Reproductive decisions of men with microdeletions of the Y chromosome: the role of genetic counselling

Annemiek W. Nap1, Ron J.T. Van Golde1, Joep H.A.M. Tuerlings2, Paul De Sutter3, Math H.E.C. Pieters4, Jacques C. Giltay5, Peter M.M. Kastrop6, Didi D.M. Braat1 and Jan A.M. Kremer1,7

1 Department of Gynaecology and Obstetrics, 2 Department of Human Genetics, University Hospital Nijmegen, The Netherlands, 3 Department of Obstetrics and Gynaecology, University Hospital Gent, Belgium, 4 Laboratory of In vitro Fertilisation, University Hospital Dijkzigt, Rotterdam, 5 Clinical Genetics Centre, Utrecht and 6 Department of Obstetrics and Gynaecology, University Hospital Utrecht, The Netherlands


    Abstract
 Top
 Abstract
 Introduction
 Materials and methods
 Results
 Discussion
 References
 
Couples dealing with microdeletions of the Y chromosome have to make decisions about their reproductive future. Do they opt for intracytoplasmic sperm injection (ICSI), artificial insemination with donor insemination (AID) or no treatment? We analysed this decision in 28 couples and investigated the role of the counsellor and the counselling process on the final decision of the couple. Ten counsellors from six fertility clinics in The Netherlands and Belgium were interviewed about their genetic counselling of couples dealing with microdeletions. The answers to the questionnaire were converted to 11 dichotomous variables. Of the 1627 tested men in the six centres, 37 (2.3%) had a microdeletion in the AZFc region, a subregion of the AZF region on the Y chromosome important for normal spermatogenesis. The decisions of 28 of them could be analysed. Most couples chose ICSI (79%). The remaining couples chose donor insemination (7%) or refrained from treatment (14%). Several variables, including the counselling procedure, the counsellor and the available treatments in the fertility centre, influenced the decision of the couple. In conclusion, most couples dealing with microdeletions in the AZF region choose ICSI. Several aspects of the process of genetic counselling appear to be related to the final decision.

Key words: assisted reproduction/genetic counselling/ICSI/male infertility/microdeletions


    Introduction
 Top
 Abstract
 Introduction
 Materials and methods
 Results
 Discussion
 References
 
Men with microdeletions of the Y chromosome and their wives have to make decisions about their reproductive future. Do they choose intracytoplasmic sperm injection (ICSI), artificial insemination with donor semen (AID) or no treatment? Little is known about this decision-making and the role of genetic counselling.

Microdeletions in the azoospermia factor (AZF) region of the Y chromosome have been described in oligozoospermic and azoospermic men (Tiepolo et al., 1976; Ma et al., 1992Go; Vogt et al., 1992Go; Reijo et al., 1996Go; Pryor et al., 1997Go; Kremer et al., 1998). Most deletions are found in the AZFc region (Simoni et al., 1998Go). After the introduction of ICSI (Palermo et al., 1992Go), men with microdeletions in the AZF region could father children despite their severe oligozoospermia or azoospermia. Recently, transmission of microdeletions to sons via ICSI has been described (Silber et al., 1998Go). So, presumably men with microdeletions will transmit the deletion, as well as the related fertility problem, to their sons.

Many in-vitro fertilization (IVF) clinics offer their patients with severe oligozoospermia or azoospermia testing for microdeletions in the AZF region. If a deletion is found, a decision has to be made by the couple. Different authors have emphasized the importance of genetic counselling in this process (Mau et al., 1997Go; Pauer et al., 1997Go; Tuerlings et al., 1997Go). As stated before, little is known about the impact of having a microdeletion on further decision-making on reproductive options. In the present study, we analysed this decision-making and investigated the relationship between some aspects of the process of genetic counselling and the final decision.


    Materials and methods
 Top
 Abstract
 Introduction
 Materials and methods
 Results
 Discussion
 References
 
In order to obtain information about the genetic counselling and reproductive decisions taken by couples dealing with microdeletions in the AZF region of the Y chromosome, 14 fertility clinics in The Netherlands and Belgium were contacted and asked to participate in the study.

