©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
High Frequency and Error-prone DNA Recombination in Ataxia Telangiectasia Cell Lines (*)

(Received for publication, July 18, 1995; and in revised form, December 18, 1995)

Chen-Mei Luo Wei Tang Kristin L. Mekeel Jeffrey S. DeFrank P. Rani Anné Simon N. Powell (§)

From the Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

The only specific DNA repair defect found in ataxia telangiectasia (A-T) cells is mis-repair of cleaved DNA. In this report we measured DNA recombination, given its role in DNA repair and genetic instability. Using plasmids containing selectable reporter genes, we found a higher frequency of both chromosomal recombination (>100 times) and extra-chromosomal recombination (27 times) in SV40-transformed A-T cell lines compared with in an SV40-transformed normal fibroblast cell line. Southern analysis of single A-T colonies exhibiting post-integration recombination revealed that 24/27 had undergone aberrant rearrangements; recombination in normal fibroblast colonies was achieved by gene conversion in 8/11 clones and 10/11 clones showed unchanged copies of the plasmid. Using co-transfection of two integrating plasmids, each containing a separate deletion in the xgprt reporter gene, the 27 times difference in extra-chromosomal recombination was found when the plasmids were cleaved at a distance from the reporter gene. When the plasmids were cleaved within the reporter gene, the co-transfection frequency was reduced in A-T, but was increased in normal cells. We conclude that A-T cell lines have not only a high frequency chromosomal and extra-chromosomal recombination, but also exhibit error-prone recombination of cleaved DNA.


INTRODUCTION

Ataxia telangiectasia (A-T) (^1)is an autosomal recessive disorder characterized by progressive cerebellar ataxia, skin changes, endocrine disorders, immune defects, and high risk of cancer. A-T cell lines have abnormalities in DNA damage processing including hypersensitivity to ionizing radiation (1) and radiation-resistant DNA synthesis(2, 3) . The A-T gene function appears to be central to DNA damage processing, cell cycle checkpoints, and genomic stability; interest in determining the function of the gene comes from many disciplines.

Following localization of the A-T gene to 850 kb on the long arm of chromosome 11(4, 5) , it now appears to have been cloned(6) . The surprising result is that one gene is mutated in all the complementation groups, which makes the original observations of complementation groups difficult to rationalize. The cloned sequences have features in common with a phosphatidylinositol 3-kinase and the rad 3 gene of Schizosaccharomyces pombe. How loss of function of this gene achieves the pleiotropic phenotype remains unsolved.

Progress in understanding this complex syndrome has been restricted to extensive definition of the phenotype(7) . One of the main puzzling aspects of the A-T response to ionizing radiation is the lack of a gross defect in DNA double-strand break repair. Recently it has been suggested that all or most of the ionizing radiation sensitivity of A-T is due to early triggering of programmed cell death by otherwise non-lethal DNA damage(8) . Another view of the A-T gene function is a lack of damage surveillance, which may help explain both radiosensitivity and lack of cell-cycle arrest in response to ionizing radiation. The role of the A-T gene in the damage signaling pathway was suggested by the lack of or delayed rise in p53 following ionizing radiation(9, 10, 11, 12) . However, the models proposed by both Meyn (8) and Thacker (7) are incongruous: if the damage signaling pathway is at fault, how is apoptosis triggered so readily? Signaling of damage appears sufficient to stimulate known response markers such as poly(A)DPribose(13) .

Although A-T cells have no gross defect in closing double-strand breaks, they have been shown to have a high frequency of double-strand break mis-repair (14, 15, 16) using plasmid reactivation. A high rate of spontaneous intra-chromosomal, but not extra-chromosomal, recombination has also been described using a plasmid recombination substrate(17) . Our previous observations had suggested that mis-repair may be linked to recombination, and a working hypothesis was that double-strand breaks from ionizing radiation trigger recombination, which in A-T is error prone. Thus, although strand breaks are closed readily, residual damage is present which leads to mitotic cell death or apoptosis. The cell-cycle arrest response to ionizing radiation may be more complex than first described, and was recently shown to be cell-cycle phase dependent(18) . The A-T gene may highlight a difference in the signal pathway of cell-cycle arrest and apoptosis.

