©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
Interleukin-1 and Endothelin Stimulate Distinct Species of Diglycerides That Differentially Regulate Protein Kinase C in Mesangial Cells (*)

(Received for publication, April 6, 1995; and in revised form, June 20, 1995)

Angela Musial Atashi Mandal Emmanuel Coroneos Mark Kester (§)

From the Departments of Medicine and Physiology/Biophysics, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
ACKNOWLEDGEMENTS
REFERENCES

ABSTRACT

Diglycerides are phospholipid-derived second messengers that serve as cofactors for protein kinase C activation. We have previously shown that, in rat glomerular mesangial cells, the cytokine, interleukin-1alpha, and the vasoactive peptide, endothelin, generate diglycerides from unique phospholipid precursors. However, neither the molecular species of these diglycerides nor their biological actions were determined. It is now hypothesized that interleukin-1- and endothelin-treated mesangial cells form distinct molecular species of diglycerides which may serve different roles as intracellular signaling molecules. Diglyceride molecular species were resolved and quantified by TLC and high performance liquid chromatography as diglyceride-[^14C]acetate derivatives. Endothelin stimulates predominantly ester-linked species (diacylglycerols) in contrast to interleukin-1 which stimulates only ether-linked species (alkyl,acyl- and alkenyl,acylglycerols). In support of these data, interleukin-1-treated mesangial cells hydrolyze ethanolamine plasmalogens, vinyl ether-linked phospholipids. It has been reported that ether-linked, in contrast to ester-linked, diglyceride species do not activate protein kinase C activity. Thus, we next assessed membrane protein kinase C activity in endothelin- or interleukin-1-treated mesangial cells. Even though interleukin-1 has no effect upon basal protein kinase C activity, this cytokine, through the formation of ether-linked diglyceride second messengers, inhibits endothelin, platelet-activating factor, or arginine vasopressin-stimulated protein kinase C activity. We further demonstrate that ester-linked diacylglycerols but not alkyl,acyl- or alkenyl,acylglycerols substitute for phorbol esters in a cell-free protein kinase C assay. In addition, alkenyl,acylglycerols inhibit diacylglycerol-stimulated immunoprecipitated protein kinase C alpha activity in vitro and total protein kinase C activity in permeabilized mesangial cells ex vivo. Taken together, these data suggest that interleukin-1-induced formation of ether-linked diglycerides may physiologically serve to down-regulate receptor-mediated protein kinase C activity and that individual molecular species of diglycerides may serve different roles as intracellular signaling molecules.


INTRODUCTION

Classical transmembrane signaling theory suggests that receptors are linked to a phosphatidylinositol 4,5-bisphosphate (PtdIns)(^1)-specific phospholipase C generating inositol phosphates and diglycerides (DG). However, distinct phospholipase C and phospholipase D activities that hydrolyze ester- and ether-linked phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEth) may also release unique molecular species of DG and phosphatidic acids. We have previously compared and contrasted the early phospholipid-derived second messengers generated by the vasoconstrictor, endothelin-1 (ET), and the cytokine, interleukin-1alpha (IL-1) in rat glomerular mesangial cells (MC). ET maintains the myogenic phenotype of these smooth muscle pericytes inducing MC contraction which leads to a decreased glomerular filtration rate through a diminished ultrafiltration coefficient(1) . IL-1 induces a phenotypic change in MC, switching these cells from a contractile to a secretory, immune-responsive, state with concomitant changes in matrix formation and adhesion protein expression, events associated with sclerosis(2) . ET generates DG initially from PtdIns and secondarily from PtdCho in MC(3) . Furthermore, ET-stimulated Ins-1,4,5-P(3) responses are transient, while elevated DG responses are sustained(4, 5, 6) . In contrast, IL-1 generates DG from PtdEth hydrolysis totally independent of any measurable polyphosphoinositide turnover or elevations in intracellular free calcium concentration ([Ca]) in MC(7) . Similar results demonstrating IL-1-induced DG derived from PtdCho hydrolysis (8, 9) or from an augmented sequential lysophosphatidic acid acyltransferase/phosphatidic acid phosphohydrolase activity (10) without concomitant Ins-1,4,5-P(3) generation have also been reported. This alternative signal transduction pathway which suggests that DG can be generated solely from sources besides the polyphosphoinositides has been demonstrated for diverse growth factors and cytokines including fibroblast growth factor(11, 12) , insulin (13) , EGF(14) , growth hormone(15) , interferon alpha(16) , D59(17) , Ha-ras(18) , IL-3(19) , and IL-1beta (20, 21) . Agonists that stimulate hydrolysis of phospholipids besides PtdIns may (interferon, IL-3(16, 19) ) or may not (IL-1, fibroblast growth factor(11, 20, 21, 22) ) stimulate PKC. IL-1, in all cell types studied, consistently does not induce PKC translocation or bioactivity. The role of the unique DG species generated by these agonists is still controversial as PtdCho-derived DG has been shown to be an effective (23) or ineffective activator of PKC(24, 25) . Agonists, such as IL-1, that generate DG independent of any polyphosphoinositide turnover have never been characterized in terms of their ester- and ether-linked sn-1 configuration. We hypothesize that ET and IL-1 generate discrete molecular species of DG that differentially regulate PKC.

