©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
DNA Binding and Dimerization of the FeS-containing FNR Protein from Escherichia coli Are Regulated by Oxygen (*)

(Received for publication, September 1, 1995; and in revised form, November 20, 1995)

Beth A. Lazazzera (1) Helmut Beinert (2) Natalia Khoroshilova (3) Mary Claire Kennedy (4) Patricia J. Kiley (3)(§)

From the  (1)Department of Bacteriology, (2)Institute for Enzyme Research and Department of Biochemistry, and (3)Department of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin 53706 and (4)Department of Biochemistry and Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, Wisconsin 53226

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

The transcription factor FNR from Escherichia coli regulates transcription of genes in response to oxygen deprivation. To determine how the activity of FNR is regulated by oxygen, a form of FNR had to be isolated that had properties similar to those observed in vivo. This was accomplished by purification of an FNR fraction which exhibited enhanced DNA binding in the absence of oxygen. Iron and sulfide analyses of this FNR fraction indicated the presence of an Fe-S cluster. To determine the type of Fe-S cluster present, an oxygen-stable mutant protein LH28-DA154 was also analyzed since FNR LH28-DA154 purified anoxically contained almost 3-fold more iron and sulfide than the wild-type protein. Based on the sulfide analysis, the stoichiometry (3.3 mol of S/FNR monomer) was consistent with either one [4Fe-4S] or two [2Fe-2S] clusters per mutant FNR monomer. However, since FNR has only four Cys residues as potential cluster ligands and an EPR signal typical of a 3Fe-4S cluster was detected on oxidation, we conclude that there is one [4Fe-4S] cluster present per monomer of FNR LH28-DA154. We assume that the wild type also contains one [4Fe-4S] cluster per monomer and that the lower amounts of iron and sulfide observed per monomer were due to partial occupancy. Consistent with this, the Fe-S cluster in the wild-type protein was found to be extremely oxygen-labile. In addition, molecular-sieve chromatographic analysis showed that the majority of the anoxically purified protein was a dimer as compared to aerobically purified FNR which is a monomer. The loss of the Fe-S cluster by exposure to oxygen was associated with a conversion to the monomeric form and decreased DNA binding. Taken together, these observations suggest that oxygen regulates the activity of wild-type FNR through the lability of the Fe-S cluster to oxygen.


INTRODUCTION

The ability to sense and adapt to changes in oxygen concentration is critical to the survival of many organisms. In Escherichia coli, the transcription factor FNR plays a central role in allowing the bacterium to adapt to changes in oxygen availability in its environment(1) . When oxygen is limiting, FNR activates synthesis of many enzymes required to generate energy by anaerobic respiration and also represses synthesis of some enzymes involved in aerobic respiration. Since the levels of FNR do not significantly differ between aerobically and anaerobically grown cells, oxygen deprivation must modulate FNR-dependent transcription by regulating the activity of FNR(2) . Determining how oxygen regulates FNR activity should provide fundamental information regarding the strategies available to cells to sense changes in oxygen levels.

The isolation and analysis of FNR mutant proteins that are active in the presence of oxygen (FNR*) has provided important clues as to how wild-type FNR activity might be regulated by oxygen(3, 4, 5) . For example, the in vitro analysis of one FNR* mutant protein, FNR-DA154 which has an Asp to Ala substitution at position 154, led to the initial proposal that oxygen regulates the oligomeric state of FNR. When purified in the presence of oxygen, FNR-DA154 protein showed increased dimerization (3) and DNA binding (3, 5) relative to the monomeric wild-type protein(3, 6) . This suggested that wild-type FNR becomes active under anaerobic conditions because of an increase in the formation of FNR dimers that presumably have maximal DNA binding affinity. In addition, the analysis of another FNR* mutant protein, FNR-LH28-DA154 which contains a Leu to His substitution at position 28 in addition to the DA154 substitution, identified a cofactor, an Fe-S cluster. This Fe-S cluster was associated with aerobically purified FNR-LH28-DA154 protein, and the association of this cluster enhanced DNA binding by the mutant protein(4) .

Taken together, the results from the analysis of the FNR* mutant proteins led us to consider that, under anaerobic conditions, wild-type FNR contains an Fe-S cluster that increases dimerization and DNA binding. Consistent with this proposal, wild-type FNR requires Fe for activity in vivo(7, 8) , and FNR possesses four cysteines that are essential for FNR function and that could serve as potential ligands for an Fe cofactor(9, 10, 11) . However, previously purified wild-type FNR did not contain an Fe-S cluster but contained Fe at a stoichiometry of up to 1 mol of Fe per monomer of FNR(6) . This Fe appeared to be loosely associated since its presence depended on providing Fe in the purification buffer. Although Fe bound to FNR in this way had little effect on DNA binding and FNR was still monomeric(6) , some functional significance was attributed to the presence of this loosely bound Fe, because this form of FNR was reported to increase open complex formation at an FNR-dependent promoter(12, 13) . To reconcile the apparent differences in the type of Fe cofactor associated with wild-type FNR and the FNR* mutant protein, we have considered that wild-type FNR may contain an oxygen-labile Fe-S cluster which is required for increased DNA binding and dimerization in the absence of oxygen, and that the Fe found associated with purified wild-type FNR may be a remnant of a labile cluster.

