©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
Activation-induced Association of a 145-kDa Tyrosine-phosphorylated Protein with Shc and Syk in B Lymphocytes and Macrophages (*)

(Received for publication, June 7, 1995; and in revised form, September 21, 1995)

Mary T. Crowley (1)(§)(¶) Stacey L. Harmer(§) (2)(**) Anthony L. DeFranco (1) (2)(§§)

From the  (1)G. W. Hooper Foundation Departments of Microbiology and Immunology and the (2)Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143-0552

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

Engagement of many cell surface receptors results in tyrosine phosphorylation of an overlapping set of protein substrates. Some proteins, such as the adaptor protein Shc, and a frequently observed Shc-associated protein, p145, are common substrates in a variety of receptor signaling pathways and are thus of special interest. Tyrosine-phosphorylated Shc and p145 coprecipitated with anti-Shc antibodies following B cell antigen receptor (BCR) cross-linking or interleukin-4 (IL-4) receptor activation in B cells, and after lipopolysaccharide (LPS) treatment or IgG Fc receptor (FcR) cross-linking in macrophages. In the case of BCR stimulation, we have shown that this represented the formation of an inducible complex. Furthermore, in response to LPS activation or FcR cross-linking of macrophages and BCR cross-linking (but not IL-4 treatment) of B cells, we observed a similar tyrosine-phosphorylated p145 protein associated with the tyrosine kinase Syk. We did not detect any Shc associated with Syk, indicating that a trimolecular complex of Shc, Syk, and p145 was not formed in significant amounts. By several criteria, the Syk-associated p145 was very likely the same protein as the previously identified Shc-associated p145. The Syk-associated p145 and the Shc-associated p145 exhibited identical mobility by SDS-polyacrylamide gel electrophoresis and identical patterns of induced tyrosine phosphorylation. The p145 protein that coprecipitated with either Shc or Syk bound to a GST-Shc fusion protein. In addition, a monoclonal antibody developed against Shc-associated p145 also immunoblotted the Syk-associated p145. The observations that p145 associated with both Shc and Syk proteins, in response to stimulation of a variety of receptors, suggest that it plays an important role in coordinating early signaling events.


INTRODUCTION

Receptors for antigens, cytokines, and growth factors utilize tyrosine phosphorylation of proteins to initiate and propagate intracellular events that result in cellular responses. As many of these receptors lack intrinsic tyrosine kinase activity, this increase in cellular phosphorylation can result from the recruitment and activation of cytoplasmic tyrosine kinases including Syk, ZAP-70, Src family tyrosine kinases(1) , and JAK family tyrosine kinases(2) . Targets of these kinases include enzymes that generate second messengers, regulators of Ras and other Ras-like G proteins, transcription factors, and a variety of other proteins that are believed to play a role in receptor signaling(3) .

Recently, attention has focused on the Shc protein, which is a ubiquitously expressed adaptor protein that is tyrosine-phosphorylated following stimulation of B or T lymphocyte antigen receptors (BCR (^1)and TCR)(4, 5, 6) , growth factor receptors(7) , and cytokine receptors(8, 9, 10, 11) . The SHC1 gene encodes the two major Shc isoforms, p52and p46. These two proteins are produced by utilization of two in-frame translation initiation sites. Each Shc isoform contains a C-terminal SH2 domain, a proline-rich central domain with multiple collagen-alpha1-type repeats, and at the N terminus, a recently identified phosphotyrosine interaction domain(12, 13, 14, 15) . All of these domains are likely to be important for mediating protein-protein interactions(12) . Phosphorylation at Tyr-317 of the central domain of Shc directs binding of the Grb-2bulletSOS-1 complex to Shc via the SH2 domain of Grb-2(16) . By virtue of this association, Shc has been implicated in Ras activation. The localization of SOS-1 to the plasma membrane is necessary for the activation of Ras(17) . Although Shc is not itself a membrane protein, it can bind to tyrosine-phosphorylated activated IL-2 receptor(18) , erythropoeitin receptor(10) , or TCR(5) . Presumably this binding, coupled with Grb-2 binding to phosphorylated Shc, would direct Grb-2bulletSOS complexes to the membrane following receptor activation. Other reports have implicated Shc in Ras activation in non-hematopoietic cells as well(19, 20) .

In different cell types activated by a variety of stimuli, Shc also associates with a highly tyrosine-phosphorylated protein of approximately 145 kDa(4, 11, 21, 22) . Depending upon the cell type analyzed, this protein has been referred to as p140, p145, or p150. It can appear as a single band or as several closely spaced bands. These Shc-associated proteins may well be the same protein or very similar isoforms of the same protein. In this report, we used BCR-stimulated B lymphocytes as a model system to demonstrate that the p145bulletShc association is an inducible event and that complex formation and maintenance correlates with levels of tyrosine phosphorylation of these proteins. In examining responses via other signaling receptors, we found association of tyrosine-phosphorylated p145 with Shc in response to IL-4 treatment of B lymphocytes and FcR cross-linking or LPS treatment of macrophages. To address which tyrosine kinases may phosphorylate Shc and p145, we looked for direct association of these substrates with specific kinases. We found that Syk kinase coprecipitated with the p145 protein following most but not all of the same stimuli that induced p145 to associate with Shc. These observations suggest that p145 may have an important role in coordinating signaling pathways from a number of receptors.


