1 Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
2 Department of Cell Biology and Physiology and Neuroscience, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
*Author for correspondence (e-mail: joyner{at}saturn.med.nyu.edu)
Accepted September 12, 2001
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SUMMARY |
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Key words: Gli3, Neural patterning, Mouse, Mutants
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INTRODUCTION |
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In vitro explant assays have shown that Shh is able to induce the formation of distinct ventral cell types in a concentration dependent manner; floor plate cells are induced at high concentrations, and motoneurons and three distinct classes of ventral interneurons (V1-V3) at progressively lower concentrations (Ericson et al., 1997). Recent evidence further suggests that this induction is achieved by Shh differentially regulating the expression of cell intrinsic determinants, such as induction of class I homeobox transcription factors and repression of class II transcription factors (Briscoe et al., 2000; Stone and Rosenthal, 2000). However, it is not clear at the molecular level how Shh functions to regulate differentially the expression of these transcription factors.
Shh belongs to the broader Hedgehog (Hh) family of signaling molecules. The molecular mechanisms of Hh signaling pathway have been worked out in greatest detail in Drosophila (Hammerschmidt et al., 1997; Ingham, 1998). At the end of the pathway is the zinc-finger-containing transcription factor Cubitus interruptus (Ci), which appears to be required for all aspects of Hh signaling in Drosophila (Methot and Basler, 2001). Hh signaling controls Ci protein activity at the post-translational level, such that in the absence of Hh signaling, Ci is processed into a truncated repressor form, CiR, which can inhibit some Hh target genes (Aza-Blanc et al., 1997; Robbins et al., 1997). In the presence of Hh signaling, the proteolytic processing of Ci is inhibited, and furthermore, Hh stimulates the maturation of Ci into a labile activator, CiA (Methot and Basler, 1999; Ohlmeyer and Kalderon, 1998). Loss of hh function results in all Ci being converted into CiR, thus resulting in a more severe phenotype in hh mutants than in ci mutants, which do not have CiR (Methot and Basler, 2001).
Three homologs of Ci, Gli1, Gli2 and Gli, have been identified in human and mouse (Hui et al., 1994; Ruppert et al., 1990). The Gli proteins share high homology in their five zinc-finger domains, but limited homology outside of this region (Matise and Joyner, 1999). Gain-of-function studies in embryos have been used in an attempt to compare the properties of the three Gli genes in vivo. Ectopic expression of Gli1, but not Gli2 or Gli3, can induce the expression of a floor plate marker, HNF3ß (Foxa2 Mouse Genome Informatics) in the dorsal CNS of mouse and frog embryos (Hynes et al., 1997; Lee et al., 1997; Marine et al., 1997; Park et al., 2000; Sasaki et al., 1999). When expressed in flies, Gli1 also functions as an activator in Hh signaling (Aza-Blanc et al., 2000; von Mering and Basler, 1999). When Gli2 or Gli3 are injected together with Gli1, they inhibit the ectopic activation function of Gli1 (Ruiz i Altaba, 1998). This may reflect that Gli2 and Gli3 have N-terminal repressor domains (Dai et al., 1999; Sasaki et al., 1999; Yoon et al., 1998). Gli2 has also been shown to function as a weak activator in transgenic flies, and in frog embryos, Gli2 can induce motoneuron development (Aza-Blanc et al., 2000; Ruiz i Altaba, 1998). Gli3, however, has been shown to function as a repressor in Hh signaling in fly and frog embryos (Aza-Blanc et al., 2000; Ruiz i Altaba, 1998; von Mering and Basler, 1999). Of the three Gli genes, only Gli1 expression is dependent on and can be activated by Shh (Grindley et al., 1997; Hynes et al., 1995; Lee et al., 1997; Litingtung and Chiang, 2000; Marigo et al., 1996; Rowitch et al., 1999). Taken together, the mis-expression studies suggest that the three Gli proteins have primarily different functions.
Biochemical studies have shown that Gli3 can be processed like Ci into an N-terminal form, both in fly imaginal disc cells and mouse and chick limbs (Aza-Blanc et al., 2000; Wang et al., 2000). Furthermore, the proteolytic processing of Gli3 was found to be regulated by Hh signaling, such that in the presence of Hh signaling this processing is blocked (Aza-Blanc et al., 2000; Wang et al., 2000). Interestingly, even though Gli2 contains both activator and repressor domains, the processing of frog Gli2 in fly imaginal disc cells was found to be independent of Shh (Aza-Blanc et al., 2000), whereas when Gli2 was over-expressed in COS cells, no processing was detected (Wang et al., 2000). By contrast, Gli1 does not appear to be processed in mouse extracts, or when ectopically expressed in COS7 cells or fly imaginal discs (Aza-Blanc et al., 2000; Dai et al., 1999; Park et al., 2000).
