Max-Planck Institut für Entwicklungsbiologie, Abteilung Evolutionsbiologie, Spemannstrasse 37-39, D-72076 Tübingen, Germany
* Present address: Department of Biochemistry and Biophysics, UC San Francisco, San Francisco, CA 94143, USA
Author for correspondence (e-mail: ralf.sommer{at}tuebingen.mpg.de)
Accepted June 18, 2001
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SUMMARY |
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Key words: Evolution, M lineage, Pristionchus pacificus, Vulva, mab-5
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INTRODUCTION |
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At hatching, the gonad consists of four cells, called Z(1-4) and it develops initially in an autonomous fashion. Z(2,3) make the germline, whereas Z(1,4) are the precursors of the somatic gonad. During postembryonic development, Z(1,4) divide multiple times, forming all somatic derivatives of the gonad including the uterus (Kimble and Hirsch, 1979). Early experimental studies on gonad development in C. elegans identified a crucial cell-cell interaction between the gonadal anchor cell (AC) and the underlying epidermis. If the AC was ablated at birth, no vulva was formed, resulting in animals that are egg-laying defective (Kimble, 1981).
The vulva itself is a derivative of the ventral epidermis, which consists of 12 precursor cells, named P(1-12).p according to their anteroposterior position (Fig. 1; Sulston and Horvitz, 1977). The six central cells, P(3-8).p, form a vulva equivalence group, because all these six cells can participate in the formation of the vulva (Sternberg and Horvitz, 1986). However, in wild-type animals only the three cells P(5-7).p form vulval tissue by adopting one of two alternative cell fates. P(5,7).p generate seven progeny each, form the outer part of the vulva and have a so-called 2° fate. P6.p generates eight progeny, forms the inner part of the vulva and has the 1° fate. P(3,4,8).p divide once, remain epidermal and have a 3° fate. A hierarchy of cell fates can be distinguished among P(3-8).p, because cells with a lower fate (i.e. 3° cells) can replace 2° or 1° cells. Similarly, 2° cells can replace the 1° cell, whereas the 1° cell P6.p does not replace any other cell (Kornfeld, 1997; Greenwald, 1997).
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Two different molecular pathways are defined to control the cell-cell interactions of the gonad with other tissues. The AC expresses an EGF-like protein encoded by the gene lin-3, which constitutes the signal for vulva induction (Hill and Sternberg, 1992). Within the VPCs, this signal is transmitted via an EGFR/RAS/MAPK signaling (Kornfeld, 1997). The anterior migration of the SM cells requires EGL-17, an FGF-like protein (Burdine et al., 1997) that is expressed in several cells of the somatic gonad (Branda and Stern, 2000). For the proper formation of the complete egg-laying system, additional reciprocal signaling between the gonad, the SMs and the vulva are required. Once P6.p has been specified to adopt the 1° fate, some of its descendants express the LIN-3 protein themselves and induce a particular cell fate in the uterus (Chang et al., 1999; Newman et al., 2000). Furthermore, P6.p expresses EGL-17 and contributes to the correct alignment of the SM cells (Burdine et al., 1998). Taken together, reciprocal signaling between cells and tissues of different germ layers are required for organogenesis of the egg-laying system.
Cell fate specification and organogenesis can be studied in several free-living nematodes providing insight into the evolution of complex developmental structures (Sommer, 2000). One particularly attractive system for evolutionary studies is the development of the vulva. At the cellular level, multiple differences in cell fate specification and cell-cell interactions have been identified between various nematode species (Fig. 1A) (Sommer, 1997a; Sommer, 2000). Furthermore, detailed genetic and molecular studies in Pristionchus pacificus indicated that even if the cells forming the vulva are homologous, multiple changes can occur at the genetic and molecular levels. For example, the homeotic genes lin-39 and mab-5 or the even-skipped homolog vab-7 have different functions during vulva formation in P. pacificus and C. elegans (Eizinger and Sommer, 1997; Sommer et al., 1998; Jungblut and Sommer, 1998; Jungblut and Sommer, 2001).
