Real-time assessment of alpha -ketoglutarate effect on organic anion secretion in perfused rabbit proximal tubules

Apichai Shuprisha, Ronald M. Lynch, Stephen H. Wright, and William H. Dantzler

Department of Physiology, College of Medicine, University of Arizona, Tucson, Arizona 85724


    ABSTRACT
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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

To determine the quantitative roles of the basolateral and luminal Na+-dicarboxylate (Na-DC) cotransporters in establishing and maintaining the alpha -ketoglutarate (alpha KG) gradient required for renal tubular secretion of organic anions, we measured net steady-state transepithelial secretion of fluorescein (FL) in real time in isolated, perfused S2 segments of rabbit renal proximal tubules. Net "basal" FL secretion in the absence of exogenous alpha KG had a Kt of ~4 µM and a maximal transepithelial secretion rate (Jmax) of ~380 fmol · min-1 · mm-1 (where Kt is the FL concentration that produces one-half the Jmax). It could be almost completely inhibited by basolateral p-aminohippurate (PAH). Selective inhibition of the basolateral Na-DC cotransporter indicated that recycling via this transporter of alpha KG that had been exchanged for FL supports ~25% of the "basal" FL secretion. Physiological alpha KG concentrations of 10 µM in the bath or 50 µM in the perfusate stimulated net secretion of FL by ~30 or ~20%, respectively. These data indicate that the basolateral Na-DC cotransporter supports ~42% of the net FL secretion. The luminal and basolateral effects of physiological concentrations of alpha KG were additive, indicating that the combined function of the luminal and basolateral Na-DC cotransporters can support ~50% of the net FL secretion. This apparently occurs by their establishing and maintaining ~50% of the outwardly directed alpha KG gradient that is responsible for driving basolateral FL/alpha KG exchange. The remaining ~50% would be maintained by metabolic production of alpha KG in the cells.

fluorescein; sodium-dicarboxylate cotransporters; transepithelial transport in real time


    INTRODUCTION
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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

a wide variety of organic anions (or weak organic acids that exist as anions at physiological pH), for which p-aminohippurate (PAH) is a prototype, are secreted by the proximal tubules of mammals and most other vertebrates (12, 13). In the S2 segment of mammalian renal proximal tubules, transepithelial secretion of organic anions (OA) involves transport into the cells against an electrochemical gradient at the basolateral membrane and movement from the cells into the lumen down an electrochemical gradient (12). Transport into the cells at the basolateral membrane is a tertiary active process, the final step of which is the transport of OA into the cells against its electrochemical gradient in exchange for a dicarboxylate (DC) [physiologically, alpha -ketoglutarate (alpha KG)] moving down its electrochemical gradient through an OA/DC exchanger (9, 16). The outwardly directed gradient for alpha KG appears to be maintained through a combination of intracellular metabolism and Na+-coupled secondary active uptake of alpha KG across the basolateral membrane. This basic model, first based on studies with renal basolateral membrane vesicles (BLMV) (9, 16), has now been shown to function in intact renal proximal tubules from mammals and reptiles (1, 2, 17, 24). A transporter that mediates OA/DC exchange has now been cloned from mammalian renal tissue (15, 21).

Most studies that have attempted to determine the role of uptake of exogenous alpha KG via Na+-dicarboxylate (Na-DC) cotransport in this OA secretory process have either involved preloading tubules with abnormally high concentrations of alpha KG to stimulate uptake (2, 19) or have involved nonphysiological buffer solutions and temperature (11). Recently, Welborn et al. (24) used physiological concentrations of alpha KG (~10 µM) (14) in the bathing medium to examine the role of the basolateral Na-DC cotransporter in establishing and maintaining the outwardly directed alpha KG gradient for basolateral uptake of OA [using fluorescein (FL)] in isolated renal tubules. Although the medium was as close to physiological as possible in this study, the tubules were not perfused. Therefore, the degree to which basolateral Na-DC cotransport actually functioned to support net transepithelial secretion was still unclear. Moreover, filtered alpha KG is also reabsorbed by a luminal Na-DC cotransporter, which has been cloned and sequenced (8) and shows a higher capacity than the basolateral Na-DC cotransporter (27). It appeared possible that filtered alpha KG taken up from the lumen by this transporter also could contribute to the outwardly directed alpha KG gradient for the basolateral uptake of OA. Indeed, in a previous study with perfused rabbit tubules, we demonstrated that the addition of alpha KG to the lumen could increase net transepithelial PAH secretion (5). However, this only occurred with abnormally high concentrations of alpha KG in the lumen and with reduced PAH secretion in the absence of bicarbonate (5). Therefore, we were not certain that this process was of physiological significance.

To determine more rigorously the roles of the luminal and basolateral Na-DC cotransporters in establishing and maintaining net transepithelial secretion of OA, we developed a system whereby we could measure the net steady-state transepithelial secretion of FL in isolated, perfused renal tubules in real time. Using S2 segments of rabbit renal proximal tubules, we demonstrated that such secretion of FL was a saturable, inhibitable process that occurred via the classic OA (PAH) transport pathway. We also examined and quantified the roles of the luminal and basolateral Na-DC cotransporters in this transepithelial process under conditions as close to physiological as possible. The results clearly indicate that the basolateral Na-DC cotransporter plays a significant role in recycling alpha KG at that membrane even in the absence of exogenous alpha KG. Although the luminal cotransporter appeared to be markedly less important than the basolateral cotransporter, the data indicate that it, as well as the basolateral Na-DC cotransporter, can contribute to basolateral uptake and net transepithelial secretion of FL under physiological conditions when appropriate levels of exogenous alpha KG are present in both the perfusate and bathing medium.


