©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
The Cell Cycle in Polyploid Megakaryocytes Is Associated with Reduced Activity of Cyclin B1-dependent Cdc2 Kinase (*)

(Received for publication, June 13, 1995; and in revised form, November 29, 1995)

Ying Zhang Zhengyu Wang Katya Ravid (§)

From the Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

The platelet precursor, the megakaryocyte, matures to a polyploid cell as a result of DNA replication in the absence of mitosis (endomitosis). The factors controlling endomitosis are accessible to analysis in our megakaryocytic cell line, MegT, generated by targeted expression of temperature-sensitive simian virus 40 large T antigen to megakaryocytes of transgenic mice. We aimed to define whether endomitosis consists of a continuous phase of DNA synthesis (S) or of S phases interrupted by gaps. Analysis of the cell cycle in MegT cells revealed that, upon inactivation of large T antigen, the cells shifted from a mitotic cell cycle to an endomitotic cell cycle consisting of S/Gap phases. The level of the G(1)/S cyclin, cyclin A, as well as of the G(1) phase cyclin, cyclin D3, were elevated at the onset of DNA synthesis, either in MegT cells undergoing a mitotic cell cycle or during endomitosis. In contrast, the level of the mitotic cyclin, cyclin B1, cycled in cells displaying a mitotic cell cycle while not detectable during endomitosis. Comparable levels of the mitotic kinase protein, Cdc2, were detected during the mitotic cell cycle or during endomitosis; however, cyclin B1-dependent Cdc2 kinase activity was largely abolished in the polyploid cells. Fibroblasts immortalized with the same heat-labile oncogene do not display reduced levels of cyclin B1 upon shifting to high temperature nor do they become polyploid, indicating that reduced levels of cyclin B1 is a property of megakaryocytes and not of the T-antigen mutant. We conclude that cellular programming during endoreduplication in megakaryocytes is associated with reduced levels of cyclin B1.


INTRODUCTION

The development of hematopoietic cells consists of few stages: the commitment of pluripotent stem cells to differentiate rather than to remain in the resting G(0) phase or to proliferate, lineage restriction and maturation of these committed cells, and synthesis of cell-specific gene products. Although the mechanisms by which cells withdraw from the stem cell pool are presently unknown, it was suggested that the initial steps may be stochastic (Suda et al., 1983). Thereafter, growth factors support progenitor cells to develop along particular differentiation pathways (Metcalf, 1989). In the megakaryocytic lineage, endomitosis involving DNA synthesis in the absence of mitosis, as well as platelet production, are stimulated by thrombopoietin, recently isolated (Kaushansky et al., 1994; Wendling et al., 1994; Kuter et al., 1994; de Sauvage et al., 1994; Chang et al., 1995). The regulation of the cell cycle and of endomitosis in this cell type has not been explored yet.

The major events common to all eukaryotic cell cycles are the replication of chromosomes during S phase and their segregation during mitosis. The dependences of S phase and mitosis on each other ensure orderly progression through the cell cycle (Hartwell and Weinert, 1989). However, in some developmental situations, chromosome replication and segregation can be uncoupled. For example, a G(1)/S cycle takes place without any intervening mitosis in cells during the early development of the Drosophila embryo (Smith and Orr-Weaver, 1991), as may also be the case in ploidizing megakaryocytes. However, in most cells, the dependence of S phase upon mitosis (M) and of M on S phase are strictly observed. The regulation points of the cell cycle are the G(1)/S and G(2)/M transitions, for which the kinase activity of Cdk2 or Cdc2, respectively, is crucial. These kinases are controlled through association with regulatory subunits known as cyclins (Nasmyth, 1990; Evans et al., 1983; Westendorf et al., 1990; Pines and Hunter, 1990). The G(2)/M transition is dependent on the activity of the Cdc2 kinase, activation of which requires association of Cdc2 with B-type cyclins and dephosphorylation on tyrosine (Draetta et al., 1989; King et al., 1994). Regulators of the G(1)/S transition include the cyclin B1-dependent Cdc2 kinase (or its homologue Cdc28 in Saccharomyces cerevisiae) associated to cyclin A (Pines and Hunter, 1990; Bartlett and Nurse, 1990). In clams, these two different cyclins, A and B, show a similar periodicity in their synthesis and degradation, but in mammalian cells the levels of cyclin A rise near the beginning of S phase and the levels of cyclin B peak at the entry to mitosis (Evans et al., 1983; Westendorf et al., 1990; Pines and Hunter, 1990). The regulation of the G(1) phase was studied first in S. cerevisiae, where it was found that cyclins, cln1, cln2, and cln3 (CLN genes), control progression through G(1), by modulating the activity of Cdc28 kinase (Hadwiger et al., 1989; Wittenberg et al., 1990). Subsequently, equivalents to these G(1) cyclins have been identified, cyclins D1, D2, and D3 (reviewed by Reed, 1991).

