2 Department of Biochemistry, La Trobe University, Melbourne, Victoria 3086, Australia; 3 CSIRO Division of Health Sciences and Nutrition, 343 Royal Parade, Parkville, Victoria 3052, Australia; and 4 Department of Microbiology, La Trobe University, Melbourne, Victoria 3086, Australia
Received on April 13, 2003; revised on June 5, 2003; accepted on June 10, 2003
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Abstract |
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Key words:
Agrobacterium ATCC31749
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curdlan(13)-ß-glucan synthase
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membrane topology
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modeling
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Introduction |
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The various crd genes were originally detected by analysis of random TnphoA insertion mutants of the Agrobacterium strain that failed to stain blue on agar containing the (13)-ß-D-glucan-specific dye aniline blue (Stasinopoulos et al., 1999
). The essential nature of the crdS gene is thus based on the abolition of curdlan production following insertional inactivation of this gene. This assumption awaits verification because the organization of crdASC in an operon raises the possibility that the curdlan-deficiency of crdS:: TnphoA mutants is due to polarity of the mutations on the downstream gene, crdC, rather than to inactivation of crdS.
Here we report experiments that confirm that crdS is essential for curdlan production and that the curdlan synthase (CrdS) protein is located in the inner membrane of the bacterial envelope and may be part of a multimeric complex. The topology of CrdS is also investigated, revealing the presence of seven transmembrane helices and a large cytoplasmic domain bearing the substrate-binding and catalytic motifs. Components of the conserved D,D,D35QxxRW motif (Saxena and Brown, 1997) that have been implicated in the catalytic activity of family GT-2 glycosyl transferases are evaluated for their position in a 3D model of the catalytic domain of CrdS based on the crystallographic structure of SpsA, a soluble glycosyl transferase belonging to the GT-2 family.
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Results |
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CrdS is an inner-membrane protein that occurs in protein complexes
To further investigate the subcellular location of the CrdS protein, polyclonal antibodies were raised to a glutathione-S-transferase (GST)-fusion protein that contains an internal portion of CrdS (amino acid residues 84362) at its C-terminus (see Materials and methods). This portion of CrdS (designated CrdS) was chosen because it is relatively hydrophilic (Figure 1) and encompasses a domain of CrdS that shares greatest homology (up to 46% similarity) with the sequences of glycan synthases in family GT-2 with a repetitive action pattern. The GST-
CrdS antibodies were used to determine the subcellular location of CrdS in the native Agrobacterium strain. Periplasmic, cytoplasmic, and membrane fractions were prepared from disrupted cells of LTU50 and examined by SDSPAGE and western analysis (Figure 3A and B). The identity of each fraction was verified using antibodies to representative periplasmic (maltose-binding protein), cytoplasmic (secretory protein A), and membrane (signal peptidase) proteins (Figure 3C). When GST-
CrdS antibodies were used, an immunoreactive protein band of Mr
60 kDa was found only in the membrane fraction (Figure 3B). We conclude that this protein, of the same size and cellular location as that expressed in crdS+ E. coli (Figure 2B), is CrdS.
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The total membrane fractions prepared from Agrobacterium LTU50 were further analysed using blue native (BN)PAGE (Schägger et al., 1994), a procedure that has been used to identify native membrane protein complexes in mammalian cells (e.g., Dekker et al., 1998
) and more recently in Gram-negative bacteria (Bailey et al., 1999
; Aufurth et al., 2000
). Membrane proteins from LTU50 solubilized in 1% (w/v) digitonin and subjected to BNPAGE formed a number of prominent bands of Mr from
550 kDa to
80 kDa (Figure 5A). Western analysis using GST-
CrdS antibodies detected immunoreactive protein bands at Mr
420 kDa and Mr
500 kDa, compared with a Mr
60 kDa for the CrdS polypeptide (Figure 3B) indicative of protein complexes.