Null mutations in the three mouse Gli genes have been identified or generated. Surprisingly, mice that lack Gli1 function are phenotypically normal (Park et al., 2000) (C. B. B. and A. L. J., unpublished). Mice with mutations in Gli2, however, die at birth and have defects in floor plate and V3 interneuron development, as well as many abnormalities in many other tissues including the skeleton and lungs (Ding et al., 1998; Matise et al., 1998; Mo et al., 1997; Motoyama et al., 1998; Park et al., 2000). The lack of floor plate in Gli2 mutants indicates Gli2 acts downstream of Shh to induce at least some ventral genes. By contrast, mice with a mutation in Gli3 (extra toes, Gli3Xtj) have dominant dorsal brain defects and polydactyly, but have a relatively normal spinal cord (Ding et al., 1998; Theil et al., 1999). As the Gli3 mutant defects are similar to Shh gain-of-function defects, Gli3 may functions to repress Shh targets. Indeed, in Shh;Gli3 double mutants, motoneurons develop in the spinal cord, indicating that a crucial function of Shh is to inhibit the repressor activity of Gli3, to allow motoneuron development (Litingtung and Chiang, 2000).
Interestingly, Gli1;Gli2 double homozygous mutant mice have only slightly more severe CNS and lung phenotypes than either mutant alone, suggesting that Gli1 and Gli2 have few overlapping functions (Park et al., 2000). Gli2;Gli3 mutant analysis revealed overlapping functions of the two genes in skeletal and lung development, probably reflecting similar repressor functions of the two proteins (Hardcastle et al., 1998; Mo et al., 1997; Motoyama et al., 1998). By contrast, Gli1;Gli3 double mutants do not appear to have an enhanced Gli3 phenotype (Park et al., 2000). Because of redundancy in some aspects of gene function and differences in the gene expression patterns of the three Gli genes, the mutant analysis in mice does not provide a direct in vivo comparison of the different Gli functions.
Gene mis-expression studies can provide a quick assay to analyze the potential function of a protein, but the results do not necessarily determine what the gene normally does during development. For example, ectopic expression of Gli1 has been shown to be able to induce floor plate development, and yet Gli1 is not required in vivo for floor plate development. Conversely, ectopic Gli2 expression does not induce floor plate formation, but Gli2 is required for floor plate development in the mouse CNS. Given the many essential roles the Shh pathway plays in mammalian development and disease, it is key to test directly in vivo whether Gli1 and Gli2 have different functions in transducing Shh signaling. We addressed this question directly by replacing the Gli2-coding sequences with those of Gli1 using a gene targeting knock-in approach (Hanks et al., 1995) that ensures that Gli1 is expressed in precisely the same way as Gli2 in the right places and at the right times during embryonic development and postnatally. Given the different biochemical properties and genetic functions of Gli1 and Gli2, a prediction was that replacement of Gli2 with Gli1 would result in activation of Shh targets in Gli2-expressing cells, resulting in the formation of excess floor plate in the CNS. Strikingly, our studies show that a low level of Gli1 can replace all Gli2 functions and rescue the embryonic lethality of Gli2 mutations. When both copies of Gli2 are replaced with Gli1, however, lethality occurs due to new gain-of-function defects, despite CNS patterning being normal. We show that the defects are at least in part due to Gli1 interfering with Gli3 function. Finally, as Gli1 can act as a constitutive activator, we tested whether expression of Gli1 in place of Gli2 can rescue the Shh mutant phenotypes and found Gli1 in this context was insufficient to rescue the ventral spinal cord.
