1 Centre for Reproductive Medicine, University Hospitals Coventry and Warwickshire NHS Trust, Clifford Bridge Road, Coventry CV2 2DX and 2 Department of Public Health and Epidemiology, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
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Abstract |
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Key words: blastocyst/embryo transfer/human/multiple pregnancy/questionnaire
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Introduction |
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After IVF, multiple pregnancies arise mainly when more than one embryo is transferred to the uterus. This is done to increase the chances of at least one embryo implanting and resulting in pregnancy, however, the outcome is unpredictable and multiple pregnancies often result. In the UK, up to three embryos may be transferred concurrently, but concerns about high order multiple pregnancies prompted the Royal College of Obstetricians and Gynaecologists (RCOG) to recommend a maximum of two, in women aged <40 years (Royal College of Obstetricians and Gynaecologists, 2000). The Human Fertilisation and Embryology Authority (HFEA) now requires exceptional circumstances to be documented before three embryos are transferred, and recommends not more than two as routine (Human Fertilisation and Embryology Authority, 2001
).
A trade-off exists between the risk of multiple pregnancy and prospects for pregnancy itself. This trade-off could be moved further against multiple pregnancy by selecting the most viable embryos (Shoukir et al., 1997; Scott and Smith, 1998
). One suggestion, `blastocyst culture', is the prolonged culture of embryos in vitro for ~5 days, by which time the least viable may have succumbed, leaving the most competent available for transfer (Steptoe et al., 1971
). This is reputed to mimic the physiological process (Buster et al., 1985
), and in many species is the most successful approach to IVF. In humans, blastocyst culture has been reported to increase substantially the implantation rate per embryo transferred, provided that newer purpose designed culture media are used [new: (Gardner et al., 1998
; Huisman et al., 2000
; Milki et al., 2000
) old: (Bolton et al., 1991
; Scholtes and Zeilmaker, 1996
)] yet it remains highly controversial for several reasons. Extended culture is an artificial means of selection and may not reflect in-vivo competence. The safety of prolonged culture has not yet been established (Ménézo et al., 1999
; Sinclair et al., 2000
). The outstanding results obtained by the most successful proponents have generally not been reproduced elsewhere (Coskun et al., 2000
). Crucially, even using the latest developments in culture medium, only ~50% of fertilized oocytes survive to become blastocysts. This could result in a proportion of patients having no blastocysts to transfer, despite having embryos available at an earlier stage. Nevertheless, if the potential to select more competent embryos for transfer could be realised, a reduction in the number of embryos transferred, whilst maintaining an acceptable pregnancy rate, would offer a valuable prize of reduced risk of multiple pregnancies. As a bonus, there could be major savings for health services. It is our belief that a properly conducted trial is essential to provide an evidence base on whether the potential benefits of blastocyst culture can be realised in assisted conception centres across the country.
In preparation for a multicentre clinical trial comparing blastocyst culture with the current common practice of embryo transfer on day 2 or 3, we wished to explore the perceptions of patients and health care personnel on the potential benefits and risks of blastocyst transfer, in particular with reference to the above-mentioned trade-off between multiple pregnancy and any pregnancy. This would provide useful information on the likelihood of patients wishing to participate in the trial, and the likelihood of participating clinics engaging actively in recruitment. The findings have provided insights and highlighted differences in the priorities of patients and those involved in their treatment.
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Materials and methods |
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The respondents were given information based upon recently published literature, before answering the questions. The base-line information was as follows: Once fertilization is assured, a normal IVF or ICSI cycle has a 99% chance of embryos being transferred on day 2 or 3 (local audit) and blastocyst culture has about a 90% chance of embryos being transferred on day 5 or 6 (Jones et al., 1998; Marek et al., 1999
). The average risk of a pregnancy being twins with current practice was taken to be 28% and triplets 3% (Human Fertilisation and Embryology Authority, 1999
). We assumed that about 50% of embryos would become blastocysts in vitro (Gardner et al., 1998
; Jones et al., 1998
; Cruz et al., 1999
). The assumption in Questions 2 and 3 that blastocyst transfer might result in a 7% increase in pregnancy rate was based upon a straw poll by telephone of clinicians and scientists in 20 UK assisted conception centres (G.Hartshorne, unpublished data). A copy of the full questionnaire is available from the corresponding author.
