The European Confederation of Medical Mycology (ECMM) survey of candidaemia in Italy: in vitro susceptibility of 375 Candida albicans isolates and biofilm production

Anna Maria Tortorano*, Anna Prigitano, Emanuela Biraghi, Maria Anna Viviani on behalf of the FIMUA–ECMM Candidaemia Study Group

Istituto di Igiene e Medicina Preventiva, Università degli Studi di Milano, via Pascal 38, 20133 Milano, Italy


* Corresponding author. Tel: +39-02-50315144/145; Fax: +39-02-50315098; E-mail: annamaria.tortorano{at}unimi.it

Received 9 June 2005; returned 30 June 2005; revised 18 July 2005; accepted 7 August 2005


    Abstract
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 Abstract
 Introduction
 Materials and methods
 Results and discussion
 References
 
Objectives: To investigate the in vitro antifungal susceptibility pattern of 375 Candida albicans bloodstream isolates recovered during the European Confederation of Medical Mycology survey of candidaemia performed in Lombardia, Italy and to test the ability to form biofilm.

Methods: In vitro susceptibility to flucytosine, fluconazole, itraconazole, posaconazole, voriconazole and caspofungin was performed by broth microdilution following the NCCLS guidelines. Biofilm production was measured using the XTT reduction assay in 59 isolates selected as representative of different patterns of susceptibility to flucytosine and azoles.

Results: MICs (mg/L) at which 90% of the strains were inhibited were ≤0.25 for flucytosine, 0.25 for caspofungin, 4 for fluconazole and 0.06 for itraconazole, voriconazole and posaconazole. Flucytosine resistance was detected in five isolates and was associated with serotype B in 2/29 and serotype A in 3/346. Resistance to fluconazole was detected in 10 isolates; nine of these exhibited reduced susceptibility to the other azoles. Among the 10 patients with fluconazole-resistant C. albicans bloodstream infection, only one, an AIDS patient, had been previously treated with fluconazole. Biofilm production was observed in 23 isolates (39%) and was significantly associated with serotype B. No relationship was detected with the pattern of antifungal susceptibility.

Conclusions: Resistance is uncommon in C. albicans isolates recovered from blood cultures, while biofilm production is a relatively frequent event. Periodic surveillance is warranted to monitor the incidence of in vitro antifungal resistance as well as of biofilm production.

Keywords: flucytosine , caspofungin , azoles , voriconazole , posaconazole


    Introduction
 Top
 Abstract
 Introduction
 Materials and methods
 Results and discussion
 References
 
Although the emergence of non-albicans Candida spp. as a cause of bloodstream infection (BSI) has been reported in all surveillance programmes,1 Candida albicans remains the predominant species recovered. In the large prospective survey performed by the European Confederation of Medical Mycology (ECMM) C. albicans was identified in 1178 out of 2089 reported episodes (56%),2 ranging from 43% in Spain3 to 67% in Sweden4 and was responsible for more than half of the cases in all the patient populations except in patients with haematological malignancies.2

The introduction of fluconazole in the 1990s has improved the outcome of Candida BSIs. However, the formation of biofilms on inert or biological surfaces frequently associated with deep seated candidosis enhances resistance to antimicrobial agents and protection from host defences making these infections refractory to conventional therapy.5 Amphotericin B lipid formulations and echinocandins have been shown to have activity against Candida biofilms as the inhibition of polysaccharide production could lead to lysis and dissolution of the extracellular matrix.5

The aim of the present report is to investigate the antifungal susceptibility pattern of 375 C. albicans isolates recovered from blood cultures during the ECMM survey of candidaemia (09/1997 to 12/1999) in Lombardia, Italy,6 and to test the ability to form biofilm.


    Materials and methods
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 Abstract
 Introduction
 Materials and methods
 Results and discussion
 References
 
Isolates

A total of 375 C. albicans strains isolated from blood (the first isolate from each episode) during the ECMM survey of candidaemia were studied. Thirty-five medical centres in Lombardia, Italy, participated in the survey from September 1997 to December 1999.

The strains received by the coordinating centre were subcultured on Chromagar Candida medium (CHROMagar Microbiology, Paris, France) to ensure viability and purity.

Yeast identification was checked by production of germ tubes in serum and chlamydospores in potato–carrot–ox gall agar. Serotype was identified by slide agglutination test with specific antisera (Candida Check; Iatron Laboratories, Tokyo, Japan).

Isolates were stored as suspensions in distilled water at room temperature until needed.

