©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
Rapamycin, Wortmannin, and the Methylxanthine SQ20006 Inactivate p70 by Inducing Dephosphorylation of the Same Subset of Sites (*)

(Received for publication, May 9, 1995; and in revised form, July 10, 1995)

Jeung-Whan Han Richard B. Pearson (§) Patrick B. Dennis (¶) George Thomas (**)

From the Friedrich Miescher Institut, PO 2543, CH4002 Basel, Switzerland

ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

Activation of p70 in cells stimulated with serum correlates with the phosphorylation of seven sites. Pretreatment of Swiss 3T3 cells with the immunosuppressant rapamycin blocks phosphorylation of four of these sites (Thr, Thr, Ser, and Ser), whereas phosphorylation proceeds in the remaining three sites (Ser, Thr, and Ser). If rapamycin is added post-serum stimulation, the pattern of phosphorylation is qualitatively similar except that Ser is still highly phosphorylated. The inhibitory effect of rapamycin on serum-induced p70 activation and the phosphorylation of Thr, Thr, Ser, and Ser is rescued by FK506, providing further evidence that the inhibitory effect is exerted through a complex of rapamycin-FKBP12. Wortmannin treatment pre- or post-serum stimulation inhibits phosphorylation of the same set of sites as rapamycin, supporting the argument that both agents act on the same pathway. Likewise, methylxanthine phosphodiesterase inhibitors block p70 activation and phosphorylation of the same set of sites as wortmannin and rapamycin. However, other agents that raise intracellular cAMP levels have no inhibitory effect, leading to the hypothesis that the inhibitory actions of methylxanthines on p70 activity are not through activating protein kinase A but through inhibition of an upstream kinase. Together the results indicate that there are two kinase signaling pathways that must converge to activate p70 and that only one of these pathways is sensitive to rapamycin, wortmannin, and methylxanthine inhibition.


INTRODUCTION

p70 and p85 represent two isoforms of the same kinase that are encoded by a common gene and are identical except for a 23-amino acid extension at the amino terminus of p85 (see Refs 1 and 2). Furthermore, both isoforms lie on a p21-p42/p44 independent signal transduction pathway(3, 4) , which bifurcates at the level of the receptor(4) . Whereas p70 seems to be restricted to the cytoplasm(5) , the amino-terminal extension of p85 harbors a nuclear localization signal that constitutively targets it to the nucleus(5) . The major substrate of the kinase in both compartments of the cell appears to be the 40 S ribosomal protein S6 (see (6) ), whose multiple phosphorylation in the cytoplasm has been implicated in the selective translational up-regulation of a family of essential gene products(7, 8) . Consistent with this finding, either microinjection of neutralizing antibodies into cells (9) or treatment of cells with the immunosuppressant rapamycin, which selectively blocks p70/p85 phosphorylation and activation (10, 11, 12) , severely impedes cell cycle progression.

Activation of p70/p85 is associated with multiple phosphorylation of the enzyme, which can be monitored as the slower migration of a family of bands on SDS-PAGE, (^1)which collapse into a single band following treatment with phosphatase or rapamycin(10, 13) . Initially, phosphorylation at four major sites, Ser, Ser, Thr and Ser, which are clustered in a putative autoinhibitory domain, was found to parallel p70 activation(14) . Substitution of these four residues with acidic amino acids mimicked phosphorylation at these sites(15) . Unexpectedly, the acidic form of the kinase was still inactivated by rapamycin, and more surprisingly, inactivation was shown to be associated with the dephosphorylation of a distinct set of sites, with no effect observed on the phosphorylation of the four identified sites(15) . Recently, these novel rapamycin-sensitive sites have been identified as Thr in the catalytic domain and Thr and Ser located in a linker domain that couples the catalytic and autoinhibitory domains. (^2)Each of the sites was found to reside in an atypical trypsin cleavage product, which largely explained the difficulty in their identification. Earlier rapamycin studies (15) led to the hypothesis that at least two sets of phosphorylation events regulate kinase activity, one of which was controlled by mitogens and a second that appeared to be constitutively activated. However, in re-examining this data, it was noted that in the presence of serum, the parent and the acidic mutant exhibited a similar mobility shift when analyzed by Western blots of SDS-PAGE(15) . Furthermore, in both cases, rapamycin treatment caused a similar increase in electrophoretic mobility of the protein. These data suggested that one or more of the rapamycin-sensitive phosphorylation sites was involved in the p70 mobility shift(15) . Since a similar mobility shift is observed during the rapid activation of p70 by mitogens(4, 10) , which is blocked by rapamycin (10) , this finding raises the question of whether the rapamycin-sensitive phosphorylation sites are also regulated by mitogens.

Since p70 activation has not yielded to in vitro reconstitution(3, 16) , a number of indirect approaches have been exploited in an attempt to identify key regulatory points in this signaling pathway(4, 17, 18) . The establishment of regulatory points has been extremely valuable in elucidating other signal transduction pathways(19) . In the case of p70, this line of study has led to the finding that the antibiotic wortmannin, which blocks phosphatidylinositol 3-OH kinase activation, also blocks p70 activation(17, 18) . Though the role of phosphatidylinositol 3-OH kinase in regulating this pathway has been questioned(4, 20) , wortmannin clearly inhibits a key step in the p70 pathway. In contrast to rapamycin, wortmannin does not inhibit TPA activation of p70, leading to a model in which the wortmannin block has been placed upstream of the site of rapamycin action(18) . Exploiting a similar line, Monfar et al.(21) have recently demonstrated that raising intracellular levels of cAMP in T cells either blocks interleukin 2-induced p70 activation or causes the immediate inactivation of the kinase in post-interleukin 2-stimulated cells. These findings have led to an expansion of the model above in which activation of protein kinase A acts as a key negative regulator of p70 as well as phosphatidylinositol 3-OH kinase, whose activation is also blocked by raising intracellular cAMP levels(21) . To raise cAMP levels, Monfar et al.(21) co-treated cells with forskolin, an activator of adenylate cyclase, and IBMX, a phosphodiesterase inhibitor. However, methylxanthines, such as IBMX, have been shown to also act as protein kinase inhibitors(22) . Indeed, previous studies have demonstrated that treatment of cells with methylxanthines alone is sufficient to block S6 phosphorylation, whereas raising intracellular cAMP with prostglandin E(1), a potent adenylate cyclase agonist, had no inhibitory effect on this response(23) . These latter findings raise the possibility that methylxanthines themselves may block p70 activation and, if selective, could be potential tools for identifying upstream kinases in this pathway.