In particular, four important differences have been identified between vulva development in P. pacificus and C. elegans. First, non-vulval cells in the anterior and posterior body region fuse with the surrounding hypodermis in C. elegans, but die of programmed cell death in P. pacificus (Fig. 1A) (Eizinger and Sommer, 1997). Second, vulva induction relies on a continuous interaction between several cells of the somatic gonad and the vulval precursor cells (VPCs) rather than an interaction of the single AC, as in C. elegans (Sigrist and Sommer, 1999). Third, P8.p represents a novel cell type in P. pacificus and is involved in multiple cell-cell interactions during vulva formation, not known in C. elegans or other nematodes (Jungblut and Sommer, 2000). For instance, P8.p inhibits P5.p and P7.p to adopt the 1° cell fate, a process called lateral inhibition. Additional experiments also indicated that the mesoblast M is involved in lateral inhibition and that P8.p and M interact to inhibit the fate of both VPCs (Jungblut and Sommer, 2000). In contrast, no interaction between the P8.p and the M cell has been observed in C. elegans.
We describe the cell lineage of the mesoblast M in P. pacificus and determine which cells of the M lineage interact with the VPCs in P. pacificus. We show that the mesoblast M has an identical cell lineage to that in C. elegans. Lateral inhibition of P(5,7).p requires cells of both major M sublineages, the dorsal and ventral lineage, respectively. In Ppa-mab-5 mutants, the complete M lineage is misspecified. The first two cell divisions occur along a longitudinal axis instead of the dorsoventral and the left/right division axes, resembling a phenotype known for mutations in the C. elegans Twist gene. Furthermore, no proper sex myoblasts are formed in Ppa-mab-5 mutant animals, causing a strong egg-laying defect. In contrast, mab-5 mutants in C. elegans form normal SM cells indicating yet another difference between Ppa-MAB-5 and Cel-MAB-5 function. Finally, the ectopic differentiation of P8.p in Ppa-mab-5 mutants depends on the misspecification of the M lineage and requires at least one inhibitory and one inductive interaction, both of which might be neomorphic.
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MATERIALS AND METHODS |
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Cell ablation experiments
Cell ablation experiments were carried out using standard techniques described for C. elegans (Epstein and Shakes, 1995) and using a Laser Science dye laser of the type described previously (Avery and Horvitz, 1987). Animals were picked into M9 buffer placed on a pad of 5% agar in water containing 10 mM sodium azide as anaesthetic.
Cell lineage characters and cell fate terminology
The different cell fates of the VPCs are distinguished using the terminology 1°, 2°, 3° and 4° for cell fates, and T (transverse), L (longitudinal), N (non-dividing) and O (oblique) for cell division patterns (Sommer and Sternberg, 1995; Sommer and Sternberg, 1996). During normal development, P6.p has the 1° fate and generates six progeny with the cell division pattern TNNT. The two N cells (P6.pap and P6.ppa), which do not divide (in contrast to C. elegans), attach to the AC. P(5,7).p have a 2° fate and generate seven progeny each, with a cell division pattern LLLN (for P5.p). After ablation of other VPCs, an isolated 1° and an isolated 2° cell can be distinguished from one another by several cell lineage characteristics. In the intermediate four-cell stage (after two rounds of cell divisions of a VPC) of a 1° cell, the AC moves between the two central cells P6.pap and P6.ppa. In the final six-cell stage, the cells are located symmetrically around the AC. In the four-cell stage of a 2° cell, the AC does not move between the central Pn.pxx cells. When the invagination is formed, the distribution of the seven progeny is asymmetric and variable with respect to the AC. VPCs that remain epidermal in the absence of vulva induction were designated as 3°. The fate of P8.p was designated as 4° based on the finding that this cell loses its competence to form vulval tissue during early larval development (Jungblut and Sommer, 2000).
Coelomocyte staining
Coelomocytes were stained using rhodamine injections. J2 and J3 stage animals were injected into the pseudocoelomic space with rhodamine dextran (Sigma Cat. No R-8881). 6-8 hours later, the number of coelomocytes was scored using fluorescence miscroscopy.