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MATERIALS AND METHODS |
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Generation of knock-in mice
Genomic DNA containing the first three exons of Gli2 was isolated from a 129SvEv genomic phage library (Stratagene). To make the targeting vectors, a 3.2 kb BamHI fragment (from a 5' BamHI to 74 bp before the AUG) was used as a 5' arm and a 6 kb ClaI fragment (from 39 bp after the ATG to a 3' ClaI site) was used as a 3' arm (Fig. 1A). 1ki, 2ki and lzki constructs (Fig. 1B) were then inserted into the targeting vector. The neo cassette is in the opposite orientation compared with the endogenous Gli2 gene. W4 ES cells (Auerbach et al., 2000) were grown, electroporated and subjected to double selections as described (Matise et al., 2000). Targeted clones were identified by BamHI restriction enzyme digestion and Southern blot analysis with a 3' external probe, giving a wild-type band of >13 kb and a mutant band of about 11 kb (Fig. 1C). The same blot was then reprobed with a 5' internal probe, giving a >13 kb wild-type band and 4.7 kb (1ki), 10.3 kb (2ki) and 3.2 kb (lzki) target locus bands. Three targeted cell lines for each vector were then injected into C57 BL/6J blastocysts (Papaioannou and Johnson, 2000). Chimeras were bred with Black Swiss Webster outbred mice and 129S6/SvEv inbred mice (Taconic) to establish F1 heterozygotes. Two independent germline transmitting mouse lines were established for each knock-in construct. PCR analysis was used to routinely genotype knock-in animals or embryos (see Fig. 1 for primer locations). The primers used were: P1, ATGGAGACTTCTGCCCCAGCCCCTGCACTG; P2. ATAAACCC-AGCGTGCCTCCCAGATGACAGG; P3, AGACTGCCTTGGG-AAAAGCG; P4, GTCTTGCCTGAATAGACTGTGGGTGAATCC.
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Immunohistochemistry and RNA in situ analysis
Embryos were fixed in 4% paraformaldehyde for 20 minutes at 4°C before embedding. Frozen sections were cut at 12 µm as described previously (Matise et al., 1998). Monoclonal antibodies (obtained from the Developmental Studies Hybridoma Bank, University of Iowa) for the following proteins were used: Shh, HNF3ß, Nkx2.2, Isl1/2, Pax6, Pax7 (Ericson et al., 1996). Cy3-conjugated anti-mouse secondary antibody was obtained from Jackson ImmnunoResearch and used at 1:500. Images were captured via a Hamamatsu cooled CCD camera and colors were assigned.
Whole-mount RNA in situ hybridization and X-gal staining were performed as previously described (Matise et al., 1998). The in situ probes used were for Gli1 and Gli2 (Hui et al., 1994).
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RESULTS |
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Following electroporation of each vector into W4 ES cells (Auerbach et al., 2000), Southern blot analysis using 5' and 3' probes identified correctly targeted clones (Fig. 1C and see Materials and Methods). At least two independent targeted alleles were transmitted through the germline for each allele following ES cell chimera formation (Papaioannou and Johnson, 2000). Since all the initial mice that were made had a neo cassette, they were designated with an n (for example Gli2n1ki, for Gli1 knock-in into Gli2 with neo). The neo gene in the targeted alleles was then removed by crossing the mice to NLS-CRE-expressing transgenic mice. The resulting mice lacking the neo cassette were designated without an n (for example, Gli21ki).
Homozygous Gli2 knock-in mice (Gli22ki) are normal and lacZ knock-in mutants (Gli2lzki) have defects similar to Gli2zfd mutants
It was expected that insertion of an exogenous cDNA into the second exon of the Gli2 genomic locus would result in the cDNA being expressed precisely in the same manner as the endogenous Gli2 gene. Furthermore, it was expected that the strong polyadenylation signal downstream of the inserted cDNA would terminate transcription after the inserted Gli2 3' UTR. Insertion of a lacZ cDNA into the Gli2 genomic locus should therefore generate a null Gli2 allele, whereas inserting a Gli2 cDNA should result in a normal allele.
