1 Departments of Obstetrics and Gynaecology and 2 Haematology, University College Hospital, London WC1E 6AU, and 3 Division of Physiology, Pharmacology and Toxicology, School of Biological Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK
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Abstract |
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Key words: chemotherapy/Comet assay/DNA damage/fertility/spermatozoa
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Introduction |
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The recent availability of micromanipulation techniques using a single spermatozoon [intracytoplasmic sperm injection (ICSI)] has improved the prospects of child-bearing in patients with severe oligo-asthenozoospermia (Pisarska et al., 1999). Sperm selection techniques do not allow assessment of the genetic integrity of the spermatozoon and it is clear that mutations in the paternal genome can be passed on to the children (Martin, 1996
; Robbins et al., 1997
). Current practice to preserve fertility for patients undergoing genotoxic chemotherapy is to cryopreserve spermatozoa with a view to using them later for assisted reproduction treatment (Tournaye et al., 1993
; Lass et al., 1999
; Pfeifer and Coutifaris, 1999
).
Although it is well known that chemotherapy can adversely effect sperm production, the genetic consequences arising after fertilization with sperm from a treated man are less clear. Aneuploidy of the X and Y chromosomes has been reported in the children of men treated for cancer (Robbins et al., 1997; Monteil et al., 1997
). The integrity of the DNA in the male genome carried by the spermatozoa is crucial for genetic health, and changes can lead to alterations in the germ line which result in the inheritance of lethal and non-lethal conditions (Perreault, 1998
). A major limitation of the current assessment of male fertility is that the genetic integrity of the spermatozoa cannot be easily determined. This is particularly important in view of the known effects of chemotherapy in animal models and the view that normal human ejaculates carry a significant proportion of damaged spermatozoa (Sakkas et al., 1999
)
We have taken the opportunity to see if DNA damage in spermatozoa can be detected in the semen of a patient treated with a chemotherapeutic regime effective for chronic lymphocytic leukaemia (CLL). The patient received FLU, which has not been previously reported to be gonadotoxic and would allow semen collection uncomplicated by reductions in sperm numbers. This case study allowed us to test the hypothesis that FLU causes testicular and sperm DNA damage.
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Materials and methods |
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Collection of samples
Venous blood (20 ml, in EDTA) and semen samples were collected throughout the study period. Plasma was stored at 40°C until assayed. Semen samples were cryopreserved at 70°C and then analysed by the Comet assay as a single batch (see below).
Assessment of testicular function
Testicular assessments included volume by orchidometry and ultrasound. Testicular function was assessed by LH, FSH (Walton et al., 1998) and testosterone immunoassays (Nichols Institute, Newport, Saffron Walden, Essex, UK). Semen parameters were measured using routine in-house methods following the WHO protocols (World Health Organization, 1987
)
Assessment of sperm DNA damage by Comet assay
The comet assay is a widely used technique for measurement of DNA strand breaks in a wide variety of cell types (Olive, 1999). More recently this technique has been used to investigate DNA damage in spermatozoa (Aitken et al., 1998
; Singh and Stephens, 1998
; Hughes et al., 1999
) and we have developed the neutral method for double-strand DNA breaks in human spermatozoa (Haines et al., 1998b
). Sperm cells were cast into miniature agarose gels on microscope slides and lysed in situ to remove DNA associated proteins. The microgels then underwent electrophoresis, which allowed damaged DNA to migrate from the nucleus towards the anode, which could be visualized using fluorescent DNA probes. The length of the tail of DNA extending from the nucleus and the moment (a product of the migration and the percentage of DNA in this tail) were measured by image analysis in >100 sperm cells per time-point, using a fluorescence microscope and Comet Assay II software (Perceptive Instruments, Steeple Bumpstead, Haverhill, Suffolk, UK).
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Results |
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The Comet assay was used as a measure of DNA damage. Spermatozoa assayed from the treated man at the time-points (i)(iv) gave Comet tail lengths (µm, mean ± SEM) of 54.0 ± 1.5, 50.1 ± 0.6, 67.6 ± 0.5, and 54.0 ± 1.4 respectively. The comet tail moments were 10.0 ± 1.67, 6.7 ± 0.2, 14.8 ± 0.2, and 7.5 ± 0.1 units (n = 100200 spermatozoa, mean ± SEM). The values at time-point (iii) were clearly elevated. This was in contrast to values obtained for the control subject which ranged randomly with time from 54.265.7 µm tail length and 4.96.3 units tail moment. The mean ± SD Comet data from the eight 3-monthly samples was 5.64 ± 0.56 units tail moment and 58.70 ± 4.46 µm tail length. Further information on the effect of cytotoxic treatment was obtained by inspection of the distribution of DNA damage in single spermatozoa from the ejaculate (Figure 1). The Comet tail length distribution from the eight samples from the control subject was very similar to the pre-treatment pattern from the patient. The distributions from the first and last sample taken from the control man are given in Figure 2
. In the control sample and the pre-treatment sample from the patient it can be seen that DNA damage was detected in all spermatozoa and that there was a wide distribution in the level of DNA damage. In control samples, there was a clear population with low amounts of DNA damage (i.e. shorter tail lengths). The frequency distribution pattern of sperm Comet lengths was monophasic and skewed to the left with a frequency peak about 4550 µm. The number of spermatozoa with higher amounts of DNA damage was much greater after 7 months of FLU chemotherapy (Figure 1
, panel iii) and the distribution was skewed to the right with a peak at 75 µm. The population of spermatozoa containing lower amounts of DNA damage had almost disappeared. Eleven months after the withdrawal of FLU and GnRH therapy, there was a marked reversal towards the control pattern.
