©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
Insulin-like Growth Factor Expression in Human Cancer Cell Lines (*)

(Received for publication, September 28, 1995; and in revised form, March 1, 1996)

Kathryn A. Quinn (§) Anthony M. Treston Edward J. Unsworth Mae-Jean Miller Michele Vos Chris Grimley (1) James Battey (2) James L. Mulshine Frank Cuttitta

From the  (1)Biomarkers and Prevention Research Branch, NCI, National Institutes of Health, Rockville, Maryland 20850, the Applied Biosystems Division, Perkin-Elmer Corp., Foster City, California 94404, and the (2)NIDCD, National Institutes of Health, Gaithersburg, Maryland 20850

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

The insulin-like growth factors (IGFs), IGF-I and IGF-II, are potent mitogens for human lung and other epithelial cancer cell lines. Previous studies in defined medium lacking added IGF or insulin suggest that an IGF-related ligand can act as an autocrine growth factor for many cancer cell lines through action via the type I IGF receptor (IGF-R). Analysis of RNA isolated from human lung and breast cancer cell lines by reverse transcription of mRNA and polymerase chain reaction reveal that IGF-I and IGF-II mRNAs were co-expressed with IGF-R in the majority of cell lines. IGF-I mRNA was detected in 11/12 small cell lung cancer cell lines (SCLC), 13/14 non-small cell lung cancer (NSCLC) cell lines, and 1/2 breast cancer cell lines. IGF-II mRNA was detected in 8/10 SCLC, 11/12 NSCLC cell lines, and 2/2 breast lines. All cell lines expressed IGF-R. For analysis of IGF peptide secretion, cell lines were adapted to growth in serum/hormone-free culture medium (R(0)), and to avoid interference by IGF-binding proteins, secreted IGF peptides were isolated under acidic conditions and analyzed by Western blotting. Based upon measurement of the sensitivity of the anti-IGF antibodies for detection of recombinant human IGFs, IGF peptides accumulated in conditioned medium at greater than picomolar concentrations should have been readily detected. In three cell lines (two lung and one breast) secreted IGF immunoreactivity was detected as three molecular mass species of 23, 14, and 6 kDa. Isolation and NH(2)-terminal sequencing of each of these species definitively identified them as differentially processed forms of the IGF-II prohormone. Despite the high frequency of IGF-I gene expression detected by reverse transcription-polymerase chain reaction analysis, only one lung cancer cell line, NCI-N417d, was found that unequivocally secreted IGF-I peptide. This direct sequence determination unambiguously identifies IGF-II as the predominant IGF involved in the autocrine growth stimulation of human lung and breast epithelial tumor cell lines and supports a growing body of literature that implicates IGF-II/IGF-R autocrine loops as a common growth mechanism in epithelial carcinogenesis.


INTRODUCTION

Recent studies have identified the type I insulin-like growth factor receptor (IGF-R) (^1)as a potential control point for transformed cells(1, 2, 3) . Using a variety of approaches, it has been demonstrated that the growth and tumorigenicity of transformed cells can be inhibited by the perturbation of IGF-R function(1, 4, 5) , and IGF-R has been implicated in the protection of tumor cells from apoptosis(3) . Human epithelial cancer cell lines express the IGF-R(6, 7, 8, 9) , proliferate in response to the IGFs(4, 6, 8) , and exhibit reduced rates of growth when cultured in the presence of the monoclonal antibody alpha-IR3, which inhibits the interaction of the IGF-R with its ligands(4, 8, 10) . The ligands that bind IGF-R with high affinity are the insulin-like growth factors I (IGF-I) and II (IGF-II). These studies show that in vitro in defined medium that lacks IGFs or insulin, an IGF-related ligand can function as an autocrine growth factor for human epithelial cancer cell lines. Therefore, autocrine or paracrine loops involving the IGF-R and its ligand/s may be crucial determinants for the in vivo growth and tumorigenicity of transformed epithelial cells.

To date, few definitive data have characterized the synthesis and secretion of IGFs from human cancer cell lines. Most studies carried out on the mitogenic and autocrine effects of the IGFs have utilized exogenous addition of IGF-I(4, 8) . For lung cancer cells, this approach was initially supported by work from several groups who reported small (milliunits/milliliter) amounts of ``IGF-I-like immunoreactivity'' in conditioned growth medium from lung cancer cell lines(11, 12) . However, these original studies are now recognized to be confused by IGF-binding proteins (IGFBP), by IGF-II cross-reactivity with the antibodies used for the IGF-I measurements(13) , and have been contradicted by later analysis of a small number of lung cancer cell lines from one of these groups(11) , which suggests low and infrequent expression of IGF-I mRNA and a higher level of IGF-II mRNA expression. Comparing the data in these and other papers, it is apparent that IGF-R is frequently expressed(7, 14) , but the identity of the relevant ligand is still unclear; the IGF-I-like immunoreactivity is not IGF-I (cell lines positive for immunoreactivity are negative for mRNA expression by RT-PCR), and there is post-transcriptional regulation of synthesis and/or secretion of the IGFs (cell lines positive by RT-PCR and Northern analysis are negative for secreted immunoreactivity)(11, 12, 15) .

