©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
A Thermally Induced Reversible Conformational Transition of the Tryptophan Synthase Subunit Probed by the Spectroscopic Properties of Pyridoxal Phosphate and by Enzymatic Activity (*)

(Received for publication, September 7, 1995; and in revised form, January 29, 1996)

S. Ashraf Ahmed Peter McPhie Edith Wilson Miles (§)

From the Laboratory of Biochemical Pharmacology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

A reversible thermally induced conformational transition of the beta(2) subunit of tryptophan synthase from Salmonella typhimurium has been detected by use of the pyridoxal 5`-phosphate coenzyme as a spectroscopic probe. Increasing the temperature converts the major form of pyridoxal 5`-phosphate bound to the beta(2) subunit from a ketoenamine species with (max) at 410 nm to a enolimine species with (max) at 336 nm (T(m) = 43 °C) and results in loss of the circular dichroism signal at 410 nm and of fluorescence emission at 510 nm. The results indicate that increasing the temperature favors a conformer of the enzyme that binds pyridoxal 5`-phosphate in a more nonpolar environment and leads to loss of asymmetric pyridoxal 5`-phosphate binding. The internal aldimine between pyridoxal 5`-phosphate and the -amino group of lysine 87 is not disrupted by increased temperature because sodium borohydride treatment of the enzyme at either 15 or 60 °C results in covalent attachment of [4`-^3H]pyridoxal 5`-phosphate. The thermal transition of the beta(2) subunit below 60 °C produces reversible thermal inactivation (T(i) = 52 °C) and occurs at a much lower temperature than the major reversible unfolding at 80 °C (Remeta, D. P., Miles, E. W., and Ginsburg, A.(1995) Pure Appl. Chem. 67, 1859-1866). Our new results indicate that the 410 nm absorbing species of pyridoxal 5`-phosphate is the catalytically active form of the cofactor in the beta(2) subunit and that the low temperature reversible conformational transition disturbs the active site and causes loss of catalytic activity.


INTRODUCTION

Pyridoxal 5`-phosphate (PLP) (^1)serves as the coenzyme for many enzymes that catalyze a wide variety of reactions involved in the metabolism of amino acids (racemization, transamination, beta-elimination, beta-replacement, decarboxylation, etc.)(1, 2) . In every PLP-dependent enzyme, the PLP coenzyme forms an internal aldimine with the -amino group of a lysine residue (see structures in Fig. 1A under ``Results''). This internal aldimine usually exhibits an absorption maximum at 410-430 nm attributed to the resonance stabilized ketoenamine form II in Fig. 1A(3, 4, 5) . The dipolar structure II is favored by a more polar environment. Most PLP enzymes also have peaks at 330-340 nm, which may represent the enolimine tautomer (I in Fig. 1A), a neutral species that prefers a nonpolar environment(3, 4, 5, 6) . Reactions with substrates or inhibitors or changes in pH often lead to marked alterations in the spectrum of the enzyme-bound PLP(2, 5) . Thus PLP serves as a useful chromophoric reporter group for changes in the PLP binding site in enzymes and for reactions with substrates and inhibitors.


Figure 1: Structures and absorption properties of internal aldimines formed between PLP and the -amino group of an enzyme lysine residue. A, structures of the enolimine form I and of the resonance stabilized ketoenamine form II (see Introduction). B, effect of temperature on the absorption spectra of the beta(2) subunit. Absorption spectra of the beta(2) subunit (1.17 mg/ml in Buffer P) were recorded after equilibration for at least 10 min at each temperature shown (17, 30, 40, 50, 60, and 70 °C) and at additional temperatures not shown (21, 25, 35, 45, 55, and 65 °C) using Method 1.



The beta(2) subunit of tryptophan synthase (EC 4.2.1.20) catalyzes a number of PLP-dependent reactions including a beta-elimination reaction with L-serine () and a beta-replacement reaction with L-serine and indole ().

