1 Department of Medical Microbiology (G4), Faculty of Health Sciences, University of the Free State, Bloemfontein; 2 Medical Research Council, Cape Town, South Africa
Received 20 February 2004; returned 19 March 2004; revised 7 April 2004; accepted 10 April 2004
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Abstract |
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Methods: Metronidazole susceptibility was determined in 99 strains of anaerobic Gram-positive cocci and PCR amplification for the nim gene carried out on 61 strains (metronidazole MIC 0.5 mg/L).
Results: The nimB gene was detected in 34% (21/61) of the strains. These included two highly resistant Finegoldia magna strains (MICs >128 mg/L). The nimB gene was, however, also demonstrated in 90% (19/21) of susceptible strains.
Conclusions: Although the nimB gene may be implicated in the high-level metronidazole resistance in 2 F. magna strains, the alarmingly high prevalence of the nimB gene in anaerobic Gram-positive cocci cannot be directly associated with resistance and the possibility of a silent nimB gene should be considered.
Keywords: anaerobic Gram-positive cocci , metronidazole resistance , nim genes
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Introduction |
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Materials and methods |
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Ninety-nine anaerobic Gram-positive cocci were isolated during 19961997 (as Peptostreptococcus spp.) from clinically significant infections in patients in the Universitas and Pelonomi Hospitals in Bloemfontein.6
MIC determination
MICs of metronidazole (supplied by Sigma, St Louis, MO, USA) were determined by the National Committee for Clinical Laboratory Standards (NCCLS) agar dilution methods.8 The susceptibility breakpoint for metronidazole (16 mg/L) was used as suggested by the NCCLS.8 Wilkins Chalgren agar (Mast Diagnostics, Merseyside, UK) was supplemented with 5% lysed horse blood to enhance growth of these fastidious bacteria. Sixty-one isolates comprising 35 Peptostreptococcus anaerobius, nine Finegoldia magna, eight Anaerococcus prevotii, four Peptoniphilus asaccharolyticus, two Peptoniphilus indolicus and three Micromonas micros strains with metronidazole MICs
0.5 mg/L were selected for PCR amplification.
Nim gene PCR and sequencing
Cell lysates were obtained from overnight cultures, as described by Lubbe et al.2 Lysis was enhanced by the addition of 10 mg/L mutanolysin to the cell suspension. PCR assays were carried out using the primers Nim-3 and Nim-5 as described by Trinh & Reysset.5 The primers were designed for the universal amplification of all known nim genes to produce a PCR product of 458 bp.5 Amplification conditions were as previously described by Lubbe et al.6 using an annealing temperature of 52°C. PCR products from Bacteroides vulgatus (BV-17) and B. fragilis (BF-8) control strains were used as references.5
Fragments of approximately 458 bp in any of the isolates were regarded as presumptive positives and excised from a 1% agarose gel, using a GFX PCR DNA and Gel Band Purification kit (Amersham Pharmacia Biotech, NJ, USA). Automated sequencing of PCR products was carried out using a BigDye Terminator sequencing kit (Applied Biosystems, CA, USA). Reaction mixtures for sequencing were analysed on a Sequence Navigator gel and computer analysis was carried out with ABI PRISM, Model 377 (Perkin-Elmer). Sequences were electronically submitted for identification into the GenBank nucleotide database via the website of the National Center for Biotechnology Information (NCBI).
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Results and discussion |
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A very important aspect of this study is that the gene was found in two highly resistant F. magna strains. F. magna is probably the most pathogenic of Gram-positive anaerobic cocci found in human specimens and has been recovered in pure culture from a range of serious infections, some of which have been fatal.9 Should the nimB gene be confirmed to be implicated in the development of such high-level resistance, the problem could be more far reaching than was previously thought. Proportionately, the nimB genes were also more prevalent in the F. magna strains (56%) than in the other strains investigated (Table 1).
