Leptin concentrations in normal women following bilateral ovariectomy

I.E. Messinis1,5, S.D. Milingos1, E. Alexandris2, I. Kariotis2,3, G. Kollios4 and K. Seferiadis4

1 Department of Obstetrics and Gynaecology, University of Thessalia, 22 Papakiriazi Street, 41222 Larissa, State Departments of 2 Obstetrics and Gynaecology and 3 General Surgery, Larissa and 4 Department of Biological Chemistry, University of Ioannina, Ioannina, Greece


    Abstract
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 Abstract
 Introduction
 Materials and methods
 Results
 Discussion
 References
 
To study the relationships between gonadal steroids and leptin, 20 women with normal cycles were investigated during the postoperative period following a laparotomy. Fourteen women underwent bilateral ovariectomy plus total hysterectomy either in the mid- to late follicular phase (n = 7, group 1) or in the early to midluteal phase (n = 7, group 2). The remaining six of the 20 women underwent cholocystectomy in the early to midfollicular phase of the cycle and were used as controls (group 3). In all three groups, serum leptin values decreased rapidly up to postoperative day 4. Then, leptin values increased significantly only in group 3 (P < 0.05). Leptin values before and after the operation showed significant positive correlations with body mass index (BMI), oestradiol and progesterone. However, with multiple regression analysis, BMI was the only parameter significantly correlated with leptin in group 3 (days 0 and 4–7), whereas in groups 1 and 2 progesterone and BMI showed independent significant correlations with leptin (days 0 and 8, r = 0.601 and r = 0.602 respectively). These results demonstrate for the first time a significant reduction in leptin concentrations in normal women following bilateral ovariectomy. Although BMI seems to be the predominant factor, it is also suggested that oestradiol and progesterone may participate in the control of leptin production during the human menstrual cycle.

Key words: body mass index/leptin/oestradiol/ovariectomy/ovary


    Introduction
 Top
 Abstract
 Introduction
 Materials and methods
 Results
 Discussion
 References
 
Leptin, a 16 kDa protein, is a product of the ob gene secreted by adipose tissue (Zhang et al., 1994Go). In humans, the secretion of leptin is performed in a pulsatile fashion (Licinio et al., 1998Go). This protein appears to regulate fat stores and body weight by decreasing appetite and increasing thermogenesis (Halaas et al., 1995Go; Caro et al., 1996aGo). The ob/ob mice that lack leptin become very obese and infertile and develop insulin resistance. However, fertility is restored in these animals after treatment with recombinant leptin (Barash et al., 1996Go; Chehab et al., 1996Go). In humans, the relationship between leptin and obesity is rather obscure. An association between obesity and leptin resistance has been proposed (Caro et al., 1996bGo; Schwartz et al., 1996Go), but the mechanism is not clear. Recently, a leptin binding factor has been identified in human serum which may influence the physiological response to leptin (Diamond et al., 1997Go). On the other hand, although human obesity has not been linked to mutations of the leptin gene, recently a mutation of the gene for leptin was detected in two severely obese children with very low serum leptin concentrations (Montague et al., 1997Go).

Since obesity is one of the symptoms in a significant proportion of women with polycystic ovary syndrome (PCOS), several studies have investigated changes in leptin concentrations in this syndrome (Brzechffa et al., 1996Go; Chapman et al., 1997Go; Laughlin et al., 1997Go; Mantzoros et al., 1997Go; Rouru et al., 1997Go). Although the results are conflicting, it has become evident that leptin may play a role in certain cases of PCOS and may act as a link between fat and reproduction. Recent studies have detected leptin receptor mRNA in the human ovary and specific binding of leptin in ovine granulosa cells (Cioffi et al., 1996Go; Spicer and Francisco, 1997Go), whereas experiments in vitro have shown that leptin may directly affect follicle stimulating hormone (FSH)-induced production of oestradiol by rat granulosa cells (Zachow and Magoffin, 1997Go). A possible relationship between oestrogen and leptin has been recently postulated. In rats, ovariectomy reduced significantly serum leptin values and the expression of ob gene in certain sites of white adipose tissue, changes which were reversed by oestradiol supplement (Shimizu et al., 1997Go; Yoneda et al., 1998Go). In humans, lower concentrations of leptin have been found in postmenopausal compared to premenopausal women and in men compared to pre- or postmenopausal women, while during the normal menstrual cycle the concentrations of leptin are higher in the luteal than in the follicular phase (Hardie et al., 1997Go; Shimizu et al., 1997Go; Messinis et al., 1998Go).

