©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
Phorbol Ester Regulation of Opioid Peptide Gene Expression in Myocardial Cells
ROLE OF NUCLEAR PROTEIN KINASE C (*)

(Received for publication, August 30, 1995)

Carlo Ventura (1)(§) Gianfranco Pintus (1) Isabella Vaona (3) Federico Bennardini (2) Giangavino Pinna (1) Bruna Tadolini (1)

From the  (1)Institute of Biological Chemistry ``A. Bonsignore'', School of Medicine, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, the (2)Institute of Biological Chemistry, School of Pharmacy, University of Sassari, Via Muroni 23/A, 07100 Sassari, and the (3)Department of Biochemistry, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

Opioid peptide gene expression was characterized in adult rat ventricular cardiac myocytes that had been cultured in the absence or the presence of phorbol 12-myristate 13-acetate. The phorbol ester induced a concentration- and time-dependent increase of prodynorphin mRNA, the maximal effect being reached after 4 h of treatment. The increase in mRNA expression was suppressed by incubation of cardiomyocytes with staurosporine, a putative protein kinase C inhibitor, and was not observed when the cells were cultured in the presence of the inactive phorbol ester 4alpha-phorbol 12,13-didecanoate. Incubation of cardiac myocytes with phorbol 12-myristate 13-acetate also elicited a specific and staurosporine-sensitive increase in immunoreactive dynorphin B, a biologically active end product of the precursor, both in the myocardial cells and in the culture medium. In vitro run-off transcription assays indicated that transcription of the prodynorphin gene was increased both in nuclei isolated from phorbol ester-treated myocytes and in nuclei isolated from control cells and then exposed to phorbol 12-myristate 13-acetate. No transcriptional effect was observed when cardiac myocytes or isolated nuclei where exposed to 4alpha-phorbol 12,13-didecanoate. The phorbol ester-induced increase in prodynorphin gene transcription was prevented by pretreatment of myocytes or isolated nuclei with staurosporine, suggesting that myocardial opioid gene expression may be regulated by nuclear protein kinase C. In this regard, cardiac myocytes expressed protein kinase C-alpha, -, -, and -, as shown by immunoblotting. Only protein kinase C- and protein kinase C- were expressed in nuclei that have been isolated from control myocytes, suggesting that these two isotypes of the enzyme may be part of the signal transduction pathway involved in the effect elicited by the phorbol ester on opioid gene transcription in isolated nuclei. The incubation of myocardial nuclei isolated from control cells in the presence of a protein kinase C activator induced the phosphorylation of the myristoylated alanine-rich protein kinase C substrate peptide, a specific fluorescent substrate of the enzyme. The possibility that prodynorphin gene expression may control the heart function through autocrine or paracrine mechanisms is discussed.


INTRODUCTION

Opioid peptides have been shown to have a wide range of tissue distribution and are known to control the cardiac function through reflex mechanisms involving the central nervous system or the modulation of neurotransmitter release from neurons located within the heart(1, 2) . The discovery that the mammalian myocardial cells possess opioid receptors (3) has led to studies aimed at investigating direct myocardial effects due to opioid receptor stimulation and identifying possible intracellular opioidergic pathways. In this regard, we have shown that, in adult rat ventricular cardiac myocytes, k opioid receptor stimulation depleted the sarcoplasmic reticulum of Ca and caused a marked reduction in the amplitudes of the cytosolic Ca transient and of the associated twitch(4) . In addition to affecting cytosolic Ca homeostasis, the incubation of cardiac myocytes with k opioid receptor agonists also elicited intracellular alkalosis and increased myofilament Ca responsiveness(5) , an event that blunted in part the negative inotropic effect due to the intracellular Ca depletion. However, the myocardial cell, besides being a target for opioid agonists, also acts as a source for opioid peptides. We have recently shown that the prodynorphin gene is expressed in adult cultured rat ventricular cardiac myocytes and that these cells are able to synthesize and release dynorphin B(6) , an opioid peptide that binds selectively to k opioid receptors(7) . Similar to the synthetic k opioid receptor agonist U-50,488H, dynorphin B was able to elicit Ca depletion from an intracellular storage site when it was acutely released over single cardiac myocytes(6) . These findings suggest that the myocardial function may be affected in an autocrine or paracrine fashion by an opioid gene and by intracellular pathways that may regulate its expression.

