Chicken BAFFa highly conserved cytokine that mediates B cell survival
Kirsten Schneider1,
Sonja Kothlow2,
Pascal Schneider3,
Aubry Tardivel3,
Thomas Göbel2,
Bernd Kaspers2 and
Peter Staeheli1
1 Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, University of Freiburg, 79104 Freiburg, Germany 2 Institut für Tierphysiologie, University of Munich, 80539 Munich, Germany 3 Institute of Biochemistry, University of Lausanne, 1066 Epalinges, Switzerland
The first two authors contributed equally to this work
Correspondence to: P. Staeheli; E-mail: staeheli{at}ukl.uni-freiburg.de
Transmitting editor: M. Reth
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Abstract
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Members of the tumor necrosis factor (TNF) family play key roles in the regulation of inflammation, immune responses and tissue homeostasis. Here we describe the identification of the chicken homologue of mammalian B cell activating factor of the TNF family (BAFF/BLyS). By searching a chicken EST database we identified two overlapping cDNA clones that code for the entire open reading frame of chicken BAFF (chBAFF), which contains a predicted transmembrane domain and a putative furin protease cleavage site like its mammalian counterparts. The amino acid identity between soluble chicken and human BAFF is 76%, considerably higher than for most other known cytokines. The chBAFF gene is most strongly expressed in the bursa of Fabricius. Soluble recombinant chBAFF produced by human 293T cells interacted with the mammalian cell-surface receptors TACI, BCMA and BAFF-R. It bound to chicken B cells, but not to other lymphocytes, and it promoted the survival of splenic chicken B cells in culture. Furthermore, bacterially expressed chBAFF induced the selective expansion of B cells in the spleen and cecal tonsils when administered to young chicks. Our results suggest that like its mammalian counterpart, chBAFF plays an important role in survival and/or proliferation of chicken B cells.
Keywords: B lymphocyte, chicken, cytokine, hematopoiesis
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Introduction
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Members of the tumor necrosis factor (TNF) family and their receptors are important regulators of the immune system [reviewed in (1)]. B cell activating factor of the TNF family (BAFF, also known as BLyS, TALL-1, THANK, zTNF4 or TNFSF13b) (25) is a new member of the TNF family of cytokines that plays a major role in B cell survival, proliferation and differentiation (6). Ligands of the TNF superfamily are type II single transmembrane proteins that form biologically active trimers (7). The TNF family defining amino acids are located in the C-terminal extracellular receptor-binding domain of the uncleaved precursor. BAFF has 33% amino acid identity to APRIL (a proliferating inducing ligand) and
20% to TNF-
(2). The C-terminal extracellular domain can be cleaved by a furin-like protease resulting in release of the biologically active soluble cytokine (2). BAFF is produced by macrophages, dendritic cells and some T cells (2,3,8).
BAFF can bind three receptors of the TNF receptor family, TACI (transmembrane activator, calcium modulator and cyclophilin ligand interactor), BCMA (B cell maturation antigen) and BAFF-R (BAFF receptor). Interestingly, BCMA and TACI also serve as receptors for APRIL (912). TACI is expressed on B cells and activated T cells, whereas BAFF-R and BCMA are expressed mainly or exclusively on B cells (1316). Recent data suggest that BAFF-R may be the primary receptor responsible for BAFF-mediated function (13). BAFF promotes B cell survival (17) and, together with signals from the BCR, BAFF also leads to proliferation of B lymphocytes (25). Treatment of mice with recombinant BAFF increased the B cell frequency in the spleen and elevated the serum Ig levels (3,18). The same effects were seen in BAFF transgenic mice (12,19,20). In addition these mice showed signs of autoimmune diseases such as systemic lupus erythematosus (SLE) and, at a later stage, Sjögrens syndrome (SS) (12,1921), suggesting that BAFF is a key player in human autoimmune disease. Indeed, some SLE and SS patients have increased levels of BAFF in the serum (2123), and BAFF null mice display dramatically reduced peripheral B cell numbers and Ig levels (24,25). Treatment of animals with a decoy receptor for BAFF reduced the mature B cell pool and prevented disease in mouse models of SLE and collagen-induced arthritis (12,2628).