In this report we transfected plasmids, designed to evaluate DNA recombination/repair events, into A-T and normal cell lines. Specifically, we measured chromosomal and extra-chromosomal recombination; whether cleavage of the recombination substrates affected the outcome; and the type of recombination events seen in A-T compared with normal cells.


MATERIALS AND METHODS

Cell Lines and Tissue Culture

The cell lines, AT2SF (GM09607) and AT5BIVA (GM5849A), are SV40-transformed fibroblast cell lines from patients with ataxia telangiectasia. NF(GM00637F) is an SV40-transformed normal human fibroblast cell line. All cells were grown in Dulbecco's modification of Eagle's medium with 10% (v/v) fetal bovine serum and containing penicillin (100 units/ml) and streptomycin (100 mg/ml). Cell cultures were incubated in 5% CO(2) at 37 °C. The cells were grown in monolayer culture and were detached when required using trypsin (0.05%) and versene (0.02%).

Vectors

The construction of pTPSN has been described(19) . In brief, it is a hybrid sequence and contains three antibiotic resistance genes (Fig. 1A), a neomycin (neo) resistance gene used to select for stable transformants flanked by two mutant genes encoding hygromycin resistance (hyg). The mutations in the hygromycin genes were made by an insertion of a HindIII linker at a different site in each gene. The first insertion mutation was introduced into the hyg gene at the unique PvuI site and the second at a SacII site. Recombination between the two defective genes can lead to a functional hygromycin gene. The two mutant genes are distinguishable because the PvuI mutant hyg gene is recoverable as a 2.9-kb DraI fragment, whereas the SacII mutant hyg gene is recoverable as a 2.6-kb DraI fragment. The presence or absence of the HindIII site within the DraI fragment will determine whether the gene is unchanged or has undergone gene conversion, respectively. Exchange between the two mutant genes, detected by Southern analysis, results in a 2.5-kb band on DraI digestion, and no HindIII site. Using only HindIII digestion for rapid screening of clones, unchanged plasmid produces a 6- and 4-kb band; gene conversion leads to a 10-kb band; and exchange produces an 8- or a 12-kb band.


Figure 1: A, map of plasmid pTPSN linearized at the ClaI site showing relevant restriction enzyme sites. The linear plasmid is 10.2 kb. Two mutant hygromycin genes (hyg) are enclosed within different sized DraI fragments: a HindIII insertion at SacII site cuts the 2.6-kb DraI fragment into 1.4- and 1.2-kb bands; a HindIII insertion at PvuI site cuts the 2.9-kb fragment into 1.7- and 1.2-kb bands. There are two HindIII fragments: a 4-kb HindIII fragment containing the single ClaI site and the remaining 6-kb HindIII fragment contains the neomycin resistance gene (neo). The shaded areas represent the gene promoter regions. The solid black line represents the HindIII linker insert. The arrows show the direction of transcription. B, BamHI; C, ClaI; D, DraI; H, HindIII. Relevant restriction fragment lengths in kb are shown. B, map of plasmid pSV2gpt showing the deletions within the xanthine-guanine-phosphoribosyl transferase gene (xgprt) which give rise to the pDelta2 and pDelta3 plasmids. pDelta2 has the PvuII-HindIII fragment deleted; pDelta3 has the EcoRV-BglI fragment deleted: the deleted parts of the gene are shaded. Between the sites of deletion in pDelta2 and pDelta3, there are 460 base pairs of identical sequence, where recombination will lead to a functional xgprt gene. The KpnI and EcoRV restriction enzyme sites are within the xgprt gene and the EcoRI restriction enzyme site is distant from the xgprt gene by 2 kb in either direction.



The plasmids pDelta2,pDelta3 (20) were derived from pSV2gpt(21) and are shown in Fig. 1B. These plasmids contain the bacterial amp gene and the gpt gene with a deletion. The bacterial gpt gene with flanking SV40 promoter and processing sequences, confers resistance to mycophenolic acid. pDelta2 lacks the SV40 promoter and the first 120 base pairs of the gene coding sequence, whereas pDelta3 lacks the 3` end of the gpt gene. There are 460 base pairs between the deleted sequences, retained in both deletion plasmids, containing the single KpnI site. A single EcoRI restriction enzyme site is distant from the gpt coding region. The deletion plasmids do not have an intact gpt gene, but if pDelta2+pDelta3 are co-transfected into cells, reconstitution of the gpt gene can occur via inter-molecular recombination.