The signal transduction pathway(s) involved in the expression of the prodynorphin gene in cardiac myocytes is currently unknown. However, the observed effects of k opioid receptor agonists on myocardial cytosolic Ca and pH homeostasis were largely attributable to the capability of these opioids to increase the formation of inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetraphosphate (8) and to elicit a protein kinase C-dependent stimulation of the Na/H antiporter(5) , indicating that myocardial opioid receptors are coupled to phosphoinositide turnover and protein kinase C (PKC). (^1)This enzyme is expressed in various forms and is widely distributed in different tissues (9, 10, 11) . In the myocardial cell, PKC activation, besides representing an important step in the opioidergic pathway, has been reported to regulate the expression of different genes, including c-fos and skeletal alpha-actin genes(12) . PKC activation by phorbol esters has also been shown to induce the human proenkephalin gene transfected into CV 1 cells, and a DNA sequence on this opioid gene has been identified conferring the sensitivity to phorbol ester induction(13) . The role of protein kinase C in the regulation of prodynorphin gene expression in cells naturally expressing this gene and, in particular, in the myocardial cell, has not been investigated, however.

In the present study, we aimed at elucidating whether PKC activation induced by phorbol 12-myristate 13-acetate (PMA) may influence the expression of prodynorphin mRNA as well as the synthesis and release of dynorphin B in adult cultured rat ventricular myocytes. The effect induced by the phorbol ester on the transcription of the prodynorphin gene and the presence of a PKC in isolated myocardial nuclei were also investigated.


MATERIALS AND METHODS

Isolation of Cardiac Myocytes

Hearts from 2-3-month-old male Wistar rats were retrogradely perfused through the aorta in the presence of a bicarbonate buffer, pH 7.4, containing low calcium and collagenase(14) . Following the isolation procedure, the cardiomyocytes were subjected to a short term serum-free primary culture, according to a previously described method(15) . Briefly, the cardiac myocytes were cultured under 5% CO(2) atmosphere at 37 °C in Petri dishes (60-mm Falcon dishes, Becton Dickinson) at a density of 2 times 10^5 cells/dish in 3 ml of M-199 medium (with Earle's salts) containing 0.2% bovine serum albumin, 10M insulin, 2.5 times 10M penicillin G, 2.5 times 10M streptomycin, and 10M cytosine arabinofuranoside. One day after plating, cells were treated under the experimental conditions described in the legend of each figure.

RNA Extraction and Determination of Prodynorphin mRNA

Following RNA isolation from the adult cultured myocytes (16) , the levels of prodynorphin mRNA were determined by using a sensitive solution hybridization RNase protection assay(17, 18) . Briefly, a 400-base pair HindIII-BamHI fragment of the main exon of rat genomic prodynorphin clone was inserted into pGEM3. Transcription of the plasmid linearized with BamHI generated a sense strand of prodynorphin mRNA used to construct a standard curve of prodynorphin mRNA, whereas transcription of the plasmid linearized with EcoRI in the presence of [P]CTP (800 Ci/mmol) gave an antisense strand used to hybridize cellular prodynorphin mRNA. The protected fragments were recovered after phenol chloroform extraction and electrophoretically separated in a polyacrylamide nondenaturing gel. Autoradiographic exposure was performed for 48 h. The individual bands were counted for radioactivity by liquid scintillation spectrometry, and cpm values were translated to pg values on a correlated standard curve. Data were expressed as pg of mRNA/µg of total RNA.

Identification of Dynorphin B-like Material

Immunoreactive dynorphin B (ir-dyn B) was measured by a radioimmunoassay procedure that utilized the 13 S antiserum raised against dyn B and capable of recognizing the high molecular weight peptides cleaved from the prodynorphin precursor and containing dyn B in their sequence(19, 20) . Acetic acid extracts from cardiac myocytes or pooled samples from the culture medium were processed by reverse-phase high performance liquid chromatography. The collected fractions were radioimmunoassayed according to a previously described method(6, 21) , and the immunoreactivity was attributed to authentic dyn B by comparison with the elution position of a synthetic standard.