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RESULTS |
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Negative signaling by P8.p refers to the observation that VPCs can differentiate and form vulva-like tissue after both Z(1,4) and P8.p are ablated together. 18% of the VPCs differentiated in this experiment, whereas after Z(1,4) ablation no vulva differentiation was observed. Thus, P8.p in P. pacificus provides a negative signal that antagonizes inappropriate vulva differentiation (Jungblut and Sommer, 2000).
Lateral inhibition refers to the observation that P5.p and P7.p, but not P6.p, are unable to adopt the 1° cell fate in the presence of P8.p after the ablation of other VPCs (Jungblut and Sommer, 2000). For example, if P(6,7).p are ablated at hatching, P5.p has a 2° fate in the majority of ablated animals (Table 1A). In contrast, if P(6-8).p are ablated, P5.p predominantly adopts the 1° fate (Jungblut and Sommer, 2000). Further experiments had indicated that lateral inhibition was mediated by the M cell lineage. After ablation of P(6,7).p and M, P5.p had a 1° fate in the presence of P8.p indicating for the first time that an interaction between a Pn.p cell and the M cell influences vulval fate specification (Table 1B) (Jungblut and Sommer, 2000). Further evidence for an interaction between Pn.p cells and the M lineage came from the observation that the ectopic differentiation of P8.p in Ppa-mab-5 mutants was dependent on a signal from the M lineage. In unablated Ppa-mab-5(tu74) mutant animals, P8.p differentiates in 80% of the animals. If M was ablated at hatching, differentiation of P8.p was strongly reduced (Jungblut and Sommer, 2000). Taken together, these results suggested the importance of interactions between the M lineage and the Pn.p cells, in particular P8.p.
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Our experiments show that lateral inhibition requires multiple cells of the M lineage, which derive from the two major M sublineages. When we ablated P(6,7).p and M.v or M.d, P5.p had a 1° fate in the majority of cases. Specifically, P5.p had a 1° fate in 69% and 61% of the animals (Table 1C,D). Thus, ablation of either the ventral or the dorsal M sublineage results in the elimination of lateral inhibition. These results indicate that lateral inhibition requires multiple cells and cell types of the M lineage. For example, the interaction involves the sex myoblasts as indicated by the ablation of only the SM cells (Table 1E). However, it is not restricted to the SM cells because the ablation of M.d has a similar effect (Table 1D). Together, these data suggest that the signal acts over a distance and probably in a quantitative manner.
P. pacificus SM migration depends on a gonadal signal
A C. elegans cell-cell interaction that involves multiple cells of various sublineages is the anterior migration of the SM cells. Several guidance mechanisms are involved in this process in C. elegans, viz. a gonad-dependent attraction, a gonad-independent attraction and a gonad-dependent repulsion (Fig. 1B) (Stern and Horvitz, 1991; DeVore et al., 1995; Burdine et al., 1998; Branda and Stern, 2000). If the somatic gonad is ablated in C. elegans, the SM cells are no longer correctly positioned in the center of the gonad. However, the SM cells still migrate anteriorly, indicating that this movement is guided by a gonad-independent attraction. The analysis of egl-17 mutants revealed the existence of yet another gonad-dependent signal affecting SM migration. In egl-17 mutants, the SM cells stay in the posterior body region indicating that under these mutant conditions, the gonad repels the SM cells (Stern and Horvitz, 1991). It is unknown, how the gonad-independent and the gonad-dependent attraction dominate over the gonad-dependent repulsion under wild-type conditions.
We sought to determine if SM migration in P. pacificus also relies on a gonad-dependent guidance mechanism and therefore, ablated Z(1,4) in wild-type animals at hatching. SM cell position is variable after gonad ablation, as in C. elegans (Fig. 3). Also, the SM cells divide and differentiate independently of their final position (Fig. 3). Most of the 24 SM cells studied after gonad ablation stopped migrating and started to differentiate in a region between P6.p and P7.p. Thus, the SM cells remain in a position slightly more posterior than in wild-type animals, a result that is qualitatively and quantitatively similar to observations in C. elegans (Thomas et al., 1990). Therefore, our results demonstrate that in wild-type animals SM migration in P. pacificus is influenced by a gonad-dependent and a gonad-independent guidance mechanism.
