Department of Surgery, Tohoku University School of Medicine, Sendai, Japan 980-8574
![]() |
ABSTRACT |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Normal luminal bacteria and their products play a role in experimental colitis and inflammatory bowel disease. However, what molecules from what cells are responsible for mounting and maintaining the mucosal defense against luminal flora is still uncertain. The aim of this study was to identify epithelial gene products involved in mucosal defense and inflammation associated with ubiquitous enteric bacteria. Germ-free ICR mice were given an oral bacterial suspension prepared from conventional components (bacterial reconstitution). Small intestinal and colonic epithelial cells were isolated from bacteria-reconstituted, germ-free, and specific pathogen-free mice. Differential gene expression was investigated by differential display, Northern blot, and sequence analysis. Bacterial reconstitution resulted in acute but self-limited colitis. In epithelial cells, we observed the induction of small intestine-specific genes of the cryptdin family and colon-specific expression of serum amyloid A1 gene. This novel approach allows the identification of known and novel gene products involved in mucosal defense against luminal microorganisms and the associated inflammatory response.
germ-free mice; intestinal epithelial cells; mucosal inflammation; differential display
![]() |
INTRODUCTION |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
INFLAMMATORY BOWEL DISEASE (IBD) is a disease of unknown etiology. Current theories suggest that both Crohn's disease and ulcerative colitis are multifactorial entities conditioned by genetic, environmental, microbial, and immunological factors (7). It is widely accepted that luminal bacteria and their products play a role in both forms of IBD (3) and that components of the normal intestinal flora can trigger, initiate, or contribute to IBD pathogenesis (7). This concept is supported by the demonstration of IBD-like lesions in various animal models of experimental IBD, such as indomethacin-induced enterocolitis or carrageenan-induced or dextran sulfate sodium-induced colitis (21, 25, 35). Commensal intestinal bacteria appear to play a key role in the development and exacerbation of chemically induced colitis. Moreover, chronic intestinal inflammation also develops in genetically engineered animals such as interleukin (IL)-2, IL-10 knockout mice or HLA-B27 transgenic rats only in the presence of luminal bacteria and not under germ-free (GF) conditions (11, 26, 30). Together, these reports clearly show that commensal intestinal bacteria are intimately involved in the development of mucosal inflammation when inciting or genetic abnormalities are present. However, in such models, the molecules that are responsible for the development and perpetuation of mucosal inflammation, and the cellular source of these molecules, have not been identified. It is reasonable to assume that the mechanism of mucosal inflammation depends, at least in part, on the interaction of commensal luminal bacteria associated with mucosal cells, particularly epithelial cells, which are in close physical contact with the bacteria. Therefore, identification and characterization of the molecules associated with conventional bacteria-induced inflammation and recovery is critically important for the understanding of any type of experimental, or perhaps clinical, form of IBD. Identification of essential molecules involved in the mucosal defense system would also be indispensable in establishing whether abnormalities found in IBD mucosa are primary or secondary events caused by the existence of bacteria associated with IBD pathogenesis. This could lead to a better therapeutic approach by controlling secondary inflammatory responses even if the etiology of IBD has not been fully elucidated.
Because of the lack of experimental systems, only limited information is available regarding normal antibacterial responses in the gut. Mucosal cells in genetically "normal" animals without obvious inflammation-inducible agents may develop antibacterial responses via a well-balanced expression of specific gene products to create and maintain a mucosal defense system. Molecules participating in mucosal defense against luminal bacteria are probably multiple, including cytokines, receptors, cytoskeleton proteins, and enzymes, among others. In addition, it is likely that differential antibacterial mechanisms are present in the colon and small intestine.
In this study, we have established a novel approach for analysis of mucosal defense mechanisms against nonpathological luminal microorganisms. This approach enables us to understand better the epithelial cell response induced by the enteric flora via the examination of the induction or suppression of gene products not previously known to be specifically involved in gut mucosal defense and inflammation.