MATERIALS AND METHODS

Reagents

Goat anti-mouse IgM antibodies were obtained from Jackson Immunological Research Laboratories (West Grove, PA). Murine IL-4 was purchased from Genzyme (Cambridge, MA). Antibodies against Shc were obtained from Transduction Laboratories (Lexington, KY). The 4G10 anti-phosphotyrosine antibody was prepared and used as described(23) . The 2.4G2 anti-FcR rat monoclonal antibody was purchased from Pharmingen (San Diego, CA). The mouse anti-rat immunoglobulin kappa chain monoclonal antibody, MAR18.5, was obtained from ATCC and was used to cross-link 2.4G2. Anti-Syk antibody was produced by immunizing rabbits with a GST fusion protein containing amino acids 259-333 of murine Syk. (^2)Horseradish peroxidase (HRP)-conjugated sheep anti-mouse IgG antibody, Pro-Mix [S]methionine and [S]cysteine cell labeling mix, and [-P]ATP were obtained from Amersham Corp. HRP-conjugated sheep anti-rabbit IgG antibody was obtained from Boehringer Mannheim. Protein A-Sepharose was from Zymed Laboratories (South San Francisco, CA). LPS from Salmonella minnesota was obtained from Difco Laboratories (Detroit, MI) or List Biological Laboratories (Campbell, CA). Phorbol 12-myristate 13-acetate and chicken serum were purchased from Sigma. Pervanadate solution was prepared as described(24) .

Cell Culture

Cell lines used in this study (murine pre-B and B cell lines 70Z/3, WEHI-231, Bal-17, 2PK3; human B cell lines Daudi and Ramos and murine macrophage cell line RAW 264.7) were cultured in RPMI 1640 medium supplemented with 5% fetal calf serum, 2 mM sodium pyruvate, 1 mM glutamine, and 50 µM beta-mercaptoethanol. Chicken B cell lines were cultured in RPMI 1640 medium supplemented with 10% fetal calf serum and 1% chicken serum. Splenic resting B cells were prepared by antibody- and complement-mediated lysis of T cells, followed by Percoll density gradient centrifugation as described previously(25) .

Anti-p145 Monoclonal Antibody Producing Hybridomas

p145 protein in Shc immunoprecipitates was separated from other precipitated proteins by SDS-PAGE. Purified p145 protein was excised from gels and mixed with RIBI adjuvant as per the manufacturer's protocol (RIBI, Hamilton, MT; BALB/c mice were injected intraperitoneally with antigen-adjuvant mixture and boosted with antigen at 7 and 14 days. Fusions were performed using spleen cells and fusion partner Sp2/0-Ag14. Hybridoma supernatants were screened for reactivity with p145 protein by immunoblotting as described below.

Metabolic Labeling of Cell Proteins

WEHI-231 cells were washed twice and then resuspended (5 times 10^6 cells/ml) in cysteine-free, methionine-free RPMI 1640 medium supplemented with 10% dialyzed fetal calf serum. Cells were starved for 1 h at 37 °C. Cells were then resuspended (5 times 10^6 cells/ml) in fresh labeling medium containing [S]methionine and [S]cysteine (50 µCi/ml). Cells were labeled for 2-4 h and stimulated as described below.

Cell Stimulation, Immunoprecipitation, and Immunoblotting

Murine B lymphocytes (5 times 10^6 cells/ml in culture medium) were stimulated at 37 °C for 3-5 min (unless otherwise indicated) with medium only, goat anti-IgM antibodies (15 µg/ml, unless otherwise indicated), or IL-4 (100 units/ml). Cell suspensions were then diluted with cold phosphate-buffered saline containing 1 mM sodium vanadate (PBS/vanadate), pelleted by centrifugation, and resuspended in ice cold lysis buffer consisting of 1% Triton X-100, 50 mM HEPES buffer, pH 7.4, 150 mM NaCl, 10% glycerol, 1.5 mM MgCl(2), 1 mM EGTA, 100 mM NaF, 1 mM phenylmethylsulfonyl fluoride, 1 mM Na(3)VO(4). For FcR stimulations of macrophages, 1 times 10^7 cells/ml suspended in culture medium were incubated on ice for 30 min with 2.4G2 antibody (40 µg/ml). Cells were then warmed to 37 °C and treated with or without cross-linking antibody (10 µg/ml MAR18.5) for 10 min. Cells were then washed and lysed as described above. For LPS responses in macrophages, LPS (5 µg/ml) was added to cultures of adherent, confluent RAW 264.7 cells for 5 min. Following stimulation, cells were scraped off the plate and the cell suspensions were treated as described above. Chicken B cells (1 times 10^7/ml) in culture medium plus 10 mM HEPES, pH 7.4, were incubated at 37 °C for 4 min with or without 400 µM pervanadate(24) . Following stimulation, cells were washed by centrifugation once with ice-cold PBS/vanadate. Cell pellets were lysed in ice cold lysis buffer containing 1% Triton X-100, 20 mM Tris, pH 8.0, 137 mM NaCl, 10% glycerol, 2 mM EDTA, 1 mM phenylmethylsulfonyl fluoride, and 1 mM Na(3)VO(4). Solubilized cell lysates were centrifuged (14,000 times g for 10 min) to remove nuclei and insoluble cellular debris.