The present study was undertaken to examine further the relationships between gonadal steroids and leptin in normal women by investigating changes in leptin concentrations following bilateral ovariectomy.


    Materials and methods
 Top
 Abstract
 Introduction
 Materials and methods
 Results
 Discussion
 References
 
Patients
The study included 20 normally cycling women who volunteered for the study and gave written informed consent. Approval for the study was obtained from the local ethical committee. In all women ovulation was confirmed by ultrasound and serum progesterone measurement before admission to the study. Clinical and endocrine characteristics of the women are shown in Table IGo. All women were studied during the postoperative period following a laparotomy performed under general anaesthesia. Fourteen of them underwent bilateral ovariectomy plus total hysterectomy because of benign conditions of the genitalia, such as fibroids and menorrhagia, while the ovaries were normal. In seven of the 14 women (group 1), the operation was performed during the mid- to late follicular phases of the cycle, i.e when the size of the dominant follicle was 15–16 mm in diameter as assessed by ultrasound, and in the remaining seven (group 2) in the early to miduteal phase, i.e. 5 days after the spontaneous luteinizing hormone (LH) surge. The latter was detected by daily urine evaluation using kits for LH measurements (Organon LH color; Organon Hellas, Greece) and was confirmed by two or three blood samples on the day on which the test was positive. The remaining six of the 20 women were used as controls (group 3). These women underwent cholocystectomy for benign conditions of the gallbladder, such as chololithiasis, and the operation was performed in early to midfollicular phases of the menstrual cycle (days 4–7). All operations were performed in the morning (0800 h) and lasted less than 90 min. During the operation there were no complications and the loss of blood did not exceed 200 ml. The postoperative period was uneventful in all cases and the women were discharged home on postoperative days 7 (group 3) or 8 (groups 1 and 2). During the operation the presence of a dominant follicle in group 1 or a corpus luteum in group 2 was confirmed. Blood samples were taken from all women starting on the day of the operation, i.e. before the administration of the anaesthetic drugs and continuing initially every 12 h and then every 24 h up to the day of discharge. In all blood samples FSH, LH, oestradiol, progesterone and leptin concentrations were measured.


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Table I. Clinical and hormonal parameters of the women at the start of the study before the operation (day 0)
 
Hormone assays
For FSH and LH measurements, immunometric assays based on enhanced luminescence were used (Amerlite FSH and Amerlite LH assay respectively; Amersham International plc, Amersham, UK). The results are expressed as IU/l of standards calibrated against the WHO 2nd IRP of human FSH (58/549) and the 1st IRP of human LH (68/40). Oestradiol was measured using a competitive immunoassay based on enhanced luminescence (Amerlite Estradiol-60 assay; Amersham). The results are expressed as pmol/l. For progesterone measurement a competitive immunoassay was used (Kodak Amerlite Progesterone assay; Amersham). The results are expressed as nmol/l. Leptin was measured in all serum samples in duplicate using a radioimmunoassay method and all samples were assayed in one batch. Kits were purchased from Linco Research (RIA, Linco Research, St Charles, MO, USA) and contained human leptin antibody prepared in rabbit and raised against highly purified human leptin and standards and tracer prepared with human leptin. The results are expressed as ng/ml. The lower limits of detection for FSH, LH, oestradiol and progesterone were 0.5 IU/l, 0.12 IU/I, 50 pmol/l and 0.35 nmol/l respectively, while interassay and intra-assay coefficients of variation were 7.5 and 6.0%, 9.0 and 6.8%, 9.1 and 8.0%, and 7.0 and 6.6% respectively. The lower limit of detection for leptin was 0.5 ng/ml, while inter- and intra-assay coefficients of variation were 6.2 and 7.1% respectively.