![]() |
MATERIALS AND METHODS |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Animals. GF and specific pathogen-free (SPF) ICR mice were purchased from Clea Japan (Tokyo, Japan). Fecal suspensions were freshly prepared from SPF mice by 10-fold dilution of colonic content with saline. GF mice at 6, 7, and 8 wk of age (n = 8 per age group) were given intestinal microorganisms via oral administration of fecal suspensions and were reared with SPF mice under SPF conditions. Bacteria-reconstituted 8-wk-old mice were killed after 3, 7, and 14 days by cervical dislocation. Non-bacteria-reconstituted GF (n = 8) and SPF (n = 8) mice were used as controls and were also killed at the age of 8 wk. This protocol was approved under the regulations in the Guide for the Care and Use of Laboratory Animals of Tohoku University School of Medicine.
Tissue sampling for histology and serological assessment. Tissues from the ileum, cecum, and rectum were obtained in each group, immediately fixed in 10% buffered formalin, and embedded in paraffin wax. Three-micrometer-thick sections were stained with hematoxylin-eosin. Albumin concentration in serum, glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), and lactate dehydrogenase (LDH) activity and C-reactive protein (CRP) were determined using Spotchem (KDK, Kyoto, Japan).
Isolation of intestinal and colonic epithelial cells in mice. The small intestine and colon were treated separately and washed with Hanks' balanced salt solution (HBSS). Epithelial cells were isolated as previously described elsewhere with minor modifications (13). Briefly, small intestine or colon tissue was cut into small pieces ~2-3 cm in length and incubated in HBSS with 1.5 mg/ml dithiothreitol (DTT) for 10 min at room temperature. Tissue pieces were then put into HBSS with 1 mM EDTA at room temperature for three 10-min periods. At the end of each period, they were shaken rapidly with a glass rod. The supernatants were collected by centrifugation, and the resulting pellets were washed twice in RPMI 1640. Viability of purified epithelial cells was >95%.
RNA preparation. Total RNA was extracted from isolated epithelial cells as described previously (28). Briefly, epithelial cells were dissolved in guanidine isothiocyanate buffer followed by a 5.7 M cesium chloride gradient. The quantity and quality of RNA were confirmed by 260-nm ultraviolet absorbance and gel electrophoresis with ethidium bromide staining, respectively.
Differential display. Differential display was performed as previously described (14). cDNA subpopulations were generated from 2.5 µg of epithelial total RNA using reverse transcriptase (Superscript II; GIBCO, Grand Island, NY) with oligo GT15X (X = A, G, or C) primers in 20 µl of reverse transcription buffer supplied by the manufacturer. Oligo GT15X primers were synthesized with DNA/RNA synthesizers (Applied Biosystems, Foster City, CA). The resulting cDNA solutions were diluted fivefold in Tris-EDTA buffer. PCR was performed on each of the cDNA subpopulations with 10-bp upstream primers (Operon 10-mer kits; Operon, Alameda, CA). Two microliters of diluted cDNA solutions were added to a mix with 0.5 units of Taq DNA polymerase (Amersham Japan, Tokyo, Japan), 0.5 units of Gene Taq (Nippon Gene, Toyama, Japan), 0.2 mM nucleotides (dATP, dGTP, dCTP, and dTTP), 0.5 µM appropriate oligo GT15X, 0.5 µM upstream primers, and Gene Taq buffer supplied by the manufacturer in a total volume of 20 µl for 30 cycles with a Robocycler thermocycler (Stratagene, La Jolla, CA). Each cycle consisted of 94°C for 30 s, 42°C for 2 min, and 72°C for 1 min. PCR products were identified by staining with SYBR Green I (FMC, Rockland, ME) after electrophoresis on 6% acrylamide gels, and photographs were taken. Bands were selected on the basis of whether 1) bands were induced or suppressed by bacterial reconstitution, 2) bands were differentially modulated between small intestine and colon, and 3) bands were small intestine or colon specific.
Cloning and sequencing. The gel piece containing the band of interest was excised on a ultraviolet illuminator. The DNA was reamplified by the direct addition of the gel to an appropriate 100-µl PCR reaction and 20-25 cycles of PCR. The reamplified fragments of DNA were fractionated on 1% agarose gels, excised, and purified by PCR preps DNA purification system (Promega, Madison, WI). The cDNA was subcloned into a T vector (pGEM-T Easy Vector System; Promega) and sequenced using a Cy5 Auto Cycle sequencing kit (Pharmacia Biotech, Tokyo, Japan) and an ALFred DNA sequencer (Pharmacia Biotech). The subcloned cDNA was used as a probe for Northern blot analysis.
