1 Department of Obstetrics and Gynaecology, Centre for Reproductive Biology, University of Edinburgh, Edinburgh, 2 Shanghai Institute of Family Planning Technical Instruction, Shanghai, People's Republic of China, 3 Drug Control Centre, King's College London and 4 MRC Human Reproductive Sciences Unit, Centre for Reproductive Biology, 37 Chalmers Street, Edinburgh EH3 9ET, UK
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Abstract |
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Key words: desogestrel/male contraception/progestogen/spermatogenesis/testosterone
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Introduction |
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Progress towards an acceptable contraceptive formulation is also hampered by the characteristics of the testosterone preparations available. Ideally, testosterone should be administered as a zero-order release preparation with a long duration of action. Many studies investigating the hormonal approach to male contraception have used relatively short-acting injections of testosterone esters, administered weekly. Testosterone pellets are an alternative preparation, providing a closer approximation to the ideal and which have been shown to be effective both alone (Handelsman et al., 1992) and in combination with a progestogen (Handelsman et al., 1996
; Martin et al., 2000b
).
Therefore the major problem remains the need to suppress spermatogenesis completely, particularly in Caucasian populations, which appear relatively resistant compared with Chinese and Asian men (World Health Organization Task Force on Methods for the Regulation of Male Fertility, 1995; Handelsman et al., 1995
), without adversely affecting non-reproductive androgen-dependent processes. A third aspect is the acceptability to the user of the method: we have recently demonstrated that an oral preparation is more acceptable than an injectable form among a wide range of men surveyed (Martin et al., 2000a
).
We have recently investigated the dose-dependency of gonadotrophin suppression using the oral progestogen desogestrel with testosterone pellets (Martin et al., 2000b) and have demonstrated that repeated administration of testosterone pellets in such a regimen could maintain testosterone concentrations in the physiological range in normal men with significant suppression of spermatogenesis (Kinniburgh et al., 2001
). The primary objective of the present study was to investigate the degree of suppression of spermatogenesis, particularly the prevalence and maintenance of azoospermia with this regimen in two different ethnic populations.
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Materials and methods |
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Study design and medication
The study was a prospective randomized trial comparing two doses of oral desogestrel in combination with testosterone pellets, using the same treatment protocol in two centres and designed to have sufficient power to detect differences in the prevalence of azoospermia between the desogestrel doses. Subjects were randomized equally in each centre into two treatment groups to receive either 150 µg or 300 µg desogestrel (NV Organon, Oss, The Netherlands) p.o. daily for 24 weeks. Additionally all men received 400 mg testosterone pellets (2x200 mg; NV Organon) inserted s.c. under local anaesthetic into the anterior abdominal wall on the first day of desogestrel treatment. This was repeated 12 weeks later. All insertions in each centre were performed by the same investigator (D.K. and H.Z.) following appropriate training.
Subjects were reviewed and examined 2 weeks after commencing medication and subsequently at 4-week intervals during the treatment phase and the recovery phase of 16 weeks after finishing desogestrel. At each visit, subjects were examined and adverse events or other health problems recorded, semen samples produced and venepuncture performed. First-void urine specimens were collected from the Edinburgh cohort pretreatment, at 12 and 24 weeks treatment, and after 16 weeks recovery. Compliance was assessed by pill returns and direct questioning of subjects. Compliance in the Shanghai cohort was additionally assured for the first 12 weeks treatment by daily supervision by medical staff, which was discontinued thereafter by which time the reliability of the subjects had been established. Direct supervision was not possible in the Edinburgh cohort. Subjects were required to continue their current method of contraception.
After 24 weeks of treatment, subjects who were azoospermic at that time were given a choice of terminating treatment or continuing for a further 24 weeks. The aim of the extension phase was to investigate the maintenance of azoospermia over a longer time period. During this extension phase, subjects continued with oral desogestrel at the same dosage as they had taken up to that point and had further 400 mg testosterone pellets inserted at 24 weeks and 36 weeks of treatment. Clinical review, semen analysis and blood sampling continued at 4 weekly intervals throughout the extension phase. The men entering the extension phase were counselled regarding discontinuation of other forms of contraception for this phase of the study and, if they chose to do so, this was recorded. After completing a total of 48 weeks treatment these subjects entered the recovery phase. Non-azoospermic subjects and those choosing not to continue treatment commenced the 16 week recovery phase immediately after 24 weeks treatment.