Statistical analysis
For the purpose of the statistical analysis, hormone results were transformed into logarithms in order to achieve an approximately normal distribution of the data. However, in the results the arithmetic means are presented. For comparisons within the same group, statistical analysis was performed using one-way analysis of variance (ANOVA) and paired t-test as appropriate, while for comparisons between groups two-way ANOVA was used (effects of time and of women). Correlations between various parameters were calculated by using simple and multiple linear regression.


    Results
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 Abstract
 Introduction
 Materials and methods
 Results
 Discussion
 References
 
Serum concentrations of FSH and LH (mean ± SEM) before the onset of the operation (day 0) were significantly lower in group 2 (3.5 ± 0.7 and 2.1 ± 0.5 lU/l respectively) than in group 1 (6.8 ± 1.4 and 5.9 ± 1.1 IU/I respectively, P < 0.05) and group 3 (6.2 ± 0.6 and 5.4 ± 0.6 IU/I respectively, P < 0.05, Table IGo). Both FSH and LH values increased gradually but significantly from the day of the operation up to postoperative day 8 both in group 1 (32.9 ± 2.0 and 12.8 ± 1.7 IU/l respectively, P < 0.01) and group 2 (15.4 ± 3.1 and 5.5 ± 1.0 IU/l respectively, P < 0.01). Details of gonadotrophin changes in 10 of the 14 patients of groups 1 and 2 are reported elsewhere (Alexandris et al., 1997Go). In group 3, both FSH and LH showed a temporal but significant increase 12 h from the operation (P < 0.05, Figure 1Go). Then, both gonadotrophins decreased on day 2, remaining stable from day 3 to day 6 and increasing on day 7, indicating the onset of an LH surge.



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Figure 1. Serum concentrations (mean ± SEM) of leptin, oestradiol, progesterone and gonadotrophins in normally cycling women before and after bilateral ovariectomy plus total abdominal hysterectomy performed (day 0) ({circ}) in the mid- to late follicular phases (seven women, group 1) or ({bullet}) in the early to midluteal phase of the cycle (seven women, group 2). Another six women underwent cholocystectomy ({blacktriangleup}) in the early to midfollicular phase of the cycle. *P < 0.05, **P < 0.01, ***P < 0.001 (differences from the other two groups). FSH = follicle stimulating hormone; LH = luteinizing hormone.

 
Serum leptin values (mean ± SEM) on day 0 were significantly higher in group 2 (44.7 ± 4.5 ng/ml) than in groups 1 (22.7 ± 3.4 ng/ml, P < 0.05) and 3 (20.2 ± 3.8 ng/ml, P < 0.05, Table IGo, Figure 1Go) with no significant difference between groups 1 and 3. During the first 24 h following the operation, leptin values increased significantly in all three groups, peaking on day 1 (P < 0.05) and remaining on that day significantly higher in group 2 than in groups 1 and 3 with no significant difference between groups 1 and 3 (Figure 1Go). Then, leptin values declined significantly from days 1–4, gradually in groups 1 and 3 and more rapidly in group 2 (P < 0.01) with no significant difference between the three groups. From day 4 to day 7 or 8 after the operation serum leptin concentrations did not change significantly in groups 1 and 2, but increased significantly in group 3 (P < 0.05); however, there were significant differences between the three groups at any point (Figure 1Go).

