3 Glycobiology Research and Training Center, Departments of Medicine and Cellular & Molecular Medicine, University of California, San Diego, La Jolla, CA 92093-0687
Received on November 15, 2003; revised on November 19, 2003; accepted on November 24, 2003
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Abstract |
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Key words: comparative biology / domain-specific adaptation / human evolution / sialic acid / Siglec
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Introduction |
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Mammalian cellexpressed Sias can be recognized by organism-extrinsic receptors, like viral hemagglutinins and bacterial toxins, as well as by organism-intrinsic receptors, such as the Siglecs (Sia-recognizing Ig-like lectins) and selectins (Angata and Brinkman-Van der Linden, 2002; Angata and Varki, 2002
; Crocker, 2002
; Crocker and Varki, 2001
; Karlsson, 1998
; Rosen and Bertozzi, 1994
). There are some known examples wherein the single oxygen atom difference between Neu5Ac and Neu5Gc can markedly affect such recognition processes (Angata and Brinkman-Van der Linden, 2002
; Angata and Varki, 2002
; Blixt et al., 2003
; Collins et al., 1997
; Karlsson, 1998
; Kelm et al., 1994
). Thus human Sia-recognizing receptors, such as Siglecs, became candidates for evolutionary change following the human-specific loss of Neu5Gc and consequent increase in Neu5Ac expression.
Siglecs are cell surface type 1 transmembrane Itype lectins that are broadly classified into two groupsthe CD33/Siglec-3-related group (CD33rSiglecs, i.e., Siglecs-3, and -5 through -11 in humans) that are clustered in one genomic region (19q13.34), and another group, Siglecs-1, -2, and -4, that are more distantly related (Angata and Brinkman-Van der Linden, 2002; Crocker and Varki, 2001
; Vyas et al., 2002
). The extracellular portion of the CD33rSiglecs consists of two to five immunoglobulin (Ig)-like domains, the most N-terminal one being a Sia binding V-set (antibody variable region-like) Ig-like domain (Angata and Brinkman-Van der Linden, 2002
; Crocker and Varki, 2001
). Conserved features of this domain are required for Sia binding (Alphey et al., 2003
), and recognition specificity can be altered by relatively few amino acid changes (Yamaji et al., 2002
). These CD33rSiglecs are of interest with regard to evolutionary changes, because comparison of the syntenic genomic regions in humans and rodents show several differences (Angata et al., 2001a
). Furthermore, the human-specific loss of a critical arginine residue in a Siglec-like molecule (Siglec-L1; also within the CD33rSiglec gene cluster) abolished Sia binding after the last common ancestor with apes (Angata et al., 2001b
).
The selective expression of most CD33rSiglecs on granulocytes, monocytes, macrophages, and NK cells (Siglecs-3, -5, and -711) (Angata and Brinkman-Van der Linden, 2002; Crocker and Varki, 2001
) implicates them in regulating innate immune functions mediated by these blood cell types. Involvement in cellular signaling is indicated by induced phosphorylation of conserved tyrosine residues on their cytosolic tails, which in turn causes association with the phosphatases SHP-1 and/or SHP-2 (Crocker and Varki, 2001
; Taylor et al., 1999
; Ulyanova et al., 2001
).
The amino-terminal V-set Sia-binding sites of CD33rSiglecs on circulating innate immune cells are constitutively "masked" by interactions with Sias present on the same cell surface (Collins et al., 2002; Razi and Varki, 1998
, 1999
). "Unmasking" Siglecs expressed on human peripheral blood occurs on cellular activation (Razi and Varki, 1999
), and masking status is tied functionally to signaling events (Grobe and Powell, 2002
). It is unknown whether Siglecs simply oscillate between masked and unmasked states or bind to other Sias (not expressed on the same cell surface) after unmasking.