As DG can be generated through distinct biochemical pathways and from different phospholipid precursors, it is very surprising that little information is currently available correlating agonist-induced DG structure with physiological function. Significant ether-linked DG species have been observed in PMA- and fMLP (formylmethionylleucylphenylalanine)-stimulated neutrophils (26) while only diacyl species have been observed with thrombin-stimulated I1 Cg cells (27) and IgG-stimulated mast cells(28) . The characterization of molecular species of DG from discrete phospholipid pools is especially important as ether-linked DG (alkyl,acyl- or alkenyl,acyl-DG species), in contrast to diacyl species, may inhibit PKC, not affect PKC (29, 30, 31, 32) , or only activate PKC in the presence of elevated levels(33) . Thus, cytokines such as IL-1 which generate DG at physiological levels of calcium may still be unable to activate PKC. In addition, ether-linked DG actually inhibit PKC activation induced by diacylglycerol species(30) . To date, there have been no studies that directly link agonist-stimulated ether-linked DG with an altered PKC activity.

Given the different signaling pathways that generate ET- and IL-1-induced second messengers in MC, our first aim was to characterize the molecular species of DG generated by these agonists as either diacyl-, alkyl,acyl-, or alkenyl,acylglycerol species. Our second aim was to correlate the formation of these individual molecular species of DG with PKC activation utilizing both cell-free and intact-cell methods.


MATERIALS AND METHODS

ET-1 was obtained from the Peptide Institute (Osaka, Japan) while IL-1 was purchased from U.B.I. (New York). Radiolabeled materials including [^3H]glycerol and [^14C]acetic anhydride were obtained from Amersham. DG and phospholipid standards were obtained from Deva Biologicals (Penn) or Serdary Biochemicals (London, Ontario, Canada). Polyclonal anti-PKC alpha antibody was obtained from Santa Cruz Biotechnology. All other materials were purchased from either Sigma or Calbiochem.

Glomerular Mesangial Cell Isolation and Culture

MC were grown from collagenase-digested glomeruli obtained from 100-g male Sprague-Dawley rats by a sequential sieving technique(34) . MC were grown in RPMI 1640 culture medium supplemented with 8.5% fetal bovine serum, 8.5% bovine calf serum, 100 units/ml penicillin, 100 µg/ml streptomycin, 5 µg/ml insulin, 5 µg/ml transferrin, and 5 ng/ml selenium at 37 °C in 5% CO(2). Cells were used in their 3rd to 15th passage. We have previously verified that the MC cultures are devoid of epithelial, endothelial, macrophage, and fibroblast contamination(34) .

Diglyceride Mass Analysis

DG extracted and chromatographically purified (3, 7, 34) from treated cells were acetylated with 1 µCi of [^14C]acetic anhydride and pyridine for 3 h at 37 °C, rechromatographed by TLC or HPLC(35) , and then subjected to autoradiography for identification of DG-acetate and subsequent liquid scintillation analysis. The DG-acetates (diacyl-, R = 0.465 ± 0.008; alkyl,acyl-, R = 0.585 ± 0.002; alkenyl,acyl-, R = 0.645 ± 0.005) are well-resolved from underivatized DG (R = 0.09 ± 0.01(f)) using a hexane/diethyl ether/methanol/acetic acid, 90:20:3:2 (v/v/v/v) elution system followed by drying the TLC plates and then a toluene (100, v) TLC solvent system run in the identical direction. The DG-acetate molecular species were also well-resolved by C18 HPLC using an acetonitrile, 2-propanol, methyl-5-butyl ether, H(2)O elution system, 83:10:5:2 (v/v/v/v). Derivatizing authentic DG species to DG-[^14C]acetates yielded products that comigrate with the corresponding DG-acetates prepared from phospholipid precursors. Incorporation of [^14C]acetate into DG was complete, linear with respect to DG mass, and showed no preference for ester- or ether-DG species and, thus, was used as a quantitative assay for DG mass. To demonstrate that the DG-acetate reaction was complete, we utilized [^3H]glycerol-labeled DG to follow [^14C]acetic anhydride incorporation and noted that no tritium label remained at the TLC position for underivatized DG. Calibration curves were constructed for each experiment and revealed a linear formation of DG-acetate from 0.05 to 100 µg of DG substrate utilized. Nonradioactive DG substrate was always used as an external standard after derivatization and was run on the outer and on the middle TLC lanes. DG-acetate extraction efficiency values were determined and were always greater than 80%(36) .

