1 Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and
Development, The Rockefeller University, New York, NY 10021, USA
2 Department of Developmental Biology, Sloan Kettering Memorial Institute, New
York, NY 10021, USA
* Author for correspondence (e-mail: fuchs1b{at}rockefeller.edu)
Received for publication 15 October 2003.
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SUMMARY |
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Key words: Wnt, Gastrulation, Tcf3, Node, Axis
![]() |
Introduction |
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Tcf/Lef proteins can also function to repress the transcription of target
genes (Brannon et al., 1999;
Brantjes et al., 2001
).
Although poorly documented in mammals because of the lack of bona fide targets
of repression, Tcf/Lef proteins can physically interact with co-repressor
proteins such as CtBP and Groucho (Roose
et al., 1998
). In some cases, Wnt signaling has been shown to
reverse repression. Whether Tcf/Lef repression can be alleviated by non-Wnt
mechanisms is unknown.
The human and mouse genomes each contain four Tcf genes, which are
differentially expressed but encode proteins with highly homologous DNA
binding domains and ß-catenin interaction domains
(Korinek et al., 1998b;
Travis et al., 1991
;
van de Wetering et al., 1991
).
Although a double knockout of Lef1 and Tcf1 indicates that
some contexts allow certain Tcfs to share a degree of functional redundancy
(Galceran et al., 1999
),
different Lef/Tcf family members do not always behave similarly when expressed
in the same cell type. Indeed, in mouse skin Lef1 appears to function with
ß-catenin to activate genes involved in hair cell differentiation
(Gat et al., 1998
;
van Genderen et al., 1994
;
Zhou et al., 1995
), but when
transgenically expressed in the same cells, Tcf3 appears to act as a repressor
to specify an alternative cell fate
(Merrill et al., 2001
).
In the developing mouse embryo, anteroposterior (AP) axis formation
initiates during gastrulation beginning at embryonic day 6.5 (E6.5), when
ectodermal cells acquire different fates: at the posterior
embryonic/extra-embryonic border (EEX), an epithelial-mesenchymal transition
occurs to form the mesoderm germ layer at the primitive streak region. The
primitive streak expands distally, and a special group of cells at the
anterior primitive streak (APS) form the axial mesoderm, which gives rise to
the embryonic organizer, i.e. node, which is both necessary and sufficient to
induce the AP axis (Beddington,
1994). Analogous embryonic organizers in other animals are also
necessary and sufficient to induce the primary embryo axis, either the AP axis
(Hensen's node in avians) or the DV axis (Spemann's organizer in amphibians,
the embryonic shield in fish) (Beddington,
1994
; Harland and Gerhart,
1997
; Hensen,
1876
). Further patterning within the mesoderm yields other,
non-axial populations (e.g. lateral and paraxial mesoderm), which produce
somites and other mesodermal structures.
In mouse, Wnt3 is expressed at the appropriate time and location
to promote primitive streak induction, and Wnt3-/- embryos
fail to undergo gastrulation (Liu et al.,
1999). Similarly, ß-catenin-null embryos also fail to specify
an AP axis (Huelsken et al.,
2000
). Ectopic activation of Wnt signaling in early mouse embryos
either by mutations affecting Axin or Apc, negative
regulators of ß-catenin stability, or by transgenic expression of Wnt8c
all lead to ectopic AP axis specification and formation of multiple nodes
(Ishikawa et al., 2003
;
Popperl et al., 1997
;
Zeng et al., 1997
). Thus, Wnt
signaling appears to be both necessary and, in some circumstances, sufficient
for AP axis specification and formation of the node in developing mouse
embryos.
Despite the proven requirement for upstream members of the Wnt signaling
pathway, the role of specific Lef/Tcf proteins in mouse AP axis and node
formation has remained unclear. As judged by gene targeting, ablation of
Tcf1, Tcf4 or Lef1 results in either viable or neonatal
lethal pups, without consequence to early embryonic development. The
Tcf1-/- Lef1-/- double knockout
embryos form excess neural ectoderm at the expense of paraxial mesoderm, as
well as multiple neural tubes in the tail
(Galceran et al., 1999).
