Vol. 270, p. 21539
Page 21542, Fig. 4: The reproduction of panels B-D was inadequate. An improved version is shown below with corrected figure legend.
Figure 4:
A, amino acid
sequence comparisons of human EDN, marmoset EDN (mEDN), and human ECP. Numbered boxes enclose the conserved catalytic residues
(His, Lys
, His
); boxes with bold outline enclose the conserved cyste
ines. Open rectangles denote residues shared by EDN and mEDN but not
by ECP and residues shared by ECP and mEDN but not by EDN; open
squares enclose those residues unique to mEDN. The percentage
similarity of mEDN to both EDN and ECP, as well as the calculated
isoelectric point of each sequence are listed in the final columns. B, Western blot containing total cell extracts of IPTG-induced
bacterial transfectants probed with M2 mAb. Lane 1,
recombinant ECP; lane 2, recombinant EDN; lane 3,
recombinant mEDN. C and D are identical blots probed
with polyclonal anti-EDN and anti-ECP antisera, respectively. E, percentage of CFUs of S. aureus surviving after a
4-h incubation at 37 °C with recombinant EDN (open
circles), recombinant mEDN (filled circles), and
recombinant ECP (open squares). Each point represents the
average of triplicate samples; error bars as
indicated.