PKC

Membrane PKC activity was determined with the Amersham PKC enzyme assay system which utilizes the phosphorylation of an epidermal growth factor (EGF) receptor binding domain as a PKC-specific phosphorylation substrate. As modified by us, treated MC in Krebs Henseleit-HEPES were washed, and then 0.5 ml of buffer consisting of 50 mM Tris-HCl, 5 mM EDTA, 10 mM EGTA, 0.3% beta-mercaptoethanol, and 10 mM benzamidine was added to the MC monolayers in Petri dishes. The beta-mercaptoethanol buffer (pH 7.5) was supplemented with 50 µg/ml freshly prepared phenylmethylsulfonyl fluoride, 20 µg/ml leupeptin, and 20 µg/ml aprotinin. MC monolayers were then scraped and transferred to a precooled Dounce homogenizer and subjected to 20 pestle strokes on ice. Homogenized cells were centrifuged at 480 times g for 15 min at 4 °C to remove whole cells and nuclei and then ultracentrifuged at 70,000 times g for 30 min at 4 °C to pellet the membrane fraction. In selected experiments, PKC activity was assessed in the cytosol. The pellet was resuspended in 250 µl of beta-mercaptoethanol buffer plus 0.5% Triton X-100 by vortexing. 100 µl of the suspension was removed for Lowry protein analysis(37) . After 30 min, the resuspended pellet was centrifuged in an Eppendorf tube for 2 min at 4 °C and then the supernatant was removed for PKC analysis. Each experimental assay contained 25 µl of the membrane preparation or 25 µl of Triton beta-mercaptoethanol buffer control. Using the assay kit, experiments were run in the presence or absence of 12 mM calcium, 8 mol % phosphatidylserine (PtdSer), 24 µg/ml PMA, or 900 µM EGF receptor binding domain, and PKC activity was negligible when any of these reagents were omitted. Blank experiments which did not contain the membrane preparation were subtracted from all experiments. To start each assay, 10 µCi/ml Mg[P(i)]ATP (45 mM magnesium acetate plus 150 µM ATP) was added, and the reaction proceeded for 15 min at 25 °C. The reaction was stopped with 5% acetic acid, and solutions were transferred to phosphocellulose filters for washing and scintillation spectrophotometry counting. For calculations, 10 µl of the Mg[P(i)]ATP solution was counted by scintillation to quantify the specific radioactivity of the [P(i)]ATP solution. Data were expressed as picomoles of phosphate transferred per min per mg of protein.

In selected experiments, diradylglycerols with identical chain lengths and variable sn-1 linkages were substituted for PMA in the PKC assays and cell permeabilization experiments. The prepared diradylglycerols used in these studies all consisted of 16:0 and 18:1 hydrocarbon substituents in the sn-1- and sn-2-position, respectively. The concentration of our phospholipase C-derived DG species was determined from the initial concentration of phospholipid precursor. The phospholipase C reaction goes to completion and showed no preference for ether- or ester-linked phospholipids. Permeabilization of MC with 20 µg/ml saponin for 5 min was accomplished as described previously(38) . Efficiency of permeabilization was determined using [S]GTPS and [^3H]glycerol-labeled DG. This procedure did not elevate basal PKC activity or phospholipase C or D activities(38) . As should be expected, DG species did not affect membrane protein kinase C activity in nonpermeabilized cells.

In Vitro Reconstitution Assay for Immunoprecipitated PKC alpha

As adapted from previous studies(39, 40) , quiescent MC in 100-mm plates were washed twice with ice-cold phosphate-buffered saline supplemented with 1 mM vanadate and lysed on ice with 500 µl of a buffer containing 25 mM HEPES, pH 7.55, 100 mM NaCl, 20 mM beta-glycerophosphate, 1.5 mM MgCl(2), 1 mM vanadate, 0.5 mM EGTA, 0.25 mM EDTA, 0.1% Nonidet P-40, 1 mM phenylmethylsulfonyl fluoride, 10 µg/ml leupeptin, 10 µg/ml pepstatin, and 10 µg/ml aprotinin. 150 µg of cell lysate proteins were precleared with preimmune rabbit sera for 2 h at 4 °C. PKC alpha was immunoprecipitated from cleared lysates using 0.5 µg of polyclonal anti-PKC alpha (Santa Cruz Biotechnology). The formed immunocomplexes were subsequently collected with goat anti-rabbit IgG agarose after a 2-h incubation at 4 °C. The immunoprecipitated PKC alpha-agarose complex was washed twice and resuspended in a kinase reaction buffer containing 50 mM HEPES, pH 7.55, 25 mM beta-glycerophosphate, 75 mM KCl, 1 mM vanadate, 10 mM MgCl(2), and 0.1 mM CaCl(2). Phosphorylation assays were performed in 50 µl of kinase buffer in the presence of 1 µCi of [-P]ATP (10 mCi/mmol) per reaction and 20 µM unlabeled ATP, for 20 min at 30 °C. The reaction mixture (50 µl) also contained 10 µg of histone/reaction as exogenous substrate in the presence or absence of 40 µg/ml PtdSer/reaction and selected alkenyl,acyl- or diacylglycerols. The reactions were terminated with the addition of 25 µl of 3 times Laemmli buffer without reducing agent. Samples were heated for 5 min at 95 °C, and the phosphorylated proteins were resolved by 15% SDS-PAGE, visualized by autoradiography, and quantified by excising the bands. The incorporated radioactivity was determined by liquid scintillation spectrophotometry counting.

