Department of Molecular Genetics, University of Illinois at Chicago, College of Medicine, Chicago, IL 60607-7170, USA
*Author for correspondence (e-mail: katzen{at}uic.edu)
Accepted 22 October 2001
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SUMMARY |
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Key words: Drosophila, myb, Transcription factor, Mitosis, Centrosomes, Genomic instability
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INTRODUCTION |
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Genomic integrity is maintained by cell cycle checkpoints that monitor DNA replication and detect cellular/DNA damage (Hartwell et al., 1994). Centrosomes organize the poles of the mitotic bipolar spindle and play a key role in genomic stability by ensuring balanced chromosome segregation (Sluder and Hinchcliffe, 1999). When a mitotic cell contains more than two functional centrosomes, a multipolar spindle can be formed that either randomly distributes chromosomes into multiple daughter cells or results in failure to complete cell division (Fukasawa et al., 1996; Sluder and Hinchcliffe, 1999). Either way, the consequence is genomic instability, a hallmark of many aggressive tumors (Pihan et al., 1998; Salisbury et al., 1999). Abnormally high numbers of centrosomes have been detected in many human tumor cells, an anomaly ascribed to their lack of the tumor suppressor proteins p53 (TP53 Human Gene Nomenclature Database), BRCA1 or BRCA2 (Fukasawa et al., 1996; Tutt et al., 1999; Xu et al., 1999).
The vertebrate Myb gene family consists of three closely related members, Myb (c-myb), Mybl1 (A-myb) and Mybl2 (B-myb). Myb genes encode DNA-binding proteins that regulate transcription and have been implicated in regulatory decisions affecting cell proliferation, differentiation and apoptosis (Oh and Reddy, 1999; Weston, 1998). Mutant versions of Myb have been implicated in the genesis of neoplastic disease in chickens, mice and humans (Oh and Reddy, 1999). Drosophila melanogaster possesses a single myb gene (Dm myb; Myb FlyBase), which contains four regions of homology with the vertebrate Myb genes (Bishop et al., 1991; Katzen et al., 1985; Peters et al., 1987). The Dm myb gene product, DMyb (Myb FlyBase), shares several biochemical properties with the vertebrate family of Myb proteins, including binding to a similar consensus sequence and the ability to activate transcription from a reporter construct regulated by vertebrate Myb proteins (Jackson et al., 2001).
We have previously reported the isolation of temperature-sensitive, recessive lethal mutations in Dm myb. Mutant phenotypes revealed a requirement for Dm myb in diverse cellular lineages throughout development (Katzen and Bishop, 1996). Even when mutants are raised at temperatures permissive for viability, adults display abnormalities, including cuticular defects in wings and abdomens. Analysis of the wing phenotype demonstrated that Dm myb is required for both promotion of the G2/M transition and suppression of endoreduplication in pupal wing cells (Katzen et al., 1998).
The abdominal phenotype in Dm myb mutants, which includes missing bristles and patches of undifferentiated and unpigmented cuticle, is also suggestive of a proliferation defect. However, there are notable differences between wing and abdominal phenotypes (see Results), which may reflect the fact that the post-embryonic program for the development of abdominal epidermis is distinct from that of the rest of the adult epidermis (Cohen, 1993; Fristrom and Fristrom, 1993). The epidermis of the thorax (including the wing) and head is formed from imaginal discs, which contain diploid cells that proliferate throughout larval development, completing only their final one or two cell divisions during early pupation. By contrast, the abdominal epidermis is formed from small nests of imaginal cells, called abdominal histoblasts, which do not divide during larval development, but undergo rapid proliferation after puparium formation (Madhavan and Madhavan, 1980).
The differences in Dm myb mutant phenotype between wings and abdomens at the cuticular level, together with the divergences in developmental programs, suggested that an analysis of the cellular basis of the abdominal phenotype might reveal more about Dm myb function. We now report distinct differences between the cellular defects in mutant Dm myb wing and abdominal cells, and we provide evidence that in proliferating abdominal epidermal cells, Dm myb is required to sustain the appropriate rate of proliferation, to suppress formation of supernumerary centrosomes and to maintain genomic integrity.
