In Vitro Fertilization Unit, Department of Obstetrics and Gynecology, Hadassah University Hospital, Ein Kerem, PO Box 12000, Jerusalem 91120, Israel
1 To whom correspondence should be addressed. e-mail: Revel{at}md.huji.ac.il
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Abstract |
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Key words: assisted reproductive techniques/cryopreservation/frozen embryo/IVF/multiple pregnancy
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Introduction |
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The length of time that an embryo can remain frozen, and be successfully transferred, remains unknown.
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Case report |
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As a result of the fresh IVF cycle performed in 1990, eight embryos (410 cells grade A, B) were cryopreserved 72 h after fertilization using 1,2-propandiol (PROH) as the cryoprotectant (Testart et al., 1987). Briefly, the embryos were washed in enriched phosphate-buffered saline (PBS) medium containing 20% maternal serum and 0.4% human serum albumin (Sigma, St Loius, MO, USA) before being transferred to the same medium also containing 1.5 mol/l PROH (Sigma). Following 15 min of incubation, the embryos were transferred to an ampoule containing 250 µl of enriched PBS medium containing 1.5 mol/l PROH and 0.1 mol/l sucrose. Freezing protocol included slow cooling at a rate of 20°C/min to 7°C, at which temperature manual seeding was performed. The embryos were then cooled at a rate of 0.3°C/min to 30°C, at which temperature the ampoule was plunged into liquid nitrogen (LN2) for long-term storage. Our embryo bank consists of several TaylorWharton LN2 containers (Theodore, AL) and the LN2 level is continuously monitored and attached to an alarm system (Custom Biogenic Systems, Shelley Township, MI).
At the couples request, and after written consent, being aware of the fact that cryopreservation technique was suboptimal 12 years ago, it was decided to transfer four embryos. Embryos were thawed using commercially available solutions (Irvine Scientific, Santa Ana, CA, USA). The embryos were incubated in a series of solutions containing decreasing PROH concentrations before being transferred to culture medium (Testart et al., 1987). Embryos were maintained in culture until their transfer within 2 h. Full blastomere survival was noted in all four embryos.
The four thawed embryos were transferred using artificial endometrial preparation. This include daily oral micronized E2 tablets from day 1 of the cycle for 2 weeks, followed by concomitant administration of micronized progesterone placed in the vagina as reported previously (Simon et al., 1999). After 3 days of progesterone exposure, embryo transfer was performed. The embryos were transferred using a Casmed (Casmed International, Banstead, UK) catheter under sonographic guidance.
A triplet pregnancy resulted. Selective fetal reduction to twins was successfully performed at 13 weeks. During pregnancy the patient was treated with -methasone 12 mg from the 30th week. Caesarean section at 36 weeks resulted in the delivery of healthy twins weighing 2500 g each.
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Discussion |
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Good prognostic factors for the outcome of frozenthawed cycles include age <40 years, a pregnancy having resulted from the initial embryo transfers, and the embryo grade and cleavage stage at the time of freezing (Karlstrom et al., 1997). Implantation potential of frozenthawed human embryos with full blastomere survival has been shown to be 3-fold higher than partially damaged embryos (Van den Abbeel et al., 1997
) Moreover, the quality of the embryos was found to be more important than the duration of the storage as predictor of implantation (Kondo et al., 1996
; Salat-Baroux et al., 1996
). No increase in congenital malformations was seen following frozen embryo transfer (Salat-Baroux et al., 1996
; Wennerholm et al., 1997
; Aytoz et al., 1999
). Post-thaw culture may allow selection of the embryos that continue to cleave in vitro, and thus improve implantation rate per transferred embryo (Van der Elst et al., 1997
; Ziebe et al., 1998
). Thus, although embryo cryopreservation may adversely affects embryo quality, it does not seem to have detrimental effects on the implantation or pregnancy potential of high-quality thawed embryos (Selick et al., 1995
).
The policy at our IVF unit is to store cryopreserved embryos for as long as requested by the couple. This report, of the longest cryopreservation period to be followed by successful pregnancy and delivery, confirms the finding that the duration of the storage does not appear to adversely affect the survival of frozen embryos.
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References |
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Submitted on July 22, 2003; accepted on September 29, 2003.