1 University Department of Anaesthesia, Level 4, Addenbrookes Hospital, Cambridge UK. 2 Physiological Laboratory, University of Cambridge, Cambridge CB2 3EG, UK
Corresponding author. E-mail: rhsc1@cam.ac.uk
Accepted for publication: March 29, 2003
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Abstract |
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Methods. We tested the effects of random doses of 0, 0.1, 0.2 and 0.3 MAC sevoflurane in six subjects, in a double-blind study using two measures of behavioural impairment: saccadic latency and stop signal reaction time (SSRT) in a countermanding task.
Results. Saccadic latency and SSRT both increased with increasing doses of sevoflurane. In both measures, reciprocal reaction time was linearly related to dose in each subject: all but two of the twelve regression coefficients were statistically significant (P<0.05). In one subject, SSRT was significantly more sensitive than simple latency (P<0.05); for the others there was no significant difference.
Conclusion. Measurements of this kind could potentially provide estimates of cortical effects of sevoflurane sedation, and give a clinically useful measure of cognitive fitness.
Br J Anaesth 2003; 91: 17583
Keywords: anaesthetics volatile, sevoflurane; complications, saccadic latency; recovery; sedation
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Introduction |
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The neural mechanisms controlling saccades extend from the brainstem, which determines the amplitude and velocity of movements, to the cortical frontal eye fields, which are involved in the decisions to initiate movements and timing of saccades (latency), and in decisions to withhold or change a previously planned movement (countermanding).
In principle, saccadic eye movements could be used to indicate anaesthetic effects at two levels: i) the brainstem, containing the neural circuits that control saccadic velocity and amplitude; this can be measured by peak saccadic velocity (PSV); ii) the cortex, including frontal cortical areas implicated in decision making and saccadic initiation;7 8 this can be measured by either saccadic latency or the stop signal reaction time (SSRT).9 10 The latter uses a countermanding task to assess the even higher cortical ability to change or stop an already planned action.
PSV has been used widely to measure brainstem depression by anaesthetics.1113 In the 0.050.1 MAC range, there is a dose-related effect, allowing a sensitive index of sedation.1417 Latency and similar measures of cortical decision-making function do not indicate the concentration of anaesthetic agent per se, but the actual effect on cognitive fitness, which in the end is what matters. For example, a patient who ignored medical advice and drove a car home after a general anaesthetic might need to apply the brakes suddenly a decision in response to a child stepping onto the road. In contrast to saccadic velocity, the effects of anaesthetics on saccadic latency and SSRT are not well studied. Both latency and SSRT18 were increased by isoflurane 0.15% (0.1 MAC) in one study; in another, saccadic velocity and latency both changed 1 h after midazolam.19 A third study suggested that latency is more sensitive to sedation than saccadic velocity: 4 h after diazepam or thiopental, only latency was increased, PSV having returned to the control value.20 However, the latter two studies used about 30 trials to measure latency,19 20 an insufficient sample given the intrinsic variability of oculomotor latency.
We measured the dose response of saccadic latency and SSRT to sub-anaesthetic sevoflurane in order to assess the effects on cortical decision making, using enough trials (2000 for each subject) to carry out distributional and other kinds of quantitative analysis.
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Methods |
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Oculometry
We measured eye movements using a head-mounted scleral reflection infrared oculometer2 in the form of goggles having a range of ±30° and symmetrically linear to 1% over about ±10°. Output was sampled at 10-ms intervals with a PC-based saccadic analysis system (Saccadic Programming and Instrumentation Computer)6 which displayed and stored eye-movement data, controlled the stimuli and detected saccades in real time with an algorithm using acceleration, speed and position. The computer ignored saccadic latencies of less than 50 ms. The traces were inspected after each session and records with errors caused by blinks, head movements or other artefacts (typically less than 2% of the total) were eliminated from further analysis.
Stimuli
The stimuli were three red spots projected in the horizontal plane at different times onto a screen approximately 2 m from the subject by low-voltage laser diodes mounted on the goggles, which also housed the oculometer. Because the target positions were thus essentially fixed relative to the head, for moderate head movements the subjects head did not have to be immobilized, evidently a clinical advantage. The spots subtended a diameter of 26 min arc and were positioned horizontally 12° apart with a luminance of 13.2 cd m2.