A potential reason for the different properties of purified FNR* proteins and wild-type protein is that, until recently, a reliable functional assay was lacking to monitor purification of FNR. Thus, previously isolated FNR may have lost a component(s) during purification, such as an Fe-S cluster, necessary for maximum FNR activity. Therefore, we developed an assay to monitor DNA binding by FNR in cell extracts in the presence and absence of oxygen, because analysis of the FNR* mutants suggested that DNA binding by wild-type FNR should be regulated by oxygen. Using this assay, we purified and characterized a form of FNR which exhibited increased DNA binding under anoxic conditions. This preparation of FNR was also found to have an increased ability to dimerize and contained an Fe-S cluster.


MATERIALS AND METHODS

Anoxic Sample Handling

To minimize any exposure to oxygen, samples were manipulated either under a stream of copper-scrubbed argon gas or in an anaerobic chamber (Coy) which had an atmosphere of approximately 5% CO(2), 5% H(2), and 90% N(2). Buffers were sparged with argon gas and then allowed to stand in an anaerobic chamber for at least 16 h. All sample-handling devices and test tubes were rinsed with anoxic buffers containing the reductant sodium dithionite. When it was necessary to work with protein samples lacking dithionite, this reductant was removed from all sample-handling materials by rinsing with anoxic buffer lacking dithionite.

DNA Binding Assay

The extent of DNA binding by FNR was assessed by a gel-retardation assay essentially as described by Lazazzera et al.(3) . Briefly, protein was mixed in a 10-µl volume to achieve a final concentration of 5 nM of a 48-base pair DNA fragment containing the FNR-consensus site, 0.1 M potassium glutamate (pH 7.5), 10 mM potassium phosphate (pH 7.5), 1 mM potassium EDTA (pH 7.5), 3 mM Tris-Cl (pH 7.9), 50 µg/ml bovine serum albumin (Pierce), 30 µg/ml calf thymus DNA, and 5% glycerol (Fisher, enzyme grade). When the assays were performed anoxically, sodium dithionite (Fluka, MicroSelect grade) (570 mM in 0.1 M Tris-Cl (pH 7.9)) was added to a final concentration of 1.7 mM. Following incubation at 37 °C for 10 min, the reaction mixture was loaded onto a 10% polyacrylamide gel (38:2 acrylamide/bis-acrylamide ratio) in Tris borate-EDTA buffer(14) , which had been electrophoresed for 30 min prior to loading, and was then run at 150 V for an additional hour. The amount of DNA bound by FNR was quantitated using a PhosphorImager from Molecular Dynamics.

Purification of FNR Protein

Cell extracts were prepared from an E. coli B derivative, PK22 (Deltacrp-bs990 and Deltafnr), carrying pPK823 (3) or pPK2012(4) . These strains have either wild-type fnr or fnr*LH28-DA154, respectively, under the control of a T7 promoter. Cells containing wild-type fnr were grown at 37 °C in 4 liters of Luria-Bertani medium plus M9 salts (15) , 0.2% glucose, and 50 µg/ml ampicillin in an anaerobic chamber. Cells containing fnr*LH28-DA154 were grown as described previously(4) . When the cells reached an OD of 0.35-0.60, freshly prepared isopropyl-thio-beta-D-galactoside was added to a final concentration of 400 µM, and the cells were incubated for 1 h to induce FNR synthesis. The cells were then chilled on ice, and all subsequent steps in the preparation of extracts were carried out at 4 °C under anoxic conditions. Cells were harvested by centrifugation, resuspended to 0.005 the original volume of the cells in buffer (50 mM potassium phosphate (pH 6.8), 0.1 M KCl, 10% glycerol (Fisher, enzyme grade), 0.1 mM phenylmethylsulfonyl fluoride, and 1.7 mM sodium dithionite) and passed once through a French press at 20,000 p.s.i. The extracts were centrifuged in a SS-34 rotor at 7000 rpm for 30 min to remove cell debris and subsequently centrifuged at 45,000 rpm in a Beckman 70.1 Ti rotor for 1 h to remove the membrane fraction. The resulting supernatant was the cell extract assayed for DNA binding and the starting material for FNR purification.

FNR protein was partially purified from cell extracts by fractionation over a Bio-Rex 70 column essentially as described previously (3) but employing the following modifications. All steps in the purification were done under anoxic conditions at room temperature. The buffers contained 1.7 mM sodium dithionite and lacked EDTA and dithiothreitol. One-ml fractions were assayed for DNA binding, and the fractions containing the peak of DNA binding by FNR were pooled.