Past studies of megakaryocyte development, endomitosis, and maturation have been hampered because of the rarity of megakaryocytes in bone marrow and because of the lack of a pure megakaryocytic cell line that can enter and complete a normal maturation cycle. Different leukemia cell lines derived from hematopoietic progenitors have been used in studying the biochemistry as well as gene regulation of the megakaryocytic lineage. Some human erythroleukemic cell lines exhibit myeloid, erythroid, as well as megakaryocytic markers (Martin and Papayannopoulou, 1982; Tabilio et al., 1983; Ravid et al., 1993a), while other cell lines (Greenberg et al., 1988; Sledge et al., 1986; Adachi et al., 1991) are enriched with megakaryocytic markers, but require exposure to a substance such as phorbol 12-myristate 13-acetate in order to ploidize. This later agent, being pluripotent, may induce changes in cyclin expression unrelated to megakaryocyte ploidy, as demonstrated on a nonmegakaryocytic cell line HL60 (Akiyma et al., 1993). We have recently generated a megakaryocytic cell line, MegT, by targeted expression of the temperature-sensitive form of large T antigen in megakaryocytes of transgenic mice, via the platelet factor four tissue-specific promoter (Ravid et al., 1993b). MegT cells which become polyploid upon inactivation of the oncogene were used to determine the role of different cyclins in promoting endomitosis. Our data suggest that once large T antigen is degraded, the cells undergo endomitosis while containing low levels of cyclin B1 and low activity of the mitotic kinase.


MATERIALS AND METHODS

Culture Conditions

MegT cells (clone 37C1) were grown in a liquid culture, all as described before (Ravid et al., 1993b). To induce ploidy, 1 times 10^6 cells were seeded into a 75-cm^2 culture flask and incubated in 5% CO(2) at 39.5 °C for 4-5 days (Ravid et al., 1993b). Cells were counted by hemocytometer, and cell viability was followed by staining with Trypan Blue. For synchronization experiments, cells were cultured for 24 h at the indicated temperature after which the cells were shifted to a medium containing 0.1% horse serum. Forty eight hours later, fresh medium containing 20% horse serum was added to the cells. The attached cells as well as the detached cells were collected separately at different time points and subjected to various analyses. Immortal human fibroblasts transformed by heat-labile (tsA58) T antigen (generous gift of Dr. Harvey Ozer) were cultured as described elsewhere (Hubbard-Smith et al., 1992).

Immunoprecipitation and Western Blotting

Immunoprecipitation and Western blot analyses were performed essentially as described before (Xiong et al., 1992). To this end, MegT cells adhering to the culture dish and nonadhering cells were collected separately by trypsinization or by spinning down cells (380 times g, 5 min) in the medium, respectively. Cells were washed twice with cold phosphate-buffered saline (PBS) (^1)(136 mM NaCl, 8 mM Na(2)HPO(4), 2.6 mM KCl, 1.4 mM KH(2)PO(4), pH 7.4) by centrifugation and lysed in lysis buffer (0.5% Nonidet P-40, 50 mM Tris, pH 7.4, 250 mM NaCl, 5 mM EDTA, 1 mM phenylmethylsulfonyl fluoride, 10 µg/ml N-tosyl-L-phenylalanine chloromethyl ketone, 10 µg/ml soybean trypsin inhibitor) followed by centrifugation at 15,000 times g for 5 min. Lysates, each containing 100 µg of protein in 400 µl of lysis buffer, were precleared by incubation with normal mouse IgG and 40 µl of Zysorbin (fixed and killed Staphylococcus aureus protein A, Zymed Laboratories, Inc., San Francisco, CA) for 1 h at 4 °C followed by centrifugation at 15,000 times g for 5 min. Antibody was added to the clarified lysate and incubated for 1 h at 4 °C. Protein A-Sepharose CL-4B (Pharmacia Biotech Inc.) was added at a volume of 40 µl and incubated at 4 °C for an additional hour. Immunoprecipitates were washed three times with lysis buffer, resuspended in sodium dodecyl sulfate (SDS) sample buffer, and separated on 12% SDS-polyacrylamide gel (SDS-PAGE) (Laemmli and Favre, 1973). Protein assays were done as recommended by the manufacturer (Bio-Rad Laboratories).

