Sezione di Microbiologia, DMCSS, University of Bologna, Policlinico S.Orsola-Malpighi, via Massarenti 9, 40138, Bologna, Italy
Received 2 January 2002; returned 21 March 2002; revised 30 May 2002; accepted 13 June 2002
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Abstract |
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Introduction |
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Garenoxacin is a des-F(6) quinolone (lacking the 6-position fluorine typical of existing fluoroquinolones) with a broad range of antibacterial activity against Gram-negative and Gram-positive pathogens, including certain quinolone-resistant strains.4 This des-F(6) quinolone exhibits activity against respiratory pathogens and could be a therapeutic option for empirical treatment of respiratory tract infections. The high intrinsic in vitro activity against fastidious and atypical microbial species such as mycoplasmas3 or ureaplasmas, Legionella spp., Helicobacter pylori,5 Borrelia burgdorferi, C. trachomatis and C. pneumoniae strains and gonococci,6 supports its use for the treatment of community and nosocomial infections.
Garenoxacin has the same mechanism of action against DNA topoisomerases as other quinolones but has a stronger level of potency.6 In fact, its antibacterial activity and spectrum compared with five quinolones (trovafloxacin, moxifloxacin, levofloxacin, ofloxacin and ciprofloxacin) was found to be high against a significant number of Gram-positive and Gram-negative, aerobic and anaerobic bacteria.6
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Materials and methods |
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A total number of 33 Chlamydia isolates were tested: 10 C. trachomatis isolates, three C. pneumoniae isolates and 20 C. psittaci isolates. Isolates of C. trachomatis included five typed strains (serovars D, E, H, I and LGV2) and five untyped clinical isolates recently isolated from urethral swabs of male patients with non-gonococcal urethritis. C. pneumoniae isolates comprised one recent isolate from Italy and two reference isolates: IOL-207 (originally isolated in 1967 in London from an Iranian child with trachoma) and CM-1 (a clinical isolate from the Centers for Disease Control and Prevention, Atlanta, GA, USA, ATCC VR-1360) serologically identical by the micro-immunofluorescence test to the reference isolate TW183 (ATCC VR-2282). The 20 isolates of C. psittaci studied included eight reference strains and 12 isolates from infected animals in Italy (provided by Dr Magnino, Diagnostic Section, Zooprophylactic Institute, Pavia, Italy). The eight reference strains were represented by the avian (parakeet) strain 6BC (USA), the ovine (lamb) strain A22 (Scotland), the ovine strains W73, S26/3 and P787 (Scotland), the caprine abortion strain Krauss 15 (Scotland), the feline pneumonitis strain FePn-145 (USA) and the meningopneumonitis strain MePn-343 (ferret, USA). Susceptibility testing of the 33 chlamydial isolates tested was performed in LLC-MK2 cells (a continuous cell line derived from Rhesus monkey kidney tissue) grown in 24-well plates containing a glass coverslip (diameter, 12 mm) at the bottom. The cell growth medium was Eagles minimum essential medium (EMEM) supplemented with 10% heat inactivated fetal calf serum (FCS), 10 mg/L gentamicin, 10 mg/L vancomycin and containing 2 mM glutamine and 1.7 mg/L glucose.
Antimicrobial drugs and sensitivity assays
The antimicrobial drugs were provided as powders and solubilized according to the instructions of the manufacturers. Garenoxacin was supplied by Bristol-Myers Squibb (Syracuse, NY, USA), levofloxacin was purchased from Ortho-McNeil Pharmaceutical (Mt Prospect, IL, USA), ciprofloxacin was supplied by Bayer (West Haven, CT, USA), and doxycycline, roxithromycin and erythromycin were purchased from Sigma (Milan, Italy). Each of the 24-well plates was inoculated with an inoculum of chlamydiae that yielded 5 x 103 inclusion forming units (ifu) per millilitre and centrifuged at 1700g for 1 h. The medium was then removed and replaced with EMEM supplemented with 10% FCS, glucose (5 mg/L), cycloheximide (1 mg/L) and serial two-fold dilutions of each antibiotic. All tests were run in triplicate. After incubation at 35°C for 48 h, cultures were fixed with methanol and stained for inclusions with a fluorescein-conjugated monoclonal antibody specific for the chlamydial lipopolysaccharide genus-specific antigen.7 The MIC was the lowest concentration preventing >90% chlamydial inclusion detection compared with the drug-free control. The minimal bactericidal concentration (MBC) was determined by aspirating the antibiotic-containing medium, washing wells twice with phosphate-buffered saline, adding antimicrobial-free medium and re-incubating the plates for 48 h at 35°C. Cells were then fixed and stained as described above. The MBC was the lowest antibiotic concentration resulting in >90% reduction of inclusions.
