1 Department of Obstetrics and Gynecology, New York University Medical Center, 660 First Avenue, Fifth Floor, New York, NY 10016, USA and 2 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China
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Abstract |
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Key words: cytogenetic analysis/GV transfer/oocyte maturation
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Introduction |
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In a preliminary study with human oocytes, we demonstrated that a GV can undergo GVBD and a normal first meiotic division even when removed and transferred by electrofusion into the enucleated cytoplast of another GV-stage oocyte (Zhang et al., 1999). However, only small numbers of oocytes were available for this work and low success rates were associated with the GV micromanipulation and electrofusion steps. As a result, study of chromosome number and microtubule configuration in the reconstructed oocytes was very limited. A readily available animal model would help to improve our micromanipulation techniques and to evaluate GV maturation in different cytoplasmic environments. A mouse model has been described by others (Kono et al., 1996
; Takeuchi et al., 1999
).
The aims of this study are to establish a procedure for GV removal and transfer in mouse oocytes, to evaluate the meiotic behaviour of the GV following transfer and to assess chromosome number and meiotic spindle configuration during and following these final stages of nuclear maturation.
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Materials and methods |
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Before and during GV transfer all the immature oocytes were incubated in modified HTF medium supplemented with 10% fetal calf serum (FCS, HyClone, Logan, UT, USA) and 50 µg/ml 3-isobutyl-1-methylxanthine (IBMX, Sigma). This inhibitor of cyclic nucleotide phosphodiesterase prevents GVBD. Control GV oocytes were incubated with IBMX but not were micromanipulated prior to in-vitro maturation.
Cytoplast preparation from enucleated GV oocytes, MII oocytes and zygotes
Oocytes and zygotes were exposed to modified HTF medium supplemented with 10% FCS and 7.5 µg/ml cytochalasin B (Sigma) for 30 min at room temperature prior to enucleation. Following lancing of the zona pellucida with a sharp-tipped pipette, GV and pronuclei were removed from immature oocytes and zygotes respectively, using a bevelled glass pipette (ID: 2025 µm; Figure 1A, B, E and F). Removal of oocyte nuclei at metaphase II was achieved under UV light following staining with Hoechst 33342 (Tsunoda et al., 1988
; Figure 1C, D
). These procedures were performed using micro-manipulators attached to an inverted microscope equipped with a UV light source.
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After electrofusion the complexes were washed three times in modified HTF medium and then placed in HTF medium supplemented with 10% FCS for in-vitro maturation. The GV karyoplast was routinely incorporated into the cytoplast within 40 min. The oocytes were repeatedly monitored during a 24 h incubation for evidence of maturational changes.
Cytogenetic analysis
Oocytes extruding a first polar body within the 24 h incubation period were considered to be mature and were fixed for cytogenetic analysis as described (Tarkowski, 1966). Briefly, each oocyte was transferred into a 1% hypotonic trisodium citrate solution for 15 min before fixation with methanol:acetic acid (3:1) on a clean glass slide. The chromosome spreads were then air-dried and stained with 5 µg/ml Hoechst 33342 in phosphate buffer solution. The number and the structure of the chromosomes were determined under fluorescence microscopy.
Immunocytochemistry
Additional reconstructed oocytes with and without extruded first polar bodies were selected for immunocytochemistry of the meiotic spindle, which was performed using prior published methodology (Liu and Chen, 1994). After removing the zona pellucida by a brief treatment with 1% pronase (Sigma) in modified HTF, each oocyte was fixed in 3.7% formaldehyde (Sigma) in 0.1 mol/l PBS (pH 7.4) for 40 min, and then permeabilized in PBS buffer containing 0.25% Triton X-100 (Sigma) for 25 min at room temperature. Free aldehydes were reduced by a 20 min rinse in 0.1 mol/l PBS containing 150 mmol/l glycine (Sigma). Microtubules were localized with a mouse monoclonal antibody to ß-tubulin (Sigma). The primary antibody was detected using a fluorescence labelled goat anti-mouse IgG (Sigma). Each antibody was applied overnight at 4°C; oocytes were rinsed with PBS containing 0.25% bovine serum albumin (BSA, Fraction V; Sigma) between antibody applications. Chromosomes were simultaneously detected with Hoechst 33342 (5 µg/ml in 0.1 mol/l PBS). The stained oocytes were mounted whole on glass slides and examined using light phase and fluorescence microscopy.