The mapping of individual phosphorylation sites involved in regulating p70 enables the assessment of these models and allows for the determination of whether the newly identified sites of phosphorylation are also implicated in p70 activation. Here we have employed p70 mutants as well as two-dimensional phosphopeptide analysis of endogenous p70 to establish whether any of the newly identified rapamycin-sensitive sites of phosphorylation are also involved in mitogenic activation of p70. Next we have assessed whether rapamycin and wortmannin inhibition of p70 activation is paralleled by the dephosphorylation of the same or a distinct set of sites. Finally, we have examined whether methylxanthines are capable of blocking p70 activation, as opposed to other agents that raise intracellular cAMP levels, and have examined the sites of p70 phosphorylation affected by such treatment.


EXPERIMENTAL PROCEDURES

Plasmid Constructs and Mutagenesis

Site-directed mutagenesis resulting in the mutations of Ser Asp or Ala (residues 411, 418, and 424) and Thr Glu or Ala (residue 421) was performed as described previously(15) . To epitope-tag the p70 constructs, we introduced by polymerase chain reaction the sequence GAG CAG AAG CTT ATC TCC GAG GAG GAC CTG, corresponding to the Myc 9E10 epitope (24) after the initiation codon of the wild type p70 (p2B4)(25) . Exchanging Xba-BglII fragments (nucleotides 1-911) between wild type p70 and p70D(3)E or p70A(4) allowed the introduction of the Myc tag in the mutant constructs.

Cell Culture, DNA Transfection, and Radioactive Labeling

Swiss mouse 3T3 cells were grown, maintained, and arrested in G(O) as described previously(26) . For in vivoP(i) labeling, the medium was exchanged on day 7 for 15 ml of phosphate-free DMEM containing 0.1% bovine serum albumin (for experiments employing wortmannin, bovine serum albumin was omitted from the medium), after 6 h 1 mCi of P(i) was added, and cells were incubated overnight in the presence of the radioactive label before the various treatments were initiated. For preparation of extracts, the medium was removed from the plate, and the cells were washed twice with ice-cold buffer A (120 mM NaCl, 20 mM NaF, 10 mM pyrophosphate, 5 mM EGTA, 1 mM EDTA, 1 mM benzamidine, 0.1 mM phenylmethylsulfonyl fluoride, 30 mM 4-nitrophenyl phosphate, 50 mM Tris-HCl, pH 8.0) and then extracted in the same buffer containing 1% Nonidet P-40. Cell extracts were centrifuged at 12,000 g for 15 min at 4 °C, and the supernatant was immediately frozen in liquid N(2) and stored at -70 °C. Human embryonic kidney 293 cells were seeded at 10^6 cells/10-cm dish in DMEM supplemented with 10% (v/v) FCS. After 24 h, cells were transiently transfected with 10 µg of parent Myc-p70 DNA or mutant Myc-p70 DNA for 20 h employing a modified calcium phosphate precipitation method (27) . After transfection overnight, cells were washed twice with DMEM and arrested in G(O) for 24 h in DMEM. Cell extracts were prepared as above.

Quantitation of PIncorporation and Analysis of Phosphopeptides

Cell extracts were quickly thawed, the kinase was immunoprecipitated with M5 antiserum(28) , the precipitate was subjected to SDS-PAGE, and p70 was localized by autoradiography. After the P(i)-labeled p70 was excised from the gel, the amount of P(i) incorporated into the kinase was determined by Cerenkov counting, followed by its electroelution from the gel. Two-dimensional tryptic phosphopeptide analysis was performed as described previously (14) with the following modifications. The electroeluted protein was precipitated with 15% (w/v) trichloroacetic acid and performic acid oxidized prior to digestion(29) , and no endo-Lys C digestion was performed before the addition of trypsin (3 5 µg over 24 h). The phosphopeptides were separated on the same thin layer plates, but the cellulose used by the manufacturer (Merck) was changed, resulting in slower mobility of phosphopeptides in the chromotography dimension. Also, the resultant chromatograms were visualized using a PhosphorImager and ImageQuant Software (Molecular Dynamics) rather than long term autoradiography on film.

Immunoblotting

Equal amounts of total protein from extracts were subjected to SDS-PAGE and electrophoretically transferred to an Imobilon P membrane (Millipore), and the resultant membrane was incubated overnight with M1 antibody at 4 °C, with gentle agitation after blocking with 5% skim milk (Fluka)(9) . The blot was decorated with an anti-rabbit horseradish peroxidase-conjugated secondary antibody and developed using the ECL system (Amersham Corp.).

