©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
Function of the htrB High Temperature Requirement Gene of Escherichia coli in the Acylation of Lipid A
HtrB CATALYZED INCORPORATION OF LAURATE (*)

(Received for publication, February 8, 1996)

Tony Clementz Jeffrey J. Bednarski Christian R. H. Raetz (§)

From the Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

By assaying lysates of Escherichia coli generated with the hybrid bacteriophages of an ordered library (Kohara, Y., Akiyama, K., and Isono, K.(1987) Cell 50, 495-508), we identified two clones (232 and 233) capable of overexpressing the lauroyl transferase that functions after 3-deoxy-D-manno-octulosonic acid (Kdo) addition in lipid A biosynthesis (Brozek, K. A., and Raetz, C. R. H.(1990) J. Biol. Chem. 265, 15410-15417). The E. coli DNA inserts in 232 and 233 suggested that a known gene (htrB) required for rapid growth above 33 °C might encode the lauroyl transferase. Using the intermediate (Kdo)(2)-lipid IV(A) as the laurate acceptor, extracts of strains with transposon insertions in htrB were found to contain no lauroyl transferase activity. Cells harboring hybrid htrB plasmids overproduced transferase activity 100-200-fold. The overproduced transferase was solubilized with a non-ionic detergent and purified further by DEAE-Sepharose chromatography. With lauroyl acyl carrier protein as the donor, the purified enzyme rapidly incorporated one laurate residue into (Kdo)(2)-lipid IV(A). The rate of laurate incorporation was reduced by several orders of magnitude when either one or both Kdos were absent in the acceptor. With a matched set of acyl-acyl carrier proteins, the enzyme incorporated laurate 3-8 times faster than decanoate or myristate, respectively. Transfer of palmitate, palmitoleate, or R-3-hydroxymyristate was very slow. Taken together with previous studies, our findings indicate that htrB encodes a key, late functioning acyltransferase of lipid A biosynthesis.


INTRODUCTION

Lipid A, the hydrophobic anchor of the outer membrane lipopolysaccharide of Escherichia coli(1, 2, 3, 4, 5) , consists of a glucosamine disaccharide that is phosphorylated at positions 1 and 4`, and is acylated with R-3-hydroxymyristate at positions 2, 3, 2`, and 3` (Fig. 1). Lipid A of wild-type E. coli cells contains two additional fatty acyl chains, primarily laurate and myristate(3, 6, 7, 8) . The latter are esterified to the R-3-hydroxy groups of the distal glucosamine residue (Fig. 1), forming the acyloxyacyl moieties that are characteristic of lipid A (1, 3, 6, 7, 8) . Variations in the composition and location of the non-hydroxylated acyl chains can occur(1, 8) . For instance, minor lipid A species are observed in E. coli in which the myristate residue is missing entirely (8) or is replaced with laurate (not shown in Fig. 1)(9) . An additional palmitate residue may also sometimes be present in acyloxyacyl linkage on the proximal glucosamine unit (not shown in Fig. 1), either with or without the myristate on the distal glucosamine(1, 4, 8) . The laurate residue that is attached to the distal N-linked R-3-hydroxymyristate (Fig. 1) is found in almost every lipid A moiety of wildtype cells (8, 9) .


Figure 1: Pathway for the late stages of lipid A biosynthesis in E. coli. Evidence for the complete scheme has recently been reviewed(2, 3, 5) .



In previous studies, we described the existence of novel acyltransferase(s) in extracts of E. coli that can incorporate one or two laurate (or myristate) residues into lipid A precursors(10) . The lauroyl/myristoyl transferase(s) require lauroyl- or myristoyl acyl carrier proteins (ACP) (^1)as donors(10) . They appear to function at a late stage of lipid A assembly, since they recognize lipid A disaccharide precursors that are glycosylated with Kdo as substrates (Fig. 1)(10) . Their extraordinary Kdo dependence explains why lipid IV(A) (Fig. 1), rather than fully acylated lipid A, accumulates in living cells when the biosynthesis of Kdo is interrupted(11, 12, 13, 14) .

Prior to the present work, no late acyltransferase had been purified (10) , and gene(s) encoding these enzyme(s) were not known(3) . The issue of whether one or more distinct acyltransferases were required for acyloxyacyl group formation was not resolved(10) . A priori, it seemed reasonable that a separate enzyme would be needed for the generation of each acyloxyacyl moiety. In cell extracts, two laurates, two myristates, or one of each can be transferred efficiently to (Kdo)(2)-lipid IV(A)(10) , despite the distinct composition of the dominant lipid A molecular species made in wild-type cells (Fig. 1)(7, 8) .

To identify a gene encoding a Kdo-dependent late acyltransferase, we have assayed individual E. coli lysates generated with each of the hybrid bacteriophages of the Kohara library (15, 16) for overproduction of the lauroyl transferase that acts on (Kdo)(2)-lipid IV(A) (Fig. 1)(10) . In this manner, we have discovered that a known gene (htrB), described by Karow and Georgopoulos (17, 18, 19, 20, 21) as required for growth on rich media above 33 °C, encodes the lauroyl transferase (Fig. 1). This finding explains why lipid A isolated from htrB deficient mutants contains very little laurate(20) . The msbB gene(19) , a high multi-copy suppressor of the temperature sensitivity associated with htrB mutations, encodes a separate late acyltransferase with a strong kinetic preference for the penta-acylated lipid generated (Fig. 1) by HtrB (22) . The envelope-related alterations associated with mutations in the htrB gene(17, 19) , such as bulging of the cell surface and deoxycholate resistance, can now be explored in a biochemical context. A preliminary communication of our results has appeared in abstract form(22) .


EXPERIMENTAL PROCEDURES

Materials

[-P]ATP was obtained from DuPont NEN. Pyridine, chloroform, methanol, and 88% formic acid were from Fisher. All detergents were of high quality grade (peroxide and carbonyl free). Triton X-100 was Surfact-Amps grade from Pierce, and Thesit was from Boehringer Mannheim. Acyl carrier protein was purchased from Sigma. Other items were obtained from the following companies: 0.25-mm glass-backed Silica Gel 60 thin-layer chromatography plates, E. Merck; yeast extract and Tryptone, Difco; and DEAE-Sepharose CL-6B, Pharmacia.

Bacterial Strains and Plasmids

Strains used in this study are derivatives of E. coli K12, and their genotypes are listed in Table 1. Cultures were grown in Luria broth, consisting of 5 g of NaCl, 5 g of yeast extract, and 10 g of Tryptone/liter(23) . Antibiotics were added, when required, at 100 µg/ml for ampicillin, 12 µg/ml for tetracycline, and 10 µg/ml for chloramphenicol.



