1 CNRS UMR 8542, Ecole normale supérieure, 46 rue dUlm, 75230 Paris Cedex 05, France
2 Université Denis Diderot-Paris VII, UFR de Biologie, 2 place Jussieu, 75005 Paris, France
3 INSERM U-368, Ecole normale supérieure, 46 rue dUlm, 75230 Paris Cedex 05, France
4 Department of Obstetrics and Gynecology, University of Illinois at Chicago College of Medicine and Chicago Area Veterans Healthcare System (West Side Division), Chicago, IL 60612, USA
5 Laboratoire de Physiologie, Hôpital Necker, 149 rue de Sèvres, 75015 Paris, France
6 Department of Medicine, University of Illinois at Chicago College of Medicine and Chicago Area Veterans Healthcare System (West Side Division), Chicago, IL 60612, USA
7 Department of Physiology and Biophysics, University of Illinois at Chicago College of Medicine and Chicago Area Veterans Healthcare System (West Side Division), Chicago, IL 60612, USA
*Author for correspondence (e-mail: prochian{at}wotan.ens.fr)
Accepted 6 June 2002
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SUMMARY |
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Key words: Hoxa5, Liver, Postnatal growth, Winged-helix/Forkhead box, Foxa2, HNF3ß, FKHR, IGFBP1, IGF1, Mouse
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INTRODUCTION |
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According to the somatomedin hypothesis, liver-derived IGF1 is the main mediator of GH functions (Liu and LeRoith, 1999; Mathews et al., 1986
). In line with this hypothesis, GH stimulates the liver production of circulating IGF1 (Mathews et al., 1986
; Roberts et al., 1986
) and injection of IGF1 into GH-deficient animals restores body growth (Guler et al., 1988
). However, in addition to the systemic activity of liver-derived IGF1, GH may have direct effects on target tissues either without IGF1 mediation, or by controlling local IGF1 synthesis at the level of several organs (DErcole et al., 1984
; Lindahl et al., 1987
). The view, that liver-derived circulating IGF1 is not required in postnatal body growth was recently advocated by reports documenting normal growth in transgenic mice in which IGF1 production was specifically disrupted in the liver (Sjogren et al., 1999
; Yakar et al., 1999
). However, although these mouse models confirm that the liver is a major contributor to circulating IGF1, a lack of function of liver IGF1 in normal postnatal growth is difficult to establish because of compensatory mechanisms.
Complex mechanisms control IGF1 bio-availability that involve the regulation of its synthesis and secretion by all producing organs, including the liver, and at least six circulating IGF-binding proteins (IGFBP1-IGFBP6). IGFBPs prolong the half-life of IGFs and play an important role in controlling their availability and effects on target tissues. For example, IGFBP1, which is mainly produced by hepatocytes, inhibits IGF-dependent cellular growth and differentiation in vitro (Lee et al., 1997), as well as in vivo, as transgenic mice over-expressing IGFBP1 show postnatal growth retardation (Gay et al., 1997
; Rajkumar et al., 1995
; Schneider et al., 2000
).
Transcriptional regulators of the homeobox and Forkhead box/winged helix (Fox) families expressed in the liver play central functions in growth control. Mice that lack HNF1ß (Tcf2/) develop a laron type dwarfism (Lee et al., 1998). Postnatal inactivation of the Foxa2/HNF3ß transcription factor does not impair hepatocyte function or overall animal physiology including growth (Sund et al., 2000
), but its overexpression in the liver leads to a striking growth deficit that has been attributed to the upregulation of Igfbp1 (Rausa et al., 2000
). Other Forkhead box transcription factor are thought to play a major role in Igfbp1 expression, especially in response to hormonal and nutritional status, including FKHR (FOXO1a) and FKHR1 (Foxo1), which are (respectively) the liver-expressed human and mouse homologs of C. elegans DAF-16 (Biggs and Cavenee, 2001
; Guo et al., 1999
).
