(Received for publication, July 12, 1995; and in revised form, August 24, 1995)
From the
The fourth conserved domain of the human immunodeficiency virus
type 1 (HIV-1) envelope, the C4 region of glycoprotein 120 (gp120), is
an amphipathic stretch of amino acids that, based on mutational
analyses, constitutes a major component of the CD4 binding region in
gp120. In the absence of crystallographic and NMR data on C4 in intact
gp120, we sought to gain insight into C4's conformation and
accessibility in gp120 by taking an immunochemical approach. In this
study, a peptomer composed of a repeat peptide of C4 amino acids
419-436 from gp120 of HIV-1 was synthesized for use
as a conformationally constrained immunogen. Circular dichroism studies
disclosed that the polymerized peptide, peptomer-(419-436), in
0.01 M Na
HPO
buffer, pH 7.4, at 25
°C contained a dominant
-helical structure (53 ± 1%)
compared with 2 ± 4%
-helical content for the monomeric
peptide-(419-436). The peptomer in Ribi's adjuvant induced
the production of rabbit antibodies that recognized recombinant and
native gp120 but, consistent with the literature, the C4 peptide having
no conformational constraints did not. The experimental results show
that only those antibodies formed against a helical immunogen from C4
will recognize recombinant and native gp120, and, therefore, the
results support the notion that C4 is an
-helix in gp120.
Glycoprotein 120 (gp120) ()of human immunodeficiency
virus type 1 (HIV-1) is the surface glycoprotein that binds to the CD4
receptor on cells in the first step of a cascade of molecular events
leading to HIV infection(1, 2, 3) . The
primary site on gp120 that binds to CD4 resides in the fourth conserved
(C4) domain (4) , and it has been shown that a change in C4 at
a single tryptophan residue could abrogate gp120 binding to
CD4(5) . The virus carrying a mutation at this position in
gp120 is noninfectious(5) . Although amino acid changes outside
C4 also may interfere with the CD4 binding ability of
gp120(6) , Pollard et al.(7) found that 41%
of the gp120 residues were not necessary for CD4 binding, and evidence
was presented that suggested the existence of a minimum CD4 binding
region in gp120.
Despite the volumes of additional published information that describe various aspects of the gp120-CD4 interaction, the exact role of C4 in binding CD4 is not understood, partly because a synthetic C4 peptide has no biological activity. However, answers to key questions about HIV vaccines and therapeutics hinge on understanding the molecular nature of the C4-CD4 interaction.
Because there are no available physical data to describe precisely the conformation of C4 in the intact gp120 we have followed the lead of others (8) and relied on a theoretical treatment of a sequence of amino acids from C4 to assess what the conformation might be. Since most short synthetic peptides have very little, if any, conformation, it was clear that attempts to construct an immunologically active synthetic peptide from C4 having defined conformational constraints was warranted. Antibodies made to defined conformationally constrained immunogens could be used to probe conformationally constrained epitopes that reside in the parent gp120.
It is well known in the field of
polymer physical chemistry that increasing chain lengths of certain
polymers often result in an increase in -helicity(9) . For
the work described here, we investigated the influence that
head-to-tail polymerization would have on the conformation of the
amphipathic peptide from C4. A single method to enhance conformations
of peptides from the C4 region to mimic those conformations found in
the native gp120 could, in principle, be applied to diagnostics,
therapeutics, and vaccine development. We also studied the
immunological activities of the new construct to see if they were
compatible with the intact gp120.
As far as we know, the following is the first description of a construct made of a head-to-tail polymerized peptide, which has a defined conformation and immunogenicity/antigenicity that are considerably different than those of the monomeric peptide. As such, the defined construct may present the peptide in an average conformation that is close to that found for the peptide in the parent protein. This could be an advantage over using monomeric peptides alone or peptide monomers conjugated to carriers since most peptides have very little, if any, conformation. The approach described here can be extended beyond HIV to study many other biological systems that possess important helical conformations within a parent protein's overall conformation.