Plasmalogen Analyses

The vinyl ether bonds of plasmalogens are cleaved by acid hydrolysis. The released long chain fatty aldehydes react with 2,4-dinitrophenylhydrazine to form long chain fatty aldehyde-hydrazones which are measured spectrophotometrically at 390 nm. This reaction is specific for the vinyl ether bond of the plasmalogen since ester bonds cannot form aldehyde moieties. For determination of the free aldehyde concentration in each TLC spot, the acid hydrolysis step of the procedure is omitted. Phospholipids were extracted from MC as described before(34) . Each isolated phospholipid TLC spot may be composed of varying concentrations of 1,2-diacyl phospholipids and vinyl-ether-linked plasmalogens. Identical concentrations of phospholipid extracts were applied to each of two sets of chromatographs and assayed for either phospholipid phosphate (41) or plasmalogen content(42) . The percent composition of PtdEth and PtdCho in the MC monolayers was 17.4 ± 0.8% and 50.2 ± 5.9%, respectively. Total phospholipid mass did not change upon either a 1-min or 2-h IL-1 stimulation. Control preparations were found to contain 45.6 ± 1.1 nmol of ethanolamine plasmalogen/µmol of phosphatidylethanolamine and 9.4 ± 0.8 nmol of choline plasmalogen/µmol of phosphatidylcholine.

Statistical Analysis

After one-way or two-way analysis of variance (ANOVA) was used to establish differences among groups (p < 0.05), independent t tests were used to establish significant differences between groups. The p values of the individual comparisons were adjusted for multiple comparisons by the Bonferroni method.


RESULTS

Using [^14C]acetic anhydride, we have assessed diacyl-, alkyl,acyl-, and alkenyl,acylglycerol-[^14C]acetate species from vehicle- and ET- or IL-1-treated MC (Fig. 1). Previous experiments using [^3H]arachidonate (3, 7, 34) established 5 min as an optimal point to assess DG. These results, expressed as a mass measurement, indicated that ET generates predominantly ester-linked species (diacylglycerol) while IL-1 generates only ether-linked (alkyl,acyl- and alkenyl,acylglycerol) species. ET stimulate a 2.8-fold increase in diester-linked species in contrast to a nonsignificant 1.6-fold increase for ether-linked species. IL-1 treatment significantly increased alkyl,acylglycerol formation 2.2-fold and alkenyl,acylglycerol formation 2.8-fold. In data not shown, IL-1 concentrations as high as 30 ng/ml did not induce diacylglycerol formation. MC pretreated with IL-1 4 min before subsequent ET stimulation displayed an additive 5.0-fold increase in alkenyl,acylglycerol species formation which was not observed for the other species. Also, IL-1 treatment did not reduce ET-stimulated diacylglycerol formation. Expressed as ether/ester-DG percentages, control values of 54% were increased to 120% with IL-1 and reduced to 34% with ET. IL-1 restored this diminished ET-induced ether/ester-DG percentage to control levels of 56%. We next analyzed IL-1-stimulated (10 ng/ml) DG species at several time points that correspond to previously published data assessing IL-1-induced [^3H]DG formation(7) . As depicted in Fig. 2, IL-1 induced alkyl,acyl- and alkenyl,acyl- but not diacylglycerol at 1, 5, and 15 min. IL-1-stimulated ether-linked DG returned to baseline values by 30 min, confirming the earlier radioactive flux measurements (7) .


Figure 1: ET-stimulated MC generate predominantly ester-linked DG while IL-1-stimulated MC form only ether-linked DG species. MC were treated with ET and/or IL-1 for 5 min. DG mass was assessed as DG-[^14C]acetate for TLC-separated diacyl-, alkyl,acyl-, and alkenyl,acylglycerol species. n = 3 experiments, each experiment replicated in duplicate, mean ± S.E., *, p < 0.05.




Figure 2: IL-1-treated MC form ether- but not ester-linked DG as a function of time. DG mass was assessed as DG-[^14C]acetate for TLC-separated diacyl-, alkyl,acyl-, and alkenyl,acylglycerol species. n = 4 experiments, each experiment replicated in duplicate, mean ± S.E., p < 0.05 by one-way ANOVA for alkyl and alkenyl data.