For Western blotting, 10 µg of lysed proteins were separated on 7.5% or 12% SDS-PAGE (Laemmli and Favre, 1973) and electrophoretically transferred from the gel onto an Immobilon-P membrane (Millipore) in a buffer containing 25 mM Tris, 192 mM glycine, and 20% methanol. The membrane was washed in TBS (10 mM Tris, pH 8.0, 150 mM NaCl) and blocked for 1 h with TBST (TBS with 0.05% Tween 20) containing 5% dry milk. The blot was washed four times for 5 min with TBST and incubated for 1 h at 4 °C in the presence of 10 ml of TBST supplemented with one of the following primary antibodies raised against human proteins: rabbit polyclonal antibodies to cyclin A (diluted 1:2000) (a gift of Dr. Tony Hunter at Salk Institute), anti-cyclin B1 monoclonal antibody (GNS-1 diluted to 0.2 µg/ml), anti-Cdc2 monoclonal antibody (A17 diluted to 0.5 µg/ml), anti-SV40 large T antigen monoclonal antibody (PAb 101 diluted to 1 µg/ml) (all from Pharmingen, San Diego, CA), rat antibody to human cyclin D3 (diluted to 0.2 µg/ml) (Oncoge Science, Uniondale, NY). All these antibodies cross-react with the corresponding mouse proteins, as tested by the manufacturers. The blot was washed four times, each time for 10 min, and incubated for 1 h with horseradish peroxidase-labeled appropriate secondary antibody (1:1500 to 1:3000 dilution in TBST (Amersham). The blot was washed four times, each for 10 min, with TBST and the Enhanced Chemiluminescence system (Amersham) was used for detection of proteins, as instructed by the manufacturer.

Kinase Assays

Kinase assays were done essentially as described before (Koff et al., 1991). Immunoprecipitates containing the GNS-1 antibody (see above), which do not block the binding of Cdc2 to cyclin B, and protein A-Sepharose CL-4B were washed in kinase buffer (50 mM Tris, pH 7.4, 10 mM MgCl(2), 1 mM dithiothreitol). The beads were resuspended in 50 µl of kinase buffer containing 2.5 µg of histone H1 (Boehringer Mannheim, Germany), 5 µCi of [-P]ATP (3000 Ci/mmol, DuPont NEN), 30 µM ATP (Sigma). After a 20-min incubation at 25 °C, an equal volume of 2 times SDS sample buffer was added. The sample was boiled for 5 min and centrifuged at 15,000 times g for 5 min. 20 µl of the sample was loaded on 12% SDS-PAGE (Laemmli and Favre, 1973). The gel was dried and subjected to autoradiography.

DNA Synthesis

MegT cells were seeded in a 25-cm flask at a concentration of 0.5 times 10^6 cells/5 ml of medium (Ravid et al., 1993b). The cells were either cultured at 34 °C or at 39.5 °C, as indicated. Cells were efficiently synchronized by serum starvation, as described above, and, at different time points after addition of serum, they were subjected to pulse labeling with [^3H]thymidine (15 µCi/5 ml) (DuPont NEN) for 1 h. At the end of the pulse labeling, the medium was collected, and the cells in suspension were spun down (380 times g, 5 min) and washed twice with PBS. The adhering cells also were washed with PBS. The adhering cells were extracted on the dish, and the nonadhering cells were extracted in a tube, both with 1.5 ml of 5% trichloroacetic acid for 30 min at room temperature. Trichloroacetic acid was discarded, and the cells were washed with an additional 2 ml of trichloroacetic acid. Cells were dissolved in 0.5 ml of 1 N NaOH which was then collected to a scintillation vial containing 5 ml of scintillation mixture (Fisher Scientific) and counted in a scintillation counter.

