Department of Microbiology, Bristol-Myers Squibb Company, Wallingford, CT 06492, USA
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Abstract |
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Introduction |
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Quinolones differ in their mechanisms of bacterial killing. These mechanisms affect their abilities to kill depending on the metabolic state of the bacteria. A quinolone that can kill bacteria regardless of an organism's metabolic state might have advantages in the bacteriological eradication of the infecting pathogen. All quinolones can kill actively dividing bacteria, although some newer fluoroquinolones can kill non-dividing cells.3,4 The bactericidal activity of some quinolones depends on protein and RNA synthesis in the target bacterium.
In this study, we determined the killing mechanisms of gatifloxacin against strains of staphylococci, pneumococci and Escherichia coli. Ciprofloxacin, trovafloxacin and norfloxacin were included as comparators, since they represent quinolones with different bacterial killing mechanisms.24
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Materials and methods |
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Gatifloxacin was obtained from Kyorin Pharmaceutical Co., Ltd, Tochigi, Japan; ciprofloxacin was prepared at Bristol-Myers Squibb France, Montpellier, France; trovafloxacin was obtained from Pfizer Inc., New York, NY, USA; and norfloxacin, chloramphenicol and rifampicin were purchased from Sigma Chemical Co., St Louis, MO, USA.
Bacterial strains
Seven clinical isolates of E. coli (two strains), Streptococcus pneumoniae (three strains) and methicillin-resistant Staphylococcus aureus (MRSA; two strains), collected from diverse geographical areas, were tested. In an attempt to study strains that might represent the bacterial species, strains selected for testing had quinolone MICs close to the modal quinolone MICs for the respective species.
MIC determination
MICs were determined by the agar dilution method as recommended by the NCCLS,5 using bacterial inocula of c. 1.5 x 104 cfu/spot. The MIC was defined as the lowest concentration of antimicrobial agent that prevented visible growth.
OBC determination
The OBC was established by determining the viable counts at 0 and 6 h post-exposure of the bacteria to various concentrations of the quinolone. The OBC is the drug concentration yielding maximum reduction in viable cell counts.
Timekill analysis
Timekill analysis was performed in MuellerHinton broth (supplemented with 7% lysed horse blood for Streptococcus pneumoniae) using starting bacterial inocula of 105106 cfu/mL. Cells were grown to logarithmic phase with 1 h pre-incubation in fresh broth before the addition of quinolone. The initial killing rate was determined by removing aliquots of the culture at 0, 1, 2 and 3 h postinoculation with E. coli, and in addition, at 4 h for staphylococci, and at 4, 5 and 6 h for pneumococci. The duration of sampling for viability determination reflects the bacterial species-dependent rate of killing by quinolones.1 Cell counts determined on culture samples taken immediately before and after quinolone addition were similar, suggesting that any drug carryover had minimal effect on viable counts. All plates for colony counts were incubated at 35°C for up to 48 h before any were considered to have no growth. The quinolone concentrations used for mechanism of killing determination were at their OBCs, and for S. pneumoniae A28275, at 10 x MIC. To distinguish the different killing mechanisms, the requirement for bacterial protein or RNA synthesis was determined by the addition of chloramphenicol or rifampicin (at 0.5 x MIC), respectively. The necessity of dividing cells for killing was tested by performing timekill analysis for E. coli and MRSA in phosphate-buffered saline (PBS), or for pneumococci in PBS supplemented with 7% horse serum.6
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Results and discussion |
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Quinolone killing rates are organism group dependent. Quinolones kill enteric bacilli more rapidly than staphylococci, which in turn are killed more rapidly than streptococci and enterococci.1 Moreover, quinolones reportedly differ in their killing mechanisms depending on the organism group. For example, ciprofloxacin reportedly kills both dividing and non-dividing E. coli cells, but only dividing staphylococcal cells.9,10 In this study, however, ciprofloxacin was able to kill the two staphylococcal strains under non-growing conditions, but not the E. coli cells suspended in saline (Table 2). Differences in testing media and other experimental differences, such as use of exponentially growing cells versus overnight cultures for the bacterial inoculum, could account for differences in the killing profile. Nonetheless, quinolone killing may differ for strains within the same organism group, as with trovafloxacin killing of the two E. coli strains.
In summary, relative to the comparative quinolones assessed, gatifloxacin exhibited a more favourable bactericidal profile. Gatifloxacin killed staphylococci and E. coli regardless of the metabolic state of the microbe. It also kills pneumococci in the absence of protein and RNA synthesis.
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Notes |
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References |
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2 . Morrissey, I. (1997). Bactericidal index: a new way to assess quinolone bactericidal activity in vitro. Journal of Antimicrobial Chemotherapy 39, 7137. [Abstract]
3 . Smith, J. T. (1984). Awakening the slumbering potential of the 4-quinolone antibiotics. Pharmaceutical Journal 233, 299305.
4 . Lewin, C. S., Amyes, S. G. B. & Smith, J. T. (1989). Bactericidal activity of enoxacin and lomefloxacin against Escherichia coli K16. European Journal of Clinical Microbiology and Infectious Disease 8, 7313. [ISI][Medline]
5 . National Committee for Clinical Laboratory Standards. (1997). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow AerobicallyFourth Edition: Approved Standard M7-A4. NCCLS, Wayne, PA.
6 . Morrissey, I. (1996). Bactericidal activity of trovafloxacin (CP-99,219). Journal of Antimicrobial Chemotherapy 38, 10616. [Abstract]
7 . Turnidge, J. (1999). Pharmacokinetics and pharmacodynamics of fluoroquinolones. Drugs 58, Suppl. 2, 2936.
8 . Gradelski, E., Kolek, B., Bonner, D. P., Valera, L., Minassian, B. & Fung-Tomc, J. (2001). Activity of gatifloxacin and ciprofloxacin in combination with other antimicrobial agents. International Journal of Antimicrobial Agents 17, 1037. [ISI][Medline]
9 . Lewin, C. S. & Smith, J. T. (1988). Bactericidal mechanisms of ofloxacin. Journal of Antimicrobial Chemotherapy 22, Suppl. C, 18. [ISI][Medline]
10 . Morrissey, I. & Smith, J. T. (1995). Bactericidal activity of the new 4-quinolones DU-6859a and DV-7751a. Journal of Medical Microbiology 43, 48. [Abstract]
Received 12 March 2001; returned 20 July 2001; revised 9 September 2001; accepted 8 October 2001