Recombinant DNA Techniques

Plasmid DNAs were isolated using the Wizard miniprep kit (Promega). Other recombinant DNA techniques were performed as described previously(24) .

Screening of the Kohara Library for Overproduction of Lauroyl Transferase Activity

A set of fresh lysates was created from a copy of the original Kohara library(15, 16) . A culture of the host strain, E. coli W3110, grown to saturation overnight in LB broth supplemented with 10 mM MgSO(4) and 0.2% maltose, was mixed with an equal volume of 10 mM MgCl(2) and 10 mM CaSO(4). Using several 96-well microtiter plates, 10-µl portions of the host cell suspension were mixed with 5 µl of each of the 476 lysates of the Kohara library(15, 16) . After allowing 15 min for infection at 37 °C, 150-µl portions of LB broth supplemented with 10 mM MgSO(4) were added to each well. The lysates from the original library were used undiluted or diluted 1/10, as required, to accomplish lysis of the W3110 host within 5-8 h after infection. The microtiter plates were incubated on a shaker at 37 °C. At 4.5 h after infection, and at 30-min intervals thereafter, the plates were inspected for wells displaying complete lysis. As soon as cultures showed lysis, the lysates were transferred to a new microtiter plate and stored at 4 °C, while the unlysed cultures in the original microtiter plate were incubated further at 37 °C. At the end of the experiment, the lysates were all stored at -80 °C.

For the purpose of screening the entire library, lysates (0.5 µl of a 1:5 dilution) were assayed for lauroyl transferase activity in a 10-µl screening assay mixture, containing 50 mM Hepes pH 7.5, 0.1% Triton X-100, 10 µM (Kdo)(2)-[4`-P]lipid IV(A) (1 times 10^4 dpm/nmol), 25 µM lauroyl-ACP, and 0.2 mg/ml bovine serum albumin. Reactions were incubated for 10 min at 30 °C. A 5-µl portion of each reaction mixture was spotted onto a Silica Gel 60 thin layer chromatography plate, which was developed and subjected to PhosphorImager analysis to determine the extent of acylation catalyzed by each extract (see below).

Assay for Lauroyl-ACP-dependent Acylation of (Kdo)(2)-lipid IV(A)

To determine the specific activity of the lauroyl transferase in various extracts and purified fractions, assays were generally performed at 30 °C, as described previously(10) . The standard reaction mixture (Method I) contained 50 mM Hepes pH 7.5, 0.1% Triton X-100, 25 µM (Kdo)(2)-lipid IV(A) (2 times 10^3 dpm/nmol), 25 µM lauroyl-ACP, O.1 mg/ml bovine serum albumin, and 0.4-1000 µg/ml enzyme protein, as indicated, in a final volume of 20 µl. Reactions were stopped by spotting a 4-5-µl portion onto a Silica Gel 60 thin layer chromatography plate. After a few minutes to allow the spots to dry, the plates were developed in chloroform, pyridine, 88% formic acid, water (30:70:16:10, v/v), and then exposed to a PhosphorImager screen. The amounts of radioactivity in both the substrate and the acylated product were measured using a Molecular Dynamics PhosphorImager. The specific activity of the lauroyl transferase was expressed in terms of nanomoles/min/mg of protein.

In the course of purifying the lauroyl transferase, an improved assay was developed (Method II). The latter was identical to Method I, except that 5 mM MgCl(2) and 50 mM NaCl were included in the reaction mixture to stabilize the enzyme.

Preparation of Cell-free Extracts, Membranes, and Soluble Fractions for Analysis of the Lauroyl Transferase

Crude cell-free extracts were made from 1-2 liters of logarithmically growing cultures. After harvesting by low speed centrifugation at 2 °C, cells were washed once in 30 mM Hepes pH 7.5 containing 1 mM EDTA and 1 mM EGTA (half the volume of the original culture). The washed cell pellet was resuspended in 30 mM Hepes pH 7.5 containing 1 mM EDTA and 1 mM EGTA (a volume approximately equal to the volume of the cell pellet). Cells were broken using an ice-cold French Pressure cell (SLM Instruments, Urbana, IL) at 20,000 p.s.i. The broken cell suspension was adjusted to 10 mM MgSO(4), and DNase I was added to 1 µg/ml. After a brief sonication on an ice water bath to decrease the viscosity, the suspension was incubated for 30 min at 30 °C. Unbroken cells were removed by centrifugation at 1000 times g for 10 min. Membranes and soluble fractions were separated by centrifugation at 150,000 times g for 60 min. The supernatant was centrifuged a second time to remove residual contaminating membranes. The membrane pellet was resuspended in 25 ml of 30 mM Hepes pH 7.5 containing 1 mM EDTA and 1 mM EGTA, and it was centrifuged again as above to generate the final, washed membrane fraction.

Protein concentrations were determined with the bicinchoninic assay (Pierce), using bovine serum albumin as the standard(25) .

Isolation and Preparation of Substrates

Lipid IV(A)(13) , (Kdo)(2)-lipid IV(A)(26, 27) , [4`-P]lipid IV(A)(28, 29) , and (Kdo)(2)-[4`-P]lipid IV(A)(26, 27) were prepared as described previously. A derivative of [4`-P]lipid IV(A) bearing only one Kdo residue (designated Kdo-[4`-P]lipid IV(A)) was synthesized from [4`-P]lipid IV(A) under the same conditions used to make (Kdo)(2)-[4`-P]lipid IV(A), except that membranes (1 mg/ml final concentration) isolated from Haemophilus influenzae(30) were substituted for the purified E. coli Kdo transferase(27) .

Various acyl-ACPs were synthesized from the corresponding fatty acids and commercial acyl carrier protein, as described previously(31) , except that the immobilized acyl-ACP synthase was replaced with 80 µg/ml solubilized membrane protein from the acyl-ACP synthase overproducing strain, E. coli LCH109pLCH5/pGP1-2(32) .

To obtain the acyl-ACP synthetase, LCH109/pLCH5/pGP1-2 membranes were solubilized using a modification of the procedure of Rock and Cronan (32, 33) . Two 1-liter cultures of LCH109/pLCH5/pGP1-2 were grown at 30 °C until the cultures reached an A of approximately 0.4. The cultures were then shifted to 42 °C for 30 min. Next, the cultures were allowed to continue growing for 90 min at 37 °C. To harvest, the cells were centrifuged at 4500 times g for 10 min and resuspended in 15 ml of 50 mM Tris-HCl, pH 8.0. A French pressure cell at 18,000 p.s.i. was used to disrupt the cells, and unbroken cells were removed by centrifuging at 1000 times g for 10 min. The supernatant was made 10 mM in MgCl(2) by adding 1 M MgCl(2). Then, the extract was centrifuged for 1 h at 150,000 times g. The pellet was resuspended in 5 ml of 50 mM Tris-HCl, pH 8.0. Next, a 5-ml solution of 50 mM Tris-HCl, pH 8.0, containing 4% Triton X-100 and 20 mM MgCl(2) was added to solubilize the inner membrane proteins. The suspension was stirred on ice for 30 min. The solubilized membranes were centrifuged a second time at 150,000 times g for 1 h to remove outer membrane proteins, which were not extracted efficiently under these conditions. The supernatant was stored in aliquots at -80 °C.