Northern blot analysis. Total RNA (20 µg) was fractionated by electrophoresis on 1% agarose-formaldehyde gels and transferred onto a nylon transfer membrane (Amersham, Tokyo, Japan). The protocol for Northern blotting was described previously (9).
Detection of murine cryptdin isoform.
cDNA was synthesized using oligo (dT)12-18 (GIBCO BRL)
as a primer for the reaction. Cryptdin-1, -4, and -5 cDNAs were amplified using the consensus forward primer Defcrp130
(5'-AAGAGACTAAAACTGAGGAGCAGC-3') paired with cryp1
(5'-CGACAGCAGAGCGTGTA-3') for cryptdin-1, cryp4 (5'-CGGCGGGGGCAGCAGTA-3') for cryptdin-4, and cryp5
(5'-GCAGCAGAATACGAAAGT-3') for cryptdin-5. cryp4 and cryp5
oligonucleotides do not anneal to known defensin sequences, but cryp1
is an exact complement to the sequence for cryptdin-6, -9 to -11, -15, and -17 cDNAs; therefore, these isoforms would be detected under the
assay conditions described (4). PCR were performed using a
program of 15-23 cycles at 94°C for 40 s, 60°C for
40 s, and 72°C for 40 s. Murine -actin cDNA was also
amplified as an internal standard using the primer sets
(5'-CAACTGGGACGACATGGAGAAGA-3', corresponding to nucleotides
311-323; 5'-CAATAGTGATGACTTGGCCGTC-3', the reverse complement of
nucleotides 810-831) (29). To assess the relative intestinal levels of cryptdin mRNA expression, RT-PCR reactions were
sampled every two to three cycles. PCR amplification products were
subjected to 2% agarose gel electrophoresis, stained with ethidium
bromide, and viewed under ultraviolet light, and photographs were taken.
In situ hybridization. Ileal tissues were obtained from GF, SPF, and 3-day bacteria-reconstituted mice, fixed in 4% paraformaldehyde solution containing 0.5% glutaraldehyde, and embedded in paraffin wax. Cryptdin-5 cDNA was obtained by RT-PCR as described in Detection of murine cryptdin isoform and subcloned into pGEM-T Easy Vector (Promega). Digoxigenin-labeled antisense and sense cRNAs were synthesized from the subcloned plasmid after linearization using a DIG RNA labeling kit (Boehringer Mannheim, Indianapolis, IN) according to the manufacturer's instructions. Because cryptdin-5 cDNA is highly (>80%) homologous to other cryptdin cDNAs, it is possible for the antisense cRNA probe to hybridize to other cryptdin mRNAs (23). Two-micrometer-thick sections were covered with hybridization buffer containing antisense or sense probe (0.5 µg/ml). Hybridization was allowed to proceed at 45°C for 16 h, and then sections were washed twice for 60 min in 50% formamide containing 2× saline sodium citrate (SSC) at 45°C and once for 60 min in 50% formamide containing 1× SSC at 45°C. The hybridized digoxigenin-labeled probes were visualized using antidigoxigenin Fab fragment (Boehringer Mannheim) and BCIP-NBT substrate. The sections were developed in substrate solution for 3 h, washed in PBS buffer, and then counterstained with methyl green.
Immunohistochemistry. Three-micrometer-thick sections were mounted on silane-coated glass slides and incubated with a monoclonal antibody against murine serum amyloid A (SAA) at a dilution of 1:800 overnight at 4°C. This antibody is a rat-mouse chimeric antibody (kindly provided by Dr. T. Yamada, Juntendo Medical School, Tokyo, Japan) obtained by immunizing BALB/c mice with recombinant mouse SAA1 followed by fusion to mouse myeloma cell SP2. The reacting epitope of the antibody was suspected to be somewhere between residue 76 and the carboxyl terminus of SAA (36). Biotin-labeled anti-rat IgG (Dako Japan, Tokyo, Japan) was used at a dilution of 1:400 as a second antibody. Peroxidase-labeled avidin was obtained from Nichirei (Tokyo, Japan), and the antigen-antibody complex was visualized with 3,3'-diaminobenzidine (DAB) solution and counterstained with hematoxylin. The specificity of the immunohistochemical staining was confirmed by replacing the primary antibody with normal mouse IgG or PBS.