Little information is available on TNF family members in the chicken. The coding sequences of the chicken homologues of CD40 ligand (GenBank accession no. AJ243435), TRAIL (TNF-related apoptosis inducing ligand; GenBank accession no. AY057941) and Fas (GenBank accession no. AF296874) have been reported. Fragments of other putative family members can be found in EST databases, but their identity remains questionable. Here we report the cDNA cloning and biological characterization of the chicken homologue of mammalian BAFF, designated chBAFF.
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Methods
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Animals
H.B19 (B19/B19 haplotype) chickens were raised at the Institute of Animal Physiology, University of Munich. Fertilized eggs of M11 (B2/B2 haplotype) chickens were obtained from the Institute for Animal Science, Mariensee (29) and hatched at the Institute of Animal Physiology, Munich. M11 birds were used for the experiments described in Fig. 6(B and C).

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Fig. 6. chBAFF promotes the survival of splenic B cells in culture. (A) Splenocytes were cultured for 72 h with 5% Flag-chBAFF-containing medium or with 5% medium of control cells transfected with the empty plasmid and analyzed by flow cytometry. Cells were stained with PI to determine the percentage of dead cells, and with mAb to the B cell-specific marker Bu-1b and the T cell marker CD3. (B) Splenocytes (5 x 106 cells) were treated with different concentrations (100.0001%) of Flag-chBAFF-containing medium or with 10% medium of control cells transfected with the empty plasmid. After 48 h, the number of viable B cells was determined by Trypan blue exclusion and FACS analysis. Data represent mean ± SD of three independent experiments. (C) Kinetics of cell survival. Purified splenic B cell preparations (5 x 106 cells, purity >93%) were cultured for the indicated times in the presence of 5% Flag-chBAFF-containing medium or 5% medium of control cells transfected with the empty plasmid. Data represent mean ± SD of three independent experiments. (D) CFSE-labeled splenocytes were cultured for 48 h in the presence or absence of chBAFF as described in (A) or as positive control with ConA, and analyzed by flow cytometry. Cells were stained with 7-AAD, and two color FACS analysis was performed on the living cell population.
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Cells
Human embryonic kidney 293T cells were maintained in DMEM supplemented with 10% FBS. Chicken splenocytes, cells of the bursa of Fabricius, the cecal tonsils and the thymus were separated by density centrifugation on Ficoll-Paque (Amersham Pharmacia Biotech, Freiburg, Germany). Peri pheral blood lymphocytes were prepared by slow speed centrifugation as previously described (30). Cells were maintained in RPMI 1640 medium with Glutamax (Gibco, Paisley, UK) supplemented with 10% FCS at 40°C in a CO2 incubator.
Cloning of chBAFF cDNA
The overlapping bursal EST database clones dkfz426_2f7r1 and riken1_33a18r1 (sequence data available at http://swallow.gsf.de/dt40Est.html) contained the sequence information of the 3' and 5' portion of the chBAFF cDNA respectively. PCR with the oligonucleotides 5'-AGCAACGA GTAACATGGATTGCAC-3' and 5'-GCTTCAGAGGAGTCTAA CTGCAC-3' using a 855-23 T cell cDNA library as template (31) resulted in the amplification of the complete open reading frame of chBAFF. The PCR product was cloned into pcDNA3.1 (Invitrogen, Karlsruhe, Germany). The sequence has been assigned the GenBank accession no. AF506010.
RNA isolation and northern blot analysis
RNA was derived from various organs of 3-week-old chickens using TRIzol reagent (Gibco, Karlsruhe, Germany) according to the manufacturers protocol. RNA samples (15 µg per lane) were size-fractionated by electrophoresis through agarose gels containing 4% formaldehyde using standard procedures before blotting onto nylon membranes and hybridization with the indicated cDNA probes that were radiolabeled with 32P.