    METHODS
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INTRODUCTION
METHODS
RESULTS
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REFERENCES

Chemicals. Spectral-grade FL and neutral tetramethylrhodamine dextran (TMRD) (40,000 mol wt) were purchased from Molecular Probes (Eugene, OR). All other chemicals were purchased from commercial sources and were of the highest purity available.

Solutions. A modified rabbit Ringer solution, used throughout the studies (unless otherwise indicated) as dissection buffer, superfusion bathing buffer, and perfusing solution, consisted of the following (in mM): 110 NaCl, 25 NaHCO3, 5 KCl, 2 Na2HPO4, 1.8 CaCl2, 1 MgSO4, 10 sodium acetate, 8.3 D-glucose, 5 L-alanine, 4 lactate, and 0.9 glycine; and was adjusted to pH 7.4 with HCl or NaOH. This solution was gassed continuously with 95% O2-5% CO2 to maintain the pH. The bathing medium also contained 3 g/100 ml neutral dextran (40,000 ± 3,000 mol wt) to approximate the plasma protein concentration. The osmolarity of the solution was ~290 mosmol/kgH2O.

Preparation of isolated tubules. New Zealand White rabbits, purchased from Myrtle's Rabbitry (Thompson Station, TN), were killed by intravenous injection of pentobarbital sodium. The kidneys were flushed via the renal artery with an ice-chilled solution containing 250 mM sucrose and 10 mM HEPES, adjusted to pH 7.4 with Tris base. They were then gently removed and sliced transversely using a single-edge razor. A kidney slice was placed in a petri dish containing ice-chilled dissection buffer aerated with 95% O2-5% CO2. Dissection of tubules from a slice was performed manually from the cortical zone without the aid of enzymatic agents. All dissections were performed at 4°C, but all experiments were performed at 37°C. We used only proximal S2 segments in this study, because the S2 segment of the rabbit proximal tubule is the primary site of OA (e.g., PAH) secretion (25).

Perfusion of tubules. The in vitro perfusion technique used in these studies was the same as that described previously (3, 4) with some modification so that the collecting pipette had a length of uniform diameter that could be positioned parallel to the bottom of the bathing chamber to serve as a flow-through cuvette (Fig. 1). The outside diameter of the collecting pipette was ~120 µm, and the inside diameter was ~100 µm. The design of the collecting pipette reduced background fluorescence from the bath, which was caused by the addition of FL during transepithelial secretion studies, sufficiently to permit a simple correction. Each isolated tubule was transferred into a custom-made, temperature-controlled chamber with a coverslip as the bottom. Both tubule ends were held in glass micropipettes, and the tubule was perfused through a micropipette with its tip centered in the tubule lumen at a rate of ~10-15 nl/min. The chamber was continuously superfused with bathing medium at ~3 ml/min and the temperature of the incoming solution was controlled at 37°C as described previously (24). During perfusion experiments, FL was added to the superfusion bathing media and TMRD was added to the perfusion solution as a volume marker.


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Fig. 1.   Instrumentation for real-time measurement of transepithelial secretion of fluorescein (FL). TMRD, tetramethylrhodamine dextran. See METHODS for details.

Measurement of FL and TMRD in collected perfusate. Figure 1 shows the instrument setup diagrammatically. The perfusion chamber was mounted on the stage of an inverted microscope (Olympus model IMT-2) fitted with epifluorescence optics. A ×60 oil-immersion objective (1.4 numerical aperture, Olympus) was used to focus excitation light from a 100-W mercury arc lamp and to collect fluorescence emitted from the solution in the collecting pipette. The intensity of excitation light was reduced by a 2.0 neutral density filter (Oreil, Stratford, CT). Both FL and TMRD were excited at 490 ± 10 nm using a selective band-pass filter (Oriel) for this wavelength. The excitation light was reflected to the sample with a 490DRLP dichroic filter (Omega Optical, Brattleboro, VT), which passed more than 90% of emitted light above 505 nm. The emission fluorescence was first limited to an area of 50 µm diameter by an iris diaphragm and then separated into two beams by a second dichroic mirror (540DRLP, Omega Optical). Each beam was appropriately filtered (520 ± 10 nm for FL; 580 ± 30 nm for TMRD; Oriel and Omega Optical, respectively), and the two beams were simultaneously counted, each by a separate photomultiplier tube (model HC120; Hamamatsu, Bridgewater, NJ) in photon-counting mode. The fluorescence intensity was integrated at 1-s intervals and saved for subsequent analysis with a MSC II data acquisition microcomputer interface and software purchased from Oxford Instrument (Oak Ridge, TN). Figure 2A shows a fluorescence profile of FL and TMRD during transepithelial secretion (in arbitrary units) by an S2 segment of proximal tubule after the bathing medium was changed to one containing 250 nM FL as indicated. At the beginning of the experiment (Fig. 2A), 30 mg/100 ml TMRD was added to the perfusion solution as a volume marker without any interference with the signal in the FL-detecting channel. During transepithelial secretion of FL, however, fluorescence from FL interfered with fluorescence from TMRD in the TMRD-detecting channel. The amount of this interference of FL in the TMRD-detecting channel was determined from standard curves (see below) and simply subtracted to obtain the actual counts for TMRD in the collected perfusate.