pTPSN Transfection and Isolation of G418-resistant Clones

Twenty micrograms of linear pTPSN were transfected into each flask containing 2 times 10^6 A-T or NF cells using the calcium phosphate co-precipitation method. pTPSN was linearized at its unique ClaI site before transfection to facilitate integration of the two mutant hygromycin genes. Following 4-6 h incubation with plasmid DNA, 48-60 h was allowed for growth and expression before harvesting and reseeding three flasks with 10^4, 10^5, and 10^6 cells. Three 100-mm dishes were also seeded with 10^4 and 10^5 cells for the isolation of single cell derived clones. Initial selection used 0.5 mg/ml G418. After 14-21 days growth in G418, with medium changed every 7 days, visible and viable colonies were marked and counted.

Single G418-resistant (neo^R) colonies were isolated and amplified with and without G418. In parallel, the clones were tested for hygromycin sensitivity and only hygromycin-sensitive clones were used for further testing. The initial cell count at the time of establishing hygromycin sensitivity and the final cell count following amplification, prior to seeding in hygromycin, were used to calculate the recombination frequency per cell generation over which recombination events could accumulate(22) , taking no account of cell loss following cell division, but correcting for plating efficiency. Mixed G418-resistant (neo^R) colonies were also harvested and counted: if the cell number was insufficient for immediate seeding, amplification with or without G418 was allowed. The recombination frequency was corrected for cell generation based on the total number of G418-resistant colonies harvested and the calculated amplification of cells prior to seeding in hygromycin. When there were sufficient cells amplified from either single or multiple G418-resistant colonies, 10^4, 10^5, and 10^6 cells were seeded into T75 flasks in medium with or without G418, and 50 µg/ml hygromycin was added the next day.

After 14-21 days growth in hygromycin, visible colonies (hyg^R) were stained with methylene blue. The frequency of hygromycin resistance per viable cell was recorded. A minimum of three flasks per seeding density and two different seeding densities were used per data point. Single neo^R and hyg^R colonies were isolated and transferred from dishes to small wells and maintained with the same selective medium until DNA extraction.

pDelta2,pDelta3 Co-transfection

The plasmids pSV2gpt, pDelta2, and pDelta3, where indicated, were cut at their KpnI or EcoRV sites within the gpt gene or the EcoRI site at a distance from the gpt gene, with 2 units of enzyme per µg of plasmid before transfection. Agarose gel electrophoresis was used to confirm cleavage. Ten micrograms of each of pDelta2 and pDelta3 or 20 µg of pSV2gpt were used per flask containing 2 times 10^6 cells in each experiment. Transfections were achieved using calcium phosphate co-precipitation as previously, with selection using XHATM medium containing xanthine (10 µg/ml), hypoxanthine (13.6 µg/ml), aminopterin (0.176 µg/ml), thymidine (3.87 µg/ml), and mycophenlic acid (10 µg/ml). After 14-21 days growth in XHATM, visible colonies were stained with methylene blue. Transfection frequency was defined as number of colonies per seeded viable cell. A minimum of three flasks per seeding density and two different seeding densities were used per data point. The transfection frequency of pDelta2 and pDelta3, relative to the transfection frequency of pSV2gpt represents the efficiency of recombination.

Southern Blot Analysis of pTPSN Transfections

The plasmid DNA content of the neo^R and hyg^S or hyg^R clones was determined by Southern blot analysis. DNA was isolated, using detergent lysis and phenol then chloroform extractions, and 10 µg of DNA were digested by restriction endonucleases. Probes were prepared by random priming of the 2.2-kb BamHI fragment of pTPSN containing the hygromycin gene and radiolabeling with deoxycytidine 5`-[alpha-P]triphosphate (DuPont NEN).


