2CRC Department of Drug Development and 3CRC and University of Manchester Department of Medical Oncology, PICR, Christie Hospital, Manchester M20 4BX, UK
Received on February 15, 2000; revised on May 30, 2000; accepted on June 1, 2000.
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Abstract |
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Key words: heparan sulfate/oligosaccharides/FGF-1/FGF-2/activity
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Introduction |
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The HS binding site on FGF-1 has been identified by x-ray crystallography, with the major residues involved being N18, K112, K113, K118, and R122 (Zhu et al., 1991; Blaber et al., 1996
; DiGabriele et al., 1998
). The primary FGFR binding domain of FGF-1 has been less well studied; however, two FGFR binding sites (high and low affinity) have previously been identified on FGF-2 (Springer et al., 1994
). Recent crystallographic studies have shown the FGF-2 primary high affinity FGFR binding site to be conserved in FGF-1 (Y15, R35, N92, Y94, L133, and L135) with the exception of a Leu substitution for Met at position 135 (Blaber et al., 1996
). The second FGF-2 low affinity site in FGF-1 was also located and found to be composed of a seven-residue loop from positions K101 to H106 and the adjacent W107 (Blaber et al., 1996
). This loop is longer than the corresponding region in FGF-2 (Springer et al., 1994
), and it is the variation in the low affinity site which is believed to determine FGFR subtype specificity (Seddon et al., 1995
).
HS chains are highly complex molecules with very heterogeneous structure. This heterogeneity is due to a series of structural modifications, which occur after the initial synthesis of the repeating -GlcUAß1,4GlcNAc- polymer precursor. The subsequent modification pathway proceeds in a stepwise manner, beginning with the N-deacetylation and N-sulfation of some regions of the chains (Lindahl, 1989). These sulfated domains (S-domains) are then further transformed by epimerization of GlcUAß1,4- to IdoUA
1,4-, followed by 2-O-sulfation of selected IdoUA residues. Further alterations include 6-O-sulfation of GlcNAc/GlcNSO3 (Gallagher et al., 1986
; Lindahl and Kjellen, 1987
) also the more infrequent 3-O-sulfation of GlcNSO3 (Marcum et al., 1986
; Pejler et al., 1987
; Edge and Spiro, 1990
) and 2-O-sulfation of GlcUA (Lindahl et al., 1994
). S-domains are separated by regions of low or zero sulfation, in which the main disaccharide repeat is unmodified GlcUAß1,4GlcNAc (Turnbull and Gallagher, 1991
; Lyon et al., 1994
; Pye and Kumar, 1995
). Several studies have shown that HS chain structure differs extensively depending on the cell type (Turnbull and Gallagher, 1990
; Kato et al., 1994
; Lyon et al., 1994
; Sanderson et al., 1994
; Pye and Kumar, 1995
), or the tissue it is extracted from (Maccarana et al., 1996
). Changes are also seen in malignant transformation (Winterbourne and Mora, 1981
; Pejler and David, 1987
; Jayson et al., 1998
), development (David et al., 1992
; Brickman et al., 1998a
), aging (Feyzi et al., 1998
), and injury (Challacombe and Elam, 1995
).
Various groups have identified sulfated oligosaccharide sequences (S-domains) within heparin and HS that interact strongly with FGF-1. These oligosaccharides were found to be enriched in IdoUA(2S)1,4GlcNSO3(6S) disaccharides, with their affinity for FGF-1 increasing with 2-O- and 6-O-sulfate content (Mach et al., 1993
; Ishihara, 1994
; Fromm et al., 1997
; DiGabriele et al., 1998
). Indeed, an x-ray crystallography study of FGF-1 and heparin showed a direct role for 2-O and 6-O-sulfates in the FGF-1 interaction (DiGabriele et al., 1998
). This is in contrast to HS interaction with FGF-2, in which there is no role for 6-O-sulfation in the interaction with the primary HS binding site (Faham et al., 1996
). The minimum length of oligosaccharide binding to FGF-1 with relatively high affinity and promoting its mitogenic activity has been reported as being an octa- or decasaccharide (Mach et al., 1993
; Ishihara, 1994
; Fromm et al., 1997
). Chemically desulfated heparins have also been utilized, in order to show the importance of 2-O- and 6-O-sulfate groups in the promotion of FGF-2 and FGF-1 mitogenic activity (Guimond et al., 1993
; Ishihara et al., 1995
, 1997). Intracellular signaling by FGFs is believed to be initiated by FGFR dimerization and a number of models have been proposed, by which HS/FGF/FGFR interact to form a signaling complex. These models include the oligomerization of FGF-1 by HS, thereby presenting FGF-1 dimers/oligomers to FGFRs (Mach et al., 1993
; Spivak-Kroizman et al., 1994
). Another model based on the interaction of HS with the extracellular region of FGFR-1 (Kan et al., 1993
) proposes that HS acts as a bridge between FGF-2 and FGFR in the ternary complex (Guimond et al., 1993
). In a more recent model, it was suggested that a monomeric complex of HS and FGF-2 facilitate receptor dimerization through two FGFR-binding interfaces (Pantoliano et al., 1994
; Springer et al., 1994
). We have also reported that monomeric complexes of HS and FGF-2 are the minimum functional unit for FGF-2 activity (Pye and Gallagher, 1999
).
