1 Department of Anatomy and Research Institute for Natural Science of Dongguk University, College of Medicine, , 2 Department of Anatomy, College of Oriental Medicine, Dongguk University, Kyongju 780-714, Korea, , 3 Division of Biology, California Institute of Technology, Pasadena, CA91125, USA and , 5 Department of Microbiology, College of Natural Sciences, Kyungpook National University, Taegu 702-701, Korea
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Abstract |
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Introduction |
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In nerve cells, local protein synthesis in dendrites may play a role in the regulation of mosaic dendritic protein pools and consequently in the growth and plasticity of synapses (Steward and Banker, 1992). The first morphological indications for local protein synthesis derive from the visualization of synapse- associated polyribosomes in dendrites (Bodian, 1965
; Steward and Banker, 1992
), in particular, during periods of develop- mental and reactive synaptogenesis (Steward and Falk, 1986
). In addition to ribosomes, neuronal dendrites are equipped with a spectrum of translational machinery components such as mRNA (Steward, 1997
; Steward et al., 1998
), tRNA, initiation and elongation factors, and elements of the cotranslational signal recognition mechanism (Tiedge and Brosius, 1996
; Scheetz et al., 1997
; Gardiol et al., 1999
). Furthermore, biochemical studies have shown glycosylation within dendrites (Torre and Steward, 1996
). Therefore, it seems that essential elements required for protein synthesis and post-translational modifica- tions are present at the base of spines.
Less is known on the machinery involved in the protein life cycle within dendritic spines. However, it is believed that rough endoplasmic reticulum (RER) and Golgi apparatus extend into proximal portions of the spine neck (Spacek and Harris, 1997; Gardiol et al., 1999
). Subsynaptic cisternae, which are present close to the postsynaptic differentiations, are shown to be associated with several components involved in synthesis and secretion of proteins such as eIF-2 (initiation factor of the translation), ribosomes, BiP (an ER chaperone), rab1 (ER to Golgi traffic), CTR433 (medial Golgi) and TGN38 (trans-Golgi) (Gardiol et al., 1999
), indicating that many components necessary for the life cycle of certain proteins are present even in dendritic spines. However, little is known about the distri- bution of molecular chaperones in synaptic sites.
In an effort to characterize the molecular composition of the postsynaptic density (PSD), a postsynaptic submembranous protein complex (Siekevitz, 1985; Kennedy, 1997
, 1998
), we determined internal amino acid sequences of a 72 kDa protein in the PSD fraction (named as PSD-72) by protein sequencing. The PSD-72 was identified as a member of the HSP70 family. Here, we report evidence for the presence of both Hsc70 and Hsp70 in the rat cerebral cortex and hippocampus, and discuss possible roles for the synapse-associated HSP70, including a potential candidate system for a synaptic tag.
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Materials and Methods |
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The One-Triton PSD fraction (Cho et al., 1992) was prepared from adult rat (Sprague-Dawley) forebrains by washing synaptosome-enriched fraction with 0.5% Triton X-100 as described previously (Carlin et al., 1980
; Cho et al., 1992
). The One-Triton PSD fraction was resuspended and incubated for 15 min in ice-cold detergents at the indicated concentrations. The pellet and supernatant were separated by centrifugation at 201 800 g for 1 h, and the pellets were resuspended in 40 mM TrisHCl (pH 8.0).
Purification and Sequencing of Internal Peptides of n-Octyl Glucoside-soluble and -insoluble PSD-72
The One-Triton PSD fractions were treated with 1% n-octyl glucoside (OG) (4°C, 15 min), and soluble and insoluble proteins were separated by centrifugation at 200 000 g for 30 min at 4°C. About 7 mg (~10 nmol) of the soluble fraction was concentrated in a Speed-Vac concentrator and fractionated on seven preparative 6% SDSpolyacrylamide gels. The 72 kDa proteins were electroeluted as described previously (Moon et al., 1994) and ~500 µg of the protein was electrophoresed in a 6% SDS gel (50 µg/1.2 mm thick and 10 mm wide well). The protein band was visualized by Coomassie R-250 staining, cut into small pieces (~3 x 4 mm) and fragmented by cyanogen bromide (CNBr, 200 mg/ml in 70% formic acid) as described previously (Sokolov et al., 1989
). The CNBr-cleaved peptides were separated on a 12.517.5% gradient TricineSDS gel (Schagger and von Jagow, 1987
) and transferred to a polyvinylidene difluoride membrane (Millipore, Bedford, MA). The peptide bands were visualized by staining with Coomassie R-250, cut and sequenced. The 72 kDa protein, which remains insoluble in 1% OG, was isolated similarly as above by SDSpolyacrylamide gel electrophoresis (PAGE) and electroelution. About 140 µg (~2 nmol) of the protein was electro- phoresed in a 6% SDS gel, transferred to nitrocellulose membrane (NC) and digested with trypsin as described previously (Cho et al., 1992
). Tryptic peptides were purified on a C18 reverse phase high pressure liquid chromatography column (RP-HPLC) (Cho et al., 1992
). Amino acid sequences were determined by Edman degradation in the Caltech Protein and Peptide Microanalysis Laboratory.
