©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
Purification and Characterization of the Bifunctional CobU Enzyme of Salmonella typhimurium LT2
EVIDENCE FOR A CobUGMP INTERMEDIATE (*)

(Received for publication, May 24, 1995; and in revised form, August 9, 1995)

George A. O'Toole Jorge C. Escalante-Semerena (§)

From the Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

The CobU protein of Salmonella typhimurium was overexpressed and purified to 94% homogeneity. N-terminal sequencing of purified CobU confirmed the first 22 amino acids. In vitro assays showed that CobU has kinase and guanylyltransferase activities which catalyze the synthesis of adenosyl-cobinamide-GDP from adenosyl-cobinamide, via an adenosyl-cobinamide-phosphate intermediate. We present evidence that the transfer of the guanylyl moiety of GTP to adenosylcobinamide-phosphate proceeds via an phosphoramidate-linked, enzyme-guanylyl intermediate.

In the presence of oxygen, kinase and guanylyltransferase activities of CobU were lost. Treatment of inactive CobU with dithiothreitol restored 20% of the kinase and guanylyltransferase activities, indicating the involvement of sulfhydryl groups in enzyme activity. The sulfhydryl modifying agents 5,5`-dithiobis(2-nitrobenzoic acid) and N-ethylmaleimide abolished both CobU activities.

Native CobU protein was a dimer (40 kDa) that functioned optimally at pH 8.8-9.0 and 37 °C. Substrates and kinetic parameters for both activities were determined. The preferred corrinoid substrate for this enzyme was adenosyl-cobinamide. In vitro experiments are consistent with previous genetic studies which had suggested that adenosyl-cobinamide was the preferred substrate of CobU, and that CobU functioned more efficiently in the absence of oxygen.


INTRODUCTION

Assembly of the nucleotide loop of cobalamin in Salmonella typhimurium is thought to require the involvement of four enzymes, CobU, CobS, CobT, and CobC (Fig. 1). CobT and CobC act sequentially in the generation of dimethylbenzimidazole-alpha-riboside (also known as alpha-ribazole) from nicotinic acid mononucleotide and dimethylbenzimidazole. CobT catalyzes the transfer of the phosphoribosyl moiety of nicotinic acid mononucleotide to dimethylbenzimidazole to yield alpha-ribazole-5`-phosphate(1) , which is then dephosphorylated by CobC to generate alpha-ribazole(2) . Homology to the CobP protein of Pseudomonas denitrificans(3, 4) and nutritional studies performed on S. typhimurium cob mutants (5) suggested that CobU was responsible for the synthesis of Ado^1-CBI-GDP from Ado-CBI, via a Ado-CBI-P intermediate (Fig. 1). The CobS protein is thought to be the cobalamin synthase that catalyzes the synthesis of Ado-cobalamin from Ado-CBI-GDP and alpha-ribazole(2, 4, 5) .


Figure 1: Ado-CBI and the nucleotide loop assembly functions. The top of the figure shows the structure of CBI. X = the 5`-deoxyadenosyl moiety, the structure of which is shown. The pyrrole rings and peripheral ring decorations are labeled. The boxed HO moiety represents the portion of the Ado-CBI modified by CobU-catalyzed reactions. At the bottom of the figure is shown a schematic of the reactions required for assembly of the nucleotide loop of cabalamin. The corrin ring is shown in a simplified form. The asterisks (*) indicates the phosphate donated by [-P]ATP to Ado-CBI. The enzymes catalyzing each reaction are shown.



We report the overexpression and purification of the cobU gene product, document that the CobU protein has both kinase and guanylyltransferase activities required for the synthesis of Ado-CBI-GDP from Ado-CBI, and provide evidence that Ado-CBI-P is an intermediate in the synthesis of Ado-CBI-GDP. We also present evidence that the transfer of the guanylyl moiety of GTP to Ado-CBI-P proceeds via a phosphoramidate-linked, CobUGMP intermediate. The characterization of homogeneous CobU protein includes an analysis of substrate specificity, physical characteristics, and kinetic parameters.

Results from previous genetic studies suggested that oxygen negatively affected CobU activity and that the corrinoid substrate utilized by CobU was also affected by molecular oxygen in the environment(6) . Specifically, it appeared that under aerobic conditions CobU could utilize Ado-CBI only as its corrinoids substrate. These results contrasted with the ability of this enzyme to utilize either Ado-CBI or HO-CBI (i.e. nonadenosylated CBI) under anaerobic conditions (6) .

The in vitro data presented support the hypothesis that the preferred corrinoid substrate for the CobU enzyme is Ado-CBI. HO-CBI can also serve as substrate, albeit poorly. We document oxygen lability of the kinase and guanylyltransferase activities of CobU, both of which can be restored by DTT. Chemical modification of sulfhydryl groups with DTNB or NEM strongly suggested that CobU activities require one or more of the 4 cysteinyl residues present in the protein.


EXPERIMENTAL PROCEDURES

Bacteria, Culture Media, and Growth Conditions

All bacterial strains used were derivatives of S. typhimurium strain LT2, and their genotypes are listed in Table 1. Composition of culture media and growth conditions have been reported(6) . Conditions for the overexpression of cobU were as follows. Strain JE3207 (DEL299 (hisG-cob)/pGP1-2/pJO52) was grown at 30 °C in 1-liter batches of LB medium containing kanamycin (20 µg per ml), and ampicillin (30 µg per ml) in 2-liter Erlenmeyer flasks. Mid-log phase cultures (60 Klett units) were shifted to 42 °C for 40 min, followed by a shift to 37 °C for 12-16 h. Best expression was obtained when incubations (at 37 °C and 42 °C) were performed in water baths maintained in a 37 °C walk-in incubator.



Recombinant DNA Techniques

Unless otherwise noted, all protocols for DNA manipulation were as previously reported(5, 6) .

Overexpression Plasmid

The cobU overexpression plasmid was constructed as shown in Fig. 2. The 700-base pair BamHI-HindIII fragment of pJO7 (cobU) (5) was cloned into pUC118 (7) previously digested with BamHI and HindIII to generate plasmid pJO49. Plasmid pUC118 carries an F(1) origin of replication which can be used to generate single-stranded DNA. Site-directed mutagenesis (8, 9) using the synthetic oligonucleotide 5`-caccagaatCATatgaccggctcc-3` (Eppendorf, Madison, WI) was employed to modify the three bases immediately upstream of the initiating methionine of CobU to generate a NdeI restriction site, yielding plasmid pJO51 (Fig. 2). The NdeI-HindIII fragment of pJO51 was then cloned into pT7-7, previously digested with NdeI and HindIII, yielding plasmid pJO52 (Fig. 2). In this construct, the expression of cobU was under the control of phage T7 promoter and ribosome-binding site.


