Department of Industrial Hygiene and Toxicology, Finnish Institute of Occupational Health, Helsinki,
1 Department of Oncology,
2 Department of Surgery,
3 Department of Clinical Pathology and Forensic Medicine and
5 Department of Clinical Nutrition, University of Kuopio, Kuopio, Finland and
4 Unit of Cancer Epidemiology (INSERM U521), Gustave-Roussy Institute, Villejuif, France
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Abstract |
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Abbreviations: MnSOD, manganese superoxide dismutase; MTS, mitochondrial targeting sequence; OR, odds ratio; RFLP, restriction fragment length polymorphism; ROS, reactive oxygen species.
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Introduction |
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Superoxide dismutases play a key role in the detoxification of superoxide radicals (O2 ) thereby protecting cells from damage induced by free radicals (3). Within mitochondria manganese superoxide dismutase (MnSOD) provides a major defence against oxidative damage by ROS. It is synthesized in the cytosol and post-transcriptionally modified for transport into the mitochondrion (4). A variety of cancer cells are known to express reduced levels of antioxidant enzymes, especially MnSOD, when compared with their normal counterpart (5,6). Furthermore, the increased expression of MnSOD has been found to suppress the malignant phenotype of human breast cancer cells suggesting that MnSOD is a tumour suppressor gene in human breast cancer (7).
Mitochondrial targeting sequence (MTS) readily forms an amphiphilic helical structure, which is crucial for effective transport and processing of mitochondrial proteins. A one base pair transition (TC) leads to a Val
Ala amino acid change at 9 position of MTS (8). This change, the prevalence of which has been found to differ among various ethnic groups (9), may result in a `disease of distribution' where allocation rather than activity of an essential protein is faulty (10). In agreement with this, the MnSOD AA genotype has been associated with increased breast cancer risk, especially among pre-menopausal women with a low consumption of dietary sources of antioxidants (11).
This study, which is an extension of the Kuopio Breast Cancer Study (12), was undertaken to examine the role of the MnSOD genotype further in a Finnish Caucasian study population.
The cases consisted of women who had a suspicious breast lump or breast symptoms and lived in the catchment area of Kuopio University Hospital during the study period (from 1990 to 1995). They were referred by a physician for further examination, and were asked to participate in the study at the first hospital visit. Detailed data concerning socio- economic background, reproductive history, medical history, family history of breast cancer, current alcohol intake, smoking and body-size indicators were recorded during an interview with a study nurse, completed before any diagnostic procedures had begun (12). A total of 516 women were finally diagnosed with histologically confirmed breast cancer. Only 12 women later diagnosed with breast cancer refused to participate. The final participation rate for the cases was therefore 98%.
Lymphocyte DNA was available for 486 breast cancer patients. Three of the patients were excluded from the study because of poor quality of the sample DNA. Thus the final patient population included 483 incident breast cancer cases (44.391.6 years of age, mean 58.9 years).
Healthy population controls with no breast symptoms or previous breast problems and living in the study catchment area were drawn from the Finnish National Population Register. In all, 514 controls were interviewed in parallel with the cases, the final participation rate being 72%. Of these, lymphocyte DNA was available for 492 subjects. Four population controls having earlier breast cancer diagnosis, two controls of non-Finnish origin and four controls whose DNA was of poor quality were excluded from the study. Therefore, the final study population included 482 controls (37.577.2 years of age, mean 53.5 years).
Lymphocyte DNA (100 ng) extracted by standard techniques was used as a template in PCR-based restriction fragment length polymorphism (RFLP) assays performed essentially as described earlier (11). Briefly, the 107 bp PCR product was amplified using specific primers (5'-ACC AGC AGG CAG CTG GCG CCG G-3' and 5'-GCG TTG ATG TGA GGT TCC AG-3'), which create a restriction site for NgoMIV (New England Biolabs) in the MnSOD A allele resulting in restriction fragments of 89 and 18 bp. The uncut 107 bp fragment identified the MnSOD V allele.
Positive and negative controls were used within each batch of PCR. The experimenter was unaware of each sample's case-control status. Two independent readers interpreted the RFLP results. All samples with ambiguous results, and a random selection of 10% of all samples, were repeated to ensure laboratory quality control. Because of unreadable genotype data for one control and three cases, results are presented for 482 controls and 479 cases.
Age-adjusted odds ratios (ORs) and 95% confidence intervals (CIs) were calculated by unconditional logistic regression. Women who reported natural menopause or had undergone bilateral oophorectomy were classified post- menopausal. Hysterectomized women with intact ovaries (ovary) (40 cases and 41 controls) and women for whom the details of the operation were unknown (six cases and two controls) were also classified post-menopausal if they were no longer menstruating and were older than 51 years (median for menopause in Finnish women). All the others were classified as pre-menopausal.