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Fig. 2.   A: fluorescence profiles for FL and TMRD recorded simultaneously from the center of a pipette collecting fluid from an isolated perfused S2 segment of rabbit proximal tubules during a transepithelial secretion study. At the beginning of the experiment, TMRD (30 mg/100 ml) was added to the perfusion solution as a volume marker. This resulted in fluorescence in the TMRD channel without any detectable counts in the FL channel. When the bathing medium was switched to one containing 250 nM FL as well as TMRD, there was a small and rapid increase in background fluorescence within 30 s (not clearly seen in the tracings) followed by an increase in fluorescence from FL that was secreted into the perfused lumen. FL fluorescence was detected in both channels. The larger signal for FL in the TMRD channel than in the FL channel reflects the higher gain in the TMRD channel required to detect the TMRD signal. However, a correction for the contribution of FL fluorescence to the TMRD signal can be made (see methods), thereby providing the actual counts of TMRD in collected perfusate. B and C: actual concentrations of FL (B) and TMRD (C) in collected perfusate. These were obtained after the original counts in the collected perfusate from each channel were corrected and converted using standard curves constructed at the end of the experiment (all settings and the position of the collection pipette during generation of the standard curves were the same as those during the experiment) (see methods).

Determination of FL and TMRD concentrations in collected perfusate. Concentrations of FL and TMRD were determined from standard curves constructed at the end of each experiment by retrograde infusion of known concentrations of FL or TMRD into the collecting pipette while the bathing solution contained either only bathing solution or bathing solution plus the appropriate concentration of FL. The background fluorescence of FL in the bathing medium during transport studies was determined by infusing perfusion solution alone into the collecting pipette while the bathing solution contained the appropriate concentration of FL for that experiment. The background reading usually averaged less than 1% of total fluorescence during secretion studies. To construct standard curves, we averaged twenty 1-s data points for each FL or TMRD concentration. The interference of the FL fluorescence in the TMRD-detecting channel was also determined during infusion of FL into the collecting pipette. The autofluorescence and the appropriate FL background counts were subtracted from the counts obtained during net secretion to yield the actual FL or TMRD counts in the collecting pipette. The photon count was then converted into concentration from the standard curves. Figure 2, B and C, shows profiles of the FL and TMRD signals from Fig. 2A after conversion to concentrations. About 30 s after adding 250 nM FL to the bathing medium, fluorescence began to rise and reached a steady state within about 5 min. The concentration of FL in the collected perfusate was ~16 times higher than the concentration in the bath indicating transepithelial transport against a concentration gradient. The concentration of TMRD did not change much in this and subsequent studies. Therefore, no measurements of volume change due to water reabsorption were made, and TMRD was used simply as an indicator of leaks in the tubule throughout the study.

Measurement of transepithelial secretion of FL. The net transepithelial secretion of FL, JFL (in mol · min-1 · mm-1), was determined from the following relationship
<IT>J</IT><SUB>FL</SUB> = <FR><NU>V<SUB>C</SUB>C<SUB>C</SUB></NU><DE><IT>L</IT></DE></FR>
In the equation, VC is the perfusion rate (in nl/min) measured directly; CC is the concentration of FL (in mol/nl) in the collected perfusate in the collecting pipette during steady-state net secretion; and L is the length of the perfused tubule (in mm) measured with an ocular micrometer. This equation is based on the assumption that there is essentially no backflux of FL from lumen to bath, an assumption shown to hold for PAH (4, 22). The perfusion rate was ~10-15 nl/min. Following the addition of FL to the bath, its concentration at steady state was determined by a 1-min average of data points.

Statistical analysis. Results are summarized as means ± SE; n is the number of experiments. One tubule from one animal was used for each experiment. Replicates for each experiment in a single tubule were averaged to represent a single value for that experiment. Differences in steady-state transepithelial secretion rates were evaluated by either a paired t-test, a one-way, two-sample t-test, or an ANOVA followed by a multiple contrast posttest employing the Dunnett method as indicated in the legends to Figs. 1-9. Differences were assumed to be significant when P < 0.05.


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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

Kinetics of steady-state transepithelial secretion by isolated perfused S2 segment of renal proximal tubules. Initially, we examined the profile of steady-state transepithelial secretion of FL and determined kinetic parameters of the transport process by S2 segment of proximal tubules. Figure 3 represents a profile of transepithelial secretion by an isolated perfused S2 segment that was incubated in a continuously flowing bathing medium that was alternately switched from a FL-free medium to one containing FL at concentrations ranging from 250 nM to 10 µM. For each concentration of FL, a steady-state secretion of FL was reached ~5 min after the FL was added. This steady-state secretion of FL increased rapidly as the FL concentration increased from 0.25 to 2 µM and then leveled off at higher concentrations (Fig. 3), a typical characteristic of a saturating transport process. Table 1 summarizes the transport rate of FL at different concentrations by isolated perfused S2 segments of proximal tubules in which the perfusion rate was held constant for each tubule but varied from 10-15 nl/min among this set of experiments. Also shown in this table is the tubular fluid-to-bath ratio (TF/B) of 1-mm tubule length (n = 10). This ratio is greater than unity at all concentrations of FL added to the bath, indicating that transepithelial secretion involves transport against a concentration gradient. This ratio decreased gradually from ~5 to ~1 as the concentration of FL increased from 0.25 to 10 µM, suggesting that the transepithelial secretion involved a carrier-mediated process in which carriers were becoming saturated at high concentrations of substrate. Indeed, four of the tubules showed TF/B values of less than 1.0 with 10 µM FL in the bath, indicating that the secretory process was already saturated in these tubules. The kinetic profile of transepithelial secretion of FL by the S2 segment of proximal tubules can be adequately described by the following equation
<IT>J</IT><SUB>FL</SUB> = <FR><NU><IT>J</IT><SUB>max</SUB>[FL]<SUB>bath</SUB></NU><DE><IT>K</IT><SUB>t</SUB> + [FL]<SUB>bath</SUB></DE></FR> (1)
This equation has the same form as the Michaelis-Menten relationship, where JFL is net transepithelial secretion rate at steady state, Jmax is the maximal transepithelial secretion rate, and Kt is the FL concentration at one-half Jmax. Kinetic analysis revealed a Kt of ~4 µM and a Jmax of ~280 fmol · min-1 · mm-1 (Fig. 3B). Subsequently, we used FL as the OA substrate at a concentration of 1 µM, which is well below the Kt, throughout the rest of our studies.



