©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
Structural Determinants in the Platelet-derived Growth Factor -Receptor Implicated in Modulation of Chemotaxis (*)

(Received for publication, August 22, 1995; and in revised form, November 2, 1995)

Koutaro Yokote (§) Seijiro Mori (1) Agneta Siegbahn (2) Lars Rönnstrand Christer Wernstedt Carl-Henrik Heldin Lena Claesson-Welsh

From the  (1)Ludwig Institute for Cancer Research, Box 595, Biomedical Center, S-751 24 Uppsala, Sweden, the Second Department of Internal Medicine, Chiba University School of Medicine, Chiba 260, Japan, and the (2)Department of Clinical Chemistry, University Hospital, S-751 85 Uppsala, Sweden

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

Activation of the platelet-derived growth factor (PDGF) beta-receptor leads to cell growth and chemotaxis. The PDGF alpha-receptor also mediates a mitogenic signal, but fails to induce cell migration in certain cell types. To examine this difference in signal transduction, a series of point-mutated PDGF alpha-receptors were analyzed. Porcine aortic endothelial cells expressing mutant PDGF alpha-receptors, in which tyrosine residues 768, 993, or 1018 were changed to phenylalanine residues migrated toward PDGF, whereas wild-type alpha-receptors and mutant alpha-receptors changed at tyrosine residues 720, 944, or 988 failed to migrate. All mutant receptors were mitogenically active and their capacity to activate phosphatidylinositol 3`-kinase and phospholipase C- was not different from that of the wild-type receptor. Tyr-768 was found to be phosphorylated in PDGF-stimulated cells; in the Y768F mutant, there was a considerable increase in phosphorylation of Ser-767. Tyr-993 was not phosphorylated, but mutation of this tyrosine residue to a phenylalanine residue resulted in increased efficiency of phosphorylation on Tyr-988. Tyr-1018 is known to be an autophosphorylation site. Phosphorylated Tyr-768 and Tyr-1018 may bind signal transduction molecules involved in negative modulation of the chemotactic signaling capacity, whereas phosphorylated Tyr-988 may mediate increased chemotaxis. Thus our data indicate that the PDGF alpha-receptor has an intrinsic ability to transduce a chemotactic signal, and that this signal is counteracted by overriding negative signals.


INTRODUCTION

Platelet-derived growth factor (PDGF) (^1)is a potent mitogen for mesenchymal cells. PDGF plays important roles in the embryonal development and in wound healing, as well as in the development of several pathological conditions, such as tumorigenesis and atherosclerosis (reviewed in (1) and (2) ). PDGF is a homo- or heterodimeric protein composed of A- and B-polypeptide chains which elicits its effects by binding to cell surface receptors designated alpha- and beta-receptors(3, 4, 5) . The PDGF alpha-receptor is able to bind either A- or B-chains of PDGF, whereas the beta-receptor binds only the B chain.

Binding of PDGF activates the intrinsic tyrosine kinase activity of the receptors through receptor dimerization, which leads to receptor autophosphorylation and to phosphorylation of intracellular substrates (6) . An important role for receptor autophosphorylation is to present binding sites for signal transduction molecules containing one or two copies of so called Src homology 2 (SH2) domains, a conserved non-catalytic stretch of about 100 amino acids found in signal transduction molecules, and which mediates the interaction with the receptor(7) . Nine and three autophosphorylation sites have so far been identified in the PDGF beta- and alpha-receptors, respectively. Several of these have been shown to interact in a specific manner with certain SH2 domain-containing proteins (reviewed in (8) ). For the PDGF beta-receptor, two autophosphorylation sites in the juxtamembrane domain (Tyr-579 and Tyr-581) mediate the binding of Src family kinases. There are four autophosphorylation sites in the kinase insert which bind Grb2 (Tyr-716), the regulatory subunit (p85) of phosphatidylinositol 3`-kinase (PI 3-kinase; Tyr-740 and Tyr-751), Nck (Tyr-751), and the GTPase activating protein (GAP) of Ras (Tyr-771). The two autophosphorylation sites in the carboxyl-terminal tail (Tyr-1009 and Tyr-1021) mediate binding of the SH2 domain-containing phosphatase PTP1D/Syp and phospholipase C- (PLC-), respectively. The adapter proteins Shc and Shb seem to interact with multiple tyrosine residues (9, 37) . In the case of the PDGF alpha-receptor, tyrosine residues 754, 988, and 1018 have been identified as autophosphorylation sites(10, 11) . Among them, Tyr-1018 has been shown to mediate association of PLC-(11) . Tyr-731 and Tyr-742 are important for the binding of PI 3-kinase(12) , and are likely to be phosphorylation sites, although this has not been directly shown.

Both PDGF alpha- and beta-receptors mediate mitogenic signals upon ligand stimulation, probably through multiple parallel signal transduction pathways(13, 14, 15) . On the other hand, the migratory response of PDGF beta-receptor expressing cells is critically dependent on activation of PI 3-kinase(16, 17) . Although the PDGF alpha-receptor also activates PI 3-kinase, this receptor mediates migration in a cell type-specific manner. Thus, Swiss 3T3 cells and human granulocytes expressing endogenous PDGF alpha-receptors (18, 19) and 32D cells expressing the PDGF alpha-receptors after transfection (12) allows chemotaxis toward PDGF-AA. In other cell types, such as human foreskin fibroblasts(20) , human monocytes(19) , rat and baboon vascular smooth muscle cells(21) , PDGF-AA fails to induce chemotaxis. In these cell types, which express both PDGF alpha- and beta-receptors, PDGF-AA has also been shown to inhibit chemotaxis induced by PDGF-AB or -BB(19, 22) . The mechanisms underlying the cell type-specific effect of the PDGF alpha-receptor on chemotaxis is still unknown.

Expression of the PDGF beta-receptor after transfection in porcine aortic endothelial (PAE) cells, which lack endogenous PDGF receptors, allow the cells to migrate toward PDGF-BB, whereas PAE cells expressing the PDGF alpha-receptor do not migrate toward PDGF-AA or -BB(23) , thus offering a suitable model for analysis of the molecular mechanisms responsible for alpha-receptor-induced suppression of chemotaxis.

Here, we show that mutations of three tyrosine residues in the alpha-receptor allows the transduction of a chemotactic response of PAE cells expressing the mutant alpha-receptors. Characterization of the pattern of autophosphorylation in these mutants implicated the presence of multiple pathways mediated via the PDGF alpha-receptor which control cellular chemotaxis.


