Departments of 1Cell Biology and 4Medical Biochemistry, University of Aarhus, DK-8000 Aarhus C, Denmark; 2Department of Biochemistry, Faculty of Pharmacy, Wroclaw Medical University, 50139 Wroclaw, Poland; 3Institut Nationale de la Santé et de la Recherche Medicalé Unité 538, Centre Hospitalier Universitaire, St. Antoine, 75012 Paris, France; and 5Max Delbrueck Center for Molecular Medicine, D-13125 Berlin, Germany
Submitted 14 February 2003 ; accepted in final form 16 April 2003
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ABSTRACT |
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endocytosis; acute renal failure
Dependent on etiology myoglobinuria may be accompanied by additional complications but seemingly deposition of myoglobin in the kidney is a principal factor contributing to the decline of renal function. At the kidney level, the main pathological events in the genesis of myoglobinuric ARF have been identified as intense renal vasoconstriction, tubular obstruction by intraluminal pigment casts, and necrosis of proximal tubular epithelium due to direct intra- and/or extracellular toxicity of myoglobin. Because there is much evidence for the occurrence of oxidative damage to the kidney in rhabdomyolysis, the toxicity of myoglobin has been attributed to catalysis of free radical reactions. The precise mechanism, whether due to free iron, heme, or heme protein, and which free radical mechanism is involved, as well as which cellular organelles are affected, are still debated (11, 26). It has been convincingly shown that myoglobin undergoes reabsorption from the glomerular filtrate and is catabolized within proximal tubule cells (2, 14, 21). Thus it is conceivable that particular components of myoglobin can act on different intracellular levels with the involvement of diverse radical species during reabsorption of holoprotein and its subsequent decomposition. Consequently, it seems to be of great interest to elucidate the molecular mechanism of myoglobin uptake and degradation in the kidney.
Several glomerular filtrate proteins are reabsorbed as a complex with megalin and/or cubilin, the multiligand endocytic receptors residing in the membrane of proximal tubular epithelial cells. Megalin is a 600-kDa type I transmembrane glycoprotein belonging to the LDL receptor family, whereas cubilin is a 460-kDa glycoprotein that lacks a classic transmembrane domain and has no homology to any known endocytic receptors. As the vast extracellular domains of these receptors can accommodate a variety of ligands, and the receptors can act both independently or following association as a dual-receptor complex, it seems plausible that they facilitate uptake of most proteins from the primary filtrate (7). This is supported by the finding of low-molecular-weight proteinuria in megalin knockout mice and in dogs that bear an inherited disorder of intracellular cubilin processing (16, 25). Furthermore, low-molecular-weight proteinuria also develops in patients who suffer from Imerslund-Gräsback syndrome, a rare autosomal disorder caused by mutations in the cubilin gene (1).
We have recently shown that megalin and cubilin are responsible for renal reabsorption of hemoglobin, a heme protein structurally related to myoglobin (13). Thus here we aim at resolving the role of those receptors in myoglobin reabsorption.
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MATERIALS AND METHODS |
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Fluorescence-labeled myoglobin was synthetized by coupling 5(6)-carboxyfluorescein-N-hydroxysuccinimide ester (FLUOS; Roche Diagnostics, Mannheim, Germany) to myoglobin amino groups using a fluorescein-labeling kit according to the manufacturer's instructions. Briefly, 5 mg of myoglobin dissolved in PBS, pH 7.4, were incubated with 1.2 mg FLUOS for 2 h at room temperature with gentle mixing. Unbound FLUOS was removed by Sephadex G-25 gel filtration. The approximate fluorescein-to-protein ratio of the preparation was 10. Aliquots of FLUOS-myoglobin (1 mg/ml) were stored at -20°C until used.
125I-myoglobin was prepared by use of Iodo-gen according to Salacinski et al. (23). Specific activities of the tracer preparations were in the range of 1.0-1.5 µCi/µg protein.