Because a
single tryptophan in C4 appears to be central to the CD4 binding
ability of gp120(4, 5) , we concentrated our synthetic
efforts on constructing conformationally constrained peptides from the
amphipathic C4 sequence of gp120 that contain Trp-427 at
the central position.
The syntheses of the peptides were performed on an Applied
Biosystems, Inc. (Foster City, CA) model 430A automated peptide
synthesizer. All chemicals used in the peptide synthesis were from
Applied Biosystems, Inc. Recombinant gp120 was a gift
from Genentech, South San Francisco, CA. gp120
from
supernatant media of virus-producing cultures was prepared as
described(10) .
The dimer of the
peptide-(419-436) was synthesized by ligating 20 mg of N-acetyl-peptide-(419-436)-Cys-NH to 20 mg
of ClCH
CO-peptide-(419-436)-NH
. The
ligation reaction was performed in 0.01 M NaHCO
buffer at 25 °C, and the reaction was judged to be complete
by HPLC and by the complete disappearance of the free sulfhydryl groups
as described in (14) . The dimer was purified on a C18 (25
250 mm) HPLC column (Vydac) with a 0.1% aqueous trifluoroacetic
acid stationary phase and a gradient of 0-70% acetonitrile over
30 min at 20 ml/min.
Two rabbits received each immunogen. New Zealand White rabbits (Hazelton, McClean, VA) weighing on average 1.3 kg were immunized by injecting 1 ml of the immunogen subcutaneously in a single site under the left scapula. Three weeks following the initial immunization the rabbits were boosted with an injection under the right scapula. A second boost was administered under the left scapula 6 weeks later. Test bleeds were taken 10 days after the final boost.
Peptomers are polymers of specifically cross-linked synthetic peptides and are formed from cysteine-containing peptides that first are bromoacetylated or chloroacetylated at the N terminus(11, 12, 13, 14, 15) . Fig. 1shows the schematic for the synthesis of peptomer-(419-436) starting with the chloroacetyl-, cysteine-derivatized monomer. Peptomer-(419-436) displays a distribution of molecular weights as seen with SDS-PAGE and staining with Coomassie Blue (Fig. 1, inset).
Figure 1: Schematic of the synthesis of peptomer-(419-436) derived from the C4 of gp120 MN isolate. The syntheses of peptomers beginning with haloacetyl, cysteine-containing peptide starting materials have been described in detail previously (11, 12, 13, 14, 15) . Boxed inset: lane A, sodium dodecyl sulfate-polyacrylamide gel Coomassie Blue-stained peptomer-(419-436); lane B, molecular weight standards.
When the
peptide sequence is placed into the helical wheel described by Schiffer
and Edmondson(16) , it becomes clear that there are two sides
to the resulting conformation, a hydrophobic side and a hydrophilic
side (not shown). This theoretical treatment indicates that under the
appropriate constraints, such as those found in an intact protein, the
peptide could be in an -helical conformation. By equating the Trp
at residue 427 as being n, the center of the hypothetical
-helix, the formula of n, n ± 3, and n ± 4 for placing hydrophobic residues at appropriate
positions for making an
-helix (16) can be demonstrated
here. For the example shown, if Trp-427 is equal to n, then n - 3 is Ile, n - 4 is Ile, n + 3 is Val, and n + 4 is Gly. With the
exception of Gly at the n + 4 position, all amino acids
in the formula n, n ± 3, n ± 4
are hydrophobic, indicating that in the protein residues 419-436
contain sufficient spacing of hydrophobic amino acids to support an
-helix.
A study then was performed to compare the circular
dichroism (CD) spectra of a peptide consisting of amino acids
419-436 from the MN isolate of HIV-1 to the
peptomer-(419-436). Fig. 2demonstrates that, although the
sequences of the constitutive peptides are identical, polymerizing
peptide-(419-436) from the N terminus to the C terminus results
in the formation of a material having a dominant -helical
structure that is considerably greater than that of the free peptide.