EXPERIMENTAL PROCEDURES

Cell Culture and Mutagenesis

Site-directed mutagenesis was performed on a cDNA encoding the full-length human PDGF alpha-receptor (24) using the Altered sites in vitro Mutagenesis System (Promega Corp.). The following oligonucleotides were used for the mutagenesis: 5`-GAAAGCACACGGAGCTTTGTTATTTTATCTTTT-3` (Y720F), 5`-GATCGTCCAGCCTCATTTAAGAAGAAATCTATG-3 (Y768F), 5`-AGTGAGATTCTCGAGAATCTG-3 (Y944F), 5`-GACAATGCATTCATTGGTGTC-3 (Y988F), 5`-GGTGTCACCTTCAAAAACGAGG-3` (Y993F), and 5`-GACAGTGGCTTCATCATTCCTC-3 (Y1018F). All mutations were confirmed by DNA sequencing. The wild-type and the mutated cDNAs were inserted into the eukaryotic expression vectors pZip Neo or into pcDNA3 (Invitrogen). The constructs were transfected into PAE cells (25) by electroporation and subsequently selected for neomycin resistance as described previously(24) . PAE cells expressing the wild-type PDGF beta-receptors have been described earlier(24) . I-PDGF-BB binding assays and Scatchard analysis were performed according to Mori et al.(27) . For serum starvation, subconfluent cultured cells were incubated in Ham's F-12 medium containing 0.5% fetal calf serum for 16 h.

Antibodies

The rabbit antiserum PDGFR-3, specifically recognizing the PDGF beta-receptor (26) and PDGFR-7, recognizing the PDGF alpha-receptor, have been described earlier(23) . The rabbit antisera YSD and SQT were raised against synthetic peptides corresponding to amino acid residues 754-769 and 1042-1061, respectively, of the human PDGF alpha-receptor. The mixed monoclonal antibodies against bovine PLC- were purchased from Upstate Biotechnology Inc. The monoclonal antibody against RasGAP was purchased from Santa Cruz Biotechnology. The monoclonal anti-phosphotyrosine antibody PY20 was from Affiniti Research Products Ltd. Peroxidase-conjugated sheep anti-mouse immunoglobulins were from Amersham Corp.

[^3H]Thymidine Incorporation Assay

The ability of PDGF-BB to stimulate DNA synthesis, measured by the incorporation of [^3H]thymidine, in the different transfected cell lines was performed as described previously (26) .

Chemotaxis Assay

The migration assay was performed in a modified Boyden chamber essentially as described before(19) . Micropore filters (thickness; 150 µm, pore size; 8 µm) coated with type-1 collagen solution at 100 µg/ml (Vitrogen 100; Collagen Corp.) were used in the assay. Cells were trypsinized and resuspended in Ham's F-12 medium supplemented with 10% FCS at the final concentration of 3 times 10^5/ml. The cell suspension was placed in the upper chamber and the medium containing various doses of PDGF-BB in Ham's F-12, 10% FCS was placed below the filter in the lower chamber. For each set of experiments, the migration of the respective cell-clone in Ham's F-12, 10% FCS above and below the filter (stimulated random migration or chemokinesis) served as control and is referred to as 100% migration. All experiments were performed in duplicate for every concentration of PDGF-BB and at least two independent cell clones from wild-type or each type of mutated receptors were analyzed. All the assays were read in blind.

Immunoprecipitation and Immunoblotting

Serum-starved cells were treated with PDGF-BB for 1 h on ice, rinsed with ice-cold phosphate-buffered saline (PBS), and then lysed in the Nonidet P-40 lysis buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% Nonidet P-40, 2.5 mM EDTA, 500 µM Na(3)VO(4), 1% aprotinin (Trasylol; Bayer) and 1 mM phenylmethylsulfonyl fluoride (Sigma). Immunoprecipitation with rabbit antiserum or monoclonal antibody was for 2 h at 4 °C, followed by incubation with protein A-Sepharose CL-4B (Pharmacia) for 30 min at 4 °C. The samples were eluted by boiling in SDS sample buffer (4% SDS, 0.2 M Tris-HCl, pH 8.8, 0.5 M sucrose, 5 mM EDTA, 0.01% bromphenol blue, 2% 2-mercaptoethanol), and then separated by SDS-PAGE. After electrophoretic transfer onto a nitrocellulose membrane (Hybond-C extra, Amersham), the blot was blocked in 5% bovine serum albumin, 0.2% Tween 20 in PBS at 4 °C overnight, probed with PY20 (dilution 1:2500) or anti-PLC- antibody (1:5000) followed by incubation with horseradish peroxidase-linked anti-mouse IgG (sheep; Amersham; 1:1000); immunocomplexes were visualized through enhanced chemiluminescence (ECL; Amersham). The blots were reprobed after removal of the first probe by incubation in 5 mM sodium phosphate, pH 7.5, 2% SDS, and 2 mM 2-mercaptoethanol for 30 min at 60 °C.

In Vitro Kinase Assay

After ligand treatment, the cells were lysed and Immunoprecipitation was performed with PDGFR-3 or PDGFR-7 antiserum. The immunoprecipitated samples were subjected to kinase assays, analyzed by SDS-PAGE and autoradiography as described by Yokote et al.(9) .

PI 3-Kinase Assay

Serum-starved cells were treated with 50 ng/ml PDGF-BB at 37 °C for 5 min, rinsed with an ice-cold buffer containing 20 mM Hepes, pH 7.5, 150 mM NaCl, and 100 µM Na(3)VO(4) and then lysed in the same buffer supplemented with 10% glycerol, 1% Nonidet P-40, 5 mM EDTA, 1% aprotinin, and 1 mM phenylmethylsulfonyl fluoride. Clarified cell lysates were immunoprecipitated using PDGFR-3 or PDGFR-7. The Sepharose-immune complexes were subjected to PI 3-kinase assay essentially as described by Wennström et al.(16) . Briefly, the immunoprecipitates were washed three times with PBS containing 1% Nonidet P-40, once with PBS, once with 0.1 M Tris-HCl, pH 7.5, 0.5 M LiCl, once with distilled water, and once with 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM EDTA on ice. The Sepharose beads were suspended in 50 µl of 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.5 mM EGTA, and 0.2 mg/ml phosphatidylinositol (sonicated for 15 min at 4 °C; Sigma), and preincubated at room temperature for 10 min. Ten µCi of [-P]ATP and MgCl(2) (final concentration, 10 mM) were added and the samples were further incubated for 10 min. Reactions were stopped by addition of chloroform, methanol, 11.6 M HCl (50:100:1), phospholipids were extracted with chloroform and the organic phase was washed with methanol, 1 M HCl (1:1). Reaction products were concentrated in vacuo, dissolved in chloroform, spotted on Silica Gel 60 plates (Merck) impregnated with 1% potassium oxalate, and resolved by chromatography in chloroform, methanol, 28% ammonia, water (43:38:5:7) for 45 min. Phosphorylated products were detected by autoradiography.