Elucidation of the identity of the critical IGF-R ligand is necessary for potential early tumor detection and therapeutic interventions in a wide range of human malignancies. In order to determine the identity of the secreted IGF involved in cancer cell autocrine growth, we have carried out an extensive characterization of the expression of IGF mRNA, and the synthesis, processing, and release of IGF peptides by human lung and breast epithelial cancer cell lines. For these studies we used both Northern analysis and reverse transcription of mRNA followed by PCR (RT-PCR) to examine gene expression. We also purified to homogeneity several differentially processed and glycosylated IGF peptides secreted by human cancer cell lines, and we have definitively identified the peptides by amino-terminal Edman sequence analysis.


MATERIALS AND METHODS

Cell Lines

There are six main histologies of lung cancer: the neuroendocrine small cell lung cancers (SCLC) and the non-SCLC (NSCLC), which comprise squamous, adenocarcinoma, bronchoalveolar carcinoma, carcinoid, and large cell tumors. For these studies we analyzed 14 SCLC of classic and variant subtypes, 12 NSCLC cell lines, and 2 breast cancer cell lines obtained from the NCI-Navy Medical Oncology Branch, Bethesda, MD (listed in Table 1). Reagents for cell culture, unless otherwise indicated, were obtained from Life Technologies, Inc. Cells were cultured at 37 °C in an atmosphere of 5% CO(2) in RPMI 1640 with 5% heat-inactivated fetal bovine serum, or under serum- and hormone-free conditions (R(0) medium: phenol red-free RPMI 1640 with 5 mML-glutamine and 5 times 10M sodium selenite (Sigma)). Cells and conditioned medium (R(0)CM) were harvested for analysis after the cells were adapted to the R(0) medium and proliferated independently of serum components. Once adapted, the cell lines continue to grow in R(0) medium and can undergo multiple passages(16) .



Analysis of IGF Gene Expression

Cells from one 175-cm^2 tissue culture flask (approximately 5 times 10^6 to 10^7 cells) were washed twice with ice-cold Dulbecco's phosphate-buffered saline, and poly(A) RNA was isolated directly from cells using a Micro-Fast Track mRNA isolation kit (Invitrogen Corp.), according to the manufacturer's instructions. IGF gene expression was initially analyzed by Northern blot, carried out according to standard procedures (17) . For RT-PCR, poly(A) RNA (1 µg) was reverse-transcribed using a SuperScript(TM) kit (Life Technologies, Inc.), according to the manufacturer's instructions.

The single IGF-I gene comprises five exons, which are alternatively spliced to generate two mRNA transcripts(18) . The two translation products, the IGF-IA and IGF-IB prohormones, differ in the region that encodes the carboxyl-terminal extension peptide (E-peptide), which is cleaved from pro-IGF-I during biosynthesis(18) . Reactions for amplification of IGF-I cDNA were primed with an oligonucleotide complementary to the common mRNA region, which encodes the IGF-I NH(2) terminus, and antisense primers were complementary to sequences in the IGF-IA and IGF-IB splice variants. The primers had the following sequences: IGF-I sense, 5`-GGACCGGAGACGCTCTGCGG-3`; IGF-IA antisense, 5`-TCTACTTGCGTTCTTCAAAT-3`; IGF-IB antisense, 5`-TTTGCCTCTGCATTCAGCAT-3`. The IGF-II gene encodes a single prepro-IGF-II, from which the mature 67 amino acid IGF-II is cleaved during biosynthesis(19) . Primers for the amplification of IGF-II cDNA sequences were as follows: IGF-II sense, 5`-AGTCGATGCTGGTGCTTCTCA-3`; IGF-II antisense, 5`-GTGGGCGGGGTCTTGGGTGGGTAG-3`.