Protein Determination and in Vitro Kinase Assay

The protein concentration of cell extracts was measured using a Bio-Rad D/C protein assay kit with bovine serum albumin as the standard. For immunoprecipitation of p70, equal amounts (20 µg) of total protein from cell extracts were diluted in buffer A to a final volume of 200 µl, and then 5 µl of p70 M5 antibody was added. After incubation of the mixture on ice for 2 h, the immunocomplexes were incubated with protein A-Sepharose beads for 30 min at 4 °C with gentle shaking and then pelleted by centrifugation. The protein A immunocomplexes were washed four times in buffer A, washed one time with dilution buffer(30) , and resuspended in 5 µl of dilution buffer containing 1 mM dithiothreitol. The suspended protein A complexes were assayed for p70 activity as described previously(28) . Immunocomplex assays of the Myc epitope-tagged p70 were performed as above except that 9E10 anti-Myc antibody and protein G-Sepharose beads were used. For total S6 kinase activity, equal amounts of total protein from extracts were assayed directly without immunoprecipitation(28) . For MAP kinase assays, 150 µg of cell lysate was immunoprecipitated with 10 µl of a specific p42 polyclonal antibody. Immunoprecipitates were then assayed for MBP activity as described previously(31) . Total MAP kinase assays were carried out as above except without immunoprecipitation.


RESULTS

Autoinhibitory Domain

Recently, two mutant p70 constructs were described in which the four phosphorylation sites in the autoinhibitory domain, Ser, Ser, Thr, and Ser (Fig. 1A) were mutated to either acidic residues, (p70D(3)E), or neutral residues, (p70A(4))(15) . Transient expression of these mutants or the parent enzyme in human 293 cells demonstrated that p70D(3)E was as active as the parent construct, whereas the activity of p70A(4) was severely reduced(15) , consistent with these sites playing a major role in regulating p70 activity. However, if these are the only sites of phosphorylation associated with mitogen-induced kinase activation, then the activity of these two constructs should be mitogen-independent. To assess this possibility, p70, p70A(4), and p70D(3)E were each transiently expressed in human 293 cells, the cultures were arrested in G(O), and the S6 kinase activity of each construct was measured before and after serum stimulation. Even though the basal activity of p70D(3)E is higher than p70 and that of p70A(4) is lower, both constructs are activated by serum (Fig. 1B). Activation was associated in all three cases with a decrease in mobility of the kinase on SDS-PAGE (data not shown). These results indicate that kinase activation may be dependent on phosphorylation of other sites.


Figure 1: Multiple phosphorylation of p70 regulates its activity. A, schematic representation of p70 showing the location of the regulatory phosphorylation sites. AD represents the autoinhibitory domain. The model is drawn to scale(51) . B, the activity of parent Myc-p70(1) , Myc-p70A(4)(2) , or p70D(3)E (3) expressed in human 293 cells was determined in an immunocomplex assay before (stippledbars) and after (solidbars) stimulation with 10% FCS for 1 h. All activities were normalized for p70 expression levels by quantitation of Western blots probed with the 9E10 anti-Myc antibody, and decorated with rabbit anti-mouse IgG followed by I-conjugated protein A (Amersham Corp.). Quantitation of bound I-protein A was done using a PhosphorImager and ImageQuant software (Molecular Dynamics).



Serum-induced Phosphorylation Sites

Consistent with the above data, in initial phosphopeptide analysis studies other phosphopeptides were detected in some preparations(14) , and more recent studies have demonstrated that other phosphorylation sites play a critical role in regulating kinase activity(15) . To determine whether additional phosphorylation sites were associated with p70 activation, quiescent Swiss 3T3 cells were stimulated with serum for 15 min in the presence of P(i). Serum treatment leads to a rapid activation of p70 as measured by its ability to phosphorylate 40 S ribosomal protein S6 in vitro (Fig. 2A, lanes1 and 2) or its slower migration on Western blots of one-dimensional SDS-PAGE (Fig. 2A, lanes3 and 4). The shift in electrophoretic mobility corresponds to an increase in the incorporation of phosphate into the protein(13) . In quiescent cells, two forms of p70 can be clearly distinguished on Western blots, designated i and ii, which, upon serum stimulation, exhibit decreased mobility, designated iii and iv (Fig. 2A, compare lanes3 and 4). Analysis of two-dimensional tryptic phosphopeptide maps of p70 from quiescent cells revealed the presence of phosphate in Ser, Ser Thr, and Ser (Fig. 2B), the four sites residing in the autoinhibitory domain(14) . Serum stimulation leads to a rapid increase in the amount of P(i) incorporated into these four sites, and the appearance of three additional phosphopeptides (Fig. 2C), which have been shown to be singly phosphorylated at Thr, Thr, and Ser.^2 Thr is situated in the catalytic domain, whereas Thr and Ser are located in the linker domain, which couples the catalytic and autoinhibitory domains (Fig. 1A). The appearance of Thr, Thr, and Ser was unexpected, in that they were not detected in 3T3 cells in earlier studies(14) , but it is consistent with the results described in Fig. 1B and the observation that one or more of these sites appear to be involved in the mobility shifts detected in 293 cells transiently overexpressing p70(15) . In the initial study, the failure to detect these phosphopeptides in 3T3 cells was probably due to the fact that none of these sites reside in a predicted tryptic peptide,^2 suggesting the presence of a contaminating protease. Taken together the data demonstrate that all seven phosphorylation sites are rapidly induced upon mitogenic stimulation.


Figure 2: Serum-induced activation of p70 in Swiss 3T3 cells. A, p70 activity in extracts from quiescent cells (lane1) or cells stimulated with 10% FCS for 15 min (lane2) was assayed after immunoprecipitation with p70 M5 antibody as described under ``Experimental Procedures.'' Extracts (20 µg of total protein) from quiescent cells (lane3) or cells serum-stimulated for 15 min (lane4) were subjected to Western blot analysis. Derivatives i-iv represent increasingly phosphorylated forms of p70. Cells labeled with P(i) were incubated in the absence (B) or presence (C) of 10% FCS for 15 min. Immunoprecipitated p70 was subjected to two-dimensional tryptic phosphopeptide mapping following performic acid oxidation as described under ``Experimental Procedures.'' Identified phosphorylation sites of p70 are numbered according to the p70 sequence(51) , and the origin is indicated with an arrow.