Ppa-mab-5 mutants show various M lineage defects that resemble the C. elegans Twist and mab-5 phenotypes
Previous studies indicated that the mesoblast M is misspecified in Ppa-mab-5 mutants (Jungblut and Sommer, 2000). Given the M cell lineage analysis in P. pacificus, we sought to determine which cellular aspects of the M lineage are altered in Ppa-mab-5 mutants. In C. elegans, the requirement of MAB-5 in M lineage patterning has been studied in detail (Kenyon, 1986; Harfe et al., 1998; Corsi et al., 2000; Liu and Fire, 2000). The Cel-mab-5 mutant exhibits, (i) variable defects in the division planes during the first divisions of M, (ii) the absence of the M-derived coelomocytes and (iii) the transformation of some body-wall muscles and the coelomocytes into sex myoblast-like cells (Harfe et al., 1998; Liu and Fire, 2000; Corsi et al., 2000).
We analyzed the role of Ppa-mab-5 in M lineage specification by studying the strong reduction-of-function alleles tu74 and tu31 (Jungblut and Sommer, 1998). We found that strong reduction of mab-5 function in P. pacificus causes strong early and late lineage defects in the M lineage, providing a pattern of similarities and differences with respect to the Cel-mab-5 mutant. First, a strong lineage defect was seen in the first two divisions in the M lineage. In 35 of 36 analyzed Ppa-mab-5 animals, M divided in the anteroposterior direction instead of a dorsoventral division (Table 2A). Also, the two daughters of M divided in the anterior-posterior direction instead of a left-right division in 35 out of 36 animals (Table 2A). As a result, the four descendants of M are in the right ventral quandrant in Ppa-mab-5 mutants (Fig. 4B). This reversal of division axes in the early M divisions is rarely seen in Cel-mab-5(lof) mutants, but is known from mutants in Cel-hlh-8, the C. elegans Twist gene (Corsi et al., 2000).
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To study if coelomocytes and SM cells are formed at all in Ppa-mab-5 mutants we looked specifically for these two cell types at later stages of development. We found that only one out of 24 analyzed Ppa-mab-5 animals had a SM cell in the central body, which formed vulval and uterine muscles later in development (Table 2C). In the other 23 animals, no SM-like cells where present in the central or the more posterior region. Thus, in contrast to Cel-mab-5 animals, which show extra SM-like cells, SM cells are absent in Ppa-mab-5 mutants. This observation also explains why Ppa-mab-5 animals are egg-laying defective, whereas Cel-mab-5 animals are egg-laying positive. However, the egg-laying defect of Ppa-mab-5 mutants is not as penetrant as the SM defect as only 13 of 23 animals that were shown by lineage analysis to lack SM cells were egg-laying defective (Table 2C). Thus, P. pacificus hermaphrodites are, to a certain degree, able to lay eggs in the absence of functional vulval and uterine muscles. This result has been confirmed by ablating the M or SM cells in wild-type animals, which results in approximately 20% of animals that are egg-laying positive (data not shown).
Though the SM cell phenotypes of Ppa-mab-5 and Cel-mab-5 mutants are different, both mutants have a similar coelomocyte phenotype. We counted the number of coelomocytes by rhodamine dextran injection of young mutant and wild-type animals. In 10 out of 11 wild-type animals (91%) six coelomocytes were observed, however only 12% had more than four coelomocytes in Ppa-mab-5 mutant animals (Table 2D; Fig. 4G,H). Taken together, the misspecifation of the M lineage in Ppa-mab-5 animals is much stronger than in Cel-mab-5 mutants.
The M lineage influences P8.p differentiation in at least two distinct interactions in Ppa-mab-5 mutants
Previous cell ablation studies showed that the ectopic differentiation of P8.p in Ppa-mab-5 mutants relies on an induction by the M lineage. After ablation of Z(1,4) in Ppa-mab-5 mutants, P8.p and P7.p were able to differentiate in a gonad-independent manner (Jungblut and Sommer, 1998; Jungblut and Sommer, 2000). However, the differentiation of P8.p decreased from 80% to 22% after the ablation of M at hatching in Ppa-mab-5(tu74) mutant animals (Table 3A,B; Jungblut and Sommer, 2000). Given the M lineage analysis in P. pacificus wild-type and the various M lineage defects in Ppa-mab-5 animals, the question arises of which cells of the M lineage are involved in the ectopic induction of P8.p. To address this question, we ablated the M cell or all of its descendants at various time points in development in Ppa-mab-5(tu74) animals.