From the CD spectra shown, we calculate that peptide-(419-436) in
water contains 2 ± 4%
-helical content, whereas the
peptomer-(419-436)-containing solution contains 53 ± 1%
-helical content. Secondary structure percentages were calculated
as described (17) and are summarized in the inset of Fig. 2. In an effort to gain an understanding of the minimal
requirements for inducing the peptide-(419-436) to take on a
helical conformation, we synthesized elongated fragments of the peptide
starting at the N terminus. It appears that 2 amino acids added to the
N terminus through a thioether linkage result in a peptide that begins
to show helix (6 ± 1%, data not shown), and by dimerizing the
peptide, the percent helix increases to 45 ± 1% (Fig. 2).
Figure 2:
Conformational studies of the C4 peptide
constructs. CD spectra of N-Ac-dimer-(419-436)
(--), peptomer-(419-436) (),
and N-Ac-peptide-(419-436)(- - -). The
percent conformation,
-helix,
-sheet, and unassigned (random)
are listed in the table inset for each
construct.
The effect of polymerization on the conformation of a scrambled
version of peptide-(419-436) and the influence of Trp-427 on the
peptomer conformation were studied. The CD spectrum of the scrambled
peptomer demonstrates an increase in -helical structure compared
with peptide-(419-436), but this increase in conformation was not
as pronounced as it was with peptomer-(419-436) (data not shown).
The percent helix was not calculated for the scrambled peptomer, but it
appeared to be approximately 15% helix when judged in comparison with
the other peptomers' CD spectra.
Tryptophan-containing
synthetic peptides often are synthesized using a formyl group on the
tryptophan residue to protect the tryptophan from oxidation. The
conformation as judged by the CD spectrum of peptomer-(419-436)
containing a formyl-protecting group on the central Trp ((CHO-Trp-427)
in place of Trp-427) resembles the peptide-(419-436) CD spectrum.
These results point to Trp-427 as having an important function in the
maintenance of the -helix of the peptomer in aqueous solutions
that are devoid of molecules known to stabilize helices such as
trifluoroethane or certain detergents.
The influence of Ribi's
adjuvant on the conformation of an immunogen is unknown. However, we
found that when the helical C4 construct is used to immunize rabbits in
Ribi's adjuvant, an antiserum that recognized native (Fig. 3, A1 and A2) and recombinant
gp120 (not shown) was produced. The antisera made by immunizing rabbits
with the C4 peptide-(419-436) did not recognize gp120 (Fig. 3, C1 and C2), and we were unable to
detect any -C4 antibodies in ELISAs with the anti-peptide
antisera.
Figure 3: Reaction of antisera from peptomer-immunized rabbits with recombinant gp120. Rabbit antisera staining strips A1 and A2 were from rabbits immunized with peptomer-(419-436) in Ribi's adjuvant, B1 and B2 were stained with antisera against peptomer-(419-436) using Freund's adjuvant, and C1 and C2 were stained with with peptide-(419-436) antisera in which the rabbits were immunized with the peptide in Ribi's adjuvant. Lane D is the pre-bleed from rabbit A1, and lane E is a blank piece of nitrocellulose. Recombinant gp120 was spotted onto the nitrocellulose in the quantities shown to the left and serially diluted further to the top of the nitrocellulose strip where the final amount of gp120 was approximately 1 ng. Strips were stained with the antisera as described under ``Materials and Methods.''
A single rabbit was immunized with the
CHO-Trp-427-containing peptomer-(419-436) in Ribi's
adjuvant. The antiserum obtained from this rabbit reacted strongly with
the peptide in the ELISA; however, it did not react with native or
recombinant gp120 in the dot blot assay or ELISAs (data not shown).
Because the CHO-Trp-427-containing peptomer-(419-436) contained
no detectable -helix (see above), we interpret this to indicate
that this antiserum was against only a linear portion of the peptomer.
