1 Departments of Medicine and
2 Surgery, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden
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ABSTRACT |
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INTRODUCTION |
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We hypothesized that a possible role for muscle lipolysis is to regulate the local supply of energy during caloric restriction. In this condition there is increased lipid utilization and decreased glucose oxidation (10). As a model, we chose to use very lowcalorie diet (VLCD) instead of total fasting. VLCD offers the minimum nutrients needed to avoid the risks of total fasting while inducing effective weight-reduction (11). The major energy source during caloric restriction is stored TGs. The FFAs liberated are subsequently used for lipid oxidation, which mainly occurs in skeletal muscle. However, it has been generally accepted that FFAs derive from lipolysis in adipose tissue during caloric restriction (12).
The aim of this study was to investigate the influence of insulin on lipolysis in muscle and fat tissues and to analyze results in relation to lipid oxidation. We simultaneously monitored adipose tissue and skeletal muscle tissue lipolysis with microdialysis and performed whole-body measurements of energy expenditure and fuel utilization with indirect calorimetry before and at the end of a 2-week VLCD period in obese subjects. The effects of insulin during a two-step euglycemic-hyperinsulinemic clamp were also investigated.
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RESEARCH DESIGN AND METHODS |
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The thickness of the subcutaneous adipose tissue layer over the muscle fascia was determined by ultrasound before insertion (range 7.517 mm), and an intramuscular location of the catheter was confirmed by the development of muscle twitches during insertion. Local skin anesthesia (EMLA; Astra, Södertälje, Sweden) was used before insertion of the catheters. The microdialysis catheters were continuously perfused with Ringers solution (Apoteksbolaget, Umeå, Sweden), containing 147 mmol/l Na, 4 mmol/l K, 2.3 mmol/l Ca, and 156 mmol/l chloride, at 0.3 µl/min using high-precision pumps (CMA/100 microinjection pump; CMA Microdialysis).
In previous studies using the same flow rate and length of microdialysis catheter, a recovery of >90% for glycerol has been shown in both tissues (14), thus indicating that almost the true tissue levels are recorded. Microdialysate samples were collected in continuous 30-min fractions. The 60-min baseline sampling was started 120 min after insertion of the microdialysis catheters to ensure steady-state conditions (14,15). During the investigation, the subjects rested in a recumbent position and at an ambient temperature of 22°C. After placing the subjects head in a ventilated plastic hood, indirect calorimetry measurements (Deltatrac; Datex Instrumentarium, Helsinki, Finland) were performed continuously for 1 h in the basal resting state and then during the last 30-min period of each insulin step. The subjects voided before and after the study period, and the urine was collected for analysis of nitrogen.
After baseline, a two-step euglycemic-hyperinsulinemic clamp was performed. Insulin infusion rates (Actrapid; NovoNordisk, Copenhagen) were 12 and 80 mU/m2 body surface area · min. Before the higher dose, a bolus of 1.6 mU/m2 body surface area · min was given. Euglycemia was maintained by means of a variable glucose infusion (200 mg/ml) and bedside glucose measurements (HemoCue, Ängelholm, Sweden) every 5 min. Plasma samples for the determination of glycerol, glucose, and free insulin were drawn in the middle of each 30-min dialysate sampling period. The tissue and plasma concentrations from the last 30-min period at baseline and at each 90-min insulin step, respectively, were used for calculations.
Tissue blood flow (TBF) was measured using the 133Xe-washout method (16), as previously described in detail (8). For the determination of adipose TBF (ATBF), Xenon (1.0 MBq in 0.1 ml saline; Mallinckrodt, Petten, the Netherlands) was injected into the subcutaneous tissue of the abdomen, contralaterally to the microdialysis catheters, 30 min before the start of the baseline period. After 30 min, the residual activity was continuously recorded by a scintillation detector (Mediscint; Oakfield Instruments, Oxford, U.K.) during the entire study period. The last 30 min of the baseline period and each insulin infusion step were used for blood flow calculations. For determination of skeletal muscle blood flow, repeated injections were necessary because the decay curve becomes multiexponential over time (17). Thus, three separate injections of 0.3 MBq of Xenon dissolved in 0.1 ml saline were carried out in the gastrocnemius muscle. The site for each injection was changed to avoid interference by residual activity. Recordings were started 5 min after the injection and continued during the last 30 min of the baseline period and each insulin step. The first 10 min of the skeletal muscle decay curve was used for calculations. This is the early and supposed linear (17) phase of isotope washout.