Figure 2: Site-directed mutagenesis of cobU. At the top of the figure is the wild-type sequence of cobU near its start codon (shown in bold, pJO49) and the stop codon (TGA). The intervening cobU sequence is represented by the four dots. The second line is the mutagenized cobU sequence which results in a NdeI restriction site, shown in capital letters (CATATG, pJO51). The NdeI-to-HindIII (located in the multicloning site, MCS) fragment of pJO51 was cloned into pT7-7 digested with NdeI and HindIII to generate pJO52. In pJO52, cobU is expressed under control of the phage T7 promoter and ribosome binding site.



Synthesis of CobU Corrinoid Substrates

HO-CBI

HO-CBI was synthesized and quantitated as reported without modification(10) .

Ado-CBI

Ado-CBI was synthesized enzymically using a modification of the reported protocol(11, 12) . The reaction mixture contained Tris-HCl, 0.2 M, pH 8.0, at 37 °C; MnCl(2), 200 mM; ATP, 400 mM; CoCl(2), 420 µM; (CN)(2)-CBI, 150 mM. The reaction mixture was made anoxic by bubbling with oxygen-free N(2) gas. A 5-ml sample of the reaction mixture was added to a 25-ml anoxic serum vial containing KBH(4) (0.5 mmol). The vial was kept on ice for approximately 5 min or until the solution turned a black-gray-green color. Crude cell-free extract (approximately 0.2-0.3 mg of protein) made from a strain overexpressing CobA (JE2018) was added, and the vial was transferred to a 37 °C water bath. The reaction mixture was incubated for 2 h, then filtered to stop the reaction and remove particulates. The reaction mixture was then loaded onto a 3.5 times 2.5-cm column of the reverse phase resin LiChroprep RP-18 (EM Separations, Gibbstown, NJ) previously equilibrated with H(2)O. The column was washed with 10 bed volumes of H(2)O, then washed with 2 bed volumes of 100% methanol to elute the Ado-CBI. The sample was concentrated under vacuum (SpeedVac concentrator, Savant Instruments, Farmingdale, NY). All the manipulations were performed in the dark or in dim light to avoid photolysis of the C-Co bond.

The UV-visible spectrum of Ado-CBI showed no significant absorbance at 525 nM, indicating quantitative conversion of (CN)(2)CBI to Ado-CBI (data not shown). The extinction coefficient of Ado-CBI in H(2)O at 457 nm was calculated to be 4,526 M cm. Absorption maxima in H(2)O were observed at 305 and 457 nm.

Synthesis of Radiolabeled Ado-CBI-P

This compound was synthesized in vitro, utilizing the ATP:CBI kinase assay described below. CobU enzyme was added in excess (2.25 µg) to a scaled-up reaction mixture (1 ml) containing [-P]ATP, specific radioactivity = 10 Ci mmol (DuPont NEN), and the reaction was allowed to proceed for 120 min. Radiolabeled Ado-CBI-P was separated from unreacted [-P]ATP by reverse phase chromatography on LiChroprep RP-18 as described above. Specific radioactivity was determined to be 0.0039 Ci mmol. These reaction conditions resulted in quantitative conversion of Ado-CBI to Ado-CBI-P. Unlabeled Ado-CBI-P was generated as described above, except the radiolabeled ATP was replaced with unlabeled ATP.

In Vitro Assays for CobU Activities

ATP:Ado-CBI Kinase Assay

The assay for kinase activity was a modification of a reported protocol(3) . The reaction mixture contained Tris-HCl, 0.1 M, pH 8.8, at 37 °C; MgCl(2), 2.5 mM; ATP, 2 mM; [-P]ATP, 9.96 µM, specific radioactivity = 10 Ci mmol; Ado-CBI, 180 µM; protein, 0.1-0.4 µg; final volume, 20 µl. The reaction mix lacking ATP was prepared on ice and preincubated for 5 min at 37 °C; the reaction was initiated by the addition of ATP and terminated by the addition of 20 µl of 20 mM KCN followed by a 10-min incubation at 80 °C. Samples were centrifuged at 15,850 times g for 10 min in a Marathon 13K/M microcentrifuge (Fisher), and the supernatant was transferred to a fresh tube. One unit of activity was defined as the amount of enzyme required to synthesize 1 nmol of Ado-CBI-P/min. Specific activity was defined as units/mg of protein.

As shown below, ATP did not serve as an efficient substrate for the transferase activity of CobU. Therefore, in the absence of GTP, the conditions for the Ado-CBI kinase assay allowed us to document the synthesis of the intermediate Ado-CBI-P.

GTP:Ado-CBI Kinase, Ado-CBI-P Guanylyltransferase Assay (Protocol A)

This assay was a modification of a reported protocol(3) . The reaction mix contained Tris-HCl, 0.1 M, pH 8.8, at 37 °C; MgCl(2), 2.5 mM; GTP, 2 mM; [-P]GTP, 3.34 µM, specific radioactivity = 30 Ci mmol (DuPont NEN); Ado-CBI, 180 µM; protein, 0.1-0.4 µg; final volume, 20 µl. The reaction mix was handled as described above. One unit of activity was defined as the amount of enzyme required to synthesize 1 nmol of Ado-CBI-GDP/min. GTP served as a substrate for both the kinase and transferase reactions (see below). Therefore, this assay allowed a convenient means to monitor the conversion of Ado-CBI to Ado-CBI-GDP. This assay was used to monitor the activity of fractions during the purification of CobU.

GTP:Ado-CBI-P Guanylyltransferase Assay (Protocol B)

The reaction mix contained Tris-HCl, 0.1 M, pH 8.8, at 37 °C; MgCl(2), 2.5 mM; GTP (or indicated nucleotide), 150 µM; Ado-CBI-P, 300 µM, specific radioactivity = 0.0039 Ci mmol; enzyme, 0.1-0.4 µg in a final volume of 20 µl. The reaction mix was handled as described above. One unit of activity was defined as the amount of enzyme required to synthesize 1 nmol of Ado-CBI-GDP/min.

Quantitation of Ado-CBI-P and Ado-CBI-GDP

In all assays, radiolabeled substrates were diluted with a known amount of unlabeled substrates. To quantitate the amount of product synthesized, the radiolabel incorporated into the product was determined (see below) and adjusted for decay of the radioactivity and counting efficiency of the scintillation counter. The total product synthesized in each assay was calculated using the known ratio of radiolabeled to unlabeled compound, and the specific activity of the radiolabeled compound used.

Protein Concentration Assay

Preliminary protein concentrations were determined by a modification of the turbidimetric method reported by Kunitz(13) . More accurate determinations of protein concentrations were achieved by the BCA method (14) using a commercially available kit from Pierce Chemical Co. (Rockford, IL).

Identification of Products of the CobU-catalyzed Reactions

To identify the products of the CobU-catalyzed kinase and guanylyltransferase reactions, the CobU assay reaction mixtures described above were increased to 100 µl, Ado-CBI was increased to 0.4 mM, in a reaction mixture which contained only unlabeled nucleotide triphosphates. Following a 4-h incubation at 37 °C, products were derivatized to their dicyano forms and purified and analyzed by high pressure liquid chromatography (HPLC). Purified CobU enzyme (94% homogeneous) was used in these reactions.