Assays
Semen samples were submitted after 37 days abstinence. Each semen sample was assessed for sperm concentration using WHO methodology (World Health Organization, 1992). Azoospermia was confirmed by examination of the pellet following centrifugation of the ejaculate; samples with sperm seen only in the pellet were given a nominal concentration of the limit of quantification (1x106/ml).
Blood samples were obtained between 0700 and 1200 h at every visit. Every 12 weeks, blood was analysed for haematological and biochemical values by routine autoanalyser. Further samples were separated by centrifugation and serum stored at 20°C until assay. Hormone assays in Edinburgh were performed as previously described (Anderson and Wu, 1996): testosterone was measured by radioimmunoassay (Corker and Davidson, 1978
), and LH and FSH by time-resolved immunofluorometric assay (Delfia, Wallac, Turku, Finland). Assay sensitivities were 0.15 and 0.125 IU/l for LH and FSH respectively, and coefficients of variation (CV) were 6.9% (testosterone), 8.3% (LH) and 9.8% (FSH). Samples from individual subjects were measured in the same assay. Etonogestrel, the active metabolite of desogestrel, was measured by in-house radioimmunoassay (Organon NV). In Shanghai, testosterone was measured by radioimmunoassay (Spectria; Orion Diagnostica, Espoo, Finland) as were LH and FSH (Sino-American Joint Venture Tianjin Jiuding Medical Bioengineering Ltd, China): gonadotrophin assay sensitivities were 1.2 IU/l, assay CV were 5.8% (testosterone), 6.7% (LH) and 7.3% (FSH).
Other serum and urinary analytes were measured in Edinburgh only. Sex hormone binding globulin (SHBG) was measured by immunoradiometric assay (DPC, Los Angeles, CA, USA). Urinary epitestosterone (EpiT) was measured by capillary column gas chromatographymass spectrophotometry (GC-MS) as previously described (Cowan et al., 1991; Kicman et al., 1999
). Inhibin B and inhibin forms containing pro and
C (pro-
C) immunoreactivity were measured as previously described (Groome et al., 1995
, 1996
). Assay sensitivities for inhibin B and pro-
C were 15 and 3 pg/ml respectively, and CV 15% (inhibin B) and 7.3% (pro-
C).
Serum concentrations of both total and bone-specific alkaline phosphatase (T-ALP and B-ALP) were assayed. T-ALP was measured as previously described (Committee on Enzymes of the Scandinavian Society for Clinical Chemistry and Clinical Physiology, 1974). B-ALP was measured by electrophoresis in agarose gel following brief incubation with neuraminidase (Helena Laboratories, Sunderland, UK). Pyridinium cross-links were measured in urine by isocratic reverse phase high performance liquid chromatography (Pratt et al., 1992
; Crofton et al., 1998
). Deoxypyridinoline and pyridinoline results were expressed in relation to creatinine measured on the same urine sample.
Behavioural assessment
Sexual interest and activity were investigated pretreatment and at 12 week intervals during treatment and recovery. A structured interview was used to quantify sexual activity over the preceding 2 weeks in both centres, and the Frenken Sexual Experience Scales (SES) 2 and 3 were used to provide measures of psychosexual arousability and sexual motivation (Martin et al., 2000b; Anderson et al., 1992
). As these scales were developed for a Caucasian population and no adequate translation or controls exist for Chinese men, the Frenken scales were not performed in Shanghai. Subjects' partners also returned a simple questionnaire at the same time points, which recorded any perceived changes in subject's mood, sexual interest and sexual activity (Martin et al., 2000b
).
Data analysis
Results are presented as mean ± SEM. Hormonal data were log-transformed to correct non-equality of variance before analysis of variance (ANOVA) and sperm concentrations were cube root-transformed prior to ANOVA. Paired t-tests were used to investigate at what time points a significant treatment effect was seen for each group. Analysis of co-variance (ANCOVA) was used to test for an effect of dose after adjusting for baseline values if appropriate. Categorical data were analysed by Fisher's exact test.
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Results |
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Minor adverse events included mood changes (n = 9), weight gain or increased appetite (n = 5), acne (n = 4), libido change with both decreases (n = 4) and increases (n = 3) reported, and implant-related (n = 8) which included two pellet expulsions, both in the Shanghai cohort. Other implant problems were of discomfort, with no episodes of haemorrhage or infection. There were no testosterone pellet expulsions in the Edinburgh cohort. Most minor side effects were of <1 week duration. Experience of minor side-effects was comparable between the two treatment groups. Subjects in both Edinburgh and Shanghai gained weight during the study. Median change was +0.3 kg (range 6 to +7.3 kg) in Edinburgh and +2.5 kg (range 4 to +11.5 kg) in Shanghai. There was no significant difference in weight gained between the two treatment groups in either centre.