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Discussion |
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In animal models, cytotoxic treatment of the male is associated with genetic damage in the spermatozoa, which is transferred to the offspring. These mutations vary in severity, being expressed diversely as embryo lethality, developmental abnormalities, or changes in coat colour (Perreault, 1998). In contrast, genetic effects of cytotoxic drugs or radiation upon the human male genome, which may be transmitted to the children, have not been conclusively demonstrated, and their occurrence is still controversial (Meistrich, 1993
).
Surprisingly, DNA damage in spermatozoa obtained from normal human ejaculate has been detected by several methods (Sakkas et al., 1999). A major drawback of many of these methods is that spermatozoa and somatic cells in the human ejaculate are heterogeneous, and therefore single-cell analysis would be more informative. The terminal deoxynucleotidyl transferase-mediated dUDP nick-end labelling (TUNEL) technique labels DNA breaks in spermatozoa, and most probably identifies apoptotic DNA strand breaks carried over from meiosis. The numbers of TUNEL-labelled spermatozoa are related to infertility (Sun et al., 1997
).
Recently, Comet analysis of single cells has been introduced and is extensively used to measure single- and double-strand DNA breaks in somatic cells (Olive, 1999). This technique has been applied to spermatozoa, and the method described in the present study allows the detection of double-strand breaks in single spermatozoa (Haines et al., 1998b
). We have confirmed the observations of others that DNA damage can be detected by Comet in spermatozoa; our data also show that spermatozoa in the normal ejaculate carry a range of DNA damage. In the patient sample we cannot discount the possibility that this may in part be due to the previous treatment with chlorambucil (Clark et al., 1995
). However, low numbers of spermatozoa containing high amounts of DNA damage were also present in a similar proportion of spermatozoa from the untreated control man. The origin of this damage is not clear and may arise during spermatogenesis or in the male reproductive tract during transport from the testis to the ejaculate (Aitken, 1999
; Sakkas et al., 1999
).
In the present study we have shown for the first time by direct measurement that DNA damage in spermatozoa is substantially increased by cytotoxic treatment. A particular concern is whether the DNA damage has been resolved 11 months after the end of treatment. We are not able to conclude from the present data if the DNA damage to spermatozoa arising during chemotherapy is reversible, as there is a wide spectrum of DNA damage in spermatozoa from the normal ejaculate. Recent observations in the mouse, however, show that DNA damage persists in spermatozoa for up to 120 days after a single dose of irradiation (Haines et al., 1998a), and as this is longer than one spermatogenic cycle it may be a permanent effect (Morris et al., 1996
).
Of particular concern to men is the biological significance of fertilization of an oocyte by a spermatozoon carrying high amounts of DNA damage. Spermatozoa carrying significant DNA damage are motile and capable of fertilization (Mikamo et al., 1991; Twigg et al., 1998
; Ahmadi and Ng, 1999
) but it is not known what the effect of DNA damage in spermatozoa has upon embryo development, pregnancy, or the subsequent health of the children. Chromosomal aneuploidies have been reported in the children of men conceived after treatment with chemotherapy, and it would be premature to rule out more subtle effects. In animals, cytotoxic treatment of males results in high frequencies of abnormalities both in utero and postnatally (Perreault, 1998
). Recent in-vitro experiments in the mouse have shown that fertilization by spermatozoa with radiation-induced TUNEL DNA damage results in impaired development of the embryos and reduced numbers of live offspring (Ahmadi and Ng, 1999
).
With the increasing success of multimodal cancer therapy, a significant proportion of men will survive the malignancy. Fertility is therefore a principal concern, and health care providers are increasingly aware of the need to improve the quality of life of cancer patients by maintaining normal reproductive function. In the past, recovery of sperm output has been of major concern and it may remain low or take several years to increase to an acceptable level after discontinuation of cancer therapy (Meistrich, 1998). With the advent of assisted reproduction by ICSI it is now possible to offer a good chance of conception in all men with a low sperm output. Given that the sperm selection techniques are based primarily on motility (Nagy et al., 1998
), there is a significant chance, especially in treated patients, that spermatozoa with high amounts of DNA damage are selected. The consequences of this selection are presently not known; however, as they may theoretically be associated with genetic changes to the child, the technique should be used with caution and the genetic outcome of this technique closely scrutinized.
In conclusion, this case study indicates that FLU induces testicular damage within a month and markedly increases DNA damage carried by spermatozoa by 7 months of treatment. As the numbers of spermatozoa carrying high amounts DNA damage may persist after discontinuation of treatment, the DNA damage may be permanent. This observation is worrying and the possibility of biological effects being transferred to the offspring must be considered and investigated further. Fertility counselling of patients undergoing cytotoxic chemotherapy should take into account these effects and it may be pertinent to reappraise the most appropriate timing of semen collection for fertility treatment.
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Notes |
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References |
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Submitted on May 13, 1999; accepted on December 16, 1999.