Department of Obstetrics and Gynaecology, Göteborg University, SU/Sahlgrenska, 413 45 Gothenburg, Sweden
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Abstract |
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Key words: human oocyte/ICSI/MII spindle/polar body
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Introduction |
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The objective of this study was to document the actual spatial relationship between the first PB and the MII spindle in in-vivo matured human MII oocytes (freshly aspirated) and in oocytes matured in vitro. It has been speculated that an increased distance between the PB and the MII spindle might be brought about by the denudation procedure (Hewitson et al., 1999). By studying immature oocytes which were denuded before the extrusion of the first PB, we hoped to shed some light on why a difference, if any, was found between the first PB and the second MII spindle in the in-vivo matured oocytes.
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Materials and methods |
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Immunostaining for tubulin
In order to assess the spatial relationship between the first PB and the second MII spindle, a method employing double fluorescence staining was used. The oocytes were fixed for 20 min at 37°C in a microtubule stabilizing buffer (0.1 PIPES, pH 6.9, 5 mmol/l MgCl2.6H2O, 2.5 mmol/l EGTA containing 2.0% formaldehyde, 0.5% Triton X-100, 1 µmol/l taxol), then washed three times in a blocking solution of phosphate-buffered saline (PBS) with 2% bovine serum albumin (BSA), 2% powdered milk, 2% normal goat serum, 0.1 mmol/l glycine and 0.01% Triton X-100. The oocytes were attached to poly-L-lysine coated glass slides. For microtubular visualization, the oocytes were incubated in anti- tubulin monoclonal antibody (Amersham International, Amersham, Buckinghamshire, UK) for 1 h at 37°C, at 1:1000 in PBS containing 0.1% BSA and 0.02% sodium azide (PBS + sodium azide). The slides were washed for 1 h at room temperature in blocking solution and further incubated in a 1:100 solution of Cy3 conjugated goat anti-mouse immunoglobin G (IgG; Amersham International) for 1 h at room temperature. After this step, the oocytes were washed three times in PBS + sodium azide and chromosomes identified by counterstaining with 4',6'-diamidino-2-phenylindole (DAPI) (500 ng/ml; Sigma, St Louis, MO, USA) diluted in mounting medium (Vectashield; Vector Laboratories, Inc., Burlingame, CA, USA) for 10 min at room temperature. The oocytes were examined using a Nikon epifluorescence microscope equipped with appropriate filters. Images were transferred via a video camera using a data imaging program (Applied Imaging, Scotswood Road, Newcastle upon Tyne, UK) and stored in a computer. Although a simpler method of staining the oocytes would have sufficed, data for this publication were drawn from another parallel study in which oocyte fixation and staining of the spindle apparatus for visualization was necessary.
Although the oocytes were attached to the glass with their PB perpendicular to the observer, not all the spindles had a cortical position, i.e. when observed, they appeared to be positioned somewhere inside the oocyte. Since the MII spindle has been shown to be attached to the inner cell surface (Johnson et al., 1975; Longo and Chen, 1985
; Szöllösi et al., 1986
), it should be possible to measure the angle between the PB and the MII spindle in a two-dimensional view. Therefore, the oocytes which were observed with their MII spindles `inside' the ooplasm were `rotated' relative to the cortex (Figure 1
). This was achieved by drawing an imaginary line through the PB and the centre of the oocytes and then moving the MII spindles by rotation of the oocytes perpendicularly around this axis until the spindles were situated close to the cortex. By this procedure, all the angle measurements were made with the MII spindle close to the cortex of the oocyte.
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Results |
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Discussion |
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In order to build up the MII spindle, the oocyte has to complete its first meiosis, extrude the first PB, enter the second meiosis and arrest in the second metaphase. This means that the microtubulins that make up the first metaphase spindle have to break down and a new spindle has to be formed. It has been suggested that these events are organized by so-called microtubular organizing centres (MTOC) (Battaglia et al., 1996). These MTOC seem to govern the microtubular organization until the appearance of the sperm aster after fertilization and are found mainly in the cortical area of the oocyte during both MI and MII. It is not known whether the MTOC remain stationary during both MI and MII and it is possible that after completion of the first meiosis, the MII spindle can be built at a new location. Another explanation for how the distance between the first PB and the MII spindle arises might simply be the dynamic movements of the oolemma during and after the first PB extrusion, which has been documented by using time-lapse video recording (own unpublished data). The underlying process might possibly involve the microfilaments in the cortical area of the oocyte.