Immunoblot
After SDSPAGE, proteins were transferred to NC, which was blocked overnight at 4°C in TTBS (0.2% Tween-20, 10 mM TrisHCl, pH 7.5 and 0.2 M NaCl). Blots were incubated with primary antibodies [rat anti-Hsc70 monoclonal antibody (MAb), 1:2000 (StressGen Biotech- nologies Corp., BC, Canada); mouse anti-Hsp70 MAb, 1:2000 (StressGen); mouse anti-Hsp70/Hsc70 MAb, 1:2000 (Boehringr Mannheim); rabbit anti-NR2B polyclonal antibody (PAb), 1:5000 (Moon et al., 1994)] for 2 h at room temperature. Blots were rinsed in TTBS four times (20 min each) and incubated with alkaline phosphatase-conjugated secondary antibodies (Boehringer Mannheim) for 2 h. The anti-Hsc70 blot was incubated with biotinylated anti-rat immunoglobulin G (IgG; StressGen) for 2 h, rinsed with TTBS, then incubated with alkaline phosphatase- conjugated streptavidin. Blots were developed according to the supplier's instructions. For quantification, blots were scanned and the signal strengths were measured with the NIH Scion Image Beta 3b software. The solubility of each protein was expressed as mean ± SD.
Immunocytochemistry of Dissociated Hippocampal Cultures
Cultures of embryonic day 18 rat hippocampal neurons were grown as described previously (Brewer et al., 1993). After 25 weeks, cultures were fixed (Kornau et al., 1995
) and double-labeled with anti-Hsc70 (rat monoclonal SPA-815, StressGen) at 1:500 or anti-Hsp70 (mouse monoclonal SPA 810, StressGen) at 1:50 (Milarski et al., 1989
) and 10 µg/ml affinity-purified anti-PSD-95 (rabbit PAb) (Apperson et al., 1996
; Kornau et al., 1995
). After three washes in the preblock solution (Apperson et al., 1996
), coverslips for the Hsc70/PSD-95 double-label were incubated with biotinylated anti-rat IgG (Vector, 1:100) and Cy3-conjugated anti-rabbit IgG (Chemicon, diluted 1:100 in preblock), rinsed and incubated further with fluorescein-conjugated streptavidin (Vector, 1:50) in PBS (20 mM sodium phosphate buffer, pH 7.4, 450 mM NaCl and 0.05% Triton X-100) for 1 h at room temperature. Coverslips for Hsp70/PSD-95 double-label were incubated with goat anti-mouse IgG (Alexa 568, Molecular Probes, 1:100) and goat anti-rabbit IgG (Alexa 488, Molecular Probes, 1:100). Coverslips were rinsed and mounted with 4% n-propylgallate in 90% glycerol, 10% sodium carbonate buffer (pH ~8.7) and viewed in a fluorescence laser-scanning confocal microscope (Zeiss LSM310, Oberkochen, Germany). Original images were obtained with the contrast and brightness set at 401 and 9701, respectively. At these settings, there was no bleed-over from the goat anti-rabbit Alexa 488-labeled PSD-95 into the 543 laser channel and vice versa. The original images were processed with Adobe Photoshop 5.0.