Protein concentrations were determined using a protein assay reagent (Pierce, Rockford, IL).
Antibodies. Affinity-isolated IgG to horse myoglobin was purchased from Bethyl Laboratories (Montgomery, TX). Sheep anti-rat megalin and rabbit anti-rat cubilin antisera were obtained as described previously (15, 19). Sera IgG fractions were prepared by protein A-agarose affinity chromatography according to the manufacturer's instructions (Pierce). A nonimmune sheep serum IgG fraction was obtained from Sigma. Alexa-conjugated secondary antibodies were purchased from Molecular Probes (Eugene, OR). All other antibodies used in this study were purchased from Dako (Glostrup, Denmark).
Purification of myoglobin receptors by affinity chromatography. Rat renal brush-border membranes were prepared and solubilized using Triton X-100 as previously described (12). The membrane protein extract was recirculated at 0.2 ml/min flow through a 1.5-ml rat myoglobin-Sepharose column equilibrated with PBS, pH 7.4, 0.6 mM CaCl2, and 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS). The column was washed with 30 ml PBS, pH 7.4, 0.6 mM CaCl2, 0.5% CHAPS, and 30 ml of the same buffer containing 0.5 M NaCl, and again with 10 ml of the first buffer. Bound proteins were eluted with PBS, pH 5.0, 10 mM EDTA, and 0.5% CHAPS. Collected 1-ml fractions were concentrated 10 times by ultrafiltration using Centricon YM 10 (Millipore, Bedford, MA) and analyzed under nonreducing conditions by 4-16% SDS-PAGE. Protein bands were visualized by Gelcode blue stain reagent (Pierce). For immunochemical analysis, proteins were blotted onto Hybond ECL nitrocellulose membranes (Amersham Pharmacia Biotech, Little Chalfont, Buckingamshire, UK). Nitrocellulose membranes were blocked by 5% skim milk in 80 mM Na2HPO4, 20 mM NaH2PO4, and 0.1% Tween 20, pH 7.5 (PBS-T) for 1 h and incubated with primary antibody in PBS-T overnight at 4°C. After being washed with PBS-T, the blots were incubated with horseradish peroxidase-conjugated secondary antibody diluted 1:3,000 in PBS-T. ECL-PLUS reagent (Amersham Pharmacia Biotech) and the Fluor-s imaging system (Bio-Rad Laboratories, Hercules, CA) were used for chemiluminescent visualization.
Kinetics of myoglobin and apomyoglobin binding to cubilin and megalin. The binding of myoglobin and apomyoglobin to megalin and cubilin was studied by surface plasmon resonance analysis on a BiaCore 2000 instrument (BiaCore, Uppsala, Sweden). The procedure was essentially as described previously (5). Briefly, BiaCore type CM5 sensor chips were activated with a 1:1 mixture of 0.2 M N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide and 0.05 M N-hydroxysuccimide in water according to the manufacturer's recommendations. Megalin and cubilin were purified by RAP or IF-B12 affinity chromatography, respectively. The preparations produced single bands in SDS-PAGE followed by Coomassie brilliant blue staining. No cross-contamination of the two proteins could be detected. The proteins were immobilized at concentrations up to 50 µg/ml in 10 mM sodium acetate, pH 4.5, and the remaining binding sites were blocked with 1 M ethanolamine, pH 8.5. The resulting receptor densities were in the range of 23-40 fmol receptor/mm2. A control flow cell was made by performing the activation and blocking procedures only. Immobilized receptor proteins were reduced by injection of 0.5% dithiothreitol in 6 M guanidine hydrochloride, 5 mM EDTA, and 50 mM Tris, pH 8.0, into the flow cell. Samples were dissolved in 10 mM HEPES, 150 mM NaCl, 2 mM CaCl2, and 0.005% Tween 20, pH 7.4, or 10 mM HEPES, 150 mM NaCl, 20 mM EGTA, and 0.005% Tween 20, pH 7.4. Sample and running buffers were identical. The regeneration of sensor chips after each analysis cycle was performed with 1.6 M glycine-HCl buffer, pH 3.0. The BiaCore response is expressed in relative response units, i.e., the difference in response between proteins and the control flow channel. Kinetic parameters were determined by using BIAevaluation 3.1 software.
