1 University Departments of Obstetrics and Gynaecology, Jessop Hospital for Women, Leavygreave Road, Sheffield S3 7RE, 2 Department of Epidemiology and Public Health, University of Leicester, Leicester LE1 6TP and 3 Department of Molecular Biology and Biotechnology, University of Sheffield S10 2TN, UK
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Abstract |
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Key words: azoospermia/diagnostic index/germ cells/prediction/sperm retrieval
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Introduction |
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Whether or not to undertake a therapeutic sperm extraction procedure without a preliminary diagnostic testicular biopsy is debatable. Patients with non-obstructive azoospermia may undergo a prior diagnostic testicular biopsy (Silber et al., 1997; Tournaye et al., 1997
) or may have multiple therapeutic testicular biopsies scheduled with oocyte retrieval to look for spermatozoa for ICSI with no preliminary biopsy (Devroey et al., 1995
; Tournaye et al., 1996
). In cases where no spermatozoa are retrieved the couples are prepared for donor insemination. Patients with non-obstructive azoospermia may have cryptozoospermia. Hence, a careful search of the ejaculate on the day of ovum retrieval may yield some spermatozoa without resorting to testicular extraction. Nagy et al. (1995) reported an ongoing pregnancy rate of 24.5% per cycle in a study of 57 cycles of the wives of men with cryptozoospermia, a value similar to that reported for ICSI with testicular and epididymal spermatozoa or ejaculated sperm from men with normozoospermia. Cryptozoospermia may occur in 1035% (Lindsay et al., 1995
; Ron-El et al., 1997
) of patients with azoospermia, the exact proportion depending on the centrifugation and duration force used in the preparation of the semen sample. Moreover, cryptozoospermia may be intermittent, so that ejaculated spermatozoa may not be found on the day of oocyte retrieval. Conventional parameters associated with spermatogenesis, including plasma follicle stimulating hormone (FSH), testicular volume and testicular histology individually have failed to provide a good discrimination between patients who will have successful and failed testicular sperm extraction (Tournaye et al., 1996
, 1997
; Mulhall et al., 1997
). It is possible that a combination of these variables may provide a better discrimination. Algorithms and predictive models have not been developed for the treatment with testicular biopsy and ICSI of men with azoospermia due to defective spermatogenesis, probably because this type of treatment is relatively new. Such models can be used to establish diagnosis, aid counselling of patients and reduce the money spent on unnecessary investigations.
The objective of this pilot study was to explore the diagnostic value of current parameters used in the management of azoospermia, including testicular volume, plasma FSH concentration, Johnsen score and the visualization of testicular spermatids. Logistic regression analysis was used to develop a diagnostic model, based on a set of variables associated with spermatogenesis, that could be applied to individual patients, thereby limiting testicular biopsy to those patients with the best chance of yielding testicular spermatozoa.
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Materials and methods |
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Testicular sperm extraction
A piece of biopsy tissue (~5 mg) was minced into fine pieces (<1 mm3) using a pair of sterile glass microscope slides for ~25 min. The resultant suspension was transferred in PBS to a conical centrifuge tube (Falcon type, 100 mm; Fahrenheight Laboratory Supplies, Rotherham, UK), vortexed for 5 min and centrifuged for 5 min at 500 g. The pellet was resuspended in 2 ml of erythrocyte-lysing buffer (155 mM NH4Cl, 10 mM KHCO3, 2 mM EDTA, pH 7.2) and allowed to stand for 5 min. After centrifugation at 500 g for 5 min, the pellet was resuspended in Earle's balanced salt solution (EBSS) supplemented with 2.26% human serum albumin (Gibco BRL, Life Technologies, Paisley, UK) and transferred to a Petri dish containing 3 ml of EBSS. The medium was overlayered with 1 ml paraffin oil. The preparation was immediately examined under an inverted microscope equipped with Hoffman modulation contrast photomicroscopy at magnifications x200 and x400 for the presence of testicular sperm and then incubated at 37°C in an atmosphere of 5% CO2 in air for up to 72 h. This testicular culture was examined daily for the presence of testicular spermatozoa.