Despite all this information, the primary biological functions of CD33rSiglecs remain unknown. One possibility is that they are self-recognition molecules that prevent inappropriate activation of innate immune cells. An alternative hypothesis is that when unmasked they serve as detectors of invasive Sia-expressing bacteria (Crocker and Varki, 2001; Jones et al., 2003
). Several strains of pathogenic bacteria are known to express Neu5Ac on their surfaces (Angata and Varki, 2002
; Troy, 1992
; Vimr and Lichtensteiger, 2002
), thereby mimicking host cell surfaces and evading detection by both innate and adaptive immune systems (Jarvis, 1995
; Vimr and Lichtensteiger, 2002
; Wessels et al., 1989
). Unmasked Siglecs could thus provide a mechanism for the innate immune system cells to recognize these camouflaged bacteria. Sia-expressing pathogenic bacteria isolated from multiple mammalian species express Neu5Ac but never Neu5Gc (Angata and Varki, 2002
; Troy, 1992
; Vimr and Lichtensteiger, 2002
). Thus the pathogen-recognition hypothesis predicts that CD33rSiglecs on cells of the innate immune system must recognize Neu5Ac.
Recent studies (Blixt et al., 2003; Sonnenburg et al., unpublished data) indicate that human CD33rSiglecs are relatively indiscriminate in binding both Neu5Ac and Neu5Gc. Although this competence of human CD33rSiglecs for Neu5Ac binding is consistent with their postulated role as detectors of sialylated bacteria, we provide evidence suggesting that Neu5Ac recognition is actually a derived, human-specific condition. This condition apparently resulted from recent adaptive evolution away from the ancestral strong Neu5Gc preference still observed for great apes and is a logical consequence of human loss of Neu5Gc expression.
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Results and discussion |
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To address the possibility that human Siglecs have evolved different Sia-binding specificity from those of great apes, we compared the Neu5Ac- and Neu5Gc-binding ability of human, chimpanzee, and gorilla Siglec-9, one member of the CD33rSiglecs that is expressed on monocytes and granulocytes. To investigate possible differences in binding specificity of Siglec-9, we cloned the N-terminal Sia-binding regions of chimpanzee and gorilla Siglec-9 and fused them to the Fc-region of human IgG, resulting in recombinant soluble chimeric proteins (Siglec-9-Fc). The Neu5Ac- versus Neu5Gc-binding properties of these molecules were compared to those of human Siglec-9-Fc (Figure 1). These binding assays clearly demonstrate that although human Siglec-9 binds both Neu5Ac and Neu5Gc, the chimp and gorilla orthologs strongly prefer Neu5Gc. These data are consistent with the hypothesis of functional adaptation in members of the human CD33rSiglecs to accommodate Neu5Ac-binding following human Neu5Gc-loss.
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Siglecs on circulating blood cells from human and apes show marked differences in binding Neu5Ac or Neu5Gc bearing probes
To test if endogenously expressed CD33rSiglecs from the human, chimp, and gorilla display similar binding differences observed for recombinant Siglec-9, we examined Sia binding of experimentally unmasked endogenous Siglecs expressed on native blood cells from these three species. Binding of soluble multivalent probes bearing Neu5Ac or Neu5Gc in either the alpha2-3 or alpha2-6 linkage (Angata et al., 2001b) was quantified by flow cytometry. Remarkable differences between human and great ape Siglecs were observed. Unmasked Siglecs on human monocytes (data not shown) and granulocytes bound probes bearing either Neu5Ac or Neu5Gc (Figure 2, top). In striking contrast, the corresponding chimp cells showed strong binding to Neu5Gc (see arrows in Figure 2, bottom), with no detectable binding to Neu5Ac-bearing probes, in agreement with the binding properties observed for recombinant Siglec-9. This strict Neu5Gc preference was also observed in blood samples from three other chimps and one gorilla (data not shown). The Sia binding observed by flow cytometry of human blood leukocytes represents a composite of the properties of at least five different CD33rSiglecs expressed on these cell types (Siglecs-3, -5, -7, -9, and -10) (Crocker and Varki, 2001
), and we have recently found that great ape blood leukocytes also express these Siglecs (Hurtado-Ziola and Varki, unpublished data). Together these data suggest the ancestral state for the predominantly expressed ape neutrophil and monocyte-associated CD33rSiglecs was a strong preference for Neu5Gc, with human CD33rSiglecs subsequently undergoing functional adaptation to accommodate Neu5Ac binding in the absence of Neu5Gc.