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MATERIALS AND METHODS |
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Adult abdomen preparation
Matings were performed at 18°C, and female white prepupae were collected and shifted to indicated temperatures. For mutants shifted to non-permissive temperatures, pharate adults were dissected out of pupal cases. Viable and pharate adults were preserved in 100% isopropanol. Abdomens were dissected and immersed in mineral oil for imaging using a Kodak Science Digital MDS 120 camera and a dissecting microscope.
Preparation and fluorescent staining of the abdominal epidermis from larvae and pupae
The enhancer trap insert esgP3, which expresses ß-galactosidase in larval abdominal histoblasts (Hayashi et al., 1993) was used to identify these cells. Wandering third instar larvae were fixed in 4% paraformaldehyde, 0.1% deoxycholic acid, and 0.1% Tween-20 in phosphate-buffered saline (PBS). Samples were then incubated with mouse anti-ß-galactosidase antibodies (Promega, 1:1000), followed by an anti-mouse secondary conjugated to rhodamine (Boehringer Mannheim, 1:10). Finally, samples were stained with DAPI (4'6-diamidine-2-phenylindole) at 0.5 µg/ml, rinsed, and mounted in Vectashield (Vector).
To prepare pupal samples from females of appropriate genotype (see figure legends), control (w), myb1 or myb2 virgin females were mated with myb1, myb2 or Df(1)sd72a/Dp(1:Y)shi+Y#3 males at 18°C. Female progeny were selected as larvae and maintained at 18°C until pupariation. White prepupae (0 hours after puparium formation (APF)) were picked and shifted to indicated temperatures for specified times. Pupae were then treated for abdominal epidermal preparation as previously described (Kopp et al., 1997) before fixation. Fixation conditions varied, depending upon the antisera: for PH3 and Bub1 antibodies, 4% paraformaldehyde for 30 minutes at room temperature; and for CP60, CP190 and ß-tubulin antibodies, 37% formaldehyde for 5 minutes at room temperature. Immunostaining was performed as previously described (Audibert et al., 1996; Theurkauf, 1994) with the following dilutions for primary antibodies: 1:20 for ß-tubulin (Harlan); 1:500 for CP60 and CP190; 1:1000 for Bub1 and PH3 (Upstate Biotech); and 1:10,000 for -tubulin (Sigma). Secondary antibodies conjugated to either FITC or rhodamine were purchased from Boehringer Mannheim and used at recommended dilutions. After immunostaining, samples were treated with DAPI at 0.5 µg/ml for 10 minutes, rinsed and mounted. DAPI staining was used to determine and compare the number of abdominal epidermal cells in control and mutant Dm myb animals (see Results and Table 1). BrdU labeling was performed as described by Shermoen (Shermoen, 2000), with the only modification being that samples were incubated with BrdU for 1 hour. Samples were imaged either by confocal microscopy (Zeiss LSM 550) or by wide-field microscopy (Zeiss Axioplan2) using a Princeton Instruments Micromax cooled CCD camera.
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Quantitation of DNA content
Abdominal epidermal samples were fixed in 4% paraformaldehyde for 30 minutes and then stained with rhodamine-conjugated phalloidin (Molecular Probes, 5 U/ml) for 30 minutes and DAPI (0.5 µg/ml) for 10 minutes. After mounting in Vectashield, cover slips were sealed and samples were immediately imaged by confocal microscopy (Zeiss LSM 550) under identical conditions, set such that the DAPI fluorescence was within a linear range. Stacks of optical z-sections were collected at 2/3 µm intervals. Relative DNA contents of mutant and wild type nuclei were determined using a method similar to those described by others (Euling and Ambros, 1996; Hayashi and Yamaguchi, 1999). Briefly, the public domain NIH Image program 1.61 (http://rsb.info.nih.gov/nih-image/) was used: images were stacked, and the mean fluorescence value of each nucleus for each layer was determined and then multiplied by the area of the nucleus. Total values were obtained for each nucleus by summing the values for each layer. Background levels of signal were measured for all samples. They were low and did not differ significantly between samples.