Susceptibility testing

The antifungals tested were fluconazole (Pfizer Central Research, Sandwich, UK), itraconazole (Janssen Research Foundation, Beerse, Belgium), voriconazole (Pfizer), posaconazole (Schering-Plough Research Institute, Kenilworth, NJ, USA), flucytosine (Sigma-Chemical, Milano, Italy) and caspofungin (Merck & Co., Whitehouse Station, NJ, USA). Susceptibility was determined by broth microdilution method performed following the recommendations of the NCCLS.7 Testing was performed in RPMI 1640 without sodium bicarbonate (Sigma-Chemical) and buffered to pH 7.0 with 0.165 M MOPS (Sigma-Chemical) and supplemented with 2% glucose and 0.03% L-glutamine (Sigma-Chemical). For strains exhibiting a significant trailing effect when tested against azoles, susceptibility was also performed with Casitone broth or RPMI broth with Alamar Blue (Trek Diagnostic Systems, Inc., West Sussex, UK). Candida parapsilosis ATCC 22019 was used as a quality control strain. The interpretative breakpoints suggested in the NCCLS document were adopted.

Biofilm production

Biofilm production was investigated as described by Ramage et al.8 in 59 isolates. The isolates were selected as being representative of different serotypes and of different patterns of susceptibility to flucytosine and azoles (resistant, susceptible and those showing a trailing effect). Briefly, isolates were suspended in RPMI 1640 broth supplemented with L-glutamine and buffered with MOPS, and 100 µL inoculated in flat-bottom 96-well microtitre plates. Biofilm production was measured after 24 h by using a 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide (XTT; Sigma-Chemical) reduction assay. XTT assay absorbance was read spectrophotometrically (Multiskan MS; Labsystems, Needham Heights, MA, USA) at 490 nm. The percentage transmittance (%T), calculated from absorbance, was inversely proportional to the cellular density of the biofilm. Biofilm production was scored as 6+ (%T ≤ 5), 5+ (%T 6–10), 4+ (%T 11–20), 3+ (%T 21–40), 2+ (%T 41–60) or 1+ (%T > 60). Isolates showing scores of 6+ or 5+ were considered good producers of biofilm.


    Results and discussion
 Top
 Abstract
 Introduction
 Materials and methods
 Results and discussion
 References
 
Table 1 summarizes the in vitro susceptibilities of the 375 bloodstream C. albicans isolates to flucytosine, fluconazole, itraconazole, voriconazole, posaconazole and caspofungin. MICs (mg/L) at which 50% (MIC50) and 90% (MIC90) of the strains were inhibited and the range of MICs are reported. Ninety-nine isolates (26.4%) exhibited a significant trailing effect when tested against azoles. These isolates, however, tested susceptible in repeated tests performed using Casitone broth or RPMI broth with/without Alamar Blue. MICs for the quality control strain, C. parapsilosis ATCC 22019, were within the expected range.


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Table 1. MIC50, MIC90 and range of MICs (mg/L) for 375 C. albicans bloodstream isolates and MIC for the quality control strain

 
Only five isolates (1.3%) showed resistance to flucytosine (MIC ≥ 32 mg/L) confirming the low rate of resistance reported in the literature.9 Resistance was associated with serotype B in 2/29 (6.9%) and with serotype A in 3/346 (0.9%).

Resistance to fluconazole (MIC ≥ 64 mg/L) was detected in 10 isolates (2.7%), all identified as serotype A. This is in agreement with the negligible proportion of resistance among C. albicans bloodstream isolates reported elsewhere.1,911 Among the 10 patients with fluconazole-resistant C. albicans BSI only one, an AIDS patient, had been treated with fluconazole during the 2 weeks preceding candidaemia. The isolate from this patient also exhibited resistance to flucytosine.

Isolates were highly susceptible to the other azoles. MIC90s of itraconazole, voriconazole and posaconazole were 0.06 mg/L. However, as previously noted,1,10 the fluconazole-resistant isolates tended to be less susceptible to the other azoles and nine of these exhibited reduced susceptibility to itraconazole, voriconazole and posaconazole.

Caspofungin MICs ranged from 0.03 to 1 mg/L, and 86% and 98% of isolates were inhibited by 0.12 and 0.25 mg/L of caspofungin, respectively. Eighteen isolates showed the well described12 paradoxical effect of a regrowth at highest concentrations (4 and 8 mg/L) after growth inhibition in the wells with lower echinocandin levels (0.25–0.5 to 2). This phenomenon was more common reading after 48 h of incubation. The paradoxical effect was more frequent in serotype B than in serotype A strains (9 out of 29 versus 9 out of 346, P ≤ 0.001).

Biofilm production was observed in 23 isolates (39%) out of the 59 tested strains and was significantly associated with serotype B (19/28 versus 4/31 in serotype A, P ≤ 0.001). No relationship between biofilm production (scores of 6+ and 5+) and a specific pattern of susceptibility to fluconazole was detected (Figure 1). This lack of correlation could be explained by the use of planktonic populations in the antifungal susceptibility testing performed according to NCCLS guidelines.7



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Figure 1. Biofilm production according to different patterns of fluconazole susceptibility.

 
In conclusion, the low proportion of antifungal resistance detected in this survey, consistent with data reported in both European and American surveillance programmes,1,911 appears reassuring and supports the indication to perform antifungal susceptibility testing only in case of failure of response to initial therapy or of breakthrough candidaemia in patients receiving fluconazole prophylaxis. However, the relatively high rate of biofilm production among the tested isolates is noteworthy, as biofilm lifestyle has been demonstrated to increase levels of resistance to the most commonly used antifungal agents.