Serum oestradiol values (mean ± SEM) on day 0 did not differ significantly between groups 1 (286 ± 57 pmo/l), 2 (270 ± 71 pmol/l) and 3 (227 ± 38 pmol/l), although they were lower in group 3. Oestradiol values decreased significantly in groups 1 and 2 at 12 h from the operation (P < 0.05) and further up to day 8 with no significant difference between the two groups at any point (Figure 1Go). In group 3, however, serum oestradiol values showed a temporal but significant increase on day 1 (P < 0.05), decreasing slightly on day 2 and then increasing gradually up to day 7 (500 ± 78 pmol/l, P < 0.01, Figure 1Go). Oestradiol values were significantly higher in group 3 than in groups 1 and 2 during the whole postoperative period (Figure 1Go). Serum concentrations of progesterone (mean ± SEM) were on day 0 significantly higher in group 2 (16.9 ± 1.2 nmol/l) than in group 1 (4.5 ± 0.6 nmol/l, P < 0.01) and group 3 (1.2 ± 0.2 nmol/l, P < 0.01). After the operation, in groups 1 and 2 progesterone values decreased rapidly during the first 24 h, particularly in group 2 and gradually thereafter up to postoperative day 8 with no significant difference between the two groups at any point (Figure 1Go). In group 3, serum progesterone values showed a temporal but significant increase 12 h from the operation (P < 0.01) remaining low throughout the rest of the postoperative period (Figure 1Go). The described changes in FSH, LH, oestradiol and progesterone concentrations during the postoperative period in group 3 (Figure 1Go) resemble those seen during the mid- to late follicular phase of the normal menstrual cycle.

A slight but non-significant decrease in body weight (mean ± SEM) was noted on postoperative day 8 compared with day 0 in group 1 (0.53 ± 0.20 kg) and group 2 (0.71 ± 0.30 kg) with no significant difference between the two groups. In group 3, the decrease in body weight on day 7 (3.2 ± 0.2 kg) was significantly greater than in groups 1 and 2 (P < 0.01). BMI values were available in groups 1 and 2 on days 0 and 8 and in group 3 on days 0, 4, 5, 6 and 7. BMI (mean ± SEM) did not change significantly on postoperative day 8 compared with the value on day 0 both in group 1 (26.1 ± 1.1 and 26.3 ± 1.1 kg/m2 respectively) and group 2 (26.7 ± 0.7 and 26.9 ± 0.8 kg/m2 respectively), while it decreased significantly in group 3 from day 0 (28.2 ± 1.5 kg/m2) to day 7 (26.3 ± 1.5 kg/m2, P < 0.001). A significant decrease in BMI was also seen in group 3 from day 0 to day 4 (27.3 ± 1.5 kg/m2, P < 0.001) and from day 4 to day 7 (P < 0.001). Serum leptin concentrations before and after the operation correlated significantly with BMI in groups 1 and 2 combined (r = 0.632, P < 0.001, n = 28, Figure 2bGo). A significant positive correlation between leptin and BMI was also found in group 3 before and after the operation (r = 0.892, P < 0.001, n = 30) (Figure 2aGo). Serum leptin values correlated significantly with oestradiol values from postoperative days 4–7 in group 3 (r = 0.480, P < 0.05, n = 24, Figure 3aGo) and from day 0 to day 8 in group 1 (r = 0.467, P < 0.001, n = 91, Figure 3cGo). In group 2, the correlations between leptin and oestradiol values were not significant. Significant positive correlations were also found between serum leptin and progesterone concentrations from postoperative day 0 to day 8 in group 1 (r = 0.239, P < 0.05, n = 91, Figure 3dGo) and group 2 (r = 0.217, P < 0.05, n = 91, Figure 3dGo) and from day 0 to day 7 in group 3 (r = 0.323, P < 0.05, n = 54, Figure 3bGo). When multiple regression analysis was performed in group 3, the significant positive correlation between leptin and oestradiol shown in Figure 3aGo was eliminated and leptin correlated significantly only with BMI. When leptin values on days 0 and 8 of groups 1 and 2 were combined, by simple regression analysis they correlated significantly with BMI (r = 0.602, P < 0.01, n = 28), progesterone (r = 0.601, P < 0.01, n = 28) and oestradiol values (r = 0.386, P < 0.05, n = 28). When multiple regression analysis was applied, the significant correlation of leptin with oestradiol was eliminated, while the correlation of leptin with progesterone and BMI were preserved. Oestradiol values correlated significantly with those of progesterone on days 0 and 8 in groups 1 and 2 (r = 0.520, P < 0.01, n = 28) and in group 3 from days 4–7 (r = 0.405, P < 0.05, n = 24). No significant correlations were found between BMI and oestradiol or progesterone values in all three groups of women.