Measurement of Inositol Phosphate Formation

Measurement of total inositol phosphates was performed according to Kozawa et al.(28) . Cells were labeled with myo-[^3H]inositol (1.5 µCi/ml) in serum-free, inositol-free Ham's F-12 medium for 48 h. The labeled cells were washed twice with an assay buffer (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM KCl, 5.5 mM glucose, 0.8 mM MgSO(4), and 1 mM CaCl(2)) and then preincubated in the same buffer containing 0.01% bovine serum albumin and 200 mM LiCl for 20 min at 37 °C. The cells were then incubated without or with 50 ng/ml PDGF-BB or with 5% FCS, for 15 min at 37 °C. The reaction was terminated by adding 30% trichloroacetic acid. The acid supernatant was recovered, treated with diethyl ether to remove the acid, and neutralized with 0.1 M NaOH. The supernatant was applied to an anion exchange column containing 1 ml of Dowex AG1-X8 (100-200 mesh, formate form). The resin was washed with 8 column volumes of H(2)O and then with 20 volumes of a solution containing 5 mM disodium tetraborate and 60 mM sodium formate. Finally, total inositol mono-, bis-, tris-, and tetrakisphosphates were eluted from the resin by addition of 0.1 M formic acid containing 1 M ammonium formate and subjected to scintillation counting.

Covalent Coupling of Antibody to Protein A-Sepharose

The antiserum of interest was incubated with an equal volume of protein A-Sepharose (1:1 slurry) for 1 h at 4 °C. The antibody-protein A-Sepharose was washed three times with PBS, twice in 0.1 M sodium borate, pH 9.0, and then resuspended in the same solution. Dimethyl pimelimidate was added at a final concentration of 20 mM. After 30 min of incubation at room temperature, the antibody-protein A-Sepharose was washed once and incubated overnight in 0.2 M ethanolamine, pH 8.0. Then the antibody-protein A-Sepharose was washed twice with PBS, once with 50 mM glycine, pH 3.0, once with 1 M Tris-HCl, pH 7.5, twice with PBS, and then kept in PBS, 0.1% sodium azide at 4 °C.

[P]Orthophosphate Labeling, Phosphopeptide Mapping, Edman Degradation, and Phosphoamino Acid Analysis

The procedures were performed essentially according to Rönnstrand et al.(29) . Briefly, serum-starved cells were labeled by incubation in phosphate-free Ham's F-12 medium supplemented with 0.5% dialyzed fetal calf serum and 4 mCi/ml [P]orthophosphate for 3 h at 37 °C. Cells were treated with 100 ng/ml PDGF-BB in the same medium for 1 h at 4 °C, and lysed in a Triton lysis buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.5% Triton X-100, 0.5% deoxycholate, 5 mM EDTA, 100 µM Na(3)VO(4), 1% Trasylol, and 1 mM phenylmethylsulfonyl fluoride). Immunoprecipitation was performed using PDGFR-7, the samples were separated by SDS-PAGE and then transferred onto nitrocellulose membrane. For in situ tryptic digestion, the radioactively labeled PDGF alpha-receptor was cut out after exposure to film, and incubated with 0.5% polyvinylpyrrolidone 40, 0.6% acetic acid for 30 min at 37 °C. The filter pieces were rinsed three times with water and then incubated for 12 h at 37 °C with 400 µl of 50 mM ammonium bicarbonate containing 1 µg of modified sequencing-grade trypsin (Promega). The supernatant was lyophilized, oxidized in performic acid for 1 h on ice, again lyophilized, resuspended in 50 µl of ammonium bicarbonate, and incubated with 1 µg of trypsin for another 12 h at 37 °C. The samples were lyophilized, dissolved in a pH 1.9 buffer consisting of 88% formic acid, acetic acid, water (50:156:1794), and analyzed by two-dimensional phosphopeptide mapping on a cellulose plate, using electrophoretic separation at pH 1.9 in the first dimension, followed by ascending chromatography (isobutyric acid, pyridine, glacial acetic acid, H(2)O, 1-butanol, 65:5:3:29:2) in the second. Radioactive phosphopeptides were visualized by autoradiography. When necessary, phosphopeptides on plates were scraped off and then eluted with the same buffer as for the mapping. In case of immunoprecipitation of tryptic fragments, lyophilized tryptic digests were dissolved in 50 mM ammonium bicarbonate and incubated for 2 h at 4 °C with YSD-antiserum covalently coupled to protein A-Sepharose. The Sepharose was washed three times with 50 mM ammonium bicarbonate, 0.05% Triton X-100, twice with 150 mM ammonium bicarbonate, 0.5% Triton X-100, and twice with distilled water. The immunoprecipitates were then eluted with 1% diethylamine, pH 11.9. The purified peptides were coupled to a Sequelon-AA membrane (Milligen/Biosearch, Burlington, MA) according to the manufacturer's instructions, and Edman degradation was run in an Applied Biosystems gas-phase sequencer. For cyanogen bromide cleavage, the piece of nitrocellulose membrane with autophosphorylated receptors was rinsed with water and incubated with 30% formic acid containing 100 mg/ml cyanogen bromide for 12 h at 25 °C. The sample was lyophilized, dissolved in 50 mM ammonium bicarbonate, and immunoprecipitated with SQT-antiserum. The eluted material was oxidized, subjected to tryptic digestion, and analyzed by two-dimensional phosphopeptide mapping. For phosphoamino acid analysis, peptides were hydrolyzed in 6 M hydrochloric acid for 1 h at 110 °C, separated by two-dimensional electrophoresis on a cellulose plate, and then examined by autoradiography.


RESULTS

Characterization of PAE Cell Lines Expressing Y720F, Y768F, Y944F, Y988F, Y993F, and Y1018F Mutants of the PDGF alpha-Receptors

Signal transduction by receptor tyrosine kinases is dependent on interactions between autophosphorylated tyrosine residues in the receptors and SH2-domain containing intracellular signaling proteins. In the closely related PDGF alpha- and beta-receptors, certain tyrosine residues are positioned in regions conserved between the two receptors, whereas other tyrosine residues are unique for each receptor (Fig. 1). We reasoned that the uniquely positioned tyrosine residues could be involved in alpha-receptor-specific signaling, e.g. transduction of a negative effect on chemotaxis. Thus, the codons for the unique tyrosine residues 720, 768, 944, 988, 993, and 1018 of the wild-type human PDGF alpha-receptor cDNA were changed individually to phenylalanine codons by site-directed mutagenesis, generating Y720F, Y768F, Y944F, Y988F, Y993F, and Y1018F receptor mutants. PAE cells which lack endogenous PDGF receptors, were stably transfected with cDNAs for the different mutants as well as with wild-type PDGF alpha-receptor cDNA. PAE cells expressing the wild-type PDGF beta-receptors were prepared as described previously (27) . The numbers of receptors expressed per PAE cell, estimated by I-PDGF-BB binding experiments, were similar for the different wild-type and mutant receptor expressing cell lines (Table 1). Furthermore, as judged from the results of immunocomplex kinase assays, all the different mutant receptors were ligand-stimulatable kinases. Thus, the fold-induction of kinase activity in response to PDGF was the same for the wild-type and mutant receptors (Fig. 2). To further characterize the mutant receptor expressing cell lines, [^3H]thymidine incorporation assays were performed (Table 2). The mutant expressing cell types as well as the wild-type alpha-receptor cells all responded mitogenically to PDGF-BB to a similar extent. PAE cells expressing the wild-type PDGF beta-receptor showed a slightly higher mitogenic response.