The enzymatic acylation of ACP with laurate, myristate, palmitate, decanoate, palmitoleate, or R-3-hydroxymyristate was carried out as follows. ACP (1 mg) and 8.6 mM dithiothreitol were incubated in 500 µl of 40 mM Tris-HCl, pH 8.0, in a sealed tube at 37 °C for 1 h. Next, a 320-µl solution consisting of 0.7 M LiCl, 40 mM MgCl(2), 20 mM ATP, pH 8.0, 750 µM fatty acid, 2.7% Triton X-100, and 540 mM Tris-HCl, pH 8.0, was added to the tube with the ACP. Last, 400 µl of 0.25 mg/ml LCH109/pLCH5/pGP1-2 solubilized membranes was added, and the acylation reaction was allowed to proceed at room temperature for 1-2 h. The extent of acylation was determined by analyzing 5-µl portions of the reaction mixture on a polyacrylamide/urea gel system(34) .

To isolate the product, the reaction mixture was diluted 10-fold with water and loaded onto a 1-ml column of DEAE-Sepharose equilibrated with 10 mM bis-tris, pH 6.0. The column was washed with 5 bed volumes of 10 mM bis-tris, pH 6.0, 5 volumes of 10 mM bis-tris, pH 6.0, containing 50% isopropyl alcohol, and 5 volumes of 10 mM bis-tris, pH 6.0. The column was eluted with 3 volumes of 10 mM bis-tris, pH 6.0, containing 0.2 M LiCl and 3 volumes of 10 mM bis-tris, pH 6.0, containing 0.6 M LiCl. Fractions of 1 ml were collected. The acyl-ACPs eluted in the second 0.6 M LiCl fraction. This fraction was concentrated and exchanged into distilled H(2)O using a Centricon-3 membrane (Amicon). The acyl-ACPs were about 90% pure, as judged by electrophoresis in the polyacrylamide/urea gel system and staining with Coomassie Blue (34) .


RESULTS

Preparation of Kohara Library Lysates on E. coli W3110

Lysates were generated in E. coli strain W3110, using an ordered subset of the E. coli chromosomal library of Kohara(15, 16) , as described under ``Experimental Procedures.'' Every insert in this ``miniset'' collection has been mapped with restriction enzymes and positioned on the E. coli chromosome(15, 16) , facilitating the identification of genes. Satisfactory lysates were obtained with 461 of the 476 clones that constitute the miniset collection. The remaining 15 clones did not cause lysis of E. coli W3110, probably because of a low titer in the original library lysates. No attempt was made to retrieve these 15 clones.

Assaying Enzymes of Lipid Biosynthesis in the Lysates

Before screening all the lysates for overproduction of the lauroyl transferase, the ability of these lysates to overexpress a characterized enzyme of lipid A biosynthesis with a known structural gene was validated. The kdtA gene, encoding Kdo transferase (Fig. 1), was previously mapped to the overlapping DNA segments present in clones [573]4D2 and [572]2A6 of the Kohara library(16, 35) . The lysates generated with these clones on W3110 displayed a 5-10-fold higher specific activity of Kdo transferase than lysates generated from other, randomly selected clones (data not shown). Overexpression, like that seen with kdtA, was also observed in lysates generated with clones harboring two other mapped genes of lipid biosynthesis (dgk and glpK)(16) . These findings demonstrate that certain cloned E. coli genes present on non-lysogenic hybrid vectors are transcribed and translated efficiently during infection. It is possible to detect clones containing a gene of interest by assaying for overexpression of enzymatic activity above the background derived from the chromosomally encoded gene. Screening for overexpression of enzyme activity in lysates prepared with hybrid libraries therefore affords a useful alternative for identifying novel genes in such libraries, especially if no sequence information is available.

Screening for E. coli DNA Fragments Encoding the Lauroyl Transferase

Given the results with the Kdo transferase, all 461 lysates generated in E. coli strain W3110 with the Kohara library were assayed for overproduction of the lauroyl transferase that acts on (Kdo)(2)-[4`-P]lipid IV(A) (Fig. 1)(10) . Appropriate dilutions of these lysates were used to give a conversion within the linear range of the assay (1-10% under these conditions) for the chromosomally encoded activity, as described under ``Experimental Procedures.'' Screening the lysates in this manner resulted in the identification of two clones (Fig. 2) that overexpressed the lauroyl transferase, as judged by thin layer chromatography and PhosphorImager analysis. Lysates from clones [232]1H7 and [233]E4H10S, containing adjacent and partially overlapping chromosomal fragments, catalyzed 25 and 45% conversion of substrate to product, respectively, as compared to 5.5 and 7.1% for the two flanking clones, [231]1C7 and [234]E3G11 (Fig. 2). The overexpressed transferase displayed an absolute dependence on the addition of lauroyl-ACP as a cosubstrate (Fig. 2), as did the transferase present in extracts of control cells.


Figure 2: Detection of clones in the Kohara library that direct overexpression of lauroyl transfer to (Kdo)(2)-[4`-P]lipid IV(A). The hybrid bacteriophages of the Kohara library were used to infect and generate new lysates on E. coli W3110. Following their identification with the initial screening assay (see ``Experimental Procedures''), selected lysates (2 µl of a 1:10 dilution) were re-assayed to confirm overproduction of lauroyl transferase activity in a 20-µl assay mixture, containing 50 mM Hepes pH 7.5, 0.1% Triton X-100, 10 µM (Kdo)(2)-[4`-P]lipid IV(A) (1 times 10^4 dpm/nmol), 25 µM lauroyl-ACP, and 0.2 mg/ml bovine serum albumin. After 10 min at 30 °C, the reactions were stopped by spotting 5 µl onto Silica Gel 60 thin-layer chromatography plates. The plates were developed in chloroform, pyridine, 88% formic acid, water (30:70:16:10, v/v), and radioactive spots were detected and analyzed with a PhosphorImager. Arrows indicate the positions of the substrate (Kdo)(2)-[4`-P]lipid IV(A) and the monoacylated product a after chromatography. The origin is at the bottom.



