1 Instituto Valenciano de Infertilidad (IVI), Plaza Policía Local, Valencia and 2 Department of Paediatrics, Obstetrics and Gynaecology, University of Valencia, Valencia, Spain
3 To whom correspondence should be addressed at: Instituto Valenciano de Infertilidad, Policía Local 3, 46015 Valencia, Spain. e-mail: c.rubio{at}ivi.es
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Abstract |
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Key words: age/chromosomal abnormalities/ pronuclear morphology/nucleolar precursor bodies
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Introduction |
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The morphology of human embryos is an important parameter that seems to be related to chromosome anomalies. It has been shown that dysmorphic and slow-developing or arrested embryos exhibit significantly more polyploidy and mosaicism than normally developing human embryos (Munné et al., 1995; Márquez et al., 2000
). Increased incidences of aneuploidy and multinucleation have also been observed in embryos with a high degree of fragmentation and in those with irregular or unevenly sized blastomeres (Hardarson et al., 2001
).
With regard to human embryo development, only 25% of aneuploid embryos reach blastocyst stage compared with 62% of euploid embryos (Rubio et al., 2003
). Interestingly, a low percentage of monosomies are found at blastocyst stage, and an important percentage of trisomic embryos achieve blastocyst stage, which confirms observations in spontaneous abortions (Sandalinas et al., 2001
; Rubio et al., 2003
). Some aneuploid human embryos do reach blastocyst stage, and the transfer of such abnormal embryos to the uterus may be in part responsible for the lack of pregnancy and implantation in some IVF patients. Efforts have been made to find an adequate criterion for selecting the best embryo for transfer, thereby reducing the number of embryos that are transferred without affecting success rates (Alikani et al., 2000
). Thus, a recently introduced parameter in embryo selection is the pronuclear score. This is the assessment of pronuclear zygote morphology and the evaluation of the number and distribution of nucleolar precursor bodies (NPB). Embryo quality has also been correlated with the presence of a clear cortical zone (Payne et al., 1997
). In this respect, one group (Scott and Smith, 1998
) performed pronuclear embryo transfers and found that zygotes with perinuclear condensation were associated with higher implantation rates. In a recent report, these authors found that cytoplasmic halo-negative zygotes displayed slow embryo development, poor morphology and lower blastocyst formation (Scott, 2003
).
Embryos with a good pronuclear score (halo positive + PNs aligned + similar number and polarized NPB) have shown better implantation potential than embryos without this pronuclear pattern, both in retrospective (Payne et al., 1997; Scott and Smith, 1998
) and prospective (Scott and Smith, 1998
; Tesarik and Greco, 1999
; Ludwig et al., 2000
; Scott et al., 2000
; Wittemer et al., 2000
) studies. Furthermore, abnormal patterns of pronuclear morphology are correlated to a higher rate of cleavage arrest, as well as lower blastocyst development (Scott and Smith, 1998
; Tesarik and Greco, 1999
; Scott et al., 2000
; Wittemer et al., 2000
; Balaban et al., 2001
; Fisch et al., 2001
; De Placido et al., 2002
; Rienzi et al., 2002
; Zollner et al., 2002
). Current studies have found that zygotes with different pronuclear sizes present a significantly higher incidence of both embryo cleavage arrest and mosaicism in day 3 embryos than do normal zygotes (Munné and Cohen, 1998
; Sadowy et al., 1998
) and lower developmental potential (Scott, 2003
).
Since both the chromosomal status of the embryo (Sandalinas et al., 2001; Rubio et al., 2003
) and pronuclear patterns seem to be related to embryo cleavage ability, a link may exist between the pronuclear score and chromosome constitution of human embryos. Therefore, in the present study the aim was to investigate whether a correlation existed between pronuclear morphology during the zygote stage and chromosome abnormalities in day 3 human embryos. Embryo development up to blastocyst stage was also studied with respect to pronuclear morphology patterns.
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Materials and methods |
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Indications for PGD were as follows: 31 couples with recurrent miscarriage (RM) of unknown aetiology, 32 couples with repetitive implantation failure (IF) following IVF or ICSI, 16 couples with aneuploidy screening (AS) due to advanced maternal age or mixed causes, and two couples with a high incidence of chromosomal abnormalities after fluorescence in-situ hybridization (FISH) analysis in the spermatozoa. Stimulation, oocyte retrieval and ICSI procedures were performed as described previously (Pellicer et al., 1999).