Figure 1: Schematic illustration of the location of tyrosine residues in the intracellular domains of the PDGF alpha- and beta-receptors and their interacting SH2 domain-containing proteins. The numbers stand for positions of the tyrosine residues; numbers in ovals represent uniquely positioned tyrosine residues, whereas numbers in squares indicate tyrosine residues positioned in regions conserved between the two types of receptors. JM, juxtamembrane domain; TK1, first part of the tyrosine kinase domain; KI, kinase insert; TK2, second part of the tyrosine kinase domain; CT, carboxyl-terminal tail.






Figure 2: Ligand-stimulated kinase activity of the wild-type beta, wild-type alpha, and the tyrosine residue-mutated PDGF alpha-receptors. PAE cells expressing the wild-type and mutant PDGF receptors were incubated without(-) or with (+) 100 ng/ml PDGF-BB for 30 min at 4 °C. The cells were lysed and immunoprecipitated with anti-receptor antisera. The samples were subjected to kinase assays as described under ``Experimental Procedures,'' followed by SDS-PAGE, and autoradiography.





Mutations at Certain alpha-Receptor-unique Tyrosine Residues Enable the Mutant Receptors to Mediate Chemotaxis

PAE cells expressing the PDGF alpha-receptor after transfection respond mitogenically to PDGF-BB, but fail to migrate toward PDGF-BB or PDGF-AA(23) . To examine the effect of mutations at uniquely positioned tyrosine residues in the PDGF alpha-receptor on the ability to mediate a chemotactic response, PAE cells expressing the wild-type or mutant PDGF alpha-receptors were examined using a modified Boyden chamber assay. For comparison, PAE cells expressing the wild-type PDGF beta-receptors were also examined. Cells were suspended in Ham's F-12 medium containing 10% FCS and placed above an 150-µm thick filter in the Boyden chamber. Various doses of PDGF-BB in Ham's F-12, 10% FCS were added below the filters and the assays were run for 6 h at 37 °C in a humidified chamber containing 90% air and 10% CO(2). The filters were then fixed, stained, and examined by microscopy. Migration was measured as the distance of the two furthest migrating PAE cell nuclei from the origin of migration. The migration distance in each filter was calculated as the means of readings of at least three randomly chosen areas of the filter. For each experiment, the migration distance due to random migration, with Ham' s F-12, 10% FCS on both sides of the filters, served as a control and was set to 100% migration. As shown in Fig. 3, PAE cells expressing the wild-type PDGF beta-receptor, Y768F, Y993F, or Y1018F PDGF alpha-receptors (referred to as chemotactic mutants) showed a dose-dependent chemotactic response toward PDGF-BB, whereas cells expressing either wild-type PDGF alpha-receptor, mutant receptors Y720F, Y944F, or Y988F, as well as non-transfected PAE cells, showed no chemotactic response. Cell migration toward PDGF-BB of the chemotactic mutants increased up to 140% of control value, with the maximal response seen at 20 ng/ml PDGF-BB. PAE cells expressing the wild-type PDGF beta-receptor showed a maximal chemotactic response of 160%.


Figure 3: Chemotaxis of cells expressing the wild-type PDGF beta-receptors, the wild-type, or mutant PDGF alpha-receptors. Non-transfected PAE cells (down triangle) and PAE cells expressing the wild-type PDGF beta-receptor (), wild-type (), Y720F (box), Y768F (), Y944F (circle), Y988F (Delta), Y993F (bullet), or Y1018F () PDGF alpha-receptors were analyzed for their migration toward different concentrations of PDGF-BB. Stimulated random migration (chemokinesis) was estimated as described under ``Experimental Procedures'' and is referred to as 100% migration. The data show mean ± S.E. of three experiments. At least two PAE cell clones for each of the wild-type or mutant receptors were analyzed and gave similar results.



Chemotactic Mutant alpha-Receptors Mediate Activation of Known Substrates upon Receptor Activation

Wennström et al.(18) and Kundra et al.(17) showed that activation of PI 3-kinase is critical for chemotactic signaling elicited by the PDGF beta-receptor. The human PDGF alpha-receptor binds the p85 subunit of PI 3-kinase via tyrosine residues 731 and 742(12) , and the Y731F/Y742F mutant PDGF alpha-receptor expressed in 32D cells had lost its capacity to mediate ligand-induced cell movement(30) . We examined whether the ability to activate PI 3-kinase differed between the wild-type and the chemotactic mutant PDGF alpha-receptors. To perform PI 3-kinase assays, PAE cells expressing wild-type beta-, wild-type alpha-, or mutant alpha-receptors were treated with 50 ng/ml PDGF-BB for 5 min at 37 °C. The cells were lysed and the lysates were immunoprecipitated with anti-receptor antiserum. The precipitated material was characterized with regard to in vitro phosphorylation of phosphatidylinositol (PI) as assessed by liquid chromatography. As seen in Fig. 4A, the wild-type alpha- and beta-receptors and the chemotactic mutant PDGF alpha-receptors, showed similar extents of phosphatidylinositol phosphate formation upon PDGF-BB stimulation.