Genes Present on [232]1H7 and [233]E4H10S

The cloned DNA fragments in [232]1H7 and [233]E4H10S include approximately 11 kilobases of overlapping sequences, according to the EcoMap6(16) . Since both these clones directed overexpression of the transferase, we assumed that the entire gene encoding the transferase should reside within the overlap. A few sequenced genes had previously been mapped to this region (near minute 25), one of which is htrB(17, 18) . Strain MLK53, in which htrB is disrupted by Tn10 insertion (17, 19) , is unable to grow above 33 °C on rich media. MLK53 also is resistant to four times the concentration of deoxycholate as is wild-type E. coli, and addition of low levels of cationic detergents to the medium suppresses the temperature-sensitive growth associated with htrB mutations(19) . Since these properties, in conjunction with morphological alterations observed in MLK53(17, 19) , are indicative of changes in the outer membrane, htrB seemed to be a plausible candidate for a gene involved in lipid A biosynthesis. Although the htrB gene had been cloned and sequenced(17, 18) , no enzymatic function had been ascribed to its product.

The htrB Gene Encodes a Lauroyl Transferase Involved in Lipid A Biosynthesis

As shown in Fig. 3, crude cell free extracts prepared from E. coli MLK53(17, 19) , a strain in which the htrB gene is inactivated by insertion of Tn10, contained no detectable lauroyl transferase activity. Membranes of MLK53 also were devoid of lauroyl transferase (Table 2). Wild-type E. coli host strains transformed with the multi-copy plasmid pKS12(17) , harboring htrB, overexpressed the lauroyl transferase more than 100-fold, as judged by assaying crude extracts (not shown) or washed membranes (Table 2). The pKS12 expressed lauroyl transferase activity, like the wild-type, was located primarily in the membrane fraction. The observed membrane localization of the enzyme is consistent with the hydropathy profile of htrB(18, 19) . The large effects of htrB mutations or htrB overexpression on lauroyl transferase specific activity (Table 2) strongly suggest that htrB is the structural gene for the transferase.


Figure 3: Lauroyl transferase activity in extracts of E. coli W3110 compared to the htrB-deficient strain MLK53. Cell-free extracts (1.0 mg/ml) from strain MLK53 (lanes 1 and 2) and W3110 (lanes 3 and 4) were assayed in a reaction mixture containing 50 mM Hepes pH 7.5, 0.1% Triton X-100, and 25 µM (Kdo)(2)-[4`-P]lipid IV(A) (2 times 10^3 dpm/nmol). Lanes 1 and 3 also contained 25 µM lauroyl-ACP. Lanes 2 and 4 contained no added lauroyl-ACP. After incubation for 30 min at 37 °C, 5-µl portions of each reaction were spotted onto a Silica Gel 60 thin-layer chromatography plate, which was developed in CHCl(3), pyridine, 88% formic acid, water (30:70:16:10, v/v) and analyzed with a PhosphorImager (Molecular Dynamics), as in Fig. 2.





Karow and Georgopoulos (19) identified and isolated another gene of unknown function, designated msbB, that displays significant sequence homology to htrB. When present on hybrid plasmids maintained at very high copy numbers, msbB suppresses the temperature-sensitive growth phenotype of MLK53(19) . Unlike htrB, however, msbB is not essential for growth at any temperature(19) . Membranes of strain MLK1067(19) , harboring an insertion in msbB, contain normal amounts of lauroyl transferase (Table 2). Overexpression of msbB on hybrid plasmids does not result in increased levels of lauroyl transferase (data not shown). When pKS12 (htrB) is transformed into E. coli MLK1067 (msbB1::Cam)(19) , lauroyl transferase activity is overproduced, just as when pKS12 is introduced into wild-type strains, like XL1-Blue (Table 2). These findings are consistent with the hypothesis that htrB and msbB encode distinct enzymes, and are not likely to be regulatory genes affecting the same enzyme.

Myristoyl-ACP could replace lauroyl-ACP as the acyl donor in acylations catalyzed by crude extracts or isolated membranes from E. coli W3110, XL1-Blue/pKS12, or MLK1067/pKS12 (data not shown). However, the reaction rate with myristoyl-ACP as the donor and (Kdo)(2)-[4`-P]lipid IV(A) as the acceptor was 5-10-fold slower than with lauroyl-ACP. Extracts of MLK53 were unable to acylate (Kdo)(2)-[4`-P]lipid IV(A) when myristoyl-ACP was employed as the substrate (data not shown), as observed with lauroyl-ACP ( Fig. 3and Table 2).

Solubilization of Lauroyl Transferase from Membranes of Overproducing Strains and Fractionation on DEAE-Sepharose

The lauroyl transferase was solubilized in an active form from membranes of E. coli MLK1067/pKS12, using the Triton X-100 analog, Thesit. Membranes (3.8 ml at 18 mg/ml protein) were incubated for 2 h at 4 °C with an equal volume of solubilization buffer, yielding final concentrations of 2.5% Thesit, 20 mM Tris chloride, pH 7.8, 1 mM EDTA, 1 mM EGTA, 150 mM NaCl, 10% glycerol, and 100 mM potassium phosphate. Insoluble material was removed by centrifugation for 60 min at 170,000 times g. These conditions solubilized 98% of the lauroyl transferase activity, while only releasing about 30% of the total membrane proteins, giving a 3-fold purification before DEAE-fractionation (Table 3). The solubilized protein (20 mg) was diluted 4-fold with buffer consisting of 20 mM Tris chloride, pH 7.8, 1 mM EDTA, 1 mM EGTA, and 10% glycerol, and the sample was applied onto a 45-ml column of DEAE-Sepharose CL-6B, equilibrated with 20 mM Tris chloride, pH 7.8, 0.2% Thesit, 1 mM EDTA, 1 mM EGTA, 10% glycerol, and 50 mM NaCl. After washing with 90 ml of equilibration buffer, the bound proteins were eluted with a linear NaCl gradient in equilibration buffer from 50 to 500 mM NaCl (Fig. 4). Fractions containing the peak of HtrB activity (Fig. 4, gray area) were pooled and diluted 5-fold (as above) to lower the NaCl concentration. Proteins in the diluted peak fractions were then applied to a 1-ml column of DEAE-Sepharose, equilibrated as above, and eluted in a total volume of 1.8 ml of equilibration buffer containing 0.05% Triton X-100 and 250 mM NaCl. The final protein solution was stored in aliquots at -80 °C. Taken together, the plasmid encoded overexpression, solubilization, and DEAE-fractionation resulted in 1000-fold purification over wild-type membranes ( Table 2and Table 3). Although the lauroyl transferase was not purified to homogeneity, the solubilization and fractionation resulted in a highly concentrated enzyme preparation, allowing studies of its catalytic properties without interference from endogenous lipids.