Assessment of zygote and embryo morphology
Pronuclear zygote morphology was assessed at 1618 h post-ICSI at x40 magnification under an inverted microscope. Two groups were formed based on pronuclei size: those of equal or very similar size (group A); and those of different sizes (group B). In each group, zygotes were subdivided into three categories according to the number, distribution and synchrony of NPB (Figure 1): subgroup I, pronuclei with 34 polarized NPB; subgroup II, 57 synchronic polarized NPB or 710 NPB distributed randomly throughout the pronucleus; and subgroup III, morphologies other than those of groups I or II (asynchronic NPB polarization, alignment of more than 7 NPB at the point of contact of the two pronuclei, a difference in more than three NPB between pronuclei, random distribution of 4 NPB in both pronuclei). The presence of a cytoplasmic halo was also evaluated in 537 zygotes, classifying zygotes as halo-positive when they showed a perinuclear condensation of the cytoplasm, and halo-negative when this polarization of the cytoplasm did not exist.
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Embryo coculture
After removal of the cumulus, oocytes, zygotes and embryos were cultured in 50 µl microdroplets of IVF medium (Scandinavian IVF; Gothenburg, Sweden) until day 2. From day 2 to day 5, embryos were kept individually in coculture with epithelial endometrial cells (EEC) as follows: on day 2, when at the 2- to 4-cell stage, embryos were placed in coculture in the presence of EEC with 1 ml IVF:CCM (1:1) (Scandinavian IVF); on days 3, 4 and 5, embryos were cocultured with 1 ml CCM. Ultrasound-guided embryo transfers were performed on day 5 using either Wallace (SIMS Portex Limited, Kent, UK) or Gynétics (Gynétics Medical Products, Hamont-Achel, Belgium) catheters.
Embryo biopsy and FISH protocol
Embryo biopsy was performed on day 3 developing embryos with 5 nucleated blastomeres and a
25% degree of fragmentation. In embryos with
7 cells, two blastomeres were biopsied and in embryos with <7 cells only one blastomere was retrieved. Two cells were analysed in 318 embryos, and in the remaining 251 embryos one cell was analysed. Mosaic embryos were classified as those having different FISH results when two cells were analysed (318 embryos). The risk of FISH errors was assumed, but the risk was considered to be homogeneously distributed in all groups and did not interfere in the statistical analysis of the results.
For the biopsy, embryos were placed on a droplet containing Ca2+- and Mg2+-free medium (EB-10; Scandinavian IVF). Tyrodes solution (ZD-10; Scandinavian IVF) was used to perforate the zona pellucida. One or two blastomeres were withdrawn with a bevelled aspiration pipette and individually fixed under an inverted microscope with methanol:acetic acid (3:1), using a slightly modified Tarkowskis protocol without hypotonic pretreatment (Tarkowski, 1966; Rubio et al., 2003
).
In all blastomeres from the 569 day 3 embryos, seven chromosomes were analysed using FISH in three steps. A first hybridization round was performed using locus-specific probes for chromosomes 13 and 21. In a second round, after signal elimination, blastomeres were hybridized with a centromeric probe for chromosome 16 and a locus-specific probe for chromosome 22. Finally, in a third round, a triple FISH was performed with centromeric probes for chromosomes X, Y and 18 (all probes commercially available from Vysis Inc., Downers Grove, IL, USA).
The percentage of abnormal embryos in each group was estimated as the number of affected embryos divided by the number of embryos analysed, regardless of the probe employed. Embryos were classified as normal, abnormal, mosaic (two blastomeres from the same embryo displaying different results) and multinucleated (two or more nuclei/blastomere). To analyse embryo development, the following groups were established: embryos arrested at days 2 or 3; embryos arrested at days 4 or 5 without compaction or cavitation; and embryos at morula or blastocyst stage on day 5. Abnormal embryos were classified as carriers of autosomal monosomies, carriers of monosomy X, carriers of autosomal trisomies and trisomies for sex chromosomes, those with combined monosomy and trisomy and haploid, triploid, tetraploid and mosaic embryos.
Statistical analysis
For statistical comparison between groups, chi-square analysis and Fishers exact test were used to compare the percentages of chromosomally abnormal embryos and arrested embryos at different stages. A P-value <0.05 was considered statistically significant. The statistical analysis was carried out using the Graphpad Instat v. 2.05a package (Graphpad Software, San Diego CA, USA).