Competition between myoglobin and apomyoglobin for binding to receptor
sites was studied by the rapid filtration technique using isolated rat
brush-border membranes as previously described
(12). In brief, membranes (100
µg protein) were incubated in binding buffer (Tris-buffered saline) with
tracer amounts of 125I-myoglobin (104 counts/min)
and increasing concentrations of apomyoglobin in the range of 0.3-5 µM in a
final volume of 0.2 ml for 60 min. After incubation, 0.15-ml samples were
applied onto GVWP 0.22-µm membrane filters (Millipore) and washed with 5 ml
of binding buffer to remove unbound ligands. The radioactivity of the filters
corresponding to the amount of bound 125I-myoglobin was measured in
a gamma counter (Polon). Ki was evaluated by computerized
nonlinear regression analysis using Prizm software (GrapPad Software).
Immunohistochemistry. Immunohistochemical studies were performed in kidneys excised from control mice (C57BL) or kidney-specific megalin knockout mice (15) injected with myoglobin (35 mg/kg body wt in PBS, pH 7.4) into the femoral vein at 15 and 30 min after injection, respectively.
Cortical tissue specimens were prepared from kidneys after fixation with 2% paraformaldehyde in 0.1 M cacodylate buffer, pH 7.4, by retrograde perfusion through the abdominal aorta. Blocks of tissue were further fixed by immersion in the same solution for 1 h and transferred to 2.3 M sucrose in PBS, pH 7.4, for 0.5 h before being frozen in liquid nitrogen. Semithin cryosections (0.8 µm) were cut using a Reichert Ultracut S microtome (Richert-Jung, Vienna, Austria) and placed onto glass slides. Endogenous peroxidase activity was quenched with PBS, pH 7.4, 10% methanol, and 3% H2O2, and nonspecific binding was blocked with PBS, pH 7.4, 1% BSA, and 0.05 M glycine.
For the immunoperoxidase reaction, the sections were subsequently incubated with goat anti-myoglobin affinitypurified IgGs diluted 1:400-1:800 and peroxidase-conjugated rabbit IgG anti-goat IgGs diluted 1:300. The reaction was visualized with diaminobenzidine. The sections were counterstained with Meyer's hematoxilin.
In immunofluorescence studies, the following antibodies were used: goat anti-horse myoglobin affinity-purified IgGs diluted 1:400-1:800; sheep anti-rat megalin antiserum diluted 1:25,000-1:50,000; rabbit anti-rat cubilin antiserum diluted 1:2,000-1:4,000; Alexa 568-labeled donkey IgG anti-goat IgGs diluted 1:300; Alexa 488-labeled donkey IgG anti-sheep IgGs diluted 1:300; and FITC-conjugated swine IgG anti-rabbit IgGs diluted 1:40. The sections were analyzed using Leica SP2 confocal microscope.
Uptake studies in cell culture. Rat yolk sac carcinoma BN-16 cells (18) were routinely grown in 25-cm2 plastic culture flasks (Corning Costar, Badhoevedrop, Holland) in Eagle's MEM (Bio-Whittaker, Walkersville, MD) supplemented with 10% fetal calf serum (Biological Industries, Fredensborg, Denmark), 2 mM L-glutamine, 50 U/ml penicillin, and 50 µg/ml streptomycin (Bio-Whittaker) in a humidified atmosphere containing 5% CO2 at 37°C. Cells were passaged every fourth day at a split ratio of 1:5 by trypsinization with 500 mg/l trypsin and 200 mg/l EDTA (Bio-Whittaker).