The integrity of the RNA and the efficiency of RT-PCR were monitored by amplifying the cDNA with primers specific for human type I IGF-R, which is ubiquitously expressed by human lung cancer cell lines. The primers for amplification of IGF-R were as follows: IGF-R sense, 5`-ATTGAGGAGGTCACAGAGAAC-3`; IGF-R antisense, 5`-TTCATATCCTGTTTTGGCCTG-3`. PCR reaction mixes consisted of 2 µl of cDNA, 100 ng each of 3` and 5` primer, 2.5 units of Taq polymerase (Ampli-Taq, Applied Biosystems Division, Perkin-Elmer) in 100 µl of PCR buffer (1.5 mM MgCl(2), 200 µM dNTPs, 50 mM KCl, 10 mM Tris-HCl, pH 8.3). For amplification of IGF-I sequences, 35 cycles of PCR was programmed as follows: 94 °C, 15 s/50 °C, 15 s/72 °C, 15 s; followed by a final 5-min extension at 72 °C. For PCR with IGF-II and IGF-R primers, the annealing temperature was raised to 60 °C.

Gels were Southern blotted onto 0.2-µm nitrocellulose (Schleicher & Schull) in 20 times SSC, baked at 80 °C for 2 h, and hybridized overnight at 42 °C with 10^6 cpm/ml oligonucleotide probe complementary to sequences found between the PCR primers. The sequence of the antisense oligonucleotide probes were as follows: IGF-IA probe, 5`GCGCTCGGCACGGACAGAGCG; IGF-IB probe, 5`-TCCAATCTCCCTCCTCTGCT-3`; IGF-II probe, 5`AGGCGCTGGGTGGACTGC-3`; IGF-R probe, 5`-GTACTCTGTCTCCAGCTCTTC-3`. The probe was end-labeled with [-P]ATP using T4 polynucleotide kinase (Life Technologies, Inc.) and diluted in hybridization buffer-N(17) . After hybridization the blots were washed at room temperature twice in 2 times SSC and once in 0.5 times SSC. Autoradiography was performed at -70 °C on Kodak X-AR film with an intensifying screen. PCR product derived from cell line H1385 was cloned into the TA cloning vector (Invitrogen, San Diego, CA) and sequenced using the dsCycle sequencing kit (Life Technologies, Inc.) according to the manufacturer's instructions.

Anti-IGF Antibodies

For immunoblot studies investigating the secretion of IGF peptides from cultured cell lines, a commercial monoclonal anti-IGF-I (Upstate Biotechnology, Inc., Lake Placid, NY), reported to show 50% cross-reactivity with IGF-II, was used. An IGF-I-specific antibody was raised by immunizing rabbits with a peptide antigen comprising amino acids 26-41 of the mature IGF-I peptide. The peptide was amidated at the carboxyl terminus to enhance stability in vivo, and a tyrosine residue was included to allow iodination of the synthetic peptide. The sequence of the peptide IGF was H(2)N-YNKPTGYGSSSRRAPQT-CONH(2). Of this sequence only three amino acids (Ser-Arg) are shared with IGF-II. Sera from rabbits immunized with keyhole limpet hemocyanin-coupled peptide and the anti-IGF mAb were evaluated for specificity of IGF-I and IGF-II detection by Western immunoblot.

Immunoblot Analysis

Samples were separated electrophoretically on a 10-20% polyacrylamide gel (Novex, San Diego, CA) using the Tricine buffer system(20) , electroblotted onto PVDF membrane (Millipore-Waters, Bedford, MA), blots were blocked at 37 °C for 2 h in 1% (w/v) bovine serum albumin in phosphate-buffered saline and incubated overnight at 4 °C in 1 µg/ml anti-IGF monoclonal antibody or 1/1000 dilution rabbit anti-IGF. For detection of bound antibody with I-protein A, blots probed with anti-IGF mAb were incubated with a secondary antibody (1/1000 rabbit anti-mouse antibody; DakoPatts, Glostrup, Denmark) for 1 h at room temperature. Washed blots were incubated with 10^6 cpm I-protein A (1 h, room temperature), washed, and autoradiographed. The specificity of the immunodetection was established by presaturating the diluted antibodies with 1 µM rhIGF. For quantitation, blots were scanned using a PhosphorImager (Molecular Dynamics, Sunnyvale, CA).

Analysis of Cell Lines for IGF Peptides

Initially, both whole cell lysates and conditioned medium from cultured cell lines were screened for IGFs by immunoblot analysis. Whole cell lysates were prepared by lysing 10^6 cells directly in SDS-PAGE sample buffer (without mercaptoethanol or bromphenol blue). Lysates were clarified by centrifugation at 100,000 g, and protein was estimated using the BCA assay (Pierce). Up to 100 µg of total cellular protein was electrophoresed for immunoblot assay of intracellular IGFs. For analysis of IGF peptides secreted into R(0)CM, the CM was collected and IGF peptides were concentrated by HPLC.

