From the Diabetes and Endocrinology Research Group (X.J.C., G.W.), University of Liverpool, Liverpool; and the Divisions of Neuroscience (M.L.E., R.A.L.) and Vascular Biology (C.A.L., J.R.S.A., S.W.), SmithKline Beecham Pharmaceuticals, Harlow, U.K.
Address correspondence and reprint requests to Dr. Xue J Cai, Diabetes and Endocrinology Research Unit, University of Liverpool, Liverpool L69 3GA, U.K. E-mail: xjcai{at}liv.ac.uk .
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ABSTRACT |
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INTRODUCTION |
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The neurochemical identities of the LHA neurons and pathways that mediate
hypoglycemia-induced hyperphagia remain uncertain. The LHA contains
glucose-sensitive neurons that are stimulated by hypoglycemia, and these
account for 25% of the LHA neurons
(4,5).
Hypoglycemia mainly activates LHA glucose-sensitive neurons indirectly
(6), and pathways ascending
from the brainstem are thought to be particularly important. These include a
projection from the nucleus of the solitary tract (NTS)
(7,8),
which relays information from vagal afferents including glucoreceptors in the
gut and liver (9). About 75% of
recorded NTS neurons respond with altered electrical activity to blood glucose
fluctuations within the physiological range
(10).
Recent evidence suggests that some of the LHA neurons that respond to hypoglycemia express the peptides known as orexins (11) or hypocretins (12). The hypocretins have sequences similar to but longer than orexins. Hypocretin-1 includes the orexin-A sequence with five extra amino acids at the NH2-terminus and one at the COOH-terminus, whereas hypocretin-2 has one extra residue at the COOH-terminus of the orexin-B sequence (12). Orexin-A and -B are 33- and 28-residue peptides, respectively, derived from prepro-orexin (13), and were named for their ability to stimulate feeding when injected intracerebroventricularly or into the LHA (13,14,15,16,17,18,19). Orexins are expressed solely in a neuronal population that is restricted to the perifornical LHA and zona incerta (20,21,22,23,24). This region has reciprocal connections with numerous hypothalamic and extrahypothalamic structures involved in feeding behavior, including the hypothalamic paraventricular nucleus (PVN) and arcuate nucleus (ARC) and the NTS (20,24).
We and others previously showed that hypothalamic pre-pro-orexin mRNA levels were increased by prolonged fasting and by insulin-induced hypoglycemia when food was not available (13,25). We suggested that orexin neurons are stimulated by falling blood glucose levels but inhibited by food ingestion and that they might participate in the on-off regulation of short-term feeding behavior (25). It is now clear that orexin neurons are those previously identified as containing "prolactin-like immunoreactivity," which are known to be activated by hypoglycemia (26).
Here, we explored further the relationship between hypoglycemia, food intake, and orexin neuronal activation and the possible mediatory role of the NTS. We again induced acute (5-h) hypoglycemia with insulin in groups of rats that were either allowed to eat freely or were fasted throughout. We first used the early response gene marker Fos to map neuronal activation in the LHA, PVN, ARC, and NTS together with immunohistochemical identification of orexin neurons. In a parallel experiment, we used specific radioimmunoassays to measure changes in whole hypothalamic orexin-A and -B concentrations under the same experimental conditions.
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RESEARCH DESIGN AND METHODS |
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Tissue and sample preparation. The rats were deeply anesthetized with a lethal dose of Hypnorm/Hypnovel (3 mg/kg), and blood was collected and centrifuged. Plasma was stored until assayed for plasma glucose using an autoanalyzer (glucose oxidase method; Yellow Springs Instruments, Yellow Springs, OH). The rats were perfused transcardially with 0.9% saline containing heparin (20 U/ml), followed by ice-cold fixative solution containing 4% paraformaldehyde in 0.01 mol/l phosphate-buffered saline (PBS). Brains were removed, post-fixed overnight by immersion in the same fixative, and cryoprotected with 0.5 mol/l sucrose for 24 h at 4°C. Brains were rapidly frozen in dry ice-cooled isopentane and sliced into 50-µm coronal sections on a cryostat (-23°C). Free-floating sections were rinsed several times and stored in immunobuffer (0.01 mol/l PBS containing 0.3% Triton X-100 and 0.12% thimerosal) at 4°C.