(For reviews see (7, 8, 9) .) PLP serves as a useful spectrophotometric indicator of alterations in the PLP binding site of the beta(2) subunit. For example, the UV-visible spectra (10, 11) and circular dichroism spectra (12, 13) of bound PLP are altered by interaction of the beta(2) subunit with the alpha subunit of tryptophan synthase to form an alpha(2)beta(2) complex and by certain beta(2) subunit mutations in the alpha(2)beta(2) complex(11) .

The three-dimensional structure of the tryptophan synthase alpha(2)beta(2) complex from Salmonella typhimurium revealed that the chains are arranged in a nearly linear alphabetabetaalpha order(14) . The larger beta chain (43,000 M(r)) in the complex has two domains of about equal size, designated the N- and C-domains, which are folded in similar helix/sheet/helix structures. The active sites of neighboring alpha and beta subunits are connected by a buried, 30 Å long hydrophobic tunnel that passes between the N- and C-domains of each beta chain. This unique tunnel serves as a likely conduit of indole from the alpha site to the beta site. The binding site for the PLP coenzyme is located between the N- and C-domains at one end of the tunnel. The single tryptophan in the beta chain, Trp-177, is buried in the N-domain.

We report here that the spectral properties of PLP bound to the beta(2) subunit of tryptophan synthase from S. typhimurium are altered by temperature. The results provide evidence for a reversible thermal transition in the beta(2) subunit at a much lower temperature (T(m) = 41-47 °C) than the temperature (80 °C) at which the beta(2) subunit undergoes a major unfolding transition (15, 16, 17) . (^2)Irreversible inactivation occurs at the higher temperature (T(i) = 77 °C) in the presence of a higher salt concentration that causes protein aggregation(18, 19) . Other studies show that the low temperature transition results in a small, low temperature endotherm in differential scanning calorimetry, perturbation in the environment of Trp-177 but not of tyrosine residues, and loss in the ellipticity of PLP(15, 16, 17) .^2 Our finding that this conformational transition results in loss of activity shows that the conformational transition has biochemical and functional relevance.


EXPERIMENTAL PROCEDURES

Chemicals and Buffer

Buffer P (50 mM sodium phosphate, pH 7.8) and Buffer B (50 mM sodium N,N-bis(2-hydroxyethyl)glycine containing 1 mM EDTA at pH 7.8) were used as indicated. [4`-^3H]PLP (specific activity, 8 times 10^4 cpm/nmol) was prepared previously(20) . The model Schiff base of PLP with L-serine was prepared by mixing 0.05 mM PLP (Sigma) with 50 mML-serine in Buffer P. 1,4-Dioxane (99%; Janssen Chimica) was shaken with heated alumina to remove peroxide prior to use.

Enzymes

The beta(2) subunit from S. typhimurium was purified to homogeneity as described (21) or by heat precipitation of the alpha subunit from the alpha(2)beta(2) complex(22) . The apo beta(2) subunit prepared as described in (12) (21 nmol in Buffer P) was treated with [4`-^3H]PLP (23 nmol) at room temperature; unbound cofactor was removed by gel filtration in Buffer P. Protein concentrations were determined from the specific absorbance at 278 nm (22) .