We have utilized a spectrophotometric assay that ascertains the plasmalogen content (a vinyl ether-linked phospholipid) of TLC-separated phospholipids, to investigate if the elevation in IL-1-generated ether-linked DG corresponds to a decrease in ether-linked phospholipid species (Fig. 3). IL-1 (3 ng/ml)-treated MC responded with a decrease in ethanolamine plasmalogen but not choline plasmalogen mass which corresponds to the time course of IL-1-induced DG generation(7) . In addition, these studies confirmed our earlier studies (7) that, in MC, IL-1 receptor signaling is linked to hydrolysis of ethanolamine- but not choline-containing phospholipid species.


Figure 3: IL-1-treated MC hydrolyze ethanolamine but not choline plasmalogens. Plasmalogen (alkenylphospholipid) content was assessed spectrophotometrically by measuring long chain fatty aldehydes released from the sn-1-position of the phospholipid. n = 4, mean ± S.E., p < 0.01 by two-way ANOVA.



As it has been reported that ether-linked diglyceride species, in contrast to diacylglycerol species, do not activate PKC activity, we treated MC with either IL-1 which generates only ether-linked species or ET which generates predominantly diacyl species and assessed PKC activity (Fig. 4). Using this Triton X-100 mixed micelle EGF-receptor binding domain phosphorylation assay system (Amersham) and a partially purified MC membrane preparation, we have previously demonstrated that PMA activates PKC activity in the presence but not the absence of exogenous PtdSer, calcium, or membrane preparation. ET (100 nM) stimulated membrane PKC activity compared to vehicle- or IL-1 (3 ng/ml)-treated MC. The doses selected elicited maximal DG stimulation in MC. In data not shown, IL-1 concentrations as high as 100 ng/ml did not stimulate membrane PKC activity. Moreover, in data not shown, in a complementary manner, ET reduced cytosolic PKC activity. At 5 min, membrane PKC activity was 32, 29, and 71% of total PKC activity for control, IL-1-, and ET-stimulated MC, respectively, suggesting that ET but not IL-1 translocates PKC activity to the membrane. These studies which demonstrate that IL-1 does not stimulate PKC activity or translocation as assessed by phosphorylation of an EGF-receptor binding domain have been confirmed using a PKC pseudosubstrate (Life Technologies, Inc.) or histone as alternative substrates.


Figure 4: ET but not IL-1 stimulates membrane PKC activity in MC. For these experiments, ionomycin, IL-1, and/or ET were added to MC monolayers, membranes were prepared and assayed for PKC activity in the presence of all cofactors including calcium. n = 7 to 30 experiments, each experiment replicated in triplicate or quadruplicate, mean ± S.E., *, p < 0.05.



We have shown previously that IL-1 does not stimulate inositol trisphosphate production or induce elevations in intracellular free calcium concentrations(7) . Thus, we used ionomycin (a calcium ionophore) to elevate intracellular calcium concentration to levels observed with 100 nM ET stimulation and then assessed the effects of IL-1 upon membrane PKC activity (Fig. 4). Ionomycin induced a slight increase in PKC activity that was not potentiated with IL-1 suggesting that IL-1 does not activate PKC in the presence or absence of elevation of intracellular calcium. To test if IL-1-generated ether-linked DG have an inhibitory effect upon receptor-activated PKC activity, we pretreated MC with IL-1 or vehicle for 5 min and then assessed ET-stimulated PKC activity for an additional 15 min (Fig. 4). IL-1 had no effect on basal PKC activity but did reduce ET-stimulated PKC activity, suggesting a physiological inhibitory role for IL-1-generated ether-linked DG. In data not shown, this inhibitory effect of IL-1 upon ET-stimulated PKC activity was evident even without preincubation. In addition, IL-1 also inhibited platelet-activating factor- and arginine vasopressin-stimulated PKC activity. ET (100 nM) stimulated membrane PKC activity in a time-dependent manner (Fig. 5), and we chose either a 5-min or 15-min ET stimulation for all subsequent experiments, as these time points correspond to maximal ET-stimulated DG formation and PKC activation. At all time points studied, IL-1 (3 ng/ml) did not stimulate PKC activity above control values.


Figure 5: ET (10M) stimulates membrane PKC activity in a time-dependent manner. Total PKC activity was assessed by phosphorylation of the EGF-receptor binding domain peptide as an exogenous substrate. n = 3 experiments, each replicated in triplicate, mean ± S.E., *, p < 0.05, one-way ANOVA.