Although the Tcf3 locus has not yet been targeted for mutation in any
organism, in zebrafish morpholino knockdown of both Tcf3 homologs
(Hdl and Tcf3b) results in postgastrulation defects in
neural patterning and anterior neural truncations
(Dorsky et al., 2003
;
Kim et al., 2000
). All of
these effects in the Tcf1-/- Lef1-/-
mice and the hdl-tcf3b- zebrafish, however, occur after the induction
of the primary embryonic axis and after the formation of the embryonic
organizer has already occurred. Interestingly, the antisense RNA-mediated
knockdown of maternal and zygotic Xenopus XTcf3 leads to a markedly different
phenotype characterized by the expansion of organizer cell fates and
dorsoanteriorization of embryos (Houston
et al., 2002
). However, a similar role for mammalian Lef/Tcf
proteins in either organizer formation or axis induction has yet to be
identified.
Taken together, the findings to date suggest that either (1) additional
Lef/Tcf functional redundancy accounts for the role of Wnt signaling in axis
and node specification, (2) mouse Lef/Tcf proteins do not play a role in this
process as they appear to do in Xenopus or (3) Tcf3, the lone Lef/Tcf family
member left to be targeted in mice, is the crucial Lef/Tcf member in this
process. Interestingly, Tcf3 is expressed throughout the mouse embryo at E6.5,
prior to primitive streak formation
(Korinek et al., 1998b), which
makes Tcf3 a prime candidate to be either a positive or a negative regulator
of Wnt-mediated AP axis specification. We have now tested this hypothesis
directly by generating a null mutation in the murine Tcf3 gene, and
examining the consequences to Wnt signaling and early mouse embryogenesis. Our
results reveal an essential and unique role for mouse Tcf3 in restricting AP
axis induction during the onset of gastrulation. Similar to its
Xenopus and zebrafish homologs, mouse Tcf3 appears to function by
repressing target genes in the early embryo.
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Materials and methods |
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Embryo in situ hybridization, immunofluorescence and histology
The age of embryos was determined based on the time of day harvested
assuming noon on the day of plug discovery corresponds to day 0.5. For
E6.5-E7.5 embryos staging criteria described
(Downs and Davies, 1993) were
used to determine the stage of gastrulation of embryos.
In situ hybridization was performed essentially as described
(Wilkinson and Nieto, 1993).
Briefly, embryos were fixed overnight in 4% PFA at 4°C, dehydrated in a
graded methanol series and stored at -20°C in 100% methanol until in situ
hybridization was performed. Embryos were then rehydrated, bleached in
methanol/H2O2 (4:1) for 1 hour, washed in PBT, treated
with proteinase K (2-3 minutes E7.5, 4-5 minutes E8.5), post-fixed in
4%PFA/0.2% glutaraldehyde, and hybridized with digoxigenin-labeled cRNA
probes. Hybridized cRNA probes were detected with sheep anti-DIG AP FAB
antibody (Roche). After BCIP/NBT (Roche) reaction to detect signal, embryos
were dehydrated through a graded methanol series to develop the purple colored
precipitate, rehydrated and cleared in 50% glycerol prior to imaging. Embryos
were identified as Tcf3+ or Tcf3-/-
after imaging by digesting embryos in proteinase K and PCR as described above.
Probes used in this study were specific for Hesx1 (P. Thomas),
En1 (A. Joyner), Krox20 (Egr2 - Mouse Genome
Informatics) (D. Wilkinson), Six3 (G. Oliver), Foxa2 (E.
DeRobertis) and brachyury (D.Wilkinson).
Immunofluorescent detection of proteins was performed on PFA-fixed, frozen
sections as described previously (Merrill
et al., 2001) with the following antibodies: guinea pig anti-Tcf3
(Merrill et al., 2001
), mouse
anti-ß-catenin (Sigma) and rat anti-E-cadherin (M. Takeichi). Secondary
antibodies, FITC or Texas Red-conjugated donkey antibodies (Jackson Labs) were
used at 1:200 dilution.
Scanning electron microscopy (SEM)
SEM procedures were performed essentially as described
(Sulik et al., 1994). Embryos
were fixed overnight at 4°C in 2.5% glutaraldehyde and post-fixed in 2%
osmium tetroxide for 2 hours. They were dehydrated in a graded series of
ethanol. Crucial point drying and sputter coating with palladium/gold was used
to preserve structures and highlight surface features. Embryos were mounted on
metal stubs exposing the ventral/distal surface for optimal viewing of the
node. Imaging was performed with a JEOL microscope.