Spectroscopic and Analytical Methods

Absorption spectra were made using a Hewlett-Packard 8452 diode array spectrophotometer thermostated by a circulating water bath (Method 1) or using a Hewlett-Packard 8450 diode array spectrophotometer thermostated by a Peltier junction temperature controlled cuvette holder (Method 2). An external water bath was interfaced with the Peltier unit for temperature control below 20 °C in Method 2. Absorption spectra of the beta(2) subunit (1.17 mg/ml in Buffer P) were recorded after equilibration for at least 10 min at each temperature (Method 1) or for 11 min at each temperature between 0-20 °C and for 3 min at each temperature between 22-70 °C (Method 2). Because different preparations of beta(2) subunit had different contents of PLP, the absorption values were normalized to millimolar extinction values from the absorbance of the solution at 25 °C and the known extinction coefficient for the beta(2) subunit saturated with PLP at pH 7.6 and 25 °C (E = 5.4 mM cm)(23) . Fluorescence emission at 510 nm with excitation at 420 nm was determined using a Perkin-Elmer model MPF-44B fluorimeter thermostated by a circulating water bath. Circular dichroism measurements (mean residue ellipticity in degrees cm^2/dmol) of the beta(2) subunit in Buffer P were made in a Jasco J-500C spectropolarimeter, equipped with a DP-500N data processor (Japan Spectroscopic Co., Easton, MD) and thermostated by a circulating water bath. Single wavelength melting curves were determined at 360 and 410 nm. Spectrophotometric assays at single wavelengths were made in a Cary 118 spectrophotometer. Aliquots of aqueous solutions containing [4`-^3H]PLP or its reaction products were counted in 1 ml of OptiPhase ``HiSafe II'' (LKB) using a Beckman LS3800 scintillation counter.

Enzyme Assays

One unit of activity in any reaction is the formation of 0.1 µmol of product in 20 min at the indicated temperature. Assays were performed in Buffer P containing 0.05 mM PLP. Activity in the beta-elimination reaction () was measured by a spectrophotometric assay coupled with lactate dehydrogenase at temperatures up to 50 °C(24) . Because lactate dehydrogenase was inactivated above 50 °C, the assay between 50 and 70 °C was allowed to proceed in a water bath for 5 min, stopped by addition of 1 N HCl, and neutralized with NaOH. Pyruvate concentration was then determined by the addition of lactate dehydrogenase and NADH at 37 °C. Activity in the beta-replacement reaction () was measured by a direct spectrophotometric assay (22) . The concentration of L-serine (100 mM) used for assays was saturating because either activity gave the same rate with 50 mM, 100 mM, and 200 mML-serine at 14 and 50 °C.


RESULTS

Effects of Temperature on the Absorption Spectrum of the beta(2) Subunit

The absorption spectra of the beta(2) subunit display two distinct absorption maxima at 410 and 336 nm at temperatures between 17 and 70 °C (Fig. 1B). These absorption peaks at 24.5 °C have been attributed to the ketoenamine form of the internal aldimine (II in Fig. 1A) and to the enolimine tautomer (I in Fig. 1A), respectively(10, 25) . As the temperature is increased, the initially higher absorbance at 410 nm decreases, and the absorbance at 336 nm increases. Between 17 and 50 °C, these temperature-dependent spectral changes are fully reversible, and the spectra exhibit a single isosbestic point at 355 nm (Fig. 1B) consistent with the interconversion of two species (e.g.I and II in Fig. 1A). The loss of activity above 50 °C (see below) is also reversed by cooling. Enzyme heated to 50 °C for 50 min and then cooled had the same activity as an unheated control. Above 50 °C, the isosbestic point is lost, and the change in absorbance at 336 nm is greater than that at 410 nm. Parallel studies of the temperature dependence of the absorption spectra of a model Schiff base between PLP and L-serine show a smaller decrease in absorbance at 410 nm and an increase in absorbance at 336 nm (see below).

Fig. 2A shows plots of absorbance data versus temperature for the beta(2) subunit and for the model Schiff base. The absorbance changes of the beta(2) subunit occurring below 60 °C were analyzed assuming a simple two-state equilibrium between two forms of the protein, a low temperature form, N, and a high temperature form, I. The equilibrium constant for this reaction, K, can be calculated from the temperature dependence of the absorbance of the solution at any wavelength using :