Saccadic latency and the countermanding saccadic task
An experimental run consisted of a sequence of consecutive target presentations or trials. Most trials were control trials, and began with presentation of the central target, which the subject fixated. After a random interval of 0.51.5 s (to discourage prediction), the target then jumped 12° unpredictably to the left or right, and the subject tracked it with a saccade. Detection by the computer of this saccade ended the trial, and the next trial started after 100 ms. However, in a certain proportion of trials (stop trials), the central fixation spot reappeared 50 ms (the stop-signal delay) after the presentation of the lateral target, indicating to the subject not to make a saccade (Fig. 1). Control and stop trials were randomly interleaved in the ratio 4:1. Two hundred trials were presented for each dose of sevoflurane. In control trials the subject always made a saccade to fixate the peripheral target, whereas in stop trials the movement was sometimes inhibited successfully, sometimes not. The proportion, P, of stop trials on which a saccade was made, despite the countermanding signal, was recorded. Subjects became accustomed to the protocol quite quickly, with latencies becoming stable after some 30 trials; results from these learning trials were discarded.
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SSRT = T 50.(1)
The SSRTs calculated in this way are in effect mean values whose SEs can be obtained from an estimate of the SE of the corresponding value of P, which in turn can be calculated assuming a binomial distribution of uninhibited saccades. From this the corresponding fiducial limits for the SSRT can be derived using the cumulative distributions of latencies, providing a value for the SE at time T. This value also represents the SE of the SSRT since it is related to T by a constant difference (see Equation 1). The statistical significance of the difference between the two median latencies or mean SSRT values obtained for a subject in a particular pair of conditions was assessed using a standard comparison of means test to compute a t statistic.24
Anaesthetic system
The subjects were seated during the experiments. They breathed either pure oxygen or a mixture of sevoflurane and oxygen, delivered by a standard Boyles anaesthetic machine through a Bain system attached to a physiological mouthpiece. Each subject wore a nose clip with end-tidal carbon dioxide (FE'CO2) and sevoflurane concentration sampled at the mouthpiece using a Datex Capnomac Ultima (Datex Instrumentarium, Helsinki, Finland). FE'CO2 was 4.55 kPa. The machine was calibrated using the manufacturers known gas samples, as described previously.25 Pilot studies using the same study plan as the main study showed that breathing pure oxygen rather than air did not have a confounding effect. For each subject, end-tidal concentrations of sevoflurane which corresponded to 0, 0.1, 0.2 and 0.3 MAC when corrected for the age of the subject were used.26 When doses were changed the end-tidal sevoflurane was equilibrated without over-pressure, using an equilibration period of 10 min.
Experimental protocol
Each subject completed a total of 10 runs each of 200 trials in two study days. On each of the 10 runs each subject breathed one of the four doses of sevoflurane, so that each dose was breathed on two occasions, with two further zero-dose runs to make up the ten runs. The order of administration of doses was essentially random, but the first dose was always 0 MAC to establish a baseline. Subsequent experimental doses were always the same as or greater than the previous one, to avoid the effect of residual anaesthetic levels on subsequent blocks (data from the two extra 0-MAC trials were always discarded). For example, on the first day a subject might breathe 0 then 0.1 then 0.2 then 0.3 then 0.3 MAC again, and on the next day 0 then 0 then 0.1 then 0.2 then 0 MAC, the data from the final trial being discarded.
Both the subject and the experimenter were masked to the anaesthetic dose. Sevoflurane was chosen because in low doses, and when the subject is wearing a nose clip, it cannot be smelled or tasted. The same 10-min period of equilibration was used even when no anaesthetic was actually being administered, so that the subjects could not guess whether they were receiving the anaesthetic. They showed no signs of either sedation or excitement.
To ensure that the experimenters did not know the dose being given, the instruction cards for the anaesthetist were prepared before the experiments and were sealed in envelopes. Each run of 200 trials, corresponding to one distinct dose, was stored in one computer file with a randomised name; only after the analysis was complete were the doses of anaesthetic matched to each record.
Statistical analysis
Cumulative latency distributions should conform to a straight line on a reciprobit plot8 (Fig. 2). Comparisons of subjects distributions with the expected recinormals were made using the KolmogorovSmirnov statistic.27 Median saccadic latency and SE were calculated for each subject and condition, as were SSRT and its SE using the method explained above, using the percentage of saccades that were not inhibited in the stop trials. We used Origin (Microcal Software Inc., Northampton, MA, USA) to correlate the dose dependence of saccadic latency and SSRT for each subject.
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Results |
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Discussion |
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The SSRT from the countermanding paradigm9 10 23 24 measures an individuals capacity to stop an already planned action and implies a much more complex series of neuronal processes, almost certainly in the cortex. This suggested that the SSRT might be more susceptible to the effects of anaesthetic sedation than saccadic latency, and perhaps be a clinically relevant measure of whether patients can be discharged safely after day-care general anaesthesia. In terms of LATER, countermanding tasks can be considered a race between competing signals, one representing the go signal and one representing stop. If both are subject to random variation of rate, then on some trials the stop signal may reach threshold before the go signal, and therefore inhibit the response, whereas in other trials it may not. This kind of competition can be observed in the activity of cortical neurones in monkeys carrying out countermanding tasks.10 The go signal activates gaze-shift neurones which progressively increase their activity in ramp-like fashion (LATER model) and at the same time the gaze-hold neurones show a comparable but progressive reduction in activity. In the absence of a stop signal, gaze-shift activation increases until the threshold for activating a saccade is reached and the eyes move. However, when the stop signal appears 50 ms after the go signal there is a much more rapid increase in activation of the gaze-hold neurones and the gaze-shift activation falls back to baseline.