Fifteen men entered the extension phase of the study, four men in the 150 µg group and four men in the 300 µg group in Edinburgh, and six from the 150 µg group and one from the 300 µg group in Shanghai. Two men withdrew for personal reasons after 36 weeks treatment (both in the 150 µg group in Edinburgh), the remaining 13 subjects completed 48 weeks treatment in total.
Compliance with oral desogestrel use was high. In the 150 µg group in Edinburgh, subjects reported missing a median of one dose (range 05), and a median of 1.5 doses (range 011) in the 300 µg group. Numbers of pills which were returned confirmed high levels of self-reported compliance with medication with only 1.1% of pills being returned with the exception of one subject in the 300 µg group who returned medication amounting to 14 doses at week 20, and who admitted to a period of poor compliance between weeks 16 and 20. Etonogestrel concentrations measured in the Edinburgh centre after 20 weeks treatment were 771 ± 119 pg/ml in the 150 µg group and 1282 ± 192 pg/ml in the 300 µg group. Compliance in the Shanghai cohort was assured for the first 12 weeks treatment by daily supervision by medical staff. Thereafter medication was administered as in Edinburgh, and no individuals reported non-compliance of more than two doses.
Sperm concentration
Both treatment groups showed a rapid and profound suppression of sperm concentration (P < 0.001, Figure 1). This reduction was comparable in both ethnic groups. In particular, all men in the 300 µg group (n = 28, 95% CI 86.3100.0%) from both ethnic groups achieved azoospermia at some stage within 16 weeks of commencing treatment. While there were no significant differences in sperm concentrations between the two treatment groups within either centre at any time point, the proportion of men in Edinburgh with sperm concentrations <3 x106/ml was significantly greater in the higher dose group at 8 weeks (13/14 versus 8/15, P = 0.03). Thus there was some evidence that suppression was faster in the 300 µg group than in the 150 µg group in Edinburgh but not in Shanghai. Figure 2
shows the number of subjects with sperm concentrations of <3x106/ml, <1x106/ml and azoospermia during treatment in both centres. Within 12 weeks of treatment, azoospermia was achieved in 23/28 (82.1%) of the 300 µg group and 17/32 (53.1%) of the 150 µg group. There was a significant difference between the two treatment groups in the number of subjects achieving azoospermia (P < 0.05). In the 150 µg group, 22/31 men achieved azoospermia (71.0%: 95% CI 52.085.8%) during the course of the study, 11/13 (84.6%) in the Caucasian group and 11/18 (61.1%) in the Chinese group, all 22 men submitting azoospermic semen samples on at least two occasions. In the 150 µg group, all Caucasian men achieved sperm concentrations of <1x106/ml at some stage during treatment whereas three Chinese men consistently maintained sperm concentrations >3x106/ml. While non-compliance was possible during the second 12 weeks of treatment in these three individuals, all complied fully during the first, supervised, 12 weeks. Sperm concentration remained in the normal range in these men at 12 weeks, whereas it was suppressed to <7x106/ml in all other men. Only one man in the Edinburgh cohort showed temporary recovery from azoospermia during treatment, the subject who admitted very poor compliance between weeks 16 and 20. His sperm concentration was 6.2x106/ml at 20 weeks, and following improved compliance he was again azoospermic at 24 weeks. Similar results were seen in two men in the 300 µg group in Shanghai, who had sperm concentrations of 4 and 7x106/ml at single time points having been azoospermic 4 weeks previously, and who became azoospermic again thereafter.
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Testosterone
Testosterone concentrations remained within the physiological range throughout the treatment period in all groups (Figure 3). There was an initial fall at the start of treatment (P < 0.001 at 4 weeks versus pretreatment), with a later rise at 16 weeks following administration of the second testosterone dose at 12 weeks. Those subjects who entered the extension phase had similar testosterone concentrations in the second 24 weeks of treatment, with transient peaks at 28 and 40 weeks treatment following further testosterone placements.