The fact that the PB of a denuded MII oocyte is not necessarily positioned above or very near the second MII spindle might be cause for concern when performing ICSI. Possible consequences of inserting the ICSI needle into or close to the MII spindle may include total or partial disruption of the spindle or displacement of the spindle from the oolemma. Total disruption of the spindle would eventually lead to cell death and thus higher damage and lower fertilization rates. Partial disruption might result in perturbation of chromosomal segregation and subsequent aneuploidy, as meiosis in the mammalian female does not seem to have rigorous spindle check-points, thus making the gamete prone to errors during meiosis (Fulka Jr et al., 1997; LeMaire-Adkins et al., 1997
). It has been shown that children born after ICSI run a slightly higher risk of chromosomal aneuploidy mainly involving the sex chromosomes (Tournaye et al., 1995
). However, it seems unlikely that only the sex chromosomes would be affected by a possible negative effect of the injection method. A higher prevalence of Klinefelter syndrome and translocations in the population of infertile men needing ICSI seems a more likely explanation (Chandley and Hargreave, 1996
).
Although the fertilization rate after ICSI is fairly good, reaching 6070% in most clinics, this leaves us with the fact that a significant proportion (3040%) of the oocytes are potentially wasted. It has been reported (Flaherty et al., 1995) that in an ICSI programme a majority of the unfertilized MII oocytes contained a swollen sperm head. They also found that in 4% of the unfertilized oocytes the swollen sperm heads were located among the metaphase chromosomes and concluded that the first PB must have moved from the spindle region at the time of injection as they had taken great care in aligning the first PB at the 12 o'clock position before injecting at the 3 or 9 o'clock position. Although a 100% fertilization rate will presumably never be accomplished, the fertilization and survival rates after ICSI might be further increased by altering the position of the injection to the hemisphere opposite to that of the PB.
Recent data, based on hamster (Silva et al., 1999) and rhesus monkey oocytes (Hewitson et al., 1999
), are in accordance with our observation on human oocytes that the PB does not always reside close to the spindle. The clinical validity of the hamster experiments for humans is limited, however, since there does not seem to be any regularity as to where the MII spindle is positioned in relation to the first PB. It has been shown (Hewitson et al., 1999
) that the MII spindle could be displaced up to 68° from the PB in in-vivo matured rhesus monkey oocytes, with a mean difference of 19.8 ± 23.3° (n = 19), which is lower than our findings. Furthermore, they found that in human oocytes matured in vitro (n = 3) and in IVF failures (n = 5) the mean displacement between the PB and the spindle was 12 and 11° respectively. When examining denuded human oocytes intended for ICSI, they found that the displacement had increased to an average of 56 ± 27.5° (n = 8), which is much higher than we found. Although based on a low number of observations, these results do correlate to our findings. In the mouse oocyte, a time-dependent displacement of the MII chromosomes in relation to the first PB has been documented (Kono et al., 1991
). Interestingly, they found that 2 h after the PB extrusion only 10% of the chromosomes were still located directly under the PB and the remaining chromosomes distributed over the whole oocyte, which was not found in our study of the human oocyte.
Recently, some interest has been shown in the polarity of the oocyte/zygote and the possible advantages of this when choosing embryos for transfer (Edwards and Beard, 1997; Fulka Jr et al., 1998
. Garello et al., 1999
). These studies base their morphological evaluation of polarity partly on the position of the polar bodies and the pronuclei. In the study by Garello et al. the placement of a spermatozoon in a fixed plane relative to the first polar body (ICSI) did not result in an altered pronuclear/polar body orientation relative to IVF (Garello et al., 1999
). However, these IVF zygotes were found to have a higher degree of eccentric pronuclei and higher incidence of irregular cleavage or cleavage failure. If the first PB is indeed an important landmark of zygotic polarity, the denudation method used in relation to the ICSI method and the subsequent displacement of the PB might introduce an error, making the validity of this zygote screening questionable.
It is important to point out that in 93% of the oocytes in our study the MII spindle was actually located in the same hemisphere as the PB (the animal pole). Therefore, it might be advantageous when performing ICSI to inject the spermatozoon on the vegetal side of the midline between the two hemispheres of the oocyte to avoid damage to the MII spindle (Figure 2). Furthermore, the opening of the ICSI needle should be pointed towards the animal pole so that the spermatozoon is ejected in the direction of the MII spindle, shown by other investigators to be of benefit for embryonic development (Blake et al., 2000
) and pregnancy rates (Van der Westerlaken et al., 1999
).
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Acknowledgments |
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Notes |
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References |
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Submitted on October 18, 1999; accepted on February 28, 2000.