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When E. coli carrying pVS1575(crdS+) were induced with isopropyl-D-thiogalactopyranoside (IPTG) and converted to spheroplasts, they produced the full-length CrdS protein (Mr 60 kDa) detectable by western analysis (Figure 7, lane 1). This protein was susceptible to proteinase K digestion following ultrasonic lysis of the spheroplasts (lane 4). In contrast, intact spheroplasts that were treated with proteinase K produced a truncated CrdS protein of Mr
29 kDa (lanes 2 and 3). The mass of this protein is in accord with that predicted for the peptide encompassing TMIIIIV (residues 77399; 36.4 kDa) when the typically higher mobility of wild-type and truncated CrdS proteins (Figure 2A) is taken into account. It was concluded that the failure of proteinase K to digest the large hydrophilic region of CrdS is due to its intracellular location, confirming a significant feature of the predicted topology of the CrdS protein that is common to both models A and B (Figure 6).
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Over the C-terminus of CrdS, four of the eight fusion pairs located between residues T450 and K630 yielded strong reciprocal responses. These included two fusions (A578 and K630) close to the C-terminus that exhibited high LacZ activity, suggesting that this region of CrdS is cytoplasmic. This finding is in keeping with model B (Figure 6). Model B is also consistent with the high LacZ activities of the remaining two strong reciprocal fusions at K466 and V477. In the alternative model A, fusions at all four of these sites would be expected to display high PhoA activity, not high LacZ activity. In contrast to the strong reciprocity of the fusions at A578 and K630, those at position S558 in the C-terminal region exhibited high LacZ activity and also anomalously high PhoA activity. Boyd and Beckwith (1989) have shown that loss of positive charged residues can lead to loss of topological information. Positively charged residues are especially concentrated in the region after position S558 and may have led to the retention of the CrdS moiety with the fused PhoA in the cytoplasm.
Of the remaining reciprocal fusions, those at D506 and R514 were unusual in that they exhibited intermediate PhoA activities (60 U) rather than the high levels predicted by model B, and significant LacZ activities (
97 U). Similar phenomena have been attributed (Calamia and Manoil, 1992
; van Geest and Lolkema, 2000
) to a charged residue, arginine, in the case of CrdS, in the hydrophobic stretch that precedes these fusion sites, destabilizing the formation of the TM helix TMVII. This would lead, on one hand, to an inefficient export of the PhoA moiety into the periplasm and, on the other hand, to significant retention of the LacZ moiety in the cytoplasm. Finally, the fusion pair at T450 is of interest as it occurs in the middle of a hydrophobic region that, according to model B, does not constitute a bona fide TM helix (Figure 6). It has been shown (Bibi and Béjà, 1994
; Heldermon et al., 2001
) that fusions positioned after a start-transfer signal for a segment that inserts in the membrane without traversing it can produce fusion proteins that orient the PhoA moiety extracellularly. This would account for the anomalously high PhoA activity associated with the T450 fusion, a feature that is in marked contrast with the low PhoA activities exhibited by the more distal K466 and V477 fusions, both of which are located in a strongly hydrophilic region of the same segment. Overall these studies suggest that CrdS has an NoutCin topology with seven TM helices, as suggested by model B.