Flowcytometer Analysis

Cells were scraped off the plate with PBS or collected in medium, when in suspension, and pelleted for 5 min at 380 times g. Cells washed with PBS were resuspended in propidium iodide (0.05 µg/ml in 0.1% sodium citrate, pH 7.4) essentially as described before (Ravid et al., 1993b). Uniform nuclear staining was achieved over 16 h at 4 °C. Just before flow cytometry analysis, the cells were treated with ribonuclease at a final concentration of 0.05 mg/ml, for 30 min at room temperature. Cells were filtered through a 100-µm mesh, diluted with propidium iodide solution to a concentration of 10^6 cells/ml, and subjected to flow cytometry analysis on a FACScan system (Becton Dickinson). Data were collected and analyzed by CellFit program (Becton Dickinson, San Jose, CA).

Northern Blot Analysis

RNA isolation and Northern blot analyses were performed as described before (Ravid et al., 1993b). The blots were probed with different human cDNAs encoding different cyclins, each recognizing the corresponding mouse cDNA (a gift of Dr. Emma Lees, Massachusetts General Hospital Cancer Center), or with mouse cDNA generated by the polymerase chain reaction using DNA primers based on published sequences (Chapman and Wolgemuth, 1992). The blots were washed under low stringency for 60 min at 59 °C with 2 times SSC (Ravid et al., 1993b). Blots subjected to repeated hybridizations were first stripped by immersing the blot for 10 min in a preboiled solution of 0.1% SDS (w/v in H(2)O). Probing with actin cDNA was performed last to confirm equal loading of RNA in each lane.


RESULTS

Expression of Messages for Different Cyclins in MegT Cells

Proliferating MegT cells were synchronized by serum deprivation, and RNA was prepared from cells harvested at different time points of the cell cycle. The RNA was subjected to Northern blot analysis using different cyclin cDNAs as probes. As shown in Fig. 1, MegT cells expressed cyclins A, B1, D1, and D3 mRNAs, but to a lesser extent B2 mRNA, and not D2 mRNA. It should be pointed out that the B2 and D2 cDNAs used as probes detected the corresponding mRNA from mouse total bone marrow, confirming that the probes used were capable of recognizing the murine messages (not shown). Indeed, as described for other systems, B-type and D-type cyclins are differentially expressed in different tissues (Chapman and Wolgemuth, 1992, 1993; Matsushime et al., 1991).


Figure 1: Determination of mRNA for different cyclins in MegT cells. MegT cells were synchronized by serum starvation at 34 °C. At zero time and at successive time points after serum addition, a portion of the cells was subjected to nuclei staining and flow cytometry analysis (Ravid et al., 1993b) to determine the cell cycle point using the CellFit program, as described under ``Materials and Methods.'' A full cell cycle was completed within 24 h. RNA prepared from synchronized MegT cells at different time points (hours) was subjected to Northern blot analysis. Total RNA (15 µg/lane) was electrophoresed on 1% agarose gel which was then blotted onto nitrocellulose. The blot was probed with different cDNAs encoding different cyclins as well as with -actin cDNA to confirm equal loading of RNA. X-ray films exposed to the blots were developed after 48-h exposure, except for the ones probed with actin and large T antigen cDNAs which were exposed for 5 h. Two splicing forms of cyclin B1 (1.6 kb and 2.5 kb) and two of cyclin A (about 2.2 kb and 3.6 kb) were detected, as also described before (Chapman and Wolgemuth, 1992; Samejima and Yanagida, 1994).



Correlation between the Level of Large T Antigen and of the Mitotic Cyclin in Diploid and Polyploid MegT Cells

We noted that once MegT cells were shifted to the temperature which is not permissive for stability of large T antigen, a fraction of the cells remained adhering to the dish while the other fraction detached from the dish. The nonadhering cells, representing 34 + 9% (n = 6) of the cells at 4 days postculturing, appeared as round and larger cells. At the permissive temperature (34 °C), all cells remained adhering to the culture dish (Fig. 2). In the current study, we performed separate ploidy analyses on the detached and adhering cells cultured at the same nonpermissive temperature as compared to MegT cells cultured at 34 °C. Under the later conditions, all cells were adhering to the dish and consisted of 2N (diploid) and 4N cells (Fig. 3). At the nonpermissive temperature, the fraction of cells attached to the plate also consisted of 2N and 4N cells (Fig. 3B). In contrast, the majority of the detached cells were 4N and 8N cells (Fig. 3C). In a previous study, we found that the megakaryocyte-promoting factor, thrombopoietin (Kuter et al., 1994), did not have a significant effect on the ploidy state of MegT cells. (^2)Recently, these cells were also analyzed for their ability to express c-mpl, the receptor for thrombopoietin (Wendling et al., 1994). We found that the level of expression of c-mpl in MegT cells was low, revealed only by the polymerase chain reaction, but not by Northern blot analysis (not shown). This indicated that signaling pathways critical for reaching a ploidy state higher than 8-16N are not active in these cells.