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Results |
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Discussion |
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In this study, the activity of the new quinolone, garenoxacin, has been tested in vitro against Chlamydia spp. in comparison with other quinolones, and with doxycycline and the macrolide drugs, erythromycin and roxithromycin.
Garenoxacin was the most active of the quinolone drugs tested, with MICs and MBCs ranging from 0.007 to 0.03 mg/L. The concentration of garenoxacin at which 90% of the isolates were inhibited and killed was 0.03 mg/L for C. trachomatis and C. psittaci isolates. The MICs and the MBCs of garenoxacin ranged from 0.007 to 0.015 mg/L for the three C. pneumoniae isolates. The MICs and MBCs of garenoxacin were consistent from species to species and from strain to strain. These results are very similar to those reported recently by Malay et al.8 who tested five strains of C. trachomatis (MICs and MBCs of 0.015 mg/L) and 20 recent clinical isolates of C. pneumoniae (MIC90 and MBC90 of 0.015 mg/L).
The finding that for the 33 chlamydial isolates studied the MICs of garenoxacin, levofloxacin and ciprofloxacin were the same as the MBCs confirms previous observations that quinolones, at concentrations equivalent to their MICs, seem to kill chlamydial strains.9,10 Our MIC data were also similar to a previous finding.10 Malay et al.8 reported that garenoxacin was the most active quinolone against C. pneumoniae and C. trachomatis isolates tested. Our data confirm those results. In addition, our data also demonstrated that garenoxacin was the most active quinolone against C. psittaci, therefore demonstrating that garenoxacin is the most active quinolone tested thus far against chlamydiae.
Finally, it is noteworthy that in this study there was no significant variation in antimicrobial sensitivity among strains from the various chlamydial species and several passages of the chlamydial strains in cell culture did not modify their sensitivity to antimicrobial drugs.
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Footnotes |
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References |
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2 . Woodcock, J. M., Andrews, J. M., Boswell, F. J., Brenwald, N. P. & Wise, R. (1997). In vitro activity of BAY 12-8039, a new fluoroquinolone. Antimicrobial Agents and Chemotherapy 41, 1016.[Abstract]
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Takahata, M., Shimakura M., Hori, R., Kizawa, K., Todo, Y., Minami, S. et al. (2001). In vitro and in vivo efficacies of T-3811ME (BMS-284756) against Mycoplasma pneumoniae. Antimicrobial Agents and Chemotherapy 45, 3125.
4 . Jones, R. N., Pfaller, M. A. & Stilwell, M. (2001). Activity and spectrum of BMS 284756, a new des-F (6) quinolone, tested against strains of ciprofloxacin-resistant Gram-positive cocci. Diagnostic Microbiology and Infectious Disease 39, 1335.[ISI][Medline]
5 . Rhomberg, P. R., Biedenbach, D. J. & Jones, R. N. (2001). Activity of BMS 284756 (T-3811) tested against anaerobic bacteria, Campylobacter jejuni, Helicobacter pylori and Legionella spp. Diagnostic Microbiology and Infectious Disease 40, 459.[ISI][Medline]
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Fung-Tomc, J. C., Minassian, B., Kolek, B., Huczko, E., Aleksunes, L., Stickle, T. et al. (2000). Antibacterial spectrum of a novel des-fluoro (6) quinolone, BMS-284756. Antimicrobial Agents and Chemotherapy 44, 33516.
7 . Cevenini, R., Donati, M., Sambri, V., Rumpianesi, F. & La Placa, M. (1987). Reactivity of elementary bodies of Chlamydia trachomatis LGV2 with monoclonal antibodies specific for the major outer membrane protein. FEMS Microbiology Letters 42, 4751.[ISI]
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Malay, S., Roblin, P. M., Reznik, T., Kutlin, A. & Hammerschlag, M. R. (2002). In vitro activities of BMS-284756 against Chlamydia trachomatis and recent clinical isolates of Chlamydia pneumoniae. Antimicrobial Agents and Chemotherapy 46, 5178.
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Donati, M., Rumpianesi, F., Marchetti, F., Sambri, V. & Cevenini, R. (1998). Comparative in vitro activity of levofloxacin against Chlamydia spp. Journal of Antimicrobial Chemotherapy 42, 6701.
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Donati, M., Fermepin, M. R., Olmo, A., DApote, L. & Cevenini, R. (1999). Comparative in vitro activity of moxifloxacin, minocycline and azithromycin against Chlamydia spp. Journal of Antimicrobial Chemotherapy 43, 8257.