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Figure 2. Correlations between serum leptin concentrations and body mass index (BMI) (a) in six cholocystectomized women (group 3) before the onset of the operation (day 0) and on postoperative days 4, 5, 6 and 7 (r = 0.892, P < 0.001, n = 30) and (b) in 14 ovariectomized women (groups 1 and 2) on days 0 and 8 (r = 0.632, P < 0.001, n = 28).

 


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Figure 3. Correlations between leptin and oestradiol or progesterone values in normally cycling women after bilateral ovariectomy plus hysterectomy performed in mid- to late follicular phases (seven women, group 1) or in early to midluteal phases (seven women, group 2) and in six women after cholocystectomy (group 3). Operations were performed on day 0. (a) Group 3, postoperative days 4–7 (r = 0.480, P < 0.05, n = 24), (b) group 3, days 0–7 (r = 0.323, P < 0.05, n = 54), (c) group 1, days 0–8 (r = 0.467, P < 0.01, n = 91) and (d) ({circ}) group 1, days 0–8 (r = 0.239, P < 0.05, n = 91) and ({bullet}) group 2, days 0–8 (r = 0.217, P < 0.05, n = 91).

 

    Discussion
 Top
 Abstract
 Introduction
 Materials and methods
 Results
 Discussion
 References
 
The present study is the first in which changes in leptin concentrations were investigated in women following bilateral ovariectomy. A significant reduction in leptin values was seen in both phases of the cycle during the week following the operation which, however, was preceded by a rapid increase during the first 24 h after the operation. This temporal increase in leptin values is difficult to explain. It seems rather unlikely that this is related to the abrupt decrease in oestradiol and progesterone concentrations as a similar temporal increase in leptin values was also seen in women of group 3, in whom serum oestradiol values, instead of declining, increased significantly. So far, oestradiol has been found to exert a stimulatory effect on leptin production in rats in vitro (Murakami et al., 1995Go). An explanation for this early increase in leptin values might be that during the incision of the abdominal wall manipulation of the s.c. fat tissue took place and as a result leptin was released in high amounts into the circulation, but this needs to be investigated. Finally, one cannot exclude the possibility that the early increase in leptin following the operation was a response to the surgical stress, as happened with gonadotrophins and gonadal steroids in this and previous studies (Messinis et al., 1996Go; Alexandris et al., 1997Go).

