot-2). Tris-buffered saline was used to wash cells in between each step.


RESULTS

Expression of ACE(T) in E. coli

We have observed previously that unglycosylated ACE(T), synthesized in HeLa cells in the presence of tunicamycin, is rapidly degraded intracellularly(20) . Since protein glycosylation does not occur in E. coli, we attempted the production of ACE(T) in bacteria as an alternative source of unglycosylated ACE. E. coli transformed with pET3a-ACE(T), but not with pET3a alone, produced a 70-kDa protein upon induction with isopropylthiogalactopyranoside. This protein had a molecular weight similar to that predicted for unglycosylated truncated ACE(T), and it reacted specifically with ACE(T) antibody (Fig. 1). Thus, unglycosylated ACE(T) could be produced in E. coli in ample quantities. This protein, however, was enzymatically inactive (data not shown). Attempts to denature and renature this presumably misfolded protein failed to produce an active enzyme. Thus, like many other biologically active mammalian glycoproteins, bacterially expressed ACE(T) is inactive.


Figure 1: Expression of ACE(T) in E. coli. Total cellular protein isolated from E. coli BL21(DE3) containing either pET3a vector alone (Control) or pET3a-ACE(T) clone was analyzed by SDS-PAGE. The proteins were transferred onto a nitrocellulose membrane and subjected to Western blot analysis with anti-ACE-antibody. The arrow indicates the expressed ACE(T).



Expression of Underglycosylated ACE(T) in HeLa Cells

Since we failed to produce enzymatically active unglycosylated ACE(T) in either HeLa cells or E. coli, we turned our attention to producing underglycosylated ACE(T) in mammalian cells. For this purpose, glycosylation at one or more of the five potential N-glycosylation sites, present in ACE(T) at residues 108, 126, 145, 373, and 622, was prevented. This was achieved by mutating the Asn residues, present at these sites, to Gln. Such a mutation does not alter the local net charge or hydrophobicity but prevents glycosylation. A panel of 19 ACE(T) mutants carrying various combinations of the five glycosylation site mutations was generated (Table 1). These mutants were named by indicating the sites, as counted from the amino terminus, still available for N-glycosylation. Thus, the mutant ACE(T)g2345 had only the first site mutated. The mutant proteins were expressed in HeLa cells and their synthesis, modification, processing, and cleavage secretion to the culture medium were monitored using published procedures(17, 20, 23) . As expected, ACE(T)g0, which had all five potential sites mutated, produced a 76-kDa protein similar to the one produced by ACE(T) WT in the presence of tunicamycin (Fig. 2). This protein was rapidly degraded intracellularly, and no ACE(T)g0 was secreted to the medium. The 76-kDa ACE(T)g0 protein was unglycosylated, as judged by endo-beta-N-acetylglucosaminidase H digestion and enzymatically inactive (data not shown). In the next series of experiments, synthesis and processing of ACE(T) mutants with four, three, or two available N-glycosylation sites were analyzed (Fig. 3, A-C). All of these mutants were synthesized, glycosylated, and secreted to the medium. Among the mutants carrying single mutations, only ACE(T)g1234 had a molecular weight similar to that of ACE(T)WT; others had lower molecular weights (Fig. 3A). This result suggests that ACE(T)WT is probably minimally glycosylated at site 5, but all other sites are used. For all mutants, like the wild type protein, the secreted form was slightly smaller than the cell-bound forms. Among the double mutants (Fig. 3B), there were noticeable differences with regards to the molecular weight, the rate of synthesis, and the rate of secretion. For example, ACE(T)g234 (lane 4, Fig. 3B) was synthesized much less than ACE(T)g124 (lane 2, Fig. 3B). ACE(T)g123 (lane 6) had the lowest molecular weight, and the cleavage secretion was much more efficient for ACE(T)g134 (lane 5) than for ACE(T)g135 (lane 3). Three triple mutants were tested (Fig. 3C). All of them were synthesized and cleavage-secreted normally. Taken together, these results suggest that the specific carbohydrate structures at different glycosylation sites in ACE(T) may be different, thus the different molecular weights of different species. They also suggest that glycosylation at different sites differentially affect the rate of synthesis and the rate of cleavage secretion of the corresponding protein.


