Third Department of Internal Medicine, University of Occupational and Environmental Health, Japan, School of Medicine, Kitakyushu 807, Japan
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ABSTRACT |
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Plasma
cholecystokinin (CCK) levels in fed rats increased from 2.59 ± 0.13 pmol/l to the peak of 27.6 ± 4.1 pmol/l at 1 h after a single oral
administration of synthetic protease inhibitor (PI; ethyl
N-allyl-N-{(E)-2-methyl-3-[4-(4-amidino-phenoxycarbonyl)phenyl]propenoyl}amino acetate methansulfonate; 20 mg/kg body wt), but then returned to the
preloading value at 12 h after administration. The pancreatic fluid
secretion, rich in chloride but poor in bicarbonate, was significantly
elevated at 6-12 h postfeeding (100.9 ± 8.2 vs. 27.3 ± 2.3 µl/30 min in control rats, P < 0.01). Loxiglumide (50 mg · kg body
wt1 · h
1),
atropine (100 µg · kg body
wt
1 · h
1),
or antisecretin serum (100 µl/rat) at 12 h postfeeding did not modify
the fluid hypersecretion. Loxiglumide, when given together with PI,
completely abolished fluid hypersecretion, but it could not inhibit
hypersecretion when applied 3 h after PI treatment. Labeling with
5-bromo-2'-deoxyuridine showed active proliferation of acinar
cells at 3 h after PI treatment (3.56 ± 0.29% vs. 0.46 ± 0.08% in control, P < 0.001), but
not in rats given loxiglumide together with PI. In rats that fasted
from 12 h before to 12 h after PI feeding, neither pancreatic fluid
hypersecretion nor active proliferation of acinar cells was observed.
These results suggest that pancreatic fluid hypersecretion in fed rats
at 6-12 h after PI treatment is caused not by CCK-, secretin-, or
cholinergic-dependent mechanisms but probably by acinar cell
proliferation.
acinar cell proliferation; cholecystokinin antagonist; endogenous cholecystokinin release
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INTRODUCTION |
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IT IS WELL KNOWN THAT the presence of trypsin and chymotrypsin in the proximal small intestine exerts a negative feedback regulation on pancreatic exocrine secretion in rats (5, 8, 16, 20, 40), guinea pigs (15), pigs (11), and humans (13, 19, 22, 32, 33). Inhibition of luminal proteolytic activities by intraduodenal infusion of soybean or synthetic trypsin inhibitors (6, 8, 19, 30, 31, 40) or intact protein (17, 18) or by diversion of biliary-pancreatic secretions away from the small intestine (5, 8, 20) has been shown to increase plasma concentrations of cholecystokinin (CCK) (6, 16, 30, 40) and secretin (31, 40) and to strongly stimulate pancreatic exocrine secretion (6, 16, 30, 40). In our previous studies (29, 30), we have demonstrated that an oral administration of the synthetic trypsin inhibitor camostat mesylate has two different stimulatory effects, immediate and delayed, on pancreatic fluid secretion. Oral or intraduodenal administration of camostat mesylate stimulates an increase in plasma levels of endogenous CCK (6, 30, 31, 40), irrespective of whether rats were fed or fasted, causing an immediate pancreatic exocrine secretion. The immediate pancreatic fluid hypersecretion occurred within 30 min, whereas the delayed hypersecretion was observed only in fed rats at 6-24 h (peak increase at 12 h) after camostat feeding (29, 30). Moreover, the early effects of camostat on pancreatic exocrine secretion were abolished in in vitro isolated perfused pancreas, in which humoral and neural influences on the pancreas are eliminated, whereas the late effects were still observed in the absence of humoral and neural influences (29, 30).
The present experiments were designed therefore to further clarify the underlying mechanism of the delayed pancreatic fluid hypersecretion after camostat treatment. In the present study, we used a newly synthesized protease inhibitor (PI), ONO-3403, which is orally active and, in comparison to camostat, is 5-10 times more potent in inhibiting protease activities and has a longer duration of action (35).
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MATERIALS AND METHODS |
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Materials
Synthetic PI (ethyl N-allyl-N-{(E)-2-methyl-3-[4-(4-amidino-phenoxycarbonyl)phenyl]propenoyl}amino acetate methansulfonate; ONO-3403) was a generous gift from Ono Pharmaceutical (Osaka, Japan). A potent and specific CCK-A receptor antagonist, loxiglumide, was obtained from Kaken Pharmaceutical (Tokyo, Japan).We purchase the following materials: cholinergic receptor antagonist atropine sulfate from Nacalai Tesque (Kyoto, Japan), antisecretin rabbit serum from Peptide Research Institute (Osaka, Japan), 5-bromo-2'-deoxyuridine (BrdU) labeling and detection kit II from Boehringer Mannheim Biochemica (Mannheim, Germany), and DST800 multititration system from Radiometer (Copenhagen, Denmark).