After the temporal increase, leptin values declined rapidly in all three groups of women up to postoperative day 4 to concentrations that were significantly lower than before the operation. At the same time, changes in oestradiol values varied considerably among groups, indicating that leptin changes during the immediate period following ovariectomy are independent of oestradiol. These results contradict data in rats in which treatment with oestrogen reversed the significant reduction in serum leptin concentrations and in the expression of ob gene in white adipose tissue that was seen 2–8 weeks after ovariectomy (Shimizu et al., 1997Go; Yoneda et al., 1998Go). It is evident, therefore, that factors other than oestradiol controlled leptin secretion during the postoperative period in the present study. Such factors could be changes in food intake, reduction in fat stores and body weight and consequently in BMI and decrease in motor activity. These factors, however, are interrelated and, although fat stores were not measured in the present study, only a small reduction in body weight with no significant changes in BMI was seen in the groups of ovariectomized women 1 week after the operation. Since body weight measurements were not available in the ovariectomized women during the greater part of the postoperative period, one cannot exclude the possibility that a significant reduction in body weight occurred in these women during the first 4 days after the operation at a time when great restrictions in food intake were applied. Although dramatic changes in leptin values in response to changes in food intake are not expected in normal or obese subjects (Korbonits et al., 1997Go), a recent study has shown that even a 4% reduction in body weight over a period of 7 days resulted in a 61% decrease in leptin values in men and women (Dubuc et al., 1998Go). These data, together with the significant positive correlations between leptin values and BMI that were seen in our patients during the postoperative period, indicate that changes in leptin values following the operation were predominantly determined by changes in this parameter.

The possibility, however, that oestradiol itself can affect leptin production in women is not excluded. A significant positive correlation between leptin and oestradiol values was seen during the second half of the postoperative period in the cholocystectomized women and this is in accordance with data in mid- to late follicular phase of the normal menstrual cycle (Messinis et al., 1998Go). At the same time, leptin and oestradiol values increased significantly in this group of women, even though BMI continued to decline. Furthermore, high affinity binding of 17ß-oestradiol in the cytoplasmic fraction of various white adipose tissues has been demonstrated in rats (Wade and Gray, 1978Go).

The finding that in ovariectomized women a significant independent association was found between progesterone and leptin values suggests that this steroid may also participate in the production of leptin by adipocytes. Significant positive correlations of leptin with progesterone were also found in a previous study during the normal menstrual cycle (Hardie et al., 1997Go). It is possible, therefore, that oestradiol during the follicular phase of the cycle primes the adipocytes to the stimulating effect of progesterone. This could explain the significantly higher values of leptin in the early to midluteal compared with the mid- to late follicular phase of the cycle seen in the present and in previous studies (Hardie et al., 1997Go; Shimizu et al., 1997Go; Messinis et al., 1998Go). The rapid decline of luteal phase leptin values after ovariectomy to concentrations similar to those of the follicular phase supports this assumption. The possibility that the ovaries may contribute to the circulating leptin concentrations in women cannot be excluded despite the fact that in this study leptin values declined both in the ovariectomized and the non-ovariectomized women. Recent data have suggested that the pre-ovulatory follicle itself may be an important source of leptin (Cioffi et al., 1997Go). Oestradiol and progesterone, therefore, may act within the follicle to increase leptin production at that site. On the other hand, leptin produced inside the ovary might act as a paracrine factor to affect steroid synthesis in the follicle and corpus luteum, since both binding of leptin and a direct effect of this substance on steroidogenesis have been demonstrated in vitro (Spicer and Francisco, 1997Go; Zachow and Magoffin, 1997Go). Alternatively, however, changes in leptin concentrations on days 0–4 in the group of cholocystectomized women could simply reflect the stage of the cycle in these women, i.e. early to midfollicular phase, during which a decline in leptin values has been recently described, although the mechanism is not clear (Messinis et al., 1998Go).

From a physiological point of view, these results support the hypothesis that leptin may be the missing link between body fat and reproduction (Conway and Jacobs, 1997Go). Apart from the relationship with gondal steroids, this protein may also affect reproduction through other mechanisms, such as by controlling early development of embryos before implantation (Antczak and Van Blerkon, 1997).

In conclusion, the present study confirms previous data that leptin concentrations are higher in the luteal than the follicular phase of the cycle. The results demonstrate for the first time that leptin concentrations following a laparotomy decline rapidly from the first to the fourth postoperative day both in ovariectomized and non-ovariectomized women. Although BMI seems to be the predominant factor responsible for these changes, it is also possible that oestradiol and progesterone are involved in the mechanism which controls the production of leptin during the normal menstrual cycle.