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RESULTS |
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The abdominal phenotype in Dm myb mutants resembles those reported for certain mutant alleles of escargot (esg), which encodes a zinc-finger transcription factor, and for Drosophila cdc2 (Hayashi et al., 1993; Stern et al., 1993) (Fig. 1H). These defects have been attributed to a failure to suppress endoreduplication in abdominal histoblasts during larval development, thereby producing polyploid abdominal histoblasts that are no longer capable of dividing after puparium formation (Hayashi, 1996; Hayashi et al., 1993). Given the similarity of the phenotypes and our previous finding that when mutant Dm myb wing cells were arrested in G2 of their final cell cycle, they entered into an endoreduplication cycle, it seemed likely that the cellular basis of the myb phenotype would be the same as it is in esg and cdc2 mutants. We used the enhancer trap line esgP3 (Hayashi et al., 1993) to identify abdominal histoblasts in third instar larvae of wild type, mutant Dm myb, and mutant esg animals (Fig. 1I-L). As expected, abdominal histoblast nuclei of esg mutant larvae were much larger than wild type (Fig. 1L). By contrast, no abnormalities were observed in either the number or appearance of the abdominal histoblasts of mutant Dm myb larvae (Fig. 1J,K), indicating that despite similarities in the adult cuticular phenotypes, the cellular basis of these defects in Dm myb mutants is different than in esg and cdc2 mutants.
Abdominal histoblasts that are mutant for Dm myb proliferate more slowly than wild-type cells
As no defects were apparent in the larval abdominal histoblasts of Dm myb mutants, we examined their proliferation during pupal development. Female animals were staged as white pre-pupae and incubated at 24°C (unless otherwise indicated) for specified time periods. Wild-type controls carried the parental white (w) chromosome from which the Dm myb mutants were generated, over a deficiency chromosome (Df(1)sd72a) that deletes the Dm myb gene. The deficiency chromosome was used experimentally to reduce the Dm myb activity of the hypomorphic alleles. No significant differences were observed between w homozygotes and w/Df(1)sd72a females (not shown).
Several types of defects were observed in this analysis. When abdominal epidermal cells were treated with the DNA stain DAPI, it was apparent that abdominal epidermal development was considerably slower in Dm myb mutants than in wild-type controls (Fig. 2). For example, myb2 abdomens were less developed at 42 hours APF than were control abdomens at 30 hours APF (Fig. 2B,E). When compared with controls at 18 and 24 hour APF, there were approximately half the number of histoblast nuclei in myb2 mutants, indicating that mutant cells were about one cell division cycle behind. The delay was more pronounced when Dm myb activity was further reduced by either carrying myb2 over Df(1)sd72a (non-permissive for viability at 24°C; Fig. 2G) or by shifting myb2 homozygotes to 28°C (not shown). At 18 and 24 hours APF, myb2/Df(1)sd72a animals had an average of one-sixth (ranging from one-eighth to a quarter) the number of abdominal histoblasts as controls, suggesting a lag of about two to three cell cycles. Double staining with DAPI and rhodamine-labeled phalloidin (an F-actin specific stain that highlights cell boundaries) showed that at these time points, each cell contained a single nucleus (not shown).
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In previous studies, the myb1 allele displayed a stronger phenotype than myb2 when the flies were raised at the same temperature (Katzen and Bishop, 1996; Katzen et al., 1998). This is not the case, however, in abdominal development. At 24°C, the rate of abdominal histoblast proliferation and spreading for myb1 was somewhat faster than for myb2 (Fig. 2I), and the mitotic defects were similar for both alleles. At 18°C, the rate of abdominal development appeared to be nearly normal in both myb1 and myb2 mutants (not shown). As the myb1 allele is less healthy and more difficult to work with at 24°C than myb2, we concentrated on the latter for these studies, although similar results were obtained with both mutants.