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Fig. 3.   A: a sample tracing of FL transepithelial secretion rate profile in fmol · min-1 · mm-1 from an isolated perfused S2 segment of rabbit proximal tubules in response to addition of various concentrations of FL to the bath. Tracing was obtained after conversion of concentration profile (as in Fig. 2) using perfusion rate and length of the tubule measured directly in the experiment. A steady-state transepithelial secretion was reached (usually in ~5 min) after FL was added. Then the bathing medium was switched back to standard buffer containing no FL (wash). B: effect of increasing FL concentration (shown in µM on abscissa) on rate of transepithelial secretion by perfused S2 segments of rabbit renal proximal tubules (shown in fmol · min-1 · mm-1 on ordinate). Each point represents mean ± SE (n = 10). Kinetic parameters, Kt and Jmax, were derived for each tubule with a nonlinear regression algorithm (Enzfitter, Biosoft). Line fitted to data was calculated from Eq. 1 using averaged kinetic parameters obtained from each tubule.


                              
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Table 1.   Steady-state tubular fluid-to-bath ratio and transepithelial secretion rate of FL at different FL concentrations

Inhibition of transepithelial secretion of FL by PAH. In recent years, FL has been used as a model substrate for the peritubular OA/DC exchanger on the assumption that the basolateral uptake of FL utilizes the classic OA (PAH) transporter. Increasing evidence supports the idea that basolateral uptake of FL by proximal tubules involves and is limited to the PAH transporter (19, 23, 24). However, we wished to establish that transepithelial secretion of FL involves the same transport system as that for PAH. That this is the case is indicated by the data shown in Fig. 4. The transport rate of FL was decreased when PAH was added during the steady-state secretion of 1 µM FL. The inhibition increased with increasing PAH concentration and was completely reversed after removal of PAH. The kinetic profile suggested competitive inhibition by PAH. The kinetic parameters were calculated by a modification of the isotope dilution procedure of Malo and Berteloot (7) according to the following equation
<IT>J</IT> = <FR><NU><IT>J</IT><SUB>max</SUB>[*T]</NU><DE><IT>K</IT><SUB>t</SUB> <FENCE><FR><NU>1 + [I]</NU><DE><IT>K</IT><SUB>i</SUB> + [*T]</DE></FR></FENCE></DE></FR> + D[*T] (2)
where J, Jmax, and Kt are as previously defined, [I] is the concentration of inhibitor (PAH), Ki is the Michaelis constant of the inhibitor, [*T] is the concentration of FL, and D is a coefficient describing nonsaturable transport (passive diffusion). Equation 2 can be rearranged to give the following
<IT>J</IT> = <FR><NU><IT>J</IT><SUB>app</SUB>[*T]</NU><DE><IT>K</IT><SUB>i app</SUB> + [I]</DE></FR> + C (3)
where Japp is defined as (Ki/Kt)Jmax, Ki app is an apparent Ki that is defined as Ki(1 + [*T]/Kt), and C is a constant derived from D and [*T] reflecting passive transepithelial flux through or between tubule cells. Therefore, knowledge of J, [I], and [*T] permits the calculation of Japp, Ki app, and C using nonlinear regression analysis. When [*T] is << Kt, Ki app approx  Ki. Analysis of 12 separate experiments yielded a Ki app value for the inhibition of FL secretion by PAH of ~108 µM (Fig. 4B). This value is almost identical to the concentration for half-maximum transport (Kt) concentration of PAH of ~110 µM reported previously for S2 segments of rabbit proximal tubules (6, 17). The similarity between these kinetic parameters strongly supports the idea that FL and PAH share the same transport system. In addition, transepithelial secretion of 1 µM FL decreased from 45 ± 6 to 4 ± 1 fmol · min-1 · mm-1 (91% inhibition) (Fig. 4B), when 5 mM PAH was added to the bathing medium. This finding further indicates that transepithelial secretion of FL is essentially limited to the PAH transport system. In other words, it indicates that less than 10% of transepithelial secretion of FL is by some other pathways (e.g., passive paracellular flux).



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Fig. 4.   A: a sample tracing of FL transepithelial secretion rate profile in fmol · min-1 · mm-1 from an isolated perfused S2 segment of rabbit proximal tubules in response to addition of various concentrations of p-aminohippurate (PAH) to bath. FL concentration was 1 µM. Addition of PAH to bath during steady secretion of FL resulted in a concentration-dependent reversible inhibition profile. B: effect of increasing PAH concentration (shown in mM on abscissa) on steady-state transepithelial secretion rate of 1 µM FL by perfused S2 segments of rabbit renal proximal tubules (shown in fmol · min-1 · mm-1 on ordinate). Each point represents mean ± SE (n =12, except at PAH concentrations of 0.02 and 0.05 mM, where n = 11). Kinetic parameters, Ki app and Japp, were derived for each tubule with a nonlinear regression algorithm (Enzfitter, Biosoft). Line fitted to data was calculated by a modification of isotope dilution procedure of Malo and Berteloot (7) using averaged kinetic parameters obtained from each tubule.