    Acknowledgments
 
We wish to thank Professor O.Tsolas, Director of the Department of Biological Chemistry, University of Ioannina for providing the laboratory facilities for the hormone assays.


    Notes
 
5 To whom correspondence should be addressed Back


    References
 Top
 Abstract
 Introduction
 Materials and methods
 Results
 Discussion
 References
 
Alexandris, E., Milingos, S., Kollios, G. et al. (1997) Changes in gonadotrophin response to gonadotrophin releasing hormone in normal women following bilateral ovariectomy. Clin. Endocrinol., 47, 721–726.[ISI][Medline]

Antczak, M. and Van Blerkom, J. (1997) Oocyte influences on early development: the regulatory proteins leptin and STAT3 are polarized in mouse and human oocytes and differentially distributed within the cells of the preimplantations stage embryo. Mol. Hum. Reprod., 3, 1067–1086.[Abstract]

Barash, I.A., Cheung, C.C., Weigle, D.S. et al. (1996) Leptin is a metabolic signal to the reproductive system. Endocrinology, 137, 3144–3147.[Abstract]

Brzechffa, P.R., Jakimiuk, A.J., Agarwal, S.K. et al. (1996) Serum immunoreactive leptin concentrations in women with polycystic ovary syndrome. J. Clin. Endocrinol. Metab., 81, 4166–4169.[Abstract]

Caro, J.F., Sinha, M.K., Kolaczynski, J.W. et al. (1996a) Leptin: the tale of an obesity gene. Diabetes, 45, 1455–1462.[ISI][Medline]

Caro, J.F., Kolaczynski, J.W., Nyce, M.R. et al. (1996b) Decreased cerebrospinal-fluid/serum leptin ratio in obesity. A possible mechanism for leptin resistance. Lancet, 348, 159–161.[ISI][Medline]

Chapman, I.M., Wittert, G.A. and Norman, R.J. (1997) Circulating leptin concentrations in polycystic ovary syndrome. Relation to anthropometric and metabolic parameters. Clin. Endocrinol., 46, 175–181.[ISI][Medline]

Chehab, F.F., Lim, M.E. and Lu, R. (1996) Correction of the sterility defect in homozygous obese female mice by treatment with the human recombinant leptin. Nature Genet., 12, 318–320.[ISI][Medline]

Cioffi, J.A., Shafer, A.W., Zupancic, T.J. et al. (1996) Novel B219/0B receptor isoforms: possible role of leptin in hematopoiesis and reproduction. Nature Med., 2, 585–589.[ISI][Medline]

Cioffi, J.A., Van-Blerkom, J., Antczak, M. et al. (1997) The expression of leptin and its receptors in pre-ovulatory human follicles. Mol. Hum. Reprod., 3, 467–472.[Abstract]

Conway, G.S. and Jacobs, H.S. (1997) Leptin: a hormone of reproduction. Hum. Reprod., 12, 633–635.[Free Full Text]

Diamond, F.B. Jr., Eichler, D.C., Duckett, G. et al. (1997) Demonstration of a leptin binding factor in human serum. Biochem. Biophys. Res. Commun., 233, 818–822.[ISI][Medline]

Dubuc, G.R., Phinney, S.D., Stern, J.S. and Havel, P.J. (1998) Changes of serum leptin and endocrine and metabolic parameters after 7 days of energy restriction in men and women. Metabolism, 47, 429–434.[ISI][Medline]

Halaas, J.L., Gajawala, K.S., Maffei, M. et al. (1995) Weight-reducing effects of the plasma protein encoded by the obese gene. Science, 269, 543–546.[ISI][Medline]

Hardie, L., Trayhurn, P., Abramovich, D. and Fowler, P. (1997) Circulating leptin in women: a longitudinal study in the menstrual cycle and during pregnancy. Clin. Endocrinol., 47, 101–106.[ISI][Medline]