Figure 4: Fractionation of the solubilized lauroyl transferase on DEAE-Sepharose CL-6B. Thesit solubilized membrane proteins (20 mg) were applied onto a 45-ml column as described in the text in detail. Proteins were eluted with a linear gradient from 50 to 500 mM NaCl. Absorbance at 280 nm (open circles) was used as a measure of total protein in each fraction. Lauroyl transferase activity (solid circles) was assayed with Method I using 5 µl of a 1:10 dilution of each fraction. Dotted line, calculated NaCl concentration. Gray area, active fractions that were pooled and concentrated.



Stability and Assay of the DEAE-Sepharose Purified Enzyme

After DEAE fractionation, assays of the lauroyl transferase with Method I indicated low and variable recovery of activity (less than 10%). Considering the presence of EDTA and EGTA during solubilization and fractionation, the effect of divalent cations was tested by including 1-5 mM MgCl(2) in the assay. Significant stimulation (as much as 50-fold with some substrate preparations) was observed after the DEAE step (data not shown). Addition of sodium chloride to the assay mixture in the absence of MgCl(2) also stimulated the activity considerably (as much as 10-fold).

Given these findings, the assay conditions were re-examined in more detail with the partially purified enzyme ( Fig. 5and 6). In the presence of 1 or 5 mM MgCl(2), the lauroyl transferase showed a dependence on added detergent that is typical of enzymes using lipid substrates (Fig. 5A). With Triton X-100, the highest activity was seen at concentrations between 0.1 and 0.2% (w/v). At higher Triton X-100 concentrations, the activity dropped off, presumably because of surface dilution of the substrate in mixed micelles. At 5 mM MgCl(2), the Triton X-100 optimum was considerably broader than at 1 mM (Fig. 5A), and therefore 5 mM MgCl(2) was included in all subsequent assays.


Figure 5: Effects of Triton X-100 and NaCl on lauroyl transferase activity and stability. Panel A, DEAE-Sepharose fractionated HtrB was assayed for 5 min at 30 °C at a protein concentration of 0.25 µg/ml under conditions similar to those described in the legend to Fig. 3, except that the concentration of Triton X-100 was varied, as indicated, and 1 mM MgCl(2) (solid circles) or 5 mM MgCl(2) (open circles) was also included. Panel B, DEAE-Sepharose purified HtrB (0.25 µg/ml) was preincubated without lauroyl-acyl carrier protein for 30 min at 30 °C under assay conditions similar to those of Fig. 3, but with 5 mM MgCl(2) included. Reactions were started by addition of 25 µM lauroyl-acyl carrier protein, and the reaction mixture was incubated at 30 °C for the times indicated. Open squares, preincubation with 50 mM NaCl in addition to the above components; open triangles, preincubation without added NaCl; open circles, no preincubation and no added NaCl.



Even in the presence of 5 mM MgCl(2), the DEAE-purified lauroyl transferase was unstable when preincubated under assay conditions (Fig. 5B, triangles). The addition of 50 mM NaCl together with 5 mM MgCl(2) to the reaction mixture was found to stabilize the enzyme to preincubation (Fig. 5B, open squares), resulting in a constant rate of acylation for 40-60 min at 30 °C and excellent linearity with respect to added protein (Fig. 6). All subsequent assays were therefore performed with both 5 mM MgCl(2) and 50 mM NaCl supplementation (assay Method II). The key assays of various membrane preparations and purified fractions shown in Table 2and Table 3were all performed using Method II. The effects of MgCl(2) and NaCl were less pronounced when crude extracts or membrane preparations were assayed (data not shown).


Figure 6: Dependence of lauroyl transfer on time and protein concentration under optimized conditions. DEAE-Sepharose purified HtrB was assayed at 30 °C using Method II (see ``Experimental Procedures'') with varying protein concentrations for 15 min (Panel A) or with varying incubation times at 0.2 µg/ml (Panel B).



Substrate Specificity of the Partially Purified Lauroyl Transferase

Lipid A isolated from E. coli K12 is primarily hexa-acylated (Fig. 1), but a minor penta-acylated species that lacks myristate is often observed(8) . This finding suggests that, at least in vivo, the addition of laurate occurs before the addition of myristate. In crude extracts, lauroyl-ACP is a more efficient acyl donor than myristoyl ACP (see above). To determine the acyl chain specificity of the purified lauroyl transferase, acyl-ACPs bearing chains of 10, 12, 14, and 16 carbons were tested as donors with (Kdo)(2)-[4`-P]lipid IV(A) as the acceptor. Using assay Method II, the relative rate of acylation was highest with lauroyl ACP (Fig. 7), as in crude extracts, although decanoyl-ACP and myristoyl-ACP supported acylation at about 30 and 12%, respectively, of the rate observed with lauroyl ACP. Other acyl-ACPs were not very effective substrates (Fig. 7). The fact that decanoate is not present in lipid A isolated from living cells (8) suggests that the acylation conditions in vivo are more selective. Perhaps, the concentration of decanoyl-ACP is much lower than that of lauroyl-ACP in cells.


Figure 7: Acyl chain length specificity of the lauroyl transferase. The relative initial rates of acylation of 25 µM (Kdo)(2)-[4`-P]lipid IV(A) were determined at 30 °C using different acyl-acyl carrier proteins as donors at 25 µM. Transferase activity was measured using Method II with 0.4 µg/ml of DEAE-Sepharose purified enzyme. The rates of acylation were normalized to that observed with 25 µM lauroyl-acyl carrier protein (12:0), which was defined as 1.0. The other acyl chains tested were: 10:0, decanoate; 14:0, myristate; HO14:0, R-3-hydroxymyristate; 16:0, palmitate; and 16:1c, palmitoleate.



The specificity of the purified enzyme for various acceptors was examined using assay Method II (Fig. 8). At 20 µg/ml of the DEAE-purified enzyme, complete conversion of 25 µM (Kdo)(2)-[4`-P]lipid IV(A) to the mono-acylated product (designated ``a'') was achieved in 30 min (Fig. 8, lane 3+). In contrast, less than 1% of [4`-P]lipid IV(A) or of Kdo-[4`-P]lipid IV(A) (both at 25 µM) was acylated under otherwise identical conditions (lanes 1+ and 2+, respectively). The results show that the purified lauroyl transferase functions at least 2 orders of magnitude more rapidly when both Kdo residues are present in the acceptor.