Histopathology
Semi-thin paraffin wax testicular tissue sections (4 µm thick) were stained with haematoxylin and eosin and then examined under a light microscope at x100 and x400 magnification using standard techniques. Testicular histology was classified into hypospermatogenesis (reduction in the number of normal spermatogenetic cells), maturation arrest (an absence of the later stages of spermatogenesis), Sertoli cell-only (the absence of germ cells in the seminiferous tubules), and tubular sclerosis (no germ cells or Sertoli cells present in the tubules). The presence of an occasional seminiferous tubule with a few spermatids in a field of seminiferous tubules that otherwise exhibited maturation arrest, Sertoli cell-only pattern or tubular sclerosis was classified as focal spermatogenesis. One hundred tubules were examined per slide and each slide was scored using the Johnsen score (Johnsen, 1970), whereby seminiferous tubules are assessed on a scale of 110, with tubules having a complete lack of cells scored as 1 and those with a maximum sperm presence (at least five or more spermatozoa in the lumen) scored as 10. In order to obtain a mean score for each patient, the number of tubules recorded at each Johnsen score point was multiplied by the corresponding Johnsen score, and the sum of all multiplications was divided by the total number of tubules recorded. The average of the scores for both testes was obtained to get the overall score for each patient. The slides were examined by three different assessors who were unaware of the results of the testicular sperm retrieval. Discussion among the assessors resolved any discrepancies and enabled a final diagnosis to be reached, so that the coefficient of variation was not calculated. Quantitative analysis of spermatid count was not performed (Silber et al., 1997
). Instead the finding of at least one round, elongating or elongated spermatid on histological sections was defined as `positive spermatid on histology'.
Design
The endocrine profile (plasma FSH, LH and testosterone concentrations) and biophysical profile (patients' age, paternal and maternal ages at birth, occupation, risk factors for azoospermia, height, weight, BMI, and the sum of volume of both testes), whether or not testicular spermatids were visualized, and Johnsen score were used as predictors of the likelihood of retrieval of at least one testicular spermatozoon. Consecutive patients were studied.
Blinding of variables and end-points
The persons performing the semen analysis, testicular sperm extraction and clinical evaluation were blinded from each other. Each of these procedures was performed by the same person on each occasion.
Statistical analysis
The end-point was retrieval of at least one testicular spermatozoon. The sensitivity (the proportion of patients with successful sperm extraction identified by the predicting test) and specificity (the proportion of patients without testicular spermatozoa correctly identified by the predicting test) of testicular volume, plasma FSH concentration, Johnsen score and presence of testicular spermatids in predicting the probability of testicular sperm extraction were calculated. The accuracy of prediction of the likelihood of successful testicular sperm extraction and the best cut-off level for the variables in predicting the likelihood of testicular sperm retrieval were assessed by the area under the receiver operating characteristic (ROC) plot (Zweig and Campbell, 1993), a plot of true positive (sensitivity) against false positive (1 specificity) rates for each possible cut-off point. Because the sensitivities and specificities were calculated separately, but using test results from two different subgroups (i.e. the sensitivity was calculated from the subgroup with testicular sperm present and specificity from the subgroup without testicular sperm), the ROC plot was independent of the prevalence of spermatozoa in the testis. The area under the ROC plot was determined statistically using the Wilcoxon and MannWhitney U-statistics (Hanley and McNeil, 1982
) (Appendix 1). The best cut-off value was considered to be that which maximized the sum of sensitivity and specificity, roughly indicated on the graph by the shortest distance between the ROC curve and the top left-hand corner (where sensitivity = 1 and specificity = 1).
Stepwise logistic regression analysis was performed with forward selection to identify which combination of the potentially predictive variables was most predictive of testicular sperm retrieval. This technique was used to develop a mathematical equation to calculate the probability of sperm extraction in any patient on the basis of his own results.
The performance of the diagnostic model was then tested in a study sample using the Jackknife technique (Efron and Gong, 1983), in order that the prediction rule used for a given patient was derived independently of that patient's own data. Jackknife technique is an estimation of the error rate of a prediction model which gives a result similar to that obtained by testing the model in a different independent population. The method consists of (i) first deleting the patients x1 from the data sample, (ii) using logistic regression analysis to recalculate the prediction rule on the basis of the remaining n x1 patients, (iii) using the new prediction rule to predict the outcome of x1 and (iv) returning the patient x1 to the group, and repeating the same procedure separately for patients x2 to xn (x40). Analysis was performed using the 50% cut-off point to ensure that the likelihood of predicting successful sperm extraction was similar to the observed success. The misclassification rate, sensitivity, specificity as well as the positive and negative predictive values of the diagnostic index were calculated.
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Results |
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Sensitivity, specificity and accuracy
The sensitivity and specificity were obtained at various cut-off levels for each modality (plasma FSH, combined testicular volume, presence of testicular spermatids and Johnsen score) and the corresponding ROC plots are shown in Figure 1. The outcome of testicular extraction in relation to the presence of testicular spermatids is shown in Table II
. The sensitivity was 71%, specificity 92%, positive predictive value 95% and negative predictive value 58% for predicting the likelihood of successful testicular sperm extraction using the visualization of testicular spermatids on testicular histology as the predicting variable.