Thus, with Trp at position 427 in the peptomer-(419-436), the conformation of the peptomer is helical, and anti-peptomer antisera binds gp120, recombinant and native. When the Trp at this position is protected with a CHO group, there is no helical conformation in the peptomer, and the anti-peptomer antiserum does not bind recombinant or native gp120. This strongly suggests that gp120 contains a helical epitope comprised of the highly conserved C4 amino acid sequence.
If it is found that the C4 helix is highly conserved among all the HIV-1 strains, this discovery will provide an important common link between conformations shared among all the HIV-1 strains. Because Trp at this position is important for supporting a helical conformation and, as others have shown, the Trp at this position is critical for HIV infection(4, 5) , it may be that a Trp-containing helical conformation in C4 is needed for infection. Aside from amino acid sequence homologies, there have been very few, if any, descriptions of common conformational properties shared by the various strains of HIV-1.
Attempts to perform competition experiments with
the monomeric peptide in ELISA-type formats were uninterpretable for
showing conformational specificity; whereas the peptide in
non-detergent-containing buffers displayed no helical structure as
judged by the CD spectrum, the monomeric peptide did have as much as
20% helical structure in the 0.03% Brij 35-containing buffer that we
use in the ELISA. ()In addition, the peptide itself, when
immobilized to the ELISA plate, strongly reacted with the antisera made
against peptomer-(419-436) in the ELISA, and, because of this, it
was clear that a large amount of the anti-peptomer antisera was against
nonconformationally constrained peptide. An alternative explanation for
the antisera recognizing the peptide would be that the antisera could
be forcing the peptide into a helical conformation upon recognition and
binding to the peptide by the antisera. We presently are not able to
measure the conformation of a peptide or protein immobilized to
polystyrene, so this explanation remains a possibility.
A polymer was synthesized previously using disulfide-linked monomers to form random head-to-tail and tail-to-head polymers(18) . In Freund's adjuvant, this construct was found to produce antibodies in mice that could not recognize the envelope protein gp160(18) . Here, we show that rabbits immunized with peptomer-(419-436) in Freund's also did not produce antibodies that recognized gp120 (Fig. 3, B1 and B2). Freund's adjuvant may be acting to unfold the helix because the paraffin oil and mannide monooleate components of the Freund's may interfere with the contributions of hydrophobic amino acids that are necessary for holding the helix together. Based on these results, we would recommend not using Freund's as an adjuvant for producing antibodies that recognize certain conformationally constrained epitopes.
The dimer of peptide-(419-436) may be a more defined immunogen than the total peptomer mixture because the dimer is purified and is a single molecular entity. We did not include the dimer in the immunization tests because of the possibility, like the monomer, of it being nonimmunogenic due to its low molecular weight. The polystyrene-immobilized dimer did bind the anti-peptomer antisera. However, the antigenic properties of the dimer compared with the peptide or peptomer do not provide any clues about the possible immunogenicity of the dimer.
The literature that addresses the influence of a particular adjuvant on the conformation of an immunogen appears to be very scarce. A paper by Kenney and co-workers (19) evaluated the influence of adjuvants on the quantity, affinity, isotype, and epitope specificity of murine antibodies and, to this end, the immunogens were not characterized from a viewpoint that would address conformation. However, these authors were able to assess several noted differences in the immune responses in mice when various adjuvants were used. These results, showing that adjuvants selectively and independently enhance different qualities of the antibody response, may be based in part on the conformation of the immunogen as presented to the immune system.
With the capabilities that are available today for constructing conformationally constrained peptides as we have demonstrated in this report, the ability to target the immune response to conformationally defined epitopes within a parent protein will become routine as additional examples are reported and the ease with which this is done becomes more obvious. By defining an antigenic material that is used to probe the structure of a parent protein, we should be able to gain useful structural information about complex proteins that could not be obtained through other well established methods such as NMR and crystallography.