Isolation of Myocardial Nuclei

Nuclei from control or PMA-treated cells were isolated by a modification of a previously described method(22) . Cells were rinsed twice with a physiological saline solution (Buffer I) containing 137 mM NaCl, 1.2 mM MgSO(4), 5 mM KCl, 1.2 mM NaH(2)PO(4), 20 mM HEPES, 16 mMD-glucose, and 1 mM CaCl(2), pH 7.4. The dishes were scraped with Buffer I, and the cells from pooled samples were sedimented at 1000 times g for 10 min at 4 °C. The cardiac myocytes were then resuspended in a hypo-osmotic buffer (Buffer II) of the following composition: 10 mM Tris/HCl, pH 7.4, 1 mM MgCl(2), 10 mM NaCl, 5 mM CaCl(2), 0.1 mM dithiothreitol, 0.5 mM phenylmethylsulfonyl fluoride, 1 µM leupeptin, and 10 mM beta-mercaptoethanol. The resuspended cells were incubated for 30 min at 4 °C and then sedimented at 1000 times g for 10 min at 4 °C. The pellet was resuspended in 20 ml of Buffer II and sonicated for 40 s at setting 2 in a Branson sonifier W-350. The sonicated preparation was added with Triton X-100 at a final concentration of 0.1% and then centrifuged at 1000 times g for 10 min at 4 °C. The pellet was resuspended in a buffer (Buffer III) containing 10 mM Tris, pH 8.0, 0.3 M sucrose, 5 mM MgCl(2), 0.1 mM dithiothreitol, 0.5 mM phenylmethylsulfonyl fluoride, 1 µM leupeptin, and 10 mM beta-mercaptoethanol. The resuspended material was layered over an equal volume of Buffer III containing 0.6 M sucrose and centrifuged at 1500 times g for 10 min at 4 °C. The resulting nuclear pellet was resuspended in a buffer (Buffer IV) containing 50 mM Tris/HCl, pH 8.0, 5 mM MgCl(2), 0.1 mM EDTA, 40% glycerol, 0.1 mM dithiothreitol, 0.5 mM phenylmethylsulfonyl fluoride, 1 µM leupeptin, and 10 mM beta-mercaptoethanol.

Nuclear Run-off Transcription Assay

Nuclear run-off experiments were carried out by using a modification of the method described by Greenberg(23) . Nuclei resuspended in 90 µl of Buffer IV were added with 100 µl of 2 times reaction buffer (10 mM Tris/HCl, pH 7.5, 5 mM MgCl(2), 0.3 M KCl, 5 mM dithiothreitol, 1 mM each of ATP, GTP, and CTP), and 10 µl of [alpha-P]UTP (3000 Ci/mmol), followed by incubation at room temperature for 15 min. DNA was digested by incubating the transcription mixture for 5 min at room temperature in the presence of 1 µl of 20,000 units/ml RNase-free DNase. Nuclear RNA was isolated by using guanidine thiocyanate and acid phenol extraction (16) , followed by purification on RNAMATRIX (BIO 101, Inc. Vista, CA). The P-labeled RNA pellet was resuspended in 1 ml of TES solution containing 10 mM TES, pH 7.4, 10 mM EDTA, and 0.2% SDS. Samples were eventually diluted by adding TES solution to equalize the cpm of P-labeled RNA/ml (about 5 times 10^6). 1 ml of RNA solution was then mixed with 1 ml of TES/NaCl solution (TES solution containing 0.6 M NaCl). A total volume of 2 ml of RNA solution was then hybridized to 1 µg of heat-denatured DNA insert corresponding to a 400-base pair fragment of the main exon of rat genomic prodynorphin clone that has been immobilized on nitrocellulose filters following isolation from a pGEM3 plasmid. In this regard, it has been shown that the use of a DNA insert versus the whole plasmid-containing insert can considerably increase the efficiency of hybridization(17) .

