1 Division of Endocrinology, Department of Internal Medicine, School of Medicine, University of Chile, 2 Institute of Maternal and Child Research, University of Chile, Santiago, 3 Laboratory of Animal Physiology and Endocrinology, School of Veterinary Medicine, University of Concepción, Chillán, Chile, 4 Division of Gynecological Endocrinology and Reproductive Medicine, Department of Obstetrics & Gynecology, University of Erlangen, Germany
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Abstract |
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Key words: Kallmann's syndrome/leptin/LH/pulsatility
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Introduction |
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A synchronicity of LH and leptin pulses in the mid-to-late follicular phase of the menstrual cycle of healthy women has been demonstrated (Licinio et al., 1998), suggesting that leptin may regulate the minute-to-minute oscillations in plasma concentrations of LH. Recently we extended these observations to patients with polycystic ovarian syndrome (PCOS), demonstrating that circulating leptin and LH are synchronized in patients with PCOS, but weaker (only 20 of 39 pulses) and with a phase-shift greater than in normal women (Sir-Petermann et al., 1999
).
There are several ways to explain the phenomenon of coupling of LH and leptin release. The first is that LH regulates leptin secretion. The second is that leptin regulates LH secretion and the third is that both hormones are driven by a common oscillator whose nature and location are not currently known. To further examine the relationship between LH and leptin, we studied the episodic fluctuations of circulating LH and leptin in patients with hypogonadotrophic hypogonadism and anosmia (Kallmann's syndrome, KS) before and during pulsatile GnRH administration, and compared them with those observed in regularly menstruating women. KS offers a unique opportunity to study LHleptin interactions in a state of natural inactivity of the gonadal axis, caused by the absence of hypothalamic GnRH secretion.
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Materials and methods |
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In addition, 10 normally cycling women of similar age, divided into two groups according to their BMI, acted as the control groups. None of these women had taken oral contraceptives or other medication for at least 6 months before starting the study. Prior to the study, informed consent was obtained from all subjects. This study was approved by the local ethical committee. The clinical characteristics of the two KS patients and the two control groups are presented in Table I.
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KS patients were studied before the initiation of therapy and during the seventh day of pulsatile GnRH administration. Control women were studied in the early follicular phase of the menstrual cycle (day 37).
For the study of episodic hormone secretion, blood samples were collected at 10 min intervals for 6 h, using a sampling device that allowed the continuous withdrawal of blood through a heparinized catheter (Sir-Petermann et al., 1995). LH and leptin were determined in all samples. Oestradiol was determined in samples 1, 19 and 37.
GnRH administration
In both KS patients a positive withdrawal bleed occurred after the administration of oral contraceptives. Beginning day 2 after withdrawal bleed, GnRH was administered i.v. with a battery-powered time auto-infusion pump (Zyklomat; Ferring, Kiel, Germany) at a rate of 5 µg every 90 min as described previously (Leyendecker et al., 1980). The solution was infused for 28 days through a heparinized cannula inserted into the forearm vein on an ambulatory out-patient basis. No complications were observed.
Hormone assays
Serum LH and oestradiol were determined by electrochemiluminescense (Boehringer Mannheim, Mannheim, Germany; range: 0.1200 IU/l for LH and 104000 pg/ml for oestradiol), total leptin was measured by radioimmunoassay (Linco-Research Inc., St Louis, MO, USA). The intra- and inter-assay coefficients of variation respectively were 1.1 and 2.1% for LH; 2.5 and 3.6% for leptin and 2.7 and 8% for oestradiol.
Pulse analysis and statistical evaluation
For pulse analysis, the computerized version of the cluster pulse algorithm, (Veldhuis and Johnson, 1996), was used. We selected a cluster configuration of 1x2 (one sample for the test peak and two for the test nadir), and a t-value of 2.1/2.1 to constrain the likelihood of false positive pulse determination to <5%. The following mean properties of pulsatile hormone concentrations were analysed: pulse frequency (number of significant peaks/6 h), pulse amplitude, pulse height and pulse area.