The questionnaire was presented to three groups. The provision of identifying or non-identifying information by the respondents about themselves was optional.
Group 1. Prospective, current and past patients attending an open meeting of the Centre for Reproductive Medicine Patient Support Group. An invitation was extended to G.H. to address the meeting, at the recommendation of the Quality Focus Group. Those present were invited to complete the questionnaire, after a brief informal talk on our proposal for a trial of blastocyst culture, including information on the chances of multiple pregnancy and its consequences. The session was relaxed and patients asked questions freely. The responses were discussed anonymously with the Quality Focus Group to facilitate interpretation and to provide feedback to the Patient Support Group.
Groups 2 and 3. Delegates attending relevant conferences were offered the questionnaire for completion during a slot in the programme. The conferences were a symposium on blastocyst culture, hosted by Birmingham Women's Hospital, and the winter workshop of the British Fertility Society (BFS, St Thomas's Hospital, London).
The responses were allocated into the following groups for analysis: patients, scientists, clinicians (medical doctors) and nurses.
Answers to the quantitative questions were analysed statistically using the non-parametric Kruskal Wallis test and pxq contingency tables (Campbell, 1989). Qualitative issues arising from the open questions were assessed by counts of like answers.
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Results |
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The information presented voluntarily showed that the patient group included three who had had successful treatment previously, one resulting in twins. Three of the other 17 were embarking upon their first IVF or ICSI cycle, the remainder had had 19 previous IVF or ICSI attempts.
The respondents attending conferences comprized three fertility nurses, 33 embryologists, 16 medical doctors (clinicians) and two who did not specify their job titles. These two were included in the clinician group since they were attending the BFS meeting, from which all the other respondents were clinicians. The small number of nurses (three) precluded statistical evaluation. All the clinicians practised assisted conception, while eight did and five did not practice obstetrics. Thirteen clinicians and 22 embryologists were from units that had attempted blastocyst culture.
Open questions
The positive and negative points volunteered in response to Question 1 (general feelings about blastocyst culture) are summarized in Table I. Patients produced a significantly (P < 0.05) greater proportion of positive replies than specialists, with embryologists producing nearly equal numbers of positive and negative comments. A total of 41% of the patients' positive remarks related to the higher chance of pregnancy, compared with 23 and 14% of embryologists and clinicians respectively. None of the patients remarked upon a reduction in multiple pregnancy rate as an advantage of blastocyst culture, whereas this accounted for a quarter of the comments from health care professionals. The increased availability of information about embryo development resulting from blastocyst culture was recognized by all groups. The main disadvantages noted were the increased chance of having no embryo transfer and the need for further information and follow-up of children. Workload, resource and technical concerns featured strongly among the embryologists' replies.
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Responses to Question 4 showed that 59% of patients and 72% of healthcare professionals would not be deterred from the trial by a 10% risk of no blastocyst transfer (Table III).
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Discussion |
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The present study has confirmed that patients are more willing to accept the risks of multiple pregnancy in prospect than their carers (Gleicher et al., 1995). This significant difference was found notwithstanding a lack of difference on several other questions. Infertile patients are clearly aware of the risks of multiple pregnancy but many choose to accept them, on behalf of themselves and their potential children. It is debatable whether patients may be considered fully able to take an objective view upon this point as their perception of multiple pregnancy may be affected by their personal situation. Whether the same view would be taken in retrospect is not known. However, these patients would be considered competent to take responsible decisions in other respects, including consent or otherwise to medical treatment for themselves and any young children they may already have.