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Results |
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To address this issue, we first assessed whether Tcf/Lef/ß-catenin
regulated target genes are activated in the early mouse embryo with the TOPGal
transgenic reporter, which is responsive specifically to
Tcf/Lef/ß-catenin complexes (DasGupta
and Fuchs, 1999). The first signs of TOPGal activity were seen at
E6.5-E7.0, where X-Gal staining was detected beginning at the early streak
(ES) stages of gastrulation [Fig.
1; for details of staging criteria for gastrulating embryos, see
Downs and Davies (Downs and Davies,
1993
)]. ß-Galactosidase activity concentrated along the
forming primitive streak (ps) region extending along both sides of the
posterior EEX border (Fig. 1A)
and was subsequently expanded distally in late streak (LS;
Fig. 1B) and neural fold stages
(Fig. 1C,D). These results were
consistent with those previously reported for the BAT-Gal Wnt reporter
transgene (Maretto et al.,
2003
) Sagittal sections of TOPGal stained neural fold stage
embryos revealed activity in ectodermal and mesodermal cell types in the
primitive streak region (Fig.
1E) and activity in the posterior half of the ventral node
(Fig. 1E').
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As mesoderm formed at the primitive streak region, the pattern of Tcf3 expression changed dramatically (Fig. 1G-I). Tcf3 immunoreactivity was weak or absent in the primitive streak, while it became intense in the ectoderm and mesoderm anterior to the node. (Fig. 1G). By E7.5, Tcf3 was also detected in a portion of the anterior endoderm. Thus, whereas the E6.5 embryo displayed relatively uniform anti-Tcf3 staining, the E7.5 embryo displayed an anterior gradient of Tcf3 immunoreactivity, with the transition at or near the node (Fig. 1I). Overall, reduction of Tcf3 protein in the posterior of the E7.5 embryos preceded the activation of TOPGal. Intriguingly, the earliest activation of TOPGal at E6.5 preceded Tcf3 downregulation.
Targeted ablation of Tcf3 in ES cells and in mice
If Tcf3 functions prior to gastrulation, then it might be expected to act
in concert with early Wnt signaling to affect the formation and patterning of
the primitive streak. If, however, the function of Tcf3 does not occur until
after the formation of the AP axis, then based on the retention of Tcf3 in the
anterior of the gastrula, Tcf3 might be expected to act in patterning the
anterior of the embryo, similar to the functions previously revealed for
Tcf3b and Hdl (a truncated Tcf3 gene) in zebrafish
development.
To distinguish between these possibilities, we created a null mutation in
the Tcf3 gene in mice. The 64 bp exon 2 of the Tcf3 gene was
targeted for excision, so that if the resulting Tcf3 mRNA lacking
exon 2 were stable, it would contain a frameshift and an early termination
codon. Such nonsense-mutation containing mRNAs are typically unstable and
rarely translated into protein (Wilkinson
and Shyu, 2002). Moreover, previous mice containing potential
truncation mutations residing much further downstream in the Tcf1,
Tcf4 and Lef1 genes have not caused dominant negative effects
(Korinek et al., 1998a
;
van Genderen et al., 1994
;
Verbeek et al., 1995
).
The targeting vector was designed to provide flexibility in engineering either embryonic stem (ES) cells lacking exon 2, or ES cells harboring a floxed exon 2 for conditional knockouts (Fig. 2A). For the present study, we focused on the straight knockout. Shown in Fig. 2B are representative examples of the Southern blot analyses of ES cell genomic DNA, revealing a 5.9 kb HinCII fragment diagnostic for the floxed PGK-Neo cassette between exons 2 and 3 of the Tcf3 gene, and a 3.3 kb BglII fragment revealing the presence of the loxP site and new BglII site inserted between exons 1 and 2 of the Tcf3 gene (+/neo). After transfection with CMV-Cre plasmid, the loss of the PGK-Neo cassette was confirmed by Southern blot (+/in Fig. 2B), and PCR verified the loss of the 170 bp exon 2 fragment (Fig. 2C).