Model of the donor-binding region of CrdS
The three-dimensional structure of only one nucleotide monosaccharide-dependent glycosyltransferase currently classified in the GT-2 family has been solved. This is SpsA, a glycosyltransferase from Bacillus subtilis (Charnock and Davies, 1999). Although no substrate or product has yet been specified for SpsA, its crystal structure has been solved both with and without bound UDP-Mn2+ and UDP-Mg2+. Thus the active site structure of SpsA may serve as a prototype for the organization of the UDP-binding site in other family GT-2 inverting synthases, including CrdS. The programs Threader v.3 (Jones et al., 1992
) and Modeler 6v2 (Sali and Blundell, 1993
) were used to build a 3D model of a 241-amino-acid portion of the CrdS sequence. This portion of CrdS (i.e., amino acids 115356), located between TMIII and -IV, contains the conserved UDP-binding motif and the conserved D,D,D35QxxRW motif of the GT-2 glycosyl transferases with repetitive action (Saxena et al., 1995
; Keenleyside and Whitfield, 1996
; Saxena and Brown, 1997
). Figure 9A shows this model in a ribbon form with some of the key active-site amino acid residues depicted in stick form. The predicted secondary structure (using PSIPRED, available online at http://bioinf.cs.ucl.ac.uk/psipred) for CrdS and the experimental secondary structure of SpsA are more similar in the N-terminal region (i.e., the donor region) than in the C-terminal region (i.e., the acceptor region), and hence the topology of the N-terminal region of CrdS could reliably be expected to be as depicted in the model. This was confirmed by ProsaII (Sippl, 1993
), a program that estimates the compatibility of the sequence with a given fold. The unreliability of the C-terminal topology was also indicated by ProsaII. This was not unexpected because the C-terminal region confers acceptor specificity and also carries the QxxRW motif believed to be involved in action of repetitive GT-2 glycosyl transferases and is absent from SpsA.
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A noteworthy component in many glycosyltransferase families, including GT-2, is a DxD sequence that incorporates the D2 of the D,D,D35QxxRW motif and has been implicated in metal binding (Wiggins and Munro, 1998). In SpsA, Asp99 sits adjacent to the distal phosphate of UDP in the binding site and coordinates with the leaving group divalent metal (Mn2+), providing support for the conjectured role of this aspartate in metal binding (Charnock and Davies, 1999
). In the CrdS model, the equivalent amino acid is Asp210. Lys183 from the conserved HAKAGNLNN motif in CrdS (see Discussion) is positioned to interact with UDP in the model. Charnock and Davies (1999)
speculate that Asp191 of SpsA may be the catalytic base in the proposed inverting enzyme mechanism. The equivalent in CrdS is the conserved Asp304. The model does not shed light on the possible function of the conserved QxxRW motif, because this is located in the (unreliable) C-terminal region. The positions of the amino acids identified as contacts with the UDP portion of the substrate are indicated by arrows on the sequence of CrdS in Figure 9B.
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Discussion |
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Hydropathy analysis predicts that both prokaryotic and eukaryotic polysaccharide synthases of family GT-2 are membrane proteins (see Barny et al., 1996; Kawagoe and Delmer, 1998
). We have established immunochemically that CrdS is an inner membrane protein of Agrobacterium and of E. coli when expressed in this host (Figures 2 and 4). Membrane locations have also been established experimentally for synthases for hyaluronan from Group A streptococci (Stoolmiller and Dorfman, 1969
), including S. pyogenes (Heldermon et al., 2001
), and for S. pneumoniae SIII polysaccharide (Smith et al., 1960
; Cartee et al., 2000
) and inner membrane locations demonstrated for NodC chito-oligosaccharide synthase from Rhizobium leguminosarum (Barny and Downie, 1993
) and for the E. coli glucosyltransferase (MdoH) for periplasmic glucans (Debarbieux et al., 1997
).