Therefore, periodic surveillance is warranted to monitor the incidence of in vitro antifungal resistance as well as of biofilm production.


    Acknowledgements
 
We thank Janssen Research Foundation, Merck Sharp & Dohme, Pfizer Central Research and Schering-Plough Research Institute, respectively, for providing itraconazole, caspofungin, voriconazole and posaconazole. The results of this study were presented in part at the Tenth Congress of the European Confederation of Medical Mycology, Wroclaw, Poland, 2004. The study was in part supported by a grant from Ministero dell' Università e della Ricerca Scientifica e Tecnologica (COFIN 2001-065221). FIMUA–ECMM Candidaemia Study Group participants were: A. Astolfi, C. Ossi, S. Perin, C. Bonaccorso, L. Garlaschi, G. Viola, M. Saudelli, S. Frugoni, A. Mauri, R. Passerini (Milano); C. Farina (Bergamo); M. Tejada (S. Donato M); C. Cavanna (Pavia); A. Raballo (Mantova); A. Grossi (Treviglio); C. Bezzi (Magenta); M. Spinelli (Como); S. Bramati (Monza); G. Pinsi (Brescia); L. Colombo (Desio); L. Ferrari (Cremona); P. Troupioti (Sondalo); G.L. Lombardi (Varese); M. Arghittu (Melegnano); C. Agrappi (Legnano); L. Sturla (Gallarate); G. Gialluca (Rho); M.G. Musmanno (Saronno); A. Ceraminiello (Lodi); P. Casella (Vimercate); P. Porri (Voghera); S. Pierdomenico (Busto Arsizio); S. Gualterotti (Cinisello Balsamo); and R. Sala (Merate).


    References
 Top
 Abstract
 Introduction
 Materials and methods
 Results and discussion
 References
 
1. Pfaller MA, Deikema DJ. Role of sentinel surveillance of candidemia; trends in species distribution and antifungal susceptibility. J Clin Microbiol 2002; 40: 3551–7.[Free Full Text]

2. Tortorano AM, Peman J, Bernhardt H et al. Epidemiology of candidaemia in Europe: results of 28-month European Confederation of Medical Mycology (ECMM) hospital-based surveillance study. Eur J Clin Microbiol Infect Dis 2004; 23: 317–22.[CrossRef][ISI][Medline]

3. Peman J, Canton E, Gobernado M et al. Epidemiology and antifungal susceptibility of Candida species isolated from blood: results of a 2-year multicentre study in Spain. Eur J Clin Microbiol Infect Dis 2005; 24: 23–30.[CrossRef][ISI][Medline]

4. Klingspor L, Tornqvist E, Johansson A et al. A prospective epidemiological survey of candidaemia in Sweden. Scand J Infect Dis 2004; 36: 52–5.[CrossRef][ISI][Medline]

5. Ramage G, Saville SP, Thomas DP et al. Candida biofilm: an update. Eukaryot Cell 2005; 4: 633–8.[Free Full Text]

6. Tortorano AM, Biraghi E, Astolfi A et al. European Confederation of Medical Mycology (ECMM) prospective survey of candidaemia: report from one Italian region. J Hosp Infect 2002; 51: 297–304.[CrossRef][ISI][Medline]

7. National Committee for Clinical Laboratory Standards. Reference Method for Broth Dilution Testing of Yeasts—Second Edition: Approved Standard M27-A2. NCCLS, Wayne, PA, USA, 2002.

8. Ramage G, vande Walle K, Wickes BL et al. Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms. Antimicrob Agents Chemother 2001; 45: 2475–9.[Abstract/Free Full Text]

9. Sanglard D, Odds FC. Resistance of Candida species to antifungal agents: molecular mechanisms and clinical consequences. Lancet Infect Dis 2002; 2: 73–85.[CrossRef][ISI][Medline]

10. Pfaller MA, Messer SA, Boyken L et al. In vitro activities of voriconazole, posaconazole and fluconazole against 4,169 clinical isolates of Candida spp. and Cryptococcus neoformans collected during 2001 and 2002 in the ARTEMIS global antifungal surveillance program. Diagn Microbiol Infect Dis 2004; 48: 201–5.[CrossRef][ISI][Medline]

11. Hajjeh RA, Sofair AN, Harrison LH et al. Incidence of bloodstream infections due to Candida species and in vitro susceptibilities of isolates collected from 1998 to 2000 in a population-based active surveillance program. J Clin Microbiol 2004; 42: 1519–27.[Abstract/Free Full Text]

12. Stevens DA, Espiritu M, Parmar R. Paradoxical effect of caspofungin: reduced activity against Candida albicans at high drug concentrations. Antimicrob Agents Chemother 2004; 48: 3407–11.[Abstract/Free Full Text]





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