Determination of Protein Concentration and Purity

Protein concentrations were determined using the Bradford (16) assay and the Coomassie Plus protein assay reagent (Pierce) and bovine serum albumin as a standard. In addition, the purified FNR preparations were analyzed for protein concentration by amino acid analysis (MicroChemical Facility, University of Minnesota). The protein concentration as determined by this method was 1.33-fold lower than that determined by Bradford assay, and all protein concentrations for purified FNR determined by Bradford assay were corrected by this amount. In conjunction with Fe and S determinations, protein was assayed by a biuret procedure, preceded by a trichloroacetic acid precipitation to eliminate interfering substances. This method was also standardized against the amino acid analysis.

The purity of the FNR protein preparations was estimated from Coomassie-stained SDS-polyacrylamide gels. The percentage of the cell extract protein which was FNR protein was determined by analyzing Western blots (17) of SDS-polyacrylamide gels run with multiple concentrations of cell extract protein and purified FNR protein. The gels or blots were scanned using a Color One Scanner (Apple Computer Inc.) and processed using Scan Analysis software (BioSoft) (data not shown).

Gel-exclusion Chromatography

To determine the apparent molecular weight of FNR protein preparations, 200-µl protein samples at FNR concentrations ranging from 12 to 17 µM were passed over a Superose 12 HR (Pharmacia Biotech Inc.) column (10 times 300 mm) at a flow rate of 0.5 ml/min. The column was preequilibrated with anoxic buffer (10 mM KPO(4) (pH 6.8), 10% glycerol, and 0.4 M KCl) which lacked dithionite. The eluent from the Superose column was monitored for protein by the absorbance at 280 nm. Bovine serum albumin, carbonic anhydrase, and cytochrome c protein standards (Sigma) were used to calibrate the column.

Optical Spectroscopy

To remove dithionite from the protein for recording of spectra, the protein solution was diluted in buffer [10 mM KPO(4) (pH 6.8), 10% glycerol] to achieve a final concentration of 0.1 M KCl. This diluted protein was loaded on a one ml Bio-Rex 70 column which had been equilibrated with the same buffer. The protein was eluted from the column with one ml of buffer containing 0.8 M KCl. Spectra of this protein were recorded using a Lambda 2 spectrophotometer (Perkin-Elmer); anaerobiosis was maintained by capping cuvettes which were filled in an anaerobic chamber.

Iron and Sulfide Analysis

Iron and sulfide were determined, as described in Kennedy et al.(18) and Beinert (19) respectively, on three independent samples concentrated as described above for optical spectroscopy and analyzed in duplicate. To verify the formation of methylene blue from S and p-phenylenediamine, the ratio of absorbancies at 670, 710, and 750 nm was regularly measured when the color of methylene blue was not obvious to the eye (OD < 0.160 at a 1-cm path length)(19) .

Low Temperature EPR

EPR spectra were recorded at 9.11 GHz and 2 mW at 13 K with a modulation amplitude of 0.5 mT at 100 KHz.


RESULTS

DNA Binding of FNR Is Enhanced under Anoxic Conditions

To develop an in vitro assay for detecting oxygen regulation of FNR activity, we chose to monitor changes in DNA binding by FNR, since previous analysis of FNR* mutant proteins (3, 4, 5) suggested that DNA binding of wild-type FNR should be increased under anaerobic conditions. FNR was assayed for DNA binding by a gel-retardation assay. Using an extract prepared from anaerobically grown cells in which fnr is under T7 promoter control, a protein-DNA complex, which was dependent on FNR being present in the extracts (data not shown), was observed (Fig. 1A, lane 6). The size of this complex was identical to that observed with purified FNR from aerobic cells (data not shown), indicating that no additional proteins were present in the complex, and the electrophoretic mobility of the complex was the same whether the complex was formed in the presence or absence of oxygen (Fig. 1A, lanes 6 and 12). In addition, DNA binding by FNR appeared to be specific for the FNR-target site because the assay mixture also contained excess nonspecific, calf thymus DNA.


Figure 1: DNA binding by FNR present in anoxically prepared cell extracts. A, gel-retardation assays were performed as described under ``Materials and Methods'' under anoxic conditions (lanes 1-6) and under aerobic conditions (lanes 7-12). The concentrations of cell extract protein assayed in lanes 1-6 and 7-12 are 0, 14, 28, 56, 110, and 220 ng/µl respectively. The migration of the 2.7-kilobase pair plasmid DNA (Vector DNA), the 48-base pair DNA fragment containing the consensus FNR site (FNR-Target DNA), and the FNR-DNA complex (Complex) are indicated. B, quantitation of the percentage of the 48-base pair DNA fragment bound by FNR assayed under anoxic conditions () or aerobic conditions () as a function of the concentration of protein in anaerobic cell extracts.