Hoxa5-disrupted mice present posterior homeotic transformations of the cervico-thoracic region, respiratory tract and intestinal maturation defects, but no apparent neural phenotype (Jeannotte et al., 1993; Aubin et al., 1997
; Aubin et al., 1999
). To verify the possible functions of Hoxa5 in the developing nervous system, we produced transgenic mice overexpressing Hoxa5 under Hoxb2 regulatory elements. These mice do not show any obvious neural phenotype, even in rhombomeres 3 and 5, where the transgene is ectopically expressed. However, they show an impaired postnatal growth correlated with a 12-fold upregulation of Igfbp1 and a two-fold downregulation of Igf1 in the liver. Accordingly, transgenic and endogenous Hoxa5 genes are expressed in the liver at postnatal stages (this study) (Mizuta et al., 1996
). Therefore, in addition to their function in endoderm development (Beck et al., 2000
; Grapin-Botton and Melton, 2000
), Hox genes may play a physiological role in mature endoderm derivatives (James and Kazenwadel, 1991
). It is also proposed that growth regulation by Hoxa5 is due to its interaction with Forkhead box transcription factors, in particular FKHR and HNF3ß/Foxa2, two essential regulators of Igfbp1 and IgfI expression, respectively (Guo et al., 1999
; Nolten et al., 1996
; Yeagley et al., 2001
).
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MATERIALS AND METHODS |
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Digoxigenin-labeled Hoxa5 probes were prepared from pKHX13A (mouse Hoxa5-coding sequence cloned in pBluescript, Stratagene), linearized with HindIII (antisense probe) or XbaI (sense probe) and transcribed with T7 (anti-sense) or T3 (sense) RNA polymerase (Boehringer Mannheim). In situ hybridization was performed in toto on E9.5 mice embryo (Wilkinson, 1992).
lacZ expression, northern blots, radioimmunoassays
lacZ expression was analyzed, using X-Gal staining as previously described (Sham et al., 1993), on various organs of B4B2lacZ mice (mice expressing lacZ under the control of the Hoxb-2 regulatory elements used in B4B2A5m mice) fixed by intracardiac perfusion of 4% paraformaldehyde (PFA).
Total RNA from liver or pituitary was extracted (QIAGEN RNeasy kit). Liver (10 µg) or pituitary (0.5 µg) were separated on denaturing agarose gel, transferred on a nylon membrane, fixed and hybridized with 32P-labeled probes as described above. Igf1 mRNA was detected with an EcoRI-PstI fragment from Igf1 cDNA (Roberts et al., 1987), and GH mRNA was detected with an oligonucleotide probe (5'-GTC TCT GAG AAG CAG AAA GCA GCC TGG GCA TTC TG-3' (LeGuellec et al., 1992
) labeled with terminal deoxynucleotidyl transferase (Boehringer, Mannheim). Membranes were exposed and analyzed by phosphoimaging (Fuji), stripped in boiling SDS 0.5% and rehybridized with GAPDH (Fort et al., 1985
) or ribosomal protein S26 (Vincent et al., 1993
) probes for normalization.
In radioimmunoassays (RIA), serum from coagulated blood samples was centrifuged at 1000 g for 15 minutes and stored at 20°C. IGF1 was measured by non equilibrium RIA (Furlanetto et al., 1977) and GH with a commercial RIA kit (Amersham, France). For T3 and T4 RIA, sodium anilino-naphtalene sulfonate and acidic buffer were used as inhibitors of serum protein binding (Rousset et al., 1984
).
RT-PCR, real time PCR and macroarrays
RT-PCR
PolyA+ mRNA were extracted (Ambion Poly-A Pure kit) and treated with 1 U/µg of DNase I (Amp Grade, GibcoBRL). polyA+ RNA (500 ng) were incubated for 1 hour at 45°C with 4 U/µl Superscript II (GibcoBRL), 10 µg/ml oligo-dT15, 500 µM dNTP, 0.4 U/µl RNasin (Promega) and 10 mM DTT in a final volume of 25 µl. PCR reactions (final volume 25 µl) included 1 µl of cDNA with 10 pmol of each primer, 200 µM dNTP, 1.5 mM MgCl2, 1 U Taq DNA polymerase (Eurobio) in 1xTaq buffer. Amplification conditions were 1 minutes at 92°C, 45 seconds at annealing temperature, 45 seconds at 72°C for 35 cycles; annealing temperature was 55°C for CR5-CR6 and CR7-CR8 couples of primers and 58°C for CR3-CR4. Negative control reactions were obtained in the absence of Superscript. PCR products were analyzed on 2% NuSieve 3:1 agarose gel (FMC).