In the participating clinics, counsellors were interviewed about the genetic counselling of couples dealing with microdeletions of the Y chromosome. A questionnaire containing 30 questions was used, focusing on the information given about Y deletions and the consequences for the offspring, the treatment options available and the counsellors' own opinion about Y deletions. Counselling on ICSI included information about microdeletions on the Y chromosome being the cause of the fertility problem and the risk of transmitting fertility problems to their male offspring. Counselling on AID included the information that there is no increased risk from the use of genetically abnormal spermatozoa. Furthermore, the reproductive decisions of the individual couples were documented. This information was gathered under supervision of a representative of the centre and handled anonymously.

Subsequently, we analysed the relationship between the answers of the counsellor to the questionnaire and the final decision. The answers to the questions were converted to 11 dichotomous variables that were thought to be related to the decisions of the couple (Table IGo). Statistical analysis was performed by {chi}2 tests.


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Table I. Dichotomous variables related to the counselling of men with Y deletions, questions asked and answers given to the counsellors, and the interpretation of the answers (in italics). Reproductive decisions towards: intracytoplasmic sperm injection (ICSI), artificial insemination with donor semen (AID) or no treatment
 

    Results
 Top
 Abstract
 Introduction
 Materials and methods
 Results
 Discussion
 References
 
Study group
Fourteen IVF centres were contacted to participate in the study (until August 1998). One centre did not respond. In two centres, diagnostic tests to detect Y deletions were not offered to the patients. In two centres, the patients were referred to other centres for diagnostic tests. Three centres offered diagnostic research on Y deletions, but no Y deletions were detected. In the remaining six centres (five in The Netherlands and one in Belgium), 1627 men were tested and 37 men appeared to have a microdeletion in the AZFc region of the Y chromosome (2.3%). One couple did not want to be genetically counselled and eight couples had not taken a decision at the time of the study. These nine couples were excluded from the study.

The mean age of the men in the remaining 28 couples was 34.7 years (range 24–48). The mean age of the women was 31.9 years (range 23–43).

Genetic counselling
The 28 couples in the six centres were counselled by 10 different counsellors: four clinical geneticists, three gynaecologists, two andrologists and one embryologist. The counselling was performed during one to three sessions with one or two counsellors.

Couples' reproductive decisions
Twenty-two couples choose ICSI (79%). Of the six couples who did not choose ICSI, two couples opted for AID (7%), and four couples decided to refrain from either form of treatment (14%).

The variables derived from the answers to the questionnaire, and their statistical analysis using {chi}2 are shown in Table IIGo. Seven variables were significantly related to the final decision. Four of these seven variables were significantly related to the choice for ICSI: adequate counselling about Y deletions (P = 0.01), thorough counselling about Y deletions (P = 0.002), thorough counselling about risks of ICSI for the offspring (P = 0.02) and only ICSI available in the department of the counsellor (P = 0.04). Three of the seven variables were significantly related to the choice for the alternatives of ICSI: Y deletions are serious defects according to the counsellor (P = 0.02), only AID available in the department of the counsellor (P = 0.002) and directive counsellor (P = 0.002).


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Table II. The variables related to the counselling of 23 men with Y deletions (Table IGo) and the final decision of the couple
 

    Discussion
 Top
 Abstract
 Introduction
 Materials and methods
 Results
 Discussion
 References
 
Our data show that about 80% of the couples dealing with microdeletions in the AZFc region choose ICSI rather than AID, adoption or no treatment. This high frequency is consistent with the findings of a Dutch study describing reproductive decisions of couples dealing with chromosomal abnormalities (Giltay et al., 1998). The decisions of the couples in this study and in our study are not influenced by financial motives, since the ICSI treatment is almost completely reimbursed in the Netherlands and partially in Belgium.

One may conclude that testing for microdeletions is not useful: with testing most couples choose ICSI, whereas without testing probably most people will also choose ICSI. We do not agree with this conclusion. First of all, a reasonable percentage of the couples decided to refrain from ICSI after testing. Furthermore, little is known about the consequences and risks of ICSI. Therefore we think that we have the obligation to inform the couple about what we do know. We have to inform them in an optimal way and offer all ICSI men with severe oligozoospermia or azoospermia testing for microdeletions in the AZF region. After genetic counselling, the couple can make their own and well-informed choice about their reproduction.

Each counsellor tries to counsel in an objective way and wants to think that the couple makes a free decision. However, this seems not to be the case. Our data show that the decision of the couple to choose ICSI or its alternatives is related to a number of variables of the process of genetic counselling. The clinic of the counsellor is important: if only ICSI is available more couples choose ICSI, and if AID is the only available treatment more couples choose AID. Additionally, the counsellor is important: if he or she has the opinion that microdeletions are serious defects and if he or she counsels in a directive way, more couples refrain from ICSI. Finally, the process of counselling is important: if this process is judged by the counsellor as thorough and adequate, more couples choose ICSI.