In one set of separate methodological experiments on four healthy control subjects, microdialysis catheters were inserted as previously described in adipose tissue and calf muscle (n = 12 for each tissue). The catheters were perfused with Ringers solution with or without Dextran (60 kDa, 3%) (Braun Medical, Solna, Sweden) to exclude an effect of a colloid on sample volume and recovery. After an equilibration period of 60 min, samples were collected every 30 min during 3 h. In another set of separate methodological experiments, basal blood flow measurements in muscle were performed twice, exactly as previously described, with 410 months between the measurements. Seven nonobese subjects participated in this study.
Analytical procedures.
Microdialysate glycerol was determined with an enzymatic fluorometric method, using a tissue sample analyzer (CMA/600; CMA Microdialysis) (5). Plasma glycerol was determined by bioluminescence (18). Plasma free insulin was determined by a commercial radioimmunoassay (Pharmacia, Uppsala, Sweden) after precipitation with polyethyleneglycol (19) and FFAs in serum by an enzymatic colorimetric method (Wako Chemicals, Neuss, Germany). O2 and CO2 concentrations in the gas from the ventilated hood were measured by a paramagnetic analyzer and by an infrared analyzer, respectively. The analyzers were calibrated with air and precisely known gas concentrations (95% O2 and 5% CO2) before each study. Urinary nitrogen was measured by the Kjeldahl method (20). Glucose, hematocrite, O2 saturation, cholesterol, and TGs were analyzed using the routine clinical laboratory.
Calculations.
TBF was calculated according to the following equation:
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where K denotes the rate constant of the decay of the residual activity and represents the tissue to blood partition coefficient (10 ml/g for adipose tissue [16] and 0.7 ml/g for skeletal muscle [21]).
Glycerol release from the tissues was calculated according to Ficks principle:
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Venous (V) glycerol was calculated from interstitial (I) glycerol according to the following equation:
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where V denotes venous glycerol, A arterial glycerol, Q calculated plasma blood flow, and ps the permeability surface product area (adopted to be 5 ml/100 g · min) (22,23).
Average values for respiratory gas exchange were calculated over each 1-min period for O2 consumption, CO2 production, and respiratory quotient (RQ). The nonprotein respiratory quotient (npRQ) was calculated from calorimetric values and urinary nitrogen. Substrate oxidation was obtained with the formulae of Weir (24), which are based on npRQs of 1.00 for 100% carbohydrate oxidation and 0.707 for 100% fat oxidation. Protein oxidation was assumed to be constant during the study period.
Statistics.
Values are means ± SE. Paired comparisons of the two study occasions were performed using Students paired t test or Wilcoxons signed-rank test when applicable. For comparisons over time of a parameter (basal and 1st and 2nd insulin infusions), a one-way repeated measurement analysis of variance (ANOVA) was used. In post hoc analysis, the Scheffes test was considered significant at P < 0.05. Linear regression analysis was also used. Statistical calculations were made using a statistical software package (Stat View II; Abacus Concepts, Berkeley, CA).
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RESULTS |
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The measurements were performed exactly as in the obese study group, with a 4- to 10-month interval between measurements. R values for the decay curve were 0.920.98 for the first investigation and 0.950.98 for the second investigation. Blood flow (ml/100 g · min) was 2.0 ± 0.2 at the first occasion and 1.8 ± 0.2 at the second. These values did not differ significantly. The coefficient of variation for duplicate determinations was 20.6%. Thus, it is calculated that in eight subjects (the size of the present study group), a difference of 0.4 ml/100 g · min in skeletal muscle blood flow within an individual, i.e., 25% of the blood flow at basal conditions, can be detected with 80% power at a 5% significance level.