![]() |
RESULTS |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Serological and general assessment.
Bacteria-reconstituted mice gained weight similarly to their control
SPF counterparts. No mice died. There was no significant abnormality in
serum total proteins, albumin, GOT, GPT, or LDH except for CRP among
GF, conventionalized, and SPF mice (Table 1). Stool was softer in mice 3 days after
bacterial reconstitution compared with mice in other groups. Bloody
stool was not noticed in any mouse.
|
Histological assessment.
The villi in the small intestine of GF mice were short and slender, and
the lamina propria was underdeveloped compared with that of SPF mice
(Fig. 1, A and D).
After bacterial reconstitution, histological findings of small
intestinal mucosa were comparable to those of SPF mice (Fig. 1,
B and C). Paneth cells were recognized as
granulated epithelial cells in the small intestine and were found at
0-5 cells per crypt throughout the series of experiments. Colonic
mucosa of GF mice was characterized by atrophy of the epithelium and
minimal infiltration of cells into the lamina propria (Fig.
2A). In contrast to the case
of minor morphological alteration in the small intestine, an acute
inflammatory change developed in the colonic mucosa 3 days after
bacterial reconstitution, characterized by hyperplastic changes of
epithelial cells, infiltration of mono- and polymorphonuclear cells,
and edema in both the mucosa and submucosa (Fig. 2B).
Mucosal inflammation was remarkable at 7 days and reduced at 14 days,
exhibiting histological findings similar to those obtained in SPF (Fig.
2C). No ulceration was observed in any lesion of any group.
|
|
Summary of differential display.
A total of 60 displays with different primer sets were carried out. The
reproducibility of the displayed pattern was confirmed by three
individual amplifications using the same RNA source (data not shown).
Most of the bands appeared in both small intestine and colon regardless
of bacterial reconstitution. Forty-three bands were selected
based on the criteria described and categorized in Table
2.
|
Detection of small intestine-specific gene induction in
differential display.
In the display with the combination of the primer GT15C and GTTGCGATCC,
small intestine-specific bands, with enhancement at 3 days, were
detected (Fig. 3A). The cDNA
was 180 bp in length and was 99% homologous to nucleotides
259-438 of cryptdin-related sequence 4C (CRS4C). Northern blotting
confirmed the preferential expression of CRS4C in the small intestine
and induction at 3 days (Fig. 3B). In another display, with
the combination of the primer GT15C and TGCTCTGCCC, a pattern similar
to CRS4C was noticed. The cloned cDNA had a 99% homologous sequence to
nucleotides 20-265 of cryptdin-17 (data not shown).
|
Cryptdin isoform mRNAs in murine intestinal epithelial cells.
The amplified product for the cryptdin-4 isoform was of the expected
size and was most strongly detected at 3 days, as demonstrated by
semiquantitative RT-PCR (Fig. 4). Similar
results were obtained from the gene amplification for cryptdin-1 and -5 isoforms (data not shown).
|
In situ study for cryptdin mRNA expression in ileum.
When the ileum from GF mice was examined, a few cells, which were
present at the bottom of the crypt, exhibited weak but positive staining for cryptdin-5 (Fig.
5A). In contrast, the number
of positive cells increased and was approximately four or five per crypt, when we examined the ileum from 3-day bacteria-reconstituted mice (Fig. 5C). Similar results were obtained using the
ileum from SPF mice. Positive cells were found only at the bottom of the crypt in the series of experiments. When the sense probe was used,
positive staining was not detected in any of the samples (Fig. 5,
B, D, and F).
|
Detection of colon-specific gene induction by differential display.
In the display with the combination of primer GT15C and TCTGTGCTGG,
colon-specific bands, with enhancement at 3, 7, and 14 days, were
detected (Fig. 6A). Cloning
and sequencing revealed that the cDNA was 160 bp in length and was 99%
homologous to nucleotides 371-530 of murine serum amyloid A 1 (SAA1). Northern blotting using this clone as a probe confirmed
preferential and induced expression of SAA1 gene in the colon (Fig.