The amount of DNA binding by FNR that was present in the cell extracts, was also regulated by oxygen (Fig. 1). Under anoxic conditions, FNR bound approximately 90% of the DNA at the highest concentrations of protein assayed (Fig. 1A, lanes 2-6, and Fig. 1B). However, assaying these same extracts in the presence of oxygen decreased the amount of DNA bound to 20% even at the highest concentrations of protein assayed (Fig. 1A, lanes 8-12, and Fig. 1B), which is similar to the amount of DNA bound by FNR purified from aerobic cells(5) .

FNR Purified under Anoxic Conditions Exhibits Oxygen-regulated DNA Binding

To determine what property increased DNA binding by FNR in the absence of oxygen, we partially purified a form of FNR that demonstrated oxygen-regulated DNA binding. Extracts from anaerobically grown cells were fractionated by cation-exchange chromatography under anoxic conditions. A peak of DNA binding by FNR which was sensitive to oxygen was eluted at approximately 0.36 M KCl (Fig. 2). In contrast, the majority of FNR protein was eluted at approximately 0.27 M KCl (Fig. 2). This is also the concentration of KCl at which the majority of FNR is eluted under aerobic conditions (data not shown). The column fractions which exhibited oxygen-regulated DNA binding were pooled and were shown to contain approximately 90% FNR as judged by SDS-PAGE analysis (Fig. 3, lane 2). This FNR preparation exhibited properties similar to FNR present in cell extracts in that 90% of the DNA was bound at the highest concentrations of protein assayed under anoxic conditions, and only 20% of the DNA was bound under aerobic conditions (Fig. 4).


Figure 2: Fractionation of anoxically prepared cell extracts by cation-exchange chromatography (Bio-Rex 70). FNR fractions that contained oxygen-regulated DNA binding were identified by assaying 1 µl of every third fraction and plotting the percentage of the FNR target DNA bound by these fractions either under anoxic conditions plus dithionite (box) or aerobic conditions (). The molar concentration of KCl used to elute FNR(- - - - -) is also plotted. Shown above the graph is the section of a SDS-polyacrylamide gel which contains 10 µl of every third fraction to show the amount of FNR protein in each fraction.




Figure 3: SDS-polyacrylamide gel electrophoresis analysis of FNR that was anoxically purified by cation-exchange chromatography. Lane 1 contains the cell extract (10 µg) loaded on to the Bio-Rex 70 column and lane 2 shows 10 µg of the pool of Bio-Rex 70 fractions containing the peak of oxygen-regulated DNA binding by FNR.




Figure 4: DNA binding by anoxically purified FNR. Quantitation of the percentage of the FNR DNA-target site bound by FNR under anoxic conditions in the presence of dithionite () or aerobic conditions () as a function of protein concentration.



Despite the recovery of a form of FNR which behaved similar to that found in the cell extracts, only a fraction of the oxygen-regulated DNA binding activity that was originally present in cell extracts was recovered in this purification step (Table 1). This was determined by two criteria. First, the 5-fold increase in the purity of the FNR protein following cation-exchange chromatography (Fig. 3) does not correspond to the 1.5-fold decrease in the amount of FNR protein required for half-maximal DNA binding. Second, only approximately 30% of the oxygen regulated DNA binding activity present in cell extracts was recovered in the purified fraction. This loss of FNR which possessed oxygen-regulated DNA binding activity suggests that this activity is labile. In addition, it was difficult to further purify this form of FNR, since such attempts resulted in a substantial loss of oxygen-regulated DNA binding.



The Apparent Molecular Weight of FNR Changes on Exposure to Oxygen

Previous in vivo analysis of FNR dominant-negative mutants indicated that FNR was converted from a monomer to an oligomer in response to oxygen deprivation(3) . However, all previous preparations of wild-type FNR yielded only monomeric protein(3, 6) . Therefore, to test in vitro if the oligomeric state of FNR was increased in the absence of oxygen as was indicated by our in vivo experiments, we examined the size of the anoxically purified FNR protein by gel-exclusion chromatography (Fig. 5). When the cation-exchange purified fraction of FNR, which showed oxygen-regulated DNA binding, was analyzed under anoxic conditions, the majority of the protein was eluted in a broad peak with a molecular weight which varied from 56,000 to 45,000, depending on the protein preparation analyzed. A dimer of FNR would have a molecular weight of 60,000, and therefore, the molecular weights observed most likely represent dimers of FNR which are dissociating during the analysis. When this FNR preparation was briefly exposed to air (less than 1 min) prior to loading the protein on the gel-exclusion column, the distribution of monomers and dimers was reversed. Only a portion of the FNR protein remained dimeric, and the majority was eluted with a molecular weight of an FNR monomer, 30,000. It seems likely that further dissociation of FNR would occur during longer exposures of FNR to oxygen, but precipitation of the protein under these conditions prevented such an analysis. These data are consistent with our previous in vivo analysis of FNR dominant-negative mutants that suggested that the form of wild-type FNR active in DNA binding is a dimer and that the oligomeric state of FNR is regulated by oxygen. Thus, this purified form of FNR has the same characteristics that wild-type FNR was observed to have in vivo.