Chromatographic Methods

HPLC

Products of the large scale CobU-catalyzed reactions were purified by HPLC. The chromatograph used was a computer-controlled Waters model 990 Plus equipped with a photodiode array detector and a model 600 multisolvent delivery system (Waters, Milford, MA).

Purification of (CN)(2)CBI-P

Enzymically synthesized Ado-CBI-P was converted to its dicyano derivative (CN)(2)CBI by addition of an equal volume of 20 mM KCN and heating at 80 °C for 10 min, and purified in two steps. In System 1, the sample was loaded on a BIOSEP-DEAE-P (75 mm times 7.8 mm, Phenomenex, Torrance, CA) column equilibrated with potassium phosphate buffer (0.1 M, pH 6.5) containing 10 mM KCN. The column was washed for 3 min with equilibration buffer, then developed with a 30 min concave gradient (curve 8) of NaCl from 0 to 600 mM in the potassium phosphate buffer (0.1 M, pH 6.5) containing 10 mM KCN. The column was washed with 800 mM NaCl in potassium phosphate buffer (0.1 M, pH 6.5) containing 10 mM KCN for 5 min after the completion of the gradient. Elution of (CN)(2)CBI-P was monitored at 365 nM and the flow rate was 0.3 ml min. In System 2, the column described above was equilibrated with potassium phosphate buffer (0.05 M, pH 6.5) containing 5 mM KCN, and developed with an isocratic system of potassium phosphate buffer (0.05 M, pH 6.5) containing 5 mM KCN at a flow rate of 1 ml min.

Purification of (CN)(2)CBI-GDP

The purification of (CN)(2)CBI-GDP was performed as reported without modifications(5) .

Paper Chromatography

The substrates and products of assays were routinely resolved by ascending paper chromatography on Whatman No. 1 paper (Whatman) in a solvent system of isobutyric acid-water-ammonia (66:33:1)(15) . The solvent front was allowed to migrate between 10 and 15 cm, the paper was dried, and an autoradiograph was prepared. Using the autoradiograph as a guide, each radioactive spot was cut from the chromatograph for quantitation (Packard Tri-Carb 4530, United Technologies, Packard Instruments, Inc., Downers Grove, IL; counting efficiency estimated at 95% for P) in Poly-Fluor (Packard, Meriden, CT) as the scintillation mixture. In some experiments, the relative radioactivity of each radioactive spot was determined by analysis with a PhosphorImager (445I, Molecular Dynamics, Sunnyvale, CA).

Spectroscopic Analysis

UV-visible spectroscopy and fast atom bombardment mass spectrometry were performed as reported elsewhere(1) .

Overexpression and Visualization of CobU

Overexpression of CobU was performed as previously described (16, 17, 18, 19) and visualized by 12% SDS-PAGE(20) , followed by Coomassie Blue staining(19) . The native pI of CobU was determined as reported elsewhere(21) .

Purification of CobU

All purification steps were performed at 4 °C.

Step 1: Preparation of Cell-free Extracts

Cells obtained from 18 liters of the CobU overexpressing strain JE3207 were harvested and resuspended in Tris-HCl, 0.1 M, pH 8.0 at 4 °C containing DTT, 10 mM. Cells were broken by sonication (Branson Ultrasonics Corp., Danbury, CT) at setting 7, 50% duty cycle. Sonication was performed six times at 5-min intervals, and the sample was allowed to cool to less than 15 °C before resuming sonication. Cell debris was cleared from the extracts by centrifugation at 43,140 times g for 1.5 h (Sorvall RC-5B refrigerated centrifuge, DuPont Instruments, Wilmington, DE). Serine protease inhibitor phenylmethylsulfonyl fluoride was then added to a final concentration of 1 mM.

Step 2: Hydrophobic Interaction Chromatography

Finely ground Ultrapure ammonium sulfate (Schwarz/Mann, ICN Biomedicals Inc., Cleveland, OH) was added to crude cell-free extract to 1% saturation. The extract was centrifuged at 10,000 times g for 15 min to remove precipitates. The resulting supernatant was loaded onto a 35-ml bed volume phenyl-Sepharose CL-4B column (Sigma) equilibrated at 4 °C with 1% ammonium sulfate in Tris-HCl, 0.1 M, pH 8.0 at 4 °C, containing 10 mM DTT. The column was washed with 3 bed volumes of the equilibration buffer. CobU was eluted with 50% ethylene glycol in Tris-HCl, 0.1 M, pH 8.0 at 4 °C, containing 10 mM DTT. The flow rate of the column was 30 ml h.

Step 3: Dye Ligand Chromatography

Dye ligand chromatography was used as a negative purification step. Pooled fractions from the phenyl-Sepharose column were dialyzed against Tris-HCl, 0.1 M, pH 8.0 at 4 °C, with 10 mM DTT to remove ethylene glycol. A 15-ml column of Cibacron blue type 3000 (Sigma) was equilibrated with Tris-HCl, 0.1 M, pH 8.0 at 4 °C containing 10 mM DTT plus 5 mM MgCl(2). The dialyzed CobU-containing sample was loaded onto the column at a flow rate of 15 ml h. CobU was recovered from the flow-through and the first bed volume of the wash buffer.

Step 5: Anion Exchange Chromatography

The pooled CobU-containing samples from the previous step was loaded onto a 20-ml column of DEAE Sephadex A-25 equilibrated with Tris-HCl, 0.1 M, pH 8.0, at 4 °C containing 10 mM DTT, at a flow rate of 15 ml h. The column was washed with 2 bed volumes of the equilibration buffer, and eluted with a 5 times bed volume gradient of 0-0.5 M NaCl in the same buffer. CobU activity eluted when the salt concentration was between 300 and 500 mM NaCl and in the 500 mM NaCl wash (data not shown).


RESULTS

Overexpression and Visualization of CobU

Expression of the cobU gene and its gene product was cloned under the control of a strong phage T7 promoter and ribosome binding site. Using the two plasmid phage T7-derived overexpression system(16, 17, 18, 19) , CobU was produced to a level >100-fold higher than chromosomal level produced by the wild-type strain (as judged by densitometry, data not shown). An extract made from a strain carrying the cobU overexpression plasmid (JE3207) showed a prominent band at approximately 22 kDa when analyzed by SDS-PAGE (Fig. 3, Panel A, lane 2). This prominent band was absent from extracts of a control strain (JE3420), which carried only the overexpression vector pT7-7 (Fig. 3, Panel A, lane 1).


Figure 3: Overexpression and visualization of CobU in crude extracts and visualization of purified CobU protein. Panel A, lane 1, crude cell-free extract of a strain carrying only the overexpression vector without any insert (pT7-7, JE3420, negative control). Lane 2, crude cell-free extract of a strain carrying a CobU overexpression plasmid (pJO52, JE3207, CobU overexpressing strain). Panel B, lane 1, size markers in kilodaltons. Lane 2, purified CobU from pooled DEAE fractions (5 µg). CobU is the band indicated by the arrow. Proteins were resolved by SDS-PAGE (12%) and stained with Coomassie Blue.