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When we ablated the descendants of M later in development, we found that they provide an inductive signal for P8.p differentiation. After ablation of the four progeny of M, 12-13 hours after hatching, P8.p differentiation was observed in approximately 50% of the animals (Table 3D). Specifically, P8.p differentiated in seven out of 15 animals (P<0.0006, 2-test; Table 3C,D). These results suggest that the misspecified M lineage in Ppa-mab-5 mutants provides an inhibitory signal early in development and an inductive signal later in vulva development. Most likely, several of the descendants of M are involved in this induction. However, as the exact cell lineage of M is misspecified and variable in these mutants, we were unable to assign inductive activity to single cells.
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DISCUSSION |
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In this study, we provide the complete M lineage from P. pacificus hermaphrodites. Surprisingly, the lineage is identical to C. elegans hermaphrodites, which also generate 14 body muscles, two coelomocytes and a total of 16 vulval and uterine muscles. Nonetheless, some of these cells play a role during vulval fate specification, a function unknown from C. elegans. The observed similarity of the M lineage between P. pacificus and C. elegans is in contrast to a previous study in Panagrellus redivivus. Sternberg and Horvitz (Sternberg and Horvitz, 1982) showed that females of P. redivivus generate only eight body muscles, two coelomocytes and 12 sex muscles. The reduced number of muscle progeny results from the programmed cell death of six intermediate precursor cells (Sternberg and Horvitz, 1982). It should be noted however, that there are only quantitative differences with respect to the number of cells generated, whereas the same cell types are formed by M in all three studied species.
The mesodermal lineage influences vulval cell fates by complex interactions involving multiple cells
In C. elegans, interactions between different parts of the egg-laying system can occur between single cells, as in the case of vulva induction by the gonadal AC (Kimble, 1981) or between multiple cells as indicated for the role of the somatic gonad in guiding SM migration (Branda and Stern, 2000). Given the new interaction of M with the VPCs, the question arises of whether these interactions rely on single or multiple cells. We found that both major sublineages, the ventral and the dorsal M lineage, are involved in lateral inhibition of P5.p. If P(6,7).p and M.d or M.v were ablated, P5.p had a 1° fate in the majority of cases, whereas P5.p had a 2° fate after the ablation of P(6,7).p alone (Table 1). This finding allows two major conclusions. First, the elimination of one half of the system abolishes the complete interaction. Several mechanisms could account for this observation. For example, lateral inhibition might require a certain amount of a secreted substance. The ablation of M.d or M.v, could reduce the quantity of this substance below a critical threshold, as a result of which lateral inhibition no longer occurs. Second, lateral inhibition most likely acts over a distance as none of the cells of the M.d lineage is in direct contact with P(5,7).p. It remains unknown however, how many cells in each sublineage are involved in lateral inhibition. Also, the exact time point cannot be identified as with the ongoing cell divisions in the M.d and M.v lineage, the number of cells increases over time and the cell division patterns become irregular.
The finding that multiple cells of the M.d and M.v sublineage are involved in lateral inhibition is in agreement with the cellular mechanism of vulva induction in P. pacificus. Several cells of the somatic gonad of different sublineages are required for proper vulva induction to take place (Sigrist and Sommer, 1999).
The gonad guides the migration of the sex myoblasts
SM migration in C. elegans hermaphrodites is controlled by the interaction of at least three guidance mechanisms involving a gonad-dependent and a gonad-independent attraction (Stern and Horvitz, 1991; DeVore et al., 1995; Burdine et al., 1998; Branda and Stern, 2000). We found that the SMs cells in P. pacificus migrate anteriorly in gonad-ablated animals suggesting that wild-type animals contain both a gonad-dependent and a gonad-independent guidance mechanism. These experiments, however, do not indicate if a gonad-dependent repulsion also exists, as in C. elegans. To obtain further insight, SM migration-defective mutants have to be isolated in P. pacificus. In C. elegans, SM migration-defective mutants have been isolated as egg-laying defective mutants. Unfortunately, work described here indicates that a substantial amount of Ppa-mab-5 mutant animals are egg-laying positive although no SM cells are generated. Thus, at least partial egg-laying can occur in P. pacificus in the absence of a complete egg-laying apparatus, which complicates the genetic isolation of SM-defective mutants.