Influence of basolateral Na-DC cotransporter on transepithelial secretion of FL in the absence of exogenous alpha KG. The basolateral Na-DC cotransporter plays an important role in the tertiary transport model for peritubular uptake of OA by helping to maintain the in > out gradient for alpha KG that otherwise could be dissipated during exchange for peritubular OAs (9, 13, 16). However, the actual extent to which recycling of alpha KG by the basolateral Na-DC cotransporter contributes to the transepithelial secretion of OAs is not certain. To obtain a quantitative assessment of the importance of reuptake by the basolateral Na-DC cotransporter of alpha KG that has been exchanged for FL, we inhibited this cotransporter with LiCl in the absence of exogenous alpha KG. We added 2 mM LiCl to the bathing medium in the absence of exogenous alpha KG while measuring the transepithelial secretion of 1 µM FL. This concentration of LiCl has been shown to inhibit the Na-DC cotransporter (26). When LiCl was added to the bathing medium, FL secretion decreased from the control value of ~54 to 41 fmol · min-1 · mm-1, a decrease of ~23% (Fig. 5). This inhibition was reversible upon removal of LiCl from the bathing medium. In contrast, when the same concentration of LiCl was added to the luminal perfusion solution, it had no effect on FL secretion (Fig. 5). Taken together, these results indicate that the inhibitory effect of LiCl when added to the bath is likely limited to inhibition of alpha KG recycling by the basolateral Na-DC cotransporter rather than to other metabolic effects of any LiCl that might have entered the cells via the Na-DC cotransporters located on either basolateral or luminal side. Therefore, we conclude that recycling of alpha KG by the basolateral Na-DC cotransporter contributes ~25% to net OA secretion.


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Fig. 5.   Effect of 2 mM LiCl added to bath or perfusion solution (lumen) on steady-state transepithelial secretion of 1 µM FL by isolated perfused S2 segments of rabbit renal proximal tubules. Values are means ± SE. Numbers in parentheses indicate numbers of experiments. * Significantly different from control group (P < 0.05) as determined by ANOVA.

Influence of exogenous basolateral alpha KG on transepithelial secretion of FL. Most previous studies on mammalian proximal tubules used nonphysiologically high concentrations (100 µM) of dicarboxylates, alpha KG or glutarate, for preloading the cells to maximize the stimulatory effect on OA transport in a dicarboxylate-free medium (2, 20). This protocol does not take into consideration the effect of the physiologically available alpha KG in the plasma, ~10 µM (14), on OA transport. Although Pritchard (11) showed that the addition of 10 µM alpha KG to the medium bathing rat renal cortical slices caused a 30% increase in PAH uptake, the experiments were performed under nonphysiological conditions (i.e., at room temperature, and in nutrient-free and bicarbonate-free phosphate buffer), conditions that are likely to compromise cellular metabolism and rates of OA transport. Therefore, we sought to evaluate the extent to which exogenous alpha KG influences FL transport under conditions resembling as closely as possible those to which proximal tubules are exposed in vivo (i.e., nutrient-rich bicarbonate buffer at 37°C).

We first determined the influence of exogenous peritubular alpha KG on steady-state transepithelial secretion of 1 µM FL by adding increasing concentrations of alpha KG to the bathing medium. Figure 6 is a typical profile of transepithelial secretion of 1 µM FL by an isolated perfused S2 segment of proximal tubules in response to exogenous peritubular alpha KG at concentrations ranging from 5 µM to 1 mM. As shown in Fig. 6 and summarized in Table 2, the addition of alpha KG to the bathing medium affected FL secretion in a biphasic manner. Concentrations of alpha KG below 200 µM significantly stimulated FL secretion; concentrations of 500 µM and above significantly inhibited it in a concentration-dependent manner. These findings support the idea that high concentrations of dicarboxylates interact competitively with OAs at the extracellular face of the OA/DC exchanger. Maximum stimulation of secretion (~40-50%) was found at alpha KG concentrations ranging from 10 to 100 µM. Upon removal of 1 mM alpha KG from the bathing medium, there was an abrupt increase in FL secretion above the control level, presumably due to the accumulation of alpha KG within the cells following the incubation at high concentrations of alpha KG (i.e., the equivalent of preloading renal tubules with a high concentration of alpha KG). This increase was transient and gradually decreased to the control level in the absence of exogenous alpha KG (Fig. 6). The finding that 10 µM of peritubular alpha KG stimulated net secretion of FL ~40% (Table 2) indicates that under physiological conditions uptake of exogenous alpha KG by the basolateral Na-DC cotransporter can support ~29% of net OA secretion; i.e., [(increase in secretion in presence of 10 µM alpha KG in bath from the "basal" state)/(net secretion in presence of 10 µM alpha KG in bath)] × 100%. Although it appeared most likely that alpha KG that entered the tubule cells via the basolateral Na-DC cotransporter stimulated transepithelial FL secretion by countertransport at the basolateral membrane, it was also possible that stimulation could have resulted from metabolism of alpha KG. To be certain that this was not the case, in another set of experiments, we examined the effects of glutarate or alpha KG in the bath on FL secretion by the same tubule. Glutarate is not significantly metabolized by the renal cells (10) and is one of the few dicarboxylates other than alpha KG that is exchanged for PAH at the basolateral membrane (9). As shown in Fig. 7, the addition of 10 µM glutarate to the bath stimulated transepithelial secretion of FL to an extent similar to that of 10 µM alpha KG (~37% and ~24% of control, respectively). There was no significant difference between the stimulation produced by glutarate and alpha KG. In addition, 1.0 mM glutarate, like 1.0 mM alpha KG, inhibited the secretion (~57% and ~51% of control, respectively). Therefore, alpha KG that enters the renal tubule cells via the basolateral Na-DC cotransporter apparently stimulates net FL secretion by exchange for FL at the basolateral membrane.