Figure 5: Gel-filtration analysis of anoxically purified FNR. The absorption at 280 nm of the eluent from the Sepharose 12 HR column has been plotted versus the elution volume in milliliters. Shown are the elution profiles of the pooled Bio-Rex 70 fractions run on the column in the absence (A) and presence (B) of oxygen as described under ``Materials and Methods.'' The presence of FNR in the two fractions indicated by arrows was verified by SDS-PAGE. C, the molecular weight of the protein in these two fractions was determined by comparison to the following standards: BSA, carbonic anhydrase, and cytochrome c. The peaks corresponding to an apparent dimer and monomer of FNR are indicated by the arrows. The large slowly eluting peak at 18 ml is dithionite.



Spectral Properties of Anoxically Purified FNR

The anoxically purified FNR protein possessed a yellow color which suggested that a cofactor(s) was associated with this protein that could be responsible for the increased dimerization and DNA binding properties. To determine the nature of the compound(s) that might be imparting this color to FNR, ultraviolet/visible spectra of the protein were recorded under anoxic conditions (Fig. 6). In addition to the protein absorption maximum at 280 nm, a shoulder at 320 nm and a broad peak with an absorption maximum around 420 nm were observed. This is similar to that observed for [4Fe-4S]-containing proteins (20) and the aerobically purified FNR* mutant protein, FNR-LH28-DA154, which contains an Fe-S cluster(4) .


Figure 6: Ultraviolet/visible absorption spectrum of anoxically purified FNR protein analyzed at 2.0 mg/ml.



An Iron-Sulfur Cluster Is Associated with FNR

To determine whether the spectral properties imparted on the FNR protein were due to an Fe-S cluster, iron and sulfide analyses were performed. These showed the presence of iron which ranged from 0.44 to 1.9 mol of iron/mol of FNR monomer and sulfide which ranged from 0.36 to 1.2 mol of inorganic sulfide/mol of FNR monomer, indicating that an Fe-S cluster was associated with FNR. To determine the type of Fe-S cluster that was associated with FNR, low temperature EPR spectroscopy was performed. In some preparations, only a small signal typical of [3Fe-4S] clusters (g = 2.01) was observed, which accounted for only a small fraction of the Fe-S cluster concentration expected from sulfide determination (data not shown). This signal increased severalfold upon addition of ferricyanide (data not shown). This suggests that an EPR silent [4Fe-4S] may have been associated with the FNR protein as [3Fe-4S] clusters are known to arise only from [4Fe-4S] clusters but not from [2Fe-2S] centers(21) . We have not yet been able to detect a signal for a [4Fe-4S] cluster (g 1.9) on reduction at pH 8.5 with dithionite in the presence of methyl viologen. Explanations for this could be that the 1+ form has a very low redox potential or has a spin S > 1/2. The finding of [3Fe-4S] clusters, together with results of the chemical analyses of wild-type and mutant proteins (see below) and the knowledge that in vivo FNR requires four Cys per monomer, can only be reconciled with our current understanding of Fe-S clusters if there is one [4Fe-4S] cluster present per monomer. Based on this interpretation, the maximal amount of [4Fe-4S] cluster found in wild-type FNR was 0.32 mol of cluster/mol of FNR monomer. We think that it is unlikely that the Fe-S cluster belongs to a contaminating protein in the preparations, since no single contaminating protein represented 32% of the total protein, and since with the same protein fraction from a cell extract lacking FNR under anoxic conditions, the typical absorption in the visible region was not observed (data not shown).

The Fe-S Cluster in Wild-type FNR Is Oxygen-labile

The finding of an Fe-S cluster associated with anoxically purified FNR suggests that this cluster is required for the increased DNA binding and dimerization of this protein. Because these activities are decreased in the presence of oxygen, we examined the changes in the Fe-S cluster after exposure to oxygen by ultraviolet/visible spectroscopy (Fig. 7). There was an initial (within 1 min) drop in the absorption in the visible region (Fig. 7, dashed line) indicating loss of the Fe-S cluster, followed (starting at 10 min) by a general increase in absorbance at all wavelengths due to protein precipitation (Fig. 7, stippled line), suggesting the cluster associated with FNR is being degraded in the presence of oxygen. Consistent with this, FNR purified under aerobic conditions showed no absorption maxima other than that at 280 nm and did not contain inorganic sulfide (6) (data not shown). These data suggest that the oxygen lability of the Fe-S cluster associated with anoxically purified FNR is the cause for the decrease in DNA binding and dimerization in the presence of oxygen.