CR3: 5'-CCA AAT GGC CCG GAC TAC CAG-3'
CR4: 5'-TGC TGC TGA TGT GGG TGC TGC-3'
CR5: 5'-CTG AGA TAA GTT TGT GTT CG-3'
CR6: 5'-TGC ACA TTA GTC ACG ACA AT-3'
CR7: 5'-TGC CGC GGC CAT ACT CAT-3'
CR8: 5'-CCA CTT CAA CCG CTA CCT-3'
Quantitative RT-PCR
Competitor RNA was obtained by inserting a 50 base-pair oligonucleotide in the ApaI site of pSP9A5m (between CR6 and CR7) followed by in vitro transcription using SP6 RNA polymerase (riboMAX, Promega). In competition experiments, 470 ng of liver polyA RNA were mixed with serial dilutions of control RNA. CR6 and CR7 primers were used (2.5 µl of cDNA and 3% NuSieve 3:1 agarose gel).
Real time PCR
SYBR green PCR Core Kit (P/N 4304886, Perkin Elmer Applied Biosystems) was used and the experiment performed according to manufacturers instructions. In brief, 2 µg of total RNA were treated with DNAse I and reverse transcribed in a total volume of 20 µl. Real time PCR was performed, in triplicate, using 1 µl cDNA in a GeneAmp5700 (Applied Biosystems). Primers were:
5'-CGCCACGAGCACCTTGTTCA-3',
5'-TCCTCTGTCATCTCTGGGCTCTCA-3' (IGFBP1) and
5'-TGACGTGCCGCCTGGAGAAAC-3',
5'-CCGGCATCGAAGGTGGAAGAGT-3' (GAPDH).
Macro array analysis
Atlas Mouse 1.2 array (Clontech laboratory, Palo Alto, CA), including 1176 mouse cDNAs, nine mouse housekeeping control cDNAs with reagents for synthesizing radioactive cDNA probes was used in these experiments. RNA from transgenic and control livers were purified using Nucleospin RNAII kit (Clontech). Total RNA (30 µg) was processed according to the instructions of the manufacturer. Results were analyzed with AtlasImage1.5 (Clontech) and normalized by global standardization.
Cell cultures and transfection assays
HepG2 human hepatoma cell line was cultured in F12 Coons modified medium supplemented with 5% fetal bovine serum. Human fibroblast cells (HuF) were isolated from decidua parietalis dissected from the placental membranes after normal vaginal delivery at term as previously described (Strakova et al., 2000) and grown in RPMI-1640 (Invitrogen).
IGFBP1 promoter activation was analyzed using the BP1.Luc (320 pb fragment 5' of the RNA cap site) (Guo et al., 1999). HepG2 and HuF cells were transfected using FuGENE 6 (Roche) or Lipofectamine 2000 (Invitrogen), respectively, as follows. Cells grown in 12-well plates were transfected with 1 µg/well of the IGFBP1 promoter-reporter construct with or without 0.5 µg/well pHA-FKHR (HA-FKHR cloned into XbaI/AccI sites within pALTER) and/or 0.5 µg/well pCL9A5m (Hoxa5m expressing vector) (Montesinos et al., 2001
) expression vectors with the appropriate empty vectors. Twenty-four hours after electroporation, cultured cells were lysed and assayed for luciferase activity (Ausubel et al., 1995
). All transfections were carried out in duplicate or triplicate, and experiments were repeated at least three times.