RESULTS

Chromosomal Recombination

Recombination between the two hyg genes seen following stable integration was measured in three single-cell derived neo^R clones of AT5 and NF (see Table 1A). All clones were shown to be sensitive to hygromycin at the time of cloning. The geometric mean recombination frequency following stable integration was 40.7 times 10 per cell generation in AT5 and 0.25 times 10 per cell generation in NF, 163 times more frequent in A-T cells (p < 0.001). Selection in hygromycin alone compared with selection in G418 and hygromycin did not alter the result (see Table 1B; AT5-C7 = 100.2 and 94.2 times 10 per cell generation, respectively; NF-C6 = 0.31 and 0.23 times 10 per cell generation, respectively). It might be expected that maintaining G418 selection would preclude the mechanism of reciprocal exchange, since the neo gene is deleted by this mechanism. The relative proportion of reciprocal exchange recombination must be low because selection in hygromycin with or without G418 did not affect the recombination frequency. When the single-cell derived culture was re-cloned again, the recombination frequency was unchanged (AT5-C6 = 19.1; NF-C6 = 0.15; NF-C1 = 0.18 times 10 per cell generation) and selection in hygromycin with or without G418 did not alter the result.



Transfection frequencies for pTPSN and recombination frequencies for mixed colonies are shown in Table 2. The transfection frequencies (neo^R colonies per viable cell) in AT5(BIVA), AT2(SF), and NF were not significantly different. The geometric mean rate (± S.E.) of spontaneous chromosomal recombination observed directly following transfection (hyg^R colonies per viable neo^R cell) in AT5 was 378 (245-582) times 10 per cell generation, 126 times more frequent than NF, which was 2.99 (1.6-5.6) times 10 per cell generation (p < 0.001). The recombination rate for AT2 was 250 (119-525) times 10 per cell generation, 84 times more frequent than NF (p < 0.001). These data are not corrected for pTPSN copy number, which did not differ significantly and would not account for the differences between A-T and NF (see below and Table 4). Recombination frequencies were increased, 9.3 times in AT5 and 11.8 times in NF, compared with the recombination frequency of stable integrated sequences ( Table 1versusTable 2). At first sight, this might imply that recombination occurring extra-chromosomally or with integration is not significantly different between A-T and normal cells.





Extra-Chromosomal, Inter-Molecular Recombination

The co-transfection frequency of pDelta2 and pDelta3 and the transfection frequency of pSV2gpt are shown in Table 3. The relative transfection frequency of pDelta2 and pDelta3 compared with pSV2gpt gives a measure of the efficiency of recombination, which accounts for both frequency and accuracy of recombination. EcoRI cleaved the plasmids outside the gpt gene; conversely, KpnI cleaved within the gpt gene. The comparison between KpnI and EcoRI was to evaluate the impact of cleavage within the recombination substrate; in other words, to assess the impact of DNA double-strand breaks on DNA recombination. When the plasmids were cleaved at a distance from the gpt gene, the co-transfection frequency was 18.3 times 10 in AT5 and 0.68 times 10 in NF, a 27-fold increased extra-chromosomal recombination frequency in A-T cells. The pSV2gpt transfection frequency was similar (41.8 times 10 in AT5 and 43.9 times 10 in NF). Thus, the co-transfection frequency of pDelta2 and pDelta3 was 44% of the transfection frequency of linear pSV2gpt in AT5, but only 1.5% in NF. When the plasmids were cleaved within the gpt gene, the co-transfection frequency was 6.33 and 2.1 times 10 in AT5 and NF, respectively. The co-transfection frequency was reduced 3-fold in A-T but was increased 3-fold in NF, resulting in a final difference between the cells of only 3-fold. The extra-chromosomal recombination frequency can appear to be little different between A-T and normal cells depending on the site of cleavage and the recombination substrate. A-T cells are adversely affected by cleavage within the recombination substrate, whereas NF cell recombination is enhanced. It is concluded that DNA cleavage promotes errors in recombination in A-T cells, and that extra-chromosomal recombination is more frequent in A-T cells.



pTPSN Plasmid Processing

Single-cell derived hyg^R clones were analyzed to establish copy number and to evaluate the processing of transfected sequences for evidence of gene conversion, exchange, other rearrangement, or no sequence alteration. Clones demonstrating each type of processing are shown in Fig. 2. Analysis of 8 A-T and 9 NF G418-resistant clones showed the copy number to vary from 1 to 10, in the expected range for transformed fibroblast lines, with no difference between A-T and NF.