Effect of Rapamycin

The three peptides phosphorylated at Thr, Thr, and Ser are rapidly dephosphorylated in response to rapamycin when p70 is transiently expressed in 293 cells(15) . To establish whether this is the case with endogenous p70 in 3T3 cells, quiescent cells were stimulated with serum in the presence of the inhibitory macrolide. As previously shown(10) , such treatment abolished p70 activation as measured by its ability to phosphorylate S6 in an immune complex assay (Fig. 3A, compare lanes1-3). However, in contrast to earlier findings(10) , phosphate continued to be incorporated into the protein, albeit at a much lower rate (Fig. 3B, compare lanes1-3). Analysis of the tryptic phosphopeptides derived from this material revealed that the incorporation of phosphate into Thr, Thr, and Ser was severely inhibited by rapamycin pretreatment, but surprisingly, the same was true for Ser (compare Fig. 3C with Fig. 2C), which was not sensitive to rapamycin when p70 was transiently expressed in 293 cells(15) . In contrast to the four rapamycin-sensitive sites, the amount of phosphate in Ser, Thr, and Ser continued to increase (compare Fig. 3C with Fig. 2C), consistent with the finding that rapamycin does not completely block the phosphorylation of the kinase (Fig. 3B). If, instead, rapamycin is added post-serum stimulation, the kinase is inactivated within 30 min (Fig. 3A) but is still highly phosphorylated (Fig. 3B). Tryptic phosphopeptide analysis reveals that the phosphorylation states of Ser, Thr, and Ser remain constant, whereas Thr and Ser are no longer visible, and Thr as well as Ser are reduced (compare Fig. 3D and Fig. 2C). These data indicate the possible involvement of two separate pathways in the activation of p70 by serum, only one of which is sensitive to rapamycin. Furthermore, the difference in phosphorylation of Ser following addition of rapamycin pre- and post-serum treatment, suggests that the phosphorylation on Ser may be dependent on Thr, Thr, and/or Ser.


Figure 3: Effect of rapamycin pretreatment or posttreatment on activation and phosphorylation of p70 in response to serum. Swiss 3T3 cells were labeled with P(i) and incubated in the absence (A and B, lane1) or the presence of 10% FCS (A and B, lane2) for 15 min or were preincubated for 30 min with 5 nM rapamycin and then stimulated with 10% FCS for 15 min (A and B, lane3, and C) or first stimulated with 10% FCS for 15 min and then treated with 5 nM rapamycin for 30 min (A and B, lane4, and D). A, total p70 activity was measured in an in vitro immunocomplex kinase assay as in Fig. 2. One unit of kinase incorporates 1 pmol of P(i)/min into S6. B, the amount of P(i) incorporated into p70 was quantitated by Cerenkov counting following immunoprecipitation of the kinase as described under ``Experimental Procedures.'' C and D, two-dimensional tryptic phosphopeptide maps of P(i)-labeled p70 from cells pretreated (C) or posttreated (D) with rapamycin was carried out as in Fig. 2.



Rescue by FK506

Inhibition of p70 activity by rapamycin has been shown to be blocked by an excess of FK506(10) , arguing that this effect is exerted through a complex with the immunophillin FKBP12. If the inhibitory effect of the rapamycin-FKBP12 complex is exerted through the dephosphorylation of Thr, Thr, and Ser as well as Ser, these sites should become rephosphorylated in the presence of FK506. As the S6 kinase activity of p70 in either immune complex assays or assays of total cell extracts is quantitatively equivalent(32) , all subsequent assays have been carried out in cell extracts. The results show that p70 activity in extracts from serum-stimulated cells that have been treated with rapamycin for 30 min can be rescued by the addition of an excess of FK506, consistent with the presence of mitogen-activated p70 kinase. FK506 alone has no effect on serum-induced p70 activity (Fig. 4A). In parallel, FK506 rescues the phosphorylation of Thr and Ser as well as Thr and Ser (compare Fig. 4, B and C) and, as expected, FK506 alone has little effect on the serum-induced tryptic-phosphopeptide pattern (Fig. 4D). Thus, reversal of the rapamycin inhibitory effect by FK506 is regulated through the same sites of phosphorylation.


Figure 4: Reversal of rapamycin-induced dephosphorylation of p70 by FK506. A, Swiss 3T3 cells were stimulated with 10% FCS for 30 min in the absence (A, lanes1 and 4, and D) or presence of 5 nM rapamycin (A, lanes2 and 3, B and C). Cultures were then washed and incubated for an additional 3 h in DMEM containing 10% FCS and either 5 µM FK506 (A, lanes3 and 4, C and D) or the carrier, 0.02% ethanol alone (A, lanes1 and 2, B). Whole cell lysates were subjected to S6 kinase assays as described previously under ``Experimental Procedures.'' B-D, two-dimensional tryptic phosphopeptide maps of p70 were analyzed from cells treated as in A, lanes2, 3, and 4, respectively, which had been prelabeled with P(i). Two-dimensional tryptic phosphopeptide analysis was carried out as in Fig. 2.



Effect of Wortmannin

The antibiotic wortmannin has been shown to specifically block activation of both phosphatidylinositol 3-OH kinase (18) and p70(17, 18) , though its specificity for the p70 pathway versus the p42/p44 pathway has been recently challenged(33, 34) . Furthermore, it has been hypothesized that the inhibitory effect is exerted upstream of the rapamycin block as TPA-induced activation of p70, presumably through protein kinase C, is insensitive to wortmannin but is still blocked by rapamycin(18) . However, wortmannin may also act on a rapamycin-independent signaling pathway, exerting its inhibitory effect on p70 through a rapamycin-insensitive phosphorylation site. To test the role of wortmannin, we first assessed its ability to induce inactivation of p70 in cells pre-treated with serum. Under these conditions, the IC for p70 inactivation is between 50 and 100 nM wortmannin (Fig. 5A, inset). In cells either pretreated with 200 nM wortmannin or treated with the same concentration of the antibiotic 15 min post-serum stimulation, p70 activation is either blocked or returns to basal levels within 30 min (Fig. 5A). The inhibitory effect on kinase activity is paralleled by an increase in the mobility of the kinase analyzed on Western blots of SDS-PAGE (Fig. 5B). To examine the effect of wortmannin on the phosphorylation pattern of p70, cells were either pretreated with wortmannin and then stimulated with serum or stimulated with serum followed by subsequent addition of the antibiotic. The results are very similar to those obtained with rapamycin (Fig. 3, C and D); wortmannin pretreatment severely suppressed the phosphorylation of Thr, Thr, Ser, and Ser, with phosphorylation proceeding in the rapamycin-insensitive sites, whereas treatment following serum stimulation leads to dephosphorylation of Thr and Ser with less of an effect on Thr and Ser (Fig. 5, C and D, respectively). These data support a model in which both agents are acting on the same signaling pathway.