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Domain-specific differences between the sequences of the N-terminal regions of human and ape Siglec-9
The genomic structure of the N-terminal region of Siglec-9 within all three species is identical, with the first exon encoding the signal peptide and the Sia-binding V-set domain and the second exon encoding the adjacent Ig-like C2-set domain (antibody constant domain-like). Comparison of the N-terminal sequences (Figure 3) shows multiple amino acid differences specifically in the 124 amino acid V-set domain (human-chimp identity = 94.4%; human-gorilla identity = 92.7%). In contrast, the 93 amino acids comprising the adjacent C2-set domains have the expected level of amino acid identity (99% for human-chimp and human-gorilla) (Figure 3). This disparity in identity of the V-set domain and adjacent C2-set domain in interspecies comparisons is consistent with human Neu5Gc loss leading to selection for changes specific to the V-set domain that altered Sia binding specificity.
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Evidence for adaptive evolution involving the V-set domain of Siglec-9
Pairwise comparisons of Siglec-9 V-set DNA sequences amongst the three genera show that the great majority (almost 80%) of total nucleotide differences are nonsynonymous (result in amino acid changes). Indeed, seven of the eight chimp-human base pair differences in the 372 base pairs encoding the V-set domain are nonsynonymous (Table I, see also Appendix). This bias in the Siglec-9 V-set domains suggests that these amino acid changes have been selected for. To quantitate this bias, the nonsynonymous substitution rate (dN) and the synonymous substitution rate (dS) were calculated. A dN/dS >1 indicates positive directional selection, and values closer to zero suggest purifying selection (Hughes and Nei, 1988; Messier and Stewart, 1997
). Table I shows the dN/dS values for human-chimp, human-gorilla, and chimp-gorilla comparisons of the Siglec-9 V-set or C2 -set domain. Although these values indicate that amino acid changes in the V-set domain are under selection in all three lineages, the dN/dS ratio of 2.28 between humans and chimps is clearly highest. In contrast to the evidence for positive selection in the V-set domains, low dN/dS ratios (0.12 between chimp and human) for the adjacent C2-set domains implicate purifying selection as the major evolutionary force acting on this domain. Although the small sizes of the regions involved preclude definitive statistical analyses of these differences, the high identity of a noncoding sequence immediately adjacent to the V-set encoding exon provides further evidence suggesting positive selection of amino acidchanging mutations specific to the Sia-binding V-set domain. In the case of humans, one of these selective forces was likely the loss of Neu5Gc and the subsequent need to allow Neu5Ac binding to restore the masked state.
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Evidence for episodic selection within the V-set of Siglec-9 in hominoids
When the Gorilla sequence is used as an outgroup, it is apparent that human Siglec-9 acquired approximately twice as many lineage-specific amino acid substitutions as chimpanzees since divergence from the Homo-Pan common ancestor, consistent with adaptive changes in human sialic acid biology (Figure 4). To explore this issue further, we sequenced the N-terminal region of Siglec-9 from a bonobo. The ancestors of bonobos (Pan paniscus) and chimpanzees (Pan troglodytes) were geographically separated 3.5 mya, due to the appearance of the Zaire (Congo) river (Myers Thompson, 2003
), and molecular data suggest the genetic divergence of these Pan species to be
2.5 mya (Horai et al., 1992
). Remarkably, despite the prolonged period of evolutionary independence (which represents almost half of the evolutionary time since the common ancestor of Homo and Pan), we found only one difference between the chimp and bonobo V-set sequences (i.e., only 13% of the total divergence between chimps and humans). This conservation between the Pan V-set domains contrasts with the divergences among the Pan, human, and gorilla clades (Figure 4, left tree). On the other hand, the C2-set domain tree shows minimal divergences (Figure 4, right tree). Comparison of these domain-specific trees illustrates the disparate selective pressures on these adjacent segments of Siglec-9. The V-set domain tree also indicates that rapid evolution is not a ubiquitous feature of this domain in African hominoids, as the bonobo and chimp are tightly clustered.