We performed a multicentre study to get a large study group. However, the numbers remain too small to perform a multivariate analysis on the data. Because the analysed variables are not independent, care must be taken when interpreting each individual variable. Moreover, we only demonstrated a statistical relationship between the variables and the final decision and not a causal relationship.

Pre-implantation genetic diagnosis (PGD) and sex-selection is an alternative strategy for couples dealing with microdeletions. However, this possibility was not available in the six centres studied. We know that there are clinics in Belgium that offer PGD to couples dealing with microdeletions, but the results have not been published until now.

Only 37 of the 1627 tested men had a microdeletion in the AZFc region (2.3%), which is lower than the frequency in earlier studies (reviewed by Simoni et al., 1998Go). This may be due to the fact that a majority of the tested men had oligozoospermia and not azoospermia, since ICSI with surgically retrieved spermatozoa is not allowed in The Netherlands. Microdeletions are found more frequently in azoospermic men than in oligozoospermic men (Simoni et al., 1998Go). An alternative explanation for the relatively low frequency may be publication bias of positive results in earlier studies.

We conclude that most couples dealing with microdeletions of the Y chromosome choose ICSI and that a minority choose an alternative. Several aspects of the process of genetic counselling are related to the decision-making. Each counsellor should be aware of this phenomenon and should try to counsel in an objective and optimal way, so that the couples can make well-informed choices about their reproductive future.


    Acknowledgments
 
We would like to thank Professor Dr F.H.Comhaire, Professor Dr M.Dhont, Professor Dr F.van der Veen, Dr L.C.P.Govaerts, Dr G.R.Dohle, M.D. and J.C.Dolfing, M.D. for their co-operation in the study.


    Notes
 
7 To whom correspondence should be addressed at: Department of Gynaecology and Obstetrics, University Hospital Nijmegen,PO Box 9101, 6500 HB Nijmegen, The Netherlands Back


    References
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 Abstract
 Introduction
 Materials and methods
 Results
 Discussion
 References
 
Giltay, J.C., Kastrop, P.M.M., Tuerlings, J.H.A.M. et al. (1999) Subfertile men with constitutive chromosome abnormalities do not necessarily refrain from intracytoplasmic sperm injection treatment: a follow-up study on 75 Dutch patients. Hum. Reprod., 14, 318–320.[Abstract/Free Full Text]

Kremer, J.A.M., Tuerlings, J.H.A.M., Meuleman, E.J.H. et al. (1997) Microdeletions of the Y chromosome and intracytoplasmic sperm injection: from gene to clinic. Hum. Reprod., 12, 687–691.[Abstract]

Ma, K., Sharkey, A., Kirsch, S. et al. (1992) Towards the molecular localisation of the AZF locus: mapping of microdeletions in azoospermic men within 14 sub-intervals of interval 6 of the human Y chromosome. Hum. Mol. Genet., 1, 29–33.[Abstract]

Mau, U.A., Backert, I.T., Kaiser, P. et al. (1997) Chromosomal findings in 150 couples referred for genetic counselling prior to intracytoplasmic sperm injection. Hum. Reprod., 12, 930–937.[ISI][Medline]

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Pryor, J.L., Kent-First, M., Muallem, A. et al. (1997) Microdeletions in the Y chromosome of infertile men. N. Engl. J. Med., 336, 634–639.

Reijo, R., Alagappan, R.K., Patrizio, P. et al. (1996) Severe oligozoospermia resulting from deletions of azoospermia factor gene Y chromosome. Lancet, 347, 1290–1293.[ISI][Medline]

Silber, S.J., Brown, L.G., Alagappan, R. et al. (1998) Pregnancies and children from ICSI using sperm of Y-deleted azoospermic and severely oligospermic men: transmission of the Y deletion to male offspring (Abstract). IFFS/ASRM Annual Meeting, San Francisco. Fertil. Steril. Suppl., O-038, S15.

Simoni, M., Kamischke, A. and Nieschlag E. (1998) Current status of the molecular diagnosis of Y chromosomal microdeletions in the work-up of male infertility. Initiative for international quality control. Hum. Reprod., 13, 1764–1768.[Free Full Text]

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Submitted on February 15, 1999; accepted on May 10, 1999.