Novel functions of Ppa-MAB-5 in mesodermal patterning
Mesodermal patterning in P. pacificus and C. elegans is specified during embryonic and postembryonic development. In recent years, several genes involved in postembryonic mesodermal patterning have been identified in C. elegans, involving several transcription factors: the homeodomain factor MAB-5 (Kenyon, 1986; Costa et al., 1998), HLH-8, a Twist homolog (Harfe et al., 1998), the C. elegans E/Daughterless homolog (Krause et al., 1997) and CEH-20, the extradenticle homolog (Liu and Fire, 2000).
Genetic and molecular studies of P. pacificus vulva development identified Ppa-mab-5 as an important patterning gene, the absence of which results in ectopic vulva differentiation of P8.p (Jungblut and Sommer, 1998). In this study we show that Ppa-mab-5 mutants have multiple defects in the M lineage resembling both the Cel-mab-5 and the Cel-hlh-8 genes (Table 4). For example, the division axes of M and Mx are strongly altered from wild-type in Ppa-mab-5 mutants. The penetrance of this defect is much stronger in Ppa-mab-5 than in Cel-hlh-8 and Cel-mab-5 mutants (Table 4).
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The interaction between M and P8.p is complex and involves multiple cells
The ectopic differentiation of P8.p in Ppa-mab-5 animals represents another novel aspect of MAB-5 function, which is not present in Cel-mab-5. Previous studies have indicated that the M lineage is involved in the induction of P8.p differentiation in the Ppa-mab-5 mutant (Jungblut and Sommer, 2000). However, more detailed cell ablation studies of the M lineage at different time points during development suggest a more complex interaction between M and P8.p (Table 3). During early larval development, the M lineage provides an inhibitory signal that antagonizes P8.p differentiation, whereas later in development, M and its descendants induce P8.p differentiation in the absence of MAB-5 function (Fig. 5). It remains unknown, which tissue could be the target for the inhibition by the M lineage early in larval development. One candidate could be the gonad. To test this hypothesis, we ablated Z(1-4) and M simultaneously in Ppa-mab-5 mutants, but observed P8.p differentiation to a similar degree as in M ablated animals alone (data not shown). Another hypothesis is, that complex interactions among the VPCs are of importance, and cell ablation studies to address this question have been initiated (M. Zheng and R. J. S., unpublished observation). Finally, it should be noted that we cannot rule out that the observed interaction between P8.p and the M lineage in Ppa-mab-5 mutants is neomorphic and that no similar interactions exist in wild-type animals. If M or P8.p are ablated in wild-type animals, normal vulval patterning is observed as a result of the redundant nature of several overlapping specification mechanisms (Jungblut and Sommer, 2000).
Nematode developmental evolution and the C. elegans anchor cell
The first inductive interaction discovered in nematode postembryonic development was the induction of the vulva by the anchor cell (Kimble, 1981). Given the invariant cell lineage and the small cell number it was a general believe that cell-cell interactions in nematodes are simple, involving only a small number of cells. However, during the last 10 years, evidence from two different research fields argue against this observation. First, the analysis of other postembryonic processes in C. elegans showed the involvement of multiple cells in cell-cell interactions (Newmann et al., 2000). Second, independent evidence comes from the evolutionary analysis of vulva formation. From the study of the evolution of vulva formation in more than 50 species of seven families, it is obvious that the case of the AC is specific for the genus Caenorhabditis (Sommer, 2000). In other nematodes, vulva development might depend on two separate inductions, on a continuous induction or might occur in a gonad-independent way. Thus, the textbook example of vulva induction by a single cell is an exception. Also, phylogenetic projection of vulval character states indicated that vulva induction by the C. elegans AC represents a derived character (Sommer, 2000). In summary, it seems that cell-cell interactions during postembryonic development in nematodes are mostly complex, involving multiple cells, often of different sublineages within one tissue.