We also wanted to make certain that glycerol levels in muscle were at steady state during baseline (Fig. 1). Raw data for glycerol concentration in microdialysate of muscle are shown for three typical subjects before and during VLCD. As expected, and confirming earlier observations (14,15), there was a marked decline in muscle glycerol immediately after insertion of the microdialysis catheter. After <120 min, glycerol concentrations in dialysate were constant. We also analyzed the two different baseline samples (30-min period each) in fat and skeletal muscle tissue of all subjects. Almost identical values for the two samples were recorded before and during VLCD in both tissues, so baseline steady-state conditions were at hand.
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Blood flow.
ATBF was similar at baseline before (1.6 ± 0.4 ml · 100 g-1 · min-1) and during (2.0 ± 0.2 ml · 100 g-1 · min-1) VLCD. ATBF did not change significantly during either of the insulin infusion rates, neither before nor during VLCD. Therefore, the mean ATBF for the full study period was used for the subsequent calculations. Basal skeletal muscle tissue blood flow (MTBF) was 0.7 ± 0.3 and 0.4 ± 0.1 ml · 100 g-1 · min-1 (NS) before and during VLCD and did not change significantly during insulin infusion before caloric restriction. However, during VLCD there was a marked increase in MTBF (1.8 ± 0.5 and 1.3 ± 0.3 ml · 100 g-1 · min-1 at the low- and high-insulin infusion rates, respectively (ANOVA F = 7.4, P = 0.006). This represents a three- to fourfold stimulation of skeletal MTBF by insulin. There was no significant difference between the skeletal muscle blood flow at the low- and high-insulin infusion rates, respectively.
Glycerol production.
We used the glycerol values recorded in Fig. 3A to calculate glycerol release according to Ficks principle, because these values, as well as the blood flow rates during insulin infusion, were in steady state. The glycerol release from adipose and skeletal muscle tissue is shown in Fig. 4. The adipose tissue glycerol release decreased during insulin infusion before (P < 0.001) and during (P < 0.001) caloric restriction (Fig. 4A). The rate of glycerol release in the basal state and after the two insulin infusion periods was almost identical before and during VLCD (F = 1.5 by two-way ANOVA, P = 0.2). Insulin reduced the rate of glycerol release in a dose-dependent fashion by 40% in both conditions.
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DISCUSSION |
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In contrast to the findings with whole-body glucose uptake, the action of insulin on fuel oxidation shows clear signs of hormone resistance during VLCD, when the capacity of insulin to stimulate glucose oxidation and inhibit lipid oxidation was markedly reduced. Thus, it appears that the body shifts from using glucose to lipids as an energy source during insulin stimulation in a VLCD situation. This may be the major reason behind the observed loss of body fat during VLCD that occurs despite reduced energy expenditure and decreased carbohydrate oxidation. However, it should be noted that we have overestimated the actual fat loss by the bioimpedance method, as judged by calculations of actual fat loss.
An important question arises from this study: what is the mechanism behind maintenance of a high rate of lipid oxidation during insulin-glucose infusions in VLCD? It is generally accepted that FFAs derived from adipose lipolysis are the only important source for lipid oxidation during caloric restriction (12,28). On the other hand, these ideas about FFAs were conceived before the recent discovery of lipolysis in skeletal muscle. It is not currently possible to directly measure FFA release from tissues. However, direct information on in vivo lipolysis in the two tissues can be obtained by measurements of glycerol release. True rates of lipolysis in the muscle might be slightly underestimated in our subjects because some of the glycerol formed during lipolysis in the tissue could be reutilized (29,30).
