Department of Mammalian Genes Expression, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Vídeská 1083, 142 20 Prague 4, Czech Republic
Correspondence to: P. Dráber; E-mail: draberpe{at}biomed.cas.cz
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Abstract |
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Keywords: basophil, IgE receptor, Lyn kinase, mast cell, Syk kinase
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Introduction |
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An alternative model has recently been suggested, which postulates that Lyn is not pre-associated with FcRI, but instead is sequestered into specialized detergent-resistant membrane microdomains, called lipid rafts, which are enriched in glycosphingolipids, cholesterol, glycosylphosphatidylinositol (GPI)-anchored proteins, Src family kinases and some other signal-transducing molecules (5,6). Aggregation brings Fc
RI into these lipid rafts where it is phosphorylated by lipid raft-associated Lyn kinase (7,8). Although the role of lipid rafts in signal transduction was confirmed for other immunoreceptors on T cells (911) and B cells (12), molecular mechanisms underlying the integration of lipid raft components with immunoreceptors, as well as their composition, are only incompletely explained (for review, see 13).
A role for lipid rafts in the initiation of mast cell activation was suggested by earlier studies indicating that the aggregation of a lipid raft component, the Thy-1 glycoprotein (gp), induced cell activation events which resembled FcRI-mediated activation events, although they were lower in extent and were observed even in mutant cells defective in the expression of surface Fc
RI (1417). Furthermore, it has been observed that glycosphingolipid-specific mAb 2B5 induces the release of secretory components and redistribution of fluorescently labeled IgE similar to that produced by antigen-mediated aggregation of Fc
RI (18). Because this effect was observed only in IgE-sensitized cells, it seems that the activation induced by 2B5 mAb is mediated via aggregated Fc
RIIgE complexes (18). Different results were obtained with the AA4 mAb, which binds an
-galactosyl derivative of ganglioside GD1b (19,20). Although this mAb induced tyrosine phosphorylation of Fc
RI ß and
subunits, Syk kinase, and other substrates, its activity was blocked when Fc
RI was saturated with IgE or when F(ab')2 fragments of AA4 mAb were used, suggesting that direct aggregation of Fc
RI and GD1b is involved (21).
In an attempt to elucidate the complexity of lipid rafts and their role in mast cell activation we prepared mAb against lipid rafts from RBL-2H3 cells and characterized the target antigens. In this study we describe a novel lipid raft-associated gp, TEC-21, which belongs to the urokinase plasminogen activator receptor (uPAR)/Ly-6/snake neurotoxin family. Its expression in normal tissues seems to be restricted to testes and no evidence of expression in primary mast cells was found. Nevertheless, because of its constitutive inclusion in RBL-2H3 lipid rafts, it provided us the opportunity to study interplay between FcRI- and lipid raft-mediated signaling. We found that antibody-mediated aggregation of TEC-21 gp induced cell activation events and we analyzed whether Fc
RI was involved in this process. We also studied the association of TEC-21 gp with Lyn kinase by confocal microscopy and the changes in this association after aggregation of the TEC-21 gp. Our data indicate that aggregated TEC-21, contrary to some other lipid raft components, induced activation independently of Fc
RI.
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Methods |
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To prepare TEC-21 mAb, BALB/c mice were immunized with pooled lipid rafts isolated from RBL-2H3 cells lysed in 1% Brij 96 and fractionated by sucrose density-gradient ultracentrifugation as described (25). Hybridoma cells were obtained after fusion of SP02 mouse myeloma cells with spleen cells of immunized mice using standard procedures (26). A mouse mAb isotyping kit (Sigma) was used to determine the Ig isotype. TEC-21 mAb was isolated from culture supernatant or ascitic fluid on a column of UltraLink-immobilized Protein A beads (Pierce). Antibodies isolated by both these procedures had similar properties. F(ab')2 fragments were obtained by standard procedures (27).
Flow cytofluorometry analysis
Cells (106) were stained with saturating concentrations of mAb followed by FITC-labeled anti-mouse IgG. The cells were evaluated by flow cytofluorometry using a FACScan (Becton Dickinson, Mountain View, CA). In some experiments the cells were stained after pretreatment with phosphatidylinositol-specific phospholipase C (PI-PLC) as described (25).