The hybridization reaction was allowed to run for 36 h at 65 °C. After the hybridization, the filters were washed in 2 times SSC for 1 h at 65 °C with several changes and treated with RNase A for 30 min at 37 °C. The filters were washed again in 2 times SSC for 1 h at 37 °C and exposed to Kodak X-Omat film at -70 °C. The amount of prodynorphin gene transcript was quantitated by liquid scintillation counting. Denatured plB15, a cDNA clone encoding for rat cyclophilin(24) , was slot blotted onto nitrocellulose and used as a positive control. pBR322 plasmid DNA was also hybridized with the labeled RNA as a negative control.

Immunoblotting Analysis of Nuclear PKC

Samples were electrophoresed on 10% SDS-polyacrylamide gels and were then transferred to nitrocellulose membrane at 250 mA for 1 h according to Towbin et al.(25) . After transfer, the blot was saturated for 1 h at room temperature with 2% fat-free milk powder in PBST (80 mM Na(2)HPO(4), 20 mM NaH(2)PO(4), 100 mM NaCl containing 0.05% Tween 20, pH 7.5). The immunoreaction was carried out for 1 h at room temperature in PBST and 2% milk powder containing the primary antibody diluted 1:1000 (antisera to PKC-alpha, PKC-, PKC-, and PKC- were from Calbiochem). After being washed three times with PBST (5 min each wash), the blot was incubated for 1 h at room temperature in the presence of the secondary antibody (1:5000 horseradish peroxidase-linked donkey anti-rabbit immunoglobulin) in PBST containing 1% fat-free milk powder. Finally, the nitrocellulose membranes were washed three times in PBST, developed by the ECL method, and exposed to films for 5 min (the secondary antibody as well as the ECL Western blotting detection reagents and the autoradiography film Hyperfilm MP were all from Amersham International).

Measurement of Nuclear PKC Activity

PKC activity from isolated myocardial nuclei was measured by using a continuous fluorescence assay (26, 27) that utilized the acrylodan-labeled myristoylated alanine-rich protein kinase C substrate (MARCKS) peptide (Boehringer Mannheim), a high affinity substrate in vitro for PKC(28, 29, 30, 31) . The MARCKS peptide consists of a conserved sequence of 24 amino acids from the MARCKS protein, which includes four sites for PKC phosphorylation(27) . The fluorescing acrylodan label is introduced N-terminally via an additional cysteine group. In the presence of PKC activators, maximum fluorescence is measured at 480 nm with excitation at 370 nm. In the course of phosphorylation by PKC, the intensity of the fluorescence decreases about 20%(27) . In the present study, the fluorescence changes during phosphorylation of the MARCKS peptide were monitored at 25 °C. The reaction mixture contained, in a final volume of 1 ml, 10 mM Tris/HCl, pH 7.0, 90 mM KCl, 3 mM MgCl(2), 0.3 mM CaCl(2), 0.1 mM EGTA, 100 µM ATP, 10% ethylene glycol, 0.5 µg phosphatidylserine, 0.1 µg 1,2-dioctanoyl-sn-glycerol, and 75 nM acrylodan-labeled MARCKS peptide. Phosphorylation of the acrylodan-labeled peptide was started by adding 20 µg of nuclear protein.

Data Analysis

The statistical analysis of the data was performed by using the unpaired Student's t test, assuming a p value less than 0.05 as the limit of significance.


RESULTS

RNA extracted from adult cultured rat ventricular cardiac myocytes was subjected to a solution hybridization RNase protection analysis using a synthetic P-antisense probe for detection. Incubation of the cultured myocytes in the presence of 100 nM PMA induced a marked increase in the amount of detectable mRNA. This stimulatory effect was evident after 1 h of cell exposure to the phorbol ester and peaked at 4 h of treatment. (Fig. 1). On the contrary, incubation of the cardiac myocytes for 4 h in the presence of 100 nM 4alpha-phorbol 12,13-didecanoate, a biochemically inactive phorbol, failed to affect the expression of prodynorphin mRNA (Fig. 1). When the cardiac myocytes were pretreated for 30 min with 2 nM staurosporine, a putative PKC inhibitor(32) , and subsequently treated for 4 h with PMA in the presence of this PKC inhibitor, the levels of prodynorphin mRNA did not differ significantly from those observed in control cardiac myocytes (Fig. 1). After 6 h of treatment with PMA, the levels of prodynorphin mRNA progressively declined and resulted in being down-regulated to the control value between 12 and 24 h (Fig. 1). Fig. 2reports the dose-response curve of the effect of PMA on prodynorphin mRNA expression, following a 4-h exposure of the cultured myocytes to the phorbol ester. A significant increase in mRNA levels was observed at a concentration as low as 1 nM. The amount of prodynorphin mRNA was progressively increased in a dose-dependent manner by cell treatment in the presence of the phorbol ester, reaching a plateau when the myocytes where cultured in the presence of PMA concentrations ranging between 50 and 100 nM.