As expected, in adipose tissue, insulin inhibited the rate of glycerol release. The lipolysis rate was inhibited to the same extent before and during VLCD (40%). Hence, the cause of resistance in insulin-inhibited lipid oxidation during VLCD cannot be found in adipose tissue lipolysis. It should be stressed that this notion is based on measurements of the subcutaneous fat depot. This is by far the largest fat region in humans, constituting >80% of the total adipose tissue depot. Presently, it is not possible to directly measure lipolysis in vivo in visceral fat regions. Indirect information can be obtained by combining isotopic infusion with selective venous catheterization (31). However, for technical reasons, it was not possible to add these methods to the present experimental protocol. A unique regulation of visceral lipolysis during VLCD seems unlikely, as judged by the findings with circulating glycerol and FFAs, which were decreased by insulin infusion to the same extent before and during VLCD.
If we accept that the maintenance of a high rate of lipid oxidation during insulin-glucose infusions in VLCD cannot readily be explained by FFAs derived from adipose tissue lipolysis, then we must focus our interest on the other possible lipolytic source, namely skeletal muscle. Our study showed that before VLCD, insulin did not influence the rate of glycerol release from skeletal muscle. During VLCD, insulin caused a quite unexpected and marked stimulation of glycerol release. This effect was maximal at the lowest infusion rate (approximately fivefold stimulation of the basal rate), when there was only a moderate increase in circulating insulin. At the moment, we have no molecular mechanism to explain why insulin actually stimulates the rate of lipolysis in skeletal muscle because there are no laboratory animal or human models available for detailed investigations of lipolysis in myocytes or skeletal muscle tissue. Furthermore, it cannot be clearly stated whether the increased skeletal muscle lipolysis results from an increased activity of lipoprotein lipase in the vascular compartment or from a hormone-sensitive lipase in the myocytes.
However, in a recent study we investigated glycerol levels in adipose tissue by the techniques of venous cannulation and microdialysis (32). Lower glycerol levels were recorded with microdialysis than with venous cannulation. This might be caused by the fact that microdialysis mainly reflects glycerol derived from lipolysis of TGs within fat cells, whereas the cannulation technique measures all lipolysis, i.e., from circulating as well as adipocyte TGs. Nevertheless, in accordance with the present data, we have recently shown a lack of lipolysis suppression in skeletal muscle during another hyperinsulinemic condition, namely that of oral glucose loading, in both obese and normal-weight subjects (8). One reason for lack of an antilipolytic effect could be that human skeletal muscle contains very low levels of phosphodiesterase III, and this enzyme does not seem to be functionally active in the muscle in vivo (33). Phosphodiesterase III is believed to be the key enzyme responsible for the antilipolytic effect of insulin (34). Another factor could be the blood flow in skeletal muscle. Before VLCD, insulin did not stimulate skeletal muscle blood flow. On the other hand, insulin infusion during VLCD caused a marked increase in skeletal muscle blood flow. It is possible that this reflects a defective blood flow regulation in the obese subject (35), which is normalized during weight loss. The insulin effects on blood flow and glycerol release were of the same order of magnitude (four- to fivefold), and both were already maximally increased at the lowest infusion rate. It is likely that the increase in glycerol release in response to insulin demonstrated in skeletal muscle during VLCD is secondary to insulin stimulation of blood flow in the tissue. It should also be noted that we have measured net release of glycerol and not total production of glycerol by the muscle.
Because the stimulation of skeletal muscle blood flow during VLCD by insulin is the crux of the study, it is necessary to consider the methodological aspects. We believe that the findings with muscle blood flow are valid for the following reasons. First, the separate methodological study showed that baseline blood flow is stable over time, and the slope of the decay curve is accurate (r always >0.9). In addition, the statistical power calculation revealed that the increase in flow by insulin during VLCD was far above the detection limit for blood flow changes within an individual. Second, we recently compared, using the present experimental protocol, muscle blood flow and glycerol release in muscle from lean and obese women (E.M., E.H-T., J.B., S. Sjöberg, unpublished observations). In lean individuals, insulin infusion stimulates blood flow markedly, whereas this study confirmed that in obese individuals, insulin was ineffective. Third, we have measured skeletal muscle blood flow after an overnight fast in three different groups of obese subjects. The mean blood flow rates were similar in these groups: 0.7 ± 0.3 (in the present study), 0.9 ± 0.3 (for the eight women in reference 8), and 0.7 ± 0.2 ml · 100 g-1 · min-1 (in E.M., E.H-T., J.B., S. Sjöberg, unpublished observations). Based on these data, it is proposed that obese women are resistant to the stimulatory effect of insulin on blood flow in skeletal muscle. During VLCD, this feature of insulin action improves. Because of a yet unidentified mechanism, the hypocaloric state also causes insulin to paradoxically stimulate glycerol release from muscle. Interestingly, in lean subjects, insulin did not stimulate glycerol output from muscle, despite the stimulatory effect on blood flow.