From 26 litters involving two Tcf3+/- heterozygote parents, 60 mice (36%) were Tcf3 +/+ and 104 mice (64%) were Tcf3+/-, as judged by PCR analyses (data not shown). These animals appeared normal, indicating no dominant-negative or haploinsufficiency defects from the single Tcf3 allele alteration. However, between newborn and E11.5, no intact Tcf3-/- embryos were found, suggesting that the loss of Tcf3 resulted in early embryonic lethality.
The first intact Tcf3-/- embryos were recovered from three E9.5 litters (8+/+, 20+/- and 6-/-), although they were only 20-40% the size of wild-type littermates. Although a beating heart and somites were present in some E9.5 Tcf3-/- embryos, anterior neural structures were conspicuously absent (not shown). At E8.5 and earlier, Tcf3-/- embryos were recovered at Mendelian ratios and no signs of excessive resorption were noted. PCR analyses confirmed the existence of the Tcf3 mutation and loss of the Tcf3 wild-type allele (Fig. 2C), and anti-Tcf3 immunoblot analysis confirmed the loss of Tcf3 protein in E8.5 Tcf3-/- embryos (Fig. 2D).
Morphological defects in Tcf3-/-embryos
Just after gastrulation at e8.5, Tcf3+/+ and
+/- embryos (WT) had well-established AP axial structures, distinct
neural-folds, somites and a heart (Fig.
3A,A'). The rostral ends of the neural-folds extend
anteriorly past the developing heart, and forebrain, midbrain and hindbrain
regions are identifiable (Fig.
3A').
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Although supernumerary neural-folds were a hallmark of mildly affected E8.5 Tcf3-/- embryos, these structures were grossly runted or absent in severely affected embryos, which also failed to produce somites and heart (Fig. 3E,F). However, consistent with the presence of multiple neural folds in mildly affected mutants, multiple grooves (ng) along the ventral surface were evident in the severely affected mutants (Fig. 3E). These grooves extended along the anterior half of the embryo, which was frequently bent laterally. Distally, the severely affected embryos frequently exhibited atypically large areas that appeared to lack underlying ectoderm or mesoderm (Fig. 3E,F', red arrows). The significance of these regions is delineated below.
The earliest recognizable defects were detected in E7.5 Tcf3-/- embryos, where a frequent bulging and less frequent duplication of the primitive streak were detected (Fig. 3I). Histological examination of these morphological perturbations revealed an aberrant accumulation of mesoderm (Fig. 3I). Overall, these data were consistent with defects involving partial and sometimes complete duplications of AP axis structures, including neural grooves and primitive streaks. Other abnormalities, including enlarged cardiac sacs, multiple large blood vessels (v) and foregut defects (fg) seemed to be secondary consequences of partial AP axis duplication. We pursue this avenue in greater detail below.
Defective neural patterning in Tcf3-/-embryos
The forebrain defects in postgastrulation E8.5 Tcf3-/-
embryos bore a strong resemblance to those seen upon ectopic activation of Wnt
signaling (Mukhopadhyay et al.,
2001; Popperl et al.,
1997
), and in hdl, tcf3b knock-down zebrafish embryos
(Dorsky et al., 2003
;
Kim et al., 2000
). To explore
the extent to which these later stage defects might resemble artificial Wnt
activation, we examined the expression of neural-specific genes in E8.5
embryos. Fig. 4 illustrates
representative whole-mount in situ hybridization performed on groups of
10 E8.5 embryos (6-9 Tcf3+/+ or +/- and
3-4 Tcf3-/-). Data for +/+ or +/-
embryos were indistinguishable and are referred to as `wild-type'.
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Perturbations were also detected in midbrain and hindbrain gene expression. En1 is typically expressed in neurectoderm at the caudal end of the midbrain (Fig. 4C,C'). In mutant embryos, En1 was expanded rostrally, and its distance from the rostral tip of the embryo was shortened, consistent with the severe reduction of the forebrain (compare bar in Fig. 4C to bar in Fig. 4D). Frontal views revealed that neurectoderm retained En1 expression but did not develop a neural groove (Fig. 4D') like wild-type embryos did (Fig. 4C'). For the hindbrain marker Krox20, wild-type embryos exhibited robust expression in rhombomeres 3 and 5 (Fig. 4E,E'), whereas Krox20 expression in knockout embryos was restricted to a single band on each neural fold, and was markedly reduced in intensity (Fig. 4F,F'). Additionally, rather than being confined to the neural groove, Krox20 was detected on the lateral surfaces of Tcf3 mutant neural-folds (Fig. 4F'). Thus, loss of Tcf3 not only resulted in a failure to specify the most rostral neurectoderm, but also more broadly caused abnormalities in caudal brain regions.