Figure 4: A, thin-layer chromatography of PI 3-kinase reaction products from unstimulated or ligand stimulated cells. Cells expressing the wild-type PDGF beta-receptors, wild-type PDGF alpha-receptors, Y768F, Y993F, or Y1018F mutant PDGF alpha-receptors were incubated without(-) or with (+) 50 ng/ml PDGF-BB for 5 min at 37 °C. After incubation, the cells were lysed and immunoprecipitated with anti-receptor antiserum. The immunoprecipitates were subjected to PI 3-kinase assays and the PI 3-kinase reaction products were analyzed by thin-layer chromatography followed by autoradiography. The positions of phosphatidylinositol phosphate and the origin (ORI) are indicated. B, tyrosine phosphorylation of PLC- after PDGF-BB stimulation of PAE cells expressing wild-type or tyrosine residue-mutated PDGF receptors. Cells expressing the wild-type PDGF beta-receptors, wild-type PDGF alpha-receptors, Y768F, Y993F, or Y1018F mutant PDGF alpha-receptors were incubated without(-) or with (+) 100 ng/ml PDGF-BB for 30 min at 4 °C. After incubation, the cells were lysed and immunoprecipitated with anti-PLC- monoclonal antibody (left panels) or with anti-receptor antiserum (right panel). The immunoprecipitates were separated by SDS-PAGE and transferred onto nitrocellulose membranes. The blots were probed with either anti-phosphotyrosine monoclonal antibody PY20 or with anti-PLC- antibody and then visualized using ECL Western blotting detection system (Amersham). Migration positions of the PDGF beta-receptors (betaR), PDGF alpha-receptors (alphaR), and PLC- are indicated. Density of the detected bands were measured by densitometric scanning. The relative densities of the tyrosine phosphorylated PLC-/tyrosine phosphorylated PDGF receptor for the wild-type beta-, wild-type alpha-, Y768F alpha-, Y993F alpha-, and Y1018F alpha-receptors were 1.1, 0.074, 0.095, 0.098, and 0.000, respectively. C, formation of inositol phosphates in PAE cells expressing wild-type or tyrosine-residue mutated PDGF receptors. Cells labeled with myo-[^3H]inositol (1.5 µCi/ml) for 48 h were incubated in presence of vehicle (open bars), 50 ng/ml PDGF-BB (solid bar), or 5% FCS (shaded bars) for 15 min at 37 °C. The samples were quenched by addition of 30% trichloroacetic acid. The total inositol phosphates were separated by anion exchange chromatography and the amount measured by scintillation counting. The values represent means ± S.D. of triplicate determinations.



It has been suggested that the SH2 domain-containing proteins PLC- and RasGAP are involved in modulation of the chemotactic response(17, 31) ; PLC- in a positive and RasGAP in a negative manner. We examined complex formation and tyrosine phosphorylation of these signaling proteins upon receptor activation. The different cell types were stimulated with PDGF-BB for 30 min on ice and then lysed. The lysates were immunoprecipitated either with anti-PLC- antiserum or with anti-receptor antiserum, separated by SDS-PAGE, transferred onto nitrocellulose membranes; the membranes were then immunoblotted with the anti-phosphotyrosine antibody PY20. Upon ligand stimulation of wild-type PDGF beta-receptor, wild-type alpha-receptor, and the mutant Y768F and Y993F alpha-receptors, PLC- became tyrosine phosphorylated and was found in complex with the autophosphorylated receptors. Phosphorylation of PLC- was not detected upon activation of mutant Y1018F PDGF alpha-receptors, in agreement with the recent characterization of Tyr-1018 as the major PLC- binding site in the PDGF alpha-receptor(11) . Eriksson et al.(11) also showed that a higher extent of complex formation between the receptor and PLC- is observed upon activation of the PDGF alpha-receptor compared to the beta-receptor; however, tyrosine phosphorylation and consequently activation of the catalytic activity of PLC-, is considerably more efficient in activated PDGF beta-receptor cells than in PDGF alpha-receptor cells. There was no difference in the efficiency of tyrosine phosphorylation of PLC- between the wild-type alpha-receptor and the Y768F and Y993F mutant alpha-receptors as assessed by densitometric scanning of the immunoblots in Fig. 4B. In order to examine whether the efficiency of induction of the enzymatic activity of PLC- differed between the wild-type and the chemotactic mutant alpha-receptors, PDGF-induced formation of inositol phosphates was measured (Fig. 4C). Cells were labeled with myo-[^3H]inositol for 48 h, incubated with or without 50 ng/ml PDGF-BB for 15 min at 37 °C, and the samples were extracted by addition of 30% trichloroacetic acid to the cell monolayer. Total inositol phosphates were collected using AG 1-X8 formate resin. Upon PDGF-BB stimulation, the total amount of radioactively labeled inositol phosphates increased to 160% of the control value in the wild-type beta-receptor expressing cells, while the wild-type alpha-receptor expressing cells showed only a slight increase. The difference in the level of increase in total inositol phosphates between the PDGF beta- and alpha-receptors is consistent with results previously described for primary human fibroblasts (11) and rat vascular smooth muscle cells(32) . Like in the wild-type alpha-receptor, the extent of accumulation of total inositol phosphates were negligible in the Y768F, Y993F, or Y1018F chemotactic mutant alpha-receptors. Thus, it is not likely that PLC- catalytic activity is involved in mediation of chemotaxis of the mutant alpha-receptor cells.

Upon ligand stimulation of the wild-type PDGF beta-receptor expressing cells, RasGAP was tyrosine phosphorylated and in complex with the receptor, as judged from coimmunoprecipitation of the receptor when a RasGAP antiserum was used. However, neither activation of the wild-type nor the chemotactic mutant PDGF alpha-receptors induced phosphorylation or association of RasGAP with the receptors (data not shown). This is in agreement with previous reports by Heideran et al.(33) and Bazenet et al.(34) , in which RasGAP is described to be a substrate for the PDGF beta-receptor but not for the alpha-receptor.

In conclusion, these observations argue against modulation of PDGF alpha-receptor-mediated tyrosine phosphorylation or activation of PI 3-kinase, RasGAP, or PLC-, as a reason for the gain of chemotactic ability of the Y768F, Y993F, and Y1018F mutant alpha-receptors.

Tyr-762, Tyr-768, and Ser-767 Are in Vivo Phosphorylation Sites in the PDGF alpha-Receptor

In order to investigate the molecular mechanisms for the gain of chemotactic capacity of the mutant PDGF alpha-receptors, we first examined whether Tyr-768 is an autophosphorylation site in the PDGF alpha-receptor. For this purpose, PAE cells expressing the wild-type or Y768F PDGF alpha-receptors were labeled with [P]orthophosphate, incubated with PDGF-BB, lysed, and immunoprecipitated with the anti-alpha-receptor antiserum PDGFR-7. The immunoprecipitated material was separated by SDS-PAGE, transferred onto nitrocellulose membrane which was exposed to x-ray film, whereafter the phosphorylated receptor band was excised from the membrane. The material on the membrane piece was subjected to in situ tryptic digestion and the digest was analyzed by two-dimensional phosphopeptide mapping (Fig. 5A). A radioactive phosphopeptide spot (indicated by an arrowhead) in the wild-type alpha-receptor map was lacking in the Y768F PDGF alpha-receptor map. This phosphopeptide was extracted from the TLC plate of the wild-type receptor map, and subjected to Edman degradation. As shown in Fig. 5B, radioactive peaks appeared at cycles 3 and 9. This suggests that the peptide is phosphorylated at both positions 3 and 9; the lower amount of radioactivity in cycle 9 is likely to be due to a rather low repetitive yield during the Edman degradation. Phosphoamino acid analysis of the same material revealed that the peptide was phosphorylated on tyrosine (Fig. 5B, inset). Complete trypsin digestion of the human PDGF alpha-receptor intracellular domain would be expected to give rise to around 50 different peptides. Among these, the peptide encompassing Tyr-762 and Tyr-768 is the only one containing phosphorylatable amino acids at both the third and ninth positions from the amino terminus.