Figure 2: Processing of pTPSN to achieve hygromycin resistance. The right-hand lanes show plasmid only marker lanes with digestion by either HindIII or DraI or both; each pair shows 1 copy and 5 copies. When the plasmid remains unchanged, HindIII digestion results in 6- and 4-kb bands; DraI digestion leads to a 2.9- and 2.6-kb bands; and digestion by both enzymes leads to 1.7-, 1.4-, and 1.2-kb bands. Three representative clones are shown: each clone has 3 lanes reflecting HindIII, DraI, and HindIII + DraI digestion. Gene conversion leads loss of the HindIII site within either copy of the hyg gene, resulting in the 2.9-kb or the 2.6-kb band being resistant to HindIII (AT5 and AT2 clones). Reciprocal exchange results in a 2.5-kb DraI fragment which is HindIII resistant (NF clone). Other rearrangements leading to hygromycin resistance can occur and result in bands of different size with the HindIII insertion mutation removed.



Clones derived from the A-T and NF cell lines, which were resistant to hygromycin, are shown in Table 4, with a significant proportion of these clones shown in Fig. 3. The plasmid copy number in AT5 and NF was not different. AT2 clones had a single copy in 11/14 analyzed, which was lower than AT5 or NF and may explain why the recombination frequency was marginally lower in AT2 compared with AT5. However, differences in copy number cannot explain the differences in recombination frequency between A-T and NF. There was no detectable difference in the type of plasmid processing seen in clones containing a single copy of pTPSN compared with clones with multiple in tandem copies. For example, single copy status of clone C57 (AT5) was established by BamHI digest, which revealed a 2.2-kb band and one further larger band containing the other hygromycin gene (see Fig. 4A). Hygromycin sensitivity was confirmed by a failure to grow in selective media and cleavage of all DraI fragments by HindIII. Following the development of hygromycin resistance, the 2.6-kb DraI fragment has been lost, and a 3.1-kb DraI fragment developed. It is presumed that the combination of newly developed bands (3.1-kb DraI fragment; 1.9-kb DraI/HindIII fragment) accounts for hygromycin resistance, although the retention of a HindIII site in the DraI fragment is not usually found. A sequential analysis of AT5 clone C58, which contains 2 full copies of the plasmid and a further partial copy, is shown in Fig. 4B. Before selection with hygromycin, 2.9 and 2.6-kb DraI fragments are seen, which cleave with HindIII to the 1.7-, 1.4-, and 1.2-kb fragments. Additional DraI bands (5.4 kb and 3.1 kb) are seen which are both cleaved by HindIII. Following the development of hygromycin-resistance in a clone growing out of the previously cloned cell culture, the following changes are apparent: the loss of the 2.9- and 2.6-kb bands, the development of a HindIII resistance in the 3.1-kb band, and the new development of HindIII-resistant 3.7- and 1.8-kb DraI bands. Thus, in both single copy and multiple(2, 3) copy transfected clones, the development of post-integration aberrant rearrangements are a characteristic feature of A-T cells, not seen in normal cells. Hygromycin-resistant clones were obtained from multiple independent G418-resistant cultures to avoid the analyzed clones being siblings. The results showed remarkable consistency within cell type and difference between cell types; no effect of independent culture could be found.


Figure 3: A, the processing of pTPSN in 10 hygromycin-resistant clones of A-T cells (see Table 4). Two types of restriction enzyme digestion of genomic DNA were analyzed for each single cell-derived hygromycin-resistant clone: the left-hand lane of the pair shows HindIII + DraI digestion; the right lane shows DraI digestion alone. Clones C7 and W4 show gene conversion of the PvuI mutant hygromycin gene (2.9 kb, HindIII resistant). C7 shows marked amplification in addition to conversion and aberrant rearrangements. W4 also shows retention of the HindIII site in some copies of the hyg gene. C54 shows reciprocal exchange with a 2.5-kb band which is HindIII resistant, together with other bands which are abnormal but retaining the HindIII site. All other lanes show an abnormal sized DraI fragment (of sufficient length to contain a functional gene) with loss of the HindIII site. The presence of multiple faint bands may be caused by heterogeneity arising during expansion of cell number: an indicator of the instability of the genome in A-T cells. B, the processing of pTPSN in 8 hygromycin-resistant clones of NF cells. 7/8 clones show unaltered restriction-enzyme digestion patterns in one or more copies of the plasmid, together with gene conversion of one copy of the 2.9-kb DraI fragment in six of these seven clones. W5 also shows gene conversion of the 2.6-kb DraI fragment. The clone C6, which has lost all normal sized bands, showed an abnormal rearrangement with large DraI fragment (not shown).