Expression of recombinant chBAFF
For expression of chBAFF in eukaryotic cells, PCR was performed on cDNA of the chicken T cell line 855-23 (31) with primers 5'-CAAATGCATTCTATTGTCAACGCAGAAGAA AC-3' and 5'-CCGGAATTCTCAGAGGAGTCTAACTGCAC-3' that introduced unique NsiI and EcoRI restriction sites near the ends of the fragment. The resulting PCR product was digested with NsiI and EcoRI, and ligated behind the hemagglutinin signal peptide and Flag sequence of a modified PCR-3 vector (2). 293T cells were transfected with the resulting construct encoding the 152 C-terminal amino acids of chBAFF or with empty vector using Metafectene (Biontex, Munich, Germany) according to the manufacturers protocol. At 72 h post-transfection, the supernatants were harvested and cleared of cell debris by centrifugation. For expression in Escherichia coli, PCR was performed with primers 5'-CGCGGATCCTCTATTGTCAACGCAGAAGAAACA-3' and 5'- CCCAAGCTTTCAGAGGAGTCTAACTGCACC-3' that introduced unique BamHI and HindIII restriction sites. The resulting DNA product was cloned between the BamHI and HindIII restriction sites of the prokaryotic expression vector pQE9 (Qiagen, Hilden, Germany). Expression of this construct in E. coli strain M15 yielded His-chBAFF with an N-terminal extension consisting of the peptide Met-Arg-Gly-Ser-(His)6-Gly-Ser. His-chBAFF was purified by affinity chromatography on a nickel chelate agarose column. Purification conditions were essentially as described previously for chicken IFN-
(31). Induction was at OD600 = 0.5 for a period of 1 h using an isopropylthiogalactose concentration of 1.5 mM.
Co-immunoprecipitation and western blotting
To detect the interaction between BAFF and various TNF receptors, 293T cells were transfected with eukaryotic expression plasmids for either Flag-chBAFF, Flag-huBAFF (2), Flag-huEDA1, huBCMA:Fc, muBCMA:Fc, huTACI:Fc, muTACI:Fc or huBAFFR:Fc (13,32). Cell culture supernatants were collected at 72 h post-transfection. Samples of the various receptor:Fc fusion proteins (
500 ng) were mixed with Flag ligands (200 ng), adjusted to 1 ml with PBS and incubated for 2 h at 4°C on a wheel with 5 µl of Protein ASepharose (Amersham Pharmacia Biotech, Freiburg, Germany). Beads were harvested, loaded in empty mini-columns, washed 3 times with 200 µl of PBS, eluted with 15 µl of 0.1 M citrate NaOH, pH 2.7, neutralized and analyzed by western blotting with anti-Flag M2 antibody (Sigma, Taufkirchen, Germany). Membranes were subsequently reprobed with horseradish peroxidase-coupled goat anti-human IgG antibodies.
Receptorligand interaction ELISA
Recombinant receptor:Fc fusion proteins and Flag-tagged soluble ligands were produced in 293T cells as described above. Specific receptorligand interactions were detected by ELISA as described (13). Briefly, plates were coated with mouse anti-human IgG. Plates were then incubated sequentially with receptor:Fc, Flag ligands, biotinylated anti-Flag M2 mAb, horseradish peroxidase-coupled streptavidin and o-phenylenediamine. To ascertain the functionality of the recombinant receptors when no specific ligand was available, 100 µl of horseradish peroxidase-coupled rabbit anti-human IgG (1:4000) was used to demonstrate coating of receptor:Fc.