The membrane topology of a number of family GT-2 bacterial polysaccharide synthases have been predicted (see Barny et al., 1996; Kawagoe and Delmer, 1998
), but experimentally determined membrane topologies are limited to the R. leguminosarum NodC (Barny et al., 1996
), E. coli MdoH (Debarbieux et al., 1997
), and HasA hyaluronan synthase from S. pyogenes (Heldermon et al., 2001
). Some information on the topology of the large hydrophilic region of Acetobacter xylinus cellulose synthase, AcsA, has also been obtained experimentally (Saxena et al., 1995
). From our fusion analyses we concluded that CrdS has seven membrane-spanning helices rather than the eight predicted by model A, and an NoutCin topology (Figure 6). The large hydrophilic region is oriented to the cytoplasm, a conclusion reinforced by experiments showing that a polypeptide encompassing this region remained intact when E. coli spheroplasts were treated with proteinase K (Figure 7). The NoutCin topology led to the prediction that a portion of CrdS in the C-terminal region occurred as a nonmembrane-spanning amphipathic helix (Figure 8). Such a feature also occurs in the HasA protein from S. pyogenes (Heldermon et al., 2001
), but its functional significance is not understood. The two other GT-2 enzymes whose membrane topologies have been investigated, NodC (Barny et al., 1996
) and HasA (Heldermon et al., 2001
), also have a large cytoplasmic region. In CrdS, NodC, and HasA, this region carries the D,D,D35QxxRW motif characteristic of family GT-2 transferases with repetitive action patterns (Saxena et al., 1995
; Saxena and Brown, 1997
; Keenleyside and Whitfield, 1996
). All residues of this motif are required for the catalytic activity based on site-directed mutagenesis of Saccharomyces cerevisiae chitin synthases (Nagahashi et al., 1995
; Cos et al., 1998
; Ruiz-Herrara et al., 2002
) and A. xylinus cellulose synthase (Saxena and Brown, 1997
; Saxena et al., 2001
).
Sequence alignment of the CrdS cytoplasmic region with bacterial cellulose synthase sequences shows that the three Asp residues in the D1,D2,D335QRxRW motif occur in DD1G/T, D2C/AD, and T/SED3 submotifs, respectively, and that there are additional common motifs, namely,Yx2Rx41;HAKAGNL/IN, QTPH/Q (Stasinopoulos et al., 1999), and PxVDx5YxE (Römling, 2002
) (Figure 9B). All of these motifs also occur in Bradyrhizobium japonicum (1
3)-ß-glucan synthase (NdvB; accession number AF047687) involved in cyclic (1
3,1
6)-ß-glucan production (Bhagwat and Keister, 1995
, Bhagwat et al., 1996
) and in a B. japonicum hypothetical protein (B112448) (Kaneko et al., 2003
). The hypothetical protein, in fact, has higher homology with CrdS than does NdvB (i.e., 40% sequence identity and 54% similarity over
500 residues versus 26% identity and 44% similarity over
300 residues, both including the cytoplasmic region). Two other motifs, FFCGS and Rx2FLx2PL, found in the bacterial cellulose synthases (Römling, 2002
) are poorly conserved in CrdS (Figure 9B), NdvB, and the hypothetical protein, giving support to the suggestion by Römling (2002)
that they may have a role in the determination of (1
4)-linkage specificity.
A model of the donor-binding domain of the CrdS protein was constructed by threading the sequence of the CrdS protein containing the UDP-binding site motif onto the SpsA structure. Amino acids in CrdS involved in binding UDP were predicted to correspond to those in SpsA. The topology of the UDP binding region found in the crystal structure of SpsA and deduced from the model of CrdS is also a feature of glycosyltransferases from families GT-7, -13, and -43, whose crystallographic structures have been determined (Tarbouriech et al., 2001).
The preliminary evidence from BNPAGE experiments suggests that CrdS is probably a component of a large complex that may be a homomultimer or a heteromultimer, perhaps involving CrdA and/or CrdC. These two proteins show no homology with transferases, hydrolases, nucleotide monosaccharide-metabolizing proteins, or transport proteins that have been found associated with other polysaccharide synthases, for example, for polysialic acid (Bliss et al., 1996) and the Group I polysaccharide from E. coli (Drummelsmith and Whitfield, 2000
), or for cellulose from A. tumefaciens (Matthysse et al., 1995
) and A. xylinus (Saxena et al., 1994
). The unique CrdA and CrdC if in a multimeric complex with CrdS perhaps assist transit of curdlan across the two membranes of the cell envelope. In rhizobia, the lipo-oligosaccharide Nod factors are synthesized at the cytoplasmic face of the inner membrane, and it is proposed that they are secreted by a functional complex encoded by the nodIJ genes (Vazquez et al., 1993
; Fernandez-Lopez et al., 1996
). In the case of the E. coli K5 capsular polysaccharide, four proteins are involved in its biosynthesis and there is evidence for the involvement of a stable heterooligomeric complex on the cytoplasmic membrane (Hodson et al., 2000
, Arrecubieta et al., 2001
).