Immunoprecipitations, SDS-PAGE, and immunoblotting were carried out as described previously(26) . Briefly, lysates were precleared by incubation with 25 µl of protein A-Sepharose for 1 h at 4 °C. Precleared lysates were incubated with anti-Shc polyclonal antibody, used at a concentration of 1 µg of antibody/mg of cell lysate protein. Anti-Syk polyclonal antiserum was used at a concentration of 5 µl of antiserum/mg of cell lysate protein. For anti-phosphotyrosine immunoblotting, 4G10 hybridoma supernatant was used at a 1:5 dilution. Alternatively, anti-Shc monoclonal antibody was used at 1 µg/ml or anti-Syk antiserum was used at a 1:1000 dilution. Blots were incubated with HRP-conjugated secondary antibody (1 µg/ml in TBST) for 30-60 min. Bands were visualized using the Renaissance chemiluminecence detection system (DuPont).

GST Fusion Protein Blotting

The full-length sequence of the p52 isoform of Shc was generated by PCR from a Shc cDNA clone (kind gift of P. G. Pelicci, University of Perugia, Italy). The identity of the resulting fragment was verified by sequencing using standard techniques and the fragment was ligated into the polylinker of the bacterial expression vector pG-IKS (a generous gift of Dr. Khoi Lee, UCSF). GST fusion proteins encoded by this vector contain a 5-amino acid recognition element for the catalytic domain of bovine heart muscle kinase, which was used to label the fusion protein with [-P]ATP. Purified GST-Shc fusion proteins were produced as described previously(27) . Shc and Syk immunoprecipitations were resolved by SDS-PAGE and transferred to nitrocellulose membranes as described above. Membranes were denatured and then renatured with 6 M guanidine hydrochloride, blocked with milk and incubated with 250 times 10^3 cpm/ml radioactively labeled GST-Shc overnight as described(13) . After hybridization, membranes were washed and then exposed to film. Bound GST-Shc was removed from membranes before reprobing with anti-phosphotyrosine antibodies. Membranes were first incubated in stripping solution (0.1 M 2-mercaptoethanol, 2% SDS, 62.5 mM Tris, pH 6.7) at 50 °C for 30 min. Membranes were then washed, reblocked, and subjected to anti-phosphotyrosine immunoblotting as described above.


RESULTS

BCR-induced Association of Shc with p145

Recent reports have demonstrated that stimulation through a variety of receptors in different cell types results in tyrosine phosphorylation of the signaling adapter protein Shc and the association of Shc with a 145-kDa tyrosine-phosphorylated protein. For example, BCR cross-linking in B lymphocytes(4) , macrophage colony-stimulating factor treatment of myeloid cells(21) , and IL-3, Steel factor, and erythropoietin treatment of responsive hematopoietic stem cell lines (22) all induce the appearance of a 140-150-kDa tyrosine-phosphorylated protein in Shc immunoprecipitates. This could reflect induced tyrosine phosphorylation of p145, induced association of p145 with Shc, or both.

In order to better understand the nature of this complex and, hence, its role in B cell responses, it was important to determine first whether Shcbulletp145 was present as a complex in unstimulated B cells or whether the association of p145 and Shc required stimulation of the cells. Therefore, we immunoprecipitated Shc from either resting or BCR-stimulated S-labeled cells (Fig. 1A). Biosynthetically labeled p145 only coprecipitated with Shc after cell stimulation, demonstrating that the association between these proteins was inducible. Other Shc-associated proteins were also observed in this way. The prominent 70-kDa species visible only in stimulated lysates was detected by anti-mouse Ig immunoblotting and therefore is likely to be endogenous immunoglobulin µ heavy chain that was immunoprecipitated by the stimulating antibody. In addition to p145, proteins of 40, 45, 100, and 110 kDa coprecipitated with Shc at 15 and 30 min following stimulation. The identity of these bands was unknown, and none were recognized by anti-phosphotyrosine antibodies. However, anti-phosphotyrosine immunoblotting of the same samples showed the prominent doublet of Shc-associated tyrosine-phosphorylated proteins at 140 and 145 kDa (Fig. 1B). In addition, a weaker tyrosine-phosphorylated band of 150 kDa was sometimes seen. To confirm that the inducibly associated 140- and 145-kDa proteins were indeed the tyrosine-phosphorylated species observed, the S-labeled Shc immunoprecipitates were dissociated by treatment with 2% SDS and then reimmunoprecipitated with anti-phosphotyrosine antibody. The metabolically labeled protein doublet at 140-145 kDa was reprecipitated in this way (data not shown), indicating that these bands corresponded to the p145 tyrosine-phosphorylated proteins. We believe that these bands were all isoforms of the same protein as each of these proteins exhibited the same properties in all of the experiments reported here. The nature of the changes that caused differential migration are not known at this time. These bands retained their different mobilities after de-phosphorylation with calf intestinal phosphatase (data not shown), suggesting that some property other than phosphorylation was responsible for the differential mobility of these bands.


Figure 1: Activation-induced association of a 145-kDa, tyrosine-phosphorylated protein with Shc in B cells. WEHI-231 cells (5 times 10^7 cells/point) were labeled with [S]methionine and [S]cysteine for 4 h. Cells were stimulated for the indicated times during cell labeling such that all time points were harvested after 4 h of metabolic labeling. Shc immunoprecipitates were resolved by SDS-PAGE, transferred to nitrocellulose, exposed to film (A), and subsequently immunoblotted with anti-phosphotyrosine antibody (B). The position of the S-labeled p145 doublet (p140, p145) is indicated in A, and the positions of the tyrosine-phosphorylated Shc and p145 doublet proteins are indicated in B. The Shc proteins were not readily apparent in these labeling experiments (A), although they can be detected by anti-Shc immunoblotting. The anti-phosphotyrosine immunoblotting signals were comparable to those from unlabeled cells. In panel B, the band below p52 was also seen with protein A-Sepharose and the sheep anti-mouse Ig secondary reagent and hence is not specifically immunoprecipitated or tyrosine-phosphorylated. The p46 form of Shc is concealed by this background band. The migration positions of molecular weight markers are indicated.