Animals and Treatments
Male Wistar rats weighing 220-250 g were used in all experiments. The animals were kept at 22°C on a 12:12-h light-dark cycle with free access to water and a standard laboratory diet containing 53% carbohydrates, 25% protein, and 6% fat (Oriental Yeast, Tokyo, Japan). PI dissolved in 0.15 M NaCl (20 mg · 2 mlSecretory Studies
PI was given to awake rats by an orogastric tube, and rats were kept with free access to water and a standard laboratory rat diet until surgery. Pancreatic exocrine function was studied at specified time points after oral administration of PI by the method described in detail previously (38). Briefly, rats were laparotomized under urethan anesthesia (1.6 g/kg body wt sc). The proximal end of the bile duct was ligated near the liver, and the bile was drained into the duodenum through a polyethylene tube inserted into the bile duct. The distal end of the common bile-pancreatic duct was cannulated near its entrance into the duodenum. The left jugular vein was cannulated, and the pylorus was ligated. Pancreatic fluid secretion was obtained by replacing a calibrated tube attached to the free end of the pancreatic cannula every 30 min, and the volume, the protein output, and the bicarbonate and chloride concentrations were determined. The abdominal wound was covered with a saline-moistened gauze, and the body temperature was maintained at 36-37°C with a heating pad throughout the experiment.Experimental Protocol
Basal pancreatic secretion.
The basal pancreatic fluid secretion was collected for 60 min, and
fluid volume, protein output, bicarbonate and chloride concentrations
were determined before and 3, 6, and 12 h after oral PI administration.
To examine the underlying mechanism of the fluid hypersecretion in
PI-treated rats, the following experiments were performed in rats at 12 h after oral PI administration. In these experiments, PI was given at
12:00 AM to awake rats by an orogastric tube, and rats were kept with
free access to water and a standard laboratory rat diet.
Surgery was started at 10:30 AM, and pancreatic fluid secretion was
collected for 120 min, from 11:00 AM to 1:00 PM. First, a
potent and specific CCK-A receptor antagonist, loxiglumide, at a dose
of 50 mg · kg body
wt1 · h
1,
or the cholinergic receptor antagonist atropine sulfate at a dose of
100 µg · kg body
wt
1 · h
1
was intravenously infused at a rate of 1 ml/h for 60 min, from 12:00 to
1:00 PM. Pancreatic fluid was collected from 60 min before to 60 min
after the commencement of the antagonist infusion. This dose of
loxiglumide (26) or atropine (23) can effectively suppress basal or
stimulated pancreatic fluid secretion. In addition, antisecretin rabbit
serum was administered by intravenous bolus injection at a dose of 100 µl at 12:00 PM to immunoneutralize the endogenous secretin, and
pancreatic fluid was collected from 60 min before to 60 min after the
injection. This dose completely abolishes the pancreatic
exocrine secretion stimulated by intravenous infusion of 1.0 U · kg body
wt
1 · h
1
secretin (39). Any cross-reactions against human gastrin-releasing peptide, human gastrin, human glucagon, and somatostatin are not observed at much higher doses (1-10 nmol/ml). This antibody showed 100% cross-reactivity with human and porcine secretin (according to
communication with the manufacturer). Second, the CCK-A receptor antagonist loxiglumide at a dose of 50 mg/kg body wt sc was given together with oral administration of PI. Third, the same dose of
loxiglumide was subcutaneously given at 3 h after oral PI
administration.
Cellular source of fluid hypersecretion. We then investigated the cellular source of the fluid hypersecretion in the PI-treated rats. Because pancreatic acinar cells produce chloride-rich fluid whereas pancreatic ductal cells produce bicarbonate-rich fluid (37), chloride and bicarbonate concentrations in the pancreatic fluid were determined. In addition, in vivo labeling of proliferating cells with BrdU was determined. One hour before the rats were killed, 10 mmol/l BrdU labeling reagent (1 ml/100 g body wt) was intravenously injected into both control and PI-treated rats to label proliferating cells (7).