In contrast to these examples, the polytopic HasA membrane protein of S. pyogenes (Heldermon et al., 2001) is able to synthesize and secrete to the cell surface the high-molecular-weight hyaluronan product without the involvement of other proteins. HasA function is, however, dependent on cardiolipin that in association with the synthase protein, perhaps creates a porelike passage for chain translocation (Tlapak-Simmons et al., 1998
, 1999
). In Agrobacterium, maximal curdlan production requires phosphatidylethanolamine (Karnezis et al., 2002
), which may be needed for correct folding or for maintenance of folding of one or more of the essential Crd proteins or perhaps may also be involved in creation of a porelike structure.
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Materials and methods |
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Growth media
Strains of E. coli were grown at 37°C and Agrobacterium at 28°C. Nutrient broth (NB), nutrient agar (NA), Agrobacterium defined broth (ADB), and aniline blue agar (ABA) was as described by Stasinopoulos et al. (1999) and Luria agar (LA) was as described by Sambrook et al. (1989)
. Supplements (mg/L) in NA were ampicillin (Ap, 100) and tetracycline (Tc, 15); those in ABA were chloramphenicol (Cm, 50) and tetracycline (10); and those in LA were 40 XP and 40 X-Gal.
Conjugal mobilization of plasmids and complementation studies
The mobilization of plasmids from E. coli S17-1 to Agrobacterium mutants was performed according to Stasinopoulos et al. (1999). For complementation analysis, the Agrobacterium transconjugants were selected on ABA containing Tc and Cm and were assessed after growth for 57 days. Blue-staining colonies indicated complementation (i.e., restoration of curdlan production), whereas unstained (white) colonies indicated the absence of complementation.
Expression of the Agrobacterium crdS gene in E. coli
The crdS and crdS genes of pVS1575 and pVS1574, respectively, were expressed (Tabor and Richardson, 1985
) from the T7
10 promoter following induction of the chromosomal T7 RNA polymerase gene of the E. coli host, BL21(DE3)(pLysS). The procedure was similar to that described (Ausubel et al., 1994
) except that the bacteria were induced with 1 mM IPTG for 30 min prior to the addition of rifampicin (200 µg/ml for 30 min at 37°C) and subsequent exposure to [35S]methionine-cysteine mix (10 µCi/ml for 5 min). Whole cell lysates or cell fractions were analyzed by SDSPAGE.
Preparation of cell fractions from Agrobacterium and E. coli
Agrobacterium cells harvested from a 5-day 200-ml ADB culture were washed three times in potassium phosphate buffer (50 mM, pH 7.4), resuspended in 30 ml 20% (w/v) sucrose buffer (50 mM TrisHCl, pH 8.0, 2 mM ethylenediamine tetra-acetic acid [EDTA]) and treated on ice for 30 min with lysozyme (0.2 mg/ml) prior to centrifugation (12,000 x g for 10 min). The supernatant containing periplasmic proteins was retained. The cell pellet was resuspended in 40 ml 50 mM TrisHCl (pH 8.0) and subjected to five cycles of freezing in liquid nitrogen and thawing on ice to lyse the cells. KCl was added (0.2 M final concentration) and unbroken cells removed by centrifugation (12,000 x g for 10 min). The clarified lysate was recentrifuged (150,000 x g for 1 h) to obtain the soluble fraction containing cytoplasmic proteins. The pellet, suspended in 400 µl TrisHCl (pH 7.5), served as the membrane fraction. Proteins in the periplasmic and cytoplasmic fractions were precipitated at 10% trichloroacetic acid (TCA) prior to analysis using SDSPAGE. A similar, scaled-down procedure was used for E. coli harvested from 10 ml of a log-phase (OD600nm of 0.60.8) NB culture that had been induced for 3 h with 0.5 mM IPTG.