HPLC Fractionation of Conditioned Medium

Cell lines were initially screened for the secretion of IGF peptides by collection of 7-day conditioned medium from cells grown with R(0) medium. The R(0)CM was clarified by centrifugation at 3000 times g at 4 °C and stored at -20 °C in the presence of the protease inhibitors bacitracin (1 µM; Bachem, Torrance, CA), phosphoramidon (1 µM, Sigma), and Pefabloc (1 mM) added to the R(0)CM after collection. Five hundred milliliters of R(0)CM was adjusted to pH 2.5 with trifluoroacetic acid (Pierce), mixed at 4 °C overnight, centrifuged at 4000 times g for 30 min, and filtered through a 0.45 µM filter. The R(0)CM was adjusted to 10% (v/v) acetonitrile and pumped at 15 ml/min onto a C(4) reverse phase column (30 mm times 30 cm DeltaPak 300R, Millipore-Waters) equilibrated in 10% acetonitrile, 0.1% trifluoroacetic acid (v/v). The column was washed with 10% acetonitrile, 0.1% trifluoroacetic acid (v/v), and a biphasic gradient was applied with acetonitrile increased from 10% to 35% (v/v) over 15 min and to 45% (v/v) over the next 30 min. Fractions (0.48 min; 12 ml) were collected, freeze-dried and resuspended in 200 µl of SDS-PAGE sample buffer. Before each R(0)CM was chromatographed, a blank gradient was run and screened for residual immunoreactivity from the previous run. IGF-I and IGF-II standards were chromatographed under identical conditions after all the R(0)CM samples were processed.

Stability of IGF-I in CM

R(0)CM (500 ml) from the cell line NCI-H2087 was supplemented with 5 µg of rhIGF-I (final concentration 1.2 nM) and fractionated by C(4) HPLC as described above. Fractions were collected, freeze-dried, and analyzed by immunoblot for the presence of IGF-I and IGF-II. The IGF-positive fractions were pooled and treated in an identical fashion for the purification and NH(2)-terminal sequencing of the IGF peptides (see below) as those isolated from unsupplemented R(0)CM.

Purification and NH(2)-terminal Sequencing of Secreted IGFs

For isolation of anti-IGF immunoreactivity, R(0)CM (up to 10 liters) was fractionated by preparative scale HPLC on a C(4) column. Fractions positive for IGF immunoreactivity were applied to a phenyl reverse-phase HPLC column (5 µ; 2.1 mm times 25 cm, Vydac, The Separations Group, Hesperia, CA) equilibrated with 20% acetonitrile, 0.1% heptafluorobutyric acid (v/v) (HFBA, Pierce), and eluted with a gradient to 45% acetonitrile, 0.1% HFBA (v/v) over 150 min at 1 ml/min. One-minute fractions were collected. For amino acid sequencing, fractions containing the 6- and 14-kDa immunoreactive species were freeze-dried and resuspended in 50 µl of SDS-PAGE buffer and separated on 15-cm 16% Tricine-PAGE gels(20) . On the 16% gel, the IGF standards migrated close to 7.5 kDa. The fractions containing the 23-kDa immunoreactive IGF species were rechromatographed on an analytical C(4) column with a gradient from 10% acetonitrile, 0.1% trifluoroacetic acid (v/v) to 50% acetonitrile, 0.1% trifluoroacetic acid (v/v) over 60 min. Thirty-second (0.5 ml) fractions were collected. The positive fractions were electrophoresed on a 15-cm 12% Tricine SDS-PAGE gel. Samples for sequencing were electroblotted onto PVDF in 10 mM CAPS, pH 11, containing 10% (v/v) methanol for 2 h at 100 V at 4 °C. The blots were stained with 0.125% Coomassie Blue R250, 10% acetic acid, 50% methanol (w/v/v). Bands of the appropriate electrophoretic mobility were excised and sequenced by automated Edman degradation on an Applied Biosystems model 494 Procise Sequencer (Applied Biosystems Division, Perkin-Elmer).


