Calcium responses elicited in human T cells and dendritic cells by cell–cell interaction and soluble ligands

Monica Montes, Dorian McIlroy, Anne Hosmalin and Alain Trautmann

The editors regret that Figs. 1 and 3GoGo in this article were not reproduced in colour as the authors intended. The figures, with their legends, are reproduced below.



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Fig. 1. Interactions between T cells and DCs. A,B: response of a T cell interacting with an Ag-pulsed DC. A: Sequence of events observed when a fura-2 loaded T cell reaches a DC. Image interval: 48 s. B: Two examples of single T cell Ca2+ responses. At time zero, the T cells appeared in the focal plane of the DCs. The dotted line corresponds to the cell shown in A. C: A T cell can interact with more than one DC. Top: Time zero of the interaction. Bottom: 9 minutes later. Left: Fluorescence of the fura-2-loaded cell used to visualise its shape. Right: Superimposition of Nomarski and Ca2+ images (same colour coding as in A). D,E: Response of a T cell interacting with a DC in the absence of Ag. D: Sequence of events observed with an image interval of 48 s. E: Three examples of single T cell Ag-independent Ca2+ responses.

 


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Fig. 3. Ca2+ responses can also be triggered in DCs by T cell contact. A: Example of Ca2+ responses observed both in the T cell (continuous line) and in the Ag-pulsed DC (dotted line). B: Sequence of images (2 min. interval) showing a Ca2+ response in the smaller T cell and then in the DC, following cell–cell interaction. C: Example where a Ca2+ response was observed only in the T cell (same symbols as in A). D: Sequence of images (2 min. interval) showing a Ca2+ response only in the T cell following T–DC interaction. Note the marked flattening of the T cell in the last image. The fluorescence of the flattened area is too dim to be detected.