Figure 2: Effect of temperature on spectral properties and activities of the beta(2) subunit. A, plot of normalized extinction coefficients versus temperature in °C for the absorbance changes at 410 nm (box) using Method 1 (see Fig. 1B), and additional data were collected (circle) using Method 2. The deviation of the data obtained by the two methods at higher temperatures may result from the fact that the enzyme used for Method 1 was expressed by a plasmid encoding the trpB gene (21) , whereas the enzyme used in Method 2 was prepared by heat precipitation of the alpha subunit from the alpha(2)beta(2) complex(22) . The data were fitted to and as described under ``Results.'' The derived value of T(m) = 43.2 ± 0.8 is given. The changes in the mM extinction at 410 nm (up triangle) for the model Schiff base of L-serine and PLP (0.05 mM PLP with 50 mML-serine in Buffer P) were determined from spectra recorded at different temperatures as described for the enzyme in Fig. 1and are plotted in a similar way. The dashed lines show pre- and post-transition base-lines. B, the ellipticity changes at 410 nm (circle) are plotted versus temperature in °C and fitted to and as described under ``Results.'' The derived T(m) value is given. C, the ratios of the observed initial rate of the beta(2) subunit in beta-elimination (box) () and beta-replacement (circle) () reactions (Fig. 3A) to the predicted rates (dashed lines in Fig. 3B) are plotted versus temperature in °C. D, the logarithm of the fluorescence emission at 510 nm upon excitation at 420 nm is plotted versus temperature in °C for the beta(2) subunit alone (circle), beta(2) subunit + L-serine (up triangle), and model Schiff base (box). Intensities are plotted on a semi-logarithmic scale versus temperature in °C in order to show all of the data on the same chart and to simplify the temperature dependence of the fluorescence of the native protein. Curves were fitted to the untransformed data as explained in the text. Intensities were measured on solutions in Buffer P containing 61 µM beta(2) subunit alone, 3.7 µM beta(2) subunit in the presence of 0.1 ML-serine, or 0.05 mM PLP in the presence of 50 mML-serine and were normalized to 3.7 µM PLP. Measurements on the beta(2) subunit alone and of the model Schiff base of PLP with L-serine were made on a single solution of each after equilibration for 10-15 min at each temperature. Each measurement on the beta(2) subunit in the presence of L-serine was made on a separate solution after equilibration at the indicated temperature for 10 min. Two sets of data were collected in the presence of L-serine. One set was collected in the presence 50 µM PLP; the other was collected in the absence of added excess PLP. Each data set gave the same T(m). The temperature of the beta(2) subunit alone was gradually increased up to 73 °C and then cooled to 4.5 °C. The finding that the fluorescence emission of the previously heated beta(2) subunit was 86% that of the unheated enzyme at 4.5 °C demonstrates that the thermal transition is largely reversible. The derived values of T(m) are given and are marked on the curves by small arrows.




Figure 3: Effect of temperature on the activities of the beta(2) subunit. A, the initial rates of the beta(2) subunit in beta-elimination (box) () and beta-replacement (circle) () reactions are plotted versus temperature in °C. B, the data from A are plotted as log of activity versus the reciprocal of the absolute temperature in K. The low temperature data were fitted to the Arrhenius equation () as described under ``Results,'' and the derived constants were used to predict rates at higher temperatures (dashed lines). These values together with the measured rates were used to calculate the values of alpha(T) for both reactions, which were then fitted to to derive the values of T(m) given in Fig. 2C. and were combined to predict the temperature dependence of the measured activities (solid lines in Fig. 2C).



where A(T) is the absorbance of the solution and A(n)(T) and A(i)(T) are the absorbancies of the low and high temperature forms of the protein at temperature T, where T is absolute temperature in K(26) . The temperature dependence of A(i)(T) could not be observed, because it was masked by the more extensive changes occurring above 60 °C ( Fig. 1and Fig. 2A). For a two-state unfolding mechanism, the dependence of K on temperature T is given by :

