Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, College of Physicians & Surgeons, Columbia University, New York, New York, USA
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Abstract |
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Key words: embryo/estradiol/oocyte/oocyte donation
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Introduction |
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For lack of a natural in-vivo human model, IVFembryo transfer cycles have been used as an alternative way to study the effects of peak E2 on embryo quality and endometrial receptivity. Some investigators have noted no adverse effects, while others demonstrated evidence of significant decreases in fertilization, implantation and pregnancy rates (Forman et al., 1988; Pellicer et al., 1989
, 1996
; Chenette et al., 1990
; Toner et al., 1991
; Simón et al., 1995
, 1998
; Sharara and McClamrock, 1999
; Ng et al., 2000
). Adverse effects of a supraphysiological E2 may include alterations in both endometrial receptivity and oocyte/embryo quality.
Unfortunately, observations related to conventional IVFembryo transfer may lead to erroneous conclusions, as heterogeneous diagnoses are present in this population of infertile patients. Confounding variables such as the presence of hydrosalpinges and severe endometriosis make it difficult to study individual effects related to oocyte/embryo quality and endometrial receptivity. Oocyte donation, on the other hand, represents a more ideal model because the oocyte donor's gametogenesis and ovarian steroidogenesis are dissociated from the recipient's endometrial development and receptivity. To date, only one group (Simón et al., 1995) has examined the effects of elevated peak E2 levels in oocyte donation cycles. However, interpretation of these data was difficult because the recipients received supernumerary oocytes from high responder (defined as
15 retrieved oocytes) infertile patients who themselves were undergoing IVFembryo transfer. In contrast to Simón et al. (1995), in the present study an oocyte donation model was chosen in which each recipient received all the oocytes retrieved from one designated donor. These donors were typically young (2134 years of age), healthy and not infertile, and represented a more homogeneous cohort than infertile populations. Similarly, the endometrial receptivity of the recipient was relatively constant because of uniform artificial preparation. In this way, it was possible to evaluate the consequences of peak E2 on oocyte/embryo quality independent of the effects on endometrial receptivity. The purpose of this study was to define these independent factors using the oocyte donation model.
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Materials and methods |
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The characteristics of the recipients were as follows [mean ± SEM (range)]: age 43.5 ± 0.3 (2656) years; gravidity 1.42 ± 0.13 (010); parity 0.40 ± 0.06 (04); male factor 50.6%. The characteristics of the donors were: age 25.9 ± 0.2 (2034) years; gravidity 0.64 ± 0.06 (06); parity 0.30 ± 0.04 (04).
Oocyte donors and recipients followed a standard synchronization regimen that was previously described (Sauer et al., 1989, 1992
) and did not vary throughout the study period. Briefly, all donors underwent ovarian down-regulation using leuprolide acetate. Once down-regulation was confirmed, leuprolide acetate was decreased and gonadotrophin (FSH or HMG) was started at a dose of two to four ampoules per day. The ovarian response was assessed by ultrasound and serum E2 level on the fifth day of gonadotrophin, and serially thereafter. HCG (10000 IU) was administered intramuscularly when lead follicles reached 1920 mm in diameter. A serum E2 (peak E2) level was obtained from all oocyte donors on the morning of HCG administration. Ultrasound-guided transvaginal oocyte retrieval was performed 3436 h later under intravenous conscious sedation. All visible follicles, regardless of diameter, were aspirated from both ovaries. At the time of retrieval, all recovered oocytes were graded for maturity (grade 2 oocyte with a light fluffy cloudy appearance and a radiating corona radiata). Oocytes were either inseminated or injected (ICSI) depending on sperm quality. Semen criteria for ICSI in the study included the following: sperm concentration <20x106/ml or <50% motility or <4% normal forms by Kruger strict criteria. Only embryos with two visualized pronuclei (2PN) were considered normally fertilized.