Figure 2: Phase-contrast photomicrographs of MegT cells. MegT cells were cultured at 34 °C (A) or at 39.6 °C (B and C) for 4 days. The cells adhering to the dish (B) and the detached cells (C) in suspension were collected separately and viewed by phase-contrast microscope at a magnification of times 100.




Figure 3: Ploidy analysis of MegT cells. MegT cells were cultured at 34 °C or at 39.5 °C for 4 days. The cells adhering to the dish and the detached cells in suspension were collected separately and subjected to nuclei staining and flow cytometry analysis as described under ``Materials and Methods.'' The abscissa shows the DNA content on a logarithmic scale, determined based on fluorescence due to propidium iodide staining, and the ordinate reflects the number of cells at each DNA value (linear).



Of most interest was the observation that the level of large T antigen, as revealed by Western blot analysis, was notably reduced in the cells in suspension (polyploid cells) but not in the adhering cells (mostly diploid cells) cultured at the same nonpermissive temperature (Fig. 4A). We then determined the level of components of the mitosis promoting factor (MPF), being cyclin B and Cdc2, in both fractions of cells. As shown in Fig. 4A, the level of cyclin B1 protein, but not of Cdc2, was significantly reduced in the polyploid cells. Northern blot analysis indicated, however, that the level of cyclin B1 mRNA was not changed significantly in polyploid cells (Fig. 4B). In order to establish whether a reduced level of cyclin B is a property of a T-antigen mutant cell line or rather a property of polyploid megakaryocytes, we determined the level of T antigen and of cyclin B in immortal fibroblasts (AR5) transformed by origin-defective SV40 encoding a heat-labile T antigen (Hubbard-Smith et al., 1992). At high temperature, these cells display a reduced growth rate, but no hyperploidy (Resnick-Silverman et al., 1991). Western blot analyses (Fig. 5) indicated that, although T antigen was degraded in AR5 cells incubated at high temperature, the level of cyclin B was not altered.


Figure 4: The levels of cyclin B1 and Cdc2 in MegT cells. A, Western blot analysis of MegT cells cultured at 39.6 °C (lanes 1 and 2) or at 34 °C (lanes 3). Blots, loaded in each lane with 10 µg of protein prepared from adhering cells (lanes 2 and 3) or detached cells (lane 1), were reacted with antibodies to large T antigen or to Cdc2 or to cyclin B1 proteins. Equal loading of protein in each lane was confirmed by brief staining of the blot with 0.1% Ponceau S (not shown) followed by destaining prior to reacting with the indicated antibody. Large T antigen, cyclin B1, and Cdc2 appeared with the molecular masses of 82, 55, and 34 kDa, respectively, on the blot. These results are representative of four experiments performed. B, Northern blot analysis of MegT cells cultured at 34 °C or at 39.5 °C and collected as described in A. RNA concentration was determined by absorbance at 260 nm before loading on the agarose gel. Equal loading was confirmed by ethidium bromide staining of the ribosomal bands 28 S (5 kb) and 18 S (2.5kb) shown in the figure. C, the blot was probed with cDNA encoding mouse cyclin B1. Two splicing forms of cyclin B1 were detected (1.6 kb and 2.5 kb), as also described before (Chapman and Wolgemuth, 1992).




Figure 5: The levels of cyclin B1, Cdc2, and T antigen in immortal fibroblasts. A, Western blot analysis of AR5 cells (fibroblasts transformed by heat-labile tsA58 T antigen) cultured at 34 °C (lane 1) or at 39.5 °C (lane 2). Blots, loaded in each lane with 10 µg of protein, were reacted with antibodies to large T antigen or to Cdc2 or to cyclin B1 proteins. Equal loading of protein in each lane was confirmed by brief staining of the blot with 0.1% Ponceau S (not shown) followed by destaining prior to reacting with the indicated antibody. These results are representative of two experiments performed.