Immunohistochemistry and Immunoelectron Microscopy
Male rats (Sprague-Dawley, 200250 g) were allowed free access to food and water under a 12 h light and dark cycle for a week before sacrifice. Brains were fixed with 4% paraformaldehyde in 100 mM sodium phosphate buffer (pH 7.4) by perfusion through the left heart ventricle for 15 min and immersed in the same solution for 12 h at 4°C followed by immersion in the same buffer containing 30% sucrose. Brains were flash-frozen on dry ice, embedded in O.C.T. compound (Tissue-Tek) and sectioned on a cryostat (35 µm). Sections were rinsed in 20 mM phosphate-buffered saline (PBS) three times (15 min each) at 4°C to remove O.C.T. compound. After preincubation of the sections in 10% normal goat serum (NGS) for 1 h at 4°C to block nonspecific binding, the sections were incubated with primary antibodies [rat anti-Hsc70 monoclonal (StressGen, 1:1,000) or mouse anti-Hsp70 Mabs (StressGen, 1:100) in PBS containing 1% NGS and 1% bovine serum albumin for 48 h at 4°C]. After washing in PBS, sections were incubated with secondary antibodies [biotinylated goat anti-rat IgG (Vector Laboratories, 1:500) for Hsc70 or biotinylated goat anti-mouse IgG (Vector Laboratories, 1:500) for Hsp70] in PBS for 24 h at 4°C. The sections were washed three times in PBS and incubated in an avidinbiotinperoxidase complex (ABC kit, Vector Laboratories) for 1.5 h at room temperature. After washing twice in PBS and once in 50 mM TrisHCl (pH 7.6), peroxidase was revealed by incubation (510 min) with 0.0048% H2O2 in the presence of 3,3- diaminobenzidine (DAB; 0.05% in 50 mM TrisHCl, pH 7.6). The reaction was stopped by several washes in 50 mM TrisHCl (pH 7.6).
For electron microscopic observation, HSP70 were revealed by immunoperoxidase staining as described above. The hippocampal areas were osmicated, stained en bloc in uranyl acetate and embedded in Epon resin. Semithin (1 µm) and ultrathin (60 nm) sections were prepared, contrasted with uranyl acetate (15 min) and lead citrate (8 min) at room temperature, and examined under a transmission electron microscope.
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Results |
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The One-Triton PSD fraction was isolated from rat forebrains and fractionated into 1% OG-soluble and -insoluble fractions (Moon et al., 1994). Densitometric analyses indicated that the PSD-72 protein band (Fig. 1
, PSD-72) represented ~1% of the total One-Triton PSD fraction (data not shown). The 72 kDa proteins in each fraction were electroeluted separately. Tryptic peptides of OG-insoluble PSD-72 were purified through HPLC. One large HPLC peak was successfully sequenced, producing mixed sequences. However, the major (peptide 1) and a minor (peptide 2) sequences were unambiguously identified by the size of the signals. The OG-soluble PSD-72 was fragmented by CNBr and two of the fragments were successfully sequenced (peptide sequences 3 and 4 in Fig. 1A
). All four amino acid sequences were found in those of Hsc70 (Sorger and Pelham, 1987
) and Hsp70 (Longo et al., 1993
) (Table 1
). However, the amino acid sequences were aligned better with Hsc70 (Table 1
).
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When an antibody specific for Hsp70 was used to probe a similar blot of PSD proteins, a single band appeared (Fig. 1B,
-Hsp70, arrow) at the 72 kDa position. The signal, however, was weaker than that of Hsc70 (usually the Hsc70 signals were visualized in 510 min, while that of Hsp70 appeared in 12 h), indicating that the amount of Hsp70 present in the PSD fraction is much less than that of Hsc70.
The Nature of the Association of HSP70 with the PSD Fraction
To understand the characteristics of the association of HSP70 with the PSD fraction, we extracted the One-Triton PSD fraction with various detergents or with salt. The soluble and insoluble fractions were probed for the presence of each protein with specific antibodies (Fig. 2) and the solubility for each protein was shown in Table 2
. About one-third of the lower band (72 kDa) of Hsc70 was solubilized in 0.51.0% Triton X-100 or OG, while slightly more than half of the upper band (75 kDa) of the Hsc70 was solubilized (Fig. 2A
, Hsc70, upper arrowhead). The Hsp70 showed different characteristics in solubilization from the Hsc70. Less than one-fifth of the Hsp70 was solubilized in 0.51.0% Triton X-100. However, 1.0% OG solublized 31.9 ± 6.8% (n = 4) of the Hsp70, which is significantly different from the solubility (15.0 ± 4.2%, n = 4) in 0.5% OG (MannWhitney U-test, P < 0.05). Under these conditions, solubilization of the N-methyl-D-aspartate (NMDA) receptor subunit 2B (NR2B), which is a transmembrane protein tightly bound to the PSD fraction, was minimal (Fig. 2A
, NR2B). A harsher detergent, N-lauroyl sarcosinate (sarcosyl, 3%), however, solubilized 62.9 and 57.8% of the Hsc70 and Hsp70, respectively (Fig. 2A
, sarco- syl), in which condition 48.7 ± 1.4% (n = 3) of the NR2B was also solubilized.