Gli2zfd mutants, which lack the exons encoding the zinc-finger DNA-binding domains 3-5, have no floor plate, small lungs that lack the accessory lobe, a lack of notochord regression and skeletal abnormalities (Ding et al., 1998; Matise et al., 1998; Mo et al., 1997; Motoyama et al., 1998; Park et al., 2000). To address whether the new Gli2lzki allele produced the same phenotypes as the Gli2zfd allele, homozygous Gli2lzki embryos were examined and compared with Gli2zfd mutants. In wild-type embryos, Shh and HNF3ß are both expressed in the notochord and floor plate. In the spinal cord of Gli2lzki/lzki E10.5 embryos, as in Gli2zfd/zfd embryos, no cells were present that expressed Shh and HNF3ß (Fig. 2G,H) (Matise et al., 1998). In addition, the number of Nkx2.2 expressing V3 interneurons that are normally adjacent to the floor plate was greatly reduced in both Gli2 mutants (Fig. 2I) (Matise et al., 1998). With the absence of floor plate cells and V3 interneurons, the Isl1/2-expressing motoneurons occupied the ventral midline of the two Gli2 mutant spinal cords (Fig. 2J) (Matise et al., 1998). The expression of dorsal markers of the spinal cord, such as Pax6 and Pax7, however, were largely unaffected in both Gli2 mutants (Fig. 2K,L) (Matise et al., 1998). Furthermore, the lungs of E12.5 Gli2lzki/lzki embryos, as in Gli2zfd/zfd embryos, were reduced in size and the accessory lobe was missing. In addition, the notochord was situated close to the spinal cord in both Gli2 mutants (data not shown).
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Expression of lacZ from the Gli2 allele in Gli2nlzki or Gli2lzki embryos exactly replicated the normal Gli2 expression pattern (data not shown and see Fig. 4, Fig. 5). To further test whether an inserted cDNA was expressed like endogenous Gli2 using our targeting strategy, homozygous Gli22ki mice expression a Gli2 cDNA from the Gli2 allele were generated to determine whether they were normal. Three lines of germline transmitting mice were established and maintained on both inbred and outbred backgrounds. Intercrosses of heterozygous mice produced homozygous knock-in mice at a normal Mendelian frequency, and these animals appeared normal in all respects (Table 1). Taken together, these studies demonstrated a successful knock-in gene targeting strategy with Gli2 function being abolished by insertion of a cDNA into exon 2 and expression of the cDNA precisely in a Gli2-like manner.
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To determine whether one copy of Gli1 can rescue other defects caused by loss of Gli2 function, we examined the lungs and notochord of Gli21ki/ and Gli2n1ki/ embryos at E12.5. Indeed, the accessory lobe, which is absent in Gli2 mutants, was restored in Gli2 knock-in embryos, and the notochord regressed just as in wild-type embryos (data not shown). Furthermore, Gli2n1ki/ mice were born at a Mendelian frequency and appeared to be phenotypically normal throughout life (Table 1). Gli21ki/ mice also appear phenotypically normal at weaning and were present at Mendelian ratio, but later develop a mild hair defect. As Gli1 was only weakly expressed from the Gli2 allele containing neo, these results show that a low level of Gli1 is sufficient to compensate for all Gli2 function during development and postnatally. Furthermore, as Gli1 is reported not to have repressor function (Sasaki et al., 1999), these results suggest that only the activator form of Gli2 is required during embryonic development.
As an additional test of whether any repressor form of Gli2 is required in Shh signaling, we examined whether removing Gli2 rescues any of the Shh mutant defects. However, unlike Gli3/;Shh/ mutants, Gli2/;Shh/ E10.5 embryos (n=4) appeared similar to Shh mutant embryos, with cyclopia (Fig. 5C,G) and loss of ventral cell types including floor plate cells, interneurons and motoneurons in the spinal cord (Fig. 6C,G,K). Collectively, these results demonstrate that unlike Gli3, any Gli2 repressor form that is produced does not play a significant role in repressing Shh targets, at least in the presence of two normal Gli3 alleles.
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Pax7, which is expressed by all dorsal progenitor cells in wild-type embryos, was still expressed only in the dorsal half of Gli21ki/1ki embryos (Fig. 7L), suggesting that the generation of dorsal progenitor cells was not perturbed. The differentiation of the floor plate, ventral interneurons and motoneurons was then examined. In Gli21ki/1ki embryos, expression of Gli1 throughout the lateral and dorsal spinal cord did not result in excess floor plate differentiation, based on Shh and HNF3ß expression (Fig. 7G,H). We further examined the Nkx2.2-expressing V3 interneurons and Isl1/2-expressing motoneurons, both of which lie dorsal to the floor plate cells and can be induced by low levels of Shh. Indeed, both V3 interneurons and motoneurons appeared to be generated correctly in Gli21ki/1ki embryos (Fig. 7I,J). Taken together, these studies demonstrate that the expression of a higher level of Gli1 throughout the spinal cord (except in the floor plate after E8.5, see Fig. 5K) at normal physiological levels for Gli2 does not disturb the generation and patterning of lateral and dorsal neurons in any obvious manner. This suggests that the Shh signaling pathway was not ectopically activated and raised the possibility that Shh signaling might modulate the ability of Gli1 to function in the early ventral spinal cord.