Figure 5: Effect of wortmannin on p70. A (inset), Swiss 3T3 cells stimulated with 10% FCS for 15 min were then incubated with increasing concentrations of wortmannin for an additional 30 min. Cell extracts were prepared and assayed as described under ``Experimental Procedures.'' A and B, cells were preincubated for 30 min with 200 nM wortmannin and then stimulated with serum for 15 min (lane1) or first stimulated with 10% FCS for 15 min (lane2) and then treated with 200 nM wortmannin for 15 min (lane3) or 30 min (lane4). Cell extracts were prepared and either assayed for (A) S6 kinase activity or (B) the mobility of p70 on Western blots as described under ``Experimental Procedures.'' C, two-dimensional tryptic phosphopeptide maps of p70 from cells pretreated with wortmannin for 30 min prior to stimulation with 10% FCS for 15 min; D, maps of p70 from cells first stimulated with 10% FCS for 15 min followed by the addition of wortmannin for 30 min. Cells were prelabeled with P(i), and extracts were prepared as in A and B. Two-dimensional phosphopeptide analysis was carried out as described in Fig. 2.



Effects of SQ20006

Recent studies have shown that raising cAMP levels in T cells by applying forskolin, an adenylate cyclase agonist, together with IBMX, an inhibitor of cAMP-dependent phosphodiesterase, blocks p70 activation(21) . However, previous studies in Swiss 3T3 cells had shown that phosphodiesterase inhibitors alone, but not other agents that raise cAMP levels, are responsible for blocking mitogen-induced S6 phosphorylation, the target of p70(23) . The results in Fig. 6demonstrate that neither 8-bromo-cyclic AMP nor forskolin has an effect on serum-induced p70 activation, whereas IBMX had a small but significant inhibitory effect. In contrast, the methylxanthine SQ20006, a more potent inhibitor of phosphodiesterase and S6 phosphorylation(23, 35) , severely suppresses p70 activation. If SQ20006 is added post-serum stimulation, it also induces p70 inactivation as measured by its ability to phosphorylate S6 in vitro (Fig. 7A) or by its increased electrophoretic mobility on SDS-PAGE (Fig. 7B). Under these conditions SQ20006 has no effect on p42/p44 activation (Fig. 7A, inset). The effect on p70 activity and mobility shift suggests that SQ20006 is operating on the same phosphorylation sites as wortmannin and rapamycin. To examine this possibility the effect of SQ20006 on the pattern of p70 phosphorylation was analyzed. The rapamycin-sensitive sites of phosphorylation are also sensitive to SQ20006, exhibiting approximately the same qualitative pattern if added before or after serum stimulation (Fig. 7, C and D). These results support earlier conclusions that the inhibitory effect of phosphodiesterase inhibitors is not through raising cAMP levels but instead through blocking an upstream kinase (23) .


Figure 6: Effect of cAMP or phosphodiesterase inhibitors on p70 activity. Swiss 3T3 cells were preincubated for 30 min without (lane1) or with 500 µM 8-bromo-cAMP (lane2), 50 µM forskolin (lane3), 500 µM IBMX (lane4), or 1.2 mM SQ20006 (lane5) and then stimulated with 10% FCS for 15 min. Whole cell lysates were assayed for kinase activity as described under ``Experimental Procedures.''




Figure 7: Effect of SQ20006 on p70 activity and phosphorylation state. A, whole cell lysates from Swiss 3T3 cells, first stimulated with 10% FCS for 15 min and then treated with 1.2 mM SQ20006 for the indicated times were subjected to S6 kinase assays as described under ``Experimental Procedures.'' A (inset), Total MAP kinase activity from cell extracts of time point 0 and 30 min was done as described under ``Experimental Procedures.'' A, equal amounts of protein (16 µg) from cell extracts at indicated times were analyzed by Western blotting as described under ``Experimental Procedures.'' C and D, two-dimensional tryptic phosphopeptide maps of p70 from cells pretreated with 1.2 mM SQ20006 for 30 min prior to stimulation with 10% FCS for 15 min (C) or from cells first stimulated with 10% FCS for 15 min followed by the addition of 1.2 mM SQ20006 for 30 min (D). P(i)-labeled p70 was subjected to the two-dimensional phosphopeptide analysis as described in Fig. 2.



As wortmannin does not block TPA activation of p70, whereas rapamycin does, each agent has been argued to attack a unique target in the p70 signaling pathway. If both agents operate on the same pathway, the wortmannin target would be situated more proximal to the cell surface receptor, with the rapamycin target situated downstream. To determine where SQ20006 acts in this pathway, cells were stimulated with TPA in the presence or absence of all three agents. The results show that wortmannin has no effect on TPA activation of p70, as shown by others(17) , whereas rapamycin and SQ20006 block kinase activity (Fig. 8). In contrast to p70 activation, all three agents have no effect on p42 activation (Fig. 8). These results suggest that SQ20006 operates very similarly to rapamycin, possibly inhibiting a common target.