where DeltaG(T), DeltaH, and DeltaS are the changes in free energy, enthalpy, and entropy of the reaction and T(m) is the midpoint and where DeltaG(T) = 0, assuming DeltaC(p) = 0. A control experiment with the model Schiff base of PLP with L-serine showed a linear temperature dependence (Fig. 2A). The temperature-dependent decreases in absorbance at 410 nm and increases at 336 nm were much smaller than those observed with the enzyme-bound PLP. Absorbance changes at 410 nm were fitted to and using the PC-MLAB program (Civilized Software, Bethesda, MD) to derive values of DeltaH and T(m) using the assumption that the absorbance of the native protein A(n)(T) showed the same linear temperature dependence as that of the model Schiff base and that the high temperature form of the protein A(i)(T) showed a parallel linear dependence. The fitted curve (T(m) = 43.2 ± 0.5 °C; DeltaH =180 ± 30 kJ/mol) is shown in Fig. 2A.

Effect of Temperature on the Circular Dichroism Spectrum of the beta(2) Subunit

The binding of PLP to the beta(2) subunit induces a positive ellipticity band at 415 nm due to the asymmetric orientation on the enzyme of the otherwise optically inactive chromophore(12, 13) . Remeta et al.(15) have recently found that the ellipticity band at 415 nm of the beta(2) subunit in the alpha(2)beta(2) complex disappears with heating at a low temperature transition (T(m) = 46.4 °C) and that the beta(2) subunit alone exhibits a similar transition.^2 Measurements of the ellipticity of the beta(2) subunit as a function of temperature detected a decrease in ellipticity at 410 nm (Fig. 2B) but very little ellipticity at 336 nm at any temperature. The ellipticity changes at 410 nm were analyzed assuming a simple two-state equilibrium between two forms of the protein as above and fit to and . Adequate fits were obtained assuming that the ellipticities of the two forms of the protein were independent of temperature (Fig. 2B). The complete loss of ellipticity at 410 nm indicates that the observed temperature-induced transition (T(m) = 41 °C; DeltaH = 150 ± 40 kJ/mol) results in loss of asymmetric binding of PLP. However, PLP must remain bound to the enzyme at 60 °C, because the absorbance maximum is at 336 nm, whereas free PLP in solution has an absorbance maximum at 388 nm (see below).

Effects of Temperature on the Activities of the beta(2) Subunit

Fig. 3A shows the effect of temperature on the activities of the beta(2) subunit in the beta-elimination () and beta-replacement () reactions. The initial rate in each reaction was maximum at 55 °C and 5-6-fold higher than the rate at 37 °C, the usual assay temperature. The rates are measured under zero order conditions where [S] K(m). The observed rates were linear below 45 °C. However, the rates above 45 °C showed progressive decrease with time, indicating enzyme inactivation during the assay. The observed decreases in rate result from a decrease in V(max), not from a increase in K(m), because the enzyme is saturated by L-serine under the assay conditions for both reactions (see ``Experimental Procedures'').

The temperature dependence of k is given by the Arrhenius equation ():

where E is the energy of activation of the reaction and T is absolute temperature in K. Fig. 3B shows Arrhenius plots of the rates of both reactions. (^3)Both plots are linear below 45 °C as predicted and yield DeltaE = 29.2 ± 0.6 kcal/mol for the beta-replacement reaction and DeltaE = 31 ± 0.6 kcal/mol for the beta-elimination reaction. Above 45 °C the measured rates decrease markedly from those predicted by linear extrapolation of the plots. If the enzyme is in an equilibrium between an active, low temperature form (N) and an inactive, high temperature form (I), then the fraction of the protein (alpha) that is in the active form at a given temperature is given by :

and the equilibrium constant for the reaction between N and I is given by :

The temperature dependence of (K)T is again given by , where T is absolute temperature in K. The low temperature data in Fig. 3A were fitted to the Arrhenius equation using PC-MLAB, and the derived constants were used to predict rates at higher temperatures (dashed lines in Fig. 3B). These values together with the measured rates were used to calculate the values of alpha(T) for both reactions, shown in Fig. 3B, which were then fitted to to derive the values of T(m), shown in Fig. 2C. and were combined to predict the temperature dependence of the measured activities (Fig. 3B, solid lines) and the rate ratios (Fig. 2C, solid and dashed lines).