Recipients were synchronized using oral micronized E2 approximately 5 days before the donor-initiated gonadotrophin therapy. Recipients with gonadal function (n = 155; 57%) underwent leuprolide acetate down-regulation before starting E2. Progesterone was started on the morning of the day before the donor's retrieval and continued twice daily thereafter. All embryo transfers were performed 72 h post-retrieval. Before embryo transfer, embryos were graded according to a scale of 1 to 5 based on modified criteria established previously (Veeck, 1991). A grade 5 embryo would be optimal (embryo stage appropriate for time with equal size blastomeres, clear cytoplasm and no cytoplasmic fragments), while grade 1 embryo would be very poor quality (embryo stage not appropriate for time, irregular blastomeres with poor quality cytoplasm and significant fragmentation). To assess overall quality of the transferred embryo, an embryo score was calculated by multiplying the rating of the embryo by the number of its blastomeres. The scores of all embryos transferred per patient were summed and then divided by the number of embryos transferred to give the average embryo score (AES). Excess embryos of high-quality (grade 45) were then cryopreserved using a programmable freezer.
Following embryo transfer (n = 330), patients continued with supplemental E2 and progesterone until a negative pregnancy test was documented 12 days later or when pregnant until 14 weeks of estimated gestational age. Clinical pregnancy was defined as the presence of a gestational sac on ultrasound. Ongoing pregnancy was defined as a continuing pregnancy after 20 weeks gestation. Abortions were defined as losses prior to 20 weeks gestation. Peak E2 levels were grouped by 33rd percentile (group I <1500 pg/ml; group II 15003000 pg/ml; and group III >3000 pg/ml).
In order to examine the effects of E2 on the developmental capacity of oocytes and embryos, the following outcome measures were compared: number of oocytes retrieved; ratio of grade 2 oocytes to total oocytes retrieved; fertilization rates; number of embryos cryopreserved; number of embryos transferred; and cumulative embryo score. In addition, clinical outcomes were also studied including clinical pregnancy, ongoing/delivered pregnancy rate per embryo transfer and implantation rates.
Serum levels of E2 were measured by chemiluminescent enzyme immunoassays (Immulite, Diagnostic Products Corporation, Los Angeles, CA, USA). Intra-assay and inter-assay coefficients of variation did not exceed 9.3 and 10.5% respectively. Statistical analyses were performed using SPSS-PC (SPSS Inc., Chicago, IL, USA). Statistical differences between groups were determined by analysis of variance (ANOVA), t-test and 2 test. Correlations were analysed using the Pearson product moment correlation. All data were expressed as mean ± SEM unless otherwise noted. A P-value < 0.05 was considered significant.
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Results |
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Comparisons between groups revealed no differences in recipient age, or in the proportion of male factor patients that required ICSI (Table I). With regard to donor profile, significant differences in age, gravidity and parity (P < 0.05) were noted between groups, although there were no differences in baseline day 3 FSH and E2 levels. Patients in group III required the lowest number of ampoules of gonadotrophin for ovarian stimulation, but had the highest number of oocytes retrieved, number of 2PN, number of embryos transferred and cryopreserved (Table I
). Pearson correlations revealed that donors with higher peak E2 levels used fewer ampoules of gonadotrophin (r = 0.21, P < 0.005) and required fewer days of stimulation (r = 0.18, P < 0.005). There were no significant differences in oocyte quality, since the fertilization rate and the ratio of grade 2 oocytes to total oocytes were unchanged (Table I
). On the other hand, the AES of embryos transferred was significantly higher in groups II and III than in group I (P < 0.05) (Table I
). A direct correlation was noted between peak E2 level and AES (r = 0.213, P < 0.005). The number of embryos cryopreserved was also significantly higher in group II than group I, with group III numbers significantly higher than both of the other groups.
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Discussion |
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As stated earlier, data from previous studies using IVFembryo transfer as a model to examine the effects of high peak E2 on endometrial receptivity are difficult to interpret. Although evidence of an adverse effect on endometrial morphology exists, the clinical influence of high steroid concentrations remains largely unknown. When reviewing studies in which endometrial biopsies were performed during the `implantation window' of ovarian stimulation cycles, abnormal dating was demonstrated in as many as 73% of specimens (Cittadini and Palermo, 1991). Benadiva and Metzger found a higher incidence of dyssynchrony between endometrial glands and stroma following ovarian stimulation when compared with natural cycles (Benadiva and Metzger, 1994
). Similarly, others have reported impaired development of endometrial glands (Bonhoff et al., 1990
), advanced endometrial histological maturity (Garcia et al., 1984
), stromal development (Noci et al., 1997
) and an earlier expression of pinopodes (Martel et al., 1987
; Kolb et al., 1997
) following ovarian stimulation. In contrast, others (Hadi et al., 1994
; Macrow et al., 1994
) failed to find any detectable morphological changes in the endometrium. However, the former of these reports noted a significant reduction in nuclear receptors for progesterone and estrogen in both glands and stroma after ovarian stimulation. These endometrial responses to ovarian stimulation may be variable, but any change in endometrial morphology and thus the window of receptivity may adversely affect implantation. The composite effects of these hormonal alterations on endometrial receptivity require further exploration.