The Formation of the Shcbulletp145 Complex and Its Persistence in the Cell Were Correlated with Tyrosine Phosphorylation

We also examined the kinetics of the Shcbulletp145 complex formation to determine whether this was a transient response or a prolonged signaling event following BCR stimulation (Fig. 1). The phosphotyrosine content of Shc and p145 increased rapidly and then decreased sharply by 30 min after stimulation. This response peaked at 8-10 min (data not shown). There was only faintly detectable tyrosine-phosphorylated p145 associated with Shc by 90 min after stimulation. The association of p145 with Shc as detected with biosynthetically labeled protein declined over a similar time course (Fig. 1A), except that the sensitivity of detection by metabolic labeling was less so the small amount of complex remaining at 90 min could only be detected by anti-phosphotyrosine immunoblotting. The correlation observed between tyrosine phosphorylation and association was consistent with a requirement for tyrosine phosphorylation of Shc and/or p145 proteins for the complex to remain intact. As Shc has two domains capable of interacting with other tyrosine-phosphorylated proteins, it is likely that BCR stimulation induced the tyrosine phosphorylation of p145 and this in turn led to Shc binding. Indeed, it was recently reported that p145 isolated from fibroblasts must be tyrosine-phosphorylated for in vitro association with Shc(13) .

Syk Kinase Association with p145

The induction of tyrosine phosphorylation of signaling proteins by BCR cross-linking requires the activities of intracellular tyrosine kinases. As the tyrosine kinases Syk and Lyn play important roles in signaling by the BCR(28) , we employed coimmunoprecipitation experiments to examine whether either of these kinases associated with Shc and/or Shc-associated p145 upon stimulation of B cells. The Syk- or Lyn-immunoprecipitated proteins were resolved by SDS-PAGE and immunoblotted with anti-phosphotyrosine antibody. In agreement with previous reports(29, 30) , we found that Syk was tyrosine-phosphorylated in response to BCR stimulation in B cells. Additionally, Syk immunoprecipitates (but not Lyn immunoprecipitates; data not shown) from BCR-stimulated B cells contained a p145 tyrosine-phosphorylated doublet (Fig. 2A). This Syk-associated doublet comigrated with the Shc-associated p145 doublet, suggesting they might be the same proteins. This suggested the possibility that Shc and Syk might interact to form a trimolecular complex with p145. However, there was no detectable Shc protein in the anti-Syk immunoprecipitates (Fig. 2B). Correspondingly, there was no Syk detected in the anti-Shc immunoprecipitates. Thus, the Sykbulletp145 complex did not include detectable amounts of Shc.


Figure 2: A complex of Syk with p145 but not Shc. Lysates from WEHI-231 cells stimulated through the antigen receptor (as in Fig. 1) were subjected to immunoprecipitations using anti-Shc or anti-Syk polyclonal antiserum (A). Shc complexes were isolated from 2 mg of lysate proteins, whereas 4 mg of lysate proteins were used for each of the anti-Syk immunoprecipitations. Isolated proteins were separated by SDS-PAGE, transferred to nitrocellulose, and immunoblotted with anti-phosphotyrosine antibody. The positions of Syk (72 kDa), Shc, and associated p145 are indicated. In addition, Shc and Syk immunoprecipitates from stimulated and unstimulated WEHI-231 cells were resolved by SDS-PAGE (B) and the possible presence of a coprecipitating component was assessed by immunoblotting in parallel with polyclonal anti-Syk antibodies (upper section) or monoclonal anti-Shc antibodies (lower section).



The p145 protein associated with Syk appeared to be the same as the p145 associated with Shc both by electrophoretic mobility and pattern of anti-phosphotyrosine blotting. To determine in a more direct manner whether these tyrosine-phosphorylated p145 proteins were indeed the same protein, we examined whether Shc could associate with p145 protein that was isolated by virtue of its association with Syk. For this purpose, we made use of previous observations that recombinant proteins containing full-length Shc fused to GST (GST-Shc) can interact with Shc-associated p145 resolved by SDS-PAGE and transferred to nitrocellulose(13) . Shc or Syk immunoprecipitates containing either Shc-associated or Syk-associated p145 respectively were resolved on SDS-PAGE, transferred to nitrocellulose, and probed with P-labeled GST-Shc proteins. As previously reported, GST-Shc proteins associated with p145 that had been coprecipitated with anti-Shc antibodies. Importantly, GST-Shc also associated with p145 that had been coprecipitated with anti-Syk antibodies (Fig. 3A). The signal associated with Syk-associated p145 was comparable to that seen with Shc-associated p145. This observation provides evidence that the p145 proteins that associated with Syk and with Shc were the same.