Figure 2: Expression of unglycosylated ACE(T) mutants in HeLa cells. The figure shows synthesis, intracellular processing, and secretion of wild type ACE(T) (ACEWT) and the mutant ACE(T)g0 in HeLa cells. HeLa cells, infected with the recombinant vaccinia virus expressing T7 RNA polymerase, were transiently transfected with ACE cDNA (left and middle panels) and ACE(T)g0 cDNA (right panel). The cells were pulse-labeled with [S]methionine for 30 min followed by incubation without the labels for different periods of time as indicated by Chase (h). Labeled detergent lysates of cells (C) and medium (M) were immunoprecipitated and analyzed by SDS-PAGE. The middle panel had 5 µg/ml tunicamycin present in the culture medium at all times. Positions of molecular mass markers in kilodaltons are shown on the left.




Figure 3: Expression of underglycosylated ACE(T) mutants. HeLa cells were transfected with wild type or mutant ACE(T) cDNAs, pulse-labeled with [S]methionine, and the label was chased for 15 h. Detergent lysates of cells (C) and the culture medium (M) were immunoprecipitated and analyzed. A, single-site mutants. B, double-site mutants. C, triple-site mutants.



Expression of ACE(T) Mutants with Single Available N-glycosylation Sites

Since ACE(T)g12, ACE(T)g13, and ACE(T)g23 proteins were normally synthesized and secreted (Fig. 3C), we decided to examine the corresponding quadruple mutants from this set. ACE(T)g1, ACE(T)g2, and ACE(T)g3 had, respectively, only site 1, 2, or 3 available for glycosylation. ACE(T)g1 gave rise to a mature protein of 98 kDa (Fig. 4A, lane 4 and Table 2), which was cleavage-secreted normally (Fig. 4A, lane 8). ACE(T)g2 was slightly bigger and processed normally (Fig. 4A, lanes 2 and 6; Table 2). In contrast, we failed to detect any cell-bound or secreted ACE(T)g3 in this experiment (Fig. 4A, lanes 3 and 7). To explore this further, a detailed kinetic analysis of the synthesis and processing of these proteins was carried out in the experiment shown in Fig. 4B. ACE(T)WT, ACE(T)g2, and ACE(T)g1 had identical kinetics of synthesis and maturation. The conversion of the partially glycosylated form to the fully mature form was virtually completed within 4 h for all three proteins. ACE(T)g3 was synthesized in lower quantities. The protein had a molecular mass of 76 kDa (Table 2), and it was never converted to the high molecular weight form. Moreover, like ACE(T)g0 (Fig. 2), ACE(T)g3 was rapidly degraded intracellularly.


Figure 4: Expression of ACE(T) mutants with single N-glycosylation sites. A, HeLa cells were transfected with wild type or mutant cDNAs, and pulse-chase (15 h) analysis was performed as described in the legend of Fig. 3. Immunoprecipitated cell lysates (C) and media (M) from cells transfected with ACE(T)WT (lanes 1 and 5), ACE(T)g2 (lanes 2 and 6), ACE(T)g3 (lanes 3 and 7), and ACE(T)g1 (lanes 4 and 8) were analyzed. B, kinetics of biosynthesis: transfected HeLa cells were pulse-labeled and chased for 0, 2, 4, 8, and 15 h as indicated. Immunoprecipitated lysates of cells transfected with ACE(T)WT (lanes 1-5), ACE(T)g2 (lanes 6-10), ACE(T)g1 (lanes 11-15), and ACE(T)g3 (lanes 16-20) cDNA were analyzed.