The Activity of the Mitotic Kinase during the Cell Cycle in Synchronized MegT Cells

Pulse labeling with radiolabeled thymidine was used to determine the profile of DNA synthesis in MegT cells, synchronized by serum deprivation and cultured at the nonpermissive temperature. Upon release from synchronization, at successive time points, the adhering and nonadhering cells were labeled for 1 h with [^3H]thymidine and collected separately. During 40 h in culture, two cycles of DNA synthesis were observed in MegT cells adhering to the dish, each spanning about 18 h. The value of [^3H]thymidine incorporation at the first peak of DNA synthesis was lower than the peak value during the second cycle, and the cell number doubled at the end of the second cycle, all as expected during a mitotic cell cycle (Fig. 6A). In contrast, each cycle of DNA synthesis in the detached cells was completed within 10 h with a short gap between the S phases (Fig. 6B). The cell number remained constant at the end of both cycles, as expected during endoreduplication. The second peak of DNA synthesis reached a value for [^3H]thymidine incorporation lower than the one expected in case all cells would have undergone endoreduplication. It should be pointed out, however, that also in the case of primary bone marrow megakaryocytes only a fraction of the cells continues endomitosis for several cycles to reach a high ploidy state (Rovolic, 1974). Immunoprecipitation of equal amounts of proteins prepared from the mitotic and endoreduplicating MegT cells revealed that the activity of the mitosis promoting factor (MPF), consisting of cyclin B-dependent Cdc2 kinase, was high during mitosis in the replicating MegT cells while hardly detectable in the polyploid cells (inset in Fig. 6).


Figure 6: DNA synthesis and mitotic kinase activity in synchronized MegT cells. Synchronized MegT cells were subjected at different time points to pulse labeling for 1 h with radiolabeled thymidine and were then collected for determination of incorporation of thymidine into DNA in the adhering (A) mitotic, and detached (B) endomitotic cells cultured at 39.5 °C. The inset shows histone phosphorylation by the mitotic kinase isolated at the indicated points of the cell cycle, all as described under ``Materials and Methods.'' Results are from a representative experiment, out of three performed.



The Level of Different Cyclins in Synchronized MegT Cells

We also sought to determine the level of G(1) phase, G(1)/S, and M phase cyclins at the onset and offset of DNA synthesis in synchronized MegT cells cultured at the nonpermissive temperature. As shown in Fig. 7A, Western blot analyses revealed that during the mitotic cell cycle the level of the G(1)/S cyclin, cyclin A, was slightly higher at the onset of DNA synthesis while elevation of cyclin B1 level started at S phase, as expected before entry to mitosis. During the endomitotic cell cycle (Fig. 7B), the levels of cyclin A and of the protein Cdc2 were significantly high, but cyclin B1 was barely detectable. As to the large T antigen, it was readily detectable during the mitotic cell cycle, but, undetectable in the polyploid cells. These results further suggested that turning off the oncogene was a prerequisite for shifting from a mitotic cell cycle to endomitosis. It should be also pointed out that, during the mitotic cell cycle, the level of cyclin A cycled moderately, while it did not seem to cycle during the endomitotic cell cycle. It is possible that we were unable to detect a rapid transient decrease in cyclin A during the short G phase of the endomitotic cell cycle. The level of the G(1) phase protein, cyclin D3, for which a high level of mRNA was detected (Fig. 1) was also determined. Cyclin D3 level was high at time points corresponding to the G(1) phase (Fig. 6) during the mitotic cell cycle while moderate changes were observed in the level of this cyclin during endomitosis (Fig. 8).


Figure 7: The levels of different cyclins in synchronized MegT cells. Cell lysates were prepared separately from adhering (A) mitotic and detached (B) endomitotic MegT cells, cultured at 39.5 °C, at different hours postrelease from synchronization. The cell lysates were subjected to Western blot analysis, using 12% acrylamide gel. Equal loading of protein prepared from endomitotic cells in suspension (C) and adhering mitotic (D) cells (10 µg of protein/lane) was confirmed by brief staining (1-2 min) of the blots with 0.1% Ponceau S in 5% acetic acid followed by destaining in water for 2 min and photography, followed by a 10-min rinse in water prior to reaction with the indicated antibodies. Lane M contains a ladder of molecular mass markers. Large T antigen, cyclin A, cyclin B1, and Cdc2 appeared with the molecular masses of 82, 60, 55, and 34 kDa, respectively, on the blot.