Immunohistochemistry. A preliminary study using chromogenic double-labeling detection of Fos and orexin immunoreactivities (i.e., avidin-biotin complex plus diaminobenzidine for Fos, and Vector blue alkaline phosphatase detection for orexin) did not in all cases allow us to determine whether Fos-positive (Fos+) nuclear profiles lay within orexin-containing cells, especially where cell bodies were strongly orexin-positive. To overcome this difficulty, we developed a fluorescent double-labeling protocol similar to that used by others to study colocalization of receptors and Fos+ neurons (27). This technique demonstrated patterns of Fos and orexin immunoreactivities that were identical to those we obtained using chromogenic detection and are similar to previous reports (28,29). The fluorescence method in combination with confocal analysis proved to be more powerful than histochemical detection methods because it revealed greater subcellular and three-dimensional (3-D) detail. In particular, we could interrogate the confocal 3-D volume in cases where the association of Fos and orexin was ambiguous (see "Image analysis.").
Fluorescence double-labeling was performed on every fifth frontal section through the hypothalamic region that contained orexin neurons and on every tenth frontal section through the medulla at the level of the NTS. Sections were rinsed three times in immunobuffer before incubation with avidin D solution followed by biotin solution (Vector Laboratories, Burlingame, CA) to block endogenous biotin-binding sites.
The sections were incubated overnight with a polyclonal sheep anti-Fos antibody (1:3,000; Genosys, Cambridge U.K.), then for 2 h with biotin-SP-conjugated donkey anti-sheep IgG (1:500; Jackson ImmunoResearch, West Grove, PA), followed by Alexa 488-conjugated NeutrAvidin (1:1,000; Molecular Probes, Eugene, OR) for 2 h. Sections were next incubated overnight in a rabbit polyclonal antibody against a partial sequence (amino acids 14-33) of orexin-A (1:1,000; raised in-house at SmithKline Beecham, as reported before [29]), followed by Cy5-conjugated donkey anti-rabbit IgG (1:400; Jackson ImmunoResearch) for 2 h. Before each incubation, the sections were washed thoroughly three times for 10 min in immunobuffer. All incubations were at room temperature. Finally, the sections were mounted on cover slips in Citifluor (Citifluor, London, U.K.), sealed, and stored at 4°C. The specificity of the anti-orexin serum was tested by preincubating the primary antiserum with excess synthetic orexin-A (50 µg/ml) for 2 h before use or by omitting the primary antiserum from the incubation steps. In both cases, selective staining of cell profiles was abolished (29). To test for specificity of the Fos antiserum, the primary antiserum was omitted from the incubation procedure, and this resulted in no selective immunostaining.
Fluorescence double-labeling was visualized using a laser scanning confocal microscope (Leica TCS-SP; Leica, Heidelberg, Germany). Alexa 488 was excited by the 488-nm laser line and emitted light collected at 540 ± 35 nm to reveal Fos immunoreactivity. Cy5 was excited using the 633-nm laser line and emitted light collected at 675 ± 35 nm to reveal orexin immunoreactivity. With this arrangement, no cross talk was observed between the detection channels, so scanning and detection of Alexa 488 and Cy5 were performed simultaneously.
Image analysis. For each region to be analyzed, a series of 8-12 optical sections at intervals of 1.0-1.2 µm was examined to generate a brightest-point projection single image containing both Fos and orexin immunoreactivity. For the LHA, Fos+ nuclei were counted and categorized as whether or not they lay within an orexin-containing neuronal cell body; in cases where coincidence was ambiguous, a 3-D volume image was constructed and rotated to clarify the relationship. Counts were expressed as the number of nuclei per 500 x 500-µm square field, bounded medially by the fornix and extending laterally toward the internal capsule and dorsally toward the zona incerta. For the PVN, dorsomedial hypothalamic nucleus (DMN), and ARC, sections were similarly imaged and Fos+ nuclei counted in identified anatomical areas contained within a 1,000 x 1,000-µm square field. Results were expressed as means ± SE nuclei/mm2 (bilaterally, 8-10 sections each animal).
Brainstem sections were examined in a dark field using a dissecting microscope (Leica FL3) to determine the rostrocaudal level relative to Bregma according to the atlas of Paxinos and Watson (30). Sections were first viewed using differential interference contrast (Leica DMR; Leica, Heidelberg, Germany) to identify neuroanatomical boundaries and then viewed in fluorescence mode to count Fos+ nuclei. Using DIC, the boundary between the NTS and the dorsal motor nucleus of the vagus (DMNX) was identified by the larger cell bodies of the DMNX. Separate counts were made for the DMNX and NTS and expressed as total numbers in these smaller areas.