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Sex hormone binding globulin (SHBG)
SHBG concentrations in the Edinburgh cohort fell significantly during treatment (P = 0.042, 150 µg group; P = 0.001, 300 µg group) followed by recovery to initial levels 16 weeks after end of treatment (Figure 5). There was no significant difference in SHBG concentrations between the two groups. Despite the fall in SHBG, calculated free testosterone concentrations (Vermeulen et al., 1999
) at 12 and 24 weeks treatment were significantly lower than pretreatment concentrations in both groups (P < 0.003, 150 µg group; P < 0.001, 300 µg group; Figure 5
). There were no differences between groups and free testosterone concentrations at 16 weeks recovery were not significantly different from pretreatment.
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Inhibin
Concentrations of pro-C inhibin forms and dimeric inhibin B in both serum and seminal plasma for the Edinburgh centre are shown in Figure 6
. Inhibin B in peripheral blood showed no significant changes over the study period in either group. Pro-
C inhibin fell markedly during treatment with concentrations dropping to approximately half of pretreatment levels after 4 weeks treatment in both groups (P < 0.001) with no differences between groups, which was maintained for the duration of treatment. There was a prompt recovery of pro-
C concentrations on discontinuing desogestrel. Seminal plasma inhibin B concentrations showed a marked variation between individual subjects, ranging from 45pg/ml to 10500pg/ml pretreatment. During treatment, concentrations fell (P = 0.018, 150 µg group; P = 0.011, 300 µg group). Although inhibin B concentrations in seminal plasma did not differ statistically between the groups during treatment, it became undetectable in 7/13 men in the 150 µg group and 10/12 men in the 300 µg group by 24 weeks: samples were not available for assay from the remaining men.
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Bone markers
Both treatment groups (150 and 300 µg desogestrel) showed similar significant reductions in the concentrations of both total and bone-specific alkaline phosphatase concentrations and have therefore been analysed together (T-ALP, P < 0.001; B-ALP P = 0.006; Table I). These falls were evident by 4 weeks of treatment and recovered to pretreatment levels following discontinuation of desogestrel. Urinary deoxypyridinoline and pyridinoline concentrations were unchanged throughout the study period (Table I
).
Behavioural assessments
There were no differences in frequency of sexual intercourse or SES scores between the two treatment groups pretreatment or during treatment, and the results are therefore presented grouped together for each centre (Table II). There was no significant change in the reported frequency of sexual intercourse or in the behavioural questionnaires during the the study.
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Discussion |
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The administration of injectable testosterone esters results in azoospermia in approximately two-thirds of Caucasian men, and in a greater proportion of Chinese men (World Health Organization Task Force on Methods for the Regulation of Male Fertility, 1995). The co-administration of a progestogen allows the dose of testosterone to be reduced (Foegh et al., 1980
; Bebb et al., 1996
; Handelsman et al., 1996
; Meriggiola and Bremner, 1997
). It remains necessary to avoid the supraphysiological concentrations resulting from the currently available testosterone esters (Behre and Nieschlag, 1998
) and to increase the prevalence of azoospermia particularly in Caucasian populations. Testosterone pellets provide reproducible plasma testosterone concentrations without the need for frequent administration (Handelsman, 1998
) and our preliminary data using testosterone pellets with oral desogestrel suggested that this combination might result in the necessary degree of suppression of spermatogenesis (Martin et al., 2000b
). The main limitations of testosterone pellets are the need for a minor procedure for insertion, and occasional pellet extrusion. Extrusion was infrequent in the present study, within the range reported by other experienced groups (Handelsman, 1998
). While it is likely that long-acting injectable testosterone preparations (Nieschlag et al., 1999
; Zhang et al., 1999
) may be preferred, testosterone pellets are a valuable prototype of a depot preparation.
The ethnic variability in response to hormonal male contraceptive regimens indicates the importance of studying such regimens in different populations. However, we found no marked differences in the degree of suppression of spermatogenesis between Caucasian and Chinese recruits with the present regimen. Indeed, surprisingly, there was a trend towards greater suppression in the Caucasian cohort in the 150 µg group. A testosterone-only group was not included in the design: the same low dose of testosterone pellets administered alone to Caucasian men has been clearly shown to have no suppressive effect on spermatogenesis (Handelsman et al., 2000). However, it should be noted that the additive effect of progestogen has not been clearly demonstrated in Asian men and was not assessed in the present study. Although differences in drug compliance between centres cannot be excluded as the basis for non-suppression in some Chinese men, these data imply that the differences previously obtained with testosterone-only administration (World Health Organization Task Force on Methods for the Regulation of Male Fertility, 1995
) may not be a characteristic of all contraceptive regimens. While there appear to be ethnic differences in susceptibility to spermatogenic suppression, some regimens such as that investigated here might override any such differences.