Figure 8: Cyclin D3 levels in MegT cells. Cell lysates were prepared separately from adhering (A) mitotic, and detached (B) endomitotic MegT cells, cultured at 39.5 °C, at different hours postrelease from synchronization. The cell lysates were subjected to Western blot analysis, using 10% acrylamide gel. Equal loading of protein prepared from endomitotic cells in suspension (C) and adhering mitotic (D) cells (10 µg of protein/lane) was confirmed by brief staining (1-2 min) of the blots with 0.1% Ponceau S in 5% acetic acid followed by destaining in water for 2 min and photography, followed by a 10-min rinse in water prior to reaction with cyclin D3 antibody. Cyclin D3 appeared with a molecular mass of 33 kDa on the blot.




DISCUSSION

Endomitosis, involving DNA replication in the absence of mitosis, can occur in three types of cells: those in which the endoreplicated chromosomes are not synapsed or visible, those in which cyclic condensation of the chromosomes is observed, and, in some cases, those in which multinucleate cells have been referred to as polyploid also. In the case of the megakaryocytic lineage, the endoreduplicated DNA is all concentrated in one nucleus (Metcalf, 1989). However, it is not certain yet if polyploid megakaryocytes enter only prophase involving chromosome condensation or prophase as well as metaphase, involving chromosome condensation and spindle formation but skip anaphase, or whether the cells skip all stages in the G(2) and M phases to directly enter a G(1) phase. While electron microscopic analyses of different stages of mitosis in megakaryocytes are underway, we sought to investigate the cyclin composition of megakaryocytes undergoing endomitosis.

In a previous study, we generated several clones of megakaryocytic cell lines by targeted expression of the temperature-sensitive form of large T antigen in transgenic mice, via the tissue-specific platelet factor four (PF4) promoter (Ravid et al., 1993b). These cell lines express several megakaryocytic markers, such as the glycoprotein GPIIb, and acetylcholine esterase, and at the permissive temperature adhere to the dish and contain high levels of large T antigen (Ravid et al., 1993b). In the current study, we have chosen to investigate a clone which allowed us to perform analyses of the mitotic and endomitotic cell cycle at the same nonpermissive temperature. When MegT cells (clone 37C1) were cultured at the nonpermissive temperature, only a fraction of the cells (about 30%) had undetectable levels of large T antigen and solely those cells appeared as round cells in suspension with high ploidy nuclei. Because of the leaky nature of this conditional oncogene, large T antigen may not have been completely destroyed in the rest of the cells which remained adhering to the dish. Nevertheless, this feature was taken as an experimental advantage, since it allowed us to compare levels of different cyclins in diploid and polyploid megakaryocytes cultured at the same elevated temperature. Our results indicated that cells expressing high levels of large T antigen were unable to initiate endoreduplication. In a recently published study, additional transgenic mice carrying the temperature-sensitive large T antigen under the control of the PF4 promoter have been generated (Robinson et al., 1994). Upon aging, some of these mice developed megakaryocytic leukemias displaying aberrations in megakaryocyte morphology and low platelet counts. Transgenic mice in which megakaryocytes reached high ploidy level seemed to express minute amounts of the oncogene (Robinson et al., 1994).

We aimed to define whether the megakaryocytic cell cycle during endomitosis consists of a continuous S phase or of Gap/S phases and to determine which cyclins are involved in this process. Pulse-labeling of MegT cells with [^3H]thymidine revealed that during endomitosis DNA synthesis was not continuous, but, rather, interrupted by a short gap. The level of the G(1) phase cyclin, cyclin D3, cycled during the mitotic cell cycle in MegT cells and rose at the gap phase during endomitosis. This latter result further suggested the existence of a G(1) phase during endomitosis in megakaryocytes. During this process of endoreduplication, the whole cell cycle was quite short, spanning about 10 h, in correlation with a previous study in rat primary bone marrow megakaryocytes (Odell et al., 1968). Further cyclin analyses in MegT cells revealed no significant differences in the level of cyclin A and in the level of Cdc2 protein during a mitotic or endomitotic cell cycle. However, while the cyclin B1 level rose at the onset of mitosis in continuously doubling MegT cells, it was hardly detectable in the polyploid cells. In accordance, the activity of cyclin B1-dependent kinase was low. Interestingly, the reduced level of cyclin B1 could not be attributed to low level transcription, as the level of cyclin B1 mRNA was not reduced in polyploid cells. The mechanism of cyclin degradation, a highly selective process, is not well understood. The amino-terminal sequences have been shown to play a critical role in targeting cyclins to the ubiquitin degradation pathway (Hershko et al., 1991), the activation of which occurs at the onset of anaphase. It is plausible then that cyclin B1 is either not stable or not translated in polyploid megakaryocytes. We confirmed that a reduced level of cyclin B1 is a property of polyploid megakaryocytes, rather than a property of our particular T-antigen cell line, by analyzing immortal fibroblasts transformed with the temperature-sensitive T antigen. At the nonpermissive temperature, these latter cells are not polyploid (Resnick-Silverman et al., 1991) and display a reduced level of T antigen, but not of cyclin B1. In accordance with these results is our observation that primary megakaryocytes derived from mouse bone marrow lack cyclin B1, as revealed by lack of staining (via immunohistochemistry) with cyclin B1 antibody (not shown).