Korbonits, M., Trainer, P.J., Little, J.A. et al. (1997) Leptin levels do not change acutely with food administration in normal or obese subjects, but are negatively correlated with pituitary–adrenal activity. Clin. Endocrinol., 46, 751–757.[ISI][Medline]

Laughlin, G.A., Morales, A.J. and Yen, S.S.C. (1997) Serum leptin levels in women with polycystic ovary syndrome: The role of insulin resistance/hyperinsulinemia. J. Clin. Endocrinol. Metab., 82, 1692–1696.[Abstract/Free Full Text]

Licinio, J., Mantzoros, C., Negrao, A.B. et al. (1998) Human leptin levels are pulsatile and inversely related to pituitary–adrenal function. Nature Med., 3, 575–579.[ISI]

Mantzoros, C.S., Dunaif, A. and Flier, J.S. (1997) Leptin concentrations in the polycystic ovary syndrome. J. Clin. Endocrinol. Metab., 82, 1687–1691.[Abstract/Free Full Text]

Messinis, I.E., Milingos, S., Kollios, K. et al. (1996) Changes in pituitary response to gonadotropin-releasing hormone following bilateral ovariectomy in women treated with follicle-stimulating hormone. Gynecol. Endocrinol., 10, 383–390.[ISI][Medline]

Messinis, I.E., Milingos, S., Zikopoulos, K. et al. (1998) Leptin concentrations in the follicular phase of spontaneous cycles and cycles superovulated with follicle stimulating hormone. Hum. Reprod., 13, 1152–1156.[Abstract]

Montague, C.T., Farooki, I.S., Whitehead, J.P. et al. (1997) Congenital leptin deficiency is associated with severe early-onset obesity in humans. Nature, 387, 903–908.[ISI][Medline]

Murakami, T., Iida, M. and Shima, K. (1995) Dexamethasone regulates obese expression in isolated rat adipocytes. Biochem. Biophys. Res. Commun., 214, 1260–1267.[ISI][Medline]

Rouru, J., Anttila, L., Koskinen, P. et al. (1997) Serum leptin concentrations in women with polycystic ovary syndrome. J. Clin. Endocrinol. Metab., 82, 1697–1700.[Abstract/Free Full Text]

Schwartz, M.W., Peskind, E., Raskind, M. et al. (1996) Cerebrospinal fluid leptin levels: relationship to plasma levels and to adiposity in humans. Nature Med., 2, 589–593.[ISI][Medline]

Shimizu, H., Shimonura, Y., Nakanishi, Y. et al. (1997) Estrogen increases in vivo leptin production in rats and human subjects. J. Endocrinol., 154, 285–292.[Abstract]

Spicer, L.J. and Francisco, C.C. (1997) The adipose obese gene product, leptin: evidence of a direct inhibitory role in ovarian function. Endocrinology, 138, 3374–3379.[Abstract/Free Full Text]

Wade, G.N. and Gray, J.M. (1978) Cytoplasmic 17 ß-[3H]estradiol binding in rat adipose tissues. Endocrinology, 103, 1695–1701.[Abstract]

Yoneda, N., Saito, S., Kimura, M. et al. (1998) The influence of ovariectomy on ob gene expression in rats. Horm. Metab. Res., 30, 263–265.[ISI][Medline]

Zachow, R.J. and Magoffin, D.A. (1997) Direct intraovarian effects of leptin: impairment of the synergistic action of insulin-like growth factor-I on follicle-stimulating hormone-dependent estradiol-17ß production by rat ovarian granulosa cells. Endocrinology, 138, 847–850.[Abstract/Free Full Text]

Zhang, Y., Proenca, R., Maffei, M. et al. (1994) Positional cloning of the mouse obese-gene and its human homologue. Nature, 372, 425–432.[ISI][Medline]

Submitted on August 28, 1998; accepted on January 7, 1999.