To test whether ET-induced diacylglycerols but not IL-1-generated ether-linked diglycerols stimulate PKC, we next substituted diacyl-, alkyl,acyl-, and alkenyl,acylglycerol species for PMA in the cell-free PKC assay system (Fig. 6). Substituting diacylglycerol but not alkyl,acyl- and alkenyl,acylglycerols for PMA in the PKC assay elevated basal membrane PKC activity. PtdSer stimulated PKC activity in the absence of PMA and served as baseline control. The mol % of PtdSer and DG have previously been shown to be optimal for this assay(34) . To confirm and extend these cell-free studies that assess total PKC activity, we next specifically evaluated immunoprecipitated PKC alpha in an in vitro kinase assay (Fig. 7). MC express PKC alpha which is acutely activated by G-protein-linked receptors (43) . (^2)A 4-min preincubation with 10 nM alkenyl,acylglycerol (AAG) diminished 100 nM diacylglycerol (DAG)-stimulated, immunoprecipitated, PKC alpha activity as assessed by histone phosphorylation. Actual counts/min/lane values for the excised histone bands were, respectively, 3507, 200, and 173 for diacylglycerol-, alkenyl,acylglycerol-, and alkenyl,acylglycerol/diacylglycerol-treated immunoprecipitates. In addition, alkenyl,acylglycerol inhibited diacylglycerol-induced autophosphorylation of PKC alpha. These data suggest that IL-1- and ET-receptors increase the content of distinct species of DG that differentially activate PKC in MC.


Figure 6: Substituting diacylglycerols but not alkyl,acylglycerols or alkenyl,acylglycerols for PMA in the cell-free PKC assay elevates PKC activity. Membranes prepared from control MC monolayers were assayed for PKC activity in the presence of PtdSer, calcium, and selected DG or PMA. n = 7 experiments, each experiment replicated in quadruplicate, mean ± S.E., *, p < 0.05.




Figure 7: Alkenyl,acylglycerol inhibits diacylglycerol activation of PKC alpha as assessed by [P]PO(4) incorporation into histone. Immunoprecipitated PKC alpha activity was measured in an in vitro kinase reaction that contained PtdSer, calcium, and either diacyl (DAG)- or alkenyl,acylglycerol (AAG) species. For visual clarity, the autoradiogram exposure time was 6 h for the diacylglycerol lane and 72 h for the AAG and AAG/diacylglycerol lanes. A representative autoradiogram of two such experiments.



To definitively assess the effects of DG molecular species upon PKC activity, we incubated permeabilized MC monolayers (38) for 10 min with 100 nM diacyl-, alkyl,acyl-, or alkenyl,acylglycerol species or vehicle and then assessed membrane PKC activity (Fig. 8). These intact cell experiments are the in vivo equivalents of the cell-free experiments that substitute DG species for PMA in the assay itself. Using the permeabilized cells, diacylglycerol but not ether-linked glyceride species stimulated PKC activity. Ether-linked DG concentrations as high as 1 µM did not stimulate PKC activity (data not shown). Also, in data not shown, maximal concentration of ET (100 nM) and diacylglycerols (100 nM) did not have a synergistic effect upon PKC activity, suggesting activation through a common pathway.


Figure 8: Exogenous application of diacylglycerol species but not alkyl,acylglycerol or alkenyl,acylglycerol species to permeabilized MC stimulate membrane PKC activity. Saponin-treated MC were incubated with 100 nM concentrations of the individual DG species for 10 min and then homogenized for PKC analysis. In nonpermeabilized cells, 100 nM diacylglycerol-induced PKC activity was only 6.0% above corresponding control. n = 6 experiments, each experiment replicated in quadruplicate, mean ± S.E., *, p < 0.05.



We next tested if alkenyl,acylglycerol could inhibit diacylglycerol-induced PKC activation in this intact cell model. Alkenyl,acylglycerol species (1 nM through 1 µM) were added 4 min before diacylglycerol species (100 nM) and then membrane PKC was assessed after 10 min. (Fig. 9). Similar results were obtained if the alkenyl,acylglycerol species were added simultaneously with the diacylglycerol species (data not shown). Alkenyl,acylglycerol inhibited diacylglycerol-induced PKC activity in a dose-dependent manner. Specifically, 1 nM alkenyl,acylglycerol reduced 62% of membrane PKC activity induced by 0.1 µM diacylglycerol. These data suggest, but do not prove, that ether-linked DG species compete for the diacylglycerol cofactor site on PKC without activating the enzyme.


Figure 9: Exogenous application of alkenyl,acylglycerols inhibit stimulation of membrane PKC activity induced by diacylglycerols in permeabilized MC. Saponin-treated MC were incubated with various doses of alkenyl,acylglycerol for 4 min before addition of 100 nM diacylglycerol for another 6 min. MC membranes were then prepared and assayed for PKC activity. n = 6 experiments, each experiment replicated in quadruplicate, mean ± S.E., *, p < 0.05.




