Reproductive Medicine, BioScience Centre, ICFL, Times Square, Newcastle upon Tyne NE1 4EP, UK
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Abstract |
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Key words: blastocyst formation/early cleavage/first cell cycle/human embryos
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Introduction |
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A number of strategies, ranging from pronucleate stage selection to blastocyst transfer, have been devised to help improve the prediction of embryo viability. Studies in which pregnancy and implantation rates were compared following transfer of blastocyst or cleavage stage embryos have produced conflicting results. While some studies have reported that transfer of blastocysts results in higher implantation rates than transfer of cleavage stage embryos (Gardner et al., 1998; Marek et al., 1999
; Schoolcraft et al., 1999
; Milki et al., 2000
), other studies have found no difference (Coskun et al., 2000
; Huisman et al., 2000
). However, the practice of blastocyst transfer is not in widespread use, partly because of a general lack of experience in prolonged embryo culture, as well as anxieties about those patients whose embryos arrest before blastocyst formation (Van Blerkom, 1997
). Furthermore, an increased incidence of monozygotic twinning after blastocyst transfer has been reported (Behr et al., 2000
; da Costa et al., 2001).
An alternative strategy, which has evolved in situations where culture beyond the zygote stage is not compatible with religious beliefs or legal requirements, is to select embryos on the basis of pronucleate stage morphology. The evidence indicates that a grading system based on morphological characteristics, including the extent of pronuclear apposition and nucleolar alignment, is predictive of implantation potential (Scott and Smith, 1998; Tesarik and Greco, 1999
; Ludwig et al., 2000
) and blastocyst formation (Scott et al., 2000
). Consistent with this, a recent study, encompassing experimental evidence and mathematical modelling, supports the idea that the viability of human embryos is already determined at the 1-cell stage (Hardy et al., 2001
).
The 1-cell stage of development represents the return to mitotic division following completion of maternal meiosis. In the first cell cycle, S phase, in which the chromosomes replicate, and M phase, in which the replicated chromosomes segregate, are separated by two gap phases G1 and G2. Entry into G1 is marked by the appearance of pronuclei, which persist until the transition from G2 into M phase. Observations of human zygotes indicate variability in the timing of all cell cycle transitions following sperm entry (Balakier et al., 1993; Capmany et al., 1996
; Payne et al, 1997
; Nagy et al., 1998
). The culmination of this is that the onset of first cleavage (exit from M phase) is observed over a time-span of >8 h (between 22 and >30 h after sperm entry) (Payne et al., 1997
; Nagy et al., 1998
).
Studies in other species have shown a relationship between the timing of completion of the first cell cycle and subsequent developmental potential. In mice and cattle, early onset of first cleavage is associated with increased blastocyst formation and implantation (McLaren and Bowman, 1973; Grisart et al., 1994
; Lonergan et al., 1999
). The suggestion that such an association might also exist in human embryos comes from the finding that patients who produced early cleaving (EC) embryos had higher pregnancy and implantation rates than those who did not (Shoukir et al., 1997
; Sakkas et al., 1998
). However, an unequivocal correlation between time from insemination to first cleavage and embryo development potential has not yet been established, since the studies to date (Shoukir et al., 1997
; Sakkas et al., 1998
) have preferentially replaced EC embryos when available. Any differences in the implantation potential of EC and non-early cleaving (NEC) embryos in the population of patients generating both embryo types were therefore not examined. Also, it is possible that other aspects of fecundity, such as improved endometrial receptivity, may account for the improved treatment success of patients generating EC embryos compared with patients with only NEC embryos.
Given its ease of application and non-subjective nature, we were interested to explore further the usefulness of early cleaving as a marker of embryonic viability. Here, we report the findings of a study in which we compared blastocyst formation in vitro of embryos that cleaved within 25h of insemination with those that cleaved later. Because the early cleaving status of each embryo was withheld until after completion of the embryo transfer, we were able to evaluate whether there was a natural bias in favour of selecting EC embryos for transfer based on morphological criteria on day 2 of development.