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Discussion |
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The poorer predictive values of testicular volume and plasma FSH concentration compared to the presence of testicular spermatids are not surprising. Variations in plasma FSH concentration can arise for reasons unrelated to spermatogenesis and many patients with maturation arrest have normal plasma FSH concentrations and testicular volume (Martin-du-Pan and Bischof, 1995). Testicular volume shows a wide variation as a result of many factors, including racial variation (Takihara et al., 1983). Although 79% of our patients were caucasians, 21% came from other ethnic backgrounds. The epididymis or scrotum can obscure the true measurement of testicular volume made with an orchidometer. Estimation of testicular volume using ultrasonography may improve its accuracy (Patel and Pareek, 1989
). Plasma FSH is directly under the influence of the Sertoli cell via inhibin and Leydig cells via negative feedback from testosterone. However, the activities of the germ cells themselves, rather than the Sertoli or Leydig cells, are assessed directly when the presence of spermatids is assessed. That spermatids can predict successful testicular sperm retrieval is not surprising, as maturation arrest is usually rare (Söderström and Suominen, 1980
; Silber and Rodriguez-Rigau, 1981
), apoptosis has decreased and cell division has ceased at the level of spermiogenesis, so that some testicular spermatozoa would invariably be produced somewhere in the testes once spermatogenesis has progressed to this stage.
To investigate the independent variables that are predictive of successful testicular sperm extraction, logistic regression analysis was undertaken to avoid the related effects of different co-variates. The model identified only two independent predictors (testicular spermatid and Johnsen score). The odds of successful sperm extraction if spermatids were present were 168 times higher than if they were absent having taken the Johnsen score into consideration. Although the 95% confidence intervals are very wide, there is a clinically important relationship between testicular spermatids and testicular sperm extraction because the true odds ratio is 6. The reason for the relatively low predictive value of the Johnsen score is unknown but may reflect the fact that the score was originally designed to correlate sperm count with testicular pathology in men with oligozoospermia. The improved performance of the Johnsen score in the logistic regression analysis compared to analysis with ROC may be due to the removal of the effects of other covariates associated with spermatogenesis. The diagnostic index correctly classified 87% of the patients in the derived sample. The misclassification observed was due to the focal nature of spermatogenesis with the result that the tissues taken for histology was different in terms of the status of spermatogenesis from those used for testicular sperm extraction (Tournaye et al., 1997
). However, the addition of Johnsen score to testicular spermatids improved its sensitivity, the negative predictive value and the accuracy of prediction (Tables II and VII
). The quality of the histopathological evaluation of diagnostic testicular biopsies is critical to the predictive power of the diagnostic index. A number of factors, including the method of handling of the biopsy sample, tissue fixation, embedding and sectioning, as well as the experience of the assessor are important (Holstein et al., 1994
). Seminiferous tubules are very delicate and must be collected by an atraumatic technique. Bouin's solution has been reported to be better than formalin for tissue fixation and epoxy resin better than paraffin wax for tissue embedding in terms of minimizing the distortion of tissue architecture (Holstein et al., 1994
). Despite the use of paraffin for tissue embedding, all our histological slides were suitable for analysis and a detailed cytological evaluation of the various stages of spermatogenesis was possible. The combined opinion of different assessors reduced the possibility of inter- and intra-observation errors.
Exploration of the genital tract was not used to exclude obstructive azoospermia and patients with primary gonadal failure were selected on clinical criteria. Testicular histology confirmed this diagnosis in all the patients studied. In spite of this we achieved a sperm retrieval rate of 70%, similar to the value of 62% reported by Schlegel et al. (1997) from a single large biopsy but higher than the value of 50% reported by Tournaye et al. (1996) from up to 20 small multiple biopsies. This finding suggests that the quantity of tissue used for sperm extraction may be as important as the site of the biopsy. The efficacy of sperm extraction may be further increased by the use of a combination of methods including in-vitro culture of testicular tissue, the use of erythrocyte lysing buffer and mechanical shredding of the testicular tissues (Zhu et al., 1995; Silber et al., 1996; Nagy et al., 1997
). Moreover, the testicular sperm retrieval rate also was not correlated with sperm vitality or with the outcome of ICSI in our study.