Experiment 2.
Animals. Three groups of Sprague-Dawley rats (n =
8) were given the same treatment as in experiment 1 and were killed 6 h after
injection. The hypothalamus was immediately dissected en bloc under a
binocular microscope from a frontal slice of fresh unfixed brain tissue
between the optic chiasm and the mammillary bodies, cutting horizontally below
the anterior commissure and vertically through the perihypothalamic sulci
(31). The hypothalamus was
boiled in 0.1 mol/l hydrochloric acid for 10 min to extract peptides, and the
supernatant was kept at -80°C until assayed for protein concentrations
(BCA protein assay kit; Pierce, Rockford, IL) and orexins.
Radioimmunoassays. Hypothalamic orexin-A and -B levels were
measured using commercially available radioimmunoassay kits (Peninsula
Laboratories, San Carlos, CA). Neither the orexin-A nor -B assay has
significant cross-reactivity with neuropeptide Y (NPY),
-melanocyte-stimulating hormone, or other major neuropeptides, and the
orexin-A and -B assays do not have significant cross-reactivity with each
other. The orexin-A antiserum does not cross-react with mouse hypocretin-1,
whereas the cross-reactivity of orexin-B anti-serum with mouse hypocretin-2 is
100%. The within-assay coefficient of variation for orexin-A was 5.0% and for
orexin-B 6.3%.
Statistical analyses. Data were expressed as means ± SE and analyzed by two-way analysis of variance followed by Student's t test.
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RESULTS |
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Orexin and Fos immunoreactivities in LHA neurons. Orexin-immunoreactive cell bodies were seen in the LHA, confined to the peri- and subfornical distribution previously reported (20,21,22,23,24). The cell bodies were spheroidal or elongated, and orexin-immunoreactive fibers were found in the PVN, LHA, ARC, and median eminence. Numbers of cell bodies immunoreactive for orexin were not different among euglycemic rats and hypoglycemic rats, either fasted or freely fed (Fig. 1).
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Fos distribution induced by hypoglycemia was the same as in a previous report (28). In euglycemic rats, Fos immunoreactivity (confined to the nucleus) was seen in occasional cells of the thalamus and hypothalamusnotably in the PVN, LHA, DMN, and ARC (Fig. 2). In hypoglycemic fed rats, numbers of Fos-immunoreactive neurons in the LHA were slightly but not significantly higher than in vehicle-treated controls, whereas the hypoglycemic fasted rats had significantly more Fos+ nuclei than both other groups, with nearly twice as many as in controls (P < 0.001) (Table 2, Figs. 1 and 2).
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Double-labeling showed that only sparse Fos+ nuclei belonged to orexin neurons in both controls and hypoglycemic fed rats (1.2 and 2.1% of Fos+ nuclei, respectively) and that only 0.7 and 1.7% of orexin cells in control and hypoglycemic fed rats included a Fos+ nucleus. However, coincidence of Fos with orexin was significantly increased in hypoglycemic rats that were not allowed to eat compared with both control and freely fed hypoglycemic rats (Table 2 and Fig. 1). In this group, 8.8% of Fos+ nuclei belonged to orexin neurons, whereas 13.9% of orexin neurons were Fos+.
Fos in the PVN and ARC. Controls and fed hypoglycemic rats had comparable low numbers of Fos+ cells in the ARC (Table 2). In hypoglycemic rats that were fasted, numbers of Fos+ nuclei in the ARC were significantly increased by 2-fold and 1.5-fold compared with those in controls and hypoglycemic fed rats, respectively (both P < 0.05). Similarly, Fos+ cells in the PVN were increased by 1.5-fold and 2-fold, respectively (both P < 0.0001), in hypoglycemic fasted rats compared with the control and hypoglycemic fed groups (Table 2).
Fos in the NTS and DMNX. Neither euglycemic controls nor freely fed hypoglycemic rats showed any Fos+ neurons in the NTS, whereas hypoglycemic fasted rats had abundant Fos+ cells in the NTS. Fos+ nuclei were observed in several subregions of the NTS, including the region of the C2 adrenergic cell complex and the ventral portion of the commissural NTS, with a sparser distribution near the border with the area postrema. Fos nuclei were also strikingly abundant in the dorsal motor nucleus of the vagus (DMNX), an area adjacent to the NTS, at a rostro-caudal level extending between 12.8 and 14.1 mm caudal to Bregma (Fig. 3 and Table 3). Labeled cell counts in the brain-stem are shown in Table 3 and Fig. 3.