The glycosylation status of these mutant proteins was directly tested by measuring their sensitivity to glycosidases (Fig. 5). As expected, ACE(T)WT was both N- and O-glycosylated. The same was true for both ACE(T)g2 and ACE(T)g1, although the extent of their N-glycosylation was less than that of ACE(T)WT. The 76-kDa ACE(T)g3 protein, on the other hand, was neither O-glycosylated nor N-glycosylated. It should be noted that for this analysis of ACE(T)g3, we used a relatively larger quantity of an extract of cells that had been pulse-labeled but not chased. Enough of the 76-kDa protein was available for analysis only under these conditions. The lack of glycosylation of ACE(T)g3 was further confirmed by the failure to react with an anti-sugar antibody (data not shown). Thus, ACE(T)g3, although synthesized, remained unglycosylated in the HeLa cells. As a consequence, it was rapidly degraded and not transported to the cell surface. The above conclusions drawn from the metabolic labeling experiments ( Fig. 4and Fig. 5) were confirmed by immunofluorescence studies (Fig. 6). ACE(T)WT was present both on the surface and inside of the transformed cells. In contrast, a small amount of ACE(T)g3 was present inside the cells, but none was displayed on the cell surface. The pattern of intracellular distribution of ACE(T)g3 suggests that the protein is arrested in the endoplasmic reticulum. Taken together, these experiments demonstrate that all glycosylation sites in ACE(T) are not equivalent. Site 3, by itself, is not sufficient for glycosylation of the protein, but sites 1 and 2 are.


Figure 5: N- and O-glycosylation status of mutants with single N-glycosylation sites. HeLa cells were transfected with ACE(T)WT (lanes 1-4), ACE(T)g2 (lanes 5-8), ACE(T)g1 (lanes 9-12), and ACE(T)g3 (lanes 13-16) cDNAs. After pulse-labeling, the label was chased for 0 h (ACE(T)g3-transfected cells) or 15 h (all other cells). Cell lysates were immunoprecipitated, boiled with SDS, and left untreated(-) or treated with (+) deglycosylating enzymes as indicated. For the analysis of ACE(T)g3 (lanes 13-16), five times more extract was used than all other lanes.




Figure 6: Absence of ACE(T)g3 on the cell surface: detection of ACE(T)WT and ACE(T)g3 proteins by indirect immunofluorescence. HeLa cells grown on coverslips were transfected with ACE(T)WT or ACE(T)g3 cDNA. Transfected cells were processed for indirect immunofluorescence as described under ``Experimental Procedures'' using anti-ACE antibody and fluorescence-conjugated rabbit anti-goat IgG, to detect ACE proteins expressed intracellularly (Internal) or on the cell surface (Surface).



Expression of ACE(T) in Yeast

In the next series of experiments, we attempted the expression of ACE(T) in yeast. The methylotropic strain P. pastoris was chosen because of the ease of induced production of a larger quantity of a foreign protein. ACE(T)WT, ACE(T)g13, ACE(T)g2, and ACE(T)g3 were cloned in the appropriate yeast expression vector. After transformation and growth, the introduced genes were induced, and the expression of ACE(T) was monitored. For these experiments, instead of detection by metabolic labeling and immunoprecipitation, ACE(T) proteins secreted in the culture medium were detected by Western blotting. As shown in Fig. 7, ACE(T)WT was efficiently synthesized and secreted by P. pastoris. The secreted protein had a molecular mass of 90 kDa and was enzymatically active (Table 2). Thus, unlike E. coli, P. pastoris was able to synthesize an active form of ACE(T). ACE(T)g13 and ACE(T)g2 were similarly synthesized in yeast (Fig. 7). As in HeLa cells, ACE(T)g2 was slightly bigger than ACE(T)g13. Surprisingly, ACE(T)g3 was also synthesized and secreted by yeast. This protein had the lowest molecular mass (70 kDa) of all the ACE(T) mutants expressed, and it was enzymatically active (Table 2). The glycosylation status of ACE(T)WT and ACE(T)g3 expressed in yeast was examined (Fig. 8). Similar to the proteins produced in HeLa cells, these proteins were N-glycosylated, although to different extents, but insensitive to O-glycosidase treatment. It appears that the third glycosylation site, present at residue 145 of ACE(T)g3, was recognized and used in yeast. As a result, the ACE(T)g3 protein synthesized in yeast was stable and secreted. These results demonstrated a remarkable difference between yeasts and mammalian cells with respect to the use of specific potential glycosylation sites of a protein.