Gonadotrophin secretion was profoundly suppressed during treatment, to consistently undetectable concentrations in the 300 µg group. Suppression was less complete in the 150 µg group, although both LH and FSH were undetectable in most men in most samples. There were, however, significant fluctuations in both LH and FSH in the 150 µg group with a reciprocal relationship to changes in testosterone concentrations. Thus gonadotrophins tended to rise, i.e. escape from suppression, at 12 and 24 weeks at the nadir of testosterone concentrations. These data illustrate the dose-dependency of gonadotrophin suppression by desogestrel, but also clearly show the significant contribution of fluctuations in testosterone concentrations, revealed with the sub-maximal desogestrel dose. These data show that at this threshold dose of testosterone the undetectable decrease in testosterone absorption in the third compared with the first month following insertion (Handelsman, 1998) is of considerable importance to the degree of gonadotrophin suppression achieved. Gonadotrophin concentrations were less completely suppressed in those men who maintained detectable sperm concentrations. It is thus probable that, with a slightly higher testosterone dose, suppression of spermatogenesis in the 150 µg group would have been more complete. Etonogestrel concentrations were comparable with those reported elsewhere with the same doses (Wu et al., 1999
; Anawalt et al., 2000
) and subjects maintaining spermatogenesis had similar concentrations to azoospermic subjects. In Shanghai, desogestrel was administered by study personnel daily for the first 12 weeks of treatment. Although it is possible that non-compliance thereafter contributed to the lack of suppression to azoospermia in some men, these men showed significant sperm concentrations throughout the treatment period. It is therefore more likely that the incomplete suppression of spermatogenesis in the group receiving 150 µg desogestrel is due to the dose being insufficient for maximal effect. Testosterone pellet extrusion did not contribute to lack of efficacy, as it did not occur in any men in the 150 µg group.
Measurement of other testicular products may provide insight into the mechanism of suppression of spermatogenesis by this regime. Inhibin B is a product of Sertoli cells and has a role in pituitary feedback on gonadotrophin secretion (Anderson and Sharpe, 2000). Evidence from some contraceptive studies has demonstrated a fall in inhibin B with suppression of spermatogenesis (Anawalt et al., 1996
; Anderson et al., 1997b
). However, in this study, serum concentrations were maintained despite azoospermia being achieved in all subjects in the 300 µg desogestrel group, consistent with our previous finding in a short-duration study of this regime (Martin et al., 2000b
). In contrast, pro-
C concentrations fell rapidly during treatment, indicating the closer relationship between gonadotrophins and pro-
C secretion. Seminal plasma also contains dimeric inhibin B. The significant correlation between seminal inhibin B concentration and sperm concentration suggests that it may reflect the activity of the seminiferous epithelium (Anderson et al., 1998
). A marked reduction in the concentration of seminal plasma inhibin B in those subjects who became azoospermic (Martin et al., 2000b
) was confirmed with the current study. The disparity between systemic and seminal inhibin B concentrations demonstrates that serum inhibin B concentration is a poor marker of spermatogenesis in this context. The basis for the lack of change in serum inhibin B during gonadotrophin suppression is unclear. It is possible that this reflects the loss of integrity of inter-Sertoli-cell junctions (Kerr, 1991
; Muffly et al., 1994
), preventing the normal regulation of the bi-directional secretion of inhibin B and allowing the high concentrations within the seminiferous tubule access to the interstitial space.
EpiT is a natural epimer of testosterone secreted predominantly by the testis (Dehennin and Matsumoto, 1993; Kicman et al., 1999
). In our previous study in which 24 h urine collections were analysed, EpiT excretion fell to a similar degree to that reported here (Anderson et al., 1997a
). This is compatible with the 90% reduction in directly measured intratesticular testosterone levels reported in normal men treated with testosterone propionate (Morse et al., 1973
). EpiT remained detectable in all men during treatment with concentrations severalfold higher than found in hypogonadal men (Kicman et al., 1999
). It therefore appears that there remains a low rate of steroidogenesis within the testis during desogestrel/testosterone treatment. The small number of men who did not become azoospermic precludes statistical analysis of EpiT excretion by that variable, but there was no evidence of higher excretion in those men during treatment.