Figure 5: A, two-dimensional tryptic phosphopeptide maps of the wild-type and Y768F PDGF alpha-receptors. Cells expressing the wild-type or Y768F PDGF alpha-receptors were labeled in vivo with [P]orthophosphate, stimulated with PDGF-BB, and immunoprecipitated with anti-receptor antiserum. The immunoprecipitates were separated by SDS-PAGE and transferred onto nitrocellulose membranes. The bands corresponding to the PDGF alpha-receptors were cut out, digested with trypsin in situ, oxidized, and again extensively digested with trypsin. The resulting digests were separated electrophoretically at pH 1.9, followed by ascending chromatography. Radioactive phosphopeptides were visualized by autoradiography. The position of a phosphopeptide spot present in the wild-type alpha-receptor map but not in the Y768F alpha-receptor map is indicated by an arrowhead. In each case, the origin is marked with an open triangle. B, Edman degradation elution profile and phosphoamino acid analysis (inset) of the phosphopeptide indicated by an arrowhead in the wild-type PDGF alpha-receptor map in A. The phosphopeptide was extracted from TLC plate, subjected to Edman degradation, and the P radioactivity in the fractions generated in each cycle was measured. The amino acid sequence of the tryptic peptide containing Tyr-768 is presented along with the fraction numbers. Migration positions of serine and tyrosine in the phosphoamino acid analysis are indicated as S and Y, respectively. C, elution profile by Edman degradation and phosphoamino acid analysis of the tryptic peptides from the wild-type (upper panel) and Y768F (lower panel) PDGF alpha-receptors immunoprecipitated with YSD antiserum. The amino acid sequence of the YSD-immunoprecipitated peptide is presented along with the fraction numbers. Results of the phosphoamino acid analysis on the peptides are shown on the right-hand side of each panel.



If both Tyr-762 and Tyr-768 are autophosphorylation sites, one would expect a peptide containing phosphorylated Tyr-762 to be recognized in the Y768F receptor map. This peptide would be expected to become more positively charged and hydrophobic compared to the corresponding wild-type peptide, due to replacement of the phosphorylated tyrosine residue with a phenylalanine residue. Thus, the peptide is expected to migrate more toward the right (toward the anode) upon electrophoresis, and upwards upon chromatography, compared to the wild-type peptide. In the particular experiment shown in Fig. 5A, ascending chromatography was run extensively to obtain high resolution of the Tyr-768-containing spot in the wild-type receptor map, which resulted in loss of some phosphopeptides from the TLC plate. When the peptides were run for a shorter time in the ascending chromatography, at least two phosphopeptide spots were found in the upper right corner of the Y768F receptor map but not in the wild-type receptor map (data not shown). One of the two peptides was phosphorylated on serine and radioactivity appeared at cycle 8, the other showed tyrosine and serine phosphorylation and radioactivity appeared at cycles 3 and 8, as judged from phosphoamino acid analysis and Edman degradation (data not shown). These phosphopeptides most likely represented the peptides containing Tyr-762 and Phe-768.

In order to confirm phosphorylation on tyrosine residues 762 and 768, and to further examine serine phosphorylation in the peptide, tryptic digests from the in vivoP-labeled wild-type and Y768F PDGF alpha-receptors were immunoprecipitated with an antiserum which specifically recognizes Tyr-768 and the surrounding amino acid residues (YSD antiserum). The immunoprecipitates were eluted and subjected to Edman degradation. As shown in Fig. 5C, upper panel, radioactive peaks were detected at cycles 3, 8, and 9 in the wild-type receptor fragment, corresponding to Tyr-762, Ser-767, and Tyr-768. Phosphoamino acid analysis of the immunoprecipitated peptide revealed phosphorylation strongly on tyrosine and weakly on serine. In the corresponding analysis of the Y768F PDGF alpha-receptor (Fig. 5C, lower panel), peaks of phosphorylation were seen at cycles 3 and 8, which corresponded to Tyr-762 and Ser-767. Radioactivity in cycle 9 most likely represents trailing from cycle 8. The result of phosphoamino acid analysis of the immunoprecipitated Y768F peptide revealed a dramatic increase in the ratio of serine phosphorylation to tyrosine phosphorylation compared to that of the corresponding peptide from the wild-type receptor. The ratios of the radioactive content of cycle 8 to cycle 3 were 0.26 for the wild-type peptide and 1.57 for the Y768F peptide, which suggested that substitution of Tyr-768 to a phenylalanine residue resulted in considerable enhancement of phosphorylation on Ser-767.

Replacement of Tyr-993 with a Phenylalanine Residue Enhances Autophosphorylation at Tyr-988

We next analyzed whether Tyr-993 is autophosphorylated in vivo. In a two-dimensional analysis of a tryptic digest of the Y993F alpha-receptors generated after in vivoP labeling as described above, there was no difference as compared to the pattern of the wild-type alpha-receptor tryptic digest suggesting that Tyr-993 is not an autophosphorylation site (Fig. 6A). This is in agreement with previous observations(11) . However, a spot of high intensity showed up in the Y993F map (Fig. 6A, arrow). Extraction of the corresponding radioactive phosphopeptide followed by Edman degradation showed a peak of radioactivity at the seventh cycle (Fig. 6B). The peptide was mainly phosphorylated on tyrosine as judged from phosphoamino acid analysis (Fig. 6B, inset). There are four possible tryptic fragments from the PDGF alpha-receptor containing a tyrosine residue at the seventh position from the amino terminus: one fragment from the juxtamembrane domain containing Tyr-613 (Fig. 6B, a), one from the kinase insert containing Tyr-742 (Fig. 6B, b), and two from the carboxyl-terminal tail containing Tyr-988 and Tyr-1018, respectively (Fig. 6B, c and d). Tyr-1018 has previously been shown to be autophosphorylated in vivo(14) . Two-dimensional analysis of a tryptic digest from the Y1018F mutant receptor, after in vivo labeling, allowed identification of two spots representing the Tyr-1018-containing peptide (Fig. 6A, arrows). The location of the intense spot in the Y993F map was clearly different from the Tyr-1018-containing peptide spots.