Separation of inner and outer membranes of Agrobacterium and E. coli
The procedure was based on that described by Grahn et al. (2000). Agrobacterium cells and IPTG-induced E. coli cells were cultured and washed as described and resuspended in 15 ml buffer (10 mM TrisHCl [pH 7.4], 1 mM MgCl2, 10 µg/ml DNase). The cells were broken by three passages through a precooled French pressure cell at 47 mPa (N/m2) followed by incubation (4°C for 30 min) with lysozyme (0.1 mg/ml). After the intact cells were removed the total membrane fraction was collected from the supernatant by centrifugation (110,000 x g for 2 h), resuspended in 1 ml 10 mM TrisHCl (pH 7.4), layered on a continuous 2560% (w/w) sucrose gradient prepared in 10 mM TrisHCl, 1 mM EDTA (pH 7.4), and centrifuged in a Beckman SW40 rotor (40,000 x g for 16 h at 4°C). Fractions (0.5 ml) were collected from the bottom of the tube, and their refractive index and A280nm were measured. The content of the outer-membrane marker KDO (Keleti and Lederer, 1974
) and the activity of the inner-membrane enzyme NOX (Osborn et al., 1972
) was also determined. Proteins in each fraction were precipitated at 10% TCA prior to analysis by SDSPAGE.
Protein purification and preparation of CrdS polyclonal antibodies
An 833-bp region of crdS that encodes the cytoplasmic domain of CrdS from amino acid residues 97374 (Figure 1) was amplified by PCR (using primers 5'-CCTGAATTCGGATCCAGGCTTTATGCCATGGGGTCC and 5'-CGCGAATTCGTCGACTCCACGCCAGAGAGGTCC) and cloned into BamHI-SalI-digested pGEX-2T, creating a GST (gst)-crdS gene fusion (pVS1576). E. coli XL1-Blue (pVS1576) was grown in 1 L LB to an OD600nm of 0.60.8 and induced with IPTG (1 mM for 2 h). The harvested cells (
8 g wet weight) were resuspended in 10 ml 25% (w/v) sucrose lysis buffer (50 mM TrisHCl [pH 8.0], 1 mM EDTA, 2 mg/ml lysozyme). After 30 min incubation at 4°C, 1 µg/ml DNase was added together with 10 mM MgCl2 and 1 mM MnCl2; incubation continued for a further 30 min. Lysis of the cells was completed by the addition of 16 ml detergent buffer (1% [w/v] deoxycholic acid, 1% [v/v] Nonidet P-40, 20 mM TrisHCl [pH 7.5], 2 mM EDTA). The lysate was centrifuged at 12,000 x g for 10 min at 4°C, and the pellet, which contained most of the GST-
CrdS fusion protein, was collected, resuspended in 5 ml Triton X-100, and recentrifuged. This process was repeated until a compact pellet was obtained. The final pellet was resuspended in 20 ml 8 M urea; after 30 min at room temperature, the sample was centrifuged at 20,000 x g for 1 h at 4°C and the clarified protein solution filtered (pore size 0.22 µ) and stored at -70°C. Antibodies were prepared by subcutaneous injection of a rabbit with 150 µg of the GST-
CrdS protein administered first with Freund's complete adjuvant and at weeks 6 and 10 with Freund's incomplete adjuvant. Blood was collected at week 12 and the recovered serum stored at -20°C.