6B).
|
Immunoreactivity for SAA in colon.
To investigate the expression of SAA protein, an immunohistochemical
technique was used. Only weak staining was observed in epithelial cells
at the luminal surface of the rectum from GF and SPF mice (Fig.
7, A and C). In
contrast, when GF mice were given luminal bacteria and reared under SPF
conditions for 7 days, strong immunoreactivity was observed in the
cytoplasm of the entire epithelium, from the top to around the bottom
of the crypts (Fig. 7B). Similar results were obtained when
the cecum and the transverse colon were examined (data not shown).
|
![]() |
DISCUSSION |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
Bacterial reconstitution resulted in an acute, severe, but self-limited mucosal inflammation in mice. The trigger of mucosal inflammation was the first exposure of conventional bacteria in GF mice. This suggests that constitutively expressed molecules in GF mice failed to prevent inflammatory responses and maintain homeostasis. Although the ratio of each bacterial species in colonic microflora is different between mice after bacterial colonization and in SPF counterparts, the bacterial composition immediately becomes comparable within a few weeks (8, 19). Our preliminary data exploring four representative bacterial species have also shown transient increase of Enterobacteriaceae and Streptococcus in feces 3 and 7 days after bacterial reconstitution. In addition, Lactobacillus and Bacteroides were increased in number in the process (8). Conventionalized mice no longer exhibited mucosal inflammation at 2 wk regardless of the presence of conventional bacteria. This is critical because conventionalized mice with a genetically normal background could develop and maintain a protective system against luminal bacteria, although we did not identify the bacterial species responsible for triggering the mucosal inflammation and recovery.
This functional alteration possibly requires induction or suppression of genes to synthesize functional proteins, which are essential for the establishment of mucosal defense. To analyze this process, we applied differential display by using separately isolated small intestinal and colonic epithelial cells as RNA sources.
Differential display is a recently described tool that detects altered gene expression (18). This approach has been quite successful in identifying a number of new genes (1, 5, 17, 27, 33, 37). Advantages of this method are the requirement of a small amount of RNA and the ease of visual comparison among many samples. This enables us to analyze at one time the RNA expression in small intestinal and colonic epithelial cells harvested at different time intervals. Although another novel approach, which uses cDNA array, is applicable for gene screening, this method does not easily compare gene composition in many samples (15).
In our 60 differential displays, most of the bands appeared in both small intestine and colon regardless of bacterial reconstitution. A total of 43 bands were selected as candidates for differentially expressed genes, and differential expression of CRS4C and SAA1 genes was confirmed in this study.
CRS4C was detected as a small intestine-specific gene. This molecule is one of the cryptdin-related sequences of unknown function (6, 12, 23). Cryptdins are constitutive members of the defensin family, and their selective expression in Paneth cells has been identified (24). To our knowledge, antimicrobial activities of cryptdins have been shown only in vitro (6, 23), and critical factors that induce cryptdin genes are still unknown. In particular, microbial antigens have not been considered as activators (4) because cryptdin mRNA in the small intestine of GF mice was as abundant as in conventional mice (22). In contrast, CRS4C and cryptdin-4 mRNAs were clearly induced at 3 days after bacterial reconstruction in small intestinal epithelial cells. The enhanced expression of cryptdin mRNA was caused by the increased number of expressing cells, demonstrated in part by in situ hybridization. Because the number of cryptdin mRNA-expressing cells was comparable between SPF and bacteria-reconstituted mice, differential mRNA expression detected by Northern blot and RT-PCR suggests that the degree of cryptdin mRNA expression in each cell may be different between the two groups. Although induction mechanisms of cryptdins remain unknown, antibacterial peptide genes were actually inducible in association with luminal bacteria in vivo. This observation also suggests that our approach of displaying time-dependent gene expression provides more information than a random comparison of gene expression in GF and SPF conditions.