For uptake experiments, cells were cultured in eight-chamber glass slides (Nalge Nunc International, Naperville, IL). One day before the cells reached confluence, the medium was replaced with medium supplemented with 0.5% ovoalbumin instead of 10% serum (serum-free medium). Cell monolayers were incubated with 5 µg/ml FLUOS-myoglobin in serum-free medium for 10 min and fixed with 1% paraformaldehyde in PBS, pH 7.4, for 3 min. For inhibition studies, the following proteins were added to the incubation mixture: 1 µM recombinant receptor-associated protein, a 200-mg/l sheep anti-rat megalin IgG serum fraction and a sheep nonimmune IgG serum fraction, or 400-mg/l rabbit anti-rat cubilin IgG serum fraction and rabbit nonimmune IgG serum fraction. The slides were mounted with 50% glycerol, 2% N-propyl-gallat, and 2.4% Tris and examined using a fluorescence microscope (Leica DMR) equipped with a color video camera (Sony 3CCD).
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RESULTS |
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Surface plasmon resonance analysis. Kinetic parameters of myoglobin binding to megalin or cubilin were examined by means of surface plasmon resonance analysis. Kd values for the interactions of myoglobin with megalin or cubilin were 2.0 and 3.0 µM, respectively (Fig. 2, A and B). Apomyoglobin bound to megalin with the same affinity as myoglobin (Kd = 2 µM), whereas the affinity of apomyoglobin for cubilin was lower (Kd = 5 µM). The binding of both myoglobin and apomyoglobin was completely abolished in the presence of EDTA (Fig. 2, C and D). Competition of both myoglobin forms for binding sites on the receptors was tested in an inhibition study using isolated brush-border membranes. Radioiodinated myoglobin could be displaced by apomyoglobin with a Ki of 2 µM (Fig. 3).
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Uptake of myoglobin in vitro. The role of megalin and cubilin in the endocytic uptake of myoglobin was investigated by an inhibition study in cultured BN-16 cells, a cell line derived from the rat yolk sac. The cells could intensively internalize FLUOS-myoglobin from the incubation medium, which appeared as green fluorescence accumulating within the endosomal/lysosomal compartment of the cell. The uptake was site limited because an excess of unlabeled myoglobin (20 µM) virtually prevented fluorescent labeling (not shown). We observed a marked inhibition of FLUOS-myoglobin uptake with anti-megalin or anti-cubilin antibodies at a concentration of 200 and 400 µg/ml, respectively, or with 1 µM RAP (Fig. 4).
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Uptake of myoglobin in vivo. The significance of megalin- and cubilin-mediated endocytosis for myoglobin reabsorption in vivo was investigated by immunocytochemistry in kidney sections from normal and kidney-specific megalin knockout mice (15). Because endogenous myoglobin could be detected in neither normal mouse nor knockout mouse proximal tubule (data not shown), we performed our experiments using mice injected with exogenous myoglobin at a dose of 35 mg/kg body wt. Thirty minutes after injection, a strong immunoperoxidase reaction with anti-myoglobin antibodies was identified mainly along the brush border and within the apical endosomal/lysosomal compartment in virtually all cells of the proximal tubule from normal mice. There was also additional immunoreactivity in the extratubular space (Fig. 5A). In the megalin knockout mouse, some proximal tubular cells were devoid of immunoreactivity, whereas in others the distribution of the immunoreaction was analogous to that in the normal mouse (Fig. 5B). To demonstrate a relationship between the expression of megalin or cubilin and reabsorption of myoglobin in those two subsets of cells, we employed the double-immunofluorescence technique. Normal and deficient cells could be easily distinguished by anti-megalin antibodies. There was a pronounced reduction of cubilin expression in the cells lacking megalin. Both receptors colocalized mainly in the apical membrane area (Fig. 6). Megalin-immuno-reactive cells exhibited substantial deposition of myoglobin at the brush border and in the apical endosomal/lysosomal apparatus as well as in vesicular structures located deeper in the cell. Myoglobin and megalin or cubilin, respectively, colocalized at the brush border and in vesicles adjacent to the apical plasma membrane. Uptake of myoglobin by the cells devoid of megalin was either very low or not seen at all.