Oocyte and embryo quality may also be influenced by serum E2 secretion (Jones et al., 1983; Dor et al., 1986
). Previous studies have reported decreased fertilization rates with higher peak E2 levels (Pellicer et al., 1989
; Gelety and Buyalos, 1995
). More indirect evidence reveals that peak E2 levels in the fresh IVFembryo transfer cycles are not related to the success of frozenthawed embryo transfer cycles (Toner et al., 1991
; Schalkoff et al., 1993
; Ng et al., 2000
). Embryo quality appeared unaffected, as excess embryos from different groups of peak E2 levels had similar implantation and pregnancy rates during the freezethaw embryo transfer cycles. Nonetheless, a strong bias may have been present since only patients who did not conceive in the fresh cycle subsequently underwent a frozen embryo transfer cycle.
Oocyte donation is a more appropriate model to study the different compartments. The recipient's endometrial receptivity is dissociated from folliculogenesis since it is artificially prepared to be more uniform and similar to that of a natural menstrual cycle. Using a mouse embryo donation model, a reduced implantation rate was demonstrated in animals which had higher E2 levels following ovarian stimulation (Fossum et al., 1989). Data on human oocyte/embryo donation are generally lacking, however. In one report of data from oocyte donation (Simón et al., 1995
), the effects of high peak E2 levels in 108 cycles of 96 high-responder oocyte donations did not result in significant differences in fertilization, implantation and pregnancy rates. However, as stated previously, infertile women undergoing IVFembryo transfer who donate their supernumerary oocytes may present with confounding variables. Although not reported, these patients may have other diagnoses that might affect their oocyte and subsequent embryo quality, and their donated oocytes may be of poorer quality because the patient herself would first receive the oocytes of higher grade. Nevertheless, these authors concluded that oocyte and embryo quality was not affected by high levels of E2. Using the model described herein, in which each recipient received all the oocytes from one single designated donor who was healthy and not infertile, such biases were avoided. In accordance with a previous report (Simón et al., 1995
), the present study found that the high peak E2 levels were not associated with a lower ratio of high-grade oocytes, fertilization rates or embryo quality. These data suggest that the developmental quality of oocytes and embryos obtained was not impaired by elevated E2. On the contrary, higher levels correlated directly with a greater number of oocytes retrieved, more embryos available for transfer and cryopreservation, and higher AES. Furthermore, in contrast to the previous findings (Simón et al., 1995
), in the present study the highest embryo implantation rate was found in the group with highest peak E2 (group III).
In spite of the greater number of oocytes and embryos in groups II and III, the clinical outcomes were not significantly improved compared with group I, that is, rates of clinical pregnancy, spontaneous abortion and ongoing/delivered pregnancy per embryo transfer. Groups II and III appeared to have an increased rate of multiple pregnancies. While these differences did not reach statistical significance, the transfer of fewer embryos should be considered in these patient groups. Power calculations revealed that, with respect to clinical pregnancy rate and ongoing/delivered rate per embryo transfer, this study had a ß value of 0.72, and 384 patients in each group would be required in order to avoid a type II error (ß = 0.2). Therefore, although an increase in implantation rates was observed, no conclusions could be drawn at this time regarding the effects of high peak E2 levels on pregnancy outcome.
In conclusion, we consider that high peak E2 levels are not detrimental to oocyte quality, fertilization and embryo cleavage. These observations also suggest that sustained supraphysiological levels of E2 resulting from ovarian stimulation produce no adverse effects on the quality of developing human oocytes and embryos during IVFembryo transfer cycles. On the contrary, elevated levels result in higher implantation rates and a greater number of oocytes and embryos for selection at the time of embryo transfer, as well as for cryopreservation. These findings imply that the possible decrease in embryo implantation rates seen with conventional IVF (Paulson et al., 1990) is largely due to an endometrial effect.
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Notes |
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References |
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Submitted on June 22, 2001; accepted on September 27, 2001.