The completeness of suppression of spermatogenesis with the present regimen precludes comparison of responders with non-responders, but comparison may be made with other regimens. A major difference may lie in the characteristics of the testosterone preparation used. In a previous study using oral desogestrel with injectable testosterone enanthate, it appeared that azoospermia was more prevalent in groups receiving lower doses of testosterone (Wu et al., 1999) although group sizes were small. The high prevalence of azoospermia in the present study may therefore be related to the avoidance of high post-injection testosterone concentrations, as well as the relatively low levels of testosterone overall. The dose of testosterone administered here (400 mg/12 weeks) was calculated to explore the possibility of replacement in the low physiological range, in contrast to the majority of studies in this field which have used higher doses of testosterone. Direct determination of testosterone absorption from these pellets indicates that this dose will release 2.6 mg testosterone per day (Handelsman, 1998
), i.e. lower than most estimates of normal production rates (Southren et al., 1968
; Vierhapper et al., 1997
; Santner et al., 1998
). Consistent with this, there was an initial fall in total testosterone concentrations, to
60% of the pretreatment concentration at 12 weeks. However, as testosterone pellets are fully bioavailable over
6 months (Handelsman, 1998
), repeated administration as in the present protocol involving administration on up to four occasions should mean that the steady-state dose release is
4.8 mg/day. In fact, testosterone concentrations in those men who received treatment for 48 weeks continued to show very similar fluctuations to that seen in the first 24 weeks. It should, however, be noted that pretreatment samples were taken in the morning, and thus may be higher than the 24 h average in healthy young men (Plymate et al., 1989
).
It is important to consider whether maintenance of testosterone concentrations in the low physiological range as here might have adverse or beneficial effects on other reproductive and non-reproductive androgen-sensitive target organs including liver metabolism, haematopoiesis, muscle and bone. Regimens involving administration of testosterone alone or with progestogens to men generally result in potentially adverse changes in lipoproteins, particularly HDL-C (Wallace and Wu, 1990; Bebb et al., 1996
; Wu et al., 1996
). A decrease in HDL-C and total cholesterol were seen in the Edinburgh but not Shanghai cohorts. Similarly Chinese men did not show changes in lipoprotein concentrations during testosterone-only treatment (Wu et al., 1996
). Haematological parameters may be a sensitive index of androgen dosage, and no changes were detected. This contrasts with the increase in haemoglobin and haematocrit observed during supraphysiological testosterone treatment (Wu et al., 1996
), and the decrease during testosterone/cyproterone acetate treatment (Meriggiola et al., 1996
). Muscle mass and strength were not addressed in this study but possible long-term effects are clearly important. Changes in bone metabolism may also be of value in the investigation of the non-reproductive effects of contraceptive steroid administration as an index of androgenicity. Few studies of male contraceptive steroid regimens have investigated possible effects on bone turnover. One study using testosterone alone showed increases in biochemical markers of bone turnover and also in bone mineral density (Young et al., 1993
). Hypogonadism in men results in increased B-ALP activity and urinary excretion of pyrodinoline and deoxypyridinolone, indicating increases in both bone formation and resorption, and conversely testosterone treatment of hypogonadal men reduces these parameters and results in increased bone mineral density (Anderson et al., 1997
). In the present study bone alkaline phosphatase was reduced, while urinary deoxypyridinoline and pyridinoline were unchanged. A fall in total alkaline phosphatase has recently been reported in a study in normal men involving administration of a larger dose of testosterone with norethisterone (Kamischke et al., 2001
). Changes in these biochemical markers cannot be readily extrapolated to indicate changes in bone mineral density, and the clinical relevance of these findings is uncertain. Biochemical assessment of the adequacy of testosterone replacement other than by testosterone concentrations is uncertain (Bhasin et al., 1998
), yet is becoming increasingly important as both contraceptive regimens and hypogonadal replacement therapies may include androgens other than testosterone itself (Sundaram et al., 1993
; Anderson et al., 1999
; Negro-Vilar, 1999
).
In conclusion, oral desogestrel with a long-acting testosterone preparation results in a very high prevalence of azoospermia, which can be maintained for a prolonged period of time. The need for improved testosterone preparations remains, but these data indicate that this is a promising approach to the development of a valid method that will meet the requirements of men and women (Glasier et al., 2000; Martin et al., 2000a
).
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Acknowledgements |
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Notes |
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Submitted on December 18, 2001; accepted on February 8, 2002.