Multiple nodes and notochords in Tcf3-/- embryos
Defects in early patterning events that produced duplications and
expansions of axial structures in Tcf3-null mouse embryos had not
been observed in the morpholino knockdowns of Tcf3 gene expression in
zebrafish. By contrast, gross expansion of Spemann's organizer was observed in
knockdown of XTcf3 from Xenopus embryos, although distinct
duplications of either the organizer or the primary embryo axis in
XTcf3- embryos were not reported
(Houston et al., 2002). Given
the importance of the node and notochord in postgastrulation patterning of
axial structures, the appearance of expanded or duplicated nodes and
notochords in Tcf3-/- embryos could explain many of the
observed morphological defects. Therefore, we next addressed whether the node
and notochord were duplicated and/or expanded in Tcf3-/-
embryos.
Towards the end of gastrulation (E7.5), node and notochord are readily visible by scanning electron microscopy (SEM) as an indented club-like structure on the embryo surface (Fig. 5A). In the normal embryo, the ventrally located node is most obvious, and is composed of small, rounded, monociliated progenitor cells (Fig. 5A,A'; green arrow). Arising anterior of the node, the derivative cells of the notochord are similar in appearance, forming a structure that is two to three cells wide (double blue arrows in Fig. 5A). Both the node and the notochord are readily distinguished from the surrounding, more superficial endodermal cells, which are flatter and larger in appearance.
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To assess whether these node-like and notochord-like structures exhibited molecular characteristics of organizer cell populations, we examined a pair of marker genes, brachyury and Foxa2, which are important for organizer function at this stage. In wild-type embryos, brachyury is expressed in the primitive streak and then concentrates in the node and notochord (ncd) (Fig. 5C,C'). In Tcf3-/- embryos, brachyury was often grossly expanded (Fig. 5D,D'). These patterns varied, but were a direct reflection of the degree of expanded/duplicated node and notochord cells in a given Tcf3-/- embryo. In the most severely affected embryos, brachyury expression nearly covered the entire ventral surface. Similar findings were obtained with Foxa2. In wild-type embryos, Foxa2 is expressed in the node and notochord as well as in the neural floor plate when dorsoventral polarity is established in the neural tube (Fig. 5E,E'). In mutant embryos, Foxa2 expression was considerably broader and variable, but always reflective of the morphological expansions and duplications/multiplications of node and notochord (Fig. 5F,F').
The use of Foxa2 and brachyury as markers in E8.5 embryos provided graphic illustrations of the various different axis defects observed in our Tcf3-/- embryos. The most striking were cases where the axis duplications appeared to occur on opposite sides of the embryo (Fig. 5G,G').
Mesodermal patterning defects in Tcf3-/- gastrulae
To begin to elucidate the causes of the AP axis duplications and expansions
of axial mesendoderm in Tcf3-/- embryos, we examined
molecular events known to be important for proper AP axis induction and
subsequent patterning of the primitive streak mesoderm. The extra-embryonic
organizer, anterior visceral endoderm (AVE), is required early to pattern the
gastrulating epiblast for AP axis formation (for a review, see
Lu et al., 2001). Expressed in
the AVE, the Nodal inhibitors Cer1 and Lefty1
(Lefta - Mouse Genome Informatics) are crucial to this process, as
judged by the AP axis duplications caused by expanded Nodal signaling in
Cer1-/- Lefty1-/- embryos
(Perea-Gomez et al.,
2002
).
To determine whether loss of Tcf3 interferes with the ability of the AVE to restrict Nodal activity, we examined the expression of endodermal markers in Tcf3-/- gastrulae. Cer1 expression was detected in the AVE of all wild-type and Tcf3-/- embryos examined, although it was expanded in the posterior endoderm of the mutant embryos (Fig. 6A-B'). An additional AVE marker, the homeobox gene Hex, was also largely unaffected in its expression (Fig. 6C,D). The intact expression of AVE markers indicated that the axis duplications are not caused by loss of Nodal antagonists in the AVE.