Figure 7: Effect of oxygen on ultraviolet/visible spectrum of FNR. Shown are the spectra of FNR (1.2 mg/ml) in the absence of oxygen (solid line) and 1 min (stippled line) and 20 min (dashed line) after exposure to oxygen.



Two [4Fe-4S] Clusters Are Associated with a Dimer of Anoxically Purified FNR* Mutant Protein, FNR-LH28-DA154

Unlike the wild-type protein, when the FNR* mutant protein, FNR-LH28-DA154, was purified in the presence of oxygen(4) , a small fraction of this protein contained an Fe-S cluster, suggesting that the amino acid substitutions in this protein increase the stability of this cluster against oxygen. It seemed possible that the Fe-S cluster associated with this FNR* mutant protein would be further stabilized by the absence of oxygen. To determine if this was true, we purified FNR-LH28-DA154 protein under anoxic conditions. FNR-LH28-DA154 protein was resolved on the cation-exchange column under anoxic conditions into the same fractions as seen under aerobic conditions (data not shown). The anoxically purified protein differed from that purified aerobically in that the protein was more visibly colored (data not shown), suggesting that a greater percentage of the anoxically purified FNR-LH28-DA154 protein contained an Fe-S cluster.

Low temperature EPR spectroscopy was performed on the anoxically purified FNR-LH28-DA154 protein (data not shown). Under anoxic conditions, the protein was EPR silent as would be expected for a [4Fe-4S] cluster. When the protein was oxidized by the addition of potassium ferricyanide, a signal typical of a [3Fe-4S] cluster was detected. This strongly indicates that the type of cluster which was present under anoxic conditions and was EPR silent must have been a [4Fe-4S] cluster (see above). Thus, it appears that the amino acid substitution in the FNR* mutant protein stabilizes the association of the same [4Fe-4S] cluster that is found with wild-type FNR. To determine the stoichiometry of the [4Fe-4S] cluster relative to the FNR-LH28-DA154 protein, iron and sulfide analyses were performed. Iron and sulfide were found associated at maximally 4.0 mol of iron and 3.3 mol of inorganic sulfide/mol of FNR-LH28-DA154 monomer. Based on the concentration of inorganic sulfide, the stoichiometry was calculated to be 1.65 mol of [4Fe-4S] cluster/mol of FNR dimer which indicates that there are two [4Fe-4S] clusters per dimer of FNR-LH28-DA154 protein.


DISCUSSION

The aim of this study has been to gain additional insight into the mechanism of the regulation of FNR activity by oxygen. The development of an in vitro functional assay, which mimics how regulation of FNR occurs in vivo, provides an important tool to dissect what factors are involved in mediating oxygen regulation. The purification of a form of FNR which has oxygen-regulated activity has shown that an Fe-S cluster is associated with wild-type FNR and that this Fe-S cluster appears to be required for the increased DNA binding and dimerization of this protein in the absence of oxygen. This study also extends the previous analysis of an FNR* mutant protein, FNR-LH28-DA154, which was more suitable for determining the stoichiometry of the Fe-S cluster relative to the FNR protein.

FNR Contains Two [4Fe-4S] Clusters per Dimer

As cysteines are often the ligands for Fe-S clusters, it seems likely that the four cysteines shown to be essential for FNR function (9, 10, 11) are the ligands for the Fe-S cluster in this protein. The location of these cysteines is shown in a hypothetical model of the FNR protein (Fig. 8), which is based on the structure of the CAP protein (22) , since these proteins show similarity at the amino acid sequence level(23) . Three of the essential cysteine residues (Cys-20, Cys-23, and Cys-29) are located in the N-terminal domain of FNR, and a fourth cysteine (Cys-122) is located in a region of FNR analogous to the CAP beta-roll structure. The spacing of these cysteines in FNR, with three closely spaced and one distant, is typical of other Fe-S-containing proteins(24) . The finding of up to 3.3 mol of labile sulfide/mol of FNR LH28-DA154 monomer, the knowledge that there are only five total Cys residues available per FNR monomer, together with the observation of [3Fe-4S] clusters on oxidation, can only be interpreted as one [4Fe-4S] cluster per monomer according to our present knowledge of Fe-S clusters. [2Fe-2S] clusters of the ``Rieske'' type are thought to have two Cys and two His ligands per cluster. However, three-dimensional structures are not available yet, and mutation studies have shown that, nevertheless, four Cys residues per cluster have to be present for formation of one of these clusters. They have been found only in electron transfer chains or dioxygenases.