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ACKNOWLEDGMENTS |
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REFERENCES |
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Avery, L. and Horvitz, H. R. (1987). A cell that dies during wild-type C. elegans development can function as a neuron in a ced-3 mutant. Cell 51, 1071-1078.[Medline]
Branda, C. S. and Stern, M. J. (2000). Mechanisms controlling sex myoblast migration in Caenorhabditis elegans hermaphrodites. Dev. Biol. 226, 137-151.[Medline]
Burdine, R. D., Branda, C. S. and Stern, M. J. (1998). EGL-17(FGF) expression coordinates the attraction of the migrating sex myoblasts with vulval induction in C.elegans. Development 125, 1083-1093.
Chang, C., Newman, A. P. and Sternberg, P. W. (1999). Reciprocal EGF signaling back to the uterus from the induced C. elegans vulva coordinates morphogenesis of epithelia. Curr. Biol. 9, 237-246.[Medline]
Corsi, A. K., Kostas, S. A., Fire, A. and Krause, M. (2000). Caenorhabditis elegans twist plays an essential role in non-striated muscle development. Development 127, 2041-2051.
Costa, M., Weir, M., Coulson, A., Sulston, J. and Kenyon, C. (1998). Posterior pattern formation in C. elegans involves posterior specific expression of a gene containing a homeobox. Cell 55, 747-756.
DeVore, D. L., Horvitz, H. R. and Stern, M. (1995). An FGF receptor signaling pathway is required for the normal cell migrations of the sex myoblasts in C.elegans hermaphrodites. Cell 83, 611-620.[Medline]
Eizinger, A. and Sommer, R. J. (1997). The homeotic gene lin-39 and the evolution and nematode epidermal cell fates. Science 278, 452-455.
Epstein, H. F. and Shakes, D. C. (1995). Caenorhabditis elegans: Modern Biological Analysis of an Organism. Methods in Cell Biology, Vol. 48 San Diego: Academic Press.
Félix, M. A. and Sternberg, P. W. (1997). Two nested gonadal inductions of the vulva in nematodes. Development 124, 253-259.
Félix, M. A. and Sternberg, P. W. (1998). A gonad-derived survival signal for vulval precursor cells in two nematode species. Curr. Biol. 8, 287-290.[Medline]
Félix, M. A., De Ley, P., Sommer, R. J., Frisse, L., Nadler, S. A., Thomas, K., Vanfleteren, J. and Sternberg, P. W. (2000). Evolution of vulva development in the Cephalobina (Nematoda). Dev. Biol. 221, 68-86.[Medline]
Greenwald, I. (1997). Development of the vulva. In C. elegans II (ed. D. L. Riddle, T. Blumenthal, B. J. Meyer and J. R. Priess), pp. 519-542. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
Harfe, B. D., Gomes, A. V., Kenyon, C., Liu, J., Krause, M. and Fire, A. (1998). Analysis of a Caenorhabditis elegans twist homolog identifies conserved and divergent aspects of mesodermal patterning. Genes Dev. 12, 2623-2635.
Hill, R. J. and Sternberg, P. W. (1992). The gene lin-3 encodes an inductive signal for vulval development in C. elegans. Nature 358, 470-476.[Medline]
Jungblut, B. and Sommer, R. J. (1998). The Pristionchus pacificus mab-5 gene is involved in the regulation of ventral epidermal cell fates. Curr. Biol. 8, 775-778.[Medline]
Jungblut, B. and Sommer, R. J. (2000). Novel cell-cell interactions during vulva development in Pristionchus pacificus. Development 127, 3295-3303.