Recent structure-activity studies using desulfated heparins have showed the importance of 2-O- and 6-O-sulfation of heparin in mediating the mitogenic activity of FGF-1 (Guimond et al., 1993; Ishihara et al., 1995
, 1997). However, no detailed structure/activity data is available for the growth factors more appropriate, cell surface HS receptor, which has considerably more variability in chain structure and sulfation pattern than heparin. In this study, we have generated a library of HS oligosaccharides separated on the basis of molecular size and content/pattern of sulfation. This library was then used to determine structural features of HS-oligosaccharides, which mediate the mitogenic activity of FGF-1.
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Results |
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Comparisons of the disaccharide analysis and the activity data for the dodecasaccharides did not display the clear relationship between 6-O-sulfate content and FGF-1 stimulatory activity (Figure 6C) as seen with the decasaccharide fractions (Figure 6B). For example, the most sulfated dodecasaccharide fractions tested dp12F and dp12G had lower FGF-1 activatory ability than dp12E, even though they had a considerably higher 6-O-sulfate content (Figure 6C). Moreover, the dp12D fraction, which has approximately half the 6-O-sulfate content of dp12F, had similar FGF-1 stimulatory activity. However, perhaps the most significant observation on structure/activity relationships was seen when comparing the composition of the most powerful FGF-1 activating dodecasaccharide fraction dp12E, and non-activating dp12B fraction. Only small variations in total 6-O, 2-O, and N-sulfate content were observed in these preparations despite the differences in biological activity. Analysis of the dp12E fraction by size exclusion chromatography showed that the deviation from the correlation between 6-O-sulfate content and biological activity, seen with the deca- and other dodecasaccharides, was not a result of cross-contamination with longer oligosaccharides (Figure 7). Relating the relevant levels of dodecasaccharide FGF-1 stimulatory activity to the content of individual disaccharide components proved difficult with no clear correlation being observed. Differences in composition and activity between FGF-1 and FGF-2 were also difficult to ascertain with no particular disaccharide contributing significantly to differential activity. Thus qualitative and quantitative characteristics of the dodecasaccharides determine their activation properties; this applies to both FGF-1 and FGF-2 as discussed below.
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Discussion |
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Heparinase III is believed to produce resistant HS oligosaccharides with the general structure HexUA
1,4-(GlcNSO3(±6S)
1,4IdoUA(±2S))N
1,4GlcNR with R being Ac or SO3 (Linhardt et al., 1990
). Recently, however, it has been suggested that the enzyme works more extensively to produce fragments enriched in IdoUA(2S)-containing disaccharides (Desai et al., 1993
; Yamada et al., 1995
). This information along with our disaccharide compositional analysis suggests that the oligosaccharide fractions used in this study contain a backbone of IdoUA(2S)
1,4GlcNSO3(±6S) disaccharides. A correlation was initially observed between total sulfate content (as judged by SAX elution times) and FGF-1 promoting activity with the dodeca-, deca, and octasaccharide fractions tested, and in particular with the decasaccharide fractions. Disaccharide analysis generally showed this to be a result of increasing 6-O-sulfate level, with the main contributor being the disaccharide IdoUA(2S)
1,4GlcNSO3(6S). Although some notable variations were seen with 2-O- and N-sulfate levels of the octasaccharide fractions they could not be clearly related to changes in FGF-1 stimulatory activity. The data in this study therefore implicate 6-O-sulfation in playing an important role in the modulation of FGF-1 activity. These results are supported by others who showed that chemically 6-O-desulfated heparins were unable to promote FGF-1 or FGF-2 activity (Guimond et al., 1993
; Ishihara, 1994
; Ishihara et al., 1995
). Little, however, is known about the sulfation patterns of naturally occurring cell surface HS and their abilities to affect FGF-1 activities. The exact role that 6-O-sulfate groups play in the final assembly of the FGF-1/HS/FGFR signaling complex is also unclear, although a role for 6-O-sulfate groups in the interaction with FGF-1 has been suggested (Ishihara, 1994
; DiGabriele et al., 1998
). In the case of FGF-2, it has been proposed that HS oligosaccharides acts as a bridge between FGF-2 and the FGFR (Guimond et al., 1993
; Rusnati et al., 1994
), with the 6-O-sulfate groups interacting with the HS binding site on the receptor (Kan et al., 1993
; Pantoliano et al., 1994
).