The mean value of each hormone parameter in each subject was calculated (control subjects and KS patients). Because this was a clinical study, where one single subject was compared with reference values obtained from a group, the 95% prediction limits were determined (Whitmore, 1986) to establish whether or not a given new value (mean value of the respective KS patient) was within the range observed in the control group (data obtained from mean values of five control subjects). Results are expressed as means and ranges.
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Results |
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During GnRH administration (seventh day), pulsatile LH activity was induced in both KS patients. The characteristics of the leptin pulsatile pattern were not modified.
Table II shows the serum concentration of LH and LH pulse characteristics in KS patients and the control groups. Before treatment, mean LH concentrations were lower (outside the prediction limits) in KS patients compared to the controls. During GnRH administration, both patients exhibited a LH pulsatile pattern with pulse characteristics similar to those of the respective control group. Serum leptin concentrations and leptin pulse characteristics are presented in Table III
. Basal leptin concentrations and leptin pulse characteristics did not differ between KS patients and the respective control groups. During GnRH administration, serum leptin concentrations and leptin pulse characteristics were not modified.
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Discussion |
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In healthy women and in patients with PCOS, a synchronicity of LH and leptin pulses has been observed, suggesting a coupling of LH and leptin release (Licinio et al., 1998; Sir-Petermann et al., 1999
). The stimulatory effect of leptin on the neuroendocrinereproductive axis has been established in some species, including rat (Cagampang et al., 1990
), monkey (Finn et al., 1998
; Nagatani et al., 1998
) and human (Laughlin et al., 1997
, 1998
). However, on the contrary, it has not been demonstrated whether LH regulates leptin secretion.
The pattern of pituitary hormone secretion may be viewed as being the consequence of the activity of a central oscillator modulated by internal or external factors, and could account for the modulatory effect of leptin on GnRHLH secretion. In this respect, it appears to be more likely that leptin regulates GnRHLH secretion than vice versa. This assumption is based on a study in girls with precocious puberty treated with GnRH analogues in which leptin pulsatility persists despite the inhibition of the gonadal axis (Palmert et al., 1998), as well as on the observation made in our previous study (Sir-Petermann et al., 1999
), in relation to the dynamics of hormone secretion. Leptin concentrations increased in blood before or concomitantly with but seldom after LH peaks. According to these observations and the data of the present study, it is proposed that circulating leptin might regulate GnRHLH secretion, but GnRHLH secretion is apparently not involved in modulating episodic leptin release, thus suggesting that the episodic leptin release is not driven by the GnRH pulse generator.
The mechanisms that account for episodic leptin release are not completely understood. Ultradian rhythmicities are common characteristics of many systems (Lavie and Kripke, 1981), including the hormonal system (Brandenberger, 1992
). According to this point of view, ultradian leptin release could be seen as part of a general phenomenon, this assumption being based on the fact that in our KS patients the pulse generator of leptin was intact. However, Simon et al. (1998) established recently that 50% of the leptin pulses were preceded by an insulin or glucose pulse. These results suggest that glucose and insulin ultradian oscillations may affect subsequent leptin release, implying that the ultradian leptin pulses may be driven by a peripheral oscillator, probably related to pancreatic activity (Sirek et al., 1985
; Simon et al., 1987
). Taking all these observations into account, we propose that fluctuations in insulin and glucose concentrations drive the ultradian leptin secretion, which in turn is coupled to the LH pulsatile secretion. The real significance of the coupling of these hormones must be elucidated; it probably reflects a general regulatory phenomenon in which the secretion of a regulatory factor precedes or is concomitant with the secretion of the target factor.
In summary, we demonstrated that there are no differences between normal and KS women in the pulsatile characteristics of circulating leptin, and finally that circulating leptin is not modified by exogenous GnRH administration.
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Acknowledgments |
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Notes |
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References |
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Submitted on April 7, 1999; accepted on July 22, 1999.