In vitro and ex vivo protein interactions
GST-Hoxa5 expressing vector (pGA5m) was constructed by inserting in pGEX1 (Pharmacia) the Hoxa5 sequence between GST and Myc tag sequences. GST and GST-Hoxa5 proteins were prepared from E. coli strain TG1. Quantities and size-integrity of fusion-proteins were estimated with SYPRO staining (BIORAD) using BSA as a standard. Binding was performed in a final volume of 200 µl of binding buffer 1 (BF1: 20 mM Tris-HCl pH 7, 100 mM NaCl, 1 mM EDTA, 10% glycerol, 0.01% Nonidet P-40) by incubating 500 ng of glutathione-immobilized fusion proteins with 1 µl of 35S-labeled FKHR, HNF3ß or luciferase synthesized by in vitro transcription-translation (rabbit reticulocyte lysates, TNT, Promega) using pHA-FKHR, HNF3ß cDNA cloned in pMT21 vector (a gift from Dr A. Ruiz i Altaba) and a luciferase construct (Promega). Glutathione sepharose beads (Pharmacia Biotech, 10 µl) were added to the supernatants, rotated overnight at 4°C, rinsed twice with 1 ml of BF1-200 mM NaCl, once with 1 ml BF1-500 mM NaCl and boiled for 5 minutes in Laemmli buffer before analysis by SDS-PAGE and autoradiography.
For ex vivo protein-protein interaction analyses, pEBG or pEBG-FKHR expressing GST native or fusion proteins in eucaryotic cells (Chen et al., 2002) were co-electroporated with pCL9A5m in COS cells (20 µg of each plasmid per 4,000,000 cells). Twenty-four hours later, cells were scraped in binding buffer 2 (BF2: 400 mM KCl, 20 mM Tris-HCl pH 7.5, 20% glycerol, 5 mM DTT, 1% Triton-X100 plus protease inhibitors), frozen in liquid nitrogen, thawed and centrifuged at 4000 g for 15 minutes at 4°C. Glutathione-sepharose beads were added to the cell extracts and treated as described above. Bound proteins were analyzed by SDS/PAGE and western blot, using either an anti-GST (Amersham Pharmacia biotech) or anti-Myc antibody (9E10 monoclonal antibody) (Evan et al., 1985
) to detect GST fusions and Hoxa5m, respectively.
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RESULTS |
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Heterozygous B4B2A5m mice obtained by mating heterozygous transgenic males with wild-type females show no obvious abnormality at birth. However, compared with their sex-matched wild-type siblings, they are smaller after a few weeks of postnatal development (Fig. 2A,B). The growth of transgenic mice of both sexes, normal during the first postnatal week, progressively slows down until the end of the third week (when maximum weight difference is observed) and then resumes normally (Fig. 2C). In many cases, transient arrests of growth and even weight losses were observed between the second and third weeks. No full recovery was ever obtained, the weight of the transgenic animals remaining about 20% inferior to that of wild-type siblings, even after 3 months.
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Hormonal status of B4B2A5m mice: decreased expression of hepatic IGF1
As shown by the ß-galactosidase (ß-gal) staining of Fig. 3AB (B4B2lacZ mice), the B4B2 regulatory elements regulating Hoxa5m expression in B4B2A5 mice are active in the pituitary and thyroid glands. The levels of GH and thyroid hormones in B4B2A5m mice were therefore investigated. Western blots and immunohistochemistry (data not shown) or northern blots (Fig. 3C) failed to demonstrate any significant difference in the pituitary contents of GH protein and mRNA between transgenic and wild-type animals. RIA measurements of circulating GH performed in 24-day-old mice (when maximum differences of weight are observed) showed no modification of GH concentration in transgenic offspring of both sexes (Fig. 3D). Concerning the pituitary-thyroid hormone axis, no differences were found in the concentration of circulating total T3 and T4 thyroid hormones between transgenic and wild-type mice of either sex (Fig. 3E).
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Altered gene expression in the liver of B4B2A5m transgenic mice
Hoxa5 overexpression in the liver of transgenic mice correlates with a downregulation of Igf1 expression. To identify other modifications in gene expression, a global analysis was performed using macroarrays on mRNA from 24-day-old mice. Only a few mRNAs showed different expression levels between transgenic and control livers (examples in Fig. 6A). The decrease in Igf1 mRNA in transgenic animals was confirmed (ratio between control and transgenic: R=0.58) and several other mRNAs were slightly downregulated, for example, ESP1 (Enhancer of split 1 related to Drosophila Groucho gene, R=0.47) and albumin D box-binding protein (R=0.56). By contrast, prothymosin alpha and clusterin-apoJ mRNAs were upregulated (R=2.26 and 3.45, respectively).