In vitro survival assay
Cells were cultured in standard RPMI 1640 medium with Glutamax supplemented with 10% FCS at 5 x 106 cells/well in 24-well plates at 40°C. Supernatants of 293T cells transfected with either the Flag-chBAFF expression construct or with empty vector were added to the culture medium at a final concentration of 5% or at the indicated concentrations for doseresponse experiments. Mammalian BAFF was used at 2 µg/ml, a concentration previously shown to induce maximal B cell responses in the murine system (17). Absolute numbers of viable cells in the cultures were determined by Trypan blue exclusion and the frequency of living B cells [L chain+/propidium iodide (PI)] was then analyzed by flow cytometry as described below. The number of viable B cells was eventually calculated from these data. To investigate whether chicken BAFF induces proliferation of B-lymphocytes, purified splenocytes were resuspended in PBS containing 2% chicken serum and 2.5 µmol/l carboxy-fluorescein diacetate succinimidyl ester (CFSE) stock solution was prepared as described by the manufacturer (Molecular Probes, Leiden, The Netherlands) and incubated at 37°C for 30 min in a shaking water bath. Labeled cells were washed twice in warm RPMI 1640, adjusted to a final concentration of 1x107 cells/ml and cultured in the presence or absence of chBAFF as described above. As a control, cells were stimulated with 10 µg/ml of concanavalin A (ConA). After 48 h cells were stained with antibody AV-20 specific for the BU-1 antigen (Southern Biotechnology Associates, Birmingham, AL) and goat anti-mouse-PE (Southern Biotechnology Associates). Dead cells were stained with 7-amino-actinomycin D (&-7-AAD, Sigma) at 25 µg/ml, and FACS analysis was performed on the living cell population. Splenic B cell preparations of >93% purity were obtained by magnetic cell sorting on MACS separation columns LS (Miltenyi Biotec, Bergisch Gladbach, Germany) using a chicken Ig light chain (L chain)-specific antibody (Southern Biotechnology Associates, Birmingham, AL), anti-mouse IgFITC (Sigma) and anti-FITC Micro Beads (Miltenyi Biotec).
In vivo activity of chBAFF
Young chicks (3 days old) were given i.p. injections of either purified recombinant His-chBAFF or His-MxA (2 mg protein/kg body mass twice a day) over a period of 7 days. Spleens were removed and the spleen/body weight index was calculated. Lymphocytes from the spleen and cecal tonsils were separated on Ficoll (Amersham Pharmacia Biotech), and subjected to analysis by flow cytometry. In order to obtain sufficient numbers of cecal tonsil lymphocytes, organs from all birds in each group were pooled prior to lymphocyte isolation.
Flow cytometry
Staining of the cells for flow cytometric analysis was performed according to standard procedures. Cells were stained with a mAb against IgM (M1) (33), L chain (Southern Biotechnology Associates, Birmingham, AL), Bu-1b (34), CD3 (CT3) (35), CD4 (36), CD8 (37) and TCR2 (38) followed by an anti-mouse IgFITC conjugate (Sigma). Staining with 293T supernatants transfected with either an expression construct for Flag-chBAFF or empty vector (dilution 1:10) was followed by staining with anti-Flag mAb (M2) (Sigma) and anti-mouse Igphycoerythrin (PE) conjugate (Southern Biotechnology Associates, Birmingham, AL). For two-color immunofluorescence staining, cells were first incubated with the CD3 or Bu-1b antibodies followed by anti-mouse IgFITC and normal mouse serum. The cells were subsequently stained with Flag-chBAFF, Flag-huBAFF or Flag-muBAFF followed by an anti-Flagbiotin conjugate (Sigma) and streptavidinPE (Southern Biotechnology Associates). Dead cell discrimination for in vitro survival assays was done through staining with PI (2.5 µg/ml). Analysis was performed with a FACScan (Becton Dickinson, Heidelberg, Germany) using CellQuest software.
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Results
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Identification of a cDNA for chBAFF
Analysis of a chicken bursal EST database (http://swallow.gsf.de/dt40Est.html) indicated that clone dkfz426_2f7r1 and clone riken1_33a18r1 contained sequence information of a polypeptide with high similarity to human and murine BAFF. A cDNA encoding the complete protein was obtained by combining the sequence information of the two overlapping EST clones (Fig. 1). chBAFF comprises 271 amino acids, contains a predicted transmembrane domain (TMD) and also a putative furin protease cleavage site like its mammalian counterparts. Sequence identity at the amino acid level between the soluble parts of chBAFF (starting with a serine residue at position 136) and human or murine BAFF was 76 and 70% respectively.