Clinicians may be affected by the perinatal prognosis of multiple pregnancies. In addition, it is possible that health care providers in general and clinicians in particular may be more aware of literature from the last 20 years indicating that rearing twins, and more especially higher order births, is more stressful than might be apparent to someone who has not experienced the event. However the qualitative responses received about multiple pregnancy (Question 5) did not indicate any difference in risk-awareness among the groups, 54% of patients and embryologists and 44% of clinicians indicating through their answers that they are aware of the risks associated with multiple pregnancy. However, the lack of evidence in many answers may relate more to the way in which respondents expressed themselves than to a lack of risk awareness. Patients attending this centre receive a pack including information about multiple pregnancy before their first consultation, and this is reinforced with an information sheet at the consultation and information counselling sessions, all of which occur before treatment commences. Our patients should therefore be well informed on this subject. It would be interesting to see how consumer views vary according to knowledge of the clinical and psychological effect of multiple pregnancy, but our results in this study suggest that there may be a genuine difference in values on this point between patients and those involved in their treatment. Furthermore, the literature on the psychosocial effects of rearing twins or higher multiples focuses on the early years of life and patients may be taking a `life-course' view. The ideal outcome from a clinical perspective would be a healthy singleton child, yet one can understand a couple hoping for a ready made family and `two for the price of one', in the present situation where IVF treatment is usually at a price.
Whether these risks are acceptable to society is another matter, when considerable public expenditure is required to care for iatrogenic multiple pregnancies (Dunn and McFarlane, 1996; Hidlebaugh et al., 1997
; Wolner-Hanssen and Rydhstroem, 1998
). Moreover, the legal requirement to consider the welfare of the child places an additional responsibility on the service provider (Human Fertilisation and Embryology Act, 1990
). Clinicians and embryologists both demonstrated concern about the outcome of pregnancy, and it is important that we should work for future improvements. However, the main issue for patients facing treatment remains their chance of a pregnancy, and philosophically we are uneasy in the notion that parents should be coerced to accept societal or professional limitation on all but the most extreme actions they may choose.
The input of results from the questionnaire produced a well informed discussion and resulted in our moving towards a pragmatic trial design. As shown in Table II, patients felt that those with previous attempts and older/poor prognosis patients should be included, and were more relaxed about transfers of more than two embryos than the other groups (Table V
). A quarter of clinicians supported the transfer of embryos singly (Table V
) whilst requiring a pregnancy rate of at least 20% to be maintained (Table VII
).
It has been previously suggested that a reduction from three to two embryos transferred in patients for whom there is a choice will not affect pregnancy rates (Templeton and Morris, 1998; Royal College of Obstetricians and Gynaecologists, 2000
). This retrospective interpretation of national statistics has not been challenged by a prospective randomized controlled trial, and some evidence is contradictory (Gerris et al., 1999
). The number of embryos transferred beyond which no further increase in pregnancy rate would occur has not yet been established with certainty, but may be at least three (Fishel et al., 1985
; ASRM/SART, 2000
). Fewer embryos are normally transferred to patients perceived to be more fertile in view of a younger age, previous history of pregnancy, aetiology of infertility and lower likelihood of age-related reduction in embryo viability, rendering the national data uncontrolled, and tending to equalise the pregnancy rates of two embryo transfers (good prognosis patients) and three embryo transfers (poor prognosis patients). Changes in the implantation rate due to e.g. blastocyst culture, would also affect such calculated predictions, i.e., if the theory that blastocysts have higher implantation rates per embryo is confirmed, then the alteration in pregnancy rate effected by each additional embryo transferred would be more marked.
An interesting variety of responses was received on the best method of starting a new treatment, indicating broad diversity of opinion on a topic of some importance and perhaps reflecting the complexity of balancing the needs of the individual patient who may be paying for their own treatment against the need to design appropriate trials. Assisted conception is noted for the lack of control with which new methods are often introduced (Vandekerckhove et al., 1993), and the current legislative arrangements in the UK require that any new technique is shown to be safe before its introduction to patient treatment. As a result, equipoise may be lost before the treatments are ready and available for trial in this country. It is unfortunate that our application for a multicentre RCT was rejected, rendering more likely the introduction to the UK of another assisted conception technique before its scientific evaluation.