Ca2+ measurements
Cytoplasmic Ca2+ responses were measured with Fura-2 fluorescence probe as described (28). Briefly, cells were harvested and sensitized or not with biotin-labeled TEC-21 mAb (5 µg/ml) or IgE (IGEL b4 1 ascites diluted 1:1000). After 30 min the cells were washed in BSS (20 mM HEPES, pH 7.4, 135 mM NaCl, 5 mM KCl, 1.8 mM CaCl2, 1 mM MgCl2 and 5.6 mM glucose) supplemented with 0.1% BSA and 2.5 mM probenecid, and resuspended at a concentration of 5x106/ml in BSS/0.1% BSA/probenecid containing 2 µM Fura-2-AM (Molecular Probes, Eugene, OR). After incubation for 40 min at 37°C, the cells were washed in BSS/BSA and the concentrations of free intracellular Ca2+ ([Ca2+]i) measured in a luminescence spectrometer LS-50B (Perkin-Elmer, Beaconsfield, UK) with excitation wavelengths 340 and 380 nm, and with constant emission at 510 nm. The values of [Ca2+]i were calculated using the ICBC Calibration Perkin-Elmer Fluorescence Winlab program (Perkin-Elmer).
Immunoaffinity purification and partial amino acid sequencing
RBL-2H3 cells in monolayer cultures were harvested and suspended in BSS/0.1% BSA. Cells were centrifuged and resuspended in ice-cold PBS containing 0.2% saponin. After 10 min on ice the cells were centrifuged at 4000 g for 5 min and resuspended at a concentration 108 cells/ml in ice-cold lysis buffer (25 mM Tris, pH 8.0, 140 mM NaCl, 2 mM EDTA, 1 mM Na3VO4, 1 mM PMSF, 5 µg/ml aprotinin and 2 µg/ml leupeptin) supplemented with 1% NP-40. After 30 min on ice the lysate was centrifuged at 12,000 g for 10 min. Postnuclear supernatants from 109 cells were precleared with Sepharose CL-4B (Amersham Pharmacia Biotech, Uppsala, Sweden) for 1 h at 4°C, followed by immunoprecipitation with TEC-21 mAb, covalently bound to UltraLink-immobilized Protein A by means of 20 mM dimethyl pimelimidate (Sigma) in 0.2 M sodium borate (pH 9.0). After overnight incubation at 4°C, the beads were washed 4 times with ice-cold lysis buffer with 0.5% NP-40 and the proteins were eluted by boiling in 0.5% SDS. To remove N-linked sugar chains, peptide N-glycosidase F (PNGF; New England BioLabs, Beverly, MA) was used. UltraLink-immobilized Protein A beads with bound mAbTEC-21 gp complexes were boiled in 0.5% SDS. After 5 min the beads were centrifuged and supernatant was supplied with 0.05 M (final concentration) sodium phosphate, 1% NP-40 and 500 U of PNGF per reaction, and incubated at 37°C overnight. After PNGF treatment the samples were reimmunoprecipitated with immobilized TEC-21 mAb for 1 h at 4°C. Proteins were eluted from the beads by boiling in SDS sample buffer without mercaptoethanol, subjected to SDSPAGE and transferred onto a PVDF membrane (Applied Biosystems, Foster City, CA) according to the manufacturer's instructions. The N-terminal amino acid sequence was determined by automated Edman degradation using a Procise protein sequencing system (Perkin Elmer Applied Biosystems, Foster City, CA).
cDNA cloning and sequencing
Based on the N-terminal amino acid sequence and its similarity to the mouse TES101 cDNA sequence (29), a 5' primer (primer I: ACCCTGAGTCTGGAAGAC) was designed. An oligo(dT)primer, TTCTAGAATTCAGCGGCCGCT(TTT)8x, with an XbaI cloning site (underlined) was used for the reverse transcription step using mouse Moloney virus reverse transcriptase (Gibco/BRL, Gaithersburg, MD) and total RNA isolated from RBL-2H3 cells employing acidic phenol extraction reagent (Tri Reagent; Sigma). The cDNA obtained was used in PCR. The PCR-generated DNA fragment of 0.86 kbp was digested with RsaI and XbaI enzymes, and cloned into SmaI/XbaI-digested pBluescript KSII- (Stratagene, La Jolla, CA). The plasmid was amplified in bacteria and four independent clones were sequenced bidirectionally on an automated sequencer ABI Prism 377 (Perkin Elmer Applied Biosystems). After partial cDNA sequencing two other primers were designed [primer II: TGGCCATCAGTCTGCAGCCGAT (PstI site underlined) and primer III: CTCTAGAACCTTAGAGAAGCCATG (XbaI site underlined)] and used for RT-PCR amplification of the remaining part of the mRNA coding region. The PCR product was digested with PstI and XbaI, cloned into PstI/XbaI-digested pBluescript KSII-, and sequenced. Sequences were analyzed by Vector NTI Suite software (InforMax, North Bethesda, MD) and database searches were performed by the BLAST algorithm.