Figure 3: Syk-associated p145 can bind to Shc. Lysates from anti-IgM-stimulated or unstimulated BalI7 cells were subjected to immunoprecipitation with Shc- or Syk-specific antibodies (2 times 10^7 or 13 times 10^7 cells/point, respectively), resolved by SDS-PAGE, and transferred to a nitrocellulose membrane. Membranes were incubated with P-labeled GST-Shc fusion proteins, washed, and exposed to film for 11 h (A). After exposure, the same membranes were stripped, blocked, and immunoblotted with anti-phosphotyrosine antibody (B). Note that lysate from more cells was used for the anti-Syk immunoprecipitation to achieve similar amounts of tyrosine-phoshorylated p145 on the filters.



Finally, the Shc-associated and Syk-associated p145 proteins were immunologically related. Hybridomas were generated from spleen cells of mice immunized with purified, Shc-associated p145. Monoclonal antibodies were selected on the basis of reactivity with Shc-associated p145 by immunoblotting. One monoclonal antibody, 4U2, reacted in an immunoblot with both the Shc-associated, and Syk-associated p140, p145, and p150 phosphotyrosine containing proteins (Fig. 4). This monoclonal also reacted with a protein of 165 kDa, which did not appear to be tyrosine-phosphorylated. The identity of this protein is not known. The anti-p145 reactivity did not represent anti-phosphotyrosine immunoreactivity since this monoclonal antibody still immunoblotted p145 that was dephosphorylated by calf intestinal phosphatase (data not shown).


Figure 4: Monoclonal anti-Shc-associated p145 antibody immunoblots both Shc- and Syk-associated p145. Shc or Syk immunoprecipitates from anti-IgM-stimulated WEHI-231 cell lysates were separated by SDS-PAGE and transferred to nitrocellulose. Membranes were immunoblotted with anti-phosphotyrosine (P-Y) antibody (3 times 10^7 cells/lane) or 4U2, an anti-p145 antibody (13 times 10^7 cells/lane) and reactions were detected by use of an HRP-conjugated anti-mouse secondary antibody, which did not produce any signal by itself (not shown).



As p145 had not been previously reported to coprecipitate with Syk, it was important to demonstrate that the observed coimmunoprecipitation of Syk and p145 in activated B cells was not due to anti-p145 reactivity in the anti-Syk antiserum. To address this issue, we made use of a chicken B cell line (S1.10) in which Syk is not expressed because the syk genes had been disrupted by homologous recombination (31) . The knockout cells responded poorly to BCR stimulation, as previously reported, but they exhibited appreciable tyrosine phosphorylation of cellular proteins following treatment with the tyrosine phosphatase inhibitor pervanadate. In the parental Syk-expressing chicken B cell line, DT40, pervanadate treatment resulted in tyrosine phosphorylation of p145 and its coimmunoprecipitation with Shc and to a lesser extent with Syk (Fig. 5). The p145 protein appeared as a single band in these chicken cells, a pattern that has been reported previously in some mammalian cell types(13, 21) . In the pervanadate-treated syk cells (S1.10), phosphorylated p145 was coprecipitated by the anti-Shc antiserum but not by the anti-Syk antiserum (Fig. 5). Thus, anti-Syk antiserum only immunoprecipitated p145 from cells expressing Syk protein, demonstrating that the coprecipitation of the two proteins was not due to a cross-reactivity of the anti-Syk antiserum with p145 or some other p145-associated protein.


Figure 5: Immunoprecipitation of p145 with anti-Syk requires Syk expression. Chicken B cells DT40 (parental) and S1.10 (syk) were stimulated with or without added pervanadate solution (400 µM) for 4 min. Since total cellular phosphorylation was consistently lower in the syk cells as compared to the parental B cells, a greater amount of protein of the former was used for the immunoprecipitations to give similar phosphotyrosine signals. Cells were lysed and subjected to immunoprecipitation with anti-Shc antibody (0.6 times 10^8 cells or 1.8 times 10^8 cells/point) or anti-Syk antibody (1.5 times 10^8 or 4.5 times 10^8 cells/point). Immunoprecipitates were resolved by SDS-PAGE and then analyzed by anti-phosphotyrosine immunoblotting. The migration positions of the tyrosine-phosphorylated p145, Syk, and Shc proteins are indicated.



Phosphorylation of p145 and Shc in Response to IL-4

Next, we wanted to see if other means of stimulating B cells would also lead to the signaling events described above. We chose to examine IL-4, as this cytokine has several physiologically important effects on B lymphocytes. For example, IL-4 can increase surface expression of class II major histocompatibility complex proteins, induce class switching to IgE, and potentiate proliferation induced by BCR stimulation. The signaling mechanisms that lead to these responses are only partly understood. It is known that, as with other cytokine/hematopoeitin receptors, the IL-4 receptor can activate JAK kinases and STAT transcription factors(32) . In addition to JAK3 (33, 34, 35) and JAK1(36) , the IL-4 receptor has been reported to activate the intracellular tyrosine kinase, Fes(37) . In contrast, evidence is lacking for involvement of Src family tyrosine kinase or of Syk tyrosine kinase in IL-4 signaling in B lymphocytes. To examined the effects of IL-4 on Shc, Syk, and p145 tyrosine phosphorylation and association in B lymphocytes, we treated WEHI-231 B cells or splenic B cells with IL-4 and then examined tyrosine phosphorylation of immunoprecipitated proteins by anti-phosphotyrosine immunoblotting. IL-4 treatment resulted in clear tyrosine phosphorylation of Shc and an increase in Shc-associated tyrosine-phosphorylated p145 ( Fig. 6and data not shown). This response was less dramatic than that seen in response to anti-IgM but was still readily detectable. In contrast, the tyrosine phosphorylation of Syk was not induced by IL-4 stimulation and tyrosine-phosphorylated p145 protein was not coimmunoprecipitated with Syk (data not shown). As Syk activation is correlated with increased tyrosine phosphorylation of Syk, it is likely that Syk was not activated in B cells responding to IL-4. Thus, the association of tyrosine-phosphorylated p145 with Syk was dependent on the nature of the stimulation and did not necessarily correlate with the tyrosine phosphorylation of p145. This observation suggests first, that Syk may require tyrosine phosphorylation and/or activation to associate with p145 and second, that tyrosine kinases other than Syk can phosphorylate p145 and Shc under certain physiological conditions.