Figure 4: A, sequential analysis of a G418-resistant clone which starts as sensitive to hygromycin and develops resistance. Single copy status is suggested by the BamHI digestion pattern (2.2-kb band and a second larger band). Both DraI fragments are cleavable by HindIII initially. As hygromycin resistance develops, a 3.1-kb DraI band appears in place of the 2.6-kb band. Curiously, the band was still HindIII cleavable, and the resultant 1.9-kb band is sufficiently large to contain the uninterrupted hygromycin sequence. B, sequential analysis of a hygromycin-sensitive clone containing 2-3 copies of pTPSN. Initial integration has generated aberrant DraI bands of 5.4 and 3.1 kb, and two normal copies of the 2.9- and 2.6-kb DraI fragments, all of which cleave with HindIII. The development of hygromycin resistance results in loss of the 2.9- and 2.6-kb DraI fragments, the development of HindIII resistance in the 3.1-kb band, the new development of aberrant sized HindIII-resistant DraI fragments of 3.7 and 1.8 kb, and the aberrant 5.4-kb DraI fragment is further rearranged.



Hygromycin-resistant NF clones retained at least one or more unaltered copies of the mutant hyg genes in 10/11 clones; 8/11 clones achieved hygromycin resistance via gene conversion of a single copy; 1/11 demonstrated an exchange event; 1/11 demonstrated an abnormal rearrangement and one exhibited hygromycin resistance without a clear etiology. By marked contrast, A-T clones retained unchanged sequences in 2/27 clones; 10/27 showed evidence of gene conversion; and 24/27 demonstrated complex rearrangements, in which hygromycin resistance was not a result of gene conversion or exchange in at least 17 of these 24 clones. The clone, C3, developed faint HindIII-resistant DraI bands, seen only on a later film, which suggested heterogeneity developing in the clone during the short period of amplification and presumably reflects the enhanced genetic instability of A-T cells. The clone W8 had no apparent hygromycin containing bands, despite keeping the cells in hygromycin until extracting DNA. Thawing the frozen cells from this clone revealed the cells to have re-developed hygromycin sensitivity. The suggestion is that intrinsic instability allowed the rapid development of the loss of hygromycin resistance genes. The intrinsic recombinational processes (and presumably repair) appear to be sequence altering in A-T and sequence conserving in normal cells. Rearrangements that fail to reconstitute a functional gene cannot be measured by these assays.


DISCUSSION

A-T cells show hypersensitivity to ionizing radiation which has been assumed to be due to a deficiency in DNA repair. In this paper, we have found A-T cells to have an abnormally high rate of recombination and to have abnormal, error-prone recombination in assays of chromosomal and extra-chromosomal recombination. The role of recombination in double-strand break repair and radiation sensitivity has been demonstrated recently in xrs cells (23) and scid cells(24, 25) . Both cell types have reduced or absent V(D)J recombination and a failure to close DNA double-strand breaks. By contrast, A-T cells have normal double-strand break closure but also demonstrate frequent chromosome aberrations(5, 26) . It is suggested that error-prone strand break processing may underlie the sensitivity to ionizing radiation in A-T.

Hyper-recombination

The only previous report of hyper-recombination in A-T cells emphasized that high frequency recombination was restricted to intra-chromosomal events, and that extra-chromosomal recombination was not different from normal. The data presented in this paper supports hyper-recombination in both chromosomal and extra-chromosomal sites. Transfection frequency did not differ between A-T and normal cells in this or other reports(14, 15, 16, 17) . However, when recombination between extra-chromosomal substrates was required to result in a stable integrated gene, we found a higher frequency of recombination in A-T. It is not clear how the relative frequency of recombination in this assay (pDelta2 and pDelta3) should compare with the relative frequency of intra-chromosomal recombination by pTPSN. The rate of recombination measured by the extra-chromosomal assay is limited by the overall transfection frequency, whereas the intra-chromosomal assay is restricted by only the rate of recovery of recombination events, determined by the plasmid, the extent of homology, the number of copies and possibly the site of integration.