RESULTS

IGF mRNA Is Widely Expressed in Human Cancer Cell Lines

Both IGF-I and IGF-II were co-expressed by the majority of cell lines tested, and all cell lines expressed mRNA for the IGF-R (summarized in Table 1). Corroborating an earlier report on a small number of cell lines, the level of IGF-II mRNA in some lung cancer cell lines appeared higher than that of IGF-I(10) , i.e. IGF-I mRNA was not detectable by Northern blot for any cell line. However, IGF-II mRNA was detectable for three of the 23 lung cancer cell lines (NCI-H820, -H69C, and -H2087) and for the breast cancer cell line H2380 (data not shown). The pattern of IGF and IGF-R gene expression by RT-PCR in a subset of the cancer cell lines is shown in Fig. 1(A and B). The majority of lung cancer cell lines (21/26) co-expressed both the IGF-IA and IGF-IB splice variants. Both breast cancer cell lines H2380 and MCF-7 expressed IGF-II, whereas MCF-7 was negative for both IGF-I mRNA forms and H2380 expressed both variants (Fig. 1B). After this current study was completed, a third IGF-I splice variant, also differing in the region that encodes the E-peptide, was described (21) . To date we have not characterized its expression in these human cancer cell lines.


Figure 1: IGF and IGF-R gene expression in lung cancer cell lines as detected by RT-PCR (A) and IGF and IGF-R gene expression in the breast cancer cell lines (B). RT-PCR products were electrophoresed on agarose gels, Southern blotted, and probed with specific oligonucleotide probes complementary to an internal sequence to confirm the identity of the PCR products. The faster migrating band of the doublet visible with the IGF-IB primers is single-stranded PCR product; when excised and reamplified, it yielded a product that migrated with the upper band. The figure shows a subset of the data used to generate Table 1. Some bands not visible in the photograph were seen on longer exposure of the probed Southern blot to film.



IGF Gene Expression Shows Small Changes after Adaptation to Growth under R(0) Conditions

There was no change in the overall pattern of IGF gene expression when cells were adapted to growth under R(0) conditions, with the exception of three cell lines, NCI-H720, -H1092, and -H146, which began to express low levels of the IGF-IB splice variant. Although the pattern of IGF gene expression by the cell lines appeared to be largely unchanged after adaptation to R(0) conditions, there may be quantitative differences in the mRNA levels that were not detected using the current techniques.

IGF Peptides Are Secreted into CM

The sensitivity and specificity of the anti-IGF antibodies used to screen Western blots is shown in Fig. 2. No specific IGF immunoreactivity was detected in whole cell lysates of the cancer cell lines with either of the anti-IGF antibodies. As the commercial anti-IGF mAb proved to be sensitive for the detection of both IGF-I and -II, this antibody was used for the initial screening of HPLC-fractionated R(0)CM.


Figure 2: Specificity of anti-IGF antibodies for the detection of recombinant human IGF-II and IGF-I. Varying amounts of the rhIGF standards as indicated were electrophoresed on 10-20% Tricine SDS-PAGE gels and Western blotted. Blots were incubated with either the anti-IGF mAb or the polyclonal anti-IGF.



In the initial screening of 500 ml of R(0)CM, immunoreactivity was detected in medium conditioned by only 3/10 cell lines: NCI-H820, NCI-H2087, and NCI-H2380. Fig. 3A shows the chromatogram obtained when R(0)CM from the cell line NCI-H2087 was fractionated on a C(4) column. IGF immunoreactivity (Fig. 3B) was in present as three bands of 23, 14, and 6-kDa and eluted in fractions 34-36. When IGF-I and IGF-II standards were chromatographed on the preparative C(4) column, they had the same retention time as the immunoreactivity from R(0)CM and they co-migrated with M(r) 6000 species on the mini-gels used for the immunoblotting (data not shown). The relative intensity and distribution of the three immunoreactive species varied between these cell lines (Fig. 3C). Electrophoresis under reducing conditions did not alter the apparent size of the three immunoreactive species, suggesting that they are monomeric species.


Figure 3: Western blot analysis of R(0)CM concentrated by RP-HPLC using anti-IGF mAb. A, chromatograph of 500 ml of H2087 R(0)CM separated on C(4) RP-HPLC; B, Western blot analysis of fractions from chromatography of H2087 R(0)CM analyzed with anti-IGF monoclonal antibody; C, IGF immunoreactivity in fractionated R(0)CM from lung and breast cancer cell lines (500 ml each). Western blots were probed with the anti-IGF mAb, and relative amounts of each immunoreactive species were quantitated using a PhosphorImager. &cjs2112;, 23-kDa form; &cjs2113;, 14-kDa form; , 6-kDa form.