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Conclusions and perspectives
For human Siglec-9, we have directly shown functional adaptation towards Neu5Ac-binding, evidence of Sia-binding domain-specific divergence from the African great apes and a bias of nonsynonymous substitutions within this domain consistent with positive selection for mutations affecting receptor functionality. Experimental probing of multiple CD33rSiglecs present on human, chimp, and gorilla blood cells (Figure 2) indicates that many of these human receptors may have undergone a similar human-specific functional change, away from a strong preference for Neu5Gc. Indeed, in vitro binding studies do show significant binding of other recombinant human CD33rSiglecs (-3, -5, -7, and -10) to both Neu5Ac and Neu5Gc (Blixt et al., 2003; Sonnenburg et al., unpublished data). Additionally, initial analyses of recombinant human and chimp Siglec-7 indicate multiple amino acid changes in the V-set domain, a strong chimp Siglec-7 preference for Neu5Gc, and a human Siglec-7 accommodation of Neu5Ac (Sonnenburg et al., unpublished data). Overall, our data also indicate that endogenous Sias (rather than surface Neu5Ac molecules of bacterial pathogens) are the functional ligands of CD33rSiglecs. This in turn suggests that the endogenous Sia landscape is the major factor directing evolution of CD33rSiglec binding specificity. Meanwhile, it is possible that some of Neu5Ac-expressing pathogens are actually exploiting the recently evolved Neu5Ac binding ability of human Siglecs.
We have also mapped the amino acid differences between human and chimp for Siglec-7 and -9 onto the available Siglec-7 V-set structure (Alphey et al., 2003) and found no obvious changes that would rationally explain the observed difference in binding specificity (Sonnenburg et al., unpublished data). Although one or two amino acid changes could potentially change Siglec specificity, it is also possible that the observed differences are the cumulative result of several mutations that altered the Sia-binding pocket. This is supported by the observation that only 1 of the 13 sites of amino acid differences in human and chimp Siglec-7 and Siglec-9 V-set domains is in common, and this site does not appear to directly interact with the sialic acid (Sonnenburg et al., unpublished data). Overall, the human change can be best characterized not as a specific switch from Neu5Gc to Neu5Ac preference but as a relaxation of specificity to accommodate Neu5Ac binding, without loss of Neu5Gc binding. Thus human Siglec-7 and -9 have accommodated Neu5Ac binding by distinct molecular strategies, suggesting that many of the V-set amino-acid differences that distinguish the human and chimp orthologs are required for the differences in observed binding properties. Together, these facts make it rather difficult to predict which amino acid changes are responsible and design rational mutagenesis studies.
Few human proteins are known to have undergone rapid selection during anthropoid primate evolution (Enard et al., 2002; Goldberg et al., 2003
; Johnson et al., 2001
; Messier and Stewart, 1997
; Nadezhdin et al., 2001
; Wyckoff et al., 2000
). To our knowledge, none of these reports directly demonstrated biochemically human-specific functional adaptation, as we have done here. It remains to be seen if changes in CD33r-Siglec-binding specificity are the residual signature of a past selective sweep involving a pathogen that affected Sia biology, whether they are relevant to present human resistance or susceptibility to disease or if they had any secondary consequences for the evolution of other human-specific traits.
We also note that the evidence for rapid evolution in Siglec-9 is much less apparent when the human-chimp comparisons include both the highly conserved exon 2 and the divergent exon 1. The Siglec-9 dN/dS decreases from 2.28 to 0.67 when the C2-set domain is included with the V-set domain in the analysis. Indeed, analysis of the entire Siglec coding regions from human and chimp (an additional three exons) would likely yield a value that does not noticeably deviate from that expected under purifying selection. For example, the average dN/dS ratio for 19 other immune-related genes of humans and chimpanzees is 0.45 (Chen et al., 2001). Thus the evidence suggesting domain-specific accelerated evolution would have been overlooked in conventional comparisons using entire open reading frames or full-length cDNAs. With chimp genome sequencing now well under way (Fujiyama et al., 2002
; Olson and Varki, 2003
), large-scale efforts to identify additional chimp-human genetic differences are anticipated. Because most genes will likely show only a few amino acid differences between the two species, our data caution that functionally significant genetic differences will be missed unless an exon-by-exon comparative approach is taken, with an emphasis on the functionality of the protein domain(s) encoded by each exon.