Figure 8: Effect of rapamycin, wortmannin, and SQ20006 on TPA activation of p70. Swiss 3T3 cells were incubated in the absence or presence of 0.5 µM TPA for 30 min following pretreatment for 30 min with either 1.2 mM SQ20006 (SQ), 200 nM wortmannin (WM), or 5 nM rapamycin (RAP). p70 and MAP kinase activity were measured following immunoprecipitation of equal amounts of protein from each cell extracts as described under ``Experimental Procedures.''




DISCUSSION

From the data presented here, it is evident that the phosphorylation of Thr, Thr, and Ser are largely responsible for the mobility shifts observed on Western blots following mitogenic stimulation. The most likely reason these sites were not detected in the initial analysis (14) was that all three reside in atypically cleaved tryptic peptides.^2 This problem is further compounded by the fact that the cleavage efficiency of these peptides is poor and varies between batches of trypsin. The ability of the marcrolide to induce an equivalent increase in mobility of the wild type p70 and the p70D(3)E mutant on SDS-PAGE (15) is consistent with the conclusion that the mobility shifts in p70 are due to the rapamycin-sensitive sites. Furthermore, the phosphorylation of the rapamycin-insensitive sites, like the rapamycin-sensitive sites, appears to have a large impact on kinase activity (Fig. 1). Thus, as has been described for other kinases (36) , only a subset of sites cause changes in electrophoretic mobility, and therefore, interpretations concerning the extent of p70 phosphorylation and activation by mobility shift should be treated cautiously.

Recently Chung et al.(17) , employing specific point mutants of the PDGF receptor, provided evidence for two separate signaling pathways leading to p70 activation. One pathway was regulated through tyrosines 740 and 751 and hypothesized to be mediated through activation of phosphatidylinositol 3-OH kinase, whereas the second pathway was regulated by phosphorylation of tyrosines 1009 and 1021, apparently signaling through protein lipase C. Interestingly, the phosphatidylinositol 3-OH kinase inhibitor wortmannin only blocked signaling from tyrosines 740 and 751 and not tyrosines 1009 and 1021, while rapamycin blocked p70 activation through both pathways(17) . These results led to the hypothesis that, in the pathway mediated by tyrosines 740 and 751, the rapamycin block lies downstream of the wortmannin block(18) . The hypothesis that both agents inhibit p70 activation through different components, which are located on the same signaling pathway, is consistent with their ability to block the same set of phosphorylation sites in the kinase (Figs. 3-5). Indeed, this same set of phosphorylation sites is also sensitive to SQ20006 treatment (Fig. 7).

Earlier studies had shown that phosphodiesterase inhibitors can inhibit or ablate serum-induced S6 phosphorylation(23, 37) . Recent results from Monfar et al.(21) employing the cAMP elevating agents forskolin and IBMX, demonstrated that the two agents together prevent interleukin 2-induced p70 activation in a T cell line, leading them to conclude that this inhibitory effect was exerted through raising intracellular levels of cAMP. The results presented here demonstrate that, in Swiss 3T3 cells, raising cAMP levels either by use of the nonhydrolyzable analogue 8-bromo-cAMP or an adenylate cyclase agonist has no effect on serum-induced p70 activation (Fig. 6), consistent with earlier studies on S6 phosphorylation(23) . However, IBMX alone had a 25% inhibitory effect on kinase activation, whereas the more potent phosphodiesterase inhibitor SQ20006 (35) had a more pronounced effect. Recent studies have shown that the structurally related 2-aminopurine analogue, olomoucine, selectively inhibits a number of cell cycle-regulated kinases both in vitro and in vivo(38) . Olomoucine however, had no effect on serum-induced p70 activation while blocking p42/p44 activation (data not shown), suggesting that SQ20006 or similar structural analogues may be useful tools in specifically analyzing the p70 signal transduction pathway.

SQ20006 has a very similar p70 inhibitory profile to that of rapamycin. However, earlier studies showed that SQ20006, at concentrations that completely inhibit p70 activation, ablate serum-induced up-regulation of protein synthesis(23) . In contrast, rapamycin only has a marginal effect on global protein synthesis(7) . Although having only a small effect on general protein synthesis, rapamycin selectively suppresses the translational up-regulation of a family of mRNA that are characterized by having a polypyrimidine tract at their 5` transcriptional start site(7) . Taken together this suggests that SQ20006 is inhibiting the function of at least one other cell component that is involved in the up-regulation of translation. Since the inhibitory effect of SQ20006 appears to be exerted at initiation(23) , this component may be one of the specific factors involved in initiation of translation(39) .

It is clear from the data presented here that the signaling events leading to p70 activation are not all converging through a rapamycin/wortmannin/SQ20006-sensitive pathway. Instead, there appear to be at least two independent pathways required for p70 activation, only one of which is blocked by the inhibitors employed here. The sites targeted by this pathway have been recently identified as Thr, Thr, and Ser

The complexity of p70 activation by phosphorylation is further emphasized by the observation that phosphorylation events mediated by these pathways may be interdependent. The results presented here raise the possibility that the phosphorylation of Thr and Ser may be dependent on phosphorylation of Thr and/or Ser (Figs. 3-6). Such an interpretation may also hold true for phosphorylation of Thr. This site is only found phosphorylated in the tryptic peptide Thr-Lys when Ser is also phosphorylated(14) . In contrast, the singly phosphorylated form of this peptide is only phosphorylated on Ser, implying that phosphorylation at the threonine residue is dependent on prior phosphorylation at the serine. Point mutations of individual sites, combined with phosphopeptide maps will help to resolve the importance of each site in hierarchal phosphorylation. Knowledge of the phosphorylation sites and their mutual interdependence will be an important tool in identifying the upstream kinases that regulate p70 activation.