When larger volumes of R(0)CM (3-7.5 liters) from the cell lines NCI-H345 and NCI-N417 were concentrated on the C(4) column, weak immunoreactive bands corresponding to M(r) 6000 were detected after extended exposure of Western blots (data not shown). R(0)CM from the cell lines NCI-H187 (2 liters), NCI-H1385 (1.5 liters), NCI-H510 (3 liters), MCF-7 (2 liters), and NCI-H460 (3 liters) tested negative for IGF immunoreactivity.

Specificity of Immunoreactivity

The specificity of the immunoreactivity detected by the IGF mAb in concentrated R(0)CM is shown in Fig. 4(top panel). When the anti-IGF mAb was preincubated with 1 µM IGF-I, the immunoreactive material was no longer recognized, indicating that it is specific and IGF-related (Fig. 4, lanes B). The polyclonal anti-IGF-I did not detect any immunoreactive material in the NCI-H2087 R(0)CM shown or in R(0)CM from any of the other cell lines investigated (Fig. 4, top panel, lane C). This antibody is highly specific for rhIGF-I and did not recognize 100 ng IGF-II on Western blot (Fig. 2, lower panel), although it is approximately as sensitive as the less specific commercial mAb for IGF-I. The M(r) 6000 immunoreactive species co-migrates with rhIGF on SDS-PAGE and presumably represents mature IGF. The failure of the anti-IGF-I to recognize this band suggested that it was IGF-II. The larger molecular size immunoreactive species presumably represent unprocessed or partially processed IGF prohormones. The ability of the anti-IGF-I to recognize incompletely processed IGF-I prohormone is not known, and the failure of this antibody to recognize the 14- and 23-kDa immunoreactive species did not eliminate the possibility that these forms were unprocessed or partially processed IGF-I prohormones.


Figure 4: Specificity of immunoreactivity detected by Western blotting. Top panel, H2087 R(0)CM was concentrated by RP-HPLC as in Fig. 3A, and samples of the peak immunoreactive fraction were Western blotted under the conditions shown. Bottom panel, H2087 R(0)CM with 5 µg of rhIGF-I added (final concentration 1.2 nM) was concentrated by RP-HPLC as for the top panel. Lane a, probed with anti-IGF mAb (1 µg/ml); lane b, probed with anti-IGF mAb (1 µg/ml) + 1 µM rhIGF-I; lane c, probed with 1/1000 polyclonal anti-IGF-I.



Stability of IGF-I in R(0)CM

The failure of the anti-IGF-I antibody to detect fully processed 7.5-kDa IGF-I in the fractionated R(0)CM samples raised the possibility that it may have become degraded or was inefficiently recovered by the isolation procedures. To address this concern, rhIGF-I was added in a physiologically relevant concentration (2.5 µg/liter; 1.2 nM) into H2087 R(0)CM after collection, stored as usual, and fractionated on the C(4) column in a process identical to the other R(0)CMs. The second panel of Fig. 4demonstrates that the exogenous IGF-I was recovered and could be detected by the anti-IGF anti-serum. We also used Edman degradation to confirm that IGF-I sequence, at the level of 10 pmol, could be obtained from 1/20 of the pooled positive fractions purified by HPLC from NCI-H2087 R(0)CM into which 5 µg (660 pmol) of rhIGF-I had been added. This represents 30% recovery of the exogenously added rhIGF-I, determined from the initial and repetitive sequencer yield. However, rhIGF standards subjected to SDS-PAGE, electroblotted onto PVDF, and quantitated by NH(2)-terminal sequencing also resulted in a ``recovery'' of 25-50%. Given this yield at the blotting/sequencing step, 30% recovery of rhIGF-I from H2087 R(0)CM after storage and chromatographic isolation procedures followed by electroblotting and sequencing demonstrates that IGF-I is stable in R(0)CM and would have been recovered with minimal losses under the purification conditions used for these studies.

Purification and NH(2)-terminal Sequencing of Secreted Immunoreactivity

Given that the antibody studies did not confirm the identity of the IGF immunoreactivity secreted by the R(0)-adapted cell lines, we conducted a large scale purification of R(0)CM from the cell lines NCI-H2087 (10 liters), NCI-H820 (2 liters), and NCI-N417 (7.5 liters), and the breast line, NCI-H2380 (500 ml). The elution profile of the pooled fractions 34-36 from the preparative C(4) column rechromatographed on a phenyl column with an acetonitrile/HFBA gradient is shown in Fig. 5. When standards were run on this column with the same gradient, IGF-II eluted at 73.4 min and IGF-I at 77.6 min. Ubiquitin, which had been identified as a contaminant in the IGF-positive preparative C(4) HPLC fractions by amino acid sequencing of parallel fractions from the IGF-negative cell line H187, eluted in a broad peak from 92 min to 105 min. Coomassie staining of fractions after SDS-PAGE and electroblotting onto PVDF revealed protein bands that corresponded with the IGF immunoreactivity. The results of Edman sequence analysis of these bands are listed in Table 2. All of the immunoreactive species secreted by the cell lines NCI-H820, NCI-H2087, and NCI-H2380 were identified as products of the IGF-II gene. IGF-I was identified by Edman sequence analysis of a peptide isolated from the variant SCLC line NCI-N417.