It is important to state the benefits and limitations of this diagnostic index. (i) Although the process of deriving the diagnostic index is complicated, it is very simple to use since it involves only two variablesspermatids and Johnsen score. Most pathologists can assess Johnsen score and the presence of spermatids from a diagnostic testicular biopsy and the natural logorithm values of the predicted scores can be calculated with or without a computer to obtain the predicted probability for each individual patient. (ii) Because more than one oocyte is often retrieved during ICSIIVF treatment, the single testicular spermatozoon used as the main outcome measure in this analysis is less than the 610 spermatozoa which are usually required for successful ICSI (Devroey et al., 1996). Nevertheless, the exact number of testicular spermatozoa which should be retrieved in a prior diagnostic biopsy before a couple proceeds to ICSI is unresolved. Although some patients are ready to undergo ICSI treatment in the presence of only a single testicular spermatozoon, this is unrealistic and has a poor prognosis. (iii) The predictive power of our diagnostic index contains a margin of error indicated by the confidence interval. Hence it is necessary to continue data collection in order to improve this. (iv) This model should ideally be validated prospectively in a larger cohort. However, it has been suggested that model testing in a derived population using the Jackknife technique is nearly as good as its evaluation in a prospective population (Efron and Gong, 1983). (v) It can be argued that a testicular biopsy must be obtained to provide the diagnostic index and hence that any spermatozoa obtained may be cryopreserved for future ICSI. Moreover, it has been demonstrated that both diagnostic and therapeutic testicular biopsies may be associated with potential vascular complications (Jarow, 1991
; Harrington et al., 1996
; Schlegel and Su, 1997
). Nevertheless, a prior diagnostic testicular biopsy may enable detection of carcinoma in situ (CIS) of the testis, which occurs in 13% of patients with severe male factor infertility (Skakkebaek, 1978
; Devroey, 1996
). Half (50%) of such cases will progress to testicular cancer (Nevero et al., 1996). Both CIS and seminoma of the testis have been reported by patients undergoing TESE (Devroey, 1996
; Nevero et al., 1996; Tournaye et al., 1996
). In addition, there is increasing evidence that a preliminary diagnostic testicular biopsy can ascertain that complete spermatogenesis is present in at least in one of the seminiferous tubules (Silber et al., 1997
; Tournaye et al., 1997
; Vanderzwalmen et al., 1997
). Percutaneous needle aspiration does not suffice for sperm extraction in men with azoospermia due to defective spermatogenesis (Frielder et al., 1996; Ezeh et al., 1998
), and a single needle biopsy may cause less vascular injury than a single open biopsy (Harrington et al., 1997). Only ~4 µg of testicular tissue is required for histopathological examination and the assessments of Johnsen score and testicular spermatids (Craft et al., 1997
) causing minimal vascular injury to the testis. (vi) It has recently been found that the outcome of oocyte injection with spermatids is better in patients with incomplete spermiogenic arrest (who have round, elongating or elongated spermatids) than in patients with complete spermiogenic arrest (no development beyond the round spermatid stage) (Vanderzwalmen et al., 1997
; Amer et al., 1998
). Since microspermatid injection of oocytes is currently banned in the UK, our therapeutic biopsies were not classified, but future studies should relate the stage of spermatid development to the outcome of microinjections. (vii) The presence of spermatids is a categorical variable with a binary outcome, and hence can only generate one reference point on the ROC plot. This type of analysis may be limited for a categorical variable without many reference points, relative to continuous variables such as plasma FSH, testicular volume, Johnsen score or a combination of categorical variables with multiple outcomes. However, a subsequent analysis with logistic regression analysis confirmed the value of this assessment campared with other variables in predicting the likelihood of successful testicular sperm extraction. Moreover, the area under the ROC curve was calculated from a MannWhitney test rather than the trapezoidal rule, which is more sensitive to the location and the spread of points defining the ROC curve (Hanley and McNeil, 1982
).
This study suggests that the presence of testicular spermatids and the Johnsen score as measures of germ cell status are the most reliable variables associated with successful testicular sperm extraction. Further research on the prediction of testicular sperm retrieval should therefore focus on germ cell assessment. The results also suggest that the predictive power is improved by the inclusion of the Johnsen score. These results provide further support for the use of a diagnostic testicular biopsy prior to a therapeutic testicular biopsy for testicular sperm extraction (Tournaye et al., 1996; Silber et al., 1997
; Vanderzwalmen et al., 1997
). To gain maximum information and optimize results for men undergoing an unpleasant invasive procedure, a combination of histology, trial testicular extraction and cryopreservation of testicular tissue or spermatozoa for use at a later date should be the procedure of choice.
Appendix 1
Determination of the area under the receiver operating characteristic (ROC) plot. The area under the ROC plot was determined statistically using the MannWhitney U-test (Hanley and McNeil, 1982; Zweig et al., 1993) using the formula:
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Appendix 2
The formulae for predicting testicular sperm retrieval using the presence of one testicular spermatid and the Johnsen score:
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Example of how to use the diagnostic index
(a) A patient with a Johnsen score of 7 and with spermatids seen on histology:
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Notes |
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References |
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Submitted on June 8, 1998; accepted on December 9, 1998.