The role of the nimB gene in metronidazole resistance development in these anaerobic Gram-positive cocci is not immediately evident as the nimB gene was surprisingly found in 33% (19/58) of the metronidazole-susceptible strains, yet not in the one P. anaerobius strain with reduced susceptibility to metronidazole (MIC 8 mg/L). According to Haggoud et al., the positioning of nim genes relative to insertion sequence elements, with either strong or weak promoters, is crucial when assessing the input nim genes may have on phenotypic levels of metronidazole resistance, as every nim gene has been shown to be associated with an IS element.10 However, both nimA and nimB are directly preceded by the insertion sequence IS116810 and the electronic gene identification results also indicated the nimB gene being associated and preceded by the insertion sequence IS1168, in the resistant as well as the susceptible Gram-positive cocci.
In a recent study in our laboratory, nim genes were demonstrated in a wide range of clinically important anaerobic bacteria, such as B. fragilis group (nimA and nimB), P. bivia (nimA), C. bifermentans (nimA), A. odontolyticus (nimA) and Propionibacterium spp. (nimA)2 although worldwide they have only been described in Bacteroides spp. This is the first report of the nim gene being found in any of the anaerobic Gram-positive cocci, that have all previously been classified under the genus Peptostreptococcus, and they can now be added to the South African list. Of interest is the fact that they appear to be a very important source of the nimB gene in particular. The detection of nim genes in numerous anaerobic bacterial species calls for periodic metronidazole susceptibility testing in conjunction with monitoring for the prevalence of different nim gene types.
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Footnotes |
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References |
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2 . Lubbe, M. M., Stanley, K. & Chalkley, L. J. (1999). Prevalence of nim genes in anaerobic/facultative anaerobic bacteria isolated in South Africa. FEMS Microbiology Letters 172, 7983.[CrossRef][ISI][Medline]
3 . Carlier, J.-P., Sellier, N., Rager, M.-N. et al. (1997). Metabolism of a 5-nitroimidazole in susceptible and resistant isogenic strains of Bacteroides fragilis. Antimicrobial Agents and Chemotherapy 41, 14959.[Abstract]
4 . Pankuch, G. A., Jacobs, M. R. & Appelbaum, P. C. (1993). Susceptibilities of 428 Gram-positive and -negative anaerobic bacteria to Bay y3118 compared with their susceptibilities to ciprofloxacin, clindamycin, metronidazole, piperacillin, piperacillin-tazobactam, and cefoxitin. Antimicrobial Agents and Chemotherapy 37, 164954.[Abstract]
5 . Trinh, S. & Reysset, G. (1996). Detection by PCR of the nim genes encoding 5-nitroimidazole resistance in Bacteroides spp. Journal of Clinical Microbiology 34, 207884.[Abstract]
6 . Lubbe, M. M., Botha, P. L. & Chalkley, L. J. (1999). Comparative activity of eighteen antimicrobial agents against anaerobic bacteria isolated in South Africa. European Journal of Clinical Microbiology and Infectious Diseases 18, 4654.[CrossRef][ISI][Medline]
7 . Koch, C. L., Derby, P. & Abratt, V. R. (1998). In-vitro antibiotic susceptibility and molecular analysis of anaerobic bacteria isolated in Cape Town, South Africa. Journal of Antimicrobial Chemotherapy 42, 2458.[Abstract]
8 . National Committee for Clinical Laboratory Standards (1997). Methods for Antimicrobial Testing of Anaerobic Bacteria: Approved Standard M11-A3. NCCLS, Villanova, PA, USA.
9 . Krepel, C. J., Gohr, C. M., Walker, A. P. et al. (1992). Enzymatically active Peptostreptococcus magnus: association with site of infection. Journal of Clinical Microbiology 30, 23304.[Abstract]
10 . Haggoud, A., Ait M'Hand, J., Reysset, G. et al. (2001). Prevalence and characteristics of nim genes encoding 5-nitroimidazole resistance among Bacteroides strains isolated in Morocco. Microbial Drug Resistance 7, 17781.[CrossRef][ISI][Medline]