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When one copy of Gli1 was expressed from the Gli2 allele in E10.5 or E11.5 Shh mutant embryos, the head of the embryos was found to be only slightly larger than in Shh/ embryos (10/10). Moreover, in only 50% of the embryos (5/10) development of the diencephalic vesicles was only slightly rescued, based on the finding that grossly the diencephalic vesicles were partially separated into two vesicles (Fig. 5E-H). The generation of a floor plate and ventral neurons in the spinal cord was then examined in E10.5 Gli21ki/+;Shh/ embryos. Strikingly, no floor plate, V3 interneurons or motoneurons could be detected in Gli21ki/+;Shh/ embryos (Fig. 6). Similar results were obtained when a Gli21ki/1ki;Shh/ embryo was examined. These results demonstrated that when Gli1 is expressed from the Gli2 allele, a normal Shh gene is required for Gli1 to activate Shh target genes in the spinal cord.
Homozygous Gli21ki mice die due to gain-of-function effects
Although Gli21ki/ animals are found at the expected Mendelian frequency and live to old age and breed, they were found to develop a skin defect after 3 months of age (data not shown, but see Fig. 8A). As Gli2n1ki/ animals that also lack Gli2 function but express a lower level of Gli1 in place of Gli2 do not develop a hair defect, this suggested the phenotype is due to a gain-of-function effect of Gli1 mis-expression. Consistent with this, Gli21ki/+ animals also were found to develop a similar phenotype to Gli21ki/ animals, which included a gradual loss of hair starting on the dorsal neck and proceeding caudally down the back after the animals reached about three weeks of age (Fig. 8A). The hair loss got worse with time, and at about 5 months, some mice had no hair around the neck (data not shown). By contrast, heterozygous Gli1 knock-in mice with a neo cassette present in the knock-in allele (Gli2n1ki/+) did not develop a hair defect (Fig. 8B).
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Gli1 antagonizes the normal repressor activity of Gli3
The gain-of-function defects seen in Gli21ki/+ mice could be due to activation of Shh target genes in inappropriate cells, and/or due to Gli1 protein interfering with the function of another protein, in particular Gli3. As Gli3 is normally expressed at a distance to Shh-expressing cells and the processing of Gli3 into an N-terminal truncated form in mouse embryos is inhibited by Shh (Wang et al., 2000), Gli3 is likely normally primarily present in a repressor form. As Gli1 is not cleaved and does not have repressor function, co-expression of Gli1 and Gli3 in the same cells in Gli21ki/+ mice could lead to mutual antagonism.
To test whether any part of the Gli21ki/+ gain-of-function phenotype is due to Gli1 interfering with Gli3 function, we compared the phenotype of Gli3+/ mice with that of Gli21ki/+;Gli3+/ mice. The most obvious phenotype in Gli3+/ mice is mild postaxial polysyndactly of the forelimbs and preaxial polysyndactly of the hindlimb (Hui and Joyner, 1993). Strikingly, Gli21ki+/;Gli3+/ double heterozygous mice showed a severe hair loss that occurred throughout the body by six weeks of age (Fig. 8C), and the Gli3+/ polydactyly was enhanced (Fig. 8D). In addition, female double heterozygous mice died by six weeks of age (n=3), and all the male mice were sterile (n=5). As the enhanced phenotypes seen in Gli21ki/+;Gli3+/ double heterozygotes are reminiscent of the gain-of-function phenotypes seen in homozygous Gli2n1ki/n1ki mice, these results provide genetic evidence that Gli1 can antagonize the Gli3 function.