Histological Examination
A portion of the pancreatic tissue was fixed for 24 h in a 10% formaldehyde solution for hematoxylin and eosin staining for light microscopy examination. To identify proliferating cells, BrdU incorporated into replicating DNA was stained using a specific monoclonal antibody. At least 1,000 acinar cells and 300 ductal cells were counted. The labeling index was considered to be the number of BrdU-positive cells per 100 counted cells.Assays
Protein and bicarbonate concentrations in the pancreatic fluid were determined by the method of Lowry et al. (21) and by the DST800 multititration system (34), respectively. Chloride concentration in pancreatic fluid was determined according to the method of Schales and Schales (34). CCK concentrations in plasma were measured by a sensitive and specific radioimmunoassay, using antiserum OAL-656 with CCK-8 as a standard (36).Data Analysis
Results were expressed as means ± SE. Statistical analysis was performed by using the Mann-Whitney U test. Paired data were analyzed using a paired t-test. Differences with a P value of <0.05 were considered statistically significant. ![]() |
RESULTS |
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Plasma CCK Levels after PI treatment
In fed rats, plasma CCK concentrations increased significantly from the basal value of 2.59 ± 0.13 pmol/l to the peak of 27.6 ± 4.1 pmol/l 60 min after an oral administration of 20 mg/kg body wt PI, but then decreased to 15.8 ± 2.6 pmol/l after 2 h and returned to the preloading levels after 12 h (2.93 ± 0.24 pmol/l) (Fig. 1).
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PI also caused a significant increase in plasma CCK levels in fasted rats from the basal levels of 1.48 ± 0.12 pmol/l to the peak of 10.91 ± 1.56 pmol/l after 30 min and returned to the preloading levels after 4 h (1.77 ± 0.88 pmol/l). However, plasma CCK levels in fasted rats were significantly lower than those in fed rats at all corresponding time points (Fig. 1).
Basal Pancreatic Fluid and Protein Output
Oral administration of PI had no influence on basal fluid secretion at 3 h after, but it caused a significant increase in pancreatic fluid secretion at 6 h after (61.0 ± 9.6 vs. 27.3 ± 2.3 µl/30 min in control rats, P < 0.01) and further increased pancreatic fluid secretion at 12 h after (Table 1, Fig. 2). PI also increased protein output at 12 h after (Table 1, Fig. 2). The pancreatic fluid was rich in chloride but poor in bicarbonate concentration (Table 1). In contrast to fed rats, basal pancreatic fluid, protein output, and bicarbonate and chloride concentrations in fasted rats were similar to those in the control rats (Table 1).
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Mechanism of Pancreatic Fluid Hypersecretion
Because the basal fluid secretion was elevated in PI-treated fed rats, we examined the underlying mechanism in rats at 12 h postfeeding. Intravenous infusion of the CCK-A receptor antagonist loxiglumide at 12 h post-PI treatment at a dose of 50 mg · kg body wt
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Intravenous infusion of the cholinergic receptor antagonist atropine at
a dose of 100 µg · kg body
wt1 · h
1
was also unable to modify the fluid and bicarbonate secretion, but it
significantly suppressed protein output (Table 2). Similarly, antisecretin serum had no significant influence on fluid
hypersecretion, although it further decreased bicarbonate concentration
from 41.9 ± 3.0 to 22.0 ± 3.0 mmol/l (Table 2).
Cellular Source of Fluid Hypersecretion
Chloride and bicarbonate concentrations in pancreatic fluid. PI treatment increased chloride concentration, whereas it reciprocally decreased bicarbonate concentration with time (Table 1, Fig. 3). These electrolyte changes in the pancreatic fluid were observed before the increase in fluid secretion, as early as 3 h after PI feeding (Figs. 2 and 3).
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Labeling of proliferating cells. The proliferating cells labeled with BrdU were seen mainly in acinar cells. The labeling index of acinar cells showed a 7.7-, 2.7-, and 0.6-fold increase above the control value at 3, 6, and 12 h, respectively, after PI treatment. Active proliferation of epithelial cells of the interlobular pancreatic duct showed only a 2.6-, 2.5-, and 1.3-fold increase above control at 3, 6, and 12 h, respectively, after PI treatment (Table 3, Fig. 4). In contrast, the labeling indexes of acinar and duct epithelial cells in rats given loxiglumide together with PI were slightly lower, although statistically not significant, than those in the controls (Table 3).