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Results |
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The relationship between zygote patterns and early embryonic development until day 5 is detailed in Figure 2a and b. In patients aged 37 years (Figure 2a), the percentage of zygotes arrested on days 2 and 3 was not statistically different in the five PN patterns. Blastocyst/morula rates showed statistical differences between groups AI and BIII (42.1 versus 16.7; P = 0.0085), AII and BIII (50.0 versus 16.7; P < 0.0001), and AIII and BIII (42.2 versus 16.7; P = 0.0017). The number of zygotes reaching morula and blastocyst stage was greater in group A (45.6%) than in group B (19.6%) (P = 0.0003). The percentages of embryos arrested on days 4 and 5 varied significantly (P = 0.0493) between zygotes with similar pronuclear size (group A) and zygotes with unequal pronuclear size (group B) (27.4 versus 39.2). Statistical differences among groups were not observed in zygotes from couples aged >37 years (Figure 2b).
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The incidence of chromosomally abnormal embryos is evaluated for each PN pattern, according to the maternal age groups, in Tables I and II. A stronger correlation of PN patterns with chromosomal abnormalities was observed in patients aged 37 years (Table I). Statistical differences were observed when pattern AI (51.4) was compared with pattern AII (71.5%, P = 0.0280), pattern AIII (72.7%, P = 0.0024), pattern BIII (88.9%, P = 0.0077) and total B (73.1%, P = 0.0247). When the maternal age was >37 years, the incidence of chromosomal abnormalities was independent of the pronuclear pattern (Table II). A detailed description of the chromosomal abnormalities found in each subgroup is shown in Table III.
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Discussion |
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In patients aged >37 years, oocyte agingwith all that this impliesseems responsible for poor reproductive outcome. It is known that implantation rates decrease after the age of 37 years (Van Kooij et al., 1996), and age has been highlighted as the most influential factor in reproductive outcome affecting pregnancy and miscarriage rates (Ron-El et al., 2000
; Spandorfer et al., 2000
). The success of oocyte donation programmes in older patients has given credence to the idea that the endometrium retains normal receptivity (Remohí et al., 1997
). On the other hand, a high incidence of chromosomal abnormalities has been observed in preimplantation embryos of patients aged over 37 years (Munné et al., 1995
; Gianaroli et al., 1999
; Pellicer et al., 1999
). The incidence of non-disjunction of bivalent chromosomes during oogenesis has been shown to increase with age (Hassold and Sherman, 2000
). The majority of maternal aneuploidies originate from non-disjunction during the oocytes first meiotic division (Jacobs and Hassold, 1995
), which occurs before fertilization and, therefore, does not affect pronuclear morphology.
In previous studies in IVF cycles, closely apposed pronuclei, with nucleoli aligned at the pronuclear interphase and perinuclear condensation of the cytoplasm were considered to provide good prognosis pronuclear patterns for implantation. These conclusions were drawn from results obtained after transfer on day 1, from which a pronuclear scoring system was devised (Scott and Smith, 1998). Similar results were observed in ICSI cycles, with higher pregnancy rates when day 3 embryos with good prognosis patterns were selected for transfer (Tesarik and Greco, 1999
). These findings provided new criteria for selecting the best embryos for transfer, particularly when a large number of similarly good quality embryos was available for transfer at cleavage stage.
Most recent studies have based their pronuclear scoring systems solely on nucleoli distribution (Wittemer et al., 2000; Balaban et al., 2001
; Fisch et al., 2001
; Montag and Van der Ven, 2001
). Yet unequal size or distance between the pronuclei have also been associated with poorer embryo quality and lower developmental rates and with a higher incidence of multinucleation (Sadowy et al., 1998
; Scott et al., 2000
; Zollner et al., 2002
; Scott, 2003
). In the present study, most of the zygotes showed similar PN size (group A), with only 8.1% of B type zygotes (unequal PN size). Similar results have been described by others (Scott, 2003
), with 10.4% of zygotes showing Z4 patterns (unequal size or non-aligned nuclei). Furthermore, multinucleation has been correlated to higher rates of mosaicism and chromosomal abnormalities in preimplantation embryos (Kligman et al., 1996
). In fact, in one study, a significantly higher incidence of mosaicism was found in day 3 embryos from zygotes with dysmorphic pronuclei than in those from zygotes with normal pronuclear morphology (Sadowy et al., 1998
). Likewise, in the present study a higher incidence of chromosomal abnormalities was found in embryos originating from pronuclei with different sizes, and this value increased when the parameter of NPB distribution was included. In the present study, among patients aged
37 years, there was a trend towards a higher incidence of chromosomal abnormalities in embryos from zygotes with different pronuclei sizes, and statistically significant differences were found when a second parameterthe number and distribution of NPBwas added. In other words, embryos from zygotes with equal pronuclear size and synchronized and polarized nucleoli suffered significantly less chromosomal abnormalities than embryos derived from zygotes with different PN size and asynchronic NPB. Comparisons of the second parameter (NPB distribution) as an isolated variable were impeded by the lack of zygotes with different pronuclei sizes and synchronized and polarized nucleoli.