Activity Assays for the CobU Activities

ATP:Ado-CBI Kinase Activity

Fig. 4, Panel A, shows an autoradiograph prepared from a representative ATP:CBI kinase assay analyzed by ascending paper chromatography. The inclusion of radiolabeled [-P]ATP in the reaction mixture results in the synthesis of radiolabeled Ado-CBI-P (Fig. 4, Panel A, lane 1). In this representative experiment, extracts derived from CobU overexpressing strain (JE3207) had a specific activity of Ado-CBI-P synthesis of 41.5 nmol min mg of protein. This level of activity was approximately 20-fold higher than that measured in extracts made from a strain carrying only the overexpression vector (JE3420, specific activity = 1.9, Fig. 4, Panel A, lane 2).


Figure 4: ATP:Ado-CBI kinase assay and GTP:Ado-CBI kinase, Ado-CBI-P guanylyltransferase assay. Panel A, a representative kinase assay. Panel B, a representative guanylyltransferase assay. In both panels, lane 1 shows the results of assays using an extract generated from the CobU overexpressing strain JE3207. Lane 2 shows the results of assays using an extract generated from strain JE3420, which carries only the overexpression vector pT7-7. The R values for each indicated compound are reported under ``Experimental Procedures.''



The relative mobility of radiolabeled (CN)(2)CBI-P on the chromatograph was 0.77, a value consistent with that reported in the literature(15) . This radioactive product comigrated with authentic (CN)(2)CBI-P; unlabeled (CN)(2)CBI-P was identified on the chromatogram by its characteristic red-purple color (data not shown).

In this paper chromatography system, (CN)(2)CBI migrated with an R(F) = 0.92, ATP migrated with an R(F) = 0.38, and the hydrolysis of ATP resulted in labeled inorganic phosphate which remained near the origin. The synthesis of Ado-CBI-P was dependent on overexpression of CobU and the presence of Ado-CBI and ATP in the reaction mixture. Heat treatment of cell-free extracts resulted in loss of ATP:Ado-CBI kinase activity.

Guanylyltransferase Activity

Fig. 4, Panel B, shows the results of a representative GTP:Ado-CBI kinase, Ado-CBI-P guanylyltransferase assay analyzed by ascending paper chromatography. The inclusion of radiolabeled [-P]GTP in the reaction mixture resulted in the synthesis of radiolabeled Ado-CBI-GDP (Fig. 4, Panel B, lane 1). In this representative experiment, extracts derived from the CobU overexpressing strain (JE3207) had a specific activity of Ado-CBI-GDP synthesis of 218 nmol/min/mg of protein. This level of activity was approximately 400-fold higher than that measured in extracts made from a strain carrying only the overexpression vector (JE3420, specific activity = 0.53, Fig. 4, Panel A, lane 2).

The relative mobility of radiolabeled (CN)(2)CBI-GDP was 0.56, a value consistent with the literature(15) . This radioactive product comigrated with authentic (CN)(2)CBI-GDP and was identified on the paper chromatogram by its characteristic red-purple color (data not shown).

The synthesis of Ado-CBI-GDP was dependent on overexpression of CobU and the presence of Ado-CBI and GTP in the reaction mixture. Heat treatment of cell-free extracts resulted in loss of GTP:Ado-CBI kinase, Ado-CBI-P guanylyltransferase activities.

Ado-CBI-P Is an Intermediate in the Synthesis of Ado-CBI-GDP

As described above, when Ado-CBI was incubated in the presence of CobU and [-P]ATP, radiolabeled Ado-CBI-P was synthesized. The addition of unlabeled GTP to this reaction mixture resulted in the synthesis of radiolabeled Ado-CBI-GDP (data not shown). There were two important implications of these results. First, CobU catalyzed the transfer of the -phosphate of ATP to Ado-CBI to generate Ado-CBI-P. Second, the retention of the label in the product of the reaction (i.e. Ado-CBI-GDP) after addition of unlabeled GTP to the reaction mixture, indicated that the phosphate group donated by ATP was retained in Ado-CBI-GDP. Hence, we concluded that the GMP moiety of GTP was donated to Ado-CBI-P with the release of pyrophosphate. The UV-visible spectrum, and the molecular mass of the cyano derivative of the end product of the guanylyltransferase reaction was consistent with this compound being (CN)(2)CBI-GDP (see below).

Purification and Identification of the Products of the CobU-catalyzed Reactions

Product of the Kinase Reaction

The cyano derivative of the product of the kinase reaction was shown to be (CN)(2)CBI-P. The products of the scaled-up ATP:Ado-CBI kinase reaction using highly purified CobU (94% purity) were analyzed by reverse-phase HPLC in two steps. First, the cyano derivative of the prominent product of the reaction had a (CN)(2)CBI-like spectrum and eluted with a retention time between 7 and 10 min (using System 1, see ``Experimental Procedures''). (CN)(2)CBI-P eluted in a poorly resolved peak adjacent to (CN)(2)CBI. Fractions collected between 7 to 10 min were pooled and analyzed using System 2 (see ``Experimental Procedures''). (CN)(2)CBI-P eluted at 3 min as a single well defined peak. The identity of the prominent peak eluted from System 2 was established as (CN)(2)CBI-P by UV-visible spectroscopy and fast atom bombardment mass spectrometry (data not shown).

Product of the Guanylyltransferase Reaction

The product of the CobU-catalyzed GTP:Ado-CBI kinase, Ado-CBI-P guanylyltransferase activities was shown to be Ado-CBI-GDP. The product of these reactions was analyzed by reverse-phase HPLC after derivatization with KCN; the prominent peak comigrated with authentic (CN)(2)GDP on ascending paper chromatography. Verification of the identity of the product of the guanylyl transfer reaction was established by UV-visible spectroscopy and fast atom bombardment mass spectrometry (data not shown).

Purification of CobU

Fig. 3, Panel B, lane 2, shows a sample of purified CobU as analyzed by SDS-PAGE (12%). Molecular weight size markers are shown in lane 1. The CobU protein was 3-4-fold enriched after these purification steps (Table 2) and >94% pure as judged by densitometry (data not shown).



Some Properties of the CobU Enzyme

Under the conditions described above, both kinase and guanylyltransferase activity assays were shown to be linear with time (for at least 30 min) and with protein (0.1-0.4 µg of protein). The optimal pH for both the kinase and guanylyltransferase activities was between pH 8.8 and 9.0, but over 50% of both CobU activities were retained between pH 8.5 and 9.5 (data not shown). The optimal reaction temperature was 37 °C, but CobU retained at least 50% of its activities when assayed between 25 and 60 °C. Preincubation of CobU at temperatures up to 60 °C (for 10 min), resulted in little effect on either activity (subsequently determined at 37 °C). Preincubation at temperatures above 60 °C resulted in drastic decreases in both CobU activities (data not shown).

