1 Department of Obstetrics and Gynaecology, 2 Department of Pathology, Research Institute Growth and Development (GROW) and 3 Department of Pharmacology, Cardiovascular Research Institute Maastricht3 (CARIM), Maastricht University, Maastricht, The Netherlands
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Abstract |
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Key words: angiogenesis/chorioallantoic membrane/endometrium/vascular density index
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Introduction |
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The purpose of this study was to determine whether human endometrial fragments obtained at different stages of the menstrual cycle are able to induce angiogenesis. The chick embryo chorioallantoic membrane (CAM), which is an established model for studying angiogenesis (Auerbach et al.1991; Knighton et al.1991
; Cockerill et al.1995
), was used to assess the angiogenic potential of endometrium. This in-vivo angiogenesis model permits detailed quantitation of the vascular response in an objective and reproducible way (Maas et al.1999
), allowing comparison of the angiogenic activity during different stages of the menstrual cycle.
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Materials and methods |
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Endometrium was placed in sterile saline and stripped of blood clots using fine forceps. Subsequently the endometrial tissue was carefully sectioned into fragments of 1.5x2 mm with the aid of a dissecting microscope and explanted at random onto the CAM, within 2 h after obtaining the tissue. Each CAM contained only one fragment. Endometrial fragments were explanted on 311 CAMs, depending on the amount of available tissue, which ultimately resulted in a total of 158 CAMs.
Mouse skin tissue was used as a negative tissue control (Petruzelli et al.1993; Maas et al.1999
) and processed similarly prior to explantation (8 CAMs). In addition, fragments of 1.5% agarose gel (12 CAMs) similar in size to the endometrial explants were used as negative controls. Similar fragments of low melting point agarose gel containing 10 ng transforming growth factor ß1 (TGFß1), a well known angiogenic factor (3 CAMs), were used as positive controls (Yang and Moses, 1990
). Additional controls consisted of normal unmanipulated CAM (12 CAMs).
CAM model
The assay and method of quantitation used have been described previously (Maas et al.1999). In short, fertilized eggs were incubated for 3 days at 37°C, 55% relative air humidity. At day 3 of incubation a rectangular window was made in the eggshell. The window was covered with Scotch tape to prevent dehydration. The eggs were returned to the incubator until day 10 when test materials were placed on the CAM. During further incubation for 4 days the developing vasculature of the CAM was observed each day under a stereomicroscope. At day 14 colour slides of the treated area were taken through a microscope. Subsequently the CAM was excised and fixed with 3.7% buffered formalin. Paraffin sections were cut and stained with haematoxylin and eosin for histological evaluation.
Analysis
Each of 193 CAMs was photographed in ovo. The Quantimet 570 image processing and analysis system (Leica, Cambridge, UK) displayed the slide on a monitor. The vascular density index (VDI) was calculated as an estimate of the overall vascular response to the various explants. A grid containing concentric circles was superimposed on the computerized image. The innermost circle was 2 mm in diameter and the others were 0.25 mm apart. The number of intersections between the vessels and the first five circles was determined (47.124 mm total circumference). Quantitation was done at a standardized 42x final magnification. Using a computerized image analysis system it was possible to count each image of the in-vivo situation twice under identical circumstances. The average of the two counts was taken as the VDI for that particular CAM. The difference between the two counts was used to assess the intra-observer variability. We calculated a mean difference of the two counts of 0.07 with a standard deviation of 13.6. The mean VDI of all observations (n = 386) was 266.2. The intra-observer variation was 5.1%.
Statistics
The median VDI was determined for each of the 22 endometrial samples. In advance of any data analysis, these median VDIs were divided into 4 menstrual cycle stages. In the classic 28-day cycle the early proliferation lasts through the seventh day and the late proliferative stage through the fourteenth day (Noyes et al.1950). The secretory phase was divided accordingly into early and late secretory stage. Based on the number of days since the onset of the last menstrual period, the early proliferative stage contained endometrium of cycle day 2, 4, 5, 6 and 7 (6 patients); the late proliferative stage, cycle day 8, 9, 10, 11,12 and 14 endometrium (7 patients); the early secretory stage, endometrium of cycle day 15, 16, 18 and 21 (4 patients) and the late secretory stage contained cycle day 22, 23, 26 and 27 (5 patients). Data are presented as box-and-whisker plots showing 10th and 90th percentiles, the 25th and 75th percentiles, medians and outliers. The KruskalWallis analysis of variance was used to compare the different stages of the menstrual cycle. A second non-parametric test, the MannWhitney U-test, was used to compare the late proliferative stage with each of the other stages and to compare the controls with each other and with each of the menstrual stages. P
0.05 was considered to reflect statistical significance.
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Results |
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Morphologically, we observed a clear directional growth pattern of the newly formed vessels towards the endometrium explants. Neither penetration of the vessels into the endometrium nor invasion of the endometrium into the CAM was observed. The explants consisted of viable endometrial stromal as well as glandular cells. Most of the viable stromal cells could be found at the periphery of the explant tissue, along the epithelial lining of the CAM (Figure 1D). These observations were similar in all experimental groups.