Figure 8: Lipid acceptor specificity of the htrB encoded acyl transferase. DEAE-Sepharose purified lauroyl transferase was used at 20 µg/ml (a large excess as shown by the data in Fig. 6) in reaction mixtures containing 50 mM Hepes pH 7.5, 0.1% Triton X-100, 5 mM MgCl(2), 50 mM NaCl, and 25 µM of one of the following lipids: lanes 1, [4`-P]lipid IV(A) (2 times 10^4 dpm/nmol); lanes 2, Kdo-[4`-P]lipid IV(A) (2 times 10^4 dpm/nmol); lanes 3, (Kdo)(2)-[4`-P]lipid IV(A) (1 times 10^4 dpm/nmol). The reactions were incubated at 30 °C for 30 min with (+) or without(-) 25 µM lauroyl-ACP. Arrows indicate the positions of migration of the substrates and of product a, the predominant monoacylated derivative generated from lauroyl-ACP and (Kdo)(2)-[4`-P]lipid IV(A).



At 20 µg/ml enzyme, a trace of acylation is observed with both [4`-P]lipid IV(A) and Kdo-[4`-P]lipid IV(A) (faint bands in lanes 1+ and 2+ of Fig. 8just ahead of the major component). These minor products are not seen in the absence of lauroyl-ACP (Fig. 8, lanes 1- and 2-). A trace of what may be a diacylated derivative of (Kdo)(2)-[4`-P]lipid IV(A) is also observed in Fig. 8(lane 3+), just ahead of product a. Whether these minor products represent alternative acylations catalyzed by HtrB itself or are the result of other contaminating acyltransferases will have to be re-evaluated with HtrB preparations purified to homogeneity. In crude extracts of wild-type cells, a rapid second acylation of (Kdo)(2)-[4`-P]lipid IV(A) is observed(10) , but this is catalyzed by the msbB gene product(22) . (^2)MsbB is absent in the strain employed here for the overexpression and partial purification of HtrB.

Additional Characterization of Acylated Product a

In previous work with crude extracts, we demonstrated that product a had the mass expected for (Kdo)(2)-lipid IV(A) acylated with a single lauroyl group(10) . The site of laurate attachment is expected to be the same as in lipid A isolated from cells(7, 8) , but this point remains to be established. Treatment of product a with aqueous triethylamine at 37 °C results in the release of two R-3-hydroxymyristoyl chains, as judged by thin layer chromatography (data not shown). This behavior is consistent with the attachment of the lauroyl residue to one of the N-linked R-3-hydroxymyristoyl groups, since attachment to one of the O-linked R-3-hydroxymyristoyl groups would prevent its release by aqueous triethylamine(13, 36, 37) .


DISCUSSION

Although previous studies of htrB have strongly suggested that this gene has a role in envelope assembly(17, 18, 19, 20, 21) , the current findings offer the first direct evidence that HtrB is an enzyme of lipid A biosynthesis. Given the pleiotropic nature of the htrB mutant phenotype, Karow and co-workers considered several possibilities for HtrB function, including roles in peptidoglycan(17) , fatty acid(20) , and lipopolysaccharide biogensis (19, 20, 21) . Although they did not propose a primary defect in the Kdo-dependent lauroyl transferase documented in the present work, several earlier observations support our enzymatic data. Specifically, the striking reduction in the laurate content of the lipopolysaccharide isolated from htrB deficient E. coli(20) and the reduced number of acyl chains attached to the lipid A of htrB deficient H. influenzae(38) are in accordance with our findings.

MsbB and htrB display significant sequence similarity to each other(19) , consistent with related catalytic roles (22) .^2MsbB and htrB have no homology to lpxA(39, 40) and lpxD(31) , the products of which are the R-3-hydroxymyristoyl transferases that function in the initial stages of lipid A assembly(3, 5) . All four enzymes are absolutely dependent upon acyl chain activation by ACP, since coenzyme A thioesters do not serve as substrates(10, 31, 39) . Consequently, they may share some common structural motifs. This issue is currently of considerable interest, since the x-ray crystal structure of LpxA (the first enzyme of the lipid A pathway) has recently been solved at 2.6 Å(41) . A unique feature of LpxA is its unusual left-handed parallel beta helix domain and trimeric subunit structure(41) . HtrB and MsbB do not possess the sequence repeat associated with the left-handed parallel beta helix region(41, 42, 43) , but this does not necessarily rule out the possibility of a beta helical domain. Imperfect, parallel right-handed beta helices (with no obvious associated sequence repeats) have been reported(44) .

HtrB and MsbB, unlike LpxA (45) and LpxD(31) , are membrane proteins. X-ray crystallography may not be feasible. In previous studies, however, we reported that half of the lauroyl transferase activity was in the cytoplasmic fraction(10) . Different E. coli strains and buffers were employed. Solubilization and purification of HtrB without detergents deserves further exploration.

Both htrB and msbB are present in the Haemophilus genome and show 70% identity with their E. coli counterparts(46) . HtrB and msbB display no sequence homology to other well characterized acyltransferases, such as glycerol-3-phosphate acyltransferases (46) or N-myristoyl transferases(47) . The eucaryotic acyloxyacyl hydrolase (48) that removes the acyl chains incorporated into lipid A by HtrB and MsbB is also unrelated, as judged by its sequence.

The significance of the phenotypes associated with insertions in htrB(17, 19) and the possible functions of multi-copy suppressors of htrB mutations (19, 21) must be re-evaluated in light of our findings. The increased resistance of these mutants to deoxycholate and the stimulation of their growth at non-permissive temperatures by low levels of cationic detergents (19) may be attributed to changes in lipid A structure. The morphological changes at elevated temperatures(17, 19) , such as the bulging of the cell surface that resembles the response to certain beta-lactams, are more difficult to explain. One intriguing scenario is that underacylated lipid A precursors might accumulate in the inner membrane and inhibit the export of peptidoglycan precursors, preventing peptidoglycan assembly. The msbA multi-copy suppressor of htrB (a putative transporter resembling mammalian mdr proteins) (21) might be the proposed lipid A flippase of the inner membrane(2, 5, 49, 50) . The notion of a lipid A translocation function for MsbA is supported by the recent discovery that the mdr-2 protein of the mouse is required for phosphatidylcholine secretion into bile(51) . It is conceivable that the overexpression of msbA in htrB-deficient mutants might remove excess, underacylated lipid A precursors by accelerating their export to the outer membrane.

Alternatively, MsbA could be an inner membrane translocase specific for peptidoglycan precursors(52) . Its overexpression in the setting of htrB mutations might overcome any inhibitory effects of underacylated lipid A precursors on peptidoglycan export. Further characterization of MsbA function is of great interest, since neither the lipid A nor the peptidoglycan translocases have been identified by biochemical or genetic criteria.