The molecular mass of homogeneous CobU, as judged by SDS-PAGE, was estimated at 22 kDa, a value in agreement with a previous report(5) . The predicted mass of CobU, based on the DNA sequence of the cobU gene, is 19.7 kDa(4) . As determined by nondenaturing gel electrophoresis(22) , the native molecular mass was estimated to be 49 kDa. These data were consistent with CobU functioning as a dimer. The experimentally determined isoelectric point of the native CobU enzyme identified two species at pI of 7.2 and 7.5, a sharp departure from the pI predicted for the denatured protein (pI = 5.3). The molar extinction coefficient of CobU at 280 nm in 0.1 M Tris-HCl, pH 8.0 at 4 °C, was 52,205 M cm. The first 22 amino acid residues of the purified CobU protein, as determined by N-terminal sequencing, were identical to the amino acid residues predicted from the DNA sequence of cobU.

Kinetic Parameters

Kinetic parameters for the kinase and guanylyltransferase activities were determined in order to optimize assay conditions (Table 3). The V(max) values for both the kinase and guanylyltransferase reactions were consistently 75% lower when the concentration of the NTP varied than when the concentration of the NTP was fixed. Because most or all this difference in these values could be accounted for by the standard deviation values, it was inferred that the data reflect some variability in the assay. However, additional kinetic studies may be required to fully evaluate these results.



CobU Substrates

Corrinoids

Only Ado-CBI served as an efficient substrate for the synthesis of Ado-CBI-P or Ado-CBI-GDP (Table 4), a result consistent with genetic studies(6) . HO-CBI also served as substrate for CobU, albeit poorly. An approximately 14-fold decrease in the specific activity was measured for the synthesis HO-CBI-P or HO-CBI-GDP from HO-CBI as compared to Ado-CBI. (CN)(2)CBI was also an extremely poor substrate for both CobU activities. These results were consistent with the critical role of the upper ligand for CobU activity.



Triphosphate Nucleotides

Table 4summarizes the results of assays using various nucleotide triphosphates as substrates for the kinase and nucleotidyltransferase activities.

An examination of the CobU amino acid sequence revealed two partial nucleotide triphosphate binding domains(5) . Near the N terminus of CobU there is a portion of the ATP binding domain which specifies phosphate binding, suggesting some flexibility in substrate binding. Consistent with this observation, either ATP or GTP served as phosphate donors for the synthesis of Ado-CBI-P. In fact, the specific activity for the kinase reaction was approximately 10-fold higher when GTP was used as substrate than when ATP was provided at the same concentration. These results did not necessarily imply that GTP was the phosphate donor in vivo, because these increased reaction rates could be the result of an active guanylyltransferase function. For example, the presence of 100 µM unlabeled GTP stimulated the [-P]ATP-dependent kinase activity of CobU approximately 3-fold (data not shown). A similar effect was reported by Blanche et al.(3) for the CobP protein of P. denitrificans. The stimulation of the kinase activity by GTP makes it difficult to determine the preferred substrate for the kinase reaction. Blanche et al.(3) suggested that ATP was the preferred substrate for the CobP protein of P. denitrificans based on kinetic arguments. The amino acid and functional similarity between the CobU and CobP proteins makes it likely that CobU also preferentially utilizes ATP in the kinase reaction.

Near the C terminus of CobU a portion of a GTP binding domain conferring guanine specificity can be identified. This partial GTP binding domain probably represents the portion of CobU required for binding the substrate of the transferase activity. Consistent with this observation, GTP (but not ATP, UTP, or CTP) served as an efficient substrate for the CobU transferase activity (Table 4).

Evidence for the Formation of a CobUGMP Intermediate

The evidence presented above demonstrated that CobU can catalyze the transfer of the GMP moiety of GTP to Ado-CBI-P. The enzyme galactose-1-phosphate uridylyltransferase catalyzes a reaction that is analogous to the reaction catalyzed by CobU, that is, the transfer of the the UMP moiety of UDP-glucose to the phosphorylated substrate galactose-1-P. Frey and co-workers have reported evidence that galactose-1-phosphate uridylyltransferase performs this reaction via an enzyme-bound uridylyl intermediate(23, 24, 25) .

To test whether CobU forms an CobUGMP intermediate, we performed the experiment shown in Fig. 5. As shown in lane 2, Panels A and B, the incubation of CobU with [alpha-P]GTP resulted in substantial radioactivity associated with the CobU protein band. Incubation of CobU with [P]GTP labeled in the -position instead of the alpha-position resulted in no radiolabel associated with CobU (lane 1, Panels A and B). Lanes 3-5, Panels A and B, demonstrated that both CobU and [alpha-P]GTP were required for the formation of the radiolabeled protein band.


Figure 5: Evidence for the formation of a CobUGMP intermediate. Panel A, the PhosphorImager analysis of the polyacrylamide gel shown in Panel B. The experiment was performed as follows. The indicated additions were incubated in Tris-HCl (0.1 M, pH 8.8, at 37 °C) plus 2.5 mM MgCl(2) in a final volume of 10 µl. After a 5-min incubation at 37 °C, 2 µl of 6 times SDS-PAGE loading buffer were added, and the sample was heated to 80 °C for 10 min and immediately analyzed by SDS-PAGE (12%). The amount of each addition was: CobU, 2.5 nmol; [alpha-P]GTP, 25 pmol (specific radioactivity = 800 Ci mmol); and [-P]GTP, 25 pmol (specific radioactivity = 30 Ci mmol). Panel B, the reaction mixtures analyzed by SDS-PAGE (12%) and stained with Coomassie Blue. Size markers are shown at the left and labeled with their molecular mass (kDa).



Inclusion of a 100-fold molar excess of unlabeled GTP (Fig. 5, Panel A, lane 6) resulted in a 30-fold decrease in label incorporated into the CobU protein when compared to lane 2. The inclusion of a 100-fold excess of unlabeled ATP resulted in only a 0.4-fold decrease in label incorporated into the CobU protein. As described above, both ATP and GTP can serve as substrates for the kinase activity, but only GTP is an efficient substrate for the guanylyltransferase activity. The ability of GTP (but not ATP) to compete with the radiolabeled GTP suggested that the association of radiolabel with CobU occurred at a site required specifically for GTP binding and presumably for the guanylyl transfer reaction.

Based on the data presented in this section, it is formally possible that CobU forms an enzyme-GDP intermediate. Two lines of evidence argue against such a conclusion. The cyano-derivative of product of the CobU-catalyzed reaction has a molecular mass identical to (CN)(2)CBI-GDP. Further, the phosphate moiety of Ado-CBI-P was retained in Ado-CBI-GDP, suggesting that a GMP moiety (not a GDP moiety) was transferred to Ado-CBI-P to generate Ado-CBI-GDP. Taken together, these data indicated that the GMP moiety of GTP, not the GDP moiety, was transferred to the CobU enzyme.