Another parameter evaluated in PN morphology is the appearance of a cytoplasmic halo during PN formation. This is due to the contraction of organelles from the cortex to the centre of the oocyte, which leaves a clear cortical zone (Payne et al., 1997). Previous studies have not clearly demonstrated the effect of this parameter on embryo development (Demirel et al., 2001
), although the presence of perinuclear condensation has been associated with higher implantation rates (Scott and Smith, 1998
). Others (Balaban et al., 2001
) found a higher percentage of good morphology embryos in halo-positive zygotes, but did not observe any statistical differences in pregnancy and implantation rates. The present data showed a significantly higher blastocyst rate in halo-positive than in halo-negative zygotes in patients aged
37 years. The incidence of chromosomal abnormalities in day 3 developing embryos was slightly increased in halo-negative zygotes, although this difference was not statistically significant. This effect was not observed in patients aged >37 years.
Oocyte cytoplasmic immaturity and sperm decondensation defects could lead to the development of abnormal pronuclear patterns (Tesarik and Kopecny, 1989; Rienzi et al., 2002
). A study performed in couples with two consecutive oocyte donation cycles showed that certain ICSI sperm samples repeatedly produced high proportions of zygotes with abnormal PN patterns. This effect was not thought to be related to any of the conventional sperm parameters; rather, the authors attributed it to a minor gene activity of the male pronucleus, to the sperm centrosome or to the sperm-derived oocyte-activating factor (Tesarik et al., 2002
). In the present study, statistical differences were observed in the percentage of zygotes derived from normozoospermic samples between groups A and B of the younger patients, although oocyte contribution could not be completely ruled out. In a recent study, a correlation between the source of spermatozoa (ejaculate and testicle) and pronuclear patterns could not be established (Demirel et al., 2001
). However, other authors found that the microinjection of spermatids (Tesarik and Mendoza, 1996
; Kahraman et al., 2002
) or testicular sperm into mature oocytes (Kahraman et al., 2002
) could lead to zygotes with poor prognosis PN patterns.
The present study, and the results of other published studies, confirm that when there exists polarization of NPB in both pronuclei and the pronuclei are of similar size, embryo quality and development is improved (Sadowy et al., 1998; Scott and Smith, 1998
; Tesarik and Greco, 1999
; Scott et al., 2000
; Balaban et al., 2001
; De Placido et al., 2002
; Kahraman et al., 2002
; Rienzi et al., 2002
; Zollner et al., 2002
; Scott, 2003
). As has already been mentioned, chromosomal abnormalities have a detrimental effect on preimplantation development. There fore, it is not a coincidence that the PN pattern displaying a more favourable embryo development has also been associated with a lower rate of chromosomal abnormalities (Sadowy et al., 1998
; Kahraman et al., 2002
). On the other hand, as also suggested by authors combining PN scoring with day 2/day 3 embryo scoring, the worst zygote patterns are the most accurate indicators of a poor IVF/ICSI outcome (De Placido et al., 2002
).
It can be concluded that pronuclei of different size and asynchronic NPB distribution negatively affect embryo development, resulting in higher rates of chromosomal abnormalities in day 3 embryos. This embryo selection for improving IVF outcome, based on PN morphology, is useful mainly in patients aged 37 years. In patients aged over 37 years, PGD for the most common aneuploidies is the best approach for limiting the risk of transmitting chromosomal abnormalities to the offspring.
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Acknowledgements |
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Submitted on November 26, 2002; resubmitted on May 14, 2003; accepted on July 18, 2003.