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Fig. 6.   A representative tracing showing a biphasic effect of alpha -ketoglutarate (alpha KG) in bath on transepithelial secretion rate of FL in fmol · min-1 · mm-1 by an isolated perfused S2 segment of rabbit proximal tubules. FL concentration was 1 µM. Upon removal of 1 mM alpha KG from the bathing medium, there was an abrupt increase in FL secretion above the control level, presumably due to the accumulation of alpha KG following the incubation at a high concentration of alpha KG; i.e., effect of preloading renal tubules with a high concentration of alpha KG. This increase was transient and gradually decreased to the control level in absence of exogenous alpha KG.


                              
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Table 2.   Effect of basolateral alpha KG on steady-state transepithelial secretion of 1 µM FL



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Fig. 7.   Effect of alpha KG or glutarate added to bath on transepithelial secretion of 1 µM FL by isolated perfused S2 segments of rabbit proximal tubules. Concentrations of alpha KG or glutarate are indicated above each pair of bars. Preload group represents the result obtained after removal of 1 mM alpha KG or glutarate from the bath as described in Fig. 6. Values are means ± SE (n = 7). No difference was observed between effects of alpha KG and glutarate. a Significantly different from control group (P < 0.05). b Significantly different from other experimental groups (P < 0.05). Differences were determined by ANOVA.

Influence of exogenous luminal alpha KG on transepithelial secretion of FL. In rabbit renal tissue, the luminal Na-DC cotransporter, which has been cloned and sequenced (8), shows a higher capacity than the basolateral Na-DC cotransport. Because alpha KG is also filtered, it can be readily reabsorbed from the lumen by the luminal Na-DC cotransporter. Therefore, we hypothesized that the luminal uptake of filtered alpha KG could be as important as, or perhaps more important than, the uptake of alpha KG across the basolateral membrane in establishing the in > out alpha KG gradient for basolateral OA/DC exchange. To evaluate this possibility, we explored the effects of alpha KG in the lumen on net FL secretion by isolated, perfused S2 segments of proximal tubules in the absence of alpha KG in the bathing medium. As shown in Fig. 8, addition of alpha KG to the perfusion solution stimulated steady-state transepithelial secretion of 1 µM FL at every concentration tested. However, at the presumed physiological luminal alpha KG concentrations of ~25 to 50 µM, FL secretion was stimulated by only ~15-20%. From these results, we suggest that luminal uptake of alpha KG via the luminal Na-DC cotransporter can contribute to transepithelial secretion of OAs by ~15%; i.e., [(the increase in secretion in the presence of 50 µM alpha KG in the lumen from the "basal" state)/(net secretion in the presence of 50 µM alpha KG in the lumen)] × 100%. The data also indicate that at physiological exogenous alpha KG concentrations, the basolateral Na-DC cotransporter is more effective than the luminal Na-DC cotransporter in promoting OA secretion.


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Fig. 8.   Effect of alpha KG added to perfusion solution on transepithelial secretion of 1 µM FL by isolated perfused S2 segments of rabbit proximal tubules. Values are means ± SE. Numbers in parentheses indicate numbers of experiments. a Significantly different from control group (P < 0.05). b Significantly different from groups at a concentration of 0.2 mM and below (P < 0.05). Differences were determined by one-way, paired t-test.

Influence of both basolateral and luminal exogenous alpha KG on transepithelial secretion of FL. Under normal in vivo conditions, the renal tubule cells are always exposed to dicarboxylates on both basolateral and luminal sides. Transepithelial secretion of OAs, therefore, occurs under conditions where exchangeable dicarboxylates are distributed at steady state within the cells by metabolism and continuous uptake from both sides of the tubule cells. To evaluate the contribution of both basolateral and luminal Na-DC cotransporters to transepithelial secretion of OAs, we examined the effects of alpha KG in the lumen, in the bath, and in both lumen and bath simultaneously on steady-state transepithelial secretion of 1 µM FL by the same individual S2 segment of proximal tubules. The concentrations of alpha KG added were 10 µM to the bath and 50 µM to the perfusion solution, on the assumption that the latter concentration could possibly be in tubular fluid reaching the S2 segment in vivo. The results are shown in Fig. 9. Addition of alpha KG to either the luminal or the basolateral side produced an increase in transepithelial secretion of 1 µM FL by ~13 or 15%, respectively. Interestingly, when the tubule was exposed on both sides to these same concentrations of alpha KG, the secretion increased to 28%. These data indicate that alpha KG that enters the cells via the luminal Na-DC cotransporter can further support transepithelial secretion of OAs by increasing the cellular pool of exchangeable alpha KG at the basolateral membrane.


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Fig. 9.   Effect of alpha KG added to bath, perfusion solution (lumen), or both on the transepithelial secretion of 1 µM FL by isolated perfused S2 segments of rabbit renal proximal tubules. Values are means ± SE (n = 9). * Significantly different from control group (P < 0.05). ** Significantly different from other experimental groups in which alpha KG was added either to bath or perfusion solution (P < 0.05). Differences were determined by ANOVA.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