The data presented above indicated that GMP was associated with the CobU protein. Further, the label associated with CobU was retained after treatment of the protein with SDS, heating to 80 °C, and analysis by SDS-PAGE. These observations indicated that GMP had formed a stable covalent bond with the CobU protein. This point is addressed in more detail in the next section.

The Sensitivity of the CobUGMP Intermediate to Acid and Stability to Base Is Consistent with a Phosphoramidate Linkage

We suspected that GMP had formed a covalent bond to the CobU enzyme. This linkage could be to the carboxyl group of Glu or Asp, the -amino group of Lys, or the imidazole side chain of His. To distinguish among these possibilities we assessed the stability of the CobUGMP to acid and alkali conditions, using reaction conditions similar to those reported(26) .

Fig. 6, lanes 1 and 2, shows that a 10-min incubation at 55 °C (with no addition of acid or base) resulted in CobU protein which retained the radiolabeled GMP. Treatment with acid (pH of 1.5) at 55 °C for 10 min resulted in a 38-fold decrease in radiolabel associated with the CobU protein (lanes 3 and 4). The CobU protein, however, was still visible on Coomassie Blue-stained polyacrylamide gels at levels comparable to the control lanes (data not shown). Treatment with base (pH of 13.0) resulted in no loss of radiolabel (lanes 5 and 6).


Figure 6: Stability of the CobUGMP intermediate to acid and alkali treatment. Shown is the PhosphorImager analysis of samples analyzed by SDS-PAGE (12%). Lanes 1 and 2 contain the H(2)O-treated control reaction mixture. Lanes 3 and 4 contain the acid-treated samples. Lanes 5 and 6 contain the alkali-treated samples. The position of CobU is identified by the arrow. The reactions were performed as follows. Radiolabeled CobUGMP intermediate was generated as described in Fig. 5, except after 5 min at 37 °C, 2.5 µL of H(2)O, 4 M NaOH, or 4 M citric acid were added to the indicated reactions. After incubation for 10 min at 55 °C, 2.5 µl of H(2)O, 4 M HCl, or 4 M NaOH, respectively, were added to neutralize the reactions. The samples were analyzed by SDS-PAGE (12%), followed by analysis with the PhosphorImager.



These data showed that the CobUGMP intermediate was sensitive to treatment with acid, but not to treatment with base. These data argue against a phosphoester bond, which should be sensitive to both acidic and alkali conditions. This behavior was consistent with a phosphoramidate linkage.

The CobUGMP Intermediate Drives the Synthesis of Ado-CBI-GDP

The data above indicated that incubation of CobU with GTP resulted in the formation of an enzyme-bound guanylyl intermediate. However, we had not yet established whether this intermediate could drive the synthesis of Ado-CBI-GDP if provided with its other substrate, Ado-CBI-P.

To address this point, we performed the experiment described in Table 5. Briefly, the CobUGMP intermediate was generated and dialyzed against buffer to remove unreacted [alpha-P]GTP. As shown in Table 5, the purified CobUGMP intermediate was capable of driving Ado-CBI-GDP synthesis only if provided with Ado-CBI-P, or Ado-CBI and ATP. These results allowed two conclusions. First, the CobUGMP intermediate was functional for the synthesis of Ado-CBI-GDP. Second, the CobUGMP intermediate was competent for the synthesis of Ado-CBI-P from Ado-CBI and ATP. From this second observation we inferred that the two activities of the protein function independently, a result consistent with genetic experiments. (^2)



Each CobU Monomer Binds a GMP Moiety

As described above, CobU probably functions as a dimer. We were were interested in determining whether both monomers of the CobU dimer were capable of catalyzing a guanylyl transfer. To test this idea, 1.25 pmol of CobU dimer were incubated in the presence of a 10-fold molar excess of [alpha-P]GTP (specific radioactivity = 800 Ci mmol) in a 10-µl reaction mixture containing 2.5 mM MgCl(2) in Tris-HCl, 0.1 M, pH 8.8, at 37 °C. The reaction mixture was incubated at 37 °C for 10 min, then 2 µl of 6 times SDS loading buffer were added, and the samples were incubated at 80 °C for 10 min. Each sample was analyzed by SDS-PAGE (12%), the radiolabeled CobU band was excised, and the radioactivity of the band was determined by scintillation counting. Using the known specific activity of the [alpha-P]GTP, the moles of GMP in each sample were determined.

A ratio of 1.56 ± 0.27 mol of GMP per mol of CobU dimer was calculated based on results from four trials. These data suggested that each CobU monomer bound a GMP moiety and, based on the results in the preceding section, can subsequently catalyze a guanylyl transfer. Based on this result, we propose that both monomers of the CobU dimer are functionally equivalent and can likewise catalyze the kinase reaction.

Reducing Agents Are Required to Maintain CobU Activities

Crude cell-free extracts of strains overexpressing CobU did not require the addition of reducing agents in order to detect either the kinase or guanylyltransferase activities. However, during the initial stages of purification, CobU rapidly lost both of its activities. The addition of 10 mM DTT to the reaction mixture stimulated both kinase and guanylyltransferase activities (data not shown). Therefore, DTT was included in all steps of the CobU purification, and the enzyme was routinely stored under oxygen-free N(2).

Thiol-reducing agents have been reported to react and form complexes with corrinoids(27) . Therefore, it was not clear whether DTT affected the protein, the corrinoid substrate, or both. To address this concern we performed the following experiment. A sample of purified CobU was stored at 4 °C in air for approximately 2 weeks. The kinase and guanylyltransferase activities of this sample decreased to <1% of their original activity (the specific activity for the kinase reaction decreased from 83 to 0.7, and for the guanylyltransferase reaction the specific activity decreased from 76 to 0.4). This form of CobU enzyme was referred to as ``oxidized CobU.'' Enzyme stored at 4 °C under a N(2) headspace retained >98% of its activity for at least 3 weeks (data not shown).

DTT (10 mM) was added to one half of the oxidized CobU sample and incubated on ice for 2 h; an equal volume of H(2)O was added to the other half of the sample. The DTT-treated sample (0.5 ml) was dialyzed against 2 times 1 liter of anoxic Tris-HCl, 0.1 M, pH 8.0 at 4 °C, to remove the DTT (this represents a 4 times 10^6-fold dilution). The untreated sample was dialyzed against the same type and volume of oxic buffer. DTT treatment of oxidized CobU resulted in an 18-fold increase in kinase activity (specific activity increased from 0.7 to 12.6), and a 42-fold increase in guanylyltransferase activity (specific activity increased from 0.4 to 17.0). The activities of the DTT-treated CobU represent 15-20% of the original specific activities. Untreated CobU showed no change in either activity. Addition of DTT to a reaction mixture containing reduced CobU enzyme did not further stimulate activity, suggesting that the effect of DTT was due to the reduction of disulfide bonds in the enzyme (data not shown).