Figure 6: IL-4 receptor-induced tyrosine phosphorylation of Shc and p145. WEHI-231 cells were stimulated with medium(-), anti-IgM at 25 µg/ml for 3 min (BCR), or IL-4 at 100 units/ml for 5 or 10 min. Lysis, immunoprecipitation, and immunoblotting were performed as in Fig. 1. The position of the Shc and p145 proteins are indicated. Longer exposures revealed a p145 doublet induced by IL-4, although only one band of the doublet is visible in the data shown. IL-4 responses were maximum at 100 units/ml IL-4. Tyrosine phosphorylation induced by IL-4 was roughly equivalent to that induced through the antigen receptor using 1-3 µg/ml anti-IgM.



Phosphorylated Shcbulletp145 and Sykbulletp145 Complexes in Macrophages

To see if the association of p145 with Shc and with Syk occurs in response to stimulation of other receptors, we examined tyrosine phosphorylation of p145, Shc, and Syk in macrophages stimulated through Fc receptors. Like the BCR and the TCR, the FcR complexes have cytoplasmic domains with immunoreceptor tyrosine-based activation motif (ITAM) sequences (38) and therefore activate signal transduction events by a very similar mechanism. Cross-linking the FcRs in the murine macrophage cell line RAW 264.7 by addition of the anti-FcR antibody 2.4G2 and of a secondary cross-linking antibody resulted in tyrosine phosphorylation of Shc and the association of Shc with tyrosine-phosphorylated p145 (Fig. 7). Cross-linking the FcRs in these macrophages also induced tyrosine phosphorylation of Syk and the appearance of a low level of tyrosine-phosphorylated p145 associated with Syk, which was easily visible upon longer exposure of immunoblots (Fig. 7B). The low levels of Syk tyrosine phosphorylation and Shcbulletp145 complex formation that were observed in cells not receiving cross-linking treatment were equivalent to those seen in untreated cells.


Figure 7: A 145-kDa tyrosine-phosphorylated protein coimmunoprecipitates with Shc and Syk in macrophages. RAW 264.7 cells (5 times 10^6 cells/ml) were exposed to LPS (5 µg/ml) for 5 min at 37 °C. Alternatively, cells stimulated through the FcR were incubated on ice for 30 min (10 times 10^6 cells/ml) with purified monoclonal antibody (2.4G2) against murine FcR (40 µg/ml). The latter cells were warmed to 37 °C and exposed to a cross-linking mouse anti-rat Ig antibody (10 µg/ml). Anti-Shc and anti-Syk immunoprecipitated complexes were analyzed by SDS-PAGE and anti-phosphotyrosine immunoblotting. In panel A, Shc and Syk complexes were isolated from 3 mg of untreated, LPS, or cross-linked-FcR-stimulated cells. The positions of Syk, Shc, and the p145 protein are indicated. In a separate experiment (B), Shc and Syk proteins were immunoprecipitated from lysates of FcR-stimulated cells. As in A, cells were incubated with anti-FcR antibody on ice. After warming to 37 °C, half the cells were exposed to cross-linking antibody (``+'') and half were not (``-'').



Another potent stimulator of macrophages is bacterial LPS. LPS treatment of macrophages induces them to produce numerous cytokines and proinflammatory mediators(39, 40) . LPS binding to the glycosylphosphatidylinositol-linked protein CD14 leads to increased tyrosine phosphorylation in macrophages, and this event is important for downstream responses(41, 42, 43) . Although CD14 lacks any cytoplasmic tail sequences for direct association and activation of cytoplasmic protein kinases or signaling proteins, LPS rapidly activates the Src family tyrosine kinases Lyn, Hck, and Fgr in human monocytes suggesting an important role for these tyrosine kinases in LPS responses(44, 45) . Interestingly, RAW 264.7 cells stimulated with LPS exhibited induced tyrosine phosphorylation of Shc and Shc-associated p145 (Fig. 7A). The induced tyrosine phosphorylation and association of Shc and p145 was greater than that which occurred in response to FcR cross-linking. Moreover, these were rapid events in macrophages, being evident as early as 1 min and peaking at about 10 min after stimulation (data not shown). Unexpectedly, LPS signaling also induced tyrosine phosphorylation of Syk and its association with p145 (Fig. 7A). As was true for p145bulletShc association, the p145bulletSyk association (as detected by anti-phosphotyrosine immunoblotting) was greater in response to LPS than in response to FcR cross-linking. This is most likely due to the relatively low level of FcR expression on these cells. Cross-linking of higher levels of FcR expressed on other macrophage lines elicited a signal equivalent to or greater than that induced by LPS. Syk and Shc did not appear to associate in a precipitable complex from FcR- or LPS- stimulated macrophages, similar to the situation with BCR-stimulated B cells (data not shown). Although it has been reported that Syk becomes tyrosine-phosphorylated in response to FcR cross-linking and is important in phagocytosis(46, 47) , a role for Syk in LPS receptor signaling in macrophages had not been previously suggested. These observations were particularly interesting, as few downstream targets of tyrosine kinases in LPS-activated macrophages have been identified. Aside from the observations regarding Syk and Shc reported here, the only other reported early substrate for tyrosine kinases activated by LPS is the proto-oncogene product Vav(48) . Further characterization of the role of Shc and Syk in LPS-induced signaling in macrophages is currently under way and will be the subject of another report.