Recent findings have indicated that p70/p85 plays a critical role in cell cycle progression(9, 10, 47) . Furthermore, it appears to mediate this effect through the phosphorylation of 40 S ribosomal protein S6 and the subsequent translational up-regulation of a family of mRNA transcripts that encode for components of the protein synthetic machinery(7, 8) . Up-regulation of specific translational components (48, 49) or obstruction of gene products that regulate their function (50) can transform cells or increase their susceptibility to transformation. These observations may explain why p70 is among the most highly conserved mammalian enzymes, having the identical sequence in man, mouse, rat, and rabbit. A highly regulated mechanism of p70 control would be consistent with the loss of this control leading to a constitutive growth state. The use of specific inhibitors should provide invaluable tools in probing regulatory pathways that govern p70/p85 activation and mechanisms that link the kinase to translational control.


FOOTNOTES

*
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
Recipient of a Human Frontier Science Program Organization long term postdoctoral fellowship.

Recipient of a European Molecular Biology Organization long term postdoctoral fellowship.

**
To whom correspondence should be addressed. Tel.: 0041 61 697 3012; Fax: 0041 61 697 6681.

(^1)
The abbreviations used are: PAGE, polyacrylamide gel electrophoresis; TPA, 12-O-tetradecanoylphorbol-13-acetate; IBMX, isobutylmethylxanthine; DMEM, Dulbecco's modified Eagle's medium; FCS, fetal calf serum; IgG, immunoglobulin G; FKBP12, FK506 binding protein 12; PDGF, platelet-derived growth factor; MAP, mitogen-activated protein.

(^2)
R. B. Pearson, P. B. Dennis, J.-W. Han, N. A. Williamson, S. C. Kozma, R. E. H. Wettenhall, and G. Thomas, submitted for publication.


ACKNOWLEDGEMENTS

We are indebted to Drs. B. A. Hemmings, H. B. J. Jefferies, S. C. Kozma, and A. Matus for critical reading of the manuscript. We also thank D. Schofield for her typing expertise and M. Rothnie for preparing the figures.