Although two types of cyclin B were described in mammalian cells, B1 and B2 (Chapman and Wolgemuth, 1993), it is not clear yet which of these cyclins plays a role in different stages of mitosis in eukaryotic cells and if they are able to substitute for each other. While the level of cyclin B1 protein was reduced during endomitosis in MegT cells, we were unable to determine the level of cyclin B2 protein because of the lack of an antibody that recognizes mouse cyclin B2. It should be pointed out, however, that cyclin B1 mRNA is the predominant one in MegT cells (Fig. 1). Nevertheless, we do not exclude the possibility that small amounts of B2 mRNA may lead to a significant amount of B2 protein in MegT cells. In many systems tested, the lack of cyclin B1 alone is sufficient to drive endoreduplication. Endoreduplication in some Drosophila cell types is indeed associated with a lack of cyclin B1 (Lehner and O'Farrell, 1990). Also, the metaphase II arrest in mouse oocytes is controlled through destruction of cyclin B1 (Kubiak et al., 1993). Initiation of endoreduplication in different systems depends on the availability of Cdc2 kinase and cyclin B, both composing an active M phase promoting factor (MPF). Thus, certain treatments, such as inhibitors of protein kinases in mammalian cells or high levels of the protein encoded by rum1, which inhibits the mitotic kinase in fission yeast, block M phase and induce repeats of S phase (Usui et al., 1991; Moreno and Nurse, 1994). In the filamentous fungus Aspergillus nidulans, the NIMA protein kinase is required in addition to MPF for the M phase (O'Connell et al., 1994). Overexpression of this kinase in different systems, including human cells, resulted in chromatin condensation without other aspects of mitosis (O'Connell et al., 1994). Although functional homologues of NIMA in mammalian cells have not been described yet, some kinases have been reported to have homology to the catalytic domain of NIMA (Schultz and Nigg, 1993). These studies suggest that not all steps of mitosis are regulated by the MPF kinase. The fission yeast Schizosaccharomyces cerevisiae temperature sensitivity cut8-563 mutation causes chromosome overcondensation and short spindle formation in the absence of cytokinesis, thus leading to the identification of a gene (cek1) encoding a novel protein kinase which complements the mutation that blocks anaphase (Samejima and Yanagida, 1994). The model proposed by O'Connell et al.(1994) involves condensation of chromatin by a NIMA homologue while other mitotic events down to anaphase are regulated by MPF. Anaphase is regulated by inactivation of MPF and may be regulated also by other kinases such as cek1 analogues. If so, our model of polyploid MegT cells with significantly reduced levels of cyclin B1 may be used for studying the roles of different mitosis-related kinases in endomitosis.


FOOTNOTES

*
This work was supported in part by NHLBI National Institutes of Health Grant HL53080-02 (to K. R.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
Established Investigator of the American Heart Association. To whom correspondence should be addressed: Biochemistry K225, Boston University School of Medicine, 80 East Concord St., Boston, MA 02118. Tel.: 617-638-5053; Fax: 617-638-5054.

(^1)
The abbreviations used are: PBS, phosphate-buffered saline; PAGE, polyacrylamide gel electrophoresis; MPF, mitosis promoting factor; kb, kilobase(s).

(^2)
K. Ravid, D. Kuter, and R. Rosenberg, unpublished data.


ACKNOWLEDGEMENTS

We thank Tony Hunter and Emma Lees for valuable insight, Dimitry Kamen for helpful technical assistance, and Harvey Ozer for the generous gift of immortal SVtsA/HF-A cells.


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