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Materials and methods |
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Treatment regime
Ovulation was induced using a standard protocol of GnRH analogue (Suprefact; Hoechst, Hounslow, UK) and FSH (Metrodin HP or Gonal F; Serono, London, UK) at a daily dose of 150300 IU for 1015 days, followed by 5000 or 10 000 IU HCG, (Profasi; Serono). Follicles were aspirated 3840 h after HCG administration, using ultrasound guidance. Retrieved oocytes were transferred to individual 100 µl droplets of oocyte culture medium (OCM) under mineral oil (Sigma, Poole, Dorset, UK). OCM consisted of Earle's balanced salt solution (EBSS; Life Technologies, Paisley, UK) supplemented with 25 mmol/l sodium bicarbonate (Sigma Hybri-max), 0.5mmol/l sodium pyruvate (tissue culture grade; Sigma, UK), 10% (v/v) of a 4.5% solution of human serum albumin (HSA; Immuno Ltd, Sevenoaks, Kent, UK), 10 µg/ml gentamycin sulphate (ICN; Thame, Oxfordshire, UK) and 100 IU/ml benzylpenicillin sodium (Crystapen; Brittania Pharmaceuticals, Redhill, Surrey, UK).
Sperm for insemination were isolated by centrifugation at 200 g on a discontinuous density gradient composed of 90 and 45% Percoll solutions in HEPES-buffered EBSS (Percoll; Pharmacia, Sweden; HEPES; Sigma). Between 25 000 and 50 000 sperm were added to each oocyte at 4143 h after HCG administration (day 0). At 1820 h post-insemination (day 1) the oocytes were mechanically denuded of their cumulus cells and those showing two pronuclei were transferred to individual 100 µl droplets of embryo culture medium (ECM) under mineral oil. ECM comprised EBSS supplemented with 15% (v/v) of a 4.5% solution of human serum albumin (HSA) and 0.5 mmol/l sodium pyruvate. All embryos were examined at 24.525.5 h post-insemination and those which had divided to become two cells were termed early cleaving (EC) embryos and those which had not yet divided were termed non-early cleaving (NEC) embryos.
On day 2, at 4248 h post-insemination, each embryo was assessed for cell number and assigned a morphology score between 0 and 1.0. The latter value was a semi-quantitative assessment of embryo quality, taking into account the degree of fragmentation, uniformity of blastomere size and cytoplasmic appearance. Up to three of the fastest dividing and highest scoring embryos were selected for transfer. The timing of first cleavage of the embryos was unknown to the operator at the time of transfer, and therefore did not influence the selection process.
Blastocyst culture
Following transfer of the best quality embryos, EC and NEC embryos from individual patients were pooled and cultured in separate wells of a 4-well dish (Nunclon, Life Technologies, Paisley, UK). The number of embryos cultured in each well ranged from 17 (mean = 2.3) and 113 (mean = 3.3) for EC and NEC embryos respectively. Each well contained 0.75 ml of Dulbecco's modified Eagle's medium and Ham's F-12 medium (1:1), supplemented with 2% Ultroser G (all from Life Technologies), overlaid with 0.25 ml oil. Embryos were inspected on day 7 for development to the blastocyst stage. Only expanded, hatching or hatched blastocysts were recorded. All incubations were performed at 37°C in a humidified environment of 5% CO2 in air.
Statistical analysis
Statistical evaluations used were contingency table (2) analyses for comparison of proportional values and two-sample Student's t-test for comparison of mean values.
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Results |
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Discussion |
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Our results also indicate that there is no natural bias in favour of selecting EC embryos for transfer. Consistent with this, we found that the best quality NEC embryos, i.e. those selected for transfer, were morphologically indistinguishable from the best quality EC embryos. However, this was not the case among the embryos that were not selected for transfer. Within this population, EC embryos had significantly higher cell numbers and morphology scores than NEC embryos regardless of whether they originated from group A or group B patients. Although NEC embryos produced by group A patients had significantly more cells than those of NEC embryos produced by group B patients, this did not lead to improved blastocyst formation.