REFERENCES

  1. Kozma, S. C., and Thomas, G. (1994) Semin. Cancer Biol. 5,255-266 [Medline] [Order article via Infotrieve]
  2. Ferrari, S., and Thomas, G. (1994) CRC Crit. Rev. Biochem. Mol. Biol. 29,385-413
  3. Ballou, L. M., Luther, H., and Thomas, G. (1991) Nature 349,348-350 [CrossRef][Medline] [Order article via Infotrieve]
  4. Ming, X. F., Burgering, B. M. T., Wennström, S., Claesson-Welsh, L., Heldin, C. H., Bos, J. L., Kozma, S. C., and Thomas, G. (1994) Nature 371,426-429 [CrossRef][Medline] [Order article via Infotrieve]
  5. Reinhard, C., Fernandez, A., Lamb, N. J. C., and Thomas, G. (1994) EMBO J. 13,1557-1565 [Abstract]
  6. Stewart, M. J., and Thomas, G. (1994) BioEssays 16,1-7 [Medline] [Order article via Infotrieve]
  7. Jefferies, H. B. J., Reinhard, C., Kozma, S. C., and Thomas, G. (1994) Proc. Natl. Acad. Sci. U. S. A. 91,4441-4445 [Abstract]
  8. Jefferies, H. B. J., Thomas, G., and Thomas, G. (1994) J. Biol. Chem. 269,4367-4372 [Abstract/Free Full Text]
  9. Lane, H. A., Fernandez, A., Lamb, N. J. C., and Thomas, G. (1993) Nature 363,170-172 [CrossRef][Medline] [Order article via Infotrieve]
  10. Chung, J., Kuo, C. J., Crabtree, G. R., and Blenis, J. (1992) Cell 69,1227-1236 [Medline] [Order article via Infotrieve]
  11. Kuo, C. J., Chung, J., Fiorentino, D. F., Flanagan, W. M., Blenis, J., and Crabtree, G. R. (1992) Nature 358,70-73 [CrossRef][Medline] [Order article via Infotrieve]
  12. Price, D. J., Grove, J. R., Calvo, V., Avruch, J., and Bierer, B. E. (1992) Science 257,973-977 [Medline] [Order article via Infotrieve]
  13. Ballou, L. M., Jeno, P., and Thomas, G. (1988) J. Biol. Chem. 263,1188-1194 [Abstract/Free Full Text]
  14. Ferrari, S., Bannwarth, W., Morley, S. J., Totty, N. F., and Thomas, G. (1992) Proc. Natl. Acad. Sci. U. S. A. 89,7282-7285 [Abstract]
  15. Ferrari, S., Pearson, R. B., Siegmann, M., Kozma, S. C., and Thomas, G. (1993) J. Biol. Chem. 268,16091-16094 [Abstract/Free Full Text]
  16. Mukhopadhayay, N. K., Price, D. J., Kyriakis, J. M., Pelech, S. L., Sanghera, J., and Avruch, J. (1992) J. Biol. Chem. 267,3325-3335 [Abstract/Free Full Text]
  17. Chung, J., Grammer, T. C., Lemon, K. P., Kazlauskas, A., and Blenis, J. (1994) Nature 370,71-75 [CrossRef][Medline] [Order article via Infotrieve]
  18. Cheatham, B., Vlahos, C. J., Cheatham, L., Wang, L., Blenis, J., and Kahn, C. R. (1994) Mol. Cell. Biol. 14,4902-4911 [Abstract]
  19. Egan, S. E., and Weinberg, R. A. (1993) Nature 365,781-782 [CrossRef][Medline] [Order article via Infotrieve]
  20. Myers, M. G., Jr., Grammer, T. C., Wang, L. M., Sun, X. J., Pierce, J. H., Blenis, J., and White, M. F. (1994) J. Biol. Chem. 269,28783-28789 [Abstract/Free Full Text]
  21. Monfar, M., Lemon, K. P., Grammer, T. C., Cheatham, L., Chung, J., Vlahos, C. J., and Blenis, J. (1995) Mol. Cell. Biol. 15,326-337 [Abstract]
  22. Farrell, P. J., Balkow, K., Hunt, T., Jackson, R. J., and Trachsel, H. (1977) Cell 11,187-200 [Medline] [Order article via Infotrieve]
  23. Thomas, G., Siegmann, M., Kubler, A., Gordon, J., and Jimenez de Asua, L. (1980) Cell 19,1015-1023 [Medline] [Order article via Infotrieve]
  24. Evan, G. I., Lewis, G. K., Ramsay, G., and Bishop, J. M. (1985) Mol. Cell. Biol. 5,3610-3616 [Medline] [Order article via Infotrieve]
  25. Reinhard, C., Thomas, G., and Kozma, S. C. (1992) Proc. Natl. Acad. Sci. U. S. A. 89,4052-4056 [Abstract]
  26. &cjs2631;u&cjs2630;a, M., and Thomas, G. (1990) Proc. Natl. Acad. Sci. U. S. A. 87,7040-7044 [Abstract]
  27. Okayama, H., and Chen, C. A. (1988) BioTechniques 6,632-638 [Medline] [Order article via Infotrieve]
  28. Lane, H. A., Morley, S. J., Doree, M., Kozma, S. C., and Thomas, G. (1992) EMBO J. 11,1743-1749 [Abstract]
  29. Boyle, W. J., van der Geer, P., and Hunter, T. (1991) Methods Enzymol. 201,110-149 [Medline] [Order article via Infotrieve]
  30. &cjs2631;u&cjs2630;a, M., Vulevic, D., Lane, H. A., and Thomas, G. (1992) J. Biol. Chem. 267,6905-6909 [Abstract/Free Full Text]
  31. de Vries-Smits, A. M. M., Burgering, B. M. T., Leevers, S. J., Marshall, C. J., and Bos, J. L. (1992) Nature 357,602-604 [CrossRef][Medline] [Order article via Infotrieve]
  32. Chen, R., Chung, J., and Blenis, J. (1991) Mol. Cell. Biol. 11,1861-1867 [Medline] [Order article via Infotrieve]
  33. Welsh, G. I., Foulstone, E. J., Young, S. W., Tavare, S. W., and Proud, C. G. (1994) Biochem. J. 303,15-20 [Medline] [Order article via Infotrieve]
  34. Cross, D. A., Alessi, D. R., Vandenheede, J. R., McDowell, H. E., Hundal, H. S., and Cohen, P. (1994) Biochem. J. 303,21-26 [Medline] [Order article via Infotrieve]
  35. Chasin, M., Harris, D. N., Phillips, M. B., and Hess, S. M. (1972) Biochem. Pharmacol. 21,2443-2450 [CrossRef][Medline] [Order article via Infotrieve]
  36. Posada, J., and Cooper, J. A. (1992) Science 255,212-216 [Medline] [Order article via Infotrieve]
  37. Lastick, S. M., and McConkey, E. H. (1978) in Cell Reproduction: ICN-UCLA Symposia on Molecular and Cellular Biology (Dirksen, E. R., Prescot, D. M., and Fox, C. F., eds) Academic Press, Inc., New York _
  38. Vesely, J., Havlicek, L., Strnad, M., Blow, J. J., Donella-Deana, A., Pinna, L., Letham, D. S., Kato, J., Detivaud, L., Leclerc, S., and Meijer, L. (1994) Eur. J. Biochem. 224,771-786 [Abstract]
  39. Morley, S. J., and Thomas, G. (1993) in The International Encyclopedia of Pharmacology and Theraputics: Intracellular Messengers (Taylor, C. W., ed) pp. 447-483, Pergamon Press, New York
  40. Marshall, C. J. (1994) Nature 367,686 [CrossRef][Medline] [Order article via Infotrieve]
  41. Pearson, R. B., and Kemp, B. E. (1991) Methods Enzymol. 200A,62-81 [Medline] [Order article via Infotrieve]
  42. Thomas, G. (1992) Cell 68,3-6 [Medline] [Order article via Infotrieve]
  43. Blenis, J., Chung, J., Erikson, E., Alcorta, D. A., and Erikson, R. L. (1991) Cell Growth & Differ. 2,279-285
  44. Derijard, B., Hibi, M., Wu, I., Barrett, T., Su, B., Deng, T., Karin, M., and Davis, R. J. (1944) Cell 76,1025-1037
  45. Sanchez, I., Hughes, R. T., Mayer, B. J., Yee, K., Woodgett, J. R., Avruch, J., Kyriakis, J. M., and Zon, L. I. (1994) Nature 372,794-798 [Medline] [Order article via Infotrieve]
  46. Han, J., Lee, J.-D., Bibbs, L., and Ulevitch, R. J. (1994) Science 265,808-811 [Medline] [Order article via Infotrieve]
  47. Pierandrei-Amaldi, P., Beccari, E., Bozzoni, I., and Amaldi, F. (1985) Cell 42,317-323 [Medline] [Order article via Infotrieve]
  48. Lazaris-Karatzas, A., Montine, K. S., and Sonenberg, N. (1990) Nature 345,544-547 [CrossRef][Medline] [Order article via Infotrieve]
  49. Tatsuka, M., Mitsui, H., Wada, M., Nagata, A., Nojima, H., and Okayama, H. (1992) Nature 359,333-336 [CrossRef][Medline] [Order article via Infotrieve]
  50. Koromilas, A. E., Lazaris-Karatzas, A., and Sonenberg, N. (1992) EMBO J. 11,4153-4158 [Abstract]
  51. Kozma, S. C., Ferrari, S., Bassand, P., Siegmann, M., Totty, N., and Thomas, G. (1990) Proc. Natl. Acad. Sci. U. S. A. 87,7365-7369 [Abstract]

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