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DISCUSSION |
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The results of the present study have confirmed our earlier studies (29, 30) demonstrating that a single oral PI administration causes pancreatic fluid hypersecretion only in fed rats at 6-12 h postfeeding and have extended our previous understanding by revealing that the fluid hypersecretion is not under the regulation of CCK-, secretin-, or cholinergic-dependent mechanisms.
Previous studies have demonstrated that a single oral dose of PI causes an increase in plasma CCK concentrations and pancreatic exocrine secretion for more than 2 h (14, 29, 31). However, the expected increase in basal pancreatic exocrine secretion in rats that had been given PI 3 h before was not seen, but the basal pancreatic fluid secretion was increased 6-12 h postfeeding. These results, together with previous observations (29, 30), suggest different mechanisms between the early and the late response after PI feeding.
There are several possible explanations for pancreatic fluid hypersecretion in rats late after oral administration of PI. It is commonly accepted that the exocrine secretion of the pancreas is regulated by a CCK-mediated negative-feedback mechanism, which is triggered by lowering of the duodenal concentration of proteolytic enzymes either by diversion of biliary-pancreatic secretions away from the small intestine (5, 8, 20) or by infusion of trypsin inhibitors (6, 8, 30, 31, 40) or intact protein into the small intestine (18). This feedback mechanism is thought to be mediated by endogenous CCK-releasing peptides in the lumen. One such peptide, monitor peptide (MP), is secreted by the pancreas into pancreatic juice (12). The others appear to be secreted by the intestine into the lumen (22, 24) and have been identified as "luminal CCK-releasing factor," or LCRF (36), and "diazepam-binding inhibitor," or DBI (10). These peptides are all trypsin sensitive and exert CCK-releasing action by avoiding trypsin degradation in the presence of intraluminal trypsin inhibitors or food that transiently binds trypsin. One possible explanation for the delayed increase in pancreatic exocrine secretion after an oral dose of PI is that PI reduces intraduodenal activities of proteolytic enzymes (6, 30, 40) or acts directly on CCK-producing cells in the small intestinal mucosa, resulting in a significant increase in CCK (2, 17), which stimulates pancreatic enzyme secretion including MP as well as trypsin and chymotrypsin into the duodenum. In a fasted state, these proteolytic enzymes presumably hydrolyze and inactivate MP, LCRF, or DBI and thus inhibit further release of CCK, whereas these CCK-releasing peptides can survive intact in fed rats because dietary protein in the duodenum competes for the trypsin and chymotrypsin and thus continuously stimulates the release of CCK. If, however, the prolonged increase of endogenous CCK were related to the delayed hypersecretion, the potent and highly specific CCK-A receptor antagonist loxiglumide (50 mg/kg body wt) (26, 27) might inhibit pancreatic fluid hypersecretion seen at 12 h postfeeding. Moreover, if endogenous CCK were related to the delayed hypersecretion, fluid secretion might be increased in rats given loxiglumide together with oral PI, because plasma CCK levels at 12 h after PI treatment in these rats were significantly higher than those in rats given PI alone. Because the plasma CCK levels at 12 h after PI treatment were similar to the preloading basal levels and since intravenous infusion of loxiglumide was unable to suppress the fluid hypersecretion, it is unlikely that the pancreatic fluid hypersecretion is caused by the direct effect of endogenous CCK.
The possibile effect of cholinergic tone on the fluid hypersecretion is
also refuted by the ineffectiveness of atropine in inhibiting the fluid
hypersecretion. On the other hand, immunoneutralization of circulating secretin slightly but not significantly suppressed the
pancreatic fluid hypersecretion and greatly decreased bicarbonate concentration (47%). This provides clear evidence that
endogenous secretin does not play an important role in fluid
hypersecretion in PI-treated rats. In support of this view, the
camostat-induced basal pancreatic fluid hypersecretion is observed even
in in vitro perfused pancreas, in which humoral and neural influences
are eliminated, while the circulatory system is maintained via intact arteries and veins (29, 30). Taken together, it is unlikely that any
humoral and neural factors are responsible for the basal fluid
hypersecretion in rats at 12 h after PI feeding.