SAA1 was detected as a colon-specific gene with enhancement of its mRNA
level at 3 and 7 days after bacterial reconstitution. Induction of SAA
was also observed at the protein level, as demonstrated by
immunohistochemistry. SAA1 is an acute-phase protein mainly produced in
the liver (32). Because IL-1, IL-6, and
lipopolysaccharide (LPS) induced SAA gene in hepatic and other cells
(16, 31), SAA1 might be induced locally by
direct exposure to these cytokines and/or LPS. Therefore, this
observation indicates that epithelial gene expression modulated by
soluble factors is also detectable by the approach described here.
Additionally, if SAA induction was mediated by soluble factors such as
IL-1
, IL-6, and LPS, enhancement of epithelial SAA mRNA level might
be relevant to other experimental colitis and human IBD because
enhanced gene and protein expression of proinflammatory cytokines have
been well established in mucosal inflammation (7). The
functional properties of this molecule still remain unclear; however,
the chemoattractive functions of T cells, monocytes, or neutrophils have been reported (2, 34). Furthermore, the
binding capacity of SAA to LPS may suggest a possible defensive role of
the mucosa to a bacterial insult. In fact, the recombinant SAA1
inhibits cytokine secretion in LPS-stimulated mononuclear cells
(unpublished data).
Our approach highlights several significant future directions. Although how the detected genes contribute to mucosal defense and the inflammatory process remains unknown, investigation of the expression of these genes in other experimental colitis models will likely be able to demonstrate the relevance and significance of the genes involved in mucosal inflammation. Gene expression of human homologues of selected genes may be easily investigated using epithelial RNAs from human IBD patients to show possible modulation of mucosal defense-associated genes (10, 20). We inoculated multiple bacterial species prepared from SPF mice into GF mice; however, it is also important to determine which bacterial species are essential for the induction or modulation of selected genes. Inoculation of a single species such as Bacteroides may contribute to an answer to this fundamental question. Moreover, this bacterial reconstitution model is also applicable to analysis of immune activation and the anergy of mucosal lymphocytes and macrophages against normal enteric bacteria.
In conclusion, CRS4C, cryptdin, and SAA1 have been identified as molecules associated with the mucosal defense system against luminal microorganisms and the mucosal inflammation triggered by them through the new approach described here. Further investigation should lead to a better understanding of the role of luminal bacteria in the generation and amplification of mucosal inflammation in experimental and human IBD.
![]() |
ACKNOWLEDGEMENTS |
---|
This work is supported by a grant from the Ministry of Education, Science and Culture of Japan (10470252), the Yakult Bioscience Foundation, and the Crohn and Colitis Foundation of America.
![]() |
FOOTNOTES |
---|
Address for reprint requests and other correspondence: K. Fukushima, Dept. of Surgery, Tohoku Univ. School of Medicine, 1-1 Seiryou-machi, Sendai, Japan 980-8574 (E-mail: kouhei{at}gonryo.med.tohoku.ac.jp).
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Received 8 October 1999; accepted in final form 8 March 2000.
![]() |
REFERENCES |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
1.
Aiello, LP,
Robinson GS,
Lin YW,
Nishio Y,
and
King GL.
Identification of multiple genes in bovine retinal pericytes altered by exposure to elevated levels of glucose by using mRNA.
Proc Natl Acad Sci USA
91:
6231-6235,
1994[Abstract].
2.
Badolato, R,
Wang JM,
Murphy WJ,
Lloyd AR,
Michiel DF,
Bausserman LL,
Kelvin DJ,
and
Oppenheim JJ.
Serum amyloid A is a chemoattractant: induction of migration, adhesion, and tissue infiltration of monocytes and polymorphonuclear leukocytes.
J Exp Med
180:
203-209,
1994[Abstract].
3.
Chadwick, VS,
and
Anderson RP.
Microorganisms and their products in inflammatory bowel disease.
In: Inflammatory Bowel Disease. New York: Elsevier Science, 1992, p. 241-258.
4.
Darmoul, D,
Brown D,
Selsted ME,
and
Ouellette AJ.
Cryptdin gene expression in developing mouse small intestine.
Am J Physiol Gastrointest Liver Physiol
272:
G197-G206,
1997
5.
Donohue, PJ,
Alberts GF,
Hampton BS,
and
Winkles JA.
A delayed-early gene activated by fibroblast growth factor-1 encodes a protein related to aldose reductase.