Effects of Temperature on the Fluorescence Emission of the beta(2) Subunit in the Absence and Presence of L-Serine

PLP bound to the beta(2) subunit exhibits a weak fluorescence emission with a peak at 505 nm(27) . The fluorescence emission intensity increases markedly upon addition of L-serine due to formation of E-Ser(27) . Fig. 2D shows a semi-logarithmic plot of the effects of temperature on the fluorescence emission intensity at 510 nm (uncorrected for inner filter effect) of the beta(2) subunit in the presence and the absence of 0.1 ML-serine and of the model Schiff base of PLP with L-serine. The fluorescence intensities of the native (pretransition) forms of the protein and of the model Schiff base exhibit marked temperature dependence as described previously for base-line trends(28) . The base-line data for the enzyme show linear dependence on temperature below 40 °C in a semi-logarithmic plot (Fig. 2D), whereas the behavior of the model Schiff base of PLP with L-serine is linear throughout the temperature range, as would be expected from Equation 21 in the work of Eftink(28) . This linear dependence was used to extrapolate the behavior of the native proteins in the presence and the absence of L-serine into their transition zones. The fluorescence of the high temperature forms of the proteins were assumed to parallel that of the model Schiff base, and two state transition curves were fitted to and (26) using the PC-MLAB program. The fitted curves and the derived values of T(m) are shown in Fig. 2D.

Effect of Temperature on the Integrity of the Internal Aldimine Bond in the beta(2) Subunit

It is important to determine whether the enzyme-bound PLP at 60 °C exists as the enolimine species in a nonpolar environment (I in Fig. 1A) or as PLP in a nonpolar environment with its carbonyl group free. Studies of the effects of polarity on the absorption spectra of PLP analogs using dioxane/water mixtures have shown that reducing the polarity results in a blue shift of about 30-50 nm in the absorbance maximum(29, 30, 31) . The absorption spectra of PLP in dioxane/water mixtures (Fig. 4) show that PLP has an absorption maximum of 390 nm in water and 350 nm in 98% dioxane. Our finding that free PLP in a nonpolar environment absorbs at a significantly higher wavelength (350 nm) than the species observed at 60 °C (336 nm) indicates that the enzyme-bound species at 60 °C is not free PLP but is an internal aldimine linkage in a nonpolar environment.


Figure 4: Absorption spectra of PLP in dioxane/water mixtures. PLP (10 mM) was diluted 50-fold into dioxane/water mixtures. The final solutions contained 0.2 mM PLP in the indicated percentage of dioxane by volume.



To establish definitively that PLP is bound to the beta(2) subunit at 60 °C in the form of an internal aldimine, we have reconstituted the apo beta(2) subunit with [4`-^3H]PLP as described under ``Experimental Procedures'' and have treated the enzyme with sodium borohydride at 15 or 60 °C as described(20) . The absorption spectra of both treated enzymes exhibited peaks at 318 nm, showing that reduction of PLP had occurred. However, this result could not distinguish whether free PLP was reduced to pyridoxine 5`-phosphate or whether the internal aldimine was reduced to 5`-phosphopyridoxyl lysine because these products have similar absorption spectra. Acid precipitation of the proteins followed by analysis of the radioactivity in the supernatant solutions (Table 1) showed that more than 80% of the [4`-^3H]PLP became covalently attached by reduction of the beta(2) subunit at either 15 or 60 °C. Thus the PLP must be bound as an internal aldimine at 60 °C and must be reduced by sodium borohydride. The low but significant amount of radioactivity in the supernatants of both sodium borohydride treated samples (Table 1) may be due to dissociation of some of the cofactor before reduction or to binding of a radioactive impurity that does not form an internal aldimine.