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DISCUSSION |
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Gli1 can replace all necessary Gli2 functions
One of the key observations of this study is that Gli1 can functionally substitute for Gli2 in all processes. Unlike mis-expression studies, where it is difficult to control the timing, level and location of gene expression, we subjected Gli1 to the same precise controls that regulate the expression of the endogenous Gli2 gene and asked whether Gli1 and Gli2 have similar in vivo functions. To our surprise, we found that Gli1 is able to rescue all the Gli2 mutant phenotypes, including defects in floor plate, lung and notochord development. Furthermore, Gli1 is able to rescue the embryonic lethality of Gli2 mutants when expressed at low levels. These results are consistent with previous studies showing that Gli1 and Gli2 recognize a common set of target genes, including HNF3ß, Ptc and Gli1 itself. In cell culture, both Gli1 and Gli2 can activate transcription of reporter genes containing Gli-binding sites, and in transgenic mice, ectopic expression of Gli1 or a form of Gli2 with a deletion in the N terminus activates expression of the gene for HNF3ß in the dorsal midbrain (Sasaki et al., 1999). In transgenic flies, it has also recently been shown that ectopic expression of both frog Gli1 and Gli2 can activate dpp and ptc expression (Aza-Blanc et al., 2000; von Mering and Basler, 1999). Interestingly, in each of these experiments, Gli1 and Gli2 activated the targets with different efficiencies.
Given that Gli1 can replace Gli2 in mice, it raises the question as to why endogenous Gli1 does not compensate for a loss of Gli2 in mutant mice. One likely reason is that transcription of Gli1 is upregulated by Shh signaling (Grindley et al., 1997; Hynes et al., 1997; Lee et al., 1997; Marigo et al., 1996). If all Gli1 transcription is actually dependent on Shh signaling, then in Gli2 mutants, Gli1 transcription would be decreased and unable to compensate for the lack of Gli2. Indeed, in Gli2 mutant embryos, expression of Gli1 is downregulated (Ding et al., 1998).
When Gli1 is expressed from the Gli2 locus it does not induce Shh targets in the spinal cord in the absence of Shh
A surprising result of our studies was that Gli1, when expressed in the dorsal spinal cord from the Gli2 locus, does not alter DV patterning or induce Shh targets in ectopic sites. Furthermore, when Gli1 was expressed from one Gli2 allele in Shh mutant embryos, a floor plate was not induced in the spinal cord. On the surface, the results of our studies appear different from previous transgenic studies in which a human GLI1 cDNA or the same mouse Gli1 cDNA as used in the present studies was found to induce Shh target genes when expressed in the dorsal midbrain, a region that lacks Shh expression (Hynes et al., 1997; Park et al., 2000; Sasaki et al., 1999). A likely explanation for the difference in results is that in the previous transgenic studies Gli1 was expressed at higher levels. Our finding that ectopic expression of Gli1 from the Gli2 allele does cause dominant defects indicates that in some tissues the level of Gli1 may be sufficient to activate target genes.
A possible reason why Shh is required for Gli1 to induce a floor plate in our present study is that one function of Shh is to inhibit the processing of Gli3 into a repressor form in the ventral spinal cord (Litingtung and Chiang, 2000). This function may be required to allow Gli1 to activate appropriate targets when Gli1 is expressed at low levels. An alternative, or additional reason why Shh could be required to induce a floor plate in Gli21ki/ animals is that the Gli1 protein activator function is enhanced by Shh signaling and this enhancement is required when Gli1 is present at low levels. A recent study in transgenic flies supports this conclusion (Aza-Blanc et al., 2000). In anterior compartment cells that are far away from the AP border and do not receive Hh signaling, ectopic expression of frog Gli1 was able to activate dpp and ptc transcription. However, in cells in the posterior compartment of the imaginal disc that receive Hh signaling, the ectopic frog Gli1 activity was enhanced.
A Gli2 repressor form is not required during mouse development
Genetic analysis showed that Gli2/;Gli3+/ embryos have a more severe polydactyly phenotype than Gli3+/ mutants alone (Mo et al., 1997), suggesting that Gli2 and Gli3 have overlapping repressor functions. Indeed, transfection studies have shown that the N-terminal domain of Gli2 contains a repressor domain (Sasaki et al., 1999). In addition, when co-injected with Gli1, Gli2 inhibits the activation function of Gli1 (Ruiz i Altaba, 1998). Recent evidence in transgenic flies suggests that, unlike Ci in fly, some Gli2 protein is constitutively processed into a N-terminal repressor even in the presence of Hh (Aza-Blanc et al., 2000). By extrapolation, this would mean that in mouse embryos, in addition to full-length Gli2 being present, Gli2R should always be present. Given this, an important question was whether Gli2R plays a major role in development.
