1 S. Microbiology and 2 S. Pharmacy, Hospital General Universitario de Elche, Elche, 03203 Spain
Received 8 October 2003; returned 25 November 2003; revised 9 December 2003; accepted 18 December 2003
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Abstract |
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Methods: We determined the antibiotic concentration that prevents the selection of resistant mutants following inoculation with a high mycobacteria inoculum on Middlebrook 7H11 plates with serial dilutions of the antibiotics in 224 M. tuberculosis isolates.
Results: Fifty percent of the strains exhibited values of MPC (MPC50) lower than 0.8, 0.6, 0.4, 0.4 and 0.6 mg/L for ciprofloxacin, levofloxacin, gatifloxacin, moxifloxacin and linezolid, respectively. If 90% of the strains are considered (MPC90), the values are seen to rise to 2, 1.8, 1, 1.2 and 1.2 mg/L, respectively.
Conclusions: When we compare this parameter with the drug levels in serum and tissue, it can be seen that ciprofloxacin is the least useful of the fluoroquinolones studied, whereas moxifloxacin appears to be the most active. Linezolid exhibits excellent activity against this microorganism (MPC90 1.2 mg/L and AUC 140.3 mg·h/L) and this makes us consider that its usefulness in the treatment of this pathology should be thoroughly evaluated.
Keywords: tuberculosis, drug activity, fluoroquinolones, oxazolidinones
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Introduction |
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Materials and methods |
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Two hundred and twenty-four M. tuberculosis clinical isolates were obtained in the Elche health district between 1992 and 2003. For each strain, the following data were known: date of isolation, site of the disease, HIV serology of the patient and susceptibility to the antibiotics studied.3,4 The strains were kept frozen at 70°C. The strain Mt 14323 was processed for quality control.
Mutant prevention concentration (MPC)
The inocula were prepared from a culture in the Lowenstein medium; 5 µL aliquots of a 2 McFarland suspension were inoculated on Middlebrook 7H11 plates (approximately 1010 microorganisms) with dilutions of antibiotics (mg/L) from 0.1 up to 4. The plates were incubated at 37°C for 4 weeks. The MPCs were estimated by determining the concentration that allowed recovery of no colony.2
Antibiotics
The following antibiotics were used: ciprofloxacin (Bayer), levofloxacin (Hoechst), gatifloxacin (Bristol-Myers Squibb), moxifloxacin (Bayer) and linezolid (Pharmacia).
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Results |
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Conclusions |
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Failure, relapses and the selection of resistant mutants during treatment are complex phenomena associated with the characteristics of the microorganism together with the characteristics and dosage of the drug used. Also, the drug levels reached vary depending on the type of tissue and patient concerned.8
Ciprofloxacin is the fluoroquinolone that has been most widely used in the treatment of tuberculosis. It has been used as a second line drug because it exhibits less activity than traditional treatments.9 However, the usefulness of the drug in the treatment of tuberculosis10 and its bactericidal activity11 have been seen to be dose-dependent, since the area under the curve varies between 4.8 and 30.8 mg·h/L depending on the dose given. Ciprofloxacin has been reported12 to reach a maximum concentration in serum of 6.21 mg/L and a concentration between 1.7 and 7.1 times higher in pulmonary tissue. However, the serum and tissue levels decrease 812 h after the drug has been administered (0.13 mg/L and 0.67 mg/kg, respectively);13 therefore, it is important to take into consideration the period of time that the MPC is greater than the drug concentration in tissue.14
When we compare the activity of the different fluoroquinolones (Table 2), ciprofloxacin is the least active. This is shown by the low values of the quotient AUCtot/MPC and the fact that its alveolar macrophage levels are lower than those of other fluoroquinolones such as levofloxacin (13.8 versus 6.8 mg/L).15
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Twelve hours after the drugs were administered, the following concentrations of gatifloxacin and moxifloxacin (which were higher than the MPC for most of the strains) were achieved: in epithelial lining fluid (2.98 and 5.9 mg/L), alveolar macrophages (61.95 and 54.1 mg/L) and bronchial mucosa (3.00 and 2.0 mg/kg), respectively.20,21 However, low doses may be found in certain types of tissue: in CSF 1 h after administration, the gatifloxacin concentration is between 0.46 and 1.84 mg/L depending on the dose given22 and 2 h after administration, the levofloxacin concentration is 1.99 mg/L.23 On the other hand, moxifloxacin reaches higher concentrations in CSF: in a rabbit model, between 9.5 and 1.8 mg/L is found depending on the dose given24 and in bone, the concentration reaches 6.3 mg/L.25
Our data confirm the good activity of moxifloxacin and gatifloxacin in murine models, where they are seen to exhibit similar bactericidal activity to that of isoniazid, although their activity is dependent on the dose administered.26,27
The data obtained on linezolid indicate excellent activity against the M. tuberculosis strains studied, as a result of the high drug concentration in serum and tissue (Table 2), with a concentration of 10.7 mg/kg being reached in bronchial mucosa, 8.1 mg/L in alveolar macrophages and 25.1 mg/L in epithelial lining fluid.28,29 These data confirm the results obtained with a murine model.30
The key to carrying out effective treatment and preventing the selection of resistant mutants is to choose the right antibiotic, the correct dose and complete the treatment, or to use two or more drugs.31,32
In this way, the drug concentration in serum and tissue will be above the MPC for the M. tuberculosis strain for the longest period possible. Therefore, it can be of help in determining the true usefulness of each individual drug in the treatment of different clinical situations, especially in cases of multidrug-resistant microorganisms or patients who find it difficult to adhere to treatment.33 Our findings indicate that levofloxacin, gatifloxacin and especially moxifloxacin are good alternative therapies and superior to ciprofloxacin. Also, linezolid appears to be a drug that achieves very good results and so its true usefulness in the treatment of tuberculosis should be evaluated.
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Footnotes |
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References |
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2
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