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To study subcellular distributions, the amounts of HSP70 present in brain homogenate, synaptosome and PSD fractions were compared. As shown in Figure 2C, Hsp70 and the lower band of Hsc70 are not specifically enriched in the PSD fraction. Interestingly, the amount of the upper band (75 kDa) of Hsc70 is dramatically reduced (14.1 ± 2.2%, n = 2, that of synaptosome) in the One-Triton PSD fraction (Fig. 2C
, Hsc70, upper arrowhead), indicating that the majority of this protein is removed by 0.5% Triton X-100 and that it may not be an intrinsic member of the PSD. The tissue distribution of the two Hsc70 proteins was also investigated through immunoblot analyses of several tissue homogenates. As shown in Fig. 2D
, the spinal cord and thymus were similar to the brain in the relative amounts of the two Hsc70 proteins. In all other tissues tested, i.e. liver, kidney, muscle, testes, pancreas, heart and lung, the expression levels of the upper band (75 kDa) of Hsc70 were higher than those of the lower one (72 kDa). Interestingly, the expression levels of the upper band of Hsc70 were very high in the liver, kidney, pancreas, and heart. Moreover, there was an additional band in the liver and kidney (Fig. 2D
, bars), indicating that there is another isoform of Hsc70 in these tissues.
Expression of HSP70 in Rat Cerebral Cortex and Hippocampal Formation at the Light Microscopic Level
The presence of HSP70 in the PSD fraction prompted us to com- pare the expression of Hsc70 and Hsp70 in vivo with specific antibodies. In general, immunoreactivities of both proteins were distributed throughout the brain (Figs 3 and 4), although staining for Hsc70 was stronger. As expected from the immunoblot analyses, the immunoreactivity of Hsp70 was weak in all regions of the forebrain, but the signals were definitely above the back- ground (Fig. 3
). In the cerebral cortex, pyramidal neurons were positively stained (Fig. 3
, Cx). Perikarya and dendrites were also stained. Staining of nucleoplasm was not evident, but nucleoli were stained almost as strongly as perikarya (Fig. 3
, Cx, upper right panel). In the hippocampal formation, perikarya of pyra- midal cells in cornus ammons areas 13 (CA13) (Fig. 3
, CA1, upper right panel), polymorphic neurons in CA4 (Fig. 3
, CA4, inset) and granule cells in the dentate gyrus (DG) (Fig. 3
, DG, inset) were stained. Staining of dendrites was also evident in CA1 pyramidal cells (Fig. 3
, CA1, lower right panel) but was not in cells in CA4 and DG (Fig. 3
, insets of CA4 and DG). In control images, for which the primary antibody was omitted, no signals were detected even at higher contrasts (Fig. 3
, Control-Cx and Control-HC, insets).
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Immunocytochemical Localization of HSP70 in Dissociated Hippocampal Neurons
Biochemical and histological evidence for the presence of HSP70 in brain neurons encouraged us to perform immunocytochem- ical localization of the two proteins in dissociated hippocampal neurons. Hippocampal neurons, dissociated at embryonic day 18, were double-stained with antibodies specific for one of the HSP70 and PSD-95 proteins. The latter is known to be highly localized at the PSD in the forebrain (Kornau et al., 1995; Hunt et al., 1996
). Confocal imaging revealed that the Hsp70 immunoreactivity was distributed throughout the neuron (Fig. 5
). Immunoreactivity was stronger in perikarya and proximal dendrites than in distal processes, and was not uniform but punctate in both perikarya and processes. Staining intensity varied significantly among dendritic puncta and protrusions in 2-week-old neurons (Fig. 5A
, upper right panel), while the intensities of punctate staining for PSD-95 were relatively uniform (Fig. 5A
, lower right panel). When the two images were superimposed, most of the punctate staining overlapped (Fig.5A, left panel, yellow color), indicating that the two proteins are mostly co-localized. There were, however, many puncta which were apparently labeled only with Hsp70 (green color, arrowheads in the insets of Fig. 5A
, left panel), but pure red puncta was rare and most of the apparently red puncta actually had yellow hues (Fig. 5A
, inset a, arrows) in 2-week-old cultures. Interestingly, when 5
-week-old cultures were double- stained, almost all of the dendritic puncta and protrusions were superimposed (Fig. 5B
). Occasionally, however, dendritic protrusions labeled with only the Hsp70 antibody were found (Fig. 5B
, left panel, large arrow and arrowhead in the inset). Moreover, variations in the staining intensities were small (see the yellow color of uniform intensities in most of the double-labeled puncta in Fig. 5B
, left panel), suggesting that the amount of Hsp70 in the synapse stabilizes with synaptic maturation. A control image, which was single-labeled with the Hsp70 antibody (Fig. 5C
, left panel), was very similar to the double-labeled ones, indicating that images were not altered by double-labeling. In another control, in which primary antibodies were omitted, no signals were detected in processes and only very weak signals were associated with perikarya (Fig. 5C
, right panel, arrows). There was absolutely no bleed-over of signals between the two light channels during the recording of images by confocal microscopy (not shown).