Jungblut, B. and Sommer, R. J. (2001). The nematode even-skipped homolog vab-7 regulates gonad and vulva position in Pristionchus pacificus. Development 128, 253-261
Kenyon, C. (1986). A gene involved in the development of the posterior body region of C. elegans. Cell 46, 477-487.[Medline]
Kimble, J. (1981). Lineage alterations after ablation of cells of the somatic gonad of Caenorhabditis elegans. Dev. Biol. 87, 286-300.[Medline]
Kimble, J. and Hirsh, D. (1979). Postembryonic cell lineages of the hermaphrodite and male gonads in Caenorhabditis elegans. Dev. Biol. 70, 396-417.[Medline]
Kornfeld, K. (1997). Vulva development in Caenorhabditis elegans. Trends Genet. 13, 55-61.[Medline]
Krause, M., Park, M., Zhang, J.-M., Yuan, J., Harfe, B., Xu, S.-Q., Greeenwald, I., Cole, M., Paterson, B. and Fire, A. (1997). A C. elegans E/Daughterless bHLH protein marks neuronal but not striated muscle development. Development 124, 2179-2189.
Liu, J. and Fire, A. (2000). Overlapping roles of two Hox genes and the exd ortholog ceh-20 in diversification of the C. elegans postembryonic mesoderm. Development 127, 5179-5190.
Moerman, D. G. and Fire A. (1997). Muscle: Structure, Function, and Development. In C. elegans II (ed. D. L. Riddle, T. Blumenthal, B. J. Meyer and J. R. Priess), pp. 417-470. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
Newman, A. P., Inoue, T., Wang, M. and Sternberg, P. W. (2000). The Caenorhabditis elegans heterochronic gene lin-29 coordinates the vulval-uterine-epidermal connections. Curr. Biol. 10, 1479-1488.[Medline]
Sigrist, C. B. and Sommer, R. J. (1999). Vulva formation in Pristionchus pacificus relies on continuous gonadal induction. Dev. Genes Evol. 209, 451-459.[Medline]
Sommer, R. J. (1997a). Evolution and development the nematode vulva as a case study. BioEssays 19, 225-231.[Medline]
Sommer, R. J. (1997b). Evolutionary change of developmental mechanisms in the absence of cell lineage alterations during vulva formation in the Diplogastridae. Development 124, 243-251.
Sommer, R. J. (2000). Evolution of nematode development. Curr. Opin. Genet. Dev. 10, 443-448.[Medline]
Sommer, R. J. and Sternberg, P. W. (1994). Changes of induction and competence during the evolution of vulva development in nematodes. Science 265, 114-118.[Medline]
Sommer, R. J. and Sternberg, P. W. (1995). Evolution of cell lineage and pattern formation in the vulval equivalence group of rhabditid nematodes. Dev. Biol. 167, 61-74.[Medline]
Sommer, R. J. and Sternberg, P. W. (1996). Apoptosis and change of competence limit the size of the vulva equivalence group in Pristionchus pacificus: a genetic analysis. Curr. Biol. 6, 52-59.[Medline]
Sommer, R. J., Carta, L. K., Kim, S. Y. and Sternberg, P. W. (1996). Morphological, genetic and molecular description of Pristionchus pacificus sp. n. (Nematoda: Neodiplogastridae). Fund. Appl. Nemat. 19, 511-521.
Sommer, R. J., Eizinger, A., Lee, K. Z., Jungblut, B., Bubeck, A. and Schlak, I. (1998). The Pristionchus Hox gene Ppa-lin-39 inhibits programmed cell death to specifiy the vulva equivalence group and is not required during vulval induction. Development 125, 3865-3873.
Sulston, J. E. and Horvitz, H. R. (1977). Postembryonic cell lineages of the nematode Caenorhabditis elegans. Dev. Biol. 56, 110-156.[Medline]
Stern, M. J. and Horvitz, H. R. (1991). A normally attractive cell interaction is repulsive in two C. elegans mesodermal cell migration mutants. Development 113, 797-803.[Abstract]
Sternberg, P. W. and Horvitz, H. R. (1982). Postembryonic non-gonadal cell lineage of the nematode Panagrellus redivivus: Description and comparison with those of Caenorhabditis elegans. Dev. Biol. 93, 181-205.[Medline]
Sternberg, P. W. and Horvitz, H. R. (1986). Pattern formation during vulval development in C. elegans. Cell 44, 761-772.[Medline]
Thomas, J. H., Stern, M. J. and Horvitz, H. R. (1990). Cell interactions coordinate the development of the C. elegans egg-laying system. Cell 62, 1041-1052.[Medline]