The function of lipid A in Gram-negative bacteria is unknown(1, 2, 5) . Strains with insertion mutations in the genes encoding the early steps of lipid A assembly cannot be grown under any condition(53, 54) . Wild-type E. coli lipid A contains two normal fatty acids in acyloxyacyl linkage (Fig. 1). Lipid As of some other Gram-negative bacteria possess only one acyloxyacyl unit(1, 2, 4) . An important implication of the present studies is that the usual acyloxyacyl moieties of E. coli lipid A are not absolutely essential for growth. Strains with htrB insertion mutations can grow slowly on nutrient media below 33 °C or at all temperatures on minimal media(17, 19) . Cells with insertion mutations in msbB show myristate-deficient lipid A and are not conditionally lethal(20, 22, 55) . Such mutants still possess the laurate-containing acyloxyacyl group generated by HtrB.

To evaluate the significance of acyloxyacyl moieties more thoroughly, a detailed analysis of the molecular species of lipid A that remain in growing htrB mutants of E. coli and in the presence of various suppressors will be of considerable interest. The msbB high multi-copy suppressor of htrB(19) may be functioning by allowing the synthesis of the myristate-containing acyloxyacyl unit that is not made in wild-type cells prior to HtrB catalyzed laurate incorporation (Fig. 1). In addition, palmitate-containing acyloxyacyl moieties can be generated by transacylation of palmitate from the 1 position of a glycerophospholipid to lipid A precursors(28) . The latter pathway is of minimal significance in wild-type cells. It is stimulated under conditions of limited lipid A biosynthesis(5, 13, 56) . It is possible that a deficiency in laurate incorporation due to an htrB mutation is compensated for by increased palmitate transfer. The further biochemical characterization of htrB mutations and their various suppressors should greatly enhance our understanding of lipid A biogenesis and function.


FOOTNOTES

*
This research was supported by National Institutes of Health Grant GM-51310 (to C. R. H. R.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
To whom correspondence should be addressed. Tel.: 919-684-5326; Fax: 919-684-8885.

(^1)
The abbreviations used are: ACP, acyl carrier protein; bis-tris, 2-[bis(2-hydroxyethyl)amino]2-(hydroxymethyl)-propane-1,3-diol.

(^2)
T. Clementz, J. J. Bednarski, and C. R. H. Raetz, manuscript in preparation.


ACKNOWLEDGEMENTS

We thank Dr. Margaret Karow for providing the htrB and msbB related strains listed in Table 1and for many helpful discussions.