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Fig. 1. Comparison of BAFF protein sequences from chicken, man and mouse. Sequences of chBAFF (GenBank accession no. AF506010), huBAFF (GenBank accession no. AF116456) and muBAFF (GenBank accession no. AF119383) were aligned using the CLUSTAL method. Amino acid residues are represented by the one-letter code, with columns indicating identical amino acids. Dots indicate residues showing similarity, defined as acidic (D or E), basic (H, K or R), hydrophobic (A, F, I, L, M, P, V or W) or polar (C, G, N, Q, S, T or Y). The TMD is shaded and the furin cleavage site is boxed.
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The expression profile of chBAFF was assessed by northern blot analysis of RNA from various chicken tissues and of the chicken T cell line 855-23 (31) using a radiolabeled PCR fragment derived from the chBAFF sequence. This cytokine seemed to be encoded by an mRNA of
3 kb length that was mainly found in the bursa of Fabricius. The transcript was also present in cells of the 855-23 T cell line (Fig. 2).

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Fig. 2. chBAFF gene expression profile. (A) chBAFF cDNA was used to probe a northern blot containing RNA from various chicken tissues and of the chicken T cell line 855-23 (31). (B) Re-probing of the membrane was performed with chicken GAPDH cDNA (41) to estimate the relative amounts of RNA loaded in each lane. The gel positions of the 28S rRNA and 18S rRNA are indicated.
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Recombinant chBAFF binds to mammalian BAFF-R
Three different receptors of the TNF receptor family, i.e. TACI, BCMA and BAFF-R, can bind mammalian BAFF. The chicken homologues of these receptors have not been identified to date. Therefore, by co-immunoprecipitation analysis we tested whether chBAFF would bind to the mammalian BAFF-R. To generate an appropriate chBAFF expression construct, we performed PCR on cDNA from the chicken T cell line 855-23 (31) to amplify a fragment that encoded the putative soluble form of chBAFF containing all amino acids located downstream of the furin cleavage site. To produce a secreted form of chBAFF, the resulting cDNA fragment was cloned downstream of the hemagglutinin signal peptide and the Flag sequence of a modified version of the PCR-3 vector which permits efficient expression of cDNAs in eukaryotic cells (32). Supernatants of transfected 293T cells containing either Flag-chBAFF or Flag-huBAFF were used to perform co-immunoprecipitations with soluble fusion proteins consisting of the Fc domain of human IgG1 fused to the N-terminal extracellular domains of huTACI, muTACI, huBCMA, muBCMA and huBAFF-R (huTACI:Fc, muTACI:Fc, huBCMA:Fc, muBCMA:Fc or huBAFFR:Fc) respectively. The receptor:Fc fusion protein of the human TNF receptor family member ectodysplasin A receptor (EDAR:Fc) which binds the human TNF family ligand EDA1 and conditioned medium from 293T cells transfected with a expression construct for huEDA1 served as controls. Complexes were precipitated with Protein ASepharose beads, and bound proteins were analyzed by western blotting using antibodies to human IgG (Fig. 3, top) and a mAb against the Flag epitope (Fig. 3, bottom). chBAFF strongly interacted with human and murine TACI:Fc, BCMA:Fc and human BAFF-R:Fc, and did not interact with human EDAR:Fc (Fig. 3). To further demonstrate the specificity of chBAFF, we tested its ability to bind several additional human and mouse TNF family receptors in a receptorligand interaction ELISA. chBAFF and huBAFF interacted with human and mouse TACI, BCMA and BAFF-R, and both proteins did not bind to the other TNF family receptors that we tested (Fig. 4). The functionality of the various plate-coated recombinant receptor:Fc fusion proteins was demonstrated using the cognate ligands (data not shown). When no specific ligand was available, horseradish peroxidase-coupled rabbit anti-human IgG was used to demonstrate successful coating of receptor:Fc (data not shown).