Cell transfection
The coding region of TEC-21 cDNA was amplified by PCR using appropriate primers (AAAGGATCCGCCACCATGGGAGCCTGCCGC and AAGAATTCCGGTGTCCTCTCAAGGGA) with BamHI and EcoRI restriction sites (underlined) and the optimized ribosome binding site (marked in bold letters). For PCR the proofreading Pfu DNA polymerase (Stratagene, La Jolla, CA) was used. The PCR-generated DNA fragments were digested with the appropriate enzymes and introduced into an expression vector pZeo SV2 (Invitrogen, Groningen, Netherlands). COS cells were transiently transfected with the vector containing TEC-21 cDNA or empty vector using the DEAEdextran method as described (30). After 48 h the cells were harvested and the surface expression of the TEC-21 gp was evaluated by flow cytofluorometry (see above).
Antibody radioiodination and direct radioantibody binding assay
Labeling of mAb with 125I (ICN Biomedicals, Costa Mesa, CA) by the chloramine T method and direct radioantibody binding assays were performed as described (31). The number of bound molecules per cell at saturation was extrapolated from Scatchard plots (32). All binding assays were performed at least 3 times in triplicates.
Cell activation, immunoprecipitation and immunoblotting
Cells were harvested, resuspended in culture medium at a concentration of 5x106 cells/ml and sensitized in suspension with TEC-21 mAb or biotin-labeled TEC-21 mAb or TNP-specific IgE. After incubation for 30 min at 37°C in a CO2 incubator, the cells were washed twice in BSS/0.1% BSA and activated at 37°C by exposure to streptavidin (10 µg/ml) or TNP-BSA (500 ng/ml) for different time intervals as specified in Results. Towards the end of the activation period, the cells were briefly centrifuged and the supernatants used for determination of ß-glucuronidase release (28). The cell pellets were resuspended in ice-cold lysis buffer containing 1% Triton X-100 (for Syk or LAT immunoprecipitation or phosphotyrosine immunoblotting) or 0.2% Triton X-100 (for FcRI immunoprecipitation). After 30 min on ice the lysate was centrifuged at 12,000 g for 10 min. Syk, LAT or IgEFc
RI complexes were immunoprecipitated from samples equivalent to 107 cells with the corresponding antibody prebound to UltraLink-immobilized Protein A and immunoblotting was performed as described (22).
Sucrose density-gradient ultracentrifugation and Sepharose 4B gel chromatography
In radioantibody binding experiments, RBL-2H3 cells (2x107) were treated in suspension with various 125I-labeled and unlabeled ligands and aggregating agents as described in Results. The cells were lysed in 0.8 ml of lysis buffer containing 10 mM TrisHCl (pH 8.0), 50 mM NaCl, 10 mM EDTA, 1 mM Na3VO4, 10 mM glycerophosphate, 1 mM PMSF, 5 µg/ml aprotinin, 2 µg/ml leupeptin and 0.06 or 1% (v/v) Triton X-100. After 15 min the lysate was homogenized by passing 5 times through a 27-gauge needle and adjusted to 40% (w/v) sucrose by adding an equal amount of 80% sucrose. Gradient was formed by successive addition of 0.2 ml of 80% sucrose stock to the bottom of a polyallomer tube (13x51 mm; Beckman Instruments, Palo Alto, CA) followed by 0.5 ml of 60% sucrose, 1.5 ml of 40% sucrose (containing the cell lysate), 0.8 ml of 35% sucrose, and 0.5 ml aliquots of 30, 25, 20 and 15% sucrose. Sucrose solutions were prepared by mixing the appropriate amount of the gradient buffer (25 mM TrisHCl, pH 7.5, 125 mM NaCl and 2 mM EDTA) and 80% sucrose. Tubes were centrifuged at 210,000 g for 4 h at 4°C using a SW 55 Ti rotor (Beckman Instruments). Gradients were fractionated into 0.2 ml aliquots withdrawn from the top of the tube and radioactivity in each fraction was determined using a Cobra II -counter (Global Medical Instrumentation, St Paul, MN). The exact sucrose concentration (% w/v) in each fraction was determined by an Abbe refractometer. Gel chromatography on Sepharose 4B minicolumns was carried out as described (16).