A positive correlation between early onset of cleavage and blastocyst formation has also been reported in mice (McLaren and Bowman, 1973) and bovine embryos (Grisart et al., 1994
; Lonergan et al., 1999
). In these studies, the blastocysts with early cleavage were found to have more cells than their later cleaving counterparts (McLaren and Bowman, 1973
; Lonergan et al., 1999
). This was found to be attributable to the differences in the timing of first cleavage rather than to differences in the rate of progression of subsequent cell cycles (McLaren and Bowman, 1973
).
How might the timing of first cleavage be linked to blastocyst formation? The transition from fertilized oocyte to 2-cell embryo relies upon a highly regulated sequence of cell cycle events, which are initiated by sperm-induced, repetitive transient increases in oocyte free calcium concentration (Kline and Kline, 1992). There is evidence from studies in which mammalian oocytes were activated by pulsatile electrical stimulation that the dynamics of these calcium signals influence (i) the time course of pronuclear formation (Vitullo and Ozil, 1992
), which marks entry into G1 of the first cell cycle, (ii) the ability to undergo blastocyst formation (Ozil, 1990
), and (iii) implantation (Ozil and Huneau, 2001
). It has also been reported that the G2/M transition in the first cell cycle of mouse zygotes is dependent upon a calcium-releasing activity acquired by the pronuclei during fertilization or activation (Kono et al., 1996
). Thus, it could be hypothesized that asynchrony between zygotes in the timing of first cleavage and variability in their capacity to undergo blastocyst formation may be due to differences in the ability of individual sperm to stimulate calcium transients, and/or differences in the ability of oocytes to respond to that stimulus. The finding that oocytes acquire the ability to undergo repetitive calcium transients during their maturation process (Carroll et al., 1994
; Herbert et al., 1997
) suggests that oocyte maturity may be an important determinant of the timing of first cleavage and subsequent developmental potential.
A possible alternative or additional mechanistic link between timing of first cleavage and blastocyst formation lies in the fidelity of DNA replication. The duration of S phase of the first cell cycle has been shown to influence blastocyst formation. A longer S phase in association with a shorter G1 in the case of bovine embryos (Comizzoli et al., 2000), or shorter G2 in the case of mouse embryos (Schabronath and Gartner, 1988
), gives rise to improved blastocyst formation. The duration of S phase is paternally regulated (Schabronath and Gartner, 1988
; Comizzoli et al., 2000
). However, regulation of the timing of entry into S phase appears to differ between species, being regulated by maternal factors in mice (Schabronath and Gartner, 1988
) and paternal factors in cattle, in a manner that, in hamsters at least, is not dependent upon sperm nuclear decondensation (Naish et al., 1987
). It is conceivable that zygotes with shorter S phases are predisposed to incomplete or aberrant DNA replication. This is unlikely to be compatible with normal development to the blastocyst stage and could impose a delay in progression through G2 and M phase of the first cell cycle by activating a DNA structure checkpoint (Nigg, 2001
), such as has been identified in mouse zygotes (Fulka et al., 1999
).
In conclusion, our analyses show that in humans, as in other species (McLaren and Bowman, 1973; Grisart et al., 1994
; Lonergan et al., 1999
), early onset of first cleavage is associated with increased blastocyst formation. Given its ease of application and lack of scope for subjectivity, early cleaving could potentially be used as an additional marker of viability when selecting embryos for transfer. This would be especially useful in cases where numerous good quality embryos are produced and/or the risk of multiple pregnancy is increased. However, it will be important to establish whether the increased blastocyst formation of EC embryos equates to increased implantation potential. While blastocyst formation represents an important milestone in embryonic development, it is not necessarily synonymous with viability (Van Blerkom, 1997
). Although our data suggest that ability to develop to the hatched blastocyst stage may correlate with implantation potential, an unequivocal correlation between implantation potential and timing of first cleavage awaits collection and analysis of sufficient homologous data from group A patients.
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Notes |
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2 To whom correspondence should be addressed. E-mail: mary.herbert{at}ncl.ac.uk
Submitted on April 4, 2001; resubmitted on July 2, 2001
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References |
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accepted on October 12, 2001.