DISCUSSION

Vasoconstrictors, cytokines, and growth factors stimulate the formation of DG second messengers, yet each agonist leads to separate physiological end points. To further define the biological actions of ET-1 (vasoconstriction and proliferation) and IL-1 (inducibility of genes associated with matrix formation and synthesis of other proinflammatory mediators), we investigated the early signal transduction pathways for these agonists with particular emphasis on the distinct molecular species of DG generated and their potential regulation of PKC activity. The biochemical information contained in the structure of these unique DG species may determine, in part, the phenotypic changes induced by each of these agonist groups. Solely on the basis of the sn-1 bond linkage of the DG, we have demonstrated that ET and IL-1 generate distinct DG species that differentially regulate PKC. IL-1-treated MC generated only ether-linked DG species in contrast to ET-1-stimulated MC which formed predominantly ester-linked (diacyl) DG species. Furthermore, ET but not IL-1 stimulated basal membrane PKC activity, while IL-1 decreased receptor-stimulated PKC activity. Our results further suggest that it is the elevation of vinyl ether-linked DG species and not a corresponding decrease in diacylglycerols that leads to IL-1 receptor-linked reduction of ET-activated PKC. The action of IL-1-induced ether-linked DG to inhibit ET-stimulated ester-linked DG was shown with both immunoprecipitated PKC alpha in a cell-free system and with total PKC in a permeabilized cell protocol. Thus, the physiological role of IL-1-induced formation of ether-linked DG may be to down-regulate receptor-mediated PKC activation.

We demonstrate that IL-1 inhibits receptor-stimulated PKC activity by generating ether-linked DG which have been suggested to competitively bind to PKC without activating PKC(44) . Alternatively, IL-1 pretreatment of ET-stimulated MC maintains ether/ester-DG percentages at levels that do not favor PKC activation. It has been suggested that the carboxyl groups of the ester bond at the sn-1-position as well as the resulting bond angle are essential to PKC activation (45, 46) . It follows that it may be the characteristic kink in the sn-1 ether bond of alkyl- or alkenylphospholipids which allows IL-1 receptors to activate a phospholipase that selectively hydrolyzes these substrates. It is now widely believed that a physical property of DG to lower the binding energy for membrane/PKC interactions favors translocation of the kinase to the membrane(44) . Thus, as an alternative mechanism of action, ether-linked DG may not increase the apparent membrane binding constant for PKC. Recent studies have suggested that it is not PKC activation or translocation that is ultimately responsible for an altered phenotype, but, in fact, it is down-regulation and/or proteolysis of the activated PKC isotypes(47) . This theory might explain the observation that chronic but not acute phorbol ester treatment is a cell proliferation signal(48) . If down-regulation of a specific isotype is critical for activation of a specific cellular phenotype, then a more direct mechanism, competitive inhibition of selected PKC isotypes with ether-linked DG, might also lead to an altered cellular phenotype.

In terms of mass, there is a discrepancy between IL-1-induced hydrolysis of ethanolamine plasmalogen and formation of alkenyl,acylglycerol. Nearly 20 pmol of plasmalogen are hydrolyzed while only 1 pmol of alkenyl,acylglycerol is formed. This observation may reflect the fact that IL-1 might activate alkenylphospholipid-selective phospholipases, including A(2) and D which would degrade plasmalogen without directly forming alkenyl-DG species(49, 50) . Vinyl ether-selective phospholipase A(1) (plasmalogenase) and lysophospholipase A(1) (lysoplasmalogenase) activities have also been described which may be regulated by IL-1-receptors(51, 52) . Supporting our data, this phospholipase A(1) activity is specific for ethanolamine but not choline plasmalogens(51) .

In MC, PKC isoenzymes alpha, , , and are expressed, and alpha, , but not can be down-regulated by chronic PMA treatment (43) . The role of IL-1-induced ether-linked DG to inhibit vasoconstrictor-stimulated PKC alpha is supported by the following observations: 1) IL-1 inhibits ET-, arginine vasopressin-, and platelet-activating factor-stimulated PKC activity; 2) all of these G protein-linked vasoconstrictors activate PKC alpha; and 3) ether-linked DG inhibit immunoprecipitate PKC alpha in an in vitro assay. The putative actions of IL-1-stimulated ether-linked DG to negatively regulate calcium-insensitive PKC isotypes awaits further investigation. Even though IL does not stimulate PKC translocation or activation, it could be that IL-1 induces activation of PKC which is not regulated by DG cofactors. It is for this reason we have used the EGF-receptor binding domain to assess PKC activation as this substrate, and not histones, is phosphorylated in vitro by PKC (53, 54) . Moreover, we confirmed our findings by utilizing the alanine to serine mutated pseudosubstrate peptide sequence as a PKC substrate(55) .