DISCUSSION

In agreement with previous reports(4, 49) , we have found that BCR stimulation of B lymphocytes leads to tyrosine phosphorylation of Shc and appearance of a tyrosine-phosphorylated protein doublet of apparent molecular weight 145,000 in Shc immunoprecipitates. We have additionally found that this association between Shc and p145 was induced upon BCR signaling. The quantity of Shcbulletp145 complexes in BCR-stimulated B cells decreased coincidently with the amount of tyrosine phosphorylation of the immunoprecipitated Shc and p145. These observations suggest that tyrosine phosphorylation of p145 and/or Shc was important for complex formation and maintenance. Kavanaugh et al.(13) found that recombinant Shc protein expressed in insect cells could associate with phosphorylated but not with dephosphorylated p145 immobilized on nitrocellulose membranes. Taken together with these results, our findings suggest that BCR engagement induced the tyrosine phosphorylation of p145 and this in turn led to Shc binding.

The mechanisms by which activation of the BCR-induced tyrosine phosphorylation of Shc and p145 are not known. One possibility is that intracellular tyrosine kinases activated by the BCR interacted directly with these signaling components. For example, Lyn and Fyn have been shown to associate with phosphatidylinositol 3-kinase in some situations where the latter component becomes activated(50, 51) . In addition to Src family tyrosine kinases, Syk and the highly related ZAP-70 tyrosine kinases play critical roles in signaling by antigen receptors(52) , and by FcRI and FcR(46, 53) . Upon immunoprecipitation of Lyn and Syk from BCR-stimulated B cells, we found tyrosine-phosphorylated p145 associated with Syk but not Lyn. In contrast, we did not detect any association of Shc with Syk. Recently, an interaction between Syk and Shc was detected following overexpression of Syk in B cells(54) . It could be that a very small amount of ShcbulletSyk complex did form in vivo in our BCR-stimulated B cells but was below our limit of detection. Such a small amount of Shc would not be sufficient to account for the prominent association of p145 with Syk, however, so p145bulletSyk association must either be direct or mediated via an association of both Syk and p145 with a protein or proteins other than Shc. In any case, the association of p145 with Syk is provocative and may reflect a role for Syk in phosphorylating p145 and possibly Shc as well.

The Syk-associated 145-kDa doublet appeared to be the same as the Shc-associated p145 doublet by several criteria. First, the distinctive pattern of tyrosine phosphorylation of the Shc-associated p145 was identical to that produced by the Syk-associated p145. In murine cells, the upper band was a more highly phosphorylated and more abundant protein than the lower band. In chicken B cells, the banding pattern was different from that found in mammalian B cells, yet the Syk-associated p145 again resembled the Shc-associated p145. Second, the association of p145 with Syk or Shc occurred with similar kinetics (data not shown). Third, murine GST-Shc protein was able to bind Shc-associated p145 or Syk-associated p145 immunoprecipitated from both murine and chicken cells and immobilized on nitrocellulose. And finally, a monoclonal antibody specific for Shc-associated p145 was reactive with Syk-associated p145 protein as well. Thus, the Syk-associated p145 and the Shc-associated p145 behaved identically in many respects, strongly suggesting their identity.

The tyrosine phosphorylation of Shc and the association of Shc with tyrosine-phosphorylated p145 were also seen in B cells stimulated through the IL-4 receptor, a member of the cytokine/hematopoietic receptor superfamily, and in macrophages stimulated either through FcRs or by LPS. We observed that FcR cross-linking or LPS stimulation of macrophages also induced tyrosine phosphorylation of Syk and its association with p145. IL-4 stimulation of B cells did not lead to Syk phosphorylation or its association with tyrosine-phosphorylated p145. Thus, association of p145 with Syk correlated with the tyrosine phosphorylation of Syk and not with the tyrosine phosphorylation of p145. While the mechanism by which p145 and Syk associate with each other remains to be determined, these results suggest that Syk may not associate via its SH2 domains with p145 or that the SH2 domains of Syk are inaccessible when the kinase is inactive. In support of the first suggestion, we have not detected an interaction between a GST-Syk(SH2)(2) fusion protein and p145 immobilized on membranes. (^3)

The Sykbulletp145 association observed in response to BCR-, FcR- and LPS-mediated activation suggests a role for Syk in phosphorylating either p145 or Shc. If that is the case then the IL-4 receptor presumably utilizes a different mechanism for phosphorylating p145 and Shc. Additionally, our results comparing BCR cross-linking and IL-4 receptor signaling in B cells indicated that different p145 associations were induced by kinases activated through different receptors within the same cell. This result raises the possibility that phosphorylated p145, although it is an early substrate of tyrosine kinases, may function in separate and distinct signaling pathways depending on the nature of the signaling receptor.