We quantitated the total moles of sulfhydryl groups in reduced and oxidized CobU by the method of Habeeb(28) . DTT-treated CobU had 4 mol of reduced sulfhydryl groups per monomer of CobU, a result consistent with the 4 cysteinyl residues per CobU polypeptide. Untreated CobU showed <1 mol of reduced sulfhydryl group per monomer of CobU. Therefore, the presence of reduced sulfhydryl groups correlated with active kinase and guanylyltransferase functions of CobU.

Evidence for the Involvement of Sulfhydryl Groups in the Kinase and Guanylyltransferase Activities

To investigate the involvement of sulfhydryl groups in CobU activities we tested the effect of sulfhydryl-reactive compounds on CobU kinase and guanylyltransferase activities.

An analysis of available sulfhydryl groups in the native CobU protein with DTNB was performed as reported elsewhere(28) . We observed a single class of sulfhydryl residues, which were fully modified with DTNB (at 0.33 and 1 mM) in less than 2 min (assays were monitored for a total of 30 min). We determined a value of 4 available sulfhydryl groups per dimer of CobU; each CobU dimer has 8 cysteinyl residues. The rapid modification of DTNB by CobU suggests that the sulfhydryl residues are readily accessible to DTNB. In fact, the rate of modification of CobU by DTNB is similar to the rate reported for small molecules such as cysteine(28) .

We tested whether the sulfhydryl modifying agents DTNB and NEM inhibited CobU kinase and guanylyltransferase activities. CobU was incubated at 37 °C in the presence of 1 mM DTNB and NEM for 10 min, then assayed immediately for its kinase and guanylyltransferase activities. The kinase activity of CobU (original specific activity = 231) was inhibited 95% by treatment with 1 mM DTNB (specific activity = 11) and inhibited 94% by treatment with 1 mM NEM (specific activity = 14). The guanylyltransferase activity (original specific activity = 93) was inhibited 92% (specific activity = 8) and 87% (specific activity = 13) by treatment with DTNB and NEM, respectively. The buffer used to prepare the DTNB and NEM solutions (0.1 M NaP(i), pH 8.0) had no effect on either CobU activity (data not shown). Taken together with the study above, these data suggested that one or more of the rapidly modified sulfhydryl groups is required for CobU kinase and guanylyltransferase activities.

The Effect of Substrates on Inhibition by DTNB

In general, substrates provided little protection from inhibition by DTNB. CobU enzyme was preincubated with individual substrates under typical assay conditions (see ``Experimental Procedures'') at 37 °C for 10 min, then DTNB was added to 1 mM, and the reaction mixes were incubated for an additional 10 min at 37 °C.

Ado-CBI and ATP (for the kinase activity) and Ado-CBI-P (for the guanylyltransferase activity) afforded no protection from inhibition by DTNB. After incubation with substrate and inhibitor, unreacted compounds were separated from CobU on a desalting column of Sephadex G-25. CobU was assayed immediately for the appropriate activity. In all cases, the activity of CobU treated with DTNB in the presence of substrate was no higher than CobU treated with DTNB in the absence of substrate (data not shown). Untreated CobU served as a positive control and retained both activities after the 20 min of incubation at 37 °C and passage over the sizing column (data not shown).

GTP provided slight protection from inhibition of the guanylyltransferase activity. CobU was incubated with 150 µM GTP for 10 min to allow formation of the CobUGMP intermediate, then treated with DTNB as above. Dialysis against 2 times 1 ml of 0.1 M Tris-HCl, pH 8.0 at 4 °C, in a Centricon-10 concentrator removed the unreacted GTP and DTNB, but the CobUGMP intermediate is stable under these conditions (see section on CobUGMP intermediate formation above). GTP-treated CobU (specific activity = 1.25 nmol of Ado-CBI-GDP/min/mg) had 3-fold more activity than the enzyme treated with DTNB in the absence of GTP (0.34 nmol/min/mg). However, control enzyme not treated with DTNB had a specific activity of 50.4 nmol/min/mg. Therefore, even in the presence of GTP, DTNB inhibited the guanylyltransferase activity >95%. This GTP-protected CobU had no detectable kinase activity (data not shown).

The activity of DTNB-modified CobU could be partially restored by treatment with DTT. CobU was treated with DTNB and dialyzed as described above. The enzyme was subsequently incubated with 10 mM DTT at 37 °C for 15 min, then immediately assayed for both kinase and guanylyltransferase activity. The kinase activity was stimulated 4-fold by treatment with DTT (specific activity increased from 1.0 to 3.8 nmol of Ado-CBI-P/min/mg). The guanylyltransferase activity was stimulated 6-fold by treatment with DTT (specific activity increased from 1.1 to 6.4 nmol of Ado-CBI-GDP/min/mg). However, this DTT-mediated increase results in a specific activity 10-fold below that of untreated control enzyme (specific activity = 50.4). There was no increase in either activity when the time of DTT treatment was increased to 60 min (data not shown).

At present, it is not clear whether cysteinyl residues are required for one or both CobU activities or, if so, how many cysteinyl residues are involved. It is possible that a modified cysteinyl residue is required for only one activity, and the loss of the second activity is simply the consequence of an indirect effect(s) of the modifying agents. Site-directed mutagenesis of each cysteinyl residue is in progress to evaluate the involvement of each of these residues in CobU activities.

CobU Does Not Have Detectable ATP:Adenosyltransferase Activity

We had previously shown that CobU plays a key role in the assimilation of nonadenosylated CBI into cobalamin under anaerobic conditions in cobA mutants. cobA mutants lack ATP:corrinoid adenosyltransferase activity(6, 29) . One interpretation of these genetic studies was that CobU had an ATP:CBI adenosyltransferase activity which catalyzed the synthesis of Ado-CBI, the preferred substrate for the CobU enzyme(6) . To test this hypothesis we used homogeneous CobU in ATP:corrinoid adenosyltransferase assays, performed as reported(12) .

Control assays with the ATP:corrinoid adenosyltransferase enzyme (CobA), resulted in the synthesis of Ado-CBI from co(I)binamide and ATP (12) . The synthesis of Ado-CBI was monitored by two methods. In a spectrophotometric assay, the synthesis of Ado-CBI was monitored at 457 nm(12) . In a bioassay, Ado-CBI (but not nonadenosylated CBI) restored growth of a cobA mutant on minimal medium(29) . This bioassay is capable of detecting picomolar quantities of corrinoids. (^3)

When homogeneous CobU (20 µg per assay) was substituted for CobA in the assay, we were unable to detect Ado-CBI synthesis. We concluded that CobU did not have ATP:CBI adenosyltransferase activity in vitro. Alternative models to explain the role of CobU in the assimilation of nonadenosylated corrinoids under anaerobic conditions should focus on the specificity of the enzyme for its corrinoid substrate as a function of oxygen in the environment.


DISCUSSION

CobU Is a Bifunctional Enzyme That Catalyzes the Synthesis of Ado-CBI-GDP from Ado-CBI, via an Ado-CBI-P Intermediate

The CobU protein of S. typhimurium was purified to >94% purity. The experimentally determined N-terminal sequence of the purified protein showed a 22 of 22 match with the amino acid sequence predicted from the DNA sequence of cobU(4, 6) . These data confirmed the purification of CobU and suggested that the N terminus of CobU is not modified.