J Biol Chem
269:
8604-8609,
1994
6.
Eisenhauer, PB,
Harwig SS,
and
Lehrer RI.
Cryptdins: antimicrobial defensins of the murine small intestine.
Infect Immun
60:
3556-3565,
1992[Abstract].
7.
Fiocchi, C.
Inflammatory bowel disease: etiology and pathogenesis.
Gastroenterology
115:
182-205,
1998[ISI][Medline].
8.
Fukushima, K,
Sasaki I,
Ogawa H,
Naito H,
Funayama Y,
and
Matsuno S.
Colonization of microflora in mice; mucosal defense against luminal bacteria.
J Gastroenterol
34:
54-60,
1999[ISI][Medline].
9.
Fukushima, K,
Sasaki I,
Hasegawa H,
Takahashi K,
Naito H,
Funayama Y,
and
Matsuno S.
Sodium butyrate-induced liver-type alkaline phosphatase activity in a small intestinal epithelial cell line, IEC6.
Dig Dis Sci
43:
1116-1123,
1998[ISI][Medline].
10.
Fukushima, K,
Sasaki I,
Ogawa H,
Takahashi K,
Yamada T,
Naito H,
and
Matsuno S.
Enhanced epithelial expression of serum amyloid A in inflammatory bowel disease (IBD) (Abstract).
Gastroenterology
116:
A720,
1999.
11.
Hammer, RE,
Maika SD,
Richardson JA,
Tang JP,
and
Taurog JD.
Spontaneous inflammatory disease in transgenic rats expressing HLA-B27 and human 2-microglobulin: an animal model of HLA-B27-associated human disorders.
Cell
63:
1099-1112,
1990[ISI][Medline].
12.
Huttner, KM,
Selsted ME,
and
Ouellette AJ.
Structure and diversity of the murine cryptdin gene family.
Genomics
19:
448-453,
1994[ISI][Medline].
13.
Ishii, S,
Steele G, Jr,
Ford R,
Paliotti G,
Thomas P,
Andrews C,
Hansen HJ,
Goldenberg DM,
and
Jessup JM.
Normal colonic epithelium adheres to carcinoembryonic antigen and type 4 collagen.
Gastroenterology
106:
1242-1250,
1994[ISI][Medline].
14.
Ito, T,
Kito K,
Adati N,
Mitsui Y,
Hagiwara H,
and
Sakaki Y.
Fluorescent differential display.
FEBS Lett
351:
231-236,
1994[ISI][Medline].
15.
Johnston, M.
Gene chips: array of hope for understanding gene regulation.
Curr Biol
8:
R171-R174,
1998[ISI][Medline].
16.
Kumon, Y,
Sipe JD,
Brinckerhoff CE,
and
Schreiber BM.
Regulation of extrahepatic apolipoprotein serum amyloid A (ApoSAA) gene expression by interleukin-1 alone: synthesis and secretion of ApoSAA by cultured aortic smooth muscle cells.
Scand J Immunol
46:
284-291,
1997[ISI][Medline].
17.
Liang, P,
Averboukh L,
Keyomarsi K,
Sager R,
and
Pardee AB.
Differential display and cloning of messenger RNAs from human breast cancer versus mammary epithelial cells.
Cancer Res
52:
6966-6968,
1992[Abstract].
18.
Liang, P,
and
Pardee AB.
Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.
Science
257:
967-971,
1992[ISI][Medline].
19.
Maejima, K,
Sasaki J,
Shimoda K,
and
Kurosawa T.
Bacterial flora of ex-germfree mice after oral inoculation of feces from various species of conventional animals.
Exp Anim
30:
157-160,
1981.
20.
Ogawa, H,
Fukushima K,
Sasaki I,
Takahashi K,
Naito H,
Funayama Y,
and
Matsuno S.
Preferential expression of HIP/PAP gene in colonic epithelial cells (EC) of inflammatory bowel disease (IBD) (Abstract).
Gastroenterology
116:
A786,
1999[ISI].
21.
Onderdonk, AB,
Cisneros RL,
and
Bronson RT.
Enhancement of experimental ulcerative colitis by immunization with Bacteroides vulgatus.