Figure 7: Expression of ACE(T)WT, ACE(T)13, ACE(T)g2, and ACE(T)g3 in methylotropic yeast, P. pastoris. ACE(T)WT and three other mutant proteins were expressed in P. pastoris as described under ``Experimental Procedures.'' Two days after methanol induction, 40 µl of the clarified culture media were analyzed by Western blot analysis using anti-ACE antibody. Lane 1, ACE(T)WT; lane 2, ACE(T)g13; lane 3, ACE(T)g2; and lane 4, ACE(T)g3. Numbers on the left indicate the molecular masses of the expressed proteins in kilodaltons.



Finally, the mode of secretion of ACE(T)WT and ACE(T)g3 from yeast was examined. We have shown previously that secretion of ACE(T) from mammalian cell surface is accomplished by proteolytic cleavage of the ectodomain. As a result, the secreted form of ACE(T) does not contain the membrane-anchoring domain and the intracellular domain present in the cell-bound form of ACE(T). In the experiment shown in Fig. 9, we examined if the ACE(T) secreted by yeast also lacks these domains. For this purpose, we used a COOH-terminal-specific antibody, which reacts with HeLa cell-bound ACE(T), but not with secreted ACE(T)(23) . This antibody also failed to react with ACE(T)WT and ACE(T)g3 secreted by yeast (Fig. 9), thus indicating that ACE(T) is also cleavage-secreted by P. pastoris.


Figure 9: Cleavage secretion of ACE(T) in P. pastoris. Culture media of P. pastoris expressing ACE(T)WT (lanes 1 and 3, 20 µl each) and ACE(T)g3 (lanes 2 and 4, 60 µl each) were analyzed by Western blot analysis using anti-ACE (lanes 1 and 2) or anti COOH-terminal peptide antibody (lanes 3 and 4).




DISCUSSION

We have established transfected mammalian cell lines that are high producers of enzymatically active ACE(T)(14) . We purified large quantities of ACE(T) from the culture medium of these cells and attempted its crystallization without any success. We reasoned that the failure to crystallize could be due to an inherent heterogeneity of the purified protein which, in turn, is caused by its high level of glycosylation. We, therefore, sought out means to produce unglycosylated and underglycosylated ACE(T). There are reports in the literature that the sugars may not be required for the activity of ACE(P), since purified ACE(P) can be deglycosylated without losing activity(26) . These studies, however, provided no evidence that the deglycosylation was complete, nor do they address the issue of whether newly synthesized unglycosylated ACE can fold into an active conformation. We observed that unglycosylated ACE(T) produced in tunicamycin-treated HeLa cells is inactive and rapidly degraded(20) . In the present study, we, therefore, produced unglycosylated ACE(T) in E. coli. This protein was not degraded in the bacterium, and therefore, it is possible to produce and purify bacterially produced ACE(T) in large quantities. It, however, is devoid of enzyme activity and not suitable for crystallization and structural studies. This result was not entirely unexpected since many mammalian glycoproteins, when produced in bacteria, are biochemically inactive. This lack of activity is ascribed to misfolding of the proteins in bacteria. Consequently, careful denaturation and renaturation of some of these bacterially produced proteins regenerate activity(27) . This, however, could not be accomplished for ACE(T). We, therefore, resorted to alternative methods for producing active ACE(T) with minimum sugar modifications. For this purpose, the glycosylation sites were mutated and the corresponding proteins were produced in HeLa cells and P. pastoris. Our results showed that allowing N-glycosylation only at one out of five sites was sufficient for producing active ACE in both systems. The proteins produced in yeast had, however, consistently lower molecular weights (Table 2), thus suggesting less post-translational modifications of ACE(T) in yeast. In the future, ACE(T)g3 produced in yeast will probably be the best candidate for crystallization.

