Istituto di Igiene e Medicina Preventiva, Università degli Studi di Milano, via Pascal 38, 20133 Milano, Italy
Received 9 June 2005; returned 30 June 2005; revised 18 July 2005; accepted 7 August 2005
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Abstract |
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Methods: In vitro susceptibility to flucytosine, fluconazole, itraconazole, posaconazole, voriconazole and caspofungin was performed by broth microdilution following the NCCLS guidelines. Biofilm production was measured using the XTT reduction assay in 59 isolates selected as representative of different patterns of susceptibility to flucytosine and azoles.
Results: MICs (mg/L) at which 90% of the strains were inhibited were 0.25 for flucytosine, 0.25 for caspofungin, 4 for fluconazole and 0.06 for itraconazole, voriconazole and posaconazole. Flucytosine resistance was detected in five isolates and was associated with serotype B in 2/29 and serotype A in 3/346. Resistance to fluconazole was detected in 10 isolates; nine of these exhibited reduced susceptibility to the other azoles. Among the 10 patients with fluconazole-resistant C. albicans bloodstream infection, only one, an AIDS patient, had been previously treated with fluconazole. Biofilm production was observed in 23 isolates (39%) and was significantly associated with serotype B. No relationship was detected with the pattern of antifungal susceptibility.
Conclusions: Resistance is uncommon in C. albicans isolates recovered from blood cultures, while biofilm production is a relatively frequent event. Periodic surveillance is warranted to monitor the incidence of in vitro antifungal resistance as well as of biofilm production.
Keywords: flucytosine , caspofungin , azoles , voriconazole , posaconazole
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Introduction |
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The introduction of fluconazole in the 1990s has improved the outcome of Candida BSIs. However, the formation of biofilms on inert or biological surfaces frequently associated with deep seated candidosis enhances resistance to antimicrobial agents and protection from host defences making these infections refractory to conventional therapy.5 Amphotericin B lipid formulations and echinocandins have been shown to have activity against Candida biofilms as the inhibition of polysaccharide production could lead to lysis and dissolution of the extracellular matrix.5
The aim of the present report is to investigate the antifungal susceptibility pattern of 375 C. albicans isolates recovered from blood cultures during the ECMM survey of candidaemia (09/1997 to 12/1999) in Lombardia, Italy,6 and to test the ability to form biofilm.
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Materials and methods |
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A total of 375 C. albicans strains isolated from blood (the first isolate from each episode) during the ECMM survey of candidaemia were studied. Thirty-five medical centres in Lombardia, Italy, participated in the survey from September 1997 to December 1999.
The strains received by the coordinating centre were subcultured on Chromagar Candida medium (CHROMagar Microbiology, Paris, France) to ensure viability and purity.
Yeast identification was checked by production of germ tubes in serum and chlamydospores in potatocarrotox gall agar. Serotype was identified by slide agglutination test with specific antisera (Candida Check; Iatron Laboratories, Tokyo, Japan).
Isolates were stored as suspensions in distilled water at room temperature until needed.
Susceptibility testing
The antifungals tested were fluconazole (Pfizer Central Research, Sandwich, UK), itraconazole (Janssen Research Foundation, Beerse, Belgium), voriconazole (Pfizer), posaconazole (Schering-Plough Research Institute, Kenilworth, NJ, USA), flucytosine (Sigma-Chemical, Milano, Italy) and caspofungin (Merck & Co., Whitehouse Station, NJ, USA). Susceptibility was determined by broth microdilution method performed following the recommendations of the NCCLS.7 Testing was performed in RPMI 1640 without sodium bicarbonate (Sigma-Chemical) and buffered to pH 7.0 with 0.165 M MOPS (Sigma-Chemical) and supplemented with 2% glucose and 0.03% L-glutamine (Sigma-Chemical). For strains exhibiting a significant trailing effect when tested against azoles, susceptibility was also performed with Casitone broth or RPMI broth with Alamar Blue (Trek Diagnostic Systems, Inc., West Sussex, UK). Candida parapsilosis ATCC 22019 was used as a quality control strain. The interpretative breakpoints suggested in the NCCLS document were adopted.
Biofilm production
Biofilm production was investigated as described by Ramage et al.8 in 59 isolates. The isolates were selected as being representative of different serotypes and of different patterns of susceptibility to flucytosine and azoles (resistant, susceptible and those showing a trailing effect). Briefly, isolates were suspended in RPMI 1640 broth supplemented with L-glutamine and buffered with MOPS, and 100 µL inoculated in flat-bottom 96-well microtitre plates. Biofilm production was measured after 24 h by using a 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide (XTT; Sigma-Chemical) reduction assay. XTT assay absorbance was read spectrophotometrically (Multiskan MS; Labsystems, Needham Heights, MA, USA) at 490 nm. The percentage transmittance (%T), calculated from absorbance, was inversely proportional to the cellular density of the biofilm. Biofilm production was scored as 6+ (%T 5), 5+ (%T 610), 4+ (%T 1120), 3+ (%T 2140), 2+ (%T 4160) or 1+ (%T > 60). Isolates showing scores of 6+ or 5+ were considered good producers of biofilm.
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Results and discussion |
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Resistance to fluconazole (MIC 64 mg/L) was detected in 10 isolates (2.7%), all identified as serotype A. This is in agreement with the negligible proportion of resistance among C. albicans bloodstream isolates reported elsewhere.1,911 Among the 10 patients with fluconazole-resistant C. albicans BSI only one, an AIDS patient, had been treated with fluconazole during the 2 weeks preceding candidaemia. The isolate from this patient also exhibited resistance to flucytosine.
Isolates were highly susceptible to the other azoles. MIC90s of itraconazole, voriconazole and posaconazole were 0.06 mg/L. However, as previously noted,1,10 the fluconazole-resistant isolates tended to be less susceptible to the other azoles and nine of these exhibited reduced susceptibility to itraconazole, voriconazole and posaconazole.
Caspofungin MICs ranged from 0.03 to 1 mg/L, and 86% and 98% of isolates were inhibited by 0.12 and 0.25 mg/L of caspofungin, respectively. Eighteen isolates showed the well described12 paradoxical effect of a regrowth at highest concentrations (4 and 8 mg/L) after growth inhibition in the wells with lower echinocandin levels (0.250.5 to 2). This phenomenon was more common reading after 48 h of incubation. The paradoxical effect was more frequent in serotype B than in serotype A strains (9 out of 29 versus 9 out of 346, P 0.001).
Biofilm production was observed in 23 isolates (39%) out of the 59 tested strains and was significantly associated with serotype B (19/28 versus 4/31 in serotype A, P 0.001). No relationship between biofilm production (scores of 6+ and 5+) and a specific pattern of susceptibility to fluconazole was detected (Figure 1). This lack of correlation could be explained by the use of planktonic populations in the antifungal susceptibility testing performed according to NCCLS guidelines.7
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Therefore, periodic surveillance is warranted to monitor the incidence of in vitro antifungal resistance as well as of biofilm production.
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Acknowledgements |
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References |
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