In the present study, we evaluated the transepithelial secretion of the organic anion FL by isolated, perfused S2 segments of rabbit renal proximal tubules in real time by using a specially constructed epifluorescence system in which the collecting pipette functioned as a flow-through cuvette. The studies were also performed under conditions that were as close to physiological as possible, i.e., nutrient-enriched, bicarbonate-buffered bathing and perfusing solutions at 37°C. Initially, we demonstrated that the steady-state transepithelial secretion of FL saturated with an apparent Kt of ~4 µM and Jmax of ~280 fmol · min-1 · mm-1. These values were similar to those reported by Sullivan et al. (19) for the basolateral uptake of FL by nonperfused S2 segments of rabbit renal proximal tubules (Kt = 10 µM; Jmax = ~ 498 fmol · min-1 · mm-1). Although these authors had doubts about the reliability of the value for Jmax (19), the similarity between the values for perfused tubules in the current study and those for nonperfused tubules in the earlier study (19) strongly suggests that basolateral transport into the cells is the rate-limiting step for transepithelial transport. Our initial studies also confirmed the assumption that FL transport occurs by the classic OA (PAH) pathway, for PAH inhibited the transepithelial FL transport with an apparent Ki almost identical to the Kt reported previously for PAH transport at the basolateral membrane of this same rabbit tubule segment (6, 17). In the present study, we used the parallel measurements of fluorescence from TMRD in the lumen only to check for leaks during the perfusion. However, with higher concentrations of TMRD in the perfusate, it would be possible to make online measurements of volume change resulting from tubular reabsorption of perfusate.

The use of epifluorescence microscopy to study transepithelial secretion of FL in perfused tubules in real time also allowed us to investigate directly the extent to which the activity of the Na-DC cotransporters located on both the basolateral and luminal membranes contributed to the net transepithelial secretion of OAs. The tertiary active transport model for OA transport at the basolateral membrane suggests that alpha KG is recycled through the parallel activity of the OA/DC exchanger and the basolateral Na-DC cotransporter (12). The recent study by Welborn et al. (24) on nonperfused rabbit S2 renal proximal tubules indicates that reuptake by the basolateral Na-DC cotransporter of alpha KG that has moved out of the cells in exchange for FL accounts for ~25% of the initial rate of basolateral FL uptake in the absence of exogenous alpha KG. Similarly, in the current study on perfused tubules, inhibition of the basolateral Na-DC cotransporter indicated that reuptake of alpha KG by this transporter accounts for ~25% of the steady-state transepithelial secretion of FL in the absence of exogenous alpha KG. Therefore, the parallel activities of the basolateral OA/DC exchanger and the basolateral Na-DC cotransporter recycling alpha KG apparently account for the maintenance of ~25% of the outwardly directed gradient for alpha KG and the corresponding basolateral uptake and transepithelial secretion of OA in the absence of exogenous alpha KG.

Under physiological conditions in vivo, renal tubules are exposed to ~10 µM alpha KG from the blood (bathing medium) side. In previous work on nonperfused rabbit S2 renal proximal tubules, the addition of this concentration of alpha KG to the bathing medium (which was identical to that used in the present study) containing 1 µM FL led to an increase of ~75% in the initial rate of FL uptake (24). In the present study with perfused tubules, the addition of 10 µM alpha KG to the bathing medium containing 1 µM FL led to an increase of ~15% to ~40% in the steady-state transepithelial secretion of FL (Table 2; Figs. 7 and 9). The difference in degree of stimulation between our current study on perfused tubules and the previous one on nonperfused tubules (24) may reflect differences in: 1) transport measured (steady-state transepithelial secretion vs. initial rate of basolateral uptake); 2) intracellular distribution of alpha KG taken up from the bath because of differences in the metabolic state of perfused vs. nonperfused tubules; and 3) the exchangeable intracellular pool of alpha KG in tubules from different rabbits. The variability between tubules from different rabbits was particularly marked in the current study with perfused tubules (compare data in Table 2 and Figs. 7 and 9). These differences may indeed reflect differences in metabolic state and the available exchangeable intracellular pool of alpha KG produced by metabolism. This possibility is lent some credence by the observation that glutarate, which is not significantly metabolized (10), tended to produce a slightly higher stimulation of FL transport at 10 µM and inhibition of FL transport at 1 mM than alpha KG at the same concentrations (Fig. 7).

In the present study on perfused S2 segments of rabbit proximal tubules, it was possible to evaluate the contribution of alpha KG transport into the cells by the luminal Na-DC cotransporter to the transepithelial secretion of FL in real time. We found that the addition of a concentration of alpha KG to the lumen that might be expected to be present physiologically (assuming that normal fluid absorption concentrates the filtered alpha KG before it reaches the S2 segment) produced a significant increase in steady-state net secretion of FL in the absence of alpha KG in the bathing medium. This stimulation was demonstrated in the presence of bicarbonate-buffered perfusing and bathing solutions, which are essential to maintaining physiological levels of tricarboxylic acid cycle intermediates, such as alpha KG, in the cells and, thus, normal levels of OA transport (18). In a previous study on perfused S2 segments, Dantzler and Evans (5) also showed that the addition of alpha KG or glutarate to the lumen could stimulate net transepithelial secretion of radiolabeled PAH in the absence of alpha KG in the bathing medium. This stimulation was prevented if the luminal Na-DC cotransporter was inhibited by the simultaneous inclusion of LiCl in the perfusate with the alpha KG or glutarate. However, in this previous study, the stimulation was apparent only with very high concentrations of alpha KG or glutarate in the lumen and only when the control rate of net steady-state PAH secretion was depressed by using bicarbonate-free perfusate (5). In retrospect, it appears that relatively high concentrations of malate and citrate in the bicarbonate-buffered solutions may have prevented alpha KG uptake from the lumen by competing for the luminal Na-DC cotransporter. Indeed, we found during the present study that when the bathing solution contained malate and citrate in the presence of a 10 µM concentration of alpha KG, the usual stimulatory effect on FL secretion was reduced, presumably by competition between malate, citrate, and alpha KG for the basolateral Na-DC cotransporter (unpublished observations). Moreover, the simple removal of malate and citrate from the bathing medium produced an increase in FL secretion (unpublished observations). In this case, malate and citrate were probably inhibiting reuptake of alpha KG by the basolateral Na-DC cotransporter.