Recombinant Semliki Forest virus (SFV) infection and confocal microscopy
SFV gene expression system, pSFV1 and helper pSFV2, was purchased from Gibco/BRL. pSFV1 was further modified to contain a multiple cloning site and a green fluorescent protein (GFP) expression cassette (pSFV-GFP) as previously described (33). Rat LynA cDNA (34) was amplified by PCR using appropriate primers (AAACCTAGGGCCACCATGGGATGTATTAAATCAAAAAGGAAAGAC and AAACCTAGGTGGCTGCTGCTGATACTGCCCTTCCGTGGCAGTGTA) with AvrII restriction sites (underlined) and the optimized ribosome binding site (marked by bold letters). The PCR-generated DNA fragments were digested with AvrII restriction enzyme and then inserted into the AvrII-digested pSFV-GFP. For all PCRs the proofreading Pfu DNA polymerase was used. Fidelity of all PCR products was confirmed by direct sequencing. Generation of SFV and infection of RBL-2H3 cells were performed as previously described (33). SFV infectivity ranged from 60 to 90%.
Immediately after infection the cells were sensitized with biotinIgE (1 µg/ml) or biotinTEC-21 mAb (5 µg/ml), incubated for 3 h at 37°C, washed in BSS/BSA and suspended at 106/ml in BSS/BSA supplemented with Cy3-conjugated streptavidin (2 µg/ml; Pierce). After 2 min at 37°C the cells were washed in PBS, transferred on poly-L-lysine (Sigma; 100 µg/ml)-pretreated cover-slips and centrifuged for 1 min at 200 g. The attached cells were fixed with 4% paraformaldehyde for 15 min at room temperature, washed twice in PBS, dried in air, mounted in 6 µl of ProLong antifade reagent (Molecular Probes) per cover-slip, dried and stored in the dark until use. Control cells were fixed with paraformaldehyde before the exposure to biotin-labeled antibodies and streptavidin. The confocal fluorescence images were taken using the Leica TCS NT/SP confocal system in conjunction with a Leica DM R microscope (Leica Microsystems, Wetzlar, Germany). A chromatically corrected objective x100 (NA 1.4) was used to simultaneously collect green and red images of the cells. Fluorescence bleedthrough was evaluated by separate excitation with blue (488 nm) or yellow (568 nm) laser lines (argon/krypton mix gas laser). Cross-correlation analysis of the co-redistribution of LynGFP constructs with aggregated FcRI or TEC-21 gp complexes was carried out on equatorial images of individual cells using the quantification mode of the Leica TCS NT software (Leica Microsystems). The correlation coefficients were calculated from this analysis as described (35). These values were averaged from each sample for numerical comparison of the degree of co-redistribution.
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Results |
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The expression pattern of TEC-21 gp in rat tissues was examined by immunoblotting. As shown in Fig. 3(A), high expression of TEC-21 gp was found in testicular tissue but no TEC-21 was detectable in brain, bone marrow, thymus, lymph nodes, spleen, liver and heart. Ponceau S staining of membrane-bound proteins indicated that similar amounts of proteins were loaded in all cases (not shown). The apparent molecular mass of TEC-21 gp was lower (3036 kDa) in testes than in RBL-2H3 cells. Immunofluorescence assays indicated that TEC-21 gp was detectable on germinal cells but not on sperm cells (not shown). Interestingly, no TEC-21 was detectable by immunofluorescence on isolated rat peritoneal and pleural mast cells isolated by standard procedure (14) (not shown). The observed expression pattern of TEC-21 was confirmed by RT-PCR analysis, using oligonucleotide primers specific for TEC-21 cDNA (Fig. 3B
). Control RT-PCR with actin-specific oligonucleotide primers confirmed that the difference in TEC-21 mRNA expression in various tissues was not attributable to different amounts of total mRNA used in the assay.