REFERENCES

  1. Morrison, D. C., and Ryan, J. L. (eds) (1992) Bacterial Endotoxic Lipopolysaccharides, Vol. I: Molecular Biochemistry and Cellular Biology , CRC Press, Boca Raton, FL
  2. Raetz, C. R. H. (1990) Annu. Rev. Biochem. 59, 129-170 [CrossRef][Medline] [Order article via Infotrieve]
  3. Raetz, C. R. H. (1993) J. Bacteriol. 175, 5745-5753 [Medline] [Order article via Infotrieve]
  4. Rietschel, E. T., Kirikae, T., Schade, F. U., Mamat, U., Schmidt, G., Loppnow, H., Ulmer, A. J., Zähringer, U., Seydel, U., Di Padova, F., Schreier, M., and Brade, H. (1994) FASEB J. 8, 217-225 [Abstract/Free Full Text]
  5. Raetz, C. R. H. (1996) in Escherichia coli and Salmonella: Cellular and Molecular Biology (Neidhardt, F. C., ed) Vol. 1, Second Ed., American Society for Microbiology, Washington, D. C., in press
  6. Qureshi, N., Cotter, R. J., and Takayama, K. (1986) J. Microbiol. Methods 5, 65-77
  7. Qureshi, N., Takayama, K., Mascagni, P., Honovich, J., Wong, R., and Cotter, R. J. (1988) J. Biol. Chem. 263, 11971-11976 [Abstract/Free Full Text]
  8. Karibian, D., Deprun, C., and Caroff, M. (1993) J. Bacteriol. 175, 2988-2993 [Abstract]
  9. Myers, K. R., Ulrich, J. T., Qureshi, N., Takayama, K., Wang, R., Chen, L., Emary, W. B., and Cotter, R. J. (1992) Bioconjugate Chem. 3, 540-548 [Medline] [Order article via Infotrieve]
  10. Brozek, K. A., and Raetz, C. R. H. (1990) J. Biol. Chem. 265, 15410-15417 [Abstract/Free Full Text]
  11. Rick, P. D., and Osborn, M. J. (1977) J. Biol. Chem. 252, 4895-4903 [Medline] [Order article via Infotrieve]
  12. Rick, P. D., Fung, L. W.-M., Ho, C., and Osborn, M. J. (1977) J. Biol. Chem. 252, 4904-4912 [Abstract]
  13. Raetz, C. R. H., Purcell, S., Meyer, M. V., Qureshi, N., and Takayama, K. (1985) J. Biol. Chem. 260, 16080-16088 [Abstract/Free Full Text]
  14. Strain, S. M., Armitage, I. M., Anderson, L., Takayama, K., Qureshi, N., and Raetz, C. R. H. (1985) J. Biol. Chem. 260, 16089-16098 [Abstract/Free Full Text]
  15. Kohara, Y., Akiyama, K., and Isono, K. (1987) Cell 50, 495-508 [Medline] [Order article via Infotrieve]
  16. Borodovsky, M., Koonin, E. V., and Rudd, K. E. (1994) Trends Biochem. Sci. 19, 309-313 [CrossRef][Medline] [Order article via Infotrieve]
  17. Karow, M., Fayet, O., Cegielska, A., Ziegelhoffer, T., and Georgopoulos, C. (1991) J. Bacteriol. 173, 741-750 [Medline] [Order article via Infotrieve]
  18. Karow, M., and Georgopoulos, C. (1991) Mol. Microbiol. 5, 2285-2292 [Medline] [Order article via Infotrieve]
  19. Karow, M., and Georgopoulos, C. (1992) J. Bacteriol. 174, 702-710 [Abstract]
  20. Karow, M., Fayet, O., and Georgopoulos, C. (1992) J. Bacteriol. 174, 7407-7418 [Abstract]
  21. Karow, M., and Georgopoulos, C. (1993) Mol. Microbiol. 7, 69-79 [Medline] [Order article via Infotrieve]
  22. Clementz, T., Bednarski, J., and Raetz, C. R. H. (1995) FASEB J. 9, A1311 [Abstract/Free Full Text]
  23. Miller, J. R. (1972) Experiments in Molecular Genetics , Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
  24. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. (eds) (1989) Current Protocols in Molecular Biology , John Wiley & Sons, New York
  25. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and Klenk, D. C. (1985) Anal. Biochem. 150, 76-85 [Medline] [Order article via Infotrieve]
  26. Brozek, K. A., Hosaka, K., Robertson, A. D., and Raetz, C. R. H. (1989) J. Biol. Chem. 264, 6956-6966 [Abstract/Free Full Text]
  27. Belunis, C. J., and Raetz, C. R. H. (1992) J. Biol. Chem. 267, 9988-9997 [Abstract/Free Full Text]
  28. Brozek, K. A., Bulawa, C. E., and Raetz, C. R. H. (1987) J. Biol. Chem. 262, 5170-5179 [Abstract/Free Full Text]
  29. Hampton, R. Y., and Raetz, C. R. H. (1992) Methods Enzymol. 209, 466-475 [Medline] [Order article via Infotrieve]
  30. White, K. A., and Raetz, C. R. H. (1995) FASEB J. 9, A1376
  31. Kelly, T. M., Stachula, S. A., Raetz, C. R. H., and Anderson, M. S. (1993) J. Biol. Chem. 268, 19866-19874 [Abstract/Free Full Text]
  32. Jackowski, S., Jackson, P. D., and Rock, C. O. (1994) J. Biol. Chem. 269, 2921-2928 [Abstract/Free Full Text]
  33. Rock, C. O., and Cronan, J. E., Jr. (1979) J. Biol. Chem. 254, 7116-7122 [Medline] [Order article via Infotrieve]
  34. Post-Beittenmiller, D., Jaworski, J. G., and Ohlrogge, J. B. (1991) J. Biol. Chem. 266, 1858-1865 [Abstract/Free Full Text]
  35. Clementz, T., and Raetz, C. R. H. (1991) J. Biol. Chem. 266, 9687-9696 [Abstract/Free Full Text]
  36. Takayama, K., Qureshi, N., Mascagni, P., Anderson, L., and Raetz, C. R. H. (1983) J. Biol. Chem. 258, 14245-14252 [Abstract/Free Full Text]
  37. Qureshi, N., Takayama, K., and Ribi, E. (1982) J. Biol. Chem. 257, 11808-11815 [Abstract/Free Full Text]
  38. Lee, N. G., Sunshine, M. G., Engstrom, J. J., Gibson, B. W., and Apicella, M. A. (1995) J. Biol. Chem. 270, 27151-27159 [Abstract/Free Full Text]
  39. Anderson, M. S., and Raetz, C. R. H. (1987) J. Biol. Chem. 262, 5159-5169 [Abstract/Free Full Text]
  40. Coleman, J., and Raetz, C. R. H. (1988) J. Bacteriol. 170, 1268-1274 [Medline] [Order article via Infotrieve]
  41. Raetz, C. R. H., and Roderick, S. L. (1995) Science 270, 997-1000 [Abstract]
  42. Vaara, M. (1992) FEMS Microbiol. Lett. 97, 249-254 [CrossRef]
  43. Dicker, I. B., and Seetharam, S. (1992) Mol. Microbiol. 6, 817-823 [Medline] [Order article via Infotrieve]
  44. Yoder, M. D., and Jurnak, F. (1995) FASEB J. 9, 335-343 [Abstract/Free Full Text]
  45. Anderson, M. S., Bull, H. G., Galloway, S. M., Kelly, T. M., Mohan, S., Radika, K., and Raetz, C. R. H. (1993) J. Biol. Chem. 268, 19858-19865 [Abstract/Free Full Text]
  46. Fleischmann, R. D., Adams, M. D., White, O., Clayton, R. A., Kirkness, E. F., Kerlavage, A. R., Bult, C. J., Tomb, J.-F., Dougherty, B. A., Merrick, J. M., McKenney, K., Sutton, G., FitzHugh, W., Fields, C., Gocayne, J. D., Scott, J., Shirley, R., Liu, L.-I., Glodek, A., Kelley, J. M., Weidman, J. F., Phillips, C. A., Spriggs, T., Hedblom, E., Cotton, M. D., Utterback, T. R., Hanna, M. C., Nguyen, D. T., Saudek, D. M., Brandon, R. C., Fine, L. D., Fritchman, J. L., Furhmann, J. L., Geohagen, N. S. M., Gnehm, C. L., McDonald, L. A., Small, K. V., Fraser, C. M., Smith, H. O., and Venter, J. C. (1995) Science 269, 496-512 [Medline] [Order article via Infotrieve]
  47. Gordon, J. L., Duronio, R. J., Rudnick, D. A., Adams, S. P., and Gokel, G. W. (1991) J. Biol. Chem. 266, 8647-8650 [Free Full Text]
  48. Hagen, F. S., Grant, F. J., Kuijper, J. L., Slaughter, C. A., Moomaw, C. R., Orth, K., O'Hara, P. J., and Munford, R. S. (1991) Biochemistry 30, 8415-8423 [Medline] [Order article via Infotrieve]
  49. Osborn, M. J. (1979) in Bacterial Outer Membranes (Inouye, M., ed) pp. 15-34, Wiley, New York
  50. McGrath, B. C., and Osborn, M. J. (1991) J. Bacteriol. 173, 649-654 [Medline] [Order article via Infotrieve]
  51. Oude Elferink, R. P. J., Ottenhoff, R., van Wijland, M., Smit, J. J. M., Schinkel, A. H., and Groen, A. K. (1995) J. Clin. Invest. 95, 31-38 [Medline] [Order article via Infotrieve]
  52. Park, J. T. (1987) in Escherichia coli and Salmonella typhimurium (Neidhardt, F., ed) Vol. I, pp. 663-671, ASM Publications, Washington, D. C.
  53. Young, K., Silver, L. L., Bramhill, D., Cameron, P., Eveland, S. S., Raetz, C. R. H., Hyland, S. A., and Anderson, M. S. (1995) J. Biol. Chem. 270, 30384-30391 [Abstract/Free Full Text]
  54. Beall, B., and Lutkenhaus, J. (1987) J. Bacteriol. 169, 5408-5415 [Medline] [Order article via Infotrieve]
  55. Somerville, J. E., Jr., Cassiano, L., Bainbridge, B., Cunningham, M. D., and Darveau, R. P. (1996) J. Clin. Invest. 97, 359-365 [Abstract/Free Full Text]
  56. Takayama, K., Qureshi, N., Mascagni, P., Nashed, M. A., Anderson, L., and Raetz, C. R. H. (1983) J. Biol. Chem. 258, 7379-7385 [Abstract/Free Full Text]

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