Electrophoresis, western blotting, and immunostaining
Extracts from Agrobacterium and E. coli were resolved by SDSPAGE (Ausubel et al., 1994) or BN-PAGE (Schägger et al., 1994
) and the proteins were transferred onto nitrocellulose or polyvinylinidinedifluoride filters using a semidry western transfer cell (BioRad, Hercules, CA). Immunoreactions were performed using the appropriate specific rabbit anti-serum anti-GST-
CrdS, anti-LacZ (gift of T. Lithgow) and anti-PhoA (from Eppendorf-5Prime, Inc., Boulder, CO) and horseradish peroxidasecoupled anti-rabbit antibodies (Sigma, St. Louis, MO), and were detected by the enhanced chemiluminescence method according to the manufacturer's instructions (Roche Diagnostics, Australia). Sizes of proteins were estimated by comparison with SeaBlue + pre-stained molecular mass markers (Novagen, Madison, WI). Antibodies to maltose-binding protein, SecA secretory protein, and signal peptidase were a gift of T. Lithgow.
Enzyme assays
Alkaline phosphatase and ß-galactosidase were measured according to Brickman and Beckwith (1975) and Miller (1972)
, respectively, using midlog phase (OD600nm of 0.60.8) cultures of plasmid-carrying E. coli CC118 that were grown in LB supplemented with 4% (w/v) glucose prior to induction with 1 mM IPTG for 1 h.
Spheroplast preparation and proteolysis with proteinase K
Ten-milliliter NB cultures of E. coli (pVS1575) in exponential phase (OD600nm of 0.6) were induced with 1.5 mM IPTG for 2 h and harvested; the cells were resuspended in 10 ml ST buffer (20 mM TrisHCl, pH 8.5) containing 20% w/v sucrose. Spheroplasts were prepared by the addition of EDTA (2 mM final concentration) and lysozyme (10 mg/ml) and incubated at 4°C for 20 min. The spheroplasts (8090% of the treated bacteria) were used immediately. A lysate of the spheroplasts was prepared by the addition of MgSO4 (15 mM final concentration) and sonication of the sample. Aliquots (1 ml) of spheroplasts and the spheroplast lysate were treated with proteinase K (0.2 or 0.4 mg/ml; Sigma) at 4°C for 30 min, and proteolysis was stopped by the addition of 5 mM phenylmethylsulphonyl fluoride. Deoxycholate was added to give a final concentration of 0.01% (w/v) and the proteins were precipitated by adding one-fifth volume of ice-cold 72% (w/v) TCA. The precipitated proteins were washed twice with acetone (4°C), air-dried and prepared for SDSPAGE.
Construction of a 3D model of the catalytic domain of CrdS
The fold recognition program Threader v.3 (Jones et al., 1992) was used to thread a 241-amino-acid portion of the CrdS sequence onto the X-ray crystallographic structure of SpsA (Protein Data Bank ID: 1QGQ), using a value of 1000 for the maximum number of considered paths and default values for all other parameters. The threaded sequencestructure alignment, after some manual readjustment, was used as input to the program Modeler 6v2 to build a 3D model.
This Modeler program (Sali and Blundell, 1993) builds models by satisfaction of spatial restraints derived from the alignment of the target with the template. Twenty structural models of CrdS were built using the following parameter values or options: library_schedule of 1, max_var_iterations of 500, md_level of "refine_4," repeat_optimization of 2, and max_molpdf of 106.
The model with the lowest value of the Modeler objective function (i.e., -1.0 x ln, the molecular probability density function) was selected as the best model structure. The structural quality of this model was checked with the programs PROSAII v. 3.0 (Sippl, 1993) and Profiles-3D (Lüthy et al., 1992
). The 3D-1D self-compatibility score from Profiles-3D for this model was 80.3, compared with an expected score of 109.5 for a protein structure of this length and with incorrect structures having scores below 49.3. The electrostatic potential was calculated and mapped onto the molecular surface of the model structure with the program GRASP v. 1.3.6 (Nicholls et al., 1991
) using formal charges on Asp, Glu, Arg, and Lys side chains and partial charges on backbone atoms.
Nucleotide sequence accession number
The nucleotide sequence of crdS was submitted to GenBank under the accession number AF057142.
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Acknowledgements |
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Footnotes |
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Abbreviations |
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References |
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