Infect Immun
42:
783-788,
1983[ISI][Medline].
22.
Ouellette, AJ,
Greco RM,
James M,
Frederick D,
Naftilan J,
and
Fallon JT.
Developmental regulation of cryptdin, a corticostatin/defensin precursor mRNA in mouse small intestinal crypt epithelium.
J Cell Biol
108:
1687-1695,
1989[Abstract].
23.
Ouellette, AJ,
Hsieh MM,
Nosek MT,
Cano-Gauci DF,
Huttner KM,
Buick RN,
and
Selsted ME.
Mouse Paneth cell defensins: primary structures and antibacterial activities of numerous cryptdin isoforms.
Infect Immun
62:
5040-5047,
1994[Abstract].
24.
Ouellette, AJ,
and
Selsted ME.
Paneth cell defensins: endogenous peptide components of intestinal host defense.
FASEB J
10:
1280-1289,
1996
25.
Robert, A,
and
Asano T.
Resistance of germfree rats to indomethacin-induced intestinal lesions.
Prostaglandins
14:
333-341,
1977[Medline].
26.
Sadlack, B,
Mertz H,
Schorle H,
Schimpl A,
Feller AC,
and
Horak I.
Ulcerative colitis-like disease in mice with a disrupted interleukin-2 gene.
Cell
75:
253-261,
1993[ISI][Medline].
27.
Sager, R,
Anisowicz A,
Neveu M,
Liang P,
and
Sotiropoulou G.
Identification by differential display of alpha 6 integrin as a candidate tumor suppressor gene.
FASEB J
7:
964-970,
1993
28.
Sambrook, J,
Fritsch EF,
and
Maniatis T.
Molecular Cloning. A Laboratory Manual (2nd ed.). Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1989, p. 7.19-7.22.
29.
Screaton, GR,
Bell MV,
Bell JI,
and
Jackson DG.
The identification of a new alternative exon with highly restricted tissue expression in transcripts encoding the mouse Pgp-1 (CD44) homing receptor.
J Biol Chem
268:
12235-12238,
1993
30.
Sellon, RK,
Tonkonogy S,
Schultz M,
Dieleman LA,
Grenther W,
Balish E,
Rennick DM,
and
Sartor RB.
Resident enteric bacteria are necessary for development of spontaneous colitis and immune system activation in interleukin-10-deficient mice.
Infect Immun
66:
5225-5231,
1998.
31.
Steel, DM,
Donoghue FC,
O'Neill RM,
Uhlar CM,
and
Whitehead AS.
Expression and regulation of constitutive and acute phase serum amyloid A mRNA in hepatic and non-hepatic cell lines.
Scand J Immunol
46:
284-291,
1996[ISI].
32.
Steel, D,
and
Whitehead AS.
The major acute phase reactants: C-reactive protein, serum amyloid P component and serum amyloid A protein.
Immunol Today
15:
81-88,
1994[ISI][Medline].
33.
Utans, U,
Liang P,
Wyner LR,
Karnovsky MJ,
and
Russell ME.
Chronic cardiac rejection: identification of five up-regulated genes in transplanted hearts by differential mRNA display.
Proc Natl Acad Sci USA
91:
6463-6467,
1994[Abstract].
34.
Xu, L,
Badolato R,
Murphy WJ,
Oppenheim JJ,
and
Wang JM.
A novel biologic function of serum amyloid A.
J Immunol
155:
1184-1190,
1995[Abstract].
35.
Yamada, M,
Ohkusa T,
and
Okayasu I.
Occurrence of dysplasia and adenocarcinoma after experimental chronic ulcerative colitis in hamsters induced by dextran sulfate sodium.
Gut
33:
1521-1527,
1992[Abstract].
36.
Yamada, T,
Fukuda T,
Wada A,
and
Itoh Y.
Monoclonal antibody-based sensitive enzyme linked immunosorbent assay for murine serum amyloid A.
J Immunoassay
20:
223-235,
1999[ISI][Medline].
37.
Zimmermann, JW,
and
Schultz RM.
Analysis of gene expression in preimplantation mouse embryo: use of mRNA differential display.
Proc Natl Acad Sci USA
91:
5456-5460,
1994[Abstract].