Our studies with the glycosylation site mutants revealed interesting differences in the nature of contributions of each of the five sites. The sizes of all of the single mutants (Fig. 3A) or all of the double mutants (Fig. 3B) are not the same. This suggests that either the complexity of sugar chains at each of these sites is different or that N-glycosylation at each site has different effects on other modifications of the protein such as O-glycosylation. These mutations also affected the rate of synthesis and the rate of cleavage secretion differently. It would be interesting to investigate in the future why ACE(T)g134 was secreted much more efficiently than ACE(T)g135. Like ACE(T)WT, ACE(T)g2 and ACE(T)g1 produced in HeLa cells were both N- and O-glycosylated (Fig. 5). These proteins probably carry additional modifications whose nature remains to be determined. Note that completely deglycosylated ACE(T)WT, g2 and g1 (lanes 4, 8, and 12, Fig. 5) had a molecular mass much higher than 76 kDa, which was the molecular mass of unglycosylated ACE(T)g3 (lane 13, Fig. 5). All of these proteins, when produced in yeast, had molecular weights lower than the corresponding species produced in HeLa cells (Table 2). This could be due to either a lack of O-glycosylation of ACE(T) produced in yeast, or it could be that the other putative additional modifications, be it phosphorylation, sulfation, or others, are missing in yeast. Completely deglycosylated secreted form of ACE(T)WT produced in yeast had a molecular mass of about 70 kDa (lane 4, Fig. 8), whereas the corresponding molecular mass of secreted, deglycosylated ACE(T)WT produced by HeLa cells is about 84 kDa (data not shown). Since ACE(T) produced in yeast was enzymatically active, the additional modifications are obviously not needed for the formation of active ACE. These modifications of ACE(T) probably occur in the Golgi or plasma membrane of HeLa cells, since ACE(T)g3 arrested in the endoplasmic reticulum seems to be devoid of them.

ACE(T) produced in P. pastoris was cleavage-secreted (Fig. 9), since the secreted ACE(T) protein was devoid of the cytoplasmic tail. The exact cleavage site has not yet been determined and compared with the site used in mammalian cells. The cleavage secretion process in the mammalian cells is regulated by phorbol esters and the responsible plasma membrane-associated proteolytic activity has the characteristics of a specific class of metalloprotease(28) . It remains to be determined if the cleavage secretion process in yeast has similar characteristics. If so, it can serve as a model for the mammalian activity, and the responsible protease may be more easily identifiable in the yeast system.

The observed differential behaviors of ACE(T)g3 in HeLa cells and P. pastoris were unexpected. We are unaware of any other examples of a mammalian glycoprotein that fails to be glycosylated in the endoplasmic reticulum of a mammalian cell, but not in yeast. ACE(T)g3 can, thus, serve as a useful tool for identifying key differences in the glycosylation apparatus of the two cell types. Since yeasts are widely used for studying the process involved in eukaryotic protein trafficking and post-translational modifications, the differences noted here may serve as a warning that all the steps may not be equivalent in yeast and higher eukaryotes.


FOOTNOTES

*
This work was supported in part by the National Institutes of Health Grant HL-48258 and a grant-in-aid from the American Heart Association, Northeast Ohio Affiliate. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
To whom correspondence should be addressed: Dept. of Molecular Cardiology, The Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, OH 44195. Tel.: 216-444-9057; Fax: 216-444-9263.

(^1)
The abbreviations used are: ACE, angiotensin-converting enzyme; ACE(P), pulmonary angiotensin-converting enzyme; ACE(T), testicular angiotensin-converting enzyme; PAGE, polyacrylamide gel electrophoresis; WT, wild type.


ACKNOWLEDGEMENTS

We are grateful to Ganes C. Sen of the Department of Molecular Biology, The Cleveland Clinic Foundation, for providing constructive suggestions. We thank JoAnne Holl for secretarial assistance.


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