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DISCUSSION |
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To clarify whether these relatively weak interactions can genuinely promote the uptake of myoglobin, we employed a cell culture model in our studies. Because in cultured cells of proximal tubule origin like LLCPK1 or opossum kidney cells the expression of megalin and cubilin is much lower compared with those in vivo, we chose BN-16 cells for these experiments. BN-16 cells, originating from yolk sac epithelium, are structurally and functionally similar to renal proximal tubule cells but exhibit high rates of cubilin- and megalin-mediated endocytosis. These cells have previously been used as an in vitro model of proximal tubular endocytosis (17, 18). Significantly, accumulation of fluorescence-labeled myoglobin in the cells was inhibited by antibodies raised against purified receptors and by RAP, a chaperone that affects binding of most megalin and cubilin ligands (6).
To assess the role of the receptors in the clearance of myoglobin in vivo, we performed experiments using kidney-specific megalin knockout mice. The mice were designed specifically to conquer the problems of high perinatal lethality and severe complex phenotype associated with full megalin gene knockout, so far limiting the usefulness of the model. Renal specific knockout mice present a severe tubular reabsorption deficiency associated with up to a 90% reduction in the number of proximal tubular cells expressing megalin but exhibit normal development and viability (15). Mice were injected intravenously with myoglobin at a dose of 35 mg/kg, which is compatible with the concentration range observed in rhabdomyolysis (3). A comparison of myoglobin deposition in megalin-deficient and normal cells after intravenous administration of the protein clearly implied that megalin-mediated endocytosis is a predominant route of myoglobin entry into proximal tubule cells. In normal cells, myoglobin colocalized with the receptors at the brush border and within the apical vacuolar apparatus, which reflected its binding to the receptors and subsequent endocytosis. Part of the endocytosed myoglobin could be detected in vacuolar structures devoid of the receptors, which is consistent with early sorting of the receptors into the recycling compartment (8). Deposition of injected myoglobin in megalin-deficient cells was strikingly reduced and limited to relatively small vacuolar structures, which resembled uptake by fluid-phase endocytosis. This process has been previously characterized in the proximal tubule as a minor nonreceptor endocytic transport system accounting for <2% of the total uptake (9). Besides the epithelium, there was also deposition of myoglobin in the interstitium, indicating a substantial leak of the protein through the capillary walls. The finding possibly discloses one more potential target of myoglobin toxicity in the kidney.
Necrosis of proximal tubular epithelium is a trait/histopathological phenomenon found in the myoglobinuric kidney and thus is believed to be an important factor in the development of the disease. Substantial evidence has linked this lesion to iron-driven oxidative stress in connection with intracellular breakdown of the hemoprotein (26). In light of our findings, one could envisage that restriction of megalin-mediated endocytosis of myoglobin, especially early after rhabdomyolytic insult, could have a beneficial effect on myoglobinnuric ARF. A potential competitor of myoglobin endocytosis that emerged during this study was apomyoglobin. Lacking the neprotoxic heme, apomyoglobin can displace myoglobin from the receptor-binding sites. However, it is not known to what extent such competition can occur in vivo and whether administration of the doses required for inhibition would have its own adverse effects.
In conclusion, our study establishes a molecular mechanism of myoglobin uptake in the renal proximal tubule involving the endocytic receptors megalin and cubilin. Our findings may offer a perspective for experimental therapies preventing development of myoglobinuric ARF. We suggest that organ-specific megalin knockout mice may be employed in further studies on the pathophysiology of myoglobinuric ARF. Such investigations may in particular clarify the significance of tubular myoglobin reabsorption for the development of ARF as well as its role during the maintainance and recovery phase of this disease.
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DISCLOSURES |
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ACKNOWLEDGMENTS |
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FOOTNOTES |
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The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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REFERENCES |
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