We addressed this question using two approaches. First, we asked whether removing Gli2R function alleviates the Shh mutant phenotypes, as does removal of Gli3 (Litingtung and Chiang, 2000). If Gli2R plays a role in repressing inappropriate targets in the absence of Shh, then removing Gli2R should partially rescue the Shh mutant phenotypes. We found, however, that the phenotype of Gli2/;Shh/ double mutant embryos was grossly indistinguishable from the Shh/ phenotype. Thus, in spite of the fact that Gl2;Gli3 double mutant analysis indicates that Gli2 does have a repressor function, our studies show that Gli2R does not in fact have a critical function, at least when Gli3 is expressed normally. This finding may indicate that in mice, processing of Gli2 to a repressor form is indeed not regulated by Shh, whereas Gli3 is. We also took a more direct approach to the question by replacing Gli2 with the simple activator Gli1, and analyzing the phenotype of embryos and mice on a Gli2 mutant background. Such embryos would not have the repressor function of Gli2 but would still have an activator function. We showed that Gli2n1ki/ and Gli21ki/ mice are fully viable and develop a normal floor plate, notochord and lungs. Taken together, these two studies show that the repressor form of Gli2 is not required in mice. In this respect, it is interesting to note that CiR is also dispensable during fly embryogenesis (Methot and Basler, 1999).
Gli1 has a function not shared with Gli2
Our finding that Gli1 causes new defects in a dose-dependent manner when expressed from the Gli2 allele, shows that Gli1 possesses an activity different from Gli2. Our genetic studies demonstrate that the hair phenotype and other defects seen in adult Gli2 knock-in mice are due to a gain-of-function effect of Gli1. First, Gli21ki/+ animals that retain normal Gli2 function develop the same skin and hair defects as Gli21ki/ animals. Second, Gli2 homozygous mutant animals that express a low level of Gli1 from the neo containing allele have no hair defects. Thus, Gli1 can compensate for any function of Gli2 in the skin and hair, only when Gli1 is expressed at higher levels are defects seemed.
Antagonism between Gli1 and Gli3
One direct mechanism by which Shh signaling inhibits Gli3 repressor activity is by inhibiting the proteolytic processing of Gli3 into Gli3R (Aza-Blanc et al., 2000; Wang et al., 2000). Another possible mechanism for antagonizing Gli3R would be for Shh to activate Gli1 transcription, thus allowing Gli1 protein to compete with Gli3. Our data provide genetic evidence that the latter possibility occurs in vivo. Mice expressing one copy of Gli1 from the Gli2 allele (Gli21ki/+) have only a mild hair loss that becomes obvious around three months of age. However, if one copy of Gli3 is removed from these mice (Gli21ki/+;Gli3+/), then the Gli21ki/+ hair phenotype becomes much more extreme. In addition, the Gli3+/ polydactyly becomes worse. As Gli2+/;Gli3+/ mice do not have an obvious hair phenotype or enhanced polydactyly, a plausible explanation is that Gli1 can antagonize Gli3 function when both proteins are expressed in the same cells in vivo. These data further demonstrate that Gli1 is not equivalent to Gli2, as Gli2 and Gli3 are expressed in the same cells and are not normally antagonistic.
Surprisingly, it appears that Gli1 can antagonize Gli3 function in some cell types, such as the skin, but not in others, such as cells in the dorsal spinal cord. It is possible that different tissues have different critical thresholds for Gli3R and/or that Gli1 is ectopically expressed at different levels in the various tissues. Alternatively, Gli1 and Gli3 may not recognize all the same targets and the differences are specific to certain cell types.
A question arises as to whether such a mechanism functions in vivo during normal development. By E10.5 in mouse embryos, expression of Gli1 and Gli3 is largely non-overlapping, possibly because Shh signaling leads to repression of Gli3 transcription, as well as activation of Gli1. Furthermore, if Gli1 normally antagonizes the repressor activity of Gli3, then it would be expected that Gli1/;Gli3+/ double mutants would have a partially rescued Gli3 phenotype. At least at a gross level, however, this is not the case (Park et al., 2000), suggesting that in normal development, transcriptional control mechanisms prevent Gli1 and Gli3 from being expressed in the same cells. Nevertheless it is possible that in some disease states, such as in individuals with Gorlins that have heterozygous mutations in PTC, or in tumors that arise from mis-regulation of Shh signaling, that Gli1 and Gli3 are co-expressed and a mutual antagonism contributes to the phenotype.
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ACKNOWLEDGMENTS |
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REFERENCES |
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