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Synaptic localization of HSP70 proteins in the adult rat hippocamal neurons was further studied at the immunoelectron microscopic level. The most immunoreactive structures for Hsp70 in synapses were the asymmetrical PSDs (Fig. 7, asterisk), some of which were axospinous synapses (Fig. 7C
, asterisk). Presynaptic thickenings and synaptic vesicles were also stained, but less strongly than the PSD. Patch-like staining of cytoplasmic matrix was also detected. Symmetric synapses were stained very weakly (Fig. 7B,C
, large open arrows). The DAB reaction products were also associated with some mitochondria (Fig. 7A
, small open arrow), but other mitochondria were stained very weakly (Fig. 7A
, small solid arrow). The DAB reaction product associated with mitochondria may be an artifact, as mito- chondria frequently contain a high level of free radicals.
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Discussion |
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Presence of both Hsp70 and Hsc70 in the PSD
The Hsp70 and Hsc70 are very similar in amino acid sequence (~90% identity) and molecular size (O'Malley et al., 1985). Although the immunoblot signals for Hsp70 in the forebrain PSD fraction were weak compared with those for Hsc70, we argue, for several reasons, that the presence of Hsp70 in normal brain synapses is real and that the Hsp70 signals seen in this study are not from cross-reaction with Hsc70. First, the binding strength of Hsp70 with the PSD is larger than that of Hsc70 (Fig. 2A
). Second, the amount of Hsp70 associated with the One-Triton PSD fraction is not reduced compared with that in brain homogenate or in synaptosomal fractions (Fig. 2C
), indicating strong association of the Hsp70 with the PSD. Third, although the signal from immunohistochemical staining for Hsp70 is much weaker than that for Hsc70, it is above the background level in forebrain neurons (Figs 3 and 4
). Fourth, confocal images of immunostained hippocampal neurons show that Hsp70 is associated with spine-like structures or dendritic protrusions, and that the pattern of punctate staining is different from that of Hsc70 in regard to variation in signal intensities and co-local- ization with PSD-95 (Figs 5 and 6
). Last, both the Hsp70 and Hsc70 immunoperoxidase reaction products were associated primarily with the PSD but only Hsc70 was associated with the amorphous subsynaptic structures and spine apparatus-like structures (Figs 7 and 8
). Together with the finding that three out of four amino acid sequences from the 72 kDa band were aligned better to Hsc70 than to Hsp70 (Table 1
), we conclude that both Hsp70 and Hsc70 are present in the PSD and that the major HSP70 associated with the forebrain PSD is Hsc70. Our data are inconsistent with a recent report by Suzuki et al. (Suzuki et al., 1999
), who reported that only Hsc70, but not Hsp70, is present in the PSD fraction.
Biochemical Characteristics of HSP70 (Hsc70 and Hsp70) Associated with the PSD
The PSD is thought to be composed of highly insoluble core proteins and other proteins which are associated with the core (Kennedy, 1993, 1997
). Proteins tightly bound to the PSD core are insoluble in mild detergents such as OG or Triton X-100 (Apperson et al., 1996
). The fact that PSD-associated HSP70 proteins were efficiently dissociated from the PSD core by salt (1.0 M NaCl) (Fig. 2B
), but not by OG (0.51.0%) or Triton X-100 (0.51.0%) (Fig. 2A
), indicates that both Hsp70 and Hsc70 are closely associated with the PSD core mainly through hydro- philic protein interactions. In contrast to Hsc70, however, the solubility of Hsp70 is increased about twofold (from 15.0 to 31.9%) in 1.0% OG compared with 0.5% OG, suggesting a slight difference in binding strength or characteristics of the two proteins with the PSD core. The HSP70 proteins are not enriched in the PSD (Fig. 2C
). This may be due to the fact that the HSP70 proteins are also distributed in somata, dendrites and spines (Figs 36
), and that not all PSDs contain HSP70 proteins (Figs 7 and 8
). We did not address the cognate proteins that associate with the HSP70 proteins. However, the HSP70 contain a highly conserved calmodulin-binding domain (Stevenson and Calderwood, 1990
). Since calmodulin is a major PSD protein that binds to a number of proteins there (Grab et al., 1979
; Carlin et al., 1981
), HSP70 proteins may be associated with calmodulin in the PSD.