The site of cleavage in relation to the sequences required to recombine appears to be important. If the DNA termini are within or close to the recombination substrates, then the high frequency of recombination in A-T is offset by mis-repair of the recombining sequences. Previous studies have found up to 5-fold differences in recombination of extra-chromosomal plasmid DNA, which has been recorded as not significant(16, 27, 28, 29, 30) . The results seen with co-transfection of KpnI-cleaved plasmid could be due to extra-chromosomal rejoining between the 3` end of pDelta2 and the 5` end of pDelta3 rather than recombination. However, the same results are obtained when only one of the two plasmids is cleaved, or when pDelta2 is cleaved with EcoRV and pDelta3 is cleaved with KpnI, which precludes a simple re-ligation mechanism (data not shown). It is also concluded that A-T cells show recombination mediated mis-repair around the site of strand breakage.

Recombination: Before Integration, With Integration, or Post-integration?

The recombination frequency associated with integration of pTPSN was approximately 10 times higher than that for stable intra-chromosomal plasmid sequences. Selection for hygromycin was only applied after G418 selection and not immediately following transfection. Integration enhances recombination by unknown mechanisms, perhaps by inducing strand breakage.

Recombination of vectors such as pDelta2/pDelta3 is generally thought to be extra-chromosomal, but also requires an integration step. Recombination may occur by events occurring extra-chromosomally, associated with integration, or intra-chromosomally. Our data with pDelta2/pDelta3 and the previously reported data cannot separate solely extra-chromosomal events from events associated with integration, even though these assays have been widely reported to reflect extra-chromosomal recombination. The assay of V(D)J recombination evaluates entirely extra-chromosomal events, and A-T cells have been shown to make functional coding and signal joints(31) . However, this assay does not measure frequency or fidelity of recombination separately, and may miss the error-prone tendency of A-T cells because the assay detects only functional rearrangements.

Error-prone Recombination

It has been suggested previously that A-T exhibits DNA mis-repair (32) which has been supported by plasmid-based repair assays(15, 16) . Our previous work using integrating plasmid repair probes, investigating the rejoining of cleaved plasmid, had suggested that the rejoin fidelity was dependent on the process of integration rather than extra-chromosomal religation. When rejoin errors were made by A-T cells, they were frequently duplicated in multiple copies in a single clone, but not reproduced between clones. This suggested that mis-repair may be recombination dependent. Two separate observations in this paper have supported the hypothesis of error-prone recombination. First, the development of hygromycin resistance after pTPSN transfection was mediated by complex sequence rearrangements in A-T compared with the sequence conserving mechanisms seen in normal cells. Second, the introduction of DNA cleavage into the gpt gene of pDelta2 and pDelta3 led to a reduction in accurate recombination in A-T, but enhanced recombination in normal cells.

A-T Gene Function

The relationship of error-prone recombination to the function of the A-T gene is unclear. The recombination events described may be many steps downstream from the direct function of the gene. If each time a DNA double-strand break occurs, multiple sequence errors are introduced, radiation sensitivity may result. Error-prone, hyper-recombination is consistent with being cancer prone. The immune defect is less clear: the V(D)J recombination assay is reported to be normal in A-T cells (31) but there is a clear increase in chromosomal aberrations triggered by V(D)J induced cleavage (33) . This mechanism may account for the increase in lymphoid malignancies in A-T. A lack of cell cycle checkpoint arrest may contribute to errors in recombination, but the link between cell cycle control and recombination in relation to the function of the A-T gene remains unclear. An impairment of damage surveillance or chromatin accessibility have been suggested to explain the link, but with little supportive data at present.


FOOTNOTES

*
This research was supported by United States Public Health Service Grant CA58985 from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
To whom correspondence should be addressed: Dept. of Radiation Oncology, Cox 302, 100 Blossom St., Massachusetts General Hospital, Boston, MA 02114. Tel.: 617-726-8669; Fax: 617-726-3603; :powell{at}hadron.mgh.harvard.edu.

(^1)
The abbreviations used are: A-T, ataxia telangiectasia; kb, kilobase(s); NF, normal fibroblast.


ACKNOWLEDGEMENTS

We thank Michael Liskay for use of the pTPSN plasmid, and John Thacker for the pDelta2/pDelta3 plasmids.


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