DISCUSSION

The PLP coenzyme has been used previously as a sensitive chromophoric probe to investigate the structure and function of many PLP-dependent enzymes. However, there have been very few studies to our knowledge of the effects of temperature on the spectroscopic properties of PLP-dependent enzymes, of PLP itself, or of PLP derivatives. (^4)Recent studies from Ginsburg's group(15, 16, 17) ^2 and the studies reported here show that PLP serves as a useful probe of the effects of temperature on the structure and function of the tryptophan synthase beta(2) subunit and alpha(2)beta(2) complex.

Evidence for a Thermally Induced Reversible Conformational Transition

Our results provide evidence for a reversible thermal transition of the beta(2) subunit that occurs at a much lower temperature than the major unfolding transition at 80 °C (15, 16, 17, 18) .^2 This low temperature transition alters the absorption spectrum of enzyme-bound PLP ( Fig. 1and Fig. 2A) and leads to loss of fluorescence emission of the internal and external aldimines at 510 nm (Fig. 2D) and of ellipticity at 410 nm (Fig. 2B) and to loss of activity (Fig. 2C and 3, A and B). Temperature affects the activities of the beta(2) subunit in beta-elimination and beta-replacement reactions ( and ) in two ways; the activities first increase in rate with temperature until maximal rates are achieved at 55 °C and then decrease due to thermal inactivation (Fig. 3A). In the region prior to inactivation (below 45 °C), the data are described by the Arrhenius equation () and can be fit to the straight lines shown in Fig. 3B. Analysis of the data (Fig. 2C) shows that the beta(2) subunit reversibly loses one-half of its activity under assay conditions at a much lower temperature (T(i) = 52 °C) than the temperature (T(i) = 77 °C) at which the beta(2) subunit undergoes irreversible inactivation(18) .

The values of T(m) for the absorption and circular dichroism changes for the internal aldimine forms (in the absence of L-serine) shown in Fig. 2(A and B) vary from 40-43 °C, (^5)whereas the values of T(i) for reversible inactivation (Fig. 2C) and of T(m) for fluorescence changes for the external aldimine forms (in the present of L-serine) (Fig. 2D) are significantly higher (49-53 °C). Although formation of the external aldimine forms in the presence of L-serine appears to stabilize the enzyme, (^6)the spectral changes and inactivation observed in the presence and the absence of L-serine probably reflect the same transition. Our results also indicate that the species of the cofactor that absorbs at 410 nm exhibits optical activity at 410 nm and emits fluorescence at 510 nm upon excitation at 420 nm is the catalytically competent species.

The observed low temperature transition does not result in dissociation of the internal aldimine between PLP and the -amino group of Lys-87, because treatment with sodium borohydride results in covalent attachment of PLP to the protein at both 15 and 60 °C (Table 1). Our results indicate that PLP is bound to the inactive conformer of the beta subunit as the enolimine tautomer of the internal aldimine (I in Fig. 1A), a neutral species that prefers a nonpolar environment(3, 4) .

Structure of the Inactive Conformer That Results from the Low Temperature Transition

Information on the structure of the inactive conformer of the beta(2) subunit is available from studies using differential scanning calorimetry, circular dichroism, and UV spectroscopy(15, 16, 17) ^2 and from the results presented here. The differential scanning calorimetry profiles of the holo beta(2) subunit exhibit a reproducible broad, low temperature endotherm (T(m) = 47 °C; DeltaH = 180 kJ/mol), whereas major unfolding, which disrupts 70% of the secondary structure, occurs between 74 and 82 °C(15) . The low temperature endotherm is consistent with a small conformational transition. The single tryptophan in each beta subunit (Trp-177), which is buried in the hydrophobic core of the N-terminal domain of each beta subunit, is a useful intrinsic probe for monitoring thermally induced unfolding in the alpha(2)beta(2) complex (15) and the isolated beta(2) subunit.^2 Thermally induced perturbations in Trp and Tyr environments were independently monitored using second derivative UV absorption spectroscopy of tryptophan synthase beta(2) subunit^2 and alpha(2)beta(2) complex(15) . The results revealed perturbations of Trp-177 but not of tyrosyl residues in beta chains in the low temperature transition(15) . Perturbation of Trp-177 may result from solvent exposure or change in the polarity of the environment. The absence of perturbation of tyrosyl residues suggests that partial unfolding and solvent exposure does not occur and supports a change in polarity of the environment of Trp-177 resulting from a small conformational transition.