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Discussion |
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The primary aim of our study was to determine whether endometrial fragments at different stages of the menstrual cycle were able to induce angiogenesis. To investigate cyclic variation, the endometrial samples were categorized into four stages of the menstrual cycle on the basis of the number of days since the onset of the last menstrual period. Data on the histological appearance of the endometrium were not available. These data might have made the cutoff between, particularly, early and late proliferative samples less arbitrary.
Given the physiological and pathological importance of angiogenesis, much effort has been devoted to the isolation, characterization, and purification of factors that can either stimulate or inhibit angiogenesis (Klagsbrun and D'Amore, 1991). As for endometrium, most attention has been paid to vascular endothelial growth factor (VEGF) (Charnock-Jones et al.1993
; Li et al.1994
; Shifren et al. 1996
; Torry et al.1996
; Donnez et al. 1998
; Gargett et al. 1999
). Since endometrial growth and function is controlled by sex steroids, the influence of these steroids on VEGF expression has been studied (Charnock-Jones et al.1993
; Shifren et al.1996
; Greb et al.1997
; Huang et al.1998
). From these studies, it has not become clear whether VEGF regulates endometrial angiogenesis throughout the menstrual cycle, nor how steroids affect this process.
According to the hypothesis that changes in the balance of inducers and inhibitors of angiogenesis may activate the angiogenic switch (Hanahan and Folkman, 1996), it is highly unlikely that only one factor is responsible for the angiogenic potential of a specific tissue. Therefore, we reasoned that, in addition to studying individual angiogenic factors, the overall angiogenic activity of endometrium should be studied in a bioassay. Rogers and co-workers used an in-vitro angiogenesis bioassay to demonstrate the production of an endothelial migratory signal by cultured human endometrial explants (Rogers et al.1992
). The authors showed that two significant peaks of migratory signal occur, one during early proliferative phase and one during mid-late proliferative phase. There was a significant drop in early-mid proliferative phase. However, no increase of endometrial endothelial cell proliferation across the menstrual cycle could be identified in a subsequent immunohistochemical study (Goodger and Rogers, 1994
). Therefore, Rogers and co-workers suggested that endometrial angiogenesis occurs by elongation and intussusception rather than sprout formation (Rogers et al.1998
). In the present study we quantified the angiogenic response by using a standardized stereological technique to obtain the VDI (Harris-Hooker et al.1983
; Dusseau and Hutchins, 1988
; Maas et al, 1999
). The VDI obtained in this way has been considered a function of both vessel number and length (Dusseau and Hutchins, 1988
). However, Strick and co-workers, comparing different morphometric measurements of vascularity, concluded that VDI is a function of vessel length rather than number of vessels (Strick et al.1991
). By assessing the VDI in an in-vivo bioassay as a measure of endometrial angiogenesis we took into account the possibility that elongation of existing vessels, which is the non-sprouting type of angiogenesis (Risau, 1997
), may be of importance in this process. We found a cyclic variation in angiogenic potential of human endometrium, with a nadir in the late proliferative phase and significantly higher levels in the other phases. These findings correspond with other reports in which increased expression of VEGF mRNA was found in the secretory endometrium as compared to proliferative endometrium. Maximum levels of VEGF mRNA expression in the glands and VEGF immunostaining in the stroma were found in the menstrual phase (Charnock-Jones et al.1993
; Shifren et al.1996
; Torry et al.1996
; Gargett et al.1999
). During the (pre)menstrual phase of the cycle both production and release of endothelins by endometrial cells are increased (Ohbuchi et al.1995
). These potent vasoconstrictors act on the spiral arterioles in the endometrium, resulting in reduction of oxygen tension in this tissue (Smith, 1998
). The endometrial stromal cells respond to this hypoxic state by producing increasing levels of VEGF (Popovici et al.1999
). The increase in VEGF levels in the early proliferative endometrium may be responsible for elongation of the existing vessels, essential for tissue reconstruction in this phase of the cycle. In the secretory phase, the high angiogenic potential of endometrium may be necessary for both elongation and coiling of the spiral arterioles. The role of VEGF in these processes is still not clear. During the late proliferative phase angiogenesis is mainly required for further outgrowth and maintenance of the vasculature. Evidently, for this event, the angiogenic potential does not need to be as high as for elongation and coiling of the vessels in the other phases of the menstrual cycle.
In the present study we have focused on normal endometrial angiogenesis. Moreover, this model offers possibilities for studying angiogenesis dependent disorders, such as endometriosis. The appearance of the blood vessels radiating towards the endometrial fragment on the CAM, as demonstrated in this study, resembles the typical endometriotic lesions surrounded by a hypervascularized area seen at laparoscopy. It has to be elucidated whether endometrial fragments, that reach the peritoneal cavity during menstruation, are angiogenic and able to induce their own blood supply for further development into an endometriotic lesion.
In conclusion, human endometrium is angiogenic throughout the menstrual cycle. This increased angiogenic potential shows a cyclic variation, which is in accordance with the physiological changes of endometrium as well as endometrial vasculature during the cycle.
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Acknowledgements |
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Notes |
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References |
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Submitted on September 4, 2000; accepted on April 19, 2001.