The presence of nonactivating/inhibitory oligosaccharides was an interesting finding. Additionally, since each oligosaccharide fraction is comprised of a mixture of structures, the different levels of FGF-1 promoting activities seen are likely to be due to the relative amounts of activating and inhibitory oligosaccharides present in each fraction. As suggested previously for FGF-2 (Pye et al., 1998) the direct correlation between the 6-O-sulfate content of an HS-decasaccharide fraction and its biological activity implies that the occurrence of 6-O-sulfate groups in specific positions is necessary for the promotion of FGF-1 mitogenic activity. The increased frequency of occurrence of 6-O-sulfate(s) at precise position(s) is presumably reflected in the increased activity of the more 6-O-sulfated decasaccharides. However, it could be argued that the relationship between 6-O-sulfate content of HS oligosaccharides and FGF-1 biological activity is purely a result of a nonspecific requirement for a high negative charge density. Indeed, it has previously been shown using chemically desulfated heparins, that substantially higher levels of 6-O-sulfation were required for FGF-1 promoting activity than with FGF-2 (Ishihara et al., 1995
). In the present study, however, the case for specific sulfate positioning was strengthened by the data obtained from the analysis of the dodecasaccharide fractions, in which it was clearly seen that only relatively low levels of 6-O-sulfation were needed for FGF-1 stimulatory activity. Also, and perhaps even more supportive, is the fact that small differences in 6-O- sulfate content of the dodecasaccharide fractions resulted in considerable diversity in FGF-1 mitogenic promoting activities. Although no specific positional requirement for 2-O- sulfation could be seen with the dodeca- and decasaccharide fractions, the presence of obligatory 2-O-sulfate groups cannot be ruled out, on account of a significant divergence in the total 2-O-sulfate levels of the individual octasaccharide fractions.
This study has also yielded novel information on differential activation of FGFs by HS oligosaccharides. It was plainly seen with the octasaccharide fractions, that chain length could play a role in determining specificity in differential FGF-1/FGF-2 activation. A role for sulfation pattern changes in the contrasting ability of HS to affect FGF-1 and FGF-2 activities was also implicated, by the dodecasaccharide fractions capacities to differentially modulate either FGF-1 or FGF-2 action. Indeed, it has been shown that neural cells are able to switch from FGF-2 to FGF-1 mediated potentiating activity at a crucial stage in neuronal development (Nurcombe et al., 1993). This has recently been shown to be a result of controlled variation in HS chain structure (Brickman et al., 1998a
). Changes in HS sulfation patterns have been associated with a variety of biological processes and disease states, with the most notable changes occurring in the 6-O-sulfate content of HS (Winterbourne and Mora, 1981
; Pejler and David, 1987
; Pejler et al., 1987
; David et al., 1992
; Kato et al., 1994
; Brickman et al., 1998a
,b; Feyzi et al., 1998
; Ghiselli et al., 1998
; Jayson et al., 1998
; Safaiyan et al., 1998
; Salmivirta et al., 1998
), with the main contributor to these variations being GlcNSO3(6S) containing disaccharides. This indicates a possible regulatory role for the HS biosynthetic pathway, and in particular with the sulfotransferase enzyme controlling 6-O-sulfation within heparinase III resistant domains. One possible explanation therefore for the differential FGF activating characteristics of the HS oligosaccharides used in this study, may again lie in the precise positioning of specific O-sulfate groups. For example, 6-O-sulfate groups are required for the interaction of FGF-1 with HS, but not for the interaction with FGF-2 (Ishihara, 1994
; DiGabriele et al., 1998
). Other positional differences may also influence different FGF activatory capacities, for instance it is believed that 6-O-sulfate groups play a role in HS interactions with FGFRs (Kan et al., 1993
; Pantoliano et al., 1994
). However, it is not known if a specific 6-O-sulfate position is conserved for this purpose within HS oligosaccharides on the formation of different FGF ternary complexes.