Analysis of the spectral properties of the bound PLP show that both a change in the tautomeric form of the internal aldimine ( Fig. 1and 2A) and loss of the ellipticity at 410 nm (Fig. 2B) occur in the low temperature transition. Thus, the low temperature transition involves several reversible changes in the structure of the beta subunit: 1) perturbation in the environment of Trp-177, 2) change in the environment of the PLP, and 3) a conformational transition that contributes to the low temperature endotherm. This transition must reflect a small change in structure or a conformational change because it does not break the internal aldimine of PLP (Table 1) or result in exposure of the buried tyrosine residues in the beta subunit.^2 The low temperature transition is clearly biologically relevant because it results in loss of catalytic activity. An additional important conclusion is that the species of PLP that absorbs at 410 nm, exhibits optical activity at 410 nm, and emits fluorescence at 510 nm upon excitation at 420 nm is the catalytically active form of the cofactor in the beta(2) subunit.


FOOTNOTES

*
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
To whom correspondence should be addressed: NIH, Bldg. 8, Rm. 2A07, Bethesda, MD 20892. Tel.: 301-402-1495; Fax 301-402-0240.

(^1)
The abbreviation used is: PLP, pyridoxal 5`-phosphate.

(^2)
D. P. Remeta, E. W. Miles, and A. Ginsburg, manuscript in preparation.

(^3)
The measured rates are proportional to k, the first order rate constant for the appearance of product, because the enzyme was assayed at saturating concentrations of the substrate, L-serine (see ``Experimental Procedures'').

(^4)
Metzler's group (32) has measured absorption spectra of 5-deoxypyridoxal over the temperature range 2.5-68 °C in acidic, neutral, and basic conditions. Band shape analysis of spectra at pH 5.8 shows that the proportion of the dipolar ion is relatively constant, whereas the ratio of hydrate to uncharged species decreases with increasing temperature. Parallel studies with Schiff base derivatives of PLP have not been carried out previously. Schiff base derivatives, which do not form hydrates, might be expected to be less affected by temperature as shown by our results in Fig. 2.

(^5)
Some of this variation may be due to experimental errors. The precise determination of the thermodynamic parameters of any conformational transition requires the accurate measurement of the temperature-dependent behaviors of both the high and low temperature forms of the protein, so that they can be extrapolated into the transition zone. This allows a good estimate of the extent of reaction(26) . In many of the experiments presented herein, it was not possible to follow the behavior of the inactive beta(2) subunit at high temperatures, because the data were obscured by the overlapping unfolding transition, which occurs around 77 °C. In the absence of any better information, high temperature changes were assumed to parallel those of the low temperature form of the protein or of the model PLP Schiff base with L-serine. This uncertainty increases the experimental error in the fitted values of T(m).

(^6)
One discrepant observation is that the T(m) for fluorescence changes in the absence of added L-serine (47 °C) is only 2 degrees lower than that in the presence of L-serine (49 °C) (Fig. 2D). A possible explanation for this result is that the low level of fluorescence measured in the absence of added L-serine results from a small population of enzyme-bound L-serine (<5%) that was not removed during purification of the enzyme.


ACKNOWLEDGEMENTS

We thank Dr. David E. Metzler for helpful comments on an early version of this manuscript. We are grateful to Drs. Ann Ginsburg and David P. Remeta for useful advice, unpublished information, and for generous assistance with the use of their Hewlett Packard model 8450 spectrophotometer.


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