Delayed and abnormal mitoses occur in abdominal epidermal cells of Dm myb mutants
We used an antibody for a mitotic-specific phospho-epitope on histone H3 (PH3) (Hendzel et al., 1997) to identify mitotic abdominal histoblasts. To compare mutant and wild-type cells at the same time points during pupal development, abdominal epidermal samples were prepared from controls and Dm myb mutants at 24 hours APF. To compare mutant and wild type cells at equivalent stages during later development, it was necessary to use different time points, as Dm myb mutants developed more slowly than wild type. Therefore, samples were prepared from wild-type controls at 30 hours APF (roughly equivalent to 42 hours APF in myb2 mutants; Fig. 2B,E), and from myb2 and myb2/Df(1)sd72a at multiple time points, ranging from 40 to 50 hours APF. In addition to proliferating more slowly, mutant myb cells continued to divide well beyond 41 hours APF, the time when wild type cells become postmitotic (Madhavan and Madhavan, 1980). Mitotic cells were detected through to at least 50 hours APF in both myb2 and myb2/Df(1)sd72a cells (Table 1). In spite of their slower proliferation, at 24 hours APF, the mitotic index of myb2 histoblasts was similar to that of wild-type cells (Table 1). Surprisingly, the mitotic index of myb2/Df(1)sd72a cells at 24 hours APF was higher than for myb2 or controls, and it remained higher throughout the experimental time course. The relatively high mitotic indices in myb2/Df(1)sd72a cells, which are proliferating so slowly, is indicative of delayed progress through mitosis.
We also analyzed the percentage of cells in S-phase, as judged by 5-bromo-2-deoxyuridine (BrdU) incorporation, and found no significant difference in either the total percentage of cells in S-phase or in the percentage of cells in late S (at which point BrdU labeling is confined to chromocenters) (Lilly and Spradling, 1996) between wild type and mutant cells at 24 hours APF (not shown). As mutant myb histoblasts proliferate more slowly than wild type, this finding indicates that S-phase is also lengthened in the mutant cells. The percentage of cells in S-phase decreased in all genotypes at later timepoints, albeit more slowly in mutant cells, a result that is in accordance with their extended proliferation. No conclusions could be drawn about the effects of myb mutations on the gap phases as it was not possible in these samples to determine the percentage of cells in G1 or G2.
The distribution of cells between the various stages of mitosis was significantly different between myb mutants and controls (Table 1). Most notably, there was a higher percentage of mutant cells in the early stages of mitosis, before metaphase, and a corresponding decrease in the percentage of cells in metaphase and anaphase. The number of cells in telophase may be an underestimate as rapid dephosphorylation of histone H3 during telophase makes the PH3 antibody useful for identifying cells in early telophase only (Hendzel et al., 1997; Su et al., 1998). Nevertheless, the percentage of mitotic cells in telophase was lower in Dm myb mutant cells (4% of all scored mitoses in either myb2 or myb2/Df(1)sd72a) than in wild type (11% of all scored mitoses).
Along with the intense PH3 staining patterns characteristic of prophase, metaphase and anaphase, another pattern was observed in many mutant myb cells (Fig. 3). In these cells, the PH3 signal was weaker than is usually observed in wild-type cells, and the chromatin was only partially stained. These nuclei appeared to be in interphase by DAPI staining, but the regions corresponding to the PH3 fluorescent signal also stained more intensely with DAPI, suggesting the onset of chromosome condensation. This PH3 staining pattern has been previously described, and apparently represents a pre-prophase stage that corresponds to the initiation of histone H3 phosphorylation at the G2/M transition (Hendzel et al., 1997). In wild-type cells, this stage must progress rapidly, as it is rarely observed (1% or less of PH3 staining cells). In mutant cells, however, the frequency of pre-prophase cells increases as pupation proceeds, reaching as high as 30% of all PH3 staining cells in myb2 and over 40% in myb2/Df(1)sd72a (Table 1). To determine whether the increase in the pre-prophase staining pattern might be characteristic of cells that continue to divide past the normal cessation of proliferation, we stained abdomens prepared from the Minute mutant, RpS31 (also known as M(3)95A1) (Andersson et al., 1994; Lindsley and Zimm, 1992), with the PH3 antibody. Although mitotic cells were observed through 50 hours APF in this mutant, an increase in pre-prophase cells was not evident (not shown). Therefore, we conclude that the progression of mutant Dm myb cells through the early stages of prophase is abnormally slow.