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Orexin-A and -B concentrations in the hypothalamus. Orexin-A and -B were both detected in all hypothalamic extracts from all rats. In controls, orexin-A levels were approximately twice as high as orexin-B levels (P < 0.0001). Compared with euglycemic rats, orexin-A levels were not changed in hypoglycemic rats, whether freely fed or fasted throughout (Fig. 4). Orexin-B concentrations in hypoglycemic freely fed rats were comparable to those in controls. Notably, however, hypothalamic orexin-B concentrations were raised in the fasted hypoglycemic rats, being over 10-fold higher than those in both other groups (both P < 0.0001) (Fig. 4).
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DISCUSSION |
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Previous studies by our group and others (11,25,34) indicate that prepro-orexin expression in LHA neurons is stimulated by profound hypoglycemia, suggesting that increased release of the appetite-stimulating orexin-A could contribute to hypoglycemia-induced hyperphagia. Interestingly, prepro-orexin expression does not increase if hypoglycemic rats either have free access to food (25) or are given a glucose infusion to maintain euglycemia (34). Prepro-orexin expression also increased in rats fasted for 48 h, but not in others that were food-restricted for several days to produce weight loss comparable to 48 h of starvation or in several other conditions of increased hunger, including insulin-deficient diabetes and provision of a highly palatable diet (25). Here, we demonstrated that activation of orexin neurons was significantly increased 5 h after insulin injection in profoundly hypoglycemic rats that were not allowed to eat, whereas no such increases were found in hypoglycemic rats that were allowed free access to food. These findings parallel our previous studies of prepro-orexin expression and support our suggestion that orexin neurons are stimulated by falling glucose and inhibited by feeding-related signals (25). Prandial signals in the hypoglycemic rats that ate could include the presence of food in the gut (e.g., gastric stretch receptors) or increases in glucose or other nutrients in the hepatic portal circulation.
However, it is not clear whether orexin neurons respond directly to changes in local glucose availability, as is the case for certain neurons in the ventromedial hypothalamus and NTS (10,35), or whether modulating signals are relayed indirectly to the LHA (6). One well-established indirect pathway is the projection ascending from the NTS, which receives input from visceral glucoreceptors and other vagal afferents (36). The NTS contains neurons that are stimulated by falling glucose in the systemic or portal circulations and inhibited by rising systemic or portal glucose and by gastric distension (10,37); these neurons therefore have the potential to integrate various feeding-related signals.
We found significantly increased numbers of activated orexin and nonorexin LHA neurons only in fasted hypoglycemic rats. Neuronal activation in the LHA was accompanied by the appearance of Fos+ neurons in the NTS and adjacent DMNX; these responses were entirely absent in both the euglycemic controls and in the fed hypoglycemic rats. The DMNX, intimately linked with the NTS, controls gut motility and the secretory responses that are particularly important in feeding behaviors (38). The strikingly similar pattern in the NTS and LHA leads us to believe that the signals that triggered orexin neurons may be relayed via the NTS, which has a major projection to the LHA (8).
Interestingly, orexin neurons accounted numerically for only about one-quarter of the additional number of LHA neurons activated in fasted hypoglycemic rats (Table 2). The neurochemical identities of the other Fos+ neurons are not clear. The activation of neurons in the ARC and PVN, both sites involved in regulation of feeding, suggests that some neurons from these areas may contribute to hypoglycemia-induced hyperphagia. Prolonged (24-h) fasting is known to activate neurons in these areas (39,40); however, the brief 5-h fast during the light phase (when the normoglycemic rats ate <1 g) was unlikely to have affected Fos expression per se in the hypoglycemic food-deprived group. An obvious candidate is NPY, the most potent appetite-stimulating peptide known, which is produced in the ARC and released in sites including the PVN and LHA (32). However, hypothalamic NPY gene expression and NPY levels were not affected by hypoglycemia in rats allowed to eat (41,42), although NPY has not been reported in fasted hypoglycemic rats. MCH, produced by a separate population of LHA neurons (32), stimulates feeding experimentally and may regulate energy homeostasis because MCH-deficient mice are hypophagic and lean (43). MCH expression is increased by insulin-induced hypoglycemia, but MCH neurons do not show Fos immunoreactivity during hypoglycemia (44). There are extensive synaptic connections between NPY, proopiomelanocortin, MCH, and orexin neurons (22,45), and functional interactions among these may drive hyperphagia during hypoglycemia.