Subcutaneous administration of loxiglumide together with oral PI
completely abolished fluid hypersecretion, but it had no inhibitory
effects when given 3 h after PI feeding. On the other hand, intravenous
infusion of atropine at a dose of 100 µg · kg body
wt1 · h
1
for 60 min starting immediately after an oral administration of PI had
no influence on fluid hypersecretion at 12 h postfeeding (unpublished observations). These results suggest that the fluid hypersecretion is related to the early changes in the exocrine pancreas
induced by endogenous CCK release at a very early time point after PI
feeding (within 3 h). It is conceivable therefore that some functional
changes in the exocrine pancreas, which can be prevented by cotreatment
but cannot be suppressed by posttreatment with loxiglumide, might have
occurred in the pancreas after oral administration of PI, leading to a
persistent fluid hypersecretion.
The time course of the fluid hypersecretion appears to agree with the proliferating process of acinar cells. This process is probably triggered by complex mechanisms involving gastrointestinal hormones such as CCK and secretin. Among them, CCK is thought to be the most important (25). The proliferative process might be triggered by the initial increase in endogenous CCK. Thus the subsequent blockade of CCK might have no effect, because the increased proliferation of acinar cells is already underway and does not require CCK for maintenance, as we have shown in our recent studies that maintenance of hyperplasia requires less CCK than induction of hyperplasia (25, 28). Indeed, the labeling index of acinar cells with BrdU showed a 7.7-fold increase above the control value at 3 h after, and subcutaneous injection of loxiglumide at this time point could not inhibit the increase. Moreover, the labeling index of acinar cells in rats given loxiglumide together with oral PI was lower than that at control levels, and the pancreatic fluid hypersecretion was completely abolished in this group of rats.
An increased mitotic activity after PI treatment was seen as early as 3 h in both the acinar and ductal cells. In accordance with this proliferative process, chloride concentration in pancreatic fluid was significantly increased, whereas bicarbonate concentration was markedly decreased, indicating an acinar source of the fluid. The increases in protein output as well as fluid volume and chloride concentration in the PI-treated rats further suggest the acinar source of the fluid hypersecretion. However, because a simultaneous increase in acinar and ductular proliferation is seen, it is possible that not only acinar but also ductal cells contribute to fluid hypersecretion in the PI-treated rats. Although secretin is known to cause pancreatic fluid secretion mainly from the duct system and to increase bicarbonate concentration with a reciprocal fall in chloride concentrations (1), PI feeding decreased bicarbonate concentration and increased chloride concentration with an increase in fluid secretion. Acinar cell proliferation appears to be the source of the increase in CCK-, secretin-, and cholinergic-independent pancreatic fluid hypersecretion in the PI-treated rats.
Similar to PI-pretreated rats, we found in our recent study that basal pancreatic fluid secretion, rich in chloride but poor in bicarbonate, is greatly increased at 24 h after the commencement of caerulein-induced pancreatitis (fluid vol, 105.1 ± 4.6 µm/30 min) (3). The labeling index with BrdU of acinar cells in these pancreatitic rats showed a 12.1-fold increase above the control levels at 6 h but decreased to the preloading level at 24 h after. In the present study, the labeling index of acinar cells peaked at 3 h, whereas fluid hypersecretion was observed later than 6 h after PI treatment. Taken together, these results suggest that acinar cells that have progressed beyond the synthesis period cause pancreatic fluid hypersecretion, because BrdU is specifically incorporated into the cells in the synthesis period of the cell cycle (7).
In contrast to fed rats, in fasted rats PI did not cause any significant increase in the basal pancreatic fluid secretion, as we have demonstrated in our previous study (30). However, oral administration of PI in fasted rats caused an eightfold increase in plasma CCK levels, as was the case in the fed rats (Fig. 1). Because the rats were fasted from 12 h before to 12 h after PI administration, the absence of fluid hypersecretion can be explained by a deficit of substrates (amino acids) or energy (calories) for protein synthesis in the pancreas. Cell proliferation requires energy and substrates for protein synthesis, and depletion of either can slow or prevent replication. Indeed, recent studies have demonstrated that a low-protein diet or the absence of food in the gastrointestinal tract decreases the trophic effects of both exogenous and endogenous CCK on the rat pancreas (4, 9). Thus oral intake of adequate protein is important to induce the full effect of PI.
In conclusion, the present study demonstrated that the pancreatic fluid hypersecretion seen after PI treatment is not under the regulation of a CCK-, secretin-, or cholinergic-dependent mechanism. Acinar cell proliferation is the most probable source of the agonist-independent fluid hypersecretion.
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FOOTNOTES |
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Address for reprint requests: M. Otsuki, Third Dept. of Internal Medicine, Univ. of Occupational and Environmental Health, Japan, School of Medicine, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807, Japan.
Received 20 June 1997; accepted in final form 13 November 1997.
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