Figure 6: A, two-dimensional tryptic phosphopeptide maps of the wild-type, Y993F, and Y1018F PDGF alpha-receptors. Cells expressing the wild-type, Y993F, or Y1018F PDGF alpha-receptors were labeled in vivo with [P]orthophosphate, stimulated with PDGF-BB, and immunoprecipitated with anti-receptor antiserum. The immunoprecipitates were separated by SDS-PAGE and transferred onto nitrocellulose membranes. The bands corresponding to the PDGF alpha-receptors were cut out, digested with trypsin in situ, oxidized, and again extensively digested by trypsin. The resulting digests were separated electrophoretically at pH 1.9, followed by ascending chromatography. Radioactive phosphopeptides were visualized by autoradiography. An intense spot evident in the Y993F map is indicated by an arrow. Arrowheads indicate the positions of the phosphopeptides containing Tyr-1018. In each case, the origin is marked with an open triangle. B, Edman degradation elution profile and phosphoamino acid analysis (inset) of the phosphopeptide indicated by the arrow in the Y993F map in A. The phosphopeptide was extracted from a TLC plate, subjected to Edman degradation, and the P radioactivity in the fractions generated in each cycle was measured. The amino acid sequences of all the possible PDGF alpha-receptor-derived tryptic peptides, containing a tyrosine residue in the seventh position, are presented along with the fraction numbers. Asterisk (*) indicates the Phe-993 residue.



We argued that Tyr-988 could be phosphorylated to an increased extent in the Y993F PDGF alpha-receptor, because of the closeness of these residues. To examine this possibility, the following experiment was performed. The wild-type, Y988F, Y993F, and Y1018F PDGF alpha-receptors were labeled in vivo, autophosphorylated, and immunoprecipitated according to the procedure described above. The receptor proteins were then chemically cleaved with cyanogen bromide resulting in peptide fragments of relatively large sizes. A group of fragments from the carboxyl-terminal tail of the receptors (amino acid residues 981 to 1072) were collected by immunoprecipitation using a specific antiserum raised against amino acid residues 1042 to 1061 of the alpha-receptor (SQT antiserum). The immunoprecipitated fragments were further digested by incubation with trypsin and then analyzed by two-dimensional phosphopeptide mapping. The tryptic map derived from the immunoprecipitated cyanogen bromide fragment showed five radioactive phosphopeptide spots (Fig. 7A), designated spots 1 to 5 (Fig. 7B). In the map from Y1018F alpha-receptors, spots 1 and 2 were missing as compared to the wild-type alpha-receptor map. Edman degradation of peptides 1 and 2 in the wild-type receptor map showed tyrosine phophorylation at the seventh amino acid from the amino terminus (data not shown), in agreement with the notion that the peptides contained phosphorylated Tyr-1018. The spots 3, 4, and 5 were lacking in the Y988F alpha-receptor map, suggesting that these spots in the wild-type alpha-receptor map represented peptides containing Tyr-988. Interestingly, the radioactive content of spots 4 and 5 from the Y993F alpha-receptor was 50% higher as compared with the corresponding spots derived from the wild-type alpha-receptor. The radioactive contents of spots 1 and 2 were the same in the two cases. These results indicate that Tyr-988 is phosphorylated with increased stoichiometry following mutation of Tyr-993 to a phenylalanine residue, without affecting the phosphorylation efficiency on Tyr-1018.


Figure 7: A, two-dimensional tryptic phosphopeptide maps of cyanogen bromide-cleaved fragments derived from the carboxyl-terminal tail of wild-type, Y988F, Y993F, and Y1018F PDGF alpha-receptors. Cells expressing the wild-type, Y988F, Y993F, or Y1018F PDGF alpha-receptors were labeled in vivo with [P]orthophosphate, stimulated with PDGF-BB, and immunoprecipitated with anti-receptor antiserum. The immunoprecipitates were separated by SDS-PAGE and transferred onto nitrocellulose membranes. The band corresponding to the PDGF alpha-receptor was cut out, cleaved with cyanogen bromide, and immunoprecipitated with SQT antiserum. The immunoprecipitated fragment was oxidized and digested with trypsin. The resulting digests were separated electrophoretically at pH 1.9, followed by ascending chromatography. Radioactive phosphopeptides were visualized by Bio-imaging analyzer BAS 2000 (Fuji photo film). The origin of each sample has been trimmed away in the figure, but is located outside the lower-left corner of each panel. B, quantification of radioactivity in the phosphopeptide spots in the tryptic maps in A. The intensities of the phosphopeptide spots (designated spots 1 to 5) in A were measured using BAS 2000. In order to compare the intensity values of the spots between the different receptor maps, the value for spot number 1 was fixed to 100 in each map. Intensities of other spots were calculated relative to the values for spot number 1. Arrows indicate the recorded major changes in phosphorylation.




DISCUSSION

It is well established that PDGF beta-receptor expressing cells migrate efficiently toward PDGF-BB. Data based on beta-receptors lacking the PI 3-kinase binding site(16, 17) , as well as treatment with the PI 3-kinase inhibitor wortmannin and expression of a mutated PI 3-kinase p85 subunit, (^2)indicate that PI 3-kinase activity is crucial for beta-receptor-mediated chemotaxis. The ligand-stimulated PDGF alpha-receptor also activates PI 3-kinase, but fails to induce chemotaxis in certain cell types. We show that point-mutated alpha-receptors can acquire the capacity to mediate chemotaxis of PAE cells, in which the wild-type alpha-receptor is chemotactically inactive. Our data imply that the lack of wild-type alpha-receptor-mediated chemotaxis in the PAE cells and other cell types is not due to a lack of ability to activate the signal transduction pathways leading to directed migration. Rather, one or more negative signals are also activated by the alpha-receptor which suppress the migratory response. Since PI 3-kinase was active in the ligand-stimulated chemotactic mutants, the negative signals are likely to act downstream of PI 3-kinase. PLC-, which has been described to be involved in positive regulation of chemotaxis in certain cell types (16, 17) is most likely not responsible for modulation of alpha-receptor-mediated chemotaxis, since the Y1018F mutant, which is unable to bind and activate PLC-, was able to mediate chemotaxis. RasGAP has been inferred to negatively modulate PDGF beta-receptor mediated chemotaxis(17) . Neither the wild-type, nor the chemotactically active mutant alpha-receptors, associated with or phosphorylated RasGAP, arguing against a role for RasGAP in alpha-receptor signaling.

The PDGF alpha-receptor can thus emit both positive and negative signals influencing the migratory response. The capacity to emit these signals appear to depend on the cellular environment. Interestingly, the negative signals can affect migration initiated by other ligand-receptor complexes. We have previously shown that activation of the PDGF alpha-receptor leads to inhibition of PDGF beta-receptor-mediated chemotaxis, in a dose-dependent manner(19) . Koyama et al.(21, 22) described that activation of the PDGF alpha-receptor in vascular smooth muscle cells led to inhibition of chemotaxis induced by the PDGF beta-receptor, or by receptors for fibronectin and smooth muscle cell-derived growth factor. It is likely that the negative signals acting on the alpha-receptor-induced chemotaxis and on chemotaxis induced by other types of receptors are the same.