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Fig. 3. chBAFF binds mammalian BAFF-R. The receptor:Fc fusion proteins huTACI:Fc, muTACI:Fc, huBCMA:Fc, muBCMA:Fc, huBAFF-R:Fc and huEDAR:Fc were mixed with supernatants of 293T cells transfected with expression plasmids encoding either Flag-huBAFF, Flag-chBAFF or Flag-huEDA, precipitated with Protein ASepharose and detected by western blotting using anti-Flag mAb M2 (bottom panel). The presence of receptor:Fc proteins was visualized with antibodies to human IgG (top panel). IP: immunoprecipitation; WB: western blot.
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Fig. 4. chBAFF is a ligand for the mammalian receptors TACI, BCMA and BAFF-R. Binding of Flag-chBAFF and Flag-huBAFF to various TNF family receptor:Fc fusion proteins was assessed by ELISA. The plates were coated with the indicated receptor:Fc fusion proteins, ligand was added and binding was revealed using anti-Flag M2 mAb.
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chBAFF-R are expressed exclusively on B cells
Mammalian BAFF-R are expressed mainly on B cells, suggesting that chBAFF-R might also be expressed on B cells of lymphoid tissues such as bursa of Fabricius, spleen, cecal tonsils and peripheral blood lymphocytes. Using recombinant Flag-chBAFF as a probe, we determined receptor expression by flow cytometry. We found that most cells of the bursa of Fabricius expressed receptors for chBAFF (Fig. 5A, left). In contrast, thymocytes mostly did not bind Flag-chBAFF (Fig. 5A, right), indicating that chBAFF-R are preferentially expressed on B cells. This conclusion was confirmed by two-color immunofluorescence staining (Fig. 5B). chBAFF-R expression was restricted to the Bu-1b+ B cell population in peripheral blood lymphocytes, spleen cells and cecal tonsils. ChBAFF-R expression was absent from CD3+ cells.

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Fig. 5. Binding of chicken and mammalian BAFF to chicken lymphocytes. Binding of Flag-chBAFF to isolated cells from (A) bursa of Fabricius or thymus and (B) cecal tonsils, spleen or peripheral blood lymphocytes from 7-week-old birds was analyzed by flow cytometry. (C) The ability of flagged huBAFF, muBAFF or chBAFF to recognize binding sites on isolated chicken splenocytes was compared by flow cytometry.
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Since chicken and mammalian BAFF show a high degree of homology, we also investigated whether mammalian BAFF binds to chicken lymphocytes. As shown in Fig. 5(C), selective binding of human and murine BAFF to chicken B cells could be demonstrated. The staining patterns and the staining intensities were comparable to those observed with chBAFF.
chBAFF promotes the survival of splenic B cells
Mammalian BAFF is a potent survival factor for B cells. To test whether this also holds true for the chicken system, splenocytes were isolated and treated for 72 h with either recombinant Flag-chBAFF or control 293T cell supernatant. Analysis with a mAb specific for the B cell-surface marker Bu-1b combined with PI staining revealed that the B cell frequency in BAFF-treated samples was
3-fold higher compared to the controls, whereas the frequency of CD3+ T cells was not affected (Fig. 6A). To further investigate this effect, splenocytes were cultured in the presence of different concentrations of chBAFF. After 48 h, the number of viable B cells was determined. We observed a dose-dependent response to cytokine treatment. Saturation was reached if the BAFF-containing supernatant was used at concentrations >1% (Fig. 6B). To exclude the possibility that secondary effects influenced the results in our mixed cell preparations, several experiments were performed using splenic B cells enriched to > 93% purity. Although cell death occurred rapidly in all cultures, the absolute numbers of living cells were higher in Flag-chBAFF-treated cultures compared to control cultures at 24, 48 and 72 h (Fig. 6C). On average, viable B cell numbers were nearly 3-fold higher after 48 h and
5-fold higher after 72 h, strongly indicating that recombinant chBAFF promoted B cell survival in these cultures. To definitely prove that this effect was not a consequence of B cell proliferation, we cultured CFSE-labeled splenocytes with chBAFF or ConA. ConA-stimulated cultures showed evidence for two to three rounds of cell division after 48 hours. Under these conditions no cell proliferation was observed in the chBAFF-treated culture or in the untreated control, although the number of living B cells increased from 13.5% to 26.5% in response to chBAFF (Fig. 6D). The almost threefold decrease in the number of living B cells in the ConA-treated culture can probably be explained by the strong increase of T cells.