Curiously, a number of our subjects commented that the SSRT task seemed easier to perform during anaesthetic administration. Only one subject showed a significant difference between the slopes of the latencydose curve and the SSRTdose curve, with SSRT being more sensitive. Nevertheless all these data show a correlation between anaesthetic dose and saccadic latency and with SSRT. This implies a dose-dependent effect of the drug on these two variables, suggesting that these can be used to assess functional recovery from anaesthesia.
The increase in latency with sevoflurane sedation is consistent with the results obtained in a previous study on isoflurane18 and other classes of sedative agents. After midazolam, saccadic latency increased and PSV decreased appropriately.19 Padoan and colleagues20 found that an increased latency persisted for longer after thiopental or diazepam than the decrease in saccadic velocity, but Paut and colleagues19 found that the changes in PSV were significantly different from baseline for longer than latency (180 min for PSV vs 120 min for latency). The limited number of trials (six runs of 30 trials) in each of their subjects, and the use of electroculography, meant that latency could not be determined with the precision that we could obtain in our study by having 2000 trials for each subject.
PSV may give a functional measure of levels of anaesthetic agent; 0.05 and 0.1 MAC concentrations of several agents, and benzodiazepines and propofol all depress PSV in a dose-dependent manner.1113 PSV is determined by the amplitude of the pulse of activity in burst neurones in the brainstem (in the pre-pontine reticular formation and mesencephalic reticular formation, regions described as the pontine gaze centre). Via a neuronal integrator, these units drive motor neurones which, in turn, activate the extraocular muscles. Thus, while PSV is a quantitative measure of the functional behaviour of these brainstem mechanisms, it is only an indirect way of estimating any impairment of higher cortical functions. As argued above, we propose that saccadic latency may be a more appropriate measure of sedation in certain situations. Saccadic latency can be measured non-invasively and essentially automatically in a matter of minutes, and could give a useful measure of residual impairment of higher sensorimotor function in recovery from general anaesthesia. Saccadic latency and SSRT could also be used as indexes of cortical recovery with a variety of other drugs, when we cannot easily establish the link between drug concentration in the brain and its effect.
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Appendix: a brief summary of the LATER model |
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p'(Hi) = p(Hi).p(E|Hi)/p(E)
where p(Hi) and p'(Hi) are the prior and posterior probabilities, respectively, of the ith competing hypothesis, E is some experimental observation, and p(E|Hi) is the probability of making that observation, given Hi; p(E), the global probability of E, is common to all hypotheses. Taking logarithms:
log(p'(Hi)) = log(p(Hi)) + log(p(E|Hi)) log(p(E))
If information is being presented continuously from a stimulus, then it is clear that log(p(Hi) will rise linearly. There is thus a one-to-one correspondence between this formulation of an ideal decision maker and the LATER model: S0 represents log(p), r represents the rate of arrival of information, and ST represents a criterion level at which the hypothesis is sufficiently supported for a response to be justified: it is equivalent to a significance level in conventional statistics.
Experiments have tested whether this interpretation of the LATER model is correct, by seeing whether, when expectation or the supply of information or the criterion level are changed, the observed effects on the latency distributions are as predicted. So far these tests have proved satisfactory. Other experiments have studied situations where more than one LATER units operate in parallel, embodying the competition that results in one hypothesis being selected rather than another. These too appear to support the LATER model. Finally, neural correlates of such a mechanism have been identified in the frontal cortical eye fields of macaque monkeys through single-unit recording techniques.7 31 Recording from these movement cells, which increase their firing rate just before saccade generation, shows a linear rise in activity whose rate is correlated with latency, whereas the threshold of firing corresponding to saccadic activation remains essentially constant. Thus these neurones show similar behaviour to that predicted by the LATER model: the determination of saccadic latency appears to involve cortical centres that act indirectly on the more primitive brainstem circuitry. This converts the command make the movement into an appropriate pattern of motor nerve activity that will move the eye rapidly and accurately on to its target (and which also, incidentally, determines PSV). Since manual responses as well as saccades appear to follow a recinormal distribution, and for other stimuli (auditory, tactile) as well as visual, there seems to be good grounds for supposing that something like the LATER model represents a fundamental neural process underlying decision making in the brain.
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