A further concern is the paucity of long-term follow-up data on children resulting from various in-vitro procedures, including blastocyst culture. Funding of such expensive studies is a major issue for a discipline where state funding is restricted and most patients are paying for their own treatment.
Blastocyst culture is unlikely to be a suitable treatment for every infertile couple, and many questions persist about its most useful application and the reality of the benefits that it promises. Nevertheless, our investigation has shown that patients and centres would be willing to participate in a trial and the majority would not be put off by a 10% risk of all embryos failing to survive, though a substantial minority would wish to retain the option of three embryos transferred, despite the elevated risks of multiple pregnancy.
In conclusion, our questionnaire has highlighted differences among patients, embryologists and clinicians in their perceptions of the desirability of multiple pregnancy, their preferences in certain practical aspects of treatment such as embryo transfer numbers, and their ideas on blastocyst culture and its prospective outcomes and risks.
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Appendix |
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Personal information (confidentialno names will be attributed)
For patients:
Your name:
Your age:
Your number of previous attempts:
Any special comments:
For symposium delegates:
Your name:
Your job title:
Has your unit attempted blastocyst culture?
Any special comments:
For BFS delegates:
Your name:
Your job title:
Do you practise assisted conception?
If so, has your unit attempted blastocyst culture?
Do you practise obstetrics?
Any special comments:
Open questionsPlease answer in your own words
(1) What do you think of the idea of blastocyst culture?
Good and bad points?
(2) Who should have blastocyst culture?
Age
No of embryos needed to start blastocyst culture treatment
No of previous attempts?
(3) What % increase in pregnancy rate would make blastocyst culture worthwhile?
(4) Would a 10% risk of all the embryos dying before the transfer put you off this service?
(5) What do you think about the risks of multiple pregnancy?
(6) How many embryos should be transferred?
(7) What is the best way to start a new treatment?
Quantitative questions
1. If we assume (once fertilization is assured) that a normal IVF or ICSI cycle has a 99% chance of embryos being transferred on day 2 or 3 and that blastocyst culture has about a 90% chance of embryos being transferred on day 5 or 6, please indicate the percentage increase in pregnancy rate (i.e. in addition to the standard IVF/ICSI pregnancy rate of about 25%) which you would expect before preferring blastocyst culture.
2. If blastocyst culture offered a 7% increase in pregnancy rate, what additional risk of a twin pregnancy would you be prepared to accept? (Average risk of a pregnancy being twins with current practice is about 28%).
3. If blastocyst culture offered a 7% increase in pregnancy rate, what additional risk of a triplet pregnancy would you be prepared to accept? (Average risk of a pregnancy being triplets with current practice is about 3%).
4. Patients will be asked to choose how many fertilized embryos they would want to have before they decide whether to enter the trial. A higher number gives a better chance of getting blastocysts for transfer (on average about 50% will become blastocysts but this is very variable). However, because not all of the embryos are likely to become blastocysts, the chances of having enough for a cryopreservation cycle are reduced. How may embryos would you choose as a starting point?
5. The legal maximum number of embryos which can be transferred is three, but recent guidelines from the Royal College of Obstetricians and Gynaecologists advise not more than two, to reduce the chance of multiple pregnancy which carries risks to babies and their mothers. Patients entering the trial will receive not more than two cleaving embryos or two blastocysts. Would you still want the choice of having three embryos transferred? YES/NO
6. Blastocysts are believed to have a higher chance of implanting than cleaving embryos. Depending upon the number of embryos replaced this could result in:
A: The same chance of pregnancy as now, but a lower risk of multiple pregnancy.
B: A higher chance of pregnancy than now, with the same risk of multiple pregnancy.
C: An even higher chance of pregnancy, together with an increased risk of multiple pregnancy.
D: The possibility of nearly eliminating multiple pregnancy (except identical twins) by replacing only one embryo, which would be associated with a lower chance of pregnancy. Would you prefer A, B, C or D?
7. What chance of pregnancy would you expect before you would choose single embryo transfer?
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Acknowledgements |
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Notes |
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References |
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Submitted on August 13, 2001; resubmitted on October 5, 2001; accepted on November 29, 2001.