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Taken together, these results strongly suggest that the dwarfism of B4B2A5m mice is primarily due to an upregulation of Igfbp1 and, secondarily, to a downregulation of Igf1 in transgenic hepatocytes.
Hoxa5 modulates the FKHR-dependent activation of IGFBP1 promoter
A potent activator of Igfbp1 expression in hepatocytes is the Forkhead box FKHR transcription factor. In its non-phosphorylated form, FKHR activates Igfbp1 transcription through direct binding to FKHR-specific binding sites (Guo et al., 1999; Rena et al., 1999
; Tomizawa et al., 2000
). We thus used a cell transfection assay to investigate the ability of Hoxa5 to modulate the Igfbp1 promoter activity. In the human hepatocyte cell line HepG2, whereas Hoxa5 alone has no significant effect on Igfbp1 promoter, it reduces its activation by FKHR (Fig. 7A). In primary cultures of human fibroblasts (HuF cells), Hoxa5 and FKHR activate the IGFBP1 promoter when expressed separately, and their co-expression results in a dramatic enhancement of the promoter response (Fig. 7B). Taken together these experiments demonstrate that Hoxa5 and FKHR cooperate at the transcriptional level and that the sense and intensity of this cooperation is context dependent.
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DISCUSSION |
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Growth retardation starts at the end of the first week of life. This is slightly earlier than in GH-deficient Snell, Ames and lit mutants, which are indistinguishable from wild-type littermates for the first two weeks (reviewed by Efstratiadis, 1998). The possibility that Hoxa5m expression in the pituitary could explain the dwarfism of B4B2A5m mice was explored. The levels of GH mRNA and protein in pituitary extracts and of circulating GH are identical in sibling transgenic and wild-type mice. These data are in sharp contrast with those obtained from other dwarf mice with an impaired GH system (Li et al., 1990
; Lin et al., 1993
) (reviewed by Voss and Rosenfeld, 1992
) suggesting that growth retardation in B4B2A5m mice is GH independent.
Postnatal growth is also regulated by thyroid hormones. Inactivating the thyroid hormone T3R receptor gene results in hypothyroidism, reduced levels of T3/T4 and total growth arrest after two weeks of life and death (Fraichard et al., 1997
). Phenotypic similarities with B4B2A5m animals pointed towards a pituitary-thyroid axis dysfunction as a possible origin of the dwarfism. However, T3/T4 plasma concentrations are similar in B4B2A5m and wild-type siblings, thus precluding any significant alteration of the pituitary-thyroid axis physiology as a cause B4B2A5m dwarfism.
Postnatal growth defect of B4B2A5m mice probably originates in the liver
The respective 50% decrease and 12-fold increase in liver Igf1 and Igfbp1 mRNAs suggest that the origin of B4B2A5m dwarfism is in the liver. It has been shown that overexpressing IGFBP1 in the liver leads to postnatal growth retardation (Gay et al., 1997; Rajkumar et al., 1995
). In another transgenic mouse model, it was observed that overexpressing FoxA2/HNF3ß in the liver upregulates Igfbp1 expression and induces growth retardation (Rausa et al., 2000
). These phenotypes support the idea that body growth is negatively correlated with IGFBP1 expression and plasma concentration, and that IGFBP1 functions to inhibit IGF1 actions in vivo, most probably by decreasing its bio-availability (Lee et al., 1997
).
Recent studies in transgenic mice have shown that postnatal growth was not impaired when targeted disruption of Igf1 was performed in the liver, even though circulating levels of IGF1 were reduced by 80% (Sjogren et al., 1999; Yakar et al., 1999
). However, mice produced by Yakar and colleagues also display an increase in circulating GH and insulin levels, resulting in a decrease in IGFBP1 and IGFBP2 and in normal levels of free IGF1 (Yakar et al., 2001
). Thus, B4B2A5m mice growth retardation is probably due to the 12-fold increase in Igfbp1 expression that reduces the bio-available fraction of circulating IGF1, independently of its sites of synthesis in the organism and not, or not only, to the 50% decrease in liver Igf1 transcription.