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In E7.0 embryos, Foxa2 is normally expressed in the axial mesoderm
that forms the APS, where it is required for node formation
(Ang and Rossant, 1994;
Weinstein et al., 1994
)
(Fig. 6I). In 75% of the
Tcf3-/- embryos examined, Foxa2 expression was
altered in variable patterns (Fig.
6J,J'). In half of the Tcf3-/- embryos,
Foxa2 expression was clearly expanded but still localized at its
normal position at the distal tip of the primitive streak
(Fig. 6J). In 25% of
Tcf3-/- embryos, signs of ectopic expression were visible
such as shown in Fig.
6J', where strong hybridization was seen along the perimeter
of the extra-embryonic/embryonic border.
Complementary to the axial mesoderm of the anterior primitive streak, the
lateral mesoderm is marked by expression of Lefty2 (Leftb -
Mouse Genome Informatics) (Meno et al.,
1999) (Fig. 6K). Expression of Lefty2 was diminished in most (78%)
Tcf3-/- embryos (Fig.
6L,L'). Concomitant with the reduction in Lefty2
was a corresponding expansion of the region where axial mesoderm normally
develops (area below the red line in Fig.
6K-L'). Together, these findings suggested that the loss of
Tcf3 results in expansion of axial mesoderm at the expense of other mesodermal
cell types.
TOPGal activity retains its expression pattern during gastrulation in Tcf3-/- embryos
Based primarily on the VegT-dependent upregulation of organizer genes in
XTcf3-depleted embryos, a function of XTcf3 in Xenopus gastrulae is
to repress target genes activated by non-ß-catenin dependent mechanisms
(Houston et al., 2002).
Consistent with this model in Xenopus, the removal of Tcf-binding
sites from the Siamois promoter elevates its activity in ventral
blastomeres of embryos (Brannon et al.,
1997
). These data from Xenopus combined with similarities
between Tcf3-/-, Axin-/-,
Apcneo/neo and Wnt8c transgenic phenotypes together
suggest that murine Tcf3 functions to repress target genes during induction of
the AP axis (Ishikawa et al.,
2003
; Popperl et al.,
1997
; Zeng et al.,
1997
).
To evaluate whether Tcf3 mediates its non-redundant effects on early gastrulation through repression or activation of Wnt target genes, we mated the TOPGal transgenic mice on the Tcf3-/- background, and examined TOPGAL expression in gastrulating embryos. If Tcf3 functions as a non-redundant activator of Wnt target genes, TOPGal activity should be absent from the Tcf3-/- gastrula; if Tcf3 functions as a non-redundant repressor that normally counteracts other Lef/Tcf factors in Wnt-receiving cells, then this might be reflected in ectopic TOPGal expression. If the non-redundant role of Tcf3 is to suppress genes that require other factors for their transactivation, then TOPGal should be expressed only in the axial mesodermal cells and their progeny, which are normally TOPGal positive (see Fig. 1).
The results of this experiment are compiled in Fig. 7. TOPGal expression was clearly maintained in the Tcf3-/- gastrula even at the early stages of primitive streak formation (KO; Fig. 7B,C). Analogous to the pattern seen in wild-type embryos, X-gal staining was in the posterior of the embryo, at the EEX border. TOPGal expression was also detected in the primitive streak and node of embryos that had formed neural-folds (Fig. 7D,D'). Interestingly, even in embryos with a clearly duplicated node, TOPGal expression was still faithfully maintained in the correct, but now expanded, cell population (Fig. 7E-F'). As we failed to detect suppression, elevation and ectopic activation of TOPGal, these data best fit a model whereby the non-redundant role of Tcf3 is as a repressor of genes that are not activated by Wnt signaling alone.