Data analysis
Data were analysed using the 2 test with significance determined at P < 0.05.
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Results |
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Discussion |
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We also tested if a GV can resume meiosis and mature when transferred into the cytoplasm of an anuclear zygote. It has been suggested that zygote cytoplast status may be similar to that of the GV oocyte (Campbell et al., 1996). Zygotes have exited from metaphase arrest after fertilization and display a low level of maturation, or meiosis, promoting factor (MPF) activity. MPF is an important factor for meiosis resumption, including the induction of GVBD, chromosome condensation and final nuclear maturation (meiotic divisions). MPF is composed of p34cdc2 and cyclin B and the phosphorylation state of p34cdc2 in zygotes is similar to that reported in the ooplasm during the GV stage (Choi et al., 1991
). Moreover, cytoplasmic factor(s) in the zygote can decondense the MII oocyte chromosome and enclose it in a nuclear envelope, thereby enabling the cell to enter into a state of interphase and began new cell cycle (Balakier and Masui, 1986
). Nonetheless, our results indicate that a GV transferred into an enucleated zygote cytoplast rarely undergoes GVBD and cannot extrude a polar body.
Finally, we tested if a GV resumes meiosis when transferred into the cytoplasm of an enucleated MII stage oocyte. Our results indicate that ~20% of such reconstructed oocytes undergo GVBD but none extrudes a first polar body. This result, also observed by others (Takeuchi et al., 1999), is surprising considering that MPF activity is relatively high in oocytes at metaphase II (Hashimoto and Kishimoto, 1988
). Moreover, during cloning studies (Wakayama et al., 1998
), it was noted that when a somatic cell nucleus (G0 or G1 cell cycle stage) is transferred into an enucleated MII oocyte, polar body extrusion normally occurs following activation. Mitosis and cell division only ensued when the reconstructed clone was suppressed with cytochalasin B treatment. There are two potential explanations for the different outcomes observed in this study and in that of Wakayama. The first is that chromosome number is an important determinant for the first stage of meiosis to progress. The oocyte GV (G2 stage) contains 4n chromosomes, whereas only 2n chromosome are present in somatic cells, especially those in cell cycle stage G0. The more likely explanation, however, involves the different procedures used to insert a cell nucleus into enucleated metaphase II eggs. Wakayama and his co-workers injected somatic cell nuclei directly, whereas we had to employ electrofusion to transfer a GV into an oocyte or zygote cytoplast due to the large size of the GV. Significantly, the minimal parameters for this electrofusion procedure are nonetheless sufficient to activate human oocytes (Zhang et al., 1999
). Thus, it is likely that the metaphase II cytoplast was activated during the transfer resulting in inappropriate cytoplasmic conditions for the progression of meiosis. Analysis of the effects of electrofusion on ooplasmic levels of MPF and other factors will be the topic of a future study.
In conjunction with in-vitro maturation, several advantages can be gained from the development of oocytes reconstructed by GV nucleus transfer. First, these reconstructed oocytes may be useful models to elucidate the cytoplasmic and nuclear interrelationships underlying the onset and progression of meiosis. Secondly, because we can exchange GVs between oocytes of different quality or age, GV transfer may be used to determine the role of the ooplasm in chromosome disjunction and whether its actions become compromised in oocytes which show genetic or developmental anomalies in maturation, e.g. the increased incidence of aneuploidy in oocytes of women of advanced maternal age (Eichenlaub-Ritter, 1996; Angell, 1997
).
Further studies are necessary to determine whether normal offspring are produced using reconstructed oocytes. Even though reconstructed oocytes appear morphologically normal, their ability to fertilize and develop normally through early embryonic life must be established. Studies in mice have reported the birth of live offspring following the multiple nuclear transfers at the GV and MII stages (Kono et al., 1996).
As we have stated previously (Zhang et al., 1999), GV transfer could potentially become a new approach in treatment of human infertility due to advanced maternal age. Despite the fact that diagnostic, clinical and laboratory techniques have improved significantly since the introduction of IVF, donated oocytes remain the only solution for achieving pregnancy in women who are unable to produce normal quality oocytes. With the nuclear transfer technique it may be possible to construct normal quality oocytes for these women by transferring the GVs from their oocytes into cytoplasts generated from oocytes donated by young healthy women.
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Notes |
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References |
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Submitted on April 27, 1999; accepted on June 11, 1999.