Changes in the expression of different FGFR variants have been implicated in the ability of cells to modulate their activities to various members of the FGF family. Our results show that variation in the fine structure of domains within HS chains may also allow cells to control their positive and negative responses to individual FGFs and in addition to change their specificity towards promotion of different growth factors. This study also shows promise for the pharmaceutical development of bio-active oligosaccharide analogues that can discriminate between individual members of the FGF family.
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Materials and methods |
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Preparation of HS oligosaccharides
The HS oligosaccharide fractions utilized in this study were the same samples used previously (Pye et al., 1998) and were produced as follows. Porcine mucosal HS (200 mg) was exhaustively digested by heparinase III 50 mIU in 100 mM sodium acetate, 0.5 mM calcium acetate buffer pH 7.0 for 24 h at 37°C. The progress of the enzyme was monitored by absorbance at 232 nm, and further additions of enzyme were made until digestion was complete. The digestion products were then size separated using a Bio-Gel P-6 column (170 cm x 1.5 cm). Samples were eluted at a flow rate of 6 ml/h in 0.5 M ammonium bicarbonate and 1 ml fractions collected. Peaks were detected by measuring the absorbance of fractions at 232 nm, pooled separately, and freeze-dried. Size fractionated samples were then further resolved by strong anion-exchange (SAX) HPLC, as described previously for dodeca- and decasaccharides (Pye et al., 1998
). These gradient conditions were varied for the octasaccharide mixture, with saccharides being eluted using a 0.041.3 M linear NaCl gradient in Milli Q water pH 3.0 over 1 h at a flow rate of 1 ml/min. Fractions (1 ml) were collected and the elution profile monitored by absorption at 232 nm. Oligosaccharide-containing fractions were then pooled as indicated in Figure 1. Oligosaccharides were next freeze-dried and desalted by application to a Sephadex G-25 column eluted with water. Finally, samples were quantified by drying to constant weight and by measuring their absorbance at 232 nm.
F32 cell mitogenesis assay
BaF3 cells transfected with FGFR1 (designated F32 cells; Ornitz et al., 1992) were routinely maintained in RPMI-1640 medium, 10% horse serum supplemented with interleukin-3 conditioned medium (prepared from WEHI 3b cells) at 37°C, 5% CO2. For the assay system F32 cells were plated into 96-well plates at a density of 50,000 cells/well in 100µl RPMI-1640 medium supplemented with 10% horse serum and the appropriate growth factor + oligosaccharide test samples. Cells were incubated for 46 h before addition of [3H]thymidine (0.3 µCi/well) for a further 2 h. Incorporation of [3H]thymidine was stopped by harvesting cells on a Filtermate-196 cell harvester. Plates were allowed to air dry, before addition of 25µl of Microscint O to each well and incorporated radioactivity counted on a top count system (Packard, Pangbourne, Berks, UK).
Strong anion-exchange HPLC of disaccharides
The HS oligosaccharide fractions were depolymerized to disaccharides by complete digestion with a mixture of heparinases I, II, and III followed by Bio-Gel P-2 chromatography as described previously (Turnbull et al., 1992). The resulting disaccharides were then resolved by SAX-HPLC on a ProPac PA1 analytical column (25 x 0.48 cm, Dionex). The column was first equilibrated with Milli Q water (adjusted to pH 4.0 with HCl), samples were then injected and the disaccharides resolved using a two stage NaCl gradient (00.12 M over 90 min followed by 0.121.0 M over 45 min) in Milli Q water pH 4.0. The elution positions of specific disaccharides, detected by absorbance at 232 nm, were established by comparison with authentic standards.
Analytical size exclusion HPLC
The oligosaccharide fractions obtained by SAX-HPLC of the sized oligosaccharide mixtures were applied (2550 µg in 20µl of Milli Q water) to three TSK columns connected in series (TSK3000PW 30 x 0.75 cm, TSK3000SW 60 x 0.75 cm and TSK2000SW 60 x 0.75 cm). The columns were eluted with 0.5 M NaCl at a flow rate of 0.6 ml/min, and the elution profiles monitored by absorbance at 232 nm, 0.005 AUFS. Retention times were determined for each oligosaccharide fraction and the void and total volumes measured using dextran blue and potassium dichromate.
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Acknowledgments |
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Abbreviations |
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Footnotes |
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References |
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