Biotransformation of the tobacco-specific carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in freshly isolated human lung cells

The publishers wish to apologise for errors which occurred in the above paper, as follows:

On p. 1811, on the 2nd line under the heading Incubations with NNK and in the second sentence under the heading Measurement of NNK biotransformation, the Greek mu characters have been incorrectly changed to `m'. The correct sentences are printed below.

Immediately following isolation, lung cells were incubated in sterile 24-well Nunclon {triangleup} polystyrene multidish plates with 4.2 µM (10 µCi) [5-3H]NNK (>98% purity) in 1 ml RPMI-1640 medium, supplemented with antibiotic-antimycotic (1 ml/l), for 24 h at 37°C under 95% air–5% CO2.

Briefly, 500 µl of filtered (0.45 µm polypropylene, Millipore) incubation medium plus 5 µl of NNK metabolite reference standards (UV markers) were co-injected onto a Waters µBondapak C18 column.

Notes

by Graeme B.J.Smith, Andre Castonguay, Patty J.Donnelly, Ken R.Reid, Dimitri Petsikas and Thomas E.Massey





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