The DG cofactor substrate specificity for individual PKC isotypes has only recently been investigated as a potential signaling mechanism. In a recent report, supplementation of 1-O-hexadecylglycerol into fibroblasts of Zellweger patients leads to accumulation of ether-linked DG and a concomitant inhibition of bradykinin-induced translocation of PKC alpha but not or (56) . Our studies extend these findings by demonstrating that IL-1-induced ether-linked DG inhibit immunoprecipitated PKC alpha activity in an in vitro assay. In other studies, PtdIns-derived DG preferentially containing arachidonate at the sn-2-position are a better cofactor for PKC alpha compared with PKC beta or , in contrast to PtdCho-derived DG species which are equipotent in stimulating PKC isoenzymes(57) . Also, the mitogenic effects of insulin have been linked to PtdCho-dependent phospholipase C activity that generates undefined species of DG that specifically activates PKC (58) . In a scenario similar to IL-1-receptor signaling in MC, interferon which does not elevate [Ca] and forms PtdCho-derived DG, translocates PKC but not alpha in Daudi cells(59) . Alternatively, a single agonist may have differential effects on multiple PKC isotypes as retinoic acid-induced differentiation is associated with PKC inhibition (60) and PKC alpha stimulation(61) . Thus, signaling mechanisms will be defined in the future by the type of PKC isotypes mobilized as well as the DG cofactor specificity for that PKC isotype.

Our studies are the first report of IL-1 inhibiting receptor-stimulated PKC activity. However, this is not the first instance of IL-1 negatively modulating the actions of vasoconstrictors. IL-1 pretreatment inhibited phenylephrine-induced contraction in rat aortic rings (62) and inhibited cerebral spinal fluid-contracted pial arteries (63) . Our data suggest that one component of IL-1alpha signaling is ether-linked DG inhibition of PKC activity. Other signaling pathways that have been postulated include nuclear targeting of a 16-kDa N-terminal IL-1alpha cleavage product that may function as a trans-activating factor(64) . Alternatively, the carboxyl end of IL-1alpha bound to the IL-1 receptor also localizes to the nucleus (65) . The role of JAK/STAT kinases to amplify early IL-1 receptor signals is an area of active investigation. In addition to inhibiting PKC activity with subsequent effects upon contraction and/or differentiation, ether-linked DG may exert other effects independent of PKC. Ether-linked phospholipids are preferentially esterified at the sn-2-position with arachidonate (66) and, thus, these DG may be a potential substrate for arachidonate release via a DG-lipase. IL-1 has been shown to induce prostanoids in various cell types(67) , and the contribution of the DG-lipase mechanism to arachidonate release has not been investigated. Other PKC-independent actions of DG include leukocyte chemoattraction(68) , adipocyte glucose transport(69) , neuronal calcium current regulation(70) , K-induced calcium influx regulation(71) , and translocation of phosphorylcholine cytidyltransferase activity(72) . DG may serve as a cofactor for other kinases besides PKC including c-Raf and PAK-2(73) . Finally, other signaling cascades may augment ether-linked DG-induced inhibition of PKC. For example, IL-1 and PDGF have recently been shown to stimulate sphingomyelin metabolism(74, 75) . However, IL-1, in contrast to PDGF, does not stimulate ceramidase activity to form the endogenous PKC inhibitor, sphingosine(76) .

Our studies suggest that IL-1 and ET stimulate distinct species of DG that differentially regulate PKC in MC. This is the first study to conclusively link agonist-stimulated ether-linked DG formation with a physiologically relevant function; i.e. inhibition of PKC activity. An alternative signal transduction pathway is envisioned by which agonists, such as IL-1, that hydrolyze PtdCho and PtdEth but not PtdIns predominantly form ether- but not ester-linked DG species. Our conclusions lead to the provocative speculation that individual molecular species of DG may serve as key metabolic branch points, coupling discrete receptor populations to specific PKC isotypes.


FOOTNOTES

*
This work was supported by National Institutes of Health Grant AR40225. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked ``advertisement'' in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§
To whom correspondence should be addressed: Dept. of Medicine, Case Western Reserve University, 10900 Euclid Ave., Cleveland, OH 44106. Tel.: 216-368-1246; Fax: 216-368-1249.

(^1)
The abbreviations used are: PtdIns, phosphatidylinositol; PtdCho, phosphatidylcholine; PtdEth, phosphatidylethanolamine; PtdIns 4,5-P(2), phosphatidylinositol 4,5-bisphosphate; PtdSer, phosphatidylserine; ET, endothelin-1; IL-1, interleukin-1alpha; PKC, protein kinase C; PMA, phorbol 12-myristate 13-acetate; R, migration relative to solvent front (variability in the center of the spot); DG, diglyceride(s); diacylglycerol, 1,2-diacyl-sn-glycerol; alkyl,acylglycerol, 1-O-alkyl-2-acyl-sn-glycerol; alkenyl,acylglycerol, 1-O-alk-1`-enyl-2-acyl-sn-glycerol; [Ca], cytosolic free calcium concentration; Ins 1,4,5-P(3), inositol 1,4,5-trisphosphate; MC, rat glomerular mesangial cell(s); EGF, epidermal growth factor; PKC, protein kinase C; HPLC, high performance liquid chromatography; GTPS, guanosine 5`-3-O-(thio)triphosphate; ANOVA, analysis of variance.

(^2)
A. Musial, A. Mandal, E. Coroneos, and M. Kester, unpublished data.


ACKNOWLEDGEMENTS

We thank Norma Minear for preparing the manuscript.


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