Figure 1: Time course of the effect of PMA on the expression of prodynorphin mRNA in adult cultured rat ventricular myocytes. White bars, control; black bars, 100 nM PMA; hatched bar, 2 nM staurosporine; black bar with white hatching, 100 nM PMA and 2 nM staurosporine; shaded bar, 100 nM 4alpha-phorbol 12,13-didecanoate. The data are expressed as the mean values ± S.E. (n = 6). *, significantly different from the control value. Representative autoradiograms of the ribonuclease protection analysis of myocardial prodynorphin mRNA are shown in the inset. A, control; B, 100 nM PMA for 1 h; C, 100 nM PMA for 4 h; D, 4 h of exposure to 100 nM PMA in the presence of 2 nM staurosporine; E, 4 h of incubation in the presence of 100 nM 4alpha-phorbol 12,13-didecanoate. Autoradiographic exposure was for 2 days on Kodak X-Omat film with an intensifying screen. The mark indicates the position of a 400 base pair radiolabeled DNA marker, showing that the single protected fragment migrates with a molecular size of 400 bases, corresponding to prodynorphin mRNA.




Figure 2: Dose-response of PMA effect on the expression of prodynorphin mRNA. The myocardial cells were incubated in the presence of each concentration of PMA for a period of 4 h. box, control; bullet, PMA. The data are expressed as the mean values ± S.E. (n = 6). *, significantly different from the control value. Representative autoradiograms of the ribonuclease protection analysis of myocardial prodynorphin mRNA are shown in the inset. Autoradiographic exposure was carried out as described in Fig. 1.



Consistent amounts of ir-dyn B were found under basal conditions in cardiac myocytes and in the culture medium (Fig. 3). Culturing of myocytes for 4 h in the presence of 100 nM PMA resulted in a marked increase in the level of this biologically active peptide product of the prodynorphin gene both in the cells and in the culture medium (Fig. 3). The same figure shows that the cellular levels of ir-dyn B were significantly lower than those detected in the medium, both in the absence and the presence of the phorbol ester. Myocyte treatment with 2 nM staurosporine suppressed the PMA-mediated increase in ir-dyn B both in the medium and in the myocardial cell (Fig. 3). No significant increase in ir-dyn B levels was observed in cells or medium when the myocytes were cultured for 4 h in the presence of 100 nM 4alpha-phorbol 12,13-didecanoate (Fig. 3).


Figure 3: Regulation of ir-dyn B levels by PMA. Adult cultured rat ventricular myocytes were treated for 4 h with 100 nM PMA in the absence or presence of 2 nM staurosporine (ST) or exposed for a period of 4 h to 100 nM 4alpha-phorbol 12,13-didecanoate (4alpha-phorbol). Shaded bars, ir-dyn B in cultured myocytes; white bars, ir-dyn B in the medium. Each single value in the medium was calculated in a final volume of 15 ml, corresponding to the volume of pooled samples of the culture medium from 10^6 cells. Each experiment was performed in the presence of a peptidase inhibitor mixture containing 20 µM bestatin, 1 mM leucyl-L-leucine, 3 µM poly-L-lysine, 0.3 µM thiorphan, 30 µM 1-10-phenanthroline, and 6 µM 1,4-dithiothreitol. The data are expressed as the mean values ± S.E. (n = 6). *, significantly different from its own control value; , significantly different from its own control value; , the value of the white bar is significantly different from that of the shaded bar.