We found 14% of orexin neurons to be Fos+, a lower
proportion than that reported by others
(11). This finding may reflect
a difference in the time course of hypoglycemia or the methodology used to
demonstrate and localize Fos immunoreactivity. Using this dose of
intermediate-acting insulin (which was selected for its consistent ability to
stimulate feeding), plasma glucose dropped rapidly and remained low from 2 h
after insulin administration; hypoglycemia was more profound in rats that were
fasted throughout than in rats that were freely fed
(25). As with other
immunohistochemical methods, our double-fluorescence technique may not be
sensitive enough to detect all activated orexin neurons, although
false-positive results are less likely using this approach. The Fos method is
widely used to identify and map neuronal activation across relatively large
brain areas, but we acknowledge that it may miss neuronal activation via
pathways other than Fos, as may be the case with MCH
(11).
We previously reported that prepro-orexin mRNA levels were increased twofold during 6 h of hypoglycemia with fastingthe same conditions that activated orexin and other neurons in the LHA and neurons in the NTS/DMNX. In this study, we found that whole hypothalamic concentrations of orexin-B were markedly raised by 10-fold above those for controls in the hypoglycemic fasted group; levels were unchanged in fed hypoglycemic rats, and orexin-A showed no changes in either hypoglycemic group. The increase in orexin-B levels specifically accompanied the other indexes of orexin neuronal activation, but the magnitude of the rise and its dissociation from the unchanged orexin-A levels is difficult to explain or to relate to the physiological and behavioral effects of hypoglycemia. Increased prepro-orexin expression should equally increase the production of orexin-A and -B because both are derived from the common precursor. Because orexin-A levels were not increased, it is possible that orexin-A release is enhanced in parallel with gene expression; increased synaptic availability of orexin-A would be predicted to stimulate feeding (13) and could contribute to the hyperphagia of hypoglycemia. The increase in orexin-B concentrations could be explained by a selective decrease in release, causing this peptide to accumulate within orexin neurons and terminals. Orexin-B has little, if any, effect on feeding (16) and instead may act preferentially on the OX-2 receptor, which is concerned with arousal: a mutation in OX-2 causes narcolepsy in dogs (46), whereas orexin knockout in mice causes disturbed arousal-sleep patterns (47). We therefore speculate that orexin-B release may be blocked in severe hypoglycemia, leading to its accumulation in the hypothalamus, and that this may contribute to the somnolence and ultimately coma induced by hypoglycemia. This discrepancy between orexin-A and -B may indicate that orexin neurons are functionally heterogeneous and that one subpopulation may be involved in mediating feeding (via orexin-A release), whereas another (releasing orexin-B) may regulate sleep and arousal. This explanation must remain speculative until orexin-A and -B release are measured separately in vivo in key hypothalamus regions and also in the locus coeruleus, which receives a dense orexinergic projection and controls the sleep-wake cycle (48).
In conclusion, we found striking activation of orexin and other LHA neurons in hypoglycemic rats that were not allowed to eat, but not if food was freely available. Increased activation of orexin neurons was accompanied by the emergence of Fos+ neurons in the NTS and DMNX, indicating that the projection from the NTS to the LHA may be an important regulator of orexin neurons and their responses to changes in glucose availability and prandial signals. These findings support our suggestions that orexin neurons are some of the glucose-sensitive neurons in the LHA and that they are regulated by visceral signals relayed via the NTS. As such, they may be involved in triggering hunger and eating in response to hypoglycemia and perhaps in terminating feeding episodes. Orexins may be involved in various aspects of the hypothalamic responses to hypoglycemia, and their precise roles deserve further clarification.
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ACKNOWLEDGMENTS |
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We are indebted to Dr. David Cutler for his valuable discussion and input.
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FOOTNOTES |
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3-D, three-dimensional; ARC, arcuate nucleus; DMN, dorsomedial hypothalamic nucleus; DMNX, dorsal motor nucleus of the vagus; LHA, lateral hypothalamic area; MCH, melanin-concentrating hormone; NPY, neuropeptide Y; NTS, nucleus of the solitary tract; PBS, phosphate-buffered saline; PVN, paraventricular nucleus.
Received for publication February 14, 2000 and accepted in revised form September 8, 2000
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REFERENCES |
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