Association of the 75 kDa Hsc70 with the PSD is unlikely, because the amount of 75 kDa Hsc70 was dramatically reduced in the PSD compared woth the synaptosome fraction (Fig. 2C). Instead, the 75 kDa Hsc70 may be the one associated with the amorphous subsynaptic structures or spine apparatus-like cisternae seen in electron microscopic studies (Fig. 8
). This possibility is further supported by reports that a constitutive member of the HSP70 family with a molecular size of 74 kDa has been localized to various membranous organelles (VanBuskirk et al., 1991
; Domanico et al., 1993
; Bhattacharyya et al., 1995
).
Differential Distribution of HSP70 at Synaptic Sites
Our immunoelectron and confocal microscopic studies have shown differential distribution of the HSP70 proteins at synaptic sites. Only Hsc70 was associated with spine apparatus-like cisternae and unidentified amorphous subsynaptic structures (Figs 7 and 8). The latter may be the subsynaptic webs which have been previously described as associated with Hsc70 (Suzuki et al., 1999
). At the confocal microscopic level, the Hsp70 was frequently not co-localized with PSD-95 at puncta or spine-like protrusions of dissociated hippocampal neurons, and variations in amount among these structures were large in young cultures (Fig. 5A
). Interestingly, most of the Hsp70 became co-localized with PSD-95 in older cultures (Fig. 5B
), suggesting that Hsp70 may be involved in an early stage of synaptic development, such as formation of new dendritic sproutings, in addition to synaptic maturation and/or activity-dependent modulation of synaptic strength.
Possible Roles of PSD-associated HSP70
The most straightforward interpretation of the function of the HSP70 proteins in the cytoplasm of synaptic sites and dendritic shafts would be that they are involved in facilitation of folding of nascent proteins and in the repair of partially denatured proteins. Rapid input-specific growth of small filopodia-like protrusions (Maletic-Savatic et al., 1999) and formation of new spines (Hosokawa et al., 1995
; Engert and Bonhoeffer, 1999
) are induced by synaptic stimulation. Recently, tetanic stimulation has been shown to cause local protein synthesis in dendrites of hippocampal CA1 pyramidal neurons (Ouyang et al., 1999
) and dentate granule cells (Steward and Halpain, 1999
). Therefore, HSP70 proteins may function in the process of local synthesis of new proteins required for synaptic plasticity, remodeling, neurite outgrowth and/or the stabilization of existing or nascent synapses.
Hsc70 may function in clathrin-dependent endocytosis. The clathrin-mediated synaptic vesicle endocytosis (Gad et al., 1998; Palfrey and Artalejo, 1998
) and postsynaptic receptor internal- ization (Craven and Bredt, 2000
; Man et al., 2000
; Wang and Linden, 2000
) have been reported. With a clathrin-uncoating ATPase activity (Rothman and Schmid, 1986
; DeLuca-Flaherty et al., 1990
), Hsc70 may participate in vesicle trafficking in both pre- and postsynaptic compartments.
The HSP70 associated with the PSD may function in a pro- tein holding and folding system as part of a synaptic tag. A potential synaptic tag would be capable of both sequestering the plasticity proteins in stable form and releasing and/or activating them upon synaptic stimulation (Frey and Morris, 1997, 1998
). Since the HSP70 proteins can act as both a holding and a folding system depending on the chaperone cofactors associated with them (Zeiner et al., 1997
; Bimston et al., 1998
; Takayama et al., 1999
), they would be very good candidates for part of a synaptic tag.
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Notes |
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Address correspondence to Il Soo Moon, Department of anatomy, College of Medicine, Dongguk University, 707 Sukjang, Kyongju 780-714, Korea. Email: moonis{at}mail.dongguk.ac.kr.
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Footnotes |
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