It should be noted that slightly lower hormone levels during hyperinsulinemic infusion were observed during, compared with before, VLCD. Unfortunately, it is not possible to perfectly match insulin levels when infused under two conditions that differ in hormone kinetics. However, this has no bearing on the interpretation of our results. First, lipolysis in adipose tissue was equally suppressed by insulin infusions before and during VLCD. Second, a lack of effect of insulin infusion on skeletal muscle lipolysis was observed before VLCD, although hormone levels during infusion were higher than during VLCD (when insulin stimulated lipolysis).
The current data point to an important methodological issue when using the microdialysis technique for studying tissue glycerol levels. When microdialysis data are interpreted, variations in the TBF must be monitored and accounted for. Skeletal muscle blood flow was not changed by insulin infusion before dieting, a finding which is in accordance with previous studies (14,15). Interstitial muscle glycerol levels were decreased after insulin infusion in agreement with earlier findings (7,14). However, the rate of glycerol release was not influenced by insulin. There are several explanations for this discrepancy. Compartmentalization and/or reutilization of glycerol by muscle could be influenced by insulin. The hormone could also cause a small stimulation in skeletal blood flow, which could not be detected by the Xenon measurements. It has been demonstrated that tissue glycerol levels can be lowered by selective stimulation of blood flow (36). Nevertheless, the present muscle data imply that a decrease in muscle glycerol levels cannot be interpreted as an inhibition of lipolysis without concomitant knowledge regarding tissue blood-flow rates.
In this study, we used previously determined values for the permeability surface area product (22,23). However, there is no reason to believe that these values are influenced by VLCD. Even if this was the case, then only absolute values for rates of glycerol mobilization would be altered. The major novel finding in muscle would still prevail, showing a lack of insulin effect before VLCD and a stimulatory effect during VLCD.
Although the investigations of lipolysis were performed in a single muscle group (gastrocnemius) in a selected group of patients, we propose the following mechanism for regulation of lipid oxidation during VLCD. A high rate of lipid oxidation is maintained, despite a preserved insulin inhibition of adipose tissue lipolysis, because skeletal muscle lipolysis is actually stimulated by insulin during VLCD. This elevates skeletal muscle FFAs and maintains lipid oxidation, although the supply of FFAs from adipose tissue lipolysis is diminished. Muscle lipolysis might also explain the findings with glucose oxidation and circulating FFAs during VLCD. Insulin-stimulated glucose oxidation was decreased during VLCD, which might be secondary to increased local FFA availability in muscle because glucose and FFAs compete as energy substrates in muscle and because glucose storage takes priority over glucose oxidation in the refeeding state (37). It should be stressed that these mechanisms may only operate under relatively short-term periods of VLCD. Other or adaptive effects may be introduced during longer periods of reduced caloric intake.
In summary, this study provides the first demonstration of a possible physiological role of skeletal muscle lipolysis. This process seems to be an important source of fatty acids for lipid oxidation during caloric restriction, at least in obese women.
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ACKNOWLEDGMENTS |
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We acknowledge the excellent technical assistance of Katarina Hertel, Jan Holm, Britt-Marie Leijonhufvud, and Lena Mannström.
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FOOTNOTES |
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Received for publication 30 June 2000 and accepted in revised form 3 April 2001.
ANOVA, analysis of variance; ATBF, adipose tissue blood flow; FFA, free fatty acid; MTBF, muscle TBF; npRQ, nonprotein respiratory quotient; TG, triglyceride; VLCD, very lowcaloric diet.
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REFERENCES |
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