Since human and mouse BAFF efficiently bound to chicken B cells, we next determined whether they are also biologically active on chicken cells. Splenocytes were treated with flagged human or mouse BAFF (2 µg/ml) for 48 h before the number of viable B cells was determined. Cells treated with Flag-chBAFF (5%) or control supernatant (5%) served as positive and negative controls. We found that the mammalian BAFF preparations were nearly as potent in preventing premature death of chicken splenocytes as chBAFF (Table 1).
Increased B cell numbers in chicks treated with recombinant chBAFF
To determine the in vivo activity of chBAFF, 3-day-old chicks (seven animals per group) were injected i.p. twice a day for 7 days with 2 mg/kg body weight of E. coli-derived chBAFF (His-chBAFF) or with the same amount of control protein (His-MxA). The spleen/body weight index was significantly increased (P < 0.05, in the Wilcoxon test and Students t-test) in chBAFF-treated birds (Table 2), indicating an increase of total lymphocyte numbers in the spleen. Flow cytometric analysis of spleens and cecal tonsils with various mAb revealed that the percentage of B cells, but not T cells, was
2-fold higher in chBAFF-treated animals as compared to control animals (Table 3). The Ig light chain (L chain) staining pattern of a representative pair of chBAFF- and control-treated animals is shown in Fig. 7. Since splenic lymphocyte numbers and B cell frequencies were higher in chBAFF-treated animals, it can be concluded that chBAFF enhances survival or induces selective proliferation of B cells in spleen and cecal tonsils of cytokine-treated animals.

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Fig. 7. Increased B cell numbers in chicks treated with recombinant chBAFF. Effect of i.p. administration of His-chBAFF or control protein (His-MxA) on the frequency of various cell types in the spleen and cecal tonsils of 10-day-old chicks at 7 days post-initiation of treatment. Cells were stained with the indicated mAb and analyzed by flow cytometry. A representative data set of spleen and cecal tonsils from a pair of chBAFF- and control-treated animals stained for the Ig light chain (L chain) is shown.
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Discussion
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Here we report the identification and biological characterization of the chicken homologue of mammalian BAFF. By analyzing the BLAST search results of two chicken EST databases, we identified overlapping cDNA clones that code for the complete open reading frame of chBAFF. Like its mammalian counterparts, chBAFF exhibits a type II transmembrane protein organization. Mammalian BAFF is processed by a furin-like protease after a characteristic sequence motif which results in the release of biologically active soluble protein (2). chBAFF contains a putative furin cleavage site at the corresponding position, indicating that it might be processed similarly. The predicted soluble forms of chicken and mammalian BAFF are surprisingly similar. At the amino acid level, soluble chBAFF is 70 and 76% identical to mouse and human BAFF respectively. Most other avian cytokines show <30% identity to their mammalian counterparts (39).
The high sequence conservation suggested that chBAFF might bind to mammalian BAFF-R. By performing appropriate co-immunoprecipitation experiments and by using a receptorligand interaction ELISA we were able to demonstrate that this was indeed the case. Like human BAFF, chBAFF bound specifically to the mammalian receptors TACI, BCMA and BAFF-R. The receptors for BAFF in mammals are expressed on B cells and on some T cells (1214). Our flow cytometric analyses revealed that Flag-tagged chBAFF bound a population of cells which was abundantly present in chicken spleen, bursa of Fabricius, peripheral blood lymphocytes and cecal tonsils, but which was virtually absent in the thymus. Since
98% of the lymphocytes present in the bursa of Fabricius are B cells, our data strongly suggested that the receptors for BAFF in the chicken are expressed predominantly if not exclusively on B cells, which was confirmed by two-color immunofluorescence staining.