Igf1 and Igfbp1 are expressed in hepatocytes and their up- and downregulation have been observed in the liver. It is therefore tempting to propose that the observed phenotype reflects a cell-autonomous activity of Hoxa5 or of its paralogs in hepatocytes. Indeed, we confirm that Hoxa5 is normally expressed in the liver (Mizuta et al., 1996) and in human-derived hepatocyte HepG2 cells (this report). In B4B2A5m mice, Hoxa5m transgene expression results in a twofold increase in total Hoxa5 mRNA levels in the liver. Expression of the transgene in the liver could be driven by low levels of Egr2 or by other transcription factors capable of binding the Hoxb2 enhancer, as is probably the case for somitic expression.
Hoxa5 and Forkhead box transcription factors interact to regulate liver genes playing important roles in postnatal growth
Forkhead box transcription factors are key regulators of several liver genes playing important roles in postnatal growth, including IGF1 and IGFBP1. FoxA2/HNF3ß is an activator of the P1 promoter of the IGF1 gene, the most active promoter of this gene in liver (Nolten et al., 1996). FKHR, in its non-phosphorylated form, is a strong activator of Igfbp1 transcription through direct binding to FKHR-specific binding sites. FKHR transcriptional activity is negatively regulated by insulin, which provokes its phosphorylation at several sites, leading to its exclusion from the nucleus (Brunet et al., 2001
; Guo et al., 1999
; Rena et al., 1999
; Tomizawa et al., 2000
).
In absence of a change in FKHR transcription in B4B2A5 mice (assessed by real time PCR, not shown), it was thus tempting to address the possibility that Hoxa5 could either regulate, or interfere with, Igfbp1 expression. We first addressed this issue in the human HepG2 hepatocyte cell line, using the human Igfbp1 promoter. Our data indicate that although Hoxa5 by itself is devoid of regulatory activity on this promoter in this cell context, it significantly inhibits its activation by FKHR. This piece of data is at odd with the dramatic overexpression of IGFBP1 observed in the transgenic mice analyzed in the present work. A likely explanation is that the cellular context, i.e. the presence or absence of particular co-factors and/or transcription factors, differs between hepatoma HepG2 cells and the developing liver. This interpretation is comforted by the strong synergy between Hoxa5 and FKHR in another human cellular system (the HuF primary cultures). In any event the two sets of experiments demonstrate without ambiguity that the two transcription factors cooperate to regulate Igfbp1 promoter expression and that this cooperation is context dependent.
Our results are in line with the observations that Homeoproteins and Forkhead box transcription factors interact to regulate target gene transcription (Filosa et al., 1997; Perea-Gomez et al., 1999
). Of particular interest, Pdx1 acts with FoxA2/HNF3ß to regulate its own transcription (Marshak et al., 2000
) and Oct-4 almost completely inhibits the FoxD3-dependent activation of the FoxA2/HNF3ß promoter (Guo et al., 2002
). In a similar way, Engrailed modulates the FoxA2/HNF3ß-dependent regulation of the MAP1B promoter, an effect possibly caused by an interaction between both transcription factors on particular homeoprotein-Forkhead box (HF) DNA-binding sites identified within the MAP1B promoter sequence (I. F., A. P. and A. T., unpublished). In all cases, as for the FKHR-Hoxa5 interaction documented in the present work, a physical interaction between the homeoprotein and the Forkhead box transcription factor has been demonstrated.
In conclusion, it can be suggested that, through FKHR and its homologs (i.e. Daf-16 in C. elegans), homeoproteins play a role in the regulation of growth and longevity (Lin et al., 1997; Ogg et al., 1997
) and that, in addition to their well-established function during development, they have more systemic and physiological roles at late developmental stages and throughout adulthood.
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ACKNOWLEDGMENTS |
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