The functional significance of the p145bulletShc and p145bulletSyk complexes are not yet known, although several possibilities can be considered. These complexes appeared to be primarily membrane-bound in BCR-stimulated B cells. (^4)Association with p145 might concentrate Shc in a particular location in the cell, such as the plasma membrane. Thus, p145 could function to bring Shc to the membrane where its subsequent tyrosine phosphorylation and association with Grb-2bulletSOS-1 complex could promote Ras activation. In agreement with this idea, Saxton et al.(4) have shown that Shcbulletp145 complexes are present in cytosolic and membrane fractions of cells and that BCR stimulation resulted in elevated levels of Shc complexes in the membrane-bound fraction. It is unclear whether p145 or some other component is responsible for this membrane localization. Moreover, studies in other cell types have given different results regarding the molecular nature of the interactions between Shc, p145, and Grb-2, the intracellular localization of these complexes, and whether these proteins are within a single complex(4, 11, 21, 22) . It is unclear at this time whether these differences are due to the different experimental approaches utilized or, as reflected in our own results regarding Sykbulletp145 and Shcbulletp145 complexes, whether the exact nature of the complex may vary with the cell type and route of stimulation.

It is possible that p145 membrane localization is due to its association with tyrosine-phosphorylated receptor cytoplasmic domains, as has been reported for Syk. In this model, the coprecipitation of Syk and p145 would reflect a trimolecular complex consisting of phosphorylated receptor tails interacting with both p145 and Syk. Interestingly, it has recently been reported that stimulation of the FcRI on mast cells resulted in coimmunoprecipitation of a tyrosine-phosphorylated protein of 145 kDa with the FcRI beta chain (53) . In this system, a GST-Syk(SH2)(2) fusion protein was used to precipitate not only the and beta chains of the FcRI, but also a tyrosine-phosphorylated doublet resembling p145 (53, 55) . These observations may reflect associations of GST-Syk(SH2)(2) and p145 with the FcRI chain and beta chain cytoplasmic domains. Interestingly, we have observed a small amount of p145 protein to coprecipitate with Ig-alpha from lysates of BCR-stimulated B cells.^3 Thus, one possible scenario is that BCR and FcR stimulation leads to p145 association with tyrosine-phosphorylated receptor tails, followed by tyrosine phosphorylation of p145, dissociation of Syk, and subsequent association of Shc with p145. This could be a means whereby Shc would achieve recruitment to the membrane and subsequent phosphorylation on Tyr-317. Phosphorylated Shc could then be bound by the Grb-2(SH2) domain of a Grb-2bulletSOS-1 complex resulting in activation of Ras at the membrane. The exact nature of the interactions between Syk, ITAM-containing receptor chains and p145 and whether this association is used as a mechanism for Ras activation remain to be determined.

The observations reported here suggest that p145 may be an important signaling component. The membrane localization of p145 and its association with Shc in B cells stimulated through the BCR are consistent with p145 playing an important role in Ras activation. It is equally possible that p145 is a signaling effector that is regulated by Shc and therefore represents a non-Ras Shc-signaling pathway. Two of the receptors that induced Shc tyrosine phosphorylation and association with p145, IL-4R in B cells and FcR in macrophages, have not been reported to result in Ras activation. LPS may activate Ras in monocytes and macrophages, although this has not been uniformly observed to date (56, 57) . Thus the downstream consequences of Shc and p145 tyrosine phosphorylation in B cells or in macrophages remains unknown. The cloning of the gene for p145 and subsequent characterization of its primary sequence may shed light on these issues. In any case, the ability of p145 to interact with the cytoplasmic signaling components Shc and Syk suggests that it plays an important role in the initiation of signaling events in activated B cells and macrophages.


FOOTNOTES

*
This work was supported in part by Grants AI20038 and AI33442 from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
The first two authors contributed equally to the experiments in this paper.

Supported by National Institutes of Health Training Grant T32AI07334.

**
Howard Hughes Medical Institute predoctoral fellow.

§§
To whom correspondence should be addressed: George W. Hooper Foundation, University of California, San Francisco, CA 94143-0552.

(^1)
The abbreviations used are: BCR, B cell antigen receptor; IL, interleukin; LPS, lipopolysaccharide; SH2, Src homology 2; GST, glutathione S-transferase; GST-Syk(SH2)(2), fusion protein consisting of both SH2 domains of Syk fused to GST; PAGE, polyacrylamide gel electrophoresis; TCR, T cell receptor; FcR, Fc receptor for IgG; FcR, Fc receptor for IgE; HRP, horseradish peroxidase; PBS, phosphate-buffered saline; ITAM, immunoreceptor tyrosine-based activation motif.

(^2)
J. D. Richards, M. R. Gold, S. L. Hourihane, A. L. DeFranco, and L. Matsuuchi, submitted for publication.

(^4)
M. T. Crowley and A. L. DeFranco, unpublished results.

(^3)
S. L. Harmer and A. L. DeFranco, unpublished observations.


ACKNOWLEDGEMENTS

We thank Dr. Tomohiro Kurosaki for the generous gift of the chicken B cell lines DT40 and S1.10. We thank Drs. Khoi Lee and Lewis Williams for the plasmid pG-IKS. We thank Dr. P. G. Pelicci for the Shc cDNA clone. We thank Steve Robbins, Steve Weinstein, Julie Hambleton, Debbie Law, and Jim Richards for many helpful discussions and for critical reading of the manuscript.


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