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Although histoblast proliferation was slower in Dm myb mutants throughout pupal development, mitotic defects were not detected in early divisions (up to 24 hours APF), but became increasingly commonplace later (Table 2). No defects were observed in greater than 99% of wild-type mitoses, whereas abnormalities occurred in as many as 22% of myb2 and 48% of myb2/Df(1)sd72a mitotic histoblasts. One defect, in which two centrosomes were present at, or near each pole during anaphase/telophase (Fig. 4B), occurred more frequently in myb2 (and myb1) abdominal histoblasts than in myb2/Df(1)sd72a, indicating it might be the mildest. Mitotic spindles with two centrosomes at each pole were seen much less frequently in metaphase and early anaphase cells. Therefore, we suspect that this defect reflects precocious separation of the centriole pairs associated with each centrosome. Disengagement of mother and daughter centrioles has been reported to occur during late anaphase/early telophase in Drosophila embryos (Gonzalez et al., 1998; Vidwans et al., 1999), but no signs of separation were detected in wild-type histoblasts (Fig. 4A).
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When a probe representing a 359 bp repeat present in X-chromosome heterochromatin (Dernburg, 2000), was used for FISH on samples prepared from females, two signals were detected in each daughter nucleus during mitosis (Fig. 7A). In myb mutants, cells with tripolar spindles that progressed to anaphase were occasionally observed. Fig. 6B shows an example in which each of the three daughter nuclei displayed a different number of signals, ranging from one to six, demonstrating unequal chromosome segregation, and confirming that some mutant polyploid cells continued to divide with dire consequences for genomic integrity. No example of mitoses forming more than three daughter cells was observed, suggesting that cells with four or more poles never complete mitosis. Instead, we suspect the chromosomes decondense and eventually take on the appearance of interphase nuclei, albeit with abnormal morphology (see below).
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To assess relative DNA contents (Fig. 7C), we measured the relative fluorescent intensities of DAPI-stained nuclei from a series of confocal images (see Materials and Methods). DNA content was calculated on a per cell basis, regardless of nuclear morphology or the presence of more than one nucleus. As expected for proliferating cells, the DNA content of control cells varied over a twofold range, with only 1 of 121 nuclei falling slightly outside the one- to twofold range. By contrast, both the average and range of DNA contents were larger for mutant Dm myb cells than for wild type, with more than 20% of myb2 and 25% of myb2/Df(1)sd72a nuclei falling outside the one- to twofold range. These figures are likely to be an underestimate, as several of the larger mutant nuclei could not be measured because they were not entirely contained within the z-stack of optical sections. The mitotic defects observed in the mutant cells (see Fig. 4, Fig. 5, Fig. 7) indicate that some of the cells within the one- to twofold range were likely to be aneuploid. Variability was especially pronounced in myb2/Df(1)sd72a cells, where small nuclei were occasionally seen that contained less than diploid levels of DNA (approximately half, Fig. 7J), while other nuclei had DNA contents that ranged as high as sevenfold more than the normal diploid levels in wild-type cells. Our results confirm that the abnormal mitoses occurring in Dm myb mutant histoblast cells give rise to unequal chromosome segregation and produce aneuploid and polyploid nuclei.
To investigate whether nuclear morphology abnormalities and polyploidy were already apparent by the time the first centrosomal defects were observed, control and mutant samples at 28-30 hours after puparium formation were prepared as described above. Greater than 100 control and 175 mutant cells (myb2 and myb2/Df(1)sd72a combined) were examined. No examples of cells with either two nuclei or a multilobed nucleus were observed at this stage of development. The DNA content of more than 98% of control and mutant cells varied over the same twofold range as was previously observed for control cells at 30 hours APF. These data indicate that the observed centrosome abnormalities are unlikely to arise from failure to complete mitosis or cytokinesis as these defects do not precede the centrosome defects.
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DISCUSSION |
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Defects in esg and cdc2 mutants have been attributed to a failure to suppress endoreduplication in abdominal histoblasts during larval development when they are normally arrested in G2 (Hayashi, 1996; Hayashi et al., 1993). Although the abdominal phenotype in Dm myb mutants resembles those of esg and cdc2 (Hayashi et al., 1993; Stern et al., 1993), mutant Dm myb histoblast nests contain appropriate numbers of cells with no apparent abnormalities. This indicates that although Dm myb function appears to be required to suppress endoreduplication during an aberrant G2 arrest, it is not essential for keeping abdominal histoblasts in the normal extended G2 phase that persists throughout larval development.
