1Faculty of Psychology and 2Department of Psychiatry and Neuropsychology, Maastricht Brain & Behaviour Institute, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands*Corresponding author: Faculty of Psychology, Section Neurocognition, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands
Accepted for publication: July 7, 2001
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Abstract |
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Br J Anaesth 2001; 87: 7813
Keywords: age factors; risk; cognition
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Introduction |
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The many, uncontrollable variables in human subjects make appropriate studies difficult and an animal model would be helpful. We describe a longitudinal experiment in which the effects of repeated anaesthesia on reaction time were examined in rats. We repeatedly anaesthetized and tested their cognitive performance with a choice reaction time (CRT) task throughout life. The study continued until the rats were 26 months old, when rats may be considered aged. If anaesthesia is a vulnerability factor for cognitive ageing, the performance of the experimental groups should diverge in the course of their lifespan.
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Methods and results |
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Experimental design
At the age of 68 months, the rats were trained on the CRT task7 after food deprivation to 85% of free feeding weight, matched for their reaction time performance and assigned to the control (n=12) or treatment (n=15) group. Rats in the treatment group were then anaesthetized and the CRT was repeated 2 days later. Free feeding was then permitted until the next test session. The control group was treated and tested identically apart from the anaesthesia. Thereafter, the sequence of food deprivation, training, CRT, anaesthesia and CRT was repeated at intervals of 2.5 months, when the rats were 6, 8.5, 11, 13.5, 16, 18.5, 21 and 23.5 months old. Two months after the last study period the CRT was repeated for the last time, as the number of rats that responded reliably in the behavioural task was reduced to eight and nine in the control and treatment groups respectively. After the final behavioural test, when the rats were 26 months old, the animals were killed and the brain was removed for biochemical analysis.
Anaesthesia
Sodium pentobarbital (20 mg kg1 in 0.3 ml) was administered by i.p. injection. Qualitative observations indicated that the animals lost their reflexes and were unresponsive to gentle stimuli for about 23 h. No surgery or painful stimuli were administered. During the period of anaesthesia the rats were placed in an incubator held at a constant 32°C. When the rats had recovered, they were returned to their home cage. Control rats were not injected.
Statistical analysis
All data were compared by analysis of variance with two factors (treatment and age), with age as a repeated-measures factor. Group differences at individual time points were evaluated using the t-test.
Mortality
Four control rats and four rats from the anaesthetic group died for unknown reasons. Of the remaining rats, two rats in the anaesthetic group did not complete sufficient trials in the behavioural tasks and were excluded from the statistical analyses.
Reaction time performance
Reaction time
Figure 1A shows the change in reaction time with age. The rats responded faster with age up to the age of 18.5 months [age, F(5,75)=6.89, P<0.01], and this was similar for the two experimental groups [treatment x age, F(5,75)=1.55, not significant]. Individual t-tests revealed a tendency for a faster mean reaction time in the treated group in the last two test sessions (t<1.96, 0.05<P<0.10).
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Premature responses
The development of premature responses is shown in Fig. 1C. There were no statistically reliable differences between the groups for the first six test sessions (treatment x age, treatment F<1.32, not significant). However, for the last three test sessions there was a tendency for treated rats to make more premature responses than control rats [treatment, F(1,15)=3.64, 0.05<P<0.10], which was due mainly to the treatment effect in the last test session [t(15)=2.79, P<0.05].
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Comment |
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Previous human studies that have shown neurological changes have noted that the observed effects may have resulted from either the anaesthetic or the accompanying surgery and the associated stress response or both.1 No surgery was conducted on the animals in the present study and, while we cannot exclude a degree of stress, the likely cause of the observed changes was the pentobarbital.
It remains to be determined whether the effects of treatment in the present study were related to an acute effect of pentobarbital in old rats or were the result of a cumulative effect. Acute thiopental treatment did not affect the spatial memory performance in young and old rats (given the same dose of thiopental), which does not support the concept that the effects in the present study were an acute effect of treatment.9 Moreover, in the present study the rats were not anaesthetized before the final behavioural test, when behaviour was most affected, strongly suggesting that the effects of anaesthesia are long-lasting or cumulative.
In summary, our findings suggest that repeated anaesthesia affects the behaviour of rats in the later stages of life through an increase in impulsivity. The findings support observations in studies with healthy old people10 and aged patients15 and supports the hypothesis that repeated anaesthesia is a vulnerability factor for cognitive ageing. Further studies examining the effects of different anaesthetic agents and different patterns of administration in additional animal models are indicated.
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Acknowledgements |
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References |
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