Although kinetic data are not available for the transport of alpha KG itself by either the luminal or basolateral Na-DC cotransporter, studies of succinate transport by brush-border membrane vesicles (BBMV) and BLMV provide some information on the possible affinities and capacities of these two transporters for alpha KG (27). In BBMV, the Kt and Jmax for succinate are ~600 µM and ~90 nmol · min-1 · mm-1, respectively; in BLMV, they are ~10 µM and ~5 nmol · min-1 · mm-1 (16). The inhibitory effects of alpha KG on succinate transport suggest that the Kt values for it may be similar to those for succinate (27). These data suggest that physiological concentrations of alpha KG in the lumen should certainly be taken up by the luminal Na-DC cotransporter to contribute to the gradient for FL/alpha KG exchange at the basolateral membrane, as clearly occurred in the present study. However, in general, 50 µM alpha KG in the lumen had less stimulatory effect on transepithelial FL secretion (in the absence of alpha KG in the bath) than did 10 µM alpha KG in the bath (in the absence of alpha KG in the lumen). This observation might be related to the anatomic proximity of the basolateral Na-DC cotransporter to the basolateral OA/DC exchanger. The alpha KG transported into the cells via the basolateral Na-DC cotransporter might be supplied relatively directly to the OA/DC exchanger, whereas the alpha KG transported into the cells by the luminal Na-DC cotransporter might contribute to both the intracellular pool of exchangeable alpha KG for the OA/DC exchanger and to other cellular metabolic events.

In the previous study on the role of the luminal uptake of alpha KG in the process of PAH secretion, the time course of the radioisotopic measurements markedly limited the sensitivity of the determinations of PAH secretion, and it was not feasible to study the effects of alpha KG in the lumen and bathing medium simultaneously (5). In the present study, it was possible to determine the effect of alpha KG in the lumen alone, the bath alone, and in both the lumen and bath simultaneously on steady-state transepithelial FL secretion in the same individual tubules. The data revealed a significant additive effect on FL secretion when alpha KG was in both the lumen and the bath at approximately physiological concentrations (Fig. 9). Therefore, the luminal uptake of alpha KG can play a role in maintaining secretion of OA under physiological conditions with alpha KG present at the basolateral side. However, it should be noted that in the series of experiments reported in Fig. 9, the degree of stimulation of FL secretion by 10 µM alpha KG in the bathing medium was substantially less than that in other experiments (for example, Table 2). Therefore, although uptake of alpha KG from the lumen can contribute to OA secretion even with alpha KG in the bath, the contribution may be less when the basolateral uptake is more effective.

The present observations on the effects of alpha KG permit us to estimate the extent to which the basolateral and luminal Na-DC cotransporters may contribute to net transepithelial secretion of OAs under normal physiological conditions. Recycling of alpha KG by the basolateral Na-DC cotransporter supports ~25% of the "basal" secretion in the absence of exogenous alpha KG. The basolateral Na-DC cotransporter can also support a further ~30% (average value from all experiments) of OA secretion by uptake of exogenous alpha KG from the physiological basolateral concentration of 10 µM. Therefore, the activity of the basolateral Na-DC cotransporter is responsible for supporting ~42% of the transepithelial secretion of OAs; i.e., {[(increase in secretion in presence of 10 µM alpha KG in bath from the "basal" state) + (difference in secretion in presence of 2 mM LiCl in bath from the "basal" secretion)]/(net secretion in presence of 10 µM alpha KG in bath)} × 100%. The luminal Na-DC cotransporter can support an additional ~20% (average value from all experiments) of OA secretion by uptake of alpha KG from the luminal fluid, if the luminal concentration is 50 µM. As noted above, the effect of alpha KG uptake by both the luminal and basolateral Na-DC cotransporters on OA secretion can be additive with physiological concentrations of alpha KG in lumen and bath. If this is the case and if we use the average individual values obtained, we can conclude that the luminal and basolateral Na-DC cotransporters together directly support ~50% of the net transepithelial OA secretion; i.e., {[(increase in secretion in presence of 10 µM alpha KG in bath from the "basal" state) + (difference in secretion in presence of 2 mM LiCl in bath from the "basal" secretion) + (increase in secretion in presence of 50 µM alpha KG in lumen from the "basal" state)]/(net secretion in presence of 10 µM alpha KG in bath and 50 µM alpha KG in lumen)} × 100%. We assume that this occurs by these transporters establishing and maintaining ~50% of the outwardly directed gradient for alpha KG that is responsible for driving the OA/DC exchange at the basolateral membrane. The remaining ~50% would be maintained by metabolic production of alpha KG in the cells.


    ACKNOWLEDGEMENTS

We thank the Royal Thai Government for support of A. Shuprisha during the course of this work.


    FOOTNOTES

This study was supported in part by National Institutes of Health Research Grant ES-06757, by Training Grants HL-07249, NS-07309, and GM-08400, and by Southwest Environmental Health Science Center Grant ES-06694.

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

Address for reprint requests and other correspondence: W. H. Dantzler, Dept. of Physiology, College of Medicine, Univ. of Arizona, Tucson, AZ 85724-5051 (E-mail: dantzler{at}u.arizona.edu).

Received 15 March 1999; accepted in final form 21 May 1999.


    REFERENCES
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

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6.   Dantzler, W. H., K. K. Evans, and S. H. Wright. Kinetics of interactions of para-aminohippurate, probenecid, cysteine conjugates and N-acetyl cysteine conjugates with basolateral organic anion transporter in isolated rabbit proximal renal tubules. J. Pharmacol. Exp. Ther. 272: 663-672, 1995[Abstract].

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