In previous analyses of the mutant myb phenotype, the myb1 allele inevitably produced a stronger phenotype than myb2 at equivalent temperatures (Katzen and Bishop, 1996; Katzen et al., 1998). By contrast, the abdominal phenotype is as strong or stronger in myb2 than in myb1 at the same temperatures (see Fig. 1, Fig. 2), indicating that the relationship between the two temperature-sensitive alleles is more complex than previously thought. Although each mutation results in the change of an amino acid perfectly conserved between Myb and its vertebrate counterparts, different regions of the protein are affected: for myb2, the DNA-binding domain; and for myb1, a conserved domain (region IV) near the C terminus for which no specific biochemical activity has yet been ascribed (Katzen et al., 1998). The finding that the myb1 phenotype is stronger than myb2 in wing cells, but weaker in abdominal cells indicates that Myb activity is differentially regulated in these two tissues.
Analysis of the abdominal phenotype in myb mutants expands our understanding of Dm myb function in mitosis
Although several abnormalities are observed in Dm myb mutant abdominal histoblasts, the earliest developmental defects are likely to represent the primary cellular defect. Abdominal histoblast cells in Dm myb mutants appeared normal during larval development, a period during which they are arrested in G2, but their rate of proliferation after puparium formation was considerably slower than for wild-type cells. The sluggish proliferation did not correlate with a commensurate decrease in mitosis. For example, although histoblasts in myb2/Df(1)sd72a animals lagged behind wild-type cells by two to three cell division cycles, the mitotic index in the mutants was considerably higher (5.7% versus 2.7% at 24 hours APF), indicating that at least part of the reduced rate of proliferation in mutants can be attributed to abnormally slow progression through mitosis.
Examination of the mitotic cells revealed an enrichment of cells in the early stages of mitosis (pre-metaphase; see Table 1). Even at 24 hours APF, there is an increased ratio of prophase to metaphase cells, and as development proceeds, an increasing number of cells stall in pre-prophase. Whether the majority of pre-prophase cells ever proceed further into mitosis is unclear. It is also possible that some of the weakly staining PH3 cells were not in pre-prophase, but were instead undergoing chromosome decondensation after having failed to complete mitosis or cytokinesis. We believe these are likely to be in the minority for several reasons: an increased percentage of pre-prophase cells can be seen as early as 24 hours APF, before other mitotic or ploidy abnormalities are evident. Up until 30 hours APF, double staining with rhodamine-labeled phalloidin and DAPI revealed an absolute correspondence of one nucleus per cell. In addition, weakly staining PH3 cells did not have abnormally shaped nuclei, even at later developmental timepoints, as did most cells that failed to complete mitosis; instead, the morphology and PH3 staining of the pre-prophase nuclei closely resembled those shown by Hendzel et al. (Hendzel et al., 1997) and the occasional pre-prophase nuclei that we observed in wild-type cells. One further possibility, which cannot be ruled out at present, is that the regulation of histone H3 phosphorylation is somewhat abnormal in these cells, and therefore, the weak staining may not always reflect pre-prophase. Our scoring of mitotic cells did not distinguish between prometaphase and metaphase, but the Bub1 staining patterns suggest that in the mutants, the metaphase population is likely to be enriched for prometaphase cells. Therefore, we conclude that Dm myb mutant histoblasts appear to suffer delays or partial blocks in chromatin condensation, in the process of kinetochore attachment to the mitotic spindle and in chromosome alignment on the metaphase plate.
The percentage of Dm myb mutant histoblasts with visible mitotic abnormalities increases dramatically as pupal development proceeds, implying that initial defects are compounded in subsequent divisions. These mitotic defects, which include aberrant numbers of centrosomes, grossly abnormal DNA morphology, aneuploidy and polyploidy, are characteristic of situations in which the coordination of centrosome and nuclear cycles has been disturbed. Failure to precisely coordinate centrosome doubling with the nuclear cell cycle produces mitotic cells with less than or greater than two centrosomes, a situation that generally results in the formation of abnormal spindles (Sluder and Hinchcliffe, 1999). Examples of such abnormalities, which inevitably lead to genomic instability, are represented among Dm myb mutant abdominal cells. Mitotic cells with only one centrosome forming a monopolar spindle are occasionally seen. These cells cannot divide and will inevitably produce polyploid cells. More commonly observed are mitotic cells with more than two centrosomes forming multipolar spindles. In such a situation, chromosomes will be randomly distributed into multiple daughter cells which will be aneuploid if cell division is successfully completed. Alternatively, if the mitotic cell fails to complete division and returns to an interphase state, the resulting cell would either be multinucleate or contain a single polyploid nucleus. Our results indicate that all of these possible outcomes occur in mutant Dm myb cells. Therefore, we believe that defects observed during later stages of abdominal epidermal development are the consequence of disturbing the coordination between centrosome reproduction and the nuclear cell cycle.