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The binding of TEC-21 mAb alone induced an increase in tyrosine phosphorylation of several endogenous substrates, mainly with apparent molecular masses of 55 and 100 kDa (Fig. 6A
). More extensive TEC-21 cross-linking by biotin-labeled TEC-21 mAb/streptavidin caused more intense tyrosine phosphorylation of several other cellular proteins, i.e. those of
72 kDa. Interestingly, the SDSPAGE profile of tyrosine phosphorylated proteins was similar, but not identical, in cells activated via extensive aggregation of TEC-21 gp and Fc
RI (Fig. 6A
). To determine whether Syk kinase and LAT were among the proteins with increased tyrosine phosphorylation in TEC-21-activated cells, lysates from RBL-2H3 cells activated as above were immunoprecipitated using Syk kinase- and LAT-specific antibody, and the immunoprecipitates were analyzed by immunoblotting with anti-phosphotyrosine mAb (PY-20HRP). Data presented in Fig. 6
(B and C) indicate that TEC-21 mAb alone induced only a weak tyrosine phosphorylation of Syk kinase and LAT. A significant increase in tyrosine phosphorylation of these proteins was observed in cells with extensively aggregated TEC-21. However, the extent of Syk and LAT tyrosine phosphorylation was lower than that seen in cells activated via Fc
RI. Reprobing the membranes with anti-Syk or anti-LAT antibody confirmed that the observed differences were not due to differences in the amount of proteins precipitated. Increased tyrosine phosphorylation of total cellular proteins as well as Syk kinase in TEC-21-activated cells was inhibited by 15-min exposure of the cells to Src kinase inhibitor PP1 (not shown), like in the previously described experiments with Thy-1-activated cells (39).
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Next we studied changes in association of FcRI with lipid rafts after TEC-21 aggregation. It has been previously shown that a small fraction of monomeric IgEFc
RI complexes is associated with lipid rafts as determined by an analysis of sucrose gradient fractionated RBL-2H3 cells, solubilized in lysis buffer with 0.05% Triton X-100. The amount of Fc
RI in lipid raft fractions dramatically increased after Fc
RI aggregation (7,8). As presented in Fig. 7
(A), we confirmed these data using RBL-2H3 cells solubilized in 0.06% Triton X-100-supplemented lysis buffer. Thus, unaggregated [125I]IgEFc
RI complexes were found mostly (80 ± 6%; n = 7) in higher density fractions (3844% sucrose) containing soluble proteins, whereas only 10 ± 4% were found associated with lipid rafts (1533% sucrose). After an aggregation of [125I]IgEFc
RI complexes with anti-IgE, 62 ± 3% (n = 7) of radioactivity was found in lipid raft fractions and only 13 ± 4% in high-density fractions. A significant amount of radioactivity (20 ± 4%) was associated with nuclear remnants localized at fractions 2225.
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Stauffer and Meyer (43) described that fluorescently labeled cholera toxin B co-localized with aggregated FcRI. Cholera toxin B is a marker for glycosphingolipids with strong affinity for GM1. To determine whether aggregation of GM1 would have any effect on the density of monomeric IgEFc
RI complexes we performed another set of experiments in which Fc
RI was labeled with [125I]IgE and GM1 was aggregated with biotin-labeled cholera toxin B and streptavidin. Data presented in Fig. 7
(C) show that the formation of GM1 aggregates is accompanied by transfer of a fraction of [125I]IgEFc
RI complexes (23 ± 1%; n = 3) into lipid raft fractions. Thus, aggregation of two different lipid raft components, TEC-21 and GM1, could have different consequences for the association of Fc
RI with lipid rafts.
The absence of physical and functional association of TEC-21 and FcRI is also supported by experiments assaying tyrosine phosphorylation of Fc
RI ß and
subunits. TEC-21 gp was either dimerized by an exposure of the cells to TEC-21 mAb alone or extensively aggregated by a sequential exposure of the cells to biotin-labeled TEC-21 mAb and streptavidin. Non-activated cells and cells activated by antigen were employed as negative and positive controls respectively. After 5-min exposure to aggregating agents the cells were solubilized in 0.2% Triton X-100 and Fc
RI was immunoprecipitated from postnuclear supernatants by polyclonal anti-IgEIgE complexes immobilized on Protein A beads, except for Fc
RI-activated cells, where immobilized anti-IgE alone was used. Immunoblotting analysis showed that Fc
RI ß and
subunits were tyrosine-phosphorylated in cells activated via Fc
RI but not in cells activated via TEC-21 dimers or extensively aggregated TEC-21 (Fig. 8
). The absence of tyrosine phosphorylation did not reflect lower Fc
RI recovery, since the amount of the Fc
RI ß subunit was comparable in all immunoprecipitates.
