Istanbul Memorial Hospital, Reproductive Endocrinology and ART Unit, Piyalepasa Cad.-Okmeydani, Istanbul, Turkey
Dear Sir,
We have read the paper by Viville et al. with great interest (Viville et al.2000). As mentioned by the authors, we have experienced poor fertilization and pregnancy rates with inevitable use of megalo-pinhead spermatozoa in intracytoplasmic sperm injection (ICSI) (Kahraman et al.1999
). We have recently performed multicolour fluorescence in-situ hybridization (FISH) analyses with five DNA probes for simultaneous detection of chromosomes X, Y, 13, 18 and 21 in embryos developed from megalo-pinhead spermatozoa. Out of 35 embryos analysed from seven cases, 21 (60%) revealed chromosomal abnormalities (Figure 1
). The majority of the chromosomally-abnormal embryos had aneuploidy (62%). Other abnormalities were triploidy (19%), haploidy (9.5%) and complex aneuploidy (9.5%). The high rate of aneuploidy in embryos developed from this particular form of spermatozoa was striking.
|
Notes
1 To whom correspondence should be addressed. E-mail: kyakin{at}hotmail.com
References
Int Veld, P.A., Broekmans, F.J., de France, H.F. et al. (1997) Intracytoplasmic sperm injection (ICSI) and chromosomally abnormal spermatozoa. Hum. Reprod., 12, 752754.[Abstract]
Kahraman, S., Akarsu, C., Cengiz, G. et al. (1999) Fertility of ejaculated and testicular megalohead spermatozoa with intracytoplasmic sperm injection. Hum. Reprod., 14, 726730.
Kahraman, S., Bahçe, M., Samli H. et al. (2000) Healthy births and on-going pregnancies obtained by preimplantation genetic diagnosis in patients with advanced maternal age and recurrent implantation failure. Hum. Reprod., 15, 20032007.
Kishikawa, H., Tateno, H. and Yanagimachi, R. (1999) Chromosome analysis of BALB/c mouse spermatozoa with normal and abnormal head morphology. Biol. Reprod., 61, 809812.
Lee, J.D., Kamiguchi, Y. and Yanagimachi, R. (1996) Analysis of chromosome constitution of human spermatozoa with normal and aberrant head morphologies after injection into mouse oocytes. Hum. Reprod., 11, 19421946.[Abstract]
Nagy, Z.P., Liu, J., Joris, H. et al. (1995) The result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters. Hum. Reprod., 10, 11231129.[Abstract]
Vegetti, W., Van Assche, E., Frias, A. et al. (2000) Correlation between sperm parameters and sperm aneuploidy rates investigated by fluorescence in-situ hybridization in infertile men. Hum. Reprod., 15, 351365.
Viville, S., Mollard, R., Bach, M.L. et al. (2000) Do morphological anomalies reflect chromosomal aneuploidies? Hum. Reprod., 15, 25632566.