To investigate whether the increase in prodynorphin mRNA expression elicited by PMA reflects changes in the transcriptional status of the myocyte nucleus, we assessed the rate of transcription of the proynorphin gene by using an in vitro run-off transcription assay. The nuclear preparation used in the present study lacked contamination by sarcoplasmic reticular membranes, inner or outer mitochondrial membranes, or sarcolemmal membranes, as indicated by the measurement of the activities of the corresponding marker enzymes rotenone-insensitive NADPH-cytochrome c reductase, succinate dehydrogenase, rotenone-insensitive NADH-cytochrome c reductase, and 5`-nucleotidase(33, 34, 35) , which were all undetectable in the nuclear fraction (data not shown). We found that incubation of the cardiac myocytes in the presence of 100 nM PMA, a concentration that proved to be the most effective in increasing prodynorphin mRNA, resulted in a marked increase in nuclear transcription of the prodynorphin gene (Fig. 4). Interestingly, in nuclei isolated from untreated cells and subsequently exposed for 4 h to 100 nM PMA, prodynorphin gene transcription was found to be enhanced at a level similar to that observed in the nuclei from PMA-treated cardiomyocytes (Fig. 4). The pretreatment of cardiomyocytes or isolated nuclei with staurosporine abolished the PMA-induced increase in transcriptional activity. No increase in transcription of the prodynorphin gene was observed following incubation of myocytes or isolated nuclei in the presence of 100 nM 4alpha-phorbol 12,13-didecanoate (Fig. 4).


Figure 4: Effect of PMA on the rate of transcription of the prodynorphin gene in isolated myocardial nuclei. Isolation of myocardial nuclei and run-off transcriptional analysis of the prodynorphin gene were performed as described under ``Materials and Methods.'' plB15, a cDNA encoding rat cyclophilin, and pBR322 plasmid DNA were included as positive and negative controls, respectively. The values are representative of six experiments. 1, transcription of the prodynorphin gene; 2, pBR322; 3, plB15. A, nuclei were isolated from control cardiac myocytes; B, nuclei were isolated from cardiac myocytes that have been exposed to 100 nM PMA for 4 h; C, nuclei were isolated from untreated myocytes and subsequently exposed to 100 nM PMA for 4 h; D, nuclei were isolated from cardiac myocytes cultured for 4 h in the presence of 100 nM PMA and 2 nM staurosporine; E, nuclei isolated from untreated cells were subsequently incubated for 4 h in the presence of 100 nM PMA and 2 nM staurosporine; F, nuclei were isolated from cardiac myocytes that have been exposed to 100 nM 4alpha-phorbol 12,13-didecanoate for 4 h; G, nuclei were isolated from untreated myocytes and then incubated in the presence of 100 nM 4alpha-phorbol 12,13-didecanoate for 4 h.



Immunoblot analyses of total extracts from untreated cardiac myocytes revealed the expression of PKC-alpha (80 kDa), PKC- (78 kDa), PKC- (97 kDa), and PKC- (75 kDa) (Fig. 5). PKC-beta and PKC- were not detected (not shown). Western blot analysis of nuclear samples from untreated cells revealed that only PKC- and PKC- were expressed in the myocardial nucleus (Fig. 5). The densitometric analysis of the bands corresponding to PKC- and PKC- indicated that each of these two PKC isotypes was almost completely expressed at nuclear level (not shown).


Figure 5: Immunoblot analysis of PKC isotypes in isolated myocardial nuclei or total cell lysates. Nuclei and total cell lysates were prepared from control myocytes and subjected to immunoblotting (50 µg of protein/lane) as described under ``Materials and Methods'' with antisera to PKC-alpha, PKC-, PKC-, and PKC-. The arrows to the left of each panel indicate PKC immunoreactivity as confirmed in peptide antigen competition experiments (results not shown). The numbers to the right of each panel refer to the molecular mass (kDa) of marker proteins. Lane 1, myocardial nuclei; lane 2, total cell lysates. The values are representative of four separate experiments.