Interactions between MnSOD genotype and smoking, use of alcohol, use of oral contraceptives, post-menopausal use of oestrogen or use of supplement vitamins were assessed by the likelihood tests to compare the goodness of the fit of the models with and without the interaction term. Subjects who had in the past smoked daily for at least 3 months were considered as smokers. Ever smokers (ex- or current at the reference date) were further categorized to two levels of smoking exposure according to the approximate median value for smoking years (15 years) and daily consumption (10 cigarettes) in the control population. Daily tobacco consumption was similar in cases and controls (11.2 cigarettes/day versus 9.5 cigarettes/day, P = 0.099) as was the duration of smoking (16.1 years versus 14.5 years, P = 0.250).
Women who reported taking vitamin supplements (A, C or E vitamins) at least three times per week for longer than 6 months were considered as users of vitamin supplements. Average daily consumption of tobacco, use of oral contraceptives and use of post-menopausal oestrogen were considered as categorical or continuous variables.
Individuals carrying the homozygous MnSOD VV genotype served as a reference category for all separate analysis of the genotype. All reported P values were two-sided, and a result was considered significant when P < 0.05.
In a previous study, we found waist to hip ratio, first degree family history of breast cancer and history of benign breast disease were positively associated with breast cancer risk, whereas parity and use of oral contraceptives showed an inverse association compared with women without these risk factors (13).
The genotype distributions in the control population were in agreement with those predicted under the conditions of HardyWeinberg equilibrium (P = 0.652). A 1.5-fold (95% CI 1.12.0) risk of breast cancer was found for individuals carrying the MnSOD A allele-containing genotypes compared with the MnSOD VV genotype (Table I). A similar risk approaching statistical significance was also observed in the sole previous study on breast cancer risk and MnSOD polymorphism (OR 1.8, 95% CI 0.93.6) (11). The most remarkable risk in that study, however, was associated with the MnSOD AA genotype compared with the VV genotype (OR 4.3, 95% CI 1.710.8) in pre-menopausal women. In our study, this comparison revealed no statistically significant association (OR 1.4, 95% CI 0.72.5), possibly because of lack of statistical power.
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When the risk associated with the MnSOD A allele was studied stratified by factors suspected to have a role in the production of ROS or in their detoxification, no significant associations were seen when pre- and post-menopausal women were considered together. However, after stratification by menopause, significant interaction was found between the MnSOD genotypes and post-menopausal use of oestrogen; women who had used oestrogen replacement therapy and carrying a MnSOD A allele containing genotypes had a 2.5-fold higher risk of breast cancer (95% CI, 1.34.8; P for interaction 0.02) (Table II).
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Similarly, in post-menopausal women the effect of MnSOD A allele-containing genotypes was more pronounced among current users of alcohol and women who had in the past used oral contraceptives (Table II). These associations were not modified by the duration of use.
The strengths of this study are its relatively large size, genetic homogeneity of the study population, and good background information on both reproductive history and many life style factors, whereas one of its limitations might be the absence of information on dietary habits of the study subjects, restricting us from assessing the risk modification by diet, as was done by Ambrosone et al. (11). However, we could examine the effect of intake of vitamin supplements as was also done in the above study (11), where pre-menopausal women not taking vitamin C or -tocopherol were shown to be at increased risks. We could not, however, see any effect when the use of A, C or E vitamins were considered together. This might be explained, at least in part, by the low numbers of subjects using these vitamins.
It should also be noted that all analyses stratified by menopausal status were based on substantially lower numbers of subjects in each strata, lowering the power of the analyses. This might at least partly explain why most statistically significant associations were seen in subjects with heterozygous MnSOD VA genotype, while no trend of increasing risk was seen among subjects carrying the MnSOD AA genotype. It may also be one explanation for the lack of any significant associations among the pre-menopausal women. Further studies with larger sample sizes to offer sufficient statistical power also for the subgroup analyses are therefore evidently needed before any strict conclusions can be drawn on this issue.
According to this hypothesis, the polymorphism in MTS of MnSOD affects the mitochondrial targeting of the enzyme, modifying the ability of mitochondria to defend against oxidative stress by ROS. However, the amphiphilic helical structure essential for correct transport is predicted for the MnSOD A allele, which was the risk allele both in this and in the previous study by Ambrosone et al. (11). The possible explanation for the negative role of the MnSOD A allele may be the simultaneous accumulation of H2O2, at least in the absence of H2O2 scavenging enzymes such as glutathione peroxidase and catalase. In the presence of transition metals, this in turn gives rise to generation of potent free radicals and hence mitochondrial damage. Furthermore, as discussed by Ambrosone et al. (11), the prediction of an altered cellular location of the MnSOD is based on a limited statistical model and should thus be interpreted with caution.
To conclude, although no strict conclusions can be drawn before real data on the biological consequences of the MnSOD variants is available, our results support the hypothesis that MnSOD genotypes may modify individual breast cancer risk. This remains to be corroborated in future studies.
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Notes |
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Acknowledgments |
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References |
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