a Service des Maladies Infectieuses et Tropicales, b Laboratoire d'Immunologie INSERM U343, Hôpital de l'Archet and c Laboratoire de Virologie, Hôpital Pasteur, Nice, France
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Abstract |
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Introduction |
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T cell apoptosis appears to be upregulated using agonistic monoclonal antibodies to CD4 or Fas surface molecules.3,5 The gp120 HIV protein can stimulate the CD4 pathway and promote apoptosis of HIV-negative CD4+ T cells, and soluble gp120 has been detected in patients' blood.3,4 Recently, we have demonstrated that spontaneous T cell apoptosis was reduced by HAART, compared with combination treatments with two nucleoside analogues.6 We did not observe any correlation between the level of spontaneous T cell apoptosis and HIV plasma viraemia, suggesting that viral factors, and not the amount of virus present, could be responsible for T cell apoptosis. In this study, we have examined the relationship between the HIV viral load measurement and CD4-induced apoptosis as this pathway is thought to mimic the in vivo stimulation of CD4+ T cells via the gp120 molecule.
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Patients and methods |
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Patients were recruited from a large cohort of healthy donors and HIV-infected individuals, in whom spontaneous apoptosis and stimulation-induced apoptosis via surface molecules were studied.6,7 Only HIV-infected patients on HAART with two nucleoside analogues and a protease inhibitor were included in order to avoid the impact of different treatments on T cell apoptosis. Epidemiological and biological data were obtained with a computerized medical record.6 Determinations of peripheral T cell populations and T cell apoptosis by cytofluorometric techniques, and the HIV viral load measurement were performed on the same blood sample. HIV-1 RNA plasma levels were measured using commercially available assays, according to the instructions of the manufacturers. An undetectable viral load was defined as being less than the lowest quantification limit for each type of assay used at that time.
Apoptosis assay
Peripheral blood mononuclear cells (PBMC) were isolated from heparinized blood by centrifugation on a Ficoll Hypaque gradient, washed and resuspended in RPMI 1640 complemented with 20 mM HEPES, 2 mM l-glutamine, 10% heat-inactivated fetal calf serum, penicillin and streptomycin (Life Technologies, Cergy Pontoise, France). Cell viability was evaluated by Trypan Blue exclusion.
The mouse mAbs specific for human surface antigens used included: CD4 (clone O516), CD8 (clone L533) and CD3 (clone X3), produced in our laboratory. mAb coating used 200 µL of O516 (10 mg/L final concentration) in 48-well plates incubated for 1 h at 37°C. For the apoptosis assay, PBMC (5 x 105 cells/well) were cultured overnight in the medium or on coated plates.
Apoptotic cells were quantified using Hoechst 33342 dye (H342) which detects apoptosis-associated DNA degradation (Sigma Laboratory, l'Isle d'Abeau, France). Cultured cells were first incubated for 30 min at 4°C with CD3 and CD8 mAb (10 µg/mL) coupled with fluorescein isothiocyanate and phycoerythrin, respectively. Cells were thereafter washed in RPMI-FCS 10% and stained by incubation for 30 min at 37°C with H342 (1 mg/L final concentration). For each sample, 104 double-stained lymphocytes were analysed with Lysis II software in a FACStar flow cytometer (Becton Dickinson, Mountain View, CA, USA).
Spontaneous and CD4-induced T cell apoptosis were determined in the same experiment for all patients. Each was tested in duplicate. To assess the impact of viral load on T cell activation-induced apoptosis, we compared patients with a detectable viral load with patients with an undetectable one. We used Student's t-test or the Pearson correlation test as appropriate. A P value <0.05 was considered significant.
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Results |
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Discussion |
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Our results are in accordance with a previous study showing an association between the HIV viral load and activation-induced apoptosis of T cells in HIV-infected patients.8 However, in this latter work, antiretroviral treatments and their durations were not studied. Because antiretroviral treatments modulate apoptosis, we studied patients receiving two nucleosides and a protease inhibitor for a similar duration.
These results highlight the role of active viral replication on CD4+ T cell death. Even a low level of viral replication is able to upregulate activation-induced apoptosis of CD4+ T cells. Experimental studies have shown that HIV replication and/or HIV proteins such as gp 120 or Tat are needed to induce apoptosis.4,5 In addition, it has been shown that HIV-infected antigen-presenting cells (APC) were able to induce apoptosis of uninfected T cells.3,9 Thus, because infected APC are persistently infected over the natural course of the disease, we suggest that local bursts of viral replication, as described in lymphoid tissues,10 induce deleterious interactions with uninfected CD4+ T cells, leading to activation-induced apoptosis. Further studies are needed to confirm this hypothesis.
CD4-induced apoptosis has been reported to be dependent on APC, leading to an upregulation of CD8+ T cell death.3 However, we found no relationship between spontaneous or CD4-induced apoptosis of CD8+ T cell and viral load.
Taken together, our results and others have important implications for the understanding of the partial immune reconstitution in HIV-infected patients on HAART. The precise understanding of cellular interactions occurring in HIV infection could lead to new therapeutic interventions.
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Acknowledgments |
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Notes |
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References |
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8 . Karmochkine, M., Parizot, C., Calvez, V., Coutellier, A., Herson, S., Debre, P. et al. (1998). Susceptibility of peripheral blood mononuclear cells to apoptosis is correlated to plasma HIV load. AIDS Research and Human Retroviruses 17, 41923.
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Cottrez, F., Manca, F., Dalgleish, A. G., Arenzana-Seisdedos, F., Capron, A. & Groux, H. (1997). Priming of human CD4+ antigen-specific T cells to undergo apoptosis by HIV-infected monocytes. Journal of Clinical Investigation 99, 25766.
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Received 19 April 2000; returned 30 August 2000; revised 18 September 2000; accepted 11 October 2000