Koyama et al.(21, 22) showed that PDGF alpha-receptor-induced inhibition of fibronectin-mediated chemotaxis was attenuated by treatment of the vascular smooth muscle cells with the protein kinase C (PKC) inhibitor staurosporine suggesting that PKC is a mediator of PDGF alpha-receptor negative signaling. However, another PKC inhibitor H-7, and the PKC activators 12-O-tetradecanoylphorbol-13-acetate and SC-9, had no effect in their study, which together with the rather low specificity of staurosporine for PKC(35) , may suggest involvement of a different serine/threonine kinase in the negative signaling.

Diliberto et al.(36) showed that PDGF-BB (activating both alpha- and beta-receptors) was more potent than PDGF-AA (activating only alpha-receptors) in stimulating Ca fluxes in Balb/c3T3 fibroblasts. In addition, preincubation of the cells with PDGF-AA lead to inhibition of the PDGF-BB-induced increase in the intracellular Ca levels, in part through PKC-dependent mechanisms. It is an interesting possibility, which remains to be tested, that differences in the modulation of intracellular Ca levels account for the differences in chemotactic signaling between the wild-type alpha-receptor and alpha-receptor mutants.

Our data indicate that several mechanisms are operating in negative modulation of PDGF alpha-receptor negative signaling. Phosphorylation of Tyr-768, which is surrounded by the motif PASY768KKK, appears to allow negative modulation of chemotaxis, maybe by binding a specific signal transduction molecule. This signal transduction molecule would be activated by the alpha-receptor but not by the beta-receptor, since there is no tyrosine residue with a similar environment in the beta-receptor. It is, however, also possible that the gain of chemotactic ability of the Y768F alpha-receptor mutant was due to the increased phosphorylation of Ser-767 in the mutant receptor expressing cells, which could lead to positive modulation of chemotaxis. Analysis of a double mutant, simultaneously changed at Ser-767 and Tyr-768, will allow us to differentiate between these two alternatives. Tyr-762 was also shown to be an autophosphorylation site in our study. Although this residue is a positional homologue of Tyr-771 in the beta-receptor, which is the binding site for RasGAP, the alpha-receptor does not bind RasGAP. Distinct surrounding motifs for the two tyrosine residues, RSLY762DRP in the alpha-receptor and SSNY771MAP in the beta-receptor, probably account for the difference in binding of RasGAP. The alpha-receptor Tyr-993 is not phosphorylated, but we could show that there was increased phosphorylation of Tyr-988 in the Y993F mutant receptor expressing cells. Tyr-988 is surrounded by the motif DNAY988IGV which is not found in the beta-receptor. It is possible that the increased phosphorylation of Tyr-988 in the Y993F mutant receptor cells allows positive modulation of chemotaxis, by a more efficient recruitment of a specific signal transduction molecule.

The mechanism by which the Y1018F alpha-receptor mutant allowed chemotaxis is not clear. It is not likely that the loss of PLC- binding resulted in the chemotactic phenotype, since PLC- has been shown to act positively (17) or to be without effect (16) on beta-receptor-mediated chemotaxis. Moreover, activation of the catalytic activity of PLC- is not prominent in ligand-stimulated wild-type or mutant alpha-receptor expressing PAE cells (Fig. 5) or in other cell types expressing endogenous receptors, such as vascular smooth muscle cells (32, 35) or human foreskin fibroblasts(11) . It is possible that a signal transduction molecule distinct from PLC- can also bind to phosphorylated Tyr-1018 and mediate the negative signal for chemotaxis.

The different potential pathways for modulation of PDGF alpha-receptor-induced chemotaxis are summarized in Fig. 8. We suggest that autophosphorylated tyrosine residues 768 and 1018 serve to mediate negative regulation of chemotaxis, downstream of PI 3-kinase. In contrast, it is possible that Tyr-988 mediates a signal for positive regulation. The negative signaling for modulation of chemotaxis appears to act on the level of directed migration only, since both membrane edge ruffling, which can be regarded as an integral part of the cellular motility response(16) , as well as random migration, were induced to the same level in ligand-stimulated wild-type and mutant alpha-receptor PAE cells, as in the wild-type PDGF beta-receptor expressing cells. (^3)This is in agreement with the observation by Koyama et al.(21) that PDGF-AA inhibited PDGF-BB-induced chemotactic activity but not random migration of baboon smooth muscle cells. Identification of the signal transduction molecules that interact with the phosphorylation sites determined in this work, Tyr-768 and Tyr 988, and elucidation whether molecules other than PLC- interact with phosphorylated Tyr-1018, may reveal the basis for the cell-type specific pattern of alpha-receptor negative signaling. Such efforts should also broaden our understanding of how directed movement of cells is controlled.


Figure 8: Schematic illustration of possible modes of modulation of chemotactic signaling by the PDGF alpha-receptor. Numbers indicate the positions of tyrosine residues. (P) indicates that the tyrosines are phosphorylatable. +, positive modulation; -, negative modulation; PI3-K, phosphatidylinositol 3`-kinase. Mutation of the phosphorylatable tyrosine residues 768 or 1018 to phenylalanine residues relieves a putative negative regulatory mechanisms, whereas mutation of Tyr-993 causes an increased autophosphorylation of Tyr-988 possibly resulting in an enhanced chemotactic signaling.




FOOTNOTES

*
This work was supported in part by the Ax:son-Johnson foundation and by a grant from the Swedish Cancer Society (to A. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
To whom correspondence should be addressed. Tel.: 46-18-551688; Fax: 46-18-506867.

(^1)
The abbreviations used are: PDGF, platelet-derived growth factor; PAE cells, porcine aortic endothelial cells; PI 3-kinase, phosphatidylinositol 3`-kinase; PLC-, phospholipase C-; GAP, GTPase activating protein; SH2, Src homology 2; PAGE, polyacrylamide gel electrophoresis; PKC, protein kinase C; FCS, fetal calf serum; PBS, phosphate-buffered saline.

(^2)
S. Wennström, L. Claesson-Welsh, R. Hooshmand-Rad, K. Yokote, C.-H. Heldin, and A. Siegbahn, unpublished observation.

(^3)
K. Yokote, C.-H. Heldin, and L. Claesson-Welsh, unpublished observation.


ACKNOWLEDGEMENTS

We thank Matilda Johnell for expert technical assistance in the chemotaxis assay, Ulla Engström for peptide synthesis, Anders Eriksson for providing Y988F, Y993F, and Y1018F PDGF alpha-receptor cDNA, Osamu Kozawa for helpful advice on inositol phosphates measurement, and Ingegärd Schiller for skillful secretarial assistance.


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