In a recent study that compared gene expression in actively proliferating fibroblasts isolated from people of different ages or from individuals with the premature aging disease progeria, Mybl2 expression was significantly downregulated in both progeria fibroblasts and in normal fibroblasts isolated from old-aged (90 years) people (Ly et al., 2000). A significant proportion of these aged fibroblasts exhibited abnormalities, including multilobed nuclei and multiple nuclei within a cell. These nuclear defects are reminiscent of those observed in the abdominal epidermal cells of Dm myb mutants, suggesting the possibility that reduced levels of Mybl2 might lead to centrosomal abnormalities, which in turn cause aneuploidy and polyploidy.
Is the disturbance in centrosome regulation a primary defect of the mutation in Dm myb or a secondary consequence of the slow rate of proliferation and/or delays in early mitotic stages, which occur earlier in development? Our analysis indicates that the centrosome defect may be primary, as the first abnormalities in centrosome numbers precede the appearance of multilobed and binucleated cells, allowing us to exclude the possibility that a failure in mitosis or cytokinesis represents the primary defect. Once present, however, the extra centrosomes do lead to defective mitoses and failed cell divisions.
Many links have been established between the regulation of the cell division cycle and centrosome duplication. Artificial prolongation of mitosis leads to premature splitting and separation of centrosomes, while prolongation of S-phase allows multiple rounds of centrosome duplication to occur (Sluder and Hinchcliffe, 1999). The mildest centrosome defect in Dm myb mutants appears to be a premature separation of centriole pairs during late anaphase/early telophase, which could be the result of delayed progression through mitosis. Premature separation might explain why the percentage of centrosome abnormalities is always higher in anaphase/telophase cells than in metaphase cells (see Table 2). This early separation may therefore represent the first step in a breakdown of the coordination between nuclear and centrosome cell cycles.
Another indication that splitting and/or duplication of centrosomes is not occurring in a coordinated fashion is the frequent occurrence of odd numbers of centrosomes within the mutant cells. Four centrosomes in mutant cells could result from failure to complete the previous cell division, followed by re-entry into the cell cycle. A subsequent mitosis could then easily lead to an assortment of an odd number of centrosomes in each daughter cell (e.g. three in one and one in the other), but they should be duplicated during the subsequent cycle, thereby generating an even number by the following mitosis (six and two, respectively). Intriguingly, odd numbers of centrosomes are also a common feature in vertebrate cells that have suffered a mutation in a tumor suppressor gene such as p53, Brca1 or Brca2 (Fukasawa et al., 1996; Tutt et al., 1999; Xu et al., 1999).
Can Myb genes function as both oncogenes and tumor suppressor genes?
The normal function of many proto-oncogenes is to participate in signal transduction pathways that regulate cellular proliferation. When proto-oncogenes suffer mutations that convert them to activated oncogenes, they promote uncontrolled cell growth. Specific aspects of the phenotypic defects observed in the Dm myb mutants, such as sluggish proliferation and stalling or arresting at the G2/M transition, match expectations for loss-of-function mutations in a proto-oncogene. Centrosome amplification and genomic instability, however, are frequently associated with loss-of-function mutations in tumor suppressor genes, not proto-oncogenes (Fukasawa et al., 1996; Tutt et al., 1999; Xu et al., 1999). As genomic instability is associated with oncogenic progression and aggressive tumors, the disturbance in the regulation of centrosome reproduction may be the primary mechanism by which tumorigenesis is promoted when some tumor suppressor genes are mutated (Sluder and Hinchcliffe, 1999; Tutt et al., 1999; Xu et al., 1999). Therefore, the Dm myb gene shares some properties with proto-oncogenes and others with tumor suppressor genes, raising the possibility that mutations which decrease the activity of one of the vertebrate Myb genes could contribute to genomic instability and subsequent oncogenesis or aging.
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ACKNOWLEDGMENTS |
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