Figure 8: Model for the association of the Fe-S cluster with FNR and regulation of FNR activity by oxygen. A, a hypothetical model of an FNR monomer. This was constructed by attaching the N-terminal extension of FNR (depicted by a thin line) to the existing ribbon diagram of the CAP structure(22) ; the letters and numbers are the CAP nomenclature for alpha-helices and beta-sheets, respectively. Indicated in gray is the dimerization, C-helix. The N terminus of FNR in the presence of the Fe-S cluster (solid line) and in the absence of the Fe-S cluster (dashed line) is indicated, as well as the structure of a [4Fe-4S] cluster. B, a model for regulation of FNR activity by the stability of a [4Fe-4S] cluster to oxygen. Inactive refers to the form of FNR that is predominant under aerobic conditions and shows decreased DNA binding activity. Active refers to the form that shows enhanced DNA binding activity and is present predominantly under anaerobic conditions.



If the structure of FNR is similar to that of CAP, then dimerization should occur along the C-helix (Fig. 8). Therefore, it seems most likely that one monomer would provide all the ligands to the cluster since the location of the cysteines would be too far apart to allow the Fe-S clusters to bridge the subunits. To confirm the location of the Fe-S cluster in the FNR protein requires a three-dimensional structure of FNR.

Although both the FNR-LH28-DA154 and wild-type FNR proteins appear to contain a [4Fe-4S] cluster, the percentage of protein containing a cluster was considerably lower for the wild-type protein (32% based on the S) as compared to the FNR-LH28-DA154 protein (82% based on the S). One possible explanation for this result is that the amino acid substitutions in the FNR-LH28-DA154 protein stabilize the cluster against degradation in the presence of oxygen. This is supported by the observation that an Fe-S cluster is associated with the aerobically purified FNR-LH28-DA154 mutant protein but not with wild-type FNR(3, 6) . However, the lability of the Fe-S cluster to oxygen does not seem to fully explain why in wild-type FNR not all the protein contains an Fe-S cluster. Even in anaerobic cell extracts, where enzymes and substrates should be present to assemble an Fe-S cluster, a large percentage of the FNR protein appears to be inactive for DNA binding, suggesting that not all the FNR protein originally present in the cell extracts contains an Fe-S cluster. It may be that naturally only a fraction of the FNR protein contains an Fe-S cluster. Such a situation is seen with the iron-regulatory protein(26) , which occurs in tissues in both the iron-regulatory protein (apo) or aconitase (containing Fe-S) forms. It is also possible that, because FNR, under the control of the T7 promoter, is expressed to high levels in these cells, a factor may be limiting which is required for association of the Fe-S cluster. It may be that the amino acid substitutions in FNR-LH28-DA154 bypass the requirement for this factor. Further experiments will be necessary to determine why the wild-type protein is not fully occupied by the Fe-S cluster.

The fact that the Fe-S-containing form of FNR was not the major FNR fraction observed by cation-exchange chromatography may explain why this form of FNR has not been previously isolated and underscores the importance of having a functional assay for FNR activity. Previously, FNR was purified based on immunoblotting of SDS-polyacrylamide gels for FNR protein(2, 6, 27) , and therefore, it is not surprising that FNR purified based on this criterion did not contain detectable amounts of Fe-S cluster. In addition, it is likely that the lability of the Fe-S cluster to oxygen was also partly responsible for the previous failure to isolate a form of FNR containing the cluster(6) . The one mol of Fe/mol of FNR found in previous studies (6) may be a remnant of an oxygen-labile Fe-S cluster. The inclusion of a strong reductant, dithionite, in the purification buffers may have improved our ability to stabilize the Fe-S cluster.

The Instability of the Fe-S Cluster to Oxygen Regulates the Activity of FNR

The Fe-S cluster of FNR appears to be oxygen labile since absorption in the visible region is lost after exposure of the Fe-S containing FNR to oxygen. Thus, a simple model to explain how the Fe-S cluster can be mediating oxygen regulation of FNR activity in vivo is that degradation of the Fe-S cluster by oxygen regulates the activity of FNR (see Fig. 8). The oxygen lability of the Fe-S cluster strongly suggests that the cluster would only be associated with FNR under anaerobic conditions. The apparent requirement of the Fe-S cluster for increased DNA binding and dimerization of FNR, suggests that degradation of the Fe-S cluster upon exposure to oxygen is the cause for the loss of these properties in the presence of oxygen. The Fe-S-containing form of FNR appears to be also capable of transcription activation, although the presence of RNases in the anoxically purified protein has, so far, precluded a quantitative analysis. (^1)Therefore, the Fe-S cluster of FNR appears to function as a direct oxygen sensor.