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Materials and methods |
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Genomic PCR and cloning of chimp, gorilla, and bonobo Siglecs
The following primers were used for amplification of Siglec-9 from chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla), or bonobo (Pan paniscus) genomic DNA: chimp Siglec-9 and bonobo Siglec-9 (5'-GTTCTGAGAGAAGAACC-3' and 5'-GCCTTCTCCTTGGAAGACAG-3') and gorilla Siglec-9 (5'-CTCGGATCCCTGGCACCTCTAACCC-3' and 5'-ATCTCCTTGGAAGACAGTCATGG-3'). Polymerase chain reaction (PCR) was conducted using Expand-High Fidelity polymerase (Roche, Mannheim, Germany). PCR fragments were cloned into pCR2.1-TOPO (Invitrogen) and sequenced or sequenced directly from the genomic PCR products on an ABI Prism 310 Genetic Analyzer. Sequences were verified on multiple independent PCR products.
Sequence analysis
Alignments were prepared using CLUSTAL W. DnaSP (version 3.51) was used to calculate dN and dS for given pairs of sequences (Rozas and Rozas, 1999).
Construction of recombinant Siglec-Fc expression vectors
Vectors encoding recombinant fusion proteins consisting of the chimp or gorilla Siglec-9 N-terminus and the Fc region of human IgG (Siglec-Fc) were prepared as follows. The great ape Siglecs were subcloned by PCR using the following primers: 5'-CCCTCTAGAGCCACCATGCTGCTGC TGCTGCTGCCCCTGC-3', 5'-ATCTCCTTGGAAGACAGTCATGG-3'. PCR fragments were isolated, digested with XbaI, and cloned into Ek-Fc/pcDNA3.1() (Angata et al., 2001a), digested with XbaI and EcoRV. Siglec-Fcs were produced as previously described (Angata et al., 2001a
).
Sialylation of polyacrylamide-biotin probes
Fifty micrograms of a biotinylated-PAA probe (Glycotech, Rockville, MD) bearing multiple copies of Galß1-4GlcNAc was sialylated in a mixture of 10 µl 0.5 M 4-morpholine propane sulfonic acid, pH 7.4, 0.5 µl 10% sodium azide, 1 µl 1 U/µl calf-intestine alkaline phosphatase, 25 µl 10 mM CMP-Neu5Ac or CMP-Neu5Gc, and 50 µl water, to which was added 120 mU of the sialyltransferases ST6Gal-I (Calbiochem, San Diego, CA) or ST3Gal-III (gift from Eric Sjoberg; formerly of Cytel). Reactions were allowed to proceed at 37°C overnight. Probes were recovered by ultrafiltration using a Microcon-10 (Millipore, Bedford, MA) and resuspension in water. Sialylation was quantitated by acid release in 2 M acetic acid, 80°C, for 3 h; derivitization by 1,2-diamino-4,5-methylenedioxybenzene; and high-pressure liquid chromatography analysis as previously described (Sonnenburg et al., 2002).
Flow cytometry of peripheral blood leukocytes
Samples (1020 ml) of blood were collected in Vacutainers (Becton Dickinson, Piscataway, NJ) containing ethylenediamine tetra-acetic acid. Phosphate buffered saline (PBS)-washed cells were treated twice for 5 min in 5 volumes ACK lysing buffer (150 mM NH4Cl, 10 mM KHCO3, 0.1 mM ethylenediamine tetra-acetic acid, pH 7.2) to lyse red blood cells. Leukocytes were pelleted, washed, and used at 0.51 million/sample for flow cytometry. Samples were either incubated in 100 µl freshly prepared 2 mM sodium periodate in PBS for 30 min, on ice, in the dark, or were sham-treated in PBS. Periodate specifically truncates the side chain of Sias resulting in CD33rSiglecs unmasking on the cell surface (Razi and Varki, 1999), allowing them to be probed for the binding of Neu5Ac- or Neu5Gc-bearing probes (Razi and Varki, 1999
). Forward- and side-scatter dot plots were used to identify granulocyte, monocyte, and peripheral lymphocyte populations.
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Acknowledgements |
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Footnotes |
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2 Current address: Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO
Sequences reported here are deposited in GenBank, under accession numbers AY532661, AY532662 and AY532663.
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Abbreviations |
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References |
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