As expected from the known activity of mammalian BAFF, chBAFF did not induce B cell proliferation but was clearly able to prolong the survival of splenic B cells in cell culture. Mammalian BAFF also bound to chicken B cells and promoted their survival, indicating functional cross-reactivity between mammalian and avian BAFF. chBAFF further promoted the specific expansion of the B cell population in the spleen and cecal tonsils of young chicks that were treated with recombinant cytokine. Further studies are needed to determine whether the in vivo expansion of B cells resulted from enhanced survival or from increased proliferation of this lymphocyte population. In contrast to spleen and cecal tonsils, the number of B cells in the bursa of Fabricius of treated chicks was not increased and the size of the organ remained unchanged. This differential response to chBAFF of B cells in various lymphoid organs is not really surprising. Indeed, studies with transgenic and knockout mice have clearly demonstrated that mammalian BAFF is critical for B cell maturation in peripheral lymphoid organs, but is not required for B cell development in the bone marrow, despite the fact that BAFF mRNA is moderately expressed in this compartment (3,17,24). In birds, early development of B cells does not take place in the bone marrow, but in the bursa of Fabricius (40). By analogy with the mammalian system, the first steps of B cell development in the chicken are not expected to be dependent on BAFF. However, in view of the fact that the chBAFF gene is expressed most strongly in the bursa, it may well be that BAFF is important for bursal B cell survival. chBAFF could play a role in a late B cell differentiation step, shortly before the B cells exit from the bursa. Since administration of exogenous BAFF did not positively affect the number of B cells in the bursa, at first glance, this result seems to argue against this view. It is possible, however, that the level of endogenous BAFF is already very high in the bursa and that administration of exogenous BAFF has no additive effect simply because the BAFF-R on bursal B cells are already saturated with ligands.
In mammals, BAFF is expressed by cells of myeloid origin and, to a lesser extent, by T cells, but not by B cells (2,3,8). Since spleen did not contain detectable levels of BAFF transcripts, mature chicken B cells cannot be the major producers of BAFF. Thus, most likely, BAFF is synthesized by either bursa stromal cells or immature B cells. To further investigate the role of this cytokine in bursal B cell development, the activity of endogenous chBAFF needs to be blocked in vivo. Since the generation of knockout birds is still impossible, alternative strategies aimed at neutralizing this cytokine with antagonistic antibodies or with soluble decoy receptors such as BAFF-R:Fc should be considered. Results presented here (Figs 3 and 4) strongly indicate that BAFF-R:Fc has the potential to neutralize the activity of chBAFF.
In summary, by identifying chicken BAFF as well as suitable decoy receptors we paved the way for new experimental approaches aimed at a better understanding of B cell development in the chicken. Furthermore, our work is the first functional characterization of a chicken TNF family member.
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Acknowledgements
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We thank Drs C. Ambrose and J. Thompson (Biogen, Boston, MA) for providing the cDNA for BAFF-R, and Dr E. Duda for help with the in vivo experiment. This work was supported by grants from the Deutsche Forschungsgemeinschaft, the European Community, the Swiss National Research Foundation and Intervet Int., BV, Boxmeer, The Netherlands.
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Abbreviations
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APRILa proliferating inducing ligand
BAFFB cell activating factor of the TNF family
BAFF-RBAFF receptor
BCMAB cell maturation antigen
PEphycoerythrin
PIpropidium iodide
SSSjögrens syndrome
SLEsystemic lupus erythematosus
TACItransmembrane activator, calcium modulator and cyclophilin ligand interactor
TMDtransmembrane domain
TNFtumor necrosis factor
TRAILTNF-related apoptosis inducing ligand
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