The Fe-S clusters of hydrolyase proteins and glutamine phosphoribosylpyrophosphate amidotransferase have been shown to be degraded by oxygen both in vitro and in vivo(29, 30, 31, 32) . A mechanism for this degradation of the Fe-S clusters in the hydrolyase proteins involves the attack by superoxide(29) . Consistent with this, the clusters of some of these proteins have been shown to be protected from degradation by the presence of superoxide dismutase both in vitro and in vivo(29, 32, 33) . It must be pointed out, however, that the Fe-S clusters in hydrolyases have only three Cys ligands and, therefore, one accessible cluster iron to which substrate can be bound. Although it is not certain, it is nevertheless likely that the Fe-S cluster in FNR has four Cys ligands so that a different mechanism of degradation may have to be invoked. Whether superoxide is involved in the degradation of the Fe-S cluster of FNR is not known, and it will be interesting to determine if superoxide dismutase protects the Fe-S cluster of FNR from degradation by oxygen. If FNR is inactivated by superoxide, then the Fe-S cluster of FNR must be exquisitely sensitive to superoxide because FNR activity is lost in vivo under normal atmospheric conditions, whereas the hydrolyase proteins are inactivated in vivo only when the concentration of oxygen is increased.

How Does Association of the Fe-S Cluster Increase FNR Activity?

Since the Fe-S cluster associated with FNR is unstable in the presence of oxygen, we favor the major role of the cluster to be in stabilizing an active conformation of FNR under anaerobic conditions. This conformation could be achieved by a simple reorganization of the N-terminal domain of FNR in the presence of the Fe-S cluster. Non-redox roles for Fe-S clusters have been observed with a number of enzymes, and for at least one protein, glutamine phosphoribosylpyrophosphate amidotransferase, the role of the Fe-S cluster appears to be solely to stabilize a conformation resistant to degradation in vivo(34) . As Fe-S clusters can occur in different oxidation states(24) , it is still possible that oxidation or reduction of the Fe-S cluster could further modulate the activity of FNR.

When the observed in vitro properties of the wild-type and FNR* mutant proteins are considered, the association of an Fe-S cluster appears to stabilize a conformation that increases the dimerization constant. This increase in the number of FNR dimers, which presumably have maximal DNA binding affinity, would result in an increase in transcriptional regulation under anaerobic conditions in vivo. Analysis of FNR* mutants has indicated that amino acid 154 is a critical determinant in dimer formation(3, 25) . This amino acid is located at the putative dimerization interface (as shown in Fig. 8), and in wild-type FNR, this amino acid is a negatively charged aspartic acid. Changing this position to a neutral amino acid, alanine, also results in an increase in dimerization which suggests that under aerobic conditions, the aspartic acid is inhibitory to dimer formation. Therefore, a possible mechanism for the Fe-S cluster increasing the ability of FNR to dimerize is that a long range conformational change is induced by the presence of the Fe-S clusters that either moves the aspartic acid side chain away from the dimer interface or brings in close contact a positively charged group to neutralize Asp-154.

In summary, we have provided evidence that anoxically purified FNR contains a [4Fe-4S] cluster, with a stoichiometry of apparently two clusters per FNR dimer. This Fe-S-containing FNR is capable of oxygen-regulated DNA binding and dimerization. On this basis, we propose that the Fe-S cluster is required for FNR activity and the regulation of this activity by oxygen. The data presented in this study provide important information about how FNR may be functioning as an oxygen sensor. Recently, Fe centers have been found in other regulatory proteins. As far as involvement of Fe-S clusters in regulation is concerned, the iron-regulatory protein is an excellent example as to how Fe-S cluster formation or degradation can determine its function (26) . In addition, the SoxR transcription factor has been shown to contain a [2Fe-2S] cluster(35, 36) . In this case, the protein is activated by oxidative stress in vivo, but the role of the Fe-S cluster in mediating regulation has yet to be elucidated. A different Fe center, a heme moiety, is utilized by the sensor component of the FixJK two-component system of Rhizobium meliloti to regulate the kinase activity of FixL on FixJ in response to oxygen deprivation(28, 37) . Thus, it appears that FNR, as do other proteins, utilizes the versatile coordination and redox chemistry of iron for regulatory functions.


FOOTNOTES

*
This work was supported in part by United States Public Health Service, National Institutes of Health Grant GM45844 (to P. J. K.) and a predoctoral National Research Award GM08349 (to B. A. L.). EPR spectroscopy was carried out at the facilities of the National Biomedical ESR Center supported by National Institutes of Health Resources Grant RR01008. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
Recipient of a Young Investigator Award from the National Science Foundation and the Shaw Scientist Award from the Milwaukee Foundation. To whom correspondence should be addressed: Dept. of Biomolecular Chemistry, 1300 University Ave., University of Wisconsin, Madison, WI 53706. Tel.: 608-262-6632; Fax 608-262-5253.

(^1)
E. C. Ziegelhoffer and P. J. Kiley, unpublished data.


ACKNOWLEDGEMENTS

We thank Mary J. Homer for the advice on performing experiments under anoxic conditions.


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