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Aggregation of TEC-21 is not accompanied by co-aggregation of Lyn kinase
Confocal microscopy studies showed that aggregated FcRI co-localized with several raft components, including GM1, Thy-1, Lyn and Syk kinase (43,46,47). To find out whether or not TEC-21 is physically associated with Lyn kinase, we studied the co-localization of GFP-labeled Lyn with aggregated TEC-21 by confocal microscopy (Fig. 9
). In cells fixed before TEC-21 staining (top row), both the TEC-21 and LynGFP were homogeneously distributed on respectively the exogenous and endogenous leaflet of the plasma membrane. As expected from the SFV system used (33,46), the LynGFP construct was also localized in the cytoplasm, but was absent in the nucleus. When the cells were fixed after TEC-21 aggregation, Lyn kinase showed no signs of co-localization with aggregated TEC-21. Detailed analysis indicated that the cross-correlation coefficient from 18 cells evaluated attained the value 0.1 ± 0.11 (mean ± SD). Contrary to TEC-21, Lyn kinase exhibited significant co-localization with aggregated Fc
RI. This co-localization was clearly seen on overlays of GFP and Cy3 channels (Fig. 9
bottom line), and the cross-correlation coefficient amounted to 0.49 ± 0.07 (n = 16). Thus, although both TEC-21 and Lyn are localized in lipid rafts, they do not co-localize after TEC-21 cross-linking at physiological temperatures.
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Discussion |
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Sequence analysis revealed that TEC-21 is a gp belonging to the uPAR/Ly-6/snake neurotoxin family. Although the amino acid identity between members of this family is low (30%), the family is defined by the presence of cysteine-rich domains of 7090 amino acids, which contain between eight and 10 cysteine residues, spaced at conserved distances. TEC-21, like some other members of the family, contains one such domain with eight cysteine residues. The highest homology (38% identity) was found with the two cysteine-rich domains of a recently described PRV-1 (48). Searches in mouse DNA and protein databases revealed that TEC-21 exhibited 87% identity to TES101 at the cDNA level (coding region) and 79% identity at the protein level, suggesting that TEC-21 is a rat homologue of mouse TES101. This conclusion is supported by similar expression patterns of mouse TES101 (29) and rat TEC-21 (this study and I. Hálová, unpublished). It should be noted that TES101/TEC-21 homologue is also found in human testes (50% identity; BLAST accession no. AAK27310) and that the human homologue was recently cloned from lung small cell carcinoma (BLAST accession no. AAH01861).
Although the TEC-21 expression seems to be confined in adult rats to testicular tissue, its expression in RBL-2H3 cells could be related to their tumor origin, and, importantly, provides a novel and dominant lipid raft marker in a cell line which has been extensively used for analysis of immunoreceptor signaling and the role of lipid rafts in this process (see Introduction). Antibody mediated aggregation of TEC-21-triggered cell activation events which included enhanced tyrosine phosphorylation of Syk kinase, LAT adaptor and other cellular substrates, elevated [Ca2+]i, and release of a secretory component, ß-glucuronidase. Because TEC-21 is anchored to the plasma membrane via GPI anchor, it is possible that its signaling capacity is mediated via interaction with other signaling molecules with transmembrane and cytoplasmic domains. A likely candidate molecule is FcRI whose aggregation resulted in qualitatively similar activation events as those evoked by aggregation of TEC-21, although of higher magnitude. However, several lines of evidence presented in this study indicate that the TEC-21-mediated activation does not require Fc
RI.