We next investigated whether the exposure of isolated myocardial nuclei to a PKC activator may result in the phosphorylation of a specific PKC substrate. Fig. 6shows the effect induced on acrylodan-peptide phosphorylation by the incubation of myocardial nuclei, isolated from control cells, in the absence or the presence of 1,2-dioctanoyl-sn-glycerol, a diglyceride that acts as a potent PKC activator(36) . When the nuclear fraction was incubated with the acrylodan peptide in the absence of the PKC activator, no significant change in the peptide fluorescence was observed (Fig. 6). On the contrary, exposure of the myocardial nuclei to 1,2-dioctanoyl-sn-glycerol for 10 min resulted in a time-dependent fluorescence decrease at the 480 nm emission maximum of the PKC substrate, corresponding to PKC-mediated acrylodan-peptide phosphorylation (Fig. 6). The maximal decrease in the intensity of fluorescence was achieved about 10 min from the addition of each nuclear sample to the reaction mixture.


Figure 6: Effect induced on acrylodan-peptide fluorescence by exposure of isolated myocardial nuclei to a PKC activator. Myocardial nuclei isolated from control myocytes were incubated for 10 min in the absence (box) or the presence () of the PKC activator 1,2-dioctanoyl-sn-glycerol (0.1 µg/ml). Each nuclear sample was added at the time indicated by the arrow. As the acrylodan-peptide becomes phosphorylated, it undergoes a time-dependent decrease in its fluorescence at 480 nm. Changes in the rate of this decrease in fluorescence correspond to different rates of PKC-dependent phosphorylation. The figure also shows the time course of the fluorescence of the acrylodan-peptide alone (bullet). The data are expressed as the mean values ± S.E. (n = 6). From 500-1200 s, was significantly different from box or bullet.




DISCUSSION

Our data show that the prodynorphin gene was expressed in adult rat ventricular myocytes and that prodynorphin mRNA was translated into a biologically active end product. The observation that the levels of ir-dyn B were significantly higher in the culture medium than in the myocytes suggests that in the ventricular myocardial cell, which lacks secretory granules(37) , the prodynorphin-derived peptides may be constitutively released shortly after synthesis. PMA elicited a marked increase in the expression of prodynorphin mRNA within the first 4 h of treatment, which was associated to a significant increase in levels of ir-dynB both in the cells and in the culture medium. The finding that these effects of the phorbol ester were inhibited by staurosporine suggests that prodynorphin mRNA induction by PMA was a PKC-mediated event that was associated to an increase in mRNA translation into the biologically active peptide product. A role for PKC in prodynorphin mRNA induction is also supported by the observation that the levels of this mRNA were down-regulated to control values following prolonged exposures to PMA, a treatment that is known to result in depletion or down-regulation of PKC(38, 39) . PKC involvement in the regulation of prodynorphin gene expression is further supported by the finding that the inactive phorbol ester 4alpha-phorbol 12,13-didecanoate did not alter both prodynorphin mRNA and dyn B levels. The nuclear run-off experiments performed in myocardial nuclei isolated from PMA-treated cells indicate that the level of PMA-mediated stimulation of prodynorphin mRNA expression was transcriptional. Specificity and PKC dependence of this stimulatory action of PMA are suggested by the lack of an effect by 4alpha-phorbol 12,13-didecanoate and by the capability of staurosporine to suppress the increase in transcription due to myocyte exposure to the phorbol ester. However, PMA increased nuclear transcription of the prodynorphin gene even when it was directly applied to isolated nuclei. This direct effect of PMA also appears to be specific in nature, because it was not observed following exposure of isolated myocardial nuclei to the inactive phorbol ester 4alpha-phorbol 12,13-didecanoate. The observation that staurosporine was capable of abolishing the effect produced by PMA in nuclei that have been subjected to direct treatment with the phorbol ester suggests that myocyte nucleus may harbor PKC and that activation of this nuclear PKC may be responsible for the stimulation of prodynorphin gene transcription. The presence of PKC at nuclear level was confirmed by the discovery of PKC- and PKC- in the nucleus of untreated myocytes. These results are in agreement with other studies that used immunofluorescent techniques to determine the subcellular localization of different PKC isozymes in cardiac myocytes, showing that PKC- and - immunostaining patterns were mainly detectable in the nucleus of unstimulated cells(40) . Failure to detect PKC-beta and PKC- in our myocyte preparation is consistent with a previous study that utilized a reverse transcriptase-polymerase chain reaction approach and reported the absence of both PKC-beta and PKC- transcripts in both neonatal and adult cardiomyocytes(41) . Further, Kosaka et al.(42) failed to detect any PKC-beta following chromatographic resolution of PKC activity from heart extracts.