Figure 5: Separation of IGF immunoreactive species by RP-HPLC. A, phenyl RP-HPLC of pooled IGF-containing fractions from NCI-H2087 R(0)CM concentrate eluted with a gradient of acetonitrile in 0.1% HFBA; B, Western blot of fractions analyzed with the anti-IGF mAb.






DISCUSSION

Previous in vitro studies have suggested that an IGF/IGF-R autocrine loop may be operating in many human epithelial cancer cell lines. The inhibitory effect of the anti-IGF-R antibody, alpha-IR3, on the growth of lung cancer cell lines has been documented (4, 10) , and studies by others and ourselves have confirmed the inhibitory effect of this antibody on the clonal growth of the breast cancer cell line MCF-7 in semi-solid agar in the presence of serum (8) and the absence of added serum or insulin-like peptides. (^2)Our previous studies have shown that IGF stimulation of tumor cell growth is mediated via an active IGF-R(4) . Recent work has confirmed the importance of IGF/IGF-R interactions in maintenance of the transformed phenotype and control of apoptosis(1, 2, 3) . The capacity of the lung and breast cancer cell lines investigated in these studies to adapt to growth in unsupplemented basal medium with minimal changes in the pattern of IGF gene expression suggests that IGF/IGF-R autocrine loops may be constitutive elements which influence the growth characteristics of these tumors. As IGF-R is expressed by most actively growing cells, elucidation of the identity of the synthesized and secreted IGF ligand is crucial to our understanding of epithelial tumor biology.

The two mature 7.5-kDa IGF peptides exhibit close to 70% amino acid identity with each other and are 50% homologous with pro-insulin(22) . Studies have implicated both IGFs in the growth and differentiation of normal epithelia. In the developing fetal lung, IGF-II appears to play a critical role, since mice lacking a functional IGF-II gene died at birth due to a failure of lung inflation(5) . In situ hybridization studies have localized IGF-I expression to the mesenchymal compartment of adult and developing lung, suggesting that IGF-I may influence airway development via paracrine modes of action (23) . Corroborating evidence has demonstrated the secretion of IGF-I by primary cultures of human lung fibroblasts(24) . These findings suggest that the pattern of IGF expression in lung neoplasms may parallel that of the breast epithelium, where IGF-I is principally expressed by stromal tissue while IGF-II is expressed by epithelial tumor cells (25) . Elucidation of the role of IGFs in autocrine growth is complicated by the synthesis of IGFBP which are expressed by many cancer cell lines, usually in large excess over the IGFs(14, 15, 26) . The IGFBP therefore confound interpretation of early studies, which aimed to use anti-IGF antibodies to investigate the effect of IGF neutralization on cell growth. Using purification and assay methodologies that obviate interference by IGFBP, we have demonstrated conclusively the secretion by human lung and breast cancer cell lines of ligand/s that can bind and activate the IGF-R.

Purification, SDS-PAGE, and NH(2)-terminal Edman sequencing demonstrated IGF-II peptide secretion in the breast adenocarcinoma cell line (H2380), and two of the lung cancer cell lines investigated: an adenocarcinoma (NCI-H2087) and a bronchoalveolar carcinoma (NCI-H820). IGF-I peptide sequence was obtained from an M(r) 6000 species isolated from medium conditioned by a variant SCLC cell line, NCI-N417. IGF-II was secreted as multiple molecular weight forms, as has been described for IGF-II present in normal adult serum and serum from hypoglycemic patients bearing mesenchymal tumors(27) . The post-translational processing of the primary IGF-II prepropeptide is complex and can yield multiple molecular weight forms of IGF-II, the nature of which is determined by differential post-translational cleavage and glycosylation events(19, 28) . The 20-kDa preproprotein is sequentially processed by cleavage at a single lysine residue at position 21 of the prohormone to yield a 10.5-kDa peptide (pro-IGF-II E21), which has been isolated from normal human serum(29) , and then by removal of the E-peptide to yield mature IGF-II. A 15-kDa form of pro-IGF-II E21 has also been isolated, which includes O-linked sialic acid residues attached to Thr^8 of the E-peptide(28) .