First, aggregation of TEC-21 was not accompanied by tyrosine phosphorylation of FcRI ß and
subunits. Phosphorylation of these subunits by Lyn kinase is the first biochemical event observed in cells activated via Fc
RI. The absence of this step suggests that TEC-21 aggregation does not induce functional interaction of Fc
RI and Lyn. This is true not only for TEC-21 but also for another GPI-anchored protein, Thy-1 (our unpublished data). In this respect activation via GPI-anchored proteins differs from activation mediated via glycosphingolipids (18,42), which are also associated with lipid rafts. Whether this difference reflects heterogeneity among lipid rafts, as suggested by our previous study (25), remains to be determined.
Second, the absence of direct interaction between FcRI and TEC-21 was supported by an assay based on density-gradient fractionation of aggregated and unaggregated Fc
RI and TEC-21 complexes from Triton X-100 lysed cells. In accordance with previous results (7,8), we found that unaggregated IgEFc
RI complexes were localized predominantly in higher density fractions (3844% sucrose). After aggregation, most of the complexes moved into fractions of lower density, corresponding to 1533% sucrose. This movement was observed only when Triton X-100 was used at low concentration (0.06%). Surface TEC-21 gp labeled with [125I]TEC-21 mAb was found mostly in low-density fractions and was resistant to solubilization with 1% Triton X-100. When the cells were solubilized with 1% Triton X-100, 20% of [125I]TEC-21 mAb was found associated with nuclear remnants moving through the 80% sucrose layer. Interestingly, solubilization of the cells in 0.06% Triton X-100 resulted in an association of
44% of TEC-21 with nuclear remnants which had lower density and remained at the 60/80% sucrose interface. Dimerization or extensive aggregation of TEC-21 did not induce any movement of [125I]IgEFc
RI complexes into lower density fractions. This suggests that changes in surface distribution of TEC-21 do not induce any changes in association of Fc
RI with lipid rafts, and that TEC-21 and Fc
RI move independently in the plasma membrane. At present we cannot completely exclude the possibility that these two proteins are somehow physically associated, but the forces that keep them together are weaker than those involved in formation of patches and caps. It should be noted, however, that aggregation of GM1, another lipid raft component, resulted in movement of [125I]Fc
RI complexes into lipid rafts (Fig. 7C
). This finding supports the concept of heterogeneity of lipid rafts as defined by insolubility in detergents (see above).
Third, aggregation of TEC-21 did not induce co-aggregation of the LynGFP construct as evidenced by confocal micrososcopy (Fig. 9). These data coupled with increased tyrosine phosphorylation of numerous cellular substrates in TEC-21-activated cells and their sensitivity to Src kinase inhibitor PP1 suggest that Lyn kinase need not translocate into TEC-21 patches to initiate cell activation events. This corroborates our previous observations indicating that aggregation of GPI-anchored gp is not followed by an immediate visible co-localization of cytoplasmic Lyn kinase (49). In contrast, aggregated Fc
RI exhibited significant co-localization with Lyn kinase. This co-localization was completely dependent on the anchoring of Lyn kinase into the plasma membrane and only partially dependent on its anchor into the lipid rafts (50).
Finally, aggregated TEC-21 induced a secretory response in cells defective in the expression of FcRI
subunit. These mutants have been previously shown to be defective in the surface expression of Fc
RI
subunit and therefore incapable of binding the IgE (17).
In conclusion, our data indicate that TEC-21 is a novel lipid raft component of RBL-2H3 cells whose aggregation induces early activation events independently of FcRI. The similarity between TEC-21- and Fc
RI-mediated activation events is probably related to the role of lipid rafts as preformed `signalosomes', and their use for initiation of signaling via both aggregated TEC-21 and Fc
RI. Although the function of TEC-21 remains to be elucidated, its unusual tissue specificity, surface expression and association with lipid rafts imply that it exerts a unique function in the communication of cells with extracellular signals. We are currently examining the possibility that expression of TEC-21 in RBL cells is related to their tumor origin.
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Acknowledgments |
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Abbreviations |
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[Ca2+]i concentration of free intracellular Ca2+ |
Fc![]() ![]() |
GFP green fluorescent protein |
gp glycoprotein |
GPI glycosylphosphatidylinositol |
HRP horseradish peroxidase |
PI-PLC phosphatidylinositol-specific phospholipase C |
PNGF peptide N-glycosidase F |
PP1 4-amino-5-(4-methylphenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine |
RBL rat basophilic leukemia |
SFV Semliki Forest virus |
uPAR urokinase plasminogen activator receptor |
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Notes |
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accepted 30 October 2001.
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References |
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