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Discussion |
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Studies with XTcf3 knockdowns revealed greater similarities to our mouse
knockout. Knockdown of XTcf3 causes an induction of dorsal blastomere-specific
genes by a VegT-dependent mechanism
(Houston et al., 2002). Many
of the dorsal-specific genes upregulated by loss of XTcf3 are involved in
formation of the Spemann organizer. In mouse Tcf3-/-
embryos, we also find expansions of node cells and the upregulation of
Foxa2, a Nodal target gene required for specification of node cell
types. However, Tcf3-/- mouse embryos displayed distinct
duplications of the AP axis not seen in the Xenopus knockdown
embryos
Curiously, no other Lef/Tcf gene has been directly implicated in AP axis
induction despite the requirement of Wnt3 and ß-catenin for this process
(Huelsken et al., 2000;
Liu et al., 1999
). In mouse,
Lef1-/-, Tcf1-/- and
Lef1-/- Tcf1-/- embryos are all
competent at inducing an AP axis, and Tcf4 is not expressed in pregastrula
embryos (Galceran et al.,
1999
; Korinek et al.,
1998a
; Korinek et al.,
1998b
). Our studies with TOPGal embryos provide evidence that Wnt3
functions through Lef/Tcf-ß-catenin activation of target genes during AP
axis induction. Furthermore, the induction of the AP axis coupled with the
persistence of TOPGal expression in Tcf3 knockout embryos reveals a previously
unrealized level of functional redundancy between Lef/Tcf factors in
transducing this signal.
The putative redundant function of Tcf3 in promoting AP axis induction in Lef1-/- Tcf1-/- embryos becomes particularly interesting when one considers its opposing, non-redundant function in early embryogenesis. The fact that TOPGal activity in Tcf3 mutant embryos is both maintained in the expanded organizer cells and not ectopically expressed in other cells offers important insights into how Tcf3 must be exerting its non-redundant effects. Together, these findings indicate that Tcf3 acts alone in restricting the activation of target genes that are positively regulated by other transcription factors. The simplest model consistent with these data is that the primary function of Tcf3 is essential for repressing the expression of genes that promote AP axis induction and formation of axial mesoderm.
Given the requirement for Nodal signaling during AP axis induction
(Conlon et al., 1994) and axial
mesoderm patterning (Hoodless et al.,
2001
; Vincent et al.,
2003
; Yamamoto et al.,
2001
) and the VegT-XTcf3 interaction elucidated in
Xenopus organizer formation
(Houston et al., 2002
), it is
attractive to speculate that Tcf3 might be repressing transcriptional targets
of Nodal signaling. In support of this model, ectopic primitive streak
formation in chick embryos requires the combined action of both Wnts and a
Nodal-like molecule, Vg1 (Skromne and
Stern, 2001
). As the transcriptional activation of Foxa2
is directly downstream of Nodal signaling
(Hoodless et al., 2001
;
Yamamoto et al., 2001
), the
ectopic expression of Foxa2 in the gastrulating
Tcf3-/- embryo lends further support to this view. We also
noted that a Foxa2 promoter element harboring multiple, conserved
Lef1/Tcf-binding sites directs transgenic expression in axial mesoderm and
node (Sasaki and Hogan,
1996
).
The phenotype of Tcf3 mutant embryos is similar to that reported for the
loss of Axin or Apc, two other inhibitors of Wnt signaling
(Ishikawa et al., 2003;
Popperl et al., 1997
;
Zeng et al., 1997
) and ectopic
expression of Wnt8c (Popperl et al.,
1997
). Previously, these effects were attributed to the ectopic
expression of target genes normally regulated by ß-catenin/Tcf
transactivating complexes. However, the lack of ectopic TOPGal activity in
Tcf3-/- embryos suggests that ß-catenin/Tcf transactivating
complexes are not required for the axis duplication phenotypes. An alternative
mechanism consistent with our data is that Tcf3 functions as a transcriptional
repressor in the absence of ß-catenin, and that ectopic stabilization of
ß-catenin results in relief of Tcf3-mediated repression at sites that are
not reflected here by TOPGal activity. Precedence for a role for
ß-catenin in derepression of Tcf genes has been provided in lower
eukaryotic systems (Cavallo et al.,
1998
).
In conclusion, the potent effects of loss of the repressor function of Tcf3 illustrate the requirement for the coalescence of transcriptional activators and repressors to properly define the temporal and spatial pattern of expression of AP axis inducing gene products. Although in vertebrates, the importance of the switch from repression to de-repression has often been overlooked in comparison with Wnt-mediated Lef/Tcf/ß-catenin activation, this study highlights the importance of Tcf3-mediated repression in shaping cell fate decisions for the establishment of the basic body plan. In the future, identification of target genes that require Tcf-mediated repression will accelerate our understanding of the role of Lef/Tcfs in governing cell fate determination.
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ACKNOWLEDGMENTS |
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REFERENCES |
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