Studies are continuing to characterize fully the exact nature of the 14- and 23-kDa IGF-II species. The 6-kDa form identified here, which co-migrated on SDS-PAGE with the 7.5-kDa rhIGF-I/II standards, was identified as fully processed mature IGF-II. The primary IGF-II translation product has been calculated to be a 20-kDa polypeptide (30) . The 23-kDa form of IGF-II isolated here could represent the unprocessed precursor, which may be running with an artifactually high molecular mass on SDS-PAGE gels, or could be due to glycosylation at Thr^8 of the E-peptide region of the intact prepropeptide. Preliminary data indicates that the 14-kDa species, isolated from the H2087 adenocarcinoma, can be converted to a species migrating at 10 kDa on SDS-PAGE after incubation with O-glycanase and neuraminidase (data not shown). It is therefore equivalent to the 15-kDa IGF-II characterized by Hudgins et al.(28) . This glycosylated and sialated 15-kDa IGF-II has higher potency for growth stimulation and receptor activation than the 7.5-kDa peptide and altered interactions with IGFBP(27, 31) . At least two of the three forms of IGF-II isolated from these lung and breast tumor cell lines are therefore capable of acting as autocrine growth factors.

From the incidence of IGF gene expression shown in Table 1, it would appear that IGF autocrine loops are operating in the majority of the cell lines, i.e. IGF-R was co-expressed with one or both of the IGF peptide genes. IGF-II peptide secretion was detected in 3 of the 10 cancer cell lines intensively investigated. These three were cell lines with IGF-II mRNA levels detectable by the less sensitive Northern blot procedure. All of the cell lines investigated, with the exception of the SCLC cell line NCI-H187 and the breast line MCF-7, expressed one or both of the IGF-I transcripts. However, we were only able to definitively identify IGF-I peptide secretion by the SCLC line NCI-N417d. In the cell lines from which IGFs were not isolated, we calculate (based upon the sensitivity of the antibodies for the detection of the IGFs) that IGF peptides may only accumulate in CM at subpicomolar concentrations. As IGF-R inhibition studies with mAb alpha-IR3 demonstrate active IGF/IGF-R autocrine loops in many of the cell lines studied here(4) , mechanisms could be operating by which a low level of synthesized IGF is preferentially delivered to the receptor and rapidly turned over, resulting in accumulation of synthesized IGF at levels too low for measurement using present techniques. Such mechanisms may be potentiated by the IGFBP, which we found to be secreted by human lung cancer cell lines in large excess of the endogenously secreted IGFs. Membrane-bound forms of IGFBP, which have been identified in lung cancer cell lines(14) , may play a role in the delivery of IGFs to the receptor.

Addendum-Since this work was submitted for publication, a report on a single prostate cancer cell line has suggested that IGF-II/IGF-R is involved with autocrine growth regulation in that epithelial system(32) , and a recent study demonstrated that an IGF-II/IGF-R autocrine loop mediates epidermal growth factor-induced proliferation in a cervical cancer epithelial cell line(9) . Those findings, as well as the conclusive identification of multiple active forms of IGF-II synthesized and secreted by both lung and breast epithelial cancer cell lines reported here, point to the conservation of the IGF-II/IGF-R autocrine pathway as a central mechanism in the process of epithelial carcinogenesis.


FOOTNOTES

*
This work was supported by a generous grant from the G. Harold and Leila Y. Mathers Charitable Trust. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
Present address: Center for Thrombosis & Vascular Research, University of New South Wales, Kensington 2052, New South Wales, Australia.

(^1)
The abbreviations used are: IGF-R, Type I insulin-like growth factor receptor; CM, conditioned medium; IGF-I, insulin-like growth factor-I; IGF-II, insulin-like growth factor II; IGFBP, insulin-like growth factor-binding protein; SCLC, small cell lung cancer; NSCLC, non-small cell lung cancer; rhIGF, recombinant human IGF; mAb, monoclonal antibody; CAPS, 3-(cyclohexylamino)propanesulfonic acid; PCR, polymerase chain reaction; RT-PCR, reverse transcription PCR; PVDF, polyvinylidene difluoride; PAGE, polyacrylamide gel electrophoresis; HPLC, high performance liquid chromatography; HFBA, heptafluorobutyric acid; Tricine, N-tris(hydroxymethyl)methylglycine.

(^2)
K. A. Quinn, M.-J. Miller, and F. Cuttitta, unpublished observation.


ACKNOWLEDGEMENTS

We thank Renato Baserga for helpful discussions throughout this work.


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