The ability of isolated nuclei to phosphorylate a specific PKC fluorescent substrate when a PKC activator was added and the finding that among the different PKC isozymes expressed in the myocardial cell, only PKC- and PKC- were detected in the nucleus suggest that these two PKC isotypes may play an important role in regulating gene expression in the myocardial cell and may be part of the signal transduction pathway involved in the stimulation of prodynorphin gene transcription observed when PMA was directly added to intact isolated nuclei. The phosphorylation of the MARCKS peptide by ``nuclear embedded'' PKC also seems to indicate that the biochemical machinery necessary for activation of PKC is present in the myocardial nucleus. This hypothesis is in agreement with other studies showing that several enzymes and substrates associated with diacylglycerol production are present in the nuclei of rat liver (43) and that inositolphospholipids are synthesized in nuclei of Friend cells(44) . Whether the PKC here described in isolated myocardial nuclei might be activated by endogenous factors remains to be defined. However, receptors for prolactin and various growth hormones have been identified in isolated nuclei from rat liver and splenic mononuclear cells, and these agonists have been shown to increase the activity of a nuclear PKC to a similar extent of that elicited by PMA(45) , suggesting that the phorbol ester effect might be mimicked by endogenous agonists and that PKC activation through specific nuclear receptors might by involved in the regulation of nuclear events, including gene transcription.

The implications of the results of the present report, showing an increase in prodynorphin gene expression following the activation of PKC in myocardial nuclei are still unknown. Nevertheless, the stimulation of nuclear PKC, by eliciting increased prodynorphin gene expression and release of a biologically active opioid peptide, may stimulate myocardial opioid receptors, resulting in the activation of autocrine or paracrine mechanisms that might trigger previously described effects of opioid peptides on myocardial Ca and pH homeostasis as well as on the inotropic state of the cardiac myocyte(4, 5) . Moreover, in several tissues endogenous opioids have been shown to act on plasma membrane opioid receptors in such a way to inhibit cell proliferation and promote cell differentiation(46, 47) . We cannot exclude that myocardial opioid gene expression and its stimulation via nuclear PKC may also play an autocrine role in the regulation of myocardial cell growth and differentiation.

The mechanisms by which PMA-mediated stimulation of nuclear PKC may affect the expression of the prodynorphin gene have not been investigated in the present study. It has been hypothesized that a potential mechanism by which PKC may affect gene transcription is the phosphorylation of RNA polymerase or the phosphorylation of specific nuclear proteins acting as transcription factors(48, 49) . However, nuclear events initiated by PKC activation, including the activation of RNA polymerases, have been reported to be long-lived and to persist after the down-regulation of PKC(50) , indicating that a role for the regulation of transcription by phosphorylation-dephosphorylation remains to be defined. The identification of possible molecular mechanisms involved in PMA effect on gene transcription is further complicated by the finding that different consensus sequences that function as PMA-responsive elements have been identified in phorbol ester-inducible genes and by the fact that DNA-binding proteins that are part of the PMA-activated signal transduction network have been only in part identified(51) . Therefore, the mechanism(s) by which the expression of the prodynorphin gene is regulated by PKC in the myocardial cell remains to be elucidated.


FOOTNOTES

*
This work was supported by Grant 593 from Telethon-Italy. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
To whom correspondence should be addressed. Tel.: 39-79-228278 or 39-79-228279; Fax: 39-79-228120.

(^1)
The abbreviations used are: PKC, protein kinase C; PMA, phorbol 12-myristate 13-acetate; dyn B, dynorphin B; ir-dyn B, immunoreactive dynorphin B; TES, N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid; MARCKS, myristoylated alanine-rich protein kinase C substrate.


ACKNOWLEDGEMENTS

We thank Giuseppe Delogu for excellent technical assistance.


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