The kinase activity of CobU catalyzes the phosphorylation of Ado-CBI at the expense of ATP to yield Ado-CBI-P. The -phosphate of ATP is donated to Ado-CBI in this reaction, and remains a part of the product of the CobU-catalyzed reaction, Ado-CBI-GDP. In S. typhimurium, the -phosphate of ATP probably remains as part of the end product of the pathway, Ado-cobalamin(1, 2) .

The second CobU activity catalyzes the transfer of the guanylyl moiety of GTP to Ado-CBI-P. We presented evidence that the transfer of the guanylyl moiety occurs via an enzyme-guanylyl intermediate (Fig. 5). This intermediate is acid-labile and alkali-resistant (Fig. 6). The properties of the enzyme-guanylyl intermediate are reminiscent of the phosphoramidate linkages observed in galactose-1-phosphate uridylyltransferase (23, 24, 25) and histidine kinases such as FixL(26) .

Release of the GMP moiety from the CobUGMP intermediate is driven by the presence of Ado-CBI-P (provided exogenously or generated in situ), but not by Ado-CBI. This finding is consistent with the identification of Ado-CBI-P as an intermediate in the synthesis of Ado-CBI-GDP and supports the sequence of reactions as phosphorylation followed by guanylyl transfer.

Two additional lines of evidence support the bifunctionality of CobU. Overexpression of the CobU polypeptide (Fig. 3, Panel A) results in the overexpression of both kinase and guanylyltransferase activities (Fig. 4) and both enzymatic activities copurified through three chromatography steps to >94% homogeneity (Table 2).

What Residues Are Required for the Formation of the CobUGMP Intermediate?

We have identified an allele of cobU which results in an amino acid substitution at His-46. The H46Y amino acid change results in a CobU protein unable to support cobalamin synthesis in vivo.^2 His-46 is adjacent to a second histidyl residue at position 45. Both residues are conserved between the CobU protein and the CobP protein of P. denitrificans (data not shown). Interestingly, Frey and co-workers identified two nearby histidyl residues in galactose-1-phosphate uridylyltransferase (His-164 and His-166) which were required for the formation of the uridylyl-enzyme intermediate(25) . We are currently using site-directed mutagenesis to determine if His-45 and/or His-46 are required for the formation of the CobUGMP intermediate.

Oxygen Lability of CobU Enzymatic Activities and a Role for Cysteinyl Residues

The loss of CobU activity in vitro in the absence of a reducing agent is consistent with findings from genetic experiments which suggested that CobU functioned more efficiently when cells were grown anaerobically than aerobically(6) . The requirement for DTT to maintain both kinase and guanylyltransferase activities, and the results of DTNB and NEM inhibition studies, is diagnostic of a role for one or more of the 4 cysteinyl residues present in the CobU enzyme. However, it is still not clear if a cysteinyl residue participates at the active site(s) of this enzyme.

Comparison of the CobU amino acid sequence to its homolog CobP in P. denitrificans showed that cysteine at position 81 in CobU is conserved between these two proteins, suggesting an important role of this residue in enzyme activity(ies). This idea has been verified by genetic means. Site-directed mutagenesis of CobU at this position was performed to change the cysteine to alanine (C81A). The C81A allele of cobU resulted in loss of CobU function in vivo and in vitro (data to be presented elsewhere).

Although CobU has 4 cysteinyl residues, CobP has only the single conserved cysteinyl residue and the CobP enzyme does not appear to require reducing agents to maintain its activities(3) . Interestingly, Wong et al.(24) reported that galactose-1-phosphate uridylyltransferase also had an essential sulfhydryl group, probably located outside of the active site of the enzyme. Studies on the involvement of the remaining cysteinyl residues in CobU enzyme activity(ies) is in progress.

Specificity of CobU for Its Corrinoid Substrate

Previously reported genetic studies suggested that CobU could assimilate non-adenosylated corrinoids into cobalamin anaerobically, but not aerobically. We proposed two models to address these findings(6) . One model proposed that CobU was capable of catalyzing the synthesis of Ado-CBI, then utilizing this substrate for the synthesis of Ado-CBI-GDP. The data presented here (i.e. the inability of CobU to catalyze the synthesis of detectable quantities of Ado-CBI in vitro) and the lack of amino acid similarity between CobU and adenosyltransferase enzymes (data not shown) argue against such a model.

An alternative model proposed that CobU could utilize nonadenosylated corrinoids as substrates in the absence of oxygen. Our in vitro data show that CobU can utilize HO-CBI, although Ado-CBI is clearly the preferred substrate. Therefore, it appears that CobU can catalyze the synthesis of CBI-GDP from non-adenosylated CBI, albeit inefficiently. This low level of CBI-GDP synthesis, however, appears to be sufficient to satisfy the cell's requirement for cobalamin under anaerobic growth conditions(6) . Structural work on CobU (currently in progress) will prove valuable in understanding how corrinoid substrate specificity is established by the primary structure of this protein.


FOOTNOTES

*
This work was supported in part by United States Public Health Service, National Institute of General Medical Sciences Grant GM40313 (to J. C. E.-S.) and by the College of Agricultural and Life Sciences of the University of Wisconsin. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
To whom correspondence should be addressed: Dept. of Bacteriology, University of Wisconsin, 1550 Linden Dr., Madison, WI 53706. Tel.: 608-262-7379; Fax: 608-262-9865; jcescala{at}facstaff.wisc.edu.

(^1)
The abbreviations used are: Ado, 5`-deoxyadenosyl; CBI, cobinamide; Ado-CBI-P, Ado-CBI-phosphate; HO, hydroxo; (CN)(2)CBI, dicyano-CBI; (CN)(2)CBI-P, dicyano-CBI-phosphate; alpha-ribazole-5`-P, N^1-(5-phospho-alpha-D-ribosyl)-5,6-dimethylbenzimidazole; alpha-ribazole, alpha-D-ribosyl-5,6-dimethylbenzimidazole; DTT, dithiothreitol; CobUGMP, enzyme-guanylyl intermediate; DTNB, 5,5`-dithiobis(2-nitrobenzoic acid) NEM, N-ethylmaleimide; HPLC, high performance liquid chromatography; PAGE, polyacrylamide gel electrophoresis.

(^2)
G. A. O'Toole and J. C. Escalante-Semerena, unpublished results.

(^3)
J. C. Escalante-Semerena, unpublished results.


ACKNOWLEDGEMENTS

We thank P. A. Frey for his helpful advice in the determination of the kinetic parameters. We thank R. Randall from the Mass Spectrometry Laboratory of the Biochemistry Department at the University of Wisconsin-Madison for obtaining the mass spectra and J. Wallent from the Protein Sequence and Characterization Service of Nucleic Acid and Protein Facility at the University of Wisconsin-Madison Biotechnology Center for protein sequencing. We also thank S. Tabor for plasmids.


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